LOCUS       DASTJP010000001         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_528092, whole genome shotgun sequence.
ACCESSION   DASTJP010000001 DASTJP010000000
VERSION     DASTJP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528092"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VFQ23_00005"
     CDS             complement(<1..1302)
                     /locus_tag="VFQ23_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9904978.1"
                     /translation="MILLLYFLYGLAFEGLGLAAYLQLRREGDFPLKKELPWLAAFGF
                     VGGAAGWVDMFLNSGSLNEIAGVLSILRVIAHLLTGLLLLRFGWGMLNNLDPLPAWTI
                     FIPGILIVPIAFIITYAATTFITPSPIEIPIEIWTRYLLYLPGSLLAGVGFLRQWHAQ
                     RKLALYDVSNLMLGAGLAFLFEAVMVGLIVPAAPYGPASYYNYDRVIHNAFIGENLEV
                     THPYGLIPWLDYRSVLNTTGLPIEIWRLLSAVIVTFFMVKALDVFEAIRKRQILALQQ
                     ERDRAQRGAIEAQIIARQTAENWTNALVNISRRIAELEHVDDILAYIVDHIHQLLKTN
                     FVGVAIFNPENASLELKCFSNDGRAQLVSTPSVKVTNPLLWNVVQKSASYRAQGEEQP
                     EIFEGVCFFMEETAKTVVTVPLKLENTTIGVLWIARVDENHF"
     gene            complement(1718..>2209)
                     /locus_tag="VFQ23_00010"
     CDS             complement(1718..>2209)
                     /locus_tag="VFQ23_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9904979.1"
                     /translation="CGFYGDSQKPCTCAAPVVTKYQKRISGPLLDRIDIHIEVPRVDY
                     EKLSGDRVGETSGSIRARVQAARDIQTKRYSSLRNSNTDLSAIVCNADMRVGEIRQFC
                     KLPEDGQSLMRAAMGQLNLSARAYHRILKLARTIADLAGSGEIQSVYLAEALQYRPKL
                     LLS"
BASE COUNT          571 a          588 c          524 g          526 t
ORIGIN      
        1 aaagtgattc tcatcgacac gcgcaatcca caggacaccg atggttgtat tctccagctt
       61 gagtggcaca gtgacaactg ttttagcagt ctcctccatg aagaaacaaa ctccttcgaa
      121 gatctccggc tgctcttcac cctgggcacg atatgaagca gatttctgca ccacgttcca
      181 gaggagcggg ttcgtcactt tgacggatgg ggttgacacc agctgtgctc ttccatcatt
      241 tgaaaagcac ttcaattcaa gtgaagcgtt ctcagggtta aagatcgcca cgccaacgaa
      301 attggtcttc agaagctgat ggatgtgatc cacgatgtag gccagaatgt cgtccacgtg
      361 ttccagctcg gcgatgcggc ggctgatatt gaccagcgcg tttgtccaat tttccgcggt
      421 ctgccgcgcg atgatctggg cttcaattgc tcccctttgt gcgcggtccc gttcctgttg
      481 aagggccagt atctgccgct tgcggatggc ttcaaagaca tccaacgctt tcaccataaa
      541 gaaggtcaca atcaccgctg ataacaaccg ccagatctca atcggcaggc cggtggtatt
      601 caacacgctt cgataatcaa gccagggaat taatccatag ggatgagtca cttcgagatt
      661 ctccccgata aatgcattgt ggatgacgcg gtcatagttg tagtatgagg caggtccata
      721 cggcgctgct gggacaatta gcccaaccat gacagcttca aacaggaaag ccagccctgc
      781 accgagcatc aaattcgata catcgtatag cgccagcttt cgttgggcat gccactgccg
      841 caaaaatcct actccagcca gtaaactgcc ggggagatat aacagatagc gtgtccaaat
      901 ttcgatgggg atctctatcg gggaaggcgt aataaatgtg gttgccgcgt aagtgatgat
      961 gaaggcgatc ggaacgatga ggatccccgg gataaaaatg gtccaggccg gaagggggtc
     1021 caggttgttc agcattcccc atccaaagcg caggagcagc aatccggtca ggagatgagc
     1081 gatgacacgt aggatgctta aaacaccggc aatctcattg agagatccac tattgaggaa
     1141 catatccacc cagcctgctg cgccacccac aaaaccaaag gctgccagcc acggaagttc
     1201 ctttttcagc ggaaaatcac cttctcgtct tagttgcaga tacgcggcta atcctaaccc
     1261 ctcaaaagcc agaccataga ggaaatatag gagcagtatc acaaggcttt ccgatcaagc
     1321 aaattttgtc ttttttgtga aatttttctc gatcatttta gcccccttcg tgctgaatag
     1381 caattgacta aaatcatgat ttgtttgtgt ctatcaatat tgaattcatg ctactcattc
     1441 gtcactaaaa ggctttattc cccaccatca acttggatct gtactgtaat gcaactgcca
     1501 cctctgcaaa cgtacgcgtt aagttcactc ctaccgcgtg atgataggcc cacgtcgaca
     1561 gatcagggtg acttaggcac atcagttgct ggttcccaga gattagttac cagaatgagt
     1621 agatacatct tgcagatggc agatcacaaa caaaatccgg attcgagcta tggcgcggat
     1681 atactcaaga tatgagcaga agcatgatgg gtcaaaacta actcagcaac aacttcggcc
     1741 gatattgcaa cgcctctgcc agatacacag actggatctc cccactcccc gccaggtccg
     1801 caatcgtgcg cgccagcttg aggatgcgat gataagctcg cgcagataaa ttgagctggc
     1861 ccatcgccgc tcgcatcagg ctttgaccgt cttccggtaa cttgcagaac tgccggatct
     1921 cccccacgcg catgtctgcg ttacaaacga tagccgataa gtcggtattc gaatttcgaa
     1981 gactcgagta tctttttgtt tgtatatccc gcgcagcctg gactcgcgct cgaatcgatc
     2041 cgctcgtctc acccactcga tccccactca gtttctcata atccacgcgc ggcacttcga
     2101 tgtgaatatc gatgcggtcg agcaatggac cggagatacg tttctgatat ttggtcacga
     2161 caggagccgc acaggtacaa ggcttctggc tgtccccata aaatccgca
//
LOCUS       DASTJP010000002         3810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_738085, whole genome shotgun sequence.
ACCESSION   DASTJP010000002 DASTJP010000000
VERSION     DASTJP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738085"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFQ23_00015"
     CDS             complement(<1..356)
                     /locus_tag="VFQ23_00015"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9904980.1"
                     /translation="MNGLQLYFRLIQISLLSRIQYRANFIAGIFGLVFWNIINLGLIG
                     VLVSRFTNLNNWTVWELVFLYCLWILGHSLYGLFFSHTAQMEDYLIEGTFDQFLLRPA
                     SPLLQFLGRELQYIEIA"
     gene            complement(372..1163)
                     /locus_tag="VFQ23_00020"
     CDS             complement(372..1163)
                     /locus_tag="VFQ23_00020"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9904981.1"
                     /translation="MKILWAFARQNFQVYSIYRLDFLLRSSSLLILMFGIRWVWITLY
                     TQRPEAFGVTLQQMVTYGVLSIAIQNLFFTGPQYYIARQVRSGAIDSDLLKPLDFHFH
                     MLARSAGEMLLRIVVIAIPGVLVGYFLFDLQFPTNSKAGFMFVVALLLGFLVNFHLDF
                     LLGAISVVTMEINSIDWAFHATSRFFSGQFLPLWLFPGVLRTLAEILPFRSVFFIPLS
                     IYNGTLSGDALREAIKFQLLWLIILLMISRWVWERVQGRLVSQGG"
     gene            complement(1197..1997)
                     /locus_tag="VFQ23_00025"
     CDS             complement(1197..1997)
                     /locus_tag="VFQ23_00025"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9904982.1"
                     /translation="MNALTLYFRLIRVAWQARLQYRADFFTGVIGVIVLNLVNVSLIG
                     ILVSRFQHLNGWSLWEMVFLYCLWLLGHSFYSFFFWHIRALEDYLVEGTFDQFLMRPA
                     SPFIMFLGREVQYLGIADLIFSVLGLSLAYQNLHLEWTPLQWLFFGAAVVSGALIETT
                     LVLAIACISFWTGRSRRAHQLLTRLNMMVQYYPVDIFGYVFRVIVTGLLPVAFMNYYP
                     SLMLLGKLDIQSPWGWLGYMSPVVALMLVLITSSIWNLALRRYSSSGG"
     gene            complement(1998..2789)
                     /locus_tag="VFQ23_00030"
     CDS             complement(1998..2789)
                     /locus_tag="VFQ23_00030"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9904983.1"
                     /translation="MKILWAFARQVFHGNAIYRFEFWMRLFWVYMLMYSIYWVWKTLY
                     TQTPGAFGVSLQQMVTYGILGMALDIFLDVGPEWYMATQVRTGAIDTDLMKPLDFHLH
                     MLARSAGEMMFSLVILALPAFIIAYFLFDLRLPGDGLTTLLFGISLVLSFFVFFHISF
                     LLGTLTVVTLDIRSISWAYYSVVSFFSGQIVPLWLFPDLLRKISELLPFQAVYYIPMS
                     IYIKTLSGKDAIQALGVQVFWTVVLVLISRWAWVKVHTRLTVQGG"
     gene            complement(2786..3784)
                     /locus_tag="VFQ23_00035"
     CDS             complement(2786..3784)
                     /locus_tag="VFQ23_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9904984.1"
                     /translation="MIKVKDLTKDYRRVKRREGFLGSVRTLFSAEYETTRAVDNISFE
                     IQEGELVGYIGPNGAGKSTSIKMLCGILVPSNGEITVNGLVPHKSRMENTRQIGAVFG
                     QKTQLWWDVPVIESLKLFRDIYKVPEAQYKRNLTLFNDLLDLNEFQNTPVRQLSLGQR
                     LRADMAAALLHNPRLLFLDEPMIGVDVVAKEKLREFIKEINRDQKVTILLTTHDMTDI
                     EKVCTRIMIIDHGRIVYDGSLEQIRAEYGKQRKLVVEFEEEVPDFSAPYATLSKSEGK
                     KKWFAFNRFETSPSTLIADISSRFPIIDLAVEEPEIEEIVRTIYRGKSETPDLPAK"
BASE COUNT         1149 a          950 c          895 g          816 t
ORIGIN      
        1 gcaatttcaa tatattgcaa ttcacggccc aggaattgga gcaatgggct ggccgggcga
       61 agaaggaatt ggtcaaacgt cccctcgatc agataatctt ccatctgagc ggtatgtgag
      121 aagaacagac catacagact atgcccaaga atccacaagc aatatagaaa tacgagctcc
      181 cagacggtcc aattgttcag attcgtaaag cggctgacaa gcacaccgat caaccccagg
      241 ttgataatgt tccagaacac caatccaaaa atgccagcaa taaaatttgc tcgatactgt
      301 atgcgcgata gcagcgagat ttgaatcaac cgaaaataca attgaagtcc gttcatcttt
      361 gcgtttgcgc ctcaacctcc ctgcgatacc aaccgcccct ggactcgttc ccagacccag
      421 cggcttatca tcagcagaat gatcagccac aacagttgaa actttatcgc ctctctcagc
      481 gcatccccgc tcaatgtacc gttatagatt gagagtggga tgaagaacac cgaacgaaat
      541 ggaagaattt cagccagcgt tctcaaaact cccggaaata accaaagcgg caagaactgt
      601 ccagagaaaa agcgcgaggt tgcatgaaat gcccaatcaa tactgttgat ttccatggtg
      661 acgactgaaa tagcacccag caaaaaatcg agatggaaat tgaccagaaa cccgagaagg
      721 agggcaacca caaacatgaa tccagctttg gagtttgtag ggaattggag atcgaaaagg
      781 aaatatccca ccagtacgcc aggaatggct atgaccacaa tgcgcaataa catctctccg
      841 gcgctgcgcg ctaacatgtg aaaatgaaaa tccagaggtt tcaacagatc agaatcaatt
      901 gcaccactgc gcacctggcg tgcgatgtaa tattgcggac ccgtaaagaa aaggttttgg
      961 attgcgatac tcaacactcc gtacgttacc atctgttgca acgtgacgcc gaaagcctca
     1021 gggcgctgtg tgtaaagcgt gatccaaacc caccgaatgc caaacatcag aatcaagaga
     1081 gaggaggacc gcaataaaaa atcaaggcgg taaatggaat aaacctgaaa attctgccgg
     1141 gcaaatgccc aaaggatttt cacgtacgca gctccgagtt tcgtctcaaa cgatctttac
     1201 ccaccagaac tggaatagcg acggagcgcc agattccaaa tactggaagt aatcaagacc
     1261 agcatcaagg caaccacggg cgacatataa cccagccaac cccacggact ttgaatgtcg
     1321 agtttgccaa ggagcatcaa tgaaggatag taattcatga aggcgaccgg aagcaaaccg
     1381 gtaacaatga cccgaaacac atacccgaaa atatcgaccg ggtagtattg gaccatcata
     1441 ttaagtcgag tcagaagttg atgcgctcgg cgagagcgac ctgtccaaaa cgaaatacaa
     1501 gcgattgcca ataccagcgt ggtttcgatg agtgctccgg agacaaccgc tgccccaaag
     1561 aaaagccact gtaacggggt ccattccagg tgcagatttt ggtaggccag gctcaacccc
     1621 agaacggaaa agatcaggtc tgcaataccg agatattgaa cctcgcgccc gaggaacata
     1681 ataaagggac ttgccgggcg catgaggaac tgatcaaacg ttccctccac gagatagtct
     1741 tccagagcac gaatgtgcca gaagaaaaaa ctataaaagc tatgtcctaa aagccataga
     1801 caataaagaa acaccatctc ccacagggac cagccgttca ggtgttggaa gcggctgaca
     1861 agaatgccga tcaggcttac attgacaagg ttaagaacga tgacgcctat cacacccgtg
     1921 aaaaagtccg cgcggtactg taagcgcgcc tgccaggcga cacggatcaa tctgaagtaa
     1981 agagtgagcg cgttcaatca gccgccttga acggttagac gcgtatgaac cttcacccag
     2041 gcccagcgtg aaataagaac gagcaccact gtccaaaata cctgtacccc taatgcctgg
     2101 atagcgtcct tgccactcaa tgtcttgata tagatggaca tgggaatgta ataaacagcc
     2161 tgaaaaggca gaagttcgga aatcttgcgg agaagatcgg ggaagagcca caaaggaacg
     2221 atttgcccgg agaaaaacga gaccaccgaa taataagccc agctgatgct gcgaatgtca
     2281 agcgtgacga ccgtcaatgt gccgagcaga aagctaatgt gaaagaagac aaaaaagcta
     2341 aggaccaggc ttatcccaaa aagtaatgtc gtgagtccat cgcccggcaa ccgcaagtcg
     2401 aaaagaaaat aggcgatgat aaaggccggc aatgccagga tgacaagact aaacatcatc
     2461 tcaccggcgc tacgcgccaa catgtgcaag tgaaaatcca acggcttcat caggtccgtg
     2521 tcaatcgcgc ccgtacgaac ctgtgttgcc atataccatt ctgggcccac atccaggaaa
     2581 atatcaagcg ccatgcccaa aataccgtat gtcaccattt gctggaggct gacgccgaaa
     2641 gcgcccgggg tttgcgtgta gagagttttc catacccaat atatgctata catgagcata
     2701 tacacccaga agagccgcat ccaaaattca aagcggtaga tggcatttcc gtgaaatacc
     2761 tgtctcgcaa atgcccacag tattttcatt tggctgggag gtcaggggtt tccgatttgc
     2821 ctcgataaat ggttcgcaca atctcctcga tctccggttc ttccaccgcc agatcaatga
     2881 tcgggaagcg ggaactaata tcggcgatta gtgtggaggg cgaagtttca aagcgattga
     2941 aggcaaacca cttcttcttt ccctcgcttt ttgaaagcgt cgcataaggc gcggagaaat
     3001 cgggcacttc ttcttcgaac tcaacgacga gcttccgttg tttgccatat tcggcgcgga
     3061 tctgctcgag actgccatca tagacaatgc gaccatggtc gatgatcatg atgcgagtgc
     3121 aaaccttctc gatatccgtc atatcatggg tggtgagcag gatggtcact ttttggtcgc
     3181 ggttgatctc tttgatgaat tcacgcagtt tctctttcgc cacaacatca acaccaatca
     3241 tgggctcatc caaaaaaagc agacgcgggt tgtgaagcaa agcggcagcc atatcagcgc
     3301 gcagacgttg acctaaactt aactgccgaa ccggtgtatt ttggaactca tttagatcaa
     3361 gtaagtcatt gaagagagtt aaattgcgtt tatattgggc ttctggcact ttatagatat
     3421 cgcggaataa tttcaggctt tcaatgaccg gcacatccca ccagagctgg gtcttctgac
     3481 cgaacactgc tccgatctgg cgcgtgttct ccatccgact tttatgaggg acgagtccat
     3541 tcacggtgat ctcgccattg cttggaacca gaattccaca caacattttg atcgatgtgg
     3601 attttcctgc gccattcggc ccgatataac ctaccaactc gccttcttgt atttcaaagg
     3661 agatattatc gacggcgcgt gtggtctcat attcggcgct gaataaggtg cggacgctgc
     3721 caaggaaacc ttcccgacgt ttgacgcggc gatagtcttt tgttaagtct ttaactttga
     3781 tcatagtccc tcgaattatt tacattttag
//
LOCUS       DASTJP010000003         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1267158, whole genome shotgun sequence.
ACCESSION   DASTJP010000003 DASTJP010000000
VERSION     DASTJP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267158"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VFQ23_00040"
     CDS             <1..1545
                     /locus_tag="VFQ23_00040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9904985.1"
                     /translation="ERIIAVGDADDLSSQYPNAEKQDMQRRFILPGLTDAHLHLHHYA
                     LSLQKIDCETATKEECLRRIEERVKTAKPGEWILGHGWNQNVWAANPPLPVGEGLGVR
                     GWPHASEIDAIAPNNPVYLTAKSLHAAWANTSALKLANISTQTPDPQNGQIQRDANGN
                     ATGVLLETAMELVGKVIPAATIEEIAGAIEKAQSTLWKVGVTGVHDFDRRESFMALQQ
                     LHRHSKLKLRVLKNIPVELLDQVFELGLGAGFGDDWLRIGSVKVFMDGALGPHTAAMF
                     QPYIGEGENRGILNMDGEELFEHGRKAAQVGLGMTVHAIGDRANHEVLNAYEALRTYE
                     KENHLPALRHRIEHVQVIHPDDAPRLGRMNIIASMQPIHATSDMLMADQFWGERSRLA
                     YAMRTQLDFGARLALGSDAPVESPNPFWGLHAAVTRQRADGSPSPDGWYPEQKLTMAE
                     AVAGYTSGPAYTANMEDRLGKLAANYLADLIVLEKDIFTCNPNDLLTLESSATMIGGE
                     WVWEKP"
     gene            1649..2668
                     /locus_tag="VFQ23_00045"
     CDS             1649..2668
                     /locus_tag="VFQ23_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9904986.1"
                     /translation="MAEQPRLTPEMLVPRLGEYLIQKGLIDTASLQKALDYQQEEVSK
                     GNSVMLGQALIDQQLIDRAALDQAVTEQILQLRSALQSANRTLERRVEERTAELQKAL
                     ERVSELSQLKANFISNISHELRTPLTHVKGYVELLITGSMGPITDEQRHALQVSQQST
                     LRLEMLIEDLIMVSLASRGELSINQRDVDIRRLANLAAKSSSEKAQQRGVNLHAVIDG
                     NLPLVQADSQKIAWVLNQLIDNGIKFTPAGGSVVVSVQREGENLVLVSVTDTGIGIPI
                     NKMKDIFEPFHQLDGSSTRRYGGTGLGLSLVRDIIEAHGSMLDVQSTEGRGTTFKFPL
                     LITVN"
     gene            2678..>3211
                     /locus_tag="VFQ23_00050"
     CDS             2678..>3211
                     /locus_tag="VFQ23_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9904987.1"
                     /translation="MDYKTLEYLQELMRTTAFAENSRLVVENFLKTLREQFVFDNVAV
                     YVYDDSTQSLDVVYARAVGRAKTAEADAAWGETFAGQVLKSGKLLLQDPKADAPLDDR
                     LSRAYLLGLPLRSDIAIKGALIFVRFGGPVYESEHITLASFAADLISNLFERAAWKET
                     ISELSELKRQMQLQEDFV"
BASE COUNT          844 a          793 c          837 g          737 t
ORIGIN      
        1 gagcgaatca ttgcggtagg ggatgcagat gatttgtcgt cacaatatcc aaacgccgaa
       61 aaacaagata tgcagcggcg tttcatatta cctggactca cggatgcgca tttgcacttg
      121 catcattacg ctctcagttt acaaaagata gattgcgaaa cagcaacgaa ggaagaatgc
      181 ctgcgccgga tagaagagcg cgtaaagacg gcaaaacccg gtgaatggat tctgggtcac
      241 gggtggaacc aaaatgtatg ggcagcgaat ccccctctcc cggtgggaga ggggctaggg
      301 gtgaggggct ggccacacgc ttctgagata gatgcaatcg caccaaacaa ccctgtttac
      361 ctcacagcca aatccttgca tgcagcctgg gcaaatactt ccgcgctgaa gcttgctaac
      421 atttcaacac aaacacctga ccctcaaaac ggacagatcc aacgcgacgc gaacggcaat
      481 gcaactggag tcctgctcga aactgcgatg gaactggttg gtaaagttat tccagcagcg
      541 acgattgaag aaattgcagg tgccattgaa aaggcccagt ccaccttatg gaaggtaggc
      601 gtcacaggcg ttcacgattt cgacaggcgt gaatcgttca tggctttgca gcaacttcac
      661 cggcacagca aattaaaatt acgcgtgctt aagaacatcc ccgtggaatt gttagatcag
      721 gttttcgaac ttggtctggg tgctggcttc ggtgatgact ggctgcgcat tggttccgtc
      781 aaagttttca tggatggtgc acttggtcca catacggctg ccatgttcca gccctatatc
      841 ggcgaaggcg aaaatcgcgg cattttaaac atggacggtg aagaactttt cgaacatgga
      901 cgcaaagccg cgcaggtggg actgggaatg acagttcacg caattggcga ccgcgccaat
      961 catgaagtgc tcaatgctta tgaagcgtta cgcacctacg aaaaagaaaa ccatctccct
     1021 gcccttcggc atcgcatcga acatgtgcag gttattcacc cggatgatgc gccgcgcctg
     1081 ggcaggatga atatcattgc ttcgatgcag cccatccacg ccacatctga tatgctgatg
     1141 gcagatcaat tctggggcga acgctcaaga ctcgcctacg ctatgagaac tcaattggat
     1201 ttcggcgcgc ggctcgcgct cgggtcagac gcacccgtgg aatcaccgaa tccattttgg
     1261 ggattgcacg cggcagtcac gcgccagcgc gccgacgggt ctccgtcacc cgatggctgg
     1321 taccccgaac aaaaactgac catggctgaa gctgtcgcgg gttacacttc tgggccagct
     1381 tatacagcga acatggaaga tcgcctgggc aagcttgctg caaactatct ggcagatctg
     1441 attgtgctgg aaaaagatat cttcacctgc aatccaaatg atttgctcac gcttgaatca
     1501 tcggctacaa tgataggtgg agaatgggtc tgggaaaaac cctaaaggtt ttttcattga
     1561 tccatgaagg aatttggatt ttgggccgat gtgtcattgg ttggacaata ttgtgaacaa
     1621 gccagacctt tagggcttta ttactaacat ggcagaacaa ccacgcctca cacctgaaat
     1681 gctggtccct cgcctcgggg aatatttgat tcaaaaagga ctcattgaca ccgcgagctt
     1741 gcagaaggcg ctcgattatc aacaggaaga agtttcaaag gggaactcag tgatgttagg
     1801 gcaggccttg attgaccagc agctcatcga tcgcgctgcg ctggatcagg ccgtcaccga
     1861 gcagatcctg cagctacgct ctgcgctgca gtctgcaaat cgaactttgg aacgccgcgt
     1921 agaagaacgc accgcagaat tgcaaaaagc gctggagcgc gtctctgaac tgagccagct
     1981 gaaagccaat ttcatttcca acatctccca tgagttacga accccgctca cacacgtcaa
     2041 aggctatgtc gagttattga ttacaggctc catgggtcca attacagacg aacaacgtca
     2101 cgccttacag gtcagccagc aatcaaccct caggcttgaa atgctgatcg aggaccttat
     2161 catggtctcc cttgcctcac gtggtgaact aagcatcaac cagcgagacg tagatattcg
     2221 ccggctggcg aatctcgcag ccaagtcttc gtcagaaaag gcacagcaac gaggagtgaa
     2281 tctgcatgca gtgattgacg gcaatctgcc tcttgtgcag gcagattcgc agaagattgc
     2341 atgggtgttg aatcaactga ttgataatgg gatcaagttc accccggcgg gcggaagtgt
     2401 ggtggtcagc gtccagcgtg aaggggaaaa cctcgtgctc gtttctgtta cggataccgg
     2461 gatcggcata ccgatcaaca agatgaagga catctttgag ccattccacc aactggatgg
     2521 ttcatccaca cgccgttatg gtggaacagg gcttggtctt tcacttgtac gcgatattat
     2581 cgaagcgcat ggttcaatgc tggatgtgca gtccacagag ggacgcggga ctacatttaa
     2641 attcccattg cttatcacag taaattaaaa taaacctatg gattacaaaa ccctcgagta
     2701 cctgcaggaa ctcatgcgca cgacagcttt tgcagaaaac tcgcgcctgg tcgttgagaa
     2761 ttttctcaaa acattgcgtg aacagtttgt gtttgataac gtcgcggtct atgtctatga
     2821 tgattcaacg caaagcctgg atgtggtcta tgcccgcgct gttggacgag ctaaaactgc
     2881 tgaagctgat gcagcctggg gagaaacatt cgctggtcag gtactcaaaa gcgggaaatt
     2941 gctgcttcaa gatccaaagg cggatgcgcc gctggatgac cgtttaagcc gtgcctatct
     3001 gcttggtttg cctcttcgtt cagacatagc catcaaaggt gcgctgattt ttgtacgttt
     3061 tggcggtccc gtttatgaaa gtgaacatat cacactggct tcctttgcgg cggatctgat
     3121 ttcaaacttg ttcgagcggg cggcatggaa agaaaccatt tcagaattaa gcgaactaaa
     3181 acgccagatg caactgcagg aagattttgt c
//
LOCUS       DASTJP010000004         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1266467, whole genome shotgun sequence.
ACCESSION   DASTJP010000004 DASTJP010000000
VERSION     DASTJP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266467"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="VFQ23_00055"
     CDS             complement(<1..2026)
                     /locus_tag="VFQ23_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9904988.1"
                     /translation="MSTWIGKTLGKVYIESLIARGGMAVVYLGKHLTLQREVVVKILR
                     NDYEDDVASLDRFQREARVVAKLRHPNIVQIFDFDTVEDQSYIVMEYVNGPSLSRYLN
                     PLHKKLGRLELPTVSRVLTGVAHALQYAHESGVIHRDVKPANILLASRSSSIVLGELL
                     PPDFEPILTDFGLVRFLSTSMQTSAGKIVGTPAYMSPEQALGEHTDERTDIYSLGIVL
                     YEMLAGHVPFDGETTMGILLKHINERPPPIPGLAFGFQYVLDRALEKKPEKRFQTPGE
                     LAAAFNEVLEETSGASTLVKLTPRPKKKSREVKWQWKWIPAVLAIILIGMLSTIFFRN
                     NAFSPTSIPSTETPTASPTLEAPATPTTAPLPLGPTGLVRFQDGNAILDQINITARAM
                     PAPPEGNQYEAWLVSAVVDERLSLGLLEIDENGQGTLTYTDAENRNLLALYDGVEIRI
                     KPDADADINGVEQVAYSYTLPNAGLQYARQLMVSISILPQQVALIHGISANSQLMSKT
                     GEELLSAYENGDEAGVQENAEAMMNLMVGSKSAQHKDWNGDGQISDPGDGYGFLINGD
                     NLGYIQALYSYADYVANSPGASLNMISNGENLKVCAQNLSQWTAQLRDHAQTILTSDS
                     TDVASPVRDSAAMAVRILNGIDEDNDGVIEAVSGECGVLSSYEFAYRMLDM"
     gene            2025..2156
                     /locus_tag="VFQ23_00060"
     CDS             2025..2156
                     /locus_tag="VFQ23_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9904989.1"
                     /translation="MYKVLRVASENPFGELYGLIMWMAAGSVNTGIPYFGLQMDCIC"
     gene            complement(2203..4011)
                     /gene="metG"
                     /locus_tag="VFQ23_00065"
     CDS             complement(2203..4011)
                     /gene="metG"
                     /locus_tag="VFQ23_00065"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET9904990.1"
                     /translation="MPENILIAIAWPYANAEIHVGNITGSHLPGDIVARYHRLKGNKV
                     LVVSGTDSHGTPVTLAADKEGKPVEEVYKEYHDGFLELFQGFGITYDLFTTTHTENHF
                     KVTQAIFLALKENGFLYTATSKQWYSPSGNRFLPDRYVEGTCYICGFEGARSDQCDNC
                     GNVLEPERLINPRSKEGSALELRETEHFYLDLSKLEPDVKKFLEARASHMRDTVLGES
                     LRKIEAEGLRPRPITRDLDWGVPIPIDGWTEAGKRIYVWFEAVIGYLSAPIEWSQLAG
                     EPEAWREWWVNPNAKQFHFIGKDNIFFHTSLWPAQLMGAGSQFLKIFNGDDKPLTVPF
                     DVPANQFMNLEGQKISGSRNWAVWGRDALTRYDPDALRYYLTVNMPENKDSDWDWAEF
                     VARNNNELVATWGNLANRVLSFTYKHWEGHVPEVDPGALREIDQNLLATVENGFNTVG
                     ADLEAVHLRSALGEAMKLATAVNQYLDQTAPWSAIKTDHDAAAKTIYTALRAIDSLKV
                     IFAPFLPFTCERLHTFFGYETPIFGEQYTEEVTDSLGTHRVLRYRPIALSGAEGAVEA
                     WKPSELKPGAKLNQPAPLFKKLEESVIEEERARLGN"
BASE COUNT          967 a         1087 c         1059 g         1083 t
ORIGIN      
        1 gcatatcgag cattcgatat gcaaattcgt aggatgatag cactccgcat tcgcctgaga
       61 cggcttctat tacgccatca ttatcctcat caattccgtt caggatgcgc actgccatgg
      121 ctgctgaatc gcgaaccggc gacgcgacgt ctgtagaatc ggatgtaaga attgtctgag
      181 cgtgatcacg cagttgcgca gtccactgcg acaaattctg tgcacagacc tttaggtttt
      241 ctccattgct gatcatattc agggatgcac ccggagaatt ggcgacatag tctgcatagg
      301 aatacagcgc ctggatgtag cccagattat ctccgttgat taagaatccg tacccatcac
      361 ctggatctga tatctgccca tctccattcc aatctttgtg ctgcgcgctt ttactaccga
      421 ccatgagatt catcatcgct tcagcatttt cctggactcc agcctcgtct ccattttcgt
      481 atgcactcaa cagttcttcg ccagttttac tcatcaactg gctatttgcg gagattccgt
      541 ggatcagcgc gacctgctgc ggaaggattg aaattgacac catcaactgg cgtgcgtact
      601 gcaatccagc gttcggcagc gtgtaagaat acgcaacttg ttccactcca tttatatctg
      661 cgtcagcatc aggcttaatt ctaatttcta cgccatcgta aagcgccagc aaattgcgat
      721 tttcggcatc ggtataagtc agagtccctt ggccgttttc atcaatctcc aacaagccca
      781 ggctcaggcg ttcgtccaca actgcactta ccaaccaggc ttcatattga ttcccctcag
      841 gcggcgcagg cattgccctg gccgtgatgt tgatctgatc cagaatcgca ttcccatctt
      901 gaaagcgaac gagaccggtt gggccaaggg gcaaaggagc cgtcgtaggt gtggcgggcg
      961 cctcaagcgt cggagatgct gttggtgttt cggtactcgg gattgatgta ggtgaaaaag
     1021 cgttgtttcg aaaaaagatt gtactgagca taccgatcag gattattgcc aagaccgctg
     1081 ggatccactt ccactgccat ttaacttcgc ggcttttctt cttcgggcgc ggtgttaact
     1141 tcacaagagt actggcgcca gatgtttctt ccaacacttc attgaaggcg gcagccagtt
     1201 cacccggcgt ttggaagcgc ttctcaggtt ttttctccag cgcgcggtcc agtacatatt
     1261 ggaatccaaa ggctaaaccg ggaatcggtg gaggacgttc attaatgtgc ttgagcagga
     1321 ttcccatcgt agtttcccca tcgaagggaa catgcccagc cagcatttcg taaagcacga
     1381 tacccagcga atagatgtct gttcgttcat cggtatgttc accgagagcc tgttcgggac
     1441 tcatataggc aggcgtaccg acgatctttc ctgcagaagt ttgcatgctc gtacttaaga
     1501 agcggaccag gccgaagtcg gtaagaattg gctcaaaatc tggtggaagc aattccccca
     1561 gaacgattga actcgatctt gaggcgagta ggatattcgc cggttttaca tccctatgga
     1621 tcacgccgct ttcatgagca tactgaaggg catgcgctac gcccgtcaaa acgcggctga
     1681 ctgtgggaag ttccagccgt ccaagcttct tatgaagtgg gttcaagtat cgcgagagcg
     1741 atggaccatt gacatattcc atcacaatgt aagactgatc ctcgacggta tcaaagtcga
     1801 atatttgaac aatgttcgga tggcgtaatt tcgcgacaac gcgcgcttcc cgctgaaatc
     1861 gatccaggga ggcgacgtca tcttcgtagt cattgcgaag aatttttaca accacttcgc
     1921 gttgcaatgt gagatgcttc cccaaataaa ccactgccat acccccgcgc gcgatcagtg
     1981 attcaatgta gactttccct aatgtttttc cgatccaggt gctcatgtat aaagttcttc
     2041 gcgtcgcttc cgagaatcca tttggtgagt tgtatggact tattatgtgg atggcagcgg
     2101 gtagtgtcaa tacgggcatc ccctattttg ggttacaaat ggattgcatt tgttaagcaa
     2161 ataaaaaaaa ctcggatgtc aattcgacat ccgagtcaag acttagtttc ctaaccttgc
     2221 cctttcctct tcgatcacgc tttcttccag cttcttaaag aggggagccg gctggttgag
     2281 tttggctcca ggtttcaact cgctgggttt ccacgcttcg actgcgccct cggctccgct
     2341 cagcgcgata gggcgatatc tcaacaccct atgcgtcccg agcgagtctg tcacctcttc
     2401 ggtgtattgc tcaccaaaaa tcggagtttc atagccgaag aacgtatgca atcgctcgca
     2461 cgtgaatggc aggaacgggg cgaagatcac tttgagcgag tcaattgccc tgagcgcagt
     2521 atagatagtt tttgccgcgg cgtcatggtc agttttgatc gctgaccagg gagcggtctg
     2581 gtccaggtat tggttcaccg cggtggcgag cttcattgct tcacccaggg cggaacgcaa
     2641 gtgtacggct tccaggtctg caccaacagt gttgaatcca ttttccaccg tggcgagaag
     2701 attttgatca atttcacgaa gcgcaccggg atcaacctcc ggtacgtgcc cctcccaatg
     2761 tttataagta aaggacagca cgcggtttgc aaggttgccc caggtagcga ccagctcgtt
     2821 gttgttgcgc gccacgaatt cagcccaatc ccagtcgctg tccttgtttt cgggcatatt
     2881 gacagtcaaa tagtagcgga gagcatccgg gtcgtaacgc gtgagggcat ctcgtcccca
     2941 tacggcccaa ttgcgagagc cactgatctt ctgcccttcc aggttcatga actggttggc
     3001 gggcacgtca aatggaacag tcagtggctt atcgtcgcca ttgaagatct tgaggaactg
     3061 actgcctgcc cccataagct gagcgggcca cagcgatgtg tgaaagaaga tattgtcctt
     3121 gccgatgaag tgaaattgct ttgcgttggg attgacccac cactcccgcc acgcctccgg
     3181 ctcacccgct aactgactcc actcaattgg tgcggagaga tagccgatga ctgcctcaaa
     3241 ccaaacatag atgcgtttgc cggcttcggt ccagccatca atgggaatgg gcacacccca
     3301 gtcgaggtca cgggtgatcg gtcgaggtct tagtccctcc gcttcaattt tgcgcagaga
     3361 ttcgccgagg actgtatctc gcatatgcga tgcacgtgct tccaggaact tctttacatc
     3421 gggttcgagt tttgaaagat cgaggtaaaa gtgttcggtc tcgcgcaatt cgagggcgct
     3481 gccttccttg gagcgggggt tgatcaacct ttcaggttca agcacattgc cgcagttgtc
     3541 acattgatcg gagcgcgcac cttcaaatcc acagatatag caggtgccct cgacgtaacg
     3601 gtcaggcagg aagcgatttc cagacgggga gtaccattgt ttcgacgtag cggtgtagag
     3661 aaagccattc tccttcaagg cgagaaaaat cgcttgtgtg actttaaagt ggttttcagt
     3721 gtgagttgtc gtgaacagat cgtatgtaat gccaaagccc tggaagagtt caaggaaacc
     3781 atcatgatac tctttataga cttcctcaac cggtttgcct tccttatctg cggcgagcgt
     3841 gacaggcgtc ccatgcgagt ccgttcccga taccaccagc actttgtttc ctttcagtct
     3901 gtggtagcgc gcgacgatgt cacccggaag gtgtgagccg gtaatatttc cgacatgtat
     3961 ttcagcattg gcatagggcc aggcaattgc aattaagatg ttttcaggca ttcatgcctc
     4021 cgaattttat gttgtagggg caggcggcgc cttcccctac gagaggtcta aataaacaaa
     4081 aaggctgtcc taaacttgga cagcccgtcg acggtcagtt acgacgatct aacgtgctct
     4141 ccaggcgcga ggtaatttat ccatgcccat aggcattgac ctcaaaattg tcgctg
//
LOCUS       DASTJP010000005         6801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_843993, whole genome shotgun sequence.
ACCESSION   DASTJP010000005 DASTJP010000000
VERSION     DASTJP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843993"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="VFQ23_00070"
     CDS             <1..1766
                     /locus_tag="VFQ23_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET9904991.1"
                     /translation="PQPLVERRFRFGVPERIAANGEVLRPLDAAAVESLCKTLKAEGI
                     ESVAICLLHSFTNPRHELDIFEIVRDELPNVTISISHQVSPAMREYQRTSTTVANAYV
                     QPITSNYLDRIQNGLSGAGLDAPLNVMLSSGGMTTIETATRFPIRLVESGPSGGALAG
                     VFWGRQNGFQDILAFDMGGTTAKAILTKDSDLNITSQSEVAHVHRFKRGSGLPLLVPT
                     IEMIEIGAGGGSIAHINNLGLPAVGPESASSIPGPACYDQGGVQPTVTDADLMIGYLN
                     PNYFADGTIPLNVSAAEESFRDIADQLNISIPKAAWGIHQLVNENMAAAARVHAAERG
                     LDIHKYAMVTTGGAGPVHACGVAERLNIQTVIVPPIAGVGSSFGFLLAPISFDFTRSY
                     LTRIDQVDTSHLMKILDDLEREGREIVMKAGVTAGEIRVTRSVDMRYLRQGYEIRIPL
                     EDGTITSESLSALECRFEEEYLRFYGLLCEGVPIQAVNWRVVVSGPALEIDGKALKVG
                     EPLPKVNQPYGSRRVVFQPDDGDVDTPVYRRDSLSASFQTDGPVIIEEAESTTIVLPG
                     WSVAVQENSCLLLKRNGRDSR"
     gene            1763..3472
                     /locus_tag="VFQ23_00075"
     CDS             1763..3472
                     /locus_tag="VFQ23_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET9904992.1"
                     /translation="MIDPILLEVLWKRLISIVNEQAAALIHSSFTTLVREAGDLSAGV
                     FDAQGRMLAQAVTGTPGHINTMANCVRDHVVPQFPIDTLHPGDTLITNDPWAASGHLF
                     DQTIVTPVFYKGKGIGWFASTCHAMDIGGLTMGGDATEVFEEGLAIPLMKLFKAGAAN
                     EDLFDIIRANVRVPDEVIGDIYAQQAGNVVGSRKLLEMMDEYKLDDLEELSNLVLDRT
                     EQSLRDAIKKIPNGIYQSAVTIDGFDDPVMIHCKVKVADTDIEVDYEGTSPQIRRGIN
                     VVMNYTHAYTTYTLMCIVAPDIPNNEGAFRPIKVKAPEGSILNCRRPAPVSARHLIGH
                     FVSQPILNALSNPIPNQVIADGSAGLWNTMFEGELNQTGEVFAYIYFSAGGMGARPYR
                     DGLSATAFPSGITGVPAEVIESVAPIRMHKRELITDSGGAGKFRGGLGQEMVLEVLTG
                     LPAIHSCMYDRTRFAANGYHGGLGGTLGEVVLSNGHHPHPKSRYIIQPRQTVTLRLPG
                     GGGFYSAIERDPASVLEDVKQGYISVTAARETYGVVLDETTMTVDELATRDVRTKKQS
                     QEQ"
     gene            3469..4851
                     /locus_tag="VFQ23_00080"
     CDS             3469..4851
                     /locus_tag="VFQ23_00080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9904993.1"
                     /translation="MSNSTTADVVICGAGIAGVSTAYQLAVKHDMKNVIIVDPLPPLT
                     MTSDKSMECYRNWWPGPGDEMVRLMNRSIDLMEELHQEASNRLAMNRRGYIYATANPK
                     NVDAMISSAQEITSLGAGELRIHRNRADDPEYVPGSKHGLFDAPTGADLFLDQSLIRT
                     YFPYVTERTIALLHTRRCGWFAAQQFGMYMFEQAVAHGVQHIKGRVEGVEVRSNRIQS
                     VKVRVNGGVQEISTRTFVIAAGPLQKEVGRMIGVDIPVLCEPHLKVMFNDLYRVIPRD
                     MGLFIWNDPVKLPWSDEERAALAESDETRWVLDELPVGVHGRPEGDGNMIILQWSYHD
                     SKSEFPVFPIPIDEQLPEVALRGMGTVIPGLAKYFSQLPKPFIDGGYYARTPENRPLI
                     GPLPVEGAFIIGGLGGFGMQVSCGASDLLAHHILQDDLPSYAPAFLLSRYDDPQYQEL
                     LSQWEASGQI"
     gene            4911..5738
                     /locus_tag="VFQ23_00085"
     CDS             4911..5738
                     /locus_tag="VFQ23_00085"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9904994.1"
                     /translation="MKLQNKIAVITGGAQGIGAAIAKRFAQEGASVVIADVNVEKSNT
                     LVSEIESIGGEAISLLCDVSNSGQVDEMFNGALERFGRLDVLVNNAALVHHPDSNKNF
                     LELSEKAWLRALDINLTGMFLCSQRAARIMVKQVREGASSGGAIINMSSGGGSRAHRH
                     LMAYDTTKGGIEAATRAMAVDLAPWKIRVNVVVPGNITVDNALGGATGPDAAARTIPL
                     GRPGSPSDVASAAAFLASDDAAYITGQRVVVDGGMDAQLRSPAVDVQIDLGIADRLK"
     gene            complement(5848..6027)
                     /locus_tag="VFQ23_00090"
     CDS             complement(5848..6027)
                     /locus_tag="VFQ23_00090"
                     /inference="COORDINATES: protein motif:HMM:NF018715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HET9904995.1"
                     /translation="MDLLQWALMAFVVAAIAGLFGFTGVAQGAASVGRTLFGLFLVIA
                     AVLLVLALLGVSILT"
     gene            complement(6194..>6801)
                     /locus_tag="VFQ23_00095"
     CDS             complement(6194..>6801)
                     /locus_tag="VFQ23_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9904996.1"
                     /translation="QIQKQVVFDLLNRQSGWIQYIKLLIMATILVAGIEVGFLMTPFG
                     VFAPNDLWGFILEILIIAPILVILAWWYLAYARFISLRIIASQIGPEFSVWSKRFLQM
                     ASGLWTFLFFIPGLFLSRFLEGELLEVFMQTSLIWLAATLLLSFMAFVMSGVIIKIFI
                     ENQPKKCPHCGKITKRINPALEQCEHCEGGLGEWLFVPEIS"
BASE COUNT         1610 a         1636 c         1899 g         1656 t
ORIGIN      
        1 aaccgcagcc gctcgtcgaa cgaaggttcc gatttggtgt cccagagcgt atcgctgcga
       61 atggggaggt attgcgtccg ttggatgctg cggccgtcga aagcctgtgc aagacgttga
      121 aagcggaagg cattgagtca gtcgcaattt gtctgcttca ctcctttacc aacccgaggc
      181 atgagcttga tatctttgag atcgtgcgag atgaattacc aaatgtcacg atttcgatct
      241 cgcatcaggt ctcgccggcc atgcgtgaat atcaacgaac ctccactact gttgcgaacg
      301 cctatgttca gccgatcaca tccaattatc ttgatcgcat ccaaaatggc ttatcaggag
      361 cagggctgga tgcgccgctc aacgtaatgc tttccagcgg tgggatgacg accattgaaa
      421 ctgccactcg tttcccgatc cgcctggtgg aatcaggtcc atccggtggt gcgctggcgg
      481 gcgtgttttg ggggaggcaa aatggctttc aggatattct ggcgtttgac atgggtggaa
      541 caaccgccaa ggcgatcctc acgaaagatt ccgacttgaa catcacttcg caatcggagg
      601 tggcgcacgt tcatcgtttc aagcgcggaa gtggattgcc cctgctggtg ccaaccatcg
      661 agatgatcga gatcggtgcg ggtggcggga gtattgccca tatcaacaac ctgggacttc
      721 cggcggtggg acccgaaagc gcgagttcaa taccggggcc ggcgtgctac gaccagggtg
      781 gggtccagcc aacggtcacc gacgcagact tgatgatcgg ttacctaaac ccgaattatt
      841 ttgcagatgg aactattccg ctgaatgttt cagcagccga agagtccttt agggacattg
      901 ccgatcagtt gaatatttca atcccaaaag cggcctgggg catccatcag ctggtgaacg
      961 agaacatggc ggcagccgcg cgcgtccacg cggctgagcg aggcttggat attcacaaat
     1021 acgcaatggt tactaccggc ggtgcaggtc ccgtccatgc ctgcggtgtc gcggaacggt
     1081 tgaacatcca gacggtcatc gtcccgccta ttgcaggggt cggctcgtcg tttggattcc
     1141 tgcttgcccc gatttcgttt gatttcacgc gaagctatct cactcgaatc gaccaagtgg
     1201 atacgtctca cctgatgaaa attctggatg atctcgaacg ggaggggcgt gagatcgtta
     1261 tgaaggccgg agttacagcc ggggagattc gagtcacccg aagtgtcgac atgcgttatt
     1321 tgcggcaggg ctatgaaatt cgtattcctt tggaggatgg cactatcaca tctgaatcgt
     1381 taagtgccct tgaatgccga ttcgaagagg agtaccttcg attttatgga ttgttgtgtg
     1441 agggtgtccc gattcaggca gtgaattggc gcgtggttgt ttctggacct gcgctggaga
     1501 ttgatggaaa ggcgctgaag gtgggtgaac cgttaccaaa agtgaatcag ccctacggga
     1561 gtcggagagt tgtttttcaa cccgatgatg gggatgtgga tacgccagtg tatcgtcgcg
     1621 actctctttc agcctccttt caaacagatg gaccagtgat catcgaggaa gccgaatcta
     1681 caacgattgt tttgccgggg tggtcagtgg cggttcaaga aaatagttgc ctcttgctca
     1741 aacggaacgg aagggattcg cgatgattga cccgattttg ctcgaagtgc tgtggaagcg
     1801 cttgatttca attgtgaacg aacaggctgc cgcgttgatc cattcgtcgt tcacgacgct
     1861 ggtgcgcgag gcgggcgatc tatcggccgg agtgttcgac gcacagggac gaatgctggc
     1921 gcaggctgtc acgggcacac cagggcatat caacacgatg gccaattgtg tgcgtgatca
     1981 tgttgtgccg caatttccca ttgacacgct ccacccaggc gatacgttga ttaccaacga
     2041 cccgtgggcc gcctcggggc atttgtttga tcagaccatc gtcacgcctg ttttttacaa
     2101 ggggaaaggc attggctggt ttgcaagtac atgtcacgcg atggatatcg gtgggctgac
     2161 catgggtggg gatgcaaccg aagtgtttga agaaggtctc gcgattcctt taatgaagtt
     2221 attcaaggcg ggcgccgcga atgaagatct gttcgatatc atccgcgcca atgtgcgcgt
     2281 tccagatgaa gtgatcggtg atatctatgc ccagcaggca ggaaatgtgg tggggagtcg
     2341 caaactgctc gagatgatgg atgaatataa gctggatgat ctcgaagaat tatcgaatct
     2401 agtgctggat cggacagaac aaagcctgcg cgatgcaatt aaaaaaatcc ctaatggaat
     2461 ttatcaaagt gcagtcacga tagacggatt tgatgatccg gtcatgatcc actgtaaggt
     2521 taaggtggct gataccgata tagaagttga ctatgagggg acctcgcccc agattcggcg
     2581 cggcatcaat gtggtgatga actacactca cgcgtacacg acgtacacgt tgatgtgcat
     2641 cgtcgcaccg gatatcccga ataatgaggg cgcgtttcgt ccgatcaaag tgaaggcacc
     2701 cgaaggttcc atattaaatt gccgccgacc cgcacccgtc agcgcgcgtc atttgatcgg
     2761 ccatttcgtt tcgcagccga ttctcaacgc gctatcgaat ccgattccaa atcaggtcat
     2821 cgcggatggt tcggctggat tatggaatac gatgtttgag ggtgagttga atcaaactgg
     2881 tgaggtcttc gcctatatct atttttcagc aggcggcatg ggggcgcgtc cgtatcgcga
     2941 cggtctttct gccaccgcgt ttccaagtgg catcactggc gtcccggcgg aagtgatcga
     3001 atccgttgcg ccgatccgga tgcataaacg cgaattgatc accgattcag gcggcgcggg
     3061 gaagttccgc ggtgggctgg gccaggagat ggtgctcgaa gtgcttaccg gtttaccggc
     3121 tatccattcc tgcatgtacg atcgtacgcg tttcgcggcc aatgggtatc acggtgggtt
     3181 gggggggact cttggtgaag tagtgctctc caatggacat catccacatc ccaagagtcg
     3241 atatataatt cagccccggc aaaccgtcac tctgcgcctg cccggtggcg gtggatttta
     3301 ctctgccatc gaacgcgacc ctgcctcggt gctcgaggat gtgaagcagg gctacatctc
     3361 ggtgactgct gcccgggaaa catatggcgt ggtacttgat gaaacgacga tgacggttga
     3421 cgagctggct acgcgtgatg tgcgtacgaa aaaacaatca caggaacaat gagtaattcg
     3481 acgaccgcgg atgtcgtgat ctgtggtgct ggaatcgcgg gcgtttcaac ggcgtatcag
     3541 cttgctgtaa agcatgacat gaaaaatgtc atcatcgttg atcctttgcc gcccctgacg
     3601 atgacgagcg ataagtcgat ggagtgctat cgcaattggt ggccgggtcc cggtgatgag
     3661 atggtgcggt tgatgaatcg cagtattgat ttaatggagg agcttcatca ggaagcatcc
     3721 aaccgcctgg ccatgaatcg acgcggttac atttacgcta cggcaaatcc caaaaatgtt
     3781 gatgcgatga tttcaagtgc acaggaaatt acaagcctgg gcgcgggtga gttacgcatc
     3841 catcgtaatc gcgcggatga tccggaatat gtccctggca gtaaacatgg cttgtttgac
     3901 gcgcccaccg gggcggatct tttcctcgat caatccttga tacgtactta ttttccgtac
     3961 gtgaccgaac gaacgattgc attgttgcac acgcgacgat gtggctggtt tgctgcccag
     4021 caattcggta tgtacatgtt tgaacaggca gttgcacatg gcgtgcaaca tatcaaggga
     4081 cgagtcgaag gcgttgaagt gaggagcaat agaatccaga gtgtcaaagt gagagtaaat
     4141 ggtggtgtcc aggaaatctc gactcgaacc tttgtgattg ccgctggtcc cttgcaaaaa
     4201 gaagtcggaa gaatgatcgg tgttgacatt ccagtgctgt gcgagccgca tctcaaggtg
     4261 atgttcaacg atctgtatcg agtcattcct cgcgatatgg gtttatttat ttggaatgac
     4321 ccggttaagt taccctggtc tgatgaggaa agggcagcgc tcgctgaatc tgatgagact
     4381 cgctgggtat tggatgaatt gcccgtcggt gtgcacggaa ggcccgaagg tgatgggaat
     4441 atgatcattc ttcaatggag ctaccatgac tcaaagtcgg aattcccagt cttcccgatt
     4501 ccgattgatg aacagctgcc ggaggtcgcg ttgcgcggca tggggacagt cattcccggg
     4561 ctcgccaagt atttcagtca acttccgaaa ccattcattg acggtggcta ctacgcgcgc
     4621 accccggaaa accggcccct gatcggacct ctgcccgtgg aaggagcgtt cattatcggc
     4681 ggactgggcg gattcggaat gcaggtctca tgtggtgctt cagacctgct ggcacatcac
     4741 attcttcaag acgatctacc ctcgtatgct cccgcctttc tcttgagccg atatgacgat
     4801 ccgcagtatc aggaattgct tagtcaatgg gaagcctcgg ggcaaatcta ataggacccg
     4861 aacaaattgc gcggtcccat ttgggaccga ttattgggaa ggattggctg atgaaattac
     4921 aaaataagat cgccgtgatt acgggcggtg cgcaggggat tggtgccgcg attgcgaaac
     4981 gttttgcaca ggaaggtgcc agcgttgtga tcgccgatgt gaacgtagaa aaatccaata
     5041 cactggtatc ggagatcgag tcgattggcg gagaagcgat atctctgctc tgcgatgtct
     5101 caaattccgg tcaagttgac gaaatgttca atggagcact tgaacggttt gggcggctcg
     5161 atgtcctggt aaacaatgcc gcccttgtgc atcatcctga ctccaacaaa aactttcttg
     5221 aattatccga gaaggcctgg ttgcgcgccc tggatattaa tttaaccggg atgtttcttt
     5281 gcagtcaacg ggccgcgcgg attatggtga aacaggttcg tgagggcgcc tccagcggcg
     5341 gggcgatcat caacatgagt tccggtggcg ggagtcgtgc acatcgtcac ctgatggcat
     5401 acgacaccac gaagggtggg attgaagcgg caactcgcgc catggcggtg gatctggcgc
     5461 cgtggaagat tcgagtcaat gtcgtggtgc ctggcaatat tactgtggat aatgccctgg
     5521 gcggtgcaac cggtccggac gcagccgcgc gaacgattcc gcttgggcga cccggttcac
     5581 cctcggacgt tgcttctgcc gccgcgtttc tggcttccga tgatgctgca tatatcacgg
     5641 ggcagcgcgt cgtggtggat ggtggcatgg atgcgcaact tcgttctccc gccgtggatg
     5701 tgcaaattga cctggggatt gcagaccgtt tgaagtagtg cacatatcgc tgtgaaatcc
     5761 cccgagtatt tgacaaagaa gaagccgatg ttacatcggc ttcttcttca atattcattt
     5821 ttgcattctc cgtgatgatt tttatggtta ggtcagaatg ctcacaccca ggagagccag
     5881 cactaacaac accgcggcaa ttaccaggaa tagaccaaat agcgttcttc ctacagaagc
     5941 tgcgccctgt gccacaccgg taaatccgaa cagcccagcg attgcggcaa ctacgaaggc
     6001 cattaatgcc cattgtaaaa ggtccatgtt aagctccttt cgtttgctct tttagagtaa
     6061 ctcgactatt tgaaatagtc tgatactcat actttacatt tgcgaccgga tttaaacaat
     6121 ggggaatatg cccatccgct aagggatttc ctgccctgtt ttcctccttc gtatagtcac
     6181 tgaagacgct gtcttagctg atttccggaa caaagagcca ttcgcccagc ccgccttcgc
     6241 agtgttcaca ttgctcaaga gccgggttta tacgttttgt gatctttcca caatggggac
     6301 atttttttgg ctgattttca atgaatattt ttatgattac gccactcatg acaaaggcca
     6361 tgaaactcag taagagggtg gcagccagcc aaatcaaact ggtctgcata aatacttcca
     6421 gtaactcgcc ctcgagaaat ctacttaaaa acaatccagg aatgaagaat aagaacgtcc
     6481 acaacccgga agccatttgt aaaaaccgtt tgctccagac cgaaaactct gggccaatct
     6541 gggatgcaat tatccgaagg gaaataaatc ttgcataggc gagataccac catgccagta
     6601 taaccaagat tggggcgata attagaattt caaggatgaa tccccacaga tcattggggg
     6661 caaaaacacc gaacggtgtc atcaagaaac caacctcaat cccggcaact aaaatcgtcg
     6721 ccattattaa caactttata tattgaatcc agccgctctg tctgtttagt aaatcgaaca
     6781 cgacttgttt ttgaatttgc g
//
LOCUS       DASTJP010000006         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_949217, whole genome shotgun sequence.
ACCESSION   DASTJP010000006 DASTJP010000000
VERSION     DASTJP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949217"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFQ23_00100"
     CDS             <1..221
                     /locus_tag="VFQ23_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HET9904997.1"
                     /translation="KRLEKLKTWRKNVAQKMSVESDIVLPKRYLTVLAENPPKSLYEL
                     ESMMSDSPWRFSQYGNQILNLLNGGTRV"
     gene            250..1644
                     /locus_tag="VFQ23_00105"
     CDS             250..1644
                     /locus_tag="VFQ23_00105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9904998.1"
                     /translation="MQINFHGAAHTVTGSQHLLEINGNRLLLDCGMYQGRRAESYIRN
                     LNFAYDPSKVDAVILSHAHIDHCGNLPNLVKNGFQGPIYATHGTADLATIMMADSGRI
                     QESDANFVNKKRLARGEVPIQPLYTEADAERAALLFNPVDYGQPFVPVPGVVARFIEA
                     GHILGAGAVSLEIEENGKKTRLWFSGDIGRYDLPLLRDPVLPEAVDYMIMECTYGDKP
                     HNDPSEAFEEFKQTVSRTLKRGGKVIIPAFAVGRTQELIYHLNMMMFANDVPPAPVFV
                     DSPLAVNASQIFKKHPEYFDDETRWFVQQASHPALDFKLLTYVQSVEESKALNLRKDP
                     MIIISASGMAETGRILHHLRNNIENPKNTVCIVSWQAPHTLGRRLADREKQIKIFGEP
                     YTVMAEIATIGGLSGHAGQDLLTQYAVNVKDSVKKIFLVHGEAVPAAALTEKLKDHNM
                     REVYYPELHSSVEI"
     gene            1686..1772
                     /locus_tag="VFQ23_00110"
     tRNA            1686..1772
                     /locus_tag="VFQ23_00110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1720..1722,aa:Ser,seq:cga)
     gene            1833..3077
                     /locus_tag="VFQ23_00115"
     CDS             1833..3077
                     /locus_tag="VFQ23_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9904999.1"
                     /translation="MRTHWLLSIVILTVSTLLSGCGNLQVTESVPEQTSTPVTVGETP
                     TITAVPTITPTVESPPIDACKLQSRAFTNVGLGLPKPSHKLPTVGTVKTIVLFADFAD
                     APASQTPEEIFSLISPQAETFIRDLSYGKMEYVLEPYFVWLRLGQPSARYGEGIRTYD
                     GHLEFIQEAVNLADANVDFSEADSVLVMVPPQVSAVPYGPAFGANALAGYVADDKTFE
                     NGVTSGADLPGWGFLWLNHETGHIMSLPDLYAYDYDPSVYEDQHRFVGGFGLMGFIAG
                     KAPEFFAFERWQLGWLDDEQIFCQQTVEEIVTLSAVETPGGIKAIIVPINDSQAVIVE
                     SRRALGYDKDMLKPGALVYTVNTDIFSGEGPIVVYPALENDPYRDQSPLAVNESVTVD
                     GVTITVLESTETGDTVRVRVEQ"
     gene            3264..4115
                     /locus_tag="VFQ23_00120"
     CDS             3264..4115
                     /locus_tag="VFQ23_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET9905000.1"
                     /translation="MRLNLALAQIATTLGDVEANLEKHLEYIKQAKSQKTDLLVFPEL
                     SLTGYVLQDLVATVAHRPTEDDPVFKHLLKASRDLDLVIGFVDEDDRHRFFIASAYLS
                     GGRVLHVHHKVYLPTYGLFDEGRFFAWGDSIRSFDTRFGRVGLLICEDFWHASPPYLL
                     WLDGADIMLFSSASPGRGLNDREKLESARWVERVSKAYASMFTSFVAHCNRVGFEDGL
                     LFWGGATVNDPNGELIAHGPYFEEALTFAELDINQLRRTRARLPLLRDERTALVQKEL
                     DRILSRG"
     gene            4108..4929
                     /locus_tag="VFQ23_00125"
     CDS             4108..4929
                     /locus_tag="VFQ23_00125"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET9905001.1"
                     /translation="MADLTINTDVARQILTKFLRSEVTRVGYKRAVIGLSGGVDSALS
                     FALAVEALGPENVLAVRMPYQSSSADSLEHAKLLIDQFKVPSETIEITGIVEPLIERD
                     PQISKQRKGNIMARSRMIVLYDQSEVFKGLVIGTSNKTEILLGYSTLWGDMASALNPI
                     GDLYKTQVRQLSHALEIPSVIVDKAPSADLWAGQTDESELGFTYEDVDKLLYLLVDQR
                     YSQQECVEEGFDASFVNTVVMRIRRNQFKRMMPPVAKLSNRTIGYDFLYLRDWGT"
BASE COUNT         1287 a         1186 c         1347 g         1353 t
ORIGIN      
        1 tgaaacgttt ggaaaagctt aaaacctggc gcaaaaatgt tgcacagaaa atgagcgttg
       61 agtccgatat cgttcttcca aagagatatc taactgtgtt ggcggaaaat ccgccgaaaa
      121 gtctttatga actggaatct atgatgtcag attcgccctg gcgttttagc cagtatggaa
      181 atcagatctt aaatctatta aacggaggca cgcgggtatg aaggaagcat acgacggtag
      241 gagcctggga tgcagataaa ttttcatggt gcggcacaca cagtgacagg gagtcagcat
      301 ctgctggaga tcaacgggaa taggctcctg cttgactgtg gcatgtacca gggtcgccgc
      361 gccgagtcgt acattcgaaa tctgaatttt gcttatgatc cctccaaagt ggatgcggta
      421 atcctttcgc acgcgcatat tgatcattgt ggcaatttac ctaacttggt caaaaatggc
      481 ttccagggac cgatttacgc aacgcacggg acagcagacc tggctacgat tatgatggca
      541 gattccggcc gaatccagga gtcggatgct aatttcgtaa ataagaaacg acttgcgcgt
      601 ggcgaagtcc caattcaacc actttataca gaggcagatg cagagcgcgc cgcgttgcta
      661 ttcaatcctg tagattatgg tcaaccattt gttcctgtgc ccggtgtggt tgctcgcttt
      721 attgaggccg gtcatatttt gggtgctgga gctgtttctt tggagatcga agaaaacggt
      781 aaaaagaccc gcttgtggtt ttccggtgac atcggccgct atgatcttcc attgctgcga
      841 gatccggttc tcccagaggc cgtggactat atgatcatgg aatgtacata tggagataaa
      901 cctcataatg acccaagcga ggcatttgag gagtttaagc agaccgtttc acgtacatta
      961 aagcgaggcg gtaaggtaat aatcccggcc tttgcagtgg gacggacgca ggaattgatc
     1021 tatcatctga atatgatgat gttcgcgaat gatgttccgc ctgcgccagt ttttgtcgat
     1081 agccctcttg cagtcaatgc atcgcagata tttaaaaaac accctgagta ttttgatgat
     1141 gaaacccgct ggtttgttca gcaggcgagt catccggcgt tagattttaa acttcttacc
     1201 tatgttcagt ctgtcgaaga atcgaaggcg ctgaacctgc gcaaagaccc gatgatcatt
     1261 atttccgctt cagggatggc agaaactggg cgtatcctgc atcaccttcg taacaatatc
     1321 gagaacccca agaacacggt ctgtatcgtc tcctggcagg ctcctcatac cctgggaagg
     1381 cgcctggctg accgggagaa acaaatcaag atcttcggtg agccctatac cgtaatggca
     1441 gagatagcga ccattggtgg gctatctggt cacgcgggtc aggacctatt aactcagtat
     1501 gccgttaatg tcaaagacag tgtgaagaaa attttcctcg tccatggtga agccgtgccg
     1561 gcagcggccc tcacagaaaa gctgaaagat cataatatgc gtgaggtgta ttacccggaa
     1621 ttgcattcta gtgtggaaat ctgagttata atctgcgccg ctgttcatag tagtgtgaaa
     1681 cctttgggaa ggtgccagag tggttgaatg ggacggtctc gaaaaccgtt gtgggcttcg
     1741 gtccaccgag ggttcgaatc cctcccttcc cgctgaaaat ttttaaattg gggtaattca
     1801 gttctcaact attgctctac gagaaaattc aaatgagaac gcattggtta ttatccattg
     1861 ttattttgac tgtttctact ctgctttcgg ggtgtggaaa tctgcaggta acagaatctg
     1921 ttcctgagca aacctcgacg cccgtgaccg ttggggaaac accgactatc actgccgttc
     1981 cgacaattac acctactgtt gaatcgcctc ccattgatgc ctgtaaacta cagtcacggg
     2041 catttacgaa cgtcgggctc ggcctcccaa aaccatccca taaacttccg acggttggaa
     2101 ccgtgaaaac gatcgtgctt tttgcagatt ttgcggacgc gcctgcctca caaacaccgg
     2161 aagagatatt ctctctgatt tctccacaag ccgaaacttt tattcgtgat ttgtcttatg
     2221 gcaaaatgga atacgtgctt gagccatact tcgtttggct tcggttgggg caaccctctg
     2281 ctcgctacgg ggaagggata agaacctatg atgggcacct ggaattcata caggaagcag
     2341 tgaacctggc ggatgcaaac gtggattttt ccgaagcaga ttctgtcctt gtgatggtcc
     2401 ctccacaagt gagcgccgtg ccatacggac ctgcctttgg ggcgaatgcg ttggcaggat
     2461 acgtggctga tgacaagacc tttgaaaatg gagtaacgtc aggagctgat ctgccaggct
     2521 ggggatttct ctggttgaat catgagaccg gacatatcat gtcattgcca gacttatatg
     2581 cgtacgacta cgatccttca gtttatgaag atcagcatcg atttgtgggt ggtttcggtt
     2641 taatgggctt tattgccggc aaagccccgg aattctttgc atttgaacgt tggcagctcg
     2701 gctggctgga cgatgaacaa atcttttgtc aacagacagt agaggagatt gtgaccttgt
     2761 ctgccgttga aacaccgggc ggtatcaaag ccatcatcgt tccgataaac gattctcaag
     2821 ccgtcatcgt ggagtcaagg cgtgcgctcg gatacgataa agatatgttg aaaccagggg
     2881 cgctggtgta tacggtgaac acagatattt tttctggtga gggtccgatc gtcgtttatc
     2941 ctgctttaga gaatgacccc tatcgcgacc agtctcctct cgccgttaat gaatcagtca
     3001 ccgtggacgg agttacgatt accgtgttag agtcaacaga gaccggcgac acagtgcgag
     3061 taagagtcga gcaatagcat ctttcatcga ggtggacaaa cccgcgagac tcacatttga
     3121 gatgcggatg cctggcttga atgcagaatt aagaaaatca tttgcgatct cgttggtgaa
     3181 acgacaaaag aattgcatca cgtattggaa gccagagact aacttttagc aagtttccat
     3241 tgtttggtgg taagattcgt gtaatgagac tcaacctcgc cctcgcacaa atcgccacaa
     3301 cactggggga tgtcgaagcc aacctcgaaa aacatctcga atacatcaag caagccaaat
     3361 ctcaaaaaac tgaccttctg gtatttcctg aattgtcttt gacaggctat gtcttacagg
     3421 acctggtcgc caccgttgcc caccgcccaa cggaggatga tcctgtattc aagcatttgc
     3481 tcaaagccag tcgcgacctt gacctggtga tcggattcgt ggacgaagat gatcggcatc
     3541 gctttttcat cgcctccgcg tatctctcag gcggacgcgt tcttcacgtt caccataagg
     3601 tctatttgcc tacctacggc ctgtttgatg agggccgttt ctttgcgtgg ggcgattcga
     3661 tccgttcgtt cgacacgcgc tttggacgcg tcggactctt gatatgcgag gacttttggc
     3721 atgcctcgcc tccgtatctg ctctggttag acggagcaga catcatgcta ttttcatcgg
     3781 cgtcgccggg gcgaggcttg aatgatcgag aaaaacttga gtcagcccgc tgggttgagc
     3841 gggtaagcaa ggcctatgca agtatgttca cgtcatttgt tgctcattgc aaccgggtgg
     3901 gctttgagga tggattgctt ttctggggcg gggcgacagt caacgacccg aatggtgaat
     3961 tgatagccca tggaccttat tttgaagagg cactgacctt cgcagaattg gacatcaatc
     4021 aactccgtcg gacacgcgcg cggttgcctt tgcttcgtga tgagcgaacg gctctggtac
     4081 aaaaggagct ggataggatc ctttctcgtg gctgatctaa ccatcaatac cgacgtcgct
     4141 cgtcaaatcc tgacaaaatt cctcaggagc gaagtcacgc gtgtgggcta caagcgcgcc
     4201 gttatcggtc tttctggtgg agttgactcg gctctctcgt ttgcgttggc tgttgaggcg
     4261 ctgggccctg aaaatgtcct ggctgtgagg atgccatatc aatcatcatc ggctgattcg
     4321 ttggaacatg ccaaattgct gattgaccag ttcaaagtcc cgagcgagac gattgaaatc
     4381 actggaatcg tcgaaccact cattgagcgc gatccacaga tttccaagca gcgaaaaggc
     4441 aacatcatgg cgcggtctcg aatgattgtc ttatatgatc aatctgaagt attcaaaggc
     4501 ctggtgattg gcacaagtaa taaaaccgag attttgttgg gctacagcac gttatgggga
     4561 gatatggcct ccgccctgaa tccaattgga gacttataca agacccaggt gcgtcagctt
     4621 tcgcacgcgc ttgaaatccc ctccgtcatt gtggataaag cgccatctgc cgacttgtgg
     4681 gccgggcaaa cggacgaaag cgaacttggc tttacgtatg aggatgtgga taagttactt
     4741 tacctgctcg tcgatcaacg ttacagtcag caggaatgtg tagaagaagg gtttgatgca
     4801 agttttgtaa atacagtcgt catgcgcatt cgccgtaatc aattcaagcg tatgatgccg
     4861 ccggtggcca aattgagcaa tcgcaccata ggttacgatt ttctatattt gagggactgg
     4921 ggtacataat agagtttgac tcttaaaatt gcaaattttt ggagcagaag gttataacct
     4981 tctgctctta attgacggaa aggattgttt ggaatgattc gaatacctcc cgcgttaaag
     5041 tatcgcggtt ttgcgttgat ctggagcggg ttaatcatct caatggtcgg tacacagatg
     5101 cagcagtggg cactgttctg gcatatcagt caactcagta aagacccgat cgcggtgagc
     5161 attgtcggtg ggg
//
LOCUS       DASTJP010000007         5063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1582537, whole genome shotgun sequence.
ACCESSION   DASTJP010000007 DASTJP010000000
VERSION     DASTJP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582537"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1064)
                     /locus_tag="VFQ23_00130"
     CDS             complement(270..1064)
                     /locus_tag="VFQ23_00130"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET9905002.1"
                     /translation="MNENIEELFTFYALGAVTESERQQVEAYVASDPAARARLDEMIR
                     TASALSYASEPFEPPAALKQTLMDRVHADAQKRFAPPPPAQESGRSSFMNVFLSGQWF
                     PQAVAVASLLIAVAVGSWGLSLRNQLVRLQADMVSLQRELADQRVVLAHVVSPNSQTF
                     LISGTDHQPGARGQLMADTQTGSAVLVVSGLQQLDVGKIYEFWLIDGDTPIAAGLFEV
                     NEEGKAILQVSHAVTPESYDAIGVSIEPQAGSEQPTGAIVMLGALD"
     gene            complement(1070..1666)
                     /locus_tag="VFQ23_00135"
     CDS             complement(1070..1666)
                     /locus_tag="VFQ23_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9905003.1"
                     /translation="MQVLSKPIMIVQEWSDEQLIAQVARGDTAAYEMLYDRYSSAVMG
                     LALKITGDRSLAEEVVQETFWRVWRKAELFRTQRGAFTSWFFGITRNLSIDVLRRQKT
                     QIQPVEETEQIIEQAIDPSMNVPEAAWLREKQQQMRAAIETLPVEQRSVIEMAYFRGL
                     TRQEIAQATGEPLGTIHTRARLALQKLREELQMQGFEE"
     gene            1772..2239
                     /locus_tag="VFQ23_00140"
     CDS             1772..2239
                     /locus_tag="VFQ23_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9905004.1"
                     /translation="MTQVLYGPRLGREGRLRCGCSAIIFDESRTRVLLTRRADNGLWC
                     APGGAMESGESAAEACEREVFEETGLTVRVQRLVGVYSNPDQLVIYPDGTKVHIVAMS
                     FEAQVITGELVLTNETTSVGYFTFEEIDTMDFFGSHKQRIYDALENQKTAFLR"
     gene            2292..4112
                     /locus_tag="VFQ23_00145"
     CDS             2292..4112
                     /locus_tag="VFQ23_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET9905005.1"
                     /translation="MFGGIETDQLFLFFILIGSIVLLFTEWIRIDLTAVLIILMLSLT
                     GVLEPEAAFSGFSSEPAILLASVFVLNGALYHTGLSEQLGNRIKQWAGKSFEQTIGVV
                     MPSVALLSAFTHHVTITALMLPPTLKLARENNIPASKLLIPLSFAASLGTAITIIGAP
                     AFLIADGLLKQARQPGLGIFSIAPIGLALTLAGTIFFLLLGRFLLPARRGDEEAIDHF
                     RLEGYSTELVILPDSPFIGKTIRQIEEEQPTELKVSAWYRDDRPRSRPFGSKKAQAGD
                     VLVIRTNPDRLATIQQEPGIALRPLQKYEEAFSPTQDSDNSKEELASRLVQCVVAPRS
                     DLIGKTIGKVDFLQNYGVIVVSVWRRKGWLRTELSRVKLQEGDVLVMVGDNTAFKQIS
                     GDRSFLMLVPFKGEPKPRHKARLAGIIMLASIILATINVFPVQVPLLAGAVIVILSGC
                     ISMSQAYQSIDTRIYVFIAGAIPLGLAMQETGTAILLANWMEGLVSTWDIHWILLMLF
                     LITGLVTQVMSDSATTALFAPVAIALARSLDLPPEPLVVTVAMASVTSFFTPLGHHGN
                     LLIYGPGRYQFADFLRVGIPLTLIVAVIVSFMAPMIWPII"
     gene            4183..4746
                     /locus_tag="VFQ23_00150"
     CDS             4183..4746
                     /locus_tag="VFQ23_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905006.1"
                     /translation="MLNKISEKFHSWAKGRWVLVALIAYALMAGYVMPVAAGIIAFAA
                     NNSVLPLDLLFFYTPEQAYSMIDKYGEAGRSIYWKIELTADIIYPITYTLFFGLLLSW
                     LFQRGFKSNHQIQKWNVLPVGGWFFDMLENVGIVSMLSVYPAKPAFLAWLTMIFGSLK
                     WAFAAVSIVLILIGLVRAVINGFRKQE"
BASE COUNT         1190 a         1256 c         1252 g         1365 t
ORIGIN      
        1 tacggtatgc aacaagtgat gattgcctcg cacattaagg caataccatg aattacttta
       61 aggaattgtc ctgtagtgga acctacccgg cccttcgtcc cagagagaaa taattctcat
      121 cacttacaaa caatcaatac cgtgttctat aggtattacg tggggacatg aaatttgcat
      181 ttatgaaatt gagtcaatat tggaaaaaaa caaaagcctt ctgatacttt tatcagaagg
      241 cttttataga aaatttttat taaggtaatt tagtcgagtg cacctaacat tacgatagcg
      301 cccgtgggtt gttcactccc agcttgtggt tcgatggaga caccaatggc atcataggat
      361 tcaggtgtga ccgcatgaga aacctgcagg atggccttgc cctcttcgtt tacctcgaac
      421 aagccagcgg ctataggcgt atcgccatcg atcaaccaaa attcatagat ctttcccacg
      481 tccagttgtt gtaaacccga tacaaccagc accgccgaac cggtctgtgt atcggccatt
      541 aattggcccc gggctcctgg ttgatgatcg gtacccgaga tgaggaaggt ttgtgaattt
      601 ggtgacacaa catgtgccag caccactcgc tgatctgcca gctctcgttg aagggagacc
      661 atatccgctt gcaaacggac caattgattc ctcagagaca atccccagct accaaccgcc
      721 acggcaatta acaaacttgc caccgctact gcctgaggga accattgacc tgataagaac
      781 acgttcatga aactagaccg acccgactcc tgcgccggag gtggaggcgc aaatcgtttt
      841 tgagcatccg cgtggacacg atccatcaac gtttgtttca acgctgcagg cggttcaaat
      901 ggttctgagg cgtaggacaa cgcagaggcg gtgcgaatca tctcatcgag tcgcgcccgc
      961 gctgcagggt cagatgcgac gtaggcttcc acttgctgtc gctccgattc agtcactgcg
     1021 cccagtgcgt aaaaagtaaa aagttcttcg atgttttcgt tcatcctagc tattcttcga
     1081 atccttgcat ttgcagctct tcgcgcaact tctgcaaagc taatcgggcg cgggtgtgga
     1141 tcgtaccgag cggttcgccc gtggcttgag caatctcctg acgagtcagg ccgcgaaaat
     1201 aagccatctc gatcacactc cgctgttcta cgggcagagt ttcaatcgct gcccgcattt
     1261 gttgttgttt ttccctcagc caggccgctt cggggacatt catgctggga tctatggcct
     1321 gttcaatgat ttgttctgtt tcctccaccg gctgtatctg tgtcttctgg cgtctcaaca
     1381 catcaatcga aagattacga gtaatgccaa agaaccagct ggtgaacgca cctcgctgag
     1441 ttcggaatag ctcggccttc cgccagacgc gccagaaggt ctcctgaacg acttcttccg
     1501 ccagggatcg atcaccggtt attttcaggg ctaaacccat cactgctgag gaataacggt
     1561 cgtagagcat ttcatacgcg gcggtatcac cacgcgcgac ttgtgcgatc aattgctcgt
     1621 cactccattc ctggacaatc atgattggct tactcaagac ctgcattcat aatgatatac
     1681 gtgctggggg ctaaaatgaa tctatgatga ataaggagca ttatacttga caggcaggtc
     1741 ccattcgcaa aagaaaggct cttaaaaatc catgacacag gttctttacg gtccgcggct
     1801 tggcagggaa ggtcggctcc gctgtgggtg ttcagctatc atctttgacg agtcgcgtac
     1861 cagggttttg cttacacgcc gtgctgacaa cggcttgtgg tgtgctcctg gcggggcgat
     1921 ggaatcaggc gagtctgcgg cggaggcctg cgaaagagaa gtgtttgaag aaacaggttt
     1981 gacggtccgt gtgcaacggc tggttggcgt ctatagcaac cccgatcaat tggtgattta
     2041 tcccgatgga accaaagtcc acattgttgc tatgagcttt gaggcacagg tcatcactgg
     2101 cgaactcgtc ttaacaaacg aaacaaccag tgtcggttat tttacatttg aggaaattga
     2161 taccatggat ttctttggga gccataagca acgaatttat gatgcattgg aaaatcagaa
     2221 gactgccttc ttaaggtagg aatctcccgt agtgccggtg aaattgcatt atctgacttt
     2281 ataatattct aatgtttggt ggtattgaaa cggatcagtt attcttattt ttcatcctga
     2341 tcgggtccat tgtcctgctg ttcacggaat ggatccgtat tgatttaacg gcagtattaa
     2401 ttatcctgat gctcagcctt accggtgtgc ttgagccaga ggcggcattc tctgggttta
     2461 gcagtgagcc agccatttta ttggcgtctg tgtttgtgct gaacggggcg ctgtatcata
     2521 ccgggctatc tgaacaattg gggaatcgaa tcaaacaatg ggccggcaaa agctttgaac
     2581 agaccatcgg cgttgtgatg ccttccgtgg cgttgttatc cgcgtttact catcatgtga
     2641 ccattacagc actcatgctg ccgccaacgt taaagctggc gcgtgaaaat aacataccgg
     2701 cttccaaatt actgatcccc ttgtcatttg ccgcctcgct gggaacagcc atcaccatca
     2761 tcggcgcgcc tgcgttcctg atcgcagatg gcctgctgaa gcaagctcgt caacccggat
     2821 tgggaatctt ttccattgcg ccgatcgggc tggcattgac tctggctgga acaattttct
     2881 ttttattgtt gggacgtttc ctgcttccgg cacggcgcgg cgatgaggag gcaatcgatc
     2941 actttcgcct cgagggctat tccacagagt tggtgatcct gccggattct cccttcatcg
     3001 gtaaaaccat tcgtcaaatc gaagaggaac aaccgacgga attgaaggta tctgcctggt
     3061 atcgtgatga tcgaccgcgc agccggccct tcggcagcaa gaaggcacaa gccggagatg
     3121 tgctggttat tcggacgaat ccggacaggc tggcaaccat ccagcaggaa cctggtatcg
     3181 cactccgccc gttacagaaa tatgaagaag ccttttcgcc cacccaggat agtgataact
     3241 ccaaagaaga attagcctcg cggctggtgc aatgtgtggt tgctccacgc tccgatttaa
     3301 ttggcaagac gatcggtaaa gttgactttc ttcaaaatta tggcgtgatc gtcgtcagcg
     3361 tctggaggcg caaaggctgg ctacgaacgg agctatctcg tgtaaaactg caggaaggcg
     3421 atgtgctggt gatggtgggt gacaatacag cttttaaaca gatctccggt gatcggtcgt
     3481 tcttaatgtt ggttccattt aaaggggaac ccaagcctcg tcacaaagca cggctcgccg
     3541 gaattattat gctggcatcc attattttgg caaccattaa tgtgtttccg gttcaggtgc
     3601 cgttactggc gggtgccgtc atcgtgatcc ttagtggatg catttcgatg tcgcaggcgt
     3661 accaatccat cgatacgcgt atttatgtat tcatcgcagg tgctattccc ctggggctgg
     3721 ccatgcagga aaccggcact gccatattgc tggctaattg gatggaagga ctggtctcca
     3781 catgggacat ccattggatt ctcctcatgc tatttctcat taccgggctt gtgacgcagg
     3841 taatgtctga ctccgctact accgctttgt tcgcaccggt ggcaatcgcc ctggcccgca
     3901 gcctggatct tcccccggag ccgctcgtgg ttaccgtcgc aatggcctct gtgacctcct
     3961 tctttactcc gctgggtcat catgggaatc tactcatcta tggacctggc cgttatcagt
     4021 ttgcagattt tctgcgcgtg ggaattcctc tgacccttat cgtggcagtt attgtctcat
     4081 ttatggctcc catgatttgg cccatcatct aaatagctcg aagatcgcga ggcgttcccg
     4141 gggatcgtgg ggttttttat ttcagccaag gaggcttcca tcatgctcaa caaaatctcc
     4201 gaaaaatttc attcctgggc gaagggcagg tgggtactag ttgcactgat tgcatatgcc
     4261 ttgatggcag ggtacgtaat gcccgtggca gccgggatca ttgcatttgc cgccaacaac
     4321 agtgtgctgc cacttgactt gttgtttttc tataccccag agcaagccta tagcatgatc
     4381 gacaaatatg gggaagccgg tcgctccatt tattggaaaa tcgaactaac cgccgatatt
     4441 atttatccga ttacatacac attgtttttt gggctgcttc tatcctggtt attccaacgg
     4501 ggattcaaat cgaatcacca gatccaaaag tggaatgtac tgccagtcgg cggatggttc
     4561 tttgacatgc ttgagaatgt agggattgtt tccatgcttt ccgtataccc ggcaaaacca
     4621 gcatttcttg cctggctgac gatgatcttt ggttctctca aatgggcttt tgcagcggtt
     4681 agcattgtgc tcattctgat tggtctggta cgagcagtca tcaacggatt tagaaaacag
     4741 gaataaacct gcaggataca atacaaaatt ccctcaccag gagggatgtg ttaaatccat
     4801 caactttcta taattgaata cgaagctgat gatacgtact acatactgtt tgtacgattt
     4861 atgcgttttt aaaggttatc agaaaacctc ctatgcgtca gcccctcatc cgtgtcggtt
     4921 tactacttct gcttactgct tgtagcagct caacgggcac accaactttg gttaccccca
     4981 ctccggccgt cgcggttgtt gaaacaccca cgccgatgca ttttgaggag agactctttg
     5041 actatgattt gcaggaaccc gta
//
LOCUS       DASTJP010000008         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_631812, whole genome shotgun sequence.
ACCESSION   DASTJP010000008 DASTJP010000000
VERSION     DASTJP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631812"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..580)
                     /locus_tag="VFQ23_00155"
     CDS             complement(71..580)
                     /locus_tag="VFQ23_00155"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9905007.1"
                     /translation="MSAKLSWSSKKLVIVALSVAVLLASSIYPITNVGATPGSGVTGE
                     LIASGTLPEEVPSKFKTETGIVQADVSKITVIRHTIVPGGVFGWHQHGGPLWATVVSG
                     NLTIYHGDDPTCTGEVYPAGSVFMDSGNHTHNARNEGSENLVVLVTYMLPEGAAPRID
                     VPNPGNCAF"
     gene            complement(703..>2179)
                     /locus_tag="VFQ23_00160"
     CDS             complement(703..>2179)
                     /locus_tag="VFQ23_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9905008.1"
                     /translation="GRNYHQTGEYFRQAEELARKLNEPRLIAISLNNMGNWYFVTGQT
                     EQALQCHRQALEFFEGEHDEHGMAQSRAHMGMANLHHGDQLRAYEEYQHAIRIFRKLD
                     AKPELIPALIGACHTSYDETDYIPPRLPKQSLELALEARELARQVGWASGEAFAEWDI
                     SLGLAQCGLLGDALAHATSMFRIATEIEDPQRISGAYYALGFSYLLMHQAGLAIQNLE
                     AGLAVAKGFGSSWMIGNSTTELAIAYVLNDQLAKASALLDSVPLHETGHHTRAERRML
                     WAKGNLLLAENKPADALRIAEHLLESKQNSQNLQPIPALWKLKGEALLALKQFKEAEQ
                     ALDGAKQSAEEREALPLLWRIHAGLGWLHKDQKNLEESEREFASARLLIDRLGSNIPD
                     ESLRNEFIRVALQSLPMERVPSKRQRDAESFGGLTAREREVARLVAEGNSNREIAESL
                     FLSERTVENHVGNILMKLGFDSRAQVAVWAVEKGLRPTEKN"
BASE COUNT          472 a          616 c          548 g          543 t
ORIGIN      
        1 gattctccgt ttctttttga attgggcgag tgttcttgta gacaagggcc tcgccccttt
       61 tgaaattttt ttagaaggca caatttcctg ggttgggaac gtcgatgcgt ggtgcagcac
      121 cttcgggcag catgtaggtc acaagcacga ccagattttc actgccttca ttgcgggcgt
      181 tgtgggtgtg gttaccggaa tccatgaaga cgctgccagc cggatacact tcacctgtac
      241 aggtaggatc atcgccatga tagatcgtca ggttcccaga tacgactgtt gcccaaaggg
      301 gaccaccatg ctggtgccac ccaaacacac caccggggac gattgtgtgt cggatcactg
      361 taatcttgga cacatccgcc tggacaatcc ccgtctcggt cttgaatttc gacgggactt
      421 cttctggcag cgtccctgat gcaatgagtt caccagtgac tccggatccg ggcgtagcac
      481 ccacgtttgt gatcgggtat attgagctgg ccaacaacac cgccacgcta agcgcaacga
      541 tcaccagctt tttcgaactc cacgataatt ttgcagacat ttctacctcc atatgatttg
      601 taaagtatcg agaccgcgga acatctcgac cttgcagcat taaagcaaaa acacgatggc
      661 atgccatcgt ggaaaatact cagttaatca aaaatggagt acctagtttt tttctgtggg
      721 gcgcaatccc ttttccaccg cccaaacggc aacttgcgcc cgtgaatcga aacccagctt
      781 catcagaata ttgcccacgt gattttccac cgttcgttcg ctgaggaaca aggactcggc
      841 gatctcgcga ttggagttcc cttctgcgac caggcgagcc acctcccgct cgcgcgcggt
      901 gagtcctcca aatgattcgg cgtcacgctg acgcttcgaa gggactcgtt ccattgggag
      961 gctctgtaac gcgactcgaa taaactcatt ccgaagggat tcatctggaa tatttgaccc
     1021 cagcctgtca atgaggagac gcgcgctggc aaattcgcgt tcgctctctt ccaggttctt
     1081 ttgatctttg tgcagccatc ccaacccggc atggatgcgc cataataacg gaagtgcctc
     1141 acgttcttct gcactttgtt ttgccccgtc caacgcttgc tcggcctcct tgaactgttt
     1201 caacgccaac aaagcctctc ctttgagctt ccacaaggcc gggatgggct gcaggttctg
     1261 ggagttttgc tttgactcca gcaagtgttc ggcgatgcgc aacgcatcag cgggtttgtt
     1321 ctcggccagc aacaaattgc cttttgccca gagcatacgc cgttctgccc gtgtatgatg
     1381 accggtttcg tgcaggggaa ccgaatcgag aagtgcgctc gctttcgcca actgatcgtt
     1441 gagcacgtaa gcgattgcca actccgtcgt cgaattcccg atcatccagg aggacccaaa
     1501 tccctttgcc acagcgagac ccgcctccag attctggatg gcgaggcctg cctgatgcat
     1561 taacaagtag ctaaacccga gtgcataata cgcacccgag atccgttgtg gatcttcgat
     1621 ctctgtagcg attcgaaaca tggatgtggc atgtgcgagc gcatccccaa gcaatccgca
     1681 ttgcgcgaga ccgagtgaaa tgtcccattc agcaaacgcc tcgcctgacg cccagccaac
     1741 ctgacgggca agctcccgtg cttcgagcgc cagctccaga ctctgctttg gcaatcgagg
     1801 tgggatatag tctgtttcat catagctcgt atgacaggcc ccgatcaaag ccggaatcag
     1861 ctcaggtttc gcgtctaatt ttcgaaagat ccgaatggca tgctggtact cttcataggc
     1921 acggagctga tctccatgat gaaggtttgc cattcccatg tgtgcgcggc tctgtgccat
     1981 tccatgctcg tcgtgctcgc cttcgaaaaa ttcgagcgcc tgccgatggc attgcaaggc
     2041 ctgctccgtt tgcccggtga cgaaatacca gttgcccata ttgttgaggc tgatggcgat
     2101 caatctgggt tcattcagtt tccgtgccag ttcttcagcc tgacggaaat attcgccggt
     2161 ttgatgatag ttgcggccc
//
LOCUS       DASTJP010000009        15685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_631740, whole genome shotgun sequence.
ACCESSION   DASTJP010000009 DASTJP010000000
VERSION     DASTJP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15685
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631740"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..713
                     /locus_tag="VFQ23_00165"
     CDS             246..713
                     /locus_tag="VFQ23_00165"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HET9905009.1"
                     /translation="MRVYFAGPLFCEAERVFNLRLTEKLEAQGYEVFLPQRDGAELQK
                     PPYNNMPNDELQKTIFKRDRDKLIQADIFLFVLDGRVPDEGGCVELGIAYGQKHFLQQ
                     DKLLIGLHTDMRVAFPDGKLNAMISGALDFTMSNEEELIAKIEAFGSARNARV"
     gene            768..1949
                     /locus_tag="VFQ23_00170"
     CDS             768..1949
                     /locus_tag="VFQ23_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905010.1"
                     /translation="MNLANTKLLAGIQRDLAIVAISAFIWGLGEGLFIFFLPLALQRW
                     NADAVQIGVVLSMIGVAMAVVQVPAGYLSDRFGTRPLIFSSLLLGIASAVIMSTARSL
                     PFFVAGLLAYGCTSFISAPVNSYVTSLRGNWTIQRAVTFRAAAFQVGAILGPILGGWI
                     AATDGLSIVFRYSAGLFLAATLVMSLAKRPVELEPLEASIHLVSPLANRRFVGLLTVV
                     FFTIIAISVPQQLTSLYLQNVQHISIQQIGTMGTIASVGTAIIMFSLGNLRVSTGMLI
                     GQLLLAGFALLMWQGQSVMAYFAGYFFVGGYQLYRSMALAFSRSLVKVGDTGLAYGLI
                     ETGNALAVVFAPLAAGFLYNYRPEAVYIASLGALAITVGLNRLLFQEKRNEILPAHTS
                     Q"
     gene            2027..2581
                     /locus_tag="VFQ23_00175"
     CDS             2027..2581
                     /locus_tag="VFQ23_00175"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9905011.1"
                     /translation="MLQGKKTLLRSISRDDLVRLWQFNNDVEVELAGGGDPPIPQSLA
                     RLYAEYDQESSKGGRDGSTFAIEADGQCIGQCALFNFNETSQTCELGITIGDKAYWGR
                     GYGRDALQCLLDYAFRLRNMRRVYLSVNGNNQRAIRAYEVCGFVEEGRLRAHVWNDGR
                     FVDLVLMGLLKEEWNRLQAQVSSE"
     gene            2665..3114
                     /locus_tag="VFQ23_00180"
     CDS             2665..3114
                     /locus_tag="VFQ23_00180"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9905012.1"
                     /translation="MKTVTYTYRISVNAKPDVAFAYIADLTRHSEWNDHLRVTAKTPG
                     EIKPGSEYHSVGKTLYEDRHNDLWITAYQPPTLFSFVARDPDFKDIAHEFRINPQNDG
                     SLVERTVTVQMSAFMEFLWRLLFFPLINRRENYRSMAALQTQLNRMQ"
     gene            3219..3974
                     /gene="dapB"
                     /locus_tag="VFQ23_00185"
     CDS             3219..3974
                     /gene="dapB"
                     /locus_tag="VFQ23_00185"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET9905013.1"
                     /translation="MINVAVSGALGRLGSVIAKGVSNADDMHLSGVYSPSHAGRVVEG
                     VEVESSVENINAHVIVECTHPNVVMANLRQWHAKGVPVVVGTSGFTKERIEEVKSFWG
                     ENDPGCLIVANFSIGAVLMMRFAEIASAYFDSAEIIERHENTKPDAPSGTALATVMRM
                     SKHRKQADEIKNDEIIKGARGGLVSGVRVHSLRMKGLLSDQEVAFSNLGETFSIIHRS
                     TSYESFANGALVAIRYVQTIKGVAIGLDAALDF"
     gene            4204..4497
                     /locus_tag="VFQ23_00190"
     CDS             4204..4497
                     /locus_tag="VFQ23_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905014.1"
                     /translation="MTETRIDRELAEERKEQAAEIATRRLILWITALVISIAVLMFRI
                     GENLWPQWLVVYRLQIIALLALFTIGSLLLSPLLIEYSKDPRPLSGPGKRPGF"
     gene            4530..5153
                     /locus_tag="VFQ23_00195"
     CDS             4530..5153
                     /locus_tag="VFQ23_00195"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET9905015.1"
                     /translation="MNETPKSSTQASVAAPMTDEQILASNLKMPAVLNQSVLIVNYDP
                     EWPRLFEREETRIRDALSTHALSIEHVGSTSVPGLAAKPKIDILLVVASSADESSYVP
                     SLENAGYTLQIREPDWYEHRMFRGPDTDVNLHVFSQGCEEIEKMLLFRNWLRNNLDDR
                     QLYEQTKRDLAKQTWKYMQNYADAKSAVVAEIMGRAKKAVKPNEAES"
     gene            5150..5839
                     /locus_tag="VFQ23_00200"
     CDS             5150..5839
                     /locus_tag="VFQ23_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905016.1"
                     /translation="MKTTNKFWLATCLLIFLAACSGEPKFINHPAPNLSVSFDAFNND
                     ESLNALGCDEIQIPSDLLGGLDPSYPIAICAIRPDEVSEELRAEIDSGQFFYYTGGLF
                     GNYIRYVIYQNGEFLLIKTAEDFRRIFAPIESSQEALSYVLAVKNLSAYYGLKHDPAY
                     KYEVNTIEDTYITPDSDGFLVHLFHEQVFGCGPHWTSEVDVRVSVEGNVEEVASQPIL
                     RDPNLDDLCVD"
     gene            complement(5885..6961)
                     /locus_tag="VFQ23_00205"
     CDS             complement(5885..6961)
                     /locus_tag="VFQ23_00205"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET9905017.1"
                     /translation="MTLEMTTDIQSLLGDKAESLLGFDSPKISKDRLHLPGPDYIDRI
                     FMQSDRNNRVLGNLAWMYNTGRLGRTGYLSILPVDQGIEHSGGASFAKNPDYFDPENI
                     VKLAIEGGCNAVASTFGVLGVVARKYAHKIPFLVKINHNELLTHPNNFEQILFGTVEQ
                     AHDMGAAAIGATIYFGSDDSHRELIEIAEAFAYAHELGMATVLWCYLRNKAFKVDKDY
                     HVSADLTGQANHLGVTIQADIIKQKLAENNGGFTALNSGDSSYGKLDKRMYSELSSDH
                     PIDLCRYQVANCYMGRIGLINSGGASGENDFAEAAATAVINKRAGGMGLISGRKAFQR
                     PMAEGVKLLNTIQDVYLDQSITVA"
     gene            7220..7741
                     /locus_tag="VFQ23_00210"
     CDS             7220..7741
                     /locus_tag="VFQ23_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905018.1"
                     /translation="MDDFFQKVTSQQDPFKRLVSFIPGFGGYIERQNRRDADKLLRDT
                     VARRFEEQYQRASQLQTELVSAGKIEFVDDMEKAVIKLRTFIDKISTAPRGYSGLFDA
                     VKINEKELESIYQFDAAFFDLAEQVGRALDNVEASLGDEAALPAAIRNITSLARTAVE
                     TYNRRSEAVTGTK"
     gene            7793..9241
                     /locus_tag="VFQ23_00215"
     CDS             7793..9241
                     /locus_tag="VFQ23_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET9905019.1"
                     /translation="MWHEYINATTVDQVLQTLKDKGARARIVAGGTDLILELERGVRK
                     GIETLIDVTRIPDLDKITIDEEDVIHLGPLVTHNHCVQSPLIRERAYPLARAAWDVGA
                     PQIRNRGTVSGNLITASPANDTITPLMALNASVTLQSTNGTRTIALKDFYQGVRKIDM
                     QPDEMLVDISFPAMKDTARGTFIKVALRRAQAISIVDVAIILDLEGDTVKSASIALGS
                     VAPTIVRATEAEQFLEGTQLTEETLAEAARLTMSASKPIDDIRSSAAYRREMVRVCTL
                     RGLKAIRGGNEQAGMPADAVLLWGEHNVNGQVNAAHEAPSSPIETTINGKHYAVNTGH
                     EKTLLRFLREDVGLIGTKEGCAEGECGACTVFLDGSAVMACLVPAPRAHGAEIVTIEG
                     LASSFPVEEGQGVSARLHSVQKAFVEHGAVQCGYCTPGFIMSAAKLLEEKPNPTDHEI
                     EQAITGNLCRCTGYYKIVQAIRSAAEDASRTR"
     gene            9250..9672
                     /locus_tag="VFQ23_00220"
     CDS             9250..9672
                     /locus_tag="VFQ23_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905020.1"
                     /translation="MGKYTSVRKAAPPPRNREVHPLMRGIGCIMMIIVPIVAYGSAVL
                     LVNYGVSRGWPIPPSWLGTPTFHPLLWRLQGLTTLLGFLQTQTNLMANLVFAIAITVV
                     IGGIMAMIYGYIYKLFGPPQYGPTDAPPERVKVKKYTR"
     gene            9707..11902
                     /locus_tag="VFQ23_00225"
     CDS             9707..11902
                     /locus_tag="VFQ23_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET9905021.1"
                     /translation="MTEYKTIGKSVPRVDAHGKVTGETPYSGDLAMDGMLHMKMLFAG
                     RPHARIKAINVEKAEAAPGVVAIYTAKDIPVNEHGLQWQDQPTLCGVGSTKPGADIVR
                     FVGDQVAAVVAESEAEAAAAVKLIEVDYEDLPIVDDPLEAMKPDSPRIHEFIGDSNIC
                     VHYKIRKGNVEDAFANADVIVESEYRTPVQEHAYLQPEAGLAYIDEEGRITIACGGQW
                     THEDQNQVAHSLNLPIRGIRVVYPAVGGAFGGREDMSVQIVLALAAWKLQRPVKIVWS
                     RQESIIGHGKRHAVILRAKWGATKDGKVIAIENEIIGDGGAYMYTSNKVLGNSAITST
                     GAYNIPNVKTDVYGVYTNNVPGAAFRGFGAPQALFMAEMQMNKLAEKLGMDPVEFRLK
                     NALKDGDAMGVGTPSPSPVTVTECIEAARDKFEWRHSPQPTPPPEGEGTLVRGQGFAA
                     GFKNIGFSFGYQENCWAKIELQGKSEIEQVILHHASAEVGQGTHTVMMQMAAEAIGVP
                     FEKVKLISSDSATMGNSGSVSASRMTFMSGNAIRGAAEVALNKWNAEERPAVGEYKYI
                     APRTTPFDHETGYSTPNFAYAFVAQAIEVEVDTETGHLRVIRVISADDVGKPINPALV
                     EGQVEGAVVQAQGYAVLEDFKSKEGRVLSDQLSTYLIPTIWDIPEKVETVFLEIPDPN
                     GPWGARGVGELPFLTVAPGIAAAIHDATGVWIDEFPFTPERVLRALGKI"
     gene            11945..12832
                     /locus_tag="VFQ23_00230"
     CDS             11945..12832
                     /locus_tag="VFQ23_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9905022.1"
                     /translation="MSLLYRGEVKDLNNETRQEAGGSFIQLPDGITHYEMGGPETGKS
                     VILIHGFSVPYFIYDPTFEFLKQSGFAVLRYDLFGRGFSDRPHLQYKLDLFVKQLADL
                     LDALHITEPASLIGLSMGGPIAAAFATHYSARVNKLVLIDPVGAKPIRLSTALKVAKV
                     PVVGEIGFGLIGNEALARSVASDFFDPSLVEKLQSQYIIQMQYKGFKRAILSTIRNNM
                     LGSFINVYERLGKMDKEILLLWGRNDATVPFEHSEILCAVLKNARFLAVDDCGHIPHY
                     EKPAEINPILLEFLRDHVS"
     gene            12822..13544
                     /locus_tag="VFQ23_00235"
     CDS             12822..13544
                     /locus_tag="VFQ23_00235"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HET9905023.1"
                     /translation="MFHRFFDYFSHNKYRLTMFLLLAGATIFSVVIWRVRSEVSGTVH
                     YAFLIWNLFLAWIPFIIAYITYTAPLRRQQIYIVVPVAAFFWLIFFPNAPYILTDFQH
                     LAGVWRDVPVWYDVMLLLWFAFTGLLLGIVSLFLMQEVIRREFGHWVGWGFVAIVVAL
                     SSTGIYMGRFLRWNSWDILRNPAGIALFTFERIQDPSLQSIGFTGLFGAFFLFLYITF
                     YTFGHLLLERPVEDTSPQGKFT"
     gene            13541..14035
                     /locus_tag="VFQ23_00240"
     CDS             13541..14035
                     /locus_tag="VFQ23_00240"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9905024.1"
                     /translation="MNKNDIKILYQYNDWASARIFNAAKNVTQDQFLAPASFPHGGLR
                     GTLVHALFAAWIWRERWEGTSPMERLKPEDFPTVDSLHTRWLEEGKALTSFIEGLTDE
                     KLEATFHYKNTQGKPFERVLWHAMAHVINHGTQHLTEAAAILTDLGHSPGDIDMILFL
                     IEKY"
     gene            14116..14868
                     /locus_tag="VFQ23_00245"
     CDS             14116..14868
                     /locus_tag="VFQ23_00245"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HET9905025.1"
                     /translation="MFNRLRYSFIRNRYSIAVFALLNLACALCIALVAARIAYSDTRG
                     HLGLIWNLFLAWIPFMLAYFAHTISWRRIWLYLIIPVVALFWLIFFPNAPYMLTDLQD
                     LTRTTEEAPLWYDVIIVVWCTWTGMLLGVISLYLMQDIIHRAFSRTAGWIFVFVISAL
                     SSFGIYIGRFVRLNSWNILLDPRETAMEILGIVIDPSMRLAAFTILYTFFFLFVYLLL
                     YSFSHLLQEHHVRAQDSPEQPAGTQPHHAEQR"
     gene            14852..>15685
                     /gene="yqeB"
                     /locus_tag="VFQ23_00250"
     CDS             14852..>15685
                     /gene="yqeB"
                     /locus_tag="VFQ23_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HET9905026.1"
                     /translation="MQNKDKALVLLRGGGDLATGVALRLHRSGIKLVITELAQPLAVR
                     RTVSFAEAIYEGHQTVEGVTARRVQAEGLQGWNRADEIPIVVDPDANVLTLIDFLVVV
                     DARLMKSTPSALPVDVPLHIGLGPGFHADRDCHAVVETRRSHTLGRVYWTGSAQADSG
                     EPEGDRRRVLRAPANGILTGHAQIGDHLEEGQLIAEVKNETEVSKIESPFKGVLRGLI
                     HDGVEVRQGLKIGDVDPRDDPSACYLVSDKALAIGGGVLESLLSRKEIRERLWTPTAP
                     IG"
BASE COUNT         3852 a         3836 c         4060 g         3937 t
ORIGIN      
        1 tgcatccgct ttatcaatcg ttacaattat gctcgcagtg ttattcctga atgagaaaat
       61 ttcaaaactc caggcgctgg aatcgtcatg tcgatagtcg gtataatcgt aactgcattg
      121 tagaatagga ttcgaacaat caatgcaatt ttccagtcgg aggttggcga ttagcccgcc
      181 tttgcgcatt gttgttactc tattacacaa ccaggtcttt gtttgtcgac cacaggaagg
      241 atgatatgcg agtttacttt gcgggaccgc tattttgcga agcagaaaga gtattcaatc
      301 tgcgtctgac tgaaaagctg gaggcacagg gatatgaggt gttcctgcct caacgagatg
      361 gtgcagagct tcagaagccc ccctacaata atatgcccaa cgatgagttg caaaagacaa
      421 tatttaagcg agaccgagat aagctaattc aagctgatat ctttctcttt gtgctggatg
      481 gccgcgtgcc ggatgaaggt ggctgtgtcg agttaggtat agcgtatggg cagaaacatt
      541 ttttacagca agacaagcta ttgataggct tgcacacaga catgcgcgtg gcgtttcccg
      601 atggaaaact caatgcgatg attagtggcg cgctggactt taccatgagc aatgaggaag
      661 agttaatcgc aaagattgag gcgtttggga gtgcacgcaa tgccagagtg tagcgttgtt
      721 acgaggcaca agtaattgca cggtggctag cctgagtgcg gcgactcatg aatctggcta
      781 acacaaaact gctcgcgggc attcaacgtg acctggctat tgttgcgata tccgcgttca
      841 tttgggggct gggtgagggt ttgtttatct tcttcctgcc attggctctc caaaggtgga
      901 acgcggatgc agtgcaaatc ggagttgtac tgagcatgat cggggtagcg atggcggtgg
      961 tacaggtacc agccgggtat ctttctgacc gttttggcac gcgaccactt attttttcat
     1021 cgctactact agggattgcc tctgcagtga ttatgtctac ggctcgcagc ctgccttttt
     1081 ttgtcgccgg gttattggcc tatggttgca cttcgtttat tagcgcgccc gtaaacagct
     1141 atgtcacaag cttacgtgga aattggacca tacagcgcgc ggttacgttt cgcgctgcag
     1201 cattccaggt gggtgcgatc ctcggaccga tattgggcgg ttggatcgca gccacagacg
     1261 gactatccat cgtctttcgg tactccgccg ggcttttcct ggccgctacg ctggtcatgt
     1321 cgcttgcaaa acgtccagtg gagttggaac ctctggaagc atcaatccat ctcgttagcc
     1381 cactcgccaa ccgccggttc gttggattac tgaccgtcgt ttttttcacg atcatcgcga
     1441 tcagtgtgcc acaacaactt acctcccttt atctacaaaa cgttcagcac atttcgatcc
     1501 aacaaatcgg aaccatggga acgattgcaa gtgttggcac agccatcatt atgttctctc
     1561 ttgggaatct acgcgtatcc actgggatgc taattgggca actgctgctc gctggctttg
     1621 ctcttttgat gtggcagggg caaagcgtga tggcctattt cgcgggatac tttttcgttg
     1681 gtggatacca actgtaccgt tccatggcac tcgctttttc gcgctcgttg gtcaaggtcg
     1741 gggataccgg gttggcctat ggtctcattg aaacggggaa cgctctagca gtcgtctttg
     1801 ctccactcgc agcgggattc ctgtacaatt accgaccaga agcggtgtat atagccagcc
     1861 tgggcgctct cgccatcacg gtggggttaa acaggttgct ctttcaggaa aagcgcaatg
     1921 agattcttcc tgctcacaca tcgcagtaaa ttgcgatgca gttcgccagc cgccaataca
     1981 ctcccttggg cgccgcccca cagtttaagg aagaatgaga gaaatcatgc tgcaaggtaa
     2041 aaagacactt ctacgctcca tttcgagaga tgacctggtg cggctctggc aattcaacaa
     2101 cgatgtggag gtcgagttag ccggtggcgg agatccgcct attcctcagt ctttggctcg
     2161 gctatacgct gaatatgacc aggagtccag caaaggcggg agagacggtt ccacctttgc
     2221 cattgaagcc gatggccaat gtattggtca gtgtgcatta tttaacttca atgaaacctc
     2281 ccaaacgtgt gagcttggaa ttacgattgg ggataaggcg tactggggac gtggctatgg
     2341 acgcgatgct cttcagtgtc tgctggatta tgcattccgc ttacgcaata tgcgacgcgt
     2401 ttatctttct gtgaatggga ataaccaacg tgccattcgg gcttatgagg tatgtgggtt
     2461 tgttgaagag gggcgcttac gagcccatgt atggaacgac gggaggttcg tcgatttggt
     2521 gttgatgggt ttactcaagg aagaatggaa tagactccaa gcccaggtat ccagtgaata
     2581 attttcgata gtgatcatgt cagaactgca ctagataagc cgttgggtat ccaccgccac
     2641 tatcgacgac cggaggtaaa tttcgtgaag actgtgactt atacatatcg catttctgtc
     2701 aatgcaaaac ctgatgtcgc ctttgcgtat atcgccgacc tgactcgaca tagcgaatgg
     2761 aatgatcatc ttcgcgtcac tgcaaagaca cccggtgaga tcaaaccagg cagcgaatat
     2821 cattcggttg gcaaaacact gtatgaagac cgccacaatg atttgtggat cacggcctat
     2881 caaccgccaa cactattttc gtttgtcgcc cgtgaccctg acttcaagga tatcgcccat
     2941 gagtttcgga tcaatcccca gaacgacggc agcttagttg aacgtacagt cacggttcaa
     3001 atgtcagcct tcatggaatt tttgtggcgc ttactgttct ttccactgat taaccgacgc
     3061 gagaattatc gttcgatggc ggccctccag acgcaattga accgtatgca atagactgtc
     3121 ttgacatggt tgataagtcc gcttgtatgt gaagctacca ggcgctaaga ggaagaaatt
     3181 tatttagacc aacacattgt tttgcgagga gaaaaggtat gattaacgta gccgtttcgg
     3241 gcgcattggg tcgcctgggg agtgtaattg caaaaggtgt ttcaaatgcg gacgacatgc
     3301 acctaagtgg agtttatagt ccgtcacatg cgggacgagt tgttgaaggc gtggaagttg
     3361 aaagttccgt agagaacata aatgcacatg ttattgtaga gtgcacgcat ccaaacgttg
     3421 tcatggcaaa tcttcggcag tggcacgcca aaggcgttcc cgtggtggtt ggcacgtctg
     3481 gttttacaaa ggagcggatc gaggaagtga aatccttctg gggagaaaat gatcccggct
     3541 gtctcatcgt cgccaatttt tctattggtg cggtactgat gatgcgtttt gctgaaatcg
     3601 catctgctta tttcgattca gccgaaatta ttgagcgtca cgaaaacacg aaaccagatg
     3661 cgccttccgg cacggcatta gctaccgtca tgcgaatgag caaacatcga aagcaggcag
     3721 acgaaataaa gaacgatgag atcataaaag gggcgcgcgg cggtttggtg tccggcgttc
     3781 gcgtccattc cttacgaatg aagggtttgc tatcagatca ggaggttgca ttcagcaacc
     3841 tgggcgagac gttttccata attcatcgta gcacttccta tgaatccttt gccaatggtg
     3901 ctttggtcgc cattcggtat gtacaaacta tcaaaggtgt tgcaattggg ttagatgcgg
     3961 cgctggattt ttgagcaaat actgccatct gatacacctc tctgtgcgtt cactccaacc
     4021 gagccctgta ggacagaaga cggattttag gcaaattcta tataaacttt agactatttc
     4081 atgttcgacg caatgagcgt tacaagtata gattagttgt gtcgaaagtg gcttttgaaa
     4141 ctaaatatgc tattgctcgt agattcacta ttagttgaaa cagcaaagtt gttggaggta
     4201 aaaatgactg agacacgaat agaccgtgaa ctagccgaag aacgcaaaga acaggctgct
     4261 gaaattgcca cgcggaggct gatcctctgg atcacagccc tggtgatatc catagccgtt
     4321 ctgatgttca gaatcggtga aaatttatgg ccgcagtggc tcgtcgtcta ccgtctacag
     4381 atcattgcgc tcttagcgct gtttacaatt ggctctctcc tattatctcc actgctcatt
     4441 gaatattcca aagacccgcg tcctctttcg gggccgggta aaaggcctgg attctaatca
     4501 ggaagtttag gttgtttaga aaggtaagaa tgaatgagac gccaaaatca agcacgcaag
     4561 caagtgtagc tgcgccaatg accgatgaac aaatattggc ttccaatctt aagatgccag
     4621 ccgttctcaa tcaatcggtt ctcatcgtca attacgatcc tgagtggccg cggctatttg
     4681 aacgcgagga aacgcggatt cgagacgcac tcagtactca cgctctgtca attgaacatg
     4741 taggctcgac ctcagtaccg gggctggctg ccaagccaaa aatcgacatc ctgctggtgg
     4801 tcgctagctc agccgatgag tcgagctacg ttccgtcact tgaaaacgca gggtatacac
     4861 ttcaaatccg tgagccggac tggtatgaac accggatgtt cagggggcca gatacggatg
     4921 tgaatctcca tgtattttct cagggttgtg aagagattga gaaaatgctc ttgttccgaa
     4981 actggttacg caacaacctg gacgaccgtc agctctatga acaaacaaaa cgcgatctgg
     5041 cgaagcaaac ctggaaatac atgcaaaatt atgcagatgc caagagcgct gtagttgcag
     5101 agattatggg acgtgccaag aaagctgtga agcccaatga agctgaatca tgaaaactac
     5161 gaacaaattc tggctggcaa cctgcttgtt gattttcctg gcggcttgta gtggagagcc
     5221 gaagtttatc aatcaccccg cgccaaacct ttctgtttcc tttgatgctt tcaataatga
     5281 cgagtccctg aatgcgttgg gctgtgatga aatccagata ccttcagacc ttctcggcgg
     5341 attggaccca tcgtatccta tcgcgatttg cgctattcga ccggacgagg tatcagaaga
     5401 gttaagagct gaaatcgaca gcggacaatt cttttattac actggtggtc tgtttggaaa
     5461 ttacattcga tatgtcattt atcaaaacgg cgaattcctg ttaatcaaga cagcagagga
     5521 ttttcggagg atatttgcac caatcgaatc gtctcaggaa gccctaagtt atgttttggc
     5581 agtcaaaaat ctgtctgcgt attatggtct caaacatgac cccgcatata aatatgaagt
     5641 taacacgatt gaggatacct atatcacccc cgattcagat ggcttcctcg tccatttatt
     5701 ccacgagcaa gtattcggat gtggcccaca ctggacatct gaagtagacg tgcgtgtttc
     5761 cgttgaagga aatgtggaag aagtagccag ccagcctatt ttgagagacc caaacctgga
     5821 tgatttgtgc gtagattaat tttgaacggg cgacccaatg gaatggatcg cccgttcata
     5881 tcatttacgc taccgtaata ctctgatcca aatacacatc ctggatcgta ttcaagagtt
     5941 ttacaccctc tgccattggg cgctggaagg ctttgcgccc agagataaga cccataccac
     6001 cggcgcgttt gttgatcacg gcagtcgcgg cagcttcggc gaagtcattc tcaccggatg
     6061 cgccaccgct gttgatgagt ccgatgcgcc ccatgtagca gttagcaacc tgatagcggc
     6121 acaagtcaat agggtgatcg ctggaaagct cactatacat gcgtttatcc agttttccat
     6181 acgaactgtc cccggagttc agcgccgtaa aaccaccatt gttttccgcg agtttttgtt
     6241 tgatgatatc ggcttgaatt gtcacaccca gatgatttgc ctgaccggtc agatcagcag
     6301 agacatgata gtccttgtca actttgaatg ccttgttacg caaatagcac cacaagactg
     6361 tcgccatacc cagctcatgg gcataggcaa aggcttccgc gatttcgatc aactcacgat
     6421 gggaatcatc ggagccaaag taaatcgtgg cgccgatggc cgccgcgccc atatcgtgtg
     6481 cctgctctac ggtgccgaac aagatttgtt caaaattgtt cgggtgagtt aacagttcgt
     6541 tatggttgat ctttaccagg aatggaatct tatgagcata cttgcgagcg accaccccta
     6601 atacgccaaa ggtcgatgcc accgcattac acccgccttc gatcgccagc ttgacgatgt
     6661 tttcggggtc aaaataatcc gggttttttg caaagctggc gccacctgaa tgctcaatgc
     6721 cctggtccac tggcaaaatg gacagataac cggttctgcc aagccgtccg gtgttgtaca
     6781 tccatgccag gttaccgagc acgcggttgt tccggtcaga ttgcatgaag atgcggtcga
     6841 tgtaatcggg acccggtaaa tgcaaacggt ccttagagat ttttggtgag tcaaaaccaa
     6901 gcaatgattc tgccttatca cctagtaaag attgaatatc agttgtcatt tcgagcgtca
     6961 tgttgttcct ccttacctgg cacaatgcaa tgcagcaggc gggttgagtt tatatactcc
     7021 cagcgctgtc agaggcagca cttgagcaga gtagaaaatc cttttcttga ttataaccag
     7081 aaactcaggc tataatatac ttaaagttcc tgcagtagcg cccctccagg gacggtttgc
     7141 aatcgagctg ctgcacgaac aagtattgaa aggtgcgact cattcccagg aaacacgtat
     7201 atccttgcag gagacacaca tggacgattt ctttcagaaa gtaaccagcc agcaagaccc
     7261 atttaagcga ttagtatcat ttatcccggg ctttggcgga tatatcgaac gacagaaccg
     7321 ccgcgatgca gataagctcc tgcgagatac ggtggcgcga cgatttgaag agcaatatca
     7381 acgcgcttcc cagttgcaaa ccgaactggt cagcgcaggc aagatcgagt tcgtggatga
     7441 tatggagaaa gccgtcatca agctgcgtac gtttatcgac aagatcagca cagccccgcg
     7501 tggctattca ggtttattcg acgcagtcaa gatcaatgaa aaggaacttg aatccattta
     7561 tcagttcgat gccgcgttct tcgacctggc tgaacaggtg gggcgtgcat tggacaatgt
     7621 ggaagccagt ctcggtgatg aggctgccct tcccgctgcg atccgaaata ttacgtcgct
     7681 ggcacgaaca gccgtagaaa cgtacaacag gcgatctgaa gcagtcaccg gaacgaaata
     7741 aatttagatg gtagggaagg gcagctgccc ttccctacca ttcccgcaaa ttatgtggca
     7801 cgaatatatc aacgcgacca cggtcgacca ggtccttcaa acgctgaagg acaaaggtgc
     7861 gcgtgcgcgc atcgtggctg gcggcaccga cctcatcctt gaactggagc gcggcgttcg
     7921 taaggggatt gaaaccttaa tagacgttac tcgaattccc gatctcgata aaatcacaat
     7981 cgatgaagaa gatgtcattc atcttggtcc tctggtcact cataatcact gtgtgcagtc
     8041 accgttgatt cgtgagcggg cgtatccgct tgcgcgggcg gcgtgggatg tgggtgcacc
     8101 acaaatccgc aaccgaggca cggtttcagg gaacctgatc actgcttccc ctgccaacga
     8161 tacgatcacg cctttaatgg cgttgaatgc ctctgtgact ctgcaatcta caaatggcac
     8221 ccgcacaatt gcgctgaaag atttctatca gggtgtgcgg aagatcgaca tgcaaccaga
     8281 tgaaatgctg gtagatatct cctttccggc catgaaggac acggcccgcg ggacattcat
     8341 caaggtggca cttcgtcgcg cgcaagcgat ttccattgtg gacgtggcaa tcattttgga
     8401 ccttgagggg gataccgtca aatcggcttc cattgccctc ggttccgtcg cccccacgat
     8461 cgtccgggcg accgaggcag agcaatttct cgaaggcaca cagcttacag aagaaaccct
     8521 ggcggaggct gcacgcctga cgatgagcgc cagcaaaccc attgacgata tccggagtag
     8581 tgcagcttat cgccgtgaaa tggtacgcgt gtgcacattg cgcgggttga aagcaattcg
     8641 cggcggcaac gagcaggctg ggatgccagc tgatgctgtt ttattatggg gagaacataa
     8701 tgtaaatggt caggtcaatg cggcgcacga ggcaccttca tcccctatcg aaaccacaat
     8761 taatggaaaa cactatgccg tcaacactgg gcatgaaaaa actttacttc gatttcttcg
     8821 agaagatgtc ggtttgatcg gcacaaagga aggctgcgcg gagggcgagt gcggtgcatg
     8881 tactgtgttt ctggatggca gcgccgtcat ggcgtgtctt gtccctgcgc cgcgcgctca
     8941 tggagcagaa attgtgacaa ttgaaggtct cgcttcctcg ttcccggtgg aagaggggca
     9001 aggggtgagc gcgaggcttc actccgtcca gaaagcgttt gtcgaacacg gcgccgttca
     9061 atgtggttat tgcacaccgg gttttattat gtccgccgcc aagctgctgg aagaaaagcc
     9121 aaaccccaca gatcatgaga tcgaacaggc aattaccggg aatctatgtc gttgtaccgg
     9181 ctactacaaa atcgtacagg ccattcgcag cgcggctgag gatgccagca gaacgagatg
     9241 aggtgaaaaa tgggtaagta tacgtcagtg cgcaaagcgg cacctcctcc ccgcaaccgg
     9301 gaggtgcacc cactcatgcg aggtattggt tgcattatga tgattattgt gccaatcgtg
     9361 gcttatggat cagccgtgtt actcgtaaat tacggtgtat caagaggctg gccgattcca
     9421 ccaagctggc ttggaacacc gacgtttcat ccgcttctgt ggaggctgca gggattgacc
     9481 acacttctgg gatttcttca gacacaaacg aatttgatgg ccaatctcgt gtttgcgatt
     9541 gccatcacgg tcgtaatcgg tggcattatg gctatgatct atggctacat ttataagctc
     9601 tttggtccac cgcaatatgg ccccacggat gcaccacccg agagagtaaa agttaaaaag
     9661 tacacccgat aattctccaa cattcttttg atcccttaaa cattttatga ccgaatacaa
     9721 aacgattggt aaatccgttc cacgcgtaga cgcgcacggc aaagtaactg gcgaaacacc
     9781 ctactccggt gacctggcga tggatggcat gcttcatatg aaaatgctgt tcgccgggcg
     9841 accccatgcg cgcatcaaag ccatcaatgt agaaaaagcc gaagctgcgc caggtgtggt
     9901 agcaatctat accgcgaaag atataccggt caatgagcat gggttgcagt ggcaggatca
     9961 gcccacgctg tgcggggttg gttcaaccaa acccggcgcg gacattgtgc gatttgtcgg
    10021 tgaccaggtt gctgctgtcg tggcggagag tgaggctgaa gccgcggccg cagtcaaatt
    10081 gatcgaggtc gactatgagg atttgcccat cgtcgacgac ccgctcgaag ccatgaagcc
    10141 cgactcgccg cgcatccatg aatttatcgg cgattcgaat atctgtgtgc attacaaaat
    10201 acgcaaagga aatgtggagg acgcatttgc caatgctgat gtaattgttg aaagtgaata
    10261 tcgtacgccg gtgcaggaac atgcctatct tcaaccggaa gccgggctgg cctacattga
    10321 tgaagaaggg cggatcacga ttgcatgcgg cggacaatgg acgcatgaag atcagaacca
    10381 ggttgcccat tccctaaatt tgccaataag agggattcgc gtggtgtatc cggcagtcgg
    10441 cggggcgttc ggcggccggg aagacatgtc tgtacagatt gtgctggctc tggctgcctg
    10501 gaagcttcaa cgccctgtca agatcgtctg gtcgcggcag gagtcgatca tcgggcatgg
    10561 caaacgccac gcggtcattt tgcgtgccaa atggggcgcg acaaaagatg gaaaagtgat
    10621 tgccatcgaa aacgagatca tcggtgacgg cggcgcgtat atgtacacct cgaacaaagt
    10681 actggggaat tcggcgatca cttctactgg cgcgtataac attccaaatg taaagactga
    10741 cgtctatggt gtttacacca ataacgttcc tggcgcggcg tttcgcgggt ttggcgcacc
    10801 ccaggctttg ttcatggctg aaatgcaaat gaataagctg gctgaaaaac tgggaatgga
    10861 ccccgttgaa tttcgtttga agaatgcctt gaaagatggg gatgccatgg gtgtaggaac
    10921 gccttcgcca agccccgtca ccgtgacgga atgtatcgag gcagcgaggg ataaatttga
    10981 atggaggcat agccctcagc ctaccccgcc ccctgaggga gaggggacgc tggttcgcgg
    11041 tcaaggcttt gcagccggct tcaagaacat tggcttttca tttgggtatc aggaaaattg
    11101 ttgggcaaag atcgagttac agggcaaaag tgagatcgag caggtcattc tccatcatgc
    11161 cagcgcagaa gtgggacaag gcacacatac cgtcatgatg caaatggcag ccgaggccat
    11221 cggcgtgcca tttgagaaag tgaaactgat atcgtctgat tctgcaacaa tgggcaattc
    11281 cggttctgtt tcagcctcgc gcatgacgtt catgtctggt aatgccatac gcggcgcagc
    11341 ggaggtggcg ttaaacaaat ggaatgcaga agagcgcccc gcagttggcg agtataaata
    11401 tatcgcaccg cgcactacgc ccttcgacca cgaaaccggc tactccactc caaattttgc
    11461 gtatgccttc gtcgcgcaag cgatcgaagt ggaagtggat acggaaacgg gtcatctgcg
    11521 cgtgatacgt gtcatctctg cggatgacgt gggcaagccc attaaccctg cgctggtgga
    11581 gggtcaggtg gagggtgctg tcgtccaggc acagggatat gccgtgttgg aggatttcaa
    11641 atcgaaggaa ggtcgtgtcc ttagcgatca actgagcacc tatctgatcc cgacgatttg
    11701 ggatatccct gaaaaagtgg aaacggtctt tctggaaatt cccgacccga atggtccctg
    11761 gggcgcccga ggtgtcggtg agttgccttt tcttaccgtt gcacccggca ttgcggcagc
    11821 cattcacgat gcgaccggcg tgtggatcga cgaatttcca tttacgccgg aacgggtgct
    11881 acgcgcgttg ggaaaaatat aattacccgt aggcaagcgc agcagccctc ccctacgaac
    11941 caaaatgtct ttgctctatc gcggcgaagt aaaagattta aacaatgaaa cccgccaaga
    12001 agccggcgga tccttcattc agttgccaga tggaattaca cactatgaaa tgggtggccc
    12061 agaaacaggt aaatccgtca ttctgattca cggtttctca gttccatact tcatctacga
    12121 ccccactttc gagttcctaa aacaatcagg ctttgctgta ttgcgctatg acttgttcgg
    12181 acgtgggttt tccgatcgtc cacatttgca atataagctc gacctctttg tcaaacagct
    12241 cgcagatcta ctggacgcac ttcacatcac cgaacctgca agcctgatcg gcctttccat
    12301 gggtggaccg attgccgccg catttgctac tcactattcg gctcgtgtca ataaacttgt
    12361 tttgattgac ccggttggcg caaagccgat tcggctctcc actgcgctca aagtcgcgaa
    12421 agtgcctgtt gttggcgaaa tcggttttgg actgattggc aacgaagcac ttgcaagatc
    12481 ggtcgcttcc gactttttcg atccttcact cgtcgaaaaa ctgcaatccc agtacataat
    12541 tcagatgcag tacaaaggat ttaagcgcgc aatcctttcg accatccgca acaacatgct
    12601 gggctcattc atcaacgtct atgagagact tggtaaaatg gacaaggaga ttctattgct
    12661 ttggggccgc aatgatgcca ctgtgccttt tgagcacagc gaaatactat gcgcagtatt
    12721 gaaaaacgcc agatttcttg ctgtggatga ttgcggacac atccctcact acgaaaagcc
    12781 tgcagaaatc aaccccatcc ttttggaatt tctgagagat catgtttcat agatttttcg
    12841 attatttctc acacaacaaa tatcggctga cgatgtttct tctgcttgcc ggagcgacca
    12901 tattttctgt ggtgatctgg cgagtacgct ccgaggtaag cgggacagtc cattacgcgt
    12961 tcttaatttg gaatcttttc ctggcatgga taccattcat catcgcctac attacctata
    13021 cagccccgct gcggaggcag caaatttata tcgttgttcc ggtcgcggca ttcttctggc
    13081 tgatcttctt tccaaatgcg ccttatattc ttacagattt tcaacatctg gcaggggtat
    13141 ggcgggatgt acccgtctgg tacgatgtga tgctgctgct ttggttcgcg ttcactggac
    13201 ttttgctggg aatcgtgtcc ttatttctaa tgcaggaagt tattcgtcgc gagtttggtc
    13261 attgggtcgg ctgggggttc gtcgcaatcg tagtagcgct gagcagcaca ggcatttaca
    13321 tggggcgttt tctgcgctgg aactcctggg atattcttcg caaccccgcc ggcattgccc
    13381 tctttacctt cgaacgaatt caagacccaa gcttgcaatc cattgggttt acagggcttt
    13441 ttggtgcctt ctttttgttt ctttacatca ccttctatac gttcggtcac ctattgctgg
    13501 aacggccggt ggaagacaca tcgccacaag gaaaatttac atgaacaaaa acgacattaa
    13561 gatcctatat caatacaatg attgggcaag cgcaaggatt ttcaatgccg cgaaaaatgt
    13621 cacgcaagat caatttctgg ctccagcttc cttcccgcat ggcggcttgc gcgggacgct
    13681 tgtccacgcg ttgtttgcag cgtggatctg gcgcgaacgc tgggaaggga catcgcccat
    13741 ggagcggctg aagccggagg acttcccaac ggtcgattca ttacacacac gctggctcga
    13801 agaaggaaaa gctctgactt ccttcattga gggtctcaca gatgaaaagc tcgaggccac
    13861 ctttcactac aaaaatacgc agggtaaacc gtttgagcgc gtcctgtggc atgcaatggc
    13921 acatgtcatc aaccatggga cacaacatct cactgaagcc gccgccatcc ttacagatct
    13981 gggtcattct cccggcgata tcgatatgat ccttttcctg attgaaaaat attagaaatc
    14041 attcgcaaat acactaatct acagagtgcc taatttctct cagcgtctct gaggctctgc
    14101 ggcaaatatc catttatgtt caacagactc cgctattctt tcattcgtaa tcgctacagc
    14161 atcgcagtct ttgccttgct taacctggcc tgtgcacttt gtattgccct ggtcgcggca
    14221 cgcatcgcat acagtgacac acgcggacac cttgggctga tctggaatct tttcctcgcc
    14281 tggattccct ttatgctggc gtactttgcg catacgattt catggcgacg catctggctc
    14341 tatcttatta ttccggtggt tgcattgttt tggctgattt tcttcccgaa tgcgccttat
    14401 atgctcactg acttacaaga cctgactcga accaccgagg aggctccctt atggtatgac
    14461 gtgatcatcg ttgtttggtg cacgtggaca ggcatgttac tgggtgtgat aagcctctat
    14521 ctgatgcagg atattattca tcgtgcgttc agtcgcacag caggctggat ctttgtattc
    14581 gtgatttcag ctctgagcag ttttggcatt tacatcggca ggtttgtccg cctcaactcc
    14641 tggaatattt tgctcgaccc cagagaaaca gcgatggaaa tcctcggcat tgttattgac
    14701 ccgagcatgc gtctcgcggc gttcaccata ctctatacat tcttctttct gtttgtctat
    14761 ctcttgcttt attcatttag ccatttgctt caggaacacc acgtccgcgc acaggattca
    14821 cctgaacaac ccgccggaac acagccacac catgcagaac aaagataagg ctcttgtgtt
    14881 attgcgcggc ggcggcgacc tggcgaccgg tgttgccttg cgattgcatc ggtctggaat
    14941 taagctcgtc atcaccgaac tggcacagcc cctggcagtg cgccgcacgg tctcatttgc
    15001 ggaggctatt tatgagggtc accagaccgt cgaaggcgtc accgcgcggc gcgtccaggc
    15061 ggaaggactt caaggctgga atcgtgcgga tgagattccg atagttgtcg acccggacgc
    15121 aaacgttttg acattgattg atttcctggt tgtggtcgat gcccggttaa tgaaatcaac
    15181 tccctccgcc ctgccggtgg acgttccact tcacatcgga ctgggccccg gattccacgc
    15241 cgacagagat tgccacgctg tagtcgaaac ccgccgcagt cacacccttg ggcgcgtcta
    15301 ctggactgga tcagcccaag cggactcggg tgaacccgaa ggcgaccgga ggcgcgtcct
    15361 gcgggcgccg gcaaacggaa tattgaccgg tcatgcacag atcggagatc atctggaaga
    15421 aggccaactc attgccgagg ttaagaacga aactgaggtt tccaaaatcg agagcccatt
    15481 caaaggcgtc ctgagaggct taattcacga tggagtagaa gttcgccagg ggctcaagat
    15541 tggcgatgtt gacccgcgtg atgacccaag cgcctgctat cttgtatcag ataaggcatt
    15601 agccattggt ggcggggtat tggagtcctt actttcaagg aaagaaataa gagaaagact
    15661 atggaccccc actgccccca tcggg
//
LOCUS       DASTJP010000010         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1054355, whole genome shotgun sequence.
ACCESSION   DASTJP010000010 DASTJP010000000
VERSION     DASTJP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054355"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VFQ23_00255"
     CDS             <1..805
                     /locus_tag="VFQ23_00255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9905027.1"
                     /translation="GFKFSPNPYFAQDLPHYYTNMGLTAENVAVKYGISREDQDEFSL
                     KSHQKAAQAVNSGLFDPELVPLDVEVLEMNGSEKPVKRMFTVSRDEGPREDTNLNALS
                     RLKPAFKEGGVVTAGNSSQMSDGAAAVMVMSAESAKQLGLTPLARFVSFAVGGVPPEF
                     MGIGPIVAIPKALKLAGLTLNDIDLVELNEAFAAQSLAVIRTLEMDESKVNVNGGAIA
                     LGHPLGCTGSKLTTQLIYEMGRRKSKYGMVTMCIGGGMGAAAIFENLQN"
     gene            858..1187
                     /locus_tag="VFQ23_00260"
     CDS             858..1187
                     /locus_tag="VFQ23_00260"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET9905028.1"
                     /translation="MPLTVSQLMEKMPSAFIPEKGVGVDAIIHFKFTGAEAGEWNARI
                     RDGVVNVANGAPVNPANMTLTADSEDYVKIFTGELDGMQAFMQGKIKLGGDLNLAMKL
                     MQMFKIR"
     gene            complement(1225..1989)
                     /locus_tag="VFQ23_00265"
     CDS             complement(1225..1989)
                     /locus_tag="VFQ23_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET9905029.1"
                     /translation="MDVPTYLNRLHFSRPIRPDAETLRELQIAHMRNIPFENLDIGLK
                     RKIQLNEDALWEKIIVQGRGGFCYEINGLFAWLLKQIGFEVTYLNARVFNKRDENLGI
                     EFDHLSLLVQIQNQSARWLVDAGFGDSFNEPLSFEERGEQVQGLRAYRLEQTPAGYIT
                     WQKNYDGSWEKQYFFDLTPRDFPAEYEAGCQYHQTSPRSSFTRSSIISRATPNGRVSL
                     EDKWLILTEHGKREKRPIADQDEYNTLLKKYFDVQL"
     gene            2131..3426
                     /locus_tag="VFQ23_00270"
     CDS             2131..3426
                     /locus_tag="VFQ23_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9905030.1"
                     /translation="MIKPATDVQSIERQFQDVHLLLTNVIDRLSRTGNPLVSAAIQDM
                     THVSKTVQKLEQGVAEHLNVRQSQLGALMGVGHANNSSLGLERVLEQVMDTLIALMRA
                     ERGFLMLREPNGELSVRMARGITQFKVDEEILKVSNSIMRKVAASGESILTTNAQQDP
                     RFEKQVSVAVYQLRSILCAPLKLKNDLIGVIYVDNRAHAGIFSESDLGLINAFADQAA
                     VAIHNAQLFEDLKRTNFQLEAANMELQIAYEATLDGWVRALDLRDKETEGHTQRVTVL
                     TERLARAMGMDGDELVHVTRGALLHDIGKMAIPDGILLKPGQLTDDERKLIQKHPIYA
                     YEMLSPIEFLRPALDIPYCHHEKWDGSGYPRGLRGEEIPFSARIFPVIDVWDALTSDR
                     PYRKGMPQNVVFHHIQADSGKHFDPRVVNAFMEMSDLSV"
     gene            complement(3478..>3891)
                     /locus_tag="VFQ23_00275"
     CDS             complement(3478..>3891)
                     /locus_tag="VFQ23_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905031.1"
                     /translation="ISALLAVRISKKFGLINTMVFTHIPSSILLMMIQWMPTLWPAVV
                     LLLLRFSISQMDVPTRQSYTMAMVEPDERSAASGVTAIARSLGSAISPALTGLFFSVP
                     ALFNLPFFLCGGLKIIYDLLLFREFRTVKPPEENI"
BASE COUNT          994 a          891 c         1063 g          943 t
ORIGIN      
        1 agggttcaag ttttcaccga atccatactt tgcccaggac ctgcctcact actacaccaa
       61 catggggctt acggctgaga acgtcgccgt caaatatggg attagccgcg aagatcagga
      121 tgaattctca ttgaagagtc accagaaagc cgcccaggcc gtcaactccg gactcttcga
      181 ccctgaactt gtgccgctgg atgttgaagt gttggaaatg aacggaagtg aaaaacctgt
      241 caagaggatg ttcacagtat cgcgcgatga gggtccgcgc gaagatacca atctgaatgc
      301 attgtccagg ctcaagcccg ctttcaaaga aggcggagtt gtcaccgcag gcaattcttc
      361 gcagatgtca gatggcgcgg cggcagtgat ggtgatgtct gctgagagcg cgaagcagct
      421 tggtctgact ccacttgcgc gatttgtttc atttgcggtt ggcggagtgc ctccggaatt
      481 catgggaatc ggtccgattg tggcaatccc caaagcgctg aaacttgccg ggctgaccct
      541 gaatgatatc gatttagtcg agctcaatga agccttcgct gctcagtctt tagcagttat
      601 ccgcacgctc gaaatggatg agagtaaagt caacgtcaac ggtggcgcaa tcgctttggg
      661 tcatccgctt ggatgtaccg gttcaaaact gaccacgcaa ttgatctacg aaatggggcg
      721 tcgcaaatcg aagtacggaa tggtgacgat gtgtatcggc ggcggtatgg gtgcggcagc
      781 gattttcgag aatttacaga attaactata ggggcacgac atgtcgtgcc cctgccgaaa
      841 acaaaaggag aatttatatg cccctcacag tatcacaact catggagaaa atgccgagtg
      901 cgttcatccc tgaaaaagga gttggcgtgg atgcaatcat tcacttcaag tttaccggcg
      961 ctgaagccgg cgaatggaat gccaggatcc gagatggagt ggtcaatgtc gcgaatggcg
     1021 cgccggtcaa tccggccaat atgaccctga cggctgactc agaggattac gtgaaaatct
     1081 ttactggtga attggatgga atgcaggcct tcatgcaggg caagattaaa ctgggtggtg
     1141 acctgaacct ggcgatgaag ttaatgcaga tgttcaagat caggtaatta caaaccacgg
     1201 aagtctcgta gacttccgtg gttttcataa ttgaacatcg aaatatttct ttaataacgt
     1261 attgtattca tcctgatcgg cgattggtcg cttttcgcgt ttgccatgtt ccgtaaggat
     1321 gagccacttg tcctccagag aaacgcgtcc gttgggcgtc gctcggctga taatactgct
     1381 ccgggtgaag ctggagcgcg gggaggtttg atggtactga catccagctt catactcagc
     1441 ggggaagtcg cggggcgtga ggtcgaagaa atattgtttt tcccatgaac catcatagtt
     1501 cttctgccac gtaatgtagc ctgcaggtgt ttgctcaaga cgatacgcgc gcagaccttg
     1561 cacctgctca ccgcgctctt cgaaactcag gggttcattg aacgagtcgc caaagcctgc
     1621 atccacaagc cagcgcgcag actgattctg aatttgtacc agcaaggaaa gatgatcgaa
     1681 ctctattcca agattttcat cacgcttatt aaatacgcgc gcgtttagat aggtgacttc
     1741 aaaaccgatt tgttttagca gccaggcaaa cagaccgtta atttcataac aaaacccgcc
     1801 gcgaccctgg acgatgatct tctcccacag cgcgtcctca ttgagttgga tctttcgctt
     1861 gagtccgata tcgagattct cgaaaggaat gttcctcata tgagcgattt gtaactctcg
     1921 cagcgtttcg gcgtctggtc ttataggtct ggagaagtgc aggcggttga ggtaggtggg
     1981 aacgtccatt tgaggaatta tagcgtattg cggataggca ataaggtttg agtctcttca
     2041 caggggtgaa ttcattaatg gattcagata ttttgagaga tttatgcaac atccagccct
     2101 gaaaatttac tgtgtatatt ggagtttggc atgatcaaac cagcaactga tgtccaatcc
     2161 atcgaacgtc aatttcaaga cgtgcatctc ctcttgacga atgtgatcga tagactttcg
     2221 aggacgggga acccacttgt ctcggcggct attcaggata tgacgcacgt ttcgaagaca
     2281 gttcaaaagc ttgagcaggg tgttgcagaa catttgaacg ttcggcagag tcagcttggc
     2341 gcgttgatgg gggtggggca cgctaacaac tcgtccctgg gcctggagcg agtcctcgaa
     2401 caggtgatgg atacgctaat cgccctgatg cgcgccgaac gcggtttcct gatgcttcgc
     2461 gaaccaaacg gggaattgtc cgttcgtatg gcgcggggca ttacacagtt caaggtcgat
     2521 gaagaaattc tcaaagtcag taactcaatc atgaggaaag tagctgcgtc cggagaatcc
     2581 atccttacca ccaatgctca gcaggacccg cgctttgaaa aacaggtgag cgtggctgtg
     2641 taccaactgc gatccatctt gtgcgctccg ttgaaactga agaacgattt aattggggtt
     2701 atttacgtcg ataatcgcgc acatgccggc atattttcag agagcgacct aggattgatc
     2761 aatgcctttg ccgatcaggc tgccgtcgcc atccataatg cacagttatt tgaagatttg
     2821 aagcgaacga attttcagct ggaagccgcc aatatggaat tacagatcgc ctatgaagcc
     2881 acgcttgatg gctgggtgcg cgcgctggat ctgcgcgaca aggaaacaga gggacatacc
     2941 cagcgtgtga cagtcctaac ggaacgacta gcgcgtgcga tgggaatgga cggtgatgaa
     3001 cttgtgcatg tgacgcgcgg tgcgctcttg catgacattg gcaagatggc gattcccgat
     3061 gggattttgc tcaagcccgg tcagttgacc gatgacgaac ggaagctgat tcagaagcac
     3121 ccgatctatg cttatgaaat gttgagcccg atcgagtttc tgcggcccgc attggatata
     3181 ccgtattgcc atcatgaaaa atgggatggg tcgggttatc cgcgcgggtt acggggcgaa
     3241 gaaatcccat tttccgcgcg tatctttccg gtaatcgatg tgtgggacgc gttgacttca
     3301 gatcggcctt atcgcaaagg aatgcctcaa aatgtggtgt tccaccatat tcaagcagac
     3361 tcaggcaagc atttcgatcc gcgggttgtg aatgcgttta tggaaatgag cgatttgtca
     3421 gtttgattaa tctcacgtag ggaacggcaa ctcgccgtgg tgcctttccc tacagtttta
     3481 tatattttct tccgggggct ttacagtacg aaattcacga aataacagca ggtcataaat
     3541 aattttcaat ccaccacaga gaaaaaaggg gaggttgaac aacgcgggaa cgctgaaaaa
     3601 caatccggtc aacgcgggtg aaatggccga gccgagggag cgcgcgatgg cggtcacgcc
     3661 ggaagctgcg gaacgttcgt cgggttccac cattgccatc gtataagact gccgtgtggg
     3721 aacatccatt tgcgagatgc taaagcgtaa caggagtaag acaactgcag gccagagggt
     3781 aggcatccat tgaatcatca tcagcaaaat gctggaaggg atatgcgtaa agaccatggt
     3841 attgatcaaa ccgaattttt tggagatgcg cacagccagt aaggctgaga t
//
LOCUS       DASTJP010000011        22744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_735730, whole genome shotgun sequence.
ACCESSION   DASTJP010000011 DASTJP010000000
VERSION     DASTJP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 22744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22744
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735730"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VFQ23_00280"
     CDS             <1..1496
                     /locus_tag="VFQ23_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9905032.1"
                     /translation="LSDPEPDVRFEAVAALSALSAPARGLTTYLTPILNDEDARVSTR
                     AAVSILKSHSLHGEWDKGDGDLITKAKFHLRNTAVLGDLEERLHAIAALGEWNDKEAF
                     DFLANELRDRGIEPAIRNAILASLFQINSQNSIPHLLEALKDPSVRGESARLLGMIGA
                     PVMEPVISKLGDEAFVDGTLLALQYLPVPPAKQIIDFARSSVSRAMEYDTLMGGVNSQ
                     IRNKNASADPSVSIDGHSRSEAMQLLGEALRNKSLQHGIRALRAIGLLGDRESMNMAI
                     ENLQARETGHLANVMEALESISAKWRDILQPLMQLWEEENHTDADINWERLLNDEDDW
                     IRDCAAFARSYGEVKMDASATLSLMDRILFFKRVPLFENLSPIDLKQAASIAEEEVFS
                     DGEELAHEGEVGDVMFIIVSGEVRVSSLQDGKDMEIARRRAGDVVGEMAIIGREPRMA
                     SLVAIGDVRVLCIDQKSFEGLLRERPDVSLSVIQVLNKRLKEASLKK"
     gene            1612..2721
                     /locus_tag="VFQ23_00285"
     CDS             1612..2721
                     /locus_tag="VFQ23_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9905033.1"
                     /translation="MKINHYLVPILAICLTISSCGQIPITSTPGNGTTTPGSEITPID
                     LASGHGVKGSWFELYFTDPTGPLSPQATGGVDGPLVEAIAGARLSIDAAAYSLSLNSV
                     RFALIDAHRRGVQVRVVMESTNMDRSDPQALIEAGIPVIGDNMDGLMHNKFMVIDKSE
                     VWLGSMNFTDSGTYDDNNNLMRIHSTKLAENYSLEFKEMFEDGMFGPNIAPRTPNPIV
                     TIDETRIENYFSPDDGVINALAMLLNGAEESIYFLTFSFTSNELGDIVRAKEEAGLTI
                     AGVMDEEQISSNQGTEYDPFKQAGIDVRIDGIPGQMHHKVFIVDEKIVVLGSYNFSQN
                     AELRNDENVIIVYNPIIAEQFILEFERVQKVARDD"
     gene            2711..3535
                     /locus_tag="VFQ23_00290"
     CDS             2711..3535
                     /locus_tag="VFQ23_00290"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET9905034.1"
                     /translation="MTTNLKLLVVFAHPDDESMGMGGTLAKYSAQGVDTYLICASRGE
                     RGWFGPKEENPGLSRLGQIRTQELENAAKELGIKEIQFLNYVDGEVDQADHAEVRAKI
                     VAHICRIRPQVIVTFPPDGNYGHPDHIAIGQFTSAAIICAADASYATSKNLSAHRVLK
                     LYYMVDSENLINFIAPFMSDMTFNVGGQIHQEIAWPEWMITTRIEMAEYCSTALRAVR
                     CHQSQLSTLGALAEVHDDAAVEVLAMQGTFYRAFSLVNGGRTLEKDFFEGLTQEAT"
     gene            3532..4341
                     /locus_tag="VFQ23_00295"
     CDS             3532..4341
                     /locus_tag="VFQ23_00295"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET9905035.1"
                     /translation="MTDSLKLLAVFPHPDDETLGLGSTFARYSAEGVETYLVCATRGE
                     RGWFDSEGPNPGLDGVAKIREAELTCAAEHLGLREVNFLDYIDGDVDQSDHKEIIGKI
                     VTQIRRIRPQVVVTFAPDGSYGHPDHIALSQFTMGALVCAADQEYIDSDEQISYTVSK
                     FYYMVDSVEGVRLANEAFGGISMDVDGVTRHHVGWEDWQITTRLDNTRYMDAVQKAIK
                     CHQSQLPGYGPISDWPLDELTKVFGKGYFYRTYSLINGGRKVESDLFEGLR"
     gene            4349..5530
                     /locus_tag="VFQ23_00300"
     CDS             4349..5530
                     /locus_tag="VFQ23_00300"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9905036.1"
                     /translation="MFIRWLMILSILALVSCQPGTSTSMPPTFTPQPTSIPTDTFTPS
                     QLPTELPAITPTIPASIIELDGAEVQPGFSLIKFADLYRPTAITFDAQGQMYVASQDG
                     NIYILSDDNGDGRADSRFTFGVGFYLPLGVTVHQSTGDVYVSHQGKITRLRDTDSNNR
                     ADNQVVFVDSLPFELHQNDNLEFGPDGWLYIGIGSICDACNDPDPRSASILRFNVDTA
                     DYEIFATGLRNPFDIAFHPITDELFATDNGRDDLGMDAPFEELNHIVQGGDYGYPNCW
                     NEHTVSGCESSHSAVAFFESHSSANGLDFYSGETFPDQYRSNAFVSIFGSWLKPNVQT
                     GIARVILTPEGNTYKGDTSWFVRFPIGVMPLPLLFGPDDALYVGDYVNDAIYRVSYGM
                     P"
     gene            complement(5537..8917)
                     /locus_tag="VFQ23_00305"
     CDS             complement(5537..8917)
                     /locus_tag="VFQ23_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9905037.1"
                     /translation="MASQNKSTLSEEREQVLLAIAALEEKRSLLGDQVVDVALASMRE
                     KLAGLAEEAGIIHEPQQRKLVTVLFADVSGFTAMAETMDHELVSSVINSLWSRVDKAI
                     LDHGGRIDKHIGDAVMGLFGTPTAREDDPERAIRAALQIQSEVRSWKKELAKLGTNQP
                     SQPRNIRLRIGINTGPALLGTVGTIGEYTAIGDTVNLANRVQSAAPLDGILISHNTFQ
                     HVRGIFDVTALEPITVKGKREPIRVYTVNGLNPRSFRMTTRGLEGVETRTIGREEELT
                     QLKAAFEQSVEQRQTALVNLVADAGLGKSRLLFEFGKWLDVQKHPVHLFKGRATQEMS
                     QIPYALLRHILSLEFNIQDTDRAAVARQKLERGILAFIRQKKKAILYAHFIGHLIGFD
                     FSKSSHLKGILGDSRQIRDLAFHYTTQLFAEIASDQAAVLFLEDIHWADKGSLDFFDH
                     LMSTQPDLPLFIVGLTRATLFEQRPDWGTAEIQNLRLNLLPLSESDCHRLIAEILKKV
                     PEIPTALTDLIIKKAEGSPFYIEELIKILIEGGVIVRGEETWTVEIDRLSDLKVPATL
                     TGLVQARLDSLNTNVRETLQQAAVVGRIFWTNVLENMSNPETQNVASSIAIFDKMNAL
                     RAKDLIYKYDESSSSEMPEYIFKNTILHDVTYESVLLKLRPIYHLQAAQGLIEISGER
                     VSEYAGRVGEHYERAGEWLEAAEWYARAGRQAQDTYEPDAAIDFYQKALSFLNQHGSP
                     DQIPQRLGICSRLGEVLNWKARYTDAVEIYELMLKIAEESGNLVAQSRALRGHATSLG
                     YLGDHRASLKSATSAETLARNANAHLDRAKALWTQGSAKFRLGDSHSASFLGEQALKI
                     ATDLDDRNEMARCLNLLGAVQYVSGDYEQAESYWTSALKIFEELGNKQQGMDLWSNLG
                     VSADGRGDYDTAFQRYNSALEIARETGYRDGEIVFLSNRGIEQVVLKNYAAAESDLRE
                     VIRMAGTTGSWCLPNTFNYYAEALLGLGRDQEAVYAAQQGLALSIEDGTPENIGGAWR
                     ALGIVAGKTDKTVRIKDKETRQSTDYDARTSFAKSEKVYAEAEIDGERARTLREWARY
                     ELKNGDKKQGRKLWKQARDLFDKLGAQLEVDRMKELPS"
     gene            9156..9692
                     /locus_tag="VFQ23_00310"
     CDS             9156..9692
                     /locus_tag="VFQ23_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905038.1"
                     /translation="MKRKFLSILTVFLLTTLLTSTVSAGGNIKLSGAPTSRSPLHLDG
                     YLTGVGGYPQGVTVELIGSGVPVTLCTNPGGNQAPGQNPPKIQTAGTDFIGPQDIQKN
                     GKAPVNVVVEDMIGTTLPGNQGGCPNNSWTAEITNIAWTDAVVLVTNNANGNLLLRVV
                     FTCFPAPGIPGAYDCRRV"
     gene            complement(9806..11164)
                     /locus_tag="VFQ23_00315"
     CDS             complement(9806..11164)
                     /locus_tag="VFQ23_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HET9905039.1"
                     /translation="MPQTQIACPRCRQMISANIEQLFDVTQDPQAKQRLLGGVSNTAR
                     CPHCGYQGRLATPIVYHDNDKELLLTYFPPELSVPINEQEKIIGPLIKQVMDRLPPEK
                     RKGYLLNPTPNLTYESMVQVILGKDGITPEMLKEQQERVSLVERLLQVTSNDVRSELI
                     KQNAHLFDEQFFALFSRLAQSAAASGQEPVARAMVDVQKQLLEETEFGRGLKASVGEL
                     EAAQKSLQELGQGLTREKLLELVLASPNDARLQAYVSLARAGMDYQFFQLLTERIEKA
                     SGEEKAAFESIREKLLEFSNELDKQLEARYKQAQEFIESLLKQDDVVQAVHDNLDGFT
                     QDAVDIVNQMLRQASEKNDYERMGKLQKMMEVLRQVSTPPEVGFIEQLLDAPDASSLE
                     KMLEENQALVNDQFMEALIGLVAQVDAAAGQGNTEAKALGDKLNIIYKTALKYSMKKN
                     MA"
     gene            complement(11261..13069)
                     /gene="pepF"
                     /locus_tag="VFQ23_00320"
     CDS             complement(11261..13069)
                     /gene="pepF"
                     /locus_tag="VFQ23_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET9905040.1"
                     /translation="MANKILPRNKIDKKSTWNAESVFPSAEAWEKEATKIVDDIAKAK
                     QYQGRLSESAAILLEGLNVSEQLILRAYKLYMYASFSYAVETTNQQAAGLLGKAQAIY
                     GQVASAIAYIQPELLAVGKSRLDEWMSENKKLAVYRHNFEDLFRQQAHVRSAEVEEIL
                     GMVLDPFQGAFNSSSMLTNADFKFKPIKDSKGHTIDVTQGNYDSKLMHLPDRKARKAA
                     YETYMDKYLEHKNTLAANLETSIKANVFNMRARKHESTLSASLFNLNIPTDVFHNLIN
                     TFKKNLPVWHRYFDIRRKALGLKKLAYYDMWAPIAKKRVKVPYEEGVDMICKSLAPMG
                     KEYVETVRRGCLKDRWVDVYPNQGKSNGAFSSGAPGTHPFIMMSYADEVSSMSTLTHE
                     LGHSMHSYLTWKNQPFVYTGYSLFVAEVASNFHQAMMRGHLLNTVKDKNFQVALIEEA
                     VGGNFFRYFFQMPTLARFELETHQRSERGEPLTADSMIELMADLFGEGFGPNFDLDRN
                     RVGMTWATFQHLFADYYVYAYATGISGAHALAGRILRGEPNAVEDYLGFLKSGSSDYS
                     LNVLKKAGVDMTSPQAVEETFAVMESYVERLEKLIG"
     gene            complement(13191..14723)
                     /locus_tag="VFQ23_00325"
     CDS             complement(13191..14723)
                     /locus_tag="VFQ23_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905041.1"
                     /translation="MLPDFRVRQRDYLLEISRALTQELDLEKLLTRILGIAIEMLAGQ
                     AGIIALKEQEGWRVAAAHGIAPAFLSYLTPLLAEENVRELDVRELNRMLKELTYTATQ
                     GLLNGTGLRLTTHGQIIGVIFIFRTYPDLFTPNDRVLLQSFADQAAIAMYNAQLYGQV
                     SYEKQRLDALLDSAADGILILNADLTIERVNDAFEIIYGKTHDDLAGLPHDQIIQWAG
                     NPQGSTLTEAIANGWPLTPNATLYVEGDLERDPSPPLPVGVTYAPLLSPEDKLRNVIV
                     SVRDITHFRTADEIKATFISIVSHELRTPVALIKGYASTLRRDDAKWDKHTISDSLAV
                     IEEEADRLSRLVDDLLDASRLQAGGLSLNRADVSLPNLAQRVSERFSPQLKNHTIITD
                     FPEKFPVVLADDTRIEQVIVNLVSNSLKYAAEGEIRISGKVHPEQVVVCVSDEGTGIE
                     AKDLPHIFDRFYRSTKAVKQTKGAGLGLYLARAIVEAHGGRIWADPKPDSGARICFSL
                     PR"
     gene            complement(14848..16176)
                     /locus_tag="VFQ23_00330"
     CDS             complement(14848..16176)
                     /locus_tag="VFQ23_00330"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HET9905042.1"
                     /translation="MNKLNLPGPKARALIERDQKVISSSYPRAYPFVMDYGKGVEVWD
                     ADGNRFIDFMAGIAVLSTGHANQRVVKAVQDAAEKFLHISSDFYHEGWIRLSEKLDEI
                     APFEETAKTFLTNSGTEAVETAIKLAKFHTKRHNFIGFFGAFHGRTMGSVAFTASKPH
                     YHRGFYPLMPGVTHVPFPDAYRPILHRKLGEDYGETVVRFIEEEIFDHNVPGDEIAGI
                     LVEPIQGEGGYVVPPDGFFPALRKLCDKYGIMMIADEVQSGMGRTGKWWAIEHFGVEP
                     DIVTSAKGIASGLPLGACIARESVMTWPKGVHGNTYGGNPIACAASLATIELLEESYL
                     QNAADVGQYAIDALQEIQVRHTSIGDVRGKGLMIGIEFVKDKNTKEPNEEIRNMIEQM
                     GFEHGLLLLGCGKSTIRLAPPLCITKKEMDEGLEIFEKVITMAEHEQGLR"
     gene            complement(16405..17337)
                     /locus_tag="VFQ23_00335"
     CDS             complement(16405..17337)
                     /locus_tag="VFQ23_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9905043.1"
                     /translation="MSLTAAVDIGGTHIRVATYEPNSTEPTAHHRTRSLANEPGVFGR
                     LVEAIESVWEKDNVRAIGIASPGPLDPHTGTILATPNIAEWKNFPLAPNLSKHFGVPV
                     FLDNDANMAAFGEWQYGAGQGHHDLIYITISTGVGGGVICNDRLLQGFHGMGAELGHM
                     IIDEHGPICGCGFYGHVESFSSGTGIARYVSEQIKAGTTSSLQVDTNPTAAQVAGAAQ
                     VGDPLAIAAFERAGHYLGIAMANYLAIFNPSILIFGGGVSQVGDLLFKPFEESLKKHT
                     FHPHYLDGLVITRAALGDNAGLMGALAWARLKAK"
     gene            17433..18761
                     /locus_tag="VFQ23_00340"
     CDS             17433..18761
                     /locus_tag="VFQ23_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET9905044.1"
                     /translation="MDSTQTRRRIQQEMGRLYRWLLPGMGVKRWMLLILAGLTMLGIS
                     LAIFLYEIYDSGLPGQALSNILSGLSLSFLPRIVRVLIFGCLGLGLLISGIGGLNRAL
                     LKPFIGPGKSVVDELANYRRRDRGPRIVTIGGGHGLATLLRGLKSYTRNLTAVVTVAD
                     DGGSSGRLRETYGILPPGDIRNCLAALSNDEQMLTQLFQYRFSSTDDELEGHSFGNLF
                     ITALADITGSFEGAIAESGKVLSVSGRVLPSTLHDVRLVADMELPNVPIQVRVQGESR
                     IPKMAGQVRRLWLEPSDAPAFPPVLKAILNADMIVVGPGSLYTSLLPNLLVKDVLGAI
                     HASRALKVYISNIATQSGETDMYTCYDHVRALEEHLGNNVFDVILCNDNYEGQLNADS
                     QWVLADEKTLSDYRSYMTDLTDKGHPWRHDSLRLAKTLIEILDEYTGPLE"
     gene            18852..19862
                     /gene="gap"
                     /locus_tag="VFQ23_00345"
     CDS             18852..19862
                     /gene="gap"
                     /locus_tag="VFQ23_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9905045.1"
                     /translation="MAVKVGINGFGRIGRLVFRAIKERHPDEIEVVSVNDLFDAATNA
                     HLLKYDSTYGKYPGTVEAKDGKLVIDGKTVNVTAERDPGAIKWKDMGVDIVIESTGLF
                     TDATKAKAHIDSGGAKKVIISAPAKNEDITIVLGVNQEKYDPAKHNIISNASCTTNGL
                     APVAKVLNDAFGIDKGVLTTVHAYTNSQKLQDVGAKDPRDARAAAQNIVPSETGAAKA
                     VGLVIPELKGKFGGMAFRVPTPTVSVVDFTATLNKEASKEEIRDAMLEYAQGPMKGIL
                     DVTDEPLVSTDLRGTNFSSVFSAMDTLVIGNMVKVVAWYDNEWGYACRTADLTALIAQ
                     SL"
     gene            19936..21108
                     /locus_tag="VFQ23_00350"
     CDS             19936..21108
                     /locus_tag="VFQ23_00350"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET9905046.1"
                     /translation="MNKKTVKDIDLKDKRVLMRVDFNVPMKDGKVTDDKRIRASLPTI
                     KYILDQGASLILMSHLGRPKSASDSEFSLRPAADVLSSLLELPVKMAPDSVGSEVESM
                     VKELQPGEVLMLENTRFHPEEEKNDLEFAKQISVLGEIYVNDAFGSAHRAHASTEGVA
                     RFLPAVSGFLMEQELEYLGRAVANPEHPYIAILGGAKISDKIDVVESLLSKADKLIIG
                     GGMANTFLAAQGLNMQDSLVETSSLDTAKSILEKSGDKLILPVDAVIADKFEETANSQ
                     VVDVDKIPAGWRMLDVGPQTLELYKRALDGAKLIVWNGPVGVFEMPKFAEGTFALAKL
                     LAESGATTVIGGGDSASAVKKAGVAKQMTHVSTGGGASLEFLEGKELPGVAALMDK"
     gene            21288..>22744
                     /locus_tag="VFQ23_00355"
     CDS             21288..>22744
                     /locus_tag="VFQ23_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9905047.1"
                     /translation="MPFAVGENVGPYRIIEQLGQGGMATVFKAYHASLDRYVAIKALH
                     PAFNQDVTFEARFQREARVVARLEHPHIVPVYDYAEFEKRPYLVMKFIEGDTLKAKLD
                     QGSLSSDDVTRIVDAVGSALSYAHRQGVLHRDIKPSNVLVAKDGQIYLADFGLARIAQ
                     SGESTLSSDMIMGTPQYISPEQAMGVKELDERTDLYSFGVMLYEMVVGKVPFNADTPF
                     SVIHDHIYSPLPLPHTVNPDVSESVERVLLKALAKERNDRYENVAQMVGAFTAARAEA
                     NLTGRETPIQVSEPLSPVETIVPKSEPVQTLIEPDASNMKATPVAETRRSSSIWLLVG
                     LAIVLCVGALVFARNNRLFARFINASRSPTTTPEVFSISTSIPNLQLFTPSSGQDLPP
                     DVIEAQQRVNENPGDPNARLDLAFAFWNAGMPGATYETLGEVVRLAGSENEPFYMDAG
                     NAFADQEGWLPAAFLYYQVVKVRSQTSEGVPDTIR"
BASE COUNT         5548 a         5579 c         5693 g         5924 t
ORIGIN      
        1 ctctttcgga ccccgagcct gacgtccgtt ttgaagctgt agctgcactt tcggctttat
       61 cagcccctgc gcgcgggcta acgacctacc tcaccccgat tctgaatgat gaggacgccc
      121 gggtaagcac acgggctgcg gtgtccattc ttaagtctca ctctctccac ggcgaatggg
      181 ataaaggaga tggcgatctc atcaccaaag ctaaatttca tctccgcaat acagccgtat
      241 tgggtgatct cgaagagcgt ttgcacgcca tagctgcgct tggagaatgg aatgataaag
      301 aagcctttga ctttcttgcg aatgaactgc gtgatcgagg tattgagccc gccatcagaa
      361 atgcgattct ggcatctctc tttcaaatca attcacaaaa ctctatccct cacttactgg
      421 aggcgttaaa agatccatct gtgcgtgggg agtcagcaag attgctcggg atgatcggcg
      481 cgcctgtgat ggaaccggtc atctcgaagt tgggcgatga agctttcgta gatggaacgt
      541 tgttggcgct tcaatatcta cctgtgcctc cagccaaaca gatcattgac tttgctcgtt
      601 cgtctgtgtc ccgcgcaatg gagtatgata cattgatggg tggagtcaac tcacagatca
      661 gaaataaaaa tgctagcgcc gacccgagcg tgtcgataga cgggcacagt cgaagcgagg
      721 cgatgcagct tctgggcgaa gctttgcgca ataaatctct ccagcatggc attcgtgcct
      781 tgcgcgcgat tggccttctg ggtgaccgcg agtcgatgaa tatggcgatt gaaaatttac
      841 aagcacgcga aacaggtcac ctcgcaaatg tcatggaagc tttggaatcg atcagtgcca
      901 aatggcgtga tatccttcaa cccctaatgc agctatggga agaagaaaat catactgacg
      961 ccgacatcaa ttgggagcgg ttgctgaatg acgaagatga ctggattcgg gattgcgcgg
     1021 cgtttgcgcg aagctatgga gaagtaaaga tggatgcctc tgcgacttta tctttaatgg
     1081 atcggatatt gtttttcaag cgtgtaccgt tattcgaaaa cctttcccca atcgatctga
     1141 aacaagctgc ttcgattgcc gaagaggaag tgtttagcga cggcgaggaa cttgcacacg
     1201 aaggcgaagt gggagacgtg atgttcatta tcgtcagcgg cgaagtgcgc gtatcctcat
     1261 tgcaggatgg gaaggacatg gaaattgcac gccgcagagc aggcgatgtg gtcggtgaaa
     1321 tggccatcat tggacgggaa ccgcgcatgg cctccctggt tgctatcggc gatgtgcggg
     1381 tgctctgcat tgaccagaaa agctttgaag gactcttgcg cgaacgcccc gatgtcagct
     1441 tgtcggtgat acaagttctg aataaacgct tgaaagaagc gtctttgaag aaataaacca
     1501 tctgataacc aaagaatttt tgagagataa attttcttac gggtggtttc gatatgctcc
     1561 tggttatcac tcgacgctac ttaaaaaccg aagtaaccac ttaaggattc catgaaaata
     1621 aatcattatc tcgtgccgat acttgccatt tgcctcacga tctcctcttg cggacaaatc
     1681 cccataactt caaccccagg aaatggtaca acaacgccag gaagtgagat tacaccaatt
     1741 gacctggcgt ccgggcatgg tgtaaaaggc tcatggttcg aactttattt cacggatccc
     1801 acaggcccat tatctccgca ggccactggt ggagtcgatg gtcccttagt agaagcgatt
     1861 gctggggcac ggctaagtat cgatgctgct gcctacagcc tcagtttgaa cagtgtccgc
     1921 tttgcgttga tcgatgccca tagacgtggg gtgcaagtgc gagtggtgat ggaaagcacg
     1981 aacatggatc gttctgatcc tcaggccctc atcgaagcag gcattccggt cattggcgac
     2041 aatatggacg gattaatgca caacaagttc atggtgatcg acaaatcgga agtctggttg
     2101 ggctcgatga acttcaccga ctctggcacc tatgacgata acaataacct gatgcgtatc
     2161 cactccacga agctcgcaga aaattactct ctcgaattca aggaaatgtt cgaagatggc
     2221 atgtttggcc cgaacattgc cccgcgtacg ccaaacccca ttgtcacgat tgatgaaaca
     2281 agaattgaaa actatttttc gccggatgac ggagtgatca atgcgctggc tatgttgcta
     2341 aatggtgcag aagaaagcat atactttctt acgttttcat tcacgtccaa tgaactgggc
     2401 gacatcgtcc gtgcgaagga ggaggcgggt ctcaccattg cgggtgtgat ggatgaagaa
     2461 caaatttcat cgaatcaggg cacggaatac gatccgttta agcaagccgg aatcgatgtc
     2521 cgaatcgacg gtatacccgg gcagatgcat cataaagtgt tcatcgtgga cgaaaaaatt
     2581 gtggttctgg gttcctataa tttttcgcaa aacgccgaac tgagaaacga cgagaacgtc
     2641 attattgtgt ataacccaat cattgcagaa cagttcatct tggaattcga gcgcgtacag
     2701 aaagtggctc gtgacgacta acttaaaact cctggttgta tttgcccatc ccgatgatga
     2761 atcaatgggg atgggtggaa cgcttgcgaa atattctgct caaggagtgg atacctacct
     2821 gatttgcgcc tcacgcgggg aacgcggctg gttcgggccc aaggaagaga atccaggctt
     2881 gagccggctc ggccaaatcc gcacccagga gctcgagaat gctgcaaagg agctgggtat
     2941 taaggaaatt cagtttttaa attatgtgga tggtgaagtc gatcaggccg atcatgcaga
     3001 agtcagagcc aaaattgtgg ctcatatttg ccgcattcgg ccacaagtta ttgtcacgtt
     3061 tcctcctgat ggaaactacg gccatcctga tcatattgca atcggtcaat ttacaagtgc
     3121 ggcaatcatc tgtgcggcag atgcaagtta tgcaacttca aaaaacctct cggcacaccg
     3181 cgttctaaaa ttgtattaca tggtggattc tgagaatctc attaacttta ttgccccatt
     3241 tatgtctgat atgactttta atgtgggtgg ccagatacac caagaaatcg cctggccaga
     3301 gtggatgatc acaactcgca ttgaaatggc agagtattgc tcaaccgcgc tgcgcgcagt
     3361 tcgatgccac cagagtcaat tatccaccct tggagcgctg gctgaagttc atgacgacgc
     3421 tgcggtagaa gtccttgcaa tgcagggaac gttttaccgc gcattcagtt tggtcaatgg
     3481 tgggagaact ctggaaaaag acttttttga aggacttaca caggaggcaa cgtgactgat
     3541 tctttgaaac ttctggctgt ctttccacac cccgacgatg aaaccctggg tttggggtct
     3601 acgttcgcgc gttattccgc agaaggcgtg gaaacttatc ttgtttgcgc aacacgcggc
     3661 gagcgtggtt ggttcgattc cgaaggacca aacccgggac ttgatggtgt ggctaaaatt
     3721 cgcgaggctg aattaacctg cgcagcagaa catctcggtc ttcgtgaggt gaacttcctc
     3781 gattacattg atggagatgt cgatcagtca gatcacaagg aaattatcgg caagatcgtt
     3841 acccaaattc gtcgcatccg gccgcaggtg gttgtcacct tcgcacccga tggaagttat
     3901 gggcatccgg accatatcgc gctgtcacaa ttcacaatgg gcgcccttgt gtgtgcagct
     3961 gatcaggaat atatcgattc agatgagcag atttcttaca cagtttcaaa gttttattac
     4021 atggtagatt cagtagaggg tgttcggtta gcaaacgaag cttttggcgg tatcagcatg
     4081 gatgtagacg gggtcacgcg tcatcatgtt ggctgggaag attggcagat cacgacacgc
     4141 cttgataaca cccgatacat ggatgctgta caaaaggcga ttaaatgcca tcaatctcaa
     4201 ttgcccggct acggtccaat ctcagattgg ccgttggatg aattaacaaa ggtttttggt
     4261 aagggctatt tctatcgaac ttatagcctt attaacggtg gaagaaaagt tgaatcggat
     4321 ttgtttgagg gattaagata ggaaactcat gttcattcga tggttaatga tattgagcat
     4381 cctggcgcta gtctcttgtc agcctgggac aagcacgtca atgccgccga cttttacacc
     4441 acaacctaca tccatcccta cagacacttt caccccgtca caattaccta cggaactccc
     4501 tgccattacg ccgactatcc ctgcttccat catcgaactg gacggcgcag aagtgcagcc
     4561 gggtttttcg ttaatcaaat ttgcagatct ctatcgccct acagccatta cgtttgatgc
     4621 acagggacag atgtatgtgg cctctcaaga tggcaatatc tatattctga gcgatgataa
     4681 tggcgatgga cgcgctgact cacgctttac cttcggggta gggttctacc tgcctttggg
     4741 ggtgacagtt catcaatcga ctggcgatgt ctatgtatca catcagggaa agattacccg
     4801 tctccgtgat accgatagca ataaccgggc agataaccag gtggtttttg ttgatagcct
     4861 tccttttgaa ttgcatcaaa acgacaatct tgaatttggt ccggatggat ggctgtatat
     4921 cggtattggt tccatttgcg atgcatgcaa tgatccggac ccgcgcagcg cctcaatttt
     4981 gcgattcaat gtagacacgg ctgactacga aatttttgct accggtttgc gcaatccatt
     5041 tgatattgcg tttcatccca tcactgatga gctgtttgcc accgacaatg gtcgtgatga
     5101 tctgggtatg gatgccccat tcgaggagtt aaatcacatt gtccagggtg gcgattacgg
     5161 atatccaaat tgttggaatg agcacacggt gtctggatgt gagagctctc attcggctgt
     5221 cgcgttcttt gaatctcatt cctccgcaaa cgggttggat ttttacagcg gagaaacgtt
     5281 ccctgatcaa tatcgcagca acgcctttgt atccattttc ggttcatggc ttaagcccaa
     5341 tgtgcaaacc ggcatcgcaa gagtgattct tacgcctgaa ggaaacactt ataaaggcga
     5401 tacttcctgg tttgtgcgtt tcccgatagg cgtcatgcct ttacccctct tgtttggccc
     5461 tgatgacgcg ctgtatgtcg gagattatgt caacgacgcg atttaccgcg tcagctatgg
     5521 aatgccttga ccgaagttag gacggtagtt ccttcattct atccacttcc aattgtgcgc
     5581 ccaatttgtc aaagaggtcg cgggcctgct tccataactt tctgccttgc tttttgtccc
     5641 catttttcaa ttcgtacctg gcccactcgc gtagtgtgcg cgctcgttca ccatcgattt
     5701 cagcttctgc gtaaactttc tcgcttttgg caaacgaagt tcgagcgtca taatcggttg
     5761 actgacgggt ttccttatcc ttaattcgaa cggtcttatc agtttttccg gctacgattc
     5821 ccaatgctcg ccaggcaccc ccaatatttt ctggagttcc gtcttcgata ctcaatgcaa
     5881 gaccttgttg tgcagcataa actgcctcct ggtcccgacc aagccccagc aacgcttcag
     5941 cataataatt aaaggtgttg ggaaggcacc acgaacctgt cgtccccgcc atccgaatga
     6001 cctcacgcag atcagattcg gctgctgcat aattcttgag cacgacctgt tcgattcctc
     6061 gattggaaag aaaaacgatt tctccatcgc gatacccggt ttctcgggca atctctaaag
     6121 cgctattgta tctctggaag gcggtgtcat agtcacctcg cccgtctgcg ctgacaccca
     6181 gattgctcca taaatccatt ccctgctgtt tattgccaag ctcctcaaag attttcaacg
     6241 cgcttgtcca atagctctct gcctgctcgt aatcacccga tacatattgc accgccccca
     6301 gcagattcaa acatcttgcc atttcgttac gatcgtccag gtcggtagcg atcttcaaag
     6361 cctgttcgcc caaaaacgaa gcgctgtgcg aatcgcctaa cctaaacttg gcggagcctt
     6421 gggtccacag ggcttttgcc cggtccaagt gcgcattcgc atttcgcgcg agagtttcag
     6481 cgctggtcgc acttttcaga gaagcgcgat gatcgcctaa atagcctaaa gaagttgcat
     6541 ggcctcgcag ggcacgtgac tgggcgacaa ggttcccgct ctcttctgca atctttaaca
     6601 tcagctcata aatctcgaca gcatcggtat accgtgcctt ccagttcaag acctctccga
     6661 gtctggaaca tattccaagc ctttgaggaa tctggtcggg gctgccatgt tgatttagaa
     6721 acgacaatgc cttttggtaa aaatcaatgg ctgcatcggg ttcgtacgta tcctgcgcct
     6781 gtctgcctgc acgggcatac cattctgcag cctccagcca ctcgccagca cgttcatagt
     6841 gttcacccac gcgtccggcg tactcgctta cgcgttcacc gctgatttca atcagaccct
     6901 gcgctgcctg caagtgataa attgggcgta atttcagaag cacgctttcg tatgtcacat
     6961 cgtgcagaat cgtgttctta aagatatatt ccggcatttc agacgaggat gattcgtcat
     7021 acttgtaaat taaatctttg gctctcaagg cattcatctt gtcaaagatc gcaatggagg
     7081 atgccacatt ctgagtttcc ggattactca tgttttcaag tacattcgtc cagaagatgc
     7141 gacccacgac agctgcctgt tgcaaagttt cgcgcacatt tgtgttgaga ctatccaatc
     7201 gcgcctggac caggccggtg agagtggcag gcaccttcaa atccgagagt cggtctatct
     7261 ctactgtcca ggtttcttct cctcgaacaa tcactcctcc ctcgatcaaa atcttgatca
     7321 gctcttcgat atagaacgga ctgccctcag cctttttgat gatgagatct gtaagtgcag
     7381 ttgggatctc gggaactttt ttcagaattt ctgctatgag tcggtggcag tcgctttcag
     7441 agagcgggag cagattcagg cgcaagtttt gaatttcagc tgttccccaa tcaggccttt
     7501 gttcgaatag agtcgctctc gttaatccaa cgataaaaag aggcaggtca ggctgggtcg
     7561 acatgaggtg gtcgaaaaag tccagcgagc ctttatctgc ccagtgaata tcctcgagaa
     7621 acaggactgc ggcttgatca ctggcgattt cggcaaaaag ctgagtggtg taatgaaatg
     7681 caaggtcgcg aatctgacgt gaatctccga gaatgccctt aaggtgtgaa cttttggaga
     7741 agtcgaaccc aattaaatgc ccaataaagt gggcgtacaa aattgctttt ttcttttgcc
     7801 tgatgaaagc cagaattccg cgttctaatt tctggcgagc aacggcagct cgatcggtat
     7861 cctggatatt gaattccaga gacagaatat gtcgcaggag agcataagga atttgtgaca
     7921 tctcctgcgt ggctcttcct ttgaagaggt gaacggggtg tttttgtaca tcgagccact
     7981 tcccgaattc aaacagcagg cgtgattttc caagcccggc atccgcaaca aggttaacca
     8041 gagctgtttg tcgctgctcc accgattgct cgaaggcggc ttttagctgt gtcagctctt
     8101 cttcacggcc gatcgtccgg gtttcgacgc cttccagacc gcgcgtcgtc atccggaacg
     8161 accgggggtt taatccgttg actgtataca ctcgaattgg ctcacgcttg cctttgaccg
     8221 taatcggttc gagtgccgtg acatcaaata tgccacgcac atgctggaag gtgttgtggg
     8281 agatcagaat tccatccaaa ggtgctgcac tctgtactcg attggcgaga ttcacggtat
     8341 ccccgattgc ggtatattcg ccgattgttc caacggtccc taacagtgcc ggacctgtgt
     8401 taattccaat tcgtaaacgg atgttgcggg gttgagacgg ttgattcgtc cccagtttcg
     8461 ccaattcctt tttccagctg cggacttctg actgaatttg cagggcagca cggatggctc
     8521 gttcgggatc atcttcacgg gcagttggcg taccgaagag tcccatcacg gcgtcgccga
     8581 tatgtttgtc aatccgacct ccgtgatcga gaatggcttt gtccaccctg gaccacaatg
     8641 aattgataac gctgctgacc aattcatggt ccattgtctc agccatagcc gtgaacccgg
     8701 agacatcggc gaacaacaca gtgaccagct tgcgctgttg aggttcgtgt ataattccgg
     8761 cttcctcagc taacccggcc agcttttccc gcatggaggc cagggcaaca tccacgactt
     8821 gatctcccaa cagggatctt ttttcttcaa gagcggcgat agccagtaga acttgttcgc
     8881 gctcttctga caaagttgat ttattttgag atgccattta gaaccgttaa gaaggtgcca
     8941 caaagtgtaa ccttttcgtc attttatgtc atgacaataa aggtacaatt tcatcaatta
     9001 tcaggcattt ttttgttatc tgcaacatat aaattagtcg ataattcgga caacgggtgg
     9061 tgcaaaccag caagaaatgg aggcaaatct cgaagcacgt gattttcact ttcggtaaaa
     9121 cctacgaatc gtcacacaga catatgagga gacatatgaa aaggaaattt ctttcgatac
     9181 tgacagtttt tctgcttacg accctgctga cctccacggt gtcagcaggt gggaatatca
     9241 aactttctgg tgcacccact tccagatcgc cactccacct tgatggatat ctgacgggag
     9301 tgggagggta cccgcaaggc gtgaccgtag aactgatagg ctctggtgtt ccggtcacgc
     9361 tctgcaccaa tccgggcggc aatcaagctc ccgggcaaaa cccgcctaaa attcagaccg
     9421 ctggcaccga cttcatcggg ccacaagata ttcagaagaa tggcaaagcg ccagtaaatg
     9481 tggttgtaga agatatgatc ggaactaccc tacctggcaa ccagggagga tgcccgaaca
     9541 atagctggac ggcggaaatt acgaatatcg cctggacaga tgctgttgtt ctggttacca
     9601 acaatgccaa tgggaactta ctattacgcg tggtatttac ctgtttccct gcacctggca
     9661 ttcccggcgc ctacgattgt agacgggttt aatattttca gtgtattaag aaaacaaaac
     9721 tgggttccgc aaggaaccca gttttggttt gccgtgttct atttgagaga ggttgtatca
     9781 agcgataggc ttggaggact tatttttatg ccatattctt tttcatcgaa tacttgagtg
     9841 cggttttgta aatgatattc aacttgtcac ccagcgcctt ggcttctgtg ttaccctgtc
     9901 cggcggctgc atccacttga gcgaccaacc cgatcagtgc ttccataaat tgatcgttaa
     9961 caagtgcctg gttttcctca agcattttct caagggaaga ggcatcaggt gcatccaaca
    10021 gttgttcaat gaaaccgact tccgggggtg tagagacctg gcgcaggacc tccatcattt
    10081 tttgaagctt acccatccgc tcgtaatcgt tcttttccga agcctggcgt aacatttgat
    10141 tcacaatgtc gacggcatcc tgggtaaacc catccaaatt atcgtggaca gcttgtacca
    10201 catcgtcctg tttgagcagc gattcaatga attcctgcgc ctgcttgtag cgggcttcca
    10261 attgcttatc cagctcgttg ctgaattcca ataatttctc gcggatcgat tcaaacgctg
    10321 ctttttcctc gccggaggct ttttcgattc tttccgtgag caattggaag aattgataat
    10381 ccatcccggc gcgtgccaga ctcacgtatg cttgtaagcg cgcatcattc ggagacgcca
    10441 ggacgagttc cagtagtttt tcgcgcgtga gtccctgtcc aagctcctgc agggactttt
    10501 gagcggcttc cagttccccg acggaggcct ttagcccgcg tccaaattcg gtctcttcga
    10561 ggagctgttt ttgaacatcg accattgccc gggccacagg ttcctgacca ctggccgctg
    10621 cgctctgtgc caggcggctg aacaaggcaa agaattgttc gtcgaataga tgggcattct
    10681 gtttaataag ttcactgcgc acatcattcg acgtgacctg cagaaggcgc tccacgaggg
    10741 atacgcgctc ctgttgttct ttgagcattt caggcgtgat accgtctttg ccgagaatga
    10801 cctggaccat cgattcatag gtcaggttcg gggtagggtt gagcaaatat cctttgcgtt
    10861 tctcaggtgg caaacgatcc atgacttgct tgatcaacgg accaatgatc ttttcctgct
    10921 cgttaatggg cacacttaac tcaggaggaa agtacgtgag caagagctct ttgtcattgt
    10981 catgatacac aattggagtc gcaagccgtc cctggtaacc gcaatgggga cagcgcgccg
    11041 tattcgatac gcctcccagc aatctctgct tggcttgtgg atcctgggtg acgtcaaaga
    11101 gttgttcaat attcgccgaa atcatctggc gacatcgtgg gcaggcaatt tgtgtttgtg
    11161 gcattagtag acttccgatt tacgattttt tggatttgcg attgccgaac agacattcga
    11221 cgaaccgata atttgataac taaattgtga atctctcgaa ctaaccaatt aatttttcca
    11281 accgttccac ataactttcc atcacagcaa aggtctcctc tacggcttgc ggtgaggtca
    11341 tgtccacacc ggctttctta aggacgttca aggaataatc tgaggagccg gatttaagga
    11401 agccgagata atcttccacc gcatttggct cgccacgcaa tattctccca gccagagcgt
    11461 gagcgcccga gatcccagtt gcataggcgt agacgtagta atctgcaaac aagtgttgga
    11521 aggtagccca ggtcataccg acgcggttgc ggtccaggtc aaagttagga ccaaagccct
    11581 caccgaatag atcagccatc agctctatca tgctgtctgc ggtgaggggt tcaccgcgtt
    11641 ccgagcgttg atgagtctca agttcaaaac gtgccagggt aggcatttgg aagaaatagc
    11701 ggaagaagtt tccgccgacc gcttcctcga tcagagctac ctggaagttc ttatctttga
    11761 ccgtgttgag cagatgccca cgcatcatcg cctgatggaa gttcgacgcc acctctgcga
    11821 caaataacga atagcccgta taaacgaaag gttggttttt ccaggtaaga taagaatgca
    11881 tcgagtgccc aagttcatgg gtaagagtac tcatactgga cacttcatct gcataactca
    11941 tcatgataaa gggatgcgtg cctggagcac ctgatgagaa agcaccatta cttttccctt
    12001 gattcgggta gacatccacc cagcgatctt ttagacaacc tctgcggacg gtctctacat
    12061 attccttgcc catcggagca aggctcttgc agatcatatc cacgccttct tcgtaaggta
    12121 ctttcaccct tttcttcgca atgggagccc acatatcgta ataggccagc ttcttcagcc
    12181 ccagagcttt gcgcctgata tcaaaatagc gatgccagac cggcagattc ttcttgaagg
    12241 tattgatcaa attgtgaaag acgtctgtgg gaatgttcag gttaaaaagc gatgcggaaa
    12301 gcgtactttc atgtttgcgg gcgcgcatat tgaacacatt cgccttgatg gatgtttcca
    12361 gatttgcggc gagtgtgttt ttatgttcca gatacttatc catatacgtt tcataggccg
    12421 ctttccgagc tttgcgatct ggcaggtgca ttaacttcga atcgtaattt ccctgcgtta
    12481 catcaatcgt gtgacctttg ctgtctttga tgggtttgaa tttgaaatct gcgttcgtga
    12541 gcatgctgga ggagttgaac gcgccctgaa atggatcaag gaccatgcct aatatttcct
    12601 ctacctccgc agaacgaaca tgtgcctgct ggcggaaaag atcttcaaaa ttgtgacgat
    12661 aaaccgccag ctttttgttt tcactcatcc attcatccag cctgcttttt ccgacagcga
    12721 gaagctcggg ctgtatatat gcgatggccg aggccacctg accataaatt gcctgggctt
    12781 taccaagcaa gccggcggct tgctggttgg ttgtttcgac ggcgtatgaa aaactcgcgt
    12841 acatatatag tttgtaggcc cgcaaaatta attgttcaga gacgttcaga ccttccaaca
    12901 aaattgctgc actttcggac aggcgtccct gatattgttt tgctttcgca atatcgtcaa
    12961 cgatcttcgt ggcttccttt tcccacgctt cagcggaggg gaagacactc tcagcattcc
    13021 aggtggattt cttgtcaatc ttgttgcgag gcaagatttt gtttgccatt atcgttttta
    13081 tcctttttga tgggatttag ttttagactc tttgggagcg ggttccgtac aatgtgattt
    13141 taccaccctg ctgtcacaca tctcttttag gagagggcta ggggaaaatc tcaacgcggc
    13201 agcgagaaac agatccgggc gccagaatca ggtttggggt ctgcccagat gcgcccgcca
    13261 tgcgcttcaa caatggcccg cgcaagatag agtcccaagc cagcaccttt ggtttgtttt
    13321 acggctttcg tagatcgata aaacctgtcg aaaatatgtg gcaggtcttt ggcttcaatg
    13381 ccagtccctt catcgcttac acagaccaca acttgttccg gatggacttt tccactaatt
    13441 ctgatctcac cttctgccgc atatttgagc gaattagaaa cgaggttgac gataacctgc
    13501 tcaatgcgtg tgtcatcggc gaggacaacg ggaaactttt caggaaaatc ggtgatgatc
    13561 gtatgattct ttagttgtgg gctaaatcgt tctgagacgc gctgtgccag atttggcagt
    13621 gagacgtccg cccggttgag actaagccca ccagcttgca gccgtgaggc atctaataag
    13681 tcatccacca gcctggagag tcgatccgct tcttcctcaa tgacggccag cgaatcgctg
    13741 atcgtatgct tatcccattt cgcatcatca cgccgtagtg tggatgcgta gcctttgatc
    13801 agcgcgaccg gagtgcgtaa ttcatggctt acgatcgaaa tgaacgtggc tttgatttca
    13861 tcggcagttc taaagtgtgt aatgtcgcgt acgctgacaa taacatttcg aagtttgtct
    13921 tccggagaaa gcaaaggtgc gtatgtaact cccactggca agggtgggga tgggtcgcgt
    13981 tccagatcgc cttccacata aagggtcgca tttggtgtaa gcggccatcc gttggcaatg
    14041 gcttccgtca gcgtggaccc ctgcggattg cctgcccatt gaataatttg atcgtgagga
    14101 aggccagcaa ggtcatcgtg ggtcttgcca tagattatct caaaggcatc gttcacacgc
    14161 tcgatggtga ggtctgcgtt gaggatcagg attccatctg cggcggagtc cagcaacgca
    14221 tctaatcttt gcttttcata agacacctga ccataaagtt gcgcgttata cattgcaata
    14281 gcagcctggt cagcaaaaga ttgaagcagc acgcggtcgt ttggcgtgaa tagatcagga
    14341 taggttcgaa aaataaaaat gaccccgatg atttgtccat gagtggtgag ccgcagacct
    14401 gtgccattga gaagtccctg tgtagcggta tacgtcagtt ctttcagcat gcggttgagt
    14461 tcgcgcacat ccaactcgcg cacgttttcc tctgccagca gaggggtgag gtaactcaag
    14521 aaggcaggtg caattccatg tgcggccgcg acgcgccagc cttcctgttc tttcagcgcg
    14581 ataatcccag cctgacctgc gagcatttca atggctatgc ctaaaatgcg cgtgagcagt
    14641 ttttcaaggt caagttcctg tgtgagcgca cgagaaatct caagcaaata gtcgcgttga
    14701 cgtacacgaa aatcggggag cataaaatca ttttaacgta cgagtataag agttgtgtaa
    14761 aaatattctg ccagcaatgc attgctggca gaagtattta acagaattag cgaagaagac
    14821 gcgttgtgtt caacatgtca acaagtttta tcttaaccct tgttcgtgtt ctgccatagt
    14881 gatcaccttt tcgaagattt cgagtccttc atccatttct ttcttggtga tacagagggg
    14941 cggtgcaagg cgaatcgtgg acttcccgca tcccaacagc agcaagccgt gttcaaatcc
    15001 catttgctcg atcatgttgc gaatttcctc attgggttct tttgtattct tgtctttcac
    15061 aaactcgatg ccaatcatca atcccttacc gcgtacatct ccaatggaag tatgccgaac
    15121 ttgaatttcc tgcaaagcat cgatcgcgta ttgacccacg tccgccgcgt tttgcagata
    15181 ggattcttcc agcagttcga tggttgccaa agaagctgca caggcaatag gattacctcc
    15241 ataggtgttg ccatgaacgc ctttgggcca ggtcatgact gattcgcgtg caatacaggc
    15301 tcccagtggc aggccagatg caatgccttt tgccgaagtc acaatatccg gctcaacgcc
    15361 gaagtgttcg atggcccacc atttgccggt gcgtcccatt ccgctttgga cctcatcggc
    15421 gatcatcata atcccatatt tatcgcaaag tttgcggagt gccgggaaaa atccatctgg
    15481 cgggacgacg taaccgccct caccctggat cggctcaact agtatgcctg cgatttcgtc
    15541 gccgggcaca ttatgatcga agatctcttc ttcaatgaaa cgtaccaccg tttcgccata
    15601 atcttcaccc agtttgcgat gcagaatagg gcgataggca tcggggaagg gcacgtgggt
    15661 gacaccaggc attaatggat aaaacccgcg gtgataatgc ggcttactgg ccgtaaaagc
    15721 gacggacccc atcgtgcgcc catgaaatgc accgaagaac ccgataaagt tgtgtcgctt
    15781 tgtgtggaac ttcgccagtt tgatggccgt ctccaccgct tcggtgcccg aattggttag
    15841 aaatgttttt gctgtttctt caaatggcgc aatctcatcc agtttttcgc tgaggcgaat
    15901 ccagccttca tgatagaagt ccgatgagat gtgtaagaat ttttcagcgg catcctgaac
    15961 cgcttttacg actcgctggt tggcgtgccc agtcgaaagc accgcgatgc ctgccatgaa
    16021 atcaataaaa cgattcccgt cagcatccca cacctccaca ccttttccgt aatccatcac
    16081 aaacggatag gcacgcgggt aggaagacga aatcactttt tggtcgcgtt cgataagagc
    16141 ccgggctttg gggcctggca gattcaattt attcatacaa actccttgct ttggttctta
    16201 agacagatga tgcactgtgc aacactcgcg cgttactcgt tgcgaatgtt gactcagtgc
    16261 tgcgtttcgt aacactggcg gcgtcacgct tccgctcgcg cggaagtcga ttcagttatc
    16321 gactgcttaa gattgctcga tcggctgtcc accactttgt tgcaagccac ggcgggggag
    16381 gaaaaatcat ccagcgttct gtgatcattt ggctttcaac ctcgcccagg ccagcgcccc
    16441 catcagacca gcgttgtccc ccagtgcagc tcttgttatc acaagtccat ccagataatg
    16501 cggatggaac gtatgcttct ttaaactttc ttcaaatggc ttgaaaagca aatctcccac
    16561 ctgtgatacc ccaccaccaa aaatcaaaat agacgggtta aagatggcaa ggtaatttgc
    16621 catggcaatg ccaaggtaat ggcccgcgcg ctcaaacgcc gcgatcgcga gcggatctcc
    16681 cacctgggcc gcgcctgcca cttgcgcagc agtagggttt gtatctacct ggagcgaaga
    16741 tgtcgtccct gccttaattt gctcgctgac gtaacgcgcg atgcccgtcc ctgaagaaaa
    16801 agactctaca tgcccataga atccacatcc gcaaatgggg ccatgctcat caatgatcat
    16861 atgaccgagt tctgctccca tgccgtggaa gccttgcagg aggcggtcgt tgcaaatcac
    16921 gccgccaccc acgcctgtgc tgatcgtaat gtaaatcagg tcatgatgac cctggcctgc
    16981 gccatactgc cattccccaa aagcagccat atttgcgtca ttatccagaa aaacgggcac
    17041 cccgaaatgt tttgacaagt taggcgccag cggaaaattt ttccactccg caatgtttgg
    17101 tgttgccaga atcgtcccgg tgtgtggatc cagcggacct ggtgatgcaa taccgatggc
    17161 tctgacgttg tctttttccc aaaccgactc aattgcctcc acaagacggc caaacacccc
    17221 cggttcattc gcgagagatc gtgttcgatg atgcgctgtt ggttcagtgc tattgggttc
    17281 atacgtggcg acgcgaatgt gcgtaccgcc aatatccaca gctgctgtga gactcatgcg
    17341 ggcaattata accgacggca tcgttacgaa atcgacaacg gctcggcagt aatttagttg
    17401 acttcacttc tcaatactgc tattatcatt ttatggatag tacacaaacc agaaggcgaa
    17461 ttcaacagga aatggggcgt ctatatcggt ggcttttacc cggtatggga gtaaagcgct
    17521 ggatgcttct gattcttgca gggctcacaa tgctggggat cagcctggct atttttttat
    17581 acgaaatcta tgattctggc ttaccgggtc aagccttaag caacattctt tccggactct
    17641 ctttgagttt tcttccacgc attgtgcgcg tgctcatttt tggatgcctg gggttaggtc
    17701 tccttatctc cgggattgga ggcttaaatc gcgctctact caagccgttc atcggaccgg
    17761 gcaaatcggt cgtggatgaa ctggccaatt atcgtcgccg cgaccgcgga cctcgtatcg
    17821 tgaccatcgg tggcgggcac ggtcttgcca cgcttttacg agggctaaag tcgtatacga
    17881 gaaacctgac cgccgttgtc accgtggcag acgatggggg atcgtctggt cgtttacgtg
    17941 aaacgtacgg aattttgcct ccgggcgata tccgcaactg tcttgcggct ctatccaatg
    18001 acgaacagat gcttacccaa ttattccagt atcgttttag cagcacggac gatgaattgg
    18061 agggacactc ttttggaaat ttgttcatca ctgcgctggc tgatatcacc ggaagttttg
    18121 aaggtgcaat tgcagaatcg ggcaaagttt tgtcggtaag tgggcgtgtg cttccatcta
    18181 cattacatga cgtacgactg gtggcagaca tggaactccc caatgtccca attcaggtcc
    18241 gagtgcaggg cgagagccgc attccaaaaa tggcggggca ggtgcgacgt ctgtggctcg
    18301 agccaagtga cgcgccagca ttccctccgg tgctcaaagc catcctgaac gcagatatga
    18361 ttgttgtggg accagggagt ttatatacca gtttgctgcc aaacctgctt gtgaaggatg
    18421 tactcggtgc catccatgcc agccgcgcac tgaaggtgta cattagcaat atcgcaaccc
    18481 agtctggcga aaccgatatg tacacttgtt acgatcacgt ccgtgctctg gaggaacacc
    18541 tgggaaataa tgtctttgat gtgattctgt gcaatgataa ctatgaaggt caactgaatg
    18601 cagactctca gtgggtactt gccgatgaaa agaccttatc cgattatcgc agttatatga
    18661 ccgatttgac agataagggc catccatggc gtcacgattc actgaggttg gcaaaaactc
    18721 tgatcgagat cctcgatgaa tataccgggc ccttagaata atctttcgag tatctttcag
    18781 cgtttcataa agacgtatgt gataaaatca tctggtttaa ttcatcaaaa aaatcattac
    18841 ggaggtttga aatggccgtg aaagttggca tcaatggatt cgggcgtatc ggacgcctgg
    18901 tttttcgtgc aattaaggaa cgtcatccag acgaaatcga agttgtctcg gtaaacgatt
    18961 tgttcgatgc ggctacgaat gcacatttat tgaaatatga ttccacgtac ggaaagtatc
    19021 ccggcacggt ggaagctaaa gatgggaagc ttgtcatcga tggcaaaact gtcaatgtga
    19081 ctgctgagag ggacccgggt gccatcaagt ggaaagacat gggtgttgac atcgtgatcg
    19141 agtctacggg cttgttcacc gatgcaacca aagccaaagc ccatatcgat tcgggcggcg
    19201 caaagaaagt catcatttcg gcgcctgcga agaatgaaga tatcaccatt gtgttgggcg
    19261 tcaatcagga aaaatacgat cccgcgaagc ataacattat ctcgaatgcg tcctgcacga
    19321 cgaacggact ggcaccggtt gccaaagtat tgaacgatgc gtttggtatc gataaaggtg
    19381 tgttgaccac tgtccatgcc tataccaatt cgcagaagct acaagatgtc ggtgcgaaag
    19441 atcctcgtga tgcgcgtgcc gcagcacaaa acatcgtgcc atccgaaact ggcgcggcaa
    19501 aagctgtggg tctggtgatt cctgaactca aaggtaaatt tggcggtatg gcgttccgcg
    19561 ttcctacccc aacggtctct gttgtggatt tcactgcaac gctcaacaaa gaagccagca
    19621 aagaagaaat ccgcgatgcc atgctcgaat acgcacaggg tccaatgaag ggcatcctgg
    19681 atgtaaccga tgagccgctg gtttcaacgg atctacgcgg aacgaacttc tcgagtgtgt
    19741 tcagcgcaat ggatacgctg gtgatcggca acatggtgaa agtggtggcc tggtatgaca
    19801 atgaatgggg ctatgcctgt cgtacggctg acctgaccgc tttgatcgca caatcacttt
    19861 agtctctagt ccactactct cctggctaac aagttaacca ggagagtagt gacaattaga
    19921 cagggagaca aattcatgaa caaaaaaaca gttaaagata ttgatctgaa agataaacgc
    19981 gtactgatgc gcgtcgattt caatgtgccg atgaaagatg gcaaagttac ggatgacaag
    20041 cgcatcagag cctccttacc gaccatcaaa tatattctgg atcagggcgc ttcacttatc
    20101 ttgatgagtc atttggggcg acccaaatcc gcctcagact cggaatttag cctgcgcccg
    20161 gcagcggatg ttttgtcttc gctcctggaa cttcccgtta agatggcacc cgattcggtt
    20221 ggctccgaag tggagtcaat ggtcaaggaa ctgcagcctg gcgaagtgct gatgctggag
    20281 aacacgcgtt ttcaccccga ggaagaaaag aatgatcttg aatttgccaa gcagatatct
    20341 gtacttggcg aaatatatgt caacgatgcg tttggatctg cgcaccgcgc gcatgcctcc
    20401 acagaaggcg ttgctcgatt tctccctgcc gtgtcgggct ttttgatgga acaggaattg
    20461 gagtatctcg gtcgcgcggt tgcgaatcct gaacatccat acatcgcgat tctcggcggc
    20521 gcgaagatca gcgataagat cgatgtcgtt gaatccctgc tttcgaaggc tgataaattg
    20581 atcatcggcg gtggcatggc gaataccttt ttagcggcgc aaggtttgaa tatgcaggac
    20641 agtcttgttg aaacgtcgtc actcgacaca gcgaagtcta ttctggaaaa atctggcgat
    20701 aaattgatcc tgcccgtgga cgcggtcatt gcagataaat ttgaagaaac ggccaattca
    20761 caggtcgtgg atgtagataa aatccctgct ggctggcgca tgctggatgt ggggcctcaa
    20821 acgcttgagc tttacaaacg cgctttagat ggggcgaagc ttatcgtttg gaacggaccg
    20881 gtgggcgtct tcgaaatgcc aaagtttgca gagggaacgt ttgcactggc caaactactt
    20941 gccgaatctg gtgcaacgac ggtcatcggg ggaggggatt cggccagcgc ggtcaagaag
    21001 gctggggttg cgaaacagat gacgcatgtt tctacaggtg gcggcgcctc gctcgaattt
    21061 ttagagggaa aagaacttcc gggtgttgct gcgctgatgg ataaatagct atagttgtgt
    21121 cattgcgagg gcgttagccc gaagcaatct cctcacagtc gaggggattg cttcaccgca
    21181 acgaacggcg gctcgcaacg acaagttgtt tttcagcaat acatttttca cattctttcc
    21241 atttgttgta cgctataatc cggtaaattt gcacgcggag tcaatgaatg cctttcgctg
    21301 tcggcgaaaa cgttggtcca tatcgaatca ttgaacaatt aggccagggc ggtatggcaa
    21361 cagtgttcaa ggcctatcac gcctctctgg atcgttacgt ggccattaag gcgctgcacc
    21421 cggcattcaa tcaggatgtt acgtttgagg ctcgatttca acgggaggcg agagtcgttg
    21481 caagactcga gcatcctcat attgtcccgg tatatgatta tgcagagttt gagaaacgtc
    21541 cgtatctggt aatgaaattt atcgaagggg atacgctcaa ggcgaaatta gatcaaggtt
    21601 cattatcttc agacgatgta acaaggatcg tagatgcggt tggttcagcc ttgtcgtatg
    21661 cacacaggca gggtgtgctc catcgcgata tcaaaccatc caatgtgttg gtcgccaaag
    21721 atggacagat ctatctggca gactttggct tggcccgcat tgctcaatct ggcgaatcca
    21781 ccctttcgtc agatatgatc atgggtacgc cgcaatatat ctcccctgaa caggcgatgg
    21841 gcgtcaagga actggacgaa cgcaccgatt tgtattcgtt tggtgtgatg ctgtacgaaa
    21901 tggttgttgg gaaggttcct ttcaacgccg atacaccctt ctcggtcatt catgaccata
    21961 tttattcacc gctcccatta ccccacaccg taaacccaga tgtgtctgaa tctgtagaac
    22021 gggtcttgct aaaagcgctc gcaaaggaac gcaatgatcg ttatgagaat gtggctcaaa
    22081 tggtcggcgc gttcacagca gcccgggcgg aagcgaacct gaccgggcga gaaacaccga
    22141 tccaggtctc ggagccttta agtcccgttg aaacgattgt tccaaaatca gaaccggttc
    22201 agacacttat agagccagac gcttccaata tgaaagcgac tccagtagcg gagacgaggc
    22261 gatcgtcatc gatatggttg ctagtaggat tggccattgt cctttgtgta ggcgcgcttg
    22321 tattcgcgcg caacaatcgt ctcttcgcgc ggtttattaa tgcttcccgc tcaccaacaa
    22381 ctacgccaga agttttttca atatcaacat caataccgaa tttacaactc tttacccctt
    22441 ccagtgggca ggaccttccg cctgacgtga ttgaggcaca gcaacgggtg aacgaaaacc
    22501 cgggtgatcc gaatgctcgg ctcgaccttg cttttgcatt ctggaatgca ggtatgccgg
    22561 gggcaaccta tgaaacgctg ggagaagtgg tccggttggc aggatcagaa aacgaaccgt
    22621 tctacatgga tgccggtaat gcatttgcag atcaggaagg ctggcttccg gccgcgtttt
    22681 tgtactatca agttgtgaaa gtgcgttcgc aaacgagcga aggtgttcca gatactattc
    22741 ggga
//
LOCUS       DASTJP010000012         5691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1264330, whole genome shotgun sequence.
ACCESSION   DASTJP010000012 DASTJP010000000
VERSION     DASTJP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264330"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VFQ23_00360"
     CDS             <1..1176
                     /locus_tag="VFQ23_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET9905048.1"
                     /translation="RDIYMHPELSGQEQRTASIVADHLSQAGLEVRTGIGGNGVIGLL
                     QGQKEGPVIAWRADMDAFAIPDILDVPYKSSVPGVKHVCGHDVHTTIGIGIAQVLALL
                     QDEIAGSIKFIFQPAEETATGARAMIHAGALSNPTPEVIFGLHTAPLPVGQIGCVPGM
                     ILPGIAKFEITLKSKNESVERLRDVAENCVAALGCLSSLRFPDSEELSGFLHAMETGE
                     NKALTQFILVSCWQESGGILKQNTIQLQGLVRAANEHLEEHAKLMIRKTLDRIVGELP
                     VDYNLQWLEQLSLPPAINNESLERQLRPAIAAMIGATNTVELRAPFPFNSEDFSLYQQ
                     QLPGVFYWLGVANLAKGIIGVPHLPNFDVDEECLVVGTKTMAHLLLHYFDSCLGIRH"
     gene            1956..4742
                     /locus_tag="VFQ23_00365"
     CDS             1956..4742
                     /locus_tag="VFQ23_00365"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9905049.1"
                     /translation="MQISDPLIHTKLCPPFIRPALVPRPRLQEQMVQGLRGPLTLIIA
                     PAGFGKTTLVASCLADCVTPVAWLSLDKSDNEVGQFLNYVIAALQEADNTIGGEAAQL
                     MAGRQQASPELVLTSLINDLDSARGEITLVLDDYQFVCNQAVHEQLAFLLEHCPPTFH
                     LVIATRSDPPLPLARLRARGQVVELRAVDLSFTEPETAQFLNDIMGLCLDVRSVTALA
                     ERTEGWIVGLQMAALSMRDRKDVAGFIEGFSGTNRYILDYLLEEVLVSQPPEIQHFLL
                     YTSILERLSASLCDAALANNQPTKREGDDASTGGALFLGSSTSILEYLERANLFLIPL
                     DDERIWYRYHHLFADLLRTQLQKLLGAQGVAQLHIRAADWHGQNGSLVDAIHHASMAS
                     DEERVERFIEQNYMELVSRGEQSWLRSWTGKLSKERVYRRPWLCIYEAYSHSWFGELD
                     QAEVFLDAAEKRIRSDMSVPDAQAMLGHLTYIKSRVTAMRGDLPRAIEFNLAAREYLQ
                     VSNLALQLDLGITLGYLYFLNGDYSHASQFLNETIRSGRAVGAILNTVAGYCIMARLH
                     TVEGRLNQSYEFYIQAAQWVHEVGGQHLGASSLIEVGIADVLCERNDLDAALTHMKQG
                     LALLPWWGKPDDLALAYCTLVRLHLAQANTSAASEAMEKATQVIQTSGLFPEAPRAVE
                     LARVKLWLTQGNVQAASHWAATLEQRFGSHDPFRFEEEVTYFAQARVFIALHKLDEAI
                     SLLSRLEQIARSAQRMGRVIEILLLEALAMQEKGDLENAVLALTKCLTLAEPEGYRRA
                     FLDEGQPVQLLLAQWLAHTSASPLRDYAVRLLAQFDAEPRAIQMAQEKTSPAGGPVEP
                     LSQREREVLHLIALGKSNQEIARQLIVSTGTVKAHTASIYRKLDVANRTQAAARARQL
                     GILPYSPFAGKPCK"
     gene            4915..5166
                     /locus_tag="VFQ23_00370"
     CDS             4915..5166
                     /locus_tag="VFQ23_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905050.1"
                     /translation="MSNSGKWLRVRVTDLRTGASKANVKIPVSLADFGMKMAASFAPS
                     AIDDLDMNEILTAMKSGGEEKFVEIEDEEKDEHIEIFIE"
     gene            5219..5455
                     /locus_tag="VFQ23_00375"
     CDS             5219..5455
                     /locus_tag="VFQ23_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905051.1"
                     /translation="MPEYYEIKIKGHLDERWSEWFAGLKLVYLEGDVTQFSGLLPDQA
                     AIYGVLERMRDLNLTLISVNHGGPSTQDCDKEKA"
     gene            5452..>5691
                     /locus_tag="VFQ23_00380"
     CDS             5452..>5691
                     /locus_tag="VFQ23_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905052.1"
                     /translation="MNKILLRIGGVINLLFFLFHLAMVKPLGEILAPLSPDIGATVST
                     LNIHVAFTLLIFAYLAIFQWHDLLTTRLGQITAIAI"
BASE COUNT         1417 a         1396 c         1489 g         1389 t
ORIGIN      
        1 cgagatattt acatgcatcc agaattgtcg gggcaagagc agcgaacagc aagcatcgtc
       61 gctgaccatc tgtcccaagc cggactggag gtcaggacag ggatcggggg caatggggtg
      121 attggtttac tgcaaggaca aaaagagggg ccggtgatcg cttggcgcgc cgatatggat
      181 gcatttgcca tcccggatat cttagatgtg ccgtataaat cgtcggttcc aggagtcaag
      241 cacgtgtgtg gacatgatgt tcacacaacg atcggaattg gaattgccca ggttcttgct
      301 cttctgcaag atgagatagc tggaagcata aagttcatct tccaacctgc tgaagaaacg
      361 gcaacgggcg cgcgtgctat gattcatgct ggcgcccttt ccaatcccac acccgaggtg
      421 atcttcggcc tgcacacagc tccactgccg gtagggcaaa ttggttgtgt tcccggaatg
      481 atacttcccg gaatagctaa attcgagata acactaaagt cgaagaatga gtccgtagag
      541 agacttcgtg acgtagcaga gaattgtgta gctgctcttg gctgtcttag ttccctgcgt
      601 tttccagatt cggaagaatt gtcggggttt ctgcacgcaa tggaaacagg cgagaataaa
      661 gcgcttactc aattcattct cgttagttgt tggcaagaat caggcggcat attgaagcag
      721 aatactattc aattacaggg acttgttcgt gcagccaatg agcatctaga agagcacgca
      781 aaacttatga ttcgcaagac gttagacagg atcgtaggcg aactcccggt agattacaac
      841 ttacagtggt tagaacagct ttccctgccg cctgccataa ataatgaatc actagaacga
      901 cagctaagac cggcgatcgc agccatgatt ggagcaacca atacggtcga gctgcgagct
      961 ccttttccat tcaactcgga ggatttttcg ctgtatcagc aacagcttcc cggtgttttt
     1021 tactggctgg gtgtagccaa ccttgctaaa ggaatcattg gcgtaccgca cctacctaac
     1081 ttcgatgttg atgaagaatg ccttgtggtg ggaacaaaaa caatggctca tttattgctg
     1141 cattatttcg attcctgtct tgggattcgg cactgaagaa cagttacatg cgaggatgat
     1201 tatagagtat gacttgagcg tacaggaaat ggatttgttg taaatgaatt ttgaacggaa
     1261 aaaggttcgc ttgacaaatt ggacatttca actagcaagg ggactcgaac cgctaaatac
     1321 ccatttctat tgctttcgga tctacatatc gccccaaagt attgactttc tccctagaat
     1381 caaaccttaa catagacaaa aaccgctcaa catgatctgt tggcggtctt tggcaaaccg
     1441 atgcccccaa ggggacgtgt tctgaaccac cttccccgtt tccggaggtc gatgaatctt
     1501 gcacaaaaca gcgaagatgg acaaagggcg agttcaacct gccggctata atcttgccgt
     1561 cggtgtcaac tatatttggc tgagccaaaa tctgcaagag gatagagcag tagccgcccg
     1621 tgggtctcat aatccgaagg tcgcaggctc tagtccgatt accgctaagt tacccttgcg
     1681 aaattacgtt tcgcataaag tttttgattg ttactgccat gaatcccggt gcatggggat
     1741 atacacaaca gatataattg cgttaccggt ggggtaaaca aataaccaga gtaaaaaaac
     1801 tatgtgagac tttagaaagc gatgtacgtt tgatagccca gcccaattta tgacgaatga
     1861 cacgtgaatt cctcatggat tggatatatg attcctatac aagcgtatgt tttggatgat
     1921 ttatgttggt aaaagcttct gcttgagatt gccatatgca aatttctgat ccactcatcc
     1981 ataccaaact atgcccgccc ttcatccgac cagctctggt cccgcgaccg cggctccaag
     2041 aacaaatggt gcagggattg cgcggcccgc taaccctgat tatcgccccc gctggttttg
     2101 gcaagaccac cctggtggca tcctgtctcg ctgactgcgt aacgccggtt gcctggctat
     2161 ctttggataa gagtgataat gaggtgggac aatttctgaa ttatgtaatc gcagcgttgc
     2221 aggaggctga caatacaatt ggaggtgagg ctgcccaact tatggcggga aggcagcaag
     2281 cgtcaccaga actagtccta accagtctca tcaatgactt ggattctgcc aggggtgaaa
     2341 ttaccctcgt gttggatgac taccaatttg tttgcaatca agctgtgcat gagcaactgg
     2401 cttttctgct cgagcattgc ccccccacgt tccacttggt gattgctacc cgttcggatc
     2461 ctccactccc tttggcccgc ttgcgggcgc gcggccaagt ggttgagctc agggccgtcg
     2521 atctcagctt taccgagcct gagacagctc agttcctgaa tgatatcatg ggtctgtgcc
     2581 tggatgtcag atcggtcacg gcgctcgccg agcgcaccga gggctggatt gttggattgc
     2641 aaatggccgc gctatccatg cgtgatcgta aagatgtagc tgggtttatt gaaggcttct
     2701 cggggacgaa cagatacatt ctggactatt tgctagagga ggtcttagtt agccagccgc
     2761 cagaaatcca acactttctt ctgtacacat caattttgga gcgcctgagc gcttcgctgt
     2821 gtgacgcggc cctcgcaaac aaccagccga cgaaaaggga aggcgatgat gcgtcaactg
     2881 gaggagcgct ttttctcggc agctcaacgt ccatcctcga gtatctagag agggcaaatc
     2941 tatttctgat tcccctagat gatgaaagga tctggtaccg ctaccatcat ttgtttgcgg
     3001 acttactccg tacacagctc cagaaacttc tcggtgcaca aggcgttgca caattacaca
     3061 tccgcgctgc tgactggcat gggcaaaatg gatcgctcgt ggatgctatt catcatgcat
     3121 ccatggcttc ggacgaggag agagttgagc gctttatcga acagaattat atggaattgg
     3181 tgagtcgtgg agaacagtca tggctgcgct cttggacagg taaattaagc aaagaacggg
     3241 tttatcgccg cccctggttg tgtatttatg aagcgtacag tcactcgtgg tttggagaac
     3301 tggatcaagc tgaggtgttt ttggatgcgg ccgaaaagcg cattcgatct gatatgtcgg
     3361 ttcctgacgc tcaggcaatg ctggggcatc ttacgtacat caaaagccgc gtaaccgcca
     3421 tgcgtggcga cctccctcga gccatcgagt tcaatcttgc ggctcgtgaa tatctgcaag
     3481 tcagcaacct ggctctgcag ttggatctgg gtatcacgct cggatatcta tacttcctga
     3541 atggcgatta ttctcacgcc agccaattct tgaacgagac gattcgatca ggcagagccg
     3601 tcggcgcaat cctcaacact gtggccggct attgcattat ggccaggttg cataccgtcg
     3661 agggccgatt gaatcaatca tatgagtttt atattcaagc tgcgcaatgg gtccatgagg
     3721 ttggcgggca gcatcttggc gcatctagtc tgatcgaggt tgggattgcc gatgtgttat
     3781 gtgaaaggaa tgacctggac gctgctctca ctcacatgaa gcaaggcctg gcactgcttc
     3841 cctggtgggg caagccggac gatttagccc tggcctattg caccctggtg cgccttcacc
     3901 tggcgcaagc gaatacgagt gctgcttcag aagcaatgga aaaagcgaca caggtcattc
     3961 aaacaagtgg acttttcccg gaagctcccc gtgcagtgga gcttgcccgg gtgaaactat
     4021 ggctgactca agggaatgta caggcagcgt cccactgggc cgcaacgctg gagcagcgct
     4081 ttggctcgca cgatccattc aggtttgaag aggaagtaac ttatttcgcc caggcgcgag
     4141 tatttatcgc tctgcataaa ctggatgaag ctatcagttt gctatcgcgc ctggaacaga
     4201 tcgcccgctc agcacagcgg atgggtcgcg tcatagaaat cttgctgctt gaggcgttgg
     4261 cgatgcagga gaaaggtgat ctcgagaatg ccgtgctggc gttaacaaaa tgtctgacat
     4321 tggccgaacc tgagggctat aggcgagcct ttctggatga gggccaacca gtgcagctgc
     4381 tgctcgctca gtggcttgcc cataccagcg ccagccctct gcgcgactac gctgtccgcc
     4441 tgctcgccca atttgatgct gaaccgcgtg cgattcagat ggcgcaagaa aaaacgtccc
     4501 cggctggtgg tccggtcgag ccattaagcc agcgtgagcg ggaagttctt caccttatcg
     4561 ctctgggcaa aagcaaccag gagatcgcca ggcagttgat cgtctctacg ggcaccgtca
     4621 aagcgcatac agccagtatc tatcgcaagc tcgatgttgc caaccgcacc caggcggcag
     4681 cccgcgccag gcaactcggt atccttccct attccccatt cgcaggtaaa ccctgcaaat
     4741 aaaccccaaa tatccctttt ggcagatgcg agcttcttct acgcgcgcta cattatgaat
     4801 agatactcag tagtaaggct gttctgtcgc agatcaatag caagcctggc tgaatgtcaa
     4861 ttccaaatgc tcgcgagaga gcaatctggt ctttgagacc aaaagaagct caaaatgtca
     4921 aattctggta aatggttgcg tgttcgcgta acggatctgc ggactggcgc gagcaaggca
     4981 aacgtcaaga ttccggtaag tctagccgat tttggaatga agatggcggc cagcttcgcg
     5041 ccctccgcta tcgatgattt ggacatgaac gaaatcctca cggcgatgaa aagtggcggc
     5101 gaagagaaat ttgtggagat cgaagacgaa gagaaggacg aacacatcga gatcttcatt
     5161 gaataagatc cttcgaaaac taagattaca gatcatacat gcgaatggga gcgctgacat
     5221 gccagaatac tatgagatca agatcaaggg gcatttggat gaacgttggt cggaatggtt
     5281 tgcaggcttg aagttagtct acctggaagg agatgtaacc cagttttccg gcctgctacc
     5341 tgaccaggcc gcaatatacg gtgtgctcga gcgcatgcgc gacctaaatc tgacgttgat
     5401 ttcagtcaac catggtggtc catccaccca agattgtgat aaggagaaag catgaacaaa
     5461 atcctattga gaatcggcgg ggtcatcaac ctgctgttct ttctatttca tctggctatg
     5521 gtgaaacccc tcggcgagat actggcgccg ctatccccag acatcggcgc gacggtatcg
     5581 acgttgaaca tccatgtggc atttaccttg ctgatctttg cctacctggc catctttcag
     5641 tggcatgacc tgctcacaac tcgcctgggg cagatcacag cgattgccat c
//
LOCUS       DASTJP010000013         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_103568, whole genome shotgun sequence.
ACCESSION   DASTJP010000013 DASTJP010000000
VERSION     DASTJP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103568"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VFQ23_00385"
     CDS             <1..1273
                     /locus_tag="VFQ23_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HET9905053.1"
                     /translation="SDRYAGEVARVLAANGFTVLLAQSDAPTPAISYAVKHQGAIAGV
                     MITASHNAPRYNGVKLKGAFGGSALPEQCRRVEVYITDNEERARGPNLMDFVKAREAG
                     LIQRFNPLPAYYEHVRTLINTDVIAENPQRFVVDAMYGSGRGVIKSFLQGTGCEIAEI
                     RGELNPGFGSVHPEPIGKYLGALASAVSSGMGNFGVVTDGDADRIGAMDERGTFVDPH
                     KIMALSLKYLVETRGSSGAVVRTVSTTRMIDRLAKRYGLKLYETPVGFNHIADYMMTE
                     DVLIGGEESGGISFKGHIPEGDGPIMGLLLVEMVAASGKTLQKLVEDLLADVGPAFYE
                     RTDLRLSRPVAKAEMTEFLTKQAPSDIGGEKVSEISQRDGVKYIMADDSWLLIRPSGT
                     EPVLRVYAEGRTQEMVRALLGYGEQVAETVV"
     gene            complement(1405..1620)
                     /locus_tag="VFQ23_00390"
     CDS             complement(1405..1620)
                     /locus_tag="VFQ23_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET9905054.1"
                     /translation="MNTNYKPLTRSISNRMVAGVCAGLGEYLGIDPTIIRLLTVLAFF
                     TGFGGIAIVYLIMAIVVPEQTTVQEEQ"
     gene            complement(1821..2180)
                     /locus_tag="VFQ23_00395"
     CDS             complement(1821..2180)
                     /locus_tag="VFQ23_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905055.1"
                     /translation="MNCYYHPEKPAVGLCKYCQRGLCSGCAAPAGDSLACRNLHEEQV
                     RSMETLLQKNILQSKRVGSDYMRNTIFYGTVGLLFAAFGASQIRWLGLQAVVYLVIGL
                     ALLWAAIANYLESRKYK"
     gene            complement(2183..2602)
                     /locus_tag="VFQ23_00400"
     CDS             complement(2183..2602)
                     /locus_tag="VFQ23_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET9905056.1"
                     /translation="MSVISKSISINTKGNGDIHDITNQIASAISNSGLKDGTATIFCP
                     SSTSALTTIEYESGAVSDLRRLFDEIIPVNREYAHNERWHDGNGHSHVRAALLGPSIT
                     IPFIDRQLTLGTWQQVIYVDFDNRPRQRKLIVQLIGE"
     gene            complement(2605..2934)
                     /locus_tag="VFQ23_00405"
     CDS             complement(2605..2934)
                     /locus_tag="VFQ23_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905057.1"
                     /translation="MKTLDLKKELKHLYAPSSKSPEIVQVPCLQFAMIDGVIEKGLCM
                     QMKHIKPYATEPLTINRMRAVAYENGRRDNVGPNGKHHEIYIGDPRKADPAKLKTVVR
                     HPLEKVT"
     gene            complement(2931..>3186)
                     /locus_tag="VFQ23_00410"
     CDS             complement(2931..>3186)
                     /locus_tag="VFQ23_00410"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905058.1"
                     /translation="RQTTFHHRTDQEEEIASAALDLLRSVRQPNQAVRLIGVGVSGLG
                     QPIRQLGLWDTDHEKSRKLQEAVDQLQEKYGNRVIRRGEP"
BASE COUNT          793 a          758 c          811 g          824 t
ORIGIN      
        1 atcagaccga tacgcgggag aagttgcgcg cgtcctggcg gcgaacggat tcaccgtgct
       61 gctcgcccaa tccgacgcgc cgactcccgc aatttcatac gccgtcaaac atcaaggtgc
      121 tattgccgga gtgatgatca cagcttcgca caacgcgccg cgctataacg gagtcaaatt
      181 gaagggagcg ttcggtggat cggcgttgcc ggaacaatgt cgccgcgtcg aagtctatat
      241 cacagataat gaagagcgcg cccgaggtcc gaacttgatg gatttcgtca aagcacgtga
      301 agcagggttg attcaacgat tcaatccact gccggcgtat tacgaacatg tgcggacact
      361 catcaacaca gatgtcatcg cggagaaccc gcaaagattt gtcgtagacg ccatgtatgg
      421 atcgggccgt ggcgtgatca aatcattttt acagggcaca ggttgcgaga tcgcggaaat
      481 tcgcggggaa ctgaatcccg gctttggcag cgttcatccc gaaccgattg gaaagtattt
      541 gggtgcgttg gcatccgctg tgagttcagg catggggaat ttcggtgtag taacggatgg
      601 ggatgccgac cgtatcggcg cgatggatga acgcggcaca ttcgtagacc ctcataaaat
      661 tatggcattg tcgttgaagt acctagttga aacacgcggc tcaagtgggg cggtggtgcg
      721 cacagtatcc accacgcgca tgatcgatcg actcgcaaaa cgatacggat taaagttata
      781 tgaaacacct gttggattca atcacattgc cgattacatg atgacagaag atgttttgat
      841 cggcggcgaa gagtctggcg gtatttcatt caaaggacat atccctgaag gcgatggtcc
      901 cattatggga ttgctgcttg tggaaatggt ggcagcgtca ggtaaaacgt tgcaaaagtt
      961 ggtggaagat ttgctggcag atgtgggccc tgccttctac gaacgcaccg accttcgcct
     1021 gagccgtcct gttgccaaag cagagatgac cgaattcctg acgaagcagg ctccgtcgga
     1081 tatcggcggg gaaaaggttt ctgaaatcag ccaacgtgac ggcgtgaagt acatcatggc
     1141 agatgactca tggctgctta tccgcccatc agggaccgaa cctgttctac gcgtctatgc
     1201 ggaaggacga acacaagaaa tggtcagagc tttgttgggg tatggagagc aagtggcgga
     1261 gactgtcgta taaatagatt gatgaaattt cgaactatat ctttataatg ctgttgattt
     1321 agtacaaatt gaaagcgaag agaaattaca ttttcatacg tttatttgta aaaagtccag
     1381 gctgagaagc ctggactttt ttgattactg ctcttcttgc acggtcgtct gttcggggac
     1441 gacgatcgcc atgattaaat agacgattgc tatcccgccg aagccggtga agaacgccag
     1501 tactgtcagc aggcgaataa tggttgggtc aatgccaaga tattcgccca aacctgcaca
     1561 tacacccgca accatccgat tggatatact gcgagtcaat ggtttgtaat tggtgttcat
     1621 gttgtactcc tttcaatgat ttctaaaagg acatacgcag gagagtgcat gaggttgcaa
     1681 atttagtccg gtggttgagt tgacctgagc gttctctgcg aaggtcgtat cgaaaccacc
     1741 aatttcaagc tcacctgtta tggctggttt cgataagggc aagaaaaaca tttgccctac
     1801 tcaaccagcg atgcactttg ttatttatat ttcctgctct ccaaataatt tgcgattgcc
     1861 gcccagagta acgccaggcc gatcaccaga taaaccaccg cttgcaagcc aagccatctg
     1921 atctgcgatg caccaaacgc ggcaaacaac aatcccacag ttccatagaa aatcgtgttg
     1981 cgcatgtaat ctgacccgac gcgtttcgat tgcaggatgt tcttctgcaa aagcgtttcc
     2041 atggaacgaa cttgttcttc atgcaggttc ctgcaagcca gggaatcccc ggcaggcgcg
     2101 gcacaaccag agcatagtcc gcgctgacaa tatttgcaga gacccacggc gggtttttca
     2161 ggatgatagt agcagttcaa aactattctc caatcaattg gacaattaat ttcctctggc
     2221 gcggacgatt gtcgaaatcc acatagataa cttgttgcca tgtacctaag gtcaattgcc
     2281 ggtctataaa tggaatcgtg attgacggtc caagtaaggc ggcgcgaacg tggctgtgac
     2341 cgttgccgtc atgccagcgt tcgttgtgag catactctct attcaccggg atgatttcat
     2401 caaagaggcg tcgcaaatcg ctcacagcgc cgctttcata ttcgatggtc gtcaatgcac
     2461 tggtggagga tggacagaag atcgttgcgg ttccatcttt caatccgctg ttagaaatag
     2521 cacttgcaat ttgattggta atatcgtgga tgtctccatt cccttttgta tttatggaaa
     2581 tagactttga aatgaccgac atatttatgt caccttttcg agtggatgtc tcactaccgt
     2641 tttcagtttg gcagggtcag ctttgcgcgg atcgccgata taaatctcat gatgcttgcc
     2701 attcggccca acgttgtctc gacggccatt ttcatacgcg accgctcgca ttcgattgat
     2761 cgtaagtggc tcagttgcgt agggtttaat atgtttcatc tgcatgcata accctttttc
     2821 gataactccg tcaatcattg cgaattgcag acacggaact tgtacgattt caggactctt
     2881 cgacgagggt gcataaagat gttttagctc ttttttcaag tcaagagttt tcatggttct
     2941 cctctccgaa tcaccctgtt cccatatttt tcctgcaatt gatctacggc ttcctgcaat
     3001 ttacgagatt tttcgtgatc ggtgtcccac aatcccaact gacggatcgg ctggcccaaa
     3061 ccgcttacgc caacaccgat aagtcgtact gcctgattgg gctgacgaac tgacctcagt
     3121 aggtccagcg cggcgctggc gatctcttcc tcctgatcgg tgcgatgatg gaacgtagtt
     3181 tgccgc
//
LOCUS       DASTJP010000014         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_630621, whole genome shotgun sequence.
ACCESSION   DASTJP010000014 DASTJP010000000
VERSION     DASTJP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630621"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VFQ23_00415"
     CDS             complement(<1..874)
                     /locus_tag="VFQ23_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9905059.1"
                     /translation="MAEEKKVQPLLEIRNLKTYFYTEDGVVKAVDGVNFEIYPGEVLG
                     IVGESGCGKSVTSLSIMRLIGQPGKIVEGEILYEGKDLLKLSDNEMMAMRGNRISMIF
                     QQPQTSLNPVFRVGEQISEVLNIHQDFGKEAGKKRAVELLKQVGIPDAERRAEAFPHE
                     LSGGMAQRVMIAMALACVPDLLIADEPTTALDVTIQAQILDLMRNLRSQLGAAVILIT
                     HDLGVIAEMADRVAVMYAGEVVEQTDVISLFKEPLHPYTVGLIGSTPVLGNIREKLEV
                     IPGSVPNLVRLPPGC"
     gene            complement(906..1901)
                     /locus_tag="VFQ23_00420"
     CDS             complement(906..1901)
                     /locus_tag="VFQ23_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905060.1"
                     /translation="MSAAQVASLPAEAITIEERSPFQIVMTRFRRHRLAMISLVVMVV
                     ILLITILAPVIAPFEVQELSVNKYFVPWGTVDEETGRVHYLGTDQIGRDYFSRLVYAG
                     RISLTIAIISTLISELVGIVIGAVSGFFGGWIDNLLMRFVEFMLTIPQLPLLLIISSM
                     LLRNEELIPIPEPILRFMGDLLLLTPRDARNAVLIILVLAGFSWLNAAQLMRGMVLSL
                     REQTFVEASRSLGASNYRIITRDMIPNAMAPIIVDASLGLAGFIILEAALAFLGFGIQ
                     DPIPTWGNMLAKTQEFMLDRPWLPLVPGLPIFLCSLAFNYIGDGLRDALDPRLKL"
     gene            complement(1919..>2434)
                     /locus_tag="VFQ23_00425"
     CDS             complement(1919..>2434)
                     /locus_tag="VFQ23_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905061.1"
                     /translation="EAGWPYVPAGLSESVRDYTIPLIGQVDAGSFKDRILHLILPTAV
                     LTFLNISLYSRFIRASMLEVMRQDYVRTARAKGLNERLVIMKHALRNALIPFITIVVF
                     TLPGLFSGAIITESIFAWPGMGRLYLLALGDFDYPVAMAIFFIIAILTVIATLMRDFL
                     YTLADPRIRLS"
BASE COUNT          682 a          624 c          580 g          548 t
ORIGIN      
        1 ggcatccggg aggcaaacgc accaggtttg gaaccgaccc agggatcact tcgagctttt
       61 cgcgaatatt gcccaggacc ggagttgagc caatcaggcc tactgtgtag ggatgtaatg
      121 gttctttaaa gagactaatg acatccgtct gttccacgac ttcacctgca tacatcacgg
      181 ccacacggtc tgccatttca gcgatcacac ccaggtcatg cgtgatgagg atcacagccg
      241 caccaagctg ggagcgcagg ttacgcatca gatccaggat ctgtgcctga atggtcacat
      301 ccagcgctgt ggtgggttcg tctgcaatga gaaggtcggg aacgcatgcc agcgccatgg
      361 caatcatgac acgttgtgcc atgccgcctg aaagttcatg aggaaatgct tctgcgcggc
      421 gttcagcatc cgggataccc acttgcttga gcaattcaac ggcgcgcttt ttgccggctt
      481 ccttaccaaa atcctggtga atattcaaaa cttcactgat ctgttcgccg acgcgaaata
      541 cggggttcaa ggaagtctgt ggctgctgga agatcattga aatgcggttg ccccgcatcg
      601 ccatcatttc gttatcagag agttttagca gatccttccc ctcatataaa atctcacctt
      661 cgacgatttt tccgggctgg ccgatcaatc gcatgataga aagtgatgtg acactttttc
      721 cgcagccgga ttcaccgaca atccctaata cctcaccagg gtaaatttcg aaattcaccc
      781 catccactgc ttttacaaca ccgtcttctg tatagaaata tgtttttaga ttacgtatct
      841 caagcaaagg ttggactttt ttttcttcag ccacgattca tctccaatgt aaaatcccca
      901 ccttcttata gtttcaaacg cgggtcaagc gcgtcgcgta aaccgtcacc aatataattg
      961 aatgccagag aacaaaggaa aattggcaaa cccggcacca gaggtaacca ggggcgatcc
     1021 aacataaatt cctgcgtttt cgcgagcata ttgccccagg ttgggattgg atcttgaatc
     1081 ccaaacccta gaaaagccag ggcagcttcc agaataataa agcctgccag ccccaatgat
     1141 gcatcgacta tgatcggcgc catggcattg gggatcatat ctctggtgat aatacgataa
     1201 ttggatgcgc caagtgaacg agaggcttct acaaaggtct gctcacgcag agaaagcacc
     1261 atgccgcgca taagttgtgc ggcgttcagc caactgaagc cagctaacac gaggatgata
     1321 agaaccgcat tccgtgcatc tctgggcgtc aacagcagga gatcgcccat gaaccttagg
     1381 attggctctg gaatgggaat aagttcctcg tttcttaaca gcatggatga aatgatcaac
     1441 aacaatggca attgcggaat ggtcaacata aattccacaa agcgcatcag gaggttatca
     1501 atccaacccc caaagaagcc ggagactgcg ccaattacaa tgccgaccag ctcagaaatc
     1561 aacgtggata tgattgcaat ggtgagagaa atgcgcccgg catacaccag gcgtgaaaag
     1621 taatccctac cgatctggtc ggtgcccaga taatgaacgc gcccggtctc ctcatccaca
     1681 gtcccccatg gaacaaaata tttattaaca gacaattcct gaacttcgaa tggagcaatc
     1741 acgggcgcga ggatggtaat aagtaatatt acgaccatga cgaccagcga tatcatcgcc
     1801 aggcgatgac gcctgaagcg tgtcatcaca atctggaaag gtgagcgttc ctcgatggtt
     1861 attgcttcgg cagggagact ggctacttgg gcagcggaca taatgttatt accttattct
     1921 aggatagacg gattcgagga tctgcgagcg tgtaaagaaa atccctcatc aaggtcgcta
     1981 ttacagtcaa gatcgcaata atgaaaaaga tcgccattgc aaccggataa tcgaaatcac
     2041 ccagcgccag gaggtacagg cgtcccattc cgggccatgc aaaaatactc tccgtaatga
     2101 tcgccccgct gaacaagcca ggcaacgtaa aaaccacaat ggtaataaat ggaatcagtg
     2161 cattgcgcaa agcatgcttc atgatcacga ggcgttcatt cagacctttg gcgcgggcgg
     2221 tccgcacgta atcctgtcgc attacctcaa gcatgcttgc ccggatgaaa cggctgtaca
     2281 acgaaatatt taaaaaggta agaacagccg ttggcaggat caagtgtaat attcggtcct
     2341 tgaagctgcc tgcatccacc tgtccaatga gagggatggt gtaatcacgg actgattcag
     2401 acaaaccagc cggcacatac ggccaaccag cttc
//
LOCUS       DASTJP010000015         3182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1053426, whole genome shotgun sequence.
ACCESSION   DASTJP010000015 DASTJP010000000
VERSION     DASTJP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053426"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..986
                     /locus_tag="VFQ23_00430"
     CDS             168..986
                     /locus_tag="VFQ23_00430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9905062.1"
                     /translation="MSTQEIVSSPNLKELSTTYEKGQYTLWQILGIWALAALPMGLIM
                     WVVVPLLIPHVDMEPGFLFFILMALGLVWQGILAYIILRREVKPFTWENIKARLWLYT
                     PADPRTGIRSNRSYLWTIPLIVFSQGYYALDVLGGLDELWVKTFPFLAPRPYAVIQNV
                     AEPIVGQWWMLGVLAIMLVFNYLLGEELIFRGILLPKMNGVFGKWDWIANGILFSTYH
                     LHRIWGLPSTLFIDWIYPWAAKRFHSYWVAVILHAVDGVILVVLFPMAIMGLLS"
     gene            complement(1256..2035)
                     /locus_tag="VFQ23_00435"
     CDS             complement(1256..2035)
                     /locus_tag="VFQ23_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9905063.1"
                     /translation="MSTPKGYVGSEYLHLLEEQMMDYKRRSYARMNIQPGDSVLDVGC
                     GPGSDTIHLAQLVGAAGQVVGVDYDEDMVAQANQRAHAAGLAEWVKHQQGNATSLPFP
                     DAQFHACRSERLFQHLLFPEQALFEMARVTKPGGWVVVLDTDWGTLSIHTSLPEIERR
                     LVQFSNEGCMHNSYSGRRLPQLFAAQGLRNISVEVLPFPITNYALCREVIVLDRREQE
                     ALAADAIRQDELDRWHEDLEALDRNGGFFASFNLVLVAGIV"
     gene            complement(2047..2841)
                     /locus_tag="VFQ23_00440"
     CDS             complement(2047..2841)
                     /locus_tag="VFQ23_00440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905064.1"
                     /translation="MNITHQDVLRSPQLFEDGTFEYLGFITSLPKFQRRVETDVTMIT
                     ADAPVFYFNAAYNTRFNGNAKQRIKETKEFFLENGRSFVWQVTPSSQPENLGEQLIQQ
                     GGKLLESMPYMALQLDHIWRDSIPTVFHCEAVRTHEMLYLWISVYCRARAYPGDTDRL
                     FNILSDLDLSEASPLQLILGYLGGAPVATYSVLMGSEVAGFYSLTTLPEARGQGLGTA
                     ISTAAADLALDRGYKTAILLSEPMSRNLCKRLGFVEARCHMDIYRF"
     gene            complement(2861..3004)
                     /locus_tag="VFQ23_00445"
     CDS             complement(2861..3004)
                     /locus_tag="VFQ23_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905065.1"
                     /translation="MFNTILLITVFVIVQVICIAVAWKQAPDEQDLSDEERQAALWRF
                     YHP"
BASE COUNT          776 a          793 c          818 g          795 t
ORIGIN      
        1 ttctcagggc tgatcaacga tggtgatctt tacaacgctg cctatcactt atcagacgtg
       61 acgatgcagt tcgatatggc agccgtattg accagatcaa cctggaagta gaagacaaag
      121 cgacgtcatc cgtctcttgc aagcaaatca taaaaaggag aaatatcatg tcaacgcaag
      181 aaattgtctc atcgcctaac ttgaaggagc tttcaacgac ctatgagaaa ggtcaataca
      241 ccctctggca aatcctcggg atttgggcgc tggccgccct gcccatggga ctcatcatgt
      301 gggtggttgt gccgcttctg atcccgcatg tagatatgga gccaggattc ctatttttca
      361 ttttgatggc gttagggttg gtctggcagg gcatccttgc ttacattatt ctcagacggg
      421 aagtgaagcc gtttacctgg gaaaacatca aagccagact gtggttatac acccccgcag
      481 atcccaggac gggcattcga tcgaatcgat catacctctg gaccataccg ttgatcgtgt
      541 ttagccaggg atattatgcg ctggatgtcc ttggagggct ggatgaactt tgggtaaaga
      601 ccttcccgtt tttggcgccg cgcccctatg cggtgatcca gaacgtggct gaacccatcg
      661 tgggacaatg gtggatgctg ggggtcctgg ccatcatgct ggtattcaac tacctgctgg
      721 gagaagaatt gatcttccgg gggatactct tgccgaaaat gaatggagtg ttcggcaaat
      781 gggattggat tgccaacggt attctgttct caacatacca tttgcatcgg atctggggac
      841 tgccgtcaac gctctttatt gattggatct atccctgggc ggcgaaacga ttccattcgt
      901 attgggtggc cgtcatcctg cacgctgttg acggtgtaat tctggtcgtc ctcttcccga
      961 tggcaattat gggtttactc agttagtagg gatttagaga tctgccgtgc cagttgagaa
     1021 cgccgaccag catctgttgg tcggcgtttg ctctgagtgt aataggtcag gtgatcgagt
     1081 tgacctggtt caatatatgg gttttggaca acgtttgtca acaatggggc ggttcgtcgc
     1141 tcgtcgcagg aacctatgcc acacctggca acgaatcact ccctcaaatt agctgcatcc
     1201 taaatcattt tcctttcatt tcagacaaaa taactggact atttatgcga acggttcaaa
     1261 caattccagc gacaagcacg agattgaacg aggcaaagaa acctccatta cggtcgagtg
     1321 cttccaaatc ttcatgccag cgatctaatt catcctgcct gatagcatca gcagcaagcg
     1381 cctcttgttc acgccgatcc aacacaatga cctcacgaca aagagcatag ttcgtgatcg
     1441 gaaatggaag gacttccacc gaaatattcc tgagaccctg tgctgcaaac agttgaggca
     1501 gtcgacggcc cgaatagctg ttatgcatac aaccctcatt agagaattgg acaaggcggc
     1561 gttcgatctc cggcagtgag gtgtggatgg aaagtgtgcc ccaatcggtg tccagaacga
     1621 ctacccagcc accaggcttt gttacgcgtg ccatttcaaa tagtgcctgc tcgggaaaca
     1681 agagatgttg gaagagtctt tcactccggc aagcatggaa ttgagcatca ggaaacggaa
     1741 gggaggttgc gttgccttgc tggtgtttca cccattcggc gagaccggcc gcatgcgcgc
     1801 gttgattcgc ctgcgcgacc atatcttcgt cgtaatccac gccgactact tgacccgccg
     1861 cgcccaccaa ttgagccagg tgaattgtgt cggagccggg gccgcagcca acgtccaata
     1921 ctgaatcccc aggctggatg ttcatgcgcg cgtaactcct gcgcttgtaa tccatcattt
     1981 gctcttcgag tagatgtaga tattccgatc cgacgtatcc ttttggtgtt gacattacat
     2041 tctccttcag aatcgataaa tatccatatg gcaacgggcc tccacgaagc caagccgctt
     2101 acatagattg cgcgacattg gttcggaaag gagtatcgca gttttgtagc cgcgatccaa
     2161 cgcaaggtct gcagcggcag tggagatagc tgtaccgagt ccctgaccgc gcgcctcggg
     2221 cagagtcgtt agactataga atcccgcgac ttcgctcccc atcaaaaccg aatacgtagc
     2281 aacaggcgca ccacccagat acccaagaat gagttggagg ggcgacgctt cgctcaagtc
     2341 caaatcggaa agaatattga atagcctatc agtgtccccg ggataggctc gcgcgcggca
     2401 atagaccgag atccacagat ataacatttc atgagtccgg accgcttcac agtgaaagac
     2461 tgtggggatt gaatccctcc aaatgtgatc caattgaaga gccatgtaag gcatggactc
     2521 caaaagtttc ccgccttgct gtatgagttg ctcgcccagg ttttcaggtt gggacgaagg
     2581 cgtcacttgc catacgaatg accggccgtt ttcaaggaag aattccttcg tctccttgat
     2641 cctttgcttt gcgtttccat tgaaacgggt attgtaggcg gcgttgaaat agaacacggg
     2701 cgcgtcggct gtaatcatcg ttacatctgt ttcgacgcgc ctctgaaatt tcgggagaga
     2761 ggtaataaac cccagatact caaatgtgcc atcttcaaat agctgcggcg atctcaacac
     2821 gtcttgatgg gtgatattca ttgcaatttc cctttcgaaa ttacgggtga taaaatctcc
     2881 atagagcggc ttggcgctcc tcgtcactga ggtcttgttc gtcgggggcc tgcttccagg
     2941 caacggcaat gcagatgacc tggacaatca caaaaaccgt gataagaagg atagtgttaa
     3001 acatggtggt ctcctgaatg gatgatggtg atgtgctcaa tctatgatct cagtgtacag
     3061 gcgcaccgtt ccccaccatg ttcgcgagcg tagattgagc gtattttttc ggcgttttcc
     3121 cgaaacgtcg caaattaaag cgaaaaatcc caccgctcct ttcaggagga gcagtgggat
     3181 ga
//
LOCUS       DASTJP010000016         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1053289, whole genome shotgun sequence.
ACCESSION   DASTJP010000016 DASTJP010000000
VERSION     DASTJP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053289"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFQ23_00450"
     CDS             complement(<1..648)
                     /locus_tag="VFQ23_00450"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HET9905066.1"
                     /translation="MGTTSPANPSLSTTPSATVAQPIPTEPVSEPTQVIEVINPLTGL
                     PIANPSLLEVPAALVSISHFPSTARPQAGLSFAPYVFEFYITEGATRFLSTFYGDMPM
                     PEPHITGDCEIRNEPFQQTGEILGNQVWLDSNADGLHEVWERGVGGICVNLYDASLSL
                     LQQTTTDSNGYYGFNVEPGTYFVEFLKPSGMEFTRRDAKADEQDSDVDQATGRSEL"
     gene            complement(769..1257)
                     /locus_tag="VFQ23_00455"
     CDS             complement(769..1257)
                     /locus_tag="VFQ23_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET9905067.1"
                     /translation="MNHIWSPWRMEYIENNNKPDGCVFCIAQAMQDSPENLIAHRGQH
                     AYVILNRYPYTSGHLMVVPFAHKANLETLDSQTRAEMMELTTRSMTVLRKVYNPQAFN
                     MGANIGEAAGAGVKEHVHIHIVPRWKGDTNFMSTLGDTRVLPETLEDTYQRVKQGFQA
                     IR"
     gene            1362..>2035
                     /gene="xseA"
                     /locus_tag="VFQ23_00460"
     CDS             1362..>2035
                     /gene="xseA"
                     /locus_tag="VFQ23_00460"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET9905068.1"
                     /translation="MQSNLFPAQSAQQWTVSRLTFYIRKLLEEDEILQDVWVQGEISN
                     LSKPASGHVYFTLKDSSAALKCVMWKTSAMRLSLPLQNGMAVEVHGKIGIYEVSGQYQ
                     LYADQMRAVGEGALYQEFLRIKSMLEAEGLFAQERKRDIPVFPRKIGIVTSATGAALR
                     DMLNTLRRRLPLVEVILAPSPVQGVEAPPALIKAIRALNDRSPDVIILARGGGSIEDL
                     WAFNDER"
BASE COUNT          478 a          508 c          535 g          514 t
ORIGIN      
        1 cagctcgctc cgcccggttg cctgatccac atcactatcc tgttcatccg ctttggcatc
       61 tctccgggta aattccatcc cagagggctt caaaaactca acaaagtagg ttcccggttc
      121 tacattgaat ccataatacc cattgctatc ggtggtcgtt tgttgcagca gcgagagact
      181 cgcgtcataa aggttgacac aaatacctcc aacgccgcgc tcccacactt catgcagtcc
      241 atcggcattt gaatccaacc agacctggtt tccaagaatc tcgccggtct gctggaatgg
      301 ctcatttcgg atttcgcagt caccagttat gtgtggttcg ggcattggca tgtcgccgta
      361 aaacgtcgag aggaagcgag ttgcaccttc tgtaatataa aactcgaaca catagggtgc
      421 gaatgacaac cctgcctgcg gccgcgccgt cgaaggaaag tgagagatcg aaaccagcgc
      481 tgccggaact tcgagcagtg acggatttgc aattggtaga cctgtcagcg ggttgatcac
      541 ttcgatgact tgtgttggct cgctgaccgg ttctgttggt atgggttgcg cgactgttgc
      601 tgatggtgtt gtggagagcg atggatttgc aggtgaagtg gtacccactg gaaggctggg
      661 actgcaattg ttaagtgcaa tagttattaa gatgattgag agcagaggga aacgattact
      721 ggaacgatgc atggcggata aagatgacag tcactttcaa tgtgactgtc atctgatagc
      781 ttgaaagccc tgtttcacgc gctgatacgt atcttccagt gtctctggta gcacacgagt
      841 atcccccagc gtggacataa aattcgtatc gcctttccag cgagggacga tgtgaatatg
      901 cacatgttcc tttaccccgg caccggccgc ttctccaatg tttgccccca tgttgaatgc
      961 ttgcggatta tagactttgc gcaggacagt catggaacgc gtggtcaatt ccatcatttc
     1021 ggcgcgggtt tgagagtcga gcgtttcgag attcgcttta tgtgcaaacg gaacgaccat
     1081 caaatgcccg ctggtgtaag gataacggtt gaggatcaca taggcgtgtt gaccacggtg
     1141 cgcgatcagg ttctcgggac tgtcttgcat ggcttgcgcg atgcagaaca cacatccgtc
     1201 aggtttgttg ttgttttcaa tatattccat acgccacggt gaccaaatgt gattcataga
     1261 aggggtgcat aacgaatttt aacagagaaa tcagccagac aacttgccaa tttgccctat
     1321 tctgttgaca ttcttctaca ccttgggtta tcctccctgc aatgcaatcc aacctttttc
     1381 ccgcgcaaag cgctcaacaa tggactgttt cacgactcac gttctacatc cgcaagctac
     1441 ttgaagagga tgagatcctt caggatgtat gggtgcaagg ggagatctcg aatctgtcaa
     1501 aacctgcatc tggtcatgtg tatttcacgt tgaaagactc ctctgcggcg cttaagtgcg
     1561 taatgtggaa gaccagcgcc atgcgcctca gcctgccgct tcaaaatggc atggcagtgg
     1621 aagtgcatgg caagatcgga atctatgagg taagcgggca atatcaactc tacgccgatc
     1681 agatgcgcgc cgttggcgaa ggcgcgttat atcaggaatt cctgcggatc aagtcgatgc
     1741 tcgaagcgga gggtcttttc gctcaggaac gcaaaaggga tattccagta ttcccgcgca
     1801 aaattgggat tgtcacctct gccacaggcg cagcgttgcg cgatatgctc aatacgctga
     1861 gacgtcgtct tccgcttgtc gaagtgatcc ttgcgccctc acccgttcaa ggagttgagg
     1921 cgccgcctgc cctgatcaaa gccatccgag cactcaacga ccggtcgcct gatgtcatta
     1981 ttctggcacg cggcggaggt tcgatcgaag atttgtgggc cttcaatgat gaacg
//
LOCUS       DASTJP010000017        10692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_841218, whole genome shotgun sequence.
ACCESSION   DASTJP010000017 DASTJP010000000
VERSION     DASTJP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841218"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFQ23_00465"
     CDS             <1..594
                     /locus_tag="VFQ23_00465"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9905069.1"
                     /translation="VNSSRKWEADYARHTDGWDLGGPTPVFEQLLSTRHLPPGRMIVL
                     GAGRGHDARLFARHGFQVTAVDFASQAVQEMHLLADPTAPVEILQHDIFTLPEALNGS
                     FDYVLEYTCFCAIDPKRRAEYADLVTRLLKPNGMYIDLAFPLDGRKGGPPFAVTEAEI
                     FDLFEQRGFKLLSREKPVESVSPRRHAEELFIFQKTY"
     gene            741..1730
                     /locus_tag="VFQ23_00470"
     CDS             741..1730
                     /locus_tag="VFQ23_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET9905070.1"
                     /translation="MKRFTTFALLLMVSIVLAACGPAQTAVPTTMPEPTAMPEPTASP
                     EPVLKDIVDTAVDDGRFTTLVAAAQAAELVDTLKGEGPYTVFAPTDDAFAALPAGTLD
                     ELLMPENKQNLSDILLYHVVPGRVMSTDVTALNSATTVFGEDVAIKSDMGDVYVNDAK
                     IILTDIETSNGVIHVIDAVILPPAEQPAAEANGTIVDVAVADGRFTTLVAAVQAAGLA
                     ETLSGEGPFTVFAPTDDAFAALPAGTVESLLLPENKQQLIDILLYHVVSGKVMAEDVI
                     SLTSATTELSTDITIKVEDGKVFLNDTVEVIITDIETSNGVIHVIDAVLLPPQ"
     gene            complement(2037..2789)
                     /locus_tag="VFQ23_00475"
     CDS             complement(2037..2789)
                     /locus_tag="VFQ23_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9905071.1"
                     /translation="MFKPQSVLWFVVLTVALSFATYFLPLPVETKSLLVPLMLAFLPA
                     LVAVLIAYFTEGGEGIRQLFSTVRGAGRWVLIGALLGAFMRVAVLVIGKLMGMSIQAD
                     FSAPGTIFIILITIPFAWFEEFGWRRFALDRLLRSRSPIAASLLLGIPWAVIHIILLF
                     PGMMSVGAPALPQTIVLVAMSIVLTWAYVRSGGSLLTVTLVHGVQNALVILNRGVSVA
                     EGTWLLMGVYVLIAATLIIVDRKIFNTQPSSA"
     gene            complement(2955..3971)
                     /locus_tag="VFQ23_00480"
     CDS             complement(2955..3971)
                     /locus_tag="VFQ23_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905072.1"
                     /translation="MKHWLNLVGSAVMLWTVTACANASTQVPTPAITQQMPAASPVPS
                     GSELYGVFQANTPCSAEARPLPQIPADAACEQMKWNLILYQDTETGAPTTYNLTSSYG
                     MAKPNTNDLIDGGTSIEMKGNWTITAGTKSNPEAIVYQLNDKDSQDSVSFLKVSDDML
                     HVLGREKALLVGNGAWSYTLNRMDHKSPTEIVEQSRPDPPPRPPQPAMPAGSSIFAIF
                     DGRTPCDEVVLEFTNTESFPGCLKIKWRLTLYQDSASGEPTRYLFMSTSQFREGSWRI
                     ISGTDVDPNTVIYELQPVDTKESFYFQKVDKNHLYLMDGEQNLLIGNELFSYTLSRVE
                     PGVQ"
     gene            complement(4040..4735)
                     /locus_tag="VFQ23_00485"
     CDS             complement(4040..4735)
                     /locus_tag="VFQ23_00485"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET9905073.1"
                     /translation="MSKHNPSFVDWRAVARTHPSAFLLAAQLLSLFLYAALDGNSSGR
                     VILGAFGILILTLVVWVITRSPALNWIAWVLVAPAILLSLLWILIPNPSLLMWSSLLE
                     AVLYFYAAGSLVAYMMKDYRVTSDELFAAGATFTLLAWGFAYVYLACQMWSPRSFVGL
                     IDPEQPRTFVELLSLSFTNLTATGLGDVLPVAPLARVLVMLEQFSGIGYVAVVVSRLI
                     GLTLVQQKGKKTS"
     gene            complement(4740..4934)
                     /locus_tag="VFQ23_00490"
     CDS             complement(4740..4934)
                     /locus_tag="VFQ23_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905074.1"
                     /translation="MEIFTSLVAGMLAGGSYGAVTGRAIEQIIRLPSAGHLSKSNKIK
                     IECTARALIVRFVGGIQGYN"
     gene            complement(4961..5206)
                     /locus_tag="VFQ23_00495"
     CDS             complement(4961..5206)
                     /locus_tag="VFQ23_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905075.1"
                     /translation="MLRSRNRLAHALWLPASTISGLVGYLLIASLGVRVLSPVLIAYT
                     APYENFAIHLIFHTFLWTVIGLGQWPLLRGVVPRAGR"
     gene            complement(5374..5613)
                     /locus_tag="VFQ23_00500"
     CDS             complement(5374..5613)
                     /locus_tag="VFQ23_00500"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905076.1"
                     /translation="MSKGLKFSSHSALIASYGKEFAKTGLLDPKFHRYIVVAQKRRET
                     GHYDAEPVITEEQAIESFQWANEFREAVTNYLSKI"
     gene            complement(5598..5756)
                     /locus_tag="VFQ23_00505"
     CDS             complement(5598..5756)
                     /locus_tag="VFQ23_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905077.1"
                     /translation="MSKEEIQLLLDKADDSHAAAKLLIDRGFIGFSAAQSYYTIFTWL
                     RPCYCQKA"
     gene            complement(5753..6076)
                     /locus_tag="VFQ23_00510"
     CDS             complement(5753..6076)
                     /locus_tag="VFQ23_00510"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9905078.1"
                     /translation="MPRNIKKILQELKKGLVEIYGDQLKTVVLFGSYARGDAHPPDSD
                     IDVMIVLNGDVDYVDAEKRSSEFISSLCLKHDVLISWVFASNKNYMENQMPFMLNVRA
                     EGIAV"
     gene            complement(6297..7157)
                     /locus_tag="VFQ23_00515"
     CDS             complement(6297..7157)
                     /locus_tag="VFQ23_00515"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9905079.1"
                     /translation="MKKVLLASVLAMMLVLSLAGGAGAITNGQPDGNAHPYIGLLVFD
                     ADIPGVGVVPAWTCSGALLSPTVVLTAGHCTDGAVAARIWFNEKLQNPDFSFIVSDFP
                     FGGGESTEGTPHTNPDYGNSPLNSNGLPGGAYRDVGIVVLDEPVDVGGFAALPAAGLV
                     DTLKNKTKVDFVGYGVQFQAQIPGSLLPQPPPFYRWDGYYNRLYAPSELVSGNFAHSA
                     EFVRLALNPGGGSGGLCFGDSGGPDLLRGTNVVLGVNSYVTNINCSGVGYSSRVDIPD
                     VLAWINSFLN"
     gene            complement(7404..8345)
                     /locus_tag="VFQ23_00520"
     CDS             complement(7404..8345)
                     /locus_tag="VFQ23_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905080.1"
                     /translation="MRTNLILTIAVMLMMVSCNTGQVPDKSYKPQVSDPAYGTGEGLV
                     VCLDEAHNNFHTLKNRFWAFGELLRRDGYVVESSSEKFSAAVLERCDIMVIANAQLEL
                     DGWGEYPYPTPSGFTDEEIVAVGDWVNGGGSLLLIADHMPLAGVAAKLASAFGVEFND
                     GFAVAGFSDEKSRDAAFGIPTIFSVDANTLIPNAITQGRNETESVTSIRSFTGQAFQA
                     PESAQPLMVLPNDFVLLMPEEAWVFTPDTKTLPVGGWLQGAVMPFGSGRAAFFGEAAM
                     FTAQLTDNQPSGMNAPMAEQNFQFVLNVMHWLSGILN"
     gene            complement(8525..8680)
                     /locus_tag="VFQ23_00525"
     CDS             complement(8525..8680)
                     /locus_tag="VFQ23_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905081.1"
                     /translation="MRHLSSNQGPLWETKEDADAMNASLAQTLALFDGLFAGQPSLDT
                     YEELLQV"
     gene            complement(8680..9078)
                     /locus_tag="VFQ23_00530"
     CDS             complement(8680..9078)
                     /locus_tag="VFQ23_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905082.1"
                     /translation="MNTKLLMTASSLVLGLTGIVALFAPDVLLAMLGVPMANPLSVLI
                     QLLGALYFSIAMMNWTAKDSAIGGIYARPVSLANFAHFFIGALLLIRYQLSNEFNLLI
                     FLVLVVYVIFAALFYWLVFRATGIPVNKNA"
     gene            complement(9309..9749)
                     /locus_tag="VFQ23_00535"
     CDS             complement(9309..9749)
                     /locus_tag="VFQ23_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9905083.1"
                     /translation="MSSETNKTVSRRFFDEVFSKGNLAVADQIIVKDQVNSGPGNPSG
                     LLPGLEGTKQLVTMYRNAFPDVHFTIDEQIAEGDKVMTRWTAHGTHQGELAGIPATGK
                     SSTVTGIAIDRLVNGKIAESWGIFDEFGMMQQLGVIPVPELAGR"
     gene            complement(10117..10404)
                     /locus_tag="VFQ23_00540"
     CDS             complement(10117..10404)
                     /locus_tag="VFQ23_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9905084.1"
                     /translation="MYARMINSQLVAGKTEEAVSIWRDKVAPGLKDVKGFKGAYLVGD
                     RGTGKGVTITLWETKEDADAMNASLQQTLALFDGLFVGQPSLDTYEVLVQV"
BASE COUNT         2727 a         2828 c         2597 g         2540 t
ORIGIN      
        1 gtcaattcgt ctcgcaaatg ggaagcggat tacgcgcgtc acacagatgg ctgggacctg
       61 ggcggaccga cacctgtctt tgagcagctc ctctcaaccc gacacctgcc tccggggcgg
      121 atgatcgttc tcggcgccgg acgtggtcac gacgcgcgct tgttcgcgcg gcatggcttt
      181 caagtgaccg cggtagactt tgcgtctcag gcggttcagg agatgcatct ccttgctgat
      241 ccgactgctc ctgtagagat tttacagcat gatattttta cgctgccaga agcgttaaac
      301 ggcagctttg attacgttct cgaatatacg tgtttttgcg cgattgaccc caaacgccgc
      361 gcggaatacg cagatttagt cacacgtttg ctgaaaccca atggtatgta catcgactta
      421 gctttcccgc tggatggacg aaagggtggc ccgccgttcg cggtgaccga agccgagatc
      481 ttcgatttgt ttgagcagcg cggattcaaa ttactgtcgc gtgaaaagcc cgttgaatcg
      541 gtttcgccgc gcaggcatgc cgaagagttg tttatttttc aaaagacgta ttgaaacaca
      601 gccagtaaat gggggagaaa ctgagcgttt cattagaagc acatctctcc cattgacaac
      661 ctttgcaaaa catgtacaat ataggcaata cctgtgcaaa atatatacac actgtacagg
      721 attcaaaaag gagaaaagaa ttgaaacgct ttactacgtt cgcactattg ctaatggtat
      781 ccatagtttt ggcagcctgt ggtcctgcac aaactgcagt ccccaccaca atgcctgagc
      841 cgacggcaat gccagaaccc actgcatcac cagagcctgt cctcaaggac attgttgata
      901 cggctgttga tgatggccgc ttcacgacac tggtcgctgc agcccaggcc gctgaactgg
      961 tggacaccct caaaggcgaa ggtccttata ccgtattcgc ccccactgat gatgcctttg
     1021 ctgctctgcc agccggtacg cttgacgaat tgctaatgcc ggaaaacaag cagaacctgt
     1081 ccgatatttt gctctaccat gttgttccgg gcagagtgat gtccacagac gtgaccgctc
     1141 tgaacagtgc caccactgtg tttggtgaag atgttgccat caagtctgat atgggcgatg
     1201 tgtatgtcaa tgacgcgaaa atcatcctca ccgacatcga aacctcaaat ggtgtgatcc
     1261 atgtgatcga tgcggtcatc ctgcccccgg cagagcaacc agccgctgaa gcaaacggga
     1321 ccattgttga cgtcgctgtg gctgacggtc gctttaccac tctcgttgct gcagtccagg
     1381 ctgcgggttt ggctgagacg ttgagcggcg aaggtccctt cactgtcttt gcacccacag
     1441 acgatgcttt cgctgcttta ccagccggga ccgttgaaag cctgctcctc ccggagaaca
     1501 aacagcagct cattgacatc ctgctctatc atgtggtctc gggcaaggtg atggctgagg
     1561 acgtgatctc tctgaccagc gccaccaccg agcttagcac ggatatcacc atcaaggtgg
     1621 aggatggcaa ggtcttcctg aatgacacag tcgaagtgat cattacagat atcgaaactt
     1681 ccaacggcgt gatccacgtg attgacgcgg tgctcctccc gcctcaataa acgtatctgt
     1741 cccctcagaa gaaagtgtaa aacaaaagga cgggcttagc tgagcctgtc cttttgttgt
     1801 tttatttgat ttctctctta agtacctgga tcaactctgt ttaatgtata gctaaaaagc
     1861 tcattgccga tcagacggtt ttgctcacta tacatgaggt agagatggtt ctcgtccact
     1921 ttcaggaagt agaaagattc tttcgtatca tccggctgga gttcgtatat aacagtattc
     1981 ggatcaccac ctataccctt gatgatcctc caccgtttga cgatagtgat cgactcttag
     2041 gctgaagagg gctgggtatt aaatatctta cgatcaacga tgatcaatgt tgcagcaata
     2101 agaacataga ctcccatcag caaccacgtt ccttctgcga cgctcacacc gcggttaagg
     2161 atcaccaatg cgttttgaac cccgtgcacc agggtaaccg tcaacagact gccgccactg
     2221 cgaacatatg cccaggtgag gacaatactc atggcaacta atacgatcgt ttgggggaga
     2281 gccggcgcac cgacggacat catccctggg aagagcagga taatgtggat caccgcccag
     2341 ggaattccca gcagaagaga agctgcgatc ggggaacgcg accttagcaa acggtccagc
     2401 gcgaaccgcc gccagccgaa ctcctcgaac caagcaaatg gaattgtgat aaggataatg
     2461 aaaatggttc ccggcgcact gaagtctgct tgaatagaca tacccataag ctttccaatt
     2521 acgagcacag cgactcgcat gaatgcaccc agcaatgccc cgatgagtac ccacctcccg
     2581 gcaccgcgga cagttgaaaa tagctgacga attccctctc cgccttctgt gaagtatgca
     2641 atcaaaacag ccaccaaagc aggaaggaaa gccagcataa ggggaaccag cagagatttc
     2701 gtctctactg gcagagggag aaaatacgtt gcaaatgaaa gggcaacggt gaggacgaca
     2761 aaccaaagaa cggattgagg tttgaacata gcgaggttct tctttcatcc ggcctacatt
     2821 gtgagtagtg ccgttcatgt gaacaaaccc cactgcctcc gaagaagttc ctctctgtat
     2881 cagatcctaa aggtctttgc gaaataccct cgaaacgtaa tggaaaagcc gaagaccttt
     2941 aggatctctc taaattattg aacgcctggc tccaccctgc tcaatgtata actaaaaagc
     3001 tcattcccaa tcagaaggtt ttgctcgcca tccatcagat agagatgatt cttatccacc
     3061 ttctgaaagt agaaggattc tttcgtatcg accggctgga gttcgtatat aacagtattc
     3121 ggatcaacat ctgtgccact gatgatcctc cacgagcctt ctcggaattg gctcgtgctc
     3181 atgaacagat atcgagtcgg ctcgcccgac gcgctgtcct ggtataacgt gagccgccat
     3241 ttgattttca ggcaacccgg aaacgattca gtatttgtaa attcaagcac aacctcatca
     3301 cagggagtgc gtccatcgaa tatagcaaag atggatgacc ctgcaggcat cgcgggttga
     3361 ggcggacggg gcggagggtc tggacgtgat tgttcgacga tctcagttgg gcttttatga
     3421 tccatgcgat tcagcgtata actccaggcg ccattaccga ccagaagagc tttttcgcga
     3481 ccgagaacat gaagcatgtc atcgcttact tttagaaatg acacgctgtc ttgagagtcc
     3541 ttatcgttca gttgataaac aattgcttcc gggttgctct tggtcccagc ggttatcgtc
     3601 cagttccctt tcatctcgat cgatgtgcca ccgtcgatca ggtcattggt gttgggtttc
     3661 gccatgccat aagaacttgt caaattatag gtagtgggag cgccggtctc tgtatcctga
     3721 taaaggatca gattccattt catttgctca catgccgcat ccgccgggat ctggggtaag
     3781 ggccgtgcct ccgcgctgca aggcgtatta gcctggaaca cgccataaag ctccgatccg
     3841 gacggcaccg gtgatgctgc gggcatttgc tgggtaatgg caggagtagg tacttgcgtg
     3901 ctggcgtttg cacaggctgt aacggtccat aacattacag ccgacccaac taaatttaac
     3961 cagtgtttca tgggtttatc cttttataaa aataccccgc tgctctgcta ttcgttccct
     4021 ccagggaaga gacccggcct caggatgtct tcttcccttt ttgctgcacg agcgtcagtc
     4081 cgatcaggcg cgagaccacc accgcgacat agccgatgcc ggagaactgc tccagcatca
     4141 cgagcacgcg cgccagtgga gcaaccggca ggacgtcgcc caggccggtt gccgtcaggt
     4201 tggtgaagct cagggacagg agttcgacga aggtcctcgg ttgctccgga tcgatcaggc
     4261 ccacgaaact gcgtggtgac cacatctggc aggccaggta gacataggca aatccccagg
     4321 cgagcagagt gaaggtggcg ccagccgcga acaactcgtc ggaggttacc cggtaatcct
     4381 tcatcatgta agccaccagg ctgcctgcgg cgtagaaata caacacggcc tccagcaggg
     4441 atgaccacat caacaggcta gggtttggaa ttaagatcca caagagtgaa agaaggatgg
     4501 caggcgccac gagcacccac gcgatccagt tgagtgccgg actgcgcgtg atgacccaga
     4561 ccaccagtgt caaaatcagg attccgaacg cacccagaat gacacgcccg cttgagtttc
     4621 cgtccaacgc ggcatacaga aaaagactca acaattgcgc agccaacagg aaagcagatg
     4681 gatgggttcg tgcaacagca cgccaatcca cgaaggaagg attatgttta ctcatacctt
     4741 caattgtatc cttgaatgcc tcccacaaat ctgacgatga gagcccgggc tgtgcattca
     4801 atctttattt tgttggattt ggagaggtgc cccgccgaag gcaggcgtat gatctgctca
     4861 atcgcacgac ccgtcacggc cccatagcta ccgcctgcca gcattcctgc gacgagggac
     4921 gtaaaaattt ccacgcctga cgccagaagt acaagatcga tcagcgtcct gcgcgaggca
     4981 ccacacctcg cagcagcggc cattgtccga gcccgatgac ggtccataag aatgtgtgaa
     5041 agatcagatg gattgcaaaa ttttcataag gtgcagtgta cgcgatcaac accggactca
     5101 aaacgcgcac gcctagtgat gcaatcagca aatagccaac aaggccgctg atcgttgaag
     5161 ctggcagcca aagagcatga gcgagtctat tgcgagaacg gagcattgag tagaggccaa
     5221 tggattgccc tagcccaaag aatagcctgg tcaggatcaa tgtccgtgga tgtgtccctc
     5281 gcctgagcag gcctgcaatc atcgcgacga tggtccattg cagccaaaag ggaatcgcga
     5341 aacgagatgc tttcgttcaa atcaaatgcg attctaaatt ttgctcaagt aatttgtaac
     5401 agcttccctg aattcatttg cccattgaaa ggattctatg gcctgttctt ctgtgataac
     5461 aggttctgca tcataatgcc ccgtctcacg gcgtttttgc gccacgacaa tatagcgatg
     5521 aaactttggg tctagcaagc cagtctttgc aaactccttg ccatatgaag ctattagcgc
     5581 ggagtgactc gaaaacttta agccttttga caatagcagg gcctcaacca ggtaaaaatg
     5641 gtgtaataag attgtgcagc agaaaagccg ataaaccctc tgtcgatcaa taattttgct
     5701 gctgcatggc tatcatctgc tttgtccagc aggagttgaa tctcttcttt actcatactg
     5761 cgattccctc tgcccggaca tttagcatga agggcatttg gttttccata taattcttat
     5821 tcgatgcgaa gacccatgaa attagaacat catgtttcaa acagagcgat gagatgaact
     5881 cagatgagcg tttttcagca tctacataat caacatcccc attcaataca atcatcacat
     5941 cgatatccga atcgggtgga tgagcgtcgc cgcgcgcata ggaaccgaag agcacaacag
     6001 tctttaattg gtcgccatag atttcgacga ggcctttttt caattcttgt agaatctttt
     6061 taatatttct aggcatgact gtattttcac ctgtatttta ccctttttca aatcatccaa
     6121 taaatcgcac tttatcatca aggctcctcc tgcggcattt gcgcttgcgt gcactatagc
     6181 tgagccctca tgtatgaatt cgaagattaa aacttatagt ccgagagtta gcctctctgc
     6241 catcttcaca aaaagagccg cccttgcgga cggctcttcc attgttactc aatcatctaa
     6301 tttagaaaac tgttgatcca ggcaagtacg tctgggatat caacacgcga agaatagccg
     6361 actccgctgc agttgatatt ggtcacatac gagttcactc ccaggacgac gttggtgccg
     6421 cgcagcaggt ctggaccgcc tgagtcacca aagcacaacc caccggagcc accacccgga
     6481 ttcaacgcca ggcgcacgaa ttcggcggaa tgcgcgaaat taccggagac caattcactg
     6541 ggagcgtaga ggcgattgta atatccatcc cagcgataga acggtggcgg ctgtggcagc
     6601 agtgagccag ggatctgagc ctggaactgc acgccatagc ccacaaaatc gaccttggtt
     6661 ttgttcttga gcgtatccac cagcccggcc gctggcaaag ctgcaaatcc acctacatcc
     6721 accggttcat ccagaacgac gattccgacg tcacgatagg cgccgcccgg aagcccattg
     6781 ctgttgagcg ggctgttccc ataatcggga ttcgtatgcg gcgtcccttc ggtggactcg
     6841 ccaccgccaa acggaaagtc ggagacgatg aaggagaaat ctgggttctg aagtttctca
     6901 ttgaaccaaa tgcgtgccgc cacggcgcca tccgtacaat gaccggccgt caggactacg
     6961 gtcggagaga gcagcgcgcc gctgcacgtc caggcaggga ccacgcccac gcccgggata
     7021 tctgcatcaa atactaacag accaatatag gggtgcgcgt tcccatccgg ctgaccgttg
     7081 gtgatcgccc ccgcgccgcc ggccagactc aacacaagca tcatcgcaag gaccgatgca
     7141 agcaggactt tcttcatgtg gtactccttt tcaaaatcaa tttggaattg gaaaagcacg
     7201 gacacacgtt cacagaatta agtcggacgc atcacctcct tcccggagaa gaagactgcc
     7261 gaagtgaaac gttcggcagt ggattaaaca tctcgaaatt atgtagagcg cacccttcag
     7321 atgctactca ttcttgttgg aatcaagatg ggaagagaga atgctttgtt gcccctcgaa
     7381 ggaagggttt tagttacaac atttcaattc aaaatcccgg aaagccagtg cattacgttc
     7441 aacacaaact ggaagttctg ttctgccatg ggagcgttca tccctgatgg ctgattatcg
     7501 gtaagttgtg cggtgaacat ggcggcttcc ccaaaaaagg cggcacgccc ggaaccaaac
     7561 ggcatgaccg cgccttgcag ccagccgcca acgggaagcg tttttgtatc gggcgtaaat
     7621 acccaggctt cctcaggcat cagtaaaaca aaatcattcg gcagcaccat caaaggctgt
     7681 gcggactcgg gcgcctgaaa ggcctgcccg gtaaaagatc gaatgcttgt gacggactca
     7741 gtttcattac gcccctgtgt aatcgcgttt ggaatcaacg tattggcatc gacagaaaaa
     7801 atagttggga taccgaaggc cgcgtcgcgg ctcttttcat cgctgaagcc tgccaccgcg
     7861 aaaccatcat tgaactccac cccaaaggcg gaggcgagct tcgctgcgac tccggccagc
     7921 ggcatgtggt ctgcgatcaa taacaatgag cctccgccat tcacccagtc tccgacagct
     7981 acgatctctt catcggtaaa tccggatggt gtgggatatg gatattctcc ccagccatcg
     8041 agttcgagct gagcatttgc aatgaccata atatcgcacc gttccaaaac agccgcgctg
     8101 aatttttcgc tactggattc gacgacatag ccatcacgcc gaagcaattc gccaaaagcc
     8161 cagaatcgat tcttgagcgt gtggaagttg ttatgggctt catcgagaca aacgacaagt
     8221 ccttcacccg ttccataagc agggtcgctg acctgaggtt tgtaggattt atcggggacc
     8281 tgacctgtat tgcaactgac catcatgagc ataactgcaa tggtcagaat aagattagtg
     8341 cgcaatttat ttgatctata tccattctcc tacctggttg gacagcccat tgacgcattt
     8401 gtttctttaa tatagaaaaa cccctcctta gaggggttca aacacaattt tcgctaacac
     8461 tctcccctgc agtcacttca acatacggga gaaggcttat taagaaatgc ggttttttct
     8521 acaactatac ttgaagaagc tcttcgtagg tatccaggga cggttgcccc gcaaataatc
     8581 catcgaacaa ggcaagagtc tgtgctaacg aggcgttcat ggcgtcggca tcttctttcg
     8641 tttcccacag cggaccttga ttgcttgata aatgcctcat caagcatttt tattcaccgg
     8701 aatgcctgtt gcgcggaaaa caagccagta aaaaagagcc gcaaatatca catagactac
     8761 cagcaccaaa aatatgagta aattaaattc attggacagt tgatacctta tcaggagcag
     8821 cgccccaata aagaaatgag caaagtttgc cagcgatacg ggacgcgcat aaatgccacc
     8881 gatcgcactg tctttggctg tccagttcat catcgcgata gaaaaatata aggcgcctaa
     8941 tagctgaatc aacactgaca aaggatttgc catcggcacg cccagcatcg ccaataaaac
     9001 atcgggagca aataaggcga caatgccggt cagcccaagc accagactgc ttgctgtcat
     9061 taataatttt gtgttcatat ctataacctt tgaattctaa ataatacccc ctccctcgca
     9121 acaaaccact tcgccaggtc tccctttgcg aagcacaagt gtatttttcg cctggcgctc
     9181 tttagagccg cgtcattcat aaaaaaaaac tcgacgattc atcgtcgagt ggtcatcacc
     9241 ttcgaactgc tggtgacgct gcgatgatgc gcggcgtaca ccagcggttg agggcagtac
     9301 cttatatgtt accggccagc cagctccggc acaggtataa cgcccagttg ttgcatcatg
     9361 ccgaattcgt caaagatgcc ccagctctcg gcaatctttc cattcacaag gcggtctatg
     9421 gctatgccag tgacagtgga cgacttaccg gtggcgggga tgccagccag ttcaccttga
     9481 tgtgtgccgt gtgctgtcca gcgggtcatg accttatcgc cttcggcgat ctgttcgtcg
     9541 atcgtgaaat gaacgtccgg gaaggcattg cggtacatcg tgacgagttg tttggtgcct
     9601 tcgagtccgg gcagcagccc ggaaggattg ccagggccac tattcacctg gtctttgaca
     9661 ataatctgat ctgccacggc gagattccct ttgctgaaaa cttcgtcgaa gaaacggcgc
     9721 gagacggttt tgttggtttc tgatgacatg ggtaattctc cttatgtgat cggtatgcgt
     9781 ctcctggagg cgcacggaag ggtgctataa gtccgaatat gttgaccccg ttgtggctgg
     9841 acgaacagtt tggtcagagc cgccctttat cgaaaattaa agggccatct gcaaatgacg
     9901 cagacggccc acaagggtaa actgtatcag ctacaatatc aaattgactt atcttctttc
     9961 attctatcac gccctctcga taggtagctg cttcaacgct tcggttcaac acaactcata
    10021 tacttggtca acatctccca caaagcagac tcccccttcc cactgcgaca cttgcacgcc
    10081 agggagagga aagaagggga tataacttat ctacagctac acttgaacca gcacttcgta
    10141 ggtatccagg gatggttgcc ccacaaataa tccatcaaac aaggcaagag tctgttgtaa
    10201 cgaggcgttc attgcgtcgg catcttcttt cgtttcccac agcgtgatgg tcacgccttt
    10261 gccggttccg cggtcgccaa ccagataggc gcccttgaat ccttttacat ccttcaaacc
    10321 cggcgcgacc ttatcacgcc agatggatac cgcctcctct gtcttaccag ctaccagttg
    10381 tgaattgatc attcttgcat acatgtttgc ctcctttggc gatggtgtgc gcccctctga
    10441 acggagccgc acaattggat catcggactc acgttgacct actcactata gcactgttcg
    10501 cgccggtttg ttatagcagg ttttgaggtt caagatatat gtctcacagc aagcttccct
    10561 tcccaaagga taagcggatg ggcgatccat catcacatgg ttctataatc ggtgatggta
    10621 tatacagctt ttactgtttc ttatcgtgct gctcctgctc aagtttcagc tgatcctgtc
    10681 tgcgcaactt tt
//
LOCUS       DASTJP010000018         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1580417, whole genome shotgun sequence.
ACCESSION   DASTJP010000018 DASTJP010000000
VERSION     DASTJP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580417"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1281
                     /locus_tag="VFQ23_00545"
     CDS             112..1281
                     /locus_tag="VFQ23_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9905085.1"
                     /translation="MVGKKILVLDENATSRNFLANTLREKQFKVFEAASGKEALILAW
                     RDEPDLVLFDPVLSDIKEREFIQKLRSDSRTSTIPLIALSSDPSSNRKEACLSAGVNE
                     YFIKSSQALTALEESFSRLFVEPQTFDVPGKNPGLLMVFLSAKGGTGTSSLCANLAMN
                     IKQSQPEASVVVADLVLPIGSIGQIVGYDDRPNLVSVSDLTAAQTNIAYFRNNLPEPE
                     LWQFQLLAGSPDPQHGNVIKGERIEQIVNVLRAAYDFVFLDIGRSLSRISLPLIQKAD
                     LITLIVSTDQSTVQLTRTVWEYLEAQGIDRQKIYAILNRAVGLEGLTKAEAEEIIGLN
                     IKTTLPYMGGNFALANNLHQPITTKYPTDTASIIFKETAGDMARLARRLRAIRIA"
     gene            1418..1717
                     /locus_tag="VFQ23_00550"
     CDS             1418..1717
                     /locus_tag="VFQ23_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905086.1"
                     /translation="MSIEYDEKGKIFTDVVSKIAVYATVQTTTHMMRGHIHVRRDQRI
                     IDELDLNENFLAITDVSVLGNDGQTLFQAPFLALRRTQIVWVIPEQNQTNGEGEF"
     gene            1717..>2071
                     /locus_tag="VFQ23_00555"
     CDS             1717..>2071
                     /locus_tag="VFQ23_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905087.1"
                     /translation="MATPISSNFTRLTSADLLKFKRYLVENVSRSMEGESLQLGDRND
                     IVKQRLQEAYVQTKVSLPDDLRQQVFSEILDEMTGFGPIQPLLDDPDISEVMVNGPKK
                     IFVEKGGKVTKSAVTF"
BASE COUNT          565 a          492 c          490 g          524 t
ORIGIN      
        1 cgtctgcgcc agaagctgga agacgacgct catacaccaa aacatattgt gacgcgctcg
       61 gggctgggat atttgatgcc ccccatcgaa gattgagttt ttggaggttt tatggttggt
      121 aagaaaatcc tcgttcttga tgagaacgct accagccgta attttcttgc aaatactctt
      181 cgcgaaaaac aattcaaggt ttttgaagct gcttcgggaa aagaagcgct catcctcgcc
      241 tggcgcgatg aacctgacct ggtgttattt gacccggtat tatctgatat taaagagcgg
      301 gaatttattc aaaaactgcg tagcgattcg cgcaccagta cgattcccct cattgccctt
      361 tccagcgatc cctcttccaa ccgaaaagaa gcctgcttaa gtgcaggcgt gaacgaatat
      421 ttcatcaaat cttcgcaagc ccttacggcg cttgaggaaa gtttttcgcg cttgtttgtt
      481 gagccacaaa cctttgatgt tccgggtaaa aaccccggac tattgatggt ctttctcagc
      541 gcgaaaggtg gcacaggaac gtcgtcatta tgtgccaatc ttgcaatgaa tatcaagcag
      601 agccaaccgg aggccagcgt cgtggttgct gatctggttt tgccgattgg ctccatcggt
      661 cagatcgttg gctatgatga cagaccaaat ctggtttcgg tgtcagatct cactgccgca
      721 caaacgaata tagcttactt tcgtaataat ttgccggagc cagagttatg gcaattccag
      781 ttgctggcag gttccccaga cccacaacat ggaaatgtca tcaaggggga acgaattgag
      841 caaatcgtga atgtgctccg cgctgcatac gattttgtct ttctggacat tggacgttcg
      901 ctctcgcgca tcagcctgcc tctgatccaa aaggccgacc tcatcactct gatcgtttct
      961 accgaccaga gtacggttca attaaccagg acggtttggg aatatctcga agctcaggga
     1021 atcgatcgac aaaagattta cgcgatcttg aaccgggcgg ttgggctcga gggattaacg
     1081 aaggcagaag ccgaagagat catcgggcta aacataaaaa cgacgctccc ctatatggga
     1141 ggtaattttg cgctggcaaa taacctgcac cagcccatta caacgaaata tccaactgat
     1201 acggcctcca ttattttcaa ggaaaccgca ggcgacatgg caaggcttgc gcgccggctg
     1261 cgtgccatac ggattgccta atgaaagtga aaggcaaaaa ccagtggttt tcgactaaac
     1321 tttgattgca gtccctttcg gcgttgtcaa agcgtaatcg ttattgacaa tgtcctcccg
     1381 ccagagcggc gtttttagca agataagaag aggtattatg tctattgaat atgatgaaaa
     1441 gggcaaaata tttacggatg tcgtttccaa gatagccgta tacgccacgg tacaaactac
     1501 aacacacatg atgcgcggcc atatccacgt ccgtcgggat caacgtatca ttgatgagtt
     1561 ggatttgaac gaaaactttc tcgcgattac cgatgtaagc gtgttaggta atgacggtca
     1621 aacactgttt caagccccat ttttagccct tcgacgcacg caaatcgttt gggtcattcc
     1681 agaacagaac caaaccaatg gggaaggcga gttctaatgg ctactccaat ttcatctaac
     1741 ttcacaaggc ttacttcagc cgacctgctc aagtttaaaa gatatctcgt tgaaaatgtc
     1801 agtcgctcca tggaaggtga atctctacaa cttggcgacc gcaacgacat tgtcaaacag
     1861 cgattgcagg aagcctacgt tcaaacaaag gtttcacttc ctgatgatct ccgtcagcag
     1921 gtattcagtg aaatcctcga tgagatgaca gggtttggac ccattcaacc attactcgat
     1981 gatcccgata tcagcgaagt catggttaat ggcccgaaga aaatttttgt tgaaaaaggc
     2041 ggcaaggtaa cgaaaagcgc cgtcacgttt g
//
LOCUS       DASTJP010000019         5403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_417613, whole genome shotgun sequence.
ACCESSION   DASTJP010000019 DASTJP010000000
VERSION     DASTJP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417613"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VFQ23_00560"
     CDS             <1..1102
                     /locus_tag="VFQ23_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9905088.1"
                     /translation="TLGGSMEKKLRKMVNPMPETKPLRFGIIGCGSASIPVCEAITAS
                     PLTELTAVYDVNHNLANDISQRFHVPMMKSLDELLANPIVDSVYIAVPHYLLASLTQQ
                     SLKAGKHALTEKPMGISLEEIDESTRLAADHKLAMGVFYEMRYAPAHDKARELIQAGA
                     IGKIVGVQIQTLIDKPLSYWQSGYTGRSINPWRSIKAQAGGGVVLMNTSHLLDAITYV
                     TGLRITSVCAEVATLTASVEVEDFATATLRFENGAIGSLISAAHIPGARDDEFCCIYG
                     TEGQLRLPDPYGNNPLQLYLKHQWSDLEAGQWHALQSEAVPVYKSAIEDFARSIQSGE
                     CVRINGHDARYVLSVVLGMYQSAKEQRRISIS"
     gene            1115..2053
                     /locus_tag="VFQ23_00565"
     CDS             1115..2053
                     /locus_tag="VFQ23_00565"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET9905089.1"
                     /translation="MKDIDLRGIIPAIVTPMTNEGELDLPALKRYVQWLADQGPVALA
                     VNVDTGEGPHLTADEKRQTLETVAEAVAGKCKVVGGVAGPSTAQGVANARAAQAAGAD
                     ALLVFPISAFLGQPLNPEVPYRYHAAIAEAVDLPLILFQLQPALGGVLFTTEILHKLI
                     TIPSVTAIKEASFDAMRFLQVKAALDTASRKITFLTGNDNFICESFILGAEGALLGFS
                     TLGTREQVDMLEAVRRGDIEEARELGSRLQPLADIIFAPPVTDYRARTKEALKMLGIL
                     ENTTVRPPLLQIPASEHETIRRSLQNAGLLLSTVSE"
     gene            2320..3750
                     /locus_tag="VFQ23_00570"
     CDS             2320..3750
                     /locus_tag="VFQ23_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9905090.1"
                     /translation="MKSQIRIAFVWLILCSVVIASCAPSAAATPVTVVQTQIVVQTQQ
                     VEVEVTAGAAPVTGEVTVLLIGKPDEDSIDPVSGASIPGIQQLEGMFEAAHDGIDMQI
                     INIPWGSGATGYGPKTESMIQAQEACVYMMPGAFQYGRRGYLENLDPWIANDPNFQDV
                     WPGDYLEQWRGWGPGNPDNQWALPYSGGNRVIHYDSVLFEQWGVEPLSQHPTLEEIEE
                     KAKAMTGTNPVTGEENFGYWYQGKYINWQFQAIAHAFGATWGTVNADGTWTINWDTPE
                     YLQALEWLVMMSQYAPPGALAAEAMPEGFLTDSNVVAIIPEGETGYYLTPFVNDPSLN
                     ERFRTTYNLQGPDGKGGLFIADPVAMAASCPNKEAAWEVMKWLTQSPESQKYNFDSGG
                     NLPVIDGAGEAIPAIGQLVDADAILNQNAFAEIRYPWASAQPRFSLQAAIEAAIAGTL
                     TPKQALEQAQAETEDWLAQQVPTPIP"
     gene            3807..4712
                     /locus_tag="VFQ23_00575"
     CDS             3807..4712
                     /locus_tag="VFQ23_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9905091.1"
                     /translation="MIQKLRKSLIWYAFVAPTFILYGLFVAYPTFETFRLSFFQEVAT
                     RQEFVGALHYVRLLGNQVFLAALLNTAFLGIIFLLIVIPLSLVMASLLNNLRVWPNLF
                     KAIYFLPQITSTVAVALVFSYVFQPNWGLVNGLLRQLGVDQLPLWLAEPRYVLTGSRA
                     AVTILAVWVALGYYMLIFQAGLQAVPAELYDAAVVDGASPMQTWWLITIPSLRPTFIF
                     LYITGTIDAMSRFSDLWMLGGPAGTPARSLQTIVLYMYQTAFESNDFNLASASGVILF
                     VIVLLLTLVNFRSFLGREFSGQSGA"
     gene            4715..>5403
                     /locus_tag="VFQ23_00580"
     CDS             4715..>5403
                     /locus_tag="VFQ23_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9905092.1"
                     /translation="MASSENISPARPVGILRGLLSSQRNFKRVQQVIIFTIMAIGAVA
                     MIAPFAWMLATSFSRSANIAMPRIPTFWPADPSTFNYKVAVTNLPLARYYFNSLIVTV
                     AATLGYLFFSSITGYAFAKGRFVGKSVLFILLLTTLMIPFEIRMIPMYLLMRQLNLND
                     SYAALILPFISGGFGTFLMRQYISAIPDELIDAARVDGASELTIYARIVLPLCGPPLA
                     ALTVLSALWRW"
BASE COUNT         1288 a         1360 c         1387 g         1368 t
ORIGIN      
        1 tacattgggc ggctcgatgg agaagaaatt gcgaaagatg gtcaacccta tgcctgagac
       61 gaagcctctt cgctttggca tcattggttg tggaagcgcc tccattccgg tctgtgaggc
      121 tatcactgca tctcctctca cagaactgac agctgtttac gatgtgaatc ataatttggc
      181 gaacgacatc agtcaacgat ttcatgttcc gatgatgaaa tcgctggacg agttacttgc
      241 aaaccctatc gtggattcag tatatatcgc ggtgcctcat tatctgctgg cttcgcttac
      301 ccagcaatca ctgaaagccg gcaaacatgc gctaaccgaa aaaccgatgg ggatctcttt
      361 ggaagagatc gatgaatcga caaggctggc agcagatcat aaattggcga tgggcgtatt
      421 ttatgaaatg cgctatgcac ccgcgcatga taaagcccgc gaattgattc aggctggcgc
      481 aatcggaaaa attgtcggtg ttcagattca gaccctgatc gataagcctc tgagttattg
      541 gcaatctggt tacaccgggc gatcaataaa cccctggcgc agcatcaagg cgcaagccgg
      601 tggcggcgta gttctgatga ataccagtca tctgctggat gcaatcactt atgtcacagg
      661 tctgcgaatt acgagcgtgt gcgccgaagt tgcgacattg acagccagcg tcgaagtgga
      721 agatttcgca accgcaactt tgagatttga gaatggagcc attggaagcc tgatctctgc
      781 cgcacacatt ccaggcgcac gggatgatga attctgctgc atctatggta cagaaggtca
      841 gctgcgttta cctgatccct atggaaataa tccgttgcaa ctgtatctca aacatcagtg
      901 gagcgatctc gaagccggtc aatggcatgc gcttcaaagt gaagccgttc cagtttacaa
      961 aagcgcgatt gaagactttg cccgttcaat tcaatcggga gagtgtgtgc gaattaatgg
     1021 tcacgacgca cgctatgtat tgtctgtggt gttagggatg tatcaatctg cgaaagaaca
     1081 aagaaggatt tcaatttctt aaagcgaggt aattatgaaa gatatcgact tacgcggcat
     1141 tattccggcc atcgtgactc ccatgacaaa tgaaggagag ctagatttac ctgctctgaa
     1201 acgctatgtg caatggttgg ccgatcaggg accagtagca ctcgccgtaa acgtggatac
     1261 aggggaaggt ccacatctga ctgcagacga gaaacgtcaa acgttggaga cggtagcaga
     1321 agccgtggct gggaaatgta aagtagttgg aggtgttgcc ggaccatcta cagcgcaggg
     1381 tgtggcaaac gcgcgggccg cccaggcagc aggagcggat gcgcttcttg tctttccgat
     1441 ctccgcgttc ttaggacagc cactcaaccc tgaggttccc tatcgctatc acgctgcaat
     1501 cgcagaggct gttgacttac ccttgattct cttccaactc cagccagccc ttggaggagt
     1561 cttattcacc accgaaattc tacacaaact cattactatt ccttcagtga ctgcaatcaa
     1621 agaggcatcc tttgatgcga tgcgcttttt acaggtcaag gctgcgctgg atactgccag
     1681 ccgaaaaatc accttcctga ctggtaacga taatttcatc tgcgagagct tcattctggg
     1741 tgcagaaggg gcattattag gattcagcac acttggaacg cgtgaacaag tcgatatgtt
     1801 ggaagcagtt cggcgtggcg acattgaaga ggcacgcgaa ctaggctctc gccttcaacc
     1861 cctggctgac ataatctttg ccccacctgt gaccgattac cgggcacgca ccaaggaagc
     1921 attaaagatg ttaggaattt tggaaaacac aacggtccgt ccccctcttc tgcaaattcc
     1981 cgcgtctgag catgaaacca ttcgacgctc gttacaaaat gcaggattgc tgctctctac
     2041 agttagcgaa taagtagaga gactcaaagt ccctggtcaa agtgtacttt gagtctctct
     2101 gagcaagaaa ccggaacacg gtcatcaggg tgtcaggctt ggccagcctt ttcatcactc
     2161 gaagacaaag ggttccagtc ccagctaatt gtagcagagg aaccacaacg aaaacaacgc
     2221 cttggaacga tctatcgaaa ggagatatgg ctactcacat ccggttgaat caattgtcgt
     2281 ttgtgtcact ttcaatactt aagacaaaga ggagtgagca tgaaatcgca aattcgaatc
     2341 gcgtttgttt ggttgattct ctgtagtgtc gtaatcgcca gttgtgcccc atctgctgcg
     2401 gctactccag taaccgtagt gcagactcag atcgtagtac aaacacaaca ggtggaagtg
     2461 gaagtaactg ccggggcagc cccagtcacc ggagaagtga ccgtcctgct gatcggcaaa
     2521 ccggatgagg atagcattga tccggtcagt ggagcaagca tccccggcat ccagcaattg
     2581 gaaggtatgt tcgaagctgc ccatgatggc atcgacatgc aaattatcaa cattccctgg
     2641 ggctcaggtg caacaggtta cggtccgaaa accgagtcta tgatccaggc gcaggaagcg
     2701 tgcgtttaca tgatgcctgg cgcattccag tatggccgcc gcggttattt ggaaaacctg
     2761 gatccctgga tcgctaacga tcccaatttc caggatgtat ggccaggcga ttatctggaa
     2821 caatggcgcg gctggggtcc gggcaacccg gacaatcaat gggcgcttcc atacagtggc
     2881 ggcaatcgcg tcatccatta tgactcagtg ctgttcgagc aatggggcgt cgaaccttta
     2941 agtcagcatc ccaccctgga ggagatcgaa gagaaagcca aagccatgac cggtacaaac
     3001 ccggtaactg gtgaagaaaa cttcggttac tggtatcagg gtaaatacat caactggcag
     3061 ttccaggcca ttgcgcatgc tttcggtgcg acctggggca ccgtcaatgc ggatggaacg
     3121 tggactatta actgggacac cccagaatat ttgcaggcgc tggaatggct ggtcatgatg
     3181 tcgcaatatg cacctccggg agcgctcgct gctgaggcta tgcctgaagg tttcctgacg
     3241 gactcaaacg tcgtcgcgat catccccgaa ggagaaaccg gctactatct gacaccattt
     3301 gtgaatgacc cgtcgctcaa tgaacgcttc cgcaccacct acaatcttca aggcccggat
     3361 ggcaagggtg gcttattcat tgctgacccg gtggctatgg cagccagttg cccgaacaaa
     3421 gaagccgcct gggaagtcat gaaatggctt acccagagcc ctgaatctca gaagtacaac
     3481 ttcgattcgg gaggcaatct gccagtgatt gacggtgcag gtgaggcgat tccggctatt
     3541 ggtcagctgg ttgatgcaga tgcgatctta aatcagaatg cgtttgctga gatccgctat
     3601 ccctgggctt ctgcacaacc tcgtttctcg ttgcaggcag cgattgaggc ggccattgcg
     3661 ggaacgctga cgcccaagca ggcactggaa caggctcagg ccgaaactga agattggctc
     3721 gctcagcaag tacccacacc tattccataa gactcatcga agcatggact acgcactttt
     3781 agtgcgtagt ccattatgag gaagatatga tacaaaaact tcgcaagagt ctcatctggt
     3841 atgccttcgt ggcaccgacg tttatcctgt acgggctctt cgttgcttac ccaacatttg
     3901 aaacctttcg gttgagtttc tttcaggaag tagcaacccg tcaggaattc gttggagcgt
     3961 tgcactatgt gcggctgctg ggtaaccagg tcttcctggc tgctctgctc aacacggcgt
     4021 tcctgggcat tatcttcctc ttaatcgtca ttccgctttc gctggtgatg gcttccttgc
     4081 ttaataattt acgagtgtgg ccgaaccttt tcaaggcgat ttacttcctc cctcaaatca
     4141 catcgactgt cgcagtggcg ctggtattca gttatgtatt tcaaccgaac tggggattgg
     4201 ttaacggttt gctgcgccag ttaggtgttg accaactccc tctttggctg gctgagccgc
     4261 gctatgtgtt aacaggttcg cgtgccgcgg ttactattct tgccgtgtgg gttgcattgg
     4321 gatattacat gcttatcttt caggctgggt tacaagccgt gcccgccgag ctctacgatg
     4381 cagccgttgt ggatggggcc agcccaatgc agacctggtg gttgattacc attcccagcc
     4441 tgcgtcccac tttcatcttt ctttacatca ccgggacgat cgatgccatg tcgcgtttca
     4501 gtgatttatg gatgttgggt gggcctgcag gcactccggc tcgttcattg caaacaatcg
     4561 tcttgtatat gtatcagacc gccttcgaaa gcaacgattt caatctagcc tctgcttctg
     4621 gcgtgatcct gtttgttatc gtccttctcc tcacgctggt caattttcgg tcttttcttg
     4681 ggcgtgagtt ttccggtcaa tcaggagcat gactatggct tcttctgaaa acatttcccc
     4741 agcacgtccg gtaggtatcc tacgcggctt attgagttca caacgcaatt tcaaacgcgt
     4801 ccagcaggtg atcattttta cgatcatggc aatcggagcc gtggcaatga tcgcgccctt
     4861 cgcatggatg ctggcaactt cattctcacg gtcagccaac attgctatgc cgcgcattcc
     4921 aaccttttgg cctgctgacc cgagtacatt caattacaaa gtggcggtta cgaatttgcc
     4981 cttggcacgg tattacttta acagtctgat tgtcacggtg gctgcgacgc ttggctacct
     5041 tttcttcagt tccattaccg gatacgcctt tgcaaaaggc cggtttgtgg gtaaatcagt
     5101 cctcttcatt ctccttctca cgacgctcat gattccattt gagattcgaa tgattccgat
     5161 gtacctgttg atgcgtcagt taaacctgaa tgactcatac gcggctctga ttttgccatt
     5221 catctccggt gggtttggga cattcctgat gcgacaatat atcagcgcaa ttccggatga
     5281 attaatcgat gcagcacgag tggatggcgc gagcgaattg acgatttacg cgcgcatcgt
     5341 tttgccatta tgtggtcccc ctctggccgc gctcactgtg ctcagcgcgc tatggcgctg
     5401 gaa
//
LOCUS       DASTJP010000020         6850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_733852, whole genome shotgun sequence.
ACCESSION   DASTJP010000020 DASTJP010000000
VERSION     DASTJP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733852"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..2107)
                     /locus_tag="VFQ23_00585"
     CDS             complement(275..2107)
                     /locus_tag="VFQ23_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905093.1"
                     /translation="MPTGSEQFPAQQVQVSPTTIADATAATQASPLEAPVVPAKPSFD
                     IRLTTVTSGATIIRSAGDALEVPQDQTTDVEVNEGIEVVKSAGQEGQSYSILDFADDL
                     EVELFGDTSVFLAELREQASGSTYANLHLSDGHIFVHLNAQKDASISVKTIQATIRSL
                     TNGAEFDVCQTQDRTCILVKKGVVEVTPQNGKIFLVKVGEAAHVLQEQRPSPPICAPL
                     AELTDWENAYRQVANTPALDQQLAALPQEPCPVTDTGIPVTARILYQDEFWNPFSGWD
                     RGKIGNFNVRYAGLQYYRVQALSLANQFRASVPNELQYTDVNIDLKAIGETVSGGNFR
                     YGLVFRQTDDQYYAFVISPLSKDWYFLKNSSAGQETLVQGTDERMRGFENKETLRVET
                     YGSTFLLFINDRFVDLVSDSEYASGEVGLFVDAIESPDAIVRFDSITIWDMPAVVEPD
                     SAGGREYCYNGFDDDGDRLADRADPDCQRLDRTATPAPISTNIPAQATNTSVAPNTAT
                     PLPSETSIPPTPLPTDTSTPRPTRRPTFTPTNPPPPTATDQPTATTVPPTVEPPTVEP
                     PTVEPPTVEPPTVEPPTAEPPPTEPPAPTEPVVDPTEPPVVPTP"
     gene            complement(2540..2866)
                     /locus_tag="VFQ23_00590"
     CDS             complement(2540..2866)
                     /locus_tag="VFQ23_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905094.1"
                     /translation="MALTLPPDFKEFLKLLKEHDVRYLLIGGYAVGYHGYVRATEDMD
                     IWVAVHPDNAEKLVKTLKAFGFNDPNITPELFLQKPKIIRMGFPPMRLTNVMLLELWT
                     KLIVSK"
     gene            complement(3122..4954)
                     /locus_tag="VFQ23_00595"
     CDS             complement(3122..4954)
                     /locus_tag="VFQ23_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9905095.1"
                     /translation="MTTFISYSRAHSEFVVHLAKDLKSAGFDVWLDQLDIPKGARWDD
                     AIEAAVERSSTFMIVLTPESIESQNVKDELSYAIDSGKNILPVVIKPCKIPLRLRRFQ
                     FVDFTDKPYKESLSDIKRLLSNTQRRLQAYADEMENEAEPSLSSPGVTGPLIKKEPEF
                     SNESLRGDTSRKANSAGRFIVPAIVAALALVAAVAAWNFFANQNAASPPSTDFTVTSS
                     APAISATATPNPAPVEIIDEARVTMRLVSAGDFMMGSDHGMEDEKPAQTVFLDTAYVD
                     KYEVTNAFYRVCVEAGTCEPPAEQTEYFDNPEFDNYPVVNVNWEMAKTYCEWRSARLP
                     TEAEWEKAARGTDGRTYPWGEEISCNEANFFGCEGNPASVSSLVNGRSVYGLYHMAGN
                     VYEWVSSLYKAYPYDRFDGREDLSAAGERVIRGGSWLNSDTENEVRTARRHMADPTTF
                     SENIGFRCAFSAAIPETGALLTITPLNGVTGTHVAATIHSVKTREARAANSTSTLSVG
                     TAALTPQPSAITTPRPGTTATSGAGAPVTEPPVTEPPVTEPPVTEPPVTEPPVTEPPV
                     TEPPVTEPPVTEPPVEPPPTDPPVEEPPPPTEPIVEPPPPTAPP"
     gene            5304..6581
                     /locus_tag="VFQ23_00600"
     CDS             5304..6581
                     /locus_tag="VFQ23_00600"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9905096.1"
                     /translation="MPAFISYSRANSDFAVRLAKDLKSSGYNVWLDQLDIPTGTRWDD
                     EIELALEACTTFIIILSPESLKSQNVKDEVGYAIDAGKDILPVLIKSGDVPFRLRRFQ
                     YVDFTNKPYDASLKEIKSLLAPEGHLVDSQNVEKNPIDGESDRPTKKIKAVGGASSAK
                     PEAIPSPSARRKVPAGLFIGIIALVGVLIAAAIVAATRANRPTETTLADEVSPAEILP
                     TEQPTASPTPETTPSEDVQPETFLTKFMESTDLNEWDHFVNGLGSRKKVEIKPSNDGL
                     LFHLEDQDLYTYYMYKPATFDNVIIRMKAENLGQNRNYVSLVCRRTGDQWYEFSVTGG
                     GDWHLYNHSAEYKGITNGGTFAIKYGKTINEYEMRCIGNDISLLINGQLVQTVDMTPY
                     RNNYTEGQVGFGVTAREVYPIDIKVIEFEVSKP"
BASE COUNT         1637 a         1673 c         1786 g         1754 t
ORIGIN      
        1 cttctaaagt ctctttatga ataagaagaa tcctgattac ctgatagttt tagatttccg
       61 gcgagttcgg tccgtcgagt tgcccggagt gcaggcccag gctgtaccga gagcatagat
      121 tttcaagaaa tcttttgttt gcctgattcc gtgattgatg gtttattaaa atacggtctt
      181 cacccaaaca aataaaaatc cgagtctttg cgaagtaagg gtagcgcaaa aactcggaat
      241 attctttaat tgaactaacg atcactgact tgggttaagg tgttggtact accggtggct
      301 cggtcggatc gactaccggc tcggttggtg ctggtggctc ggttggcggt ggctcggctg
      361 tgggcggctc gacggttggt ggctcaacag tcggtggctc gacggttggt ggctcaacag
      421 tcggcggctc aaccgttggc ggtacagtgg ttgcagtcgg ctgatcggtc gcagttggtg
      481 gtggcggatt ggttggtgta aaggttggtc ttctcgtagg tcgaggtgta gatgtatccg
      541 ttggcagtgg agtaggaggg attgacgtct ccgatggtag tggcgtagcc gtatttgggg
      601 ctaccgatgt atttgtcgct tgcgcgggta tgttggttga gatcggtgct ggtgtagcag
      661 tacgatccaa tcgctggcaa tcggggtcgg ctctatctgc caggcggtca ccgtcatcat
      721 cgaagccgtt gtaacaatat tctcttccgc ctgctgaatc gggctcaacg acggcgggca
      781 tatcccagat ggtgatcgaa tcaaatcgga caatcgcatc cgggctctct atggcatcga
      841 caaacagacc gacttctccg ctggcatatt ccgaatcgct caccagatcc acgaaccggt
      901 cgttgatgaa aagcaggaag gttgagccat aggtttcgac gcgcagggtt tccttgtttt
      961 caaaaccacg catgcgttca tcggttccct gcaccaaagt ttcctgcccg gcagaggaat
     1021 tttttagaaa ataccagtcc ttcgaaagtg gcgagatgac gaaggcgtaa tactgatcgt
     1081 ccgtctgacg gaagaccagt ccatagcgaa agttaccacc actgaccgtc tcgccaatcg
     1141 ctttgagatc gatgtttacg tcggtgtatt ggagctcgtt gggtaccgag gctctgaatt
     1201 gatttgccag ggacaatgcc tgaacacggt aatattgcag ccctgcatag cgaacattaa
     1261 aattaccgat cttgcctcga tcccacccgc tgaacgggtt ccagaattca tcctgataga
     1321 ggatgcgggc cgtgaccgga attcccgtgt cggtcactgg acagggttcc tggggtaagg
     1381 cagctaattg ctggtcgagc gcgggggtgt ttgcaacctg gcgatacgca ttttcccaat
     1441 ctgttaattc ggcaagcggc gcacagatcg gaggggatgg acgttgctcc tgcaggacgt
     1501 gagcggcctc tccaactttg accaggaaaa tcttcccgtt ctgcggagtg acctccacga
     1561 ctcccttttt gaccagaata caagtccgat cctgggtttg gcatacatca aattccgcgc
     1621 cattggtgag gcttctgata gtggcctgga tcgtcttgac gctgatactg gcatccttct
     1681 gggcgtttag atgtacaaag atgtgcccgt cgctgaggtg caggttggca taggtggaac
     1741 cgctggcctg ttccctgagc tctgccagaa acacagacgt atcgccaaag agctccactt
     1801 ccaggtcatc tgcgaagtcc agaatgctat agctctgtcc ctcctgccct gccgacttaa
     1861 cgacttcgat gccctcattt acctcaacat cggtcgtttg gtcttgtggg acttcaagcg
     1921 catcgcccgc gcttcggatg atcgtcgccc ctgaagttac ggtcgttaag cgaatgtcaa
     1981 atgaaggttt cgccggaaca accggagcct ccagtgggga ggcttgggtt gccgctgttg
     2041 catccgcgat tgtcgttggg cttacctgaa cctgctgtgc agggaattgc tcagagccgg
     2101 ttggcacttg ggagccgcac gccgacaata tgatgacaat aattaataag accgttggcc
     2161 taaaagaaag ccagaaattt cgttctatgt tctaccttct ctccggtatg acttccaagt
     2221 ttaatgtgta gcctacgggc cgcgatgtat cggtattggg ggctgatttt attctgggat
     2281 taacaaatat gcaagcttaa aagcatgacg agcgggttaa gagtttgtta caaggtgaaa
     2341 gtacgtcggc gtgccactgc gacgtacaaa acaggtcgga ctagcagttc gacctgctcc
     2401 tttacaccag cttcgctatc gaattttctt ggaactctgc tatttggtta gtctcaaggt
     2461 aattgttcca aatcggcttt atcttttaat ctcccactcg cattcttatt cctcttcagg
     2521 ttttccaaat ctatcacgtt tacttcgata ctatcaattt tgtccacaat tctaaaagca
     2581 taacattcgt caacctcata ggaggaaatc ccatgcgaat aatttttggc ttttgaagga
     2641 ataattctgg ggttatattc gggtcattga acccgaaggc tttgagcgtc ttcacaagct
     2701 tttcggcgtt gtctgggtga accgcaaccc aaatgtccat atcctctgtg gctcggacgt
     2761 acccatgata gccaactgca tagcccccaa ttaacaaata gcgaacatca tgttctttca
     2821 gcaatttcaa gaactctttg aagtcgggcg gtagcgtgag cgccataatt gattctccgc
     2881 aataattcca tgtgctgcag acgttcgaga ggagttctgg aaagccaata ttcccggtct
     2941 tcccggtcag cctcttcaaa agaagaaaca atcgataagg cagttctatc taacttgggg
     3001 aattggattt catccatata ataagtatag cacagcaaaa acaggtcgga ctagcagatc
     3061 ggcctacgtt atttacaaga acgccgagtt atttacactc ggcgttcttt ttcgttgaag
     3121 attaaggtgg cgccgtcggt ggtggcggct caacgatcgg ctcggttggt ggtggtggct
     3181 cctcgactgg tggatccgtc ggcggcggct cgactggtgg ctcggtgacg ggtggttcag
     3241 tgactggcgg ttcagtgact ggtggctcgg taactggcgg ttcagtgacc ggtggctcgg
     3301 taactggcgg ttcggtgact ggcggttcgg tgactggcgg ctcagtgaca ggagctcctg
     3361 cccctgatgt agctgttgta cctggtcgcg gggttgttat tgcactgggt tgtggggtca
     3421 gcgcggcagt acctacggaa agtgtggagg tcgaatttgc cgctcgtgct tcacgcgttt
     3481 tgaccgaatg gatggtcgcg gccacatggg ttccagttac accattgaga ggcgtgatcg
     3541 taagcaatgc gcctgtttcc ggaatcgcag cggagaaggc acaacgaaag ccgatatttt
     3601 cgctgaatgt cgttgggtcc gccatgtgac ggcgggcagt ccgaacttcg ttttcggtat
     3661 cggaattgag ccaggagccg ccgcgaatga cgcgctcgcc ggccgcgctg agatcttccc
     3721 ggccatcaaa acgatcatag gggtatgctt tatacaggct gctcacccat tcatacacat
     3781 tgcctgccat gtgatagagc ccataaacgc tccgtccgtt caccagactt gaaacggatg
     3841 caggattccc ctcgcagcca aaaaagtttg cttcattaca gcttatctct tcaccccacg
     3901 gataggtccg cccgtcggtt ccgcgcgcag ccttttccca ctccgcttct gtaggcagac
     3961 gtgcgctgcg ccattcgcag taggtcttgg ccatttccca atttacgtta acgacaggat
     4021 aattatcaaa ctccggattg tcaaaatatt ccgtttgttc tgccggcggc tcacatgtgc
     4081 ccgcctcgac acatacccgg taaaaggcat tggtcacttc atatttgtct acgtatgcgg
     4141 tgtcgaggaa aaccgtttgg gcgggctttt catcttccat cccatgatcg ctgcccatca
     4201 taaagtcacc cgcacttacc agccgcatgg tgacgcgcgc ctcgtcgatg atttcaaccg
     4261 gagctgggtt gggtgtagct gtggccgata ttgctggggc gctggatgta acagtgaagt
     4321 ctgtagaggg aggggaagcc gcgttctggt tggcgaagaa gttccaagcg gcaaccgccg
     4381 ccacaagcgc aagcgcagca acgatcgctg ggacaataaa cctgccagcc gaattggcct
     4441 ttcgagatgt atctccccta agcgattcat tggaaaactc tggttccttt ttgatcaggg
     4501 gaccagtaac tcctggagac gaaagtgatg gttcggcttc attctccatt tcatcggcat
     4561 aggcttgcag acgtcgttgc gtattactta ggagccgctt gatatccgaa aggctttcct
     4621 tatagggctt gtcggtgaaa tcgacgaatt gaaatcgcct taaccggaga gggattttgc
     4681 agggtttgat aaccacaggc aagatgttct tgcctgaatc aatggcatag ctgagttcat
     4741 ctttgacgtt ctgagactcg atcgactcag gcgtcaacac gatcataaat gtggagcttc
     4801 tctccacggc cgcctcgatg gcatcatccc agcgtgcacc tttagggata tccaactggt
     4861 caagccatac atcaaacccg gcagatttta gatccttggc gaggtgaaca acaaattctg
     4921 aatgagcccg cgaataggaa ataaaagtag tcatatcgac ctcctaaatc acccaaacct
     4981 atttcctgga acgaaacaaa tttatcataa atgggttctt attacaagaa tttcagctca
     5041 ttttcatggg aatggacgga agtcttgttt aaaaacttcc agcattcgtc gttggagtta
     5101 tgatcaaaat cgatcggtgt tctgtgacgg catcgaatag acaatgggag tgtctaattt
     5161 cttggggacc gtggcgaaac agcgattagc ccattctttt gaacactccc cagacaacga
     5221 agttgtgaag catcttgttg cacttgctaa agtgtgataa attcactgcg tgtaataagg
     5281 caggaagaca ggaggaagtt agaatgccag cgttcatatc ctattcaaga gccaactccg
     5341 atttcgcggt gcgccttgct aaagacctga aatcatcggg ctataacgtc tggctggatc
     5401 aactcgatat acccaccggt acgcgctggg atgatgaaat agaactggct ttggaagcct
     5461 gcacgacatt catcatcatt ctttctccag aatcactcaa atcgcaaaac gttaaagatg
     5521 aagtgggata cgccatagat gccggcaaag atatcctgcc ggtcctcatc aaatcgggag
     5581 atgttccatt ccgactgaga cgtttccagt atgtagactt taccaataag ccctacgacg
     5641 cgagtcttaa ggaaatcaag agcctcctgg ctcccgaggg tcacttggtc gattcacaga
     5701 atgttgagaa aaatcctatt gacggtgagt ccgaccggcc cacgaaaaag ataaaagcag
     5761 tgggaggcgc ctccagcgca aagcccgaag ccattccttc tccttctgca aggaggaagg
     5821 tgcccgccgg actttttatc ggaatcattg cactcgttgg tgttctgatt gcagctgcta
     5881 tcgttgctgc cacacgagcc aatcgtccaa ctgaaacgac tcttgccgac gaagtctcac
     5941 ctgctgaaat ccttcccacc gaacaaccaa ctgcaagccc aacacctgag acgacgcctt
     6001 cagaagacgt gcagccagag accttcctca cgaaatttat ggaaagcacg gatctaaatg
     6061 aatgggatca ttttgtaaat ggcctgggga gtagaaaaaa ggtagaaata aagccatcga
     6121 atgacggtct tttgttccac ctcgaagacc aggatttata tacctattac atgtacaaac
     6181 cagctacctt tgacaatgtg attattcgaa tgaaggctga aaacctgggt caaaaccgca
     6241 actatgtcag cctggtttgt cgcagaaccg gtgatcaatg gtatgaattt agtgttacgg
     6301 gtggtggcga ttggcacctg tataatcata gcgccgaata caagggcatt accaatggtg
     6361 gaacgttcgc tataaaatat ggaaaaacaa tcaatgagta tgagatgcgt tgcattggga
     6421 atgatatttc actacttatc aatgggcagc ttgtgcaaac tgttgacatg actccctatc
     6481 gaaataacta tacggaaggg caggtgggat tcggagttac ggcacgcgaa gtctatccca
     6541 ttgatatcaa ggtcattgaa tttgaggtct ccaaaccata gtccaaaaca accatggatg
     6601 gaataaaaaa tctccgatta cacaagtaat tcggagattt gtgtttccta cacctagatc
     6661 ttaccagcgc ctgcacgctt cgcgctgcgc ggcctcggtg ccatcgctga cgcggtgatc
     6721 gactgaaatt gtcgctttca cgcagagcgg ccagccgggc cttttacacc aacatcctca
     6781 caggattttc caggaaccca gctaaagcct gcatgaactg cgcggcctct gcgccatcgc
     6841 tgacgcggtg
//
LOCUS       DASTJP010000021         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_840097, whole genome shotgun sequence.
ACCESSION   DASTJP010000021 DASTJP010000000
VERSION     DASTJP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840097"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1170)
                     /locus_tag="VFQ23_00605"
     CDS             complement(28..1170)
                     /locus_tag="VFQ23_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905097.1"
                     /translation="MSKRLAVSFVVMITLVALVGSMLASASVAIAAPPPKPTKTPTPG
                     PGQLTPIQIYGTWHCGNDACTWASVRTVSEFDSKNQWLIDRGDGQPSVNLVVLSFVNP
                     LKLLNKTTDAGNLNGVPIGMTQEIVNYFKSRNIRVMLSIGGITYTDDWNTALATNPTQ
                     FGLNAAEVAENLGVGIEIDYEENTSPNLTGLQAFINAYRSVHPYDPTGNNHAARLTID
                     LAAGDRWLIALTRKATADWLNTTTPVLDYANAMVPSRQPSTSGAIANWQEHVDGKAQY
                     DPPILPLAPAKFTGSLYVVSGTNIAPECNNFANSLQNSTGTFVRTVSPNGAGTTSGML
                     GYMFWAAEMPSTRRITTAPPNSCEGGVGVGATTYNIPIPMPALRQQ"
     gene            complement(1263..2942)
                     /locus_tag="VFQ23_00610"
     CDS             complement(1263..2942)
                     /locus_tag="VFQ23_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET9905098.1"
                     /translation="MPRRSLARLYKDEFSGYSLAKFQQDLLAGLTVAAVALPLALAFG
                     VASGATAAAGLVTAILAGIVMGLLGGAPYQISGPTGAMSAVLIVLVSRYGLEGIWVAG
                     LFSGALLLVIGLLRLGRFIAFIPSPVISGFTSGIALIIFIGQIDNFLGIQTPAAETAA
                     QKLIGYFNSAFTINSLSLILGLVVVGTMLLWPSALNARFPSSLLGIILATLLNSILSW
                     STQTIGEIPQTLILQSRLYPSAIPWNQLSDFVAPTLTITALGAVESLLCGAVGSNMTG
                     IRLQANQELIAQGVGNMLIPFFGGVPATAAIARSSVGIKSGGQTRLVSIIHALGLLLS
                     MFLLTPFMERIPLAALAGVLMVTAVRMNEWDAIRFIFGKRFKTDMIAFTVTMLATVVL
                     DLTQAILIGSILAGAVFLNKIASIEVEVQEVDIERLRQKGIDTAGRCNHVRVAFLTGP
                     LFFAATGQFNEAFADLKETHALILSMRGVPLIDTAGIEAIHRLHKRLHDQGGTLMFAG
                     VHDNARQMMERGGLIEAMGEENFFWSSDQAIVEAEKRGCKFCVFTTIAPKG"
     gene            complement(3036..>3284)
                     /locus_tag="VFQ23_00615"
     CDS             complement(3036..>3284)
                     /locus_tag="VFQ23_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905099.1"
                     /translation="ASISYESFGIPLDAAGKVKARAMNNIEPAVQLTGNNAIVELITF
                     TKWGGFYRQTFTISRAFPHTIVDTQSENIVPYDCGIAF"
BASE COUNT          868 a          829 c          841 g          746 t
ORIGIN      
        1 cttcgcgaag tgactgtcac ctttaaacta ctgttgtctc aatgcaggca tcgggatggg
       61 aatgttgtag gtagttgcgc ccaccccaac gcctccctca caggaattgg gtggtgccgt
      121 ggtgatacgc cgcgtggaag gcatctctgc cgcccagaac atatagccaa gcatcccgga
      181 tgtagtcccc gcgccattgg gtgacacagt gcgcacaaac gtgccagtgg agttctggag
      241 cgagttggca aaattattgc attccggcgc gatgttcgtt ccactcacaa cgtaaagact
      301 gcctgtgaac ttggctggag ccagaggcag aatgggaggg tcatattgag ctttcccgtc
      361 cacatgttcc tgccagttcg cgatcgcacc ggatgtactg ggctggcggc ttggcaccat
      421 agcattggcg taatcgagaa caggagtagt ggtgtttaac caatccgcgg tcgcctttcg
      481 ggtgagcgcg atcaaccaac ggtcgccggc agcaaggtca atggtgaggc gtgcagcgtg
      541 gttattgcct gttggatcat aggggtggac cgaacgatac gcattaatga acgcttgcaa
      601 gcctgtaaga tttggactcg tattttcttc ataatcaatt tcgatgccga caccaagatt
      661 ttcggcaact tctgcggcat tcaagccgaa ttgagtcggg ttggttgcca aagccgtgtt
      721 ccaatcatca gtataggtaa tcccaccaat agaaagcatt actcgaatat tgcgactctt
      781 aaagtaattc acgatttcct gagtcattcc aatgggaacg ccatttaagt tacctgcatc
      841 ggtcgtttta ttgagcagtt taagagggtt gacaaagctt aacaccacca ggttcaccga
      901 aggctgtcca tccccgcgat cgatcagcca ttgattcttt gagtcaaatt cactaaccgt
      961 acggacagat gcccaggtgc aggcgtcgtt accacaatgc catgtcccat aaatttgaat
     1021 gggggtgagt tgacccggac ctggcgtcgg tgtcttcgtc ggtttgggag gtggcgcggc
     1081 aatcgcgaca cttgcagaag caagcatgga accaaccaaa gcgaccaggg taatcatcac
     1141 tacaaacgat acagccagcc ttttgctcat atacgctcct cgggatagga aataacgtgc
     1201 cgtaattatt attgcatttc tcccttaccg agtcaacgca aatttccagt tacataaatt
     1261 ttctaacctt taggagcgat ggttgtaaag acacagaatt tacagcctcg cttctccgcc
     1321 tcaacgatgg cctggtcact ggaccaaaaa aagttttctt ctcccatagc ttcgatcaat
     1381 ccgccgcgct ccatcatttg ccgcgcattg tcgtgcacac ctgcaaacat gagcgtgccg
     1441 ccttgatcgt gcaatcgttt gtgcaaacgg tgaatcgcct cgatgcccgc tgtatcaatc
     1501 agcgggacgc cgcgcattga gagtatcaag gcgtgtgttt ctttgagatc agcaaaggct
     1561 tcattgaact gacctgttgc cgcaaagaaa agcgggcccg tgagaaacgc aacgcggaca
     1621 tgattacatc tacccgcagt gtcaatacct ttttgcctga gccgctcgat atccacctcc
     1681 tgcacttcga cctcaatact ggcgatctta ttcaggaaga cagcaccagc aaggatagag
     1741 ccgatcaaaa tcgcctgggt taaatccaga acaactgtag ccagcatggt gactgtaaaa
     1801 gcgatcatat ctgttttgaa gcgtttgccg aagataaacc ggatcgcgtc ccattcgttc
     1861 atgcggacag cggtgaccat cagcacacca gccagggcag cgagaggaat gcgttccatg
     1921 aatggcgtaa gcaaaaacat tgaaagcaat aatccgagag cgtggatgat actcactaat
     1981 cgcgtctggc cgcctgattt gatgccaacg ctggagcgcg cgatcgcggc tgtggcaggg
     2041 actccgccaa agaacgggat gagcatgttg cctacaccct gcgcgatcag ttcctgattc
     2101 gcctgtaaac gaatgcctgt catatttgat cccactgcgc cacacaataa ggattccact
     2161 gcacccaggg cggtgattgt caaggtcggc gcgacgaaat cggaaagttg attccatggg
     2221 atggctgagg gatatagacg agattgaaga attagagttt gggggatctc accgatcgtt
     2281 tgtgtggacc agctcaaaat cgagttaaga agcgtggcaa ggatgatccc caggagagaa
     2341 gacgggaatc gtgcgttcaa agctgaaggc cataagagca tggttccaac aacgacaagc
     2401 cccagaatta gggaaagaga attaatggta aatgcactgt tgaaatatcc gatcagtttt
     2461 tgcgcggcag tttcagcggc gggggtctga atgccaagaa aattgtcaat ttgaccgata
     2521 aagatgatca gggctatgcc agacgtgaaa ccgctaatga cgggcgaagg gataaatgca
     2581 ataaaacgac ccaggcgaag cagtccaatc acaagcagaa gggccccaga aaacaaacca
     2641 gcaacccata tcccctccaa accatagcgc gaaacgagca cgatcagcac tgccgacatt
     2701 gcgcccgtcg gcccggaaat ttgataaggc gcaccaccca gcaaacccat aacgatacca
     2761 gccaggatcg ccgtcaccag gcctgctgcg gcagtggcac ctgaggcgac tccgaacgca
     2821 agtgcaagtg gaagcgccac tgcggcaacg gtgagcccgg caagcaggtc ctgctgaaat
     2881 ttggcaagtg aatatcctga aaactcgtcc ttgtaaaggc gagcgaggct tcggcgtggc
     2941 atatgaaatt ctccaaagaa aatagtttta caaaggctcg atgctccccc aaggcgtcct
     3001 ttgttcaata ggtattaaga ttttatcatg agtttttaaa aagctattcc acaatcatac
     3061 ggcacgatgt tttctgattg ggtatcaacg atcgtatgcg gaaatgcacg gctgatcgta
     3121 aacgtctgac gataaaagcc gccccatttg gtaaaggtga tcagttcaac aattgcgtta
     3181 ttccctgtta gctgcacagc cggctcgata ttattcatag cgcgagcttt gactttcccc
     3241 gcggcatcca gagggattcc aaaactttca taactgatgc tggc
//
LOCUS       DASTJP010000022         6240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_840066, whole genome shotgun sequence.
ACCESSION   DASTJP010000022 DASTJP010000000
VERSION     DASTJP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840066"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1000)
                     /locus_tag="VFQ23_00620"
     CDS             complement(32..1000)
                     /locus_tag="VFQ23_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905100.1"
                     /translation="MNNGPVSNSNQPSTTAPVSWTDTPTAAVTSEVTLFAVSTLIHEV
                     SDSRTRIKKSVGDFAEILVESTPVIHSISRRSDGSVAGTSSLPWEFQNISEMRVCFSL
                     DVPCQLTDEWIPFELSPNVGLFGAASIQKFKIQVDWVGPRTLWVVTQFRDNLGNPVLS
                     LSDSYKDPEVIAQRSLEIIGIHNEATPIEAQPPVVQTAVAETKTAFPLTGSVQLGGGG
                     CCIGGTDGDTIEVQVSFSATSPFGDVKEMRVRTGGICFSESEMTGTSWEPFVSSKTYP
                     VYVALNWIGFYVSAQYRDNYGNLSPVYCDDISVEGFPSTLTNTPSS"
     gene            complement(1390..2544)
                     /locus_tag="VFQ23_00625"
     CDS             complement(1390..2544)
                     /locus_tag="VFQ23_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905101.1"
                     /translation="MNIFKKLLVLILYLFIQACHPSTSKQATVLPISTTPFATKDIQT
                     SQTSTPVYLSSPTPSYSLSPLPFLSEDLNTIGSSNISSLVKLIEFQSQNSYAISIHWS
                     TNSKQIHILRSDGAIEIWDTQKGNLVKVFEPAKKNSNGVGWYPTKNLVAYSDRITNEI
                     SVWDLTNNRYLFDLGINTFPFITAQLVTWSADNSKIVSGGWDNTLRVWDASSGDELKV
                     IQSDSPWFESVAWAPSEDLIAVGHKDGLVYVINTSNWKTINTLFGPNSGHEGGAPVVS
                     WSPDGTKIASGGRDEAKAFVWNSSGAQLYQLNNQPLSVFGLGWSADSKMIATSGEGWD
                     VVITDSSTGSELFRIGDHKPNTYCVAWSPDGKLLATSTSEGKIRIWGIRP"
     gene            complement(2657..3466)
                     /locus_tag="VFQ23_00630"
     CDS             complement(2657..3466)
                     /locus_tag="VFQ23_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9905102.1"
                     /translation="MTADSQNKKDTLQGVFTRSASSYGQIRYFPIFGEWLVETAQIPQ
                     GATVLDVACGRGAVLFPAAERVGPGGRVIGIDLAEGMARETQMEIERRGLKHAEARQM
                     DAENLTFPDSTFDFVLCGFSMQFFPHLEQALTEFKRVLRPSGHVAVTTWGADDERWDW
                     YEDLRTDYGATISLGSQSLDKPDEIQDWFSKAGFVDIQIHTKEIDLAYRDEEEWWNVL
                     WSISGRAGLEKIGAEKLEQLKAEAFEKMQAQKEADGFHHRLEAFCTFAARE"
     gene            complement(3547..3951)
                     /locus_tag="VFQ23_00635"
     CDS             complement(3547..3951)
                     /locus_tag="VFQ23_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905103.1"
                     /translation="MAESPLAKKMKLRSGLKAAVIHAPESYVDTLKHDTALSPTLRGK
                     FDWIQIFVRTRTELDDLAPKAAKALRPESILWISFPKGTSKIQSDLTRDKGWDNVKEL
                     GLKWVNLVSVNDTWSAFALRPYKEGEEKKSWW"
     gene            complement(3970..4215)
                     /locus_tag="VFQ23_00640"
     CDS             complement(3970..4215)
                     /locus_tag="VFQ23_00640"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET9905104.1"
                     /translation="MCRSIKQLRNAEIPATEEEVRAAALQFVRKVSGYRKPSQANQAV
                     FDQAVKDVTQVTQRLLDQLKVHSHLSLSKASDHSAMN"
     gene            complement(4231..5559)
                     /locus_tag="VFQ23_00645"
     CDS             complement(4231..5559)
                     /locus_tag="VFQ23_00645"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9905105.1"
                     /translation="MLESLSPQANKLRFPDDIEQQFREEYRVTTLSATRIAMALGIVL
                     FSLFGILDIYAVPISKNFVWFIRYAIVDPLLIFIFIATYLPRLQRYIQPLVSIGIAVT
                     GLGIVSIITVAREAEFGSKFYFGGLLLVSMWAYGLARLRFQYAVLANMIILIGYEAAS
                     IVIKQLLASETGIVIFAIHNFFFLGANVIGMFASYNLERYTRLDFLQKYTIQAQRDQS
                     DKLLFNVLPARIAEKLKKSNETIAEQFSSASVLFADIVNFTPISARFDPLEVVEMLNE
                     LFSHFDELVDKHSVEKIQVAGDGYMVAAGVPAPRKNHAVVLAHLALEMMEYVKAQEFL
                     GGRHSIEIRIGINSGQVIGGVIGRKKFVYALWGDMVNTASRMESHGASGKIQITRATY
                     ELIKDHFECEYMGEIPVKGKGNMEAWHLIREKEKHRTTRRYIKSLNPEIR"
     gene            complement(5591..>6240)
                     /locus_tag="VFQ23_00650"
     CDS             complement(5591..>6240)
                     /locus_tag="VFQ23_00650"
                     /inference="COORDINATES: protein motif:HMM:NF020680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HET9905106.1"
                     /translation="VALFLRFQEANDNAANALLKTLEEAPSYVVLILTADNPEQLLPT
                     IVSRCEVLRLRPLPVEVVQRELERKGLETQSAKLIAHISGGRYGYALRLLEDSALLEK
                     REEHLEDLQRLIAASRVQKFGYADKLSKDKDVMRRTLLIWLSYWRDVMLRTAQAATPL
                     INVDRNVEIEDLAGRMNLSSTHTVVRRLEKALEQLERNVNPRLLAEVLLLDLPQL"
BASE COUNT         1574 a         1512 c         1424 g         1730 t
ORIGIN      
        1 tgctcccgag tgaaatgaga tttgttttac tttacgatga aggcgtgttt gttaacgtgg
       61 acggaaaccc ttccacgcta atgtcgtcgc agtagacagg cgatagattt ccgtagttat
      121 cccgatactg cgcgcttacg taaaaaccga tccagttgag agcaacatac actgggtagg
      181 ttttggatga cacaaatggc tcccagcttg tgccagtcat ctcggattct gagaagcaaa
      241 ttccaccagt tcgcacccgc atttctttca cgtctccaaa aggactcgtc gctgaaaagc
      301 tcacttgtac ttcaatggta tccccatcag taccgcctat gcagcacccg ccgccgccca
      361 gctgcacgga cccggtcaga ggaaaggctg tctttgtctc tgcaactgca gtttgaacta
      421 ccggtggctg cgcttcaatt ggcgtcgctt cgttatgtat gccgataatc tcaagtgatc
      481 tctgggcgat gacttccggg tctttgtagg agtcactcag ggaaaggacc gggtttccaa
      541 gattgtcccg aaactgcgta accacccaga gtgtgcgtgg tccgacccag tcaacctgga
      601 ttttgaattt ttggatggat gctgctccaa agagacccac gttgggggat aattcgaagg
      661 gaatccattc atccgttaac tggcagggca cgtctaaaga gaaacatacc cgcatttccg
      721 agatattttg aaactcccag ggtagactcg atgtccctgc gacacttcca tcacttctgc
      781 gtgagatact gtgaattacc ggcgtcgact ccacaagaat ctctgcaaag tcgcccacac
      841 ttttcttgat gcgtgtacgg ctatcactca cctcatgaat gagagtagat acagcgaata
      901 atgtcacttc ggatgtgacg gctgctgtcg gggtatctgt ccatgatacc ggcgcagtcg
      961 tcgaaggctg gttagaattt gaaaccggcc cattattcac aaaagcagta caggccgcca
     1021 aaataaggca cagaaatata agtgaggcgc gtttcaagtt atggactttc ctttagatct
     1081 aaatattaaa cgctgttgta ttgttaggag ttcccacttg aaactgacca gaagaagtta
     1141 tggagtcgga ttttctgcta ccctaattga tcaggaagaa tcaaaccgct taaccagcca
     1201 atgaaaggct cagaataata catattttgt gcaaatcttt ttctcctttt gaattcatag
     1261 cgtatacacc gttatgtcgt gaggaataat gggcaatcct gaggatttag gggaagttga
     1321 tttaggcaat ttattaggaa gaaatcgcag tagattgcaa tcaaatgctg cacttttcat
     1381 gatgttgttc taaggacgaa ttccccagat tctgattttt ccttcagagg ttgaagtagc
     1441 caataatttt ccatctggtg accaagccac acaataggtg tttggcttat gatctcctat
     1501 tctaaataat tcagaacctg tagaactatc tgtaataacc acatcccaac cttctccact
     1561 agtagcgatc atcttgctgt cggcggacca acccaaaccg aagactgaca atggttgatt
     1621 gttcaattga tacaactgag ccccgcttga attccaaacg aatgctttcg cttcatctcg
     1681 acctccagaa gcaattttgg ttccgtccgg cgaccaagaa acaaccggag cacccccttc
     1741 atgtccacta tttggaccaa aaagtgtatt tatagttttc caattactcg tattaataac
     1801 gtaaaccaaa ccatctttgt gtcccaccgc aattaaatct tcagatggag cccatgccac
     1861 gctttcaaac cacggcgaat cactttgtat gactttaagt tcatccccac tagatgcgtc
     1921 ccaaactctc aaagtattat cccagccacc tgaaactatc ttggaattgt ctgctgacca
     1981 agtaacaagt tgtgctgtta tgaatggaaa agtgtttatt cctagatcaa ataaatatct
     2041 gttatttgtt aggtcccata cagagatttc gtttgtaatt ctatctgagt atgcaaccag
     2101 atttttcgta ggataccaac ccacaccatt tgagttcttt ttcgcgggtt caaagacttt
     2161 tacgagattt cctttttggg tatcccagat ctcgattgcc ccgtcacttc ttaatatatg
     2221 tatttgtttg ctatttgtgg accagtgaat agaaatagca taactatttt gagattgaaa
     2281 ttcaattaac ttaaccaatg aagaaatgtt tgaagaaccg attgtattca ggtcttccga
     2341 taaaaatggt aaaggcgaaa gtgaataaga gggcgtaggc gacgataagt ataccggggt
     2401 agaggtttgt gatgtttgga tatctttagt tgcgaatggt gttgtagata tcggtaggac
     2461 tgtggcttgt ttggatgttg acggatggca tgcctgaata aataaatata agatcaaaac
     2521 caataatttc ttaaatatgt tcatgtgtga aattttgaat gagatggata tatcgattaa
     2581 ttatgagttt acttatgata ttttttctgc ttctaaaaga cgtcaagact aacctttggc
     2641 aacctccgtt ttatggttat tccctcgctg caaatgtgca aaacgcttcc agccgatggt
     2701 gaaatccatc cgcctctttc tgtgcctgca ttttctcaaa tgcttctgct ttgagttgct
     2761 ctaatttctc ggcgccgatt ttttccaacc cggcgcgccc ggagatggac cataggacat
     2821 tccaccattc ttcctcatcg cgataggcta gatcaatttc ttttgtgtga atttgtatgt
     2881 cgacaaagcc agctttagaa aaccaatctt gaatttcatc tggtttatcc aggctttgtg
     2941 atcctaaact gatcgtagcg ccatagtcag tgcgtagatc ttcgtaccaa tcccatcgtt
     3001 catcgtccgc accccaggtg gtgactgcga catgcccact cggtctaagc acacgtttga
     3061 attcagttaa ggcctgctca aggtgaggaa aaaactgcat ggaaaagcca cacaggacaa
     3121 agtcgaaggt ggaatcgggg aatgtcagat tctcagcgtc catctggcgc gcttcggcgt
     3181 gtttgagtcc gcgccgttca atttccatct gcgtctcacg cgccatgccc tctgccaggt
     3241 caatgccaat gactcgtcca ccgggaccga cgcgttccgc agcggggaat agcaccgcgc
     3301 cgcgtccaca ggcgacatcc aggaccgtgg cgccttgggg gatttgggca gtttccacca
     3361 gccattcccc aaagattggg aagtaccgga tttgcccgta actggacgcc gagcgtgtga
     3421 aaacgccttg taaggtatct tttttgtttt gtgaatccgc ggtcacatca tgctcctaac
     3481 gtgacagtca cctttgcgaa gcactccctg gcgaagcgca gggagcgagg tgactgtcac
     3541 gtttatttac caccaggatt ttttctcttc gccttctttg tagggtctca aggcaaatgc
     3601 agaccatgtg tcattgactg aaacgagatt gacccatttc aaaccaagtt ctttcacgtt
     3661 gtcccagcct ttgtcgcgag tcagatcgct ttggattttg gacgtccctt tcgggaagga
     3721 aatccacaag atggattcgg gtcgcaacgc cttcgcggct ttcggcgcga ggtcatccaa
     3781 ttccgttctg gtccggacga agatttggat ccaatcgaat ttccctctga gcgtgggcga
     3841 taacgcggtg tcgtgcttca acgtgtctac atagctttct ggagcgtgga tgacagccgc
     3901 tttcaaccca gacctgagct tcatcttttt tgcgagcggg gattctgcca tgatcgattc
     3961 ctttacggat caattcattg cactatgatc gctggctttt gagagactga ggtgggaatg
     4021 tactttaagc tgatccaaca atctctgagt tacctgagtc acgtccttta ctgcctgatc
     4081 gaaaacggcc tgattcgctt gtgacggctt ccggtacccg ctcacttttc tcacgaactg
     4141 aagcgccgca gcgcgcacct cttcttctgt ggcggggatt tctgcatttc gtagctgttt
     4201 gatgcttctg cacatgttgt actcctcgtg ctatcgtatt tcagggttga gtgattttat
     4261 atatctgcgc gtcgttcggt gtttttcttt ttcgcggatc aaatgccagg cttccatatt
     4321 gcctttgcct ttaactggga tctcacccat atactcacat tcgaaatggt ctttgatcag
     4381 ttcgtaggtt gcgcgcgtga tctggatttt tccactggca ccatggcttt ccatgcggct
     4441 ggcggtgttg accatatcgc cccataaggc gtagacgaat tttttgcgtc cgataacccc
     4501 accgatgact tgccccgaat ttattccaat gcggatttct atagagtgac ggccacccag
     4561 aaattcctgc gctttgacgt attccatcat ttcgagcgcc aggtgcgcaa ggacaaccgc
     4621 atggtttttc cgcggagctg gcacacctgc ggctaccatg tatccgtcgc ctgccacctg
     4681 gattttttcc acgctgtgtt tatccacaag ttcatcgaag tgcgagaata actcgtttag
     4741 catctccacc acttcaagcg gatcaaagcg cgccgaaatt ggtgtgaagt taacgatatc
     4801 tgcaaagaga acgctcgcag aactgaattg ctccgcaatg gtctcattgc ttttctttag
     4861 cttttcggct atccgtgctg gcaaaacatt gaagagaagt ttatcggact ggtcgcgttg
     4921 cgcctggatg gtgtactttt gcaggaaatc caaacgggta tagcgttcca ggttatagct
     4981 tgcaaacata ccgatcacat ttgcgcccag aaagaaaaag ttatggatgg cgaagatcac
     5041 aatgcccgtt tcggaggcca ataactgttt gataacgata ctggcagctt catatccgat
     5101 cagaataatc atgtttgcca gtacggcgta ctggaaccgc agacgcgcca acccataggc
     5161 ccacatggaa accagcagca gaccaccaaa ataaaatttg cttccaaact ccgcttcccg
     5221 cgcgaccgtg ataatcgaaa caatccctaa gccggttaca gcaatgccaa tgctgaccaa
     5281 aggctgaata tatcgttgaa gccgtggaag gtaagttgca ataaaaatga agatcaaaag
     5341 gggatcgacg attgcgtagc gaatgaacca gacgaagttt ttggagattg gaacggcata
     5401 aatatccaaa atgccaaaca gggaaaacaa aacgatcccc aatgccatcg caatgcgcgt
     5461 ggcagagagc gtggtgacac gatattcttc ccgaaattgt tgttcaatat catcggggaa
     5521 acggagtttg tttgcttgag gagaaagcga ttcgagcata ggtttctcga cgtaaaaagt
     5581 cggcactaca ttacagttgt ggcaaatcca acaaaagtac ttcagccagc agtcgcgggt
     5641 tcacattcct ttcgagctgc tccagggctt tttcgagtct tcgtactacg gtatgtgtgg
     5701 atgaaagatt catccgcccg gccaaatctt caatttccac attgcggtct acattgatca
     5761 gcggagtcgc tgcttgcgca gttcgaagca tgacatcgcg ccagtaggag agccagatta
     5821 atagtgtgcg ccgcataacg tctttgtctt tcgacaattt atcggcatag ccaaatttct
     5881 gcacgcgcga ggcagcgatt aaacgttgaa ggtcttccaa atgttcctcg cgtttttcaa
     5941 gcaacgccga atcttcaagc aatcgaagcg cataaccata tcgcccaccg gagatatggg
     6001 caatcagctt tgcgctctga gtctccagcc ccttcctctc taactctctt tgcaccactt
     6061 caaccggcag tggtctcaac ctcagcactt cgcagcgaga aacaatcgta ggcagtagct
     6121 gctcagggtt atcggcagtt agtatcaaca cgacgtacga gggcgcttct tccagtgtct
     6181 tcaacagagc gtttgcagcg ttatcgtttg cttcctggaa tcttagaaag agcgccacgc
//
LOCUS       DASTJP010000023         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1051503, whole genome shotgun sequence.
ACCESSION   DASTJP010000023 DASTJP010000000
VERSION     DASTJP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051503"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1143..>2456)
                     /locus_tag="VFQ23_00655"
     CDS             complement(1143..>2456)
                     /locus_tag="VFQ23_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9905107.1"
                     /translation="PIQNWLRLIEAGTPAAELPSSVARLVLIWPLIVASLVAATGLLV
                     AIFFSILYDNQGNFVGIAIGGAVTTTIVYFGTDLIWRQQVPTFFPEGNLSVVPAFRLW
                     VRRRLLLAFILIGGLTPILLVILSEVRTRALLDAENPKAILDNLILVQLFILAVGLVA
                     GVITAVLVARAIVGPLQVLQEAMGRVEKNELNTRIVVTTNDELGYLSERFNSMTDGLR
                     QGERLRELFGLYVSSEVAQAAVETGAGLGGELVNCSVMFSDIRDFTTLSEQMQPRRLV
                     ELINRYMTAMVSVIVNHGGVVTRFGGDSILAVFGTPLNPMTDHADRAVRTAIEMRQAL
                     AAFNQEETVARLPILESGIGIASGPVIAGNVGGKERIEYTVMGDAVNLAARLEDKTKD
                     TGFPILVSDETYRALNEVSDVGVRSLTDVQIKGKRDRVTVYALSD"
BASE COUNT          620 a          674 c          573 g          589 t
ORIGIN      
        1 catcttccac caccgcgttc agctgttcaa cggtcattcc atccaggtcg cggccggtga
       61 gcgttttccc tttttcggca atgcctaaat ccccggcgat gatcttcgct gccagacgct
      121 gccacaccgg tgttttcgcc atggatgcca gtccttgctc aatttcagtc tactcattca
      181 gattcttgtt gtcatctgtc aaatgtcacg acccacaact cttgtacttt aaggcaacac
      241 cctcagagaa agtgcgttcc agcgccgcaa ttagggttta tagtccgcat gatgactaat
      301 tgaaatgaat tttcccggga ccgcaggttg aaacaatttt gtgcagttca cagaatgtct
      361 ctttttatcc tccgaagctg cacagccacg cttcaagccg aaacatcaat acccaaggct
      421 gctgcacaat gactggcatc tggccccacg ccctcctcac gagaccttgg cccgcttcat
      481 ccagcttgca gaaactcaag ttcttttttt atggcccact aaaagtgtgc acgatctgtc
      541 tttcgttagg ttatgtcaaa agactcagat ttgcgttgac gaatcaaaag tctcgcgcac
      601 attgatgcat ggagtaagtg ttgctataat cagaattatc caatttactg agcgcccaag
      661 tagttacgcg tcgcgagccc caatcccgtc aggaccggat ttataggcaa cttgatgaga
      721 tttcatcaag ttgttttgtt ttaataccta ggtgagtcaa aatcatcgtt cccctatatt
      781 gtgtctatta aatctatacc acaaaattat ggacatcgtc catcacttta gatgccgatc
      841 aatagaattt aatgccttct ccaaattaaa tgaagtcgca aaatctgatt ttgcgcacta
      901 tttatcaatc ctatgatcgc gaaatgctgg gatttagggt atcctagtca gaaatatgtt
      961 cgcacaatgg ttacagacag ttgaaaaagt aatactaacg acaggttaga tttggaaaac
     1021 aacatacaac cctgtttata tggataatcg agcatacctc gaaatcgctt gaatgaacct
     1081 gaaagtatgc cccatgcgat tatatattca aataggtcag gttcataagc caccgtacca
     1141 ggttaatcgg aaagggcata tacggttacc ctgtctcgct ttcccttgat ctgaacgtcg
     1201 gtcagcgacc tgacgcccac gtccgaaact tcgtttagcg cccgataggt ttcgtcgcta
     1261 acaagaatgg gaaagccggt atccttggtc ttgtcctcca gcctggcggc cagattgacg
     1321 gcatccccca tcaccgtgta ttcgatacgc tccttgccgc cgacgttgcc ggcaatgacc
     1381 ggcccactgg caataccaat ccctgactcg aggattggta ggcgggcgac cgtttcctcc
     1441 tgattgaaag ctgccaatgc ctggcgcatc tcaatggccg tcctcactgc tcgatctgca
     1501 tgatcggtca tgggattgag gggcgtgcca aataccgcca ggatagagtc tcctccaaag
     1561 cgagtgacca cacccccatg attgacgatg acagacacca tggcggtcat gtagcggtta
     1621 atcagttcaa ccagccgtct gggctgcatt tgttcgctga gagttgtgaa gtcacgaata
     1681 tccgagaaca tcaccgagca gttgactagc tctccaccta gaccggcccc ggtttccacg
     1741 gccgcttgag ccacttcaga actgacgtaa aggccaaata actcccgcaa acgctctccc
     1801 tgtcgcaacc catctgtcat cgagttaaac cgctcgctca ggtatcccaa ctcgtcgttg
     1861 gtggtcacca caatcctggt attaagctcg ttcttttcca cgcgacccat cgcctcctgc
     1921 aaaacctgca gtggtcccac aatggcgcgg gcgacgagta cagccgtgat cacaccggcg
     1981 accaggccga ctgcgaggat gaataactgc acaaggatga gattgtccag aattgccttg
     2041 gggttctcgg catcaagcaa ggcgcgggtg cgtacctccg acagtatgac gagcaagatt
     2101 ggcgtaagcc cgccaatcag gatgaaggcc agcaacaagc gcctccgtac ccatagtcga
     2161 aaggcgggaa caacgctcag attcccttcg gggaaaaagg tgggtacctg ttgacgccag
     2221 atcagatcgg tgccaaagta aacgatggtc gtcgtgacgg cgccgcctat ggctatgccg
     2281 acgaagttgc cctgattatc gtacaagatg gagaaaaata tggccaccag cagcccggtc
     2341 gcggccacga gcgaagcgac aatgaggggc caaatcagta ccaaacgggc aacgctcgac
     2401 ggcaattctg cagcgggcgt gccagcctca attaaccgca gccaattctg gatggg
//
LOCUS       DASTJP010000024         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_839295, whole genome shotgun sequence.
ACCESSION   DASTJP010000024 DASTJP010000000
VERSION     DASTJP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839295"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFQ23_00660"
     CDS             <1..463
                     /locus_tag="VFQ23_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905108.1"
                     /translation="APLLEVDIHEGLENTLVIMQHKLKQGVTIKREYSSDLPRIEAYA
                     SELNQVWTNIIDNAIDALNGRGEITLRTYAEDNKAVVEIVDNGPGIPEEIQARVYEPF
                     FTTKPPGKGTGLGLHISHDIVANRHHGLLLVESKPGETKFKIILPKQIKGE"
     gene            466..888
                     /locus_tag="VFQ23_00665"
     CDS             466..888
                     /locus_tag="VFQ23_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9905109.1"
                     /translation="MNNDQMMKEILLSSKTIASVGLSGNQQKESFWIASYLKEQGYRV
                     IPVNPTATEILGEKAYPDLSSVPEKVDVVQVFRKPEDVPPVVDEAIKIGAKFVWMQEG
                     IVHEEAAQKARDAGLQVVMDACMRVTHRRLVGPKPIGL"
     gene            complement(967..1581)
                     /locus_tag="VFQ23_00670"
     CDS             complement(967..1581)
                     /locus_tag="VFQ23_00670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9905110.1"
                     /translation="METIRGKMSQNLGLIELSERFKGYNQIALDLGTGDGRFVRCMAE
                     KQPDQFFIGIDACRENLRVNSRTRLPNALFVIASAQALPLELNGLASQIKINFPWGSL
                     LKGLLINDCCLLHGLFAITHPCAELEIHLNGEALLTAGWELEAGTDQIENNLSAAGWK
                     IKSRSWLDSQVLRAIPTTWAKRLAFGRDPRAIRLTLQKDEEKLV"
     gene            complement(1888..2499)
                     /locus_tag="VFQ23_00675"
     CDS             complement(1888..2499)
                     /locus_tag="VFQ23_00675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9905111.1"
                     /translation="MKWVASILVYPAVFIGLFWLHHAWGALLGFHLAILISLFYAKPN
                     VPIRILFKSSSAKWILLSILLCGSSGVSLYFLWPLFGIPNNISEQTLSLGLNSHSWFF
                     FIAYFALVNPFLEEYFWRGYLGDTTYNFSMYDLIFAGYHVLIVIGRAHWLSITFSIAV
                     LTLGGWFFRQVAREDRGLLAPVLGHMSADFMILAAIFLRTQNI"
     gene            complement(2496..>2948)
                     /locus_tag="VFQ23_00680"
     CDS             complement(2496..>2948)
                     /locus_tag="VFQ23_00680"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905112.1"
                     /translation="FTKGKPIEIFKVGKPFTIVIGDTGVSAPTKESVADVRKLWTENP
                     KRFEVIFDEVADISFAARRVIEEGWVDMLGTLMNENHAYLQEMTVSSPELDRLVSAAR
                     TAGASGAKLSGGGRGGNMIALVEEHKAQNVAEALMGAGAKRTIITKLS"
BASE COUNT          812 a          705 c          721 g          710 t
ORIGIN      
        1 ggcacccctg cttgaggtgg atattcatga agggctggaa aatacgctcg taatcatgca
       61 acacaaattg aagcagggcg tgaccataaa acgtgaatat tcttctgacc tgccgcgcat
      121 cgaagcctat gccagcgaat tgaatcaagt atggacgaat atcattgaca atgcaattga
      181 cgcgctgaac ggcagaggcg aaataactct gcgcacatat gcggaagata ataaggccgt
      241 ggtggaaatt gtggataacg ggccggggat cccggaagag attcaggccc gcgtgtatga
      301 gccgttcttt accaccaagc cgccgggcaa aggaacaggg ctggggttgc acatctcgca
      361 tgacattgtt gcaaatcgtc atcatggact attgcttgtc gaatcaaaac ccggggagac
      421 gaagtttaaa attatcctgc caaagcaaat caaaggagaa taaagatgaa taacgatcaa
      481 atgatgaagg aaatcctttt gtcttcaaag acgatcgcga gcgtcggctt atctgggaat
      541 caacaaaagg aaagtttttg gatcgcctcc tatttgaagg agcaggggta tcgagtgatc
      601 cctgtaaacc cgacggcaac tgaaatattg ggtgagaagg cgtatcccga tctatcgtcc
      661 gtgccagaaa aggtcgacgt cgttcaagtc tttcgtaaac ctgaagatgt gccgcccgtt
      721 gtggatgaag cgatcaagat cggagcaaag tttgtctgga tgcaggaggg aatcgtccat
      781 gaagaagccg cgcagaaagc cagggacgcg ggattgcagg tcgtgatgga tgcctgcatg
      841 cgtgtcacgc accgcaggct ggttgggcca aaaccaattg gattgtaaat atgagaccct
      901 aaaggtttaa ttatcccggg aatatgttcc tcggtataag aaaaacttta gggcctattc
      961 tttttgttaa actaacttct cctcgtcttt ttgaagagtt aatcttatcg ctcgcggatc
     1021 acgaccaaag gcaaggcgtt tcgcccaggt cgttggaatt gcgcgcagta cctgggaatc
     1081 cagccatgat cgtgatttga ttttccagcc agctgcactc aagttgttct cgatttgatc
     1141 tgtacccgct tccagctccc aacctgctgt aagcagtgcc tctccgttca ggtggatttc
     1201 cagctccgcg cagggatgcg tgatggcgaa aagcccatgg agcaaacagc aatcattaat
     1261 cagtaagcct ttcagcaaac ttccccatgg gaagttaatt ttgatttgag aagccagtcc
     1321 attcaattcc aacggtaacg cttgcgcact ggcgatcaca aatagcgcat tcggaagcct
     1381 ggtgcgcgag ttgacgcgta gattctcacg gcatgcatca atgccgataa agaactgatc
     1441 tggttgtttt tctgccatgc agcgcacgaa gcgcccatcc cctgtaccca ggtccagcgc
     1501 gatttgattg tagcccttga atcgttccga taattcgatc aaacctaaat tttgggacat
     1561 tttgccccga attgtttcca tcagaaacct ccattctgtc gtgtgaattg gtaaagacag
     1621 aatggagcgg ggcggaccac gtgcacgatg ggcagccgcc aacgcgaaga gacgccatgc
     1681 tacgcgtcag gcgccgtcta gatcatcggg tgtaggtttt ttacgggtta gggaaaatac
     1741 aaaacaggcc catcgggcct tttgaacatt gtatgttctt agagagacgg tctagcaggg
     1801 gggtcgagcg aagaacctgt tcatatagca aattatagca ttagattcaa aacctggaaa
     1861 ttagcgtctt gccgtatttt ctccagctca aatattctgc gtcctcaaaa atattgcagc
     1921 caggatcatg aaatctgccg acatatgccc caacaccgga gcgagcagac cccgatcttc
     1981 acgtgcaacc tgcctgaaaa accagcctcc caatgtaagc acggcaatgg aaaacgtgat
     2041 ggaaagccag tgcgcccttc caatcacaat aagcacgtgg taacctgcaa aaattaaatc
     2101 gtacatggaa aagttatagg tggtgtctcc gagatagccg cgccagaaat attcctcgag
     2161 gaatgggttg accaacgcga aataggcaat gaaaaaaaac cagctgtgag aatttaatcc
     2221 taaggaaagg gtttgttcag aaatattgtt tggaatccca aatagaggcc aaagaaaata
     2281 taaactaacc ccgctgcttc cacataacag aatacttaag agaatccatt ttgcactcga
     2341 actcttaaag agaatcctga taggtacgtt cggctttgca tagaataatg aaatgaggat
     2401 cgcaaggtgg aaccccagca gtgcccccca cgcatggtgt aaccagaata agccaatgaa
     2461 cactgcggga tatacaagaa tcgacgcgac ccatttcatg atagcttagt aatgatcgtt
     2521 cgttttgctc ctgcgcccat cagggcttct gctacattct gtgccttgtg ttcttcaacc
     2581 agcgcaatca tattgccgcc gcgaccgcct ccgctgagtt ttgcccctga tgcaccggcc
     2641 gttcgcgcgg ctgagacgag ccgatccagc tcgggtgaag atacggtcat ttcctgtaaa
     2701 tacgcgtggt tttcattcat tagcgtgcca agcatatcca cccagccctc ttcgatcact
     2761 cgacgggcag cgaaggaaat atctgctact tcatcgaaaa taacctcaaa gcgttttggg
     2821 ttctctgtcc acaattttcg aacatcagct accgattcct tcgtcggtgc agaaactcct
     2881 gtatcgccga tgacaatggt gaacggcttt cccactttga atatttcaat gggttttcct
     2941 tttgtgaa
//
LOCUS       DASTJP010000025         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_100778, whole genome shotgun sequence.
ACCESSION   DASTJP010000025 DASTJP010000000
VERSION     DASTJP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100778"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..836)
                     /locus_tag="VFQ23_00685"
     CDS             complement(264..836)
                     /locus_tag="VFQ23_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905113.1"
                     /translation="MKNLRISVFFTLSTIIVLVITGCAPASVEPLATSISSQPENTME
                     ENAPLPTNVPHTVAEVEELAGFDVKEPAYLPTGVSFDYATYQEAPFPNVTLHFKLVHE
                     TYGDMGAFFQIVQEPQAEAMPNPAACGATGNDCEVLQVGDVAVNYRLTSPTESLMWSS
                     NGFSFQLLRTAGEPEKIYKDELLKVVESMS"
     gene            complement(1050..1259)
                     /locus_tag="VFQ23_00690"
     CDS             complement(1050..1259)
                     /locus_tag="VFQ23_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905114.1"
                     /translation="MKLVYAVSQWTNAKRPAARHLDNGEGKPLCGGNGRQPLLWATEK
                     GEPTCKICIDLALMLEGETDEVNET"
     gene            1362..2336
                     /locus_tag="VFQ23_00695"
     CDS             1362..2336
                     /locus_tag="VFQ23_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905115.1"
                     /translation="MSSITVPANTNEQTNVRALNVIRDLSAVADLIELCFSPTMDNEG
                     QRYLSDMRRASRDDGFLHWASRMTETTSLPLTGYVWEQDGRIVGNASLIPFRHKGSRV
                     YLIANVAVHPDHRRQGIAQVLTQRAMKHAQAKKASAVWLHVRDDNLGAIRLYEKLGFQ
                     EISRRTTWYARTDSYPPKVDTDIQIVPRHARFWPLQQDWLRRLYPDALSWYHSWNFNS
                     LRPGLMNWMYLLFVDYNIRQWAAVRGDELLGTLSWMPHGGRAESLYAAINPAMDGDEE
                     ALLQLLINARRSLSIHSKLTLDFPAGEMTDAIMEAGFRPRRTLIWMSA"
     gene            2430..2807
                     /locus_tag="VFQ23_00700"
     CDS             2430..2807
                     /locus_tag="VFQ23_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9905116.1"
                     /translation="MTKFILRWAINAIALYLAVQILPGIGLRGDLVSILWLALIFGLV
                     NALFRPLIQILTCGLIILTLGLFTLVINTFLFWLTSVIGNSFGVGLTISDPVWWNAFL
                     GGLVVSIVSVVMTLILKDELKGK"
BASE COUNT          731 a          754 c          736 g          820 t
ORIGIN      
        1 ttttgctcat ccagcctgga cgacacgtga gtattttcca ccccagaaag ttccagcaag
       61 cctatgatcc caccgaaatg agtattttgc cattcatcgg ttgctaaaat tgaaagcata
      121 tctgtctcca ggtcataaag aaaatgttac gtcaggattt ttttaaatga aactagcgca
      181 tcataatgcg cagattataa aacaacaccg cgttgccaga ggcgcaacgc ggtttggagt
      241 aaagctagaa agaagatgct ttactagctc atactttcaa cgacctttag taattcatct
      301 ttgtaaattt tctctggttc tcccgcagtt cttaaaagtt gaaatgaaaa gccgttcgaa
      361 ctccacatca gcgattctgt ggggctagtc aaccggtagt tgacagccac atcgccaact
      421 tgtaatactt cacaatcatt gccagttgcg ccacaggcgg ctgggtttgg cattgcttct
      481 gcctggggct cctgtacgat ctggaaaaag gcccccatat ctccataggt ctcgtggaca
      541 agcttgaaat gtagagtcac atttgggaac ggtgcttcct gataggtggc ataatcgaac
      601 gatacaccgg ttggcaggta agcaggttct ttcacatcaa agcctgctaa ttcttcaact
      661 tcggctacag tatggggaac gttcgttggc aaaggtgcgt tttcttccat cgtattttca
      721 ggttgagatg aaatcgatgt tgcaagaggt tcaacggagg cgggtgcgca tccagttatt
      781 accaaaacga tgatggtgct taacgtaaaa aatactgaaa tcctcaaatt tttcatggtt
      841 ttatctcctt ttcagctaaa tacgcagggg gaatggtgaa ctatagctta accagacctg
      901 gcgccctgta aaacgtcacg ggggaggatt tctttaaaga ttttagataa actatgccct
      961 cgcagatggt gcaataccac aatacgcccg gatggtttat aaaaagtcaa ggaactgttt
     1021 tgggtagttc tcccttgaat catagacgat catgtttcgt taacttcatc tgtttcaccc
     1081 tccagcatca aggcaaggtc tatacaaatc ttacaggtag gttccccttt ttctgtagcc
     1141 cacaataatg gctggcgtcc attaccgccg cacagtggtt tgccttctcc gttgtcaaga
     1201 tgacgagcgg caggacgttt ggcattcgtc cattggctga ctgcataaac gagtttcata
     1261 gctctccttc ctcgattatt tgtagaacaa atccgttgat tttgcgggat tatacgcctc
     1321 ccgccttcca aattaagaca acagtactat aatttgcctc aatgagttcc atcaccgttc
     1381 ctgccaacac caacgagcaa acgaacgtac gagctcttaa cgtgattcgc gatctctctg
     1441 cggtggcaga cttgatcgag ttgtgctttt ctcccacaat ggataacgaa ggtcagcgct
     1501 acctctcgga tatgcgccgc gccagccggg atgacggctt tttgcattgg gcctcgcgca
     1561 tgacggagac cacctccctg ccgctgacgg gttatgtgtg ggagcaggat ggacgcatcg
     1621 tcggcaacgc aagtttgatt ccatttcgcc ataaaggcag ccgagtctac ctgattgcga
     1681 atgtggcagt acaccctgac catagaagac agggcatcgc gcaggtcctg actcagcgcg
     1741 cgatgaagca tgctcaagcg aagaaagcca gcgcagtctg gttgcacgtg cgggatgaca
     1801 atctcggcgc gatcaggctt tatgaaaaat tgggatttca ggagatcagc cgccgtacta
     1861 cctggtatgc gcgcaccgat tcctaccctc ccaaagttga tacagatatc cagatcgttc
     1921 cacgtcacgc acgcttctgg ccgcttcaac aggattggct tcgccgcctc taccccgatg
     1981 ctttgagctg gtaccattcc tggaacttta actctctcag acccggcctc atgaactgga
     2041 tgtatctttt atttgtggat tacaacatca gacaatgggc agcggtccgc ggggatgagt
     2101 tgttggggac cctttcctgg atgccgcatg gaggccgagc agagtcgctc tacgccgcga
     2161 taaaccctgc catggacggt gacgaggaag ccctcctgca attgctgatc aacgcacgcc
     2221 gctcgctatc aatacattcc aaactcactt tggattttcc cgccggtgag atgaccgatg
     2281 caattatgga agcaggattc agaccgcgcc gcacactgat ctggatgagc gcttaagcgc
     2341 gacgatcggg cttttccctc gtatgaatag gtggcagtca atcgctttgc gatattgact
     2401 gtcacctatc gttttgaaga ggagaaccca tgacaaaatt tattcttcgt tgggccatta
     2461 atgcgattgc attgtatctg gcggttcaaa ttctgccggg catcgggctg agaggtgatc
     2521 tggtttcgat cctgtggctg gccctgatct ttgggctggt caatgcgcta tttcggccgc
     2581 tcattcaaat ccttacctgc ggattgatta ttctgactct cggcttgttc accctggtga
     2641 tcaatacctt cctgttttgg ctgacaagcg tgatcggtaa ttcctttggg gttgggttga
     2701 ccatcagtga ccctgtctgg tggaacgcct tccttggtgg attggtggtc agtattgtga
     2761 gcgttgtaat gactctgatt ctgaaagatg agttgaaagg gaagtaaaag caatacataa
     2821 ggcgtgaaaa acggcttgct ctggagcaag ccgttttttt tattgcaaca tcagcattac
     2881 aaatttccta ctgctggtaa cccgccggcg gacacggcca tctttactcc acgcacgatc
     2941 gcttgtgcca tgacctctgc tgcgaacgca ccaatcgttg aaatatcggc cttcctgttt
     3001 cccgttgata gcgcgaagat ggtatcacca tccagcatgg t
//
LOCUS       DASTJP010000026         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_205347, whole genome shotgun sequence.
ACCESSION   DASTJP010000026 DASTJP010000000
VERSION     DASTJP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205347"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1336
                     /locus_tag="VFQ23_00705"
     CDS             164..1336
                     /locus_tag="VFQ23_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9905117.1"
                     /translation="MMQVFRYATIVVITLAGLLLIWQFSVAIVLFLLSLAVAAALRPM
                     INSLMGSRVSKRLALAVVYSILVVLIVGTFFLVGARILDELQTASDDLLASYDRAKTQ
                     WPVRGTLFQQTLAEQLPPSANLYEALISGEGVPALQGVLGIAQDFFSILGNIAIVLIL
                     SLYWSADQLRFERLGLSLLPSEYHSRALHTWRSIEAGVGAYLRSEFVQSALAGLLLWA
                     IYLTLDIRYPALLAMWGAVVRLIPWFGALIAVLPALFVAIGSSPILGIVAAISTVVVL
                     FLLKEVIEPRVFHRHKYSSLLIVLFVIGLAEAFGFIGVILAPPLAVAVQIMFRELFPL
                     PASQFSQEVSDRVSNIRKRLLQLRRRIQRRTNRKNRQLINRIYYLVRRTSDYLQGY"
     gene            complement(1338..2639)
                     /locus_tag="VFQ23_00710"
     CDS             complement(1338..2639)
                     /locus_tag="VFQ23_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905118.1"
                     /translation="MLKFLIRISSFLTKELTEIFRQPKLILTMVLGPFLIMFLFGLAY
                     PDQNRALRTTYVSQDPSAFQEQMQIFTQAFSSTIVNQGVETDEALALSRLALNQTDLV
                     IVVPETPMETIESNQQAEFVIYHNEVDPFQIGYIQSAARIYVDEVNRLALQSVAEQGQ
                     EDTGSLQTDLESAMVKTQELRQALPPGDPNTARVDELESDLTKVHENLATFRSLSSGV
                     LVNPFTSDISGLSNIQFTTTGFFAPAVIVLLLQHLSITFASLSIVRESRSGIMELFRV
                     APITAFETLIGKYVSYLFFEIILAAVITALAVWALKIPLLGNWQDYALAVVIVLFTSL
                     GVGFLISLISETDTQAVQYSMLLLLASIFFSGFFLDLRLMWEPVKVLAWSLPATYGIQ
                     MLQDVMLRGGTVPPLIFQGIAAIGVALFLVDWFLLRRRMER"
     gene            complement(2632..3603)
                     /locus_tag="VFQ23_00715"
     CDS             complement(2632..3603)
                     /locus_tag="VFQ23_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9905119.1"
                     /translation="MNNQTNNDIAENVVETKDLTKTFGESVAVQSLDLYVPRGKIFGF
                     IGPSGCGKTTTVRLLTGFYEPTSGSVSVLGKEPTNFSQSDKEQIGYLVQGFVLYPELT
                     VWENLNFSASFYGVSLFRANRLNKLLDFVELAEHRNKLARDLSGGMKRRLSLAATMVH
                     NPELLFLDEPTAGIDPILRRKFWDYFKELQEEGHTLFVTTQYVGEAAYCDLVGVMYEG
                     RLLMVATPENLRKKAFGGEIVGIKTTEELRPEQRHQLENLPFVQSKVKVITDQDIEVI
                     VDEASTAMPLLLDWSKAQNLNIETIEEISPPFDDVFVRIIEKESAHA"
BASE COUNT          943 a          916 c          930 g         1047 t
ORIGIN      
        1 tggatgaaag caaacggctt ttgcaaattc ttgatggaga ctctacgaac aacaaagagc
       61 ccgttctgga tgacctgtct gaagaagacc ggttggagat ccaggcgatt acacacgaat
      121 tggagatggc tcttcaggaa ctaacaactg aggatgaaat catatgatgc aggtttttcg
      181 ttacgctacg attgttgtca tcacactagc cggcctgctc ttaatatggc agtttagcgt
      241 cgctattgta ttgtttctcc tctctctggc ggtggcggct gcgttgcggc caatgatcaa
      301 cagcctgatg ggaagtcgcg tttccaagcg ccttgcgctg gcggtcgtct attcgatttt
      361 agtcgttttg attgtgggaa catttttcct ggttggggca cgtattctgg atgaactgca
      421 aactgccagc gatgatctgc ttgccagtta cgaccgcgca aaaacacagt ggccggtgag
      481 gggaactctg tttcaacaaa cactggcgga acaacttcct ccctcggcaa atttatacga
      541 ggctctgatc agtggggaag gagttcccgc cctgcagggt gtcttgggaa tcgctcaaga
      601 ttttttctcg atccttggta atattgccat cgtcctcatt ctaagcttgt actggagtgc
      661 cgatcagctg cgattcgaac ggctgggact ttctttgctg ccatctgagt atcattcaag
      721 agctcttcat acctggcgat ccattgaggc gggtgtaggt gcatatctcc gcagcgaatt
      781 tgttcaaagt gcactggccg ggttattgct ctgggctatt tacttaacgc tggatataag
      841 gtatccggcg cttcttgcca tgtggggcgc tgtcgtcaga ttgatcccct ggtttggtgc
      901 gctgattgct gttctccccg ctctgttcgt tgcgatagga agctcaccta tattagggat
      961 tgttgctgca atttccaccg tggtcgtctt gttcctttta aaggaagtca ttgagccgcg
     1021 cgtgttccat cgccataaat acagttcact gttgatcgtg ttgtttgtta ttgggctggc
     1081 agaggcattt gggtttattg gcgtaattct tgcgccgccc ctggcagtgg ctgtccagat
     1141 catgtttcgg gaactgtttc cacttccagc ctcgcagttc tctcaggaag tatcagacag
     1201 agtttcgaat ataagaaaac gcctcctgca attgaggagg cggatacaac gtcgtacaaa
     1261 tcgcaagaac cggcaattga tcaaccgcat ctattatctg gtgcggcgaa cttccgatta
     1321 tttgcagggg tattagacta tctttccatt cttcgccgta gaagaaacca atcgaccagg
     1381 aacagagcaa ccccgatggc tgcgatgccc tgaaagatca gggggggcac ggttccacca
     1441 cgcagcataa catcttgtaa catctgaatg ccataggtgg ctggcagcga ccaggccagc
     1501 accttaaccg gttcccacat gagacgcagg tccagaaaga atccactaaa gaaaatgctt
     1561 gctagcagca acagcatgga gtattgaacg gcctgcgtat ccgtttctga aatcagcgag
     1621 atcaggaaac ccacgcccag ggaggtaaag agcacgatca caactgccag cgcataatcc
     1681 tgccagttgc ccaaaagagg aattttcaac gcccagacgg caagcgcagt gatcactgcg
     1741 gccagaatga tttcaaagaa aaggtaactg acatattttc cgatcaacgt ttcaaatgct
     1801 gtgataggcg ctactcgaaa taattccata atccccgagc ggctctctcg aacaatggac
     1861 agcgatgcaa aggttatgga taaatgctgt aaaagcagca caatcactgc tggtgcaaag
     1921 aaacccgtcg tagtgaattg aatgttggat aaaccgctga tatccgacgt aaagggattc
     1981 actaaaactc cggaagagag agaacgaaac gtagctaagt tttcgtgaac cttcgtaaga
     2041 tcgctttcga gttcgtcgac tcgagccgtg tttggatcac caggcggcag ggcctggcgc
     2101 agctcctgtg ttttcaccat tgcagattca aggtcggtct gaagggaacc agtgtcttcc
     2161 tgaccttgtt cagcgacaga ctgcagggcg agtcgattga cttcatcgac atatattctt
     2221 gctgcagact gaatgtaacc gatctggaag gggtccactt cattatggta aataacaaac
     2281 tctgcttgct gattgctctc aatcgtctcc ataggggttt ccgggacgac gatcacgagg
     2341 tcagtttgat tgagggcaag gcgtgatagc gccaacgctt catcagtttc cacaccctga
     2401 tttacgatgg tcgagctgaa ggcctgggtg aatatttgca tttgttcctg aaaggcactt
     2461 ggatcctggg aaacatatgt tgtgcgcaac gcgcggtttt ggtcagggta agccagcccg
     2521 aagaggaaca tgatgaggaa aggacccagg accatggtca ggatcagttt gggctgtcga
     2581 aaaatttcag tcaactcttt ggtcagaaac gaggagatcc gaatcagaaa cttaagcatg
     2641 ggcggactcc ttttcgatga ttcttacaaa tacatcgtca aatgggggcg atatctcttc
     2701 gatcgtttcg atattcaaat tctgcgcctt ggaccaatcc agtagtaagg gcatggctgt
     2761 actggcttca tcaacgataa cttcaatatc ctggtcagta atcactttca ctttactttg
     2821 cacgaagggc aggttttcca attgatggcg ttgttcgggc ctgagttctt ctgtcgtttt
     2881 gattccaacg atttccccgc caaatgcctt tttgcgcaga ttttctggtg tggcaaccat
     2941 caacaatctt ccctcataca ttacgccgac caggtcacaa taggcggctt ctcctacgta
     3001 ttgtgtcgtg acaaacaacg tgtgtccttc ttcctgcagt tctttaaagt aatcccaaaa
     3061 tttccggcgc aggatcgggt cgattcctgc cgttggctca tccagaaata aaagttcagg
     3121 gttatgtacc attgttgccg ccaggctgag gcgtcttttc attccaccag agagatcacg
     3181 ggccaatttg tttctgtgct ctgccagctc cacgaaatcg agaagcttat ttaaacggtt
     3241 tgctcgaaac aggcttaccc cataaaagga tgctgaaaag tttaaatttt cccaaaccgt
     3301 gagttccgga tataagacaa atccctggac gagatacccg atctgttctt tgtctgactg
     3361 tgaaaagttg gtgggttctt taccaagtac agacacgcta ccagaggttg gttcataaaa
     3421 tcccgtcaac aatcggaccg tagtcgtctt cccgcagccg ctcggaccaa taaatccaaa
     3481 aattttgccg cgcggcacgt acaggtcaag agactgcacg gcaacgctct cgccaaatgt
     3541 ttttgtgaga tccttcgttt caacaacgtt ttctgctata tcattatttg tttgattgtt
     3601 catgtttgcc ttgtctgctt ctttgcatag ttttacttac ccaataaata cgccttgatt
     3661 aaggtttcat aagttatata tcactcattc catatcaatc aataagggat ttccctattc
     3721 ggtctcgaca ccggccttga atccaacctg ttgttttccc gctcacttaa taaaaagaca
     3781 ggtcgcctgc ttgattcacg agcagtgacc tgtcgtaatt tcgaagtagt cgactc
//
LOCUS       DASTJP010000027         6761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1578800, whole genome shotgun sequence.
ACCESSION   DASTJP010000027 DASTJP010000000
VERSION     DASTJP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578800"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..1261)
                     /locus_tag="VFQ23_00720"
     CDS             complement(290..1261)
                     /locus_tag="VFQ23_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9905120.1"
                     /translation="MKKIAVTLVALLVVLFALYMTVGYFVYDKLSKVTAGGGENSENI
                     PSNFVNRVDEWAAFDVSPYFMPEFEVIHFSSRQDGLTLTGWFVANPADAPAVILTHGL
                     NGCKCSSRVLTIAGMLHRNGFNVLMYDMRDHGESDVEDGRAAIGNEEYQDLLGAWDWL
                     INEKHFSPESIGVFGESLGAGTTLIAFGQEPRLAAAFVDSPYSDLPQIIDEELARNHY
                     PLFLAPGGIFMARIIAGDNLISFSPRDAILHDAGRPIYIVHGTGDTRIGVHHTRQLAE
                     LAQQTDANVEVWIPDDIGHVEAVFALPDEYEQRLVAFFRESLDGFQE"
     gene            complement(1316..1771)
                     /locus_tag="VFQ23_00725"
     CDS             complement(1316..1771)
                     /locus_tag="VFQ23_00725"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9905121.1"
                     /translation="MTKVVRSHQVLVHAPLQVVFDYVSDLTRHPEWSGGELKIESVAP
                     GPIAVGKEYRSRGEVAIQKDRPNTLQVTQYESPHTFGFAAQDPDFGRVTHVFTFAERS
                     GGVLVTRTMTVNLNPLAAFGFRFLVYPLIGRPSMEKAMSRLKAKLEAQP"
     gene            complement(1877..1999)
                     /locus_tag="VFQ23_00730"
     CDS             complement(1877..1999)
                     /locus_tag="VFQ23_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905122.1"
                     /translation="MPIIIPPPLNQISLASLQSFNKPGQPYELHRQLFGVQGVR"
     gene            2374..5172
                     /gene="alaS"
                     /locus_tag="VFQ23_00735"
     CDS             2374..5172
                     /gene="alaS"
                     /locus_tag="VFQ23_00735"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET9905123.1"
                     /translation="MNKKLTGNEIRQTFIDFFAEHKHTVVPSSSLVPGGDATLLFTNS
                     GMVQFKDVFLGTDKRPYTRAVDSQKCMRVAGKHNDLEDVGRDDTHHTFFEMLGNWSFG
                     DYYKKEAIEWAWQLLTDVWGLKKENTWATVFKDDKGNIPSDEEAADYWRAQPGFVQDH
                     VLYFGRKDNFWEMAEIGPCGPCSEIHYDRGMKYCDKQHIEGHVCQVNGDCKRFLELWN
                     LVFIQYNRLGPDTLEPLPARHVDTGMGFERIVSVLQDVDSNYKTDLFTKALDVLRGLT
                     GASESEMLADFTPYRVICDHTRAAAFLIADGVVPGNVGRNYVARMIIRRAARFGTKIG
                     LNEPFLAQVAEAVIDTYGDFYPELTKSRVAILDNLTREEIRFARTIETGTAHLENLLS
                     GLRASNQTTLDGHRAFDLYATYGLPFEISRDIAWEQGLDVDEAGFKTAMDEHRLASGG
                     GKAMGKSGGEDAEFFSGILKDLQKHGKLGEHGVEYDPYTSPRVEGEILALIVDGTSVQ
                     SASFGEQVQVILPKTGFYIESGGQIDDTGFIRSLPSPAGRGAGGGGNWEIEVTGMRRA
                     SAGVIVHIGEVISGQPKVGDRAIAEVDMTRRHDIMRNHTATHLLHAALHQVLGEHARQ
                     AGSLVAPDRLRFDFNHPEAMTPDQIERVERIVNDAVAADMEVLPKLKSREDAIAEGAM
                     ALFGEKYGETVRTITIRPPSFDMSLEETNVHPSAVPQHEHRYSYELCGGTHLDRTSDV
                     GAFLLTSEGSAAAGVRRIEAITGRGAYDLIARRFKALKQTASTLKSSVDEVPRKVESL
                     QDEVAELKKQLAALRNEAAQSRFSGQVENVQKVGDVNVLAMEVPNANVDTLRSLSDKF
                     REKYPKNGVAVMTSGTTIIAVVTEDLVKQGLKAGDLISAIGGRGGGRPNMAQGSLPND
                     SNTGVLEKVAEAVEEKLK"
     gene            5277..5822
                     /locus_tag="VFQ23_00740"
     CDS             5277..5822
                     /locus_tag="VFQ23_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905124.1"
                     /translation="MSRLNKFISITILVLFILACNFVTEPINDVQNIAGTAQSFASAL
                     PVETLQALASQVASQVPVETFQALPSAVPSLEAFATQFGNLFNPQGTPVEVWREIPIM
                     PQATAGQEFDGETYSFKVNATSTEVQDYYNAELPKLGWEALSMPTQGDATLMVFSKGN
                     NILTITVAPNQSEIVVLLVMA"
     gene            6056..>6761
                     /locus_tag="VFQ23_00745"
     CDS             6056..>6761
                     /locus_tag="VFQ23_00745"
                     /inference="COORDINATES: protein motif:HMM:NF023866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HET9905125.1"
                     /translation="MFTKRHALLSGILFVLLFTSACAGAATPAPSSPSIDYIPYEPAA
                     TEPAATEAPAYSPENEAPFYNPDSTSPNAGSPQPNHEPYDMFFRDYGVNPSIDTEYDN
                     LSTFALDVDTGSYTIMRNYLSEGNLPPPESVRVEEYVNFFDQGYESPPAHQAFGINID
                     GAPSPFTQTERYQMLRVGIQGYQVADYERKDASLTFVIDISGSMDMDNRLGLVKRSLE
                     LLVEQLRPSDRVGIVVY"
BASE COUNT         1720 a         1661 c         1703 g         1677 t
ORIGIN      
        1 agagattaca ggcatataaa atcaaaataa caaaaatgcc taatattact aatcggataa
       61 gttgacccat atttacgtcc ctttcagttt gtgacgtcta ccgtaatatc tcaagaacga
      121 accatgggaa aattgaccac aggactctta ttttatgagt atagcattaa tgtaaattaa
      181 cgcaataatt cagcgttacc atctaatgac tcttaacgag agaataggga aaattctaca
      241 agcaaaaaga tcaaaaaaga cggcgagcta gcgtccgaga gagatctgac tattcctgaa
      301 agccatccaa tgactctcga aagaatgcaa ccagccgttg ttcgtattca tctggtaaag
      361 caaaaacggc ttcaacatga ccgatgtcat ctggaatcca gacttcgaca ttcgcatccg
      421 tttgttgtgc aagctctgcc aattgacggg tgtgatggac cccaattctc gtgtcgcctg
      481 tgccatgcac aatgtagatc ggacgccctg cgtcatgtag aatggcatcc ctcggactga
      541 aggaaattag gttatcaccc gcgatgatcc tcgccatgaa gataccacct ggggcgagaa
      601 aaaggggata atgattccgc gctaattcct cgtcaatgat ttgtggtaga tcagaatagg
      661 gtgagtctac aaaagcggcc gcgagacgcg gttcctgccc aaatgcgatc aatgtcgtcc
      721 ctgcgccaag agattcgccg aaaacaccga tgctctctgg agagaagtgt ttttcgttta
      781 tcagccaatc ccaggctcca agtaaatcct gatattcttc atttccaatt gccgcgcgtc
      841 catcctcaac gtctgattcg ccgtgatcgc gcatatcgta catgaggaca ttaaatccat
      901 ttcgatgcag catcccagcg attgtaagta cacgcgaact gcacttgcag ccattcaatc
      961 catgcgtcag tatgacggcg ggcgcatccg ctggatttgc gacgaaccaa cctgtgagtg
     1021 tgagtccgtc ctgacggctg ctgaagtgaa tcacttcaaa ttcaggcatg aaatacggtg
     1081 agacgtcgaa cgctgcccac tcatcgaccc tgtttacaaa gttagaggga atattttcag
     1141 agttttcacc tcccccggct gttacttttg ataatttgtc gtatacaaaa tagccaaccg
     1201 tcatatagag tgcaaaaagt actaccagga gggctaccag cgttacagca atttttttca
     1261 tctttattta gaaccccatc acgcgaaatg agatagcaac cttgccggcg gagtttcagg
     1321 gttgcgcttc aagttttgct ttgagcctcg acatcgcctt ttccatcgat ggtcttccaa
     1381 tcagaggata gaccaggaaa cgaaacccga acgctgccag aggattcaaa ttcacggtca
     1441 tagtccgggt tacaagaacg ccgccacttc gttccgcgaa tgtaaagaca tgggtgaccc
     1501 gtccaaagtc tgggtcctgt gctgcgaatc caaacgtatg aggagattca tattgtgtga
     1561 cttgcaacgt gttagggcga tctttctgaa tagcaacttc tccacgagat cgatattcct
     1621 ttcccaccgc aattggacca ggcgccacag attcaatttt aagctcacct ccgctccact
     1681 cgggatgacg cgtcaggtca gaaacataat caaacaccac ttgcagtggg gcatggacaa
     1741 gaacttgatg tgatcgcact acttttgtca ttgtggctcc cttttgaatg tgacaattcg
     1801 ttgctacata gagaaggcaa cgtggtgacg attctcccta tccggttttc tttgcggaaa
     1861 tcgaagggga gcgggtttac ctcactccct ggactccgaa cagttgcctg tgcagttcgt
     1921 agggttggcc tggcttgttg aatgattgaa gcgatgccag gctgatctga ttcaacggcg
     1981 gagggatgat gatgggcatg gcagcttatt ttaacgaatt cctgcggtta tgcatgcctc
     2041 aattttcagt gtaggtcact ctactgaatt gctttagccg gcaaaggctg aaccagtccg
     2101 gaatcaggat atttccgctt atttttgcct ggaagaatga tataatcttt tttatggaac
     2161 aaacagtaaa atctttattt acttcattta ttgccgctgc tgcctcagct ttaagcgtag
     2221 cccaaatcgg ggctgctcaa tgaacctgtg gtagcgtcag actgaatttg aaattcaagt
     2281 cccgaactca atcaggttcg ggactttttg ttttccgtca atttcgaaat ttgtccattg
     2341 gtataattcc aaaattcaat cactgaggtc cgaatgaata agaaactaac tggcaatgag
     2401 atccgccaaa cttttataga tttttttgct gaacacaaac ataccgttgt gccgtcttct
     2461 tcgcttgtcc caggcgggga tgcgacgctg ctgtttacca actcggggat ggtgcaattc
     2521 aaggatgtgt tcctcggcac tgataagcga ccgtataccc gtgctgtcga ctcgcagaag
     2581 tgtatgcgtg tggcaggcaa acacaacgac cttgaagatg taggtcgtga cgatacccac
     2641 cacacgtttt ttgagatgct cggaaactgg tccttcggtg attattacaa aaaagaagcc
     2701 attgaatggg cctggcagtt gctcaccgat gtttggggac tgaagaaaga gaacacatgg
     2761 gcgaccgtct tcaaggacga caagggcaat atcccctcgg atgaagaggc ggcggactat
     2821 tggcgggcac agccggggtt cgtgcaagat cacgtgctct atttcggacg caaggacaat
     2881 ttctgggaga tggcagaaat cggtccctgt ggtccgtgtt ccgaaatcca ctatgaccgt
     2941 ggcatgaagt attgcgacaa gcagcatatt gaaggtcacg tttgccaggt caatggagat
     3001 tgcaaacgat tcctcgaact ctggaatctg gtcttcatcc aatacaaccg cctcggtccc
     3061 gatacgcttg agccacttcc cgcgcggcac gtggacacgg gcatgggctt tgaacggatt
     3121 gtctccgttc tgcaggatgt cgactcaaat tacaagaccg atctcttcac caaagcgttg
     3181 gacgtgttgc gcgggcttac tggggcaagc gaaagtgaaa tgcttgcgga tttcacgccg
     3241 tatcgcgtca tttgtgacca cacccgcgcg gctgcgtttc tcatcgcgga tggagtcgtg
     3301 ccgggtaacg tcggtcgaaa ttatgttgcg cgcatgatca ttcggcgcgc agctcggttc
     3361 gggaccaaga tcggtctcaa tgaaccattt ctcgcccagg tcgcggaagc ggtgattgat
     3421 acctatggtg acttttatcc agaactgacg aagagccgcg tcgcgatcct tgataatttg
     3481 actcgcgaag aaattcgatt cgcgcggacg attgaaacgg gtaccgcaca tttggagaat
     3541 ctactctcag gcctgcgtgc ctcaaatcaa actaccctcg atggtcatcg cgcattcgac
     3601 ttgtatgcca cgtacggttt gccatttgaa atttcccgcg atatcgcatg ggagcagggg
     3661 ctggatgtgg acgaggcagg attcaaaacc gcgatggatg aacatcgtct tgcgtccggt
     3721 ggcggcaaag ccatgggcaa gtcgggtggt gaggatgctg aatttttctc cggcattttg
     3781 aaagacttgc aaaagcacgg caaactcggt gaacatggtg ttgaatatga tccctatacc
     3841 agcccgcgcg tcgaaggtga aattctagct ctcatcgtag atggcacctc ggttcaatca
     3901 gcatcctttg gtgaacaagt acaggtcatc ctgcccaaga cgggcttcta tatcgaatct
     3961 ggtggacaaa tagatgatac gggatttatt cgttctctcc cctcgcccgc tgggagaggg
     4021 gcagggggtg ggggcaattg ggaaatcgaa gtcaccggga tgcgtcgcgc ttctgcgggc
     4081 gtgatcgtcc acatcggtga agtgatctcc ggtcagccaa aggtcgggga tcgggcgatc
     4141 gccgaagtgg acatgactcg tcgtcatgac atcatgcgca accacaccgc gacgcatctt
     4201 cttcatgcgg cgttgcatca agtgctgggt gaacatgcgc gtcaggcggg ctcgctggtc
     4261 gcgccggatc gtctgcgctt cgacttcaat catcctgaag cgatgacgcc ggatcaaatc
     4321 gagcgtgtcg aacgaatcgt caatgacgcg gtcgcggctg atatggaagt gctgccaaag
     4381 ctcaaatccc gcgaggacgc gatcgctgaa ggtgcgatgg cactcttcgg cgagaaatac
     4441 ggtgaaacag ttcgcaccat cacaatcagg ccacccagct ttgatatgtc tcttgaggaa
     4501 acgaatgtac atcccagcgc ggtcccacaa cacgaacaca gatattctta tgaactctgt
     4561 ggcggtacac acctcgaccg cacatcggat gtgggcgcgt tcctccttac aagtgaaggc
     4621 tctgcagctg ctggcgtacg ccgtatcgag gcgatcaccg gccgcggggc atatgacctg
     4681 atcgctcgaa gattcaaagc gctaaagcag actgcctcaa ccttgaaatc ttcggtcgat
     4741 gaagtgccgc gcaaagtgga atctctgcag gatgaagttg ccgaattgaa gaaacaatta
     4801 gctgccttgc ggaacgaagc tgcgcaatcg aggtttagcg gccaagtgga aaacgttcaa
     4861 aaggtcggcg atgtaaatgt actggcaatg gaagtgccca atgccaatgt agatacgttg
     4921 agatctctct ccgataaatt tcgcgagaag tacccgaaga acggtgtcgc ggtgatgacc
     4981 tccggtacaa ccatcattgc cgttgtgaca gaagatttgg tcaagcaagg actcaaagca
     5041 ggcgacctga tctccgccat cggtggccgt ggtgggggac gcccaaatat ggcacaaggc
     5101 agcctgccga acgactccaa cactggtgtc ttggaaaagg ttgccgaagc tgtcgaagag
     5161 aagttgaaat aaccaataga gcggacggtt gtccgctcta tttctgatag gtagataaat
     5221 ccattttccc caactttcgc aatggattat gattgcacga gaaaaggagc tagaagatgt
     5281 cccgattaaa caaattcata tcgatcacga tcctcgtttt gttcatcctc gcctgtaact
     5341 ttgttacaga gcccattaac gatgtccaga atattgccgg aacagcgcag tctttcgcct
     5401 ccgccttgcc ggttgaaacg cttcaggcgt tggcaagtca ggtggcaagc caggttcccg
     5461 ttgaaacgtt ccaggcgctt ccgtccgcgg tgccatccct ggaggcattt gcaacgcaat
     5521 ttggaaattt attcaatcca cagggaacgc cggtagaggt atggcgagag attccgatca
     5581 tgccgcaagc aaccgcgggt caggaatttg acggcgaaac ctacagtttc aaagttaatg
     5641 ccacctctac agaagtacaa gactattaca acgctgagct accgaaactt ggctgggaag
     5701 cgttaagtat gccgactcaa ggagatgcca cactgatggt attctcaaag ggaaacaata
     5761 ttctcaccat taccgtcgct ccgaaccaaa gtgagatcgt tgtcttgctc gtaatggcat
     5821 aagattaagc gacaaacaat ctcgaaggat ggctgcacaa gccatccttt ttgtttatct
     5881 ggggaacttt tattttaggg cttcgtccat tcatcaggta cgctcaataa ttttgatttc
     5941 ttcattttga aaggagggct tatgaattaa tatgttcttc ggcgcgcaac aggaacgccg
     6001 acctgttgtc agcccacgcg cgttgcatct aataaactaa tttaggagaa tagatatgtt
     6061 caccaaacgt catgccctgc tctcgggtat cctcttcgtg ttgttgttca cttctgcctg
     6121 tgctggtgct gccactcccg ctccttcaag tccatccatt gattacatcc cctatgaacc
     6181 cgccgcgacg gaacctgcag ctacggaggc gcccgcgtat agtccggaaa atgaagcgcc
     6241 gttctacaat cccgattcca cgtcaccaaa tgctggtagt cctcagccga atcatgaacc
     6301 ctatgatatg ttctttagag actatggagt caacccatcc atcgatacag agtacgataa
     6361 cctttccacc ttcgcgttgg atgtagatac gggttcctat acgatcatgc gtaactacct
     6421 gagtgaaggc aatctgccgc cgcctgaatc agtgcgggtt gaagaatatg tcaacttctt
     6481 cgaccaggga tatgaaagcc ctcctgcgca tcaggcattc ggaatcaaca ttgatggagc
     6541 gccgtcgccc ttcacacaaa ccgaacgcta tcaaatgttg agagtgggaa ttcaaggata
     6601 tcaggtcgcc gattatgagc gcaaggacgc ttctcttacg tttgtgatcg atatttctgg
     6661 ctccatggat atggataatc gtttgggact ggtcaagcgt tcgcttgaac tgcttgttga
     6721 gcagttgcgg ccgagtgata gagtcggcat cgtagtttat g
//
LOCUS       DASTJP010000028         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1260622, whole genome shotgun sequence.
ACCESSION   DASTJP010000028 DASTJP010000000
VERSION     DASTJP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260622"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFQ23_00750"
     CDS             complement(<1..517)
                     /locus_tag="VFQ23_00750"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET9905126.1"
                     /translation="MKTLVHSTRPVKVIVFVLLALALTVSAANAWGNYRQQEFLKTIK
                     GKAPTRKGEVNQEVINASTVRPVTNAAAPTVGFTPQIRLGFDVGDQWEPAIAADRFGH
                     VYMLYPQYEGVPGCANCSNPTQILQISNDRGQTWGSPFVLYPAGATTGGQWDPQVVVD
                     PVDGRTVYTTFM"
     gene            735..2207
                     /locus_tag="VFQ23_00755"
     CDS             735..2207
                     /locus_tag="VFQ23_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET9905127.1"
                     /translation="MSQTINILFVAAEAEPFVKVGGLGDVAGTLPRALRALSSEVTGD
                     VKLDIRLVLPLHSVIRQDSLRPVGIYSIPRGESDVQVEAFEGVLDGMPVYFLSGEPIR
                     ASGTVYSSNNKLDAEKYVFFSLAALELPRQINWTPSIIHVNDWHTALASYGNLAKRWA
                     DKKHRTVSIVEIHNLPFLGPDVKEILESYGLPLANTDLPDWARVMPMPLGLWASDTII
                     TVSPTYAEEILHAEFGCGLQDFLTNRTSTLYGILNGLDTVSFNPQTDTALAANYSVDT
                     LSARPKNKAALQERVGLPVQSEAPVFGIVSRMDQAKGIDIALKGLKLMAKQNWQLVLL
                     GAGDATLEASARKLQEEMPDRVRVETRYDAKLARQIYGGADILLMPSRYEPCGISQMI
                     AMRYGCVPLVRAVGGLHDTVTDSETGFVFVDARVKSFNDAVRRALSLYPNRSRWSELQ
                     QAGMAIDFSWTASAKKYFELYKKLLESTSASLGASNGVPR"
     gene            2275..>3140
                     /locus_tag="VFQ23_00760"
     CDS             2275..>3140
                     /locus_tag="VFQ23_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9905128.1"
                     /translation="MKTRAVILAGGEGSRLGALTEKRTKPAVPFAGKYRIIDFPLSNC
                     VNSGIFDVLILAQYRPQSLIQHIGAGAPWDLNRDFTGGVKILTPYKARHDSDWYAGTA
                     DAVQQNFSFIKEGKPDLVLILSGDHIYTMDYDPMIAFHMDHRADLTMGTISVPLAEAS
                     RFGILATNAKYKVTAFVEKPENPPSTLANMGVYLFNRERLDRVLWEDRQMDGSSHDFG
                     KDILPRLIKSKARVFAFPYSGYWMDVGTMYSYWQAHMDMLSPSPPLKLHSRSWVIHTR
                     SEERPPARLPATA"
BASE COUNT          761 a          775 c          808 g          796 t
ORIGIN      
        1 gcatgaacgt tgtgtaaacg gttcggccat ctaccgggtc taccacgact tgaggatccc
       61 attgtccgcc tgttgtcgcg ccggcaggat agagtacgaa aggcgagccc caggtttgcc
      121 cgcgatcatt actgatctgc aggatctgag tggggtttga acagttcgca cagccgggga
      181 caccttcata ttgcggatac aacatataaa cgtgaccgaa ccgatctgca gcaatggctg
      241 gttcccactg atcacccaca tcaaaaccaa gccggatctg tggcgtgaaa ccaactgtgg
      301 gcgctgccgc gtttgtcacc gggcgaaccg tggaagcatt gatgacttcc tgattcactt
      361 ctcccttgcg cgttggcgcc ttgcctttga tggttttcag aaactcctgt tgtcgataat
      421 tcccccaggc atttgcggcg ctgaccgtta gcgcaagtgc cagcaagacg aacacaatta
      481 cttttaccgg acgtgttgag tgaactaacg ttttcatgca attctccttt gggtaaattt
      541 gccgcggatt acttaaatgc tgaagggcaa aagatcttat ttcaggagcg ggacgtaatg
      601 cggcagattg taaaaatatt gtataacaag tttcccaaaa aaactattag cgaatgcaac
      661 aaccgagttt ttggttagac tgtctcacga aaactgactt aagggtctct tgaatttcgt
      721 tgtacaattg gtatatgtcc cagaccatca atatcttgtt cgtcgctgcc gaagcggaac
      781 cgtttgtcaa ggtgggtggg ttaggggatg tagccggcac tttaccgcgg gcgctgcgtg
      841 cgctctcatc agaagtcacg ggggatgtta aactcgacat acgccttgtc ttgcctttgc
      901 attccgtcat ccgacaggat tctcttcgac cggtcggaat ctattcgatc ccgcgcggag
      961 aatcagacgt gcaggtggag gcgtttgaag gtgtgctgga tgggatgcca gtctattttc
     1021 tcagcgggga acccattcgc gccagtggaa cagtctattc ctcgaacaac aagttggatg
     1081 ctgaaaaata tgtcttcttc tcgctggctg ccctggaact gccgcgccaa atcaattgga
     1141 ctcccagcat cattcatgta aacgactggc atacggcgtt ggcatcctat ggcaatctgg
     1201 cgaaacgatg ggcggataaa aagcatcgaa cggtgtccat tgtagaaatc cacaatttgc
     1261 cttttttagg acctgatgtc aaagaaatct tggaatcgta tggcctgcct ctggccaata
     1321 ccgatcttcc ggattgggcg cgcgtcatgc ccatgcccct tgggctatgg gcttcagaca
     1381 caatcattac cgtttcccca acttatgcag aggaaatcct gcacgcggaa tttgggtgcg
     1441 gcttgcagga ttttctcacg aatcgcacct caaccttgta cggcattttg aacggacttg
     1501 atactgtttc atttaatcct caaacggaca ctgcactggc tgcgaactac agcgtggata
     1561 cactttccgc gcgtccgaaa aataaggctg cattacagga acgtgtcgga ctgccagttc
     1621 aatccgaagc tcctgtgttt ggaatcgtct cgcgaatgga ccaggccaaa ggtatcgaca
     1681 tcgcgcttaa aggactcaag ctgatggcga aacagaattg gcaactggta ttgttggggg
     1741 ctggagatgc cacactcgaa gccagcgcaa gaaaattaca agaggagatg ccagaccggg
     1801 tgcgtgtaga gacacggtat gatgctaaac ttgcacggca aatttatggc ggcgcagata
     1861 tattgctgat gccttcccga tatgagccat gcggaatttc gcaaatgatc gccatgcgct
     1921 atggttgcgt acctctggtc cgtgcggtgg gtggcctgca tgatacggtc acagatagcg
     1981 agacaggctt tgtcttcgtg gatgccagag tgaagtcatt caatgatgcg gtgagacgtg
     2041 ccctgagtct gtacccgaat cggtcgcgtt ggtctgaact gcaacaggca gggatggcga
     2101 ttgatttctc ctggacggca tctgcgaaaa agtatttcga attatataaa aaactgctgg
     2161 agtcaacttc ggcttcgtta ggtgcatcga acggcgtacc ccggtaatca gattgatttt
     2221 catgaacgtt tggcaaagat cagcaatcaa aatccatatc gaagaggata attcatgaag
     2281 actcgtgctg taattcttgc gggtggtgaa ggctccaggc tcggtgcgct tacagaaaaa
     2341 cgtaccaagc ccgccgtgcc gtttgcagga aaatatcgca tcatcgattt cccactttcg
     2401 aactgtgtca actcgggtat ttttgatgtt ttgatcctgg cccaatatcg cccacaatcg
     2461 ctcattcaac atattggcgc aggcgcgcca tgggatctga accgtgactt caccggtgga
     2521 gtaaagatac tgactccgta taaggcccgc catgattctg attggtatgc aggaacggca
     2581 gatgctgttc agcaaaactt tagcttcatc aaagagggca agcccgatct cgtgctgatt
     2641 ctctccggag atcacattta caccatggac tacgatccaa tgatcgcctt ccacatggac
     2701 catcgtgccg accttactat gggtacgatc agtgttcctt tggcagaagc ttcgcgtttt
     2761 gggatccttg ctaccaatgc caaatataag gttacggcgt tcgtggagaa gcctgagaat
     2821 ccaccttcga cgcttgcaaa tatgggtgtc tacctcttca atcgcgagcg gcttgacaga
     2881 gttttatggg aagatcgtca gatggatggt tcctcgcatg attttggaaa agacatcctt
     2941 ccccgtctca taaaatccaa agcgcgtgta tttgcgtttc cctatagtgg gtattggatg
     3001 gatgtgggca ccatgtattc ctattggcag gcacatatgg atatgctctc accatcaccg
     3061 ccacttaaac tccacagccg tagctgggtc atccatacac gaagtgaaga gcgcccgcca
     3121 gcgcgccttc cagcgaccgc
//
LOCUS       DASTJP010000029        21311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_943252, whole genome shotgun sequence.
ACCESSION   DASTJP010000029 DASTJP010000000
VERSION     DASTJP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 21311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21311
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943252"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..977)
                     /locus_tag="VFQ23_00765"
     CDS             complement(45..977)
                     /locus_tag="VFQ23_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HET9905129.1"
                     /translation="MAHSLPVSNLDRRNAMQESVISNVVLPLSIAVIMVTLGMTLTFG
                     DFRRIITQPKPVLIGLFCQMVLLPVLGFAVAGIFALPAVYAISLILLAVSPDGATSNL
                     IIHAGDGDRALGITLTAITNMLAFLTIPFGLGIAYSIYGTGALDINFPVLDTMIQVAV
                     ITLIPTLLGMGIRIWKPEFAENSKRWSKIFATAFLFLVILALVIQNWDVIVRDGPRFA
                     PAFIVLNVASLIVGYFVPKLAGINYVQAMTIAIETGLQNSTVSITVAISLLNNNDMAV
                     IPGLYAIWMYVTGFTLAFWMARRNTGEPMKQAIA"
     gene            complement(1135..2466)
                     /gene="ahcY"
                     /locus_tag="VFQ23_00770"
     CDS             complement(1135..2466)
                     /gene="ahcY"
                     /locus_tag="VFQ23_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9905130.1"
                     /translation="MSNYDIKDLNQAEGGRRRIDWAEREMPVLRSIRERFKVEKPLKG
                     LRVSACLHVTTETANLMHTLQEGGADIVLTASNPLSTQDDVAAALVNQFEIPTYAIKG
                     EDNVTYYKHIRAALEHRPHMTMDDGADLVSSLFFIEMGRFEKLEPSLSSWAKGLSDDE
                     RKELLAGVIGGTEETTTGVIRLKAMEADKVLKFPVIAVNDAQTKHLFDNRYGTGQSTI
                     DGIIRATNVLLAGKNFVVAGYGWCGRGLASRARGMGAQVIVTEVDPMKALEAVMDGYQ
                     VMPMADAARIGDFLCTVTGDINVIDEEHFLSMKDGAIVANSGHFNVEINIPALEKLST
                     GKPSLVRPFVDQYTTKDGRKINILGEGRLINLASAEGHPASVMDMSFANQALSAEYMA
                     KNADQLEKKVYSVPTEIDKEIARLKLEAMGVRIDALTAEQTHYLNSWEEGT"
     gene            complement(2558..3511)
                     /locus_tag="VFQ23_00775"
     CDS             complement(2558..3511)
                     /locus_tag="VFQ23_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET9905131.1"
                     /translation="MDILLTGSVAYDYLMTFPGHFKEQILPERLGSISLSFLVESMSK
                     QRGGIAPNIAYTMALLGEKARVMATVGEDFSDYREWLESKGVDTSLMMVVPGEFTASF
                     FATTDHSSAQIASFYPGAMAHSSMQSLKNLEKKPDLVMVSPSAPDAMMKFRAECHELG
                     IPYLYDPSQQVLRLEGHELARDMEGANFLFCNDYEFGLISKKTGWSLDQMLEHVKVLV
                     ITRGKDGADLYAGEDAVHIPTVPEDEIVDPTGVGDAFRGGFLTGYSRGFDWKLCGEIG
                     SLAAVYCLEQKGPQKHSYTKAEFIERFRKHFDDAGELDSLL"
     gene            complement(3696..4454)
                     /locus_tag="VFQ23_00780"
     CDS             complement(3696..4454)
                     /locus_tag="VFQ23_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9905132.1"
                     /translation="MLMFQSPTPTRYDLRFTIAGIPVRVHPLFWLITLLLGSSGDLIL
                     IPIWLFVIFVSILIHELGHALAFRRYGQRSHIVLHFAGGLTIPEPAYWGSGVANVYLS
                     PNQNIFISLAGPVAGFLLAGLTIATVLLTGGSVGISWLLGFLPLPLAAQLSFGGEILN
                     LLVRLMLWVNIFWGLVNLLPVFPLDGGQVARNILIQYDPINGVRKSLWLSVIVGGIFA
                     LVGLVFFQSVFMALLFGLLAFQSYQSLQGRALGY"
     gene            complement(4474..5541)
                     /gene="mtnA"
                     /locus_tag="VFQ23_00785"
     CDS             complement(4474..5541)
                     /gene="mtnA"
                     /locus_tag="VFQ23_00785"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET9905133.1"
                     /translation="MRTVFWEDNQLKMIDQRILPARFEIVSYADHKSVAQAIKDMVVR
                     GAPAIGAAAAFGLALAGYESKSSSTSDLMADLLSASTTLKAARPTAVNLAWAVDRVMG
                     KALDKSNQVTDSESDMIRQLVLDEAQRIADEDVEINKRMAEYGAALIEDGDTIIHHCN
                     TGALATVDWGTALGVIRTAHEQGKKIHVLVDETRPRLQGARLTAWELDQYGIPYEIIS
                     DNMSGYFLKAGKAQKVFFGADRVAANGDVANKIGTYMLSLAAHDNGVPAYAVVPTSTV
                     DLFLEHGDLIPIEERDPQEVLGIQFQGERIAPVEAEARNIAFDVTPNRLITGIVTEHG
                     VVYPPFGDNLKKYVLKNIIER"
     gene            5650..7380
                     /locus_tag="VFQ23_00790"
     CDS             5650..7380
                     /locus_tag="VFQ23_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9905134.1"
                     /translation="MFTQKRTLLFLTIMVMCSLLIAACQPAAQPSGTEEPSTSPTSEK
                     SITIVIAEDPPSFNPMVADTGYDALVMELVMLGMTDVDPQGNVFPELAAELPTLENGG
                     VVIDDDAGTMSVTWKMREDINWQDATPVTANDVIFTWNAISDPDNGIWIPGSDYIDSV
                     EQVDQYSFVVNYNTIYPGYLTQFGGEQLAVWPAHYCDATQGFVAWDCGREPLSNGPYV
                     LDEWVEGDHMTFTRNENFYAEVKPEIERVIVRIVPDDSVRKTMMLQGDADIDMWINVN
                     TASELEGSEAAKVSLSPTDRWVMRLFMNQAEKGTTDAAATPHPILSDVRVRQAIRSAV
                     DVETINNEIFHGLGNLVWTEFFRPPYQCEVTRPSFDPEASKALLEEAGWTDTDGDGIR
                     ECHGCANAEEGYPMEMEFITYAEFGEPLELTQQLIAEMLGNIGMKLNITVVEGSVLWD
                     LAENGGIEQSGNFDINIWDDGYAGVDPTDYVWEIYSQEAIEPGGGWNIARWDNPAVDA
                     LIDEAYTLDEGTRQETFCAIGDFINEEVPIIHLFTVPNMDAYAARLEGVQSSVNDLVT
                     WNIADWKLKE"
     gene            7511..8548
                     /locus_tag="VFQ23_00795"
     CDS             7511..8548
                     /locus_tag="VFQ23_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905135.1"
                     /translation="MTTYIARRLIQTVGLLFLLSILLFTLVNLAPGGPLSAYGGRRIR
                     PERAEILKRQFGLDQPLPIQYLYWLAGNDWLQVDTDGDGVIDERGSRRGILRGDFGFS
                     YRTREPVLQEIGVRFPNTIYLMSITMIVALAVAIPIGIYSAVKQYSIFDFFATTFSFA
                     GQAIPEFWLGLLLILIFYAWLQNPWTGEPLFPSGGISSLDGGFSLPDRLSHLVLPVLT
                     GALGWIAWYSRFLRSSLLEILPLNYLKSARARGLPERTVIYKHALRNALVPIVTLLAL
                     DLPYVFTGAVLIETIFSWPGMGRLYYQAAVERDYPLLMAVLIIGAAFIILCNLLADIL
                     YAYLDPRVRYA"
     gene            8705..9628
                     /locus_tag="VFQ23_00800"
     CDS             8705..9628
                     /locus_tag="VFQ23_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905136.1"
                     /translation="MTTYASIETTPQTVDLAQNVWKRFKRHRGAIAGTVIFGMLVMAC
                     VFAFLSPYDPEASELSVEFLPPSPSHWFGTDALGRDLFTRILYGGRISLTVGGVAVAI
                     SLLIGVPIGALAGYYGGRLDSVLMRLTDAFLSLPSFLVLILLAAILREVELPLFQRNS
                     VLTISLVIGVLSWMTFARLVRAAFLTLRELDYVSAARALGSTDRRIIFGHILPNGIGV
                     VIVEATLQLGYAIIQESGLSFLGFGIQQPTPSWGNLISAAQDHFIKYPWLAIFPGLMI
                     FITIISVNYIGDGLRDAFDPYKVLETVGEYT"
     gene            9649..9900
                     /locus_tag="VFQ23_00805"
     CDS             9649..9900
                     /locus_tag="VFQ23_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905137.1"
                     /translation="MNNNLLINIVGWTGSILYLLAYTLVSLKKTEGDSLLYQGINIFA
                     GTLLVIYTLYLGAYATTGLNAVWVVIGLLTLGRKWLTRN"
     gene            9882..10652
                     /locus_tag="VFQ23_00810"
     CDS             9882..10652
                     /locus_tag="VFQ23_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905138.1"
                     /translation="MAYTKLIPRHSPGAKVEEISGAEKYKLLIPAGPANKYRIAQLDD
                     YAQKLRGQFPLRSPLTLRVSARASSQSIPGTWGFGLWNDPFGLSLGLGGNPFRLPALP
                     NAVWFFGASEESYLSFRGVDMASPVRPPANGFIAQTFRSPKFHPSLILAGLALPFSRK
                     VTRRILGKIIDEDGMRLSVDVTQWHKYKLEWRENRVSFEVDNLQVFESRVSPNPPLGL
                     VIWIDNQYAAFTPEGKIGFGVLKNPEPAWLEIDELTVS"
     gene            complement(10649..11680)
                     /locus_tag="VFQ23_00815"
     CDS             complement(10649..11680)
                     /locus_tag="VFQ23_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET9905139.1"
                     /translation="MNINFGMWRKASWQLIKMNDKKEWDALDVISKWLIATRSAVTLV
                     TVYSCIIAGILAARDGYFSWIPFLIITLGLFIAHGTNNLLNDYTDYSRGVDKDNYFRT
                     QYGVHPLVQGFFTKPQQIRWFMVSGVLATLAGIYALFYTNFDRTVIALFVIGAVVLLA
                     YTYPFKYWGLGELAIFLIWGPIMIAGVYYVLSGVWNWNVVLAGIPYGLGVASINVAKH
                     IDKHDDDKAKGVGTFPVRVGEALARRVDQIAIILIYVVIAYLIFVPRYFTPAMLLVFL
                     AFKDAITVVKVLNNPKPAQPPAGWPAWPVWFSGFAFQHNRQFGGYILLGLIIDALLRT
                     YLPNFWPIL"
     gene            11820..12464
                     /locus_tag="VFQ23_00820"
     CDS             11820..12464
                     /locus_tag="VFQ23_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9905140.1"
                     /translation="MSEGKNEYIPALSFRVLTPFYDFIQKYIVRDVRYKSLLIQQAKL
                     KAGQRVLDLGCGTGTLAIMAKQAQHKADVFGLDADPEMLKMAYNKRDEAKLNVTFDVG
                     FTNHLPYPDESFDRILSSIMIHHLKTPEKEETAREVYRVLKPGGQLHIIDFGKPYTWY
                     GKLLGPFLHKFEEANDNIEGRLPEIFGAPGLKTKVVGHFWTFFGDLAFLKGEKL"
     gene            12486..13028
                     /locus_tag="VFQ23_00825"
     CDS             12486..13028
                     /locus_tag="VFQ23_00825"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9905141.1"
                     /translation="MTRIESSIIMPISIDDVFSFLSERKSHLNFIPRMIALDQTSPGD
                     FGRVGATADGMLNYFGLRIPVQYEIIEHQPNKQLAMRGKMGPVNFRDGYMLSRHAEGT
                     EIKFWLDLNPTGWAKIFSPFAGLIGKLHAWETLRNLKRELAIWRSSTDEETLAPDAHR
                     PGGSVPGSAGVASPPRSSSQ"
     gene            13037..13588
                     /locus_tag="VFQ23_00830"
     CDS             13037..13588
                     /locus_tag="VFQ23_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9905142.1"
                     /translation="MAIDLYKPFTNPITKETFRCISSTPEVYTMEWTVHPGGYVPFEH
                     VHVCQDEVFHVEKGEIRARVNGKTLFGKPGDSLTAPRGIKHIAYNDRDEKLVCIVDYK
                     PGLDHYTVMQCFAGLTLEGKIARNGLVSVPHIMYLLKRANAESLPRPAFAPDIAFRLG
                     MNVFYLVGSMLGWEEFYKRYTRK"
     gene            13629..14657
                     /locus_tag="VFQ23_00835"
     CDS             13629..14657
                     /locus_tag="VFQ23_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET9905143.1"
                     /translation="MDVKLWWFALQKPVKEYDKKVWDSFDVIAKWLISTRATVTQLTL
                     MAGVIAGLLALRDGHFNFPLWLAMTLGIYFAHSSENLVNDYIDYTRGIDEDNYYRGQY
                     GIHPLVHKFWTKQEWARWFFTAGTIAVLCGIFVLIYTGFSPIVIGLFLFGAAILPTYA
                     WPLKYWGLGELLIYANWGMVLIPGIYSILAGEIPSNLPSIIMAGMAFGFGFGSFNFGK
                     HIDKIEADTKKGVGSLPVRLGERAARMFNIFTVILSYALVLYLVFVTHFYSPTVLLVF
                     LAAGRGWRVVQLLSQPRPKEAPPGFALWPRWFSTPQLLHIRLFGGLYILGIILDILLR
                     MNVPGFWQ"
     gene            14670..15575
                     /locus_tag="VFQ23_00840"
     CDS             14670..15575
                     /locus_tag="VFQ23_00840"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9905144.1"
                     /translation="MKIAITGGTGFIGRHLANELVEQGHDVIVIARGLYSRNTQPVAG
                     ATFVSMDANHTDKLQEVFNGCDAVAHCAGTSVEDERQTFEQLHVNGTRSAVTAAEKAN
                     VKKFVLVSYLNVRPNTSSAYYSTKWQGEEVVRASKLNYTILKAGLIYGPGDHLLHNLS
                     NLFQKMPVFATVGITEKTVRLIAVEDLVKIISAVLVDENRLSRQTVAVVGPEEFPFST
                     AARRISRAMGKPFLLILPFPVLFQQILAWFSERFMPKPLITKAQVQMLADGISKPLPD
                     SEMIPESMAPKTLFTEEQIRKGLPK"
     gene            complement(15628..16923)
                     /locus_tag="VFQ23_00845"
     CDS             complement(15628..16923)
                     /locus_tag="VFQ23_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9905145.1"
                     /translation="MLPIKYETLSLSNLLNPEIRANPYPFYARLRAEDPVHWDDELGF
                     WVFTRYSDIASMYTDNRFSRAQGLMSNFERLGEAEKLIAEPVYRSFAKTMFYADPPYH
                     THLRGLMNNAFTPRRVERMRPYIQNIVDELLDHVEEQGQMDVVRDLAYPLPVMVIAEL
                     LGLPTSDRARFKGWSDDLFAILGTVRRSPHLMDRAAQSLGEMTAYMTELSRKRREDPR
                     DDLLSVLLSVVAEDEELDCPHPHHAQGELPPDHRTPSQLTQDELVANINILLSTGHET
                     TTHLIGNGVLALLQHPEQLQNLRNNPRLVFSAIEEIMRYDNPVQITYRSAIQDAEISG
                     KQIHKGDLINSILGAANRDLERYTEPDRFDITRNEGRHLNFGLGIHFCIGASLVRLEA
                     EIAFNTILRRFPKLQLATDQLEWQEHPIFRGLKALPLAF"
     gene            complement(17101..19908)
                     /locus_tag="VFQ23_00850"
     CDS             complement(17101..19908)
                     /locus_tag="VFQ23_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9905146.1"
                     /translation="MLSDILHWLRPPVFPEDEDKTRVAMALNAILMIGVGGALFYSVF
                     ILSVARSEIKSIAFSLFPILFMAVLWALMRSGKVYLASVLFVSLSWLNITIAAIADGY
                     GIRGTSLYGQVLIVIVAGLIINWRASVVFALLNVASGLIMIYASNEGLLPPRGVIQTE
                     FTIWSANAVFSISAAFVLGITLKSLHNAIQRAFISESHYRMLFEAAPDGILIVDQNNQ
                     IIMANSAIYQMTGYSPDDVIRHSPLDFVAPDELTIRPPRPLSELKTAGSNKRERTLIH
                     KDGSRLNVIISSSYMPDGQFQYIIQDITERKSIEAALRTSEEKFAKSFQSSPDAVTIS
                     FVDTGKFIEVNEGFCRMSGYTREEALGRSAEELNIWEDVQQRNEMVAILQTNGRVRDF
                     ETSLRRKNGEALNCLLTVESVEISGQNCMVVITRDVTERKRMEEELRLSEERYRLVSS
                     VISDYIYSTVRDGDQWHQNWIAGAFERITGYTLTEFNNRGGWLSILHPDDLGIDADAM
                     RKLLNNEDTVAEIRIIRKDQSICWVRSYAHPVWDERQARLVGIYGAVQDITAQKQIEQ
                     ELLLSEERYRLVSSVISDYTFSNIQNERGEIVLNWVAGAFEQISGYTLEEFNARGGWI
                     CTVYPDDLEQDAQDMEALRRNQKVISEIRTIHKNGKIRWMKSYAHPVWDEERQELIGI
                     YGAVQDITDRKQIEQEREKLIKDLEAKNQELEQFTYTVSHDLKAPLITIKGFLGFLGV
                     DARAGDEKRVEADIQRINEATDKMHELLTDLLELSRIGRLMNPPEMILFQSLVAEAIA
                     LTEGRLQERGIGLIVKEDLPSVYGDHQRLLEVLQNLIDNAAKFMGDQPTPLIEIGRHS
                     EKYDGFETIYIRDNGIGLAPEFHERIFGLFNRLNPKIDGTGIGLTLVKRIVEFHGGRI
                     WLQSQVGEGTTFYFTLPVGPP"
     gene            complement(20102..21109)
                     /locus_tag="VFQ23_00855"
     CDS             complement(20102..21109)
                     /locus_tag="VFQ23_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9905147.1"
                     /translation="MKILIIGGTGLISSACSDLAVARGHELVIVNRSASAHYAVPEGA
                     IVLNADVYTEEANLASLVAGHRFDAVVDFLAFTVADVERDLRLFQGKTDQFVFISSAS
                     AYQKPPKHYLITEETPLENPYWLYSRNKIACEERLMKEYREQGFPVTIIRPSLTYGTS
                     QIPLVGASWSHPYTVVDRIKRGRKVIVPGDGTSLWVLTWNADFATGLMGLLGNPNAIG
                     EAFHITSDEVLTWNQIFLEVYQALGLEPNLVHVSSEMIIQHDPEKLGTLLGDKVNSVV
                     FDNSKIKRFVPDFNCRVNWAEGLRRSLDSFQAHPEFQTIDDNMNGLWDRIIASYEKID
                     A"
     gene            complement(21122..>21311)
                     /locus_tag="VFQ23_00860"
     CDS             complement(21122..>21311)
                     /locus_tag="VFQ23_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HET9905148.1"
                     /translation="GVLQYLAPTIQFLIGIFIYKEAFDQSRLIGFSIVWLALIIFWVE
                     NYLANRMIATPLPELGKR"
BASE COUNT         5520 a         5374 c         5041 g         5376 t
ORIGIN      
        1 ttattatgct tctactcgac ctgcatattt aagacaatat tatcttacgc gattgcctgc
       61 ttcataggtt cgccagtatt tcggcgtgcc atccagaaag caagagtgaa gccggtcaca
      121 tacatccaga tggcatacaa accggggatg actgccatgt cgttgttgtt caataaagaa
      181 atagcaaccg tgatggaaac agtggaattt tgcaggccag tctcgatagc gatcgtcatg
      241 gcctgtacat aattaatccc tgccaacttg ggaacaaaat atcccacaat cagtgaggcg
      301 acattcaaaa ctataaacgc tggggcaaac cgcggaccat ctcttacaat cacgtcccaa
      361 ttttgaataa caagcgccag aatcacaaga aaaagaaagg cggtcgcgaa gattttcgac
      421 cagcgcttgc tgttctcagc aaattccggc ttccatattc gaattcccat acccagcaag
      481 gtcgggatta gagtaatcac tgccacctgt atcatcgtat ccagtacggg gaaattaata
      541 tcgagcgcgc cagttccgta gatcgaataa gcaatgccca gcccaaaggg gatcgttaag
      601 aaggccagca tgttcgtgat ggcagtcaat gttatgccaa gtgcgcggtc gccatcgccg
      661 gcgtggatga tcaagtttga tgtcgcgccg tctggcgaga ccgccaataa aatgagacta
      721 attgcataca cagccggaag cgcaaaaatg cccgctacgg caaatcccag tactggaagc
      781 aggaccattt ggcaaaacag accgataagc acaggttttg gttgtgtaat gatccgccga
      841 aaatcgccga aggtcagcgt catgcccaag gtcaccataa tgactgcaat tgacaatggg
      901 agaactacat ttgaaattac actctcttgc atggcgtttc tcctatctaa attagataca
      961 gggagtgaat gtgcaatccc atgactctat gaaaccccat aatgcatgtt aagtatcggc
     1021 aaaattctag agcaaggttt tgaataaatt ttggagaata aaaatcacaa taaaaagcct
     1081 cgtggacaat gctgtccacg aggctcaatc gtaaatcaca acctgtgact tgacctatgt
     1141 tccttcttcc catgagttca gatagtgagt ctgctcggcg gtaagtgcat cgattcttac
     1201 tcccatggct tccagcttga gacgggcaat ttccttatcg atttcggttg gaacggagta
     1261 aactttcttt tccaactgat ctgcattctt tgccatatat tctgctgaca acgcctggtt
     1321 ggcaaaggac atatccatca cggaagcagg gtgtccctca gcagatgcga ggttgatcaa
     1381 tcgtccttca ccaaggatat tgattttgcg tccatctttc gtggtgtatt gatcgacaaa
     1441 cggacggacc agacttggct tgcctgtgct caacttctca agcgccggga tattgatctc
     1501 aacgttgaag tgtccggaat tagcaacaat tgcaccatct ttcatcgata agaaatgttc
     1561 ctcatcaatg acattgatat cgccagttac agtacacagg aaatcaccga tccgggcggc
     1621 atcagccata ggcattacct gataaccgtc catcactgct tcgagggcct tcatcggatc
     1681 gacttccgtg acgatgacct gcgcacccat accgcgtgcc cgtgaggcaa ggccacgtcc
     1741 gcaccagcca tagcctgcca ccacaaagtt cttgccagcc agcagcacat tcgtcgcgcg
     1801 gatgattccg tcaatggtgg attgacctgt accataccgg ttatcgaata aatgcttggt
     1861 ctgcgcgtca ttaaccgcga tcacggggaa cttcaaaact ttgtccgctt ccatcgcctt
     1921 gagccgaata actcccgtgg ttgtttcctc tgtgccgcca atgacgccag caagtaactc
     1981 tttacgttca tcatcggaga gtcctttcgc ccaactcgaa agcgatggct caagcttttc
     2041 gaagcgaccc atctcaataa agaagaggga ggaaacaaga tcggctccat catccattgt
     2101 catatggggc cggtgttcca gcgcggctcg aatatgcttg taataggtaa cgttatcttc
     2161 gcctttgatc gcgtaggtgg gaatctcaaa ttgattcacc agcgcagccg caacatcatc
     2221 ctgggtggag agtgggttcg aagcggtgag gacaatatcc gcgccgcctt cctgcaatgt
     2281 atgcatcaag ttcgcggttt ccgttgttac atgcagacaa gctgaaactc gcaagccctt
     2341 cagcggcttc tccactttga atcgctcacg gatcgagcgc aggaccggca tctcgcgttc
     2401 cgcccaatcg atgcggcgac gcccgccttc cgcctgatta aggtctttga tatcgtaatt
     2461 gctcattgtt gctccttctt tctggatcgc ggattttagt ccgcaacttt atagttagtt
     2521 tagttaagcg gactgcagtc cgcgcacctg aaaattttta caacaaacta tccaactccc
     2581 cagcgtcatc aaaatgcttt cgaaaacgtt cgatgaattc tgcttttgta taactgtgct
     2641 tctgtggacc cttctgctcc aaacaataga ccgcggccag cgaaccaatt tcaccgcaaa
     2701 gcttccagtc gaagccacgg gaatagcccg ttaaaaatcc gccgcgaaat gcatcaccca
     2761 ctccggtggg gtcaacgatt tcatcttcag gcacagttgg gatgtgaacc gcgtcttccc
     2821 cggcgtaaag atctgcacca tcttttccac gagtgatgac cagcactttt acgtgctcca
     2881 acatttggtc caggctccaa ccggtcttct ttgagatcag tccaaactcg tagtcgttgc
     2941 aaaataaaaa gtttgcgcct tccatatcgc gcgctaactc atgaccttcc agccgaagga
     3001 cttgctggct gggatcatac agatacggaa ttccgagttc atggcattcc gcgcgaaact
     3061 tcatcatagc atccggcgcg ctcggtgaca ccatcaccaa gtcaggcttc ttctccaaat
     3121 tcttcaagga ttgcattgaa gaatgcgcca ttgctcccgg gtaaaacgaa gcgatttgtg
     3181 cggaagagtg atctgtcgtc gcaaagaagg atgcggtaaa ttcgcctgga accaccatca
     3241 tcaatgacgt atccacgcct ttcgattcga gccattcccg gtaatctgaa aaatcctcgc
     3301 cgaccgttgc catcacgcgt gctttttcgc caagcagagc cattgtatac gcgatattcg
     3361 gagcgattcc gcctcgctgt ttggacatgg attcgacaag aaaggaaagg ctgatcgacc
     3421 ctaagcgttc gggaagaatt tgttccttga agtgacccgg aaaggtcatc agataatcat
     3481 aagcaacaga gccggtaaga agaatatcca tatgtttcct cccgtcatgc gacgtgcatt
     3541 tgtagcatgt aagaaaactc ctcgcaaaga cggtgtatat tcgcagtaaa aggcgtggcg
     3601 cttgagcgcc acgccattgt cagcgggcaa gagtttatca cgccataacg gtcttgtcta
     3661 gaaaataata caagtaattt ttctaaaacc tgatatcaat atccaagggc acggccctgc
     3721 aacgattgat aactctggaa ggctaataag ccaaacagga gcgccataaa cacgctttgg
     3781 aagaatacta aacctaccag ggcaaaaatc ccacccacaa tgactgaaag ccagagagac
     3841 ttgcgtactc cattgatagg gtcatattgg atgagaatat tccgcgccac ctgtccacca
     3901 tcgagcggaa agaccggcag aaggttgact aatccccaga aaatgttcac ccaaagcatc
     3961 agcctgacca gtagattcaa tatctcacca ccaaaggaaa gttgcgctgc gagtggcaag
     4021 gggagaaacc ctaacaacca tgatatacca accgacccac ctgtgaggag aacagtggcg
     4081 atggttaaac ctgcaagcag aaatccggca acaggaccgg ccaatgaaat aaatatattt
     4141 tgattgggag acaggtagac atttgcaacc ccacttcccc agtaggcagg ttctggtatg
     4201 gtcaagccac ctgcaaagtg aagcacaata tgcgatctct gcccgtaacg gcggaacgcg
     4261 agcgcgtgtc ctaactcatg aatcaggata gacacaaaaa taacaaacaa ccagatcggt
     4321 atcaggataa gatcaccgga agagcccaat agaagcgtga tcagccaaaa caatggatgc
     4381 acgcggacag ggattccagc aatggtaaaa cgaagatcat aacgagttgg ggtaggcgat
     4441 tggaacatca gcatgaggtt aaattatctt tctttatctt tcgataatat tcttcaaaac
     4501 atacttcttt agattatccc caaaaggcgg atagaccact ccgtgttcgg tgacaatccc
     4561 ggtgatgagt cgatttggcg tgacatcaaa cgcgatgttg cgtgcttcgg cttcgacagg
     4621 tgcgattcgt tctccctgaa attgaattcc cagcacctct tgagggtcgc gttcttcaat
     4681 ggggatcaag tcaccatgtt cgaggaaaag gtcaaccgtc gaggtcggca ctaccgcata
     4741 tgcagggacg ccattatcat gcgcggcaag cgataacatg taggtcccga tcttattggc
     4801 cacatctcca ttcgcagcga cgcggtcggc accaaaaaaa accttctggg ctttaccagc
     4861 tttcaaaaaa tagccggaca tattatcgct gatgatctca taagggatgc catattgatc
     4921 cagctcccag gccgtcaggc gtgcgccttg caagcgtggg cgggtttcat ccaccagcac
     4981 atggatcttc tttccctgct catgtgcggt gcgaatgact ccaagcgcag ttccccaatc
     5041 tacggtggcg agggcgcctg tgttgcagtg gtgaatgatc gtatcaccat cctcaataag
     5101 cgccgcgcca tattcagcca tgcgcttgtt gatttccacg tcttcgtccg caatacgctg
     5161 agcctcgtct aaaactaact ggcgaatcat gtccgactcg ctgtcagtca cttggttcga
     5221 tttatcgagt gctttgccca ttacacgatc tacggcccac gcaagattca ctgccgtcgg
     5281 gcgcgcagcc ttcagcgtgg tagaagcgga tagtaaatcg gccatcaggt cactcgtcga
     5341 agaagatttg gattcatatc ccgcaagcgc aagcccaaaa gcggccgccg ctccaatcgc
     5401 cggcgcgccg cgcacaacca tatccttgat tgcctgtgca accgatttat gatctgcata
     5461 cgaaacgatc tcaaaacgcg caggcaggat gcgctggtcg atcatcttca gttgattatc
     5521 ttcccaaaat actgttctca tgaactggat tttactcgga aaactctcat ataaaaacct
     5581 tatcatcaaa ggcaaatcgt gcaataatac agggcgtttg caaaatctaa aaaatccgca
     5641 ggaggatcaa tgttcacaca aaagcgtacg ttgctctttc tgaccataat ggtcatgtgt
     5701 agtcttctaa tagcggcatg tcagcccgcc gcccagccat cggggacaga agaaccatcc
     5761 acttcaccca cttctgaaaa aagtatcacg atcgtaatcg ctgaggatcc accatcgttt
     5821 aacccgatgg tagcagatac aggctatgac gcgctcgtca tggagcttgt catgctcggc
     5881 atgacagacg ttgacccgca gggtaacgtc ttccctgaac tcgcagcaga attgccgaca
     5941 cttgaaaatg gaggggtggt tattgatgac gatgccggaa caatgtctgt cacgtggaag
     6001 atgcgagaag acatcaactg gcaggatgct acaccggtca ccgccaatga cgtgatattc
     6061 acctggaacg ccatttctga cccggacaat ggcatctgga ttcctggcag cgattacatc
     6121 gactcagttg aacaggtaga tcagtattcg tttgttgtca attacaacac gatctatccg
     6181 ggttatctca cacagtttgg cggcgaacag cttgccgtct ggcccgctca ttattgcgac
     6241 gctacgcaag gatttgtcgc ttgggattgt ggacgcgaac ctctcagcaa cggcccgtat
     6301 gtgcttgatg aatgggtgga aggcgatcac atgaccttca cccgaaatga gaatttttat
     6361 gcagaggtca aacccgagat tgaaagagtg atcgtccgga tcgtgccaga tgactctgtc
     6421 cgcaaaacaa tgatgcttca gggcgatgcg gatatcgaca tgtggatcaa cgtcaatacc
     6481 gctagcgagt tggaaggttc ggaggccgca aaagtgagtc tgagtcctac cgatcgctgg
     6541 gtgatgcgtc tgtttatgaa tcaggctgaa aagggaacca ctgacgccgc agctacacct
     6601 cacccgatcc tgtcggatgt gcgcgtacgt caggcgattc gttccgcagt agacgtagag
     6661 acgatcaaca acgagatctt tcatggcttg ggcaacctgg tctggaccga attcttccgg
     6721 ccgccatatc aatgtgaagt gacacgtcct tcctttgacc cggaggcctc aaaagcccta
     6781 ctggaagaag ctggctggac ggatacggat ggtgatggca ttcgagaatg ccatggttgt
     6841 gccaatgccg aagaaggata ccccatggaa atggaattca tcacttatgc tgagtttggc
     6901 gagccgctgg aactcacaca acaattgatc gctgagatgc tcggcaatat cggcatgaaa
     6961 ttgaatatca cggtggtaga aggcagtgtg ctctgggatc ttgctgaaaa cggcggcatc
     7021 gagcaaagtg gcaacttcga tatcaacatc tgggatgatg gttatgccgg cgtggaccct
     7081 accgattatg tatgggaaat ctattcacag gaagccattg aacccggtgg cggatggaac
     7141 atcgcgcgct gggataaccc tgctgtggat gcgttgatcg atgaagcgta cacactagat
     7201 gaaggaaccc gtcaggaaac attctgcgcg attggcgatt tcatcaacga ggaagttcct
     7261 atcattcatc tctttacagt tccaaatatg gacgcctatg ccgctcgcct ggaaggtgtt
     7321 cagtcttcag tcaatgacct ggtcacctgg aatattgccg attggaagtt gaaggaataa
     7381 cttttagccc aggtgaccca aacctcaaca agacttaatc gcgaagcacc ctgagctcac
     7441 gaagaagatt atctatatct tcgcgttctt tgttgtcttc gcggttccat ttactccact
     7501 cttagactag atgactacct acatcgcgcg ccgccttatc caaactgttg gcttgctttt
     7561 tctgctcagt attcttctct tcacgctcgt caacctcgca cccggcggac cgctctcagc
     7621 atatggaggc agacgaatcc gcccggaacg agctgagatt ctcaaacggc agtttggtct
     7681 ggaccagccc cttccaattc aatatctata ctggctcgcc ggcaacgact ggctccaggt
     7741 tgatacagac ggggacggag tcatagacga acgtggctcg cgccgaggca ttctgcgtgg
     7801 cgactttggt ttttcttatc gaacgcgcga acctgttctc caggaaatcg gtgtgcgctt
     7861 tcccaatacg atctatttga tgtccatcac tatgatagtg gcattggcgg tcgcaatccc
     7921 aatcggcatc tattcagctg tcaaacaata ttcgatcttc gatttcttcg caaccacctt
     7981 ctcctttgca ggtcaggcga ttccagaatt ttggcttggg ttgttactca ttctgatttt
     8041 ttacgcgtgg ttgcaaaatc cgtggacggg agaaccgctt tttccgtccg gtggcatatc
     8101 atccctggat ggcggatttt ctctccccga ccggctctcc catctggttc tcccagttct
     8161 gactggagct ctgggctgga tcgcgtggta ttcgcgcttt cttcgttcca gccttcttga
     8221 aatcctcccg ctaaattatc ttaagtctgc acgagcaaga gggttgcccg aacgaactgt
     8281 gatttacaaa cacgcattga ggaatgcctt ggttcccatc gtcacgctgc ttgctctcga
     8341 cctgccatat gttttcaccg gcgcagttct catcgagacg attttttcct ggcctggcat
     8401 gggaaggctt tattatcaag ccgccgtcga gcgtgactac cctctcctta tggcagttct
     8461 catcatcggt gcggccttca tcattctctg caacctgctg gcggatattc tctatgcata
     8521 ccttgacccg agggtgaggt atgcataaca aatgtcaccc ctgcactggc gcacggtaca
     8581 agtgttgtga ggagggcttc accccgacga ttgcgaagca tcctgtaagg aaccaatctc
     8641 gtggtttcag gttagttgag attgccacgg cggctgcgcc gcctcgcaat gactgtcaga
     8701 aactatgacc acctacgcgt ccatagaaac cactccacaa accgtagatc ttgctcaaaa
     8761 cgtttggaaa cggttcaaac gccatcgcgg agcgatcgcg ggtacagtca tatttggtat
     8821 gttagtgatg gcatgtgtat ttgcctttct ttcaccctat gatccggagg catccgagct
     8881 atcggtcgaa tttctacctc catctccttc acactggttc ggcaccgacg cgctcgggcg
     8941 agatctcttt actcgtattc tctatggtgg gcgcatttcg cttactgtgg gcggggtggc
     9001 agttgccatc tccctgctca ttggggttcc tattggtgcg ttggctggct attatggtgg
     9061 aagacttgat tccgttctca tgcgcctcac agatgcattt ttgtcactcc cttcttttct
     9121 tgtcttgata ctactcgcag cgattttgcg agaagttgaa ctccctctat ttcaacgcaa
     9181 tagtgtattg acgatcagcc tcgtcattgg ggttctctca tggatgacgt tcgcacggct
     9241 ggtgcgcgcc gcattcctca ccttacgtga attggactat gtctctgctg cacgcgcgct
     9301 cggctcaaca gacagacgca tcatatttgg tcacattctc cccaacggga tcggcgtggt
     9361 aattgtcgaa gccacattac aattagggta tgccatcata caagagtcag gtttgagctt
     9421 tctaggcttc ggcatacaac aaccaacccc ctcctggggc aatctcatct ctgccgcaca
     9481 ggatcacttc atcaaatatc cgtggctggc gatcttcccg ggtttgatga tcttcatcac
     9541 cattatttcc gtcaattaca tcggcgatgg cctgcgcgat gccttcgatc cgtataaggt
     9601 gctggagacg gttggtgaat atacttaagg aaaattcatt tctatcccat gaacaataac
     9661 cttctaataa acattgtcgg ctggaccggt tcgatactat atctgcttgc atacacttta
     9721 gtctccctga aaaaaactga aggagactcg cttttatatc aaggcatcaa tatcttcgcg
     9781 gggactttgc ttgtaatcta tactctttat ttaggagcat acgcaaccac gggtctcaac
     9841 gctgtatggg ttgtcatcgg tttgctcaca ctcggccgta aatggcttac acgaaactaa
     9901 tccctcgtca ctcgcctggt gcaaaggtag aagaaatttc aggcgcggaa aaatataaac
     9961 tattaatccc ggcggggccc gcaaacaaat atcggatcgc gcaacttgat gactacgcgc
    10021 aaaaactaag agggcagttt cctttgcgct cacctctgac cctcagagta tcggcgcgtg
    10081 cttcgagcca gtctattcca ggaacttggg gatttgggtt atggaatgat ccctttgggc
    10141 tgtctcttgg gcttggaggc aatccgtttc gcttaccagc cctaccgaac gctgtatggt
    10201 tcttcggcgc ttcggaggaa agttatcttt catttcgtgg tgtcgacatg gcttcgcctg
    10261 ttcgaccacc tgcgaatgga tttatcgcgc agacgtttcg ctcaccaaag tttcatccat
    10321 ctttgattct tgccggtctt gctctcccct tttcgcgcaa agtgacgcgg cgaattttgg
    10381 gaaaaatcat cgatgaggat ggaatgcgtc tcagcgtaga tgtcacacaa tggcacaaat
    10441 acaagctgga gtggcgcgag aatcgagttt catttgaagt agataacctc caggtttttg
    10501 agtcgcgcgt gagtccaaac cctccgctgg gactcgtcat atggatcgac aaccaatatg
    10561 cagcattcac ccctgaagga aaaatcgggt tcggagtctt gaaaaacccc gaacccgcgt
    10621 ggttggaaat tgacgaacta acggtcagct aaagaatggg ccagaaattc ggcaagtatg
    10681 tcctcaacag cgcgtcaatg atcagcccca aaagaatgta gccaccaaat tgacggttgt
    10741 gttggaaagc gaaccctgaa aaccaaaccg gccaggcggg ccagcctgca ggaggctgag
    10801 ccggctttgg attgttcaac accttcacaa cagtgatcgc atctttgaag gcaaggaaaa
    10861 caagcagcat cgccggggta aagtatcgtg gcacaaaaat cagataagca attacaacat
    10921 agatcaggat gatcgcgatc tggtcaacgc ggcgggccaa tgcctctccc actcgcaccg
    10981 ggaaagtacc aacacctttg gctttatcat catcgtgttt gtcaatatgc ttcgcaacgt
    11041 tgatgctcgc cacgcctaat ccatagggaa tgccggctaa gacaacgttc cagttccaca
    11101 cccccgagag aacatagtag acacccgcga tcataatggg accccaaatg aggaagatgg
    11161 caagttcccc taatccccag tatttaaagg gataagtgta cgcaagcaac accactgcac
    11221 caattacaaa taaagcaatt acggttcgat caaaattggt ataaaacaga gcatatatac
    11281 cagccagtgt agccagcacc ccactgacca tgaaccatct gatctgctga ggcttggtga
    11341 agaatccctg taccaatgga tgcactccgt attgggtgcg gaagtagttg tctttgtcca
    11401 caccacgcga ataatcggtg taatcattca gaagattatt cgtcccgtgg gcgataaata
    11461 atccgagtgt aatgatcagg aatgggatcc acgaaaaata gccgtcacgc gctgccagga
    11521 tcccggcgat aatacaggaa tatacggtca ccagcgtcac agcagaacgc gtggcaatca
    11581 accattttga aataacatcg agagcatccc actctttctt gtcattcatt ttgatcaatt
    11641 gccatgaggc tttccgccac attccaaaat tgatattcat acttctctcc tattgataga
    11701 atgattgagg atttgataag ggctctgaaa aattatacta cttatttgtg aaatagggta
    11761 caattggtta taaatggaga gtttggtaca atctcactcg atgcaggaga agcaaggtta
    11821 tgtctgaagg taaaaacgaa tacattcccg ctttaagctt cagggttttg accccttttt
    11881 atgattttat tcaaaaatac atcgtacgtg atgttcgcta taaaagcctt ctgattcaac
    11941 aggcaaagct caaagccggt caacgcgtcc tagacctggg ttgtgggacc ggaacgctgg
    12001 caatcatggc caagcaggcg cagcataagg cggacgtctt tggtttggac gcggaccctg
    12061 aaatgctcaa aatggcgtat aacaagaggg atgaagcgaa acttaatgta acctttgatg
    12121 ttgggtttac aaaccacctg ccctacccgg atgaatcctt cgaccgtatt ctctcaagca
    12181 ttatgatcca tcatctaaaa acaccagaga aagaggagac tgcacgcgag gtttatcggg
    12241 tcctcaagcc ggggggtcaa ctccacatca tcgactttgg aaagccttac acctggtatg
    12301 gaaagttgct cggtcccttc ctgcataagt ttgaagaggc gaatgacaac atcgaaggac
    12361 gcctgcccga gatctttggt gcacccgggt tgaagacaaa agtcgtcggt cacttctgga
    12421 cgttctttgg ggatctggca tttctaaaag gggagaagct ctaagtgcaa aggatttaag
    12481 agcaaatgac gcgcattgag tccagcatta ttatgccaat atctattgat gatgtattct
    12541 catttttgag tgaacgcaaa agccatttga atttcatccc gcgaatgatc gcacttgatc
    12601 aaacttctcc aggtgatttc ggacgagtgg gtgcaacggc tgatggaatg ctcaattact
    12661 tcggtctccg aatccctgtg cagtatgaaa tcatcgagca tcagccaaat aaacagcttg
    12721 ccatgagagg caagatgggt ccggtgaact tcagagatgg ttatatgttg agccgacacg
    12781 ctgaaggaac agaaatcaag ttctggcttg atctcaatcc tacgggatgg gcgaaaattt
    12841 tttcgccgtt tgcggggctg atcggcaaac tccacgcctg ggaaacgctt agaaacttaa
    12901 agagagaatt ggcaatttgg cgttcttcta ctgatgagga gacacttgca cctgatgcac
    12961 accgccccgg tggttcggtg ccggggagtg caggtgttgc ttcgccgccg cgcagctcat
    13021 ctcaataata tgaatcatgg caattgacct ttacaagcct tttacgaatc caattaccaa
    13081 agaaacgttt cgctgtattt catcgactcc agaagtctac acgatggaat ggacagtaca
    13141 tcccggaggg tatgtcccgt tcgagcacgt tcatgtctgt caggatgaag tctttcacgt
    13201 ggaaaaaggc gaaattcgtg ccagagtaaa cggaaaaact ttattcggca aaccgggcga
    13261 cagtttgacc gcgccacgtg gaattaagca tattgcctac aacgaccgcg acgaaaaact
    13321 ggtctgcatt gttgactata agcctggact tgatcactac acggttatgc aatgttttgc
    13381 agggttaacg ctcgaaggaa agatcgcccg aaatggtctg gtgagtgttc cgcacatcat
    13441 gtatctctta aagagagcaa atgctgagtc gcttccacga cctgcctttg cacccgacat
    13501 tgcgtttcgt ctcggtatga atgttttcta tctggtcggc agtatgttgg gctgggaaga
    13561 gttctataaa cgatatacac gaaaatagtg catctgaagc agcagtatga acaggagaaa
    13621 cgataagcat ggacgttaaa ctttggtggt ttgcacttca gaaaccggtc aaggaatacg
    13681 acaaaaaagt ctgggattcg tttgatgtca tagccaagtg gctgatttcg acacgcgcaa
    13741 ccgtcacaca gttaacactc atggctggcg tgattgcagg tctactggca ctgcgcgatg
    13801 gacactttaa cttcccgctg tggctggcaa tgacgttggg catctacttt gctcactcat
    13861 ccgaaaacct ggtcaacgat tacatcgatt acacccgcgg catcgacgag gacaattact
    13921 atcgcggaca atatggaatc catccccttg tgcacaagtt ttggaccaag caggaatggg
    13981 cgcgctggtt tttcacagca gggacgatcg cggtactgtg cggcatcttt gtgctgattt
    14041 atacgggttt ttcacccatt gtgattggct tgttcctatt tggagctgcg atcctcccta
    14101 cttatgcctg gcccctcaag tattggggac ttggtgaact cctcatttat gcaaattggg
    14161 ggatggtgtt gattcccggc atctattcga ttctggctgg cgaaatccct tcgaatcttc
    14221 ccagcatcat tatggcaggc atggcgttcg ggtttggatt cggaagcttt aactttggca
    14281 aacatattga taagatcgaa gccgacacga aaaagggcgt gggttcgctc cccgttcggt
    14341 tgggtgaacg cgcggcgcgg atgttcaaca tctttacagt gatcctgtcg tatgcactgg
    14401 tgctctatct ggtctttgta acgcatttct attcaccaac agtactcctc gtttttctcg
    14461 ctgcaggcag agggtggaga gtggttcaat tgctgagtca gcctcgtcca aaagaagcgc
    14521 cgcccggctt cgcattatgg ccgcgctggt tctcaacgcc acagttattg catatccgct
    14581 tgtttggcgg cttatacatc ctgggcatta ttctcgacat tctgctccga atgaacgtgc
    14641 ccggcttctg gcaatagaaa gacaacagca tgaaaatcgc aattactggt ggcactgggt
    14701 ttattgggag gcatttggcg aatgagcttg tagaacaagg tcatgacgtg attgttatcg
    14761 cccgcggatt gtactcacga aacacacagc ccgtcgctgg agcaacgttt gtgagtatgg
    14821 atgccaacca tacagacaaa ctgcaggaag tcttcaatgg ctgcgacgcg gtcgcacact
    14881 gcgccggaac cagtgtggaa gacgaacgtc agacttttga acaactgcat gtaaatggaa
    14941 cgcggtccgc cgtgacagct gcagagaaag caaatgtaaa gaagtttgta ttggtcagct
    15001 atctcaacgt ccggcccaat acttcatctg catattactc cacaaaatgg cagggtgaag
    15061 aagtggtgcg tgcatccaaa ttgaactaca cgattctcaa agccggcctc atttatggac
    15121 caggagacca tctacttcac aatctcagca acctgtttca aaaaatgccg gtctttgcta
    15181 ccgttgggat tacagagaag actgttcgat tgatcgcggt tgaagacctg gtaaaaatta
    15241 tcagcgcggt gctcgtggat gaaaatcgcc tctcgcgcca gacggtggca gttgtcggtc
    15301 ccgaagagtt tcctttcagc acggctgcac gtcgaatttc cagagcgatg ggaaaaccgt
    15361 tcctattgat tttgcctttc cccgttttgt ttcagcaaat tctggcatgg ttctctgaac
    15421 gatttatgcc aaagccgttg atcaccaaag cccaagtcca aatgctggca gatggcatca
    15481 gcaagccatt gccagatagc gaaatgatcc cggaaagtat ggcacccaaa accttattta
    15541 ctgaggaaca gattcgcaaa gggctgccga agtaaactta tctaacctgt agagactgga
    15601 gcttttgttg atctccggtc tctttttcta aaaagctaac ggcaaggcct tgagaccgcg
    15661 aaatatgggg tgttcctgcc actctaattg atctgttgcg agctgcagct tggggaaacg
    15721 acgaagaatc gtattaaatg caatttcagc ctcgagccgc accagtgaag ccccgataca
    15781 gaaatggata cccaatccaa agttgagatg ccgcccttca ttgcgggtga tatcgaatcg
    15841 atctggttcg gtgtatcgct ccaggtcacg gttggcagcg cccaggatgg aattgatcag
    15901 atctccctta tggatttgtt tgccgctgat ttcggcatcc tggattgctg agcgatatgt
    15961 aatctgaacc gggttatcgt agcgcatgat ttcttcaatg gcagagaaaa ccaagcgcgg
    16021 gttattccgc agattttgca gttgttcggg atgttgcagc aacgctaata ccccattgcc
    16081 gatcaggtgc gtggtcgtct cgtgaccggt actcagtaga atgttgatat tcgctaccag
    16141 ttcgtcctgc gtaagctgag aaggggttcg atggtcaggc ggcagttctc cctgcgcgtg
    16201 atgtggatgt ggacagtcca attcttcatc ttcagctact acacttaata aaacactcag
    16261 cagatcatct cggggatctt ctcggcgctt gcgactcagt tcagtcatat aggctgtcat
    16321 ttcacccaaa ctctgagcag ccctgtccat caggtgaggc gaacgacgga cagtccccag
    16381 aatcgcaaag agatcgtccg accacccttt gaaacgcgca cgatcacttg tgggtaagcc
    16441 caataattca gcaataacca tcaccggcaa tggataagcg aggtcacgca caacatccat
    16501 ttgcccctgc tcttccacat ggtccagcaa ctcatctacg atattttgaa tataagggcg
    16561 catccgttcg acgcggcgcg gcgtaaaggc attgttcatt aatccacgca gatgtgtatg
    16621 gtaaggtgga tctgcataaa acatggtttt agcaaaggaa cgatacaccg gttcagcaat
    16681 aagcttttca gcttcgccca gccgctcaaa attactcatc aacccctgcg ctcgtgagaa
    16741 acgattatcg gtgtacatcg aagcaatatc ggaataacgc gtgaaaaccc aaaatcccag
    16801 ctcatcatcc cagtgcaccg gatcttcagc ccgcagccga gcatagaatg gataaggatt
    16861 ggcgcgaatc tcaggattca acaaatttga cagactcagc gtctcatact ttatcggtag
    16921 cattcagact ccttggatca gtacagcgcc gcccaaagtt tgcgcgatta tacctaaaag
    16981 gacttcagtt gttgtactga ttcggtgaaa cttcatacat gttcaaccag atagtatact
    17041 accaatctgt gaaattcggc acaatccaag cgtgatttct gtggcgaaag aataattcga
    17101 tcagggtggg ccaaccggca gggtgaagta gaaggtggtt ccctctccca cttggctctg
    17161 cagccagatc ctgcctccgt gaaattcaac aattcgtttt acaagagtca gtccaatgcc
    17221 ggttccatcg attttggggt ttaggcgatt gaaaagtccg aatatgcgct catgaaattc
    17281 gggcgccaag ccgatcccat tgtcacggat ataaatcgtc tcaaaaccgt catatttttc
    17341 actgtgccga cctatctcga ttagcggagt cggctgatct cccataaatt ttgccgcatt
    17401 atcgatgagg ttttgaagaa cctctaatag gcgctggtga tctccataaa cactcgggag
    17461 atcttctttc acgatcaacc ctattccacg ctcctgcaaa cgaccttcgg tcaatgcgat
    17521 ggcctcagcg accagcgact gaaacaggat catttccggc gggttcatca acctgccgat
    17581 tcgtgatagc tcaagcaggt cagtcaataa ctcgtgcatt ttatcagtgg cttcgttgat
    17641 ccgttgaata tcggcttcaa cgcgtttctc atctccagca cgagcatcta caccgagaaa
    17701 ccccagaaag ccctttatcg taataagggg agctttcagg tcatgagaca cggtataggt
    17761 aaattgctca agctcctggt tcttcgcttc caaatccttt atcaatttct cacgttcctg
    17821 ctcgatttgt ttacgatccg taatatcctg aaccgccccg tagattccga tcagctcctg
    17881 cctttcttca tcccaaactg ggtgtgcata gcttttcatc caacggattt tgccattctt
    17941 atgaatcgtg cggatttcag atatgacctt ctgattacgt cttaaggctt ccatatcctg
    18001 cgcatcctgt tcaaggtcat cggggtaaac ggtacaaatc caaccaccac gcgcgttgaa
    18061 ttcttctaat gtatatcctg atatctgttc aaacgcgccc gccacccaat tgagcacgat
    18121 ttcacctcgc tcattttgga tattggaaaa ggtgtaatcc gatattacgg aagagaccag
    18181 gcgatatcgc tcttcactca gaagtaattc ctgctcaatc tgtttttgtg cggtaatatc
    18241 ctgcaccgcg ccgtaaatcc ctaccagacg agcctgtcgc tcgtcccaaa ccggatgagc
    18301 atagcttcgg acccaacaaa tggattgatc cttgcggatg atccgaattt cagctacagt
    18361 atcctcgtta ttcagaagtt ttctcatggc atcggcgtcg atccccagat catcgggatg
    18421 gagtatggac aaccaccctc ctctattgtt gaattccgtc agggtgtagc cggtgattct
    18481 ttcaaacgct cctgcaatcc aattttggtg ccattgatct ccatctcgta cagtcgaata
    18541 gatatagtca gagattacag atgaaacgag acggtagcgt tcttcactca gccgcaattc
    18601 ctcttccatt cttttccgct cggtcacatc acgcgtaatt acaaccatac aattctgccc
    18661 actaatttcc accgactcta cagtcagcaa gcaattcaga gcttcgccgt ttttccgtcg
    18721 aagggatgtt tcaaaatcac ggactctgcc atttgtttga agaatagcaa ccatttcatt
    18781 tcgctgttgc acatcttccc aaatattcag ttcctcggct gagcgtccca gtgcttcttc
    18841 gcgggtatag ccactcattc ggcaaaagcc ttcattcacc tcgataaatt ttccggtatc
    18901 aacaaaagaa atcgtaactg catcggggct ggactggaag gatttggcga acttttcctc
    18961 cgaggtccgc agggccgcct ctatgctctt gcgttcggta atatcctgaa taatatattg
    19021 aaattgtcca tcgggcatgt aactgctgct aataattacg tttagacgcg acccatcttt
    19081 atggatcaaa gttcgctccc gtttgttgga acccgccgtc tttaattcac ttagcggccg
    19141 tggcggacgg atagtcagtt catcgggtgc aacaaaatct agtggagaat gacgaatgac
    19201 atcatccgga gagtatccag tcatctgata aattgccgag tttgccatga tgatttggtt
    19261 attctgatcc acgatcagga ttccgtcagg agccgcctcg aacaacattc gatagtgaga
    19321 ctcactaatg aaagcgcgct gaatagcatt gtggaggctc ttgagtgtga tccccaacac
    19381 aaatgcagcg gatatggaaa aaacagcatt ggcagaccaa atcgtgaact ccgtttggat
    19441 tacgccccgt gggggaagca gaccttcatt agaggcatag atcattatta atccgctggc
    19501 aacgtttaat agtgcgaaga ccacagaagc gcgccaatta atgatcaaac cagcaacgat
    19561 gacaatcaga acctgtccat aaagactggt gccgcgaatt ccatatccat ctgcgattgc
    19621 ggcgatcgtt atgttcaacc aggaaagaga gacgaacagc acactggcaa ggtacacttt
    19681 tccggatcgc atcaaagccc acaaaactgc catgaacaat ataggaaata gcgaaaaagc
    19741 aatgcttttg atctcgctgc gggcgacgct cagaataaaa acactataga acagtgcccc
    19801 tccaacaccg atcatcaata tggcgttgag tgccattgcg acgcgggttt tatcctcatc
    19861 ttcagggaag accggagggc ggagccagtg aagaatgtca gataacaact taactctctt
    19921 cctaacacat ttgggaaact tgaatcgttt cggggagtca gaattggatt gaaatgccgt
    19981 taaaagtata tcacccacaa acttacagca aaagcttaca gttgaggtcc gtcttattgt
    20041 cggcttctaa agcccagctt ccatgggcta gaaaaataac gagacccgga tttgcctttg
    20101 gttatgcatc tatcttctcg taagatgcga tgatgcgatc ccacaggccg ttcatgttgt
    20161 cgtcgatggt ctggaattcc ggatgcgctt gaaacgagtc caatgatcga cgcaagcctt
    20221 cagcccagtt cactcgacaa ttaaagtcag ggacgaaacg cttgatcttg ctattatcga
    20281 ataccacgct gttgacctta tcacccagca gggtgcctaa tttttcaggg tcgtgctgaa
    20341 tgatcatctc cgatgagacg tgcaccagat tcggttccaa ccctaatgcc tgatacactt
    20401 cgagaaaaat ttgattccag gtgagaactt catctgaagt gatatggaat gcttcaccaa
    20461 ttgcattcgg attccccagt aagcccatca accctgtggc aaaatctgca ttccaagtga
    20521 gtacccatag ggaagtccca tcgccgggga caatgacctt cctgccgcgc tttatccggt
    20581 caacaacagt gtacggatga gaccaactcg caccaacgag cgggatttga gaagttccgt
    20641 atgtcaacga gggacgaatg attgtgaccg gaaatccttg ttctctgtac tctttcatta
    20701 gtcgttcttc gcaggcaatc ttattgcgcg aatacagcca atacggattt tccagtggtg
    20761 tttcttcggt aatcaggtag tgcttcgggg gcttttgata ggcagaggcc gaactaataa
    20821 aaacaaactg atcagttttc ccctgaaata accggagatc acgttcaacg tcagcgactg
    20881 taaaagcgag gaaatccaca acggcatcaa atcggtgacc tgctaccagg gaggcaagat
    20941 tggcttcttc cgtgtagacg tcagcattta agacgattgc tccttctggt acagcatagt
    21001 gggcggaagc agagcgattc acgataacta actcgtggcc acgcgcaacg gccaggtcag
    21061 aacaagccga actgattaag ccggtaccac cgatgattag aattttcatc ctccctcagc
    21121 tctacctttt tcccaattcc gggagagggg tggcaatcat tcgattagca aggtagttct
    21181 ctacccagaa gataatcagg gcgagccaga cgatactgaa accgatcaag cgcgactgat
    21241 cgaatgcttc cttatagatg aaaatgccga tgagaaattg aatcgtcggc gcgagatatt
    21301 gcaatacacc g
//
LOCUS       DASTJP010000030         5129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_730610, whole genome shotgun sequence.
ACCESSION   DASTJP010000030 DASTJP010000000
VERSION     DASTJP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730610"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..1808
                     /locus_tag="VFQ23_00865"
     CDS             435..1808
                     /locus_tag="VFQ23_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HET9905149.1"
                     /translation="MSDQTRFILNESDLPKFWYNINADSPVPPTPVLHPGTLEPVTPD
                     FLSVLFPMDLILQEISTEQYIEIPEEVREIYRLYRPTPLLRARRLEKALDTTAHIYYK
                     YEGASPAGSHKPNTAIPQAFYNKRAGTKALTTETGAGQWGSALSMACSFFDLDLEVYM
                     VKVSYNQKPYRRILMETFGAQVYASPTDRTNYGRALLAENPDNPGSLGIAISEAVEAA
                     ATSKGAKKYSLGSVLHHVLTHQTVIGEESLKQMDMADEYPDVVISCVGGGSNFAGIAY
                     PFIRENLKNGKRTRLLAVEPTAAPSLTKGVYTFDYGDTARMAPIVKMHTLGHDFVPPA
                     IHAGGLRYHGMAPGICALYDAGHIEAVSVKQKATFEAAIQFARAEGIVPAPESAHGIR
                     AAIDEALDAKAKGEKRVILFNLSGHGHFDLGAYESYLTGKLEDYDYPEEAVKEAMAKL
                     PEVSFPG"
     gene            complement(1819..2142)
                     /locus_tag="VFQ23_00870"
     CDS             complement(1819..2142)
                     /locus_tag="VFQ23_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905150.1"
                     /translation="MIQVELRIPNNSKNKLIIETVENVCAANGLACALKGTLGSYPGC
                     VHWHYKKGKQKGTLEITWWERQNLLWFKVAENRRGKWMDDALTKLKKEIEVSLNKDPK
                     ERQVP"
     gene            complement(2165..3319)
                     /locus_tag="VFQ23_00875"
     CDS             complement(2165..3319)
                     /locus_tag="VFQ23_00875"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HET9905151.1"
                     /translation="MALTVSIGAAQALNGREAGLQATHHALNRLGSSTPVFSFVISSY
                     QYQAREVVSGVSGLLGDTPMIGFSSSAGLSSNGVHHNTVLVALLAGDLHAETRWMPGY
                     AQSGRETGLQLSKQASEQVDPRALFFFADGFNGDADQLCSSISNVSFPLVGALSSGDL
                     HTGHSYQIAGPQTGTGALSAAFLRGDLRVGVGAAHGWNPVGSQFRVTRSRGFWLRTLD
                     GRPASETYAQLFGYPARDWAFPPLSHLARLYPLGVEQGDQLVIRAPIRVEADGSFRMN
                     APVRDGVDAYLLVGSRFSCENAAQQAAQQALQQLDGKKPMFALVLVDIAWQMLLRSHP
                     GAEITAVQDILGKDVPILGGYTLGQIVPAKDSGSPQLLNQHIVVVAFAEA"
     gene            complement(3324..>5129)
                     /locus_tag="VFQ23_00880"
     CDS             complement(3324..>5129)
                     /locus_tag="VFQ23_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905152.1"
                     /translation="AQLDLLQAIADQTVGAIVKARLLQETENRARQLATLNEITRQLT
                     STLEQEPLLHNILENAVNILNCEAGTLFLMDEQTDDLIFRVTVGPVATNLLGQRLPAG
                     AGIVGRAVQLRAPVIENDAQNSTSHSVGTDRQTGFISRSLLAVPMQIKDRVLGVIEVI
                     NRKDGLPFVEDDQNLLTAFAGQAAVAIENARLLSLIDQELSERVEELQVMGRITRELN
                     ASLEVDRAMRTTLEWAMRRSHAEAGLIGMIEEDKLRLMAQQGYDELLTDDGDLRLPLE
                     LPAIQSAIESGQPDQVSLINNGAKGILPSAHTQIIVPIRREGQVIGLILLESNSDSQG
                     NLSFLNNLSDSAAIAIANAQLYGEVQKANLAKSDFVSFVAHELKNPMTSIKGYTELLA
                     AGSVGQINEMQTNFLSTIRANVERMSALVSDLNDNAKIEVGRLRLDYKPVDVPDIVDE
                     VVRSTKRQVDDKKQDVELRLPPELPQVWADRTRVGQVLTNLVSNAHKYTPEGGRILVG
                     AETAGNQWDPEGARQVVHLWVKDDGIGISIEDQAKIFQQFFRSDDSKAREAPGTGLGL
                     HITKSLVEMQGGRIWFDSEFRKGTTFHFTVPVAEG"
BASE COUNT         1293 a         1363 c         1232 g         1241 t
ORIGIN      
        1 agagagcagg tgaatcagtg aaagcctgcc cgaaacgaca ctgctgaatg gacctcagag
       61 ccgcaagagc aaaagtcttc tattgggtta ttcagacaca agtagctcaa gccgggaaga
      121 gcacccgtta tcacagcaga gtcacgtagc gactggaaga cgataaacta gtcgatgaac
      181 tgactacgta gagtgaggca gctttccttc aatgaaagca ggcctaactt gggtggcacc
      241 acggacgcca aagcagaaca gcgttcgtcc catgatggga tgaacgctgt ttttttgttt
      301 tgacctcacc ccaaccctct cctaaaggag agggagttaa ggagaaatat ggttgacatc
      361 atttttcatc caccttgaag aggatagcaa tacacagttt gccattgagt tatttattca
      421 aaaaggagaa gatcatgtct gaccaaaccc gtttcatcct gaatgaaagt gacctgccga
      481 aattctggta caacatcaat gccgatagtc cagttccacc aacgcccgtc ctgcatcccg
      541 gcaccctgga gcctgtcact ccagatttct tgtcggtact tttccctatg gatctgatcc
      601 tgcaggaaat cagtacggaa caatatatcg aaatccctga agaagtacgc gagatctatc
      661 ggctctatcg tccgacacct ttattacgtg cgcgacgcct ggagaaagca ctcgatacga
      721 ctgcccatat ttactacaaa tatgagggcg cttcgcctgc cgggagccat aagcccaaca
      781 cagcaatccc gcaggctttc tataataaaa gggccggcac aaaagcgtta actactgaaa
      841 cgggcgcggg gcaatgggga tcggcccttt cgatggcttg tagtttcttt gacctggacc
      901 ttgaagttta tatggtcaaa gtttcataca atcaaaaacc ctatcgccgc attcttatgg
      961 agacctttgg agcacaggtc tatgccagcc caaccgatcg cacaaactac ggccgcgctc
     1021 ttttggctga gaacccagat aaccccgggt ctcttggcat tgccatctct gaggctgtcg
     1081 aagccgccgc gacgtctaaa ggtgcaaaga aatatagtct tggttctgtg ctccatcatg
     1141 ttctgaccca tcagacagtg attggtgaag agtctctcaa gcaaatggac atggctgacg
     1201 aatacccgga tgttgtgatc agctgtgttg gtggtggctc gaacttcgca ggtattgcct
     1261 atcccttcat acgtgaaaat ttgaaaaacg gaaagcgcac gcgtttgctt gccgtggagc
     1321 ccactgccgc tccctccttg accaagggcg tctacacgtt cgattatggt gatactgctc
     1381 gaatggctcc aatcgttaag atgcacacac tcggacacga cttcgtccca ccagccattc
     1441 atgccggtgg acttcgctat cacggcatgg cgcctggcat ttgcgcgtta tacgatgcag
     1501 gccatattga ggcggtgtct gttaaacaga aagccacctt cgaagccgcc atacaatttg
     1561 cacgcgccga aggaatcgtt cccgcacccg aatccgcaca tgggatacgc gccgcaattg
     1621 atgaagcgct ggatgcgaaa gcgaaaggtg agaaacgcgt gatccttttc aatctttcag
     1681 gtcacggaca ttttgatctt ggtgcgtatg aatcgtatct aacgggaaaa cttgaggatt
     1741 atgattatcc cgaggaagca gtaaaagaag cgatggcgaa gttaccagaa gtgtcgtttc
     1801 ctgggtaaaa atcagaccct aagggacttg tcgttcctta gggtccttat taagagaaac
     1861 ctctatttcc tttttcagtt tcgttaaagc atcatccatc cacttccctc ttctattttc
     1921 tgccaccttg aaccataaaa ggttctgacg ttcccaccat gtgatttcca gcgtcccttt
     1981 ttgtttgcct ttcttatagt gccaatggac acagccagga taacttccaa gcgtgccttt
     2041 caaggcgcaa gcaagaccat tcgccgcgca gacattttca acagtctcaa tgattaactt
     2101 gttttttgag ttattgggaa tccgaagttc cacctgaatc atttgtcaat gaataatgga
     2161 actgttacgc ttctgcaaag gcaacaacga caatatgctg gttcaacaat tgcggtgacc
     2221 ccgaatcttt ggcagggacg atctgcccta acgtatatcc accaagaatg gggacatctt
     2281 ttcccagaat atcctgaact gctgtgatct cagcacccgg atgtgacctc agtagcattt
     2341 gccatgctat atctaccagc accagggcaa acatgggctt cttgccatca agttgttgta
     2401 aagcttgttg cgcagcctgc tgtgcggcat tctcacagga aaagcggctt ccgaccagga
     2461 gatatgcatc cacgccatcg cggacggggg cattcatgcg gaagctgccg tcggcttcca
     2521 cgcggattgg tgcgcggatg accagttgat caccctgttc cactccaaga ggatacaggc
     2581 gcgccagatg gctgagcgga ggaaaggccc aatcacgggc cggatagcca aagagttgag
     2641 catatgtttc ggaggcggga cgaccgtcta acgtccgcag ccaaaagcct cgcgaacgcg
     2701 tcacgcggaa ctgactgcca accgggttcc atccatgggc agcgcctact ccgacgcgca
     2761 ggtcaccgcg tagaaacgct gcacttagcg cgccggtccc cgtctgtggg ccagcaatct
     2821 ggtacgaatg accggtatgc aaatctcccg aagaaagcgc gccaactaat ggaaaggaga
     2881 catttgagat tgaactgcac aattgatccg catccccatt gaaaccatcg gcgaaaaaga
     2941 agagcgcgcg agggtctacc tgttcagaag cctgcttcga aagctgtaaa ccggtctctc
     3001 gaccgctctg tgcatacccc ggcatccagc gcgtttcggc gtgcaaatcg ccggccaaca
     3061 acgccaccaa aacagtattg tgatgtacgc cattggaact taagcctgcc gaactgctaa
     3121 agccgatcat gggagtatca cccagcaatc ccgaaacgcc actgaccact tctctggcct
     3181 gatattgata cgaggaaatc acaaagctaa aaactggcgt ggaggaacca agccgattca
     3241 acgcatggtg agttgcctgc aaacctgctt cgcgtccatt cagtgcttgt gccgcgccaa
     3301 tggaaaccgt aagagccatc taattatcct tccgccactg gaacggtaaa gtggaacgtt
     3361 gtaccttttc tgaattcact atcaaaccag atgcgaccac cctgcatttc aaccagactt
     3421 ttcgtgatgt gcaaacctaa gcctgttccc ggtgcctctc gtgcttttga atcatccgag
     3481 cggaagaact gctggaaaat ctttgcctgg tcctcaatac taatgccgat cccatcgtcc
     3541 ttcacccata agtggacgac ttgtcttgca ccctcagggt cccattgatt cccggcggtt
     3601 tccgcgccga caagaatcct tccaccttct ggtgtgtatt tgtgcgcatt gctaaccaga
     3661 tttgtaagaa cttgccccac acgagtacgg tctgcccaaa cttgaggcaa ttcaggtggc
     3721 aggcgaagct ccacatcctg ctttttgtcg tctacctgcc gctttgtgga acgtacaact
     3781 tcatcaacaa tatcgggtac gtccaccggc ttatagtcaa ggcgcaggcg tcccacttcg
     3841 atcttggcat tatcgttgag atctgaaaca agagcagaca tgcgttccac attggcgcgg
     3901 attgtactga ggaaattggt ctgcatttca ttaatttgtc caacagaccc agccgccagc
     3961 aattctgtat aacccttaat agaagtcatc gggttcttta actcgtgcgc aacgaaggag
     4021 acgaaatcac tctttgcaag gttagccttt tgcacctcac catatagctg cgcgttcgca
     4081 atcgcgatcg cggcgctgtc gctcagatta ttcagaaatg acaaattgcc ttgtgagtca
     4141 ctgttgcttt cgagcaaaat taaaccaatc acctgtcctt cacgtcgaat gggaacgatg
     4201 atttgcgtat gtgcagaggg aaggattccc ttcgcaccgt tattaataag ggatacttga
     4261 tcgggctgtc cactttcaat cgcagactga atggcaggta attccaaagg taatcgtaga
     4321 tctccatcgt cggttagcaa ctcgtcatag ccttgttgtg ccatcaggcg aagtttatct
     4381 tcctcgatca tgccgatcag gcctgcttct gcatgtgacc ttcgcatcgc ccattcaagt
     4441 gtcgtgcgca tggcacgatc cacttcaagg ctggcattca attcgcgagt gatccgcccc
     4501 ataacttgta actcttccac cctctcagaa agctcctgat caatgagaga caaaagacga
     4561 gcgttttcaa tggcaactgc agcctgacct gcaaaggcgg tgagcaggtt ttgatcatct
     4621 tccacaaatg gaagcccatc tttacgattg atgacttcga tcacgcccag cacacgatcc
     4681 ttgatctgca tcggaacagc aagaagagag cgtgagataa atcctgtttg tctgtctgtg
     4741 ccaactgaat gagaagtcga attttgtgca tcattctcaa tgacaggagc acgcaattgc
     4801 accgcacgcc ccacgatgcc agcacccgcc gggagacgtt gaccgagcag gttggtggct
     4861 accgggccga ccgtcacacg gaagatcagg tcgtcagttt gttcgtccat caggaacaac
     4921 gtgccagcct cgcaatttag gatgtttacc gcattctcaa gaatgttatg aagcagaggt
     4981 tcctgttcca gggtggaggt cagttgacgc gtgatctcgt tcagcgtggc gagttgtctg
     5041 gcacgatttt ccgtctcctg aagaaggcga gctttgacga tcgcgcctac cgtttgatct
     5101 gcgatagctt gtagaagatc cagttgtgc
//
LOCUS       DASTJP010000031         6388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1049148, whole genome shotgun sequence.
ACCESSION   DASTJP010000031 DASTJP010000000
VERSION     DASTJP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049148"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="VFQ23_00885"
     CDS             <1..1581
                     /locus_tag="VFQ23_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9905153.1"
                     /translation="IQQLLEQILGYESIQLFEERARLVQENFALTMENASFIAQICQR
                     LDGIPLAIELAAARVDMFSTEQIAARLDESFKLLTGGGRTTLPRHQTLRASIDWSWDL
                     LSEPERILLRRLSVFAGGWTLEAAEAVCPGFGIELQQMLDLMTHLMTKSLIVSKQDDE
                     RGIRYHLLEMIRQYAHEKLQEAGEVDAIRDKHLGWVLAWAEQIQPEFNGRNRAASLAN
                     ADLERDNIRLAIDWGLRMGQFERSMKICIALRSYLDGRIPYGETRRWLDLGLSHRDHF
                     SKSTLARIYTEAAWVTLRQNDLLHGIDYARESLKMAEELNDTQVVAEAMRSLGVVEIF
                     AGNYAEATTYFEKVVALYQALGNDHGAFVAIGNLALSLFYQGKVEDAIALLAKYMDSV
                     MSLGNQDRIAWFHFALGLFKLVHGDVEKALPNLKSGLSLFHEIKEINFTAVCMMAVAF
                     LASARGNAVRAARLFGAGEAIHESIGTVLDPTAQSLRGTYVEKTRAMLDESAFASAWA
                     EGRAMSLDEAVVYALDDK"
     gene            complement(1589..3490)
                     /locus_tag="VFQ23_00890"
     CDS             complement(1589..3490)
                     /locus_tag="VFQ23_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905154.1"
                     /translation="MKKIFITYIILTLFLSSCGTLEISFATPVAESASEPIAPPITQE
                     SKLSLESSSEEIQRAMLESATKWKSIWMDGTITQYAPDGGDTPINVTREQVWIDLTTS
                     RFRILTGPADGAAEKFKASDGMTILEIDLKSGQSQSSPLPDFAKAGQFVPHLQPGVAS
                     PQPLWGQMGSGLSQLAFPSDLAQNEGTFKPVAIEIIADRETLVVDWTYIQNEAPSWRL
                     WLDTKTAVILKKQSYAKEGGGPIQEESVVGKVRYDDIFPDSLFGVPASLPDFENGAGQ
                     GSEPVETATSLSSGKDALGELYFFTLPHQSNPSAQLVRLPGLCVVGAVECPQLETVQV
                     HFGLNFTLSELRWSPAGNFAAFAYPDHPNGTPYKLWSFDPTASMWTPIFEYAYIDPPI
                     WSSDGQWLAFRVQDGLGGEDVYVIQPDGSGLKNLTASDDLPAQGRPYVTYGWVGQNII
                     VRSANLGTEGSVYLIRVADGHVQSMFDTQLTKSVFVPSSDGAQLAYDEYNSESQVHSL
                     RVAEPDGANVLELATFTGGTLYPIVWSPDNRQLAFAYYTEATQSTPIADVHVISLDGT
                     GSKQVYRGVTIGSILFSPDGNYLLINETTSPTGGHLFVVNLETLEQRIIQAPGLSLNS
                     DWVMPSWRR"
     gene            complement(3691..4305)
                     /locus_tag="VFQ23_00895"
     CDS             complement(3691..4305)
                     /locus_tag="VFQ23_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9905155.1"
                     /translation="MNEDLLIQSAQRGDLDAFNQLILRYQNLLFSIALRILADEDSAS
                     DALQEALLSAFRKIHMFRGGSLRSWLARIVINACYDELRRKRRQPSLPFEQMDADGEE
                     IEPDYWLTDSSVGLEEQYESTELERAIQKCLSSLPPDYRTVFVLVDVECMSYEEAAEA
                     VHVPIGTVKSRLARARMQMRKALQSFGDLLPLAYRMGTPLSMNV"
     gene            complement(4459..5385)
                     /locus_tag="VFQ23_00900"
     CDS             complement(4459..5385)
                     /locus_tag="VFQ23_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9905156.1"
                     /translation="MFNRRWIFFLLFLLPLLIGVLASFGMQVMFNPVPILLFRIDIGM
                     VTFLAGLFISVVLLSFLLGVISNERRANKLIDDSLRESEQGRQRFLRRLDHEIKNPLT
                     GLRAALVNLQESGDTTERQRASEHAGRAVERLTRLLTDLRKLSDLEERSIERRPVDVP
                     DVLEDVVDAARSLPAYQGRNINLFISKVPSLPAVTGDRDLLVLAVYNLVENALKFTSA
                     ADSLEVRALEDGKAIVIEVADSGTGISSEDQSKIFEELYRGSNARSTEGSGLGLALVN
                     RIIALHGGAVTVRSNQSEPRGTVFTVRLPVKR"
     gene            complement(5375..>6388)
                     /locus_tag="VFQ23_00905"
     CDS             complement(5375..>6388)
                     /locus_tag="VFQ23_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9905157.1"
                     /translation="HMSRRRRLYLVLLSLPVWVGLLSAFLANWLLRPNLSFLYPTEFG
                     IAAFVFGGVIREPRIVAFVTGFLITSFLVLLYFWDQRSARQTQILLTEAQREAERSRR
                     SFLRRLDHEIKNPLTGLRAALVNLQETQVTDERQHAAETAGRAVERLTRLLTDLRKLS
                     DLGERPIELSIVDIPDLLQDAVDAAQVLPAYDRREISLLIPKVPSPFPFVTGDRDLLV
                     LAVYNLIENALKFTSTEDSVEVRAAEDGRNILIEVADSGVGVPPEDAPKIFEELFRAS
                     NARGTEGSGLGLALVHRIVSLHDGKISVRSSQENPRGTVFTVRLPVMKRSKVAAQNNS
                     NVQ"
BASE COUNT         1636 a         1606 c         1636 g         1510 t
ORIGIN      
        1 attcagcaac ttctcgaaca gatattagga tacgaatcga tccaattatt cgaagagcgc
       61 gcccgtctcg ttcaggaaaa tttcgccctg acgatggaga acgcttcttt cattgcccag
      121 atctgtcaac gtctcgacgg cattccactg gcaattgagt tggctgccgc gcgcgtggat
      181 atgttttcaa cggagcaaat cgccgcgcgg ctggatgaaa gtttcaaact gctcacaggc
      241 gggggacgca ccacattgcc gcgtcatcag accttgcgcg cctccattga ctggagttgg
      301 gacctgctct ccgaaccaga gcggattctc ctacggcggc tctccgtttt cgccggtggc
      361 tggactctgg aagcggcgga ggcagtctgc ccgggattcg ggatcgaact ccagcagatg
      421 ttggacttga tgactcacct catgacaaaa tctctgattg tgtcgaaaca ggatgacgaa
      481 cgcggaatac gctatcatct gctcgaaatg attcgacaat acgcgcacga aaaacttcaa
      541 gaagcggggg aggtggatgc catccgggat aagcatcttg gctgggtgct tgcttgggct
      601 gagcaaatcc agccggaatt taatggtcga aatcgagcag cgagtctcgc taatgccgat
      661 ctggaacgag ataatatccg cctggcaatt gattggggtt tgagaatggg gcagttcgaa
      721 cgcagtatga aaatatgcat cgccttacga tcttatttgg atggtcgcat tccctatggt
      781 gaaacacgcc gatggttaga cttgggttta tcacaccgcg atcatttttc aaaaagcaca
      841 ttggcgagga tttacaccga ggcggcgtgg gtcactttac gacaaaacga tctgctacat
      901 ggaattgatt acgcaagaga aagcctgaaa atggctgagg aattgaatga tacacaggtt
      961 gttgcagaag caatgcgcag tctcggggtg gtcgagatat tcgctggcaa ctatgccgag
     1021 gcgacaacat atttcgaaaa agtcgtggct ttatatcaag cgctgggaaa tgatcatggg
     1081 gcttttgttg caatcgggaa tctggcgctt tcgctctttt atcaaggaaa agtggaagat
     1141 gcgatcgctc tacttgcgaa gtatatggat agtgttatgt ctcttggtaa tcaggataga
     1201 attgcctggt ttcatttcgc cttggggttg ttcaaattgg tgcatggtga cgtcgaaaag
     1261 gcgctcccca atttgaaaag tggtttatcc ctttttcacg aaataaaaga aattaacttt
     1321 actgctgttt gcatgatggc tgttgctttt ttggcaagcg cgcgtggaaa tgcggtaagg
     1381 gcggcgcggt tgttcggggc aggtgaagcg attcacgaat cgatcggtac tgtgcttgat
     1441 ccgacagccc aatcgctccg aggcacatat gttgaaaaga cccgcgccat gcttgatgaa
     1501 tcggcttttg cctccgcctg ggcagagggc cgcgcgatga gtctcgacga agcagttgtg
     1561 tatgcacttg acgacaaata gggataatct agcgtctcca cgaaggcatg acccaatcgg
     1621 agttcagaga taagccaggg gcctgaataa tgcgttgttc taatgtttca aggttcacaa
     1681 cgaagagatg tccaccggtt gggctggtgg tctcgttaat cagtaaatag tttccatccg
     1741 gtgaaaatag aatcgaacca atggtcacac cgcgatagac ctgctttgaa ccggtgccat
     1801 caagactaat gacgtgtaca tccgcaattg gtgtgctttg tgtggcttcc gtatagtaag
     1861 caaaagcgag ctgacgatta tccggtgacc agacaattgg atacaaggta ccacccgtaa
     1921 aagtggctag ttctaaaaca tttgcgccat caggctcagc aacgcgcagc gaatggacct
     1981 ggctttcaga gttgtattca tcataggcca gttgagcgcc atcactcgat ggaacgaaca
     2041 cggatttcgt aagttgtgtg tcaaacatcg actgaacgtg tccatctgca acgcggatca
     2101 gatataccga gccttcagtt cctaaattcg cggaccgaac gataatattc tgcccaaccc
     2161 aaccgtacgt gacatagggt cttccttggg caggcagatc atcggaggca gtcagattct
     2221 tcaggcccga gccatctggt tggatcacat acacatcttc tccacctaaa ccatcctgaa
     2281 cgcgaaacgc cagccactgt ccgtctgaag accagatcgg tgggtcaata tacgcatatt
     2341 caaatatagg cgtccacatg ctggcagtcg gatcgaagga ccacaatttg tatggggttc
     2401 cattcgggtg atctggatat gcaaacgcgg caaaatttcc cgctggtgac caacgcaact
     2461 cgctcaacgt aaaatttaat ccaaagtgaa cctggacggt ttccaactgc gggcattcca
     2521 ccgcgccgac gacacataac cccggcagtc gtacaagttg cgcagatgga ttcgattgat
     2581 gcggcagcgt gaagaagtac aactcaccca gcgcgtcttt tccggaagat aggctggtgg
     2641 ccgtttcaac aggttcactt ccctggccag cgccattctc aaaatcaggc agggatgcag
     2701 gaacgccaaa caaagaatcc ggaaatatgt catcatagcg gactttacca acgacacttt
     2761 cctcttgaat gggtccgcca ccctcttttg cataactttg tttcttcaat atgacggccg
     2821 tctttgtatc caaccacagt cgccaggaag gtgcttcatt ttgaatgtag gtccagtcaa
     2881 caacaagcgt ttccctgtct gcgatgattt caatcgcgac tggtttgaat gtgccttcat
     2941 tttgtgcaag atctgaagga aaggcaagtt gcgaaagacc agatcccatt tgcccccaca
     3001 gaggctgcgg agaagcgaca cccggctgca agtgtgggac gaattggccc gcttttgcaa
     3061 aatcaggtaa aggcgaagac tggctttgac cgctcttcaa gtcgatttca agaatcgtca
     3121 tgccatcact ggctttgaat ttttccgccg ccccatccgc aggtccggtt agaatgcgga
     3181 agcggctggt ggtgaggtcg atccaaactt gttcgcgtgt tacatttatt ggagtatctc
     3241 caccgtccgg cgcgtactga gtaatcgtgc catccatcca aatggatttc catttcgtcg
     3301 cgctttcaag catggctcgt tgaatttctt cagacgaaga ctctaatgaa agttttgatt
     3361 cctgggttat cggtggagcg atgggttctg aggcactttc agcaacgggc gttgcaaagg
     3421 atatttccaa cgtgccgcaa gatgagagaa agagcgttag gataatgtat gtaataaata
     3481 ttttcttcat agccacttta tttttgttat agaaggaatt cgttggtcga gtagagctgg
     3541 gtgctagtcc cagctggtat cgagaccaac aagtatcaaa aaaatacttg aaacaatagt
     3601 cactttgcca attggtagtc tcgacacttg cgccaggagc aagtgcaggt gtacgtggcg
     3661 ccaacgagcg ccactactca accaccggta ttacacattc atcgaaagcg gggtgcccat
     3721 ccggtaagca agtggcagta aatcgccaaa ggattgtaat gctttgcgca tctgcatgcg
     3781 tgcgcgtgcc agacggctct tgaccgtacc gatcggaaca tggaccgctt ctgcagcttc
     3841 ctcataggac atgcattcca cgtccaccaa aacaaaaacc gttcgatagt ctgggggaag
     3901 ggaggacagg catttttgaa tcgctctctc cagttcagtc gattcatatt gctcttccag
     3961 cccaaccgag gaatccgtta gccaataatc gggttcgatc tcttcgccat cggcatccat
     4021 ctgctcaaaa ggcaaggaag gctgacggcg tttgcgcctg agctcatcat agcaggcatt
     4081 gatcacgatg cgcgccagcc agcttcgcaa tgaaccgccg cgaaacatgt ggatctttcg
     4141 aaaggctgaa agtaacgctt cttgtaacgc atccgatgcg ctgtcttcat ccgccaggat
     4201 tcgcagggcg atgctaaaca atagattctg gtaacgcaga attagctggt tgaaggcatc
     4261 caggtcgccg cgctgtgcag attgtattaa gaggtcttcg ttcatggctg tgtcatccaa
     4321 tcttgtaaaa cggataaaaa atgcagagaa atatgatgtg ctgctttcgt atcctattta
     4381 tactccccgg gtgttacaag cttgtctcag agatgtcaca gttctgtaac cccctgttac
     4441 aaaaatttgg gactttttct atcgttttac aggcaatcgc acggtaaaaa ccgtcccgcg
     4501 cggctcactc tgattggaac ggacggtcac ggctcccccg tgcaaggcga taattcggtt
     4561 gaccagcgcg agccccagcc cactcccctc ggtgctacgg gcattggaac cacgatagag
     4621 ttcttcaaaa atcttggatt gatcttcgga tgaaatcccc gtgcctgaat ctgccacttc
     4681 aatgacgatg gctttcccat cttccagggc acggacctcc agcgaatcag ccgcagaggt
     4741 gaacttcagc gcgttttcaa cgaggttata tactgccagg acgagcagat cgcggtcccc
     4801 cgtcaccgca ggcagtgacg gtaccttgct gataaacaga ttgatattcc gcccctgata
     4861 cgcaggcagg gaacgtgcag catccaccac atcttccaat acatcgggca cgtcaaccgg
     4921 tcgccgttca atggagcgct cttccagatc ggaaagttta cgcagatcgg tcaataagcg
     4981 cgtgagacgc tccacggcgc gacccgcatg ttcagaggcg cgctgacgct cggtcgtatc
     5041 gccagactct tgcaaattga cgagcgcggc gcgcaagcca gtcagcggat tcttgatctc
     5101 atgatcgagc cggcgcaaaa agcgctggcg tccctgctca gactcgcgca atgaatcatc
     5161 gatcaattta ttggcgcgtc gttcattgct gatcacgccc aggagaaaag acaacaaaac
     5221 aaccgaaatg aatagtccgg ccagaaacgt caccataccg atatcaattc gaaaaagaag
     5281 aatcggcact gggttgaaca ttacttgcat gccaaaggaa gccaacacac caatgagtag
     5341 cggaagcaaa aaaaggagaa agaaaatcca gcgtctattg aacattcgaa ttattttgag
     5401 cagcaacctt gctgcgcttc atgaccggca aacgcacggt gaacacggtt ccacgagggt
     5461 tttcctggct gctgcgcaca ctaatctttc catcatgcag tgagacgatt cggtggacca
     5521 atgccaaacc caacccgctg ccctccgtcc ctcgcgcgtt ggaggcgcgg aagagttctt
     5581 cgaagatttt cggcgcatcc tctggtggaa ctccgacgcc cgaatccgca acttcgatga
     5641 ggatgtttct tccatcctct gcggcgcgca cttccactga gtcctctgtc gacgtgaact
     5701 tgagtgcatt ttcgatcaga ttataaacag ccagcaccag caggtcgcga tcaccggtca
     5761 caaacgggaa aggagaaggc actttgggga tcagcaggct gatctcacgc cgatcatagg
     5821 cgggcagcac ttgcgcggca tccactgcat cctgcaatag atcagggata tccacgatgg
     5881 agagttcaat tgggcgttct cccagatctg aaagttttct caaatcggta agtaaacgag
     5941 tcaatcgctc aacggcgcgg ccagcagttt cggcggcatg ttgacgctca tcggtgactt
     6001 gtgtttcctg cagattgacc agcgccgcgc gtaagcctgt gagcggattc ttgatttcgt
     6061 gatcaagccg gcggagaaag cttcgtcgac ttcgttccgc ttcacgctgg gcttccgtca
     6121 ataagatttg ggtttgccgg gcactgcgct gatcccaaaa ataaagaagg accaaaaagg
     6181 atgtaatgag aaaaccagta acaaaagcga cgatacgcgg ctctctgata accccgccga
     6241 aaacaaacgc tgcaattcca aattctgtcg gataaagaaa tgataaatta ggccgcaaaa
     6301 gccaattcgc aaggaatgct gataacaatc ccacccagac cggtagcgaa agcaggacga
     6361 gatacaaccg ccttcttcga gacatgtg
//
LOCUS       DASTJP010000032         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_625849, whole genome shotgun sequence.
ACCESSION   DASTJP010000032 DASTJP010000000
VERSION     DASTJP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625849"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFQ23_00910"
     CDS             complement(<1..411)
                     /locus_tag="VFQ23_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9905158.1"
                     /translation="MMRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPG
                     GVIPQILEKLSVSPEALTERLDATLRASPKANIFGGGAGQIFITPRVKRIIDLANEEA
                     NRLKDEYISTEHIFLAILTERNTPAARILESAGLT"
     gene            complement(823..1098)
                     /locus_tag="VFQ23_00915"
     CDS             complement(823..1098)
                     /locus_tag="VFQ23_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905159.1"
                     /translation="MECWTCRSNTGEKRISPGPTIFEGKYWLIEHVYPVKRIGWIVLV
                     LKRHAEALHELTSEEFMELAQIQARLIPLLHEELHCEKEYLSCYAEM"
     gene            complement(1155..2591)
                     /locus_tag="VFQ23_00920"
     CDS             complement(1155..2591)
                     /locus_tag="VFQ23_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9905160.1"
                     /translation="MSHSVSVQNRRGTGWIVFGILMGLLIMVAVVYNAIRFFLDQNNY
                     NKGHQAYLQADCTVAIQHFDSVIEGWRMVDMGGYPGLARQEKAECIPFRAAVDRQQAG
                     DFSGALMAYMNFISEYANSALTEAARSRGSSLFVQARPSELADEQSCGSIDSLRGANL
                     IPQQEVNLPPFYLSCGQFYDSASNQQGSFQMYKNLLTEYPGDLLAETAAASLLTNPLA
                     CKESEALKNSVIAARNEFMPSLYYQCGQSYDSSGNEQSSFEMYQKLLTEYPGASLAEN
                     AAASLLANPLACKESDTLKNSVIAERNEFMPSLYYQCGQTYEGESNWDQAISMYEKFL
                     AAYPTHARAADAEAGLARAIVSQSQATSAGDIPTPERSGSTGSTVSEVVIQNDSPERL
                     RIVFSGPEARVEELEPCSSCTTYTGIGPLYCPEQGPIGRYPLTPGEYDVVVEAISGDG
                     TTPWTGNWSLVTGDEYYNCFFIITTFGP"
BASE COUNT          673 a          722 c          717 g          735 t
ORIGIN      
        1 cgtcagacca gccgattcca aaattcgggc tgcgggcgta ttccgctcgg tgaggatggc
       61 gaggaaaata tgttccgtgg agatgtattc gtccttgagc cggttggctt cctcgttggc
      121 taaatcaata attctcttca cgcgcggggt gatgaagatc tgtccggcgc cgccgccgaa
      181 gatattggcc ttggggctgg cgcgcaatgt cgcatcgagc cgctcggtca atgcctcggg
      241 gctgacgctc agtttttcca aaatttgggg gatcaccccg ccgggctgtt cgatcagcgc
      301 cagcaggatg tgttcggtat cgatctggtt gtggccgtag cgctggatga tctccgcggc
      361 gcgctgggcg gcttcctgtg ctctttctgt aaatctgtca aatcgcatca tagtgatagt
      421 tccttttctg ccgctctcat aaatgaactg attgtctgga ttctgcgtga tattcgattc
      481 gattatcaag ttagtaaaaa acgcggcacg tcgaaaaaca aaaaccccga aggtcgtcgc
      541 gtagcgcccc attcaggggg gcgagacctt tgggggcctt gtgacaaggc ggattataac
      601 aaaagcgcgt tggcaaattg tcaacgcgac gtaagaggag tattagactc caggcgtgta
      661 gccaaatttg tcccggagca gctcacagaa ggagatgatg tcgcctggca aaacagtttc
      721 ttccggcgcc accttgagca acgcaaaact ccggccgcct ttcaattcat ccggaaaatc
      781 cgcgggcctg gcgaagacat ggatatgaat gtgcttaaaa tgctacatct ccgcatagca
      841 tgaaagatat tccttttcac aatgcagttc ctcatgtaat aaaggaatca gcctggcttg
      901 aatttgagcc agctccataa attcttcaga tgtcaattcg tgcaatgcct ctgcatggcg
      961 tttcaaaaca aggactatcc atccgatcct ttttaccgga tagacatgtt cgatgagcca
     1021 atacttgcct tcaaaaatcg ttgggccggg tgaaattctt ttttcacctg tgtttgatcg
     1081 gcaggtccag cattccatgt tcacgttgca tcctgtatcc tgaacaatag ggcgagatcc
     1141 gggtctaatc cgtattaagg tccaaatgta gttatgataa agaagcaatt atagtactca
     1201 tcgccggtga ccaaactcca attcccggtc cagggtgttg ttccatcgcc actgattgcc
     1261 tctacaacaa catcatattc accgggcgta agtgggtaac gtccaatggg accctgttcg
     1321 gggcagtaca gcgggccaat cccggtatat gttgtacatg agctacacgg ctcaagttct
     1381 tcaacgcgtg cttccggtcc gctaaaaacg atacgcagtc tttccggcga atcattctgg
     1441 atcacaactt cactcactgt actgccggta cttccactgc gttctggcgt aggtatatcc
     1501 ccggcagatg tggcctggga ctgggacaca attgcgcgcg ccaggcctgc ttcggcatct
     1561 gccgcacgag catgggtcgg ataggcagca agaaatttct catacatgct gattgcctga
     1621 tcccagttcg actcgccttc gtaagtctgg ccgcactgat agtaaaggga aggcatgaat
     1681 tcattcctct ccgcgatgac actattctta agggtatcag attctttgca tgccaaagga
     1741 ttggccaata aggatgctgc tgcgttttca gcaagcgaag caccgggata ttcagtcagg
     1801 agtttctggt acatctcaaa ggagctttgc tcgttgcccg atgaatcata ggattggccg
     1861 cactgataat agagtgaagg cataaattca tttcgggcag caatcacact gttcttaagc
     1921 gcttcagact ctttgcacgc caaaggattt gtcagcaggg atgctgctgc tgtctccgcc
     1981 agtagatcgc cagggtattc cgtcaggagg tttttgtaca tctgaaacga gccctgctgg
     2041 ttgctggctg aatcatagaa ctgcccgcag gagaggtaaa aaggcggtaa attcacctct
     2101 tgttgcggaa tcaggtttgc cccgcgaagt gagtcgatgc tgccgcacga ctgttcgtca
     2161 gcgagttcgg atggcctggc ttgcacgaac agagaggaac ccctgctgcg ggctgcttct
     2221 gtcagtgcgc tattggcgta ttcgctaata aaattcatgt atgccatgag ggccccgctg
     2281 aaatctccgg cttgttgacg gtcaacggct gcgcggaagg gaatgcattc tgccttttcc
     2341 tgccgggcaa gcccgggata tccacccatg tcaaccattc gccacccctc gataacgctg
     2401 tcgaaatgct gaatggcgac cgtacaatct gcctgtagat atgcctggtg tcccttgttg
     2461 tagttattct gatccaggaa aaatcggatt gcattgtata ccacagcgac catgataagc
     2521 agtcccatca aaataccgaa aacgatccaa ccggtaccgc gtcgattttg tacagaaact
     2581 gagtgagaca tggcatttcc ctttcctacg catattttat ctttgcaacg ccgatctccc
     2641 aacaatcccc gaagagtact tggtacttta aggcgtttgt tcccgcttta ataaacgttg
     2701 gttccaggaa aattacggaa tgaacgaaca ccaggattca ctccatcaga tagactttgt
     2761 atacgcagca agaaatccag tctttccgct ttgtgttgcc ttgctccatg caagcttacg
     2821 cttcgcttgt tgattgcata agcagta
//
LOCUS       DASTJP010000033         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_729755, whole genome shotgun sequence.
ACCESSION   DASTJP010000033 DASTJP010000000
VERSION     DASTJP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729755"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFQ23_00925"
     CDS             complement(<1..341)
                     /locus_tag="VFQ23_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9905161.1"
                     /translation="MLSIIYGIASALSWGSGDFAGGLSSRKVGAYRAVFYADFFGFLL
                     VLLAFFIFPEPFPGTRVFWVSVIGGALGTAGLLTLYYSLSKGQMSIAAPVSALFAAIL
                     PVVVGSLTEGLP"
     gene            complement(416..952)
                     /locus_tag="VFQ23_00930"
     CDS             complement(416..952)
                     /locus_tag="VFQ23_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9905162.1"
                     /translation="MKWSTSPHRPPHLYVDDAWYFVTASTVDKVKILSSDEHFKLWVG
                     IFKSLIVEYRVKVVAWVVLPNHYHFLFLPQHGYDLGIFMKRLNGRTSYELNSLDNLRG
                     RSVWYSYWDTCIRGERDFWTRFNYIHYNPVKHGYVQKPEDWHFSSYRNHMRNDGSIWL
                     KAHLYDFPIHDLFDDDKF"
     gene            complement(1006..1890)
                     /locus_tag="VFQ23_00935"
     CDS             complement(1006..1890)
                     /locus_tag="VFQ23_00935"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET9905163.1"
                     /translation="MHSRRALLYMPGDDRRKIEKATTLGVDSICMDMEDGVAANKKAE
                     AREIIAEAMKELDFGGSERCIRINSVGSGLEKRDLAAALAAEPNAIVIPKIESPEQVK
                     WVSERIEIYELTSRLNVGSIRLLVGVETAKGILNLKEIAESDSRLEAIIFGAEDYAAS
                     IGATRTKDGIEVLYARSAVVTACAANDLQAIDMVYIDFRDTEGLRAESEQGAALGFSG
                     KQIIHPNQVVPVQEAFTPSDAAIEYAKRLVKTFKESEKEGKGAYALDGKMIDMPLLKN
                     AQKVLDRARAAGKLESGL"
     gene            complement(1996..>2395)
                     /locus_tag="VFQ23_00940"
     CDS             complement(1996..>2395)
                     /locus_tag="VFQ23_00940"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET9905164.1"
                     /translation="LGRTVTEMDNVLFSALTMNTQPLHMDQEFASKTEFGQRLVNGLY
                     TLGLVVGLTVSELTEGTIIANLGYDKVVHPNPVFHGDTIYAESEIIEKRESKSRPNVG
                     IVKIKCWGRKPDGTVVVEFERTAMFLKKTN"
BASE COUNT          614 a          646 c          526 g          609 t
ORIGIN      
        1 ggtagtcctt ccgtgagaga tcccacaact acaggcaaga tcgccgcaaa caaagccgaa
       61 actggtgcgg caatactcat ctgtcctttc gacaggctgt aatacaaggt gagcaaacct
      121 gctgtgccaa gcgccccgcc aatgacagat acccagaaga ctcttgtccc gggaaacggt
      181 tccgggaaga taaaaaaagc caggagcacc agtaaaaacc caaagaaatc ggcgtaaaaa
      241 acggcgcgat atgcaccaac ttttctgctg gagagaccgc ccgcaaagtc tcccgagccc
      301 cacgagagag cagaggcaat gccataaatt atggaaagca agttgaggct cctaaaggaa
      361 taattgggac gcggacttta gtccgcttga tacaacgatg acgccagtcc gctatttaga
      421 acttatcatc atcaaacaaa tcatgaatag ggaaatcgta gagatgcgct tttagccaaa
      481 tacttccatc attccgcata tggttgcggt aacttgagaa atgccaatct tcaggttttt
      541 gcacatatcc atgcttcaca ggattgtaat ggatgtaatt gaagcgtgtc cagaaatcac
      601 gctcgcctcg aatacaggta tcccaatagc tataccaaac gctccgccct cggagattgt
      661 caagcgaatt caattcataa gaagttcgcc cattcaagcg tttcataaaa atgccaagat
      721 catatccgtg ttgtggaaga aacaggaaat gatagtgatt tggaagcacg acccaggcta
      781 ccaccttcac tcggtattca acaatcaaac ttttgaaaat gcctacccac agtttgaaat
      841 gttcatcgga tgatagaatt tttaccttat caaccgtaga tgcggtcaca aaataccagg
      901 catcatccac atagagatgc ggcggacgat gaggggatgt tgaccatttc atagcttgcg
      961 actccggatc gcggacttta gtccgccgaa caaacgatag gcggactaaa gtccgctttc
     1021 cagtttcccc gcggctcgtg ctcgatccaa cactttttgc gcgttcttca acaatggcat
     1081 gtcaatcatc ttgccatcca gcgcgtaggc gcctttcccc tctttttcac tttctttaaa
     1141 cgttttgaca agtcgttttg catactcgat cgctgcatcg gagggagtga acgcttcctg
     1201 cacggggacg acttgatttg ggtggatgat ctgttttccg ctgaagccca gagccgctcc
     1261 ctgttctgac tcggcgcgca agccctcggt atcgcgaaaa tcaatataga ccatatcaat
     1321 cgcctgcaaa tcattcgccg cgcaggctgt gacaaccgcc gagcgcgcgt acaacacttc
     1381 gatcccatct tttgtacgcg tggctccaat cgatgcggcg taatcctctg ccccaaagat
     1441 aatggcttca agccggctat ctgactccgc gatttccttg agattgagta ttccttttgc
     1501 cgtttccaca ccgaccaaca aacggatact tccaacattc agccgactgg tgagttcata
     1561 gatctcaatg cgctcactga cccatttcac ctgttcgggt gattctattt tgggaataac
     1621 aatcgcattg ggttcggccg ccagtgcagc agccagatcg cgcttctcca accctgaccc
     1681 aacactgttg atacggatgc atcgctctga accaccaaaa tccaattcct tcattgcctc
     1741 tgcaatgatc tcgcgcgctt cagccttttt gttggctgcc acaccgtctt ccatatccat
     1801 acaaatggaa tccacgccaa gcgttgtggc tttttcgatt ttgcggcggt catcgccggg
     1861 catgtaaagt aacgcgcgtc gtgagtgcat agttttcctt ttaatatgtc attgtgatct
     1921 gcgaaaagca atctccggta catagtcaac gcgagattgc cacgacacgc ttcgcgtgtc
     1981 tcgcaatgac atttgttaat ttgtcttttt caaaaacatt gctgttcgtt caaactcaac
     2041 cacaacggtt ccatccggct tcctgcccca gcatttgatt ttcacaatcc ccacgttcgg
     2101 ccgtgacttt gactcgcgct tctcaatgat ttcactttcc gcataaatcg tatcgccatg
     2161 aaaaaccggg ttcggatgta caaccttatc atatccgagg ttggcgatga ttgtgccttc
     2221 ggttaattct gagactgtca gtccgacgac gagtcccagt gtatacaagc cattgaccag
     2281 cctctgacca aattccgttt tcgaggcgaa ttcctggtcc atatgcaggg gctgggtgtt
     2341 catcgtcagc gcagagaaga ggacgttatc catctctgtg accgtacgcc ccagt
//
LOCUS       DASTJP010000034         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_941914, whole genome shotgun sequence.
ACCESSION   DASTJP010000034 DASTJP010000000
VERSION     DASTJP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941914"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFQ23_00945"
     CDS             complement(<1..253)
                     /locus_tag="VFQ23_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905165.1"
                     /translation="MSERTKRIAILTGGPSAERGIALASAKFVAQHLDPLHYQSKTIL
                     IEPNGWIEQETGQAVDLNHFSLNEGLDRITFDFVFLIIHG"
     gene            complement(356..1105)
                     /locus_tag="VFQ23_00950"
     CDS             complement(356..1105)
                     /locus_tag="VFQ23_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET9905166.1"
                     /translation="MQRALIVEDEHLSREVLKSLLNTYCPEIHIVGEADSAKSAVIAI
                     QTTKPDVVFMDIELGDGTAFDVLRHIGKPQFQLIFTTAYDHYAVQAIKFNALDYLLKP
                     IDLEELKSAIAKITSSAYQPPSYEHFIANVTNTRNENPVITLSTSDSFEYIPVRDIIR
                     CEAQGAYTKFYIRNKSTLLVSKTLKEYEPLLQPYDFFRTHQSHLINLHDVERYLKGDG
                     GYIVLKDGMKIGVARTRRDEFLTAMKTLREV"
     gene            complement(1186..2028)
                     /locus_tag="VFQ23_00955"
     CDS             complement(1186..2028)
                     /locus_tag="VFQ23_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905167.1"
                     /translation="MSTLSLPEKSFLATLRTDRWWVEPLLVLCGLGAFILYATWAAFQ
                     GTHYWYDGGTAGFGGYLSPFYSPVVYTDPTMAGNAPLYHAIFGTWPSWWPSWAPASPA
                     LLILLFPLSFRFTCYYYRGAYYKAFSGTPPACAVGSVPRKRKGYYGETFLMVFQNLHR
                     YALYAAIIFIPILYADAIAAFFRGGQLGIGLGSVVLLINATLLACYTFGCHSWRHLIG
                     GRSDCFSCAGMGKLKFGVYQKSSWLNEKHKLFAWCSLIWVGVSDFYVRMVSSGAFTDP
                     NTWG"
BASE COUNT          633 a          506 c          565 g          568 t
ORIGIN      
        1 tgccgtgaat aatgagaaac acaaaatcga aggtgatcct gtcaagacct tcgttcaatg
       61 aaaagtgatt gagatctacg gcctgaccag tttcctgctc gatccagcca ttgggttcaa
      121 tcaggatggt cttgctttga taatgcaacg gatccaggtg ttgtgcaaca aattttgcac
      181 ttgccaatgc aataccgcgt tctgctgagg gtccgccagt gagaatggca atccgttttg
      241 tgcgttcaga catatgaaac aaatgtaata cttgtgacgt actggtgcgg atgaatttga
      301 gtaagcgtat gtactctctg aggatatgat acgtttggta acatacccga tcacgttata
      361 cttctctcag tgttttcatc gcggtaagga actcatcgcg acgtgttctg gctacgccaa
      421 ttttcatccc gtccttaagc acgatgtatc caccatcacc tttgaggtat cgttcgacat
      481 catggagatt gatcaggtgt gactggtgtg tcctgaaaaa gtcatagggc tggagcaagg
      541 gttcatattc ctttagtgtc ttactgacca ggagcgtgga cttattgcga atgtagaatt
      601 tggtatacgc accctgtgcc tcacaccgga tgatatcacg cacggggatg tattcgaaac
      661 tatcactcgt agatagcgtg atcacagggt tctcgttgcg tgtgttagtg acattcgcaa
      721 taaagtgttc atagcttgga ggttgatagg cgctgctcgt gatctttgcg atagcggact
      781 tgagttcttc caggtcaatc ggttttaaga gataatcgag ggcattgaat ttgatcgcct
      841 ggacagcgta gtggtcatac gcagttgtga aaatcaactg gaattgtggt ttgccaatgt
      901 gacgcaggac gtcgaatgca gtgccgtcac cgagttcaat atccataaac acaacatcgg
      961 gcttcgtcgt ttgaatggcg atcactgcag attttgcgct atccgcttcg ccaacgatgt
     1021 gaatttccgg acaataggta ttaaggaggc ttttgagaac ctcgcgactg agatgttcgt
     1081 cttcaacaat taacgccctt tgcatagatt aaaattaatt tgtgtggggc gaaacttgcg
     1141 atggggaaac cagaagcagg atatgactcg gcaactcaac ttacattacc cccaggtatt
     1201 cggatcggta aaggcaccgc tactcaccat cctgacatag aagtctgata cgccaaccca
     1261 tatcaggcta caccatgcga ataacttatg tttttcattc agccatgaag atttttgata
     1321 caccccgaat ttgagtttgc ccatcccggc acaggaaaaa caatcgctac gaccaccgat
     1381 caaatgccgc catgagtgac atccgaaagt atagcaggca agtaatgtgg cattgatgag
     1441 caggaccacg gatccgagac cgatacccaa ttgaccgcct ctgaagaatg cagcgatggc
     1501 atccgcatag agaattggga tgaagataat tgccgcatat aacgcatagc gatgcagatt
     1561 ttgaaagacc atcagaaatg tctcaccata atagcccttg cgttttcttg gaacagatcc
     1621 aacagcacat gcgggcggcg ttccagagaa ggctttatag tatgcgcccc gataataata
     1681 acaggtaaag cggaaagaca atggaaacaa gagaatgagg agtgcaggcg atgcaggtgc
     1741 ccatgatggc caccacgaag gccaggtccc gaagatggca tgatacaacg gtgcattccc
     1801 ggccattgtg gggtcggtat agactaccgg ggaataaaac ggtgaaagat atccgccaaa
     1861 acctgccgtg cctccatcat accagtaatg ggttccctgg aatgctgccc atgtagcgta
     1921 aagaataaac gcaccaagtc cgcatagcac cagcagaggc tcaacccacc agcgatctgt
     1981 ccggagcgtt gcgagaaacg atttttctgg aagtgaaaga gtactcatag gcatttacct
     2041 gtttcttcat tgcaaagtag ccaagttcat acaaacagga gtgagaaaat tgcccttagg
     2101 gtgattgtat accgtagcag tgaaaagatc gatatcatga gcgcaggtat tgatctggca
     2161 gcacgtccgg atgaactggt caaacgggcc ctctcagaat cctgcttggc gggtcacatt
     2221 ccgaaagcgt cataaagcgg ataactgcat tttggaggta aaatgtgaat at
//
LOCUS       DASTJP010000035         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_413289, whole genome shotgun sequence.
ACCESSION   DASTJP010000035 DASTJP010000000
VERSION     DASTJP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413289"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFQ23_00960"
     CDS             complement(<1..320)
                     /locus_tag="VFQ23_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905168.1"
                     /translation="MYLILLVIDDPDQLEDLLIAWEEAGVPSATVLFSTGMGRIRNLG
                     GWRDDMPLIPSLRDFYEAPENLNRTVFATANDEAQVDAIVTATNKVVGDLDKADTGLL
                     LVLP"
     gene            complement(320..1570)
                     /locus_tag="VFQ23_00965"
     CDS             complement(320..1570)
                     /locus_tag="VFQ23_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9905169.1"
                     /translation="MTTFLQLALLLSVILLSAKLAGYLSVRLGQPSVLGELLVGILLG
                     PSVIDILHFPFIDPALAHTVAELGELGVLLLMFIAGLELHLDELTRNTRVAGYGGFLG
                     VLVPTVLGWGAGRLFGLDNTVSIFLGLTLGATSVSISAQTLMELKVLRSRVGLSLLGA
                     AVFDDILVILSLSIFIAISAGTGSVGGVLWILVKMIAFLTLSVAFGWWVLPWVMRRIE
                     RLPISQGVLTLALVVMLAYGLAAELLGGMAAITGAFIAGLMMARSGERERVEQGVHAL
                     AYGLFVPIFFINIGLSVNARSLQIDMLVFALIITLVAIVGKWLGAGIGARVGGLSWRE
                     SIQLGAGMISRGEVGLIVAGVGINEGLVTSDEFSAIVVMVLVTTLVTPPILRSLFSQK
                     KTPVKPPKAEETTASVDTKNEEAN"
     gene            complement(1651..>2772)
                     /locus_tag="VFQ23_00970"
     CDS             complement(1651..>2772)
                     /locus_tag="VFQ23_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HET9905170.1"
                     /translation="IDFNTLGLLLGMMILVAMLEPTGFFQYVAVWAARASKGKPVRLF
                     ILLGSITTVLSMFLDNVTTVVLIAPVTLLICEILGVSPLPYLIAEGLLSNTGGVATLV
                     GDPPNVLIGSAAGLSFNDFLIYSLPIVAVAWVGALIFLRFLFREELSKQPENTEAVMS
                     LNPVESLDDPQTARRVLVVLGVAILLFFTHHILHISPSFIAVSAAAVALIWVQPDIQE
                     TFKRVEWSVLIFFGALFVMVGGLEAAGVLESIVGLLEGLARIPPVWFGVLLIWIVAGL
                     SAVVDNVPITIALIPVIHGLGETGMDITPLWWALAFGAGFGGNGTIIGSTANIIVASL
                     SEKTRTPITSRIWNRRGLPVMLATCAIASILFVLFYGLF"
BASE COUNT          798 a          777 c          648 g          549 t
ORIGIN      
        1 attggcagga caagcagcag tccagtatca gctttatcca aatcaccaac caccttattc
       61 gtcgcggtga caattgcatc tacctgtgct tcatcattgg ctgttgcaaa aacagtgcga
      121 ttcaaattct cgggtgcttc gtagaaatct ctcaaagatg gaatcaatgg catatcatcc
      181 cgccatccac ctaaattgcg aatacgtccc atgccagtgc tgaagaggac agttgcacta
      241 ggcacgcccg cctcttccca ggcgatgagc aaatcctcca gctgatctgg gtcatcgata
      301 acaagcaaga tcaggtacat cagtttgcct cctcattttt tgtgtcaacc gaagccgtgg
      361 tttcttcggc tttgggcggt tttacaggtg tctttttctg cgaaaacaaa ctccttagga
      421 tcggcggtgt caccagcgta gtaaccaata ccattaccac aatggctgaa aattcatccg
      481 aggttactag tccttcatta ataccaacac ctgccacgat caagccaacc tcgccgcgcg
      541 agatcatgcc tgcccctaac tggatcgatt cgcgccagct caatccaccc acccttgccc
      601 cgatccccgc gcccagccac tttccaacga tagcaacaag agtaatgatc agtgcaaata
      661 ccagcatatc aatctgtagt gaacgcgcat tgacggaaag cccaatgttg atgaaaaaga
      721 ttggtacgaa gagaccgtat gccagggcat gtacaccctg ttccacgcgt tccctttctc
      781 cactccgcgc catcatgaga ccagcgataa aggcgcccgt gatcgcagcc atccctccta
      841 agagctctgc agccagtcca taggcgagca tcacgaccag tgccagagtt agcacaccct
      901 ggctaatggg caggcgttca attcggcgca tcacccatgg caatacccac catccaaacg
      961 cgacggagag agtcaggaaa gcaatcatct tcaccaggat ccaaagcacg ccacccacac
     1021 tgccggtgcc tgcggatatg gcgatgaaga tcgatagcga caaaataacc aggatatcgt
     1081 caaagacagc agcgcccagg aggctcagtc caacacgact acgcagtacc ttgagttcca
     1141 tcagggtctg cgcggagatg cttacgctgg tcgcacccag ggttagacca agaaatattg
     1201 aaaccgtgtt atccaggcca aaaagccttc ccgcacccca tcccagtaca gtaggaacaa
     1261 gaacgccgag gaaacctcca tagcctgcca cacgtgtgtt gcgagtaagt tcatccaggt
     1321 gcagctccag gcccgcgatg aacatcaaga gcaggactcc caattcccca agctccgcaa
     1381 cggtatgagc tagcgcggga tctatgaaag ggaagtggag aatatcgatg acagaaggac
     1441 ctaacagaat accaaccaac aactctccta acacggatgg ctgtcccagc cgcacactga
     1501 gatagcctgc cagctttgca gatagcagaa ttacagaaag taacagggcg agctgcagaa
     1561 aggtggtcat cgtactaaac ccatgcgcca attaatgttc agtataaagg ttccaaacat
     1621 gctgattaaa accattactt cttgcctcct tcaaaacagt ccatagaaga gcacaaatag
     1681 gatacttgcg atcgcgcaag tagccagcat cactggaaga ccgcggcgat tccatatgcg
     1741 cgaggtaatt ggcgtacgtg ttttttcgga gaggcttgcg acaatgatat tggccgttga
     1801 gccaataatc gttccgtttc cgccaaaacc cgcaccgaat gccaatgccc accagagtgg
     1861 tgtgatatcc attcctgttt cacccagacc atgaatgaca gggatcaagg caatcgtgat
     1921 cgggacatta tcgacaactg cagaaagacc agcgacgatc cagatcagaa gtaccccaaa
     1981 ccagacaggc ggaatacgcg ccaggccctc taagaggccg acaatggact ccagtacccc
     2041 tgccgcttcc aaaccgccaa ccatcacaaa gagtgcgcca aagaaaatga gaacactcca
     2101 ttccactcgt ttgaaggttt cctggatatc aggttgcacc cagatcagcg cgacggccgc
     2161 tgcgctgacg gcgataaagg atgggctgat atgcaagata tgatgggtga agaacagcag
     2221 gatggcgaca cccaagacca ccaagacgcg acgcgccgtt tgcgggtcat cgagagactc
     2281 cacaggattc aaactcatca ctgcctcagt gttttctggt tgcttcgata actcctcgcg
     2341 gaacagaaaa cgcagaaata tcaatgcgcc gacccaggcg actgccacaa tgggcaggga
     2401 atagattaga aagtcattga aagatagtcc agcagcagag ccaatcaaca cgtttggcgg
     2461 atcgcctaca agagttgcta caccgccggt attggaaagt aaaccctcgg cgatcaaata
     2521 gggaagtgga cttaccccga gaatttcaca gatcagaagt gttaccggtg cgatcaagac
     2581 aacggttgtt acgttgtcca gaaacatcga aagtacagtc gtgatgctgc ccagtaaaat
     2641 gaataaacgc acaggcttgc cctttgatgc acgagccgcc cagaccgcca catactggaa
     2701 aaatccagtg ggttcgagca tggcaaccag gatcatcatg cccagcaaaa gaccaagcgt
     2761 attgaagtca at
//
LOCUS       DASTJP010000036         7232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1470578, whole genome shotgun sequence.
ACCESSION   DASTJP010000036 DASTJP010000000
VERSION     DASTJP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470578"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFQ23_00975"
     CDS             complement(<1..823)
                     /locus_tag="VFQ23_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905171.1"
                     /translation="MQRILFGNNWWHRFLPLAFMLAGAGITVIGIAADLLDFGGPRGI
                     GPRQVSLALSGFAVFLAGVDLISTGRQRYIIEWLMVALATITVVFAADLLVINGLPDI
                     AAKHLAVASLGFSILSMGTIPVLLGDQRNISAGLNLFSLEKLEVGKFLSIALQLGLLV
                     LVIRLFRLENQAIYHNLMLLTFYGFLIHYLLPSHHRLPFFLFISLAAVAGILGFINSI
                     RLIGIGLGLIGICHLPISYNKRVAVLFLAGAAMAAVRTGWIQLSGLDVIWPILASM"
     gene            complement(839..1099)
                     /locus_tag="VFQ23_00980"
     CDS             complement(839..1099)
                     /locus_tag="VFQ23_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9905172.1"
                     /translation="MTNLNVQEVKEIVKEYILKEFLPGESPEALTDSTPLITGGILDS
                     LATIKLVVFLEERFKIQIEAHETMADYLDTVADIAQLVSSKL"
     gene            complement(1158..2225)
                     /locus_tag="VFQ23_00985"
     CDS             complement(1158..2225)
                     /locus_tag="VFQ23_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9905173.1"
                     /translation="MKTNSRSILINLSLIFASILFALFLSEIALRLIGFKPLYVSPER
                     DRFWKYDSLLGWVHQPGQEGIFETPQFRTVVRINETGLRDRQRLYKRQGNGERIMVLG
                     DSFAWGYGVEESERFSQLLEKSLEVEVINAGVSGYSTDQELLWYKNEGIKYDTDLVIL
                     VIAGNDVGDNEQQLVSTIYYKPRFVLENDQLVLTGSPVPETTQQGKIIYSLSQRSSLA
                     FFLVQRYFDLRSLFGKTKSNSDHVTLPISGDTVAKEPFKLTIAIIDEIKNIAESRKAK
                     FMIVTTERWWNYASEETYKDFLTALRRKGFLVLDVESMPGFDPEEMIIPNDGHWNQSG
                     HNFVSQKIKSFIETHQLLRLH"
     gene            complement(2304..4988)
                     /locus_tag="VFQ23_00990"
     CDS             complement(2304..4988)
                     /locus_tag="VFQ23_00990"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET9905174.1"
                     /translation="MKKTPTLRRFGWLYRKLSLLFACAAFITIMAPSWVIAQSTDDSW
                     TDPLNLSHSGIAQNPSIVTDTDGVVHVVWRDDFGKYIYTRFEENQWSAPKTTDWDIVF
                     NWSPPLTSDDQIAIYTGPNPLFVSGPGEYVFAFWVSPQGGLFTSKLENQKIGETSWEY
                     ARLIASGVGSFAVSFDARGNWHLVYLSRAYDPVRPVGIYYTRSKNEGQSWIPPRLLQE
                     SHYFRALAEGESNLSVATAGPEEAQSVYVAWGNKLRRQVFLAQSADGGETWEQPRLIA
                     GPAPDPSLGAPFNIQVGANQESVILLWQSGQPQGTCSQIFQYSKDAGVTWSDPELIIN
                     EDLIGCAQSNQFVPVLASNTEGPLYLLTKGASQVFVSAWNGSQWSQPQEQSILSGFEE
                     PEIFSEVIFGCQQASLFGERLYIVGCDEGGGGDVWVTSRTIDANLSGSTAPIWSQLSP
                     VTEDSLELESIELLATDDGLIHAFFSKHQDSAIYYTYWNGELWSRISPVMVLTDEAAG
                     SPEISAGVGNELFLIARSNRGTLYYSRAISGNAASQSRWSPPTQLEIKHDGQVGSADI
                     AWDVAGTVYIAYSVPVNSERGIYLIESQNQGGAWSAPLQLFDGAAAGFDFIGAPSLLT
                     SEEGVLDITWSVQSIQGDGAPQPLSLFYTHSMDSGQTFNEAQLIVEEPVSWREFGIDD
                     DNHLHLLWQPQDSPLTVWDQISMDGGNTWQFPQGLQGEGSLVATTSDAAGRLHVMGVG
                     EEEVGHWLWDDGRWRSEAPLHLPLSAQQKRTVEIFSAAVNLHGQMVVVLAESTGEGDG
                     REMNLLYSTRMLALPLEQTTTEVVATQTVVTPSITAATSTPEPLLTPTFTLDNQGQTD
                     GVETNDPVSPFIMALLPVALLLIVVLGVVIRRIAQAKN"
     gene            complement(5017..6645)
                     /locus_tag="VFQ23_00995"
     CDS             complement(5017..6645)
                     /locus_tag="VFQ23_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905175.1"
                     /translation="MAVFGWNGELNTDEIFESFAKMRKFYYYAHLPILLITIISSIFF
                     FSFTAEDAYITYRYAENFVNTGSLVFNQGEPINAMTSPFHALLSSGLFYATGNIVLSN
                     KLLAFLLLIISTILIWRRFSNYPQWQVLVLILVLMPPSVLLWTFGGLETPFLLFLTTA
                     TALLVERTHPFNLKFLFLTFFLAGLAFLTRYDSILFFLPLTLYVVSKARSIIHVVIAA
                     IAASVLPLAWFLVSIYYYGDLLPTSFYVKTPTGSLGGLIFNGAYIGSYLHYVGIIPVV
                     ALVFVLLVPKHRTIDIIYEQFKGMWWLYIGLFLEIMYGLTMATHHMMFSFRFFVPYIP
                     PAAILTVDLVRRALEVRNLDLSKGRPAYMLMGFLLCLVLFQLYQITYTYKYSVNGISM
                     LGEYRSLGIHDYVGFMEILKKESLDIKNHWNMIVGESSHTPRILTYAAGMLPYTYQES
                     YIYEKLVSYRHCHDRYRQGLYADYIHIIAPRQGPVNQQLPKPEQSYELVSSYEMFFDG
                     SMQKFLVYYNPQPEPHNLTNRIYEPCELGINSEN"
     gene            complement(6579..>7232)
                     /locus_tag="VFQ23_01000"
     CDS             complement(6579..>7232)
                     /locus_tag="VFQ23_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9905176.1"
                     /translation="GALVLVTLYVLVGLKFNDKSGWLSAFVSIAAIITARSWRAGLAL
                     TPVAIIAALYMWSGLVSTDEYSISTRFDAWIIMGQIIKLNPILGLGFANYYWYTPLFP
                     IRGYAVSFNSHNNYVDIVAQTGLVGLFCVLWFFWEVGWLGWRLRKDAPSGFAQAYVYG
                     ALGVLAGMVVAGMFGDWVLPFFYNIGLNGFRSSMLGWLFLGGMVSLTQMKYSKVSPK"
BASE COUNT         2041 a         1745 c         1636 g         1810 t
ORIGIN      
        1 acatcgaggc caaaatcggc cagatcacat caagcccgga gagctggatc caacctgtcc
       61 gtactgcggc cattgccgcg ccggccaaga acagtaccgc gacccgcttg ttatacgaga
      121 taggaaggtg acagattccg ataaggccaa gaccaattcc gattaaacga atactgttga
      181 tgaagccaag gattccggcg acggcagcta aagagataaa aaggaaaaag ggtaggcgat
      241 ggtgggaagg aagcaaatag tgaatcagaa aaccataaaa cgtcagcagc ataaggttat
      301 ggtaaatggc ttggttttcc aatcgaaaca agcgaattac taaaaccaaa aggccgagtt
      361 gaagagcaat acttaggaat ttgccgactt ccagcttctc cagggagaaa agattcagcc
      421 ccgcactgat atttcgctga tcacccaata agaccgggat ggtgcccata gaaagaatgc
      481 taaatccaag agaggctact gctaggtgtt tggcggcaat atcaggtaag ccatttataa
      541 caagtaggtc agcagcgaag acaacggtaa ttgttgctaa tgcaaccata agccattcga
      601 tgatgtagcg ctgtctgcca gttgaaatta agtccactcc agccaggaat acagcgaagc
      661 cactcaatgc cagggagact tgcctggggc caataccgcg cggcccgcca aaatctagaa
      721 gatcagcagc tatacctata acggtaatgc ctgcaccagc cagcatgaaa gccagaggca
      781 gaaacctgtg ccaccaattg tttccaaaaa gaatacgttg cacagccaaa tctctcctct
      841 acagttttga actgactagt tgcgctatat cagctactgt atccaaatag tcggccattg
      901 tctcatgagc ttcaatttga atcttaaacc gttcctccag gaataccacc agctttatcg
      961 tggcaagtga gtcgaggatt ccgccagtga tgagtggagt cgagtcagtc agcgcctccg
     1021 ggctttctcc ggggaggaac tctttgagga tatattcttt tacgatttct tttacttctt
     1081 gcacatttaa gtttgtcata attatccctt ttcataataa atctggaaga taggctgatg
     1141 tgcccgctga tgaccgatca atgcagtcgg aggagctggt gggtttcaat aaacgactta
     1201 atcttttgcg acacaaaatt atgacccgat tgattccaat gcccatcatt ggggataatc
     1261 atttcttccg ggtcaaatcc tggcattgat tcaacatcca ataccagaaa tccttttcgt
     1321 cgtaatgccg ttaggaagtc tttataagtt tcttccgatg cataattcca ccacctctca
     1381 gtcgtcacga tcatgaactt cgctttcctt gattcggcga tattcttaat ttcatcgatg
     1441 atggcgatag tgagcttaaa gggttctttt gcaacagtgt cgccagagat tggcaatgtg
     1501 acgtgatctg aattactctt cgttttcccg aacagggacc gtaggtcaaa atatctttgg
     1561 accagaaaaa aagctagaga agagcgctgc gaaagcgaat aaataatttt tccctgttga
     1621 gtggtctcgg gcactggcga accggtaagc acaagctgat cgttttcaag cacaaatctt
     1681 ggcttatagt aaatggtgct gacgagttgt tgctcattat cccccacatc gtttccggca
     1741 atcactaata tgacaaggtc ggtatcgtat ttaatgcctt cgtttttata ccagagcaat
     1801 tcttggtcag tactgtatcc cgagactcca gcattaatca cctcaacttc cagtgatttt
     1861 tcgagcaatt gtgagaatct ctctgattcc tccacaccat aaccccatgc aaacgagtcg
     1921 cccagtacca ttatgcgctc gccattacct tgacgcttat acaggcgctg gcggtctcgt
     1981 aaaccggttt cgttgattcg cacaactgta cgaaactgcg gggtttcaaa tatgccttct
     2041 tgtcctggct ggtggaccca gcccagtaga gaatcatatt tccaaaaacg atctctctcc
     2101 gggctaacat acaatggctt gaaacctatg agtcgcagcg ctatttcact cagaaataag
     2161 gcaaagagga tggaagcgaa gatcaaagat agattaataa gtatgctgcg gctattagtt
     2221 ttcatcagtt aggtaccatt ttatttatct ggcttactgc ggcaggtccc tgggagccaa
     2281 ttacggcaaa tttgcctatc ttatcaattc tttgcttgcg caatccgtcg aatcacaact
     2341 ccaaggacaa ctataagaag cagcgcgacc ggaagcaaag ccataatgaa tggagaaacg
     2401 ggatcgttgg tttctacacc atcggtttgt ccctgattat caagcgtaaa ggtcggggtc
     2461 aacaagggtt cgggggtcga cgttgctgcg gttatcgagg gagttaccac tgtctgtgtc
     2521 gcgacgactt cggttgttgt ctgttctaaa ggtaaagcca gcatacgagt ggaatatagt
     2581 aggttcattt ccctcccgtc gccttcaccc gttgattctg ccagaaccac caccatttgc
     2641 ccatgcaggt tgaccgcagc tgaaaatatt tccacagttc ttttctgttg agcggacaaa
     2701 ggtaaatgta aaggcgcttc ggatctccac cgaccatcat cccaaagcca gtgacccact
     2761 tcctcttcgc ctacacccat tacgtgcagt cttccagcag catcactcgt agtcgccaca
     2821 agacttccct cgccctgaag tccttgtgga aactgccatg tattcccacc atccatcgag
     2881 atctgatccc aaacggttag aggcgagtcc tgcggttgcc agagtagatg taaatgatta
     2941 tcgtcatcaa tcccaaattc tcgccagctc acaggttcct caacaatgag ctgggcttcg
     3001 ttgaaggtct gcccgctatc catggagtgc gtgtaaaaga gcgacaatgg ttgtggagct
     3061 ccgtctccct gaatcgattg tacactccag gtaatatcca atacgccttc ttcagacgtc
     3121 agcaacgaag gtgccccaat gaaatcaaaa cctgctgctg ctccatcaaa caattgcaac
     3181 ggcgctgacc aggctccacc ttgattctgt gactcaatca gatatatccc gcgctcactg
     3241 ttcacaggaa ccgaataggc tatgtacacc gttcctgcga cgtcccaggc gatatctgcc
     3301 gaaccaactt gaccatcatg cttgatttca agctgtgttg gcggagacca gcgggattgt
     3361 gaagcagcat tgccgctgat cgcccggcta tagtaaagtg ttccacgatt gcttcgggcg
     3421 atgaggaaca actcgtttcc cactccagcg gatatctcgg gcgagcctgc tgcctcatct
     3481 gtcagaacca ttactggaga gatacgggac cacaactcgc cattccagta tgtgtaatag
     3541 atggccgaat cctgatgctt gctgaaaaag gcgtggatta gaccatcatc tgtagccagc
     3601 agctcaatcg actctagttc aaggctatcc tcggtaactg gcgaaagttg gctccagatc
     3661 ggtgcagtag acccggacag atttgcgtcg atggttcgcg aagtgaccca aacgtcgcca
     3721 cctccgcctt cgtcgcagcc aacgatgtaa agccgctctc caaacaagga ggcctgctgg
     3781 caaccaaaga ttacctcaga aaaaatctcg ggctcttcaa atcccgataa aatcgattgt
     3841 tcctgtggct gactccattg acttccgttc caggctgaca cgaacacttg gcttgcacct
     3901 ttagtcagca agtataaagg gccttctgta ttactcgcca agaccggcac aaattggttt
     3961 gattgtgcgc agccaatcaa atcctcattg attattagtt cgggatcgct ccaggtgacg
     4021 cctgcatctt tagagtattg aaagatctga ctgcatgtac cttggggctg accgctttgc
     4081 cagagcagga tgacgctttc ctgattcgct ccgacttgaa tattgaatgg cgcacccaaa
     4141 ctcggatcgg gcgctggacc tgcaatgagc cggggttgtt cccaggtttc tcccccgtcc
     4201 gccgattgag ctaggaagac ctgccgacgc agcttgtttc cccaggcaac atatacgctt
     4261 tgcgcttcct ctggaccggc tgtggcaaca ctaaggttgc tctctccctc ggccagcgcc
     4321 cggaagtaat gggattcctg caataatctt ggtggaatcc aactctgacc ctcatttttg
     4381 gagcgagtgt aataaatgcc aacaggccgc acaggatcgt aggccctgct caagtaaacc
     4441 agatgccaat taccgcgggc gtcaaaagat acagcaaaag aacccactcc agaagctatg
     4501 agccgagcat attcccagga tgtttctccg attttttggt tttctagctt gctcgtgaat
     4561 aatccacctt gcggcgagac ccagaaggca aatacatatt cgcccggacc agacacgaac
     4621 aatggatttg gacctgtgta tatagcgatt tgatcgtcgg aagtgagtgg tggactccaa
     4681 ttgaacacga tatcccagtc tgttgtcttt ggtgcgctcc attgattttc ttcaaaacgc
     4741 gtatatatgt atttcccgaa atcatctcgc caaaccacat gcacaactcc gtctgtatcg
     4801 gtcacaattg acggattctg cgcaataccg gagtgggaaa ggttcaaagg atctgtccaa
     4861 gagtcatcgg tagattgggc tatgacccat gaaggtgcca tgattgtgat gaacgcggcg
     4921 caagcaaaca ataagctcaa cttccggtac aaccatccaa atcgtcgcaa tgttggtgtt
     4981 tttttcattt tgatagatta gatttcacgg ctttcgttag ttttcggagt ttatacccaa
     5041 ttcacagggt tcgtagatcc ggttagtgag gttatgaggt tcaggttgcg gattataata
     5101 aacgaggaac ttttgcatcg atccatcaaa aaacatctcg taagaggata cgagttcgta
     5161 gctttgttca ggcttcggta gctgctgatt taccgggccc tgcctggggg caatgatatg
     5221 aatataatcg gcatacagcc cttgacgata tcgatcgtgg cagtggcgat aagaaactaa
     5281 tttctcatag atatatgatt cttgatacgt ataaggcaac attccggctg cgtaggttag
     5341 gatgcgtgga gtgtggctac tttctccaac tatcatattc caatgatttt tgatgtctaa
     5401 ggactccttt ttcagaatct ccatgaaccc tacataatcg tggataccaa gtgatctata
     5461 ctcccccaac attgaaatcc cgtttacaga atacttgtaa gtgtatgtaa tttggtatag
     5521 ctggaacagt accaggcaca aaagaaatcc cattaacatg tatgcaggtc ttcctttgga
     5581 caggtctagg ttcctgactt caagtgcacg gcgaacaaga tccacggtaa gaatggcagc
     5641 tggaggtatg tacggaacaa agaagcgaaa agaaaacatc atatggtgcg tggccattgt
     5701 caacccatac ataatctcta agaatagtcc aatataaagc caccacatgc ctttgaactg
     5761 ttcgtatatg atatcaatgg tcctatgttt tggaaccaac agaacaaaaa ctagagctac
     5821 tacaggaatt attccgacat agtgcaggta ggaacctata taagctccat taaaaatcaa
     5881 tcctcccaag ctcccggttg gtgtcttaac gtagaatgag gtcgggagca aatcaccata
     5941 gtaatagatg ctgacgagga accaagccag gggcaatact gaggctgcta ttgctgcaat
     6001 aactacatgt atgattgatc gtgctttcga taccacatat agcgttaaag gtaggaagaa
     6061 tagtattgag tcataacgtg taagaaaagc cagcccagct aaaaaaaatg tcaagaatag
     6121 aaacttaaga ttgaagggat gcgtacgctc cactaacaaa gctgtcgcag ttgtaagaaa
     6181 aagaagaaag ggggtttcca gtccaccaaa agtccacaac aagactgacg gcggcatcag
     6241 gactaatatt agaactaaaa cttgccattg cgggtaattg ctaaatcgac gccatattaa
     6301 tatcgttgat atgatcaaaa gaagaaaagc cagaagcttg ttactaagaa caatattgcc
     6361 tgtcgcatag aataatcctg acgagagcag ggcgtggaaa ggtgaagtca tcgcattgat
     6421 gggttcgccc tggttaaaga ccagtgagcc tgtatttaca aaattctctg cataacgata
     6481 cgttatgtac gcatcttcag cagtgaagct gaaaaagaat attgagctaa tgatagttat
     6541 tagcaatatg ggaagatggg catagtaata aaattttctc attttggcga aactttcgaa
     6601 tatttcatct gtgttaagct caccattcca cccaaaaaca gccaacccaa catgctggat
     6661 cggaatccgt tcaaaccaat attatagaaa aaaggcagaa cccaatcccc aaacattccc
     6721 gccacaacca taccggctag tacgcccaac gcaccgtaaa cataagcctg ggcaaatcca
     6781 cttggtgcat ctttgcggag cctccagccc aaccagccga cttcccagaa gaaccacagg
     6841 acgcagaaaa gtcccactaa tcctgtctgg gccacaatat ctacatagtt attgtgagaa
     6901 ttgaaactga ctgcataacc cctaattgga aatagaggtg tataccagta atagttggca
     6961 aagcccaacc ctaggatagg attgagcttg attatctgac ccatgatgat ccacgcatca
     7021 aaacgcgtgg agatactgta ttcatcagtc gaaacaagcc cggaccacat atacaaagca
     7081 gctatgattg cgactggtgt cagggcaagg cctgcacgcc acgaacgagc ggtgatgatg
     7141 gccgcgatgc tgacaaatgc cgatagccag cctgatttat cgttaaattt caatcccacc
     7201 aaaacgtaaa gggtgacgag tactagcgca cc
//
LOCUS       DASTJP010000037         6730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1575730, whole genome shotgun sequence.
ACCESSION   DASTJP010000037 DASTJP010000000
VERSION     DASTJP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575730"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFQ23_01005"
     CDS             <1..886
                     /locus_tag="VFQ23_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905177.1"
                     /translation="ILACLRQWGIQPTFEHRAPLSFEVYDSKGDCHEVTLPQPFFYLL
                     KRGVERGSLEQALKAQALERGVNIRYNQPASKADVEIWAAGARSAGFFLGTGITFRTS
                     HPDIVAGLVDTLAAPKAYGYLVIVDGFATLSIVLTKDFKNAREYLAESIKIFRRHKTL
                     DMEDVRQTSGFGGLMDAFWQPVTQPLVVGEAAGLQDFLWGFGIRHAIHSGHLAARAVH
                     ENLNYPQLISREIHPLVRTSIINRAMYNRVGNWGYRTLIRHFAPSQNLSNQIRPWYRG
                     LPLHRILWPIVERRYRPK"
     gene            905..1465
                     /locus_tag="VFQ23_01010"
     CDS             905..1465
                     /locus_tag="VFQ23_01010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905178.1"
                     /translation="MSSRFDITLRDENLKLEVLHADQLAPERLAEIHALCNRAYDADL
                     GPLFATFSDTTHVLGHWGPAIVSHAMWVTRWLQPGNQLQLRTAYVEMVATEPPFQGRG
                     FATAVMRQLALAIQDFEMGGLSPAEPVLYEKLGWVFWKGPHFIRTSDGLLSAPEDQIM
                     ILKLPKTPPLDLTLSLSAEWREGELW"
     gene            complement(1468..1674)
                     /locus_tag="VFQ23_01015"
     CDS             complement(1468..1674)
                     /locus_tag="VFQ23_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9905179.1"
                     /translation="MPIITNIDVMLAKRKMSVTELSEKVGITMANLSILKNGKAKAIR
                     LSTLDAICKALGCQPGDILEYKSD"
     gene            complement(1684..2166)
                     /locus_tag="VFQ23_01020"
     CDS             complement(1684..2166)
                     /locus_tag="VFQ23_01020"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HET9905180.1"
                     /translation="MRLKQGSTLFLKIVISLIGIVTLAAMIRLPQLEGRSANLDLISI
                     YEDPLIIYTFLASIPFFIALYQGFKLLGYVDQNRVFSQLALEAVKKIKYCAMSLPVLI
                     IIGEAYIIFGANGDDAAGPVALGIFATFASIVIATGVAIFERLLQNAVDLKSENDFTV
                     "
     gene            2518..3090
                     /locus_tag="VFQ23_01025"
     CDS             2518..3090
                     /locus_tag="VFQ23_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905181.1"
                     /translation="MSRNLPPHPNLEYLKKQAKDLLHELKQRNPDAQLADAQFALARE
                     YGFASWPNLKTYVDSQSNVEPAELSPFAGKWVANLLKSNRHPANPFQSATIEFIVDNN
                     TVTIIDVVIDESGQEVTGKNTLRVDGVEYATENGSGYVLTAKWKGPHIIETEARKEGQ
                     VAGWGRYEVSDDGQTLTISGEEQKIILNRP"
     gene            3642..4517
                     /locus_tag="VFQ23_01030"
     CDS             3642..4517
                     /locus_tag="VFQ23_01030"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9905182.1"
                     /translation="MSATEVARGIKGKTSAGNSIEPFRPEWIDYTPHPKVGTDSQPLL
                     RRINGQLIEPHYGVFGADNRQVYYPSGYPWTCIGKILVWNDFSQPFPAWSASGVLIGG
                     RVVLTAGHVAPWGSNNWAMQFIPSFYNGGSTLGAGVSSWVSDYRGYNTGNHVSAWDML
                     VLRLYTPLGNTYGYFGTKDYHSSWQGGNYWTLAGYPGAIAGGNRPSRQMWWPVLDDDA
                     DGSAEEVEYEADSSGGNSGGPVFGFWNGQPYAIATHSGGSKTTFLWWTLEDTNVGAGG
                     GALNSLVAWARSNWP"
     gene            complement(4588..5595)
                     /locus_tag="VFQ23_01035"
     CDS             complement(4588..5595)
                     /locus_tag="VFQ23_01035"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9905183.1"
                     /translation="MALFSKDLGIDLGTMFTRLADSSRVLLEEPTIVAIEVADQKMVA
                     VGQEARDMYGRVPESIEVARPLKNGVIADYEITETLLSYLLQRVSGSMRIFRPRVMIS
                     VPYGVTSVERRAVYEAVLEAGSREAYLIQQPLAAALGVDLPINSPSGNMVICLGGGCT
                     EAAVLAMYGIVSAETLRAGGIDLDDAIVNYVRRKYGIVVGQVTAEQLKMKIGAAVPQD
                     TENSMEVQGQDQVTGLPRPVTLTTGEIVEALQEPLKAVIETGRRVLEKTPPELVADII
                     DRGVALCGSGALLRGVDKLLTKSLGIPAYLVDNPATCVVEGAVKSLPMYNILRRSLPQ
                     V"
     gene            6085..>6730
                     /locus_tag="VFQ23_01040"
     CDS             6085..>6730
                     /locus_tag="VFQ23_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905184.1"
                     /translation="MLLRGDENPLSRRMKRYWKVAVLANIKDDTQPKPDGVPPDAFAD
                     FDHIETIDFIRAALETDGHQTVFIHADADLPYALRDEKPDICFNIAEGLGGDAREAQV
                     PALLEMMRIPYTGSRVLTNGISLDKTLTKRIWRDRRLPVAPFQEFAAGDEPLRPELNF
                     PLFVKPAREGTGMGVDTKAIVKNEKELRERAMYIIDTYQQPALVETFLPGREFTV"
BASE COUNT         1750 a         1671 c         1651 g         1658 t
ORIGIN      
        1 catattggct tgtcttcgcc agtgggggat tcaaccgacg tttgaacacc gcgcgccgct
       61 ctcctttgaa gtgtacgact cgaaagggga ctgtcatgaa gttacgctgc cacaaccttt
      121 tttctacttg ctcaaacgcg gtgtggaacg cggctccctt gaacaagcac taaaagcgca
      181 ggcgctggag aggggtgtca atattcgcta caaccagcca gcttcaaaag cggatgtgga
      241 gatctgggca gcgggcgcgc gctcggcagg gtttttcctg ggcacaggaa ttacctttcg
      301 cacctcccat ccggatatcg ttgccggatt ggtggataca cttgctgcgc ccaaagccta
      361 tggctatttg gtgatcgtgg atggttttgc caccttatcc attgtgctta cgaaagattt
      421 caaaaatgcc agggaatatc tggcagagtc gataaaaata ttccgtcgcc acaaaactct
      481 ggacatggaa gatgtccggc agacttcggg ctttgggggc ttgatggatg cattttggca
      541 gcctgtcact cagcccctgg ttgtcggcga agcggcaggg ctccaggatt tcttatgggg
      601 attcggaatc cgccatgcaa ttcattcggg acatttggcg gcgcgcgccg tccatgaaaa
      661 cctgaactac cctcaactta tctcccgtga aatccatccg ttggtgcgca cttcgattat
      721 caatcgtgct atgtataacc gagtagggaa ttgggggtac agaacgttga tccgacactt
      781 tgcgccgtct cagaatcttt ccaaccagat ccgtccatgg tatcgcggct tgcccctgca
      841 tcgaatcctg tggccgatcg tagagcgtcg ctatcgtccg aaatgaaatc gcctcaccta
      901 ctcaatgagt agtcgttttg atataacgct gagggatgaa aatctgaaat tagaagtcct
      961 tcatgcggat caacttgctc cggaacggct tgcagagatt cacgcccttt gtaatcgtgc
     1021 gtatgacgca gacctgggac ccttattcgc gacctttagc gatacaactc acgttctcgg
     1081 ccactgggga ccagcgatag tcagccatgc gatgtgggtg acccgttggc ttcagccggg
     1141 caaccaactt caattgcgaa ctgcctatgt tgagatggta gctactgagc ctccgttcca
     1201 ggggcgtggc tttgccacgg cggtaatgcg tcagctggca cttgccattc aggattttga
     1261 aatgggaggt ttgtctcccg cagaaccggt actatacgag aaattgggtt gggtcttttg
     1321 gaaaggaccg cacttcatcc ggaccagtga tggtttgctt tccgcccctg aagaccagat
     1381 catgatctta aagctgccta aaaccccacc ccttgatcta actttatcct tatctgccga
     1441 gtggcgtgaa ggtgagttgt ggtaaagcta gtcgcttttg tattccagaa tatccccagg
     1501 ctgacaacct aacgctttac agatcgcgtc caaagttgag aggcgaatcg ccttggcttt
     1561 tccatttttt agaatggaaa gattcgccat cgtgattccc actttttctg atagttccgt
     1621 gacgctcatt ttgcgcttgg ctagcatcac atcgatattg gttatgattg gcataattgc
     1681 atctcaaacc gtaaagtcgt tttcagattt tagatctacc gcattttgga gaagtcgttc
     1741 aaaaatcgcc acaccagttg caatgacgat cgaggcaaat gtagcgaaaa tacctaaggc
     1801 aacgggacct gcagcatcat caccgttggc accgaaaata atataagctt ctccaatgat
     1861 gattaagaca gggagactca tcgcgcagta ttttatcttt ttcactgctt cgagtgctaa
     1921 ttgagaaaaa accctatttt gatcaacata acctaacagc ttaaatccct gatataacgc
     1981 aataaaaaat ggaattgaag ctagaaaggt atagataata agcggatctt catagatact
     2041 gatcagatct aaattagcag atcttccctc cagttgagga agcctaatca tggcggccag
     2101 cgtcacaatg ccgatcagag aaatcacaat ctttaggaat agagtagaac cttgttttag
     2161 cctcataact acctcgcttc gtaattgata gttacattgt acacaagatt tatcgttttt
     2221 cgatatatta ttattgttat tcgttattgt attgagactg gggaaagtct tgttctcgcc
     2281 gtccacgggt agtaatggtg tcctggcttg cggcaattaa cgcgcttgcc gcccaacaaa
     2341 gcactctttc aaacatcttg ctcctcccct tgtcccgcat tagaataagg ttgctgcagt
     2401 tccaggctgc gccccgtccg cgggaaggga tgatcccacc tgagcttttc aattcaatcg
     2461 agtattgaca atctctcttt tgcccgtcag cggacaccgg gcgacaaagg agtagtcatg
     2521 tctcgtaatc taccacccca ccccaatctc gaatatctta agaaacaggc aaaagatttg
     2581 ctgcacgaat tgaagcagcg aaatcctgac gcacagcttg ccgatgcaca atttgcactg
     2641 gcacgcgaat atggttttgc cagttggccc aatctcaaga cctatgttga ctcacaatca
     2701 aacgtagagc cagcggagtt aagtccattt gcaggaaagt gggtcgcaaa tctgctgaaa
     2761 tctaaccgac atccagccaa tcccttccaa agcgcaacga tagagtttat tgtggataac
     2821 aacacagtga cgatcattga cgttgtcatc gacgaatcag gtcaggaagt gaccggcaaa
     2881 aacacactcc gtgttgatgg tgtggagtac gccacggaaa atggcagtgg atatgtgttg
     2941 actgcgaagt ggaagggtcc gcatatcatt gaaacagaag caagaaagga gggtcaggtg
     3001 gctggctggg gaagatatga agtttcggat gatggtcaaa cgttgaccat ttctggtgag
     3061 gagcaaaaga ttatcctgaa tcgcccttag gaattcaaag gcaaatcggc aagattattg
     3121 caaatagaat caacaaaaac ggtcacgctc tgcgcgaccg ttttgctatt acgcctatac
     3181 aatggtgtct caaattattc acgttgaact cgattcagat cagcctcatc gaaaatagag
     3241 ggaatccttc atgacagtac gtcgggccgt cgctacaatt tgatagatca tatatataaa
     3301 caagccatat tatagagatc gtacagtttt tccaaacatt agtttcagca aaggagaata
     3361 aaatgccaac taagaaacca agcagtggaa aacgtgtaag gcagtctcaa gatcttacac
     3421 caccgctgac accagaagag tatttaaccc tcgacaatat acctgcttca cagagcatgc
     3481 ccaaggaatc gaccggacag gcaacacgcc gggtagcaat ccatatacca aataaagatt
     3541 tcaaccctaa gcttgttaag tcgccagagg caactaatac ttgggaattg gagcttcctc
     3601 gaggcacaag tctcgggtta ccagggcggc aaggtggacg gatgtcggcc accgaagtag
     3661 ctcgcgggat caaaggcaaa acttcggctg gaaacagtat cgaaccattt cggcccgaat
     3721 ggatagacta cacaccacat cctaaggtag gaacggactc acagccttta ttacgcagga
     3781 tcaatgggca actaattgaa ccacattacg gcgttttcgg tgcagataat cggcaggtct
     3841 attacccgtc aggttatccc tggacatgta ttgggaagat tctagtgtgg aacgatttct
     3901 cgcagccgtt tcccgcgtgg tcagcctcgg gtgttctgat tggcgggcgc gtcgttttga
     3961 ctgcaggcca tgtggcacct tggggttcga ataattgggc gatgcaattc atcccatctt
     4021 tctacaacgg ggggtcgaca ctgggcgcag gcgtgagttc gtgggtatcc gactatcggg
     4081 gatacaacac gggaaatcat gtctcagcat gggatatgct agtgttgcgc ctctacacac
     4141 ctctaggcaa cacttatggc tacttcggga caaaggacta ccacagttct tggcaaggtg
     4201 gcaattactg gacgcttgcc ggctatccag gtgcaatagc gggtggaaat cgtccatctc
     4261 ggcagatgtg gtggccagtt ctcgacgatg atgctgacgg aagcgccgag gaggtcgaat
     4321 atgaagctga ttcgagcggg ggtaactcag gaggtccggt gtttggattc tggaacggcc
     4381 agccatatgc aattgctaca catagcggcg gatcaaagac aacgttccta tggtggacac
     4441 tcgaagatac aaatgttggc gcgggaggcg gggcgctaaa cagtcttgtt gcttgggcac
     4501 gtagcaactg gccatgatag atttgtaacc atatatgaga gtaaataata ataaaaacgg
     4561 tcacgcctag gcgtgaccgt tttgctttta tacctgcggc aagctgcgcc gcaaaatgtt
     4621 atacatcggc agagatttca ccgcaccctc tacaacacag gtcgcggggt tatctaccaa
     4681 gtacgcagga atacccagcg atttcgtgag caatttatcc acaccgcgca ataaggctcc
     4741 gctgccgcat aatgccacgc cgcgatcgat aatatcagcc actaattcag gtggcgtttt
     4801 ttccagcacg cgacgacctg tttcgataac tgctttgagt ggttcctgca acgcttcaac
     4861 gatctcacct gtggtcagcg tgacagggcg cggcagaccc gtgacttgat cctgcccctg
     4921 cacttccatg ctgttttccg tatcctgtgg aacagcagct ccgattttca ttttcaattg
     4981 ttccgcggta acctgcccca caacgatccc atactttcga cgcacatagt tgacaatcgc
     5041 gtcatcgaga tcaatgccac cggcgcgtaa ggtctccgca gagacgatgc catacatcgc
     5101 aagcacagca gcttcggtgc agcccccgcc aaggcagatg accatgtttc ccgagggcga
     5161 gttgatcggc aggtccacgc ccagcgcggc tgcaagcggt tgttggatca aataggcttc
     5221 gcggctaccc gcttccaaaa cagcttcata cactgcgcgg cgctcaacgc tggtgacccc
     5281 atacgggacc gagatcatca cgcgcggccg aaagattcgc atggatccgc tcacgcgttg
     5341 cagcaaatac gagagaagtg tttcggtgat ttcgtaatcc gcaatgaccc cgttcttgag
     5401 agggcgcgcc acttcaatgc tttcaggaac gcgcccgtac atatcgcgcg cctcctgccc
     5461 cactgccacc attttttgat cagcgacttc aatggcaaca atggtcggtt cctcgagcaa
     5521 tacgcgactg ctatccgcca ggcgtgtgaa cattgtgccc agatcgatac ccaggtcttt
     5581 agaaaagagt gccacggttc ctttaattca gcaaataaat gctttatcta aaatttgatt
     5641 tcagacgaaa atagtgaaag attttgcttg ccaccgcaac atgatgattt cacataccgg
     5701 gaagagcgat cggtgattgt tcatgtgcga ttcccttcgt gaggaggagt acaatcgaat
     5761 caatcatacc tgaaaggcac agcattgtct agttggaaaa gttttttact ccaaaacctt
     5821 gtcaattgaa catcttgtac tatactaccg cttgtttcaa taagaaacag gtagtgggag
     5881 cggctaagag agtgagtgat aattcactta gccaccgaga gcactgagcc ctttgagaaa
     5941 aaactcaaaa actctgtggc ttacacactg aattcgccac tcctcaggta gttgaaatga
     6001 tttgtaaatt agatttcaaa tttcagttta gctaattacc cggcactttg tagttggcga
     6061 aaattttttc aaggagaata gaaaatgctg cttcgcggag atgaaaatcc cctcagtcgt
     6121 cgcatgaaac gatattggaa ggtcgctgtt cttgctaaca tcaaggatga tacccagccc
     6181 aagccggatg gtgtcccccc ggatgccttt gccgacttcg accacatcga aacgattgat
     6241 ttcattcgtg cggccctcga aaccgatggc catcaaacgg tctttatcca cgcggatgca
     6301 gatctcccgt acgcgctccg agatgaaaag cctgatattt gtttcaacat cgcggaagga
     6361 ttaggcggtg acgcacgcga ggcgcaggtg ccagcactcc tggaaatgat gcgcattccc
     6421 tacacgggct cgcgcgtgct gaccaacggc atatccctcg ataaaacatt gacaaaacga
     6481 atctggcgcg accgacgact gccagttgcc cctttccagg agtttgccgc tggcgatgag
     6541 cctttacgcc cggaacttaa ttttccgctg tttgtaaagc cagcccgcga aggcacaggc
     6601 atgggtgtcg acacaaaagc cattgtaaag aatgagaagg aacttcgcga acgggcgatg
     6661 tatatcatcg atacctatca gcaacctgcc ctggtcgaaa cgtttttacc aggtcgtgaa
     6721 ttcactgttg
//
LOCUS       DASTJP010000038        23087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1047812, whole genome shotgun sequence.
ACCESSION   DASTJP010000038 DASTJP010000000
VERSION     DASTJP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 23087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23087
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047812"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1717
                     /locus_tag="VFQ23_01045"
     CDS             <1..1717
                     /locus_tag="VFQ23_01045"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905185.1"
                     /translation="ERLVNAPPQVSSNPYLFMQPFGFGDGYRFFGRQETLPELLNHLN
                     NSTTTFLDGSGKTSLLQAGVIPAVVNEGHLPLLISVSDEPLETSIKKQLLPNIGDMEF
                     LDLMSLTEFVRRVSDHLKGKRLLLLIDQFENIYDQPQTFRNVFTEEWKLCVSGNAPDV
                     HWLFSIPSGSTYLLNMFKEKVSINPNLVTLQPLEREEARQAMAEQANLRDIQIDADVA
                     DTILDDLDRLDNSVIDPAQLQLVCYMLAGGQSTLVKHWTMEHYIGQGRVDGILRGYLD
                     RTIGNLEPVNREPAWQLLAALIDSSESVSNEAELIQKMKQFDVAEQITRDVLSYLEES
                     HLVEYTTAYRLSSDSLRPSIQQWLDQRAAGEKVKAEVSRQVRNIGGSALRGLLGGAVG
                     LMLSYWVLPYEERLPVTDPLFYSWYIYPLSLRAMVGAIAGFFLILLIDLIIASFRGTR
                     NQLRLPAGILAGGSCFAAALLFHMLLHSTGNQFASLLQKIIIEGASWGVVAGAGAMWI
                     MTSTRQIWLKVLTVSIFAGLTLSLSDSLLGGLDVSAPFYIVFISGMVLPLFLLGSALW
                     GKTRI"
     gene            1746..2975
                     /locus_tag="VFQ23_01050"
     CDS             1746..2975
                     /locus_tag="VFQ23_01050"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9905186.1"
                     /translation="MISLLSVLVMTSCNIPVNTPPAENQSIGQIEKLQGGVQAGPASS
                     LTTVESLRDMYNNDAVHVYNNGKAELDFGHGLAFTLYNDTVSGGARVDDGGTSPQAEI
                     RLSEGGLKGYNPPGSETRVKLPNDVNILILGTHYVVAYDPVADETWVYNFDGLLQYEL
                     PPGGYQALSSGTLLKIDKAQMTHLYYGFKFSEQDFDARATSLNSPIQAVREIVRDGLV
                     IPVTGETATPTNTPTATATLTSLPTSTQIPTLTATPIPCYSARFVADVTIPDGSMISP
                     LTFFTKTWRIRNTGSCVWDANYQVVFVGGTQMFAAASNAFPWTAGVVGYGQTADITVS
                     LVSPRLPGMYQGDFMLMAPNGRYFGLGTEHKSFWVRIVVPEPSTPTPSPTPTITPTIS
                     TPWTITPGTLTSGPVVP"
     gene            complement(3140..3556)
                     /locus_tag="VFQ23_01055"
     CDS             complement(3140..3556)
                     /locus_tag="VFQ23_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HET9905187.1"
                     /translation="MSIRNASAVWNGTLKEGNGTMKLGSGAFEGSFTFASRFEEGPGT
                     NPEELVGAAQAGCFSMYLAAQLTDAGYPPAQIRTSAKVHLEAGPRITLIELDTEAEVP
                     NVDEKTFQEKVDYSKANCPISLALTGPELRVSARLV"
     gene            3774..4910
                     /locus_tag="VFQ23_01060"
     CDS             3774..4910
                     /locus_tag="VFQ23_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9905188.1"
                     /translation="MPQIFISYSRKDMDFARKLAGDLEKAGYDVWWDLTDLRGGDDWV
                     RTIPDAISSSQYVIVVLTPDSVESEWVRKEYTQALSLRKKIIPILLKPTGVPFALNTI
                     NFVNFSTGEYEDRFKELLSALGFSGTPPSVTPYQKALLSLPPWLLRFGIPVLLAIFLL
                     ILFLIPRTNPPPDETPTPTATPSLASSPTVTQTITPEPLTSTPTSTSTIAVTPSLTPT
                     RPTFTPSATPPFRSVELCVEVQRSDVQTINVRSGPSSTLYAPLGQIDVGTCLLFSAVH
                     VNPMDETWLLIANNQTNAELMRFAGGWIRSELLGLEPTDSIPLAAVTLTPTPTPSNTP
                     TITPTFTRTPSPTLTDTSTPTSSHTPTPLPTETDTLTPTETPTP"
     gene            complement(4957..6078)
                     /locus_tag="VFQ23_01065"
     CDS             complement(4957..6078)
                     /locus_tag="VFQ23_01065"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET9905189.1"
                     /translation="MLTLSDIAHIRFHNQQIAQTKFKKPDQVIAWLGAMQGQEYAMAK
                     WAIAQRMQEITDSEIDQAFANGSILRTHLLRPTWHLVTPADIRWLLALTGPRVQGVNA
                     YMYRKLEMDSTFFKRSNAALIKALQGGKQLTRLEIASSLQQAGIETAGELRMGYILMH
                     AELEGIVCSGARRGKQFTYALLEERAPHVKSLDRDEALVELVRRYFTSRGPATMKDFV
                     VWSGLTMADVKKGIELLRPKLESAIVEDQTYWFAEVPSLPKKPSGTAHLLPIYDEYVM
                     GYKDRSALLGLLEKENLVGSSIAFDNIIVIDSMLVGSWKRTLSKKEVLVEINFNVPLT
                     KAQKQAVNTDVARYGKFLGLSPVIVKTEKPPPRSREGLL"
     gene            complement(6179..6901)
                     /locus_tag="VFQ23_01070"
     CDS             complement(6179..6901)
                     /locus_tag="VFQ23_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9905190.1"
                     /translation="MSEEFDRRRILVVDDEERMVRFIRMNLEHDGFQVAEAFNGKQAI
                     QKLRDVTPDLILLDVMMPDLDGFEVLETIREGSNVPVIMLTAKGEEDDRVRGLELGAD
                     DYITKPFSPREMVSRVKAVIRRTESAGGSMHDIIEVDDRLKIDFDRREVWLEGKLVKL
                     RPTEYRLLYHLVQNAGWVVTHDQLLVKVWGYEYRDEPHYVRLYINYLRQKLEKDPANP
                     KYILTERGVGYRFVDYKRQKAA"
     gene            complement(6905..7147)
                     /locus_tag="VFQ23_01075"
     CDS             complement(6905..7147)
                     /locus_tag="VFQ23_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905191.1"
                     /translation="MAYSVILHVSGEASILGEIEELPKPTDTIITLTNPRLRDGKDIH
                     YLEQNVVKVIWPLAKITLIEVLESEDEENLIGFVRE"
     gene            complement(7267..7776)
                     /locus_tag="VFQ23_01080"
     CDS             complement(7267..7776)
                     /locus_tag="VFQ23_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9905192.1"
                     /translation="MIICSNCQYKNVSGAVFCAECGAQLDGVETLVTQAITDEQIEEE
                     LKKRSARPEPVSAPSNSWISLHLMDSGKILPLASRNEFTLGRLSEGQPIMPDIDLTPY
                     QAYASGVSRLHAVVKRDNNRVAVMDLGSSNGTYVNGRRLNPHTEEALSHGDIVALGKL
                     KIQILLRNI"
     gene            complement(7773..9257)
                     /locus_tag="VFQ23_01085"
     CDS             complement(7773..9257)
                     /locus_tag="VFQ23_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET9905193.1"
                     /translation="MSSRQPDFYALLGVARDASPEEIKRAYFEAAQRLHPDKNTAAGE
                     TEMFLGVQQAYETLSNPQRRAKYDAILPPEKKLILPYQHSILYSRPNLVQLNEPQMLY
                     IMLELEAPEEARQSSSPPLNVSLVIDRSTSMQGEKMDVVKAAAIQVLRNLRQQDILSV
                     VAFSDRAEVIIPAAFHQDRSRLESKIQMIRPSGATEMYQGLELGVKEVYRSLDAKRVN
                     HIILLTDGHTYGDEQQCLELAQRLSEHKIGVSCMGIGKEWNDIFLEALSTSTGGSTAY
                     IAQPGDIKRLLLEKFDALSQTFAEDVTLQMAPVEGAELTYAFRLHPEPSTIMIENQLH
                     LGAILQDAATSVLFEYIIQPKAVKSDLLTILNGTLKVSISSYPSPVPPLKINLTRPVL
                     NSPEVEPPPPKIVQALSRLMLYRMQERARKEIENGNIDTATRQLQTLASNLLAQGERS
                     LAQTILLEVNNLQHKNTLSEEGSKKIKYGTRALFLESPKKEIIS"
     gene            complement(9260..10180)
                     /locus_tag="VFQ23_01090"
     CDS             complement(9260..10180)
                     /locus_tag="VFQ23_01090"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET9905194.1"
                     /translation="MNKLSRWMSQLFGNNQAAPAAATQEIANTAPLSEQQIQSIVSNQ
                     NPRFDLQQLISACGQSVGKQRDLNEDSLLSITTTMAGNSGNLPFGLYIVADGMGGHQY
                     GEVASNAAIRTVAGYILRKFHPYLFHVKTDTMEESFQEIMQAAVTDAQRAIQREAPGS
                     GTTLTAALVIGQQITVAHVGDSRAYFIYPDGRIEPITRDHSLVKRLEELGHITPDEAA
                     NYPHRNVLYRALGQGEILDPDIFTIGFPQPGYLMICSDGLWGVVAEQDLIRSIVDAPN
                     LQRACQNLVSAANTAGGPDNISVVLAQLIG"
     gene            complement(10194..11996)
                     /locus_tag="VFQ23_01095"
     CDS             complement(10194..11996)
                     /locus_tag="VFQ23_01095"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9905195.1"
                     /translation="MLAKRYAIQEVIGIGGMGSVYRARDMHFPSVTKLVAVKEMINSA
                     PDPLVRETIVQNFEREANLLATLQHPSIPRISDYFTVESRSYLVLEFIHGKDMEAIIN
                     DTNGFLPEDEVLRWAIELCDVLDYLHQHRPDPIIFRDMKPSNVMINHNGDVILVDFGI
                     AKTFQTGIKGTMIGTEGYSPPEQYRGEATPAADIYAMGATIHHALTRRDPRLEPPFSF
                     SERPIRRINSSVSPELETVVNTALEYNPESRFKSSAEMKQALMGVARKTGALVRSPQT
                     GTIQTTGVKPLWSFKCEDEIRGTPTLFQGSLYIGSYDNNLYALGAADGKFQWKYPTDG
                     GIVSRPALLDGNIYVGSEDKRLHVVSARSGKVVWTYFTNGPIRSSPRIAEGHIFIGSD
                     DNHIHAVNINSGRMAWRFETADYVRSTPFVMNDLVYAGCETGDYYAIDFRGELKWRFR
                     AKRAITSSTVGLGQVLYFSSVDGTLYSLDSRNGWVIWRFRLGKASISTPTVVDDFVFV
                     GAADGFIYAVETKSSKEIWRFRTEHQVNGSPVVYKDSLYCGSVDGTLYCLEYRTGRLR
                     WKFGTEGAITGTPLVYDDIVYIGSTDHRVYALLA"
     gene            12312..12938
                     /locus_tag="VFQ23_01100"
     CDS             12312..12938
                     /locus_tag="VFQ23_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9905196.1"
                     /translation="MKILVLSDEVVERLYSLCGSGHFSEIELILGCGDLPYPYLENLL
                     TLLNLPLFYVPGNHDPIYNPNSSLAHAEGGSNLDLKITRYKKFLIGGFGGSIRYRPDG
                     TNQYTQTEAYFRALRLLPRLFLNKMSYGRALDILITHSPPFGIHDDETQSHQGLKAIN
                     RLLRWAKPRYHFHGHTHFQRGNLSPSETTKDLTKVINVFPYKIIEVRH"
     gene            12966..13901
                     /locus_tag="VFQ23_01105"
     CDS             12966..13901
                     /locus_tag="VFQ23_01105"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET9905197.1"
                     /translation="MTSPHPLAGVYAAAVTPLRSASLNGNKSDSILDLESVPGLLNFL
                     ASRGCHGAVLFGTTGEGPSFSPSEREAVMRAASASRDKLPGFRLIAGVGTPSLSETIE
                     LTKLAFDLGYEAALALPPYYFRNASEDGLFDWFSELITKAVPSDGYLLGYHFPKVAGI
                     GFSINLLARLKDAFPIQFAGIKDSSHDADLTRNLGEKFGYDLVVLTGTDSYVQLAMEN
                     KAAGCITAPANIISPDLRTVWDLMQEGKDASAAQAHVTELREILDKYPPFPPTLKAML
                     HRLHGLPRWHVKPPLVEITEATEEQLINELAGIKQ"
     gene            complement(13941..14498)
                     /locus_tag="VFQ23_01110"
                     /pseudo
     CDS             complement(13941..14498)
                     /locus_tag="VFQ23_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
     gene            14637..15902
                     /locus_tag="VFQ23_01115"
     CDS             14637..15902
                     /locus_tag="VFQ23_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET9905198.1"
                     /translation="MTKDLRVNSDRMLASFNEVASIGATGDGGVHRPTFNEAHLAARN
                     WFRQEIEKSGFEFRTDGAGNHSAVLPVTVSSGESLLATTETKTLLLGSHLDSVPNGGR
                     FDGALGVISALEVLKTVKENGIKLKVNLEAMDFTDEEGTLVGLLGSAALGGHLKPEAL
                     QAPRGGRENLVEGMRRASLSEDGMLKAARPKDSLAGYLELHIEQGKRLERAGINIGIV
                     SAIVGIWSYRLSFIGRADHAGTSTMDDRLDASLGASAFTLAARELVMKVFPNCVVNVG
                     KMEFAPGAFNIVPARVEVSLEFRSPDEEQFRRLDSALIARAKAEAQKFSLELKIEDLG
                     SHSPSPMSEVARRAFAEACDDLGLTHVALTSGAGHDAQSLADLCPVGMIFVPSVDGAS
                     HSPREFTKWEDCVNGANVLLHAALKLATE"
     gene            15982..16998
                     /gene="add"
                     /locus_tag="VFQ23_01120"
     CDS             15982..16998
                     /gene="add"
                     /locus_tag="VFQ23_01120"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET9905199.1"
                     /translation="MMKNFDPKYNEIPKTEIHCHLEGAIRTQTIIDIAQQHNLKLPSY
                     EASELDKYVKVYDQLRDLEAVLAAFAIFQNSITSPTVVERIAWELFEDSAKQNIKLFE
                     VRFSPDWAFHGHNLDWDECLEGLLSAKERAEKEFDMAIGYIAITSRSMGPASCVKTVD
                     WAIRHRNNILGIDLADSERDFPLSEFVAPVRKAKEAGLKVTIHTGEDTPASYVRETIE
                     LVNPDRIGHGIHAIDDMKVVDLLKERGITLEVNPWSNYLTNSVRTIEEHPLKKLFDLG
                     VRVTINSDDPEVLETNLNNEYRLAHEVLGMSLAEIALCNRYAYEASFLPENDKKRVWE
                     KYFQ"
     gene            16995..17423
                     /locus_tag="VFQ23_01125"
     CDS             16995..17423
                     /locus_tag="VFQ23_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9905200.1"
                     /translation="MMRVMFPVTIHLFFFRDNQMLLLRRFNTGFRDGEYSVPAGHLDG
                     GETVIEAAAREAAEEVGVQIEAQDIIFSSVMHRLDGEERVDFFVNVHKWQGEPFNAEP
                     EKCDDLRWADVDRLPENLVPYVRRALNNHLNKIQFDEYGW"
     gene            17428..18114
                     /locus_tag="VFQ23_01130"
     CDS             17428..18114
                     /locus_tag="VFQ23_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HET9905201.1"
                     /translation="MGYLVLEGGAEFGGQMREPDLRGIELAGGFDAPIRIIPTAAAPD
                     NNHQRAGNNGIRWFQSLGAKDVLSVSLLSKIRAEDKNIAQALRDAKLIYMLGGFPHYL
                     GQTLLGSLAWDAVLEAYQNGAVIAGSSAGAMVMCQHYYDPDRKRVHEGLNLLPHSLVL
                     PHHNTFGKNWAPQLLKKLPEATLIGIDEQTGMINDGENNSWNVYGRGAVTLYQNEKVT
                     THQAGNSFSV"
     gene            18163..18987
                     /locus_tag="VFQ23_01135"
     CDS             18163..18987
                     /locus_tag="VFQ23_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HET9905202.1"
                     /translation="MFDLASARFAFLDLETTGLSPWFGDRICEVGIVLTEGKRIKQQV
                     QTLVNPERPLSIGAASTNGLTDEELATAPKFANVANVLVEWLRGTVVVCHNAQFDVQF
                     LDSEFRRLGREIQIPNLVDTLMMAREYFDLPSYSLHSIAEAFQVPMTTEHRALDDAHT
                     SRGIFFAMMEEMKKLNKPLDEYIGIYNSPVWPNDGIQLPTELGEAIYSNRKMFIRYVD
                     GEGEETQRWITPKQVMGLSDYVYLKAHCHLRNGERNFRLDRIVEVKVEEWSKKLGL"
     gene            19085..19615
                     /locus_tag="VFQ23_01140"
     CDS             19085..19615
                     /locus_tag="VFQ23_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905203.1"
                     /translation="MNLFDFTHEDLKLNQRGKVSPRQREWLNNTARGIRSMSGMNITI
                     AVGFLFFGLCLILGLYLQNEDTRAALFANPLNLLVFPATILIVAVILTLSVLYMGSLA
                     NRLAAAQVQSAQGKVRLEEEHGEGGTAYYVFVGKKKFAFGEDMSRVFREGEKYKVYYC
                     KSGVYEMVLSYEKVGS"
     gene            19709..22219
                     /gene="recG"
                     /locus_tag="VFQ23_01145"
     CDS             19709..22219
                     /gene="recG"
                     /locus_tag="VFQ23_01145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET9905204.1"
                     /translation="MQPSLEKLRKFFRLEHENGYANSAIIGGLAKMLDYWEGEARADG
                     IQEGVIQAVVQRLRSYDGLTPTSRADALKGLWKRIGETYPEAQQKPKNGAANVENQQK
                     PRPAVAQPAPEFSSKPESSKQDQSQQRQRPIAPPRSESVPGAKTSATPAALDAKLTVL
                     QGVGPRNAESLTKLGMHTLGDMLYYFPRRYDDYSQLKPIKSLWYGETVTVIGTIQSVN
                     TRHIRGGKATIIEVIISDGTASLRLTFFNQPWLGNRFKQGDAISVSGKVDQYLGRLVM
                     NGPDWESVEKESLHTNRIVPIYSLTERITQKWLRGLMKQVIEYWAPNVPDALPESILT
                     SAQVMPLNDALMQIHFPDTQEKIRAARERLAFDEIFYLQMGVLRQKRDWKSVEAKRFP
                     ISDEWLQTRLTSLPFTLTAAQQNAINDIRMDLDSGKPMNRLLQGDVGSGKTVVAAIAA
                     AIITNSGSQVAIMAPTSILAEQHYRSFTNLLKDLINPNEIRLLVGDTPEPEKEVIRAG
                     LADNTIKIVIGTHAVIEGPVQFADLQLAVIDEQHRFGVQQRAELRSKGTSPHLVVMTA
                     TPIPRSLALTLYGDLDISIMNEMPVGRQPINTYVLRPQERERAFTLLRGQIKDGKQAF
                     IIYPLIEESDKIEARAAVDDYETLSKEVFHDLKLGLLHGRMRPSEKDDVMLKFRDKKF
                     DILVSTTVVEVGVDVPNATVMLIEGADRFGLAQLHQLRGRVGRGSAQSYCLLIPTHED
                     ATENERLLAMAESNDGFVLAERDLQQRGPGEFLGTRQAGYTSGLRMASLTDVKLIEKA
                     RTQAQNLFEKDPDLSQPEHALLAEAFGRFWGEGKGDVS"
     gene            22295..22813
                     /gene="coaD"
                     /locus_tag="VFQ23_01150"
     CDS             22295..22813
                     /gene="coaD"
                     /locus_tag="VFQ23_01150"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9905205.1"
                     /translation="MVRALFPGTFDPIHYGHIDIAERASRLFDELVMAVYDKPLKSLM
                     FSPEERMSLVSEAFKNNPKVSVTGYSGLTVEFCRSIGAQVIVRGLRVFSDFEFEFRMA
                     LANHRLAPDIEIVALITAEEHTFLSGTTVREIAMLNGNVSSMVPPHVEAAFKEKVAEL
                     DDSHSFPNSLRD"
     gene            22864..>23087
                     /locus_tag="VFQ23_01155"
     CDS             22864..>23087
                     /locus_tag="VFQ23_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905206.1"
                     /translation="MDILQLIDRLEELFNESKNIPLTRNVMVDEDRMLDIIDQMRIAI
                     PEEVKKAQQLLGQRDRVLAQAQEEANRTLE"
BASE COUNT         5859 a         5889 c         5567 g         5772 t
ORIGIN      
        1 ggaacgactg gtcaatgctc ctccccaggt cagcagcaat ccgtatctct tcatgcagcc
       61 atttggcttt ggagatgggt accgcttttt cggccgtcag gaaaccctgc ccgaattact
      121 caaccatctt aacaacagca ccactacctt tctggatggg agtggaaaaa caagcctgtt
      181 acaagcaggg gtgattcctg ctgttgtgaa cgaaggtcat ttacccctat tgatatcggt
      241 ttctgatgag ccgttggaaa ccagtattaa gaagcaactc ctgcccaaca ttggggacat
      301 ggaattcctt gacctgatgt ctctgacgga gttcgtgcga cgcgtcagcg atcacttaaa
      361 gggcaaacgc ctcctcctgc tcatcgatca attcgaaaac atttacgacc agcctcagac
      421 gttccgcaac gtatttaccg aggaatggaa gttatgtgtt tccggaaacg cgccagatgt
      481 ccattggctt ttcagcattc caagcgggtc cacctacctg ctcaatatgt tcaaggaaaa
      541 ggtgtccatc aatccgaatc tagtcacact tcaacctttg gaacgcgagg aagcacgtca
      601 ggcaatggca gagcaggcaa acctgcgtga tatccagatc gacgcagatg tggcagatac
      661 gattcttgac gatctcgatc ggctggataa ctctgtcatt gacccggcac agttgcaatt
      721 ggtctgttac atgctggcag gtgggcaaag cacacttgtc aagcactgga cgatggaaca
      781 ttacatcggt cagggaagag tggatggaat tttgcgtggc tatttggacc gaaccatcgg
      841 caatctcgaa ccggtcaacc gcgaaccggc ctggcagttg ctggcagcgt tgatcgattc
      901 gtccgaaagc gtatcgaatg aagcagaact cattcagaaa atgaagcagt ttgatgtcgc
      961 cgaacaaatc actcgggacg tcctgagcta tttggaagaa agccatctcg tggaatatac
     1021 aactgcctac agattatcaa gcgacagttt acgaccaagc attcaacaat ggcttgatca
     1081 acgcgccgca ggagagaagg taaaagcaga agtttcgcgg caggtccgca atatcggtgg
     1141 ctctgcacta cgcggtctgt tgggtggagc cgtcggtctt atgctgtcct attgggtttt
     1201 gccctatgag gagagactac cagtcacgga tccacttttc tattcctggt atatctatcc
     1261 cctgtcgcta cgggctatgg tgggtgctat tgcggggttc ttcctcattc tgctgatcga
     1321 tctcattatt gcatcatttc gaggaactag aaatcagttg cgtctgccag caggaatact
     1381 ggctggtggg tcttgcttcg cggcggcgct cctgtttcac atgttattgc attccactgg
     1441 caatcaattt gcatcattgc tgcagaagat cataatcgag ggagcaagct ggggagtggt
     1501 tgctggcgca ggagccatgt ggatcatgac atcgacgcgg cagatttggc tgaaggttct
     1561 gacggtgagt atcttcgctg ggctaacgct ttcgctctcc gactctctgc taggcgggct
     1621 ggatgtatct gcgccgtttt atatcgtctt tatttcagga atggtgctgc ccctcttcct
     1681 acttgggtca gctctatggg gcaaaactcg aatttgaaag gttggataca atgcaacgga
     1741 ctttgatgat ttctctattg agcgtattgg tgatgacgtc atgtaacatt ccagtcaata
     1801 cccctcccgc tgagaatcaa tccataggac agattgagaa acttcagggt ggggtgcagg
     1861 cagggccagc atcatcccta accactgtcg aatctttaag agacatgtac aacaatgatg
     1921 ctgtccatgt ctataacaat ggaaaggcag aactggattt tggtcatgga cttgctttta
     1981 ctctttacaa tgacaccgta agcggaggcg cgcgggtcga cgacggtggc acctcacctc
     2041 aggcagaaat ccgcctgagc gaaggtgggc tgaaaggcta taacccaccg ggaagtgaaa
     2101 ccagagtcaa actaccgaac gatgtaaata tccttatcct cggcactcac tatgttgtcg
     2161 cctatgaccc tgttgcagat gagacatggg tctataactt tgatgggctt ttacaatacg
     2221 aacttccccc gggaggatat caggcactgt cttctggaac attgttgaaa atcgacaagg
     2281 cccagatgac tcatctatat tatggattca agttctccga acaggacttc gatgcacgcg
     2341 ccacttccct caattcaccg atccaagccg tcagagagat tgtgagagat ggactggtta
     2401 ttcccgttac aggcgagact gccacgccaa ccaatacacc cactgcaacc gcgactctca
     2461 cgagcctgcc aacttccact caaataccaa ccctgacagc aacaccaatt ccatgttatt
     2521 cggcgagatt cgtagcggat gtgaccatac cagatggaag tatgatcagt ccattaactt
     2581 tctttacgaa aacctggcga ataagaaata ccgggtcctg tgtctgggat gcaaactatc
     2641 aggtggtttt tgtaggcggc acacagatgt ttgcagctgc gtcgaatgct tttccgtgga
     2701 cagctggcgt cgttgggtat ggccaaactg cagatattac tgtgagctta gtttccccaa
     2761 gattgcccgg catgtaccag ggcgatttca tgttgatggc accgaacggt aggtactttg
     2821 gcttaggcac ggaacacaag tcattctggg taagaattgt agttcctgaa cctagcacac
     2881 ctacaccttc gcctacacct actattacgc cgacgatttc cactccctgg acgatcactc
     2941 ccgggactct cacttctggg cccgtcgttc cgtaaatgac gatgagttcc gcgcagagca
     3001 tggtttaatg ctgaattggt ggtcagactt ctccaaccac caattcagca gattaccgtt
     3061 catttttctg atacttcaaa atattttgta agcgccaatg taagaagtca aaggccgagg
     3121 ttcacgacga cgtttacgat tagaccagcc gcgcggacac gcgcagctcg ggtcctgtca
     3181 gagccagtga gatgggacag ttcgcttttg aataatcaac tttttcctga aacgttttct
     3241 catccacatt tggcacttct gcttctgtat caagttcgat caatgtaata cgcggaccag
     3301 cctccaaatg gacttttgcg ctggttcgaa tttgtgcggg tggatatccc gcatcggtaa
     3361 gttgagcagc caggtacatg gaaaaacagc cagcctgggc agcacccacg agttcttccg
     3421 gattggttcc cggtccttcc tcgaaacgtg atgcaaatgt gaacgagcct tcgaaggctc
     3481 cgctccccag tttcatggtg ccattaccct ccttgagggt cccattccaa acggcagatg
     3541 cattccgaat tgacatttct atctccttat gaatattttc agttgttggt agtgggggcg
     3601 atggataaac attataatcc ctctacaaat gcagcattgg ggttggtaat accttataat
     3661 ctccctgcgt tccctgattg ccctaattca actcagccag agctgttctt gagatcgcct
     3721 tctcaggatg atcggggact gaacatttct caacgctgta acgtctgaaa tatatgccac
     3781 aaatctttat cagctattcc agaaaggata tggacttcgc ccgcaagctg gcaggggatc
     3841 tcgaaaaagc tggctatgac gtctggtggg atctcaccga tctacgcggc ggcgatgatt
     3901 gggtccgcac gatacccgac gcgatttcat cgagtcagta tgtcatcgtc gtactcacgc
     3961 ctgactccgt tgagtcggag tgggtgcgta aggaatatac gcaggccctg tccctgcgca
     4021 aaaagatcat cccgatcctg ctcaaaccaa ccggtgtgcc attcgcattg aacacgatca
     4081 acttcgtgaa cttttcaacc ggtgaatatg aagatcgttt caaagaactt ctatcggctc
     4141 tgggcttcag tggcacgcct ccctcggtca ctccttatca aaaggcgctt ctctcgcttc
     4201 ctccctggct gcttcgattc ggcattcctg tcctccttgc tatttttttg ctgattcttt
     4261 tcctcatccc gagaaccaac cctccgcctg acgaaacccc aacacctact gccacgcctt
     4321 cgcttgcatc ttcgcccacg gtgacgcaga cgatcactcc tgaaccctta acctctacgc
     4381 ctacttcaac gtcaaccatt gccgtcacac ccagccttac gccaactcga cctactttca
     4441 caccatctgc cacaccaccc tttcggtcgg tagagttatg cgtcgaggta cagcgttctg
     4501 atgtgcaaac catcaatgtg cgctcgggtc ccagcagcac cttgtacgcc ccgctggggc
     4561 agatcgatgt aggaacctgc ctgctcttta gtgctgtcca cgtcaatccc atggacgaaa
     4621 cctggctgct gattgcaaat aatcaaacca acgctgagtt gatgaggttt gcaggtggct
     4681 ggattcgctc tgaattattg ggactcgaac caaccgactc gattcccctc gctgctgtga
     4741 cgttgacacc gacacccact ccaagtaata cgcctacgat cactcccact tttacgcgca
     4801 cacccagccc aaccttaacg gatacttcaa cacctacgtc cagtcacacg cctactcccc
     4861 tccctaccga aaccgatacg cttactccca ctgaaactcc tacaccttaa aagtttatat
     4921 ttagacccta acggttttta atagtttatt ttgaagtcag agcaatcctt cgcggctgcg
     4981 gggaggcggt ttttcggttt tcacgatcac cggggacaag ccaagaaatt ttccatagcg
     5041 cgcaacatcc gtgttgacag cctgtttttg ggcttttgtc agcggaacat tgaagttgat
     5101 ctcaaccagg acttctttct tcgaaagtgt acgtttccaa ctgccaacca gcatactgtc
     5161 gatgacaatg atattgtcaa aggcaatact actgcccacg aggttttcct tttctaatag
     5221 acccagcagg gcacttcgat ccttgtatcc catcacatac tcatcataaa tcggcaacaa
     5281 atgcgcagtt ccggatggtt tcttgggaag tgatggaacc tcagcaaacc agtaagtttg
     5341 atcctcaaca atggcactct caagtttggg cctgagtagt tcaattccct tctttacatc
     5401 tgccatcgtc aacccggacc atacgacaaa gtctttcatc gtcgcgggac cgcggctggt
     5461 aaagtatcta cgtaccaatt ccactaatgc ttcatcgcga tccagtgatt tgacgtgagg
     5521 tgctcgctct tccaacaagg cataggtaaa ttgtttgccg cgtcgtgcgc cgctgcatac
     5581 aatcccttcc aattcagcgt gcatcagaat atatcccatg cgcaattcac ccgcggtttc
     5641 aatccctgct tgctgaagtg aggatgcgat ctcgaggcgg gtcaactgtt tgccgccttg
     5701 caaagctttg atgagcgccg cattgctgcg cttgaagaag gtgctgtcca tttccagctt
     5761 gcgatacata taggcattta caccttggac gcgcggacct gtcagtgcca gcagccagcg
     5821 gatatctgca ggcgtgacca aatgccaggt cgggcgaagg agatgcgtac ggagaatcga
     5881 tccgtttgca aacgcctgat cgatttcaga gtctgtaatt tcctgcatgc gttgcgcgat
     5941 cgcccacttc gccatggcat attcctgtcc ctgcatcgcg ccaagccacg caataacttg
     6001 atcaggcttt ttgaatttcg tctgggcaat ctgttggtta tgaaaacgaa tatgtgcgat
     6061 gtctgaaagt gtgagcatga atggattata aacgagcgac tacaccagcc tccaaaaaga
     6121 aaagcggacg acctgagtca tccgctttta gtagtgtctt gaatctaacc tcgaaaagtt
     6181 atgctgcctt ttgtctcttg taatctacaa aacgataccc aacaccgcgt tcggtgagaa
     6241 tatatttcgg gttggcgggg tccttttcca gcttttggcg aagatagtta atataaagcc
     6301 ttacataatg aggttcatcg cggtattcat agccccacac tttgacaagc agttggtcat
     6361 gtgtcaccac ccagcctgca ttttgaacca gatgatacaa caggcgatat tcagtcggtc
     6421 ggagttttac cagtttccct tccagccaaa cttcgcggcg gtcaaaatca atcttaagcc
     6481 ggtcatccac ttcgataatg tcgtgcatcg agccgcccgc gctttccgta cggcggatca
     6541 ccgctttgac acggctgacc atttcgcgtg gactgaacgg tttggtgatg taatcgtcag
     6601 cgcccagctc aagccctcgc acgcgatcat cttcctcacc cttggcggtg agcataatga
     6661 ccggtacatt gcttccctct cgaatggttt ccagcacttc aaacccatcc aggtccggca
     6721 tcatgacatc gagcaagatt aaatcggggg tcacatcgcg gagtttttga atggcctgct
     6781 tgccattaaa ggcttccgcc acctgaaaac cgtcgtgttc aaggttcatc cggatgaaac
     6841 gtaccatcct ttcctcatca tccacaacaa ggatgcggcg acggtcaaat tcttcagaca
     6901 tgaattattc cctcacaaag ccaatcaagt tctcttcatc ttcactttcc agcacttcga
     6961 tgagcgtaat cttggcgaga ggccagatca ctttaaccac attttgttcc aaataatgaa
     7021 tgtccttccc atctcgcagg cgcgggtttg tcagtgtgat gatcgtatct gtaggtttgg
     7081 gtagctcttc aatttctccg agaatggagg cttcaccaga aacatgaagg atgactgaat
     7141 aggccataaa tatctacctt tctaactgac gtacagagta ccgcacttgt gtgtcacatt
     7201 cacttaaagc aaaggatatc gctgcacact attcgaaaat agaactgcaa cgagataact
     7261 atactattaa atattgcgaa gcagaatttg tatctttagc ttacccagtg ctacgatatc
     7321 accatgactg agagcttctt cggtatgcgg gttgaggcgc cgtccattga catatgtacc
     7381 gttggaggat cccaaatcca tcacagcaac ccgattgtta tcacgtttga caacagcatg
     7441 gagacgagaa accccggatg cataggcctg atacggagtc aaatcaatat ccggcatgat
     7501 cggctgacct tcacttagac ggccgagcgt aaactcattt ctggaagcaa gcggcaggat
     7561 tttgccacta tccatgagat gtaaagaaat ccaactattc gatggagccg aaaccggttc
     7621 aggtcgagct gaccgtttct tcagttcctc ttcgatctgt tcatctgtga tcgcctgagt
     7681 gacaagggtt tccacaccat ccaactgtgc gccgcactca gcacaaaaaa ctgctcctga
     7741 gacgttttta tactgacaat tcgaacaaat aatcatgaga taatctcctt ctttggcgac
     7801 tctaggaata atgcacgagt accatattta atcttcttgc tcccctcctc tgagagggta
     7861 tttttatgtt gcaggttgtt cacctcaagc agaatggtct gagccagact gcgttcaccc
     7921 tgcgccaaca aattggaggc gagagtttgg agttgacgag tagctgtatc gatattccca
     7981 ttctcgatct ctttacgcgc ccgctcctgc atacggtaca acatgagacg cgacagtgct
     8041 tgaacaattt tcggtggcgg cggttcaacc tccggtgagt tcaatacggg acgcgtaagg
     8101 ttgatcttca aaggtggaac aggggatgga tacgaagaaa tcgacacttt tagcgttcca
     8161 tttagaatcg tcaataagtc tgattttacc gcttttggct gaattatata ctcaaatagc
     8221 acactagtgg cagcatcttg caatattgcc cccaaatgta gctggttctc aatcatgata
     8281 gtggatggct cagggtgcag tcgaaatgca tacgtcagtt ctgctccttc gacaggagcc
     8341 atttgcagtg tcacatcttc ggcaaacgtt tgagaaaggg catcaaattt ttccaggaga
     8401 agacgcttga tatcaccagg ctgcgctatg taggcagtac ttcctcccgt actggtggaa
     8461 agcgcctcga gaaaaatatc attccattcc ttcccgattc ccatgcaact cacaccgatt
     8521 ttatgctccg aaagcctctg tgccaattcc aggcattgct gttcatcgcc ataggtatga
     8581 ccatcggtta gcaggataat atgattcaca cgtttggcat ccaggcttcg ataaacctcc
     8641 ttgacgccca attccaatcc ctgatacatc tcggttgcgc cggaggggcg aatcatttga
     8701 attttcgatt caaggcgtga acgatcctga tgaaacgcag cagggatgat tacctccgca
     8761 cgatcactga aggctaccac actcaaaata tcctgctgcc tgagattgcg caacacttga
     8821 atggccgccg cctttaccac atccatcttt tcaccctgca tggacgtgga acgatcgatg
     8881 accaggctta cattaagtgg cggtgaggag gattgacgtg cctcttcagg agcctcaagt
     8941 tccagcatga tatagagcat ctggggttcg tttagctgta ctaaatttgg gcggctataa
     9001 agaatgctat gttgatacgg aagaatcagc tttttctcag gcggcaagat tgcatcatac
     9061 ttagcacgac gttgtggatt agagagcgtt tcgtatgcct gttgaacccc tagaaacatt
     9121 tcagtttcac cagcagctgt attcttgtcg ggatgcagtc tctgcgctgc ttcaaagtaa
     9181 gcgcgtttga tttcctccgg agaggcatcg cgggctactc ctaaaagagc atagaaatcc
     9241 ggctgtcgag atgacatgat cacccaatca gctgggcgag aaccacgctg atattatcag
     9301 gcccccctgc agtatttgcc gcactgacga gattctgaca ggcgcgctgc agatttggag
     9361 catcaacaat agaacggatc agatcctgtt cggcaacaac tccccacaag ccatcactac
     9421 aaatcattaa gtaaccaggt tgcggaaaac cgatcgtgaa gatatccgga tcaaggattt
     9481 cgccctgacc cagagcgcga tacagtacat tacgatgagg atagttcgca gcttcatccg
     9541 gggtgatatg tcccaattcc tcaaggcgct ttaccagtga atgatcgcgg gtaattggct
     9601 cgatacgccc atccggatag ataaaatacg cacggctatc acctacatga gcaacagtga
     9661 tttgctgccc gatcacaagc gcggcagtca aagtggtgcc gcttcccggg gcttcacgtt
     9721 gaatggcgcg ttgtgcatct gtcacagccg cctgcatgat ctcttggaat gattcctcca
     9781 tcgtatctgt tttcacatga aacagatacg gatgaaattt tcgtagaata tatcctgcta
     9841 ctgtgcggat cgccgcattg cttgctacct caccatactg atggccaccc ataccatctg
     9901 caacaatata aagaccaaat ggcaagtttc cggaattgcc tgccatggta gttgtaatcg
     9961 acagcagact gtcttcgttt aggtcacgct gctttcccac cgattgtcca caggcagaga
    10021 tcaattgttg aaggtcaaac cgcgggttct gattgctgac aatggattgg atctgctgtt
    10081 ccgagagagg agccgtattg gcaatctcct gagtcgccgc ggcaggggcg gcttgattgt
    10141 taccgaataa ctgggacatc cagcgggata atttattcac aaataactcc ttatcaggca
    10201 agcagggcat aaacccgatg atctgtggac ccgatatata caatatcgtc gtacaccaga
    10261 ggtgtccctg taatggctcc ctctgttccg aatttccagc gcaggcggcc agtacgatat
    10321 tccagacagt aaagagtacc atctactgag ccgcaataga gtgaatcttt atacactacg
    10381 ggtgagccat ttacctgatg ttcagtacgg aaccgccaga tctctttgga gctttttgtc
    10441 tccaccgcat aaataaaccc atctgccgcc ccaacaaaca caaaatcatc cacaacagtc
    10501 ggtgtactaa tggatgcttt gcccaaacgg aaacgccaga tcacccagcc attgcgagaa
    10561 tcgagtgaat aaagcgttcc atccacagat gagaaataga gtacctgtcc caagcctact
    10621 gtggaggatg taatagcgcg cttggcacga aaacgccatt tcaattcccc acgaaaatca
    10681 atggcataat aatcgcctgt ttcacagcct gcatacacaa ggtcattcat cacaaaaggc
    10741 gtcgaacgga cgtaatcggc tgtttcaaac ctccatgcca ttcttccgct attgatgttg
    10801 accgcatgaa tatgattatc atcggagcca ataaaaatat gaccttcagc gatccgtgga
    10861 gaagagcgga ttggaccatt cgtgaaatac gtccatacta ccttgcctga acgggcactg
    10921 acaacatgca agcgtttatc ttctgaacct acataaatat tcccatctag caatgccgga
    10981 cgtgaaacaa ttccaccatc ggtggggtac ttccactgga actttccatc ggccgcgcct
    11041 aacgcataca aattattatc ataagaccca atataaagag atccctggaa aagtgtaggc
    11101 gtgccgcgaa tctcatcttc acacttgaac gaccataagg gcttaacccc tgttgtctgt
    11161 atggtccctg tttgcgggct gcgcaccagc gcgccagtct tgcgtgccac gcccatgagg
    11221 gcttgtttca tctcagccga acttttaaaa cgactttcgg gattgtactc aagagccgtg
    11281 ttgaccactg tttcaagttc gggagagacg gacgaattga tcctccgtat cgggcgctct
    11341 gaaaaagaaa agggtggctc aagccgcggg tcccgtcgcg tcaaagcgtg atggatggta
    11401 gcacccatgg catagatatc tgccgcaggt gtggcttccc cacgatattg ttcgggcggg
    11461 gaatatccct cggtgccgat catggttccc ttgatgccag tctgaaacgt ttttgcgatc
    11521 ccaaaatcca ccaggatgac atcgccattg tgattgatca ttacattgga tggcttcatg
    11581 tcgcggaaga tgatggggtc aggtctgtgc tggtgcaaat aatcgagcac atcacaaagc
    11641 tcgatcgccc accttaacac ctcatcttca ggaaggaacc cgttggtgtc attgatgatc
    11701 gcctccatat ccttgccgtg gataaattcc agcacaagat aggaacggct ctctactgta
    11761 aaataatcgg agatgcgggg aatagatgga tgttgaagag tcgctaataa gttcgcttca
    11821 cgctcaaagt tttgaacaat tgtctcacgc accaaaggat ccggcgcgga gttgatcatt
    11881 tccttcacgg cgaccagctt ggtcacactg ggaaagtgca tatcccgggc acgatacacg
    11941 gaccccattc cgccaatgcc aattacctcc tgaatcgcat aacgcttggc aagcatgaca
    12001 cctgcctgaa gttggcgagg aggggaagag ccttgatcac cccgatcgtc tgataactca
    12061 gggaatatcc ttgtttccag tgggcgtctc caattcagta aattatagtt tgcagtgctt
    12121 tgtttgacaa ccactgaaaa ccaaataaca tttcaatttc gtacatatat aggactttta
    12181 ctgttttctg cccattaatc acgccaatat gccctcttgc caggcaaaat atccataaca
    12241 aaaatcccag gtgccttact cttctgtgtt tcccttgtcg ggaaggtgtt tctgcggcac
    12301 aatatgccac tgtgaaaata ctcgtattga gcgacgaagt ggtcgaacgt ctctacagtc
    12361 tttgtggaag cgggcatttc agcgagatcg agttgattct tggatgtgga gatttacctt
    12421 atccatacct tgaaaacctg cttacgctgt tgaatcttcc cttattctac gttccaggga
    12481 accacgaccc aatctataac ccgaacagtt ctcttgctca tgccgaaggc ggctcaaacc
    12541 tggacttgaa gatcacgcgt tataaaaaat tcctgatcgg cggatttggc ggctccatcc
    12601 gataccgtcc tgacgggaca aatcagtata cccaaaccga agcgtatttc agagcccttc
    12661 gcctcctgcc tcgcttgttt ctgaataaga tgagttatgg acgtgcactg gatattctta
    12721 ttacgcattc ccctcctttt ggtatccatg acgatgagac acaatctcat cagggattaa
    12781 aagccattaa ccgactcctt cgttgggcaa agccacgcta tcattttcac ggtcatacac
    12841 attttcagcg cggcaatctc tctccctctg aaacgaccaa agacctgaca aaggtcatca
    12901 acgttttccc ctacaagatt attgaagtca ggcactaaaa acaaatctga aacatcggag
    12961 aaaaaatgac atcccctcat ccactggctg gagtttacgc tgcagcagtc actcctctac
    13021 gttcagcatc cttaaatgga aacaaatccg attcaatcct ggaccttgag tcagttcccg
    13081 gtttactgaa tttcctcgct tcgcgaggct gccatggagc tgtacttttc ggaaccaccg
    13141 gcgaaggacc ttcattttct ccttctgagc gggaggcagt gatgagggct gccagcgcat
    13201 ctcgcgacaa attgcctggc ttccggttga ttgcaggggt cggcacgccg agtctttctg
    13261 aaaccattga actcaccaaa cttgcttttg atcttggtta tgaggcagcc ctggcactcc
    13321 cgccctatta cttccgcaac gcatcggaag atggactctt cgactggttc agtgaactga
    13381 tcacgaaagc cgttccttca gatggatatt tgctcggata tcactttccc aaggttgcag
    13441 gtattggatt ctcaattaat ttgcttgccc gtttgaaaga tgcctttccc atacagtttg
    13501 ctggcataaa agattcatca catgatgctg acctcacgcg caacctcggt gaaaaatttg
    13561 gttatgacct ggtcgttctc acaggaaccg attcgtacgt tcaactcgcc atggaaaata
    13621 aagctgcagg ctgtatcacc gcaccggcaa atattatttc acccgacctt cgcacggtat
    13681 gggatttgat gcaagaaggc aaagatgctt ccgctgctca agcgcacgtg acagagctaa
    13741 gagaaattct tgataaatac cctccctttc cacctactct caaggcaatg cttcatcggt
    13801 tacacggtct gccgcgctgg cacgtcaagc cgccgctggt tgaaattaca gaagcgacgg
    13861 aagaacagct tatcaatgaa ctggctggaa tcaaacaata agtctggctt gagcctttgt
    13921 ttcaaaatgt ttgagcagac ttagtcgttt tcattcgcaa tctgttccag gacgttcttg
    13981 aaaacctcgt acggttgtgc accgacaatc ccataacgat tattgagcac gtaggtcgga
    14041 acgccggtaa cgccgatctg ataggcccat cgtacctggt ccgctacctg agccgtatat
    14101 ttttcagctt ctacatcccg ttgcatttca tctgcatcca acccaacttc ttctgcagcc
    14161 gagcgcaaca catcccattg actaatgtcc tgtccctctg catagactct gcgaaaaaca
    14221 ttttgatgaa actcattgcc tttgccatgt tcacgcgcaa actcggttgc ctcgtgcgca
    14281 aggcgtgtat tgtagatgcg cggtacggaa acgaattcca taccatagga ctcggcaatg
    14341 gaacgcaaac gttcttctga accattggcc cgcgctcgct ttacatgatc tggcaattcc
    14401 ctgccttcgg gcggcgtatc gaaatacaaa tagaatggac gccattctac atccacgtta
    14461 tgttcttctt ttagcttctc gaccacaccc tggccgacat aacaccaagg tcagatatag
    14521 tcggagaaga tcgtcagttt gaaatccatt gattgttacc tttagctatt atttggataa
    14581 aatatacatg cagatagaca tctgccatcc atcatttctt acacaggaat aagcacatga
    14641 caaaagacct gcgtgtcaat tcagaccgga tgcttgcctc atttaacgag gtagcttcca
    14701 ttggtgcaac gggtgacggt ggtgttcacc gacccacttt taacgaagct catctggcag
    14761 cgcgtaattg gttccgccag gaaatcgaaa aatcgggatt tgaatttcgc acagatggcg
    14821 ccggcaatca ttcggcagtg ttgcccgtca ctgtttcgtc gggcgaatcc ctcctcgcaa
    14881 cgacagaaac aaagactctc cttcttggtt cccatcttga ttctgttcca aacggcggtc
    14941 gcttcgacgg cgcgctgggt gtgatctctg cgcttgaagt cctaaaaacg gtgaaagaaa
    15001 acggaattaa gctaaaggta aatcttgaag caatggattt taccgatgaa gaaggcacac
    15061 tggtcggctt gcttgggagc gcagcgttag gagggcatct caagcctgaa gcgttgcaag
    15121 ccccgcgcgg aggacgcgag aatctagtag agggaatgag gcgggcaagt ttatccgaag
    15181 atggtatgtt gaaggccgcg cgcccgaagg attcgcttgc ggggtatctg gaactacaca
    15241 tcgaacaagg caaacggctt gaacgtgctg gaatcaatat cggcatcgtc tctgcgattg
    15301 ttgggatatg gtcctatcgc ctttcgttta tcgggcgcgc cgatcacgcg ggcacttcta
    15361 ccatggacga ccggcttgat gcatccttag gggcaagcgc gttcacgctg gcggcgcgcg
    15421 aactcgtgat gaaggtcttt cccaactgcg tcgtaaatgt tggcaaaatg gaattcgcgc
    15481 cgggcgcgtt caatatcgtt cctgcacggg tagaggtatc acttgagttt cgttcaccgg
    15541 atgaagagca gttcaggcgg cttgactctg ccttaatcgc gcgtgcaaaa gcagaagctc
    15601 agaaatttag cctggaattg aaaatcgaag atctgggcag ccattcaccc agcccaatga
    15661 gcgaggtagc caggcgcgcc tttgctgaag cctgtgatga tcttggcctg acgcacgttg
    15721 ccctgacatc tggcgcgggt catgatgcgc aatcccttgc ggatttatgt cccgttggga
    15781 tgatcttcgt cccgtcggta gatggagcga gtcactcccc gagggaattc acaaaatggg
    15841 aggattgcgt caacggcgca aatgttctac tgcatgcggc cttgaaatta gcaacggagt
    15901 aaaataaaaa cacaaaggac acaaagatca cgaaggaaaa cctttgagta ctttgtgccc
    15961 ttcgtgttta aaaggtagtt catgatgaaa aacttcgacc ccaaatacaa cgagattccc
    16021 aaaaccgaaa ttcactgtca tctcgagggt gccatccgca cacagacgat cattgatata
    16081 gcgcaacagc ataacctgaa gttaccttca tacgaagcca gcgaattgga caaatatgtc
    16141 aaagtatatg atcaattgcg tgacctcgaa gccgtgctgg cagcctttgc cattttccag
    16201 aacagcatta cttctccaac cgtcgttgag cgcattgcct gggaattatt cgaagattct
    16261 gcgaaacaaa acattaaact attcgaggtc cgcttctcgc cggattgggc gtttcacggt
    16321 cataacctgg actgggatga atgccttgaa ggtctcttaa gcgccaaaga acgcgccgaa
    16381 aaagaattcg acatggcaat tggctatatc gccatcacct cgcgaagcat ggggccggca
    16441 tcgtgtgtca aaacggtgga ttgggcgatc cgtcatagga ataatattct tggcattgac
    16501 ctcgcagaca gtgaaagaga ttttccatta agtgaatttg ttgcacccgt tcgaaaagca
    16561 aaagaagctg gcttgaaagt aaccattcac accggcgaag acacacctgc atcttatgtc
    16621 agagagacaa ttgaactcgt gaaccctgac cgtattggtc acggcattca cgccattgat
    16681 gatatgaaag ttgtagatct actcaaagag cgcggcatca ccctcgaggt caacccgtgg
    16741 agtaattacc tcacgaactc agtacgaaca attgaggaac atcctctgaa aaagcttttt
    16801 gaccttggcg ttcgcgtgac gatcaattcg gatgacccag aggttcttga aaccaaccta
    16861 aataatgaat atcgccttgc gcatgaggtg ctgggcatga gcctcgctga aattgctcta
    16921 tgcaatcgtt atgcgtatga ggcatctttt cttccggaga acgacaagaa aagggtctgg
    16981 gagaaatatt tccaatgatg cgtgttatgt tcccggtgac gatacatttg tttttctttc
    17041 gagataacca gatgttgtta ttacgacgtt tcaatacggg ttttcgcgac ggcgaataca
    17101 gcgtcccggc cgggcatttg gatggaggag aaaccgtcat cgaggccgcc gcgcgtgaag
    17161 cggcggaaga ggtgggcgta caaattgaag cgcaggacat aattttctct tctgtcatgc
    17221 atcgcctgga tggggaagag cgcgtcgact tttttgtcaa cgtccataaa tggcagggcg
    17281 agccattcaa tgccgagccg gagaaatgtg atgacctgcg ttgggcggat gtggatcgtt
    17341 taccagagaa tttggtccct tatgtgagac gggcattgaa taatcatttg aacaaaatac
    17401 agttcgacga atacggatgg taaagagatg ggttacctgg ttcttgaagg aggcgcagag
    17461 ttcggtggtc agatgcgcga acctgacctg cgcggcattg agctagccgg cggcttcgat
    17521 gcgccgattc gcatcatccc tactgcagcc gcgcccgata acaaccatca acgcgccggg
    17581 aataacggta tccgttggtt ccagagttta ggggcgaagg atgtactttc agtatcgctg
    17641 ctaagcaaga tacgagccga ggacaaaaac atcgcccagg ctttgcgcga cgcaaagttg
    17701 atctatatgc tgggcggatt tcctcactat ctggggcaga ctctcctggg aagcctggcc
    17761 tgggatgcag tcttggaagc ctatcaaaat ggtgcggtca ttgctgggag cagcgccggc
    17821 gcgatggtga tgtgtcagca ttattatgat cctgatcgga aaagagttca cgaaggccta
    17881 aacctcctgc ctcactcgct cgtcctgccg catcacaata catttgggaa aaactgggcg
    17941 ccacagcttt tgaaaaaact tcctgaggca accctgattg gaattgacga gcaaacgggg
    18001 atgatcaacg atggcgagaa taattcgtgg aatgtttacg ggcgtggtgc cgtcaccttg
    18061 tatcaaaatg agaaggttac aactcaccag gcggggaact cgttttcggt ttaaaattga
    18121 ggtgacagtc atttttagaa ataactgcta cctatacctt ttatgtttga tcttgccagc
    18181 gcccgctttg cttttttgga tcttgaaaca acgggacttt ccccgtggtt tggggatcgc
    18241 atttgcgaag tgggcatcgt gctgacagag gggaaacgca tcaagcagca agtccagact
    18301 ctcgtcaacc ctgaacgccc cctctcgatc ggagccgcca gtaccaacgg tctcacggat
    18361 gaagagcttg ccaccgcccc aaaattcgca aacgtggcta atgtcctcgt ggaatggtta
    18421 cgagggacag ttgtcgtttg tcacaacgca cagttcgatg tgcaatttct cgatagtgag
    18481 ttccgccgtt tgggacgcga gatccaaatt ccaaatcttg tagacacctt gatgatggcg
    18541 cgtgaatatt tcgatttgcc atcctatagt ttgcattcca tcgccgaggc cttccaggtg
    18601 ccgatgacga ccgaacatcg tgccctggat gatgctcaca cctcacgcgg catctttttc
    18661 gcgatgatgg aagagatgaa gaagctcaac aagcctctcg atgaatatat tggcatttac
    18721 aattcgcccg tctggccgaa cgacggcatt caactcccca ccgaattagg cgaagcaatc
    18781 tacagcaaca ggaaaatgtt catccgctat gtggatggcg aaggcgaaga gactcaacgc
    18841 tggatcacac ccaaacaggt gatgggcttg tctgattatg tttatttaaa agcgcactgt
    18901 cacctgcgaa atggggagag aaacttcagg ctggatcgaa ttgtggaagt gaaagtagag
    18961 gagtggtcaa agaaattggg actttgaaac taatgccttt tacttcaatt cggtggtttc
    19021 gatacgccct ttgctgttgc ctcgggctac tcaaccacca ggtaaacaaa attactcttg
    19081 ttaaatgaat cttttcgact tcacccatga agacctaaaa ctaaaccaac ggggaaaagt
    19141 cagcccacgc cagcgagaat ggctaaacaa taccgcgcgc ggcattcgaa gtatgtccgg
    19201 gatgaacatc accatcgccg tcggtttttt gttttttgga ttgtgcctga ttctgggact
    19261 atacctgcaa aacgaggata cgcgtgcggc gttatttgca aacccgctca atctgcttgt
    19321 ttttcctgcg acgattctta ttgttgccgt aattctcacg ttgagcgttc tatatatggg
    19381 cagtctggca aaccgcctgg cggctgccca ggttcaatct gctcagggca aggtccgcct
    19441 agaggaggaa catggggaag gcggtactgc ttactatgtg ttcgtcggga aaaagaaatt
    19501 tgcgtttggg gaagatatga gccgcgtttt tagagagggt gaaaaatata aagtctatta
    19561 ttgcaagtct ggggtgtatg aaatggtgct gtcttatgaa aaggtgggaa gttaaaacag
    19621 agattttgaa gccaaccagc atttcattct gtgaaggggt cgagtgtact ttagaaatct
    19681 tatcctttcc gcatgataaa atcaacttat gcaaccttct ttagagaaat tacgtaaatt
    19741 tttccgtctc gaacacgaga acggatatgc aaacagcgct attatcggtg ggcttgcgaa
    19801 gatgctcgat tattgggaag gcgaagcccg tgcggatgga atccaggaag gggtcatcca
    19861 ggcggttgtt cagagactcc gctcgtacga cgggcttacg cccacctccc gcgcagatgc
    19921 cctcaaagga ttgtggaagc gcatcgggga aacctatcct gaagcacagc aaaagcctaa
    19981 gaatggcgct gcaaacgttg aaaatcaaca gaagccgcgt cccgcggtgg ctcaacctgc
    20041 acctgagttt tcctccaaac ccgaatcatc gaaacaagac caatcccagc agagacagcg
    20101 cccaattgcc ccgcctcgtt cagaaagcgt gccaggtgca aagacctctg caactcccgc
    20161 cgcgctggac gcgaagctaa cagttctcca gggcgtaggt cctcgcaatg ccgaatcgct
    20221 tacgaagctg gggatgcata cgcttggcga catgctttat tacttcccgc gccgctatga
    20281 tgattattca caactcaagc cgatcaagtc gctttggtat ggcgagactg tgaccgtgat
    20341 cggcacgatc caaagcgtga acacgcgcca tattcgcggt ggcaaggcga cgatcatcga
    20401 agtcatcatc agtgatggca cggcttccct gcggctgacc tttttcaacc agccctggct
    20461 tggaaatcga ttcaaacagg gcgacgcgat ttctgtttcc ggaaaggtgg accaatatct
    20521 cggcagattg gtgatgaatg gtcccgattg ggaatcggtt gaaaaggaaa gtcttcacac
    20581 caatcgaatc gtgcccatct attcccttac tgaacgcatc acgcaaaaat ggctgcgcgg
    20641 actgatgaag caggtcattg aatactgggc accgaacgtc ccggatgccc tgcctgaaag
    20701 catactcacc tccgcgcagg tcatgccgtt gaacgatgca ttgatgcaga ttcacttccc
    20761 ggacacgcag gagaagatcc gggcggcgcg tgaacgcctc gcctttgatg aaattttcta
    20821 tctgcaaatg ggtgttcttc gccagaaacg ggattggaag tcggtggagg caaagcgctt
    20881 tcccatctcg gacgagtggc tgcagacgcg tttaacaagc ttgcccttca cgctgacagc
    20941 ggctcagcaa aacgcgatca acgacatccg catggacctt gactcgggca aacccatgaa
    21001 ccgactctta caaggcgatg tgggttcagg gaaaacggtc gtagcagcga tagcggctgc
    21061 cattattaca aacagcggct cacaggttgc catcatggcg cccacctcga tccttgccga
    21121 acaacattat cgaagtttta ccaatctcct caaagatctg attaacccga acgagattcg
    21181 tttattggtt ggcgatactc ctgaaccaga aaaggaagtc atccgtgccg ggctggcaga
    21241 taacacgatc aagatcgtga tcggcacgca tgctgtgatc gaaggtccgg ttcaatttgc
    21301 cgacctgcaa ctggctgtga tcgacgagca acaccgcttt ggcgtgcaac agcgtgccga
    21361 attaagaagc aaaggcacga gtccacatct ggtggtgatg actgccactc ccattccgcg
    21421 ctccctcgcg ttgaccctct atggcgacct cgacatatcc atcatgaatg aaatgccagt
    21481 cgggcgccag cctatcaaca cctatgtatt acgtcctcag gaacgtgagc gcgcatttac
    21541 gctcttgcgc ggtcagatca aagatggcaa acaggcgttc attatctatc cgctgatcga
    21601 agaaagtgac aagatcgaag ctcgcgccgc ggtagatgat tacgaaactc tctcaaagga
    21661 agtgtttcac gatcttaaat tgggtctttt acacggacgc atgcgcccca gcgaaaaaga
    21721 tgatgtgatg ctcaagttcc gggacaagaa atttgacatc ctggtttcga cgactgtcgt
    21781 tgaagttggc gtggatgtcc ccaatgcgac cgtgatgctc atcgagggcg cagaccgatt
    21841 tggcctggct cagctgcatc agttacgcgg acgcgtagga cgcggctctg cccaatccta
    21901 ttgtttgttg atacccacgc acgaggacgc gacagagaac gaacgtttgc tggcaatggc
    21961 agaatccaac gatggttttg ttctggcaga gcgcgacttg cagcaacgcg ggcccggaga
    22021 atttttgggt acgcgccagg ctggctatac cagcgggctc cgcatggcga gtctcacaga
    22081 tgtcaagttg atcgagaagg cgcgcacaca ggcgcaaaat ctgtttgaaa aagaccctga
    22141 cctgagtcaa cccgaacatg cgttgttggc agaagccttt ggacgattct ggggtgaagg
    22201 caaaggggat gtgagttagt caaaaacgtg accaataaac tccgcaggtg ttcgacaact
    22261 atgagactaa aaacgaggag actatcagac taatatggtc agagctttat tcccaggaac
    22321 gttcgatccc attcactacg gacatatcga tatcgcagaa cgggcctcgc gtctctttga
    22381 tgaattggtg atggctgttt atgataaacc cttgaaatcc ctgatgttct ctcctgaaga
    22441 gcggatgtca ctggtaagtg aggcatttaa gaacaacccc aaggttagtg tcaccggcta
    22501 tagcgggcta accgtggagt tttgtcgcag tattggtgcg caggtcatcg tgcgtggact
    22561 acgcgtgttt tcagattttg aattcgagtt ccgcatggca ctggcaaacc atcgccttgc
    22621 gcctgatatc gaaattgtgg cgctgatcac cgccgaggaa catacatttc tctctggaac
    22681 aactgtgcgc gaaattgcca tgttgaacgg taacgtatcc agcatggtcc caccccatgt
    22741 cgaagcagcg ttcaaggaaa aagtggcaga attagatgac tcacactcat ttcctaattc
    22801 gcttcgcgat taattgtgct agaatagctt taataaccag acctcctaat ggaggagtaa
    22861 tttatggaca ttctgcaact aatcgaccgc ctcgaagagc tcttcaatga gagcaaaaat
    22921 attccgctca cccgcaacgt gatggtggat gaagatcgca tgctggatat catcgaccag
    22981 atgcgcatcg ccatccctga ggaagtgaaa aaggcacagc agttactcgg tcagcgcgac
    23041 cgggtccttg cgcaggcaca ggaagaagcc aatcgcacgc tggagat
//
LOCUS       DASTJP010000039         8605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_202193, whole genome shotgun sequence.
ACCESSION   DASTJP010000039 DASTJP010000000
VERSION     DASTJP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202193"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFQ23_01160"
     CDS             complement(<1..197)
                     /locus_tag="VFQ23_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905207.1"
                     /translation="MPHKKAEHNDRMQNRASLTRFAWLSIGAAVATISLKTVAYLLTG
                     SVGLLSDAIESLVNLAGAVMA"
     gene            complement(274..522)
                     /locus_tag="VFQ23_01165"
     CDS             complement(274..522)
                     /locus_tag="VFQ23_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905208.1"
                     /translation="MNNISSFKKKFPWRLMAFLAMGVTVAALAPYATFNPDNFNNATS
                     RFAGETPLRLAGLYIHAFGGGIALIIGHSQLHIQSMTY"
     gene            complement(534..989)
                     /locus_tag="VFQ23_01170"
     CDS             complement(534..989)
                     /locus_tag="VFQ23_01170"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HET9905209.1"
                     /translation="MIDNSLMAFLHHLFAFTLVACVVYEFIAYRKSLTLEEARRIQRA
                     DLMYGISAGLLLVVGFLRVVYFEKGWAFYSQSPFFWLKMNTFLITGLLSIDPTIRYLR
                     WNKTVKENKAPEISDGEFKRTRIILWLEVIGLVIILLAASLMARGVGVN"
     gene            complement(1040..1591)
                     /locus_tag="VFQ23_01175"
     CDS             complement(1040..1591)
                     /locus_tag="VFQ23_01175"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HET9905210.1"
                     /translation="MNKIFNGRYTARTDQPFVVFLIGMRINKWWRIDQWLPVSNAMTP
                     MMQTLFTNPAKGFLHAEFFWNFSGPVLIQYWRSFEDLENFARNPVDPHLGPWKKFNQT
                     VGANGIVGIWHETYTVNPDQFESVYGNMPKFGLAAATEHVPAVGRRETARLRLASNKK
                     HDELTLEKSFDRKATPIREAIKN"
     gene            complement(1594..2283)
                     /locus_tag="VFQ23_01180"
     CDS             complement(1594..2283)
                     /locus_tag="VFQ23_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9905211.1"
                     /translation="MVRPIKKKQIPNLQDAIKETAWKQIAEYGAPALSLRAIARELKI
                     TAPAIYNYFPDRDALVTALIIDAFNSFGDSQIEARESIPAANLGGRFKAIGLAYRNWA
                     HTYPQRYQLIFGTPIPGYQGPPEKIFPSSARSISALFHVVESFRAAGKLNLTSSPPIQ
                     DEYKAYYEMWKAQIGDIHPVSVFVAMIIWARVHGVVSLEIQGNLPPFGKKGDALYLAE
                     LNAIARQFIKE"
     gene            2537..3700
                     /locus_tag="VFQ23_01185"
     CDS             2537..3700
                     /locus_tag="VFQ23_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HET9905212.1"
                     /translation="MRKSLRYLSTFASIIVLLVVVVGSASAAPPAPQAKWTVMVYISG
                     DNNLEEYVVKDIELELAPTGSSANVQVVALADRGPGYDTSRGDWQTTKLYHVTPGMIA
                     DSASAVADWGERNMGDKQTLIDFVTWSKTNYPADHYLLYFWGHGWSWHPGWVMEDDTN
                     ADTLDYEETKAAIPSLGFIDVVGYDGCNMASLEIMKLWQGHATALTGSQEWVGWDGIE
                     YDVVLAQLAANPNMTADQVAIATSQSTVTDKTWSAVAVDARLTTLMTAVDQWATALKN
                     GLPANKKKYDTAFGATRSFWQAPMDKDLYDLAFEINRNVTDTNIKTKSQAVMTAFASV
                     VLHERHVSQYSDAHGITIYGPHRSNQKLDYAYFLTLELAQQTQWDEFLSAYVP"
     gene            complement(3775..5019)
                     /locus_tag="VFQ23_01190"
     CDS             complement(3775..5019)
                     /locus_tag="VFQ23_01190"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9905213.1"
                     /translation="MRRLILFLMLPLLACTGTGTTPPSTPTLEPVTETSASPTAISTI
                     SAPSEIAPTQQPPTLPGVTAFPDPNAYVWQPVASGLDRPVDLQPDGSGNLYLVEKLGH
                     VHLFQDAEDGNPSETPFLNIEDRVNDQSNEMGLLGLAFHPNYSENGFFYVNYTGRGGN
                     TFISRFQSNGGATVDPNSEAILLTVDQPFPNHNGGAMVFGPDGYLYMGLGDGGAGGDP
                     AGNAQSLETLLGKILRIDVDAGDSYTIPADNPFGNEIWHYGLRNPWRMSFDKLNGDLY
                     IGDVGQNAWEEIDFLPAGSPGGANFGWDQFEGNHEYEDAGREAEYIFPIAEYSHPDGG
                     CSVTGGYVYRGSMPEWNGIYLYGDYCTGFVWGMIKPETGGEWQNQLLFQTGQRITAFG
                     QDETGELYLITDGGEILKLAQN"
     gene            complement(5026..5364)
                     /locus_tag="VFQ23_01195"
     CDS             complement(5026..5364)
                     /locus_tag="VFQ23_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HET9905214.1"
                     /translation="MAEKKKIVVPQGGMMRDMVMRLKLITRLMGDRRVSFFVKFLPLA
                     SLAYLFWPIDAIALPIIGALDDAAILWIGSTLFVELCPTNVVNEHMQDLALSEGGEVV
                     DAETTDVDNQ"
     gene            5589..7628
                     /locus_tag="VFQ23_01200"
     CDS             5589..7628
                     /locus_tag="VFQ23_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9905215.1"
                     /translation="MQRRRMSIALFCLGVFIMACTFGGLVPSLSNETPTSAPNVTDLP
                     ATQTVSQTGDMDATLDRLGGQPCVEKPEFTCVTIQVPLNHFDSSNTETLDIVFAVLPA
                     SGERIGMYVQAFPGGPGGEGISNATTVFYPDEILEHFDLVFFDQRGIGLSNPLACPNA
                     YADDFLGYLNATDQAGSEGLDTPEEQQAAIASARTFVETCVAEIGIEPAKLSFYGTDQ
                     VAEDLESFRQTVGDEKFWLYGVSYGTAVAQTYAAAHPEHLAGLILDGTIDMTLNGEEG
                     ALAQEKAFDKVLLAVLEACDQDEACAAELGGDALSVYDNLAAQVSENPISYELPLPSG
                     EKLSKTFTFNQLEFTTAYQMYSLGGRMLFLRALAAAQDGDMVPLARLLHQQASLDPAT
                     GAYLGDPTFSDTMFYSVNCTDDSYYTGTPDERIAQVIEEGQNSNGTVPRLDGSVYTGL
                     SCAFWPSAPQDAVDREPLIAEGVPTIVLNATLDPATPFEQGKTVFERLDNGYHLYVEG
                     GLHSIFGYGETCPDDYVTDFMVRGQLPSEREIVCSWDPAVINAYVPLMPKDVSAFADP
                     LEIFSAIDSELSLQPEYYYSYFTEDTTFACPYGGAFTFGPSDAGESYNFTDCVFTNGF
                     IMTGTGSFDYSSGIFRLETQVSGEKTGTLTYTHDYRTGEVSVTGDYGGETIDLQQ"
     gene            7647..8102
                     /locus_tag="VFQ23_01205"
     CDS             7647..8102
                     /locus_tag="VFQ23_01205"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9905216.1"
                     /translation="MPYLVDGHNLIPKLGLRLDSMDDEMELIAILQEFCRVERKQIVV
                     YFDGAPQAGTRKLGPVTAHFVKLGTTADDSIRRHLKKLGKAAKNWTVVSSDRQVQAET
                     RAVRAEIISSDGFAKQLRQARHAAPKSDEARTLSPDEIEEWLKLFKRNG"
     gene            8121..>8605
                     /locus_tag="VFQ23_01210"
     CDS             8121..>8605
                     /locus_tag="VFQ23_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HET9905217.1"
                     /translation="MSTIYSYVPSPNHIYPDHPERPARLDILEPRLKSFAAESIEAKP
                     AAHQEVEYVHHPKLVSGLEKVCLEDAPGIIDYAPTYVTPSSFHDALLAVGGVIACTRA
                     VMNGDAKNAFAIVRPPGHHAEPDRAMGFCLFNNVAIGAQYALAHGLERVAVIDYDAHH
                     G"
BASE COUNT         2128 a         2109 c         2203 g         2165 t
ORIGIN      
        1 aatgccatca ccgcccctgc cagattgacc agcgattcaa ttgcgtcgga gagcaacccc
       61 actgacccgg tcagcaggta ggctaccgtt ttgagtgaaa tggtcgcgac agccgcgccg
      121 atggataacc aggcaaatcg agttaatgag gcccgatttt gcatccggtc attatgttcc
      181 gcttttttgt gaggcatata tggaatgata catgcgagga acgaacttgc acagagacga
      241 atctccccca ttaggaaagt ctaaaaaaat atttcagtag gtcatagatt gaatgtgtaa
      301 ttgagaatgt ccaatgatca gggctatgcc acctccgaac gcatggatgt acaacccggc
      361 caggcgcagc ggcgtttccc ccgcaaagcg ggaggtggca ttgttgaagt tatccgggtt
      421 gaaggtggca tagggtgcca gggcagcgac tgttacaccc atggcaagga atgccatcag
      481 acgccaggga aattttttct tgaatgagct gatattgttc atggcgaaac ttctcaattt
      541 actcccacac cgcgggccat gagcgacgcc gcaagcagga tgatgacaag cccaatcact
      601 tccaaccaga gaatgatgcg cgttcgcttg aattccccgt cgctgatctc aggagctttg
      661 ttctctttga cagttttatt ccaacggagg taacggatgg tgggatcaat ggataacaag
      721 cccgtgatga gaaacgtgtt catcttcaac cagaagaatg gactctggct gtaaaacgcc
      781 catccttttt cgaagtagac cacacgtaaa aatcccacga ccaacaagag acctgccgag
      841 atcccgtaca ttaagtcggc gcgttggatg cggcgcgctt cttccaacgt caggcttttg
      901 cggtaggcaa tgaattcgta cactacacac gccaccagtg taaatgcaaa taggtgatgc
      961 agaaatgcca tcagggagtt gtcgatcatt ttatttcctt ctttccagga cttttacgtc
     1021 taccatctat atcacttgat tagttcttaa tggcttcgcg tattggcgta gctttcctat
     1081 cgaatgattt ctctaaagta agttcatcat gcttcttatt cgacgccagt ctcaggcgtg
     1141 ccgtctctct gcgcccgact gcagggacat gctccgttgc cgccgccagc ccaaacttcg
     1201 gcatgttgcc gtagaccgat tcgaattgat ccgggttcac ggtatacgtc tcatgccaga
     1261 taccgacgat tccattggca cccaccgtct gattgaattt cttccacggt cccagatgcg
     1321 ggtcaactgg attacgcgcg aaattttcca ggtcttcaaa ggaacgccag tattgaatca
     1381 ggacgggacc actgaaattc cagaagaatt cggcatgcag aaatcctttg gcggggttag
     1441 tgaataaagt ctgcatcatg ggcgtcattg cattggaaac cgggagccac tgatcaatcc
     1501 gccaccattt gttgatgcgc ataccgatca aaaagacgac aaagggctgg tctgttcttg
     1561 ccgtataacg accgttgaaa atcttattca tgtctactcc ttgatgaact gccgggcaat
     1621 ggcattcaat tcagccagat acaaggcgtc accttttttg ccgaaaggtg gaagattgcc
     1681 ttgaatttcg agggaaacga cgccatgtac gcgcgcccat atgatcattg ccacgaaaac
     1741 ggatacggga tgaatatcac cgatctgcgc tttccacatt tcataatatg ctttgtattc
     1801 gtcttggatg ggaggcgagg acgtgaggtt gagtttcccc gccgctcgaa aactttcaac
     1861 gacatggaag agggcgctga tggagcgtgc cgaagatggg aatatcttct cgggtgggcc
     1921 ctgataccca gggatgggtg tcccaaagat aagttgatat cgctgcggat aggtatgcgc
     1981 ccaattgcga tatgcaaggc caattgcctt aaatcgcccg cccagattcg ctgccggtat
     2041 agattctcgt gcttcgatct gggaatcacc gaaggagtta aacgcgtcga tgatcagcgc
     2101 ggtgacaagg gcatcgcgat cagggaagta attgtagata gctggcgcag tgatcttgag
     2161 ctcacgcgct atggcacgca gcgaaagggc aggggcgcca tattctgcaa tctgtttcca
     2221 ggctgtctct ttgatggcat cctgcaggtt tgggatttgt tttttcttaa tgggtctgac
     2281 cataggatct ccaattaact ttacgctgta aatatacagt gtaaagttat atttgtcaag
     2341 ggcagccgtc ggtggttgag gggcgaagcc cgtgtcgaaa ccacagaagt aatcatgaac
     2401 tacaattctc tcaatgaatg attactacga tgacatcatc aatgtaacaa attgattctt
     2461 gaatccgttt tatagttatg ctacactctc aatcgaaaga gatcgtatcc acctcatatc
     2521 tccaaatgga ggaaccatgc ggaagtcgtt acgttatttg agtacgtttg catccatcat
     2581 tgtgttgctg gttgtcgtcg tggggagcgc ctccgcggca ccacctgccc cgcaggcaaa
     2641 atggacagtg atggtttata tctcagggga taacaatctc gaagagtacg ttgtcaaaga
     2701 tatcgaattg gaactcgcac ccacggggtc aagcgcgaac gtgcaggtgg ttgcactggc
     2761 agatcggggt ccgggctatg atacgagtcg cggcgattgg cagacgacga aactatatca
     2821 tgtgactccg ggcatgatcg ccgactctgc aagtgccgtt gcggattggg gtgaacggaa
     2881 catgggtgat aaacaaacac tcatcgattt cgtcacatgg tccaaaacca actatcccgc
     2941 tgatcactat ctgctgtatt tctggggtca cggctggagc tggcacccgg gctgggtgat
     3001 ggaagatgac accaatgcag atactcttga ttatgaggaa acgaaagccg ccatcccctc
     3061 acttggattt attgatgtgg ttgggtatga cggctgcaat atggcatcac tcgagattat
     3121 gaaactctgg cagggacatg ccaccgccct gaccggttca caggaatggg tgggttggga
     3181 tgggattgaa tatgatgttg ttcttgcaca attggctgcc aacccaaata tgactgctga
     3241 tcaggtggcg attgccacaa gccagagcac cgtcaccgat aagacctggt ctgccgtggc
     3301 tgtggacgca cgtctgacca cgttgatgac agccgtggat caatgggcga ctgcattgaa
     3361 gaatggctta ccagcaaata agaaaaaata tgatacagcc tttggcgcca cgcgcagttt
     3421 ctggcaggcg ccgatggata aggacttgta tgatttggcg tttgagatta atcgtaacgt
     3481 caccgataca aatatcaaaa caaagagtca ggctgtcatg acggcctttg cgagcgtcgt
     3541 cttgcatgaa cggcatgtat ctcaatacag tgacgcgcac gggatcacaa tttatggtcc
     3601 tcatcggtca aatcagaaat tggattacgc ctacttcctt acgcttgagc tggcgcaaca
     3661 aacccaatgg gatgagttcc tcagtgcgta tgtgccatag ccatagtggg ctttgaaaaa
     3721 taaaaaatga ccgccgatga attatcggcg gtcatttttt gatcttgaaa ggatttagtt
     3781 ttgtgcgagt ttaaggatct ctccaccatc ggtgatgaga taaagctcgc cggtttcatc
     3841 ctgcccaaag gctgtgattc tttggccggt ctgaaacagt aattgatttt gccattcgcc
     3901 gcctgtttcc ggtttgatca taccccagac gaagcctgtg caataatctc cgtagagata
     3961 gattccattc cactcaggca tggagccgcg atagacatag ccgccagtga ccgaacagcc
     4021 gccatccgga tggctatatt ctgcgatggg gaaaatgtat tcggcttcgc gcccggcatc
     4081 ttcatattca tgattgcctt cgaattgatc ccagccaaag ttcgcgccgc cgggagagcc
     4141 cgccgggagg aaatcgatct cctcccatgc attttgcccg acatcgccaa tatagagatc
     4201 gccgtttaat ttatcaaatg acatgcgcca gggattgcgc aaaccataat gccagatctc
     4261 attcccaaat ggattgtcgg cagggatggt atacgaatca ccggcatcta catcgattct
     4321 caatattttt cccagcaggg tttccagcga ttgggcattg ccggccgggt cgcctcctgc
     4381 gccgccatcc ccgagaccca tataaaggta accatccggc ccaaatacca tcgcaccacc
     4441 gttgtggttc ggaaaaggct gatctacggt tagtaggatt gcttccgagt ttgggtctac
     4501 tgtggcacct ccatttgact gaaagcgaga tatgaatgtg ttgccacctc gcccggtgta
     4561 attgacataa aagaatccat tttccgaata attcggatgg aacgccagac ccaataaccc
     4621 catttcgtta ctttggtcat tcacgcggtc ttcgatattc aggaagggag tttcactggg
     4681 gtttccatct tctgcatcct gaaataaatg aacatgtccc aatttctcca cgagatacag
     4741 atttccactt ccatccggct gcaagtcaac cggtcggtct agtcccgatg ccaccggttg
     4801 ccatacatag gcattgggat ccgggaaagc tgtcactccc gggagggtgg gaggctgttg
     4861 cgttggcgcg atctcagaag gggcagagat cgtagaaatt gcggttgggg aagcagatgt
     4921 ttctgttact ggttcaagtg tcggtgtaga agggggagtt gtgcctgttc cagtgcatgc
     4981 aagcagtgga agcatcaaga aaaggattaa tcgcctcata agagattact gattatccac
     5041 atcggtcgtt tcggcatcca cgacctcacc accttcgctc aaggccaggt cctgcatgtg
     5101 ctcattgacc acattcgtcg gacaaagctc tacgaagagc gttgagccga tccagaggat
     5161 cgcggcgtca tccagagcac cgataatcgg taacgcgatc gcgtcgatcg gccagaagag
     5221 gtacgccagc gaggcaagcg gtaaaaactt aacaaaaaaa cttacgcggc ggtcgcccat
     5281 caaacgcgta atcaatttaa gacgcatcac catatcacgc atcatgccac cttgcggcac
     5341 tacaattttc tttttctcag ccatgtcaat ctccttttca aaatctggaa cagatcattt
     5401 tttatttgtt ggttataaca cattcaaatg tatcaatacg gtatacgaaa tatatctcat
     5461 aaagtagcga tataacatcc ttcattagtt ttttctcatt gatatgttgg gtgaatatgt
     5521 cgataaaaat aatatgataa gcttgtgcgg tagtcattat ttattaccga tttcggagga
     5581 aaagcgttat gcaacgaaga agaatgtcaa tcgccctgtt ctgcctgggc gtgtttatta
     5641 tggcttgtac gtttggcgga ctcgttccct cgctctccaa cgaaacaccc accagtgcgc
     5701 caaatgtgac ggatctacct gccactcaaa ccgtatcgca gactggcgat atggatgcta
     5761 ccctggatcg gctgggcggc cagccctgtg tcgaaaaacc ggagttcacc tgtgtgacca
     5821 ttcaggttcc gttgaaccac tttgactcct ccaatacgga aaccctggat attgtctttg
     5881 ccgtcctgcc ggcttccggc gaacggattg gcatgtatgt gcaggccttt ccgggaggtc
     5941 ccggtgggga ggggatcagc aatgcgacca ctgtatttta tcccgatgaa attttggaac
     6001 acttcgatct tgtctttttc gatcagcgag ggatcggtct ttcgaatccc ctggcttgcc
     6061 cgaatgcgta tgccgatgat tttcttggat acttgaatgc cacagaccag gcaggcagcg
     6121 aagggttaga cacgcctgag gaacagcagg cagccattgc ctcagcacgt acgtttgtgg
     6181 aaacttgtgt agctgaaata gggatcgaac ctgccaaact aagtttctat gggaccgatc
     6241 aggttgctga agatcttgaa tcgtttcgcc agacagttgg cgatgaaaag ttctggctgt
     6301 atggtgtcag ttatgggacg gccgtcgctc aaacctatgc agctgcccat cccgaacacc
     6361 tggcgggctt aatcctggat ggcacaattg atatgacgtt aaacggcgaa gaaggcgcgc
     6421 ttgcgcagga aaaggcgttt gacaaagtgc tgcttgccgt cctggaagct tgtgatcagg
     6481 atgaagcttg tgcagcggaa ctgggtgggg atgcgttatc agtctacgac aaccttgcag
     6541 cacaggtgtc tgagaatccg atctcctatg aactgccact tccttccggt gaaaagctca
     6601 gcaaaacgtt cacgttcaat caattggagt ttactacggc gtatcagatg tattccctgg
     6661 gtgggcgaat gctgtttctg cgcgcgctgg ctgccgcgca agatggggat atggtcccgc
     6721 tggcccgcct gttacatcaa caggcttcgc tcgacccggc gactggtgca tatcttggtg
     6781 accccacttt ttcagatacg atgttctata gcgtcaactg caccgatgac tcctattaca
     6841 ctggcacgcc agacgagcga attgcgcagg tcatcgagga aggtcagaat tccaatggga
     6901 cggtgccgcg cctcgatgga agtgtttaca ctggcttgtc ttgcgcgttt tggcccagtg
     6961 caccacaaga tgcagtggac cgtgagccat tgatcgctga aggagttcca accattgttc
     7021 tgaatgccac gcttgacccc gccaccccat tcgagcaggg gaaaaccgtt ttcgaacggc
     7081 tggataatgg ctatcacctt tatgtagagg gtggactcca ttccatcttc ggttatggcg
     7141 agacgtgccc cgatgattac gttactgatt tcatggttcg cgggcaactt ccatcggagc
     7201 gcgaaatcgt ttgttcctgg gatccggccg tcataaacgc ttatgttcct ttgatgccca
     7261 aagacgtaag tgcatttgct gatccgctcg aaatcttttc agcgattgat tcagaacttt
     7321 ctcttcagcc tgagtattac tatagttact tcaccgagga cactacattt gcttgcccgt
     7381 acggcggtgc gttcaccttc ggtccgagtg atgcaggcga atcatataat ttcactgatt
     7441 gtgtatttac caatggcttc atcatgacgg gtaccggcag ctttgattat tcgagtggta
     7501 ttttcagact cgaaacacaa gtcagcggtg aaaagaccgg cacattaacc tatactcacg
     7561 attacaggac cggcgaggtt tctgttaccg gagactacgg cggagagacc attgatttac
     7621 agcaataggt atggtgataa aaacccatgc cttatcttgt tgatggtcat aatctgattc
     7681 cgaagcttgg gttgcgattg gattccatgg atgatgaaat ggagttgatt gccatccttc
     7741 aggagttttg cagagtcgaa cgcaaacaaa tagtagtcta cttcgatggt gcgcctcaag
     7801 ccgggacccg caagctgggt cccgttaccg cgcattttgt gaaactcgga acgactgccg
     7861 atgactccat ccgcaggcat ttgaagaaac ttggcaaggc cgcgaaaaat tggaccgttg
     7921 tttcatctga tagacaggtg caggctgaaa cccgcgcggt ccgtgcagag atcatctcat
     7981 ccgatggatt tgcaaagcaa ctgagacaag cacgccacgc ggctccaaag tcggatgaag
     8041 ctcgcacgtt gtctcctgat gaaattgaag aatggttgaa actttttaag cgcaatggat
     8101 aggggtatat aatctgaagc atgagcacta tatattcgta tgttccctct cccaaccata
     8161 tctaccctga tcatccagag cgaccggcac gcctcgatat tctagagcca cgcctgaaat
     8221 cattcgctgc agaatcgata gaagcgaaac cagcagcgca tcaggaagtc gaatatgtcc
     8281 atcatccaaa actggtcagc gggctggaaa aggtatgtct ggaggacgcg ccggggatca
     8341 tagattatgc tcccacctac gtaacaccct cctcgtttca tgatgctctg ctggcggtgg
     8401 gcggagtcat cgcttgcacc cgagccgtca tgaacggtga tgcgaagaac gccttcgcga
     8461 tcgtgcgccc gccggggcat catgcagaac ctgaccgtgc catggggttt tgtctgttca
     8521 ataatgttgc catcggtgcc cagtacgcgc ttgcccatgg attagaacgc gttgcggtga
     8581 tcgattacga cgcgcatcat ggcaa
//
LOCUS       DASTJP010000040         8198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1153958, whole genome shotgun sequence.
ACCESSION   DASTJP010000040 DASTJP010000000
VERSION     DASTJP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153958"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFQ23_01215"
     CDS             <1..563
                     /locus_tag="VFQ23_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET9905218.1"
                     /translation="TGGEVAISTGFGIGAPVVASLADQFVALGVQQFAIVGMAGGLQP
                     ELNSGSLVVCNKAIRGEGVSQHYLPPGQTVDGSEEMANGLSHVLENRGQPHTCGTVWT
                     TDAPFRELRKNVLEYQRAGVLAVDMETAALFSVARSVSRSAVAALSIADQLSDGQWRM
                     TKDLRLTQKGLQLLFDSLMEFLSIKT"
     gene            complement(610..783)
                     /locus_tag="VFQ23_01220"
     CDS             complement(610..783)
                     /locus_tag="VFQ23_01220"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HET9905219.1"
                     /translation="MNGTLRDPVCGMDVTYDSAQARSDYNGQTYYFCALDCKEAFDRE
                     PEKYVYHEEELPR"
     gene            complement(956..2356)
                     /locus_tag="VFQ23_01225"
     CDS             complement(956..2356)
                     /locus_tag="VFQ23_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HET9905220.1"
                     /translation="MKNSNVRGYLPNDAPPMAQRILLGFQHVLTMFPATVLVAAICGF
                     HIGTVLTISGVGTIVALLLAKWRIGKFIPLFYGSSFSYIAAYLAIAQQMTGSVPQFGV
                     PLPNEVISTMQAGIVVTGLLNIIIGFVIRSVGKERVDMVLPPIVTGSVAAIIGFGLAF
                     AALGQMANANFLVAFVTLLATILFSVYLQNKGFLGMIPVLLGALVGYVFSMIVAPNPD
                     QFAPLQNAAWFALPNITLPNFSGPFVGTAIFSIAIMAIATIPESTAHLYQISLYVDRV
                     AEDMGREKYELDKNIGFNLILDGIDDTLKGLFGSTAGTNYGENNSLMAITRNYSGPAL
                     ITAGIIAVLLGFIGKLAGLVQTVPLAVSGGLAIYLFGVIGMQGIALMQEHKVSMFDPR
                     NLAIGAVIMIVGIGGNIGFPGGFIPITIPGIFPSGLPAIATAAVLGILLNVIFLIFKP
                     EGEAERAPSAMAAADD"
     gene            complement(2718..3926)
                     /locus_tag="VFQ23_01230"
     CDS             complement(2718..3926)
                     /locus_tag="VFQ23_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905221.1"
                     /translation="MFGIPYNLFMPFFSVYMLALGVTDKQIGSIASLGLILQIFTALL
                     SGAIVDKFGRRLTLFICDVLCWSVPCLIWAVAQDVRYFAAAALMNSFWRISHTAWTCL
                     MVEDAEERHLVHIWTWIMIFAVCSAFFAPLGGWFVERVGLVPAMRALFLFGFVVLTAK
                     FGLLYIYSHETVRGVQRMQETSHLSITELLREYRGVFKQLFYSRPLLAALSLMVITSI
                     YTTISNNFWGVLFTGKLGFPDSEISTYVAIRSIVMTICFFLIGPRLTNLRRFRLPLWV
                     GFFAFFVSQLLLVVMPPQAVYLLIISVVLEAGAASLVSPMTESLLALSLESAERARVS
                     AMVYVALIVLISPFGWIAGQLSSIDRSLPFVLNTILFAIGIILVWLISHPRFMLTHTE
                     GEKATGSFTP"
     gene            4223..4693
                     /locus_tag="VFQ23_01235"
     CDS             4223..4693
                     /locus_tag="VFQ23_01235"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HET9905222.1"
                     /translation="MRLQGEVTIRAPRKKVWDFMTDPNQIGQCAPGVEKIEMIEPLKR
                     YKGIVSVGFGAVKARFSGDVEVTELQEPNYAKLKAHGSASGSVADATSEMRLSDGADG
                     ATVLSWTADVVVSGQLASLASRLMLPVSQKLAQVFYEQVRKKIETENTTASSTD"
     gene            4743..5825
                     /locus_tag="VFQ23_01240"
     CDS             4743..5825
                     /locus_tag="VFQ23_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET9905223.1"
                     /translation="MMKFEPVPLTEAKGKILGHNIAGANGQRLLRKGRPLTDDDLEKL
                     HALGRNSVYVAELELDDVEENKAARRIAEAICGTGLHISGGASGRANLLTDKMGMLRI
                     DVDRLARINEHHGITLATLVTNSPVHARQIVATVKIIPYAVPESIVSQVEAIANESRH
                     IVRLDLLPSHSVGMILSGSTSIHQKLVSDFVPLRERVERLGSSLTRTDFVALDDETDE
                     TKLAEMLKQQVSAGMKLILIAGETAIMDTHDIVPRAVERAGGHVESVGAPVDPGNLLM
                     LAYLNDVPIVGAPGCARSKKINIVDWLLPRLLVGDRITRRDIVELGHGGLLQDVRERG
                     MPREIKEEDPEPENEPESITVASESP"
     gene            5891..6373
                     /locus_tag="VFQ23_01245"
     CDS             5891..6373
                     /locus_tag="VFQ23_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9905224.1"
                     /translation="MAQTVSITVNGMACVRDVEPRLLLTHFLRDVIGLTGTNIGCDTS
                     QCGACTVLMDGVAVKSCTVLAVQADGSSVTTIEGLVSDGKLHPVQNAFWEKHGLQCGF
                     CTPGMIITVVDLLQRNPHPTEDEIRHGLDGNYCRCTGYQKIVEATQYAAEAMSASEPA
                     "
     gene            6386..>8198
                     /locus_tag="VFQ23_01250"
     CDS             6386..>8198
                     /locus_tag="VFQ23_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET9905225.1"
                     /translation="MATQTLGASIKRKEDPRLLTGEAKYLDDVQLPGMLYAAILRSPY
                     AHAKIKSINKDKAIKVPGVVTILTGEDFKDLPPLPCAWQAEAGRIQNNVNTPRVLEID
                     RVTHTGMGVAVVVAENRYIAEDALNLIEVDYEPLPVVVDAEKTTQAGAPQLHENAPNN
                     ICFDWSCGNKDGTDAALGSAEVVVKQRLVNQRLIPNSMEPRGCIAQYMPASEEYTVWM
                     TSQDPHIMRLLMTAFVFGIPETKIRVIAPQVGGGFGTKIFLYPEYCLMVALSKKIGRP
                     VKWMETRRENYIATTHGRDHVTDIEVGANNDGTITALKVKTFANLGGVLSTIAPGVPT
                     TLYGRVLSGAYKIPHIYCNVLGTYTNTAMVDAYRGAGRPEATYVVERAVDLVARELNM
                     DPTEVRRKNFIQPEDFPYVPADNLLAGLKYDSGDYEKALNTALEMVNYSSFRKEQEEA
                     RKQGKYLGIGFSTYIEICGAAPSSWIGVGPTGQGWGAAMWESANVRVHLTGKVIVTIG
                     TQSHGQGHETTMAQIVAEELGIPMEDITVQLGDTLGTPFGYGTYASRSAAVGGTAVLK
                     SAQKIKEKAKRLAAHMLEAAPEDMTFEAGKAFVMGSPD"
BASE COUNT         2186 a         1978 c         2122 g         1912 t
ORIGIN      
        1 atacgggtgg agaggttgcg atttccacag gttttggaat cggtgcaccc gttgtggcca
       61 gcctcgccga tcaatttgtg gctttgggtg tacagcaatt tgccatagtt ggtatggcgg
      121 gtggattaca gccggagctg aactcgggaa gcctggtcgt ttgcaataag gctatccgcg
      181 gagaaggggt ctctcaacat tatttgcctc cgggtcaaac cgtcgatggt tctgaggaaa
      241 tggcaaacgg gttgagtcat gtgctcgaga atcgcgggca acctcacacc tgcgggacgg
      301 tttggacaac cgacgcgccg ttccgcgaat tgcgcaaaaa cgtcctggag tatcaacgag
      361 ctggagtgct tgcagtggat atggaaacag cggcgctatt ttcggtagcg cggtcagtga
      421 gtcgatcggc tgtagcagct ctttcgattg cagatcagct atctgatggt caatggagaa
      481 tgacgaaaga tttacgtctc acgcaaaaag ggctgcagtt gctttttgat tcgttgatgg
      541 aatttttatc cataaaaacg taaatgacct gctccaccga gcaggtcatt ggcacgcagg
      601 aaagtcccgt cagcgaggga gttcttcctc atgataaaca tacttttctg gctcccgatc
      661 aaatgcctct ttacagtcca atgcacaaaa ataataggtt tgaccgttgt aatctgaccg
      721 ggcttgagcc gagtcgtacg tcacatccat cccgcaaacg ggatccctca atgttccatt
      781 catcttgact tctccttcct gttgaaagca ggtttgtagt tggattatgc tcagggtatg
      841 aaataccgtc aatacgggct tcgcctttgc ccatagggat ttccctaact taaaacaaca
      901 acgggaggac tgacgcatca gtcctcccgt tacttcggta atgataaacc ttgaactaat
      961 cgtccgcagc ggccatggcg cttggggctc gctcggcttc gccctcaggt ttgaagatca
     1021 ggaagatcac atttaacaaa atgcccaaaa ctgctgctgt tgcgatcgca ggcaagccac
     1081 tcgggaaaat acccggaatc gtgatcggaa tgaatccgcc gggaaagcca atgttgcctc
     1141 caatgccgac gatcataata accgctccaa tagccaggtt gcgcgggtca aacatgctga
     1201 ccttatgttc ctgcatcagg gctataccct gcataccaat aacaccaaac aggtaaatgg
     1261 cgagtccgcc gctcactgca agtggaactg tttgaacaag ccctgccagt tttccgataa
     1321 atccaagcag caccgcaata ataccggctg tgatgagcgc cggaccagaa tagtttctgg
     1381 taatggccat cagcgagtta ttttcgccgt agtttgttcc agcagtcgag ccaaataatc
     1441 ccttcagagt gtcgtcgatg ccatcgagaa tcaagttaaa gccgatgttt ttatccaact
     1501 catatttttc gcggcccata tcttctgcca cgcgatccac atagaggctg atctgataga
     1561 ggtgcgcagt ggactcaggg atggttgcaa tcgccataat cgcgatgctg aaaatcgctg
     1621 tgccaacgaa cggaccacta aaatttggta gtgtgatatt cgggagtgca aaccaggcgg
     1681 cattttggag cggtgcaaac tgatcagggt ttggcgccac aatcatcgaa aagacataac
     1741 cgaccagcgc tccgagtagg acggggatca ttccgagaaa gcctttgttt tgcagataca
     1801 cggagaatag aatggttgcc aataaggtaa cgaacgcgac taggaagtta gcgtttgcca
     1861 tttgccctaa agccgcaaat gccaatccaa agccgataat cgctgctact gagcctgtca
     1921 cgattggagg cagaaccata tcaactcgtt cttttccaac gctacggatt acaaatccta
     1981 taatgatgtt caacaaacca gtcacaacaa tgccggcctg catggtgcta attacctcgt
     2041 ttggaagagg tacgccaaac tggggtacac tgccggtcat ttgctgagca atcgccagat
     2101 aggccgcgat ataactgaaa ctcgacccgt aaaatagcgg gataaatttg ccaatcctcc
     2161 acttcgcaag aaggagtgcg acaatcgtgc caacaccgga aatggttaac acggttccaa
     2221 tgtgaaaacc acaaatggcc gcaaccaata ctgttgcagg gaacatggtt agcacgtgct
     2281 gaaaacctaa aagaatccgc tgcgccattg ggggtgcatc attgggcaga tagcccctta
     2341 cattactatt cttcataatc gtttctcctc gatttgaacc agctgaattt gacttgacgg
     2401 ttttcgtcct tgctgatttt gcttttcctt tttgattttt tgccattgtt cttgctcctc
     2461 ctgtaaatta cctgatcggt agctacaatc gtttcgacgc aaaatccatc ttcacctcca
     2521 aataaatcgc catacataat tcatgtatgg cgaaatcaaa ataagggccc atcggcagca
     2581 ctattgcaaa tgcgtaggat ggaaaataca ctcacagaac ggatttgcag cgttctcatc
     2641 ttattttaat taaccctgat ccccgaaagt ggatacgccg ccttgcggcg gttttttatc
     2701 tcctgctcga ctgcaactta cggtgtgaag gaccctgttg ccttttcgcc ttcagtatga
     2761 gtcagcatga atctcggatg gctgattaac cagaccagaa taataccaat tgcgaatagg
     2821 atggtattca gtacaaaagg tagagaacgg tcgatcgagg aaagttgacc tgcaatccag
     2881 ccaaagggcg agatcagcac aataagagca acgtagacca ttgcgctgac gcgtgcccgc
     2941 tctgcagatt ccagcgatag tgccagcagc gattcagtca tcgggctgac gagagaagct
     3001 gcccctgcct ccagtacaac gctgatgatc agcaaataga ccgcctgggg aggcatgacg
     3061 acgagcagta attggctcac gaagaacgcg aaaaatccca cccataatgg cagccggaaa
     3121 cgacgcagat ttgtgagacg cggcccaatc aggaagaaac aaatcgtcat gactatcgaa
     3181 cggatcgcga cgtaggttga aatttctgag tcaggaaagc caagtttccc cgtgaacaag
     3241 acgccccaaa agttgttgct aatggtcgtg taaatgctcg tgatcaccat cagggataga
     3301 gccgccagca gtggtcttga gtagaagagt tgtttgaaaa cgcctcgata ttctcttaat
     3361 agctccgtga ttgaaagatg gctggtttcc tgcatgcgtt gcacccctcg aaccgtttca
     3421 tgagaataga tatataacaa tccgaatttg gctgtcagca caacaaaccc aaagaggaat
     3481 aaggcacgca tggctggcac taaaccaacg cgctctacaa accacccacc cagcggagca
     3541 aagaaggccg aacaaacggc aaagatcatg atccaggtcc aaatatggac gaggtgtcgc
     3601 tcctcagcat cctcaaccat gagacaagtc caggcagtgt gcgagatgcg ccaaaagctg
     3661 ttcatcaaag ctgccgcagc gaaatagcga acatcctgcg caacagccca gattaagcat
     3721 ggaacactcc aacatagcac atcacaaata aacaaggtta agcgccggcc gaatttatcc
     3781 acgatggcgc cactcagcaa agccgtgaat atctgcagaa taagccccag gcttgcgata
     3841 ctcccgattt gtttgtccgt gactcccaga gctaacatat aaacagagaa gaatggcata
     3901 aacagattat agggaatgcc gaacatgatc tcggtcaaca ccgtgatacg cggatttcct
     3961 ttcaattcgc gtagcgtttg aaataaagga tgatttagaa acaagggttt atgttccctt
     4021 ccagagtttt catgggtgtt taatggaatg ggattttacc caattaatct gtaattcatg
     4081 tcccgccgtt gacattctta ctgtcattgt gctacactcg aattgcccaa aaaaattgca
     4141 accgtatctt tcacagtttg ttccaagacc tcacaagcaa agtttgactc acatcatcct
     4201 taaaacctga aaaggaacgc agatgagact acaaggtgaa gtcacaattc gtgcaccgcg
     4261 gaaaaaagtt tgggacttta tgactgatcc gaatcagatc ggtcagtgcg cgccgggtgt
     4321 cgaaaaaatt gaaatgattg aaccattgaa gcgttataaa ggtattgttt cagtgggttt
     4381 tggagcagtc aaggcgcgct tttcggggga tgttgaagtt actgaactac aagaacccaa
     4441 ttatgcaaag ctaaaagccc acggttctgc cagtggcagt gtggccgatg cgaccagcga
     4501 aatgaggctc agcgacggtg cagatggcgc gaccgttctg agctggacag ccgatgttgt
     4561 tgtctcaggt caattggcaa gcctggcatc cagactgatg cttcctgttt ctcagaagct
     4621 tgcacaagtg ttttatgaac aggtgcgcaa gaaaattgaa acggagaata caaccgcgtc
     4681 atcgacagat tagatttatc aatttggttt gctcacaatt catactgtcc acttctggag
     4741 aaatgatgaa gtttgagccg gtgcctctca ctgaagcgaa gggaaaaatc cttggtcaca
     4801 acatcgcggg ggcaaatggt caacgtttgt tacgcaaggg aagaccactt acggatgatg
     4861 acctggaaaa actgcatgca ctggggcgaa actccgtcta tgttgctgaa ctggagctgg
     4921 acgatgtaga ggaaaacaaa gctgcgcgtc gtattgcaga agcgatttgt ggaacgggcc
     4981 tacacatttc tggtggagca tcaggccgcg cgaacctgct aacagacaaa atggggatgc
     5041 tacgaatcga tgtggatcga ttagctcgaa tcaacgaaca tcatgggatc acgctggcaa
     5101 cgctagtgac gaactcccct gttcatgcga gacagatcgt tgcaacggtc aaaataatcc
     5161 cttatgcggt tccggaatca atcgtcagcc aggtggaagc aattgcaaat gagagcaggc
     5221 atattgtccg cctcgatttg cttccttctc attcggtggg aatgatctta tctggctcta
     5281 cttcgataca ccagaaatta gtttcagatt tcgttccact ccgcgagcgt gttgaacgat
     5341 tgggttcttc actcacgcgt acagacttcg tcgcgctgga cgatgaaacg gatgaaacaa
     5401 aactggctga gatgttgaaa caacaggttt cagccggtat gaagctgatc ttgatcgccg
     5461 gtgaaacggc cattatggat actcatgata tcgttccgcg ggcagtggaa cgtgcgggtg
     5521 gacatgttga atcagtgggc gcgccggttg acccgggcaa cttgttgatg ttggcctacc
     5581 taaatgatgt gcccattgtt ggggcccccg gttgtgcgcg cagcaaaaag ataaatattg
     5641 tggactggct tctgcccaga cttttggtgg gagaccgaat cacacgcagg gatatcgtcg
     5701 aacttggaca tggcggcctt ttgcaggatg tacgcgaacg cgggatgccg cgcgagataa
     5761 aggaggaaga tcctgagcct gaaaacgaac ccgaatcaat taccgtagct tccgaaagtc
     5821 catgactccg gttcataagg tgctggagtt ttcttttttt gaaattcaaa ttgagaagga
     5881 ggatcaggtt atggcgcaaa cagtttcgat cactgtcaat ggcatggcgt gtgtgcggga
     5941 cgtggagccg cgcctattac tcacgcactt cctgcgtgat gtgattgggt tgacgggaac
     6001 aaacattgga tgcgacacca gtcagtgcgg cgcgtgtacc gtgctcatgg atggcgtcgc
     6061 ggtgaaatcc tgcacggttc tcgcagtaca ggcagatggg agcagtgtca ccaccattga
     6121 aggacttgtc tctgatggaa agttgcaccc ggtgcagaat gccttctggg aaaagcacgg
     6181 acttcagtgc gggttctgta cgccaggcat gatcatcaca gtcgtggacc tgcttcagcg
     6241 aaacccgcat ccaaccgaag atgagatccg ccatggcttg gatggcaatt actgccggtg
     6301 cactggctac cagaagatcg tcgaagcgac ccagtatgcc gctgaagcaa tgtcggcttc
     6361 tgagccggct taacaggagg tcacaatggc aactcaaacc ttaggtgctt ccattaaacg
     6421 caaagaggac cctcgtctgc tcacgggcga ggccaaatat ctggatgatg tccagcttcc
     6481 aggtatgctg tatgccgcca tactgcgaag cccatatgct catgcaaaaa tcaagagcat
     6541 taacaaagac aaagccataa aggtgcctgg tgtggtgact atcctcactg gcgaggattt
     6601 taaagatctg cctcctctgc cgtgcgcctg gcaggctgaa gctggccgca ttcagaacaa
     6661 tgtcaacaca ccgcgcgtgc tggagattga tcgcgtcacg catacaggga tgggtgtcgc
     6721 agtcgttgtt gcagagaatc gctatattgc cgaagatgct ctcaacctga tcgaagtgga
     6781 ttatgagcca ttgccggttg tggtggatgc agagaaaacg actcaggcgg gcgctcctca
     6841 actccatgag aatgcaccta acaacatttg ttttgattgg tcgtgcggca ataaggatgg
     6901 cacggatgcc gcgcttggct ctgcagaagt cgtggtcaag cagcgcctgg tcaatcaaag
     6961 attgattcct aactccatgg agccgcgcgg ctgtattgca caatatatgc cagcatcgga
     7021 agaatatacc gtgtggatga cgtcacaaga cccgcacatc atgcgccttt tgatgacagc
     7081 ttttgtgttc ggtattcccg agacgaagat ccgcgtcatt gccccgcaag tcggcggcgg
     7141 atttgggaca aagattttcc tctacccgga atattgtctt atggtggcgc tgtccaagaa
     7201 gattggacgg ccggtcaaat ggatggagac ccggcgtgaa aattacatcg ccactacaca
     7261 tggacgcgat catgtaacag atatcgaggt cggagcgaat aatgatggaa ccatcaccgc
     7321 gctcaaggtc aagacctttg ctaatctcgg tggtgtccta tcgacgattg cgcccggtgt
     7381 tcccacaacc ctttatggtc gtgtgctctc tggtgcctat aaaattccac acatctattg
     7441 caacgtgctt gggacgtata cgaatacagc catggtagat gcgtatcgag gcgcgggacg
     7501 accagaagca acctacgtcg ttgagcgtgc ggttgacctt gttgcgcgtg aactaaatat
     7561 ggatcccaca gaagtgcgcc gcaaaaattt catccagccc gaagacttcc cttatgtgcc
     7621 cgcagataac ctgctggctg ggctgaaata tgatagcggc gactacgaga aagcgctaaa
     7681 tacggccctt gagatggtga actattcctc attccgcaaa gagcaggagg aagctcgcaa
     7741 acagggcaaa tatttaggaa taggtttttc cacgtacatc gaaatttgcg gcgccgcgcc
     7801 ttcttcatgg attggagtcg gccctaccgg tcagggatgg ggtgccgcga tgtgggagag
     7861 tgcgaacgtc cgcgtgcatc tgactggtaa agtcattgtc accatcggaa cccagtcaca
     7921 tgggcaaggg catgaaacga cgatggcgca gatcgtcgcg gaagagttgg gaataccgat
     7981 ggaggatata accgttcaac ttggtgatac gcttgggaca ccatttggct atggcacgta
     8041 tgccagccga agcgcggcag tcggaggcac ggcagtgctc aagagcgcgc agaagatcaa
     8101 ggagaaagca aagcgactcg ctgcacatat gcttgaagcc gcccctgaag atatgacgtt
     8161 tgaagccggc aaagcatttg tgatgggctc accagata
//
LOCUS       DASTJP010000041         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1160386, whole genome shotgun sequence.
ACCESSION   DASTJP010000041 DASTJP010000000
VERSION     DASTJP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160386"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFQ23_01255"
     CDS             complement(<1..725)
                     /locus_tag="VFQ23_01255"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET9905226.1"
                     /translation="MLSFLSWYLLISLLGWATFPLTYHLFSALSDRGYSLSRAFGLLV
                     WGYVFWLFASLGIAQNDVGGLLLGLVILGGLSAWIFSNQRAQIIGWLRENRRLVLTVE
                     ILFLVAFSLMAFFRSANPEIVGTEKPMELMFINGIMNSPTFPPRDLWLSGYSISYYHF
                     GYVMTSMLALFTGVPATMAFNLMIALIFGLSALVAYGILYNLLANYHASTLDAHDLLN
                     ESSAGAEHQNSSILRPLFGPLFLL"
     gene            complement(766..1122)
                     /locus_tag="VFQ23_01260"
     CDS             complement(766..1122)
                     /locus_tag="VFQ23_01260"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HET9905227.1"
                     /translation="MIFHITSRTNWETAQQRGDYRAESLETEGFIHCSTISQVLPVAE
                     NYYKGQSGLILLGIEPALLSSDLKWEPPSGGAPPPGVPEGDPFPHIYGPINLDAVIKV
                     LELRLDSVDKFVLPEL"
     gene            complement(1119..>2957)
                     /locus_tag="VFQ23_01265"
     CDS             complement(1119..>2957)
                     /locus_tag="VFQ23_01265"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET9905228.1"
                     /translation="AFSIGWNLLADHRPRTEDEGQSSMVQSPFVAGLSASFLTILLGN
                     LGTIQLLFQRLQQLGALDQFNNTDATIIQRWKWAFDGFLLTLKGSSLPIGFGDWYWNP
                     SRVIPPGPGNEITEFPLFTFLYSDLHAHMIAMPLALLALSWALAILAGRAKWRNALAA
                     GAAFAIGGLIIGALYPTNLSDIYTFLPIGLAAVGYTIWRYTDDVSTPKRIALSLAGLV
                     ALTVLSFVMYQPYRAWYSQAYSALDPWRGPYTPIWSYLTHWSVFLFILASWMAWETRE
                     WMAETPVSALRKLKPYQPIIWGALIIFVISLFALQFQHFGEEVQHTGTSVGWIALPLA
                     IWAGILLLRPNLPDAKRFVLFLIGTALLITIVVDVVVVRGDIGRANTIFKFYLQAWLL
                     LAVSAGAAFAWTLPAFFRWLPGWRVFWQTAVILLVAGAALFTASGTAGKIRDRWIVEA
                     PRTIDSITFMNYAHYDDFGQRLDLSEDYRAIRWMQENIQGSPVIVEANCPEYRWCTRY
                     TIYTGLPGVVGWNFHQRQQRAFTATWVESRVAEVTNFYGTLDVETAREFLEKYDVSYI
                     IVGQLERAEYKSEDTVNGISKFDEFDGTYWNAIYRDGSTVIYEVMP"
BASE COUNT          842 a          741 c          753 g          621 t
ORIGIN      
        1 agcaaaaaca gagggccaaa gagcggacgt aggatcgatg agttttgatg ttcagcaccc
       61 gcactcgatt cgttaagcaa atcgtgcgcg tcaagcgttg acgcgtgata attagcaaga
      121 agattataga gaatcccata cgcaacaagg gcgctcaagc caaagatcaa tgcgatcatc
      181 aggttgaacg ccatggtggc cggcacgcct gtgaacaagg ccagcatgga tgtcattaca
      241 tatccaaagt gataatagga aattgaatag cctgacaacc acaaatcgcg tggaggaaac
      301 gtaggtgagt tcatgatgcc gttaatgaac atcaactcca tgggcttttc cgtaccgaca
      361 atttcggggt ttgccgaacg gaaaaatgcc ataagcgaaa atgcaaccag aaagagaatt
      421 tcaaccgtca atacgagacg gcgattctcg cgaagccaac cgataatttg agctctttgg
      481 tttgaaaata tccaggcact taatccaccc aggataacta accccagaag caatccgcct
      541 acatcatttt gcgcgatacc cagagaggca aacagccaga atacatatcc ccaaaccaat
      601 aagccgaagg cgcgtgaaag ggagtagccg cgatcactca aggcggagaa aagatgatac
      661 gttaacggaa aagtggccca gccaaggagt gaaatgagga gataccagga aaggaaggaa
      721 agcatagggt ttcttaactt aggtgttgat gcttttcttg aagacctaaa gttcaggcag
      781 gacgaattta tccactgagt ctaacctcag ctcaaggact ttgattactg catcgagatt
      841 gatgggacca taaatatgcg ggaatggatc tccctcggga acgcccggag gtggagcccc
      901 acctgaaggc ggctcccact tcaagtctga ggaaagaagt gcaggttcga tgccaagaag
      961 gatgagtccg ctctgtcctt tatagtaatt ctcggctaca ggcagcacct gtgaaatcgt
     1021 cgaacagtga atgaatcctt cggtctcaag gctttcggcg cgatagtcac cgcgttgctg
     1081 tgcagtttcc caattggttc gtgacgttat gtggaagatc aaggcatcac ctcgtagatg
     1141 accgtacttc catcgcgata gatggcgttc cagtacgttc catcgaattc atcgaatttg
     1201 gaaatcccat tgacggtgtc ttccgatttg tactctgctc gttccaactg cccgacaata
     1261 atataactca catcatattt ttcaaggaac tcacgtgcag tttccacgtc gagcgtacca
     1321 taaaaattgg tgacttcagc gactcgagat tctacccagg tagctgtaaa cgcacgctgc
     1381 tggcgctgat ggaagttcca acccaccacg ccgggcaggc cggtatagat tgtgtaacgc
     1441 gtgcaccaac gatactcggg gcagttggct tccacaatca caggagaacc ctgaatattt
     1501 tcctgcatcc atcgaatggc gcggtaatct tcgctcaaat cgaggcgttg cccaaagtcg
     1561 tcatagtgtg catagttcat gaacgtgatc gagtcaatcg tacgaggggc ttccacgatc
     1621 cagcggtcgc ggattttgcc agccgtgccg cttgccgtaa ataacgctgc acccgcaaca
     1681 agcaggatca ccgccgtctg ccagaaaacg cgccagcccg gcagccatcg gaagaaggcc
     1741 ggcagggtcc aggcaaaggc agcacctgcg ctaaccgcca ggagtagcca ggcctgtaaa
     1801 tagaacttga aaatcgtgtt cgctcgaccg atgtcaccgc gcacgacgac tacatccaca
     1861 acaatcgtga tgagcaatgc agtgccgatc aaaaacaaga caaatcgttt tgcatctggc
     1921 aggtttggac gtaacaataa tatgccggcc cagattgcga gaggcagcgc aatccagcca
     1981 acgcttgtgc ctgtgtgctg gacttcttcc ccaaaatgct ggaattgaag cgcaaacagg
     2041 ctgatcacaa atatgatgag tgcgccccag atgatcggct gataaggctt aagttttctc
     2101 aatgctgaaa caggagtttc cgccatccat tcacgcgttt cccaagccat ccaggatgcc
     2161 agaatgaaga ggaaaacact ccagtgcgtg agataggacc aaatgggtgt atatggacct
     2221 cgccacgggt cgagcgcgct atacgcctgg ctataccagg cacgataagg ctgatacatg
     2281 acaaaggaca aaaccgtcaa cgcgaccaag ccagccagcg aaagcgcaat gcgttttgga
     2341 gtggatacat cgtcggtata ccgccaaatt gtatatccta cggctgcaag cccaatgggc
     2401 aggaacgtat agatatctga gagattcgtc gggtagagag cgccgatgat caacccacca
     2461 attgcaaacg ccgcaccggc tgccaaagcg tttctccatt tggcgcggcc agcgagtatc
     2521 gccaatgccc aacttaatgc aagcaatgcc agcggcatgg cgatcatatg tgcgtgcaag
     2581 tcactgtaga ggaaggtaaa gagcggaaat tcggtgatct cgttgcccgg acccggagga
     2641 atcacacggc tgggattcca gtaccaatcg ccaaacccga tggggagaga ggatccttta
     2701 agagttaata agaagccatc gaaggcccat ttccagcgtt ggatgatcgt cgcatctgta
     2761 ttgttgaact gatccagcgc acccagctgc tgtaaacgtt gaaacagaag ttgaatcgtt
     2821 cccagattac caagaaggat cgtgagaaat gaagccgaaa gcccggcaac gaatggactt
     2881 tggaccatag acgattgacc ctcgtcctcg gtccttggtc tatgatctgc gagtaaattc
     2941 catccaattg aaaatgc
//
LOCUS       DASTJP010000042         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_835803, whole genome shotgun sequence.
ACCESSION   DASTJP010000042 DASTJP010000000
VERSION     DASTJP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835803"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..459
                     /locus_tag="VFQ23_01270"
     CDS             103..459
                     /locus_tag="VFQ23_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9905229.1"
                     /translation="MENFALVIEDDEDLSAIFVKALEAAGFKVEAIRDGALARQRLQE
                     VSPRVIVLDMHLPHVDGATLLKEIHANPALANSRIIMATADNVRAELYRDKATIVLVK
                     PISFSQLRDLSARLKL"
     gene            complement(465..914)
                     /locus_tag="VFQ23_01275"
     CDS             complement(465..914)
                     /locus_tag="VFQ23_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905230.1"
                     /translation="MQWTLVHGVLAKGYRVASGPSKDYPYGALDRQRPIFASRGLDLS
                     EYFNGTLNIDIRPHAFELIKAEFTFHHVEWTDLHPPEHFSFSRCKVVFNEVEYQGWVY
                     YPHPETKLRHFQNPSLLEVIAYPIPDLAYGDELDVLLKAEEIVVTKP"
     gene            complement(988..1737)
                     /locus_tag="VFQ23_01280"
     CDS             complement(988..1737)
                     /locus_tag="VFQ23_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905231.1"
                     /translation="MRPQPFLASLLLILSMLACNLPSSAPGTPTATILATNTSSIQST
                     SIPSSTSPPSSTAPATLTSTPSVPSASAKDVAVNCRFGPGTVWVALSALNVGQSAQIV
                     GKNADGSWWYIADPFSSGRSCWVAASVTNASGNLSSIPVVEAPRASVTNVAVRVDPSS
                     IAVAGCVGPAAPIKFIGTIEVNGPARVTWNFDSQQGGAFPAQNTDFDTFGSRDVSMDF
                     TPTLTAGTYWVRLIVTSPNNLQSETRYTIQC"
     gene            1949..3016
                     /locus_tag="VFQ23_01285"
     CDS             1949..3016
                     /locus_tag="VFQ23_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9905232.1"
                     /translation="MAQRITRATIKEVASMAGVSTQTVSRVINERPDVSPETRKRVQE
                     VIKALSYQPSALARSLIRQRSHTLGVVTAGLKHLGPSRTLSGITSAAEEAGYSLLLKE
                     LPTYDTEDVQPIFQAFLSHHVDGIIWAVPEVGENRKWVNNPPADIEIPLVYITMEPRN
                     NLSIVSIDNYLGGRIAMAHLLEQGYHKIGHISGPLDWWEARQRMAAWKDVLTEAGRNA
                     EDSHCVEGNWSSASGALAIEKLFYQYSDMDAIFVANDQMALSVLQFFAEKQIRVPEDM
                     GIVGFDNIAESAFFSPALTTVQQDQHNVSKVAVAEVIKVIEAGWQELDPVAPQSIILP
                     PTLIVRQSSLRIQEGGEVKEN"
BASE COUNT          820 a          751 c          793 g          764 t
ORIGIN      
        1 accttacata gttgtttatt cgtattagcg tgctttcatt ttggataggg taaaatcttg
       61 gatggaaatt cgtagtacaa cagtgaccct aaaggatatg caatggaaaa ttttgcgctt
      121 gtgatcgaag acgatgagga tctttcggcg attttcgtaa aagcactgga agccgctggg
      181 tttaaagtgg aggcaatccg tgatggagca ctcgcacggc agcgtttgca ggaggtatcc
      241 cctcgcgtca tcgttctcga catgcatctc ccgcatgtcg atggggccac cctgctcaaa
      301 gagatccacg caaatccggc gttggccaac tcaaggataa tcatggctac cgcggataat
      361 gtgcgggctg agttatatcg cgataaagcc acgatcgtgt tggtaaaacc catttcattt
      421 tcgcaactgc gagatttaag tgcccgcttg aaactttgaa aggttcaggg cttcgttact
      481 acaatttctt ccgccttcaa aagcacatcc aattcatccc catacgccag atctgggatc
      541 gggtaagcga tcacttcaag caaggacgga ttctgaaagt gacgcagttt tgtttccggg
      601 tgcggataat acacccagcc ttgatattca acttcattaa aaacaacctt acatcgtgaa
      661 aaagaaaaat gctcaggtgg gtgcaagtcg gtccattcca catggtggaa ggtaaattca
      721 gctttgatca attcaaaggc atgggggcga atatcaatgt tcagcgtacc attgaagtat
      781 tcgcttagat ccaatccgcg cgaggcgaag atcggtcgtt gcctgtccag cgcgccgtaa
      841 ggataatctt tcgaaggtcc ggaagcgacg cgatagccct ttgcaagaac accatgaacg
      901 agagtccact gcataagcag attctaactt cttcctaatt aaataagaaa ggcggatgcc
      961 actaacatcc gcctggatgt ccaacgccta acactggatc gtatagcgcg tttcgctttg
     1021 aagattattt ggagaggtaa caatcaacct aacccagtat gtgccagccg ttagcgtcgg
     1081 cgtgaaatcc atcgacacgt cccttgaacc aaaggtatcg aaatcggtgt tctgagcagg
     1141 gaacgcaccg ccttgctgac tgtcgaaatt ccatgtgact cgggcaggtc catttacttc
     1201 gatggtgccg atgaatttaa tgggcgcagc cgggcccaca cacccagcga cggcgatact
     1261 gctcgggtcc actctcacag cgacgtttgt cacagaagct cttggggctt cgacgactgg
     1321 aatcgacgac aggtttccgg aggcattggt gacactcgca gcgacccaac agctcctgcc
     1381 tgagctaaag ggatccgcga tataccacca acttccatct gcgtttttgc ccacgatttg
     1441 cgcactttgc ccaacattca aggcactgag cgctacccag accgtccccg gaccaaaacg
     1501 acaattaact gccacatcct ttgctgacgc gcttggaacc gaaggtgtgg atgttagggt
     1561 tgcaggggcc gtggaagagg gcggtgatgt gctgcttggg atggaggttg actgaataga
     1621 agaagtattt gtcgcaagga ttgtcgccgt tggggttccc ggcgcactcg acggaagatt
     1681 acaggcaagc atgctcagga tcaacaaaag gctggcaagg aaaggctgag gtctcattga
     1741 aatcctttat cttgatagtg aattcgatag cgtatatctt acgctaaagt taaagcaacc
     1801 gcaatagacc gttgggcgct tgatagattc cttattttca tgttgatcaa aacttggttt
     1861 tcgaaactct tgacgcgaat tcctcttttt gctaaaatgt tatcgttccc gttatcgata
     1921 acgggaacga taacgggaaa ccataccaat ggctcaacga attacgcgtg caacgatcaa
     1981 agaagtagcc tccatggcag gagtttcaac gcagactgtt tcacgcgtga tcaatgaaag
     2041 gccagacgtt tcccctgaaa ccagaaaacg tgtacaggaa gtgataaaag ccctgagtta
     2101 tcagcccagc gcccttgccc ggagcttgat acgccaacgg agtcataccc tgggcgtcgt
     2161 tacggcggga ctcaaacacc ttggtccttc tcgtacactg agcggaatta cctcagcggc
     2221 tgaggaggca ggctattcac tcctgcttaa agagcttccc acttatgata cggaagatgt
     2281 tcagccaata tttcaggcgt ttctctccca ccatgtagac gggattattt gggctgtgcc
     2341 cgaggtgggc gaaaaccgaa aatgggtcaa caacccgcct gctgatatcg aaattccttt
     2401 ggtttatatt acgatggagc caagaaataa tctttcaatt gtatccatcg ataattatct
     2461 tggcgggcga atcgcaatgg ctcatttgct cgaacaaggc tatcacaaga ttgggcatat
     2521 ttccggtccg ctggactggt gggaagcacg tcagcgcatg gcggcctgga aagacgtgct
     2581 caccgaagcg ggacgaaatg cagaggatag tcactgtgtg gaagggaatt ggtcctctgc
     2641 cagtggcgct ctcgccattg aaaagttgtt ttatcaatat tcggatatgg atgcgatctt
     2701 cgtcgcgaac gatcaaatgg cgctgagtgt gctgcaattc ttcgccgaaa aacagatccg
     2761 tgtccctgaa gatatgggaa ttgtcggatt cgacaatatt gccgagtctg cctttttctc
     2821 cccagccctt acaactgtcc aacaggatca gcataatgtt tcaaaggtgg cagttgcaga
     2881 ggtgatcaaa gttattgaag caggctggca ggaattggat cccgttgcac cccaatcgat
     2941 tattctgccg ccaacgttga ttgtgcgaca aagctcgtta cgcattcagg aaggaggtga
     3001 ggtcaaagaa aactaaccgc aattcgcgga aagatggagg cacacacgag caggttcatc
     3061 ccagctctgt gaactccccg gaaaggctga ctcatgttgc atagtagtca atttgctctt
     3121 gctaaaca
//
LOCUS       DASTJP010000043         7386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1258106, whole genome shotgun sequence.
ACCESSION   DASTJP010000043 DASTJP010000000
VERSION     DASTJP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258106"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFQ23_01290"
     CDS             <1..262
                     /locus_tag="VFQ23_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905233.1"
                     /translation="KSLKLRLSHFFVQREQILSRDVSPAGRDLVAPEVDGNESIVEDI
                     LRWADDGGQMLPLYIQGNPGTSPESSEYRINKKAEEETVSKQ"
     gene            311..1591
                     /locus_tag="VFQ23_01295"
     CDS             311..1591
                     /locus_tag="VFQ23_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9905234.1"
                     /translation="MAVNPVSQQYSWTFRRVVGATLVLISVILGFWLVYRFNQVIFTL
                     FIAVVIGTVIRPAVAWLHLRGLPPTAGVILVYLFVLLLLIGFLLLLFPLISEQSSTVT
                     SSIPEYYQNLRVWLVNHPNPFLVGLSDFLPAALPTLNLRPVQQTGEELMASAGQVAGY
                     ITSASKVIFTAIILLVLAFYWTLEGPRIIKSFLLVVPQMQRESISDLISAMESKVGLY
                     VVGQGILCMVIGIMALIAYTLIGLPNALVLALIAGVLEAVPMIGPLLGAVPAALVSLS
                     IAPGKLVWVIVATLVIQQLENTLLVPRIMKKAVGVNPFVTLLALFAFSSLFGLAGALM
                     AIPIAAMIQLALNHFVFQQATVEMEVSEGRDHASRLRYEAQDLAQDLRKQARLKKRGS
                     ELEIKQIEHVMDEIETITTDLDALLARTNNSDSK"
     gene            1588..2832
                     /locus_tag="VFQ23_01300"
     CDS             1588..2832
                     /locus_tag="VFQ23_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9905235.1"
                     /translation="MTKQLLVIGTAVMTTLLTLVVLWQFRIVVIYVLISLALAATVRP
                     IASSESRNSAVTRLLTIFLYAVSIGSFGVLIFVVGRLLISDLQELAQILSIQKTWMLP
                     PWLEGSLFQQALTRWLPAPDKIFEAITSQPQLMVSAVLGFTQGVGGLVSGFFVILFLS
                     IYWGINQNHFERLWLSLLPSEQRKHARDIWRTLEHDLGAYTRSEVIQSLLAVLILGLG
                     YWLLGAPYPALLAVVGALAWLVPVVGAALAVILPLLLGLLTGAQFSLFTVLYTLIVLI
                     ALRVWIEPRLFRRTLDNPILTLVILLAMAETFGLLGIIVAPPLSVVCQILWNLLRSDR
                     LAPDPAVQISDLKERQVRLRLALEEIEGPPSPLVVSSMERLNNLLEKASPFLPMAVSN
                     EQSDLFHPSQPLASEDKGANAE"
     gene            3178..3486
                     /locus_tag="VFQ23_01305"
     CDS             3178..3486
                     /locus_tag="VFQ23_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9905236.1"
                     /translation="MPNKRMSKSQFVATLAEKSGLNKKQATSALDTINAMVAQQLGKR
                     GPGEVLIPGLLKLNIVDKPATRKHEGINPFTKEPMTYKAKAAHKVIRVRALKALKDAI
                     "
     gene            3576..4484
                     /locus_tag="VFQ23_01310"
     CDS             3576..4484
                     /locus_tag="VFQ23_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905237.1"
                     /translation="MRRNFRVAAFYGTVSILLILSACITLTPNPSEAEILAAHCEACD
                     QATLAVILTQEKNYADNQAAAAAEIVRANAQSTLNSANAVLSAAQTQDQNNANVLAAQ
                     IAATAEIVRANAQATLDSASSTQNAALTQDVIHQTQMADLATTGAQALVNQQNKDELA
                     ANTIAMQQAQAAATSQWYTDQERQRNVQRQGPITFLWVWCFPVFIVLLAGLVLWGVWQ
                     RLKIQQDNQRILEIPPADKLPASTAEVIHYPRDDSLPYLESDIVGNRYLLTKPDDEVR
                     AWLDEVKNKLRSSDTNKKDEDDYTDN"
     gene            4465..5043
                     /locus_tag="VFQ23_01315"
     CDS             4465..5043
                     /locus_tag="VFQ23_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905238.1"
                     /translation="MITQTIDPQYFEQKALRIRAQVSTILTKWGLLPRFKRWRLTQDA
                     STGMIVLFGILNNRYIATPFSDYFDPRLLHDLANELQLQVVYCNSDGLRYAFILDRGE
                     FGKLPTHVDSLFIEDGQLLAKVVYSDTPQFIPVPPVEVATIDDQTRVQQGVGAFLKVF
                     DDIKLRDDAALELSVQNLPVVVVMDELKSRNE"
     gene            5120..5332
                     /locus_tag="VFQ23_01320"
     CDS             5120..5332
                     /locus_tag="VFQ23_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905239.1"
                     /translation="MKPNNDVLESKWDELRIKLLNWRNKRREDQQIKVNSVREKSINL
                     LQKRYGYSREKAIEELDTRYSGTWLG"
     gene            5370..5972
                     /locus_tag="VFQ23_01325"
     CDS             5370..5972
                     /locus_tag="VFQ23_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905240.1"
                     /translation="MTKTNNLTKWLDIELMNLVRRKANDSRPSTFKPSTSNQKERHPM
                     IKKRVGLWLSRKKAVIVSIANNIEAKRIITSDMEHYVPYSKVEPGAGNGTTENIGDLR
                     FWKHLEEYYDKIVAHISDATEIQIFGPDVAKDELQTRLGNQGLAGNIVSVEDASQMTD
                     LQIYGKVQKRFPLRSRFDLSGMNTPAPTDAEQYPLTDTDV"
     gene            6089..6409
                     /locus_tag="VFQ23_01330"
     CDS             6089..6409
                     /locus_tag="VFQ23_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905241.1"
                     /translation="MNLMNKDDFENRWQQIRAQSRDWWSLFSEDDLNKVEKATIKWDK
                     YAMMLRVKYGYTHERAKEEINKRITHLTANPPTTELMPHSAKQQTGSKSPKVRKSRTK
                     KITL"
     gene            6610..7374
                     /locus_tag="VFQ23_01335"
     CDS             6610..7374
                     /locus_tag="VFQ23_01335"
                     /inference="COORDINATES: protein motif:HMM:NF037284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905242.1"
                     /translation="MRTQKINFPDRQTLGVFPDERSNLTQAISELGLKGLNSVIVLIG
                     GTIAEPQATITRQAIETISSIAEEMNAVVICGGTDMGVMAEIGQIRSRKGYKFPLVGV
                     TPEALVTWPTGPRSTKFLWWGQQRWQLEPHFSHFILVPGSQFGDEAAWINDTARLLSK
                     DHQSVTILINGGEALRKDIELSLEYGRPVIALGGAGRLANDLSIEPDRHPLISVVPAN
                     AQQRIVEAIQSALSVHERSTVGLPSKSLAGMRKSVS"
BASE COUNT         1966 a         1790 c         1818 g         1812 t
ORIGIN      
        1 caaatcacta aaactcagat tgtcacattt tttcgtacaa cgcgaacaga tcttgagcag
       61 ggacgtttca ccggctgggc gtgatttggt agcgcctgaa gttgatggaa acgaaagcat
      121 cgttgaagat atcctacgct gggcggatga cggcggacag atgttgccac tatacattca
      181 gggtaatccc ggcaccagcc cggaatcaag cgaatatagg atcaataaaa aagctgaaga
      241 ggaaactgtc tcaaaacaat gactcaaaat ctaatatatg tgatgatttc tcaggagcag
      301 atcaataacg atggcagtta atccagtttc tcaacagtac agctggacct ttcggcgtgt
      361 cgtgggagcc acgcttgttc tcatctcggt catccttggc ttctggctag tctatcgctt
      421 caaccaggtt atttttacct tatttattgc tgtcgtgata ggcacggtga tccggccagc
      481 tgtagcctgg ctacacctgc gcgggctccc tccgacggca ggtgtcatcc ttgtctacct
      541 cttcgtgctt ctcctgctca taggctttct gctgttgtta tttccattga tcagcgagca
      601 aagttcaacg gtaacttcct ccataccgga atactatcaa aacctgcgcg tctggctggt
      661 aaatcatccc aatccattcc ttgtgggctt aagtgacttc ctccccgctg ccttaccaac
      721 cttaaacctg aggccagtgc aacaaacggg agaggagttg atggcttcag cggggcaagt
      781 cgcgggttat ataacatccg cctccaaggt gatctttaca gccataattc ttctcgttct
      841 cgctttttac tggacccttg aagggccgcg aattatcaag tcattcttgc tcgtggttcc
      901 gcaaatgcag agagagagca tcagtgacct gatttcagcc atggaaagca aggttggcct
      961 ctacgtggtt gggcaaggta tcctttgcat ggtcataggc atcatggctc tgattgctta
     1021 tacgctcatt ggcttgccaa atgctctggt gctcgccctg atcgcgggcg tgctggaagc
     1081 tgtaccgatg attggaccgt tgcttggtgc tgttcctgcg gcgctagttt cgctgtcgat
     1141 agcacctggc aagctggttt gggtcattgt cgcgacgctc gtgattcagc aattggaaaa
     1201 caccctgctt gtgccccgta tcatgaagaa agccgtagga gtaaatcctt tcgtcacgct
     1261 actggcgctt tttgccttca gttcgttgtt cggccttgcg ggagcgttga tggcgattcc
     1321 cattgcagcc atgattcaac ttgcgctgaa tcacttcgtc tttcagcagg cgacagtcga
     1381 aatggaagtc tctgagggac gtgatcatgc aagccggctt cgttatgaag cacaggattt
     1441 ggcccaggat ctgcggaagc aggcacgtct taagaaaaga ggctcagagc tagagatcaa
     1501 gcagatcgaa catgtaatgg atgagattga gacaatcaca accgacctgg acgccctatt
     1561 ggcccgaacc aataattcgg attcaaaatg acaaaacaat tactggtgat tggcactgcc
     1621 gtcatgacca ccttgctcac actggtggtg ctatggcaat ttcgtattgt agtcatttat
     1681 gttttgatct cgcttgcact tgccgcaaca gtccggccaa ttgccagcag cgaatccaga
     1741 aatagcgccg tgacacgctt gcttacgatt tttctctatg cagtcagcat cggtagcttt
     1801 ggtgtgttga tctttgtggt cggcagattg cttataagcg atcttcagga actagcccaa
     1861 attctatcta tacagaagac gtggatgttg cccccctggt tggaagggag cttgttccaa
     1921 caggcactga ctagatggtt gccagcaccg gataagatct ttgaggcaat taccagccag
     1981 cctcagctta tggtatccgc ggttctcggt tttacccagg gcgttggcgg actcgtgagc
     2041 ggttttttcg ttattctgtt tctgagcata tattggggta tcaatcaaaa ccattttgaa
     2101 cgtctctggc tctcactgct tccttccgaa cagcgcaagc atgcgcgaga catttggcga
     2161 acgctcgagc atgatctcgg ggcctacaca cgcagtgaag tcatccaaag tcttttagcg
     2221 gtgctgattt tgggcctggg ttactggctg cttggagccc catacccggc gcttctggca
     2281 gtcgttgggg cgctagcctg gctagtgccc gtagtggggg ccgctctggc tgttattttg
     2341 cccctgctgc tgggacttct gactggtgca caatttagcc tttttacggt gctttatacg
     2401 ctcattgttt taattgcgtt gcgcgtatgg atcgagccac gccttttcag acgcacattg
     2461 gataatccca ttctgaccct ggtcatactc cttgctatgg cagaaacttt tggtctgctg
     2521 ggaatcatcg tcgcacctcc gttatcggtt gtttgtcaaa ttctttggaa tctgctccgc
     2581 agtgaccgcc tggccccaga tccagcagtc caaatttcag atcttaagga acgacaagta
     2641 cgcctgcgcc ttgccctgga agagatcgaa gggccgcctt caccattagt ggtcagcagt
     2701 atggaaaggc tcaacaatct gcttgagaaa gcttcacctt ttttgccaat ggcagtatcc
     2761 aatgaacaat ccgatctgtt ccatccctcc cagcctctcg ccagcgaaga taagggggca
     2821 aacgctgaat aaacatacat taagatcgcc ttgttattct tagcctatca cgttgacccg
     2881 tattttaata tttgtagttg ccaatagtat ttctcagttc cctctcaaac gatagcaaaa
     2941 tgaagaggtc tgccaaaact cccccaagtg gcggagaaaa acactccccg gctgatggat
     3001 gcgacctggg agcatatgtt gtactataag cttagtcgaa atgtaccggt aggggtcatc
     3061 gatttccagc tttgcaactg gtgtccatat ccaaaccacc aggcatgatt gtcagtcacg
     3121 ccaggtaaat agtttttaca ttcaaaagca gcgattgata aacaaaggaa aaatgaaatg
     3181 ccaaacaaaa gaatgagcaa gtcccagttc gtcgctaccc tagccgagaa aagcggcctc
     3241 aataagaaac aagccacctc cgcgcttgat accatcaacg cgatggttgc ccagcagctc
     3301 ggcaagcgag gaccgggcga agtccttatc ccaggcctgc ttaagctgaa tattgttgac
     3361 aagcctgcca cacgcaaaca cgaaggtatc aacccattta ctaaggagcc gatgacttat
     3421 aaggcaaaag cggcgcacaa ggtgatcagg gtgagggccc tcaaagcact gaaagatgcc
     3481 atataagttc cagccagcca atcctgcgtt cgccctactg tgcggcttgc agtttcttat
     3541 cttgctggat cggaaaatta acaaaaatga atgccatgcg ccgcaatttt cgcgttgccg
     3601 ctttttacgg aaccgtgtcg atcctgctga ttctatcagc ctgtattacc ctgacaccga
     3661 atccgtctga ggctgagata ttagctgcac actgtgaggc atgtgaccag gcaactctgg
     3721 cagtcatact gacccaggaa aagaattatg ccgataatca ggcagcagct gccgccgaaa
     3781 ttgtacgcgc caatgcacaa tccactttga attcagccaa cgcagtgctg agcgcagccc
     3841 aaacccagga tcaaaacaat gccaatgtcc tggcagcgca gatagcggct accgccgaaa
     3901 ttgtgcgcgc caatgcacaa gcgaccttag attcagctag ttcaacgcag aacgccgcgc
     3961 tgacccagga tgtaattcat cagacccaaa tggcggacct ggcgacgaca ggtgcgcagg
     4021 ccctggtgaa tcagcaaaat aaagatgaac tcgccgccaa cacgatcgca atgcaacagg
     4081 cccaagccgc tgccacatcg caatggtaca ccgaccagga acgccagcgt aatgtacaga
     4141 ggcagggtcc catcacattc ctatgggtgt ggtgttttcc ggtgttcatc gtactattag
     4201 cgggtttggt tctttgggga gtctggcaac gactaaagat tcaacaagac aatcaacgta
     4261 ttctcgaaat tccccctgct gacaaattgc cagcatcaac cgctgaagtt atccattatc
     4321 cacgcgatga ttcgttgccg tatcttgaaa gtgatattgt cggtaatcgt tatttgctca
     4381 caaagccgga tgatgaggtg cgggcatggc ttgatgaagt caagaacaag ctgcgaagta
     4441 gcgatactaa taagaaggat gaagatgatt acacagacaa ttgatccgca gtatttcgaa
     4501 caaaaagctt tgcgaatcag agcccaggta tcgaccatcc tgacaaaatg gggattgctg
     4561 ccacgattca aacgctggcg attaacgcag gatgcaagta ccggaatgat tgtactgttt
     4621 ggcatactca ataacagata tattgccacc cccttcagtg actacttcga tccacgcttg
     4681 ttgcatgatc tcgccaatga actgcagctg caggtagtgt attgcaacag tgatgggctg
     4741 cgctatgcct tcatcctgga tcggggtgag ttcggcaaat taccaacgca tgtagactct
     4801 ctcttcatcg aggacggcca gcttttagcc aaggttgttt acagcgatac cccgcaattc
     4861 attccggttc cgcctgtaga ggtcgcgacg attgatgatc aaacgcgggt gcagcagggc
     4921 gtaggagctt ttttgaaggt ctttgacgat attaagctta gagatgatgc cgccttagag
     4981 ctctctgttc aaaacttgcc cgttgttgtg gttatggatg aactgaaatc aagaaacgag
     5041 tagcagagca cgaagaagcc tatgagtaga aacttaattt tgaattgcat ccccacaaga
     5101 tgaatatgga gaatttcgaa tgaagccaaa caatgatgtg ctcgaaagca agtgggatga
     5161 attgcgtata aaactcctca actggcgaaa caagcgccgc gaggatcaac agatcaaagt
     5221 caatagcgtt cgtgaaaagt caattaattt gcttcaaaaa cgctatggat attccagaga
     5281 gaaagcgata gaggaattgg acacgcgtta ttcagggacc tggctcggct gaaacaatcc
     5341 gtatctattt aaagaaagtt ggaccaagta tgacgaaaac aaacaatctc accaaatggc
     5401 tcgatatcga acttatgaat ctcgtacgcc gtaaggcaaa cgatagcagg cccagtacgt
     5461 tcaaaccttc aacctcgaat cagaaagaga gacatcctat gataaagaag agagttggct
     5521 tgtggctcag ccgtaaaaag gcggtcattg tgagtattgc caacaacatt gaggcgaaac
     5581 gaataatcac gtcagacatg gagcattatg tcccctattc caaggttgag cccggagccg
     5641 gaaatggcac aacagagaat ataggcgact tgcgtttctg gaagcatctc gaagagtatt
     5701 atgacaagat cgtcgcgcat attagtgatg ccacagaaat ccaaatcttt ggaccagacg
     5761 ttgccaagga cgaactccaa acacgtctgg ggaaccaggg actggccgga aatatcgtca
     5821 gtgtggaaga tgccagccaa atgacagatc ttcagattta tggaaaggtt cagaagcgat
     5881 ttccattgcg gtctcgattt gatctttctg gaatgaacac tccggcacca acggatgcag
     5941 aacaatatcc cctgactgat accgatgtat gaaattgaaa agtagtttgc ccaggacgag
     6001 tacggggtcc tgcatccaac gatcgaaaag catattttgc tggaaagaga ttttcaattc
     6061 ttaaccataa ccgtgaagga gtattctcat gaatttgatg aataaagatg acttcgaaaa
     6121 taggtggcaa caaatccgtg cccaatccag ggactggtgg agtctgttta gcgaagatga
     6181 ccttaataag gtagagaaag caaccattaa gtgggataaa tatgccatga tgctacgggt
     6241 gaaatacggc tacacccacg agcgcgccaa agaggaaatc aataagcgaa tcacccattt
     6301 aaccgccaat ccacctacca ctgaattaat gccccactcg gcaaagcaac agaccgggtc
     6361 gaagtctcca aaagtgcgga aaagccggac gaagaaaatt accctgtgat tctatgattt
     6421 caaaaacact gcatttgttc ttcttcgtta tagatttgct tgttttactg gcttatccga
     6481 ttgtatttgt gtacgacaaa ctacaccatg cttcagaccc caaagcaagc agccctcagg
     6541 caaatgtatg ggttcaaccc cacccgccaa ttagccgacc cgaaaagaca atgagaaaga
     6601 aaggtaatta tgcgtacgca aaagattaat tttccagacc ggcaaaccct tggtgttttc
     6661 cctgatgagc gttcgaattt gacgcaagcc atatccgaat tgggattgaa aggcctcaat
     6721 tcagttattg tattaatcgg cggtacgatt gctgagccac aagcgacaat aactcgacag
     6781 gcgattgaaa ccatctccag catcgcagaa gagatgaacg cggtggtcat ctgcggagga
     6841 acagacatgg gggttatggc tgaaatcggc cagatacgat cacggaaggg ttacaaattc
     6901 ccgttggttg gcgtaacgcc tgaagccttg gtcacgtggc ccaccggtcc gcgcagtacc
     6961 aagttcttgt ggtgggggca acagcgctgg cagttggaac ctcacttctc ccattttatc
     7021 ctggtgcccg gcagccagtt tggggatgag gctgcatgga ttaacgatac ggcaaggctg
     7081 ttatccaaag accaccagtc tgtgacgatc ttgatcaacg gcggagaagc tttacggaag
     7141 gatattgaat tgagtttaga gtatggccgc ccggtcatcg ctttgggtgg agcgggacgc
     7201 ctggccaatg acttatccat cgaacccgac cggcacccgt tgattagcgt ggtaccggcg
     7261 aacgctcagc agcggatcgt tgaagccatt caatcagcgt tatccgttca tgaaagaagc
     7321 acagtgggat taccctctaa atcccttgct ggaatgagga agtcggtgag ctaagagagc
     7381 gacggt
//
LOCUS       DASTJP010000044         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_517839, whole genome shotgun sequence.
ACCESSION   DASTJP010000044 DASTJP010000000
VERSION     DASTJP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517839"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFQ23_01340"
     CDS             <1..782
                     /locus_tag="VFQ23_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HET9905243.1"
                     /translation="KMAVNKNPTSSQKFVAEVIGTAFLVWVGPGSITTFSFISGGNLP
                     FTMSDLGWIALAFAFAIIAMIYTIGHISGCHINPAVTLAMALSKRMSWKDATSYWGAQ
                     LLGAFIGAGLIGLCYGFTNAAQLGYGATDFSEANTGYVVAILAELVGTFFLLFVIMGS
                     AVDGRAPGGFAGLAIPLAVAADILVFGPVTNVSLNPFRSLAPAVWQVAFGGTYNMAHL
                     LVYFIGPALGAVLGVWVYDFITREEPAKASVLRETAVKADR"
     gene            807..1808
                     /gene="dhaK"
                     /locus_tag="VFQ23_01345"
     CDS             807..1808
                     /gene="dhaK"
                     /locus_tag="VFQ23_01345"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HET9905244.1"
                     /translation="MKKLINKPEDVVVEELQGLELAHADLLRVCYEPNFIVRVDAPVR
                     GKVGVISGGGSGHEPMHGGFVGLGMLDAACPGAVFTSPTPDQMLEATKAVNGGAGVLH
                     IVKNYTGDVLNFEMAADLARAEGIEVEAIVTDDDVAVKDSLYTAGRRGVGVTVLLEKI
                     VGAAAEQHRPLKDVAELARKVNANGRSMGMALSSCVVPQAGKPTFDLPDDEMEIGIGI
                     HGEPGRTRTKLGTAKEITEMLATPILDDLPFSNGDSVLAFVNGMGGTPLIELYVVYNE
                     LAKICAGRGVKIARSLVGSYITSLEMAGCSITLLKVDDELTRLWDAPVKTPALRWGI"
     gene            1904..2542
                     /gene="dhaL"
                     /locus_tag="VFQ23_01350"
     CDS             1904..2542
                     /gene="dhaL"
                     /locus_tag="VFQ23_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HET9905245.1"
                     /translation="MTISRDQFIVWVKDFAGVLAENKEYLTGLDSAIGDADHGINMDR
                     GFQAVLTKLPAFENQDIGSIAKNIGMVLISTVGGASGPLYGTLFMQLGKETAGKLELT
                     LNDWITALDSAVNGVMMRGKANLGDKTMLDTLIPALNKLKECAATGASLEDALREAEK
                     TAEQGMLSTVPLVARKGRASYLGERSAGAQDPGATSSYYLLKTAAHALGRSA"
     gene            2573..>3247
                     /gene="dhaM"
                     /locus_tag="VFQ23_01355"
     CDS             2573..>3247
                     /gene="dhaM"
                     /locus_tag="VFQ23_01355"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HET9905246.1"
                     /translation="MIGIVIVSHSAKLAEGVVELARSMGGADLSIQAAGGMSEDTTLG
                     TDPIRILKAIEQVYSDDGVLVLMDLGSAILSSEMALEMLAEEQRGKVVLCEAPLVEGA
                     IAAAVQARIGGTIQQVIQEARSALTPKSTHLNTVSHEPNPAQSAISSENQLAIQLTVN
                     NELGLHARPAARFVQTAGRFPQESILVSNLTTKKGPVNAKSINSVITLGVRQGHQIEV
                     RASGPNA"
BASE COUNT          751 a          790 c          932 g          774 t
ORIGIN      
        1 agaaaatggc tgtcaataag aatcccacaa gtagtcagaa gtttgtcgct gaggtgatcg
       61 ggacggcgtt cctggtctgg gtcggacccg gctccatcac tacctttagc tttatctcag
      121 gtggcaatct gcccttcaca atgtctgacc tcggctggat cgccctggcg tttgccttcg
      181 ccattattgc gatgatctat accatcggtc acatttcggg atgtcatatc aaccccgcgg
      241 tgaccctggc gatggcgtta agcaagcgca tgtcctggaa ggatgccaca tcctattggg
      301 gcgcgcagtt gctgggagcg ttcatcggcg caggtttgat cggactctgc tatggtttta
      361 cgaatgcagc acagcttggc tacggcgcaa ctgacttcag tgaagccaat acaggctatg
      421 tggtcgcgat cctggcagaa ctggttggca cgttcttcct gctgtttgtg atcatgggct
      481 ctgccgtgga tggtcgcgca ccaggcgggt ttgccggtct tgccattcct ctggctgtgg
      541 cggcagatat cctcgtcttt ggaccggtga ccaatgtttc cctcaacccg ttccgctcac
      601 tggcgcccgc tgtgtggcag gtggcatttg gtggcaccta taacatggcg cacctgcttg
      661 tctatttcat cggaccggca ctcggggctg ttctgggtgt ttgggtgtat gatttcatta
      721 ctcgtgaaga accggcaaag gccagtgttc tgcgcgagac agctgtcaaa gctgatcgtt
      781 gatcgcagcg tcataaggag aagaacgtga aaaagcttat caacaaacct gaagatgttg
      841 tcgtcgaaga gctgcagggg ctcgaactcg ctcatgcaga tctcctcaga gtgtgctacg
      901 aaccaaattt tattgtccgc gtggacgcgc cagtgagagg caaagtgggc gtaatttccg
      961 gtggtggttc aggacatgaa cccatgcatg gcggatttgt cggccttggc atgctggatg
     1021 cggcttgccc gggtgcggtc tttacctcgc ccacgcccga tcaaatgctc gaagccacca
     1081 aagctgtcaa tggcggagcc ggtgtgttgc acatcgtcaa aaactacacc ggtgatgtct
     1141 tgaactttga aatggctgca gacctcgccc gtgcagaagg aatcgaagtg gaagccattg
     1201 ttacggatga cgatgtggct gtgaaagata gtttatatac ggcgggcagg cgcggtgttg
     1261 gcgtgaccgt gctgctcgaa aaaattgtgg gtgcggcagc cgaacagcat cgtccgttga
     1321 aagatgtcgc cgaactggcc cgaaaggtca atgccaatgg ccgcagtatg gggatggctc
     1381 tctcctcctg tgttgtgcct caggccggaa agcccacctt tgatcttcca gacgatgaaa
     1441 tggaaattgg tatcggtatt catggcgaac cagggcgaac acgcacgaag ctcggtacag
     1501 ccaaagagat tactgaaatg ctggccaccc cgatcctcga tgaccttcca ttcagtaacg
     1561 gcgattccgt gctggcgttt gtcaatggga tgggtggaac gcctttgatc gaactgtacg
     1621 tcgtgtacaa tgagttggca aagatctgcg ccggccgcgg tgtaaagatc gctcgcagtc
     1681 tggtgggctc ctatatcacc tccctcgaaa tggctggctg ttcgatcaca cttctcaaag
     1741 tggatgatga actgacccgg ctttgggatg cgcccgtgaa gactccggca ttgcgctggg
     1801 gaatatagac agtctcggac cttgaaggag gcaggtggaa ccttttcatc tgcctccttt
     1861 gatcagataa cgtgtagtca attcatgaga aggataggct gatatgacaa tttcccgcga
     1921 ccaatttatc gtttgggtca aggattttgc aggtgtgctg gcagagaaca aggaatatct
     1981 caccgggctt gattctgcga ttggcgatgc cgatcatgga atcaacatgg atcgcggctt
     2041 tcaagcggtg ctcaccaaac tgcctgcctt tgaaaaccag gatatcggtt cgattgccaa
     2101 gaatattggc atggtcctga tctccacggt cggtggtgcc agtgggccgt tatatggaac
     2161 tctctttatg caactcggca aagagacagc cggaaaattg gaattgacct tgaacgattg
     2221 gatcacagcc cttgattcag ccgtgaatgg agtgatgatg cgcggcaaag ccaacctggg
     2281 ggataagacc atgctggata ctttgattcc tgcactaaac aaattaaaag aatgcgcagc
     2341 cacgggcgca tctctggaag atgcgcttcg tgaagctgaa aaaactgcgg agcagggaat
     2401 gttatccact gttccgctcg tcgccaggaa agggcgtgcc agttatctgg gggagcggag
     2461 tgccggggct caagacccgg gtgccacctc ctcttactat ttattgaaga cggctgctca
     2521 tgcactgggt cgttccgcgt aacgttcaat cgttaccatt gaggaaaaca gagtgatcgg
     2581 aattgtgatt gtttcgcaca gtgcaaaact cgctgaagga gttgtggaac tggcacgcag
     2641 catgggtggc gccgacttat cgattcaggc tgccgggggg atgagtgaag ataccacatt
     2701 gggaacggat cccatccgta ttttgaaagc cattgagcag gtatattctg acgatggcgt
     2761 gcttgtgctc atggatctgg gcagtgcaat tttaagttcg gaaatggctc tggagatgct
     2821 ggcggaggaa caacgtggga aggttgtcct atgtgaagca ccgcttgtag aaggcgcgat
     2881 tgctgccgca gtccaggcac gcattggtgg cacaattcaa caagtgattc aggaagcgcg
     2941 cagcgcattg acaccaaaat ctacgcatct gaacactgta tcccatgagc caaatccagc
     3001 gcagtcggca atttcatctg aaaatcaact cgcgattcaa ttgactgtaa ataatgaact
     3061 gggcttgcat gcacgccctg cggcacgctt cgtccaaacc gccgggagat ttccacaaga
     3121 gagcattctt gtaagcaatc tgaccaccaa aaaaggtccg gtcaatgcca agagcattaa
     3181 cagtgtgatc acgctgggtg tgagacaagg ccatcaaatc gaggtaaggg cttccggtcc
     3241 aaatgca
//
LOCUS       DASTJP010000045         4754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_96704, whole genome shotgun sequence.
ACCESSION   DASTJP010000045 DASTJP010000000
VERSION     DASTJP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96704"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..940)
                     /locus_tag="VFQ23_01360"
     CDS             complement(308..940)
                     /locus_tag="VFQ23_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905247.1"
                     /translation="MIVRVVIPFYSGYYASLLDIFLLCTEPIRYMLLIGSVIVLIKIE
                     LTRKRKFLTFAVVTLLFVLGLIPTGHYLTLGALHSLDSANPEQFRNAAREMLDEYEPD
                     TLFSDNPPRNLDQTQVPRDKIPYSIVRANVRDMLVLKDYVFVEKFGLLGLFRGFIVFR
                     VGSDIWKNDKAFTLLEGCSYCWKIRVIEGLYWYHAVPTGEEISTVFHPLK"
     gene            complement(1039..1956)
                     /locus_tag="VFQ23_01365"
     CDS             complement(1039..1956)
                     /locus_tag="VFQ23_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905248.1"
                     /translation="MRVLFIFLDGIGLGANNPETNPFARAKMPNLKALLDGRSLLKDS
                     APFHGERATLVAIDPAVGVNGLPQSATGQGMLVTGINISAELGYHYGPKPNPEVAAFL
                     NDGTLFSRLVAAGKKTVLLNAYPPRYFDGIDSGKRLYSSIPLAVTNAGLELFRHEDLF
                     AGRALSADFTGMGWRTMLGFPAAPTMDPHQAGRKLVSLAMDYDFSLFEYWASDYAGHK
                     QQMETAIELMETFDGVLGGVVDEMNDEMLVLITSDHGNMEDLSTRKHTGADVPAIVIG
                     EEAAREEFTRGMMDLTHVAPAIWKMVMEF"
     gene            2335..3189
                     /locus_tag="VFQ23_01370"
     CDS             2335..3189
                     /locus_tag="VFQ23_01370"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET9905249.1"
                     /translation="MPTIDLKKLNKHRTRTFRLPPSPRVTSPKAALDFINERGFIYFW
                     PIKGIDMPSLWTAVAGNRIVADKHDDPGHVTWGWKDGALGKKTWYYAKILRKKATMIS
                     LDTVPYFYALSENYGSPEEDYLVAYEEGRLTQATKQVYEAILNEGALNTIDLRRVAKL
                     TNAKESEFNKALEVLQADFKILPIGVAEAGAWKYSFIYEIVPRHYPELPERARKIGER
                     EARSKLVELYFESVGASQERDVSKLFGWQKELITKTIASLIEKQTLIEGEHPKEKGEW
                     LILPKLIK"
     gene            3299..4210
                     /locus_tag="VFQ23_01375"
     CDS             3299..4210
                     /locus_tag="VFQ23_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9905250.1"
                     /translation="MLEISIMIEGQNGLTWPRWQKIARLVEDAGFVGLFRSDHFTNAN
                     PPDLESLELWTSLTWLADNTKRIEFGPLVTPFSFRHPAFTARMAAAVDDLSNGRLTLG
                     LGAGWQDREHQLFGFDLLEPKPRFDRFEEGMEVLTRLLQSDEPVTFEGQYFQIRGATL
                     LPRPQRRGGPRILIGGEGKRTLANVVRYAVEWNCVMLTPRKFANRNGLLTRMLSEAGR
                     QPESVRRSMMTNCIFGKDEFALQGKISARGRTLEELEGRGFIVGSANAVKEQLQELEQ
                     AGLQRIMLQWLDLDDLDGMEALAKAIL"
     gene            4322..>4754
                     /locus_tag="VFQ23_01380"
     CDS             4322..>4754
                     /locus_tag="VFQ23_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905251.1"
                     /translation="MRRRKQVIYLYILLVFDIALVSCGPDVSAAPVEPEPQKPDITVT
                     VTSESGGEISTGIARILGTPHAVTLQHNKFTLPFCSPGNYISMWSPGYYIKTVPCTNE
                     QTYMLQLKKLEQDNINYLWASAQTCAGCHSQSQGRTEHLEWT"
BASE COUNT         1257 a         1154 c         1161 g         1182 t
ORIGIN      
        1 atttggaaca gaaattgaag aattactgaa attgggactt attgagtccc cctctcctgc
       61 cgggagaggg gttaggggtg agggaaaaat attacggctc acccagcgcg gccgcctgct
      121 cggcaatcag gtctttatgc ggtttgtgtg aacaacaact ggtgggttaa gaactggctt
      181 tgtgctttat gtatctactt gctcactaac tacatcgctt gaatcgttat gctttaatga
      241 cgataactgt ctttaaactc aagacagtta ctgcacgttg tattgggcgg aattagagat
      301 ggaaggatta ttttagggga tggaagaccg tcgaaatttc ctctcctgtt gggacggcgt
      361 gataccaata caggccctca atcactctta ttttccaaca gtaactacaa ccttccagaa
      421 gcgtgaatgc cttgtcattt ttccagatgt cggagcctac tcgaaaaact ataaagcctc
      481 gaaacaatcc gagcaagcca aatttttcta caaaaacata atcttttaat acgagcatgt
      541 ctctaacatt agctctcact attgagtatg gaattttatc tctcggtact tgagtttgat
      601 caagatttct tggcgggtta tcgctaaata atgtatctgg ttcatactca tctagcattt
      661 ctctagcggc atttcggaat tgttctgggt tggcactatc aagagaatgt aaagcaccta
      721 aggtcagata atgaccagtg ggaattagcc cgaggacaaa caataaagta acgacggcaa
      781 aagttagaaa tttacgcttt cgagtcaact ctatcttgat aagaacaatt acactaccta
      841 ttaagagcat gtatcgaatt ggctctgtac aaagaagaaa tatgtctaaa aggcttgcat
      901 aatatccact ataaaaagga atcaccactc taacaatcat aagcagaact atcccaacaa
      961 gtaagaatac gagcttatga ttctttatag aacctatcat gtgttacttt tctatatttt
     1021 tctacacaga acttagtcct aaaattccat caccatcttc caaatcgcag gtgcgacgtg
     1081 ggtcaaatcc atcatgccgc gcgtaaattc ctcacgagcc gcttcctctc caatgacaat
     1141 cgcaggcaca tccgcgcccg tatgcttgcg cgtggataag tcttccatgt ttccgtgatc
     1201 ggatgtaatc agcacaagca tctcatcatt catctcatcc actacgccgc ccaacacccc
     1261 atcaaacgtt tccatcaact caattgccgt ttccatctgt tgtttatgac cggcgtaatc
     1321 gcttgcccaa tattcaaaca aagagaagtc gtagtccatg gcaagggaaa caagcttgcg
     1381 tccggcctga tgtggatcca ttgtcggtgc agccggaaag cccagcatcg tccgccagcc
     1441 cattccagta aagtccgcgg aaagcgcgcg tcctgcgaaa aggtcttcat gcctgaagag
     1501 ttccagacct gcgttggtta ccgctaaggg aatcgacgaa tacaatcgtt tccccgaatc
     1561 gatcccgtcg aagtatcggg gcggataggc attgagcaag accgttttct ttcccgcggc
     1621 aaccagtctc gaaaacaagg ttccatcatt caaaaatgca gcgacttcag ggttgggctt
     1681 tggtccgtag tgatagccca gttctgctga aatgttgata ccggtcacaa gcatcccctg
     1741 ccctgttgca gattggggaa gtccattcac gccaacggcc gggtcaattg ctactaaggt
     1801 tgctcgttcg ccatgaaacg gcgcggagtc tttcagtaat gatctgccat ccagcagcgc
     1861 tttcaaattt ggcatcttag cccgtgcaaa cggattggtc tcgggattgt ttgctcccag
     1921 gccgatacca tccaggaaga taaaaagtac tcgcatggcg gaggattata catccttcat
     1981 aaaagtatgt cattgcgacg agggctgatg gtcgagtagc cctgagcgtt cgttgcgaca
     2041 cttgaacctg catgcacacc gccccggtgg ttcggtgccg gggagtgcag gtgagggcat
     2101 atcgagaccc agcccgacga cacttgcacc gcactgcgtt cggtgcagtg caggtgagca
     2161 agctcaacta acctccaaaa gggagattgc ttaatattcg cgcttcgacc gcactacgtt
     2221 ccgctcagcg cgaagaacat cgcaacactt gcacctgcgt gcacaccgtc ccggtggttc
     2281 ggtgccgggg agtgcaggtg tgacattttt gaacggatga actaaaatta attcatgccc
     2341 acgattgatc taaagaaact taataaacat cgcacgcgga cctttcgcct gccgccgtcg
     2401 ccgcgagtta cttcaccaaa agcggccctc gatttcatca acgagcgtgg cttcatttat
     2461 ttctggccca tcaaaggcat tgacatgccc agcttgtgga ccgcagtggc agggaaccgc
     2521 atcgttgcag ataagcatga tgacccaggt cacgtcacgt ggggatggaa agatggagcg
     2581 cttggaaaga agacctggta ttacgcaaag atattgcgca aaaaagcaac catgatctcg
     2641 cttgataccg ttccttactt ttacgcgctt tcagaaaact atggttcacc ggaggaagat
     2701 tatttagtgg catatgagga aggacgattg acacaagcca cgaaacaagt ctacgaagcg
     2761 atactcaacg agggcgcgct taatacaata gacctgcgcc gcgttgcgaa actgaccaac
     2821 gccaaggagt cagaattcaa caaagcgctt gaggtgctgc aggcagattt caagattctg
     2881 ccgatcggtg ttgcagaagc cggggcctgg aaatactcat ttatttatga gatcgtcccg
     2941 cgccattatc ccgagctgcc tgagcgggcg cggaaaatcg gcgagcggga ggcgcgttca
     3001 aaattggtcg aactatattt tgaatcggtc ggcgcatcgc aggaacgcga tgtgagcaaa
     3061 ctcttcggat ggcaaaagga attgataaca aaaacaatcg ccagcttaat cgagaagcag
     3121 acgttgattg aaggtgaaca cccaaaagaa aaaggggaat ggttgatttt gccgaaacta
     3181 attaaataac gcttaccctc attgccaggg cagtgtttac ttgcccgaag caatcttcac
     3241 aaaacttgag attgcttccc ccttcgggct cgcaatgacg ataagagaaa gaggattgat
     3301 gcttgaaatc tcgatcatga ttgaagggca gaatgggttg acatggccgc gctggcaaaa
     3361 gatcgcgcgg ctggttgaag atgccgggtt tgtcggtctg tttcgttctg atcatttcac
     3421 aaacgcgaac cctcccgatc tcgagtcact ggaattatgg acctccttaa catggctggc
     3481 agataacacg aagcgcatcg agtttggtcc gctggtgact ccgttttcat ttcgtcaccc
     3541 ggccttcacc gcacggatgg ctgcagccgt tgatgatctt tccaatggac ggctgaccct
     3601 tggcctgggc gcgggctggc aggatcggga gcaccagctt tttgggtttg atctcttgga
     3661 accaaaaccg cgcttcgacc gttttgagga ggggatggaa gttctaaccc gactccttca
     3721 aagtgatgaa cctgtcactt tcgaaggtca atactttcag atccgaggcg ccacgctttt
     3781 gccgcgcccg caacgcagag gcggtccgcg tatcctgatc ggcggtgagg ggaaacgaac
     3841 gttagcaaat gtggtacgtt atgctgtgga gtggaactgc gtcatgttga ctccacgtaa
     3901 gttcgcaaat cgaaatggac ttttgactcg aatgctcagt gaagctggca ggcagccaga
     3961 atcggttcga cgatccatga tgaccaattg tatttttggc aaggatgaat tcgcgttgca
     4021 gggcaaaatt tctgcccgtg gaagaacgct ggaggaactt gaggggcgcg gctttatcgt
     4081 cggttcagcg aacgcggtca aagagcagtt acaggaactg gaacaggccg gtttgcagcg
     4141 tatcatgcta caatggctgg acctggacga tctggatggg atggaagcat tggcaaaggc
     4201 aatcttataa acacacatac accaattaat gcacccttca cgttaatgta gatatcatcc
     4261 ctatttgtgc cgtactcttc aggcagagga ggtatatctg atgagttttt taggagtcgt
     4321 catgcgacga cgcaagcagg ttatttatct gtatatcctg cttgtctttg atatcgccct
     4381 ggtttcctgc gggcctgatg taagtgctgc gccagtcgaa cccgaaccac agaaacctga
     4441 cataacagtg actgtgacat ctgaatcagg aggggagatt tcgactggca ttgctcggat
     4501 tcttggcaca ccccatgctg ttacccttca acataataaa tttactttac cgttctgctc
     4561 ccccggtaac tatatttcca tgtggtcacc cggctattac ataaaaacgg tcccctgcac
     4621 gaacgaacaa acctatatgc ttcaattgaa aaagctcgag caggacaata ttaattactt
     4681 atgggcttct gcccaaacct gcgccggttg ccattcacag tcgcaggggc gaacagaaca
     4741 tcttgaatgg actc
//
LOCUS       DASTJP010000046         4099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_834818, whole genome shotgun sequence.
ACCESSION   DASTJP010000046 DASTJP010000000
VERSION     DASTJP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834818"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..659)
                     /locus_tag="VFQ23_01385"
     CDS             complement(60..659)
                     /locus_tag="VFQ23_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905252.1"
                     /translation="MTASKFSTTKIGWVAIATGVSAILAVTFILLMYAVDMSFGKVND
                     AFNSLIGILSATLTGMLYEEQHAKSRLMNQALIVLTVIGAIFTVVGSVLIIFGFTDFV
                     LAGWYSGIGFALIGVCLAMISYSLLYDETWPQTLLVFGIVTGACMAIGLFGIPGILAR
                     IDTMDSMPWYLYVAFFGWLGTYILYPIWTIWLGRNLLRK"
     gene            complement(766..1380)
                     /locus_tag="VFQ23_01390"
     CDS             complement(766..1380)
                     /locus_tag="VFQ23_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET9905253.1"
                     /translation="MFEPEFLLTSLIVVLIPGTGAVYTITTGITLNWRASLAAALGCT
                     LGIVPHILATILGLSAILNMSAQVFAVLKLAGSAYLLYLAWNMWREAGTLDLNKTNVE
                     TSLSQIILKAIAINLLNPKLTIFFFAFLPLFVSRNTSSPTLEMILLSGVFMAITLFVF
                     ALYGILASGIRAYLMKSSEAIARLQKAFAVILAGFAVQLAASEN"
     gene            complement(1506..1964)
                     /locus_tag="VFQ23_01395"
     CDS             complement(1506..1964)
                     /locus_tag="VFQ23_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905254.1"
                     /translation="MLRAAIAEARAFESWQDYQDALKRAIMPLTDAQLQRRLLPGLRT
                     PGEIAEHIVFGRALHLHRTLGTVEELTPLLRWEDTDNPPHTAAEILQGLELTWQVITA
                     SLMRGSPTDDISEDEAPIAQTIWGLLDHDLPHAGELSLLLGADGIAGVEI"
     gene            2220..2624
                     /locus_tag="VFQ23_01400"
     CDS             2220..2624
                     /locus_tag="VFQ23_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9905255.1"
                     /translation="MTETTPFLMFCGPHKGQAEEAITLYVSLLKSSQIIAIDRYGANE
                     HEPEGTVRLARFTLAGREYMAIDSAMEHNFTFTPAFSIFVTCEDETELETLYETLGAR
                     GAVLMPLGNYGFSRKFGWVNDRYGVSWQMNLP"
     gene            complement(2694..3257)
                     /locus_tag="VFQ23_01405"
     CDS             complement(2694..3257)
                     /locus_tag="VFQ23_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9905256.1"
                     /translation="MAHLIYSAIASLDGYIEDADGNFDWAMPDEEVHTFINNLERTSG
                     TYLYGRRMYETMAVWETDPNLAADSPLTQDFAQIWQAANKIVYSRTLTAASTRNTQIE
                     RNFNPEAIRQLKKTVQHDISIGGPELAAHAFQSGLIDECHLFLTPILVGGGKRALPDN
                     VRLELGLLEERRFDNGMVFLRYQARHG"
     gene            complement(3378..4031)
                     /locus_tag="VFQ23_01410"
     CDS             complement(3378..4031)
                     /locus_tag="VFQ23_01410"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HET9905257.1"
                     /translation="MTTQDSAHPQIVSRNEWLVARETLLAHEKELTKHQDKVSAERRR
                     LPMVKVDKTYTFNGSEGKRSLYDLFDGQSQLIVYHFMFDPSWDDGCMGCTGFVNDLGD
                     LSELQVRNTQFAMISRAPLEKLLAYKTRMGWERPWYSAFDTDFNQDFQVTVDGGERHG
                     LSVFFRIDDDVFHTYSTYNRGCESLSDVYRLLDMTPYGRQEDWEDSPAGFPQRPTYG"
BASE COUNT         1083 a          991 c         1008 g         1017 t
ORIGIN      
        1 ctccttagtt ggattgaaga aaatttatag ctcgcttgtt ctgtttatgc atggttcctt
       61 catttgcgga gcagatttcg ccccagccag atcgtccaaa tcggatacag gatgtatgtt
      121 ccgagccacc caaaaaacgc aacataaaga taccacggca tggaatccat ggtatcaatt
      181 cgagctaaga ttcccggaat accaaaaaga ccaatcgcca tgcaggctcc tgtgacaatc
      241 ccgaaaacga gcagggtttg cggccaggtt tcatcataga gtaacgaata agaaatcatt
      301 gccaggcaca cgccgatcag agcaaatccg atgcctgaat accacccagc cagcacaaag
      361 tctgtgaagc cgaaaatgat caggactgat ccgacgacgg taaagatggc gcctatcacc
      421 gtaagaacaa tcaaagcttg attcatcaac cgtgatttgg cgtgctgttc ttcatataac
      481 atccccgtca gagtcgcgct taagataccg atcaggctgt tgaaagcatc attgactttg
      541 ccaaaggaca tatctaccgc gtacattaag aggatgaatg tcactgcaag gatagctgaa
      601 acgccggtgg cgattgccac ccatccaatt ttggtagtgg agaatttgct ggctgtcacg
      661 gatctttcct aatttactaa aataggccgc ctcgattcgg aagcagacca acggtttgtg
      721 gctgagaagc aatgacggaa tttttgggtc acgatattgg acgcgtcagt tttcgctggc
      781 tgccaattga acagcaaaac cagccaggat cacggcaaac gccttttgaa ggcgtgcaat
      841 tgcttcggag gacttcatca aataggcacg aatcccactg gccaagattc catacaaggc
      901 aaacacaaat aaggtgatcg ccatgaatac cccacttaac aggatcattt ccagggtggg
      961 agacgatgta ttcctggaga cgaacagcgg aagaaacgca aaaaagaaaa tcgtcaattt
     1021 cgggttgagc agatttattg caattgcctt taggatgatt tgtgaaaggc tcgtctctac
     1081 attggtcttg ttaagatcca atgtacccgc ctcacgccac atattccagg ctagataaag
     1141 caaataggca gaccccgcta gtttcaagac tgcaaagacc tgtgcgctca tattcagaat
     1201 tgccgagaga cccaggatag ttgccaaaat atgaggaacg ataccaagtg tgcatcctaa
     1261 agcggcggcc aggctggccc gccagttgag ggtaatcccg gtggtaatgg tgtaaacggc
     1321 gccggttcca ggaattagca caacaatcag cgacgtgagc agaaactcgg gttcaaacat
     1381 taatatctcc agtaataatc agaatgcctg aacggcgcgc gtgagtggtt tggcataaga
     1441 accaaatccg cagcacgcat gttagacgat ctttaaggtc ggtcttattt tacaagtcct
     1501 agcagctata tctctacccc ggctatgcca tccgcaccca acagcaacga tagttcccca
     1561 gcgtgtggca ggtcgtggtc gaggagaccc caaatcgtct gcgcgatcgg cgcctcatcc
     1621 tctgagatat catcagtcgg tgatccgcgc atcagagagg ccgtgatgac ctgccaggtt
     1681 agttccagcc cctgcagaat ctcggcggcg gtatgtggtg ggttgtcggt gtcctcccac
     1741 cgcagcagcg gcgttaattc ctcaaccgtg cccagtgtgc ggtgcagatg cagagcccgc
     1801 ccaaagacga tgtgttcggc aatttctcca ggggtgcgca gtcccggtag aagccgtcgc
     1861 tgaagttgtg catcagtgag aggcataatt gcacgtttga gtgcatcctg atagtcttgc
     1921 cagctttcga aggcacgcgc ctccgctatg gctgctcgta gcatggactc tattcctttc
     1981 ggatttgatc ttctccgttc aaaactatta ccggtgactt atgctgggag gtctttcaag
     2041 tcattgctca atagctgcca ttgaaatgat acatctggaa tagcgaacaa gtgatgcgga
     2101 acgctaaaat attcaccgat tgactgacac acatagcaag cttatcccag gcagaaaacg
     2161 tttatcatat tatcataaat ccgtgtcagc gcttcagacg atgaatcagg agataaagta
     2221 tgacagagac cacacccttt ctcatgtttt gcggaccaca caaggggcag gcagaggaag
     2281 caatcacact gtatgtttcg ttgcttaaga gctcgcaaat catcgccatc gatcgctacg
     2341 gcgcgaacga gcatgagccg gaaggtacag tgcggctcgc gcgttttacc ctcgccgggc
     2401 gtgagtacat ggcaattgac agtgctatgg aacataactt tacattcaca ccagctttct
     2461 ccatctttgt gacatgcgaa gatgaaacag aacttgaaac cttgtatgaa actttgggtg
     2521 cacgcggagc agtgctgatg ccattgggca actatggatt cagccgtaag tttggatggg
     2581 tgaatgatcg ttacggtgtg tcgtggcaaa tgaatctgcc gtagatagac taccgatgtg
     2641 cttctgtgat tcgcctcgag tgaagcgagt gcgtagatgc ggtgtgagat ggctcatccg
     2701 tgccttgctt gatagcggag gaaaaccatg ccattgtcaa agcggcgctc ctccaaaagc
     2761 cccagctcca atctgacatt gtcagggagg gcccgttttc ctccgccgac gaggatcggt
     2821 gtgaggaaaa gatgacattc gtctatcaat ccggattgaa aggcatgggc agcaagctct
     2881 ggaccaccga tcgagatatc atgttggacc gtctttttga gttgtcgaat tgcttccgga
     2941 ttgaagtttc gctcaatctg cgtatttcga gtggatgcag ccgtcagtgt tctggagtat
     3001 acgattttgt tggctgcctg ccatatttgc gcaaagtcct gtgttagtgg ggaatcggcg
     3061 gctaaattcg ggtcggtctc ccaaaccgcc atggtctcat acattcgacg tccgtagaga
     3121 taagtgccgg atgtccgctc aaggttattg atgaacgtgt gcacttcctc atcgggcatt
     3181 gcccaatcaa agttgccatc cgcgtcctcg atgtagccgt cgagtgatgc gatagccgaa
     3241 taaatcaaat gagccataaa actccttttg gatatgtaat tcacactgtt tcaacgaaaa
     3301 gtgtgaatgg atgaaaagcc acctaacggc tggctttacc tatgccggac agttttcaac
     3361 agaatccagc gaaaatctta gccgtaggtc gggcgctgtg gaaagccggc gggcgaatct
     3421 tcccaatctt cctgtcgtcc gtagggagtc atatcaagca atctataaac atcacttagc
     3481 gattcgcaac cacgattgta ggttgagtag gtatgaaata cgtcatcatc aatgcggaag
     3541 aaaacgctga gtccatgcct ttcaccccca tcaactgtca cctggaagtc ctgattgaaa
     3601 tctgtgtcaa atgctgaata ccagggccgt tcccagccca tacgggtctt atacgcgagt
     3661 agtttttcta atggggcacg cgagatcatt gcaaactgtg tgttccgcac ttgcagttcg
     3721 gataaatcac ccaaatcgtt tacaaatcca gtacagccca tgcagccatc atcccacgaa
     3781 ggatcgaaca tgaagtgata cacaatgagt tgactctgcc catcgaacaa atcgtaaagg
     3841 ctacgttttc cttctgaacc gttaaaggta tatgttttat ctacctttac catgggcagt
     3901 ctgcgacgct cggcactgac tttatcttga tgtttggtga gttccttttc atgggcaagc
     3961 aatgtttccc gagcgaccaa ccattcattt ctacttacaa tctggggatg tgcagaatct
     4021 tgagttgtca tattttgtcc tgtcttgacg cgattaagat aagagtgatt ttagaatatg
     4081 cgttctattt cgtcaagac
//
LOCUS       DASTJP010000047         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1468963, whole genome shotgun sequence.
ACCESSION   DASTJP010000047 DASTJP010000000
VERSION     DASTJP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468963"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFQ23_01415"
     CDS             complement(<1..337)
                     /locus_tag="VFQ23_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905258.1"
                     /translation="MKCGSIFFLNPFALLDAALSYPSNRYLALILFSIIFSSCAEPPT
                     PAPLNMAVVDIPKPEKTQQVQPPETMPTATAAHQPNAESLNASIWVPPYLAETMGGSL
                     EEPLKGLIVS"
     gene            533..988
                     /locus_tag="VFQ23_01420"
     CDS             533..988
                     /locus_tag="VFQ23_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905259.1"
                     /translation="MSFQQKNVTVSLVNFSLILAYFLSRILQMVRTGSFEPTNVFRLW
                     GIVIALAIIVTIFATILTHIVLAILHAIQTGDEKSNVEDIEDERDKLIDLRGTRLTYF
                     VYSSGAFLAMLTFVQGQPPLVMFSLLIFFGVLAQIFGDVLRLVLYRRGF"
     gene            990..1220
                     /locus_tag="VFQ23_01425"
     CDS             990..1220
                     /locus_tag="VFQ23_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9905260.1"
                     /translation="MEKRRINNNIRKLRFFQNEMTQQELAQKVGVTRQTIIAMEQGKY
                     SPSLELAFRIALVFGVPLEEVFTYELDDENMK"
     gene            1412..2131
                     /locus_tag="VFQ23_01430"
     CDS             1412..2131
                     /locus_tag="VFQ23_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9905261.1"
                     /translation="MNINRKTAITVGLLYIIGTVAGILSRVYTAPILKSSDYLSQISM
                     DPNQIVIGALFVLTMGLALAMVPVMMFPILKNYNRALAMGYVVFRGALEAVIYLFFVI
                     GWLALPLISQEYANAGAAEASYFQALGDIILKTNDQIGHVLSIVFILGALMFYSVLYQ
                     SKLVPRWLAGWGLLAATPYFIYGVLSLFTLPNSMGTLPEGLVIPLALQEMVLAVWLIA
                     RGFNISSPVTFGATNMAASQA"
     gene            2272..2583
                     /locus_tag="VFQ23_01435"
     CDS             2272..2583
                     /locus_tag="VFQ23_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9905262.1"
                     /translation="MSKTISSGTVHKMPTDLRNALTSDQAALAIWEGLTPLARNEWIC
                     WVTFVKKDETRKEHVKRTVSELKEGMRRPCCWLGCIHRKDKPISPSVQAVLNRRTKKS
                     K"
BASE COUNT          707 a          651 c          672 g          692 t
ORIGIN      
        1 cactcacaat caaccctttc aatggctcct cgagggagcc gcccattgtt tctgccagat
       61 acggaggtac ccaaatggag gcattgagac tctcagcgtt cggctgatgc gcagcagtag
      121 cagtcggcat ggtctcgggt ggctgtacct gctgtgtttt ctcaggcttg ggtatgtcaa
      181 cgacagccat gttgagtggc gcgggtgttg ggggttctgc acaacttgag aaaatgatgg
      241 agaataagat cagggccagg taacgattcg agggatagct caaagccgca tccagtagag
      301 cgaatggatt aagaaaaaag atggaaccgc atttcactcc tgattttacc gcccggctca
      361 cgcatcttgt acacgtctaa gtaaattttc acgggcatcg aaagcgtcaa ttctaacgta
      421 agtactattt ttgcattttg atgtaaaaaa tacttgacat ttagtatgat ttatattact
      481 atatgtaaag aattaaatac ctaatgtaaa tccaaacaga cacgaggaaa ccatgtcatt
      541 tcaacaaaaa aacgtcaccg tctcgttagt gaacttcagc ctgatactgg cctacttcct
      601 aagtcggatt cttcaaatgg tccggactgg aagtttcgag ccgaccaatg tatttcgcct
      661 ttggggcatt gtcatcgccc tcgcaattat cgtgacaatt ttcgccacga tcctgaccca
      721 cattgttctg gccatcctcc atgccataca aacgggagac gaaaaatcga atgtggagga
      781 tatcgaagat gaacgagaca agctcatcga cctgcgaggc acaagactca cttattttgt
      841 gtattcctct ggcgcttttc tcgcgatgct gacctttgtt cagggtcagc cgccgttggt
      901 gatgttcagc ctgctcatct tctttggcgt gctggcgcag atctttggtg acgtattgcg
      961 gctggtgctt taccgcagag gcttctaaga tggaaaagcg ccggatcaac aacaacattc
     1021 ggaaattgcg ttttttccag aatgagatga cccagcagga gctggctcaa aaagttgggg
     1081 tcacgcggca aacgatcatt gccatggagc agggaaaata ctccccttct ctcgaactgg
     1141 catttcgcat cgcgctcgtt tttggtgttc cactggaaga agtatttacg tacgagttag
     1201 atgatgagaa tatgaaataa agtaattctg ttttagtttc aaaggcaatc cgttggtaga
     1261 gtacggcgaa gccggtatcg agaccaaaag taccactcta acaaaagatt tgcttgagag
     1321 ctgtggtctc aatacgtccc acgggaatca tgcgggacta ctcgaccacc gaacctcgcc
     1381 aatcaaattc aacaaacaac aaggagcaag aatgaacata aatagaaaga ctgcaataac
     1441 cgtcggtttg ttatacatca ttgggaccgt cgcaggcata ttaagccgcg tttacacagc
     1501 gcccatcctg aagagttcgg actatctctc tcaaatttcg atggacccga accaaattgt
     1561 gatcggcgct ctctttgttc tcaccatggg gctggcactg gcgatggtgc ctgtgatgat
     1621 gtttcccatc ttgaaaaact ataaccgagc tctggcgatg ggttacgtcg tcttcagagg
     1681 cgcgttggag gctgtaatct atctcttctt tgtcatcggc tggctggcgc tgcccttgat
     1741 cagccaggaa tatgctaatg ccggtgcagc agaagcttcc tactttcaag cgttgggcga
     1801 tatcatcctc aagacgaatg atcagatcgg tcatgtactg tcgatcgttt tcatcctggg
     1861 cgctttgatg ttctactctg tgctgtatca atcaaagctc gtgccgcgct ggttagccgg
     1921 ttggggtctg ctggcagcca ccccctattt catctacggt gttctaagct tgttcactct
     1981 cccgaattcg atgggaaccc tcccggaggg gttggtcatc ccgctggcct tgcaggagat
     2041 ggtgctggcg gtatggctga tcgccagggg attcaacatc tcctcccctg tcacattcgg
     2101 agcgacaaac atggctgcaa gccaggcgtg agagatgcac ttcaattcgc aggttcgata
     2161 ataagaaaga cccgtcattt gtggcgggtc tttatcgatg tgactcttgt atagaaagaa
     2221 gcgtccaatc ggcccagcat ccagaatccg acatcttggt aaaatcatac gatgagtaaa
     2281 acaatttcat ccggcactgt ccacaaaatg cccacagatt tacgaaacgc tctcacttca
     2341 gatcaagcgg cactagcaat ctgggagggt ctaacccctc tggcgcgcaa tgagtggatt
     2401 tgttgggtta ccttcgtcaa gaaagacgag acgagaaaag agcatgtcaa acggacggtc
     2461 agcgaactca aggaaggcat gcgccggccg tgctgctggt tgggctgtat tcatcgcaag
     2521 gataagccga tcagcccttc ggtacaagcc gtgctcaata ggcgtacgaa aaaatcaaaa
     2581 taagacctgc cgcgtaccga tccagaaagg ggagattgtt ttttcataat atcattcgtg
     2641 atactcttca gcacctgggt agtgacgaaa aacaataacg gctagaatca gaatcaattg
     2701 cctaacggct ggcgttcgcg aa
//
LOCUS       DASTJP010000048         6239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1363209, whole genome shotgun sequence.
ACCESSION   DASTJP010000048 DASTJP010000000
VERSION     DASTJP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363209"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /gene="mltG"
                     /locus_tag="VFQ23_01440"
     CDS             complement(<1..956)
                     /gene="mltG"
                     /locus_tag="VFQ23_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET9905263.1"
                     /translation="MRNFIIIVFLFLALACLFSALIYLPSQATRIYGPPSSALGLLER
                     IQYSALLLWYDGLLTRPVDLSGTEQTFTIEQGETVDAIANHLQIVGLIRDAESFRAYL
                     VYSGLDTSIQAGEYKISTAMSAIDVAHELQDATPEDVTFVILPGWRIEEIAASLSTSG
                     LSITPEEFISAAHTPPTGFDFLVGEQTTEGFLYPDTYVIQRESSVDQLVHEFVRNFGL
                     RISSDMRSAIDRQGYTIHNAVTIASLVEREAVHDEEKPLIASVFLNRLKIGMKLDSDP
                     TVQYALGYDILAQTWWKNPLSLQDLQFNSPYNTYIYVGLPPSP"
     gene            complement(970..1386)
                     /gene="ruvX"
                     /locus_tag="VFQ23_01445"
     CDS             complement(970..1386)
                     /gene="ruvX"
                     /locus_tag="VFQ23_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET9905264.1"
                     /translation="MRILAVDHGEKRIGLALSDPTATIASPFKVIEHISRPLDAAQVA
                     NLAAENEVGLIVIGQSFDEEGKPNLAGRRAAKFADALKAQTQIPVLLWDESFSTQDAR
                     ATRIQMGVSRKKRAGHLDELAAVMILRSYLESYIQK"
     gene            complement(1538..3031)
                     /locus_tag="VFQ23_01450"
     CDS             complement(1538..3031)
                     /locus_tag="VFQ23_01450"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HET9905265.1"
                     /translation="MKTQIITLESHDDLISVRDRMSWAKTPRILLVWPKYEKVLLRQV
                     DLKILQRHAAFLGAQLGLVTRTRHVRAEAESLRIPVFESTGQAQKVSWPAPRRRRWIQ
                     RAPRKDLREKRELVQVEEPAWRVNPLLRLGVFSVGVLSVLVLVALFIPRAQVRLTPIS
                     TTQSVVLLVIASLSVDSVFISGNIPAREKHVIVEGEQTVTVTGEGVVPQSKARGVVEF
                     RNLTQQSVTIPAGTVIQAAQDAPVRFVTLEDSAVDAGVGKTAEVPVEAIEGGFAGNLP
                     EDTLIVIEGRLGLSLSVTNPEPTTRGRERASVQASESDRARAKNLLMRSLDEMAREKL
                     RDEIGGGDMLLDETLRVSQVLLEEYNPPAGAAGTRLSLTMQVEYSVMYAAASDLAELA
                     LLAMNASLPTGFRADSADAVTINPLSKPSIEENGTARWTMKVEREIIQTVDPTQVTYL
                     IQGLSSASAQSRLQENIPLAASPIISLSPSWWPWVPIVPFRISVETE"
     gene            3155..4744
                     /locus_tag="VFQ23_01455"
     CDS             3155..4744
                     /locus_tag="VFQ23_01455"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9905266.1"
                     /translation="MTGPADFIITNAKVFTSDETHPHAEAVAIQGNRILSVGSNQDVL
                     SLQSKNTRVIDGNQKTLMPGFIDSHFHLLSGCETLGDAQLQTVKNKAELRSALQSHAN
                     ENKTATWISGIGTKYKIVSTRQELDEIISDRPVYIEAYDGHTGWANTKALELAGILED
                     GKTTGPNGVIVKDDSGVATGELREPDAINPVASLIPTPDAYRKRELLKSGIQQINAAG
                     VTSIHNMNGNMEDLMIYAALEDAGEISLRVYVPYSVRPHTTEAMLVEAVKMSEVQGEY
                     VRGGAVKFFMDGVWESYTALNIEPYADNPNANSPGIYSAEHFTRMGIACDKLGLQIFV
                     HCCGDGAVRRTLDGYEAIQKINGKRDSRHRVEHIEVIQEDDLPRFKELGVIAAMQPLH
                     APLTMNGDVWLDRTGSSRWSRSFAWRSIKDAGAILALGSDWTVASFNPMLGVHAALNR
                     QKWSPEDPDQRLTLEEVLIGYTRDAAFAEFQEHQKGILREGYLADLVLLSENIFEIPH
                     AEMANVKLVLTMANGKIVYEA"
     gene            4761..>6239
                     /locus_tag="VFQ23_01460"
     CDS             4761..>6239
                     /locus_tag="VFQ23_01460"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9905267.1"
                     /translation="MSSKADLAIINARIFTSDETIPYAEAVAIEGKHILYVGNNTGAQ
                     KFCDDKTRVIDGQGHTLTAGFIDTHVHLLSGATWMGYAELSLASTKNDLKGILREYAD
                     ANQSEEWIIGWRSPYNVISTRGELDEIIPDRPVYVRAADAHTAWANTKALEIAGLLKG
                     NRTDLPDGIVLDENGLPTGELREGKAMRAVLDFIPPPSEARIRDQLKRAIQGFNRTGI
                     TSIHNMNGDMNDLMVYAAAEDAGELNLRVYVPYSVRPETREEDLSEASKMAAVQMDYV
                     RGGLAKFFMDGVWESYTAFNIEPYADNPEISVDPIFSLEHFTRMAAACDRLGLQIAVH
                     CCGDGAVRQTLDGYEALQKMNGRRDSRHRIEHVEVCQPEDMPRFRDLGVIAAIQPSHA
                     SLKLDDYKIGQVRLGEKRWPLWQPWRDLKNAGAHISLGSDWTVVPYDPMTHFYVALNR
                     EPYSPDSTDQRLTLAECILGYTRDAAYAEFKENEKGQVKEGYL"
BASE COUNT         1635 a         1567 c         1603 g         1434 t
ORIGIN      
        1 ggcgaaggag gtaaaccaac gtaaatatag gtgttatacg gtgaattgaa ctgcaaatcc
       61 tgcaggctca acggattctt ccaccaggtt tgtgcaagga tgtcgtatcc gagcgcgtat
      121 tgcacagtgg ggtcactatc cagcttcata ccaattttca ggcggttgag aaacaccgat
      181 gcaatcaacg gtttctcctc atcatgcaca gcttcccgtt caaccagcga tgcgatggta
      241 actgcattgt ggatggtata cccctggcga tctatggcac tcctcatatc tgaagaaatc
      301 cgcaagccaa aattgcgaac aaattcatgg acaagttggt cgacagaaga ttcacgttga
      361 ataacatagg tatccgggta taggaatcct tccgtcgttt gctcgccgac caaaaagtca
      421 aacccggtgg gcggggtatg tgcggctgag atgaactctt cgggagtgat cgataagccg
      481 gaggttgaga gtgaggcagc gatctcctct attcgccagc caggcaggat aacaaaagtt
      541 acgtcctcgg gagtggcatc ctgcaactcg tgagcaacat caatggcaga catagccgta
      601 ctgattttat attcgcccgc ttgaatggac gtgtccaagc ctgaataaac aagataggca
      661 cgaaacgatt ctgcgtccct gatcaaccca acaatttgca gatgattggc aatcgcatcc
      721 accgtttcgc cttgctcgat cgtaaaggtc tgctccgttc cactcaaatc cacagggcga
      781 gtgagcagtc catcgtacca aagcagcagt gcggagtatt gaatgcgctc taacagtccc
      841 aatgcagagg aaggcggacc gtaaatccgt gtggcttgtg atggtagata gatcagggct
      901 gaaaataagc aagccagagc cagaaaaaga aaaacaataa tgatgaaatt acgcattaag
      961 gaataagagt tacttttgga tgtacgactc aagataggac ctcaagatca tgacggcagc
     1021 aagttcatcg agatgccctg cacgtttctt tcgtgaaaca cccatttgga tccgggtagc
     1081 acgagcatcc tgagtgctga atgattcgtc ccatagcagg acaggaattt gtgtttgtgc
     1141 tttcaaagca tccgcaaact tcgcagctcg tctgcctgcc agattgggct ttccttcttc
     1201 gtcaaaggac tgaccgatca caatgagccc tacttcgttt tcagcggcaa gattcgccac
     1261 ctgagcggca tccagagggc gcgagatgtg ttcgatcact ttgaacgggc tggcgatggt
     1321 tgcggtcggg tcactgagtg ccagacctat tctcttttcg ccgtggtcta ctgccagaat
     1381 tctcataaat actccgttgg ttgagtaggg cgagtgctta catcgcccgt atcgaaacca
     1441 acaagttaca aaattaattc tgtaataatg actgttttgc ctagttgtgg tttcgatatg
     1501 cccttcgcta tggcttcggg ctactcaacc accgagatta ttcagtctcc accgagatgc
     1561 gaaatggcac aatcggcacc catggccacc atgacggcga aagagagatg atcggtgatg
     1621 ccgctagcgg aatgttctct tgaagacggg attgcgcaga ggctgaactc aatccctgaa
     1681 tgagataagt cacttgcgta gggtcaacgg tttggatgat ctctcgctcc actttcatcg
     1741 tccaacgcgc ggtgccattt tcctcgatcg atggctttga aagaggatta atcgtgactg
     1801 catctgctga atcagcgcga aagcctgtgg gaagagacgc attcatcgcc aacagggcaa
     1861 gctccgctaa atcagaggca gcagcataca taacagaata ctccacctgc atggtcaggc
     1921 ttagcctcgt acctgctgca ccggcaggcg ggttatattc ttcgagaaga acttgtgaaa
     1981 cgcggagtgt ttcgtcaaga agcatatcgc cacctccaat ttcatctcta agtttttcgc
     2041 gcgccatttc atccagactc ctcatcaaaa gattctttgc tctcgcacga tcagattcgc
     2101 tggcttgcac cgaggcacgt tcgcgtccac gtgttgtcgg ctccggatta gtcaccgaaa
     2161 gagaaagccc cagccggcct tcgatcacga tcaatgtatc ctcaggcagg ttaccagcaa
     2221 agccaccttc tatcgcctct acgggcactt ccgctgtctt gcccaccccg gcgtcgaccg
     2281 cgctatcctc taatgttacg aagcgcaccg gagcatcctg cgctgcttga atcaccgtgc
     2341 cagcagggat agtcaccgat tgttgtgtga gatttcgaaa ttctacaacc cctcgcgcct
     2401 tagattgcgg gactacgccc tcgccggtaa ccgtcacagt ttgctcaccc tcaacaatta
     2461 catgtttttc gcgtgcaggg atatttccgc taatgaaaac tgaatctacg gataggctgg
     2521 caatcacgag caacacaaca ctttgcgttg tggatatcgg cgtcaatctg acttgcgcgc
     2581 gcgggataaa gagggcgaca agaaccaaga ctgatagaac tcctaccgaa aacaccccaa
     2641 gacggagcag cggatttaca cgccatgcag gttcttccac ctgaaccagc tcccgcttct
     2701 cacgcaggtc cttgcgtgga gcacgctgga tccatcttct tcgtctgggt gctggccagg
     2761 aaaccttttg cgcctgcccg gttgactcaa acaccggaat gcgcaacgac tcggcttctg
     2821 cgcggacatg ccgcgttcgt gttaccaatc ccagctgtgc gcccagaaag gctgcgtgcc
     2881 gctgtaggat tttcaaatcc acctgcctga gaaggacctt ttcatacttt ggccaaacca
     2941 gcaaaatacg cggcgtcttc gcccaggaca ttcgatcgcg gactgagatc aggtcgtcgt
     3001 gtgattcaag cgtgatgatc tgagttttca ctctcaggat tataccgaaa gacctccaaa
     3061 tcatcttgac ctgccttggg tctttatgta tatttacacg atccagactt cggaagtctc
     3121 ctggacttcc gaagtctcta tttgaggaga atctgtgacc ggacctgctg atttcatcat
     3181 taccaacgca aaagtattta ccagtgacga gacccatccg cacgctgaag ctgttgctat
     3241 tcaaggcaat cgtattcttt ccgtgggcag caaccaggat gtgctaagtt tgcaaagtaa
     3301 gaataccagg gtgatcgatg gaaaccaaaa gactctgatg cccgggttta ttgattcgca
     3361 ctttcatctc ttatccggct gcgaaaccct cggcgatgcc caacttcaga cggtaaaaaa
     3421 taaagccgaa ttaagatccg cgttgcaatc tcacgccaac gaaaataaaa ccgcaacctg
     3481 gatttccggg atcgggacta aatataagat cgtgtctacg cgtcaggagc tcgacgagat
     3541 catatccgat cgtccggttt acattgaagc ctatgatggt cacacgggct gggccaatac
     3601 aaaagcgctg gaactggcag gaattctgga ggatggcaaa acgacgggtc caaacggcgt
     3661 gatcgtgaaa gacgattcag gggtggcaac gggagagtta cgcgagcctg acgcaatcaa
     3721 ccctgttgcc agcttgatcc caacgccaga tgcctaccgc aaaagagaac tgctcaaaag
     3781 cggcattcag cagatcaatg cagcaggtgt gaccagcatc cacaacatga acggaaacat
     3841 ggaagattta atgatttacg ccgcattgga ggatgcagga gagatttcac tgcgggtgta
     3901 tgttccttat tcagttagac ctcatactac ggaggcgatg ctcgtcgaag cggtgaaaat
     3961 gtctgaggtg cagggagaat atgtccgcgg tggggccgtg aaattcttca tggatggagt
     4021 atgggagtct tatacggcgt tgaatataga accgtatgca gataacccga acgcgaactc
     4081 acctggcatt tactcggccg agcatttcac acgcatggga attgcctgcg acaagcttgg
     4141 tctgcaaatc tttgtccatt gctgtggcga cggtgcagta agacgaacgc tggatggata
     4201 tgaagcaatt caaaaaataa acggaaagcg cgacagccgt caccgagtcg agcatattga
     4261 agtgattcaa gaggacgatc taccacgttt caaggagtta ggagtcattg cggcgatgca
     4321 gccattacat gccccattaa ctatgaacgg cgatgtatgg ttggatcgaa caggaagctc
     4381 acgctggtct cgctcgtttg catggcgctc gatcaaggat gccggagcaa tacttgccct
     4441 gggtagtgac tggaccgtag cttccttcaa cccgatgctt ggcgtccatg cggcgctcaa
     4501 cagacagaaa tggtctcccg aagaccccga tcagcgattg acgctggaag aggttctgat
     4561 aggatatacg cgcgatgcgg cttttgcaga attccaggaa catcaaaaag gaatattaag
     4621 ggaaggatat ctcgcagatc tggtattgct ctcggaaaat atttttgaaa taccacacgc
     4681 ggaaatggca aacgtaaaac tcgtgctcac gatggcaaat ggaaaaattg tatacgaagc
     4741 ctgaaccgag gagcgaatca ttgtcttcca aagcagacct cgcaatcatt aacgcgcgca
     4801 tatttaccag cgatgaaacc attccttacg ctgaagcagt agccatcgaa ggcaagcaca
     4861 ttttgtatgt gggaaacaac actggcgctc aaaagttttg tgatgacaaa acgcgcgtaa
     4921 ttgatgggca aggtcatacg ctgaccgcgg gattcattga tacgcatgtc catttgttat
     4981 ctggcgctac ctggatgggt tatgcagaac tttccttggc ttctacaaag aatgacctaa
     5041 agggaatact gcgcgagtat gcagatgcaa atcaatctga ggaatggatc attggctggc
     5101 gcagcccata caacgttatc tcgaccaggg gcgaattaga tgagatcatc cctgaccgcc
     5161 ctgtgtacgt gcgggctgcc gatgcacaca ccgcctgggc aaacacgaag gccctcgaaa
     5221 tcgcaggcct tctgaaggga aacaggacgg atttgccaga tggaattgtg ctcgatgaaa
     5281 atggtcttcc aacaggtgag ttgcgcgaag ggaaggcgat gagagctgtc ctggatttca
     5341 tccctccgcc cagcgaggcc cgtattcgtg atcaactcaa acgcgccatc cagggattca
     5401 acagaaccgg aatcaccagc atccacaaca tgaacggcga tatgaatgat ctcatggtct
     5461 acgccgcggc tgaggatgcc ggtgaactga atctccgcgt gtacgtacct tattcggtca
     5521 ggcctgaaac tcgagaagaa gacttatcag aagcttcaaa aatggcagca gtccaaatgg
     5581 attatgttcg cggcggcctg gcaaagttct tcatggatgg cgtttgggaa tcctatacgg
     5641 cgttcaacat cgagccttat gccgataacc ctgagatcag cgttgacccc atcttttcgc
     5701 ttgagcactt tacacggatg gcagccgcct gcgacagact cggattgcaa atcgcggtgc
     5761 actgctgcgg cgatggtgca gtcaggcaaa ccctggacgg ttatgaagct ttacagaaaa
     5821 tgaatggaag gcgcgacagc cgccatcgca tcgagcatgt tgaggtttgt cagcctgaag
     5881 atatgccacg tttcagggat ttgggtgtta ttgcagctat acaacccagt catgcgtcgc
     5941 taaagttgga cgattacaaa atcgggcaag tccggctggg cgagaaacgc tggccactat
     6001 ggcaaccatg gcgcgacttg aaaaacgccg gagcacatat ctctctcggt agcgattgga
     6061 cggtggtgcc ttatgatcca atgacacatt tctacgtagc gctcaatcga gaaccatact
     6121 cacccgacag cacagaccag cggcttaccc ttgcagagtg catcctcggt tatacgcggg
     6181 acgcggcgta tgcggaattc aaggaaaatg aaaaggggca ggtgaaggaa ggctatttg
//
LOCUS       DASTJP010000049         4157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_781882, whole genome shotgun sequence.
ACCESSION   DASTJP010000049 DASTJP010000000
VERSION     DASTJP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781882"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VFQ23_01465"
     CDS             <1..1227
                     /locus_tag="VFQ23_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HET9905268.1"
                     /translation="NRVMIFDFGLSKTIFTLIAIFPYLFSPIQVAIGSFADHHPIFGY
                     RRTPHIFLGLLLCATGLGLSSFMVLTIQTNFTLGILVGLLAFFLWGMGYHFSAVSYFS
                     LASELSGEKGRGTTIAIMFFVMVFGIIAASQTVSRLATEPTFESLSRAFWIVSISALT
                     LGMLSLIKLEPRFNASSSNQASKTHTLREMYSAITANPVARVFFIYLLLLLAAILGQD
                     VLLEPFGAQAFGMTIAETSRIVSYSQLLTLIAFFVAGFMEGRVEKKKIAQMGNFGAIL
                     GFVLIVISGMTRSLGLFYAGIMLLGFGTGIATVANLSLMYNLTVHEEVGLYIGAWGFS
                     NGLSRLTGLLMAGVVADLATRITGNAISGYLVVFGIEAFMLFGAAVMLSRIDVDRFKK
                     QAQEPSYLDKVALAAE"
     gene            1232..1501
                     /locus_tag="VFQ23_01470"
     CDS             1232..1501
                     /locus_tag="VFQ23_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905269.1"
                     /translation="MRANGLAYLAGSNLNPSSQEDQEVAEVCIPNISPKERKKREQFA
                     MSFFLFTLLVLVGLLAFDVNPLWRLPLFFMFSAATTSYIQSLDKT"
     gene            1669..2265
                     /locus_tag="VFQ23_01475"
     CDS             1669..2265
                     /locus_tag="VFQ23_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9905270.1"
                     /translation="MTDEWLSLKGAAELLGVHPSTVRLWSNKGHLPVHLTQGGHRRYL
                     RHEVELWKANRQQVEPGEMMQMVIRNVRVQIADGKLEAEAWYQKLNANARMQYRESAR
                     SLFHGLMAFISDGEKAAVESHAIGYEYASRARRYGLSYVDAARAFLFFRYALIESVVG
                     VYRKANVPSQDILYKIHSFTDEILISLLDTYKKLESGK"
     gene            complement(2294..3112)
                     /locus_tag="VFQ23_01480"
     CDS             complement(2294..3112)
                     /locus_tag="VFQ23_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905271.1"
                     /translation="MPINQALPFFSTAIMLGFTIYVLQRFFIRRQLHFLFWGVGLAMF
                     GAGSFAEAYLALAWNKWVFFAWYLFGAALNAAWLGHGTLYLLVRKKWVHAITVLLILG
                     SLFAGYLMLQVMPNLDTDVFTTEKLISEQYGTKVLGDNETAPADDQTTQTIYRGQNVT
                     VVRGLLPLGSAVRLTTPFFNIYGLLTLVGGAIWSAYLFWRKRVMPNRVIGNVLIAAGA
                     LLIGFASTLTRLGYGQFLYLGELLCAILMFAGFRFAARPQPDEVPQSIPVAATD"
     gene            3450..3938
                     /locus_tag="VFQ23_01485"
     CDS             3450..3938
                     /locus_tag="VFQ23_01485"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HET9905272.1"
                     /translation="MILDFPPFLATRRNHALEHATLHILAQKYRDQNMAGHSNPTGFF
                     LFGDMTTEDIRNAIHEAMTRLHAGERGLAIHPGCGTNLATTTLLPATFAWVPLQSTRS
                     LRWRLLLIPIALMFAAFGYLLSKPLGPWLQANVTTEADLGSMQVTDIVPVRQGVHRVI
                     TK"
BASE COUNT         1018 a          986 c         1026 g         1127 t
ORIGIN      
        1 aaccgcgtca tgatctttga ctttggtctt tcgaaaacaa tcttcacctt gatcgctatt
       61 ttcccttatt tgttttcgcc gattcaagtt gccattggtt cctttgccga ccatcatcca
      121 atctttgggt accggcgaac acctcatatt ttcctgggct tgctgttgtg tgcaaccggg
      181 ttaggtctat cgtcctttat ggtcttgacc atccaaacaa actttaccct cggaatcctg
      241 gttggcctgc tggctttctt tctgtggggc atgggatacc atttctcggc ggtttcctat
      301 ttttcactcg cctctgagct ttcaggtgaa aaaggtcgcg gcacgacgat cgccatcatg
      361 ttcttcgtca tggtctttgg aattattgct gcatcgcaga ccgtcagtcg tttggcgacg
      421 gaaccaacct ttgaatcctt atccagggca ttttggattg tttccatttc tgccctgacc
      481 ctgggtatgc tcagcctcat caagcttgag ccgcgcttca atgcttcctc ctcaaaccag
      541 gcatccaaaa cacatacctt acgagaaatg tattcggcta tcaccgccaa cccggtggca
      601 cgcgtgttct ttatttatct gctgctatta ctggctgcca tccttgggca ggatgttctg
      661 cttgaaccat ttggtgcaca ggcctttggt atgaccatcg cggaaacctc acgtattgtt
      721 tcttacagtc agttattgac tctcatcgcc tttttcgtgg caggtttcat ggaggggcgg
      781 gtggagaaaa agaaaattgc tcaaatggga aatttcggtg caattttggg attcgtactc
      841 atcgtgatca gcggaatgac acgcagcctg ggcctctttt atgctggtat tatgttatta
      901 gggtttggaa cgggtatcgc gacggtcgca aatttatcct tgatgtacaa cctgactgtg
      961 catgaagagg tgggcttata cattggtgcc tggggtttct cgaacggact ttcgcggcta
     1021 acagggctat tgatggcggg tgttgtcgca gatctcgcca cacggatcac tggaaacgcc
     1081 ataagtggct accttgtggt ctttggcatc gaggcattca tgctgtttgg tgccgcagtg
     1141 atgctttcca gaattgatgt agatcgattt aagaaacagg cgcaggaacc atcatacctg
     1201 gataaggtcg cacttgccgc ggaataaagt catgcgtgcg aatggtttag cgtacctggc
     1261 cgggtcgaat ctcaatccat cttctcaaga agatcaggaa gtcgctgaag tttgtatccc
     1321 caatatcagc ccaaaggaga ggaaaaagcg tgagcagttt gcgatgtcat tcttcctctt
     1381 tacgcttctg gtcctggtgg ggcttctcgc cttcgatgtg aatccgttgt ggcggttgcc
     1441 gttgtttttc atgttcagcg cggcgaccac aagttatatt cagtcgcttg ataagacctg
     1501 agtatcactt gcgtcgcgcg gtctgcgcaa cacctctgac aagactgaaa gaatcgaaga
     1561 ccctgaagaa ttgcgacagg ttcagcgtca ggcactgcgt gtgatattca aaggaattct
     1621 tgtcgctctt ccactaaccg tgatcgcata tctactccct caataaaaat gaccgatgaa
     1681 tggctttccc tgaaaggtgc ggcagaacta ctaggtgtgc atcccagtac ggtcaggctg
     1741 tggtcgaaca agggacacct gcctgtgcat ctcacgcagg gtggtcatcg ccggtatctt
     1801 cggcatgaag tggaattatg gaaggcaaac cgtcaacagg tggagcctgg ggaaatgatg
     1861 cagatggtca tccgcaatgt gcgggtgcaa atcgccgacg gaaaactcga agccgaggca
     1921 tggtatcaaa aattgaatgc caatgcccgc atgcagtatc gagagagcgc gcgttcgttg
     1981 ttccatggat tgatggcttt tatatctgat ggcgaaaagg cggctgttga atcccatgcg
     2041 attggatatg aatatgcctc acgcgcccgt cgctatgggt taagttatgt ggacgcagcg
     2101 cgtgcatttt tattttttcg ttatgctttg attgaatcag tcgttggtgt gtatcgtaag
     2161 gcgaatgtcc cttcgcagga tatactctat aagatacaca gctttaccga tgaaatattg
     2221 atcagcttgc ttgatacata taagaaactg gaatcgggca aataaagaga gattcttttt
     2281 ctgtctccag gcactaatcg gtagcagcta caggaataga ttgaggaacc tcatctggtt
     2341 gaggacgggc cgcaaatcgg aagcctgcaa acatcaggat ggcgcagagt aattcaccca
     2401 aatataggaa ttgaccataa cccaggcggg tcaaggtgct ggcaaagcca attaaaagcg
     2461 cgccagctgc aatcagcacg ttgccaatca ctcggttggg cattacacgt ttgcgccaga
     2521 acaggtatgc cgaccagatc gctccaccga ccagtgtgag caagccataa atattgaaga
     2581 acggagtggt gaggcgcacg gctgaaccaa gtggtagaag accgcgcacg acagtcacat
     2641 tctggccgcg ataaatggtc tgggtcgttt ggtcatctgc tggagccgtc tcattatctc
     2701 caagaacctt cgttccatat tgttcgctaa ttagtttctc ggttgtgaaa acatccgtat
     2761 ccaagttggg catgacttgc agcatgaggt agccagcaaa caaactgccc aaaatcaata
     2821 gcacagttat agcgtgtacc cactttttgc gcacaagcag gtagagtgtt ccatgtccga
     2881 gccaggcagc gtttaatgcc gcgccaaaaa gataccaggc aaagaatacc cacttgttcc
     2941 acgccagcgc cagatatgcc tctgcaaaac tgcccgcgcc gaacattgcc aacccaacac
     3001 cccaaaacag aaaatgtagc tggcggcgga tgaaaaagcg ttgcagaacg tagatggtaa
     3061 accccaacat gatcgcggtt gaaaagaacg gtagagcctg attaatgggc ataatggtta
     3121 tcctccagtc gaaattagaa atggacaatg ccggtcagta cgccaacagc gaaaagaatt
     3181 agacaaaggg taataattcc cattagaagc aacaaggatg ggacgatctt ttcgaaaaat
     3241 aacgagtttc gagggatgtg tgattttttg attttgtgat catggtcggg catgtttacc
     3301 tttgttgata gcgagccttg actcccaatt tcatgatgcc tattatctgt cttcaagcat
     3361 agtggtcaat gtaggtttgt ataaatttcg attagaggat tcttatttgt caatgttcgt
     3421 aagtatggct ttaacgctat aataaaatca tgatattaga tttccctccc ttccttgcta
     3481 cgcgacgtaa tcacgcgctt gaacacgcaa cactccacat tttggctcag aaatatagag
     3541 atcaaaatat ggccgggcat tccaacccga cgggattctt tctgtttggt gatatgacga
     3601 cagaagatat ccgcaatgcg attcatgaag ccatgactcg tttacacgcg ggtgaacgtg
     3661 gactcgcgat ccatcctggc tgtggcacaa accttgcaac caccacactt ctccctgcga
     3721 cgtttgcctg ggttccgctt cagagcacgc gttccctacg ctggcggctc ctgttgattc
     3781 caattgcttt gatgtttgct gcatttggtt atttactcag caaaccactg ggtccctggc
     3841 tgcaggcaaa tgttacgacc gaggcagatc tgggttcgat gcaagtgaca gatattgtcc
     3901 cggttcgtca aggcgtccat cgcgttatta cgaaataaat ttgtagggat gggcagcgcc
     3961 catccctact ctattacttt tatgcccacg attcacttct tcttcggcaa tgatgaattc
     4021 gccatttctc gaaagttgaa ggaatttgaa tcggacttta ccgatcctac cagtgcagac
     4081 atgaacaccg cgcgtcttga tgcgcgttcg atcagcgaag atgatttggg caacgcggtt
     4141 aatgccatgc cgttcct
//
LOCUS       DASTJP010000050         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_450468, whole genome shotgun sequence.
ACCESSION   DASTJP010000050 DASTJP010000000
VERSION     DASTJP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450468"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFQ23_01490"
     CDS             complement(<1..590)
                     /locus_tag="VFQ23_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9905273.1"
                     /translation="MNTIIDQTLQVTHVSKRYGSGTTAVTAVHDVSLSVSPGEIVLIM
                     GPSGSGKTTLLSMLGALLKPSEGTIQLNGTVISALAESQLPEIRLNQFGFIFQDFNLL
                     SALTTLENVAIVAELAGSRSSTAGRKATDLLKELGLGERLNFLPEKLSGGEKQRVAIA
                     RALVNDPALILADEPTANLDSKIGQEIMRLLRRIAK"
     gene            complement(596..1708)
                     /locus_tag="VFQ23_01495"
     CDS             complement(596..1708)
                     /locus_tag="VFQ23_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905274.1"
                     /translation="MTRLAFRNLFQNKVRLMISTGGVALALLLILALDAIFTGVEQRI
                     TAYIDYSGADIWVSQSDVFNMHMASSSLPDSVARKVRLVPGVATVSPILYVANNVVVG
                     EERNLAYIIGVAERAQLGGPWNVSRGLNIPGEGQAIMDLSVAEKSGVELGDLVEILGE
                     ELEVVGLTEGTSSLVNSIAFISQEDFETARGSYDTFSFLLVKVREDESAQAVANRIRV
                     RVRDVTVQTREQFSGQERQVVSDMSTDVVTIMNLIGFMIGLAVMALTVYTSTLSRRHE
                     YGMLKALGARNTDVYFTVLAQAMLSTLLGFFFGWAITWLLTLTIPFTGLALTVVINPN
                     SLIKVAVVSLLLAGLSAMIPIRQIANLDPAMVFRGT"
     gene            complement(1705..>2396)
                     /locus_tag="VFQ23_01500"
     CDS             complement(1705..>2396)
                     /locus_tag="VFQ23_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9905275.1"
                     /translation="DRVLADRHGLRIGDQVEVMGKDFTIVGLSSGTTSWMTSFFFIRK
                     KDAENLLLAPGATSFLLLTTTTNSDTERILQRLHDFSGVNALTKQTMAANDLKLFARI
                     FSAPLKLMVGISFLVGTMIVGLIIYTATVERQREYGVIKAIGAKNRFLYQIVLTQALF
                     ASITGSSLGILLANGAAQWIMAARPQFLIIFDSGDSAQALLAGLGMALLAAIFPTRVV
                     ANLAPAEVFRK"
BASE COUNT          621 a          623 c          598 g          554 t
ORIGIN      
        1 tctttagcga tacggcgcaa gagacgcata atttcctggc caatcttaga atccagattt
       61 gccgtcggtt cgtccgccag gatgagggcc ggatcgttga caagggcgcg tgcaatcgca
      121 acacgctgtt tttcaccacc agataacttc tccggaagaa agttcaaacg ttcaccaaga
      181 ccaagttcct tcagcaagtc tgtggctttc cgtcctgccg tactggaccg acttcctgca
      241 agctccgcga cgatggcaac gttctcgagg gttgtgagtg ccgataacag attgaaatcc
      301 tgaaatataa aaccaaattg attcagacgg atctcaggca gttggctttc tgccagggca
      361 ctgatgaccg tcccatttaa ttggattgtt ccttctgatg gctttaatag ggcacccagc
      421 atggaaagca gagtagtctt accggagccg gatgggccca taatcagtac gatctcgccc
      481 gggctcaccg ataaggagac atcgtgaacc gctgtcactg ctgttgtgcc tgatccgtaa
      541 cgcttgctta cgtgagtaac ctggagtgtt tggtcaatga ttgtgttcat tcgctttacg
      601 tacctcgaaa taccattgcc ggatccagat tggcaatctg cctaatagga atcatggccg
      661 agagtcctgc caggagaaga gaaacaactg ctaccttaat gagtgagttg ggatttatta
      721 ccactgtaag agccaaaccg gtgaatggga tcgtaagcgt gagcaaccag gtgatcgccc
      781 agccaaagaa aaatcctaac agggtggaga gcatcgcctg tgccagaact gtgaaataca
      841 cgtctgtgtt gcgcgcgccc agtgccttca acatgccata ttcatggcga cgcgagagcg
      901 tggatgtata cacggtaagc gccattactg ccagcccgat catgaagcct atcaggttca
      961 tgatggtgac gacatccgtg ctcatgtcac tgacgacttg tcgttcctgt ccagaaaact
     1021 gttccctggt ctggacggtc acatctcgga cgcggactcg aatacgattc gccactgctt
     1081 gtgctgactc atcttcgcga acctttacca acaagaagct aaacgtgtcg tagctcccgc
     1141 gcgcagtttc gaaatcttcc tgagaaatga atgcaatgga attcactaga ctggaagtac
     1201 cttctgtgag acccacgact tccagttcct ccccgagtat ttccaccaaa tcacccagct
     1261 ccacacccga tttttccgcc acactcagat ccatgattgc ctgtccttcc ccaggtatgt
     1321 taagccctct ggaaacattc catggacctc caagttgagc tcgttcggct acgccgataa
     1381 tgtatgcaag attccgttcc tcgccaacaa ccacgttatt tgcaacgtac agaatcggag
     1441 agaccgtggc tacaccgggg acaagcctta cttttctggc aacggagtcg ggcaatgaag
     1501 aagaagccat atgcatgttg aatacgtccg actgcgacac ccagatatcg gcgccgctgt
     1561 agtcgatgta cgctgtaatg cgctgctcaa cacccgtgaa gatggcatcc aaagctagaa
     1621 tgagcaaaag agcaagtgcc actccaccgg tggagatcat cagccgcact ttgttctgga
     1681 aaagatttct aaaggcgagc cgagtcattt acgaaagacc tccgctggtg ccaggtttgc
     1741 aacaacacgc gtggggaaga tagccgcaag gagtgccatg cccagacctg caaggagagc
     1801 ttgcgcgcta tccccagaat cgaagatgat caggaattgc ggccgagcag ccatgatcca
     1861 ctgtgcagcg ccattagcca gcaggatgcc gagagaggat ccggtgatcg aggcaaacag
     1921 ggcttgggtc agcacaattt ggtagaggaa gcggttcttt gcgccaatcg ccttaatgac
     1981 gccatattct cgctgacgtt cgaccgttgc cgtataaatg atgagtccta cgatcatcgt
     2041 tccgactaaa aacgagatac cgaccataag ctttaacggg gccgaaaaga tcctggcaaa
     2101 taatttgagg tcattggcag ccattgtttg cttggtaaga gcgttgacac ctgagaaatc
     2161 atgcaggcgc tgcaaaatcc gctctgtatc ggaattcgta gtcgttgtga gcaggagaaa
     2221 gcttgttgca cctggagcga gtaacaaatt ctcagcatct tttttgcgaa tgaagaagaa
     2281 actagtcatc caggatgtag tgccgctaga cagaccaaca atggtgaaat cttttcccat
     2341 gacttctact tgatcaccga tacgcaaacc atgccgatct gcaagcacgc ggtcga
//
LOCUS       DASTJP010000051         7631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1415956, whole genome shotgun sequence.
ACCESSION   DASTJP010000051 DASTJP010000000
VERSION     DASTJP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415956"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1572)
                     /locus_tag="VFQ23_01505"
     CDS             complement(91..1572)
                     /locus_tag="VFQ23_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905276.1"
                     /translation="MSKPDLILLALEDSDSAILRLMDSVLHAARYETAIAKDTAALSR
                     ILQEASPALVLVGEKFGGHDSLKISKELLERFPTLPILIYSQKPTVELTKGIFRLGLS
                     GYISPPLGTQDIVDAVENSLRNAHRVGDWLRHEVKRTTASLEKRAQISEAERLRLEAV
                     FNNIHDSVMILSKENRILLVNPAMCRAFGLDPKAVVGKPVLEAINHPDLQALITPTHD
                     DDPLKYYEVSFPDGRVGNARIASIHEVGYAITMQDITYLKEIDRIRSEFVHTVSHDLR
                     SPLTSVIGYAELVERAGSLNENQQDFLHRIQDSVQHITELINDLLDLGSIEAGMDTRR
                     EYVQLEGILHYTLEMLQGQIKSKSIKVQTDIGLALPALRANPIRLRQVLDNIVGNAIK
                     YSATNGEVKISIHPEGDQLILQVTDEGPGIPAKDQPHVFDKFYRASNVNSNVDGSGLG
                     LAIVKTIVENHQGRIWLESTLGKGSSFFVVLPIHSEAAQVIKK"
     gene            complement(1588..2760)
                     /locus_tag="VFQ23_01510"
     CDS             complement(1588..2760)
                     /locus_tag="VFQ23_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9905277.1"
                     /translation="MASTGDRILLVENDPEISDIIARQALKSVGYQVDVVQDSSSAIK
                     RSLESPPDLIIADLNLPGLSAKDLLVALTSQGVTTPLLVIASKGQEQDIIQAFRLGAA
                     DYLLWPARDAEVVSVVERVFQRVHEIRDRQRLDLKLSETNQELQRTVRELTAIINIGK
                     AVISITDQKQLFQKIVDGAVQVSEGDISWLLVKSEQRGTFLLTAHRGLPETWSKKMNM
                     ALEDGISGLVAVSGETLRITGDALLKFRVANLGKSACAVPIKVQKEVIGMLVVVRKEE
                     RQFETLEQTLLEAVADYASISLVNARLFRALHESMQASKYGEKQQNALLESIRSSIME
                     ELQSATYPVDLLLTEKQGQLSNYQRQALQTARAALQRLARAAEKTTPPVPIKLKKQ"
     gene            2963..5161
                     /locus_tag="VFQ23_01515"
     CDS             2963..5161
                     /locus_tag="VFQ23_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET9905278.1"
                     /translation="MSLPFDFRKLLPVASIGLVGGVILLPLTISFALLMFSAEDLSPF
                     ASTGIGLVLFGAFIMQLIIALMSSFPGMMGGPQDSPAAILSLTALVIATHMQGASAEA
                     KFITVVMTIILTSVVSGLFFVMIGTFQLSRLVRFIPYPVVGGFVAGTGLLLTQGALGV
                     MLGKAPGIADLGVLLKAETLFLWVPGFLFGAIVLVASRRSTHYLTYPVLLIGAVLFFY
                     LFIWLGGYDLQQARQMGWLLGPFPQGTLWRPLDLSLLRQVDWAVIAGQSTNIFAVSVI
                     SLVGLLLNASALELIAQKDVDLNRELISTGIANLAGGLAGGSVGYHYLGVSALAFRMG
                     ISSRLVAFFGASVTGLALLFDASLLSLIPKLVAGGLILFVGLSFLVEWLYDAWFQLPR
                     IDYVLVWVILIVVGAVGFLEGVGTGIAIAIILFVVNYSRIGIVKDTLTGKSYQSNVER
                     PLEQRQIIKEAGEHILILRLQGFIFFGTSQSLVSQVNARVKDPTRVKLRFLILDFQHV
                     SALDASALFGFVRLKQMATGNQFHLIFSETNKDIKDRLARNGFEESEELIRFFPTMDY
                     GMEWCESRLLVDEGSSSIIRAGSLKGQLQKLLPSSEYVEKFMTYVERREARQYHILIN
                     QGDPPDCMFFIDSGEVTTRLEMSKGKFIRLKSQRGGTMVGEMGLFLKQPRTATVVVSE
                     PSVLYRLSVDAYERMMQDDPDLVFALHQWITRVLATRLTENNSTLEVLLN"
     gene            complement(5158..6255)
                     /locus_tag="VFQ23_01520"
     CDS             complement(5158..6255)
                     /locus_tag="VFQ23_01520"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET9905279.1"
                     /translation="MTDNHPNPFPKGEGTAEPLFEVTRGRIVESVHYGSIAVVDSNGN
                     LISSYGDPKAVAFLRSSAKPFQVIPFVEHGGVEHYGYTGRELSIACASHNGSDLHVQT
                     IEEMQKKIGLDEGHLQCGTHMPSDVDAFKTLVKNNQQPSPNRNNCSGKHTTMLAYAKM
                     CGHSLDNYLDINHPIQQDILATFSDLCLTPPQEIERGIDGCSAPNFAVPLYKAALGFA
                     RLCDPHVLSEPRAAACRKITSAMTAYPEMVCGYGELDEQLMRVGEGKLVSKGGAEGYQ
                     IIGLLPGVLRPDSPGIGIALKVSDGDASSMESNLESIHRVRPAVALEILRQLGAISVK
                     QEQALAGFGPQRPVTNLRGLVVGQSRPVFEL"
     gene            complement(6255..7115)
                     /locus_tag="VFQ23_01525"
     CDS             complement(6255..7115)
                     /locus_tag="VFQ23_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9905280.1"
                     /translation="MEELAKNVYIEDKYMGVTVAVISQPRGLVQIDAPPSPEDGRSWR
                     ASLMGMGNGPERVLINLDAHPDRTLGVRAMDCTVIAHEKTAQAFRNRPNTFKAQGDET
                     GSNWEGIPGLGSVRWAPPEISFVDQMSLYWSDTPILLESHPGPTSGAIWVILPDEKII
                     FIGDAVVKGQPPFLAYADLPAWIETLNGLMDPKYKGYTIVSSRSGIVNQQTIRQQLVL
                     LKKIHDRLEKVGKKKPVLGVTDKLADQILKDFRAPAERKKQFLHRLRYGLNHYYMRHY
                     HPSNRHTLDE"
     gene            complement(7119..>7631)
                     /locus_tag="VFQ23_01530"
     CDS             complement(7119..>7631)
                     /locus_tag="VFQ23_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905281.1"
                     /translation="PVADQKVVDPLLLIKNAIRDVEPVAKGRRQTFILDLPEEMPSIW
                     VDEDMARRVLINLMENASKFTLADELLGVGARLEGDWVHMWVKDNGRGIPAAEQERIF
                     DKFTRLRGTSKVGGLGIGLAFCRLAVLGHGGRIWVESEQDRGATFHFTFPVATEAQLA
                     SSPDKKTGEH"
BASE COUNT         1817 a         1920 c         1848 g         2046 t
ORIGIN      
        1 ctgcgggaca aaagcgacag aaaagctcaa tcctttcgca attgccgaaa aaatgaatta
       61 tcttagagta ttaaagcaga tcagaggata ctacttcttt ataacctggg cagcttcaga
      121 gtggatcggt agaacaacaa agaaagaact gccttttccc aatgtagatt ccaaccaaat
      181 gcgcccctga tgattttcca caatggtctt cacaatagcc aaccccaggc ctgagccgtc
      241 cacgtttgaa ttgacgttac tggctcgata aaatttatcg aatacatggg gctggtcttt
      301 tgctgggatt ccgggtccct cgtcggtgac ctgcaggata agctgatctc cttcagggtg
      361 aattgaaatc ttcacttctc cgtttgtcgc tgaatacttg attgcattcc caacaatatt
      421 atccagaacc tgtcggagcc gaatcgggtt ggcacgcagg gccggtaagg ctaagccaat
      481 atcggtttga accttaatgc ttttcgattt tatctgtccc tgcaacattt ccagcgtata
      541 gtgcagaatg ccttccaatt gaacgtattc gcgccgtgta tccatccccg cttcaatact
      601 acccagatcc aacaggtcgt tgattagctc agtaatatgt tgaacactat cctgaatacg
      661 gtgaagaaaa tcctgctgat tttcattgag ggaaccagct cgttcaacaa gttctgcata
      721 tccaatgacc gacgtaagcg gtgagcgtaa gtcgtgtgaa accgtgtgga caaactcgct
      781 gcggatgcgg tcgatctcct tcaggtacgt gatatcctgc attgtgatgg catatcccac
      841 ttcatgaatc gatgcgattc ttgcattgcc aactcggcca tccggaaaac tgacctcata
      901 atactttaat ggatcatcat cgtgtgttgg cgtgatcaac gcttgaagat cggggtgatt
      961 aatagcctcc agcactggct tgccaacaac cgctttcgga tccagaccga aagcgcgaca
     1021 catggcggga ttcaccaaaa gaatcctatt ctccttactc aggatcatca cgctatcatg
     1081 tatattattg aagaccgctt ccaaccgcaa acgttcggct tcagagatct gagcacgttt
     1141 ctcgagcgaa gccgtcgtgc gttttacctc atgacggagc caatcgccga cccgatgcgc
     1201 attccggaga ctattttcaa cagcatcgac gatatcttgt gttccgaggg gaggggaaat
     1261 ataaccgctc aggccaagcc tgaaaatgcc ttttgttagt tcaaccgttg gcttttgtga
     1321 ataaatgaga atgggcaggg tgggaaaacg ctcaagcaat tccttggata ttttcaagct
     1381 atcgtgtccg ccaaactttt cgcctactaa aaccagtgcg ggactggctt cctgtaagat
     1441 tcgtgacagg gccgcggtat ctttggcgat tgctgtctcg tatctggcag cgtgcaaaac
     1501 gctatccatc aatctaagaa ttgcagaatc ggagtcttcc agcgcgagca ggattaaatc
     1561 aggcttcgac ataggggtga gaggatttta ctgctttttt agtttaatag gaacaggcgg
     1621 tgtcgtcttt tctgcagcac gcgccaatct ttgaagggca gcgcgcgctg tttgcagagc
     1681 ctgccgctga tagttagaaa gctgaccctg tttttcggtc aacaacaaat cgacaggata
     1741 tgttgcagat tgcagttcct ccataattga actgcgaatt gattctaaca gggcattttg
     1801 ctgcttctct ccatatttgg aggcttgcat cgattcatgc aaggcgcgaa acaatcttgc
     1861 gtttacgagg gaaatggaag cgtaatctgc tacggcttcc agcaacgtct gctccagcgt
     1921 ttcaaattgg cgttcctctt tgcgaaccac taccagcatc ccgatcactt ctttttgaac
     1981 cttgatcggc actgcgcagg cagattttcc caggttcgca actctgaatt tgagcagggc
     2041 atcacctgtg atccggagag tttctccgga aaccgcgacc aatccgctga tcccgtcttc
     2101 caatgccatg ttcatttttt tcgaccatgt ctcggggagt ccacgatgtg cagtaagtaa
     2161 gaacgtccct cgctgttcgc tttttacgag gagccaggaa atatcccctt ctgaaacctg
     2221 cactgcacca tccacaatct tttgaaacaa ttgcttctga tcggtaattg aaatgaccgc
     2281 tttcccaata ttgatgattg cagtcaactc gcgcaccgtt cgctgcagtt cctggttggt
     2341 ttcactcaat ttcaagtcga ggcgctggcg gtctcgaatt tcgtgcacac gctggaacac
     2401 gcgttcaaca actgaaacca cttctgcgtc gcgagctggc cacaggagat aatctgccgc
     2461 gccaagacga aaggcttgaa tgatatcctg ctcctgccct ttactggcaa tgacgagcaa
     2521 cggagtagtt acaccctgag aggtcagcgc gacgagcaga tcctttgcgc ttaaaccagg
     2581 cagattcaaa tcggcaatga tcagatcagg aggagattca agtgaacgtt tgatagcaga
     2641 ggatgaatcc tgaacgacat ctacttgata gccaacggat ttcaaggcct gacgagcaat
     2701 aatgtcacta atttcagggt cattttcgac aagcaaaatg cggtccccag tagaagccat
     2761 cgtctaaact ctcagcgtgg attgtaacac cctttaccat ctttcaactc agcctgaggt
     2821 attgttatga gatagtcatt aagttaacaa tcaagttttt gcatttgcag ctgctgcatg
     2881 ttgaaatgtg gttgagacga aagttcatct ttggtgttaa tttcttggaa tatgcttcaa
     2941 aattgaggtg aggttcgcag cgatgtcgct cccgttcgat ttccgtaaat tgcttcccgt
     3001 tgccagcata ggactggtgg gaggtgtcat tcttctcccg cttactattt cattcgccct
     3061 gctaatgttt tcagcggagg acctctcccc atttgccagc acaggtattg ggcttgtatt
     3121 gtttggcgct ttcatcatgc aattaattat tgccctcatg agttccttcc ctggcatgat
     3181 gggcggaccg caggatagcc ctgccgcgat attgagcctg acggcactcg ttatcgccac
     3241 gcacatgcag ggcgcttctg cggaagccaa gtttattaca gtcgtgatga caatcatcct
     3301 gacatcagtt gtttcgggac tgttttttgt aatgattggt acgttccaat tgagccggct
     3361 tgtgcgtttc attccatacc ctgtggtcgg cggctttgtc gcaggcaccg ggttgttgct
     3421 aacacagggt gcgctgggag tgatgcttgg caaagcaccc ggcattgccg atctgggtgt
     3481 attgctcaaa gctgagactt tatttttatg ggtacctggt tttctttttg gtgcaattgt
     3541 cctggtggct tcaaggcgtt ctacacatta tcttacctac ccggtgctgc tcattggcgc
     3601 tgttcttttc ttttatcttt tcatttggct tggtggttat gacctacaac aggctcgcca
     3661 aatgggctgg ctgctggggc cctttccgca gggcactcta tggagaccat tggacttatc
     3721 gcttctcaga caggtggact gggccgtgat tgccggtcaa tcgaccaaca tcttcgccgt
     3781 ctctgtgatc agcctggttg gcttgttgct gaatgccagt gccctggaat tgatcgctca
     3841 aaaggatgtc gacttgaatc gtgaactgat ctcgaccggt attgccaacc tggcaggtgg
     3901 gttggccgga ggctcagtcg gctaccacta tttgggcgtc tctgccctgg cattccgtat
     3961 gggaatttca agtcggcttg tggcgttctt cggcgcttct gtgacagggc tggcgctctt
     4021 atttgatgca tcgctgcttt cgctaatccc caaactggtg gcaggtggac tgatcctttt
     4081 cgttggacta tcgttcctgg tggaatggtt atatgacgca tggttccaat tgcccaggat
     4141 tgattatgtc ttagtttggg tgatactgat cgtggtgggt gcggttggct ttctggaagg
     4201 cgtgggcaca ggtatcgcta ttgccattat tctgttcgtc gtgaattaca gtcgaattgg
     4261 tattgtcaag gacacgttga ccggcaaatc atatcaaagc aatgtggaac gtccgcttga
     4321 acagcgacag attatcaagg aagcgggtga acacatcctg atcttacggc ttcagggatt
     4381 cattttcttt ggcacctcac aaagcctggt aagccaggtc aacgcccgtg tgaaagatcc
     4441 aactcgggtc aaactgagat tcttaatcct ggatttccag cacgtgagcg ccctggatgc
     4501 ttctgctctc ttcggctttg tacgcttgaa acaaatggca accgggaatc aattccattt
     4561 gatttttagc gaaacaaaca aagacatcaa agacaggctg gcacgcaacg gctttgagga
     4621 gagcgaagag ttgattcgtt ttttcccaac tatggattat gggatggaat ggtgtgaatc
     4681 aagactatta gtcgatgagg gaagttcttc tatcattcgg gccggcagcc taaaaggtca
     4741 attgcaaaaa ctccttcctt cctctgaata tgtggaaaaa tttatgacgt acgtggagcg
     4801 aagggaagcc cggcaatatc acatcctgat caaccaggga gatccgccag attgcatgtt
     4861 ttttatcgat tccggtgaag tgaccacacg attggaaatg tccaaaggta aatttatccg
     4921 gctcaagagt cagcgcggcg gtaccatggt gggtgaaatg ggattgttct taaaacaacc
     4981 gcgcacagcg actgtggtag tttccgaacc cagcgtgttg tatcgtctgt ctgtagatgc
     5041 ttatgaaagg atgatgcagg acgaccccga cctggtcttt gctctgcatc aatggatcac
     5101 ccgcgtcctt gctacacgac tcaccgagaa caattctacg ttggaagtgt tgctgaacta
     5161 aagctcaaat acaggtcggg attgacctac gacgagacca cgcagatttg tgaccggcct
     5221 ttgcggtccg aagccagcca gtgcttgctc ctgtttcaca gaaattgccc ccaactgacg
     5281 caggatttca agcgccacag ccgggcgaac gcgatgtatc gattcgagat ttgactccat
     5341 actagatgca tctccatcag acaccttgag cgcaatgccg attccaggcg aatcgggtct
     5401 caatacgcca ggcaaaagcc cgatgatttg atatccctcc gcgccacctt tgctgacaag
     5461 ctttccttct ccaactcgca ttaattgttc atccaactcg ccatatccgc acaccatttc
     5521 tgggtaagca gtcattgcag atgtaatctt gcgacaggcc gctgcgcgcg gctcagaaag
     5581 tacatgcgga tcacataaac gcgcgaatcc caacgctgcc ttgtacagcg ggaccgcgaa
     5641 attcggtgcc gagcaaccgt caatgccgcg ttcgatctct tgaggcggag tcaagcataa
     5701 atctgagaaa gtggcgagaa tatcctgttg aatgggatgg ttgatatcaa gataattatc
     5761 gagggaatgt ccgcacattt ttgcataggc caacatggtg gtatgtttcc ccgaacagtt
     5821 attgcgattc ggactgggtt gctgattatt cttcaccagg gtcttgaagg catcaacatc
     5881 gctgggcata tgggttccgc actgtaagtg accttcatcc aacccgattt tcttttgcat
     5941 ttcttcaatc gtttgaacgt gcaggtcact gccattatga gaagcacaag caattgaaag
     6001 ctctcgcccg gtatacccat aatgttccac acccccatgc tccacaaatg gaataacctg
     6061 aaatggcttg gcactggagc gtaaaaaagc gaccgccttc gggtcaccat aggatgaaat
     6121 caagttgcca ttggaatcga caaccgcaat tgaaccataa tggacggatt ctacaatccg
     6181 tccacgggtc acttcaaaca ggggttctgc tgttccctct ccctttggga aagggttagg
     6241 gtgattgtct gtcatcactc atccaatgta tgacgattgg aaggatgata gtgacgcatg
     6301 tagtaatgat tcaacccata gcggaggcga tgcaaaaatt gcttcttccg ttctgccggg
     6361 gctctgaaat cctttagaat ctggtcagca agtttatctg taacgccaag gacgggtttt
     6421 ttcttcccca ccttttccaa acggtcatgg atctttttga gcaaaacaag ctgctgccgg
     6481 atggtctgct ggttaacgat tccactgcgg ctgctgacaa tcgtatatcc tttgtatttg
     6541 ggatccatta agccattcaa ggtttcaatc catgctggca agtcagcata cgccaggaaa
     6601 ggaggctgcc ccttgaccac cgcgtcgccg atgaagatga tcttctcatc aggcaagata
     6661 acccagatcg ctcccgaagt tgggccggga tgactctcaa gaagaatggg tgtatcgctc
     6721 caatataatg acatctgatc aacgaatgaa atttccggag gtgcccaacg tacactgccc
     6781 aagccgggaa tcccctccca attggagcca gtctcatcac cctgtgcttt gaacgtgttt
     6841 gggcgattac ggaatgcctg cgcagttttc tcgtgtgcga tcaccgtaca atccatggcg
     6901 cggacgccta aggtacggtc tggatgcgca tccaggttga ttagcacgcg ttccggtccg
     6961 ttgcccatgc ccattaggga ggcacgccac gagcgtccgt cttctggcga aggcggcgcg
     7021 tcgatctgga ccaggccgcg cggctgacta atcactgcta ccgttacacc catatattta
     7081 tcttcgatat aaacgttctt tgcgagttct tccatcaact aatgctctcc tgttttttta
     7141 tctgggctac tggctaactg cgcttccgtt gctaccggaa aagtaaaatg aaaagtcgca
     7201 cctcgatcct gttcgctttc gacccaaatc ctgccaccat gtcccaacac cgccagtcgg
     7261 cagaaagcaa gcccaattcc caaaccgcct accttgctgg taccgcgcaa acgggtgaac
     7321 ttgtcaaaga tccgttcctg ttccgcagcg ggaatcccac ggccattgtc cttcacccac
     7381 atgtggaccc aatccccttc aagacgcgcg cccactccca gaagctcatc agcaagggtg
     7441 aatttgctgg cattctccat caggttaatt aacacgcggc gggccatatc ttcatccacc
     7501 caaatggatg gcatttcttc agggagatct aaaatgaacg tctgacgtct cccttttgca
     7561 accggctcca catcgcggat ggcattcttg atcaggagaa gtgggtcaac taccttttgg
     7621 tctgctacag g
//
LOCUS       DASTJP010000052        19032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_781490, whole genome shotgun sequence.
ACCESSION   DASTJP010000052 DASTJP010000000
VERSION     DASTJP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19032
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781490"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VFQ23_01535"
     CDS             complement(<1..829)
                     /locus_tag="VFQ23_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9905282.1"
                     /translation="MSFAWPTLLLMLFCVPLLILFYLRIQQRRQKIAAQFGSLGVTRN
                     TTGKAPGIRRHVPAMIFLTGITLLLISMARPQATVSVPRIEGTVILTFDVSGSMSADD
                     LQPTRMEAAKLAALDFVDKQPPSVLIGVVAFSDGGISVQAPTENREETISTIERLVPR
                     RGTSIGNGILVALNTIAVAAGDPPLLTTTSMSDGQAPTAAPSPQGWYTSAVVVLLTDG
                     ENNQSPDPIAATDLAADLGVRIYTVGIGSAAGATITVEGFTVHSQLNEPILQYIADTT
                     "
     gene            complement(876..1781)
                     /locus_tag="VFQ23_01540"
     CDS             complement(876..1781)
                     /locus_tag="VFQ23_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9905283.1"
                     /translation="MSMPTSAVTPEKILLRLDWNVIRRLDGLLQGDYRTLFYGFGVDF
                     ADLREYQPEDDIRYIDWNVTARMDTPYVRQYVEDREITAWFLLDLSPSVDFGAVQSQN
                     EKRTMLVDFVTVIARLLTRHGNRIGAIMFGDKVQHTVPARGGRLQVLRLINDLLRQPR
                     LSRAPLTNLKEMLEGALNTIKRRSLIFIISDFISEPGWEKPLSLLNQRHEVLAIRLWD
                     PREVELPDLGMVVMEDAETGEQLFVDTHDPKFRKRFFEVAQKREEALNSTFKRAGVDV
                     LSLSTDEDLVRAIVRFAKQRKQYRK"
     gene            complement(1750..2760)
                     /locus_tag="VFQ23_01545"
     CDS             complement(1750..2760)
                     /locus_tag="VFQ23_01545"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9905284.1"
                     /translation="MAQNKNPENRTPMEKVLYEVKKIIVGQDHLLERMVVALLARGHV
                     LVEGVPGLAKTMAIKTLAESIGGEFKRIQFTPDLVPADLIGTRIYNQKSGKFETSLGP
                     VFTNLLLADEINRAPAKVQSALLEVMQERQVTIGRETFKVPDPFLVLATQNPIETEGT
                     YALPEAQVDRFMLKVLVGYPNSTEEFVIVERMTGMLQAVQKVLTTDQLLALQKDVDRV
                     YVDPALMEYAVRMVTATRKPQDVGLKDLQRFIMFGASPRASINLILTGRALAFVRGRN
                     YALPQDILDMALDVMRHRVVLSYEALSENVTGDHILERILDRIPIPVVPLHEHANVGS
                     NA"
     gene            complement(2832..3665)
                     /locus_tag="VFQ23_01550"
     CDS             complement(2832..3665)
                     /locus_tag="VFQ23_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9905285.1"
                     /translation="MNESSVSTDKPQSRLGSLRERVKGMFPFLSGVVAAFLAFFLYNF
                     LFPASQVTEAEVSNAIAGALASATPRAAYSVQVYQAIQPSLVMIESEFGGEGTVTDRG
                     LGTGVIVDDTGSILTSLHVVDDATRIVVTFADGTRAEAAIVNEQPESDIAALRTSTMP
                     EVWFPATLGNPGSMLVGDEAYVVGNPFGLYSSMSEGIISGFNRTFQPPNGKQPIEGLI
                     QFDAAANPGNSGGPLLNRNGHVIGIVTGIINPTADSFFVGIGFAVPIGTAAGGLGSPP
                     Y"
     gene            complement(3933..4370)
                     /gene="aroQ"
                     /locus_tag="VFQ23_01555"
     CDS             complement(3933..4370)
                     /gene="aroQ"
                     /locus_tag="VFQ23_01555"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET9905286.1"
                     /translation="MKVLILHGPNLNLLGTREPEVYGSMTLDDINNKLIELGNELGAE
                     VKCSQSNHEGALIDALHDARTWANGVIFNPGGYTHTSVALRDAISAIGIPVIEVHISN
                     VYAREEFRHTSLISAVCKGKVLGLGWRSYTLGLRALVEIAANP"
     gene            complement(4550..6079)
                     /gene="aroB"
                     /locus_tag="VFQ23_01560"
     CDS             complement(4550..6079)
                     /gene="aroB"
                     /locus_tag="VFQ23_01560"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET9905287.1"
                     /translation="MPHIFLYGPPGTGKSTVGRILSRNLNLPFVDLDRVVESNAGRSI
                     PQIMGQQGEESFRELETSALKKVLKQSESVIALGGGALLRNENRNLVETHGKVILLMA
                     ELETLFKRLNESSRQRPLLAGGLLEKLTSLLDVRKGHYHSFPMRLVVDQGTAEENAKR
                     AQIVLGRFHLRAMGEYDVIVESVEQVASLLSARDLKNPLIVTDENLTFYAEKIVASLS
                     KSGSAPSILTVPAGEEFKNLNTVSFLWKSFLAQGLDRRSTVIALGGGVIGDMAGFAAS
                     TYMRGISWVGIPTTLLSMVDASLGGKTGIDLPEGKNLVGSFYPPKLVLADPQLLITLP
                     EREFISGLAEVIKHGIISDPELFNLCARGLDFVKENLEQIVKRAMAVKIKIIEEDPYE
                     KGFRAALNLGHTVGHAVELASKFQLRHGEAVAIGLVTEARYAERIGVASKGLTDKIVE
                     VLSTIGLPTQISKELSRDEIVRAMKVDKKKDARVIRFALPVEIGKVELVEVTDLEKVL
                     D"
     gene            complement(6218..7411)
                     /gene="aroC"
                     /locus_tag="VFQ23_01565"
     CDS             complement(6218..7411)
                     /gene="aroC"
                     /locus_tag="VFQ23_01565"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET9905288.1"
                     /translation="MLRFLTAGESHGPSLTAILDGMPAGITLDPSIIDPELARRQKGY
                     GSGGRMKIEKDSVQILGGVMAGETTGAPIALLVQNADHVKWKGKAIEPMTAPRPGHAD
                     LTGAVKYGYKDLRPALERASARETTMRVAVGAVCKHFLSQFGIIVGGYVSSIGEIQAD
                     FGQMPYEERFARAEQSDVRCPIGTSAYQMREAIERTIHGKNTLGGVLEIVALNLPVGL
                     GSFMQWDKRLEAKLALAIMSVQAIKGVEVGDAFANARLPGTQAHDPIGLSPLSEGEGA
                     GVRAIHRLTNRAGGTEGGISNGQPIVIRAAMKPIATTLTPQQTVDLAKGQESPTNYER
                     SDFCPVPRAVPILEAMVAFVLADALIEKLGGDSLDEMRPRFESLRKARLEDLQMDNVP
                     HVFWE"
     gene            complement(7417..8271)
                     /gene="aroE"
                     /locus_tag="VFQ23_01570"
     CDS             complement(7417..8271)
                     /gene="aroE"
                     /locus_tag="VFQ23_01570"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET9905289.1"
                     /translation="MPLFNLGLIGYPLSHSLSPNIHSAALRACGFEGDYSLFPIYPED
                     KNGLQDLLSRVRSGEIHGLNVTIPHKQNVISFLDVLTSTSEAIGAVNTIYLREGKLIG
                     DNTDAAGFLTDLKNFLATAEQSNESKSALILGAGGSARAVVYALLNDGWDVIIAARRI
                     EQAQHLADSIANTKSPDVVSSFTDVEFSKIALIVNTTPVGMTPNIDQSPLPENLSLPR
                     HVSMYDLVYNPRETKLVKEARARGLNATTGLGMLIEQAALAFELWTGHNPSREIMRAS
                     VEGSPVSN"
     gene            complement(8281..9633)
                     /gene="aroA"
                     /locus_tag="VFQ23_01575"
     CDS             complement(8281..9633)
                     /gene="aroA"
                     /locus_tag="VFQ23_01575"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET9905290.1"
                     /translation="MLATLPSIQIKSIEYPLQATVRVPGSKSLTNRALLISSLANGKT
                     RLTNALFSDDSSYFARALQTLGFDIELDEHNHKMTITGQGGIIPANKAELFIGNAGTA
                     ARFLSAFLTLGNGEYVLDGDARMRERPIGDLVTALTQLGAQLSPLSLMERVDGERVAA
                     SICPPIKIIASGLPGGKTKIAGNISSQFLSALLMIAPYANSPIEIEVTTDLNSKPYVD
                     LTIAMMRDFGVEVERREYSQFTIHPNSYSPLSTYNIESDASAASYFFAAPAICGGKIR
                     VENISRKSKQGDINFVDVLAQIGCNIQEGNNYIEITGTSNPQGIDVDMRDIPDTAQTL
                     AAIAPFAVSPTRIRGIASARLKETDRIHATCTELARLGVSVEEHEDGMTIYPCEKFNH
                     ATIQTYNDHRMAMAFSLIGLRVEGVTIENPSCVSKTFPNFFAVLDRLQIPSPIGRGVG
                     "
     gene            complement(9658..10512)
                     /locus_tag="VFQ23_01580"
     CDS             complement(9658..10512)
                     /locus_tag="VFQ23_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET9905291.1"
                     /translation="MEDGFRLQDSRIAIIGLGLMGGSLALALKGKTAALYGIDAHPAT
                     LDLALAHQIVDEVDTKPDKLIPQADIIILATPVPAIIDLLEELPSFTQNSCIVLDLGS
                     TKKDIVAAMSTLPDWFDPIGGHPICGKEILGLENAEGKLYRDASFVITPLQRTTTRAK
                     SAAKQILSVIGSQFIEMTAEEHDRALAFTSHLPFLLSSSLARSTSAEFSSLIGPGFRS
                     TSRLAGTPSHIMMGILKSNRNNVLDAIQNFYHSLNEIELALQSENYSELEVIIDQSRA
                     AYHSLIEN"
     gene            complement(10517..11563)
                     /gene="aroF"
                     /locus_tag="VFQ23_01585"
     CDS             complement(10517..11563)
                     /gene="aroF"
                     /locus_tag="VFQ23_01585"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9905292.1"
                     /translation="MIIVMKPNYTPQQLEGVVLAVKAYGLTPHITYGVETAIVAAVGE
                     FHVPALDAFEILDGVEAVKRISQPFKLGSRQVHPENSVFPIDGFTVGGDDIVIIAGPC
                     AVESRSQILETAQAVREAGANALRGGVFKPRTSPYSFQGLGEEGLELLAEAREQTGMP
                     IVVEIMSQTQLELMVKYVDVLQVGARNMQNFNLLRAIGETRKSVLLKRGLSATIEELL
                     MSAEYILAGGNKQVMLCERGIRTFETSTRNTTDINAIPVLKNLTHLPVILDPSHATGH
                     ADYVAAIARAGIAAGADGLIVEVHPDPAHAASDGKQSLKPEAFAHMVKQVKAIAEVMG
                     RRIAPIKKPVVAGW"
     gene            complement(11710..12081)
                     /gene="aroH"
                     /locus_tag="VFQ23_01590"
     CDS             complement(11710..12081)
                     /gene="aroH"
                     /locus_tag="VFQ23_01590"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET9905293.1"
                     /translation="MPIRGIRGAITVSADEPELILQATRELLEEILAENEGMKPEEVA
                     SAIFTVTDDLASTFPAQGARQMGWGLVPMLCAREIPVPNSLPRVIRVLVHWNTELPQS
                     EITHVYLREAVKLRPDLVAAQ"
     gene            complement(12502..13269)
                     /locus_tag="VFQ23_01595"
     CDS             complement(12502..13269)
                     /locus_tag="VFQ23_01595"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905294.1"
                     /translation="MNVNEWGAALLTSITAALSLFLAAIPRIIGFAVILIIGWFIAGL
                     LAAGVAALLRAVKFNELAQSSGFSGFVRNMGVRKDASGMLADIVKWFVRLIVLVVAFD
                     ALGLPAVSQVLQQFLLWIPNLVVAVVVLVIAGLAANALGNLVRGSTASAGFSNPDLLA
                     SIARVAVWGFGIVVAVKQIGVAQTLVNTLFMGLIGAFALAAGLAFGLGGRETAGQIVD
                     GWYHRSREAAPKIERATENMARNMEGNRAIRDTEGTR"
     gene            13718..13891
                     /locus_tag="VFQ23_01600"
     CDS             13718..13891
                     /locus_tag="VFQ23_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905295.1"
                     /translation="MTEPGEVLLAENLALQSRTRAEGYQKCIADESIWRLIAAILVAA
                     QIKKPSIIFQIRL"
     gene            complement(13911..14651)
                     /locus_tag="VFQ23_01605"
     CDS             complement(13911..14651)
                     /locus_tag="VFQ23_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9905296.1"
                     /translation="MNTKRFSTSLLSLLVTSALLLSACGPRVTVTASQDPVTVVENFY
                     EIINMKDVEQAMTLTADDYVLTDTFGTYDKSVAAIQWQAAVDADRTFKQTDLVDTGNG
                     RVTSCYEIFENGKSIDRGCGKVSRVREGKIIFDGLLPAEGLWIVQQYYGLINAGDLEA
                     SMSFIADDVKCRGACYLTGKAAYQMYIQGIINGGGRKELRDFAVDGDKVTYYWAFYKK
                     DGRDVVISGIEFMQFKDGKIILIESDFN"
     gene            complement(14709..15011)
                     /locus_tag="VFQ23_01610"
     CDS             complement(14709..15011)
                     /locus_tag="VFQ23_01610"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9905297.1"
                     /translation="MILEVAILDVIPGKETEFEAAFVQAAPIIASMDGYVSHQLQKCL
                     ERQYRYILLVNWETLEAHTVGFRGSDQYQEWKRLLHHFYDPFPIVEHYELIQDHDE"
     gene            complement(15040..15636)
                     /locus_tag="VFQ23_01615"
     CDS             complement(15040..15636)
                     /locus_tag="VFQ23_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9905298.1"
                     /translation="MTKISPLVAIMNYYRAANAEVLKKVSELSDEQLAWRPHPACNSV
                     AFLLWHMARWEDHMQATVPGMTKELARRLPPSQQIWERDQISKKWGFAELQLGELDLG
                     TGFDRNVSEEPPWPGKEALLDYAQQTFATVEHAISFIDEEQFEEIERSEFDNEYMQGT
                     MEESVTVGNAVMEHLVHTYHHLGEIYYLVGLMKAGQTK"
     gene            16017..17207
                     /locus_tag="VFQ23_01620"
     CDS             16017..17207
                     /locus_tag="VFQ23_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905299.1"
                     /translation="MTSFTEKIDEWMKEAEERPESAVTIVRLIARRLRELTERDEELL
                     AENIALQNGTRVEEYQKRIAHLEYQLDLLKRRFALGDGALLEAVEESADISTINLLVY
                     NSYGRILRVELDANQPTLGRITDQRMNDREPPRILCIASNEEVLLLFTSGRVSTHKVS
                     DIPAVDVGGQWSWERAALPDEPRAGEWLACIMPFSHLPLTDYFLQVSRRGCVKNTMTS
                     LAQSVLSNHYLGKGTIQKSDQPLDVILGQKKDRFVFVTYEGKLLGLDVDELSYSAEER
                     IRLTTTDFVVASFLAQPDNTMLFFTQTGKVIQRESDSLELSKSPLAKGQPLISPSRLD
                     QGVRFVGAASVKDDDRVAVLDASGSLTVHVAESITGAGSIEAEGLILAVSVIPADAAP
                     RTES"
     gene            17204..18547
                     /locus_tag="VFQ23_01625"
     CDS             17204..18547
                     /locus_tag="VFQ23_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9905300.1"
                     /translation="MSLKVGIDFGTSNSGVAFYDGQRVQLLPVDPKNVLPEVIKTVLY
                     ITRDYRTYLGQEAVETYYRDNVNRQRRYVKQWAGEIDVFGADMHYVRDIYVYVDELKP
                     GRLLQYLKTALRKDNYQGTQIFERYYSVGDLAQTYLQLLKKRAEEVLGETIDSVTLGR
                     PVKFSESPERDHKAQETLRQAAHEAGFKEVDFELEPIAAALYYEQSITEPQNVVIFDF
                     GGGTLDIAVMRLGDEKSRQVFASGGVDIAGSDFDRTIIEKRMLPHFGYGKVKHHPEIM
                     DMIHAIPDWMALPELGTPINKNILDKAIQAKIAPVRLKALEGLIYNDLAFTFYNRVEA
                     GKIALSNDGATIISLEDKDISLWELYTRSQFESDIHHYLVDVEKVLLETIEKAGLDIE
                     EIDAVVKTGGSSNIPLFTEMLARIFGSEKVKESNAFSSVVAGLAIKAFDQSPLVE"
BASE COUNT         4849 a         4927 c         4583 g         4673 t
ORIGIN      
        1 cggtcgtatc tgcaatatat tgaagtatag gctcattaag ttgcgagtgg acggtaaagc
       61 cctctaccgt gatggtagct ccagccgcgc tgccaatccc gaccgtatag atacgcacgc
      121 ccaggtcagc tgccaggtcc gttgcggcaa tcgggtcagg tgactgattg ttttctccat
      181 cggtaagcag gaccaccaca gccgaggtat accagccttg cggagaagga gcggcggtcg
      241 gtgcctgccc gtcagacatg ctggtagtgg tcaggagtgg cggatcgccc gccgcgacag
      301 caatcgtatt gagcgccacc aaaatgccgt tgccaattga ggtaccacgt cgcgggacga
      361 gccgctcaat cgtggagatg gtttcctctc gattctctgt gggggcctgc actgaaatcc
      421 caccatcgct aaacgccaca accccaatca acacactggg cggctgcttg tcgacaaaat
      481 cgagagcggc taatttcgca gcttccatac gcgtgggttg caggtcatcc gcagacatac
      541 tgcccgaaac atcaaaggtc aggatgaccg tcccttcaat acgcggcaca ctgacggtcg
      601 cttgaggtcg agccattgag atcagtaaaa gcgtgattcc cgtcaaaaag atcatggcgg
      661 gcacgtgacg tcgaatgccg ggtgcttttc ctgtcgtgtt acgcgtgacg cccaggctgc
      721 caaattgcgc ggcaattttt tgtcgtcgct gctgaattcg taaataaaac agaatcaaga
      781 gcggtacaca gaacaacatc aaaagtaatg tgggccaggc aaatgacata gttataaaag
      841 ctccagaggg cgccgccggt accgcagagc gtcacctatt tgcgatactg cttccgttgc
      901 ttggcaaaac gcacaatggc gcgtaccaaa tcttcgtctg tggaaagcga caaaacatcc
      961 acaccggcgc gcttgaatgt actgttcaat gcctcttcgc gtttctgcgc cacttcaaaa
     1021 aaacgttttc ggaatttcgg atcatgcgta tccacaaaca gttgctcacc agtttctgca
     1081 tcctccatca cgaccatgcc gagatcaggc aactccactt cgcgcggatc ccagagtcga
     1141 atggcaagta cttcatgtct ttggttcagc aaactcaaag gcttttccca gccaggttca
     1201 ctaataaagt ctgagatgat gaaaatcaaa gagcgtcgct tgatcgtatt gagcgcaccc
     1261 tccagcatct ccttgagatt ggtaagcgga gcgcgggata agcgcggctg cctgagcaga
     1321 tcgttgataa gacgaaggac ctgcaatcga ccgcctcgcg cagggaccgt atgctgaacc
     1381 ttgtctccga acatgatcgc cccgatgcga ttcccatggc gggtcaacaa tcgtgcgatg
     1441 accgtgacaa aatcgacaag catcgtccgc ttttcgtttt gggactgcac cgcgccaaag
     1501 tccaccgatg ggctgaggtc cagcaggaac caggccgtga tctcgcgatc ttccacgtat
     1561 tgcctcacgt acggcgtatc catgcgcgcg gtcacgttcc agtcgatata gcggatgtca
     1621 tcctcaggct gatattcgcg caggtcggca aaatccacgc cgaagccata aaacagcgtg
     1681 cgataatcac cctgcaacaa gccatccagc ctgcggatga cattccaatc taaacgaaga
     1741 agaatttttt caggcgttac tgccgacgtt ggcatgctca tgtaaaggca ccaccgggat
     1801 tgggatacga tcaagaattc tttccagaat atggtcaccg gtaacatttt ctgaaagggc
     1861 ttcatacgat aacacgacgc gatgtctcat gacatctaaa gccatgtcca gaatatcctg
     1921 cggtaaggcg tagttacgac cgcgtacaaa ggccagcgca cgcccggtca ggatcaaatt
     1981 gatcgaagca cgcggactgg caccgaacat aataaagcgc tgcaaatcct tcaaaccgac
     2041 atcctgaggt ttacgcgtcg cagtcaccat ccgtaccgca tactccatca gcgccggatc
     2101 gacatacaca cgatctacat ctttttgcaa tgcaagcaat tgatctgtag tgagcacttt
     2161 ttgtaccgcc tgcagcatcc ctgtcatacg ttcaacgatg acaaattcct cggtcgaatt
     2221 tggatacccc accagcacct ttagcatgaa acggtccacc tgggcttcag gcagagcgta
     2281 ggtcccttcg gtctcaattg ggttttgtgt ggctaacaca agaaaaggat ctggcacttt
     2341 gaaagtttca cgtccaatgg tcacctgtcg ctcctgcatc acttcaagca gggcgctctg
     2401 cactttggcg ggtgcgcggt tgatttcatc agccagcaga aggttcgtga aaacgggacc
     2461 cagggaagtt tcaaacttac cagatttctg gttatagatg cgtgttccga ttaagtccgc
     2521 ggggacaaga tctggcgtga actgaatccg tttgaactcg ccgccgatcg actccgccag
     2581 agtcttgatc gccatcgtct ttgccaaccc tggaacacct tcaacgagaa cgtgaccgcg
     2641 tgctaaaagc gcaaccacca ttcgttccag taaatgatcc tgcccgacaa tgatcttttt
     2701 tacttcatat agaactttct ccattggtgt tcggttttca gggtttttgt tttgagccat
     2761 tgaatgtctc cttcaggatg atgttataca actcgatgta tcagcagtcg tactgctggt
     2821 acatcatata tttaataagg cggcgaaccg agcccacccg ctgcagtacc gatcggcacg
     2881 gcaaaaccga ttcccacaaa aaaactatct gcggttggat tgataattcc agtaacgatc
     2941 ccaatcacat gaccgttccg attcagcagc ggtccgcccg aattccctgg gttcgccgcg
     3001 gcatcgaatt ggatcaatcc ttcaatgggc tgtttgccat tgggtggctg gaacgtacga
     3061 ttgaagcctg agataattcc ttcgctcatc gaactatata acccaaaggg gttgcccacc
     3121 acgtaggctt catcacccac tagcatcgag ccaggattcc ctaacgtagc cgggaaccac
     3181 acctctggca tggtagacgt ccgcagggct gcgatatcac tctcaggctg ttcgttgaca
     3241 atggctgctt ctgcgcgcgt accgtctgca aaagtcacga cgattcgagt ggcatcgtct
     3301 accacatgta agctggttag aatgcttcct gtgtcatcaa cgatcacacc cgttcccagg
     3361 cccctgtctg tcacggtgcc ttcgcctcca aattccgatt cgatcatcac cagggaaggc
     3421 tgtatggcct gatatacctg gacggaataa gcagcacgcg gcgttgcaga agcaagggcg
     3481 ccggcaatgg cattactcac ttcagcttca gttacttgac tggcaggaaa gaggaaattg
     3541 tagagaaaaa aagccaggaa agccgcgaca actccggaaa ggaacggaaa cattcccttc
     3601 acgcgttcac gcagacttcc taatcggctt tgtggcttgt cagtggagac agatgattcg
     3661 ttcattgcct tccattcatt acaaactgta tttctactaa acagtcattc aacgaatagt
     3721 tgagagaact tcactaatca atcttacagg caattatgaa aatttgcaat tagaccctga
     3781 tgagaaaact ttggtgacct ggagcttcga gccacctctc aatttaggac tcgacgcaac
     3841 gagccgtaca acccttacta tacgactctc ctacgcttct caatcaagca ggcttggact
     3901 ctaccagtta ataatatacg tatcctgccc ctttacggat ttgccgctat ttctaccaat
     3961 gcccttaacc ccaatgtata agacctccag cctaacccaa gcaccttccc cttgcaaacc
     4021 gcagaaatca gtgacgtatg tctgaattct tcccgcgcgt acacattcga aatgtggacc
     4081 tcgatcactg gaatgccaat cgcggagatt gcatcccgca aggcaacgga tgtatgggta
     4141 tagccacccg ggttaaagat taccccattc gcccaggtgc gcgcatcgtg cagggcgtcg
     4201 atgagcgcac cttcgtgatt cgattgagag cactttactt ccgcacccag ttcgttacct
     4261 aattcaatta gcttattatt gatgtcatcc agagtcattg acccatacac ctctggctcg
     4321 cgcgtgccga gtaaattcaa atttggtcca tgtaaaatca atactttcat taatcctcac
     4381 tataacgtca atgtgaagag agctcttccc tggcaccctg tggcttatta atcagtacca
     4441 atgcaggtga gcattctcta tatagggaga ttgctccgct tcgctcgcaa cacttgcacc
     4501 tgacgcacac cgccccggtg ggtcggcgcc ggggagtgca ggtgtgacat caatccagca
     4561 ccttttccaa atcggttact tctaccaatt ccactttgcc aatctcaacc ggcaatgcaa
     4621 agcgaatgac cctggcatct ttctttttat ccactttcat ggcgcgtacg atttcatcgc
     4681 gcgataactc tttggagatt tgggtcggca atccaattgt agaaagcact tcaacaattt
     4741 tatctgtcaa ccctttactt gccacaccga ttcgttccgc atacctggct tcggtgacca
     4801 atcctatcgc aaccgcttct ccgtgacgta actggaattt gctagccaac tctacggcat
     4861 gaccaactgt atgccccaga ttcaatgcag cgcgaaatcc tttttcgtac ggatcctctt
     4921 cgataatctt tatcttcact gccatcgcac gcttgacgat ctgttcgaga ttctctttta
     4981 cgaaatccaa cccacgcgca cataaattga atagttcagg atcagaaatg atcccgtgtt
     5041 ttattacttc tgccagcccg gaaataaact ctctttcagg taatgtaatc aataactgtg
     5101 gatctgccag aacaagtttt ggcggataaa aagaaccgac cagatttttc ccttcgggta
     5161 aatcgatccc ggtttttcct cccagagagg catcgaccat ggaaagcaat gtggttggaa
     5221 tgccaaccca gctgatgcct cgcatgtacg tggaagcggc aaagcccgcc atatcaccaa
     5281 tgacaccgcc acctaatgca atgaccgtac tcctgcggtc aagaccctgc gccaaaaatg
     5341 atttccacaa gaaagaaacg gtattcaaat tcttaaattc ttcaccggct ggaaccgtca
     5401 aaatactcgg agcagagcct gacttactca aggaagccac aattttctct gcgtaaaatg
     5461 tcaggttttc atctgtgacg ataaggggat tcttgagatc tcgcgcggac agcaaactgg
     5521 caacttgctc caccgattcc acaatcacat catattcacc cattgctcga agatgaaacc
     5581 tgccaagcac gatttgtgcg cgtttggcat tctcttcagc agttccctga tcgacaacta
     5641 accgcatcgg aaacgagtgg taatgtcctt tgcgcacatc aagcaacgac gttaacttct
     5701 ccagtaaacc tcctgccagg agaggccgct gccgggaact ttcatttaat cgcttgaaca
     5761 gggtttccag ttccgccatg agtaatatta ctttgccatg ggtttcgacc agattcctat
     5821 tctcattacg tagtaatgct cccccaccta aagcgataac actttcactt tgtttgagta
     5881 ccttcttcag tgccgatgtc tcgagttcac gaaagctctc ctcgccctgc tgtcccatga
     5941 tctgcggaat ggatctgccg gcgttagact ccacgacacg atccagatca acaaatggca
     6001 agttgaggtt gcgcgacaga atccgcccaa ctgtagattt acctgttccc gggggaccgt
     6061 agaggaatat atgaggcatc gttattgaaa tcggtggtcg agtagacacg agcgtagcaa
     6121 gtgtcatatc gagactacca tacaagcaaa atgactttcg ttatcttttg gtttcgatac
     6181 gcctttcgtt atcactccag gctactcaac caacggttta ctcccaaaaa acatgtggaa
     6241 cattatccat ttgcaaatct tccagcctgg ctttgcgaag cgattcaaag cgtggtctca
     6301 tttcatccag cgagtcaccg cccagttttt cgatcaacgc atccgcaagg acgaacgcca
     6361 ccatcgcttc caggataggc acggcgcgcg gcacgggaca aaaatcggag cgttcatagt
     6421 tcgttggaga ttcctgcccc ttcgccagat ccacagtctg ctgaggcgtc aaggtcgtag
     6481 caattggttt cattgccgcg cgaatgacaa taggttgtcc attggagatt ccgccttccg
     6541 tgccgcccgc gcgattggtt aagcggtgaa tcgccctcac ccctgccccc tctccctcag
     6601 agagagggga aagaccgatt ggatcatgag cctgtgtacc gggcagcctc gcatttgcaa
     6661 acgcgtcacc gacttcgact cctttgatgg cttgtacgct catgatcgcc aacgcaagct
     6721 ttgcttcaag acgtttatcc cattgcataa aactaccgag accaaccgga agattcaaag
     6781 cgacgatttc gagcacacca cctagtgtat tctttccgtg aatcgtccgt tcgattgctt
     6841 cgcgcatttg atacgcagac gtgccaatag ggcatctcac atctgattgt tccgctcggg
     6901 cgaatcgctc ctcatagggc atctgaccaa aatcagcctg gatttcacca atggatgaaa
     6961 catagccacc cacgataatt ccaaactgcg ataagaagtg tttacagacc gcgccgaccg
     7021 cgactcgcat ggtcgtctct cttgcggaag cacgttccag cgctggacgc aaatccttat
     7081 acccatactt taccgcaccg gtcagatcag cgtgtccggg acgcggggcg gtcatcggct
     7141 cgatggcttt gcccttccat ttgacatgat ccgcattttg tacgagcagt gcgatcggcg
     7201 cgcctgtcgt ttcaccagcc ataaccccgc caagaatttg cacagagtct ttttcgatct
     7261 tcatacgtcc gcctgaaccg tagccttttt gacggcgcgc aagctcaggg tcaatgatcg
     7321 aggggtccag tgtaatgcca gcaggcatac cgtcgaggat ggctgttagg gaaggtccgt
     7381 gggattcacc ggcagtgaga aagcgtaaca taggcattaa ttagagacag gggagccttc
     7441 aacggatgcg cgcatgattt ctcgcgaggg gttatgccca gtccacagtt caaacgctaa
     7501 agcggcctgt tcgatcagca tcccaagtcc ggttgtggca ttgagtcctc gtgcccgcgc
     7561 ctccttcact aattttgtct ctcttggatt gtagaccaaa tcgtacatcg aaacatgacg
     7621 aggcagtgac aggttctcag gcagaggaga ttggtctatg tttggcgtca taccgactgg
     7681 ggttgtattg acgatcagtg cgattttcga gaactccaca tctgtgaaac tggaaaccac
     7741 atctggtgat ttggtattag caattgaatc agcaagatgc tgagcttgtt cgattctacg
     7801 cgcggcaatg attacatccc agccgtcatt taataatgca tacaccacag ctcgtgctga
     7861 ccctcccgca cccaggataa gagcgctttt cgattcgttg ctctgctccg cggtggctag
     7921 gaaattcttc aggtcagtta gaaaccctgc cgcatcggta ttatcgccga taagcttgcc
     7981 ttcacgcaaa taaattgtat tgaccgcacc gatagcttca gaagtcgatg tcaacacatc
     8041 aaggaatgaa attacatttt gtttatgcgg aatggtgacg ttaagcccat ggatttcccc
     8101 ggaacggacc cgagacaaca agtcctgcaa tccgttttta tcctcagggt agatcgggaa
     8161 gagagaatag tccccttcga atccacacgc tctcagcgcg gcggaatgaa tgtttggcga
     8221 gagggaatga ctgagaggat atccaatgag tcccaaatta aaaagaggca tgaatcgccc
     8281 tcaccctacc cctctcccaa tgggagaggg gatttgcagc ctgtcaagca ctgcaaagaa
     8341 attaggaaat gtttttgaga cgcaggacgg attctcaatc gtcacccctt ctactcgtaa
     8401 accgatcaat gaaaatgcca ttgccatccg gtggtcgtta taggtttgga tagtggcatg
     8461 gttaaatttc tcacacggat aaatcgtcat accatcctcg tgttcttcca cagagacgcc
     8521 aagccgcgca agttcagtgc aggtcgcgtg gatacggtcg gtctctttca gacgcgccga
     8581 cgcgatgccg cgaatccttg tcggcgagac tgcaaagggg gcaattgccg cgagcgtttg
     8641 agcggtatca gggatatcgc gcatatcgac atcgataccc tggggatttg acgtgcctgt
     8701 gatttcgatg tagttatttc cttcctggat attgcaaccg atttgcgcta aaacatcgac
     8761 aaagttaata tccccttgtt tggatttgcg tgaaatattc tccactcgga ttttgccacc
     8821 acagatggcg ggtgccgcaa agaagtatga agctgcggag gcgtcggatt cgatgttata
     8881 ggtagagagt ggcgaataag aattgggatg tatagtgaat tgcgaatact cccggcgttc
     8941 aacttcgaca ccaaagtctc tcatcatggc aatcgtcaga tcgacataag gcttggagtt
     9001 gagatcggtc gtgacttcga tttctatcgg agaatttgcg tagggagcga tcatcagtaa
     9061 cgccgacaaa aactgcgatg aaatatttcc ggctattttt gttttaccgc cgggtaatcc
     9121 ggatgcaatg attttgatcg gagggcaaat ggaagctgct actctttccc catccaccct
     9181 ctccatcaaa gagagagggc tcaattgcgc gccgagttga gtgagagcgg ttacgagatc
     9241 tccaatggga cgctctctca tccttgcatc gccatctaaa acatactcac cgtttcccag
     9301 ggtaagaaag gccgatagaa aacgcgcggc tgtccccgca ttgccaatga agagttcggc
     9361 tttgttcgca gggattattc cgccctggcc tgtgatggtc attttgtgat tatgctcgtc
     9421 aagttcgatg tcgaagccca gcgtttgcaa cgctctggca aaataagacg aatcatcgga
     9481 gaagagtgcg ttcgtaaggc gcgtttttcc attcgcaagc gaactgataa gcagcgcacg
     9541 gttcgtcagt gactttgagc ccggcacgcg tactgttgct tgaagtggat attcaatcga
     9601 ttttatctgt atggatggca aagtcgcgag catagataat gaaatttatt tactcaatca
     9661 gttctcgatc agcgaatggt aagctgcccg tgactgatct attatgactt caagttcact
     9721 gtaattttca ctttgcaggg caagttcaat ttcattaagt gaatgataga agttttgaat
     9781 agcgtcgagg acattgtttc gattggattt caggataccc atcataatat gagaaggtgt
     9841 acccgcgagt cgactcgtgg aacggaaacc cggaccaatc aacgaggaaa actctgcaga
     9901 agtggaacga gccagggagg atgaaagaag aaaagggaga tgactggtga aggcgagagc
     9961 gcgatcatgt tcttcagccg tcatttcgat aaactgtgag ccaatcaccg agagaatctg
    10021 ttttgccgca cttttcgctc gcgtggtcgt tctttgcagt ggagttatca caaaggaagc
    10081 gtctcgatac agcttgcctt ccgcgttttc aagtcctagt atttctttgc cacagatggg
    10141 atgaccgcca atggggtcaa accagtcggg tagcgtagac atcgctgcca caatatcttt
    10201 tttcgttgag ccaaggtcca ggacaatgca tgaattttgt gtaaaggatg gcaattcttc
    10261 gagtaagtca ataatcgcgg gaacgggtgt tgccaggata atgatatccg cttgagggat
    10321 aagtttatct ggtttggtat cgacctcatc cacaatttga tgggcaagag caagatcaag
    10381 cgtcgcaggg tgtgcgtcaa tcccatacag tgcggcggtt tttcctttta atgccagggc
    10441 gagcgaacca cccatcaggc cgagtccaat gattgcgatc ctggagtctt gtaatcgaaa
    10501 gccatcctcc attttattac cacccggcaa cgacaggttt cttgatggga gcaatacggc
    10561 ggcccatcac ctcggcgatg gctttgacct gtttgaccat gtgggcaaaa gcttccggtt
    10621 taagtgactg cttgccatcc gaggcggcat gtgccgggtc ggggtgtact tcaacgatca
    10681 gaccatcggc accagcagcg atcccagccc gggcaatcgc cgcgacataa tcggcatgac
    10741 cagtcgcatg agatgggtca aggatcacgg gcagatgcgt aagatttttc agaacaggaa
    10801 ttgcattgat atccgtcgta ttccttgtgg acgtttcaaa ggtgcggatg ccgcgctcac
    10861 acagcatcac ctgtttgttg ccccctgcca aaatatactc agcagacatg agcaattctt
    10921 caattgtggc acttaagccg cgtttgagca ggacactctt gcgcgtctca ccgatggcgc
    10981 gcagcagatt gaagttttgc atgttgcgtg cgcccacctg caacacatct acatacttca
    11041 ccattagctc caattgagtc tgagacatga tctcgaccac aatgggcatg ccagtttgtt
    11101 cgcgcgcttc tgcgagtaat tccaatccct cttcacccag accctggaaa gagtacggtg
    11161 aggtacgcgg tttgaaaact cctccacgca aggcattggc tccagcctca cggacagcct
    11221 gagcggtctc gagaatctgc gaacgagatt ccacggcaca aggtccggcg atgatgacaa
    11281 tgtcgtcgcc tcccacagtg aagccatcaa tgggaaacac tgagttttcc gggtgaacct
    11341 gacgtgaacc aagtttgaac ggttgtgaga tgcgtttcac ggcttcgact ccatccagaa
    11401 tttcaaaggc atccaaagcg gggacgtgaa attcgcccac cgcggcgacg atcgctgttt
    11461 ccactccata ggtgatatga ggggttagtc cgtaggcctt gactgccagg acaacacctt
    11521 caagttgctg cggcgtgtaa ttgggtttca ttacgatgat catgatttct tctcctattt
    11581 gttctgctgg ttgagacgta agcagtcgaa accagcatat ttacaagaaa gttttcgttt
    11641 gctagttact ggtggtttcg atacggaacg tccgaacacc gttcctactc aaccaccaaa
    11701 attttcttac tattgcgcgg caaccaagtc gggtcttagt tttaccgctt cacgcaaata
    11761 aacgtgcgtg atttcacttt ggggcagttc tgtgttccaa tgaaccagaa cacgaatcac
    11821 tctcggcaat gagtttggaa cagggatctc gcgcgcacaa agcatgggga cgagtcccca
    11881 gcccatttga cgggcaccct gtgcggggaa ggtggaggcg aggtcatctg tcacagtaaa
    11941 aattgcactg gcaacctctt caggtttcat tccctcgttc tctgcaagaa tttcttcaag
    12001 caactcacgc gtggcttgaa ggatcaattc cggttcatct gctgagactg tgatggcacc
    12061 gcgtattcca cgaattggca tagattgctc ctatgaagtc tcatccctgg cccctctccc
    12121 tccttagagg aggtggggag gaagaggggg tgaggagaga aaatgaaaga gcgagaccgt
    12181 gtggtctcgc tctctttgtg aacagatgta tttcctgtct acttaagcgc caccaacggc
    12241 aaccgggtcc cggaaaccgt aataaaagta aaacgcaaac caacgagtgg acttaagcat
    12301 attaggcgct attctatgcc agtgacgaaa atgcgtcaag aggcgatttt ataaggtcta
    12361 taactctatt aacgttgaaa ccttcataat tccagcactg gactatgaat tccattcacc
    12421 cggtacggtg atacttaaaa cagatctggc tgaccatcaa catggtcagc cagatgtagt
    12481 ctttcctttc acaggaaggt actagcgcgt tccttctgtg tcgcgtatgg ctctgttgcc
    12541 ttccatattg cgcgccatgt tttctgtagc acgttctatc ttgggggccg cttcgcggct
    12601 gcgatgatac cagccgtcca cgatttgccc ggcggtctca cgtccaccca gcccaaaggc
    12661 taagccagcg gctaatgcga aagcgccaat caaacccatg aagagcgtgt tgactaatgt
    12721 ctgcgcaaca ccaatctgtt tgaccgcgac cacaattccg aatccccaca ctgccacacg
    12781 tgcaatcgaa gccagtaagt ccgggttgct aaaccctgcc gatgcagtcg agccgcgcac
    12841 cagattaccc agggcattgg cagccaaacc agcaattacg aggactacca cagcgaccac
    12901 caggtttggg atccacagca ggaattgctg aaggacctgg gaaacggccg gtagacccaa
    12961 tgcatcaaag gccacgacta gaacgatcag acgcacaaac catttgacga tatctgccag
    13021 catcccggaa gcatccttac gcacacccat attgcgtacg aatccactga acccggaact
    13081 ctgagccaat tcgttaaact ttacggcgcg cagtagggct gccactccag cggcaagcaa
    13141 acccgcgata aaccaaccga taataaggat cactgcaaag ccgatgatcc gcggaatagc
    13201 tgctagaaac aaactcagtg ccgcagtaat tgatgttagg agtgcggctc cccattcatt
    13261 aacgttcatt gatatgttcc tttcatcctt gccatttgag ggcaggtgaa tatatagctc
    13321 gaggagatcg aacttcgttt ttttcaattt accaaaatgt ttctaatcca ctcaagacct
    13381 gatccgtttt ttcacttatg tggacaatac tcctcagtgt cctgtttctc aattcgactg
    13441 ggatcaataa taagtgacta tctggatgga ttatgataat aaactgagat atggtcaata
    13501 agggagttac ccccgggcta gtgattaccc tacttgccaa atcgtcaagt tccatgaacc
    13561 aaatcccggc gaaagacgac cttaaattct aattattggt tcaaggaatc tcaactctgt
    13621 ccttcaacag caaaatcgaa gaatggatga aagcagccga aaagcgactg agtccgcggc
    13681 tacgactgtg aggctcatcg tcaggcaatg acgtccgatg accgagccgg gtgaagtact
    13741 gctggcggaa aaccttgctc tgcaaagcag aacgcgcgcc gaagggtatc aaaagtgtat
    13801 tgcggatgaa tctatttggc gtttgatcgc cgcgatttta gttgccgcac aaatcaaaaa
    13861 gccctcgatc attttccaaa tcaggctgtg aggcgtgtaa tgtcacaggg ttaattgaag
    13921 tcgctttcga tcagaataat tttgccatct ttgaactgca tgaactcgat gccactgata
    13981 acgacatcac gaccatcttt tttgtaaaac gcccagtaat aggtcacttt gtcgccatcc
    14041 actgcaaaat ctctgagctc ctttcgtcca ccgccattga taataccttg aatatacatt
    14101 tgatacgccg ctttgccggt gaggtaacac gcgccgcggc atttcacatc atcggctatg
    14161 aacgacatgg aggcttccag atcaccggca tttatcagcc catagtattg ttgaacgatc
    14221 caaagcccct cagcgggtaa taacccatca aaaataatct tgccctcgcg aacgcgactc
    14281 actttgccac agcctctatc aatggacttg ccgttctcga agatttcgta gcacgaggtc
    14341 acgcgaccat tccccgtatc gacaaggtct gtctgtttga aggtacggtc agcatctact
    14401 gctgcctgcc attggatcgc ggcgacggat ttatcatacg taccaaaggt atcggtcagc
    14461 acataatcat cagccgtcaa tgtcatggcc tgttcaacat ccttcatgtt gataatttca
    14521 taaaagtttt cgacgacggt caccgggtct tggctggcgg tgactgtgac cctcgggcca
    14581 cacgcgctta gcaagagagc tgaagtcacc aaaaggctca atagtgaagt tgaaaagcgt
    14641 ttggtgttca tgctgtaatc tccttcaatg aaaatacaac aagccagaca aatcgaattt
    14701 accgtgaact attcgtcatg atcctgaatc aactcataat gctcaacgat tggaaacggg
    14761 tcgtaaaagt gatgcaacag cctcttccac tcctgatact gatccgaacc acggaagccg
    14821 acggtatgtg cttccaacgt ctcccaattg accagtagaa tgtaccgata ttgtctctcg
    14881 aggcatttct gaagctgatg agagacatag ccatccatgc tggcgatgat cggtgcagcc
    14941 tgcacaaacg cggcttcaaa ttcggtttct ttgccgggga tgacatcgag gattgcgact
    15001 tccaggatca tttgttgata ttctccacaa gctaaacttc tatttcgtct gtccggcttt
    15061 catcaacccc accaggtaat aaatttctcc caaatgatga taggtgtgaa cgagatgctc
    15121 cataaccgca ttgcccaccg tgactgattc ttccatggtc ccctgcatgt actcattgtc
    15181 aaattccgag cgttcgatct cctcgaattg ttcctcatcg ataaaactga tggcgtgctc
    15241 cacagtcgca aacgtctgtt gggcatagtc gagcagagct tccttcccgg gccagggcgg
    15301 ctcctcggag acgttgcggt caaacccggt gcccagatct agttctccaa gttgcaattc
    15361 agcgaaaccc cacttctttg aaatttgatc cctttcccaa atttgctgac tcggcggcag
    15421 gcgtcgagcg agttctttgg tcatgcctgg aactgtggct tgcatatgat cttcccagcg
    15481 agccatgtgc caaagaagaa aagcgaccga attacaggcg gggtgaggtc gccaggcaag
    15541 ctgttcatcc gatagctcac tgactttttt caggacctcg gcatttgcgg cccgatagta
    15601 attcatgatg gcgaccagtg gactgatctt agtcatgttg aaccctccaa aactgaactg
    15661 gtgcgactga attcaataga gtctaatctt acatcttttc ctgcaaattc ggcagtttca
    15721 actcaaaaga gagtgtctaa tttagtgggc ttagccacac acctgcctga tttgctcgag
    15781 caaatcacgg gcggtgccag gggagcccac agagtctttt gagttttttc cacctgcccc
    15841 gaagcaggtg cggggtcaag gaactcagtg atccacctgc actggcatcc aggtgcgagt
    15901 gtctgtggca aaacagctac tagcccatac ttttggacac tcccacccaa aacagaaaat
    15961 ttctggatac aaaaattctc ctcatgtata attacgagtt cgaggaatcc caaaccatga
    16021 cttcattcac cgaaaaaatt gacgaatgga tgaaagaagc cgaagagcga cctgagtccg
    16081 cggttacgat tgtgaggctc atcgccaggc gtttgcgtga gttgacagag cgagatgagg
    16141 aactgctggc agaaaacatt gctctgcaaa atggaacgcg cgtggaggaa tatcaaaagc
    16201 gcatcgcaca tttggagtat caacttgatc tgctcaagcg tcgttttgct ttaggggatg
    16261 gtgcccttct ggaagcagtg gaggaatctg ctgatatctc aacgatcaat ctgctggtct
    16321 acaattctta tgggcgaatc ttgcgcgttg aactcgacgc caatcaaccc acgctgggtc
    16381 gtatcacaga tcaaaggatg aatgaccgcg aaccgccccg catcctttgt attgcatcca
    16441 acgaggaagt acttctcctt tttacatccg gaagagtaag tacgcataaa gtgagcgaca
    16501 ttcctgccgt ggatgtaggc ggccaatggt cttgggaaag agccgccttg ccagatgagc
    16561 cacgcgcagg cgaatggctg gcttgcatca tgccattctc acacctgcca ctcacggatt
    16621 actttcttca agtcagtcgt cgcggatgcg tcaagaatac gatgacatcc ctggcgcaat
    16681 ctgtcctctc caatcattac ctgggcaaag gtacgataca aaagtccgat caacccctgg
    16741 atgtcatcct cggtcagaag aaggatcgtt tcgtctttgt tacctacgaa ggtaaattac
    16801 ttgggctgga tgtggatgaa ttatcgtaca gcgcggaaga gcgcatccgc ctcactacaa
    16861 ctgattttgt cgtcgcatcc ttcctggctc aacccgataa caccatgctt ttcttcactc
    16921 aaaccggaaa agttattcag cgtgaaagcg atagccttga actatcgaaa tctcctctgg
    16981 caaaaggaca acccttgatc tcgccttcgc ggcttgacca gggagtgcga tttgtgggtg
    17041 cggcatctgt gaaagatgac gaccgcgtcg cagtgttgga tgcatcgggg agtctaaccg
    17101 tccatgtagc agaatccatc accggcgcag gttcgatcga agccgaaggc ttgatcctgg
    17161 ccgttagcgt gatcccagcg gacgccgcgc cgaggaccga gtcatgagtc tcaaagtcgg
    17221 cattgacttc ggtacatcca attcaggtgt cgcgttctac gatgggcaac gcgtccaatt
    17281 gctgccggtt gacccaaaaa acgtcctgcc ggaagtgatc aaaaccgttc tgtatatcac
    17341 gcgcgactat cgaacatatt taggacagga agcagtcgag acctattacc gcgataacgt
    17401 aaatcgtcag cgacgctatg tgaagcagtg ggcgggtgag attgacgtct ttggcgcgga
    17461 tatgcactat gtgcgcgata tttatgttta cgtagatgag ttaaaaccag ggcgtttgtt
    17521 gcaatatctg aagaccgcgc tacgtaagga taactatcag gggacacaaa tttttgaacg
    17581 ctattacagc gttggtgatt tagctcaaac atatttacag ttattgaaga aacgggcaga
    17641 agaggtgctg ggcgagacga ttgactctgt aactctggga cgccccgtca agttttcgga
    17701 gtcgcctgag cgggatcaca aagcacagga gactctgcgc caggcggcgc acgaagcagg
    17761 tttcaaagag gtggactttg agcttgagcc tattgcggcg gcgctatatt acgaacaatc
    17821 catcacagag ccacaaaacg tcgtcatctt tgactttggc ggcggcacgc tcgatatcgc
    17881 ggtgatgcgc ctcggcgatg agaaatcacg ccaggtattt gccagcggcg gcgtggacat
    17941 cgcaggcagt gacttcgatc gtacgattat tgagaagcgc atgttgccac atttcggata
    18001 cggaaaagtc aaacatcacc cggagatcat ggacatgatc cacgctattc cggactggat
    18061 ggcgctccct gaactgggca cacccatcaa caaaaatatt ctggacaaag cgattcaggc
    18121 aaagatcgct cctgtccgtt tgaaagcatt agaggggttg atctacaacg atctggcgtt
    18181 cacgttctat aaccgcgtcg aagccgggaa gatcgctctg tcgaatgatg gcgcaacgat
    18241 catcagcctg gaagataaag acatctcgct ctgggaactg tacacacgct ctcaatttga
    18301 atcggatatt catcattatc tggtggatgt ggaaaaagtt ttgttggaaa cgattgaaaa
    18361 ggcaggcctg gatatcgaag aaatcgatgc agtcgtaaag acgggaggat cgtcgaatat
    18421 cccattgttc acagaaatgc tagcgcgcat ttttggaagc gagaaagtaa aagaatccaa
    18481 cgcgttcagc agtgtggtgg cgggtctagc gattaaggcc ttcgatcagt ccccgttggt
    18541 cgagtagccc tatgcaagtc gggcatatcg agaccaacag attattacaa gaaattctgc
    18601 aacaaaattg ttttggttag tagtggtctt gatacgggcg agacgaacac tcgccattat
    18661 ggctttctca agaaagtttg acaaaatccc tttttgtccg cggatcacgc ggaggtcacg
    18721 gatttcccgt gaaaaaacgg aaaagcctcc gtcccttccg tgaaatccgt gtacaaaacc
    18781 acctaatctt gttcttgaga aaaccataca ctcgccctac tcgaccaccg gggcaatttg
    18841 gcgattatga taaaatccgc ccaatgctaa ggagtttgat atgaaacctg gcgattatgt
    18901 tggtttgtat attgacgata agttgatcta cacaggtgtt ttgaaagata ttggaaagat
    18961 ggagagcggg cgcacaattt accagatatt gctggtcaag aatatcttcc agcgcaacta
    19021 cccagaaatt ca
//
LOCUS       DASTJP010000053         3904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1471004, whole genome shotgun sequence.
ACCESSION   DASTJP010000053 DASTJP010000000
VERSION     DASTJP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471004"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..845
                     /locus_tag="VFQ23_01630"
     CDS             135..845
                     /locus_tag="VFQ23_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905301.1"
                     /translation="MNEKMQITVDPDGKWIYRVGGLSAIVLGIGYLLTFPVVIVYAGG
                     FPLPGAEAKLVHFAEHASDWWAATALMVFTDLLYVPVFLALYQALKGINKYMMLLALA
                     CEGLFVWLDLAITWTAYSSLTALGGVYAAVTSDAQRASIVAAAGYPSAITDSPLLGIY
                     IVFFPAVGFLLASLVMRKGVFNKAVAYMGMVSGISGILAGIGPIFLGALDTVQYINAG
                     LAMIWFFFVGFKLNKLGQ"
     gene            899..1873
                     /locus_tag="VFQ23_01635"
     CDS             899..1873
                     /locus_tag="VFQ23_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9905302.1"
                     /translation="MKAIVYTQYGSPDVLQLKEVVKPEPRNDQVLVKVHAASANALDH
                     RRFEKLSSMGRFMEERLGKSIGKALGADIAGVVEAIGANVKQFQPGDEVFGVAAGSKG
                     GFAEYASANEDHLTLKPANLSFEAAAAVPVAALAALQGLRDKGQLQPGQKVLIVGASG
                     GVGTFAVQIAKSFGAEVTAVCSARNLDMARSIGADNVIDYAKEDFTKSNQGYDLILAV
                     NGNYPLSAYRRALSPQGSYVCAGGAMSQILQAMLLGSLMSRKSGKKMGFMGIAKYNQK
                     DLIYIKELLESGKVAPVIEKSLPLCEVPVGMRYLAEGHAKGKVVFQIS"
     gene            1896..2393
                     /locus_tag="VFQ23_01640"
     CDS             1896..2393
                     /locus_tag="VFQ23_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905303.1"
                     /translation="MPAQNNLFMLFGPKGSGKTHIGTIVSQYTDILFLRVEPIWLGLK
                     PDEDGWEKVEAVIDAMFQKHDKVMIESLGVGEGFTKFHASLAEKYSIKLIRVYADLET
                     CFTRVKTRNKAEHIAVSDDKVTEFNKIAATVTYDWDLEINNNEFAVDEDIIRAIQSIN
                     GAENK"
     gene            2458..2958
                     /locus_tag="VFQ23_01645"
     CDS             2458..2958
                     /locus_tag="VFQ23_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905304.1"
                     /translation="MFQVKKLFLLIVLFAMGCASKPETPIKAVYLVREAGQLSREDLQ
                     THPEVVVTDNFDTLKKIAQTKVAIWIDINAVDLVDLEWLSQSPQALYPVVLVGNGDET
                     CSFFNTIHYFTFEMPCCLDCSSYPPGYSVNIQTDMSQGHMQGYEKTPTVQDILDITNP
                     LLEQVK"
     gene            3002..3325
                     /locus_tag="VFQ23_01650"
     CDS             3002..3325
                     /locus_tag="VFQ23_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905305.1"
                     /translation="MISRIWHGRTTLQNADKYEALLKDEIFVGIQSRLIRGFKGIQLL
                     RREVIGEVEFITIMTFESLDAVREFAGEDYERAVVPEKARAMLSHFDEQSQHYEIRME
                     MAGNG"
     gene            3520..>3904
                     /locus_tag="VFQ23_01655"
     CDS             3520..>3904
                     /locus_tag="VFQ23_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905306.1"
                     /translation="MFRGKSTFPLVVLFAVFLSSCAPGAEFNPLPGMTRMPGTDIPQQ
                     VDISIPDILTQTAVPVTSTPVPDGASPTPSSTGLKPPDSDTPAAGICGDAQSDPVSIV
                     LGMGPDGIPLAPRCIIITPGQRIKLI"
BASE COUNT         1022 a          892 c         1003 g          987 t
ORIGIN      
        1 ttcctgactg gaccggttgt tgaccaggcc gcgttgtttg gattgttgaa aaaaatacgc
       61 gatctgggac tgccgttggt ctcaatcgct cgcgtcgact ccatcaagtc agatattgaa
      121 aaaggagaaa tgaaatgaac gaaaaaatgc aaatcacagt tgacccggat gggaaatgga
      181 tctatcgcgt aggaggcctc tcggcgattg ttctgggaat agggtatctc ctgactttcc
      241 ccgtggtgat cgtttatgca ggtggctttc cgctgccagg tgctgaggca aagctggtcc
      301 atttcgccga acatgcatct gattggtggg ccgccacggc tctgatggtc tttacagacc
      361 tgctctatgt gcctgtcttc ctggcgctct atcaggccct gaagggaatc aacaaataca
      421 tgatgttgtt ggcgctcgcc tgcgaaggat tgttcgtctg gctggatctg gcaatcacct
      481 ggacagccta ttcttcgttg accgcgctgg gcggtgtcta tgccgcagtc acaagcgatg
      541 cccagcgagc gagcatcgtt gcggctgcag gttatccatc tgcgataact gattctccct
      601 tgttgggcat ctatattgta ttttttccag cagtgggatt tctcttagcc agcctggtca
      661 tgcgaaaagg ggttttcaac aaggcggtag cttatatggg aatggtctct ggcatcagcg
      721 gaatcctggc gggaataggt ccgatctttc tcggcgctct ggatacggtg caatacatca
      781 atgccgggct tgccatgatc tggttctttt tcgtaggttt caaactcaac aagctcggtc
      841 aataggcgaa taatcttaac catcctgccg cttgtgataa gtaatcacag gagaaaaaat
      901 gaaagcgatt gtttacaccc aatatggttc acctgatgtc cttcaactca aagaggttgt
      961 aaaacctgag cctaggaacg atcaagtcct tgtaaaagtt catgcagcgt cggcaaacgc
     1021 gctggatcat cgacgcttcg agaagttatc gtcaatggga cgtttcatgg aagaacggct
     1081 tggcaaatca atcggcaaag cgctcggcgc ggatattgcc ggagtcgttg aagccattgg
     1141 cgcgaacgta aaacaattcc agccgggaga tgaggtcttc ggagtagccg cagggtccaa
     1201 gggtgggttc gcggaatatg cttcagcaaa tgaagatcat ttaacgctga aaccagccaa
     1261 tctgtcattt gaggcagcgg ctgctgtccc tgttgcggca ttggccgcgt tgcagggact
     1321 ccgcgataag ggacagcttc aacccgggca aaaggttctc atcgttggag catctggcgg
     1381 cgtgggcaca tttgcagtgc agattgccaa atccttcggc gcggaagtga ccgccgtatg
     1441 cagtgcgcgg aatttggata tggcgcgctc gattggcgca gacaacgtta ttgattacgc
     1501 caaggaagat tttactaaaa gcaatcaggg ttatgacctg atcttagccg taaatggaaa
     1561 ttatcctctg tccgcgtatc ggcgcgcgct gagtccccag ggaagttatg tctgcgccgg
     1621 gggcgcaatg tctcaaattc ttcaagccat gcttttggga tctttgatgt cgaggaaaag
     1681 tgggaagaag atgggcttca tggggattgc gaaatacaat cagaaggatt tgatctacat
     1741 aaaggagctt cttgaatccg gcaaagtcgc gccggtcatc gaaaagagcc ttccattatg
     1801 cgaggttcct gtggggatgc gttatcttgc cgaaggtcac gcaaagggaa aagttgtttt
     1861 tcaaattagc tgacaaaaga aatcgagagt aagccatgcc tgctcaaaac aacctcttca
     1921 tgcttttcgg accgaaaggt tctggcaaga cccatatcgg gacgattgtc agtcaataca
     1981 ctgatatttt gtttctacgc gtcgagccga tctggttggg actaaaacct gacgaagacg
     2041 gatgggaaaa agtcgaagcg gtcattgacg cgatgtttca gaagcatgac aaggtcatga
     2101 tcgagagtct gggagttggg gaagggttta ccaaattcca tgcatcactt gcggaaaaat
     2161 attccatcaa gttgattcgc gtgtatgccg atttagagac ctgtttcacg cgcgtaaaaa
     2221 ccaggaacaa ggctgagcat attgccgttt cggatgataa agtcaccgag ttcaataaaa
     2281 tcgccgccac tgtaacctac gactgggatc ttgaaattaa caacaacgag ttcgctgtgg
     2341 acgaagacat tattcgggct attcaatcca tcaatggcgc cgaaaataaa taatattgaa
     2401 gcttaaacag agactgtttg tccagtcact cctatgtaaa cccaaaagga atagcaaatg
     2461 tttcaagtta aaaagctttt cttacttatc gtgttattcg ccatgggatg tgcctccaag
     2521 ccagaaacac ctattaaagc tgtttacctg gttcgagaag ctgggcagct ttcgcgcgag
     2581 gatctgcaaa cacaccccga agtggtggtc acagacaact tcgatacgtt gaaaaagatt
     2641 gcgcaaacga aagttgctat ttggatcgac attaacgcag ttgaccttgt ggatctggaa
     2701 tggctcagcc aaagtccaca agcgctttac ccggtagttc ttgttggaaa tggtgacgag
     2761 acatgttcct ttttcaatac aatacattac ttcacctttg agatgccatg ttgcctggac
     2821 tgtagttctt atcctcctgg ttatagtgtc aatatccaaa cagatatgtc gcagggacac
     2881 atgcagggat atgaaaagac accaactgta caggatattc tcgacattac aaacccgctc
     2941 ctggagcaag tcaagtaaaa catatcttat aacagcatgt tcttcacacg gaggagaaaa
     3001 aatgatcagc agaatttggc acggtcggac gactctgcaa aacgcggaca aatatgaagc
     3061 tctgctcaaa gacgaaatct ttgtcggtat tcaaagccga ctcatccgcg gcttcaaagg
     3121 cattcaatta ctgcgccgcg aagtcattgg cgaggtggag ttcatcacta tcatgacatt
     3181 cgaatcgctg gatgccgttc gtgagtttgc gggagaggat tatgaacggg cagtggttcc
     3241 ggaaaaagcg agagcaatgc tctcccactt tgatgaacag tcacaacatt atgaaatcag
     3301 aatggaaatg gcgggcaacg gataatactg taacagggat caatttgagc gatggccgtc
     3361 ggggttccga gtatgcggag tgtcattcgt ccatttgtgt aatatggaga ccatttgaag
     3421 ggtttggaac aaaaacgcgt gtaaatatgt cattatgaat attgctgacc gtagtaatca
     3481 tcttgtcaat aagccgagtc tccagaaagg gagaagcaaa tgtttcgagg aaaatcaacc
     3541 ttcccgcttg ttgtgctgtt tgccgttttt ctatcctctt gcgctccggg tgccgaattt
     3601 aatccgctgc ccggtatgac gcgcatgccg ggaaccgata ttccccaaca ggtggatata
     3661 tctatccccg acatcctcac tcaaacggcg gttccagtaa catccacgcc tgttcctgat
     3721 ggcgcttctc cgacgccttc ctcgactgga ttaaaaccac ctgacagcga cacacccgcc
     3781 gcgggtatct gtggcgacgc acagagtgat cctgtttcca ttgttttagg tatgggtccc
     3841 gatggaattc cactcgcacc acgatgcata ataatcacgc ctgggcagcg catcaagctt
     3901 atca
//
LOCUS       DASTJP010000054         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_781267, whole genome shotgun sequence.
ACCESSION   DASTJP010000054 DASTJP010000000
VERSION     DASTJP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781267"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFQ23_01660"
     CDS             complement(<1..529)
                     /locus_tag="VFQ23_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HET9905307.1"
                     /translation="MSTNRSNPGSLIAGAALIAVGLLALAGQLFRGFNFWGTIWPLFI
                     IGAGALFFVGMFSGGRSAAGLAIPGSILVAIGSMMFLQNLFDRWESWSYGWTVILIAV
                     GVGIYIMGRYTDNPGQRSSGLSLIKVGTILLVVFAGFFEMIFNDFAFSRFIFPAALIL
                     LGIYLIFGRSRSLTKA"
     gene            complement(743..1639)
                     /locus_tag="VFQ23_01665"
     CDS             complement(743..1639)
                     /locus_tag="VFQ23_01665"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HET9905308.1"
                     /translation="MDNILIVGTGALATLFAARLSQAGYSVSLLGTWKEGIDALRTNG
                     ARLIDASGTEHQFEVQASDDPGEYVGTRLALVLVKAWQTERAAAQLRECLADDGLAIT
                     LQNGLGNYETLVKALGGNRVALGTITIGATLLGPGLVKAAGDGNISMERKQMLDPIEA
                     ALRSAKFNVQIVEDARSILWGKLVINAAINPLTALLRVPNGELLKRPSAQDLMEALAC
                     EVAEVAKAEGIELPFNDPVVAAEDVARKTAVNRSSMLQDVLRNAPTEIDAICGAVVEA
                     AQRHGIDVPANWACWNLIKAIS"
     gene            complement(1687..2574)
                     /gene="panB"
                     /locus_tag="VFQ23_01670"
     CDS             complement(1687..2574)
                     /gene="panB"
                     /locus_tag="VFQ23_01670"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9905309.1"
                     /translation="MSATPALASTSAPVMRKKVTTLTYRQKKERGEIISMLTAYDYPT
                     AIAMDRAGVDSILVGDSLGMVVLGYENTLPVTMDEMLHHCRAVARGAKTALLIGDMPF
                     MSYQVSVEEATRNAGRFLQYGGMDAVKLEGGRERADAIRSITNAGIPVMGHLGLTPQS
                     VNQLGGFRAQGKTAAAAKRLVEDALILEEAGCFSVVLESVPERLAELISKKISIPTIG
                     IGAGAGCDGQVLVTHDLLGLFDRFTPKFVKKYANFHNEMQKAFADFIEDVETKRFPAP
                     EHTVEMKDEEWEMLLKEIG"
BASE COUNT          681 a          767 c          668 g          660 t
ORIGIN      
        1 gagcttttgt cagtgaacgc gaacgcccaa agatcagata aatacccaaa aggatcaacg
       61 ccgcagggaa gatgaatctt gaaaaagcaa agtcattgaa gatcatctca aagaaacctg
      121 caaagaccac caacagaatt gtgccgactt tgatcaggct taacccactt gaacgttgac
      181 cggggttgtc cgtatagcgg cccatgatgt aaatacccac accaacagcg atgaggatca
      241 cagtccagcc atacgaccag ctttcccagc gatcaaacag gttttgcagg aacatcatcg
      301 agccaatcgc gaccagaatg gacccaggaa tggccagacc cgctgctgat cttccgccgc
      361 tgaacattcc cacaaaaaac aatgcgcccg caccaatgat gaacaacggc cagatcgtgc
      421 cccagaaatt gaaccctctg aagagctgac ctgccagtgc gagcaagccg acagcgatca
      481 aagccgcgcc ggcaatgagc gaaccgggat ttgaacgatt cgtagacata attcttctcc
      541 ttcttccgct tatctcaaaa ctaaataaac tattgttcgg ctttgcaagc cggatccaat
      601 cctaacgtca cttaattacg cggcactctg caatttgagt ggcacttagg tatacgtggg
      661 ttgtgtacaa aggttgcccc tcggaagaag ggcttttatt tatcaggcga actgctcaat
      721 aaaaattacc cctcaaacta tcttaagata tggcttttat caaattccaa caggcccaat
      781 ttgcgggtac gtcgatgcca tgtctttgtg cggcctccac gaccgcacca cagatcgcat
      841 ctatctctgt cggtgcattg cgcagcacgt cttgcagcat ggacgaacga tttacagcag
      901 tcttacgcgc cacgtcctct gccgcgacaa ccggatcgtt aaatggcagc tcaataccct
      961 ccgcttttgc aacttccgcg acctcacaag ccagagcttc catgaggtct tgagcggatg
     1021 gccgtttcaa cagctcaccg tttgggacac gcagtaacgc ggtcaatggg ttgatcgcgg
     1081 cgttgatgac cagctttccc cacaggatcg atcgggcatc ttcaacaatt tggacattga
     1141 actttgcgga tcttaacgcc gcctcaattg ggtctagcat ctgcttccgt tccatggata
     1201 tattcccatc gccagcagct ttcactaacc cgggcccaag aagtgtggca cctatcgtga
     1261 tcgttcccaa tgccacgcga ttgccgccta aggctttaac cagcgtttca taatttccaa
     1321 ggccgttttg cagagtaatg gcaagtccgt catctgcaag gcattctctt aattgtgccg
     1381 cagcgcgttc cgtttgccag gctttgacga gaacgagagc aagtctcgtt ccaacatatt
     1441 cacctggatc atcactcgcc tggacctcaa attgatgctc agtgccactc gcgtcaatta
     1501 gcctggcgcc gttcgttcgt aacgcatcta ttccctcttt ccaggtgccc agcaggctga
     1561 ccgaatatcc tgcctgtgag agccgcgccg caaaaagcgt cgcgagcgcg cctgtgccga
     1621 cgatgaggat gttgtccata taagttgagg tgactgtcaa ttacaaattg acagtcacct
     1681 tgttgtttac ccgatttcct tcaataacat ttcccactct tcatctttca tctcaacggt
     1741 atgctcgggt gcggggaagc gtttggtctc tacatcctcg atgaaatcag caaaagcctt
     1801 ttgcatctcg ttgtggaagt ttgcatactt cttgacgaat ttgggtgtaa agcggtcgaa
     1861 tagaccaagc aagtcatgtg tgaccaaaac ctgaccatca cagcctgcac ctgcgccgat
     1921 accaatggtc gggatggaaa ttttcttcga gatcaattct gccagccgtt cgggaaccga
     1981 ttcaaggacc acgctgaaac agcccgcctc ttcaaggatg agcgcatctt ccacgagtcg
     2041 ttttgctgcg gcggcagtct taccttgtgc acggaatccg ccgagctggt tgacggactg
     2101 cggggtgaga ccgagatgtc ccataacagg aatcccggcg ttggtgatcg agcggatcgc
     2161 atccgcgcgt tcgcgtccgc cttcgagttt gacggcgtcc ataccgccgt attgaaggaa
     2221 gcgacctgca ttccgtgtgg cttcctcaac cgaaacctgg tacgacatga acggcatgtc
     2281 accgatgaga agtgccgttt tcgcgccgcg cgccaccgcc cggcagtgat ggagcatctc
     2341 gtccatcgtg acagggagtg tgttctcgta accgagtaca accatcccca gtgaatcgcc
     2401 aaccaggatg gaatcgacac ccgctctgtc cattgcaatg gcagtggggt agtcgtaggc
     2461 agtgagcatg ctgatgatct caccgcgctc cttcttctga cgataggtaa gtgtagttac
     2521 cttcttacgc ataacaggcg cgcttgtgga tgctaaagcg ggtgttgcag acatggtatc
     2581 ctccgaatag gtaggagccg tttggtgtgg cagtccctct gtgtgccaca attggacagc
     2641 ttgaataaga tgccgtcgcg ttcacgcaaa gcggatacgc gcggtgcttc tattattcca
     2701 caaaactcga aattcatcta taataaattt atcaaaataa tgttattgca catcgcgctg
     2761 agcgaagctc ccgaag
//
LOCUS       DASTJP010000055        14567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_380986, whole genome shotgun sequence.
ACCESSION   DASTJP010000055 DASTJP010000000
VERSION     DASTJP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380986"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1022)
                     /locus_tag="VFQ23_01675"
     CDS             complement(168..1022)
                     /locus_tag="VFQ23_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET9905310.1"
                     /translation="MSTKKKLYERFPISRRIEHWILVLSFITLAGTGLPQKYPNSPVS
                     VAIVRALGGVENLYDIHHFMSVIVMLAVIYHLLTIAYHVFVLRSKWSMMISLQDIKDA
                     LQVLLYNLRISKSAPKMGRYTFDEKFEYWAFVWGYSIMGITGFIMWNPITATRYLPGE
                     FVPAAKAAHGGEAVLAVLAILVWHMYHVGIRRSAKLMVTGMQTEEEMQDEHPLELEEI
                     KSGVANRRPDAQTIRKRQLVFFPVAALMTILMLTGVYRYITAEETALTTISPISTTAE
                     SPIQNPQP"
     gene            complement(1019..2182)
                     /locus_tag="VFQ23_01680"
     CDS             complement(1019..2182)
                     /locus_tag="VFQ23_01680"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET9905311.1"
                     /translation="MRVSKQLHPPPFWTVGLFLGFALIFFALTSLVRPTQAAPVLYQN
                     DQPSNDFCLACHQQEGMTLTVGSESLPVTINPTLFGLSVHAEEGLRCVDCHTNISEYP
                     HPDVQVPSIQDLSVALNGSCQDCHQEQFSNVQDSIHQRAMDEGNQNAPLCSDCHNPHT
                     QGRIIGTESKQLTISARLHVPETCAQCHSDKYDQYKDSVHGKALTEEGNVDVPTCTDC
                     HGVHNIQDPTTATVRNNTPQLCADCHDNAEIMDKYGISTNVLETYVADFHGTNVKLFG
                     DESPDEPTNKAVCTDCHGVHDITRVSDPQAGIALRENLLIKCQQCHPDATANFPGAWM
                     SHYVPSPEHYPIVYYVNLFYRIMIPTIIGGMIFFILTDIYRRIVSRGKGVQHS"
     gene            complement(2315..4495)
                     /locus_tag="VFQ23_01685"
     CDS             complement(2315..4495)
                     /locus_tag="VFQ23_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905312.1"
                     /translation="MSLKKVFIVTGLLLVSAALLMACGGNQVTETPSPTGEPTQAPVV
                     PDTPYLVDWQSSAHAAVDTEPFRHWDTEDPAEVPATCARCHTSTGYQDHIGADGTEAG
                     SVEAAVPATDSQGIQCVACHNQVTFSKTTVTFPSGVTITAGDDTRCMECHQGRESKVS
                     VDAQIERFGVTDLDAVVEPIQDDQGETVNFGFRNIHYYAAAATLYGGKTLGGYQYDGL
                     AYDSKNTHVRGYDSCVGCHDPHTLQVRVNDCAGCHQNVATVDDLKSIRMVSSAHDYDG
                     DGNVQEGMFYEIQGLQETLYAEIQKYASDKTDSGIVYDPAAFPYWFADANGDGAADQS
                     DSGAARFSTWTARLLKAAYNYQVSQKDPGAFAHGNKYIVQLLYDSIADLGGDTSQLAR
                     TDAAHFAGNTEPFRHWDAEEEGNGVVPFGCVKCHTATGLPAFVSTGGTTVVTSGGTTA
                     IVGLGSMPAGNGFMCTTCHTDPGGGERYTVNSVIFPSGKTVSFGGTDVDGNFLPDDSN
                     LCISCHQGRESTTSMNNALRGREPDTVDERIRFKNIHYFAAGATLFGADVQGAYLYEG
                     KEYLGQNMHADPDGRMNKCQDCHDVHKLEPKVEACETCHGTTDPTTIRRSELDYDGDG
                     DVTEGIHGEIETLGEALIAAMQSYAEAHGGAITYDPQAYPYFFDSNGEGYAAWTPRLV
                     RAAFNYQYIQKDPGAYVHNPKFVMQFLIDSIEDLGGDVSGYIRP"
     gene            complement(4978..5250)
                     /locus_tag="VFQ23_01690"
     CDS             complement(4978..5250)
                     /locus_tag="VFQ23_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HET9905313.1"
                     /translation="MSIIKARYQVPLELQSCHTAIVDGYVIEGHVPFQEIDRVLSERP
                     DIVGLAVPGMPVGSPGMENPGVAAEPYHVIAFYRDGRQEVFASYPR"
     gene            complement(5452..6222)
                     /gene="rquA"
                     /locus_tag="VFQ23_01695"
     CDS             complement(5452..6222)
                     /gene="rquA"
                     /locus_tag="VFQ23_01695"
                     /inference="COORDINATES: protein motif:HMM:NF038261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodoquinone biosynthesis methyltransferase
                     RquA"
                     /protein_id="HET9905314.1"
                     /translation="MSYTTTPINKQSSFIRPQIDSQSTEEGYGFDQGVSVPAYLESKY
                     WWAYVRPYAISFFDHEWIVNLILLGNMAKLRDTALDALGSSIHGRTLQIACVYGDFSP
                     RLIERLQPGSSLDIVDVVPAQLQNVNRKLSASAPVRLFQRDSVSLMKIEDAAYDQVVL
                     FFLLHEMPADVRERTLSEALRVLKPGGKLVIVDFHKPTAWNPFRYLYPPMFRLLEPFA
                     LDIWNEPIKDWLPKDFIPTQITKQTLFGGLYQKLVIYK"
     gene            complement(6542..6940)
                     /locus_tag="VFQ23_01700"
     CDS             complement(6542..6940)
                     /locus_tag="VFQ23_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9905315.1"
                     /translation="MQITRQADYATRAILFLARNQKRRHITTSQVAKQQNIPSSFLAK
                     IISQLSVAGLLYTSRGANGGVSLARDPKQITMLQVIEAIDGPIQLNVCVEQQNACILE
                     KNCPMKSVWHDAQNDLITKLKNTNFADLVA"
     gene            complement(6942..7142)
                     /locus_tag="VFQ23_01705"
     CDS             complement(6942..7142)
                     /locus_tag="VFQ23_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905316.1"
                     /translation="MNRKMNNKWKWIVSIAKAMIILLVPLLVLALVFQDSELLGMKTL
                     FPAWLVMIAWAALIVFGFSEEG"
     gene            complement(7259..7522)
                     /locus_tag="VFQ23_01710"
     CDS             complement(7259..7522)
                     /locus_tag="VFQ23_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905317.1"
                     /translation="MNNKWKWTVGTALAVILLALLLLSVQGFFPRGAYHMMNGYSWQM
                     PMMYGGGMMGFGMAFLMWLILFGVLILIGLGIAWLVKALTITK"
     gene            complement(7556..7981)
                     /locus_tag="VFQ23_01715"
     CDS             complement(7556..7981)
                     /locus_tag="VFQ23_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9905318.1"
                     /translation="MNTQTVRNWMTANPITITPQTTLHEARQLMDEHYIRRLPVLHKG
                     RLVGIVTRGDLREAQASTATTLNVYELNYLLDQLPAKEFMAYEPITVSADAPIGEAAR
                     RMQQHKIGALPVVEYGELVGIITESDLCRLVILQAETIA"
     gene            complement(8542..9198)
                     /locus_tag="VFQ23_01720"
     CDS             complement(8542..9198)
                     /locus_tag="VFQ23_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9905319.1"
                     /translation="MLKIMLVDDHEFVRMGINALLGRYPQFQVVAEAASAHEAVKQAI
                     QHRPDVIIMDIRLPGKSGIEATREITQQLPDTKVIMLTSFAEDDLLFEAIIAGATGYI
                     LKQIDSKELIRALETVARGEALLDPAVTKKVFERVRHAARASEDMMFASLSDQELRIL
                     ALVAQGKTNRDIAENLFLSEKTVRNYVSSILAKLNLSTRSEAAAYAVKHHIDDHLPTQ
                     "
     gene            9282..10637
                     /locus_tag="VFQ23_01725"
     CDS             9282..10637
                     /locus_tag="VFQ23_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9905320.1"
                     /translation="MNAYKLKIPVILLSILFIGAVLLGFAAIHPTTSWKEVLPVFSIL
                     LVLALTFIFWLFRLMEQHESETRLRSEQMAGLHKAALTLTREIELATVLQSVVDEART
                     LVVAQYGALGVLEEEGNYIDQFIVSGITQQQRALMGPPPRGHGLLGALIRTGEPIRVP
                     KISDDPRSVGFPPNHPPMRSLLGVPISFQGEILGDLYVTDKVGAAGKVTAFSEQDQQL
                     LEMFATQAAIAIKNAQFHRQSQQLTLLKERDRFGMDLHDGVIQSIYGIGLMLEEAQNR
                     FDSEAQGGKELVSQATDGLRSTIRDIRNYIMDLRPQQFQGRDLREGLAELARELRAHS
                     FLGIDIQVETDDLMRLSPEQTVEILHMSREALTNVRKHARASQVDIHVTSQDDVLQIS
                     ISDNGIGLDPNQLTTETGNGLHNMQDRAQGLGGNVMFSKRESGGTRVQFEVPFSSPTL
                     S"
     gene            10786..11199
                     /locus_tag="VFQ23_01730"
     CDS             10786..11199
                     /locus_tag="VFQ23_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9905321.1"
                     /translation="MLLKEEVQPRKIRILLADDHSIVRKQLAIRLSSEPDLEIVEVAS
                     NSRETFQKAQATHPHILLIDPLMRDGLGVATLRQVRVNFPEIIIVVLTAYVDTMLKMH
                     IQRMGVRHILTKGIVSSELVAELREAASTRSSDRL"
     gene            11292..11365
                     /locus_tag="VFQ23_01735"
     tRNA            11292..11365
                     /locus_tag="VFQ23_01735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11326..11328,aa:Arg,seq:tcg)
     gene            complement(11427..11603)
                     /locus_tag="VFQ23_01740"
     CDS             complement(11427..11603)
                     /locus_tag="VFQ23_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905322.1"
                     /translation="MKTKPPVNDRGLINQITLTCKLIQVREKESPGLSGIWNDEQDDE
                     YVRSWYSLFGSIPM"
     gene            11624..12634
                     /gene="hisG"
                     /locus_tag="VFQ23_01745"
     CDS             11624..12634
                     /gene="hisG"
                     /locus_tag="VFQ23_01745"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET9905323.1"
                     /translation="MQLQSIRLSLPSKGRLETSALDFLAAAGLRVHKPNPRQYVADIP
                     ALPELGVIFQRPGDTVVSVRQGSVEFGIAGLDVIEEKRGTNGDILILHDALGFGHCTL
                     RLAVPEAWDDVQDVPSLKQFASKLNHPLRVATKFPVLTERFLRQYDIEHILIAAEGTL
                     ETAPTIGYADIISDLVSSGQTLQDNRLRPLPDGIIQTSQAALIANREILQSRPEVLEM
                     ARRLLEYIEAHLRAKENLLVIANMRGDNPEMIAQRMFAQSIIGGLQGPTISPVIVREG
                     EVRWYSASVVVRRDLLPQAITELRSIGGSGIIVSPITYIFEEEPPRYTAMLNALEQKN
                     YA"
     gene            12627..13973
                     /gene="hisD"
                     /locus_tag="VFQ23_01750"
     CDS             12627..13973
                     /gene="hisD"
                     /locus_tag="VFQ23_01750"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET9905324.1"
                     /translation="MLRQYDAPTARGTILRRVPLDEVTVSKPMMERIEKLFGEALTPE
                     QAVSAILMDVRTKGDLALQKWTKTLDQIDVAPAPVSKALIQSALDSLEPAQRAALEQA
                     AARIEAFHRRQPIHSWFTNELGGTLGQMIRPIQRVGLYIPGGTAPLPSTVLMSAIPAR
                     VAGVKEIVVVTPPGRLLTEKNPPINPMILAACAIAGVDEVYPLGGAQAIGALAYGTQT
                     IRAVDKIFGPGNLFVTLAKRQVYGLVGIDGLAGPTETVVIADEFANPAWVAADLLAQA
                     EHDILASAILLTPSQTLIEHVQVEYSRQIEQRARADVILTSLENCGGAVLTHDLEEAI
                     QLANDYAPEHLALSVKDPWRWVEKVSNAGGIFLGEHSFEVLGDYLAGPSHVMPTGGSA
                     RFASPLNVFDFVKIISLIALDEKTALEISPLTATIAECEGLDAHEYAALVRSGDTS"
     gene            13970..>14567
                     /locus_tag="VFQ23_01755"
     CDS             13970..>14567
                     /locus_tag="VFQ23_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9905325.1"
                     /translation="MKIRAHLNSLPPYTPIEPFEVLSTRLGRLPEQIIKLDANENPYG
                     PLPEVRVALGKLDFPHIYPDPESRALRKSLAEFTGIPTEYLLAGAGADELIDLLMRVI
                     LEPGDSVLSCPPTFGMYSFDAELNAARVIEVPRRADFSLDMASIRKAIETHQPRILFL
                     ATPNNPDGSMPDAKLIDELLELPLLVVLDEAYIEFADSQ"
BASE COUNT         3624 a         3620 c         3602 g         3721 t
ORIGIN      
        1 aaacccgccg caaaattctc tgaaacccca tctggttttt ctgcagattg atcctcttgc
       61 caaccagcag ggtcatgacc agaaggcacc aataaaaaca tcttaaggga acttcgagat
      121 cggctagtat ttagccctga aggaatgttc tctgtgatgg ttatgcccta cggctgtgga
      181 ttttgtatgg ggctctctgc cgtggtcgaa attggcgaaa tggtcgtcag ggcagtttcc
      241 tctgctgtaa tatatcgata tacgcccgtc agcatcaaga ttgtcatcag tgcggcaaca
      301 gggaagaaga ccaattgtcg tttgcggatc gtttgggcat ctggtctccg attggcgaca
      361 cctgatttaa tttcctccag ctcgagcggg tgctcatcct gcatttcctc ttctgtttgc
      421 atgccagtga ccatcaattt ggcactgcgc ctgatcccca cgtgatacat gtgccagacc
      481 agaatggcga ggacagccag gactgcttct ccgccatgtg ccgctttggc ggctggtaca
      541 aattcgccag gcagatatct tgtggctgta attggattcc acatgatgaa gcccgtaatg
      601 cccatgatgc tatatcccca tacgaacgcc cagtattcga atttttcatc aaaagtgtag
      661 cgtcccattt tgggggcaga ttttgaaatg cgaagattat agagaagaac ctgcagggca
      721 tcctttatat cctgcagcga gatcatcatg gaccatttcg aacgtagtac aaacacgtgg
      781 taggcaatcg tcaacaaatg atagatcacc gccagcatga cgattacaga catgaaatga
      841 tgaatatcgt agagattctc aacaccgcct aatgctcgca cgatcgccac acttaccggc
      901 gagttcggat atttttgcgg cagtcccgtc ccggccagtg tgatgaatga taagaccaga
      961 atccaatgct caatacgacg cgagattgga aaacgttcat agagcttttt tttggtactc
     1021 atgaatgctg gactcctttt ccacgactga cgattcgcct gtaaatatca gtgagaatga
     1081 agaagatcat accgccgata atggtaggga tcatgatcct ataaaataaa tttacgtaat
     1141 agacaatggg atagtgttcg gggcttggta catagtgact catccaggcg ccagggaaat
     1201 tcgcagtggc atccgggtgg cattgctggc atttgatcag caggttttcg cgcagggcaa
     1261 tgccggcttg cggatcgctg actctggtaa tgtcatgcac gccatgacaa tctgtgcaga
     1321 ccgctttgtt cgtgggctca tcgggggatt catcaccaaa gagttttaca tttgtgccgt
     1381 ggaaatctgc cacatacgtt tccagtacat tggtcgagat cccatattta tccatgatct
     1441 cagcattatc atgacagtct gcacagagtt gcggcgtatt gtttcggacc gttgcggtgg
     1501 ttgggtcttg aatgttatgc acaccgtgac agtctgtgca ggtgggaaca tccacattgc
     1561 cctcttctgt cagtgccttg ccatgtacac tatctttata ctgatcatat ttgtcactgt
     1621 gacattgtgc acaggtttcg ggaacatgca atcgtgcgga gatagtcaac tgcttgctct
     1681 ctgtgccgat gatgcggccc tgtgtatgcg ggttatgaca atcggaacac agtggagcat
     1741 tctggttgcc ctcatccata gcgcgttgat ggatgctatc ctgaacattt gaaaattgtt
     1801 cctggtgaca gtcctggcag gaaccattca gcgccacgga gagatcctga atgctgggca
     1861 cttgtacgtc cggatgagga tactcgctga tgtttgtatg acaatccaca cagcggagtc
     1921 cttcttcggc atgaactgaa agcccgaata aggttgggtt gatcgtgact ggaagagatt
     1981 cacttccgac tgttaatgtc atgccctcct gttggtgaca agccaggcag aaatcgttgg
     2041 agggctggtc attttgatat aggacgggag cggcctgtgt aggacgaacc agggaggtta
     2101 gggcaaagaa aatcaatgcg aatccaagaa atagtcccac ggtccagaaa ggaggcggat
     2161 gtagttgttt gctgactctc attgaagtct cctcaagcac cactcaaaat ctaaaattct
     2221 cgatttgtct tcttatcaaa atttctaacg ttgcctaagc aagaaaagat ggcagacgtt
     2281 cggtctacca tcttctttgt tgtaaagtct gcgcctacgg ccgaatgtat ccgctcacat
     2341 cgccgccaag atcctcgata gagtcgatca ggaactgcat cacaaatttg ggattgtgaa
     2401 cataggctcc ggggtctttc tggatgtatt gatagttaaa ggcggctctc acgaggcgag
     2461 gcgtccaggc ggcatagcct tcgccatttg agtcgaaaaa gtacggatag gcctgcgggt
     2521 cgtacgtgat ggctccacca tgcgcctcgg catacgattg catggcggca atgagcgctt
     2581 cgccaagtgt ttcaatttca ccgtgaatac cttctgtcac atcgccatca ccatcgtaat
     2641 ccaattcgct tctgcggatt gtcgtggggt cggtcgtgcc atgacaggtc tcacaggcct
     2701 ccaccttcgg ctcgagctta tgcacatcat gacaatcctg gcatttgttc atccttccgt
     2761 caggatcagc gtgcatgttt tgacccagat attccttgcc ctcataaaga tacgcgcctt
     2821 gaacatctgc tccgaagagt gtggctcccg ctgcaaagta atgaatgttt ttgaaacgga
     2881 ttctttcatc cactgtatct ggttctcttc ctcggagggc attattcatg ctggtggtgg
     2941 actcgcgtcc ctgatgacag gagatacata gattggaatc atctggaagg aagttgccat
     3001 ctacatctgt tccgccaaaa ctgacagtct taccgctggg gaagattact gaattgactg
     3061 tatagcgttc tccgcctccc ggatctgtat ggcaggtggt gcacatgaag ccattgccgg
     3121 caggcatcga tcccaaccca acaattgcag ttgttccgcc agacgttacc actgtggtac
     3181 cacctgttga aacgaaggcg ggcaagccag tcgcggtgtg acatttcacg catccaaacg
     3241 gaactactcc attgccttcc tcctccgcgt cccagtgacg gaacggctca gtgtttccgg
     3301 caaaatgggc cgcatctgtg cgtgcgagtt ggcttgtatc gcctccaagg tcggcaattg
     3361 agtcatacaa caattggaca atgtacttat taccgtgcgc gaacgcaccg ggatctttct
     3421 gtgatacctg gtagttgtag gcggctttta gcagacgcgc cgtccaggtg ctgaagcggg
     3481 ccgcgccgct atcgctttga tcggctgctc cgtctccgtt agcatctgcg aaccaatatg
     3541 gaaaagctgc cgggtcatag acaatccctg aatcggtttt atcagacgcg tacttctgga
     3601 tttcggcgta cagagtttcc tgcaggccct ggatctcgta gaacatgccc tcctgcacgt
     3661 tgccgtcacc atcataatcg tgcgcagaac tgaccatacg aatggatttg agatcatcaa
     3721 cggttgccac gttttgatga caacctgcac aatcatttac cctcacctgc aaggtatgcg
     3781 gatcatgaca acctacgcaa ctatcataac cccggacgtg cgtattcttg gaatcgtatg
     3841 caagcccgtc atattgataa ccacccagtg tcttaccacc atataaggtt gcagccgcag
     3901 cgtagtaatg tatgttgcgg aaaccaaaat ttacagtctc accttgatcg tcctggattg
     3961 gttcgaccac tgcatccaga tcagtgacac caaatcgttc gatctgcgcg tccacgctca
     4021 cttttgattc gcgcccctga tggcattcca tacaacgggt atcgtctccc gccgtgattg
     4081 tgacaccgga gggaaaagtg acagtggtct ttgagaatgt gacctgattg tggcatgcca
     4141 cacattggat cccctgtgag tccgtggcag ggacagctgc ttctacgcta cctgcttccg
     4201 tcccgtccgc gccgatatga tcctgataac cggtgctggt gtgacatcgt gcgcacgtag
     4261 caggaacctc cgccggatct tcagtatccc aatgtcgaaa tggctcagtg tccacggcag
     4321 catgagctga actttgccag tccacaagat aaggtgtatc tggcacaacg ggtgcttgtg
     4381 ttggttcccc ggtgggggat ggtgtttcag taacttgatt tcccccacat gccatcagga
     4441 gcgcggcact gaccagcagc aaacctgtga cgatgaaaac tttctttagc gacattttac
     4501 cttctcctta ctcgacaatg ttcgaaatag ggtgataaag tgagcttagg tttacgctaa
     4561 aaaggagact tgcagttagg aaaattaatt tggcgttaat aatatactcc aatcaaaaaa
     4621 aatgtcaagg tttttgtgaa caagccagtg acttcattga aacgccagca ttttccataa
     4681 ctgatcaacc agcagcatcc ctacaataac gaactcgatc aaccttggtg gtttgctcag
     4741 tatggggttg atctcccgaa ctggcacctt ggcgtgtaca ggtcatggaa gagattgcat
     4801 tcggtgggtg cgtgaaaatc tacgatgacc aatctgcagc caagcttacg ttaattgaaa
     4861 atgatgttaa atctcaagga ttgaccttgt ttcaatacta cagccgaacc gcaagaactg
     4921 tcaaggtttt tgtgaaaaaa aacacaatct ggacaatttg gaccaatttg ggcatattta
     4981 cctgggataa ctggcaaaaa cctcctgccg accatcacga taaaatgcaa taacgtgata
     5041 cggctcggct gccacaccag ggttttccat ccccggtgaa ccgaccggca ttccgggaac
     5101 agcgagccca acaatgtccg gccgttcgct caacacacgg tcaatctcct gaaacggaac
     5161 atgtccttca atgacatagc catccacgat ggcagtgtga catgactgga gttcaagtgg
     5221 gacctgataa cgagccttga taatactcat gtcgctcaca acctccacag cgatatcaaa
     5281 accgttattt tccagatatt gaatccagtt gtcacaacat cctcaggtag gcgacttgaa
     5341 gaccgtcata gaaatttttt gtgtattatt atctaccggg ccgcatgcgc tcaacaaaac
     5401 gatgccaagc aggatcagcc gaaaaaaacg ggtcatagtg actcctttcc tttacttgta
     5461 tatcaccagc ttttgatata gacccccaaa caatgtttgt ttggtaattt gagttggaat
     5521 gaaatcttta ggcagccagt ctttgatcgg ttcattccaa atatcaaggg cgaagggttc
     5581 cagcagacgg aacattggtg ggtaaaggta gcggaatgga ttccatgcgg taggtttgtg
     5641 gaaatccacg atgaccaatt taccgccggg cttgagcact cgcagcgcct cgctcaatgt
     5701 ccgttcacga acgtctgcag gcatctcatg caagaggaag aataggacaa cctgatcgta
     5761 tgcagcatct tcaattttca ttagagaaac ggaatctctt tgaaaaagcc gcactggcgc
     5821 agacgccgaa agtttccgat tcacattttg tagttgtgct ggcacaacat caacgatgtc
     5881 gagcgagcta cctggctgaa gtctctcaat caaccgcgga gaaaaatcgc catagacaca
     5941 tgcaatttgc agggttcgtc cgtgaatgga tgaaccaagg gcatccagtg cggtgtcgcg
     6001 aagctttgcc atgtttccca ggagaatgag attgacgatc cattcgtgat caaaaaagga
     6061 aattgcataa ggtcgcacat aggcccacca gtatttactt tccagatagg cgggtacgga
     6121 gacgccttga tcaaatccat atccttcctc agttgactgt gaatcgatct gcggacggat
     6181 aaaactgctt tgcttattga tgggagttgt tgtgtagctc atgatgttgc tttcctttca
     6241 gtccaaattt ttttattact taacatgtgt ttcatttcaa ccattttcgc ccttctttct
     6301 ccgatagtga tgtggcgagt atcccggttt tgttgggaca gatgacccgg aacggcactc
     6361 ccggtaggca ttccgaaaga tcaatatgcg gttgatgact aaaaaatgaa ctgcctatga
     6421 gggtggcagt tcattttttt ctcttcgaaa tgtcatttat ggacgtggaa agcaattttt
     6481 gatttgtaac tttcgcaccg cgcgatctcc ctgggtttgc tctcaaatcc tggatgaaaa
     6541 cttaagcaac cagatccgca aagttggtat tctttagttt cgttatcaga tcattttgcg
     6601 catcgtgcca aacggacttc atcggacagt tcttttcgag aatgcaagca ttttgctgtt
     6661 caacacaaac atttaattgg atcgggccat caattgcctc gatgacctga agcattgtga
     6721 tctgtttcgg gtcccgagcc aaagagactc ccccatttgc accccgggat gtataaagca
     6781 aacccgcgac ggaaagttgt gaaatgattt ttgccagaaa ggaggatgga atgttttgtt
     6841 gttttgcaac ctgactggta gtaatatgcc tgcgtttctg attgcgtgcc aaaaataaga
     6901 ttgcgcgcgt tgcataatca gcttgacggg tgatttgcat gttatccttc ctcagaaaat
     6961 ccaaacacaa ttaacgctgc ccaggcaatc atgaccagcc aggcagggaa taaagtcttc
     7021 atccctaata gctcgctgtc ttgaaagacc aaggctagaa ccaacagtgg gacgagcagg
     7081 atgatcatcg ccttggcaat gctcacgatc catttccatt tattattcat ctttctgttc
     7141 atttcatatt ccttccgatc cgttgggaag cttcataacg gatgcgctac aacagggaaa
     7201 tccgaagcga atagaagaca tgtttcctgc gcgaaacatt cgtgcaaccg ctgggaattt
     7261 atttcgtgat ggtcaaggct ttgaccagcc aggcgattcc cagcccgatc agaatcagta
     7321 cgccgaatag gatcagccac atgagaaacg ccatgccaaa acccatcatc cctccaccat
     7381 acatcatggg catctgccag ctatagccgt tcatcatgtg gtatgcgcca cgggggaaga
     7441 atccctgtac agacagcagt agaagcgcca gtaggatcac cgccagggcg gtaccgacag
     7501 tccacttcca tttgttattc atcctacgtt cctttctcta tatctccaat catttttacg
     7561 cgatagtttc cgcttgcaaa attaccagcc ggcagagatc ggattctgta atgatgccga
     7621 ccagttcgcc gtattcgacc acaggcaaag caccgatctt gtgttgctgc atccggcgtg
     7681 ccgcttcgcc aattggagcg tcagcagaaa ctgtgatagg ctcataggcc atgaattctt
     7741 ttgccggcaa ctggtccagc aagtagttca actcatacac attcagcgta gtagcggtag
     7801 aggcctgcgc ttcccgaaga tcgccccgcg tgacaatccc tacaagcctg cccttgtgca
     7861 agacgggcag acggcgaata tagtgttcat ccataagctg gcgagcttca tgcagtgtcg
     7921 tttgtggggt gatcgtaatg gggttggcag tcatccagtt tcgaacggtt tgagtgttca
     7981 tagccgtatt ccttttcgca cattgtcgct ctacaccctc ccgtcgtcat gtggcaagta
     8041 tcccggtttt ttcaggacag atgacctcta aattcacata ctctacgcta gatcaaggga
     8101 atcgcaattt tcagaatgta cagggatgaa aatacataaa ttgatgatga ggttcagcag
     8161 tgaggcgatg aaaacccttc tccgcacccg actgcatacg ttggtcggcc agaattcgct
     8221 gtttattacc gtaacaggat gtagtacttc cgcgcaggag tattcttgcc tgtgaattgc
     8281 ctacaggatg gcgctacgct ctacatcatt tggaaacaca gcagaacagg gtggcgaaat
     8341 ctgcgcggta gcatgccagt tggcgattgg cttcactgat atcaagttga tgcgtatgcg
     8401 attactgatg ataaatctgc aattgcattg gaaaacttaa aatctgtttc aaaccccacc
     8461 tgggaggtct cgatatttcg gcgtctgcct ggaaagcaat aaccaaccct tgggcagcgt
     8521 agagaatttg aaaggcttga gttactgtgt gggcaagtga tcgtcgatat gatgtttcac
     8581 tgcataagcg gccgcttcag aacgggtgga taagttgagc ttggcaagta tggaactcac
     8641 ataattacga accgtctttt cacttagaaa caaattttcc gcaatatctc ggttggtttt
     8701 cccctgggcc accaaagcca gtatgcgcaa ttcctgatcc gaaaggctgg caaacatcat
     8761 gtcctcgctc gctcgagccg cgtgccgaac tcgttcgaac acttttttag tcaccgcggg
     8821 atcaagaaga gcctctccac gcgcaaccgt ttcgagtgcg cgaatcaatt ccttgctatc
     8881 gatttgtttg agaatatagc cagttgcacc tgcgataatc gcttcaaaga gcaagtcgtc
     8941 ctcagcaaat gaggtcaaca taattacttt tgtgtcgggc aattgttggg tgatctctcg
     9001 cgtggcttcg atgccacttt tccccggcaa gcgaatatcc ataatgatga catcggggcg
     9061 gtgctggatg gcttgtttca ccgcttcgtg ggcgctagct gcttctgcca ctacttgaaa
     9121 ttgcgggtac ctgcccaata aagcattaat acccatccgc acaaattcat ggtcgtcgac
     9181 caacatgatt ttcaacatgt gagactaaaa tctccgtaga gtgaattaca gctataatcg
     9241 aattaagtca gctcaccagg acaaattata acatcttgaa gatgaacgcc tataaactga
     9301 aaataccagt cattctttta tccatactct tcataggggc agtgttatta gggtttgccg
     9361 cgatccatcc aacgacctcc tggaaagaag ttctgccggt ttttagcatc ctgcttgtat
     9421 tggcactgac attcattttt tggctgttcc gactgatgga gcagcatgaa tccgaaaccc
     9481 gtctgcgctc tgaacaaatg gcagggcttc ataaggcagc actaacgttg acccgggaaa
     9541 tcgagttagc gacggttctc caaagtgttg tcgatgaggc tcgaacactt gtggttgctc
     9601 agtatggcgc actgggcgtc ctggaagaag aaggtaatta cattgaccaa tttattgtgt
     9661 ctggcattac ccaacagcaa cgcgctttga tgggtccgcc accgcgaggt catggtctat
     9721 taggtgcgtt gataagaacc ggcgagccca tccgagtccc gaaaatctct gatgaccctc
     9781 gctcggttgg ttttcctccg aatcatcctc ccatgcgatc tttgttgggg gttcccatct
     9841 cgtttcaggg agaaatttta ggcgatcttt atgtaacgga taaggtggga gccgcaggaa
     9901 aagtaacggc cttttccgag caggatcaac agttattgga aatgtttgcg acacaagcgg
     9961 cgattgcgat caagaatgct caattccacc gccaatcgca acaacttacc ctccttaagg
    10021 aacgagatcg gtttggtatg gaccttcatg atggtgtcat acaatccatt tatggtatcg
    10081 gcctgatgtt ggaagaagca caaaaccggt ttgattctga ggctcaaggt gggaaggaat
    10141 tagtttcaca ggcaacagac ggactacgaa gtacgatccg cgatattcgc aactacatca
    10201 tggatttacg tccacagcaa tttcagggac gcgacctccg tgaagggttg gcagaactgg
    10261 cccgtgagct gcgcgcgcac tcatttttgg gtatcgatat acaggtcgag acagatgatt
    10321 tgatgcgact ctcgcccgaa caaaccgttg aaattctcca tatgtctcgc gaggctctga
    10381 cgaatgtaag aaagcatgcg cgcgcaagcc aggtagacat ccatgtgacg agtcaggatg
    10441 acgttttaca aatcagcatt agtgacaatg gcatcggcct ggacccaaac caactaacga
    10501 ctgaaaccgg aaatggccta cataatatgc aggaccgtgc ccagggattg ggtggcaacg
    10561 tgatgtttag taaacgcgag tccggcggaa cccgtgttca gttcgaagtt ccatttagct
    10621 cgccaactct ttcatagacg ttataaatag catttgcaca cgaggtaggc ggaagcgagt
    10681 ctggatgaca tcgtggtttt aagcacccgc acctgacatt tgctccatct atttcgggac
    10741 aattaacacg tgtgttgtct acgtcattgt gatacacatg gaggcatgtt attaaaagaa
    10801 gaggttcaac cgcgaaaaat ccgtatactg ttagccgacg atcattccat cgtccgtaaa
    10861 cagctggcga tacgattaag ctcagagcct gatctggaaa ttgtagaagt tgcatccaac
    10921 agtagagaaa cattccagaa agcccaggcg acgcatcccc atattctctt aatcgatccg
    10981 ttgatgcgcg acggattggg tgtggcaaca cttcgacagg tgcgcgtgaa ttttccggag
    11041 atcatcattg tagtcctgac cgcctatgta gatacgatgc tcaagatgca tatccaacgg
    11101 atgggggtac ggcacatctt gacgaaaggc atcgtttcct ccgaactggt cgcagaattg
    11161 cgcgaagccg cctccactcg ttcttcagac cgcctctaaa atctagcagc cgggtttcaa
    11221 gcgttgaaag ttcaacatcc cagcgccaga cagtgggttt gtttttttaa aggtataatt
    11281 acggctcgaa agcccctgta gctcaactgg atagagcgtc tgacttcgga tcagaaggtt
    11341 gtgggttcga gccctgccag gggcgctctt atataataat gcggtgagcg agttcaagag
    11401 caaatctcgt gcttgtcaaa taaccttcac attggtatac tgccgaacaa tgaataccag
    11461 ctacgcacat attcatcatc ctgttcatca ttccaaatcc ccgaaagacc gggcgattcc
    11521 ttttcgcgta cctggataag tttacaggtt aacgtaattt ggttgatcaa cccccggtca
    11581 ttgaccgggg gttttgtttt caattttaca gaaaggactc atcatgcaat tacaaagtat
    11641 tcgactttcc cttccatcca aaggcaggct tgaaaccagc gccctggact ttcttgctgc
    11701 agccgggctt cgtgtgcaca agccaaatcc gcgtcagtat gtggcagata tcccggctct
    11761 acctgaattg ggggtgatct tccagcgccc tggcgatact gtagttagtg tgcgtcaggg
    11821 cagcgttgaa tttggcatcg ccggattgga cgtaatcgag gaaaagcgcg gcaccaatgg
    11881 agacatcctc attctgcacg atgctctcgg tttcggacat tgcacgctgc ggctggccgt
    11941 ccctgaagca tgggatgatg ttcaggatgt tccctccctc aaacaattcg cgagcaagct
    12001 caatcatcca ttgcgtgtgg caacaaagtt ccccgttctt acagaacgct ttctacgcca
    12061 atatgatatt gagcacatat tgatcgcggc agagggaaca ctggagaccg cgccaacaat
    12121 tggatacgcg gacataatct cagacctggt ttcctccggt cagacgctgc aggacaatcg
    12181 cctgcgccca ctgcccgatg gaattatcca gacgtcgcag gcggcgttga tcgcgaatcg
    12241 tgagatattg cagagccgcc cagaggtact tgagatggcg cgtcgcctgc tggaatacat
    12301 cgaagcacac ttgcgggcca aagagaacct gctggtcatt gccaatatgc gaggcgataa
    12361 cccggagatg atcgcccaaa ggatgttcgc gcaatccatc attggcgggc tgcaggggcc
    12421 cacgatcagc cccgtcattg tgcgcgaagg ggaggtcagg tggtactccg cttctgtggt
    12481 cgtgcgccgc gatctgcttc cgcaagccat cacagaattg cgctctattg gcggcagcgg
    12541 tatcatcgtt tctccgatca cgtatatttt cgaggaagag ccgccgcgct acaccgccat
    12601 gctcaatgcg ttggagcaga aaaattatgc ttaggcaata tgacgcaccg accgccagag
    12661 gaacaattct tagacgcgtc cctctggatg aagtcacagt cagcaagccg atgatggaac
    12721 gaatcgagaa acttttcggg gaggcattaa ctcctgagca ggccgtttca gcgatcctga
    12781 tggacgtacg tacgaagggt gaccttgcgc ttcagaagtg gacgaagacc ctcgatcaaa
    12841 tcgacgtcgc gcctgctccc gtttcaaagg cattgatcca atctgcattg gactctctcg
    12901 agccggcaca gcgcgcggcg ttggagcaag cggccgcacg gatcgaagcc ttccaccgaa
    12961 ggcaaccaat tcattcatgg tttacgaatg aactgggtgg cacgctgggg cagatgattc
    13021 gacctattca acgcgtcggg ttgtatatcc ccggaggaac cgcgccactt ccatcgactg
    13081 tgctgatgag tgccatcccg gcgcgcgtgg ctggtgtaaa agaaatcgtt gttgtgactc
    13141 ctccaggacg cctactgact gaaaaaaatc ctccgatcaa tcctatgatt cttgccgctt
    13201 gtgcgattgc cggagtggat gaggtgtatc cgcttggcgg cgcgcaggcg atcggcgcat
    13261 tggcatacgg cactcaaacg attcgagccg tagacaaaat attcgggccc ggaaaccttt
    13321 ttgtcacatt ggcaaaacga caagtctatg gattggttgg gatcgatgga ctggcaggtc
    13381 cgacggaaac cgttgtcatc gccgatgagt ttgccaaccc cgcctgggtt gctgccgact
    13441 tgcttgcgca ggcagagcat gacatcctgg cttcagcaat tttattaact ccgtcacaga
    13501 ctctcattga acatgtgcag gttgaatatt cacggcagat cgaacaacgc gcgcgtgcag
    13561 acgtaattct tacttccctg gaaaactgcg ggggcgcagt cctgacgcat gatctcgaag
    13621 aagcaatcca attagcgaat gactatgctc ctgaacacct cgcgctctcg gtgaaagatc
    13681 cttggcgctg ggtggaaaaa gtaagcaatg caggaggcat cttcctgggt gaacattcct
    13741 ttgaagtcct tggcgattac ctcgccggac ccagtcacgt gatgccgact ggcggctctg
    13801 cgcgttttgc ctctccgttg aacgtgttcg attttgtgaa aataatcagc ctgatcgctt
    13861 tggatgaaaa gacagctctg gagatcagtc ctcttacagc gacgattgca gaatgcgaag
    13921 ggttggacgc acatgaatat gctgcgctgg ttcgttcggg agatacttca tgaaaattcg
    13981 cgctcacctt aattcgttac caccttatac accgattgaa ccgttcgaag tgctatcgac
    14041 gcggttgggc cggctgccag agcagatcat caagctggat gcaaatgaaa acccctatgg
    14101 accgctaccg gaagtgcgag tggcactggg gaaattggat tttccacaca tctatccgga
    14161 ccctgagagc cgcgcgctaa gaaaatctct cgcagaattc acaggtatac ccaccgagta
    14221 tttacttgcg ggagcgggcg ccgatgagct aattgatctg ctcatgcgag tgatccttga
    14281 gcctggtgat tctgtgcttt cctgcccgcc gacctttggg atgtactcct tcgatgcgga
    14341 actgaatgcc gcgcgcgtga tcgaggtacc tcgccgcgca gacttttcac tggatatggc
    14401 ttcgatccgt aaagcaatcg aaacccacca gcccaggatt cttttccttg cgacacccaa
    14461 taacccggat ggctcaatgc cagatgccaa attaatcgat gaactgttgg agcttccact
    14521 tttggtggtc cttgatgaag cctatattga gtttgcggat agccaat
//
LOCUS       DASTJP010000056         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_76303, whole genome shotgun sequence.
ACCESSION   DASTJP010000056 DASTJP010000000
VERSION     DASTJP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76303"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFQ23_01760"
     CDS             <1..267
                     /locus_tag="VFQ23_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9905326.1"
                     /translation="WEAINEKTPHAIILDIFMPEMDGFTILENLREHPTLRDVPVLVV
                     SGGGLTNEQQQQLNEYGQRLIMKGSLSEEELLSSIEAALKKIGA"
     gene            428..2104
                     /locus_tag="VFQ23_01765"
     CDS             428..2104
                     /locus_tag="VFQ23_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9905327.1"
                     /translation="MSFVIRCLDCGYAAPFNPSSMHCPQCNSQWREAEYDLVEVAKTF
                     PSELKKRPFDLWRYSELLPIRNPNPTLRLGEGGTPLIQAANLGMMLGLPNLFIKDERQ
                     GPTSSFKDRQAAVTIAALKEGGVTEMVAASTGNVAIALSAYAARAGIKLWAFVTSLVP
                     GVKMREIALYGSQVVKITGSYDQAKQVAAEFARQRGLYQDMGARTVTAVEAMKTLAFE
                     IAEQLTLAQGFTTGSTSETPKWRTPDWYIQAVSGGMGPLGVYKGFRELKQLGLINRIP
                     ALVPIQAEGCAPMVESWKKGLEIAEPVLSPQTRIETLATGDPGRTYTMLRNQVNETGG
                     VFESVSDEDSFRAMHVLAKMEGISAEPAAGTAFAGLFKLVRAGVIKPTDTVVVNCTGH
                     TLPAEQFLFGENWTRDIDLLPRAEQSPTPQEGLLSALNNVTPNRFSRVAVVDDSPEAR
                     RLIRRILQSQGDFEIFEAIDGREAIELVTRERPDLVILDLMMPEVDGFAVLDALRSQP
                     DTANIPVIVATAKELTVDEKARLQGQIQSLMQKGDFLNDEFLEEVRSLIR"
     gene            2190..3143
                     /locus_tag="VFQ23_01770"
     CDS             2190..3143
                     /locus_tag="VFQ23_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9905328.1"
                     /translation="MSRMQNGRLAGINAALISAFFLGLAPVFGKAAMGGDHFSPFAVV
                     ALRTSAAALLLFLIMAIFNRKFLYIYGAGLLGCVIAGSINGIGSLFYYVGLSKLNASI
                     AQMLYSLYPFFVATWLILDRQPLSRLTTFRIAIALVSAILLTQSGAGTVDPIGVACMI
                     IAAGFYAAHIPINQRVLYDVPPPTVTLYTLISMSVIVVGAYFIFDRSQPTGEAPWIPV
                     FALTMVTFFSRLTLFLGIKHIGGMQTALLGLGELIVAILFSHLLLNETLSNVQWVGVA
                     CLGISLLMVLFEKPLPHPPVHPHGLLSWLQPPDFPEDFYKH"
     gene            complement(3198..3932)
                     /locus_tag="VFQ23_01775"
     CDS             complement(3198..3932)
                     /locus_tag="VFQ23_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9905329.1"
                     /translation="MKSNILVVDDEPVARQSLSDILRLEGYIVNAVPNGQAAVEYVRT
                     HPVELMVVDLRMPGMDGLEVVQVVNQVSPETEVILLTAFGTTESAIQALRLRIHDYLL
                     KPVPPAQVVNSVKKGLTRREARLKMRGGTASIDVDEGMAEFSLKDGTHIDLSRRQVRK
                     KDQIIHLTPAEGRLLRVLIENPGRVYSHRELVLLVQGYDTSQREAPEILRPLVSRLRH
                     KLDAFPALSDRIVSVRGTGYLYEGDK"
     gene            complement(4010..>4876)
                     /locus_tag="VFQ23_01780"
     CDS             complement(4010..>4876)
                     /locus_tag="VFQ23_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905330.1"
                     /translation="VRSNLHTVLFAARDASAAERPFRGADLELFFVLARQAVVAMENA
                     RLYADLRDYVKRVEDSQEALLRAEKMAAAGRLTASIAHEVNNPLQSVQNCLHLAGRED
                     VPPEKRKEYFDLAKNELDRLMKTMQRMLDFYRPGAVRVEQIDVLDLLNHVLNLTSQQL
                     SQRHIEIKADLPESMPLVYAVSGQIQQIFFNLILNSFDAMPAGGDLKIKARALEDGVE
                     VIFQDSGPGIPEERRNNIFEPFFSTKDGGTGLGLTVSYNIVTSHGGTLDLLDKDEPGA
                     CFRLFLPSGDKQ"
BASE COUNT         1196 a         1204 c         1223 g         1253 t
ORIGIN      
        1 tgggaagcca taaacgagaa aaccccgcac gcgatcatcc tggatatttt tatgcctgaa
       61 atggatggct ttacaattct ggagaatctg cgtgagcacc ccacactgag agatgttccg
      121 gtgctagtcg tcagtggcgg cggattaacc aacgagcagc aacagcaact caatgagtac
      181 ggacaacgcc tcattatgaa aggttcgtta agcgaagaag agttgctctc aagtattgag
      241 gccgccctga agaaaatagg agcctaagaa gctctagatg ccgtttttcc aaaaggtagc
      301 tctgaaacgc atttactatg tattcaaaag aagtgcaggt acaatagagt atcgaacagt
      361 gatctcctgt caattaaggg gattgcgcca tgacaataga taaacaacaa aaagagaatc
      421 ttcccatatg tcatttgtga tcagatgttt ggattgtggc tatgccgcgc cattcaatcc
      481 gagttccatg cattgtcctc aatgcaacag tcagtggcgc gaggctgaat acgatttagt
      541 cgaggtggca aagacttttc catccgaatt aaagaagcgt ccattcgatc tgtggcgtta
      601 tagtgaatta ttaccgattc gcaatccgaa ccccacttta agactcggtg aaggcggaac
      661 acctcttatt caagctgcaa atttgggaat gatgttgggt ctgccaaacc tgttcatcaa
      721 ggatgagcgg cagggtccaa cgtcgtcgtt taaagaccga caggcggccg ttactattgc
      781 ggcactgaaa gaggggggtg tgacagaaat ggtggctgcc tccaccggaa atgtagccat
      841 tgcgctttca gcgtatgcgg ctcgagccgg gatcaagctg tgggccttcg tcaccagtct
      901 cgttcccggg gtcaagatgc gtgaaattgc cctgtatggg agtcaggtgg ttaagatcac
      961 cggctcttat gatcaggcaa aacaagttgc ggcggaattt gcgcgtcagc gtggccttta
     1021 tcaggatatg ggtgcacgga cagtgacagc tgtcgaagcg atgaaaacac ttgcctttga
     1081 aatagctgaa caacttactc tggctcaggg cttcacaacc gggtcaactt ctgagacacc
     1141 gaaatggcgc acgcccgatt ggtatattca agcggttagt ggcggcatgg gcccccttgg
     1201 ggtctataag ggctttcgtg aattgaaaca actgggcctg atcaatcgga ttcctgcgct
     1261 cgttccaatt caagcggaag gttgtgcacc tatggtggaa agttggaaga aaggcctgga
     1321 aatagcagag cctgttcttt caccgcagac tcgcattgag acactcgcca ctggcgatcc
     1381 cggtcggacc tataccatgt tgcggaatca ggttaatgag acaggcggag tgttcgaaag
     1441 tgtctcggat gaggactcct ttcgagccat gcacgtcctc gccaaaatgg agggtatttc
     1501 ggcagaacct gccgcaggca ctgcattcgc aggcttgttc aagcttgtgc gggctggcgt
     1561 cattaaacct acagatacag tcgtggtgaa ctgcacgggg cacacactcc ctgcagagca
     1621 gttcctcttc ggagaaaact ggactcgaga tattgatttg ctcccacggg cagaacaatc
     1681 tccaactcca caggaaggct tgctctctgc gctgaacaat gtgaccccaa accggttctc
     1741 gcgcgttgcc gtagttgatg atagtccgga ggcgcgtcgt ctcatccgcc gtatccttca
     1801 atcgcaagga gatttcgaaa tctttgaggc catagatggg cgggaagcga tagaattagt
     1861 caccagggaa cgcccagacc tcgttatcct tgacttgatg atgcctgaag tcgatggctt
     1921 tgctgtattg gatgccttgc gtagccagcc tgatacagcc aacatccctg tgatcgttgc
     1981 aactgcgaaa gaactgactg tggatgaaaa ggcgcgctta cagggacaga tccaatcact
     2041 tatgcagaaa ggcgatttcc tgaatgatga atttcttgaa gaagtgcgct ccctcattcg
     2101 ttgaccccgg gaactgttac ggctctgtat attcaactac aacgacgggc aactacaatc
     2161 acccgtcgtt gtgatttaaa ataagaaata tgagcaggat gcaaaatggt cgtttggcag
     2221 ggataaacgc tgcgcttatc tctgctttct tcttaggact ggcccccgtc tttggcaaag
     2281 ctgccatggg aggcgatcat ttctctcctt ttgcagtggt tgcgctgcgc accagcgccg
     2341 cagctttgtt gttgtttctt attatggcca tcttcaatag aaaattcctc tatatttatg
     2401 gagccggatt acttggatgt gtcatcgctg gaagcatcaa tggaattggg tcgctttttt
     2461 attacgtggg gctaagcaaa ctgaacgcca gcatagcaca aatgctttac tctctgtatc
     2521 ctttttttgt cgccacctgg ctcattcttg acagacagcc gctctccaga cttactacat
     2581 ttcgaatagc gatcgctctt gtctccgcca ttctgcttac acaatctggc gctgggacgg
     2641 ttgatcccat cggagtcgct tgtatgatta ttgcagccgg attctatgct gctcatattc
     2701 caattaatca acgagtcctg tacgacgttc cgccaccgac tgtaacgtta tacactctaa
     2761 tctccatgag cgtgatagtc gtcggtgcat actttatttt tgatcgaagc cagccgactg
     2821 gggaagctcc gtggataccg gtctttgcat taactatggt tacatttttc tcgcgtctca
     2881 cacttttcct gggaattaag catattggtg gaatgcagac agcgctattg ggcctgggag
     2941 aattgatcgt cgccatctta ttcagccatc tcctgctcaa tgaaacgttg agcaatgtcc
     3001 aatgggttgg cgtcgcatgt ttgggaatca gccttcttat ggttttgttc gagaaacctc
     3061 tccctcaccc acctgttcac ccacacgggt tgttgagctg gctgcaaccg cctgacttcc
     3121 cggaggactt ctacaagcat tgagaatcaa aaatgaacga ggttggtgcc tcgttcattt
     3181 tttggggttc tagaagatta cttatcgcct tcgtagagat agccggttcc gcgcacgctc
     3241 acgatgcgat ccgagagcgc agggaaggca tccagcttat ggcgcagccg gctcaccaat
     3301 ggacgcagga tttcgggggc ttcacgctga gacgtatcat atccctgtac aagcaataca
     3361 agctcacgat gtgagtagac acgcccaggg ttttcaataa ggacccgcaa gagacgccct
     3421 tcggcagggg tgagatgaat gatttgatct tttttgcgaa cctgtcggcg tgagagatcg
     3481 atatgtgtac catccttcag cgaaaactcc gccatccctt catcgacatc aatggacgcg
     3541 gttccgccgc gcatttttag gcgcgcttca cgccgggtta agcctttctt tacactattt
     3601 accacctgtg ctggcgggac tggctttaga agataatcat gaatgcgcaa acgcaacgcc
     3661 tgaatggcag attccgtcgt gccaaaggca gtcaacaaaa tcacttcagt ttctggcgaa
     3721 acctgattga ccacctgaac cacttccagc ccatccatgc caggcatgcg aaggtcaacg
     3781 accattaatt caacgggatg cgtccgcacg tactccaccg cagcctgccc attgggtact
     3841 gcgtttacga tatatccctc gagccgcaaa atgtccgaaa gcgactggcg ggcgacgggc
     3901 tcatcgtcta ctacaaggat atttgacttc attaatcatc tcccatgcaa taatttatat
     3961 tcaataaaca caaagattta taatgatccg tggagtactt cgtgtttaat cattgtttgt
     4021 cgccactggg caagaataat cgaaaacagg cacctggctc atctttatcc aataggtcaa
     4081 gggtgccacc gtgcgaggtg acgatattgt aactcacagt cagtccaagg cctgtgccac
     4141 catcctttgt gctgaagaat ggttcaaaga tattgtttcg ccgttcctcg ggaattcctg
     4201 gcccgctatc ctggaaaatc acttctacgc catcttcgag tgcccgcgct ttgatcttta
     4261 agtctccacc tgcaggcatg gcatcgaaag aattcaggat taagttaaag aatatctgtt
     4321 ggatctgacc actcacggca tagactaagg gcatcgattc tggtagatcg gcttttatct
     4381 caatatgccg ctggcttaat tgctgggagg taagatttag aacgtgattt agcaggtcca
     4441 gcacatcgat ttgttctacc ctgacagcac caggacgata aaaatcgagc atacgctgca
     4501 tggttttcat gaggcgatca agttcgtttt tggcgagatc gaaatattcc ttccgttttt
     4561 cgggcggaac atcctcacgc ccggctaaat gcagacagtt ttgtacagac tgtaaagggt
     4621 tgttgacttc atgggcgatg gatgcggtta accgtcctgc agctgccatt ttttcggcac
     4681 gcagtaaagc ttcctgtgaa tcttctaccc gtttgacgta gtcacgcagg tctgcataaa
     4741 ggcgcgcatt ttccatggcc accacggcct ggcgggcaag cacaaagaag agttccagat
     4801 ctgcgccgcg aaagggtctc tctgccgcgc tggcatctcg tgcggcaaac aatacagtgt
     4861 gcaaattcga gcggac
//
LOCUS       DASTJP010000057         5561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_674731, whole genome shotgun sequence.
ACCESSION   DASTJP010000057 DASTJP010000000
VERSION     DASTJP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674731"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="VFQ23_01785"
     CDS             <1..1444
                     /locus_tag="VFQ23_01785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET9905331.1"
                     /translation="FSLWWKDLDAENAERLMSASGDYRYYLEEMRHFKPHTLTELEEK
                     IVNLKDVTGSNALINLYDAITNRYVFKLEVNGEVKELTRPQLQPYIQGSDPDLRAKAY
                     QELYRVFGEDGAILGQMYQTRVRDWYNENISLRKFSSPMSVRNLANDVPDEAVNALLE
                     VTKKNSKIFQRYFKMKAKFVGVDKLRRYDIYAPVAKSDKPFEFDTAAQMVFDAFSAFD
                     PRIGDLARRVFDQDHLDSEVRKGKDGRAFCASINPEDTPYVLMNFTGRARDVATLAHE
                     LGHAIHAMLASHHTAFTFHSSLPLAETASTFGEMMLTEKLLSEETDEAVRRDILFKQM
                     DDAFATILRQIYFALFEREAHDMIQKNASVDELCAAYMNNLKEQFGDSLELSDDFKWE
                     WVSIPHIYHTPFYVYAYAFGQLLVFSLYQQYKAEGESFKPKYLKILSAGGSEAPEKIL
                     SDAGIDIRSTKFWQGGFDVLSRMVDELEKL"
     gene            complement(1510..2064)
                     /locus_tag="VFQ23_01790"
     CDS             complement(1510..2064)
                     /locus_tag="VFQ23_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905332.1"
                     /translation="MIYLIGGAPRAGKSVLGKQAAAKLNIGWMSTDLMMELLRVKNEE
                     GVKTEWNANPEAITANAVWFLPYLERLIWVFSSLAEDYLVEGADFLPAQVAQLSKQYE
                     IRSVFVGCSEITLEQLDQFLGRSPGYAFLSEDMKRQVVHDVPLWSEFIRQEAERFGYP
                     YIDTSGDFPSRLHEAETLLTTNVA"
     gene            complement(2086..2871)
                     /locus_tag="VFQ23_01795"
     CDS             complement(2086..2871)
                     /locus_tag="VFQ23_01795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9905333.1"
                     /translation="MAEVNYESMWWGYIYDQMMKEDLSDQLADRLRFYRSNLRQVAGP
                     ILECGCGTGLIFLPLLADGLDLYGFDISQAMLNTLKRKVEAQRFKDVNRRISVQDLES
                     FHYGQVFDAIIIPSNTFSMLTTQEAQINALKNMHAHLAPKGKLLLDIQLIGMRDLVEG
                     EIETTGRWHTWTHPETGRTIRQRVDGRVDFNRQLVLDRCFIEYEDQREEFPMNARWIF
                     KEEFQLLLRLGGFERWEAFSTPEREPLEISQEGMHSYWIIHKK"
     gene            complement(2933..4753)
                     /locus_tag="VFQ23_01800"
     CDS             complement(2933..4753)
                     /locus_tag="VFQ23_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HET9905334.1"
                     /translation="MTDTLWSQPHHDGSETYVPEPNPKLGGKLNVFLRVPKTSDVTNA
                     WVRVLCDGEPELVQATVDKQDQRDTWLRAELRVVNPVVSYRWLLDGGTYGYQWLNGGG
                     IHSHDVADASDFRISTYDPPPLWATGTMYQIFPDRFAKSVDRPAPAWAKPSDWDDPVI
                     GEGPDTPRQYYGGDLDGITDHLDYIASLGVGTIYLTPFFPAESNHRYNASTFKRVDPL
                     LGGDAAMARLASAAHARGIRLMGDLTTNHCGDTHEWFQAALADPNSPEATFFFFTQHP
                     HHYDAWWGMKEMPIFDHRSLELRRRLYDGPDSVVAKWLGPHALDAWRIDVANMTGIHG
                     DINLSHEVATIIRRTMAQVTSESFLQAESNHDASRDLYGDGYQGTMNYAAFTRPLWQW
                     LLPPQAVPYKHGKYPLLPNLPGKLIAAAMQELSGVTAWQGTLHAMNLIGSHDTHRVIS
                     LLGDPKLVDVAFGMLAAYPGVPMIYAGDEIGLATMGPEYARIPMPWSHPERWDQRRLA
                     NTRALFGERANSTALRRGGLRWLSIEDDSLTFLRESPNESVLVHAARADHTPVQIPAA
                     VLGSELKGLAEATDLRADADGIITLPANGPAFGMWRLSAI"
     gene            complement(4823..5251)
                     /locus_tag="VFQ23_01805"
     CDS             complement(4823..5251)
                     /locus_tag="VFQ23_01805"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9905335.1"
                     /translation="MSTSNSNHPVFELRIALTASDYERSMKFYCDGLGLEPSALWSNG
                     GGRALILDMGKATLELFDEIQAATIDQIEAEQRISGQVRFALQVPDVKAAMERLLEHG
                     ATLIHPPIITPWGDYNVRLQDPDGMQITLFQAPASSDESR"
     gene            complement(5321..>5561)
                     /locus_tag="VFQ23_01810"
     CDS             complement(5321..>5561)
                     /locus_tag="VFQ23_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905336.1"
                     /translation="VKKRLVLLTSFLSTLVGILIFRAGESLWPLWVVENRLRIIAILL
                     LVIICLIVVFPIMIEASTNPRTLSGPGDGDIPFTD"
BASE COUNT         1381 a         1399 c         1418 g         1363 t
ORIGIN      
        1 cttcagcctg tggtggaagg acctggacgc ggaaaatgcc gaacgcctga tgtctgcctc
       61 gggtgattat cgttattatc tcgaagagat gcggcacttc aaaccccata cattgactga
      121 actggaagaa aagattgtca acctcaagga tgtgacgggc tcgaacgcac tgatcaattt
      181 atatgatgcc atcaccaatc gttatgtctt caaactcgag gtgaacggtg aagtgaagga
      241 acttacacgt ccgcagttgc agccttatat tcagggttcg gatcctgatc tgcgcgccaa
      301 ggcctatcag gaactctatc gtgtttttgg tgaagatggt gccattctcg ggcagatgta
      361 ccagacccgc gtacgcgact ggtataacga aaatatttct ttaagaaaat tcagcagccc
      421 aatgtctgtt cgaaatcttg ccaatgatgt tccggacgag gcagtgaatg ccttgctgga
      481 agtgaccaag aaaaactcaa agatcttcca gcgttatttc aagatgaagg ctaagtttgt
      541 cggcgtcgat aagctccgac gttatgatat ttatgctccg gtggccaaat cggacaaacc
      601 gtttgagttt gatactgccg cccaaatggt ttttgatgcc ttctcggctt ttgaccccag
      661 gatcggagat ctggcccgtc gcgtatttga ccaggatcat ctcgatagtg aagtgcgcaa
      721 agggaaagat ggacgtgcgt tctgtgcttc catcaacccc gaagatactc cctatgtgtt
      781 gatgaatttc acaggccgcg cccgtgatgt tgcaacgctg gcccatgaat taggtcacgc
      841 catccatgcc atgctggcgt cacatcacac tgcgtttacc ttccattcgt ccctgccgct
      901 ggcagagacg gcctccacct ttggtgagat gatgctcacc gaaaaactgc tctccgaaga
      961 gaccgatgaa gccgtgcgcc gcgacatcct cttcaaacaa atggatgacg ccttcgcgac
     1021 cattttgcgc cagatctact ttgcgctctt cgaacgtgaa gcgcatgaca tgattcaaaa
     1081 gaatgcctcg gtggatgaac tctgtgcggc ttatatgaac aatttgaaag agcaatttgg
     1141 cgactcactc gaactcagtg acgacttcaa atgggagtgg gtgtcgattc cacacattta
     1201 tcatacgccg ttctacgtat acgcgtatgc gtttggtcag ttattagtgt tctcgctgta
     1261 ccagcaatac aaagccgaag gtgaatcctt caagccgaag tatctcaaga tattatctgc
     1321 gggaggttcc gaagcgccag agaaaatttt atcggatgca ggcattgaca ttcgctccac
     1381 gaaattctgg caaggtggct tcgatgtact gagcaggatg gtggatgagt tggagaaact
     1441 ttaacagtaa gtagtcggtt aaaaaggaaa caggtaaata aggtttgctt cttgtttacc
     1501 tgtttttatt taagcaacat ttgttgtaag tagtgtttca gcttcgtgca gacgtgacgg
     1561 gaaatcaccg ctcgtatcga tatatggata gccaaaacgt tcagcttcct gacgaatgaa
     1621 ctcgctccaa agtgggacat catgaacgac ctgacgcttc atgtcttctg atagaaatgc
     1681 gtatccaggt gaacgcccca gaaattgatc aagctgctca agagtgattt cggaacatcc
     1741 aacgaacaca gagcgtatct cgtactgctt tgaaagctgc gcaacctggg ctggaagaaa
     1801 atcagcgcct tcaactaagt aatcctctgc gagagaactg aatacccaga ttaacctttc
     1861 caggtagggc agaaaccata cggcatttgc tgtgatggcc tcaggatttg cattccattc
     1921 agtttttacg ccttcttcat tctttactct tagaagttcc atcatgaggt cagttgacat
     1981 ccatccgata ttcaatttcg cagcagcctg tttccccagg acgctcttac cagctctggg
     2041 cgctccgccg attaagtaga tcatgtcgat cctccacaaa atccttcatt ttttatgaat
     2101 aatccaatag ctatgcattc cttcctggct gatttctagc ggttcgcgtt caggtgtact
     2161 gaaagcctcc catcgttcga atcctccaag acgcaaaagc agttgaaatt cctccttgaa
     2221 aatccatcga gcgttcatgg gaaactcttc tcgttgatct tcatactcga tgaagcaccg
     2281 atccaagaca agctgacggt tgaaatcaac tcggccatcg acgcgctggc ggatggtgcg
     2341 accagtctca gggtgcgtcc atgtatgcca gcgccctgtt gtttctatct caccttctac
     2401 caagtcgcgc attccgatga gttggatatc aagaagcagt ttccctttgg gcgccaggtg
     2461 ggcatgcata tttttaagcg cattgatctg tgcctcctgg gtggtcaaca tggaaaacgt
     2521 gttggatggg attatgatgg catcgaagac ctgaccataa tgaaacgatt cgagatcttg
     2581 tacggaaatg cgtctattca catctttgaa tctctgtgct tctactttgc gcttgagcgt
     2641 attgagcatc gcttgagaaa tgtcaaagcc gtatagatcg agaccatctg ccagaagcgg
     2701 aaggaatatc aaacctgtac cacacccaca ttcgagaatc ggaccagcaa cctggcgaag
     2761 atttgaacga taaaaacgaa gacgatcggc cagttggtct gataaatcct ctttcatcat
     2821 ctggtcataa atataccccc accacatact ctcatagttg acttctgcca taatggtctc
     2881 ctgtattcat aaccgtacca ctctggagaa tgattggttc ccatatcttg tactagatag
     2941 cagatagtct ccacatccca aatgcaggtc catttgcggg gagggtgatg atgccgtcgg
     3001 cgtcagcgcg aaggtctgta gcttcggcaa gacctttcaa ttcagaacca agaactgctg
     3061 ccgggatttg gacaggagta tggtcggcgc gcgcggcgtg gacaagaaca ctctcattgg
     3121 gagattcgcg caggaaggtc aatgagtcat cctcgatgct cagccagcgc aagccaccac
     3181 gtcgaagagc ggtgctgttt gcacgttcgc cgaagagggc gcgggtattt gcaagtcgtc
     3241 tctgatccca gcgctccggg tgcgaccagg gcatagggat gcgagcatat tcaggaccca
     3301 tcgtagcaag cccgatttca tcaccggcat aaatcatcgg gacgcccgga taagctgcga
     3361 gcataccaaa ggcaacatcg accagtttag gatcgccaag caaagaaatg acgcgatgtg
     3421 tgtcatgtga gccgatcagg ttcatggcat gtaatgtgcc ttgccacgca gtcaccccag
     3481 ataactcttg catggcggct gcaatcaact ttcccggcag gttgggcagt aggggatatt
     3541 tgccatgctt gtatggcact gcttgtggtg gcagcaacca ttgccaaagc ggacgtgtga
     3601 acgccgcgta attcatcgtt ccctgataac cgtcgccgta gagatcgcgg ctggcgtcat
     3661 ggttcgattc agcctgcaga aacgactcgg aagtgacctg cgccatggtg cggcgtatga
     3721 tggttgccac ctcatggctg aggttgatgt cgccatgaat gccggtcatg ttggcgacgt
     3781 caattcgcca ggcatccagc gcgtgcggac ccagccactt agcgacgacg gagtccggac
     3841 cgtcatagag gcgccgtcgc agttcgaggc tgcgatgatc gaagatcggc atttccttca
     3901 tgccccacca cgcgtcataa tggtgcggat gttgcgtgaa aaagaagaac gtcgcttcgg
     3961 gagagttggg gtcagccaac gcggcctgga accactcatg tgtatctcca cagtgattgg
     4021 tggtaaggtc gcccatcagg cggatgccgc gggcatgcgc ggcggaggcg agtcgcgcca
     4081 tagctgcatc gccgcccaac agcggatcca cacgcttgaa cgttgaggcg ttgtagcgat
     4141 gatttgattc cgctgggaaa aacggcgtca gataaatggt cccaacccca agggatgcga
     4201 tgtaatccag gtgatcggta atgccatcca ggtccccacc ataatactga cgaggcgtat
     4261 ctggtccttc gccgatgacc gggtcatccc aatctgaagg ctttgcccat gcaggagcag
     4321 gtcgatcaac ggacttggcg aaccggtccg gaaagatctg gtacatggtg ccggttgccc
     4381 acaagggtgg agggtcatag gtgctgatgc ggaagtcaga agcgtccgcg acatcatgac
     4441 tgtgtatccc tccaccgttc agccactgat atccatacgt tccgccatcc agcagccagc
     4501 ggtaacttac cacgggattg accacgcgca gttcggcgcg cagccaggtg tcacgctgat
     4561 cctgcttgtc gacggttgcc tgcaccaact cgggttcacc atcacacagg acgcgtaccc
     4621 aggcattggt gacatcactc gtctttggca ctcgcagaaa gacattaagc ttgccgccaa
     4681 gtttcggatt cggctcagga acatacgttt ccgaaccatc gtgatgcggc tgagaccaaa
     4741 gggtatcggt catggttttc taactcccag gttttgaaat gatgatgtca atcacttgga
     4801 ggatggcatg aaatcaagaa tactacctgg attcatcact gcttgcgggt gcctgaaaga
     4861 gtgtgatctg catgccatcc gggtcctgaa gccgcacgtt atagtcgccc cacggagtaa
     4921 tgatgggcgg atgaatcagg gtagcgccat gctcgagtag gcgctccatg gcagccttca
     4981 catccggcac ttgcagtgca aagcggacct gcccgctgat gcgctgttcg gcttcgatct
     5041 gatcaatagt ggcggcctgt atttcgtcaa agagttcgag ggtagctttt cccatatcca
     5101 ggatgagcgc gcggcctccg ccattgctcc acagcgcgct gggttcgagt ccgagtccat
     5161 cgcaataaaa tttcatagat cgttcataat cgctggctgt gagtgcaatc cgcaattcaa
     5221 aaacaggatg attcgaattg gaagttgaca tattgggaag agatcctttc aagtcaagtt
     5281 actatctgaa tttagatatc cacctttcat gatgctgtca tcaatctgtg aaagggatat
     5341 ctccatcgcc tggtccagaa agagtacgcg ggttggtact agcctcaatc ataattggaa
     5401 atactacaat taagcagatg ataacgagca ataagattgc tatgattcgc aaccgatttt
     5461 ccactaccca taatggccat aaagactcgc ctgccctaaa gatcaggatg cccacaagcg
     5521 tgctcaagaa tgaggtcaat aaaactagtc ttttcttgac g
//
LOCUS       DASTJP010000058         4387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1383506, whole genome shotgun sequence.
ACCESSION   DASTJP010000058 DASTJP010000000
VERSION     DASTJP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383506"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFQ23_01815"
     CDS             <1..522
                     /locus_tag="VFQ23_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9905337.1"
                     /translation="QYPNQYVSGMRPSLPRGWFNWAFWQYSDKGRINGINARVDLNVF
                     NGTLEELYNLASATPVSPDATPKKHTVAAGDTFESVAVQYGVTVRELVSANPQLLKTG
                     DQLSIPASDAIPNENGGSSPSAPTQRTHTVRAGENLTTIAIKYGTTVAAIASANSITN
                     INNIRVGQVLIIP"
     gene            656..2866
                     /locus_tag="VFQ23_01820"
     CDS             656..2866
                     /locus_tag="VFQ23_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET9905338.1"
                     /translation="MSLPPVPSFKPIANLKSIVVAPNVRFTVLTDRIIRIEFSQVSHF
                     EDRPSQVFWYRDQPVPKFKKEITDKVIEIETNYLHLIYKIGRTGFTPRNLSIKIKNGK
                     TIWRFGASAAKAGNLKGTARTLDGVAGKTRLEDGLVSTAGWSVVDDSKTLVFDENGWL
                     VPRTNRKSLDLYFFGYGDDIPGMLRDYTRIAGEIPMIPRYILGNWWSRYWAYTQEELQ
                     SLMQEFRAREIPLSVCIIDMDWHLTKTGNQSPGWTGYTWNRELFPDPQGFIAWLHSQG
                     LRTALNLHPADGVHSHEEQYAEMAKWMGIDPNSKTPVAFDISDPRFMEGYFELLHHPF
                     ETPASVRAGQRAKRVGEARNGVDFWWMDWQQGKSSRVKGLDPLWGLNHLHFQDIGRNG
                     RNRPFVFSRWGGLGNHRYPIGFSGDTIIEWSSLAFQPYFTSTAANVAYGWWSHDIGGH
                     FRVDGTPELYLRWVQFGVFSPIFRLHSSNMPELDRRPWGNKPERIFQAAREAMQLRHA
                     LIPYVYSMAWRAHQTGLSLVTPMYYGNMDSSEAHKAKDQYFFGSELIVAPVVKPVNEK
                     TGFTTQKIWFPRGTWFNFFTGEQIKGGSWQTVKSTLEDIPVYAKAGAIIPLAPKVGWG
                     GIENPNELDIYVFPGKDNAFDLYEDDGETTDYQRGKYAITTFTLKKNTFTIQPVTGDT
                     SLTPSQRIYRIHLRGVEEKTRVSLPGQYDASTRTLILDVVTLQPTESLQVKLKR"
     gene            complement(2939..4282)
                     /gene="asnS"
                     /locus_tag="VFQ23_01825"
     CDS             complement(2939..4282)
                     /gene="asnS"
                     /locus_tag="VFQ23_01825"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET9905339.1"
                     /translation="MAEQVSVSQISKYIGQEVTIKGWVYNRTDKGKLCFLLVRDGSGF
                     VQCVAFKGDLPEDLFNKIMRLPQESSVIVTGAVREDKRAPGIPGGFEVGIKDLQIVQE
                     AAPDYPMSLKDHGPDFALDHRHLWIRMQSQWATLKVRVNVMSAVREYLDGRGFFNLDT
                     PILTPAAAEGTTTLFETPYFDEGFAYLAQTGQLYNEANIFAFGKVYCFGPTFRAEKSK
                     TRRHLTEFWMLEPEIAFCDLDGLMELEEGLITHIVQTVLRDCAAELKFLGRDISKLEN
                     VTGTFPRMSYDDAAKYLIDLYEKETDPERKELLKFEWGMDFGAPHETELTKLHAKPLF
                     VYGYPSAVKAFYMEPWPDRPEVCKSVDLLAPEGYGEICGGSERMSDHDALLARIHKEG
                     LPEEAFKWYLELRKYGSVPHSGFGMGVERVTAWLCGIEHIREAIPFPRTIKRVYP"
BASE COUNT         1162 a         1104 c         1055 g         1066 t
ORIGIN      
        1 caatatccta atcaatatgt ctcggggatg cgcccctcgc ttccaagagg ttggtttaat
       61 tgggccttct ggcaatatag tgacaagggt cgtatcaatg gcatcaatgc ccgggtagac
      121 ttgaatgtgt tcaacggtac cctggaggag ctatataacc tggcgagcgc aactccagtg
      181 agtcctgacg caacgccgaa aaaacatacc gtcgcggctg gcgatacgtt cgaatcggtc
      241 gcagttcaat acggcgtgac agtccgagaa cttgtcagcg caaaccctca attgctgaag
      301 acaggtgatc agttgagtat tccggcttct gatgcgattc cgaacgaaaa tggaggcagt
      361 tcgccatccg cgccgacaca gaggacgcat accgtcagag ccggtgaaaa cctcaccacc
      421 attgcgatca agtacggtac taccgttgcg gcgatcgcct ccgccaatag tattacgaat
      481 attaacaaca tcagggtagg ccaagtactt atcatacctt gagaaaatag acaggaaaat
      541 ccccgttaca agacggggat ttttagtgat cccttcatca ctactttgca attttcggta
      601 aacttagtta aactcacatc agaaaaaaaa cacttcaacc taaccgaggg agtcgatgtc
      661 tctaccacct gtcccgtcgt tcaaaccaat tgctaaccta aaatccatcg tggtggcacc
      721 caatgtccgt tttacagttc taaccgatcg gatcatccga atcgaattta gtcaagttag
      781 tcactttgag gatcgtcctt ctcaggtttt ttggtatcgt gaccaacctg tgccaaagtt
      841 caagaaagaa atcaccgata aagtcattga aattgaaacg aactatcttc atctgatcta
      901 taagatcgga cgcacgggat ttacccctcg caacttaagc atcaagatca aaaatggaaa
      961 aactatctgg cgctttggag cttccgccgc caaagctggc aacctgaaag ggacagcccg
     1021 cacgctggac ggcgtcgctg gaaaaacgag acttgaggat ggtttggttt caaccgcagg
     1081 ctggtcagtc gtagacgact cgaaaactct cgtgtttgac gaaaatggct ggcttgtacc
     1141 acggacgaat agaaaatcgc tggaccttta tttctttggc tacggcgacg acatcccagg
     1201 gatgctgcgc gattacacaa gaatcgcggg tgagattcca atgatcccgc gttacattct
     1261 gggcaactgg tggagtcggt actgggccta cacccaggaa gaactgcaat ccctcatgca
     1321 ggaatttcga gcgcgcgaga ttccgctttc ggtttgtatc attgacatgg attggcacct
     1381 cacaaaaacg ggcaatcaat cgcccggctg gacaggatac acctggaacc gcgaactttt
     1441 ccctgacccg cagggattca ttgcctggct tcactcgcag ggattgcgaa ctgctctcaa
     1501 cctgcatcct gccgatggag tgcattcgca cgaagaacaa tatgccgaaa tggcaaaatg
     1561 gatgggtatt gacccgaatt caaagacacc tgtggctttc gatatttccg acccgcgctt
     1621 catggagggt tactttgaat tactgcatca tccgtttgaa actcccgctt ccgttcgcgc
     1681 tgggcaaagg gcgaagcgcg taggcgaagc gcgtaacggc gtcgatttct ggtggatgga
     1741 ttggcaacaa ggtaaatcgt cgcgggttaa aggcctggat ccgttatggg gattgaatca
     1801 tttgcatttt caggatatag gcagaaatgg acgaaatcga ccgtttgtct tttcacgctg
     1861 gggcgggctt ggcaatcatc gctatccgat cggttttagc ggtgatacga tcattgaatg
     1921 gagttcgctg gcctttcagc catatttcac ctccaccgcg gcgaacgtcg cttatgggtg
     1981 gtggagtcat gatatcggcg ggcattttcg cgtcgacggc acacctgaac tctatttgcg
     2041 ttgggttcaa tttggggtct tcagtccgat cttccgtttg cacagttcga acatgccgga
     2101 gcttgaccgc cgtccatggg gcaacaaacc cgagcgtatt ttccaggccg cgcgcgaggc
     2161 gatgcaatta cgtcatgcgt taattcccta cgtctatagt atggcgtggc gcgcccatca
     2221 aacaggattg agtctggtta cgccaatgta ttatggaaat atggattcgt cggaagcaca
     2281 caaggcgaag gatcaatact tctttggctc agaattaatc gtcgcccctg tggtcaaacc
     2341 agttaacgag aaaacgggat ttactacaca aaaaatctgg tttccgcgcg ggacctggtt
     2401 taattttttc acaggcgagc aaatcaaagg tggaagctgg caaacggtca aatccacact
     2461 cgaagacatt ccggtgtatg ccaaagcggg agcgattatc cctcttgcgc caaaagttgg
     2521 atggggaggg atcgaaaatc caaacgaact cgatatttac gtttttcccg gcaaagataa
     2581 tgcgtttgat ctgtatgagg atgacggtga gaccacagat tatcaacgcg gcaaatatgc
     2641 gatcacaaca ttcactctta agaagaatac cttcaccatt caacctgtca ctggagatac
     2701 aagcctgaca ccctcacaac gcatctatcg tatccatctg cgcggtgtgg aggaaaaaac
     2761 aagggtctct ttgccaggcc aatacgacgc atcgacacgg acactcatat tggatgtggt
     2821 cactctacaa ccaactgagt cgctccaggt gaaactgaaa cggtaataag ctccaatagc
     2881 attaaacaaa gaaggacata ctatgccagt atgtccttct taaattctga agactcctct
     2941 aaggatacac ccgcttaatt gtccttggga acggtatcgc ttcacgaata tgttcgatac
     3001 cacacaacca cgccgtgact ctctctacgc ccatgccgaa gccagaatgt ggaacggatc
     3061 catactttcg caattccaaa taccacttga aagcttcttc tggtaagcct tccttgtgaa
     3121 tgcgagccag caaagcgtcg tggtcagaca tgcgttctga gccgccacag atctcgccat
     3181 agccctcagg ggcaagcagg tcaaccgatt tacacacttc cggccgatct ggccagggct
     3241 ccatataaaa agccttaacc gcgctgggat agccgtatac aaacaaaggc ttcgcatgta
     3301 atttcgtcag ttcggtttca tggggagcgc cgaagtccat gccccattcg aatttgagca
     3361 attctttgcg ttcggggtct gtctcttttt cataaagatc aatcagatat ttggcggcat
     3421 catcatacga catgcgcggg aatgtaccag ttacgttttc cagtttggag atatcacgcc
     3481 cgagaaactt caattccgcc gcgcaatctc gtaaaacagt ttgcacaata tgcgtgatca
     3541 aaccttcttc caattccatc aagccatcca gatcacaaaa agcgatttca ggttccagca
     3601 tccagaactc agtgaggtga cggcgcgtct ttgatttttc agcgcgaaag gtaggaccaa
     3661 aacaataaac ttttccaaaa gcaaagatgt tggcttcgtt gtagagctga ccggtctgtg
     3721 cgagatacgc gaaaccttcg tcgaagtagg gcgtttcaaa aagagtcgtc gtcccttcag
     3781 cagcggcagg ggtgagtatg ggagtgtcga gattgaagaa accccgaccg tccaaatatt
     3841 cgcgcaccgc cgacatcaca ttcacgcgca ccttcaacgt tgcccattga ctctgcatcc
     3901 gaatccataa gtgacggtga tccaacgcaa agtcgggtcc atgatccttg agtgacatgg
     3961 gatagtctgg cgcggcttcc tgcacgatct gcaaatcctt gatgcccacc tcaaatccac
     4021 ctgggatgcc aggggcgcgc ttgtcttccc gtacggcgcc ggtgacaatt acggaggact
     4081 cttgcggcaa tcgcatgatc ttgttgaata gatcttcggg caaatcccct ttgaaggcga
     4141 cacattgtac aaagcctgag ccatcgcgca ccaacaaaaa gcaaagtttg cccttatcgg
     4201 tgcggttgta aacccagccc ttgatcgtta cttcctgacc tatgtacttt gaaatctgag
     4261 aaacgctgac ttgttcagcc atttaaaagt ccttttggtg attgaatgta ccaattatag
     4321 cagagggata tttttctact tgctaggcct aatggtgaat gagtacaaag acaagacatc
     4381 tgtgatt
//
LOCUS       DASTJP010000059         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1254275, whole genome shotgun sequence.
ACCESSION   DASTJP010000059 DASTJP010000000
VERSION     DASTJP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254275"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFQ23_01830"
     CDS             complement(<1..387)
                     /locus_tag="VFQ23_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905340.1"
                     /translation="MSSKLIVIDGKTYNSVDEMPEDVRRNYESAMRQLGDSDRDGLPD
                     LLENLTNPTDQNKNGMPDAIENMVSNVFSSTTRVVVNGAEYNSLDDLPPEVRAQYEQA
                     MGSFDANKNGVPDFLEGFINTTNQTST"
     gene            512..1231
                     /locus_tag="VFQ23_01835"
     CDS             512..1231
                     /locus_tag="VFQ23_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET9905341.1"
                     /translation="MTELLYQTDSYLQEFEADIVSVLNEDRAIVLDRTAFYPGGGGQP
                     CDFGTLQVDGLVYPVEKVKKQGDDVLHFLGGEEALPSIGSTSRGTLDWARRYKLMRTH
                     TALHVLCGTIFRDYGAQVTGGNMEPLKGHLDFEFATMRGELVREIDAAINKEAQAGRD
                     IRVNILPRDEAFQIPDLIRTKINLLPEGISHVRTIEIVGLDLQADGGTHVRNTSEVGK
                     IRVADYKSKGAINKRIYIELE"
     gene            1252..1662
                     /locus_tag="VFQ23_01840"
     CDS             1252..1662
                     /locus_tag="VFQ23_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905342.1"
                     /translation="MKHKKLYPILFAVLALVTASLACLATPTPGASNFYMSTDEAGSN
                     RTTVFSSTDDFFVHFDVAGIEVGTPFQSRWYALDIEGEDPNTPFQTIDYSYEEGIGNI
                     YFQLTNSDGWPAGNYKVEVYMNGTEIGEQLFSVQ"
     gene            complement(1781..>2932)
                     /locus_tag="VFQ23_01845"
     CDS             complement(1781..>2932)
                     /locus_tag="VFQ23_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9905343.1"
                     /translation="IGQPARVAILRFASGPQIVLGDTSGSLLALSMDGAPLWESKLGS
                     SEVRGMDDARIGGQIHLAAATNDGSLGVYDSLGVQVWSSSQEQLRRMRAFDLNGDGNS
                     EVITGGEYGAFFIYSAADGSTVHSASLGQAVSEVREVELNGDPASREIVVGGKDGGVW
                     AFSNTGSELWSASLSDKVNEISGIDINGDGAEEAVIGAESGEVAIFSGNGTRNNLTTH
                     NTPITRIDVGRLGTERYVIIADGNEIQVHQANFNSIPGFQYTPLIVGVIISAVILVIA
                     AILASIPRKPEMKVSFQDKSRESLEAERRMLKESIADVERLRKSGEVTGDAYLARLKR
                     LRGDLAENETAFKAAGFPIKVETIQCPNCGGTLELGMDKCEYCGQVLLS"
BASE COUNT          721 a          765 c          697 g          749 t
ORIGIN      
        1 tgtgctggtc tggtttgtcg tattgataaa cccttccaga aaatctggaa ccccattctt
       61 gtttgcatca aaggatccca tggcctgttc gtactgcgcg cggacctcag gcgggagatc
      121 gtccaaacta ttgtattcgg ctccgttgac aacgaccctt gtggtggacg aaaatacatt
      181 ggataccatg ttttcaattg catcgggcat tccgttttta ttctgatccg ttgggtttgt
      241 caaattttca agcaggtcgg gaaggccatc cctgtcgctg tcgccaagct ggcgcatcgc
      301 gctttcataa tttctacgaa catcctcggg catctcatct acgctgttat aggtcttgcc
      361 gtcaatgaca attagctttg aactcatgac tgcctccagt atgatttcta gtttacagga
      421 agcaacaacg gaatgcaagt cgctttgtaa cctattgatg gatcaacaaa taaccaaaac
      481 tctgtagtac actttattta ttggagatgt tatgaccgag ctgctttacc agactgatag
      541 ttatttgcag gagtttgaag cagatattgt ttctgtattg aacgaggatc gcgcgattgt
      601 tctcgatcga accgcgtttt atccgggtgg tggaggacag ccctgcgact tcgggacgtt
      661 acaggttgat ggccttgttt atccggtgga aaaggtgaag aaacagggcg atgatgtttt
      721 acactttctt ggcggcgagg aagcattgcc gtccatcggc agcacatcgc gcgggacttt
      781 ggactgggcg cggcgctata aattgatgcg tactcatacc gcgctgcacg tcctttgtgg
      841 aacgatcttt cgggattatg gagcgcaggt gacaggaggc aacatggagc cgctcaaggg
      901 tcaccttgat tttgagttcg caacgatgcg aggtgaactt gtgcgtgaga tcgatgccgc
      961 catcaataag gaagcacagg cagggcgcga cattcgtgta aacatcttac cgcgcgacga
     1021 agccttccaa atcccggact tgatccgtac gaagatcaac ctcctacctg agggcattag
     1081 ccacgtccgt acgattgaaa tcgtcggtct ggatcttcag gcagacggtg gtacccacgt
     1141 tcgaaacaca agtgaagttg gaaaaatccg cgtcgcagat tacaaaagca agggcgcgat
     1201 caataagcgg atctatattg agctcgaata atctacttaa ggagagttag tatgaaacac
     1261 aaaaagcttt acccaatctt gtttgcagtc cttgccctcg tgactgccag cctggcctgt
     1321 ctggcgactc ccacgccagg cgcatcgaac ttttacatgt ccacagatga agctggatcc
     1381 aaccgtacga cagtcttttc atccacagat gacttctttg ttcatttcga tgtagccggt
     1441 atcgaggtgg gtacaccttt tcaatctcgc tggtacgcat tggatatcga aggggaggat
     1501 cctaatactc catttcaaac catcgattat tcttatgaag aaggcattgg gaacatttat
     1561 ttccaactga ccaacagcga tggctggccc gccggcaatt acaaggtgga agtttatatg
     1621 aacggcacag aaataggtga gcagttgttt agtgttcagt aaatttaagt gagactgtag
     1681 ttaaacgcag agacgctaag gctcaaagaa cttaaaggcc ttggcgtctt tgtgtcttta
     1741 acgttaaaag tctctaaaaa catccctcaa tgtgcgccat ctaagataac aacacctgcc
     1801 cgcagtactc acatttatcc atccctaact caagtgtacc gccacaattc ggacattgga
     1861 tggtttccac tttgattgga aatcccgccg ctttgaacgc agtctcgttt tccgccaggt
     1921 caccgcgcag gcgtttgagg cgcgccagat acgcgtcgcc ggtcacttcg ccagacttgc
     1981 gcagacgttc tacatcagca atggattctt tcaacatgcg tcgttctgcc tcaagcgatt
     2041 ctcggctttt atcctggaag gacaccttca tctccggctt cctgggtatg gaggcgagaa
     2101 tggcggcaat caccagtatc actgccgaaa tgatcacgcc gactataaga ggcgtatatt
     2161 gaaacccagg gatggaattg aaattggcct ggtgaacctg aatctcattt ccatcggcaa
     2221 taatgacata ccgctccgta cccaatcttc caacgtcgat acgcgtgatc ggtgtgttat
     2281 gcgttgtcag gttattccga gtgccgttcc ctgaaaatat ggccacttct ccactctcgg
     2341 caccaatcac agcttcttcg gctccatcac cattgatgtc aatgcccgaa atttcattta
     2401 ctttatctga taacgatgca ctccaaagtt cactacctgt attggaaaag gcccacacac
     2461 caccatcttt cccgccaaca acaatttcac gagatgctgg atcaccattc aactccactt
     2521 cgcgcacttc gctgactgcc tgccccagtg atgcactatg aaccgtgctc ccatcggcgg
     2581 cgctgtaaat aaagaaggcg ccatactccc cgcctgtgat cacttcactg ttgccatccc
     2641 cgttcaagtc gaaggcgcgc atgcggcgga gctgttcctg gctggaagac cagacctgca
     2701 cacccagtga atcgtagact ccaaggctgc catcattcgt agccgcggct aagtggatct
     2761 gcccaccgat tctggcatca tccatgccgc gcacttcact gctgccgagt ttcgattccc
     2821 acaaaggagc accgtccata ctcaacgcaa gcagactgcc gctcgtatca cccaacacga
     2881 tttgaggtcc agaggcgaaa cggaggatcg caactcgggc aggctgaccg at
//
LOCUS       DASTJP010000060         3499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_41485, whole genome shotgun sequence.
ACCESSION   DASTJP010000060 DASTJP010000000
VERSION     DASTJP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41485"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFQ23_01850"
     CDS             complement(<1..197)
                     /locus_tag="VFQ23_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905344.1"
                     /translation="MADVVIVDAIRTPIGALGGSLASVRPDDLAALVIKEVIQRNKLD
                     PMLVEEVVFGCANQAGEDNRNV"
     gene            369..1265
                     /locus_tag="VFQ23_01855"
     CDS             369..1265
                     /locus_tag="VFQ23_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9905345.1"
                     /translation="MREDQDASRRIYVGAFVRIGQFRRGPKHSNFGGPHQIRGTLLVF
                     PRTSVTITHAGKDPVVADPNTVMFYNHGQIYSRGRLSAKGDLCEWFNFDPGLVADAIR
                     PYNAHVDDHPIEPFSFSHGPSDTTSYLLQRLVVDHIQGEQQPDPLFIEETALQILNRV
                     IENSYRQRGVYPFQAKVSAEREVVDSIRKVLAVRFEQNPSLEEISVELNYSPFHLCRI
                     FRKHTGQSIRQYLNQLRLRASLEYVTQASIDLTSLALQMGFATHSHFTEAFRKTFGTP
                     PSMLRSKSGEYMRQLLSKISIA"
     gene            1318..2496
                     /locus_tag="VFQ23_01860"
     CDS             1318..2496
                     /locus_tag="VFQ23_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905346.1"
                     /translation="MQSAIRLLILSALFLSGCNAAATPTPAPIPSSTQTSEPVALPTS
                     TPAILSADPTPTHLPNAIPTDQLDKSIFQFYEGNPVIQLSNNPGWDNRYIDPGAMIYH
                     DGKFHMFFNGINGFPAPVGVGYATSPDGYQWTRQVSEPVLSATALSDSNLLGSNLFVT
                     SALVEPDGTWILYFYTLAGRTFNDPGEIGRATAPAAAGPWTIDRDPILSPGPDGSWDE
                     VQVTAPDVLKTDTGYLMYYDARGERNVSMIGLATSSDGVHWQKYNDSVTSDPVFAESD
                     PVLTVSNDGWDSKRVIDPNVIQTSDGFEMIYLATSGSGKFTAGEFSFGAATSPDGIEW
                     TKSNLNPVLSNQDHSQWSQAFLASLLHVEETYFLYFDFVTPATSGTNIYLATYTGSLR
                     "
     gene            2670..2915
                     /locus_tag="VFQ23_01865"
     CDS             2670..2915
                     /locus_tag="VFQ23_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9905347.1"
                     /translation="MPAKGWIEVSDTYCKGCELCLSACPQEVMELDMTRLTPKGYHPA
                     HTFKDGCTGCAICALVCPDAAITVYREITKAAAPVAA"
     gene            3023..>3499
                     /locus_tag="VFQ23_01870"
     CDS             3023..>3499
                     /locus_tag="VFQ23_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HET9905348.1"
                     /translation="MTRELWKGNEAIAEASVRAGLEAYFGYPITPQTEILEYLSKRMP
                     ELGRAFVQAESELGAINMVYGAACAGVRVMSTSSSPGVSLMMEGMSYIAGTEVPAVLV
                     NVMRGGPGLGNIAPAQADYNQAVHGGGHGDYHNIVLAPASVQEAIDLTALSFDLAEK"
BASE COUNT          862 a          929 c          885 g          823 t
ORIGIN      
        1 acgttacgat tatcttcacc cgcctggttt gcacatccaa agacgacttc ttccaccagc
       61 attggatcaa gcttgttgcg ttggatcact tcctttataa cgagagcagc cagatcgtca
      121 ggtcgaaccg aggcgagtga ccctcctaac gcaccgatcg gggtgcggat tgcatccacg
      181 atgacaacat cggccatttc atcctccgta ggaacacagc ggcagaattg aacgacaaaa
      241 attttgatgg ttctgctatg cccttacact tatatgaatc gattctacca cctgcagtta
      301 gaacctaatt tgagggaaac ttaaggtaag gcaggttttg atcagacagt ctaatcaggt
      361 aattacatat gcgtgaagat caagatgcaa gccgacgaat atatgtggga gccttcgtcc
      421 gtatcgggca gtttcgacgc ggaccgaaac attccaattt tgggggtcca catcagatcc
      481 gtgggacgct cttggtattt ccgcgtacca gcgtgaccat tacacacgct ggcaaagacc
      541 cggtggtggc agatccgaac acagtcatgt tctacaatca tgggcaaatc tattcgcgtg
      601 gcagactctc cgcaaaggga gacctgtgtg aatggttcaa ttttgatccc gggctcgtag
      661 cggatgccat ccgtccctat aatgcccatg tcgatgacca tcccattgaa cccttttcgt
      721 tctcccatgg ccccagcgac acgaccagct atctattgca aaggctggtg gtcgatcaca
      781 ttcagggaga gcagcagcca gaccctcttt tcatcgaaga gactgccttg caaatactta
      841 accgggtcat tgagaacagt tacaggcaac gcggggtata cccttttcag gcaaaggtct
      901 ccgcagagcg tgaggttgtg gattccattc gaaaagtatt agccgtccgg ttcgagcaaa
      961 atccctctct ggaagagatc tcagtcgagt taaactattc tccctttcat ttatgtcgca
     1021 tcttccgcaa gcataccggg cagagtatcc gccagtactt aaatcagctc cgcctgcggg
     1081 cttccctgga atatgtaacg caagccagca tcgacttgac cagtctggct ctacaaatgg
     1141 ggttcgccac tcatagccac ttcacggagg cgtttcgcaa aacctttggc acaccaccat
     1201 ccatgctgcg cagcaaatct ggggaatata tgcgtcagct gttgagcaag atttcgatag
     1261 cataaattta tccatcgctg tatcgttagc tccaactttt aaaacaagga gcaaacaatg
     1321 caatctgcta tccgtctgtt gattctttct gcgttgtttt tatctggatg caacgctgcc
     1381 gcgacgccca ctcctgcccc gatcccaagc tcaacgcaaa cgtccgagcc ggtcgcctta
     1441 ccaacgtcta ctccggccat tctcagcgcc gacccaacac cgactcacct gccgaacgcg
     1501 attcccaccg accagctgga taagtctatt tttcagtttt atgagggaaa tcctgtcata
     1561 cagctcagca acaaccctgg ctgggataac cggtatatag acccgggcgc gatgatctat
     1621 cacgatggca aattccatat gttctttaac ggcatcaacg gttttccggc tccagtgggc
     1681 gtgggctatg ccacgtcacc cgatgggtac cagtggactc gtcaggtaag tgagccagtg
     1741 ttaagcgcaa cggcattaag tgacagcaat ctacttggtt ccaatttatt cgtcaccagc
     1801 gcactggtcg agccagatgg aacctggata ttgtattttt ataccctggc cggaagaacg
     1861 ttcaacgatc cgggtgagat cggacgtgcc actgccccag cagcagccgg cccctggaca
     1921 attgatcgtg accccatcct gagtcccggt ccagacggct cctgggatga agtccaggtc
     1981 actgcacccg atgtgcttaa aactgacacg gggtatctca tgtactacga tgcccgtgga
     2041 gagcgaaatg tatccatgat cgggttggcg acttcgtctg atggagttca ctggcagaaa
     2101 tacaatgatt cggttacgag tgatcctgtc tttgccgaaa gcgatcctgt tttgacagtg
     2161 agcaatgacg gctgggattc gaaacgagtc attgacccga acgtgattca gacatctgat
     2221 gggtttgaga tgatctatct ggcgacctcg ggctcaggaa aatttacggc gggtgaattc
     2281 tcatttggcg cggccaccag cccggatggc atagaatgga cgaaaagtaa tctaaatccg
     2341 gttctctcca accaagatca ttcgcaatgg tcgcaggcat ttcttgcaag cttactccac
     2401 gtggaagaaa cctatttcct gtacttcgat tttgtcaccc ccgcaacaag tggcacaaat
     2461 atttatctgg ctacttacac tggctcgttg agataaatgc catgatcaaa tcagctgggc
     2521 gagcatgccc gaaataccga tcaaggatag gatgtttgta tgctgtgata aatgacgcat
     2581 ggcactacac tcacaatgtg ggaaaagtga taatcaggct ggaagcatat tgacctattc
     2641 ttgtcaaact tctcagataa ggagtgccga tgcctgcaaa aggttggatc gaagtcagtg
     2701 atacatactg taaaggctgc gagttgtgcc tgagtgcatg tccccaggag gtgatggaac
     2761 tggacatgac caggcttacc cccaaaggct accaccccgc gcacacgttc aaggacggat
     2821 gcacggggtg tgctatttgt gcgctggtct gtccggacgc ggcgatcaca gtctatcgcg
     2881 agatcacaaa ggcagccgcg ccggtcgcgg catgatttga accacaaaga acgcgaagag
     2941 cgcgaagaat ctttcttctt tcctctttca gagtctcagc gacctttgcg tgattcgcgg
     3001 ttataaaaac acggagaaat aaatgacacg tgaattatgg aaaggcaacg aagccatcgc
     3061 cgaagcctcc gttcgtgcag ggctcgaagc ctattttggt tacccgatca ccccacagac
     3121 ggaaattctg gagtacctct cgaaacgcat gcccgaactc ggacgcgcct ttgtgcaggc
     3181 cgaatcggaa ctcggcgcga tcaacatggt ctatggtgca gcttgcgcgg gcgtgcgcgt
     3241 gatgtccacg tcatcgagcc ccggtgtgag cctgatgatg gaaggcatgt catacattgc
     3301 cggaacagaa gtgccggcgg tgctcgtcaa cgtgatgcgc ggtggaccgg gtctcggtaa
     3361 catcgcgccg gcacaggccg attacaacca ggcggttcac ggcggaggtc acggcgatta
     3421 tcacaacatc gtgctggcac ctgcctcggt ccaggaggcg atcgatctga ccgcgctttc
     3481 gtttgacctg gccgaaaaa
//
LOCUS       DASTJP010000061         4086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_568370, whole genome shotgun sequence.
ACCESSION   DASTJP010000061 DASTJP010000000
VERSION     DASTJP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568370"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFQ23_01875"
     CDS             <1..283
                     /locus_tag="VFQ23_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET9905349.1"
                     /translation="VMGGEQAANVLLTIKQDQLQREGKPAMTKEEADEFKRPILEKYE
                     DEGNPYHSTARLWDDGVIDPVDTRQVLGLALSVVLNSPVEENNFGVFRM"
     gene            428..1207
                     /locus_tag="VFQ23_01880"
     CDS             428..1207
                     /locus_tag="VFQ23_01880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9905350.1"
                     /translation="MIPTPSYEYYGMMAEFWDLFRGDTSTWEDRFFYLDVVKKHGQPA
                     LDVGCGTGRILLDFMQQGFDIDGVDNSPDMLALLRQKAEPMGLTPTVYQQEMTELALP
                     RQYQTILVPSSSFQLLLDANLPPVAIARFYEHLLPGGVLAMPFMTLWKEGDPLESEYT
                     REATRPADNALVRRWSHSRFDPDTELEHTIDRYEIVRDGEVTASEEHTQSPATRSYTQ
                     AQAISLYENAGFENIQVFHEFTFEPARPEDTIFCVLGFKPK"
     gene            1223..1708
                     /locus_tag="VFQ23_01885"
     CDS             1223..1708
                     /locus_tag="VFQ23_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HET9905351.1"
                     /translation="MFEQSTGSPHHFLSQLAGGWSGTSRVWIEPDNLADDSPVVGTIQ
                     LLLDGRFALYLYQGSIQGEPQHGMFTFGYNTTLDQYEASWVDSFHNNTAIMFCTGSLR
                     ENGFFVLGSFPDPNGGPDWGWRTEVELLDADRLLITAYNIDPEGGEAKATEARLTRVK
                     K"
     gene            1766..2758
                     /locus_tag="VFQ23_01890"
     CDS             1766..2758
                     /locus_tag="VFQ23_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9905352.1"
                     /translation="MKILIIGGTRFLGRHLVDSALARGHEVTLFNRGKSNPDLFLNIE
                     TILGDREHDLEKLSGREWDAVIDTCGYFPRIVRLSAKSLERSVGRYVFISSISVYAGY
                     SKIGIDENDPVGKIEDESFEEITGESYGPLKALCEKAVQEIYNERALIIRPGLIVGPN
                     DPTDRFTYWPVRVARGGDVLAPEKPEVPIQIIDVRDLSEFILKLIEDNASGVYNATGL
                     DYQLTLGRMLEVSKQVSKSDARFNWASVEILNQHKVEAWSDMPVWVPDTEEDAGFSRV
                     DISKAMDDGLTFRPLEETIRDTLDWANTRPPDHQWRAGLTAEREAEVLAVLKEE"
     gene            2760..3281
                     /locus_tag="VFQ23_01895"
     CDS             2760..3281
                     /locus_tag="VFQ23_01895"
                     /inference="COORDINATES: protein motif:HMM:NF040152.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HET9905353.1"
                     /translation="MQIHDPRQQLDHMLRQTRMNLVAFSQMADTKAHILLSLSSVILS
                     LSLTRIADARFSLAIAGLDVFLLVTIFFALLTVIGKIKVFDRKKHSVHDDDYSPLFFG
                     NYGDVPYDEYAKDFERIMNDSNATYEIMVKDIYYAGKYLLQTKYRYIRLAYFYFFTGL
                     IVGTIIYFIQYVF"
     gene            3283..>4086
                     /locus_tag="VFQ23_01900"
     CDS             3283..>4086
                     /locus_tag="VFQ23_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET9905354.1"
                     /translation="MFNKILIANRGEIAIRIMRACKELGIKTVAVYSEADKNSQHVQV
                     ADEAIHIGDPSPKASYLNMDVLIRAALDSNADAVHPGYGFLSENASFAGKVDSAQLTF
                     IGPSPDSIRAMGDKAESKKMMKQAGVPTVPGMEGLESEEDFQKAAKEIGYPVLVKAAA
                     GGGGKGMRVVNEESELKESIESARREALNAFGDERLLIEKYISNAHHIEVQVFGDQHG
                     HLVHLFERECSVQRRHQKIIEETPSLLLTPELRAQMGDAAVAAAKAVGYY"
BASE COUNT         1041 a          982 c         1074 g          989 t
ORIGIN      
        1 cgtcatgggc ggggaacaag ccgcgaatgt attgctcacc atcaaacaag atcaacttca
       61 acgcgaaggc aaacctgcga tgaccaaaga agaagctgat gaattcaaac gtcccatttt
      121 ggagaagtat gaggatgaag gaaatcccta tcattcgaca gcgcgattat gggacgatgg
      181 tgtgatcgat cctgtagata cgaggcaggt gttgggacta gcattatcgg ttgttttgaa
      241 ttcgccagtg gaagaaaata attttggagt ttttaggatg taatgattta gtgtcattgc
      301 gagccaccgc tggtcgagtg acacgaagtg gcgtatcgag accagtgtgg cgtggcaatc
      361 tcaagtttgg tggttagtcg agattgcttc ccgaaggctc gcaatgacat aatgaatagg
      421 aaattttatg atacccactc cctcctacga atactacggc atgatggcgg aattctggga
      481 tctgttccgc ggcgatacat ccacctggga agatcgtttc ttctatttgg atgtcgttaa
      541 aaagcatggt cagcctgcgc tggatgtcgg ttgtggaacc gggcgcatcc tgctggactt
      601 tatgcagcaa ggctttgata tcgacggcgt ggacaactcg cctgacatgc tcgcactgct
      661 tcgccagaag gctgaaccga tgggtttgac tccaacggtc taccagcagg aaatgaccga
      721 acttgcctta ccgcgccaat atcagacgat ccttgtgcca tcgagctcat ttcaactttt
      781 gttggatgca aacctgccac cggttgcgat agctcgcttt tacgaacatc tgctacccgg
      841 tggcgtgctt gccatgccct ttatgactct ttggaaagag ggcgacccgc tggaaagtga
      901 atacacacgc gaagcgactc gccccgcaga taatgcgctc gtgcgcagat ggtcccattc
      961 gcgctttgat cctgatactg aactcgaaca cacaattgat cgctatgaga ttgtccgtga
     1021 tggtgaggtg acggcgtctg aagaacacac gcaatcaccc gccacgcgtt cctatacaca
     1081 agcacaggca atctctctct acgaaaatgc aggcttcgag aatattcagg tctttcatga
     1141 gttcacgttt gaacctgcac gacctgaaga tacgatattt tgtgttttgg ggtttaagcc
     1201 caaataaagg aggtagtcac gtatgtttga acaatcaacg gggagcccac atcattttct
     1261 ttcccagctt gcaggtggct ggagtggcac atcccgagtc tggatcgagc cagataatct
     1321 ggctgacgac tcgcccgtcg taggcacgat ccagcttctc cttgatggac gcttcgcact
     1381 ctatctgtat caaggttcga ttcaaggcga gccacagcat gggatgttca cttttggcta
     1441 taacacgact ctcgaccaat acgaagccag ttgggtggat tcgttccaca acaacaccgc
     1501 tatcatgttc tgtacaggct ctttaagaga gaacggattc tttgtccttg gcagttttcc
     1561 tgatcccaac ggcgggccgg actggggatg gcgcacagaa gttgaattgt tggatgcaga
     1621 tcgcctcctt atcactgcct acaatatcga cccggaaggc ggcgaagcca aagcaaccga
     1681 agccaggttg acgagagtga agaagtgacc ctcgcgctga ggggagccgt gggcgcagac
     1741 gaagcgcgat aaaaatggag acacaatgaa aatccttatc attggtggta cacgttttct
     1801 tggaagacac ctcgtagact ccgccctcgc gcgcggacac gaagtgactt tgttcaaccg
     1861 cggcaagtcg aatccggacc tgtttctgaa tatcgagaca attctcggcg accgtgaaca
     1921 tgacctcgag aaattatccg gaagggaatg ggatgcggtc attgacacct gcgggtattt
     1981 tccgcgcatc gtccgcctct cagccaaaag cctggaaaga agtgtagggc gatatgtatt
     2041 tatctcttcc atttcagtct acgctggcta cagcaagatc ggcatcgatg aaaatgatcc
     2101 tgtgggcaaa attgaagacg aatcgtttga agaaattacg ggggaatcct acggtccatt
     2161 gaaggcgctc tgtgaaaagg cggtgcagga gatttacaac gaacgagcgc tgatcatccg
     2221 cccgggattg atcgtgggcc cgaatgatcc cacagaccga ttcacgtatt ggcccgtgcg
     2281 cgtggcgcgc ggcggagatg tacttgcacc ggaaaaacct gaggtgccga ttcaaatcat
     2341 tgatgtacga gacctttctg agttcatcct caaattgatc gaagacaatg cctctggtgt
     2401 ttacaacgcg acgggactcg attaccaact cacgctgggc aggatgcttg aggtcagtaa
     2461 acaagtgagc aagagtgatg caaggttcaa ctgggcaagt gtggagattt tgaatcaaca
     2521 caaggtcgaa gcctggagcg atatgcctgt ctgggttcca gatacagaag aggatgcagg
     2581 attctcgcgc gtagatatct caaaagcgat ggacgacggt ctgacgtttc gccccctgga
     2641 agaaaccata cgcgacaccc tcgattgggc gaacacgcgc cctccagatc atcagtggag
     2701 agcgggacta accgcagaaa gggaagcaga agtgcttgct gttttaaagg aagagtaata
     2761 tgcaaatcca tgatcctcgc cagcagcttg atcacatgct gcgtcaaacg cgcatgaacc
     2821 tggttgcgtt cagccagatg gctgatacaa aagcacacat cctgctcagt ctttcatcgg
     2881 tcatcctatc tctttctctc actcggattg ctgacgcgcg tttttctctg gcgatcgccg
     2941 ggttggatgt attcttactt gtcaccattt tctttgccct gttaactgtg atcggaaaga
     3001 taaaggtgtt tgatcgcaag aaacattctg ttcatgatga tgattacagc ccactctttt
     3061 ttggtaacta tggtgatgtt ccctatgatg aatacgcaaa agactttgaa aggatcatga
     3121 acgattcgaa tgctacgtac gaaatcatgg tcaaagacat ttactacgct ggcaaatatc
     3181 tgcttcaaac caagtaccga tatattcgcc tggcctattt ctattttttc acaggcttga
     3241 tcgtcggcac aataatctat ttcattcagt atgttttcta agatgttcaa caaaattctg
     3301 attgccaacc gcggtgagat tgccattcgc atcatgcgag cctgcaaaga gcttggcatt
     3361 aaaaccgtcg cggtctattc agaggcggat aaaaattccc agcatgttca agttgccgat
     3421 gaagcgattc atatcggtga cccctcaccg aaagcctcct atttgaatat ggacgtcttg
     3481 atccgcgccg cgctcgactc aaatgcggat gccgttcacc cgggctatgg attcctttca
     3541 gagaacgcct cgttcgcagg gaaagtggac tctgctcaat tgacttttat cggcccctca
     3601 cccgactcta tccgtgccat gggtgataaa gccgagtcta agaagatgat gaagcaagcc
     3661 ggcgtgccga ccgtgcccgg catggaaggt cttgaatcag aagaagattt ccaaaaggct
     3721 gcaaaagaga ttggttatcc cgtcctggtt aaagcggcgg cgggcggtgg cgggaaaggg
     3781 atgcgggtcg tcaacgagga aagcgagttg aaggaatcca ttgaaagcgc gcggcgtgaa
     3841 gcgctgaatg cctttggaga tgaacgatta ttgattgaaa aatatatctc gaacgcccat
     3901 cacattgagg tccaggtctt cggcgaccag cacggacatc tggttcattt attcgagcgc
     3961 gagtgttccg tgcagcgcag gcatcagaag atcatcgaag agacgccttc gctcttattg
     4021 actcccgagc tccgcgcgca aatgggggat gcggctgtgg cagcggcaaa agcagtgggt
     4081 tattac
//
LOCUS       DASTJP010000062         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1096449, whole genome shotgun sequence.
ACCESSION   DASTJP010000062 DASTJP010000000
VERSION     DASTJP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096449"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFQ23_01905"
     CDS             <1..666
                     /locus_tag="VFQ23_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HET9905355.1"
                     /translation="RKPGQPIIAPIPSIRSFIAGDMIFGIGIAILLVGFSIWGVSRVL
                     TIQSQQEVQPTAPSISDVLLASPEASEFTATSEALPAQIPGEATVTIVIPTQNLDVNV
                     QVNLVAVERTYMRVIVDGEEVFNGRVVPGTAYPYQAETQIEVLVGSGAAIRTVYNGRD
                     LGLLGTFGQVVNNIYLAEEIITPTALPSPEVTTTPPVTLTVSPTSTVTRTATPAPSNT
                     SSP"
     gene            707..2053
                     /gene="rimO"
                     /locus_tag="VFQ23_01910"
     CDS             707..2053
                     /gene="rimO"
                     /locus_tag="VFQ23_01910"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET9905356.1"
                     /translation="MSKNTFHLVSLGCAKNTVDSDSMAQLLLRDGYRAVDDPSRANVL
                     IVNTCGFIGPAKEESINVLRELADGKRNNQILIAAGCLTQRYGSEVAQKVPGIDGVLG
                     TRRWMDIVQVVRELRKGPHPEPLYHLPEAKTVGMDEQNALRASVAGASAYLKIADGCR
                     RPCAFCAIPLIKGTAVSRPVDLIIDEARRLRDAGVRELILIAQDTTDYGHDLGMKNGL
                     AILLEQLTASVPDMDWIRIMYAFPGYVTDRLIEVMAAHKQVLPYLDMPLQHAHPKTLY
                     RMRRPSNIDWVHRTLGKMRAAIPNLAIRTTFIVGYPGETEEEYQALVDFVQEIRFDRV
                     GTFQFSFEPGTTSEPLGDPVPADVKQARFEKLMELQQGISMQINQSYVGKTLDVLVEG
                     RDKGITIGRSYRDAPEIDGMVFVEGDARIGEIVPVKITGAMAYDLTGVLAPELIKL"
BASE COUNT          496 a          537 c          556 g          511 t
ORIGIN      
        1 cgcaagcctg gtcagcccat tatcgcaccc attccctcga tacgaagttt tatcgcaggc
       61 gatatgattt ttggcatcgg catcgccatc ctgttagtgg gattttcaat ctggggagtg
      121 agccgcgtat taacaatcca aagccagcaa gaggtccaac caacagcccc gtcaatttca
      181 gatgtattgc tggcatcacc cgaggcgtcc gaatttacag ccacctccga agcgttgccc
      241 gctcaaattc ccggagaggc tactgtgacg atcgtcatcc cgactcagaa cctggatgtg
      301 aatgtccagg tcaatctggt tgcggtggaa cgcacttaca tgcgtgtgat tgtagatggg
      361 gaagaggttt tcaatggacg agtggtgcct ggaactgcct atccctatca ggctgagacc
      421 caaattgaag tgttggtagg cagtggcgct gccatccgaa ctgtttataa tggacgtgat
      481 ctcggactcc tgggtacatt tggtcaggtt gtgaacaata tatatttagc cgaagaaatc
      541 atcacaccca ctgcattgcc ttcaccggaa gtaaccacca ctccaccagt gaccctgacc
      601 gtctcgccca cgtccacagt cacacgcaca gctacgcctg caccgtctaa cacttcttca
      661 ccgtagattc gctctccgtg acggagtcat aataaggaaa tttatcttgt ctaaaaatac
      721 gtttcatctt gtatcccttg gttgtgccaa aaacacggtt gactcagact caatggcaca
      781 actccttctc cgtgatggat atcgcgcggt ggacgaccca tctcgcgcga acgttctcat
      841 tgtgaatacc tgtgggttta ttggtcccgc aaaggaagaa tcaatcaacg tcctgcgtga
      901 attagcagat ggaaaacgca ataaccaaat tctgattgcg gcgggctgtc ttacacagcg
      961 ctatggttct gaagtcgcgc aaaaggtgcc cggaattgac ggcgtcctgg gtacgcgccg
     1021 ctggatggat attgtgcagg ttgtgcgtga acttcgcaaa ggtccacacc ccgaaccact
     1081 ttatcatttg cccgaagcaa agaccgttgg gatggatgag caaaatgctc tgcgggcgtc
     1141 ggttgccgga gcaagcgcct atctcaaaat tgcagatggg tgccgccgcc catgcgcctt
     1201 ctgtgcaatt ccgctgatta aggggaccgc ggtatcacgt ccggtggacc tcatcattga
     1261 tgaggcgcgt cgcctacgtg atgctggcgt gcgcgagttg atcctgattg cacaagatac
     1321 tacagattac ggtcatgatt tgggcatgaa gaacggtctg gccattcttt tggagcagct
     1381 tacggcatct gtgccagaca tggactggat ccgcataatg tacgcgtttc ccggttacgt
     1441 cacagaccgt ctgatcgagg tcatggcggc tcataaacaa gtgctgcctt atctcgatat
     1501 gcccctccag catgcgcatc caaaaacgct gtatcgcatg cgaagacctt ccaatatcga
     1561 ctgggtccat cgcacccttg gcaaaatgcg cgcggcgatc ccgaacctcg ctattcgcac
     1621 gacttttatt gtcggctatc ccggcgagac ggaggaagaa tatcaggccc ttgtggactt
     1681 tgtacaggaa atccgctttg atcgggtggg cacattccag ttctcgttcg agcctgggac
     1741 aaccagtgaa ccattaggtg acccggttcc tgctgatgtg aagcaggcgc gctttgagaa
     1801 actaatggag ttacagcagg gcatttccat gcagatcaat cagagctatg tagggaagac
     1861 gctggatgtg ttggtcgagg gacgcgataa agggattacg atcggtcgct cttatcggga
     1921 tgcaccggag atcgatggaa tggtcttcgt cgaaggtgat gccaggatcg gtgaaatcgt
     1981 gccggtgaag attaccggtg ccatggcgta tgaccttaca ggcgttctgg ccccagaact
     2041 gataaagttg taagtgttcg attaggacat tgggttcttg caaatttgac gattcataac
//
LOCUS       DASTJP010000063         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1163395, whole genome shotgun sequence.
ACCESSION   DASTJP010000063 DASTJP010000000
VERSION     DASTJP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163395"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1354
                     /locus_tag="VFQ23_01915"
     CDS             191..1354
                     /locus_tag="VFQ23_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9905357.1"
                     /translation="MLKQAHAISEELIEWRRDFHMHPEIGFEVHRTAGIVADELEKMG
                     YRVKRGVGKTGVVAEIGDEGKVVAIRADMDALPILEMNDSEYVSQNKGAMHACGHDSH
                     TAMALGAALLLSKEKLPGRVRFLFQPCEETADEEGKSGAQRMSAEGAMEGVDYVIAQH
                     VDPGMSVGTIGINAGPSSGGVDSWFAEIKGKGGHGAHPDKTVDPFFLLAHVILALNGI
                     VSRKLNPFEPAVVSIGSISGGFTQNVIPESVTITGTLRYTAESVQHQIHAEIKRALEV
                     AKALGGDYELKIEIGGPPMVNDELVSDMIEQTGKDLLGIENVQAIEKTLGAEDFGEFM
                     KHAPGAMFTLGTKKQGHEEYLLHHPKFDLDERAMPIGTAMLVETALRFLKNGN"
     gene            1474..1860
                     /locus_tag="VFQ23_01920"
     CDS             1474..1860
                     /locus_tag="VFQ23_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905358.1"
                     /translation="MLNHQFVFSGYLLFTFFLYVCMGYSYYLNSQRPTGDPKKLDIHI
                     GAVLLIPIAWPLLIVGAISLLIMKVLIYSVFLVLFTIALLLFRKPFVLDWLKKTAAWI
                     GDRLLEAHLFLIKIVFGSQSKKTQTT"
     gene            2146..3162
                     /locus_tag="VFQ23_01925"
     CDS             2146..3162
                     /locus_tag="VFQ23_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9905359.1"
                     /translation="MEALQQLGISLIQALQTLSPALDVFMYFFTFLGRIEFYLMIAPF
                     IYWTVDKRLGFRALLILVATDVVTSSFKLLLHQPRPYWIGDVQQLSQEATYGIPSSHA
                     SDSLAVSGYLAYRIRKPWLWVVIGLVVFFIGLSRLYLGMHFPHDVLVGWLIGAVVLWA
                     GINRSNQIATWARSKSLSAQIGIGFALSVFVILLSILIRFFISGTPDPESWSRFSTES
                     RSITHSFTLAGALFGSITGYALMRQYAPFLPGKTWIKRLLGYVIGFIVLLFIYEGLDV
                     AFSAIAADETILGYLLRYIRYTLMTIWAMFGAPWVFLKTGLAEAESGSTDANQRLRSK
                     SSIA"
     gene            3122..>3549
                     /locus_tag="VFQ23_01930"
     CDS             3122..>3549
                     /locus_tag="VFQ23_01930"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HET9905360.1"
                     /translation="MRTRDSEANPQSHRITSIDFLRGVVMVIMALDHVRMYFGYGTWY
                     AEPTNLSTTTPFLFFTRWITHFCAPVFVFLTGTSAYLYGLNKAKTSQLALFLCTRGLW
                     LVFAELIIVNFAWTFDTTFSFRILQIIWAIGISLIILSALV"
BASE COUNT          892 a          836 c          834 g          987 t
ORIGIN      
        1 gatgccttcc ttcgagtcac tcctcaagga aacaggaagg ctcgtaatgc aaacgttcgc
       61 cgcttagttg ttcctcttgt tcttcccgga acattttgag accaacaatc gaacaagctg
      121 taaaatagaa gctctggtac ctttagtact ctcgcccgga acaccaatca ccatcttaaa
      181 ggagaacgct atgcttaaac aagcacatgc aatttcagaa gaactcattg aatggcggcg
      241 cgatttccat atgcatcccg aaatcggatt tgaagttcat cgcactgcag ggatcgtggc
      301 ggatgaattg gaaaagatgg gatacagggt taagcgcggc gtcggaaaga ccggtgttgt
      361 ggcggagatc ggcgatgagg gcaaagtagt cgccatccgc gcggacatgg atgcgcttcc
      421 tattttggaa atgaatgact cggaatatgt ctctcagaac aaaggcgcca tgcacgcctg
      481 tggccacgat tcgcacacgg ctatggcact tggcgcagcc ttgttattat caaaggaaaa
      541 gctacccggt cgtgttcgtt ttctcttcca accctgtgaa gaaacagccg atgaagaagg
      601 caaaagcggc gcgcaacgca tgtcggcaga aggcgccatg gagggtgtgg attacgttat
      661 cgctcagcat gttgacccag gcatgtctgt cggcacgatc ggcatcaatg ctgggcccag
      721 ttctggtggc gtagattcat ggtttgctga gatcaaaggg aaaggcggtc atggagcgca
      781 tcctgataaa acggttgacc cgttcttcct gcttgcacac gtgatcctgg cgttgaatgg
      841 catcgtttca cgtaagctaa atccatttga accggctgta gtaagcatcg ggtcgatcag
      901 tggaggcttt actcaaaatg tcatccccga aagcgtgaca atcactggca ccctgcgata
      961 taccgcggaa agtgtacaac atcaaatcca tgctgagatc aaacgtgcgc ttgaagtggc
     1021 aaaagcgctg ggtggagatt atgaacttaa gatcgaaatt ggcggtccac ccatggtaaa
     1081 cgatgaactg gtctccgaca tgatcgaaca gaccggcaag gacttgcttg gcatagaaaa
     1141 tgttcaggca attgagaaga ccctgggtgc cgaagacttc ggagaattca tgaagcatgc
     1201 acccggtgcc atgttcacct tagggacaaa aaagcaaggg catgaagaat atctgctcca
     1261 tcacccgaaa tttgaccttg atgaacgcgc catgccgatt ggcacggcaa tgctcgtgga
     1321 aacagccctg cgatttctga aaaacggcaa ctaagacccg taaatctctt aataaagcca
     1381 ttctgtatca aaatgcagga tggcttttat ttgtacacaa aatgagataa atgttataaa
     1441 atatcttata acaaattact tcggggcaag ttcatgctaa accatcaatt cgttttttca
     1501 ggctatcttc tgttcacatt tttcttgtat gtatgcatgg ggtattctta ttatcttaat
     1561 tcccaacgtc ctaccggtga cccgaaaaag ctcgatatcc atatcggagc tgtgctactt
     1621 attcccatag cgtggcccct gctaatcgtt ggagcaatct ctctgctgat catgaaggta
     1681 ttgatctata gtgtttttct tgtccttttt acgattgcat tgctactatt ccggaagccg
     1741 tttgttttgg actggcttaa aaagacagca gcctggatcg gagacagact gctagaagcg
     1801 catcttttct taataaaaat tgttttcggc agtcaatcaa agaaaaccca aaccacataa
     1861 aagcccattt tttgatcaca gattcataat tttggttagc tgatctatct cgtctgcagg
     1921 ggataacttt ttcaagatag agtatcaccg ttgtaaaacc catagcttga aatagaaaaa
     1981 ctcatttcat ctcattatta atgaaaagta tcctgctcct tctgaaagcg atagcgacag
     2041 ggatcttttg atatgcagca attcccctac gattagtctt gaaattcagg gaaggatggg
     2101 ctatagtaat cacactgcta aacacgtctc agccaggagg tctccatgga agctttacag
     2161 cagttgggaa tcagcctgat ccaggcccta cagaccttaa gtcctgccct tgatgtattc
     2221 atgtattttt tcacttttct tggccgcatc gaattctatc tgatgatagc gccttttatc
     2281 tattggacag tggataaacg attgggcttc cgagcgctac tgatcctggt cgccacggat
     2341 gtggtcacat cgtcgttcaa gcttctgctt caccagcctc gaccttattg gatcggtgac
     2401 gtgcaacaac tatctcagga agccacgtac ggaatacctt catcacacgc cagcgactct
     2461 ctggcagtca gcggatatct tgcctaccgc ataagaaagc cctggttatg ggttgtgatc
     2521 ggcctggtcg ttttcttcat tggattatca cgcttatatc ttggcatgca ttttccacac
     2581 gacgttttag tgggctggtt gattggtgcg gtcgttttgt gggccgggat caatcgctca
     2641 aatcaaattg caacctgggc acgttcaaag tccttatcag cccagattgg aattggattt
     2701 gccttatctg tattcgttat cctgctttcc atactgatcc gcttctttat ctcagggacg
     2761 cccgatcctg agtcgtggag ccgtttctca accgaatcgc gttcgatcac ccattccttt
     2821 accctggcag gtgcattatt cggctcgatc actggctacg ctttgatgag acaatacgcg
     2881 ccttttttgc cgggcaagac atggatcaaa cgcctgctag ggtatgtgat tggctttatc
     2941 gtgctcctat tcatttatga gggtctggat gttgccttca gcgcgatcgc ggcagatgag
     3001 acgattttgg gatacttatt gcgttacatt cgctacaccc tgatgacaat ttgggcgatg
     3061 ttcggtgccc catgggtatt cctcaaaacc gggctggctg aagctgaatc aggatcaacg
     3121 gatgcgaacc agagactcag aagcaaatcc tcaatcgcat agaattacat caattgattt
     3181 tctgcgcggt gtcgtcatgg tcatcatggc gctggatcac gtccgcatgt atttcggtta
     3241 tgggacgtgg tacgccgagc caacgaatct ctctacgacc actccatttc tatttttcac
     3301 gcgctggatc acacattttt gcgcacccgt gtttgtcttt ctgacgggga cgtctgctta
     3361 cctgtatgga ctgaacaaag caaaaacatc tcaacttgcc ttgttcttat gcacgcgtgg
     3421 tctatggctg gtttttgcag aattaatcat cgtcaatttc gcgtggacgt ttgacaccac
     3481 atttagcttt cgcattctcc agattatttg ggcaattggt atcagcctga ttattttatc
     3541 tgcgctggt
//
LOCUS       DASTJP010000064         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_250585, whole genome shotgun sequence.
ACCESSION   DASTJP010000064 DASTJP010000000
VERSION     DASTJP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250585"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFQ23_01935"
     CDS             <1..321
                     /locus_tag="VFQ23_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET9905361.1"
                     /translation="PTTDTWSEGPSIEPRGTAGAVMYCDGIYLFGGESQAERKNLDSV
                     LRLDLGKNVWESVTPMPIARKFARAVLFRDSIYVVGGSTVLANSHSPIGTVSVVRYSQ
                     PGCS"
     gene            370..813
                     /locus_tag="VFQ23_01940"
     CDS             370..813
                     /locus_tag="VFQ23_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET9905362.1"
                     /translation="MRPNQKLIDKIRSFNRFYTNIIGLLDQHFLDSPFSLTEGRVLYE
                     ICNTEDCTAKKIRESFVIDEGYLSRILESFAKRGLIKKTPSRSDGRLRIILPTEKGKK
                     EFATLNDNSNKLVSEMIEKLTADEREDLLYRMEGIRKLLTKEHGR"
     gene            820..1815
                     /locus_tag="VFQ23_01945"
     CDS             820..1815
                     /locus_tag="VFQ23_01945"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905363.1"
                     /translation="MAIRSKTSADVFIRLAVVEEVEAIATVLREAFIEYESLYTPEGF
                     AATTPTSDQIRERWNEGPVWVAVQNEEIVGTVAAVSKGSGLYVRSMAVLPGAQGRGIA
                     RKLLDEIEHFAIHHHYDHLILNTTLFLKEAIHLYEQYGFQRLDEGKPDAFGRVLFTMV
                     KPLNRRISLEVISIRTHPKPGDLGYVIYLHGKRYGQEYGYGFQFERYVANGLCEFYEK
                     YDPARNRVWVCEHNDRMIGFLLLMDRGESAQLRYFLIEPEYRGIGLGSKLMNLYMDFL
                     RERGYQRSYLWTTHELITAANLYKRFGFQLTEEKESMSFGKPLKEQRYDLILSRG"
     gene            1846..>2466
                     /locus_tag="VFQ23_01950"
     CDS             1846..>2466
                     /locus_tag="VFQ23_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9905364.1"
                     /translation="MNTTPSDESIHLSAARVGNNEAFTALVEPHRKPLLVHCYRMSGS
                     LDDAEDLAQETFLRAWGKLKSFDGRGSFRNWLYVIATRLWLDEFRKRRKRILLPIDGD
                     PTDPDSPPQPDPSASFLEPLPASWLTSHDPTPEFSYERREIISLAFMVALQKLNARQR
                     VVLILREVFNWSAEEVAGALDLTVDSVNNLLYRARKNLEPSQMEENP"
BASE COUNT          661 a          594 c          601 g          610 t
ORIGIN      
        1 ccgactacag atacatggtc cgaggggcct tccattgaac caagaggtac agcgggcgcg
       61 gttatgtatt gcgatggcat ctatttgttc ggtggagaat ctcaggctga acgtaaaaat
      121 ctggatagcg ttctacgatt agacctggga aagaatgtct gggaatctgt cacgcccatg
      181 ccaatcgccc gcaaatttgc acgcgcggtt ttatttaggg attctattta tgttgtgggt
      241 ggaagtacgg tcttagccaa cagccattcg cccatcggaa ctgtttccgt agtgcgatac
      301 tcacaacccg gctgcagctg agtcttaaaa ctacttgaca aagtcaagca gtttgcttat
      361 tatggggcga tgagaccaaa ccagaagctc atagacaaaa tcagaagctt caaccgcttc
      421 tatacaaaca tcattgggct gttggatcag cattttcttg acagtccctt ctcactgaca
      481 gagggacgcg tgctctatga aatctgcaac acggaagact gcacagccaa aaagatcaga
      541 gaaagtttcg tcatcgacga gggctatctt agtcgtatct tggaaagttt tgcaaaacgt
      601 ggcttgataa aaaaaactcc ttctcgaagc gatggacgcc tgcgaatcat cctccccacc
      661 gaaaaaggga aaaaagaatt tgcaaccctg aatgacaact cgaacaaatt ggtctccgaa
      721 atgatcgaga agctgacggc agacgaacgt gaggatctcc tttacaggat ggagggaatt
      781 cgcaagttgt tgaccaaaga gcacgggagg tgaattgcaa tggcaatacg atccaaaact
      841 tcagcagacg tattcattcg ccttgccgtt gtcgaagagg tcgaggcgat tgctacagta
      901 ctgagggaag cgttcatcga gtatgagtcg ctgtataccc cagagggatt cgcagccact
      961 acacccacca gcgatcaaat ccgcgaacgc tggaacgagg gacccgtttg ggtagctgta
     1021 caaaacgagg agattgttgg aacggtcgcc gccgtttcga aaggttcagg cctttatgtc
     1081 cgaagtatgg ctgttcttcc cggtgcgcaa ggaagaggaa tcgcaagaaa actcttggat
     1141 gaaatcgaac attttgcgat acatcatcat tacgatcatc tcattctaaa cacaacactt
     1201 tttctcaaag aagcgattca tttatatgaa cagtatggat ttcaacgcct cgatgaagga
     1261 aagcctgatg cgtttgggag agtccttttt acgatggtga aacccttaaa tagacgcatc
     1321 tcgctggaag tgatttcaat tcgtacccac cccaaacctg gagatcttgg ctatgtgatc
     1381 tatctacacg gcaagcgata tgggcaggaa tatggctatg gattccagtt tgaaagatac
     1441 gtagccaatg gactatgcga gttttacgaa aaatatgacc ctgcaagaaa ccgtgtttgg
     1501 gtttgtgagc ataatgaccg catgatcggc tttctcctgc tcatggatcg cggggagtct
     1561 gcacagttgc gatattttct tatagaacca gaatatcgag gaattggact gggttcaaag
     1621 ctgatgaact tatatatgga ttttctgcgc gaacgtggct atcagcgctc ctacttatgg
     1681 accacgcatg aattgatcac agccgcaaat ctctacaaac gatttggttt ccagcttaca
     1741 gaagaaaaag aatcaatgtc gttcgggaag ccgctcaaag aacaacggta tgatttgatt
     1801 ctttcacggg ggtgaagcca aaatctgtta tagtatttca tccgtatgaa cacaacgcca
     1861 tcggacgaat ccattcatct gtccgccgct cgcgtgggga acaacgaagc ctttactgca
     1921 ctggtagagc cgcatcgtaa accattgctc gtgcattgct atcgtatgag cggctcgctc
     1981 gacgacgccg aagaccttgc gcaggaaact ttcctgcgcg cctggggcaa gctcaaatcc
     2041 tttgacggac gcggctcgtt tcgcaactgg ctctatgtca tcgccacgcg tctctggctg
     2101 gatgaatttc gcaaacgtcg gaagcggatt ttactcccaa ttgatggcga cccgaccgat
     2161 cccgattccc ctcctcaacc cgatccatcc gcgtcctttc tggagccatt gcctgcttcg
     2221 tggttaacca gtcacgaccc aacgcccgaa ttttcctacg aacgccgcga gatcatttcg
     2281 ctcgcgttca tggttgccct acaaaaattg aacgcgcgcc agcgtgtggt cttgatcctc
     2341 cgcgaagtct tcaattggag cgcggaggaa gttgctggcg cgcttgattt aacagttgat
     2401 tcggtcaaca atcttttata ccgtgcgcgt aaaaaccttg agccctctca aatggaagaa
     2461 aatcct
//
LOCUS       DASTJP010000065         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_250423, whole genome shotgun sequence.
ACCESSION   DASTJP010000065 DASTJP010000000
VERSION     DASTJP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250423"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="VFQ23_01955"
     CDS             <1..1474
                     /locus_tag="VFQ23_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9905365.1"
                     /translation="YGGRLENRVRFFRELIEDTKEAVGDTCAVAVRFAVDELLGEDGL
                     THQGEGYEVVAMLAELPDLWDVNVSGWNNDSIPSRFGKEGHQEKYISFVKQLTSKPVV
                     GVGRYTSPDSMVSAIQRGVMDFIGAARPSIADPFLPNKIKDGRVDDIRECIGCNICVT
                     GDTRFVPIRCTQNPTMGEEWRRGWHPEIISPKKSDKEVMIVGAGPAGLEAARALGQRG
                     YQVTLLEARKELGGRVLRESALPGLNEWRRVMDWRLTQIKKMKNVSMYPSSPMTAEEI
                     LESGAQHIILATGATWRRDGVGRTLWKPIPGYEFENVFSPDDLMNGKLLSGNITIYDD
                     DHYYIGGVLAELLATQGCNVTIVTPAPLVSYWSQYTLEQERIQQKLMKLGAKLYTQNV
                     VSKIENDCVHLINTISGDEVGLPGNGVVMVSDRIPNDSLYQSLKPALDEGKINSLRVI
                     GDAEAPNIIAQAVFAGHLAAREFDEREVEGTPFKIERIKL"
     gene            complement(1568..2911)
                     /locus_tag="VFQ23_01960"
     CDS             complement(1568..2911)
                     /locus_tag="VFQ23_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET9905366.1"
                     /translation="MSIFDKTRLTNETFKLDIDRMRRGWYSDKYFENIGRMLTTLASE
                     GYTYSGQKHNLPSTISPEDIAVGDIEVEMQWFTRRLGNTIVVGVDKSLEMLRHATGYW
                     KGDQFIETADKLEVWAVHDGAVVKSDGNPLNAESVLKVRGRYRDFALLETPTLGVLTR
                     SSRVATNVYETLTAARGKPVLFFPARFDLHEVQAADGYAYNIAIQRFNMDFSSQIGSF
                     VSTDAQGDWWGGAGGGTVAHAAIASFMGDTAEAMMQFSRILPAYIPRIALVDFNNDSV
                     RDTLRVIEAMFTRYRELRELGDEAEAAKYVLYGVRLDTSGTLRDVSVAPLGDPVLDLG
                     VNPRLVFNVRQALDSASESWSMPEAWKNRARDYCRSVKIVVSGGFNPEKIRKFEKLGV
                     PVDIYAVGSWLFNNNGGTVTDFTGDVVRVKVHDRWINMAKVGRMPLDNPNLEKVW"
     gene            complement(2944..>3138)
                     /locus_tag="VFQ23_01965"
     CDS             complement(2944..>3138)
                     /locus_tag="VFQ23_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905367.1"
                     /translation="SDLLEDIAMVDLELAEKVYLLEDCTSPVVVPGVVDHTDAGDAAF
                     ERFAKAGMNIIQSTQSVFGL"
BASE COUNT          783 a          793 c          835 g          727 t
ORIGIN      
        1 atatggcggg cgtttggaaa accgtgttcg tttctttcgc gagttaattg aagacaccaa
       61 agaagcagtg ggtgatacct gcgccgtggc ggtccgcttt gcagtggatg aattgcttgg
      121 cgaagatgga ctcacgcatc aaggcgaggg atatgaagtg gtggcgatgc tggctgagtt
      181 gccagatttg tgggatgtaa atgtaagtgg ctggaacaac gattcgatcc cgtcacgctt
      241 tggcaaagaa ggacatcagg aaaagtatat ttccttcgtc aagcagctga caagcaagcc
      301 ggtggttggc gttgggcgat atacctcgcc tgattcaatg gtctctgcca tccaacgcgg
      361 ggtcatggac tttatcggcg cggcgcgtcc ttcgattgca gacccctttc ttccaaacaa
      421 gatcaaagat gggcgtgtgg atgatattcg cgaatgcatt ggctgcaaca tctgtgtgac
      481 aggggatacg cgctttgtgc cgatccgctg tacgcagaac cccaccatgg gcgaagaatg
      541 gcggcgcggc tggcaccctg agattatttc accaaagaag agtgacaagg aagtgatgat
      601 cgtcggggcg ggaccggcag gtctggaagc ggcacgggcg ttgggtcaac gcggctatca
      661 agtcactttg ctcgaagctc gcaaagaact cggcgggcgt gtgctgcgcg aatctgcact
      721 acccggcttg aacgaatggc gacgcgtgat ggactggcga ttgactcaga tcaagaaaat
      781 gaagaatgtt tcaatgtatc catccagccc gatgaccgca gaggagattc tggaatctgg
      841 tgcgcaacat attattcttg ctacaggtgc aacatggcgg cgtgatgggg taggtcgcac
      901 attgtggaaa ccgattcccg gatatgagtt tgaaaatgtc tttagccccg atgatttaat
      961 gaatggtaaa ttgctgagtg ggaatatcac aatttatgat gacgatcatt actacatcgg
     1021 cggagtactc gcagaattac tggctacaca ggggtgtaac gtcacgattg tcacgcccgc
     1081 gccgctggtt tcttattggt ctcaatatac tcttgagcag gaaagaattc agcagaaatt
     1141 gatgaaactt ggcgcgaagt tatacactca gaatgtggtc agcaaaattg aaaacgactg
     1201 tgttcatttg atcaacacaa tttcgggtga tgaagttgga ttaccaggca atggcgtggt
     1261 aatggtgagc gaccgcatac caaatgattc gctgtatcaa tccctcaagc ccgctctcga
     1321 tgaaggaaaa ataaattcat tacgcgtcat cggcgatgcc gaagcgccaa atattatcgc
     1381 gcaggcggtg ttcgccggtc atctcgcggc gcgggaattt gatgaaagag aggtagaggg
     1441 aacgccgttc aagatagaaa gaataaaatt gtagggatgg gcgccctcat cagggtgctc
     1501 accggcactg cgcgcgggcg caagtgtcgc gagctgccta tccctacaat taattatgcc
     1561 ctgaagccta ccaaaccttt tccaagttcg ggttatccaa cggcattctc cccacctttg
     1621 ccatattgat ccatcgatcg tgcactttca cgcgtaccac gtcgcctgtg aaatctgtca
     1681 ctgtgccgcc gttattattg aacaaccagg aacccactgc atagatatct accgggacgc
     1741 ccagcttttc gaacttgcgg atcttttcgg ggttgaagcc acccgagaca acgatcttca
     1801 cactgcgaca atagtcacgc gcccgatttt tccaggcttc gggcatgctc cagctttcag
     1861 aggcggaatc caacgcctgc cgcacgttaa agacaagccg cggatttacg cccaggtcaa
     1921 ggaccgggtc gcccaacgga gcaacagaga catctcgcaa ggttccgctt gtatccagcc
     1981 tgacgccgta aagaacatat ttggcggctt ccgcttcatc gcccagctcg cgcagctctc
     2041 tatatcttgt gaacatcgct tcaatgactc gcagcgtatc acgcaccgag tcattgttga
     2101 aatccacaag cgcaatgcgt gggatgtagg caggcagaat ccgtgagaac tgcatcatgg
     2161 cttctgcggt atcccccatg aaggaggcaa tcgcggcgtg ggccacggtt ccgccgcccg
     2221 cgccacccca ccaatctccc tgcgcatctg tagaaacgaa ggaaccgatc tggctggaga
     2281 aatccatatt gaagcgttga atggcgatgt tataagcata gccatccgca gcctggacct
     2341 catgaaggtc aaagcgagcg gggaagaaca ggacgggttt gccgcgcgcc gcggtgagtg
     2401 tttcatagac gttcgtagcc acacgactcg aacgagtcag tacacctaag gttggtgtct
     2461 caagcaaagc aaaatcgcga tagcgtccgc gtactttcag aacagactct gcgttcaacg
     2521 ggttaccatc tgacttgaca accgccccat catgaactgc ccacacttcc agcttatctg
     2581 ctgtttcaat aaactgatcg cccttccaat agcctgtcgc atgcctgagc atttccagtg
     2641 acttatccac acccaccaca atcgtattcc ccaggcgacg ggtaaaccac tgcatctcca
     2701 cttcgatgtc acccacagcg atgtcctcag gcgatatcgt agaaggcagg ttgtgttttt
     2761 gcccagaata ggtatatccc tcagaggcaa gggtggtgag catacggccg atgttctcga
     2821 aatatttatc cgaataccag ccgcggcgca tacggtcaat atcgagtttg aaggtttcgt
     2881 tggtaaggcg tgttttatca aagatggaca tggattttat gatttcagat tagggatatt
     2941 ggattaaaga ccaaagactg attgcgtaga ctgaattata ttcattcccg cttttgcgaa
     3001 ccgctcaaaa gccgcatctc ctgcgtccgt gtggtcgacg acgccaggga ctaccaccgg
     3061 agaggtgcaa tcctccagca gataaacttt ctcggcaagt tcaaggtcca ccatggcgat
     3121 atcttcgagc aggtccga
//
LOCUS       DASTJP010000066        11185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1281260, whole genome shotgun sequence.
ACCESSION   DASTJP010000066 DASTJP010000000
VERSION     DASTJP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11185
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281260"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VFQ23_01970"
     CDS             <1..719
                     /locus_tag="VFQ23_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905368.1"
                     /translation="NEERNHNLTVASPKMIVQGQTIHVIWAAGSLPYRYYRFSNDAGQ
                     TWSAPEHVFGDLHGQAFDGLAVDRSGRVHFLGQVRYPMGIYHAYKDQTQWSKPALLYL
                     IAEIGSEDDFGDRVHAHNTIPVIRAGNQLVLTFGDPPSDPNRRLFVTYITLDDISPLQ
                     SAPTPILTATPIASSTPTPMQPTPMQSPTARSTLLLQTSNAQPVGPVPGPDLAIRVAL
                     VPTVLIIAFTMIIQLLKRRR"
     gene            complement(702..833)
                     /locus_tag="VFQ23_01975"
     CDS             complement(702..833)
                     /locus_tag="VFQ23_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905369.1"
                     /translation="MEAEMHNREAENDERRLPEKIILIQQKLSGIGQKEKVISAPAL"
     gene            855..2360
                     /locus_tag="VFQ23_01980"
     CDS             855..2360
                     /locus_tag="VFQ23_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905370.1"
                     /translation="MEYITASLTIDDTYYYLQTAWNAKSLGFVTFDGLHSTNGVQLLW
                     FVIVFMLAMLVKTKTALLFSALVVSFLLNSLCYLVILRIGVALKQPALILILASLWSM
                     QSIPFRIYSMGMENSLHALVFWCVIWQCIEFLIRVENRVNPNFLGLTVILILNAWTRI
                     DSALHSAILFAFCAGILAYPYRHNGRLFFQSYGKALASSIGLASLGFLAQLIAFRIMG
                     DSFLPVSALIKTSTTIAGAENQGIHNLVEVFLLGMPPVLQGRFADSFLVFLGISGILL
                     VILLGVIKQNQTVEFRTLLNLWSCFLVAEILYLVYVAFSGADYRPYFVWYRSPSFIFW
                     GISGSLLALFALPYAWLAKRSSHLLNQATIVFSLAIFAVAIYLFARGINFTSKLYTAR
                     YDAALWIADNSPPDTIFAAWNAGQLSFFSNRTFINLDGLINSIDYYERVLQGPVPLSD
                     YLVENKVEYIVDYAIYRPIPDYPVVQTFPLNDETGRSIYMWQVSSQLSLVP"
     gene            2742..3986
                     /locus_tag="VFQ23_01985"
     CDS             2742..3986
                     /locus_tag="VFQ23_01985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9905371.1"
                     /translation="MRILFLSNFFPPARPGGYTQWCHEVAERLAEHRHSVGVLTSRYE
                     REKAPPSEQNVYRILHLEGDLSYYRPLRFFTRWKKQHRENLAFLEQTVKDFAPDLIFV
                     WGMWALSKALPALAEQLLPGRVVYYLSDYWPSAMDMHTTYWQSPTRRGPMEVPKRVLS
                     KAAMSMLAKEDQPDLKLEHVICVSARVREILVEAGLPIQHATVIHGGTDVERFLSVRK
                     RDFRSGKLKLCYAGQLVWHKGVHTAIEAMAILVNQHRINQITLTVVGSGHPDYEVYLR
                     SLVEKEGLHPFVTFHKPVSKSEMPALLEQFDGLIFPSIYEEPLARMTQEAMASGLVVV
                     GTTTGGTKEILRDGETGLTFAPEDADGLAEQITQLIRNPDLGFRLAQAGHQTVLESFT
                     LDRMAQQIEAYLLACFARSSKV"
     gene            4011..5309
                     /locus_tag="VFQ23_01990"
     CDS             4011..5309
                     /locus_tag="VFQ23_01990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9905372.1"
                     /translation="MRILFLTNFYMVQETGGEDQSCQQVVQGLRERGHTTLVLTSMHG
                     TNNVPVQTNGVYRSLYLEMDLLPFRHSLVFFTQRKVRERHNLRYFEHVLKQFAPDIVF
                     IWGMWNLPRSLAALAESLFPDRVVYRFASYWPTLPSQHDSYWRAPGRNWYGKLVKKVL
                     GHVALAMLAGENQQPHLTFRNSICVSAMTRNRLVESGVPVANARVIHTGIDAKSYSAK
                     QHNPNTRAEPQTLNLLYAGRLVAEKGIETAIYAMEKLVINQGMRHVRLSIAGSGSADY
                     RSYLHSLVIQAGLDNFVSFLGHIPPEEMPRLLQESELLLVPSTWEEPFSRMILEGMIS
                     GVAVVATPTGGTTEIIKDGENGSLFTPGDAKDLARKIADLLNDPALRQRLAKAGKQTV
                     LERYTIAAMLDQVESFLHEVSGISMPERINHAFNVDQIAN"
     gene            5381..6385
                     /locus_tag="VFQ23_01995"
     CDS             5381..6385
                     /locus_tag="VFQ23_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9905373.1"
                     /translation="MEWTPTVSVIIPTYNRKDSLLRTLDSLKRQSFPAERMEVIVVDD
                     GSSDDTPTISSQKFPFTFRYLRQKNQGATAARNYGAQTSQSEILIFIDDDVIPSAQTL
                     EALAEVYRQETQVLATGTLIRCSGGQPSVYASIMLALESHIRTTKGDVELHHLNCNTE
                     LLACKRSDFFDLGMLQDPTDGHGWPNWDDVDFGYRAHLKGFRLLQIGKASGEHWDYSI
                     SDRTVACKRWYRASKSAVWLFKKHHPLQTLIPMLNDKTPLAWGQDSLFLIARKLARSV
                     VSSQLIVGWMEKFVGVLERHYPSPALLLHFYYWLQGAYMYQGYREGLREFELEGFRPS
                     "
     gene            6382..7296
                     /locus_tag="VFQ23_02000"
     CDS             6382..7296
                     /locus_tag="VFQ23_02000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9905374.1"
                     /translation="MMLSIIIVSWNTRQDLLRCLDSIMAAPPTYPFEILVFDNASEDG
                     SAEAVVTSHPGVSIEVSHQNLGFGRANNRAASSAQGRFWLLLNPDTIVHEGAIDTLVR
                     YLEGQPQVAAVGPRLVNADGSLQPSIERLPTLYNEWWRLLHLERLYSITQYPQSKMNS
                     RLPQIVEVLSGACLLLRRESVEIGGLFDEEYFMYSEEIDLCDRLHQDGWELHWVPEAV
                     VTHIGGQSTRQVALPMFIELYRNKVKFFRKRRGRVAAWFYKLILFQAAFIRYMLGNLI
                     QILPVRGRKELIEVCHRYGLLLIKLSSL"
     gene            7317..8510
                     /locus_tag="VFQ23_02005"
     CDS             7317..8510
                     /locus_tag="VFQ23_02005"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9905375.1"
                     /translation="MIVSLITLILILLPIAYWFMLALAAIRMPSRRSRLTRKPVTRFA
                     ITIPAHDEASVIGATVSRLRNLDYPSDMYAIHIVADHCTDETGRLAQEAGAVVHERQD
                     GPRTGKGAALAWLFERMLTEPYDAVVVFDSDTQVEPDFLRVMDARLAEESQVIQGQHI
                     IRNPESGWFPALTWAMFLIDNRFQNLGRSNLGWSAKNMGDSICFRADILRNLGWGEGL
                     TEDYQLRQRLLLAGIKISYEPRAKGYGEAPRTWAQARAQRARWLRGTQTASQQLARQL
                     LKAAIKHRDLAILDGALQAYLPSFSTLTVIGVLALLAQLLLNKVFLPPLIAPSLIYAW
                     IGVCVALFVYPLFGLALERAPARAYFVILTGPLFILWRTALAITSRYSRRPAIWVRTS
                     HGETK"
     gene            9029..10747
                     /locus_tag="VFQ23_02010"
     CDS             9029..10747
                     /locus_tag="VFQ23_02010"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET9905376.1"
                     /translation="MELNKYTFPLRKWWWLVAASTLIALIFSSLSALRQPAIYQARTT
                     LMIGTTINDPNPSSAELSLGQQLAAAYADLANREILRNATRNALGMNRLPAYFAWALP
                     NTQLIEITVNDTDRQRAQIVANELAAQLILLSPISGQTEEQERQEFIHERLNNLEIQI
                     QETESEIEKLQAELANTFSAQEINDKQSQISSLELKLNTLENNYGLLVSNTQQGATNT
                     LTIIAAAELPGSPIASMRGVTILLATVAGFVLAAGTAYLLEYMDDTLKSPDNVMRLFS
                     APILGRIFEQEGRMKESQFYDAENSNHPLTEPFRALRTEIDLAEMGQRLKTILVTSPD
                     IGDGKTSVAANLALSISQRGKKVFLLDADLRKPKIHKIFNLPNEEGLADVVFARAVFD
                     WRIGIKEFRQISVLTAGDTPPDPAELLSSEKMGLFLSELEQVADTIIIDGPPLFVPDA
                     MILASKVDGVLLVVRPTHTRQSLAKAAMEHIKLVGAKVVGVVLNRIPLRGADYYAGKS
                     YLFTYYSSNYGNENQASDSKNFGGSFFFSDIQNLWEISSPYINKVTTFFRQLPASLRS
                     RFQTKS"
BASE COUNT         2794 a         2631 c         2672 g         3088 t
ORIGIN      
        1 ataacgagga gcgtaatcac aatttgactg ttgccagccc gaaaatgatc gttcagggtc
       61 aaactataca cgttatctgg gctgctggca gcttgccata ccgttattat cgattttcaa
      121 acgatgctgg acaaacctgg agtgcaccag aacacgtttt tggcgactta catgggcagg
      181 cgtttgatgg tttagctgta gaccgatctg gccgggtaca tttcttgggc caagttcgct
      241 atcccatggg aatctatcat gcgtacaagg atcaaaccca gtggagcaaa cccgcactac
      301 tctacctgat tgcggaaatc ggatcagagg atgatttcgg cgatcgagtt catgctcata
      361 atacaatccc tgtaattcgc gctggtaatc aactggtcct tactttcggc gacccaccat
      421 cagatccgaa ccgccgttta ttcgttacgt acattaccct tgacgacatt tcgccgctcc
      481 agagtgcgcc aacgccgatt ctgacagcaa cgccaattgc aagttccacc cccacgccaa
      541 tgcaaccaac gccaatgcaa agtccgactg ccaggtcaac gctgctttta caaacatcaa
      601 atgcgcagcc ggtcggtcct gttcccgggc cagatctcgc tattcgtgtg gcattagttc
      661 caactgtgct catcatcgcc tttacgatga taatccagtt gttaaagcgc aggcgctgaa
      721 attacttttt ctttttggcc aatgccggag agcttctgtt gaatcaaaat aatcttttca
      781 ggaagtctgc gttcatcatt ctcagcttcg cggttgtgca tttcagcctc caacttagcc
      841 tccttaacaa aggcatggag tatatcacag catctctgac catagatgat acctattatt
      901 acttgcagac tgcatggaat gctaaatcgt tgggctttgt cacgtttgat ggtctccatt
      961 cgacaaatgg tgtacagctg ttatggtttg tgatcgtatt catgcttgcg atgcttgtaa
     1021 aaacgaaaac tgctctttta ttttcagcgt tggtcgtgag ctttttgcta aatagcctgt
     1081 gttatttagt tattttaaga atcggagtgg ctctcaaaca accggctctg atattaatac
     1141 tggctagttt atggtcgatg caaagcatcc cttttagaat ttattcaatg ggcatggaaa
     1201 attcattaca tgccctagta ttctggtgtg tgatatggca atgtattgag ttccttattc
     1261 gggttgagaa tcgggtcaat ccgaatttct taggattaac ggtcattctc attcttaacg
     1321 catggactcg catcgattca gcacttcact cagccatctt atttgctttt tgcgccggca
     1381 ttcttgcata tccctaccgc cacaacgggc gtttattttt tcagtcatat ggtaaagctt
     1441 tagcgagctc aattggactc gctagtcttg gatttcttgc acaattaatt gcttttcgaa
     1501 tcatggggga ttctttttta cctgtttccg cgttgatcaa gacaagcacc acaattgcgg
     1561 gcgcagaaaa ccaggggata cataacttag tggaggtctt cttattgggg atgcctcctg
     1621 ttcttcaggg tcggttcgca gacagttttc ttgttttcct tggaatctct gggatacttc
     1681 ttgtgattct cttaggagtg atcaaacaga atcaaacagt tgagttcaga actctactga
     1741 atctttggtc atgttttcta gttgcggaga tactttatct tgtttatgtt gctttttctg
     1801 gagctgatta tcggccatat tttgtctggt accgctcacc gtcatttatt ttctggggaa
     1861 tttcaggatc tctattggct ttgtttgctc ttccatatgc ttggctggca aaacgctcct
     1921 ctcacttgct taatcaagca acaattgttt ttagcctggc catcttcgcc gtagcaattt
     1981 atttgtttgc aaggggcata aactttacat caaaactgta taccgcaagg tatgatgcgg
     2041 ctctatggat agcagataac tctccaccgg atactatctt tgctgcttgg aatgcgggac
     2101 aactcagctt cttttccaat cggactttta tcaacttaga cggattgatc aacagtattg
     2161 attactatga gcgcgttctg caagggcccg ttccgctgtc cgattactta gtcgaaaata
     2221 aggtggagta tatcgttgat tatgcaattt atcgccccat tccggattat cctgtagtac
     2281 aaacgtttcc actaaacgat gaaacagggc gatcaattta catgtggcag gtatcatcgc
     2341 aactttccct ggttccctga tcaagattcg gtttgatgta tcccaaagct gtgattagat
     2401 cgaaatcgtg aaagtttggc atcagggtta agattttcca agtgtattaa cacgaagttt
     2461 tcctgtggaa acagtggtta tataggagaa aacagtgaag aatggttggc atttcgatct
     2521 ttggagcaac gtcgatacca gtttgctttt taaaagtagc gccgccgacc aatttgacct
     2581 ctggcggctg gtgcgccggg gttaagaggc aagaataata agagaacttg caacacctgt
     2641 ggctttaatt gctataaatc ctgattccct tactatttcc cgctcctgaa cgcagaagcg
     2701 ttcaggagcg ggagtgtcaa ggaagccaac ttctacacaa aatgcggatc ctttttctct
     2761 caaacttctt tccgccggcc cgtcctggcg gttacactca atggtgccat gaagtagcag
     2821 aacgtttggc tgagcatcgt cattcagtcg gcgtgttgac aagtcgctat gaacgggaga
     2881 aggcccctcc cagcgagcag aatgtttatc gaattcttca tcttgaggga gatctttctt
     2941 actatcggcc tctccgattt ttcactcgct ggaaaaaaca acaccgcgaa aaccttgcgt
     3001 ttctggaaca gaccgtgaag gacttcgcac cagatttgat atttgtctgg ggtatgtggg
     3061 ccctttcaaa agcactgcca gctctggccg agcagctact accaggtcgt gtggtttact
     3121 acctatctga ctattggcca tcggcaatgg atatgcacac tacctactgg cagtcaccaa
     3181 ctcgacgcgg tcctatggaa gtaccaaaac gagttctgag caaagctgcg atgtccatgc
     3241 ttgccaaaga ggatcaacca gatctaaaac tagagcacgt aatttgcgtc agtgccagag
     3301 tgcgggagat tctcgttgag gcaggtttgc ctattcagca tgccaccgtt atccatggcg
     3361 gtaccgatgt tgaaaggttc ttaagtgtcc gcaaacgcga ttttcgatct ggaaaattga
     3421 aattgtgcta tgctggccag ttagtgtggc ataaaggcgt ccacacggcg attgaagcga
     3481 tggcaatact ggtgaatcaa cacaggataa atcaaattac attgaccgtg gttggatcgg
     3541 gtcatcctga ttatgaagtt tacttgcgca gcctcgtcga aaaagagggg ctgcatccct
     3601 ttgtgacatt tcacaagcct gtttcaaaaa gtgaaatgcc tgccctctta gagcagttcg
     3661 acggtcttat ttttccgtca atctacgaag agcccttggc ccgcatgaca caggaagcca
     3721 tggcttcagg cctagtggtg gttggcacta ctactggtgg tacgaaggaa attttgaggg
     3781 acggcgaaac agggcttacg tttgcacccg aagacgccga tggcttggct gaacagatta
     3841 cgcagctaat cagaaatccg gacctgggtt ttcgcttggc gcaagccgga caccagaccg
     3901 tactggaaag tttcacccta gacaggatgg cgcaacaaat cgaagcttat cttttggctt
     3961 gttttgctag aagttcaaag gtgtagatta ttaattttag ccaaggaaaa atgcgaattc
     4021 tatttctcac caacttttat atggttcagg aaacgggagg agaagatcaa tcatgtcagc
     4081 aggtagtaca gggacttaga gaacgaggcc atactacact tgttttgacc agcatgcacg
     4141 gcaccaacaa cgttcctgtg caaacaaatg gagtctatcg gtccttgtat ttggaaatgg
     4201 acctgctacc ctttcggcac agtttagtct tctttacaca gcgaaaggta agggaacggc
     4261 ataatttgcg atacttcgag catgttctaa aacagtttgc gccagacatc gtctttatct
     4321 ggggcatgtg gaatttgcct cggtcactgg cggctttagc agagtccttg tttccagatc
     4381 gtgtcgtcta tcgatttgcc tcgtactggc ccacgttacc cagccaacac gattcttatt
     4441 ggcgcgcccc cggacgcaac tggtatggca agttggtgaa aaaggtttta ggccacgttg
     4501 ctcttgcgat gctggccggc gagaatcagc agcctcactt gacgtttagg aattctattt
     4561 gtgtcagcgc tatgactcga aatcggctcg tggaatctgg agtcccagtt gcgaatgctc
     4621 gtgttattca taccggtatt gatgccaaat catattcagc taaacagcat aaccctaaca
     4681 ccagggccga acctcagacg ttgaatctat tgtacgcggg tcggctggta gctgaaaagg
     4741 gtatagagac agccatttac gctatggaaa aactcgtaat taatcaaggc atgcgccacg
     4801 taagattgag tatagccggt tccggatctg ctgactaccg aagctatcta cattctctag
     4861 taattcaggc tgggcttgac aatttcgtgt cctttttggg acatatacca ccagaagaaa
     4921 tgccccggtt actacaagag tctgagttat tattagtacc ctccacgtgg gaggaaccct
     4981 tttcccggat gatattagaa ggcatgatct caggcgttgc agtggtggcc acccctacgg
     5041 gaggaacgac tgagatcatt aaggatggag aaaatggatc cctgttcaca cctggcgacg
     5101 ctaaggactt agctcgaaaa atcgctgatc tcttgaatga tcctgcgctc cggcaaaggc
     5161 tggcaaaagc aggtaagcag acagtgctgg agagatatac catagcagca atgctagatc
     5221 aggtagagag ctttctccac gaagtctctg gtatctctat gcctgaacga atcaatcatg
     5281 cattcaatgt tgatcagatt gcgaactgag ggttcttcgt ctcagacacg tattcaactg
     5341 tgagctaatt cgcgtatttc aaggaggcgt gattctaatt atggagtgga ctccaacagt
     5401 cagcgttatt attcctacct ataatcgcaa ggattcgctg ctacgcacac ttgatagttt
     5461 aaaacgacag tcatttcccg ctgaacggat ggaggtgatt gttgtagacg acggctcttc
     5521 ggatgatact ccgaccatct ccagccaaaa gtttcctttt acctttcggt atctgcggca
     5581 gaaaaatcaa ggagcaacgg ccgcacgaaa ttacggggcc cagactagcc aatctgaaat
     5641 tttgattttt attgatgatg atgtcattcc ttcggcgcag acgttagaag ccttggctga
     5701 agtgtatcgt caagagactc aagttcttgc gacgggaaca ttaatccgat gcagcggagg
     5761 tcagccctct gtgtatgcaa gtattatgct ggcactggag agtcatatac gcacgaccaa
     5821 aggtgatgtt gaattgcatc atcttaattg caacacagaa ctccttgcgt gtaaacgcag
     5881 tgactttttt gatcttggca tgttgcaaga ccctacagat gggcatggct ggccaaattg
     5941 ggatgatgtt gattttggat atcgagccca cttaaaaggg tttcggctgc tacaaattgg
     6001 caaggctagc ggggaacatt gggattattc catttctgat cgaactgtag cttgcaaacg
     6061 ttggtatcgg gcgagcaaat cagctgtttg gttgttcaaa aaacatcacc ctcttcaaac
     6121 tcttattcct atgctcaatg acaaaactcc tcttgcctgg ggacaggact ctctcttcct
     6181 catcgcccgg aagctggctc gttccgtcgt gtcctctcaa ctaatagtag gatggatgga
     6241 gaaatttgtc ggcgtcctgg aacggcatta tccttcccct gctttacttc tgcatttcta
     6301 ttattggctt cagggagcct atatgtatca aggatatcgc gaaggcttgc gagagttcga
     6361 attggaagga ttccggccat catgatgctt tcgatcatca ttgtcagttg gaatacgcgc
     6421 caagatttgc ttcgttgcct ggactcgatc atggccgccc caccgaccta tccctttgag
     6481 attcttgttt tcgacaacgc atcggaggat ggcagtgcgg aggcagttgt cacttcacat
     6541 cccggagtca gcatcgaggt cagccatcaa aatcttggct ttgggcgggc caacaaccga
     6601 gcggcgtcga gtgcgcaagg caggttttgg ttgttgctca atccggacac tattgtgcat
     6661 gaaggtgcca ttgacacttt ggtgcgctat ctagagggcc aaccccaggt cgcagctgtt
     6721 ggtcctcgtc tagtcaacgc agatggctcg ctccaaccct cgatagagcg tcttccgacg
     6781 ctctataacg aatggtggcg tctattgcat ttagagcgtc tttattcgat tacccagtat
     6841 ccacagtcga aaatgaactc ccgcctgcca caaattgttg aggttttatc gggtgcctgc
     6901 ctgctattga ggcgcgagtc cgttgagatt ggagggctgt ttgatgaaga atactttatg
     6961 tactcagaag agattgatct ttgcgaccgc ttgcatcaag acggatggga actccactgg
     7021 gtgccagagg ccgtcgtcac ccacataggc gggcaaagca ctcgccaggt ggctctaccg
     7081 atgttcattg aactgtaccg taacaaggtt aagttcttcc gaaaacggcg tgggcgggtt
     7141 gctgcctggt tctataaatt aatactattc caggccgcct tcatccgata catgcttggc
     7201 aacttgatac agattctacc cgttagaggt cgcaaagagt tgatagaagt ctgtcatcgg
     7261 tacggcttat tgctcatcaa gctttcaagt ttataaacac agttgtaaca ataaacatga
     7321 tcgtttcact gattacactc attcttattc ttctgcccat tgcatattgg ttcatgctcg
     7381 cgctggcggc gattcggatg ccatctcgcc gctccaggtt gacgcgaaag ccagtaactc
     7441 gatttgcaat cacaatcccc gcgcacgatg aagcctctgt tattggggca acagtcagta
     7501 gactgcgcaa cctcgactat ccgagtgaca tgtacgccat tcacattgtt gctgatcact
     7561 gcactgacga gaccgggagg ctggcacagg aagctggtgc ggtcgtgcat gaacgacagg
     7621 atggtccgcg gacgggcaaa ggtgctgcac tggcatggtt gttcgaacgg atgctaaccg
     7681 aaccgtacga cgccgtggtg gttttcgact cggatacgca ggtcgaacct gattttctac
     7741 gcgtgatgga tgcgagattg gccgaggagt cccaggtcat acagggccag catatcattc
     7801 gcaatcctga aagcggctgg tttccggccc taacctgggc tatgttctta attgacaacc
     7861 gttttcagaa tttgggtcga tccaatttag gatggtcagc caaaaacatg ggagactcga
     7921 tctgttttcg ggccgacatt ttgcggaact tgggctgggg agagggtctg accgaagact
     7981 atcagttgcg gcagcgtctc cttctggctg ggatcaagat cagttatgaa cctcgtgcta
     8041 agggctatgg agaggctccc cgcacgtggg ctcaggcccg ggcacaacgc gcgcgttggc
     8101 tgcgtggcac tcagacagcg agtcaacaat tggcgcgtca actcctcaag gctgccatta
     8161 aacatcgcga cttggccata ctggacggtg ccttgcaggc ctatctacca tccttttcaa
     8221 cgttgaccgt tattggtgtt ctagccctgc tggctcaact tttattgaac aaggtgttct
     8281 tgccgccgct gattgcgccc tctctgattt atgcatggat cggggtttgt gtagctctat
     8341 ttgtctatcc acttttcggc ttggccctgg aaagggcgcc tgcccgcgcc tatttcgtca
     8401 tcctgactgg tccactcttt attctttggc gaaccgctct ggccattacg tcgcgctatt
     8461 cccgtcggcc tgcgatctgg gtgcgtacgt ctcacggaga gaccaagtga ccatcgcacc
     8521 gccgccattc agcatgcctc aacccatcaa gagcgaaatt gccgattcga tatttttaca
     8581 aggtacacca cctcggtgat gtttgccggt gttattgccc cgactgtcac agtattaata
     8641 tgggcccatc gaggctggac tctgcaataa agccaaatgg cagtctcatt ttagagaacg
     8701 acaggctaga ggggttattc gttcgcatta tttttggctt tgtaagttct cccgtccttg
     8761 ttacaaaatc acgcgtagtg cgtcatgact acggtaaagc gcccagcagt tataacgaat
     8821 ctacagcccc tgaaacggaa ccgtaccagc ttgtcttccc tttgaccaat tatctgaaga
     8881 aacgtttaca atggatccaa cttgtcaaat tcaagaaaaa gaccaaagta ggtcggcagc
     8941 gtacaaaaat cgagacctta aagatgcaaa ttggcattag catgattaaa gaaacatcta
     9001 acagcttttt ttcaacgcgg gaatgtatat ggaactaaac aaatatacct tcccgttacg
     9061 caaatggtgg tggttagtgg ccgcctcaac tctaatcgct ttaattttca gctctctgag
     9121 tgcactgcgt cagcctgcta tttatcaagc tcgcaccacc ttgatgattg gaacgacaat
     9181 caacgatcca aatccttcca gtgccgagct atcgttgggc cagcaattgg ccgctgccta
     9241 cgccgatctt gccaatcgag agatactccg caatgcaacc aggaatgctt taggcatgaa
     9301 tcgattgccg gcgtattttg cgtgggcgct acctaacacg caattgatcg agatcaccgt
     9361 caacgacact gaccggcaac gggctcaaat cgtcgctaac gaactagccg ctcaactaat
     9421 ccttctaagt ccaattagtg gccagactga agaacaagag cgtcaggagt tcattcatga
     9481 acggttaaac aatctcgaga tccagatcca ggaaaccgag tctgaaattg agaaactaca
     9541 agcagagttg gccaatacat tcagcgcgca ggaaattaat gataaacaaa gtcagatttc
     9601 ttccttagaa ttaaagctaa ataccctgga aaataattat ggtttattgg tctccaacac
     9661 gcagcagggc gcgaccaata ccctcacgat tattgcagcg gctgaactac caggttcccc
     9721 cattgcatca atgcggggag taactatact gctggcaaca gtcgctggat ttgtcttagc
     9781 agctggtaca gcctatctgc tcgaatatat ggatgatact ctgaaatcac ctgacaatgt
     9841 gatgcgttta ttctcagctc ccattcttgg tcgtattttt gagcaggagg ggcgaatgaa
     9901 ggagagccaa ttctatgatg cagaaaattc taatcatccg ttaacggaac ctttccgcgc
     9961 tttaaggaca gaaatcgatt tagcagaaat gggtcaacgc ctgaagacaa tccttgttac
    10021 cagcccagat atcggggatg gaaaaacttc agttgcggcc aatctggcgc tgtcgatctc
    10081 tcaaagaggg aaaaaagtgt ttttgctcga cgctgacttg cgaaagccga aaatccataa
    10141 gattttcaac ttgccaaacg aggaaggtct ggcggatgtg gtgtttgccc gggccgtctt
    10201 cgattggcgg atcggaatta aggaatttcg gcaaatctct gtactgaccg ccggtgatac
    10261 ccctcctgac cctgctgaat tattgagttc ggagaaaatg ggactgttcc tgtctgagtt
    10321 ggaacaagtc gccgatacga ttattattga tggtccaccc ttatttgtac cggatgccat
    10381 gatcctggca tccaaggtcg atggggttct gttggtggtt agacccactc atacacgaca
    10441 atccctggcg aaagccgcta tggagcatat caagttggtc ggtgctaagg tggttggtgt
    10501 agtgctaaat cgcattcctc tccgtggtgc agattattat gctggcaaaa gttatctttt
    10561 tacttactac agcagtaact atgggaatga aaatcaagcc agtgatagca aaaacttcgg
    10621 cggatctttc ttcttttcag atatccagaa tttatgggaa atcagttccc cgtatataaa
    10681 taaagttacc acgttcttca ggcagctccc ggcatcgctt cgaagccgtt ttcaaactaa
    10741 gtcatagata acttaagcac tgatcttgtt ttaacaacac ggcaaggatc catattcctt
    10801 gccgtgcttt tttagtcata cttcagctat ttatttcgct tatcagcaga agtgaagtgg
    10861 ttacccatag gactgttatc cgatatttgt ataaaagcgg cggagtctgg tcacaacagc
    10921 acgaattaat acttaaaagc aaagaccacg aatttttcgt ggtctttgta gtagcggggg
    10981 caggacttga acctgcgacc ttcgggttat gagtggctct cggcgaccgc tgactttcga
    11041 gcgacaggct atgtgttttc aggcagcctt ttgatttttg attcaaaaga gcctgtagag
    11101 tttcgattgt cgcaatcgac aaccgtttga gcatttataa cacaaataga atatctaagt
    11161 caataaatga actccagatt acggg
//
LOCUS       DASTJP010000067         7207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1099765, whole genome shotgun sequence.
ACCESSION   DASTJP010000067 DASTJP010000000
VERSION     DASTJP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099765"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFQ23_02015"
     CDS             complement(<1..1012)
                     /locus_tag="VFQ23_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905377.1"
                     /translation="MSFLEPLRRIREKPWFYPGALLLMGMVTYNYALTALGYYWADWE
                     IMMFIKLNPALQFDYYAEDRPFPWTYQLIHFLVGANPIGWHVVTLLIRWGASLIFVNA
                     LTLIWPRYKNHFYWLGAILMVYPGFLQQLQSATKARHIMTFLLFALSVYLMALAIKRP
                     KWSRLLFPFSWLITLLHLFTTEYFAGLELVRPLLLWLLLDRENNKVIDSVRRVAIYYS
                     PYLIITLFFFWCRFIYFPSIFNTVSRIGDINVVVGGIQESTFDTLVALLNRVLFDLIY
                     LVVQVWTNAIIGLEGFSFQNKVAWFAFGLGILFSLAFAFFYNVDENEDGNQASPHSLL
                     LVG"
     gene            complement(1009..1533)
                     /locus_tag="VFQ23_02020"
     CDS             complement(1009..1533)
                     /locus_tag="VFQ23_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9905378.1"
                     /translation="MTFDPTFIGNLILVLTGFGGAFLAALWISLVIWTYRDIRTRARD
                     PLVQTLATLLVAVLNLPGVLVYLILRPPRTLEEEYQKTLEEEALLQALEDLPLCPGCE
                     RRVKDDWQVCPNCHTKLKKSCENCNRLMELPWNICPYCGTPAAGMRRDAVTSMDEALR
                     GLKLNDDPEGANIE"
     gene            complement(1566..2819)
                     /locus_tag="VFQ23_02025"
     CDS             complement(1566..2819)
                     /locus_tag="VFQ23_02025"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HET9905379.1"
                     /translation="MRFFSLNLVLCLVVLLSSCDIGVDDVPNSGIPTSTPFQPQPENL
                     SDSPYSAAAPTPLNLPTLTPLPNGPTEVVAASGSLPADFPTLISPPNLNPLTGLEPSD
                     LNLLNRRPMAIKVANYPRYIRPQSGLTLADNIYEYYIEDGLTRFIAVFYGNNSEWVGP
                     VRSGRYFDEHIQRMYQAYLVFKFADPREMNHFLLSDFYDFLVVPGTGSCPPFRLMESR
                     NIEVYNNSYFNTLLWEDCILENGLDNQRPQFRSGLFSDTVPQADLAGTQMFTDYSVDS
                     YHYWVYQPETQAYVRYQEVADTRDNKPEKYDVMVDAVTERPVTASNIVYLLASHTFTN
                     SFDEDDEVFKIDLTGSGEAYVFRDGVGLVARWYRLNPDQPLLLAGVNGAPIYLRPGVT
                     FYEVLGARSFVDQGDGEWHFHHDVP"
     gene            2920..4491
                     /locus_tag="VFQ23_02030"
     CDS             2920..4491
                     /locus_tag="VFQ23_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905380.1"
                     /translation="MLIFITILVFFLTALTLLILRFFAPQFRYSWVIATVGALAGWIS
                     VFVWQVQMPITLQFSIWQPSTLFLQSPALIADGISWAFAVSLAALCLAIIITSVVRAN
                     FPSPLTWVGSLVLTSFGILAVVADNPLTLVLIWAAIDLVELITQMRFVDNPRLSERVV
                     IAFASRAVGILTLLWADMVSAASGQVLGFRLASEASPQAGLFLVIAAGLRIGVLPLHL
                     PYASESSIRRGFGTGLRMVSAASSLILLARIPSSSVTSPFTPYLLILVSLAAVYGSWM
                     WLRAPDELTGRPYWLIGMGSLVIAAGLRANPVGAAAWGCGLILAGGALFLSSEQNRWL
                     TGALLIGALGISSLPFTLTATGWNSGGPSFWFTWPFLLVSQGMLIAGYIRHSRRTVMR
                     ESNEDQPIWAKNVYPFGIYLLVLMVILLGLYGWDGTLQFGNWLMGLIASVLTIGLLWL
                     MPRLRILNPVRAHWVRPVNTSWMEWSYQILWGLYRQLGKLSNAFTNMLEGESGIMWTL
                     LFLVLFISFFRQGSP"
     gene            4495..5100
                     /locus_tag="VFQ23_02035"
     CDS             4495..5100
                     /locus_tag="VFQ23_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905381.1"
                     /translation="MAEILPWIAVVVILITSVGLLLVRDWRLSIIFLAVQYLCMLIMV
                     LQHWPLGMATVKVVAGWMSAAILGMTRSGLASQGFDEESIWPRGRLFRLFAAGMVGVI
                     VSAVTPSVSTIMADAGFAVTSGSLLLIGMGLLHLGITTSILRVTIGLMTVLSGFEIIY
                     SAVEGSVLVAALLAIINLGLALTGSYLMIASSSSESEIESV"
     gene            5097..6494
                     /locus_tag="VFQ23_02040"
     CDS             5097..6494
                     /locus_tag="VFQ23_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9905382.1"
                     /translation="MNAPTIWIVVPLFFGILLLFINNQRALSILGGVFSASLALIAQF
                     VPIEQATLVGAASFKIDSSLTVLGRTLVILPAEGSLLALIYGATALWFFGAEVSKTAT
                     RIVPIGLMITALLVGSLAVEPFLFAALFIEMAILLAVPLLTSIYSPPGRGVVRFLIYQ
                     TLAMPFILLTGWLLAGVEASPGDLALAAQSSSMLGLGFAFLLAIFPLYNWVPMLMEET
                     SPYTAGFLLWILPTITIIFGAGFLDRYSWLRSSTELINALQFTGLLMIVTGGLFAGFQ
                     RHLGRIMAYGVIAETGFSLLALSLDPRLGIPILFLLIPSRALGQAVWALSLTVIKDQV
                     ETMRFRSSRGLLRVTPIAGAGMIVAALSTGAFPLLAGFPSRLALWEGLSRVSLGASVW
                     MGIGILGLLTSAFRSLAVISMAEEYTSWERRESLAQAIMLGVGMIGLFILGLFPQVIQ
                     YFLSGLPAMFENLGR"
     gene            6548..>7207
                     /locus_tag="VFQ23_02045"
     CDS             6548..>7207
                     /locus_tag="VFQ23_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905383.1"
                     /translation="MEYEQLVKRLEWVEGEHRKAKSTILTLEERIAALDSNIEVVAKQ
                     IKPLDKRIADVASTASRLDQFDAIFAKQREDMNKGLDDIEKRRQKQDNEIAKRHQQDL
                     EPLVKALSDLRKSMESEFPPIKRDIKARIIEETRLQKEIKELRPPIDEAVRIAHEATL
                     NQKVFEEARKQEAKRVTDLQGELTAIRVRIDEARAKADLNADGWRNMDNRIKELMASE
                     TE"
BASE COUNT         1736 a         1782 c         1853 g         1836 t
ORIGIN      
        1 gaccaaccag gagaagcgaa tgcggagatg cctgattgcc atcttcattt tcgtccacgt
       61 tataaaaaaa tgcaaaagca agcgaaaaca agatgcccag gccaaaagca aaccaggcta
      121 ctttgttctg aaatgaaaaa ccttcgagtc caataatcgc attggtccaa acctgcacaa
      181 ctaaataaat cagatcaaac aataccctgt ttaagagagc gactagagta tcaaacgtgg
      241 actcctgaat tccacctact actacgttga tatccccaat acggctcacc gtgttaaaaa
      301 tggatggaaa ataaatgaat cgacaccaga agaaaaatag ggtaattatt aagtacggcg
      361 aatagtagat tgcaacgcga cgaaccgagt cgatgacctt gttattctcg cggtcgagca
      421 gcaaccacaa aagcagtggg cgcactaatt cgagcccggc gaaatattcc gtggtgaata
      481 gatggaggag agtgatgagc caggaaaatg ggaagagcaa acgtgaccat tttggacgtt
      541 ttatcgccag cgccatcaga taaacagaca gcgcaaaaag taaaaacgtc atgatgtggc
      601 gcgccttcgt agctgattgc aactgctgaa gaaaacccgg ataaaccatc aagattgcgc
      661 caagccaata gaaatgattc ttgtaacggg gccaaatcag tgtcagggca ttcacaaaaa
      721 tcaagctggc gccccagcga atcagcagag tcacaacatg ccagccaatc ggatttgcac
      781 cgaccagaaa atggattaac tgatacgtcc agggaaatgg gcgatcctcc gcatagtagt
      841 caaactgtaa ggcgggattg agtttgataa acatcatgat ttcccaatcc gcccagtagt
      901 aaccaagggc cgtgagcgca tagttatacg tcaccatgcc cattaacaac aaagcgcctg
      961 gataaaacca gggcttctca cggattcttc ttaggggttc gagaaagctc attcgatatt
     1021 cgctccctca ggatcatcat tcaacttcaa accgcgtaag gcttcatcca tgctagtgac
     1081 ggcatcccgt cgcattcctg cagcgggcgt accgcaatat gggcagatgt tccagggcag
     1141 ctccatgagt ctgttgcagt tctcacagct cttcttgagc ttggtatggc aattggggca
     1201 gacctgccag tcgtctttga ctcggcgctc acagcccggg caaagcggca aatcctccag
     1261 agcttgaagc agggcttctt cctcgagcgt tttttggtat tcttcttcca atgtacgcgg
     1321 tggacgaagg atcaggtaca caagcacacc aggtaaattg agaacggcga caagcagggt
     1381 agccagggtt tgcaccaacg gatcacgcgc tcgagtgcgt atatcgcgat aggtccaaat
     1441 gacaagtgag atccaaagcg cggcaaggaa ggctccgccg aagccggtta gaacaagaat
     1501 gagatttcca ataaatgttg ggtcaaatgt catgcaacct catcgattga caattgtatc
     1561 tcatctcatg gcacatcgtg atgaaagtgc cattcgccat cgccttgatc cacaaaagac
     1621 cgcgccccca atacctcata aaatgtaaca cccggtcgca gataaattgg cgcaccattg
     1681 actccggcca gcaggagcgg ctgatccgga ttcaggcggt accaccgagc caccaggccc
     1741 actccatcgc ggaagacgta agcctccccc gagcccgtca agtcaatttt aaacacttca
     1801 tcatcttcat caaatgaatt cgtaaaggta tgcgatgcaa gcaggtaaac aatgttcgaa
     1861 gccgtgaccg gccgctcggt gaccgcatct accatcacat catatttttc aggtttgttg
     1921 tctcgtgtat cggccacctc ctgataacgg acgtatgcct gagtctctgg ttgataaacc
     1981 cagtagtgat acgaatcgac ggaatagtcg gtgaacattt gtgtcccggc aagatctgct
     2041 tgcgggactg tatcgctgaa caatccactg cggaattgag gacgctgatt atccaaccca
     2101 ttctcaagga tacaatcctc ccacaacagc gtattgaaat aggagttgtt atagacttcg
     2161 atatttcgac tttccatcaa acgaaaggga gggcatgatc cagtaccggg caccactaaa
     2221 aaatcgtaaa aatcactgag taaaaagtga ttcatctcgc gtgggtcagc gaatttgaac
     2281 accagataag cctgatacat gcgctgaatg tgttcgtcaa aatatcgacc ggagcggaca
     2341 ggccctaccc actcggaatt gtttccgtag aagacggcaa taaagcgcgt cagcccgtct
     2401 tctatgtaat attcatagat attatcagcc agggtcaagc ctgattgggg ccgaatataa
     2461 cgcggataat ttgccacctt gatcgccatc gggcgacggt taagtaagtt caaatcagaa
     2521 ggttccaaac cagtaagcgg attaaggttt gggggtgaaa tgagcgtggg aaagtcggca
     2581 ggcaaagacc ctgacgcagc tacaacttcc gttggtccat ttggcaaagg agtgagcgtg
     2641 gggagattca gaggagtggg tgcagcggca gaatagggag agtcggagag attttccggt
     2701 tgaggctgaa agggtgtgga ggtggggatc ccgctgtttg gaacgtcatc gacaccaatg
     2761 tcacaagaag aaagcagtac aaccaggcac aatactaaat tgaggctgaa aaaacgcatt
     2821 gtttgattct acaacaaaca caatcctctg ccaaaccctg cttcggtact ctctgcgccg
     2881 gtgaaagtgg acaggtcaaa cctgataaaa taaccttaca tgctcatttt tattacaatc
     2941 ttagtctttt tcctcaccgc cttaacactt ctcattttga gattctttgc acctcaattt
     3001 cgctacagct gggtgattgc aaccgtcggt gcgcttgctg gctggatcag cgtttttgtc
     3061 tggcaagtgc aaatgcccat caccttgcag ttctccatct ggcagccaag cacgctcttt
     3121 ttacaatctc cggcgttgat cgcggatggc atctcgtggg cgtttgcagt cagtctggca
     3181 gcactctgcc tggcgataat cattacatcg gttgtgcgtg ccaattttcc cagtccactt
     3241 acgtgggtag gcagcctggt tcttacgtcc tttggaatac tggctgtggt tgcggataac
     3301 cctctcacgc tcgtcttgat atgggcagct atagatctgg tggagctcat cacacaaatg
     3361 cgctttgtag acaacccaag attgagcgag cgtgtggtca ttgcattcgc atcgcgggca
     3421 gtgggaattt tgactctgct gtgggcggat atggtaagtg cagctagtgg tcaggtcctt
     3481 ggttttcgcc ttgcctcgga ggcaagccca caggcaggtc tttttctcgt gatcgcggcg
     3541 gggcttcgca ttggcgtttt gccgcttcat ttgccttatg caagtgaatc ctccatccga
     3601 cgcgggtttg ggacaggttt gaggatggtc tcagcggcct ccagcctgat tctgttggcg
     3661 cggattccat ccagcagtgt gacatcgccc tttaccccct atttgttgat cctggtttcg
     3721 ttagcagctg tttacggaag ctggatgtgg ttgcgggcgc cagatgaact taccggcaga
     3781 ccctattggt taatcggtat gggctctctt gttatcgccg ctggactgcg tgctaatccg
     3841 gttggcgcgg cggcctgggg gtgtggactt atcctggccg gcggtgcgct gttcctgtct
     3901 tcagaacaga acaggtggct gactggtgcg ttgcttatcg gggctttggg aatttcatcg
     3961 cttcccttta cgctgactgc aacaggttgg aatagcggag ggccaagttt ctggtttaca
     4021 tggcctttcc tgctggtttc acaaggcatg ttgatcgcag gttacattcg tcacagccga
     4081 agaacagtca tgcgcgagag caatgaagac caacccatct gggcaaagaa tgtataccct
     4141 tttggaatct atcttttggt attgatggtc atcctgcttg ggttatatgg atgggatggc
     4201 acactgcaat ttggcaattg gctcatgggc ctgatagcat ccgtgctgac cattggtttg
     4261 ctctggctta tgccgcgttt gcgaatcttg aatcctgttc gcgcacactg ggttcgaccg
     4321 gtcaatacat cctggatgga atggagttat caaattcttt ggggactcta ccggcagctt
     4381 ggaaaattga gcaatgcctt cacaaatatg cttgaaggtg aaagtggcat tatgtggacg
     4441 ttgctttttc ttgttctttt tatttcattc ttcaggcaag gatcacccta agctatggcc
     4501 gagatcttgc cctggatcgc ggttgtcgtc attcttatca catcggtagg cttactatta
     4561 gtgcgagact ggcgtttaag catcatcttt ctggcagttc aatatttgtg tatgcttatc
     4621 atggtccttc aacactggcc cctgggaatg gctactgtga aagtggtagc gggttggatg
     4681 tccgctgcta ttctcggcat gactcgttcc gggttggcct cacagggatt cgatgaagaa
     4741 agcatttggc cccgcggcag actctttcgt ctcttcgcgg ctgggatggt tggtgtgatt
     4801 gtctcagcgg ttacgccgag tgtctctaca atcatggcag acgccggttt tgccgtcacg
     4861 agtggaagct tacttttaat aggtatggga cttcttcacc tggggatcac cacatcgatc
     4921 ctgcgcgtca ccatcgggct gatgacagtc ctctccggat ttgaaatcat ctactccgct
     4981 gtggaagggt ctgtgctcgt tgccgcgcta ctggcgatta tcaatttggg tctggcgctt
     5041 accggctcct atcttatgat cgcttcctct tcctcggaat cagaaatcga gtcggtatga
     5101 atgctccgac gatttggatc gtcgtcccac tttttttcgg tatcttgttg ttgttcatca
     5161 acaatcaacg agcccttagc atcctgggag gcgtgttttc tgcctcgctt gcactgatcg
     5221 cccaattcgt accgattgaa caagccacgc tcgttggagc agcctcgttc aaaatcgact
     5281 catccctcac tgttttaggg cggacgcttg taatccttcc cgcggagggg tcgttgctgg
     5341 cattgatcta cggggcaacc gcattatggt tttttggtgc ggaagtttcc aagacagcca
     5401 cccgcatcgt acccatcgga ttgatgatca ctgcgctttt agtcggctcg ctggcggtag
     5461 agccatttct atttgctgct cttttcatcg aaatggcgat acttctcgcc gttccccttc
     5521 tgaccagtat ctattcaccc cccggcagag gcgtggtacg tttccttatt taccaaacac
     5581 ttgccatgcc tttcatcctg ctcactggct ggcttctggc aggcgtagag gctagccccg
     5641 gcgacctggc ccttgcagct caatcctcgt ctatgcttgg gctgggattc gcatttctgc
     5701 ttgcgatctt cccactttac aattgggtgc ccatgttgat ggaggaaaca tccccataca
     5761 ccgctgggtt tctgctgtgg attctgccaa ccatcacgat tattttcggt gccggttttc
     5821 tggatcgcta ttcctggctc cgttcgtcga ccgaactcat caatgcactt caatttacgg
     5881 gactcttgat gatcgttacc ggtggcttgt tcgccgggtt tcagcgacat ctcgggcgga
     5941 tcatggcgta cggcgtcatt gcagagactg gtttttcgct cctggctctt agcctcgatc
     6001 cgaggttagg cataccaatt ttgtttttgt tgatcccttc ccgcgccctt gggcaagctg
     6061 tatgggcgct ttccctgacc gtgatcaaag atcaggtaga aacaatgcga ttccgctcat
     6121 ctcgcggact gctgcgtgtt actccgattg caggtgccgg gatgatcgtc gccgcccttt
     6181 caaccggggc atttcctctg ctggcaggtt tcccttccag actggcgttg tgggaagggc
     6241 tttcgcgggt atcgctgggc gcatcggtgt ggatgggaat tggcattctt gggctgctga
     6301 ccagcgcctt tcggtctctt gcagtaatca gtatggctga agaatacaca agctgggagc
     6361 ggcgtgaaag cctcgcacag gcgatcatgc ttggggtggg aatgatcgga cttttcatcc
     6421 tgggcttatt cccccaggtc atccaatatt ttttatccgg attgcctgcg atgttcgaga
     6481 atttaggtcg ctgaagttgt taaaacagca cgaggtataa tccactgcaa gtccctcgga
     6541 ggaagctatg gaatatgaac aactcgtcaa acgccttgaa tgggtggaag gtgaacaccg
     6601 aaaagccaag tcaacgatcc ttacgctgga agaacgcata gcggcactcg acagcaatat
     6661 cgaagtggtt gcgaaacaga tcaaaccgct tgataagcgc attgcggatg ttgccagcac
     6721 agcatcccgc ctggaccagt ttgacgccat cttcgccaaa cagcgtgaag atatgaataa
     6781 aggtctggat gatatagaga aaagacgcca aaaacaggac aatgaaattg cgaaacgcca
     6841 ccagcaggat ttggagcctc tggtcaaagc attaagcgat ctacgaaaat ctatggaaag
     6901 cgaatttcct cccattaagc gtgatatcaa ggcacgcatc attgaggaga ctcgcctaca
     6961 aaaggaaatt aaggaacttc gacctccgat tgatgaagcg gtccgcattg cacatgaggc
     7021 aacgctcaat caaaaggttt ttgaggaagc ccgcaaacag gaagccaagc gggtcacaga
     7081 tttgcagggc gaactaaccg ccattcgggt gcgtattgat gaggcgcgcg ccaaagccga
     7141 cctgaatgct gatggttgga ggaatatgga caaccgcatc aaggaattga tggcttctga
     7201 aaccgaa
//
LOCUS       DASTJP010000068         7402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_286986, whole genome shotgun sequence.
ACCESSION   DASTJP010000068 DASTJP010000000
VERSION     DASTJP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286986"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFQ23_02050"
     CDS             complement(<1..203)
                     /locus_tag="VFQ23_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9905384.1"
                     /translation="MLSNPKEQEKLDPRFKRTRNLILKSFGELLAEKNFENISVQDVT
                     DKAEINRATFYKHFVDKYALLDYS"
     gene            340..711
                     /locus_tag="VFQ23_02055"
     CDS             340..711
                     /locus_tag="VFQ23_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9905385.1"
                     /translation="MNIALWIAQGLLAVMFGMSGVMKTFQTAKTKEQMSWAKNRSDGF
                     VRFVGISEVLGAIGLILPLVTGILTWLTPLAAIGLALIQLLAIFTEHLPKKEFNVIPV
                     NILLIALALFVVIGRWSLIAL"
     gene            743..1387
                     /locus_tag="VFQ23_02060"
     CDS             743..1387
                     /locus_tag="VFQ23_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9905386.1"
                     /translation="MNVTIIGAGNMGRGIGTRAVAGGHSVTFVDANPETAEKAAADVK
                     ASAKNGVKVSTASLGEAELGDVVVFAVWYGTNIELAKQLGNRLAGKVVVDIANPLNST
                     YDGLATAPDSSSAEDLAKAIANGAKVVKAFNTTYAGTLLAGEVAGQPLDVFIASDDAD
                     AKAKVAQIVTDGGMRPVDTGPLSRARQIEGMQFLHIVTQGSLGTNWASSIKILG"
     gene            1444..2094
                     /locus_tag="VFQ23_02065"
     CDS             1444..2094
                     /locus_tag="VFQ23_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET9905387.1"
                     /translation="MLTISETKLELEPNTEFSVLATDEQIERTVSALEANGIQTVVAE
                     NGEDAKRIFFELLPEGSEVFLGASVTLETLGIKDEIDSSGRFNALRPKMRAMDRATQG
                     KEIRKLGGAPDYAAGSVHAVTEDGHVLIASNTGSQLGPYASGAGKVIWVVGAQKIVKD
                     VNEGLRRIEEYCYPLEEVHMQDLYNMSTSVNKVLILNREIRPDRITMIIVKEELGY"
     gene            2181..3062
                     /locus_tag="VFQ23_02070"
     CDS             2181..3062
                     /locus_tag="VFQ23_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9905388.1"
                     /translation="MANDRNPIKFGYFLIPNADAPLLSLAQEAERLGLDYIGIQDHPY
                     QRRFVDTFTLLSMIAATTSRIRLFPDVANLPLRPPAVLAKAAASIDILSEGRFELGLG
                     AGGFWDAIEAYGGSKRSPGEALEAFAEAIEVIRTVWSGERNLRFDGQHYHLRGAHSGP
                     VPAHPIGIWLGVTGPRALKLTGQVADGWVPSFRGDLLKLVDMTNQINDAAGEAGRDPA
                     SIRRILNVNGVITDGQSSGILQGPVDQWADELSNLAITYGFDTFIFWGEGDGQLQKFA
                     EEVVPAVRAQVALERTG"
     gene            3137..4057
                     /locus_tag="VFQ23_02075"
     CDS             3137..4057
                     /locus_tag="VFQ23_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9905389.1"
                     /translation="MDQTQTQEKTEFSIHPRTMMGHISLTVANLENQIEFYTKAMGFK
                     LHWRDGTKAGLGAGGPDLLLLTEEPDRKKYNRVTGLYHVAYLFPNRRELAIAMARLFA
                     IKYPNSPTDHIMTKTTYLDDLEGNGIELYCESPEDGAWTFKDGKFESYRTDGTWSDGR
                     EALDVEALFKHIREEDRLDAPVPTDLKIGHVHLHVRDVDEAVDFYHGIIGFDIMGVSK
                     PFQMGFVSAGGYHHHLGLNTWQGRGAPPPPQDAVGLRYYTIDLPTQAAYDEVVARVDA
                     AGIPSNQTETGLLLHDPSQNGLILRVASIS"
     gene            4245..4910
                     /locus_tag="VFQ23_02080"
     CDS             4245..4910
                     /locus_tag="VFQ23_02080"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9905390.1"
                     /translation="MLPIGDDNSARRITPLVTYALIAINVLVFLLELSSGDAFIERWA
                     FVPTRFLANPIGELPTLLTSMFMHAGWLHLLSNMLYLWIFGDNVEDRFGHGLFTVFYI
                     LCGLGATFAQLAVSMGSSIPNLGASGAIAGVLGAYIVMFPGSGVNVLLGRVVTRMSAL
                     IVIGFWIILQIFSGIGTFTASSQAEGGVAYMAHIGGFFAGVLLTFLLGGNRRALPATT
                     YRR"
     gene            4965..5396
                     /locus_tag="VFQ23_02085"
     CDS             4965..5396
                     /locus_tag="VFQ23_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9905391.1"
                     /translation="MKKCSDIMTKDLIFSHPTDTVDDVAKLMKKEDIGPVLIVEKGDQ
                     GKRLVGIVTDRDLALKVVGEGRDPKKTTVKDVMTTKVVTCRADDDVQNAMNAMAQHQL
                     RRIPVVDEYGMLVGIISQADVATRVDEPEQTAEVVREISQD"
     gene            5517..6344
                     /locus_tag="VFQ23_02090"
     CDS             5517..6344
                     /locus_tag="VFQ23_02090"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9905392.1"
                     /translation="MDQQKDLLSIGAFASMTRLSIKALRLYDQLDLLQPRHVDPQTGY
                     RFYGIDQLSSARMIRNMREMDMPLATIRQVLAALTSSPAQAELLVREYAEMREGQVRQ
                     IRVQVGQFIQQIQQEQNPMSLEVNVRQLAPQQVLSVTHHIKVSKLDDTIRKSLDTMYG
                     LLKEQKVEAESAPFGIFHGAINEQEDGPIEICLPVSGQILGKGDVQVKELQGGDAACV
                     TMLGAQTDFPAILGAYDAAADWIQKNGYQMAEPPREIWHSGPGGADEPKMEIVWLFK"
     gene            6414..6890
                     /locus_tag="VFQ23_02095"
     CDS             6414..6890
                     /locus_tag="VFQ23_02095"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HET9905393.1"
                     /translation="MPTIGKPRIDKRPKQVYMGIRTIAPFKGMFKVVSKIAGELNAWV
                     EENKIKTAGPPFLRYHVIDMRGFMDISYCVPVRKALPDDGNVKAEVLPAGRYASLIYS
                     GGGISGNRALIEWVRAQGMDFDRWDSEQGDNFRSRYETYLTDPTVEPRKSKWQINN"
     gene            6963..>7402
                     /locus_tag="VFQ23_02100"
     CDS             6963..>7402
                     /locus_tag="VFQ23_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9905394.1"
                     /translation="MSKKLHILLVFTLILNACAVTTPEPTSTPAPTPIPSPTPAAWEL
                     EGWNIVWHDEFDGPELDRNNWTFDIGGNGWGNSEWEAYTDRPENVRIENGMLVIEARE
                     DPTLPAGRPYSSARIKTQGLHAWQYGRIEARVKLPYGQGIWPAF"
BASE COUNT         1908 a         1740 c         1985 g         1769 t
ORIGIN      
        1 gagtaatcga gcagagcata tttatccacg aaatgtttat aaaaggtggc gcggttaatc
       61 tctgccttat cagtcacatc ctgcacagaa atattctcga aatttttttc agcgagcagt
      121 tcgccgaagg atttcagaat caggtttcgg gtccgtttga agcggggatc cagtttttct
      181 tgttctttcg ggttggacaa cattttggct ccagtgttgc ttagtcaaca aaatccagtg
      241 tttggtggtt gacctgaaaa ggtaaggcag gtaaattata caacatctgt tgtttaagca
      301 tacaactgta tattatcact actcacaagg agagtcaagt tgaatattgc attatggatc
      361 gctcagggct tgctggcagt catgtttgga atgtctggtg tgatgaaaac cttccagact
      421 gccaaaacga aagaacaaat gtcgtgggca aaaaatcgtt cggatggttt cgttcgcttt
      481 gtagggatct cggaagtgct cggcgccatc ggcctaattc ttcccctcgt aacgggaatc
      541 ctgacctggc tcacgccgtt ggcagctatt ggacttgcat tgattcagtt gctggcaatc
      601 tttacggagc accttcccaa aaaggaattt aatgtcattc ccgtgaatat cttattgatt
      661 gcactggctc tgttcgttgt cattggacgc tggtcactga tcgcactcta aacaaatcat
      721 aaacttatta aggagattag aaatgaacgt aacaattatt ggagcaggaa acatggggcg
      781 cggcattggc acccgcgcag tggccggtgg tcactcggtg acttttgtgg acgcaaaccc
      841 tgaaacggct gagaaagctg ccgcggatgt aaaagcatct gcaaagaacg gtgtaaaggt
      901 ttctaccgcc agcctcggcg aggctgagct tggcgatgtg gtcgtgtttg cggtatggta
      961 tggcaccaac attgaacttg cgaaacaact gggcaataga ctcgcaggca aagttgtggt
     1021 ggatatcgcc aatccattga attcaaccta tgatggtctt gccacggccc ccgattcatc
     1081 ctccgccgaa gatctggcaa aggcgattgc caacggggcg aaagtggtca aggcgttcaa
     1141 caccacctat gcgggcacac ttcttgcagg cgaggtcgcg ggccagcccc ttgacgtttt
     1201 tattgccagt gacgatgcag atgccaaagc caaagtcgca cagatcgtta ccgatggtgg
     1261 catgcgcccg gtagataccg gtccgttgag ccgcgctcgc cagatcgaag gaatgcagtt
     1321 cctgcacatc gtcacacagg gcagtctggg cacgaattgg gcaagctcga tcaaaatctt
     1381 agggtaagcc catattgata acttcaatca atcgttaagt agattaatag gaaagatata
     1441 cgcatgttaa ctatatcgga aacaaaatta gaattagaac cgaatacgga atttagtgtc
     1501 ctggcaacag acgaacagat cgaacgtacc gtcagcgccc tcgaagccaa tggcattcag
     1561 actgttgttg ctgaaaacgg cgaagatgca aaacgcatct tctttgaact actgcctgaa
     1621 ggctcagaag tatttctggg tgcttcggtc acgcttgaaa cactggggat taaggatgaa
     1681 attgattcgt ccggacgttt caatgccctg cgccccaaaa tgcgcgcaat ggaccgtgcc
     1741 acgcaaggca aagagattcg caaactcggc ggggcacctg attatgctgc agggagcgtc
     1801 catgcagtga cagaggatgg tcacgtgttg atcgcatcga acactgggag tcaactgggt
     1861 ccctacgcct ccggcgcggg gaaagtcatc tgggtagttg gcgcgcagaa gatcgtcaaa
     1921 gatgtgaatg aaggattgag aaggatcgaa gaatattgct atccgctgga agaagtccat
     1981 atgcaggacc tttacaacat gagcaccagc gtgaataaag tgttaatttt gaatcgcgag
     2041 atccgcccgg accggattac aatgatcatt gttaaggaag aattgggcta ttagtcgtag
     2101 cacaacatga tcgctgccat ggcattgatg aatgctgctt ggcacgaatt cgaagataaa
     2161 gatctgcaag gaggcatcgc atggctaacg atcgcaaccc gatcaaattt ggatattttc
     2221 tgattccgaa tgcagatgcc cctttgcttt ctcttgcaca ggaagccgag cgccttgggc
     2281 tggactatat tggaattcaa gaccaccctt atcagcgacg gtttgttgat acgtttactt
     2341 tgcttagcat gatcgccgcc acaacatcac ggatcagact tttccctgat gtggcgaatc
     2401 tgccgctgcg acctccagcc gtattggcaa aagctgcggc gagtattgat atcttaagtg
     2461 aaggtcgctt tgaactgggg ctgggcgccg gagggttttg ggatgcaatt gaagcgtatg
     2521 gaggctcaaa gcgtagcccc ggtgaggcgc ttgaggcttt cgcagaggcg atcgaagtca
     2581 tacgaacggt ctggagtggt gaacgcaatc tccgctttga tgggcaacac taccatttac
     2641 gcggcgcgca ttcaggccca gttccggccc atccgattgg gatctggctc ggcgtgaccg
     2701 gcccgagagc ccttaagttg accggtcaag tggccgatgg ctgggtaccc tccttccgtg
     2761 gcgatctttt aaagcttgta gacatgacca atcaaataaa tgatgccgcc ggtgaagcag
     2821 gacgtgaccc agccagcatc cgtcgaattc tcaacgtgaa cggggtaatt acagacgggc
     2881 agtcgagtgg tatattgcag ggaccggtgg atcaatgggc agacgagcta tccaatcttg
     2941 ccatcaccta tggttttgat acctttattt tctggggaga gggagatggc caattgcaga
     3001 aattcgctga agaagtggtg cccgccgtcc gggcgcaggt tgccctggaa cgaaccgggt
     3061 agcctgaaga ggaattagaa ttctttggaa agcaaagtga tgctgatgtt gtctaattcc
     3121 cagaaagaga agagaaatgg atcaaacaca aacacaagaa aagacagagt tttccatcca
     3181 cccaagaaca atgatggggc atatttcgct cacggtggca aaccttgaga accagatcga
     3241 gttctataca aaggcaatgg gcttcaaact ccattggcgc gatggaacga aggccggtct
     3301 cggggcaggc gggcctgact tactcctgct caccgaggag cctgatcgaa agaaatataa
     3361 ccgggtgaca ggtttatatc atgtagccta tctgtttccc aatcgtcgcg aactagcgat
     3421 tgcgatggcg cgtttgtttg cgatcaaata tcccaactcg ccgaccgatc atattatgac
     3481 caagaccacg tacctggatg atctcgaagg caacggtatt gagttgtatt gcgaatctcc
     3541 tgaggatggc gcatggacgt tcaaagatgg aaaatttgag tcatatcgca cggacggcac
     3601 atggagcgat gggcgcgaag cactggatgt cgaagccttg ttcaagcaca tccgggaaga
     3661 agatcgcctg gacgcgcctg ttccaactga tttgaaaatt ggtcacgtgc atttgcacgt
     3721 ccgtgatgtg gatgaggcag tggattttta tcacggcatc atcggctttg acattatggg
     3781 tgtctccaaa ccctttcaga tgggattcgt ctcggcaggc ggatatcatc atcaccttgg
     3841 gctcaacacc tggcagggac gcggtgctcc accacctcca caagatgctg taggtttgcg
     3901 ttactacacc attgacctgc cgactcaagc ggcgtacgat gaagtggtgg cgcgggtcga
     3961 cgcggcaggt atcccgtcga atcaaactga gacggggctc cttttgcatg atccatcaca
     4021 aaatggcctg atcctgcggg ttgccagtat cagttaaata ttttcgttga agtagaacct
     4081 ccaaagcgag aatgctttgg aggtttttta tttatagtag gggattccct gctatgggcg
     4141 tgggcaagtc cgtattgacg gtatgtctgt atatctacat aatgatgacc agcacaaatc
     4201 attaggggtt caatttcatt tcgaatcaac aaaggagaat acgtatgtta cccatcggag
     4261 atgataatag tgcccgcaga attacccccc ttgtaaccta tgcgttgatt gcaatcaatg
     4321 tgttggtttt tcttttggaa ctgagtagcg gagatgcctt catagaacgc tgggctttcg
     4381 ttccaacccg cttcctggca aaccccatcg gggaattgcc cacactgctc acatccatgt
     4441 ttatgcatgc gggatggctg cacctcttaa gcaacatgct ctacctgtgg attttcggcg
     4501 ataacgtaga agaccgcttc ggtcatggac tgttcacagt attctatatt ttatgtggac
     4561 tcggcgcaac ctttgctcaa ttagccgtca gcatgggctc aagcataccg aacctgggag
     4621 cctcaggagc cattgccggt gtcttgggtg cttatatagt gatgttcccg ggcagcggtg
     4681 taaacgtcct gctgggacgt gtggtcacgc gaatgtctgc tctgattgtg atcgggtttt
     4741 ggatcatatt acagatcttc agcggcatcg gtacatttac tgcttcctca caggcagaag
     4801 gtggtgtagc atatatggca catattggcg ggttctttgc aggagttctt ctaaccttcc
     4861 tgcttggtgg aaaccgcaga gccttacctg ccaccaccta tcgacggtaa atcagacgga
     4921 aaataatagt ttcaacacat cagacttgaa caaggagatt aataatgaaa aaatgcagcg
     4981 atatcatgac aaaagacctg atctttagcc atcccaccga tacagtggat gacgtggcta
     5041 agctaatgaa gaaagaagat atcggccctg tcctaattgt ggagaaaggt gatcaaggga
     5101 aacgattggt cggcatcgtt accgaccgtg acctcgcgct caaggtggtt ggcgaagggc
     5161 gcgatcctaa aaaaacaaca gtgaaagatg tgatgacaac gaaagtcgtc acctgccgtg
     5221 cagatgatga tgtgcaaaat gccatgaatg caatggcgca acaccaattg cggcgcattc
     5281 ccgttgtaga cgaatacggt atgctcgtgg gcatcatttc acaggctgac gtggcaactc
     5341 gcgtggatga accggaacag accgcagagg tggtccggga aatctcacaa gactgaccgg
     5401 cgcttcccgt caacaaaacc gtgagacact cgaaggcttt accgcctcga gtgtcttttt
     5461 ttgttcttcc tctctaccct tgactctacc ccttggtgag attgtatact gcggatatgg
     5521 atcaacaaaa agacctgctt tccatcggcg cttttgcgag catgacccgc ctttcgatta
     5581 aggcgctgcg gctctacgat caattggacc tcttacagcc gcgtcatgta gacccccaaa
     5641 cgggctatcg cttctatggc attgaccagc tttcaagcgc ccgcatgatc cggaacatgc
     5701 gcgagatgga catgccgctt gccaccattc ggcaggtgct cgcggcattg acgtcctcgc
     5761 cagcgcaagc cgaactgctg gtgcgggaat acgcagagat gcgtgaaggg caggtcaggc
     5821 agatccgtgt acaggtcgga caattcattc aacaaatcca acaggagcaa aaccccatga
     5881 gtctggaagt aaatgtaagg cagcttgcgc cgcaacaagt gctgagtgtc acccatcata
     5941 ttaaagtgag caaactggac gacaccattc gcaaatcctt ggatacgatg tacggtctgc
     6001 tcaaagagca aaaggtggag gcggaaagtg ccccgtttgg gatctttcat ggcgccatca
     6061 acgaacagga agatggtccg attgagatct gtctgcctgt gagtggacaa atcctcggca
     6121 aaggcgatgt gcaggtcaaa gagttacagg gaggtgatgc tgcctgcgtc acaatgcttg
     6181 gtgcacagac cgatttcccg gccatcttgg gggcgtatga cgccgcggca gactggatcc
     6241 agaagaacgg ttatcaaatg gcagaaccgc cccgcgagat ctggcacagc gggccaggtg
     6301 gtgcggacga gccgaagatg gaaatcgtct ggctgttcaa ataaatcaca gaccgtgtgc
     6361 ctggttaggg cacacggtga atttaagcgt cacaaggata gcttaggagg agcatgccaa
     6421 ccatcggtaa accgaggata gacaaacgtc caaaacaagt ctacatggga attcgaacga
     6481 tcgcgccgtt caaagggatg ttcaaagtgg tcagcaaaat tgcgggtgag ttgaatgcct
     6541 gggtggagga aaacaagata aaaacagcgg gtccgccatt tttgcgctat cacgtcatcg
     6601 atatgcgcgg gttcatggat atctcgtact gtgttccggt tcgaaaggca ttgccagatg
     6661 atggaaatgt gaaagccgag gtgcttccgg caggccgcta cgcgagcttg atctattcag
     6721 ggggtggcat ttcaggaaac agggctctca tcgaatgggt ccgcgcgcag ggcatggact
     6781 ttgaccgctg ggatagcgaa cagggcgata acttccgctc gcgctatgaa acctatctca
     6841 ctgatccgac agtcgagcct cgcaagtcaa agtggcagat aaataattga tggtgtacat
     6901 gcatattcat ggtaaattag gtgggattaa ttctgctaaa gaatccacgt caaaaggttc
     6961 atatgtccaa aaaacttcat attcttctag tctttactct tattttgaat gcgtgcgcag
     7021 tgacaactcc tgaacccact tcaacacccg ctcccactcc aatcccatcg cctacccctg
     7081 ctgcgtggga gctcgaggga tggaatatcg tgtggcatga tgaatttgat ggacctgaat
     7141 tggatcgaaa taactggacc tttgacattg gtggcaatgg ctggggcaat tcggaatggg
     7201 aagcgtatac cgatcgacct gaaaacgtac ggattgaaaa tggaatgctc gtcattgaag
     7261 cacgcgaaga ccccacgtta cctgccgggc gtccttactc ctcggcacgc attaagactc
     7321 aggggcttca cgcctggcaa tatggacgta ttgaagcgcg cgtgaaactc ccgtatggtc
     7381 aaggaatctg gcctgccttc tg
//
LOCUS       DASTJP010000069         6598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1199830, whole genome shotgun sequence.
ACCESSION   DASTJP010000069 DASTJP010000000
VERSION     DASTJP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199830"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2371)
                     /locus_tag="VFQ23_02105"
     CDS             complement(<1..2371)
                     /locus_tag="VFQ23_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9905395.1"
                     /translation="MAKLFVSYSRKDSVAARKLIEAFKSVVQQEVWVDWESIPPAVDW
                     LEQIFRGIEESDAFLFLISPDSVASEVCNVEIGRAAQNNKRIIPIVLRDVDPKITHEN
                     IRKLNWTYMREGDDFEEGLQKVKTAIELDLDWLEEHRRLLVRSLEWHRKKDPSLFLRG
                     RDLRNAQHMVATATAKDPIPTDLQRTFIQHSQRSERNRITAWIAAGTALLIMAILSYW
                     ALVQSDLAKENAIVANQQREIAEKNAVQALENAREARRAQLEAEDARSVAEEEREKAV
                     QQEQIAASQRSAARAQIYQTRTGELYTSTLLAIDSWKRSPSDEAEEILRENISLLPLP
                     IVQASQEGNINALELNQNEGIFLTAGDDGTACVWNLNDMKQRFCETSASVMNDAVFAL
                     GGKSLVTGDENGVVRVLNAENGNLEREFASGSPVRDVDIGPDGKLLAISRANGLITIV
                     DILNQTNNGYNLTLSGRLTVADFSPNGRWMAAGSESGTVTVWNLSTRQIYSSGRHKGE
                     VLTIRFSPDSRFVVSGGADNYAVGFDTQRGEEVFRLLHADWVQDVAFPQTGTWLATAS
                     DDARVRIWDINTGKERLIMFQDGAVTNVKISSDGKWIAGTGEDRTVRVWSVYTGVEML
                     QIPLPAPGALLDFSNDHQSLISTDNTGNISQWDISGMVAPLNYIQFDKLIWISEFAPA
                     GNTLLASDANRVWVLDSNDLSVPGTRPSASSALEYGSNIYDLVISPDSAWIGTSTYDN
                     EYFIRNLRTQVATRVYPSTDANALAFSADSSRFFTGTIEGGLEIWDTKTA"
     gene            2446..3237
                     /locus_tag="VFQ23_02110"
     CDS             2446..3237
                     /locus_tag="VFQ23_02110"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9905396.1"
                     /translation="MLNDSNQFSREAWDANAEHWDSRMGDEGNDFFNLLCWPVLASFL
                     DVQPGQRILDIACGNGLTSRRLAALGIRVTAFDFSANLIELAKARPNPESRIDYHVID
                     ATDEQALLKLRDQQFDSALSNMALFDMPDIEPLFRVLPKLLKPNGVFAFSLMHPAFNN
                     PSATLLAEEWDDGQIQTRYAVKVPRYINQFQSQGLALRNQPKPQLYFHRPIRYYMNIA
                     FQNGFVLDGFEERAFPPDHPQTATLGWGGKFSEIPPALIARMRVR"
     gene            complement(3286..4245)
                     /locus_tag="VFQ23_02115"
     CDS             complement(3286..4245)
                     /locus_tag="VFQ23_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HET9905397.1"
                     /translation="MEPIKGLHHVTAVARDPQRNIDFYRNVLGQRFVKRTVNFDSPDT
                     YHFYFGDEAGSPGSILTFFAWPNMRRGIRGNGETNALAYNVPAGSLGFWQEHLKQNGI
                     TPKAIEKRFGEDVLAFDDPDSMRVELVETASAAPVRFWEAGPIPQSYALQGFHSVTLW
                     LDEVEPTADLLTKNMGYRAAGNESDRYRFVGDSNVSGPIVDIVHRPGKTPAGFGAGSI
                     HHIAFRVPTDEKQLDYQNAIRQAGFRVTEVMDRSYFHSIYFRELGGVLFEIATDTPGF
                     ALDEPVESLGESLKLPEWFEPNRVAIEQSLPPVTLKPFEKVAS"
     gene            complement(4377..5207)
                     /gene="speE"
                     /locus_tag="VFQ23_02120"
     CDS             complement(4377..5207)
                     /gene="speE"
                     /locus_tag="VFQ23_02120"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET9905398.1"
                     /translation="MSIWFTEELHPYYRKGIRIKRVLADEQTQYQHLQFVETEFFGNA
                     MILDGIIQMTERDNMGYHEMIVHVPMLAVGQPKRVLIVGGGDGGSLQQVLRYPSVGEV
                     VVCELDQRVVDLSREHFTAAFGDPWMDPRAKLLVRDAFGFLEENPGQYDVIISDTTDP
                     IGMAERLFSNEFYQLIVRALVPGGAAATQCEQPFFDSELIKEIYKSAKELTKNPAYYY
                     ANIPTYPGGGIGFMYVSDTPWTNGLKSPYPPGENKYLNPEIHQAAFALPEFFRKELYG
                     "
     gene            complement(5277..5663)
                     /gene="speD"
                     /locus_tag="VFQ23_02125"
     CDS             complement(5277..5663)
                     /gene="speD"
                     /locus_tag="VFQ23_02125"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HET9905399.1"
                     /translation="MNATLKLGEHYIFDLSDCNPEILMDSERSYSLFAQAVRESGLTV
                     VDEGYYRFSPHGFTCFLLLAESHASLHAWPEYGYCAIDLFTCAIGKDMMPLLMRLKEA
                     FGADDFALRKLDREAAIEPQSMPIAV"
     gene            5854..>6598
                     /locus_tag="VFQ23_02130"
     CDS             5854..>6598
                     /locus_tag="VFQ23_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905400.1"
                     /translation="MIYLLIEFLDELVFGVGEIAWPLIRDDLHLTYTQIGLLLSMPGI
                     MAAFIEPFIGILGDVWKRRLLIIFGGLLFTLSLVMTSVSYSFIFLLSSLILFNPSSGA
                     FVNLSQANLMDSDPTRHEQNMARWTFAGSIGVLAGPLLLGLFVYVGLGWRGTYAFLAF
                     LSTLCLFAALRYLPPEKNSSLPFPSLRVIFDGFRAAFSALKRREVWRWLLLLEFADLI
                     MDVFLSYMALYFVDVGRVTQAQAGIAVTIW"
BASE COUNT         1559 a         1756 c         1589 g         1694 t
ORIGIN      
        1 tggcggtttt agtatcccag atttcaagtc caccttcgat cgtgccggtg aagaagcgag
       61 aactatccgc tgaaaaggcc agagcgttgg cgtctgttga tggatacact ctcgtcgcaa
      121 cctgagtcct caggttacga atgaaatatt cgttgtcata cgtggaggtg ccgatccagg
      181 cggaatcagg gctaatgaca agatcgtaaa tattgctccc atattcgagc gcagagctag
      241 ctgatgggcg cgttccagga accgaaagat catttgagtc caggacccaa acgcggttgg
      301 cgtcggatgc aagcagcgta ttccccgcgg gcgcaaactc ggaaatccag atcagtttat
      361 caaactggat gtaattcaaa ggcgcgacca tccctgagat atcccattga ctgatgtttc
      421 cggtattatc cgttgaaatc agagattgat ggtcattgct aaaatcaagt agggcgcccg
      481 gagcgggaag aggaatttgg agcatttcca cgccagtgta aacactccag acgcgcacgg
      541 ttcgatcctc acctgtgcca gcaatccatt ttccatccga actgattttc acgttggtga
      601 ctgcgccatc ctggaacatg atcaggcgtt ccttgccggt gttgatatcc caaatccgga
      661 ctctggcatc atcagaagca gttgccagcc aggtgcccgt ctgcggaaag gcgacatcct
      721 gaacccaatc cgcatgaagc aggcgaaaga cttcttcacc cctttgtgta tcaaagccaa
      781 ccgcataatt atctgctccg cctgaaacga cgaaccggct atctgggctg aatcgaatgg
      841 tcagcacttc tcctttatgg cgtccgctgg aataaatctg cctggtgctc aaattccaaa
      901 cggtgaccgt tccggattct gacccggcag ccatccaccg accatttggg ctaaaatccg
      961 caacggtcag cctgccagat aaagtgaggt tataaccatt gttcgtttga ttcagaatat
     1021 cgacaattgt gatcaacccg ttggcgcggg aaattgccag tagttttcca tcgggaccga
     1081 tatccacatc acggaccggc gaaccggatg caaactctct ttccaaattt ccattttccg
     1141 catttagcac gcgcacaaca ccattttcat caccggtgac aagggatttc ccgcccagtg
     1201 caaacacagc atcattcata acgctggcgc tggtttcaca aaacctctgt ttcatatcat
     1261 taagattcca tacacaagcg gtaccatcat cgccggcggt taagaagatg ccttcattct
     1321 ggttcagttc gagcgcattg atattgcctt cctgtgaagc ctgcacaatg gggagaggca
     1381 gcaaactaat attttcccga agaatttcct cggcttcatc cgatggtgac cgtttccagg
     1441 aatcaatggc aagcagtgta cttgtataca attctcccgt tctcgtttga tagatctgtg
     1501 cccgggcggc gctccgttgc gaggctgcaa tttgctcctg ttgaactgct ttttcacgct
     1561 cctcttctgc aacggaccgt gcatcttccg cttcgagttg agctcttcgg gcctctctgg
     1621 cattctccag cgcctgtacc gcgttctttt ccgcgatctc cctttgctga ttggcaacaa
     1681 ttgcattttc tttcgccagg tcagactgaa ctaatgccca gtacgaaagg atcgccatta
     1741 tcagcagagc agtgcccgca gcgatccagg ctgttattcg attcctctca ctgcgctgac
     1801 tatgctggat gaaggtcctc tgcaaatccg tggggattgg gtccttcgcg gttgccgtgg
     1861 cgaccatgtg ctgcgcattg cgtaagtctc tgcctcgcag aaataaactg ggatctttct
     1921 tgcgatgcca ctccagtgac cgcaccagta accgacggtg ttcctccaac cagtccagat
     1981 caagctcgat ggcggttttg accttttgta gaccttcctc aaaatcatcg ccctcgcgca
     2041 tataggtcca gttcagcttt cggatgttct catgcgtgat cttcgggtcc acatcgcgca
     2101 ggacgatcgg gatgatgcgt ttgttgtttt gcgcggctct gccgatctcc acgttgcaga
     2161 cctcagaagc gacggagtct gggctgatga gaaatagaaa ggcgtccgat tcttcgatgc
     2221 cgcggaagat ctgctccagc caatccaccg ccggagggat ggattcccaa tccacccaca
     2281 cttcctgttg aaccaccgac ttaaacgcct cgatcagctt tctggccgcg accgagtctt
     2341 ttctggagta ggagacaaac agctttgcca taattcccta tttcccatca attctaactg
     2401 aaatacaaaa ttcgctgaat ttcgttttta tggtaaaaaa ggcgaatgct aaacgactcc
     2461 aaccaattct cccgtgaagc ctgggacgcc aatgccgagc actgggattc gcgcatgggt
     2521 gacgaaggca acgatttttt caacctgctg tgttggccgg ttctcgcttc ttttctagac
     2581 gttcagcctg gccagcgtat cctggatatt gcttgcggca acgggctcac ctcgcgtagg
     2641 ctcgccgcgc tgggcatccg ggtgacagcc ttcgatttct cggcgaactt aattgaactt
     2701 gccaaagccc ggcccaaccc ggaatcccga attgactatc atgtcattga tgccacagat
     2761 gaacaggcgc tgttgaagct cagagatcaa cagttcgatt cagctctttc caatatggcg
     2821 ctattcgata tgcctgatat cgagccactt tttcgagtgc ttcctaaact tctcaagcca
     2881 aacggtgttt tcgccttctc cctgatgcat ccggctttca ataatccgtc ggcgactttg
     2941 cttgcagaag aatgggatga tggtcagatt caaacgcgtt acgcggtaaa agtgccaagg
     3001 tatatcaatc aatttcagtc gcaggggctt gcacttcgga atcaacccaa gccacagctt
     3061 tattttcatc gcccgatcag gtattacatg aacatcgctt tccagaatgg attcgtactc
     3121 gacgggtttg aagaacgcgc ctttccaccg gaccatccgc aaacggccac gctgggctgg
     3181 ggaggaaagt tcagtgaaat ccctccggca ttaattgcac ggatgagggt gcgctaggtc
     3241 tcataaaaaa atctccggaa ttttcggaga tttttctttt catgcttatg aagccacttt
     3301 ttcaaaaggc ttcaacgtga caggcggaag gctttgttca atggcaaccc gattaggctc
     3361 aaaccattca ggcagtttga gcgattcgcc cagcgactca accggttcat ctaacgcaaa
     3421 acccggtgtg tccgtcgcga tctcgaacaa cacaccgccc agctcacgga aataaatgga
     3481 gtggaagtaa ctacgatcca ttacctcggt gacgcggaag ccagcctggc ggatcgcatt
     3541 ttgataatcc aactgttttt catcggtggg aacgcggaat gcaatgtggt gaatggaacc
     3601 tgcaccaaaa ccagccggcg tctttccggg gcgatgaacg atatccacga tcggaccgga
     3661 aacatttgag tcgccgacga aacgataacg atcgctctcg ttccccgccg cgcgatatcc
     3721 catattcttt gtgagcaaat cggcagttgg ctctacttca tcgagccaca atgtgacgct
     3781 gtgaaagcct tgcaacgcgt acgactgtgg aatgggtccc gcctcccaaa atcggacagg
     3841 cgcggcagag gcggtctcga ccaactccac gcgcatgctg tcggggtcat cgaaagccag
     3901 cacatcctca ccaaatcgtt tctcgatggc ttttggggta ataccattct gtttcaggtg
     3961 ctcctgccag aaaccaagcg acccggctgg cacgttgtag gccaacgcgt tggtctctcc
     4021 gttgccgcgt atgccgcgcc gcatgttggg ccaggcaaag aacgtcagga tgcttccggg
     4081 tgagccggct tcatctccaa agtaaaagtg ataggtgtca ggtgaatcga aattcactgt
     4141 gcgcttgacg aacctctgcc ccagaacgtt acgataaaaa tcgatgttac gctgcgggtc
     4201 gcgcgccacg gcggtcacat gatgcaatcc tttgatcggt tccataagta ctcctttgta
     4261 atttcagagt agcacataag gttcaatctt acattggaag aagatatgga gaatgtatat
     4321 ttaatataaa aagtcttcgg gttcaaatcc gaagactgat atcggcaagc tgagaatcaa
     4381 ccgtacaact ctttcctgaa aaattcaggc aaggcaaatg ccgcctggtg gatttcagga
     4441 ttgaggtatt tattctcgcc gggtgggtat ggactcttca atccattcgt ccagggcgta
     4501 tcggagacat acataaagcc aatgcctccg cccggataag tggggatgtt ggcgtaataa
     4561 taggccgggt ttttcgtcag ctctttggct gatttatata tctctttgat caattcagag
     4621 tcgaagaagg gttgttcaca ttgtgtagca gcagcgccgc ccggaaccag cgcgcggacg
     4681 atcaactggt agaactcatt ggagaacaaa cgctctgcca ttccgatcgg atcagtggta
     4741 tcggaaatga tcacatcata ctgaccggga ttctcttcca ggaagccaaa cgcgtcccgt
     4801 acgagcagtt tggcgcgagg gtccatccat gggtcaccaa aagcagccgt gaaatgttcg
     4861 cgcgacaaat caaccacgcg ttgatccagt tcacagacca ccacttctcc tacagatgga
     4921 tatctcaaca cttgttgcaa ggagccacca tccccacccc cgacgatgag cacacgcttc
     4981 ggctgcccaa ctgccagcat cggcacatgc acgatcattt cgtgatagcc catgttatcc
     5041 cgctcagtca tttgaatgat gccgtcgaga atcatcgcat tgccgaaaaa ttcggtttcg
     5101 acaaattgca gatgctgata ttgcgtttgt tcatcggcaa ggacacgttt gatgcgaatc
     5161 cctttgcgat agtaagggtg taattcttct gtaaaccaaa tactcataag cgtagcaatc
     5221 ctgtggatat gcgaagtaat cctgtggata tgcgaagtaa tcctgtggat aagcgtctat
     5281 acagcgatcg gcatagattg aggttcaatg gcagcttcgc gatccagctt gcgaagcgca
     5341 aaatcgtccg cgccaaaagc ctctttgagg cgcatcagca aaggcatcat atccttgcca
     5401 atcgcacagg taaacaggtc aatggcgcaa tagccgtatt caggccaggc atgcaaactt
     5461 gcatgcgact ctgcgagaag caaaaaacaa gtaaagccat gggggctgaa cctgtaatag
     5521 ccctcatcta caaccgtcaa gccactttca cgcacggctt gcgcaaacag tgagtaggat
     5581 ctctcactat ccatgagaat ctcagggttg cagtcggaga gatcaaaaat ataatgctct
     5641 cccagtttca aagtcgcgtt cactcacacc tccggtgtta aaagataaag aacccatccg
     5701 acgacgccta cttttcattt caaaacgatc cgcaagatgt gtgccttgcg taacgagtga
     5761 aacggtcgtc ggatagactc tgttggggca tttatactat gtcctgcctg attgtcaatc
     5821 tttttctaag tttttctctc ataataggcg aacatgatct atctgctcat cgagtttctc
     5881 gacgaactgg tctttggtgt aggtgaaatt gcctggccac ttatccgcga cgatctccat
     5941 ctgacctata cacaaattgg tctgttgttg agcatgcccg ggatcatggc tgccttcatc
     6001 gagccattta ttgggatcct gggcgatgtg tggaaacgtc ggctgctcat catttttgga
     6061 ggattgcttt tcacgctttc ccttgtaatg acgagcgtga gctactcctt catttttcta
     6121 ctttcttcac tcatcctttt caatccctcg tcgggcgcgt tcgtcaatct ctcgcaagcc
     6181 aatctcatgg attcagaccc gactcgtcac gaacaaaaca tggctcgctg gacgtttgcc
     6241 ggttccattg gcgttctggc aggtcctctt cttctcgggc tgtttgttta tgttgggctt
     6301 ggctggcgcg ggacgtatgc tttccttgcc tttctctcga ccctttgcct gtttgcggca
     6361 cttcggtatt tgcctcctga aaaaaattca tctctaccgt tcccatcatt gcgagtaatc
     6421 ttcgatggct tccgcgcagc cttctctgcc ctcaaacgca gggaagtctg gcgctggctt
     6481 ctgctgcttg aatttgcaga cctgatcatg gatgtctttc tcagttacat ggcgctttac
     6541 tttgtggatg ttggacgtgt cactcaagcc caggcaggta ttgcggtaac gatctggc
//
LOCUS       DASTJP010000070         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1516688, whole genome shotgun sequence.
ACCESSION   DASTJP010000070 DASTJP010000000
VERSION     DASTJP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516688"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1058)
                     /locus_tag="VFQ23_02135"
     CDS             complement(252..1058)
                     /locus_tag="VFQ23_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9905401.1"
                     /translation="MKNINLIFNFIGVAVVDWQPAFQFFTQTLGMKYQLESRYGDWAM
                     LGGGWDAYYKDGSRSAIFELFDGGQAVTERYWGLNQGIRPGFHVSNLQPIVDRLDIPF
                     TLAKRPWGNTAEFISTEGIRFAFAEIPGTPFSDDLSVPYIGHAAIKCANFELMQHFYE
                     DVLGLIRVDAAAEYALFSQPDDHPFIVLEPGGSPSAIHQHDNSEINDAVRASPVFMSL
                     MTTDVQAAYIYLQHKAVTVLRETMSHEDWGGTDFHIADPDGNRIQVVQYG"
     gene            1241..>2035
                     /locus_tag="VFQ23_02140"
     CDS             1241..>2035
                     /locus_tag="VFQ23_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9905402.1"
                     /translation="MSSHRCSNCHTENRAEARFCKRCGFWLLPNCPFCNSSLPEAAVF
                     CDHCGRHLNLQLGNIAQTPGFQPAGAAVTSRAPREVPTPGPHGVPQKETKREPPKLAV
                     TNSELHQYIPEELMSKLQAARDKGGMVGERRVVTMLFCDLKGSTAAAEGLDPEDWTEI
                     MNGAFAQMIKPVYQYEGIIARLMGDALLAFFGAPIAHEDDPRRAILAGLDIVQAIKPY
                     REQIQERYGVDFDVRVGINTGLVVVGAVGSDLRMEYSALGDAINLAA"
BASE COUNT          527 a          493 c          518 g          497 t
ORIGIN      
        1 gatacgaacg agttccaagt acgataggta ataactatac ggtatttatc aagtttccag
       61 attaatatcg ggagagagtt ttgcaggatt gaatccgacg ctttgagggt ctcgctcagc
      121 ctgcacaagt ggaggcctcc cactaacccc ggcagaaggg tctgcatgag tcgatgctgg
      181 tattgattga agttatatac atgtcgtact tgagatggat gaggttattg tgcgagggtg
      241 cgaaagtaag attagccata ttgcacgacc tgaatcctat tgccatccgg gtcagcgatg
      301 tggaaatctg tgccgcccca atcctcatga gacatggttt cgcggaggac tgtcacggct
      361 ttgtgttgta ggtaaatgta ggcagcttga acgtctgttg tcatcaggct catgaaaacg
      421 ggggaagctc gcacggcgtc atttatctct gaattgtcat gttgatgtat tgccgatggg
      481 ctgccgccgg gctccagaac aatgaaggga tggtcatcgg gttgagaaaa cagggcatac
      541 tctgcagccg catcaactcg tatcagaccg agcacatctt catagaaatg ctgcattaat
      601 tcaaaattcg cacatttgat cgctgcatgt ccaatatagg gaactgagag atcgtcacta
      661 aagggcgtgc cgggaatctc ggcaaacgca aaacgtatcc cttcggtgct gataaactcc
      721 gccgtatttc cccatggacg tttcgctaac gtaaacggaa tatccagtct atctacaata
      781 ggctgcaaat tcgaaacatg aaagccgggc cgaatcccct gattcaatcc ccaatatcgt
      841 tcggtcactg cctgcccgcc atcgaaaagt tcaaagatgg cactccgact tccgtcctta
      901 tagtaggcat cccagccacc acccaacatt gcccaatcgc catacctgga ctccaattgg
      961 tacttcatgc ccagtgtttg ggtaaaaaat tgaaaggcgg gttgccagtc caccacagcg
     1021 acaccgatga aattgaaaat cagattgata ttcttcatca atagaagccc tccgtttctc
     1081 caattatcac aaatgacttg gttatgtaac agcctctaaa gagcggttaa atttttcagc
     1141 gtatccgtca ggcggacttg cgcgttaaat gcaatttggt ttggcagtag atcgagtgat
     1201 agggggatga aaatctgata tattatccat aggcaaattc atgagtagcc atcgctgttc
     1261 caattgccat actgaaaatc gggcggaagc caggttctgc aaacgctgtg gattctggct
     1321 tttgccgaat tgtcccttct gtaattcatc cttaccagag gcagcagtct tttgtgatca
     1381 ctgcgggcga cacctcaatc tccagctcgg aaatatcgct caaactccgg gctttcaacc
     1441 cgccggtgcc gcagtgacgt caagggcacc tcgcgaagta cccacacctg gtccacatgg
     1501 cgttccacaa aaggaaacga agcgcgagcc tccaaagctg gcagttacaa actctgaact
     1561 tcatcaatat atccctgagg agttgatgag taaactgcag gcggcgcgag ataaaggcgg
     1621 aatggtcggc gaacgacgcg tggtcaccat gctgttttgt gacctaaaag gttccacggc
     1681 cgccgccgaa gggcttgacc ctgaagattg gacagagatc atgaatggcg ccttcgctca
     1741 aatgatcaaa cccgtctatc aatatgaagg aatcattgcc agattgatgg gcgatgcact
     1801 tcttgcattc tttggtgcgc cgatcgcaca tgaagatgac ccgcggcgtg ccattttggc
     1861 cgggctggat atcgtccagg ccatcaaacc ctatcgtgag cagatccagg aaagatatgg
     1921 tgtcgatttc gacgtacgcg tgggaataaa cacgggtctg gtcgtggtag gcgccgtcgg
     1981 ttcggatttg cggatggaat attcggcgct tggagatgcg atcaatctgg cagca
//
LOCUS       DASTJP010000071        24111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1094383, whole genome shotgun sequence.
ACCESSION   DASTJP010000071 DASTJP010000000
VERSION     DASTJP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24111
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094383"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFQ23_02145"
     CDS             <1..191
                     /locus_tag="VFQ23_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905403.1"
                     /translation="YGQDNRSPISADTKHVLYVAYAPAGVPEEIVDMQLQKIVENVRL
                     FSQVVTVEQHRLIKADAD"
     gene            226..558
                     /locus_tag="VFQ23_02150"
     CDS             226..558
                     /locus_tag="VFQ23_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9905404.1"
                     /translation="MKIIQMKDIKAPHGEVATFQGLEHEANISFFIVNFSPGKGPGKH
                     RHPYEETFIILDGEIEAVVDGETQVLGSDTIAIIPAGTWHEFKNRSAEKVSMVNIHPV
                     PKMITEWG"
     gene            796..3270
                     /locus_tag="VFQ23_02155"
     CDS             796..3270
                     /locus_tag="VFQ23_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9905405.1"
                     /translation="MMRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPG
                     GVIPQILEKLSVSAEALTERLDATLRASPKANIFGGGAGQIFITPRVKRIIDLANEEA
                     NRLKDEYISTEHIFLAILTERNTPAARILESAGLTRDRVYTAIQDLRGGQRVTDPQAE
                     SRYRTLEKYSRDLTQLAREGKLDPVIGRDNEILRLIQILSRRTKNNPVLIGEAGVGKT
                     AIAEGLAQQIANNDVPEILSGKRVVQLDLGAMIAGSRFRGEFEERLKAVMEEVQRSQG
                     DIIMMIDELHTVVGAGAAQGAMDASNMLKPALARGELQTIGATTLDEYHKYIEKDAAL
                     ERRFAPIYVEEPTVEDTIKMLMGLRDRYEAHHKVRFADEALVAAARLADRYVTDRRLP
                     DKAIDLMDEAAAKLRVALYSMPPDLKAMKSEIEKLQAEEEQAGLNRDYERAAQKKSER
                     LRLEEEYKEKRDKWESEHQLDEVVDVDDIASVVNQWTGIPLTQMLESESEKLLHMEAR
                     LHERIIGQEEAIHAVSDAIRRARSGLKDPSRPIGSFIFIGPSGVGKTELAKALAWFMF
                     DDEEALVRIDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVVLFDEIEK
                     AHPEVWNALLQILDDGRLTDGQGNIVDFRNTVLIMTSNLGTEYVRKGGTLGFLQPKAS
                     SEEREAHDKVEKALRGAFRPEFLNRIDEIIMFSPLSIEQMEEIVVLQMKEVQDRLNEY
                     NITVELTDAARKWLAKEGYDPAFGARPLRRAIQKYVESPLSVELLAGKYKDGGTVIVN
                     VDEEKNKVVFETSELVTKKSKQHADA"
     gene            3391..5394
                     /locus_tag="VFQ23_02160"
     CDS             3391..5394
                     /locus_tag="VFQ23_02160"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905406.1"
                     /translation="MEANSNQSSSVPKLVGAIVAILVCCSCVVIAAAGFVMYSASRQT
                     PTDFATLIPPIDGFVTPPPTPELERPTADPISSETLETLENTIVPDNDPYELACRLQN
                     ICNVATTVPSKQFQIGDQQTFWVSNTDDNENFQVTATLRYITDHMYFWIENGVSYLDS
                     DVKRLGDTFENEMYPTNREFFGSERSPGIDNDPRIYILYAGGTGASNGGYFSSPDEYN
                     PLVLEYSNGHEMFVFNADNIDLATETAYSVLAHEFQHMIHFNTDANEDTWMNEGFSMV
                     AELLNGYSVNYDYVYVGEPDLGLTDWSASASNIPHYGQSFLYLTYFLDRFGEEATKSV
                     IKHSENGLVSIDETLKELNTTDAVSGAAITADDVFMDWAATMWLMDPSVGDGRYDYHN
                     YPDAPTITVSESVPNCPQSTSGSVNQYGLDYFSISCEGDYTLSFSGSTVAGLIPADAH
                     SGKYAFWSNKGDSSNMTLTRDFDFTGVSAPITLSYSVWYDLETDYDYVFVEASTNGET
                     WEILTTPSCTTEDPSGNSYGCGYNGQTNSWVTEEVDLSQFAGQNVQIRFDYVTDAAVN
                     GEGFLVDDVQVDAINYQTDFEADEGGWEAAGFARIENVLPQTYRLSLITRGDTTTVTH
                     IPLNADQTAEIPLSLQPGEEAILIVTGTTRFTRLPAAYQIEIK"
     gene            complement(5469..6704)
                     /locus_tag="VFQ23_02165"
     CDS             complement(5469..6704)
                     /locus_tag="VFQ23_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905407.1"
                     /translation="MDFKRIARKITWTLFITQSLASAGFIAAATINPILGAKLASDRS
                     LATLPTAVYLLSGALSASAWGYLMDRIGRRNSIVLGLLIGVVGNALVLAAIQMESFLL
                     VLVGLLLMGITNAAVTLGRFAAAEVNTPEQRGRAISAVVLGGVIGTILSRVLAVPVGN
                     FAVSIGLDELAGAYITTLVLFAVASILVFTSLRPDPRDVGKELAKLYPEAMPHGGVSR
                     PIFQILREPAAITAVSAMVLGQMVMVAIMVVTSLHMEDHQHARSDIYSVISAHTFGMF
                     APSIFSGWLLDKVGRGKMILIGALTLLLACATAPISPDVLPLGVSLFLLGLGWNFCFV
                     GGSTLLSDQLSPVERSRTQGTNDLLVGLASAFISLSSGFIFDSAGYTVMSIVAGVFSL
                     IPLALALSFVRKRAVSVGA"
     gene            6829..7683
                     /locus_tag="VFQ23_02170"
     CDS             6829..7683
                     /locus_tag="VFQ23_02170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905408.1"
                     /translation="MDFNTHFATEYGLPELVEILNRGFENYFIPIQFNMSQFSTMLRK
                     DSIDLDSSRILLIDGEPAGIALIARRGWTSRLVAMGISMPMRGKGAGLWFMKNLIEES
                     RQRNDREMVLEVIEQNESAVKLYQKTGFQIVRRLIGFIHTSPAGESSSDVEELDLREA
                     GRLITLFGFSDLPWQLSGETIAHMNPPARAYRKDQAYAVISNPDAEHVVIYSLLVHPD
                     ARGDGLGLDMLKSLIAKHTGKTWHVPAILPEELGKLYEEAGFKKENLSQWQMKLTLIP
                     NTSVKGES"
     gene            7770..10982
                     /locus_tag="VFQ23_02175"
     CDS             7770..10982
                     /locus_tag="VFQ23_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9905409.1"
                     /translation="MQSALKPFSSERKTIGVFASQVGRAWGAEFMAGITHAAEANNVN
                     LVHFIGGKLMSFTAPGESKPSFGLYDLVKPDQFDGLLLTTDVSYGTSAHDLETFTKVY
                     GTLPIVTQSVDIPGASMFVPDNIEGMRAVVRHLIEDHGYKRIAFIRGIEGQIDAEQRF
                     QSYKDELKAHDLRFDENFVVRGDYTPESGRAAIFTLLDERKLRFQAVVAANDRMAFGA
                     LDALQERGVRVPDDVAVTGFDDLREAQSTGVPLTTVRQSFYTAGKHALETLLRRINGE
                     AVPAVTITPTQLLVRWSCGCLPENVRQAAVAPRDVAKTARLENKREAALKALLDSAGV
                     TEQDPALLQFRDAFGRAWDGFLLTLNDKSTNDEFLKTINSMIELMQKQGLPPALWHNV
                     ISMMRRYALGGITSHTTMLKAENLFQQIRLLAGELSQRAQAYRRLVLEQQENILQGFS
                     TSMAPAISLNDIGAAISEHFPSMGIGRWYVMFYSDVTAPQSISSPPPESYSLLLQYEG
                     ARFDIPTRRASIGTGQLVPRGKTPQDYRYTAVIMPLSLAKNRFGFMWVEMGSQDWEIY
                     VRIRNLVSSALLRTMLVQQKEQAQHKIERLLEEARERAAELAVAKEWAEVTAAENAKL
                     YSSEQMRREAAEALAKSSRLLSTLGTVGEVPQQIVTQLSQLIKNERSALFLEDVNGIP
                     RLLAHQGLPKESPAKELSYKINGTNIYHIVAKQSESMVIGDVKNMQGWSQSLWLPEDR
                     SWLGVPLYSKNKVAGMLTLSRKNPAAFNQDDVLLATTFAVQASIALDNARLYDELTRF
                     NQMMERMVEQRVDELNSAYGTLEKLDKNKSDFIQVAAHELRTPLTVIKGYMGMLKSAQ
                     AIQGNPTLMQAMDGVLQGTDRLHQIVNSMLDVARLENQVLSPHLEIVTLGPILRMIQK
                     DYADDLTARNLRLEIDPRVNSIPAILADSELLQKAIDNVVVNAIKFTPDGGSINISAD
                     TVKMDGRSDICEIRIQDTGIGIDPANHAIVFEKLYQLGKVELHSSGKTKFKGGGPGLG
                     LAIAAGIVKAHKGKIWVESSGYDEEKLPGSTFFIQIPLAK"
     gene            complement(11058..11768)
                     /locus_tag="VFQ23_02180"
     CDS             complement(11058..11768)
                     /locus_tag="VFQ23_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905410.1"
                     /translation="MNNSPNTGPRGPSPLLPFRGLSQRGLLSLLMLLLSIGGLMFALL
                     GGARLILDIFSVGLLNSINGIATKLIVIGLAYAVGWLSAMFAIRVFGNLVLPFIINIF
                     IWGCLIGVCSLYVLILQRLYVQAYGDLQFWAYLLTVVAGLGAMIGLHLIIEDHDLRPF
                     SVPLLIINLIQLGLIVFRYVFTTGANPAYLLNDIFFFLAMSAFSIFMLVHIGLLAPIR
                     AQLTQYFDKNSKALRNEN"
     gene            complement(11969..13144)
                     /locus_tag="VFQ23_02185"
     CDS             complement(11969..13144)
                     /locus_tag="VFQ23_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9905411.1"
                     /translation="MSNRKRVIVVGGGSFGISAAIELHQRGWEVDVFDPGPIPHPLAA
                     TTDISKILRMDYGADEDYMVLMEQAFDIWDEWNRLWKEPLYHETGFVIMKREEMQPGS
                     FEYESYQLLKKRGHNVERLDSQELKRRFPAWNAELYSDGYFNPRAGWVESGRVLEWLY
                     GEAQRIGVRFRVGEKFSHLLDQDSRVIGIQTEDSEKHYAEYVIVAAGAWTPHLLPHLS
                     DVLWTVFQPVFHFQVPEMEIFSPPNFVVWAADIANTGWYGFTAVQDHRFKIANHGTGW
                     RFHPDEPRLMPEDQEEKFRDFLQGTFPALATAPVLFNRLCPYCDSWDGNLWIDHDPDR
                     PGLMVASGDSGHAFKFTPLLGRIISDVLERKPNRVASKFAWRARGTITTEDARYSKE"
     gene            complement(13154..13444)
                     /locus_tag="VFQ23_02190"
     CDS             complement(13154..13444)
                     /locus_tag="VFQ23_02190"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HET9905412.1"
                     /translation="MEETTDINRELMLDANAAAGLLYEIFGMDMTTALGECFSCGNQA
                     EMGTLLAFTHGPGVVLRCSMCEHVVLRIVKTPDEIFLDARGAVFIRLKQQQQ"
     gene            complement(13514..14254)
                     /locus_tag="VFQ23_02195"
     CDS             complement(13514..14254)
                     /locus_tag="VFQ23_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HET9905413.1"
                     /translation="MKPTINWQIASIKDIKPETANVKSFTLTLPSWMRHRAGQHYDIR
                     LTAEDGYQTQRSYSIASEPEREGEVDITVERINDGEVSAYLHDVLVPGDRLEARGPIG
                     GYFVWEAAMTEPLLLIAGGSGVVPLMSMIRHHAAAGARNPISLLYSARNFEDIIYYNE
                     LEKLGAANNALQILYTLTRSQPAGWKGYARRIDQQMLKEIAQPLGKTVQVFICGPTLL
                     VEATANSLVKIGVPSSQIKTERFGPTGG"
     gene            complement(14369..14989)
                     /locus_tag="VFQ23_02200"
     CDS             complement(14369..14989)
                     /locus_tag="VFQ23_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HET9905414.1"
                     /translation="MTEERFVSRGFVGKRRGGEKKDRIPPGQYLTNDFPVLSAGPTPY
                     TPLDRWTFSIEGLVKEKVSWTWEEFLKLPMQKYVVDISCVTKWTKLDMKWEGVSLDTL
                     LEHVELDRKAAFVTAYSDGGYTTNMPLTDIINGQSFIAWQYDDKPLRPEHGGPVRMVV
                     PHLYFWKSAKWVRGIYLTEKDKPGFWESAGYHNRGDPWKEQRYWND"
     gene            complement(15062..16174)
                     /locus_tag="VFQ23_02205"
     CDS             complement(15062..16174)
                     /locus_tag="VFQ23_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9905415.1"
                     /translation="MTKTNPNFVLDDYSKYILLDYRQKLLRVLLVTSMVILFGLTIFD
                     RMGWLPSTLIELEGINPVSTGITITVFLILLWMNQKGYVPLVGWIFCLFLFTVILVSY
                     GTLDFPLRFFVIAWLVVIASFVIQPWAAFLFTGASIISFWWLPAQSTQASNFGGFPLV
                     ALIIIAIGAYIVSRILNKSISAAVHAYDETIQGWANALELRNSESMGHSQRVLNLTLS
                     LARKLGIRGPDLIQVRRGVLLHDIGKMGIPDAILHKPGALTEEEWGLIRKHPDYARDY
                     FSGISYLSPAMDIPFFHHEKWDGTGYPHGLQGDHIPLPARIFAVADVWDVLMSDRSYR
                     DAWTREKALQYIHEQSGKHFDPRIVEMFVRLLEKHK"
     gene            complement(16255..17655)
                     /gene="lpdA"
                     /locus_tag="VFQ23_02210"
     CDS             complement(16255..17655)
                     /gene="lpdA"
                     /locus_tag="VFQ23_02210"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET9905416.1"
                     /translation="MAENFDVVVIGAGPAGYVAAIRAAQLKQKVAIVDKQWLGGVCLN
                     VGCIPSKSLLKNAEIAHTLRERGKDFGFSFDNLKLDYPVAVKRSRQNSDRLVKGVGFL
                     MRKNNIAVFMGEAKLKSKDTLTVTDKDGKATEIKAKNIIIATGATAAVPGAWKVDGEK
                     VVTYQEAILQEKLPKSVIVIGSGAIGVEFSTVWNSYGVDVTIVEMLPRLVPLEDEEIS
                     KDLKKEFEKRGIKCLVGNKVESVETTKTGVKVKVSAEGKETTLEAEQALVAIGFRPNS
                     KGFGLEEAGVKINERGLVEINEKMQTSVPNIYAIGDVTGKLMLAHVGSAMGIVCAENI
                     AGAETIVLDYDMMPRATYSHPQIASFGYTEAQAKERGYGIKVGRFPFQANGKALGLGD
                     YAGWVKIIVDEKYGEILGAHMIGPEVTELLPELTLAHLMELTPHEIARNVHAHPSLSE
                     VIMEAAHGAEGQPIHI"
     gene            17750..19306
                     /locus_tag="VFQ23_02215"
     CDS             17750..19306
                     /locus_tag="VFQ23_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905417.1"
                     /translation="MTMMETLRSRKLIIPMLVVFVALLVQTSWAEEDAFITLRTSDNL
                     IHGYGPRWNVAERVQTFTNPFWMFLLAGTHAIIKNPHITLILLSIFVSVLAMLIMLVY
                     LPQTNFGVVLAFSILVLSKAFVDFSTSGLENPASHLLIVCFAAFYLRSEKPLSERNLF
                     FVSLFAGLAAFNRLDLFLFCLPALVATLFAQFNRRNILIMLAGLSPVLVWEIFAVIYY
                     GFPVPNTYYAKLHTGVPFNESITQGILYFINSFGWDPITLTIVFSAIGLPLVSRYHER
                     ILLALGIVLYLGYILYIGGDFMSGRFFSTPLMISVALLVHRVEDATTLEKYAWISLVL
                     LLGLILAPLKSFNNPIESDVISFDSTAGIADERFGYYRYSNLLLFSRDQDFQLYEFAQ
                     YGIYLRREKIPLATIPAIGMVGYYAGPGTYIIDKLGLGDPLMARLKRTDPKNWQIAHI
                     ERNVPDGYGDSIENNENRINDPALAEYYDKLLLITRGDIWSAQRWEAIWKMNTGQYDY
                     LLERYAKSQK"
     gene            complement(19316..19984)
                     /locus_tag="VFQ23_02220"
     CDS             complement(19316..19984)
                     /locus_tag="VFQ23_02220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9905418.1"
                     /translation="MSVRKSEIKLDVDGQPVNAYLAAPESGGPGVLVLHAWWGLKPFF
                     KQICDRLAEQGFVALAPDMRNGQIAETIDSAKELMEKSDNQVIGDIVMTAKDYLLAHS
                     GGEKIGVVGFSMGAAWSLVVAGYDPDKIAATVLFYGIGETEFSKVKSKILGHFSDNDE
                     WEPMEGVRWLEQSLKDASVDSTFHIYPAVAHWFVEEDRPEYDSAASDLAWNRTFEFLK
                     QNLG"
     gene            complement(20032..20502)
                     /locus_tag="VFQ23_02225"
     CDS             complement(20032..20502)
                     /locus_tag="VFQ23_02225"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9905419.1"
                     /translation="MATDKEHPDHASVKIHPPVLLLLHMGAVYLLNRLFPFVPPDPLI
                     WVGYLLVVVGLGLAFSAVIRFIRARTTLDPHGSVSGIVKDGVYRFSRNPIYLGFVCLL
                     IGFPFAFGSYWGLILSPLFIFLMNTLVIQPEEAYLEKKFGEAYTSYKSRARRWL"
     gene            complement(20504..21739)
                     /locus_tag="VFQ23_02230"
     CDS             complement(20504..21739)
                     /locus_tag="VFQ23_02230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9905420.1"
                     /translation="MATKVFVPLLGEGVEEVTVIKWLKQEGDSVNELEPLLEVNTDKV
                     DTEIPAPASGTVLKIMAEEGAPAKVGTVLAFIGKPGEVVDSGQSSVGSEKPLPVSSQP
                     VTVHDQASTDNPQTPTDNKDIGFISPVVAKIAAEHGVNLQNVTGTGLNGRITKNDVLN
                     FVETGKSTFSVSQPSSPSAPTITPVAGDQLIKHTVIRKQIAEHMVMSKHTSPHVLTVM
                     EADLSRVGKHRSANKEIFARDGINLTYTAYFMTAIVAGLKNYPIVNSSWTEEGVLVHK
                     AVNLGMATSLGEDGLIVPVIKDADNLSLLAMARAVNDLANRARAKKLQPDDVKGGTFT
                     LTNHGISGSLFAYPVINQPQCGILGIGAMQKRVVVIDDAIAIRPMVYLSFVFDHRILD
                     GARADAFLMKVKETLEGWS"
     gene            complement(21780..22157)
                     /locus_tag="VFQ23_02235"
     CDS             complement(21780..22157)
                     /locus_tag="VFQ23_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9905421.1"
                     /translation="MDNNKVAARNYRELIVWQEGIKLAKAVYKLTEKFPRQETYALAD
                     QIRRAVVSVPSNIAEGQARKAPGDFRRFLYFSLGSLAEVDTQLILAKEFGYLSKEDVD
                     VIDEQIQGLRKKMYALINSLPAR"
     gene            complement(22184..>24111)
                     /locus_tag="VFQ23_02240"
     CDS             complement(22184..>24111)
                     /locus_tag="VFQ23_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9905422.1"
                     /translation="GVEELKQFTQFSKNGNEVAFVSIGNASSAEGMFWESINAIGVLH
                     APAVISIYDDGYGISVPNEHQMVKGNIGELLKGFQRAPDSNEGFDVYCVKAWDYPALL
                     QTYARATETAREHHIPALVHVTEVTQPQGHSTSGSHERYKSPARLRFEEEFDGLLKMR
                     EWMLENRVIKEPELLAVEKEDHDTVEGIRKLAWDAYLAPIIEERSQVMDMFDEIAGSS
                     KHASELNDIKERLSALPAPLRRDVHSAAHEALRILRDELHPSKQVLVEWKNEQDAVNE
                     ERYGSHLYSGRAVTVKEVKPVYSSSSPTMMGFEVMNMAFDAILSRDPRVIAFGEDVGF
                     LGDVNQGFKGMQEKYGALRVSDTGIREATILGQAIGMAMRGLRPICEIQYLDYVLYAL
                     QIMSDDLANLHWRTVGGQSAPVIVRTRGHRLEGIWHSGSLMAGLIHLVRGINVLVPRD
                     MTRAAGFYNTLLKADEPAVVVEVLNGYRAKERVPDNIGEFTVPLGVPEVIREGANADL
                     TLVTYGACCRIALDAAEKLSRVGIEVEVIDVQSLLPFDIHGKIVESLKKTNRILFVDE
                     DVPGGASAYMLQEVIEKQGGYFHLDSPARTLSGKAHRPAYGSDGDYWSKPNAETIFDT
                     VYEMMNEVDPAAYPPLR"
BASE COUNT         6188 a         6308 c         5782 g         5833 t
ORIGIN      
        1 tttatggaca ggataatcgc tcacccattt ctgcagatac caaacacgtg ctttatgttg
       61 catatgcccc tgctggcgtt ccagaagaaa tcgtggatat gcaactgcag aaaattgtag
      121 aaaacgtccg cttgttctca caggtcgtta ctgttgagca acaccgttta atcaaggcag
      181 atgccgacta gcatcgtgcg acaataccac catggaggaa attcaatgaa gatcatccag
      241 atgaaggata tcaaagctcc gcatggcgag gttgcaacct tccaggggct tgaacacgaa
      301 gccaatattt ccttcttcat cgtaaatttc tctccgggaa aaggtcccgg taaacaccgc
      361 cacccttatg aagagacatt catcattctg gatggagaaa tagaagctgt tgttgacgga
      421 gagactcagg tactaggcag tgacaccatt gcgattatcc ccgccgggac atggcatgag
      481 ttcaagaatc gatcggcaga gaaagtgtcc atggtaaata ttcatcccgt tccgaagatg
      541 atcacagagt ggggataggg tgtgttaaag ctcgaaacga ggttcgcgta atgcaaatct
      601 gcacatcaaa tctaatactc ttctaacgcc gttccaacaa ttcggtaacc tggttttgtt
      661 ataatgcccc tattcggaga cctgacaggt ctccgctcac gcttgacagg gcctcatttc
      721 tttccgcatg ctgattagga cgggaaagat gcggatcaga cctgtcaggt cttgaactaa
      781 aaaggaacat tccccatgat gagatttgac agatttacag aaagagcaca ggaagccgca
      841 caacgcgctg ccgagatcat ccagcgctac ggccataatc aaatcgatac cgaacacatc
      901 ctgctggcgc tcatcgagca gcccggtggt gtgattcccc agattttgga aaaactaagc
      961 gtcagcgccg aggccctcac cgagcggctc gacgccacct tgcgcgccag ccccaaagcc
     1021 aacattttcg gcggcggagc tgggcagatt tttatcaccc cccgcgtgaa gcggatcatt
     1081 gacctcgcca acgaggaagc caaccgcctc aaggatgagt atatctccac cgaacacatc
     1141 ttccttgcca tcctcaccga gcggaatacc cccgcagccc gcattttgga atccgccggg
     1201 ctgacgcgtg accgcgtcta taccgccatt caggatctgc gcggcgggca gcgcgtcacg
     1261 gaccctcagg ctgagtcccg ttaccggacg cttgagaagt attcccgcga cctcacccaa
     1321 ctggcgcgtg aaggcaagct cgaccccgtc atcggacgcg acaatgagat tttgcgtctg
     1381 attcaaattc tttcgcgccg caccaagaat aacccggttc tgatcggtga agctggtgtc
     1441 ggtaagactg ccattgcaga aggtctcgcg caacagattg cgaataacga cgtccctgag
     1501 attctctccg gcaaacgcgt tgttcagctg gatctcggcg cgatgatcgc tggttcacgc
     1561 ttccgtggtg agttcgaaga gcggctcaaa gccgtgatgg aagaagtcca gcgctcccag
     1621 ggcgatatca tcatgatgat tgatgagttg catactgtgg tcggcgcagg tgccgcacaa
     1681 ggcgccatgg atgcgtccaa catgctcaag cctgcactcg ctcgcggtga attacaaacg
     1741 attggtgcta ccaccctgga tgaatatcat aaatacatcg aaaaggatgc agcgcttgaa
     1801 cgacgttttg ctcctattta cgtggaagaa ccgacggtgg aagacaccat caaaatgctg
     1861 atgggcttac gcgaccggta cgaggcacac cacaaggtcc gttttgccga tgaggcgctc
     1921 gtcgctgcgg cccgattggc cgaccgttat gtcaccgatc gtcgcctgcc tgataaggca
     1981 attgacttaa tggacgaagc cgccgcgaaa ttacgcgtgg ctctatattc catgccgccc
     2041 gatctcaagg ccatgaagag tgaaattgaa aagctccagg cggaggaaga acaagccggc
     2101 ttgaatcgtg attacgaacg cgccgcgcag aagaaatctg agcgcctgcg actcgaagag
     2161 gaatacaaag agaagcgcga taaatgggag tctgaacacc agctggatga agtggtggat
     2221 gtggatgaca tcgcttcggt tgtgaatcaa tggaccggta tccctctcac gcagatgctg
     2281 gagagcgaat ctgaaaaact cctgcacatg gaagcacgct tgcacgaacg catcatcggg
     2341 caggaagagg ccatccacgc cgtatcggat gccatccgcc gtgcacgctc aggtctcaag
     2401 gacccctcac gaccgatcgg ctcatttatc ttcattggtc catcgggcgt cggtaagacc
     2461 gagctggcaa aagccctcgc ctggtttatg ttcgatgatg aagaggcgct ggtgcgcatt
     2521 gacatgtctg agtatcgcga acagcatacg gtctcgcgac tctttggtgc ccctccagga
     2581 tacgtggggt atgaggaagg cggtcaactc acggaagccg ttcgaagacg tccgtatcga
     2641 gtggtgctat ttgacgaaat cgaaaaggcc catcctgaag tctggaacgc cttactccaa
     2701 atattggatg atggtcgtct gacggatggt cagggcaaca ttgtggactt ccgcaacacg
     2761 gttctgatca tgacctcgaa cctgggtact gaatatgtgc gcaagggagg cacgctcggc
     2821 ttcctgcagc caaaggcatc cagcgaggag cgtgaggcac atgacaaggt tgagaaggca
     2881 ctgcgaggtg cgttccgccc tgagttcctc aaccgcatcg atgagatcat tatgttctcg
     2941 ccgctctcca tcgaacaaat ggaagagatc gtcgtccttc aaatgaagga agtgcaggat
     3001 cgcctgaacg aatacaacat cactgtcgaa ctgaccgatg ccgcacgtaa gtggctcgcc
     3061 aaggaagggt atgacccggc ctttggtgca cgtccgttac gccgtgctat tcagaaatat
     3121 gtagagagcc cgctttcagt ggaattactg gcaggcaagt ataaggacgg cggaaccgtg
     3181 atagtaaacg ttgatgaaga gaagaacaag gtcgtcttcg aaacgtccga actcgtaacg
     3241 aaaaagagta aacaacacgc agatgcgtga aacaaaacaa acgtaccaag caggggcgca
     3301 gcaatgctgc gcccctgctt tattttctaa ttccgcattc atgaataaaa ggtaagatgc
     3361 agcgagttta tttacaagga gatcattatt gtggaagcaa atagcaatca atcttcatcg
     3421 gttccaaaac ttgtaggggc gattgtagca attcttgtct gttgttcctg tgttgtgatc
     3481 gcggcagccg ggtttgtgat gtacagcgca tcccggcaaa ccccaaccga ttttgcgact
     3541 cttatccctc ccatcgatgg ctttgttacc ccgcctccta caccggaact cgaacgacct
     3601 actgcagatc ctatctcgtc agaaacatta gagacccttg aaaacacaat tgttcctgat
     3661 aatgatccgt acgaacttgc gtgtcgcctg caaaatattt gtaatgtcgc tacaacggtt
     3721 ccctcaaaac aatttcagat cggggaccag caaacatttt gggtatcgaa taccgatgac
     3781 aatgagaatt ttcaagtcac cgccacgctg cggtacatca ccgatcatat gtatttttgg
     3841 atcgagaacg gtgtcagtta cctggatagc gatgtaaagc gattgggtga tacgttcgag
     3901 aatgagatgt acccgaccaa ccgtgagttc ttcggtagtg agcgcagccc tggcatagat
     3961 aatgatccgc gtatctacat cctttacgca ggcggaacgg gtgcatcgaa tggcggctat
     4021 ttttcatccc ctgatgaata caatccgctt gtgctggaat actccaatgg tcatgagatg
     4081 ttcgtgttca atgccgataa cattgatctg gcaacagaaa cagcttacag tgtcctggca
     4141 catgagtttc agcatatgat ccacttcaat acggatgcga acgaggacac atggatgaac
     4201 gaagggtttt ccatggtagc cgaacttttg aatggatatt ccgttaatta cgattatgtg
     4261 tatgtgggtg aacccgatct cggactcaca gactggtcgg cctcggcttc caacattccg
     4321 cattatggtc aatcgttttt atatctcaca tactttttag accgattcgg tgaagaagcc
     4381 acaaagtctg tgataaagca cagcgaaaat gggctggtaa gtatagatga aacgctcaag
     4441 gaactaaata ccaccgacgc cgtctctgga gccgcaatta ccgccgatga tgtcttcatg
     4501 gattgggctg caacgatgtg gttgatggac ccatcagtcg gtgacggacg ttatgattat
     4561 cacaactatc cagatgcgcc aaccatcaca gtgtctgaat ccgttccaaa ttgtccgcag
     4621 tcgacgagtg gctctgtcaa ccagtatgga ctggattatt tctcgatcag ttgtgaaggg
     4681 gattacacgc tgagttttag cggttccacg gtagctggcc tcatccctgc agatgcccat
     4741 tcgggtaaat atgctttctg gtcgaacaaa ggtgattcat cgaatatgac cctcacgcgc
     4801 gacttcgatt tcaccggtgt gagtgcaccg atcactttgt cttactccgt gtggtatgac
     4861 cttgaaaccg attacgatta tgtctttgtg gaagcgtcta cgaatggtga aacctgggag
     4921 atactcacaa cgccttcctg taccacggaa gacccctctg gcaactcgta tggctgtggc
     4981 tataacggtc aaacgaacag ctgggtcacc gaagaggttg acctttctca gttcgccggg
     5041 cagaacgttc agattcgttt tgattacgtc acggatgcag cggttaacgg agaaggcttt
     5101 ttggtggatg acgtgcaggt ggatgccatc aactatcaga ctgattttga agccgacgag
     5161 ggaggctggg aggcggccgg atttgcacgc atcgaaaacg tcttgccgca gacctatcgc
     5221 ctcagcctta ttacccgagg tgatacgacc acggtgacgc atattccgtt aaatgccgac
     5281 cagaccgccg agattccact ctcactgcaa ccaggcgaag aagccatatt gatcgtgacc
     5341 ggcaccacac gctttacaag gctccccgct gcgtatcaga ttgaaattaa ataaatcccc
     5401 tctcccggcg ggagagggtt agggtgaggg cgaaacatcc cgcagaaaat attctgcggg
     5461 atgttgattc atgcaccgac cgaaacagct cttttccgca caaacgacaa tgccaaagca
     5521 agaggtatta acgagaatac tccggcgacg atggacatca ctgtataacc cgctgagtca
     5581 aagataaagc cgctgctcaa gctgataaaa gcagaagcaa gaccaacaag taaatcattc
     5641 gtcccttgtg tgcgtgagcg ctcgaccggc gagagttgat cggataatag ggtggaacct
     5701 cccacaaaac agaaattcca gcccaaccca agaagaaaaa gggaaactcc aagcggcaac
     5761 acgtctggtg aaattggcgc tgtggcgcat gccagcagca acgtgagcgc gccgatcaga
     5821 atcattttgc cacgaccaac tttgtccaat agccagcctg aaaaaatcga cggcgcaaac
     5881 ataccaaatg tgtgagctga gatcacggaa taaatatcac tccttgcatg ttgatgatct
     5941 tccatatgaa gcgaagtgac gaccatgatg gcaaccatca ccatttgccc cagcaccatc
     6001 gcgctgacag cagtgatcgc tgccggttcg cgcaaaattt gaaatatggg ccgagacacg
     6061 cctccatgtg gcatggcctc ggggtaaagc tttgccagtt cttttcccac atcgcgcgga
     6121 tcagggcgca gacttgtaaa gaccaggatg gatgccaccg cgaacaacac cagcgtggtg
     6181 atataggcac cggccagctc atccaggccg atacttacag caaagttccc cacggggacg
     6241 gccagcacgc gtgataatat ggtcccgatc accccaccca gcacaacagc agaaatggcc
     6301 cgcccacgct gttcaggagt attcacctct gccgcggcaa agcgtcctaa tgtgaccgca
     6361 gcatttgtaa tccccatcag taataaacca accagaacca gcaggaacga ttccatctgg
     6421 atggctgcta atacgagcgc attgccaacc acaccgatca acaatcccaa cacgatcgaa
     6481 ttccgccgac cgatgcggtc catcagataa ccccaggcag atgcagataa agctccactt
     6541 aacagatata cggctgtggg caaggtggcc agcgaacggt ctgatgctag ttttgctcca
     6601 aggatgggat tgatggttgc agcagcaatg aacccggcgg atgcaaggct ctgcgtgata
     6661 aacaatgtcc atgtgatttt tcttgcaatg cgcttgaaat ccatgatgaa aagggtcctc
     6721 tcgggttttg attatacgat ggacgcgggt agaatagaaa aaccatcttg taaagccaaa
     6781 gcatcgtttc acacgaacaa tgcttaccct ttcattcagg gaactattat ggatttcaat
     6841 acacactttg ccactgaata tggcttgcct gagcttgtcg agatcttgaa tcgcggtttt
     6901 gagaattatt tcattcccat tcagttcaat atgtcccaat tttccaccat gcttcgcaag
     6961 gacagcatcg atctcgactc gagccgtatt ctactcatcg acggagagcc cgcaggaatt
     7021 gcgttgatcg cccgccgcgg ttggacaagc cggctcgtag ccatgggcat ctcaatgccc
     7081 atgcgcggca aaggtgcagg gctgtggttt atgaaaaacc ttatcgagga gtcgcgccaa
     7141 cgcaacgacc gggaaatggt gctggaggtc attgaacaga atgagtcagc cgttaagttg
     7201 tatcagaaaa ccgggtttca aatcgtacga cggctgatcg gtttcattca tacgagccca
     7261 gccggagaaa gttcgagtga cgtagaagag ttggatctgc gcgaggcagg gcgtcttata
     7321 acattgtttg gattctctga cctcccctgg caactatctg gtgaaaccat cgctcatatg
     7381 aatccaccgg cacgtgccta tcgcaaagac caggcgtatg ccgttatctc aaacccggat
     7441 gctgaacatg tggtgatcta ttccttactt gttcatccgg atgcgcgtgg agatggactc
     7501 ggtttggata tgctgaaaag cctgatcgca aagcacactg ggaaaacctg gcacgtcccc
     7561 gccatactgc cagaggagtt gggaaaacta tacgaagaag ccggattcaa gaaggagaat
     7621 ctatcgcaat ggcaaatgaa actgaccctc atccccaaca cttctgtcaa gggagagagt
     7681 tagggtgaga gctttctgtc attcatttgt tttacgtctc tccagaatcg ggtatataat
     7741 catcgttctc aattggtcaa aagcacaaaa tgcaatcggc gctaaaacca ttctcttctg
     7801 aaagaaaaac gatcggtgtc ttcgcatccc aggtaggacg tgcatggggg gcagagttta
     7861 tggcaggcat tacacatgca gccgaggcga ataacgtcaa ccttgtgcat tttattggcg
     7921 ggaaattaat gtccttcacc gcgccgggtg agagtaagcc ttctttcgga ttatatgatc
     7981 tcgtcaaacc cgatcagttc gatggcttgt tgctaaccac agatgtatcc tatggcacca
     8041 gcgcgcatga tcttgaaaca tttacaaaag tttatggtac gctgccaatt gtcacgcaat
     8101 cagttgatat accgggcgcg tcgatgttcg tgcccgataa catcgagggg atgcgcgccg
     8161 tagtgcggca tctgatcgag gaccatgggt ataaacgtat cgcttttatt cggggtattg
     8221 aaggccaaat cgatgccgaa caacgttttc aatcctataa agacgaactt aaagcacatg
     8281 atctgcgttt tgatgaaaat tttgtagtca gaggcgatta cacacccgag agtggaaggg
     8341 cagcgatttt tacactgctg gatgaacgga agctccgctt tcaggcagtc gtcgcagcca
     8401 acgaccgcat ggcatttggt gctttggacg cgcttcagga acgtggcgtt cgcgtgccgg
     8461 acgatgtagc cgttactggt ttcgacgatt tacgcgaggc ccaatccacg ggtgtcccgc
     8521 tcacaaccgt acggcagtca ttttatacag cggggaaaca cgcccttgag actctgctgc
     8581 ggcgcatcaa tggcgaagca gttccggctg taaccatcac gcccacccaa ctgcttgtcc
     8641 gttggtcatg tggatgcttg cccgaaaatg tacgtcaggc tgccgtagcc ccacgtgacg
     8701 tggcgaagac agccaggctt gagaataagc gtgaggctgc gctgaaagcc ttactcgatt
     8761 cggctggcgt caccgaacaa gacccggcgc tgcttcaatt cagggatgct tttggacgcg
     8821 cctgggatgg ctttctcctg actctgaacg acaagtctac caacgatgaa tttctaaaga
     8881 ccatcaattc gatgatcgaa ctgatgcaaa agcagggact tccccctgcc ttgtggcata
     8941 acgtgatttc gatgatgcga aggtacgcgc tcggtggaat cacaagccat accaccatgc
     9001 tgaaggcaga aaatctcttc cagcagatac ggttgctggc tggtgaactt tcacaacgcg
     9061 cccaggcgta tcgcaggctg gtccttgaac aacaggaaaa tattctgcag gggttcagca
     9121 catccatggc acccgccatt agtctcaatg acattggagc cgccatctct gagcatttcc
     9181 cgtccatggg gattggacgc tggtatgtga tgttttacag tgacgtgacc gcaccacaat
     9241 ccatctcttc acctccacct gaaagctata gcctgctgct ccagtatgaa ggcgctaggt
     9301 ttgatattcc gacgagacgc gcctccatcg ggaccggtca gcttgtgccc cgcggtaaaa
     9361 ctccccagga ctatcgttat acagccgtca taatgcccct ttctctggca aagaatcgat
     9421 ttgggttcat gtgggtagaa atgggttcgc aggattggga aatctacgtc cgtattcgga
     9481 atctcgtcag cagtgcttta ttgcgcacga tgcttgtgca gcaaaaggaa caggcacagc
     9541 ataaaatcga acgactgctg gaagaagctc gtgaacgcgc cgctgaactg gcagttgcaa
     9601 aagagtgggc agaggtaact gcggcagaaa atgcaaaatt gtattccagc gagcagatgc
     9661 gccgtgaagc cgcggaggcg ctggcgaagt cctcgcgcct gctatccaca ttgggaactg
     9721 tgggcgaagt ccctcaacag atcgtcacac agttatcgca gctcataaaa aatgaacgct
     9781 ctgctttgtt cctggaggat gtaaatggta ttccgcgcct cctggcccat caaggtctgc
     9841 caaaagagtc gccagcaaaa gagttgagct acaaaataaa tggtacgaat atttaccata
     9901 ttgtcgcgaa acaatctgag tcaatggtga ttggcgatgt gaaaaatatg caaggctgga
     9961 gtcagtctct atggctgccc gaggatcgct cctggctggg cgtcccactg tattcaaaga
    10021 ataaagtggc gggcatgttg acgcttagtc gaaagaaccc agccgcgttc aaccaggatg
    10081 atgtactgct tgcgaccacg tttgcagtgc aggcctccat tgcactggat aacgcaagac
    10141 tctatgacga gttgacacgg tttaatcaga tgatggaacg aatggtcgag cagcgcgtcg
    10201 acgaactaaa cagtgcctat ggcacgcttg aaaaactcga taaaaacaaa agtgatttca
    10261 ttcaggtggc tgcccatgaa ttgcgcaccc cactaacagt gatcaagggc tatatgggca
    10321 tgcttaaatc ggcccaggcc attcaaggta accccacgct gatgcaggct atggacggcg
    10381 tcttgcaggg aacagaccgg cttcaccaga tcgtcaacag catgctggac gtcgcccgac
    10441 tcgagaacca ggttttatcc cctcaccttg aaatcgtgac tctgggtccc atcctgcgca
    10501 tgattcaaaa ggattatgcg gatgacctga ccgcgcgcaa tcttagactg gagattgacc
    10561 ccagggtcaa ttcgatccct gccattctgg ctgactctga actattgcaa aaggccattg
    10621 ataatgtagt tgtcaacgcc atcaagttca cacccgacgg aggctccatt aatatcagcg
    10681 cagatactgt taagatggac gggcgcagtg acatctgtga aattcgaatc caggataccg
    10741 gtataggtat cgaccctgcc aaccatgcca tcgtcttcga aaaactatat caattaggca
    10801 aggtggaatt gcactcttca ggcaagacaa aatttaaggg cggcggaccc ggtttaggac
    10861 tcgcaattgc cgccggtatc gtaaaggcac acaaagggaa aatctgggtc gaaagctccg
    10921 ggtatgacga agaaaaatta ccgggcagta cgttctttat ccaaattccg ctggcaaagt
    10981 aaataaataa ttgagccgag agttcaatcg cgtagtatcc ctgcgaactc tcggctcaat
    11041 ttgtgtttgg gcaatgctca attttcgttg cgaagcgcct tgctattctt atcgaaatat
    11101 tgggtcaact gtgcacgtat cggggcaagc agtccaatgt gtacaagcat aaagatcgaa
    11161 aaagcagaca ttgcaagaaa aaagaaaata tcattcaaga gataagcagg attggcaccc
    11221 gttgtgaaca cataacgaaa tacgatcaaa ccgagttgaa tcaagttaat aataagtaga
    11281 gggacagaga aaggacgcag atcatgatct tcgataatca aatgcaaccc aatcatcgct
    11341 cctaacccag caacgacggt cagtagatac gcccaaaatt ggagatctcc ataagcctgc
    11401 acatatagtc tttgcaggat cagcacataa agactgcaca caccgatcag gcatccccat
    11461 ataaaaatgt tgataataaa tggcagaacc aaattaccaa acacacggat ggcaaacatc
    11521 gcactaagcc atcccacagc ataagccagc ccaatgacga tgagctttgt agcaatccca
    11581 ttgatggaat ttaataaccc tacgctgaaa atatcgagga tcaaccgtgc accaccaagc
    11641 agtgcaaaca tcagcccacc gatgctcagc aggagcataa gaagactcaa cagacctctc
    11701 tgtgaaagtc ctctaaatgg cagcagtggt gatggacccc ttggcccggt gttgggtgaa
    11761 ttattcatac tacctcctgc ccacttgtga taaacagcac ctgacggaca tccttaaaag
    11821 aacgtcaggc gattattata cccataagac tgttttaacc cggaacgagt tccaaaattg
    11881 cggtttggtt cgttttctcc cctccccgcc aaccctctcg cttcataacc atacaagcct
    11941 gcttcttcag tacttgtgaa gttaacggtt attcctttga ataccgtgca tcctcggtcg
    12001 ttatcgtacc gcgagcccgc caggcaaatt tcgacgcgac acgattcggt ttacgctcca
    12061 gcacatcaga aatgatcctg cctagcaagg gcgtaaactt gaacgcatga ccgctatcgc
    12121 cagatgcaac catcaatccc ggtcgatccg ggtcatggtc gatccagaga tttccatccc
    12181 atgagtcaca gtaggggcag agccggttga aaagcacggg cgcagttgcc agcgcaggaa
    12241 atgttccttg cagaaaatct cggaattttt cttcctgatc ctctggcatc aggcgcggct
    12301 catccggatg gaagcgccaa cctgtaccat ggttcgcgat cttaaaacga tgatcttgaa
    12361 cggctgtgaa cccataccag cccgtattgg cgatatctgc cgcccatacc acgaagttcg
    12421 gtggagaaaa aatttccatt tcagggacct ggaagtggaa gaccggttga aagacggtcc
    12481 acaaaacgtc gctaagatgt ggaagcaggt gcggtgtcca cgcgcctgca gcgacgatga
    12541 catattccgc gtaatgcttt tcgctatcct cggtctggat tccgatgact cgcgagtcct
    12601 gatccagcag gtgcgagaac ttttctccaa cgcgaaatcg cacgccgatc cgctgcgcct
    12661 ccccatataa ccattcgagc acgcgtccgc tctccaccca gcccgcacga gggttgaaat
    12721 agccatcgct gtaaagttca gcgttccagg cagggaagcg gcgcttgagt tcctgcgagt
    12781 caagtcgctc tacgttgtgt ccgcgttttt tgagaagttg ataactctca tattcgaaac
    12841 tacccggctg catctcttca cgtttcataa tgacaaagcc ggtctcatgg tagagcggtt
    12901 ccttccaaag gcggttccat tcatcccata tgtcaaacgc ctgctccatc aggaccatgt
    12961 aatcttcatc agcaccgtaa tccatgcgca ggattttgct gatatcggtc gttgccgcca
    13021 atggatgggg aatcggaccc ggatcgaata catccacttc ccagccgcgc tgatgcaact
    13081 cgatggcggc ggaaattccg aaggaaccac cgccgaccac gatgacacgt tttctattcg
    13141 acattgatgc gaattattgt tgctgctgtt tcaagcgtat aaatacagcc cctctggcat
    13201 ccagaaaaat ttcatcagga gttttaacaa tacgcaggac aacatgctca cacatggaac
    13261 atcggagtac aacccctggt ccgtgggtga acgccaatag cgtacccatc tccgcctgat
    13321 taccacacga gaagcattcc ccgagcgcgg tggtcatatc cataccaaag atttcataaa
    13381 gcaggccggc tgctgcattt gcatccagca tcagttcgcg attgatgtct gttgtttctt
    13441 ccataaatca tctcccgtag taacgacttc acctgccctg gcgggcggtg ccagggaagt
    13501 cgttactaca attttatcct ccggtggggc cgaaacgttc ggttttgatc tgacttgatg
    13561 gaacaccgat cttgaccaat gaattggcgg ttgcttccac caacagcgtc ggtccacaga
    13621 taaacacttg aacagttttg cccaggggct gggcaatttc ttttagcatc tgctggtcga
    13681 tgcgacgtgc gtatcctttc caacccgcgg gttgggaacg cgtgagtgtg taaaggatct
    13741 gcaaagcgtt atttgccgcg cctaattttt ccaattcatt gtagtaaatg atatcttcaa
    13801 agtttcgtgc actgtaaagc agagagatcg gattccttgc acccgccgcg gcgtggtgac
    13861 ggatcataga catcaatggc accacacctg acccgccggc gatcaacaat aatggttccg
    13921 tcatggctgc ttcccagacg aaatatccac cgatcggacc acgcgcctcg aggcggtcgc
    13981 cgggaaccag cacatcatgc aggtaggctg aaacttcacc gtcgttgatg cgttccacgg
    14041 tgatatccac ttcgccttca cgctctggtt ctgaggcaat cgaatagctg cgttgagttt
    14101 ggtagccatc ctccgcagtc aagcggatat cgtagtgttg gcccgcccga tgacgcatcc
    14161 aggaggggag tgtgagcgta aacgacttta cgttcgcggt ttcaggcttg atgtctttaa
    14221 tagaagcgat ttgccagttg attgtgggtt tcatagtaat tcctatcgct cgcgctgagc
    14281 ggagtgacga gcgacagcga ggcatatagt cgaagcgctt tttgcaggca ttgcccttcg
    14341 actgcgcttt gctccgctca gggcgatact aatcattcca ataccgctgt tccttccacg
    14401 ggtcgccgcg gttgtgataa ccggccgatt cccagaagcc cggtttatct ttttctgtca
    14461 gataaatgcc gcgtacccat ttggcgctct tccaaaaata cagatgcggc acaaccatgc
    14521 gaacgggtcc gccatgttcg ggtctgagag gcttatcgtc atattgccag gcgatgaagg
    14581 attgaccatt gatgatgtct gtgagaggca tattcgtggt gtatccgcca tcactataag
    14641 ccgtgacaaa cgcagctttg cggtcaagtt ccacgtgctc caacagtgta tccaggctga
    14701 cgccctccca cttcatatcc agcttggtcc acttggtgac gcaggaaata tctacaacat
    14761 atttctgcat cggaagtttc aggaattctt cccacgtcca tgaaactttc tccttgacca
    14821 gtccctcaat gctgaaagtc caccggtcga gcggtgtgta tggagtcgga ccggccgaaa
    14881 gaacaggaaa atcgtttgta agatattggc caggcgggat gcgatctttt ttctcgcctc
    14941 ccctccgttt gccaacaaaa ccacgtgaga caaatcgttc ttctgtcatg gcaactcctc
    15001 tttgtttgaa agatattcac ggatacccaa cgacattata ttcccgattc gtgtgattgt
    15061 attatttgtg tttttcaagc aatcggacaa acatctctac aatgcgcggg tcgaaatgtt
    15121 tacccgactg ttcatggata tactgcaggg ccttttctct tgtccatgcg tcgcgatacg
    15181 agcggtcaga cattaaaaca tcccacacat ccgccactgc aaagatgcgc gcaggcaatg
    15241 gaatatgatc tccctgcaag ccatgcggat accccgtccc atcccatttt tcatgatgaa
    15301 aaaatgggat atccattgca ggtgacagat aggaaatccc agaaaaataa tcgcgagcat
    15361 agtccggatg tttgcggatc aggccccatt cttcctccgt caacgcgcca ggtttatgaa
    15421 ggatcgcatc cggaattccc attttgccga tatcgtgcag aaggacaccg cgtcgtacct
    15481 ggatgaggtc tggccctcga atcccgagct ttcgcgccag tgacagggtc aggttcaaga
    15541 ctcgctgcga atgacccatc gactctgaat tacggagttc gagcgcattt gcccagcctt
    15601 gaatcgtttc atcgtaggcg tgcactgctg cacttatact tttattgaga atcctggata
    15661 cgatataagc cccaatcgca attatgatga gagccacaag tgggaaaccg ccaaaatttg
    15721 acgcttgtgt ggactgcgcc ggaagccacc aaaaactgat gatggaagca cctgtaaaaa
    15781 gaaaggcggc ccagggttga atgacgaagc tggcaatcac aacaagccag gcgatcacaa
    15841 agaatctcaa aggaaagtcc aaagtgccat acgatacaag gatcaccgta aacaggaaca
    15901 aacaaaatat ccatcctacc agcggaacgt atcccttttg attcatccac aacaaaatca
    15961 gaaatacagt gattgttatc ccagtggaca ccggattaat cccttccagc tcaatcaggg
    16021 tcgagggtag ccatcccatt cgatcgaaga tggttaatcc gaaaaggata accatagagg
    16081 tcaccaacag aacgcgcaaa agcttttgcc gataatccaa cagaatatat tttgaataat
    16141 catccaacac aaaattggga tttgtttttg tcatttcacg tcgcaagact gagcatggga
    16201 ataataaaca gccctacaag tattgcaggg ctgtcgaaaa accaattcta gactctaaat
    16261 atggatgggt tgcccttctg cgccatgcgc agcttccatt atcacttcgc tcaacgatgg
    16321 atgtgcatgg acatttcgtg cgatctcatg aggagtgagt tccatcaaat gcgcaagggt
    16381 cagttccggg agcagttcgg tcacttcagg gccgatcata tgagcgccca aaatctcacc
    16441 atacttttca tccacaatga tcttgaccca acccgcgtaa tcacccagcc caagtgcctt
    16501 tccatttgcc tggaacggga accgaccgac cttgattcca tacccgcgtt cctttgcctg
    16561 tgcttccgta tatccgaatg aagcaatttg cggatgggaa taggtagcgc gcggcatcat
    16621 gtcataatca aggacaattg tttccgcacc ggcaatattt tctgcacaga caatgcccat
    16681 ggcggaacca acgtgtgcca gcatgagttt cccggtcaca tcgccaatcg catagatatt
    16741 cggtacgctg gtctgcatct tttcattgat ttcaaccaga ccgcgctcgt tgattttgac
    16801 accagcttct tccagaccga atcctttgga gttgggacgg aacccaattg cgacgagtgc
    16861 ctgctcggct tcgagggtgg tctccttgcc ctctgcagaa actttcacct tcacgcctgt
    16921 ttttgtggtt tccaccgatt caactttatt tccaaccaaa catttgatgc cgcgtttttc
    16981 gaattccttc ttaagatcct ttgagatttc ttcatcctca agcggaacca gtcgcggcag
    17041 catttcaaca atcgtgacat ccactccgta cgaattccag acggttgaaa attcaacgcc
    17101 gatcgctccg gacccgatta cgatcacact ctttgggagt ttttcctgca aaatggcttc
    17161 ctgatacgtc actacctttt cgccatccac tttccaggca ccagggactg cggcagtcgc
    17221 accggttgcg atgatgatat tctttgcttt tatttcagta gctttgccat ccttatcggt
    17281 gacagtcaga gtgtctttgc ttttgagttt tgcctcaccc ataaacaccg cgatgttatt
    17341 ttttcgcatt aaaaagccca cacctttgac caggcgatcc gaattttgcc gcgagcgttt
    17401 gaccgccacc ggataatcca gtttcaggtt atcgaatgaa aagccaaaat ccttgccgcg
    17461 ttcgcgcagg gtgtgagcga tctctgcatt tttcaataac gacttggaag gaatgcaacc
    17521 cacattcaaa cacaccccac cgagccattg cttatcaaca attgcaactt tctgtttgag
    17581 ctgggcagcc cgtatcgcgg cgacgtagcc tgcaggtccc gcgccaatga caacgacatc
    17641 aaaattttct gccatgaaaa aatctcctta agttatcgag aggttcatct cgaaatttta
    17701 ctggaacacc ttattttacc cgttttcgca catgatacac ttgccagcaa tgacgatgat
    17761 ggaaaccttg cggagccgca aattgatcat ccccatgctg gtcgtcttcg tcgcgctgct
    17821 tgtccaaact tcatgggccg aagaggatgc ttttattacg ctgcgcacct ccgacaatct
    17881 gatccacgga tatggaccac gctggaatgt ggctgaacgg gtacagacct tcaccaatcc
    17941 attttggatg tttctgctgg cagggactca tgccataatc aagaatcccc acatcactct
    18001 aatcttactc tcgatctttg tctcagtgct ggccatgttg atcatgcttg tctatctccc
    18061 tcaaacgaac tttggcgtag tactggcatt ttctatttta gttttatcca aggccttcgt
    18121 ggatttttcc acatcaggtc tcgaaaaccc cgcctcgcat ttgttgatcg tctgctttgc
    18181 ggctttctat ctgcgttctg agaagccttt gtcggaacgg aatctcttct tcgtaagctt
    18241 gtttgccggg ttggcagcct tcaatcgcct ggacttattc ttattttgtc ttccggctct
    18301 ggttgccaca ttgtttgcac agttcaacag gcgaaacatc ctgatcatgt tggccgggct
    18361 ttcgccggta ctcgtatggg aaatcttcgc tgtgatttat tatggatttc cggttcccaa
    18421 cacgtattat gccaaactcc atacaggcgt gccgttcaac gaatcgataa cacaaggcat
    18481 tttatatttc atcaactctt ttggctggga cccaatcaca cttacgattg tgttctcggc
    18541 aatcgggcta cctctcgtaa gcaggtacca cgagaggatt ctgctcgccc tggggatagt
    18601 cttatatctt ggttatattc tctacattgg cggcgatttc atgagcggac gcttcttctc
    18661 tacacccctt atgataagcg ttgccttgct tgttcatcgc gtcgaagatg ctacaacgtt
    18721 ggaaaaatat gcctggatca gcctcgtact tttgctcgga ttaatccttg ctccactgaa
    18781 atcattcaac aatccgattg aaagcgatgt catttccttc gatagcaccg cagggatcgc
    18841 cgatgagcgt ttcggatact atcgctattc gaatctcctg cttttcagca gagatcagga
    18901 ctttcagctc tatgaatttg cgcagtatgg catttatcta cggcgcgaaa aaataccgct
    18961 ggccacgatt cccgctatcg gtatggttgg gtattacgct ggtccgggga cgtacatcat
    19021 tgataagctc ggactcggag acccacttat ggcccggctc aagcggactg acccgaaaaa
    19081 ctggcagatt gctcacatcg agcgtaatgt cccagacgga tatggcgatt cgattgaaaa
    19141 taatgaaaac aggatcaacg atccggccct ggctgagtat tatgataagc tgctactcat
    19201 tacccgggga gatatctggt cagcacaacg ctgggaagcg atctggaaaa tgaatacggg
    19261 acagtacgat tatttgcttg aacggtacgc aaaatcgcag aaatagtctt tgatttcaac
    19321 caagattttg cttcagaaac tcgaaggtac gattccacgc caaatcagag gcggcggaat
    19381 catattccgg acgatcctct tcgacgaacc agtgagcaac agccgggtag atgtgaaacg
    19441 tggagtccac acttgcatcc ttcaggcttt gctccagcca acgcacacct tccataggtt
    19501 cccactcatc gttgtcgctg aaatgaccca ggatcttcga tttgactttg ctgaactccg
    19561 tctctccaat cccgtaaaac aaaacggtgg cggcaatttt atcgggatca taacccgcga
    19621 cgacgagtga ccaggcggct cccatagaga agccaaccac gccgattttc tcgcccccag
    19681 agtgggccag cagataatct tttgcggtca tgacaatatc accgatcacc tgattgtcac
    19741 tcttttccat caactccttc gccgaatcga tggtttctgc aatttgtcca ttgcgcatgt
    19801 caggggccag cgcgacgaat ccctgctcgg caaggcggtc acatatctgt ttgaagaatg
    19861 gtttgagtcc ccaccaggca tgaaggacta aaacacccgg cccgccactt tctggcgcag
    19921 caaggtaggc attgacgggt tgaccgtcca catctaattt gatttcggat tttcgaacag
    19981 acatgacgca cgctcctttt tgcttgatta tatcagaaca tacgttctaa tttacaacca
    20041 ccgtctcgcg cgggacttgt agctggtgta tgcttcccca aactttttct ccagatacgc
    20101 ctcttctggt tggatgacca gcgtgttcat caagaatatg aacaagggac tgaggatcaa
    20161 tccccaatag cttccaaacg caaacggaaa accaattaat aaacagacaa atccaaggta
    20221 gattgggttg cgagaaaaac gataaacgcc atctttgaca attccactca ccgagccatg
    20281 cgggtcaagc gtagtacgcg ctcgtatgaa tcgaatcact gcactgaacg ccagcccaag
    20341 accaacaaca accagtagat aaccaaccca tatcagaggg tctggaggca caaatggaaa
    20401 tagccgattg agaagataca cggcacccat gtggaggagc aataaaacag gcgggtgtat
    20461 tttcacgctt gcatggtcag gatgttcttt atctgttgcc attctaactc cacccttcta
    20521 aagtttcctt caccttcatc aaaaatgcat ctgctctcgc gccatcaagg atacgatgat
    20581 caaatacaaa cgagagatag accatcgggc gaatggcaat cgcatcatcg ataacgacta
    20641 ctcgcttttg catcgcaccg atgcccagga tgccacactg cggctggttg atgacgggat
    20701 aggcgaacag tgaaccgcta attccatgat tcgtcagagt aaacgtcccg cctttgacat
    20761 cgtctggttg cagcttcttc gcgcgggcac gattcgccag atcattgacc gcgcgcgcca
    20821 ttgccaacag ggataaatta tccgcatcct tgatcaccgg gacgatcaat ccatcctcgc
    20881 caagcgaagt tgccattcca aggttgactg ccttatgaac gagcactccc tcctctgtcc
    20941 atgaagagtt gacaatgggg taattcttca atcccgctac aatcgcggtc ataaaataag
    21001 cggtgtaggt gagattgatt ccgtctcgtg caaagatctc tttgtttgct gagcgatgtt
    21061 tgccgacgcg gctcaggtct gcttccatca cggtcagtac atggggagag gtatgtttgc
    21121 tcatcaccat atgctcagcg atttgttttc gtatgacggt gtgtttgatc aattgatcgc
    21181 cggcaactgg agtaatcgtg ggagcagaag gcgaagaggg ctgagaaact gaaaaggttg
    21241 atttgccagt ttctacaaag ttcaagacgt cgttcttggt aatgcgtcca ttcaacccgg
    21301 tacccgtcac gttctgtaaa ttcaccccat gctccgctgc gatttttgcc acaacaggag
    21361 aaatgaaacc gatatctttg ttgtccgttg gtgtttgggg gttgtcagtg gaagcctgat
    21421 cgtgaacggt taccggttga gaactgacgg gtaatggttt ttcgctgcca accgatgact
    21481 gtccactgtc aaccacttct ccaggcttgc caataaatgc gagcaccgtc ccaactttcg
    21541 ctggtgcgcc ctcctccgcc attattttca acacagtccc tgatgctggc gcagggattt
    21601 cagtatccac tttgtcagtg tttacctcaa gaagcggctc cagctcattg actgagtcac
    21661 cttcctgttt cagccacttt attacagtaa cctcttcaac accctcaccc agcaatggga
    21721 cgaaaacttt tgttgccata atcactccgg ttgatggttg gtagttgtca gttgatcgtt
    21781 taacgagcag gcaagctatt tatcaatgcg tacattttct tgcgcaggcc ttgaatctgt
    21841 tcatctatca cgtcaacatc ttctttgctc agatacccaa attccttggc taatattagt
    21901 tgggtgtcta cttcggctaa tgaacctagt gaaaaatata aaaaacgacg gaaatcacct
    21961 ggagctttac gagcctgacc ctctgcaata tttgacggca ctgagacaac ggcgcgacga
    22021 atttgatctg caagggcata tgtctcttga cgtggaaatt tctcggttag cttatacacc
    22081 gcttttgcga gtttaatgcc ttcctgccag acaatcaact cacgataatt ccgggcagct
    22141 actttattgt tatccaaggc ggtcaaccac caacttataa ccatcaacga agcggcggat
    22201 acgccgccgg atctacttca ttcatcatct catacacagt atcaaaaatc gtttcggcat
    22261 ttggcttgga ccagtaatca ccatcactcc cgtaggcggg gcgatgtgct ttgccactta
    22321 gcgtgcgagc gggagaatcg agatggaagt aaccaccttg cttctcaatc acttcctgca
    22381 gcatgtaggc ggaggcaccg ccaggcacat cttcatctac aaacaaaatg cggttggtct
    22441 tcttaagcga ctcaacgatc tttccgtgaa tatcaaatgg caataaggac tgcacatcga
    22501 tcacttcaac ttcaatgccc accctggata atttttctgc tgcatccagc gcaatacgac
    22561 agcacgcgcc ataggtgacc agcgtgagat ccgcatttgc gccttcgcga atcacctctg
    22621 gcacacccag cgggaccgta aattcaccga tattatctgg aaccctttcc tttgcgcggt
    22681 aaccattcaa tacttcaacc accacagcgg gttcgtcagc tttcagtagc gtattataaa
    22741 aaccagccgc gcgcgtcata tcccgtggta ccaacacgtt gattccgcgc acaagatgga
    22801 tcagccctgc cattaacgag cccgaatgcc agatgccttc caaacgatgc ccacgggtgc
    22861 gcacaattac aggggcagac tgtccgccta ccgtccgcca gtgtagattt gccaaatcgt
    22921 cagacatgat ctgtaacgcg taaaggacgt aatccaaata ttgaatttcg caaataggtc
    22981 gtagaccacg catcgccatg ccaatcgcct gacccaaaat ggtcgcttcg cgaattccag
    23041 tatcgctcac tcgcaatgcg ccatattttt cctgcatccc tttgaaaccc tgattcacat
    23101 ctcccagaaa gcccacatct tcaccaaacg cgatcacgcg cgggtcacgc gacaaaattg
    23161 catcaaaagc catattcatt acttcaaaac ccatcattgt cggcgatgaa cttgaataaa
    23221 ccggcttcac ttccttcaca gtgaccgctc tgccgctata tagatgcgaa ccgtaacgtt
    23281 cttcattgac ggcgtcctgt tcgttcttcc attcgactaa aacctgcttg gatggatgca
    23341 gttcatcgcg caggattctc aaggcttcat gggcagcgga gtggacgtcc cgcctcagtg
    23401 gtgcagggag ggcagagagc ctctctttga tgtcgttcaa ctctgacgca tgttttgaac
    23461 tccctgcgat ctcatcgaac atgtccatga cctgactgcg ctcctcgatg atcggcgcga
    23521 gataggcgtc ccaggcaagt ttgcggatgc cctctacagt gtcgtggtcc tctttttcaa
    23581 cggccaacag ctcaggctcc ttaatgaccc gattctccag catccactcc cgcatcttca
    23641 ggagtccatc aaactcctct tcaaatctca aacgtgcagg cgatttatat ctttcgtggc
    23701 tgccggatgt ggaatgtcct tgaggttgag tcacttcggt cacatgcacc aacgcaggga
    23761 tgtgatgctc ccgcgccgtt tcagtcgcgc gcgcgtaggt ttgcaggagc gcgggatagt
    23821 cccaggcttt gacgcaataa acatcgaagc cttcatttga atctggcgca cgctggaatc
    23881 ctttgagcag ctcgccgatg ttccctttta ccatttgatg ttcattgggc accgagatgc
    23941 catagccatc atcatagata gaaatcactg ccggagcatg aagcacacca atggcattga
    24001 tcgactccca gaacatgcct tcggcggagg aagcattacc aatgctcacg aaagcgactt
    24061 cgttaccatt tttggagaat tgcgtgaatt gttttaactc ttcgaccccg c
//
LOCUS       DASTJP010000072         7176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1501686, whole genome shotgun sequence.
ACCESSION   DASTJP010000072 DASTJP010000000
VERSION     DASTJP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501686"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..2604
                     /gene="priA"
                     /locus_tag="VFQ23_02245"
     CDS             136..2604
                     /gene="priA"
                     /locus_tag="VFQ23_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET9905423.1"
                     /translation="MTTYIQIVVNVPAVVGIFDYAIPENLLGKIGVGHLVVVPFGNQT
                     VQGVVFRFIDQPSVRDVKEVIELVDPEPVVTQAQITLAEEMAEHTLSPLASIVSLFLP
                     SGLSQQVDTLYEIRDLALQTQKSADPQSRSSNTQKLRTEDVLLNLLRMRGPLRGRQID
                     THFSKVDWRRTAQFLIRKGVLSSRSVLPPSRAKPKFIKTAQLAISPEIAESAMTDLGK
                     TNATQARREKALKFLMRVAEAVNVSWVYAESGCNLADLQELEERELIVLRETEIWRDP
                     LQRVESKDLESSRVGKLTNEQQAAWQEIEKTFREIGEGKSAKPFLLDGVTGSGKTELY
                     IRAAQEAIRRGQQTIVLVPEIALTPQTVRRFLNHFPGQVGLVHSKLSEGERYDTWRRA
                     RAGLLKVIIGARSALFAPLPNVGLIVADECHDSSYFQSEPPFYHAITAAQIYARLAGA
                     VCILGSATPSVSQRHDSEIQKVKRLELPHRIVQTGLPPVHIVDMREELKSSHRGIFSR
                     FLLAELEATLKRGEQAILFLNRRGTATYVFCRDCGFVLRCPNCETPLTFHLEDKERLL
                     CHQCGYERGKPKSCPDCGGKQIREYGLGSEKVETEVNALFPKARTLRWDWDTTRQKDA
                     HEMILTHFAAHRADVLVGTQMLAKGLDLPLVTLVGIVLADVGLHLPDPFAGERVFQVL
                     TQVAGRAGRSERSGKVVLQTFDPTNQVIQSAARHDVSEFYQYELGQRKRLGYPPFARL
                     VRLEYRHSDAAKAEEEAHRVAKKLSTVIRSQTTMIGPVPCFFAKVGGYYRWQIVLRGS
                     NPKDDLRDKKLEGWRVEVDPVSLL"
     gene            2760..3662
                     /locus_tag="VFQ23_02250"
     CDS             2760..3662
                     /locus_tag="VFQ23_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905424.1"
                     /translation="MESFSPGWNFLKQAWSMAFKDKDLLKPSLYALIAGTLVSIIGVI
                     PIIGAFFLFGNSQVGNIILFILGAIMIFVQFVVSYIFSGMTVYLIYGYLSEGDGRMDK
                     AWRIVQRDFIDILTLAAVSTAVNLVRNAASRNRGGIAASLTRGATNLLQVLWTEASYL
                     ILPAMVIDDLNLKDGMQRILKITRENLLLIGISTVGVRWVTGLISAALGLGGFFLAFA
                     IGGGAAYLTGGVNAISIIGVAIGALIFFTFILAASIISSYTSTAYHTCLYIWARDVEK
                     ARLTVDAPVPVQVSAPAPLAAVLG"
     gene            3707..4216
                     /locus_tag="VFQ23_02255"
     CDS             3707..4216
                     /locus_tag="VFQ23_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET9905425.1"
                     /translation="MQPKPQRRREMITWDEVDRLIDHLVPQFKREYTAMVIITRGGII
                     PGGLLADAMDITHILTAAVDFPAQFESRKSTLMAWPEFIQFPSEDKLRGRPTLIVDDV
                     WGGGRTITAVKNRVSAAGGFPETCVLHFNPYRNLFGSVRPDYYAAVTDAFIIYPWEVD
                     RGPDQVLLG"
     gene            complement(4306..4773)
                     /locus_tag="VFQ23_02260"
     CDS             complement(4306..4773)
                     /locus_tag="VFQ23_02260"
                     /inference="COORDINATES: protein motif:HMM:NF040341.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HET9905426.1"
                     /translation="MKKLILLIAVFALALSACASATASANQNQSELDKNQQTWQDAGI
                     SHYRFNVSISCFCVFSQDMPLIIEVKDGQVVSMEFASGNEIDEANREYFARFETIDRL
                     FEQLQKDIGGEADEVVVTYNTTYGFPEEVKIDYVLEATDDELWLTVSDFEALQ"
     gene            complement(4828..5274)
                     /locus_tag="VFQ23_02265"
     CDS             complement(4828..5274)
                     /locus_tag="VFQ23_02265"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HET9905427.1"
                     /translation="MKRILMIAFAVVFFLSGCSAEEGIVVHNAWVRPTTRGENGAVYF
                     VIQNNSGATAELIAASSNVAESVEMHESSIAEGDVMRMQQVFSVSMEHRSEIIFEPGG
                     LHVMLVNVNRDLELGEYVELILHFKNHEDIPVTVSVSEFAPDEHDH"
     gene            complement(5271..6488)
                     /locus_tag="VFQ23_02270"
     CDS             complement(5271..6488)
                     /locus_tag="VFQ23_02270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9905428.1"
                     /translation="MKKPIAAPPYESPNFLPRFMIYFLLSSAFFISGIVVIIWLHNQY
                     HLDIVVEPAPPPSPAPLISVCIPARNEERNIRACVEALLAQDYPNLEVIVLDDRSSDA
                     TPFVLAEIAALDSRLHPISGSDLPKGWAGKPHALFQAAAHARGEWLCFVDADTVVHPN
                     TLSSCYSKAIETQADMFTIMTFQVLDSFWEKVVMPLVLTALSVGFSPRKINDPNRKDA
                     IANGQFILIKRATYLALGGHESIRNSIVEDKALAQKVKWNGYRLIVANGMRIVRTRMY
                     TSLPEMWEGWTKNIYLGLHDQPSLLLLGVFGAIVLLVSAFFLPIWPLIGSLWYSQGGG
                     WMAGGVIIQSLLIWALLIRERVKVNQQLEISPWYSLTTSLGAGVFAAMMLASAWKVLS
                     GRGVTWKGRVYSS"
     gene            complement(6448..>7176)
                     /locus_tag="VFQ23_02275"
     CDS             complement(6448..>7176)
                     /locus_tag="VFQ23_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HET9905429.1"
                     /translation="TAKKNLESAEAEAPNLDSAERELYRLREEENKKRSELGGAQQRV
                     DVLTVQRSRKLQFETDREELVKHIAQHKTLERAFGKDGVPALLIEQALPQIETKANDL
                     LDRLSDGQMSIRFVTQAEYKDKKRDDLKETLDIQISDAAGIRAYEMYSGGEAFRVNFA
                     VRLALSEILAQRKGARLQTLVIDEGFGSQDTRGRQRLLEAINLVKNDFAKILVITHLD
                     ELKDAFPSRIEVEKTDRGSSVRVT"
BASE COUNT         1737 a         1759 c         1832 g         1848 t
ORIGIN      
        1 tgtggggtat ggcgcggccg tgatattgaa agccagataa tcgatttcct attgatgcaa
       61 taagcatcga acagttttta ttgcttattt gtcttgctta cgtgttgggg ttttcaagat
      121 acaatagtga ccctcatgac cacgtatatt cagatcgttg tcaatgtccc tgctgtagtt
      181 gggatatttg attatgccat tccagaaaat ctcctgggga aaattggagt ggggcacctt
      241 gtcgttgtcc cgtttggaaa tcaaacagtt cagggtgtcg tatttcgttt cattgaccag
      301 ccttcggttc gggatgttaa agaagttatc gaactggttg atcctgaacc tgttgtcaca
      361 caggcgcaga tcacgctggc agaggaaatg gcagagcata cactttcacc gttggcatcc
      421 attgtcagtt tgttcctgcc gtcgggacta agccaacaag tagatacatt gtatgagatt
      481 cgggatctag cgttgcagac tcagaaatcg gcagacccgc agtcaagaag ttccaataca
      541 caaaaactcc gcaccgaaga tgtgctattg aacttactgc gcatgcgagg tccactgcgt
      601 ggccgccaga tcgatacaca tttttcgaag gttgactggc gcaggacagc acaatttctc
      661 attcgaaaag gtgtgctttc gtcgcggtct gttttaccgc cctcacgtgc gaaaccgaaa
      721 tttatcaaga ccgcgcagtt ggctatttca cctgagattg cagagtctgc gatgactgat
      781 ttagggaaga ccaatgcaac ccaggcgcgc cgtgagaaag ccttgaaatt tctgatgcgt
      841 gtagcagagg cggtcaacgt ttcatgggtc tatgccgaaa gtggatgcaa cctcgccgac
      901 ctgcaggagc tggaagaacg cgaactgatt gttttgcgag aaactgagat ctggcgcgat
      961 cccttgcaaa gagttgagag caaagattta gagagtagcc gagttggtaa gctgacaaat
     1021 gaacaacagg ctgcgtggca agagatcgaa aaaacatttc gcgaaattgg ggagggaaaa
     1081 tcagccaagc catttttgct ggatggtgta acaggctcag gtaagaccga gctttacatc
     1141 cgtgctgcgc aggaagcgat tcggcgcgga caacaaacta ttgtcctggt cccggaaatc
     1201 gccttaacgc ctcaaacagt ccgtcgcttt ttgaatcatt tcccggggca ggttgggttg
     1261 gtgcattcga agctatcgga aggtgagcga tacgacacct ggcgacgtgc acgcgcaggt
     1321 ctgttgaaag tcatcatagg ggcgcgcagt gctctgttcg cgccgctacc aaatgtcggt
     1381 ttgatcgtcg cagatgaatg tcatgactcc tcctattttc aatcggagcc gccgttttat
     1441 catgcgatca cagccgctca aatttatgca cgactggccg gcgcggtctg catccttggc
     1501 agcgcgactc cctctgtttc acaacggcat gattcagaga tacagaaggt gaagcggctt
     1561 gaactgccgc accgaatcgt ccagaccggg ttgcctcccg ttcatatcgt ggatatgcgg
     1621 gaagagctga agtctagtca ccgcgggatc ttcagccgat ttctcctggc tgaacttgaa
     1681 gccacactca aacgcggtga acaggcgatt ctatttctta accgccgcgg caccgccacc
     1741 tatgtgtttt gtcgcgattg tggctttgtg ttgcgctgcc cgaattgtga aacaccactg
     1801 acatttcatc tcgaagataa agaacgactg ctttgccatc aatgcggcta tgaacgcggc
     1861 aagccgaagt cctgcccgga ttgcggtgga aaacaaatcc gagagtatgg tttgggcagc
     1921 gaaaaagtgg aaacggaagt gaatgcctta ttccctaagg cgcgcacgct tcgctgggat
     1981 tgggatacaa cgcgtcaaaa agatgctcac gaaatgatcc tcacacattt cgccgcgcac
     2041 cgcgcggatg tgttagtcgg cacgcaaatg ctggcaaagg gcctggatct tccactagtg
     2101 acgttagttg gaattgtgct tgccgatgtt ggtttacatt tgcccgatcc gttcgcgggc
     2161 gaacgcgtct ttcaggtgct cacccaggtg gcgggccgcg cgggtcgaag tgaacgcagt
     2221 gggaaggtcg tgttgcagac gttcgatccg acgaatcaag tgatccaatc tgcagcgcgg
     2281 catgatgtga gtgagtttta tcaatacgag ctgggtcaac ggaaacgttt gggttacccg
     2341 ccgttcgcgc gtttggtgcg tttggaatat cgtcattctg atgcggcaaa agcggaagaa
     2401 gaagcacatc gagttgcaaa aaagctttcc acggtgatcc gctcgcagac cacaatgatt
     2461 ggtcccgttc cctgtttctt tgctaaggtt ggcggctatt atcgctggca gatcgttctg
     2521 cgtgggtcga atccaaaaga tgatttgcgc gacaaaaaac tggaaggctg gcgggttgag
     2581 gtggaccctg taagtttgct ataattattt tttagccacg ttggtcgagt agccttgagc
     2641 gatccttgcg aagggcatat cgagaccacc tactaagaaa agtattatca tagcatgttg
     2701 gtctcgatac gagccttgct ctactcgacc aacggatttt tcatcgagga gaattcatca
     2761 tggaatcatt ctcgcccggc tggaactttc taaaacaagc ctggagcatg gcgtttaaag
     2821 ataaagatct acttaagcca tccctatatg ctctaatcgc cgggacactc gtctccatca
     2881 tcggcgtgat cccgatcatt ggagcgtttt tcctttttgg gaactcacag gttggtaata
     2941 tcattctgtt tattctcggt gcgatcatga tctttgtgca attcgtggtc agttatattt
     3001 tttccgggat gaccgtctat ttgatctatg gctatctttc cgaaggcgat gggcgaatgg
     3061 ataaggcctg gagaatcgtt cagagggatt tcattgatat tcttaccctt gctgcagtgt
     3121 ctactgcagt gaatttggtc cgcaatgcag cgagtcgcaa tcggggcggg attgctgcga
     3181 gcctgacacg tggcgcaact aatctgttgc aggtattatg gacagaagca tcctatctca
     3241 tcctgcctgc catggtgatc gatgacctga atctcaaaga tggcatgcaa aggattttga
     3301 aaattaccag agagaatttg cttttgatcg gtatcagtac agtcggtgtc cgctgggtga
     3361 caggtttgat cagcgcggcg cttggacttg gcggattttt tcttgccttt gcaattggtg
     3421 gtggcgcggc ctatttaacc ggcggtgtca atgccatttc gatcattggg gttgcaattg
     3481 gcgcgttgat cttcttcaca tttattctgg ctgcgagtat cattagctct tatacctcga
     3541 ccgcctatca tacttgtctt tatatttggg cgcgtgacgt agaaaaagcg cgactgaccg
     3601 ttgatgcacc tgttccggtg caggtctctg cgccagcacc gttggcagca gttttagggt
     3661 aagatacata cgaaagaaac tcgtctgatt ccaaaagttg aatcctatgc aaccgaaacc
     3721 acaacgacga cgtgaaatga tcacctggga cgaagtagat cgcttgatcg atcaccttgt
     3781 tccacagttc aaacgcgaat ataccgcaat ggtcatcatt acgcgcggcg gaatcatccc
     3841 tggcggacta ctcgccgatg ctatggatat cactcatatc ctcaccgccg cagtggattt
     3901 cccggcacaa ttcgaaagcc gtaaatctac cctaatggcg tggcctgagt tcattcaatt
     3961 tccatcggaa gacaaattgc gtggccgccc aactttgatt gtggatgatg tgtggggggg
     4021 tgggcggacc atcacggcag tgaagaaccg cgtgtcggca gccggcggtt tcccggaaac
     4081 ctgtgtgctg cacttcaacc cctaccgcaa cctgtttggt agtgtgcgcc ccgattacta
     4141 cgccgccgtt accgatgcgt tcattatcta tccgtgggaa gtggatcgag gtccagacca
     4201 ggtattgctc ggttgaactg aatagcaaac aaaaacaggc gacttatgca agtcgcctgt
     4261 tttgttaccc taacttttct tgaagtgaaa tgggtcttgg gtatattact gcagtgcttc
     4321 aaaatctgaa actgttaacc agagttcgtc atctgtagct tccaggacgt agtcaatctt
     4381 gacttcctcg gggaagccat aggtcgtgtt gtaggttaca acgacttcat ctgcttctcc
     4441 accgatatcc ttttgaagct gttcgaagag gcggtcaatg gtctcaaagc gggcgaagta
     4501 ttcacgattg gcttcgtcga tttcgttacc gcttgcaaat tccatcgaaa ccacctgacc
     4561 atccttgact tcgatgatca acggcatatc ctggctaaat acacagaagc atgagattga
     4621 aacattaaat cgatagtgcg aaatacccgc gtcctgccag gtttgctgat tcttgtccag
     4681 ttcactttga ttttggttag cgctggcagt tgcactggca catgcagaca gtgcgagtgc
     4741 aaatacagcg atgagtaaga ttagtttctt cattttatct ccttgcacga tggacgccgc
     4801 attcttccca aaagttcccc gccaccatca atgatcatgt tcatccggtg caaattctga
     4861 tacggaaacc gttactggaa tgtcctcgtg gtttttgaag tgcagaatta attcaacgta
     4921 ttctcccagt tcaaggtcgc ggttgacatt cactaacatc acatgcaatc cgcctggctc
     4981 aaatattatt tctgagcggt gctccatgga cacagaaaac acttgttgca ttcgcatgac
     5041 atcgccttct gcaatcgagc tctcatgcat ttccacagat tccgcgacat tcgatgatgc
     5101 cgcgatcagt tcagctgtag ctccagaatt gttttgaata acaaaataga ctgcgccatt
     5161 ttcgcccctg gttgttggac gcacccacgc gttatggacg acaattccct cttcggctga
     5221 acaaccagag aggaaaaaaa caactgcgaa tgcaatcatc aaaattcgtt tcacgacgaa
     5281 tacactcttc ctttccaggt gacacctctg cccgaaagaa ctttccacgc agatgcgagc
     5341 atcatggctg caaagactcc ggcgccaagc gaagtcgtaa gtgaatacca cggggatatt
     5401 tccaattgtt gattaacctt tacacgctcc cgaattaaca gagcccatat caggagcgat
     5461 tggataatta ctccgccggc catccagcca ccgccttgac tgtaccaaag acttccaatc
     5521 aagggccaaa tggggaggaa aaaggcgctg acaagcagga caattgcgcc aaacactccg
     5581 agcagtaaca gagatggctg atcatgcaga cccagataaa tgtttttcgt ccagccctcc
     5641 cacatttcag gcaaggatgt atacatgcgt gttctcacga tccgcattcc attagctacg
     5701 atcaggcgat agccattcca tttgaccttt tgggcgaggg ctttatcctc gacaattgag
     5761 tttcttatac tctcgtgtcc accaagcgcc aggtaggtcg cgcgtttaat gagaataaac
     5821 tgcccgttgg cgatggcatc tttccgattg gggtcattga tcttgcgagg cgagaagccg
     5881 accgagaggg ccgtcagaac cagaggcatc acaacctttt cccagaagga gtctaacacc
     5941 tgaaatgtca tgatcgtgaa catatctgcc tgggtctcta tagcttttga ataacatgaa
     6001 gaaagagtat tcggatgtac aaccgtatcc gcatctacga agcatagcca ctcgccgcgc
     6061 gcatgggctg cagcctggaa taacgcatga ggtttcccgg cccagccttt cggcaggtct
     6121 gagccgctga tcgggtgcag acgggaatcg agagctgcga tctctgccag gacaaaggga
     6181 gtcgcgtcgc ttgagcgatc atccaacaca atgacctcaa ggtttgggta atcctgtgca
     6241 agcagtgcct cgacacaggc gcggatattg cgctcttcat tgcgcgccgg aatgcaaaca
     6301 gaaattaaag gtgctggtga tggagggggt gcaggttcga cgacgatatc aagatgatac
     6361 tgattgtgca gccagataat gacaacaata ccgctaataa aaaacgcgga agatagcagg
     6421 aaatagatca tgaaccgggg taggaagtta ggtgactcgt acggaggagc cgcgatcggt
     6481 tttttcaact tcaatacggc tggggaaggc gtctttcagt tcatcgaggt gagtaatcac
     6541 cagaattttt gcgaaatcat tcttcacaag attgatcgcc tcgagtagac gttgacgccc
     6601 gcgtgtatcc tgtgaaccga aaccctcatc gatcacaagc gtttgcaaac gcgcaccttt
     6661 tcgctgtgcc aggatctcag acagcgcaag gcgcaccgcg aagttaacgc ggaaggcttc
     6721 gcctccggaa tacatctcat aggcacggat gcccgccgcg tcactgatct gaatatcgag
     6781 tgtttctttc agatcatctc gtttcttgtc cttgtactcg gcctgggtca caaatcgaat
     6841 ggacatctgc ccatcgctca aacggtcgag caggtcattt gccttggttt caatttgtgg
     6901 taacgcctgc tcgatcagca aagccgggac cccatccttg ccaaaggcgc gttcgagcgt
     6961 cttatgctgg gcgatgtgtt tgaccagctc ttcacgatcc gtttcgaatt gtaattttcg
     7021 tgaccgctgg acggtcagca cgtccactcg ctgttgcgcg ccgcccagct cgctgcgctt
     7081 tttattttcc tcttcgcgca accgataaag ctctcgctca gcagaatcca gatttggtgc
     7141 ttctgcctct gcagattcaa gattcttttt tgcggt
//
LOCUS       DASTJP010000073         7401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_248997, whole genome shotgun sequence.
ACCESSION   DASTJP010000073 DASTJP010000000
VERSION     DASTJP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248997"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFQ23_02280"
     CDS             complement(<1..760)
                     /locus_tag="VFQ23_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET9905430.1"
                     /translation="MKQELFKERGQALILIVFAAIGLFAIAGLAIDGSIKFSDRRHAQ
                     NAADAAALAAALSLGKEESDWKLKALEVADGNGYDRNLVTNNVEVYRCDDTGASCGAY
                     DNSPDHVQVIITSHVNTYFARVIGVNATKNTVQAVALTRKGGPLFDGASIVSLNPSPN
                     CSGGSGSGGGSVDVGGNGSIYLNGGGIFVNSSASCGYSQTSCSVTIAAAGGAGITSAG
                     SAINDGCSSGMPQDTTAEQVVIPHEVYMPDRPGEC"
     gene            complement(791..1942)
                     /locus_tag="VFQ23_02285"
     CDS             complement(791..1942)
                     /locus_tag="VFQ23_02285"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HET9905431.1"
                     /translation="MKYSLQIQKMPQERGQAIVEFALVLMILMMVLVGILEVGRLMFF
                     YAAVNNASREAARYASAIGLDTNPGNGVTAEKYRFCEMIKNMAQRSAFFTTPTITISY
                     DHGPGTTAFDTCDGSVDTGVFINSGSNLDRVYVTVSANYSPLLNLLPISSRTITSSSA
                     RTILGYQDLQNVSSSSSNTATSSAPTTPGTTVPSNTPTTTITVTPTATGGGELVTMTP
                     LPSSTPTLVPTFTPTATITLTPTETMTPTLTFTPTITPTAMPGCDSITTGPLIIVSNV
                     MSLSFTNPHDPITVLNVQVVWNSVNGGAGSKPLALKSASLGGVFWAGTNSTGNLTITP
                     ATTVTIPGNNQTSAIMFTFDKNYQNRNNAESIVINLSTPGCEAYTIHRP"
     gene            2419..2568
                     /locus_tag="VFQ23_02290"
     CDS             2419..2568
                     /locus_tag="VFQ23_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET9905432.1"
                     /translation="MLFAPKEKGQGLVEYAIILALIAIVVIAVMRLLGPKIGNTFSTI
                     NSSLP"
     gene            complement(2636..3823)
                     /locus_tag="VFQ23_02295"
     CDS             complement(2636..3823)
                     /locus_tag="VFQ23_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905433.1"
                     /translation="MPKKNRESEQFDEMENESSAAESETTPSSPAETLSESTSSDTIP
                     QETAEFSPGEEISADAENESSSDELLDDVRRSLLEEEETDKNKKEKQWWRRIGRKVKS
                     AEQEPPPTLVEMDLPATSLQTDMVESEEQPSTPERYEDEINDLRDMLEAESPQSAAEP
                     SPAALEAELPAKAEREIDLEQLKEQAFRPRTTADVESDVRSIALEGGEEMFVEVESRP
                     RDTLKERREAFENVLKPYRSYIYMTVGFLGVVTAVILSFLIFNAYQQSRPQPVQEVSN
                     LPYPVAVSLPGGWSFDLGKGALQAGKWEPRGAEWLEGTEVCRWVSLPWSRQLEAVLRT
                     LNPNDPIELVMSNNDKLVYGVYSVDELTTEEIQKVDSNSPCLLLILTAADSETRWVLN
                     AKP"
     gene            complement(3837..5726)
                     /locus_tag="VFQ23_02300"
     CDS             complement(3837..5726)
                     /locus_tag="VFQ23_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905434.1"
                     /translation="MRTSRRRSFHRILFALGIALPGLIGILAVQAKAVTDERGKVEAA
                     LQAQVTSTVTSTSTPSLTATLPFQTSTRLPATSPPSLGNLLPSGLYAFIEAPIGTVPQ
                     PYVILSAFSALPSQISVTIQGFINSQEFICTAFPCAIDLPTSSRLVFTAYASSGEVSE
                     TVIATVSVTRTTDGYLVVIDSVSQFRSFNNSCGIAWGVFDEQNVAWDDFVQFPYELHT
                     KKTLHNLATQLLINGIVDASDCPAGGLSLGLSWPTACGLERASGAMIEWQNRFDDHIW
                     LASSQHGIPPKILKTLIEVETQFWPGNTRVFLDEYGLGQVNQLGMDVLLRRDPTLYQR
                     VCPSILSDCSRPYLSLEPQQQAMIRGAVVRLMDVTCADCEYGFDLNKAKESIPLVAML
                     FRANCQQVDYILSIADVPDPDADAATATAAVATIAAGGDTDTTSYEDLWRFTILSYHS
                     GLNCFQEALLATRKAHRPFTWENVEARLKCRGGADYVNAFMSNLFAFDFYRLDYTDTD
                     VVLAAPTIIPTRTPVSTPTLFVSNAMVRVQVYMDRNGNGTPEADEWIDAMAVLLETST
                     NEQITQRTQNGIAIFDMSGYTPGIGINVSLPGLYRNERLELPQEGEVTVTFVFDLPAL
                     PTTIP"
     gene            complement(5732..6940)
                     /locus_tag="VFQ23_02305"
     CDS             complement(5732..6940)
                     /locus_tag="VFQ23_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9905435.1"
                     /translation="MRVLLIDDEQFYFKLLQKPLKDAEYKLEYAKSGTEGLAKIPSFE
                     PELLIVDLKLPELDGFEILKRLRRDPKFSYIPVIVITSQDELSEKLKAFELGADDYLV
                     KPFQPEELVARMGILARRGRVSQNAQKEATGASDATIVTVHSLRGGLGCSSIVVNLGL
                     AFHKLWGKQTLLMDSVLTAGQVALLLDAKPTATWENLVGLKPGELDEAAIGEIMCSHK
                     SGTRYIASPRYPIAADTFTAEELKLLMESIKSQNDFIVIDTSHDFSDMTIQMLSMSDT
                     ILLVMAPEMASLRTTVSAMEIYDRLGIPLDKVKIALNNNSSNPAIKQAQLEKVLKRPI
                     DFVLPFEAGEVNRAINFGQPFILSNPDLPICLVLEKMAYSLSDESHKALPPAVPSAAW
                     KRVTSQLPKK"
     gene            complement(6967..>7401)
                     /locus_tag="VFQ23_02310"
     CDS             complement(6967..>7401)
                     /locus_tag="VFQ23_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9905436.1"
                     /translation="KYGFQTRAKDDSAQHKLLASELPPEKVLDVQKGIVQIGGDENMY
                     RELLSDFIKGLPKRLRAIQNYFAEKDLVELSRTAHNLSGIAANLGASQLSDCAKKLDK
                     QSVEGYTESLDPLVQEIQLVIVRLLEFSNTFLAEKQVNAESA"
BASE COUNT         1830 a         1909 c         1803 g         1859 t
ORIGIN      
        1 tgcattcgcc tggtctgtcc ggcatataga cttcatgggg gatcaccact tgttctgctg
       61 tcgtatcctg aggcataccc gagctgcatc catcattgat cgcgcttccc gcggaggtga
      121 tgcctgcccc accggctgca gcgatggtaa cactgcagct tgtttgtgaa tatccacacg
      181 acgcgctcga gttgacaaaa ataccgcctc cattcaaata aatggagcca tttccgccga
      241 catcaacact cccgccaccg cttccactgc cgccgctaca gttgggactc ggattcagcg
      301 atacaatgga cgccccatca aataatggcc caccttttcg ggtcaacgca acggcctgca
      361 ccgtattttt ggtcgcatta acgccgataa ctcttgcaaa gtaggtgttt acatgggacg
      421 ttatgatcac ctgcacatgg tcaggactat tgtcatatgc gccgcagctt gcgcctgtgt
      481 catcacagcg ataaacctca acattattcg tgaccaggtt gcgatcgtaa ccatttccat
      541 cggcaacttc caaagctttc agtttccagt ctgactcttc ctttcccagt gaaagtgcag
      601 cagccagcgc ggccgcgtca gcagcatttt gtgcgtgacg gcggtctgag aattttatgg
      661 agccatcgat cgccagaccc gcgattgcaa acaaaccgat cgccgcgaat acaataagga
      721 ttaaggcttg accacgttcc ttgaataatt cctgtttcat ctcattgctc cttcctgata
      781 attggcggtg ttaaggccta tgaatcgtgt aggcctcgca gccaggtgtt gacagattga
      841 ttacgatcga ttcggcatta ttccggttct gataattttt atcaaatgtg aacatgatcg
      901 cggaggtttg attatttccg ggaattgtca cagtcgttgc gggtgtaatc gtgagatttc
      961 ccgtgctgtt agtgccagcc cagaatacgc cacccagact ggcgctcttc agtgctaatg
     1021 gcttactccc tgcacccccg ttcacggaat tccaaacaac ctggacgttc aagaccgtga
     1081 tcgggtcatg cggattcgtg aacgatagac tcatcacatt gctgacaata atgagtgggc
     1141 cggtagtaat actgtcacag cctggcatgg ccgtgggggt gatggtaggt gtgaacgtca
     1201 gggttggtgt catggtttcg gtgggcgtta aagtgatcgt cgcagtaggc gtgaacgttg
     1261 gcaccagcgt gggagtgctg ctcggcaaag gagtcatggt gaccaattcc ccaccaccgg
     1321 tggcagtagg cgttactgtg atcgtcgttg tgggcgtatt cgaagggaca gtggtccccg
     1381 gcgttgttgg agcacttgag gtcgcggtgt tggatgagct agaagacaca ttttgaagat
     1441 cttgatagcc aaggattgtc cgggcacttg acgaagtaat ggtgcgtgaa ctaatcggga
     1501 ggagatttaa cagagggcta taattcgcgc tcacggtaac atagacccga tccagattcg
     1561 agccagaatt tatgaataca cccgtatcta ccgacccgtc acaggtatca aaggctgtcg
     1621 tccctggtcc gtggtcataa gaaattgtga tcgtgggcgt ggtaaagaaa gcggagcgct
     1681 gcgccatatt tttgatcatt tcacagaatc tgtacttttc cgcggtaacc ccgttaccag
     1741 gatttgtatc cagtccaatt gcagaggcat accgagccgc ttcacggctg gcattattga
     1801 cggcagcata aaaaaacatc aagcggccca cttccagaat gcctaccaac accatcatca
     1861 gaatcatcaa aacgagtgcg aactcgacga tggcctgtcc acgctcttgt ggcatctttt
     1921 gtatttgcaa agagtacttc atagtattca cctatacgct aacgactgca acgttgtccg
     1981 ggtcaaacag aaagcaaaac cgactcagaa aatgggtcgg tttcgcgatt gctcaccagc
     2041 aactagaccg cccaatggag attttgcgac cagattcatc cctggtatcc caggaatatg
     2101 acttcgatct ttaagtccca tcgatgcata atttcaattc aaaggcagga cgtgacaaat
     2161 gggtgaaagt tacaggtcca ggtagtacaa ctatcaccat atatagccat tttttcgata
     2221 aaagcatgga aacctgtaca tattgccctt ttgaatacaa gcctttcata ttaattcaat
     2281 tgcgaaatcg ggaatgcggg aggaaccgac cgtaaatcag gttttgaggt gtaaccttct
     2341 gtaaaacaga gagtcatgtc tctagaactg agggaggtcc tcagaaaatt catcaatctt
     2401 tttgaaagga aggttattat gttgtttgca cccaaggaaa aaggccaggg gctcgttgag
     2461 tacgcgatta tcctcgcgct catcgccatc gttgtgatcg ctgttatgcg cttgctggga
     2521 cccaaaatcg gcaacacttt cagcaccatc aacagttccc tgccgtaaca cgctcgtgag
     2581 agcaaaaaag agccccgcga caacgaacgc ggggctcttt ttatatcatt cgatctcatg
     2641 gtttggcatt taagacccag cgtgtttccg aatccgctgc tgtaagaata agaagcaggc
     2701 agggagaatt agaatcgacc ttttgtatct cttcggtggt cagctcgtca acagaataga
     2761 caccatagac cagcttatca ttattgctca tcaccagctc aatgggatca tttgggttca
     2821 gggtccggag cacagcctct aattgccggc tccagggcag tgaaacccag cggcaaactt
     2881 cggtcccttc cagccactct gcgccccggg gttcccattt gccagcctgc agcgcgccct
     2941 tgccgagatc aaagctccat cccccgggca ggctcacagc aaccggataa ggcagattcg
     3001 aaacttcttg caccggctga ggcctggatt gctggtaagc attgaagatc aggaaggaga
     3061 gaattaccgc ggtgaccaca cccaaaaatc cgacagtcat gtagatgtaa cttcgatacg
     3121 gcttaagcac attctcaaag gcttcccggc gctctttcaa cgtgtcccgc ggtcgagact
     3181 caacttccac aaacatctct tccccaccct cgagagcaat ggaacgaacg tcactctcaa
     3241 catccgccgt ggtgcgtggg cgaaacgcct gttcctttaa ctgttcaagg tcaatttccc
     3301 gctcagcttt cgcgggaagt tccgcctcaa gagcggcagg tgatggttcg gcggctgatt
     3361 gcggagattc cgcttcaagc atgtcgcgaa gatcattgat ttcatcctca taccgctcag
     3421 gcgtggatgg ctgctcctcg gactcgacca tatccgtctg caatgaagta gcaggcagat
     3481 ccatttcaac aagggttgga gggggctcct gttccgcgct ctttaccttt ctgccaatcc
     3541 gtcgccacca ttgtttctct tttttattct tgtcagtttc ttcctcttcg agtaaggacc
     3601 ggcgtacatc atccagcagc tcatcagagg aagactcgtt ctctgcatcg gccgaaatct
     3661 cttccccagg cgagaattcc gcggtctctt gcggaatcgt gtcggaggaa gtcgattcgc
     3721 tcaaggtctc agcgggactg gatggggtgg tttcggattc tgctgcggaa gattcatttt
     3781 ccatctcatc gaattgttct gattctctat ttttctttgg catggtcgtt ccttcattaa
     3841 gggatcgtcg taggcagtgc tggtagatca aatacaaatg tcactgtgac ctcaccttcc
     3901 tgcggcagtt ccaacctctc attacgatac aaacctggca ggctgacgtt gatcccgatc
     3961 ccgggtgtgt aaccggacat atcgaaaatg gcaatcccat tctgggttcg ctgggtgatc
     4021 tgttcattgg tggaagtttc aagcagcacg gccatcgcat caatccattc atcagcctcg
     4081 ggagttccat ttccgttgcg atccatatag acctgaactc taaccatggc attcgagaca
     4141 aaaagtgtgg gagtagaaac tggtgtgcga gtgggtatga tggtcggtgc tgcaaggaca
     4201 acgtccgtgt ctgtgtaatc caggcggtaa aagtcaaatg cgaacagatt actcatgaat
     4261 gcatttacat aatcagctcc ccccctgcac ttaagccgag cctcgacatt ttcccaggtg
     4321 aagggccgat gagcctttcg cgtggcaagc agggcttcct gaaaacaatt caagccacta
     4381 tgataagaga gaatagtaaa tcgccagaga tcttcatagc tggtcgtatc agtatctcca
     4441 ccggcagcaa tagtggccac ggcagctgtc gcggtggcgg catccgcatc tggatcagga
     4501 acatcggcaa tgctcaagat gtaatccacc tgctggcaat ttgccctgaa taacatggca
     4561 accaggggaa tgctttcctt tgccttattt agatcaaacc cgtattcaca gtccgcgcag
     4621 gtcacatcca tgagtctaac cacagccccg cgaatcattg cctgttgctg aggctccagg
     4681 cttaaatagg gtcttgagca atcggagagg atacttggac aaactctttg atataaggtg
     4741 ggatcacggc ggagcaaaac atccataccc agctgattga cctgacccag gccatactcg
     4801 tccaggaaaa cgcgtgtatt tccgggccaa aattgggttt cgacctcaat cagtgttttc
     4861 aatatcttgg gagggatacc atgctgactg ctcgcaagcc agatatggtc gtcaaaccgg
     4921 ttttgccatt cgatcatggc accggaagcc cgttccaggc cgcaggccgt tggccaactt
     4981 aaacccagac tcaaaccacc agccggacaa tcactcgcat ccacaattcc attgatcagc
     5041 aactgcgtgg caaggttatg gagtgttttc ttggtatgca gttcataagg aaattgtaca
     5101 aaatcgtccc aggcgacatt ttgttcatca aaaacccccc acgcaatacc gcaggaattg
     5161 ttgaaggaac ggaattgact tacggagtca atcaccacca aatatccatc cgtggtgcgt
     5221 gtaacagaaa cagtggcgat cacagtctca ctaacttcgc ctgaacttgc ataagctgta
     5281 aaaacaagcc ttgaacttgt cggcaaatca atagcgcagg gaaaagcagt gcaaataaac
     5341 tcctggctat tgataaagcc ctggatcgtc acggaaatct gcgatggcag ggcggaaaag
     5401 gcactcaaga tcacataagg ctgcggtacg gtgccaatcg gcgcctcaat gaaggcgtat
     5461 agaccagacg ggagtaaatt tcccagggac ggagggctag tagcgggcag ccttgttgaa
     5521 gtctgaaatg gaagcgttgc cgtgagagat ggagtggacg tgctcgtgac tgtagatgtg
     5581 acttgagcct gcaaagcagc ttccaccttc cccctttcat cggtcacggc tttggcttga
     5641 accgccagga ttccaatgag gcctggaagc gctattccga gggcgaagag aatgcgatga
     5701 aaggaacggc gtctactggt ccgcattgct cctacttctt aggcagttga ctggttaccc
     5761 gtttccaggc tgcggagggc actgccggcg gcagagcttt atgggattca tcactcagtg
     5821 aatacgccat cttctccagg acaagacaga ttggtagatc aggattgctc aaaataaacg
     5881 gctggccaaa attgatcgca cggttcacct cgcctgcttc aaagggcagg acaaaatcga
     5941 tggggcgctt gagcaccttt tccagctgcg cctgtttgat ggcaggattg gaggaattat
     6001 tgttgagagc gatcttcact ttgtcgaggg ggatgcccaa cctgtcgtat atttccatgg
     6061 cactcaccgt tgtacgaagc gacgccattt ccggcgccat caccagcaga attgtgtcgg
     6121 acatactcag catttgaatg gtcatatcgg aaaaatcgtg agaagtatct atgacaataa
     6181 aatcgttttg actcttgata ctttccatca acagtttcaa ttcctccgct gtgaaggtgt
     6241 ccgcggcaat ggggtatcgc ggagatgcga tgtaacgggt gccacttttg tgcgaacaca
     6301 taatttcccc tatggcggct tcatcgagtt ctccgggttt caagcctacc agattttccc
     6361 aggtcgcggt cggttttgcg tccagcagca acgccacctg tccagcagtc aaaacactat
     6421 ccatcagcag cgtctgtttt ccccagagtt tgtggaatgc cagccccaaa ttcacaacaa
     6481 tgctggaaca gcctaatccc cctcgcaggc tatggaccgt cacaattgta gcatcactgg
     6541 cgccggttgc ttccttttgc gcgttctggc tgacccggcc gcgccgtgcc agaattccca
     6601 tgcgcgccac cagttcttct ggctgaaaag gcttcaccaa ataatcgtcc gcacccagct
     6661 caaaggcctt tagtttctca cttagttcat cctgggaagt aataacgatg accgggatgt
     6721 agctgaattt aggatcacga cgcaagcgct taaggatctc gaacccatcc agctcaggca
     6781 acttcaggtc aacaatcagc agttctggtt caaaagaggg aatttttgca aggccctcgg
     6841 ttcccgattt tgcatattca agcttatact cggcgtcttt tagaggtttt tgtaataact
     6901 taaaatagaa ttgctcatca tcaatcagca ataccctcat aacttcctcc acggagcagc
     6961 aatcttctag gccgactctg cgttaacctg tttttcggcc agaaaagtat ttgagaattc
     7021 tagcaatcta acaatcacaa gttggatttc ttgaaccaag gggtcaagtg attcagtata
     7081 accttcaacg ctctgcttat caagcttctt agcgcaatca gataactggg atgctcccaa
     7141 attagcggct ataccactga ggttatgcgc tgtcctggat agctccacaa gatctttctc
     7201 tgcgaaataa ttttgtatgg cccgcagtcg cttagggagc cccttaataa aatcacttaa
     7261 cagttcccga tacatatttt catcgccccc aatctggacg atacctttct gaacatcgag
     7321 caccttttct ggtggtagtt cactcgcaag caatttgtgt tgggctgaat catcctttgc
     7381 gcgagtctga aagccatact t
//
LOCUS       DASTJP010000074         3749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_37374, whole genome shotgun sequence.
ACCESSION   DASTJP010000074 DASTJP010000000
VERSION     DASTJP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37374"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1276
                     /locus_tag="VFQ23_02315"
     CDS             224..1276
                     /locus_tag="VFQ23_02315"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HET9905437.1"
                     /translation="MRKTLLLVALALFLVACAPPVPATQDVSNPTATTATPGSMTADN
                     PEAAHMMEPISAPSVPLATETEGGQPLTFREEDGVKVFELTTKAVQWPILDGTTITAF
                     TYNGTVPGPMIRVTEGDQVRIVVKNELPDATTVHWHGVEVPNAMDGVPAVTQDPIEPG
                     QSFTYEFVAKPAGTFMYHSHFEGDVQVSAGLYAPFIIDPKEPEENPPAVDQTLMISES
                     LLRDGETFAPMPMTGMEPNFFTINGKAFPATEEITIKKGERVRLRLIGIGQFIHPMHL
                     HGIPFKIVATDGHTVPEAAQLTKDTVTVAPGERYDIEFVATEPGQWMLHCHILHHTTN
                     DNVEPGGLMLMINVVE"
     gene            1309..1707
                     /locus_tag="VFQ23_02320"
     CDS             1309..1707
                     /locus_tag="VFQ23_02320"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HET9905438.1"
                     /translation="MRRLILVNVIVLLAVFLTACGSGSTATSAPQDSGKPVNIKVDTN
                     PNPAAMGDIELVFNITDSNGKPIEGATVDVTATHIDMTGMDMSGAATEQRTGKYAINA
                     NFSMSGNWKLKVYVRKEGLDYSEEIELPVQ"
     gene            1740..2513
                     /locus_tag="VFQ23_02325"
     CDS             1740..2513
                     /locus_tag="VFQ23_02325"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HET9905439.1"
                     /translation="MSAVTRTISSKPKQSGFDWISKHWLVTFLIIYGIWVWLPFLAPI
                     LMKLGLTTAGNIVYFIYSFFCHQLPERSFFFFGEKTMYSLAEIQAAWQDTANPMILRK
                     FIGNEAMGWKIAWSDRMISFYTSVWLFALAWWPLRRKWKPLSWWTFALLLLPMIIDGT
                     THAVSDLQGIGLGFRDTNQWLVALTNNALSPAFYAGDALGSFNSWGRLISGLLAGLGI
                     VWIALPYIYQSQAINQQLDQLNYGAVLEQIKTKNPRAAG"
     gene            2473..3150
                     /locus_tag="VFQ23_02330"
     CDS             2473..3150
                     /locus_tag="VFQ23_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9905440.1"
                     /translation="MSKSKPKIRALLADDHHIVREGIRQLLESAKDIEVIAEAGDGEE
                     AQSLIEQLKPDVAVLDIQMPKASGIEVTRWVRAHLPQVGVLILTAYDDDPYVMAVLQA
                     GANGYILKTAKTEDLIQAVRDVYDGKSALDSSITKKLMSNLFKKQENQVVEPLTDREL
                     DVLRLAAKGYTNKAIGMQLNISDRTVQGHLAHIFSKMQAASRTEAVMRAVSFGWISQG
                     PDQITEE"
     gene            3147..>3749
                     /locus_tag="VFQ23_02335"
     CDS             3147..>3749
                     /locus_tag="VFQ23_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905441.1"
                     /translation="MKIDPRIPEPVQPIIKDYLRLTEQRLVGLINGSYAIGSIALGEF
                     NECFSDIDFITVLSRKASPIDLGHLRKIHQSIESTYPRWKMSGSYVQAGDLGKRGNDL
                     RPYPQFHDGMLHPAVHNGINSVTWWELKNHGIPIAGTRSGTLPFTVNWDVLITEMQEN
                     LNTYWRSWTRHPRRIAVLYANWGIQWAVLGVLRQFFTFREN"
BASE COUNT          974 a          920 c          947 g          908 t
ORIGIN      
        1 aacggccaga catggatctc gattgcagag tccgcggttc agcgggggga gccgaagctg
       61 gtggccagta ttcgacgcgt gctagaaggt gtcgtggccg cagaccgcta gctactgacg
      121 tataggccaa atggcttata tgcttatccg tcagattgct ctgccagatg aaagcggatc
      181 atcttatatt tagacaatac ttatccaatt cactcgagga aacatgagga aaactttatt
      241 attagtggcg ctggccttat ttcttgtcgc ctgcgcgccc cccgtccccg cgacgcaaga
      301 tgtcagcaat cccacagcca caactgctac gcctggcagc atgacagcag acaacccaga
      361 agccgctcac atgatggaac cgatttcggc acccagcgta cctcttgcca cggaaacaga
      421 aggtggacaa cctttgacat tccgtgaaga ggacggggtt aaggtctttg aattgaccac
      481 aaaagcagta cagtggccca tcctggatgg tacaacgatt actgcattca cgtataacgg
      541 taccgtgcct ggaccgatga tacgcgtgac cgagggagac caggtcagga ttgtggtcaa
      601 gaacgagctg ccagatgcta caacagttca ctggcacggg gttgaagtac ccaatgcgat
      661 ggatggcgtg ccagcggtaa cacaagatcc aatagaaccc ggtcaatcgt tcacatacga
      721 atttgtcgcc aaacctgccg ggaccttcat gtatcactcg cattttgagg gtgatgtgca
      781 ggtgagcgcc ggattatatg cgcctttcat tattgatcca aaggagccag aggaaaatcc
      841 gccagctgta gatcaaacac tgatgatctc cgagtctcta ctacgagatg gggaaacctt
      901 tgcccccatg cctatgaccg ggatggagcc caatttcttc acaattaatg gcaaggcgtt
      961 tcctgctaca gaggaaatta ccataaagaa aggcgagcgt gtccgattgc gcctgatcgg
     1021 catcggtcag tttattcatc cgatgcactt gcatggcatc ccttttaaga tcgtcgcaac
     1081 cgatggacat acagtccctg aagcagccca actgaccaag gacacggtta ccgtagctcc
     1141 aggcgagcgc tatgatatcg agtttgtggc gaccgaacca ggtcaatgga tgctgcattg
     1201 ccacatattg catcacacga cgaacgacaa cgtcgagcct ggcgggctaa tgttgatgat
     1261 caacgtggtt gagtaattca ttcaactttt ataaggagaa gcgaatcgat gcgtaggttg
     1321 attttggtta atgtgatcgt gctgcttgct gttttcctaa cagcgtgcgg gagcggaagt
     1381 acagctacat ctgccccaca agactctggg aagccagtaa atatcaaggt tgacacaaat
     1441 cccaatcctg ctgccatggg cgacatcgaa ttggtattca acattacaga ctccaatgga
     1501 aaacccattg agggtgcaac cgtcgatgta acggccaccc atattgatat gaccggcatg
     1561 gatatgagtg gagctgctac tgaacaaagg accggtaaat acgcaatcaa tgccaacttc
     1621 agcatgagtg gaaattggaa acttaaagta tatgtgcgca aagaaggatt agattattcg
     1681 gaagagattg agctgccggt tcaatagaca agcctcgcgt cttcctttca aggaaaacta
     1741 tgtctgctgt gacaagaacg atatcatcaa agccaaaaca atccgggttt gattggattt
     1801 cgaaacactg gcttgtaaca ttccttatca tttatgggat ttgggtctgg ctccctttcc
     1861 tcgcgccgat cctgatgaaa ttgggtctga ccacagcagg aaacatcgtc tatttcatct
     1921 actcgttttt ctgccaccag cttcccgagc gatccttttt cttcttcggc gaaaagacaa
     1981 tgtattccct ggctgaaatc caagccgcct ggcaagatac agccaatccc atgatcctgc
     2041 ggaagttcat tggcaacgaa gccatgggat ggaagattgc ctggtccgat cgaatgattt
     2101 cgttctatac tagtgtgtgg ttattcgcgc tcgcctggtg gccgctacgg cggaaatgga
     2161 aacccctttc ttggtggacg ttcgcgctcc tactccttcc gatgatcatc gatggcacta
     2221 cacatgcagt tagcgacttg caagggattg gtctcggctt ccgtgataca aatcaatggc
     2281 ttgtagctct cacaaataat gcactgtcgc ctgcgtttta tgcgggcgac gcgttgggtt
     2341 cgttcaattc ctggggccgc ctcatttccg gtctactagc cgggcttggg attgtttgga
     2401 ttgcgttacc atacatatat caatcgcaag ctatcaacca acaattggat cagctgaatt
     2461 atggagccgt ccttgagcaa atcaaaacca aaaatccgcg cgctgctggc tgacgaccat
     2521 cacatcgtcc gtgaagggat ccgtcaattg ctggagagcg caaaagacat tgaagtgatc
     2581 gccgaagctg gcgatggcga agaagcgcag tcgctgatcg aacagctcaa accagatgtg
     2641 gcagttttgg acatccaaat gcctaaggcc agcggcatcg aagtcacccg ttgggtacgc
     2701 gctcacctgc ctcaggtggg tgtccttatc cttaccgcat atgatgatga tccttatgtg
     2761 atggccgttc tccaggccgg cgcaaatggc tatatactca agactgccaa gacagaggat
     2821 ttgattcaag ctgtgcggga tgtatatgac ggaaagtccg cgcttgattc gtcgatcacc
     2881 aaaaaactca tgtcaaatct ctttaagaag caagagaatc aagtggttga gccacttacc
     2941 gaccgcgagc tggatgtatt gcgtcttgca gccaagggct acaccaacaa agcgattggc
     3001 atgcaattga atatcagcga tcgcaccgtg caggggcacc tggctcatat cttcagtaag
     3061 atgcaggccg caagccgcac ggaagcggtc atgcgcgccg tttcctttgg ctggatttct
     3121 cagggtccgg atcagatcac ggaagaatga agatcgatcc gcgtattcct gaacctgttc
     3181 aaccgatcat caaagattac ctgcggctca ctgagcaacg gctggttggt ttgatcaatg
     3241 gatcttacgc cattgggtct atcgctttag gcgagtttaa tgagtgtttt agcgatatag
     3301 actttattac ggtcctgagt cgcaaggcat cgccaataga tcttggacac ctccgcaaaa
     3361 ttcaccaatc catagaaagc acgtatccac gatggaaaat gtctgggagt tatgtgcagg
     3421 caggtgattt gggaaaacgt ggcaacgatt taagacccta tccgcaattc catgatggaa
     3481 tgctgcaccc tgctgtgcat aacggaataa attcagtcac ctggtgggaa ctcaagaatc
     3541 atgggattcc tatcgcaggt actagatctg gaaccctgcc ttttacagtg aactgggatg
     3601 tcctgatcac cgagatgcaa gagaatttga atacatactg gcgaagttgg acacgccatc
     3661 cccgacgcat cgcagtcttg tatgctaatt ggggcattca gtgggctgta ctcggtgtat
     3721 tgcgccaatt ttttacattt cgagaaaac
//
LOCUS       DASTJP010000075         6344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_29196, whole genome shotgun sequence.
ACCESSION   DASTJP010000075 DASTJP010000000
VERSION     DASTJP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29196"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFQ23_02340"
     CDS             complement(<1..286)
                     /locus_tag="VFQ23_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET9905442.1"
                     /translation="MPTLAELLANEIVKTGVDVVYGLPGGENVEVLDALRKCGVKFVL
                     VRNESSAIFMADTQARLTGGIGVALTTLGPGATNAYAGMAHAYLDRSPVLL"
     gene            447..1655
                     /locus_tag="VFQ23_02345"
     CDS             447..1655
                     /locus_tag="VFQ23_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET9905443.1"
                     /translation="MRPLENIRILDITRALAGPYCTMMLGDLGADVIKVERPVSGDET
                     RGWGPPFVGKPYGPYPGESAYFIAANRNKRSVTVNIQSQDGKEIVRKLAGISDVLVEN
                     YRTGDLDRLGLGYDDLHKLYPKLIYCSVSGYGRTGPYADRPGYDAIIQGEGGMMSITG
                     PVDGPPSRVGIPITDITSGMFAATAILAALRARDLTGEGQLVDISLFDAHIALLTNVA
                     SNYLVGGSPPRRLGNAHPNLVPYDSFSARDGWFILGVANEKQWGQLCDMLDRADLKTD
                     DRFTSNGKRVTNRDELVEELNSTFAQKNVDDWLADLVKAGLPCGRINSIPEVFEHPQA
                     QAREMTLETEHVSAGTVRLPGFPYKFSQTPAEILRPPPLLGQHTEEILTSLLNYSTEE
                     VASLKESRAI"
     gene            1751..2266
                     /locus_tag="VFQ23_02350"
     CDS             1751..2266
                     /locus_tag="VFQ23_02350"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905444.1"
                     /translation="MILPKQRDPRFITVRRGGSLQDADHRLLAIWAADCAQHVLHFFE
                     EMQPDDNRPRRAIESARAWARGEIKMSQSRAAGGHAMAAARELSGAARYAAYAAGQAG
                     VVAHVAAHELGAAAYAIRAVRAAAPEGLAGEAGRLECEWQRAQLPSQIRDLVLDDQKL
                     RNEICWFVFDC"
     gene            2541..2963
                     /locus_tag="VFQ23_02355"
     CDS             2541..2963
                     /locus_tag="VFQ23_02355"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9905445.1"
                     /translation="MAKAELKTKVNEASVTNFIDSVEDEQKRKDCFEILKLMKQITKE
                     EPKMWGASIVGFGSYHYKGKSGREGDWMLTGFSPRKQNLTLYLMGGFDTHADLLQKLG
                     KFTTGAGCLYIKKLDDVDKKVLKELVQASVKRMKQLSA"
     gene            complement(3067..4164)
                     /locus_tag="VFQ23_02360"
     CDS             complement(3067..4164)
                     /locus_tag="VFQ23_02360"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9905446.1"
                     /translation="MFLTTLVMAAMLLVACGAEETSTTVPGMTPSLEVPPTTADTTAT
                     SDGALLTETAVPGTDLTTTPEIPVTGEEDPSRLTNQLDFTVWNQDGEQVGEVNDMVID
                     LDETRISYVIVGTGGFLEIGEKDVLLPWDLLEIGTGDTANAFILQIDQETFNNAPDVD
                     LNTVLPQAGEPVADWDVDIRGYWDTGIIPNTPAAEATVDPNMTPTTDPGQATATPEMP
                     QGEQQGLALEGVVLASDLLGASVNLSPGQGQATAQGQDQATATPDASQGSGQGVGNFS
                     GNIVDLIVDVDTGEILFVVVNTTLDDGDHWIPIPLRFFQWAADYSGLLLNVNPAQLQE
                     APFFQADGFPNTTVDGWNSEFNAFWDTITPP"
     gene            complement(4512..5381)
                     /locus_tag="VFQ23_02365"
     CDS             complement(4512..5381)
                     /locus_tag="VFQ23_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET9905447.1"
                     /translation="MKIHLIDGTYELFRNHFGAPPKKSVDGMEVGATLGLMRSLLMLL
                     QSPGVTHVGVAFDHVIESFRNKLYAGYKTGEGVDPILLSQFPLAEKAVAAMGVVVWPM
                     VEFEADDAIATATARFKKSKSVEQILIGSPDKDLSQLVEGQRIVCWDRRREIIIDDAG
                     VVEKFGVTAESIPDYLALVGDSADGYPGIQGWGAKSTSVVLAKFKHIESIPKDPKKLP
                     LGLGRATTLLENLTANYEQALLFRDLSTLRKDVPMEEKLDDLKWRGAHARLKKLCQEL
                     RDEKIPERVQRWR"
     gene            complement(5470..5760)
                     /locus_tag="VFQ23_02370"
     CDS             complement(5470..5760)
                     /locus_tag="VFQ23_02370"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HET9905448.1"
                     /translation="MSFQAYLDNIEEKTGKAPKEFVAEAKKKKLTESRDIIAWLKKDY
                     GLGLGHARALDHVIRKGPNFELRQTTGTHRDDSGTLNLEGKAQKSKKVKKKK"
     gene            complement(5769..6149)
                     /locus_tag="VFQ23_02375"
     CDS             complement(5769..6149)
                     /locus_tag="VFQ23_02375"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9905449.1"
                     /translation="MADLTPSQNIDKFINELTDWRGKWIAKFRDLILKTAPEVTEEWK
                     WGVPVWAHKGNVVASGVFKDHVKLNFFKGASLSDPKHLFNAGLDAKATRAIDIGEGDK
                     LDEAALKELIRQAVSFNASGGKKK"
     gene            complement(6162..>6344)
                     /locus_tag="VFQ23_02380"
     CDS             complement(6162..>6344)
                     /locus_tag="VFQ23_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905450.1"
                     /translation="DLWTMLIPTFGQQILINQFLRSEPISEMNILISAGLTIVLSCII
                     AYIAIKLYQSERIITG"
BASE COUNT         1629 a         1615 c         1530 g         1570 t
ORIGIN      
        1 tcagcagaac gggcgaacga tccagatagg catgagccat tcccgcatag gcgttcgttg
       61 cgccgggtcc caaggttgtt agagccacac caattccccc ggtcagccga gcctgcgtgt
      121 ctgccatgaa gatggcagaa ctttcattac gaactaaaac aaacttgacg ccgcactttc
      181 gaagcgcatc cagaacttcc acgttttcgc cgccgggtaa gccatagaca acatccacac
      241 cggttttgac aatttcgttc gcaagcagtt cagcaagcgt tggcattcgc aaaattataa
      301 cgcaccgcaa agagagttaa agttcaagta ccaggtataa aagagtgaga ttgattgtcc
      361 tttgcgaaag atgggtttat actaccctcg taagatcctc ttcaattgat aataatccca
      421 aaaacgaaaa gagttgaaaa gcgatcatgc gaccccttga aaacattcga atattagata
      481 ttacccgagc gctggctgga ccgtactgta cgatgatgct gggagatttg ggcgcggacg
      541 tgatcaaagt ggaacgtccg gtcagcggcg atgaaacccg tggctgggga cctccgtttg
      601 tcggtaaacc ctatggtccc taccccggcg agtccgccta cttcattgcg gcaaatagaa
      661 acaaacgaag tgtgactgtt aatatccaga gccaggatgg aaaagagatc gtccggaagc
      721 tggcgggcat ttccgatgtg ctcgtagaaa attatcgcac cggagatttg gatcgactcg
      781 gacttggtta cgatgattta cataagctat accccaaact catttattgt tccgtcagtg
      841 gatatgggcg gaccggtccc tatgctgatc gtccgggata tgatgcgatc atacagggtg
      901 aaggcgggat gatgagcatc actggtcctg tggacggacc tccctcccgg gtcggcatcc
      961 cgatcacgga tatcacctct ggaatgtttg ccgccactgc gatcctggct gcgctgcgag
     1021 cacgcgatct gaccggtgaa ggtcaactcg tggatatctc attgttcgat gctcatatcg
     1081 cgctattaac gaacgtggca tccaactacc tcgtcggcgg ctcgccacct cggcgtctgg
     1141 gtaacgcgca tccgaatctc gtcccctacg attcgttctc cgcgcgcgat ggctggttta
     1201 ttttaggagt cgctaatgaa aagcagtggg gacaattgtg tgacatgctg gaccgcgcgg
     1261 atctgaaaac agatgatcgt tttacgtcca atggcaaacg ggtcacaaac cgagatgagt
     1321 tggtggaaga gctcaactcg accttcgcac agaagaacgt agacgactgg cttgcggatt
     1381 tggtcaaagc cggactcccc tgcggacgga tcaattcaat cccggaggtc tttgaacacc
     1441 ctcaagcaca ggcacgcgag atgaccctgg aaaccgaaca cgtttcggcg ggaactgtcc
     1501 gcctgcccgg cttcccctat aagttctctc aaaccccggc agaaatcctc cgcccgccac
     1561 ccctcctcgg ccaacatacg gaagaaatac taacgagctt attgaattat tcaaccgagg
     1621 aggttgcctc gttgaaagag agcagggcaa tttagttgaa aaatgaaatt catataacag
     1681 ctgagcccgt gatcagaagc tcatactcca ccgcttacag tgacaagaag aaacgcatta
     1741 tagctaacga atgatattac caaaacagcg agatccgaga tttatcactg tgcggcgcgg
     1801 cggttctctt caagacgccg atcatcgcct gcttgcgatt tgggcagcag attgcgcgca
     1861 gcatgtgctg cactttttcg aggaaatgca acccgacgac aatcggccgc gtcgagcgat
     1921 tgaatcagct cgcgcgtggg cgcggggcga gataaagatg tctcagtccc gcgcggcggg
     1981 tggtcatgcc atggcagcgg ccagagaatt gagtggagcc gcgcggtacg cggcctatgc
     2041 tgcgggccag gcaggagtag tggcacatgt cgccgcacac gagctcggtg cggcggctta
     2101 tgcaatcagg gctgtgcgtg cggcggcgcc tgagggtctg gcaggtgaag ctggtcgcct
     2161 ggagtgcgaa tggcagcgcg cacaacttcc aagccaaatc cgggatctgg tactcgacga
     2221 ccagaagttg cgcaacgaaa tctgctggtt cgtatttgat tgttgaactg actctgcaga
     2281 tcactctttc tgctcaacag taatacctcc tgcgggacca agtgcatcac tatcagttaa
     2341 aatctggtta gcggtattcg cagaatccga tttcgattag cccctcgtta tcgggtagtt
     2401 ttcccggctc aagcatggtt ccagcaaaag aggccactat agcaacagtt cctggaagtg
     2461 gaaaaaaggt tctgcataat tgttgaacga aacacaacta aagccaaaat tttgtataag
     2521 tccgaatatt ggaggctgag atggcaaagg ctgaactcaa gacaaaggtg aatgaagcca
     2581 gcgtgacgaa cttcatcgac agtgttgaag acgagcaaaa acggaaggac tgcttcgaga
     2641 tcctcaagtt gatgaagcaa atcactaaag aggagccgaa gatgtggggt gcaagcatcg
     2701 tcggatttgg cagttatcac tacaagggaa aaagtggacg cgagggcgac tggatgttaa
     2761 ctggtttctc accgcgcaag cagaacctga cgctgtatct gatgggcggc ttcgataccc
     2821 atgctgactt gttacagaaa cttggcaagt tcacaaccgg tgcaggatgt ttatatatca
     2881 agaaacttga cgatgttgac aagaaggttc tgaaagagct agtgcaggcg tcggtcaagc
     2941 ggatgaaaca gcttagtgca tgaaccacaa gtcaaagccg aataattcgg caacaggatt
     3001 ttaaatgaaa tggcgcagag agtcgattct ctgcgccatt ttgtttcgta ttatcgggag
     3061 tagtacttac ggaggtgtga tcgtatccca gaaggcattg aattcactgt tccagccatc
     3121 caccgttgta ttggggaacc cgtcagcctg gaaaaacgga gcttcctgaa gttgagctgg
     3181 gttcacattc aataggagtc cggagtaatc tgcagcccat tggaagaatc gaagcgggat
     3241 gggtatccaa tgatcaccat cgtcaagggt cgtattgacc acgacgaaaa gtatctcacc
     3301 ggtatcaaca tcaacaatca aatcaacgat gttgccgcta aagttgccca cgccctggcc
     3361 actgccctgg cttgcatctg gagtagcagt agcctggtcc tgaccttgcg ctgttgcctg
     3421 cccttgtccc gggctaaggt taacggaagc accaagaaga tcagacgcca agacaactcc
     3481 ctctaaagcc agtccttgct gttcaccctg aggcatctcc ggggttgccg tagcctgacc
     3541 cggatccgtc gtcggcgtca tgtttgggtc aacagttgct tcggcagcag gagtgttcgg
     3601 aatgatgccg gtatcccaat aaccgcggat atccacatcc caatcagcaa caggttcgcc
     3661 tgcctgaggc aggactgtat ttagatccac atcgggagca ttattgaaag tctcctgatc
     3721 gatttgaaga atgaacgcat tcgcggtatc acccgtgccg atctctagca aatcccaggg
     3781 caataaaacg tctttttcgc caatctccag gaagccaccc gttccgacaa taacataaga
     3841 tatcctggtt tcgtcgagat cgataaccat atcatttact tcaccaacct gctcgccatc
     3901 ctgattccac acagtgaaat caagctggtt cgttagacgg gaaggatctt cttcgcccgt
     3961 tacgggaatt tctggagtag tagtcagatc tgtcccagga accgctgttt ctgtaagcaa
     4021 ggccccatca ctcgttgccg tcgtatcagc cgtcgtcggt ggaacttcca gactgggtgt
     4081 catgccagga acagtagtgc ttgtttcttc ggctccacaa gccaccagca acatcgcagc
     4141 cattacgagt gttgttaaaa acacacttat ttttctcatc ttttaccttt tcgttttcct
     4201 ttcaagatta gtctagccgt agaatacggc taaaccatct aacaaggata ttcgatatgg
     4261 tggaataaac aatgggggat atccaaatcc catagtaggg aatgccttac atggaaaatc
     4321 agttgtgtta aagaatgggt tgagcaggca gagttgcaaa tctaatgccc ccaggcttaa
     4381 gtgctgaaat ggaagtgaaa ggagtttgtt tttttagaat tcgctcataa tagtcaatat
     4441 tgtgacgggc ccaggggttt catcataccg gcctccgatg tcgcagactt tcaaacatcc
     4501 aaaagttcgt attaacgcca cctctggact cgctccggaa ttttttcatc ccttaattcc
     4561 tggcaaagct tcttcagccg cgcgtgcgct cccctccatt tcaggtcatc aagcttttct
     4621 tccatcggaa catcttttcg cagagtggat agatctcgaa acagcaaagc ctgttcatag
     4681 ttcgccgtca gattttccag aagagtcgtc gcgcgaccta aacccagcgg aagttttttc
     4741 ggatctttgg gaatggactc aatatgtttg aatttcgcaa gtaccacaga tgtagacttt
     4801 gccccccacc cctgaatgcc agggtaaccg tctgctgagt cccctactaa cgcaaggtaa
     4861 tccggaatag attctgcagt gacaccaaac ttttcaacca cgcccgcatc atcaataatg
     4921 atttcacggc gtcgatccca gcaaacgatt cgttggcctt ccacaagctg agacaagtcc
     4981 ttatccggcg aaccgatcag gatctgctcc acgcttttgc ttttcttgaa gcgcgcagtc
     5041 gccgtcgcaa tagcgtcatc cgcctcaaat tcgaccatcg gccaaaccac cacgcccatc
     5101 gcagcaacag ctttttcagc cagtggaaat tgtgacagga gaatcgggtc aacaccttcg
     5161 ccggtcttgt atcccgcata gagtttgttg cggaacgact caatcacatg atcaaaagca
     5221 actcccacgt gcgttacgcc cggactctgc aatagcatca aaaggcttcg catgagccca
     5281 agcgttgcgc cgacttccat cccgtctaca gattttttcg gcggtgcgcc aaagtgattg
     5341 cggaataatt cgtaggtacc atcaatcaaa tgaattttca tttacagtat gtccttttct
     5401 tatattgact atgggaaccg attatggctt tcagcaacga tggtatccac tgagatttcc
     5461 tacaacgaat tacttcttct ttttgacctt cttgcttttc tgcgccttgc cttccagatt
     5521 caaagttccg gaatcatcgc gatgggttcc ggtcgtctga cgcaactcaa aattgggccc
     5581 ctttcgtatg acatggtcta gtgctctggc atggccgagc cctaatccat aatctttttt
     5641 aagccaggca ataatgtccc tggactcggt cagcttcttc tttttggctt cagcaacgaa
     5701 ctcctttggt gccttccctg ttttttcttc aatattgtct aaatacgctt gaaatgacat
     5761 gccggctcct atttcttttt accgccagat gcattgaagc ttactgcctg acggatcaat
     5821 tctttcaacg cagcctcatc aagcttatcg ccttcaccga tatcaatcgc acgagtcgcc
     5881 ttggcatcca gacccgcatt aaacagatgt tttgggtcac tcaaagaagc gcctttgaaa
     5941 aaatttagtt ttacatggtc tttgaacacg ccactggcaa ccacattgcc tttatgcgcc
     6001 catacgggca caccccactt ccattcttcg gtgacttctg gagcagtttt caaaatcaga
     6061 tcacgaaatt tcgcgatcca ctttccacgc cagtcagtga gttcattgat aaatttgtcg
     6121 atgttttggg atggagttaa gtctgccata gtttctcctt attaacctgt gataattcgt
     6181 tcactctgat aaagtttgat cgcaatataa gcaatgatac aagacaaaac gattgtcagt
     6241 ccagcagaaa tcaagatatt catctcggaa attggttcgg aacgcagaaa ttgattgatc
     6301 aaaatttgct gcccaaaagt tgggatgagc attgtccata aatc
//
LOCUS       DASTJP010000076        15240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1375885, whole genome shotgun sequence.
ACCESSION   DASTJP010000076 DASTJP010000000
VERSION     DASTJP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15240
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375885"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFQ23_02385"
     CDS             <1..878
                     /locus_tag="VFQ23_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905451.1"
                     /translation="ATGLSTGNTQNFTVTPTGGPLKVTLVWSDYPSTESASVNLVNNL
                     DLTVTSGANTYRGNVFSGGWSTTGGTADNRNNVENVYIQSPSGTYTVTVNAFNVPNGP
                     QKFALVVDNGTIAAPPASQNTGLLSPTAQLAATGGDNNGYESGAANAFADGGSEASDT
                     NSGTNKSTSCTDAGKDKHLYYNYNFSFTASQVQGIEVRLDARADASAGAPKLCVEISW
                     NGGTSWTAVKSTPNLSSTQATYILGGAADTWGRTWSTGDFSSGNFRIRVIDVSNNANR
                     DFFLDYLAVRVTYLP"
     gene            1038..1955
                     /locus_tag="VFQ23_02390"
     CDS             1038..1955
                     /locus_tag="VFQ23_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905452.1"
                     /translation="MQYDLCLPWYWEYDIDFVQFVEHACNAQGLTFWQITPDNLLESI
                     TALYKGEKSFQTLLERSQYDDRFEPILLWAKKYNRKRINPTEVSKWSEDKATMHLELM
                     GAGINTPYTILLPPFIEYPVIPELDLTPLGQQFVIKPSNEGGGEGVILGAFSMEQVLR
                     ARMQFPQQKYLIQATIMPRTIHGRPAWFRVFFANGKTYPCWWHPLSHVSATVTQQDEH
                     RFELTPLHKITERIASICKLDWFSTEIALTSKEFVVVDYVNDGIDTRVQSKAVDGVPD
                     EVIRNIAEQLVMIAKGETYDSRAGSGNSI"
     gene            1921..2991
                     /locus_tag="VFQ23_02395"
     CDS             1921..2991
                     /locus_tag="VFQ23_02395"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET9905453.1"
                     /translation="MTRARDLGIPFEGQPGEHNAITDVFGVTVGYATIIEGESARTGV
                     TIIHPRGGENPDPVFAAWFPFNGNGEITGAAWVEEGGFLEGPIGITNTHSVGIVRDTI
                     IQWQVEHDVIFQKWSCPLVTETADGWLNDMNGFFVKPEHVRHALDSAAAGPVQEGNVG
                     GGTGMLCYEFKGGSGTSSRKLPEKFGGWTVGAFAQTNFGVRYQLTIAGVPVGAHLKEH
                     PVWTDGENPYKPENGSLIVVLATDAPLLPHQLKRIAKRASLGMARTGSLGGNGSGDIF
                     LAFSTANPNAAIGGQNGIAELRALDNDHINPLFAASVFATEEAIINSMVAAEDMTGHN
                     GFSVKALPHDKLREIMTQYNRG"
     gene            3020..4945
                     /locus_tag="VFQ23_02400"
     CDS             3020..4945
                     /locus_tag="VFQ23_02400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9905454.1"
                     /translation="MNKTIAPYGSWKSPITADLITAKTIGFGQLNTDNDDIYWSEVRP
                     MEAGRTVVMRRAPDGTISECMPSEYYARTRVHEYGGGAYTVHDGVIYFCNFKDQHLYR
                     QRLDSTPELLTPGEGYRYADLVVDEKRDRVLCIREDHTGEGEEVNTIVSIDLNGNDNG
                     TILVQGNNFYSSVRLSPDGSKLAWLTWNHPNMPWDGTELWLADVKEDGTLQNAERVIG
                     SATESIFQPEWSPEGVLHFVAEDTGWWNLYRWQDGKAEALHPMEAEFATPQWIFGMST
                     FAFVSGNEIICAYTQNGLWSLALINTSTKQFTPIESPYTDMGDIHSGNGFVIFIAGSP
                     TQPSTIVRMSTETMKMEIIKQAFEVTVDQEFLSQARPISFSTTDGQTAHAIYYPPQSK
                     DYAAPESEKPPLTVISHGGPTSATGTTLRYGIQYWTSRGFAVLDVNYGGSTGYGREYR
                     QRLNGNWGVVDLDDCCNGASHLVREGLADPNRLAIRGGSAGGYTTLACLAFRDVFKAG
                     ASHFGVSDLEALAKDTHKFESRYLDSLIGPYPEREDLYYERSPINFAHKINCPVIFFQ
                     GDEDKVVPPNQSQSMFEAVRAREIPTAYILFQGEQHGFRKAESIKRALEGELYFYSKI
                     FNFDLAEEIEPIDIENL"
     gene            5196..5576
                     /locus_tag="VFQ23_02405"
     CDS             5196..5576
                     /locus_tag="VFQ23_02405"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HET9905455.1"
                     /translation="MEENKNARETRIKSYSNIWVGLIFIFGGAVVLLNQMDILPFELN
                     WWALFILFPAFGALTGAYNRYRSTNDLFEMGVMVPALVGLFMILLSVSLLLGNVIDMN
                     LRTYWPIILIVLGLGLILGRGRRS"
     gene            5601..6545
                     /locus_tag="VFQ23_02410"
     CDS             5601..6545
                     /locus_tag="VFQ23_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9905456.1"
                     /translation="MESILNVKDLKKNYGDFVAVKGITFDIKEGEIFSLLGPNGAGKT
                     TTISILSTLYLPTSGDATIGGYSVTTDPMAVKQIIGVVPQDLALYEDLTARENLIFWG
                     QMYGLSGKALTSRVDEVLAQIGLTDKAKNRVKTYSGGMKRRVNIGVGLLHKPRLLFMD
                     EPTVGIDPQSRRAILDTVKDLNKQGMTVLYTTHYMEEAQELSDRVGIIDHGELIALGT
                     QEELTKQVGQTDTLILHVGENDDPEPLAKSLKDLKDIQHAEASDHSVAIVTPSAEDIL
                     ASVVTKANERGIKIRSMEIREPNLEAVFLHLTGRALRD"
     gene            6649..7908
                     /locus_tag="VFQ23_02415"
     CDS             6649..7908
                     /locus_tag="VFQ23_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905457.1"
                     /translation="MKKLFLIGVKDLKLVFRDRAALTFMLLAPFLLTIGMGFVTGRFS
                     GGSSGLSDIPVIVVNLDNEELGDALVDVFSSEELADLVEPTTSSNPEAARRLIDEDQA
                     AAAIIIPEGFTRSIIPAEGTAFDESYVEPEPIRIEVYTNPSRPTSSGVVKAIVDEYIS
                     RVEEGRTSGMTSILQLAANGFITSENAETEAQTLFQNMEESEGTSITIKTNTQGAEAV
                     EFDILAYMAPGMAFLFLMYTVSYGGRSILQERAQGTLPRLLISPTSNAQVLGGKVLGI
                     FFTGVAQVGILILASTIFFRVQWGDPLGVAALILAAVLGATGWGMLITALARSPAQVS
                     SVGTALMLIFGILGGSFINLEQMPPLFHMLSRITPNAWGLDGFTTLALGGDLSNLIEP
                     ITALLIMSIVLFSASVLLFSRQGMVQK"
     gene            7905..9101
                     /locus_tag="VFQ23_02420"
     CDS             7905..9101
                     /locus_tag="VFQ23_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905458.1"
                     /translation="MRKIFAIAWKDAIIRFASASELLFFIILPLVFTFLLAGGTPSGD
                     EDPRIGLLVVDEAQTTISKQIINRLENSTAVRPESVTRDEAQSLFDDRRASAIFIIPS
                     GIDIESLQSGTAEVEFLQQPNNIDATIAQRAVLTATRDVSSAVSAAQTAVNQRESKQP
                     FTSNAEKEAYFGSSLELAQSIQQDVPERVTVVQGTTEDQVDYDPRANTSAGQLITWVF
                     IPLFGISALFAYERQQGTLRRLLTTPSHKATFLLGTISGQVAMALIQMSLLVGFGIFA
                     MQLGWGREPLALFLILFCSALAAAAFGTLMGTFIKTESQASGLSIMFGMVFALMGGCW
                     YPLELFPQTIQNAVKVLPTTWAMQGMLDLVLRGGGLTDVLPEAAVLLGFAVIFFSLGV
                     WRFRYE"
     gene            complement(9067..9966)
                     /locus_tag="VFQ23_02425"
     CDS             complement(9067..9966)
                     /locus_tag="VFQ23_02425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9905459.1"
                     /translation="MTNYLEVARVGKNEWWRYLISFPAILMIWFVVGSIPVILLMTYV
                     STDDNPVTNFSGTGFVGVPVILEFLFTMSSFIPFFVATLLAVRFIHTRPLKTLITGAT
                     QIRWTRLFAGAGAWLLIAALLSTVESFLYPGRYVLTFQPVTLLVFAVFALILIPIQTS
                     AEELFFRGYLLQWMGLRLKNRWLLALLNGALFFLPHAANPEMATDALLVGLGYFAIGF
                     FFTFVTLQDNGMELALGMHAANNLFAALFANYAVTALPSPSLFTVQVLDPVYSLVSLL
                     IGQIVFYLIFFYPTRSGIAKPPG"
     gene            complement(10083..10970)
                     /locus_tag="VFQ23_02430"
     CDS             complement(10083..10970)
                     /locus_tag="VFQ23_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HET9905460.1"
                     /translation="MIKSVEEYLDLLRVELQGSDAATIQDALADAEEHLRAALANQRE
                     TQPDLSEEQALELVIEQYGSPDETASAYREVEKRTLPQLTREPAKNTPSIGNFFGVYT
                     DARAWGSLLYMLIAFVTGVFYFTWAITGLSLSVSFSIFIFGLPFALLFLLSVQGLALL
                     EGRIVEALLGVRMPRRPLFTAKGLKWLERLKALVLDKHTWLSILYMIIQFVFGTVYFV
                     VFVTILSFSVSFIAVPFVQEILGEGVVMNNDIRYYVPTWSYPLLIFGGVLLWTAFMNI
                     ARGVGQLHGRMAKSMLVTE"
     gene            complement(10973..11332)
                     /locus_tag="VFQ23_02435"
     CDS             complement(10973..11332)
                     /locus_tag="VFQ23_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9905461.1"
                     /translation="MVDSPTLNKKFQKELNAGIASLVLLSVLNKSKKPMYGYQIAKLL
                     EEYSGDASFMKQGALYPVLHSLEGSGLLESEVEPSVSGPPRRYYEITSEGRKTLGEWQ
                     AVWKQMKSFVERAIQGV"
     gene            complement(11456..13474)
                     /locus_tag="VFQ23_02440"
     CDS             complement(11456..13474)
                     /locus_tag="VFQ23_02440"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9905462.1"
                     /translation="MSPDKVGRYKIKSELGRGGMATVYRAYDPSFDREVAVKVLPREM
                     VHNLVFRARFKRELKLIASLEHPAIVPVYDVGEEEDGRQYFVMRYMGGASLSDWIKKG
                     PLSLQDTAIIIERLGSALDYAHSKGIVHRDIKPDNVLFDETNHPYLTDFGIAKLTEAA
                     TSATGGGTMGTPAYVSPEQAQGAKVDGRADVYGMGVMIYEMLTGNKPYEADSPMSVAV
                     RHITEPVPDILQERPDLPFDVDIVIRTAMAKDKEDRFPTATDLARALGRVAFGDDRTT
                     PVNSKLLNKPNNSSNSRRRTLVIAGFGLLLAIASAFALSRQLPALSIDDTTPTVASTS
                     IPPTLNSTSTSAPEPTVAATATVMETEEVPTAAPPPGNADKVAFVAANQIYLMNIDGS
                     DLIQIRTDNSPKTNLHWMSDGRLVYVSKNCANIISVETRNIQELVCFNTDEILEGFRV
                     SPDGKWMAISVQRTLNILPFDPELLQGVETRFSLALLRENCFYNQYAFRDILWSKDGT
                     RLAARVVDTERVSSDQIFLLNVDIPNCDTTGPVRIDRIPGSDIEFDSASSKRITSFDW
                     NGDNLFLLDDSIRNDGFGDLYLYNSDTGVSEKINPIDGVCCYRDARWSPDGMYIIFVF
                     QRFDSPEVGLYYVPFGAWENGEALAPIELPDEFLTQRDKPQPVLRAAY"
     gene            complement(13549..14427)
                     /gene="folD"
                     /locus_tag="VFQ23_02445"
     CDS             complement(13549..14427)
                     /gene="folD"
                     /locus_tag="VFQ23_02445"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET9905463.1"
                     /translation="MTAQLIDGKLIAQQVRDEVARNVAERIASGKSKPTLATVLVGDR
                     VDSATYVASKQKACAELGMGSVSHKVPGDVSQAELENLVRSLNEDTSVHGILIQLPLP
                     AHLDEERVLQLVSVEKDVDGFSPINIGRLAQKGREPLFVPCTPYGCIYLLEKAGVTIE
                     GSNAVVLGRSNIVGMPAALLLIGKNATVTICHSRTRDLPSVIRQADILIAAIGKTEFV
                     RGDWLKPGAAVIDVGINSKPDATKKSGYRLVGDVNFAEAQEVAGFITPVPGGVGPMTI
                     AMLMKNTLRAAEIQDT"
     gene            complement(14430..>15240)
                     /locus_tag="VFQ23_02450"
     CDS             complement(14430..>15240)
                     /locus_tag="VFQ23_02450"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET9905464.1"
                     /translation="RTGYFELFTIAGIFLFRPSFSVIFNGQILSLLLLLVSVSILLFH
                     DQRWLGGGLALSLLSLKPSIGLPILALTAVWLFAKKQWQGILGIAVGCITLLLVGMLI
                     DPNWPIEYIGVSGDSFSKYFGLHPTLWGVVDKIFVQDAKSLVAGAVISTIIALTEVYI
                     LNRKDINNSPIEAFASILPAGLLIAPYSWSYDQILLAVPLIYLMIRIVFSNGDRMAVL
                     FLFGMVALSISLVVVAYMIRHDVWSFLNSFLIWIFMLILMPRSKVNPAGGE"
BASE COUNT         4024 a         3833 c         3716 g         3667 t
ORIGIN      
        1 ccgcaacggg actgagcacc ggcaataccc agaatttcac tgtcacaccg accggtggtc
       61 cactaaaagt taccctcgtc tggtcagatt atccttcaac ggaatcggca tctgtcaacc
      121 tggtcaacaa ccttgatctg acggtgacca gcggcgccaa tacctatcgc ggcaacgttt
      181 tctcaggcgg ctggagcacc acaggcggta ctgctgacaa tcgtaacaat gtggaaaacg
      241 tgtatatcca atctcccagc ggcacctaca cggtcacagt caacgccttc aacgtgccca
      301 acggcccgca gaagtttgcc ctggtggtgg ataacggcac aattgctgca cctcctgcct
      361 ctcaaaatac aggcttatta tcacccactg cgcaactcgc agccaccggt ggcgataata
      421 acggctatga gtcgggtgct gctaatgcat ttgcggatgg tggatcggaa gcttccgaca
      481 cgaacagcgg tacaaacaag agcacatcct gcaccgacgc cggcaaggac aagcatcttt
      541 actataacta caacttctcc ttcacggcaa gccaggttca aggcattgaa gtacggctcg
      601 atgctcgcgc cgatgccagc gctggagcgc ccaaactctg cgttgaaatc tcctggaatg
      661 gcggcacctc ctggacggcg gtcaagagta cccccaacct cagctcaacc caggctactt
      721 acattctggg tggagcggct gatacctggg gacgtacatg gtctacgggt gatttctcca
      781 gtggcaattt ccgcatccgt gtaatcgatg tttcgaacaa tgccaatcga gatttcttcc
      841 tcgattacct cgcggtcaga gtgacctatc ttccataagt aacaggatgt cgttgcgagg
      901 gcagtgattt ttggtctgaa gcaaacgctg ttttttcaga gagtgcttcc cctcgcaata
      961 atgatttagt tcgcaaaaag acggatgcct gtgtggtatc cgtctttttc tttcaactct
     1021 cccttataat tgaaccaatg caatacgacc tttgcctccc ctggtactgg gaatatgata
     1081 ttgattttgt tcagtttgtg gagcatgctt gtaacgcaca ggggctgacc ttctggcaga
     1141 tcacacccga taacttgctt gaatccatta ctgcgctcta taagggcgaa aaatcatttc
     1201 agaccctgct ggagcgatca caatacgacg atcgctttga gcccatactg ctgtgggcaa
     1261 aaaagtacaa caggaaacgg atcaatccga cggaggtctc aaagtggtcc gaagacaaag
     1321 ccactatgca ccttgagtta atgggtgcag ggataaacac gccttacacg atcctcctgc
     1381 ctccattcat tgaataccct gtcatccctg aactggattt aacgccgctc ggtcaacaat
     1441 tcgtgatcaa gccatccaac gaaggcggcg gcgaaggtgt catcctcggt gcattttcca
     1501 tggaacaggt gctgcgcgca cgcatgcaat tcccccaaca aaagtatcta attcaggcca
     1561 ccatcatgcc gcgcaccatt catggacgtc cggcctggtt ccgggtgttt ttcgccaatg
     1621 gaaaaacata tccctgctgg tggcatccat taagtcacgt atcagcaacc gtcacacagc
     1681 aggatgaaca tcgatttgag cttacgcctc ttcacaaaat tacggagcga atcgcatcca
     1741 tctgcaaact ggattggttt tctactgaga tcgcgcttac atcgaaggaa tttgtcgttg
     1801 tagactacgt caacgatggc atcgatacgc gcgtccaatc caaagcggtg gacggcgtgc
     1861 cggatgaagt cattcgaaac atagcagaac aactcgtcat gattgcaaaa ggagagacgt
     1921 atgactcgcg cgcgggatct gggaattcca tttgaaggcc agccagggga acataatgcc
     1981 atcacagatg tatttggcgt gaccgttgga tatgccacca ttatcgaagg tgaatcggca
     2041 cgcacaggag tcactatcat tcacccgcgc ggtggggaaa atccggaccc tgtttttgct
     2101 gcatggttcc cgttcaacgg caacggtgag atcacgggcg ccgcatgggt tgaagaaggt
     2161 ggctttctcg aaggcccgat agggatcacg aacacgcatt cggtggggat cgtacgcgat
     2221 accattatcc aatggcaggt cgaacatgac gtgatttttc aaaagtggtc ctgccctctt
     2281 gtcacggaaa ctgccgatgg ttggctcaac gatatgaatg ggttcttcgt caaacctgaa
     2341 catgtgaggc atgccctgga ttctgcggca gctggtcctg tgcaggaggg aaatgtgggc
     2401 ggaggcaccg gcatgttatg ctatgagttc aagggcggga gtggaacctc ttccagaaag
     2461 ttacctgaaa aattcggcgg ctggacggtc ggcgcgttcg cacagacaaa tttcggcgtg
     2521 cgctatcaac ttaccatcgc aggcgttccg gtgggcgcac atttgaaaga acatccggtg
     2581 tggacagacg gtgagaatcc atacaagcct gagaacggtt ccttgatcgt cgttctcgct
     2641 accgatgcgc cgctgcttcc tcatcaactc aaaagaatcg caaaacgcgc ctcgcttgga
     2701 atggctcgca cgggttcgtt aggcgggaat gggtccggcg atattttcct tgcgttctca
     2761 accgcgaatc ccaacgcagc aattggcggg caaaatggaa tcgccgaatt gcgtgcgctc
     2821 gacaatgacc atatcaaccc attgtttgcg gcttcggttt tcgctaccga ggaagccatc
     2881 atcaactcga tggtcgcggc ggaagatatg acagggcaca acggattctc tgtcaaggca
     2941 ttgccgcatg ataaattacg cgagataatg actcaataca atcgcggttg agatagagtg
     3001 gtctaatcat ggaggagaga tgaacaaaac aatcgcacca tacggaagtt ggaaatcgcc
     3061 tatcacagcg gacctcatca cggctaaaac aattggcttt ggtcaactca acactgacaa
     3121 tgatgacatt tattggagcg aagtgcgtcc aatggaggct ggccggaccg tggtcatgcg
     3181 ccgcgcgccc gatggaacca tcagtgaatg tatgccatct gaatactacg cgcgtactcg
     3241 cgtgcacgaa tatggtggtg gcgcatatac agttcacgat ggtgtcattt acttttgcaa
     3301 cttcaaggac cagcatcttt atcgccaacg gttagatagc acgcctgaat tacttacacc
     3361 gggggaaggt tatcgatatg ccgatttggt ggtagatgaa aagcgggatc gcgtcctctg
     3421 cattcgcgaa gatcacacag gggagggcga agaagtcaat acgatagtca gtatagatct
     3481 caacggaaac gacaacggaa ccatacttgt acagggcaac aatttttatt catctgtgcg
     3541 cctcagccct gatggaagta aattggcctg gctcacctgg aatcatccga acatgccgtg
     3601 ggatgggacc gaactctggc ttgcagacgt gaaagaagat ggcacacttc aaaatgcgga
     3661 acgcgtgatt gggtctgcca cagaatctat tttccaaccc gaatggtcgc ctgaaggagt
     3721 cttgcacttc gttgccgaag atacaggctg gtggaatctc tatcgctggc aggatggcaa
     3781 agccgaagcg ttacatccga tggaagctga gtttgctacc ccacaatgga tctttggcat
     3841 gtccacgttc gcttttgtgt ccggaaacga aattatatgt gcctatactc aaaatggact
     3901 ctggtctctg gcgttaatca atacctccac aaaacagttc acgccgattg agtcacccta
     3961 taccgatatg ggcgatatcc actcagggaa tgggtttgtc attttcattg cgggctcacc
     4021 gactcaacca tccacaattg tccgcatgag caccgaaacg atgaaaatgg aaatcatcaa
     4081 gcaggcattt gaagtaactg tggatcagga atttctctcc caagccagac cgatctcttt
     4141 tagcacgacc gatggacaga cggctcacgc gatctattac cctcctcaaa gcaaagatta
     4201 cgctgctcct gaaagtgaaa aaccaccgct aaccgtaatc agccacggcg ggccgaccag
     4261 cgcaacgggc acaacactca gatatggcat ccaatattgg acaagccgtg gattcgcggt
     4321 gctggatgtg aattatggtg gttccacagg ctacgggcgc gaataccgcc agcgtctcaa
     4381 tggcaattgg ggtgtcgtcg atctggatga ttgttgcaat ggtgcctcac accttgtaag
     4441 agaaggcctg gcagatccga atcggcttgc gattcgcggt ggcagcgccg gcggatacac
     4501 gactctggcg tgcctggcat ttcgcgacgt gttcaaggct ggcgcgagtc attttggcgt
     4561 tagcgatctg gaagcccttg caaaagacac tcataaattt gaatcgagat atctggactc
     4621 gttgatcggt ccgtatcctg aacgcgaaga tttatattat gaacgttcac cgatcaattt
     4681 cgcccacaag atcaattgcc ccgttatctt cttccaggga gatgaagata aggtcgttcc
     4741 acccaatcaa tcccagtcca tgtttgaagc cgtacgcgcc agagaaattc ccacggcgta
     4801 tattttgttt caaggcgaac aacatggttt tcgtaaggcc gagtcgatca agcgcgccct
     4861 tgaaggcgag ttgtacttct actcaaagat cttcaatttc gatctggcag aagaaatcga
     4921 gcccatcgac atcgaaaatc tataaccgat atccatcggc aaccgagaga aagttattct
     4981 taacacccct ttagcgatac aaatcaatga ggatcgtttg tattgacagt ctttaaatag
     5041 gaaatgacaa gaaatcaact tataccattc tagatttagt tactctctgt attaatgtat
     5101 gagcccaaaa ttggtaaaag aggctatact ggttttgaaa cgtcaggggg caatctccgt
     5161 acatagtcat aacaccaacg atcaaggaga tatcaatgga agaaaataag aatgcccgcg
     5221 agacccgcat aaaatcgtat tcaaatattt gggtgggtct catcttcatt tttggcggag
     5281 cggtcgtcct gctaaatcaa atggacattc tgccttttga attgaactgg tgggcgcttt
     5341 tcattctctt cccggctttt ggcgccctta cgggcgctta caatcgctac cgttccacaa
     5401 atgatttatt tgaaatgggt gtgatggtac ccgccctggt cggtctgttc atgattctgc
     5461 tgtcagtgag cctgcttctt ggcaatgtga tcgatatgaa cttgaggacg tactggccca
     5521 tcatcctcat tgtcctcggc ctgggtttga tcttaggccg tggacgaaga tcgtaagcaa
     5581 agatttcgcc gcagcgactt atggaatcca ttctcaatgt aaaagacctc aaaaagaatt
     5641 atggcgattt tgtcgccgtg aaaggcatca ccttcgatat caaagaagga gaaatcttca
     5701 gtttgcttgg cccaaacggg gcaggcaaga ctacaaccat ttcgatcctc tcaactttat
     5761 acctacccac atcaggtgac gcaactattg gcggttattc cgttacaaca gacccgatgg
     5821 ctgtaaagca aatcattggt gttgtaccgc aggatcttgc gctttatgaa gatttgaccg
     5881 cccgtgaaaa tcttattttt tgggggcaaa tgtatggact aagtggcaag gcactgacca
     5941 gccgcgtcga tgaagtgctg gctcaaattg gattaaccga caaagcgaaa aacagagtca
     6001 aaacttattc aggcggcatg aagcgacgcg tcaatatcgg tgttgggctg ctccacaaac
     6061 cacgcctgct tttcatggat gagcccacgg ttggaatcga tccccaatca cggcgtgcga
     6121 tcctggatac agtcaaagac ctcaataagc aaggtatgac tgttctctat acgacgcatt
     6181 acatggaaga agcgcaggag ctttcagacc gcgtcggcat tatcgatcac ggcgaactga
     6241 tcgcactggg gacgcaggaa gaactcacaa aacaagttgg ccaaactgac acattgatct
     6301 tacatgtggg cgagaacgat gaccctgaac ctctggcgaa atcccttaaa gacttgaaag
     6361 atattcagca cgcagaagca tccgatcaca gcgtggcaat cgtcacgcct tctgcagagg
     6421 acatcctggc ttccgtggtt accaaagcca atgagcgcgg gatcaagatc cgctccatgg
     6481 aaatccgtga gccaaatctt gaggcagtat ttttgcacct gactggacgc gcgttgagag
     6541 actaaagagt cgtcattgcg aggagcgttc gttgcgacga agcaatctga tgttgggcgg
     6601 ttacttgaga ttgcttcgct acgctcgcaa tgactttcta aaacgattat gaaaaaactt
     6661 ttcctcatcg gcgtcaaaga cctcaaactt gtctttcgcg atcgtgcagc actgaccttc
     6721 atgctgctgg ctccatttct actgaccatc ggcatgggct ttgtcaccgg gcgttttagt
     6781 ggcggttcca gcggactttc agatattccc gtgatcgttg tcaatctgga taatgaagaa
     6841 ctgggggatg ccttagtgga cgtcttctct tcggaggaat tggcagattt agtggaaccc
     6901 accacctcgt ccaaccctga ggcggcccgc cgtttaattg acgaggatca agccgccgct
     6961 gcgatcatta tcccggaagg atttacgcgt tccatcatcc ctgctgaagg cacagctttt
     7021 gatgaaagct atgtcgagcc cgaacctata cggatcgaag tgtatacgaa tccctcacgc
     7081 cctacgagtt caggcgtagt caaagccatt gtagatgagt atattagtcg tgtagaagag
     7141 ggtcgtacca gtggaatgac ttccatcctt caactggctg ccaatggatt cattaccagc
     7201 gagaatgcag aaacggaggc gcaaactctg ttccagaata tggaggagag tgaaggcaca
     7261 tctatcacca taaaaacgaa tacacaaggt gctgaggcgg tcgaattcga tatccttgca
     7321 tatatggctc cgggcatggc cttccttttc ctgatgtata ccgtttctta tggagggcgt
     7381 tcaatattgc aggaacgtgc acaaggtact ttgccacgtt tgcttatttc gcccacctcc
     7441 aacgcgcagg tgctgggtgg gaaggtcctg ggaatatttt tcacaggtgt tgctcaggtt
     7501 gggattctaa ttttggcaag caccattttc tttcgcgtgc aatggggtga tccattggga
     7561 gttgcagcct tgattttggc ggcggttctg ggtgcgacgg gatggggcat gctgatcacc
     7621 gcactcgcac gttcgcctgc acaagtatcc agcgttggta cggccttgat gttgatcttc
     7681 ggaatccttg ggggcagctt tatcaacctc gaacaaatgc cgcctctctt ccatatgctc
     7741 agcagaatca cgccaaatgc ctggggactg gatggattca cgactcttgc gcttggcgga
     7801 gacttaagta atctaattga gccgatcact gctctgctga ttatgagtat cgtgctattc
     7861 agtgcttctg tcctgttgtt tagccggcag gggatggtgc aaaagtgaga aagatttttg
     7921 caatcgcctg gaaagatgcc attatccgat tcgcgagcgc ctcagaattg ctgttcttta
     7981 ttattcttcc gcttgtattt acgttccttt tggcgggtgg aaccccctca ggagatgagg
     8041 acccacggat tgggctattg gtcgtggatg aggcacaaac cacgatttca aaacaaatta
     8101 tcaataggct tgaaaactcc actgccgttc gtccggaatc ggtaacgcgc gatgaagctc
     8161 aaagcctatt tgatgatcga cgagcctcgg ccatttttat cattccatca ggcatagata
     8221 ttgaatcttt gcaaagcggt accgcagaag ttgaattcct tcagcagccc aacaacattg
     8281 acgcaacgat tgcccaacgt gcagtcttga ccgcgacccg cgacgtcagc agcgcagttt
     8341 ctgctgcgca aactgccgtc aatcaacgcg agtcaaagca acctttcaca tccaatgcgg
     8401 agaaagaagc gtattttggg agttcgcttg agttagctca atccattcaa caggatgtgc
     8461 ctgaacgtgt aacggtggtg caggggacca cggaagatca ggtcgattat gacccacgcg
     8521 caaatacatc ggctggtcaa ttgattacct gggtatttat cccactcttc ggcatttctg
     8581 cattgtttgc ttatgaaaga cagcagggca cgttacggcg cttactaacc accccatctc
     8641 ataaggctac atttttactt ggcaccattt cgggtcaggt agcgatggcg cttatccaaa
     8701 tgtccctgct ggtagggttt ggaatttttg ccatgcaact gggttggggt cgcgagcccc
     8761 tggcattatt tttaatcctg ttttgctctg cgctggccgc ggccgctttc ggaaccctga
     8821 tgggcacttt catcaaaacc gaaagccagg cgagtggctt gagcatcatg tttggcatgg
     8881 tttttgcact gatgggtgga tgctggtatc cgcttgagtt gttcccacaa acgatacaaa
     8941 atgcagtaaa agttctgccg acgacatggg cgatgcaggg catgctggat ctcgtcctgc
     9001 gcggcggcgg attgacagat gtgcttcccg aagcagcggt cttacttggg tttgccgtga
     9061 tctttttcag cctgggggtt tggcgattcc gctacgagta gggtaaaaaa agatcagata
     9121 aaacacgatc tgtccgatca gcagcgaaac cagactatag accggatcga gaacttgaac
     9181 cgtgaacaac gaggggctcg gcagggcggt gaccgcgtaa ttggcaaata acgccgcaaa
     9241 caggttattc gcggcatgca tacccagcgc gagctccata ccattgtcct gcaaggtaac
     9301 gaatgtaaag aaaaagccga tcgcgaagta acccagccca accaggaggg catcagtggc
     9361 catctcaggg tttgcagcat gaggcaggaa aaacaacgca ccattgagca aggccagaag
     9421 ccatcgattc tttaggcgca gtcccatcca ctgcaggagg tagcctcgaa agaacagttc
     9481 ttctgcagaa gtctgaattg gaatgagaat cagggcgaag actgcaaaga ccagcaaagt
     9541 gaccggttga aaggtaagaa cgtaacgacc aggatataag aatgactcga ccgtggagag
     9601 cagtgcggca atgagcagcc aggcacctgc acccgcaaag agtctcgtcc agcgaatctg
     9661 agttgcaccg gtaatgagag tcttcaaagg acgggtatgg atgaaccgca cggccaaaag
     9721 ggtggccacg aaaaaaggta tgaatgagga catggtaaat aaaaattcaa gtattacagg
     9781 cacaccaaca aagcctgttc ccgaaaagtt agtcaccggg ttatcatccg tcgatacata
     9841 ggtcattaac aggatgaccg gtatggaacc aactacaaac cagatcatca ggattgcagg
     9901 aaaagaaatg agatagcgcc accattcatt tttcccaact cgcgcaactt ctaaataatt
     9961 ggtcatgaaa actccgatat cacaagatgc tataaagtgg atattctaac ctacgtcact
    10021 tactgaaaac caaacagcgg aaggcatcga aatgccttcc gctgtttggt ttgatttcca
    10081 cattattcag tgactaacat cgattttgcc attcgcccat gtaattgccc tacgccgcgc
    10141 gcaatgttca taaatgcagt ccacaataaa acaccaccaa agatcagcag gggataggac
    10201 catgtcggaa cataatagcg tatatcgtta ttcatcacca caccctcccc cagaatctcc
    10261 tgtacgaacg ggacagcaat aaacgaaact gaaaagctca atatggttac aaacacgaca
    10321 aaataaacgg ttccaaatac aaattggatg atcatgtaaa gaatggacaa ccatgtatgt
    10381 ttgtcgagta ccagagcttt cagacgctca agccatttca accccttggc tgtaaacagt
    10441 ggccggcgtg gcatgcgaac tcccaataat gcttccacaa tgcggccttc gagcaatgct
    10501 aatccctgca ctgacagcag aaagagcaaa gcaaaaggaa gcccaaagat aaaaatggaa
    10561 aatgaaacgg aaagtgataa ccccgtgatt gcccaggtaa aatagaaaac tccggtcaca
    10621 aacgcgatca acatataaag cagcgaaccc caggcacgcg catctgtata tacgccaaag
    10681 aagttaccaa tggacggcgt attctttgca ggctcacgcg tgagttgcgg tagggtgcgc
    10741 ttctcgactt ctcgataggc agatgcagtc tcgtcgggtg atccgtattg ttctatgacc
    10801 agttccaacg cttgttcttc cgacagatct ggttgtgtct cacgttgatt tgcaagagca
    10861 gctctgagat gttcctctgc gtctgcaagc gcatcctgaa tggtggcagc atcactgcct
    10921 tgaagttcaa ctcttaataa atccagatat tcttcaaccg acttgatcat cgtcatactc
    10981 cttgaattgc ccgttccacg aaagacttca tttgtttcca aacagcctgc cactcgccta
    11041 aggttttccg gccttctgag gtgatttcat aatacctgcg cggtggacct gaaacggacg
    11101 gttccacttc actctccaac aatccactgc cttccagtga atgcaataca ggatataacg
    11161 cgccttgctt cataaaggat gcatcgccgc tgtactcttc cagtagtttc gcaatctgat
    11221 aaccatacat aggcttcttg cttttattta acacgctcag caaaaccagg gaggcgatcc
    11281 ccgcatttaa ctctttttgg aattttttat tgagggtggg tgaatcaacc actatttctc
    11341 cttatgtata aggaatctat tagtataatt aacttaataa tagtagtaaa ttatgattat
    11401 gtcaagatta aataaaaaac tcccgctttc cggggcggga gaggtgtgaa gtttattagt
    11461 acgcggctct caatacaggt tgtggtttgt cgcgctgtgt taaaaattca tcggggagtt
    11521 cgatcggtgc cagcgcttcg ccattctccc aggcaccaaa gggaacgtag taaagaccca
    11581 cctccggact atcaaatcgt tgaaagacaa agatgatata catcccgtcc gggctccagc
    11641 gcgcatctcg ataacagcat acaccgtcaa tcgggtttat tttctcagat actccggtat
    11701 cactattata aagatatagg tccccaaacc cgtcattgcg gatggaatcg tccaaaagaa
    11761 agaggttatc gccattccag tcaaagcttg taatcctctt ggagctggcg ctgtcaaatt
    11821 caatgtctga cccggggatt cggtcaatac gcacaggccc tgtggtatcg cagttgggaa
    11881 tatccacatt cagcaggaat atctgatcgg aactcacacg ctccgtatcc acaacgcgag
    11941 cggccagtcg cgttccatct ttcgaccaga ggatatctcg gaacgcatat tggttataga
    12001 aacaattctc tctcagtaaa gcgaggctaa accttgtttc gacgccctgc aacagctccg
    12061 ggtcaaatgg gaggatattt aaggtgcgtt ggacgctgat tgccatccat ttgccgtccg
    12121 gcgatacgcg aaaaccttcg aggatctcgt ccgtattaaa gcagacaagc tcctgtatat
    12181 ttcttgtttc tacactgatg atatttgcac aatttttcga gacgtatact aaccgtccat
    12241 cagacatcca atgcaggttt gtcttagggg aattatccgt ccggatttga atcagatcgg
    12301 aaccatcgat attcatcagg tagatctgat tcgcggcaac gaaggcaact ttatcggcgt
    12361 ttccgggggg tggtgctgca gtgggtactt cctcggtctc catcacggtt gccgtcgcgg
    12421 cgaccgtggg ttcaggtgcg gaagtagaag tggagttcaa ggtgggtgga atggaagtgc
    12481 ttgcaaccgt cggcgtcgta tcatcaatcg acagagcagg caactggcga cttaaggcga
    12541 acgcggaagc gatggcaagc aataaaccga agcctgcgat gaccagcgtt ctgcggcgcg
    12601 aattggatga attatttggt ttgttgagga gcttagagtt aacgggagtg gtccggtcgt
    12661 cgccgaacgc cacgcgcccc agtgcccgtg ccagatcggt cgccgtaggg aatcgatcct
    12721 ctttatcttt tgccatcgca gtcctgatga cgatgtccac atcaaaagga aggtcgggcc
    12781 gctcctgtaa aatatccgga acaggttccg taatgtgtcg gacggccacc gacatcggtg
    12841 aatccgcttc atatggctta ttaccggtca acatttcgta gatcatcaca cccatgccat
    12901 aaacatcggc gcgtccatcc accttggccc cctgcgcctg ctcagggcta acataggcag
    12961 gtgtacccat cgtgccgccg ccggttgcag acgtagcggc ctccgtcagt tttgcgatgc
    13021 caaagtctgt gagataagga tggttggtct catcgaaaag gacattatcc ggtttgatat
    13081 cacgatgaac aattcctttg gaatgtgcat agtcgagcgc agatccaagc ctttcgatga
    13141 taattgccgt atcctgcaaa gataatggac cctttttgat ccagtctgaa agggaggctc
    13201 cgcccatata tcgcatcaca aaatactgtc gcccgtcctc ttcctccccc acatcataga
    13261 ctggaacgat agccggatgc tccaacgaag caattaattt aagttctcgc ttgaaccgcg
    13321 cgcggaaaac caggttatgc accatttcac ggggcaatac cttaacagct acttctctat
    13381 caaaactggg atcatacgcg cgatagaccg tcgccatacc gccacgtccc agttcggact
    13441 tgattttata tcttccaacc ttatccgggg acatgcacca agtatatcaa aatccacccc
    13501 ctgaaaaaca aggcggaacg gaattacaat gatgtaagcc gttacacttt aagtgtcttg
    13561 aatctccgcg gccctcaacg tattcttcat taacatggcg atggtcatag gtcccacgcc
    13621 gccgggcacg ggtgtaatga agcccgctac ttcctgcgct tccgcgaaat ttacatcacc
    13681 caccaaacga taaccgctct tcttggtagc atccggcttg gaattaatgc ctacatcaat
    13741 cactgccgcg ccaggtttga gccaatctcc gcgcacgaat tccgttttgc caatggcggc
    13801 gatcaggata tccgcctgac gaatgacgga aggtagatct cgggtgcgag aatggcagat
    13861 cgtgactgtt gcattcttcc caatcagcaa tagggctgcg ggcataccca cgatgttcga
    13921 gcgtcccaat acgaccgcgt ttgacccttc aatcgttaca cctgctttct caaggagata
    13981 gatacatcca taaggtgtac atggaacgaa caaaggttcg cgtccttttt gcgccagccg
    14041 cccaatattg atcggcgaaa acccatccac gtctttttcg acgctgacca gttgcaagac
    14101 gcgctcttca tccaggtgag cgggcaacgg caattggata agaattccat gtaccgacgt
    14161 atcctcattg aggctcctga ccagattttc cagctctgcc tgtgaaacat cgcccggtac
    14221 tttgtgactt actgatccca tacccaattc ggcacaggct ttctgtttgg acgccacata
    14281 cgtcgcagaa tccacacggt caccgaccag cacagtcgcc agcgttggct ttgacttgcc
    14341 cgaagcgata cgctccgcga cattcctggc aacttcatca cggacttgtt gtgcaatcaa
    14401 tttcccatcg atgagttgag cagtcatttt tattctcctc ctgcaggatt gacttttgag
    14461 cgaggcatca gaatcaacat gaatatccag ataagaaatg aattgaggaa actccagaca
    14521 tcgtgacgaa tcatatatgc tacaactacc agggagatgg agagtgcaac catcccaaac
    14581 aggaacaaga ctgccatccg atctccattc gaaaacacaa ttctaatcat caggtagatc
    14641 agcggaactg caagcaatat ctgatcgtag gaccaggaat aaggcgcgat gagcaaccct
    14701 gccgggagga tggacgcaaa ggcctcgatc ggggaattat tgatgtcttt tctattcaaa
    14761 atatacactt cagtcagggc gattatcgta ctaataactg cacctgcaac caggcttttg
    14821 gcatcttgta cgaagatttt atctacgacg ccccacaggg tgggatgcaa accaaaatac
    14881 ttgctaaaag aatcgccgct aacaccaata tattcaatag gccagttggg atcaatcagc
    14941 ataccaacca aaagcaaagt aatacatcca acggcaattc caagaatgcc ctgccattgt
    15001 ttcttagcga atagccacac ggctgtcaaa gcgagaatcg gaagcccaat acttggcttt
    15061 agactgagca gcgatagggc caatccaccc ccgagccaac gctgatcatg gaacagaaga
    15121 atcgaaacgg acaccagcag caatagcaga gagagaattt gcccgttaaa aatcacacta
    15181 aaggatggcc tgaataaaaa tataccagca atagtaaaca actcaaaata accggttcgt
//
LOCUS       DASTJP010000077        11496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1093380, whole genome shotgun sequence.
ACCESSION   DASTJP010000077 DASTJP010000000
VERSION     DASTJP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093380"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFQ23_02455"
     CDS             complement(<1..238)
                     /locus_tag="VFQ23_02455"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HET9905465.1"
                     /translation="MNEKPTGITANKKTSELTITWDDNHTSVYPFGLLRAACPCASCR
                     GGHENMKSEPDAEVFNIQLEDSPSTRMANVVAVGS"
     gene            complement(284..754)
                     /locus_tag="VFQ23_02460"
     CDS             complement(284..754)
                     /locus_tag="VFQ23_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905466.1"
                     /translation="MTVRQLFNKWVVLGALLLTGLLLLITAISIGLTGSRQTTDVGFA
                     PADVTVIPAPTLTSGAPPTPTIDPFAPTPAPIGIAIGNYVQITGTNGEGLRIRSEPGL
                     NSNPEFLGFDSEVFIIRDGPRAADGYNWWYLVAPYDESRAGWAAADFLAYIPPP"
     gene            complement(751..1716)
                     /locus_tag="VFQ23_02465"
     CDS             complement(751..1716)
                     /locus_tag="VFQ23_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9905467.1"
                     /translation="MRSAIIVYNPMAGRFPVKPFIKSAVRELQSAGWKVESAETQSGE
                     HTIELAKQAAAEKTDAVFAVGGDGTIGNVVNGLIDSDTALGVLPAGTANVWSIELGLS
                     PFAWTRPWVLRQNASILANAPTHAVDVGICNQLSFMMWAGIGLDAMTIHSLEPRLRLE
                     KFFAMPEFTARTIWNAAQWSGIRLRLWVDEHEVEGHFLLAVATNIRHYMGGLSNLSPD
                     AYIDDGLLDLWLFSGNNLGDALRHAYDLWRGNHMTSGAAHRITFHSLRVEADSPYWIQ
                     TDGEPRGSAQHTEITIKTRALKLLTPPRALDLLKNASLSYMGNTI"
     gene            1875..2951
                     /locus_tag="VFQ23_02470"
     CDS             1875..2951
                     /locus_tag="VFQ23_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9905468.1"
                     /translation="MNEGMMLNNRYQLLERLGTGGMSDVFRARDLMLERSVAIKILHE
                     TYSDDEAFQERFRQEARAAANLSHPNIVTVHDFGFDHGQLFIVMEYIPGKDLKTLLRQ
                     RGRFSVDDSIALMVQACAGIGYAHRAGLVHCDVKPHNMIVTPDSRLKVTDFGIARALS
                     TIQPDERADVVWGSPQYFSPEQAVGEAPSPASDVYSLGVVLYEVLTGALPFTAPSSDE
                     LARLHLEASPIPPSEYVPDIPTALEEIVLKVLSKEPAARYRTADQLGRVLLRFGNQRD
                     TVPSPPVHIPPEPAHANSYQQPEPPPVTPTPEPVISLPEINLPEIYAPEIDWASVALG
                     LVALLAVGGLIPFWIYIFFLYNPR"
     gene            3000..3662
                     /gene="rpe"
                     /locus_tag="VFQ23_02475"
     CDS             3000..3662
                     /gene="rpe"
                     /locus_tag="VFQ23_02475"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET9905469.1"
                     /translation="MNRYIISPSILSADFTRLGEEIAACESAGADWIHVDVMDGHFVP
                     NITMGQFIVATCRRVTKLPLDVHLMIEKPERHLESFAKAGASRLSVHVETCPHLQDTL
                     RQIKSFGCEAGVALNPDTPVQNIESFMTEADLILVMSVHPGFSGQGFIPTTIPKVAEI
                     RKKLDNLNSTAWVEVDGGVNVENVRELRDAGATAFAVATSVFKHPGGAKAGIKILRAK
                     IS"
     gene            complement(3904..4089)
                     /locus_tag="VFQ23_02480"
     CDS             complement(3904..4089)
                     /locus_tag="VFQ23_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HET9905470.1"
                     /translation="MAKCANCGKTTTFGHNRSFSQRATNRKFKPNLQKTLVMEKGRAV
                     HKVLCTKCIKAMSKSVA"
     gene            4266..4622
                     /locus_tag="VFQ23_02485"
     CDS             4266..4622
                     /locus_tag="VFQ23_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET9905471.1"
                     /translation="MGEETTSLGGIHISPIAVATIAYHATLQSYGVVGLTSKNLADGI
                     VKTITRDPSRGVSVNYNGEVLDIEIHVIVEYGTRINSVAESVANTVRFQVEKALGLRV
                     NTINVHVAGLRVSNTD"
     gene            4638..6305
                     /locus_tag="VFQ23_02490"
     CDS             4638..6305
                     /locus_tag="VFQ23_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET9905472.1"
                     /translation="MAVDTSRVEKLRKKQINGQSMKRLVEAGMTWLRTNQQTVNALNV
                     FPVPDGDTGTNMVLTMQSAWNEVKDSGHRSISDMANAVSKGALMGARGNSGVILSQLW
                     RGFARGVHGHDVMDGPTFAKAFAEARDTAYRGVVRPVEGTILTVAREVAEATAEGLKS
                     TNDAIDILEIAVKAADAAVQKTPDLLPVLKQAGVVDSGGKGFFFILEGMLRHVYGESL
                     ETPLMSVQPISSMQLNSAMEDVEEGQDYEVVVDFMPGNEFDLQRFYGRLEEMGTSIQV
                     GEGEGMYRMHIHVPLEKRYEPIDYIMGIGTITKVAMENLLAQLDDIEKSKSARISFAP
                     VEPGQIAVVVVSPGAGLSRIFASLGVAAVVEGGQTMNPSTQDILNSFENLPTDKIIIL
                     PNNKNIVMAANQAKDVTVKQVAVVPSRTVPQGLAAMLSLQPNGELEAVAATMTKSMSA
                     VQTGEITIATRTVEIDGVNVKDGQVIALLDGKLVASAASVEEGCLKLLEKAKADEREL
                     ITLFYGQEMPHAEANRIADEIREKYGDQEVEVQEGGQPHYQFIISVE"
     gene            complement(6425..7405)
                     /gene="mvaD"
                     /locus_tag="VFQ23_02495"
     CDS             complement(6425..7405)
                     /gene="mvaD"
                     /locus_tag="VFQ23_02495"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET9905473.1"
                     /translation="MTTATAQAFANIAFIKYWGNRDNTLRLPMNGSISMNLDGLYTRT
                     TVSFQPSLPFDELIINGHEIAGAGRDRVSYILDIIRGMANIHDRAEVMSENNFPSGAG
                     IASSASAFAALALAGSTAAGLNLSEQELSRLARRGSGSASRSIPGGFVEWQAGTGDED
                     SFAFSIAEPNHWNLVDCIAIVSTSHKKTGSTEGHGIAGTSKLQAARVADAQRRLDLCR
                     KAILERDFNAFASIVELDSDMMHSVMMTSTPALHYWKSESLEVMNCVRQWRSEGMQVC
                     YTVDAGANVHVICHQDQVLSLNKRLREIRGVEDVLIAHVGGPAKIVENNE"
     gene            complement(7547..10156)
                     /locus_tag="VFQ23_02500"
     CDS             complement(7547..10156)
                     /locus_tag="VFQ23_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9905474.1"
                     /translation="MHPWAEVSFGEWLKRRRKGLGLTQGQLAAQIKCSTIALKKIEAE
                     ERKPSAQMAERIAEILQIPQGERTSFLQFARGDWRAAPGSTIEHSPWLVSSTPPRSNL
                     PASINALIGRETEIRTLHSYLSDPIHRLITLIGPPGIGKTRLSLEAASGAMHGFQDGV
                     FFVALDSLDITDPITPTILHTLDVVDVKEGESAVEQLQATIGEKHMLLLLDNCEHLIE
                     DVAILVSCLLSACSQLVILTTSREALRVPGEQLYALQVLRIPMEGLSVHMETAAQFPA
                     LTLFAERARAVQADFALNSANIETVTRICQQSDGLPLAIELLAAEIRYMSPQALLSKM
                     SGQFIQSAEGMRAVADRQKTLQNAIRWSYNLLLPEKQKLFAYVSVFSGGFTLEMAERM
                     FSELIPDHSVSNLVISLFDKSLLRQMFTQEEADHSRFGMLATIHQYARQRLVMTKEQE
                     SIRHLHLRYFLELSEQAESGMRGFNASEWHRNLTHERNNIRAALEWAEKTDVEAGMYI
                     SSRLHDYWESFDLREGQRWLTRFVEKTESQTYPRARAKALFVLGDMLYDSRQFSQARS
                     LIQESLELYRVCDDKHSEVSAILSLAYILESQGDRQQSIEFTEQALALSQGLNDPWRQ
                     AFALANLGHLRPPAHKNRLDFWEKVIDLFRKSGDSQALADRLGGLGLELVEDGDIDNA
                     QKYLAESTLILEQLHTKAQWMPAKYAYSLIAVQAGNYEQARALLGEIMLITNELGGQM
                     SYFSTKLRLGHLAIREGKLTEARNVFAESIQMFQRERIGDNIVFTLEGVASLFVAIGK
                     FEHASGLFGWADATRKRDSLARPHIEQVDVDRDIQMIVQNIGNTAFESAYDVGRTMSL
                     EDAITYALEDLDG"
     gene            10298..10849
                     /locus_tag="VFQ23_02505"
     CDS             10298..10849
                     /locus_tag="VFQ23_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905475.1"
                     /translation="MDRKSILIILIFLVASACVGQSSQTTQTDAKGVAVTSYQTVLGK
                     LLNDKDVANFIATNHCTSAAEFKVCKEAGMALWVDTSQIVKMVYMYSGNSDAFRRYRG
                     PLPFGLSFYDPMWRVKEKLRGLNVDDSLSLQDSWQAGLPDEEGSPDHIHYWAIYKRYG
                     MTVLYNSAGYDEDAYIYAIVVGE"
     gene            complement(11282..>11496)
                     /locus_tag="VFQ23_02510"
     CDS             complement(11282..>11496)
                     /locus_tag="VFQ23_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905476.1"
                     /translation="GVAVSSGVLVEAISLEVEVTGTVEVILTTGIEVEAGADVGMGVA
                     AIWGVQAVTKRLAITINIWNKRFVNI"
BASE COUNT         2854 a         2814 c         2946 g         2882 t
ORIGIN      
        1 aggatcctac tgcgacgaca tttgccattc gcgtcgaagg cgaatcttcg agttggatat
       61 tgaagacttc ggcgtctggt tctgatttca tgttttcatg accgccacgg caggaggcac
      121 acgggcacgc cgcgcgtaat aacccgaacg gataaacact cgtgtgatta tcgtcccatg
      181 taattgtcag ttcgcttgtc tttttatttg ctgtgatgcc agttggtttt tcgttcatag
      241 ttcctcggta gggacgggcg gccgcccgtc cctacattga aatttatggt ggtggaatat
      301 acgctagaaa gtctgcagct gcccagcctg cccgcgattc atcatatgga gccacaagat
      361 accaccagtt atatccatct gcggcgcgcg gaccatccct gatgatgaag acctccgagt
      421 cgaagccgag gaattccggg ttgctgttca gtccgggttc agaacggatg cgaagtccct
      481 ctccgttagt tccagtaatc tgcacatagt taccaatcgc aattcctatc ggagctggag
      541 ttggagcaaa cggatcaatg gtcggtgtgg ggggcgcgcc ggaagtcagc gtgggagctg
      601 ggatcacggt cacgtctgcc ggagcaaagc cgacgtcggt ggtctgacgt gatccggtga
      661 gtccgatcga aatggcagtg atgaggagca ggagtccggt taataagagg gctccaagaa
      721 caacccactt attgaagagt tggcgaacgg tcatattgta ttgcccatat acgataagga
      781 tgcattcttc aacaggtcca gggcgcgcgg cggcgtaagc aattttaaag cacgtgtttt
      841 gattgtgatt tctgtatgtt gcgcagagcc gcgcggttcg ccgtcggtct gaatccaata
      901 gggtgaatcc gcttccacgc gtagagaatg aaatgtgatg cgatgtgctg cacccgatgt
      961 catatgattg ccgcgccaca gatcataggc atggcgcaag gcgtcgccaa gattatttcc
     1021 ggaaaaaagc cagaggtcaa gcaagccatc atcgatatag gcatccggtg aaagattcga
     1081 cagaccaccc atatagtgac gaatatttgt cgcaactgca agcagaaagt gtccttccac
     1141 ttcgtgttca tccacccata agcgcaggcg gattccgctc cattgtgcag cattccagat
     1201 cgttctggca gtaaattcag gcatcgcgaa aaatttttcg aggcgaaggc gcggctcaag
     1261 ggaatgaatc gtcattgcgt ccagcccgat gccggcccac atcataaaag aaagttgatt
     1321 gcatatgcct acatccacgg catgagttgg ggcatttgcc agaatggatg cgttctggcg
     1381 caggacccac ggacgagtcc atgcaaacgg actgagtccc aattcgatgc tccaaacatt
     1441 cgctgtgcca gctggtagca cgcccaacgc agtatccgaa tcgatcaaac cattgaccac
     1501 gttgccaatc gtcccatctc caccgacagc aaaaactgca tctgtttttt ctgctgcagc
     1561 ttgttttgct agttcgatcg tatgctcgcc actctgagtc tcggcggact ccaccttcca
     1621 gcctgcagat tgtaattcgc gtacagccga tttgataaaa ggttttacgg gaaagcgccc
     1681 tgccatgggg ttatagacaa taatcgcact acgcatgaag acattatacc taacgccacc
     1741 cccacctatc ctcccccaaa tacgacgcaa agatgctctt gtccaaaata tatattgtcg
     1801 tatttggggg aggtgcctgc gaagcgggtg gagggggcgg tgggggtcga agtgcggtga
     1861 tataatcttt taccatgaac gaagggatga tgctcaacaa tcgctatcaa ctgcttgaac
     1921 gcctcggcac aggcggcatg tcagatgtgt tccgcgcgcg tgatctaatg cttgagcgtt
     1981 cggtagcgat caaaatcctg catgagactt attcagatga tgaagccttc caggaacgtt
     2041 ttcgtcagga agcacgtgcc gcggcaaacc tttcacaccc caacattgtc accgttcacg
     2101 actttggatt cgatcacggt cagttgttca ttgtcatgga gtacattccc ggcaaggacc
     2161 tcaagactct tcttcgccag cgaggacgat tctcggtgga tgattcaatt gcgctcatgg
     2221 tgcaggcctg cgcgggaatt ggttatgctc atcgtgccgg gcttgtccat tgtgacgtca
     2281 agccgcataa catgatcgtc acacccgatt cgcgcctcaa agttacagac tttggcatcg
     2341 cccgcgcgct ttctacgatc cagccggatg agcgcgccga tgtggtctgg ggatcgcctc
     2401 aatacttctc tcccgaacag gccgtgggtg aagccccttc accggcctcg gatgtttatt
     2461 cattgggtgt tgtgctgtat gaagtgctga cgggcgcgct gcctttcacg gctccatcca
     2521 gcgatgaact ggcgcgactg catctcgaag cgagtccaat tccgcccagt gagtacgtcc
     2581 ctgatattcc aactgcgttg gaggaaatcg tgttgaaggt tctctccaag gagcctgccg
     2641 cgcgctatcg cactgcagat cagctcggac gtgtcctcct tcgatttggg aatcaacgtg
     2701 acacagtgcc ttctccgcct gttcatatac cgcccgaacc tgcgcatgcg aattcctatc
     2761 agcaaccaga gccgcctccg gttacgccaa cgcctgagcc ggttatctca ctgcctgaga
     2821 tcaatcttcc cgagatctat gcccctgaaa ttgactgggc ttctgttgct ctcgggttag
     2881 tggccctgtt ggcagtcggt ggattaattc cgttctggat ttatatcttc ttcctttata
     2941 acccacgttg aaacatgtag gtgactgtca ccttgggagc gacagtcacc ttttatttta
     3001 tgaatcgata tattatctcc ccatccatcc tctcagcgga tttcacgcgc ctcggggaag
     3061 agatcgctgc ctgtgaatct gcaggagccg actggatcca cgtcgatgtg atggatgggc
     3121 attttgtccc gaacattacc atggggcagt ttattgtggc tacctgcagg cgggtgacaa
     3181 aactccctct ggatgtccat ttgatgatcg aaaagccaga acgccatctc gaatcgtttg
     3241 ccaaagcggg ggcgagtcga ttaagtgttc atgtggaaac ctgtccgcac ttgcaggaca
     3301 cgttgcgtca gatcaaatcc ttcggatgtg aagccggggt ggcgctgaac cctgatacac
     3361 ccgtgcaaaa tatagaatcg tttatgacgg aagcggatct gatccttgtg atgagtgtcc
     3421 acccgggttt ttcgggacag ggatttattc caacaacgat tccaaaagtg gcagagatcc
     3481 gtaagaaatt agataacctg aattcaacag cctgggttga agtggatggc ggagtcaacg
     3541 tggaaaatgt gagggaatta agagatgccg gagcaactgc ttttgcggtg gcgacctccg
     3601 tcttcaagca cccgggaggg gccaaggcgg ggataaagat attacgtgcc aaaatctctt
     3661 aatctctgaa aaattaaccg cgaagagcgc aacacttgca tgatttgcta tgcaaatcac
     3721 acgcacaccg ccccggttgg tcggtgccgg ggagtgcagg tgagtacggg aagatttttt
     3781 aaaggttttc cttggtgtcc ttagcgtact tcgcggttca agaaagaatc cctgaatacc
     3841 ctggcgaatt aaaacaaaaa tcacggcgct gttgccgtga cccttgttgc gtgtgattga
     3901 accttacgct acagacttac tcatcgcctt gatgcatttg gtgcagagca ccttatgcac
     3961 agcgcgaccc ttctccatca ccaatgtctt ttgcaggttt ggtttgaact tgcgattggt
     4021 ggcgcgttgt gagaaagaac gattgtgtcc gaaggtggtg gtcttgccgc agtttgcgca
     4081 ttttgccatt tgataaaatt cctttcttgc tataattccg attacgagta ccgttacgaa
     4141 agcacggcat tttaccatac atctctacct ttctcaaggc aagtgataaa atagagaatc
     4201 aaatgttcaa ggaggcatcc tgggcgccgg ggagggcacc ttgagcatca ttagaagagg
     4261 aatgaatggg tgaagaaaca acatctctgg gcgggatcca tatttcccca attgcagtag
     4321 cgacgattgc ataccacgct acgttgcaat cgtacggtgt ggttggactg acctccaaaa
     4381 accttgccga tggtattgtg aagaccatca cgcgtgatcc ttcacgcggg gtatcggtta
     4441 actataatgg ggaagttctc gatattgaga ttcatgtcat tgtcgaatat ggtacacgaa
     4501 ttaattcggt ggcggagagt gtagccaaca cagtacgctt tcaggtggag aaagcgttgg
     4561 ggttaagagt caatacgatc aatgtgcatg tagcaggtct gcgcgtgagc aatacggact
     4621 aaaggagaat ccaaatcatg gctgtcgata cctcacgcgt ggaaaagttg cgaaagaagc
     4681 agatcaatgg tcaatccatg aagcggctgg tggaagcagg catgacctgg cttcgcacca
     4741 atcaacagac agtcaatgca ttaaatgttt tcccggtgcc agatggggat acgggcacga
     4801 acatggtcct gacgatgcaa tccgcatgga atgaggtcaa agattcgggc catcgcagta
     4861 tcagcgatat ggcaaacgca gtatcaaaag gcgcgctgat gggcgcgcgg gggaattcgg
     4921 gtgtgatcct ctcccagttg tggcgcggtt ttgcgcgcgg cgttcacggt catgatgtaa
     4981 tggatggtcc gacgttcgca aaggcatttg cagaggcgcg tgatacagcc tatcgcggcg
     5041 tcgtccgccc ggtggaaggg acgatcctca ccgtcgcccg cgaagtggcg gaagcaacgg
     5101 cagaaggctt gaagtccacg aatgacgcga tcgatatcct tgaaatcgcg gtcaaggcgg
     5161 cggatgcagc agtccagaaa acgcctgatc tgttgcccgt cctcaagcag gcgggtgtgg
     5221 tggattctgg cggtaaggga tttttcttca tccttgaagg aatgctgcga cacgtgtatg
     5281 gtgaatcgct cgagacgccg ttgatgagcg ttcagcccat ttcgagcatg caattaaata
     5341 gtgccatgga agatgtggaa gagggacagg attacgaagt ggtcgtagac ttcatgcctg
     5401 gcaatgaatt tgatttgcag agattttacg ggcggctgga agagatgggc acgtcgattc
     5461 aggttggtga aggcgaaggc atgtaccgga tgcatatcca tgtgccactt gagaagcgtt
     5521 atgaaccgat tgattacatt atgggcattg gaaccatcac gaaagtggca atggaaaacc
     5581 tgcttgccca gctggatgat atcgagaaga gcaagtctgc gcgcatctcg tttgcgcccg
     5641 tagagcctgg gcagatcgcg gtggtggttg tttctccggg cgccggattg agccgcatct
     5701 ttgccagcct gggtgtggct gcggtggtgg aaggtgggca gaccatgaac ccatccacgc
     5761 aggatatttt gaattcgttt gagaatcttc cgaccgataa gatcattatc cttccaaata
     5821 ataagaacat cgtcatggct gcaaatcagg ccaaggatgt gacggttaag caagtggcgg
     5881 tggtgccaag tcgtacggtc ccgcaaggac tggccgccat gctatccctc caaccgaacg
     5941 gggagcttga ggcggttgct gcgacgatga ccaaatccat gagcgcggtg caaacaggtg
     6001 agatcactat cgcgacccgt acggttgaga ttgacggtgt caatgtaaaa gatggacagg
     6061 tgatcgcact gctggatggg aaactggtgg cgtcagccgc ttctgtggaa gaaggttgtt
     6121 tgaaattgct ggagaaggcc aaagcggatg agcgcgaact gatcacgttg ttctatggtc
     6181 aggagatgcc acacgcagaa gccaatcgca ttgcggatga gatccgtgag aagtatgggg
     6241 atcaggaagt cgaggtgcag gagggtggcc agcctcacta tcagtttatt atttcagtgg
     6301 aataacatat cgcgctgagc ggagccagac tgggcgaaga cgaagcgcct attgcaagcg
     6361 aatttaactt gtaggcatgc gcttcgtctc cattcgcctg aaggctcatt ccgctcagcg
     6421 cgaattattc gttattctca acgatttttg ccggtccgcc cacgtgggca atgagcacat
     6481 cttcgactcc ccgaatttca cgtaaccgtt tatttaaaga aagcacttga tcttgatgac
     6541 agatgacgtg tacattcgcg cccgcatcca ccgtgtaaca aacctgcatt ccttcacttc
     6601 gccactgtcg gacgcagttc atcacttcca gtgattcgga tttccagtag tgcagcgcgg
     6661 gtgttgacgt catcatgacg ctatgcatca tatcggagtc gagttccacg atggatgcaa
     6721 atgcattgaa atcccgctca aggattgcct ttcggcagag atccaaacgg cgctgcgcat
     6781 ccgccacccg cgcagcttgc aatttgctgg tcccagcgat gccgtgacct tcggtagaac
     6841 ctgtcttttt atgggaggtg ctgacgatgg caatacaatc caccaggttc cagtgatttg
     6901 gttctgctat cgaaaacgcg aaggagtctt catcacctgt ccctgcctgc cattcgacga
     6961 atccaccggg gatcgagcgt gacgctgaac cagacccacg gcgcgcgagg cgcgagagtt
     7021 cctgttcact taaattcaat cccgcggctg tgctccctgc caatgccaac gcggcaaatg
     7081 ctgatgcgga agaggcgatc cctgcgccgg agggaaaatt attttcactc atcacttcgg
     7141 cgcggtcatg aatattcgcc atgccacgga taatatccag aatatacgaa acacgatccc
     7201 gccccgcgcc agcaatttca tgtccgttga taatcagttc atcaaatgga agtgatggct
     7261 gaaagctgac cgttgtgcgt gtgtagagtc catccaaatt catcgagatg gagccgttca
     7321 taggaaggcg caaggtatta tcacggtttc cccaatactt gatgaaggcg atattcgcga
     7381 aggcttgagc ggtggcggtg gtcatgctgc gatgataaca ctttaaaatt agtccaatac
     7441 accaaattat tttgaagcaa gccaatcact tgacgtgatt ggaaacaggt gtcaaaaacg
     7501 caaacagctt attacaaaat aagtgccaga aaaatctaca agaaaatcag ccgtccaaat
     7561 cctcaagcgc ataagtgatt gcatcttcta atgacatggt gcggccaaca tcatatgcag
     7621 attcaaatgc ggtgttgcca atattctgaa caatcatctg gatatcacga tcgacatcaa
     7681 cctgctcgat gtgtggacgt gcaaggctat ctcttttgcg cgtggcatcc gcccaaccaa
     7741 acagccctga cgcatgctcg aacttgccta tggcaacaaa caggctggct actccctcga
     7801 gtgtgaatac gatattatct ccaattctct cccgctgaaa catttggatg ctttctgcaa
     7861 agacattgcg tgcctcagtg agtttgcctt cgcgtatggc aagatgtccg agacgaagct
     7921 tggtcgaaaa ataagacatt tgcccaccta attcattggt gatcagcatt atttctccga
     7981 gtagagcacg agcttgttcg taattcccag cctgaacagc aattagacta taagcatatt
     8041 tggcaggcat ccattgagcc ttggtatgca attgctctaa gattaaagtt gactcagcta
     8101 aatatttttg agcgttgtcg atatcgccat cctctacaag ttccaatccc aaaccaccga
     8161 gacgatcagc taaagcttgc gaatcgcctg atttgcgaaa caagtcaatt actttttccc
     8221 aaaaatccaa acggttcttg tgcgcgggag gtctcaaatg acccagattg gctagggcga
     8281 aagcctgtct ccatggatcg tttagcccct gagacagcgc caaggcttgt tcggtaaatt
     8341 cgatactttg ctgtctgtct ccctgggact ccaatatgta ggctaaacta aggatggcag
     8401 atacttcact atgtttatca tcacacactc ggtataattc gaggctttct tgtatgagag
     8461 agcgtgcctg ggaaaattga cgagagtcgt ataacatatc acctagcaca aacaatgcct
     8521 ttgctctggc cctagggtaa gtctgggact ctgtcttctc tacaaatctt gttagccatc
     8581 gctgaccttc tcgtaaatca aaactttccc agtagtcgtg taacctgctc gagatataca
     8641 ttcccgcttc cacatcggtt ttctcggccc attctaacgc agctcgaata ttattacgct
     8701 cgtgcgtcaa atttctatgc cattcacttg cgttgaatcc cctcattcca gattcagcct
     8761 gttctgaaag ttctaaaaaa tatcgcagat gtagatgacg gatactttct tgttcttttg
     8821 tcatcaccag tctttgacgg gcatactgat ggatcgttgc cagcatgcca aatcgtgaat
     8881 ggtccgcctc ttcttgagtg aacatttgcc gcaagagact tttgtcgaac aaggaaatca
     8941 cgaggtttga tacagagtga tctggtatta attcggaaaa catccgctca gccatctcca
     9001 aagtgaaacc cccggaaaag accgaaacat aggcaaacaa tttttgtttc tctggtaaga
     9061 gcaggttata gctccagcgg atcgcattct gtaaggtctt ttggcgatct gcaactgcgc
     9121 gcatgccttc agctgattgt atgaattgac cgctcatttt tgaaagcaat gcctgggggg
     9181 acatatagcg tatctctgcc gcaagaagtt caatggctaa tggcaaacca tctgattgct
     9241 ggcagattct tgtaaccgtt tcaatgttag cggaattcag cgcaaaatca gcttgtacag
     9301 cccgtgcccg ctcagcaaaa agggtaagtg cgggaaattg cgcggccgtt tccatatgca
     9361 cagataagcc ctccattgga atgcgcagta cctgaagagc ataaagctgt tcgccgggaa
     9421 cccgtaatgc ttccctgctg gtggtcaaga taacgagctg ggaacaggca gaaagcagac
     9481 atgagaccaa tattgcaaca tcttcaatga gatgctcaca attatcaagc aggagcagca
     9541 tatgcttttc cccaatcgta gcctggagct gttcaaccgc agactcccct tctttcacat
     9601 ctacaacatc aagagtgtga aggatcgtgg gggtaattgg gtcggtaata tcaagggaat
     9661 ccagagcaac aaaaaagacg ccatcctgaa atccatgcat ggctccgcta gccgcttcca
     9721 ggctcaaacg ggttttaccg atgccaggag gaccaatgag cgtgatcagg cgatgaatag
     9781 gatctgataa ataactgtgg agggtcctga tctcggtctc gcgcccaatc agtgcattga
     9841 tggaggcagg cagattcgaa cgaggcggcg tacttgatac cagccacggc gaatgttcaa
     9901 tcgttgaacc gggagcagcc cgccaatcac cgcgcgcaaa ttgaagaaat gatgttcgtt
     9961 cgccttgggg aatttgtaga atttcggcaa tacgctcagc catctgcgca gacggtttac
    10021 gttcctctgc ttcgatcttc ttgagagcga tggtcgagca tttgatctgc gccgcaagct
    10081 gtccttgcgt taatcccagt cctttgcgtc ggcgctttaa ccattctccg aacgatactt
    10141 ctgcccatgg atgcatttca attaattaga gtatcccttt tgtatccctc agggataccc
    10201 gtttcaaccc gtagccctgt aatgtatttc agataagcga agtataccat tcaggtaatt
    10261 tggaagttct tcttaatcca actctccgag gatatctatg gatagaaaat ctatattgat
    10321 cattctaata tttctcgtcg ccagtgcgtg tgttgggcaa tcttctcaga ctactcaaac
    10381 agatgcaaag ggtgtggcag ttacgagcta tcaaacggtc cttggtaaac tcttgaacga
    10441 taaggatgtt gctaacttca tcgcgaccaa tcattgcaca tccgcagcgg agtttaaggt
    10501 gtgcaaagag gcaggcatgg ctttatgggt ggataccagc cagattgtta aaatggtcta
    10561 catgtattcc ggaaactcag atgccttccg acgctatcga ggtccgttac cttttgggtt
    10621 gtcattttat gacccgatgt ggcgggtaaa ggagaaactg aggggcttaa atgtagatga
    10681 ctccttatct ttacaggact cgtggcaggc cggcctaccg gatgaagaag gctcaccgga
    10741 tcatatccat tattgggcaa tctataaacg atatgggatg acggtactct acaactcggc
    10801 cggatatgac gaggatgcat acatctatgc cattgttgtt ggtgagtagg tatcaattga
    10861 gatgctcacc ctgcacgccc ggcatggttt ccatggaagc taagaccgca ttcatttaat
    10921 aaggagcttc accggggtca aagaggtgac tgaaaccagt cctcgtctat ccggcaattc
    10981 tcagaaggtt gctcatgttc tttggagtct ggttaacatc accagcctga tctcgcaagc
    11041 ggccgcaatt cctcattcgt gcgcacaaaa ctgaatgcca aacaggggtt ttgtgctacc
    11101 gcgctgagca tcttaattct gcttgtattt gctatagtga aatggcttca ttgaattgac
    11161 tgatcaatgg tcacataact tattttcttc tcagtgcgca tgcattaata gctctatatc
    11221 aatttgtcct tgttccaact taaagaaaca agcccaataa aggatattaa tccaaaggag
    11281 atcaaatgtt cacgaatcgt ttgttccata tgttgattgt gattgcaagc ctcttggtca
    11341 cagcctgcac cccccagatt gcagccacgc ccattcctac gtctgctccc gcttcaacct
    11401 caattccggt ggttaagata acttcaacgg tgccggtaac ttctacttcg agtgagatcg
    11461 cctcgaccaa tacgccagag ctaactgcca caccga
//
LOCUS       DASTJP010000078         8245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1369286, whole genome shotgun sequence.
ACCESSION   DASTJP010000078 DASTJP010000000
VERSION     DASTJP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369286"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFQ23_02515"
     CDS             complement(<1..446)
                     /locus_tag="VFQ23_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905477.1"
                     /translation="MRGFRAMQKFFIKAIFFVISLSLIFNVLGTIADRTSPLDDWRAE
                     HHFRSAKLENGKNQIEAITLGNSHSDAIDYSVLGMEGQSLAFAAADLFEVEKYSGYLV
                     DFLPNLKRVFITISYYSFSRDNAVFEPFRTRRIGFYSTVPIWSPIAG"
     gene            complement(433..1893)
                     /locus_tag="VFQ23_02520"
     CDS             complement(433..1893)
                     /locus_tag="VFQ23_02520"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HET9905478.1"
                     /translation="MKFSSVQFLVFFPLVAMAFFSLPHRWRWILLLGASYVFYASWNP
                     KYLVVLLIITIIGYSSGRLLERDTRLKARRLILTLSLLANLGIFFFFKYFEISNYALH
                     TLFEGLGFAQLIPGFRILLPLGISYFTFQTMSYTIDVYRGVVETERHAGLYALFIAFF
                     PQVTAGPIARANQLLPQFHTVNDLDYEQVVSGLLRMAWGFFKKLVIADRLAIVVNTVY
                     GDPTAFTGFPLLIATYAFAFQIYCDFSGYADIAIGAAQVMGFKLQENFDQPYYAQSIP
                     EFWRRWHITLYNWLRDYIFYPLNRALRRSRPGSDSILVLALPPMLTMLASGLWHGTSW
                     TFIVWGALHGIYMVGSVLWNRAKNFITLPFSVPAWNATGLKVFTTFNLVCFAWIFFRA
                     NSIPEAWYILQHIFSNPEINVSQLNMMPVYDWLIALIALLVMELGQYGQQKYGSLRDI
                     IRCQPLWLRWSAYYALVVVIFMFGKLGEGEFIYARF"
     gene            complement(1898..2884)
                     /locus_tag="VFQ23_02525"
     CDS             complement(1898..2884)
                     /locus_tag="VFQ23_02525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9905479.1"
                     /translation="MKLPGVKTAKSFSRWVQARIYGGALILGYHRVAEVESDDYEVCV
                     SPEHFEQQMYALRKYTRPIRLAKLVQCLKVGAVPPSSVAVTFDDGYADNLYEAKPVLE
                     KYEIPATVFVCSGYQGREFWWDELDRLIMTSKADSSVLRLAVGEKPFDRDRHKVISET
                     SFADYRNLRRALYNFLLGLDVEERNQAMDAIRNWSGFSSSDVSSARSLSSVELLSLAD
                     GGLIEIGAHTRFHPILPRLVLEQQKDEILSSKLELEEILERKVEGFAYPHGRATLEST
                     RIVAEAGFSYACTSLQDVVRPGSDLHGLTRFWQKDVDGDKFMQTLNRWLKNS"
     gene            complement(2881..3630)
                     /locus_tag="VFQ23_02530"
     CDS             complement(2881..3630)
                     /locus_tag="VFQ23_02530"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9905480.1"
                     /translation="MLNQLRLLARRYLSRETRRRIARLAVWPPVGWVRFGDLRRLKPI
                     GSDYGNSRGLEIDRYYIEKFLTEFSGDIRGCVLEIKHNAYTRRYGGDRVTRSDVLHPV
                     EGNPDATIVADLTKADHLPSDLFDAIIFTQTLQVIYDIRTTIATLYRLLKPGGVLLAT
                     ASGMAQLSLEDFDKWGEYWRFTSKSARLLFEEVFSVGNVTVRSYGNVLAAISFLEGLS
                     VEDLKTKELDVVDRSYEILIAVRAVKPEYPK"
     gene            complement(3654..4616)
                     /locus_tag="VFQ23_02535"
     CDS             complement(3654..4616)
                     /locus_tag="VFQ23_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905481.1"
                     /translation="MSACLPDPKTPPLEWPISSVRLPAPSEWVDQGIILEAGAEGEWD
                     SYLWGGFAFSVIKKDDTYYLYYQGSSDYREEYDETVLWRAIGVATSYDGIHFTKYAGS
                     PVLTWFPNQSGEEGAVSSGVTLGKNGETFIYYGANTQESATTINADVRIASSPDGFKF
                     TDLGQVLNRNDRAVWGGGDELFSVAAIYDAEEWFVFYIPNGTSKSGLLGVAYGNHDES
                     HHESSAVTTGGKPIPVWGTAGHVKLTNDLYALVLNNVREKRTEVRLVSLQTPNIVSEP
                     VEVYKFDEVQQVVLLLDEENETWFMYYRTHENSYGVKLAPASMP"
     gene            complement(4660..5604)
                     /locus_tag="VFQ23_02540"
     CDS             complement(4660..5604)
                     /locus_tag="VFQ23_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9905482.1"
                     /translation="MKQISFLSRKFFRRTIQFFGSHFYFNPDPDIRRSILVAGTARSG
                     TTWLGDLIASQINCRILFEPFNPHLVPEYRNFNYFQYMRPGVKDQAFYIFAENLFMGK
                     IRNHWVDRHNERITSEFRLIKEIRANLSLKWLHNNFPEIPIIFLLRHPCAVVSSRMEL
                     GWATDLDIEPFLSQPNLIEDHLGPYLDLIKNVKTFEEKHAIIWSVSNVVPLRQFQPGE
                     LKLVYYEDLCTQTQVEMTSVFISLGKKAGVSLTRNFDRPSQTTRLTSPLVTGDDNLSG
                     WRKKMTSSQINNVLRIVDRFGLSHLYDDSLLPLKKDAP"
     gene            complement(5707..7098)
                     /locus_tag="VFQ23_02545"
     CDS             complement(5707..7098)
                     /locus_tag="VFQ23_02545"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HET9905483.1"
                     /translation="MSVAIYYLLDLRLQNIWLLFVSYIFIFSWDWTFAVVLALLTLIN
                     FLLALRLRIDDRGQRGLLWLGIGLNVSVWVFFRTAQFFLPELEEFIAALGLSTRAGGL
                     EILVPLGLSYYTLQTISYLVDVYRGQLDAESDWVNFSLYLAYFPKLLAGPIERARTFL
                     PKLTTPRLVNNRVLAQSMVLIFTGLTRKLIIADSLMASFRPGVFEYPAKYTPPELLIW
                     LVFYAFTLYNDFAGYTDIVRGISGFFGITLSANFRAPYFSRNFTEFWKRWHITLSEWL
                     RDYIYFPVSRELSHVRASYRKLANLILPPMITMLISGFWHGFNVNMLIWGGLHGTYLI
                     VERFSTLWRPAVPPQNQPIWRQGAGIAVVFLLTMLAWVPFHWDLPLALQIWQGLFDWS
                     DVAIRYRRMFLLLPLVFGSLIMDFMHYRYEDEFVFLNWPPMAKALCMAIVLFLVFIVT
                     GGDFEQPFVYQAF"
     gene            complement(7156..>8245)
                     /locus_tag="VFQ23_02550"
     CDS             complement(7156..>8245)
                     /locus_tag="VFQ23_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905484.1"
                     /translation="VALRSSTLQLRRVPGLTALYTVLFFILLVALAESAARSASFQAP
                     LTPPKMGSRHYQVGHKLALLDSLAQKVGDVDCIMLGSSMVDTGFSPAAFETEYKAITG
                     RDIHCFNFGIDASTAASTSALARIIVEDYHPRLLIVGTDARDYAVPIGDRDPAVVLNT
                     PWVAYRQDDFSFEGWLQENSYLYRYRQHLSRLARFQYENTIWSDTKLNYEILPNGFTP
                     LNTTGIYINDPPKPDDDSFEVTYYSRIYSPYQILHENLDAMERIMAYDESGTSVLLVE
                     MPVADGLYFFFGNGKSDYDRFTARVNELAALHNVPFWQTEPLDFIPNHGWADYSHLNR
                     VGAEVFSTWLGREIGWADLEGNNETFRP"
BASE COUNT         2334 a         2030 c         1933 g         1948 t
ORIGIN      
        1 cctgcaatgg gggaccaaat cggaacagtg gaataaaaac cgatccttct cgtccgaaac
       61 ggttcaaaga ctgcattatc acggctgaaa gagtaatagg aaattgtaat gaagactctt
      121 ttcaaattag gcaaaaaatc aaccaggtat ccggaatact tttccacctc gaaaaggtcg
      181 gccgcggcga aagccagcga ttgcccttcc atgcccaaca cagaataatc gatcgcgtcg
      241 ctgtgcgaat tccccaacgt gatggcctcg atttgattct ttccattttc cagctttgcc
      301 gagcgaaaat gatgttcagc gcgccagtca tccaatgggg aagttctgtc tgcgattgta
      361 cccagaacgt tgaatataag gctcagactg atcacaaaga agatagcttt gatgaaaaac
      421 ttctgcattg ccctaaaacc gcgcataaat gaattcccct tcacccagct tcccgaacat
      481 aaaaatgacc acaaccaacg cgtaatacgc ggaccaacgc agccagaggg gctggcaacg
      541 gattatatct cgcagactgc cgtacttttg ctgtccatac tggcccaatt ccattacgag
      601 aagtgctatc aaggcgatca accagtcata aaccggcatc atattgagct gagaaacgtt
      661 aatttccgga tttgagaata tgtgttggag gatataccac gcttcaggga ttgagttcgc
      721 gcggaaaaat atccacgcga aacagacgag gttaaaggtt gtgaagacct tgagcccggt
      781 tgcgttccag gcaggtacgg aaaatggcag agttataaaa ttctttgctc gattccataa
      841 tacactacct accatataga tgccgtgtaa ggcgccccaa acgatgaatg tccagctcgt
      901 gccgtgccag agtccgcttg ccagcatcgt caacatcggc ggcagcgcga gcaccaggat
      961 gctatccgaa cccggccggc ttcgccgcaa tgcgcggtta agcggataga aaatatagtc
     1021 acgcagccag ttataaagcg tgatgtgcca acgccgccaa aactcaggaa ttgattgcgc
     1081 ataatagggt tgatcaaagt tctcctgcag tttgaagccc atgacctgcg ctgcaccgat
     1141 agcaatatcg gcatagccgg aaaaatcaca ataaatctga aaggcaaacg cgtacgtagc
     1201 gatgagcagg ggaaaaccgg taaaagcagt cgggtcgcca tacaccgtat tgactacgat
     1261 cgcaagcctg tcggcgatca ccagcttttt gaagaagccc catgccatac gcagtagccc
     1321 gctgacgacc tgctcgtaat cgaggtcgtt caccgtgtgg aattgtggga gcagttggtt
     1381 tgctcgtgcg atcggtcctg cggtcacctg aggaaaaaag gcgataaaca aggcatataa
     1441 ccctgcgtgt cgctcggtct ccactacacc ccgatagaca tcgattgtgt aactcattgt
     1501 ctgaaaggtg aagtaggaga tacccagagg caacaggatt cgaaatccag gaatcagttg
     1561 tgcaaatccc aatccttcga ataacgtgtg gagggcgtag ttcgagatct caaaatattt
     1621 gaagaagaaa aatattccaa gattagcgag caggctcaaa gtcaggatta gccgccgcgc
     1681 cttcaggcgc gtgtccctct ccagcaaacg tccgcttgaa tacccgatga ttgttattat
     1741 caaaagcaca actaggtatt tgggattcca actggcgtaa aacacataac tggcaccaag
     1801 caacagaatc cagcgccaac gatgtgggag actgaaaaac gccatcgcca caagcggaaa
     1861 aaacacgaga aattgaacgg aactgaattt cacgatctca actattcttc agccatcgat
     1921 tcaaggtttg cataaatttg tcgccatcca catccttttg ccagaatcgc gtcaacccgt
     1981 gcaagtcact tcctggccgc accacatcct gcaaactggt acacgcataa ctgaagcctg
     2041 cttctgctac gatccgcgtg gactcaagcg ttgcccggcc atgtggatag gcaaaccctt
     2101 ccacttttct ttccaaaatt tcctccaatt caagtttgct ggaaaggatc tcatcctttt
     2161 gctgttcaag tacaagcctt ggcaggatcg gatgaaaacg cgtatgcgct ccaatttcga
     2221 ttaacccgcc atcggccaga cttaacagct cgacagaact tagcgaccgc gctgacgata
     2281 cgtcacttga tgaaaagccg gaccagtttc gaatcgcgtc cattgcctga ttccgctctt
     2341 cgacatccag tccaagcaga aaattataga gcgcacgacg gaggtttctg tagtccgcaa
     2401 aactcgtttc tgaaatcact ttgtgtcgat cgcggtcaaa gggtttttct ccgactgcca
     2461 gccgaagcac actcgaatct gctttcgaag tcattatcaa acgatctaat tcatcccacc
     2521 aaaactctct gccttgatag ccagaacaca caaagaccgt tgccggaatc tcatactttt
     2581 ccaatacggg tttggcttcg taaagattgt cggcatagcc gtcgtcgaac gtcacggcta
     2641 cagaactcgg cggtaccgca cccaccttca aacactgcac aagcttcgca agacggatcg
     2701 gacgggtgta cttgcgcaag gcatacattt gctgctcaaa atgttctgga gatacacaaa
     2761 cttcatagtc atcactctct acctctgcaa ccctgtgata ccccaatatc agagcccctc
     2821 catagatgcg agcttgtacc cagcgtgaga atgatttcgc cgtttttacg ccggggagtt
     2881 tcacttcgga tattccggtt tgaccgctcg gacagcaatt aggatctcgt aactgcgatc
     2941 cacaacatcg agttccttgg ttttcaaatc ttccacagat aaaccttcga gaaatgaaat
     3001 ggccgccaag acgtttccat atgaacggac ggtgacattc ccaactgaaa agacctcctc
     3061 gaataacaat cgtgcggatt ttgaggtgaa gcgccaatat tcaccccatt tgtcgaagtc
     3121 ttccaggctg agttgcgcca tgcccgaagc tgttgcgaga agaaccccac cgggtttaag
     3181 tagacggtac aaggtggcta tcgtcgtgcg aatgtcatag attacttgta aggtctgggt
     3241 aaagatgatc gcgtcgaaca gatcagacgg caaatggtct gctttcgtca ggtctgcgac
     3301 gatggtggca tcggggttgc cttccacagg atgaagaacg tcactccgag tgacacggtc
     3361 tccaccgtat ctgcgagtgt acgcattatg cttgatctct agcacgcaac ctcgaatatc
     3421 gccggaaaat tcagttaaga acttctcaat gtaatagcgg tcaatctcca gaccgcggct
     3481 attcccataa tcagaaccga tcggtttgag gcggcgaagg tcaccgaagc gcacccagcc
     3541 aacgggaggc cagacagcca atcgggcgat gcggcgtcgg gtttcacgcg acaaataccg
     3601 gcgagcaagg aggcgaagtt gatttagcac gttgttgatc tctggatgtg ttcctatggc
     3661 attgaggctg gtgcgagttt cacaccataa ctgttttcgt gtgtacggta atacatgaac
     3721 caggtttcgt tttcctcgtc aagcaaaagg accacctgct ggacttcatc gaatttatag
     3781 acttctacag gctcactcac gatgtttggc gtttgcagtg ataccaaccg gacctcggtg
     3841 cgcttctcac gcacattatt caataccagg gcatacagat cgtttgtcaa tttgacatgt
     3901 ccggctgtgc cccaaactgg aattggtttg ccaccggtcg taactgctga ggattcatgg
     3961 tgcgactcat catgatttcc atacgccacg ccaagcaatc cgcttttgga tgtgccatta
     4021 gggatgtaaa aaacaaacca ctcttccgcg tcgtaaatgg ctgctacgga aaataattca
     4081 tcccctccac cccaaacagc acggtcgttg cgatttagta cctgcccaag atcggtaaac
     4141 ttgaatccat cgggcgagga ggcaatacgt acatctgcat tgatcgtagt ggcgctctct
     4201 tgagtattgg caccatagta gatgaaagtt tctccatttt tacccaatgt tacacctgag
     4261 gacacagctc cctcttcgcc agattgattt gggaaccatg tgaggactgg acttccggca
     4321 tactttgtaa agtgaatacc atcatagctg gttgccacgc caatcgcgcg ccatagaact
     4381 gtttcatcgt attcttcgcg gtaatcactg gagccttgat aatagagata gtatgtatca
     4441 tctttcttaa taactgaaaa ggcaaagcca ccccataagt aagaatccca ttctccctcc
     4501 gcgccggctt caaggataat cccctgatcc acccattcgg acggagcagg gagtctgacg
     4561 cttgaaatcg gccattcaag tggcggcgtt ttcggatccg gaagacaggc gctcattacg
     4621 agagtgcatg tgagagctat gtagaggtac aaatgaaatt tatggcgcat cttttttcag
     4681 tggcaggagg gagtcatcat acaaatgact cagcccgaac ctatccacaa ttcgaagtac
     4741 gttatttatt tgtgatgagg tcattttctt tctccatccg gagaggttgt cgtctccggt
     4801 caccagcggg cttgtcaggc gtgtggtttg cgagggccgg tcaaaattac gggttaatga
     4861 aacgccggcc tttttcccca gggaaataaa cactgaggtc atctcgacct gtgtttgcgt
     4921 gcataggtcc tcgtaataca caagtttgag ctcaccgggc tggaactgcc ttaacggcac
     4981 gacattactg acgctccata taatcgcgtg cttttcctcg aaggttttga cattttttat
     5041 caaatccaaa tatggtccaa gatgatcttc aatgagattg ggctgtgaaa ggaacggttc
     5101 aatatcgaga tccgttgccc agcccaattc cattctcgac gaaaccactg cacagggatg
     5161 ccgcagcaga aatatgatag gaatttcggg gaaattgtta tgcaaccact tgagtgaaag
     5221 attcgctcga atttccttta tgaggcggaa ctccgaagtg atccgttcgt tgtggcgatc
     5281 aacccagtga ttgcgaatct tacccatgaa taggttctcg gcaaaaatgt aaaacgcctg
     5341 atccttaaca cccgggcgca tatattgaaa ataattgaag ttgcgatatt caggaacaag
     5401 gtgtgggttg aacggttcaa ataaaatacg gcaatttatc tgtgatgcga tcaaatcacc
     5461 gagccaggtg gtgccgcttc gtgcagttcc agcaactaga atacttctgc ggatatccgg
     5521 atctggattg aaataaaaat ggctaccaaa gaattgaatg gttcgtctaa aaaatttcct
     5581 cgacaggaaa cttatttgct tcaaatcgac accttccacg gaaagttatc cggttggttg
     5641 gcattcggct caacaatcat ccagtgacaa aatccaaagc agaaacattt gtgctttaga
     5701 ctaccatcaa aacgcctgat acacaaacgg ctgttcaaaa tcccctcccg taacaataaa
     5761 tactaaaaat agcacaattg ccatgcacag agctttggcc atagggggcc aattcaaaaa
     5821 tacaaactca tcctcatacc gataatgcat gaaatccatt atcagagagc cgaatacgag
     5881 aggcaaaaga agaaacattc gacggtaccg gatggctaca tccgaccagt cgaacaatcc
     5941 ctgccatatt tgcaaagcta gaggtaaatc ccaatgaaaa gggacccagg caagcattgt
     6001 gagcaagaaa acaacagcga tcccggcccc ctgcctccag atcggctgat tctgcggagg
     6061 aaccgccggc cgccacaacg tagaaaaacg ttcaacaata aggtacgttc cgtgtaaacc
     6121 tccccagatc agcatattaa cattgaagcc atgccaaaat ccgctaatca gcattgtgat
     6181 cattggaggc aagatcaggt tcgcgagctt tcgatagctg gccctgacgt ggctcaattc
     6241 gcggctgaca ggaaaatata tgtaatcacg cagccactca gacagggtga tgtgccagcg
     6301 cttccagaat tcggtgaaat tgcgtgagaa gtaaggcgcc cgaaaattgg ctgagagggt
     6361 gataccgaaa aaaccgctga ttccacgaac aatgtctgtg tacccggcaa agtcattata
     6421 gagagtgaac gcatagaaga ccaaccagat taatagctcc ggaggggtat atttcgcggg
     6481 gtattcaaag acaccggggc gaaaggatgc cattaaagaa tcggcgataa ttagtttgcg
     6541 tgtcaatcct gtgaagatga gcaccatact ttgagccagg acgcgattgt taaccaaacg
     6601 cggtgttgtg agtttcggta gaaacgtgcg tgcccgttca attggaccag caagcagttt
     6661 cgggaagtac gctaaatata aggaaaaatt tacccaatca gactcagcat ccaattggcc
     6721 gcggtacaca tctaccaggt atgaaatggt ctgcaacgta tagtaggaaa gacccagcgg
     6781 aaccagaatt tctagcccac ccgctcgcgt agacagtccc agagccgcta taaactcttc
     6841 caattccggt agaaagaatt gagcggtgcg aaaaaagacc catacagaaa cattaagacc
     6901 gattcctaac cataatagtc cgcgttgacc tcgatcgtcg atccgtaacc gcagcgccag
     6961 aaggaaattg atgagagtta acaaggccaa tacaacggca aatgtccaat cccaggaaaa
     7021 gataaagata taggaaacga acaaaagcca gatgttttgg agtcggagat ccaatagata
     7081 gtagattgct actgacaaga tcacaaatgt aaaaaaaaag atggaagtaa ttgtcatttt
     7141 ttaccagtag gtcgcttaag gccggaacgt ctcgttattt ccctcgaggt ctgcccatcc
     7201 gatctctcga cctaaccatg tactgaatac ttctgctcca accctgttaa ggtgactgta
     7261 atccgcccaa ccgtgattgg ggatgaaatc cagaggttcg gtttgccaga atggaacatt
     7321 gtgcaatgct gccagttcgt taacgcgggc ggtaaatcgg tcgtaatctg atttgccgtt
     7381 accaaagaag aaatacagcc catcggctac aggcatttcg accagaagaa ccgaagttcc
     7441 cgattcgtcg tacgccatga tgcgttccat ggcatccaga ttttcgtgca gaatctgata
     7501 aggcgagtaa atacggctgt agtatgtgac ttcaaacgaa tcatcatctg gtttcggagg
     7561 atcattaata taaatgccgg ttgtgttgag cggcgtaaag ccattgggaa gaatttcgta
     7621 gttcagtttc gtatcggacc aaattgtgtt ttcatactga aagcgggcga gtcggctgag
     7681 gtgttggcga taccgatata aataggaatt ttcctgcaac cagccctcaa atgaaaaatc
     7741 atcctgccga taggcgaccc aaggggtgtt cagaactacg gccggatcac gatcccctat
     7801 ggggactgca taatctcgtg catctgtgcc gacgatgaga agccgcgggt gataatcttc
     7861 gacgataatc cttgccaggg cagacgtgct ggctgctgta gacgcatcga tgccaaaatt
     7921 gaagcagtga atatctcgtc cggttattgc cttatattca gtttcgaatg ccgccggact
     7981 aaagcccgta tccaccatcg aacttcccag catgatacag tcaacgtcgc ccactttttg
     8041 agccaggcta tccagtaaag ccaatttatg tcccacttga taatgacgtg aacccatttt
     8101 aggtggagtc agtggtgctt gaaatgatgc agaacgagca gccgactcag ccaaagcaac
     8161 aagcaaaata aaaaaaagta cggtatagag tgcggttaat ccaggtactc ttcgtagttg
     8221 taaggtggag gatctcaatg caact
//
LOCUS       DASTJP010000079         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_986549, whole genome shotgun sequence.
ACCESSION   DASTJP010000079 DASTJP010000000
VERSION     DASTJP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986549"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..464
                     /locus_tag="VFQ23_02555"
     CDS             45..464
                     /locus_tag="VFQ23_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905485.1"
                     /translation="MKRFILRILFGSLMILSIVVTTAAAPLPVTSFTLVQGLPSTMNV
                     GDTFTVIVQVESDVEFSSVQALPSFMFPGKGVAAVQGGDRAGRGTSATVEVTFQAKSS
                     TANFPGGGVAPVHVVVGVRYGGGYVAVQDYLFNVAVP"
     gene            complement(552..1061)
                     /locus_tag="VFQ23_02560"
     CDS             complement(552..1061)
                     /locus_tag="VFQ23_02560"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET9905486.1"
                     /translation="MTPPPTWALALIYWLHMLATAVWVGSIAAISILILPAMKRTLSP
                     ETQLVFIEAIQKRLEPIAWFSISLLAVTGLFQMSTNAHYDGFLSISTQWSLAILTKHI
                     LGIFMIVASAIQTWEVIPAIHRAIMMSKKSKNTDEFHSLQRREILLLRINFGLAILIL
                     GASAVARVS"
     gene            complement(1058..2149)
                     /locus_tag="VFQ23_02565"
     CDS             complement(1058..2149)
                     /locus_tag="VFQ23_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905487.1"
                     /translation="MSENLGSTQPNIKAPKSTPSKSLRGRTFLFNAIGLIVFLSLAVL
                     AGYTSGIGTRRNTQSSVLAQQLSEQFQFALVDIQFGRYSNARQRLEFIIASDPSYPGA
                     QQKLTEVLVLINIPTPTLTPVPTTTPDFTGAEQGFASAQQLIAAQDWTGAIKALDEMR
                     KLDPNYQTSLVDGMYYFALRNYGYTLITSQGNLEGGIYQLTLAERFGPLDRDSNGLRE
                     GARFYLIGASFWELDWEQAYTYFDQVYRGWSGLWDGTMTAAERYHFAAMRFGDQLLTD
                     GQACDAVSYYEVAQALAPLDALAQEGYEAAYVTCNPPTEAVTATLTTPITVTPGGPTE
                     TPTETQPGPPPVTDTLAPPTETPSLTPTP"
     gene            complement(2175..>2371)
                     /locus_tag="VFQ23_02570"
     CDS             complement(2175..>2371)
                     /locus_tag="VFQ23_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905488.1"
                     /translation="HEPLNIWSSVGRGLVAAVIGGVVAYLVAVYLPGSAILTALLGMI
                     IGGLICIPIIWNELKLLLRL"
BASE COUNT          582 a          553 c          619 g          617 t
ORIGIN      
        1 tcgagtaaac tgacaacatc atattctcga aaggtttact tcacatgaaa cgatttatct
       61 tacggatctt atttggctcg cttatgatat taagcattgt cgtcacaact gcggccgctc
      121 ctctgccagt cacttcgttc acgctcgttc aagggttgcc ctccacgatg aacgtgggtg
      181 acaccttcac ggttatcgtt caggttgaaa gtgatgtaga gtttagttct gtgcaggcct
      241 taccctcctt tatgtttcct ggaaaaggtg tggcagctgt gcagggaggt gaccgcgccg
      301 gacgcggcac atctgctacc gtcgaagtga ccttccaggc gaagagctct acagcgaatt
      361 tcccaggcgg tggagttgca cctgtgcatg ttgttgtcgg ggtgcgttat ggcggaggtt
      421 atgtggcagt tcaagattat ttgttcaatg tagctgtacc gtaacgatct tttaacctta
      481 aatgaaaaca gcgtgccaaa gaggcacgct gtttttgtat agccgggtgg cgcccatgat
      541 tgaagggtaa tctatgagac gcgcgcgacc gcagatgcac caagaatcag gattgctaat
      601 ccgaaattga ttcgcaaaag caaaatttca cgccgttgta gagaatgaaa ttcgtccgtg
      661 tttttgcttt tcttcgacat catgatggcg cgatgaatgg cggggatgac ttcccaggtt
      721 tggatggcac ttgctacgat catgaagatc ccgaggatat gtttggtgag gatggctaat
      781 gaccactgcg tgctgatgga aaggaaacca tcgtaatggg catttgtact catttggaat
      841 aagcctgtca ccgccaataa actgatactg aaccatgcga tcggttcgag ccgtttttgt
      901 atcgcttcaa taaacaccaa ttgtgtttca gggcttagtg ttcgcttcat tgccggcaaa
      961 atgagtattg agattgccgc aatgcttcct acccaaacag cagtagcaag catatgcagc
     1021 cagtagatga gcgcgagggc ccaggttggc ggaggtgtca tggagtcggt gtaagtgaag
     1081 gcgtctcggt cggtggggca agcgtatcgg tgacgggagg aggtccgggc tgtgtttctg
     1141 tcggcgtttc ggtggggcct cccggagtta ctgtaatagg tgtggttagt gtggcggtaa
     1201 cagcttccgt cggtgggttg caggtgacat aggctgcttc gtagccctcc tgcgctaaag
     1261 cgtccaatgg agcaagcgcc tgtgcaacct catagtagga gaccgcatca caggcttgtc
     1321 cgtctgtaag caattgatct ccaaaacgca ttgccgcaaa atgatacctt tcagcggcgg
     1381 tcatcgttcc atcccataga ccagaccagc cccgataaac ctgatcgaaa tacgtgtaag
     1441 cctgttccca atccaattcc cagaacgatg cgccaattag ataaaagcgt gccccttcgc
     1501 gcagaccatt tgaatctctg tccagtggac caaaacgttc tgccagtgtg agctgataaa
     1561 tcccgccttc caaatttccc tggctggtga tcagggtgta gccataattc cgcaaagcaa
     1621 aataatacat accatcgact agtgaggttt gataatttgg atccagcttg cgcatctcgt
     1681 ccagagcctt gatagcgcca gtccaatcct gggcggcaat caattgttgg gcactggcaa
     1741 agccctgttc ggcacctgtg aaatcaggag tcgttgtagg aacaggcgtc agcgttggtg
     1801 tcgggatatt gatcagtact aagacttcgg ttagtttttg ctgtgctccg ggatagctcg
     1861 gatcactggc aatgatgaat tcgagccttt gtcttgcatt tgaataccga ccgaactgaa
     1921 tatcgaccag cgcaaactga aattgttcgc tcagttgctg tgccagaacg gaagattggg
     1981 tatttcggcg agttccaatt ccagaggtat aaccggccaa aacagccagt gagaggaaca
     2041 cgatcagacc aatagcatta aataagaatg tgcgtccacg caggctttta gagggcgtgc
     2101 ttttaggcgc ttttatgttt ggttgtgtac tacctaaatt ttcagacatg ggcggtatta
     2161 tatcagcctt atttttacag tcttaacagc aatttaagtt cgttccaaat gatcgggata
     2221 cagatcagtc caccgataat catgccaagt aaagcggtta atatcgctga gccgggtaga
     2281 tatacggcca ccaagtaagc tacgacccct ccaatcaccg cagcgaccaa gcctctgcca
     2341 actgaactcc aaatattcag cggctcgtgc a
//
LOCUS       DASTJP010000080         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_849081, whole genome shotgun sequence.
ACCESSION   DASTJP010000080 DASTJP010000000
VERSION     DASTJP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849081"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VFQ23_02575"
     CDS             complement(<1..1415)
                     /locus_tag="VFQ23_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9905489.1"
                     /translation="METIYKTFLERFNRLTYSQKFNVITAIFVLPVIAFLPLLSDQNV
                     RIDHYGRKEFFGTLYLRPLWQLTEDLQTHEITSQKYRAGDSQVSDLEAAQESIEDDFR
                     TLRRLHDEYRENLPLGTEADELYNQWLELKTATQNANTEAIERLVPDLYRNIRELVAR
                     VGDISFLILDPDLDTYYMMDAVLLKLPENQAILFEIHQLEGTVTNPQSLSVNERAQFA
                     ILLSRLEANLGEIDRNIAVSLENNNNGRMSVLVSQPLQDYQANLQAFIDLINSEVANP
                     PATQISADTLNAIIENQYNEARGANQEFYTAASEALEAGITSRLNSMIVRLYAIGLIA
                     LFSVLGAFILGQTIMNAIGEPLVQLADATQRLAAGDMTTRIPVKNTDELGQVSNAFNQ
                     MAEELEKDKTTLTARALDLEMANQISEKRARELQAISEISRIISTEQRLETLLALITR
                     LVSERFDFYHVGVFLIDNARQ"
     gene            complement(1418..>3114)
                     /locus_tag="VFQ23_02580"
     CDS             complement(1418..>3114)
                     /locus_tag="VFQ23_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9905490.1"
                     /translation="RAPHFDDLEQDFRARFINGFSWIGMTLLIAVTIIISVTGFTDYT
                     VYVLIGLIVVFALTIFLLKKRLLNLSALVLVVLTWIGIFFQAYTADGIHDVIMIGFIA
                     TALFASILIGWWAGGITILASILAAWLLASFEINQLITPTVSTPFTFARDLTFILMII
                     SALIYFSTTNLQNAIARAKESEKKLYVSNENLHKLNLSLEDRIAESTAELVVTNRRNE
                     RRAKQFEAIAQVARVIATVQDMDSLLPRVTQVISQQFGFYHVGIFLWDDNREYAVLMA
                     ANSEGGQRMLRRGHKLGIGQTGIVGFVAATGASRVALDVGADAVYFSNPDLPNTRSEL
                     ALPLRVANEVIGVLDVQSTEQNVFQEEDTEVLATLADQVAIAIQNGHSYEKTQQLLKE
                     AQKTSGEYLRDSWRILQSREEYIGYLMSDNTLKPLNRPVTSDQIDKAISTREHVAESG
                     KTATLAVPIRLRDEVIGIIDIHTTDQHIWDSDEVDIAEAVAERLSLALETSLLLQTTQ
                     RRAQIERATSEISGKISSSTQFDSILRTAAEELSRILGGSEVLVQIQPGSIVEEAN"
BASE COUNT          780 a          746 c          757 g          831 t
ORIGIN      
        1 aattggcgag cattgtcgat taagaagaca ccaacgtgat agaaatcaaa tctttcgctg
       61 accaaacgtg taatcagcgc gagcaacgtt tcaagtctct gctcggtgga aatgatacgc
      121 gagatttcac taatagcctg cagttcccgg gcacgctttt cactgatttg atttgccatc
      181 tcaagatcaa gggcgcgtgc ggtaagggtt gtcttatctt tttccaattc ctctgccatt
      241 tggttgaagg catttgagac ctgtccgagc tcgtccgtgt tcttcaccgg gatacgggtt
      301 gtcatatcac cggcggccag gcgttgtgtt gcatccgcca gctgaaccag cggttcgccg
      361 attgcgttca tgatggtttg ccccaagatg aacgctccaa gcacactgaa caaggcaatc
      421 agcccaatcg catacaaccg cacaatcatt gaatttaaac gtgatgtaat tcctgcctcg
      481 agtgcttccg atgctgcagt atagaattct tggttggcgc cgcgtgcctc gttatattgg
      541 ttctcaataa ttgcgttcaa ggtatcagcc gaaatttgag tcgccggggg attggctacc
      601 tcggaattga tcaggtctat gaatgcctgc aggtttgctt gatagtcttg cagtggctga
      661 gaaaccaata cactcatcct gccgttgtta ttattttcca acgatacagc aatattgcga
      721 tctatctctc cgagattggc ttcgaggcgg ctaagaagaa tcgcaaattg ggcccgctca
      781 ttaacagaca aggattgagg gttggtaact gttccttcca attggtggat ctcgaataat
      841 atcgcctggt tttcgggaag ctttagaagg acagcgtcca tcatgtagta ggtatccagg
      901 tcggggtcaa gaatgagaaa ggaaatatct ccgacccgcg cgaccaattc ccggatgttt
      961 ctatataaat cgggcaccag cctttcgatg gcttctgtat tggcgttctg ggtcgcggtt
     1021 tttagctcca gccattggtt gtaaagctca tcagcttctg tgcctagcgg gagattctca
     1081 cgatactcgt cgtgaagtct tctcaaggtt ctgaaatcgt cttctatgga ctcctgcgcc
     1141 gcttcaaggt cagaaacctg tgaatctccg gctcgatatt tttggctcgt aatttcgtgt
     1201 gtttggaggt cttccgttag ttgccagaga ggccgaagat acagggtgcc aaaaaactct
     1261 ttgcgcccgt aatgatcaat acgcacgttt tggtcagaca aaagcggtaa gaatgcaatt
     1321 actggaagaa caaaaattgc agtaattaca ttgaactttt gcgaataggt gagccgattg
     1381 aagcgctcaa ggaatgtctt ataaatcgtt tccataatta atttgcttct tcaacgattg
     1441 aacccggctg gatctgcaca agaacctcgg agccaccaag gatgcggctt agttcttctg
     1501 cggctgttcg caatatggaa tcaaactgag tggaggaact aatcttgccg gatatctcag
     1561 acgtggcgcg ctcgatctgg gcacgccgtt gcgtagtttg caggagcaag gaggtttcca
     1621 gcgccaacga caggcgttcc gccacagcct ctgcgatatc cacttcatcg gaatcccaaa
     1681 tatgttggtc ggtcgtatga atatcaataa tgccaattac ttcgtcgcgt aatcgaattg
     1741 gaacggccag tgtggccgtt tttccacttt cggcaacgtg ttctctggtt gaaatggctt
     1801 tgtcgatctg gtcggatgtc acgggccggt ttagtggctt caatgtattg tcagacatta
     1861 aatatccaat gtattcttcg cgtgactgta aaattcgcca ggaatcgcgc aaatattcac
     1921 cagaggtctt ttgcgcctct tttagtagct gttgggtctt ttcgtacgaa tgcccatttt
     1981 gaattgcgat cgcgacctgg tctgcaagag tagctaacac ctccgtatct tcctcctgga
     2041 acacattttg ttctgtgctt tggacatcca gcacaccgat cacttcattt gccacgcgaa
     2101 gagggagcgc cagctcagag cgagtattgg gtagatcagg attgctaaag tacacggcgt
     2161 cggcgccaac atccagggca acgcgcgatg cgccggttgc agcaacaaag cccacgatcc
     2221 ctgtttgccc aatgccaagt ttgtgaccgc gtcttagcat tcgctgaccg ccctcactgt
     2281 tggcggccat gagaaccgca tactctcgat tgtcgtccca caggaatatc ccaacatgat
     2341 agaaaccgaa ttgctggctg ataacctggg taacgcgagg caagagggag tccatatcct
     2401 gtactgtggc tatgactcgc gcaacctggg caatggcctc aaattgcttt gcccggcgct
     2461 cattgcgtcg atttgttact acaagctccg ccgtactctc cgcaattcga tcttcaagcg
     2521 aaaggtttag tttatgaaga ttctcattgc tcacatacag ctttttttca ctttccttcg
     2581 ctcgggcgat cgcattctgt aaatttgtgg tgctgaaata gatcaacgct gaaatgatca
     2641 tcaaaatgaa ggtcaaatcg cgggcaaacg taaaaggagt agagacagtg ggagtgatta
     2701 attgatttat ctcgaaactt gccagtaacc acgcagccag aatgctggca agaatggtga
     2761 tgcccccggc ccaccagcct attaagatac tcgcaaaaag tgcagtcgcg atgaagccga
     2821 tcatgattac atcgtgtata ccatcggctg tgtaggcctg aaaaaatatt ccaatccagg
     2881 tcaatacaac cagtacaagg gcgctgagat ttaacaatct ttttttaagc aagaaaattg
     2941 tcagtgcgaa tacgactatc agcccgatca aaacgtacac ggtgtaatcc gtaaagccgg
     3001 tcactgagat aataatagtg acggcgatga gtagcgtcat tcctatccag gaaaaaccgt
     3061 taatgaatct cgcgcgaaaa tcctgctcca aatcatcaaa gtgcggcgcg cgca
//
LOCUS       DASTJP010000081         7122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1480212, whole genome shotgun sequence.
ACCESSION   DASTJP010000081 DASTJP010000000
VERSION     DASTJP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480212"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VFQ23_02585"
     CDS             <1..1177
                     /locus_tag="VFQ23_02585"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9905491.1"
                     /translation="IFYVLAGCMPFDVVQQQQASTQVAETVANILTQTALSSPTPTLT
                     PFSPASVTPTLSPTSTSTATGTPTSIPVPCNRARFLGDVTIPDGTLLYPETPFTKIWR
                     LQNTGSCSWTTSYSLVFYSGDLMDGATVVSLPYNVNPGDMIDISIRLTTPQRAGTYQG
                     NWMLRDSNGVLFGVGTTANQAIWTRIVVRREAFFAVTSVVTSVDSFSYTGVCPATFTF
                     SAKVYTNGAGTVTYYWVRSDGSQSAEQTLTYTAAGAQTVTDTWNIGSPGAVINGWNKV
                     YINVPNHQNFGPITFRVFCYSPTSTPTVPPTKLPTETLTPRPTETVSPTATETATPTN
                     TGLPTGTATPSPTPIPSKAANPTEAQSPTPTVKPTLRLLDLCLASRIAFSSGCSDRL"
     gene            1249..1698
                     /locus_tag="VFQ23_02590"
     CDS             1249..1698
                     /locus_tag="VFQ23_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905492.1"
                     /translation="MKNQDVLTKGLAVVGTALVWIPILAPILFSAALWFAEKVWRIDY
                     LMPAELFLLSLTGGGLLIWATIRAHSHQKIIGWPLAIAVFLLVGGQVVAEVTGLASGA
                     VEPSGWKWGFILACLVGFSIALLTMGIGGVLLVHKLFKRDEFLHQLR"
     gene            1875..2918
                     /locus_tag="VFQ23_02595"
     CDS             1875..2918
                     /locus_tag="VFQ23_02595"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9905493.1"
                     /translation="MKQKFPALILAVSLIVTACSSSANANTATPEIIPTVIADHTLMA
                     EGRLEPIRYAEIAFNTSGVVNEVLVKEGERVQKGDLLIRLGDESDTNYAAAQLELVTA
                     QQALNDLLNTSEHELAQTIIDLKEAKEDYDKAVNYLHYLQNDQRVPQTDTRLILIQTW
                     KGYQYQYKSKNFKGPAPQDWIIEAENDLALKKAELDELQRAYDRMKNGTDTDQLAVLE
                     ARLDAAGARVAAFSVTAPFDSVVADLDATAGGSIHAGEIAVTVADFSKWVVKTTDLTE
                     LDVVNLADNQPAIITLDAFPDVELRGNVISIGQRYTEVQGDVVYEVTILLSEIHPDMR
                     WGMTASVTFIDED"
     gene            2992..4137
                     /locus_tag="VFQ23_02600"
     CDS             2992..4137
                     /locus_tag="VFQ23_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9905494.1"
                     /translation="MSILTNSQIVRAESGNGQSLIVDLRSVDKYYKSAAGDYHALKTV
                     DLQIKSGEFVSIIGKSGSGKSTLLNMIAGIDRPTTGEVFVNGTAVHEMNENQLAGWRG
                     DNLGIIFQFFQLLPALNLKQNVILPMDLAGKYRPRERRERADHLLEIVGLSDQMQKLP
                     SMVSGGQQQRAAMARALANDPPILIADEPTGNLDSRTAETVFTLFNQLVVEGKTVIIV
                     THDSSLARRAHRTALIADGEIVNEYIAKVLPSLTHPQLLAATHKLTPRHYEPGAMILV
                     EGKNSDAFYIISKGTVDVILPRSNQSDIIALQLGPGKYFGEMEFFHERRSRASIRACE
                     TSPVEVLALDYDTLSSLLNESEPTREALHLAADKHENENVEMRDGKG"
     gene            4164..6569
                     /locus_tag="VFQ23_02605"
     CDS             4164..6569
                     /locus_tag="VFQ23_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9905495.1"
                     /translation="MGVIWYKIWFDIWHNKTRTLLAVLSIAAGVFAVGAIFGMSQMML
                     SNMDRSHHEVLATHINAGLNAYVDRDTILNLRDIPGVEDVEPYNSVTVLYKLRPQDEW
                     RQGVIQMRDNYEQQKYELLQLRDGDWPRHKDEIAVERMGAQFLNIGIGDSVIFKIDEK
                     ERVLPITGFIRHPFVPPPQFQDLAFFFMSAEGMERFGVPPGKFSQLYVRVTPYSEEYA
                     KEVASAIKDKLAKQNISVGAFMYENPDKHWGRTFMDAMTQVQQLLALICVVIGAVLVF
                     NTISNLITQQTNQIGILKAIGGRAPSIVGMYLVSALVYGGLAFMIAFPLGAVVAHMIT
                     KVFLNLFNIDYDTFHISRDAVILQALSALLAPLLAGLPPILKGAAITVREAIASYGLG
                     GGYQSGRLDRIVESIGARLLPSHYATALGNMFRHKGRLLLTQLVLVAAGSSFLMVMSL
                     NTSLALTLDNFFARQHYDIFIQFDRDQRAARVITLAETVEGVEKAEIRIIQQAGMFVE
                     GQLLKEAGLSTTVWGIPSGSDFFKPLIVAGRWLAPEDGHALVITRDTAEKNNIHVGEK
                     VKLDLGVWGTDQWQIVGLYEPVFVGGFAADSIYAPQETLFRLAKKYNQADLILIRTTS
                     PERTFTTEVTKDLKETFERHKLDVSASQTQADLRATNEWQFSIVTSMLLALSIIVAIV
                     GAIALMGALSIGVIERTKEIGVLRAVGARSNTIMGIFVMEGVLQGLVSWLIAVPLSLL
                     VSPAAASQLGHILFGATLDYQYNWLAVGSWLGMVVIVSILASILPARGATRISVRDSL
                     AYA"
     gene            complement(6837..>7122)
                     /locus_tag="VFQ23_02610"
     CDS             complement(6837..>7122)
                     /locus_tag="VFQ23_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905496.1"
                     /translation="LDIVAVAENGELASFCTVWFDDVTRTAVFEPVGTHPNHQKRGLG
                     KAVMSEGLRRAQRLGATLATVSSYSTAAHALYESMGFTGVDLLEPWIREW"
BASE COUNT         1731 a         1843 c         1869 g         1679 t
ORIGIN      
        1 gatattttat gtgctggccg gttgcatgcc ttttgatgta gtgcaacagc agcaggcttc
       61 tacgcaggtt gccgagaccg tcgctaatat acttacccag accgccctct cctctcctac
      121 gccaactctc acgccgtttt cgcccgcctc agtgactcca acactgtcac ccacttctac
      181 aagcactgct acaggaacac ccacatccat tccagttccc tgtaatcgag cccgctttct
      241 cggtgacgta accatcccag acggtaccct actttacccg gaaactccat ttacaaaaat
      301 ctggagatta cagaatacag gctcgtgttc ctggacaacc agctactccc tggtctttta
      361 tagcggcgac ttgatggatg gtgcgaccgt ggtttccttg ccttataacg tcaaccccgg
      421 tgacatgatc gatatcagta tccgcctcac cactccgcaa cgcgccggaa cgtaccaggg
      481 caactggatg ctgcgcgact ccaatggagt tttgttcggc gtaggcacta cggccaatca
      541 ggcgatctgg acgcggatcg tggtccgccg cgaggcgttc tttgcggtga cgtctgtggt
      601 tacatcggtg gacagtttca gctacacagg ggtctgcccg gcaacgttca ctttcagcgc
      661 gaaggtctac acgaacggcg caggcacagt gacttattac tgggtacgca gcgatggctc
      721 gcagagtgca gaacagaccc tcacctacac agccgcaggc gcacagaccg taacggatac
      781 ctggaacatc gggtcgccgg gcgcggtaat caacggctgg aataaggtgt acatcaacgt
      841 acctaatcat cagaactttg gaccgatcac gttccgcgtg ttctgctact cgccaacttc
      901 cacgccaacg gtacccccca ccaaactccc tacggaaacg ctgactccaa ggcccactga
      961 gacagttagc cccacggcga cagaaaccgc cactccaaca aacaccgggt tgccaactgg
     1021 cacagctaca ccatcgccga caccgattcc cagcaaagct gcaaatccca ccgaagctca
     1081 gtctcctacg ccaactgtca aaccgaccct taggctgctc gatctttgtc tggccagtag
     1141 aatcgcattc tcctcagggt gttccgatcg actatagccg cttaataatg agtatgtttc
     1201 gaccgtccgt gaggttccag cacattctca ataatggagt ttttttacat gaaaaaccag
     1261 gatgttctta caaaaggttt ggctgtagtc gggaccgcgc tggtgtggat tcctatcctg
     1321 gcaccaattc tattctcggc agctctctgg tttgcagaaa aggtatggcg aatcgattat
     1381 ctgatgcctg cagagctctt cctgctgtct ttgaccggcg gcggcttact tatttgggcc
     1441 acaatccgag cgcattccca tcaaaaaata attggctggc cgcttgcgat agccgtgttc
     1501 ctgctggtgg gaggacaggt ggtcgcagag gtgacggggc tggcttcggg cgcagtcgaa
     1561 ccttctggct ggaaatgggg attcatcctt gcatgcctag ttggattttc aatagcactt
     1621 ctcacgatgg gcataggtgg tgtgctgctg gttcataagc tgttcaagag agacgagttc
     1681 ctgcaccaac tgaggtgatg acatggctca tggaagctct tgaaccaaca taatttttgg
     1741 caggacaata acatctgttt ctatgtgatt tgtctaccta tccacttgac cagtcaataa
     1801 ctcccccaca gataggagat tttttccgcg aaactcgtat gattcaatta aaaaaagctg
     1861 gaggtgtcat tattatgaaa caaaaattcc ctgccttaat cctggcggtc agcctgatcg
     1921 ttaccgcctg ttcatcttcg gccaatgcaa acaccgcgac acctgagata atccctacag
     1981 tgatcgcaga tcatacgctg atggctgaag gtcgcctgga gccgattcgc tatgcagaga
     2041 ttgccttcaa taccagcgga gtcgtaaatg aagtgctcgt gaaagaagga gagagggttc
     2101 aaaaaggaga tttgctcatt cgcctgggtg acgaatcaga tacgaactat gccgctgcac
     2161 agcttgagct ggtcacggcg caacaggcat tgaacgatct gctcaacacc tcagagcatg
     2221 aactggctca gaccatcatc gatctgaagg aagcaaagga agattacgat aaagcggtta
     2281 attatctgca ctacctgcaa aacgatcaga gagtacctca gacggatacc cgattgattc
     2341 ttatccaaac ctggaaaggg tatcaatacc agtacaaaag caagaacttt aagggtcccg
     2401 ccccacagga ttggatcatc gaggcagaaa acgatcttgc attgaagaaa gcagaactgg
     2461 atgaattgca gcgcgcttat gatcgcatga agaatggcac ggatactgac caattggctg
     2521 tgctggaggc acggctggat gccgcgggtg cacgagttgc cgccttctcc gtgaccgccc
     2581 cctttgacag cgtagtagcc gatctggatg caactgctgg tggttcgatc cacgccgggg
     2641 aaatcgcagt cacagtggct gatttttcca aatgggtggt caaaacaaca gatctcacag
     2701 agctggacgt ggtgaatctg gcagataatc agcctgccat catcaccctg gatgcctttc
     2761 cagatgttga attaagagga aatgtaatct ccatcgggca gcgctacaca gaagtccagg
     2821 gagatgtggt ctatgaagtg accatattat tatcagagat tcatcccgac atgcgctggg
     2881 gcatgactgc aagtgtgaca tttatagacg aagattaaat ggctgtctct tcggccttcg
     2941 ttgagatcta caatgcaatc aacgggaaga ggtggcgcct ggagtagccg gatgtcaatt
     3001 ctcactaata gtcaaattgt cagagctgaa agtggaaatg gtcaatcgct catcgttgat
     3061 ctgcggagtg tggataaata ctataagagc gcggccggag attatcatgc gctaaaaacg
     3121 gtagatctgc aaatcaagtc aggcgaattc gtcagcatca ttggcaaatc agggagtggt
     3181 aagtccacat tgctgaatat gatcgcaggc atcgaccggc ccacaaccgg cgaagtgttc
     3241 gtgaatggta cagccgtcca tgaaatgaac gagaatcaac tggcaggctg gcggggggat
     3301 aacctgggaa ttattttcca gttctttcaa ctattgccag cgctcaatct aaaacagaat
     3361 gtcatcctgc ccatggattt ggctggaaaa tacaggccgc gtgaacgccg ggagcgggct
     3421 gatcatttgc tcgaaatcgt tgggctgagc gatcaaatgc aaaaactgcc aagcatggtt
     3481 tccggtggac aacaacagcg tgcagccatg gcgcgtgcac ttgcgaacga cccgcccatt
     3541 ctcattgcag atgaaccgac cgggaatctt gactccagga ctgccgagac ggtttttact
     3601 ttattcaatc agcttgtggt ggaaggtaag accgtgatca ttgtcacaca cgacagcagc
     3661 ctggcgcgac gcgcgcatcg aaccgcgttg attgcagacg gtgagatcgt caatgaatat
     3721 attgcaaagg tgctgcccag cctcacgcac cctcagctcc tggccgccac ccacaaatta
     3781 acgccgcgtc actatgaacc cggcgcgatg attctcgtgg aagggaagaa cagcgacgcg
     3841 ttctacatta tttccaaagg gactgtggat gtgatcctgc cgcgctccaa tcaatcggat
     3901 atcattgcgc ttcaacttgg acccgggaag tacttcggcg agatggagtt cttccatgaa
     3961 cgtcgcagcc gggcatccat ccgtgcctgc gaaaccagcc ccgtcgaagt gctggcattg
     4021 gattacgata cgttgagctc actcctgaac gagtctgagc cgacgcgcga agcgctgcac
     4081 ttggcagccg acaaacatga aaatgaaaat gtcgaaatgc gggacgggaa gggataagtc
     4141 atccttcctt caggagtgcc aacatgggcg tcatctggta caagatctgg tttgatatct
     4201 ggcacaataa aacacgcacc ctcctggcag tgttgagcat cgcggccggc gtgtttgccg
     4261 taggggcgat cttcggcatg agccaaatga tgctttccaa catggatagg tcacaccatg
     4321 aggttttggc aacgcatatc aacgcgggct tgaatgccta tgtggatcgg gatactattc
     4381 tgaatctcag ggacattccc ggcgttgaag atgtggaacc ttataactcc gtgaccgttc
     4441 tctacaaact tcgccctcag gatgagtggc ggcagggtgt gatccagatg cgcgataact
     4501 atgagcagca aaaatatgaa ctgctccagt tgcgcgatgg cgattggccc cgtcataagg
     4561 atgaaattgc cgtggagcgg atgggcgcgc aattcctgaa tatagggatc ggcgattccg
     4621 tcatcttcaa gattgacgaa aaggaacgcg ttctgccgat tacggggttt attcgccatc
     4681 cttttgtgcc gccgcctcaa tttcaggatc tggcattttt cttcatgagc gccgaaggca
     4741 tggaacgttt cggagttcca cccggcaagt ttagtcaact ctacgtgcgt gtgactccgt
     4801 atagtgaaga gtatgcaaaa gaggttgctt ctgccatcaa ggataagctg gcgaaacaga
     4861 atatcagcgt tggggccttt atgtatgaga atcccgataa acactggggc agaacgttta
     4921 tggatgcgat gactcaggta cagcaattac tggctctcat ctgtgtcgtg atcggcgcgg
     4981 tgctggtctt caacacgatc tccaacctga tcacacagca aacgaaccag attggaatcc
     5041 tgaaagccat cggcggccgg gcgcctagca tcgtgggaat gtacctggtc agcgcccttg
     5101 tttatggcgg gctggcattt atgattgcat ttcctctggg cgcggtcgtc gcgcatatga
     5161 tcacaaaagt gttcctgaat ctattcaata tagattacga tacgtttcac atttcacgcg
     5221 atgccgtgat tcttcaagcg ctctcggcgc tgcttgcacc cttgcttgcg ggattgcctc
     5281 ccattttgaa aggagccgca atcacagtac gggaggcgat cgcaagctat ggcctgggag
     5341 gagggtatca gtcgggcagg ctggaccgga ttgtcgagtc gatcggggca cggttgctgc
     5401 cctcgcacta cgcgacagcg cttgggaata tgttccgtca caagggacgc ctgctgctca
     5461 cgcaattggt cctcgtggct gccggaagct ctttcctgat ggtgatgagt ctcaacacgt
     5521 cgcttgcctt aacccttgat aatttctttg cacgccagca ctacgatatc ttcatccagt
     5581 tcgaccgcga ccagagagcc gctcgtgtca ttaccctggc tgaaaccgtg gaaggcgtcg
     5641 agaaggcgga aatccgaatc atccagcagg caggtatgtt tgtggagggc caattgctta
     5701 aagaggcggg tctgagcact actgtatggg ggatcccatc tggcagcgat tttttcaagc
     5761 cgttgatcgt ggcagggcgc tggctcgcgc cagaggatgg ccacgccctt gtcatcacgc
     5821 gcgatacagc cgagaaaaat aacatccatg tgggcgaaaa ggtcaaactg gatcttggcg
     5881 tttggggtac ggatcagtgg caaatcgtcg ggctatatga accggttttt gtaggcggct
     5941 ttgcagccga ttcgatctat gctccccagg aaactctatt caggctcgcc aaaaaataca
     6001 atcaggcaga tctaatcctt attcgcacga catctccgga acgtactttt accacggagg
     6061 tgacgaagga tcttaaggaa acctttgaac gtcataagct tgacgtctct gccagccaga
     6121 cccaggccga tctgcgagct acgaatgagt ggcaattcag catcgttaca tcgatgcttc
     6181 tggctctttc gattattgtc gcgatcgtcg gtgcaatcgc gctaatgggc gccctatcca
     6241 tcggtgtgat cgagcgcacc aaggagatcg gcgtgctgcg cgccgttgga gcgcggtcga
     6301 acacgatcat ggggatcttt gtgatggaag gtgttttgca gggcttggtg agctggctga
     6361 ttgccgtgcc gctttccctt ctggtcagcc cggccgcggc ctctcaactg ggacatatcc
     6421 tgtttggcgc tacactcgat taccaataca actggctagc ggtggggagc tggcttggga
     6481 tggtcgtgat tgtttccatt ctggcttcga tcctgcctgc tcgcggagca acgcgaatta
     6541 gcgtgcgcga tagcctggcg tatgcatgaa caatgagtat ttcccaccag ccaagtaaca
     6601 atgtcactga gtaaaataca ttgagcataa cgatgttaag aaatgatcct catacgtcaa
     6661 cgcttgacga tatacaccaa ctgtcagatc gggtaattac agcccaggta gtttgaagat
     6721 aattcttctt gtcagcaatt catccaccct ttcacttcat tctatgtgaa taagtgaata
     6781 agcttcaatc gttatcagta cgtcttacga tatgttaatg tctccgaaag gaccggctac
     6841 cactctcgga tccaaggctc gagtagatct acgcccgtga agcccatcga ttcatagagt
     6901 gcgtgtgcgg ccgttgaata agaactgacg gttgccagtg ttgcgccaag ccgttgggcg
     6961 cggcgcaaac cttcgctcat gacagctttg ccaaggccgc gtttttggtg gttcggatgt
     7021 gtaccgacag gttcaaagac cgcggtacgg gtcacatcat cgaaccagac tgtgcagaac
     7081 gaggccaatt cgccgttctc ggcaaccgca acaatatcca gg
//
LOCUS       DASTJP010000082         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1514094, whole genome shotgun sequence.
ACCESSION   DASTJP010000082 DASTJP010000000
VERSION     DASTJP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514094"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFQ23_02615"
     CDS             complement(<1..917)
                     /locus_tag="VFQ23_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905497.1"
                     /translation="MKKLRIGVILSDVNVPVWVARMLERIRDSGHAKLIVLSFIGDSS
                     ATQEDLAKQLSKLHHQLDKAIFRPDPNPLEQFDVRTILPGVPLLQADPSKWSFLLKFA
                     DLDIFINLSQDRLPDVLLNAARFGIWSLRGNDKRITTSTGIGWLDMQREGSLINCAVE
                     AQRGESVQVIASSVMAANRYSFTHNQKSFLWRASSLLPHAIKQLFLTGEEKFFGEAES
                     VESAVKVSKRASGEIVDLALKQLVGKVTNKMRKRGSEDRWILMAGIRSSEDTLSWDKL
                     KPIVPPPGAFWADPFIIHKDSQTYIFFEE"
     gene            complement(914..1681)
                     /locus_tag="VFQ23_02620"
     CDS             complement(914..1681)
                     /locus_tag="VFQ23_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905498.1"
                     /translation="MSNTRSDIENWWAVEAMIASYNHVYQVTYKTRYEVQGPIGISIY
                     EGAPIASFQYEFLALSTDSKAVMDAVLAYDIPNEKQYVIDVFHPLPSSPALKEQYTKF
                     AHEFVRTGPILGLELPSKVTMIPDNVHIATTLQQAETANENLTGEGERIHAPTLRDKH
                     IHSLYVKVDGEAVGWLQLVTVYPHVGYINQLYVLKAFRNRKLGTALVQRAQTYAVKLG
                     LKHMALIPSDMALPLYRRLGYRPLLYFSAFRPNAGFS"
     gene            complement(1762..>2499)
                     /locus_tag="VFQ23_02625"
     CDS             complement(1762..>2499)
                     /locus_tag="VFQ23_02625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905499.1"
                     /translation="AVHNGGLVIGAFDHGQIIGFVFGFPGLESTPDGPRPKHCSHMMG
                     IHPDYRDKGIGFALKRAQWQMVRHQGLDHITWTYDPLLSRNARLNIAKLGAVCNTYRR
                     SEYGDMRDGLNAGLPSDRFQVDWWINTRRVDRRLGKRARRPLKLENFSEADLQPFYSV
                     HYSTESWPRPPEHFSPLEGHLALAEIPSDFPALKDADFSLGRDWRFFSREVFETAFSE
                     GYIVTDFVFDQGRSFYVLSNGESTLEQ"
BASE COUNT          680 a          627 c          597 g          595 t
ORIGIN      
        1 aactcttcaa agaaaatata ggtttgcgaa tccttatgaa taataaatgg atcagcccaa
       61 aaggcacccg gtggcgggac gatgggctta agtttgtccc aactaagcgt atcttcactc
      121 gaacgaatac ccgccattaa aatccagcga tcctccgacc cgcgtttccg catcttgttt
      181 gttactttcc caaccaactg cttcagggcc agatcaacga tctcaccgga agcacgtttc
      241 gagaccttca ctgccgactc tactgattct gcctcaccaa aaaatttctc ttccccggtc
      301 agaaaaagct gcttgatcgc atgtggaagg agcgatgaag ccctccacaa aaatgatttc
      361 tgattatgcg taaacgagta acggttggca gccatgacgg atgatgcaat tacttgcact
      421 gactcgccgc gttgtgcttc gacggcacag ttaataagag agccctcacg ttgcatgtcc
      481 agccagccga tgccagtcga ggttgtgata cgtttatcat tgccgcgcag ggaccaaatt
      541 ccaaatcgtg ccgcattcag caggacgtcc ggaaggcgat cctggctcaa attaataaaa
      601 atatccaggt ctgcaaactt aagtaaaaag ctccatttgc tggggtccgc ctgcaacaga
      661 ggaactcccg ggagaatggt tcgaacatcg aattgttcca atggattcgg atccgggcgg
      721 aaaatggctt tatccagttg atgatgcagt ttagagagct gcttcgccag gtcttcctgc
      781 gtagcagagc tatcaccaat aaaagacagc acaatcaatt tggcatgacc cgaatcgcga
      841 atccgttcca gcatacgcgc cacccagacc ggtacgttca catctgagag aatgactcca
      901 atgcggagtt ttttcatgag aaacctgcgt tgggacgaaa tgcagagaaa tataacaacg
      961 gacgatatcc caatcgtcga taaagtggga gggccatatc tgatggaatc agggccatgt
     1021 gtttcaagcc aagcttaacg gcatatgtct gggcacgctg gacaagcgcg gtacctaact
     1081 tgcgattgcg aaaggctttg agaacataaa gttgattgat atatccgaca tgaggataaa
     1141 cagtcaccaa ctgaagccag ccgacagctt ctccatcaac ctttacatac aacgaatgga
     1201 tatgcttatc acgcagggtt ggggcatgaa tgcgttcacc ctcaccagtc aaattctcat
     1261 tggcagtctc tgcttgttgt agcgtggtgg cgatatgaac attgtctgga atcatggtca
     1321 ctttggaagg caactccaac cccagaatag ggcccgtacg aacaaattcg tgagcaaatt
     1381 ttgtatactg ttctttcaac gcagggctgg agggtaaggg atggaaaaca tcgattacat
     1441 attgcttttc gttagggata tcgtatgcca ataccgcatc catgactgct tttgagtccg
     1501 tgctgagggc gaggaactcg tattgaaacg atgcaatcgg cgcgccttca taaatggaga
     1561 tgccgatcgg tccctgtact tcgtatcgtg ttttgtaggt cacctggtag acatggttat
     1621 aggaagcgat catcgcctca accgcccacc agttctcaat gtcagatcgt gtgttgctca
     1681 taattttaag acgccccctc gcgctataag ttactgaatg aaaatgacag ttacgttatt
     1741 tcaagtccct tagcccatac tttattgttc caacgtactt tccccattgg agagcacata
     1801 aaaactgcgt ccctggtcaa aaacaaaatc agtcacaata tatccttccg aaaacgcagt
     1861 ttcaaaaact tcacgggaaa agaagcgcca atcgcgcccc agcgaaaagt cggcatcctt
     1921 caacgcgggg aaatctgatg ggatttcggc aagagcaaga tgtccttcca gcggtgagaa
     1981 atgctccggt ggacgaggcc aactctcagt actgtaatga acggaataga acggttgcag
     2041 gtccgcttct gaaaaattct ccagcttcaa cggacgacga gcgcgtttcc caagccttcg
     2101 atcgacgcgg cgggtattga tccaccaatc gacctggaag cgatccgatg gaagacccgc
     2161 gttcaagcca tcgcgcatat caccgtattc cgaccgccgg taggtgttgc agacggctcc
     2221 caatttggcg atattgagac gtgcatttcg gctgagcaat ggatcatagg tccaggtgat
     2281 gtgatccagt ccctgatggc gaaccatttg ccattgggca cgcttcaaag cgaagccgat
     2341 acctttatct cggtaatcgg gatgaatgcc catcatatgc gagcagtgtt tcggacgtgg
     2401 cccatctggc gttgactcaa ggccgggaaa accaaataca aatccaatga tttgaccatg
     2461 atcaaacgcg ccgatgacca gtccgccatt atgaactgc
//
LOCUS       DASTJP010000083         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_563304, whole genome shotgun sequence.
ACCESSION   DASTJP010000083 DASTJP010000000
VERSION     DASTJP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563304"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1579
                     /locus_tag="VFQ23_02630"
     CDS             <1..1579
                     /locus_tag="VFQ23_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905500.1"
                     /translation="LMSKNVNAGSATVNIVQGYAASQTIVVFRNRVESQNNTPTNTPT
                     GTITPPTPTPTGTLNTPTPTVTGTVTPPTSTPTGTITPPTPTLTGTVTPPTSTPTGSV
                     TPPVCTPVVVTANFSQVGIGNSIEGMGVVAPNLNIDADASPNATAVRIAQGSGPILYN
                     ATGSGTLVPNGGISAGGGFSDVVTQLAGQPHRYTFTLAPGVTASNFSLHMLDFGDLNP
                     TLSTNHSVTMTAYNASNIIITRHELSYITLAEILPGSSNIYGNLQITGDAHSGLPGQP
                     GNWTWNLFGSGISRVVLEFGAGYDPNLGFDSLSYTTECVLSCQAPVVPDFSQIAAGQS
                     VEGLGAVAPNLNIDADASPNATAVRIAQGSGPILYNATGSGTLVGNGGIAPSGGFSDR
                     VTQLAAQPHRYTFTFTPGVTVTNFVLQMLDFGDLNPTLSTNHLVTLTAYDSSNAIVTS
                     QQLSYTTLAELLPSSSNIYGNLQITGDAHSGVPGQPGNWTWNVSGNGIVRIVLEFGAG
                     YDPNLGFHLLSFCPQLP"
     gene            1696..>2508
                     /locus_tag="VFQ23_02635"
     CDS             1696..>2508
                     /locus_tag="VFQ23_02635"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9905501.1"
                     /translation="MDLDDLDRFKQLDTLNMLGEIDNLPKQLANAYELGMRLDLPAWK
                     DIKQVVIAGMGGSAIGADLLASYSSSLSRVPVAVHRDYGLPLFAAGQDTLVICSSHSG
                     NTEETLNAFDAAIKAQCRIAVVCTGGELARRAEANNIPYWTFEHAGQPRAAVGFSFGL
                     LLAMFQRLVLIPDQTSEMEDTLNAMNRIQKHLRADVITTKNPAKRYAGQLMGRWVTVM
                     GAGLLTTVARRWKGQLNEVAKAGANFEFLPEADHNTLAGTMNPQETLNAHTMT"
BASE COUNT          632 a          637 c          621 g          618 t
ORIGIN      
        1 cttgatgagc aagaatgtca atgccggtag cgcgacagtg aatattgttc aaggatatgc
       61 agccagccag acgatcgtgg tgttccgcaa tcgggttgag tcacagaata atactccgac
      121 aaatacccct acaggaacga ttacgcctcc tacgcctacc ccaactggga cgctaaatac
      181 tccgactcct accgtaacag gaacggtcac cccgccaacc tccaccccca caggaacgat
      241 cacgccccct acacctactc taacgggtac agtgactccg ccaacttcca ctcctactgg
      301 cagcgttacg cctccagttt gtacgcctgt ggtagttaca gccaatttta gccaagtcgg
      361 cattggtaat tccattgagg gcatgggagt cgttgcccct aatttgaata tcgatgcgga
      421 cgccagccca aatgcaacgg ctgtgagaat cgcacaaggc tctggtccca tactctataa
      481 cgcaaccggt tcaggtacat tagtacctaa tggtgggatt tcagctggtg gagggtttag
      541 tgacgtagta acccaactag caggacagcc gcatcggtat acctttacgt tggcgccagg
      601 tgtcacggcg agcaacttct ccttacacat gttggatttt ggagacttga acccgaccct
      661 tagtaccaat cactctgtca cgatgacagc ttacaacgcg agcaatatta ttattaccag
      721 acacgaacta agttacatca cactcgccga gattcttccg ggtagctcaa acatctatgg
      781 aaatctgcag attacaggag atgctcacag tggtcttccg ggtcagccag gaaactggac
      841 atggaatctg tttgggagcg ggatctcacg ggtcgttctc gagtttggtg caggctatga
      901 tccaaatctt ggtttcgaca gcctgtccta tacgacagag tgtgtcctat cctgtcaggc
      961 tccggtggta ccagatttca gccaaattgc cgctggccaa tcggtagaag gtctgggagc
     1021 ggtagcaccc aatttgaaca tcgatgcgga tgccagtccg aacgcaacag ctgtgagaat
     1081 cgcacaggga tctggtccca tactctataa tgcaacaggt tcaggcacat tggtcgggaa
     1141 tggtgggatt gctcctagtg gtgggttcag tgacagggta acccagttag cagcacaacc
     1201 acatcgttac actttcacat ttaccccagg ggtgaccgtg accaactttg tgttgcagat
     1261 gttggatttt ggagatctaa atccgaccct tagtaccaat catttggtca cgctcacggc
     1321 gtatgactcc agcaatgcca ttgtcactag tcaacagctt agctacacaa ccctggcaga
     1381 gcttttgccg agctcgtcaa acatctatgg aaacctgcag attacaggag atgctcacag
     1441 tggcgtcccg gggcagcctg ggaactggac atggaatgtc tcaggaaatg gcatcgtcag
     1501 aatcgtcctg gaatttggcg caggctatga tcccaatctt ggcttccatc tgctttcgtt
     1561 ctgtcctcag cttccgtagc tgagcaagat acagaaaaaa tagcatgacc caccaccggg
     1621 tcatgctatt ttttttgtaa attttctttg aatttcaaac ctttactaaa atcgtagtaa
     1681 cagtataatt tcagtatgga tcttgacgac ctggatcgct ttaaacaact tgacacactc
     1741 aatatgttgg gtgaaatcga taaccttccg aagcaattag caaatgctta tgagctcgga
     1801 atgagactgg atttgcctgc ctggaaggat atcaaacagg ttgtgattgc ggggatgggc
     1861 ggatctgcca tcggcgccga tctgctggct tcgtatagtt cttcgctttc tcgagttccc
     1921 gtggcagttc accgcgatta tgggcttccc ctattcgcag ccgggcagga tacactggtg
     1981 atctgttcct ctcattccgg caacaccgaa gagactctaa atgctttcga tgcagcgatc
     2041 aaagcgcaat gccgaatcgc tgtcgtgtgt actggtgggg aattggcaag gcgtgcagaa
     2101 gcaaataata taccttattg gacgtttgaa cacgccggtc aaccacgcgc cgcagtgggt
     2161 ttctcatttg gtttacttct ggcaatgttc cagcgcctgg ttttgattcc ggatcagacc
     2221 agtgaaatgg aagataccct taatgccatg aatcgaattc agaagcattt gcgggcagat
     2281 gtcatcacga cgaagaatcc cgccaagcgt tatgcaggtc aactgatggg gaggtgggta
     2341 acggtaatgg gtgcaggatt acttacgacg gttgcccgtc gctggaaggg tcagttgaat
     2401 gaagttgcca aagccggcgc aaattttgaa ttccttcctg aagccgatca caatacgctt
     2461 gccgggacga tgaatccaca agagacgctc aacgcccaca caatgact
//
LOCUS       DASTJP010000084         4704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1302517, whole genome shotgun sequence.
ACCESSION   DASTJP010000084 DASTJP010000000
VERSION     DASTJP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302517"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFQ23_02640"
     CDS             <1..891
                     /locus_tag="VFQ23_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905502.1"
                     /translation="QWIGVPVASCVQAWGVLPPVGGPPEWLSTPTESENLGTDVPYNY
                     LAAQLIRSGMVDVSACADGGLLPDGGASGCGMDAARGAVTQWQNQFDEVILNVAKDSG
                     VPAHLLKNIFAVESQFWPGAVFKTDIGLGQLTELGADTAFLWNPPFFHQFCPLVMDSA
                     ECGDGYLHLDDEQQKYMRLALIDAVNANCEECPLGVDLDRANFSIGIFAHTLLANCEQ
                     AGQLVENYTGGPAGRAASYEDLWKFSLVNYNAGPGCLGEALDLTVGRDQTLNWDNLSF
                     NLAPACQGAIDYVNQISAQQ"
     gene            1044..3029
                     /locus_tag="VFQ23_02645"
     CDS             1044..3029
                     /locus_tag="VFQ23_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9905503.1"
                     /translation="MLTLIEKILFAIATAISLYYTYRGVQRIAKNISSGQGKIDWSLA
                     WQRLGELISKVLFFKPVFRFRPGPSFFHAFVGWGFFVYLLINISDLIYAYTGFKILEN
                     TGIFGDVYRLLADLLGVGILIGLASLAFRRYVLRPPTLTTRESTLLHPKARTGITRDS
                     AIVTTTFFTHNLMRLLGESFHLAKQGQTDSWQPIISAVSGLWSGVDANVLTFGEHIAF
                     WLSIGAVVAFLPYFAYSKHIHLFFAPINFAVKPERKSIGQLKYINLDDQSIEQFGAAT
                     MKDLGWEQIMDSYACIQCFRCQEVCPAYNTGKVLSPAALEVNKRYHLNYDGGLDVPML
                     NLISEEAVWACTSCGACVDICPVGNEPMRDILDIRRNLTMMESKFPKQLENAFKGMER
                     NANPWNVSQADRMKWAEGLTVPTIEQNPEPEILWWVGCAPATDARAQKTAQAFAKILN
                     AAGVNYAVLGKNESCNGDSARRAGREDIFFGLATQNVEILNEVKPKRIVTTCPHCLHT
                     IKNEYPAFGGNYTVIHHTQLINELVGAGKIQMKLDGDMMKITYHDPCYLGRHNKVFDA
                     PRDDLKSAGLEIIEMPRNSAKSFCCGAGGAQMWKEEEPGSARVNATRFAEAKSTGANT
                     LAVGCPFCMTMLSDASKADGGTLQVMDVAELVAERMK"
     gene            3126..3497
                     /locus_tag="VFQ23_02650"
     CDS             3126..3497
                     /locus_tag="VFQ23_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9905504.1"
                     /translation="MNFKCFFMVLSAMMLAACSNTGTITPTLTPIEEQGQAVYNLRCA
                     QCHALTPDTVVIGPSLAGVATRAASRMPGYDTQAYLETSILIPDEYLVEGFTNTMPTN
                     FGKELTSEEFSALIAYLMTLK"
     gene            3518..>4704
                     /locus_tag="VFQ23_02655"
     CDS             3518..>4704
                     /locus_tag="VFQ23_02655"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9905505.1"
                     /translation="MKFWIKRILLLLGVLAVIGIFYAAFAPLPYDPVLPQEKWGAGAS
                     SVLPAHSGLQREFPSLNGETTPEKTELGRMLFFDPILSKGQDISCATCHNPSLGFSDG
                     LQTAQGLDGSSLQRNALSLWNAGYGTRFFWDGRVTSLEEQMDLPLHAENEMAGSDEET
                     IARLKANPAYVTLFESAYSNEEDPITIANVQSAIASFERTLITNNSPFDKYAAGQFDA
                     LTAQQRRGLNLFRSAATRCFECHAAPTFGSEDFFVTGVPDLPGQEHDAGRATVVEDGK
                     DGAFKAPTLRNIVLTAPYMHNGVFATLEEVIDFYARGGGRDQGISVDRHILPIELNPQ
                     EREDLIAFLYALTDESALPEVPDAVPSGLPVVEQVANPARELVRQFNVEVTESGSGPA
                     HEPT"
BASE COUNT         1157 a         1170 c         1233 g         1144 t
ORIGIN      
        1 caatggatcg gtgtgcctgt tgcgagctgt gtacaagcgt ggggtgtatt accgcccgta
       61 ggcgggccac cggagtggct cagtaccccg actgagagtg aaaatcttgg caccgatgtt
      121 ccctacaatt atctggctgc acaattgatt cgcagcggaa tggtggatgt aagcgcctgc
      181 gccgacggag gattgttgcc agatggggga gcttctggct gcggcatgga tgcagctcgc
      241 ggtgccgtca cccaatggca aaatcaattt gatgaagtca tcttgaatgt agcaaaggac
      301 tcgggcgtgc cagcacacct gctcaaaaat atcttcgccg tcgagagcca gttttggcca
      361 ggcgccgtat tcaaaacgga catcggtctg ggtcaactca cagaactggg agccgatacg
      421 gctttcttgt ggaaccctcc attttttcat caattctgcc cactggtgat ggactcggca
      481 gagtgtggcg atggatatct gcatttggat gacgaacaac agaaatatat gagactggcg
      541 ttgatcgacg cggtgaatgc aaattgtgaa gaatgccctc tgggcgttga cctcgatcgc
      601 gccaacttca gcattggcat ctttgcgcac acgttgctcg ccaattgcga acaggctggc
      661 cagttagttg aaaactatac aggtggccct gctggaaggg cagcatctta cgaggacctt
      721 tggaaatttt cgctggtaaa ctacaacgcg ggcccgggat gtttggggga agcactcgat
      781 ctgacggttg gcagagacca gacgctaaat tgggataatc tatcttttaa tcttgcacca
      841 gcgtgtcagg gggcgatcga ctacgtgaat caaattagcg cacagcaata aagtctgtca
      901 ttaaaatttt tcgatcattg ttgtggaggc gctgcctcca catttgtttt gtgctaaact
      961 aaagaacctc tcccgcccac ttcgcgggca cctccctaaa ttcaaagaat ttggggaggt
     1021 ttgcggtgag gtctggagat ttcatgctta cactcattga aaaaattctc ttcgcaattg
     1081 caacagccat ttcactgtac tacacgtatc gtggagtaca gcgcatcgcg aaaaatattt
     1141 caagtggaca agggaagatc gattggtcac tggcctggca aaggctggga gaactgatct
     1201 caaaggttct tttcttcaaa cctgttttcc gcttccggcc aggccccagc ttctttcacg
     1261 cattcgttgg atggggtttt ttcgtctact tattaattaa tatttcagat ttgatctacg
     1321 cctacacagg ctttaagatc cttgagaaca caggaatctt tggggatgta tatcgattac
     1381 tcgctgacct tctcggagtg gggattctta tcggcttagc atcactggcc ttccggcgct
     1441 atgttctcag gcctccgact ctgaccacac gcgagtcaac attacttcat ccgaaagcgc
     1501 gcaccggcat cacgcgtgac tcggccatcg ttacaaccac gttctttact cacaacctga
     1561 tgcgactact gggcgagtca tttcatttgg ccaaacaggg acaaaccgat agctggcaac
     1621 cgatcatttc agcggtgtca ggtttatggt ccggtgtgga tgcgaatgta ttaaccttcg
     1681 gtgaacatat agccttctgg ttatccattg gcgcggtggt ggcattcctt ccatacttcg
     1741 cttattccaa acacatccat ttgtttttcg ctcctattaa ctttgcggtt aagcctgaac
     1801 gaaaatccat cggacagttg aagtacatca atctcgacga tcagtcgatt gaacaattcg
     1861 gcgcagcgac catgaaagat ttaggctggg agcagatcat ggatagctat gcctgcatcc
     1921 agtgctttcg ctgtcaggaa gtatgccctg cctataacac aggcaaagta ctatctccag
     1981 ctgcgcttga ggtgaacaaa cgttatcacc tgaattacga cggcggcctg gatgtaccga
     2041 tgttgaattt gatctcagaa gaagccgtgt gggcgtgtac atcctgcggc gcctgcgtgg
     2101 acatttgccc ggtcggcaat gagccaatgc gtgatattct cgatatccgc cgcaatctga
     2161 cgatgatgga gagcaaattt ccaaagcagt tggagaacgc attcaaaggc atggagcgca
     2221 atgccaaccc atggaatgta tctcaggcag atcggatgaa gtgggctgaa gggctgaccg
     2281 tcccgacgat tgaacaaaac cccgaacctg aaatcctctg gtgggtcgga tgtgccccgg
     2341 ctaccgatgc gcgcgcacaa aagaccgcgc aggcctttgc caaaattctg aatgcagccg
     2401 gagtcaatta tgcagtattg gggaaaaacg aatcttgcaa cggtgactcg gcacggcgag
     2461 cgggccggga agatattttc tttgggcttg caacgcaaaa cgtggaaatt ctcaatgagg
     2521 tcaagccaaa acgcatcgta accacctgcc ctcattgcct gcacacaatc aaaaacgaat
     2581 atcctgcctt cggtgggaat tacacagtga tccatcatac gcaattaatt aatgagctgg
     2641 tcggcgccgg aaagattcaa atgaagctcg atggcgatat gatgaaaatc acctatcatg
     2701 acccatgcta tttgggccgc cataacaagg tttttgatgc gccgcgtgac gatctaaaat
     2761 ctgcggggtt ggaaatcatt gagatgccgc gcaattctgc caagtctttt tgttgtggcg
     2821 caggtggcgc acagatgtgg aaggaagaag agcctggctc tgcccgcgtc aatgccacgc
     2881 gatttgccga ggcaaagtcc accggcgcaa atacgctggc ggtcggttgt ccattctgta
     2941 tgaccatgtt gagtgacgcg tcgaaggctg atggtggaac cctacaggta atggatgtag
     3001 cagagttggt cgccgagcgg atgaaataat caggaggtcg gaaggtatcc cttcaaatcc
     3061 gccaagcaac cgcattgcag ggcgctgtat aattcatgaa ctttaatact aaggaatccc
     3121 cctgcatgaa cttcaaatgt ttttttatgg ttctctccgc catgatgctg gcggcatgtt
     3181 ccaacacggg aacaattaca ccaactctta ccccgattga agaacaagga caagctgtat
     3241 acaaccttcg atgtgcacaa tgtcatgcgc tcacgcccga caccgttgtg attggtccat
     3301 ctttagccgg agtagcaaca cgcgccgcat ctcgaatgcc aggttatgat acccaggcct
     3361 atcttgaaac atcgattctg atccctgacg agtatctggt ggaagggttt accaatacga
     3421 tgccaaccaa ctttggcaag gaacttacca gcgaagaatt ttctgcactg atcgcctatc
     3481 ttatgacgtt gaaatgatta tcttttgagg agaagatatg aaattctgga taaaacgcat
     3541 ccttctactt ttgggcgtgc tcgcagtaat aggaattttt tacgccgcgt tcgctcccct
     3601 gccgtatgac cctgtgctgc ctcaagaaaa atggggtgcg ggtgcaagca gtgtactccc
     3661 agcgcacagt ggcttacaac gcgagtttcc atcgcttaat ggcgaaacga cccctgaaaa
     3721 aactgaactg ggacgaatgc tattctttga cccgattctc tcgaagggtc aggatatttc
     3781 gtgtgcgacc tgccataacc caagccttgg atttagcgat ggcttgcaaa ccgcacaagg
     3841 gttagacggt agctctcttc agcgcaatgc gttaagcctg tggaatgccg gatacgggac
     3901 caggtttttc tgggatgggc gcgtcacctc cctggaggaa cagatggatt tgccgcttca
     3961 cgcggagaac gaaatggcgg gttccgatga agaaacgatc gcgcggctga aagcgaaccc
     4021 cgcttacgtt acgctgtttg aaagcgccta cagcaatgaa gaagacccca ttacgatagc
     4081 aaatgtgcaa tccgcgattg cctcctttga aagaacgctc atcacgaaca acagtccatt
     4141 cgacaagtat gccgcgggtc aattcgatgc gctcactgcc caacaacgcc gtgggctgaa
     4201 cctgttccgt tcggccgcga cgcgttgttt tgaatgtcac gccgcgccga cgttcgggtc
     4261 cgaggacttc tttgtaacgg gcgtccccga tttgccaggt caggaacatg atgcggggcg
     4321 agctaccgtt gtagaagatg gaaaggatgg cgcgttcaag gcgccaactt tgcgaaacat
     4381 tgtgttgact gcgccgtaca tgcacaatgg tgtcttcgca acactggaag aagtgatcga
     4441 tttctatgcc agaggcggcg gacgtgatca gggcatctca gttgacaggc acatcctccc
     4501 gattgaatta aaccctcagg agagagaaga tttgatcgcc tttctgtatg cgctcaccga
     4561 tgaaagtgcc ttaccggaag tgcccgatgc tgttccatcg ggcttgccag ttgttgaaca
     4621 ggtcgccaac ccggcgcgcg aactcgtcag gcagtttaat gttgaggtca cggagtcagg
     4681 ctcgggtccc gctcacgagc cgac
//
LOCUS       DASTJP010000085         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_774089, whole genome shotgun sequence.
ACCESSION   DASTJP010000085 DASTJP010000000
VERSION     DASTJP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774089"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFQ23_02660"
     CDS             <1..313
                     /locus_tag="VFQ23_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9905506.1"
                     /translation="VEFPIKAYFRRRRPFISIIQAIVIGKKPGTWSFPSGHSAAAFAG
                     AWLFNRKFPGWSPLTYLIAGQVAFSRVYLGDHYPGDVASGSLLGMLFAMLFRRLLGRK
                     G"
     gene            409..2163
                     /locus_tag="VFQ23_02665"
     CDS             409..2163
                     /locus_tag="VFQ23_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HET9905507.1"
                     /translation="MRQSMLTDEQAQFLREEKDALAEIRLALAEIDVPKEALDTLQEA
                     ILQLDELFLLVVVGEFNAGKSALVNALLGEKVLLEGATPTTSRVTLVKWGEKVAEQVV
                     DESFAIYTYPLPLLQELNIVDTPGTNAVIRQHERLTSDYVPRSDLVLFVTSADHPLTE
                     SERQFLERILAWGKKVVFALNKADIIESDAALNEIRSFVLKHASAVLGDAPEFFPVSA
                     RLAQRALAETDPEQRKQLRAASRIDDLDRYIVATLDDNTRLQLKFNNPLGVAENLVAQ
                     TTKGINDQSEDIKEDQKTAAALETTISDYERDLKSELTPRLAEVENILYRLEQRGLDF
                     FDTTMRLTNIQELMRGDKVRAEFEKKVLTDVPKQIEEQVQRLIDWLVQRDLQEWQRVM
                     TYVQRRQAINIDHIVGAGIEPREVRRRELIDTMGKTVKRVVETYDRDREASELAAHVE
                     TAVAQTALLEVGAVGLGALVTTLVLSSSLDITGILAAGTLAILGIFVIPYRRKQAKDT
                     FKEKMTDLRTRLLDTLTTQFGKESENVVARLKDGVMPYVRYVRSERERIEKSENVLSK
                     LRQRLSALRARSQAVIGK"
     gene            2345..3010
                     /locus_tag="VFQ23_02670"
     CDS             2345..3010
                     /locus_tag="VFQ23_02670"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9905508.1"
                     /translation="MLFKTGVDVYSSDNEKIGTLHRVVMDPKTKEVSHIVVKEGFLFT
                     EDKVVPMDLVGSVTDDRISLQGSKEHLDELPEFEETHYVRPHDITGHGDVTSDISDSV
                     YWYPPVDLTPGGFGRYPVAFFPQSLYVQKVEKNIPDGMIALEQGAKVISEDGEQVGTI
                     ETLITDPKEDRVTHAVVSSGLLLKERKIIPAHWIDSVSDEEVHLSVASRILERLPEYH
                     PEG"
     gene            complement(3099..3323)
                     /locus_tag="VFQ23_02675"
     CDS             complement(3099..3323)
                     /locus_tag="VFQ23_02675"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9905509.1"
                     /translation="MNNPTSTYTQQEVQTLLMQDQLTGDYGIEVLDNNGIITLTGVVP
                     SREVMERAEAIARDVDGVQGVINQLELGNF"
BASE COUNT          840 a          809 c          919 g          858 t
ORIGIN      
        1 ggtagagttc ccgatcaagg cttatttcag gcgtcgtcgt ccgtttattt ccatcatcca
       61 ggccattgtg atcggaaaga agccgggtac ctggtcattt ccttcaggtc attctgctgc
      121 agcatttgcc ggagcatggc tgttcaatcg taagttcccg ggttggagtc cgctcacgta
      181 tctgattgcc ggtcaggtgg cgttctcgcg cgtctatctt ggcgatcact atccgggaga
      241 tgtggcttca ggttctttgt tgggtatgct gtttgcgatg ctgtttcgca gattgctggg
      301 gcgaaaggga taataaacgc aggtgactgt caactacaag ttgacagtca cctttttgat
      361 tcactgtata ataaatcaac ttcaatcgtt aaatcctgag gagaactcat gcgtcaatcc
      421 atgctcaccg atgaacaagc ccaattcctg cgcgaagaaa aagacgcgct ggctgagatt
      481 cggcttgcat tggctgagat tgacgttcct aaagaggctc tcgatacttt gcaggaagcg
      541 attctacaat tggatgaatt gttcctgttg gtggtggtgg gggagttcaa tgccggtaag
      601 agcgcgctgg tcaatgcatt attaggtgag aaagttttgc tcgaaggtgc cacgccgaca
      661 acctcgcgcg tgactctggt caaatgggga gagaaggtcg ccgagcaggt ggtggatgaa
      721 agttttgcga tctacaccta tcctctgccg cttctccagg aactgaacat tgtcgataca
      781 cccggcacca atgctgtgat ccgccagcac gaacgtctca ccagcgatta tgtccctcgc
      841 agtgatcttg tcctatttgt gacctctgcc gatcaccccc tgacggaaag cgaacgccag
      901 ttccttgagc ggattctcgc ctggggcaag aaagtcgtct ttgcgctgaa caaagcggat
      961 atcatcgaaa gtgatgccgc cctgaacgaa atacggtcct ttgtgctcaa gcacgcgtcc
     1021 gcagtattgg gggacgcccc cgaattcttt cccgtttctg cgagattagc acaacgcgcc
     1081 ctcgctgaaa ctgaccctga acagcgcaaa caattacgcg ccgccagccg catcgatgac
     1141 ctggaccgtt atatcgttgc caccttagac gacaacacgc gactccagct caaattcaat
     1201 aaccccctgg gtgtagcaga gaatctcgta gctcaaacga cgaaaggcat taatgatcaa
     1261 tctgaggata tcaaagaaga tcagaaaacg gctgccgccc tggagacaac gattagcgat
     1321 tatgaacgcg atttgaaaag tgaactgacc ccgcgcctgg cagaagtgga aaatatccta
     1381 taccggctgg aacagcgcgg tcttgatttc ttcgatacta ctatgcgtct gacgaacatt
     1441 caggaattga tgcgcggcga caaagtgcgg gcggagtttg agaagaaagt gctcaccgac
     1501 gtcccgaaac agattgaaga gcaggtccaa cgcctcatcg actggctggt gcagcgagat
     1561 ctccaggaat ggcagcgtgt catgacctac gtgcagcggc gtcaggcgat caacattgat
     1621 cacatcgtcg gtgcaggaat cgaaccgcgt gaagtgcgac gccgtgaatt gattgatacc
     1681 atgggtaaga cggtgaagcg tgttgtcgaa acatacgatc gcgataggga agccagcgaa
     1741 ctggcggctc atgtggagac cgcggtcgca cagacggctt tactggaagt tggtgcagtg
     1801 gggctgggag ctttggtcac aaccctggtg ctgtcctcca gtctggatat tacgggtatc
     1861 ctggctgcgg gaacgctggc gattctaggc attttcgtga ttccttaccg gcgcaagcaa
     1921 gccaaggata ctttcaagga aaagatgacc gatctgcgca ccaggctgct cgacaccctc
     1981 acgactcaat ttggaaaaga gtccgaaaac gtagtcgcgc gtttgaaaga tggcgtaatg
     2041 ccttatgtgc gctacgtccg ttcagagcgg gagcggattg aaaaaagcga gaatgtctta
     2101 tcgaaattgc gacagcgcct ctccgcactg cgggcgcgca gtcaggcagt gattgggaaa
     2161 tagaatcaac agttagtggg ggtgcctctc tgacacgtgg ggaaatcctg tattgataat
     2221 ttctggagcg tgatttacaa ctgtattgaa ccacagatcc atctgtgtgt ttggacgtat
     2281 ggaatggtga atgagcctgc catacgttta tgcttgtgtt tgtattccaa aaggagcaga
     2341 gaaaatgtta ttcaaaactg gagttgacgt atattcaagc gataacgaaa aaattggcac
     2401 cctccatcgc gtggtgatgg acccgaaaac caaagaggtt tctcacatcg tcgtaaaaga
     2461 aggatttctt tttaccgagg ataaagttgt tccaatggat ctggtcggtt ctgtcacaga
     2521 cgaccgcatc tcattacaag gatccaaaga gcatttggat gaactcccgg aattcgaaga
     2581 gactcattac gtgcgtcctc atgacataac gggtcatggt gacgttactt cagatatttc
     2641 agacagtgtg tactggtatc cgccggttga tttaactccg ggtggttttg gaagatatcc
     2701 ggttgcgttc ttccctcagt ctctctacgt gcagaaagta gaaaaaaata ttccggatgg
     2761 gatgatcgcc ctcgaacagg gtgcaaaggt gatttctgag gatggtgaac aggtcgggac
     2821 aattgagact ctgatcacgg atccaaagga ggatcgtgtc acccatgcag tggtttccag
     2881 cggcttgtta ctaaaggaac gaaagattat tccagctcat tggattgaca gcgtgagcga
     2941 tgaagaggtt cacctctcgg ttgcatcccg tatcctggaa cgcttgccgg agtatcaccc
     3001 tgaaggctaa acgatttggt aagtgatcca ccagaatgtg atgaaaagag cctgcggttt
     3061 cacgcaggct cttttttgtt attgtttcgc gacgacagtt agaagttacc cagctcaagt
     3121 tggttgatta cgccctgcac accatccacg tctcgtgcaa tggcttcggc tcgttccatc
     3181 acctcgcgtg atggaaccac gccggtgagg gtaatgatcc cattgttatc caggacctcg
     3241 ataccataat ctcctgtgag ctgatcctgc ataagcaagg tctgtacttc ttgctgggtg
     3301 taggttgagg ttgggttgtt catgttttat cactcctttt cgattcgtta ctatgatatg
     3361 gcgcaggaat attttctatc ctgattgtga ctgcaattcc agccagcgca ggatatcttt
     3421 ccggcg
//
LOCUS       DASTJP010000086         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_667895, whole genome shotgun sequence.
ACCESSION   DASTJP010000086 DASTJP010000000
VERSION     DASTJP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667895"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFQ23_02680"
     CDS             complement(<1..875)
                     /locus_tag="VFQ23_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HET9905510.1"
                     /translation="MIENESAWELSSIQTQDHKTLKSSDTCVILHLYYPEMWEEIRSY
                     LSHLGGQFDLYVTIPYEVDISETTLKAEIPNARIYRCENRGRDIAPFLRVFSAISELN
                     YKYICKIHTKKSVHIGAGKEWQQDMMDKLLGSPETIANIKQAFDTHTTWGLIGPQGHV
                     VPHIYYWAQNSEKVLHLAHSIDIPTDNIEFSYVAGSMFWFRPQALNLLLNPNILTQSF
                     EPEQGQQDGTLAHAFERFFGMVSNYAGYKIAESDSREVKIIDQFSFHFGLLAQEFEKQ
                     VQAVQTQVQVVQTQV"
     gene            complement(925..2010)
                     /locus_tag="VFQ23_02685"
     CDS             complement(925..2010)
                     /locus_tag="VFQ23_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905511.1"
                     /translation="MKKLNNRLIVVLGMHRSGTSVITRGLQVLGVELGDNLMPAYEGN
                     NSKGFWEDVDINALNIEMLRFLKIDWHFVTPVQASDVDALCQNGYLQRAVDLLRKKTA
                     SRKVFGFKDPRVAKLFPFWKEVFTQGQMNVDYILVIRHPLSVCHSLSKRDGFDFEKSY
                     LLWLEHVIGSLVGTEGETRVIVDYDTFMQTPETELTRIAKRLQLSMNADDFRNFQLEF
                     LDRGLQHTVYQLEDLTHDSAVPPLVQEVYSSLLQVTAGNAVPGEPALRDKIEHWGLEF
                     SRMRSTLAFVDKLGLKISAMTAERTGLVAERKALLQEKTEWTQAFMVKEQTVQRLRAE
                     LGAIHQSRWWRWTQPLRNLFKRPEPPK"
BASE COUNT          499 a          512 c          487 g          561 t
ORIGIN      
        1 tggacctgcg tctgaacaac ctggacctgt gtctgaacag cctggacctg tttttcgaat
       61 tcctgtgcca ggagtccaaa atgaaacgag aactgatcga taatttttac ttcacgcgag
      121 tcactttccg cgatcttata accggcataa ttggagacca tgccaaaaaa acgttcaaaa
      181 gcgtgggcca gcgtgccgtc ctgttgcccc tgttcaggct cgaaagattg tgtgaggatg
      241 tttgggttca acagcagatt gagagcttgc ggtctgaacc aaaacatcga gccagctaca
      301 taggaaaact cgatattatc cgttggaata tcaatcgaat gtgccaggtg caggaccttt
      361 tcagaattct gagcccaata gtaaatatgg ggtactacat gtccctgcgg tccaattaag
      421 ccccaggtcg tatgtgtatc gaacgcctgc ttgatatttg caatggtctc aggcgaaccc
      481 agcagtttgt ccatcatatc ctgctgccat tctttcccgg caccaatgtg gacactcttc
      541 ttggtgtgga tcttgcagat atatttgtaa ttaagttcag aaattgctga gaagaccctc
      601 aggaacggtg caatatcacg tccacgattt tcacaacggt aaatccgggc gttgggaatc
      661 tctgctttta aggtggtttc tgagatatcc acctcataag ggatggtgac atacaggtca
      721 aactgcccgc ccaaatgtga gagataagat cgtatctcct cccacatctc gggataatac
      781 aagtgtaaga tcacacaggt atctgagctt ttaagtgtct tgtgatcctg ggtttgaatg
      841 gatgacaatt cccaggccga ttcgttctcg atcatctatt aacctcttcg cggctttgca
      901 tgcaataacg acgcttcccg gatctcattt gggcggctca ggccgcttga acaaatttct
      961 gagcggttgt gtccagcgcc accaccgtga ttgatgaatg gcaccaagct ctgccctgag
     1021 tctttgcact gtctgttctt ttaccatgaa tgcctgggtc cattccgttt tttcctggag
     1081 gagtgccttt cgttcagcaa ccagtccggt tcgctcggct gtcatcgccg atatttttag
     1141 ccctagcttg tcgacaaatg caagggttga tctcatacgg gaaaactcca gaccccagtg
     1201 ctcgattttg tcgcgtaatg ctggttcgcc cgggactgca ttgcccgctg tgacctgcaa
     1261 caacgatgaa tacacttcct gcaccagagg cggcactgcg ctgtcatgcg tcagatcttc
     1321 aagctgatac accgtatgct gtaacccgcg atcgagaaat tcaagctgga aattcctgaa
     1381 gtcgtcggca ttcatcgaaa gttgaagcct tttggcaatc ctggtcagct ctgtttccgg
     1441 ggtttgcata aaggtatcgt aatcgacgat cacccgggtt tccccttccg tgccaaccag
     1501 gctgccgatc acgtgctcga gccataacag ataacttttc tcaaaatcga aaccgtcccg
     1561 cttggacaaa gagtggcaga cgctgagggg atgtcgaatg accaggatgt aatcaacatt
     1621 catctgtccc tgggtaaaga cttctttcca aaagggaaaa agttttgcca ctcgcggatc
     1681 tttgaagcca aatacttttc tgctggcagt ttttttccga aggaggtcga cagcacgttg
     1741 aaggtatcca ttttggcata atgcgtcgac gtcagaagcc tgaaccggtg tgacaaaatg
     1801 ccagtcgatt ttgaggaaac gtaacatttc aatattgagc gcattgatat ctacgtcttc
     1861 ccaaaagcct ttggaattgt ttccctcata ggcgggcatc aggttgtcgc ccagttcaac
     1921 acccaggact tgcagccctc gggtgatcac actcgtgccg ctgcgatgca tacctaatac
     1981 tacaattaat ctgttgttga gctttttcat aggaactggt tctcttaatg agatacgcgt
     2041 caatgccttg cgcggtcag
//
LOCUS       DASTJP010000087         7183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_295685, whole genome shotgun sequence.
ACCESSION   DASTJP010000087 DASTJP010000000
VERSION     DASTJP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295685"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1273)
                     /locus_tag="VFQ23_02690"
     CDS             complement(158..1273)
                     /locus_tag="VFQ23_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905512.1"
                     /translation="MSALTFTLATPADDESLRRLLRKNPIRGSISLSFEREPCYFDAS
                     IVEGPFHQTIVAREVETGEIIAFGNRSIRPLYVNGEVKETGYMSQLRVRPDYGKGLYL
                     ARGLAGGFRKYHELHQDRRTPFYLMSVIEDNLPAKRLLTSGLAEYPYVQKYARFFTYA
                     IYPLFRKRRLSLPGALRLIRGSEKYIDGIVNCLNRNNSRKQLAPHWTPESLFIANLKP
                     SDFFLALDGERVVGCVACWDQSAFKQTVVRGYAGSFARWRKPLNMFSRLGGWPYLPEP
                     NTPLRYSHASHLAIDNDDQLIFQALLRAVYQHNLEYGYNYFMLGLAESNPLRNIVQEY
                     RPLTYASQLYLVTWDEGREAIAKVDHTRIPAPEIALL"
     gene            complement(1285..2700)
                     /locus_tag="VFQ23_02695"
     CDS             complement(1285..2700)
                     /locus_tag="VFQ23_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9905513.1"
                     /translation="MRLTLIHPCIGRRIDQPYIRLWQMEPLPPAVIAGLTPSDVEIKF
                     YDDRMERIPFDEPTDVVGISVETYTAKRAYQIASEYRKRGVPVVMGGFQAALCPEEVS
                     QYADAVIVGEAEEVWEQVLLDVERGALKTYYQSNGRPSLQNLSPKRSIYQGKNYLPIG
                     LVEAGRGCHFVCDFCAVQTVFNHTQTRRPVDDILIELEKMRDKPLIFFVDDNITSNMD
                     QAKAFFRELAKLKIRWVSQASINAAHDEEFLQLIAASGCQGVLIGFESLNQDNLKKMN
                     KGFNTMKGGYEKALENLRKHNIRLYITFVFGYDEDRESSFSETVAFALNHNFYIAAFN
                     HLTPFPGTPLYRRLHQEGRLLYENWWLDDRYSYNMIPFQPAHMTPERLQRGCVEARAE
                     FYSYSNIWRRSFDSVNRSNSAMWWHFYGINYLFRREVRQRDYYPLGDESFKGRLLKVR
                     ERGEPLPQIVHTVDSQKTLTI"
     gene            complement(2697..3539)
                     /locus_tag="VFQ23_02700"
     CDS             complement(2697..3539)
                     /locus_tag="VFQ23_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9905514.1"
                     /translation="MQSIVQLEAHVAARPNSIEVNLALAEAYEEQGRWQEAAEVYRSL
                     LALYPATASLFVNRIRLGAIALGISSILILAALFMQVSLINSENNPREFATALASKEY
                     LFSQLFILIAFPFLSTAAISVYKLLSYSRDHRPAFWAMVFSVIGIGLSMPALGINAVI
                     LPLIGKLYLEGQLGVLAIYAALQEYPWSWILAIGSYLLLAGIMLFSWVIVRNKGFPKW
                     AAVFYLTGWALFVITSYPMPKFAMISIGVLILLGGIELARSLWVQATLQFTPAVDSIQ
                     RAES"
     gene            complement(3543..4919)
                     /locus_tag="VFQ23_02705"
     CDS             complement(3543..4919)
                     /locus_tag="VFQ23_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9905515.1"
                     /translation="MKLVLISPKGPLYRHRGGIFKRNLRYAPLTLTTLAAYIPPELNM
                     EVEIIDEGIENVDLNLTADLIGMTVITGSSNRAYELADHFRERGIPVVLGGPHITLIP
                     EDAEPHADSIVVGYAEDTWPELLRDFVAGEMKPRYTQSPGLSLANRPFPRRELMKAHK
                     FLTTHVFEATRACVHSCDFCVVPTAWGLKPFQKPVEEVVEDIKQHWAKKIIFIDLNLI
                     ADKAYAAKLFEALIPLKLNWFGLSTTLLGRDKSLLELAARSGCTGLLMGFESITPENL
                     KQSKKGFNSPAQYQDLVSLLHKYGITLMACFTFGMDNDTPEVFMKTAKFAIESGIDLP
                     RYAIVTPFPNTGLYKRLESEGRILTKNWELYDAQHVVFQPKMMTPQELYEGHEKAWKY
                     TYSYTAMARRYLKSRIQTPVWWVANMGYRFYAHHLRDFYNCDWILGQLEPQKPTSSKS
                     DLIQVTGD"
     gene            complement(5122..5823)
                     /locus_tag="VFQ23_02710"
     CDS             complement(5122..5823)
                     /locus_tag="VFQ23_02710"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9905516.1"
                     /translation="MYFVRKSILILLAGLLMVACSGQQTASEPTQDVNAILTAGVATF
                     AAAVFQTQTAVAPPATATPTVTNTPTATVATPIPLASPTASATATQLIFVAPLVTFTA
                     VITGTPPTPNPTTLASGCNNLLLLRDETVPAGTVFNPEEGFTKVWKVANSGSCDWVFF
                     YHLVFVSGERMGGEPGRLGKVIEPGKWTQLSLNLQAPRNPGTYTGYWRFADNSGTAFG
                     STLSVSIEVKASSYP"
     gene            6163..>7183
                     /locus_tag="VFQ23_02715"
     CDS             6163..>7183
                     /locus_tag="VFQ23_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905517.1"
                     /translation="MFNKLFRQAEIPAEYRSNFLHLYLDIAWFGVLSGSAINFLSIYA
                     ARIGASGFQIGMIGAMSALVSLFLAIPSGRWLQTRNTGRAIFWASVLYRIWYLPFIFL
                     PWLFDGQGQIWAIIAITFLMAIPLTPLGVGFNALFAEAVPIEYRAHVAGIRNIMLSIA
                     FMVTSLVSGTILERSTFPAGYQIIFAIGAFGAGMSSLHLYFVRPLLINRSTKDDGSPR
                     AASTSPSIKDRLYSRIIVTALRLDIWKTSFRKVLLCMLGFHLTQYLAIPIFPLYYVNE
                     LHLNDDHIGIGTALFYLTVLIGSTQLRNTVHRAGNKNVTAYGVIGMAIYPFLLALSAE
                     VWQFYG"
BASE COUNT         1826 a         1784 c         1758 g         1815 t
ORIGIN      
        1 acaatccatg tgccgcgcaa caaatacgtg aggctggtga tgttgcgcac gcgtgggttt
       61 tcccaaccgg tccgcgagcg aaagaatgga aagagcagga agatcagtcg atttcccaca
      121 ccaatgagca agtccaataa agcgtcaagg attctggtca tagtagagca atttccggcg
      181 cagggatacg tgtatgatcc acctttgcaa ttgcctctcg accttcatcc caggtaacaa
      241 gatagagctg gctagcgtag gttaaggggc ggtattcttg cactatgttc cgcaacggat
      301 ttgattcggc aagcccgagc atgaagtaat tgtatccata ctctagatta tgttgataga
      361 ctgcgcgcag aagtgcttgg aaaataagct gatcgtcatt gtcaatcgcc agatggctgg
      421 catgggaata tctcaagggt gtgtttggct ccgggagata aggccatccg ccaagacgcg
      481 aaaacatgtt cagtggttta cgccagcgcg caaaagaacc tgcatagccg cggaccacgg
      541 tctgtttgaa ggcagattga tcccaacagg cgacacatcc cactacgcgt tcgccatcga
      601 gtgcaaggaa aaagtcggag ggtttcaggt ttgcaatgaa cagtgattca ggagtccaat
      661 gaggggcaag ttgttttcgg gaattgtttc gattcagaca attcacgatt ccatcgatat
      721 atttttcact tccacgaatc agacgaagag cgccaggtaa tgatagccgt cgcttacgaa
      781 acaacggata aattgcatat gtaaaaaatc gagcatactt ttgtacgtac ggatattccg
      841 ctaagccaga ggtgagcagt cgctttgcgg gcaaattatc ttcaatcacg ctcatcagat
      901 aaaacggtgt acgtcggtcc tgatgaagct catgatattt ccggaacccg cctgccagcc
      961 cccttgcaag atataagcct ttgccataat cgggtctcac gcgcagctgg ctcatatagc
     1021 cggtctcttt cacttcgcca ttcacataaa gaggtcgtat ggaacgattc ccaaacgcaa
     1081 tgatctcacc cgtttccact tcacgcgcga cgatcgtctg gtgaaagggt ccctcgacaa
     1141 tggatgcatc gaaataacat ggctcacgtt caaaggacag cgagatcgac ccccgaatcg
     1201 gatttttccg caaaagccgc ctcaaggatt cgtcgtctgc cggtgtcgcc agcgtgaaag
     1261 taagagcaga catgcagccc tgagctagat ggtaagagtc ttttgtgaat caaccgtgtg
     1321 tacgatttga ggcagcggct cgcctcgctc ccggaccttg agcagacgtc ccttaaatga
     1381 ttcatcaccc agcggataat aatcccgctg acgtacctcg cggcggaaga gatagttgat
     1441 cccgtagaaa tgccaccaca tcgcggaatt ggagcggttg accgaatcaa agcttcggcg
     1501 ccagatgttg ctataactgt agaattctgc gcgcgcttct acacatccgc gctgtaggcg
     1561 ttcgggcgtc atatgtgcag gctgaaaggg aatcatgtta taggaatacc gatcatccag
     1621 ccaccagttt tcgtacagta agcgaccctc ctgatgtaac cgcctgtaca gcggagtccc
     1681 cgggaaaggc gtgaggtggt tgaaggcggc gatataaaaa ttgtgattca gcgcaaacgc
     1741 cacggtttca gaaaaactcg attcccgatc ttcgtcgtag ccaaagacaa aggtgatgta
     1801 aagacgaata ttatgttttc gaaggttttc aagagccttc tcatagcccc ctttcatcgt
     1861 gttgaatccc ttgttcatct tcttgagatt atcctgatta agagactcaa agccgatcag
     1921 cacaccctgg cagccgcttg cggcgatcaa ctgcaaaaat tcttcgtcat gcgcggcgtt
     1981 gatgctagcc tggctaaccc agcgaatctt aagtttggca agttcgcgaa agaaggcttt
     2041 tgcctgatcc atattggagg tgatgttatc gtcgacaaag aagataagcg gtttgtcgcg
     2101 catcttttca agctcaatca agatatcgtc tacggggcga cgtgtctgcg tgtggttgaa
     2161 aacagtctgc accgcgcaaa agtcacaaac gaagtgacaa ccgcgcccgg cttcgaccag
     2221 accaatcggc agataatttt ttccctgata aatcgaacgt ttcggactca agttctgaag
     2281 cgatgggcgt ccgttcgact gataataagt cttcagcgcg ccgcgctcta catccagcaa
     2341 aacctgttcc cacacttctt ccgcttcgcc aacgatcacg gcgtctgcat attgagacac
     2401 ttcttccggg cataacgcag cctggaaacc tcccatcacg acgggcaccc cgcgtttccg
     2461 gtactcgctg gcgatctggt aggcgcgttt ggcagtgtag gtctccacgc tgatgccgac
     2521 cacatccgtt ggttcgtcaa acggaattcg ctccatacgg tcgtcataga atttgatttc
     2581 cacatcgctc ggcgttagcc ctgcgatcac cgcgggaggc aatggttcca tttgccagag
     2641 acggatataa ggctggtcaa tgcgtcgtcc aatgcagggg tggattaggg ttaatctcat
     2701 gattcggctc tttgaatgga gtcaactgcc ggtgtaaatt ggagggtcgc ttgtacccaa
     2761 agactgcgtg caagttcaat tccccccagc aggataagca ctccaatcga gatcattgca
     2821 aatttcggca tgggataact tgtaatcaca aatagagccc agcccgttaa atagaaaacc
     2881 gctgcccatt ttggaaagcc tttattccgg acgatcaccc agctaaagag cataatcccg
     2941 gccagtaaca aatagctgcc aattgccagg atccatgacc acggatattc ctgcagcgcg
     3001 gcatagatgg ctaaaacacc aagttgccct tctaaataca acttaccaat taaaggaagg
     3061 atcaccgcat tgatcccaag cgctggcata gacaacccaa ttccgataac tgaaaaaacc
     3121 atcgcccaaa atgcagggcg atgatcgcgt gaatacgaca ataatttgta gaccgaaata
     3181 gcagccgtgg acaaaaaagg aaacgcaatt aagatgaata gttgcgagaa gagatattct
     3241 ttggaagcca aagcggttgc aaattcacgt ggattgtttt cagaatttat cagagaaacc
     3301 tgcatgaaga gagcagccag gattaagatc gaggaaatac ccaatgcaat cgcgccaagg
     3361 cgaattcgat tgacaaataa agaggccgtc gccggataca aagcaagaag tgatcggtat
     3421 acttctgccg cctcctgcca gcgcccctgc tcttcgtagg cttctgccag cgccagattg
     3481 acttcgatgc tgttcggtcg tgctgcgaca tgggcttcaa gctgaacaat agactgcatc
     3541 cgctaatctc ccgttacttg tatcaaatct gattttgagg aagttggttt ctgcggctct
     3601 aactgtccga ggatccagtc gcagttgtag aagtctcgca ggtgatgggc gtaaaatcga
     3661 taccccatat ttgccaccca ccagacgggc gtctggatac gcgatttgag atagcgccgc
     3721 gccatggctg tgtaggaata cgtgtacttc caggcttttt cgtggccttc gtacagttcc
     3781 tgcggcgtca tcatcttcgg ctggaagacg acatgctggg catcgtagag ttcccagttc
     3841 tttgtgagaa tgcgtccttc tgactcaagg cgcttgtata agccagtatt tggaaaaggc
     3901 gtgacgatgg catagcgcgg caggtcaatt ccggactcga tcgcgaattt cgccgttttc
     3961 atgaagactt ccggcgtatc gttatccatg ccaaaggtga agcaggccat gagggtgatg
     4021 ccatacttat gtagcaatga cacgaggtcc tggtattgag ccggagagtt aaatcccttt
     4081 ttcgattgtt tgagattttc gggcgttatc gattcaaacc ccatcaacaa gccggtgcat
     4141 ccgctccgcg cggcaagctc aagcaaagac ttatcccttc cgagaagggt cgtggaaaga
     4201 ccaaaccaat taagcttcag cggaatcaac gcctcaaaca atttcgcagc atatgcttta
     4261 tcagcaatca agttcaaatc aatgaagatg atcttttttg cccagtgctg tttgatatct
     4321 tctacaactt cttcgactgg cttttgaaat ggtttgagtc cccaggctgt tggcaccacg
     4381 cagaagtcgc agctgtgtac acaggcacgt gttgcttcaa agacgtgcgt ggtgagaaat
     4441 ttatgtgcct tcattagttc acgacgtggg aatggacgat tcgcaagact cagccctggg
     4501 ctttgtgtgt aacgtggctt catttccccg gcaacaaaat ctcgaagaag ttcgggccac
     4561 gtatcctctg cataccccac cacaattgag tccgcgtgag gctcggcatc ttccggaatg
     4621 agtgtaatgt gaggtccccc caacacgaca ggaatgccac gttctctgaa atgatctgct
     4681 aactcgtatg cacggttgga tgaaccagta ataaccgtca tgccgattag gtctgccgtc
     4741 aggttaagat caacgttctc aatgccttca tcgatgatct ccacctccat attaagttca
     4801 ggggggatat aggcagccag cgtagtaagt gtgagaggag catagcgcag gtttcgtttg
     4861 aaaatcccac cccgatgacg atacaagggg cctttcggag aaatcaggac aagtttcata
     4921 ggagatacct tctttcacac gaacaaggat aactaaactt caagccacag tattttgacc
     4981 cctacagatc gtccgtactt attaacccat tcccgtttcg caagtttcac ggttctgttc
     5041 ttttgcaacc ggcctcaatc attcaaattg aggccggaat taatcgcgta ttggtcggga
     5101 agtcatgaag agacagaccg cttacggata ggaagaggcc ttcacctcaa tcgaaacact
     5161 aagggtggac ccaaacgccg tccctgaatt atcagcgaat cgccagtagc ccgtatacgt
     5221 tcccgggttt ctcggtgcct gcaaattgag ggagagttgc gtccatttcc ccggctcgat
     5281 gacttttccg agccggccgg gttcgccgcc cattctttca ccgctgacaa aaaccaggtg
     5341 ataaaagaag acccagtcgc aagatccgct gtttgctacc ttccacacct tggtaaagcc
     5401 ttcctcgggg ttgaacactg ttccggcagg aactgtttca tcgcgcagga ggagtaagtt
     5461 gttacaccct gatgccagcg tagtaggatt gggtgttggg ggagttcctg taataacagc
     5521 agtgaacgtc accagaggcg cgacgaaaat caactgcgtt gctgttgctg atgcagtagg
     5581 tgaagccaga ggaataggtg tcgccaccgt tgcggttgga gtattggtaa cagttggcgt
     5641 tgcagtggca ggtggagcga ccgccgtttg agtctggaag accgccgctg cgaatgtcgc
     5701 cactcctgcc gttagaattg cgtttacgtc ctgagttggc tcagacgcag tttgttgacc
     5761 ggaacaggct accatcagaa gccccgcgag taatatcaat atcgattttc gaacaaaata
     5821 cattttttac ctcaaagtga tttaggactg ccaccttaca tattcaggtc agcatggcag
     5881 gggtcagata agcaccgtat tatattcaag aataatctct ggtttccgaa tttcggcacc
     5941 tgtctcataa atgattaacc ttagaaactg catcgaagcc tgatcctcca ggaattttag
     6001 ccgattcagt agtcttccca ggttaaatta tacagctatc agcctacgat taacatacga
     6061 aatgattcgg caggcaaaac agtccggcct tcaatcaaac cccgaaaatt accagaattg
     6121 cccattggag aagggtcaat caagaatata atccaccccg ttatgtttaa taaattattt
     6181 cggcaagcgg aaattcccgc tgaatatcgg tccaattttc ttcatcttta tctggatatc
     6241 gcatggtttg gtgtacttag cggctccgct ataaattttc tcagcatcta cgcagcccgt
     6301 atcggagcct caggttttca aatcggtatg attggtgcga tgtctgccct ggtcagtctc
     6361 ttcctcgcga ttccatctgg tcgctggctt cagacgcgga atacaggcag agccattttt
     6421 tgggcttccg ttctgtatcg aatatggtac ctgcctttta tttttttgcc gtggctattt
     6481 gatgggcagg gacaaatctg ggcgattatt gcgatcacgt ttctgatggc cattccactt
     6541 acgccgctgg gggtgggatt caatgcgctc tttgcagaag ctgttccaat tgagtatcgc
     6601 gcgcatgtcg ctggcattcg gaacattatg ttatccattg catttatggt cacatcgctt
     6661 gtcagtggaa ccattctgga gcgctcaacg ttcccggctg gctatcagat catttttgcg
     6721 attggagcgt ttggtgcggg gatgagcagt ttgcatttgt attttgtcag accccttctt
     6781 ataaatcgtt caacgaagga tgacggatcc ccaagagctg cgtcgacatc cccttccatt
     6841 aaggacaggc tttattcccg aatcatcgtt accgctcttc gcctggacat ctggaaaacc
     6901 tcgttcagaa aagttttatt atgtatgctg ggttttcacc tcacacaata tcttgcgatc
     6961 ccgatcttcc cgctctatta cgtaaatgaa ctgcatctta acgatgacca tattggcatc
     7021 ggcacggcat tattttatct aaccgtcctg atcggatcta cgcagttaag gaataccgtc
     7081 catcgagctg gcaacaagaa tgtgaccgcc tacggggtga tcggcatggc catctatcca
     7141 tttttacttg ccctgtctgc agaagtgtgg caattttatg gca
//
LOCUS       DASTJP010000088        10482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_984908, whole genome shotgun sequence.
ACCESSION   DASTJP010000088 DASTJP010000000
VERSION     DASTJP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984908"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..675
                     /locus_tag="VFQ23_02720"
     CDS             94..675
                     /locus_tag="VFQ23_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905518.1"
                     /translation="MKARENKPIIAMIILSVLLTMSCLLPGMIPLTPVPTVPAPVMET
                     NSETLIQTLQGQDWVYLQSLATEEYTSEELDRKTPGTLTFTINITDDKPTYFNYGWCT
                     TTQEVLEQNFEHITVKLYFNQEQLGNDVVHPVSFTRPDGLVCVDFGVMMSEWPNGTYT
                     LEAVSTFNEKINDGIADYEAGDYIFIYNVTVEK"
     gene            complement(754..1437)
                     /locus_tag="VFQ23_02725"
     CDS             complement(754..1437)
                     /locus_tag="VFQ23_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9905519.1"
                     /translation="MNLSVIIPVYNEAHNISEILKRVQATKLAKEIVIVDDGSKDGTR
                     DILSKLDGKKKVRVILHEQNQGKGAAVVTGLKAAKGDVLLIQDADLEYNPRDYPVLLQ
                     PIEEGVADVVYGSRFLGGPHRVTMFWHLVANKLLTFMTNILYNTILTDMETGYKVFRR
                     EVIEGMTIKAKRFNFEPEFTAKVLKSNHRIFEVPISFNPRDYSQGKKIKLRDAFEAVW
                     ALLKYRIMD"
     gene            1549..2880
                     /locus_tag="VFQ23_02730"
     CDS             1549..2880
                     /locus_tag="VFQ23_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905520.1"
                     /translation="MTNPLARPSPTGTTLFSPPPINSVDDTGLSALWLQDLTLKILYS
                     QGYMSGFKIAELIALPFAGVTDGILESLKREKMIEVKSSQGGLGEGAYTYGITGAGIT
                     RAREALERSQYAGPAPVPFEVYNEAIRRQKTGRMTVTSRTMRQLLSQLVLSESTFQRL
                     GPALNSGTSIFMYGPPGNGKTSVARAFGNLVLSQGMYIPYALYLDGQVIKVYDQVSHK
                     LAPDAERPSASSTGNLRTSQRRDPRWIKIRRPFIVVGGELTLEGLDLVYDEVHKFYEA
                     PFQVKANGGILLIDDFGRQQVRPRDLLNRWIVPLENRVDFLTLHNGRKVEVPFDVLIV
                     FSTNLPPKDLVDEAFLRRLRHKIEIGDPSYEEYREIFKRVAQDKRVEYSDQGLAYLLQ
                     EWYIKRNRKLRASHPRDLCDQILDIASYLAVPPTMSRDMLDRAALAYFVDI"
     gene            2953..3705
                     /locus_tag="VFQ23_02735"
     CDS             2953..3705
                     /locus_tag="VFQ23_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET9905521.1"
                     /translation="MLESLPQPVVFAHRGASAHAPENTLAAFELALAENADAIELDVK
                     LSADGHVVVIHDTTTDRTTGVQARVKNLSLAELRGLDSGSFFSEKFKNEKIPTLEEVF
                     EAVGKRTFINVELTNYSTPRDHLVESVCMLVKKFGLQKRVMFSSFFAMNLSKARGFLP
                     EVPRGLLAFNGWLGSWARSFGFAFGKYQALHPYLKDTTHPQVQRVHRLNRRVHVWTVN
                     AEDEMRRLFNWGVDGIFTDDPALAVKLREESR"
     gene            3702..6494
                     /locus_tag="VFQ23_02740"
     CDS             3702..6494
                     /locus_tag="VFQ23_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9905522.1"
                     /translation="MKNRLTSGRRIYPLHFLVWILLITISSSLIGPLPIARAQTPVPL
                     SKIQAVLNTMSPEERVGQLFLVTFRGTDLSDESQIRDLISNYHVGGVALLAENDNFIA
                     QPDTISGAHQLINALQGIEGEATSLSSENAYIPLFVGISQEGDGAPNDQILSGLTPMP
                     NPMAIGATWNTDLAEQMGTVLGSELSALGFNLFLGPSLDVVEAPNPSAQLDLGTRVFG
                     GDPFWVGEMGRSYIAGLHAGSSPRMMVVAKHFPGRGSSDRVPELEIATVRKPLEQLRL
                     VELAPFFAVTESTDPANLVDGLLVSHIRYQGFQGNIRATTRPVSFDPAALSEVIGQPE
                     FTTWRTNGGLVVSDALGSQAVRDFYSQGGETFSPRFVAQDAFLAGNDMLYLGNITSAE
                     AGSDTYSTTVGILEYFAQVYSSDATFARRVDEAVMRILAQKFRIYDVFTISNVLTTAQ
                     RLDTIGSSGQLMFEVARNSATLISPDLQELSTLLPAPPNQNERIAFLTDTSSYKQCGG
                     CLLHDGLAADALQRVVLRLYGPNGSAQVFTSRLSSFPFTELELMLNGESTTDIESFLE
                     NADWIVISLADTSEGQLTLLRRFFTERPNLLRNKNIILFSFTAPYYLDATDISKLHAY
                     YSLYSKQPAFVDVAARLLFQQASPEGHSPVSIPAIGYDLITETSPDPDQVISLALAEA
                     VSVTPTSEATPATAEPTQIPLYSIGDTIAVRAGPIVDHNQNIVPDDTVVRFTMSTREG
                     DASGSILQQVDAMTTMGIAYASFAINLPGKVEIRAVSEPAVISDVLQFDSSNEPAAVT
                     IVPVATLTPTVTVMPTVTVTPINDLITPEGYPRIGVWMLVLLAVFGGASLMFWAVSQI
                     VSPRWGLRWALCVLLGGLLAYNYLALGFPRAAEWIAAGAGAYGVLLLTFIGETVGALA
                     AWAWMKVVSEPTSQGG"
     gene            complement(6466..7464)
                     /locus_tag="VFQ23_02745"
     CDS             complement(6466..7464)
                     /locus_tag="VFQ23_02745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9905523.1"
                     /translation="MVLPPLKSGEVLRGRYKVRERIGQGGMGSIYLADDTRLKGRECA
                     LKEVEYDRALPENIRDEAREQFLREATVLARLDHPNLPKVSDFFSNGPRDYLVMDYIP
                     GNDLRTLMMDARRNKTFLKESEVLTWAEQIANALAFLHMQDPPIVHRDIKPSNLKLTP
                     HGLIKLVDFGLVKILAPEEVTITIIQGQGTALYTPLEQYGGSDVHTDIRSDIYSFGAT
                     LYHLLTNEAPADARKRFLHPDSLVPLRQINADITPRTERAVMWAMSLHPDERPESMDE
                     FRQSLLGSREIPILPVLGRRERPQSVLDYLSNTPEATLAWGATVLLLVSLLATLAR"
     gene            7799..8452
                     /locus_tag="VFQ23_02750"
     CDS             7799..8452
                     /locus_tag="VFQ23_02750"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9905524.1"
                     /translation="MMNKIQEDYPLLVAQEGPLKGQRWQLSQTLVLGRESTCDIVVSD
                     RQISRFHARISPTPEGVILEDLGSKNGTHHNGNPLAGPVVLQDGDLVSVALAQQFLFL
                     TSDATMPLVDGGKPGRLMMDQKSRRVWVNQQQLIPPLSAQQFKMLWLLYESKGQVVTR
                     PDLVATVWGDDQAAGVSDQALDALIRRLRDRLASLDPTHQYIATVRGHGIRLDNPSS"
     gene            8572..9147
                     /locus_tag="VFQ23_02755"
     CDS             8572..9147
                     /locus_tag="VFQ23_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905525.1"
                     /translation="MAGSFQNVVQIARREEIDMLDKVQITKLNALSGQERNKSTIWRY
                     IGVLGVMIFIMLLFAPAVITIAKFDLPALASLHVFMQKMARKDFESAYSQYAPQVKEK
                     MSSADLQEMVEESSSVMFEGYKGFTVRDFDLRVPYSTPDDSEPLDYSVKIEGTMFYQG
                     KSSRPFSAVLVLVDDRWAIESLQIDSRSSLP"
     gene            9161..9313
                     /locus_tag="VFQ23_02760"
     CDS             9161..9313
                     /locus_tag="VFQ23_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905526.1"
                     /translation="MFIKSLPTPRAADLGYAPRYLSFFLALGFSRFGGESTLPPQAAN
                     ANRWAA"
     gene            9345..10247
                     /locus_tag="VFQ23_02765"
     CDS             9345..10247
                     /locus_tag="VFQ23_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9905527.1"
                     /translation="MPSWQFLLIKTIFQIRRLLNPPTGVLDVEEVRAEDEALAANFRT
                     KIELTCIPVIANGVPAEWITTPTSSADTVILYLHGGSYISGSITSHRSLAANIIHAAK
                     ARALLVGYRLAPEYRFPSAVDDARAVYRWLLENHVTPDHIIVAGDSCGGGLVLSLLIS
                     LRDEELPLPAAAVCLSPWTDLTCTGESWETNRKKDIMLDPGALRKSAQLYLGEANPRT
                     PLASPLYGDLKALPPILIQVGSNELLLSDATGFAKRAQAAGTKVTLQVWNGMQHEWHF
                     AVNILPEARQAIQQIGQFIQAQIG"
BASE COUNT         2498 a         2661 c         2673 g         2650 t
ORIGIN      
        1 tgaagagtat gagtgcaatg cgcaatcttt tattatacca atccgtttcc ctaagaatat
       61 gtttataatc tgtcaccacg aaggaggtgg cttatgaaag ccagagaaaa taaacccata
      121 atcgcgatga tcattctgag cgtgctgctc acgatgtcat gtttactgcc aggtatgatt
      181 cctctcactc ctgtccccac tgtccctgcg ccggtcatgg agacgaattc tgagacgctg
      241 attcagaccc tgcaggggca ggactgggtc tatctgcaat cgcttgccac ggaggaatat
      301 acctccgagg aactggacag aaaaacaccc ggcacactta cgtttacaat aaatatcact
      361 gatgataagc caacctattt taattatggc tggtgcacga ccacgcagga agtccttgag
      421 cagaattttg aacacataac cgttaaactt tattttaatc aagagcagct tgggaacgat
      481 gtggtgcatc ctgtttcatt tacccggccc gatgggcttg tgtgcgtgga ttttggtgtg
      541 atgatgtcag agtggcccaa cggcacgtat acgctggagg ctgtttccac tttcaacgaa
      601 aagatcaacg acggaattgc cgattacgaa gcaggagatt atatttttat atacaatgtg
      661 accgttgaga aataaaaaaa aggcgtgata cgcctcttca cattttcgta ttcctcgctc
      721 tcacaagttt cagaccttgt gagagcgaac tcattaatcc ataattctgt atttcaacaa
      781 ggcccacact gcttcgaacg catccctcaa tttgatcttc ttcccctgcg agtaatcacg
      841 tgggttgaaa gagatgggca cttcaaaaat gcgatggttg cttttcaaca ctttcgccgt
      901 aaactccggt tcaaaattga atcgtttggc tttgatggtc atcccctcga tcacttcgcg
      961 gcggaaaact ttgtaccccg tttccatatc ggtcaggatg gtattgtaca gaatattggt
     1021 cataaacgtc agcagtttat tggcgaccag atgccagaac atggtcacgc ggtgcggccc
     1081 gccgagaaaa cgtgaaccat agacgacatc tgccacccct tcttcaatcg gctgcagaag
     1141 cacgggataa tcacgcgggt tatattccaa atcggcatcc tgaatcaaca aaacatcccc
     1201 tttggcggcc ttcaagcctg taacaacggc tgctcccttg ccctgattct gctcatggag
     1261 gatgacgcgt accttttttt taccatctaa cttgctcagg atgtctcgcg tcccgtcttt
     1321 tgagccatca tccacgatca caatttcttt tgccagcttt gtcgcctgta cccgcttaag
     1381 gatctcactt atgttgtgtg cttcattgta gacaggaatg atcacagaaa ggttcatggt
     1441 gcgccgatta tatacgaaat taatggcggt ggagattaga tacaagaagc agtataattc
     1501 ggatagatca aaatctgaac cacggagcat cccagaccgg gagatgttat gaccaatccc
     1561 cttgctagac catcaccaac cggtacaact ttattttctc caccaccgat caactcagtg
     1621 gatgatacgg gtctttcagc cctgtggctg caggacctca cgttaaaaat cctgtattca
     1681 cagggataca tgagtggttt taagatcgcg gaattaatcg cattgccttt tgcaggtgta
     1741 actgatggga ttctggaatc gctcaagcgt gagaaaatga tcgaggtgaa atcttcacag
     1801 ggcgggttgg gtgaaggagc ttatacgtac ggcatcactg gcgcaggcat cacccgggca
     1861 cgtgaagctc tggagcgcag tcaatatgct gggcccgcac cggtaccgtt tgaagtctat
     1921 aacgaagcga tccggagaca gaagaccggt cgcatgaccg tcacgtctcg gacgatgcgc
     1981 cagctccttt cacaattggt tctctctgaa tccaccttcc agcgactagg ccccgcgctt
     2041 aattcaggta catccatctt tatgtatggc cctccgggaa atgggaaaac cagtgttgcg
     2101 cgcgcatttg gtaatttagt attgagccag ggaatgtata ttccctacgc actgtatctg
     2161 gatggacagg tgatcaaagt ctacgaccag gtgagccata agctggcacc cgatgccgaa
     2221 agaccatctg ccagcagcac cggaaatctg cgcacgagcc agcgccgcga cccacgttgg
     2281 atcaaaattc gccgtccatt tattgtggtg ggcggtgagt tgaccctcga agggctggat
     2341 ctcgtatatg acgaagtgca taagttttac gaggcgccgt ttcaggtcaa agcgaacggt
     2401 gggattctgc tcatcgatga ctttggaagg caacaggtcc ggccgcgtga tctgctcaac
     2461 cgctggatcg tcccacttga aaaccgtgtg gacttcctta ctcttcacaa cggacgcaaa
     2521 gttgaagtcc cgtttgatgt cttgatcgtc ttctccacca atctgcctcc caaggacctc
     2581 gttgatgaag cctttttgcg tcgcctcagg cacaagatcg agatcggcga tccgtcctat
     2641 gaagaatacc gcgagatctt caaacgggtt gcacaggata aacgggtgga atacagcgat
     2701 cagggcttag cctatctctt gcaggaatgg tacattaaac gcaatcggaa attacgcgcc
     2761 tcgcatccgc gtgacctttg tgatcagatc ctggatattg ccagttatct ggcggtccca
     2821 ccgaccatgt ctcgtgatat gcttgatcgt gccgcgcttg cctattttgt tgatatttag
     2881 gcttactttt caatcacacc ctacggatta cgcatcatgc acgtgtggtg tgtaatccgt
     2941 aaattattta tcatgctgga atcactcccc caacctgtcg tctttgctca caggggcgcc
     3001 tcggctcatg ctcctgagaa tacgcttgcc gcgtttgaat tggcacttgc tgaaaatgcg
     3061 gatgcgattg aactcgatgt caagttgagc gccgatgggc atgtggtggt gatccacgat
     3121 acgacaactg accgcacaac gggcgtgcag gcgcgtgtca aaaacttatc tctcgcagaa
     3181 ttgcgtgggc tcgactccgg tagtttcttt tcagaaaaat tcaagaacga aaagatacca
     3241 acgcttgaag aagtctttga agctgtgggg aaacgcacat tcatcaacgt cgagctgacc
     3301 aattacagca caccgcgcga tcatctggtt gaatcagttt gtatgctggt aaaaaagttc
     3361 ggcctgcaga aacgcgtcat gttttcatcc ttcttcgcta tgaatctttc caaagcgcgc
     3421 ggtttcctcc cggaggtccc gcgtggattg ctggccttca acgggtggct gggttcctgg
     3481 gcgcgttcct ttggttttgc atttggtaaa tatcaggcat tacatcccta cctgaaggat
     3541 acaacacatc cacaggtaca gcgcgtgcat cgcctcaacc gacgcgtcca cgtgtggacg
     3601 gtcaacgcgg aagacgaaat gcgtcgtttg ttcaattggg gtgtggacgg aattttcaca
     3661 gatgacccgg cactggcagt aaagctgcgc gaggaatcca gatgaagaat cggttaacgt
     3721 cgggcagaag aatctacccg ctacatttcc tcgtctggat tcttctgatc acgatcagtt
     3781 cctcattaat cggacctttg cccatcgcgc gtgcccaaac ccccgtgcct ttatcgaaaa
     3841 ttcaggctgt gctgaatacg atgtctcctg aggagcgagt gggacaatta tttctggtca
     3901 cctttcgcgg cacagaccta tccgatgaat cccaaattcg tgatctcatc agcaattatc
     3961 acgtaggcgg agtggcctta ctagccgaga acgacaactt catcgctcaa ccagatacga
     4021 tctccggcgc gcatcaactg atcaatgcat tacagggcat agaaggggaa gcaacctcgt
     4081 tgtcttcaga aaacgcctac attcctttgt ttgtggggat ttcacaggaa ggtgacggcg
     4141 cacctaacga tcagattcta agcgggttga ctcccatgcc caacccgatg gcgattggtg
     4201 caacctggaa caccgatctt gcagaacaaa tgggtacggt gctgggtagc gaactcagcg
     4261 cattgggatt caatttattc cttggtccat cactggatgt cgttgaggct cccaatccgt
     4321 ccgcgcaact tgacctgggt acgcgtgtct ttggtgggga cccgttttgg gtgggtgaaa
     4381 tgggacgctc ctacatcgcg ggtctgcacg ctggcagtag tccgcgcatg atggtggttg
     4441 ccaaacactt tcccgggcgt ggcagttcag accgtgtgcc tgaactggaa attgctacgg
     4501 ttcgcaaacc gctggagcaa ttacggctgg tcgaactggc accgttcttt gctgtgaccg
     4561 aatcaactga tccggcaaat ttagtggatg ggctgcttgt ttcacatatt cgttatcagg
     4621 gctttcaggg aaatatcaga gcgaccacgc gtcctgtcag ttttgaccct gccgcgcttt
     4681 cggaagtgat cggacagcct gaatttacaa cctggcgtac aaatggcggc ttagttgtta
     4741 gtgacgcgtt gggaagccag gccgttcgcg atttctattc tcagggggga gaaacgttct
     4801 cgccacgctt tgtggcgcag gatgcattcc tggctgggaa tgatatgctc tatcttggca
     4861 atatcacctc cgcggaagct ggcagcgata cctattccac aacagtcgga atccttgagt
     4921 actttgcaca ggtctatagt agtgatgcca cttttgcaag acgcgtggat gaggctgtca
     4981 tgcgaattct tgcccaaaaa ttccgcatct atgatgtttt tacgatttcc aacgtgctca
     5041 caaccgccca gcgattggat accataggtt cctcaggaca actcatgttt gaggtggcac
     5101 gcaacagtgc cacactgatc agcccggatc ttcaggagtt aagcacactc ctgcctgctc
     5161 ccccgaatca aaatgaacgt atcgccttcc tcaccgacac atccagttat aaacagtgcg
     5221 gcggatgtct gctgcatgac gggctggctg ccgatgcatt gcaacgcgtg gtcctgcggc
     5281 tatacggacc taatggcagt gcacaggtgt ttaccagtcg cttgagttcg tttccattta
     5341 cggaacttga gttgatgttg aacggcgaaa gcacaacaga tatcgaatca tttttagaga
     5401 acgccgactg gatcgtgatc tctctggccg atacgagtga gggacagctt acattattac
     5461 gccgcttttt tacagaacgt cccaacctgc ttcggaacaa gaatatcatt ctcttttcgt
     5521 tcacagcccc ctattatctt gatgcaacag atatctccaa actgcacgcg tactattcgc
     5581 tttatagcaa acaaccagca tttgtggacg tggcagcccg attgttgttt cagcaggcat
     5641 ccccggaagg ccattcgccg gtctccattc ccgccatcgg atacgacctg attacagaga
     5701 cctcaccgga ccccgaccag gtgatttccc tggcgctggc tgaggcggtc tccgtaactc
     5761 cgaccagtga agcgacgcct gccacggcag agccaacaca gattccactg tacagcatcg
     5821 gcgatacgat cgcggtgcgt gctggtccga tagtagatca caaccaaaat attgtcccgg
     5881 acgataccgt ggtgcgattt accatgtcca cgcgggaagg cgatgccagt ggttcgatcc
     5941 tgcagcaggt tgatgcaatg acaactatgg gcattgcgta tgcttccttc gcgatcaatt
     6001 tgccagggaa agtggaaatc cgtgcagtca gtgagccggc agtgatctcc gatgtgctgc
     6061 aattcgattc atccaacgag cccgctgcag tgaccatcgt gcccgtagca accctcaccc
     6121 ccacagtgac cgtaatgcca acggtgacgg tgactccgat taatgacctg atcacaccag
     6181 aaggatatcc gcgcattggg gtgtggatgc ttgtcttgct ggcagtcttt ggtggtgcat
     6241 cgttaatgtt ctgggcagta agccagatcg tttccccgcg ttggggctta cgctgggcat
     6301 tgtgcgtttt gctcggcggg ttgcttgcct ataactatct cgcgttggga ttcccccgcg
     6361 ccgcggaatg gatcgcggcc ggggccgggg cctatggtgt attactctta acgtttattg
     6421 gggaaacagt cggcgcgtta gcggcctggg cctggatgaa agtggttagc gagccaacgt
     6481 cgcaaggagg ctgaccagca gcaagacggt cgcgccccag gccagagtcg cctcgggcgt
     6541 gttggataga taatccagca cagattgtgg gcgttcgcgg cgcccgagca cagggaggat
     6601 tggaatctca cgagatccca gcagcgattg acgaaattca tccatggatt ccggccgctc
     6661 atccgggtgg agcgacatgg cccacatcac ggctcgttcg gtgcgcggtg taatgtccgc
     6721 gttgatctgc cggagcggga ccaggctatc ggggtgtagg aagcgtttgc gcgcatctgc
     6781 cggagcttca ttggtaagca ggtggtagag cgtggcaccg aacgaataga tatccgagcg
     6841 gatgtctgtg tgaacatcgc ttccaccata ttgttcgagt ggggtataca gtgcggtgcc
     6901 ttgcccctga atgattgtga tcgtcacttc ctcgggcgca aggatcttca ccaggccgaa
     6961 gtccaccagc ttgatcaggc cgtgcggcgt caactttaga ttgctgggtt tgatatcgcg
     7021 gtgaacgatg ggagggtcct gcatgtgtaa gaaggccagc gcattcgcaa tttgctctgc
     7081 ccaggtgagc acttcgctct cttttaagaa cgtcttgttt cggcgggcat ccatcatgag
     7141 cgtgcgcaag tcattccccg gaatgtaatc catcacgaga taatcgcgtg gaccattgga
     7201 aaagaaatca gatactttcg gcaggttcgg gtgatcgaga cgcgcaagca cagtagcctc
     7261 gcgcaggaat tgctcgcggg cttcgtcacg gatgttttcc gggagggcgc ggtcgtattc
     7321 gacttccttc agcgcacatt ctcgaccctt gagacgggta tcatcggcaa gatagatcga
     7381 acccatgccg ccctgaccga tccgctcacg gaccttgtag cgaccacgca atacctcccc
     7441 gcttttgagg ggtggaagca ccattctcct ttataactta catgctcggc gtacgctttg
     7501 tttgttcatt atcggtcatc catcgaaaat ggaacaaacc cgatctgcgc agagttttcg
     7561 gcgttggttc tgcacagaaa cgcgtgttta tgctatatta caaagcgcca cggtacctct
     7621 ccaacagttt ttgcttacag tttcatcaaa aagttttgta ccgctggcaa tgaatagtat
     7681 tattatactg cctgttgagt tatactcaaa aagaatttgt gatctgcata ttcaccatct
     7741 ctgatgattc cttgcgtcac tgctcacacg gctcgaaaga atcatttttg agaccttcat
     7801 gatgaataag atacaggaag attatcctct acttgtggct caggagggac cgctcaaggg
     7861 ccaacgctgg cagctcagcc aaacgcttgt attagggcgc gagtccacct gtgatattgt
     7921 cgtttcagac cggcaaattt cacgcttcca cgcgcgcatc tcgccaactc cagaaggtgt
     7981 catcctggaa gatttgggga gcaagaacgg cacgcaccat aacgggaatc cgctggcggg
     8041 cccggtcgta ttgcaggacg gtgaccttgt ctcagtggcg ctggcacagc aatttctctt
     8101 cctaacatca gatgcaacca tgccgctcgt ggacgggggc aagcctggtc ggttgatgat
     8161 ggaccagaaa tcgcgccggg tgtgggtcaa tcagcagcaa ttgatcccgc ctctttcggc
     8221 acagcaattc aagatgttat ggctgctcta tgaaagcaag ggacaggttg tcacacgacc
     8281 tgatctggtc gctaccgtgt ggggagatga ccaggccgcc ggcgtatctg accaggcatt
     8341 agatgccctg atccgccgcc tgcgcgaccg cctcgcctcg cttgacccca ctcaccaata
     8401 catcgccacc gtgcgcgggc acggcataag gctggataac ccgtcttcgt aaattagagt
     8461 tttttcggta tcagtgcttt tcttttaatc tatttccgct taatccccca aattgggata
     8521 ttagacggcg tccgtctata agctcttatg cggagtccgt cgaatctcta gttggcaggc
     8581 tccttccaga acgtggttca gatagcaaga agagaagaaa tcgatatgct tgataaagta
     8641 caaatcacta agctaaatgc tttaagtggg caagaaagaa ataagtcaac catctggcgg
     8701 tatattggcg ttctcggggt aatgattttt ataatgcttc tgttcgcacc agcggtgatc
     8761 accattgcta aattcgatct tccagcctta gcaagcttgc atgtattcat gcaaaagatg
     8821 gctcgcaagg attttgaaag tgcttactcc caatatgccc cgcaagttaa agaaaagatg
     8881 tcctcagctg atttacagga aatggtggag gaaagtagct ctgtcatgtt tgaaggttat
     8941 aaaggattta ctgttcgaga ttttgacctt cgtgtacctt atagtacacc tgatgattcg
     9001 gaaccacttg actatagtgt taagattgaa ggcacgatgt tttatcaagg caaaagtagt
     9061 cgtccattta gcgcagttct tgtcctggtc gatgatcgtt gggcaattga atctttacag
     9121 atcgattcac gatcaagcct gccgtaagga aaagttatgc gtgttcatca aaagcctgcc
     9181 aacaccgcgt gcagccgact tggggtacgc gccgcgttat ttgagttttt ttctggcgtt
     9241 aggcttttcc cgtttcggcg gcgagtccac gctcccaccc caagcggcta acgccaaccg
     9301 ttgggcagcc taaagaattt ggttgtgaaa ggagattgaa cataatgcca agctggcaat
     9361 tcctcctgat caaaacaatt tttcagatac gacgtctcct taatccgcca acaggagtgt
     9421 tggatgttga agaggtacgt gccgaggacg aagcactggc agcgaatttc aggaccaaga
     9481 tcgagctaac ctgtatacct gtaatcgcca acggagttcc ggcagagtgg atcactacgc
     9541 ctacgagctc agcagataca gtgattctat atctacacgg tgggagctac atcagtggtt
     9601 cgatcacatc tcatcgttca ctcgccgcaa atatcattca tgccgcgaaa gcccgcgctt
     9661 tgcttgtcgg ttaccgatta gctccggaat accgttttcc atccgcggtt gacgatgcca
     9721 gggcggtcta ccgatggctg ctggaaaatc atgtcacccc agatcatatt attgttgccg
     9781 gcgattcgtg tggtggtggg ttagtactct cacttcttat cagcctacgc gatgaggagt
     9841 tgccattacc agccgcagcg gtttgtctgt ctccttggac agatttaact tgcacgggag
     9901 aatcttggga aacaaatcga aaaaaagata tcatgctcga tcccggcgca ttgagaaaat
     9961 cggctcagct atatctgggg gaagcaaatc ctcgcacgcc acttgcatct ccgctttatg
    10021 gagacttgaa ggcattgcct ccgatactca ttcaagtagg ctctaacgaa ctccttcttt
    10081 cagatgctac aggttttgca aaacgggctc aagcggctgg caccaaggta acacttcagg
    10141 tgtggaacgg tatgcaacat gaatggcatt ttgctgtgaa tattcttcca gaagctagac
    10201 aggctattca gcagattggt caatttattc aggctcagat cggctgagta tagagcaaag
    10261 gttgcccaac acagcgtgca cccgactggt gggagtctgc gcgttttcag gcagtttgca
    10321 tggcttgagg ttggttccgt caaagtagcg ttatctcgtc ccgcccacca gcgggtaacg
    10381 caagccgtta gccgcataga agagaaagtc tagtgctatg tcaatgaata caggaagtta
    10441 tctaaagaga acaaatcgaa cacacttatc cgaagcagaa tt
//
LOCUS       DASTJP010000089         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_332282, whole genome shotgun sequence.
ACCESSION   DASTJP010000089 DASTJP010000000
VERSION     DASTJP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332282"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1391)
                     /locus_tag="VFQ23_02770"
     CDS             complement(183..1391)
                     /locus_tag="VFQ23_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905528.1"
                     /translation="MDISLLFTTRIIRLFCYGFLSVVLALYLSEIGLTEPQIGLLFTL
                     TLIGDAVITLWLTTSADRFGRKRILLVGAFLMAAAGIVFVMTRNYLLLILAAVIGVIS
                     PADKDIGPFLSVEQASLTHLIANENRTQVFAWYNLAGSFASAAGAFTGGWLAQGLQND
                     GWAAFESYRAVLIGYAFGGLILFIVFFNLSHSIEIEKPPQDDIRRILGLHRSRNVVFK
                     LSSLFALDSFAGGLLVQSFLAYWFYVKFGVDTGILGSIFFGGNILAGISALLAVRISK
                     KFGLINTMVFTHIPSSILLMMIPWMPTLWPAVVLLLLRFSISQMDVPTRQSYTMAMVD
                     PDERSAASGVTAIARSLGSAVSPALTGLFFSVPALFNLPFFLCGGLKIIYDLLLFREF
                     RTVKPPEENI"
     gene            complement(1576..2010)
                     /locus_tag="VFQ23_02775"
     CDS             complement(1576..2010)
                     /locus_tag="VFQ23_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HET9905529.1"
                     /translation="MKWITRSHVHVDRVACPWLIRRFVDNEAEFLFVPKSQIDKVARE
                     TGAIPFDAPGVELGHHEGRCSFESVLLKYDLKDAGLLRLAKIVHAADVAEDIDTDPLA
                     RGLEAIASGYSLRFPDDMENIEHQFEVYDALYAWCRMDVVKD"
     gene            complement(2056..2451)
                     /locus_tag="VFQ23_02780"
     CDS             complement(2056..2451)
                     /locus_tag="VFQ23_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905530.1"
                     /translation="MTTDPAILFVCEHGAAKSVIAAEYFNKLAAEAGLGSRAVARGTD
                     PDDELSPKAIQGLREDGLAPNESAPQKLSLADVESAQQIITFCELPKEYEPKAIVEHW
                     DHVPPVSEGYENARDAIVEQIDSLIHRLR"
BASE COUNT          679 a          668 c          662 g          605 t
ORIGIN      
        1 ggtgttccac catattcaag cagactcagg caagcatttc gatccgcggg ttgtgaatgc
       61 gtttatggaa atgagcgatt tgtcagtttg atattccagc ctgtaagggg cggcagctcg
      121 cgacacttgc gcctgtgcgc agtgcaggtg agcaccctgt cggggtgccg tccactacag
      181 gcttatatat tttcctccgg gggttttacg gtacgaaatt cacgaaacaa cagcaggtca
      241 taaatgattt tcaatccacc acagagaaaa aaggggaggt tgaacaacgc gggtacgctg
      301 aagaacagac cggtcaacgc gggtgaaacg gccgagccga gggagcgcgc gatggcggtc
      361 acgccggaag cagcggaacg ttcgtcgggg tccaccattg ccatcgtata ggattgccgt
      421 gtgggaacat ccatttgcga gatgctaaag cgtaacagga gtaagacaac tgcaggccac
      481 agggtaggca tccaggggat catcatcagc aaaatgctgg aagggatatg cgtaaagacc
      541 atggtattga tcaaaccgaa ttttttggag atgcgcacag ccagtaaggc tgagattccg
      601 gcgagtatgt ttccgccaaa aaaaatgctg ccgagaatcc cagtgtccac accaaatttg
      661 acgtaaaacc agtaggcaag gaagctttgt acgagtaacc cacccgcaaa cgaatccagt
      721 gcgaataacg aacttaattt gaatactaca tttcgggagc gatgcaaacc tagtattcgc
      781 cggatatcat cctgtggtgg tttttcaatt tcaattgaat gacttaaatt gaagaaaaca
      841 ataaagagaa tcagtcctcc aaacgcgtat ccgatcagga cagcacggta cgattcgaat
      901 gcggcccaac cgtcgttttg taacccctgc gcaagccagc cgcctgtgaa cgcacctgcg
      961 gcggatgcaa atgaacctgc caggttgtac caggcgaata cttgagtgcg attttcgttg
     1021 gcgatgagat gcgtcaggct ggcctgctcg accgaaagga acggtccgat atccttatcc
     1081 gccgggctga tcacaccgat gacggctgcc aggatcagca ggagatagtt tcgtgtcatt
     1141 acaaacacga tgcctgcggc ggccatcagg aacgcgccaa caagcaagat gcgttttcgg
     1201 ccaaaccggt ctgcggacgt ggtgagccac agggtaatga ccgcgtcgcc gatcagggtt
     1261 aatgtgaaca gtaaaccaat ctggggttcg gtcagtccaa tctcagagag atacagcgct
     1321 aagacaactg acaaaaatcc gtagcagaaa agacgaatga tacgcgtggt gaagaggagg
     1381 gagatatcca tttagtttcc ggtggtcgag tagggtcaag cgatagcaag acccgtatcg
     1441 agaccacctc tcagtaaagc aattcttgtt aagcaccggt cactgcacgt gaattgcttg
     1501 gcaaatcatg caagtgtctc aagtaacctc caaacaggag attgcttcga gctatcgccc
     1561 tcgcaatgac atcagttaat cttttacaac gtccatccgg caccacgcat acaacgcgtc
     1621 gtagacctca aattgatgtt cgatgttctc catatcgtcc ggaaagcgca gactgtatcc
     1681 tgatgcgata gcttccagtc cgcgtgcaag cgggtcagta tcgatatcct ctgcaacgtc
     1741 cgccgcatgg acgatctttg ccagccgcaa taaaccggca tccttgagat cgtatttcag
     1801 gaggaccgac tcaaaggagc agcgtccctc atgatggcct agttccaccc cgggcgcgtc
     1861 gaatggaatc gcacccgttt ccctggcgac cttatcaatt tggctctttg gcacaaacag
     1921 gaactctgct tcgttatcca cgaaacgcct aatgagccag gggcaggcga ctcgatccac
     1981 atggacatga gaacgtgtga tccatttcat taggtactcc ttttagccag gtgacagtca
     2041 tttctaaaat gactgtcacc ttaacctgtg tatcaacgaa tcaatctgct caacgatcgc
     2101 atcccgagcg ttttcataac cttcgcttac aggcgggacg tgatcccagt gttcgacaat
     2161 cgcctttggc tcgtattctt ttggcaattc acaaaaagtg atgatctgct gagcagactc
     2221 tacatccgcc agagataatt tttgaggagc cgattcattt ggagctaagc cgtcctctcg
     2281 caagccttgg attgcctttg gcgataattc gtcatccgga tctgtcccgc gcgcgacagc
     2341 tcgggaaccc agcccggctt cagccgccaa cttgttgaaa tattccgcgg cgatcacgct
     2401 ttttgctgcc ccatgttcac acacaaataa gatagcagga tcggttgtca taaattcctt
     2461 tttcggttct atttatcaga gtcaattttt agttgcgcat acaacgcatc ataaatcata
     2521 tatccgcgtt cgagcgcttc gtaatcatct ttgctgattt gtctcagccc gcggcagatg
     2581 aggtcaagcc ctgctgcggc tggctccagc gtga
//
LOCUS       DASTJP010000090         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_569814, whole genome shotgun sequence.
ACCESSION   DASTJP010000090 DASTJP010000000
VERSION     DASTJP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569814"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VFQ23_02785"
     CDS             complement(<1..945)
                     /locus_tag="VFQ23_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9905531.1"
                     /translation="MKKTAFFLLLIFLSSACSPGSAEPQTLTIMTHDSFAISEAVVTA
                     FEEANNAQVEFLASGDAGAVLNKAVLTKDAPLADMLFGVDNTFLSRALEADIFETYDS
                     PVLQEIPDEFKLDGSNRAVPVDYGDVCINYDKAYFAENNLAIPQSLEDLTKPEYNGLL
                     VVENPATSSTGLAFLLATIAHYGDGFQDYWRALKDNGVVVVDGWETAYYTNFSGSSGN
                     GPQPLVVSYGSSPAAEVVFSETPLNDAPTASIIGPDTCFRQIEFVGILKGTEQRALAE
                     KFIDFMLDVQFQEDMPLQMFVYPVNPNANLPEQFVKYAQ"
     gene            complement(971..1612)
                     /locus_tag="VFQ23_02790"
     CDS             complement(971..1612)
                     /locus_tag="VFQ23_02790"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET9905532.1"
                     /translation="MHRIIIFANGDLPHLEKARLLLQPDDHIICADGGARHALALGLT
                     PHLVIGDMDSINKDDWQHLEKSGVSIDLFPQDKNETDLELAIHRAVELSPEELLIVGA
                     LGGRLDQMLGNIALLSDNRLAALHARLDDGVEEIFFCRDQVQVHGRPGDIVSLIPWGN
                     AVYGVQTRDLKWPLKSETLYPNQSRGISNEVTANTSSIKIDAGLLLIVHRRQS"
     regulatory      complement(1685..1796)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1832..2656)
                     /locus_tag="VFQ23_02795"
     CDS             complement(1832..2656)
                     /locus_tag="VFQ23_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9905533.1"
                     /translation="MSAILLDGSIVHYEVLGRGRPVIFLHGWVGSWRYWISSMQVAST
                     SYRAYALDLWGFGDSAHNESKYSLDDQASLIDRFLIEMGIGKIALVGHGLGGLVGMAF
                     ANRFPQNVDRMMAVNTPLHIEGVSSRLRATSPSPSELTDWLSSRTPEATTALSDSSKA
                     DGKAIATSMDSLLNNNPYNKFRNVERRDLNSTPDPSTIPCLLVYGEKDQAIFAPEEGF
                     PFSSMTHQVLLDGSGHFPMIDETIKFNRLLTDFLALEAGTSPSELQMKEEWRRRVR"
     gene            complement(2660..3034)
                     /locus_tag="VFQ23_02800"
     CDS             complement(2660..3034)
                     /locus_tag="VFQ23_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9905534.1"
                     /translation="MAKILIAEDERDIRDLVAFTLRFAGYEVFAATNGEEAVEMAPKV
                     NPDLILMDVRMPRMTGYEACKVMKANPDLKDIPVVFLTARGQETEIQQGLDAGAEEYL
                     LKPFAPDQLTTRVKAILAKFGK"
     gene            complement(3042..>4019)
                     /locus_tag="VFQ23_02805"
     CDS             complement(3042..>4019)
                     /locus_tag="VFQ23_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905535.1"
                     /translation="LSLESGRVIGQSLDVFGGLFGKSAGPWMQTIQNWSDSPAAYQQG
                     DTFAEQIDLENGQIILVHLAPVILQSDFLGTVSIFRDITHEVEVDRLKSEFVATVSHE
                     LRTPMTSIRGYVDVLLMGAAGAMNENQTHFLNIVKNNTERLNILVNDLLDISRIESGR
                     VTLSPQALDLREIAEDVIGDVLRRSQEENKPMALSLDAPKKLPAVYGDVERVRQIMGN
                     LVDNAYHYTPENGTITVHIHPLNGGHEIQVDVQDNGVGISLEDQDRVFERFYRGEHPL
                     VLATPGTGLGLSIVKQIVEMHQGRIWMKSNGVPGNGSTFSFTLPVYEKQ"
BASE COUNT         1016 a         1048 c          969 g          986 t
ORIGIN      
        1 ctgagcatat tttacaaact gctcaggcaa atttgcattt gggtttaccg ggtagacaaa
       61 catttgcaaa ggcatgtctt cttgaaattg tacgtctagc ataaagtcaa tgaacttttc
      121 ggctagggca cgctgctcag tccctttgag gatgccaacg aactcgatct gtcggaagca
      181 cgtatcgggt ccgatgatcg aggcggtcgg cgcgtcattc aacggcgtct ctgaaaagac
      241 tacttccgcg gcgggagaac ttccgtagga aacaaccaga ggctgaggtc cgttcccgga
      301 agagccgctg aaattggtat agtacgcggt ttcccagcca tccacgacga ctacgccatt
      361 atctttcaat gcgcgccagt aatcctgaaa cccatctcca tagtgcgcaa tggtggccaa
      421 tagaaaggca agtccggtag atgaagtagc agggttttcg accacgagca aaccgttgta
      481 ttcaggcttc gtcagatctt ccaggctttg aggtatagcc agattattct ccgcaaaata
      541 ggctttgtca tagttgatgc aaacatcacc gtagtcaacc ggcacggcgc ggttcgatcc
      601 atcgagtttg aactcatcag gaatttcctg caagacgggt gagtcgtagg tttcaaatat
      661 atcagcctca agggcacgcg agagaaaggt attatccact ccaaaaagca tgtcggcgag
      721 cggcgcgtct ttggtgagga cggccttgtt caggacagcc cctgcgtcac cgcttgccag
      781 aaactcgacc tgtgcattat tggcttcttc aaaggccgtt actacagcct cactgatcgc
      841 aaatgagtcg tgagtcatga ttgtaagtgt ttgaggttca gcagagcctg gcgaacaggc
      901 tgaggataag aaaattaaaa gtaggaagaa agctgttttt ttcatagagc ctttcgagta
      961 tgacctaaat ttatgattga cgacgatgga caatgagcag caatccggca tcgatcttaa
     1021 tgctcgatgt attcgctgtc acttcattgc tgatgccgcg cgattgattc ggataaagcg
     1081 tttcactctt caggggccat ttgagatccc gagtttggac accataaacc gcattacccc
     1141 acggaatcaa tgagacgata tctcctggtc ttccgtggac ttgtacctga tctcggcaaa
     1201 agaatatttc ctccacccca tcgtcgagtc gggcatgcag cgccgcgagc cgattgtcgc
     1261 taagtaaagc aatattgccc agcatttgat cgagtcgtcc acccagcgcg ccgacaatga
     1321 gaagctcttc agggcttaat tcaactgccc gatggatggc aagctcaagg tcagtctcat
     1381 ttttgtcttg tggaaataag tcaattgaaa cgccagactt ttccaaatgc tgccaatcgt
     1441 ctttgttaat tgagtccata tcaccgatca caagatgggg cgtcaagccc aaggcaagcg
     1501 cgtgacgagc cccaccatct gcacagataa tgtgatcgtc aggttgcagt aacaggcgag
     1561 ccttttcaag gtgaggcagg tcgccgttgg caaagataat aatgcggtgc atgaagccct
     1621 caaccctacc ctttcccaat gggagaggga gttaatgaaa aaccatcctc gcggcggagg
     1681 atggttgata gttaaaacaa tggtccctcc gccagcatga tctggatcag gttatgcggg
     1741 tcgagcgcgg ctcgctcctc tcagtcctgc atcgcgggac accccgttca attgtgcaac
     1801 tatcgtaata cgtttctgag tgaatgtcaa cctagcgaac gcggcgccgc cactcttctt
     1861 tcatttgcaa ttcgctgggg cttgtgcctg cttccagtgc aaggaaatcc gtcaataatc
     1921 gattgaactt aatcgtctcg tcaatcattg ggaaatgtcc cgaaccatcc aataagacct
     1981 ggtgagtcat tgatgaaaaa ggaaagcctt cctcgggtgc gaagatcgcc tgatcctttt
     2041 ctccataaac aagcaaacac ggtattgtgg atggatccgg cgtggaattg agatcacgtc
     2101 tctccacatt gcgaaattta ttataaggat tattgttcag caggctatcc attgaagtgg
     2161 caatggcctt tccatctgcc ttcgaagaat cagacaatgc ggtggtcgct tcaggcgtgc
     2221 ggctggataa ccagtctgtc agttcagatg gagaaggcga agttgctcgc aggcgtgagc
     2281 ttactccctc aatatgtaac ggagtattga ctgccatcat acgatctacg ttttgcggaa
     2341 aacgatttgc aaatgccatg ccaaccaagc cacccaaccc gtggccaaca agagcaatct
     2401 taccaatacc catttcgatc agaaaacggt caatcaggga tgcctggtca tcgagggaat
     2461 actttgattc attgtgcgcg ctatccccga agccccataa atccaacgca taggcgcgat
     2521 aggatgtcga ggcgacctgc atggaagaga tccaatatct ccatgagccc acccagccgt
     2581 gcagaaagat gacaggacga ccgcgtccta gcacctcata atgcacaatg gaaccatcaa
     2641 gaaggattgc gctcaacgat tactttccga attttgccag tatcgctttc actcgagttg
     2701 tcagctgatc gggagcaaac ggcttcagca gatattcctc tgctccagca tccagccctt
     2761 gttggatttc agtttcctgc ccgcgagccg tgaggaaaac caccgggata tctttcagat
     2821 cggggtttgc cttcatcact ttgcaggctt cgtacccggt catgcgtggc atgcgaacat
     2881 ccatgaggat caggtccggg tttaccttgg gtgccatctc aactgcctct tcaccgtttg
     2941 tagccgcaaa tacttcgtat cctgcaaaac gcaatgtaaa agccaccagg tcgcgtatat
     3001 ctcgttcatc ttctgcaatt aaaatttttg ccattcattc ctcattgttt ttcgtataca
     3061 ggcagcgtga aggaaaaggt gctaccgtta ccgggcacac cattactctt catccagatg
     3121 cggccctgat gcatttccac gatttgttta acgatggaaa gacccagtcc agttcccggc
     3181 gttgccagca ccagggggtg ttctccgcga tagaaccgct caaagacgcg atcctgatcc
     3241 tccagtgaga taccaacgcc gttgtcctgc acatccacct ggatctcatg tccaccattc
     3301 aacgggtgaa tatgaacggt gatggtccca ttctccggcg tataatgata ggcgttatcc
     3361 accagattcc ccatgatctg ccgaacgcgc tccacatctc catagactgc tggaagtttt
     3421 ttcggcgcat ccagggaaag cgccatcggc ttattctctt cctgtgaacg cctcagtacg
     3481 tcaccgatca catcttcagc aatttcgcgc agatcaagtg cctgtggcga aagagtgact
     3541 ctccccgact caatgcggga aatatccagc aaatcattca ccagaatgtt gagtctttcg
     3601 gtattatttt taacaatatt taggaagtga gtctggttct cattcatcgc gccagcggct
     3661 cccatcagga gcacatccac gtagccgcgg atggacgtca tgggcgtacg aagttcatga
     3721 ctcaccgtgg ctacgaattc agacttaagt cggtccacct cgacctcgtg ggtaatgtct
     3781 cggaaaatgg acacggtacc caaaaagtca ctttgaagga tcacgggggc aagatgcacc
     3841 agaatgattt gaccattttc caggtcaatt tgttccgcaa atgtatcgcc ctgctgatat
     3901 gccgcaggcg agtccgacca attctgaatg gtctgcatcc acggaccagc agatttaccg
     3961 aacaacccgc caaacacatc cagcgactgt ccgatgacgc gacccgattc gaggctgag
//
LOCUS       DASTJP010000091         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_951148, whole genome shotgun sequence.
ACCESSION   DASTJP010000091 DASTJP010000000
VERSION     DASTJP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951148"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VFQ23_02810"
     CDS             <1..1090
                     /locus_tag="VFQ23_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HET9905536.1"
                     /translation="VDYVTASLWRSSRLVNQERLLPTITLAMTLANFMSQELIVVIAL
                     ALIFDLLNGMRDASNIVATMISSRAFGPRTALGIAAIAEFLGPFLFGVVVAKTIGDEI
                     VQSSVLTLGVIAASLVGAIAWNLITWFFGIPGSSSHALIGGIVGAVLASAGMDAIKLA
                     GLNKVLIALFASPLIGFILGFILTRIIYFLVQNATPRINEFFKRSQLVTAIILALSHG
                     TNDAQKTMGIISLSLVIGGILPSFQVPLWVVFASAAGIAIGTSLGGWRLIRTLGGKFY
                     KIRPLHSFSTQLTSGIVMLTASLFGVPVSISQVVSSAIVGVGSAERVSKVRWSVAGDI
                     MTAWLITIPASALMGAGCYLLLRAIFAV"
     gene            complement(1087..2304)
                     /gene="serB"
                     /locus_tag="VFQ23_02815"
     CDS             complement(1087..2304)
                     /gene="serB"
                     /locus_tag="VFQ23_02815"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HET9905537.1"
                     /translation="MQEPILISITGMDRPGITSSVTQVLSEFDVDILDIGQSVIHDSL
                     SLGMLVKLPAHVSEETVIEAVQAAVDKLGLTIRFVKVSRESYADWVSQRGKGRHVVTL
                     LAKRITARHISQLAQIIAAHNLNIDKITRLSGRVSLDSIGNGGQACVEFSIRGQVADL
                     PALRREFMELADKIDVDIAYQENNVYRRNRRLVAFDMDSTLIQAEVIDELAKRAGVGS
                     QVAAITERAMHGEIDFRESLRQRVCLLKGLPESALAEVAATLKVTEGTERLMSTLKKL
                     GYKTAILSGGFTYFGRALQRRFGFDYVFANEPEIENGKLTGNLASEIVDGQRKASLLV
                     SIAQHEGFSLEQTIAVGDGANDLEMLSIAGLGIAFHAKPIVKESAKQSLSTLGLDSIL
                     YLMGLSERDTTTS"
     gene            2432..3373
                     /locus_tag="VFQ23_02820"
     CDS             2432..3373
                     /locus_tag="VFQ23_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905538.1"
                     /translation="MTKLFFATDIHGSDICWSKFLNAGKFYEADVLILGGDMTGKAVV
                     PFVHQGGHNYKVNLLEQDFPITNDDELAEMKKRVRSRGYYPYLTTPDEIAELEKDPDL
                     VDKIFLQEVLKVVQQWMDLADKKLDGTDMKVYCCPGNDDMDEVDAILRASKRVIHAEG
                     QVISLDDQHEMIASGWSNRTPWDTHREEDEDQLKVRYETMISKLKDPRNSVFNVHVPP
                     YKSGLDDAAELDKDLRPVLAGQSLQPVGSTALREAIQKVQPLLSLHGHIHEGRGATHI
                     GKTLCINPGSMYEQGTLLGAIVKLGKGKIENYVLTTG"
     gene            3450..>4276
                     /locus_tag="VFQ23_02825"
     CDS             3450..>4276
                     /locus_tag="VFQ23_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9905539.1"
                     /translation="MSNLFVRKATGMVRSWSVMDAFIYAFFSINLVTLGFYSFSQMYY
                     FGGGMIPALIVSAAFIIFEVVVYASLIAVMPRSGGDYVWQTRIFGGSIGFILAITGWW
                     FTLWLWTPIYGDMLRQIVLTPLLGAFGLQQAAVWIAGPDTALFVCSLLTLAFVALVIF
                     MGMKTYARIQKYSFYAGMLGLLIVIVLLFTGSPDRFKAGMESNSAAYFGTQAGVHDAT
                     AEAGVAAGAGTPLMGGSFLLIMLTLPYLVFFNLWPNWGATLYGEVRGATDYKRNFAG"
BASE COUNT          994 a         1028 c         1123 g         1131 t
ORIGIN      
        1 agttgattac gtcactgcga gcctttggcg aagcagtcgc ctggtcaatc aggagagatt
       61 gcttccgact atcaccctcg caatgacact tgcaaatttt atgtcccaag aactgattgt
      121 tgttattgcc ctggctttaa tttttgattt actgaacggg atgcgcgatg cgagcaatat
      181 cgtggcgacg atgatttcct cgcgggcgtt tggtccacgg accgcgctgg ggattgctgc
      241 cattgcggaa tttctcggtc cgtttttgtt tggcgtggtt gtagcaaaaa cgattgggga
      301 tgaaattgtt caatccagcg tgctgactct tggggtgatc gcagcgtctc tggtgggtgc
      361 cattgcatgg aatctaatta cgtggttctt tggcattccg ggtagttcat ctcatgcgct
      421 gatcggcgga atagtgggcg ccgtgttggc aagcgccgga atggatgcga tcaagcttgc
      481 aggtttgaat aaggtgttga tcgctctgtt tgcgtcgcca ttgatcggtt ttatacttgg
      541 gtttattctc acgcgcatta tttattttct ggtgcaaaac gccactccgc gaatcaatga
      601 atttttcaag cgaagccagt tagtgacagc catcattctc gccctcagtc acggcacaaa
      661 cgatgcccag aaaaccatgg ggatcatctc attgagcctg gtgattggcg gtatattgcc
      721 ctcttttcaa gtgccattat gggttgtgtt tgccagtgcg gcagggattg ccattggaac
      781 aagcctgggc ggctggagat taattcgcac attaggcggc aaattttaca agattcgccc
      841 tctgcatagt ttttcgacgc aattaacctc aggcatcgtt atgctcacag cctctctgtt
      901 tggcgtgccg gtaagcattt cgcaagtggt cagttcagca atcgtcggag taggctctgc
      961 agaacgcgta agcaaagttc ggtggagcgt ggcaggcgac atcatgacag cctggctgat
     1021 cacgatccct gccagcgcgt tgatgggcgc gggatgttat ttgctgctgc gagcaatttt
     1081 tgcggtttag gaagttgtcg tatctctttc cgagagaccc attaagtaca gaatactatc
     1141 caacccaagg gtactaaggg attgcttggc tgattccttg acgatcggtt tcgcgtgaaa
     1201 tgcgattccc aggccggcta tgcttagcat ctccaggtca ttagcgccat caccaactgc
     1261 aatcgtttgt tcaagtgaga agccttcatg ttgagcaata cttactaaca gagaagcttt
     1321 tcgttgacca tccacgattt ctgaagcaag gttgccggta agcttcccat tttcaatttc
     1381 aggctcgttg gcaaaaacat agtcaaagcc aaaccgcctt tgcagggcgc gaccaaaata
     1441 ggtgaaaccg cccgaaagaa tggcggtttt atagccaagt tttttcaggg tggacatcaa
     1501 gcgctcggta ccctcagtta ccttaagcgt ggctgctacc tccgccagtg ccgattcagg
     1561 cagccctttc aacaagcaaa ctcgttgcct cagactttct ctgaaatcaa tttcgccgtg
     1621 catggcgcgt tcggtaattg ccgcgacctg gctgccgacc ccggcgcgtt tggcgagttc
     1681 atcaatcact tctgcctgga tcaatgtgga gtccatatcg aatgccacca ggcggcggtt
     1741 gcgacgatag acgttatttt cctgatacgc aatatcgaca tcgattttat cggcgagttc
     1801 catgaattcg cgccgaaggg caggcaggtc tgcgacctgt ccgcgaatcg aaaattcaac
     1861 gcaggcttgc ccgccattgc ctatagagtc taaagagact ctgccagaga gcctggtgat
     1921 tttgtcgatg tttagattat gcgccgcgat aatctgtgcc agctgactga tatgacgagc
     1981 agtaattcgc ttggcaagca gtgtgacgac gtggcgaccc ttgcctcgct ggctgaccca
     2041 atcggcatag ctttcgcgac taacctttac aaaacggatc gtcagcccga gcttgtccac
     2101 agcggcttga accgcctcga tgacggtctc ttcgctcaca tgcgccggaa gttttaccag
     2161 cattccaagg gacaacgaat catgaatgac agactgacca atatccaata tatcgacatc
     2221 aaattccgac aatacttgag tcaccgatga cgtgatgccg ggtcgatcca taccggtgat
     2281 acttataaga atgggttcct gcaacgttac ctccgagttc cggggagaca ttttacatca
     2341 aatcagggaa ttcttactgc ggaggtataa ttgatttgta actacaacaa ttttatactt
     2401 cagcgtaact tcgcgaaaga aggatgtgtc tgtgacaaaa cttttctttg caacagatat
     2461 tcatgggtca gacatttgct ggagcaagtt tttgaatgcg gggaagtttt atgaggcgga
     2521 cgtactcatt ctcggcggcg atatgaccgg caaggcggtt gtaccatttg tccatcaggg
     2581 cgggcacaac tataaggtta acttactcga acaggatttt ccaatcacaa atgacgatga
     2641 actcgcagag atgaagaagc gcgtgcgaag ccgcggctac tatccctacc tcaccactcc
     2701 cgatgaaatt gcggagctcg aaaaagatcc tgatctggta gacaaaattt tcctgcagga
     2761 agttttgaaa gtggtgcaac aatggatgga tctggcggat aaaaaactgg atggcacgga
     2821 tatgaaagtc tattgttgtc cgggcaatga cgatatggat gaagtagatg ccatcctgcg
     2881 cgccagcaaa cgtgtgatcc acgcggaagg gcaggtcatc tctctggatg accagcacga
     2941 aatgatcgca tccggttggt ctaaccgcac accgtgggat acacatcgcg aagaagacga
     3001 ggatcagctc aaagtccggt atgagacaat gatctccaaa ctcaaagatc cccgcaactc
     3061 ggtgttcaac gtccatgtgc cgccgtacaa atcaggactg gatgatgccg ccgaattaga
     3121 taaggatttg cgacctgtgt tggcagggca atcgctccag cctgtaggtt cgactgcatt
     3181 gcgtgaggcg attcaaaaag ttcaaccttt gctcagcctg catgggcaca ttcatgaagg
     3241 gcgcggagcc acacacatcg gcaaaacgct ttgtatcaac cctggctcca tgtacgaaca
     3301 gggtacgctt ctgggggcga ttgtgaaact tggtaaggga aaaatcgaaa attacgtgct
     3361 cacaacagga taactctctt gacagttacg ggttgttaat cttggatgtt cgactgcccg
     3421 atcaaaatat cgatagagga gaagagatta tgagcaactt gtttgtccgg aaggcgaccg
     3481 gcatggtgcg gtcctggtcc gtcatggacg cgttcattta cgcgttcttt tcgattaatc
     3541 tggtcacgtt aggattttat agctttagcc agatgtatta tttcggtggc gggatgatcc
     3601 cggcgttgat tgtcagtgcc gcgttcatta tctttgaagt tgtcgtgtat gcctcgttga
     3661 tcgctgtcat gccccgctct ggtggtgatt acgtgtggca gacgcgtatt ttcgggggga
     3721 gcatcggctt catccttgcc atcacgggct ggtggttcac cctctggctg tggacgccaa
     3781 tctatggtga tatgctacgt cagattgtgc tcacaccttt acttggcgca ttcggtctac
     3841 aacaagcagc agtgtggatt gcaggtccag atacagcatt gttcgtttgt tctctgttga
     3901 cacttgcttt cgtggcgcta gtcatcttca tgggcatgaa aacctatgcc cggattcaaa
     3961 aatactcctt ctatgcaggg atgctcggat tattgatcgt gatcgtcctg ttgttcaccg
     4021 gctcccccga ccgattcaaa gcaggcatgg aatccaattc ggcggcctat tttggaactc
     4081 aagccggtgt gcatgatgcc accgccgaag cgggtgttgc cgcgggggca ggtacacctc
     4141 tcatgggtgg ctcgttcctg ctgatcatgt tgactctccc atatttggtc ttcttcaact
     4201 tgtggccgaa ctggggagcg acactatacg gtgaagtgcg cggcgcgacc gattacaaac
     4261 gcaactttgc cggaat
//
LOCUS       DASTJP010000092         9930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_331967, whole genome shotgun sequence.
ACCESSION   DASTJP010000092 DASTJP010000000
VERSION     DASTJP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331967"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFQ23_02830"
     CDS             complement(<1..725)
                     /locus_tag="VFQ23_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905540.1"
                     /translation="MQSRSLSILLPLGLFVLFLLGMALIQFATPDMPDNDGFYHIKLA
                     QLMRLDGLKPVFPWLPLSILNQNEFYDHHFLFHVALIPFTFGDLRVGAKWAAVIFASL
                     AFLAIWYLFHRRRVSFSWLWALALLGISDAFLYRMSITRAQSLSLAMLAIAFAMLLES
                     KHKHLAVLSFLYVWLYDAFPLILALAGLHLVSIAIIEHRLELRPLLYITGGILLGLVI
                     NPYFPENLIFSYHHMLPKLTDATS"
     gene            complement(816..1493)
                     /locus_tag="VFQ23_02835"
     CDS             complement(816..1493)
                     /locus_tag="VFQ23_02835"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HET9905541.1"
                     /translation="MKNTPRFLLFLLTLSVILLGACSSVPSPSSGDDANVNNSGITPV
                     VADDNGNSPSDSNSNDTNTNMNTNSNDNSNSSDIEVFGVVESMTETTITINGVTYNIV
                     ADLTQFNEVISVGDQVKISVIVNADGTFAIREIEFSLGNDNGNGNSNTNANSNDASNN
                     NSNTNDDNSNDDNGNSANSNDDNSNDDSGNDNGGNDNDNDSSGSGSGGNGNDDNGGND
                     NEDNDNG"
     gene            complement(1773..2123)
                     /locus_tag="VFQ23_02840"
     CDS             complement(1773..2123)
                     /locus_tag="VFQ23_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905542.1"
                     /translation="MKKRRIVVVSLILLVLIAGCAPGSSVEVTTPETNVQLTTPGVNP
                     LVNEPAENGVVAGVFQGLWHGIIAPVTLVLSFFNESVQMYEVHNNGREYNLGLLLGVA
                     LIFLILGFSGRRWR"
     gene            complement(2240..3349)
                     /locus_tag="VFQ23_02845"
     CDS             complement(2240..3349)
                     /locus_tag="VFQ23_02845"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET9905543.1"
                     /translation="MSILPSANETNPFIFVGTYTDPEGSKSEGIYVYRMDPSSGQLSL
                     VNVIKGVVNPSFLAVHPNQNFLYAVTEVDNLDGQARGALSAFSIDHNSGALALLNSQP
                     SHGTHPCYVSVEHTGRFALVANYTSGSVAMFPIHEDGALGEASDVVQHSGSSLHPSRQ
                     TSAHAHYIYPDPANRFAVAVDLGIDKLLTYRMDLEKGKLQLHGETGVHAGAGPRHLAF
                     HPNGTFAYLINELDATVITYRYHNEVGSFEQLQVLPALPKDFTSENLCADIHVTPDGS
                     FLYASNRGHESIVAFQIDQSTGLLTYINHTATGGREPRNFAIDPAGNFLLAANQKTDT
                     IFTFKIDPANGQLLRTGYQVEVSMPVCVKFVHLKN"
     gene            complement(3356..3760)
                     /locus_tag="VFQ23_02850"
     CDS             complement(3356..3760)
                     /locus_tag="VFQ23_02850"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HET9905544.1"
                     /translation="MDITLTTPGLLFPAVSLLLLAYTNRFLTLATIIRSLHDRYHASR
                     DENLLHQIDNLRYRIRLIRNMQIYGVLSLIFCVISMLALFAGWLVAGQWSFAIAMLLM
                     IASLAISLRELQISGGALDLLLKDMEEHRKTR"
     gene            complement(3785..4309)
                     /locus_tag="VFQ23_02855"
     CDS             complement(3785..4309)
                     /locus_tag="VFQ23_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905545.1"
                     /translation="MYILKPNERTMPVMVYTQDTLVRGEVIVPDSVQRVNIWLRMDGA
                     PHYMHILKPQVIVFGGSPLKPLSYSELFFPTTQVIAFHTIPPTDEPVDFDLTETDRMM
                     QTVDLLVGTFVMRGSTRISTHTELATSIEMTRVAWMSLYDVEISNPYLPQMPVLQVPM
                     VLVNSSHAAIGLES"
     gene            4422..6137
                     /gene="recJ"
                     /locus_tag="VFQ23_02860"
     CDS             4422..6137
                     /gene="recJ"
                     /locus_tag="VFQ23_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET9905546.1"
                     /translation="MHNKRWVIAPPITDQAEEALIKFPPILRQILFNRGLGSYEEARA
                     FLEARSALNHDPFQLTGMQTAVDRIRLALEHQEPIAVYGDYDVDGVTATALLVQALEM
                     LGANVRAYIPNRFDEGYGLNPDALDSLKADGVKLVITVDCGIRSPNEALHAQNIGLDL
                     IISDHHHPDGDNLPPAFAVINPKQHGDSYPDKDLAGVGIAYKLAEALMGNGNAHLTNG
                     QFHLFELLDLVALGTVADLAPLVGENRTLVRKGLRQIRQTKRQGLFSLAGVAQMKIDK
                     TTAGNIGFMLGPRLNASGRLESALASLELLTTKDFMRAGQLAQQLDVQNRQRQTITKN
                     MQEQAEQIAMSEDPNAFLLFAAHEDFNPGVVGLAASRLTETYYRPAVVAAKGTDETRG
                     SCRSIPEFHITDALDLCKDLLVRHGGHAAAAGFTVKNENLPALVARLKSIAEDQLSGK
                     DLRQTLSADMVVPLSDLNFEVLKYLAYLEPTGYGNPDAIFVSRNVKVKSSRMVGAEGR
                     HLKLTLEDERGASIDSIGFRMGHLQLNLPPRVDVLYHFESNEYNGRTSLQLNLKDVKP
                     AGTPD"
     gene            6228..6791
                     /locus_tag="VFQ23_02865"
     CDS             6228..6791
                     /locus_tag="VFQ23_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905547.1"
                     /translation="MNSQSNYLEKPTLVNVIAWMTLASGVVNLVWGVAASGTVLATVV
                     GVVCVPFTILPTLLGVFELIYAAKLFSNPAQPVQPSSYIAVFEIACVLTGNVFAMIVG
                     ILALVFYNDTIVKDYFARLNGLIPGGGGMPAPIVPAVPVTPASSMSEAEAPVVPEPPA
                     PPTDESLTSSTDESSSKPKRTRKTTNE"
     regulatory      6893..7045
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            7273..7650
                     /gene="gcvH"
                     /locus_tag="VFQ23_02870"
     CDS             7273..7650
                     /gene="gcvH"
                     /locus_tag="VFQ23_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9905548.1"
                     /translation="MNTPANLKYAKSDEWFDSESGKIGITDYAQSQLSDIVFVEVLVD
                     EGETIQAGKPIASVESVKASAEIYSPASGTVIAVNKDLPNKPEALNSDPYGAAWMIQL
                     EGGSEGDLMDSAAYEKHCEERDH"
     gene            7733..9085
                     /gene="gcvPA"
                     /locus_tag="VFQ23_02875"
     CDS             7733..9085
                     /gene="gcvPA"
                     /locus_tag="VFQ23_02875"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET9905549.1"
                     /translation="MTYIPISPKERDSMLETIGVKSLDDLFEDVPEKHRFPELNLPPA
                     LTEMEAMAELSNLANSNEHVRGELISFLGAGMYNHYIPSVVDHMLRRGEFYTAYTPYQ
                     PEISQGTLQAIFEYQSLMSALTGMDVSNASHYDGATATAEAVSLAYAQFRGKRKKVVM
                     SPGVHPQYRAVVRTYAQAMGMETAGNEESGVGTNPDVLANRVDENTCLLIIQYPDFFG
                     RIFDYTKLIDSAHTKGALVCIVANPTALALYKTPGEMGADIVVGDGQPLGIPMWFGGP
                     SVGFFTTRKQYVHKMAGRLVGETIDNRGQRAYVLTLTAREQHIKRERATSNICTNQGL
                     LALATAVYLSLLGKSGLRQVAELCYQKAHYAAREITKLGDFRITFKEPFFHEFTLECP
                     KPASAINAYLLEHGILGGYDLGQDYPPLKNHLLVAVTEMNSKEQIDTLVEVLSEVSHG
                     "
     gene            9078..>9930
                     /locus_tag="VFQ23_02880"
     CDS             9078..>9930
                     /locus_tag="VFQ23_02880"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET9905550.1"
                     /translation="MVDVLPDTQVKAAPKIIEPTIFDLSSPGRKGVKMPASDVPTIDL
                     PPKNLLRSELPLPELAEVDVVRHYMHLSKFNYSVDSGFYPLGSCTMKYNPKINEDTCR
                     LPGFLFTHPLQPVETVQGNLVVMYQLQEWLKEIAGFAAVTLQPAAGAHGEFTGVLMMR
                     AYHNSRGDNKRTKMLIPDAAHGTNPASVGMMGMSVVTIPSDSRGNVDLQALEAACDDT
                     VVGMMLTNPNTLGLFDENIERVIELVHGCGGLMYGDGANMNAMLGIVRPGDLGFDIMH
                     FNLHKTFS"
BASE COUNT         2641 a         2460 c         2394 g         2435 t
ORIGIN      
        1 gaggtggcgt ctgttagctt gggtagcata tgatgatatg aaaagataag gttttctgga
       61 aagtatggat tgatcaccaa gccaagcagg attccgcccg ttatgtaaag cagcgggcga
      121 agctccaggc ggtgttcaat aatagcaatt gagacaagat gtaacccagc cagagccaga
      181 attagaggaa aagcgtcata cagccatacg tacaaaaaag ataaaacagc cagatgcttg
      241 tgcttgcttt caagcagcat tgcgaatgca atcgccagca tcgccagcga aagagattgg
      301 gcgcgtgtaa tgctcatgcg atacaaaaat gcgtcagata ttccaagcaa ggcaagcgcc
      361 cacaaccagg aaaatgaaac gcgtcggcga tgaaagaggt accagattgc cagaaacgcc
      421 aggctggcga aaatcaccgc tgcccacttg gcgccgacgc gcagatctcc aaacgtaaac
      481 gggatgaggg ctacatggaa aaggaaatga tgatcgtaaa attcattctg attgagaatg
      541 ctcagcggta gccaggggaa aaccggcttg agtccgtcca ggcgcatcaa ctgtgccagt
      601 ttgatgtgat agaagccatc attatcgggc atatcgggcg tggcgaattg aatcagcgcc
      661 atgccaagca ggaacaggac aaagagtcca agggggagaa ggatggataa tgaacgactt
      721 tgcacaggaa gattttatac tccaaatgga ctggttaccg aagatctgta cagattatcg
      781 gtaaccagcc aattcatctc ggttgctaac aacaactatc cgttgtcatt atcttcatta
      841 tcattcccgc cattgtcgtc attgccattg ccaccagagc cggagccgct gctgtcatta
      901 tcgttatcgt ttccaccgtt atcgttcccg ctatcatcgt tggagttgtc gtcgttggaa
      961 ttggcgctgt tgccgttatc atcattgctg ttgtcgtcat ttgtgttgga attattattg
     1021 ctggcatcat tcgaattggc gttggtattt gaatttccat tgccgttatc atttccgagc
     1081 gagaattcga tctcacgaat ggcaaaggta ccatccgcgt tgacaattac actaattttg
     1141 acctggtcac ccaccgaaat cacctcattg aattgtgtca gatctgccac aatgttgtag
     1201 gtcacgccgt tgatcgtgat agtcgtttcc gtcatcgatt ccacaacacc gaacacttct
     1261 atatcagagc tgttcgaatt atcgttgctg ttcgtgttca tattggtgtt ggtatcattt
     1321 gaattcgagt cgctggggct gttcccattg tcgtctgcaa caacaggagt gatacccgaa
     1381 ttattcacat tagcatcatc tccgctggat ggtgaaggca cgctgctgca tgcgccgaga
     1441 agaatgacag acaaagtcag aagaaaaagg agaaatcggg gtgtattttt catgaggtac
     1501 ctctcttgat ttgaattggt ttcacttagt agtcgtcagg caacaaaaaa agttacgggt
     1561 tgactgatgg aggatttttt tatgagtctg gccatattgt gaattttatc acaataaaca
     1621 gcttttggat agcttgccag atccgttcag acaacaatgc ttgttgggcc cgtgaaaaca
     1681 aaacctccca aggtggctcg ggaggcagta acatttcggg aaaatacgtt caaactggcg
     1741 ggttttccgc ctgacgcttt atgtagttat atctatctcc accggcgccc cgaaaaacct
     1801 aaaataagaa atatgagcgc cacgcccagc agtaaaccaa ggttgtattc tcttccgttg
     1861 ttatggacct catacatctg tacgctctca ttaaagaacg acagaaccaa agtcactggc
     1921 gcaataatcc catgccaaag tccttgaaat acccccgcca ccacaccatt ttcagccggc
     1981 tcatttacga gtggattgac acccggtgtg gtaagttgca cattcgtctc tggtgtcgtg
     2041 acttcgacgc tggaacctgg tgcacagcca gcaattaaaa caagcaaaat caacgatacg
     2101 accacaatac gtcgcttttt catctctgcc tcctcattaa ttacagcttt caattatctg
     2161 tactatttaa cgaggtgttc ggggattcat tatgcgactg cactatggaa agaaatcctg
     2221 acattcccct ctattaatat caatttttca aatggacaaa tttcacgcag accggcatcg
     2281 atacttccac ctggtaacca gtcctaagca attgaccgtt cgccggatca atcttgaacg
     2341 tgaaaatcgt atctgttttt tgattagcgg cgagtaaaaa gtttccagcg gggtcaatgg
     2401 cgaaattgcg aggttcccgt ccgcctgtcg ctgtgtgatt gatataggtc aataagccag
     2461 tgctttgatc gatttgaaaa gccacaatgc tctcatgccc gcgattggag gcatacagaa
     2521 aacttccatc cggcgtgaca tgtatatctg cacacagatt ttcgctggtg aaatctttgg
     2581 gcagtgccgg gagaacctga agctgctcaa aactgccaac ttcattgtga tatcgatacg
     2641 taatgacggt cgcatccaat tcgttgatca gatatgcaaa cgtaccgttg ggatggaacg
     2701 ccaggtgccg cggtcctgcg ccggcatgca cacccgtttc gccgtggagc tgcagcttgc
     2761 ctttttcaag atccattcga taggtcagca gtttgtcgat accaaggtca actgcaacgg
     2821 caaagcgatt ggctggatcc ggatagatat agtgagcatg tgcgctcgtt tggcggctgg
     2881 gatgaaggct cgagcctgaa tgctgaacta catcactggc ttcgcccaga gctccatctt
     2941 catggattgg gaacatggca acacttccgc tggtatagtt cgccacgagt gcaaatcggc
     3001 ccgtgtgttc aacactcaca taacagggat gcgttccatg tgacggttga ctgttcagca
     3061 gcgccaatgc tccagagttg tggtcgatgg aaaacgcgct caaggctccc ctggcttgtc
     3121 catcgagatt atccacctcg gtcacagcat aaagaaaatt ctgatttggg tgaacggcaa
     3181 gaaaagaggg attaactaca cccttgatta cattaaccag actgagttgg cccgagctag
     3241 gatccatccg atagacataa ataccctcac ttttactgcc ctcggggtcg gtataagtgc
     3301 ctacaaagat aaacggattc gtttcattcg cagagggcaa aatagacata tacagttacc
     3361 tggttttcct atgctcttcc atatccttta gcaaaagatc cagcgcgcca cccgagattt
     3421 ggagttcgcg caatgaaatt gccagtgaag cgatcatcag gagcatcgca atcgcaaagc
     3481 tccattgccc ggccactaac cagccagcga acaaagccag catgctgatc acacaaaaga
     3541 tcaaacttaa gaccccataa atctgcatat tgcgtatcag gcgaattcga tagcgcagat
     3601 tatcgatctg gtgaagcaaa ttttcatcgc gactggcatg ataccggtca tgcaacgagc
     3661 gaatgatggt cgccaaagtg agaaaccgat tggtatacgc cagtaacaat aatgaaacag
     3721 cagggaataa taagcctggt gttgtgagag tgatatccat attgtgaagt tatattttca
     3781 caacttatga ctccaaacca atcgctgcgt gactggagtt cactaagacc atcggaactt
     3841 gcaaaacggg catttgtggc aaataggggt tggatatctc cacatcatac aaagacatcc
     3901 aggcgacgcg cgtcatctca atgctggtgg cgagctctgt gtgagtcgaa atgcgagtac
     3961 tccccctcat cacaaatgta cccaccaaga ggtccaccgt ttgcatcata cggtcggttt
     4021 ccgtgagatc aaaatccacc ggttcatctg tgggtggtat ggtatgaaaa gcaatcactt
     4081 gtgtagtcgg aaaaaatagc tcggagtaac ttaacggttt cagcgggctt ccgccaaata
     4141 caattacctg gggtttgagg atatgcatgt aatgaggcgc gccgtccata cgtagccaga
     4201 tatttacgcg ctggacagaa tcgggaacga taacttcccc acgcaccagt gtatcctgtg
     4261 tataaaccat gacgggcatc gtgcgttcgt taggtttgag aatgtacatg actcctccag
     4321 tgactattat tatacacttg caggcttgtc caatcacagt ttatactatg tctgacaaat
     4381 tttgttttta tgtacactca agagagaagc ttaagtaata tatgcacaat aaacgctggg
     4441 taattgcccc gccgataacc gatcaagccg aagaagccct gatcaaattc ccgcccattc
     4501 tcagacaaat cctttttaac cgtggtctgg ggtcttacga agaagcacgt gccttcctgg
     4561 aagcgagatc cgctctcaat cacgatccat ttcaactgac tggaatgcag acggccgtgg
     4621 atcggattcg cctggcgctc gaacatcagg aaccaattgc cgtttacgga gattacgatg
     4681 tcgatggcgt caccgctacg gcattactcg tacaggcgct cgagatgctg ggtgcgaacg
     4741 tccgcgccta tattccgaat cggtttgacg aagggtatgg gctgaatccg gacgccctgg
     4801 attcactcaa agcggatggc gtcaaactgg tcattactgt tgactgcggc attcgttcgc
     4861 caaacgaagc acttcatgct cagaacatcg gcctggacct cattatctcg gaccatcatc
     4921 accccgatgg cgacaacctg cctcccgctt tcgcggtgat aaaccccaag caacatggcg
     4981 acagctatcc tgataaggac ctggctggcg ttggcattgc atacaaactc gcagaagccc
     5041 tgatgggaaa cggaaacgcc catcttacta acggacagtt ccacctcttc gaattacttg
     5101 atctggttgc cttaggcacc gtcgcggacc tggctccatt ggtcggcgaa aaccgtacac
     5161 tcgtccgcaa gggactcaga caaattcgcc agaccaaacg gcagggactc ttttccctgg
     5221 ctggcgtggc gcaaatgaag atcgataaga cgactgcagg taacatcggc tttatgctgg
     5281 ggccacgatt gaatgcctcc ggtcgacttg aatcggcgct ggcctcgctt gaactgctta
     5341 ccacaaaaga ctttatgcga gctggccaat tggcacagca acttgatgta caaaatcgcc
     5401 aacgacaaac gatcacaaag aatatgcagg aacaggcgga acagatcgct atgtcagaag
     5461 atccaaatgc atttctactg tttgctgcgc atgaagattt caaccccggc gttgtagggt
     5521 tggcggcctc tcgattaaca gaaacgtatt accgccccgc ggttgtcgcg gcaaaaggca
     5581 cggatgaaac acgcggttca tgccgctcga tccctgaatt tcatattact gatgcacttg
     5641 atttatgcaa agatttgctc gtccgtcatg gagggcatgc cgcagcagct ggtttcaccg
     5701 tgaagaatga aaatctcccc gcattggtcg ctcgtctcaa atccatcgcc gaagaccaac
     5761 tctcagggaa ggacttgcgt caaacgctat ctgcggatat ggtggttcca ctttctgact
     5821 taaatttcga agtattaaaa tatcttgctt atctggagcc caccggttac ggtaacccgg
     5881 atgcgatctt cgtctcacgg aatgtcaagg tcaagtcctc gcgcatggtt ggcgcagaag
     5941 gcaggcactt gaaactgaca ctcgaagatg aacgcggtgc cagcattgat tctatcggct
     6001 tccgtatggg gcatcttcag ctcaacctgc ctccgcgggt cgacgtgtta tatcacttcg
     6061 aatcgaacga atataatggt cgcacatcat tgcaattgaa tttgaaagat gtgaagcccg
     6121 ccggcacacc tgattaactt ttttgtcgcc tgcaaccttt ctccaaaaat gacgtaggaa
     6181 gaagtgaaac tacctctgtg cgacagtcta tactttagga gaaagatatg aactctcagt
     6241 ccaattattt agaaaaacca acccttgtaa atgtcattgc gtggatgact ctcgccagtg
     6301 gcgtcgtcaa tttagtgtgg ggcgttgctg cctcgggaac ggtgcttgct accgtggtcg
     6361 gtgtcgtctg tgtgcccttc accattttgc cgactctgct cggcgtcttc gaactaattt
     6421 acgccgcaaa actgtttagc aatcccgccc aacctgtaca gccatcatct tatatcgcag
     6481 tttttgaaat cgcatgtgta ttgaccggga atgtcttcgc aatgatcgta ggtattcttg
     6541 cactcgtttt ctacaacgat acaatcgtca aagattattt tgccagattg aacggtctca
     6601 tccctggtgg aggcggtatg cctgcaccga tcgttccagc agtgccagtt acccccgcct
     6661 cttcaatgag tgaagctgag gcaccagttg tgccagagcc acctgcccca cctactgatg
     6721 aatctctgac atcttcaacg gatgagagtt cttcaaaacc aaaacgaacc cgcaagacca
     6781 caaatgaatg aaacaccaga cttcggaagc gccaaaaaac tggcaagact tccgaagtct
     6841 ttttactctt gactagcatc catctcgctc ataaaatgga tagcacaaca tagcggataa
     6901 cagggaagag actcaaaata gtgacgactt tagtcgtctg gaattaagcg actgaagtca
     6961 ctaccacaat tagagcgccg aaggtgcaag tctggaacag gcgaaaccag gcgaaactct
     7021 caggcaaaag gaccctgttc cgaatcactc tggaaagttc ctttgtgtat gatatttctg
     7081 gcaaaggata ccgacggtgt aaagttttag tctgctggtt tgatagtcta caagtccaca
     7141 caaaggacag gctgaataaa ctaaaggact attactaatc tctcaggttc aaccacagag
     7201 gcgcgcgatc catttcgtgc gcctttatcg tttaaaacga agcgccaaca caactttgat
     7261 aggagacttt ctatgaatac tccagccaat ttgaaatatg caaaatcaga tgaatggttc
     7321 gactcagaaa gcggcaaaat tggcattaca gattatgccc agtcgcaact ttcagatatc
     7381 gttttcgtgg aagtgttagt ggatgaaggc gagaccatcc aggcaggtaa gcccattgca
     7441 tcggtcgagt cagtcaaggc ttcggcagaa atttactcgc cggcctccgg gacagtcatt
     7501 gctgtgaaca aagaccttcc caacaaacct gaggcgctca atagtgatcc gtatggcgcg
     7561 gcgtggatga tccaacttga aggtggctcg gaaggtgatt tgatggattc cgctgcgtat
     7621 gaaaagcatt gcgaggaaag agaccattag tcaacccgtc gccgctcgaa tggtccgctt
     7681 gactttggcg gtagactaag taactaaagg actagaaaac aagaaaacta acatgacata
     7741 tatcccaatt agccctaaag aacgcgattc aatgctcgaa acgattggcg tcaaatcgtt
     7801 ggatgatttg ttcgaagatg tccctgaaaa gcatcgtttt cctgaactaa accttccgcc
     7861 cgcgctgacc gaaatggaag cgatggctga gctctcaaat ttggccaatt caaatgagca
     7921 tgtgcgcggc gaactgatct ccttccttgg cgcagggatg tataaccatt acatcccgtc
     7981 cgtggttgac catatgctgc gccgtggaga attttataca gcatacactc cctaccagcc
     8041 agagatttca caaggcacgt tacaagccat ctttgaatat caatctctga tgtctgctct
     8101 caccggcatg gatgtatcca acgcctcgca ctatgatggc gcgacggcaa ccgccgaagc
     8161 cgtcagcctg gcgtatgctc aattccgcgg gaaacgcaaa aaggtggtga tgtcccctgg
     8221 tgtgcatccg caatatcgcg cggtggttcg cacgtatgcc caggcgatgg gtatggaaac
     8281 agccggaaat gaggaatcag gcgtcggcac gaatcccgac gtgttggcga atcgagtgga
     8341 tgaaaataca tgtctgctta ttattcaata cccagacttc ttcggtcgga ttttcgatta
     8401 cacaaaactg atcgattctg cacacacgaa aggtgcatta gtatgtattg tggcaaaccc
     8461 aacggcactg gcactgtata agacccccgg agaaatgggt gcagatatcg tggttggtga
     8521 tggtcagccc ttgggaattc ccatgtggtt tggtggaccc agcgtcggct ttttcaccac
     8581 acgtaagcaa tatgttcaca agatggcagg tcggctggtc ggcgagacca ttgacaatcg
     8641 tgggcaacgc gcctacgtgc tcactttgac cgcgcgcgaa caacatatca aacgcgaacg
     8701 cgccacatcc aatatctgca cgaaccaggg attgcttgcg ctcgccactg cggtgtatct
     8761 ttcgctgctc ggcaaaagcg ggctgcgcca ggtggcagaa ctctgttatc aaaaagcaca
     8821 ttacgccgcg cgcgagatca caaaacttgg cgattttcga atcacattca aagagccttt
     8881 cttccacgag ttcaccctgg aatgtccgaa gccagcatct gcaattaatg cgtatttgct
     8941 tgagcacggc attctcggtg gatacgatct ggggcaggat tatccaccgc ttaagaatca
     9001 tctattggtc gcggtgaccg aaatgaacag taaagaacaa attgatacgc ttgtcgaagt
     9061 tttatcggag gtgagccatg gttgatgttc tccctgacac acaggtaaaa gccgctccga
     9121 agatcatcga acccaccatt ttcgatttat cttctccggg acgaaagggc gtaaagatgc
     9181 ccgcttcaga cgtgccgaca attgatctgc cgcccaaaaa tcttctccgt tccgagttgc
     9241 ctctgcccga actcgccgaa gtggatgtgg tacgtcacta tatgcatctc tccaaattca
     9301 actacagtgt ggacagcggc ttctacccgc tgggttcatg cacgatgaag tacaacccaa
     9361 aaattaacga agacacctgt cgtcttcccg gatttctctt tacacatccg ttgcaacccg
     9421 ttgaaacagt gcagggcaat ttggtcgtga tgtatcaact gcaggaatgg ctgaaagaga
     9481 tcgcaggctt cgcggctgtg accttacagc ccgcagctgg tgcacatggc gaattcaccg
     9541 gtgtattgat gatgcgcgct tatcataact cccgcggcga taacaaacgg accaagatgc
     9601 ttatccctga tgccgcgcat gggacgaatc ccgcctcagt cggcatgatg ggaatgagcg
     9661 ttgtgaccat tccctcggac tcgcgcggca atgtagatct gcaggcactc gaagccgcat
     9721 gcgatgacac tgtcgtcggt atgatgctta ccaacccaaa tacgcttggg ctctttgatg
     9781 aaaacatcga gcgagtcatt gagcttgtcc atggatgtgg tggcttgatg tacggcgacg
     9841 gcgcgaacat gaacgcgatg ttaggcatcg tccgcccggg tgacctgggc ttcgacatca
     9901 tgcacttcaa cctgcacaag accttttctg
//
LOCUS       DASTJP010000093         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1261039, whole genome shotgun sequence.
ACCESSION   DASTJP010000093 DASTJP010000000
VERSION     DASTJP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261039"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFQ23_02885"
     CDS             <1..230
                     /locus_tag="VFQ23_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905551.1"
                     /translation="LSAHAAKGEWTDLPMLISDEMLSEFCLVTEESKLAHDLKKRYEG
                     IANRLTPYLPFVPGERDERWRELTKEFNLHK"
     gene            282..755
                     /locus_tag="VFQ23_02890"
     CDS             282..755
                     /locus_tag="VFQ23_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905552.1"
                     /translation="MENSAEAMAVRFHDVEFSPQGITLLSQQSKLLYLAKQDIRKITL
                     KKGFQAERPIAEILFGIFVIGLGLYFFANFVLEALIHRTIYLDDLLSLFLLPIGGWFI
                     VDGLRKRLYFEVVLDRDTRKFPLGKNPDKGELGKFIRIATQLGYVIDARFLDQVD"
     gene            1104..2165
                     /locus_tag="VFQ23_02895"
     CDS             1104..2165
                     /locus_tag="VFQ23_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905553.1"
                     /translation="MLNQTNSSFTVTTPPSEARALVESFARQDQQRLYPFELQNFTLG
                     VRFQNADPKTQRVLVMAMLAWLDQHSLQSYGYQDQSAWQMGWKMREAFLHMLQFKIPF
                     QENDVIAMLNWSAVQASAPAYVYFSGVPQIIKVVGGYLKDHPLSDEVHESIERLAESL
                     EAVSMSVEIRRWLLRLKELMGETQVSLPLVAGDAWADTALKEIYAMNARAKTAWAELL
                     RHCSQASGSAPSRRWLAEADKYIDAIGDLYLFIKLSHWFPLADQPQASAANATILQGL
                     VWLCSKSEDPDIATALSGLAISSYEARTVKIGNACLWSLAHMPAREGITQLHILRDTF
                     TSQDLHRKISKALAAATRR"
BASE COUNT          706 a          608 c          636 g          632 t
ORIGIN      
        1 aactctcggc tcatgcagcg aagggtgaat ggacggattt gcccatgctc atctcagatg
       61 agatgctgag tgagttttgt ctggtgacag aggaaagcaa actggcgcat gacttgaaga
      121 aacgctacga aggaatagca aacaggctga caccatacct tccctttgtc cccggagaga
      181 gagatgaacg gtggagagag ttaacaaaag aatttaacct gcataaatag ctaccgactg
      241 gaactttatt cgttttgtaa ggaacagagg aagggtgatc gatggaaaat tctgccgagg
      301 caatggctgt aaggtttcat gatgtggaat ttagtcctca gggcataacc ttactgagtc
      361 agcagagcaa gttgttatat cttgccaaac aggacattcg taaaattaca ctcaaaaaag
      421 gctttcaggc agagcgaccc atagccgaga ttttgtttgg gatctttgta atcggattag
      481 gtctctactt tttcgccaac ttcgtgcttg aagcccttat tcacagaaca atatacctgg
      541 atgatttatt gagtctcttc cttttgccta taggaggctg gtttatcgtg gatggtttga
      601 gaaagaggct ttattttgag gttgtattgg accgcgacac acgcaagttt cctcttggca
      661 aaaaccctga caaaggtgaa ttggggaaat ttatcaggat tgcaactcaa ctgggatatg
      721 taattgatgc aagatttttg gatcaagtag actaagccca acagagtttc tgatcttcca
      781 tcgccgttca accggggaca acatcagcca gactcttcgc catgagaatt ttcaaagaat
      841 cttgacaggt aaagaaaaat ccatataatt agaacagatg ttctaaaacc agacaactga
      901 attagagtcc catgcttact gaacctttga tttgcgatga aacgatttcc agcctgggta
      961 aaacagacac actttgacta ttcatcatta cggggaaaca accgcctgac tctcgaaatc
     1021 gcttttcatc cagggacttc ccatgctttc agcacatttc tcagttacgt tccactcttt
     1081 cgtggataaa ggaagatgtc ctgatgttaa accagaccaa ttccagtttt accgttacca
     1141 ctcccccctc cgaggcaaga gctctggtcg agtcttttgc caggcaggat cagcaacggc
     1201 tttacccctt cgaactacaa aactttacgc tgggtgtacg atttcagaat gcggatccaa
     1261 aaacgcagcg tgtactcgtc atggcgatgc ttgcctggct ggatcagcac tcgctccagt
     1321 cctacggcta tcaggatcaa agtgcatggc aaatgggctg gaaaatgcgc gaagccttcc
     1381 tgcacatgct ccagttcaaa atcccctttc aggaaaatga tgtaattgcc atgctgaatt
     1441 ggtcggcggt gcaagccagc gcacccgcgt atgtctattt cagcggtgtg ccgcagatca
     1501 taaaagtggt cggcgggtat ctcaaggatc acccattgag cgatgaggtg catgagtcca
     1561 tcgagcgatt agccgaatcc ctggaagcgg tcagcatgag tgtcgaaatc cgtcgctggc
     1621 tgctgcggct gaaggaattg atgggtgaaa cgcaggtcag tttgccgctt gtcgccgggg
     1681 atgcctgggc ggatactgca ctaaaagaaa tctacgcgat gaatgcaagg gcgaaaacag
     1741 cctgggcgga gctgcttcgg cattgttcgc aggcgtcagg ttctgctccg agccggaggt
     1801 ggttggcgga agcagacaag tatatcgatg caattggcga cctgtatctg ttcatcaagc
     1861 tatcccattg gtttcctctc gcggatcagc cacaggcctc ggctgccaac gccaccatcc
     1921 tgcagggact cgtttggctg tgcagcaaat cggaagatcc cgatattgcc acggccctgt
     1981 cagggctggc gatctccagt tatgaagcca ggactgttaa gatagggaat gcctgtctgt
     2041 ggtcactggc acacatgcct gcccgcgaag gcatcacgca gctgcatatc ttaagagata
     2101 cgtttaccag ccaagatttg cacaggaaaa tttcaaaggc acttgcagca gcaaccagac
     2161 gataacgaaa ttttattccc cgagacttga cctcgtcaaa ccggagcctc cagaaccata
     2221 tgaattcgat gcaatatggg tttggaggtt ctgctatgcc aatcgcaaac aggttctcaa
     2281 cctcagccaa gggaaaccag cagttttaag cggcgggcca ggcagcattg tttatcaggc
     2341 tacgtgagac gcgatccata tgccgaataa cccatttggg ctactggaca ggcaaaaagt
     2401 tacaaccgta ttgactctcg tctaaaagat cctataattg aaacgtatgt tctaaaacca
     2461 aatacacgaa taggaggcac ccatgcttac caaacttttg atccgagatg aaacgacttc
     2521 cagcctgggt aaagcggacc acacgttcac agttcatgtg tcaggggaga aaatcagcat
     2581 cc
//
LOCUS       DASTJP010000094         4407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_647140, whole genome shotgun sequence.
ACCESSION   DASTJP010000094 DASTJP010000000
VERSION     DASTJP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647140"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFQ23_02900"
     CDS             <1..438
                     /locus_tag="VFQ23_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905554.1"
                     /translation="LQDNATPSGNALACEALLKLAAFTDDGNYRDLAEKSLGLSAVMA
                     AQYPTAFSRWLSAADFALSNVKQVAVVYESDKGQANELIQLVEAAYRPSVIVAASAYP
                     PSDDAPALFMDRPLKNGRATVYVCEGFVCKNPINTISELKELL"
     gene            448..1089
                     /locus_tag="VFQ23_02905"
     CDS             448..1089
                     /locus_tag="VFQ23_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET9905555.1"
                     /translation="MDTILIENLIKVGMAVVVGGLIGAEREYQDKAAGFRTIILITVG
                     STLFTIFSVSMDPGFTRTRVAANIVTGIGFLGAGAIVREHGKIGGLTTAATIWLAAAL
                     GMGIGAGELMFVSLSTVIVVIILLVFPRIESRIDRIRESRTYKIVVTSQNLEMTENIY
                     DSIRDCSLQVLEHHQSKTGETIIGTWRAIGSPENHEKFVGKMMRDKGVKELVY"
     gene            complement(1167..1361)
                     /gene="tatA"
                     /locus_tag="VFQ23_02910"
     CDS             complement(1167..1361)
                     /gene="tatA"
                     /locus_tag="VFQ23_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9905556.1"
                     /translation="MPGLPRGAELIVILVIVLLLFGPGRIGKLASELGKGIKAFRDGL
                     EKDKPENEASTDASGNIEPK"
     gene            complement(1424..2527)
                     /locus_tag="VFQ23_02915"
     CDS             complement(1424..2527)
                     /locus_tag="VFQ23_02915"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET9905557.1"
                     /translation="MPYAHITGWGMAAPESIMTNDDLSKLVETNDAWIRERTGIRERR
                     IARENEFPSTLGVEASIKALTVANLRPTDLDLIICTTSSPEYIFPATACLIQDQLGAS
                     KAGAFDLLAACSGFIYALNMGAQAIRSGSIKSALIVGSETLSRFINWKDRNTCILFGD
                     GAGAFVLQASDQPGGVLSAVMHSDGSGGDLLTIEGGGSRYPASEATIHTGRHFIQMDG
                     KEVFRFATRVMASATQEALHAAGLTIDEIRWIIPHQANIRIIEAAARGLKLPMDRFIV
                     NLERYGNTSTASIPIAMVEALEKGQIKQGDKLVMVGFGAGLTWGALAAEWTGPIPSKG
                     HVRPEQYRTFGRLRSFVRRALRFIEGLLYRREL"
     gene            complement(2581..3822)
                     /gene="fabF"
                     /locus_tag="VFQ23_02920"
     CDS             complement(2581..3822)
                     /gene="fabF"
                     /locus_tag="VFQ23_02920"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9905558.1"
                     /translation="MKRRVVITGMGCISPVGNNVAETWSAILAGKSGSDMITLFDASK
                     HKTRFAAEVKGFDGVALFGNREARKMDRFTQFAVAATGQALEQSGLKIDESNRDRVGI
                     VIGTGIGGIGTMLDQAEVMRERGPERVSPFLIPMMISDSAPGMLAIRVGARGPNMAIA
                     TACASGNNALGDALETIRRGSADAMIAGGSEASLVSLAMAGMNVMGALSTRNDDPKTA
                     SRPFDKDRDGFLMGEGAGILILESLEHATARGANILGELSGYGTTDDAHHISAPAENG
                     AGAAMSMKLALENAELTVNDIGYINAHGTSTPLNDKSETAAIKTVFGEQAYNIPVSST
                     KSMTGHLLGASGAVEAVFSIMAIRDGILPATINYQTPDPQCDLDYVPNEPRKVSPRHV
                     MSNSFGFGGHNATLIFSRFEE"
     gene            complement(4016..>4407)
                     /locus_tag="VFQ23_02925"
     CDS             complement(4016..>4407)
                     /locus_tag="VFQ23_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9905559.1"
                     /translation="DEEWRHEPTREDWAVNEIVCHLRDTEMEIHRLQLDLMLARSGAF
                     IPRPDTSVWANEREYLNEDGLTALADFTAARLANLLKLAGSDSELWSRRARHAIFGPT
                     NFLEVISFIADHDRMHIQQAWKTLNSL"
BASE COUNT         1105 a         1197 c         1028 g         1077 t
ORIGIN      
        1 ttacaggaca atgccacccc atccgggaac gctctggcgt gtgaagcgtt actaaagctc
       61 gcggcattca cagatgatgg aaactatcgc gatctggcag aaaagtcgct tgggctttcg
      121 gcagtgatgg ctgcacaata cccgaccgct ttctcgaggt ggttatccgc agcggatttt
      181 gctctgagta atgtgaagca ggttgcagtc gtgtatgaat cagataaggg tcaagcaaat
      241 gagttgatcc agttggtcga ggctgcatat cgacctagcg tgatcgttgc cgcatccgct
      301 tatcccccat ccgatgacgc acccgcgctt ttcatggatc gcccactaaa gaacggaaga
      361 gcaacggtgt atgtgtgtga aggtttcgtt tgtaaaaatc cgattaatac aatttcagaa
      421 ctaaaagagt tgctgtaaaa tcagacgatg gatactattc tcatcgaaaa tctcattaaa
      481 gttggtatgg ctgtcgttgt gggcggactc atcggtgcag aacgcgaata tcaggataag
      541 gccgcaggct ttcgcacgat cattctgata actgtcggtt caactttatt caccatcttc
      601 tcggtcagca tggatccggg ttttacacgc acgcgcgtgg ctgcgaatat cgtaaccgga
      661 atcggcttcc tcggcgccgg cgcaatcgtg cgtgaacacg ggaaaattgg tggacttaca
      721 accgccgcaa cgatctggct ggcagctgcc cttggcatgg gcatcggcgc cggagagtta
      781 atgttcgttt ccctttccac tgtgatcgtt gtcatcatcc tgctcgtttt cccgcgcatc
      841 gagagccgca tcgaccgcat tcgtgagtct cgtacctata aaatcgtagt tacttcacaa
      901 aacctggaaa tgaccgagaa tatctatgac tcgattcgag attgctcgtt acaggtgttg
      961 gagcatcacc aatcaaaaac tggcgagacc atcatcggca cctggcgcgc cattggttcg
     1021 ccggagaatc atgaaaagtt tgtcggtaaa atgatgagag ataagggcgt gaaggagcta
     1081 gtttactaaa ccgtcgacct aacaaaaatc aattacgtag agacgaccgt taatacaaaa
     1141 ggctctcata tggagagcct tttgtattat tttggttcta tatttccgct cgcgtctgtt
     1201 gatgcttcat tctccggctt atctttttca agtccatcac gaaaggcctt gattcctttt
     1261 ccgagctcag aagcaagttt gcctatgcga cccggcccaa aaagaagcaa tacgatcacc
     1321 agaatgacga tcaattccgc gccacgtggt agtccaggca tgtgacactc ctttactaat
     1381 tttcattcct atcctaccac aatcgatgcc ccttgaccac accctacaac tcgcggcgat
     1441 acaacaaacc ttcgatgaag cgtaaggcgc ggcgcacaaa tgagcgtaaa cgcccaaagg
     1501 tacgatactg ttccgggcga acatgtcctt tgcttggaat agggccggtc cattctgcag
     1561 cgagggcgcc ccaggtcaga ccggcgccga agcccaccat caccagctta tccccttgct
     1621 tgatctgacc tttttccagc gcttcgacca ttgcaattgg aatcgaagca gtggatgtat
     1681 taccatagcg ttcgagattc acgatgaagc gatccatcgg taacttgagc ccgcgcgccg
     1741 ccgcctcaat gatacggatg ttggcctgat gtggaatgat ccagcgaatc tcatcgatag
     1801 tcaagcctgc agcatggaga gcttcctgcg tcgccgaagc catgacgcgc gtcgcaaagc
     1861 gaaagacttc cttcccgtcc atttgaataa agtgccttcc agtgtggatg gttgcctcgg
     1921 aggctggata ccggcttcct cccccttcga tcgtgagcaa atcacctcct gagccatctg
     1981 agtgcatgac cgctgaaagc accccgcctg gctggtcgct ggcttgcagt acaaacgctc
     2041 ctgccccatc tccaaatagg atacaggtat ttcggtcttt ccagtttatg aatcgcgaaa
     2101 gggtttcaga gccaacaata agcgcgctct tgatcgagcc actacgaata gcctgtgcgc
     2161 ccatgttcaa agcgtaaata aagcctgaac aagccgccag caaatcgaat gcccccgctt
     2221 tggatgcacc cagttgatcc tgaatcaaac aagccgttgc agggaagatg tattcaggcg
     2281 atgaggtcgt acagatgatc aggtccagat cagtgggacg aagatttgca acagtgagtg
     2341 ccttgatcga agcctctacc ccaagcgtag aaggaaattc gttctctcgt gcaatacggc
     2401 gctcacgaat cccggtgcgc tcacgaatcc atgcgtcatt tgtctccacc agcttcgata
     2461 aatcatcatt tgtcataatc gactcaggcg ccgccatccc ccagcctgta atatgtgcgt
     2521 atggcataac atgctcctag atcatcattg attcaaacta tcaataaatc tatgatgatc
     2581 ttattcttca aagcgactga atatcaacgt tgcgttatga ccgccgaagc caaacgagtt
     2641 ggacatgaca tgacgtggtg aaaccttacg cggctcattc ggaacataat ccaggtcaca
     2701 ttgcgggtca ggtgtctgat agttaatggt cgccggcaag atcccatctc gaatcgccat
     2761 aatgctgaag acggcttcga ccgcaccaga ggcgcctaat aaatggccag tcatcgattt
     2821 tgtcgaagac acgggaatgt tataagcctg ctcgccaaac acagtcttaa tggcggctgt
     2881 ttcactctta tcgttcaaag gcgtagaggt accatgcgca ttgatatagc caatatcatt
     2941 tacagttaac tccgcatttt ccaaagctaa cttcatcgac atggctgcgc ccgccccatt
     3001 ttcggcagga gcagaaatgt gatgcgcgtc gtctgtggtc ccgtagccgc tcagttcacc
     3061 taaaatattg gcaccgcgcg cggtagcatg ctcaagggat tccaggatta gaattcccgc
     3121 cccctcaccc atcaaaaagc catcgcgatc cttatcaaac ggacgggacg ctgttttcgg
     3181 atcgtcattg cgggtagaaa gtgcgcccat gacattcatt cctgccatcg ccagcgacac
     3241 gagagaggct tctgaaccac ccgcgatcat tgcatcagcc gagccacgcc gaatcgtctc
     3301 aagagcatca ccaagcgcat tattgccaga tgcgcaggct gttgcaattg ccatattggg
     3361 tccacgcgca ccgacacgga tagccagcat gccaggtgca ctgtcagaaa tcatcatggg
     3421 aatcaagaaa ggactcacgc gctccggacc gcgttcgcgc attacctctg cctgatcgag
     3481 catagtgcca atcccaccga tgcctgttcc aatgacgatc ccgactcgat cccggttgga
     3541 ttcgtcaatt ttcagacctg attgttcgag tgcctgtcca gtggcagcga ctgcaaactg
     3601 cgtaaaacga tccattttcc gtgcttcacg gttcccaaac aaggcgacac catcaaagcc
     3661 tttcacttct gccgcaaatc ttgttttgtg cttgcttgca tcaaacaatg taatcatgtc
     3721 agagccagat ttacccgcca atatcgctga ccaagtctct gcaacattat ttcccacagg
     3781 gctaatacac cccatccctg tgattactac tcgtcttttc ataaacaaat ctcctgttac
     3841 gtttggctca caaattaatg taatttggcg ttaaattctt tttaattcag cccggcacat
     3901 tggaattgca caattttctt cgtaaaatac cggcaaaacc atctctgccc accaggttcc
     3961 actaggctgt tataacacac ccctctggaa ttcgacgcgc cagtgttcga gaccgttaca
     4021 agctattcag ggtcttccag gcttgttgaa tatgcattct gtcatgatcg gcaatgaagc
     4081 taattacctc aaggaaattg gtaggtccaa aaatggcgtg ccgcgcgcgg cgtgaccata
     4141 gctcactgtc ggaaccagcc agtttaagca aattggccaa ccgcgcggcg gtaaagtctg
     4201 ccagcgctgt aaggccatcc tcattcaaat actcgcgttc atttgcccac acgctagtat
     4261 cggggcgcgg aatgaatgca cccgaccgcg caagcatcag atccaattgc aagcggtgaa
     4321 tttccatttc agtatctcgc agatggcaga caatttcatt aacagcccaa tcttcgcgag
     4381 ttggctcgtg ccgccactct tcgtcat
//
LOCUS       DASTJP010000095         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_969839, whole genome shotgun sequence.
ACCESSION   DASTJP010000095 DASTJP010000000
VERSION     DASTJP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969839"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFQ23_02930"
     CDS             complement(<1..658)
                     /locus_tag="VFQ23_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET9905560.1"
                     /translation="MNRHLPPLPVRIVIVLIVLSTIGYFFFRSLSPVENGQLKASGTI
                     ESVTVNISPEMAGKVKEVLVSEGQPVKTGDPLLHLDDSLLTAQRALASAQLDSANSAV
                     TTAEAALAASQVQYNLAFDTAVIQSDTVRTVDWFRDEPSTFDLPLWYYSQSEQLTAAQ
                     SEVDLAKTALEAAQSNLISVLSSAGSADFVKAETDLAAARASYQVAKDIYDRIQNGLT
                     T"
     gene            complement(700..1824)
                     /locus_tag="VFQ23_02935"
     CDS             complement(700..1824)
                     /locus_tag="VFQ23_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905561.1"
                     /translation="MNSRLLSIVRKEFIQIFRDPRTLALVIVMPIMQLFLLGYAATTD
                     VRNIDMAVWDQNNTPQSRELLDAFRAADYFRIEYAVSSPDEYQALIESGKVRVALIIP
                     PDYDRRLLESTAQVEIILDGSDGSVGATALSTARLVGQSYATKIVAERTLLSGRTTSQ
                     VPLEVRTRVLYNPDFRSAYFMIPGVAGMILSFITTILTATAIVRERERGTIEQLIVTP
                     IRPIELVIGKLTPYVILAFIETFEVLIVGRLVFDVPIRGSLWLIILSSGLFLMSSLGI
                     GLFASTVANTQQEAMLTVMMFNLPMIFLSGFFFPIQAMPPALQIVSAVIPLRYYLIVI
                     RSLLLKGVGFAALQTEIISLAIFGLVIMTLASLRFRKRLD"
     gene            complement(1821..2429)
                     /locus_tag="VFQ23_02940"
     CDS             complement(1821..2429)
                     /locus_tag="VFQ23_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9905562.1"
                     /translation="MKKEKPTKGEVTRSAIEEAAIVLFMEQGYHATSVRQIAERANLA
                     LGGIYNHFSSKEEIFKGIIIDKHPYRVILPAIIAAEGETLEEFFKNAFHIIISELGGH
                     PEFVNLMLIELIEFKGKHGSMMLKEVAPKILPIFERVIKSRNDLRVTNPAVLMRSFIG
                     MVVSYLITEMVIANSVISKLLPKNSKDAYVDIYLHGILKEPA"
     gene            2599..3162
                     /locus_tag="VFQ23_02945"
     CDS             2599..3162
                     /locus_tag="VFQ23_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET9905563.1"
                     /translation="MAELAWIEIIYWASTIIGGTLFILRLIMMFVGGDFSDDGLDSAL
                     EVGDHAAADISFKLLSVQGLTSFFMMFGLVGLALLKANQPVFLTVVGGMLAGLVTVAV
                     SGLIFSQMKRLQTEGTINIQNTIGTEGVVYLTIPKNGTGQVQIIVQGSLKIFDAVSSQ
                     KNAIATGEKVRVSGISSGNTLIVDKSK"
     gene            3175..4791
                     /locus_tag="VFQ23_02950"
     CDS             3175..4791
                     /locus_tag="VFQ23_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9905564.1"
                     /translation="MMNAAGFGVLALIAFVVLAFLTVAFMASRYKRCPSDKILVIYGR
                     VEKGRSSKTMHGGGAFVWPLIQDFAYISLTPITISIPLENALSLQNIRINVPSTFTVG
                     VSTAAEIMSNAAERILHLQQGAIEEMAKEIIFGQLRLTVASLTIEQINQDRESFLDSI
                     RRNVEPELNKIGLQLINVNITDITDASEYISSIGKKAASEAINQAKIDVAVQSRHGAI
                     GEANAVREQEIKVAEAMAEAEIGRKTADANQRMRVQEQEASAVQGENTANANIARYNA
                     DLEIQRAKASADSEKGKKTAEADQRIFIQEQEAQAVRGENTSKANIADYNAELEVKRA
                     SALKHAEVARYEAQVAIQQALYKAEQERLRAEEVVRQEIEKNKIEIAAEAEAEKIRRE
                     AQGTADGILAKYKAEAEGTLKLLEAKADGYRKLVESVGGDAQAASTLLMIEKLEELVA
                     RQVEAISNLKIDKITVWDSGGNGDGSSTSNFISNLFKSLPPLQDISEMAGVQLPEYLG
                     KVSGQSTKALSDHNESGETRDSSPTKKSKS"
BASE COUNT         1357 a         1104 c         1260 g         1139 t
ORIGIN      
        1 cggtagtaag accattctgg atcctgtcat aaatatcctt agccacctga taactcgctc
       61 gtgcagccgc caggtctgtt tctgctttca cgaaatctgc actgcccgcg ctgctgagaa
      121 cagaaatcaa gttgctttgc gcagcttcca acgcagtttt tgccaaatca acttcggatt
      181 gggccgcagt gagttgctca gattgactgt aataccataa tgggaggtca aaggtactgg
      241 gttcatccct gaaccaatcc acagttcgaa cggtatccga ttgaatgacg gcagtatcaa
      301 acgccaggtt atattgcacc tgtgaagctg caagagccgc ttctgcagtc gtcaccgcgg
      361 aattggcaga atccaattga gcggaggcaa gcgcgcgttg agcggtcagc aagctatcgt
      421 ccaggtgaag aagtggatca cctgtcttca ccggttgacc ttccgacacg agcacttctt
      481 tcactttgcc tgccatctct ggtgagatat tcacagttac cgattcaatg gtgcccgaag
      541 ctttgagttg tccgttttca acagggctca aagagcgaaa gaagaagtat ccaattgttg
      601 agaggacaat caggacgatg acgatacgga cagggagagg gggtaggtga cgattcatct
      661 ttactcctta attattttca taagcgacaa atcaatttgt taatccaatc gctttctaaa
      721 acgcagcgag gcgagtgtca tgatgaccag accaaagatc gccaacgata tgatttccgt
      781 ttggagcgca gcaaatccga cgcctttcag taatagcgat cgaatcacaa tgagatagta
      841 gcgtagtggg atgaccgccg atacgatctg tagagccggg ggcatggctt gaatggggaa
      901 gaaaaatccc gagaggaaaa tcatgggaag gttgaacatc atcacggtga gcatggcttc
      961 ctgctgggta ttggccaccg tggaggcgaa caggccaatc cccaggctcg acatgaggaa
     1021 caaacctgat gaaaggatga tgagccagag gcttcctcga attggcacat cgaagactaa
     1081 acgaccaacg atgagaacct caaacgtctc gatgaaagcg aggatgacat acggggtaag
     1141 tttgccgatg accagttcaa tgggacgaat cggcgtaacg atgagttgct cgatggtgcc
     1201 gcgttctcgt tcgcggacaa tggcggtggc agtgagaatg gtggtgatga acgagagtat
     1261 cataccggcc acgccaggaa tcatgaagta agcggagcgg aagtctgggt tgtacaagac
     1321 acgtgtgcgg acctcgagtg gcacctgact ggtggttcgc ccgctgagga gtgtacgctc
     1381 cgcgacaatt ttggtagcgt aggattgacc caccagccga gcagtggaga gcgccgttgc
     1441 cccaacactt ccatcggaac catccaggat gatctccacc tgggcagtgg attcaagcaa
     1501 acgacggtca taatcgggcg gaatgataag cgctacacgc actttgcctg attcgatcag
     1561 cgcctgatat tcatccgggg agctgacggc gtattcaatg cgaaagtaat cggcggcgcg
     1621 gaacgcatcc aatagttcgc gtgactgggg cgtattgttc tgatcccaaa ccgccatatc
     1681 gatattgcgc acgtctgtcg tggctgcata gcctaagaga aataactgca taatcggcat
     1741 gacaatcacc agggccagtg tgcgcgggtc gcgaaagatt tgaatgaatt ccttacggac
     1801 gatggagagg agtcgagagt tcatgctggc tcctttagaa tgccatgcag gtaaatatcc
     1861 acataagcat ctttgctgtt ttttggtaac agtttgctga tcacggagtt tgcgatgacc
     1921 atttcagtaa tcaggtagga tacgaccatc ccgataaaag aacgcatcaa taccgctggg
     1981 ttggtcactc gcaaatcgtt tctagacttt attacccgtt caaaaatagg taatatcttt
     2041 ggagcaacct ctttcagcat cattgagccg tgtttccctt tgaattcgat gagctcaata
     2101 agcatcagat ttacaaattc cggatgacca cccagttcag aaataatgat atgaaacgca
     2161 ttcttgaaaa attcttccag cgtctcccct tccgcagcaa tgatagctgg caggatgacg
     2221 cgatatggat gcttatcaat gatgatgccc ttaaagatct cttccttgct tgaaaaatgg
     2281 ttatagattc cacccagcgc cagatttgcg cgttcagcga tctggcgcac agaggtggca
     2341 tgatagcctt gttccataaa caagacgatc gctgcttctt caattgctga ccgcgtgact
     2401 tccccttttg tgggtttttc cttcttcatg gcatctccat aaaaatgaac gaacgttcgt
     2461 ttttatttta tccaaagcgc ttgatttgtc aagaggattt ggtcaatgcc ggtaaaacat
     2521 tcgaagttgc aacgtttcca acaaaaatcc gtatatttac tcaaggtata attgactgga
     2581 caacagaaag ggagatcaat ggcagagctg gcctggatcg aaattatcta ttgggcatca
     2641 actattatcg gcgggacgct ctttattctt cgtctgatca tgatgtttgt tggcggggat
     2701 ttcagtgatg atggtctcga ctcagccctc gaagtgggag atcatgctgc tgcagacatc
     2761 agcttcaagc tgttatctgt acaagggtta acttcttttt ttatgatgtt tgggcttgtt
     2821 ggcctggctt tattgaaagc aaatcagccc gtctttttga cagtggtcgg gggaatgctg
     2881 gcaggtctgg ttactgttgc agtatctggt cttatatttt cacaaatgaa gcgcttacaa
     2941 acagaaggaa ccatcaacat tcaaaacacg attggaacag aaggggttgt ttatctgacc
     3001 attcctaaaa atggcacggg acaagtacaa attattgtgc aaggatcatt aaaaatcttt
     3061 gatgcagttt caagccagaa aaatgccatc gctaccggcg agaaagtccg agtgagtgga
     3121 atatcgagtg gtaatacgct gatcgttgat aaatcgaagt agttcaagga gaacatgatg
     3181 aatgctgctg gttttggagt tttagccctt attgcctttg tagttcttgc cttccttact
     3241 gtcgccttca tggcaagtcg gtacaaacgc tgtccgtcag acaaaatcct tgtcatttac
     3301 gggcgtgtgg aaaagggacg atcaagcaaa acgatgcatg gaggtggtgc ttttgtatgg
     3361 cctctgattc aagacttcgc ctacatcagc ctcaccccca ttacgatcag cattcctttg
     3421 gaaaacgccc tgtcattaca gaacatccgc atcaatgtgc caagtacatt taccgtcggc
     3481 gtcagcaccg cagcagagat tatgagcaac gcggcggaaa ggatcctgca cctccaacaa
     3541 ggtgccattg aagaaatggc aaaggagatt atcttcggtc aactgcgttt gacggttgca
     3601 tctctaacca ttgaacaaat aaaccaggat cgtgagagct tcttagattc cattcgccgc
     3661 aatgtggagc ctgaactcaa caagatcggc ctgcaactca tcaatgtcaa catcacagat
     3721 atcacagacg catccgaata tatctccagt attggcaaga aggccgcatc tgaggcgatc
     3781 aaccaggcga agattgatgt agcagttcag tccaggcatg gcgctattgg tgaggcgaat
     3841 gctgtgcgtg agcaggaaat caaagttgcc gaagcgatgg cagaagctga gatcggacgt
     3901 aaaacggcgg atgcaaacca gcgtatgcga gtgcaggaac aggaagcttc agccgtacag
     3961 ggtgaaaaca cagctaatgc gaacatagcc agatacaacg cagaccttga aatccaacgc
     4021 gccaaggcaa gtgccgattc tgaaaaaggg aagaagactg ccgaagccga tcaacgcatc
     4081 ttcattcagg aacaggaagc gcaggcagtc aggggagaga atacatcgaa ggccaatatt
     4141 gcggattaca acgcggaatt ggaagtgaaa cgtgcatccg cattgaagca cgctgaggtc
     4201 gcgcgctatg aagcgcaggt tgccatccag caggcgctgt acaaggcaga acaggaacga
     4261 ctgcgcgccg aagaagtggt tcgtcaggag atcgaaaaga acaagataga gatcgctgct
     4321 gaggctgagg cagagaaaat ccgccgcgag gcgcaaggga cagcggatgg cattctggca
     4381 aaatacaagg cggaggcaga aggtacactc aaactgctgg aagcaaaagc ggatgggtat
     4441 cgtaaactgg tcgagagtgt tggaggcgac gcacaagccg catccacatt gttgatgatc
     4501 gaaaagctgg aggagcttgt cgcccgacag gtagaagcca tcagcaatct taagatcgac
     4561 aagatcacgg tttgggactc gggtggcaac ggcgacggtt cgtctacatc caacttcatt
     4621 tcaaacctct tcaagagcct gccaccactt caggacattt ctgaaatggc tggcgtgcag
     4681 ttgccggaat atcttggcaa agtaagtgga caaagcacga aggcactaag cgatcataac
     4741 gaatcaggcg aaacacggga ttcttccccc acaaaaaaaa gtaagtcata aatctaacag
     4801 gaataaaagc gtttcaaagc ccagttgaac actgggcttt ttcatggtca gagaagataa
//
LOCUS       DASTJP010000096         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1287864, whole genome shotgun sequence.
ACCESSION   DASTJP010000096 DASTJP010000000
VERSION     DASTJP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287864"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFQ23_02955"
     CDS             <1..615
                     /locus_tag="VFQ23_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9905565.1"
                     /translation="YTENLKASKDGTLRFNERSGLVDDSVLNENLRKVYGDWQFSDLK
                     LPFKVVATDLITAEKVILSEGSLVDAIRASVAIPIIFPPWEVNGRLLIDGAASDPLPI
                     DVAIQDGADVIIAMGFTLDYRARFRSMTAVQEQLNSIYMNNILNATYAFYNLAHHAEI
                     FPVIPEFDSTISMFDVDKIPEVIERGAQAARKQLPHIQKVLASA"
     gene            complement(654..729)
                     /locus_tag="VFQ23_02960"
     tRNA            complement(654..729)
                     /locus_tag="VFQ23_02960"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(694..696),aa:Ala,seq:ggc)
     gene            complement(802..874)
                     /locus_tag="VFQ23_02965"
     tRNA            complement(802..874)
                     /locus_tag="VFQ23_02965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(839..841),aa:Ala,seq:cgc)
     gene            1187..1894
                     /gene="radC"
                     /locus_tag="VFQ23_02970"
     CDS             1187..1894
                     /gene="radC"
                     /locus_tag="VFQ23_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET9905566.1"
                     /translation="MNETISKPVYRIMDLHESDRPRERLASLGPQALTNAELIAILLR
                     VGVKGESAVAVGQRLLNNFGALTGLHRAPFADLKKQHGLGDAKAAQIKAAIELGRRLT
                     LESPEERPTINSPADAAALVSYEMSALEQEHLRVLLLDRRNRVMETVEVYKGSVNSSQ
                     VRIGEVFKEAVRKNASALIVVHNHPSGDPTPSPDDVAVTRAIVQAGKLLDVEVLDHLV
                     IGQGKWVSLKERGLGFA"
     gene            2016..4661
                     /locus_tag="VFQ23_02975"
     CDS             2016..4661
                     /locus_tag="VFQ23_02975"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9905567.1"
                     /translation="MAAPILATKLYIPPPRPKVVTRTRLIERLNEGLHHKLTLISAPA
                     GFGKTTLVSEWVSNCGRSVSWLSLDKGENDPARFLTYFISSLQIISSNFGEKVLGLIQ
                     ASQPQPPSLESILTPLLNEITAIPQDFILVLDDYHVIDSKHIDSGLAFLIEHLPPQMH
                     LVIVTREDPDLPLARLRGRDQLTELRAADLRFTSSEAAEFLNQVMGLNLSAQDIGALE
                     TRTEGWIAGLQLAAISMQGQKDVSNFIKSFTGSHHFVMDYLVEEVLKRQPEDVQNFLL
                     RTSVLNRLCGSLCDAVLLDASASSEETLEYLERANLFLIPLDNERRWHRYHHLFSDLL
                     RQRLNQSVAASVVKELHIRASQWYEDQNLVMEAFHHAAAANDVERAERLIEGKGVPLH
                     FSGGVVPILEWLDSLPDETLNSRPSLWWRHAALLLINGQTIGVGEKLQAAELALSATL
                     QGTEPDEKTRNLIGQIASARATLALTRYDVQGMLTQSSRALEYLSPGALFNRATANWV
                     LGFAQFLLGNRLAARQAFTEGIALSQKSAAMFPMILATIGLGNIQEADNELYQAAETY
                     RRVLEWAGEHPQQIIHEAHLGLARVLYEWNDLEGAEQHGQHSLVLARQYEGVIDRYII
                     CEVFLARLKLARGDVDGAAAALAQTNQTARQQNFVHRIPEVAAAQILTLIRQGNLSVA
                     ANLAETHKLPLSLARVHLARGDTDAALKILETWGTQAEAKDWKDERLRVKILQSLAHQ
                     ANGEMDVALQRLGEALTLAEPSGFIRLFVDEGTPMAEILSEAAARDVMPAYVRKLLDA
                     FAAEPGREVNSKPPPSEALIEPLSQRELEVLQLIAKGLSNQEISERLFLALDTVKGHN
                     RRIYDKLQVQRRTEAIARARELDLL"
     gene            4791..>5199
                     /locus_tag="VFQ23_02980"
     CDS             4791..>5199
                     /locus_tag="VFQ23_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9905568.1"
                     /translation="MDIYEALYTTRAMRRLKPDPIPYDVQARILDAAIRAPHIGEGWR
                     FILLDDPQIKAQFAPLYRQGFERMIGGPPEEIFADVLKTDTPMAKTVRSGLYLVNHFA
                     QVPLFLIGFGRTKDGAGIYPALQNAMLAARAEGI"
BASE COUNT         1278 a         1383 c         1360 g         1178 t
ORIGIN      
        1 tataccgaga acctgaaagc cagcaaagac ggtaccctgc gcttcaacga acgaagcggg
       61 ttggtggatg attcggtgct gaacgaaaac ctgcgcaaag tgtatggtga ttggcaattt
      121 tcggatctca aactgccgtt caaagttgta gcaaccgacc tgatcactgc agagaaggtg
      181 atactttccg aaggcagcct ggtagacgcc atccgcgcca gtgttgccat ccccatcatt
      241 ttcccgccct gggaggtgaa cggacgattg ctgatcgatg gagctgcctc agacccgctg
      301 cctatcgatg tcgccattca ggatggcgcg gatgtgatta tcgcgatggg cttcacgctt
      361 gattatcgtg cacgcttccg gtcgatgact gccgtgcagg aacaattgaa cagcatttac
      421 atgaacaaca ttctgaacgc cacctacgcg ttttataacc tggctcacca tgctgagatc
      481 tttcccgtta tccctgagtt cgatagcacc atctccatgt tcgatgtgga taagattccc
      541 gaagtgatcg agcgcggcgc acaggccgca cgtaaacagt tgccgcatat ccaaaaagta
      601 ttggcaagcg cttaaacaaa aaaaccgccc tctgaacggg gcggttttct tactggtgga
      661 cccaggggga ttcgaacccc cgaccttctc aatgccattg agacgcgctc ccaactgcgc
      721 tatgggcccg gatttcagtg atcagctttt accgcattct gcggcactat gtaaatgttc
      781 agcaaccaat cactgaacag gtggacctga ggggattcga acccctgacc tcctcagtgc
      841 gattgaggcg cgctcccaac tgcgctacag gcccataaat gattatcccg caaatgcggg
      901 gcaaggcgaa tgacattcta cttgaggcat atggctgtgt caagcaacat attccagggg
      961 agtggctaag agagtgagtg aaactctact tagccacaga gagcactgag ccctttgaga
     1021 aaaaaactca aaaactctgc gggctctgtg gcttacacac taaattcgcc actccctatt
     1081 ccacgatcca ggttttcttg caatgcgaag atcgttaaga ctttgaaaag attcacacat
     1141 ccacttcctt gtaaaatagg cagagatctt tcctgaggga aactttatga acgaaacgat
     1201 atccaaacct gtctatcgca tcatggactt gcacgaatcg gatcgcccgc gcgagcggct
     1261 ggcttcgctg ggtcctcagg cgctaactaa tgcggagttg atcgccattc tcctgcgcgt
     1321 gggcgtgaaa ggcgaaagcg ctgtcgcggt cggtcagcgc ctgctcaata attttggtgc
     1381 gctgacgggt cttcaccgtg cgccatttgc ggatttgaaa aagcagcatg ggctgggcga
     1441 cgccaaagcc gcgcagatca aagccgcgat cgagctgggt cggcggctga cgctcgaatc
     1501 cccggaagaa cgtccaacga tcaacagtcc agcagatgca gccgcgctgg tttcgtacga
     1561 aatgtctgcc ctggagcagg aacatctgcg cgttctttta ttagatcgaa ggaatcgcgt
     1621 gatggaaacg gtggaagtct ataaaggctc tgtcaactct tctcaggtga gaattggcga
     1681 agtgttcaaa gaagccgtac ggaagaatgc atcggcattg atcgtggtcc acaatcaccc
     1741 cagcggtgac ccgaccccca gcccggatga tgtggctgtg acgcgcgcca tcgtacaggc
     1801 cggtaaatta ctggacgtgg aagtcctgga tcatttggtg attggtcagg gcaagtgggt
     1861 gtcgttgaag gaaagaggct tggggtttgc gtaagaattc ataagatgtc agatgcctac
     1921 agcatctaat tgaacgtaga aacaaatcaa agaaaacgtc ccaacataag ttgggacgtt
     1981 tttgaatcgg gggatgtgtg ttttataatt gttttatggc tgcaccgata ttagccacaa
     2041 aactgtatat ccctccgcct cgaccaaagg tagtcacgcg cactcgcctg atcgagcggc
     2101 tgaatgaggg gctacaccac aagctgacac tcatctctgc gcccgcaggt tttggcaaaa
     2161 ctacgctcgt cagcgaatgg gtttccaact gcgggcgatc ggtttcatgg ctctctctcg
     2221 acaaagggga gaacgacccc gcacgttttc ttacttactt catctcctcg ttgcaaatca
     2281 tttcatcaaa ttttggagaa aaggtattag gcctgatcca ggcttcgcag cctcagccac
     2341 catcccttga atctattctg actcctctgc tcaatgaaat cacagcaatc ccgcaagatt
     2401 tcatcctggt tctggatgat taccatgtaa tcgattccaa acatatcgac agtggccttg
     2461 ccttcttaat cgagcacctg ccgcctcaga tgcacctcgt catcgtcact cgcgaggacc
     2521 ccgatctacc gctcgcgcgg ttacgtggtc gtgaccaatt gactgaactg cgcgcggcag
     2581 acttgcgttt cacttcctcc gaagctgccg aatttctcaa tcaggtcatg gggcttaacc
     2641 tctccgcaca ggatattggc gcgctggaaa cgcgaaccga aggctggatc gccggcttgc
     2701 aattagctgc catttcaatg caaggacaaa aagacgtcag caatttcata aaatcgttca
     2761 ccggcagtca tcatttcgtg atggattatc ttgtggaaga ggtcctgaaa cgacaacccg
     2821 aagacgttca aaacttccta ttgcgcacat ccgttctcaa ccgattatgt ggctccttat
     2881 gtgatgcagt tctgctagat gcgtcggcat ccagcgaaga aacgttggaa tatcttgaac
     2941 gcgccaacct tttcctaatt ccgctggata atgaacggcg atggcatcgc tatcaccacc
     3001 tcttttccga tttgctgcga cagcgactta accagagtgt cgctgcatcc gtggtaaaag
     3061 aactgcacat tcgagccagc cagtggtacg aagatcaaaa cctcgtgatg gaagcatttc
     3121 atcatgctgc agctgccaat gacgtcgagc gcgctgagcg gctcattgag ggaaaagggg
     3181 ttcctctaca ttttagtggc ggggtggttc cgatactgga atggctggat tcgttacccg
     3241 atgaaactct gaattcaaga ccttccttgt ggtggcggca cgccgccttg ttgctgatca
     3301 acgggcagac gattggtgtc ggtgaaaaat tacaagccgc agaattggct ttatctgcaa
     3361 cgcttcaagg cactgaacca gacgaaaaga ctcgaaacct cattgggcaa atcgcatccg
     3421 ccagagctac cctggcactg acacgctacg atgtgcaagg catgcttact caatccagcc
     3481 gtgccttgga atatttgagt ccgggtgctc tctttaatcg cgcgacggcc aactgggtac
     3541 ttggatttgc tcagttcctc ctgggaaatc gtctcgcggc tcgccaggcc ttcacggaag
     3601 gcatcgctct cagccaaaaa tccgcggcca tgttcccaat gatattggcc accattgggt
     3661 tgggaaacat ccaggaagcg gataacgagc tgtatcaagc cgctgaaacc tatcgacgtg
     3721 ttctggaatg ggcgggtgag catccgcagc agatcatcca tgaagcacat cttgggctag
     3781 cccgcgttct ttacgaatgg aacgatttgg aaggcgctga acagcatgga caacacagcc
     3841 tggtactggc gcggcaatac gaaggagtga tcgaccgtta catcatttgc gaggtgttcc
     3901 tcgctcgctt gaaactggcg cggggcgatg tggacggcgc cgcggctgca ctcgctcaaa
     3961 cgaaccagac ggcgcggcaa caaaactttg tgcatcgcat ccccgaagtt gcagctgcgc
     4021 agatcttgac cctgattcga cagggcaacc tatcagtggc tgcgaatctg gctgagactc
     4081 acaaactccc tctaagcctg gcacgagtac acctcgctcg gggcgataca gatgccgcat
     4141 tgaagatttt ggaaacatgg ggcacacaag ccgaagccaa agattggaag gatgagcggt
     4201 taagagtgaa gatactgcag tcccttgcac atcaggccaa tggcgaaatg gacgttgcac
     4261 ttcagcgact gggagaggct ctcactctgg cggaacccag cgggttcatc cgtctctttg
     4321 tcgatgaagg aaccccaatg gctgaaatat tatccgaagc ggctgctcga gatgtgatgc
     4381 ccgcctatgt gagaaagctg ctggatgcat ttgcagcaga gcccgggcgc gaggtcaatt
     4441 ccaaaccgcc tccttctgag gctctgatcg aacccctgag ccaacgcgaa ctggaagtgc
     4501 tgcagctcat cgcaaaagga ctctcgaatc aggagatcag cgagcggctc ttcctggctc
     4561 ttgacaccgt caaagggcac aaccgcagaa tttatgacaa acttcaggtc cagaggcgca
     4621 cggaagcgat cgcccgtgcc cgtgagttgg atttgcttta gctgaagatg atcttgaatg
     4681 ggggaactac aaagtttaac acccgaaaca acactttcgt gtctatatcg caaaagtaga
     4741 tgtggatata ttacctgtac atcaagcaag ttgtacaaag gaattgcatt atggacattt
     4801 acgaagcgtt gtatacgacc cgcgccatgc ggcgactgaa acctgacccg atcccctacg
     4861 atgtgcaggc tcgcattctg gatgcagcca ttcgcgcacc ccatatcggg gaagggtggc
     4921 gattcatcct gttagacgat ccgcagatca aggctcagtt cgcgccgctc tatcgacaag
     4981 gattcgagcg catgatcggc ggcccaccgg aagagatctt cgcggacgtt ttgaagacgg
     5041 atacgccaat ggccaaaacg gttcggtccg ggctgtacct ggtgaatcac tttgcccagg
     5101 tgcccctatt cctcatcgga ttcggtcgta ccaaagacgg tgcaggtatc tacccggccc
     5161 tccaaaacgc aatgctggct gcccgcgccg agggcatcg
//
LOCUS       DASTJP010000097         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_903412, whole genome shotgun sequence.
ACCESSION   DASTJP010000097 DASTJP010000000
VERSION     DASTJP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903412"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFQ23_02985"
     CDS             complement(<1..323)
                     /locus_tag="VFQ23_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET9905569.1"
                     /translation="MSTLWGGRFAQKLDSGAWDFNSSLAVDQRLALQDVQGSLAWADA
                     LHTANVLSDEEYSSISLGLATVKKEFTSGKFLFAPTDEDIHTAVERRLTELIGDAAGR
                     LHTGR"
     gene            complement(325..1596)
                     /locus_tag="VFQ23_02990"
     CDS             complement(325..1596)
                     /locus_tag="VFQ23_02990"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET9905570.1"
                     /translation="MSPKLKPQVKKVVLAYSGGLDTSVIVPWLKEHYGCEVVCFCADI
                     GQGDEDLYGLEQKAINSGASQCIIRDLRNEFAAEYLFPMLKMGAVYERKYLLGTSVAR
                     PLIAKHLVDVAHEVGADAIAHGATGKGNDQVRFELTVMALDPRLKIIAPWREWDIRSR
                     EDAIAYAAKHQIPVTATVKSIYSRDSNLWHLSHEGGLLEDPWNEPEEAMYQISTSPEN
                     APEQAEYVEIEFESGVPCAVNGEILPPEKIIETLNAIGGAHGIGRVDLVENRLVGMKS
                     HGVYETPGGTILLYAHRELESLVIDRETMHFKEMAAVKYAELTYNGLWFTPLREALDA
                     FVNATQGPVTGKVRLKLYKGNIIVAGRTSPFSLYREDFATFGQEDVYDQSHAEGFIRL
                     FGLSLKVRALNGLPVSGLDMPKPDYSRFKRD"
     gene            complement(2021..>2322)
                     /locus_tag="VFQ23_02995"
     CDS             complement(2021..>2322)
                     /locus_tag="VFQ23_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905571.1"
                     /translation="AAAVLEAEILSAAKHAGIPIQQTRVGTMSTTFFSETKPVDWNTV
                     KVADTVRFGKFFRTMLENRVYLAPSQFEAGFVSIVHDTTVIDETAAAARIAFRTC"
BASE COUNT          563 a          605 c          566 g          588 t
ORIGIN      
        1 cttcgacccg tgtggagccg gcctgccgcg tctccaatta attcggtaag gcggcgttcg
       61 accgcggtgt ggatatcttc atcggtaggc gcgaagagaa acttccctga ggtgaattct
      121 tttttgacgg tagcaagccc gagcgagatc gagctgtact cctcatccga aagaacgttt
      181 gccgtatgta aagcatccgc ccaggcaagg ctaccctgaa catcttgcag tgccaggcgt
      241 tggtcaacag ccagagatga gttgaaatcc caggcgccag agtcgagttt ttgagcgaag
      301 cgtccgcccc agagtgtgga catgctaatc tctcttgaag cgcgaataat cgggtttggg
      361 catatcgaga ccggacacgg gcagtccatt cagcgcacgg actttgaggc tcagcccaaa
      421 gaggcggata aacccttcgg catgactctg atcgtagacg tcctcctgac caaacgtcgc
      481 gaagtcttca cgataaaggc tgaacggcga tgtgcgacct gcgacgatga tattgccctt
      541 ataaagcttg agccgcactt tgcccgtcac cggaccctgg gtggcattga caaaggcatc
      601 cagcgcctcg cgcagtggcg tgaaccagag accgttgtac gtgagctcag catatttcac
      661 cgcggccatt tctttgaagt gcatcgtttc gcggtcgatg accagtgatt caagttcgcg
      721 atgggcatac agcaggatcg tcccgccggg ggtttcatac acaccgtgcg acttcatccc
      781 caccaggcgg ttctcgacga gatccacacg tccgattccg tgtgcgccgc cgatggcatt
      841 gagagtttca ataatcttct ccggcggcag gatttctccg ttcaccgcgc agggaacacc
      901 actttcaaat tcaatttcca cgtactctgc ttgttccggt gcgttctcgg gtgaggtgga
      961 aatctggtac atggcttctt caggttcatt ccaagggtct tcgagcaacc caccttcatg
     1021 ggaaaggtgc cagagattcg agtcgcggct gtaaatggat ttaacagtcg cagtgacggg
     1081 aatctgatgt ttggctgcgt atgcaatcgc atcttcgcgc gagcgaatgt cccattcacg
     1141 ccagggagcg atgattttaa gtcgcgggtc aagcgccatc actgtcagtt caaaacgaac
     1201 ctggtcattt cctttgccgg tcgcgccatg agcaatggca tctgcaccga cttcatgagc
     1261 tacatctaca aggtgtttgg cgattaaagg acgggccacg gaggtgccca ataaatactt
     1321 acgttcatac accgccccca tcttgagcat cggaaaaaga tattcggctg caaattcatt
     1381 tctgagatca cgaatgatgc attggctcgc cccactgttg atcgctttct gttcaagtcc
     1441 gtacaggtct tcgtcaccct gaccgatatc tgcacaaaag cagacgactt cacaaccata
     1501 atgttccttc aaccacggaa caatgacaga ggtatccaat ccgcctgaat aggcgaggac
     1561 aaccttctta acttgcggct tcaacttcgg actcatttat ttctcctttt gtcttttaga
     1621 ccgtaaaggt ctgctttgta atctactttt atttatttct tcttgggact ttacacaggc
     1681 aaagtctcaa tccgcaggaa agacctttag ggtctatata acgggaaaat aaaaacagcc
     1741 cgccgagaag gcgggctgtt tgtgtggact tatacgttag cgtcagcgct gtgttgcgtt
     1801 ttctgctatt ccgtcatcca aacgaaatgc tcccaacctt ctcagggaag tgggcttcgg
     1861 acgacgcgaa cgcggcaacg caaactgata actcatattg ggcggtattc taccattcag
     1921 aataaatatg tcaatgcttt tgatagtacc cgtttgggta actaacaata aaagagaatt
     1981 acccatttag ggtatggtgc taaccaatag aaataatcag tcagcatgtt cgaaacgcaa
     2041 tccttgccgc agcagcagtt tcatcaatta ccgtcgtatc atgtacgatc gaaacaaatc
     2101 ccgcttcgaa ttgcgacggc gcaagataaa ctcgattctc caacatcgtt ctgaagaatt
     2161 tcccaaagcg gactgtatcc gccactttga ctgtattcca atctacaggt tttgtttcac
     2221 taaagaaggt tgtggacatc gtaccgactc gcgtctgttg aattgggatt cctgcgtgtt
     2281 ttgctgcaga taaaatttct gcttccaaca cagccgcagc ct
//
LOCUS       DASTJP010000098         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1014266, whole genome shotgun sequence.
ACCESSION   DASTJP010000098 DASTJP010000000
VERSION     DASTJP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014266"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFQ23_03000"
     CDS             complement(<1..482)
                     /locus_tag="VFQ23_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9905572.1"
                     /translation="MGNYLVTGAAGFIGARTSELLIKDGHRVVGIDNMNDAYDPRMKE
                     YRLQKMKTLPGMIFHQLDISDKSIIEQFKGEKFDGIINLAARAGVRYSVENPWIFVES
                     NMIGTLNMLEICRHTGTKKFIIASTSSIYGGDPPYPTPEYASSSEPLQPYAASKKGAE
                     A"
     gene            complement(483..1805)
                     /locus_tag="VFQ23_03005"
     CDS             complement(483..1805)
                     /locus_tag="VFQ23_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET9905573.1"
                     /translation="MSIKETLITKLKEKNARMAILGLGYVGLPLAVVFGEAGFKVTGI
                     DPDRRKIDAIEQGMSYIPDVDSEAVDKLVKSGNLTATTDFSVLKDIDAVSICVPTPLR
                     NTGDPDMSYILSATEELAKYMHKGMVIVLESTTYPGTTRELLLPKLGTERGLTVGEDW
                     FLAFSPERVDPGREDWTTINTPKVMGGITESCGEVAAAWYEGAINTVHRVSSAEAAEM
                     AKLLENTFRMINIGLVNELAIMCERLGIDVWEVIDAAASKPFGFMKFSPGPGLGGHCI
                     PIDPLYLSWKMKSFNYNARFIELASEINTNMPRYVVNRVMEALNERGKALKGSKVLIL
                     GAAYKADVDDVRESPALDVIGLLRNKGALVDYHDPYIPHIHHENEGWHMDSVTDLMSS
                     VKDADIVVIITDHKAYDYADIVEAAQSVFDSRNATRKFVKNSEKVVRL"
     gene            complement(1919..2395)
                     /gene="rimI"
                     /locus_tag="VFQ23_03010"
     CDS             complement(1919..2395)
                     /gene="rimI"
                     /locus_tag="VFQ23_03010"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET9905574.1"
                     /translation="MTLLIRKMTLDDLDQVTAIDRVSFSLPWPERSFRFDLTDNPASR
                     CWVAELDGKVVGMIVSWLIVDEVHVATIATHPEYRRRGIAKRLLTHTLQHLKEEGAQS
                     SFLEVRASNFAAQDMYRKFGYEETGLRPRYYKDNDEDAILMTLESLDVEKLEDQEA"
     gene            complement(2392..>2823)
                     /locus_tag="VFQ23_03015"
     CDS             complement(2392..>2823)
                     /locus_tag="VFQ23_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905575.1"
                     /translation="KGLALSRQIPLISIPTLDILASAQPASKLPLAVLIQAGRGRYAL
                     GWYKSLKNRWQVTDPAKVITIQALLDEIKSPSIVCGEFTSEDRRLLEESQNVQLASPA
                     LSVRRPAVLGELAWERWQAGEVDDEATLAPIYLHTAEAISI"
BASE COUNT          682 a          757 c          631 g          753 t
ORIGIN      
        1 gcctctgccc cttttttgct tgccgcgtat ggctggagcg gctcgctgct cgaagcgtac
       61 tcaggcgtcg ggtagggagg atcgcctcca taaatgctcg aagtggaagc aatgataaat
      121 ttcttcgtcc cggtatgccg gcaaatttcc agcatattca gtgttccgat catgttactt
      181 tctacaaaga tccacgggtt ctcaacgcta tatcggacgc cagcccgagc agccaggttg
      241 ataatgccat caaacttttc acctttaaat tgctcgatga tggatttatc agaaatatcc
      301 agttgatgaa aaatcatgcc tggtaatgtc ttcatctttt gcaggcgata ctctttcatg
      361 cgcgggtcat aggcatcatt catattgtca atacccacca ccctatgacc gtctttgatg
      421 aggagctctg aggtgcgcgc accgataaac ccagctgcgc ctgtgactaa ataattaccc
      481 atttaaagcc tgaccacctt ttcactgttc tttacgaatt ttcgcgtcgc attgcggctg
      541 tcaaaaacgg attgcgccgc ttcgacaata tcggcataat catatgcttt atggtctgtg
      601 atgatgacca cgatatctgc gtccttaact gagctcatca ggtccgttac actatccatg
      661 tgccagcctt cgttttcgtg atggatatgg ggaatataag gatcatgata gtccacaagc
      721 gctcccttgt tgcggagcaa accgatcaca tccagggcgg gagattcgcg tacatcatcc
      781 acgtcagctt tgtaagccgc acctaatatc aaaacctttg aacctttgag cgctttgccg
      841 cgttcgttca acgcttccat gacacggttg accacatagc gcggcatatt cgtgttgatt
      901 tcagaagcaa gttcgataaa tcgcgcgttg taattgaagg acttcatctt ccaagaaaga
      961 tacaacgggt caattggaat acaatgccct ccgaggccgg gtccgggtga gaatttcata
     1021 aacccaaatg gtttggatgc cgccgcatcg atcacttccc aaacgtcaat gccaagtcgt
     1081 tcgcacatga tcgccaattc atttaccaga ccgatgttga tcatacggaa cgtattctcc
     1141 agcagcttgg ccatctctgc cgcttcggct gaactcacgc gatgaaccgt gttgatcgct
     1201 ccttcatacc aggcagccgc aacctcgccg caggattccg tgattccgcc cattactttg
     1261 ggtgtgttga tcgttgtcca atcttcacgg ccagggtcaa cgcgctccgg agaaaatgcg
     1321 aggaaccaat cttcacctac cgtaagcccg cgctctgtgc caagtttagg taacaacagc
     1381 tcgcgcgtcg tacccggata cgttgttgac tcaagcacaa tgaccatgcc cttatgcata
     1441 tattttgcca gttcttcggt cgcggacaaa atgtaggaca tgtccgggtc gcctgtatta
     1501 cggagaggcg ttggcacaca aatactgacc gcgtcgatat cttttaagac tgaaaaatct
     1561 gtcgtcgccg tgaggttccc tgatttaacc agcttatcga ctgcttctga atctacatca
     1621 ggaatataag acataccttg ttcgattgcg tcaattttgc gacgatccgg gtcaatgccg
     1681 gtgactttga atcccgcttc accgaagaca accgccaggg gtagacccac atagcccagc
     1741 cccagaattg ccatgcgggc attcttttct ttcagtttcg taatgagtgt ttctttgatg
     1801 gacatttctt tcccttgtag actctaaagg tttcttgagt actcaaactc gcaatttgcc
     1861 aactttttca acaaattttc tatcatttgc cagaaagcct ttggggctaa attttatatt
     1921 aagcctcttg atcttccagc ttttcaacat ccagcgattc cagagtcatc aggatggcat
     1981 cttcatcatt atctttataa tagcgcggcc gaagcccggt ctcttcgtat ccgaatttgc
     2041 gatacatatc ctgtgcggcg aagttacttg cacgcacctc caaaaatgaa ctctgcgcgc
     2101 cttcctcttt caaatgctgc aacgtgtgag tcagaagtct cttcgcaatg ccacgcctgc
     2161 ggtactcggg atgggtagca atggtcgcca cgtgaacttc atccacgatc aaccacgaga
     2221 caatcattcc aactactttt ccatcgagtt cagccaccca gcatcgagaa gcagggttat
     2281 ccgtcaggtc aaacctgaaa gatcgttcgg gccacggcaa actgaacgac acccggtcaa
     2341 tagcagtgac ctgatccaga tcatccagag tcattttgcg aatgagcagg gtcatatgga
     2401 tattgcctca gcggtatgta agtaaatagg ggcaagagtt gcttcatcat ctacttcgcc
     2461 tgcctgccag cgttcccatg ccagttcgcc tagcacagcc ggtcgccgca ctgacagtgc
     2521 cggagatgca agctgcacgt tttgactctc ctccaataaa cggcgatctt cggatgtaaa
     2581 ctcaccgcac acgatggatg gactcttaat ttcatccaac agtgcctgaa tggtaatcac
     2641 tttcgccgga tctgtgacct gccaccgatt cttcaaactt ttataccagc ccagagcata
     2701 tctgccgcgc cctgcttgaa tgaggaccgc caatggaagt tttgaggccg gttgtgctga
     2761 cgcaagaatg tccagcgttg ggatactgat caaaggaatc tggcgtgaca acgccagtcc
     2821 ttt
//
LOCUS       DASTJP010000099        11853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1090099, whole genome shotgun sequence.
ACCESSION   DASTJP010000099 DASTJP010000000
VERSION     DASTJP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11853
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090099"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFQ23_03020"
     CDS             complement(<1..973)
                     /locus_tag="VFQ23_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9905576.1"
                     /translation="MSQLDDSKVSREPDEAEQERVESDLRSRERYLSLLNEMTRAILL
                     SEDFDSTLSTLAVNLAKLIDADDCYITRWDEEQEVTIPTTTTAKLEIPYPTRRTPADQ
                     LTLTASVLKLGRALAADDVFNSPYLSAEVAKRYPARSALGVPLIAGDHKLGAAIIVFN
                     TPHSFTSEEIERADHAADHIALALWNFQQSTEIQHRLKESNALAEIGRALSETEHTGT
                     DQILQLIVDSARELISNAEESVIHLLEAEAQALWARAVSGYPEQGTEFKRVQMRLGEG
                     VAGQVIREGITINIGDVETDPRFLLSSSIPNFRSLLVAPVQSGGQQIG"
     gene            complement(998..2527)
                     /gene="rny"
                     /locus_tag="VFQ23_03025"
     CDS             complement(998..2527)
                     /gene="rny"
                     /locus_tag="VFQ23_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET9905577.1"
                     /translation="MNTLTIILIALFALGLGAAAGYFLHRYQADKALKNQQDRADNIL
                     KGASEQARLIESQARENAVKIVQAAESEIKERRIELSKETDRLDKRRSEFENRVEKLE
                     QREQAINKRQSQVDKRGNEIDKLYEDQLKKLEQVAQLTTEDARKELFAATEKEARGDM
                     ARIIRQIEAEAREEGEKRARKLIADAIQRVASEHVAEVTRAVVTLPNEEMKGRIVGRN
                     GRNIKAFEQAAGVDVIVDDTPDSVTVSCFDSVRREIGRRALAKLILDGRIHPAHIEKI
                     VEDETRAVEKIINEAGEQAAFDANITGLHPEVLRMMGRLKFRTSYGQNQLAHAVEVSK
                     LAGILAAEIGANVEVSKQGGFLHDIGKAMDHNQEGTHAKLGAEFCKRYGVNSIVVNAI
                     ESHHHEVDQLTVEAVIAEAADAISGARPGARREDLEAYIKRIRSLEEMSMSFEGVQQA
                     FAIQAGREVRIIVKPDAIDDLASARLARDVAKKIEETMQYPGQIRVTVIRETRSTEIA
                     K"
     gene            complement(2524..3177)
                     /locus_tag="VFQ23_03030"
     CDS             complement(2524..3177)
                     /locus_tag="VFQ23_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HET9905578.1"
                     /translation="MKKITALTAQKRNPNRVNIYIDGEYAFSLARIVAAWLKTGQELD
                     DEKINRLQSEDARERAVQQSLLFLSYRSRSEAEIRQNLRKHEIPDEIIEHTLQRLRQE
                     GLANDDQFAQAWVENRSTFRPRSRRMMAMELRQKGVTDESVSTALEAVDDEELAYEAA
                     QKKVNRLKDLEWMDFRKKLSEFLARRGFSYSVIAPIVTRIWNEVHTDEHHFEEEDTT"
     gene            complement(3655..4266)
                     /locus_tag="VFQ23_03035"
     CDS             complement(3655..4266)
                     /locus_tag="VFQ23_03035"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET9905579.1"
                     /translation="MSEFLLTQVINFGAPILGLILFIGAIGAPFPGTFLVVAVGAFCR
                     QGLLEWHITGLIALICVVLGDCLSYSMGYYAREPILQRFSASRQWAHGENSFRRWGGM
                     SVFLTRFLITGIAVPVNLIAGTGSFPFRRFLMYDVLGEAIWVFGYGGLGYLFGTQWEL
                     VSDFISNFGGFALGLVILCVGVWLGFQRLRAIEAMKTDTQRFQ"
     gene            complement(4279..4710)
                     /locus_tag="VFQ23_03040"
     CDS             complement(4279..4710)
                     /locus_tag="VFQ23_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905580.1"
                     /translation="MVPSRQRVLITSVILFGTAIVCFFGFRTFFAFRNFSGHRPPPLP
                     PAASGQIETDADLIRDWMTIPYISRTYDVPEKLLFDALGISPRGNEEKSLAQLNEEYS
                     QQAPGIVLAVVKGAVRAHQAQIPPEPIKTPIPPSTAVPPKP"
     gene            complement(4712..5977)
                     /gene="obgE"
                     /locus_tag="VFQ23_03045"
     CDS             complement(4712..5977)
                     /gene="obgE"
                     /locus_tag="VFQ23_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET9905581.1"
                     /translation="MFTDQVEIHVKSGKGGDGMVHFRREKFIPLGGPDGGDGGKGGDV
                     IFEVKSTLNTLSAFRQNQKFKAEEGVKGGPSQMSGRYGKDLILHVPPGTILYDADTGE
                     LIGDLTNPGQTLTVLKGGRGGRGNQHFATSRNQAPRTAEKGEPAEEKRIKLELKLIAD
                     IGLIGVPNAGKSTLLSVLTNARPKIAAYPFTTLEPNLGVANIDDDTTVVLADIPGLIE
                     GASHGAGLGHDFLRHIQRTRVLIHLLDGLSEDPVADFSQINSELSLFDPNLGKKPQVV
                     ALNKIDQPEVQERLAEIQKKFKKLKIELMTVSALARTNTRELLIRAHQKLAEAPSLDE
                     IEPPMPVYKPKEDPREFVVTRQGQNEWRVSGTAIELAAKMTYWEHDGSLRRFQKIMET
                     LGVDQALRQAGVQEGDTVAIGDFELEWQE"
     gene            complement(6058..7941)
                     /locus_tag="VFQ23_03050"
     CDS             complement(6058..7941)
                     /locus_tag="VFQ23_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905582.1"
                     /translation="MRDKLFRLRTWLNNVPVTDPVNRRMAALLQVILIGFIAIVFIAV
                     TFTLIISPTSLPPQSAMSRGFIFSLVVGTPFFLIRRGYFRGSVFVIILIFFALETVAV
                     LTAGLHEIAETLTFFTLAIMLAGLLINRIWLLYTYLLSAGVVSLAAYLEQQAGLTEDG
                     IAIAVNFILIHGLMSLFLSQFGITLRTALNDSLEREQELQNEVDVRRQAETTLQQTTE
                     HLEILHEIDRSLLSARSPHEIAMGAIVRIRKLIPCPRASVTLIDLDRNEAKFLAADFD
                     NLKPIADTPISLEEFGINIIEELQLNKPWSTNEILDDPQITDLSRRLAKEDGIHSWLC
                     LPITYQGQLIGSLNLGRGMGNSFSREESIIAHDVANQLAIAIQHINLYNTLQSELTER
                     KKLISELEANNAELERFTYTVSHDLRNPLVTIKGFLGMLEKDLEEGRKDRIASDFRRI
                     GNAADKMDELLSDLLELSRIGRVLNPPEEVDLGRLTREAVELLDARIRSKNVTVNISP
                     ELPIVFGDRIRLREVIENLIDNAVKYTDDHHEPVIEIGASKQKDNHMIFVRDNGIGIE
                     APHLKKIFGLFEKLNQHSEGTGIGLALIKRIIETHGGKIWAESDGLGKGTTFYFTIPE
                     NVK"
     gene            8225..8584
                     /locus_tag="VFQ23_03055"
     CDS             8225..8584
                     /locus_tag="VFQ23_03055"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9905583.1"
                     /translation="MDKTGPESLEPSVFLILNRQIIPLDKKITRLGRQLDNDLVLNEE
                     FVSRFHAEIRFEDNKFVLYDNKSTSGTFVNSQKIERCVLNSGDLISLASIQIMFVNNN
                     ATLVDKARGTTQSLRNK"
     gene            8594..9730
                     /locus_tag="VFQ23_03060"
     CDS             8594..9730
                     /locus_tag="VFQ23_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET9905584.1"
                     /translation="MDRKHTILIIDDEPALLMGLAATIRRHGYNVITATDGNEGLQKA
                     KESLPDLILSDVMMPPPNGFELRKLMNLDPDLASTPFIFLTARSSVEDRVSGMREGAD
                     DYITKPFSTQELLARIEAVFRRVETEQARGREQMRDIAQQDMERLRSEILQNFHHELR
                     TPLVNILMPLELAANNRFSDAEEQSKFLQIALSNADRLESLVTDLILLSNIDHGDLNT
                     IRQPIDVNMHILTLVRKRLERYKSKQLKFSADVPSQGLITAPRREFIHSVLHLVDNAF
                     KFSPEKGLVSLHIKPGSNGGVSILVQDEGDGIPPELREKAFERFYQISQGDSREHEGL
                     GVGLTIARAVFQSLKGDVQILDHQKGCHVQAFIPDRQPDDIVYG"
     gene            9723..11672
                     /locus_tag="VFQ23_03065"
     CDS             9723..11672
                     /locus_tag="VFQ23_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905585.1"
                     /translation="MDEKTIPLSRYQQEYEAASKKKENEWGEDFHYRALFEQTGECVF
                     IIGLDLRYITANQQALRLLGYEPAELVGMPVSKVMSLGEDLAHGNERKTDSDVEERIL
                     KRKDGSVLPVEISASVVYDQDNRPVYIQSIARDISDRKESEETLRRYTRILSVISDAT
                     ARLLRSSNIEARIPEILASLGIATNVSGCAIFEIDTFSTTPSVNIRYWWKNTDSEFDI
                     SSVLLQHVPMILNASGDYSYNTATKDDNPSGFNGLSFVSVPIHGALGSWGFLGLFDNQ
                     KEISWSPSERNTVQTAANLIGSALQRSTYEETIRLNEARNRAILSALPDLLIRINLEG
                     NILDYSANPDHPLYIHRDMISGKKLHETWPSSLVEKIRGPGNQQTFTTSNILEDFRLP
                     FSNKVFESRLYPISPGEGLIVIRDVTDQAQLNEMKSDFINRASHELRTPLTSAILMTE
                     LIQDGGSSEELNEYWRTLKGELNRQKILIDRLLIAGRLESGMMTLERSPLDLLPVLEE
                     SIKAVKAIATKRGVKLILKSERKSVSILGDKSGLQQVFINLINNAVKFSPEGSSVTIN
                     VSEADAKVQVMISDQGLGIPEEAIPHMFERFYRAKNVTIAEIPGSGIGLYIVKSIVDE
                     LGGSIRVESELNKGTTFIVNLQRGS"
BASE COUNT         2910 a         2914 c         2905 g         3124 t
ORIGIN      
        1 taccaatttg ttgtccacca ctttgaacgg gcgccaccag tagtgagcgg aagttaggta
       61 ttgagctgga gagaaggaag cggggatccg tttctacatc cccgatattg attgtgatac
      121 cttcacgaat tacttggcca gccacacctt cgcccagccg catctgcacg cgcttgaact
      181 ctgtgccctg ttcgggatat cccgacacgg cgcgtgccca aagagcttgc gcttctgctt
      241 ctaaaagatg gatgacgctt tcttccgcgt tagaaatcaa ttcacgcgca gagtcgacga
      301 tcagttgcaa gatttgatcg gtcccagtgt gctctgtttc acttaacgcg cgtccgatct
      361 ctgccagcgc gttgctttct ttaagacgat gttgaatctc ggtgctttgc tgaaagttcc
      421 ataacgccaa cgcaatatgg tcggcagcat ggtctgcgcg ttcgatttct tctgaagtaa
      481 agctgtgggg tgtattaaag acgatgattg ccgcccccaa tttatggtcc ccggcgatca
      541 aagggacacc cagcgcagag cgtgccgggt aacgttttgc aacttctgca cttagataag
      601 gcgagttaaa gacgtcatcc gccgcgaggg cccgtccgag ttttaagacg gaggcggtca
      661 gcgttaattg atccgctggc gttcttcgtg ttgggtatgg aatttccagt tttgccgtgg
      721 tggttgtagg aattgttact tcttgttcct catcccagcg cgtgatatag cagtcatcag
      781 cgtcaataag ctttgcgaga tttacagcca gcgtactcaa ggttgagtca aaatcctccg
      841 aaaggaggat ggcgcgcgtc atctcattca ggagtgaaag atatctctcc cgcgagcgca
      901 gatccgattc aacgcgctct tgctcagctt cgtcaggctc tctactcacc ttgctatcat
      961 caagctggct catgtgattt tgctcttggt tatttcctta tttggcgatc tccgtcgagc
     1021 gggtctcacg gattacggtc actcgaatct gaccgggata ttgcatggtt tcttcgatct
     1081 tcttagcgac atcgcgtgcc aatcgtgcgg atgcaagatc atcaatggcg tcgggtttta
     1141 caattatacg gacttcgcgc ccggcttgaa ttgcaaaagc ctgttgaacg ccctcgaacg
     1201 acatggacat ctcttcaagc gagcgaattc gcttgatgta ggcttccagg tcttcgcgcc
     1261 gcgcaccggg tcgtgcgcca gaaatagcgt ccgcggcttc tgcgatgact gcctccactg
     1321 taagttgatc gacctcatga tgatgcgatt cgatggcatt taccacgatg gaatttacgc
     1381 catatcgttt acaaaactcg gcacccagtt ttgcatgtgt gccttcctga ttgtgatcca
     1441 tggctttgcc gatgtcgtgg aggaagccac cttgtttgga aacttctacg ttcgcaccaa
     1501 tttctgcagc aaggatacct gccagcttgg agacttctac cgcgtgggcg agctggtttt
     1561 gtccgtagga ggtgcggaat ttaagccggc ccatcattcg aaggacctca ggatgcagac
     1621 cagtgatatt tgcgtcgaag gcagcctgtt ctccggcttc attgatgatt ttttcgacgg
     1681 cgcgggtttc atcttcgaca atcttttcaa tgtgggccgg gtggattctc ccgtccagaa
     1741 ttaacttggc gagcgcgcgg cgaccgattt cacggcggac cgagtcaaag catgagactg
     1801 tgacagagtc aggtgtatcg tcgacgatga cgtccactcc agcagcctgc tcaaaggctt
     1861 tgatgtttcg tccatttcgg ccgacgatgc gacctttcat ctcctcattg ggcaaggtga
     1921 cgacggcgcg ggtcacttcg gcaacatgct ccgatgcaac cctctggatg gcatctgcaa
     1981 tcaacttgcg agcgcgcttt tcaccttcct cacgcgcttc ggcttcaatc tgacgaataa
     2041 tgcgcgccat atctccgcgc gcttcctttt cggttgcggc gaatagttct ttcctggcgt
     2101 cttctgtcgt gagctgcgca acctgttcga gctttttcag ttgatcttcg tacagcttat
     2161 cgatctcgtt accgcgttta tctacctgcg attggcgttt gttgatcgcc tgttcgcgtt
     2221 gttccagttt ttcgacgcgg ttctcaaact cagaacgacg cttgtccaga cggtccgttt
     2281 ccttacttag ttcaatgcga cgttctttaa tctctgattc ggccgcctgt acgatcttta
     2341 ccgcgttctc gcgtgcctga ctttcgatca gtcgcgcctg ttcgctggcg cctttcaata
     2401 tgttatcggc tctatcctgt tgattcttaa gggctttatc ggcctgataa cggtgtaaaa
     2461 aatagcccgc cgccgcgccc aaacccaggg cgaagagggc tatcagtatt atggttaagg
     2521 tgttcatgtc gtatcctctt cttcaaagtg atgttcatct gtgtgtactt cattccaaat
     2581 acgcgtgacg ataggtgcga tgactgaata agaaaaacct cgacgcgcca ggaattcgga
     2641 gagcttctta cgaaaatcca tccattccag atctttcaat cgattgacct ttttttgagc
     2701 ggcttcataa gccagctctt cgtcatccac ggcttccaac gccgttgaga cagactcgtc
     2761 ggtaacccct ttttggcgca attccatggc catcatccga cggctgcgtg gacgaaaggt
     2821 actgcgattc tccacccagg cctgcgcaaa ttgatcgtcg ttcgcgagac cctcctgcct
     2881 caatcgctga agagtgtgct cgatgatctc atcagggatc tcatgcttgc ggaggttttg
     2941 ccgaatttca gcttcggatc gagaacgata gcttagaaac agcaatgact gttgaactgc
     3001 acgctcacgc gcatcctccg actgcaaccg attgattttt tcgtcatcca actcctgacc
     3061 cgtcttcaac caggcggcga caatgcgcgc cagcgaaaag gcgtattccc catcaatgta
     3121 aatattgacc cggttcgggt tacgcttttg agctgtgaga gcagtaattt tcttcataca
     3181 ctactttgtt cctctcctcg caggagagag aggttaggtg aaaggtggct tcaccctcat
     3241 cccaacccat ctctcatggg gagaagagca ttaaaaaaca aacagccaaa gaaccgccca
     3301 aaggggcaag cggtctcggc tgcggagaat agctgggtag atcgagcaga cccaaagtct
     3361 cgcactttga tgggtgcgcg acagaggcaa tccgtgagat taagtcttag gcacggttgg
     3421 tgaacaggtt gtgggtggtg aagcctggtt ggtaatcata aatttagcag ttgacttcac
     3481 aaaataactc ccaaaacggg ggagattcat gctctaatat ccagggttag tgtacagcca
     3541 gatcgcaaat gcggcagaag tttttttgtt gtcttacaac tttcccctga attatacatg
     3601 aacattaaag atttgttaac cttgcatttg tatggctatt ttggattggc cgaattattg
     3661 aaatctttgg gtatcggttt tcatagcctc aatggctctt aaacgctgga atccgagcca
     3721 aacaccaacg caaagaatga ctaatcccaa ggcgaatcca ccgaaattac ttataaaatc
     3781 actgaccaat tcccattgag tgccgaacag gtagcccaaa ccaccgtagc caaagaccca
     3841 aatcgcttca cccaacacat cgtacatcag aaatcggcga aatggaaagc ttccggtccc
     3901 ggcgatcagg ttcactggga cggcaatccc tgtgatcaga aagcgcgtca ggaagacaga
     3961 catcccaccc cagcgtcgaa agctgttttc tccgtgtgcc cactgacgtg aggcgctaaa
     4021 ccgttgcaat atcggctcac gcgcgtaata acccatggaa taactcaggc agtcacccag
     4081 cacaacgcag attaaggcaa tcaacccggt aatgtgccat tccaaaagac cctgccggca
     4141 aaatgcgccg accgcaacaa caagaaacgt accaggaaag ggcgccccaa tcgctccaat
     4201 aaaaaggatc aatccaagga taggcgcgcc aaaattaata acctgagtca gcagaaattc
     4261 agacattcgc tcttctgctt atggtttggg cgggacggcg gtggatgggg gaatgggagt
     4321 tttgattggt tcaggtggaa tctgggcctg gtgggcgcgt accgcgcctt tgaccactgc
     4381 caaaacaatt cctggggctt gctgggaata ttcttcattt aattgtgcca ggctcttctc
     4441 ttcgttgcct ctgggtgaga tacccagcgc atcgaataac agtttctccg gtacatcata
     4501 tgttctcgaa atataaggga ttgtcatcca atcacgaatc aaatcagcat ctgtctcaat
     4561 ttgcccggaa gcagccggag gcagtggcgg ggggcgatga ccactgaaat tcctgaaggc
     4621 aaaaaacgtg cggaagccaa aaaagcaaac aattgcggtt ccaaaaagaa tgacgctggt
     4681 gatcagaacg cgttgtcgtg agggcaccat atcactcctg ccattccagc tcgaaatcac
     4741 caattgcgac ggtgtcccct tcctgcacac cagcctgtcg aagggcctgg tccaccccga
     4801 gtgtttccat tattttttga aaacgtcgca gagagccatc atgttcccaa tacgtcattt
     4861 tggcagctag ttcgatagct gttcctgaga cgcgccattc attctgtccc tggcgcgtga
     4921 ccacaaactc tcgcggatct tctttcggtt tatacactgg catcggtggt tcgatctcgt
     4981 cgagtgatgg agcctccgcc agcttttgat gtgctctgat taaaagctcc cgtgtattcg
     5041 tacgcgccag ggcagatacg gtcatcagct cgatttttag ctttttaaat ttcttttgga
     5101 tttctgctag tcgttcctgg acctcgggct gatctatttt gttgagcgcc actacttgcg
     5161 gtttcttccc cagattcggg tcaaaaagcg atagttctga attgatctgg ctgaaatctg
     5221 caacgggatc ctctgacaaa ccgtccagta agtggatcaa gacgcgcgtc cgctggatgt
     5281 gacgtaagaa atcatgtccc aatcccgctc catgggaggc gccttcgatg agaccgggga
     5341 tatctgccag cacaaccgtt gtatcatcat cgatgtttgc cacgcccagg tttggctcaa
     5401 gcgttgtaaa tggataagcc gcaatcttgg gcctggcatt tgtgagcaca gagagcaatg
     5461 tcgatttgcc agcgtttggg acgccgatca gcccaatatc ggcgatcagc ttcaattcca
     5521 gtttgatgcg tttctcttcg gccggctctc ctttttcagc agtgcgtggt gcctgattgc
     5581 gggatgtcgc aaaatgctgg ttaccgcgcc cgccgcgccc acctttgagc acggtcagtg
     5641 tctgacctgg gttcgtcagg tctccgatca attcgccagt atcggcatca taaaggattg
     5701 tgcctggtgg cacgtggaga atgagatctt taccgtatcg ccccgacatt tgcgagggcc
     5761 cacctttgac cccttcttcg gctttgaatt tttgattctg ccggaaggca gacagtgtat
     5821 tgagcgtgga tttgacttca aagatgacgt cgccaccctt gccgccgtcg ccgccatccg
     5881 gtccgcccag cggaatgaat ttctcgcggc gaaaatgtac catgccgtca ccgcccttgc
     5941 cagactttac atgtatttcg acctgatcag tgaacatggg tagattataa gcgaccgccc
     6001 tcgccttctg cgcattttca gaatactcgt cgcggtaggg tcagagacgg aagcagatta
     6061 tttcacgttt tctggaatcg tgaagtagaa agtcgtgcct ttccccaacc catcagattc
     6121 ggcccaaatc tttccgccgt gtgtttcaat aattctctta atgagcgcca gcccaatccc
     6181 tgttccttca ctatgctgat taagtttttc aaataagcca aagatttttt ttagatgtgg
     6241 agcctcaatc ccaataccat tgtctctcac gaaaatcatg tgattgtctt tctgtttgct
     6301 cgcaccgatt tctatgacag gctcgtgatg atcatctgta tatttgacag cattgtcgat
     6361 caggttttcg atcacctcgc gtaaccgaat gcgatcgcca aaaacaattg ggagttcagg
     6421 cgagatattc actgtgacat tctttgaccg aattcttgcg tcgagcaatt ccacggcttc
     6481 ccgtgtcagc ctcccaagat caacttcttc aggtggattg agcacacgcc cgattcgtga
     6541 aagctccagc aaatctgaga gtagttcatc cattttatca gccgcgttcc cgattcgccg
     6601 gaagtcgctg gcaattcgat ctttccttcc ctcctcgaga tctttctcca gcatacccag
     6661 aaatcctttg atcgtcacaa gggggttgcg caaatcgtga gacaccgtgt acgtaaagcg
     6721 ctcgagttca gcgttgtttg cttccaattc tgagatgagt ttttttcgtt ccgtcaattc
     6781 agattgcaac gtgttatata aattgatatg ttgaattgca atggcaagct gattggcaac
     6841 atcgtgcgca atgatgcttt cctccctgct aaatgaattc cccattcccc ggccgaggtt
     6901 gagcgaaccg atcagttgtc cctgataggt gattggaaga cagagccagc tgtgtatgcc
     6961 atcttctttc gccagtcttc tactcaaatc tgtaatttgt ggatcatcca ggatctcatt
     7021 cgtcgaccag ggtttgttca gttgaagttc ctcgatgatg ttgatgccaa attcctcaag
     7081 agaaataggc gtgtcggcaa taggctttaa gttatcgaaa tcggcggcca ggaatttggc
     7141 ttcgtttcga tcaagatcga tcagcgttac actggcacgc ggacagggta tcagcttgcg
     7201 aattcggacg attgccccca tggcaatttc atgtggagag cgcgccgaca agagcgacct
     7261 gtcaatttca tgcagtattt ccaggtgttc ggtcgtttgt tgaagtgtgg tttcagcctg
     7321 tcgacggaca tcgacttcgt tttgcaattc ctgttcacgt tccagagagt cattaagtgc
     7381 cgtgcgcaac gtaatgccaa attgactcag aaacagactc atcaatccgt ggatcaaaat
     7441 aaaattcacg gcaattgcga taccgtcttc cgttaagcca gcctgctgtt cgaggtacgc
     7501 agccaaactg acaaccccgg cgctgagcag atatgtgtat aacagccaga tcctgttgat
     7561 caacagacca gcaagcatga ttgccagagt aaaaaaagtg agagtttcag caatttcatg
     7621 caaccctgct gtcaataccg caacagtttc cagagcaaag aaaatgagaa tgatgacgaa
     7681 gacagagcct cgaaaatacc cgcgccgaat caagaaaaag ggagttccaa cgacgaggct
     7741 aaaaataaaa cctctgctca ttgcagattg gggcgggagg ctggtgggag aaatgatgag
     7801 agtaaaggtc accgcgataa aaacaatggc aataaaccca attagaataa cctgaagcaa
     7861 cgctgccatg cggcgattga cagggtctgt gacaggcaca ttgttcagcc aggttcgtaa
     7921 cctgaaaagt ttgtctctca ttggagatcg taggaatgaa gtggattcta tcacccacta
     7981 ccaacttgga agggacaatt gcgcggctct tcttgtccac aaatctgaaa attacgtgat
     8041 tttagaagac aagctcagtc cgatgtcaat attgtatttg taattcttaa cgttgatcat
     8101 gaagatgaaa ttgcaatttt gaggctggtt ttcaaaacct atccgatatg ttataactaa
     8161 ggtatgcggg gagccgttat cgaaatttct tggagtgttt gttttgacat tatggaggcg
     8221 cttcatggat aaaaccggac cagaatctct cgaacctagt gtatttctta ttcttaaccg
     8281 tcagatcatt ccgcttgata agaaaatcac ccggcttgga cggcagttgg ataacgatct
     8341 tgttctaaat gaagaattcg tttccaggtt ccatgcagaa atccgcttcg aagataataa
     8401 gtttgtcctg tacgataaca aatcaacttc gggcacattt gtgaacagcc aaaaaataga
     8461 acgttgcgta ctcaattctg gtgatttaat ttctttggct tccattcaga tcatgtttgt
     8521 gaacaataat gccacgctgg tggataaggc gcgggggacc acacaaagcc tgcgcaacaa
     8581 ataacgcatc attatggatc gcaaacacac gattttaatt attgatgacg aaccagctct
     8641 gttgatgggg ctggctgcga ccatcaggcg tcacggctat aacgttatca ctgcgacgga
     8701 tgggaatgaa gggttgcaaa aggcaaaaga gtctctgcca gacttaatcc tttcggatgt
     8761 gatgatgcca cctcccaatg gctttgaact gcgcaaattg atgaaccttg atcccgattt
     8821 agcatctact ccttttattt ttctgaccgc tcgctcgagt gtagaggaca gagtcagcgg
     8881 catgcgcgaa ggggccgatg actatattac aaagcctttc tcaactcagg aacttctggc
     8941 gcgcattgaa gccgtattca gacgcgtcga gacggaacaa gcccgcggtc gtgaacaaat
     9001 gcgagatatc gctcaacagg atatggaaag actgcggtct gagatcctgc agaacttcca
     9061 tcacgaacta cgaacgccac ttgtcaacat tttgatgcca ttggagctgg ctgcgaacaa
     9121 tcggttcagt gacgcggagg agcagagtaa atttttacaa atcgccctct cgaatgccga
     9181 tcggctcgaa tccctggtta ccgatctaat tttgctcagc aatattgacc atggagattt
     9241 gaataccatc cggcagccga tcgatgtaaa tatgcacatc ctcacactgg tgcgaaagcg
     9301 attggaaaga tacaagtcga agcaactgaa attctcagcg gatgttccaa gccagggtct
     9361 aatcaccgcc ccgcgtcgtg agtttatcca ctctgttttg catttggtgg acaatgcctt
     9421 taagtttagc cctgaaaaag gacttgtcag cctccatatt aagcctggct cgaatggcgg
     9481 cgtcagcatt ttggttcagg acgaaggaga cggcattccg cctgagttgc gtgaaaaggc
     9541 ctttgaacgg ttttaccaga tcagccaggg agacagccga gagcatgaag ggctgggtgt
     9601 tggcttaacg attgctcgtg ccgttttcca aagcttgaag ggtgatgtcc aaattcttga
     9661 tcatcaaaag ggatgtcacg tgcaagcctt tattccagac cgtcagccag atgatatcgt
     9721 ctatggatga aaagaccatc cctctatcga ggtaccagca agaatatgaa gcggcctcga
     9781 aaaaaaagga aaatgaatgg ggggaggatt tccattaccg cgccttattt gaacagacgg
     9841 gtgaatgtgt cttcattatt ggcttggacc tccgctatat cactgcaaat caacaagcac
     9901 ttcgtctttt gggttatgaa ccggctgaac ttgtcggaat gccagtgagc aaagtgatgt
     9961 cgttggggga ggacttggct cacggaaatg aacgaaaaac cgactcggat gtggaagaac
    10021 ggattctgaa gcgaaaagat ggttctgttc tgccggttga aatcagtgct tcggttgtct
    10081 atgatcagga taatcggccg gtctatatcc agagcattgc ccgtgatatt tcagatcgaa
    10141 aggaatccga ggaaactctc aggcgctata cgcgtattct ttctgtcatt agcgatgcaa
    10201 cagcacgttt gctgcgttcg tctaatattg aagcgaggat ccccgagata ttggcgtcgc
    10261 ttggcattgc cacgaatgta tctggctgtg caattttcga gattgatacc ttttccacca
    10321 cgccgtcagt aaatattcgg tattggtgga agaatacaga ctcggagttt gacatttcgt
    10381 ccgtattatt gcaacacgtc cccatgattc tgaacgcatc gggtgattat tcctataaca
    10441 cagcaacaaa ggacgataat ccctccggat ttaatgggct gtcatttgta agcgttccca
    10501 tccacggtgc gctcgggtcc tggggttttt tggggctatt tgacaatcaa aaagaaattt
    10561 cctggtctcc ttcagaacgg aatactgttc aaactgcagc caacctgatt ggctctgccc
    10621 tgcagcgtag tacctatgaa gagaccattc ggctgaacga agcacgtaac cgcgccattc
    10681 tttcggcttt gcctgatctg ctgattcgga tcaatctcga agggaatatt cttgactaca
    10741 gcgctaatcc tgaccatcca ctctatatcc atcgggatat gatttcagga aagaagctgc
    10801 acgaaacctg gccatccagt cttgtcgaga agatcagagg ccccggcaat caacagacat
    10861 tcactacctc gaacattctt gaagatttca gacttccatt ttcgaacaag gtgttcgagt
    10921 ctcgactcta ccccatcagc ccaggcgaag gactgatcgt catccgcgac gtcacggacc
    10981 aggcgcagtt aaacgagatg aaatcggatt ttatcaatcg cgcgtcgcac gagttgcgga
    11041 ctccgctaac ctcggccatc cttatgactg aattgatcca ggatggtggt agctcggaag
    11101 aactaaatga atattggcgc actttgaaag gggagctaaa tcgccagaag attctgatcg
    11161 acagattgct gatcgcgggg cgccttgaga gcggcatgat gactctggaa cgatcaccgc
    11221 tggacctgct ccccgtattg gaggagtcga ttaaggcagt aaaagcgatt gccacaaagc
    11281 gcggtgttaa gctcattttg aagagtgaac gaaaaagcgt gtccattttg ggagacaaga
    11341 gtggattaca acaggtcttt attaatttga ttaataatgc ggtcaagttc tcgccggaag
    11401 gcagttcagt gactattaat gtttcagaag cagatgccaa ggtgcaagtg atgatctctg
    11461 atcagggtct tggaatcccg gaggaggcaa ttccgcatat gtttgaacgt ttttatcgtg
    11521 cgaagaatgt gacgattgcc gagatacccg ggagtggtat cggtttatat attgtcaaat
    11581 ctattgtgga tgaactgggt ggaagtattc gtgtggagag tgaattgaac aaggggacaa
    11641 cgtttattgt caatttgcag cgtggttcgt agcgcaggct tttgctgtca taacgaggca
    11701 acagagttac cccacaaaat gctggtctcg atacgccttc ggctactcga ccatcgaaga
    11761 ccttagaatt ccaaaaaaag gctaaacatt tacgtttagc ctttttcttt acatcacatc
    11821 cggagcttga atatccagca atcggagcgc gtt
//
LOCUS       DASTJP010000100         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_138486, whole genome shotgun sequence.
ACCESSION   DASTJP010000100 DASTJP010000000
VERSION     DASTJP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138486"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFQ23_03070"
     CDS             <1..706
                     /locus_tag="VFQ23_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HET9905586.1"
                     /translation="SASYASYKAEFPGGQYLAIAQQKLDSLNVVSQNKRQSLQSVAEV
                     VTEAPTQVNEDTILEAQTPATPTTPTTKTTPTSKPASQTPAKTPVQQPKKTTSSPVTP
                     PKTKPVEPVTKSDHKLALGQQHNGGIVIFINSTGEHGFVAALKEEGKYSWTAAQRRCG
                     AYKSGGFSDWRLPTRDELGVMYDNRKHLGNYTKGIYWSGTEDGKDIAWGVNFINGSPG
                     KFDKGSANSVWAVHPF"
     gene            955..1974
                     /gene="mgrA"
                     /locus_tag="VFQ23_03075"
     CDS             955..1974
                     /gene="mgrA"
                     /locus_tag="VFQ23_03075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HET9905587.1"
                     /translation="MKIHDSVALPDYEAHKKRYDRIEYRRCGRSGIRLPLVSLGIWHN
                     FGHTDDFMNARKMLRTAFDLGITHFDAANNYGPPFGAAEENLGRILKTDFSSYRDELI
                     ISSKAGWDMWPGPYGDYGSRKYLIASCDQSLKRMGLDYVDIFYHHRPDPRTPLEETMG
                     ALDQLVRQGKALYAGVSQYDAQQTKQASDILKSMGTPMLIHQPRYSMMDRWVEDGLLN
                     VLEEEGIGSIVFSPLEQGLLTDKYLKGFPKDARAVRDGRYLKKDRITPEVLSAIKKLN
                     KIAKDRNQTLAQMAINWLLKDERVTSVLIGASSSEQIIENVKGLKRLKFSKEELDAIE
                     KVLRK"
     gene            complement(2009..>2553)
                     /locus_tag="VFQ23_03080"
     CDS             complement(2009..>2553)
                     /locus_tag="VFQ23_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905588.1"
                     /translation="DSLIVAESTVTFVEHSTYELPVDSVDTMLNITLRDGLLYNSDLM
                     LAEGVPYVVDDSVIRYEYHDRHTIGISDTVVVKMSGKYLVVSSNFTDDELDYWDIVLA
                     EKPNANEMRITGIGNLNPPGNDDRSSNSYKGELSDFERIAPIEKLSDDTFLFRPTPKQ
                     FKKLVKKNLFSEQQTYQVFR"
BASE COUNT          718 a          595 c          608 g          632 t
ORIGIN      
        1 cagtgcatct tatgcctcct ataaagctga atttccgggc ggtcagtacc ttgccatagc
       61 tcaacagaaa ctggattcac tgaatgtagt cagccaaaac aaacgtcaaa gcttgcagtc
      121 tgtagcggaa gtggtaacag aggcaccaac acaagtgaat gaggatacaa ttctggaggc
      181 acagactcct gctacaccca ctactccaac gactaagaca actccaactt caaaacctgc
      241 atcgcagacc cctgcgaaaa cacctgttca gcaaccgaaa aagacgactt cttctcctgt
      301 gaccccgccc aaaacaaaac ctgtcgagcc tgtgacaaaa tcagaccata agctcgcact
      361 tggacaacaa cacaatggtg gtatcgtgat cttcatcaat tccaccggtg aacatggttt
      421 tgttgctgct ttgaaagagg aaggcaagta tagctggaca gctgcacaaa gaaggtgcgg
      481 tgcatacaaa agcggtggat tttctgactg gagattaccg accagggatg aactcggcgt
      541 tatgtatgac aatcggaaac accttgggaa ctataccaaa ggcatctatt ggagtggtac
      601 tgaagatggt aaggatatcg cctggggtgt aaactttatc aatggcagcc ccggtaagtt
      661 tgataaaggt tctgcaaatt ctgtgtgggc ggtacatccg ttttaaagaa gttccatgtt
      721 caatgtccat gttccaagtc agaggcagaa ggcaggaaag ttccatgttt aatgttccat
      781 gttcgatgtt ctggaagaag gcagaaggcc tcccaaaggg agaccagcct ttggctggca
      841 tgaggcagaa ggcgtatggc tctttgcctt ttgaatttgg ctaacggcta atggccaacg
      901 gctaacggcc tgcatttgag gctatttagc agctacttac aagcaactta ctctatgaaa
      961 atccatgact cagtagcatt gccggattat gaagcgcaca agaagcgcta tgatagaatt
     1021 gagtatcgtc gatgcggcag aagcggaatc agacttccac tcgtatcact tgggatctgg
     1081 cacaacttcg ggcataccga tgatttcatg aatgcccgga aaatgctgcg aacagcgttt
     1141 gacctgggca ttacacattt tgatgcagcg aataattatg gtcctccttt tggcgctgcg
     1201 gaagagaacc tgggacgtat tctcaaaaca gatttttctt cttatcgcga cgagctcatc
     1261 atttctagta aggcaggttg ggacatgtgg ccgggaccgt atggtgatta tggctcacgc
     1321 aagtatctca ttgcaagttg tgatcagagc ctgaagagaa tgggccttga ctacgtagac
     1381 atcttttatc accaccggcc tgatccgcgt accccgttgg aagaaaccat gggcgccctg
     1441 gatcagttgg tccggcaagg caaggctctt tacgcaggtg tttctcagta tgatgcgcaa
     1501 caaaccaaac aggcatccga tatcctgaag agcatgggaa cgccaatgct cattcatcaa
     1561 ccccggtata gcatgatgga cagatgggtt gaggatggtc tgctcaatgt ccttgaagaa
     1621 gaaggtattg gctcaatcgt cttctcacca ctcgaacaag gcctgctgac agataaatac
     1681 ctcaaaggat tccccaaaga tgcgcgcgct gttcgcgatg gcagatatct caagaaagat
     1741 cgcatcaccc ccgaggtgct cagtgcgatc aagaaactca ataagattgc caaagaccgg
     1801 aatcaaacgc ttgcacagat ggccatcaac tggttgttga aagatgagcg tgtcacctca
     1861 gtcctgatcg gcgcgagttc ttcagaacag atcatcgaga atgtcaaagg gttgaagcgt
     1921 ctgaaattta gcaaggaaga actcgacgcg atcgagaagg tgcttaggaa ataaaccaac
     1981 gtgatacatc ggtatcatca atccaactct accgaaatac ctggtatgtc tgttgttccg
     2041 aaaacaaatt cttcttcacc agctttttga actgctttgg ggtgggtcgg aataaaaatg
     2101 tatcgtcgct gagtttttcg attggtgcga tcctttcaaa atccgaaagt tcccccttat
     2161 aactattact tgaacggtca tcgttacccg gaggattgag gtttccgata ccagtgatgc
     2221 gcatttcgtt cgcgtttggt ttttcagcca ggacaatatc ccaataatcc aactcgtcgt
     2281 ctgtaaaatt tgaactcacg acaagatatt tgccactcat cttgacaacg accgtgtctg
     2341 aaatgccaat agtatgacgg tcatggtact cgtatctgat caccgagtca tccacgacat
     2401 atggaacgcc ctccgcaagc attaagtctg agttgtatag caaaccgtct ctgagagtga
     2461 tgtttagcat cgtatcaacg ctgtcgacgg gtagttcata tgtactgtgc tcaacgaatg
     2521 tcacagtaga ttctgccaca atcaatgagt cct
//
LOCUS       DASTJP010000101         6269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_674106, whole genome shotgun sequence.
ACCESSION   DASTJP010000101 DASTJP010000000
VERSION     DASTJP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674106"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFQ23_03085"
     CDS             complement(<1..469)
                     /locus_tag="VFQ23_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905589.1"
                     /translation="MNSRIFRNLLFSLILLAYFYYANRYIEATSITVDNEKYYVLFDD
                     AMISMRYAYNLAHGSGLVWNPGERVEGFTNPLWVGFMALFHLFPIAASKISLYIQWSG
                     ALFLGGTLFFIRGLVEQFTDDLLPMLAAVAFTAFYGPLISWNLLGMEVSILTLI"
     gene            606..2351
                     /locus_tag="VFQ23_03090"
     CDS             606..2351
                     /locus_tag="VFQ23_03090"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HET9905590.1"
                     /translation="MLQENPTLTKAKLIDPYGGKLVNLVVEGEEREELIARAAQMPSI
                     KITMRNLCDLELLATGGFSPLTTFMGKADYVRVLREMRLADGTMWPLPITLTADPREL
                     PIVGEELALRNSNFDLIAVMTLDEIYHWDPATEAALAYGTTDHRHPMVSEMERWGKVC
                     ISGPIKVVNLPKYHDFVELRHTPAQVREMLEKMGHENVVAFQTRNPLHRIHEELTKRA
                     AAEVKGSLIVHPTVGMTKPGDVDHYTRVRTYKALVDNHYDKNSTMLSLLPLAMRMAGP
                     KEALLHAIIRRNHGANHFVVGRDHAGPGNDSSGKPFYGPYDAQEVMKQYESELGVKMI
                     PFEMLVYLPDEDRYVEEKDVPKGAKTLNISGTQVRDDYLAKGKLLPEWFTRPETAEIL
                     REMYPPRNKQGFCIWFTGLSGSGKSATTQVLTSLMLERGRELTILDGDVVRTHLSKGL
                     GFSREDRDINILRIGFVASEIARAGGAVICAAISPYRAAREEARKMVGENFIMAYMDT
                     PIEICEERDVKGLYAKARQAMDEGKPMGFTGVDDPYEPPIDPEITLKGYGSSPEENAR
                     IIIKYLEDQGYLNGH"
     gene            2531..4102
                     /locus_tag="VFQ23_03095"
     CDS             2531..4102
                     /locus_tag="VFQ23_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET9905591.1"
                     /translation="MDSRTLLRTASILSLWLLMVTGVLVVLRATPEGSALSDDSIAYV
                     AGARSMLAGQGYREAWLVSNQPVTHFPPAFPFVLVLPGLLGADPLNSARYINALLFGL
                     NAGLLGVLAWRMTPSLTAGAVIAALFVLNSELLQVHTTAMSEPLFIFLSLLALWMFDL
                     YFERHNHWLWLLLCGTFVGMAYLTRYAGLALIATFVVALLILRPTWQKRLKSIGIFLA
                     SVLPWILGWGIRNRLVAENATNRVFAWHPITDENLQIGLRVFSEFFIPIESWRRAAIR
                     QPGIVEGIILIVLVAALVWVIAKAWKYFSEPQQVSAEEKIDAGSREFIPFTSGLYLFA
                     YLASIVAAMTMFDAATKFRLRILSPVFVCLLILLVYLGIWFRNKNRGVVIVLTIVFIA
                     FAFFKQFITVNELSKGGLGYASFQWYDSKAMDYLRELPENVMIYTNEPGAVYLYTGRG
                     TYVLPDRFDTATAQIRSGFEEGVTKMQSEISEGKAVLALFDGGDNAPQDIPILIEGLH
                     LAHKSAGDEIYAANP"
     gene            4141..4968
                     /locus_tag="VFQ23_03100"
     CDS             4141..4968
                     /locus_tag="VFQ23_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9905592.1"
                     /translation="MKIQEKFDLTGRVAVVTGGVGLLGAEFCRTLAESGAAVAVVDLN
                     ASASQSVADTLTKSGYQSLAVPTDIISPVSVNSAVEKIIATFGRLDILVNSAALDPKF
                     DPDAIKKGITPGAFEDYPLDQWNAALNVNLTGMFLMTQACAKQMLAQGKNGSIINICS
                     TYGLNGPDQSIYIKDEERVAFKPVYYTVTKAGVLGFTKYLAAYYAGTEIRVNALTPGG
                     VFNNHEDYFVKNYSAKTILGRMAKKDEMNGALLFLASDASSYMTGNNLVVDGGWTAW"
     gene            4946..6250
                     /locus_tag="VFQ23_03105"
     CDS             4946..6250
                     /locus_tag="VFQ23_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HET9905593.1"
                     /translation="MEGGRHGKSDHGRRTIDHAPECTVNGLIMTHILALIPARGGSKG
                     IPRKNIRNFAGHPLIAWSIAAGLQSNSVNRVLVSTDDEEIAAVAREFGAETPFMRPPE
                     LAQDHTNDLPVFEHALKWLEEIEGYKPEIVVQLRPTSPIRPKNCVDEAVRILIENSDA
                     DCVRGVVPAGQNPHKMWRFKGYDKPMLPLLEVEGINEPYNAPRQVLPPVYWQTGHIDV
                     IRTSTITRKNSLTGRVVYPLVIDARYTVDIDTPADWSKYEALVVSGLEMVTPDNRQHR
                     PMPEKIDLIICDFDGVITDNRVWVDQDGNESIAAYRSDSMRIHEMREMGIEVMILSSE
                     PNRVVAARAKKMGVEAIHGVGIHDKGRVMREILEAKHVHAENVIYIGNDRNDLPCFEI
                     AGWSVAVADAYPEVIHAADHVLTKPGGHGALRELCDLILSKV"
BASE COUNT         1564 a         1532 c         1647 g         1526 t
ORIGIN      
        1 ggatcagggt caggatgctg acttccatcc ccagcaaatt ccaagagatc agcggaccat
       61 aaaaagcagt gaaggctact gccgcgagca tgggaagcag gtcatccgta aactgttcta
      121 caagtccgcg aatgaagaac agcgtacccc ccagaaagag cgcgccgctc cactgaatgt
      181 agaggctgat ctttgaagca gcaattggga agagatgaaa cagcgccatg aacccgaccc
      241 agagcggatt ggtaaacccc tccacgcgct cgccagggtt ccagaccaac ccactaccat
      301 gtgctaaatt gtaggcatac cgcatggaga tcatggcatc gtcaaaaagc acgtaatatt
      361 tctcattatc cactgtaatg ctggtagctt cgatatatct gtttgcataa tagaaataag
      421 ccaacaggat cagggaaaag agcaaatttc gaaaaatccg agaattcatc cgataattcc
      481 ggtgtaacca atattaaaat tcagcgtctg aattatagag tggttacgct ataatcacac
      541 cttgcatacg caaatttgtg atggcgagtt tatcatcact gcaaaataat tataaggagc
      601 aatatatgtt gcaagaaaac cccacgttga cgaaagcaaa attaatcgac ccctacggtg
      661 gaaaactggt aaatctggtc gtagaaggag aagagcgcga ggaattgatc gcgagagccg
      721 ctcaaatgcc ttccatcaag atcaccatgc gaaatctgtg tgaccttgaa ttgcttgcca
      781 ccggtggctt ctctccgctg acaaccttca tgggcaaagc ggattatgtt cgtgtgctgc
      841 gcgaaatgcg cctggcagac gggacgatgt ggcctctccc gattacattg acagctgacc
      901 ccagggaact gccgatcgtc ggtgaagaac tggctctgcg aaattccaat ttcgacctga
      961 tcgctgtaat gacccttgat gagatttacc actgggaccc ggcgaccgaa gcagccctgg
     1021 cttacggcac aacagatcat cgccacccga tggtttctga aatggaacgc tggggcaagg
     1081 tctgcatctc cgggccgatc aaagtagtga acctgcccaa atatcacgac tttgtagaac
     1141 ttcgacacac acctgcccag gtgcgtgaaa tgctcgagaa gatgggccat gagaacgtcg
     1201 tcgccttcca gacccgcaat ccgctgcacc gcattcatga ggaactgacc aagcgcgccg
     1261 ccgccgaagt caaaggctca ttaattgttc atccgactgt aggtatgacc aagcccggcg
     1321 atgtggatca ctacacccgc gtccgcacct acaaggcatt ggtggataat cactacgata
     1381 agaacagcac tatgctcagt ttgctgcccc ttgcgatgag aatggctgga ccgaaagaag
     1441 ccttactgca cgccatcatt cgccgcaatc acggtgcaaa ccatttcgtc gtgggacgcg
     1501 accacgcagg tcccggaaac gattcctccg gcaagccgtt ctacggtccg tatgatgcgc
     1561 aggaagtgat gaaacaatac gaatccgaac tgggcgtcaa gatgatcccg tttgagatgc
     1621 ttgtttatct ccccgacgaa gatcgttacg tggaggagaa ggatgtgccg aagggtgcca
     1681 agactctcaa tatctccggc acgcaggttc gcgatgatta tctcgccaaa ggcaaattgt
     1741 tacctgaatg gttcacccgc cccgagacgg ccgagatcct gcgcgagatg tacccaccgc
     1801 gcaataaaca gggtttctgc atctggttca caggcttgag tggttcaggc aagagcgcca
     1861 cgacacaggt gttaacgtcg ctcatgctcg aacgcggacg tgagctcacc attctggacg
     1921 gtgacgttgt tcgcacacac ctttccaagg gacttggttt cagtcgcgaa gaccgcgata
     1981 tcaacatcct tcgcatcggc tttgtagcaa gcgaaatcgc ccgggctggc ggcgcagtca
     2041 tttgcgcagc aattagcccg taccgcgcgg ctcgtgagga agctcgcaag atggtcggtg
     2101 agaatttcat catggcctat atggacaccc ccattgagat ttgtgaagaa cgcgatgtga
     2161 aaggcttata tgccaaagct cgccaggcta tggatgaagg caaaccgatg ggctttacag
     2221 gtgtggatga tccgtatgag ccaccgattg accctgagat cacactcaag ggttatggct
     2281 catcccctga agagaacgca cgcattatta tcaagtatct ggaagatcag ggttacttga
     2341 acggtcacta atcaagaagc attacagagt gccgcataag taggttaagc ttaggcttga
     2401 attctggtct acaagcgaag cagtccggcg gacaaagcat tacaaatagt ttcttaatta
     2461 acagataagc ggtaaatgtg gtatatgtgg gaggggcggg ataacccgcc tctcccattt
     2521 tgcttttttt atggactctc gcaccctgct tcgcaccgcc tccattctaa gcttgtggct
     2581 gcttatggtc accggcgttc ttgttgtact gcgagctacc cctgaagggt cggcgctttc
     2641 tgatgattca atagcatacg ttgccggcgc ccgcagtatg ttagccgggc agggatatcg
     2701 cgaagcctgg ctggtttcca atcagcctgt gactcacttc ccacctgcat ttccctttgt
     2761 cctggtattg ccaggcttgt tgggcgcgga ccctcttaac agtgcgcgtt atatcaacgc
     2821 gttgttgttc ggcttgaatg ctggtttact cggtgttctg gcctggagga tgactccctc
     2881 actaacagcg ggagccgtga ttgccgcgtt gttcgtatta aacagcgagt tgcttcaagt
     2941 tcatacaacg gctatgagcg aaccgttatt tatctttttg tctctgctgg cgctgtggat
     3001 gttcgatctt tacttcgaac gtcacaacca ctggttgtgg cttcttctct gtgggacatt
     3061 tgtgggtatg gcctatctca cgcgttatgc agggctggca ctgatcgcga cgtttgttgt
     3121 ggcattgtta attttgcgcc ctacctggca aaagagactt aaaagcattg gtattttcct
     3181 tgcctctgtg cttccatgga ttcttggctg gggaattcgt aaccgtcttg tggcggagaa
     3241 tgcaacaaac cgagtgtttg cctggcatcc gattacagac gaaaatttgc aaatcggatt
     3301 gcgcgtattc tcagagtttt tcattccgat tgaatcatgg cgcagggcag ccattcggca
     3361 acccggtatt gtggagggaa tcatccttat tgtccttgtt gcggcgctgg tctgggtcat
     3421 tgccaaggca tggaaatatt tttcagagcc tcaacaggta tctgctgaag aaaaaataga
     3481 tgccggatca cgagagttta ttccatttac cagtggcttg tatctctttg cctatcttgc
     3541 atccatcgtt gcggcgatga ccatgtttga tgccgcgacc aaattcaggc tgcgtatctt
     3601 atctcccgtt tttgtttgtc tgttgatcct gctggtttat cttggcattt ggtttcgcaa
     3661 caaaaatcgc ggtgtagtca ttgttctcac gattgtattt atcgcttttg cattttttaa
     3721 acaattcatc acagtcaatg aattatccaa aggtgggctc ggatacgcct ccttccagtg
     3781 gtacgattcg aaagccatgg actatctgcg tgaattgccc gaaaacgtga tgatctatac
     3841 gaatgagccc ggcgcggtct atctttacac ggggcgcggg acgtatgttc taccagaccg
     3901 attcgacact gccaccgccc aaatccggtc agggtttgag gagggtgtaa caaagatgca
     3961 atcagagatt agcgagggga aggctgtgtt ggcgctgttc gatgggggcg ataacgctcc
     4021 ccaagatata ccgattttga ttgaaggatt gcatctcgcg cataaatctg ccggtgacga
     4081 gatctatgcg gcaaatccat agtgcacatc ggtgactgtc accgaaacat aggagaatga
     4141 ttgaagattc aggaaaaatt cgacctgacc ggtcgtgtcg ccgtggtgac gggcggggta
     4201 ggtttgctcg gtgcagaatt ttgcaggaca ctcgccgaat caggcgcggc ggtcgccgtg
     4261 gtcgatttga acgcatcggc ttcgcaaagt gtagctgata cgctcacgaa aagcggatac
     4321 cagtctctgg cggtgcctac cgatatcata agtcccgttt cggttaattc tgccgttgaa
     4381 aaaatcattg ccacatttgg gcgattggat attttggtca atagtgcagc acttgacccc
     4441 aaatttgatc ccgatgcgat caaaaaagga attacgccag gtgcgttcga agactatcca
     4501 cttgatcagt ggaatgcagc actgaatgtg aatcttacgg gcatgtttct gatgacacag
     4561 gcctgtgcaa aacagatgct ggctcagggt aaaaacggaa gcattatcaa tatctgctcc
     4621 acctatgggc tgaatggtcc tgaccagagc atttacatca aggatgaaga gcgcgtggca
     4681 ttcaagcccg tctattacac agtgaccaag gcaggagtgc tgggttttac caaatatctg
     4741 gctgcgtact acgccgggac agagattcgg gtgaatgcgc ttacccccgg cggcgtgttc
     4801 aataatcatg aagattattt cgttaagaat tattcagcaa aaaccattct tgggcgcatg
     4861 gcgaagaagg atgaaatgaa cggcgccctg ctgtttttag cctctgacgc ttcatcctat
     4921 atgacgggga ataaccttgt cgttgatgga gggtggacgg catggtaaat ctgaccatgg
     4981 acgacggacg atcgaccatg ctcccgagtg tacggtcaat ggtctcatta tgacccacat
     5041 cctggccctc atccctgcac gcggcggctc gaaaggaatc cctcgcaaga acatccgtaa
     5101 ttttgccggt catccgttga tcgcctggag tatcgcagcg ggtttacagt ccaatagtgt
     5161 caaccgcgtc ctcgtttcta cagatgatga ggagatcgcg gcagtggcgc gggaattcgg
     5221 agcggagact cccttcatgc gtccgcctga gcttgcccag gaccatacca atgacctgcc
     5281 cgtttttgag cacgccttga aatggttgga agagatagaa ggctacaaac ccgagattgt
     5341 tgtacaattg cgtcccacct cgccgatccg tcccaaaaat tgcgttgatg aggcggtcag
     5401 gattttgatc gaaaactcag acgctgattg tgtgcgcggc gttgtgccag ccggtcaaaa
     5461 ccctcataag atgtggcgat ttaaaggcta tgacaagccc atgctgccgc tgctggaagt
     5521 tgagggcatt aatgaacctt ataatgcgcc acgccaggtc cttccgccgg tctattggca
     5581 gacagggcac attgatgtca tccgtacatc gacaattacc agaaaaaact cattgaccgg
     5641 gcgtgtggtg tatccactgg taattgacgc gcgttatacg gtggatatcg atacacctgc
     5701 agactggtca aaatatgaag cgcttgtcgt cagcgggctc gaaatggtaa cgcccgataa
     5761 caggcaacac cgcccaatgc ccgaaaagat cgacctcatc atctgtgact tcgatggcgt
     5821 gatcacagat aaccgcgtct gggtggacca ggatggaaat gaatccattg ccgcgtatcg
     5881 cagtgatagt atgcgcatcc acgaaatgcg tgagatggga atcgaagtga tgatcctttc
     5941 ctcggagcca aatcgggttg tggcggcgcg cgcgaaaaaa atgggtgtgg aagcgattca
     6001 tggggttggc attcacgata agggtcgcgt gatgcgtgaa atccttgaag cgaaacatgt
     6061 tcatgcagag aatgtcattt atatcggcaa cgatcgaaat gaccttccct gctttgaaat
     6121 cgcaggctgg tcggtggctg tggcagatgc atatcccgaa gtgatccatg cggcagacca
     6181 cgtcctcaca aagccaggcg ggcacggcgc gttgagagaa ttatgtgatt taatattgag
     6241 caaagtttga agataagagg cctaagaaa
//
LOCUS       DASTJP010000102         4669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1405915, whole genome shotgun sequence.
ACCESSION   DASTJP010000102 DASTJP010000000
VERSION     DASTJP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405915"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..>4669
                     /locus_tag="VFQ23_03110"
     CDS             28..>4669
                     /locus_tag="VFQ23_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9905594.1"
                     /translation="MTNTLSVRQKSVLIPDTRVSDPALPAWNGSSPVKSYLGLSLVAD
                     NQMVGTLEIGHLSPGTLGQTDLELVQLISSQAAYSVRNSILYTDEQVRSAELSGLANL
                     AQAFTAAPDYTNLISRLIETISPLFSVEILGFLLYDESKRTLEGQIPFQGLPRHIVEI
                     YRTNVEPESTAEKLLLNREMFVTQDAASDAAWHALGLQNLAQAASLRESLLVPLVTGQ
                     RFVGYLQFSNHREAATEFSESEIRLAQTVANQAAGIIENSFIVEKTQQQALRSDALRR
                     LANLATSTAPLDEIMRFSVQELVQLFQGDIGAVFLLDEQIGELRLHRESVVGASADSA
                     GPLARLHVDEPQYRYTVSGSQKPFLSGRLSSDRRILPVYRPLVNTLQVESAIVVPLLI
                     RDRSLGELMLGSRKAEFFNNYDLQVISTAAGQLASAIEDATRATQTDESLRRRVEQLT
                     SIARVSRELNSMTDLKSLLEVVRDESLRATGADCGAILLFETNSSINPPPVSLALGCQ
                     LPDVLPELDRTAIESGEPQLVNNYAEGGKTPIHEGIRSALVVPIFSQGKTVGLIHLHS
                     TKPEFFDQTSLDIAQTLASHAAVALGNVQRFQEQRQRTELLRRRADTLAKLTEVNYAL
                     NFEQPLEQLLRAIANSIREATPFQVALVSVYEPESGLLRRVTGVGFTSETLVELMARK
                     QPLSSVQQMLKPQFRISRSYFIPIDEAPIIPADVHMVTLEQDDKSRKSPNRWDPDDIL
                     IVPLTDSQNAPLGLMSFDKPRDGLRPDRATIETLEIFAAQASLAIANHSRFIQLRTKA
                     EALTAGMERQQRLIKLTQNDLPILLRKDLDQTIAIQNLDQRGQRVRAGLAITESVSRQ
                     LDASSALQALGRETLTQLGMSVAMVAETSGEGPRLLHVLGSVPRATSPDALFGQRNPL
                     RTCLQNGEAILISNLDENLEWRETPLLSALRAKALICLPIRIDKQTVAAMLAVSPEPM
                     PAFTDEDLQVYHQIARQTSVILQNISLLSETRRRLQEVNLLLDFSRQLRGLDAERIVK
                     ALLESARRALNSAHAGVVLIWDEHGNQLSPHAVSGYADNETMKRIAYHAGESLPGQVF
                     ETKRPMRVDEVQFTRDYVLSTESLVLYRQATGGRLPVSSLLIPIQSGDQSVGVLVLDN
                     FNTPSAFTQEDETLLLSLSKQVGLSLQNVRLIQTIQERATQLESLTNAATTLTASLQS
                     DELVTSLLDQLEPVIPYDTATLWLRDKNRLNVAAARGFPDTERRLGLTIAVDDSALFK
                     EMIRTGQVILVDDVRQDPRFPQLEAPRLSWLGMPLIAKNEVIGVLALEKWQAYFYNRE
                     NLQVGTTFASQAAIALENARLFEDSMSRASELDQRSQRLALLNRFSSALSGLLEEGKI
                     LQLTAQELMDALNAPLVSVVMFERGNPVWRHALPKTKAKLPQVLPDAPIFRRLQESLG
                     VFVTDSVGTESDLVSLESFLGEETKSLLILPLVSGANLRALIFIHQNESMRFRLTEIE
                     LARTLTNQASIALENARLFQTTASTAERFSALNQASYQVGANLDPEQIYVAVH"
BASE COUNT         1072 a         1209 c         1216 g         1172 t
ORIGIN      
        1 cgagcgatgc gttcccaatt tggagaattg acaaacacgc tgagcgtccg tcaaaagtcg
       61 gtgctcattc cagatactcg agtgagcgac cctgctttgc ccgcctggaa tggaagttcg
      121 cctgtcaaat cctatttggg actgtccctg gtcgccgata accaaatggt tggcacgctt
      181 gaaattgggc acctttctcc cggaacgctc ggccaaaccg atctggaact tgttcaactc
      241 atttcttctc aggcggcgta tagcgtccgc aactccatcc tctatactga tgaacaggtc
      301 cgctcggctg agttaagcgg gttggcaaat ctggctcagg ctttcactgc tgctccggat
      361 tatacaaacc tcatcagccg tctgattgag acgatttcac cgttgttttc agtcgagatc
      421 cttgggtttt tactctatga tgaaagtaaa cgaaccctgg aagggcaaat tccatttcag
      481 ggactgccca ggcatatcgt tgaaatttat cgtactaacg ttgagcccga gagtacggcg
      541 gaaaaattgt tgttgaatcg agaaatgttt gtcacacaag atgccgcttc ggacgcggcc
      601 tggcatgccc tgggcttaca gaacctcgct caggcggcaa gccttcgtga atctctgttg
      661 gtcccacttg tcaccgggca aagatttgta ggctatcttc aattttcaaa tcatcgagag
      721 gctgcgactg aattttctga atcggaaatc cgactggcgc aaactgttgc aaatcaggcc
      781 gcagggatta ttgagaattc attcattgtg gagaagactc agcaacaggc gttacgctct
      841 gacgcgctgc gtcgtctggc aaacctggcg acctccacag caccgttgga cgagatcatg
      901 cgtttctccg ttcaggaact ggttcagcta ttccagggcg atatcggcgc agttttcctt
      961 ctcgatgaac aaattggaga gttgaggctg catcgtgaat ctgtggttgg tgcatcggct
     1021 gattctgccg gtccacttgc gcgtcttcat gtggatgaac ctcaatatcg ttataccgtc
     1081 tcaggaagcc aaaagccatt tctttccgga cgattaagtt cagatcgccg gattttgcca
     1141 gtttatcgcc ctttagtgaa cacccttcaa gttgaatctg ctatcgtcgt ccctctccta
     1201 attcgggatc gcagccttgg cgagctcatg ctggggagtc gtaaagcgga atttttcaat
     1261 aactatgatt tgcaggtgat ttcaaccgct gcaggtcaac tggcttcggc gatcgaagat
     1321 gctacacggg ctacgcagac tgatgaaagc ttgcgtcgac gtgtcgaaca gttgacctcg
     1381 attgcacgtg tcagtcgcga actgaattcg atgactgacc tgaaatctct tctcgaagtt
     1441 gtgcgtgatg aaagcctgcg ggcaacaggt gcagactgtg gtgcgatcct tctcttcgag
     1501 acgaattctt caataaatcc accacctgtt tcattggcgt tgggttgcca gttgcccgat
     1561 gttctccccg aacttgatcg tactgcgatt gagagtggcg aaccacaatt agttaataat
     1621 tatgcggaag gcggtaaaac gcccattcac gaggggatac gttctgcact ggtggttccc
     1681 atctttagtc agggcaagac cgttgggtta attcatctgc attccacgaa acctgagttc
     1741 tttgatcaga cctcactgga tatcgcacag actcttgctt cgcatgctgc agtggcgctg
     1801 ggaaatgtgc agcgctttca ggaacaacgc cagcgtactg agcttctgcg ccgtcgtgcc
     1861 gataccttgg caaaattgac ggaggtcaac tatgctctga atttcgagca gcctttagag
     1921 caattgttac gcgcgattgc aaatagcatt cgagaggcca cgccgttcca ggtcgcattg
     1981 gtcagcgtct acgaacccga atcgggattg ttgcgtcggg taaccggagt cgggtttacc
     2041 tccgaaaccc tggttgaatt gatggcgcgc aagcagcctc tttcgagtgt gcagcaaatg
     2101 ctcaaaccac agtttcgcat cagtcggtcc tacttcattc ccattgatga ggctccgatc
     2161 ataccggctg atgtgcatat ggttaccctg gagcaggacg acaagtccag gaaatctcca
     2221 aaccgttggg atccagatga tattctcatc gttccactca ccgatagcca aaacgccccg
     2281 cttggcttga tgagttttga taagccccgc gatggattgc gccccgatcg tgcgacgatc
     2341 gaaacacttg aaatctttgc ggctcaggca tcccttgcga ttgcaaacca ttcgcgtttc
     2401 atccaattgc gcacgaaagc ggaagcattg acagccggga tggagcgaca gcaacgcctg
     2461 atcaagctca cgcagaacga tttacccatt ctgcttcgca aagatctgga tcagaccatt
     2521 gccatccaaa acctcgatca aagagggcag agggtgcggg cgggtttggc gattacagag
     2581 tctgtaagcc gtcaactgga tgcatcctcc gcgctgcaag cccttggccg tgagacgctt
     2641 acccagttgg gaatgtcggt tgccatggta gctgaaacga gcggcgaagg accgcggctg
     2701 ttgcacgtgt taggaagcgt tccccgagct accagcccag atgcactttt cgggcagcgg
     2761 aatccgcttc ggacgtgtct gcagaacggc gaagccatcc tgatctcgaa tctggatgag
     2821 aacctcgaat ggcgtgagac gccgctcctc agtgccttac gagccaaggc gcttatttgt
     2881 ctcccgatca gaattgataa acaaacagtg gctgccatgc tggctgtgag ccctgagccc
     2941 atgccagcct ttaccgatga agatttgcag gtatatcacc agatcgcgcg gcaaacttcc
     3001 gtgattttgc aaaatatctc cctgttgagt gagacgcgcc gtcgcctgca ggaagtgaac
     3061 cttctgcttg atttcagtcg ccaattgcgc ggattggatg cagaacggat tgtcaaagca
     3121 ctgcttgaaa gcgctcgccg cgctctaaat tccgctcatg ctggcgtcgt gctgatctgg
     3181 gatgagcacg ggaatcaatt gtcgccgcat gctgtttctg gttatgcaga taacgaaacc
     3241 atgaaacgca ttgcgtatca cgcgggcgag tccttgcccg gacaggtatt tgaaaccaag
     3301 cgacccatgc gtgtggatga agtccaattc acgcgagact atgtcttatc cactgaatcg
     3361 ctggtgctct accgacaagc aactggcgga cgtttgccgg tttcaagttt gttgattccc
     3421 attcagtcgg gcgatcaatc agttggtgtt ttagtgttgg ataatttcaa taccccgtca
     3481 gcgtttacgc aggaagatga aactctgctc ctgtcactct ctaaacaagt tggcttgtca
     3541 cttcaaaacg tccggctgat tcaaacgata caagaacgtg ctacccaatt ggaatctctc
     3601 acgaatgctg ccacgacgct cactgccagc cttcagagtg atgaactggt gacatcgctt
     3661 ctggatcaac ttgagccggt cattccttac gatacggcaa ccctgtggct tcgagataag
     3721 aaccgcctca acgtggcggc ggcgcgcggc ttccccgata cggagcgtcg cctgggtttg
     3781 acgattgcag ttgacgacag tgccttgttc aaggagatga tccgaaccgg gcaggtgatt
     3841 cttgttgacg atgtgcggca ggatccgcgc ttccctcaac tggaagcccc acgcctctcg
     3901 tggctcggga tgcctttgat tgccaagaat gaggtaatcg gtgtgctggc gcttgagaag
     3961 tggcaggcgt acttctacaa tcgcgagaac ttgcaggttg gaacgacctt tgcaagtcag
     4021 gcggcgattg ctttggaaaa tgccagactg ttcgaagata gtatgagccg tgccagcgaa
     4081 ctggatcaac gttcacaacg tctcgcactg ctaaaccgtt tttcgtctgc acttagcggc
     4141 cttctagaag aaggcaagat tctgcaactg actgcccagg aactcatgga tgcccttaat
     4201 gctccactgg tttcggtagt gatgtttgag cgcggaaatc ctgtatggcg acatgcattg
     4261 ccaaaaacaa aggcgaagct tccgcaggtt ttgccagatg caccgatctt tcggcggttg
     4321 caagagtcgc ttggggtgtt tgtcactgac tcggttggga cagaatcgga cctggtttca
     4381 ctggaaagtt ttctgggtga agagaccaaa tcgcttctta tcttgccact ggtaagcgga
     4441 gcaaacctgc gtgccctcat cttcattcat caaaatgaat ccatgcgctt ccgtctgacg
     4501 gagatcgaat tggcacgtac gctcacgaat caggcatcga ttgctttgga aaatgcacgt
     4561 ttgttccaaa ccacagcttc tacagccgaa cgcttctctg cccttaacca ggcgagctat
     4621 caagttggcg ccaatcttga tcctgaacag atctatgtgg cggttcata
//
LOCUS       DASTJP010000103         7282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_451619, whole genome shotgun sequence.
ACCESSION   DASTJP010000103 DASTJP010000000
VERSION     DASTJP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451619"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..930)
                     /locus_tag="VFQ23_03115"
     CDS             complement(34..930)
                     /locus_tag="VFQ23_03115"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9905595.1"
                     /translation="MNRKEFGELIATLRQDLDWKQSQLAELANVDDAVISQIERGVKK
                     HFEPELLFQLANVFQLTTLERREFFLAASGLDQREIVRQITVSMNTDVFNARKVLDKM
                     IETTGQVRLPAFLCDVYSDVIATNHIVLEFFKVPPSFVEESSRVPGGYNTTRFNFGNG
                     LVARSHILDNWDSYALKSMRAFRENSLRYRAKPYFKYLMKTFRNPHEYPLFDRYWRMV
                     STVEQDKEANTDYFSFHHDEFGEIKYSTSSTVSVTSFGELFLVHYLPLDAHTSQVFES
                     LTQQAGPGVVQFAPWPLKTFPH"
     gene            complement(1155..3872)
                     /gene="ppc"
                     /locus_tag="VFQ23_03120"
     CDS             complement(1155..3872)
                     /gene="ppc"
                     /locus_tag="VFQ23_03120"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET9905596.1"
                     /translation="MKDPNKIEISRIIHLLGDVLGEVISELESPELFAIEERIRLASK
                     DRRDGDAKAAKQLETDVEALDINQARGVSAAFTTYFELVNLAEEQIRVENLRERILSN
                     EPPGESILDAIAQLKKEGINYRQMQDMLDDLSIELVLTAHPTEARRRTVVSKLQRLTR
                     LLDEISRKPISDHQETEIRASIHAEVASLWLTDVHRTERPQVEDEVRIGLQFVESVFW
                     DTLPEIYDDLHDALASHYPDVVAPSNWLRLASWMGGDRDGNPNVDHTVTAETLRLHRG
                     LAVEQHRERLQDLARRLSISSRRLSPQPDLIKWIESRGKLPDHVAYIQKRYPDEPYRL
                     VLSLLATDLAEASKDDMTARLLERKPHEARMKLPDLLEPLDFISAGLPASIAQDEIEK
                     VRYQLHIFGLHSMRLDIREESSRYNAALGETLRALNITPNFAEMPDAERLSFLTEMLS
                     SSLPDLAEHPGITAATAETWALFQLIKRVRDVYGAELLGPVVISMCRSAADVLTVLLL
                     ARWAGCDHELQIVPLFETIEDLQDASSILESLFTLELYREHLAKCGNQQMVMIGYSDS
                     NKDGGYVMANWALYQGQENITQVAQKHNVTLTIFHGRGGTIARGGGPANRAIRAQPPG
                     SINGRFRLTEQGETISSRYSNPELAHRHLEQIVHAVLVASSPANSRAEIPGSWRAVMD
                     EMSSVAFRAYRGLVYETPAFIDFWQAATPLDEIKSMHIGSRPAARTKSSEVSKIRAIP
                     WVFSWMQSRFNLPGWFGLGTALESIQNPALLQDMYQGWAFFKSMLDNTEMSLLKADMD
                     IAALYVDLVPDKAMATEIFSLIKAEYDRTSKAVLAISGHQNLLDAERITQHAIQLRNP
                     YVDPLNYIQVEMLRRLRALPESEGAEAQSLREVIRLTINGIAAGLRNTG"
     gene            complement(4141..5139)
                     /locus_tag="VFQ23_03125"
     CDS             complement(4141..5139)
                     /locus_tag="VFQ23_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905597.1"
                     /translation="MEFDLLIIGLASVLFAAAPIVFAVIGETLSERAGVINLSMNGTI
                     LLSAMGAFGVSVRTESLLLGFLTGAAIGALVALIVAFSSITLKQSQVAVGFVLALVCR
                     DLSYFLGNPIMGAAGPRVPALPLPGLSQIPVLGPLLFNQDVITYLSYLIIFVSWVWIF
                     RTHPGLMLQGIGERPAASYVRGANVNLMRYVYTVIGGAIIGLAGPAYSLSVKAGWKGT
                     ISGLDGIGWIALAITIFGGWNPLRAAFGAYLFALLQWLGLVLQPSLPGVPSQVLQVAP
                     FPLMILTLLLVNIGNADWVDRTLASLPESTRRFLARAFRSMRASPPAALGVPFERD"
     gene            complement(5146..6186)
                     /locus_tag="VFQ23_03130"
     CDS             complement(5146..6186)
                     /locus_tag="VFQ23_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905598.1"
                     /translation="MTKNARWTNFGFQIAAFVLALLFTTLILILAGAPPFEAYKNIVI
                     GSVGSFKKFTDVLVSWVPLLLVSAGLLITFSAGLWNIGVEGQITLGAIGTTWALRALQ
                     DSSLTPGLIILFSILAGMLAGALWAALAGALKTFGGVNEIFGGLGMNFVATAMTLWLI
                     FGPWKREGIGSMSGTEPFDETLWLPVMTGLRLSPWALGLAIIAIIVVYFLLQGTYFGL
                     RLKAVGKNFRAAYMLGIPTWQYLMVSFILCGLLAGAAGAMQVTAVYHRLIPSISSGYG
                     YLGLLVAMLVNYQAIWAVPVALFFAALNIGSIQLPIVLQLDSSLAGVLQGMLVLFVLI
                     VQGVRQQFFRRS"
     gene            complement(6191..>7282)
                     /locus_tag="VFQ23_03135"
     CDS             complement(6191..>7282)
                     /locus_tag="VFQ23_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9905599.1"
                     /translation="PDSYVDLLTVGERQQLEILRLLWLGARVLILDEPTTGISASQKE
                     KLFLTLINKLGVQGMTILFVSHKLEDVEILSDSIAVFRQGKLVGEAKRPANTEKLVAM
                     MFGKNITIGGRQAMGQEKNVLSLNDVCIEDSRLRIQDLNVQIRAGEVIGLAGLEGSGQ
                     SSFLRACAGLIRPVSGQVKLNDRDFTGKTYHTFKSNGVEFLPAARLEEGLVPGLTLTE
                     HFVLAGKQKGVFIDRESGRHVTEERIKAFNIKGTPVSPIESLSGGNQQRALLSLLSDP
                     LNIALIEHPTRGLDIESAIYVWGKLKERCKQGAAIVFISSDLEEILQYSDRVLVFFSG
                     KVSPPQDAAQTSVEQLGQLIGGKGWDEKD"
BASE COUNT         1943 a         1914 c         1743 g         1682 t
ORIGIN      
        1 attgctaggc atgggtagaa ttaatagctg aatttagtgt ggaaatgttt ttaggggcca
       61 gggtgcaaac tgaaccacgc caggacctgc ttgctgtgtg aggctttcaa agacttgact
      121 ggtatgggca tccaaaggca aataatgaac caggaacagt tcaccaaacg acgtgaccga
      181 aacggttgac gacgtgctat attttatttc cccaaactcg tcatggtgaa aggaaaaata
      241 atctgtattc gcttctttat cctgttccac cgtggatacc atcctccaat agcggtcaaa
      301 aagtggatat tcatgcgggt ttcgaaacgt tttcattaaa tacttgaaat aaggttttgc
      361 gcgataacgc agactatttt cacgaaatgc gcgcatggat ttaagtgcat aactatccca
      421 attgtctaaa atatgacttc gagccaccaa gccattccca aaattgaaac gcgtcgtatt
      481 gtatcctcct ggaacgcgac tggactcctc tacaaaggaa gggggaactt tgaaaaactc
      541 caatacaata tgattggttg cgattacatc gctatagaca tcgcaaagaa aggcaggcag
      601 ccgtacctgc ccggtcgttt caatcatttt gtccagcact tttctggcat tgaaaacatc
      661 agtattcata gacacagtga tctgtctgac gatctccctt tgatccaagc cgctggcagc
      721 caggaaaaac tcgcgccgct caagcgtcgt caactggaag acattagcaa gctgaaacag
      781 aagttccggc tcaaaatgct tctttacgcc acgctcgatc tgactgatga ctgcatcatc
      841 cacattggcg agttccgcca gctgagattg cttccaatcc aaatcctggc gcaaggttgc
      901 tataagttct ccaaattcct tgcgattcat ctagaccttt tgaccaactt gactcgaaaa
      961 tatttttgag tcaattctaa atcagattac tggactttgc cagacaaatt cgtcataata
     1021 attttagaat tgacccggga tatcttattg acccggatta ataaaagggc ggatccccag
     1081 gagggggatg agagccagaa aaaggactcc agttgtactg gagtcctttt ctttaagccc
     1141 gggtggatat ttgtttatcc ggtgtttcgt agaccagccg caattccatt gatggtcaag
     1201 cggatgactt cgcgcaaact ttgggcttca gccccttcag attccggcaa ggctcgtaaa
     1261 cgccgcaaca tttcaacctg aatatagttc aacggatcca cataggggtt acgcaactgg
     1321 attgcgtgct gcgtgatccg ttctgcatcc agtaaattct gatggccact gattgcgaga
     1381 acggccttgc ttgtcctgtc gtactcagct ttgatcaaag aaaaaatctc tgttgccatc
     1441 gccttatccg ggacgagatc cacatagagg gcggcaatgt ccatatctgc cttgagcaaa
     1501 gacatctccg tgttatcgag catgctcttg aagaaagccc agccctgata catgtcttga
     1561 agaagtgcag gattttgtat cgattcaagc gcggtgccga gtccgaacca gccgggcaag
     1621 ttgaagcgac tctgcatcca tgaaaacacc caggggatgg cgcggatctt ggagacctca
     1681 ctgcttttcg ttctggccgc tggacgagat ccgatgtgca tacttttgat ctcgtctaaa
     1741 ggagtcgctg cctgccagaa atcaataaat gccggagttt catagaccag acctcgataa
     1801 gcccgaaagg cgactgacga catttcatcc atgaccgcgc gccaagagcc aggaatctct
     1861 gcccgagagt ttgcaggtga agaagcaacc agcactgcat ggacgatttg ctcgagatga
     1921 cgatgagcca actcaggatt tgagtatcgc gaggaaattg tttcaccttg ttcagtgaga
     1981 cggaaccggc cgttgatgct cccgggcggt tgggcacgaa tggcgcggtt ggctggacct
     2041 cctccgcgtg cgatcgttcc gccgcgaccg tgaaagatcg ttaatgtcac attatgtttt
     2101 tgggcaacct gcgtgatatt ctcctgaccc tgatacagcg cccagttcgc catcacatat
     2161 ccgccatctt tgttgctatc agaatagccg atcatgacca tctgttgatt cccgcatttg
     2221 gcaaggtgtt cacgatagag ctccagcgta aagaggcttt caagaatcga tgaggcgtcc
     2281 tgtaagtctt ctatcgtttc aaatagcggg acgatttgca gctcgtgatc acatccggcc
     2341 cagcgagcca gcaatagtac cgttaatacg tctgctgcag aacgacacat cgaaatcact
     2401 acaggaccca gtaactctgc tccataaaca tcccggacac gcttaatgag ttgaaacaat
     2461 gcccacgttt cggcagttgc cgccgtgatg cccgggtgtt ccgcgagatc gggtaaagag
     2521 gatgaaagca tttcggtgag aaacgagaga cgttccgcat ctggcatttc ggcgaagtta
     2581 ggagttatat tcaaggcgcg cagggtttct cccagtgctg cgttatagcg agaggactcc
     2641 tcgcggatat cgaggcgcat ggagtgcagt ccaaagatat ggagttgata gcggactttt
     2701 tcgatttcat cctgagcaat ggaagcaggc agtcctgctg aaataaaatc aagaggctca
     2761 agcaggtcgg gtaatttcat gcgggcttca tgaggcttgc gttccagcaa gcgcgcagtc
     2821 atatcatcct ttgaagcctc tgcaagatca gttgccagaa gggacagcac aaggcgatag
     2881 ggttcgtctg gataacgttt ctggatgtaa gcgacgtggt caggaagctt gccacgggat
     2941 tcaatccact tgatcaggtc gggttgaggc gacaaacggc gactgctgat gctcaggcga
     3001 cgcgccagat cctgcaatcg ttcgcggtgc tgctccactg ccagaccgcg atgaaggcgt
     3061 agcgtttcgg ccgtcactgt gtggtctaca ttcggattgc catcacgatc gccgcccatc
     3121 catgaggcca gccgaagcca gttggaaggc gcaacaacgt cggggtagtg agaagcgagt
     3181 gcgtcgtgaa gatcatcata gatctctgga agcgtatccc aaaaaacgga ctccacgaat
     3241 tgtaggccaa tccgaacctc atcttcgacc tggggccgtt ccgtgcggtg tacatcggta
     3301 agccacaaag aggcgacctc cgcatgaata cttgcgcgga tttccgtctc ctggtggtct
     3361 gaaatgggct tcctggaaat ttcatctaac aagcgtgtaa ggcgttgaag ttttgaaacg
     3421 actgtgcgcc gacgagcttc tgttggatgt gccgtgagga caagctcaat tgaaaggtcg
     3481 tcgagcatgt cttgcatttg cctgtaattt attccttctt ttttcagctg ggcaattgcg
     3541 tctaaaatcg actcgcccgg aggctcgttg gaaagaatcc tttcacgcaa attttcgacg
     3601 cggatttgtt cctccgccag gttgaccaat tcaaagtagg tagtaaatgc agcggagacc
     3661 ccccgcgcct gattgatatc cagggcttcc acatccgttt caagctgttt cgccgctttc
     3721 gcatctccat cacgacgatc tttcgaggcg aggcggattc gttcctcaat ggcgaaaagc
     3781 tccggtgatt cgagctcact gatcacctca ccaaggacat cgcccagcag atgaattatt
     3841 ctggaaattt cgatcttatt tggatctttc aactaacctc caatacgtcc gtcttccacc
     3901 gacaaaccct gcaacgagac ttagcagggc aatcacataa aaaattgcat tgaaataggc
     3961 gaaataacaa tcctcggagc aatccgccag gaaatattga gataacactt gtacaattat
     4021 ataatccagt acgatgccaa tgacgagaga aagtagcgta aatcctatcc cagaaagaat
     4081 gctcaaggta ataatcttag ttttgtcctg ctcattgttt tgaagattca taagcttcaa
     4141 ctaatccctt tcaaacggaa cgccgagtgc ggcaggaggc gaagcacgca tggaacggaa
     4201 agctctcgcc agaaatcgcc gcgtggattc gggaagggat gcaagagtcc gatccaccca
     4261 atctgcattg ccaatgttga cgagaagaag agtcagaatc atcaggggga acggcgcgac
     4321 ctgtaacacc tgtgaaggga caccgggcag gctgggttga agcacaagtc cgagccattg
     4381 caataaagcg aataagtatg caccaaacgc cgcacgcaaa ggattccacc cgccgaaaat
     4441 ggtgatcgcc aatgcaatcc agccgattcc atccaggcct gagattgtcc ctttccaccc
     4501 ggccttgacg ctcaatgaat aggcgggacc cgccagacca atgatcgcac caccaatcac
     4561 ggtatataca tatcgcataa gatttacatt cgcgccgcgc acataagatg cggcagggcg
     4621 ttcaccgatc ccctgcaaca tcaagccggg gtgggttcga aagatccaaa cccacgatac
     4681 gaaaatgata agataactta gatatgtgat cacgtcctgg ttaaataaca gtggaccaag
     4741 gacgggaatt tgacttagcc ccggaagcgg taacgccggc acgcgcggtc ccgcggcacc
     4801 catgatcgga ttgcccagaa agtaggaaag atcgcggcag acgagcgcca gcacgaagcc
     4861 caccgccact tgcgactgct tcaacgtaat cgacgaaaac gccacgatca gcgcgaccaa
     4921 ggcaccaatg gcggcaccgg tcagaaatcc gaggagcagg ctttcggtcc gaaccgaaac
     4981 gccgaaggca cccatcgcag aaagcaaaat cgtcccattc atcgataggt tgatcacacc
     5041 cgcgcgctca gagagagtct caccgatcac agcgaagaca atgggtgcag cggcaaacag
     5101 tacgctggca agtccaatga ttaaaagatc aaactccatg aggcctcacg aacgacggaa
     5161 aaattgttgg cgcacgccct ggacgatcaa aacaaaaagc acgagcatgc cttgcaatac
     5221 accagccaga gacgagtcaa gttggaggac aattggcaat tgaatactac cgatattcaa
     5281 cgcagcgaaa aacagagcaa cgggcacggc ccaaattgcc tgatagttta cgagcatggc
     5341 taccagcaga ccgagatagc catagccact tgaaatagag gggatcagac gatgatatac
     5401 ggcggttacc tgcattgcgc ccgctgctcc tgccaataga ccgcataaga tgaatgagac
     5461 catcagatac tgccaggttg ggatacccag catatatgca gctctgaaat ttttacctac
     5521 cgctttcaaa cgcagaccaa aatacgtccc ctgcaacaag aaatagacga caatgatggc
     5581 gataattgct aaccccaatg cccagggact taaccgcaag ccagtcatga caggcaacca
     5641 caaagtttcg tcaaacggtt ccgtaccgct cattgaaccg atgccttcgc gcttccaggg
     5701 tccgaagatc agccacaaag tcatcgccgt ggcgacgaaa ttcatgccca gtccaccgaa
     5761 gatttcgttc acaccaccaa atgttttcaa cgcgcctgca agcgctgccc acagtgcgcc
     5821 cgcaagcata cctgcgagaa tactgaatag gataatcaac ccgggcgtca gactgctatc
     5881 ctgcagggcg cgcaatgccc aggtcgtccc gattgcaccg agcgtgatct gaccctcgac
     5941 accgatattc cataaccctg ccgaaaatgt aattagtaag cctgcgctca cgagtagtaa
     6001 cggcacccaa ctcacaagca catctgtaaa ttttttgaac gagcctactg aacctatcac
     6061 aatattcttg taggcttcaa acggcggtgc acccgcgaga atcagaatta atgttgtgaa
     6121 aagcaaagcc aaaacaaatg cagcaatttg aaaaccaaaa tttgtccaac gggcgttttt
     6181 tgtcatagcc tcaatccttt tcatcccagc ccttaccccc gatcaactga cccaactgct
     6241 caacagaagt ttgtgcggcg tcttgtggag gcgaaacctt tccgctgaaa aataccagca
     6301 cgcggtcgct gtattgcagg atctcctcca ggtccgagga gatgaacaca attgcagccc
     6361 cctgcttgca gcgttctttc aatttgcccc acacataaat cgccgattcg atatcgagcc
     6421 cgcgtgtggg atgttcgatc agcgctatat ttaatgggtc actcaacaat gaaagcaggg
     6481 cgcgttgctg gtttccgccg gacaacgatt caatcggact gacaggtgtg cctttgatat
     6541 taaaagcctt gatcctctct tcggtcacat ggcgtccact ttcgcggtca atgaatacac
     6601 ccttttgttt tcccgccaac acgaaatgct ccgtgagtgt cagccctggc acgagtcctt
     6661 cctccaggcg cgcagcgggc aggaattcca ctccgttgct cttgaaagtg tgatatgtct
     6721 tgccagtgaa atctctgtca ttcaacttaa cttgtccgga tacgggtctg atcaacccgg
     6781 cacaggcccg caggaatgaa ctctgtccac taccttcgag tccagccagg ccgatcacct
     6841 cgcctgcccg aatttggaca ttcagatcct ggatgcgaag acggctatct tcaatgcata
     6901 catcattcaa actcaatacg tttttttcct gacccattgc ctggcgcccg ccgatcgtta
     6961 tattcttgcc aaacatcatc gccacaagtt tttccgtatt cgcgggcctt ttggcttccc
     7021 ctacaagttt tccctgacga aacacagcga ttgaatcact taatatttcc acgtcttcca
     7081 acttatgcga gacgaagagg atggtcatac cctggacccc caacttatta attagcgtaa
     7141 gaaaaagttt ttctttctgc gaagcgctaa tgcctgttgt tggttcatcc aggattaata
     7201 cccgcgcacc cagccacagc aggcgtaata tttccaactg ctgcctctcc cctacggtaa
     7261 gcaaatccac ataggagtca gg
//
LOCUS       DASTJP010000104         4288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_243780, whole genome shotgun sequence.
ACCESSION   DASTJP010000104 DASTJP010000000
VERSION     DASTJP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243780"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFQ23_03140"
     CDS             <1..301
                     /locus_tag="VFQ23_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET9905600.1"
                     /translation="SHPQYKIAKHQMKNGGGMISFLIKGGHEAAVKVVESTRIFTLAE
                     SLGGVESLIEVPAAMTHLSVAGSQLEVDAGLVRLSVGIENKNDLVEDIKQALEQA"
     gene            complement(346..1596)
                     /locus_tag="VFQ23_03145"
     CDS             complement(346..1596)
                     /locus_tag="VFQ23_03145"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9905601.1"
                     /translation="MKHESMSENLLGRIPLFADLPAEELDRLMSALDVVTLRSGDILF
                     REGDLGEHMYVMVKGELEINMASNTSNELILNILKEGEYLGEMSLIMPGGRRTAGARA
                     RGDVVLLSLSRKQFQDLLHRHPELANAMVKVLSQRLDNTNISTFRDLTEKNRQLQGAI
                     DELRAAQAQLIEKERMERELKVAADIQMSILPDVLPVHEHYDFGGRILPARQVGGDFY
                     DVFNLDENKIGVLIGDVADKGVPSAIFMARAHALIIAEADRAMTPGEVLMMANTHITR
                     FEKSRQFVTALYGVLDTKTGEFSYARAGHEPPLLLEPNGNVYRLPHQPGMALGLWEEI
                     LLDENTIHLPTGSVLMMYTDGMTDCRNPSGESFGLDRLKQTLAGLEHASAQASCDQLL
                     DTLMNYQDGSKQDDDVTLVAVHVK"
     gene            complement(1641..2936)
                     /locus_tag="VFQ23_03150"
     CDS             complement(1641..2936)
                     /locus_tag="VFQ23_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905602.1"
                     /translation="MKPIRVITLIWLAWALIVIAFQALATARISPEYPDRAQEWTEDS
                     TGPGYQRGHVFLLEPFMNNQVAWDSEYYLAIAIGGYDDPRSPHLTPQGVSTIVRDGQV
                     SQSGTNFAQSISLSYAFFPFYPLMIRIFALPLQIFGMNPIATASLAGVIVSALGTLLG
                     MLALYDLTRASLGEDGALRAAFYLIIFPTGFFLIQIYTEGLFVGLAFACLAMIQRKYW
                     LAAALLGVAATLTRAVGVTLLIPMAIAWLRTGEWVDLDLEWRQIIHQGIPVRPLARGL
                     LAFSPLLAFVIWKFSYLGLAFDYIETNHFGRGFLSLGYAFFAWSESFRSMLSGSNPQH
                     TAYYITEFLGLAIGAITCIAVMKSQPEIGWYSLAVFIISWGSGPAQGIHRYILAAPAV
                     FIALAQWGRNPVFDRAWTILSLLLMGLLSMLFAFDMWVA"
     gene            complement(2941..4020)
                     /locus_tag="VFQ23_03155"
     CDS             complement(2941..4020)
                     /locus_tag="VFQ23_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9905603.1"
                     /translation="MIFSRKRSSGRDASTLTLRLWDFTGTAMINLFMFLFIVAYLSPL
                     PFMLIASLTPSNQFLDANAPILPSERVKFNYEGENLIVYEVPTDQGVKQWALYKPSRQ
                     SSEFIDPANPEAGTIQWQGSWRTLKAVYQFKPTLDNYKEFLEVTNLPLLFKNTLLIAI
                     ISEIGVLVSSIAVAYGFSRFRIPGVRYIFFLLIATIMIPSNITLVPTYYLFSGHMLDW
                     IGSWLPLIVPHFFGSAILIFLLRQNFKSIPRDLDEAAMLDGASPLRILISVILPQSIP
                     VVTTVALLHFFYIWNETRFASLYLGVAPNLQPLSFGVQRSQSFFFTPEALMVGALVVM
                     IVPIIVLLISQRFFMQDMIVTQIEK"
     gene            complement(4010..>4288)
                     /locus_tag="VFQ23_03160"
     CDS             complement(4010..>4288)
                     /locus_tag="VFQ23_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905604.1"
                     /translation="LPIISPAIFFTLVINLTGAFGGAVLLDRGYILNFSLSPMEGYIN
                     NIMFSEADLGYASALAWVTFAVMMSITILLFRSARRWVYFPEEDSHDI"
BASE COUNT         1233 a         1059 c         1020 g          976 t
ORIGIN      
        1 gagtcacccc caatacaaaa ttgcgaaaca tcaaatgaaa aatggtggcg gtatgatctc
       61 gttcctcatc aaaggcgggc atgaagcagc ggtcaaggtc gtggaatcca caaggatttt
      121 tacacttgcc gaatcactcg gtggggtcga gtcgttgatt gaagtcccag cggcgatgac
      181 tcatctatca gttgcgggtt cacaactcga agtagatgca ggactggttc gattgtccgt
      241 tggtatcgag aataaaaatg atttggtgga agatatcaaa caggcactgg aacaagcgta
      301 aattgtaatg aaaagagaga ccgggttgtt gaacctggtc tctttttatt ttacatgaac
      361 ggcaacgagc gtcacatcat catcctgctt agagccgtcc tgataattca tcaacgtgtc
      421 cagtaattga tcacagctag cctgagcaga ggcgtgttcc aggcctgcga gtgtttgctt
      481 cagccgatcg agaccaaacg actccccgct ggggttcctg caatcggtca tgccgtccgt
      541 atacatcatt aacactgatc ccgttggtaa gtgaatggtg ttttcgtcca gaagaatttc
      601 ttcccacaaa ccaagcgcca taccaggttg atgcggtaag cgatagacat ttccgttggg
      661 ctccaacaga agaggtggct cgtggcccgc acgcgcataa gaaaactcgc cggtcttggt
      721 atccagaaca ccgtataagg cagtcacaaa ctgacgggat ttctcaaagc gcgtaatgtg
      781 agtatttgcc atcatcagca cttcacccgg cgtcatggct cggtctgctt ctgcaatgat
      841 aagggcgtga gcgcgtgcca taaagattgc agatggcaca cccttatccg cgacatcccc
      901 gatcagcaca ccgatcttgt tctcatccaa attaaagaca tcataaaagt caccgccaac
      961 ctgacgagct ggcaaaatac gtccaccgaa gtcatagtgc tcgtgaaccg gaaggacatc
     1021 aggcaaaatc gacatttgaa tgtctgccgc taccttgagt tccctctcca tgcgctcttt
     1081 ctcaatgagc tgtgcctggg ccgcccgcag ctcatcaatc gctccctgaa gttgacggtt
     1141 tttttcggta aggtctctaa aagtagagat attggtgtta tccaaacgct ggctaagcac
     1201 ctttaccatg gcatttgcca gttcgggatg ccggtgcaat aagtcctgga actgttttcg
     1261 actcaggcta agcaatacta catccccacg tgcccgggcg ccggccgtgc gcctgccgcc
     1321 aggcatgatg agactcatct cgcctaaata ttccccttct ttaagaatat tgaggatcaa
     1381 ttcattggac gtattcgatg ccatgttgat ttcaagttca cccttgacca tgacatacat
     1441 atgctcgccg agatcacctt cacggaaaag aatatcgccc gacctcaaag tgactacatc
     1501 cagcgcagac atgagtctat cgagttcctc agccggaagg tctgcgaaga gtggaatgcg
     1561 accaagcaaa ttctcagaca tcgattcgtg tttcatactg tcaatttacg cccactaatt
     1621 gtacactaag aatgcaatat ctacgcgacc cacatgtcga acgcaaacag catggaaagc
     1681 aatcccatca acaagagact caaaatcgtc caggcgcgat cgaacacagg gttcctcccc
     1741 cactgcgcta acgcgataaa tacggctggt gcggccagga tataacgatg aattccctgc
     1801 gcaggtccac tgccccagga aataatgaaa actgccaggc tataccatcc gatttcaggt
     1861 tggcttttca taactgcgat acacgtaatc gcgcctatcg ccagtcccaa aaattccgta
     1921 atgtagtacg cggtatgctg tggattactt ccgctcagca tagaacgaaa agactctgac
     1981 catgcaaaaa atgcatatcc taatgataag aagccgcgtc caaagtgatt tgtttcgatg
     2041 tagtcgaaag ccaacccaag atatgaaaac ttccaaatca caaatgccag caagggcgaa
     2101 aatgccagca accctcgcgc cagggggcgt accggaattc cttgatggat tatttgccgc
     2161 cattcaaggt ccaggtctac ccattcaccc gttcgaagcc aggcaattgc catgggaatg
     2221 agaagagtca caccaacagc gcgcgtcagt gttgcggcaa ctcccaataa cgccgcggcc
     2281 aaccagtact tgcgttggat catagccagg catgcaaagg caaggcccac aaacaggccc
     2341 tccgtataga tctgaatgag gaaaaagccg gttggaaaaa tgatcagata gaatgccgcc
     2401 cgcagcgctc catcttcacc gaggctggcg cgcgtcaaat cataaagcgc caacattcct
     2461 agcaaagtcc caagtgctga aacaatgacc cctgcaagcg aggctgtggc aattggattc
     2521 attccaaaga tctgcagagg aagcgcgaaa atgcgaatca tcagcggata aaatggaaaa
     2581 aaggcataac tcaaggaaat actctgggcg aaattcgtgc cagattggga tacctgacca
     2641 tcacgaacaa tagtggatac cccttgtgga gttaagtgtg gagacctggg atcatcatat
     2701 ccaccgatgg cgatcgcgag atagtactct gaatcccatg caacctgatt gttcatgaaa
     2761 ggttcgagta aaaatacgtg ccctctctga taacctggcc ctgtagagtc ttcggtccat
     2821 tcctgtgctc gatcggggta ttcaggtgat atgcgggccg tggccagggc ctggaaggca
     2881 atcacaatga gcgcccaggc caaccagatg agtgtaatca cgcgtattgg tttcatgaat
     2941 ttacttctca atttgagtaa cgatcatatc ctgcatgaag aatcgctgtg aaatgagcaa
     3001 aacgataatc ggcacgatca tcacgaccag cgcaccgacc atcagtgctt ctggtgtgaa
     3061 gaagaaagac tggctgcgct gaacaccaaa agaaaggggc tgaaggtttg gcgcaacgcc
     3121 aagatataaa ctggcaaacc gggtttcatt ccagatataa aagaagtgaa gtaaagccac
     3181 cgtggttacc acgggaatgg actgcgggag aatgaccgat atcaaaattc gaagagggct
     3241 ggcaccatcc agcatggctg cctcatccag atcgcggggg atgctcttga aattttggcg
     3301 caggaggaag atcaggattg cgcttccgaa gaaatgcgga acgatcaacg gtagccagct
     3361 acctatccaa tccaacatat gtccgctgaa cagatagtaa gttggtacaa gagtgatgtt
     3421 gctgggaatc atgatcgtgg caattaagag aaaaaagatg tatcgcacgc cagggatgcg
     3481 aaatcgtgaa aaaccatatg ccacagcaat tgacgacacc aacacgccga tctcactgat
     3541 gattgcaatt aacagcgtat tcttgaataa cagtggcaga ttggttactt ctaaaaattc
     3601 cttgtaatta tccagcgttg gcttgaattg gtacactgcc tttagcgttc gccaggaccc
     3661 ttgccattga atagtccctg cttctggatt cgctgggtca ataaactcac tggattgcct
     3721 cgatggtttg tataaagccc attgtttaac gccctgatcc gtagggactt cgtaaacgat
     3781 caggttttcg ccttcatagt tgaatttcac tcgttccgat ggcaatatcg gggcattggc
     3841 atccaggaac tggttactcg gtgtgagaga agcgatcaac atgaagggca gcggagaaag
     3901 ataggcaacg ataaacagga acataaacag gtttatcatt gcagtacctg taaaatccca
     3961 caatcgtaga gtcaatgtag aagcatcgcg accgctggat cgtttgcgac taaatatcat
     4021 ggctgtcctc ttcggggaaa tagacccatc gccttgctga gcgaaaaagc aaaatcgtta
     4081 tgctcatcat taccgcaaag gtaacccacg ccagtgcgct tgcataaccc aaatccgctt
     4141 cggaaaacat aatattgttg atataacctt ccatcggaga aaggctaaaa ttcaggatgt
     4201 agccacgatc caataacaca gcgccaccaa aagcaccggt caagttaatc actagtgtaa
     4261 aaaatatagc aggtgaaatt atgggtaa
//
LOCUS       DASTJP010000105        11023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_32355, whole genome shotgun sequence.
ACCESSION   DASTJP010000105 DASTJP010000000
VERSION     DASTJP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11023
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32355"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VFQ23_03165"
     CDS             <1..857
                     /locus_tag="VFQ23_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9905605.1"
                     /translation="GLISRSGQNVLGIRGVKYNRTVQSIYQVIAEIEKNNESAALCTV
                     VKSEGSTPRHTGSKMLVYPDGQFIGTVGGGELESRVIQAAIQSINSGEAQSLSYTMAD
                     PARGDPGVCGGTVEVFVEPILPPAMIVVIGGGHVGKAVVHLAKWLGFRVAVSDDRQQF
                     SNPEAVPGADAYYPVPMGKLTEHLNITRQSYIVITSRGSPIDAAGLPSLLDSEAAYIG
                     VIGSKRRWLTTVKALKEQGVSEEKIARVHSPMGIELNAETPEEIAVSILAEILMLRGK
                     GTGMLMND"
     gene            1010..2053
                     /locus_tag="VFQ23_03170"
     CDS             1010..2053
                     /locus_tag="VFQ23_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9905606.1"
                     /translation="MARIFDVIEYPSEMRDEIVHRFPESGIGDYRIGSQVIVRESQAA
                     VFFRDGNALDVFRAGRHTIATANIPKIIDWIGKAFNERTPFPAEVYFVSMKEFANEKW
                     GTPQPIIVRNPGMGLGVALLQGFGTFSFQVKDPQQFVTQVVGTQGAYRTSDIEERLKT
                     VLLSKLQDVLGETTAAKSVVDLIGLTEEIGASVRAKAQDDFEAIGLVLKSFYVGNLKP
                     STKSAQELRDMGMLDMATYTQLQAADAMRDAASNPSGGAGLTAGIGAGMGIGNLMQQA
                     TSGAMQQQQSGGGTKAAAGIPDVMTPAEAAAILKVSEEDVLAAINDKSLKAKKMGNAY
                     RISKAALEEFLSG"
     gene            complement(2245..3861)
                     /locus_tag="VFQ23_03175"
     CDS             complement(2245..3861)
                     /locus_tag="VFQ23_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9905607.1"
                     /translation="MKLVTVSQMQSIEKAADAGGLSFDQMMQNAGQGLAEIVIDLFAD
                     EDELKALGLVGSGNNGGDTLVALTALLGAGWKARAYLVKRKKDDLVKKFVEAGGEVLS
                     GEKDVEQLATSISEADVLLDGVLGTGIKLPLKKDVAETLAEVNDVLDGLSEHPLVIAV
                     DCPSGIDCDTGDVADETIPADITVTMAAVKHGMLKMPAFEYVGNLEVVDIGLPDDLPA
                     LKDLNSEVAEENLISLLLPERPIDSHKGTFGTALIAAGSVNYTGAVMLAAEAAYRVGT
                     GLVKLAVPAQVHAALAGQIPEATWIVLPQAMGVIASNAAEVLMKNIERATALLIGPGL
                     GMESTTKEFVENLLSGKTAPKKSTARIGFVHVDSEKQEESSSALPPMVVDADGLKLLA
                     QIKDWHTKLPASSILTPHPGEMAVLTNFSKEEIQEDRQEIARKYAREWGHVVILKGAF
                     TVIASPEGRVTVIPVASPALARAGTGDVLAGLVVGLRAQGLDAFDAAVVGAWIHAQAG
                     LYAADDLGTTASVLATDVLNSVSDVLSDLE"
     gene            4004..4456
                     /locus_tag="VFQ23_03180"
     CDS             4004..4456
                     /locus_tag="VFQ23_03180"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9905608.1"
                     /translation="MEVSNFEEIQPEFFRRVSQAVYCSVATVDRKNRPRSRIMHPVWD
                     GQVGWVISWPKSHKSKHLQGNPYVSMAYIQDTEHPVYVDCKAEWIDDVGEKHRIWDLH
                     RNTPPPLGFDPEPHYGTIHHQYYGILRFTPWRIELGNLNGAPIIWRSQ"
     gene            4672..6495
                     /locus_tag="VFQ23_03185"
     CDS             4672..6495
                     /locus_tag="VFQ23_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HET9905609.1"
                     /translation="MNTKVKDRPQEAVRESAPQVAEPQESTSRTPAPLQQYFNQTSIP
                     KDFINPNPKSVPDVQARVNNLKLRPTVPYAKLVASVNRIKPSLEPVEEMPEEIKSSVR
                     ALRPGLRRFHGTKLELSWFRIPGVVSPAADRFGYMSSPDVRRATKLPFNATTQALLGQ
                     LGELMGNVGREKNLESHNSSDEGVSTLPAGFTYFGQFVDHDITFDVSSSLDAETDAET
                     INNMRTPALDLDALYGRGPGLDPFLYVFPSSPLPSTAIKFAVGTNRNIGPGGPSDNGN
                     RDGMGVQTAFDVPRMPGTNTAVIGDPRNDENLIVVQFHHAMLRFHNAVVDLLLAAAFT
                     GDIFAEAKRIVTHHYQWAVVHDFLERICGTNAMSEAMRNVSPHRGQFRMPVEFAAAAY
                     RFGHSLIRDRYFVNFNFPQATLGQVFQFNRNPLLPVFSNWVVDFNAFFETNRATGTNN
                     LARKIDSFMANGLESLPGLQGMMAVLATRNLRRGLAFGLPSGQGMAEAFGIRPLSASE
                     LTLRLPKDEVALLKSNGGLLLSRTPLWYYILREAAVLNGGDRLGPVGARIVAETFVTM
                     LKRDTSSYLNVPGGFRPILPAVSRGDFTVADMLEFSGVTVP"
     gene            complement(6589..6999)
                     /locus_tag="VFQ23_03190"
     CDS             complement(6589..6999)
                     /locus_tag="VFQ23_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET9905610.1"
                     /translation="MNAVFPSEEWLKELETKLNSDQRYAEIARNWEGDLLFYIEPADN
                     LKEQLTIYLDLWHGKCRAVKYNPQPESFTSPAFALTSTYKDITAILSGTLNPLTAMMT
                     SKLRVQGSMGYMLRNIPTVLDFVRCAREVTAEII"
     gene            7168..7992
                     /locus_tag="VFQ23_03195"
     CDS             7168..7992
                     /locus_tag="VFQ23_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9905611.1"
                     /translation="MKKTYTLASLLVAATLALAACGGAATPTAMPVEPSGGTGGLPDL
                     GGREIRIAVENAYNPFNFIDEATGQAVGYDYDLFNEACKRLNCKPTFEQTSWDTIVAV
                     MGGTGQAEFDIAADGITITAERAAHVDFSMPYIALQQQLLVQEGESRFASADELNALG
                     DFKVGAQPGTTNYDLAVDLFGEDKIVAFDQYGLIVQALLNGDIDATVMDNVAGEGYIG
                     ANPNKLKLVGEPLTSEELGFVFPKGSDLLEPINLVLAEMQADGTLDTIYAKWFLTQ"
     gene            8083..8892
                     /locus_tag="VFQ23_03200"
     CDS             8083..8892
                     /locus_tag="VFQ23_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET9905612.1"
                     /translation="MSNQPSDTSNNSRQSRIAPSFERMAEWPWWLIVAILLGLFIVLQ
                     ITTNQRMSVIFGAIAAGIGVTIRVTLAAYVLAVLIGLVVGFARVSSNKIIFNIASFYV
                     EVVRGVPILVLLMYIAFVAVPLFAEMFGVRTRDFPNELRVILGLAIAYGAFESEIFRA
                     GIQSIEKGQMEAARALGMNYFQGMRYIILPQAIRRVLPALGNDFVAMLKDSSLVSVLG
                     VEDITQITKLYAASTFLFFQSYSILAFIYLSMTILLSRLVRALEVRLSAHR"
     gene            8903..9688
                     /locus_tag="VFQ23_03205"
     CDS             8903..9688
                     /locus_tag="VFQ23_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET9905613.1"
                     /translation="MNQNNQPTEAPIIKITNLHKVFHQLHVLKGINLDVHPQEVICVI
                     GRSGSGKSTMLRCINFLEEPSEGEIEIAGLKIPAGAKGKQHQELVHQARLLTGMVFQD
                     FNLFPHFTVLENVMRPSIIVKGTNPSVALEKAEMNLDRVGMLFKKDEYPSRISGGQKQ
                     RVAIARALAMEPQVMLFDEPTSALDPELIGEVLNVMKKLAKDGMTMIVVTHEMGFARE
                     VADRVVVMGDGEIIEDANPKDLFTHPKDPRTKALIDRYRTGGG"
     gene            9692..10453
                     /locus_tag="VFQ23_03210"
     CDS             9692..10453
                     /locus_tag="VFQ23_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HET9905614.1"
                     /translation="MLAITRDVSPRFNECEITHIERTPIDLEIARTQHHGYVQALKEL
                     RCAVLELPAEADLPDSVFVEDTALVLPEVALITRPGADSRKPEIESIIPVLRPYRELV
                     FIEAPGTVDGGDVLVLGKNIYVGKSTRSNASAVEQMNNLLGKYGYQAQHVDLHDCLHL
                     KTAATRVDDKTLLINRKWVDVENFEGFDLIDVDESEPFAGNCLPVNGSIIFPIEYPKT
                     GAKLEARGYKLKLVAAEELAKAEGQVTCCSLIISD"
     gene            complement(10557..10638)
                     /locus_tag="VFQ23_03215"
     tRNA            complement(10557..10638)
                     /locus_tag="VFQ23_03215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10602..10604),aa:Leu,seq:gag)
     gene            10737..>11023
                     /locus_tag="VFQ23_03220"
     CDS             10737..>11023
                     /locus_tag="VFQ23_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905615.1"
                     /translation="MTKIVVDAMGSDNYPAPDVEGALMAAREYGVDIILVGDSSKIQP
                     ILDSNKAANLPIRVINVPEMLTMHDKGEDLVMKARHKEAQNSMAVGLDLVK"
BASE COUNT         2763 a         2771 c         2804 g         2685 t
ORIGIN      
        1 caggattaat ttcacgttca gggcagaacg ttcttggaat acgcggggtg aaatataata
       61 gaaccgtgca atctatttat caggttatcg ctgagatcga aaaaaacaat gaatctgctg
      121 cgctttgtac ggtggtcaag agtgaaggat ccacgccgcg acatacaggc agtaagatgc
      181 ttgtctatcc cgatgggcag tttatcggca ctgttggggg tggtgaactg gagagtcgcg
      241 tcatccaggc tgccattcaa tccattaaca gcggcgaggc gcaaagtctt tcctatacga
      301 tggctgatcc tgcgcgcggt gacccaggcg tgtgcggcgg aactgtggag gtgtttgtgg
      361 aaccgatcct tcctccggcg atgattgttg ttattggtgg tgggcacgtt ggcaaggcgg
      421 tggtccatct ggcgaaatgg cttggattcc gcgtcgcagt aagcgatgac cgccagcaat
      481 tcagtaatcc cgaagcggtg cccggggcag acgcgtatta tccggttccg atggggaaat
      541 taacggaaca tctcaatatt acgcgccaaa gttatatagt gatcaccagt cgcggttccc
      601 cgattgatgc ggcaggcttg cccagtctcc tcgattcgga ggccgcctat atcggagtca
      661 tcggctcgaa acgcagatgg ctgacaaccg tcaaggcatt gaaagaacag ggtgtatccg
      721 aagaaaaaat cgcacgcgtg cattccccga tgggcatcga actcaatgca gagacccccg
      781 aggagatcgc cgtcagtatt ctggcagaga ttttgatgct gcgcggcaag ggaaccggca
      841 tgctgatgaa tgattaaata tagacccgaa aggtctttgc gaagcacccc cgaaatgtaa
      901 gcaggttgag acttttagcg tccaaatacg taattaaata agaaaaacgg ttcttgtaac
      961 gtgtcagtgc atggactata atcaaaaaag tctttactaa ggagcaaaca tggccagaat
     1021 ctttgatgtc atcgaatacc ccagcgagat gagagatgaa atcgtacacc gtttccccga
     1081 atcgggaatc ggtgattatc gtatcggctc acaggtgatt gttcgagagt cacaggcggc
     1141 agtattcttc cgggatggca acgcgttgga cgtctttcga gccggacgcc acaccatcgc
     1201 caccgccaac attcccaaga taatcgactg gatcggcaaa gcgttcaacg agcggacgcc
     1261 cttccccgcg gaagtctatt tcgtctcgat gaaggaattc gccaatgaga aatggggaac
     1321 acctcagccg atcatcgtcc gcaacccggg catgggatta ggtgtggctc tattgcaggg
     1381 attcggtaca ttttcgtttc aggtgaaaga cccccagcag tttgtgactc aggtcgtggg
     1441 cacgcaaggt gcctatcgca ccagcgatat tgaagaacga ctcaaaacag tcctactttc
     1501 caaactgcag gatgtgcttg gcgaaacaac ggctgcaaaa agtgttgtgg atctgatcgg
     1561 cctcaccgag gaaattggcg catccgtccg tgcgaaggca caggatgatt tcgaagccat
     1621 tgggctggtg cttaagagtt tctatgtggg caacctcaag ccctccacga aatctgctca
     1681 ggagctgcgc gatatgggta tgcttgatat ggcaacgtac acccagttac aagctgcgga
     1741 tgcgatgcgc gacgcggcgt ccaacccaag cggcggagca ggtctcacgg ccggaatcgg
     1801 agccgggatg ggcattggta atctaatgca gcaggctacg tcgggtgcaa tgcaacagca
     1861 acaatctggc ggcggaacga aagccgcggc tggcattcca gacgtgatga ctcccgccga
     1921 agctgcagct atcctcaaag tctcagagga agatgtgctg gctgcgatca atgacaaaag
     1981 cctaaaagcg aaaaagatgg ggaatgctta ccgcattagc aaagcagccc tggaagagtt
     2041 tttgtcagga taggttatac aggaaagtgg aaagaagaaa gaggaggctg agtaaattca
     2101 gcctcctctt tttgattaat aaattcgatg gtcgagtagc cgcaggcgta tcgagaccat
     2161 tagtaacaaa tttgcttttg attagtggtg gtttcgatac ggggcggaaa atcaccgccc
     2221 cactcaacca ccggattgtc atttttattc cagatcgctc aatacatcgg aaacagaatt
     2281 caacacatcg gttgccagca cggaagccgt ggtgccgaga tcatccgccg cgtataaacc
     2341 tgcctgcgcg tggatccacg cacccactac cgcagcatcg aaggcatcca atccctgagc
     2401 gcgtaaaccg acgacaagcc cagcgagaac atcaccggtc cccgcgcgcg ccagggcagg
     2461 agaggcaacc ggaatgacag tgacacgtcc ttctggtgag gcgatgacag taaacgcacc
     2521 tttgagtatc accacatgtc cccattccct tgcatacttt ctcgcgattt cctgacggtc
     2581 ttcctgaatt tcttcttttg agaaattagt cagtacagcc atctcgccgg ggtgaggagt
     2641 caagatcgaa gacgcaggca gttttgtgtg ccagtctttg atctgtgcca gtagtttcaa
     2701 tccatcggca tctaccacca ttggtggtaa cgcactgctg ctctcttcct gcttttcgct
     2761 gtctacatgt acgaagccaa tgcgtgccgt ggacttcttc ggcgcagttt ttcctgaaag
     2821 caggttttct acaaactctt tcgttgtaga ttccatccct aaccccggac cgatcagcag
     2881 ggcagtggcg cgctcgatgt tcttcatcag gacttctgcc gcgttcgaag cgatcacgcc
     2941 catggcttgg ggtaggacaa tccacgtcgc ttccgggatt tgacctgcca gcgccgcatg
     3001 gacctgggcg ggaaccgcca gtttgacaag cccggttcct acacgatatg ccgcttcggc
     3061 tgccaacata acggcaccgg tgtaattgac ggatcctgcg gcgatcaatg cagtaccaaa
     3121 cgtccctttg tgtgaatcaa tgggtcgttc aggtaacaat aacgaaataa ggttttcttc
     3181 tgcgacctct gaattcaaat ctttgagtgc aggtaagtca tcgggcaggc caatatcaac
     3241 gacttcaaga tttcccacat actcgaatgc gggcatcttc agcatcccat gtttaactgc
     3301 ggccatcgtg acggtgatat ccgccggtat cgtttcatct gccacgtcgc ctgtatcgca
     3361 gtcgatgcct gatgggcaat ccaccgcgat caccaatggg tgttcactca atccatccaa
     3421 aacatcattg acctcagcca gcgtttcagc gacatccttt ttcaggggca gcttgattcc
     3481 agttccaagg actccgtcca aaaggacatc tgcttcagag attgacgtcg ccaattgctc
     3541 aacatccttc tctccagaga gaacttcccc accagcttcg acgaacttct ttaccaggtc
     3601 atctttctta cgtttgacga gataggcacg cgccttccaa cccgcgccaa gcagcgcagt
     3661 taaagcaaca agcgtatcgc cgccgttatt ccctgaccct accaggccca gggccttcaa
     3721 ttcatcctca tcggcaaaca aatcgatgac gatttcagca agtccttgcc ctgcgttttg
     3781 catcatttgg tcaaaggata agccaccagc atccgctgct ttttcaatag attgcatctg
     3841 tgagacggta acaagtttca tttgtgagtt ctccaatagt gcgggaaatt gtacttcaaa
     3901 gcccaagtga gtcacagatt ctatgcttgg tctctaacga aaatcagata acagccaata
     3961 aggctgattt tcatcgatgt caggattcgt tataatccct gttatggaag tttcaaattt
     4021 cgaagagata caaccggaat tcttcagacg ggtgtctcag gcagtttatt gcagcgtcgc
     4081 tacggttgat cggaaaaatc gtccgaggtc ccgcattatg cacccggttt gggatgggca
     4141 ggtgggttgg gtaatttcgt ggcccaaatc acataaatca aagcacttac agggaaatcc
     4201 ttatgtatcg atggcttaca ttcaggacac ggaacatccg gtctatgtag attgcaaggc
     4261 agaatggatc gatgatgtgg gagaaaaaca tcgtatttgg gacctgcacc gaaatacacc
     4321 tccgcctctg ggctttgatc ccgagccaca ttatggcacc atccatcatc agtattatgg
     4381 gatactaaga tttacgccgt ggcgaattga gctggggaat ttaaacggtg cgccgatcat
     4441 ctggcggtca cagtagattt caaaaataca aatttcgaca tttctgcaac tctgacaaat
     4501 tcccaaacct agctgctttc accaaaatgg agggaatcct tcatgacgat agacgagcgt
     4561 atcgctacaa ttttcgtcgt tgaacaagaa aaacaataca gaatttcaat tcttttcaac
     4621 acgcaagtta tcgtaaaact ctacgttatg cacctatcca ggaggtagaa agtgaataca
     4681 aaagttaaag atcgaccgca agaggcggtc cgagaatcag cgccgcaagt cgcggaacct
     4741 caggaatcaa cgtccaggac tccggcgccg ttgcagcaat atttcaatca gacttcgatt
     4801 ccgaaagact ttatcaatcc caatccaaaa tcggtgccgg atgtgcaggc acgagtgaac
     4861 aacctcaaac tgaggccaac cgttccatat gcgaagttgg tcgccagcgt gaatcgcatc
     4921 aaaccgtccc ttgagcccgt cgaagaaatg cctgaagaga tcaaatcgtc ggtgcgcgct
     4981 cttcgaccag gtctgagacg tttccatggc accaagctgg agctttcctg gttccgcatc
     5041 ccgggggtgg tttcgcctgc tgcagatcga tttggatata tgtcatcgcc ggacgtccgg
     5101 cgggcgacaa agttaccgtt taacgccact actcaggcgc tccttggcca actgggtgaa
     5161 ttgatgggaa atgtcggtcg cgagaaaaat ctcgagtcgc ataactcatc agacgagggt
     5221 gtatcaaccc ttcctgcagg tttcacttac ttcggtcagt ttgtggatca cgatattaca
     5281 tttgacgttt catcctcgct ggatgcggaa accgacgcgg aaacaatcaa caatatgagg
     5341 acgcccgctc tcgacctcga tgccttgtac gggcgcggtc cgggtttaga tccgttcctt
     5401 tatgtttttc cttcctcgcc gcttccatcc actgcaatta agttcgccgt gggcacaaac
     5461 cgaaacattg ggcccggtgg gcctagcgac aatggcaacc gggatgggat gggagtacaa
     5521 accgccttcg atgtcccccg aatgccaggc accaacaccg cggtaattgg cgatccgcgc
     5581 aatgacgaaa acctgatcgt cgtgcaattc catcatgcaa tgctccgctt tcataacgca
     5641 gtagtcgatt tgttgttggc ggccgcgttt actggggata tcttcgcgga ggccaaaagg
     5701 attgtgacgc accactatca atgggcggtc gttcatgact tcttggaaag aatttgtggt
     5761 accaatgcaa tgagcgaggc aatgcggaat gtcagtccgc atcgcggcca attccgtatg
     5821 cctgtcgaat ttgcagcggc tgcataccgg ttcggtcaca gtctgattcg cgatcggtat
     5881 tttgtgaatt tcaatttccc gcaggcgacg cttggccagg tattccagtt caaccgcaac
     5941 cccttgctgc cggtcttctc gaattgggta gtggacttca atgcgttctt cgaaaccaac
     6001 cgcgctaccg gaaccaataa tttggcacgg aaaattgata gcttcatggc aaatggactt
     6061 gaaagtcttc cgggtttgca gggaatgatg gcagttctgg caacacgcaa cctgcgtcga
     6121 gggttggcct tcgggctacc aagtggacaa ggcatggcgg aagcgtttgg catcagacca
     6181 ttgagtgcct cagagttgac cttgcgattg cctaaagatg aagtggcttt actgaaatcc
     6241 aatggcggtt tgttgctgag tagaactccc ctgtggtatt acatcctgcg cgaagcagca
     6301 gtcctcaacg gtggcgatcg attgggtcca gtgggcgcca ggatcgttgc agaaacgttt
     6361 gtcacgatgc taaagcgaga cacgtcttcc tatctaaatg tacccggcgg tttcaggcct
     6421 attcttccag cagtatcaag gggagatttt actgtggcgg atatgttgga gttctctgga
     6481 gtgaccgttc cgtagagcaa gcgcaacgaa tattctttgc gatgcaaaat cgggctgaca
     6541 gtttgtcagc ccgattttat tttctataca ccgctgagac tccccttgct atatgatctc
     6601 agccgtcact tcgcgtgcgc aacgaacaaa atccaacacg gtgggtatgt tacgcagcat
     6661 atatcccatg ctcccctgca ccctaagttt cgaggtcatc atcgctgtca aaggatttaa
     6721 ggtaccagac aaaattgcag tgatatcttt ataggtagat gtgagagcaa aggcgggact
     6781 cgtaaaagat tcaggctgag ggttgtattt tacagctcgg cattttccgt gccagaggtc
     6841 taaataaatc gtaagttgct ctttcaaatt gtcagcaggc tcaatgtaaa acaataaatc
     6901 cccttcccag ttcctggcaa tctcagcata tcgctgatcg gaattcagct tcgtctccaa
     6961 ctcctttaac cattcctcgc tgggaaaaac agcattcatc tcgatctcca taaacaggat
     7021 aaattattgt accatttgaa attaagcatc acgaatcact tgcacaattg ctaaaaatag
     7081 tctatgctta ggtcacgaga tacgcctgtg aatctcgtgc aatctcgtga ttgcttaaaa
     7141 tccattctat tctcttggag gagaaatatg aaaaaaacgt atactttggc cagtctcctt
     7201 gtggctgcta cgcttgctct tgcagcctgt ggaggggctg ccactccaac cgctatgccg
     7261 gttgaaccaa gtggcgggac aggcggattg cccgatctgg gcggccgtga gattcggatt
     7321 gcggtggaga acgcgtacaa tccgttcaac ttcattgatg aggccactgg tcaagctgtg
     7381 ggatatgact atgatctctt taatgaagcc tgcaagcgtc tgaactgcaa acccaccttt
     7441 gagcaaacaa gctgggacac cattgtggct gtgatgggtg gcacgggtca ggccgagttt
     7501 gatatcgccg cggatggcat caccattacc gccgaacgcg ccgcgcacgt tgatttctcg
     7561 atgccgtata ttgcacttca gcaacagttg ctcgttcagg aaggcgaatc acgcttcgcc
     7621 agcgcggatg agctgaatgc cctgggagac tttaaagttg gagcgcaacc tggcacaacg
     7681 aattacgatc tggctgtgga tctgttcggt gaagataaaa tcgtcgcctt tgaccagtat
     7741 gggttgattg ttcaggctct gctcaatggc gacattgatg cgactgtaat ggacaatgtc
     7801 gcgggtgaag gctatatcgg cgcaaacccc aataagttaa agttggtcgg tgagccgcta
     7861 accagtgaag aactcgggtt cgtattcccg aaaggcagtg atttgctgga gccgatcaat
     7921 ctggttctcg ctgaaatgca agccgatggc acgcttgaca caatctatgc caagtggttc
     7981 ctcacacaat aaataatcgg taatcacaag cctgccagaa atctctcggc aggcttgtgt
     8041 tctatttatt tccatccgtc ttttctgcca aggcgacctg tcatgtccaa ccaaccatcc
     8101 gatacaagca acaactcgcg ccagtcgcgt attgcacctt ccttcgagcg tatggcagaa
     8161 tggccctggt ggctgatcgt cgccattctc ctaggcctgt ttatcgttct ccagatcacc
     8221 accaaccaac gaatgtccgt cattttcggt gcaattgccg ccgggatcgg cgtcacaatt
     8281 cgggtcacac tagcggccta cgttttggca gtattgatcg ggcttgtggt cgggtttgcg
     8341 cgtgtctcaa gcaacaagat cattttcaat atcgcgtcct tttatgtaga agtggtgaga
     8401 ggcgtcccca ttctggtctt gttgatgtat atcgcgtttg tggcggtgcc cctttttgct
     8461 gaaatgtttg gcgttcgcac gcgcgatttt ccgaatgagt tgcgcgtcat cctggggttg
     8521 gcgattgcgt atggagcgtt cgagtccgag atttttagag caggcatcca gtcaattgaa
     8581 aagggtcaga tggaagcggc gcgtgcgctg ggtatgaatt attttcaggg aatgcgctat
     8641 atcatcctgc ctcaggcaat tcgccgggtg ctgccggccc tcggcaatga tttcgtggcc
     8701 atgctcaagg attcatccct tgtctcagtg cttggcgttg aagacatcac gcagattacc
     8761 aagctctacg ctgccagcac ctttttgttt ttccaatcgt acagtattct cgcttttatt
     8821 tatctttcca tgaccatcct gctgtcacgt ctggtacgcg cgcttgaagt cagactttca
     8881 gcacaccgtt aatcaaacgc ccatgaacca aaacaatcaa cctacagaag ccccgatcat
     8941 caagatcacc aacctgcaca aggtattcca tcaattgcac gtgttaaaag gaattaacct
     9001 ggatgtccat ccgcaggagg tgatttgtgt aatcggacga agcggatcgg gcaaaagcac
     9061 aatgctgcga tgtatcaatt ttcttgagga accctcagaa ggtgagatcg agatcgctgg
     9121 tctcaaaatt ccagcgggtg ccaaaggcaa gcagcatcag gaacttgtcc accaggcgcg
     9181 cctcctcaca ggcatggtat ttcaagactt caaccttttc cctcatttca ctgtgctgga
     9241 aaatgtaatg cggccctcga tcattgtaaa aggcaccaac ccatccgtcg cgcttgaaaa
     9301 ggcagaaatg aacctggatc gcgtcggtat gcttttcaag aaggacgagt atccgagcag
     9361 aatttcaggc ggacaaaaac aaagagtcgc catcgcgcgc gcgctggcca tggaaccgca
     9421 agtaatgctc tttgatgaac ccacgtcagc gctcgacccg gaactgattg gtgaagtact
     9481 caatgtgatg aagaagttag caaaagacgg gatgaccatg atcgtagtta cgcatgaaat
     9541 gggattcgca cgagaagttg cagatcgcgt cgttgtgatg ggcgacggtg aaatcattga
     9601 ggacgccaat ccgaaagact tgttcaccca ccccaaagat ccacgcacga aagcccttat
     9661 agaccgctac cgtacaggag gaggttagtt gatgcttgcc atcacccgtg atgtaagtcc
     9721 ccgttttaat gagtgcgaga tcacgcatat tgaacgcact ccaattgacc tggagattgc
     9781 gcgaactcaa catcatggtt acgttcaggc gctcaaggaa ctcagatgtg ctgttttaga
     9841 gttgcccgcc gaagcagatt tacccgactc ggtatttgtg gaggatacag cccttgtcct
     9901 gccggaagtg gccctcataa ctcgaccggg ggctgattca cgaaaacctg aaattgaatc
     9961 aattatccca gtgctgcgac cctatcgcga actcgttttt atcgaggcgc ccggaactgt
    10021 ggatggtggc gatgtactgg tgctcggtaa aaatatttac gttggcaagt ccacccgtag
    10081 taacgcatct gctgtggaac agatgaataa tctgcttggc aaatatggtt accaggccca
    10141 gcatgtggac ctgcatgatt gtttgcatct taaaaccgca gctacgcgcg tggatgacaa
    10201 aaccttgttg atcaatcgca aatgggtgga tgtggaaaac ttcgaaggct ttgatctgat
    10261 cgatgtggat gaatcagaac cctttgccgg aaactgtctg ccagttaacg gctcgatcat
    10321 cttcccgatt gaatatccaa aaacaggcgc aaaattggaa gcgcgcggat acaaactcaa
    10381 actggttgcc gcagaggaac tcgccaaagc agaaggccag gtgacctgct gcagcttgat
    10441 tatttcggat taatgaagag attgcttggg gctctcgcct tcccaacgac ctgtgctgaa
    10501 tttaaacgat acagggttcg cagtagcgaa ccctgtatac ttattattgc aatttgtgcg
    10561 ctggagagga cttgaacctc cacgccttgc ggcacatggc cctcaaccat gcctgtctgc
    10621 caattccagc accagcgctt gagcacggcg tattataatc tgcttgcttc gcttgtcaag
    10681 caatctggct ccatcggctc tgagaaccga tggaggcata atatcatgga gacttcatga
    10741 ccaaaatcgt tgtggacgcg atgggaagtg acaactatcc tgctccagat gtggaaggcg
    10801 cgctgatggc agcacgcgaa tacggtgtgg atattattct tgttggcgac tcttcaaaga
    10861 ttcaaccgat acttgattcc aataaagcag caaatctacc catccgcgtc ataaacgtac
    10921 ctgagatgct gaccatgcac gataagggtg aagacctggt gatgaaggcg cgtcataagg
    10981 aagcgcagaa ctccatggcg gttgggctcg atctggtcaa aaa
//
LOCUS       DASTJP010000106        11123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_746315, whole genome shotgun sequence.
ACCESSION   DASTJP010000106 DASTJP010000000
VERSION     DASTJP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746315"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFQ23_03225"
     CDS             <1..745
                     /locus_tag="VFQ23_03225"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9905616.1"
                     /translation="QLIKVARGDLFVSRVGQDLRDSARQTYDEAKANLDETQLTYNDA
                     LSTDGAKEVLQARADLSIAQERYYTAMDYTLSLQIGESSTSVTAAQKGLEQAQSVVDQ
                     AKTAIGQAEANLALIDTQIAKLTIYTPMDGVILTRSVEPGEFVQPGAAALTMANLSEL
                     TITVYVPETRLNEIRLGQQASVTIDVASGQSPVFDAEVIHIADQAEFTPRNVQTVEGR
                     SSTVYAVKLKVTDTEGKLKIGMPADVVFE"
     gene            760..1722
                     /locus_tag="VFQ23_03230"
     CDS             760..1722
                     /locus_tag="VFQ23_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9905617.1"
                     /translation="MNKLLVSAFNISKSFGDTRAVDNVSLQIKAGEIYGLVGSDGAGK
                     TTTMRLLVGALKNDSGEINICGYDINTQTEQARAQFGYLSQRFSLYEDLTVLENIRFF
                     AEARGLKSNEWLPRCNGILEFVGLEKFKDRRAGQLSGGMKQKLGLASALVTRPRVLLL
                     DEPTTGVDPVTRQDFWQLVIKLVSPSPTGREDRGEGGVSVLISTPYMDEASRCHRVGF
                     MKNGKIIAEDTPSNLRARLKDRILELRGSPLNLLRHISHKDEDVEDVQAFGDRLHLRV
                     RQNKAREVLYRLQSRIGNEGGHVDELRAVNPVLEDVFIALSESK"
     gene            1719..2627
                     /locus_tag="VFQ23_03235"
     CDS             1719..2627
                     /locus_tag="VFQ23_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9905618.1"
                     /translation="MNEAVIQVENLTRRFGEFVAVDHVNFEVHRGEVVGYLGPNGSGK
                     TTTIRMLLGLLEPSDGKATVLGFDAFRQSEEVRARAGYMSQKFAIYDDLTVLENLMFY
                     GGVYGIRDKARIKQTLDLVGLASHDSTLTSSLSAGWRQRLALGIALVHEPKLLFLDEP
                     TSGVDPTARRAFWDLIYELAENGVTILVTTHYMDEAEYCERVGIMRDGKLLAMDTPSN
                     LKKDFISGDVWEVYAEPLQDGLEVLPGLDGIIHVGLAGDHLRTITERGKQRDELLIDL
                     KNRSVNVRDIILGEPTLEDVFISLAR"
     gene            2701..3957
                     /locus_tag="VFQ23_03240"
     CDS             2701..3957
                     /locus_tag="VFQ23_03240"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET9905619.1"
                     /translation="MPSMSDVLGMIMGGGRGARLFPLTTMRSKPAVPIAGKYRLIDIP
                     ISNCINSGIFRVSILTQFNSVSLHRHISQSYNFDAFHTGWVDILAAEQTPETETWYQG
                     TADAVRKQMFEIQATGAKYVLILAGDHLYRMDYRSMAEYHWENNADITVAVQPVARSE
                     ASRFGILKKEADGRISAFTEKPKDPEIQKQFVSREHDEERPFLGSMGIYLFNTKILLD
                     MLKTYTDMDDFGGDIIPEAIKSHQVFGFDFDGYWEDIGTIRSFYETNLRLTDPNPPFN
                     FYDPKAPIYTHARFLPGSIIEDSQLKDVLVAEGCRIRKAEITKSIIGIRSQVAEGAVI
                     KDSIMMGADYYTPKHGSLPLGVGANCHIESAILDKNVQIGEGVVILPFPRDSGDIDHP
                     LYHVRDGIVVIAKDTEVPAGTRIAPE"
     gene            4066..4935
                     /locus_tag="VFQ23_03245"
     CDS             4066..4935
                     /locus_tag="VFQ23_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905620.1"
                     /translation="MELYSVILIVVQLIFLEGILSIDNAAVLGAMVAPLPDDQAIPWP
                     AWLQGIGERLDHLLGNQRLAALRVGLLGAYVGRGAMLFITSFLIHNEWIRLVGAAYLI
                     RLAFDELGDTTPNEVTEQEKKDAIKGVSFWSVVLTVELMDLVFSIDNVIAAVSLSDEI
                     WVVMLGVGIGILAMRFAAGLFSYAVQREPVLKIAAYILLLNIGIQLILEQLWKVEISD
                     ITRFAVSMGIILASLAYAHSAFLQRFQFIFSWLSLGMGLLNELINWIFLPVKALFQWI
                     ANGFKPVKLAEIE"
     gene            5179..8073
                     /locus_tag="VFQ23_03250"
     CDS             5179..8073
                     /locus_tag="VFQ23_03250"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET9905621.1"
                     /translation="MPHDGENTKQEWHALTAEEVLNHLKVEDNGLNSEEARKRLDHYG
                     PNQLTEAPRPTFLQMLWEQLNNFVVILLIVASVISAVLGDYVEAAAIMAIVVLNAILG
                     IVQERRAEEALAALKKLAAPDAQVLRDGRRQSIPGYELVPGDIVFLEAGNFVPADIRL
                     LEAINLRIEEASLTGESLAVQKNAASVLDKNLPLGDRKNTAFMGTVVSYGRGRGVVTS
                     TGMHTQLGLIATMLQNVETEETPLQRRLDQLGRSLSIVALFLVIIVFIVALINYTDTT
                     QLFSQPLEYLREFGADITEVFIIAISLAIAAVPEGLPAVVTISLALGMREMIKRHALI
                     RKLSSVETLGSATVICSDKTGTLTQNEMTVTELWVDGQFINITGTGYAPKGDFLVDGK
                     QVEIKNYPAALTALWLGLLNNDAEIEITGESEQNKTYRIVGDPTEGALVVAAAKAGAI
                     HVEIKEAYPRENEAPFDSERKRMITIHDVRSPRAHDPSPFDEQLKDWDVIAVKGAPDV
                     VLELCTQYQGRDDSQQPLDDVGRERILAANDRMTKEALRVLGVAYRIARDVPDNPEHI
                     KTEELEQDLVFVGLLGMIDPPRAQVKPALAHARDAGIRTIMITGDYPNTARAIAESIG
                     MLQQGRQVMTGAQLDAISDQELRNVIKDTAVFARVSPEHKMRIVDALQVNDEIVAMTG
                     DGVNDAPAIKRADIGIAMGITGTDVAKETADMVLTDDNYASIVSAVEQGRIIYSNIRK
                     FVFFLLSSNLAEIMIIFLATLAGLPAPLTAIQLLWLNLITDGAPALALAVEKGDPDIM
                     EQKPRAKSEPIVNRSMTIGLVVQTIAQTGAVLTAFALGLLWHLEAGATVVGNPIAYLI
                     AHDWRGIDVQTAETMAFVTLSLAELFRAYTVRSERASIFQIGIFSNRYMQYAVGLSIV
                     LLLLVVNVPFLQPIFNTHSPNLREWGVVLGLSIIPAVAEEITKFFLRRQS"
     gene            complement(8141..8758)
                     /locus_tag="VFQ23_03255"
     CDS             complement(8141..8758)
                     /locus_tag="VFQ23_03255"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET9905622.1"
                     /translation="MIVNPNLNYEKRLWSCYQYVAGLDEAGRGALAGPVAVGAVILPK
                     DKTRLARTLAGVRDSKQMTPLERDALAPRIQEIAVAWSVAFASAEEIDTQGIVPATRL
                     AALRALQNLSISPEYLLTDFRLELPQVELGQTSLVKGDACCLSIAAASVLAKTARDAY
                     MNQLDSEYPNYGLGKHKGYGTQAHRLAMKQWGYSSIHRKSFSLKS"
     gene            complement(8742..9470)
                     /locus_tag="VFQ23_03260"
     CDS             complement(8742..9470)
                     /locus_tag="VFQ23_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HET9905623.1"
                     /translation="MSSFLSIIIPAYNEENRLPNSLEQVFRFLEKQSYTAEVIVVENG
                     STDKTLEVASNFAKKHKNLRVFHSEQRGKGSAVLRGVRESQGEYVFICDADLSMPIEE
                     ITKFLPPALTDFDIAIGSREAPGSVRYNEPYYRHLTGRVFNTLIRLMVLPGLQDTQCG
                     FKCMRAVVAHDIFRLQTITGWAFDVELLFIAHRRGYRIVEIPINWNFNADSKVRVLRD
                     AWRMFVDLLIIRWNAVRGLYDRKP"
     gene            complement(9478..10434)
                     /locus_tag="VFQ23_03265"
     CDS             complement(9478..10434)
                     /locus_tag="VFQ23_03265"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HET9905624.1"
                     /translation="MKIIDKTPLLDEKGELGFSQRIQGMFQFGFNWPNELQTQKAIIT
                     FFDRQLEKGYTLIRNIPLGQSGIIVPMILLGPTGIHVIQIANVRGRYEVRGDMWNVEA
                     GEKYKPAPVNLVQQTIRMANAVRVFIERQGVRLPAAPEPVLIAGDPGLHISSVQPALK
                     AMMIDGIKSFVSSLAIGTAILAPEQVFDYTERLINPRPSKKDSPASSAVITAPRNEWE
                     QAPYQPQDVSRARAIFDASEDAKPFNPADFEFEMMEEEPYTDIPPATLPQADSESPSQ
                     EPVRKRSKILGMTPAQLAILAGLALALVCILVAFGYIIFTRT"
     gene            complement(10474..10776)
                     /locus_tag="VFQ23_03270"
     CDS             complement(10474..10776)
                     /locus_tag="VFQ23_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905625.1"
                     /translation="MAKKIDGVITAARYENGQIVAVRAYERRGATFSDHVFLDRKELL
                     ERIKNGKKFVVGERKELLASTFEMGKPVEVLHHHGKEIIATRNEADRDELEQAPVF"
BASE COUNT         2715 a         2713 c         2890 g         2805 t
ORIGIN      
        1 tcaattaatc aaggtagcca gaggtgatct ttttgtcagc cgcgtcgggc aggatctgcg
       61 cgactctgcc aggcagactt acgatgaggc taaggcgaat cttgacgaaa cccaactaac
      121 gtataacgac gccttatcca ccgatggagc aaaagaggta ttacaggccc gtgccgatct
      181 cagcattgcc caggagcgtt actatacagc catggactat accctctccc ttcagattgg
      241 ggaatcttcc acatcagtca ccgccgctca gaaggggctc gaacaggctc aatctgtggt
      301 agatcaagcc aaaacggcga tcggacaggc agaagcgaac ctggcgttga tcgatactca
      361 aatcgcaaaa ctaacaatct atactcctat ggatggagtt attctcacgc gcagtgtcga
      421 accgggtgaa ttcgtccagc cgggcgccgc agcactgacg atggcgaacc tgagtgagct
      481 gacgattaca gtgtacgtac ccgagacacg tctgaacgaa atcagactcg ggcaacaggc
      541 ttctgtcacg attgatgttg cgtctggtca aagccccgtc tttgatgccg aggtgattca
      601 tatcgcagat caggctgaat tcacgccgcg caacgtccaa actgtggaag gacggagttc
      661 cacggtctat gcggtcaaac taaaagtgac agatactgaa ggcaaattaa aaatcggcat
      721 gccggcagac gtcgtttttg agtaaccatc gaaggcccaa tgaataaact tctggtttct
      781 gctttcaaca taagtaaatc ctttggcgat acccgcgcgg tagataatgt ttctctgcag
      841 atcaaagcag gcgagatcta tggactcgtt ggctcggatg gggcgggaaa gacaacaaca
      901 atgcgcttgc tggttggtgc gctcaaaaac gattcaggcg agatcaatat ctgtggctac
      961 gatatcaaca cacagaccga acaagcccgc gcccaattcg gttatctatc gcagcgcttc
     1021 tccttgtatg aagatttgac agtccttgaa aatatccgtt tctttgccga agcacgcggc
     1081 ctgaaatcaa atgagtggct ccctcgctgt aatgggattc tcgagtttgt cggcctcgaa
     1141 aagttcaaag ataggcgcgc tggccaactc tcgggtggga tgaaacagaa actgggcttg
     1201 gcgtctgcac tggttactcg tccgcgcgtt ttattacttg acgagcccac aacaggcgtt
     1261 gacccagtca cccgtcagga tttttggcag ttggttataa agttagtctc cccctctccc
     1321 actgggagag aggatagggg tgagggcgga gtatctgtcc tcatctccac cccctacatg
     1381 gacgaagcct cgcgttgtca ccgtgttggc tttatgaaga acgggaaaat cattgccgag
     1441 gatactcctt ccaatttacg cgctcggctc aaagatcgca ttcttgagtt gcgtggttca
     1501 cctttgaatc tacttcgcca tatttcacac aaggatgaag acgtggaaga cgtccaggcc
     1561 ttcggtgatc gattgcattt acgcgttcgg cagaacaagg cacgagaggt cctttaccga
     1621 ttgcagtcga gaatcggcaa cgaaggtggc catgtcgatg aactgcgcgc tgtcaatccg
     1681 gtcctcgaag atgtgttcat tgcattatcg gagtccaaat gaacgaggca gtcattcagg
     1741 ttgaaaatct cacgcgtcgg ttcggtgagt ttgtcgcagt ggatcatgtc aattttgagg
     1801 tgcatagggg cgaagttgtc ggatatttgg gacccaatgg ttcgggcaaa acgaccacca
     1861 ttcggatgtt gttaggtttg cttgagccca gtgacggcaa agccacggtt ttaggtttcg
     1921 atgcattcag gcaaagcgaa gaagtgcgcg ctcgggcagg atacatgtca caaaagttcg
     1981 cgatttacga tgacctgact gtgcttgaaa atctgatgtt ttacggcggc gtgtatggga
     2041 ttcgtgacaa ggcccgcatc aaacaaaccc ttgacctggt tgggctcgct agtcatgact
     2101 cgaccctgac gagttcgctc tccgcaggct ggcgtcagag actcgccttg ggtatcgcgc
     2161 tggtgcacga accgaaacta ctcttcctcg atgaacccac ctcaggcgtt gacccgactg
     2221 cccgccgcgc cttctgggat ctgatctatg aactcgccga aaatggcgtg acgatactgg
     2281 ttaccactca ttacatggac gaagccgagt actgtgagcg tgtcggtatc atgcgcgatg
     2341 gaaaacttct agcaatggat acgccgtcga atttgaaaaa ggacttcatc tctggtgatg
     2401 tttgggaagt atatgcggaa cctttacagg atggtttgga agtattaccc ggtctggatg
     2461 gaataatcca tgttggcctg gcaggtgatc acttgaggac aattaccgaa agaggaaagc
     2521 agagagatga attgttgatc gatttgaaaa atagaagtgt gaatgtacgc gacatcattc
     2581 tcggcgagcc aaccctggaa gatgtgttta tttcgctggc gagataatca atatcttttc
     2641 acttttccca attttaagag ataatagaac ccatcacatc atttcaatct ggaggctgct
     2701 atgccatcaa tgagcgatgt cttgggtatg attatgggtg gaggtcgggg cgcacgattg
     2761 tttccgttga ccacaatgcg ttcgaaacca gctgttccaa tcgccggcaa atatcgcctg
     2821 atcgatattc ccatcagcaa ttgcattaat tcagggattt ttcgggtctc gatattgact
     2881 cagttcaatt cggtttcact acaccgtcac atttcccagt cgtacaactt cgatgccttc
     2941 catacgggtt gggtggatat cctggctgcc gagcagacgc ccgaaactga aacctggtat
     3001 cagggcacgg cagatgccgt acgaaaacag atgtttgaaa tccaggcaac tggcgcgaaa
     3061 tatgtattga tcctcgcggg agatcatctt tatcgcatgg actatcgctc catggcggaa
     3121 tatcattggg agaacaatgc tgatataacc gtggcagtcc agcccgttgc gagaagcgaa
     3181 gcctctcgct ttggcattct caaaaaagaa gccgatggtc gaatatctgc ttttactgag
     3241 aagcccaaag atcctgaaat ccaaaaacaa tttgtcagcc gtgagcatga tgaggaaaga
     3301 ccgtttttag gctccatggg gatttatttg ttcaatacaa aaatattact cgatatgctt
     3361 aaaacatata cagatatgga tgattttggc ggggacatca tccctgaggc gatcaaatct
     3421 catcaggttt ttggctttga tttcgatggc tattgggagg atatcgggac gatccgttcg
     3481 ttctatgaaa ctaatttaag acttactgat cccaacccac cgttcaattt ttatgatcca
     3541 aaggcgccca tttatactca tgcgcgtttt ttgcctggtt cgatcattga ggatagccag
     3601 ttgaaggatg ttttggttgc agaaggatgc cgaatccgca aggcggagat cacgaaatcc
     3661 attattggta ttcgcagtca ggtggcggaa ggggcagtca tcaaagacag tattatgatg
     3721 ggcgcagatt actatacgcc caaacacggt agtcttcccc tgggcgttgg ggcaaattgt
     3781 catatcgagt ctgcgatcct ggataaaaac gtccagatag gcgaaggcgt ggtgatcctg
     3841 cctttcccgc gcgattctgg cgatattgat catccacttt accatgtgcg cgatggaatc
     3901 gtggttatcg caaaggatac agaagtgccg gccggtacga ggatcgcgcc agaatagttt
     3961 gtaaccgtat cccttttatt ttgtcttaca tataagcagg tgacaatctc tactggttgt
     4021 cacttcgtat ttatcttaat aagaaaggcg tattaaatat aaatcatgga attgtattcg
     4081 gtgattttga tcgtagttca attgattttt ttggagggta tcttatctat tgataatgcc
     4141 gcggtgttgg gcgccatggt tgcgcctctc ccggatgacc aggccatccc ctggccagcc
     4201 tggcttcagg gcatcggtga acgcctggat cacctgcttg gcaatcaacg cctggctgcc
     4261 ttgcgcgtag gtttgctggg agcgtacgtg ggacgcgggg ccatgttgtt catcaccagt
     4321 tttctcatcc acaatgaatg gatccgattg gtcggcgccg cttatttgat ccgcctggct
     4381 tttgatgaat taggggatac cactccaaat gaagtgaccg agcaggagaa aaaggatgca
     4441 attaagggag tttctttttg gtctgtggtt ctaaccgtcg aattaatgga cctcgtcttt
     4501 agcatcgaca acgtcatcgc ggcagtctcc ctttcggatg aaatatgggt tgtgatgctc
     4561 ggtgtaggta ttggtatcct tgcgatgcgt tttgcagcgg gtttattcag ttatgcagtc
     4621 caacgtgagc ctgtcttgaa aatagcagcg tatatattgc tgctcaatat cgggatccaa
     4681 ttgattttgg agcaactatg gaaagtggaa atctcagata tcacccgttt tgctgtttcg
     4741 atggggatca tccttgcctc tttggcatat gcccatagcg catttttaca aagattccaa
     4801 ttcatttttt catggttatc actgggcatg ggattgctga atgaactcat caattggatt
     4861 ttccttccag tcaaagcgtt gtttcaatgg attgcgaatg gattcaagcc ggtaaagctc
     4921 gcagagatcg aatgattgct ccagcttgac atgcgtattc ccgtcgatta aactgacatc
     4981 ggttggggag tagccatggc gcacatcgag cgccaacggg ttatcgtcag gacatggcta
     5041 caaatcagcc atcggtagcc cgaagcgctc tgcctcgtgg gcaagcgttt ggcgagacca
     5101 tcacaaaggc gtgatggtct cgttttaatt tgcctgacca gtggcacgcc tttactttat
     5161 taattaggag atgatgcaat gcctcacgac ggtgaaaaca caaaacagga atggcatgca
     5221 ctgacggctg aagaagttct caaccatctt aaggtggaag ataatggctt aaattcagaa
     5281 gaagcccgga aacgattgga tcattatggt ccaaatcaat taaccgaggc gccccgccct
     5341 acgttcctgc agatgttgtg ggaacaactg aataattttg ttgtcattct attgatcgtg
     5401 gcttccgtca tctcggctgt actcggagat tatgttgagg ctgcggcaat catggctatc
     5461 gtcgtattga atgcgatcct gggaattgtt caggaacggc gcgccgaaga agcacttgct
     5521 gccctcaaaa aacttgctgc accggatgca caggttttac gtgatggacg cagacaatcg
     5581 attcccggtt atgagttggt gccgggtgat attgtgttcc ttgaggcggg taattttgtt
     5641 cccgctgata ttcgtttgct ggaggctatc aatttgcgaa tcgaggaggc ttctctcacc
     5701 ggggagtctc tcgcagttca aaaaaatgct gcaagtgtgc tggataaaaa tctaccctta
     5761 ggcgaccgaa agaacacagc gttcatgggg accgttgtat cttatggaag aggacgcggg
     5821 gtggttacca gcacggggat gcacacgcag ctcggactca ttgcgaccat gctccaaaat
     5881 gtggagaccg aggaaacgcc cctacagcga aggctggatc aacttggcag gtcgttgagc
     5941 atcgttgcat tgttcctggt tatcatcgtc tttatcgttg cactgatcaa ttacacggat
     6001 acgactcagc ttttttcgca gccactcgaa tatcttcgcg agtttggagc ggatatcacg
     6061 gaagtcttta ttattgctat tagccttgcg attgctgcag tgcccgaggg tttgccagcc
     6121 gtcgtgacca tctctttggc cctgggaatg cgcgagatga tcaagcgcca cgcgctcatc
     6181 cggaaactat cttcagtgga aacgcttggg tccgccactg tcatttgttc tgacaaaaca
     6241 ggcacactga cccaaaacga aatgacggtc accgagctgt gggtggatgg gcaatttatt
     6301 aacattacag gcacaggcta tgcccccaag ggcgattttc tggtagatgg gaaacaagtt
     6361 gagataaaaa attaccccgc cgcgctgacc gctttatggc ttggcttgct taataatgat
     6421 gcggagatcg aaatcaccgg cgaaagtgaa cagaacaaga cctatcgtat tgtcggggac
     6481 ccaaccgaag gtgcacttgt ggtggctgct gccaaggcag gcgcgatcca tgtggaaatc
     6541 aaggaagcct acccgcgtga gaatgaagct ccgttcgact cggaacgcaa gcgtatgatc
     6601 acgattcatg atgttcgctc cccacgcgcg catgatcctt ctccttttga tgaacaactt
     6661 aaagactggg atgtcattgc agtcaaagga gcaccagacg ttgtgcttga actttgcact
     6721 caatatcagg gcagagacga cagtcagcag cctttggatg atgtggggcg tgagcgcatc
     6781 ctggccgcga atgacagaat gaccaaagag gcactgcgcg tgctgggggt tgcgtatcgc
     6841 attgccagag acgtgcccga taacccggaa catatcaaga cggaagaact ggagcaagac
     6901 ctggtttttg ttggtctact gggcatgatc gatccgccgc gggcccaggt gaaacctgct
     6961 ttagcgcacg cgcgtgacgc gggtattcgc accatcatga ttaccggcga ttacccaaat
     7021 acagcgcgcg ccattgctga gagtatagga atgcttcaac agggccgaca ggtgatgacc
     7081 ggcgcgcagc tcgatgcgat ctctgaccag gaactccgaa atgtgatcaa agatacggcg
     7141 gtcttcgctc gcgtttcgcc cgaacataaa atgcgcattg tagatgccct tcaagtgaac
     7201 gatgaaatcg tcgccatgac cggagacggc gtaaacgacg cgccggccat taagcgggca
     7261 gatatcggca tcgctatggg catcacggga accgatgtcg ccaaggaaac cgcggacatg
     7321 gtacttacag acgataacta tgcaagcatc gtatcggctg tagagcaggg gcgcatcatt
     7381 tactcgaaca ttcgcaaatt tgtgtttttt ctgctttcgt caaacctggc agagatcatg
     7441 atcatcttcc ttgccacgtt ggcggggctc cctgcgccgc tgaccgcgat ccagttgtta
     7501 tggctcaacc tgattacgga tggtgcaccc gcacttgcgc tcgcggtcga aaaaggcgac
     7561 ccggacatca tggagcaaaa gccgcgtgca aagtcagagc cgatcgttaa tcgcagcatg
     7621 acgattggtc ttgttgtgca gaccatcgca caaaccgggg ctgtgctgac tgcgtttgct
     7681 ctgggtctgc tatggcatct tgaggcaggt gcaacggttg tcgggaatcc gatcgcctac
     7741 ctcatcgctc atgattggcg tggcatcgat gtgcagactg cagagaccat ggcgtttgtc
     7801 acactttcac tggctgaact gttccgtgct tatacagttc gttctgaacg cgcttccatt
     7861 ttccagatcg gtatcttctc caaccgttat atgcaatacg cggtaggtct ctcaatcgtc
     7921 ttgcttttgc tggtcgtgaa cgttcccttt ttgcagccca tcttcaatac acattcccct
     7981 aatctacgcg agtggggtgt cgttctggga ctgtcgatca tcccggcggt cgcggaagag
     8041 atcaccaaat tctttctgcg gcggcagtcg tagatgcggt gcgcgtagtg tgccgcacat
     8101 gtacgacgtg aaaacacaag taggcgagat gacctcgcct ctacgattta agcgaaaaag
     8161 attttcgatg tatagaggaa taaccccatt gtttcatcgc gagacgatgg gcctgcgttc
     8221 cgtacccttt gtgttttccc aacccatagt ttggatactc tgaatccaat tggttcatat
     8281 atgcatcgcg ggcggtcttt gccagcacag atgcagctgc gatgctcagg caacaggcgt
     8341 cgcctttgac gagcgaagtt tgaccaagct ctacttgagg cagctcgagt cgaaaatcgg
     8401 tgagaaggta ttccggcgaa atggataaat tttgcaaagc gcgcaacgct gccaatctgg
     8461 tcgccggaac gatgccctgt gtatcgatct cttcggcaga cgcgaatgct acactccagg
     8521 ctaccgcgat ttcctggatg cgcggcgcca gggcatcgcg ctcgagggga gtcatctgct
     8581 tggagtcgcg gaccccggcc aacgtccgtg caagacgagt cttgtctttt ggcaggatca
     8641 ccgcaccgac tgcaactggt ccggctaagg cgccgcgtcc ggcttcatcc aacccggcaa
     8701 catattgata gcaggaccag agtcgctttt cgtagttgag attagggttt acgatcataa
     8761 agtccacgca ccgcgttcca gcgtatgatc agcaggtcaa caaacattcg ccacgcatcg
     8821 cgcagtactc gaaccttgct atccgcgttg aaattccaat tgatcgggat ttccacaatg
     8881 cgatagccgc ggcgatgagc aatgaaaagc aattccacgt cgaaggccca gccggtgatg
     8941 gtttgaagcc gaaagatatc gtgtgccaca accgcgcgca tacacttgaa accacattgt
     9001 gtatcctgaa gtcctggtag caccatcaac cggatcaacg tattgaatac gcgtcccgtc
     9061 agatgacgat agtaaggctc gttataacga acggagcccg gggcttcgcg cgaaccaatg
     9121 gcaatatcga aatctgtcag ggcagggggg agaaatttcg tgatttcctc aatcggcatg
     9181 gaaaggtctg catcacagat aaagacatat tcgccctgcg actcgcgaac accgcgcagc
     9241 acggcgcttc ccttcccgcg ttgctcactg tgaaacacgc gcaggttttt atgctttttt
     9301 gcaaagttgc ttgcgacttc aagagtttta tctgtactcc cgttttcaac cacgattact
     9361 tctgctgtat acgattgctt ctcaagaaaa cgaaacacct gctccagtga attgggcagg
     9421 cgattctcct cattatatgc agggataatg atcgataaaa aggaactcaa agcggcgtta
     9481 ggttctggtg aaaataatat aaccgaaagc cacaaggatg cataccagcg ccagtgccag
     9541 cccggcgaga atggcaagct gtgcgggtgt cataccaaga attttgctgc gttttcgtac
     9601 gggctcttgc gaagggcttt cagaatcggc ttgagggaga gtggcaggtg gtatatctgt
     9661 gtagggctct tcctccatca tctcaaattc aaaatcagcc gggttgaacg gttttgcatc
     9721 ttctgaggca tcaaagatcg cgcgggcacg cgagacatcc tgcggctggt acggtgcctg
     9781 ttcccattca ttgcgcggcg ccgtgatgac cgcggaggag gccggcgaat ctttctttga
     9841 cggccgcggg ttaatgagac gctctgtgta atcaaatacc tgttcgggcg ccaatatggc
     9901 agtcccgatt gccagactag acacaaatga cttaatacca tcgatcatca tcgccttgag
     9961 cgcgggctgc acggaagaaa tgtgtaggcc ggggtcacca gcaatcaaca ccggctcagg
    10021 agccgcaggg agtctcacac cctggcgttc aataaaaacg cgtacggcgt ttgccatgcg
    10081 gatcgtttgt tgaaccaagt tgacaggggc aggcttatat ttttcgccag cttccacgtt
    10141 ccacatatcg cccctcacct cgtatctgcc acgcacattg gcgatttgaa tgacatggat
    10201 tccggtcgga cccagcaaga tcatcggcac gataatccca ctctgcccca ggggaatgtt
    10261 gcgaatcagg gtgtagcctt tttcgagttg acggtcaaag aatgtgatga ttgctttctg
    10321 agtctgaagt tcgttgggcc agttaaagcc aaattgaaac ataccctgga tgcgttgaga
    10381 gaatccaagt tcgccttttt cgtcaagaag aggagtctta tcaattattt tcatgcatgc
    10441 gtttgctttc gctggcgttc tcgtcagcgg atactaaaaa accggtgctt gttccagttc
    10501 atcacgatcc gcttcattgc gcgtcgcgat aatttccttc ccatgatgat gtagtacttc
    10561 cacgggtttc cccatttcaa aggtgcttgc taaaagttct ttacgttccc caaccacaaa
    10621 ctttttcccg tttttgatgc gttccagcag ctctttgcgg tctagaaaaa catgatctga
    10681 aaaagtagca ccgcggcgct catacgcacg caccgcgacg atttgcccgt tctcataacg
    10741 tgcagcggta atgacaccat cgatcttttt tgccataatt aactccgcat gtattttagc
    10801 acacatgcct tttaagcaca gaaacaggac atgtattgcg aatctctacc gcaaaaatta
    10861 aggcattccg atcaaccagc atatggggga tttccccaat tgcccgccct gcctcagaaa
    10921 catataatgc aaattggtta ctggttcttc atggaacctc ttcggacaaa agtgagtaca
    10981 gccttttgtt agctccgccc caggactggg gcggtttgct ttaacagcac tgccccggct
    11041 gtgagccaat atagaaatcc ctttgtctga tacaaacaga accgccgtat ctaaccgata
    11101 cggcggtttc aagtcagggc gct
//
LOCUS       DASTJP010000107         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1413197, whole genome shotgun sequence.
ACCESSION   DASTJP010000107 DASTJP010000000
VERSION     DASTJP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413197"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /gene="flgC"
                     /locus_tag="VFQ23_03275"
     CDS             complement(<1..399)
                     /gene="flgC"
                     /locus_tag="VFQ23_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HET9905626.1"
                     /translation="MSFWQALRIGSSGLTAQRLRLDLISNNIANAQTTRTEKGGPFQR
                     QDVVFVAEGENPALPRPVAARRGNLGMLQGGVKVAEITTDTEAGPRVYDPTHPDADAD
                     GYVNQPNVDIVVEMTNMLSATRSYEANLASI"
     gene            complement(610..984)
                     /locus_tag="VFQ23_03280"
     CDS             complement(610..984)
                     /locus_tag="VFQ23_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HET9905627.1"
                     /translation="MYQPTYHNQYRKQDVMNASPLRLVIMTYDLAIRACEQQDFAKAA
                     KTISALRDALDLDYPEVSVGLFRLYQWCLDCIRKYDYASAATTLRELRSAWVATEQTL
                     AARQSVVTNAPAYSISLGNILT"
     gene            complement(1185..1502)
                     /locus_tag="VFQ23_03285"
     CDS             complement(1185..1502)
                     /locus_tag="VFQ23_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HET9905628.1"
                     /translation="MSENSINPISHVQPAAIGGMPPKPQVVKTAVEEVSDSDKPPQQQ
                     TEHEPAPASSASNVSVHFRVNDDTHELTVFVVDRENKKILRTIPASEFSKLNAGDILQ
                     LTA"
     gene            complement(1601..>3153)
                     /gene="fliD"
                     /locus_tag="VFQ23_03290"
     CDS             complement(1601..>3153)
                     /gene="fliD"
                     /locus_tag="VFQ23_03290"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HET9905629.1"
                     /translation="DVRRGVYTDVKANFDALQSALQALISTQASYGMNLVSKATVTPG
                     TAGTTVLSATSTESAAAAEYDIAVTQLAKAQSKSTTAAFSPDVALGKSGTFWMGGNGT
                     SAVNLTPDTTVAGAIASTTASGQRELGTGSYTLESRESAGIRQFRLVNADGKAVSIRS
                     QSGTTYTSDWQNMSDGSYDTGRGLNFSLNSLGTTSRTSLTYTAAGTSITINSTDTLRN
                     ITSAINTAGQPEGRDFRASIVANKLVLTGAQSGENHTMLYTDGAGLGFGADMQPAQNA
                     EFTVNGMEVSRATNTNLTDVVDGATISLASDAEGKSARLSISAGSEKAASLMKAMVDK
                     FNAALTHLKDKLASTSATNAEGKTTYTRGPLSGDLGFSGLRLDMINRINRSMTNSGSF
                     KNFSEIGISFDKDMKLTLDSSKLSDALKNHASDVTALLDTGLGDLHSLVSRYAGSKGS
                     LASTLTTIEDQRKSYDKRIAKYNENLTIRKQTLYNQYLGFQTQIADYGRTAEWLGMIT
                     GTNISSSG"
BASE COUNT          752 a          783 c          784 g          834 t
ORIGIN      
        1 gatcgaggcc agatttgctt cataggaacg ggtggcggaa agcatgttgg tcatctccac
       61 cacaatatcc acattcggct ggttgacata accatcggca tccgcatccg gatgggtcgg
      121 gtcgtacact cgcggaccgg cttcggtatc cgtggtgatc tccgccactt tgaccccgcc
      181 ctgcagcata cccaggttgc ctcggcgggc agccacaggc ctgggcagag ccggattctc
      241 accttccgcc acaaagacaa catcctggcg ctggaagggg ccgccttttt cagtgcgggt
      301 ggtttgcgca ttggcaatat tattggagat cagatccagg cgcagccgtt gtgctgttag
      361 cccagaagaa ccgatacgaa gtgcctgcca gaaactcata gatatgctcc ttgttggctg
      421 gataatatct cagcaggcac aaatagacag taaaagcagt gtgaacttaa aataaaattc
      481 gtgtgaagaa acacaaagcg ccatttccct tacggaaagg cgctttgtgt ttcttattca
      541 tatcttcact tcagatcaag ggcaaatgta agccttctag cttcatcacc acttttgtga
      601 taccacaaat caagtcagga tattcccaag agaaatggaa tacgcgggag cgttggtgac
      661 aacactttgt cgagcagcaa gcgtttgttc ggttgcgacc caggcactgc gcaattctct
      721 cagggtagta gcggcggagg cataatcata tttgcgaatg cagtccagac accattgata
      781 gaggcggaat aggccgacgg agacttcagg atagtccaga tccaaagcat ctcgcagggc
      841 actaattgtc ttggcagctt tggcaaaatc ctgttgctcg caagcacgga tggcgagatc
      901 gtaggtcatg atgaccaaac gaagcggact ggcattcatg acatcctgtt ttcgatattg
      961 attgtgataa gttggttggt acacggtttc agctccttga aggattacct gcgtattctt
     1021 gtatgcaatt agattcggct gaattctttc gagcttgaat gctaggccat taattcaaca
     1081 taaatttttt atggagtcga gaagaaaaat agagaacgca acaagcatct cgcctcttaa
     1141 aagagaatcg cattgaaaga tgttattgcg gatcattcac aggttcaggc agtcaactgc
     1201 aatatatcgc cggcattcaa tttggaaaat tcactggccg gtatggttcg caagatcttc
     1261 ttgttttcac gatcgacaac aaacacggtc agttcatgcg tatcgtcatt tacgcggaaa
     1321 tggactgaaa cattggaagc gctgctggcc ggcgcaggct catgttccgt ttgttgctgg
     1381 ggtggtttat ccgaatcgga gacttcttcc accgcggtct tgacaacctg gggcttggga
     1441 ggcataccgc ctatggcagc cggttgtaca tgactgattg ggttgatcga gttttcactc
     1501 ataacacacg ctccttgcat ccttgcatcg gattacaaaa actctatctt tctgcagggc
     1561 aaacctcatg cttgcagctg cactttttta ccttatatta ttagcctgag ctactgatat
     1621 tcgtgcccgt aatcatgcca agccattctg cagttcgtcc gtaatccgcg atctgggtct
     1681 gaaagcccag gtactggtta tacagagtct gttttcggat cgttaaattt tcattgtact
     1741 tggcaatgcg cttgtcatag cttttgcgct gatcttcgat tgtggtcaat gtactggcta
     1801 gtgagccctt ggagcctgca tagcgcgaga ccagactgtg tagatcaccg agtcccgtat
     1861 ccagcaacgc ggtcacatct gaggcatggt tcttgagtgc atcactaagc ttggagctgt
     1921 cgagcgtcag tttcatatct ttatcgaaac tgatgccaat ctctgaaaaa ttcttaaagc
     1981 tgccactgtt ggtcatgcta cgattgatac ggttgatcat atccaatctc aaaccgctga
     2041 agccaagatc gccgcttaga gggccgcgcg tataagtagt tttaccctct gcgtttgtcg
     2101 cagaagtgga ggctaattta tccttcagat gagttaaagc tgcattgaat ttatccacca
     2161 tcgccttcat caaactggcg gctttttcgg atccggccga aatgctcagg cgagcgctct
     2221 tgccttccgc atctgaagcc agactaatcg tggccccatc cacaacatcg gtcaggttgg
     2281 tattcgtcgc ccgcgaaact tccatgccat tcacagtaaa ttcggcattt tgagcaggct
     2341 gcatgtcggc tccgaagcca agccccgcgc catccgtgta aagcatcgtg tgattttcac
     2401 cagattgtgc accggttagc acgagtttat tggctacaat cgaggccctg aagtcacgcc
     2461 cttctggctg gccagctgtg ttaatcgcgg aggtgatgtt gcgcaaagta tctgtcgagt
     2521 tgatcgtgat cgaggtgcct gcagcagtat aggtgagcga tgtcctggaa gtagtcccca
     2581 gtgaattcaa ggagaaattt aggccacgcc cggtatcata gctgccgtcc gacatgttct
     2641 gccaatcact tgtataagtg gtcccgcttt ggctacgaat ggacactgct tttccatcgg
     2701 cgttgaccag gcgaaattgg cggatcccgg ctgattcacg agattccaac gtataacttc
     2761 ctgtgcctaa ttcgcgctgg ccgctagccg tagtactggc aatagcgcct gcaacagtgg
     2821 tatcaggagt aagatttact gcgcttgtgc cattcccacc catccaaaag gtgccactct
     2881 tccctaaagc tacatccggg ctaaatgccg cagttgtgga tttggattga gctttagcca
     2941 actgagtaac tgcgatatcg tattctgcgg cggcagcact ctctgtgctg gtagcactca
     3001 atactgttgt accagcagtt ccaggcgtaa ccgtggcttt gctgaccagg ttcattccat
     3061 agcttgcctg ggtactgatc agagcctgaa gcgcactttg cagggcatca aaattggctt
     3121 ttacatccgt atagacacca cgacgcacat cga
//
LOCUS       DASTJP010000108         7418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_909910, whole genome shotgun sequence.
ACCESSION   DASTJP010000108 DASTJP010000000
VERSION     DASTJP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909910"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2659)
                     /locus_tag="VFQ23_03295"
     CDS             complement(<1..2659)
                     /locus_tag="VFQ23_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET9905630.1"
                     /translation="MSRAFKLVREQNIPELNSVAQLYVHKRTGARLLSVINDEENKVF
                     SINFRTTPKDSTGVAHILEHSVLNGSEKYPVKEPFVELLKGSLATFVNAMTGPDITFY
                     PVASQNLQDFYNLIEVYMDAVLHPLISEQTLMQEGWHYEINDPSEPLTYKGVVFNEMK
                     GVYSSPDSVLSKRIGQSLFPKHIYGVDSGGDPRHIPDLTYENFKKFWETYYHPSNAFI
                     YFYGNDDPEKRLKLMEGYLKPYKKKRVKSVVPLAKSFKRPKQVQDSYASGDDQNIEKK
                     NYLTVNWKLPDTSDPVLNYSLRILGHILIGTPASPLRKALLDSGLGEDLAGLGMSTSQ
                     RHIVFSVGLQGTRARHAKKIERLILDTLKSLAREGIDPNTITASMNTVEFRLRENNTG
                     SYPRGIFILTRILTTWLYDEDPFKLFAFEAPLNQIKSTLTTDPRYFEGLIQTHLLDNA
                     HRTTLRLKPDPELGKRLEEEEKARLAKIRESLSESQIAEIVEQTRKLKLRQETPDSHE
                     ALESLPVLELKDLEKRIKTIPNQELQIENTKVLFHDIFTNGIVYLNLGFDLRAIPLEH
                     IPLIDVFARALLETGTATEDYVQLLQRIGKSTGGIHGGSLSFTTLQTRESRAYLMMSA
                     KAAVGQSAEMMDILKDILLTANLDNRERLKQIVLEEKAGLESSLAPGGTRYINMRLRA
                     QFGGANWINDQTKGIGHLFALRELANDIDKSWKKVLKKFEEIRSTLINSKSLLVNVTL
                     DSENWQTFKPYLEKFIHALPAEDIPLHDFNIPSVIQKEGMAIPAQVNYVGKGANLYDL
                     GYKLDGSSEVVLGYLRMAYLWEKIRVQGGAYGAYVQFDESSGVISFLSYRDPNVASTL
                     ESYDKAAAFLKGLDGSRLSENELKKAIIAA"
     gene            complement(2719..3873)
                     /locus_tag="VFQ23_03300"
     CDS             complement(2719..3873)
                     /locus_tag="VFQ23_03300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET9905631.1"
                     /translation="MNKLPTPTRDMNAVSSSEKSFFKFIDMSLPVNQILHGDCIEVLK
                     SLPENSVDLIFADPPYNLQLHHDLYRPNLTKVDAVKDRWDKFGSFSEYDAFTRQWLSA
                     TQRVLKETGTIWVIGTYHNIYRVGSIMQDLGFWFLNDIVYIKNNPMPNFRGVRFTNAH
                     ETLIWAQKNKGAKYTFNHSSMKKLNDDSRENGLQMRSDWNLNLVNGKQRIKSNGVKAH
                     SAQKPEALLYRIILSSSNPGDVVLDPFFGSGTTGAVAKKLGRTWIGIEREKKYIKIAQ
                     KRIEAVKEADSEAVEIERRKQVRVPFGALLENGIVRPGQVLYFAKNSTKAKILSNGHL
                     RCGEHTGSIHGVARSLLNGAPANGWDLWLFKDDSGKKIPIDTLREKFRSK"
     gene            complement(3797..4828)
                     /locus_tag="VFQ23_03305"
     CDS             complement(3797..4828)
                     /locus_tag="VFQ23_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9905632.1"
                     /translation="MKAALFQRCGDASQIQYADVPEPQIRAGQVLIRVHACAFNHLEL
                     WALHGPADESYRFPMWTGSDIAGVIESSASDVTGWSVGEAIAVNPSLSCGACDRCLEG
                     EISQCDEYDILGSNGNGGNAEYIAVNADKLIRMPDGFDFVKAAAAPLAYQTAWRALVR
                     RAKLQQGEDVLILGASGGVAAAGIQIAKMLGARVFAVTSSEEKMKKAQGAGADVVLNR
                     KTSNPFDEVRRITNGRGVDLVVENVGAATWSESQKILRKGGRLVTYGRTTGREAITNL
                     STLFWNEQTLIGSTMGSLNDFREVMEQVFNGAIYPIIDSVYPLEQAADAYARYERGEQ
                     FGKIVLQIH"
     gene            complement(4956..5231)
                     /locus_tag="VFQ23_03310"
     CDS             complement(4956..5231)
                     /locus_tag="VFQ23_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905633.1"
                     /translation="MSQDQQQYCPICRTSVYFSPRYPRHLCVDCASRAKSKDGRLLKF
                     MNESMSGGYIAQYADTGESYPSHECYVDGVECYADEDYWGGIVIQTV"
     gene            5414..6517
                     /locus_tag="VFQ23_03315"
     CDS             5414..6517
                     /locus_tag="VFQ23_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9905634.1"
                     /translation="MTSLNDKSFPYSRKLGRIGLLLCILLMLPSLLGCGAVNMINPPT
                     ATPTNSRTPTASPTSTLTPTPTLAPTPTLQPAACGGPRVMFILLIGSDARAKSYNAGL
                     ADSIRIVRVDFVEPRVQLLSFPRDLYVEIPGIEEHGGITHGKLNQSYLYGNPGLNYFD
                     GKGQGPGLLAATLEHNFGTQIDHYIAVNIQSFARIVDALGGIDINLPFVVDGRVKGSR
                     NSDVYFEAGRQHLNGYRTMLLARMRPLGDFQRNAVQNMILQSLAEKMLKRSALRKLPE
                     LIDAVKDSVQTDIGVVEAGQLLCLATELDTRDIEYINFPETIFSLGRVNDPVLGNTSI
                     VEANFDVLRIYTQKFNHGTELEPDDGMMNEAYR"
     gene            6631..>7418
                     /locus_tag="VFQ23_03320"
     CDS             6631..>7418
                     /locus_tag="VFQ23_03320"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HET9905635.1"
                     /translation="MTATLLLEDGTVIEGGAFGAEADAIFELVFNTSMTGYQEILTDP
                     SYRGQGVLFTVSHVGNVGINLEDDESVKPQVSAAVIRSLSPVVSNWRSALSLSDWLMG
                     HGVPGISGVDTRWLTRKLRDRGTQKAALSTKGTPADELLRMTREWPGLDGRDMVKEVT
                     CHETYHWVDDEGKKWVKQNANGKRPNVIAYDFGIKENILRHLSSNGAIVTVVPADTTS
                     GEVLALKPDGVFLSNGPGDPAGLPYAVESVSGLIESGVPMFGICL"
BASE COUNT         1734 a         1893 c         1823 g         1968 t
ORIGIN      
        1 tagcagcgat gatggctttc tttaattcat tctcagagag acgtgaacca tccaaccctt
       61 tcaggaacgc tgccgcttta tcatagcttt cgagggtaga agcaacgttc gggtcgcggt
      121 acgaaagaaa actgatcaca ccactgcttt catcaaattg aacgtacgcg ccgtaagctc
      181 cgccctgcac ccgaatcttt tcccaaagat acgccatacg caaatatcca agcacaacct
      241 ccgatgagcc atcgagctta taacccaggt catagagatt tgcgcccttg cccacataat
      301 tgacctgggc gggtattgcc atgccttctt tttgaatcac tgatggaata ttgaagtcgt
      361 gtaacgggat gtcttccgcc ggtaaagcat ggatgaattt ctcaagatac ggtttgaacg
      421 tctgccagtt ttccgaatca agcgtcacgt tgacaagcag gctcttactg ttgatcaggg
      481 tactgcgtat ctcctcaaat ttcttgagca cttttttcca tgatttatcg atatcattgg
      541 caagttcacg caaagcaaac aaatgaccga tgcccttcgt ctgatcgttg atccagttcg
      601 ctccgccaaa ctgcgcgcgc agacgcatgt tgatataacg cgtcccgccg ggagcaagtg
      661 acgattcaag tcccgctttc tcctccagaa cgatctgttt caatcgctct cggttgtcaa
      721 ggttggcggt gagtagaata tctttcagga tgtccatcat ctctgcagat tgacccaccg
      781 ccgctttggc gctcatcatt aggtacgccc ggctttctct ggtttggaga gttgtgaaac
      841 taagcgagcc gccgtgaatt cctccggtac tttttccaat cctttgcagg agctgaacgt
      901 agtcttcggt ggcggttccc gtttcaagca acgcacgtgc aaacacgtcg atcaaaggga
      961 tatgttccag tggtatagca cgcaggtcaa accccagatt gagataaaca ataccattcg
     1021 taaagatatc gtgaaacagc accttcgtat tctcgatttg cagttcctgg ttcggtatcg
     1081 ttttgatcct tttctcgaga tcttttagct caagcaccgg caggctttcc agggcttcat
     1141 gtgaatcagg ggtctcctgg cgaagcttta gcttcctggt ctgctcgact atttcagcga
     1201 tctggctctc actcagtgac tcgcgaattt ttgccaatcg ggctttctct tcttcctcga
     1261 gacgctttcc caattcaggg tccggtttga ggcgcagggt tgtccgatgc gcgttgtcca
     1321 gtaagtgagt ttgaatgagc ccttcaaaat agcgcgggtc ggtggtgagt gtggatttga
     1381 tctggttcag aggtgcttcg aaagcaaaca gcttgaacgg gtcttcatca tacagccagg
     1441 tggtcaggat gcgggtcaga atgaaaatgc cgcgcggata ggaacctgtg ttgttttcgc
     1501 gcagtcgaaa ctccaccgta ttcatcgaag ccgtgatggt gttgggatca atcccctccc
     1561 ttgcaagact cttcagcgta tcgagaatca acctctcgat ctttttcgcg tgacgggcgc
     1621 gcgtgccttg cagacccacg gagaagacaa tatgacgctg gcttgtagac attcctaagc
     1681 cagccaggtc ttcaccgaga cctgaatcaa ggagcgcttt ccgaagtggg gaagcgggtg
     1741 taccgatcag aatgtgaccg agaatgcgca ggctgtaatt caagaccggg tccgatgtat
     1801 cgggcagctt ccagttgacc gtcagatagt tcttcttttc gatgttctga tcatcacctg
     1861 aagcatacga atcctgcact tgttttggcc gcttgaatga ctttgccagt ggcacgacag
     1921 acttcacgcg tttcttctta tatggcttta gatagccttc catcaacttg agcctttttt
     1981 cgggatcgtc attcccataa aagtaaatga aggcattgga gggatgatag tacgtttccc
     2041 aaaacttctt gaagttttca tacgtcaggt ctgggatatg gcgagggtca ccgcccgagt
     2101 cgacgccgta gatgtgtttt gggaagagcg attgtccgat gcgtttactc aggacactgt
     2161 ctggcgacga atagacgcct ttcatctcat tgaacaccac tcctttatag gtaagtggtt
     2221 cactggggtc gttgatctca tagtgccagc cttcctgcat cagagtctgt tcgctgatca
     2281 gtggatgcag caccgcatcc atgtacacct cgatcaggtt atagaaatcc tgcaagttct
     2341 gactggcaac cgggtaaaag gtaatatccg gccctgtcat cgcattgaca aaggttgcca
     2401 gcgagccttt gagcaactcc acaaacggtt ccttgaccgg atatttctct gagccgttca
     2461 atacgctgtg ttcaaggatg tgggccacac ctgttgaatc ttttggcgta gtgcggaagt
     2521 tgatgctgaa tactttattt tcctcatcgt tgatcactga aagcaatcgc gcaccagtgc
     2581 gtttgtgcac gtagagctgc gcaacggaat tcaattccgg gatgttctgt tctctgacta
     2641 gcttgaaagc tcttgacatt gttgttccat ttagcctttc acaataatga tgattgtaaa
     2701 gtggtatgcc atcccaccct acttacttcg gaatttttct ctgagcgtgt cgattgggat
     2761 tttcttccca ctgtcatctt tgaatagcca cagatcccag ccattcgccg gcgcgccgtt
     2821 caacagagat cgcgccactc catgaatcga ccctgtatgc tcaccgcagc gaagatgtcc
     2881 gttcgacagg atttttgctt tggtactatt ctttgcaaag taaagaacct gcccgggcct
     2941 gacgatgcca ttttcgagca gcgctccaaa tggtaccctc acttgtttcc gtctttcaat
     3001 ttcgactgcc tcggaatcgg cttccttcac cgcctcgatg cgtttctgcg cgatcttaat
     3061 atattttttc tctctttcga tgcctatcca ggttcgcccg agtttcttcg cgaccgcgcc
     3121 ggtggtgccg ctcccaaaaa acggatcgag taccacgtcg ccagggttcg acgaggacag
     3181 gataattcga tacaacagag cttctggctt ttgcgctgaa tgtgctttga ctccgttgga
     3241 cttaattctt tgtttgccgt ttactaaatt gagattccaa tctgagcgca tctgcaagcc
     3301 attctcgcgg ctgtcatcgt tcaatttttt catcgacgag tgattgaatg tatactttgc
     3361 gcctttattt ttctgcgccc agatcaacgt ctcatgcgca ttcgtaaagc ggacgccgcg
     3421 aaagtttggc atcgggttgt ttttgatgta aacaatgtcg tttaggaacc agaagccgag
     3481 atcctgcatg atcgagccga ctcgataaat attgtgatac gtgccgatga cccagatggt
     3541 gcccgtctct ttcaggacgc gctgggttgc ggatagccac tgtctcgtga aagcatcgta
     3601 ttctgaaaag ctgccaaatt tatcccaacg gtctttgacg gcatccactt tcgtcaggtt
     3661 gggacgatac aagtcatgat gcaattgcag gttataggga ggatcagcaa agatcaaatc
     3721 caccgagttc tcaggcaggg acttgagaac ctcaatgcaa tcaccgtgga ggatttggtt
     3781 gacaggcaaa gacatatcaa tgaatttgaa gaacgatttt tccgaactgc tcaccgcgtt
     3841 catatctcgc gtaggcgtcg gcagcttgtt caagtggata gactgaatcg ataatcgggt
     3901 aaatcgcgcc gttgaagacc tgttccatca cttctcgaaa gtcgttgagg cttcccatgg
     3961 tggagccaat cagggtttgt tcgttccaaa agagggtgga caggttggtg atggcttcac
     4021 gtccggtcgt gcgaccatag gttaccagcc ttccaccttt acgaaggatc ttctggcttt
     4081 cactccaggt ggcggcccca acattttcga ccacgaggtc caccccgcgt ccattggtga
     4141 tgcgccgcac ttcgtcaaat gggttggatg ttttacggtt cagcactacg tctgcgccgg
     4201 caccctgcgc tttcttcatt ttctcttctg aggatgtgac cgcaaagaca cgcgctccca
     4261 gcattttggc aatttgaatc cccgccgctg ccacacctcc gcttgcccca aggatcagaa
     4321 catcttctcc ttgttgaagc ttggctcgtc ggacgagggc acgccaagcc gtttgatagg
     4381 ctaagggagc cgcagcggct ttgacgaaat cgaagccatc cggcatgcgt atcaatttat
     4441 cagcattgac cgcaatgtat tcggcattgc cgccgtttcc atttgagccg aggatgtcat
     4501 actcatcaca ttgactgatc tcgccttcaa gacagcgatc acacgcgcca caggatagcg
     4561 acgggttgac cgctatcgct tcccccactg accagccggt tacgtcggat gcgctgcttt
     4621 cgatcacacc ggcaatgtct gaacccgtcc acatcgggaa acgataactc tcatccgcgg
     4681 gaccatgcaa tgcccataat tccaaatggt tgaacgcaca tgcgtgcaca cgaatcagca
     4741 cctgcccagc cctgatctgc ggctcaggta catcagcata ttgaatctgg gatgcgtcgc
     4801 cgcatcgttg gaaaagggcg gctttcatgt cttgatgcgc tcaagacaat cgcaatgatt
     4861 acgggaattg aggggtcgtt gattcaccgt tcaaattata cactccccga atcagaatag
     4921 acctgactga tgcaaaacca gcgtccggtg aatcttcaaa ccgtctgaat tacaatccca
     4981 ccccaataat cttcatctgc ataacactca acgccgtcca cataacattc gtggctggga
     5041 taagattctc ctgtatctgc atactgtgca atataaccac cactcatgct ttcgttcatg
     5101 aacttcaaca agcgtccatc tttagacttt gcccggcttg cacaatccac acacagatgc
     5161 cgcggatagc gtggggagaa atatacagag gttcggcaaa tggggcagta ctgttgttga
     5221 tcctgcgaca tattaattat tatcccatga aaaccttccc gcctcctgaa attcgagaag
     5281 aaggaattag agatcgttca ctctaaggcc gctcgtggtc acatcgtaaa atgcggaact
     5341 attccagtct cacgaccatt gtattaatga ggacgcgctg gtattcttgc tcaaattgcc
     5401 ccgaatggag cctatgacct ctctcaatga taagtctttt ccttattcca gaaaattagg
     5461 gcggattggt ctcttgcttt gcattctact gatgttgcca agtttgctcg ggtgtggtgc
     5521 cgtgaacatg ataaatccgc ccacagctac gccgacgaat tcacgcactc ccacagcgtc
     5581 cccaacctct accctcactc caacaccgac tcttgccccg actcccacgc tgcagccagc
     5641 ggcctgcggc ggaccacgcg tgatgttcat cctgttaatc ggcagcgatg cccgggcgaa
     5701 gagttacaac gctggactgg cagactcgat acgcatcgtt cgcgtggatt ttgtggagcc
     5761 acgcgttcag ctcctgtcat ttcctcgaga cttatatgtt gagattccgg gcatagaaga
     5821 gcacggcggc atcacacacg gaaagcttaa tcagtcttat ctctatggca accctggctt
     5881 gaattatttt gacggcaagg gacaggggcc aggtctactg gcagcgaccc tggagcacaa
     5941 cttcggcacg cagatagatc attacattgc ggtcaacatc caatcctttg cgcgcatcgt
     6001 cgacgcacta ggtggcattg atatcaacct gccgtttgtc gtagatggtc gtgtgaaagg
     6061 ttcaaggaat tctgacgtct attttgaggc cggtcgacag catttgaacg gttatcgcac
     6121 catgctgttg gcgcgtatgc ggccgctggg ggattttcaa cgaaatgcag tgcaaaacat
     6181 gatcctgcaa tccctggcag agaaaatgct aaagcgatcc gccctgcgaa aattacctga
     6241 attgatcgat gcagtgaagg attccgtcca aacggacatt ggtgtagtgg aagccgggca
     6301 gctattatgc ctggcgacag agctggacac acgagatatt gaatatatca actttcccga
     6361 aaccatcttc agcctcgggc gcgtaaatga tcccgtgctc ggaaacacgt ccatcgtgga
     6421 agcgaacttc gatgtgttga gaatctatac ccagaaattt aatcatggca ctgagctgga
     6481 gcctgatgat ggaatgatga atgaggcgta tcgctaagaa cagcccaatc tatctggggg
     6541 agctattttc catcgaagca aaagtgtttc ttttcttgat ctttgacgtc tagtccttat
     6601 tggtctagac ctttttggag aagatgcatt atgactgcaa cgctgctatt ggaagatgga
     6661 acggtgattg aaggaggagc attcggtgct gaggctgatg ccatatttga attagttttc
     6721 aacaccagca tgacgggcta tcaggaaatt ctcaccgacc cgtcctatcg cgggcaaggg
     6781 gtgttattca ccgtttcgca tgttggaaac gtaggcatca atttagaaga tgatgagtcg
     6841 gttaagccgc aagtttcagc agcagtaatt cgttctctca gccccgtagt gtcgaactgg
     6901 cgttcggcac tttctttatc tgactggtta atgggacatg gcgttcctgg tatcagcggt
     6961 gtggacacgc gctggctgac gcgcaagctc cgtgaccgtg gtacacaaaa agccgcgcta
     7021 tccaccaagg gcacaccggc tgatgaattg ttgagaatga cccgcgagtg gccgggtctc
     7081 gatgggcgcg atatggttaa ggaagtaact tgtcatgaaa cctatcattg ggtggatgac
     7141 gaaggcaaaa agtgggtgaa gcaaaacgca aatggcaaac gaccaaatgt aattgcctac
     7201 gattttggca tcaaagaaaa tattctgcgt cacctttcgt ctaatggcgc aattgtaacg
     7261 gttgtccccg ccgacacaac ttcgggtgaa gtattggcgc tgaagcctga tggtgtcttt
     7321 ttatccaatg gacctggtga tccggcaggc ttgccgtatg ctgtcgagtc cgtaagcgga
     7381 ctcattgaaa gcggcgtgcc gatgtttggg atctgcct
//
LOCUS       DASTJP010000109         4202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_559523, whole genome shotgun sequence.
ACCESSION   DASTJP010000109 DASTJP010000000
VERSION     DASTJP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559523"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFQ23_03325"
     CDS             <1..393
                     /locus_tag="VFQ23_03325"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HET9905636.1"
                     /translation="GWGGLKASGVPKDIFMLESVPHDWLFPKVSAVAHHGGAGTTAAG
                     LRAGKPTIVCPFVGDQPFWGGIVEQLGVGPKPIRQSRLTTESLAEAIGIATQNATMQR
                     RAEELGSKIRSEDGVTRAVEIVNATAHL"
     gene            866..1870
                     /locus_tag="VFQ23_03330"
     CDS             866..1870
                     /locus_tag="VFQ23_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9905637.1"
                     /translation="MRPTIRDVAKKLNLSITTVSRALDGYDDVADETRQLVIDTARKL
                     GYSPNRAARQLRRQKAETVGYILPAGAQHFAEPFFTEFIAGLGNELAPRNYDLLVSAV
                     ASETEEQEMYRRWAESGKVDGFILNRLRLQDWRVKFLLKQHVPFATLDYSGTGRYPCI
                     RVDGSDGFVELVQHIRQNGFERFAFIGGPANLVSQSERLNWFRGAIQFHELALDDGLV
                     TTADLTSTGGYQSAIELLSLPDPPDAVFCVNDETAFGVLHAAHQRGLAIGQQLAVAGF
                     DGVQEAKHTEPPLTTLDIPVFDIARQLVDMLLKTIAGESVTSPVLIKPNLLIRPSTGG
                     "
     gene            1993..2886
                     /locus_tag="VFQ23_03335"
     CDS             1993..2886
                     /locus_tag="VFQ23_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9905638.1"
                     /translation="MEYDLKEKVIIITGANSGIGKAAAIQLARLGASVVVASRSKERG
                     ERAVDDIRRAANSFEVEFIQVDMSSQESIRHFACVFNQCYERLDVLIHNAANFDHRQK
                     EPVLTQEGLETVFATNHLGPFLLTHLLLDMLKASAPSRIITVSSKGLISYPLLDIEFS
                     NLYGERKFSVQHAYYHSKQAQVMFTFSLAERLQRTGVSVHCVRVGNVAIPDERLTYLP
                     KWILKIYELKRKFSMTPEKMAETYVWLAADPTGEQTTGKYWDAPGMEVRANSNAYNRE
                     TQKRLWAVSELFTSTVEPDLD"
     gene            2949..3842
                     /locus_tag="VFQ23_03340"
     CDS             2949..3842
                     /locus_tag="VFQ23_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9905639.1"
                     /translation="MHYDLKEKVIIITGANSEVGKAACIQLARCNATVIMACRSSEIG
                     AQALVDVRNESNSPRVDLMQVDLSSQESIRQFAREFLERYGELHVLIHNAANLDDSQK
                     KLVLTADGIETVFATNHLGPFLLTHLLLDALKDCAPSRIVTVASKGLISYPLLDIEFD
                     NLNGEQQFTPQHAYYHSKQAQVMFTFDLAERLRGKGVTVHCVRAGDTAIPGEQLARPL
                     EEMLSTNEMKRGSSISLKQIAETYLWLSADLIGEQQTGGYWVAPGVAVTANKNAYNKE
                     TQKALWDISAELTGVHQHAVG"
     gene            3829..>4202
                     /locus_tag="VFQ23_03345"
     CDS             3829..>4202
                     /locus_tag="VFQ23_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905640.1"
                     /translation="MLWVEFIVCAVLIFWVGSLLSKYGDVIAEKTGLGRAWIGAILIA
                     GVTSLPELASGVSAVAWLNAPNLAAGAVLGSCLFNLALIAMIDLAYQPGRVLAKAQEV
                     HILSGGLGVLMLGIVAMGVLIGP"
BASE COUNT         1042 a          992 c         1095 g         1073 t
ORIGIN      
        1 ggatggggtg gtctgaaagc atcaggtgta ccaaaagata tattcatgtt agaaagcgtg
       61 ccgcatgatt ggctctttcc aaaggtttcg gcggtggctc accacggtgg tgccggtaca
      121 acggctgccg gattgcgcgc cggaaaacca acaattgtct gtcctttcgt gggtgaccaa
      181 ccattttggg gtgggatcgt ggagcaactt ggtgttgggc cgaaacccat tcgtcagtca
      241 cgtcttacta cggagtcttt ggcggaagct attggtatcg ccactcagaa tgccacaatg
      301 cagcgacgag ctgaggaatt gggatccaag attcgctctg aagatggtgt cacccgtgcg
      361 gtggaaattg taaacgcaac tgcacatctc taatcaatca caagagcaag cagaaggctg
      421 gatgcgcggg ataaaggcca tctagttgta ttgtgtctaa actcataaga gaagagaaag
      481 agactggagt tgaatatcca taataaaatc aggactcacc ttcgaagcgc atacatctac
      541 cccgctctgg tcactcaaag atgttctggt taggcagtac gccttacctt tctgtctctt
      601 gtaaagatca cccttccttc tggggtttgg gcaactgctc aagcacttgc gagcaatgtc
      661 caacgtcata gcgccttcgc ctgtcagcca caggttcggg caatggattt tcctcatgtc
      721 cactttccca aagcgggcgg ctatatccct ggggttaaag ctgacctaaa agctaatcat
      781 caaacaacga tctcatctct cacgaaaccc tttccatcta aggcgttaga tgttatcata
      841 attatccaaa acgttttgga gaaatatgcg ccctactatt cgagatgttg cgaagaagct
      901 taatttatca attactactg tctcgcgcgc attggacggc tatgatgatg ttgcggacga
      961 gacacgccag ttagtgattg atacagcgcg caaattagga tacagcccca atcgcgctgc
     1021 ccggcaacta cgacgtcaaa aggccgaaac agttggctat atcctgccgg cgggcgcgca
     1081 acattttgct gagccttttt ttacagaatt cattgctggt ttgggcaatg agcttgctcc
     1141 acgcaattat gacctgctgg tttctgcagt agccagtgag accgaagaac aagagatgta
     1201 taggcgctgg gcggagagtg gcaaagtaga cggtttcatt ctaaaccgcc tacgtctgca
     1261 agactggcgc gtgaaatttt tattgaagca gcatgtcccc tttgcaacct tggactattc
     1321 aggcacagga cgttatccat gtattcgcgt ggatggatca gatggcttcg tagaattagt
     1381 gcaacatatc cgtcaaaatg gctttgagag atttgcgttt attggtggac ctgccaacct
     1441 ggtcagccag tcggagaggc taaattggtt tcggggggcg attcaatttc atgagcttgc
     1501 gctggatgat ggactagtta ctacagccga tttgacgagt acaggagggt accagtcagc
     1561 aattgaactt ctcagtctgc ctgacccacc ggatgcagta ttctgtgtca atgatgagac
     1621 cgcctttggt gtactgcacg cagctcacca acgaggactt gcgatcggtc agcagcttgc
     1681 agtcgcagga ttcgacggtg tccaggaagc aaagcatacg gagccgccac tgaccacact
     1741 cgacatcccg gttttcgaca ttgcacggca attggtggat atgctcctga agaccattgc
     1801 cggcgagtcg gtcacatcgc ctgtgctgat caagcccaac ctcctgattc gtccctcgac
     1861 cggcggctag ctgtctcact agggcagtct ctttttttgt cattttccaa aacgttttgg
     1921 aagctcaaag ttattggaat actgtgatgc ggtcgctggc tagcccccca cagtacattt
     1981 gcggagaata ggatggaata cgacttgaag gagaaggtca ttatcataac cggtgccaat
     2041 tcggggattg ggaaggccgc ggcgattcaa ctggcaagac ttggtgcatc tgttgttgta
     2101 gcgagtcgtt caaaagaacg aggtgagcga gccgtggacg acatcaggcg ggcagccaat
     2161 agttttgagg ttgagtttat ccaggtggat atgtcttcgc aagaatccat acgacatttt
     2221 gcatgtgtat tcaatcagtg ctatgaacgt cttgatgtcc tcatccataa tgctgccaat
     2281 tttgatcata gacaaaagga acctgtgcta acccaagagg gtttggaaac cgtttttgcc
     2341 accaatcacc tggggccgtt tttgttaacc catttgctgc ttgacatgct caaagccagc
     2401 gcgcccagcc ggatcattac tgtgtcaagc aagggactga ttagttatcc acttcttgac
     2461 attgagttca gtaatctcta tggagagcgc aagttcagtg tacaacacgc ttattaccat
     2521 tcgaagcagg cccaggtgat gttcaccttt tccctggcag aacgactgca acgaacagga
     2581 gttagtgtgc actgcgtgcg agtgggtaat gttgccattc cagatgaaag gcttacttac
     2641 ctacctaaat ggatactcaa gatctatgaa ttaaagcgca aattctccat gacacctgaa
     2701 aaaatggcag aaacctatgt ttggctcgcc gctgatccga ctggagaaca gaccaccggc
     2761 aagtactggg acgcgccggg catggaggtc cgagcgaatt cgaatgccta taacagagaa
     2821 acacagaagc gtctctgggc tgtcagcgaa ctcttcacaa gcactgtgga acctgatctg
     2881 gattgaactg attctttgtg cagttttcat attctggaca ccccctacgc gcaaggcttg
     2941 gaggacctat gcactacgac ttgaaagaga aagttatcat tatcacaggc gcgaactcag
     3001 aggttggtaa ggctgcctgc attcagctcg caagatgtaa tgcaacggtg attatggcat
     3061 gccgctcgag cgagatcggc gcgcaagcac ttgtcgacgt tcgcaacgag tctaatagcc
     3121 caagggtgga cttgatgcaa gtggatctgt cgtcacaaga gtccatccgt caatttgcgc
     3181 gcgagtttct ggagcgttac ggggagcttc acgtgctcat tcataatgca gccaatttgg
     3241 atgatagcca gaagaaacta gttttgacgg cggatggtat tgaaactgta tttgcgacca
     3301 atcaccttgg gccattcctg ctgactcacc tgctgttgga tgctttgaag gactgtgctc
     3361 ccagccgtat tgttactgtg gcaagcaagg gtcttatcag ctatccgttg ctggacatcg
     3421 aatttgataa tctcaatggc gagcaacagt tcactccgca acacgcttac taccattcca
     3481 agcaagcaca agtcatgttc acctttgacc tggcggaacg tctgcgcggt aaaggtgtaa
     3541 ctgtacattg tgtacgtgca ggtgatacag cgattccagg tgaacaactc gcacggcctc
     3601 tagaagagat gttgtccacg aatgagatga agcgtgggtc ttccatatct ctgaaacaaa
     3661 tagcggaaac ctacctttgg ctgtctgctg atctcattgg agaacagcaa accggcgggt
     3721 attgggttgc cccaggcgtt gcggtcacag cgaacaaaaa tgcctataac aaagagaccc
     3781 agaaagcttt atgggacatt agtgctgaac ttacgggagt ccaccaacat gctgtgggtt
     3841 gagttcattg tttgcgcggt attgattttc tgggtaggct ccctactttc caaatatgga
     3901 gatgtgatcg ccgagaaaac cggcttggga cgtgcctgga ttggagccat tctcattgca
     3961 ggtgttacat ctttgcccga attggcctcc ggagtttccg ccgttgcatg gttgaacgcg
     4021 cccaatcttg cagctggagc agtattagga agctgcctct tcaatcttgc actcatcgcg
     4081 atgattgatc ttgcctacca accggggaga gtgctggcca aagcccagga ggttcatatt
     4141 ctttctggtg gattgggtgt gttgatgctt ggtattgttg caatgggcgt tctgattgga
     4201 cc
//
LOCUS       DASTJP010000110        22672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_40819, whole genome shotgun sequence.
ACCESSION   DASTJP010000110 DASTJP010000000
VERSION     DASTJP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 22672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22672
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40819"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFQ23_03350"
     CDS             complement(<1..450)
                     /locus_tag="VFQ23_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905641.1"
                     /translation="MNKLAHSPHKLSAYIIVVAIYAAYVAVFVLYHEQVGDVIASSAI
                     VPIISASWYFGIAGGILTALFSIAVNIIVQALAGISYSELLTTPSTIIGSGALIITAI
                     VVGRLASLTRERSEALAKLEALEENNQTHTKFLELLNEITGTALEAEN"
     gene            complement(592..1593)
                     /locus_tag="VFQ23_03355"
     CDS             complement(592..1593)
                     /locus_tag="VFQ23_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905642.1"
                     /translation="MDFLKSFFSSPKKPSPPPKIQPAPVNDSTDEPPQVTVLKTLVIV
                     YDPVVEASTGTKLSQFMHWNSVEELAKGYMSDILQVSSGLARYQIVQRIDVDAFPAKV
                     NGYQYDADTYLKVMRGETRPYMPQEADYHAIIERFNVLESVATGEIDEVWVFNFPHAG
                     FYESIMGGPGAFWCNASPLRNTESALRRFVIMGFSYERGVGEMLENMGHRAESIMEKT
                     FENLEEDVNLWKRFTRYEKTHPGRAAVGNVHFAPNSDRDYDWNNPSTVVSECDDWLYN
                     FPNFKGTTREVTAADWGDGDIREHHKWWLSHFPKTAGRRNGIHHNWWQYIMNPNHVG"
     gene            complement(1632..4118)
                     /locus_tag="VFQ23_03360"
     CDS             complement(1632..4118)
                     /locus_tag="VFQ23_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905643.1"
                     /translation="MATFDLNILPIYRINGQESETLPGLLAVTPPRRTARGREKDSLI
                     LYIMLTGNATFLPPELMQLNSQAAGIFYQSSGSLTSAMRKAAEHINTVLLERNLSTTG
                     RGQYALAYLALASVRENQCTLLLSGQTHAVWVSEGKSRHIYEPALSGKGLGSSQSIQT
                     YLSQVELHPHDLIVLCGKFPRDWEADLLNERPPASLDAGYPKLTFTKGDLHAALIQAQ
                     SGLGTISVIRPDLNALHRHVERPEPVKDQPLPQESELQRPEEPKVQNKHEEFIFPRSP
                     YLRGEEPAPVDEPPITEEELDAIADFAAHMVQPSAYAIPPQPEATIPLPTEEPTTASA
                     VPVSRAFPSSIPRTESQPSKPNVEAASQDLDESDSIQLKSDKPSAHAEAARQMAKAMV
                     GGIRSGRRVNERIAAYINKFVPRLLPTMSSKKPLVIPTYIMIFIAVVIPVLVVTAASA
                     VYLRFGQSIQYEELYGQALNARAQAESAAEPVRQRDEWLRVLSLLDQANAYRETDESR
                     LLRTEAQAKLDNLMGVVRLEFSPAFTGGLGNTTQISRMAASESDLYMLDAERGNILHA
                     SFTGRSLNLDTTFNCQPGTHAGYQVGTMVDLLALPKVNAIGATVLGIDTNGNLLYCSP
                     GQVPQAIPLPNLPNTNWGRITAFVLDNGSLYVLDATSRSVWVFVGKDSTFIDTPYFYF
                     SNQIPNIDNAIDLAVSGDDLYLLHADGHLSTCTFSRIDATPTRCVDPATYNDSFPAHL
                     GLDLFTQAHFTQIALTNPPNSVVLLLDSEHQSVYRFTPRSLELQNQVGAHAGKANPFQ
                     SGLVGAMAVSPNYVLYLAIGNQVYFATNLP"
     gene            4301..5314
                     /locus_tag="VFQ23_03365"
     CDS             4301..5314
                     /locus_tag="VFQ23_03365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905644.1"
                     /translation="MNFKLHKDFSEIDASTWNELVQQSIADTPFSRHEYLSEWWQTLG
                     GGEWRQAELVLVSARDNDQLIGIAPLFVAEYEGRQALLLVGSIEIADYLDLIVREPDL
                     SRFLSALFDFLDTSSPSQWSAIDWYNIPDSSPTLAALKAESEKRGWAHHEEIYRPTPR
                     IPLNGSFEEYLSRIDKKQRHEIRRKMRRAAESDLNVRFVIIDKNADIESEIETFFHLM
                     IQDTNKANFLTPPMREQMTVTIRTAFEQDYLWLAFLEVDGTKVAASLNFDYKNKLWGY
                     NSGVSREHMELSPGWVLLAHTIQWCCENGRYEFDFMRGDEEYKYRFGGVNRYVMRERA
                     IKS"
     gene            complement(5315..6805)
                     /locus_tag="VFQ23_03370"
     CDS             complement(5315..6805)
                     /locus_tag="VFQ23_03370"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HET9905645.1"
                     /translation="MTSNEFRIEKDSLGEMPVPAAALYGAQTQRAVENFPITGLRPWR
                     AFVWSIAAVKRAAALVNYELGLFNDREVDGKQFTARQMAESVAQAAEEVMEGKWDDQF
                     VVEPFQAGAGTSHNMNANEVIANRATQILGGELGKYYIHPNDHVNMAQSTNDTIPTSI
                     RLGALWRLDELLESLKNLQVALEAKAREFEIVVKSGRTHLQDAVPVTLGQEFSAYAKA
                     VERNIERVRRSAEGLRRLGIGGTAVGSGLNAHPEYHARMVKKLSEITGLELYESDNLF
                     ESMQSMGDAADFSGSLRTLALDLVRIANDFRLLASGPSTGFDEIRLPAVQPGSSIMPG
                     KVNPVMAEMLDQAMFHVIGCDTTVALAAQAGQLELNVMMPILGHNLFEMMQVVIGSVN
                     AFTERAVKGLTANREKAEGWLAKNAIVVTALNPVIGYSQGAALVKEALANNASIRELA
                     IEKANSGMLKHRDEDRPVKVEEIEAALSDLRKLTEGGIVGGVSGGG"
     gene            6934..7455
                     /locus_tag="VFQ23_03375"
     CDS             6934..7455
                     /locus_tag="VFQ23_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET9905646.1"
                     /translation="MDQLLTLFWVFLKINLLSTSGAASTGLLYNEAVGHFLTEDQFVQ
                     AVGLATLLPGSDALQLAMFVGYTVGGVSGGLVSLFAAILPPTILMLALVTMLHRIQRE
                     AWLSRFVEGLTPAVAVLIITVAWKVLNSGTGGDLSWIVMAMGGLSLIAYLYEVPAPLV
                     LLVSGLIGTFLFR"
     gene            7452..8003
                     /locus_tag="VFQ23_03380"
     CDS             7452..8003
                     /locus_tag="VFQ23_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET9905647.1"
                     /translation="MNEPTKLQVWFRLFWIFLKVNLLTTAGPTSIGLLYKETVGKIMT
                     EARFVEAVGFSNLVPGSEALKLAMFIGHSAGGIPGVLAALLGAIIPPTVIMLTVASIL
                     VRFEGQDWMKHFIKGMTPAIAVLLALVSWQLFRAANPVSIKWRPIALAILSFIALYLG
                     VSPQYVLIGAGIFGVILYRQWST"
     gene            8000..8848
                     /locus_tag="VFQ23_03385"
     CDS             8000..8848
                     /locus_tag="VFQ23_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9905648.1"
                     /translation="MMNSEFLIVTNGFKGTWPAIEYGAWLAAAMKVKVTLLGVTENQN
                     FAAIDDHGPLEEIFQRAVGLFQQTGVEHTLEVQNGDAERIVTEKANRGDFITIVSRLG
                     RPQLRRWLTGRSIRHLMESIQGPILYVPEARLPLKKILISIGGIGYEVAAENLAFDFA
                     KAIGAEVTIMHVVPPTNLDYPSTRDIREHVNDLAQTNTLPGRGLRTALEIAQQEGLKA
                     NVTARQGQIVEEILAEVKEGNYDMVCMGSPYSASGLRQFYGPNVTAEIAEAVHRPVLI
                     ARYKRE"
     gene            complement(8882..9607)
                     /locus_tag="VFQ23_03390"
     CDS             complement(8882..9607)
                     /locus_tag="VFQ23_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905649.1"
                     /translation="MVEMTYLAIFGIAMAAMFFGYGFGLFEGRGQGYKKRKTEEDQEK
                     KETPEPASSPATITVDDPGLMRIKNDNGSLTLDLDGTRVNASSLSNDQRKRLIEMLNF
                     IRPWLENKPAPAPPSPSASVPVVQQATPVASVPATATPRPISQPVAQNQDKSSTAKND
                     RPAAPANSIVGQIDSILQERLVGTPLEERGVFLAQSPEGGVNVYIGLTRYSSVDEVPD
                     MEIKAAIRAAISEWEDKYTPGLK"
     gene            complement(9600..10412)
                     /gene="mutM"
                     /locus_tag="VFQ23_03395"
     CDS             complement(9600..10412)
                     /gene="mutM"
                     /locus_tag="VFQ23_03395"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET9905650.1"
                     /translation="MPELPEVETIARKLSPHLIGKTILDAQLLWSRTLATPSPKLFKR
                     QVKGQKIWGVTRRAKFFILQLSSSNLLIHLRMSGDLLIRDSTIRPEKHDRLVLQLSDN
                     QSLVFNDTRKFGRVWLTPTPEAILGKLGPEPLSRDFTAQWLYNGLSQRHRQLKPLLLD
                     QTFLAGLGNIYTDEALHIAKIHPLVLSDSVTLEQARGLREAVRKVLKEGIRRHGASID
                     WVYRGGEFQNHFRVYDREGKPCPVCSTEIKRITVGQRGTHYCPNCQAEVIHG"
     gene            complement(10482..11474)
                     /locus_tag="VFQ23_03400"
     CDS             complement(10482..11474)
                     /locus_tag="VFQ23_03400"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET9905651.1"
                     /translation="MSQHLHHDMKMYGEIKLYAGSGSPELAQKIADYLGQQISPQEVI
                     QFPNENIFIKLKSSARGQDVYIIQTTSSPVHYNLMELLIMIQTIRLDSAARITAVVPY
                     LCYGRSDKKDQPRVPITARLVADIIEIAGADRYMTFDPHAGQIQGFFSIPGDVLTASH
                     MVSDYIKKNLLGQMKDPVVVATDLGFAKKGRNYALDMDLPIAFIEKRRIGNDAKAEAL
                     SLIGDVNDRDVIIVDDEVDTGGSIAQAVKVVKEHHGRDIYLAFIHPLFSRDAAKRLAE
                     LPIKQIITTDTVSIPPDKMEPLEGRITILSISSMLGEVIRRAHEGRSVGEMFNE"
     gene            complement(11484..12509)
                     /gene="meaB"
                     /locus_tag="VFQ23_03405"
     CDS             complement(11484..12509)
                     /gene="meaB"
                     /locus_tag="VFQ23_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET9905652.1"
                     /translation="MTKSVLERNRLSISRLLTQVENDSAEGRLTLAELFPHTGNAHLI
                     GVTGAPGTGKSSLVNQLALHYRKNKNHRVAIIAVDPSSPFTGGAVLGDRVRMRDLSGD
                     EGVFIRSMASRGSLGGLAQTTASFTQVFDAAGFEIVIIETVGAGQSEVDIARLAHTTV
                     VVEAPGLGDEIQAIKAGILEIADVLVINKADRPGVELTERALRSTLELAHPTKRVVQH
                     HGRTMAVTQSNAESSVWIPPINKTVATEGKGIAELAESIARHVDHLRQSGDWTARVRT
                     KVGSEVDTLLREELMSRFLENVSREKYDEIIEKVLNRNLSPYEAVQVLMDFASLAVTG
                     EQQGEGK"
     gene            complement(12559..12954)
                     /locus_tag="VFQ23_03410"
     CDS             complement(12559..12954)
                     /locus_tag="VFQ23_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET9905653.1"
                     /translation="MTEKKIRVLVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     PEMIAEAALQEDVDVVGLSILSGAHMALAPRIMELLKANGQENVKVFIGGIVPDEDMP
                     RLKEIGITGIYGPGASTDDIIKDVREAVK"
     gene            13107..13346
                     /locus_tag="VFQ23_03415"
     CDS             13107..13346
                     /locus_tag="VFQ23_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905654.1"
                     /translation="MQARSEWSAWAESLRRLKLDGFAAWALEAAGPLTILGAQAVYIS
                     QPFFGGKQLNTLAQMLEEDEEAQAFARYLRGEESI"
     gene            13343..14077
                     /locus_tag="VFQ23_03420"
     CDS             13343..14077
                     /locus_tag="VFQ23_03420"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HET9905655.1"
                     /translation="MIALTLLVLASVLLIGLTLWRRRSPATLRAIEAYERLNKAVGIA
                     VENGTRLHISLGRGNLFTARGGSALAGLSMLRRLTERTSVSDRPPIVTSGDASLTVLS
                     QDTLQSGYRAASAQDQYRFTTGRLTGLSPFSYAAGTMPIIRDENVSTNIFIGDFGNEA
                     ALLTEEADRQNTNLIAASDNLSAQAIFYAAAQDPLIGEELFAAGAYVGAGASHEASLN
                     AQDILRWLIILAIIIGAGLTVLGAPI"
     gene            14077..14688
                     /locus_tag="VFQ23_03425"
     CDS             14077..14688
                     /locus_tag="VFQ23_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905656.1"
                     /translation="MRVFTAVFAIASGVIVLLGYFFPIAQLDQLRLQLIDWAIIIAGM
                     AVLIGIYNLVAVQMEKIRSGQKGSFYGVLLILSLGITFALGFLLGPNDPFVRLAVDAV
                     IVPVEASLMAILAVTLIYASIRLFRRRVDVTTVLFLLTAVLFLLAIMPTPFGPIPGDW
                     IITQFGGMVSRGGARGLLLGIALGTLLTGLRVLFGIDRPYGGN"
     gene            14691..17792
                     /locus_tag="VFQ23_03430"
     CDS             14691..17792
                     /locus_tag="VFQ23_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905657.1"
                     /translation="MADILCPNCNRNNPDFLDVCQFCQTPLKPEAMVHTGESPTKKHT
                     GELEPILPDWLRDIRQQSKVSTDEDNAKAAAFPSKVEKNEPPDLLAGLASQSGSADED
                     EVPDWLANLNPVIKPTPSAQPSASEPETDFFAQFNKSKPQAADSVEETSKANTPSGTS
                     AEKDELSEWFSQASEQPAETVPVERDDSRIEMGWMDNFDSPASSAQEAPKEAEDLSWL
                     RDLEASAKGTGELSTSKPNIPEAPQEDLSWLNELGGGSIPAQPAAPKEDLSWLDNLGG
                     TSQPSTPPPASEDLSWLRDSGKTAEPSQPTSSAPKEDLDWLNNLGGPSEPQSTTLPET
                     TSPQEDLSWLNNLGGTPVPSQAAEPREDLSWLNDLGESGSSTPAQASVPQEDLSWLKD
                     LGGTSEELTSATEEASKPQEDFGWLKDLGETQEPSSPAPTPPLAPQEDLSWLNNLGGV
                     PESPAQPAASQEENLNWLNELPGKTEAQSADIFQASPTSSDEPDWLKNMGAQSENVPA
                     DTDADAISPRHTAPLSGEAEDSMPDWLKSATEEPSMPLGVGALDKFRDNVPPAESEQP
                     ADDPFAWMDAAQESQQQEEPLPSAPLPATIDANLFAPSTDSSLSNQDVDSLLSMDMPD
                     WLSRPEPAASESSPQETAIPSSMAEGVDETLAPVELPSWVQAMRPVESMAETTPSIDD
                     QPTEREGPLAGLRGLIPIAPIGSARRPKPISLKLQATDEQQAGAALLEQILAGETSPR
                     TIVTSSVVMAQNVLRWALSGLMVLVLGAMIFLRTQTMPINTPLTGDATDLAVRAVANI
                     PENSSVLVVVDYEASLAGEMEAISGPLLDQIALLRHPRLSFLSTSPNGSALAERLLTN
                     TNISRPSPAGLGYNAGEHYFNLGYLPGGGAGVLEFIQAPQIAMPSSPVAQLSEYAAII
                     VLTDHAESGRVWVEQLHSLRQTDPLFPALTSQPLLMVSSAQAGPLLQPYVASGQITGM
                     INGLSDAAKYEFVNSSRPGTARTYWDTFGVGLMLAIISITLGSLWSLFTGIRARRAET
                     EQG"
     gene            17794..18474
                     /locus_tag="VFQ23_03435"
     CDS             17794..18474
                     /locus_tag="VFQ23_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905658.1"
                     /translation="MPFELISAVVGLVLTLMIFSYLVGDNVLFRMAVYIFIGVSSGYA
                     ATIVWHQVLVPKLFAPLQTTNPNQLLLVLVPLVLCLSLLAKLLPRISWIGNVAMAIVV
                     GVGAAAAVGGALLGTLIPQARAAMTAFDVSSAGSGAQAALGLLEGVVMLAGTTFTLAS
                     FHYSASRAADGTVKRNRILESIAWVGRIFVSVTLGVLFAGVYMSALTAMIERLSAIIN
                     FLRGFIGL"
     gene            18478..20235
                     /locus_tag="VFQ23_03440"
     CDS             18478..20235
                     /locus_tag="VFQ23_03440"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HET9905659.1"
                     /translation="MPASLVQNESILAIDVGAAVTRAVMFDVVEGSYRFVASGQAPST
                     AEAPFKDVGIGVREAILNLQNVMGTTLLGPQDNNLIAPSQPDGSGVDAVVATVSAGPT
                     VKAVVVGLLSDVSVQSARRLAESTYSRIVETLDLSDHRRPDQQMDSIVRSRPDLVILA
                     GGTDGGASRSIQKMLEAVGLACYLMPMEKRPMILYAGNRQLANDVQEMLGGHAGKLQI
                     SYNVRPSLETEDLAPASYELASLVMKLRQRQLKGIDELNIWAAGNVLPTAYARGRMIR
                     FLSKLYESTRGLLSVNVGAAATSVAAGFGGELTLGVYPQFGLGENLAGILQYTEIDDI
                     LRWLQLDIAPNTLREYLFQKSIYPSAIPATVDEQAIMQAIARQALYLSVRTAQKDFPT
                     GAHAARTGLMPPLDLILGGGGILSEGTSLGQSLLLLLDGIQPVGIMPVLLDQNNLLPL
                     LGAASSRNHYVPVQVIESGAFIGLGTVVSVIASANYGDQILRAKLTYADGTEVRADVK
                     FGGLEILPLATGESARLSLQPLRRADAGLGSGKSGTVTVTGGALGVIIDARGRPLQFP
                     ADSVRRRELIKKWHYTVGG"
     gene            20237..21292
                     /locus_tag="VFQ23_03445"
     CDS             20237..21292
                     /locus_tag="VFQ23_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905660.1"
                     /translation="MLAPMLHVLPLTTIVRERVLPVAGKVNARVNQRVNPTDVIAEAS
                     FAREHVLLDVAHTFGVSAIAADKMIKVNEGDRLTEGSLVAEKSGIFARTIKAPRPGRV
                     MVAGGGQVLMEVGDARIELRAGLPGMVTQVIPERGVVIRTAGALVQGVWGNGRIDNGL
                     MVSLLEKPDDVLTASQLDVSLRGSVILGGHVKDVETLKAAAELPVRGLVISSLLSSLI
                     VPAYQMRYPILVLDGFGTMPVNSAAFKLLTTNNKREATVNAEHFDRYSGNRPEVIIPL
                     PISTEPDEPNSYEAFTVGQTIRMRRPPHAGMIGSISNLRPGLSLLPSGLRARAADVKL
                     ENGETVLVPLVNLEVVG"
     gene            21411..21950
                     /locus_tag="VFQ23_03450"
     CDS             21411..21950
                     /locus_tag="VFQ23_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905661.1"
                     /translation="MSAYDNDNMNFDDANPPEESSNRTFLLIAGVLGGLVLLALLCLV
                     AYFFFSNNTNQQAELTAQAQATQQAATIQAGLTQTASAGGLTQTAAVTNTVPPTNTPV
                     IAQPTNTFTPTSNPATATVGAAFTQIAVSTQTIIATSTALPNTGFVDDFGGPWWFMTA
                     IALVVVIFLVRRLRAAPTR"
     gene            complement(22058..>22672)
                     /locus_tag="VFQ23_03455"
     CDS             complement(22058..>22672)
                     /locus_tag="VFQ23_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905662.1"
                     /translation="RLGDALLDTINLSAGGSTRVNYPVDNGPIRIVCTTCTNTGTDKI
                     IAALRVIWQEPGYRSSYSELMGLPKEQLSTQYLFPWYNNATQSMDQGFRVANLSLSQS
                     NTVEIWVGGTRLATLSLAAGAATRVGYAVDQGPVRIVCTTCTNIDYDQILVALRVIWK
                     ELGYRTSYTEMMGLPIHAVSTEYWFPRYINSTTIVDQGLRIAVP"
BASE COUNT         5457 a         5797 c         5703 g         5715 t
ORIGIN      
        1 gttttcggct tcaagcgctg tccccgtaat ttcattcaaa agttcaagaa actttgtgtg
       61 cgtctggtta ttctcttcca atgcctccag ctttgccaag gcttcacttc tttcacgagt
      121 gagagaggca agcctgccaa caacaatcgc agtaataatc agggcaccag aaccaataat
      181 ggtgctgggg gtggtgagta attctgaata tgaaatccct gccaatgcct gcacgatgat
      241 gtttacagca atggagaata gggccgtcag gattccaccg gcaatgccaa aataccagct
      301 tgcactaata atcggaacaa tcgctgagga tgcgattaca tcccccacct gttcatgata
      361 caacacgaaa actgctacat aggcagcgta gatggcaaca acgataatgt aggctgaaag
      421 tttgtgcggc gaatgcgcaa gcttattcat tgcaccggtt ggcgataaaa tcgattttca
      481 ctctgcttct tattttatcc ttctggcagc aactatttaa ttacaaattt gagacattcc
      541 ttaaccagga ggaatgtcca gtgaaaatca cttatgtgaa aaacgtgatt tctaccctac
      601 atgattcgga ttcataatat attgccacca gttatgatga atcccatttc tgcgcccggc
      661 agttttcgga aagtggctta gccaccattt gtgatgttcg cggatatccc catcgcccca
      721 gtcggctgca gttacctcac gcgtggtccc tttgaagttt gggaagttat aaagccaatc
      781 gtcacattca ctgacgacag ttgacggatt gttccaatcg tagtcgcggt cactatttgg
      841 ggcgaaatgg acgttgccca cagccgctcg acccggatgt gttttttcat agcgtgtgaa
      901 acgtttccac aaattgacat cctcttccag gttttcaaac gttttttcca taatggattc
      961 ggcgcgatga cccatgttct cgagcatttc gccgacgccg cgctcatagg aaaaccccat
     1021 aatgacaaat cgacgaagcg cagactcagt atttcgcagc ggcgaagcgt tacaccagaa
     1081 tgctcccgga ccgcccataa tcgattcata aaaccctgca tgggggaaat taaacaccca
     1141 cacttcgtca atttcaccag ttgccacgct ttcgagaaca ttaaagcgct cgataatcgc
     1201 gtgataatct gcttcctgcg gcatataggg gcgagtttcg ccgcgcatga ccttaaggta
     1261 ggtatctgca tcgtactgat atccatttac tttcgcgggg aaggcatcca catcgatacg
     1321 ctgaacgatc tgataacgcg ccaggccgct actcacctgc aggatatctg acatatatcc
     1381 tttcgcgagt tcctccacgc tattccagtg catgaactgg gaaagtttgg ttcccgtgga
     1441 ggcttcaacg accgggtcat acacaatgac cagcgtcttg agaacggtta cctgcggtgg
     1501 ttcatcggtc gaatcattga caggtgctgg ctgaatcttc ggcggaggtg aaggtttttt
     1561 tggggaagag aaaaaagatt taagaaagtc cacagtgttt tttgaaccaa tctcgtttca
     1621 gtgactctcc actagggaag attcgtggcg aagtaaacct gattcccaat tgccagatac
     1681 aacacataat tggggctaac tgccatagca cccacgagcc cactctggaa gggattcgcc
     1741 ttcccggcgt gtgcgccaac ctggttctgc aattcgagcg agcgtggcgt gaaacgatac
     1801 acactttgat gttcggaatc cagcaagaga acaaccgagt tgggtgggtt tgtcagcgcg
     1861 atttgcgtaa agtgtgcctg agtgaaaaga tccagtccca ggtgagcggg gaaggaatca
     1921 ttgtaagtag cggggtctac acaacgcgta ggcgttgcgt caattcggct aaaggtacag
     1981 gtggataagt gcccatctgc atgaagcagg tacaggtcgt caccgctcac tgccagatca
     2041 atcgcattgt cgatgttggg aatctgatta ctaaaataga agtaaggtgt atcaataaag
     2101 gtactatctt taccgacaaa gacccacacc gagcgtgagg tggcatcgag aacatataag
     2161 ctgccgttat ccagaacgaa ggctgtgatc cgtccccaat tcgtatttgg caaattaggg
     2221 agaggaatcg cctgcggcac ctgacccggc gagcaataca acaggttccc gttggtatct
     2281 atgccaagga cagttgctcc aatcgcattg actttcggca acgcaagcag gtcaaccatg
     2341 gtacccacct gatatcctgc atgggtccct ggctggcaat taaaggtagt atccaggttg
     2401 agacttcgcc cggtaaagga ggcatgcagg atgttgccgc gctcggcatc caacatatat
     2461 aaatcacttt cactcgccgc catgcggcta atctgtgtcg tatttccaag cccgccagtg
     2521 aatgcagggc tgaattcaag ccgcaccact cccatcagat tatccagctt tgcctgtgcc
     2581 tcggtgcgta acaatctaga ttcatcagtc tcgcgataag catttgcctg atcgagcaaa
     2641 ctcaacacgc gcagccattc gtcccgttgg cgaacaggtt cggcagcgct ttctgcctgt
     2701 gcccgcgcgt tgagtgcctg cccatataac tcctcgtatt gaatgctttg cccgaagcgt
     2761 aaatagactg cacttgctgc agtcactacc agcaccggaa tgacgactgc aatgaagatc
     2821 atgatgtagg ttgggatgac cagcggcttt ttggaactca tcgtcggaag caggcgtggt
     2881 acgaacttat tgatgtatgc tgcgatgcgc tcgttcacgc gccgcccgga tcgaatccca
     2941 cccaccatgg ctttcgccat ctgacgcgcc gcttctgcat gcgcactggg cttgtctgat
     3001 ttcaactgaa ttgaatcgga ctcgtccaag tcttgcgaag ccgcttcgac gttcggtttc
     3061 gaaggctgcg attctgttct tgggatggag gatggaaagg cacgcgagac aggaaccgcg
     3121 gaggcggttg taggttcttc cgtaggtaaa ggaattgttg cttcgggctg tggaggaatt
     3181 gcataggccg atggctggac catatgtgcg gcgaaatctg ctattgcatc cagttcttcc
     3241 tccgtgatcg ggggttcgtc caccggggcc ggttcttccc ctcgaagata gggggaacgg
     3301 gggaaaataa attcttcatg cttgttttgg actttgggct cttcaggtct ttgcagttcg
     3361 gattcctgag gaagcggctg gtctttaacc ggctcaggcc tttccacatg acgatgcaaa
     3421 gcgttcaagt ctggcctgat tactgagatc gtccccaacc cgctttgagc ctggatcaaa
     3481 gcggcgtgca ggtcaccttt ggtaaatgtc agtttggggt agccggcatc cagtgaggcg
     3541 ggtgggcgtt cattgagcag atctgcttcc caatctctgg ggaatttccc gcacaacacg
     3601 atcaaatcat gtggatgcag ttcgacctgc gaaagatagg tttgaatgct ttgactcgac
     3661 ccaaggccct tccccgaaag cgcgggttcg tagatatgtc gcgacttgcc ctcgctgacc
     3721 cagacggcat gtgtttgtcc gctgagcaat aaagtacatt gattttcgcg caccgaagcc
     3781 agtgccagat aagctaatgc atattggcca cgacccgtgg tagataaatt gcgttcgagt
     3841 aaaacagtat taatatgctc ggctgccttg cgcatggcag aagtcaagga gcccgacgac
     3901 tgataaaaga ttccggcagc ctgagagttc agctgcatca gctcgggcgg cagaaatgtg
     3961 gcattccccg tcagcatgat atacaaaatc aggctgtctt tctcgcggcc acgcgcagtc
     4021 ctgcgcggtg gtgtaacagc caacaagcca ggcagtgtct ctgactcctg tccgttgatt
     4081 ctataaatgg ggagaatgtt tagatcgaat gtcgccacag cgtaagaagg ctcaaaatgt
     4141 ttgcatcatt atactggtaa aatttgaacg ttacacacat cagataagga ttagccacgg
     4201 tcccctaacg gggataacaa agacgcggag tcactgagtt ttatttattc tccgcgtctt
     4261 tgctaagcat actcgaagag tgccgtgact cagtggcaaa atgaatttta aattacataa
     4321 agacttctcc gaaattgatg catctacctg gaatgagctc gtgcagcaaa gcatcgccga
     4381 tacgccattc tcacgtcatg aatatctcag cgagtggtgg cagactctcg gcggcggcga
     4441 atggagacag gccgaactgg ttttggtttc ggcgagagac aatgaccagt tgatcggcat
     4501 cgctccatta tttgtcgcgg aatacgaagg ccggcaggcc ctattgctcg ttggtagcat
     4561 tgaaatagca gattatctgg atttgatcgt ccgcgaaccg gatttatcac gtttcttatc
     4621 cgcactgttt gattttctcg atacgtcttc accttcacaa tggtcagcaa ttgactggta
     4681 caacattccc gactcctctc cgactctcgc cgccctcaaa gccgaatctg aaaaacgagg
     4741 ttgggcacac cacgaagaga tttatcgccc tacaccgcgc attccgttga acggatcgtt
     4801 tgaggaatat ctctcacgta tcgataagaa acagcgtcat gagatccgcc gcaaaatgcg
     4861 ccgagccgca gagtctgatc taaacgtccg ctttgtgatc attgataaaa acgcggatat
     4921 tgaatcagag atcgagactt tctttcacct catgatccaa gataccaata aggcaaactt
     4981 cctcacacct ccgatgcgcg aacaaatgac agtgacgatc cgcaccgcat ttgagcagga
     5041 ctatctctgg ttggcatttc tagaggtcga cggaacaaag gtcgccgcct cgctgaactt
     5101 cgattacaaa aataaactat ggggctacaa ctcaggagtc agccgcgagc acatggaact
     5161 atcccccggc tgggtcttgc ttgcacacac cattcaatgg tgttgcgaaa acggacgcta
     5221 tgagttcgat ttcatgcgcg gcgatgagga atataaatat cgctttggcg gggtaaatcg
     5281 gtatgtgatg agggagaggg cgattaaatc gtaattatcc tcccccactg actccgccta
     5341 caattccacc ttctgtcaac ttgcgtaagt cactgagcgc ggcttcgatt tcttccacct
     5401 ttactgggcg gtcttcatcg cgatgtttga gcatacctga gttggctttt tcaatggcca
     5461 gctctcgaat ggaagcgttg tttgccagcg cttccttcac cagtgcggcc ccctgtgaat
     5521 atccgatcac gggattcaat gcagtcacga cgatcgcgtt cttcgccagc cagccttccg
     5581 ctttttcgcg gttggcagta agtcctttga ctgcacgttc ggtaaacgcg ttgactgagc
     5641 caatcactac ctgcatcatc tcaaacaggt tatgcccaag gatgggcatc attacgttga
     5701 gttccaactg tccggcctgc gcggcgagtg cgacggtggt gtcacagccg atcacatgaa
     5761 acatggcttg atcgagcatt tcagccatta ccgggttcac cttgcccggc atgatggatg
     5821 atccaggctg aacggcaggt aagcgaattt cgtcgaaccc ggtcgaaggg cctgatgcaa
     5881 gcagacgaaa atcatttgca atgcggacga ggtccaaagc cagagtccgc aacgacccgg
     5941 agaaatctgc agcgtccccc atggattgca tcgactcaaa taagttatcc gattcataca
     6001 attcaagccc ggtaatctca gataatttct tgaccatacg cgcatgatac tcgggatgcg
     6061 cgttcaaccc agacccgacc gctgtcccac cgattcccag ccgcctcagt ccttctgctg
     6121 acctgcgcac gcgttcgatg ttgcgttcca ccgctttggc gtatgctgaa aattcctgtc
     6181 ccaatgtcac aggcacagca tcctgcaaat gtgttcgccc ggatttgacc acaatctcaa
     6241 actcacgagc ttttgcttcg agcgcgacct gtaaattctt caaggattcc aataattcat
     6301 ccagccgcca caacgcgccc agacgaatcg atgtgggaat cgtatcgttg gtagactgtg
     6361 ccatgttgac atgatcattg ggatggatgt aatactttcc caattcgccg cccaatatct
     6421 gtgtggcgcg attcgcaatg acctcgttag cgttcatatt gtgactcgtc cccgcgcccg
     6481 cctggaatgg ttcaaccaca aattgatcgt cccatttgcc ttccataact tcctcagcag
     6541 cctgggctac ggactccgcc atttgtctgg cagtgaattg ttttccatcc acttcgcgat
     6601 cgttaaacag ccccaattcg taatttacca gcgcggcagc gcgtttcacc gcggcaatcg
     6661 accagacaaa cgcccgccag ggtcttaagc ccgtaatcgg aaagttttct acagcacgtt
     6721 gagtctgcgc gccatacaac gcagcagcgg gaaccggcat ttcacctaat gaatcttttt
     6781 cgatacgaaa ttcgttggaa gtcatgtgat attcaccacg gatcggattt tttatatttt
     6841 acacccttgt aacttgacta tgatatcttc gtctttaatg ggaacactca aattttgacg
     6901 ttacaattgg ggcttatata ttaaggatga accgtggatc aacttctcac acttttctgg
     6961 gtatttctca aaattaatct gctcagcaca tcgggtgcag cttcgaccgg gcttctttat
     7021 aatgaagcgg ttgggcactt tctgacagag gatcaatttg tgcaggccgt ggggctcgcc
     7081 actcttcttc cgggcagcga tgcgctgcag ctcgccatgt ttgtcggcta cacagtcgga
     7141 ggagtctccg gcgggttggt ttctctgttt gcagcgatcc ttccgccaac aatcttgatg
     7201 ctggcactgg tcaccatgct tcaccgcatt caacgcgagg cctggttaag tcggttcgtg
     7261 gaagggctga cgcctgctgt tgcagtatta attatcaccg tagcatggaa agtattgaat
     7321 agcggcacag gaggagacct gagctggatc gttatggcaa tggggggctt aagcctgatt
     7381 gcttacttat atgaagtgcc tgcgccactc gtacttctgg taagtgggct cattgggacg
     7441 tttctgtttc gatgaacgaa cctaccaagc tacaagtttg gttccggctg ttctggattt
     7501 ttctaaaagt aaatctttta acaacagcgg gacccacttc cattggcctt ttatataaag
     7561 agactgtcgg caagatcatg actgaggcgc gatttgtaga agccgtcggc ttttcgaatc
     7621 ttgtccctgg gagcgaagcg ctgaaacttg ctatgttcat tggacattca gcaggaggca
     7681 tcccgggagt gttggctgcc ctattaggcg caattattcc tcctaccgtc atcatgctga
     7741 ccgtggcatc gattttggtc cgctttgaag gacaggattg gatgaagcac tttatcaagg
     7801 gtatgacccc agccatcgcg gttttgctgg ctttggtttc ctggcagctg ttccgggctg
     7861 ccaacccggt atcgataaaa tggcgtccta tcgccctggc aattctaagt tttattgcgt
     7921 tatatcttgg agtttctcca caatatgtat tgatcggcgc gggcattttc ggcgtcatac
     7981 tctatcggca atggagcaca tgatgaattc tgaattcttg atcgtcacga acggattcaa
     8041 ggggacctgg cccgcgattg aatatggtgc ctggctagcg gcggctatga aagtgaaagt
     8101 gacacttctt ggcgtgacgg agaatcagaa cttcgctgct attgatgatc atggcccctt
     8161 ggaagagatt ttccagcgtg cggttggatt atttcaacaa actggggttg aacatactct
     8221 tgaagttcaa aatggcgatg cagaacgcat cgtcacagaa aaagcaaatc gcggcgattt
     8281 catcacaatc gtgagtcggt taggacgccc gcagcttcga cgatggctta cgggtcgatc
     8341 gattcgtcac ttgatggaga gcattcaggg accgatcctg tatgttcccg aagcacgact
     8401 gcccttgaaa aaaatattga tcagcattgg cggtatcggc tatgaagtgg cagccgagaa
     8461 tctggctttt gatttcgcca aggcaatcgg cgcggaggtg acgatcatgc acgtggttcc
     8521 cccgacgaac ctcgattacc caagcacacg tgatatacgt gaacatgtca atgatctggc
     8581 tcagacgaat acgctacccg ggcgcggctt gcggaccgcg ttggagatcg ctcagcagga
     8641 agggctgaaa gcaaatgtca ctgcacgcca ggggcagatt gtggaggaaa ttctggctga
     8701 ggtcaaagag ggaaattacg atatggtctg catgggttcc ccttacagtg caagcggact
     8761 acgtcagttt tatggtccca atgtgaccgc cgagatcgcg gaagccgtgc atcgccccgt
     8821 attaattgcg cgttacaagc gggaataatt tttatactac tggtatagga cgatcacaga
     8881 actactttaa tccgggggta tatttgtctt cccattccga gatcgctgct cggatggccg
     8941 ctttgatctc catgtctgga acctcatcga cgctgctgta acgagttagg cctatataga
     9001 catttacgcc gccttctggc gattgtgcca aaaagacacc gcgctcctcc agcggcgtgc
     9061 cgaccaggcg ttcctgcagg attgagtcga tctgcccaac aatactattc gctggcgctg
     9121 caggtcggtc atttttggca gttgacgact tatcttgatt ttgagcaaca ggctgtgaaa
     9181 tcggtctggg tgtagctgta gccggcacgg aggcaacagg agtcgcctgc tgcacaactg
     9241 gaacagatgc ggatggagaa gggggcgcgg gagcgggttt attttccagc cagggacgga
     9301 tgaaattcag catctcgatc aatcgtttcc gctgatcatt tgaaagagac gaagcattga
     9361 cgcgcgtccc atcgagatcc agagtgaggc ttccattatc atttttgata cgcataagac
     9421 caggatcatc cactgtgata gtggcagggc tggaggcagg ctcgggcgtt tctttctttt
     9481 cctggtcctc ttccgtcttg cgctttttat acccctgacc gcgtccttca aataaaccaa
     9541 agccgtagcc aaagaacatc gcagccatcg cgatgccgaa aattgcaaga tatgtcatct
     9601 caaccatgga ttacctctgc ttgacaattc gggcagtaat gtgtgccgcg ttgaccaaca
     9661 gtaatacgtt tgatctccgt actgcatacc gggcatggct ttccttcacg atcatagacc
     9721 cggaaatgat tctggaattc accgccgcga tagacccagt ctatggatgc gccatgtcgg
     9781 cggatacctt ccttcaacac tttgcgaacc gcttcgcgca aacctctcgc ttgttccaaa
     9841 gtcacagaat cagacaagac tagtggatgt atttttgcga tgtgcaaggc ttcatcggtg
     9901 tagatgttac cgagcccggc aagaaaagtc tgatcaagta acaagggctt aagctggcgg
     9961 tgcctctggc tgagtccatt gtataaccac tgcgccgtga aatctctgct caggggctca
    10021 ggacccaatt taccgagtat tgcttcagga gttggggtta accaaacccg tccaaatttt
    10081 cgtgtgtcat tgaaaacaag ggattgattg tccgataatt gtagtacaag ccggtcgtgt
    10141 ttttctggtc tgatggtact atctcttatc agcaaatcgc cgctcatgcg caggtggatg
    10201 agcagattcg aactcgaaag ctggagaata aaaaatttgg cgcggcgcgt gactccccag
    10261 attttctgac ccttgacctg ccgcttgaag agtttcggcg aaggtgtggc aagtgtacgc
    10321 gaccacagca gctgggcatc cagaattgtt ttgccgatca ggtgtggact caatttccgg
    10381 gcaatggttt caacttcagg aagttcaggc ataagtaagt aactgttagt ttcaagcgat
    10441 tcgctcttga cgccaaagac gaatggcgaa ccacttatcc tttattcatt gaacatctca
    10501 cccacgctgc gcccttcgtg tgcgcgacgg atgacttcac ctaacatgga tgaaatcgat
    10561 aaaatcgtaa tccgcccttc cagcggctcc attttatcag gcgggatgga aactgtgtca
    10621 gttgtaataa tttgtttgat cggtaattca gccaatcgtt tcgcggcatc acgtgaaaaa
    10681 agtggatgga taaaagcaag atagatatcg cgcccatgat gttcttttac aacctttacg
    10741 gcttgcgcga tcgaacctcc cgtatccact tcatcatcaa caatgatcac atcgcgatca
    10801 ttcacatcac caattagact cagagcttcc gcctttgcat cattgcctat tcgtcttttt
    10861 tcaataaagg cgatcggcag atccatatcc agcgcatagt tacgtccctt ttttgcaaaa
    10921 cccaaatcgg tagcaacaac aaccgggtcc ttcatttgtc caagtaaatt cttttttatg
    10981 taatcactca ccatgtgaga ggccgtgagc acatccccag gaatggagaa aaagccttgt
    11041 atctgcccgg catgaggatc gaaggtcata taacggtctg cgcctgcaat ctcgatgata
    11101 tctgccacca ggcgtgcagt aataggcacg cgtggctgat ccttcttatc ggagcgccca
    11161 taacataagt agggaacgac cgctgtaata cgcgccgccg aatccaaccg tatggtttgg
    11221 atcattatca atagctccat caagttgtaa tgcacgggtg aggaggtggt ctggataata
    11281 tacacatcct gcccccgcgc actgctcttc agcttgataa atatattttc attggggaat
    11341 tgtataacct cttgcgggct gatctgttga cccaaataat ctgcgatctt ctgggccagt
    11401 tcaggcgacc ctgaacccgc atacaacttg atttctccgt acattttcat atcatggtga
    11461 agatgttgtg acatccatac tccttatttt ccctcaccct gttgttcccc cgtcactgcg
    11521 agagaggcaa agtccatcag gacctgcaca gcttcataag gcgataggtt tctatttaat
    11581 accttttcaa ttatttcatc gtatttttct cgtgatacgt tctcaagaaa acgactcatc
    11641 aactcctcac ggagtagtgt gtctacttcg gatcctactt tggtacggac tcgggcagtc
    11701 cagtctccac tctgacgtaa atggtccacg tgcctggcga tggactcagc caactctgca
    11761 atgcctttgc cttctgttgc aactgtctta ttaatcggtg ggatccagac acttgactca
    11821 gcgttagact gtgtaactgc catcgtccgg ccgtgatgtt gcaccacgcg tttcgtcgga
    11881 tgagcaagct ccagggtgga gcgaagtgca cgttccgtaa gttccacacc gggccgatcc
    11941 gctttgttaa tcaccagcac atccgcaatt tctaaaatac ccgccttaat tgcctgaatc
    12001 tcgtcaccca gcccgggtgc ttcgaccaca actgtcgtgt gagcaaggcg tgcaatatcc
    12061 acttcacttt gccctgcgcc caccgtttcg atgatgacga tctcaaaacc tgcggcatcg
    12121 aaaacctgcg taaaggaagc ggttgtttgt gcaagacccc caagtgaacc gcgcgaagcc
    12181 attgaacgaa tgaacacgcc ctcatcgcca gagaggtctc gcatacggac gcgatctcca
    12241 agaaccgcac caccggtaaa tggactggat gggtccactg caataatcgc aactcgatga
    12301 ttcttatttt tccgataatg cagtgccagt tgattgacca gggaagattt ccccgtacca
    12361 ggcgcgccag tgacaccgat aagatgagca ttacctgtat gggggaagag ctccgcgagt
    12421 gtgaggcgcc cttccgccga atcattttcc acctgagtca ataagcgtga tatggagagt
    12481 cgatttcttt caagaactga ttttgtcaac gacatttttc ttgtcacaaa actacgtggt
    12541 gctagggcac cacgtagttt acttcactgc ctcccggacg tctttgataa tatcatccgt
    12601 tgatgcgccg ggaccgtaaa ttcctgtaat gcctatttcc ttgaggcgtg gcatatcttc
    12661 gtcagggacg atgcctccaa taaagacttt tacattttcc tgtccgtttg ccttcaacaa
    12721 ttccatgata cgcggcgcaa gcgccatatg agcacctgaa aggatggaaa gccctaccac
    12781 atccacatct tcctgaagag cagcctccgc gatcatctca ggcgtctggc gcaaacctgt
    12841 gtaaatgact tccatcccgg cgtcacgcaa cgcacgcgcg actactttgg cgccgcgatc
    12901 atgtccatcg agcccaggct tggcaaccag tacacggatt tttttctcag tcatgtattc
    12961 ctcgattagt aatttctatt gtcattatac tatgacgata atctcacaaa aaagatgccg
    13021 ccattcttct caattcgcaa tgacactgac atttccggat tattcatgcg agtcaggtca
    13081 cctttaaaat atgctatact ttactcatgc aagcgcgatc tgaatggtct gcctgggctg
    13141 aatcattgcg tcgcctcaaa ttggatggat ttgccgcctg ggcacttgaa gcagcgggac
    13201 ccttaactat tctgggggct caggcagtgt atatcagtca gccatttttc ggtgggaaac
    13261 aattgaacac tcttgctcaa atgctcgaag aagatgaaga agcacaagcc tttgctcgtt
    13321 atctacgcgg tgaggaatcc atatgatcgc gttgacattg ttagttttag cttcggtcct
    13381 cctgatcggt cttactttat ggcgtcgcag gtcaccagct actttacgcg ccatcgaagc
    13441 ctatgagcgc ctgaacaaag ctgtgggcat tgctgtcgaa aatggaacac gcctgcatat
    13501 ttcactcgga cgtggaaacc tcttcaccgc gcgcggtggc tctgcattgg ctgggctttc
    13561 gatgctccgt agattgaccg aacgaacctc cgtcagcgat cgcccaccca tcgttacatc
    13621 cggtgatgca tcgcttactg ttctttcaca ggatacgttg cagtctggtt atcgggctgc
    13681 atcggcgcag gatcaatatc ggttcacgac cggacgatta accggactct caccattctc
    13741 ctatgctgcc gggacgatgc cgatcatacg tgatgaaaat gtttcaacaa atattttcat
    13801 tggtgatttt ggaaatgaag ctgctctatt aacggaagaa gcagatcgcc aaaatacaaa
    13861 cttgatcgca gccagtgata atctctccgc gcaggctata ttctatgcag cggcacagga
    13921 tccgctgatc ggcgaagagt tgtttgcagc cggagcgtac gttggtgcag gtgcatcgca
    13981 cgaagccagt ttgaatgcac aggatatttt gcgctggttg atcatattgg ccatcatcat
    14041 tggagctgga ctgaccgttt tgggagctcc catctaatgc gcgtatttac cgcggtcttt
    14101 gccatcgctt ccggggtgat cgtcttattg ggctatttct ttccgatagc gcagttggat
    14161 caattacggt tgcaattgat tgactgggca atcatcattg cggggatggc agttctgatc
    14221 ggaatttata atctcgtcgc tgtacaaatg gaaaaaattc gctccgggca aaaaggcagt
    14281 ttctatgggg tgttactgat tttgtcgctg ggaattacat ttgcacttgg gttcttgctg
    14341 ggtccaaatg atccgtttgt gcgcctggca gtagatgcag taatcgtccc ggtggaagcc
    14401 tcactcatgg caatcctggc ggttaccttg atttatgcca gtatccgcct cttccgccga
    14461 cgtgtggatg tgaccactgt attgttttta ttgacggccg ttttgttttt actggcgata
    14521 atgcccactc cgttcggtcc gataccggga gattggatca tcacgcaatt tggaggcatg
    14581 gtttcgcgcg gaggtgcgcg cggtttgttg cttggcattg ccttgggaac cttactgaca
    14641 ggtttacgcg tcctgttcgg gattgaccgc ccgtatggtg ggaattaatc atggctgata
    14701 tcctttgccc caattgcaac aggaataatc ccgatttctt agatgtctgc cagttttgtc
    14761 agactccgct caaaccagag gccatggtac ataccgggga aagccccaca aagaagcata
    14821 cgggcgaact tgaaccaatt ttgccagatt ggctacgaga tatacgtcag caatcgaaag
    14881 tttcaacaga tgaagacaac gcgaaagcag cggcttttcc gtccaaggtc gagaagaatg
    14941 agccgcctga tctcctggca ggtctcgcct ctcaatctgg ttccgcggat gaagatgaag
    15001 tcccagactg gctggcaaat ctaaacccgg tcatcaaacc cacaccttca gctcaaccct
    15061 ccgcgtcaga gcctgaaaca gattttttcg cacagttcaa taagagcaag cctcaagctg
    15121 cagactcagt agaagaaact tcaaaggcaa acactccatc aggaacttct gcagaaaaag
    15181 atgaattatc tgaatggttc tctcaggcct ctgagcaacc cgctgagact gtccctgttg
    15241 aacgcgacga ctctcgaatc gaaatggggt ggatggacaa cttcgactct cctgcaagtt
    15301 cggctcagga agctccgaaa gaggcagaag atttgagctg gcttcgagat ctggaagctt
    15361 cagcaaaagg gactggcgag ttatccactt caaagccaaa tattcctgag gctccacaag
    15421 aagatctcag ttggctaaac gagttgggag gcggttcaat tcctgcacaa cccgccgcgc
    15481 caaaagaaga ccttagctgg ctcgataatc ttggaggaac ttcacaacca tctacacccc
    15541 cacccgcctc ggaagactta agttggttga gggactcggg aaaaacagct gaaccgtcac
    15601 agcccacttc ctctgcacca aaagaggatt tggattggct caataatctt ggcgggccaa
    15661 gcgaacccca atcaacgact ttgcctgaga ctactagccc acaagaagat ttgagttggc
    15721 taaataatct gggaggtacc ccggtacctt cacaagcagc tgagccaaga gaggacttga
    15781 gctggttgaa tgatcttggc gagagcggtt catccacacc tgctcaagcc tctgttccac
    15841 aagaagattt aagttggtta aaggatcttg gcggtacatc agaagaattg acatctgcca
    15901 ccgaagaagc gtcgaaacct caagaagatt ttggttggtt gaaagatcta ggcgaaactc
    15961 aagagccatc atcccccgcg cctactccac cactagcgcc gcaagaagac ctgagttggc
    16021 ttaataatct gggtggagtg ccagagtcgc ctgcccaacc ggcggcttca caggaagaga
    16081 atctcaactg gctgaatgaa ctgcccggca aaactgaagc tcaatctgct gacatttttc
    16141 aagcttctcc aaccagttcc gatgagcccg attggctaaa gaacatggga gcacaatcgg
    16201 agaatgtacc cgctgacact gatgcagatg caatctctcc gcgtcacacg gcgcctctga
    16261 gtggagaagc agaagactcg atgcccgatt ggctgaagag tgcaacagaa gaaccgtcta
    16321 tgcccctggg cgtcggagcg ctggataaat tccgcgacaa tgtcccaccc gccgaatcag
    16381 agcaaccagc cgacgatccg tttgcatgga tggatgctgc tcaggaatca caacagcaag
    16441 aagaaccgct tccttccgcc cccttgccgg ccaccattga tgcgaatctg ttcgcccctt
    16501 caactgactc ctcgctttcg aatcaagatg tagattcgct cctctccatg gatatgccgg
    16561 actggctttc acgtccggaa ccagcggcat ctgagagttc cccgcaggaa acggctatcc
    16621 catcttcgat ggctgaagga gtggatgaga cactcgcacc cgtagaactg ccatcctggg
    16681 ttcaggccat gcggcccgtg gaatccatgg cggagacaac gccaagcata gatgaccaac
    16741 caaccgaacg tgaaggtcca ttggcaggtt tgcgcggtct aataccgatt gcgccgattg
    16801 gttcagcacg acgaccgaaa cccatttcgc taaagcttca ggcaacggat gaacaacagg
    16861 ctggcgcggc tttattggaa cagattcttg ctggggaaac ctccccgcga acgatcgtaa
    16921 catcctccgt agtgatggcg cagaatgtat tgcgctgggc actgagtgga ttgatggtcc
    16981 ttgtgcttgg tgcaatgatt tttctgcgta cccaaacgat gccaattaat acccctttaa
    17041 cgggcgatgc aacggactta gccgttaggg cggtagcgaa cattccagaa aattcatctg
    17101 ttttggttgt cgttgattac gaagcctccc tggctggtga aatggaagcg atcagtggtc
    17161 ccttgttgga tcaaattgcg ctgttgcgtc atccgcgact ttcatttctt tctacctcac
    17221 cgaacggctc tgcgctggca gaacgcttac tgacaaatac aaacattagt cgaccctcac
    17281 ccgccggact tggatacaac gcaggtgaac attacttcaa tttagggtat ttgcccggtg
    17341 gcggagcggg tgtcttggaa tttattcagg ccccgcaaat tgccatgcca tcctctcctg
    17401 tcgcacaact ctctgaatac gctgcgatta tcgtgttgac tgaccatgcg gaatcaggac
    17461 gagtttgggt agagcaactt cacagcttga gacagaccga tccactattc cccgcattga
    17521 ccagccagcc actgctcatg gtctccagcg cccaggctgg accactattg cagccttatg
    17581 ttgcatccgg tcagatcaca ggcatgatca atgggctttc cgatgctgcc aaatatgagt
    17641 ttgtcaatag cagcagacct ggcaccgctc gcacatactg ggacacattc ggtgtgggct
    17701 tgatgcttgc gattatttca ataacacttg gcagcctgtg gagtcttttc acagggatcc
    17761 gtgcgcgacg cgcagagaca gagcagggat aagatgcctt ttgaactaat ctctgcggtg
    17821 gtgggtttgg tccttactct gatgatattt agttatcttg tgggtgataa cgtactgttt
    17881 cgcatggcag tgtatatttt tattggcgtt tcgtcagggt atgcagctac cattgtgtgg
    17941 catcaagttt tggtccccaa attgtttgcg ccgctgcaga ccactaaccc gaatcaattg
    18001 ttattggtgc tggtaccact tgttttatgt ttatccttgc tcgccaaact tcttccgcga
    18061 atttcatgga ttggaaatgt agccatggca atcgtggtag gtgtgggagc ggcggcagct
    18121 gttggtggtg ctctgcttgg cactttgatc ccacaggctc gcgccgcgat gacagcattt
    18181 gacgttagtt ccgcgggcag tggcgcccag gcagcccttg gattgcttga aggagtcgtc
    18241 atgctggcag gcacaacctt tacactggca tccttccact acagtgcaag ccgtgcagca
    18301 gatggcacag taaagaggaa tcgcatcctt gagagcatcg cctgggttgg gcgcattttc
    18361 gtttctgtca cgttaggggt tctgttcgct ggagtttata tgtccgctct tacggcaatg
    18421 atcgagcgcc taagtgcgat cataaatttc cttagaggat tcatcggtct ataaattatg
    18481 cctgcatcgc tggtacaaaa cgaatccatt ttagcaatcg acgttggcgc ggcggtaacg
    18541 cgggcggtta tgttcgatgt ggtagaggga agttatcgct ttgttgcttc cggacaggcc
    18601 ccatcgacgg cagaggcacc gttcaaggac gtaggcatcg gtgtgcgtga agccatcctc
    18661 aatttgcaaa acgtgatggg caccacgtta ctcggtccgc aggataacaa tttgatcgcg
    18721 ccctcacagc cagatggctc cggcgtagat gcagtggttg ccactgtttc cgcagggcca
    18781 acagtaaaag cggttgtggt tggactgcta tccgatgtgt ccgtgcagag cgcgcggcgg
    18841 ttggccgaat ccacgtacag tcgaatcgtg gaaacgcttg atctttcgga tcaccggcga
    18901 cccgatcaac aaatggatag catcgtccgg tcacgccctg atctcgttat attggctggc
    18961 ggcacagatg gcggggcatc acgctccatc caaaagatgc ttgaagccgt tgggcttgcc
    19021 tgttatttga tgccgatgga aaaacgccca atgattttgt atgcagggaa tcggcaactg
    19081 gcaaatgatg ttcaagaaat gcttggtggg cacgcaggga aacttcaaat cagttacaac
    19141 gtgcgcccat cgcttgaaac agaagacctg gcaccagcca gttatgaatt ggcttccctg
    19201 gtgatgaaac ttcgccagag acagctcaaa ggcatcgatg aactaaatat ctgggctgcc
    19261 gggaatgttt tgccaaccgc ttatgcccgc ggacggatga ttcgattctt aagcaagttg
    19321 tatgaatcca cgcgtgggct gctgtctgtc aatgtcggag ccgctgccac ctctgttgct
    19381 gccgggtttg gaggcgaact cacgcttggc gtctacccgc aattcggact tggcgagaat
    19441 ttggctggca tcctgcagta cactgaaatt gacgatatcc tgcgatggct tcagcttgat
    19501 attgcgccga acacactgcg tgaatattta ttccagaaat ctatatatcc ttcggccatt
    19561 cccgcaacag tagatgagca ggccatcatg caggcaattg ctcgtcaggc attgtatctt
    19621 tcagtgcgaa ctgcccaaaa ggatttcccc accggcgctc atgccgcacg aactggactc
    19681 atgccacctt tggatcttat tctcggcggc ggcgggatct tgagcgaagg aacctcactc
    19741 ggacaaagtc tgttgctgct gctcgatgga atccaacccg ttggtatcat gccggttttg
    19801 cttgatcaaa ataatctatt gccgttgctc ggcgccgcct cttcacggaa tcattatgtg
    19861 cccgtgcagg tcatcgagtc cggtgcattt atcggattgg gaacggtggt atctgtgatc
    19921 gcttcagcga attatggtga ccagatcctt cgggcgaagc tcacctatgc cgatggaaca
    19981 gaggtacgag cggatgttaa gtttggtggg ttagaaatcc tgccgcttgc caccggtgaa
    20041 agcgcccgtt tgagcctgca gccacttcgt cgcgcggacg cagggctggg atccggaaaa
    20101 agtggcactg tcaccgtgac aggcggcgcg ttgggagtca tcatcgacgc gcgcggacgt
    20161 ccgcttcagt tccctgccga ctctgtgcgt cgacgtgaat tgatcaagaa atggcactac
    20221 acggtgggag gataaaatgc ttgccccaat gctgcacgtc cttccactaa cgacaatcgt
    20281 acgagagcgt gttctgcctg tagcaggcaa ggtcaatgca cgtgtcaatc aacgagtcaa
    20341 cccgacggat gtaattgccg aagcaagttt tgcacgcgaa catgtgttgt tggatgtagc
    20401 tcacacgttc ggcgtttctg caattgcagc cgacaagatg attaaagtaa acgaaggcga
    20461 ccgactgaca gagggctctc tggtcgcaga aaaaagcggc atattcgccc gcacgatcaa
    20521 ggcgccgcgt ccaggtcgtg tgatggttgc agggggaggt caggtattga tggaagtggg
    20581 cgatgcgcgc atcgaactac gtgccggtct gcccggcatg gtcacgcaag tcatcccgga
    20641 acgtggcgta gtgattcgca ctgctggcgc gcttgtgcag ggagtctggg ggaatggccg
    20701 cattgacaat ggcttaatgg ttagcctgct cgagaagcca gatgatgtcc tcaccgcaag
    20761 tcagttagac gtgagcctgc gtggatccgt catcctgggt ggccacgtga aggatgtcga
    20821 aacgctaaaa gcagctgccg aattacctgt acgagggcta gtcatctcga gcttgctttc
    20881 atcactgatc gttcccgcat atcaaatgcg ctacccgatt ttggtactgg acggatttgg
    20941 aacgatgccg gttaattctg ccgcattcaa attgttgacg accaacaata aacgtgaggc
    21001 aacggtgaac gctgaacact ttgaccgtta tagcggaaac cgacctgaag tgatcatccc
    21061 gttgcccatc tcgacggagc ccgatgagcc aaacagctat gaagccttca ctgtagggca
    21121 aaccattcgc atgaggcgcc caccgcatgc agggatgatc ggctccatct ccaaccttcg
    21181 cccgggattg agtctcttgc ccagtggatt acgcgcacgc gcggcagatg tgaagcttga
    21241 aaatggtgag actgtgctcg tgcctttggt aaatcttgaa gttgtgggat aataaattga
    21301 taacatcact ttacgtgaaa cttcgtctca gagaaaattc aaaaagctcc ataggctctg
    21361 cggctatgtg cgtaaggtgg gtgtaaaagt aacatagagg agaatgtaca atgtccgcat
    21421 atgataatga caatatgaat tttgatgatg caaatcctcc agaggaatcc agcaatcgca
    21481 catttcttct cattgccggt gtccttggcg gacttgtgct tctcgcactg ctctgtcttg
    21541 ttgcatattt cttcttctcg aacaacacca accaacaggc tgaattaact gcacaggcac
    21601 aagctacaca acaagcagcc accatccagg cgggcttaac ccagactgct tcagcaggag
    21661 gtcttacaca gaccgccgcg gtaaccaaca cggtacctcc caccaataca cctgttattg
    21721 ctcaaccaac caataccttc actcccacat cgaatcctgc tactgcgacg gtcggtgcag
    21781 cattcactca aattgcggtt tcgacacaaa ccattatcgc gacctccact gccctgccca
    21841 ataccggctt cgtggacgat ttcggtggac cctggtggtt catgactgct attgcattgg
    21901 tggtagtgat tttccttgtg agaaggttga gagcagcacc aacacgataa tgtgttcgtt
    21961 tagaactgaa tggacctgtg aaaagtaatc acaggtccat tttttatctg atacgaccta
    22021 atgataagat tctgaaatgt actacataaa ataccattta tggcacagct attctaaggc
    22081 cttgatcaac aatagtagtt gaattgatat accgggggaa ccaatactcc gtggagacgg
    22141 cgtggatagg taaccccatc atctccgtat aactggtccg atacccaagc tccttccaga
    22201 ttacacggag cgcaacaaga atttgatcat agtcaatgtt tgtacaagta gtacacacaa
    22261 tccgtaccgg gccctgatct accgcatacc cgacacgcgt ggctgctccg gctgccaggg
    22321 aaagggtcgc cagccttgtg ccacctaccc agatttcgac cgtattgctc tgactgaggg
    22381 agagatttgc gacacggaaa ccctgatcca tcgattgagt ggcgttgttg taccagggaa
    22441 acaaatactg ggtggacagt tgttcttttg gcaaacccat cagctcagag tatgaactgc
    22501 gataaccggg ctcctgccag atgacgcgca aggctgcaat aattttgtca gtgcccgtgt
    22561 tggtgcacgt cgtgcacact atgcgaatcg gaccattatc taccgggtaa ttcactctag
    22621 tgctcccgcc tgctgaaagg ttgatagtat ccaggagtgc atcgcccaac cg
//
LOCUS       DASTJP010000111         6757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_31293, whole genome shotgun sequence.
ACCESSION   DASTJP010000111 DASTJP010000000
VERSION     DASTJP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31293"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFQ23_03460"
     CDS             <1..433
                     /locus_tag="VFQ23_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="arginosuccinate synthase"
                     /protein_id="HET9905663.1"
                     /translation="AVLFGNTLSWAGEYLLRQGPVLPASDGLARKVKPLCRFYEREMT
                     AYCLARGIEYIYEECPFAEGSQSIYYKELLNQLEMTRPGAKLTFYLRFLEARKSGNLF
                     IEPIDQRTHLHPCINCGQPTSAPDLCSFCRTIEKVNSVSAS"
     gene            529..1794
                     /locus_tag="VFQ23_03465"
     CDS             529..1794
                     /locus_tag="VFQ23_03465"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET9905664.1"
                     /translation="MTPISIPKQTARRFVLGKQGLWPGRRWKGKKGTAQAIRACEAVQ
                     LDPLNVVARSQDLVLHSRVLDYKPEYLYDVAYKERQFFDYGGWLAMYPMEELPYWRVH
                     MEKRSRDKRIEGFVLSHDELFDQVRAELRARGPLGNRDLDGNKVGSWNYRGRKDTSLA
                     LFDMWLSGELMMHHRDGFSRVYDFRENIAPKEFDYVATEKEAEAFFARKAVAFVGLKR
                     ESNMRVELNYYLRREYSKAEMMRLIEGWKEAGTFAQVQVQGERETYLILSQDIPLLEM
                     LEKGKIPKAWKPKETTTLEEVTFLASLDIVSARGRAKKLFDFDYVWEVYVPAHKRRWG
                     YYVLPILYGDDLVARLDPKLDRTTGTLEIKGFWHEDDAPVKDPAFAKALANGLVRFAN
                     FVDAKKVTTGAIHPAGLRNQVEKLLRASL"
     gene            1944..2198
                     /locus_tag="VFQ23_03470"
     CDS             1944..2198
                     /locus_tag="VFQ23_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET9905665.1"
                     /translation="MAKLKLDLHDIYNRGKEIDSELNRIVQEAIDKKISLVEIIPGKG
                     SGQLKKKVLRFLNQGHIKKLYHRVEKDDKNFGRIFIHFKH"
     gene            complement(2322..3440)
                     /locus_tag="VFQ23_03475"
     CDS             complement(2322..3440)
                     /locus_tag="VFQ23_03475"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9905666.1"
                     /translation="MTKDIFISYSRRDQEFVTRLASDLDAHVTGVWFDQSTIQLGQKW
                     HDEIMEGIRECKAFILVLSPDAAESKYVREEVNKALELGKTIFPILYRPGNWSGEFAS
                     LVKEIQTLDLRSGSYTDNFQKLVDGLIEAGAVKSTGERPFLRQSTVMPMSAVISKIPG
                     WAFAWSLGWVAFWMIVIIVLIFLLQSQGDMGSDDVVAFIVILIGGGIGGFTGGLLAGL
                     FTMLALRPNAPSISWRHMAPTIRIWAISGPLGMLVSGLVTSLLIAVGTIGMGTAEPDC
                     NGGFGDCVGQIIGTAIGNVLVFAFVVVLVFLLFVTVAWFLTGMFAGGLAVRHIRRLEP
                     GITSGQSRRVSIGWGCGAIVAAIVTLILTGILLNALGL"
     gene            complement(3462..6284)
                     /locus_tag="VFQ23_03480"
     CDS             complement(3462..6284)
                     /locus_tag="VFQ23_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9905667.1"
                     /translation="MTSLFISYSRRDIDAARRLTDAFKGQELDFWIDWEGIPPTVDWW
                     KEIEKGIEEADIFLFLISPDSTKSKICRQEIDHAIKNGKRLIPVVVREIQSDESPEAL
                     SHLNYMFFRETDEFEPAFNKLLIAIKTDFAWAQVHRQLQVKALEWDRSGKEDSFLLHG
                     KELQDAEFQLATNTSKEPHPTDLQREYVFKSRQASDVSRRRTRSIGIGVIIALLVLSA
                     VAWVQAGRATTNAEVAEAASILAVANAGTAQANEQEAQRQADLALARELAASSINKLD
                     LDPELSLHLAILSIDKKQTLQGEEALRRALLSPPVERTLRGHSGTVYSVAYDSDGKRL
                     VTASEDGTARIWDAATGEELIALSSHEGAVKDAAFSPDGRLVATAGADGIVMIWDTVT
                     GEQLRSMKHQSSVNSVAFSPDGQMLVTASDDATARVWNVDTGRQMYVLRGHSAAVLGA
                     AFSPDGKLIATGGNDTLVKIWNAATGEFINEISNGDAPYNEAVADLAFSEDSQYVLTT
                     GGYIPRLLDIQNAFVLTEYLEGHTFYVISGAISPGDGRQVVTASRDHTIRIWDTETAR
                     SIEVLRGHSGTVNEVVFDPTGNHLATASEDQTVRIWNIGEWRKRILIGHTDKLTGAAY
                     SSNGQLIATASEDRTIQIWNAQTGHEQIHWEYPGWLASGVAFSPDDQQLITASGDGTW
                     RIWDVDTAQQLSSTDFITSIVHDARFNKDGTRIIIANRDTFGYILDADTKELQVILEG
                     HTDWVASAMFGDDGKLAVTASADGTARLWNAETGEPLRIFAGHSGLVTRAAFSSDGKR
                     IVTSSVDGTARIWDTATGEVLQTLKGHTAEVTDAAFSPNDDYVATASRDNTARLWNPD
                     TGDEVVQLFGHTDDLRTVAFSQDGRYLLTSSFDDTARIYPVYFDDVLALARSLLSPRE
                     LTCAERIKYLHDEICTIPTLTVTPTP"
     gene            complement(6286..6444)
                     /locus_tag="VFQ23_03485"
     CDS             complement(6286..6444)
                     /locus_tag="VFQ23_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905668.1"
                     /translation="MTKDIFISYSRRDQEFVTRLASDLNAQVAGVFAPFPAFPQIKNG
                     FGGRIMGL"
     gene            complement(6552..>6757)
                     /locus_tag="VFQ23_03490"
     CDS             complement(6552..>6757)
                     /locus_tag="VFQ23_03490"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HET9905669.1"
                     /translation="STTINPRLNGPLYVRGHMRIMGSGGSLIREDTRVALCRCGHSEN
                     KPFCDGSHRKVGFRSGTTSEGSR"
BASE COUNT         1679 a         1738 c         1670 g         1670 t
ORIGIN      
        1 cgcggtttta tttggaaata cgctttcctg ggcaggtgaa tatcttctac ggcagggacc
       61 ggtccttcca gccagtgatg gattggcgcg aaaggtcaaa ccgttgtgcc ggttctacga
      121 acgagagatg acagcatact gcctggcgcg tgggattgag tatatttacg aagagtgtcc
      181 gtttgccgaa ggatcacaat ccatttacta taaggaattg cttaatcaac ttgagatgac
      241 ccgccctggt gccaagctga cgttttattt gcgttttctt gaagctcgca aaagcgggaa
      301 tttatttatc gaaccaattg atcagcgaac ccatttgcat ccctgtatca attgtggaca
      361 acccacctcc gcgccagacc tttgttcatt ctgtaggaca attgagaaag taaactctgt
      421 gtcggcatcg taggattgtt tgtcaaacgt gccttccggg ggagtgttat atttatgctt
      481 agaatatatg tgctaaaata acttccattc ctatttactt taactttcat gacacccatc
      541 agcatcccaa aacaaactgc gcgacgattt gtccttggca aacaggggct atggcctggc
      601 cgccgctgga agggcaaaaa aggaacagct caggcgattc gcgcatgcga agctgttcaa
      661 cttgatccgc tcaatgtcgt tgctcgaagt caggatttgg tgctgcacag ccgggtattg
      721 gattacaaac cagaatatct atatgatgtt gcctataagg agagacagtt cttcgattac
      781 ggtggctggc tggcaatgta tccgatggaa gagttgccct attggcgcgt gcatatggaa
      841 aaacgcagcc gcgacaaacg tattgaaggg ttcgtgctca gccatgatga actcttcgat
      901 caggtgcggg cggaacttcg cgctcgcggt ccgttaggaa atcgagacct cgacggaaac
      961 aaagtcggtt cgtggaatta tcgagggcgc aaagatacat ccctcgcctt gtttgacatg
     1021 tggctttcag gcgagttgat gatgcatcac cgcgacggct tcagccgggt ctatgacttc
     1081 cgcgagaata tcgcaccgaa agagtttgat tatgtggcaa cggaaaaaga agcggaggcg
     1141 ttcttcgcgc gtaaggcggt tgcgtttgtg gggttaaagc gcgaatcgaa tatgcgggtt
     1201 gagttgaatt attatctgcg acgtgagtac tcgaaggcag aaatgatgcg gctgattgag
     1261 gggtggaaag aggcagggac gttcgcgcaa gttcaggtcc agggcgaacg cgaaacctat
     1321 ctgattttgt cacaagatat tccattgctc gaaatgctcg aaaaaggaaa aatcccaaaa
     1381 gcctggaaac ccaaagaaac aacaaccctg gaagaagtca cctttctcgc ttcactggat
     1441 atcgtgagcg cacgcggtcg ggcgaaaaag ctatttgact tcgattacgt ctgggaagtc
     1501 tatgtgcctg cgcacaaacg gcgctgggga tattatgtgc tgccgatctt atatggcgat
     1561 gatctggtag cgcggctcga cccaaaactt gaccgcacga ccgggactct ggagatcaaa
     1621 ggcttctggc acgaagacga tgcacccgtg aaagacccgg cgtttgcgaa ggcactcgcc
     1681 aatggacttg ttcggtttgc aaattttgtg gacgcgaaga aagtcacaac aggcgcaatt
     1741 catcccgccg ggttacgcaa tcaagtggag aaacttctgc gtgcttcact atagattaca
     1801 tttcgtaaaa cccctgcaag atgatcacaa tccgggcttc tctggtttta tggtttacgt
     1861 caatgataga atcgactcag gcgggaggat gcgtttgaaa cgtatactga tgcaggatcg
     1921 tagatcagac agttgaggaa tgaatggcca aactcaaatt ggatttacac gatatttata
     1981 atcgtggtaa ggaaattgat tcagagctga atcgcatcgt gcaggaagcc atcgataaga
     2041 aaatctccct ggttgaaatt atccccggca aagggagcgg acaactcaag aaaaaagttt
     2101 tacgctttct caatcagggg cacattaaaa agctatatca tcgcgtggag aaggacgata
     2161 aaaactttgg gcggattttc atccacttca agcattgatg ttctagaaga gtctaaaatc
     2221 gaaaacaatc agcaatcctc acttaatcgg agatcgcttc aaactacacc tgctttcgcg
     2281 tgctcgttca agtggcgcac tcgccatcac ataaagacaa cctaaagccc caacgcgttt
     2341 aaaagaatcc ctgttaagat gagcgtcacg atcgccgcaa cgatggctcc acatccccag
     2401 ccaatagata ctctccgact ctgaccactt gtaattcctg gttcaagcct gcggatgtgg
     2461 cgtactgcca gcccacctgc gaacattcct gtgaggaacc atgctacagt aacaaagaga
     2521 aggaatacca aaactacaac aaacgcaaat acgagtacat tgccgatcgc tgtgccgatg
     2581 atctgcccaa cgcaatcgcc aaacccacca ttgcaatctg gttcggcggt ccccataccg
     2641 atcgtgccca ccgcaatcaa cagagatgtg accagaccag aaaccaacat cccgagggga
     2701 ccgctgatcg cccaaatgcg gattgtggga gccatatgtc tccacgatat gctgggtgca
     2761 ttgggtcgca aggcaagcat tgtaaagaga cctgcaagca gaccgccagt aaaacctccg
     2821 atcccgccgc caatcagaat cacaataaat gcaactacat catcgcttcc catatccccc
     2881 tggctctgaa gaaggaagat gagaacgatg atgacaatca tccaaaaggc cacccagccg
     2941 aggctccagg caaacgccca accgggaatt ttgctaatga cggcgctcat cggcattacc
     3001 gtggattggc gaagaaacgg tctttctccc gtggatttta cggcgcccgc ttcgatgaga
     3061 ccatctacca gcttttgaaa attatcggtg tagctgcctg agcgcaggtc gagtgtttgg
     3121 atctccttca caagcgaagc aaattcaccc gaccaattcc caggccgata gagaatcggg
     3181 aagatcgttt tcccaagctc taatgcttta ttcacttcct cgcgcacata cttagattcg
     3241 gctgcgtcag gtgaaagtac caatatgaag gctttgcatt cgcgaatgcc ctccataatt
     3301 tcatcgtgcc acttttgccc caactgaatc gtggactgat caaaccacac gcctgtaacg
     3361 tgtgcatcca agtcacttgc cagacgcgtg acaaattcct gatcgcggcg ggaataagag
     3421 atgaatatat ctttggtcat ctgtgaggac acatttttat cttatggcgt cggagtcacg
     3481 gttaatgttg gaattgtaca tatttcatca tgcaaatact ttatacgctc ggcacaggtc
     3541 agttcgcgcg gtgaaagcag cgaacgagct aatgccaaaa catcatcaaa gtatacagga
     3601 taaatccgcg cggtgtcgtc aaagctggat gtaagcagat aacgaccatc ctggctgaat
     3661 gccaccgtac gaaggtcgtc ggtgtggccg aacaattgaa ccacttcatc gcctgtatca
     3721 ggattccaca gccgcgccgt attatcccgg cttgcagtag caacatagtc gtcgttcggg
     3781 ctgaacgcgg catccgttac ttcggcggta tgtcccttca gggtctgcaa tacttctcca
     3841 gtagccgtgt cccatattcg ggctgtgcca tccacactgg atgtgacgat ccgctttcca
     3901 tcagaactaa aagcggctcg cgtgaccaat ccggagtggc cggcaaatat tcttagcggt
     3961 tcacctgttt cggcattcca aagccgggcg gtaccatcgg cactggcagt gaccgcgagc
     4021 ttcccatcgt cgccgaacat tgcgctggca acccagtccg tgtgcccttc gaggatcacc
     4081 tgcaattctt tggtgtctgc gtccaggatg taaccaaagg tgtcgcggtt ggcgatgatg
     4141 attcgcgttc catctttgtt gaagcgagcg tcgtgaacga tgcttgtaat aaaatccgtg
     4201 ctgctcaatt gttgggctgt atccacatcc caaattcgcc atgttccatc gccactggct
     4261 gtgatcaatt gctgatcatc cgggctgaag gcaactccgc tggccaacca gccgggatac
     4321 tcccaatgaa tttgctcgtg acctgtctgt gcattccaga tctgaatggt tcgatcttcg
     4381 ctagcggtgg caatgagttg tccgttggaa ctataagcag cgcctgtaag tttgtccgtg
     4441 tgccctatca ggatgcgctt gcgccattcc ccaatattcc aaattctcac ggtttgatct
     4501 tcgctggcgg ttgccagatg attgcccgtt gggtcgaaca caacttcgtt aacagttccg
     4561 ctgtgccctc gcaacacttc gatgctgcgt gcagtttctg tatcccagat tcgtatcgtg
     4621 tgatcgcgac tcgctgttac cacttgtctg ccatcccctg ggcttatagc tccactgatc
     4681 acgtaaaagg tatgcccttc aagatattcg gttagcacaa acgcgttttg aatatcgagc
     4741 aagcgcggga tgtagcctcc cgtggttaac acatattgtg aatcttcgct aaacgcaagg
     4801 tcagcaactg cctcgttgta tggtgcatcg ccgtttgaaa tctcattgat aaactcgcct
     4861 gttgctgcgt tccaaatctt tacaagagta tcattgccgc ctgtagcgat caattttcca
     4921 tctgggctga aagctgcgcc aagcaccgca gcggagtgtc cacgcaaaac atacatctga
     4981 cgaccagtgt cgacgttcca aacacgtgcc gtggcatcat cactggccgt gaccagcatt
     5041 tgtccatccg gactgaatgc aacactgttt acggagcttt ggtgtttcat gctgcgcagt
     5101 tgctcaccgg tcacggtgtc ccagatcatt acaattccat ctgcgccagc cgtggcaaca
     5161 agccgaccat ccggactaaa agccgcgtct ttcaccgctc cttcatgact acttaatgca
     5221 attaactcct cacctgtcgc agcatcccag atgcgggcgg tcccatcctc gctggccgtg
     5281 acaaggcgtt tgccatcgga atcataagca acgctgtaca ctgtgccgga gtgaccgcgt
     5341 agggttcgct ccactggtgg cgataatagt gcacgtctca gtgcttcctc gccttgcaag
     5401 gtttgcttct tgtcaatgga caggattgct aaatgcaagg agagttcggg atccaagtca
     5461 agtttgttga tcgagctggc ggcgagttcg cgtgccaggg caaggtctgc ttgtctttgt
     5521 gcctcttgtt cgttcgcctg tgccgtgcca gcgttggcaa ctgctaatat actcgcagct
     5581 tcggccacct ctgcattggt tgttgcccgc cccgcctgaa cccatgcaac ggcagacaga
     5641 acgagcaatg caatgatcac tccaattcca atgctgcgtg tgcgacgcct gctaacatca
     5701 ctagcctgcc ggctcttgaa aacatattca cgctgtaaat cggtgggatg aggttctttg
     5761 gatgtgtttg tggccagctg aaattcggca tcctgtagtt ccttgccgtg taaaagaaaa
     5821 ctatcctctt tgccacttcg gtcccattcc agggctttga cttgcaactg gcgatggacc
     5881 tgtgcccagg caaagtctgt tttgatagcg atcagtaatt tgttaaaagc aggctcaaat
     5941 tcatcggttt ccctgaagaa catatagttc aagtggctga gcgcttcagg tgattcatca
     6001 ctctggattt cacgtacaac cacaggaatc aaccgtttgc cattcttaat ggcgtggtca
     6061 atctcctgcc tgcaaatttt cgatttggtt gaatccgggc tgatcaggaa gagaaagata
     6121 tcagcttcct cgattccctt ttcgatctcc ttccaccaat ccacggtagg tgggatgcct
     6181 tcccagtcaa tccaaaagtc cagttcctga cccttgaagg catcggtaag ccggcgtgcg
     6241 gcgtcaatat ctctgcgcga ataggagatg aaaagactgg tcattttata gccccataat
     6301 ccttccccca aatccgtttt tgatttgggg gaaggcaggg aagggggcaa aaacacctgc
     6361 gacctgcgca ttcaagtcac ttgccaggcg ggtaacgaat tcctggtccc ggcgtgaata
     6421 ggagatgaag atatcttttg tcatgggaag gattcaagcg aagcttcgta cgtagccatt
     6481 ggcacaactc cgccttttca aataactcaa tcattctcgg cttgccagac cttgaatgaa
     6541 cacgctgtag cttagcgcga gccttcagaa gttgtcccgc tgcggaagcc caccttacga
     6601 tgactcccgt cacagaacgg cttgttctca gagtgaccac agcggcaaag ggctacgcgc
     6661 gtatcctcgc gaatcagaga accacctgat cccataattc gcatatggcc tcggacatat
     6721 aaaggtccgt tgagtcgcgg gttgatcgtc gtactct
//
LOCUS       DASTJP010000112         8982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_494872, whole genome shotgun sequence.
ACCESSION   DASTJP010000112 DASTJP010000000
VERSION     DASTJP010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8982
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494872"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1553
                     /locus_tag="VFQ23_03495"
     CDS             321..1553
                     /locus_tag="VFQ23_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9905670.1"
                     /translation="MKEHLSILKQGVGAWNVWRAKNIYTYADFNETDLNGADLRGIDL
                     RLAHFRRANLFRSDLKGANLSGADLSDADFSQANLSEANLNKANLKGASLSGAFVTEA
                     NLSQANLLAANLRNANFNDVNLSDADLTNARLSGTSLRRADLSGARLLRSNFTKADLV
                     EAKFIHVDLNETDISEAKVGGTVFADSDLSKLIGLARVDHCGPSHISVDTFIRSRGRI
                     PEIFLRGCGLSDWEIEQSKLYNPDLNDDEINKILHRIHDLRAAQALQISPLFVSYSQA
                     DDMFVQKMGNCLKTRGIRYWRDVHVVKSGRLEQQIDKAIRQKPTVLLILSEHSLSSDW
                     IEREVRTSRELEGEMDRRVLCPVALDDSWKSSRWSKRLMEQLTQYDILDFSGWEEDAR
                     FDGMFSKLIDGLELFYKG"
     gene            complement(1955..2878)
                     /locus_tag="VFQ23_03500"
     CDS             complement(1955..2878)
                     /locus_tag="VFQ23_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9905671.1"
                     /translation="MLLERVFSRNNLERVGKVLVQTFKHFTKDNAPQWAAAIAYYSLL
                     SIFPLLLAGVAIAAYFVDQQWAIEQSSRLIRGLVPSGTQFLRETIESIIESRGQVGFL
                     SIMILIWSGSRVFGVITRALNIAYHVSEPYSFFRRTLIELLMTFTIGVLFVLSLGWRL
                     LITFIEGIVQISFFEGQVFYQFLSNAVSAALLLLSLFLIYLYVPRRRVAWWAALAGAT
                     LFTVLFLLAQPLFTGYIQTFANYSLVYGPLAIVITLLLWIWISANLLLLGGELASHIQ
                     DRLVEEKSEKEVEEQHEERDPTSPTHEQPLH"
     gene            complement(3210..4382)
                     /locus_tag="VFQ23_03505"
     CDS             complement(3210..4382)
                     /locus_tag="VFQ23_03505"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905672.1"
                     /translation="MNRLTKSDLRALLEYSGFPHLTFYMPTHRAGHEVQQNPVRFKNL
                     LREAENALSGHDLTVPDIQEFLEPARSQLAMNETFWQQQSDGLVIFIAPELLQYYRLP
                     IDFEEAVLVGNQFYITPVLEVFQNAGQFYILALSQNETRLLEGTKDEVKEVEIKQLPP
                     SLVEALKWDDPEKQLQGHAVSRGRQGGERGRPDMSFHGHGSGKDDDKTNILRYFRMVD
                     KALSDFFANERIPLVLAGVEYLLPIYREANSYNYLAEQGIPGNPDALSAEVLHTRAWE
                     LLAPRFKMEQVEAAALYRQQAGQQTGRTLNDVVAIVQSAYAGRVGQLFIQKGSEIWGR
                     YDLANNQVELHPERIAGSEDLLNTAAVYSFQNDGVTHVVEQNDMPDEASIAAILRY"
     gene            complement(5173..8547)
                     /locus_tag="VFQ23_03510"
     CDS             complement(5173..8547)
                     /locus_tag="VFQ23_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET9905673.1"
                     /translation="MKKKSSGLSMMVILSLLINGLVSPFASASPALAATLPVIDDFES
                     GLPSGTDGDGAAIGFITFRDPNSSVAISTTDAPPAPVPGASSPNNVLKMDLSVVSFAG
                     FVHNFENAAVDTWVPQDWSAYEGISFWLYGNNSGTTMFVDVLDNRNPGSTRDDAERWS
                     IDVTDNFSGWREIQIPFESMHRKEIGNGAPNDGFGLTEVHGWALGTIITPAPQTYYVD
                     NAMVYGVAPVRPLTVGFTAIDYRFTEGTTATLTARLSKPSADPVSVQYATGFGLAVPN
                     RDYIPTSGTLTFPPNITQQSFTIQTIDNQKYQGERGVLVQLSNPTGGAQLGIPPITRL
                     NILDNESFDPTLLDDFETYPYLWQVDRKAQLSNPEIAAGDPLALPGQGAYEHILQAGQ
                     RNGRGTYSFSRTFPIGQDWSDLGGLNFWYYGRNSNKDIGVSLDNNLSAVGNPSDWRLV
                     WSDEFNARTRTAPNASVWGQEVGDGTVNGIPGWGNSELEYYTDGTNNVSTDGQGNLQI
                     TVKEADGSLTCYYGPCEYTSARLLTKNRFEVAYGRLEARVKVPEGAGLWPAFWMLGTD
                     IDQVSWPQTGEIDIMEFVGRVPNEVFGTLHGPGYSGGQSYGDTYDVGEPVGDQYHVFS
                     VEWQPDYIAWYIDGIQFFAATPDDPFLQGKQWVFNHPFFILMNVAVGGNFGGPVGEDT
                     VFPQSMLVDYVRLYQTRPTPVSFNATFRDDFTGWRQISLPFSAFQNADGHVLDLSNIQ
                     TLRFEIPDGLRNPVMLDQIRLSAPCDVTVTNTADAGVGSLRRALASVCAGGTVTFSEA
                     LAGQTITLLTGPLTLGKNVTIDASAAPGLTISGNDTDRVFIVNAGTTATVKHLTVTNG
                     FGFQLAGGILNNGSLTLDHVTVTDNTMTTNAGDFWQGGGGIYSGDGATLNLIDSSVSN
                     NQAAWSGGGVYAFFNTTTTILRSTISGNVSNDVGGGIRSLGDMTITNSTISGNRSTGW
                     HGGAIFQTDGDITITNSTIANNIGPDWAPSTFFIGQFGGSFVPTLTLTNTIITGNQWY
                     ACERFASGTAANVLSGGNNLVQDDSCNPVASDLINSAALIGPLANNGGPTQTHALLSG
                     SPAINAANDALCPPTDQRGITRPQGMRCDIGSYESP"
BASE COUNT         2268 a         2190 c         2256 g         2268 t
ORIGIN      
        1 tcaatgcttg aatcacattc cgccgttcct cttggaggtc agcgtatgta gaactgacga
       61 atacctgata acgtttttcc atccttcatc ctttcatcaa tgccctatag caggattcta
      121 cctgaaatgg gatactgcat ctgaccttgg ggtagtcaaa ttggtggcaa aagtattgcg
      181 atttccacgc tcagagggtg aagtctccgg caccagaaat gtaataactc tgcatttttc
      241 agggagagga tttgccttat acttttgtgg aaagcaggta aattcaggtc cgctacggga
      301 aagagaaata gtcgatgcag atgaaagagc acttatccat tttgaaacag ggtgttggcg
      361 cgtggaatgt atggcgagcg aaaaacatct acacgtacgc agacttcaat gaaactgatc
      421 tgaatggagc cgatctgagg ggaatcgacc ttcgattagc tcattttcga agggccaatc
      481 tgtttcgatc tgatctaaag ggagcaaatc tcagtggcgc cgacctcagc gatgccgatt
      541 tcagccaggc caacctcagt gaggcgaatc taaacaaagc gaatctcaaa ggagctagtc
      601 ttagtggtgc ttttgtcaca gaagccaatc tcagccaggc gaatctcctg gctgctaatc
      661 ttagaaatgc caatttcaat gatgtcaacc tgagtgacgc cgatctcacg aacgctcgtc
      721 tttccgggac cagtctgcgt cgagctgatc taagtggagc ccggctcctc agaagcaatt
      781 ttacaaaagc cgatctggtt gaagccaagt tcattcacgt ggatctgaat gagacggata
      841 tttcagaggc gaaagtgggt ggcacagtgt ttgcggattc tgatctcagt aagctgatcg
      901 gtttagcaag agtagatcac tgtggtcctt ctcatatttc agttgataca tttattcgtt
      961 cgcgagggag gattccagaa atcttcctgc gcggttgtgg gttgagtgat tgggagattg
     1021 aacagagtaa gttatacaac cccgatttga acgatgatga gataaataag atcctgcatc
     1081 ggatccatga tttgcgtgcc gcgcaagcgt tgcagatctc gcctctgttt gtctcttaca
     1141 gccaggcaga tgatatgttc gtccagaaga tgggcaattg cttgaagact cggggtattc
     1201 gctattggcg cgatgttcat gtagtgaaat ctggaaggct ggagcagcag atcgacaaag
     1261 ccattcgtca aaaacccacg gtgcttttga tactttctga acattcgttg agcagcgatt
     1321 ggattgaacg tgaagtccgc acctcgcgcg agttggaggg tgagatggac cgccgtgtat
     1381 tatgtcccgt tgccttggat gacagctgga agtccagtcg atggtcgaaa cgtttgatgg
     1441 agcagctcac acagtatgac atccttgatt tttctggatg ggaggaggat gccaggtttg
     1501 atgggatgtt cagcaagttg attgatgggt tggaattgtt ttataaagga taaagtaaag
     1561 tatagatacc acctggtttt tacacgtaaa gattaggata ctctggcgct cttggtcgtg
     1621 agtctctcac tacgggcact tcgcaaatct ccccctcggc acctatgacc cacccaatct
     1681 ggcgggtgtt gtgcttggat ggcgattaac ttaagaattg aagattgtca tttgatatgt
     1741 ttgtctggat ggctgactct ggttgcccac gatcaccgag tacgcatccg cctctgaagt
     1801 agaagacgcc ccacaggttt ccaggtataa aagacgggat cctaaggaca gtatcaggat
     1861 ggacgcgggc aataggatat gaggattacg atgcgtctga acgccgtaca cgatgaagac
     1921 gagaaaagga cagatcttcc attttgccag ttcatcagtg caaaggttgc tcatgcgtcg
     1981 ggctggtggg atcgcgttct tcgtgctgct cttccacttc tttttcggat ttttcttcga
     2041 ccagcctatc ctggatatgt gaggcaagct cgcctccaag aagcaggaga tttgccgaga
     2101 tccagatcca gagaagcagt gtaatgacaa ttgcaagcgg accatacacc aggctgtaat
     2161 tggcaaaggt ctgaatatag cctgtgaaga gaggttgggc gagcagaaaa agtactgtga
     2221 acagggtcgc gccggcaagc gccgcccacc aggccacccg gcggcggggc acatatagat
     2281 atattaggaa cagactaagc aaaagcaatg ccgcggagac ggcattgctc aaaaattggt
     2341 aaaaaacctg cccttcgaaa aatgaaattt gcacaatgcc ttcgataaat gtaattagaa
     2401 gccgccagcc gagagatagc acaaatagaa ccccaatcgt gaaggtcatc agcagttcga
     2461 tcagtgtgcg cctaaaaaaa ctatagggct cactgacatg ataggcgata ttcagggccc
     2521 ttgtgatgac gccaaagacg cgcgaaccgc tccagatgag gatcatgatc gaaaggaagc
     2581 ccacctgccc gcgtgactcg ataatgcttt cgatagtttc acgaaggaat tgcgttccgc
     2641 tcggtaccag tcctctgatc aaccgggaac tttgctcgat agcccactgt tggtctacaa
     2701 aatatgctgc gatggcaacg ccagccagca gcaatggaaa gatggacaac aaactataat
     2761 aggcaatggc agctgcccat tgtggagcgt tgtcctttgt gaaatgtttg aaggtttgga
     2821 cgagaacttt cccaacacgc tccagattgt ttcgcgaaaa aaccctttct aatagcatat
     2881 ttttgtgatc ccttctatga tgactccaca ccggtctgtc cgcggtttgg tgggacgaac
     2941 tttttgcgta cccacgtgcg caggacaaaa gcaagcggcc atccaatcag gtctccccgg
     3001 agtccaccgt tcaccgatcg ctccgcctgc aaaacgtggc ggaatggttc gcagactcag
     3061 gctgtcagtt ccgcatccac gtaagtgtat gagtcacccg taacaggaat ccagcatcca
     3121 aataaacaac acgagcttaa gaagcaaatt tttacactgt cttagttcac agttttcttc
     3181 cctgattcaa aaagaccttt aaagcgtgtt caatagcgaa ggatggcagc aattgaggct
     3241 tcatcgggca tgtcattttg ctcaacaaca tgagttacac catcgttttg gaaggaatag
     3301 acagcggcag tattcaataa gtcttcgcta ccggctatcc tttctgggtg aagctcaacc
     3361 tggttattag caaggtcgta tcgtccccaa atctcagatc ccttttgaat gaatagttgt
     3421 cctacccgtc ctgcataagc ggattgcaca atggctacaa cgtcgttcaa cgtgcggccc
     3481 gtttgctgac ctgcttgttg ccggtaaagt gcggccgctt ctacctgttc cattttgaat
     3541 cgaggtgcca acagctccca ggcgcgagta tgaagaactt cagcgctcag tgcatcagga
     3601 ttgcctggaa tgccctgctc tgccagataa ttataggaat ttgcttcccg atagatgggg
     3661 agcagatatt ccacgccggc aagcaccaat ggtatccgtt cattcgcgaa aaaatcggat
     3721 agagctttgt ctaccattcg gaagtagcga agaatatttg tcttgtcgtc atctttgcca
     3781 ctcccatggc catggaaact catgtctggt ctgcctctct cccctccttg acggccccga
     3841 cttactgcat gaccctgaag ttgtttttca ggatcatccc acttcagcgc ttccaccaga
     3901 cttggaggca gctgtttgat ctctacttct ttcacttcat cttttgtacc ctccaaaagc
     3961 cgggtctcat tttgactcag tgccagaata tagaactggc cggcgttctg aaaaacttca
     4021 agaacgggtg tgatatagaa ttgatttccc acaagtacag cttcttcgaa atcaataggc
     4081 aaacggtaat actgcaggag ttccggggca atgaagatga ctaaaccatc actctgctgt
     4141 tgccagaaag tttcattcat cgccagttga cttcgtgctg gctcgagaaa ttcctgtatg
     4201 tctgggacgg tcaaatcgtg gccggataaa gcattctcag cctctctcaa caggttctta
     4261 aaacgaacag gattctgctg cacttcgtgc cccgcgcgat gagtcggcat ataaaaagtc
     4321 agatgcggaa aacctgaata ttccaataaa gctctaagat ctgattttgt taatcggttc
     4381 atacgaatct ccagtcgcaa atgtagaaaa gagtaccagt ttattcacag gaaataattg
     4441 gctagtcttg ccggccaact atctgccgct acccccgggt ccgcaagtgc gtgataaacc
     4501 agaatgttgt aaaattatcg cctgctgatc aaaatcatcg ctgcggaacg tttagtgcgg
     4561 ttttaagctc cccagagtag cactacaaat attgtagacg gttaaggtaa gtgttcttca
     4621 attaatcaca tcaccctagc ctttcttacc gtgttcttga ctcattttta cccccaaaga
     4681 attcgagtcg taagtaggga aattcccaat tgtcatataa tcttgatgag tttacaatgt
     4741 gaaagagcac tgacggttat ttaagaggga gaatttcaac tctattttct aaatgaaaaa
     4801 tgagattcct ttaaggcctg tagagaatgt ttcatatgaa caacaaatcc atgcgtccct
     4861 ggtcgcgagc tctcttgccg cgtaaatttc cccttcggca ctaaaagtgg gccgtcaggg
     4921 ccctcagtat ctggtggttt cttgctaaaa agaccaccat tttctttttt ttctccaaaa
     4981 cacaagaata tcgccgaaat ccaaggtcat aaaatcatgg aattttcttt attaataaga
     5041 cactaccctc atatataaac gaaaataaaa tacgtgctac cccggtatcc aatcatgaaa
     5101 cagggccagg aaaacagtgc taaacaaaaa aaagaccacc tcatcaggtg gtcttttttt
     5161 tcaaatctgg acttaaggag attcgtagga tccgatgtca catcgcatgc cttgcggacg
     5221 cgtgatgccg cgctggtctg tcgggggaca gagcgcatca ttcgcggcat taattgccgg
     5281 gctgcctgaa agcaacgcgt gggtctgggt aggcccgccg ttatttgcta atggaccaat
     5341 cagagctgca ctgttgatca ggtcacttgc caccgggtta cagctatcgt cctgtaccag
     5401 attattccca ccggaaagga catttgccgc ggtgccggat gcaaaacgct cacacgcgta
     5461 ccactgattg cctgtgatga tcgtgttggt cagtgtcagg gtgggaacga agctgccgcc
     5521 aaactggccg atgaagaatg tggagggagc ccagtctggt ccgatattgt tggcgatggt
     5581 tgagttcgtg atcgtgatat ccccatccgt ctggaagatc gcaccgccat gccagcccgt
     5641 tgaccggttg ccactgatcg tgctgttggt gatcgtcata tctccgaggg agcggatgcc
     5701 accgccgaca tcgttggata cattgccgct gatcgtgctg cggaggatgg tcgtcgtagt
     5761 gttgaagaac gcatagacac caccacccga ccaggcggcc tggttgttgc tcacactgct
     5821 gtcgatgagg ttgagagtcg ctccatcacc gctatagatg ccaccaccgc cctgccagaa
     5881 atctccggca tttgttgtca tggtgttatc agttacggtc acatggtcca gcgtcagcga
     5941 gccattgttg aggatgccgc ccgcaagctg gaagccaaag ccgtttgtga cggtgagatg
     6001 tttaacagtg gcggtcgtgc ctgcattgac gatgaacacg cgatcggtgt catttccact
     6061 gatcgtcaag ccgggtgcgg ctgaagcatc aatggtcacg tttttaccca gagtcaatgg
     6121 gccggtaagt aacgtgatcg tctgaccggc gagagcctct gagaaggtca cggtgccgcc
     6181 cgcacatacg ctggccagtg cccgacgcag tgagcccaca cccgcatcgg ctgtgttggt
     6241 gaccgtgacg tcgcacgggg cactcaatcg aatctggtcg agcattaccg gattgcgtaa
     6301 gccgtcgggt atttcaaatc gaagtgtttg gatgttcgaa aggtcaagga catgtccatc
     6361 cgcattctgg aaggcggaga acggaaggct gatctgacgc cagcctgtga agtcatcgcg
     6421 gaatgttgcg ttaaaagata caggtgttgg tctcgtctga taaagacgta catagtcaac
     6481 cagcatgctc tgagggaaga ctgtgtcttc tcccaccggt ccgccaaagt tgccgccaac
     6541 tgccacgttc atcaggataa agaagggatg attaaagacc cactgtttgc cctgcaaaaa
     6601 cggatcgtct ggagtcgcgg caaaaaattg aatgccgtcg atgtaccagg cgatatagtc
     6661 tggttgccac tcgactgaaa aaacgtgata ctgatctccg acgggttcac caacatcata
     6721 ggtatcgcca tagctttgtc caccggaata tcctggaccg tgcagcgtcc caaacacctc
     6781 attcggtacg cgccctacaa actccataat gtcgatctca ccggtctggg gccaacttac
     6841 ctgatcgata tcggtgccaa gcatccaaaa tgcgggccat agaccagcac cctcgggtac
     6901 cttgacgcgc gcttcgaggc gaccatatgc cacttcgaag cggttcttgg tgagcagccg
     6961 cgccgatgtg tactcacatg gaccgtaata acaggtcaat gatccgtcgg cttccttgac
     7021 tgtgatttgc aaattgccct gaccatcggt cgacacattg ttcgtgccgt cagtgtagta
     7081 ttccagctcg ctgtttcccc atccaggaat gccgttcact gtgccatcgc ctacttcctg
     7141 accccatacg ctggcattgg gtgcggtcct ggttctagcg ttaaactcat cgctccacac
     7201 cagcctccag tccgaaggat tgccgacggc tgagagatta ttgtccaggc tgaccccaat
     7261 atccttgttg ctgtttcgtc cgtaatacca gaaattcaat ccgccgagat cgctccaatc
     7321 ctgtccgatg gggaaggtgc ggctgaagga ataggtgcct ctgccatttc tctggccggc
     7381 ctgcaggatg tgttcgtaag cgccttggcc cggcaatgcc agcgggtcgc cagctgcaat
     7441 ctccggattc gaaagctgcg ctttcctatc aacctgccaa aggtaaggat aggtttcgaa
     7501 atcatccaac aaggttgggt caaacgattc attatcaaga atgttcaatc gcgtgatcgg
     7561 aggaataccc agctgtgcgc cacctgtagg attggaaagt tgcaccagca caccacgctc
     7621 gccctgatac ttttggttat ctatggtttg gatcgtaaag gattgttgag tgatatttgg
     7681 tggaaaagta agcgttccac tggttggaat gtagtcgcgg ttcgggactg ccagaccgaa
     7741 gccggtggcg tattgcaccg ataccggatc agcagagggt ttgctgagcc tggctgtgag
     7801 cgtggcagtc gtaccctcgg tgaatcgata atcgatcgcc gtaaagccga cggtcagagg
     7861 gcgaaccggt gctacgccat acaccatcgc attatccaca taatacgtct gtggagcggg
     7921 agtgataatt gtgcccaacg cccagccatg cacctctgtc agaccgaacc catcgttggg
     7981 cgctccgttg ccaatctctt ttcgatgcat actttcaaag gggatttgaa tttctcgcca
     8041 gccgctgaag ttatctgtaa catcgattga ccagcgctct gcatcatccc tggttgaccc
     8101 cgggttgcga ttatcgagca catcaacaaa catggtagtg ccactgttat tgccgtatag
     8161 ccagaaggaa attccttcgt aagcactcca atcctgcggt acccaggtgt cgacggcagc
     8221 attttcaaag ttatgcacga agcctgcaaa cgaaacaaca cttagatcca tcttcagcac
     8281 attgttcgga ctggacgcgc cgggaacggg cgcaggaggg gcatcagtgg tggagatcgc
     8341 cacactgctg ttgggatccc tgaaggtgat aaacccgatt gcagcgccgt caccatctgt
     8401 cccgcttggc aatccggatt caaaatcatc aatgacagga agcgtggctg ccagcgcggg
     8461 actggcagag gcaaacggag aaaccaatcc gttgatcaac aaactcaaaa tgaccatcat
     8521 cgacaaacct gaactcttct ttttcatcct ttattctcct cgtaaaattg gatttcgtga
     8581 gatggggatt tgtttcttga tgcaacttga tgagctacta aattatggaa attttttctg
     8641 gtgttataca gattttactc gttctggact cggtgaatat gagaaaatgt aacactgcct
     8701 attcgatggc agcagaagaa ttcacgattt tagagtttta ttattatata tgttacgcga
     8761 tatttgtaac gtaagaaata gtggggagtg gcgaatgtag tgtggaggca acagagccga
     8821 cagagttttt gagttttttc cacctgcccc gaacgcaggt gcggggtcaa agggctcagt
     8881 gctctcggtg gctaagtaga ttttcaatcg ctctcttcgc cactcccaaa tagtgactca
     8941 tgagcccctg gtcatgagta tccgttacga ggacttcgca aa
//
LOCUS       DASTJP010000113         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1192284, whole genome shotgun sequence.
ACCESSION   DASTJP010000113 DASTJP010000000
VERSION     DASTJP010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192284"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFQ23_03515"
     CDS             <1..381
                     /locus_tag="VFQ23_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET9905674.1"
                     /translation="FGKFISNLAFMLLVEIIVLPIYSILYNTNLFQLGLLMVILLGSI
                     GYTAVGTLLAAMSVQTRTRDVLLPILLFPVAFSVLLAAVKASGGFLDNSELADILLPI
                     NLLIAYDIIFISVSFMVFDSIVEE"
     gene            398..1096
                     /gene="ccsA"
                     /locus_tag="VFQ23_03520"
     CDS             398..1096
                     /gene="ccsA"
                     /locus_tag="VFQ23_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9905675.1"
                     /translation="MQPKPLLLKILDVISILVLAVAAYLAIVVAPTERVMGDVQRVFY
                     FHVGTAWTALLGFIFSAVLSVIYLVTKDLKWDRIQVAAIEVSLVFFLITIVLGSIWAR
                     PAWNTWWTWDPRLTTATITELIYIAYFMLRQGIDDPDRRARFGAVYALLGGLSAPITF
                     FAIRLFRTIHPVVIGGSNPEADGSFSMTGDMLSVFFFALFAFTVIFIDLFWNRIRLGN
                     LQDKVEQLKIKVTI"
     gene            1158..1391
                     /locus_tag="VFQ23_03525"
     CDS             1158..1391
                     /locus_tag="VFQ23_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905676.1"
                     /translation="MFLQEPTPDTSGYMIAGYAIAFIVMGLYVFSLYLRNRNLKQDLT
                     MLEELDKAELEADASPPEKPVKTVTKKGRKKQP"
     gene            1475..>2554
                     /locus_tag="VFQ23_03530"
     CDS             1475..>2554
                     /locus_tag="VFQ23_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905677.1"
                     /translation="MLVSILAGIVSVASANGLAAPASAPPGPDRFSVVVVDYTKYTWW
                     LIRWGEKEVECKIKVDHEGLPTPGDIYIDCGEAVYDKWITQKPCLATNTDECKGYYLI
                     LVDSEPAQKEIATKLPPPTVHITLENCNPVYTSSTSICEFEPILVLTGLEPLPDYNII
                     AIEGLYGDQPFTCGPICRLQLPITGEQGVNLQFWAYSSYGDSSEIFNALVRVALADEG
                     DPDRTFWYVDVLSSQWIGVPVASCVQAWGVLPPVGGPPEWLSTPTESENLGTDVPYNY
                     LAEQLIRSGMVDVSACADGGLLPDGGTSACGMDAARGAVTQWQNQFDEVILNVAKDSG
                     VPAHLLKNIFAVESQFWPGAVFKTDI"
BASE COUNT          598 a          611 c          650 g          695 t
ORIGIN      
        1 ttcggcaaat ttatcagcaa cctggccttc atgctgctgg tagagatcat cgtcctgccg
       61 atatatagta ttctctacaa tacgaattta ttccagctcg ggttattgat ggtgatcctg
      121 cttggttcca tcggatatac agccgtgggt accttgctgg ccgccatgtc tgtccaaacg
      181 cgtacacgag acgttttgct gcccattctg ctttttccag ttgcattttc ggttctattg
      241 gcggcagtca aggcgagtgg aggctttctc gataactcag aattggcgga tattttgctg
      301 ccgatcaatc tgctgattgc ctatgatatt atttttattt ccgtgtcatt tatggtattc
      361 gatagtatcg tagaggaata agattacagg agtcaaaatg caaccgaagc ctctcttgtt
      421 aaaaattctg gatgtaattt caattcttgt tttggctgtt gctgcctatc ttgccattgt
      481 cgtggcaccc acggaacgag tgatgggtga cgtgcagcgc gtgttttact tccatgttgg
      541 cacagcctgg acagcgttgt tagggttcat tttctctgct gtcctgagtg tgatctatct
      601 cgttacaaag gacctcaaat gggaccgtat tcaagtggcg gcgattgaag tcagcctggt
      661 atttttcttg atcaccattg tgctcgggtc catctgggca cgccccgcct ggaatacctg
      721 gtggacctgg gatccccgcc tgacgaccgc gaccatcact gagctgatct acattgcgta
      781 ttttatgctc cgccagggaa tcgacgatcc ggatcgccgt gcccgcttcg gagccgtcta
      841 cgccctgctg ggcgggctga gtgcgccaat taccttcttt gcgattcgat tgttccgcac
      901 gattcacccg gtcgttatcg gtggctcgaa ccccgaagcc gatggcagtt ttagcatgac
      961 cggtgacatg ctgagtgttt tcttctttgc gctgtttgct ttcacagtca tcttcattga
     1021 tctattctgg aatcggatcc gccttggcaa tttgcaggac aaggtcgagc aattgaagat
     1081 aaaagtaaca atctaacaat ctagtcgtct actctattcg ggatcgagat ccgaatgagt
     1141 aaaacagtgg agtctacatg tttcttcaag aacctacccc ggatacgtcg ggatacatga
     1201 tcgccggtta cgcaattgca tttattgtta tggggctata tgtatttagt ctctatttgc
     1261 ggaatcgcaa cctgaaacag gatttgacaa tgctcgaaga gttggacaaa gctgaattgg
     1321 aggctgacgc cagcccacca gagaaacccg tgaaaactgt aaccaaaaag ggacggaaaa
     1381 aacagccgta acccgcggga gtccgctata atgtgaccgg actaattttc ttatgcgatt
     1441 ttgggttgga tataggtctc tgctgcggat aataatgctg gtctcgattc tggcgggaat
     1501 cgtatccgtg gcttctgcaa acgggcttgc cgcgccagct tccgccccac ccggaccaga
     1561 tcgcttctct gtcgtcgtag tggactacac caaatataca tggtggctga ttcgctgggg
     1621 tgaaaaagaa gtagagtgta agatcaaggt cgaccatgaa ggtttgccaa cacccgggga
     1681 catctacatc gattgcggtg aggcagttta tgacaaatgg attacgcaaa aaccctgttt
     1741 ggcaaccaac accgacgagt gcaaaggcta ttatttaatt ttggtcgata gtgagccagc
     1801 ccagaaagag atagcgacca aacttcctcc acccaccgta cacataaccc tcgaaaattg
     1861 caatcctgtt tacacctcat caacaagtat ttgtgagttc gagcctattt tagttttaac
     1921 tggtttggag ccacttcccg actataacat catcgccatt gaaggattat acggggacca
     1981 accttttacc tgtgggccca tatgcagatt acaacttccc atcacaggtg aacagggagt
     2041 caatcttcaa ttctgggcct attcaagtta cggcgatagc agtgaaattt tcaatgcact
     2101 ggtgcgggtt gcactggctg acgaaggcga cccggatcgc acgttctggt atgtagatgt
     2161 attgagcagt caatggatcg gtgtgcctgt tgcgagctgt gtacaagcgt ggggtgtatt
     2221 accgcccgta ggcgggccgc cagagtggct cagtacaccc actgagagtg aaaatcttgg
     2281 caccgatgtt ccctataatt acctggctga acaattgatt cgcagtggaa tggtggatgt
     2341 aagcgcctgt gcagacggcg gactcttgcc ggatggcgga acgtccgcct gcggcatgga
     2401 tgctgcccgc ggcgcggtca cgcaatggca aaatcaattt gatgaagtca tcttgaatgt
     2461 agcaaaggac tccggtgtgc cggcacacct gctcaaaaat atcttcgctg tggagagcca
     2521 gttttggccc ggagccgttt tcaaaacgga catc
//
LOCUS       DASTJP010000114         8501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1003345, whole genome shotgun sequence.
ACCESSION   DASTJP010000114 DASTJP010000000
VERSION     DASTJP010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003345"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFQ23_03535"
     CDS             complement(<1..338)
                     /locus_tag="VFQ23_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905678.1"
                     /translation="MPISPRVLLSIDYEPVFALFRHYDQIADVKTRRDLDARFTLEAV
                     DPILEKLKDSKVSIYLVGEIAEWYPEVPQKIVAAGHELGLHCQFHRPLVDVNELADDV
                     RASASWCRQY"
     gene            complement(424..1374)
                     /gene="arcC"
                     /locus_tag="VFQ23_03540"
     CDS             complement(424..1374)
                     /gene="arcC"
                     /locus_tag="VFQ23_03540"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET9905679.1"
                     /translation="MTKLAVVAIGGNSLIKDNKKVTVESQMQALQETARHLVNMIDAG
                     WDLAIGHGNGPQVGFILRRSEIAAKAEGMHEVPLDVCGADSQGAIGYELQQALQNEFH
                     RRGIKKKACTIVTQVRVDQNDPAFQKPSKPIGSFMDETEAKRREQEMGWSVAEDAGRG
                     WRRVVPSPLPKEIVEFEAVKLLLDAGHIVITVGGGGIPVIDAGNGELEGTAAVIDKDF
                     ASSLLARSLKADMFLIATAVDKVAINFGKPDQKWLDNINVAEAKAYLEEGIHFAKGSM
                     APKIQAAIWYLENGGKQVLITCPENIGRALKGETGTWISS"
     gene            complement(1474..2454)
                     /locus_tag="VFQ23_03545"
     CDS             complement(1474..2454)
                     /locus_tag="VFQ23_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET9905680.1"
                     /translation="MQTNFRGRDFIGDLDFSKEEVETVLDVAWDLKRKRALSEPHALL
                     RDKVLAMLFFFTSTRTRGSFEAGMAQLGGHAAFIDSETTQISHGDTAKEIGEIFGRYF
                     DGIAIRQCDWQFGNQYINDVAKASRVPVLNMQCDVYHPFQCLADIMTVIEKKGRDLKR
                     KKVVVSWAYAASYSKPISVPQSLILQMTRFGCDVVLAHPPEFKLMPDIMQQAQDNAKK
                     YGVGFDVTDNMDEAFKDADVVYPKSWGPLLTTTGKEEGKALIDKYKSWITDERRMDLT
                     KEDSIYMHCLPADRGLEVTDGVIDGRHSVVYDEAENRLHAQKAVMALTMS"
     gene            complement(2600..3124)
                     /locus_tag="VFQ23_03550"
     CDS             complement(2600..3124)
                     /locus_tag="VFQ23_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905681.1"
                     /translation="MKAIEIRRHSIRHQPGDHLNQTGVTLARRVGQNIGPFDLVVTST
                     LPRAFETAIAMGFAVDEQIELMSTYGRDVGREAPWPLSLAGYAEVVSQGGAAARYANQ
                     LADVYRKLLHYVSDDHAALVINHGGILEMGAVACLPDANHLAWGSHFDYCEGIRLFWN
                     DDKFVNAEILRVSL"
     gene            complement(3121..4320)
                     /locus_tag="VFQ23_03555"
     CDS             complement(3121..4320)
                     /locus_tag="VFQ23_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HET9905682.1"
                     /translation="MTDNLNEIQKRVNASREEMIQFMLEICEIPSMNSKIGPVGERVQ
                     AEMKKLGFDEVRFDKMGNTIGRIGNGDRVIVYDSHIDTVGVGDPSEWKWDPFKGKIED
                     GVLFARGACDEKGSTPGMIYGLAMARDLGLLDGWTAYYFGNMEEWCDGIAPNTFVEVD
                     PMVKPDFVVIGEPTKMLVYRGHKGRLEMKVTSKGKSAHAASNHLGDNAIYKLLPVIAG
                     IRDLEPQLGDHEFLGHGKITVSDMLVQTPSINAVPDEAVIFIDRRMTFGETKEEVRAQ
                     VDALIPDEYKNSVKLDELFYDEPSYTGFVFPVDKYFPAWALEQDHPLVLAGQQAREQI
                     GLPQAASGKWNFSTNGIYWAGKAGIPSIGFGPGDEETAHTVNDSVSLEDMVKATEFYA
                     ILPSLIK"
     gene            complement(4370..4813)
                     /locus_tag="VFQ23_03560"
     CDS             complement(4370..4813)
                     /locus_tag="VFQ23_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9905683.1"
                     /translation="MSGLNYPVKHIPSDMPENAITERIIKCAIAVHRELGPGLLEYVY
                     EEAMDIECKIDGLYVLRQFKVPVIYRDKIIGEYKPDMLINDMVIVEIKSVERFDPVFE
                     AQVLTYLRITQKRVGLLINFNSRLVKDGIKRLILSSNSQTTRDKK"
     gene            complement(4872..5588)
                     /locus_tag="VFQ23_03565"
     CDS             complement(4872..5588)
                     /locus_tag="VFQ23_03565"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9905684.1"
                     /translation="MANFPFQRLQADLAALIANTPPGQRLTSEPDLAKQLGVSRATLR
                     EAMRTFETQGLIRRRQGSGTYVVGAIPVIDAGLEVLESLDTMARRMNLDIQVSDLNVE
                     QVQADKETAAGLDIPEGALLTRVRRVIRADGRPAAYLIDTLPESIFRPSDLPAEFHGS
                     VLDFLIKRGDKLGVSRAGISATNAPSDVAKPLEIQRDDVLLKFVSKLYDTGGKVVDYS
                     VSYFIPGYFRFHVNRRVGNM"
     gene            complement(5588..7405)
                     /gene="ade"
                     /locus_tag="VFQ23_03570"
     CDS             complement(5588..7405)
                     /gene="ade"
                     /locus_tag="VFQ23_03570"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HET9905685.1"
                     /translation="MTSSTKLTRSLVDVAMGRVPADLVILNGNWVSVQSGEIIPGTDI
                     AIKNGHIAYVGEDASHTIGRKTEVIDAMGRYLVPGLLDGHMHVESGMVTVTEFVRAVA
                     VRGTTGMFIDPHEIANVFGLKGVKLMVDEAQKQPIHVWVQMPSCVPSAPGLETPGAHI
                     GPKEVAKAMKWKGIIGLGEMMNFPGVFMGDGNMLDEMSITHAHNKTIGGHYASPDLGI
                     PFHGYVAGGAEDDHEGTRLEDAVKRVRQGMKAMLRYGSAWFDVASQIGAITQHGLDPR
                     HFILVTDDSHAQTLAHDGHMDRVLRHTIEQGVNPVTALQMMTINTAEHFGVSKEMGMI
                     APGRWADLVLVKDLLDFRADLVVAKGRVIAEDRKWKFDLPKFTYPKWATNSVHLKRKL
                     KAEDFKLEAPSRTERSGSGDEAGKGLRANVIGVIENQAPTQHLTFDVRPRNGEIKVEM
                     KRDLAKIALVERHKGTGGITMGLVSGFGFTSKCAIASTVAHDSHHMIIVGTDEESMAV
                     AGNSLARSGGGQVVVKNGKVIGQVELKIAGLMSNEKADVVAKKAETILKGFRSCGCTL
                     NNPNMQLSLLALVVIPELRISDKGLVDVTKFNFVPVVEG"
     gene            complement(7435..8004)
                     /locus_tag="VFQ23_03575"
     CDS             complement(7435..8004)
                     /locus_tag="VFQ23_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9905686.1"
                     /translation="MNRLVIFSGLPGTGKSTLANRLARELRWPLLCIDDVVGEVPENT
                     GIPFWDSKVKILLRLTEVQLDLGLNVIVDSVFMNMDRHHAQELARKYDARFLPIYVHV
                     SDDRVWEQRVTARYDEMNNKDVATWERIQHQREHFRDWEPGTALFIDSLHSVDQNYEM
                     ILNFVAQETASTFLKPISAVPLVEGKYHA"
     gene            complement(8001..8312)
                     /locus_tag="VFQ23_03580"
     CDS             complement(8001..8312)
                     /locus_tag="VFQ23_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905687.1"
                     /translation="MLDFLLIALVGWPAIVGTIILALIGLIRRDYRFLVAAAILAFPF
                     AWYLSGFPIIRSPAFLLPLLLFTSGFAMNRGREMIAWLLTIPYFLSIILLLFAVLAGN
                     P"
BASE COUNT         2099 a         2323 c         1960 g         2119 t
ORIGIN      
        1 ttatattgtc tgcaccacga agcagaggcg cgcacgtcat cggcaagttc attgacgtcc
       61 actaacggac gatggaattg acagtgtaaa ccaagctcat gccctgccgc aacgattttt
      121 tgagggacct caggatacca ctccgcgatt tcacccacaa gatagatact tactttgcta
      181 tccttcaact tctcaagaat agggtcaacc gcctccaatg tgaatctggc atccagatcg
      241 cggcgagtct ttacatccgc aatctggtca taatggcgaa ataatgcaaa aacaggctca
      301 tagtcgatac ttaacagaac acgcgggcta atgggcataa gacgccaata ttaatggctg
      361 gatataggtg tgtcaagcac ggatgaaaaa ggactggttg tagaaccagt cctttggatg
      421 ccgctaactc gaaatccacg tcccagtttc acccttgagc gcccgcccga tattttcagg
      481 acatgtgatc agcacttgct tgcctccatt ttcgagatac cagatggccg cctggatttt
      541 cggtgccatt gagccttttg cgaagtggat tccctcttcc agataagcct tcgcttcggc
      601 tacattaata ttatccagcc atttctgatc tggcttgccg aaattgatgg caaccttgtc
      661 aaccgccgta gcaatgagga acatatctgc tttgagtgaa cgcgcaagta gagaggaagc
      721 gaaatcctta tcaatgactg ccgcggtccc ctccagttcg ccattcccgg catcaatcac
      781 cgggatgccg ccaccaccca ccgtgataac gatgtggcct gcgtccagca gcaatttgac
      841 cgcctcgaac tcgacgattt cttttggcaa aggggatgga acgactcttc tccagccacg
      901 gccggcatcc tcggcgacag accaccccat ctcctgttcg cgtcgttttg cttctgtttc
      961 gtccatgaag ctgccaattg gcttgctcgg cttttgaaaa gccggatcat tctgatcaac
     1021 ccgaacctgt gtgacaatcg tgcaagcctt tttcttgatg cctcgtctgt ggaactcgtt
     1081 ctgcagcgcc tgttgcaact catagccaat cgccccctgt gagtctgcac cgcatacgtc
     1141 caacgggact tcatgcatcc cttcggcttt cgctgcaatt tcagaacgac gcaggatgaa
     1201 cccgacctga ggaccattgc cgtgaccaat ggccaaatcc caaccggcat cgatcatatt
     1261 cacgagatgc ctggcggttt cctgcaacgc ctgcatctgg ctttcaacgg ttaccttttt
     1321 attgtccttg attaaagagt tgccgccaat cgcgacgact gcgagtttag tcatatggtc
     1381 tcctagtctt atagtgcaat agtcttgtag tcattcggtc atacaccaac aaactgactg
     1441 actatccgac gatgtgacca acaaactatt cgattagctc atcgtcaacg ccattactgc
     1501 tttctgtgca tgcaggcgat tctcagcctc atcgtaaaca actgaatgcc tgccatcaat
     1561 cacaccatcg gtcacttcga gtccgcggtc ggcggggaga cagtgcatgt agatggaatc
     1621 ctcttttgtg agatccatac ggcgctcgtc ggtgatccag gatttgtatt tatcaatcag
     1681 ggctttacct tcttctttgc cagtcgtggt caacaacgga ccccaggatt ttggatacac
     1741 gacgtcagcg tctttgaaag cttcatccat attatcggtg acatcaaaac cgacgccgta
     1801 tttcttcgcg ttatcctgtg cctgctgcat gatatcaggc atcaacttaa actccggcgg
     1861 atgcgccaga accacgtcac aaccaaagcg agtcatttgc agaatcaatg attgaggtac
     1921 agagatcggc ttgctgtagg aggcagcata cgcccaggag accacaacct tcttgcgttt
     1981 taggtcacgc cctttctttt caatgactgt cataatatct gccagacatt gaaagggatg
     2041 gtagacatcg cactgcatgt tcaacaccgg aacgcggctg gctttggcta cgtcgttgat
     2101 atattgattg ccaaattgcc agtcacactg acggatcgca atcccatcaa aataacgtcc
     2161 aaagatttca ccgatctcct tggcagtatc accgtgtgag atctgagttg tctcgctgtc
     2221 gataaaggcc gcgtgaccac ccaactgcgc catgcctgct tcaaacgatc cgcgcgtacg
     2281 ggtggaggta aagaaaaata acatcgccaa tactttgtca cgcaacagtg catggggttc
     2341 actcaatgca cgtttgcgtt tcagatccca cgctacgtcc aatacggttt cgacttcttc
     2401 tttcgagaag tccaggtcgc cgatgaaatc gcgccctcga aaattagttt gcattattag
     2461 tctcctttgt tttaaggtgg tggtcaagac gcaagcagtc gagaccacct cataaatttg
     2521 tattgtttac atttaattgg tggtttcgat atgccctcgc gaagagctct cgggctactc
     2581 aaccaccagg gataattaat tatagagata ctcttaatat ttctgcattt acaaatttgt
     2641 catcattcca aaacaaacgt atcccttcac aataatcaaa atgggatccc catgcaagat
     2701 gatttgcatc cggcaggcat gccaccgccc ccatttccaa aattccacca tgattgatga
     2761 caagcgcagc atgatcatcc gaaacatagt gtaatagttt gcgataaacg tctgctaatt
     2821 gattggcata tctggcggcg gcacctccct gactcacaac ctccgcatac ccggccagag
     2881 acagaggcca gggagcttct cgccctacgt cacgtccgta tgtactcatc aactcaattt
     2941 gctcatccac agcaaacccc atagcaatcg ccgtttcaaa ggcgcgaggc agcgtgcttg
     3001 ttactacaag atcaaacggg ccaatgtttt gaccgactcg ccgcgccaga gtcacacctg
     3061 tctgattcaa atgatcacca ggctgatggc gaatggagtg gcggcggatt tcgatcgctt
     3121 tcatttgatc aggcttggga ggatcgcgta gaattctgtt gctttgacca tgtcttccag
     3181 tgagacgcta tcgttgacgg tatgcgcggt ctcttcatca cctggaccga aaccaatgga
     3241 aggaatccca gctttgcctg cccagtagat accgttcgtg gaaaagttcc acttgccgct
     3301 ggcagcctga ggcaaaccga tttgttcacg cgcctgttgc cctgctaaga cgagcggatg
     3361 gtcttgctcc aaggcccacg ctgggaaata tttatccacc gggaaaacga atccggtata
     3421 ggagggctcg tcgtaaaaga gttcatccag cttgacactg tttttgtatt catcggggat
     3481 gagcgcgtca acctgtgccc ggacctcttc ctttgtttcc ccaaatgtca tgcggcgatc
     3541 gatgaaaata acggcttcat caggcactgc attgatggat ggcgtttgca caagcatatc
     3601 actcacggta atcttgccat gacctaaaaa ttcatgatcc ccaagctgcg gttccaggtc
     3661 acggataccg gcgatcacgg gcaatagctt ataaatggca ttatctccaa gatgattcga
     3721 agctgcgtgc gccgatttgc ccttggaggt gaccttcatc tccagccgcc ccttgtgacc
     3781 tcgatagacg agcatcttgg taggctcacc gataacgaca aaatcaggct tgaccatcgg
     3841 atcgacttca acgaaggtgt tcggcgcaat accatcacac cactcttcca tattcccaaa
     3901 gtaataggcc gtccagccat ccagtaagcc caaatctcgc gccatcgcca ggccgtagat
     3961 catcccgggc gtggatccct tttcatcaca cgcgccgcgc gcaaagagaa caccatcttc
     4021 gatcttgcct ttgaatggat cccacttcca ctctgaaggg tcaccaacac cgacggtatc
     4081 gatatgggaa tcatagacaa tcacacgatc cccatttcca atgcgcccaa tggtgtttcc
     4141 cattttgtca aaacgcactt catcaaaacc aagcttcttc atctcggctt gcacgcgctc
     4201 ccccacggga ccgatcttcg aattcatact cggaatctca caaatctcca gcatgaattg
     4261 aatcatctcc tcacgtgagg cattcacacg cttttgtatt tcatttaggt tatctgtcat
     4321 aaatactcct gatttatctg ccgctgagtc acagagacgc agagtctttt tatttcttgt
     4381 ctcgagtcgt ttggctgttt gacgaaagga ttaatcgttt tatcccatcc ttcacaaggc
     4441 gggaattgaa attgattagc aaaccaaccc gcttctgagt tattctcaga taagtcaaca
     4501 cctgtgcttc aaatacagga tcaaaccgtt ccacactctt aatttcaacg atgaccatat
     4561 cgttgatcaa catatccggt ttgtattctc caataatttt gtctcgatat ataaccggta
     4621 ctttaaattg tcttagcaca taaagcccgt caattttgca ttcgatgtcc atcgcttcct
     4681 cataaacata ttccagaaga cctggaccaa gttctcgatg caccgcaatc gcacatttga
     4741 tgatacgctc tgttatggca ttctcaggca tatcggaagg aatatgttta actggataat
     4801 ttagaccact catgattttg ctgtactggg aaaccaaaat ttctcggcgt ctccgcgtct
     4861 ctgtggcaga actacatatt cccaacgcgt cgattgacgt ggaagcggaa gtaccctgga
     4921 atgaagtagc tcaccgaata gtccacgact ttgccgcctg tgtcatacag ctttgagacg
     4981 aacttaagca atacatcatc gcgttggatt tcgaggggtt tcgccacatc ggatggtgcg
     5041 ttcgtggcgc tgatcccagc ccgagagact ccgagcttgt cgccgcgttt aatcaaaaaa
     5101 tccaggacag agccgtgaaa ttcggctggc aaatcacttg ggcggaatat cgattcaggc
     5161 agggtatcga tcaggtaagc cgccgggcgt ccatcagcac ggatcacgcg gcggacgcgt
     5221 gtcaggagtg cgccttcggg aatatccaag cctgcggccg tctctttgtc cgcctgcact
     5281 tgttccacgt tgaggtcact gacttgaata tccaaattca tgcgacgcgc catggtatca
     5341 aggctttcca gcacctcgag ccccgcgtca attaccggga ttgcccctac gacataagta
     5401 cccgaaccct ggcggcgccg gatcaggccc tgcgtttcga aagtacgcat cgcttcccgc
     5461 aaggtggcgc gagagacccc taattgcttt gcaagatcag gctcagaggt caggcgctga
     5521 ccaggcggcg tgttggcgat caaggcggcg agatctgcct gcagacgttg aaacggaaaa
     5581 ttagccatta tccctccact accggaacga aattgaattt ggtcacatcc accagacctt
     5641 tatctgagat tcgcaattct ggaatcacta ccagcgcgag cagtgacaac tgcatatttg
     5701 gattgttcaa cgtacagcca caggatcgaa aacccttaag tatggtttcg gctttctttg
     5761 cgacaacatc tgctttttca ttagacatca agccagcgat cttcaattcg acctgaccga
     5821 tgacttttcc attcttgaca acgacctgcc ctcccccact tcgcgcgaga ctattgcctg
     5881 ccaccgccat cgattcttca tccgtgccga cgatgatcat gtgatgcgaa tcatgggcaa
     5941 ccgtagaggc aatcgcgcat tttgatgtaa acccaaaccc gctgacgagc cccatcgtga
     6001 taccgcccgt tcctttgtga cgttcgacaa gggcgatctt tgcgaggtcg cgtttcatct
     6061 ctactttgat ttcaccgttt cgaggccgga cgtcgaaggt caggtgctga gttggggcct
     6121 gattctcaat cacgccaatg acgttggctc gaagcccttt gcccgcttcg tctccgctac
     6181 cactccgctc agtgcgagag ggagcttcta atttgaaatc ctcagctttc aattttctct
     6241 tcagatgcac agagttcgtt gcccatttgg gataggtgaa cttcggcaaa tcaaacttcc
     6301 attttcgatc ttcagcaatg acacgtccct tcgcaactac aaggtctgct cgaaaatcca
     6361 gcaaatcctt taccagcacc aaatctgccc agcgacccgg ggcgatcatg cccatttctt
     6421 tactcacacc aaagtgctca gcggtgttga tcgtcatcat ttgaagcgcg gtgacaggat
     6481 taactccctg ctctatggtg tgtcgtagga cacggtccat atgcccatca tgagcgagtg
     6541 tttgggcatg ggaatcgtcg gtgacgagga tgaagtggcg tgggtctaac ccgtgctgtg
     6601 tgatcgcacc gatttgtgag gcgacatcaa accaggcaga gccataacga agcatggcct
     6661 tcatgccctg acggactcgt ttcaccgcgt cttcgaggcg agtcccttcg tggtcatctt
     6721 ccgccccacc cgctacgtag ccatgaaagg gaattcccaa atctggcgag gcgtaatgcc
     6781 cgccgatggt cttgttgtgc gcgtgtgtga ttgacatttc atccagcatg ttcccatcgc
     6841 ccatgaacac gccggggaag ttcatcattt cgcctaagcc gatgatgcct ttccatttca
     6901 tcgcttttgc gacttctttg ggtccgatgt gcgcgccggg ggtttccagc ccgggagcgg
     6961 atggaacgca ggagggcatc tgcacccaca catggatggg ctgcttttgt gcctcatcca
     7021 ccattaattt tacgccctta agtccaaaga cattggcaat ctcatgcggg tcaatgaaca
     7081 tgccggttgt gccgcgcacc gccaccgccc gcacaaactc tgtgacggtc accatccccg
     7141 attcgacatg catatgcccg tctaacaaac ctggcaccag ataacgcccc atcgcatcga
     7201 tgacttcggt tttcctgccg atcgtgtgac tcgcatcttc acccacataa gcaatgtgtc
     7261 catttttgat ggcgatatcc gtaccgggga tgatctcacc cgattggaca gatacccaat
     7321 tgccattgag aatgaccaga tcagcaggga cacgccccat ggcaacatca acgagagagc
     7381 gagtgagttt tgtggaggag gtcatagggt ggttcctaat cgaagaacgc ccgattaagc
     7441 gtgatactta ccttcaacca aagggacagc agatatcggc ttgagaaaag tagaggcagt
     7501 ctcttgcgcc acaaaattta agatcatttc atagttttga tcgacagagt gtagagagtc
     7561 gataaacagg gcagtaccag gctcccagtc gcggaaatgc tcgcgttgat gttgaatccg
     7621 ttcccaggtg gctacatctt tgttgttcat ttcatcgtaa cgagccgtaa cgcgttgttc
     7681 ccagactcta tcgtccgata cgtgaacgta gatcggaaga aaacgcgcat catatttacg
     7741 agccagttcc tgggcatgat gacggtccat gttcataaaa accgagtcga caatgacatt
     7801 cagacccaga tccaactgta cttcagtgag ccttagcagt atcttcactt ttgaatccca
     7861 aaacgggatg ccggtgttct caggcacttc tcccacgaca tcatcaatac acaagagcgg
     7921 ccagcgtaac tcgcgggcaa gccgatttgc aagtgtggat tttcctgtgc cgggcaagcc
     7981 agagaatatg accagtctat tcatgggttg ccagccagta cggcaaagag caacagaatg
     8041 atggacagaa aatacggaat ggtcaacaac caggcgatca tctcacggcc acggttcatg
     8101 gcgaagccgg aggtaaagag cagcaaaggc aaaagaaacg ctggcgagcg aatgatcgga
     8161 aacccgctca ggtaccaggc aaatggaaat gctagaatgg cagcagcaac gagaaaacga
     8221 taatcacgcc tgatcaggcc aataagggca agaataatcg tgcccacgat cgcgggccac
     8281 ccaacgagag caatgaggag gaaatcaagc atattatatg aaacagcgtg gtttcgatac
     8341 ggcggcgctg gtcgagtagg acgatagtcc gtatcgagac ccgccgccta ctcaaccagc
     8401 ggattatgat aacaaccttt tcgctgcctt attatgtttc tccgctaact accgttcatc
     8461 aacagtgacc aactgtcctt ttttgaccac gaacttgcca c
//
LOCUS       DASTJP010000115        17307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_136046, whole genome shotgun sequence.
ACCESSION   DASTJP010000115 DASTJP010000000
VERSION     DASTJP010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17307
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136046"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFQ23_03585"
     CDS             complement(<1..615)
                     /locus_tag="VFQ23_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905688.1"
                     /translation="MNLLIRNGTLITDSDTFQSDILIQDEKIMLVAPGLEFDSFETMD
                     ASGKLIMPGGIDPHVHLDLPMFDTVSSDDHYTGHKAAAFGGTTTVMDFVPLTPTPLPQ
                     GEGRRGEGESPTGDFKYSIDLWLQKAQKAAIDYSFHMNLTQFNEKIAKQIPSLPEMGI
                     TTLKVFTAYNGRLRLDDGSIFKALKIARENGMLTMAHCENGDVIE"
     gene            complement(612..2837)
                     /locus_tag="VFQ23_03590"
     CDS             complement(612..2837)
                     /locus_tag="VFQ23_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9905689.1"
                     /translation="MSLRQILPQRLHNRNWTLLAGLVLVGLMTLIAIIGPELAPQDPM
                     KENYTLAVDGQIRTPPYPAFKIDGYPLGTDRWGRDLLSRILWGVRPTLIMVASVAAFR
                     LAVGIILGLLIGWAEGRRARQLDSILSSLLSIPVLIVALIGIYAIGVEKGLWAFIFGL
                     GITGWAETARMVSEQTRVVKRQTFVEAARALGSAERQILVNHILRQIMSLVWVLLAFE
                     ISSTLLVAAGLGFLGYFIGGGIWIEVTDFQAVNVEGLPELGQMLSSALVRITDPTALV
                     IIGTIIFAGVLGFNLLGEGLRIELTQTELGRRIRFVPHAITEWYEVQFQPAVRQWSEV
                     HGKSVGRIAFVAFLLIGGWIYYDRNSYRFVETETPIQTTNSHLWATELHDAYGTLWVP
                     ISMETMPELKWEVQLPGGASGGPAVSMDGTVYAAGVDNRLMAFNPQGELLWESPLDAA
                     PIGAPALDAQGRIFVADFDGNLTAFDIQGNRLWRVEASATRQSTSGPVVASSGMIYLT
                     LIDAVVGISPEGTLVWRVTATDEYVDSPPRLSADERLVFLKSTAMDARTGQIQAIQIR
                     PENEVLFTEPAFFTGVDGRNYYRNGHEMMMWQLTETGPQVEEPVSWEAQSFVLFNPVT
                     QGVTSNGLAWMFYGSDFNDGRVVWIDEQSRLVGNYRFPHGSSRLIAMGGNGEAYLCGP
                     TGARLECVSAMPGSEEPLWSIFIENSAPPIGGALVPGTLYVTTEDGTLYALSTEGNTQ
                     P"
     gene            complement(2834..3796)
                     /locus_tag="VFQ23_03595"
     CDS             complement(2834..3796)
                     /locus_tag="VFQ23_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905690.1"
                     /translation="MISFVVRRLLTFPFIVLAANFIGFAFAFYFEPVVATSNPYSSGN
                     IQLPPLFPEYFAYLSKFAQLDFGETYNGEPVLAAISRLGLISLGLVAIALGLSIVLGI
                     FLGRLAVKPDSIKVSSWLTVLATVGLASPGFYIAILLITFFLLLTLYGPGNDLLIPFQ
                     GFGWDSHLILPVMVLMIQPTVKIAQVTGSVLNDELQKPYVKAGISLGLTFESMKGRFA
                     FRNVFAPVLQAIASSARLMVAELIIIERLFNWPGLGRFIGNVLEPNRGFFNAVVLTPS
                     TMAALLSVLVMIFLIIDFVTIFIARLVDPRLRMDLFTEAQGAAR"
     gene            complement(3800..4354)
                     /locus_tag="VFQ23_03600"
     CDS             complement(3800..4354)
                     /locus_tag="VFQ23_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905691.1"
                     /translation="MPISPTSLILVCLLPSPRDLEIARLLGWYRIPLRTAPKVVAVDY
                     LAFYQPSAFGDRGGKIEFIAPVRGHELTTRGELLKDEADHPRAREEYYKIQLGRLEKL
                     PQSIEADKWKRLTFLYTTGEYLLNAKLLNDLVVQNEERDILWKSLRERAENAQLYQID
                     LPDADVDPALLMLLLGISGMEKDA"
     gene            complement(4433..5899)
                     /locus_tag="VFQ23_03605"
     CDS             complement(4433..5899)
                     /locus_tag="VFQ23_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9905692.1"
                     /translation="MTTIDLTIHKDRRARAIRRAREKNIIIPTYSQMKDPSKIPSGVK
                     NELKDIGLWDIHPRNLFRINWHNSPSASGGTYGGVNFLELPSSLTGVPARIFVIIGKW
                     FPTGAHKVGAAFSCLVPRLVTGQFDPTTQKAVWPSTGNYCRGGAYDSALLGCESIAIL
                     PEGMSKERFEWLSKVAGETIKTPGSESNVKEIFDKCWELRKSGQDLMIFNQFEEFGNY
                     LWHYEVTGHAMEEVLQQVMGPNDHYRGMASATGSAGTIASGDYLKQWFPDSKIVASEA
                     LQCPTLLENGFGAHRIEGIGDKHVPWIHNVKNTDIVTAIDDNAVVSLARLFNEENGRA
                     YLVEKGVPESLISNLDLLGFSGISNVLSCIKTAKYYEMTENDIMLTVLTDSMELYRSR
                     LHEMHQEFGEYTERDAAADFARHLHGQSTDNMLELRYPDRRRVHNLKYYTWVEQQGRT
                     YEEIQQQWYEPDYWTNVQKQADEIDDLIIEFNNEVGLS"
     gene            complement(6064..6459)
                     /locus_tag="VFQ23_03610"
     CDS             complement(6064..6459)
                     /locus_tag="VFQ23_03610"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9905693.1"
                     /translation="MMKLIVFSGLPGTGKSTLAESLAKTLGIPVFAKDWLEASLLRSE
                     LTPSIAEKPLGFAGYDLLTTLAQRQLKLEQSVILDSVASTETVRKAWRALADQYKAGW
                     RVIECICTDESLHRSRLQLRERRIPAGTN"
     gene            complement(6747..7907)
                     /locus_tag="VFQ23_03615"
     CDS             complement(6747..7907)
                     /locus_tag="VFQ23_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HET9905694.1"
                     /translation="MPYNFDDVPNRRTTQLRNKWTWYPNDVLPMWVADMDFQSPKPIR
                     DALHHYLDLGVMGYESPSKQLLEAVAERMHRLYRWKISEEMIVTLTGVNIGYNVAART
                     FCRSRKQYLIQTPVYNEFHDTEKKTGVREIESPLVKIVEGNRIRYEVDFDAFGKAARK
                     ADLFLLCHPHNPIGHIYSPQELKQIAEICIKNDLIIVSDEIHSELLLGNATFQPMASL
                     AREMANRTITLISASKTFNVPGLFCAFAIIPNKQLREAYKETVFNMGIHISSAGLVAA
                     RVAYEGQCDSWLRSLRRYLTANRDWLVDAVTRHLPEVHITVPDSTYLAWLDFSAFKLK
                     PSPFRFFLKEAKVALSDGRKFGKGSEQFVRINFGTSRKWLEEGFKRIKKALK"
     gene            complement(7985..8623)
                     /locus_tag="VFQ23_03620"
     CDS             complement(7985..8623)
                     /locus_tag="VFQ23_03620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET9905695.1"
                     /translation="MAEISYDKVQDYITSLVPAREPELQEMEAYAEKNGFPIIGASCG
                     YYLYQLARMLNATSVFELGSGYGYSTAWFAKAVIENGGGVVHHTVWDEDLSKRAQGHL
                     SRLGRSEVVKYHNAEAVETLRQTDGPFDIIFNDIDKEGYPDSLPVIKAKLRNGGILII
                     DNMLWSGRPFDPKNQEQSTEAIRRFTQNITTDPDWIVSLTPMRDGMIVAYKK"
     gene            complement(8645..9352)
                     /locus_tag="VFQ23_03625"
     CDS             complement(8645..9352)
                     /locus_tag="VFQ23_03625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9905696.1"
                     /translation="MRSFFLCWLLLLTACTQNASKTIIGDDVMPDVRFLALGDSYTIG
                     ESVPEAERWPNQLAELIRSSPPFDETGGSIEVTIIARTGWTTDELWNGIQEVTIEPPY
                     DLVSLLIGVNNQYRGRDIEEYRAGFVFLLNKAIAYAGGDPGRVIVLSIPDWGVTPFAK
                     DRDSKQIALEINQFNAVNEEESKKAGVHYVDVTPISREAATDVSLIASDGLHPSGKMY
                     AEWAKLALPDALRIIEQ"
     gene            complement(9481..10248)
                     /locus_tag="VFQ23_03630"
     CDS             complement(9481..10248)
                     /locus_tag="VFQ23_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905697.1"
                     /translation="MSMDTIRQFANLFSVILALTINILASTLPLNGQNTGEISDRFEV
                     YFVPAGYVFAIWGIIYIGWIAFTIFQFQRSQKESPRLRRLGYLFALSNLANAGWLFCW
                     HYNRFGLSVLIMLILLGMLIASYLQLNVNRISVTRVEYWSVDVPFGLYLGWITVATVA
                     NIADWLYFVEWNGFGVSAQTWAVIMLAVASLLGLAMALTRRDAAYLVVLVWAFVGIAV
                     KQVSAPLVVLSSWIAAIVMLGLVIFSLTRPQPAKSKI"
     gene            complement(10331..11266)
                     /locus_tag="VFQ23_03635"
     CDS             complement(10331..11266)
                     /locus_tag="VFQ23_03635"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET9905698.1"
                     /translation="MQVHANWENQLLALAGNTPHPSTRPLFSYWAGDAALSSAYKQAE
                     QITSEHSKSFYFASALLPEEKRSAVRALYAFCRVVDDIVDESTDIEREEKLNYWREMV
                     ETASFSEDDLVAAAWADTIIRYHIPRHYALQLIDGVARDLTQSRYQTFEELTTYCYGV
                     ASTVGLMSMYIIGFHSNEAVPYAIKLGVALQMTNILRDVGEDHQIGRLYLPREEMAFY
                     GVNEQDIAAGRVTNHWRQFMKFQIDRTRQLYDASWEGVKMLEREGQLAIGAASVFYQG
                     ILDEIEKNDYDVFTRRASLSSWGKARRIPALLQRL"
     gene            11435..12973
                     /gene="crtI"
                     /locus_tag="VFQ23_03640"
     CDS             11435..12973
                     /gene="crtI"
                     /locus_tag="VFQ23_03640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HET9905699.1"
                     /translation="MNKPTAIVIGAGIGGIATAARLAKNGYDVTVLEKNELPGGRCNQ
                     IVHNGHRFDIGPTLFLMPEVWEETFASLGEKMSDHLDLRRIDPTYKVHFDDGLKLELT
                     SNIGTMQTQLEKVEKTAFSGFLNYLAEGNKHYKISLEKFVGRNFYTIFEYLSLANLPL
                     MFKLKALAKHHANTGRYFKDERLKAAFTFQNMYLGLSPYDAPATYSLLQYTELAEGVW
                     YPIGGMYAAIQALVRIAEKLGVRFSYNAPVRKLVTNKNKVLGVELENGQSLVADIVIG
                     NADLPYIYKNLLPDKAEAKKLEKKLYTCSTIMFYWGVDKEYPQIAHHNVFLGGDYKAS
                     FDQIFENHTLPDVPSFYVHAPARTDEKAAPQGQDTLYALIPVGHLDPHQKQDWDALVN
                     RARETVFDRLAKELGVTDLKEHIKFEIVYDPEVWQDRFNLARGAAFGLSHNFWQVGYL
                     RPQNRHKKYKNLYFTGASTHPGTGLPIVLLSARLTTERILKEMSTIAVQKAVNPVLNT
                     AQGN"
     gene            12974..14320
                     /locus_tag="VFQ23_03645"
     CDS             12974..14320
                     /locus_tag="VFQ23_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HET9905700.1"
                     /translation="MVSTTPAFNLKVVLKETGLAADTLRAWERRYGLPVPQRTRGGHR
                     LYSQRDIETIKWLMKRQTEGLSISRAVDMWNEQLAAGSDPLADLIDSLLASASPVLSQ
                     NRTPDTTLDLLRSQWVEACIEFNESKAEQTLNQAFSMFPVEVVCLDVLQRGLSEIGDL
                     WQENQVSVQQEHFASGLAMRRLDALLISTPPPTRNQILLIGCPPGEWHVFTPLLFALF
                     LRRRGFPLIYLGGNVPAPRFADTVKNVKADLVILIAQQLVTAATLQETVFLLAKQKIS
                     VAFGGRIFNVQPEITNSISGYFLGTELIAALDEIELILRSKATHRQQKTASQEYITAH
                     QSFTSKRTQIELTLKETLEPNSISPEDVKTGIHFLGDNITAALQLGDMSYVSAEVDWL
                     KNLLQAYETPSQQLIHFMKTYARSLDQAMDGQGKPIFEWFAAEIQKLDNDGLVEDY"
     gene            14320..15336
                     /locus_tag="VFQ23_03650"
     CDS             14320..15336
                     /locus_tag="VFQ23_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905701.1"
                     /translation="MQAAKTNPLLRYLLPGILVSAAFTVIIGLTAANLTGFQIVGDTD
                     ILFNYPWQLREPTALARLTAWTGYLIHNLIVWAIIYFAQREKPKFGNDLRWFNWAMIW
                     THVTFIVLHIVQTQLFYDGLAQDVPEVTAQGSVALMLIIIILFETPRRGLIFGKKFKF
                     KDGFMYIARKYHGYFFAWATTYTFWYHPTEGTWGHLMGFLYMFMLFVQSALIFNRVHL
                     NKWWTFTLEVFVVIHGTVVAVLQGNGIWPMFAFGFGAMIVLTQMYGLGLNTWTKRALA
                     IGFVLMIVAYYTLFDSIAGLNEVIRIPVIDYVVIFLLYGIYLFINWLVNIFKRPTPMQ
                     AGTD"
     gene            15351..16199
                     /locus_tag="VFQ23_03655"
     CDS             15351..16199
                     /locus_tag="VFQ23_03655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9905702.1"
                     /translation="MKDKIVVLTGSTRGFGYAIAEAMLKAGATVIVSGRSEEVLAKAT
                     ASLRSHGDVKGQICDVREKLQVYALARFAIQSFGRIDVWINNVGYSSAAGEMLETPPE
                     QAIDMFLANDMGTLYGSQAALHYMSQQRQGTLVNVYGNGSFLRPASPTGLYGATKAWV
                     TSFTRSLAKEIKGGGVNLIGFSPGMMMTDMLTNPVVIGERGKEMMKRYSFVLRFLGDD
                     PTKAAQQLVETIANNKKEFIEYQMLRPWTITFRLLRVMWENLTKTGQTPQYTLHYEEA
                     YKPDIK"
     gene            16196..>17307
                     /locus_tag="VFQ23_03660"
     CDS             16196..>17307
                     /locus_tag="VFQ23_03660"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905703.1"
                     /translation="MNNKYLFGIILVVFIDLLGFSLIIPLLPYYAQTFNASDTTIGLL
                     LASYAAAQLIGAPLLGRASDQFGRRPILLVSIFGTFLGFLLFGLANSLTMLFASRILQ
                     GITGGNLSVAQAYITDVTDAQSRNRGLGMIGAAFGLGFIIGPALGGVLSNISYSVPAF
                     VAAGLSFINLILIIFWLPESLTPERRAQLSPKRSGITLQALLESLRRPLTGTLLITRF
                     LYSLAFVILQSIFSLFALRRFGMSVVATGFVLTYVGVVSVVTQAWLIGKLSQRFEDMV
                     LIQFGLVLLGIGLLTWAFAPNIPFLVLSVTPIALAGGMLNTILPSSLTKTVEPQDVGG
                     ILGLSTSIESSTRVVAPLLGGFMLDNISYWAPGLFGT"
BASE COUNT         4432 a         4320 c         4155 g         4400 t
ORIGIN      
        1 ttcgatgaca tcaccgtttt cgcagtgtgc catcgtgagc attccgtttt ctcttgcgat
       61 tttcaaggcc ttgaagatac tgccatcatc caggcgtaaa cgtccgttgt aggcagtgaa
      121 tactttgagc gttgtgattc ccatttcagg cagggacgga atctgtttcg cgatcttctc
      181 attgaattgg gttaggttca tgtggaagga ataatcgatc gcggcttttt gtgctttctg
      241 gagccagagg tcaatcgagt atttgaagtc tcccgtggga ctctcgccct caccccttcg
      301 cccctctccc tgagggagag gggttggggt gagcggcaca aaatccataa ctgtcgttgt
      361 tccgccgaac gccgcggctt tgtgaccggt gtaatggtca tcggaagaaa cagtatcaaa
      421 catgggcagg tcgagatgca catgggggtc aatgccaccc ggcataatca gcttacccga
      481 cgcgtccatt gtttcaaacg aatcaaattc aagtccagga gcgacgagca tgatcttttc
      541 atcctggatc aaaatatccg attgaaatgt atcggagtcc gtgatgagtg tgccattcct
      601 gattagaaga ttcatggctg tgtattgcct tctgttgaaa gcgcgtataa agtgccgtcc
      661 tccgtagtga catacaaggt gccaggcacg agtgctccgc caatgggtgg tgccgagttt
      721 tctataaaga tgctccaaag aggttcttct gagccaggca tcgctgacac acattcgagg
      781 cgcgctcctg tgggtccgca cagataggct tcgccatttc cacccatagc gattaaacgt
      841 gaggatccat gcgggaagcg atagttaccg acgagtcgac tctgttcatc tatccacacc
      901 acgcgcccat cgttgaagtc tgagccatag aacatccatg ccaacccatt ggacgtcaca
      961 ccttgagtaa ccgggttgaa taaaacgaac gattgagctt cccaactgac cggttcttcc
     1021 acttgaggtc cggtttcggt cagttgccac atcatcattt cgtggccgtt acgatagtaa
     1081 tttcttccgt ctacgccggt gaagaaagca ggctcggtga aaaggacctc attctcaggc
     1141 ctgatctgaa tggcttgaat ttggcctgtg cgagcatcca tagcagtgct tttgagaaat
     1201 accaggcgct catccgcact gaggcgcggc ggcgaatcca catattcatc tgtggcagtc
     1261 acacgccaga ccagggtgcc ttcgggcgaa atgcccacca ccgcatcgat caacgtcaga
     1321 tagatcatcc cgctagaagc taccactggg cctgaggtgg actgtcgggt ggcagaagcc
     1381 tccacacgcc agagccggtt gccctggatg tcgaacgccg tgagattgcc atcaaaatct
     1441 gcaacaaaga ttcttccctg tgcatccaaa gcaggcgcgc cgatcggggc tgcatcaagc
     1501 ggggattccc acagcagctc tccctgcgga ttgaacgcca tcagcctatt atccacccct
     1561 gcagcataaa cggtaccatc catagaaact gcgggtccac cggatgcccc gccaggcaac
     1621 tgcacttccc atttcaactc aggcatagtt tccatgctga tcggcaccca taatgtgcca
     1681 taagcatcat gcagttcagt tgcccagaga tggctgtttg tagtctgtat cggtgtttca
     1741 gtttcgacaa agcgataact gttacggtca tagtagatcc atccaccgat gagtaagaat
     1801 gccacgaacg caatcctccc gactgacttg ccgtggactt cactccattg tcttacggct
     1861 ggttggaatt gaacttcgta ccattctgtg atggcatgag gcacaaatcg aatgcgccgg
     1921 cccaattctg tttgggtaag ctcaattctc agcccttccc caagaagatt gaatcccagc
     1981 acccctgcaa atatgatcgt cccgatgatg actagcgcag ttggatcagt gatacgcacc
     2041 agtgcagagg acagcatttg tcccagctca ggcagccctt ccacattgac cgcctgaaag
     2101 tcagttactt cgatccaaat gcccccgcca atgaaataac cgagaaagcc caatccagct
     2161 gcaaccaaca gtgtgctgct gatctcaaac gccagcaata cccaaaccag cgacatgatc
     2221 tgacgcaaga tgtgattgac caggatttga cgctcagcgg accccagcgc gcgcgcggct
     2281 tcaacaaacg tctgccgttt cacaacacgg gtctgttcgc tgaccattcg ggctgtctct
     2341 gcccagccgg tgattccgag cccaaagatg aaggcccaca atcccttttc cacgccaatt
     2401 gcataaatgc cgatcaacgc gacgatcaat acgggaatcg aaagtaagga agaaaggatc
     2461 gaatcaagct gtcgcgctct ccgaccttct gcccagccga tcagcaagcc aaggatgatt
     2521 cccacggcca gacgaaacgc cgcaaccgaa gcaaccataa tgagcgtcgg tcttacgccc
     2581 cacaatattc gactcaacag atcgcgtccc cagcgatccg tccccagcgg ataaccatct
     2641 atcttgaatg cggggtaagg cggggtgcgg atctggccat ccacagctaa ggtgtaattt
     2701 tccttcatcg gatcctgtgg agccagctct ggtccaatga tggcgatcag agtcattaac
     2761 ccaaccaaaa ccagaccggc aagtaaagtc cagtttcgat tgtgcagtcg ctgtgggagg
     2821 atttgtctta gactcatctt gctgctccct gcgcttccgt aaatagatcc attcgcaaac
     2881 ggggatcaac cagacgagca atgaagatcg taacaaaatc aatgatcaga aatatcatca
     2941 cgaggactga taacaatgca gccatcgttg atggagtaag cacaactgca ttaaagaagc
     3001 ctctgttcgg ttcaagtaca ttgccgataa aacgcccgag acctggccaa ttgaacaatc
     3061 gctcgatgat gatcaactca gcgaccatca agcgtgccga agaagcaatc gcttgtaaga
     3121 ctggagcaaa aacatttcga aacgcaaagc gtcccttcat ggattcgaag gtcagcccga
     3181 ggctaatccc agcttttaca taaggtttct gcaactcatc gttgagcaca ctgccagtca
     3241 cctgagcgat ctttacggta ggttgaatca tcaacaccat caccggcaaa atcaggtgag
     3301 agtcccagcc aaacccctga aatggaatga gtaaatcatt tccgggccca tataacgtta
     3361 gcaaaagaaa aaatgtgatc agtaatatgg caatgtaaaa acccggggaa gccagcccta
     3421 ccgttgcaag cacagtaagc caggaagaaa ctttgatgct atcaggcttt actgccagac
     3481 gaccaaggaa aatccccagc acgatgctta atccgagcgc aattgcaacc aatcccaaac
     3541 tgatcaatcc aaggcggctg atggctgcca gaacaggctc accgttgtag gtctctccga
     3601 aatcaagctg tgcaaatttt gatagatacg caaaatactc agggaagagc ggaggtaatt
     3661 gtatattgcc actggaatac ggattgctcg tggcaaccac gggttcaaaa taaaacgcaa
     3721 aagcaaagcc aatgaaattt gcagccagca caatgaacgg aaaagtcaaa agccgacgca
     3781 cgacgaagct gatcatgaac taagcatcct tctccattcc gctaattccc aacaagagca
     3841 tcaagagtgc agggtccaca tctgcatccg gaaggtcgat ctgatagagc tgtgcatttt
     3901 ccgcgcgttc tcgcaaactc ttccacaata tatctcgctc ttcattttgg acgaccaggt
     3961 cattgagcaa cttggcattc agcaaatatt cgccggttgt atagaggaaa gtcagacgtt
     4021 tccatttatc cgcttcaatg gattgaggta atttttccag tctgcctaat tgaattttgt
     4081 aatattcttc ccgggcacgc gggtgatccg cttcgtcctt cagcaattcg ccacgtgtgg
     4141 tcaattcatg tccgcgtacg ggcgcaatga actcaatctt cccgccccgg tcgccaaatg
     4201 ccgatggctg gtaaaacgcc agatagtcca ctgccaccac tttaggcgcc gtacgaagcg
     4261 ggatgcgata ccagcctaaa agccgcgcga tttcgaggtc gcgtggggat ggcagcaggc
     4321 agacaaggat cagtgaagtg ggagaaatgg gcatatctga aatagaaagt ggaaagatgc
     4381 aagttgattc tacccgctac tttccacgtt ttagcagatg gttaagtaga ttttagctca
     4441 atccgacctc attattgaat tcaatgatca gatcatcaat ctcgtctgcc tgtttttgga
     4501 cgtttgtcca atagtctggc tcataccatt gctgctgaat ctcttcatag gtgcgaccct
     4561 gttgttccac ccaggtatag tatttgagat tgtgaacgcg gcgtctatca ggataacgaa
     4621 gctcaagcat attatctgtt gattgaccat gtaaatggcg tgcgaaatca gcggcagcgt
     4681 cgcgctcggt gtattcacca aattcctgat gcatttcatg caagcgagaa cgatatagct
     4741 ccatagagtc ggtaaggacg gtgagcatga tatcgttttc ggtcatctcg taatatttgg
     4801 ccgttttaat gcaggagaga acatttgaaa ttccggagaa acctaataga tcaagattgg
     4861 agatcaggga ctcgggaaca cccttttcga cgagatacgc ccgtccgttt tcttcgttga
     4921 acagacgggc caggctgaca acggcgttgt catcaatggc tgtgacgata tcggtgttct
     4981 ttacattgtg aatccagggt acatgcttat cgccgatccc ctcaatgcgg tgcgcaccga
     5041 atccgttttc gagcagggtg gggcactgca aggcttcgct ggcgacgatc ttgctgtcgg
     5101 ggaaccactg cttaagatag tcgccgctgg caattgttcc ggctgacccg gtggcggacg
     5161 ccatcccccg atagtgatca tttggtccca ttacttgctg caacacttct tccatcgcgt
     5221 gaccagtcac ttcataatgc cagagataat tcccgaactc ctcgaactga ttgaagatca
     5281 tcaggtcctg cccggatttt cttaattccc agcacttatc aaaaatctct ttgacgtttg
     5341 attcagatcc aggcgtcttg atcgtctcgc cggcgacttt ggataaccac tcaaaccgtt
     5401 ccttagacat cccctccggc aaaatcgcaa tggattcaca tccgaggagc gcggaatcat
     5461 acgcaccacc gcggcaataa ttcccagtgg atggccagac ggccttttgc gtggtcgggt
     5521 cgaactgacc ggtgaccagc cgcggcacca ggcagctgaa tgcggctccc actttatgcg
     5581 caccagtagg aaaccatttg ccaatgatca caaatatgcg cgctggaaca cccgtcaagc
     5641 tcgaaggcag ttcaagaaaa ttgactccgc catacgtccc gcccgaggcg gatggagagt
     5701 tgtgccagtt gatgcgaaaa agattgcgag gatgaatatc ccaaagcccg atatccttca
     5761 attcattctt tacaccggac ggaatttttg atggatcttt catttgggaa taggtgggga
     5821 taatgatgtt tttctcacgg gcgcgtcgga tagcacgagc gcggcgatct ttgtgaatag
     5881 tcaggtcgat ggtggtcatg cgattctcct ggaaaattgt ctaacatatt ttacaatcat
     5941 ttcactcaca gtacgccctg gctttggcaa aattatcttc gaaggaatca accatatcca
     6001 atgtcagacg ttcttcatcc cagattgcat aacgttgttt tacgttttga acatcggacc
     6061 aggtcaattc gtgccagccg gaattctgcg ctcacgaagc tgcaatcgcg agcgatgaag
     6121 agattcgtct gtacatatac attcgatgac acgccagccg gctttatatt gatctgccaa
     6181 agcacgccag gccttccgaa cggtttccgt gctggctaca ctatccaaaa taaccgattg
     6241 ttccagcttt aattgtcgtt gggccagggt agtcaataga tcataacctg caaatcccag
     6301 tggtttttcg gcgattgatg gcgtcagttc acttcgtaat aggctcgctt ccagccaatc
     6361 cttagcaaat actggaatgc ctaatgtctt tgccaacgac tctgccagcg tacttttgcc
     6421 ggtaccgggc aaacccgaga atacgatcaa tttcatcatt acctcaatgc accaaattga
     6481 attttttaaa ggaagcgtat atatttttta atgtgtctcc aatgctgaag aagtatgata
     6541 acaacagaac gaaatagaaa ggaggtagcc atagaggttc cgcctgactt ttgtatagag
     6601 ggaaggactg ggtagtccga acctggtcaa agccactggc ttttgcacca tacgttctcc
     6661 gaacgaaaag gaagtgacat cccccacttc ttcctgaaac aatacatgtc tgtaaacctg
     6721 acggatgggt aattcccatc cgtcctttat ttaagcgcct tttttattct tttgaatccc
     6781 tcttccaacc atttacgcga cgtcccaaaa ttgatgcgga cgaattgttc gcttcctttt
     6841 ccgaattttc ggccgtcgct caatgccact ttcgcctcct ttagaaaaaa ccggaaagga
     6901 gagggcttaa gtttgaatgc ggaaaaatca agccaggcta gatacgtgga atctggaact
     6961 gtgatatgaa cctctggcag gtgccgtgta actgcatcca caagccaatc gcggttagca
     7021 gtgagatagc gacgcagact tcgcagccag gagtcacatt gaccctcata ggccacgcgc
     7081 gcggcaacca atccggcgct ggaaatatgg atgcccatat tgaatacagt ttctttgtac
     7141 gcctcgcgca gctgtttgtt gggaatgatc gcaaacgcgc agaataaacc aggaacattg
     7201 aatgtctttg aggctgagat taatgtaatc gtgcgatttg ccatttcacg cgcgagactg
     7261 gccatcggct ggaaagtggc atttccaagc aatagctcgc tgtgaatttc atccgagacg
     7321 ataatcaggt cgttcttaat acagatttct gcgatttgtt tcaactcttg aggcgaataa
     7381 atatgcccaa tcgggttatg cggatggcaa aggaggaata gatcggcctt cctggctgcc
     7441 tttccaaagg catcaaagtc gacctcgtac ctgatcctgt ttccttctac gatttttacc
     7501 agcggagatt caatctccct tacaccggtc ttcttttccg tatcgtggaa ctcgttatag
     7561 acaggcgttt ggattaagta ttgtttgcgt gaccggcaga aagttcttgc tgccacgttg
     7621 tatccaatat tcacgcccgt caaagtgaca atcatttcct ccgagatttt ccagcgatat
     7681 aaacgatgca ttcgttcagc gacggcttcc agtaattgtt tagatgggga ttcatagccc
     7741 atcacaccaa gatctaaata atgatgtaaa gcatctctga tgggtttggg tgactggaaa
     7801 tccatatcgg caacccacat cggtagaaca tcattgggat accaggtcca tttgtttcgc
     7861 agttgtgtag tgcgtcggtt gggaacgtcg tcgaagttgt agggcataga ggtcttgtta
     7921 ttctttgttg gtcgagcggt cccgcgagat cgtggcgaga ctgctcgacc ggcaaggtgt
     7981 aaatttattt cttatacgct acgatcatgc catcgcgcat gggtgtaaga gaaacaatcc
     8041 aatcggggtc ggtggtaatg ttctgagtga aacgtcggat cgcttctgtg gattgttctt
     8101 gatttttggg gtcaaagggg cgtccgctcc acaacatgtt gtcaatgatc aagattccac
     8161 cgtttcgcag ctttgctttg ataactggaa gcgaatcagg gtagccttct ttatcgatat
     8221 cattgaagat aatgtcaaac ggaccatcgg tttgacggag cgtctccacg gcttcggcgt
     8281 tgtgatattt gacgacttcc gagcgtccca atcgggacaa atgtccctgc gcgcgttttg
     8341 acagatcttc atcccagacg gtgtgatgaa cgaccccccc gccgttttca atcacggcct
     8401 tggcaaacca ggctgtggaa tagccatatc cagaccctaa ctcgaacacc gacgttgcgt
     8461 tcaacatgcg cgccagttga tagaggtaat atccacacga ggcaccgatg atcggaaagc
     8521 cattcttttc ggcataagcc tccatctcct gtagctcggg ctcacgggct gggacgaggg
     8581 aggtaatata atcctgcact ttgtcatatg aaatctctgc catttaattc tccttattaa
     8641 tagtttattg ttcgatgatt cttaaggcgt caggtaaggc taattttgcc cattctgcat
     8701 acatttttcc cgacggatgc agcccatccg aggcgatcag cgaaacatca gtagcggctt
     8761 cccgcgaaat tggagttaca tccacgtaat gaacccctgc ctttttcgat tcttcttcat
     8821 tcaccgcatt gaactgattg atttcgagcg caatttgttt actatcacga tcctttgcaa
     8881 aaggcgtgac gccccaatct ggaattgaga ggacgatgac ccgaccaggg tccccacccg
     8941 cgtatgcaat ggctttgttg agaaggaaaa caaatcctgc acgatactcc tcgatatctc
     9001 gtccacgata ttggttattt accccaatga gcagtgaaac cagatcataa ggcggctcta
     9061 ttgttacttc ctgaattccg ttccaaagtt catcagtcgt ccaacctgtg cgagcaataa
     9121 tggtcacttc tatacttccc ccggtctcgt cgaagggagg cgacgatcgt atcagttcag
     9181 caagttgatt cggccatcgc tcagcttcgg gcacgctttc cccgatcgta tatgaatctc
     9241 ccaacgctaa aaaacgaaca tcgggcatga catcatctcc aataatggtc ttggaagcat
     9301 tttgcgtgca ggcagtgagt agtaatagcc agcataaaaa aaaggatctc attcagatta
     9361 tagtcacaat tctcaactta tagtatttcg ctacataatt cacttaatgg gaattgaatt
     9421 ataaataaga atcaacagag gcacgacgat cattgccgtg cctctgttga ttggaatatc
     9481 ctatattttt gatttagctg gctgaggacg agtcaaacta aagattacca aacccagcat
     9541 cacgatggct gcaatccaac tgctcagcac aaccagaggt gcagatacct gcttgactgc
     9601 aatcccgaca aaggcccaga cgagcacaac caggtaagct gcatcacgtc gtgtcagtgc
     9661 cattgccagg ccaagcaggc tcgctactgc gagcatgatg actgcccagg tttgggctga
     9721 gacgccaaaa ccgttccatt cgacgaaata cagccaatcc gctatattag cgaccgttgc
     9781 cactgtaatc cagcccaggt aaagaccgaa tgggacatcc accgaccaat actcaacccg
     9841 cgtaactgat attcggttca cgttaagttg aaggtaactt gcaatcaaca tccccagcaa
     9901 tataagcatt atgagtacgc ttaaaccaaa gcggttgtaa tgccagcaga aaagccaccc
     9961 tgcgttggca aggttgctaa gagcaaacaa ataacccagt cgccgcagac gcggactttc
    10021 tttttgtgac cgttgaaatt gaaaaattgt gaatgcgatc cacccgatgt aaattattcc
    10081 ccagatggca aagacgtaac ccgctggcac aaaatagacc tcgaaacgat ctgaaatttc
    10141 gccggtgttc tgtccattca atggcagggt ggacgcgagt atattaatcg tgagagcaag
    10201 aatgacgctg aacaaattag caaactgacg gattgtatcc atggacatgc actttatcct
    10261 tgtaaatatg atactaaatt gaatatagtt agaacctaaa cggtcagaac tgggagccgg
    10321 gtaaatagga ctatagcctt tggagcaggg cagggattcg cctcgcctta ccccagctgc
    10381 tgagggacgc gcgtcgggta aaaacatcgt aatcgttctt ttcgatctca tctagaattc
    10441 cctgatagaa aaccgatgcc gctccgatag caagctgacc ttcgcgctca agcattttta
    10501 ccccttccca ggatgcatca tagagctgac gtgttcggtc aatttgaaac ttcataaact
    10561 gacgccaatg gtttgttaca cggcctgctg caatatcttg ttcgttgact ccataaaaag
    10621 ccatttcttc acgtggtaga taaagacgac cgatctgatg atcctctccc acatcacgca
    10681 aaatattggt catttgtaat gcaaccccga gcttgatggc ataaggaacg gcctcattac
    10741 tatggaaccc gatgatgtac atactcatca atccaactgt ggaagcaaca ccatagcagt
    10801 aggtggtgag ttcttcaaag gtttgataac gtgactgagt cagatcgcgt gcaacaccgt
    10861 ctatcagttg tagggcatag tggcggggaa tatggtatcg gatgatagtg tcggcccacg
    10921 cggcggcgac caggtcgtcc tctgagaagg aggctgtttc caccatttca cgccagtaat
    10981 tcaatttttc ctcgcgttca atatctgttg actcatcaac aatatcgtcc acgactcggc
    11041 agaaggcata tagagcgcgg accgcggaac gcttttcctc agggagaagt gctgacgcaa
    11101 aataaaaaga tttgctgtgt tcagaagtga tttgctctgc ctgtttataa gccgaactca
    11161 gggcagcatc acctgcccaa taagaaaaaa ggggccgcgt agaggggtgg ggggtattcc
    11221 cggcaagagc gagaagttgg ttttcccagt tagcgtgtac ctgcatggta aacctccaga
    11281 aggtaaagat agaggtattg tattgatttc ggtctgtttg tcaaggtttt gcaaaggaaa
    11341 atttacaatt taccttgaca aataatggac aagatgtata atgagattta aatctttcaa
    11401 ttcctcctcc actgggggag atatctggag caaaatgaac aaaccaaccg caattgtgat
    11461 cggcgccggc atcggtggaa tcgccacggc tgcccgcctg gctaaaaatg gatatgatgt
    11521 aactgttttg gagaaaaacg agctaccagg tggacgctgt aatcagattg tccacaatgg
    11581 tcatcgcttt gatatcggtc ccaccctctt tctgatgccc gaggtttggg aagagacgtt
    11641 tgcctcgttg ggtgaaaaaa tgtctgacca tctggacttg cgccggatag accccaccta
    11701 taaagttcac tttgatgatg ggctcaaact ggagttgact tcaaatatcg gcacgatgca
    11761 aactcaactt gaaaaggttg aaaagacggc attttcgggc ttcctgaact accttgccga
    11821 aggaaacaaa cactacaaaa tttcgcttga gaaattcgtc ggaagaaact tctacactat
    11881 ttttgaatat ttgagcctgg cgaacctgcc gttgatgttt aaactgaaag cactggcaaa
    11941 acaccatgcc aacacaggtc ggtatttcaa ggacgaaaga ctcaaagccg cctttacgtt
    12001 tcaaaatatg tatctgggct taagccccta cgatgcgccc gctacttatt ccctgctcca
    12061 atacactgag cttgctgaag gagtgtggta tcccattgga gggatgtatg ctgctattca
    12121 ggcactggtc aggattgcag aaaaacttgg tgtcaggttt tcctacaatg ctcccgtgag
    12181 gaaactcgtg accaataaaa acaaagtgct gggcgtggag ttggaaaatg gacagtctct
    12241 ggttgctgat atagtcattg gaaatgcgga cctcccctat atttacaaaa accttttgcc
    12301 tgataaagct gaagcaaaga aactggagaa aaagctttat acgtgctcca caatcatgtt
    12361 ttactgggga gtggataaag aatatccgca gattgcacac cacaatgtgt ttttaggtgg
    12421 ggattacaag gcatcgtttg atcagatctt cgagaatcac accctgcccg acgtgccttc
    12481 cttctatgtt catgccccag ctcgcacaga cgagaaagct gcaccccaag gtcaggatac
    12541 gctgtacgct ctcatcccag taggccatct ggatccacac caaaaacagg attgggacgc
    12601 gcttgtgaac cgggcacggg aaacagtctt cgatcgtctg gcgaaagaac tgggcgtgac
    12661 tgacctgaaa gaacatatca agtttgagat tgtgtacgat ccagaagtct ggcaggaccg
    12721 cttcaatctc gctcgaggcg cggcatttgg actcagccat aacttctggc aggtgggata
    12781 tctccgccct caaaaccgtc ataagaagta taagaatctt tacttcactg gcgcgtccac
    12841 tcacccgggc actggtctac cgatcgtcct gctttcggcc cgtctgacaa ctgaacggat
    12901 tttgaaagaa atgagtacaa ttgctgtgca aaaggcggtc aaccccgtcc taaatactgc
    12961 acaaggaaac taaatggtca gcacaactcc tgctttcaat ctgaaagttg tcctcaaaga
    13021 gactgggctt gcggcagaca cgttgcgtgc ctgggagcgc cggtatgggc ttcctgtacc
    13081 gcagcgcact cgtggtgggc atcgtcttta ttcgcaacgt gatatcgaga ccatcaaatg
    13141 gctgatgaag cgacagacgg aaggcctttc gatttcacgc gctgtggata tgtggaatga
    13201 gcagctcgcc gcaggttcag atccgctggc cgatcttatc gattcgttgt tagcctcagc
    13261 atcccctgtt ctctcacaga atcgcactcc agacacgact cttgacttgc ttcgatctca
    13321 atgggtggag gcttgcattg agtttaatga gtcgaaggct gagcaaactt taaatcaggc
    13381 attttccatg ttcccggttg aagttgtctg tttagacgtt ttacagaggg gcttatctga
    13441 aattggtgat ctctggcagg aaaaccaggt cagtgtgcaa caggaacact ttgcctctgg
    13501 ccttgccatg cgccggctgg atgcgttatt gatctccact ccgcctccaa cacggaatca
    13561 aattctgctg atcggctgcc cgcctggcga gtggcatgtt ttcaccccgt tgctgtttgc
    13621 tctctttctt cgccgccgcg gattcccctt gatatacctc ggcggaaatg tacccgcgcc
    13681 gcgctttgcg gatacagtta agaacgtaaa agcagacctg gttattctca tcgcacagca
    13741 gctcgtcact gcagcgaccc ttcaggaaac tgtattccta ttagcgaagc aaaaaatctc
    13801 ggtggcattt ggaggtcgga tcttcaacgt gcaaccggag atcacgaatt ccatttccgg
    13861 ttatttcctg gggaccgaac taattgcagc attggatgaa attgaattga ttctgagaag
    13921 caaagcgact caccgacagc aaaaaacggc ttctcaggaa tacataaccg cccatcagtc
    13981 cttcacatcc aagcggaccc agattgagct gacactcaag gaaacactgg aaccgaattc
    14041 catttcaccg gaagatgtca aaacgggaat acacttcctc ggagacaata tcacggcggc
    14101 tttgcaatta ggtgatatga gctacgtctc ggcggaggtg gattggttga aaaatttgct
    14161 ccaggcgtac gaaacgccat cacagcaatt gatccatttt atgaaaacgt atgccagatc
    14221 cctggatcaa gccatggacg gacagggtaa acctatcttt gaatggttcg ccgccgaaat
    14281 ccaaaaattg gataacgatg gcttagtgga ggattattga tgcaagcagc aaagactaat
    14341 ccgttattgc gttaccttct tccgggaatt ctggtttcgg cggcatttac cgtcattatt
    14401 gggctgacag ctgcaaactt gacgggattt caaattgttg gcgatacgga tatcctgttc
    14461 aactacccct ggcagttgag ggagccaacc gcgctggcgc gtctcactgc ctggaccgga
    14521 tacttgattc acaatctgat tgtatgggca atcatttact tcgctcaacg agaaaaacca
    14581 aagttcggta atgacttgcg ctggttcaat tgggcgatga tttggacaca tgtcactttt
    14641 attgttttgc atatcgtcca aactcagttg ttctacgatg gattggcaca ggatgttccc
    14701 gaagtcactg cacaggggtc ggttgcgttg atgctgatca tcatcatcct cttcgagacc
    14761 ccgcgacgcg gattgatctt cggaaagaag ttcaaattca aagatggctt catgtatatt
    14821 gcccgcaaat atcatggcta tttctttgcc tgggctacca catatacatt ctggtatcac
    14881 cccacagaag ggacgtgggg acacttaatg ggcttccttt atatgttcat gctcttcgtt
    14941 cagagcgcgc tgatcttcaa ccgcgtgcac ctcaacaagt ggtggacgtt tactctggaa
    15001 gttttcgtcg tcattcatgg cacagtcgtg gccgtcttac agggaaatgg aatctggcca
    15061 atgttcgcgt ttggcttcgg tgcgatgatc gtgctcacac agatgtacgg actgggtctc
    15121 aacacatgga cgaagcgcgc gctggcgatt ggctttgtcc tcatgatcgt agcctattac
    15181 acactcttcg atagcattgc aggtctaaat gaagtgattc gtattcctgt gattgattac
    15241 gttgttatct tcctgctgta tggaatttat cttttcatca attggcttgt caacattttc
    15301 aaacggccaa caccaatgca ggcagggaca gattagcgat ggccgggtcg ttgaaagaca
    15361 aaatcgttgt gctcacgggc tcaacacgcg gctttgggta tgccatcgca gaagcaatgt
    15421 tgaaggctgg cgccactgtc attgtcagcg gtcgttctga agaagtgctt gcaaaagcaa
    15481 ctgcttcact gcggagtcac ggtgacgtca aaggtcagat ttgtgacgtg agagaaaagt
    15541 tacaagtcta tgcattggcg cggtttgcta ttcaatcttt tggtcgaata gatgtatgga
    15601 tcaacaatgt aggatactcc tccgctgccg gcgagatgct ggaaacaccc cctgaacagg
    15661 caatcgatat gttcctcgcc aatgatatgg gaacactcta cggttcgcaa gccgctctgc
    15721 attatatgtc gcagcaaaga caaggcacat tggtcaacgt gtatggaaat ggttccttcc
    15781 tgcgacctgc ctctccgacc ggcctgtacg gcgcaaccaa agcctgggta acttccttca
    15841 cgcgatcact tgccaaagag atcaagggcg gtggcgtcaa cttgattggt ttcagccccg
    15901 gcatgatgat gacagatatg ctgaccaatc cggttgtgat cggtgagcgc ggcaaggaaa
    15961 tgatgaaacg atattcattt gtattgagat tcctggggga tgacccaacc aaagcggccc
    16021 agcaactcgt cgaaacaatt gccaataata aaaaggaatt tattgaatat caaatgctcc
    16081 gcccatggac gatcactttc cggcttttac gcgtgatgtg ggaaaatctc accaaaacag
    16141 gtcagacgcc ccaatacacg ttgcattacg aagaagcata caagcctgat ataaaatgaa
    16201 caataaatat ttattcggta tcatcctcgt tgtcttcatc gacctgcttg gttttagtct
    16261 gatcattccc ttgttgcctt attatgccca gacgtttaac gccagcgata ccacaatcgg
    16321 attgttgctg gcttcctacg ctgccgctca attgatcggc gcgccgttac tcggaagggc
    16381 ttcggaccaa tttggacgac ggcccatttt gctggtgagt attttcggaa cgtttttagg
    16441 gttcctgctt tttgggcttg ccaattccct gacgatgtta ttcgccagtc ggattctaca
    16501 aggaattaca ggcggtaacc tctccgtggc acaggcgtac atcacagatg tgaccgacgc
    16561 gcagtcgcga aaccgtgggc tagggatgat cggtgcagca tttggcctgg gcttcatcat
    16621 cggtccggca ctgggcggtg tccttagcaa tatcagttac agcgtgcccg cttttgtggc
    16681 ggccgggctg tcattcatta atttgatact gatcattttc tggctgcctg aatcgctcac
    16741 gcctgaacgg cgcgcacagc tttcgccgaa aagatcgggc atcacactgc aagcgctttt
    16801 agagtctctc cggcgtccgc tgacgggcac cctgcttatt actcggtttt tatatagcct
    16861 ggcgtttgtg atcctgcaaa gcatcttttc gttgtttgcc ctcaggcgat ttggtatgag
    16921 cgttgttgcc acaggttttg tacttacata tgtgggtgtt gtttccgttg tcacgcaagc
    16981 ctggctaatc ggcaaactca gtcagcggtt tgaagacatg gtcttgatcc agtttggatt
    17041 agttcttttg gggatcggtt tactgacctg ggccttcgcg ccgaatatcc cgtttcttgt
    17101 tttgtccgtc acgccgattg cattggctgg tggcatgctc aataccattc tcccttcttc
    17161 cctcacgaaa acggtcgagc cgcaggatgt tggcggaatt ctggggctat ctacatcgat
    17221 tgaaagctca acgcgtgttg ttgcaccatt gctaggcgga tttatgctcg acaatatctc
    17281 ttactgggcg cctgggctgt tcggcac
//
LOCUS       DASTJP010000116         6931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_980852, whole genome shotgun sequence.
ACCESSION   DASTJP010000116 DASTJP010000000
VERSION     DASTJP010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980852"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1234)
                     /locus_tag="VFQ23_03665"
     CDS             complement(212..1234)
                     /locus_tag="VFQ23_03665"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9905704.1"
                     /translation="MNQAQLVESLDTSIQNHAFSGAVSIRQQGQVLYERAAGYADRSN
                     KIPNTLDTRFGIASGTKFFTALAVGKLIAAQKLDFTTKLSDCLGLDFPQYSPEITIQH
                     LLTHTSGIPDYFDEDKFTDFDQFFVSRPWYELKGPRDYLAVFPDEPMKFEPGTNFSYS
                     NGGYILLGIVIEELTGMKYQEYVTQEIFRATGMDRSGYFAFNQLPEKTAWGYIEAEGG
                     WRTNIYNLPIVGASDGGAYTTVDDLAILWKAFWGNEILPRELVEVYATPYVKGEGEDT
                     YYGHGLWLEKGTSAMPEVYFMGGDAGVSFKSSVNRDEDLLVTVISNTTNGAWHMLRDI
                     KSALKE"
     gene            complement(1356..1874)
                     /locus_tag="VFQ23_03670"
     CDS             complement(1356..1874)
                     /locus_tag="VFQ23_03670"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905705.1"
                     /translation="MDDIQIELIQPGQVDEVIELFTHHVYEGNRDEAVTHLAGHREGE
                     ADTFVAYVGRNLAGFLTVRWHSNYPGFRDQNIPLIHQIEVFTPFHRHGVASQLMERAE
                     RLIATRSRQAGICVGLFDAYGPAQRLYAKRGYVPDGQGACQDHRPLKYGETVTITHDL
                     LIWMTKELPSPL"
     gene            complement(2082..2993)
                     /locus_tag="VFQ23_03675"
     CDS             complement(2082..2993)
                     /locus_tag="VFQ23_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9905706.1"
                     /translation="MDNEYSKLSRASTLKELEAVLDKFFDETKTQLGLAFKPQPSDII
                     ITPYGKCGTTLLQQIAHGLRTKGSLDFDEINSVVPWIEIAYDVGWDLDAPQLAEPRLY
                     KSHLSWHNIPKGCRYICSFRHPYTASVSFYRFFEGWMFEPGTISLEDLTRWRWSRDKA
                     DRQGYWYHLTSWWEQRHNQDVLLLCYEDMIVNPSHAIQRVARFMGIDLDKSLLDIIVR
                     QSSREFMLAHSEKFNEHHIRDIGGKRAGLPPPISSSKVTSGVSNDARYTLPLHLKQEL
                     DEIWQEQVKPKFGFENYEELRQALQNL"
     gene            3309..3677
                     /locus_tag="VFQ23_03680"
     CDS             3309..3677
                     /locus_tag="VFQ23_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905707.1"
                     /translation="MGTIPALSPDETTPLERNQLQATKSACLHLAAGTGENAHAVPTG
                     GSPTTPAPAYSPAPPTGAGVAGVPVHVEDCRKATCVAMGAVLAAVCIGRIFKVVSREW
                     ISHRELSWVAYPSVGCHLMV"
     gene            complement(3855..4391)
                     /locus_tag="VFQ23_03685"
     CDS             complement(3855..4391)
                     /locus_tag="VFQ23_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905708.1"
                     /translation="MEKKPLDQQVVRVMAWLLLYGRAKSKLGAEQWEALEKSVGAPPF
                     KQMDPNDITPLTTFNAALDFIGNSRDQADPSLIAEAADDSVLGWAQQNRAKVQELQGK
                     PRELLDLWLTQVHPWFFNETENIEFIESGPNHMLVKLYHDMRDEFTMGLFTGLIKLVG
                     AKGQIENRGDQLYYVTWQ"
     gene            complement(4707..5885)
                     /locus_tag="VFQ23_03690"
     CDS             complement(4707..5885)
                     /locus_tag="VFQ23_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9905709.1"
                     /translation="MKTKIALRKQAVALYLQGRKKSEIARMLQRSRPWVNRWIERYRA
                     EAPRLSLRDRSKAPKQRRCAYPDRIKRMVLQIREEREQGQRSKYQYALVSAQAIYYEL
                     RELGVSPLPSSRTIHRWLKRAGRIPERRSQKAPNLTYPVLPCQAVNDVQELDFKGPFY
                     LQDHSHKYYLVALRDKWSKKTALRALATKSMDAILDFLVSAWQKLGCPRYLKMDNCLD
                     FRGSNRHPRAPSKLMRVCLDLGVQPVFIPICEPWRNGVVENLNGLLDRFLFRTSTFET
                     EKQLYKAVQQMETTINTTHHLSTLQGKTPQEFAAHAKLCYPPTKYDWRTRDLRLLKGK
                     VTFLRFVRKSGRITVTARDKFLIGKKYKWHYVMAVVDVARKRLNVICQGKHIRSFDYR
                     "
     gene            complement(6204..>6931)
                     /locus_tag="VFQ23_03695"
     CDS             complement(6204..>6931)
                     /locus_tag="VFQ23_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905710.1"
                     /translation="YSLAQQSGHWTLGNLRVFQAFLYASAYFWRPAVRPITTNASRWA
                     VPLQIVNQASFLILRNLLYPPFVIASKEKTMKRKLSLALWVAILMINGCARLATATPI
                     PTDTSAPTDVPATTIVLPPTTTPTPIISREQVIEPKLGDEFWLKMGESVTLQKGGLTI
                     QFKSLAGDSRCPEGVLCVWMGNAEVILEVSKNEIRLNTLLDPKEEVVGDYNIQLRDVA
                     PYPKAGVEHTPENYSIKIVVNKK"
BASE COUNT         1680 a         1749 c         1713 g         1789 t
ORIGIN      
        1 tcaaagccca tcaattcatg atagaagcga atcgatcgct ccagatcagc tacttttaga
       61 tgcacgtgac caatggaagt tccagcagga atcgaatcag acatgttaaa tgatctcctt
      121 tatgtgagag gcaaggttaa tatgtagatg cttcacaaac gatgcttttt acggctcccc
      181 actcattcgt ttctcgcgat tccaagccag gctattcctt caatgcgctc ttaatatctc
      241 gcaacatatg ccaagcacca ttcgttgtgt tagaaatcac tgtgactaga aggtcttcat
      301 cccggttcac gctggacttg aaagagaccc cggcatcgcc gcccatgaaa tagacttcag
      361 gcattgcact tgtacccttt tcgagccaaa gcccgtgacc gtaataggtg tcttcacctt
      421 cgcctttgac atagggcgtg gcgtagactt cgacaagctc tctcggcaga atctcattcc
      481 cccaaaatgc cttccacaag attgccaggt catctacagt cgtataggcc ccgccatcgg
      541 atgccccgac aatcggaaga ttgtagatat ttgtccgcca acctccctct gcttcgatat
      601 agccccaggc tgtcttttcg gggagctggt tgaacgcgaa atacccagac cggtccatcc
      661 cagtcgccct aaagatttcc tgagtgacat actcctgata tttcatgccg gtcagttctt
      721 caatcactat acctaaaaga atatacccac cgttcgaata cgagaagttt gttcccggtt
      781 caaatttcat cggctcatcc gggaagacag ccaggtaatc tctgggaccc ttcaattcgt
      841 accaggggcg gctgacgaaa aactggtcaa aatcggtaaa cttatcttcg tcgaaataat
      901 cagggatgcc ggaggtatgt gtaagcagat gctgaatggt aatttccggc gaatactgcg
      961 ggaagtccag cccgagacaa tcactgagtt tggtggtgaa gtcgagtttc tgcgccgcga
     1021 tcagctttcc gaccgccagg gcggtgaaga attttgtccc ggatgcaatg ccgaaccgtg
     1081 tatctaatgt atttggaatc ttgttgctcc ggtcggcata gccagcggct cgttcgtata
     1141 acacctgtcc ctgttggcga atcgatacag cgccagaaaa agcatgattc tgaatcgatg
     1201 tgtctaatga ttcgaccagt tgcgcttgat tcatttctta tcggacctct ctcgttttac
     1261 tatcttcgat ggttgacgtt caaactagtt gttgcacctc gtagattgtc gcaaagggga
     1321 ggtgcccgat gatgagagct gtttttgaga gttcactaca aaggtgaagg aagctcttta
     1381 gtcatccaga tcagcaggtc atgagtgatg gtgacggtct ctccgtattt tagcgggcga
     1441 tggtcttgac aggcaccctg gccgtccggc acatagcccc gtttcgcata caagcgttga
     1501 gccggaccat aagcatcgaa gagacccaca caaatgccag cctggcggga gcgcgtcgca
     1561 atcaatcgct cagcacgctc catcagttgc gaggcaacgc cgtgacggtg aaatggcgta
     1621 aatacttcta tctgatgaat gagggggata ttctggtctc gaaaccccgg atagtttgaa
     1681 tgccagcgga cggtcaaaaa gcccgccaga tttctgccga cataggcaac aaacgtatca
     1741 gcttcacctt cgcgatgacc agccagatgc gtgaccgcct cgtcgcggtt cccctcatag
     1801 acgtggtgtg tgaataactc aatcacctca tctacctgcc cgggttgtat gagttcgatt
     1861 tggatgtcgt ccatctgtcg atgtcctttg atgattatta tagccgccca atggttggca
     1921 ttacccgctt aaagcggaac agtggggtcg ccctttgtcg gaatcaacgc taagcttcct
     1981 acgtgcttga aaatgcccag atttggcggg cgagctttag acgaacgcac atgtcccagg
     2041 accggagcga attggatcgc ccagcgaaag cgcatttttt attacaaatt ctgcagcgcc
     2101 tgacgaagtt cttcataatt ctcgaaccca aattttggct taacttgttc ctgccagatt
     2161 tcatccaatt cttgcttaag atgcaatggc aatgtatagc gggcgtcgtt cgatacgcca
     2221 gaagtgactt tgctggaaga aatgggagga ggtagcccgg cgcgttttcc gccaatgtcc
     2281 cgaatatggt gttcattgaa tttttcacta tgtgccaaca taaattcacg agaagattgt
     2341 cgcacaataa tgtccagcag ggacttatct aggtcgatac ccataaaccg agcgaccctt
     2401 tgtatcgcat gggatggatt aacaatcata tcctcataac acagcagtaa aacatcctga
     2461 ttatggcgtt gctcccacca tgaagtcaaa tgataccaat agccttgcct gtctgcttta
     2521 tcacgagacc aacgccagcg agtcaggtct tccaaactga tggttccggg ctcgaacatc
     2581 cagccttcaa agaaacgata aaaagataca ctagcagtat aagggtgtcg aaaagagcag
     2641 atataccgac atcccttggg gatgttgtgc cagctcaaat ggcttttgta gagacgaggt
     2701 tcagctaact gcggagcgtc taaatcccag cctacatcgt aggcgatttc gatccatgga
     2761 acgacggagt tgatttcgtc gaaatccagg cttccttttg tgcgcagtcc atgagcgatt
     2821 tgttgaagca gggtagtgcc acacttacca tagggggtga taataatatc gctaggctga
     2881 ggtttaaagg ccaagccaag ctgcgttttc gtttcgtcaa agaatttgtc gaggacggct
     2941 tctaattctt ttagagtgct cgcacgggat aatttgctgt attcattatc cataacaact
     3001 ccttaattag acagcgtgga caatgttacg cttaattcgg gcaagtgaat catgacttgc
     3061 cttccgtatc gggagcaaga gaaaaacgaa gaaaaaattc ctgcataagc tcctgagtag
     3121 ctgaaacttc ttttgcgtct tttgtagatc aattgaccac ataactcaca tccaagcttg
     3181 tgtatccatg aatctaacac aaaattattt taaatgtttt attgtggcat tgagccccag
     3241 tgctgactgg ctagcgtttg cgtagcaact gtaaaagcta tgccccttca gggtactggt
     3301 gtgcgagatt gggcaccatc ccggcgctct ccccggacga gacaacgcca cttgagcgga
     3361 accaacttca agctacgaaa agtgcctgcc tgcaccttgc cgcaggcaca ggtgaaaacg
     3421 cgcacgccgt ccctacgggc ggcagtccca ccacgcctgc cccggcgtac tccccggcac
     3481 cgcccaccgg ggcaggtgtt gccggggttc cggtgcacgt cgaagactgt cgcaaagcga
     3541 cgtgcgtagc gatgggggct gtgttagccg cagtttgtat tggtcgcatc ttcaaggtcg
     3601 tctcgcgaga gtggatttct catcgagagc taagttgggt tgcttatcca agtgttggct
     3661 gccatttgat ggtttaacgg tttaaaaatt accaaaatga tttccccaag taagtcaatt
     3721 gcctgcaaat gattgatttt atgaattata ttccggctgc cgcccactga aggacaaggc
     3781 gtagcatctg agcacggcca tttggcgtcc ttctctgcag gtggtgttgg gcagccggaa
     3841 ctgtaacagg cgagttactg ccaggtcaca tagtagagtt gatccccccg attttcaatt
     3901 tgccctttcg caccaacgag tttgattaaa ccggtaaaca atcccatagt gaattcatca
     3961 cgcatatcgt gatacagctt caccaacatg tgattcggcc cagactcgat gaattcaata
     4021 ttttcggtct cattgaaaaa ccacgggtgg acttgcgtca gccatagatc tagcagctcg
     4081 cgcggtttac cctgcaactc ttgcactttc gcacgatttt gctgagccca tcccagcaca
     4141 gagtcatcgg cggcttcagc aataaggctt ggatcagcct gatcacggct attgccaatg
     4201 aagtcgagcg ccgcattgaa ggtggttagt ggggtgatgt cattcgggtc catctgtttg
     4261 aacggcggtg cgcctacact tttttcgagt gcttcccact gctctgcccc gagtttggat
     4321 ttggcgcggc catataaaag aagccaagcc atgacgcgga ctacttgttg atctagcggt
     4381 ttcttttcca tcttattatc ctttgtccgg agttggtgaa ggatacggac ctgctgacca
     4441 acgccaaacg ctattgcgct ggcgacctgc tcccctcaat tagattttag gcattaggct
     4501 gcaaacgatt gaatgcatgg attatactct actatatccc caagtgattt taccgatttg
     4561 gtgactaaat gaattaaggc tgatggcaaa cagatatctc aaaagctatc taatgcggct
     4621 aacggctcgt gccaagtgac acgacatatg ttacacctgt ttctaacgaa gagaactgac
     4681 ataacatccg ttacactttt tatcattcag cggtaatcaa aggaacggat atgtttgccc
     4741 tggcagatca cgttcagtcg ttttcgggca acatccacaa cagccataac gtagtgccac
     4801 ttgtattttt tgccaatgag gaacttgtcc ctggcggtca cggtaatcct gccgctcttg
     4861 cgaacgaaac gaaggaaggt caccttgccc tttaacaacc gaagatcacg cgtgcgccaa
     4921 tcgtacttgg tgggtggata gcaaagcttg gcatgagctg caaactcctg gggagttttg
     4981 ccctgcaggg tggacaagtg gtgggtggta ttaatggtgg tctccatttg ctgcaccgct
     5041 ttgtagagct gcttctctgt ttcaaaggtg cttgtgcgaa acagaaaacg atccagcaga
     5101 ccgttcaggt tttccaccac cccgttgcgc cagggttcac agatcgggat gaagacaggc
     5161 tgtacgccca gatccaggca tacccgcatg agcttgctcg gggcacgggg gtggcggttg
     5221 ctgcctcgaa agtccaggca attgtccatc ttcaagtatc ttggacagcc cagcttctgc
     5281 caggcagaga ccaaaaagtc caaaatggcg tccatactct tggtggccag ggctcgcaga
     5341 gcagtctttt tgctccattt gtctcgcagc gccaccagat agtacttgtg cgagtggtct
     5401 tgcaaataga acggaccctt gaaatccagt tcctgcacat cgttcaccgc ctggcagggc
     5461 agcaccgggt aggtgaggtt cggtgctttc tgcgatctgc gttccgggat ccgccctgcc
     5521 cgcttcaacc agcgatggat cgtgcgcgag gagggcagcg gagaaacgcc caattcccga
     5581 agctcataat agatcgcctg tgcactcacc aaggcatatt gatatttgct tcgctgtccc
     5641 tgttctcgtt cctcacggat ctggagcacc atgcgtttaa tccggtctgg ataggcgcac
     5701 cgcctctgct ttggggcttt ggaacgatcg cgcagactca accggggtgc ttctgcacgg
     5761 taccgttcga tccaccgatt cacccacgga cgcgatcgct gcagcatcct ggcgatctca
     5821 ctcttcttcc ggccttgcag atacagggca acggcttgct tgcgcagcgc aatcttggtc
     5881 ttcatccttc cacctcctgg ctccacaccg gaaggtgtaa cagatgttct gtcagttttt
     5941 ggcgttagaa ctgtaacgca tgtcctgtca gtttttttgt aacggatgtc atgtcagttg
     6001 gcagctcgcg ttagtggcgg ctgatgagga aaaggcttag aagcgggaaa ctctcaaagc
     6061 ccaggaaaac gccaaaaaac gcggcgcgta ccagccatcc ggtgcacgtc gaagactgtc
     6121 gcaaagcgac gtgcgtagcg atgggggctg tgttgggcgt ggttgaccat acgactcttt
     6181 agccttctgg ttagcagcgt gctttatttt ttgttgacta caattttgat actataattt
     6241 tccggcgtat gctctactcc agctttcgga tagggagcta catcccgcaa ctgaatatta
     6301 taatcaccta ctacttcttc ctttggatca agaagggtat tcaatctaat ttcatttttt
     6361 gacacttcaa gaattacttc ggcatttccc atccaaacac aaagcactcc ttcaggacat
     6421 cgagaatctc ccgccaaaga cttaaactgt attgtaagac ctcccttttg cagggtaaca
     6481 ctttcaccca tcttaagcca aaattcatca ccgagttttg gttcgatgac ctgttctctt
     6541 gaaataatgg gcgttggcgt ggtagtgggt ggaagcacga ttgttgttgc aggaacatca
     6601 gtaggcgcag acgtatcagt gggaataggg gtcgcagttg ccaatcgagc gcaaccatta
     6661 atcattaaga tggccaccca aagggcgagg cttaattttc ttttcattgt cttctcttta
     6721 gatgcaatga cgaatggcgg gtaaagaagg ttccgcaaga taagaaagct cgcttgattt
     6781 acaatttgta agggaaccgc ccaacggctc gcgttagtgg tgatggggcg gacggcggga
     6841 cgccagaaat aggctgaggc gtagagaaat gcctgaaaaa cgcgcagatt ccccagagtc
     6901 cagtgcccgc tttgttgggc gagactatat c
//
LOCUS       DASTJP010000117         6031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1008352, whole genome shotgun sequence.
ACCESSION   DASTJP010000117 DASTJP010000000
VERSION     DASTJP010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008352"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..467)
                     /locus_tag="VFQ23_03700"
     CDS             complement(117..467)
                     /locus_tag="VFQ23_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905711.1"
                     /translation="MAKKQDDSIGDNDYIEQLQWQASFRRRWPIRFTPKWKYKIIYNN
                     EKTGPGATIAAWAMLICVASIVIFTLNSFVRSGEIGKAIAFGVLFGMIGIILHFAARD
                     ISKEQEDDHENFEE"
     gene            complement(485..1291)
                     /locus_tag="VFQ23_03705"
     CDS             complement(485..1291)
                     /locus_tag="VFQ23_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET9905712.1"
                     /translation="MKNKLNDLTSKEWLKFQKSWFIHNPPPRKKGVLVHPAKFPETMA
                     QEFIEFFTKKGETVLDPMAGTGSTLVAALRAGRNSYGIELNPKYAKIATQIIEEERNL
                     LGDSIQNLKSEIVNGDAAQVANYQFPMIDYVLTSPPYWDMLRARGAQTQKKRRASDEL
                     DVHYSDDPNDMGNISDYNEFLEKLVDIYKGLRPLLREKAYLTIIVKNVKKGGKIYPLA
                     WDIARELGKTYALKDEKIWVQDNQRLSPFGLGSAWVSNTFHHYCLQFRNE"
     gene            complement(1409..2161)
                     /locus_tag="VFQ23_03710"
     CDS             complement(1409..2161)
                     /locus_tag="VFQ23_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905713.1"
                     /translation="MTLLTGACLPFDSTPPTATPLPIDTSTPTPTIVWFPPSATSTHL
                     TFPTYTATPEMKPGVESENLTDNFSDMKLWDTAVSDQGSAAISRNRLSLAIQPGVYLA
                     SMRREGTFGNFYAEITARPSLCRGTDNYGIIVRSVGSSYYRFVLSCNSLVRAERVNGG
                     IRLPLVDPLPSGDAPRGAPGEVRIGLWAVGSEMRLFLNDRYQFGIKDKSFPSGAIGVF
                     VRSESDTPMSVTFSDLKVYDVNYVPPTSTPLP"
     gene            2390..3412
                     /locus_tag="VFQ23_03715"
     CDS             2390..3412
                     /locus_tag="VFQ23_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9905714.1"
                     /translation="MTEQRAQRVLVTGACGEIGQALIQALARRGGYEIVTSDLSALPD
                     SIKALSFEHVQGDLVYKVKNFYDYDFDVIFHLAASLSSKAEVASEEAHRINVEGTMQL
                     LMLAAYRSEKYEKSVKFLFPSSIAAYGMPSLEAKTEAGSVTEQVYNTPQTMYGCNKLY
                     CEKLGMYYSKFYGQKHLDEEPPCMLDFRSIRFPGLISASTLPSGGTSDYGPEMLHAAA
                     QGKPYTCFVREDTKISFMAMPDAIKSLLMLLEVPREKLNNQVYNIAAFALTASEFRDR
                     AVRAFPGAQITFKPNPRRQGIVDSWPEDVDDSLARSEWGWKPDYDADAFFDDYFLPEI
                     RKRYGK"
     gene            3471..4313
                     /locus_tag="VFQ23_03720"
     CDS             3471..4313
                     /locus_tag="VFQ23_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET9905715.1"
                     /translation="MKIFTALVAFLFVFVLIFISTIPSPTVVNAAPNAQFVTATPGPD
                     GRILYTVVDGDNCSIVAFNHGITVPDLRALNTRLDENCTLTVGQQIVVGLAQPEGPTA
                     GPGPTLPPATVTATPVSGTTEVCVLLFDDMNGDALRQETEFGIEGGAVSLTNLNGSYS
                     ETQNTTAAIDPDLVEPIRSCFIDVPQGEYNVSIAVPDTYNPTMLLSYTLTVRAGDRAS
                     IDFGAQSKTVTVSETTDTQGGRSSMLGIFGLLLLLSGAGLGYYAYRSGQPRSKLKGSP
                     LTKR"
     gene            complement(4355..4702)
                     /locus_tag="VFQ23_03725"
     CDS             complement(4355..4702)
                     /locus_tag="VFQ23_03725"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET9905716.1"
                     /translation="MTSSCIFCKIVSGEAKSTIVFRDDQVTAFRDLNPVAPTHILIVP
                     NRHIESVSVLKLEDEPLMGHLFTVASQLANEEGISNGGYRLITNTGVDGGQTVLHLHL
                     HLIGGQRMRYPMG"
     gene            4985..>6031
                     /locus_tag="VFQ23_03730"
     CDS             4985..>6031
                     /locus_tag="VFQ23_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9905717.1"
                     /translation="MNETSLTLLIFILAFGGMVIIHEFGHYIVARLFKIEVEEFGVGL
                     PTPGALTLWINKGYLLLDDGGRIEIPYNFKLPVNWNQLVDHQVKLTVDRVNDESVLRS
                     IEMIQPEEPNGYPSSRAGVGDIYVDQNGNVVPPPENAEQRPPRRIIKAGKTTGERALM
                     DVVTEVHPGTRFTLNWLPLGGFVRPKGENDPTIPGGLAGASPWARLAVLFAGPLMNLL
                     TAVVIYSIIFTRVGVPDTNRVLVASVVEGGPAAQAGFHADDIFVSGNGTPIQSYDDLR
                     AIVDANDGNPVTFLIDRHGEQIELTATPLLNEAEQRVMIGVGLGVPFKPTTSLVDTLT
                     LGARYTYLNVRTLLS"
BASE COUNT         1446 a         1465 c         1573 g         1547 t
ORIGIN      
        1 atatagcgaa ctgcccgccc ggttgtaggt gtaaggcaag gaacgcagcg cgtaggcgat
       61 tccaccttcg gtgaggtcgc gtgtgtaggg gagatgaatg aggtcagagg ctgagatcat
      121 tcttcaaagt tttcgtgatc gtcttcctgc tcttttgata tatcccgcgc tgcgaaatga
      181 agaataatgc caatcatacc gaagagcacg ccaaaagcaa ttgccttccc tatctcacca
      241 ctgcgcacaa atgaattaag agtaaaaatc acaatactag ctacgcaaat taacattgcc
      301 caggcagcga ttgtcgcacc tgggcctgtt ttctcgttgt tataaataat cttatatttc
      361 cattttggcg tgaatcggat gggccatcgc cgtcgaaaac tggcctgcca ttgtaattgc
      421 tcgatataat cgttatcgcc aattgaatca tcttgtttct tagccattat ttcattgtgt
      481 ctccctattc attcctaaat tgcaaacaat aatgatgaaa cgtattgctt acccaggctg
      541 acccaagccc gaagggcgag aggcgctggt tatcctgaac ccagattttt tcgtctttca
      601 aggcatacgt cttcccaagt tcgcgtgcga tgtcccaggc taacggataa atcttcccgc
      661 ccttcttgac gtttttgaca atgatcgtga gataagcttt ttcgcgtagc aaaggtctta
      721 gtcctttgta aatatctacg agtttctcaa ggaattcgtt gtaatcacta atattcccca
      781 tatcattcgg gtcgtctgaa taatggacat ccagctcgtc agaggcgcga cgcttctttt
      841 gagtctgtgc ccctcgggcg cgaagcatgt cccagtaagg aggagacgtt agcacataat
      901 cgatcattgg aaattggtaa tttgcgactt gagcagcatc tccattcaca atttcagatt
      961 ttagattttg gattgagtcg ccaagtagat tgcgttcttc ttcaataatc tgtgttgcga
     1021 tcttcgcgta ctttggattc aattcgatgc cataactgtt tcgacctgcg cgcaaggcgg
     1081 cgacgagagt cgaacctgtc ccggccatgg ggtcgagcac ggtttcccct tttttcgtga
     1141 agaattcgat gaattcctgc gccatggtct cagggaactt ggcaggatgg accagcacac
     1201 cctttttacg cggcggcggg ttatgaataa accatgattt ctggaatttc agccattctt
     1261 tggacgtgag gtcgttgagt ttgtttttca tattattgtg tgttcgcgct gagcggagcc
     1321 gtaggcgtag acgaagtgca gatttgccaa gatatatgcg cttcgtctgc ggtcttcatt
     1381 tcactacaac ctccgctcag cgcgaaaatt atggcaaagg cgtgcttgtc ggcggcacat
     1441 aatttacatc atacactttt aggtctgaaa aggtaacgct catgggtgta tcagactccg
     1501 aacgtacaaa gacgccgatt gcgccgctgg ggaaactttt atctttgata ccgaactgat
     1561 aacgatcgtt gaggaaaagc ctcatctcgc tgccgactgc ccagagtcca atccgcactt
     1621 ctccgggtgc gccgcgtggg gcatccccac ttgggagagg gtcaacgagc ggcagtcgga
     1681 tgcctccgtt cactcgctct gcgcgcacca gagagttaca ggacaggaca aaacgataat
     1741 aggaactgcc tacgctgcgc acgataatcc cgtaattatc ggtcccgcgg cacaggctgg
     1801 gacgcgcggt gatctcggca taaaaatttc cgaaggtgcc ctcccgacgc atactggcca
     1861 ggtagactcc aggctggatg gctaaactca accggttccg gctgatggca gcactgcctt
     1921 gatctgagac cgctgtgtcc cacaacttca tgtctgaaaa attatcggta aggttttcag
     1981 actccacacc cggtttcatc tcaggtgtcg cagtgtaagt ggggaaggtt aggtgtgtag
     2041 acgtcgcaga tggaggaaac cagacgatgg tgggggttgg cgttgaagtg tcgattggca
     2101 ggggagtcgc ggtgggcgga gttgagtcga acggcaggca cgctccagtg agtaaggtca
     2161 ttgcgaggag gatccaagca acgacacagc aatttcgtat acggaggctg atatggcttc
     2221 gccttacggc tcgcaaggac gaggagtaaa ataacatcaa atcattttac cgcttgtatc
     2281 acgcatgtcc aacatgtgtt tgctaaactt tcgaattaaa ttataggctg attttcgtat
     2341 ctaaagttta gatcattaag cggcactttg tagcacgtca ggagacttta tgaccgaaca
     2401 gcgagcgcag cgagtgctgg tgacgggcgc atgtggtgag attgggcagg ctctgattca
     2461 ggcattggcc cgcaggggtg gttatgaaat cgtaacttca gacctctcgg ctctgccaga
     2521 cagtatcaaa gcgttgtcgt ttgagcacgt gcagggggat ctggtttata aggtcaagaa
     2581 tttttatgat tatgatttcg atgtgatctt tcatcttgcc gcctcgcttt cctccaaggc
     2641 agaagtggcc agtgaggagg cacatcgcat caatgtggaa ggcaccatgc aactgctgat
     2701 gctggctgcc taccgttctg aaaagtatga aaaatctgtg aagtttttat ttccaagctc
     2761 gatcgccgcc tatggcatgc caagtttgga ggcaaagaca gaggctgggt cggtgacgga
     2821 acaggtttat aacacgccac aaaccatgta tgggtgtaat aaactgtatt gtgaaaaatt
     2881 ggggatgtat tacagcaagt tctatggtca aaaacatttg gatgaagagc ctccctgcat
     2941 gctggacttt cgctcgatcc ggttccccgg cttgatctcc gcttcgacgc tccccagcgg
     3001 tgggacaagc gactacggtc ctgaaatgct ccatgcggct gcgcagggaa aaccctatac
     3061 ctgttttgtc cgtgaagata cgaagatctc ttttatggcg atgccagatg cgatcaaatc
     3121 tctgctgatg cttctggaag tgccgcgcga gaagctgaac aaccaggtgt ataacatcgc
     3181 ggccttcgcc ctgaccgcca gtgaattccg cgatcgcgcg gtcagggcct ttcccggtgc
     3241 acagatcacc ttcaagccca acccgcgtcg ccagggaatc gtcgattcgt ggcccgaaga
     3301 tgtggatgat tcgctggctc gttcagagtg gggctggaaa cctgactacg acgcagacgc
     3361 gttctttgat gattactttt tgcccgagat tcgaaagcga tatggtaaat aaggtgactg
     3421 tcacttccgc gaagcgagtg acagtcacct aaatgttata atcctcgccg atgaaaattt
     3481 tcaccgcact tgttgccttt ttgtttgttt ttgtcttgat tttcattagc acgattccct
     3541 ctccgactgt tgtgaacgcc gcgccgaacg cgcagtttgt cacggccacc ccgggaccag
     3601 acggacggat tctgtataca gttgtggatg gagataattg ttcgatcgtg gcattcaacc
     3661 acggcatcac cgtgcctgac ttgcgtgcac taaatacccg tctcgatgaa aactgtactc
     3721 tgacggttgg tcagcaaatt gttgttgggc ttgcccagcc ggaaggccct acagccggac
     3781 ccggacccac gctcccaccg gccactgtca cggcgacgcc cgtgagcggc acaacggaag
     3841 tctgtgtgtt gctattcgac gatatgaacg gagatgcact gcgtcaggag acagagtttg
     3901 ggatcgaggg cggcgccgtc agcctcacca atctgaacgg gtcttattct gaaacgcaga
     3961 atacgacggc cgccattgac ccggacctcg tcgagccaat ccgatcctgt tttattgatg
     4021 ttccgcaggg agaatataac gtcagtatcg cggtgcccga tacctataac ccaaccatgt
     4081 tattaagtta tacacttacc gtcagagcag gtgaccgcgc ttcgattgat tttggtgcgc
     4141 aatccaagac ggttacggta agcgaaacga cagacacaca gggcggaaga tcgtctatgc
     4201 tgggtatctt tggtcttctc ttactgctca gtggcgccgg gcttggatat tatgcctatc
     4261 gttccggtca gccgaggagc aagttaaagg gaagtccgtt gacgaagaga taatatccct
     4321 caccccggcc ctctcccaat aggagaggga gttattaccc catcggatat ctcattcgtt
     4381 gtccaccgat taaatgcaga tgcaggtgaa gcacagtctg cccaccatcg acgccggtgt
     4441 ttgtgatcag gcgatagccg ccgtttgaaa tcccttcctc gtttgccaat tgactcgcca
     4501 ccgtgaaaag atggcccatt agtggttcat cttctaattt aaggacgctg accgattcaa
     4561 tgtgcctgtt cggtacgatc aggatgtggg tcggtgcgac ggggttgaga tctcggaacg
     4621 ctgtcacctg gtcatcgcga aagacaattg tagatttggc ttcccctgaa acgattttac
     4681 agaagataca agaggatgtc atttttgatt tgatccttga ataacgggca ataacgtgga
     4741 tgagaacagc cctgcatcgt gctacaaaca gccgaaactg cgcggcagag tttagggtag
     4801 gaattttggg ctgcaactta atccataagc ctgtatataa tgaaatcgga taatacgcat
     4861 aatttaccat aacaggcgtc ggattggctt cgtaatcaat atatatgaag gatattagaa
     4921 atatcttacc ttgtcagggc gtcccagtaa aaggcgatat aatgcacgcg cgaaggaaaa
     4981 atttatgaac gaaacctctt taactttact gatatttatc cttgcctttg gtggcatggt
     5041 tatcattcac gaatttgggc actatattgt tgcgcggcta ttcaaaatcg aagtggagga
     5101 atttggtgtt ggtttgccga cgcccggagc cttgaccctc tggattaaca aaggatattt
     5161 gcttctggat gatggcggaa gaatagagat cccgtacaac tttaaattgc ctgtcaactg
     5221 gaatcagctg gtggaccatc aggttaagct cacggtggac cgagttaatg acgaatcggt
     5281 cctgcgttcc attgaaatga tacagcccga ggagccaaat ggatacccca gttcaagggc
     5341 gggcgtgggc gacatctacg tggatcagaa cggcaatgtc gttccgccgc cagaaaacgc
     5401 cgagcaacga cctccccgaa gaatcatcaa agccgggaaa acaacgggtg aacgagcctt
     5461 gatggatgtt gtcacggaag ttcatccggg cacgcgtttc accctcaact ggcttccgct
     5521 gggtgggttt gtgcgtccga aaggggaaaa cgacccaacc atccctggtg gtctcgcagg
     5581 ggccagcccg tgggcgcgtc tcgcagtatt gttcgccggt ccgttgatga acctgcttac
     5641 cgcagtggtc atttacagca tcatctttac ccgtgtggga gttcccgaca caaaccgagt
     5701 gcttgtcgct tcggtcgtcg aaggcggacc tgctgcccag gctggatttc atgccgatga
     5761 tatttttgtt tccggcaacg gcacgcccat tcaaagttat gatgatctgc gtgccattgt
     5821 ggatgcaaac gatggcaatc ctgtgacgtt tctcatagac cgtcatggag agcaaattga
     5881 attgactgcc actccgctgc tgaatgaggc agaacaacgc gtcatgatcg gtgtcgggtt
     5941 gggtgtccca ttcaaaccta ctacgtcgct ggtcgataca ttgaccctgg gcgcacgcta
     6001 tacctacttg aacgtgcgaa cgcttctatc t
//
LOCUS       DASTJP010000118         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_242207, whole genome shotgun sequence.
ACCESSION   DASTJP010000118 DASTJP010000000
VERSION     DASTJP010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242207"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..>2295)
                     /locus_tag="VFQ23_03735"
     CDS             complement(138..>2295)
                     /locus_tag="VFQ23_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HET9905718.1"
                     /translation="LTEDTFDPKKQYHKETIFTIGNGYLCTRGAFEEGYPNDHRATFI
                     HGVFDAAPIVFTELANAPDWLPLTVYLNDERFSLDSGTIETFQRNLDLRTGVLTRTVR
                     WRSPSGLVATFTFERFASLANEHLLYIRCRVTPEFTGTIEFRAGLNGNMHNEGLVHFE
                     WLGQGKQENIFYLHNRSRKSGINVATAMQITNIIGNAAGSEDWDVQNAPTQMIRLEAL
                     PGQTVGVDKHVAVFTSRDVDSSIVIHSAVSQVRESTGWESALESSKQAWAEEWNRTDV
                     IIEGDEEAQIAIRFNLFQMLIAAPRHDDRVNIGAKTLSGFGYRGHAFWDTEIFMLPLF
                     IYTAPHIAKNLLNYRYNNLPGARRKAKGNGLEGAQFPWESADTGDEVTPTWVTHFADP
                     TKLVRIWTGDIEIHISADIAHAAYQYWQATGDDEWFIEKGIELILDTAKFWASRAEWN
                     TEEKRYEYNDVIGPDEYHEHVDNNAYTNRMAQWNLQTALETLEWLKTQAPHKANELIS
                     RLDLNEDRLSKWRDVIEKIYLHIEPSGLIEQFEGYYQRKEVDLTALEPRTKSAQEIFG
                     IEGCNETQILKQPDVLMMQYVLRDHYSDEAVRVNYDFYNPRTDHTYGSSLGPSIMAIM
                     ACDVGKPEDAYEHFIRAVRADLRDVRGNAGDGIHAASAGGTWQSVIFGFGGLRVTQDG
                     WQTNPRLPEHWKRLAFKFFHRGQMHEVDLMNESEQT"
BASE COUNT          527 a          572 c          595 g          601 t
ORIGIN      
        1 ggcgggaggc ggggtaggtg aaggggttgg taagaattgt gtggctgttg ctgacggcgt
       61 ggttgcagtg gctgtactgc atgccgccaa taaaaacgac aggatcaaaa tgagccgagt
      121 gacctgacct agtaagttca cgtctgctca ctttcattca tcaaatctac ttcatgcatc
      181 tggccgcgat gaaaaaactt gaatgccaat ctcttccagt gctctggcag gcgaggattt
      241 gtttgccacc cgtcttgtgt gacacgtagt cctccgaaac caaagatgac cgattgccat
      301 gtgccgcctg cggaggctgc atggattcca tccccggcat tgccgcgcac atcacgcaag
      361 tcagcccgca cggcgcggat gaagtgttca tacgcatcct cgggtttgcc gacatcgcaa
      421 gccatgatcg ccataataga tgggcccagc gatgagccgt aggtgtgatc tgtgcgcggg
      481 ttatagaaat cataattcac ccggacggct tcatccgaat agtgatcgcg caaaacgtac
      541 tgcatcatca gcacatcagg ctgcttgagg atttgcgttt cattgcagcc ttcgatacca
      601 aaaatttcct gggccgattt tgtgcgcggc tccaaagcgg ttagatcaac ctccttgcgt
      661 tgataatagc cctcgaattg ttcgattaag ccagatggct cgatgtgaag ataaattttc
      721 tcaatgacat cacgccattt gctgagtcga tcttcgttta ggtccagtct cgagatcaat
      781 tcgttggctt tgtggggagc ctgggttttc agccattcca gtgtttcgag ggctgtctgc
      841 aaattccatt gtgccatgcg attcgtatag gcattattgt ctacgtgctc gtgatattca
      901 tcaggaccga tcacgtcgtt atattcataa cgtttttctt ctgtattcca ttccgcgcgg
      961 gaagcccaaa acttcgcagt atcgaggatc agctctatcc ccttttcaat gaaccactca
     1021 tcatccccgg ttgcctgcca gtattggtac gcggcgtggg cgatatcagc agagatatga
     1081 atctcgatat ctccggtcca aatgcggacc agtttggtcg ggtctgcaaa atgggttacc
     1141 caggtgggtg tcacttcatc gccggtgtcc gcgctttccc aggggaactg cgcgccttcc
     1201 aagccattgc cttttgcttt gcgccgcgca cccggtaaat tgttatagcg gtaattcaac
     1261 aggttctttg caatatgtgg cgcggtatag atgaacaggg ggagcatgaa aatttcagta
     1321 tcccaaaagg catgcccgcg atagccaaag ccggataggg ttttggcgcc gatatttacg
     1381 cgatcatcat gacgcggcgc ggcgatcaac atttggaata agttgaagcg gatggcgatt
     1441 tgcgcttctt cgtcaccctc gatgatcaca tcggtgcggt tccattcctc cgcccaggcc
     1501 tgtttgctgg actccagagc ggattcccac ccggtgcttt cacgtacctg actgacggct
     1561 gagtgaatga ctatggatga atcgacatcg cgagaggtaa atacagctac atgtttatca
     1621 acgccaactg tttgacccgg aagtgcttct aggcggatca tttgagtcgg cgcgttttgc
     1681 acatcccaat cttcactccc cgccgcgttc ccgatgatat tcgtgatctg catggcggtc
     1741 gccacattaa ttccagattt ccgggagcgg ttgtgcaaat aaaagatatt ttcttgctta
     1801 ccttgtccga gccattcaaa atgaactaat ccctcattgt gcatgttgcc attcaagcct
     1861 gcgcggaatt caattgtccc ggtaaattca ggggtaacgc gacagcggat atagagcagg
     1921 tgctcatttg ccagcgatgc gaagcgctca aaggtaaacg tagccaccag ccctgagggt
     1981 gaacgccagc ggacagtacg agttagtacc cctgttcgaa gatcgagatt cctttgaaac
     2041 gtctcaatgg tacctgagtc cagagagaat cgttcgtcat ttagataaac agttagtgga
     2101 agccaatcgg gcgcgttggc gagttcggtg aagacaatcg gcgcggcatc gaacacgcca
     2161 tgaataaatg tagcacggtg gtcatttgga tagccttctt caaatgcgcc gcgcgtacaa
     2221 agatagccgt ttccaatcgt aaatatggtt tctttgtgat attgcttttt agggtcgaac
     2281 gtgtcttcag taagc
//
LOCUS       DASTJP010000119         4747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_541463, whole genome shotgun sequence.
ACCESSION   DASTJP010000119 DASTJP010000000
VERSION     DASTJP010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541463"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1276)
                     /locus_tag="VFQ23_03740"
     CDS             complement(191..1276)
                     /locus_tag="VFQ23_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET9905719.1"
                     /translation="MAQKKVRVAIVGVGNCASSLVQGVQFYKDTPDDAQVPGIMHTRL
                     GGYHLRDIEFTLGIDVNVTKVGKDLSEAIFAEPNNTYKFSEVPNLNVPVVRGMTHDGL
                     GKYISGMVKKAPGSTADIVKLLRDTKTDVVVNFLPVGSEMATKWYVEQCIEAGCAFVN
                     GIPVFIASSEYWAKRFRDAKLPVLGDDVKSQVGATILHRSLATLFVDRGVKIDRTYQL
                     NFGGNTDFLNMLERERLESKKISKTNAVTSMIPYELDHNDVHVGPSDYVPWLSDRKWA
                     YVRMEGTTFGNVPLNVEVKLEVWDSPNSAGVIIDAIRSAKIALDRKLSGPIIGPSSYF
                     FKTPPKQFRDDICRAKVEAFINGDSTE"
     gene            1358..2623
                     /locus_tag="VFQ23_03745"
     CDS             1358..2623
                     /locus_tag="VFQ23_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9905720.1"
                     /translation="MTTNQRIDHLERLLEVVRGLTTAPDLEAFLQSIISEATELTNSE
                     LASILEYDETAEELRFLAVHWFQRDMLRPLGVPLEDSAAGWVYRKGQPLIIQDVKVDK
                     RHFKVIDRVTKHVTHSLAAVPLMVRGEVVGVLEALNKKDDAHYTEEDLTILATLAALA
                     AQAIKNATLQRKVKAATIELAELERLKADFIAITSHELRTPLGLILGHATFLQELTGS
                     EYTEQLDTIIRNATKLKDIVENLSDVDNIQSGAARVRSQKASLAKLAEDVISSFQEEA
                     NSRNITLKLEKGESPYEIDADGGKVSIALSNLVKNAIQYTDSGGRVLVKLEEDSGYFK
                     VAVKDEGIGIPARDLPRVFERFFQVESHLTRRYGGMGLGLTVAKAMIELHGGRIWAES
                     EVGKGSTFTFLLPVNQMPEQVSSTNPFIE"
     gene            2699..3691
                     /locus_tag="VFQ23_03750"
     CDS             2699..3691
                     /locus_tag="VFQ23_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET9905721.1"
                     /translation="MAPSFLVFGQLTREYLLPPSGPPRLDAMGGSLFYGAAGLGVWES
                     DIGLVGRAGNDYPRQWLNECKARGLDTRGIHIVQKDLDVREFLSYSESFEVSRINPVT
                     QFARREMTFPKSLLGYQPLDEKKTDEERALIVTDIPEEYLNARAALICAMGLIPQTQL
                     IAGLKRGSVHTFVLAPSASTMTPTARRALPALLNGVTAFVTSQEELKNLFWGETHDLW
                     KMAEAVSMYGCEYVVIKCGARGQLLYDVDTKRKWEIPAYPARLADPTGAGDAFCGGFL
                     AGYCKDYDPIEGVLYGNISASLKLEGSGPFYPMDVMPGLAEARLHVLRDMVREV"
     gene            3776..>4747
                     /locus_tag="VFQ23_03755"
     CDS             3776..>4747
                     /locus_tag="VFQ23_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9905722.1"
                     /translation="MNEIIKGIVDLAVDIQQIPAPTFAERKRAEFICDLFAKERLVDV
                     FIDELGNVFGRLKTKGRRKPLIVSAHLDTVFPQETILDGKRAGSKVYGPGIGDNSIGV
                     AALVGLTRMLREQNVHTSGDVWFVANVCEEGLGDLRGMKAVVDRFGSEAHAYLVLEGL
                     ALGHIYHRAVGVKRYRVTARTSGGHSWSDYGKPSAIHELSKLVVQLAELDLPKEPRTT
                     MNVGKISGGTSVNVIASEARLDLDLRSDDEKSLGELVSAAETLIQSANKPGVSVEADV
                     IGQRPAGEISVNHPLVRLAKDCLGEQGLEGGLISGSTDANVPLSKGYP"
BASE COUNT         1134 a         1125 c         1321 g         1167 t
ORIGIN      
        1 gcgctcatat cgggtgtgga ggcaggtgcc tgtggggtac aagcactaag gaaaatgatc
       61 agtaggaaca agtttgtaac acttcgaaaa gtcttttgca tgagatgatt ttatccgaaa
      121 agaatgagac ggcaggtcac ctgccgtctc actgttattg aaacccgtcg ggagtcttgg
      181 accccgaaat ttactcggtg gaatcgccgt taataaaagc ttccactttt gcccggcaga
      241 tatcatcgcg gaattgtttg ggtggagtct tgaagaagta cgaagacggc ccgatgatcg
      301 gtccgctgag tttgcggtcc agagcgatct tggccgagcg aatggcatcg atgatcaccc
      361 cggcagaatt ggggctatcc cacacttcca gtttgacttc cacattgagc ggcacatttc
      421 caaaggtagt cccttccata cgaacgtacg cccatttgcg atccgaaagc cagggcacgt
      481 aatcgcttgg acccacatga acatcgttgt gatccagttc gtaggggatc atgctggtga
      541 cagcattggt cttggatatt ttcttacttt cgagacgttc gcgttcaagc atgttcagga
      601 aatcggtatt gccaccaaag ttgagttgat aggtgcgatc aatcttgacg ccgcggtcca
      661 cgaaaagcgt cgcgagagaa cggtgcagaa tcgtcgcacc aacctggctc ttgacatcat
      721 ccccaagaac tggcaatttt gcatcacgga aacgttttgc ccagtactct gaagaagcga
      781 tgaagacggg aatgccattg acaaatgcac acccggcctc aatacattgt tccacatacc
      841 atttggttgc catctctgag ccgaccggaa ggaagttcac cactacgtcg gtcttggtgt
      901 cgcgcagtag ttttacaata tcggccgttg accccggtgc tttcttgacc atacccgaaa
      961 tatacttgcc caagccatca tgggtcatgc cgcgcacaac cggcacattc aggttgggga
     1021 cttcggagaa tttataggta ttatttggct cagcaaagat ggcttctgac aggtctttgc
     1081 caactttggt aacattcacg tcaattccca gcgtaaattc aatgtcgcgc aggtgatacc
     1141 ctcccagacg ggtatgcata attccgggga cttgtgcatc gtctggtgtg tctttgtaaa
     1201 actggactcc ctgcacgagc gacgacgcac agttccccac gccgacaatg gcgacgcgta
     1261 cttttttttg tgccatgcaa tgtttctcct ttgagttatt gataattaca acgaatcgtt
     1321 tattctattc ggtgggtcgt gttgtataat cgaagcaatg accaccaacc agcgaattga
     1381 tcacctggaa cgattattgg aagtcgtacg gggacttacc acagcacctg atcttgaagc
     1441 ttttttgcaa tccattatta gcgaggcaac cgagttaacc aacagcgaac tcgcttccat
     1501 ccttgagtac gacgaaactg cagaggaatt gcgtttcctg gccgtgcatt ggtttcagcg
     1561 tgatatgctt cgacctctgg gtgtgccact ggaagacagc gccgccggct gggtctatcg
     1621 caaaggtcaa ccattgatca ttcaggatgt gaaggtggac aaacgtcact tcaaggtgat
     1681 cgatcgcgtg accaaacatg tgacacattc tctggccgct gtgccgctca tggtccgcgg
     1741 cgaagtggta ggtgtattgg aagctctcaa taaaaaggac gatgctcatt ataccgaaga
     1801 agacctcacc attctcgcga ctctagctgc actggctgca caggcaataa agaacgcgac
     1861 ccttcagcga aaagtgaaag ccgcgaccat tgaacttgca gaactggaac ggctcaaagc
     1921 ggacttcatt gccatcacct ctcatgagct gcggactccc ctcggcttga tcctcggtca
     1981 tgccactttc cttcaggagt tgacgggttc ggagtatacc gaacaactgg ataccattat
     2041 ccgcaatgca accaagctga aggacattgt tgaaaacctc tcggatgtcg ataacataca
     2101 aagtggtgcg gcccgcgtgc gcagtcagaa agcttcgctg gcaaaactgg cagaggatgt
     2161 aatttcgtct tttcaggaag aggcgaattc caggaacatt acgcttaagc tggagaaggg
     2221 agagtctccc tatgagatcg atgcggacgg tggtaaggtc agcattgcat tgagcaacct
     2281 ggtgaaaaac gcgatccaat atacagattc aggtggcagg gtcctggtca aactggaaga
     2341 ggactccggt tatttcaaag tggcggttaa ggatgaggga attgggatcc cagccagaga
     2401 tttgccgcgt gtctttgaga gattctttca ggtcgaatca catctcactc gccgttatgg
     2461 cggaatggga ctggggctga cggttgcaaa agcaatgatc gaattgcacg gtggacgcat
     2521 ttgggcggaa agcgaagtag gcaaaggcag cacctttact tttctcctgc cagtcaacca
     2581 gatgcctgag caagtctctt cgacaaatcc ttttattgaa taaatgtagt tctctcgtgg
     2641 tttaagatcc gctcatgctg gcggattttt ttattatata tcttataatt gcgccatcgt
     2701 ggctccgagt tttttagttt ttggtcaact gacgcgtgaa tatcttctgc ctcccagcgg
     2761 tcctccgcgt ttggatgcca tgggtggcag tttgttttat ggggcagccg ggctgggcgt
     2821 gtgggaatct gacatcggac tggttgggcg tgcggggaac gattacccgc gtcagtggct
     2881 caatgaatgc aaagcgcgtg ggctggatac gcgtggcatc catatcgttc agaaggattt
     2941 ggatgttcgg gaattccttt cctatagcga atcgttcgag gtcagccgca tcaatccggt
     3001 gacccaattt gcccgccgtg aaatgacgtt tcccaaatcc ctgctgggct accagcccct
     3061 ggatgaaaaa aaaaccgatg aggaacgcgc cctgatcgta acagacattc ccgaggaata
     3121 tctcaacgct cgcgcggcac tgatctgtgc tatgggattg atcccacaga cgcaattgat
     3181 cgctgggctc aaacgtggct ccgtgcatac gtttgtgctt gcgccttctg cgtcgacgat
     3241 gacaccgacg gcgcggcgtg cgttgccagc cctgttgaac ggtgtgaccg cttttgtgac
     3301 atcgcaggag gaattaaaga atctattctg gggcgagacc catgatcttt ggaaaatggc
     3361 tgaagcggtc tccatgtatg gctgtgaata cgtggtgatc aaatgcggcg cgcgcggtca
     3421 gttactttat gatgtggata ccaaacggaa atgggagatc ccggcctatc cggcgcgcct
     3481 ggctgacccg accggcgccg gggacgcatt ttgcggtggt tttctcgcag ggtattgcaa
     3541 ggattatgat ccgattgaag gcgtgctata tgggaatatt tctgcttccc tgaagctgga
     3601 agggagtggc ccgttctatc ccatggacgt catgccgggg ctggcagaag cgcggttgca
     3661 cgtcttgaga gatatggtga gagaagtgtg atcttgtcgc cgcagacatt tcagtagaca
     3721 aagagtagtc tattgaagag aatacgaatc cagaataaag attggtttat tgaagatgaa
     3781 tgaaatcatt aaaggtattg tcgatctggc ggtagatatt caacagattc ctgcgccgac
     3841 ctttgcggag cgtaaaaggg cagaatttat ttgtgatctg tttgcgaagg aacggctggt
     3901 agacgtattc atcgatgaac tgggcaatgt atttggacgc cttaaaacaa aagggagaag
     3961 aaagccgctg atcgtctctg cacatttgga tacagttttt cctcaggaga caattctgga
     4021 tggaaagcga gcgggctcga aggtttacgg cccggggatt ggcgataact caattggcgt
     4081 tgctgcattg gtcggtttga ctcgcatgtt gcgcgagcag aatgttcata ccagcggtga
     4141 cgtttggttc gtggcaaatg tttgcgaaga ggggcttggc gacttgcgcg gcatgaaagc
     4201 cgtggtggat cgctttggct ctgaagcaca cgcctatctt gttctggaag ggctggcgct
     4261 ggggcacatc tatcaccgcg ccgtcggtgt caaacgctat cgcgtcaccg cccgtacctc
     4321 cggtggacac tcctggtcag attatggcaa accgtcagca atccatgaac tatcgaagct
     4381 ggtcgtgcaa ttggcagagc ttgacctgcc gaaggaaccg cgcacaacga tgaatgtggg
     4441 caaaatttcg ggcggcacct ctgtgaatgt gatcgcctca gaagcgcggc tggatctcga
     4501 cctgcgctct gatgatgaaa aatcgctggg ggaactggta tcggccgccg agacattaat
     4561 ccaaagcgcg aacaagcctg gcgttagcgt ggaagcggac gtgatcggtc agcgcccggc
     4621 gggggagatt tcggtgaatc atccgctcgt ccggttggcg aaggattgcc tgggtgaaca
     4681 gggattggaa ggtggtttga tctcagggtc taccgatgca aatgtgccgc tcagcaaagg
     4741 gtacccg
//
LOCUS       DASTJP010000120         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1191807, whole genome shotgun sequence.
ACCESSION   DASTJP010000120 DASTJP010000000
VERSION     DASTJP010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191807"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..609
                     /locus_tag="VFQ23_03760"
     CDS             265..609
                     /locus_tag="VFQ23_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905723.1"
                     /translation="MKKILISILLMLVLMTSCAPGSDLEVTTPQSTIELRSPGPNPQL
                     DQTDDAGRISGFVQGLWHGIIAPVSVIGSFFNPAMQMYEVHNNGRDYNLGFLIGVAFI
                     FLLLGLIGGRWR"
     gene            677..1132
                     /locus_tag="VFQ23_03765"
     CDS             677..1132
                     /locus_tag="VFQ23_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905724.1"
                     /translation="MYNGFNPLPLSPQFRRVLFIAAAQVNDNMMIPEVDMKNVLLFGL
                     LVTLSAVMAACTPSTSVSATTPDSTMLLTAPGPNPMLNQPDALGRVARAGAGLWHGII
                     APITLVLSFFNPAIQMYEVHNAGSEYDLGFLIGVTLIISLLGLFVRMRR"
     gene            1315..1533
                     /locus_tag="VFQ23_03770"
     CDS             1315..1533
                     /locus_tag="VFQ23_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905725.1"
                     /translation="MENDFDFGLEDRENFRINPDVWLHTLLAIAGDKEKKAEAVRQIV
                     QNTGFSSEQVQLIIRTTISILINQTRAN"
     gene            1984..2511
                     /locus_tag="VFQ23_03775"
     CDS             1984..2511
                     /locus_tag="VFQ23_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905726.1"
                     /translation="MIPKASGILVVFLILLALLPAQTALADTGPKPRMDFQFRQEMTG
                     EPPVTIMSGILYECDQADCSDAAPIEEVGPQGFRCEVNSCSAIAYGFAPYHRIAIEFS
                     DGKTRQSNIFETAGFESKYTVIIRPDDLIVETRFTLGGFPRTAIILTACFCAVVGVGL
                     VLGLIIFLLRRSTKN"
     gene            2508..2834
                     /locus_tag="VFQ23_03780"
     CDS             2508..2834
                     /locus_tag="VFQ23_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905727.1"
                     /translation="MIGTLLITIIIEGAVVIGYSLWKTKPVRPILFTSICGNLLTQSL
                     LWIGLNLFFRNYLVTLLMAEILIWILESIFLYFIRPNHLKLTEATLLSLVMNGFSFGL
                     GWFLPV"
     gene            2871..>3398
                     /locus_tag="VFQ23_03785"
     CDS             2871..>3398
                     /locus_tag="VFQ23_03785"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9905728.1"
                     /translation="MFEFNITARNQRARTGIFATPHGDLMTPVFAPVGTQATVKTLTP
                     EHLKDINASLVLSNTYHLYLRPGDELVRDMGGLHRFMQWQRPMLTDSGGFQVFSLAQT
                     RKIDDDGVTFKSHIDGSTHRFTPEKSIAIQENLGADIIMVFDECSDPNDHAYTKLAME
                     RTHRWAERSLQAKKRR"
BASE COUNT          811 a          875 c          849 g          863 t
ORIGIN      
        1 agcgatgatg tgttcacctg gggtgagcag ggcttttcct actattcgag ctaccctgtg
       61 gatggcgagt acgcagtcgg cattgttgcc tacgatttcg acaataactt cgtcgcaaac
      121 tttgaattta tcaactatac acgctgaccc attctgaacg gacctgtaat tctccccggt
      181 catgtcgatg attggggaga attttcatat ccgcccctcg caacggactg ttaagctcct
      241 cgttaaacag cctaggaggc cgatatgaag aaaattctga tttccatttt gttaatgctt
      301 gtcctcatga cgagctgtgc tccgggctcg gacctggagg tgaccacgcc gcaatcgacg
      361 atcgaacttc ggtcgcccgg acccaacccg cagctggatc agacagatga cgcgggccgc
      421 atttcgggct tcgtgcaggg actctggcat ggcatcattg cccccgtcag tgtgattggc
      481 tcgtttttca accccgcgat gcagatgtat gaagtacaca acaatggccg tgactataac
      541 cttgggtttc tgatcggagt cgcctttata tttctgctcc tgggcctgat cggaggcaga
      601 tggcgttaga gcccgtaagc tcgcgttgtc atactatata aacctccccg ttcggtgaat
      661 cgacggggag gttttattgt ataatggatt caacccactc ccactgagcc cacaattcag
      721 acgcgtcctc ttcatcgctg cagcacaggt gaacgacaat atgatgattc cggaggtaga
      781 catgaagaat gtacttctgt ttggcctgct tgtaacactg tccgccgtga tggcagcttg
      841 cacccccagc accagcgtgt cggctacgac gcctgactcg acgatgttgc tcaccgcccc
      901 cggccccaac cctatgctca accagcctga tgcgttggga cgcgtggcac gtgccggagc
      961 gggattatgg cacggcatca tcgcccccat aaccctggta ctctcattct tcaatcctgc
     1021 gatccaaatg tacgaagttc ataacgcggg gagtgaatat gacctgggtt tcctgatcgg
     1081 cgtgacgctg atcatcagtc ttttaggact atttgtacgt atgcggcgat gagtatagat
     1141 accagagctg cttcaaagcc tgtagtcctt gataagcagg taacgcgttt ttccgacaaa
     1201 aacggtgcac aaagtattca aacacattac atttccctaa caaagggtta cgaatcgagc
     1261 gttccaacgt catgacagat atatcatctc tttcataaga taaaaggaag acgaatggaa
     1321 aacgattttg actttggttt ggaagacagg gaaaatttca gaattaatcc tgacgtctgg
     1381 ctgcatactc ttctggccat cgcgggagat aaggaaaaaa aggcagaggc tgttcgtcaa
     1441 attgtgcaga atacagggtt ctcttccgag caggtccaac tcattatcag gacaaccatc
     1501 agtatcctaa tcaaccagac ccgcgcgaat tgattgttta tcacgttggc ttatgtagga
     1561 accgcaggca agagcctgtg gtttttgatt ttaaacaacg tgatggataa gatgtgtagg
     1621 gatataatcc agacaacagg agcaaacata actatacact attggatcag tgtcgcttcg
     1681 aaagaccatt tgcagtgcgg gctggcaggc ggctttatgc aggcaatcat ggcaaagaca
     1741 cctccctgag gcggatgcac gcaggcgatt gggtcatctt ctattcacca ggagcagagt
     1801 ttggcaggcc cgaaagactt caatgcttca cttatcaaag ataaaattca ctggggcgcg
     1861 ccatttcgat ttggagccct gcagatcccg gaagaggatt tccgtttcat agcaagcaaa
     1921 atggtgaacg atcaagactt gtcggcaaaa tagatttcat atagaaagat catcagaggc
     1981 aacatgatcc ccaaagcgtc cggaatattg gtagtctttc tgattctgct ggctcttctg
     2041 cctgctcaga ccgcgctggc ggataccggc cccaagccaa gaatggattt tcagttccgg
     2101 caggaaatga ccggggagcc accggtcacg atcatgtcgg ggatcttata tgaatgtgac
     2161 caggcggatt gcagcgatgc cgctccgatc gaagaagtgg ggccgcaggg ttttcggtgt
     2221 gaagtgaata gctgcagcgc tattgcttat ggctttgctc cgtatcaccg gattgcgatc
     2281 gagttttccg atgggaagac gcgccagagt aatatctttg aaacggcagg tttcgagtcg
     2341 aaatacacgg tcatcatccg cccggatgat ttgattgtcg aaactcgctt taccctgggt
     2401 ggatttccac gtacagccat tattcttaca gcctgcttct gtgctgtggt gggtgttggt
     2461 ctggtcctcg gcctgatcat tttcctgttg cgccgctcga cgaagaattg atcggcactc
     2521 tcttaattac gatcatcatc gagggtgcag ttgtcattgg atactcactc tggaaaacaa
     2581 agcccgtcag accgattttg ttcaccagta tctgcggcaa cctgcttacg caatccctgc
     2641 tgtggattgg tttgaatctt ttcttccgga actatctggt caccctgctg atggcagaga
     2701 tcctgatctg gatcctcgaa agcatttttc tctatttcat tcgccccaat catctgaagc
     2761 tcacagaagc tactctttta agcttggtga tgaatgggtt cagttttggg ttgggatggt
     2821 tcctacccgt ataacccctc gtccctcact ctcccgttat aattccctgc atgtttgaat
     2881 tcaacatcac agccagaaat caacgtgcgc gtactggtat ttttgccacc ccgcatggtg
     2941 accttatgac accggtattt gcacccgtgg ggacacaggc gacagtcaag acccttaccc
     3001 cggaacatct caaggatatc aatgcgtccc tggtcttgag caacacctac catctctatc
     3061 tccgccccgg cgatgagctg gtgcgcgata tgggcggctt gcacaggttc atgcagtggc
     3121 agcgtccgat gctcacggat tcaggtggtt tccaggtttt ctctctcgcg cagacccgca
     3181 agatcgacga tgacggtgtg acgttcaaaa gccacattga cggttcgacg catcgcttta
     3241 cgccagaaaa atccatcgcg atccaggaga acctgggtgc agatatcatc atggtattcg
     3301 acgaatgctc cgacccgaac gaccatgctt acaccaaact cgccatggag cgcacccatc
     3361 gctgggcaga acgttccctg caggcgaaga agcgccgc
//
LOCUS       DASTJP010000121         5754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_910681, whole genome shotgun sequence.
ACCESSION   DASTJP010000121 DASTJP010000000
VERSION     DASTJP010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910681"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFQ23_03790"
     CDS             <1..211
                     /locus_tag="VFQ23_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905729.1"
                     /translation="EGTEEPLRIYLSCYLALKQNNDPRSAAVLRSASEFLQDQLAKIQ
                     NSEIRHNYVANVPWRRAIADEADLR"
     gene            complement(218..595)
                     /locus_tag="VFQ23_03795"
     CDS             complement(218..595)
                     /locus_tag="VFQ23_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905730.1"
                     /translation="MMAQVNPSNGNKYGLGGPKDVDLDGINSNNYDVFTDDDKLQSAK
                     DAYPDFDWFVSYTIKDKAGNNVRNLAEYSLKFDRPDRGTLYYFLDGTAHPINYEDDDD
                     KGNKKRAKARLTVGDPPVGIGGG"
     gene            668..1225
                     /locus_tag="VFQ23_03800"
     CDS             668..1225
                     /locus_tag="VFQ23_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9905731.1"
                     /translation="MYILFNKPYGVLSQFTDEGTGHPTLKQYIDIPNVYAAGRLDRDS
                     EGLLLLTDDGKLIKRLTDPKHHIEKTYWVMIEGEPTQEKLAQLERGIRLKGYVTLPAK
                     ARLIPTPDLPPRSKPVTPHGPTVWIEIKLREGKKRQIRHMTAAVGLPTLRLMRVAIGP
                     IQLDTLKVGKWRKLTQDEINLLTSA"
     gene            1252..2259
                     /locus_tag="VFQ23_03805"
     CDS             1252..2259
                     /locus_tag="VFQ23_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9905732.1"
                     /translation="MQWSLIRLSIVVSLTILSGLPTTTPMLAQSAYQPLAAVTPFDLI
                     NAVNQLRISYGLPPYSVNSILMETAQAQAEFMASTGDVTHSGPEGIGLTQRLLAAGYP
                     LAGDISAGGFRAENISSGNEDRTAQSVVEGWSGDALHLNTMISPNLSEIGAGVAVANG
                     RVYFVIDCALPTSSALPAAVTSIPGTDASTDVSEVPTSAIVLSTPNSNGDVIHEVKPG
                     ESLWQLAIAYDVRIDEIKRDNNLLDDNIYPGNTLVIKRAVILTTPSQVATASSPVPQT
                     LVPSTPILFSETEVASWSSEGSRQEENDSGLYIAIGIITLALLGGGVVTWWGNRTKKD
                     I"
     gene            2448..2957
                     /locus_tag="VFQ23_03810"
     CDS             2448..2957
                     /locus_tag="VFQ23_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905733.1"
                     /translation="MLRKFFSWLRKPLPNVLYMEMRSQMFRLNPEKVGIKRVNNQTKV
                     WGVMTEFTIDDGYVTMVSLANGRTYMYFSSGSGILGAGDYSMVSIASAELVRTAERFV
                     SLMKPTKEHPFPSPGHTRFYIMTYDGLYTKEVLESQMDGEHTNPMYGLYAFTQNVITQ
                     IRLISSRSK"
     gene            complement(3032..>5754)
                     /locus_tag="VFQ23_03815"
     CDS             complement(3032..>5754)
                     /locus_tag="VFQ23_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905734.1"
                     /translation="LPMAIPFPIKWLESRLHIAIPTDSASVAALTQTDFLYMGLFVVI
                     AFAISIAIGMFWNRNWWKYATLFWGAFTIFYTTVFTNGPGFFTGLLGSLGYWLVQQGV
                     ERGSQPEYYYVLVQIPIYEFLPAMGSIVAIAIGLRKLAEQRIARLDEEAEVVEVQPDP
                     EPALVSEPEESETSNSIFGPFFGLLVWWVFSSILAFTIAGERMPWLTYHMAWPMILLA
                     GWGIGQIVESVVPQLAESRLQQNALAILILVVFVLAAFNTIRSLYGATPPFQGTELLQ
                     LQATSAFLFPLITTLASGALLAYLMKDDLLSISIVALALLAAITFFSSMINGASVLSL
                     SATPEMDPATLSSSQVRFGAALLALIASIFGIVYLSRQPRRSAFLNLVALTVFGLLAV
                     QTARTSFRAAYIFYDDATEYLVYAHGATGVKDVMRQVEEISERTAGGLNAVVAYDASA
                     PDTGVSWPMVWYLRDFTALRSFDQPTRSLRDSVAVIVDQKNFDKIEAALGDGFYRFDY
                     IRMWWPNQDYFNLDRPRVLNAITNPGIREGIFDIWFNRDYTQYAQAIGSNSMTLTTWQ
                     PADQMRLYVRKDVASQIWNYGVGPSEAPTTVDPYEAGIITLAADQIFDSGRFAPLGLQ
                     APRSIAPGLNDDLYIADSRNHRILHISSDGNLLQEWGTFADLALGDAPPGTFNEPWGV
                     AVGPDGSVYVSDTWNHRVQKFTADGEHILTWGQYGQPIPDVPESNSSFWGPRGIAVSP
                     QGQVYVADTGNKRIVVFDEDGNYITAFGSAGLDPGQFDEPVGVAVAPSGTVYVADTWN
                     QRIQAFLPGEDGLTYFPTLQWDVNAWFGQSLDNKPFIAVGPDEHVFISDPEGYRVIEF
                     TDTGEFVRTWGAFGVGPEEIGLASGIAVDQQGSIWLSDSGNNRILRYTLP"
BASE COUNT         1486 a         1488 c         1425 g         1355 t
ORIGIN      
        1 ggaaggcact gaagaaccgc ttcggattta cctgtcctgt tatttggcgt tgaagcaaaa
       61 caatgatccg cggtcagcgg ctgtgcttcg atctgcctct gaatttctcc aggaccaact
      121 cgcgaagatt cagaatagcg aaattcgcca caattatgtg gcgaatgttc cttggcgacg
      181 agcgattgca gacgaagcag atctcagatg aacacggcta accgccgcca atccctactg
      241 gcggatcgcc aacagtcagc ctagcctttg ctcttttctt gttgccctta tcatcgtcat
      301 cctcgtagtt tataggatgc gctgtgccat ccagaaaata atagagagtt cctctatcag
      361 ggcgatcaaa ttttagtgaa tattcggcaa ggtttcgaac gttgtttcct gctttatcct
      421 taatggtgta cgacacaaac cagtcgaaat ctggatacgc atccttcgcg ctctgtaatt
      481 tgtcgtcgtc tgtgaaaaca tcataattat tcgagttgat gccatccaga tcgacatcct
      541 tcggaccgcc gagcccatac ttgtttccgt tactggggtt tacctgtgcc atcataatct
      601 ccttcctggg tgatggtgaa tataataagg taaattatat tacaaattca tcattctatc
      661 gcttctgatg tatattctct tcaataaacc ctatggcgtg ttgtcgcaat tcacggacga
      721 aggcaccggg cacccgacgc tcaaacaata tattgatatt ccgaatgtgt acgcggccgg
      781 ccgccttgac cgcgatagcg aaggtctatt gttactcacc gatgatggta agctcatcaa
      841 acggctgaca gacccaaaac accacatcga aaaaacgtat tgggtgatga ttgagggcga
      901 accaacgcaa gaaaaactgg ctcagcttga gcgtgggata cgattaaaag gttatgtgac
      961 actgcccgcg aaagcccgcc tcattccgac tccggacttg ccgccgcgct ccaagcctgt
     1021 gactccgcat ggtcccactg tgtggattga gatcaaattg cgggaaggca agaaacgtca
     1081 gatccgccat atgaccgcgg cagttggttt gccaaccctg cggctgatgc gtgtcgcgat
     1141 tggaccgatt cagcttgata ctctgaaggt cggcaaatgg cgcaaattaa ctcaggatga
     1201 gataaatttg ctcacgtcgg cataatagcc tccaagcttt ataatatttt tatgcaatgg
     1261 tcgctgattc gattgtctat cgtcgtatca ctcacgatac ttagcggact accgacaaca
     1321 actcctatgc tggcacagtc tgcctatcaa cctcttgctg ccgtcactcc attcgacctg
     1381 atcaacgcgg tcaatcaact tcgcatttcg tacggactgc cgccatacag tgtcaattcc
     1441 atccttatgg agaccgcgca ggctcaggca gagtttatgg cgtcgaccgg cgatgttact
     1501 cactctggtc ccgagggaat cggcctgacc caaaggcttt tagctgccgg atatcctttg
     1561 gccggtgata tttcggccgg tggtttccgc gcggaaaaca tctcctcggg gaacgaagat
     1621 cgaaccgctc agtctgtagt ggaaggttgg agcggagatg cgcttcacct gaacaccatg
     1681 atctcaccaa atctgtccga aatcggtgcc ggcgtggcgg ttgccaacgg ccgggtgtac
     1741 ttcgtgattg attgcgcact gccgacctcc agtgcgttgc ctgccgccgt tacttcgatt
     1801 ccagggacgg atgcatctac cgacgtgagc gaagtaccga cttccgccat tgtccttagc
     1861 acaccaaatt ctaacgggga tgtgattcat gaagtgaagc cgggtgagtc gttgtggcag
     1921 cttgccattg cctatgatgt acgaattgac gaaattaaac gcgacaataa tttgctcgat
     1981 gataatattt acccggggaa tacgttggtg atcaagcgtg cagtcatctt gaccacgccc
     2041 tcacaagtcg ctacggcatc ctcccctgta ccacaaacat tggtgccttc gacgcctatc
     2101 ctattttccg aaaccgaagt cgcctcgtgg agttctgaag gctcccggca ggaagaaaac
     2161 gactcgggcc tgtatattgc cataggcatc attaccctgg ctttgctggg aggcggagtt
     2221 gtgacatggt ggggaaatag gacgaaaaag gacatttaat cggctgtaac tgactgttat
     2281 gtgtctgtaa tctcggcgaa aaccctcttt gatatataat ttgtttggtg ccgccaagtt
     2341 ttcgctatac agatttcccc gcaacgtatc aaaagtaact gtcatcaatt taaacggcaa
     2401 gcattctctc ttggcgagag aaaatttcat ttttggatgg gtacacgatg ctaagaaaat
     2461 tcttctcctg gctcagaaaa ccactgccca acgtactcta catggaaatg cgcagtcaaa
     2521 tgttccggct taacccggaa aaggtgggaa taaaacgagt aaacaatcaa accaaagtct
     2581 ggggagtcat gacagagttc accattgatg atggctacgt gacgatggtc tctctggcaa
     2641 atggacggac gtatatgtat ttcagttcag ggagtgggat cctgggcgcc ggtgattact
     2701 cgatggtctc cattgcttcc gccgagctag tacgaactgc ggaaaggttc gtatctctta
     2761 tgaaacccac gaaagagcat ccgttcccct ctccgggcca cacacggttt tatatcatga
     2821 cgtatgatgg gctttatacc aaagaggttc ttgagagtca gatggacgga gagcatacaa
     2881 atcccatgta tgggctgtat gccttcacgc aaaatgtcat cacacagatc cggttgatct
     2941 caagcagaag taagtaaacc tcaaattagc cgagaaacga aaaacgcctc ttctggaaaa
     3001 gaagaggcgt tttgttttga agaaatactt cttaaggcaa cgtatatcgc agaatacgat
     3061 tattacctga atcacttaac catattgacc cttgctggtc gacagcaatg cccgatgcta
     3121 aaccgatctc ttccggtccg acaccaaacg cgccccaggt gcgcacaaac tcacctgtgt
     3181 cagtaaactc aatcacccgg tagccttcgg gatcagaaat gaatacgtgt tcatcaggac
     3241 ctactgcgat gaaaggctta ttatccagag actggccgaa ccaggcattg acgtcccact
     3301 gcagcgtggg gaagtacgtc agcccatctt cacctggaag gaaagcctga atccgctggt
     3361 tccaggtgtc tgccacatag actgttccac tgggcgcgac cgccactcca actggctcat
     3421 caaattgacc ggggtcgaga cccgcactac cgaaggcagt aatgtaattt ccatcttcat
     3481 caaagaccac aattcgcttg ttaccggtat ccgccacata gacctgcccc tgtgggctta
     3541 cggcgatccc gcgcggaccc cagaaagaac tatttgactc tggcacatct gggattggct
     3601 gaccgtattg accccacgtc aggatgtgtt cgccatctgc cgtaaatttt tgaacacggt
     3661 gattccacgt atcgctgacg tagacagaac catccggtcc gactgctacg ccccagggtt
     3721 cattaaaggt tccaggtggg gcatcaccta aggccaggtc tgcgaaggta ccccattcct
     3781 gcagcaggtt gccatccgat gaaatatgca gaattcgatg atttcgtgaa tcggcgatat
     3841 atagatcatc attgagcccc ggggcaatgg aacgcggcgc ttggagtcca agcggtgcaa
     3901 atctacccga gtcaaaaatc tggtccgcgg caagggtaat gattccagct tcatagggat
     3961 caaccgttgt cggcgcttcc gaaggtccca ctccatagtt ccagatctgc gacgccacgt
     4021 cctttcggac atagagacgc atttgatcgg ccggttgcca ggtggtgaga gtcattgagt
     4081 tgctccctat ggcctgtgca tactgtgtgt aatcgcgatt gaaccagata tcaaaaatac
     4141 cttcgcggat gccgggattg gtaatcgcat tcaaaacgcg cggacggtcc aggttgaagt
     4201 aatcctgatt cggccaccac attcggatgt aatcaaatcg ataaaagcca tcaccgagcg
     4261 cagcttcgat cttgtcaaaa ttcttttgat ctacgatcac ggcaactgaa tcgcgtaggg
     4321 agcgagtggg ttgatcaaac gaccgcaatg ccgtgaaatc gcgcaaatac cagaccatgg
     4381 gccatgaaac cccggtatca ggcgcgctgg catcataggc gaccactgcg ttcagtccac
     4441 cggcagtccg ttctgagatt tcctccacct gcctcataac atccttcacc ccggttgcgc
     4501 cgtgtgcata aacgagatat tcggtggcat catcatagaa aatataagca gctctgaaag
     4561 acgtgcgcgc cgtctgcaca gccaacaagc cgaagaccgt aagcgctaca agattcaaaa
     4621 atgcacttct acggggctgg cgtgaaagat atacgattcc aaagatactc gcgattaagg
     4681 cgagaagcgc agcaccgaat cggacctggc tggaggagag cgttgcagga tccatctcag
     4741 gtgtggctga tagcgacagc accgatgcac cattgatcat gcttgagaag aacgtgatcg
     4801 cggcaagcaa cgctaaagct acaatgctga tgctgagcag atcatctttc atcaaatagg
     4861 ctaatagagc accggatgcc aaagttgtta tcagcgggaa cagaaaagcg gacgtggcct
     4921 gcagctgtaa caactctgtt ccctggaagg gcggcgtcgc gccgtagagt gaacggatgg
     4981 tgttgaaggc tgccaaaacg aacacaacca atataagaat tgcaagggcg ttttgttgta
     5041 gtcgactctc tgccaattga ggcacaaccg attcgacgat ttgcccgatc ccccagccgg
     5101 ccagcaatat catcggccat gccatatgat aggttagcca tggcatacgc tcacctgcaa
     5161 ttgtgaaggc caggatgctg ctgaataccc accaaaccag gagaccgaag aagggtccaa
     5221 agatggagtt gctggtttca gactcctcag gctcggaaac tagtgctggc tcgggatccg
     5281 gttgtacttc aactacttct gcttcttcgt cgagtcgggc tatcctttgt tcagcgagtt
     5341 tccgcagccc gatggcgatc gcgacgatcg accccatggc aggaagaaac tcatagatcg
     5401 gaatctgaac caggacgtaa taatattccg gctgactgcc gcgttccact ccctgctgca
     5461 ccagccagta cccaagcgac ccaagcaagc cggtaaagaa cccgggtccg ttggtgaaca
     5521 cagtggtata gaagatcgtg aacgcacccc aaaacaatgt tgcgtatttc caccaattcc
     5581 gattccaaaa cataccgatc gcaatagaga tcgcgaaagc gatgacgaca aagagaccca
     5641 tgtataagaa atccgtctgt gtgagcgcag caacggaagc gctgtccgta gggatggcga
     5701 tgtgaagcct ggactccagc catttgattg ggaagggaat tgccataggc aaga
//
LOCUS       DASTJP010000122         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_29714, whole genome shotgun sequence.
ACCESSION   DASTJP010000122 DASTJP010000000
VERSION     DASTJP010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29714"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..229)
                     /gene="secG"
                     /locus_tag="VFQ23_03820"
     CDS             complement(5..229)
                     /gene="secG"
                     /locus_tag="VFQ23_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET9905735.1"
                     /translation="MVFYLNIGLIIISVLLILSVILQSKGAGLGGLTGADAGSVFTAR
                     RGVERTLFWITIVLSIVFFVLVLTLLIIAP"
     gene            437..1516
                     /locus_tag="VFQ23_03825"
     CDS             437..1516
                     /locus_tag="VFQ23_03825"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET9905736.1"
                     /translation="MHEIVVNLHMHTRYSDGSGTHKDIAHAAMKTGLDAVIVTDHNVL
                     VQGVEGYYSLGPSSRIKRNPVLLLVGQEVHDQDRDPQKNHLLIFNANRDLASLADDPQ
                     ALINGVREAGGICFIAHPKDPAAPAFNETDISWEAWDVEGYTGIELWNGLSELKTVVP
                     TKLHGAFYAYFPQFVGHRPIYETLNRWDELLASGRRVVAIGGSDAHAMHMNMGPLHRV
                     IFPYEFHFRTVNTHVFIPQPLTGDVSTDKKMIYDALAAGRCFVGYDLPGSTRGFRFNA
                     KGREQSAIMGDEISVKGGVTLQARLPRAADIHLLKDGKVIGTWKESYAYTHIATEPGV
                     YRIEAYRNYLGSKRGWIFSNPIYVR"
     gene            1631..2152
                     /locus_tag="VFQ23_03830"
     CDS             1631..2152
                     /locus_tag="VFQ23_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905737.1"
                     /translation="MDIHFEKLTQPTPEIAAAFSKWENDPVLIPLTRPNLNEADLLSR
                     KSVTVEDLMDRLKHSHMYLIYAGDELVGEMDYKVDPAYVYKKVAGTAWIGITIGEESG
                     RGKGIGRQALRHLEGQIKSTGLKRIELGVFEFNSQAIKLYQKASYKEIARIEDFTFWQ
                     GRMWQDIRMEKYL"
     gene            2173..2628
                     /locus_tag="VFQ23_03835"
     CDS             2173..2628
                     /locus_tag="VFQ23_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905738.1"
                     /translation="MSFPSQTSNRLDWRAIFEIPLRQFVPWLAAVLLVTFAGYPGVVC
                     VTPMAWLIALRVGNLCAWRSKSTQSTNRLMEAALAGGFFGFLQGLLFAGIITFLEPVN
                     ADEQSRAVILTLIMIVVGMFAGAGLSFFTAFLNEQRRKREQSQPDNMTR"
     gene            complement(2717..3211)
                     /locus_tag="VFQ23_03840"
     CDS             complement(2717..3211)
                     /locus_tag="VFQ23_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905739.1"
                     /translation="MTVVAFILSVIVGTGSLAYGYSQMGLTNPSRWFVLLGILWLVAQ
                     WRKYYWFSSLAIFVIVIAAAFGVWVEFPTIWMLLGALGGLLGWDLSDFARRLSYASSA
                     DDVAGMERRHLARVGIVAVLGFGLALLSLYIRINRLAFEVAVGLVLLAALGLTRLVIG
                     LRRY"
     gene            complement(3354..>3653)
                     /locus_tag="VFQ23_03845"
     CDS             complement(3354..>3653)
                     /locus_tag="VFQ23_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905740.1"
                     /translation="ILISPLHEDDLLPLVQLRAQGYQILIISPNPVKFELSYLPKNRN
                     VELAGRVLRMERDLLLQKIQRASIQVLNWDVAEPFDLVVKRMLSRPPAWLRAVGR"
BASE COUNT         1014 a          961 c          862 g          816 t
ORIGIN      
        1 cagattacgg agcgatgata agcagcgtaa gaaccaacac aaagaataca atgctcaata
       61 caatcgtaat ccagaacaat gtacgctcaa caccacgtcg tgccgtgaac acgcttcccg
      121 catcagctcc ggtcaaccca cccaagcctg ctcccttact ttgcaggatt acactcagga
      181 tgagaaggac cgagattata atcagcccga tattcagata gaaaaccatg tagtatatgc
      241 ctccttatta agcggtgaca ttatacctgc tagggagaaa aaccgtcaac ttggcgcctc
      301 gaatgcttgt gcaaaaacct attaaaaacc cttgttttgc cgcagtgcaa acggagagtc
      361 tgagaaaaaa ctcagagaac tctgtagtct cccctggcac cgcccatcag ggcaggtgtg
      421 tggcaggtct ttaaccatgc acgaaattgt ggtcaattta catatgcata cgcgctactc
      481 agacggctca ggcacccaca aggacatcgc ccatgccgcc atgaaaaccg ggctggacgc
      541 ggtgattgtg acagaccata acgtgttggt tcaaggcgtg gagggctact acagccttgg
      601 tccttcgtct cggatcaaaa ggaatcccgt cctgctcctg gttgggcagg aagtccacga
      661 ccaagatcgt gacccgcaaa aaaatcacct gctgatcttc aacgccaacc gtgacctggc
      721 atctctggcg gatgaccctc aggcccttat caatggcgtg cgtgaggcgg gcggcatttg
      781 cttcatcgcc caccccaaag accctgccgc gcccgccttc aacgagaccg acatctcgtg
      841 ggaagcctgg gacgtggagg gttacaccgg gatcgagttg tggaatggtc ttagtgaact
      901 aaagacagtt gtaccaacca aattacacgg ggcattctat gcgtactttc cacaattcgt
      961 tgggcaccga cccatctatg aaacgttgaa ccgctgggat gaactgcttg ccagtggacg
     1021 gcgcgtagtt gcgattggcg gttcggatgc gcatgccatg cacatgaaca tggggccact
     1081 gcaccgcgtc atctttccgt acgaatttca ctttcgcacg gtcaataccc atgtcttcat
     1141 cccgcagcct ctgacaggag atgtttcgac tgacaaaaag atgatttatg acgccctggc
     1201 cgccggacgc tgttttgtgg gttatgacct gccaggttct acgcgcggct tccgcttcaa
     1261 cgcaaaagga cgtgagcagt ctgcgattat gggagatgaa atttcggtca agggcggcgt
     1321 gacattacaa gcccgcctgc ccagggccgc agatattcat ttgctcaagg atggaaaagt
     1381 catcggaacc tggaaggagt cctatgcata cactcatatc gcgaccgagc cgggagttta
     1441 tcgaattgag gcctatcgca attacctggg aagcaaacgt ggctggatct tcagcaatcc
     1501 aatttatgtg agataacatt cagcagaaag aatttcatcg cgaagtgtgc taaagatgta
     1561 aaggatatat agaaagaaga aagaggaaag aggaaagact cttcgcgtgc tccgcggttc
     1621 aaggaaactc atggacatcc attttgaaaa actgactcaa cccacccccg agatcgctgc
     1681 cgcgttcagc aaatgggaaa atgatccggt gctgatcccg ctcacgcgtc ccaatctaaa
     1741 cgaggcagat ctgttgtcca gaaagtcggt cacagtggaa gatctaatgg atcggcttaa
     1801 acatagccac atgtatttga tttatgcggg agatgagctc gtcggcgaaa tggactataa
     1861 ggtagatccc gcctatgtat ataaaaaggt ggcaggcacc gcctggatcg gaatcacaat
     1921 cggcgaagaa tcggggcgcg gcaaaggaat tggtcgtcag gcgcttcgcc atttggaagg
     1981 acaaatcaaa tcaacaggtt tgaagcgcat tgagttgggt gttttcgaat ttaatagtca
     2041 ggcaattaag ctgtatcaaa aggccagcta caaagagatt gctcgaattg aagactttac
     2101 tttctggcag ggcaggatgt ggcaggatat ccgcatggaa aaatatctgt agaaaagtga
     2161 gaacttaaat caatgtcctt tccatcccaa acctccaata gactggactg gcgcgccatc
     2221 tttgagatcc cgctgcggca gtttgtgccg tggctcgcag ccgttcttct tgtgacattt
     2281 gcgggctatc ccggagttgt ttgtgtcaca ccgatggcat ggctgatcgc attgcgagtt
     2341 ggaaatcttt gcgcctggcg atcaaagagc acgcaatcca caaaccgttt gatggaggcc
     2401 gccctcgccg gtggtttttt tggattttta cagggcctgt tatttgccgg aataatcact
     2461 ttcctggaac cggtcaacgc agatgaacaa tcaagggcgg taattctcac cttgatcatg
     2521 attgtggtag gcatgttcgc tggcgcaggg ctttcctttt ttactgcatt tctaaatgag
     2581 caacgaagaa aacgagagca aagtcagcca gacaacatga ctcgttaaaa attaaaagat
     2641 ttcactctag aggcgttagg accttttgat gaatggacga gtcctctccc cacaaatcta
     2701 aaattctctc gttaaatcaa tacctcctca acccaatcac caaccgcgtc agccccagcg
     2761 ctgccaatag cactaaccca accgcaactt caaatgcgag cctattgatg cggatatata
     2821 gactcaatag cgccaatcca aatcccagca ccgcaacgat acccacacgc gccaaatgac
     2881 gacgttccat tccagccaca tcatcggccg atgaggcata actcaaacgc cgcgcgaaat
     2941 cagagagatc ccatccgagc aagccaccca acgcgccaag cagcatccag attgtgggaa
     3001 attcaaccca cacgccaaat gctgctgcga tgactatgac gaatatagcc aacgaggaaa
     3061 accagtaata ttttctccac tgtgccacaa gccacaggat gcccaatagc acaaaccagc
     3121 gcgacggatt cgttagcccc atctgcgaat acccatacgc cagcgagcct gtccctacga
     3181 taacgctaag aataaatgca acaactgtca atctcaacct cgttcaatta aacaacatga
     3241 ttttactcac agggtggttt cgatacgccc ttcgcaacaa tcgctcaggg ttactcaacc
     3301 accgggacta ctcttaaagc cttagtgtac tatgtatcct tcgtgataaa aaatcatctc
     3361 ccaacggcgc gcagccacgc cggcggacga cttaacatac gcttcaccac aagatcaaac
     3421 ggctctgcca catcccaatt caagacctga atgctggcac gctgtatttt ttgcaataat
     3481 aagtcacgct ccatacggag cactcgccct gccaattcca catttcgatt tttcggcaaa
     3541 taagagagct caaacttgac cgggtttggg ctaatgatca gaatctgata tccctgcgcg
     3601 cgcaattgga ccagcggcag gagatcatct tcatgcaaag gcgaaatcaa aat
//
LOCUS       DASTJP010000123         7449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_435482, whole genome shotgun sequence.
ACCESSION   DASTJP010000123 DASTJP010000000
VERSION     DASTJP010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435482"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1274)
                     /locus_tag="VFQ23_03850"
     CDS             complement(237..1274)
                     /locus_tag="VFQ23_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9905741.1"
                     /translation="MTLHSEIFEQPERLASLLEKQKQSVIEIANAIQAHNVHYAFLAA
                     RGTSDNAGRYANYLWGAHNGLPLALATPSLFTYYQSPPRLQNALVVGVSQSGQSPDIV
                     SVLEEGRRQGCLTLAITNAPDSPLAHVADHVLDIQAGEERAVAATKTYTAELMAIAML
                     SAALSNSDERWNQLANVPMWIEQSLKLDSRIARISERFRYMSQCVVLGRGFNYATAFE
                     WALKLKELTYIIAEPYSSADFQHGPIAMVEGGFPILAVAPTGKVRDSMQEMLKRLRQE
                     QDAELVVISDDTDMLSFAQSPVQLPPHMPEWLTPLVSIIPAQLFACHLTQVKGYDTEK
                     PRSITKVTETH"
     gene            1710..2729
                     /locus_tag="VFQ23_03855"
     CDS             1710..2729
                     /locus_tag="VFQ23_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905742.1"
                     /translation="MYLCVLSSTPDDPNDKPYDPAPHMNGFNWKHHQVNPKNVEKQIR
                     DLMNEGVDVFFNLCDGTPDDALSGIGLVQTMEKLGAAFTGADSKFFDPSRQEMKSYAK
                     KSNVPTPNWAMVDRVEDVERVAKRLRFPVLVKPPHGYASVGITRESRCENIEQLKVQT
                     AREIEQFGRALLEEFIEGREFTCLIAENPDDPKNPITFKPVEFIFPDGESFKHYNMKW
                     VEYEKMSVAPVNNARIEKVLREQTARLFKAMNGNGYARCDYRMGADGTIYMLEINPNC
                     GIFYAPDEPGSADFSLINDPIFNHNKFLKLIIRAAKNRRDNMLASRNAKRQVKKQRVE
                     ELAYT"
     gene            2923..4956
                     /locus_tag="VFQ23_03860"
     CDS             2923..4956
                     /locus_tag="VFQ23_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905743.1"
                     /translation="MEQISARSKVLKNTAWTEIIALVVISVFVYLIHISSFSYYRDDW
                     YYMYDGLVGGSRIFVEMFRNLRPARGPLFEFLFGLFGTNPFPYHLLLYASRVVGGLGA
                     LWLFRILWPKQRAATFFMALLFLIYPGFLWWIQGFEFQPMVLSVGLQIFSIIFTLKAV
                     QANSRATWILWMLASLVSGWAYLAFVEYAIGMEVFRWLCVYLIVRQSAERTHRQTILQ
                     SVRMLLVPMLIPALFLIWRIFLFDNERRAADISLQTGAVFNSPVTLLWWIIHFIQSVL
                     NVSGFAWALPFNGIFYSLRLKDLLFALGWMVIVTVLSYVTYRLTHADAEPAPSRTWQL
                     EAISIGFLGVSAGVVPIIIANRAVTFDRFSHYALPASLAAVMLVVGLIYLMTDTRVRV
                     VSLSILVGLSVLTHHAVAIQAKREEVLINNFWHQIAWRAPALRAGTVLVANYAGLNYA
                     DGADIVWGPANFIYHSETQSQVPIIVPISAARMEADTPKNILEGAQLQQNYIVVNDIS
                     HDYTNLLVLSLPTEDSCVHVLDGQWAELSVRDTALVALAASRSEIENVIIEGEERDLP
                     TTIFGEEPPPTWCYYYQKAQLARQQEDWDAILKFRDEVTKLDLRPNDPIEWMPFLQAA
                     AINGDEKQVKNISTLINTEKLYKQQACQNLMAMNGLSSEIQGYASELFCGGTN"
     gene            complement(4966..6540)
                     /locus_tag="VFQ23_03865"
     CDS             complement(4966..6540)
                     /locus_tag="VFQ23_03865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9905744.1"
                     /translation="MVPNVTNRHFTGISVILLGILLVVLVLNVLVQANPGTHVPGRDY
                     GFYVYIGDQILNGGLPYRDGWESKPPAIFYLNTVGLWIGRGSRWGVWMLEFIALFAAS
                     AISLHTLNKLWGIWPALGGLLLWLWGLHLTLEGGNLTEEYPLTLHFLSIFLFLKLVEV
                     PKHRLYNLLLGLAFGISFLFRPNNAAVEMAVIVTLLLVPVLKRNLTSVFVQVLWLAIG
                     ALAPILLTGLYFWSQGLLRDLLEASLLYNLTYSTTEITAGSPLIKGFITLGWAAWIAV
                     AGYLVGIYRFLKTRDPFYLLLLLGWPLVIYLSDPSKRSYAHYFINWLPFLGMLGGLLV
                     HLVITRVRTAKIHSPASTLMGLTSTLALSLMVFFASGPAAQYQKAVERISERDSIGIE
                     LRTRTAVYVQNHTQPGDLVLFWAAAPGENFMSNRESPSAYLFYPLFVHSEISQRISDQ
                     FLQDIIDKRPILIVDINDHEALSINPQERAEQIAAGFAWEYPPDNLNEFFKFVEENYY
                     LEAKLGDKTVYRLYGQ"
     gene            6684..>7449
                     /gene="proC"
                     /locus_tag="VFQ23_03870"
     CDS             6684..>7449
                     /gene="proC"
                     /locus_tag="VFQ23_03870"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET9905745.1"
                     /translation="MLKNKIVFIGPGVMAEAMIAGLLNKKLANPENITVAGPREERGE
                     QLHNKYGIKSTTDNGTAASHADVVVLSVKPQRLSEVMKGLKGIRSDALVLSIIAGANI
                     KKISTGLKHKSVVRSMPNTPGQIGEGITVWTASKEVTGEQQDIARAMLGALGDEVFVE
                     DESYLDMATALSGSGPAYVFLFTEALIDAGVHMGFPRRIAEQLVLQTIKGSMSYYHYA
                     ERHPATLRNQVTSPGGTSAEALYYLDKAGFRTAISRA"
BASE COUNT         2043 a         1703 c         1786 g         1917 t
ORIGIN      
        1 aaggtcatcc gtttgagttc tatcaaaaga tggggtacat catcacaggt gtcgtgatcg
       61 atgcgaatgg cgttggtaag cccgatatta ttatgtcgaa gagggtagga aaataaaagg
      121 tgatctcgat acagcctcta atcgtatcgg cttactcgac caccggtcga agcaatctcc
      181 tcattaaatt ggagattgcc acgtcggact gaggtcctcc tcgcaacgac aacaggttaa
      241 tgcgtctctg tcactttcgt gatcgagcgc ggcttctccg tatcgtatcc cttgacctga
      301 gtcaggtgac aggcaaataa ctgagcgggg atgatgctta ccagcggtgt cagccattct
      361 ggcatgtgag ggggaagctg aactggggac tgagcgaaag aaagcatatc cgtatcgtca
      421 gaaatcacaa cgagttctgc atcctgctct tggcggaggc gctttagcat ttcctgcatc
      481 gaatctcgta ctttacctgt aggcgctaca gccagaatcg gaaacccacc ctcgaccatc
      541 gcaatgggac cgtgctgaaa atcagccgag gaatacggct ccgcaatgat gtacgtcagt
      601 tctttgagtt tgagcgccca ttcgaaggcg gtcgcataat tgaaaccacg tccaagcaca
      661 acacattggc tcatgtaccg gaatcgctct gagatgcgcg caatacgtga atccagtttc
      721 agggattgtt ctatccacat cggaacattc gccagttgat tccatcgttc gtcagagtta
      781 ctcaacgcgg cggaaagcat ggcaatcgcc atgagttcgg ctgtatacgt cttggtggct
      841 gccaccgccc tttcttcgcc cgcttgaata tcaagtacgt gatcggcaac atgagccagc
      901 ggcgaatcgg gtgcatttgt gattgccagt gttaagcatc cctggcgacg cccttcttct
      961 aacacactga caatatctgg tgattgaccc gattgcgata caccaaccac aagcgcattt
     1021 tgcaaacgtg gaggactctg ataataagta aacagagagg gagttgcaag ggcgagtggt
     1081 aatccattat gagcccccca cagatagttc gcatatcttc cagcattatc tgatgttccc
     1141 cgcgcggcga gaaaggcata gtgcacgttg tgcgcctgaa tggcgttcgc gatctcaatg
     1201 accgactgtt tttgtttctc aagcagagag gcaagtcgct caggttgctc gaatatttct
     1261 gaatgaagtg tcatagtgtc ctcaacattg attctacttc cacttatatc atctttccaa
     1321 gatgaattcg attcgtttca atacacaaat cattttcatt cattcgcttc gtaatgattg
     1381 caccagataa aaatatttca taaacattga ttgatgcata ttaatagaat aacaattgaa
     1441 tcaattcaat gatatttatt gtttatcaaa taaattctaa caaaatttta aagttaatta
     1501 ttagtaaaaa acctttacat aaatcgaata atagatacaa tcaacatcgt tgtataaaat
     1561 aattgcatga ttcaattaat agattcattg aatcaatgat aaagaattaa aacaacaaga
     1621 tgcaatttaa aatacaactc aatcaagaat aaacaacaag atattatgta ttcatcttga
     1681 ttgaatgaaa cgaaagaaaa ggaattgaaa tgtatctctg tgtattgtca agtacgcctg
     1741 atgacccaaa tgataaaccg tatgatccag cgccacatat gaatggattt aactggaaac
     1801 atcatcaggt aaacccaaag aacgttgaaa aacaaattcg cgatctcatg aatgaaggag
     1861 tcgatgtatt ctttaatctc tgtgatggca cacccgatga tgcactttct ggaatcggac
     1921 ttgtgcagac catggaaaaa cttggcgcag catttaccgg tgcggattca aagttctttg
     1981 atccctcacg ccaggaaatg aaatcctatg ccaaaaaatc aaatgtccct acgccaaatt
     2041 gggcgatggt ggatcgggta gaagatgtag aacgcgttgc caagcgactg cgcttccctg
     2101 tgctggtcaa acccccgcat ggatatgcaa gtgtgggcat tacgcgcgag tcacgctgtg
     2161 agaatatcga acagctcaaa gtgcaaacgg cccgtgaaat cgagcaattt ggacgtgctc
     2221 tcctcgaaga gttcatcgaa ggacgcgaat ttacctgcct catcgcggaa aatccggatg
     2281 acccgaagaa tccgatcacg ttcaaacctg tggaatttat tttccccgat ggtgagtcat
     2341 tcaagcacta caatatgaag tgggttgagt atgagaagat gtctgtggct cccgtgaata
     2401 acgcgcgcat tgaaaaagtg ttgcgcgaac agactgcccg tttattcaaa gcgatgaatg
     2461 ggaatggcta tgcacgctgt gactatcgca tgggtgcaga tggcacaatc tatatgctcg
     2521 agatcaaccc gaactgtggt attttctatg ctccggacga accgggttca gcagactttt
     2581 cgttgatcaa tgacccgatc tttaaccata ataagtttct taagttgatc atccgtgccg
     2641 cgaaaaaccg acgagataac atgctcgcca gcagaaacgc caagcgacag gtcaagaagc
     2701 aacgcgtgga agaactggct tacacttaat ataacttcaa ccgatatctt gtaattgata
     2761 agcggacact atgtgtccgc ttatttattg tctcgataaa atggtcatga tgtgataagg
     2821 ctttagaggg gaatttcatg ttctgtagga aacccattat cgaataaatt tctcaataac
     2881 ttataattgt gcctctggag gtcaccaact gtagaagcac gaatggaaca aatatctgca
     2941 cgatcaaaag ttcttaagaa cactgcctgg acggaaatca ttgcgctagt ggtcatttcc
     3001 gtgtttgtct atttaattca cattagcagt ttctcgtatt atcgcgacga ttggtactat
     3061 atgtatgatg gattggtcgg cgggagccgc atctttgtgg aaatgttccg aaatttgcgt
     3121 ccggcgcgcg gcccactttt tgaattttta tttggcttat ttggaacaaa cccgttccct
     3181 tatcaccttt tgctgtatgc atcacgcgtc gtcggaggtt tgggtgcgct atggcttttc
     3241 cgtatccttt ggcccaaaca gcgagctgcc acctttttta tggctctgct ttttttgatc
     3301 taccccgggt ttctatggtg gatccagggt tttgaatttc agccaatggt gttaagtgtc
     3361 ggactgcaga tcttttcaat catttttaca ttaaaagcgg tccaggcgaa ttcacgcgca
     3421 acctggatac tgtggatgct ggcatcttta gtatcgggct gggcctatct tgctttcgtg
     3481 gagtacgcca ttggcatgga agtcttccga tggctgtgtg tgtatctcat cgtcagacaa
     3541 tccgcggaaa gaacgcacag gcaaacaata cttcaaagcg tgcggatgct gctcgtacca
     3601 atgctcatcc ctgccctgtt tctcatttgg cggatcttcc ttttcgataa cgaacgtaga
     3661 gctgcagaca taagtttgca aaccggagct gtattcaatt caccagtcac tctattatgg
     3721 tggatcatcc acttcattca gagtgttctg aacgtatctg gttttgcctg ggcgctgccg
     3781 ttcaacggaa ttttttactc tcttcgtcta aaggacttat tatttgccct tggctggatg
     3841 gtgattgtta cagtactctc ctatgtgacg tatcggctga ctcatgcgga tgctgaaccc
     3901 gctccctcgc ggacctggca actggaggca atttcgattg gtttcctggg cgtcagtgca
     3961 ggtgtggttc cgattatcat cgccaatcgc gccgtcacct tcgaccgttt ctcgcattat
     4021 gctctgccag catctctggc agcagtgatg ttagttgtag gtttgattta tttgatgaca
     4081 gatacgcgag tacgcgttgt gagcctgtcg attctagtgg gattgtctgt tttgacccat
     4141 catgcggtag ccattcaggc aaagcgcgaa gaggtgctca tcaacaactt ctggcaccaa
     4201 attgcctggc gcgccccggc gttgcgcgca ggaaccgtac tagttgcgaa ttatgcaggc
     4261 ctgaactatg ccgatggcgc cgatattgtc tggggaccag ctaatttcat ttatcattcg
     4321 gaaactcaaa gtcaggtgcc gatcatagtc cccatttctg cggcacggat ggaagctgac
     4381 acgccgaaaa atattctgga aggtgcgcag ttacagcaaa actacatcgt ggtaaatgac
     4441 atttcacatg attacaccaa tttgttagtt ctgagtctgc ccactgagga ttcctgtgtc
     4501 catgtgctgg atgggcagtg ggccgaattg tcggttcgcg atacggcttt ggtcgcgtta
     4561 gccgcctccc gctcggagat cgaaaatgtc atcatagaag gcgaagaacg ggatttgcct
     4621 acaactattt tcggcgaaga accacctcct acatggtgtt attactacca gaaggcacaa
     4681 ttagcccgac agcaggaaga ttgggatgcg atcttgaaat ttcgggacga agtgacaaaa
     4741 cttgatctgc gtcccaacga cccaattgaa tggatgccgt tcctacaggc tgcggccata
     4801 aatggggatg aaaaacaagt gaagaacatt tccacactga tcaacacaga gaagttatat
     4861 aaacagcaag cctgccaaaa tctgatggca atgaatggat taagctccga aattcaaggt
     4921 tacgcaagtg aattgttttg cggcggtacg aattgatatt tggttttact gtccatacag
     4981 ccgatatacc gtcttatccc cgagcttcgc ttcgagataa taattctctt ccacaaattt
     5041 aaaaaactca ttcaaattgt caggagggta ttcccaggcg aaacctgctg caatttgttc
     5101 tgcacgttct tgcgggttaa tggacagggc ttcatgatcg ttgatatcaa caattaggat
     5161 gggtcgttta tcaatgatat cttgtaagaa ctgatcgctg atccgctgtg aaatctctga
     5221 gtgaacaaag agtggataga ataaatacgc actgggcgat tcgcgatttg acatgaagtt
     5281 ttcgccgggc gcagcagccc aaaataaaac aagatcaccg ggctgtgtat ggttttgtac
     5341 atacacagcg gtacgagtgc gaagctcgat cccgatgctg tcacgttcag aaatccgctc
     5401 cacggctttc tggtattgag cggcgggacc actggcgaaa aagaccatca ggctaagcgc
     5461 cagggtggaa gttaacccca tcaatgtaga ggccggagaa tgaatttttg cagttcgcac
     5521 acgagtgatc acgagatgga ccagcaaccc acccaacatg ccaagaaagg ggagccagtt
     5581 gatgaaatag tgagcatagc ttcttttgga tggatcgctg agataaataa ccagcggcca
     5641 accaagcagg agcagtaaat aaaacgggtc tctggttttt aaaaagcgat aaattcccac
     5701 cagataccct gcaaccgcaa tccacgccgc ccagcctaat gtgatgaaac ccttgatcag
     5761 aggagaacca gctgtgattt cagtcgtgct gtacgtcagg ttgtagagga gggaggcttc
     5821 gagtaggtcg cgcaacaacc cctgggacca aaaataaagg cctgtaagta atatcggggc
     5881 gagtgctccg atagcaagcc aaagcacctg tacaaaaaca gaggttaaat ttctcttcaa
     5941 gaccgggaca agcaataaag taacaatgac agccatttcg accgcggcat tgttcggccg
     6001 aaagagaaaa ctgattccaa acgccagccc cagaagaagg ttgtatagcc ggtgctttgg
     6061 cacctcgacc agtttcagaa acaggaagat ggacaaaaaa tgcagcgtca atggatattc
     6121 ttctgtaagg tttcctcctt ccagggtgag atgtaagccc caaagccaga gaagcaagcc
     6181 tcccaatgcg ggccaaatgc cccagagctt gttcagcgta tgaagagaga tcgcactcgc
     6241 ggcgaataat gcgatgaatt caagcatcca tacgccccag cgcgaacctc gccctatcca
     6301 caagccgacc gtattgaggt aaaagatcgc agggggcttg ctttcccaac catctcgata
     6361 gggaagccct ccgtttagaa tttgatctcc aatataaacg tagaaaccat aatcccgacc
     6421 cggaacgtga gtcccgggat tggcttgaac caatacattc agtacgagca caaccagcag
     6481 gatgccaagt aaaatgactg agatgcctgt gaaatgtcgg ttagttacat ttgggaccat
     6541 atatctcaca tgtcagtaag atgtgcacat gataactttg gaaggcaacg aatccacata
     6601 aaatagtgat ttattgtact ctcacaagat acgggcttga gtaaatctgt ccgttctttg
     6661 tataatgtct tctcggagga ataatgctaa aaaataaaat tgtttttatt ggtcccggtg
     6721 taatggcgga agcgatgatc gcaggtcttt tgaataagaa attggcaaac cctgaaaata
     6781 ttacggttgc aggtccgcgc gaagaacgcg gcgagcagct gcataacaaa tacgggataa
     6841 aatccacgac cgataacgga acggccgcct cccatgcgga tgtcgttgtt ctttcagtca
     6901 agccacagcg cctcagcgaa gtgatgaaag ggctcaaagg cattcgcagc gatgcattgg
     6961 tattgtccat aattgcgggt gcaaatatca agaagatcag cactgggtta aagcataaat
     7021 cggtggttcg ctcgatgccc aatacgccgg ggcagatcgg cgagggaatt acagtctgga
     7081 cagcttcgaa agaagtgacc ggcgaacaac aggatatcgc ccgcgccatg ctgggcgcgc
     7141 tcggcgatga agttttcgtg gaagacgaga gttatctgga tatggctact gcattatctg
     7201 gttcaggtcc cgcgtatgtc tttttgttta ccgaagcact gatcgacgca ggggtgcata
     7261 tgggctttcc acgccgcatt gcggagcagt tggtcttaca gaccatcaaa ggttctatgt
     7321 cttattatca ctacgcggaa cgacatcctg ccaccttgcg gaaccaggtc acatcgcccg
     7381 gtggaacgtc tgccgaagcg ctctattatc tggataaggc aggtttccgc acagccattt
     7441 cacgagccg
//
LOCUS       DASTJP010000124         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_836749, whole genome shotgun sequence.
ACCESSION   DASTJP010000124 DASTJP010000000
VERSION     DASTJP010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836749"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VFQ23_03875"
     CDS             complement(<1..874)
                     /locus_tag="VFQ23_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9905746.1"
                     /translation="MSLLLIMLLILTTMVLLLMFYGPKPDLNTLHIIDETLNEELPEL
                     VRGKTGFAVSNNVGIWYELIPPTTTSIKGTVCLLMSNAADALMWPPSFINQFTSAGFQ
                     VIRLDYRGTGLSDWMSNWNKNNPYSLFDMAKDVISVIDTLHISRVHLFGFSFGGMIAQ
                     EIAINNPERISSLTLMMTTGDATDPKMKTMSTSYVFSSIIKSIPIIKYRILGGERNLI
                     IERILKMQAGQMNFDVKETAQQVLYDLRNRKGINFKAVFQHFKAVEISGSRYEKLKDI
                     KTPTLIIHGTKDNIF"
     gene            1377..1538
                     /locus_tag="VFQ23_03880"
     CDS             1377..1538
                     /locus_tag="VFQ23_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905747.1"
                     /translation="MPPVEVRELRALISYRWRLVKMSTAIQNRLHSLLHRHNIQAPVG
                     KVDTGENRA"
     gene            1548..1814
                     /locus_tag="VFQ23_03885"
     CDS             1548..1814
                     /locus_tag="VFQ23_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9905748.1"
                     /translation="MKLSELETLRLKQELTSLRMVRDHIAEVETELGRLSTSERWGKQ
                     AVYLMQLPGVGVVVTMTILAAIGDIRRFESAKKDVLPPPKKSSP"
BASE COUNT          546 a          435 c          482 g          591 t
ORIGIN      
        1 ggaatatatt atctttcgtc ccatgtatga taagtgttgg tgtttttatg tctttcaatt
       61 tttcatatct cgatccagat atttcaacag ctttaaaatg ctgaaatact gccttaaaat
      121 taatcccttt cctgtttctc aaatcatata atacctgttg tgctgtctct ttgacatcaa
      181 aattcatttg tccggcttgc atttttagta ttcgttcaat tattaaattc ctttcacctc
      241 caagaatacg atattttatg attggaatag attttattat cgaactaaaa acgtagctcg
      301 tggacattgt tttcattttg ggatccgtgg catcacctgt tgtcatcatt aaagtaagag
      361 aggaaattct ttccggattg ttaattgcta tttcttgagc aatcatacct ccgaaagaaa
      421 atccaaaaag gtgaacacgg ctaatatgca atgtatcaat gacggatatg acatcctttg
      481 ccatatcaaa aagcgagtat ggattatttt tattccaatt actcatccaa tctgataatc
      541 cagtacctcg atagtctaat ctgatcactt gaaatcctgc agatgtaaac tgattaataa
      601 atgaaggtgg ccacattaaa gcgtcagctg catttgacat caataaacat accgtgcctt
      661 ttatggaggt tgtggttgga ggaattaact cataccagat tcccacatta tttgaaactg
      721 caaaaccagt ttttcctctt actaattcag gtaactcctc atttaatgtt tcatcaataa
      781 tatgtaaagt attcaaatca ggtttggggc cataaaacat taataatagc accattgtag
      841 tgagaattaa taacataatc agcaataggg acatgctaag tactccttag tcgctctaac
      901 ggctcgcgtg cggcttggtg ggataaccaa cgaaatgaaa aaatgctcat ggccgagacg
      961 gcgatttttt cgcggcgcgt accaccaagt ctgcctgcat gccttgttat tcgaatgagt
     1021 tgtgttgagc gtgggaggta ctatccttgg aaggatggag cgccggggac atgtctgcgg
     1081 caggagcatc agggtcagcc ggttgcggct ttgagatgtt caggtaggtt gtgaacaagc
     1141 ccgtcgtgtt atttgactgc ccggcggatt tcaaatctgg gactccctgg acaagaggga
     1201 agaacctgta ttggtaggtg taactcgtga cccaccaatc cacgcctcaa cttcaagcag
     1261 gaggagtgta gcatgaaaag cgagaggcag acaagagagg gaccctatat tggtttcgat
     1321 gtccacaagc agtacatcgt attaagagcg cgtgatgtgg tcatggaacg gtttgggtgc
     1381 caccggtaga agtgcgggaa ttacgagccc tgatctcgta tcgttggcga ttggtgaaga
     1441 tgagtactgc gatccagaac cgtctgcaca gtcttttgca tcggcacaat atccaggcac
     1501 cggtgggcaa agtggataca ggcgagaacc gggcatagtg ggagcaagtg aagctgagtg
     1561 agttagagac tctgcgtttg aagcaggaat tgacgtcctt gcggatggtg cgcgaccata
     1621 ttgcggaagt ggaaacagaa cttggacggc taagtaccag tgaacgttgg ggaaaacagg
     1681 cagtgtatct gatgcagctt cccggtgtgg gagttgtggt caccatgacg atcttagctg
     1741 ccattggaga cattcgcaga tttgagagtg ctaagaaaga tgtattgccc ccaccgaaga
     1801 agtcctcgcc ttgagccctc ctccctcttg acactgctac ttatcggtag gcagcttttt
     1861 agtatcccac tcacctaaga gattcggtgg agataatctc agggtattgc ctatctactg
     1921 caccgcctct ctgatctggc gttccatctc gggcgttatt tctgtggatc gagcgggaag
     1981 ccccgtcgtc cctcgttctc cattctgctc aatatcctcg ataagcagct tcatctcggc
     2041 tatttcgcgg acct
//
LOCUS       DASTJP010000125         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_271117, whole genome shotgun sequence.
ACCESSION   DASTJP010000125 DASTJP010000000
VERSION     DASTJP010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271117"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1550
                     /locus_tag="VFQ23_03890"
     CDS             147..1550
                     /locus_tag="VFQ23_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9905749.1"
                     /translation="MPLTLSNFTQLENFGHSLRAPSYRFRPTRTEEICEVFRLAEASG
                     LSVTARGAGRSYNDAALNGGGIMLDLSAMDQILEWDPATGLVRCEPGVTLEKLWQRVE
                     PEGWWPPVVSGTMKTTLGGCLAANIHGKNNFKMGTIGEHVLEFTALLPTGAEITCTPK
                     ENPDLFHGMISGLGMLGVFTSITMQMKRIESGLLEVHAWPVHDLHEHLSFLLEHAPHY
                     DYIVGWMDSMNGGKSLGRGQIHAANYLHGNEDPNPQQTMQLSNQVLPPRIFGVFPKSI
                     LYLFMAPFANNLGWWGVSTAKYIASLRKHTFRQSHAAFHFLLDYVPNWELSYGRGGLI
                     QYQSFLPKETAEDTWREMIELSLKLGLPSYLGVTKRHRPDKFLLTHAVDGFSLALDFK
                     VTDGNRSALSKMLQEFDRMVLKAGGRFYFAKNSETSAEAARAFLGEETIQKFKDLKKR
                     CDPNNLLESDLFRRVFE"
     gene            1543..2967
                     /gene="sthA"
                     /locus_tag="VFQ23_03895"
     CDS             1543..2967
                     /gene="sthA"
                     /locus_tag="VFQ23_03895"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HET9905750.1"
                     /translation="MNKGNLTEKFDLIVIGAGPAGEKGAAKAAQYGKRVALIEREPYL
                     GGAGINTGTVPSKTLRESALYFSGLRQRGLYGIDYSLKDNLTIKDFMYRERKVVRKER
                     RMIAEHIDEHNISLLHGEGSLKDKNTVLVRSSKGEQEVSGDFILIATGSSPHHPPEIP
                     FDGELVFDSDSILNMKQIPGKMVVVGGGVIGTEYASIFMALGIRVTLIEPKGRIVSFV
                     DSEIGQRLMDQLLKLGIDFIFDDRMTAIEARKEHVQLTLEKGGKREFDVALIAAGRQS
                     NVGGLGLEEVGVKLGNRGLILVDENFRTNIPNIYAVGDVIGFPALASTSMEQARAAVV
                     DAFDLKYKEHLAPFLPLAVYAIPEISSVGLTEDDCKTKSIPYLVGRAYYEENARGQII
                     GDMSGMIKLVFSPTDKKLLGAHIIGEQASELIHVATHVMLKGGPIDEFIDAVYNYPTL
                     SDSYQYAAYDGLANHAKWLETNKK"
     gene            2964..>3279
                     /locus_tag="VFQ23_03900"
     CDS             2964..>3279
                     /locus_tag="VFQ23_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905751.1"
                     /translation="MIVEVLDLGLIEYERAWKLQDEYAREIAEGKRRPTVLLLEHPHV
                     YTFGRRGRQENLLWGESQLREKGIAIHWVDRGGDVTYHGPGQLVGYPLIPLGAQSLQT
                     LQS"
BASE COUNT          789 a          804 c          911 g          775 t
ORIGIN      
        1 aacgcaaaca ggtgatttac accgcctcca tctggcgctg gatcatgctt gcgatccagc
       61 atacaccgtc aattattttc cgcaggttgt cattctaaaa ccatccaaac gccatcactg
      121 gagaattcat tcacatggaa acgaacatgc ctctcacatt gagcaacttc acacaacttg
      181 aaaactttgg ccactcctta cgtgcgccat cctatcgatt tcgtcccact cgcacggaag
      241 agatttgcga agttttccgt cttgcagaag cgtcagggct ttctgtcacg gcacgcggcg
      301 ccggaagaag ctacaacgac gcggcgctga acggcggcgg catcatgctg gacctctcag
      361 cgatggacca aatccttgag tgggatcctg ctacgggcct ggtgcgatgc gaacctggtg
      421 tcacactgga aaagttatgg cagcgcgtcg agccggaagg ctggtggcct ccggtcgtct
      481 ccgggacaat gaagaccacg ctggggggat gtctcgccgc gaacatccac ggtaagaata
      541 atttcaagat gggaaccatc ggcgagcatg tcctcgagtt cactgcatta ctgcctacag
      601 gtgcagagat cacctgtact ccgaaagaga accctgacct gttccacggc atgatcagcg
      661 gactcggcat gttgggtgtt ttcacttcaa ttaccatgca gatgaaacgt atcgagtccg
      721 ggctcctgga agtgcatgcc tggccggtgc atgatctgca tgaacatctt tctttcctgc
      781 tcgagcatgc tcctcactat gattacattg tgggttggat ggattcgatg aatggtggaa
      841 agagcctggg ccggggccag atccatgctg ccaattattt gcatggcaac gaagacccga
      901 acccgcagca aacgatgcag ttgagtaatc aggtgctgcc gccgcgcatc tttggcgtat
      961 ttcccaagtc cattttgtat ttatttatgg ccccattcgc gaacaatctg ggctggtggg
     1021 gagtaagcac ggcgaagtat attgcatcgc tccgcaagca tacgttccgc caatctcatg
     1081 cagcctttca tttcctcctg gattacgtcc ccaattggga attgtcgtat ggccgcggtg
     1141 ggctcattca gtatcaatcc ttcctgccca aagagaccgc cgaggatacc tggcgtgaga
     1201 tgatagagct ttctctgaaa cttggacttc cctcgtattt gggcgtgacc aagcgtcacc
     1261 gtccagataa attcctgctc acccatgctg tggacggttt ttcactggca ttggatttca
     1321 aagtgacaga tggaaatcgt tctgcgttaa gcaagatgtt acaagagttt gaccgcatgg
     1381 tcttgaaagc gggtgggcgc ttctactttg ccaagaatag cgaaacttca gccgaagcgg
     1441 cgcgcgcttt tctgggcgag gaaacaattc aaaagttcaa agatcttaag aagcgctgcg
     1501 atccgaataa tttattggaa tccgatttat ttaggagagt gtttgaataa agggaatttg
     1561 acggaaaaat ttgatctgat cgttattggt gccgggccag cgggagaaaa aggtgcagca
     1621 aaagccgctc aatatggcaa gcgagtcgcg ctgatcgagc gcgagccata tctgggcggc
     1681 gccgggatta ataccgggac cgtcccatca aagacactgc gtgagtctgc cctttatttt
     1741 tcggggctcc gtcagcgcgg cctgtacggg atcgattatt cactcaaaga taacttaaca
     1801 atcaaagact tcatgtaccg cgagcgaaag gtggtaagaa aagaacgcag gatgatcgcg
     1861 gaacatatcg atgagcataa tatttctctc cttcatgggg aaggctccct caaggacaaa
     1921 aatacggtcc tggtcaggtc ttcaaagggc gaacaagaag tttccggtga cttcatcttg
     1981 atcgccacag gctcctcgcc gcaccatcca ccggaaatcc cgtttgacgg tgagctggtc
     2041 ttcgactcgg attcgatcct gaatatgaag caaatccccg ggaagatggt ggtcgtgggt
     2101 ggaggcgtga tcggtacgga atatgcttct attttcatgg cgctgggcat ccgcgtgacg
     2161 ctgatcgagc cgaagggacg cattgtttct ttcgtcgact cggagatcgg tcagcgcctg
     2221 atggatcagc tcttgaaact tggcattgac tttatcttcg atgaccgcat gactgcgatt
     2281 gaagcccgta aagaacatgt tcaactgacg ctggaaaagg gcggcaagcg cgagtttgat
     2341 gttgcgttga tcgcagcagg cagacaaagc aatgtgggag ggttgggact ggaggaggtt
     2401 ggagtaaagt tgggcaaccg cgggttgatc ctcgtggatg aaaactttcg gacgaatatt
     2461 ccaaatatct atgcggtggg agatgtgatc ggttttcctg ccctggcttc cacctcgatg
     2521 gagcaggccc gcgctgcggt cgtcgatgcc ttcgacctga agtacaagga acatcttgca
     2581 ccatttctgc ctttagcagt ctacgcaatc ccggagattt cctcggtcgg tctcacggaa
     2641 gatgactgta agacaaaaag cattccctat cttgttggac gagcctatta tgaggaaaac
     2701 gcgcgcggac agatcatcgg tgatatgagc gggatgatca agcttgtctt ttcgccgacg
     2761 gataaaaagc ttctgggcgc gcatattatc ggggaacagg cttccgaact gatccacgtt
     2821 gccacgcatg tcatgctgaa gggcggcccc atcgatgagt tcatcgatgc ggtgtacaac
     2881 tatcctacat tgtcggattc ctatcagtac gctgcgtatg acggactggc aaaccacgcg
     2941 aagtggcttg aaaccaacaa aaaatgattg tcgaagttct tgacctgggg ttgatcgagt
     3001 atgagcgtgc ctggaagttg caggacgagt acgcgcgtga aattgcagag gggaaacgcc
     3061 ggccgactgt attgctgctg gaacatcctc acgtctatac gttcggtcgg cgagggaggc
     3121 aggaaaacct gctttggggc gagtcgcaac tcagggaaaa agggatcgcc atccactggg
     3181 tggatcgggg aggggatgtc acgtatcatg gaccggggca gctggtggga tatccgctca
     3241 ttccattagg agcgcaaagt ctccagactt tgcagagcc
//
LOCUS       DASTJP010000126         8977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_662781, whole genome shotgun sequence.
ACCESSION   DASTJP010000126 DASTJP010000000
VERSION     DASTJP010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662781"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFQ23_03905"
     CDS             complement(<1..671)
                     /locus_tag="VFQ23_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9905752.1"
                     /translation="MRKVFKWIGIVLGSLVGLILIAAVTLFLMGNARLNKTYEFPATN
                     LEIPTDAESLAYGKYRVETMCAGCHGPDLSGIENWFDGGPLGTIDSANLTTGEGGLGD
                     DFSTEDFVRAVRHGIDPDGKPIFMIAVGATSHLSDDDLASIIAYIRTVPPVDHVVAGK
                     QFTPLAKMLFAAGILPTMPVEEVTHEVHVSAPERGASAAYGEYAVNINDCRICHGPEL
                     NGGPFP"
     gene            827..3814
                     /locus_tag="VFQ23_03910"
     CDS             827..3814
                     /locus_tag="VFQ23_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET9905753.1"
                     /translation="MAELFFSLLGRLQIVHRQSGTITFTKRKAIGLLAYLLIESDHAH
                     SREFLLGLLWPDLPTASAQNNLRVTWAHLQKALGTNASDEQPFLIGDRLAVRFNPLSD
                     HELDVTRFNSLTEASRLHPHADPHTCGECAARLAQALELVRGELLEGFSLDDCEQFDD
                     WLRIQREQFRLQVIRALEQLGAFHEHSGQLAEAEHAIRQLLEYDPLRESAYRQLMSVL
                     ARADRRSAALDVYETCRRVLGAQLGVALSVETITLAEQIRALAPVKSHRAQSFLPPGL
                     TRFFGRQPESARLVDFLSRRTVRLVTLAGPGGVGKTRLALEVAHCMAGVFAHDICFVE
                     LAGVTDEKSVDDAVAAALHLPTSTGRSSTAAIVDYLHDKTMLLVLDNCEHLVKACARL
                     VQALCQAAEGLTVLATSRIPLHIDEEHVVRLEPFATPTINEAKRLTVADALNFDSVQL
                     FTDRAAQSLLQFRLTDANVAAVVRICQHLDGIPLAIEIAAAQTRALPIEALAERLSQR
                     FAWLNRQVSETLPRQRTLHALIDWSYELLSPQARSALRSLSVFAGGWTLEAAEAVTAS
                     GAACAEILTELVDHSLVKFGMDAGRQRYTMHETIRQFAQAQLRTAKQKSNVLERHARY
                     YAGFVSHAAENRTGQPFPERLRTVVNDHDNLRQGFEWLATRDREQALAFVAQLGTKLN
                     FWELGGFFQEGRRWLQRALEGTEDSVSIERARALLAAAELSSAITDFELGLHCVQQAQ
                     HLFQQLGDQQGMIDARLTYCNLAGLAGKDTKIQAQAEEALRMAEQLSYTFGIARARRI
                     LGSIAYDADMPETALQHHLPSVALWRELENPFELANAINNLGVVLMEIGEYVEARQAF
                     EETREIFHSLGYQRGVALAMHNLGETAYKMGEYGNAREHLSESLRIRQHLGLPRGYPY
                     SFELLAQVNDKEGRYQKAVQLLAASEALRVRIGAPLEQVAQKHVTAVLASARHHLGDV
                     AYELAWSKGAAMTTEQAITLALS"
     gene            complement(3891..4292)
                     /locus_tag="VFQ23_03915"
     CDS             complement(3891..4292)
                     /locus_tag="VFQ23_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HET9905754.1"
                     /translation="MEIPRHWRLKKQRYGLVGEVCPHCDYKIFPPRDVCPNCGDEAKD
                     LYTFSGKGEVYSYTTVYEAPSGYDANAPYTVALIKLDEGPMITAQLTDVDNNAVEIGM
                     PVEMVTRKMRNDGDERGLIVYGYKFRPAVAA"
     gene            complement(4533..5021)
                     /locus_tag="VFQ23_03920"
     CDS             complement(4533..5021)
                     /locus_tag="VFQ23_03920"
                     /inference="COORDINATES: protein motif:HMM:NF023621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HET9905755.1"
                     /translation="MGLFSKILEKLGLKKDKDAQGGATATVSQKPAAAPAPKATAPAK
                     PAPTTTSRPASTPKPAPVPVTVPKAPVATPISEVDVVSRLEDLAARNPAKLNWKTSIV
                     DLLKLLDLESSFEDRKEMAIELGCPPELMNDSAKMNTWLHKKVLQEVARNGGNVPKEL
                     LD"
     gene            5259..6479
                     /locus_tag="VFQ23_03925"
     CDS             5259..6479
                     /locus_tag="VFQ23_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9905756.1"
                     /translation="MKILLLNQAFVSPEEPGHTRHFEMAQYLLKHGHELVIVASDLNY
                     QTGQRIVKHRGVFTEQFTDGVRILRSYIYPELHRSYFWRIISFFSFMFSSVWTALFVK
                     DPDIIMGTTPPIFQALSAWFVAFLRRKPFLLEVRDLWPEFGISMGVITNPLVIAPARW
                     LENFLYRRATHILVNSPAYKEYLLGKGVPEKKITFIPYGTDIEMFNPSIDGAHIRKKY
                     GLESKFTVLYAGALGQANDIDTIVRAATRLQAESHIRFVLFGDGKERTRLEVEVRNRA
                     LDNIIFAGAVPKKEMPSVLASSDVCLAILQNIPMFRTTYPNKVFDYMAAGRATVLVID
                     GVIREVIESSNGGIYVNPGDDQLLAKTILELSKDPERTNQLGENARAYLVKHLDRRDK
                     LNDTLHLLQNLVNV"
     gene            6476..7345
                     /locus_tag="VFQ23_03930"
     CDS             6476..7345
                     /locus_tag="VFQ23_03930"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9905757.1"
                     /translation="MKVFVTGATGFTGSRVVPLLLRRGYQVRCLARTTSDRSPLPQPE
                     IEWVQGDLSDAAALTASMQGTDALVNVASLGFGHAESIVSAAKNAGIQRAVFISTTAI
                     FTQLSAKSKKVRLAAEHAIESSGLNYTILRPTMIYGSPRDRNMWRLIRLMRYSPIIPV
                     FGDGNYLQQPVYAGDVAQATVNVIGNEQTLCKSYNIAGKHPLTYNEVIETIARQMKRR
                     VIKLHLPSQPVVALLRLFERIRIPLPLKSEQVLRLNEDKAFSYTDAEKDFDYSPLAFE
                     DGIKLELEYLRGH"
     gene            7347..8354
                     /locus_tag="VFQ23_03935"
     CDS             7347..8354
                     /locus_tag="VFQ23_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905758.1"
                     /translation="MLRVAVFCLLVAISYVGVFYIRRLALRHKIMDLPNEGSSHSKPM
                     PLGGGLVIAILVLAVGAWVANEIDWKRSLIYIASGSVLAWMGWHDDVYPLPARFRLLV
                     QGMVAAISIWGMGYFKVLTIASAGESQLGLFGILITFLWIVGLINAFNFMDGIDGMAG
                     GVAFSAGLGWIWLSLNVNNMFAFWVALAIAASSLGFLVHNWPPAKIFMGDVASTFLGY
                     TFAVLSLIVSDQRGDALLIGTLLMWTVIMDAGVTFLARLFKRENVLSRHRSHLFQRLV
                     ISGYKHSTISSLYIVLTLLAGYLSHIWLLGSYIAPPLIFAGLVLIWSLLTFHAARLQN
                     P"
     gene            8452..>8977
                     /locus_tag="VFQ23_03940"
     CDS             8452..>8977
                     /locus_tag="VFQ23_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905759.1"
                     /translation="MRPFLVLLSLSAYCLSSCNASKRIRIECPNIETNLSSLKSSGSL
                     ILDGDGTYLRNMDSLATTKLNKPDEGLYYFAFSPKRDWISYVSSLTDDLLIANVESEV
                     IKAILWEDDWAYANWLNEEQLIISLIQKDQTSNSSNFLVLNPFTNERNLLIAEFPQIY
                     YMQPWRIIEYSAQID"
BASE COUNT         2068 a         2246 c         2322 g         2341 t
ORIGIN      
        1 gggaacggac cgccattcag ttccggtcca tgacagatgc ggcaatcgtt gatattcacc
       61 gcatattcgc catatgcggc acttgctccc cgttcgggtg cgctgacatg cacttcgtgg
      121 gtcacctcct ccaccggcat agtgggcaga atgcctgcgg caaacaacat ctttgccagc
      181 ggtgtgaact gttttccagc caccacgtgg tccaccggcg ggacggttcg gatgtaggca
      241 atgatcgagg caaggtcgtc gtcgcttaaa tgagatgtcg ctcccaccgc gatcataaag
      301 atgggtttcc catccgggtc gatgccgtgc cgaaccgcgc gcacaaaatc ttcggtggaa
      361 aaatcatcgc cgagcccacc ttcaccagtc gttaagttgg cagaatcgat tgtccccagt
      421 ggaccgccgt cgaaccaatt ttcgattcca ctcaaatccg ggccgtgaca accagcacac
      481 atcgtctcca ctcgatattt gccgtaggcg aggctttcag catccgttgg aatttcaagg
      541 ttcgttgcag gaaattcata tgtcttattg aggcgggcgt tgcccatcaa aaataaagtt
      601 actgccgcaa tgagaattag acctaccaac gaaccaagca cgattccgat ccatttaaaa
      661 actttcctca tattaagagg actcctttgg tcaacgtgag gcaatcagcg taaagatgtc
      721 aaatgccttc atgaaataag aatcatctac tcattacttt cgtttgccat gctatacttt
      781 tgcatcatag agaaacgcca ttaattcatc attatctgag acgaaattgg ctgaactgtt
      841 tttttccctc ctcggacgtt tgcagattgt gcatcgtcag tccggaacca tcaccttcac
      901 caaacgcaaa gcgattggat tgctggctta tcttctgatc gagtcagatc acgcgcacag
      961 ccgtgaattc ctgctgggct tgctgtggcc cgacctgccc accgcttccg cacaaaacaa
     1021 tctgcgcgtc acgtgggcgc atcttcagaa agccctggga acgaacgctt ccgatgaaca
     1081 gcccttcctg atcggtgacc ggctcgcggt gcgtttcaat cctctcagcg atcacgaact
     1141 ggatgtgacc cgcttcaact ccctgaccga ggcctctcgc ctccaccccc acgctgatcc
     1201 acacacttgt ggtgagtgcg ccgcccggct ggctcaggcg ctcgaattgg tgcgcggcga
     1261 gcttttggaa ggattttcgc tcgacgattg cgagcagttc gatgactggc ttcgcattca
     1321 acgtgagcaa tttcgtttac aggtcattcg cgcgctggaa caactgggag cttttcatga
     1381 acacagcggc caactggctg aggcggaaca cgccatccgc caattgctcg aatatgaccc
     1441 tttgcgtgaa tcagcctatc gccaattaat gagtgtgctg gcgcgtgccg atcgacggag
     1501 cgctgcattg gatgtttacg aaacctgccg ccgggtgctg ggagcacaac ttggcgtagc
     1561 cctctctgtg gaaaccatca cactggctga acagatccgc gcgttggcgc cagtcaaatc
     1621 acatcgcgca caatcttttc tgccgcccgg cctgacgcgc ttctttggac gtcagccaga
     1681 atcggcacgc ctggtcgatt ttttatctcg ccgaacggtc aggctggtga ccctggcggg
     1741 cccaggcggt gtcggtaaga cccgtctcgc cctggaagtt gcccactgca tggcaggtgt
     1801 tttcgctcat gatatttgtt ttgttgaatt ggcaggtgta actgacgaaa aatctgtgga
     1861 tgacgcggtc gcagcggcgt tacatctgcc gacgagcacc ggccgctcct ccacggctgc
     1921 cattgtggat tacttgcacg ataagaccat gctcctggtg ctggataatt gtgaacatct
     1981 ggtgaaagcc tgcgcgcgcc tggtccaagc gctctgccag gctgccgagg gactgaccgt
     2041 ccttgcgacg agccgcatcc cgctgcacat tgacgaggag cacgtagtgc ggctggagcc
     2101 atttgccacg ccgaccatca atgaggcaaa acgactgacg gttgccgacg cgttgaactt
     2161 tgactctgtg caactgttca cagaccgcgc cgcgcaatcc ctgttgcaat ttcggctgac
     2221 cgacgcgaac gttgccgccg tcgtgcgcat ctgccagcat ctcgacggta tccctttggc
     2281 gatcgaaatc gccgccgcgc aaacccgggc cttgcccatc gaagcccttg ccgaacggtt
     2341 gagtcaacgc ttcgcctggc tgaatcggca ggtaagcgaa actctgcccc gccaacgcac
     2401 attacatgcc ttgatcgatt ggtcgtatga actcttaagc ccccaggcgc gttcagcctt
     2461 gcggtcattg tcggtctttg ccggcgggtg gacattggaa gccgctgagg cggtcactgc
     2521 ctcaggagca gcctgcgcag aaatccttac tgaactggtt gaccattcgt tggtgaagtt
     2581 tggcatggat gctggtcgtc aacgttacac catgcacgag acgatccgtc agtttgcgca
     2641 agcgcaattg cgaactgcga aacaaaaatc gaatgtattg gaacgtcacg ctcgttacta
     2701 tgcagggttt gtctcccacg cagcggagaa ccgaacgggg caaccattcc ctgagcgact
     2761 gcgaaccgtc gtgaacgatc acgataacct gcgccagggg ttcgaatggc tggctacgcg
     2821 tgatcgtgaa caagcgctgg cctttgttgc gcaactgggg acgaagttga atttctggga
     2881 gttgggcggc ttcttccagg agggacgtcg ttggttgcaa cgcgccttgg aaggcactga
     2941 ggattcggtt tcgatcgaac gtgcacgcgc cctgctggca gctgctgagc tttcgtctgc
     3001 gatcactgat ttcgaattgg ggttacattg cgttcagcaa gcccaacatt tgtttcagca
     3061 actcggcgat cagcagggga tgattgatgc caggttgacg tattgcaatc tagctgggct
     3121 tgccggcaaa gacacaaaga tacaggcaca ggctgaagaa gcgcttcgga tggccgagca
     3181 actctcctat acttttggga tagcgagggc caggaggata ctgggatcca ttgcttacga
     3241 tgccgacatg cctgaaacag cacttcaaca tcatctcccc agtgtcgcgc tatggcgcga
     3301 attggagaac ccgtttgaac ttgcaaatgc cattaataat cttggtgtag tcttgatgga
     3361 aatcggcgaa tacgtggaag cgagacaggc attcgaggaa actcgggaga tatttcactc
     3421 gctcggatat cagcgtggcg tcgccctcgc catgcacaac ctgggagaaa cggcttataa
     3481 gatgggggag tatgggaatg ccagggaaca cctctccgaa tcccttcgta ttcgccagca
     3541 tttgggcttg ccacgtggtt atccctactc gtttgaactt cttgctcagg tcaacgataa
     3601 ggaagggcgc taccaaaaag cagttcaact tctcgcggca tccgaagcct tgcgggttcg
     3661 cattggcgca ccgctcgaac aagtcgctca gaagcatgtc acagctgttt tagcaagcgc
     3721 acgccatcac cttggggatg tggcctatga actggcatgg tctaaaggag cggcgatgac
     3781 caccgaacaa gcgatcacgc tcgcgttgag ttgagagggt gctatttaag tgccaaaaac
     3841 aaactccccg gctttttagc tggggagttt gtttttctgt tcagtgattt ttaggcagct
     3901 actgcgggcc tgaatttata tccataaacg atcaagcccc gctcgtcgcc atcgttgcgc
     3961 attttgcgag ttaccatttc gacgggcatg ccgatctcga cggcgttatt gtccacgtcg
     4021 gtgagttggg cagtgatcat ggggccttca tccagtttaa tgagggcgac ggtgtaaggg
     4081 gcattggcat cgtaaccgct gggtgcttcg taaaccgtag tgtaagagta cacttcgcct
     4141 ttgccgctaa aggtataaag gtctttggct tcatcgccac agtttgggca tacatcgcgg
     4201 ggaggaaaaa ttttataatc gcaatggggg cagacttcgc cgaccaatcc atagcgctgc
     4261 tttttgagtc tccaatgtct tgggatttcc atgaaataag ttcctttctt cacatgtaat
     4321 tctacgcggg gagactctca aagactctca gggtcgtttc ctttctaaaa tgaaaattag
     4381 actttattaa gggttccaat cattttcaac aacctgtcga aaaataggga gaaagagcaa
     4441 ggaagtagag gcatccacga aggaatcccc ccaaatctaa ataaaacagc gtggacccaa
     4501 tcgggtccac gctgccggta caaagaaata cgctagtcca aaagttcctt tggaacattg
     4561 ccgccatttc gggcgacttc ctgcaggact ttcttgtgaa gccatgtgtt cattttcgcc
     4621 gaatcattca tcaattcagg cgggcagcca agttcgattg ccatttcctt gcggtcttcg
     4681 aaactgcttt ccaggtcaag gagtttcagc aggtcaacaa tcgatgtttt ccaattgagt
     4741 tttgccgggt tcctggcggc aagatcctca agtctgctga cgacatcgac ttctgaaatt
     4801 ggggttgcga ccggcgcttt tggcacagtc acaggaacgg gagcgggttt gggggtggat
     4861 gccggtctgg acgttgtcgt tggtgcaggt ttggcaggtg cggttgcttt cggtgccgga
     4921 gcggcggctg gcttctggga tacggtagcg gttgctccgc cctgagcatc cttatccttc
     4981 tttagcccaa gcttttcgag aatttttgag aataggccca tggtatttcc tttcgattca
     5041 attgcatgga agtggggtac ggagtcatga aaatctttcg ttcatgagat cctttacgat
     5101 tattataatt tacaatcggg taattttttt aagacaattt tagagttaag taagaattta
     5161 gagagaatgt caacgcgaat atattcaggg attaaggttt tttcaagcgc gagctggagg
     5221 tagtttgcta tcctctgtat catttataat gacctctcat gaaaatcttg ttgctgaatc
     5281 aagcatttgt ttctccagaa gaaccgggac atactcgcca ttttgaaatg gctcaatatt
     5341 tactcaaaca cgggcacgaa cttgtcatcg ttgccagtga tctgaattac cagaccggtc
     5401 aacgcattgt caaacaccgc ggcgtgttca ccgagcagtt taccgacggt gtgcgaatcc
     5461 ttcgttcgta tatctatccg gaattgcacc gcagttattt ttggcgaatc atttcctttt
     5521 ttagttttat gttcagctcc gtgtggacgg cgttatttgt aaaagacccg gacattatca
     5581 tgggcactac tccaccgatt tttcaagcgc tttctgcatg gtttgttgcc ttcttgcgac
     5641 gcaagccatt tcttctggaa gtgcgagacc tttggccaga attcggcatc agcatggggg
     5701 tgattacaaa tccccttgtc attgcgcctg cgcgctggct ggaaaatttc ctctaccgac
     5761 gcgccaccca tatcctggtc aattcacccg cctataaaga atatctgctc ggcaaagggg
     5821 tgcctgaaaa gaagatcacc ttcattccct acgggacgga tatcgaaatg ttcaatccga
     5881 gtatagatgg tgcacacatt cgaaagaaat atggactgga gagcaaattc acagtccttt
     5941 atgcaggggc attggggcag gcaaatgaca tcgacacaat cgtgcgtgcg gccacgcgct
     6001 tgcaggctga atcacacatt cgctttgtcc tctttggcga tggcaaggaa cggacgcggt
     6061 tggaggtaga agtccggaat cgagcgttgg ataacatcat ctttgcgggt gccgtcccaa
     6121 agaaagagat gccgtctgtg ttggcctcgt cagatgtgtg tctggcgatc ctgcaaaata
     6181 tacccatgtt tcgcacgacc tatccgaata aggttttcga ttacatggcg gccgggcggg
     6241 ctactgtact ggtcattgat ggggttattc gcgaagtgat cgagtcctct aacgggggaa
     6301 tctatgtcaa ccccggggat gaccaattgc ttgccaagac aattctcgaa ctttcaaaag
     6361 atcccgagcg cacgaatcaa ctgggagaaa acgcgcgcgc ctatttggtc aaacatctcg
     6421 accggcgaga caaattgaat gacactctgc atctgttaca aaatttagtg aacgtatgaa
     6481 agtctttgtc acgggtgcga cgggattcac cggctcacga gtcgtgcctc tgttgctgcg
     6541 acgtggatac caggtgcgat gtctcgcgcg cacgacgagt gatcgctccc ctctgccgca
     6601 gcccgagatt gagtgggtac agggagattt atcagacgcc gcagcgttga ccgcttcgat
     6661 gcaggggaca gatgccctcg tcaacgttgc ctcgctgggg ttcgggcatg ctgagtcaat
     6721 tgtcagcgcc gccaagaatg caggtatcca acgtgctgtc tttatcagta cgacagctat
     6781 tttcactcag ctaagcgcaa agagtaaaaa ggtccgcctt gctgcggagc atgccattga
     6841 aagcagcgga ctgaattaca ccatcctgcg cccgaccatg atctacggaa gcccacgaga
     6901 ccggaatatg tggcggctga ttcgattgat gcggtattcg ccgattatcc ctgtctttgg
     6961 agatggaaat tatctacaac agcctgtgta cgccggtgac gttgcccaag cgaccgtgaa
     7021 tgttatcggg aatgagcaaa cactttgcaa aagctacaac atcgccggga agcatccgct
     7081 tacttataat gaggtcattg aaacgatcgc gagacaaatg aagcgacgcg tgataaagct
     7141 ccatttgcct tctcaaccgg tcgtagcact attgagatta tttgagcgga ttcggattcc
     7201 actgcctctc aagtcggagc aggtgctgcg cctgaatgaa gataaggcat tttcctacac
     7261 agatgcggag aaagactttg actattcgcc tttggcattt gaagatggca taaaacttga
     7321 gttggaatat ttacgggggc attgaaatgc tgcgagtcgc tgtattttgc ctgttggttg
     7381 ctatctccta tgtcggcgtt ttctatatcc gccgcctggc attgcggcat aaaattatgg
     7441 accttcccaa tgaaggcagt tcacactcca agcccatgcc gctaggcggt gggttggtga
     7501 tcgccattct tgttcttgcg gtaggcgcat gggtggcaaa tgaaatcgat tggaagcgca
     7561 gcttgattta tattgcttcc ggttctgtcc tggcctggat gggctggcat gacgatgtgt
     7621 atcctctgcc cgctcgtttc cgcctgcttg ttcaaggaat ggtcgcggcg atttccatct
     7681 ggggcatggg gtatttcaaa gtgctcacca tcgcttcggc cggcgagagt caattgggtc
     7741 tatttgggat cctcatcacc ttcctttgga tcgtcggttt gatcaatgcc tttaacttta
     7801 tggacggcat cgacggcatg gcgggagggg tggcgttcag cgcggggcta ggctggatat
     7861 ggctttcact aaatgtaaat aacatgtttg cattttgggt ggctctcgca attgcagcca
     7921 gcagtttggg ctttctcgtg cataactggc cccccgccaa aattttcatg ggcgatgtcg
     7981 ccagcacatt tttaggctat accttcgctg ttttgtcgct catcgtctct gaccaacgcg
     8041 gagatgccct gctgatcggc acactcctga tgtggactgt cattatggat gcgggggtta
     8101 ccttcctggc gcgtttgttc aaacgcgaga atgtgctttc gcggcatcgt tcccacttgt
     8161 tccaacgtct ggtcatcagt ggctacaaac actcgacgat cagttcgttg tatattgttt
     8221 tgaccctcct tgccggctac ctttctcaca tctggcttct ggggagttat atcgctccgc
     8281 ccttgatatt tgccggtctc gttttgatct ggagcttgtt aacttttcat gctgcgcgct
     8341 tgcaaaatcc ttagtgagtg ttttagattt agtccttgtt gaatgataaa tagataggtc
     8401 tggtacagaa acttgagtat tatatgaatt cgggatcata aaatgaccaa gatgagacca
     8461 tttcttgtat tgttgtcctt atctgcatac tgtctttcat cttgcaatgc ttcaaaaaga
     8521 attcgaattg aatgcccaaa tatagaaact aatctttcca gtttaaagtc aagcggctcg
     8581 ctaatcctgg atggcgacgg aacttatctc aggaatatgg attcgctagc tacgacaaaa
     8641 ttaaacaaac cagatgaagg cttatattat tttgcttttt cacctaaacg ggattggatt
     8701 tcttatgttt caagtttaac ggatgatctt cttatcgcaa atgtagaaag tgaagttatt
     8761 aaggctatac tttgggaaga tgattgggct tatgcaaact ggcttaatga agagcaattg
     8821 ataatttctc tgatccaaaa agatcaaacg tcaaacagtt caaattttct tgtcttaaat
     8881 ccttttacaa atgaaagaaa tcttttgatt gcggagtttc ctcaaatcta ttacatgcaa
     8941 ccatggagaa tcattgaata tagcgctcaa attgaca
//
LOCUS       DASTJP010000127         3734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_452121, whole genome shotgun sequence.
ACCESSION   DASTJP010000127 DASTJP010000000
VERSION     DASTJP010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452121"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFQ23_03945"
     CDS             complement(<1..332)
                     /locus_tag="VFQ23_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905760.1"
                     /translation="MKKLTLFALVSVLISSCLPRDMQIPQSPLLSTLERKSGLIAYIG
                     ADGNLFVSDQAGGNLKQLTNDAKMPQSQSEPYRFYQYPTWSQDGNQLAFVSSSNENDV
                     ATSELLVA"
     gene            complement(329..1591)
                     /locus_tag="VFQ23_03950"
     CDS             complement(329..1591)
                     /locus_tag="VFQ23_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9905761.1"
                     /translation="MKRKIIFPSLVLIISVVLSACIGLIPLEDNTASGDFGPAYSSQE
                     HQTRVFDAIWSHLQNNYIYYESASVDWDALNKKYLERINSGLSNEEFTTLIQELEAEL
                     PEGSMTYQSRAERIEAETSDLSTYDGIGAFIGFREDPVPHIILLSVIEGSPAELAGLK
                     AHDSIFEIDGNPIQLEEGTNAVNRIRGPAGSSVTLNVQSPGESERSIELQRAKLTSTG
                     ALEAELIPGTNFGYLLLPPIGYENLVEDVLASLQSFSTNRTMEGLILDLRIAGSARGW
                     PLEELFTLFHNGDAGEFYNNQNQKQVVSITGQDQFNSQSVPLVILVGNNTTGSPEILA
                     ASLQAADRAEIVGTTTPGEIESTASFYLPNGSRVFIESASFRLSNGDELGTSGVKPDV
                     LVEADWDEILPDADPVLDAAVELLDTQQ"
     gene            complement(1940..2182)
                     /locus_tag="VFQ23_03955"
     CDS             complement(1940..2182)
                     /locus_tag="VFQ23_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905762.1"
                     /translation="MNESIQIGDRVKVFLDSKFWKSEGWFEGVVVRIDPYSEHRSFFW
                     VELDQDALALLGSGTKLISVLNPKNIQKIPAGSIPS"
     gene            complement(2191..2610)
                     /locus_tag="VFQ23_03960"
     CDS             complement(2191..2610)
                     /locus_tag="VFQ23_03960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET9905763.1"
                     /translation="MAEKKSKLETKAKKFIRQEYAIWLTTIGANLTPQPRPVWFVWDK
                     DSFLIFSQPQAHKLNHIRQHPNVSLNFNADKDIDQDVIVYLGTARIDPDAPPVHKMRA
                     YLKKYTAGIESMGATVEQFSQAYSVAIRVTPTSLRGW"
     gene            complement(2625..2807)
                     /locus_tag="VFQ23_03965"
     CDS             complement(2625..2807)
                     /locus_tag="VFQ23_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905764.1"
                     /translation="MKLAIQALCVMFFLAGLIFFLQGINVIPGSYMTGDPQWAINGSV
                     MMVVTVIIFFWINRRH"
     gene            2947..>3734
                     /locus_tag="VFQ23_03970"
     CDS             2947..>3734
                     /locus_tag="VFQ23_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET9905765.1"
                     /translation="MKFKLQAPFQPMGDQPEAIRGLVDGVNARMKHQVLLGATGTGKT
                     FTIASVIEQLQMPALIMAHNKTLAAQLYAEFKEFFPENAVSYFVSYYDYYQPEAYVPR
                     HDLYIEKETEINEEIERLRLAATTALVSRRDVIIVASVSCIYGLGSPEEFGKGTVRLK
                     VGELTRRNALLRQLIESQYQRNDMELRSGTFRVRGDTLEIIPAYEDKRGYRITFFGDE
                     VERITRFSPLTGEIFAEMEDVAIYPAKQYLTDSDRLKDAIHNIEA"
BASE COUNT          937 a          918 c          903 g          976 t
ORIGIN      
        1 tttgctacca ataattcaga tgtagcaacg tcgttctcat tgctggaact cacgaatgcc
       61 agttgattcc catcctgtga ccatgttgga tattgataaa aacgatacgg ctccgactgg
      121 ctttgtggca tcttagcgtc attggtcagt tgtttcagat ttccccccgc ttgatcagag
      181 acgaatagat taccgtccgc gccgatataa gcgattaagc ctgatttgcg ctcgagggta
      241 gaaagtaatg gactttgcgg aatctgcata tcgcgcggta agcaggatga aatcaagacc
      301 gagaccagag caaataaggt taactttttc attgttgtgt atccagcaat tcaactgcgg
      361 catccagaac cggatcagca tccggtaaga tttcatccca atccgcctcc accaggacat
      421 ctggcttgac tccgctggtt ccgagctcat cgccatttga caatcggaac gacgcgctct
      481 cgatgaagac gcgggagccg ttcggcaggt agaacgaagc cgttgactca atttcaccgg
      541 gggtggtggt accgacaatc tctgctcggt ctgcagcctg caggctcgcc gccaggattt
      601 ctggtgaacc cgtggtattg ttcccaacaa ggatcactaa cggaaccgat tgcgaattaa
      661 actgatcctg cccggtgatg ctaaccacct gtttctgatt ctgattgtta tagaactcac
      721 ccgcatcacc attatgaaat aatgtaaaca attcttcaag cggccagcct cttgctgaac
      781 cagcgatccg caggtccagg atcagccctt ccattgtgcg gtttgtactg aatgattgca
      841 ggcttgccaa cacatcttct accaaatttt catatccgat gggtgggagc aatagatacc
      901 caaaatttgt ccccgggatt agttctgcct caagcgcgcc tgtgctggtc aacttggcgc
      961 gctggagttc aatcgaacgt tcggactctc cggggctttg aacattaagc gtcaccgaac
     1021 ttccggcagg accgcgaatc cggttgactg catttgtccc ctcttcaagt tggataggat
     1081 tgccgtcaat ttcgaagatg ctatcgtgcg cttttaagcc agccagctct gcgggcgagc
     1141 cttcgataac agatagcaaa ataatgtgcg gaaccggatc ttcacggaag ccaatgaatg
     1201 ccccaatacc atcatacgta gagaggtcgg aagtctcggc ttcaattcgc tctgctcgcg
     1261 attggtaggt catacttcct tcgggaagct cagcttcgag ctcctgaatt agcgtagtga
     1321 attcctcatt ggataacccg gaattgattc gctctagata cttcttgttc aacgcatccc
     1381 aatctacaga cgcactctca taataaatat agttattctg cagatgcgac cagatcgcat
     1441 caaagactct tgtttgatgt tcctgagatg aatatgctgg tccgaaatct cctgatgcag
     1501 tgttatcctc cagggggatt aacccaatac aagccgataa gacgacactg attatcagaa
     1561 cgagggatgg gaaaatgatt tttcttttca taacaaatcc tctacatcta aagcaaaaaa
     1621 gacttctaag aatagcatac aaaaattaaa tccccatgat taaatacgta atatttcgtg
     1681 gtttgtattc ctgtcgagac tttccaaatt ttactcctgt ttcaggtgaa ccaagtggtc
     1741 tgtttcaagg gaatgtcgaa tttcgggtgg aagccacaca actgcctgat gtgctcgagt
     1801 aaatcacagg cggcgctagg gagcccaccg agtttttgag tttttttcca cctgccccga
     1861 acgcaggtac ggggtcaaag ggcccagtgt tctctgtggc gaagtcgatt ttcactcact
     1921 ctcttggcta ctccctgtgt caagaaggaa tgcttccggc tgggattttt tgtatatttt
     1981 ttggattcaa tacggaaatg agtttggtgc cggatccaag cagcgccagc gcatcctggt
     2041 ccaattccac ccagaaaaag ctgcggtgtt cagagtatgg atcgatgcgg accaccacgc
     2101 cctcgaacca gccttcgctc ttccaaaact tggaatcgag gaagactttt actcggtcac
     2161 cgatctgtat ggattcgttc attcacgctt ttaccagccg cgcagtgagg tgggagtcac
     2221 ccggatcgcc acggaatagg cctggctgaa ctgctcaacc gtcgcgccca tcgactcgat
     2281 gcccgccgtg tactttttga gataggcacg catcttgtgc accggcgggg catcgggatc
     2341 gatccgagct gtgcccagat aaacgatcac atcctggtca atgtctttat ctgcgttgaa
     2401 attcagcgag acattggggt gttgtctaat gtggtttaat ttgtgcgcct gtggctgact
     2461 aaagatcaga aatgaatcct tgtcccagac aaaccagacg ggtcggggtt gaggcgtaag
     2521 gtttgcgcca atggttgtca gccaaatggc atattcctgc ctgataaatt tcttcgcctt
     2581 cgtttccagt ttagattttt tctctgccat gtgtactcca tccattaatg acggcggttt
     2641 atccaaaaga atatgattac agtaactacc atcatgaccg agccgttgat cgcccattgc
     2701 gggtcacccg tcatatagct gcccggaatg acgtttattc cctgtaggaa aaagatcagc
     2761 ccggcaagaa aaaacatgac gcaaagggcc tgaattgcta gtttcatatt gaatggtttc
     2821 cttggatttt gattgcgaga ttatacacgt ttcattttcc tgaacggttc gttcttatcc
     2881 ttgagagcaa aacgaggtgt atgatataat agaacacccg ttcagaacac tcaactaaaa
     2941 aatcctatga aatttaaact ccaagcaccc tttcagccta tgggcgatca gcccgaagcc
     3001 atccgcggac ttgtggatgg cgtcaatgcg cggatgaaac accaggtttt attaggcgcg
     3061 accggtactg gtaagacgtt tacgattgcg tctgtgatcg agcagttaca aatgcctgcg
     3121 ctcattatgg cacataacaa gacccttgcc gcgcagttat atgccgagtt caaggagttc
     3181 tttcctgaga acgcggtttc gtattttgtt tcttactacg attactatca gccggaagcc
     3241 tacgtcccac gacatgacct gtacattgag aaagaaacag agatcaatga agagatcgaa
     3301 cgcttgcgcc tggctgcaac gacggcgctg gtttcccggc gcgatgtcat tattgtggca
     3361 tcggtatcct gcatctacgg tttgggaagc ccggaggagt tcggcaaagg gacagtcaga
     3421 ctcaaagtgg gggaactgac gcgcaggaac gcgttgctgc gacaattgat cgagtcacaa
     3481 taccagcgca acgatatgga actgcgctcc ggtacgtttc gggtgcgcgg cgacacgctg
     3541 gagatcatcc ctgcgtatga ggataagcgc ggctatcgca ttacgttctt tggcgacgaa
     3601 gtggagcgca tcacgcgctt tagtccgtta actggtgaaa tatttgcaga gatggaagac
     3661 gtcgcgatct accccgccaa gcaatattta accgatagtg atcggttaaa agatgctatc
     3721 cataatatcg aagc
//
LOCUS       DASTJP010000128         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_29475, whole genome shotgun sequence.
ACCESSION   DASTJP010000128 DASTJP010000000
VERSION     DASTJP010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29475"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VFQ23_03975"
     CDS             complement(<1..1246)
                     /locus_tag="VFQ23_03975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9905766.1"
                     /translation="MNFLKLFIEAFIPISSIILAVTGIIQLRSGDVTTGVVCIFLAIA
                     TFVFSMRRLEKNTVEQEKRVSLKPASVPSVSWVIVVGLVVISVIYVADNVKSAVTDRI
                     AVIAWVLAMLLGLFLTWWQTLGLFLTNKANLLKEKIRTNRKEIAILFLILFIAFLLRT
                     VSLTSHPYPWSGDEASIGMEARRIIHGEITNYFDTGWSSQSNWSFFPTAVSEIVFGQN
                     ILAVRLTSVLAGILAVLFTYLTARVLFNPTIGLIAAAFLATLPYHVHFSRIGVHNIVD
                     SLMSSLLFWLIAKGLKEDDPRFYYTAGVVGGLSVYTYAGTRLVLILSAITFLFVILRQ
                     RGYLAVHWRHLTAFASGVILSMAPQAAYFARHPDIFFGRLGQEGILFNGWLARRALET
                     GQGQWDILLDQFKSTILVFVASP"
     gene            complement(1296..>3273)
                     /locus_tag="VFQ23_03980"
     CDS             complement(1296..>3273)
                     /locus_tag="VFQ23_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905767.1"
                     /translation="TRAMSALVTLIAAIAVGLVLRDIFKLKYWWTGTLFLSITPAWFL
                     HSRTAFETAEFVAFYAGTLCAYLYDRYKSPGYLNLTVFMGALAFYTYSPGQVIVPLTA
                     LGLLISDWRYHWENRRAVLIGLAFAVILAVPYIRYIINNPSTPFAHLHTLWSYWYENI
                     PFSEKLIRYLSEFGVGLSPWYWYVPNNRDLARHLMKEYGHILIVTLPFALWGLIQTLR
                     NLHNSAHRAILLALLISPAAAALVQISITRTLVFVVPAALLTAIGLEQVLGWLEDPKK
                     HMAELSEGAGTSKRRLIAALIVVLVGIPVALVSTENTNRFILLSLTFILGIQVSGVLE
                     RWARSITGAEAAPPTLWKLSPAFIAIATFALLAGANIRMLNDAIRNGPLWYRDYGLGG
                     MQYGGFQIFNIIDEYLNDRPDSKIIFSPDWANGADVLARFFLDNPSSIRIGSVQGHIT
                     QLSPLDDNTLFIMTPQEYNAIKENPKFAEVDVETIIPYPDDSPGFYFVRLRYVDNIEE
                     IFAAEQSVRQALRESILKIEGQDVKLRHSYLDSEDQAKWIALVFDNDPFTVTKTLETN
                     PFVLEMIFPETRTINGFSIIIGSANVQITLNAYPTADAEPLVYTFEGQGAQNQPELSF
                     NLPEAVQVQVLRFEVLDLRSSDRAKVHIWELKLR"
BASE COUNT          946 a          780 c          772 g          775 t
ORIGIN      
        1 cgggggatgc aacaaaaacc aatattgtgc tcttaaattg atcaaggaga atatcccatt
       61 gaccttgacc cgtctctagt gcccttcgcg ccagccatcc attgaatagg atgccttcct
      121 gccctagacg cccaaaaaaa atatccgggt gtcgtgcaaa gtatgcagct tgaggtgcca
      181 tgctcaggat gactccggag gcgaacgcgg tgagatgtct ccaatgaaca gccaggtaac
      241 ctcgttgcct gaggataacg aataagaaag taattgcact gagaatcaga actagtcttg
      301 tgccggcata ggtatagacg ctgagcccac caacgacgcc cgccgtgtaa taaaaacgcg
      361 gatcgtcctc tttgagtcct ttggcgatca gccaaaaaag cagcgatgac attaagctat
      421 ccacgatatt gtggaccccg attcgactga aatgcacatg ataggggagc gttgccagga
      481 aggccgccgc gatcagtcca attgtcgggt tgaacaaaac gcgagccgta agatatgtga
      541 acaaaacggc caatatacct gccagcacac tcgtcaggcg aacagccaga atgttttgac
      601 caaagacaat ttctgaaacc gctgtcggga agaaagacca gttcgattga ctggaccagc
      661 ctgtgtcgaa gtaatttgtg atttccccat gtatgatgcg ccgagcctcc atgccgatcg
      721 acgcttcatc gccggaccac ggatatggat gggatgtcaa actgaccgtc cgcaaaagga
      781 aagctatgaa cagaataaga aatagaatag ctatttcttt acggttcgtt cgaatttttt
      841 cttttagtaa atttgcttta ttcgtaagga acaatccgag agtttgccac cacgtaagaa
      901 agagtccaag caacatggca agcacccagg ctatgactgc aatgcgatcg gttacggcac
      961 ttttgacatt gtctgccaca taaataacag agattactac cagtcctaca acaatcaccc
     1021 aacttacgct cggcaccgat gcgggcttta gagagacacg tttctcttgc tccactgtgt
     1081 ttttctccag ccgtcgcatg ctgaaaacga aagttgcaat cgccaggaaa atacaaacta
     1141 cacctgtggt gacatctccg ctgcgcagtt gaattatccc ggttacggcg aggatgatgg
     1201 aactgatcgg tataaatgcc tcaataaata attttagaaa attcataagc taattgtggg
     1261 ctagaaggaa tccaagatga atacttaaaa ctgcatcagc gcagttttag ctcccaaata
     1321 tgaacctttg cccggtcaga agagcgaaga tcgagtactt cgaagcgcaa tacttgaacc
     1381 tgtaccgcct caggcaggtt aaatgaaagt tccggttggt tctgcgctcc ttgtccctca
     1441 aaagtataaa ccagaggttc agcgtcggcc gtgggatagg cgttcaaggt gatctggaca
     1501 tttgccgaac caataatgat cgaaaatccg ttgatcgtgc gggtttcggg aaagatcatt
     1561 tccaggacaa atggattcgt ttctaatgtt tttgtcaccg tgaagggatc attatcaaat
     1621 actaaagcaa tccactttgc ttgatcttca ctgtccagat aagagtggcg tagtttaacg
     1681 tcttgtccct caattttcag gatagattcc cgaagcgcct gacggacaga ctgctctgcc
     1741 gcgaagattt cttcgatatt gtctacgtaa cgcaggcgga caaaataaaa gccagggctg
     1801 tcatcggggt agggaataat cgtttcaaca tcaacctcgg caaattttgg gttctctttg
     1861 atggcgttat attcctgcgg tgtcatgatg aagagagtgt tatcgtccag aggcgataat
     1921 tgagtaatat gcccctggac gctaccgatt cggatagagg acggattgtc aagaaaaaaa
     1981 cgtgctaata catctgcacc gtttgcccaa tctggtgaaa agataatctt tgagtctggg
     2041 cgatcgttca gatattcatc gatgatattg aagatttgaa agccaccata ttgcattcct
     2101 cccagtccat aatctctata ccagagcggg ccattccgga tggcatcatt taacattcgt
     2161 atgttcgcgc ctgccaacag ggcaaaagtg gctatcgcga tgaatgcagg tgaaagcttc
     2221 catagtgtag gtggagcagc ctctgcgccg gtgattgatc gcgcccacct ctccagcaca
     2281 cctgagactt gaatgcccaa aatgaatgtc aagctcaaaa ggataaagcg gttagtattt
     2341 tctgttgaaa ccaatgcaac aggaattccc accaggacga ctatgagagc tgcaatcaga
     2401 cgccttttgg aagttccggc accctcacta agctcagcca tatgtttttt cggatcctcc
     2461 aaccagccta gaacctgttc cagccctatc gcggttagta aagccgcggg aaccacaaaa
     2521 acgagtgtac gtgtaatact gatctggaca agcgccgctg ctgccggaga gatcaaaagc
     2581 gcaagcagga tcgcacgatg agccgaattg tggagattac gcagtgtctg aatcaagccc
     2641 catagcgcaa atggtaatgt aacaataagg atatgtccgt actctttcat caggtgacgt
     2701 gcaagatcac ggttgttggg gacataccaa taccacgggc tcaggccaac cccaaattct
     2761 gataagtacc gaattaactt ttctgaaaaa ggaatatttt cataccaata cgaccatagt
     2821 gtatgcaaat gcgcgaatgg cgtgctcggg ttgttaatta tgtaccggat ataaggcaca
     2881 gcgaggatca cggcgaatgc cagcccgatc agaaccgcgc ggcgattctc ccagtggtat
     2941 cgccagtctg agatgagcaa tccaagtgca gttaatggaa cgataacctg tccagggctg
     3001 tacgtataga aagcaagcgc tcccatgaaa accgtaagat tcaaataccc cggcgattta
     3061 tatcgatcat aaagataggc gcataacgtc cctgcataaa atgccacaaa ttcagcggtt
     3121 tcaaacgccg ttcgcgaatg taaaaaccag gcaggcgtaa tcgacaaaaa taatgtccct
     3181 gtccaccaat acttcagttt gaaaatatct cgcagaacca gcccgacggc gattgcagca
     3241 atcaaggtca ccagggcaga catggcccgc gtt
//
LOCUS       DASTJP010000129         3903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_874454, whole genome shotgun sequence.
ACCESSION   DASTJP010000129 DASTJP010000000
VERSION     DASTJP010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874454"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFQ23_03985"
     CDS             <1..395
                     /locus_tag="VFQ23_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905768.1"
                     /translation="KVGLFDEDPVTATAEDAEWAYRALRKGICIRYEPDVVLAHFGWR
                     DNTKREQQYRNYALSHGGFYGKYVRRGDLFMLARAAGHFARALRRWIRGTLAGDKELA
                     TLGKAYCLNMLPGILAGLRSKMRPEVLS"
     gene            395..1873
                     /locus_tag="VFQ23_03990"
     CDS             395..1873
                     /locus_tag="VFQ23_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HET9905769.1"
                     /translation="MEKHHVHAAGNAMAWKLAQMGGVKIIYLIRLLVLAVLLTPADFG
                     LVAIATAATGFLMSLTNFGLIPAVVQAANMDEVKYDSAWTFDVTRSLIVAGLTITFAS
                     MIANIFAEPRAIPIIQVLALRPLLESMASIKVAALNRDLSFRPLAYLRIVEAIFNTII
                     SIVLAKFIGVWALVLGSTGGAISMVITSYIVAPHRPRVSFNFREIRPLINFGGWVFLT
                     SLIAMAGNYGLRIAISRQLGAEGLGLYFIAMQLAYLPSEVASEAVGAVAFPLFARLQG
                     NISQATRAFRALFSGLAAVIFPVCALLIVLAPKLTHDVLGPSWAGTEEVIRVLSLVVM
                     MGIFGEVAVSVFKGFGQPYRIALLEAFQSSITISFVWVLTRRFGLVGSAMAWLPAILV
                     SQLLSIRFLHNILDHPLRGLQNSLVAVLAATGLCFIVAMTANFAIPGVVGLFTAAALG
                     GASTILLLWAADRRYKLGFADNFAMAFPQFASFLRLAPPKPD"
     gene            2159..2265
                     /locus_tag="VFQ23_03995"
     tRNA            2159..2265
                     /locus_tag="VFQ23_03995"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            2998..>3903
                     /locus_tag="VFQ23_04000"
     CDS             2998..>3903
                     /locus_tag="VFQ23_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9905770.1"
                     /translation="MKPIMVTGSPRSGTSWTGRIVSQSPFIRYVHEPFNIGGRPCHCG
                     IKFDSWFYYLSRKNRRLFDQHLNHTIFPAFSRIALVNLISEISQTRRIRPLFKYLQSY
                     LPHRVLLKAPFALFSTEALADNFRMDVIVLIRHPAAVVNSYKALNWSHPFSHFLSQAE
                     LMDDHLFPFRMEIEDFSKNEYDIVDQISLLWKLIHFMIHKFQKTHSDWVFVRHEDLAL
                     EPIEGFRTIFRRINLPFTRYTEAVIRAHNIGNPQSNTTHPYAIKQNGNQVVSKWKKAL
                     TSDEIYRIQRMVEPVSSEFYSEQDWN"
BASE COUNT          948 a          873 c          959 g         1123 t
ORIGIN      
        1 ggaaggtggg tcttttcgat gaggatccag tgacggcgac cgcggaagac gccgagtggg
       61 cgtaccgtgc cttgcgtaag ggcatttgca ttcgttacga gccggacgtg gttctcgcac
      121 attttggctg gcgggataac actaagcgcg aacaacaata ccgtaattat gctctgagtc
      181 atggcggctt ttacgggaag tacgtgcgcc gcggcgatct gttcatgctg gcgcgtgcag
      241 ccggtcattt tgcgcgagca ctgcggaggt ggatccgggg aactctggcg ggagataaag
      301 aactcgcaac actcggcaaa gcatactgtc ttaatatgct gccaggtatc cttgctgggc
      361 tacggtcgaa gatgcgaccg gaggtactgt cctgatggaa aaacatcatg tgcacgccgc
      421 agggaatgca atggcttgga agcttgccca aatgggtgga gtcaagatta tctacctgat
      481 ccgattgctg gtgttggcag ttctacttac gccggccgac tttggtctgg tcgctattgc
      541 gaccgcggca acgggctttc tgatgagcct gacgaacttc ggtttgattc cggctgtcgt
      601 ccaagcagca aatatggatg aagtgaaata tgactcggct tggactttcg atgtgacacg
      661 gtctctcatc gttgcaggac taaccatcac gtttgcttca atgatagcga acatttttgc
      721 agagccccgc gcgataccaa ttattcaggt gcttgcattg cgacctctgc tggaatcaat
      781 ggctagtatc aaggtagcag cgctgaatcg tgacttatcc tttagaccct tagcgtactt
      841 gaggattgtg gaagcgatct ttaacaccat tatttcaatc gtacttgcaa aattcatagg
      901 ggtttgggct ttggtgctgg gttcaaccgg cggtgcgatt tccatggtga ttacatctta
      961 catagtcgct ccccaccggc ctcgtgtatc ctttaatttt cgagagattc gaccgctgat
     1021 aaatttcgga ggttgggtgt ttctcacaag tctaatcgca atggccggaa actatggact
     1081 tagaatcgca atatctcgac aacttggtgc cgagggccta ggtctttatt ttattgccat
     1141 gcaacttgca tacctaccca gtgaggttgc cagtgaagca gttggtgctg tagcttttcc
     1201 actcttcgca cggttacagg ggaatatatc tcaggcaacc agagcatttc gcgccctgtt
     1261 cagcgggctg gcagctgtca tttttcctgt ttgtgcgctt ctcattgtac ttgcacccaa
     1321 acttacgcat gatgtattgg gtcccagctg ggcaggaaca gaagaagtta tccgggttct
     1381 ttcactggta gttatgatgg gtatttttgg agaggtggcg gtttcggttt tcaaaggttt
     1441 tggtcaacca tatcgaatcg ctcttttaga agcatttcaa tcatccatca ccatttcgtt
     1501 cgtgtgggta ttgacgagac gcttcgggct cgtaggctca gcgatggcat ggttacctgc
     1561 aattctggtt tctcagttgc taagtattcg atttttgcac aacatcctag atcatcccct
     1621 acgcggcttg caaaattctc ttgtggcggt tctggcagca acaggcttgt gcttcatcgt
     1681 agcgatgaca gcaaactttg cgataccagg cgttgttgga ctgtttaccg ctgccgcgct
     1741 tggaggtgcg tccacgatct tgctgttgtg ggcggctgat cgacgctata agctcggatt
     1801 tgctgataat tttgctatgg cctttccaca atttgcatcc tttcttcgtc ttgctccacc
     1861 aaaacccgat tagagtcact gaattgaacc cggttaccct ttagtgcttc gaatatgtca
     1921 caaaaaaccc ttgtttctgt gatcattatt tttttcaatg catctgactt tcttccgacc
     1981 gcgatagaaa gtgtgttagc acagacctac gatcattggg aactattact ggtggatgat
     2041 ggatccaacg atgggagcac tgagatcgcc aagagatttg tatccgcgca cccagcgcac
     2101 atgaaatact tggagcatga tggtcatgaa aatttaggta tgagcgcggc ccgcaatctg
     2161 ggcattcgtc aaacgaatgg tgactatatt gcattccttg atgccgatga ctactgggta
     2221 ccaaacaaac tagaagttca ggttcgaatc ctggatgctc agccagaagc tggaatgttg
     2281 tttggaagta caaagtattg gtttagctgg agtggccgtc cagaagatag ccagcgagac
     2341 tatgttccat cacttagggt aaagacacat actttattcg agcctccgtt attgctggtc
     2401 ctgttcttac agggaaaggc cgaagtaccc tgtacttgca gcatcctggt acgtcggtct
     2461 attgtagcgg agacgggtgg ctttgaagaa tcttttcgag gaatgtatga agatcaggca
     2521 ttttacgcaa aaatttgctt aagcacgccg gtcctggcca cagatgattg cctggcttgg
     2581 tatagacagc atacaaaatc aagcaactcg actgcaattc gaattggact aacacaagtt
     2641 ctacgtcaca gctttctcaa atggttagaa aaatattgtc aggaaaaaca agtgaaggat
     2701 acaatggtat ggtcggtcgt tcggcgccaa ttgtggctct acagtagtat ctctcagcag
     2761 cttccagggt ttgctcatta tattatccgt tggataagaa aatggatatt aaggttggaa
     2821 gaaagtcttc taccttcaag cttgatcgac cggctgtgga tacgcaaata attgggaatt
     2881 gagcgaggca aatgctatct tccagaatgc tctgttagcg tatgtgagta gattaatgat
     2941 aaggtttgct gtgttcattc tcgaataggt actctccgga ggtggttggt gttttcaatg
     3001 aagcctatta tggtaaccgg ctcacctcgc tcggggacat cttggaccgg gcgaatagtc
     3061 agtcagtcac cttttattcg atatgttcat gaacccttta atattggagg gcgaccttgt
     3121 cattgtggta tcaaattcga ttcttggttt tactatttgt ctcgaaaaaa taggcggctt
     3181 tttgatcagc atctaaatca tacaatcttt cctgccttca gtcgaattgc gctggttaat
     3241 ttgattagtg aaataagtca aacaaggcgg atccggccac tatttaaata tcttcaatcg
     3301 tatctccccc accgggtttt actaaaagct ccctttgccc tattttctac agaagcgtta
     3361 gctgataact tccgcatgga tgtaatcgtt ctcattcgcc atcccgctgc cgtggtgaac
     3421 agctacaaag cattgaactg gtcacatccg ttctcacatt ttctaagtca agctgagtta
     3481 atggacgatc acttgttccc atttcgcatg gaaatcgagg atttttctaa gaacgaatac
     3541 gatattgtgg accaaatctc gttgctgtgg aaattaattc atttcatgat ccataagttt
     3601 cagaaaacgc attccgattg ggtctttgtt cgacatgaag atctggcttt agaaccgatc
     3661 gaaggtttcc gaactatttt ccgacgcata aacttgccct tcacaagata taccgaagcc
     3721 gtcatccggg ctcacaatat cggaaatccc caatccaata ccactcaccc ctatgccatt
     3781 aagcaaaacg gaaatcaggt cgtgtcaaaa tggaagaagg ctttgacctc ggatgaaatt
     3841 tatcggattc aaaggatggt cgaacccgtt tcttcagagt tctattctga gcaggactgg
     3901 aat
//
LOCUS       DASTJP010000130         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1296972, whole genome shotgun sequence.
ACCESSION   DASTJP010000130 DASTJP010000000
VERSION     DASTJP010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296972"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1690
                     /gene="lysS"
                     /locus_tag="VFQ23_04005"
     CDS             155..1690
                     /gene="lysS"
                     /locus_tag="VFQ23_04005"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET9905771.1"
                     /translation="MSDYTSLEKIRLQKLEELRAEGIETYPTRAKRTHTSVEAIAAFE
                     AAELESANSETSPQVRVILAGRIRATRVMGKISFAHIEDGDGKIQLFFRANEIGKERL
                     ELFNKMFDLGDFIQAEGEMFRTKSGEATLHVHDFHLLAKAISPLPAAKDEKLEDGTVI
                     RHAALEDPELRARQRYADLAVNPDVRETFRKRAAIIKALRTFLDNKGFLEVETPILQP
                     LYGGAAARPFTTHHNELDQDMYLRISFELYLKRLLVGNLEKVYEIGRDFRNEGVSFKH
                     NPEFTQLEFYWAYADYEQVMALTEQMVSFTAQEVLGTQRITYKGQEIDLTPPWRRLKM
                     RDGILETSNIDIDEHKTAESLFSAIRTKHPGHTPDPKATRGKLIDFLVSEFLEPTLIQ
                     PTFLYDYPRDISPLAKSIPGNPLTVERFEAYMAGFELCNAFTELNDPLDQEQRFIEMG
                     RDYESGDEEQHPLDEDYLRAMRYGMPPNGGFGMGVDRLAMLLLDKHSIREVLLFPALR
                     SEE"
     gene            1705..2247
                     /locus_tag="VFQ23_04010"
     CDS             1705..2247
                     /locus_tag="VFQ23_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905772.1"
                     /translation="MDELLLIPTGLQKEFTVADLTPDQVQKIHQLLHDKQVIHAIKLY
                     REATGASLAEAKRAVEEMAFVEVTKPPSGVRSFDNPVLDSKIRSMLAKGKKIDAIKIY
                     REEYGIGLNEAKIAVERIEATMPRDSATGTPYESAIGKDPFADNDSAGRVRLIILSVA
                     LLVVLCSAGAFVLVLVSSSP"
     gene            2244..2630
                     /locus_tag="VFQ23_04015"
     CDS             2244..2630
                     /locus_tag="VFQ23_04015"
                     /inference="COORDINATES: protein motif:HMM:NF022700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HET9905773.1"
                     /translation="MTKGKWILYIGDILALAALTFLGFATHGETNLAFLPRMGAIFFP
                     LILAWFLIAPWLGLFQPEITSRPKQLWRPAIAMLVAAPLAGVLRSLILNTPVIPIFIL
                     VLTVTFALGMVIWRGLYYLFNHKMAI"
     gene            2707..3315
                     /locus_tag="VFQ23_04020"
     CDS             2707..3315
                     /locus_tag="VFQ23_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9905774.1"
                     /translation="MNEQALIQDAQKGDLDAFNTLILHYQDSVYNTALRILGDEDQAS
                     DAAQEAFISAFKSIGSFRGGSFKAWLMRTVTNACYDELRRQKRRPTTPLEPDTDDGEE
                     MDSPRWLADPEMTPDQKAEADELEHAIQHCLNDLPIEFKTVVVMADIQGMDYTEVATS
                     VRVPLGTIKSRLARARLRLRECLQGFSELLPASFRLEEESNA"
     gene            3315..4013
                     /locus_tag="VFQ23_04025"
     CDS             3315..4013
                     /locus_tag="VFQ23_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905775.1"
                     /translation="MNRRDIELLSAYLDGQLKPSDTTRLESRLKSDPELVAVMDDLRA
                     ARNLLRKLPQRRAPRNFTLTRKMVGQNPPMPRAYPFFRFATTIATLLLFFSFGLNFLA
                     PQMAAAPSFGMGGGGPGQDLFSSEQAQEPSIAAAPVEAPAATEAPLPSAEMLPMPTQI
                     STLESERSAVPPTEKIDATGNAVASDQSQVENPVPVPVSTGWQIALAGIAVVGALLML
                     LLRQSAFQRWRQKE"
BASE COUNT         1016 a         1081 c         1068 g         1018 t
ORIGIN      
        1 ttaaaatcct gaaagtcggt taaacacagg agcgtaaacg tatgccccgc gcacctgtgc
       61 gcggggtttt cttttgcccc ctcccgcctt cggctaggac gggggcgaac tttccctcat
      121 cctagccctt ctccaagaga agggggagca tcgcatgtca gattacactt cacttgaaaa
      181 aatacggctg caaaaactgg aagaactccg cgcggaaggg atagaaacgt atccaacgcg
      241 cgcaaagcgt acgcacacca gcgtggaagc gattgccgcg ttcgaggctg ctgaacttga
      301 aagcgcgaac tcggaaacct caccccaggt acgggtcatt ttggccggac gcatccgtgc
      361 cacgcgcgtg atggggaaga tctcctttgc tcatattgaa gacggcgatg gaaaaattca
      421 gttgtttttt cgggccaatg aaattggaaa agagcggctc gagttattca ataagatgtt
      481 cgacctgggt gactttattc aggctgaggg tgagatgttc cgcacgaagt ccggtgaagc
      541 tacccttcac gtccatgact ttcatctgct tgctaaagcg atcagcccac tcccggctgc
      601 aaaagatgaa aaattggaag atggcacggt aatacgtcac gcggccctcg aagacccgga
      661 actccgcgcg agacagcgtt atgccgattt ggcggtgaac cctgatgtgc gcgagacgtt
      721 tcgaaagcgc gcggcgatca tcaaagcctt gcggactttt ctggataata agggttttct
      781 cgaagttgaa actcccatcc tccagccctt atatggaggc gccgccgctc gtccgtttac
      841 cacccatcac aatgaacttg atcaggacat gtatttgcgt atttcgtttg aactgtatct
      901 caaacggttg ctggtaggga atcttgaaaa agtctatgag atcgggcggg atttccgcaa
      961 tgaaggtgtt tcattcaaac acaaccccga gttcacgcag cttgaattct actgggcgta
     1021 tgccgattac gagcaggtga tggcattgac cgagcaaatg gtctcgttca ccgcacagga
     1081 agttctggga actcaaagaa tcacgtacaa aggtcaggag attgacctta cacccccgtg
     1141 gcggcggctg aaaatgcgcg acggcattct cgagacctcg aatattgata ttgatgagca
     1201 taaaacggcg gaatctttgt ttagcgcgat cagaacgaaa cacccgggcc ataccccgga
     1261 ccccaaagcg acgcgcggga agttgattga cttcctggtt tctgagtttc tcgaacccac
     1321 tttgattcag ccgacttttc tatacgacta tccacgtgat atttcacccc tggcaaagtc
     1381 cattcccggg aacccattga ccgtggagcg tttcgaagcg tacatggcgg gcttcgagtt
     1441 atgcaatgcg ttcaccgagt tgaacgatcc cttggatcag gaacagcgct tcattgaaat
     1501 gggccgtgac tatgaatccg gtgacgaaga gcagcatccg ctggatgaag attatttgcg
     1561 tgccatgcgt tatggcatgc cgcccaacgg tgggttcggc atgggtgttg accgtcttgc
     1621 gatgctgctg ctggataaac actctatacg tgaagtattg ctgttccccg ccttgaggag
     1681 tgaagaataa atctatgatt tcgcatggac gagctgctgc ttatccctac gggtttacaa
     1741 aaggagttta ctgtggctga cctgaccccc gatcaagtac aaaagatcca tcaattgctc
     1801 catgataaac aggtgatcca tgccattaag ctctatcgtg aggcaaccgg agccagtctc
     1861 gcagaagcca agcgggctgt cgaagagatg gcattcgttg aggtgacaaa accgccgtca
     1921 ggggtacgga gctttgacaa tcccgttctt gatagcaaga tcagatccat gcttgcgaaa
     1981 ggcaaaaaaa tcgatgcgat aaagatctat cgtgaggaat acggaattgg gttgaacgaa
     2041 gccaaaattg ccgtggaacg gatcgaagcc accatgccac gcgactctgc taccggtaca
     2101 ccttacgaat cagcgattgg caaagaccca tttgccgata atgacagtgc aggcagagtc
     2161 cgattaatta ttcttagcgt cgcgctactt gttgttttgt gcagcgccgg agcattcgtt
     2221 ctagttttgg tgtcatctag cccatgacga agggcaaatg gattctgtat ataggagata
     2281 ttctcgccct cgccgcctta acttttcttg gtttcgccac gcatggggag acgaacctgg
     2341 cttttttgcc ccgtatgggt gcaatcttct ttccactaat acttgcgtgg tttctcattg
     2401 ctccttggct tgggcttttt cagcctgaga ttacatccag gcccaaacaa ctttggagac
     2461 cagccattgc catgctggtt gcggctccgt tggctggtgt cttgcgcagt cttattctca
     2521 acacgcccgt gatcccgatc tttattcttg tcttgaccgt aactttcgca ctgggaatgg
     2581 tcatctggcg aggcttatat tatctcttca atcacaaaat ggcaatttag cgaagccttc
     2641 acaggttttt tagacctttg tgagcgttgt gcgtaaaaaa attctcccag tgataaaata
     2701 ctcttgatga atgaacaagc tctgattcaa gatgcccaaa aaggggatct tgatgccttc
     2761 aatacgctga tcctgcacta tcaggatagt gtttataaca ccgcgctgcg cattctgggg
     2821 gatgaagatc aggcatcgga cgcggcccag gaagctttca tttcggcgtt caagagcatt
     2881 ggctcctttc gtgggggatc gttcaaagcc tggctgatgc gtacggtgac aaatgcctgc
     2941 tatgatgagc ttcgccgtca aaagcgccgt ccgacaacgc ctctcgagcc tgacactgac
     3001 gatggcgaag agatggactc cccgcgctgg ctggcagacc ctgagatgac cccggaccaa
     3061 aaagcagaag ccgacgaact tgaacatgcc attcaacact gcctcaatga cctgcccatt
     3121 gaattcaaaa ctgtcgttgt gatggcagat attcaaggca tggactacac ggaagttgcc
     3181 acttctgtaa gggtgccact cggtacgatt aagagccgtt tggcgcgggc ccgattaaga
     3241 ttacgtgagt gtttacaggg cttttcggaa cttttacccg cttcatttcg tctggaagaa
     3301 gaaagtaacg cttaatgaat cgacgtgata ttgaacttct ttctgcatat ttggacggac
     3361 aactcaagcc gtctgacacg acgcgcctgg aatcccgtct caaatcggat cctgaactgg
     3421 ttgccgtcat ggacgatttg cgggcggctc gaaacctgct tcgcaaactc ccccagcgac
     3481 gcgcaccccg taactttacc ttgacccgca aaatggtggg ccaaaacccg ccgatgccgc
     3541 gggcgtatcc attttttcga tttgcaacca cgattgccac cctacttttg ttcttctcat
     3601 ttggattgaa cttcttagct ccccaaatgg cagcggcacc ctcgtttggt atgggcggtg
     3661 gcgggcctgg ccaagatctg ttttcatccg agcaggcaca agagccatca attgcagccg
     3721 ctccagtcga agcccccgcg gccacggagg cgccactacc gtcggccgaa atgctcccca
     3781 tgccgacgca aatatccacc ctggaatcag aacgcagtgc ggtgcctccc accgaaaaga
     3841 ttgacgcaac cggaaatgct gttgcatcag atcaatcgca ggtcgaaaac ccggtgccag
     3901 tgcctgtctc aactggctgg caaattgctt tagcagggat tgctgttgtt ggtgcacttt
     3961 tgatgttgtt actgcggcag tctgcattcc agcgctggcg tcaaaaagaa taattaaggc
     4021 gccaatgagg tgacggtcac tgtcccctca gctgtgcttc ggggatgctt cgcaaaagtg
     4081 actgtcacct cattggagac ttaattattc tttttgacgc cagcgctgga atgcagactg
     4141 ccgcagtaac aacatcaaaa gtgcaccaac aacagcaatc cct
//
LOCUS       DASTJP010000131         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1380327, whole genome shotgun sequence.
ACCESSION   DASTJP010000131 DASTJP010000000
VERSION     DASTJP010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380327"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFQ23_04030"
     CDS             complement(<1..366)
                     /locus_tag="VFQ23_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905776.1"
                     /translation="MIEIRSLTELNMASLERVAGGYASDSQYVVRYESAEDHISFDLQ
                     LVRLDQPYVKQFDYDDDTLQRYERLLNVGYSFGAYDEDRLVGLIIGEPDDWNRSLWVL
                     EFHVVDAYRKEGLGKQLMEC"
     gene            complement(511..1332)
                     /locus_tag="VFQ23_04035"
     CDS             complement(511..1332)
                     /locus_tag="VFQ23_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9905777.1"
                     /translation="MSPQNENTSTQQGKFIQANGIDMYYEDYGSGTPVVLLHGGLSTS
                     AMMKAFVPALAKHFRIITPDLRGHGKTNNPSSQFSYRLLAHDLAEFINRLELDQPFVC
                     GWSDGGQIALELGMQHPALAKGLLVGAAWFKFSESYQNFLRFLGLEAPGHVNYAQTEK
                     AVPQLVAALRSLHAPGPEYWKELLQQISTMWWTPLGYTAADFQRITIPTLILVGDRDP
                     VVPVEEAVEMYRLISKAELAIAAHTDHGFPRANPDTFTALTLSFLLRHSEIKDAG"
     gene            complement(1504..2298)
                     /locus_tag="VFQ23_04040"
     CDS             complement(1504..2298)
                     /locus_tag="VFQ23_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9905778.1"
                     /translation="MSYLAESYERFMVPSLFAPWATYLVQRANPQLGEHVLDLACGTG
                     IVARNVDPHVGSRGIVIGLDVNPDMIDMARVAAERDETAMEWHTGPAEQLPFPDENFD
                     LILCQFGLMFFTDRHAALKEMHRVLRKDGRVVLSVWQGLDRHPFYQTLHDVSLRHFGK
                     SSIEAVFSLGNSDKLHNLLTDSGFRQVMIEPVSITAYFPRPEEFLAWEIDINPAEAPA
                     LQNHDKEFQQAIIVAARQDMQSPLEEVTHEDQVVLQFHAHIAQARR"
BASE COUNT          658 a          671 c          659 g          622 t
ORIGIN      
        1 acattccatc agctgtttac caaggccttc cttgcggtag gcatctacga catgaaattc
       61 cagtacccat aaactgcgat tccaatcgtc gggctccccg atgataagac ctacaagtcg
      121 atcttcatca taggcgccaa aggaataccc cacgttgaga agcctttcat atcgctgcag
      181 tgtgtcgtca tcgtaatcaa actgcttgac gtacggttgg tcgaggcgta caagttgcag
      241 atcaaacgat atatgatctt ccgcactttc gtaccggaca acatactggc tgtcagaagc
      301 atagccacct gccacacgct cgagactggc catgttcagc tcggtcaaag atcgaatttc
      361 aatcattgca tgtactcaca tattcatcaa gcctgacggt tggcgttacg ggcggctggt
      421 ttagagacag gctcagaaac agaaacctcg ggaagtttag gaaaaagtcg ataaaacgcg
      481 gtgcatacca gctgtccagt gcacccattg ttagcctgca tcctttattt cgctatggcg
      541 caaaaggaag ctgagggtca gcgcggtgaa tgtatctgga tttgcgcgag gaaaaccatg
      601 atctgtgtgt gctgcaatcg ccagttctgc cttagagatg agacgataca tttcgactgc
      661 ttcttcaaca ggaacgaccg ggtctctatc gccgaccaaa atgagcgtag ggattgtaat
      721 tcgctgaaag tcagcggctg tgtagcccag gggtgtccac cacatcgtcg atatttgttg
      781 caacaactct ttccaatact cgggtccggg tgcatgaaga ctgcgcagcg ctgccacaag
      841 ttgggggaca gccttctcgg tttgcgcata attcacatga ccaggggctt caaggcccaa
      901 aaaacgcaag aagttttgat agctctcgga aaatttgaac caggcggcac ccaccaacaa
      961 accttttgcc agagcaggat gctgcattcc gagctccaac gcaatttgcc caccatcact
     1021 ccaaccacac acaaacggct gatcgagttc caagcggttt atgaactctg caagatcatg
     1081 agccaacagt cgataactga actgactgct tggattattt gtctttccgt gccctcgaag
     1141 atcgggcgta atgatgcgga agtgtttggc taacgctggc acgaacgctt tcatcattgc
     1201 cgaggtacta agtccgccat gcaggagcac gactggtgtt ccgctgccgt aatcttcgta
     1261 atacatgtca ataccgttgg cttggataaa ttttccttgc tgagttgatg tattttcatt
     1321 ttgcggagac attgtcaatt catcctattt tatttggcga atggcaggct aacggtctgc
     1381 attatcggca gctggggaga aaaaggacag gaaacgggga actgccatag ctcagaacca
     1441 gccccaaaaa gcggggacca ccgcccaatc cgctggctgt gttaggtggc attataagtc
     1501 gactcatctt ctggcctggg cgatatgcgc atggaattga agaacgactt gatcctcgtg
     1561 cgtgacttcc tccaacgggc tttgcatgtc ctggcgggcg gctacgatga ttgcttgctg
     1621 gaactcctta tcatgatttt gcagtgcggg agcctcggcg gggtttatat cgatttccca
     1681 ggcaaggaat tcttccggcc gtggaaagta cgccgtgatt gacacaggtt caatcatcac
     1741 ttgccgaaaa ccagagtctg tcaacaaatt gtgcagtttg tcggaattac cgagagaaaa
     1801 gacagcttcg atacttgatt tcccaaaatg ccgcaggctc acatcatgaa gcgtctggta
     1861 aaacggatga cgatccaatc cttgccatac actcaatacg acgcgtccat ccttcctcag
     1921 aacacgatgc atttccttaa gagctgcatg tctgtctgta aagaacatca acccaaactg
     1981 gcacaagatc aaatcaaagt tttcatctgg aaatgggagt tgctccgctg ggcctgtgtg
     2041 ccattccatt gcagtctcat cccgttccgc cgcgacgcgt gccatgtcaa tcatgtctgg
     2101 attgacatcg agaccaatga cgattccccg tgatccaaca tgtggatcaa cgttgcgagc
     2161 gacaatacct gtgccgcaag ctaaatctag gacatgctcg cccagttgcg gattcgcacg
     2221 ctgaacaaga taggttgccc acggtgcgaa taggctcggc accatgaagc gctcgtacga
     2281 ctctgctaaa taactcataa tatccaactt tcctgaggtt ttgccgccta atggtgagcg
     2341 ttacccgctt ggggtgggag cgtggactcg cccacgaaac ggaaaaaagc tcacagtcaa
     2401 gaaaacgctt gaaaacgcgg cgcgtacccc gagtcgcgtg cacgcattgt tactagaatg
     2461 tagcaggaag gggcgcggcg gagtatcctt ggagtgacaa acgcaccggg gatgctccgg
     2521 ggtgtgagca tcagggcaag ccgacaccgc ccatgagaga gaagctgtca cttctgcgct
     2581 cgtgttgtta cttggttgcc cggtggatcc
//
LOCUS       DASTJP010000132         3788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_390840, whole genome shotgun sequence.
ACCESSION   DASTJP010000132 DASTJP010000000
VERSION     DASTJP010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390840"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..687)
                     /locus_tag="VFQ23_04045"
     CDS             complement(202..687)
                     /locus_tag="VFQ23_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905779.1"
                     /translation="MLRWKKFDRSIIAGGMVLNQDFKEFVQSLNDNGVRYLVVGGYAV
                     ALHGYPRYTKDIDIWVEMTSANGSKILKALTQFGFGSLGVKESDFTVPNQMLQLGHPP
                     GRIDILTTLPGVEFSECYAARTTVKVDGVPVNFIDLENLRKNKKATGRHQDLADLENL
                     E"
     gene            complement(824..2146)
                     /locus_tag="VFQ23_04050"
     CDS             complement(824..2146)
                     /locus_tag="VFQ23_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9905780.1"
                     /translation="MKITLIEPAMIKSTNFSEKPSWQLTPLTLATLAGITPPDVEVEA
                     VDDRIEEIDYDTPRDLVGISVKTFTARRAYQIADEFRKRGVPVVLGGHHPTLLPEEAL
                     RFADSILIGEAEGIWPEVLRDAERKQLQRTYRQPHTLPFDGVKVNRSIFAGKKYLPVG
                     MVETTRGCPFSCNFCSVTTFFGRSFRHRPPAEVVAEIESLDKKFVFLVDDNIVADKEA
                     AKELFRALIPLKIRWISQASLTMTKDKELMRLMHQSGCAGVLTGIESISRANLKQIHK
                     SWNTVGIGYDEALKIARDNGIAIVGSFILGMDEDTPESLNELLDFAIRERFFAVLFNL
                     LTPYPGTELYKQFLQEGRLTKPQWWLDPEYTYGSAVFKPKNISAADLEQGRMRLYREF
                     YSPSSMLKRLWEPRANLQDLWHAFTFLTMNLPANKEEQMRFGKALGAC"
     gene            complement(2237..>3788)
                     /locus_tag="VFQ23_04055"
     CDS             complement(2237..>3788)
                     /locus_tag="VFQ23_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HET9905781.1"
                     /translation="ARDVEQAQNNVLSQYLADNRATEYGERYQFGDINSPSGYQERVP
                     LTTYDDYVEYANRIGAGSKSVLTNEPVRMFELSSGSTAASKLIPYTDRLKSEFQRGIA
                     PWIYNLYTNMPDLLSGPAYWSITPLMDGKRFTSSGIPIGFESDSEYLGVFGRWLADAV
                     MAVPNDVKTISDIDSFRYLTLLHLLRQPELRLISVWNPTFLSLLLAALPEWWDRLLMD
                     IKGGTVSIPEVSLQLAPKPELARMLRTISPMDYESIWPKLRLISCWMDGASASYATQL
                     AEMFPSVAFQGKGLLATEAFVSFPIVGVEGSVLAVNSHFFEFIDDSGDALLAHQIEKG
                     KTYSVVVTTGGGFHRYQLQDVIEVIGHWRQTPRIRFLGKADHISDWFGEKLEEQFVAN
                     VLRTVFSKHYLSPAFAMLAPDDSNGFRYLLFIESDESNENLAIDLDDGLRENFHYDYC
                     RRLGQLGETQIVQVIDGAETYLRACQARGQKLGNIKPGILQKTTGWISWFRDSDNKKQ
                     TRWNRKAR"
BASE COUNT          925 a         1005 c          893 g          965 t
ORIGIN      
        1 tttcattatg ggtgtttgga atactacctg tttattttca gccgttttag attagtgcac
       61 gttgtattgc caccagaggc tttgtccact tcatccccaa aggggatgct atagattcca
      121 cccacataca aagttttact accgcccggg ttgatcttct tgacaagatt gcagtcggca
      181 acagtcacgg tattgtttga actattccaa attttctaaa tccgcgaggt cttgatgcct
      241 acctgttgcc tttttattct tccgaagatt ctcaagatct ataaagttta cgggaactcc
      301 atctactttc acagttgtgc gagcagcgta gcattccgaa aactctaccc ctggcaaggt
      361 tgtcagaata tcaatgcgcc cgggcggatg tcctaactgc agcatctgat tggggacagt
      421 aaaatcggac tcctttactc ccaatgaacc gaacccaaac tgagtaagag ccttcaatat
      481 tttcgatcca ttcgcggagg tcatttccac ccaaatatcg atgtccttag tatagcgggg
      541 gtagccatgt agtgcgaccg cataccctcc aaccacaaga tagcgcacgc cgttatcgtt
      601 taacgattgc acaaactctt tgaagtcttg gttcagcacc atgcctccag cgatgatact
      661 cctgtcgaat ttcttccagc gcagcaagac gggcagaata tgtttgcctt ctccagtatg
      721 ccgcgtctgt cttcttgtcg gtcaattttc cttttcggac gacttttttc acaccagctg
      781 tttgtcgcat gacttaagta tactcgactc ttagcatgca cattcaacat gcgcctaaag
      841 cttttccaaa cctcatctgc tcttccttat tcgcaggtaa attcatcgtt agaaaagtaa
      901 aggcatgcca taaatcctgg aggttggcgc gtggttccca taaccgtttg agcatcgagg
      961 aggggctgta aaactcgcga tacaaacgca tccggccctg ctccaggtcc gccgcagaaa
     1021 tattcttcgg tttgaaaacg gctgaaccat atgtgtactc agggtccagc caccattgcg
     1081 gtttggtcag gcgtccctct tgaaggaatt gtttgtagag ttcagtcccg ggatagggcg
     1141 tcaggagatt gaacaacacg gcaaagaacc gttcccggat ggcaaaatcg agcaactcgt
     1201 tgagagattc cggcgtgtcc tcgtccatac caaggatgaa tgaaccgacg atcgcaatcc
     1261 cattgtcgcg ggcgatcttc aatgcctcgt cataacctat cccgacggtg ttccagcttt
     1321 tatgaatctg tttgaggttg gcccggctga tggattcgat tccggtcagc acaccggcgc
     1381 atccgctttg gtgcatgagg cgcatcaact ctttgtcttt cgtcatcgtc aggcttgcct
     1441 ggctgatcca gcggattttc aacgggatga gcgcgcggaa cagttccttc gccgcttcct
     1501 tatctgccac gatgttatcg tccacgagga agacaaattt cttgtccagg ctttcgattt
     1561 cagcaaccac ttcggcaggc ggacggtggc ggaaactgcg cccgaagaag gttgtcaccg
     1621 aacaaaaatt acaggagaac ggacagccac gcgtcgtttc caccattccc accggcagat
     1681 attttttgcc ggcaaaaatc gaacgattta ctttcacccc atcaaaaggc aatgtgtgag
     1741 gttgacgata agtccgttgg agttgtttcc gttccgcatc gcgcaggacc tcgggccaaa
     1801 tcccttccgc ttcgccgatc aggatcgaat cggcaaatcg cagcgcttcc tcaggcaaca
     1861 gcgtagggtg atgcccgccc aggaccacag gcacgccgcg cttccggaat tcatcagcaa
     1921 tttgataggc gcgtcgtgct gtgaaggtct tgactgaaat accgaccagg tcacgcggcg
     1981 tgtcgtaatc gatctcttca atgcggtcat cgactgcttc cacttcgaca tcaggcggcg
     2041 tgatccctgc aagagtcgca agagttaatg gggtaagctg ccacgaaggc ttttcgctga
     2101 agttcgtgct cttgatcatg gcgggttcaa tgagtgtaat tttcatattc gcttctaacc
     2161 gctacacctg ccctggtacg gcttaagcca cacggtgcca gggagcgcgc gacgaaaaaa
     2221 ttaaatcttt gggatcctag cgtgctttgc ggttccatct tgtctgtttc ttgttatctg
     2281 agtctctaaa ccaacttatc catcctgtag tcttctgtaa gatccctggt tttatgtttc
     2341 ccagtttttg accgcgtgcc tggcacgctc gcaaataagt ctccgcgcca tcaatgacct
     2401 gaacgatttg cgtctcacca agctgaccaa gcctccgaca ataatcgtag tggaagtttt
     2461 cgcgcaagcc gtcatcaagg tcaattgcca aattttcatt cgattcatca ctctcgataa
     2521 aaagcaggta gcgaaacccg tttgagtcat ccggcgcaag catcgcaaaa gccggagaaa
     2581 gataatgctt cgaaaatacc gttcttagaa cattcgcgac aaactgctct tccaattttt
     2641 cgccaaacca atctgaaata tggtccgcct tgccgagaaa gcgaatgcgt ggagtttgcc
     2701 tccagtgacc gatcacttca atcacatcct gaagctgata gcgatggaat ccaccacctg
     2761 tggtgactac cacagaatac gtcttgcctt tttcgatttg atgtgcgagc agggcatcgc
     2821 ccgaatcatc aatgaactcg aagaaatgtg aattgaccgc aagcacactt ccctctacac
     2881 cgacaatagg aaatgataca aaagcctcgg tggcaagcaa gccttttccc tgaaaagcta
     2941 cactggggaa catttctgcc agttgagtgg cataggatgc agaggcgcca tccatccagc
     3001 agctgatcaa gcgcagtttg ggccagatcg attcgtaatc catgggtgat attgtgcgta
     3061 gcatccgcgc caactctggt ttgggtgcca actgcaaact cacttccggt atcgagaccg
     3121 ttccgccttt gatatccatc agcaaacgat cccaccattc aggcagcgct gcgaggagca
     3181 gactcaagaa ggtcggattc caaaccgaga tcaaccgcag ctctggctgg cgcaagagat
     3241 gcaaaagcgt gagataacga aacgagtcga tatctgaaat agtctttaca tcattcggta
     3301 ctgccatcac tgcatcagcc agccaccgac caaagactcc gagatactcg ctatccgact
     3361 caaagccgat ggggataccc gatgacgtaa accgttttcc gtccatcaga ggcgtgatgg
     3421 accaataggc tgggccgctc agcaaatcgg gcatgttcgt atataggttg taaatccacg
     3481 gggcgatgcc ccgctggaat tcagacttca ggcgatcggt atagggaatg agtttggaag
     3541 ctgccgtgga gccgctcgaa agctcgaaca tgcgcacagg ctcattcgta agaacagact
     3601 tcgagccagc gccgattcgg tttgcatatt ccacataatc atcataggtc gtcagtggaa
     3661 cacgctcctg atatccggat ggtgagttaa tatcaccgaa ttgatatcgc tcgccatatt
     3721 ccgtggctcg gttatccgcc aaatattgag acaggacatt gttctgcgct tgttcgacat
     3781 ctctggct
//
LOCUS       DASTJP010000133         5063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1190790, whole genome shotgun sequence.
ACCESSION   DASTJP010000133 DASTJP010000000
VERSION     DASTJP010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190790"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFQ23_04060"
     CDS             <1..211
                     /locus_tag="VFQ23_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905782.1"
                     /translation="DSDLIRPEHTVEMFRLLGGGIFGDTPAGLPNSQLAILPGTSHVS
                     IVDRAELLVPMIASFLDAPMPKDGL"
     gene            218..826
                     /locus_tag="VFQ23_04065"
     CDS             218..826
                     /locus_tag="VFQ23_04065"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9905783.1"
                     /translation="MKNTEPAETDLPRSIGNPARRALEHAGYTKLKQLTKISEAELGQ
                     LHGVGPKALRILGETLEARGWSFKPAKAGEKLKKRGSPVSRTDKVDEFLRELRHPLKE
                     EVEAVRAIIKGVNKDINEEIKWKAPSFNYKGEYLVTFNLRETDRIHLVFHNPMISKVK
                     SKSLEGNYVDRRMLYLTDMKDIKSKQSELEKILKQLIKLNAH"
     gene            848..3097
                     /locus_tag="VFQ23_04070"
     CDS             848..3097
                     /locus_tag="VFQ23_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9905784.1"
                     /translation="MPQEYIEIRGARENNLKNVSLRIPKRKITIFTGVSGSGKSSIVF
                     DTIATESQRLLNENFSMFVRNFLPKYAQPDADAIENLSMSIVVDQKRMGGGSHSTVGT
                     ITDINTLLRLLFSRLGKPYVGPANLFGFNDPKGMCPECNGLGRKLDVDLDRFLDTSKS
                     LNDGAILFPEYAVKSWGWSLLLSTGLFDPDKKLSKYSAKEMDALLHHPPMKVKTNVGA
                     KDFNLTFEGIVDKFANKYIKRDLKTMSERTQKQVEPYMTMGPCTLCKGARLSQATLKS
                     KINGYNIADLTAMEITELIKVVKTFKDSSAQPIIKALIERLEHLVDIGLEYLSLSRET
                     DTLSGGESQRVKIVKHLGSSLTDVIYIFDEPSVGLHPRDVHRMNELLQRLRDKGNTVI
                     VVEHDPDVIKAADHIVDIGPQAGTHGGNIVYEGSFADLLKSNTLTGKHMKVKVPLKDE
                     FRKPTGKLPVKNAKVNNLQNVSVDIPTGVLTVVTGVAGSGKSSLINEVFLRQHPDAIV
                     IDQSAVGANSRSNPATYTGILDDVRKAFAAANKVNASLFSFNSKGACENCQGLGVIYT
                     DLAFLSEAKTPCEVCEGRRFKDEVLKYKLNGKNISEVLAMKVEDALEYFEIPEVTKKL
                     QAMSDVGLDYLSLGQPLSTLSGGECQRIKLASELHKKGSVYIMDEPTTGLHMSDIGHL
                     MEVINRLVDTGNTVVVIEHNLHVIKNADWIIDMGPEGGSKGGQVIFEGTPSQLVGVKR
                     SITSEYIQN"
     gene            3252..4706
                     /locus_tag="VFQ23_04075"
     CDS             3252..4706
                     /locus_tag="VFQ23_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9905785.1"
                     /translation="MRRIFLLLLGSLLFLVSCASQNQSTAPEAAIQLQDCTLISASGN
                     QVDARCGVLTVPEDRANPGGKQIELHVAVIPAIKRNPEPDPLFLLAGGPGQSAIETYS
                     SIIPLMFQIHESRDIILVDQRGTGKSNPLRCLTPEDDSLAEEEVFAKLKACPETLDAD
                     LGFYTTEIAMTDLDAVRSALGYEMINLYGASYGTRAALTYLRMFPEHVRTVTLDAVVD
                     SNFVMFMDAAEDGQAALDTFFERCEADEACQTTFPGLRNEFQSLLTRLEESPAEIIMS
                     HPLTNEPLELTVTREMITNMVFNTLYVPELVATMPLSIHAAYAKENYVPLISQAFLVD
                     AGLYDGMFYAVACTEDAPLISVDEANSRGEQTVFGGERPIDFADICSTWQKGEVSAEF
                     RQPVVSDVPVLMLSGSADPITPPYHADRVAESLTNEIHLVFTNTGHGNLTNTCGAKIF
                     QRFVETASIGGLETSCVENIQPPPFFIDFSGPRP"
     gene            4724..>5063
                     /locus_tag="VFQ23_04080"
     CDS             4724..>5063
                     /locus_tag="VFQ23_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9905786.1"
                     /translation="MIQVQGLSKSFGKIKAVQDVSFSASDGQITGLLGPNGAGKSTTL
                     RMLYTLLKPESGSAQIDGFDVRKSPLEVQRRIGVLADARGLYSRLTSRENVRYYGRLH
                     GLEGEALEKQI"
BASE COUNT         1406 a         1110 c         1283 g         1264 t
ORIGIN      
        1 cgattcggat ctcatccgcc cggagcacac cgtggaaatg tttcggctcc ttgggggagg
       61 catttttggc gatacgcccg ccgggttacc aaattcacaa ctggcaatcc tgccaggtac
      121 ttcacacgtt agcatcgtag accgcgccga gttacttgtt ccgatgatcg catcctttct
      181 cgatgctccc atgccaaaag atgggctata gatgaacatg aaaaataccg aaccagcgga
      241 aaccgacctc cctcgcagca ttggaaaccc cgcccgcaga gcgctggaac atgcagggta
      301 tacaaaactt aaacagttga ccaaaatctc tgaagcagag ctgggtcaac tgcatggagt
      361 gggtccaaaa gcattacgaa tattaggtga gacgcttgaa gcaaggggat ggtctttcaa
      421 gccagccaaa gccggtgaaa aattgaaaaa aaggggatcc cccgtcagcc gcaccgacaa
      481 agtggacgaa tttcttcgtg aactacgcca tccgctcaaa gaagaggtgg aggcggttcg
      541 cgctattatc aaaggtgtga ataaggatat caacgaagaa attaagtgga aggcaccttc
      601 cttcaattac aagggcgagt atttagtgac atttaatctg cgggaaacgg accgtattca
      661 tcttgtattt cataacccta tgatatcgaa agtaaagagc aaatctctcg aagggaatta
      721 tgtagaccgc cgcatgttgt atctcacgga catgaaggat attaagtcca aacagtccga
      781 actggaaaaa atcctcaagc agctgataaa attgaacgcc cattgacaca aaccttaagg
      841 agaaaacatg ccccaggaat acattgagat tcgcggcgca cgcgagaata atttaaagaa
      901 tgtttcactg cgaatcccga aacgcaagat tacaatattt accggcgtct ctggttcagg
      961 gaaatcctct attgtttttg atacgattgc aactgagtcg cagcgtcttt tgaatgagaa
     1021 tttcagtatg ttcgtgcgca attttttacc gaagtatgcc cagccagatg cagatgcaat
     1081 tgaaaacctg agtatgtcga ttgtggtcga tcaaaaacgg atgggtggcg gttcacactc
     1141 aacggtaggc acgatcaccg acatcaatac gcttctacgc ctgttgtttt ctcggctcgg
     1201 caaaccgtat gtggggcctg ccaatctttt tggttttaat gacccgaagg gaatgtgccc
     1261 tgaatgtaac ggattgggtc gtaaattgga cgtggacctg gaccgattcc tggatacatc
     1321 caaatcactg aatgacggtg caattctttt ccctgagtat gctgtaaaga gttggggctg
     1381 gagtttactc ctcagcacag gcttgtttga ccccgataag aaattatcca aatattcggc
     1441 gaaagaaatg gacgcgctcc tgcatcatcc gccgatgaag gttaagacga atgtcggcgc
     1501 aaaagatttc aacttaacct ttgaagggat cgtcgataag tttgcaaaca aatacatcaa
     1561 gcgcgattta aagacgatgt ccgaacggac tcaaaaacaa gtggaaccct atatgacaat
     1621 gggaccctgc actctctgta agggagcacg gctgagtcag gcaacgctga agagcaagat
     1681 caatggctat aacattgcag acttgactgc aatggaaatc acagaactga tcaaggttgt
     1741 aaaaacgttt aaggactcat ctgctcagcc gatcatcaaa gcattgatcg aacgcttgga
     1801 gcatttagta gacattgggc ttgaatatct cagcctgagc cgtgaaacgg atacgttatc
     1861 tggcggagaa tcacaacgtg taaaaattgt taaacatctg ggcagcagtc taacggatgt
     1921 gatttatatc ttcgatgaac caagcgtggg tttgcatccg cgtgatgtgc atcgtatgaa
     1981 tgaattgctg caacgattgc gtgataaagg caatacggtt atcgtggttg agcatgatcc
     2041 ggatgtcatc aaagccgccg accatattgt ggatatcggg ccccaggcag ggactcatgg
     2101 cgggaacatt gtctatgaag gtagctttgc agacttgctc aaatcaaaca ccttaaccgg
     2161 taaacatatg aaggtgaaag ttccattgaa ggatgagttt cgtaagccaa cgggtaaatt
     2221 gccagtcaaa aatgcgaaag taaataactt gcaaaatgtc agtgtagata ttccaacagg
     2281 agtgttgacg gtcgtcacag gtgttgcagg atcaggaaaa agctcgttga tcaacgaggt
     2341 cttcctaaga caacaccccg atgcgattgt gatagaccaa tccgcagtgg gagccaatag
     2401 ccgttcgaac cccgcaacct ataccggcat tctggacgat gtccgcaagg cttttgcagc
     2461 cgcgaacaag gtcaatgcct ccctgttcag tttcaattcc aagggtgcct gtgagaattg
     2521 tcagggattg ggtgtaatct atacagattt ggcgtttctc agcgaagcca agacgccctg
     2581 cgaagtttgt gagggcagac gcttcaagga cgaagtctta aaatacaaat tgaatggaaa
     2641 aaatatttca gaagtactgg caatgaaggt agaagatgcg cttgagtatt ttgagattcc
     2701 ggaagtcacg aaaaaactgc aggcgatgag cgacgtgggg ctggattacc tgagcctggg
     2761 tcagccgttg agtacattgt caggtggtga atgtcagcgc atcaagcttg ccagtgagtt
     2821 gcataaaaaa ggcagtgtgt atatcatgga cgaaccgact acgggtttgc acatgtctga
     2881 tatcggtcat ttgatggaag tgatcaatcg tctggtggat actggcaata ccgtggttgt
     2941 catcgaacac aatttgcatg tgatcaagaa tgccgattgg atcattgata tgggtccaga
     3001 gggcgggagt aagggtgggc aagtgatttt cgaaggtacg ccgagtcaac tggtcggcgt
     3061 gaagcgttcg attacgagcg aatacattca gaactaagaa aacctgatat gcagcaagct
     3121 tcgcgtacag cgcgggcagt taatcgttta tgctggtgaa acaaagaaga aatatctgtt
     3181 tgaggattga agacggtctc gaccaggcaa aaaatgatca aacgcattga gtacataaga
     3241 ccctttaagc catgagacga attttccttc ttttacttgg ttctcttctc tttttggttt
     3301 cctgcgcatc ccaaaaccag tcaactgctc ccgaggcagc catccaattg caagattgta
     3361 cgttaatctc agcttctggc aatcaggtag atgcacgttg tggcgtatta acagtccctg
     3421 aggatcgtgc aaaccccggc ggtaaacaaa tcgagttgca tgtagcggtt atccctgcca
     3481 tcaaacgcaa ccctgaaccg gacccgctct ttctgttggc aggcggaccg ggtcaatctg
     3541 caattgaaac gtattcctcc ataattccgt tgatgtttca aatccatgaa agccgcgata
     3601 ttatattggt cgatcagcgc ggcaccggca aatcgaatcc gttgcgctgt ctaacgccag
     3661 aggatgactc attagctgag gaagaggtct ttgcgaagct aaaggcctgt ccggagacct
     3721 tggatgctga tctgggattt tatacgaccg agatcgctat gactgatctg gatgcagtcc
     3781 ggtctgcatt gggatacgaa atgataaatc tctatggcgc ctcttatgga acgcgcgctg
     3841 cattgacata tctacgaatg tttcctgaac atgtgcggac tgttacgctc gatgccgtgg
     3901 tggattcgaa ttttgtcatg ttcatggatg cggcggagga tggtcaggca gcattggaca
     3961 cattcttcga acgttgtgag gcagatgaag cctgccagac aactttcccg gggttgagaa
     4021 atgagtttca gagtctgctt actcgtttgg aagagtcacc tgctgaaatc atcatgtctc
     4081 atccgctcac gaatgagccg cttgaattaa ctgtgacgcg tgagatgatt acgaatatgg
     4141 ttttcaatac gctctatgta cctgaactcg ttgcgaccat gcccttgtcc attcatgcgg
     4201 cgtatgctaa ggaaaactat gttccattaa tttcgcaggc atttcttgtc gacgccggat
     4261 tatatgatgg catgttttat gctgtagcct gcacagaaga cgcaccgctt atctctgtcg
     4321 atgaggctaa ctcacgaggc gagcagactg tcttcggcgg ggaacgccct atagattttg
     4381 ctgatatatg ttctacgtgg caaaagggag aagtgtcagc tgaatttcgt cagcccgttg
     4441 tatcagatgt tccagtctta atgctctccg gttcagctga cccgatcact ccaccgtatc
     4501 atgcagatag agtagcggaa agcctcacca atgaaatcca tttggtgttt accaatacgg
     4561 gacacggtaa cttaacaaat acgtgcgggg ccaagatttt tcaacgtttt gttgaaacgg
     4621 cctccattgg cggactcgaa acaagttgcg tggaaaatat ccaaccaccg ccgtttttta
     4681 ttgatttcag cggaccgcgt ccttagcgaa tcgagatatc actgtgatac aggtacaagg
     4741 actttctaaa tcatttggaa aaataaaagc agttcaggat gtatcttttt cggcgtcgga
     4801 tgggcaaatt acaggccttc ttggtccaaa cggagctggg aaaagcacca cgctacgcat
     4861 gctttatacg ttgctcaaac cggaaagtgg ttctgcacaa attgatgggt tcgatgtacg
     4921 caagtcgcca ctggaagttc aacgcagaat cggtgttctg gcagatgcgc gcgggttata
     4981 ttcacggctg acgagtcgtg aaaatgtccg ctactatggg cgattacacg gtctggaagg
     5041 ggaggcgctt gagaaacaaa ttg
//
LOCUS       DASTJP010000134         7575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1085474, whole genome shotgun sequence.
ACCESSION   DASTJP010000134 DASTJP010000000
VERSION     DASTJP010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085474"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFQ23_04085"
     CDS             <1..1021
                     /locus_tag="VFQ23_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905787.1"
                     /translation="NTPTATVTNTPTNTPTNTPTATATNTPTNTPTNTPTDTPTNTPT
                     NTPTDTPTNTPTNTPTDTPTNTPTNTPTNTPTDTPTNTPTNTPTNTPTNTATNTPTNT
                     PVREQGQLKVCKVAGLGVTPGTIFTINVNGVSYAVPAGRNGGLCVLAGQFPVNTNVTV
                     QEVIPAGYYVARIEVLPNNRLVSKDLTIGKAIVKVGTGVTEVIFTNKASGVPTPTAAP
                     ARPTRTPGAPTPTPAITGRLQICKEADGQGVTGNFTFTFAGKTRTIPVGACTDLIVVP
                     VGPLTITEVARTGFVVSDIYTIPADRLISENWGARTATVTIVQGNASTQTIVIFRNRV
                     DTIIR"
     gene            1406..4144
                     /locus_tag="VFQ23_04090"
     CDS             1406..4144
                     /locus_tag="VFQ23_04090"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HET9905788.1"
                     /translation="MQFQPTPYILPLILSTIIAVYVAIYVWQRRAIAPWAMALVVLAL
                     ACAEWSFGYALEIEGVDLAAKIFWGKIQYIGIVTVPLAWAIFTYSYTTQGIWMTRRNV
                     TLLSIVPFITLLLALTNEFHGLIWTNVQILTVGTFSALDVMYGFWFWVYWVYSNLLLL
                     AGTRAVFLSFKRTKGVFRRQDILLLIAVLTPWVGNVIYVSGLSPIPHLDITPFAFTIS
                     LVVFALGIYRFQLGTLAPVARDFVVEKMSDGMIVLDMQGNVVDINPSMQRALGVSAGE
                     VIRLIGQSSKDVFNAWPSLVERYENMAEAQDQISIGEDEAKEWFELNMSPLVDGRDRL
                     LGRVVTVRNITKRKQMEEALSLNEEKYRNIYENVEDVIYETDNQSHLTSISPSIKKRG
                     GYQPEELLGRSVTEFFVHPEQYADLDAQMIKNGSVNDFEALLRRKDGNTVWVSITSHI
                     VFDESGLPVSTEGVLRDISERKQAEDRIRQMNTELERHVNERTSQLQESNAYLTALIE
                     TSIALNESLDLNEVLDRILVQVHQLIPARAINVMLVEGEHAQIVRRVGYQGLENIAES
                     LRDYRFPITWPTLHYMFSTRKSLYISDTAGDSRWQNIQGADWVRSFVGAPLVVSDETI
                     GFLNAGHSEPNYLNAKHAFMLESLAHQASVAIHNARLLNELKKALEKEQGMRDQLVHA
                     DKLAALGKMVSVIAHEINNPIQTVKNTLYLLEDQIIPGSPAKDYLKMASAESKRISDL
                     VAQLRGTYASGSKAMVPVDVFALLLEVHDLLAPQLKERQVEWFQRNGHRPYTVLAVRN
                     NLKQVFINLCLNAMEAMEADQDRQLTVSLNLNQEGNQVGVGFHNTGTPISDDSLPHIF
                     EPFFTTKKSGTGLGLSISYDIIRHHNGELLVESGSGGVTFTVWLPLVASGYDGDEP"
     gene            4141..4944
                     /locus_tag="VFQ23_04095"
     CDS             4141..4944
                     /locus_tag="VFQ23_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9905789.1"
                     /translation="MTSQKTILIVDDEDPLRLSLSLILQKAEYLVETATNAEEALDHL
                     RLREFDLMFLDLNMPGMSGIDLLAEVHRYYPHMPVLILTAHAALESAIQAVRLGARDY
                     LIKPVEPVLILIRVAEIFAESEQPVRAKEIVSQLQSLVSELQLISGDQLTPAGLLEAV
                     PAADPARFLKKGNFELDLHARHVTLKGKYIPVAGFYFDYLGTLLRHAPKAVDYKVLVK
                     ESQGYEATGAEARDLARWRIHELRKMIEPDPQKPEYILTVRGTGYRLAI"
     gene            5155..5640
                     /locus_tag="VFQ23_04100"
     CDS             5155..5640
                     /locus_tag="VFQ23_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9905790.1"
                     /translation="MMDTLNILLVDDDQNLATTLSHGLRKALGKANSIIFCSSGPEAI
                     ALLATQPFDVVISDFNMPGLSGLDLLKKIRQDHAETSLVLITAFGTETLEEEVNRLGF
                     GYITKPFEMPILLQSIQGLIQNKATREGTKDAAHVSVLERKVGPSQSLLRTSSFSELQ
                     D"
     gene            5668..6450
                     /locus_tag="VFQ23_04105"
     CDS             5668..6450
                     /locus_tag="VFQ23_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9905791.1"
                     /translation="MHISENKTDDVRNESLTRNLFSEWIHASDNDIGSAPRIFVVCDQ
                     SDTAQVWSYVLSQQRLIVIAETSLEKATDAWCVEMPELGVIDINCEGQDLIALCKKFR
                     AVSVAPLLLFVPAYDEKQILEAYEAGIDEVVVKPVSPAMFLAKIVSWLRQRFRERLGE
                     ISSVGTHKYRLNIARRCLIDSHENEIKLTKLEFRLLTIVSSRIGQVFSDESLIREIWG
                     RYRPEDQVLLTNIIYRLRKKIEANPSQPRHLQRWQGGYSFHP"
     gene            6961..>7575
                     /locus_tag="VFQ23_04110"
     CDS             6961..>7575
                     /locus_tag="VFQ23_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905792.1"
                     /translation="MNKNIQTAVFKLMDIIVILTMVLSSPMSVFAAPLGQDSTPVLTA
                     NDADYETGESAHLTGSGFAPGPYTLAALGPDSVVVDWGTVNADSDGNFTADSPSLNAA
                     GSYVVSALAQGDNSTAATVTFTVTEAPAPTDVPTEEPTATEPPTAEPTQEPTATEPPP
                     ATESPTAEPTQEPTATEPPTAEPIQEPTQEPTLVPPPYIQSDKLD"
BASE COUNT         2000 a         1841 c         1886 g         1848 t
ORIGIN      
        1 caatacccca accgcgacgg tgacgaacac acctaccaac actcctacca acacgccaac
       61 cgcgacggca acgaataccc cgaccaatac gccaaccaac acacccacgg atactccgac
      121 caatacaccg acgaatacac caaccgatac tccgacaaac acaccgacca acacgccaac
      181 cgatactccc accaatacac cgaccaatac accaaccaac acaccaaccg atactccgac
      241 aaacacaccc accaatactc ctaccaacac gcccacgaat acagcgacga atacgccaac
      301 caataccccg gttcgggagc agggacaact caaggtttgt aaggttgcag ggctgggtgt
      361 cacaccggga acgatcttca cgatcaatgt gaacggtgtt tcctatgctg tgcccgccgg
      421 aagaaatggt ggcttgtgcg tgctggctgg gcagttcccc gtcaacacca acgtcactgt
      481 tcaggaagtg atcccggctg gctattacgt ggctcgcatc gaagtgctgc cgaataaccg
      541 actggtcagc aaagacctga caattggcaa agcgatcgtt aaggtcggta ccggagtcac
      601 cgaagtaatc ttcaccaaca aagcaagcgg tgtaccaacc ccgacggccg ccccagcaag
      661 acccactcgt acccccggtg ctcctacccc caccccagca atcacgggcc ggctgcagat
      721 ttgtaaagaa gcggacggtc agggtgtgac gggtaacttc actttcactt ttgcaggtaa
      781 gacccgcacc atcccagtgg gtgcctgtac cgacctgatc gtggtgccgg ttggaccatt
      841 gaccattacc gaagtggcac gtactggctt tgtggtatca gatatctata ccatcccggc
      901 agatcggttg attagcgaga attggggtgc gcgcactgca acggtcacta tcgtccaggg
      961 caatgcatct acccagacca tcgtcatctt ccgcaatcgc gtggacacga ttattagata
     1021 aagggttgag acaagaacga atgatcgccg gtgtcattct ctgtcatacg gatacacgga
     1081 gtggggcttc aatcaagtag acattttatc cagccggaaa ccaaacaagt cggagcctca
     1141 aggctccgac ttgtttttat ctggattgcg ttgaactctt cgaagattgc tctttgaaat
     1201 ctgatgagat accctcttct atttttgagg gtgcctttgt ataataacta tccaaaaagg
     1261 acttccgtct ctttgcattg cacggaggcg ccaataaaag cctttatcaa atttccaatg
     1321 ttgaagtccc tgtttcattt acaatagaaa aatcgaaagc aattcagggt ctgtgccgca
     1381 taaagcccgt atgtgagatg gtctaatgca atttcaaccc acaccctata ttctgcctct
     1441 catcctttca accattattg ctgtatacgt agcaatctat gtttggcagc ggcgcgccat
     1501 tgccccatgg gcaatggcgc ttgtcgtttt ggctctcgca tgtgctgaat ggtccttcgg
     1561 ctatgcactt gaaattgaag gtgttgatct agcagccaag atattctggg gaaaaattca
     1621 atacatcggc attgttacgg tgccgcttgc ctgggctatt ttcacgtact cctatacaac
     1681 ccagggcatc tggatgacgc gccgcaatgt gaccttgtta tccattgtgc cgtttattac
     1741 tcttcttctt gccctcacga acgaatttca tggtctcatt tggacgaatg tccaaattct
     1801 tactgtgggc acattttcag cgctggatgt aatgtacggg ttctggtttt gggtctactg
     1861 ggtgtattct aatcttctgc ttttggcagg gacgcgagcg gtttttcttt cattcaagcg
     1921 gacgaaaggt gtgttccgca ggcaggacat tctcctgctg attgcagtgc tgacaccctg
     1981 ggttggaaat gtgatttatg tatcaggttt gtctccaatc cctcatttgg atataacgcc
     2041 gtttgctttt acgattagcc ttgttgtttt cgctttgggt atttatcgtt tccagttggg
     2101 caccctggcg ccagtagcac gcgacttcgt tgtggagaaa atgtctgacg gaatgatcgt
     2161 actggatatg cagggaaatg ttgtggacat caatccgtcc atgcaaaggg cgctgggcgt
     2221 atcggctggc gaggtaatta ggctaattgg gcaaagctcg aaagatgtat ttaatgcctg
     2281 gccttccctg gtggagcgat atgaaaatat ggcggaggcg caagatcaga tctccattgg
     2341 tgaggatgaa gctaaggagt ggtttgaact gaacatgtct cccttagtcg atggacgtga
     2401 tcgcttactt gggcgtgtcg tgaccgtgcg caacatcacc aagcgcaaac agatggaaga
     2461 ggcgctgagt ctcaacgagg aaaagtaccg caacatctac gaaaacgttg aagatgtgat
     2521 ttatgagacg gacaatcaaa gtcatttgac gtccatcagt ccttctatta aaaagcgggg
     2581 aggctaccag ccagaagagc tgcttggacg cagcgtaact gaattttttg tccatccgga
     2641 acagtatgcg gatctggacg cacaaatgat caaaaatggc tcggtgaatg actttgaagc
     2701 cctcctccgg cgtaaagatg gaaacaccgt atgggtttcg attacgtcac acattgtatt
     2761 tgatgaaagt ggactgccgg tatccacaga aggcgtactg cgcgatatct ctgaacgaaa
     2821 acaggccgaa gaccgcatcc gacagatgaa tactgaattg gagagacacg ttaatgagcg
     2881 aacaagccaa ctacaggaga gcaatgcgta tctcaccgcg ctaatagaga catccatcgc
     2941 actgaatgaa agtctggatc tgaatgaagt ccttgatcgt attcttgtcc aggtccatca
     3001 attgattccg gcacgcgcta tcaacgttat gttagtggaa ggcgaacacg cccagattgt
     3061 ccgacgtgtg ggttatcagg gtcttgaaaa tatcgcagag agtttgaggg attaccgctt
     3121 cccaatcaca tggcccacat tgcactatat gttttctacc cgcaagagcc tctatatttc
     3181 tgatacggct ggagatagcc gctggcaaaa tattcagggt gcagactggg tgcgctcttt
     3241 tgtcggagct cctttagtgg tcagtgatga aaccattggc ttccttaatg ccgggcacag
     3301 cgaaccaaat tacctcaatg ccaagcacgc ctttatgctg gagtctctgg cacatcaggc
     3361 ctctgttgcc atccataacg cgcgtctctt aaacgaactg aaaaaggctc tcgaaaagga
     3421 acagggaatg cgagatcaac tggttcacgc cgataagctg gccgctctgg gaaaaatggt
     3481 atctgtgatt gcgcatgaga ttaacaaccc catccagacg gttaagaata cgttgtacct
     3541 tttggaagat caaatcatac cgggcagtcc agccaaagac tatctgaaaa tggcatcggc
     3601 agagtccaag cgaatttctg acctcgttgc tcagttgcgt gggacgtatg cctccggttc
     3661 gaaagcaatg gtgcctgtgg atgtcttcgc actgctcctg gaagtgcatg acctgcttgc
     3721 tccccaattg aaagaaagac aggtggaatg gttccagagg aatgggcacc ggccctacac
     3781 ggtgctggcg gtacgcaaca atctaaaaca ggttttcatc aacctatgcc tcaacgccat
     3841 ggaagcaatg gaagcggatc aagaccgcca gcttacggta agcctgaatt tgaatcagga
     3901 aggcaatcaa gtgggtgtgg gcttccataa tacaggcaca ccgatatcag atgactcctt
     3961 acctcatatc ttcgaaccat tttttacaac gaaaaagagc ggcaccggac tagggttatc
     4021 aatcagttac gatatcatcc gtcaccacaa tggtgagctt ctggtggaga gcggatctgg
     4081 aggcgtgacg tttacagtct ggctgccact tgtggccagt gggtacgatg gggatgagcc
     4141 atgacatcgc aaaaaaccat cctcattgta gatgatgaag atcctctgcg actcagcctg
     4201 tccttaatat tacagaaggc agaatacctc gttgaaaccg cgacgaatgc agaagaagcc
     4261 ttagaccacc tgaggctgcg agaattcgat ctgatgttcc ttgatttgaa catgccagga
     4321 atgagcggaa ttgatctgct ggccgaggta caccggtatt atccgcacat gcctgtgcta
     4381 attcttactg cacatgccgc cctggaatct gctattcaag cggttcgtct cggtgctcgc
     4441 gattatctaa tcaagcccgt cgagccagtg ttgatcctca tccgtgtcgc ggaaattttc
     4501 gccgaaagtg aacaacccgt ccgagccaag gaaattgtca gccagcttca gagtcttgtg
     4561 tctgaacttc aattgatcag tggagaccaa ttaacccccg ccggtctgct cgaggctgta
     4621 ccggctgccg acccagctcg ttttctgaaa aagggcaact tcgaactgga tttgcatgcc
     4681 cgtcatgtca cattaaaggg taaatacatt cctgttgcag ggttctattt tgattatctc
     4741 ggcaccctgc tacgtcacgc tccaaaagcg gttgattaca aggttctagt gaaagagtcc
     4801 cagggatatg aggcaacggg cgcggaagcc agggatctgg ctcgctggcg catccacgaa
     4861 ttgcgcaaga tgatcgaacc ggatcctcag aagcctgaat acatcctcac cgtacgaggc
     4921 accgggtatc gtctggcgat ctgagaaaga aaatggatat ttccacaaag ttcctcgaaa
     4981 actctcatga ttgagatgaa ttttgtgcat aaacttgacc gtatagaccg tcccgaaggt
     5041 ttgggcaaga aaaaggtggg gaaaaggaag gctctaattg acgagaagcc ttcgggaggg
     5101 tctgtgtatg gggattaaag caacctaatg tgaatcatga cgacaatggg gaatatgatg
     5161 gacaccttaa atatcctcct cgtagacgat gaccaaaacc ttgcgacaac cctctctcat
     5221 gggcttcgta aagctttggg gaaagcaaat tcgattattt tttgttccag cggacccgag
     5281 gcgattgcgt tactggcaac gcaacccttt gacgtggtga taagcgactt taatatgccg
     5341 ggcctatcag gtctggattt gctcaagaaa atcaggcagg atcatgctga gacaagcctt
     5401 gttctcatca cggcttttgg aacagaaaca cttgaggaag aggtgaatcg gcttggattt
     5461 ggctatatta ccaaaccgtt tgaaatgcca attctgctgc aatcgatcca aggtttgatt
     5521 cagaataaag cgacccgaga gggaacaaaa gacgcggcgc atgtcagtgt actggagagg
     5581 aaggtagggc cgagccagag cttgctgcgc acgtcctctt tcagtgaatt acaagattag
     5641 aaaggtttca gtattttggg gtgggccttg catatttcgg aaaacaaaac agacgatgta
     5701 cgtaacgaat ctctcacgag aaacctattc agtgagtgga ttcatgcatc tgacaacgac
     5761 ataggatccg cacccaggat ttttgtcgtc tgtgaccaaa gtgataccgc acaggtgtgg
     5821 agttatgtgc tctcacaaca gcggctgatt gtcatcgctg agacctctct ggaaaaggcg
     5881 acggatgctt ggtgtgtgga aatgccagaa ctgggcgtga ttgacatcaa ctgtgaaggt
     5941 caggatctca ttgcgctttg caaaaagttt cgggcagtga gcgtggcacc cctattgttg
     6001 tttgtgcctg cgtacgatga aaaacaaatc cttgaagctt atgaagcagg tatcgatgag
     6061 gtggtggtaa aacccgtcag cccggcgatg tttttagcca agatcgtgag ctggctgaga
     6121 caacgattta gggagcggct gggcgagata agctctgtcg gtacgcataa atacagattg
     6181 aatatagctc gacgatgctt gatcgattcg catgagaatg aaatcaaatt aaccaagctg
     6241 gaattccgtc tgctaacgat cgtgagcagt cgaattgggc aagtcttcag cgatgagagc
     6301 ctgatcagag aaatatgggg gaggtatagg cctgaggatc aggtattgct cacgaacatt
     6361 atttaccgtc tacgcaagaa aattgaagca aatccgagtc agccacgcca tttacaaaga
     6421 tggcaaggag gctattcctt tcacccttaa gtcgcatatt gagaaaatct gtttataact
     6481 tattactact ggttacgacc gttttacgat tcaattgcga ccctactatt tatgagcctg
     6541 catataatat gtcatacatt tgcggcgatg attcagttga cctttctttg gtcgcttgcg
     6601 cacacatcct gcggggtaac tgaggagcca atagcgaaac cgaaatcaac aggtgtagga
     6661 aatatgcata gttgaacgcg ttggaagcaa tcacaaactg ttttttgttt tgatgttcct
     6721 ttaaatcagg ttggaggaga atgcgtcatt ggcataacgg aacatattgt tcgcagaaga
     6781 tgactgaagg caaggatttg ctttcagcgt tttgcgcgtt cgtgggatat acctttaatt
     6841 cctgcgcttg tagcagtcag ccactaaatg agggcattta gaaccgacaa ccgtgtcggt
     6901 tcttattttt atcttttctt agaaatttag aaattcgtag ccaagtttga ggaggatcta
     6961 atgaataaaa atattcaaac cgcggtgttc aagctaatgg acatcattgt gattctgaca
     7021 atggtcttgt caagtccgat gagtgttttc gcggcgccgt tgggacaaga ctcaacaccc
     7081 gttctcacag cgaatgatgc ggattatgag acaggtgaat ctgcccatct cacgggatct
     7141 gggtttgcgc ctggtccgta tacgctcgct gcattagggc ccgatagcgt tgtcgtcgat
     7201 tggggaacgg ttaacgccga tagcgacgga aacttcactg ctgattcacc gtcgctcaat
     7261 gctgctggca gttatgtcgt gagtgccctc gcgcaaggcg acaactcgac agccgcgacc
     7321 gtgacgttta ccgttacaga ggctcctgca ccgacggatg ttcctacaga agagcccact
     7381 gccacagaac ctccgaccgc ggagccgact caggagccaa ccgcgactga acctccaccc
     7441 gctaccgagt ctccaaccgc ggaacccaca caagagccga ctgcgacgga acctccaacc
     7501 gcggaaccca ttcaggaacc gactcaggaa ccaacacttg ttcctcctcc atacattcaa
     7561 agtgacaaat tagat
//
LOCUS       DASTJP010000135         7681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_556598, whole genome shotgun sequence.
ACCESSION   DASTJP010000135 DASTJP010000000
VERSION     DASTJP010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556598"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2160)
                     /locus_tag="VFQ23_04115"
     CDS             complement(<1..2160)
                     /locus_tag="VFQ23_04115"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET9905793.1"
                     /translation="MKSSRTAVPLEKLMEQLPERYTITERELIQRAYRIAEEAHREQK
                     RHSGEPYINHCLAVASILADLRVPSEVVAAGLLHDTVEDTYITLKDLRRDFGDTIANL
                     VDGVTKLTNLPRVSRGDQHAERSNGNEPAESEAEDGPFGRKQDLISETLRKTFLAMGD
                     DIRVVLIKLADRLHNMRTLGFMPESKRKRIAKETLDIFAPLANRLGIWQIKWELEDLG
                     FRYLNPEKYKEIAEQLQEPRPDREIQIETIKDYLIKLLANNNIKAEISGRPKHIYSIY
                     KKMTQKGKPFDLVRDVRAVRLIVPDVPSCYAALGVIHTHWRPIPGEFDDYIAAPKDNF
                     YQSLHTAVIYDDKRPLEVQIRTHEMHQNAEYGIASHWRYKEGAKGSDKQYEERVNWMR
                     RMMEWRSDVSDAHEFVEGMKTDVFQDRVYVFTPRGDAIDLSAGSTPIDFAYHVHTDIG
                     HRCRGARINGKLSPLYQELKTGDQVEILTAKRGGPSRDWLNPNLGLVKTQRARSKIKA
                     WFKKQEDEQNLSQGRATLERELQRLGITELNFEKMARGLSFKIPDEMFIALGCGDLSV
                     NKVIRQISETEETADILEITTSTYGSKTSTDSVEVVGLKGLLSTMARCCNPMPGDPIV
                     GYITRGRGATVHRQDCPNVLRRKDKERLLQVGWGKVEQTYSVPVTVKAYDRQGLMGDI
                     STLLQNEGVNIADVSVNFNRSVADLKLVVEVRDLAQLS"
     gene            2262..2744
                     /locus_tag="VFQ23_04120"
     CDS             2262..2744
                     /locus_tag="VFQ23_04120"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9905794.1"
                     /translation="MAKVNNVTRLLDSRKISYTAFETPPDKLGALEVAGLLNISPDEV
                     FKTIVVTREKGKKPILAVLPGPRTVDLKLLAAFLGDKKVYLPTEREAEQLTGLQAGGI
                     SPLALINKGFQVVIDTLAQSHSEIHISGGERGLNIRLPVSDLINLTNARIAPISQSPR
                     "
     gene            2876..4048
                     /locus_tag="VFQ23_04125"
     CDS             2876..4048
                     /locus_tag="VFQ23_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9905795.1"
                     /translation="MRKITTLIGVLTLISVLLAACAPATTQAPAPTEPAATEMPMTEA
                     PATEAPATEAPATEAPTTAPTTAAPVAAGGERLQAIMDRGSLICGAHGTFQGFGFLES
                     TGAWTGFDVDFCRAWAAALFNDAEAIEFVALSAQDRFTALQSGQVDVLSRNSTWTFTR
                     DAELGLTFGPVTFYDGQAIMAKAEIVPDAAAGLAALEGANICVQSGTTTELNLADQFR
                     AAGVNYEPVVFEDQNAAFQAYEEGRCDAITSDRSQLATQGQAVLSNFADHVILDVVMS
                     KEPLAPAVAQGDERLADMVHWVVYGMIQAEELGITSANVDEFLTSEDPVVRRLLGVEG
                     ELGSKLGLSNDFIYRAIKLVGNYGEVFERNLAPYGLARGQNDLWTNGGLMYAVPFR"
     gene            4105..5271
                     /locus_tag="VFQ23_04130"
     CDS             4105..5271
                     /locus_tag="VFQ23_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9905796.1"
                     /translation="MPITAFWRNVRFLQALGQVVFVLLLALVAGILYANLSTNLARQG
                     LTVGYGFVNNPASFGIGESYISYEPSNSYGRAILVGLINTLVVSALGIILTTVVGVIA
                     GIARLSSNWLISKIAAAYVGVIRNTPLIIQLMFWYFGIILQLPSVRNAVALPGPSYLT
                     QRGVYLPGFESSPTFESWKIYIWMALASLIVVALLFRWLQTRSDLPTPLWWYSAYLLL
                     PALILWLGFIIQPEPPLIETIPELTGLNFRGGTRLTPEFAALLFGLVMYTGAFIAEIV
                     RAGIQAISRGQVEAARSLGLNSAQTLRLIVFPQAMRIMIPPVTSQYLNLAKNSSLAIA
                     IGYPDLFSIAGTVINQTGATIEVIVIMMLSYLSMSLFTSLLMNIYNSRVRLVER"
     gene            5273..6469
                     /locus_tag="VFQ23_04135"
     CDS             5273..6469
                     /locus_tag="VFQ23_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET9905797.1"
                     /translation="MTSMEMQPPPLSVGPIAWLRKNLFSTWYNVLLTFLASGLLYALL
                     RPAIQWSMTEARWGVIEANLTLFMIGQYPRDQLWRIWLTIYLLSALIGLSWGVWKDAA
                     RGFALIALGAGIAFILVGLMLNSEVWMNWLIAVSILLLSFALGRILPAQGPSFVTTAW
                     LLYFPLVFLLVAGAGFIPGLPVVNTNQWGGLLLTLMLTIVGNVGALPLGILLALGRRS
                     KLPILRYFSVGYIELIRGVPLVTILFMANLLVPLFLPVDIRPPDAVVRVMIGFIMFEA
                     AYQAENIRGGLQSINRGQYEAAQALGLNGALTMALIVLPQAIRAVIPALFNSFISLFK
                     DTSLVAIIGLFDILRIARSVLAQQEWLGTHREVFLFAFFIYWGFNLAFTYGSRRLEEA
                     MGVGKR"
     gene            6481..7236
                     /locus_tag="VFQ23_04140"
     CDS             6481..7236
                     /locus_tag="VFQ23_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET9905798.1"
                     /translation="MTEVEKDQEIIIARDVHKWFGNFHVLKGIDLTVQKGEVIVIFGP
                     SGSGKSTFIRCINRLEEHQKGQIIVDGVELTNDIRNIEAIRSEVGMVFQQFNLFPHLT
                     VIQNIMLAPSWVRRATKAQAHETAMQLLNRVGIPEQANKYPGQLSGGQQQRVAIARAL
                     AMQPKIMLFDEPTSALDPEMIKEVLDVMIQLAKSGMTMLVVTHEMGFARAVAKRMFFF
                     DEGQIVESGTPEEIFKSPKEDRTKLFLSQILSH"
     gene            complement(7273..>7681)
                     /locus_tag="VFQ23_04145"
     CDS             complement(7273..>7681)
                     /locus_tag="VFQ23_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905799.1"
                     /translation="CTTPPDTAVTSPPEDSVATNPSPENPFASQPEDDNRTRGEVFIH
                     EASLVIRESFPPQISVMLRGDLPTPCNALRAVINSPDEQNRIVVEVYSVVDPDRICTQ
                     VLQPFEESLALGTYPSGHYTVWVNDALAGEFDT"
BASE COUNT         1724 a         1926 c         1987 g         2044 t
ORIGIN      
        1 gctcaattga gccagatcac gaacttccac caccagtttc aaatcggcga cactacgatt
       61 gaaatttaca gagacgtccg cgatgttcac gccctcgttc tgaagcaacg tggaaatatc
      121 tcccattaac ccctgccggt cataagcctt cacagttacg ggaacggagt acgtttgctc
      181 gacttttccc cagcccactt gcagcagtcg ttccttgtct ttgcgacgaa ggacatttgg
      241 gcaatcctga cgatgaaccg tagcccctcg tccgcgcgta atataaccaa caattgggtc
      301 gccaggcatc ggattgcagc accgcgccat cgttgatagt aaccctttca agcctaccac
      361 ttctacggag tcagtagaag tctttgatcc gtatgtggac gtggtgatct caaggatatc
      421 ggcagtttct tccgtttcgg atatctgtcg gataacttta ttgaccgaaa ggtcgccgca
      481 gccaagggcg atgaacattt catcgggaat cttaaaactg agtccgcgcg ccattttttc
      541 aaagttgagc tcggtgatcc cgagtctttg caattcgcgt tccaaagtgg cgcgcccctg
      601 cgaaagattc tgttcatctt cctgtttttt aaaccatgcc ttgatcttcg aacgcgctcg
      661 ttgtgttttt accaaaccga gatttggatt cagccagtcg cggctgggtc cgcctcgctt
      721 tgccgtcagg atctcaacct ggtcacccgt tttcagctcc tgatagagag ggctaagttt
      781 tccgttgatg cgcgcccctc gacaacgatg accgatatcg gtgtggacgt gatacgcaaa
      841 gtcaatcggc gtggagccag cggagagatc gatcgcgtcg ccgcgtgggg tgaagacgta
      901 cacccggtcc tgaaatacat cagtcttcat tccctccaca aattcgtggg cgtcgcttac
      961 atcggagcgc cactccatca tcctgcgcat ccaattgacg cgttcctcgt actgtttatc
     1021 tgagcccttt gcaccttctt tgtaacgcca gtgtgacgca attccatatt ctgcgttttg
     1081 gtgcatttca tgcgttcgga tctgcacttc cagcggacgc ttatcatcat agatcacggc
     1141 cgtgtgcaat gattggtaaa agttatcctt cggcgctgca atgtaatcat caaactcacc
     1201 ggggatcggg cgccagtgcg tatgaatcac gcccaaggcg gcgtagcagg atgggacatc
     1261 cggcacaatc agacgcaccg cgcgtacatc ccggacgaga tcgaaaggct ttcccttttg
     1321 ggtcatcttc ttgtaaatgg aataaatgtg tttgggtcgt ccgctgattt ccgccttgat
     1381 gttgttgttg gcaagcaatt tgatgagata gtctttgatt gtctcaattt ggatttcgcg
     1441 atcagggcga ggctcctgta attgttccgc gatttccttg tatttttccg ggttgagata
     1501 tcgaaaaccg aggtcttcca attcccactt gatttgccag atgccgagcc ggttggcaag
     1561 gggagcaaag atatccaacg tctctttggc gatgcgcttg cgcttcgact cgggcatgaa
     1621 gccgagagtt cgcatattat gcaaacggtc cgccagtttg atgaggacca cgcgtatatc
     1681 atctcccatg gcgagaaagg ttttgcgcag ggtctctgag atcaaatcct gtttgcggcc
     1741 aaacgggcca tcctccgctt ccgactccgc gggttcgttg ccattggagc gttctgcatg
     1801 ttgatctccg cgcgaaactc ggggaaggtt cgttaatttc gtaacgccat ctacaagatt
     1861 tgcgatcgtg tcgccaaagt cccgcctcaa atccttgaga gtgatatagg tgtcttccac
     1921 ggtatcatgt aacaaaccag ctgcaacgac ctcagacgga accctcagat cggcgagaat
     1981 actggcaacc gccaaacaat ggttaatgta tggctcgccc gaatgacgct tttgctcccg
     2041 atgcgcctcc tccgcgatgc gataggcgcg ctgaatcagc tcacgctcgg tgattgtgta
     2101 tcgttcggga agttgttcca tcaacttctc gagagggacg gcggttctgg aagatttcac
     2161 aggatttatt gtactacgtg gtaacgaaat caaatgttaa cccgttaaaa tagaaacagc
     2221 ataaaatatt tgtcagatcg ctattaaagg tcaacttgag catggcaaaa gtcaataacg
     2281 ttacccgtct attagactcc cgcaagattt catacacagc ctttgaaacg cctcctgaca
     2341 agctgggtgc cctggaagtg gccggattac tcaatatttc gcccgatgaa gtttttaaaa
     2401 cgattgttgt cacccgtgaa aaaggcaaga agccaattct ggcagttctc cccggaccga
     2461 gaacggttga cctgaaattg cttgccgcct ttctcggcga taaaaaagtg tacctgccca
     2521 cggaacgcga ggcagaacag ctcacagggc ttcaagcggg cggcatctcg ccccttgcgt
     2581 taatcaataa gggctttcaa gttgtgattg atacgcttgc acaatctcac tcggagattc
     2641 atatttcagg tggagagcgc ggactcaaca tcaggctgcc tgtctctgac ctgatcaacc
     2701 tgacgaatgc tcgaatcgcc cccatatccc aatcacccag ataatctttt tcgctgaaat
     2761 tatttgctat aatgttcaaa ggcaaatgaa aggaggcaac acccaataaa gcttgaggtc
     2821 tcagaaaaat gttgaagtag gaaaaaatct aacactatct ctggaggagg agaatatgcg
     2881 taagattaca actctcatag gggttttgac tctcatcagt gttttgctag cagcctgtgc
     2941 accagcaaca actcaggcac cggctccgac tgagccggct gccacagaaa tgccaatgac
     3001 ggaggcgcct gctaccgaag ccccggcgac cgaagcaccc gctaccgaag cgcctacaac
     3061 tgcacccact acggcagcgc ccgtcgccgc cggaggggaa cgcctgcaag ctatcatgga
     3121 tcgcggaagc ctgatctgtg gagctcacgg caccttccag ggctttgggt ttttggagtc
     3181 cactggtgcg tggaccgggt ttgatgtaga tttctgtcgc gcctgggcag cggccttgtt
     3241 caatgatgcg gaagccatcg aatttgtcgc attgagcgca caggatcgct tcacagccct
     3301 tcagtctggt caggtggacg tcctgagccg caacagcacg tggacgttta cgcgcgatgc
     3361 cgaactggga cttacctttg gacctgtcac cttctatgat ggacaggcga ttatggccaa
     3421 ggcagaaatc gttccagatg cagcggctgg gctggctgcg ttggaaggtg cgaacatttg
     3481 tgtccagagc ggaacgacca ccgagttgaa tctagccgat caattccgtg ccgcgggcgt
     3541 caattatgag ccagtcgtgt ttgaagatca gaatgcggct ttccaggcct atgaggaagg
     3601 gcgctgcgat gctattacct ctgaccgttc tcagctggct acacagggac aggcagttct
     3661 ctcgaacttt gccgaccatg tcattctcga tgtggttatg tccaaggagc cgctggctcc
     3721 ggccgtggcg cagggtgatg agagattagc cgacatggtt cattgggttg tctatggcat
     3781 gatccaggct gaggaacttg ggattacctc tgcaaatgtg gatgaattcc tcacgagtga
     3841 ggatccggtg gtgaggcgtt tgctcggtgt ggaaggtgaa cttggctcga agctcggctt
     3901 gagcaacgac ttcatatatc gcgccatcaa attggtgggc aactacggtg aggtgttcga
     3961 aagaaacctg gccccctatg gtctggctcg aggccaaaac gatctgtgga ccaatggcgg
     4021 cttgatgtac gccgtaccgt tccgttaaaa atctaaagga tttgatttga gtggctcgca
     4081 aagtgagcca ctcaaataat cttcatgccg atcactgctt tctggcgtaa tgtccgcttt
     4141 ttgcaggcac tggggcaggt ggtgtttgtg cttctcctcg cgctggtcgc ggggattttg
     4201 tatgccaacc tctcaacaaa cctggctcgc caaggcctta ctgtaggtta tgggtttgta
     4261 aacaaccctg ccagttttgg tatcggtgag tcgtacatca gttatgaacc atcgaatagc
     4321 tatgggcgtg ctatcctcgt tggcttaatc aataccctgg tcgtcagcgc gcttggcatt
     4381 attctcacaa cagtcgttgg cgtgatcgcc ggaattgcgc gactttcttc caattggtta
     4441 attagcaaaa tcgcggctgc ttatgtgggg gtcatccgca atacgccttt gatcattcaa
     4501 ttgatgttct ggtattttgg aattatttta caactgccca gcgtgagaaa tgcagttgct
     4561 ttgcccggac cttcgtatct gacgcagcgt ggagtctatt tgcccggctt tgagtcctcg
     4621 cctacgtttg agtcttggaa aatctacatt tggatggcgc tggcgtcact gattgtggta
     4681 gcgttattat ttcgatggct tcaaacgcgt tctgacctgc cgactccgtt gtggtggtat
     4741 tctgcatacc tgctgttgcc agccttgatc ttatggctcg gttttatcat tcaaccagag
     4801 ccgccgttaa tcgaaacaat tcctgaactc accgggctaa attttagagg cgggacaagg
     4861 cttactccgg aatttgccgc tctcttattt ggacttgtca tgtatacggg agcattcatc
     4921 gctgagatcg ttcgcgccgg gattcaagct atctcgcgcg gccaggtgga agccgctcgt
     4981 tcattaggac tgaatagcgc ccaaacccta cgattgattg tgttcccgca ggccatgcga
     5041 atcatgatcc cgccagtgac aagtcagtat ctgaacctcg ctaaaaattc atcgctggcg
     5101 attgcgattg gttacccgga tttgttcagt attgccggta ccgtcattaa tcaaaccggt
     5161 gcgacgattg aagtcattgt gatcatgatg ctcagttatc ttagtatgag tctgtttact
     5221 tctttgctga tgaatattta taacagccga gtacgattgg tagagcgttg atatgacctc
     5281 gatggaaatg cagcctccgc cgctcagtgt cggacccatc gcatggctga ggaaaaacct
     5341 cttcagcaca tggtataacg tgctgcttac ctttcttgcg tcgggtctgt tgtacgcgct
     5401 attgcggcct gccattcaat ggtccatgac ggaagcccgc tggggtgtca ttgaagccaa
     5461 tttaactctc tttatgatcg gtcagtaccc gcgtgaccaa ctctggcgta tctggctgac
     5521 gatttatctc ctcagcgcat tgatcgggct ttcgtggggc gtctggaagg atgctgcccg
     5581 aggttttgca ttgattgctt taggtgcagg aattgcattc atcctggtcg gtttaatgct
     5641 caattcagag gtctggatga actggctgat cgcagtcagt atcctcctgt tgtcctttgc
     5701 ccttgggcgg attctcccag cacagggtcc ctccttcgtg accaccgcct ggctgctgta
     5761 ctttccattg gttttcctgc tggtagcggg cgccggcttc atacccgggc ttccagtagt
     5821 taacacaaac cagtggggag gtcttctgct taccctgatg ctgacgattg tcggtaacgt
     5881 cggggctctt ccacttggca ttttattggc gcttgggcgg cgcagtaaac ttccaatcct
     5941 tcgatatttc agtgttggct atattgagtt aattcggggc gttccactcg tgacaattct
     6001 cttcatggca aaccttcttg tgcctctttt tcttcccgta gatattcgtc ccccggatgc
     6061 ggtcgttcgt gtcatgatcg ggttcatcat gttcgaggca gcctatcagg cggagaatat
     6121 tcgtggtggc ctccaatcca tcaatcgcgg acagtatgaa gcggcgcagg ccctgggact
     6181 gaatggtgcc ttgacaatgg cattgatcgt cctgccgcag gcgatccggg cggtgattcc
     6241 agcattattc aattccttta ttagcttatt caaagatacc agtctggtag cgatcatcgg
     6301 actcttcgat attttgcgga tcgcccgttc cgtattggcg caacaggaat ggctgggtac
     6361 ccatcgcgaa gtttttctgt ttgcattttt tatctattgg ggattcaatc tcgcgtttac
     6421 ctacggtagc cgacgcctcg aagaggcgat gggtgtgggt aaacgctaat tggagacgtt
     6481 atgactgaag ttgagaaaga tcaggaaatt attattgccc gcgatgtgca taagtggttt
     6541 ggtaatttcc atgtactaaa aggcattgac ctgacggtcc aaaagggaga agtcatcgtg
     6601 atcttcggtc catccgggtc ggggaagtcg acgtttattc gctgtatcaa tcgtttggag
     6661 gagcatcaaa agggccagat cattgtggat ggcgtggaat tgacgaatga cattcgcaac
     6721 atcgaagcga tacgttcgga agtcggcatg gtatttcaac agttcaattt atttccacat
     6781 ttgaccgtga ttcaaaatat catgctggcg ccctcgtggg ttcgacgtgc taccaaagcc
     6841 caggcacatg aaactgccat gcagctgctg aaccgtgttg gcattccgga acaagcgaat
     6901 aagtatcctg gacaattatc aggtggacag caacaacgcg tagccattgc gcgggccctg
     6961 gcgatgcaac ccaagatcat gcttttcgac gaaccgacct ccgcgcttga ccctgagatg
     7021 atcaaagagg tgctggatgt aatgattcaa ctcgcgaaat ctggcatgac gatgcttgtg
     7081 gtcacacacg agatgggatt cgcccgcgcc gttgccaaac gaatgttttt cttcgatgag
     7141 ggtcagatcg tagagagcgg aactcctgaa gagatcttca aaagtccgaa agaggatcga
     7201 acaaagttat ttctttcgca gattctttcg cactagaaaa agagccggca cataccggct
     7261 ctttttgatt aactatgtat cgaactcgcc agccaatgca tcattgaccc acacagtgta
     7321 atgtccgctg ggataggttc caagagccag actttcctcg aagggttgca atacttgtgt
     7381 gcaaatccga tccgggtcaa ctaccgaata tacttctaca acaatcctgt tttgttcatc
     7441 aggagaattg atcacggccc tcagcgcatt gcatggcgtt ggcagatctc cgcgaagcat
     7501 gacggaaatt tgtggcggga aactttcgcg aatcaccagg ctcgcttcat ggatgaaaac
     7561 ttcaccgcgg gttcgattat catcttcagg ctgagacgca aaaggatttt caggtgacgg
     7621 atttgttgcc actgagtcct ccggtggact tgttacggct gtatcgggtg gtgttgtaca
     7681 a
//
LOCUS       DASTJP010000136         3223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_616696, whole genome shotgun sequence.
ACCESSION   DASTJP010000136 DASTJP010000000
VERSION     DASTJP010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616696"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFQ23_04150"
     CDS             complement(<1..389)
                     /locus_tag="VFQ23_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905800.1"
                     /translation="MKSISRTEFANLLVWLAVAVGAFMRLNPTLLAGFAINDGGMFAA
                     MVDDLKTSQYALPVFTTYNHLNIPYAYPPLGFYIGRIASDLLGFSAPETVRWVPAFFA
                     SLSVPVFYLVALQLLRSKYQAAISTLF"
     gene            complement(454..1770)
                     /locus_tag="VFQ23_04155"
     CDS             complement(454..1770)
                     /locus_tag="VFQ23_04155"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET9905801.1"
                     /translation="MKYSLKSSFARWVLVLFFGTFVSTVAGQLVAMTGASIFCQGFPL
                     CVPDHTLGWLKLAHVLSVGVAAILMLVVFHKAWIEQRAQRILLPLTTVLTVMFFGQAL
                     VGATQVMQGFPAHLRFLHQLTTVSLWVSLLLLVYTSGVLPASTNRLENLGKQQRARDF
                     FALSKPLIVGLLLITTYGGLVIGGKEWPSFSLTLWTLIGGALAAGGSSALNQYIDREL
                     DKNMQRTAKRPLADGRLMDAEGLAFGLGLSLISYYILACFVNGLAALLSLAGIIYYVV
                     LYSLWLKKATVQNIVIGGGAGAIPPMVGYAAATGHLDWTAWILFAIIFMWTPPHFWAL
                     AIVRMKDYKNAGVPMLPVVRGELETRRQIFVYTIELVLVTLLLPVLNLAGHVYLVSSL
                     VLGAALLYAAWAVWKQGGNKVAWRMYKWSSTYLVFIFLAIMIDSVL"
     gene            complement(1767..2510)
                     /locus_tag="VFQ23_04160"
     CDS             complement(1767..2510)
                     /locus_tag="VFQ23_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HET9905802.1"
                     /translation="MLLLKMVQPKWLLATLLVLLGTAFCIRLGIWQLDRLDGRRAFNK
                     QVETMRTLPALNLNQEAFDAIDEMEWRAVTVSGTYDFEHQIAIRNQYHDNQYGYHLLT
                     PLRFNETAVLVDRGWIPADSNSAPADWRKFDETGEVIVTGQLRLGAAKPAFGGVEDVM
                     PENGSRLDVWNNADITRIASQIPYPILPVYIQPNPQVEDVEPPIPFQPQIELTEGPHI
                     GYALQWFTFATILFFGYPFYIRKQEESSP"
     gene            complement(2497..2994)
                     /locus_tag="VFQ23_04165"
     CDS             complement(2497..2994)
                     /locus_tag="VFQ23_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905803.1"
                     /translation="MPTSTAVKRIMFALLIGLLGGVLISEIPFLFLQETARPPQEILL
                     TIPAGAAEQVARGEQPPSIPNNMSFVVGDVLVVENEDNVDHKLGSLWIPANSSARLPF
                     NREESLAFECTFQSDNYLGLDVHQPLTFGTRIYGIISVGLPLAILVALYSLVMPISKK
                     ENAVA"
     gene            complement(3014..>3223)
                     /locus_tag="VFQ23_04170"
     CDS             complement(3014..>3223)
                     /locus_tag="VFQ23_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905804.1"
                     /translation="LSGTVSELVPVWQKYGVFREIVDGNSAAGYIVNHTARVTLIDSE
                     GNMRVSFAFETPVQDIVHDLKLLLK"
BASE COUNT          929 a          816 c          776 g          702 t
ORIGIN      
        1 aagaacagtg tcgagatggc cgcctggtat ttactcctca gtaattgcaa agcaacgaga
       61 taaaatacgg gcactgacag tgatgcaaag aacgctggca cccatcttac agtttcagga
      121 gcgctgaaac ccaataggtc ggaggcaatt cttccgatgt aaaaccccag cggaggatat
      181 gcataaggga tattaaggtg attgtaggtg gtgaacacgg gcagggcata ttggctggtc
      241 ttcagatcgt ccaccatagc tgcgaacata cccccgtcgt tgatcgcaaa acccgcgagc
      301 agagtcgggt tgagacgcat gaaagcacca acggcgacag ccagccaaac aagtaggttt
      361 gcaaactctg tgcggctaat tgatttcatg caagcacttt atgaaattaa tggtatcaag
      421 aaaaaatttc tgttttatga agctaatgac ccactacagc acagaatcaa tcatgattgc
      481 caaaaagata aataccaaat aggtgctcga ccatttatac atgcgccagg caactttatt
      541 gccaccctgt ttccacacag cccacgcggc ataaagcaat gctgcaccca atacaagcga
      601 ggaaacaaga tatacatgcc cggccaggtt gagaacaggg agaagtaagg tgaccaacac
      661 caattcaatg gtgtaaacga aaatttgtct gcgagtctcc aactctccac gcaccactgg
      721 cagcatggga acgcctgcat ttttataatc cttcatccga acaattgcca aagcccagaa
      781 gtgtggcggc gtccacatga agataattgc gaagaggatc caggcggtcc aatcgaggtg
      841 acccgttgcg gcggcgtatc caaccatggg agggattgcc cccgcgcctc cgccaatgac
      901 gatattttgc acagtcgctt tcttgagcca gagactatat agaaccacat aataaataat
      961 cccggccagc gaaagaagcg cggcaagtcc gttcacaaaa catgcgagaa tataataact
     1021 gatcaacgac aaacctaatc caaaggccag tccttcagca tccatcaatc gtccatcggc
     1081 aagcgggcgc ttcgccgtcc gttgcatgtt cttatccagc tcgcgatcaa tatactgatt
     1141 caacgcactt gagcccccgg ctgccaacgc gcctccgatt aaggtccaaa gcgtgagcga
     1201 aaaggacggc cattccttcc cgccaatcac cagtccgcca taggtggtga tcaataacaa
     1261 tccaacgatc aacggtttcg ataatgcaaa gaagtcacgc gcacgctgtt gcttgcccag
     1321 gttttcaagt cggtttgtgc tggctggaag gacacccgag gtataaacca acagcaacaa
     1381 tgaaacccat aaagaaaccg tagtaagttg gtgcaaaaag cggaggtgcg caggaaagcc
     1441 ctgcatcacc tgcgtagcgc ccacaagcgc ctggccaaag aacattacgg tgagaaccgt
     1501 cgtcaatggc aatagaattc gctgggcgcg ttgctcgatc caggctttgt gaaaaacgac
     1561 gagcatcagg attgcagcaa cgcctacaga caaaacgtga gccagtttca accagcccaa
     1621 tgtatgatcc ggaacacata aaggaaaacc ctggcagaat atggaagccc cagtcatcgc
     1681 gacgagttgg cctgccaccg tggatacgaa tgttccgaag aataaaacca gtacccaacg
     1741 agcaaaagag gacttgagtg aatatttcat ggcgatgatt cttcctgttt gcgaatatag
     1801 aacggatatc cgaaaaataa gatagttgcg aaggtgaacc attgcaaagc atacccaata
     1861 tggggccctt ctgtcaattc aatttgcggc tgaaatggaa tgggaggctc cacatcttcg
     1921 acctgtggat ttggctggat gtagacaggc aaaatggggt aaggaatctg gctagcaatc
     1981 cgcgtaatat ctgcattatt ccagacatcc aggcgcgagc cattttcagg catcacatct
     2041 tcgacaccgc cgaaggccgg tttcgcagcg ccgagccgta actgacctgt gacgattact
     2101 tctccggttt cgtcaaactt gcgccaatct gcgggtgcgg aattgctatc ggcggggatc
     2161 cagccgcggt ctactaaaac cgctgtctca ttgaaacgaa gcggtgtcaa gaggtggtat
     2221 ccgtattgat tatcatgata ctgatttcgt atcgcgattt gatgctcgaa gtcatatgtt
     2281 ccactgactg tcacagcgcg ccattccatc tcatcaatgg catcgaaggc ttcctgattc
     2341 aaattcagag caggcagcgt gcgcatggtt tccacttgtt tattgaaggc ccgccgtcca
     2401 tcgaggcgat ctaattgcca gatgccaagt cggatgcaaa aggccgtgcc aagcaaaacc
     2461 agcaatgtcg ccagcagcca cttgggctga accatcttaa gcaacagcat tttctttttt
     2521 actgataggc atcaccaggc tatatagcgc caccaggatt gcaagaggaa gcccaacaga
     2581 gatgatgccg tagattcgag tcccaaaggt gagtggctgg tgcacgtcga gacccagata
     2641 attatcagac tgaaaggtac attcaaacgc aaggctttcc tcacggttga atggcaatcg
     2701 ggcagaggaa ttcgccggaa tccataatga tcccagtttg tggtccacgt tatcttcatt
     2761 ttctacaact aaaacgtcac caaccacgaa tgacatatta ttcggaatgg acggaggctg
     2821 ctcgccgcga gcaacctgct cggcagcacc agcagggatg gtgaggagga tttcctgagg
     2881 cgggcgggcg gtttcctgaa gaaacagaaa cggaatttca ctgatcagca cgcctcccag
     2941 caaaccgatc aaaagagcaa acataattcg cttcacagca gtcgaggtag gcattttcta
     3001 tcgtttcaca aaatcatttc aatagtaatt taaggtcatg gacaatgtct tgcacgggcg
     3061 tttcaaacgc aaatgaaacc cgcatatttc cctccgagtc gatcaatgta acgcgcgcgg
     3121 tgtgatttac gatataaccg gctgctgagt ttccatctac gatttcgcga aaaacaccat
     3181 acttttgcca aacgggcact agttcgctta cggttccact gag
//
LOCUS       DASTJP010000137         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_979215, whole genome shotgun sequence.
ACCESSION   DASTJP010000137 DASTJP010000000
VERSION     DASTJP010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979215"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFQ23_04175"
     CDS             <1..565
                     /locus_tag="VFQ23_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905805.1"
                     /translation="QSAVDMAEELNEPVVLSRALGALCSVLDGRSLLSEHLQFALRRL
                     EVSQDPRVDNPIERIDAISNAGMALMYVGEYEQAIPHLQEAETLASRAQAIGQQTAAI
                     GLQSQCALRLDRWDEVIVLEDKWRDLERRFPRHRVGPTCFNVAMSASIYALRGDLEKS
                     RAYGKESVDYMISMSGNPEIWQRNQFY"
     gene            617..1069
                     /locus_tag="VFQ23_04180"
     CDS             617..1069
                     /locus_tag="VFQ23_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905806.1"
                     /translation="MEHVIETAGQPVNFGTMAHDHEIYMTLADTAVELRDVDALRKYA
                     PNLEELAVRDNHKLYLAIAHRALGVERRLAGKHAEAEDRLRQALGLFTKLGARWQIGR
                     TLFELGELNLSHAPVKASEYYSQALGSFEEVQAKPYAERTRAALQQVG"
     gene            1080..2102
                     /locus_tag="VFQ23_04185"
     CDS             1080..2102
                     /locus_tag="VFQ23_04185"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9905807.1"
                     /translation="MKQIQTDEQAEHLASALAGDQGAFEKLVEPYRREILVHCYRILG
                     SFEDAEDISQEILLRVWKRLESFEGRSSLRAWLYKIATNACLDALDSRRARGLSKELY
                     PRGDPTGELPSPSNEVIWVEPFPDEYIDGQPNIYPEARYEVRESITLAFVAALQKLPG
                     RQRAALLLCDVLGWSANEAAEILDSSTAAVNSALQRARETMKQPERKPISASLNEQLS
                     ALLSRYVSAWEAADSAALVAVLREDVALTMPPLPVWFGGRDNVYRFIEEKLFKMFDPF
                     RVRLIPIRANSSPAFAAYQMDSNGDYRAAAIHILTIENGEITEINDFLTFDGQLFSKF
                     GLPLMI"
     gene            2186..2875
                     /locus_tag="VFQ23_04190"
     CDS             2186..2875
                     /locus_tag="VFQ23_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905808.1"
                     /translation="MNDQLTLYLLTIRGTLSPSTLEEARNIHNMTAGNPDGVAAAKSL
                     GDVSHMVYVPMPHNGHVKADGAGEFLIMDLWHSIDGLNTFFADKHVQEGGNMIFSQRD
                     PVVWAPAEGFTSFHIPAPSGNNDRVIAVVRGTVNSKEEAKKLHNAVMSKTISKARSLG
                     LLSHESYFRLAAPDSPEAMEYLGVDVWMNGERLGEYYGDEEFLAGYDGFFTAEATDSV
                     WVHPKGEWTEW"
     gene            complement(2940..>3256)
                     /locus_tag="VFQ23_04195"
     CDS             complement(2940..>3256)
                     /locus_tag="VFQ23_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905809.1"
                     /translation="RVIAVVRGTVNSKEEAKKLHNAVMSKTISKARSLGLLSHESYFR
                     LAAPDSPEAMEYLGVDVWMNGERLGEYYGDEEFLAGYDGFFTAEATDSVWVHPKGEWT
                     EW"
BASE COUNT          784 a          840 c          879 g          753 t
ORIGIN      
        1 acaaagcgca gtggatatgg cagaggaact gaacgaaccc gtcgttttgt cacgtgcgct
       61 gggcgcgctg tgcagtgtgc tggatgggcg tagtttactg agcgaacatt tacagttcgc
      121 attgaggcgg ctggaagtga gccaggaccc tcgtgtggat aaccccattg aacgcattga
      181 tgcaatcagc aacgcaggga tggcgctcat gtatgtaggt gaatacgagc aggctattcc
      241 acacttgcag gaagcggaaa ccttagccag tcgcgcgcag gcgattggtc agcaaacggc
      301 cgcgattggg ctgcaaagtc aatgcgcgct gcgtcttgat cgctgggatg aggtcatcgt
      361 actggaagac aaatggcgcg accttgaacg acgttttcca cgacatcgcg tgggtcctac
      421 gtgtttcaac gtggcaatga gtgccagcat atacgcttta cgaggcgatc tcgaaaaatc
      481 aagagcctac ggtaaagagt cagtcgatta tatgatctcc atgtcgggaa atcccgagat
      541 ctggcaacgg aaccagttct actgacaatc tctgccgctt aaggcgcagg gcgagttcgc
      601 tctggtgaaa cagaatgtgg aacatgttat cgaaacggcc gggcaacctg tcaattttgg
      661 gacgatggcc catgaccatg aaatttatat gaccctggct gacaccgcag tggaattacg
      721 cgatgtggac gcgttgcgca aatacgctcc caatcttgaa gaattagccg tccgcgacaa
      781 tcacaaactt tatctggcaa ttgcgcaccg tgcattgggg gttgagcgtc ggttggcagg
      841 taaacatgcg gaagcagagg atcgcctcag gcaggcgctt ggacttttta ccaagctggg
      901 cgcccgctgg cagatcggac gaaccctctt tgaacttgga gagttgaatc tcagtcatgc
      961 acctgtgaaa gcgagcgagt attattcgca ggctctcggc tcttttgaag aggttcaggc
     1021 aaagccgtac gcagaacgga cacgcgcggc gttgcaacaa gtaggatagg tgaagaatca
     1081 tgaaacaaat ccaaacggat gaacaggcag aacacttagc ctccgcctta gccggtgacc
     1141 agggtgcctt tgaaaaactg gttgaaccct atcgccgcga gatcctggtc cattgctatc
     1201 gcatcctcgg ctcgttcgaa gatgccgaag acatctcaca ggagatcctt ctgcgcgtat
     1261 ggaagcgtct agaatcgttc gaaggccgtt cctccctgcg agcctggcta tataaaatcg
     1321 cgacgaacgc gtgcctggat gcactggata gccgccgcgc ccgcgggctt tcaaaggagt
     1381 tgtatccgcg cggcgacccg acgggtgagc tgccctcgcc ttcgaatgaa gtgatctggg
     1441 ttgaaccctt tcctgatgaa tacatcgacg ggcagccgaa tatttatccg gaagctcgtt
     1501 atgaggtgcg cgaaagcatc acgctggcgt ttgtcgctgc gctgcaaaaa cttccgggtc
     1561 gtcagcgcgc agcattgctg ctctgtgatg tgcttggctg gagcgcgaac gaagctgcgg
     1621 agatcctcga ttcctccact gccgcggtca acagtgcatt gcagcgcgcc cgcgagacaa
     1681 tgaaacagcc cgaacgaaaa cctatctctg caagtttgaa cgaacagctt tccgccctgc
     1741 tgtcgcgtta cgtctccgcc tgggaagcag ccgactccgc cgccttagtg gcagtcctgc
     1801 gcgaagatgt tgctttgaca atgccgccct tgcctgtctg gttcggcggt cgcgacaatg
     1861 tatacaggtt catcgaagag aaactattca aaatgttcga tccatttcga gtgcgactca
     1921 tcccgattcg cgccaacagc tcgcccgctt ttgcagccta tcaaatggat tcaaacggag
     1981 attatcgagc tgctgcaatt cacatcctga cgattgaaaa tggcgaaatt actgagatca
     2041 atgacttcct gaccttcgat ggtcagctct tttccaaatt cggcttacct ctcatgattt
     2101 gaccgatgag ttcggcagag tcctcccgtc ttaataagtg aagaattaat cagacggact
     2161 ctgtccgact caaaaggaga aaatcatgaa tgaccaatta accctttatt tgcttacaat
     2221 tcgcgggacg ctctcacctt ctacattaga ggaagcgcgc aacatccata atatgactgc
     2281 cggtaatccc gatggtgttg cggccgcaaa atccctgggg gatgtcagtc atatggtcta
     2341 cgttccgatg ccgcataacg gccatgtgaa agcagatggc gctggggaat ttctaatcat
     2401 ggatctgtgg catagcatag acggcttgaa cactttcttt gctgataaac acgtccagga
     2461 gggcgggaat atgatcttca gtcaacgcga cccggtcgtg tgggcgcctg cagaaggttt
     2521 caccagtttt catatcccgg ctccctctgg caataatgat cgtgtcattg cggttgtgcg
     2581 cggcacagtt aattcaaaag aagaagcgaa gaaattacat aatgcggtca tgtccaaaac
     2641 catcagcaag gcgcgctccc tgggactgct ctcccatgaa tcctacttcc gcctggccgc
     2701 gccggattct ccagaagcaa tggaatacct gggcgtggat gtttggatga acggcgaacg
     2761 cctgggtgaa tactacggtg atgaagaatt cctggcaggc tatgacggtt tcttcaccgc
     2821 cgaagccaca gactcagtct gggtgcatcc caaaggtgaa tggactgagt ggtaacagtt
     2881 tagattgtca aagaaaagag cgaccttgcg gtcgctcttt tctttgacaa tctaagtgat
     2941 taccactcag tccattcacc tttggggtgc acccagactg agtctgtggc ttcggcggtg
     3001 aagaaaccgt catagcctgc caggaattcc tcatcaccgt agtattcacc caggcgttcg
     3061 ccgttcatcc aaacatccac gcccaggtat tccattgctt ctggagaatc cggcgcggcc
     3121 aggcggaaat aggattcatg ggagagcagt cccagggagc gcgccttgct gatggttttg
     3181 gacatgaccg cattatgtaa tttcttcgct tcttcttttg aattaactgt gccgcgcaca
     3241 accgcaatga cacgat
//
LOCUS       DASTJP010000138         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1477064, whole genome shotgun sequence.
ACCESSION   DASTJP010000138 DASTJP010000000
VERSION     DASTJP010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477064"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VFQ23_04200"
     CDS             complement(<1..1268)
                     /locus_tag="VFQ23_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905810.1"
                     /translation="MMVQKSDFPVIEGMRCLWSMLNFSRICSILAGFALLILPTPSMK
                     ARVLILPSILWLVSCLPGTAPVANSPALHPTGFNTQAPPNASPTAPHLVTTGEPMPTP
                     STGWFELVGHSPLLERGMNAGLAVHGSYAYVGSRTDGRHANAGVLVVDISNLANPQVI
                     YQIGSPEEALVGQTSRELRVWPEQELLLVMNVDCGAVLHDCGQPVTSPNLNIYDISGA
                     NAALPKLILTFPLGRTPHEMFLWDDPQVDGRALLYLSTFGAAIDKLFILDISQARNGV
                     ISEVGAWSPPFGSSHSGLHSLSLSVDGRRGYLAHDEDGFMILDTSQYADALPNPQASL
                     MTPPENWAKSPVGAHSAVKLFGKPYALITEEVYGLCPWGWTYLIDITDESRPFFVSEY
                     KLFPYNTIEHCAGTTYEQNRYTSFSSHNPTL"
     gene            complement(1249..1548)
                     /locus_tag="VFQ23_04205"
     CDS             complement(1249..1548)
                     /locus_tag="VFQ23_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905811.1"
                     /translation="MSNQARADKERYERQLNHYRSLMKVNKRDLDGKPFLAVQIDFWL
                     ESIRFLITELEIWDYMGKTAKYYETINKIHKALNRVHRLILYLDEGDAKDDGPEI"
     gene            1813..>2585
                     /locus_tag="VFQ23_04210"
     CDS             1813..>2585
                     /locus_tag="VFQ23_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET9905812.1"
                     /translation="MKQNKKLGIYLLFGLIAAMALIAIGLFLAPRLAWRLDNLRVAIR
                     RYFNPPEQVLFTPQQQIDIIVQSTLTAMAAPTSTPIVMQTATPQPSAIPTLTFTPIAQ
                     SALLTGIRHEYQQFNNCAPANLSMVLSYWGWQGDQFATRAYLRPSHEIDDKNVNPFEM
                     VNFVESSTTYDALWRVGGDIALLKRLVAAGFPVLIEKGLHSSHDAWLGHYQTVTGYDD
                     ARGQFLVYDSLEGPPEAYGVPYSVVGQFWRHFNFVYVVV"
BASE COUNT          650 a          679 c          643 g          613 t
ORIGIN      
        1 agtgtcggat tgtgtgatga gaagctagta tatctatttt gctcataggt tgtccccgcg
       61 cagtgctcga ttgtgttgta gggaaaaagc ttgtattcag aaacaaagaa cggtcggctt
      121 tcgtccgtga tgtcaatcag atacgtccat ccccacgggc acaaaccata tacctcttcg
      181 gtgatcaggg catacggctt gccaaatagt ttcacggcac tgtgcgcacc aaccggcgat
      241 ttcgcccaat tctctggcgg tgtcataagc gaggcttgcg ggttgggcag ggcatcagca
      301 tactgcgatg tatccaggat cataaagcca tcttcatcgt gggcaagata gccacgccgt
      361 ccatcgacgg aaaggctgag ggagtgcaat ccgctatgag aagatccaaa gggcggactc
      421 caggctccaa cctccgagat gactccattc cgtgcctgtg agatatctaa tatgaatagc
      481 ttatcaatcg ctgctccaaa agtagagaga tatagaaggg cacgcccatc cacctgcgga
      541 tcatcccaga gaaacatctc atgcggagtg cgccccagtg gaaaggttag gataagtttc
      601 ggcagggctg cgttcgcgcc agagatatcg taaatattga ggttgggtga cgtcacaggt
      661 tgaccacaat catgtagcac ggccccgcaa tcgacgttca tgaccaggag caactcctgt
      721 tccggccaaa cacgcagctc gcgagaagtc tgaccgacca gcgcctcttc aggcgacccg
      781 atctgataaa tcacctgcgg attcgccagg ttggaaatat ctacgacaag gacacccgca
      841 tttgcatgac gaccatccgt ccggctgccc acataagcat aacttccgtg aacagccagc
      901 ccggcgttca taccgcgttc gagaagcggc gaatgtccca ccaactcgaa ccatccggta
      961 gatggggttg gcatcggctc acccgttgtc accaaatgtg gcgccgttgg agaggcgttt
     1021 ggaggagctt gggtattaaa gcctgttggg tgaagcgcag gcgaatttgc cactggtgct
     1081 gtaccaggca aacaagatac cagccacaaa atggagggga gaatcaggac acgcgctttc
     1141 atcgatggag ttggaagaat gagcaaagca aaccccgcca gtatagagca aatacggctg
     1201 aaattcaaca tcgaccagag acatctcatc ccttctatga cagggaaatc agatttctgg
     1261 accatcatcc tttgcatccc cttcatccaa atagagaatc aaccggtgca cgcggtttag
     1321 agctttatgg attttgttga tggtctcata atatttcgca gtctttccca tgtaatccca
     1381 tatttcaagc tcagtgatca gaaagcggat gctttccaac caaaagtcga tttgaaccgc
     1441 cagaaatggt tttccatcca ggtcgcgttt atttaccttc atcaacgacc ggtaatgatt
     1501 taactgacgc tcgtatcgtt ctttgtccgc ccgagcctga ttgctcattg tgagttactt
     1561 ttcaaggaga taaatcgcac atggttgcct ctgtatccca gggatgtagt ctattcatag
     1621 tatatatcga aaaactcaaa gttcgattac tcgagttatc ttttcatcgg caggaacctg
     1681 agtagaacga gagacgtttg ttagtagtaa agggaacgac gcgctgaaac cagcaaagaa
     1741 gattattaaa gagacgatag acttacccgc ctgtcagttc cggaaagtta taatcattgg
     1801 attggatgac aaatgaaaca gaataagaaa cttggaatct acctgctctt cggcttgatc
     1861 gccgcgatgg cgcttatcgc catcgggttg ttccttgcgc cacgtcttgc atggcgactg
     1921 gacaatctgc gcgttgcgat acgacgttat ttcaacccac ccgagcaagt actctttaca
     1981 ccgcagcagc aaattgacat catcgtgcag agcacgctca cagccatggc tgcgccgacc
     2041 tctacgccta tcgttatgca aacggcaacc ccacagccat ctgctattcc caccctcacc
     2101 ttcacgccca tcgcgcaaag cgccttgctt acaggcatcc gacatgagta tcaacagttc
     2161 aacaattgcg cgccggcgaa cctctcgatg gtgctctcct actggggctg gcaaggcgat
     2221 cagtttgcga cccgcgccta tctacgcccc agccacgaga tcgatgacaa gaatgttaac
     2281 ccgtttgaaa tggtgaactt cgtcgagagc tccacaacat acgacgcact ctggcgcgtg
     2341 ggtggtgata tcgccctgct caagcgattg gttgcggctg gcttcccggt gctcattgaa
     2401 aagggtttac attcctcgca cgacgcctgg ctcggtcatt atcagacagt cacgggatac
     2461 gatgatgcgc gcggtcaatt cctggtctat gactcgctcg aaggtccacc agaagcctat
     2521 ggtgtaccct attcggtggt cggtcagttt tggcggcact tcaattttgt ctatgtggtg
     2581 gtctt
//
LOCUS       DASTJP010000139         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_852111, whole genome shotgun sequence.
ACCESSION   DASTJP010000139 DASTJP010000000
VERSION     DASTJP010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852111"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            532..981
                     /locus_tag="VFQ23_04215"
     CDS             532..981
                     /locus_tag="VFQ23_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905813.1"
                     /translation="MSPAKSTSRTAPQKSGKFTAEEKAAMKERAKELKAEAQKADGES
                     AALAAIDAMPEPDHSMAKRIHAIIKSSSDLAPKTWYGMPAYANEEGKVICFFTPASKF
                     KTRYATFGFNEDAKLDDGVMWPTSFALKKLTATEEAKIAALVKKAMS"
     gene            1035..1646
                     /locus_tag="VFQ23_04220"
     CDS             1035..1646
                     /locus_tag="VFQ23_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905814.1"
                     /translation="MSRNQKRMETTHERIKWSPRLRPALLKRLYESDALGLQDDDLCN
                     DVGARLYLRCQTVLMVSRNEVACPRCGASFKVDTSSQGAITLCPTKDCGWQVSLTDYH
                     QSWSKKRIWGANALPAFEEFYERFSIKLPYKEKMLLIDQLIHSFHWSMQQMLPARSAA
                     NNLIEGEHDGVIEFLDGLTGIDPARKAEWRETMHRMMKRRKGK"
     gene            complement(1970..>2168)
                     /locus_tag="VFQ23_04225"
     CDS             complement(1970..>2168)
                     /locus_tag="VFQ23_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905815.1"
                     /translation="SQSQTFGPDSNNLVDTLAIKTLSLQADTICSSGDKWDSTLITLS
                     GVGRNFFSGETRGRISITFVP"
BASE COUNT          594 a          539 c          569 g          466 t
ORIGIN      
        1 ttcaataata ttactgcaac atggtggctg ttcgatgcgg caacgaatcc atcatttgat
       61 catccagcta tgagatctat taatttgcca ttgggacaga tccgtgttga aacagtctat
      121 tgtgaaaaca aaaagaatgc agcgattgtt catagatttt gggaagctta gtaataagac
      181 aacgctgctt atgcctccca gtgggcggtt tctttataga taggttgtag ttattttagg
      241 aagtagaaaa gaaataacag aacagtattt gatggtcgtg cctggcctga aaatggttgc
      301 ggttgccaat cacgaatggc agatttcgca tgaggaagcg aatctacaga agtatgcgtt
      361 cacagatcgt atattcgacc gttggtaagc gcatggcagg cggaataata aagtatgaga
      421 atctgattag accctccatc ttttgtcatt ttgccacttt ctcgttcgac gttaaaatag
      481 aacggcaaaa aaatacgccg taatccatca agtacaggta aggagtttca aatgagccca
      541 gccaaaagca ccagccgaac agccccgcaa aaatccggca aattcacagc cgaagaaaaa
      601 gcagcgatga aggaacgcgc caaagagcta aaggcagaag cgcaaaaggc agacggagaa
      661 agcgccgcgc tggcagccat cgacgccatg ccggaacccg atcacagcat ggcgaagcgc
      721 atccatgcca tcatcaagtc cagctcggac ctcgcgccga aaacctggta cgggatgccc
      781 gcctatgcca acgaagaagg gaaggtcatc tgcttcttca cacccgcatc caagtttaag
      841 acccgatacg ccaccttcgg ctttaacgaa gatgcgaagc tcgatgatgg cgtcatgtgg
      901 cccacgtcct tcgcgttgaa gaagctcact gccaccgaag aggcaaagat cgccgcgctc
      961 gtcaagaaag ccatgagctg agggttgctc ccgccgtagg atgtggagca aaaaccaatc
     1021 agtagacacg ggacatgagc cggaatcaga aacgaatgga aacgacgcac gagcgcatca
     1081 aatggtcacc gcgcctgcga ccagccctgc tcaaacggct gtatgaatca gacgcgctgg
     1141 ggcttcagga cgatgatctc tgcaatgacg tgggagcgcg gctctatctg cgctgccaga
     1201 ccgtcctcat ggtgtcgaga aatgaagtgg cttgcccgcg ctgtggagcc tcgttcaaag
     1261 tggatacctc cagccagggc gcgatcaccc tttgccccac aaaagattgt ggctggcagg
     1321 tctcgctgac tgactatcac caaagctgga gcaagaagcg catctgggga gcaaatgccc
     1381 tgcccgcctt cgaagaattc tacgagcgct tttccatcaa attgccctac aaggaaaaga
     1441 tgctgctcat cgaccagctc atccatagct tccactggag catgcagcaa atgctcccag
     1501 cccgctcggc tgccaacaac ctgatcgaag gcgagcatga cggggtcatc gagtttctgg
     1561 atggactgac cggcattgac ccggctcgca aagccgaatg gcgcgagacg atgcacagga
     1621 tgatgaaaag aagaaaaggg aaatgaaaaa attccctctc ccgacgggag aggggtaggg
     1681 gtgagggtga attacacctc catctccgca aaatgctcca tcaatacatg aaagatgata
     1741 taactcctgc cgatgcagtt gagcagtcta tcgtgtggat tctcttaaac taagctgcct
     1801 gttataaatt tgcgcacttt gcttgcttag tcgatgactg catcgcccac atagccgtga
     1861 ctctgtgcgg cggtgaccag tccatcgatg ctgacactgt accacttgag attgggtttt
     1921 ggctttgtgg ctacttacga gagcccgagt cacacgatat attttgaagc tatgggacaa
     1981 aggtgatcga aattcttccg cgagtctctc cggagaagaa gttgcgacct acaccgctca
     2041 aagtgatcag ggttgaatcc catttgtccc cgctagaaca gatcgtgtcg gcttgcagac
     2101 tgagggtctt gatggcgagt gtgtcaacca ggttgttgga atccggaccg aaggtttggg
     2161 attggctg
//
LOCUS       DASTJP010000140         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_850901, whole genome shotgun sequence.
ACCESSION   DASTJP010000140 DASTJP010000000
VERSION     DASTJP010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850901"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFQ23_04230"
     CDS             <1..272
                     /locus_tag="VFQ23_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET9905816.1"
                     /translation="WEIAKEKIASRNYDLIILDEFTYTLHYGWLNTNEVLEWLKENKA
                     EDMHLIITGRDAPPALIEYADLVTEMLEVKHPYQKGIQAQPGIEF"
     gene            275..1333
                     /gene="cobT"
                     /locus_tag="VFQ23_04235"
     CDS             275..1333
                     /gene="cobT"
                     /locus_tag="VFQ23_04235"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET9905817.1"
                     /translation="MQLDEIIKQIQPLDETAMRTARLRQDALTKPRGSLGRLEELSIQ
                     LAGITASPLPSVKQKTVIVMAADHGVTSEGVSAYPAEVTPQMVLNFLHGGAAINVLAR
                     QANARVVVVDMGVASEIDDTSGALIRCKVGLGTANMAKGPAMTREQARESIQSGIKIA
                     ESEIARGVDLLATGDMGIGNTTSAAAIACAFMDQLPQHIAGYGTGIDEVGLKRKIDVI
                     ALALQLNNPNQNDALDVLAKVGGFEFGGLAGVMLASAANRKPVVIDGFISTAAAMIAA
                     TLAPAVRDYMIAAHVSQERGHKLMLEWLGLKPLLDLNMRLGEGTGAALAFHLIEASTR
                     ILHEMATFGAAGVSEKDS"
     gene            1434..2174
                     /gene="cobS"
                     /locus_tag="VFQ23_04240"
     CDS             1434..2174
                     /gene="cobS"
                     /locus_tag="VFQ23_04240"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HET9905818.1"
                     /translation="MVSIIAAFQFLTISPTLIKRMFTSQEMGRAVGWFPLVGVVLGFL
                     LYGVIYAARLIFPVSVSAAISLFAWVIFTRAFHLDGFMDTCDGLFGGWTPERRLEIMK
                     DSRMGAFGVAGGILVLITEYSALTSATNLFPAIILATTLGRWASPLVIYAFPYAREDG
                     LGIEMKRNVNIKEVILATLLAGVTAWFVAGWMGFGLMLGAAGIGFLVALYAMRLLSGL
                     TGDIYGTVTTVVEMLILVVFTIKDFGSA"
BASE COUNT          526 a          500 c          621 g          575 t
ORIGIN      
        1 gctgggagat tgcaaaagaa aaaatcgcca gccggaatta tgatctgatc atcctggatg
       61 aatttacata tacactgcat tacggctggc tgaataccaa tgaggtcctt gaatggttga
      121 aagaaaataa ggccgaagat atgcatctca tcatcaccgg acgggatgca cctcctgcct
      181 taatcgagta tgccgactta gtgacagaga tgctggaagt aaaacatcct tatcaaaaag
      241 ggattcaggc acaaccgggg atcgagttct aaggatgcaa ctcgatgaga tcatcaaaca
      301 aatccaacct ttggatgaaa ctgcgatgcg gacggcgcgt ctgcgccagg atgcattgac
      361 caagccacgt ggcagtctgg ggcggttaga agaactttcc attcaactcg ccggaatcac
      421 tgcttctccg ctgccttctg tgaaacagaa aactgtcatc gtaatggcag cggaccacgg
      481 tgtgaccagc gaaggagtga gtgcttatcc tgccgaagtg actccgcaga tggttctgaa
      541 ttttttacat ggtggcgcgg cgatcaatgt gttggcgcgt caggcaaatg cacgagtggt
      601 ggtagtggat atgggtgtcg cctcagagat cgatgacaca agcggggcac tcatccgctg
      661 caaggtaggt ctgggcaccg cgaacatggc gaaaggtccg gcgatgacgc gcgaacaggc
      721 aagagagtcg atccaaagcg ggatcaaaat cgctgagtca gaaatcgcgc gtggggtcga
      781 tctcctggca accggtgata tgggtattgg taataccacg tctgccgcgg caattgcatg
      841 tgcattcatg gatcagcttc cccagcacat cgcaggatat ggcaccggta tagatgaagt
      901 tggtttaaag agaaagatcg atgtaattgc tctcgcttta cagctgaata acccgaatca
      961 aaacgatgca ctcgatgtgc ttgcaaaagt cggtggtttt gaattcggtg ggttggcagg
     1021 tgtgatgctg gcatctgctg caaaccgcaa gcctgtggtg atcgatggat ttatctccac
     1081 ggccgcggcg atgattgccg cgactcttgc gccggctgtg cgggattata tgatcgctgc
     1141 acatgtctca caggagcgcg gtcacaaatt aatgctcgag tggctgggct tgaaaccgct
     1201 gctcgatttg aatatgcgcc tcggtgaagg cacaggcgcg gcgctggcat tccatttgat
     1261 tgaagcttcg acgcgcatcc tgcacgaaat ggcgacattt ggtgcagcag gtgtttctga
     1321 aaaagattct taaccacgaa ggactcaaag gtttgtaata ttgcacaaca taagcccgcc
     1381 ctttggattt ttctttgtgc tcgtagtgtc cttcgtattt aaagaaaaat aacatggtct
     1441 cgattatcgc cgcgtttcaa tttctgacca tttcgccaac cctcatcaag cggatgttta
     1501 catcgcagga gatgggtcgg gcggtgggat ggtttccgct tgtgggcgtt gtgctgggct
     1561 ttttgttata tggcgtgatt tacgccgcgc gtttgatttt cccagtatca gtttcggcgg
     1621 caattagctt atttgcatgg gtcattttca cccgtgcctt tcatctggat ggattcatgg
     1681 atacgtgcga tggcttgttc ggtggctgga ctccggaacg caggctggag atcatgaagg
     1741 actcgcgtat gggtgccttc ggtgttgcag gcggaattct ggttttgatc accgagtatt
     1801 ctgcgctcac ttcagcgaca aacctctttc cggcaatcat actcgctact acgctgggac
     1861 gttgggcctc gccgcttgtg atttacgcct tcccctacgc ccgcgaagat gggcttggca
     1921 ttgagatgaa gcgtaacgtc aatatcaagg aagttatcct tgccacgttg cttgcagggg
     1981 tcacagcctg gtttgttgcg ggctggatgg gatttggatt aatgcttggc gcggcaggga
     2041 ttggttttct tgttgccttg tatgctatga ggttactctc tggacttaca ggtgatattt
     2101 atggaacagt cacaacagtt gttgagatgt tgatattggt tgtgttcaca atcaaagact
     2161 tcggaagcgc ctaaaaacgg cgagacttcc gaaatctgaa agaactcatg tcaaaaatta
     2221 cc
//
LOCUS       DASTJP010000141         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1411801, whole genome shotgun sequence.
ACCESSION   DASTJP010000141 DASTJP010000000
VERSION     DASTJP010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411801"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VFQ23_04245"
     CDS             complement(<1..967)
                     /locus_tag="VFQ23_04245"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET9905819.1"
                     /translation="MNKATEAVILSAARTPIGKFQGALSSVPATQLGAIAVKAAVERA
                     GINPEEIEEVILGNVVQAGEGQAPARQSGIYAGVPATVSASTINKVCGSGLKAAMLAS
                     QAVRAGDADLFVAGGFESMSRAPYLVSGRSGELKFGNQALTDALLSDGLWDPFENWGM
                     GNAAEFIGEEYEVTREAMDRWSLESHQKAVAAQEAGKFKEEIVPVQIPGRKGEVTVID
                     KDEGPRKDTSLEALSKLKPAFKPDGKVTPGNSSPMNDGGAAVVISSRDYAEKNNLKPL
                     ARIVGYTQAAVEPKYLFAAPAQAIPKLLNKIGWTLNDVDLIELNEA"
     gene            1033..1425
                     /locus_tag="VFQ23_04250"
     CDS             1033..1425
                     /locus_tag="VFQ23_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HET9905820.1"
                     /translation="MINNQPSLYELIGGEQELRKLVDRFYDLIDSSPEATQLRSLHPK
                     SLKQSREKFFMFLSGWSGGPQLYIEKYGHPRLRMRHMPFSIGIEERDQWIWCMNKALD
                     EMQMNPAVIEYLKARFAESAEFLRNKKE"
     gene            complement(1499..2794)
                     /locus_tag="VFQ23_04255"
     CDS             complement(1499..2794)
                     /locus_tag="VFQ23_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HET9905821.1"
                     /translation="MTDRVLQKFDKKFVLRVLSGLFVAILISVVSITPANSQGVKIHF
                     GETKIENHFPDGLRFSVKIDVADQRGTIDFYYKLGNEPWAHQDARCSANGTVIDLDYY
                     SCIFFLDIQNIPPQLPITYKWRIYGSAQRYSDEQTVHYENPCYVWETFTRGNITIWWH
                     DRPQEFAERVFLIAEKSIQEQQSLFEVNLSNPIHLVVENSREENSMWNSRIPYSIGGQ
                     AFPEVGVTIQIVELDMSEFLVDSWLNEVVPHEISHLYFFQATNREHASPPNWLDEGLA
                     GYNEFASHQFDWILVKNAIRQDKLIPLADLRDAFSGDDDQVALSYSESMTAAEYLIET
                     YGEQGLNDLFSAYQEGKTSEEAFQQAFGRTVSEVETDWEAWARAKPDEFSIGALIGIF
                     FVGAYFAGGCASAFLLIGLILFLSRKKPMQKTVNGTAPG"
     gene            complement(2851..>3420)
                     /locus_tag="VFQ23_04260"
     CDS             complement(2851..>3420)
                     /locus_tag="VFQ23_04260"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET9905822.1"
                     /translation="YLGIKTPQSKALQKEFYAEYGAPEVTELDSILRDLWSLPQREFQ
                     YFGLGLLDKLEKKLPAKFIETIEYLIITKSWWDTVDTLASHAVGSHFRRFPNVLEKYL
                     PKWRSSKNMWLRRTGILFQLNYKNETDFELLCEIIRENLDSKEFFINKAIGWALRQYA
                     RVDPKAVKKFVNSTPLHPLSTREAMKHLR"
BASE COUNT          871 a          864 c          760 g          925 t
ORIGIN      
        1 aggcttcgtt gagttcgatc aggtcaacat cgtttagtgt ccacccgatc ttgttcaaca
       61 gttttggaat cgcttgtgca ggggcggcaa aaagatattt tggctccaca gctgcctgcg
      121 tgtaaccaac aatcctggca agtggtttga ggttattttt ttctgcatag tcacgtgatg
      181 aaatgacaac ggccgcgcca ccatcattca tgggagatga attgccgggg gtcacttttc
      241 catctggctt gaaggcaggc ttgagtttcg acaaagcctc cagcgatgta tccttacgtg
      301 gaccttcatc cttgtcgatg actgtcactt cgcctttgcg ccccgggatt tgaacgggaa
      361 cgatctcctc tttgaatttt cctgcctcct gtgcggccac tgccttctga tgactttcaa
      421 gcgaccagcg atccatcgcc tcgcgcgtga cttcatactc ttcaccgata aattcggctg
      481 catttcccat gccccagttt tcaaacggat cccagaggcc atcgctgagc agggcatcgg
      541 tgagggcctg gttgccgaac ttcaactcac ccgaacggcc ggataccaga tacggcgcac
      601 ggctcatgga ctcaaatcca ccggcgacaa atagatctgc atctcctgca cgaactgcct
      661 gcgaggccaa catcgcggct tttagccccg agccacagac tttgttgatc gtgctcgcgc
      721 tgactgtggc aggtacgcca gcatagatcc ctgattgacg tgctggagct tgtccctcgc
      781 cagcttgcac cacgttccct aaaatgacct cttcgatttc ttccgggtta atgcccgcgc
      841 gctccaccgc agctttgacg gcgattgctc ccaattgcgt ggcaggcaca ctgctcaacg
      901 cgccctggaa tttaccaatc ggtgtgcggg cggcagaaag gataacggct tcggttgctt
      961 tgttcattac agctcctgag aaaatataaa cagggtaatt ataaagccta tgtcatcagt
     1021 tacaatttca ggatgatcaa caatcaacct tccctatatg aattgatagg cggcgagcag
     1081 gagttgagga aactagtcga ccgattctat gatctgatcg attcctcccc tgaggcaaca
     1141 caacttcgat ccctgcaccc caagagtcta aagcaatcgc gtgagaaatt cttcatgttt
     1201 ctttccggct ggtctggcgg accgcaactg tacatcgaaa aatatgggca tccccgacta
     1261 cgaatgcgcc acatgccgtt ttcgattgga atcgaggaac gagatcaatg gatctggtgc
     1321 atgaacaagg ctttggatga aatgcaaatg aacccggcgg tgatcgaata cctcaaggct
     1381 cgctttgctg agagcgccga gtttctacga aataaaaagg aatagtctga tttctaaacg
     1441 cgtaagcttc ttggatgaat gggctgttat ttaatcaaga agcttcgttg tcgcttttct
     1501 aacctggtgc agtcccgttt acagttttct gcattggttt ttttctactt aaaaatagga
     1561 tcaacccgat tagcaaaaat gccgaggcac agcctccggc aaaataggct cccacgaaga
     1621 agatcccaat gagagctccg atggaaaatt cgtcgggttt ggctcttgcc caggcttccc
     1681 aatccgtttc cacctcactt acggtacgtc caaatgcctg ttggaatgcc tcctcggagg
     1741 tcttaccttc ctgatacgcc gaaaacaaat cgttcagacc ttgctcacca taggtttcaa
     1801 taaggtattc ggcagcagtc atactctctg agtatgacaa agccacctgg tcatcatctc
     1861 cgctgaacgc gtctcgtaaa tcggcaaggg gaattaattt atcctgtcgt atcgcgtttt
     1921 ttaccaatat ccagtcaaat tggtgactgg caaactcgtt ataaccagct aatccttcgt
     1981 ccagccagtt gggcggactc gcgtgctcgc gatttgtagc ttgaaagaag tacagatgtg
     2041 agatttcatg agggacaacc tcattcaacc atgaatccac tagaaattct gacatatcca
     2101 gctctacgat ttgtatggtc acaccgacct ctggaaatgc ctgaccgcca attgaataag
     2161 gaattcggga attccacatc gaattctctt cccgcgaatt ctccactacg agatgaatgg
     2221 gattggataa gttgacttca aataaagact gttgttcctg aatggatttt tcagcaatta
     2281 gaaagacacg ttctgcaaat tcctgtggcc gatcatgcca ccagatcgtg atattccccc
     2341 tggtaaacgt ctcccataca taacaaggat tctcataatg aaccgtttgc tcatcactat
     2401 atctctgtgc actaccataa atacgccatt tataggtaat cggtaattgt ggaggtatat
     2461 tttgaatatc cagaaagaaa atacaactgt aataatcgag atcgatcacg gtgccattgg
     2521 ccgagcatct ggcatcctgg tgcgcccagg gttcattacc cagtttgtaa taaaagtcaa
     2581 tggtgcctct ttgatctgca acgtcaatct tgacgctgaa tcgtaggccg tcgggaaagt
     2641 gattctcaat cttggtttcc ccaaagtgta ttttcacacc ctggctattc gccggcgtaa
     2701 ttgaaacaac cgaaatgagg atggctacga ataaccctga taacactcga agcacgaatt
     2761 ttttgtcgaa tttttgaagt acccggtccg tcatgtattt acaattccac tccatctctt
     2821 ggcaaccagc atttatatca agacccaatt tcaccgcaaa tgtttcatcg cctcgcgcgt
     2881 actcaacgga tggaggggcg tggaattgac aaacttcttc acagcctttg gatctacccg
     2941 cgcatattgc cgcaaagccc agcctatggc tttgttgata aagaattcct tcgaatccaa
     3001 attctcgcgg atgatctcac agagcaactc aaaatccgtt tcatttttgt agttgagctg
     3061 aaacagaatc cctgtgcgtc gaagccacat atttttggaa gagcgccatt ttggaagata
     3121 cttttctagt acgttcggaa agcggcggaa gtgactccct actgcatggc tggcaagtgt
     3181 gtccactgta tcccaccagg attttgtaat aattaagtat tcgatcgttt cgataaattt
     3241 tgctggaagc tttttctcta acttatccaa caaacccaga ccaaaatact gaaattcacg
     3301 ctgcggcaaa gaccaaagat cgcgtaggat ggagtcgagt tctgtgactt caggtgctcc
     3361 atactcagca tagaattctt tttgcagagc cttgctttgt ggagttttaa taccaagata
//
LOCUS       DASTJP010000142         4170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1392455, whole genome shotgun sequence.
ACCESSION   DASTJP010000142 DASTJP010000000
VERSION     DASTJP010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392455"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFQ23_04265"
     CDS             complement(<1..341)
                     /locus_tag="VFQ23_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9905823.1"
                     /translation="MTTAISVHNLSKRYKIGAAQGKFRYGMLRDVFVDTLTAPVRFAR
                     SLAGTSKNGSSAKNDNFIWALNDVSFDLDEGKVLGIVGRNGAGKSTLLKILSRVTEPT
                     KGTVSVRGRVGS"
     gene            complement(344..1189)
                     /locus_tag="VFQ23_04270"
     CDS             complement(344..1189)
                     /locus_tag="VFQ23_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905824.1"
                     /translation="MTELTKHEPTTIYIKPSTGFTALNLRDLWLYRELVFFMVWRDIK
                     VRYKQTILGAAWAILQPVLTMLALYFLFDRVANLPTDNVPYPIFSYTALLPWGLFVTA
                     LNQGSRSLTSNHNMITKVYFPKLILPLSSVLAGLVDFAIASVILVVLMFYYQVSPAWN
                     LIWVLPLFLLLAIITALGVALWLSAINVKYRDVNYVLPFLTQFWFFATPVAYSAKAIS
                     ENWQLVYSLNPMAGVVNGFRWALLGIGNGPDAGLWVSVGISILILISGLFYFRNMEKT
                     FADTI"
     gene            complement(1236..2189)
                     /locus_tag="VFQ23_04275"
     CDS             complement(1236..2189)
                     /locus_tag="VFQ23_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HET9905825.1"
                     /translation="MAENFWNDKRVIVTGGSGFLGSFVVEKLQQRGAGDVFVPRSSTY
                     DLRQPDGIAAMLNDYPADMVIHLAALAGGIGANRARPAEFFYDNLMMGVPLLHQAWQS
                     GVSKFVAVGSVCSYPKFTPVPFKEDDLWQGYPEETNAPYGLAKKMLLVQSQSYREQYG
                     YNSIYLMPVNLFGPRDNFDLETSHVIPALIRKAVEAGERGEAELPIWGDGSPTREFLY
                     VEDAADGIVTAAEKYNENQPVNLGSGYEISIKDLSEMIVKMTGFEGKLIWQTDKPNGQ
                     PRRGLDVSRAKEYFGWSAQVPFEEGIRRTIEWFKENREKIK"
     gene            complement(2202..3212)
                     /locus_tag="VFQ23_04280"
     CDS             complement(2202..3212)
                     /locus_tag="VFQ23_04280"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9905826.1"
                     /translation="MSDRHVLITGGAGYIGSFLTSELLRANYRVTVVDSLLFGGDSLI
                     PFLHHPNFHFVKADVTDPRAIKDSVKDSWQKPDAIVHLAAIVGFPACQAVGKQVAWRY
                     NVEATEKVFEQAADLAVERIVFASTYSNYGLSPEGKPVTEESPLNPQSIYAETKIAAE
                     EFLLSQKESACAPLLFRFATLYGMSPRTRFDLIVNQFVLEAFTKRSLIIYQRGYSRSF
                     VHIQDVVRGVIMGLEAEQSKIRGEVFNLGTDHGNYSKDDIVRLVLKRMPETVVEYKDL
                     TFGGDMRDITVSFAKIRKVLGFETTLDVDDGIRGLLFALKSGIIRNPTDEKYRNAQFI
                     VQ"
     gene            complement(3209..3682)
                     /locus_tag="VFQ23_04285"
     CDS             complement(3209..3682)
                     /locus_tag="VFQ23_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET9905827.1"
                     /translation="MESTNEERELTLLEKIEKDPDVNQSALATQLGVAVGTVNWHLKR
                     LISKGYVKVKRAERKKLRYIITPEGIALRARLAVDYVEKSFSLYRKTRQRVREFISEV
                     QTAGYDRLRIIGKGDVADICQLTCLEHGIKVVTDKSVPALQVDGSKVILQMEKSS"
     gene            3818..>4170
                     /locus_tag="VFQ23_04290"
     CDS             3818..>4170
                     /locus_tag="VFQ23_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905828.1"
                     /translation="MKLKQLTGWFSNPSRREILPALAALFIALAINVVVLIIPPPALI
                     TRSLFNFRFDVAVLIITFLALIFSRKGIAWDTISLTLTLVLFSVPLIYKWQSAGFYGY
                     LIGGLLPWSDAAGYYS"
BASE COUNT         1072 a         1073 c          969 g         1056 t
ORIGIN      
        1 gatccaacgc gtccgcgaac ggagaccgtc cctttggtgg gttcggttac gcgggaaaga
       61 attttcagaa gagtgctctt gccggcgccg ttgcgtccga caatgcccag cacttttcct
      121 tcgtcgaggt caaacgaaac gtcgttcagt gcccagataa aattgtcgtt tttcgcagat
      181 gaaccatttt tggaagtgcc cgcaagtgat ctggcaaatc gaacaggtgc agtcagcgta
      241 tcgacgaaca catcgcgcag catcccataa cggaacttgc cttgtgccgc gccgattttg
      301 tatcgtttgc ttaaattatg gacactgatt gctgtggtca tgattagatt gtgtctgcga
      361 aggtcttttc catgttgcgg aaatagaaca agccactgat caggatcagt attgaaatgc
      421 caaccgaaac ccaaagcccg gcatcaggac cgttgccaat tcccagcaat gcccagcgaa
      481 atccgttcac cacacctgcc atcgggttca atgaatagac gagctgccag ttctctgaaa
      541 tcgctttggc agaataagcc accggagttg caaaaaacca gaattgagtc aggaaaggaa
      601 gcacatagtt aacatcgcga tatttgacgt tgatcgccga gagccacaag gccacgccca
      661 aggcagtgat gattgccaaa aggagaaaga gtggcaatac ccagattaaa ttccaggcgg
      721 gcgaaacctg gtaatagaac atcaagacaa ccaatatcac ggaggcaatg gcaaaatcca
      781 ccagccccgc cagcacggac gataacggca ggatcaactt tggaaaatat accttggtga
      841 tcatgttgtg attggaggtc aacgaacggc ttccctgatt gagcgcggtg acaaacaacc
      901 cccagggaag cagcgcggta tatgaaaaaa tcggataagg gacattatcg gttggtaaat
      961 ttgccacacg atcaaacaaa aaataaagcg ccagcatggt caacaccggc tgcagaattg
     1021 cccaggctgc cccaagaatc gtttgcttgt aacggacttt gatatcgcgc cagaccatga
     1081 agaagaccag ttcgcgatac agccagagat cacgcagatt cagcgcggta aaacccgtgg
     1141 aaggcttgat gtaaatggtt gtcggttcgt gcttggtgag ttcggtcatt atttatttca
     1201 gtcattgcga gggcgttagc ctcgcaatga caccattact ttatcttttc tcgattctct
     1261 ttaaaccact caatcgtgcg tcgaatccct tcttcaaatg ggacttgtgc gctccagccg
     1321 aaatattctt tcgcgcgtga gacgtcgagt ccgcggcgag gctgaccatt gggcttatcc
     1381 gtctgccaga tcagtttccc ttcaaagccg gtcatcttca cgatcatttc acttaaatct
     1441 ttaatggaaa tttcataacc agagccgagg ttgactggtt gattctcatt gtatttctct
     1501 gcagccgtca caatgccatc agctgcgtct tccacgtaga ggaattcacg tgtgggggag
     1561 ccgtcacccc aaattggcaa ttccgcctcg cctcgttcgc cagcctcaac tgcttttcgg
     1621 atcaaggccg ggatgacatg tgacgtttcc aggtcaaagt tatcgcgcgg accgaagaga
     1681 ttgactggca tgaggtaaat cgagttatat ccatattgct cacgataaga ctgggattga
     1741 acaagtaaca tcttctttgc caatccataa ggcgcatttg tctcttccgg atagccctgc
     1801 caaagatcat cctccttaaa gggcacggga gtgaattttg gataagagca aaccgagcca
     1861 actgccacaa atttgctcac accgctttgc caggcctggt gcagcaacgg cacacccatc
     1921 atcaagttat cgtagaagaa ttccgcgggt ctggcgcggt ttgcgccaat gcctccagcc
     1981 aaagcggcca aatggataac catatccgcc ggataatcgt tcagcatggc ggcaattcca
     2041 tcaggctgac gcagatcata cgttgaactg cgcgggacaa aaacatcccc agcaccgcgt
     2101 tgttgtaatt tttcaacgac gaaggagccc agaaaacctg agccccctgt gacaattacg
     2161 cgtttgtcgt tccagaaatt ttcagccatg gttctcctga actattgcac aatgaattgc
     2221 gcgtttctat atttctcgtc ggttgggttc cggataatac cggacttcag tgcaaacaac
     2281 aatccccgaa taccatcatc cacatccaac gttgtctcaa acccaagcac cttcctgatt
     2341 tttgcaaatg acacggtgat gtcgcgcata tcccccccga aggtcaaatc cttatactca
     2401 acgacagttt ctggcatacg cttgagtacc agcctcacaa tatcatcttt tgaataattt
     2461 ccgtgatctg ttcccagatt gaatacttcg ccgcgaattt ttgattgttc agcttcaagc
     2521 cccataatca ccccgcgtac gacatcctgt atgtggacaa acgaacgcga atacccgcgc
     2581 tgatagatga ttagagaacg tttggtgaag gcttccagca cgaactgatt cacgatcagg
     2641 tcaaagcgtg tacgtggaga catcccataa agtgttgcga accgaaagag caagggtgca
     2701 caagctgact ccttttgtga aaggagaaat tcctctgccg cgatctttgt ctcggcatat
     2761 atcgattgtg gattcaacgg agattcttca gtaacaggct tgccttccgg ggagagtcca
     2821 tagttgctat aggtggatgc gaacacgatt cgttctacgg ccaggtctgc ggcctgctcg
     2881 aacacttttt ctgtggcttc gacgttatat cgccaggcaa cctgcttccc aacggcctga
     2941 caagctggga acccgacaat tgccgcaaga tggacaatcg catctggctt ctgccagctg
     3001 tcctttaccg aatctttgat ggcgcgcgga tcggttacat ccgctttgac aaaatggaag
     3061 ttgggatgat gcaaaaaggg aatcagcgag tcgccaccga agagaagaga gtcgacgact
     3121 gtaacacgat agttggcgcg gagcagttcc gaggtcagga acgaaccgat atatcctgcg
     3181 ccccctgtaa tcaacacatg gcggtcactc atgaagattt ctccatttgc aagatcactt
     3241 tcgatccatc cacttgaagc gcaggcacgc tcttgtcggt gacaactttg atcccatgct
     3301 caaggcaggt caattggcaa atatccgcaa cgtcgccttt accaataatg cgcagacggt
     3361 catagcccgc ggtttggact tccgagataa attctcgcac acgctgacgc gtcttgcgat
     3421 ataaagaaaa ggatttttcg acgtaatcga ccgccaaacg agcacgtaag gcgatgccct
     3481 cgggtgtgat gatatagcgc agttttttgc gctctgcgcg tttgaccttc acataacctt
     3541 tggaaatcag tcgcttaaga tgccagttca ctgttccaac agccacaccc agttgagtag
     3601 ccagcgcgga ctggtttaca tccgggtctt tttcgatctt ctcaagcagg gtgagctcgc
     3661 gttcttcgtt tgtagattcc attcacaatt ccaaaattca gtgactgaat tataaccccg
     3721 ttcaactctc ggtcaagacc atttcccgtt acgccttcgc aagttattcg gtttgtcata
     3781 aaatcagttt gactataaaa gtcaactttt ccataatatg aaacttaaac aactgactgg
     3841 ttggttttcg aacccctcca ggagggagat acttcctgct ctggcagcac ttttcatcgc
     3901 tctggcaatt aatgttgtcg tacttattat tccccctcct gctcttatca cccgttctct
     3961 attcaatttt cgtttcgatg tggcagttct gatcatcact tttcttgcat tgatcttcag
     4021 ccgaaaagga attgcctggg acacaatcag cctgaccctg actctggtct tattcagtgt
     4081 cccgctcatc tataaatggc agagcgccgg gttttatgga tacctgatcg gaggtttgtt
     4141 accctggtcg gatgcagcag gttattattc
//
LOCUS       DASTJP010000143         5164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_556324, whole genome shotgun sequence.
ACCESSION   DASTJP010000143 DASTJP010000000
VERSION     DASTJP010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556324"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFQ23_04295"
     CDS             complement(<1..910)
                     /locus_tag="VFQ23_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9905829.1"
                     /translation="MAGMERFTQRARSVLSFAHQETERARNNFIGTEHLLIGLMLEGG
                     VAGRVLRELGLSADRLRQTIEKITTVSDNFDPARVELGSQTQKALELAVDEARRLGHH
                     YIGTEHILLGLVLVDDVATEVLRRLGVTAEQIRRQTRRVLNERASPRRIQTSETSAYK
                     DLNQVLLIHGRNMDAADAVASYIVRLGLEVVRSNRPKDRQFDIFATEVIFAVVLLTAD
                     DLVVTKSESDVTKFHASPHVIYELGFFRGKLGYKHVCALFESNPDLSIELPTESFRVV
                     YIPLDSIGNWKVSLAKQMQDAGIRINS"
     gene            complement(975..2543)
                     /gene="dnaX"
                     /locus_tag="VFQ23_04300"
     CDS             complement(975..2543)
                     /gene="dnaX"
                     /locus_tag="VFQ23_04300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET9905830.1"
                     /translation="MTQALYRKYRPKEWEAVVGQDHVVTTLKNAIVADRVAHAYLFAG
                     SRGTGKTTLARLLAKAVNCTNPDPAKRPDNECENCKAVNENRFLDLIEIDAASNTSVD
                     DVRDLRDKINFSPSQGKYKIYIIDEVHMLSTAAFNALLKTLEEPPPHAIFVLATTEIH
                     KIPATVLSRCQRHEFRRVPVGEIVTNLKKIVAAEKIQADDDALIQIARQSAGGMRDAQ
                     SLLDQLSSTGDKITLALAQQVLGTATSQSVLDVLNAVNEHDPAHGLESIHKALDAGAD
                     PRSLARQIVEYLRGLMLIQMGNTNQVEATADVKKQMQAHARSFTTSDVLRMMKAFNNA
                     ATDLRGGWQPSLSLELALAEVLDAPAESAPLAASPRQARPQPTPVHAAESTQSSAQGS
                     SVSGEATDATSKNQSQKPEISAGEVIKVWKHLSGSLPKSQANLSALLNSVRMIDMEGK
                     TLILGLASDVLVSKIDKPDQIEAIQKLIKSHFNVDVNVRCVVTTAKGKIPPNVPQDGM
                     VATAIQHGGEIVDM"
     gene            complement(2683..3177)
                     /locus_tag="VFQ23_04305"
     CDS             complement(2683..3177)
                     /locus_tag="VFQ23_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9905831.1"
                     /translation="MDRRSLFFYLLLNVFISACVTGAILFWYHRNYRSVSLSPVLPSV
                     PSSNPAAVSAATLDPQTDIPVEIVSVIGAGTLNAEWVVVSYTGDDQINLANWQLRDDD
                     GNVFVFPQLVLHTNGAVQVHTDSGTNTVIDLYWSASEPVWESGEEAQLFDPSGNLREV
                     YKVP"
     gene            complement(3272..4975)
                     /locus_tag="VFQ23_04310"
     CDS             complement(3272..4975)
                     /locus_tag="VFQ23_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9905832.1"
                     /translation="MSDSQERDMDNLQPGQMLGSYRIINQIGQGGMATVYKAYQPSMD
                     RHVAIKVLPSRLAESKEFVTRFQQEARIIAKLEHPHILPVFDYGESDGVAYFVMRYLE
                     AGTLKERMVARRPLPLDEIDRIFTQLTDALSYAHGHGVVHRDLKPANALVDAQGNLFL
                     TDFGIAKLLESASPRLTQTDAIMGTPAYISPEQAQARTVDRRSDIYSLGVILYEMVTG
                     QVPFEAETPLAIILKHVSDPLPPPSLIKKDISQTLEQVILKALAKDPDDRFATAAEFL
                     AAWKRALNEEHEKATTYRTPQPIPAPAPRGGAPIPSTGTMSKPARTTGWVAGCLILAC
                     IALTVGGGGVLLASNWNMFSSATATIFPTSTSLPLPSETPIPIEFGNVLLEDDFSEEN
                     WGTLTDSDTVIKYVNSALNMQVFTNNWFVWSTPNDTDYENIHIEVTAIVNASDSNTAF
                     GVMCNQQASSDDSYYYFAITPAAQYAIAKSVVNQDGSILTNNDEWGTSTVITEDASSY
                     RIGADCGNGTLTLYVDGQKISSVSDSSYTGGGVALFTWSALEATTTDISFDDFYMTSL
                     P"
BASE COUNT         1305 a         1309 c         1233 g         1317 t
ORIGIN      
        1 ccgaattgat tcttattcct gcatcttgca tttgtttggc aagacttacc ttccaattcc
       61 cgattgaatc caaggggata taaacgaccc taaaagattc ggtcggtaat tctatactca
      121 aatcgggatt gctctcgaac aaagcgcaaa catgcttata ccctaattta ccacgaaaaa
      181 aacccaactc ataaatcaca tgggggctgg cgtgaaactt cgtaacgtcc gactccgatt
      241 ttgtcacgac cagatcgtcg gccgttagaa gtacaactgc aaatatgacc tccgtagcaa
      301 aaatatcgaa ttggcgatct ttgggccggt tcgatcttac gacttctaat ccaagtctta
      361 ctatataact tgcaactgca tcagcggcat ccatgttacg cccgtgtata agtaaaacct
      421 gattgagatc cttataagcg gaagtttcag aagtttggat tctccttggg gatgcacgtt
      481 cattcaacac gcgacgtgtt tgtctacgaa tctgctctgc agttacgcca agcctgcgta
      541 acacttcggt tgccacatca tccactagta caagtccaag taggatgtgc tcggtgccaa
      601 tataatgatg cccaagtcgg cgagcttcgt cgactgcaag ctcaagggct ttttgggttt
      661 gagatccaag ctcaacacga gcagggtcaa aattatcaga aacagtggtt attttctcga
      721 tggtttgacg aaggcgatct gctgacaacc ccaattcacg caaaacacga cctgctactc
      781 caccttccag catcaaaccg atcagaagat gttctgttcc aatgaagtta tttcgagctc
      841 gctcagtttc ctgatgtgcg aaactcaaca cactccttgc tctctgtgta aacctttcca
      901 tgccagccat aaaatctcct gacacccggc gaacgaaata ccccacacga tatttataac
      961 tcgacgaatc aatcctacat atctacgatc tcaccgccgt gttgaatggc agttgccacc
     1021 attccatcct gagggacatt cggtggaatt tttccctttg cagttgtcac aacacagcga
     1081 acatttacat ccacattaaa atgacttttg atcagcttct ggattgcttc gatctggtca
     1141 ggtttatcaa tctttgacac cagcacatca cttgccagcc ccaggatcaa cgttttgcct
     1201 tccatgtcga tcattcgaac cgagttcaac aatgccgaga gattggcctg agattttgga
     1261 agggagccag ataaatgctt ccataccttg atgacctcac ccgcacttat ctccggtttt
     1321 tgggactgat ttttacttgt agcgtcggtc gcttcaccag aaacgctgct cccttgcgcg
     1381 gacgattgtg tggattctgc agcatgtacg ggggtgggct gcggtctggc ctgacgcggc
     1441 gaggcagcca ggggcgctga ctctgccggt gcatccagca cttctgccaa agccaattcc
     1501 aaactcagag atggttgcca gccgccgcgc aggtccgttg cggcgttgtt gaaggctttc
     1561 atcatgcgca ggacatcgct tgtggtaaat gaacgggcgt gcgcctgcat ctgttttttg
     1621 acatctgccg tggcttcgac ttgattcgtg ttacccattt gaatgagcat cagcccacgc
     1681 agatattcga caatctgccg cgccagcgag cgcggatcgg cgcccgcatc caacgccttg
     1741 tgaatggact ccagcccatg cgcgggatca tgctcgttga ccgcgttgag cacatctagc
     1801 accgattgac tcgtggccgt gcccagcact tgttgcgcga gcgcaagcgt gatcttatcg
     1861 cccgtggacg aaagctggtc gagcagagat tgtgcgtcgc gcatcccacc tgccgactgg
     1921 cgcgcgattt gaatcaatgc atcatcatct gcctggatct tctcagcagc gacgattttc
     1981 ttcaggttag taacaatctc accaaccggc acgcgcctga actcgtgacg ctggcaacgt
     2041 gataagactg tcgcaggaat tttatgaatc tccgttgtgg ctaacacaaa gatggcatgc
     2101 ggtgggggct cttcaagcgt ttttaaaagt gcgttgaagg cggctgttga aagcatatgg
     2161 acttcatcaa taatataaat cttgtatttg ccctgtgagg gtgaaaagtt gatcttatcg
     2221 cgcaagtcac ggacgtcatc cacactggtg tttgaggccg cgtcgatttc aatcaggtca
     2281 aggaagcgat tctcgttgac ggctttgcaa ttttcacatt cattatcggg tcgtttggca
     2341 gggtcaggat tcgtgcaatt taccgctttt gccaggagac gagcgagcgt ggttttcccc
     2401 gttccgcgag agcctgcaaa tagataggcg tgggcaacgc gatctgctac gatagcattt
     2461 ttgagagtcg tgaccacgtg atcctggccg acgacggctt cccattcttt cggtctatat
     2521 tttcggtaga gggcttgggt cattttattt atacaccgtt ggttgagacg taaacagtcg
     2581 aaaccaacaa tttgaaatac tctgattatt tttttaactt tcgttatagg tggtttcgat
     2641 atggggcaga caaacacggc ccctactcaa ccaccgagga tgttatggca ctttatagac
     2701 ttcgcgtaaa tttccacttg ggtcaaacaa ttgagcttcc tcaccagact cccaaaccgg
     2761 ctcgctggca ctccagtata aatcaatgac ggtattggtt cccgaatccg tatgaacttg
     2821 tactgcacca ttcgtgtgaa gaacaagttg aggaaagaca aagacgttcc cgtcgtcatc
     2881 tctcaactgc cagtttgcga ggttgatctg gtcatcacct gtataactca cgaccaccca
     2941 ctccgcattc aaggttccag caccgatcac actgacaatt tccacaggga tatccgtttg
     3001 tggatcgagg gttgccgcag agacagcggc cgggttagag gatggcacgg agggcaatac
     3061 cggagaaaga ctcacagacc gatagttacg atggtaccaa aacaggatcg ctccagtcac
     3121 gcaggctgag atgaatacat taagaagtaa gtaaaagaaa agactgcggc gatccatgtg
     3181 aaatgaatca tagcacagat tacatctccc tgaattaaaa aatttgtacc tctcctgcag
     3241 aggcacaaat tttagaggca gaaccggatg tttatggtag cgacgtcata taaaagtcat
     3301 caaacgaaat atcggtcgtt gtcgcttcca atgcgctcca cgtgaataat gccacgccac
     3361 caccagtata ggaagaatca gaaaccgaac tgatcttctg tccatccaca tataaggtta
     3421 acgtgccatt tccgcagtct gcaccgatgc gataggaaga ggcatcctcg gtaatgacag
     3481 tcgatgtacc ccattcatcg ttattggtca ggatcgaccc atcctgatta acgacagatt
     3541 tcgcaatcgc atattgtgcc gccggtgtaa tggcgaaata ataataagag tcgtccgaag
     3601 aagcctgttg attacacatc accccaaacg ccgtatttga gtcggaggcg ttgacgattg
     3661 cagtcacttc aatgtgaata ttctcgtaat cagtatcgtt cggcgtgctc cagacaaacc
     3721 aattgttcgt aaaaacctgc atgttcaaag cactattcac atatttgatc actgtatctg
     3781 agtcggtaag cgtgccccag ttctcttcag agaaatcatc ctccagaaga acatttccga
     3841 actcgatggg aatcggagtt tcagacggca aaggaaggct tgtcgaagtc gggaaaatag
     3901 tcgcggtagc cgaactgaac atattccaat tcgaagcaag caggactcca cctcccccaa
     3961 ccgtaagcgc gatgcacgca aggatcagac agcccgcgac ccagccggtg gtcctggcgg
     4021 gtttggacat ggtcccagtc gaggggatcg gtgctccgcc gcgcggtgca ggcgcaggta
     4081 tcggctgagg tgtgcggtag gttgtcgcct tttcgtgctc ttcattcaga gcacgcttcc
     4141 aggctgctaa aaattcagcg gcagtggcga atcgatcatc gggatccttg gcgagcgcct
     4201 taagaatgac ctgctcaaga gtttgagaaa tatctttctt aattaaagaa ggcggcggca
     4261 gtggatcact gacatgttta aggataattg ccagtggagt ctccgcttca aaaggcactt
     4321 gaccggtcac catttcatag agaataacgc cgagcgagta aatatccgag cgccggtcta
     4381 cggtgcgtgc ctgagcctgt tcagggctaa tataagcggg cgtgcccata atggcatcgg
     4441 tctgtgttaa gcgcggagag gcgctttcca gtaacttcgc gataccaaag tcggtgagaa
     4501 agagatttcc ctgtgcatcc accagtgcat ttgcaggttt caggtcgcga tggaccacac
     4561 catgcccatg tgcatagctt aacgcatcag tcaattgtgt gaagatacga tcgatctcat
     4621 ccagcggaag tggacgtctg gcgaccatcc gctctttaag cgtccccgcc tccaaatagc
     4681 gcatcacgaa ataggcaacc ccgtcgcttt ccccataatc gaagaccggc agaatgtgtg
     4741 gatgttcaag cttcgcaata atgcgcgctt cctgttgaaa ccgcgtgaca aactctttgc
     4801 tttcggcgag ccggctcgga aggactttta tggctacatg acgatccatg gaaggttgat
     4861 aggctttgta gaccgtagcc ataccaccct gaccaatttg attgataata cgatacgagc
     4921 cgagcatttg cccgggttgc aggttatcca tgtcgcgctc ctgggaatca gacatttcta
     4981 ccgcctgtct cattaattac caaacaatta ccaaactctt gaaaatggct tgtgtgccaa
     5041 tattcaaagt tgaagttcag gtaatcatgc ggcactctgt gattgctcca ttttacaacc
     5101 gtttgattcg aaatttattg cgatttaacc acagaacagt tcgacgttgt tgcggttgcc
     5161 ttct
//
LOCUS       DASTJP010000144         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_418974, whole genome shotgun sequence.
ACCESSION   DASTJP010000144 DASTJP010000000
VERSION     DASTJP010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418974"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..248
                     /locus_tag="VFQ23_04315"
     CDS             102..248
                     /locus_tag="VFQ23_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905833.1"
                     /translation="MNRTLSHPFGYARLVQSGTLLDQGTNGLTIVRDGKIIFYGTEDT
                     EPVQ"
     gene            complement(326..1543)
                     /locus_tag="VFQ23_04320"
     CDS             complement(326..1543)
                     /locus_tag="VFQ23_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905834.1"
                     /translation="MESNNGRWGEEFARLALAIDQHLPGYVDSYFGPNEWMAGAKQEG
                     KLPLSDLTKRVNQLATDISQVDDLDAQRRDFLARHVNAMQMSLRLLDGEKISLSEEVR
                     ALYDVQPAWKDELIFVEAHQELDLLLPPGNSLPERMQDWNRSLEIPVEKVKELLPVIV
                     QRLQQLAHQKFNLPEAESFMVEFVSNQPWGAYNWYLGKYKSRIDFNTDLPAKVDRLAD
                     LMAHEGYPGHHTELSIKETKLIEQKNYYEHTLTLINSPSCVVSEAIATTALETILTKA
                     ELEDWYREEILPLAGLASIDAKRLMEVNAAKVKMSGLAGNAAFMLHDQHTDASEISLY
                     IQKYGLNTETEAGQRIKFISHPLYRSYIFTYHIGYDLLKELFLNVDRETYFKRLLEEP
                     VTPHQIREWIKNN"
     gene            complement(1641..2702)
                     /locus_tag="VFQ23_04325"
     CDS             complement(1641..2702)
                     /locus_tag="VFQ23_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9905835.1"
                     /translation="MGRRRTIGYIQNEWTCPNCDTRNKGGAKTCENCGAPQPENVQFE
                     LPSEQKLVTEQDQIKAAQGGADIHCPFCGTRNPADAQTCSQCGGDLVEGKAREAGRVM
                     QAPPPQPKVVKCDNCGVENPGSNSLCSNCGSPLPKVSALQSTPVRPAAGARPAMPLSA
                     PKKTNWLLIGGIIAALAICCIAALALFLPSKSVEATVVDVHWQTSVPVQEIQAVSHSD
                     EPGSPPSDAYNVSCYDDSRDVCEQRTIDKGNGYSEVVEECHTETQQYCSYTVDEWTTI
                     QTYPMEGNDLRPIYESPNIASDQRLGNASENLTVVFSTSNGEETYSPNSVSEFQQYSI
                     GSTWTLRMNAIGNVVSVEP"
     gene            complement(2746..>3251)
                     /locus_tag="VFQ23_04330"
     CDS             complement(2746..>3251)
                     /locus_tag="VFQ23_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905836.1"
                     /translation="DERAAFLSVHQFQPGFYPGTGALTDAPHAKKRIVNVPLPARAGD
                     QVYERVADKIFTPFVETIKPQLLFISVGFDAHWNDPITMLGLSTKGYLTLAQKVIALA
                     EGHCDGKIVFVLEGGYDPTNVANGAEAVFVAATGKGESEVSDPNPYQEPDCEARIEEV
                     RKWHGFS"
BASE COUNT          767 a          828 c          783 g          873 t
ORIGIN      
        1 acaacgaaga atcggcgtcg ctgagataat cctgtggatg gatggaaacg aaaggttggt
       61 atgctataag aatgcgaaga ccaacgcagc ggagaagccg gatgaatcgg actctttctc
      121 acccatttgg ctacgcgcga ttggtacaga gtgggaccct gctcgatcag ggcacgaatg
      181 gactgacaat cgtcagagat ggaaagatca tcttctacgg cacagaagat actgaaccgg
      241 ttcaataaaa atcagtgggc attaaagaag acatgcacct tgaaatgtgc atgtcttctt
      301 ttcgttcgat tgcctgataa aacagttaat tgttcttgat ccattcacga atttgatgcg
      361 gggtaaccgg ctcttccagt aatctcttaa agtatgtctc gcgatcgaca tttaaaaata
      421 attccttcaa gagatcataa ccaatatgat acgtaaaaat gtatgatcga taaagtgggt
      481 gggagatgaa cttgatccgt tgtccggctt ccgtttctgt gtttaagcca tatttctgta
      541 tatagaggct gatttcactt gcatctgtat gctggtcgtg cagcatgaac gccgcatttc
      601 ctgccaggcc agacattttc acctttgcag cgtttacttc cattaacctt ttagcgtcaa
      661 tcgaggccaa tccggcgaga ggcaagatct cttcccgata ccagtcttca agctcagctt
      721 ttgtcagaat ggtttctagt gcagttgtcg caatcgcttc tgaaaccaca cacgatggag
      781 agttgattaa ggtcagggtg tgctcatagt aattcttttg ttcgattaat tttgtttcct
      841 tgatcgaaag ctcagtatga tgaccaggat acccctcatg cgccatcaga tctgccagtc
      901 tatctacctt agcaggcagg tctgtgttga agtcaattcg agatttatac ttccccagat
      961 accagttata cgcgccccac ggctgattcg agacaaactc caccataaac gattcagctt
     1021 ctggcaggtt gaatttttga tgtgcaagtt gttgcaacct ctggacgatc acggggagta
     1081 attccttaac cttttcaacc ggaatttcaa gagaacggtt ccagtcctgc atgcgctcgg
     1141 gtaaagaatt gcctgggggc aggagcagat ccagctcttg atgagcctct acaaaaatca
     1201 attcatcctt ccatgcaggt tgaacatcgt acaaggcgcg cacttcctca gaaagtgata
     1261 ttttttcacc gtccaacagg cgaagactca tttgcatggc attcacgtgc cgtgcaagga
     1321 aatctctgcg ctgcgcgtcc aaatcgtcta cttgtgagat atctgttgca agctggttga
     1381 ctcgtttcgt tagatcagag agcggcagtt ttccctcctg ttttgctcca gccatccatt
     1441 cattcggtcc aaaataggaa tcgacataac caggcaggtg ctgatcgata gccaatgcca
     1501 gccttgcaaa ttcttcgccc caacggccat tgtttgattc catttcctat cctttcccta
     1561 aagctatcca gagtatctgt acgatatgtg cccctcttta atacctatcg cgtttttcaa
     1621 gattatgtga cacgtatgcc ttacggttcc acactgacca catttccaat ggcgttcatc
     1681 cgcaacgtcc atgtgctccc aatggagtac tgttggaact cagagaccga gttgggtgaa
     1741 taagtttctt cgccgtttga cgttgagaag acgaccgtca aattctcaga tgcgtttcca
     1801 aggcgttgat cgctggcaat attcgggctt tcataaatcg gacgcaggtc attgccttcc
     1861 atgggatagg tctgaatggt ggtccattca tctactgtat aagaacaata ctgctgggtt
     1921 tccgtatggc attcctcgac aacttcggaa taaccgttgc ccttatcaat ggtccgttgc
     1981 tcgcagacat cccggctgtc gtcatagcaa gagacgttat aggcgtccga tggcgggctg
     2041 cccggttcgt cagaatgact aaccgcctgg atctcctgca ccgggaccga ggtctgccag
     2101 tgtacatcga cgaccgttgc ctcaaccgat ttggatggta aaaacaaggc cagcgccgcg
     2161 atgcaacaga ttgccagcgc tgcaatgatt ccgccgatca acagccagtt tgtctttttc
     2221 ggtgcagaaa ggggcatagc gggtctggca cccgccgcag gtctgaccgg tgtggactgc
     2281 agcgctgata cctttggcag gggcgatccg caattcgagc agagagaatt gctcccgggg
     2341 ttttcaactc cacagttgtc gcatttcacc accttgggct gcggcggcgg cgcctgcatc
     2401 acacgcccgg cttcacgcgc tttaccttct accaaatccc caccacattg agagcaggtc
     2461 tgggcgtcgg cgggattccg cgtgccacaa aatggacagt ggatatcggc gcctccctgt
     2521 gcggctttga tctgatcctg ctcggttaca agcttttgtt ctgacggaag ttcaaactgc
     2581 acattctctg gctgtggcgc gccacagttc tcgcaggtct tggcgcctcc cttgttgcgt
     2641 gtgtcacagt ttggacaggt ccattcattt tgaatgtaac cgatcgttcg tctgcgtccc
     2701 ataattgctc ctgattgcta cctacaaata attataaaag atggcttacg aaaatccatg
     2761 ccacttgcgt acttcttcaa tgcgtgcttc gcaatccggt tcctgatagg gatttggatc
     2821 gctcacttca gattcgcctt tgccggtggc agccacaaaa accgcttctg ccccgttcgc
     2881 gacgttggtt ggatcataac cgccttcaag cacaaaaaca atttttccat cgcagtgtcc
     2941 ttccgccagc gcgatcactt tttgtgccag tgtgagatat cccttggtag ataagccgag
     3001 catggtgatc gggtcattcc aatgcgcgtc aaaaccgacg gagataaata agagctgcgg
     3061 cttgattgtt tctacaaaag gagtaaagat tttatctgcc acccgctcgt agacctggtc
     3121 ccctgcgcgg gccggcagcg gcacattgac gattcgcttt tttgcatgag gggcatcggt
     3181 caaggcgccc gttccggggt agaaacccgg ttggaactga tgtactgaca aaaacgcggc
     3241 gcgctcatca t
//
LOCUS       DASTJP010000145         8194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_912701, whole genome shotgun sequence.
ACCESSION   DASTJP010000145 DASTJP010000000
VERSION     DASTJP010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912701"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFQ23_04335"
     CDS             <1..280
                     /locus_tag="VFQ23_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905837.1"
                     /translation="SKNGIQSSAEYYEFLELCSDAEDRINSGGTGMGYSGIHYLVMHK
                     LKQYGRFANGREDSIRVLKQLLKEYELENNINKAQSDDEYLNKFDDPE"
     gene            511..1557
                     /locus_tag="VFQ23_04340"
     CDS             511..1557
                     /locus_tag="VFQ23_04340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9905838.1"
                     /translation="MLTKPDIPDELIISRLQEEYGLQVASLTFLPLGADMGTAVYRVV
                     GDDGTANFLKLRKGFDEITVKVPLFLKSQGIKEIISPIETKSKRGWADFGEYKMILYP
                     FIDGKNGFEIELTDHQRRILGAALKAIHSAQIPPELKTQIPQEMFSPLFRENMKSFQE
                     QVESLTYDEPVAAKLAEFIKLRQDEITQLIARAEQLLSEVQSKPLDFVLCHTDIHGGN
                     ILIGMDGQLAEHPDGVLVPTSNIYIVDCDAPLLAPKERDLMFIGGGIDEIWKSKQDES
                     VFYDGYGKTEINLAALAYYRYERIIQDLVAFCEQLLLSDEGGSDREQAYRWFTSNFEA
                     GSTIEIADSTYRKI"
     gene            complement(1601..2518)
                     /locus_tag="VFQ23_04345"
     CDS             complement(1601..2518)
                     /locus_tag="VFQ23_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9905839.1"
                     /translation="MKYIKVPNTGMNASEIVLGCMRISSMTNQEISTLVNTAMDEGIN
                     FFDHADVYGGGKCEEKFSEALGMNASLREKMILQTKVGIRKGSFDFSKEHLLNAVDGS
                     LKRLRIDYLDVVLLHRPDALVEPEEVAEAFDILESSGKVKYFGVSNQNPMQIELLTKF
                     VRQKIYFNQLQLSITNTGMIDAGINVNMKIDRSIDRDGSILDYCRLKDIIIQPWSPFQ
                     FGFFEGVFLDNPKFPALNQKINAIAADKGVTNTAIAIAWLLRHPAHMQPILGTTSPQR
                     VKDSCQASGITLCREEWYQIYLAAGNQLP"
     gene            complement(2588..3406)
                     /gene="trpA"
                     /locus_tag="VFQ23_04350"
     CDS             complement(2588..3406)
                     /gene="trpA"
                     /locus_tag="VFQ23_04350"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET9905840.1"
                     /translation="MSRIENAFKNKPIFMPYFPLGYPDLSTSIDVIEALAKNGADLIE
                     VGLSFSDPLADGPVIQKATQVALEQGITVKKSLEAVKELRNRGVEIPLVLMGYYNPML
                     AYGLEKFVRDSVSAGADGFIVPDLPIEESDEFESALDSVGGRVDGGDVLPSYRDYPLI
                     QMLAPTSSPVRMEMIARNAQGFIYLVSVTGVTGERKSISDGLGELISRVREHTSAPVC
                     VGFGIGTPEQAKEVGKMADGVIVGTACVRTIGGSESPVETAKQFAAEFRSALNE"
     gene            complement(3591..4790)
                     /gene="trpB"
                     /locus_tag="VFQ23_04355"
     CDS             complement(3591..4790)
                     /gene="trpB"
                     /locus_tag="VFQ23_04355"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET9905841.1"
                     /translation="MSATLLPHKFGPYGGQFVPETLMPALIELEEAFVSARSDLAFQD
                     EFNKLMASFVGRPTPLTYAKRLSEKLGGAQIYLKREDLAHTGAHKINNALGQALLVKR
                     MGKARIVAETGAGQHGVASATAAALLGLECVVYMGVVDIARQEPNVFRMKLLGAEVRP
                     VTSGTQTLKDAINEAIRDWVTNVRGTHYLLGSALGPHPYPTIVREFQSVIGSEAREQM
                     MMDVGRLPDTVIACVGGGSNAIGVFSGFVKDEQVELIGVEAGGSGIETGKHAARFGDP
                     SKGRVGVIHGTRTYVLQDEDGQIAETHSVSAGLDYAAVGPEHALMRDNERAFYTSATD
                     EEALNAFQTLCHTEGIIPALESSHAVAEVIKRAPTMRKDQVILVNLSGRGDKDLNTVI
                     KELGTEI"
     gene            complement(4848..5474)
                     /locus_tag="VFQ23_04360"
     CDS             complement(4848..5474)
                     /locus_tag="VFQ23_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET9905842.1"
                     /translation="MTKIKICGIKTLDGALAAIDAGADYLGFNFYPKSPRFIEQQTCA
                     DVTSVLKKAHPHIKLVGVFVNSSVEEIKEILDLCSLDLAQLHGDETVEFMESLPEKSF
                     KAFRGIPANVNGFARVEPPALLVDASVKGVYGGSGVTADWDAAAELAKKYPVLLAGGL
                     TPENVADAVRRVKPWGVDVASGVESAPGEKDAGKMVEFVKAVRVAESH"
     gene            complement(5485..6339)
                     /gene="trpC"
                     /locus_tag="VFQ23_04365"
     CDS             complement(5485..6339)
                     /gene="trpC"
                     /locus_tag="VFQ23_04365"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET9905843.1"
                     /translation="MSILEKIIEHKKTEIAALDSQALRRAVDSSSAPRDFLAAISPLH
                     TGVGLGVKVNRISGSPSLIAELKRASPSKGILAPHLDLFQVADIYTQNGASAISVLTD
                     EKFFLGKLDTLRELRFERKSELPLLRKDFTIDEVQLYEARANGADAILLIAAALTDEA
                     LFADLHACALSLGLTALVEVHDEAETERALKLKDVRLIGINNRNLATFHVSLETTERL
                     RPMIPPEIAVVAESGIFTASDVERLAKANVDAILVGEALVTSEDIPAKVRELSGARVP
                     SPSGRGLG"
     gene            complement(6336..7952)
                     /gene="trpD"
                     /locus_tag="VFQ23_04370"
     CDS             complement(6336..7952)
                     /gene="trpD"
                     /locus_tag="VFQ23_04370"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET9905844.1"
                     /translation="MLVLIDNYDSFTYNLVQYFGELGADIKVFRNDQVTINQLIALNP
                     SHLVISPGPGEPIKDDGISSDAIKHFTGKIPVLGVCLGHQALGAVFGGKVDRAQRLMH
                     GKTSKVTHNGQGIFKGVPSPFEAMRYHSLVVYDPIPSELEVTAITPEEEIMGLKHREH
                     QTYGVQFHPESILTQHGKQILKNFLDLNPAPAKGEQAMLKPFIAKAINRTDLTDAEAE
                     EAMNIIMTGQATPAQIGAYLVALRMKGETIPEITGSVRAMRTNAVKVKLNTDEPVYDI
                     VGTGGDGAHTFNISTAAAFVLAGTGRKVAKHGNRAASSQCGSADVLSALGVNLDLTAE
                     QVAQAIEQVGIGFMFAPKFHPAMKHAIGPRKEIGQRTIFNILGPLTNPAGANVQLTGV
                     FDPKLTEPLAYVLKELGSKAALVIHGANGLDELNTTGANRVSHLKNGAVETYDLNPSD
                     LGLGASTVQDLRGGTPDESAMMMRELLSNKLNGARRDAILLNAAAALAAETGDFNSAL
                     EEAKASMDNGSALAKMNALVEFSQSFQTIQ"
BASE COUNT         2009 a         2224 c         1946 g         2015 t
ORIGIN      
        1 ctcaaagaat gggattcaat cgtcggctga gtattacgaa tttttggaac tgtgcagcga
       61 cgctgaagat cgcattaact ccggcggcac agggatgggt tacagcggca tccattattt
      121 ggtcatgcac aagctgaaac agtatggcag gtttgccaac gggcgggaag actcgatcag
      181 agttctcaaa caattgctca aggaatatga actggaaaat aacatcaaca aagctcagtc
      241 cgatgatgaa tatctgaaca agtttgatga ccctgaataa caaggaaacg cccgccattg
      301 ggtgtttttt ttatatcact tatgcctcgt tggttgagta agcgtccatc gtccgccgta
      361 cacctgcacc caccgcaggt gcagtgtcga aaccaacagg ctattgaaag gaatctctta
      421 acagagatta tccacttatt ggtggtttcg atacaacctg cgacgatcac gcaggtctac
      481 tcaaccgccg gtttggattg aaaattttta atgctcacga aacccgacat cccagacgaa
      541 cttatcattt cccgattgca agaagaatat ggattgcaag ttgcctcatt gaccttcctc
      601 ccgctcggcg cggacatggg caccgccgtc tatcgtgtcg tcggggacga tggaacggca
      661 aacttcctga aattgagaaa aggctttgac gaaatcaccg ttaaagttcc gctctttctc
      721 aaatcacagg gaataaaaga aatcatttcc ccaattgaaa ccaaatcgaa acgaggctgg
      781 gcggacttcg gcgaatacaa gatgattttg tatcccttta tcgacgggaa aaacggtttt
      841 gaaatcgagc tgactgatca ccagcggcga attttaggcg cggcgctcaa agcgattcac
      901 tccgcacaaa tcccacctga gctgaagacg cagattccgc aagagatgtt ttcacctctc
      961 tttcgcgaaa atatgaagtc ttttcaagaa caggtggaaa gcctgaccta cgatgagcca
     1021 gtcgcggcga aattagccga attcattaag ttgaggcagg acgagataac ccagcttatt
     1081 gcacgcgcgg aacaacttct ttcagaggtt caatccaaac cgttggattt cgtcctttgc
     1141 cacacggaca ttcacggcgg taacatcctt attggtatgg atgggcagct tgcggagcac
     1201 cctgacgggg tgctcgtccc tactagcaat atttacatcg tggattgtga tgcacctctc
     1261 cttgcgccaa aagaacgtga cctgatgttc atcggcgggg gcatcgacga aatctggaag
     1321 agcaaacagg atgaatctgt tttctatgat ggatatggaa aaacagagat caatctcgcg
     1381 gcgctggcat attaccgcta cgaacggatc atccaagacc tggtcgcgtt ttgtgagcaa
     1441 ctgctattaa gcgacgaagg cggatcagat cgggaacaag cctaccggtg gttcacgtcc
     1501 aattttgagg caggtagtac gatcgagata gcggacagca cttataggaa aatataaatt
     1561 ctgccatgga taaatccacg gcagaatagt tctacgccat ttacggcagc tgatttcccg
     1621 ccgccaaata gatctgatac cactcctcgc gacatagggt aatcccagat gcctggcagg
     1681 aatcctttac tctttgtgga ctcgtcgtgc caaggatcgg ctgcatgtga gcgggatggc
     1741 gcaggagcca cgcaattgca atagcagtat tcgtcacacc cttatcggct gcaatcgcat
     1801 taatcttctg gttgagtgcc gggaacttcg ggttatccag gaatacgcct tcaaagaagc
     1861 caaattggaa tggcgaccag ggttgaatga taatatcctt caagcgacag taatccagga
     1921 tgctgccatc ccggtcgatg gaacgatcaa ttttcatatt cacattgatc cccgcatcga
     1981 tcatgccggt attggtgatg ctgagctgaa gctggttgaa gtaaatcttc tgcctgacga
     2041 atttggtcag caactcgatc tgcatcgggt tctggttgct gacgccgaaa tatttcactt
     2101 tgccgctgct ttccaaaata tcaaatgcct cggcgacctc ttcaggctct accaatgcat
     2161 caggacgatg cagcagaacc acatcaagat agtcgattct caaacgcttc aaacttccat
     2221 cgaccgcatt caacaaatgt tctttggaaa aatcaaaact tcctttacga atgccaacct
     2281 tggtttgcag aatcatcttc tcgcgcagac tcgcgttcat acccagcgct tccgaaaatt
     2341 tctcttcaca ttttccaccc ccatacacat cggcatggtc aaaaaaattg atgccttcat
     2401 ccatggcagt gtttaccagc gtggagattt cctgattcgt catgctggaa atccgcatac
     2461 aacccagcac gatctccgat gcgttcatgc ctgtattcgg tactttaatg tatttcattg
     2521 aaacacctct ggttgggatc tgggattttg gtatcggttc gcgctggcgg aagttatacc
     2581 atagctgtta ttcgttcaaa gcacttcgga actcggccgc aaactgtttc gccgtttcaa
     2641 ccggactttc acttcctccg atcgtcctca cgcaagctgt tccgacaatc acaccatccg
     2701 ccatcttacc gacttctttg gcttgctctg gtgtgccgat tccaaacccc acacagacgg
     2761 gcgcggaggt gtgctcacgc acgcgcgaaa tcaactcgcc gagtccatcc gaaatggatt
     2821 tgcgttctcc cgtcacaccc gtcacggaga cgagatagat aaatccctgg gcgttgcggg
     2881 cgatcatttc catgcgtacc ggagaagaag tcggtgcgag catttgaatc aacgggtagt
     2941 ctcgataaga cggcagaaca tcacccccgt ctactcgacc accgacacta tcaagcgcgg
     3001 attcaaattc atcggattcc tctatcggca gatcggggac aataaatcca tccgcacccg
     3061 ccgataccga atcacggaca aatttttcca atccatacgc caacataggg ttgtaatagc
     3121 ccatcaacac cagcggaatt tccacaccac gattgcgcaa ttccttcaca gcctccagcg
     3181 acttcttcac cgtaatcccc tgctccaacg cgacttgcgt ggctttttga atcacaggcc
     3241 catccgctaa cggatcagaa aaggacaaac ccacctcgat caagtccgcg ccgtttttag
     3301 caagcgcctc gatcacgtca atggatgtgc ttaggtcagg atagcccaat ggaaagtaag
     3361 gcatgaagat gggtttgttt ttgaaggcgt tttcgattcg gctcataata attcctattc
     3421 aaaagcaatt cggtggtcga atagagcgat tgtttttctc gctcgtatcg aaactacaaa
     3481 tcagcaaaat aatccctatg ggtgctggtc tcgatatgcc ttcggctact cgaccaacga
     3541 ttggttcttc atctttcttc tttctttgcg accttagcgt gctttgcggt tcatatctcc
     3601 gtgcccaatt cctttatcac ggtattcaaa tccttatccc ctcgccccga taaattcacc
     3661 aaaattacct gatcctttct cattgtcggc gcgcgcttga tgacctccgc taccgcatgc
     3721 gatgactcca acgccgggat gattccttcc gtgtggcaca gtgtttgaaa ggcgttcaat
     3781 gcttcttcat ctgtggcaga tgtgtaaaac gcgcgttcgt tatcccgcat cagtgcgtgt
     3841 tccggaccga ccgctgcata atccaacccc gccgagacgg aatgcgtctc cgcgatctga
     3901 ccatcctcat cctgcagcac atatgtccgt gtgccatgga tgactcccac gcgtcccttt
     3961 gacgggtcgc cgaaacgagc tgcatgttta ccggtttcga ttccactacc acctgcctcg
     4021 acgccgatca actccacttg ttcatccttc acgaacccgc taaagacacc gatcgcgttc
     4081 gacccgccac ccacacatgc aatcaccgta tcgggcaatc gtcccacatc catcatcatt
     4141 tgctcacgcg cttcactccc aatcaccgac tggaactcgc gaacgatcgt ggggtatgga
     4201 tgaggtccca gcgccgaacc gagcaggtaa tgtgtaccgc gcacgttcgt aacccagtca
     4261 cggatcgctt cgttgatcgc atccttcaag gtctgtgttc ccgatgtaac aggtcgcact
     4321 tctgcgccga gcagtttcat ccgaaatacg ttcggctcct gccgcgcaat atctaccaca
     4381 cccatataca ccacacactc gagccccagc aacgccgctg cggttgcgga tgcgacccca
     4441 tgctgccccg cgccggtctc tgctacgatg cgtgccttgc ccatccgctt gacgagcaag
     4501 gcttgcccca acgcgttgtt gatcttgtgt gcgcctgtat gcgccaaatc ttcgcgcttc
     4561 aaataaattt gcgcaccccc caatttttcc gagagacgtt ttgcataggt taagggagtc
     4621 ggtcggccca cgaatgacgc catcaacttg ttgaactcat cctgaaacgc gagatcgctt
     4681 ctcgctgata cgaacgcctc ttccaactca atcaaggcgg gcattaacgt ttcggggacg
     4741 aattgtcctc cgtacggacc gaacttgtgg ggtaaaagtg ttgctgacat aatatctctt
     4801 ctttggaaag tggaaagcag aaacctgact ctttcacttt ctactttcta atgggattct
     4861 gcgactctaa cagcctttac aaactcaacc atctttcccg catctttctc ccccggagcg
     4921 gattccacac ccgaagccac gtccacaccc cagggcttga ctctgcgcac tgcatccgcc
     4981 acattctccg gagtcaaccc gccggccaac aacaccggat acttcttcgc caactctgcc
     5041 gcggcatccc agtctgcagt gactccactc ccaccataca cgcctttgac tgaagcatcc
     5101 actaacaatg cgggaggttc aacccttgca aaaccattta cattcgcagg gattccacga
     5161 aaagccttga acgatttctc tggaagagat tccataaact ctaccgtctc atcaccatgc
     5221 agttgtgcca gatcaagcga acaaaggtca agaatttcct ttatttcctc aacggacgaa
     5281 ttgacaaaca caccaacgag cttgatgtgc ggatgtgctt tcttcaaaac agatgtaacg
     5341 tcagcacaag tttgctgctc aataaatctt ggactctttg gataaaaatt gaaacccaaa
     5401 taatctgcgc ctgcgtcaat cgcagcgagg gcaccatcaa gagttttgat tccgcagatt
     5461 tttattttgg tcaccacttc tccctcaccc tagccctctc ccgctgggag acgggactcg
     5521 tgcacctgaa agttccctca ccttcgcagg aatatcctct gaagtgacca acgcttcgcc
     5581 aacgagaatt gcgtcaacgt ttgcctttgc caatcgttcc acatcgctgg cagtaaaaat
     5641 tccactttcg gcgacaacag caatctcagg cggaatcatc gggcggagtc tttctgttgt
     5701 ttcgagcgag acatggaacg tagcgagatt gcgattgttg ataccgatca atcttacatc
     5761 cttcaacttc aacgctcgtt ccgtttcggc ttcgtcgtga acttcgacaa gagcagtcaa
     5821 acccaggctc aaagcacagg catgcaaatc cgcgaagaga gcctcatcgg tcaaggcggc
     5881 ggcgataagg aggatggcgt ccgctccgtt cgcgcgggct tcatagagct ggacctcgtc
     5941 gatcgtgaaa tcttttcgga gcaacggcaa ttcggatttg cgttcgaagc ggagttcacg
     6001 cagagtatca agtttgccga ggaagaactt ttcatccgtc agcaccgaga tagcagacgc
     6061 gccgttttgg gtgtaaatat ccgcaacctg gaatagatca agatgaggtg caagaatgcc
     6121 tttggaagga gatgcacgtt tgagttcagc gatcaagctg gggcttcccg agatgcggtt
     6181 caccttcacc cctaacccca ctcccgtgtg gagaggggaa atcgcggcaa gaaaatctct
     6241 aggtgcagaa gaagagtcca cagcccggcg taaggcttga gaatcgagcg ccgcaatctc
     6301 ggtctttttg tgttcgatga ttttttcgag aatgctcatt gaatagtttg gaaactttgg
     6361 gagaattcaa ccagcgcatt cattttagcg agggcactac cattatccat agaggccttc
     6421 gcttcttcta gtgcagaatt gaagtcacca gtttcggcag cgagggctgc cgcagcgtta
     6481 agcaggatgg cgtcacggcg cgcgccgtta agtttattcg agagaagttc acgcatcatc
     6541 atcgctgatt catctggggt accaccgcgc agatcctgaa ccgtggaagc accaagacca
     6601 aggtcactcg gattgagatc atacgtttcg accgcgccgt tcttgagatg actgacccgg
     6661 ttggccccag tcgtgttgag ttcatcgaga ccattcgccc cgtgaatgac gagagcagcc
     6721 ttggagccaa gctctttgag aacgtatgcc agcggctcag ttagtttagg gtcgaaaaca
     6781 cctgtgagtt gaacgttggc acccgccggg ttcgtaagcg gaccgagaat gttgaatatc
     6841 gtacgctgac caatttcctt gcgcggacca atggcatgct tcattgccgg gtggaacttg
     6901 ggcgcgaaca tgaatccgat accaacctgc tcaatggctt gagccacctg ttcagcagtg
     6961 aggtcaaggt tgactcccaa agccgagagg acatctgcac ttccacactg ggaagaggcg
     7021 gcgcggtttc catgcttggc aactttgcgt cccgtccccg ccaggacgaa cgccgcggcc
     7081 gtcgagatgt tgaacgtgtg cgccccgtca ccgcctgtac ccacgatatc gtagacgggt
     7141 tcatctgtat tcagttttac cttgaccgca ttggtacgca tcgcacgaac ggagccggta
     7201 atctcaggaa tggtctcccc tttcatccgc agggcaacaa gataggcacc gatttgcgca
     7261 ggcgtagctt ggcccgtcat gatgatgttc atcgcttctt cggcttctgc atccgtgagg
     7321 tcagtgcgat tgatggcttt ggcgataaat ggttttagca tggcttgttc tcctttggcg
     7381 ggagcaggat tcaaatcaag aaagtttttg aggatttgtt taccgtgttg ggtcagaatg
     7441 gactcggggt ggaattgcac accatacgtc tgatgttcgc ggtgtttgag tcccatgatc
     7501 tcttcttcag gcgtaatggc agtgacctcg agctcggatg ggatcgggtc atagaccacc
     7561 agcgagtgat agcgcattgc ttcaaacggc gaaggaacac ccttgaaaat tccctgccca
     7621 ttgtgagtca ctttggacgt cttgccatgc atgagacgct gagcgcgatc gaccttgcca
     7681 ccaaagactg cacccagggc ctgatgcccg agacagactc cgagcacagg gatcttgccg
     7741 gtaaagtgtt ttatagcatc cgaagaaata ccatcatctt tgatcggttc acccggacct
     7801 ggcgagatga caaggtgcga cggattcagc gcaatgagct gattgatcgt gacctggtca
     7861 ttgcggaaga ctttgatatc cgcgccaagt tcgccgaaat actgaacgag gttgtaagta
     7921 aacgagtcgt agttgtctat aagtacgagc atataaactt ccttttatgt gtcattgcga
     7981 gcgagccgta taggcgagcg tggcaatctc atgttaggta gttagttgag attgcttcgg
     8041 gcttcgccct cgcaatgaca ttactaatca tactctccaa agatatccac ctgatccaca
     8101 aacaaatcaa gcgaataaga gagaccggtc ttctcgcgga cgagttccat cgttgccttg
     8161 gcttcggctt gcatacgccg gattccattg gcga
//
LOCUS       DASTJP010000146        15621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1138474, whole genome shotgun sequence.
ACCESSION   DASTJP010000146 DASTJP010000000
VERSION     DASTJP010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138474"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VFQ23_04375"
     CDS             <1..1676
                     /locus_tag="VFQ23_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9905845.1"
                     /translation="HRKTLNALQESSVTRESMTRLAHHAEAIHDPDAVLEFAPLAAWW
                     ASTTSSHREAVALYELALRFAKSLPPANHAQMLEAYMVELEFMNRFSDEIEACRSAIA
                     LWHSIGNRLREGKNLATLAGLCMNVGWRSEAEKSIERAITILEDLSPSAELAQAYTEQ
                     CYIRMMYRDCEAALPWGEKAIKLAERFEDFDALARAYDYMGCAALIIDYDRGLKLLER
                     SLVIAREKKLPFAVGGTLGNLGGMLLEVREVTLANQYLAESINYARQQDNDYHLMESQ
                     AWQALANMYQGKWIEADTVARKILEHSSNLPNVTRASVLFTLGRIRIRQGGNNVDQLL
                     DEAFSVSIQADAPRFDSPRLLQAEFYWLIGNQPRVLEETHTAYELALRNKHPWIAGEL
                     AFWRWRSGDKNSPPAWIAKPFALQIAGDWHAAAKEWEERGCPYEQAMALVDGDDAAQL
                     AALDIFERLGALPALDILKRKMRADGTRIPRRPRPATRENPFNLTAREMEALVCLVDG
                     LSNHAIAKKLSLSTRTAEHHIASILQKMGVGSRSEAVALALKENLLRTE"
     gene            1832..2026
                     /locus_tag="VFQ23_04380"
     CDS             1832..2026
                     /locus_tag="VFQ23_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905846.1"
                     /translation="MDKYYFYHQLAQEHQYEVSKELANRHLLRNGKEYPFTAKQARRL
                     VWRAAFAVVTFILLAFYMIG"
     gene            2162..2638
                     /locus_tag="VFQ23_04385"
     CDS             2162..2638
                     /locus_tag="VFQ23_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905847.1"
                     /translation="MKSGFLFMVGFVAIMAMGANGRGGSNQLRSATENFQRPEMALAA
                     GYSPALNLDRCHSSLGGAGYQYINAALLDTTVDLLKPEALVYVPGPNGAYKLGAVAYM
                     VPVSAWHAIHPTEWPQAMGEQFHLNSSLGMYVLHVWNWENNPSGMFEDWNPKVPCV"
     gene            complement(2718..3185)
                     /locus_tag="VFQ23_04390"
     CDS             complement(2718..3185)
                     /locus_tag="VFQ23_04390"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9905848.1"
                     /translation="MNTIKTILYMGITHGLATFYLPYQLGVLGTRLFNPGIFRYFAFA
                     LWIIGTLVIIWCSIDIIRRGRGTPAHFDPPKELVITGLYRYVRNPVYLGALLVQLGFI
                     LWFGTGLLISYSVFFALAYHILIVFIEEPILKNEFGAAYEQYRQAVPRWIPRL"
     gene            complement(3289..3753)
                     /locus_tag="VFQ23_04395"
     CDS             complement(3289..3753)
                     /locus_tag="VFQ23_04395"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9905849.1"
                     /translation="MTALKSLLFLIIAPGMVAGYIPLVLLRSGPQIETGVFAYLAFPL
                     WLIGGVILLWSFWNFLIEGRGTPAPVDPPKELVAVGFYRYVRNPMYFGVLLILIGHFL
                     WFGYWWLLAYTILAFIVVHLFVTGYEEPTLKRKFGVAYEDYLKRVPRWIPRF"
     gene            complement(3750..4307)
                     /locus_tag="VFQ23_04400"
     CDS             complement(3750..4307)
                     /locus_tag="VFQ23_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9905850.1"
                     /translation="MMTKRQNPNAVANRQQILSELQMARATFHRLLDSLSNEDFYKPS
                     LNPGWTNGEILVHMTFGFIIVNVLLPMARLWGWLPKGSSKWFAWLLNAVTGPFNWINE
                     LGARGQGKIFTRTRIGTVYDRAYFSLLRKINSIKDEEWQRGMYYPTKWDPNFSEFMTL
                     EKLFHYPVNHFNFHLTQISHGRERM"
     gene            complement(4308..5792)
                     /locus_tag="VFQ23_04405"
     CDS             complement(4308..5792)
                     /locus_tag="VFQ23_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9905851.1"
                     /translation="MSGRVLLINPARHFIANQYGVGYLIPLGLVSIGGPLIDAGFTVK
                     LIDHDAYGWSLKKLINEVRKFQADYVLLGHSGSTASHNVAVKTFQEIKKALPQICTIY
                     GGVYPSYADKGILSECMDIDYIVRGEGEQTILELIQTLENKVSLEKIDGITWRNEAQI
                     VVNRSRMPIQNLDIYRPGWELVDWSLYKMFGFKRAAGLQFSRGCTLTCSYCGQWMFWK
                     KWRHRSPENIVEQLKILKNNYDVDYVWFADENFSADRETLIHILNLIIDAKLDLSLNI
                     NMTAADVVRDADIIPLYKAAGIDYIVMGVESLKDEVVSKIRKNNPFDISKEAVRLLRK
                     NNILSLTNIIYGLEDESWKTIREKFKNLLELDSDILNTCYLMPHFWTAQGRTAKPADV
                     IQSDLSKWTYRNQIITTPHLRPFELFMGVKLTEALFHLRPRALRRIFFGKDHRYIKIM
                     RSSMWAGIRVIIAEIIEFLFQTKFSPRGSLKYLPGPETHLPVLK"
     gene            complement(5903..6124)
                     /locus_tag="VFQ23_04410"
     CDS             complement(5903..6124)
                     /locus_tag="VFQ23_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905852.1"
                     /translation="MNSQQNKRMLLIIVGLVDAVISGAILLIYFGLLPIDVSEWGIPR
                     WVIGIVGGLWFLGAVGLVFYQLTRTDISE"
     gene            complement(6105..6398)
                     /locus_tag="VFQ23_04415"
     CDS             complement(6105..6398)
                     /locus_tag="VFQ23_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905853.1"
                     /translation="MKTILTPTILKIILTFVLFILTSLLWRNYVISTISDTFPWGFPL
                     QFYLAWGPCPPGETCYEFNGLWLLLDLVIWYVISAFIIYFASQRKGRNELSTK"
     gene            complement(6533..6760)
                     /locus_tag="VFQ23_04420"
     CDS             complement(6533..6760)
                     /locus_tag="VFQ23_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905854.1"
                     /translation="MDNNLSPEQRMRLIVMSVGAVNALLGAAITLIYLGFLPLNISYL
                     DIPNWVVGIIGASWFLTGVSVVAFAAPHVKE"
     gene            complement(6821..8545)
                     /gene="polX"
                     /locus_tag="VFQ23_04425"
     CDS             complement(6821..8545)
                     /gene="polX"
                     /locus_tag="VFQ23_04425"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HET9905855.1"
                     /translation="MMNNRQLADTFTLIADLLEIKGEIIYKTLAYRKASESLMASGRE
                     ASEFWKEGKLQDIPGVGKAIAEKIDELLSTGKLEFLEKIKKEIPPDLATWMKVPSLGP
                     KKIAMIHKALGITTLAELEAAAKEGKLRDLPSMGAKSETAILEGIASLGRRSDRIPLG
                     RAYPLALEIISQLKKVKGVVDAQPGGSLRRMRSTIGDLDILVSAKDSKPVMEAVVGLP
                     RVLRVLGKGDTKVSVEFNDGTRAQVWVHPPKEFGTAFLYATGSKDHNVRLREMALAKK
                     LSLSDHSFLKQNGEEIFCATEEEVYETLGLPWIPPELREDRGEIQAAKANNLPKLIEL
                     KDLKADLQTHSTWSDGKLSMLEMARAAAKRGIKVIAFTDHSVSLGVTQGLSMEDHKKQ
                     AAEIKKIQKQLGDSILVLHASEVEIKADGTLDYPDEFLASLDLVVASLHTSLRQPREK
                     VTQRVINAIRNPHVDIIGHPTGRLFPDREGADLDMEAVLKAAAETGVALEINAHPSRL
                     DLDDVHARRAKEMGIPISINTDAHSEEDMDLRFYGVAIARRAWLTKEDVINTWPTKKL
                     LSWLKKRG"
     gene            8680..9600
                     /locus_tag="VFQ23_04430"
     CDS             8680..9600
                     /locus_tag="VFQ23_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9905856.1"
                     /translation="MKPLSEFFKRHSLIIGILLMFAITWPIDLANAGLMPFEVPFVLY
                     LFLGWGFGIAAVLMTGLTLGKTGVISLLKRYLHWRVGWRWYLAAFLLSPGLIMGAVYL
                     NAAVTGVSPDFSTVMAYKIFGASAYLPLYVLPFFIIDFIANGEEIGWRGYVLPRLQVK
                     YNALASALILGLIWGAWHLPKFLTHWNTISFIWFMVHTVVASVLYTWLYNGTKGSLLL
                     VTLFHASSNTAGVFMPMANTVSSGNMGAYMIFVLLEAAAAILIILTTGLQRLSRKEQM
                     QVEEQSSGLSIQVFQSAAGLSGKPKHEGLR"
     gene            9711..10721
                     /locus_tag="VFQ23_04435"
     CDS             9711..10721
                     /locus_tag="VFQ23_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9905857.1"
                     /translation="MTTKTSHKILNWLKRAFVRVSILIFVLAMTGMIYQTAATESDQR
                     KYPPPGVLVNVNGYKMHIYCIGQGSPTIILDHVGDGSSVDWALIQPKLAEHTRVCAYD
                     RAGFGWSDSNPAPRTLEGQVNELHGLLQGANEHGPFILVGHSYGARVSRVYAAKYPDQ
                     VAGMVLMDAGILADDPRYPAEMQSDFETENNMLRAARWLSPFGLVRLTKPLMELPTFD
                     LPEEARTASASFSFSTRYFQSLIAQSHSMPTVLREEREVTSLGDIPLLVLVSTEPDDA
                     THKVWKQANIEMAALSTNGSYRIVDGATHMSLAYRKSDAQVCADGILEVLQSVRMVQA
                     LQ"
     gene            10742..11887
                     /locus_tag="VFQ23_04440"
     CDS             10742..11887
                     /locus_tag="VFQ23_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9905858.1"
                     /translation="MQSNVVLRLRDPFLLDLSTYLSVAAMALLGVSGLPNLSLQLAAL
                     ALCLTFALLYRFVFRTNYYERNPVLYFGAQAIVMVMLLLSSEASDFFNFLFYLLTIQT
                     AVVLNIRRATMWTAIYFGISAFAVFIARGTDGLFAILFYLVVFVICAVMGHSIQQADL
                     AREHNEQLLNELQATQQKLRDLAVVEERNRLARDLHDSVKQQVFAMSMQLGAARTALS
                     ETDQAYSSVIEAERLAQQAGAELSTLISALRPPSLENKTLTTAVREHVNEWSRQNKIS
                     TDLDIDSNILVNPHSEQVLFRVLQEALANVARHSEANEVRVSLNAEKDDVTLLIEDNG
                     IGFDTKQITKGVGLDSMKERLTAVNGTLEVTSIKPQGTRLTAKVRRS"
     gene            11888..12541
                     /locus_tag="VFQ23_04445"
     CDS             11888..12541
                     /locus_tag="VFQ23_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9905859.1"
                     /translation="MSESISVLLVDDHAMVRQGVRAFLITQPDISVVGEAGSGEEAVK
                     LSAQYIPDVILMDLIMPDMDGVEATRRVKHVSPRSQVVVLTSYHEDEHIFPALKAGAL
                     SYILKDVSAEELASAVRKAALGEAVLHPRVAARVIKELQGRRAEAINPFTELSERELE
                     VLKLIADGMSNAEMAEKLVLSEKTIKGHVSNILSKLHLADRTQAAVYAWREGVVRKE"
     gene            12571..13104
                     /locus_tag="VFQ23_04450"
     CDS             12571..13104
                     /locus_tag="VFQ23_04450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9905860.1"
                     /translation="MDHKDHVNLLRPANLEQGGIWADFGAGSGAFTLALREVVGPHTE
                     IYAVDKDKRGFNELEKSHREKFTTSQNLHPVQADFTRSLSLPPLDGIVMANSLHYYKD
                     KEKVLRHVRSFLKMNGALLVVEYNVDSGNMWVPHPLSFKTYQSLTPRAGFSEPRLLGT
                     APSRFLKEFYSAVAYRT"
     gene            complement(13396..15039)
                     /locus_tag="VFQ23_04455"
     CDS             complement(13396..15039)
                     /locus_tag="VFQ23_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9905861.1"
                     /translation="MRAGKQSYISFLWVVIFSACTAGTPATFVTPTNIVSETSTPTAT
                     SGTIKGQVIQSETNSPAAGVFVQAKNESGVIVTVISDSAGAFNITDLAFGAYTVSARG
                     TGFQNSDTQKLTVSGSESNQINISLSPVADIKSQLSGAAWLGLLPDDAETRQFILDCQ
                     GCHQLGWVRQQRGGWPSEAEWKTSINKMLGYYGPDSGFPIIGPVDADHTAAWLAQYLT
                     NDAPTHPSSLPISGEAANVRITEYDFPGTGPHDLVLDQNGQVIVTGMFSNDMWSLDPA
                     SGEFTRYDLPNDANPRAVEVDSNGVWWIVFGNPFQVARFDPAQQELTTTSVGMYAHSI
                     ALDGSGNAYINGHFKANPGKVVMLDSQGERVQTFDIPENNTSNLTGLPISYDLRVDAN
                     GIVWGSDLNWNRIYRLDPQTGDVKQYEFPNPVSGPRRFDIDADGNLWIPEFSGSRLAM
                     FNPVTETFTEWDTPTKNAEPYVVKVDNQNNQIWIGYAAGDRVARFDPATGTFTEYPLP
                     TRFALIRYMAVDQTSGDVWFSYHHIPTAQDMVARLETTS"
     gene            complement(15100..>15621)
                     /locus_tag="VFQ23_04460"
     CDS             complement(15100..>15621)
                     /locus_tag="VFQ23_04460"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905862.1"
                     /translation="AMTVYLPLISDQIATGSSQVFGVLLGALALGEVLSAWLAGSLIL
                     NMTLGKRIALAQILSGLSLALLLIGPSAFWSVATGLFLLGFFSAPLTIWAQTLRMQVI
                     PPEMRGRTFALLRTLMQGATPLGGALAGFLLPVWGMQWIVGLSSLVIGTPGLAGLQVE
                     ELTQAGHNTSLAG"
BASE COUNT         3856 a         3823 c         4006 g         3936 t
ORIGIN      
        1 tacatcgcaa gaccttgaac gccctccagg aatcgtctgt cacgcgggaa agtatgacgc
       61 ggcttgccca tcatgctgaa gcgatccatg atcctgatgc cgtcctcgaa tttgctccgc
      121 tcgcagcctg gtgggcatct actaccagtt ctcatcgtga ggcggttgca ctgtatgaac
      181 tagccttacg ctttgcgaaa tcccttccgc ctgccaacca tgcacagatg ctggaagcgt
      241 atatggttga gctggagttt atgaacagat tctctgatga aatcgaggct tgccgaagcg
      301 cgatcgcgct ttggcattcg atcggtaacc gtttacggga aggcaaaaac ctggcaacgt
      361 tggcgggact ttgtatgaat gtcggttgga ggtcggaagc ggaaaaaagc attgaacgcg
      421 ccatcactat ccttgaagac ctttccccaa gcgccgaatt ggcacaggcc tatacagaac
      481 agtgttatat caggatgatg tatcgggatt gtgaggcggc tcttccctgg ggcgagaaag
      541 ccattaagtt ggcagagcga ttcgaagatt tcgatgccct ggccagagca tacgattata
      601 tgggttgcgc ggctttaatc attgattatg atcgtggcct gaaattgctt gaacggagtc
      661 tcgttattgc acgcgaaaaa aaacttccgt tcgcggtagg cggcacgctg ggaaatttag
      721 gcggaatgtt gctggaggtg cgcgaagtca ccctcgcaaa tcaatatctg gcagaaagca
      781 taaactatgc taggcagcag gataatgatt accacttaat ggagtcccag gcctggcagg
      841 cattggcaaa tatgtatcag gggaagtgga tagaggctga tacggtcgct cgaaagatct
      901 tagaacattc gtcgaatcta ccgaatgtga cacgcgccag cgtattattt actcttggac
      961 gcattcgtat tcgccagggc gggaacaacg tagatcagtt actggacgaa gccttctctg
     1021 tctccatcca ggcggatgct ccacgtttcg attcgccgcg gctattacaa gcagagtttt
     1081 actggttgat cggcaatcaa ccccgggttt tagaagaaac tcatactgct tatgaacttg
     1141 cccttaggaa taagcatccg tggattgcgg gcgagcttgc cttctggcgc tggcgttcag
     1201 gtgacaaaaa ttctccgcca gcatggattg caaagccttt tgcgcttcaa attgcgggcg
     1261 attggcacgc tgccgcaaaa gagtgggagg aacgcggctg cccctatgaa caggcgatgg
     1321 ccctggtgga tggcgatgac gccgcccaac tcgccgcatt ggacatcttt gagcggcttg
     1381 gagcgctccc tgctcttgat attctcaagc gaaaaatgcg tgctgacgga acccgcatcc
     1441 cgcgcagacc gcgtcctgct acgcgagaaa acccgtttaa tctcacggcg cgtgaaatgg
     1501 aagcgctggt ttgcctggta gacggtttga gtaatcatgc cattgctaaa aaactgagcc
     1561 tatccactcg cacggccgag catcacatcg cgtcgatttt gcaaaaaatg ggggttgggt
     1621 cgcgcagtga ggctgtggca ctggctttga aagagaattt acttcgtact gaataacgta
     1681 aggcgctgtc cgcagcttcg attccccaaa gtacaactca aaataggtag tccgactcac
     1741 caatataggt agagtcaccg atgtgtttta gcctgccccg tcgtaagata ttggcaggct
     1801 taaatgaggt ttgatctgga aggagaaagg catggacaaa tactattttt atcaccaact
     1861 tgcacaagaa catcagtacg aagtttctaa ggagttggcg aaccgtcatt tgctcaggaa
     1921 tggcaaggag tatccattca cagcgaaaca agcccggcgg ctggtatgga gagcggcatt
     1981 tgctgtggtg acctttatat tactcgcatt ctatatgatc ggttaatgaa attacgatcc
     2041 tcgttcgtga atctgtgtgc ttgatgagac tctttcacgt tctcctccaa acaagcacac
     2101 gttacacccg catcgaggca atccctgatg cgggttcgca ttcaactgaa gggagatggc
     2161 aatgaaaagc ggttttttgt tcatggtagg gttcgttgca attatggcaa tgggtgcgaa
     2221 tgggcgaggt ggatcaaacc aactccgctc tgcgactgaa aattttcagc gccctgaaat
     2281 ggcgctggcc gcaggttaca gccccgcatt aaatctcgat cgttgccatt ccagccttgg
     2341 cggggcaggt tatcaataca tcaacgctgc tttgcttgac acgactgtgg atctcctaaa
     2401 acctgaagca ctcgtttatg tcccgggtcc aaatggagcc tataagcttg gcgcggtggc
     2461 atatatggtt ccggtgtcag cgtggcatgc catccacccg acagaatggc cgcaggcaat
     2521 gggtgaacaa ttccatctaa actcgagcct gggtatgtat gttcttcatg tttggaactg
     2581 ggagaataat ccctcaggaa tgtttgagga ctggaatcca aaggttccct gtgtgtaata
     2641 tgtagtacag accccgaagt attagaatac ttcggggtct tcgtcgctac ttggaatcat
     2701 ctatcggggg ttctaattta cagccgtggg atccaacgcg gcacagcttg gcgatactgt
     2761 tcgtatgccg cgccaaattc atttttcagg atgggttctt caataaacac gatcagaatg
     2821 tgataggcga gagcaaaaaa tacagaataa ctgatcaaaa gtccggtgcc aaaccaaagg
     2881 ataaagccca actggaccag cagtgcaccg agataaacgg ggttccggac ataacgatat
     2941 aacccggtga tgaccagttc cttcggcgga tcaaaatgcg cgggagtccc gcgtccacgg
     3001 cggataatat ctatcgagca ccagatgatg acgagtgttc caatgatcca cagtgcaaaa
     3061 gcaaaataac gaaagatgcc cgggttaaat aaacgcgtcc ctaacacgcc aagctgatag
     3121 gggagataaa aagtagcgag tccgtgtgtg attcccatgt agaggatggt tttgatagtg
     3181 ttcatgataa atcagaccct agtggtttcc atgactgact tatactttta attgtaagac
     3241 tttcatgcct tacataagga atattcatgc gaaaaaccat tcgggtctct aaaatcgtgg
     3301 aatccagcgc gggactctct tgagatagtc ttcgtacgcc accccaaatt ttcttttcaa
     3361 ggttggttct tcatatccgg tgacaaacaa atgaacgaca atgaaagcca gaatggtata
     3421 agccaataac caccaatatc caaaccacag aaaatgccca atcagaatga gaagaacacc
     3481 gaaatacatc gggttacgca cataacgata aaacccgacc gcgaccaatt ccttgggtgg
     3541 gtcaacggga gccggggtgc cgcgtccttc aatcaggaaa ttccaaaatg accacaacag
     3601 gatgactcca ccgatcagcc agagcggaaa cgcgagatac gcaaagactc ccgtctcgat
     3661 ctgtggcccg ctgcgcaaaa ggaccagagg gatatagcct gcaaccatcc ccggtgcaat
     3721 gatcaaaaac agcagagatt ttaaggctgt cacatccgct cccttccatg cgagatttgg
     3781 gtcagatgaa agttgaaatg gttgacaggg taatgaaaca atttttcgag agtcataaat
     3841 tcactgaaat tcggatccca ttttgtgggg taatacatgc cgcgctgcca ttcctcatcc
     3901 ttgatcgaat taatttttct cagaagtgaa aaataggccc ggtcataaac tgttccgatt
     3961 cgagttcgag tgaaaatttt tccctgcccg cgggcgccaa gttcattaat ccagttaaag
     4021 ggacctgtga ctgcatttaa taaccaggca aaccatttgg atgagccttt cggtaaccag
     4081 ccccacaagc gcgccatcgg taaaagcaca ttgacaatga taaatccgaa cgtcatgtga
     4141 accaggattt cgccgtttgt ccagccgggg ttgaggctgg gcttataaaa atcttcgttc
     4201 gatagtgaat caagtaatcg gtgaaacgta gcgcgcgcca tctgaagttc actgaggatt
     4261 tgttgtcgat ttgcgactgc gtttggattc tgacgtttgg tcatcattta tttcaatacc
     4321 ggcaaatgtg tttcaggtcc tggtaagtat ttcagacttc cgcgaggaga gaatttggtc
     4381 tggaaaagga attcaataat ctctgcaatg ataacgcgta tccccgccca catggaggaa
     4441 cgcatgatct tgatatagcg atggtctttt ccgaaaaaaa tacgccttaa tgcccttgga
     4501 cgcaaatgaa acagggcttc tgtcaatttg acgcccatga ataactcgaa tggccgaaga
     4561 tgtggcgtgg tgatgatttg atttcgatac gtccacttac tcaaatcgct ttggatcacg
     4621 tctgctggct tggcggttcg accctgggct gtccaaaagt gtggcatgag atagcaggta
     4681 ttgaggatat ctgaatcaag ctcgagcagg tttttgaact tctctcggat agtcttccaa
     4741 ctttcatcct ccagtccata aataatgttt gtcaggctga ggatgttatt ctttcgtaac
     4801 aaacgaacgg cttcttttga gatgtcaaac gggttgttct tgcggatttt ggacaccacc
     4861 tcatctttga gcgattccac ccccatcaca atatagtcga tgcctgcggc tttatagagc
     4921 ggaatgatat cggcgtcgcg cacaacatcc gcggcggtca tattgatgtt gaggctgaga
     4981 tccagtttgg catcgatgat caggttcagg atgtgtatga gcgtctctcg atctgctgag
     5041 aagttttcgt ccgcaaacca gacgtaatcc acatcatagt tattctttag aattttcaac
     5101 tgctccacga tattttcggg actgcgatgc cgccactttt tccagaacat ccattgtccg
     5161 cagtaactgc aagtgagcgt acagccgcgg ctgaattgca agcctgccgc acgtttgaat
     5221 ccaaacattt tgtaaagtga ccaatccacc agttcccagc ccgggcgata gatatccaga
     5281 ttttgaatcg gcatgcgcga acggttcacg acgatttgtg cttcgtttcg ccaggtaata
     5341 ccatcgatct tttcaaggct cactttattt tccaatgttt gaatgagttc gagaatcgtt
     5401 tgctcccctt cgccgcggac gatgtagtct atgtccatac attcgctcag aatgccttta
     5461 tctgcgtacg atgggtagac accaccgtag attgtgcata tttgtggaag cgcctttttg
     5521 atttcctgga aggttttgac ggctacgtta tgtgaggcgg ttgacccaga gtgtcccaat
     5581 aacacataat ccgcctgaaa ttttcggacc tcattaatta gctttttgag tgaccagccg
     5641 taggcatcgt ggtcaattaa tttcactgta aagcccgcat cgatcaaggg tccaccgata
     5701 ctgaccaggc cgaggggaat gaggtagccg actccgtact gattggcaat aaagtgacgc
     5761 gccgggttga tcaacaggac tcggccagac ataacgcctc caaatcggac tatttaggta
     5821 caatttgtga ataatatcac tatcggaggt tttgtcaaga gcttcctatc gtcgcaaaga
     5881 cctgacaggt ctttcgaaat tcctattctg agatatcggt ccttgtcaac tgataaaaaa
     5941 cgaggcccac cgctcccaga aaccagagcc caccaacaat accgatcacc cagcgcggga
     6001 ttccccactc ggagacgtcg atgggaagca gtccgaaata aatcagtaaa atagcgccgc
     6061 ttatgacggc atctacaaga ccaacgatga tcaaaagcat gcgtttattt tgttgagagt
     6121 tcattacgcc ctttcctttg cgatgcaaaa taaatgatga acgcgctgat tacataccag
     6181 atcacaagat caagcaaaag ccatagtccg ttgaattcat aacaggtctc gcccggtggg
     6241 caaggtcccc aggcaagata gaattgaagc ggaaaacccc atgggaaggt atctgaaatc
     6301 gtagagatca catagttgcg ccagagcagg cttgtgagaa taaacaaaac aaaggtcaga
     6361 atgatcttca gaatagtagg tgtgaggatg gttttcatct tctttcataa ttcatattgt
     6421 tggtcgagta gccgaaggca tatcgagatc aataaataat cagaagaact catgtttgtg
     6481 gtggtttcga tacgccctgc catgaacgcg caggactact caaccaccaa agttattctt
     6541 tgacatgcgg cgcagcaaaa gcaactacac ttacacccgt aagaaaccaa gacgccccga
     6601 tgatgccaac aacccagttt gggatatcca ggtaagagat attaagaggt aagaacccta
     6661 agtaaatcag tgtaattgcc gcgcccagca gggcattgac tgcaccaact gacattacga
     6721 tcaaacgcat tctttgttcg ggggatagat tattgtccat attatgctcc tcttaacaaa
     6781 cgtgacagtc acctcgcgaa gcgggtgact gtcacgtatc tcaaccacgt ttcttcagcc
     6841 aactcaacag cttcttggtg ggccaagtat tgatcacatc ctcttttgtc agccaggcgc
     6901 ggcgggcgat ggcgacccca tagaagcgca ggtccatatc ctcttcggaa tgcgcgtcgg
     6961 tgttgatgct gatagggata cccatttctt ttgcgcggcg ggcatgtacg tcgtccaggt
     7021 cgaggcggga ggggtgtgca ttgatctcca acgcgacgcc ggtttccgct gcggctttga
     7081 ggacggcttc catgtcgagg tccgcgcctt cgcgatctgg gaataaacgt cccgtggggt
     7141 gaccgatgat atccacatgt gggttgcgaa tggcgttgat cacgcgctgt gtcactttct
     7201 cgcgcggctg tcgcagagac gtatgaagcg aggcaacgac taaatccagc gaagcgagaa
     7261 actcatccgg gtaatcgagc gtgccatcgg ctttgatctc aacctcgcta gcgtgtaaca
     7321 ccagaatgct atcgccaagc tgcttttgga tctttttgat ttcagccgcc tgtttcttgt
     7381 gatcttccat gctcaagccc tgtgtgacgc cgaggcttac agaatgatct gtaaatgcaa
     7441 tcactttgat gccgcgtttt gccgcagcac gtgccatctc cagcatggac agtttgccgt
     7501 cgctccatgt ggagtgggtt tgcaggtcgg ctttgagatc ctttaattcg atcaactttg
     7561 gaagattgtt tgctttcgcc gcctgaatct caccacggtc ttcgcgcaat tcaggaggaa
     7621 tccatggcaa gccaagtgtt tcataaactt cctcttctgt cgcgcaaaat atttcctctc
     7681 cattttgttt gagaaaggaa tgatcggata acgacagctt ctttgccagc gccatttcac
     7741 gcaaccggac attgtgatct tttgagccgg tagcataaag aaacgcggtt ccaaattctt
     7801 ttggcggatg tacccatacc tgggcacggg tgccatcatt aaactcgacg ctcacctttg
     7861 tatcgccttt gccgagcacg cgcaggacgc gtggcaaacc gacaacagcc tccatcactg
     7921 gcttcgaatc ttttgccgag acgaggatat ccaggtcacc gattgtggag cgcatccgcc
     7981 tgaggcttcc accgggctgt gcatcaacga cacccttcac ctttttgagc tgactaataa
     8041 tttccagagc caggggatag gcacgcccca acggaatccg gtctgacctt cgtcccagcg
     8101 aggcaatccc ttccagaatg gcggtctctg attttgcgcc catgctgggg aggtcgcgca
     8161 gctttccttc tttcgcggca gcttccagct ccgccagagt tgtaattccc aatgccttgt
     8221 gaatcatcgc aatctttttg gggcccaggc ttggcacttt catccatgtc gccaggtcag
     8281 gggggatttc cttcttgatc ttctcaagga attcaagttt tccggtggag agcaattcat
     8341 cgattttttc tgcgatggct ttgcccacgc caggaatgtc ctgcagtttt ccttctttcc
     8401 aaaattcaga tgcttcgcgt ccactggcca tgagactttc ggatgctttc cggtacgcca
     8461 atgttttgta aatgatctcg cctttgatct ccagtagatc ggcgatgagg gtgaatgtat
     8521 ctgctagttg tcggttattc atcatagtgc tcatcctttc gctacgtgga ttatacgctc
     8581 aaacgatacc gcctcaggtc gcatcggaat acggtctcca gactcgtcac ttgtggaggg
     8641 tataggcgta tcctatgagc aatcaaaaag gagtttgcca tgaaaccatt gtcagaattt
     8701 ttcaaacgac attcgctcat catcgggatt ttgctgatgt ttgctatcac ctggcccatt
     8761 gatctggcga atgcagggct aatgcccttc gaggttcctt tcgtcctata cctcttcctg
     8821 ggctggggct ttggaattgc ggcagtcctg atgacgggac tgactctcgg aaaaacaggt
     8881 gtgatctcac tcctgaaacg atatttacat tggcgtgttg gctggaggtg gtatcttgcc
     8941 gcgtttcttc tctctcccgg tttgataatg ggagctgtgt atctcaatgc cgcggtcact
     9001 ggcgtctcgc ccgatttcag tacagttatg gcatacaaga tttttggagc gtcagcttac
     9061 ctgcctctgt atgtcctgcc ctttttcatc attgatttca ttgccaatgg agaggaaatc
     9121 ggctggcgcg gctatgttct gccgcgtctt caggtgaagt ataacgcgct agcctccgca
     9181 ctgatcctgg gcttgatctg gggagcctgg catttgccga aattcctgac gcactggaat
     9241 acgatttcct ttatctggtt tatggtgcac actgtggtgg cttcggtgtt gtatacctgg
     9301 ctgtataacg gcacaaaagg gagtctgctt ttggttactt tgttccatgc cagttcgaat
     9361 accgcaggcg tcttcatgcc aatggcaaat actgtgtcca gcggaaatat gggcgcttac
     9421 atgatctttg ttctgctcga agcggcagca gcaattctga tcatccttac aactggactc
     9481 caacggctct cgcgcaaaga acagatgcag gtggaggaac agtcatccgg cttatcaatt
     9541 caggttttcc aatcggctgc gggtctatcg ggaaagccta aacacgaagg cttgcgctga
     9601 gtctgtcgaa gtgacacaaa gagcacaaag gtttaaaaaa acgaattttc ccctttgtgc
     9661 tctttgtggt tgaaaagagt tatccggtta gaaacgaaaa ggcaatcata atgacaacaa
     9721 aaacatctca caaaatttta aattggctca aacgcgcgtt cgtccgtgta tctatcctca
     9781 ttttcgtgct ggcgatgacg ggaatgattt atcaaactgc tgcaaccgaa tcggaccagc
     9841 gcaaatatcc gccgcctggg gtcttggtca atgtcaatgg atacaagatg cacatctatt
     9901 gcatcggaca aggaagccca acgatcattc tcgatcatgt cggtgacgga tcatccgtgg
     9961 actgggcctt gatccagcca aaattggcgg agcatacgcg agtctgcgcg tacgaccgcg
    10021 cgggtttcgg atggagcgat tccaatcccg cgccgcgcac gctggaggga caagtcaatg
    10081 aattacatgg tcttctgcag ggtgcgaatg aacacggacc ctttattttg gttgggcatt
    10141 cctacggcgc acgcgtcagt cgcgtttacg ccgcgaaata tccagaccag gtggcaggca
    10201 tggtcctaat ggacgcgggc atccttgctg atgatccgcg ctacccggca gagatgcaat
    10261 cggattttga gacggagaat aacatgctcc gcgctgcgcg ctggctgtcg ccgtttggct
    10321 tggtgcgctt aacaaaacca ctgatggaat tgccaacttt tgacttgcct gaagaagccc
    10381 gcaccgcaag cgcttccttt tccttctcga cccgttactt tcagagcctc atcgcccaga
    10441 gccattcaat gccgactgtt ctccgggaag agcgtgaggt gacctcgctg ggagatattc
    10501 cccttttggt tttggtttcc accgagcctg atgatgccac tcacaaagtt tggaaacagg
    10561 caaacattga aatggcggct ctctccacga atggaagtta tcgcattgtt gatggcgcga
    10621 cgcacatgtc attggcatat cgcaaaagcg acgcgcaggt ctgcgcggat ggaatcttgg
    10681 aagtgctgca atcagttcgc atggtgcagg ctttgcaata atcgcgagta caatgaaaaa
    10741 gatgcaaagc aacgttgtac tgcgtcttcg cgatcctttt ctattggatc tatcgacata
    10801 tctcagcgta gccgccatgg ctttgctggg ggtgagcggt cttccaaatt tgtcattaca
    10861 gttagctgca ttggcattgt gtttgacctt tgccttacta tatcgctttg tattccgaac
    10921 aaattattat gaacggaatc cggttctcta ttttggagcg caggcaattg taatggttat
    10981 gttgttgttg agctcggaag ccagcgattt cttcaacttc ttgttttatc ttctgacaat
    11041 ccagacggca gttgtgctga acattcgccg ggcgacgatg tggacggcca tctactttgg
    11101 tatttctgca tttgctgtgt ttatcgcccg aggcactgac gggttgtttg cgatcctctt
    11161 ttacctcgta gtttttgtca tttgtgctgt catgggtcat tccattcagc aggcggatct
    11221 ggcgcgcgaa cataacgaac aactactgaa cgaattacag gcaacgcagc aaaaactccg
    11281 tgatctggca gtggtggagg aacggaatcg cctggcgcgt gacttgcatg actctgtaaa
    11341 acagcaggtc ttcgcgatgt cgatgcaact gggcgcggca cgcacagcgt tatccgaaac
    11401 tgatcaggcc tattcgtccg ttatagaagc cgaacgtctg gcacaacagg ctggggcaga
    11461 gttgtccaca ttgatctccg cgttgcgtcc gcccagcctg gagaataaga cactgactac
    11521 cgcagtcagg gagcatgtca acgaatggtc gcgccagaac aaaatatcaa cggacctgga
    11581 tatcgactcg aatatcctag tgaacccgca tagtgaacag gtcttatttc gagtacttca
    11641 agaagccctg gcaaacgtgg cgcgccatag tgaagctaat gaagtgcggg tcagtttgaa
    11701 cgcagaaaaa gacgatgtta ccttacttat cgaagataat ggcattggtt tcgacacaaa
    11761 gcagatcacg aagggagtcg gtctggactc aatgaaagaa cgacttactg ctgtaaatgg
    11821 aactctggaa gtcacaagca tcaagcctca agggacacgc cttactgcaa aggtcaggag
    11881 gtcataaatg tcagaatcga tttccgtttt gctggtggat gatcacgcga tggtgcgaca
    11941 gggtgtgcgc gcttttttga tcacacagcc agatatctct gttgttggtg aggcggggtc
    12001 aggcgaggaa gctgtgaaac tatccgcgca atatatcccg gatgtcattc tgatggacct
    12061 catcatgccc gatatggatg gcgttgaagc cacgcggcgc gtcaagcacg ttagtccgcg
    12121 ttcacaggtg gtggtgctga cttcgtatca tgaagacgag catatcttcc ctgcgttgaa
    12181 ggcaggggcg ttatcttaca tcctcaagga tgtgagtgcg gaagaattgg catctgcagt
    12241 gcgaaaggcg gctcttggtg aagccgtcct gcatccgcgc gtggcggcgc gcgtcatcaa
    12301 ggaattacag ggcaggcgcg cggaagcgat caacccattc accgagctaa gtgagcgcga
    12361 acttgaagtg ttaaagttga ttgccgatgg catgtcgaat gcagagatgg cggagaaatt
    12421 ggtactgagc gagaaaacga tcaaaggcca tgtgagcaac atcctgagca aactgcatct
    12481 ggcagatcgg actcaggccg cggtatatgc gtggcgcgag ggagtagtgc gcaaggagtg
    12541 aattcatttg ggttgtgata ccattctcat atggatcaca aggaccacgt caatctatta
    12601 agacctgcca atttggagca aggtgggata tgggcagatt tcggtgcagg ctcaggggcg
    12661 ttcacgctgg cgttgcgcga agtagttggc ccacacaccg agatctatgc agtggacaag
    12721 gacaaaaggg gattcaatga gttggaaaaa tcacatcgcg agaaattcac aacgtcgcag
    12781 aatctgcacc cggttcaagc tgacttcacc cgctcacttt cattgcctcc actggatgga
    12841 atcgtgatgg cgaattcgct gcattattac aaagataaag aaaaggtatt gcgacatgtg
    12901 cgctcgttcc tgaaaatgaa tggcgcgtta ttggttgtcg aatacaatgt cgattcgggg
    12961 aatatgtggg tgccacaccc gttgtcattt aagacttatc aaagtttgac gccacgcgca
    13021 ggtttcagcg aaccgcgctt gttgggaacc gcgccttcca ggttcttgaa ggaattctat
    13081 tcagcagtgg cttatcgaac ttaattaccc gccttatctg gattttctgc cacagagtca
    13141 gtgagaatgc tgagaaaagc tcattatttt ctctgagact ctgcgtctct gcggctcggc
    13201 aagtattgtt agctcatgat ctgctacgcc tgcaaatctt caggacggat tggcatgcgg
    13261 cggatacgga tgcctgtcgc gttgtagatc gcgttgacca ccgctggcac aatcgtaggc
    13321 acaaccgttt caccaagttc ctgcgaaaaa tacggcctgt cttgaagccg tgcaactgct
    13381 tcaagagatt attacctaac tggtggtttc cagacgtgcg accatatctt gtgctgtcgg
    13441 gatgtgatgg taactgaacc agacgtctcc agaggtttgg tcaactgcca tataacggat
    13501 cagggcgaac cttgtgggca ggggatactc agtgaaggtc cctgtcgctg ggtcgaagcg
    13561 cgcaacccta tcacctgcgg cgtatccgat ccaaatctgg ttgttctggt tatccacttt
    13621 taccacatac ggttccgcat ttttggtcgg cgtatcccat tcggtaaagg tctcagttac
    13681 cgggttgaac atcgccagtc gactgccgga gaactcgggg atccataaat ttccgtccgc
    13741 atcaatatca aatcggcgcg gtccactgac cgggtttgga aattcatatt gtttgacatc
    13801 gccagtttgt gggtcgagcc ggtagatgcg attccaattg aggtcagatc cccagacgat
    13861 cccatttgca tccacacgca gatcataaga aatggggaga cctgtcaggt ttgacgtgtt
    13921 gttttcagga atatcgaatg tctggacccg ctcaccctgc gaatcgagca tgaccacttt
    13981 gccggggttc gctttgaaat gtccattgat gtatgcattc ccacttccat ccaaagcaat
    14041 gctgtgcgca tacatgccca cggaggtcgt tgttaattcc tgttgagcag ggtcaaagcg
    14101 cgccacttga aacgggttac caaatacgat ccaccatact ccatttgaat cgacctccac
    14161 ggcgcgcggg tttgcatcgt ttggcagatc gtagcgtgtg aattctcccg atgccgggtc
    14221 taacgaccac atgtcattgc tgaacatgcc agtgacgatg acctgaccat tttgatccag
    14281 caccagatca tgaggtccgg ttccggggaa atcatattca gtgatgcgca cgttcgcagc
    14341 ctcgccgcta atgggaaggg acgatggatg cgttggcgca tcgttcgtca gatattgagc
    14401 aagccacgcc gcggtgtgat ctgcatccac cggaccgata attgggaagc cactatccgg
    14461 cccgtaatag cccagcatct tgttaatgga cgtcttccat tcggcttcag agggccaacc
    14521 accgcgttgc tgcctcaccc agccgagttg atgacaaccc tgacaatcca atatgaattg
    14581 gcgagtctcg gcatcatccg gcaaaagccc aagccacgcc gcgccgctga gctgtgattt
    14641 aatatcagcc acaggactca acgaaatgtt gatctggttc gattcagagc cgctcacggt
    14701 gagtttctgg gtatccgaat tttgaaatcc cgtgccgcgt gctgaaacgg tatacgcacc
    14761 aaaagccagg tctgtgatgt tgaatgcgcc tgcagagtcg gagataacgg ttacgatcac
    14821 gccggattca ttctttgcct gaacaaagac acctgcagca ggagaattcg tttcgctttg
    14881 gatgacctga cctttgatcg tcccactcgt tgcggttggt gttgatgttt cggatactat
    14941 atttgttggg gtaacgaagg ttgcaggggt gcctgctgtg caggcgctaa aaatcaccac
    15001 ccataggaac gaaatgtaag attgttttcc agcgcgcatg gaaatctcct agtaaagatt
    15061 ttcgtgcgct gaaattgtag gggagcggtt ttggttctgt caacctgcca gcgaggtgtt
    15121 atggcccgct tgcgttaatt cttcgacttg aagcccagcc agccccggtg tgccgatcac
    15181 gagcgatgac aagccaacaa tccattgcat tccccagaca ggcagaagga aaccggccag
    15241 tgcgccgccc aggggagtcg ctccctgcat cagggtgcgc aggagggcaa aggttcgtcc
    15301 acgcatttcc ggcgggatga cctgcatgcg caaagtctgc gcccagatcg tgagcggcgc
    15361 actaaaaaat cccagcagga acagaccggt cgccacggac cagaaagccg aaggtccgat
    15421 taagagcaat gcaagactca accccgagag aatctgtgcg agcgcgatac gtttgccaag
    15481 agtcatattc aaaatgagac ttcccgccag ccacgcgctg aggacttctc caagcgcaag
    15541 tgccccaagc aacaccccga agacctgcga actgccggtt gcaatttgat cagagatgag
    15601 aggtagatag acagtcatcg c
//
LOCUS       DASTJP010000147        10944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1084830, whole genome shotgun sequence.
ACCESSION   DASTJP010000147 DASTJP010000000
VERSION     DASTJP010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084830"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFQ23_04465"
     CDS             complement(<1..311)
                     /locus_tag="VFQ23_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905863.1"
                     /translation="MLDFLLIALVGWPAIVGTIILALIGLIRRDYRFLVAAAILAFPF
                     AWYLSGFPIIRSPAFLLPLLLFTSGFAMNRGREMIAWLLTIPYFLSIILLLFAVLAGN
                     P"
     gene            complement(327..659)
                     /locus_tag="VFQ23_04470"
     CDS             complement(327..659)
                     /locus_tag="VFQ23_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905864.1"
                     /translation="MNTLGTVICFGIIMGFVAVIAVIGTRSRLRFARYLQSMNREEKI
                     KFTEKNFRFITIFGVGAAIGILGILSSLVFNQEYLANFFFLGLFVYFLVMAMLAELFI
                     YRVPKNRK"
     gene            complement(688..2091)
                     /locus_tag="VFQ23_04475"
     CDS             complement(688..2091)
                     /locus_tag="VFQ23_04475"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HET9905865.1"
                     /translation="MSTLLIKNAHIVTMDDHQTEIAEGGLFIRRGLIQEVGRMSDLPQ
                     AADEVLDLKDHIVLPGLVNTHHHFYQTLTRAVPAAQDANLFNWLTTLYPIWARIRPED
                     IFTSTQTALAELALSGCTTASDHLYLFPNGSKLDDEIAAAQEIGLRIQASRGSMSLGA
                     SQGGLPPDSVVDDEDSILKDSQRLIEAYHDAKPGAMVQIVLAPCSPFSVTTDLMKQSA
                     KLAREYGVHLHTHLAETEDEEQFCLQKFGHRPVGYMQEVDWVGDDVWFAHAVWVNDEE
                     IKVFAKHNCGVAHCPTSNMRLASGIAPLKEYRAAGVPVGLGVDGSASNDGSHLLAEVR
                     NALLLSRVKEGITGFSATPSPGGRGEGEGERKLMTAREALYLGTRGGAAVVGRNDIGS
                     LEAGKCADFFAINLNRIGYAGMHDPVAAVVFGQPVNADYTVVGGKFVVKEGQLVTVDE
                     RKLAEKHNKAAKRLLAS"
     gene            2230..2760
                     /locus_tag="VFQ23_04480"
     CDS             2230..2760
                     /locus_tag="VFQ23_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9905866.1"
                     /translation="MTIKISVYIATSLDGFIARRNGDIDWLTGGEGGEDYGYAEFMSD
                     IDHIVMGRNTYEKVLTFGGWPYAKKVIVLSSRNLSIPPELSDKVEALNLSPRELISEM
                     DTRIAKHIYLDGGVTIQRFLREGLVDELTITTIPILIGEGLSLFGELERDVKLELIKS
                     ESFMNGFVQNRYKVLR"
     gene            3105..3425
                     /locus_tag="VFQ23_04485"
     CDS             3105..3425
                     /locus_tag="VFQ23_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905867.1"
                     /translation="MTIHTSPSSVLTKVAKSLVAAKRYSTTEEALWDMALSTVRGKVA
                     YYRRRIRRLENKHGVDFDKFTARLKGKATPAQEDDWLMWKSARSMLRDWQATYKDLLR
                     EQPG"
     gene            3409..3798
                     /locus_tag="VFQ23_04490"
     CDS             3409..3798
                     /locus_tag="VFQ23_04490"
                     /inference="COORDINATES: protein motif:HMM:NF039803.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HET9905868.1"
                     /translation="MNNLVDDILELLQTHALVKATRVVYYDETPSGKLEVKIRSELPR
                     NHKLQIWIHLETDSLDYAYQVFTTIPLLRWDNSPHYGHLKSAPHHFHNENGRVYDSPL
                     TGNIKKDLKVVLQEIKDWMEVQQEEHK"
     gene            3908..4756
                     /locus_tag="VFQ23_04495"
     CDS             3908..4756
                     /locus_tag="VFQ23_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9905869.1"
                     /translation="MKPLYNQFVQKSPSQIIKTSRGAIEYALHNPNGGATVILCHSTG
                     GGYDQGLVLAQLLDGFQGIAVSRAGYLRTPVETGASPAELADAYEELLDSLEVEKAAI
                     LGLSGGGMSAAHFAFRHSDRCWGLVLADAITKAPPKSSARIVATANSLPDGIAWLLTR
                     LAIYIGLPLMVRDAETRSMMRVFFENNPISERRAGVRNDLMNVNAMNEFPWEEICVPT
                     LLIHGDKDNLIPVEYSREVARRISNAELVIVKGGGHECLVSHQREVAPMLNSFLKKYI
                     SSPPDR"
     gene            complement(4840..5349)
                     /locus_tag="VFQ23_04500"
     CDS             complement(4840..5349)
                     /locus_tag="VFQ23_04500"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET9905870.1"
                     /translation="MQFEIGDQVVHPQHGVGQVVKLEDKEFETGVTRTYYEISMKGGS
                     TIWAPVDLGTIGLRKPAGRSAIARCRKILVSRAAHLPEDSRSRQSDLLARLRKGTIST
                     FCEVVRDLYAYGEHKSLFGTIAGFFRVTKDVLCQEWAVVEGVTYAEAVQEVDALLEKG
                     REGMKVPKK"
     gene            complement(5359..5667)
                     /locus_tag="VFQ23_04505"
     CDS             complement(5359..5667)
                     /locus_tag="VFQ23_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET9905871.1"
                     /translation="MEAKLYVGNLSYDTTEQGLRELFMQAGNVVSVALIKEPGTNRSK
                     GFGFVEMASQSEVQKAISMFNGYTLNDRQLKVNVAKPREERGGGFRQQRGRNDNRRRY
                     "
     gene            5921..6742
                     /locus_tag="VFQ23_04510"
     CDS             5921..6742
                     /locus_tag="VFQ23_04510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9905872.1"
                     /translation="MNHIQADMSDEALVTAIRNNMCDFFRHVSRSDLQEHFESQQFTR
                     WHSPLPHPWFNGVLSSRAYEEGDDAFIEATIQYFREKGILSFTWWMEPHVSPPAWEQI
                     QSRHGFRYSNDTPGMAIDLNDLNDSPRLLEGFEIRVVNDEETLHAWVKTFIHGYGLPS
                     HWESLTFDLWVQLGLDLPIRNYLGYLNAEPVSTSTVFYGEDVAGIYCVSTLHEARGKG
                     IGAALTLQPLQEARDLGYRVGVLQSSDMGFNVYKRLGFRHLCQIEYYYLSMQSAI"
     gene            6829..7443
                     /locus_tag="VFQ23_04515"
     CDS             6829..7443
                     /locus_tag="VFQ23_04515"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HET9905873.1"
                     /translation="MVRKTQTGYLVLADISGYTSYIAQTEIEHADMALSFLLETLVEQ
                     LSSLLTICQLEGDAVFAYIEESTLAEAKSLLGLIDQTYLAFREKALALYAGATCPCRA
                     CKALPTLDLKFMVHHGDYLIQNVAGASHLLGTDVNLLHRLAKNHVSESTGWKGYALFT
                     DHVLNQMRHSKDNFVKQSESYEHLGEVEVYCMDMHRRYQEMKEN"
     gene            7461..8288
                     /locus_tag="VFQ23_04520"
     CDS             7461..8288
                     /locus_tag="VFQ23_04520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9905874.1"
                     /translation="MFNVKEAPWKVILVFLLVSTCMTALINLVLFPGPLFDPISNATS
                     YLIDGTLQANLINILVFALIIFGWGKLRPIDVGLDWGKLAQGLCLTALLWLGMQGIVL
                     LINWINGDIHLDPAWSERGVTRMIGGLIAQLLGNALFEEMEYRGFYLSQFYLKLKNQS
                     GRQRLFWAVLSMLVLFVLSHIPNRIFFGYSLADIPLDFALLFAWGLFFTAIYLISGNL
                     FLAIGVHALSNRPTLITEATFPPQALLFILTWILLAVLSFQKRQRRLQGEVEAFAEP"
     gene            complement(8396..8905)
                     /locus_tag="VFQ23_04525"
     CDS             complement(8396..8905)
                     /locus_tag="VFQ23_04525"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET9905875.1"
                     /translation="MMFEIGDRVVHPQHGVGQIVKLEAREFESGVVRQYYEVTMSGSS
                     TLWVPVDLETLGLRKPAARTEIARCREILIARALPLVDEARSRQANLASRLKKGTITT
                     FCEVVRDLYAFGEHKSLYGTIAGFYKVTKDVLCQEWAIVEGITYLDAVQEVDILLTRG
                     RETMKTAGA"
     gene            complement(8915..9220)
                     /locus_tag="VFQ23_04530"
     CDS             complement(8915..9220)
                     /locus_tag="VFQ23_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET9905876.1"
                     /translation="MEAKLYVGNLSYETTEQELRELFMQAGNVVSVALIKEPGTNRSK
                     GFAFVEMSSRNEVQKAISMFNSYTLNDRQLKVNVAQPREERGGFRQQRGGNNQRRRF"
     gene            9510..9785
                     /locus_tag="VFQ23_04535"
     CDS             9510..9785
                     /locus_tag="VFQ23_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET9905877.1"
                     /translation="MEAKLYVRNLDKSINAEELNMLFAQAGDVLSVDIIRDRATGESR
                     GFAYITMSAQSEADKAISLLNDYSLRDQSLHVNLAKPRIQRGLGKVH"
     gene            9787..10233
                     /locus_tag="VFQ23_04540"
     CDS             9787..10233
                     /locus_tag="VFQ23_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9905878.1"
                     /translation="MFSFTLNTKIYRPLQEVFKFVATPENDFEWQYGTLTSTQVSGGA
                     IGVGTLFRTKGHLIRRLVEGVYEITEYETNQKYGFKSLSGPMRLHTLYTFEISYGSTR
                     INMFFQVSPEDFFKANDASVEKSVKKQCRENLALLKSILETRHIVR"
     gene            10424..10858
                     /locus_tag="VFQ23_04545"
     CDS             10424..10858
                     /locus_tag="VFQ23_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9905879.1"
                     /translation="MIQIISSTLISQPIKRVFEFICTPENDFQWQYETLASTQISEGV
                     MRVGVCFRSDGHLMGLRTQSTFEVIEYEPNKRYGFKSLSGPLQSITAYTFEIARRYTQ
                     VKQSTQANVVNRIELNENVLEKKMKRQIKENLAMLKKILEVG"
BASE COUNT         2871 a         2669 c         2685 g         2719 t
ORIGIN      
        1 catgggttgc cagccagtac ggcaaagagc aacagaatga tggacagaaa atacggaatg
       61 gtcaacaacc aggcgatcat ctcacggcca cggttcatgg cgaagccgga ggtaaagagc
      121 agcaaaggca ggagaaacgc gggggagcga atgatcggaa acccgctcag gtaccaagca
      181 aatggaaatg ccagaatggc agcagcaacg agaaaacgat aatcacgcct gatcagacca
      241 ataagggcca gaataatcgt gcccacgatc gcgggccacc caacgagggc aatgaggagg
      301 aaatcaagca tattattcga aaaactttat tttctgtttt ttggtactcg ataaataaat
      361 agctcagcta acatggccat tacgagaaaa taaacaaata aacccaaaaa gaaaaaattc
      421 gccagatatt cttggttaaa cactaaagag gacaatattc caagaattcc tatcgcggca
      481 cctaccccaa aaatagtaat aaaacgaaag tttttttcag taaacttaat tttctcttct
      541 ctgttcatac tctgaagata ccttgcaaaa cgcagtcgtg atctagtacc aataactgca
      601 attaccgcca caaaccccat gataatgcca aagcaaatta cagttccaag agtgttcata
      661 atttgatcct cacaatcgtt gtccaaatta tgacgccaat aacctcttcg ctgccttatt
      721 atgtttctcc gctaacttcc gttcatcaac agtgaccaac tgtccttctt tgaccacgaa
      781 cttgccaccg accactgtgt aatctgcatt gacgggctgc ccaaagacca ccgcagccac
      841 gggatcatgc atccctgcgt agccgatgcg gttgagattg atggcgaaga agtcggcaca
      901 tttcccagct tcgagcgagc cgatatcatt tcgtccgacc acggctgccc caccgcgcgt
      961 ccccagatac agggcttcgc gcgccgtcat caatttgcgt tctccctcac cctcccctct
     1021 cccgccggga gagggtgtag cggaaaaacc cgtaatccct tctttgacgc gcgacaataa
     1081 caatgcgttg cgaacttcag ccaggagatg tgaaccatcg ttagaagcgg aaccgtcgac
     1141 gccgagtcca acaggcacac ctgccgcgcg gtattccttg agtggtgcga tacctgaagc
     1201 gagtcgcatg ttcgaggtcg ggcagtgcgc tacaccgcag ttatgtttgg caaagacttt
     1261 gatctcctca tcgttgaccc acacagcatg cgcaaaccag acatcgtcgc ctacccaatc
     1321 gacttcctgc atgtacccca cgggacgatg tccgaacttt tgcaaacaga attgttcttc
     1381 atcttcggtt tctgccaaat gcgtgtgaag atgaacgcca tattcacgcg ctaatttggc
     1441 ggattgtttc attaaatcag ttgtcacgga aaatggagaa catggtgcga gcacgatctg
     1501 caccatcgca ccaggtttgg catcgtgata cgcttcgatc agcctctggg agtctttcaa
     1561 aatggagtct tcgtcgtcca caacggaatc aggcggaagc ccaccctgag aggcaccgag
     1621 cgacattgag ccgcgcgagg cctgaatgcg gagtccgatc tcctgcgcgg cggcgatttc
     1681 atcgtcgagt ttggatccgt tcgggaagag atagaggtgg tcagaagctg tcgtgcagcc
     1741 cgataacgcc aattccgcca gcgcggtctg agtcgaggta aagatatcct ccggccggat
     1801 gcgcgcccag atgggataga gagtcgtcag ccagttgaag agattggcat cctgcgcggc
     1861 agggacggca cgcgtcaagg tctgataaaa gtgatggtgc gtgttgacca gccccggcag
     1921 gacgatgtga tccttgagat ctagcacttc gtcggcggct tgaggcaggt cagacatgcg
     1981 acccacctct tgaatgaggc cgcgtcgaat aaaaagacca ccttccgcga tttcggtctg
     2041 gtggtcgtcc atcgtaacaa tgtgagcgtt tttgataagg agggtggaca tttcggtaat
     2101 tgaatgtgaa aggtagaaat tgcctagatg tctgacaagt cgagtgtagc aaaaaaggga
     2161 aaggatgtca attgagattt tgacgagcaa cttataataa acggaattaa tgctaaaagg
     2221 aaaagcaaag tgactataaa aatttctgtc tatattgcta ccagcctgga tggtttcatc
     2281 gcccgcagga atggtgacat tgactggctc accggtggag aaggcggcga agattatggg
     2341 tatgcagaat ttatgtctga tattgaccat attgtgatgg gaagaaacac ctacgaaaaa
     2401 gtattgacct ttggcggctg gccctatgcg aaaaaggtca ttgtgctttc cagcaggaat
     2461 ttatccattc cacctgaact atcagacaaa gtagaagcgc taaatttatc tccccgtgaa
     2521 ttgataagtg aaatggatac gcgcattgcg aaacatatct atctcgatgg aggtgtcacc
     2581 atccagcgct tcctccgtga aggtctcgtg gatgaattga cgattacaac catccctatt
     2641 ttgattggag aaggtctgtc attatttggc gaattagaga gggatgtaaa acttgagctg
     2701 ataaagtccg agtcatttat gaatggtttt gtgcaaaata ggtataaagt tcttagatag
     2761 aaagattagt tatctgacaa acattgcata ataaaacatc aggcattttc aaacccttat
     2821 gggaaagtcc gggcgggcat tgtttctccc tcaccctgcc ctttacagag cgctacgcag
     2881 tctcctaaag gagaggggta tagaggcgct ttataatccc ttcatgtcaa gaccgcccgg
     2941 ccaaatcata caacctactc ccgcggcgta aatgaattca cccgcatggc caagcccagc
     3001 cggatgggaa ttctcgggtt attttaccaa tccccgtatt tgaataagtc ccgagcggtc
     3061 accctggaaa agggtagtag tataatttca ttcgaggaac gaaaatgacg atccacacct
     3121 ctccaagctc tgtcctgact aaagtggcaa agtcacttgt cgcggcaaaa cgttattcca
     3181 ccacagaaga agccttgtgg gatatggcgc tttccacagt gcgcggcaaa gtagcttact
     3241 accgtcgtcg aatccgcagg ctggagaaca aacatggggt ggattttgat aaatttacgg
     3301 cgcgtctgaa aggaaaagcc acacctgccc aggaggacga ttggctgatg tggaagtctg
     3361 cccgttccat gttaagggat tggcaagcaa cgtacaaaga cctgctccgt gaacaacctg
     3421 gttgacgata ttctggaact tctacagaca cacgcgttgg tgaaagccac gcgtgttgtt
     3481 tattatgacg agacaccatc tggcaagctc gaggtaaaga ttcgttctga attgcccaga
     3541 aaccataagc ttcaaatttg gattcatctt gagactgatt cgctggatta cgcctaccaa
     3601 gtctttacaa ccattccgct attaaggtgg gataactctc cgcattatgg tcatcttaaa
     3661 tctgctccac atcatttcca caatgaaaat gggcgggttt acgattcgcc gctcactgga
     3721 aatattaaga aggatttgaa agtagttttg caagaaatca aggattggat ggaagttcag
     3781 caagaggagc acaaatagat tcagggttct gccagcccga cggttaaccg tctaggcaga
     3841 caaagagggg acctcacccc cgcccttcct acggagcgct tcgcagtctc ctaaagggag
     3901 agggggcatg aagccgcttt ataatcaatt cgtgcaaaaa tcgcctagcc aaatcatcaa
     3961 aacctcacgc ggcgcgattg aatatgcctt gcataacccc aatggaggtg caacggtcat
     4021 cctctgtcac agtacaggag gtgggtatga tcagggactc gtattggctc agttgctaga
     4081 cggctttcaa ggaatcgcag tgtcgcgggc cgggtatttg cgcactcccg tagaaacagg
     4141 tgcatcgcca gctgaattgg cagatgccta cgaggaatta ttggattcat tggaggttga
     4201 gaaagctgcc atccttggct tatccggtgg aggcatgtct gcggcgcatt ttgcgttcag
     4261 gcattccgat cgctgttggg ggttggtatt ggcagatgca atcacgaaag cgccacctaa
     4321 gtcatcggca agaattgtgg caacagcaaa ttcattgcca gatggaatcg cgtggctgtt
     4381 aacaaggctt gcaatttata tcgggttgcc attgatggtt cgcgacgcgg aaacgcgctc
     4441 catgatgaga gtgttcttcg agaacaaccc gatttcggag cgccgcgcag gggtgcggaa
     4501 tgaccttatg aacgtaaacg caatgaatga atttccgtgg gaggagatct gtgtcccaac
     4561 tttattgatt catggtgaca aggacaatct catccccgtg gaatatagcc gcgaggtcgc
     4621 gcgccgcatc tcaaatgctg agctggtcat tgtcaaaggc ggagggcacg aatgtttggt
     4681 gtcccatcag cgcgaggtgg ctccgatgct gaattcgttc ttaaaaaaat atatatcgag
     4741 cccacccgac aggtagcgtc ggggcaggct catttccggg tgcatgtctt aaacagggtt
     4801 tcgtgtctat aaaaaattcc cacctttttt ctaaatgagc tatttcttag gaaccttcat
     4861 cccttccctg cctttttcaa gcagggcatc cacttcctgc acagcttctg cataggtgac
     4921 tccttccacg acggcccatt cctgacagag cacatctttg gtcacacgga agaacccggc
     4981 aatcgtccca aacaatgact tgtgttcacc ataggcatag agatcgcgga ccacttcaca
     5041 aaacgtggaa atcgtccctt ttctgaggcg agctaacagg tctgactggc gggaacgtga
     5101 atcttcggga agatgggcgg cgcgggatac aagaatcttg cgacagcggg caatcgcact
     5161 tctcccggct ggtttacgca gtccaatggt cccgagatcc acaggcgccc agatcgtact
     5221 gccacccttc atagaaatct cgtaatacgt gcgagttacc ccggtctcga attccttatc
     5281 ttctagcttc accacttgcc caaccccgtg ctggggatga acaacctggt caccgatttc
     5341 gaattgcatg gtgatggttt agtaccgacg acgattatca ttacgaccgc gctgctggcg
     5401 gaagccgcca ccacgttctt cacgcggttt cgcaacattc accttcaact gccggtcatt
     5461 cagggtatag ccgttaaaca tgctgatagc cttctgaact tcactttgtg aggccatttc
     5521 tacaaagcca aaccccttgg aacggttcgt tccaggctcc ttgatcaacg ccaccgacac
     5581 tacattcccg gcttgcatga acaattcgcg taaaccctgc tcagtcgtat cgtaggacaa
     5641 attcccgaca tatagctttg cttccatttc ggactccttt ttgtggatca ggggatgaaa
     5701 aagaaaaccg ccagcccaat aataggggtg acggtgagat aacacgcttg accttctatt
     5761 aagatgggga cagtatatca cactcaagcc agcagcctga ttcaggcatt ccacttgccg
     5821 gtctggccct tcgtttatca acctgaatgg tcttatcata taatttcgaa ttaaaatgat
     5881 tcgtatccgt tatagtcatt gcagaacgaa ggagaaaagc atgaatcaca tccaggctga
     5941 tatgtctgat gaagcgttag ttaccgccat ccgtaacaat atgtgcgatt tctttcgcca
     6001 tgtcagtaga agtgatctgc aagaacactt tgagagtcag caatttacac gctggcactc
     6061 tccacttcca catccctggt tcaacggcgt gctgagttcc cgtgcttatg aagagggcga
     6121 tgatgccttc attgaagcca cgatccagta cttccgcgag aagggaatcc tgtcgttcac
     6181 atggtggatg gaaccgcacg tgagtccacc ggcctgggaa caaatccaat cgaggcatgg
     6241 ttttcgttat tccaacgata cgccgggcat ggcaattgac ctcaatgacc tgaacgactc
     6301 gccgaggctt ctggaaggct ttgaaatccg tgttgtcaac gatgaagaga cactgcatgc
     6361 ctgggtaaaa acattcatac atggatatgg cttgccgtct cactgggaat cgctcacttt
     6421 cgacctctgg gtccagcttg ggctcgatct ccctatacgc aactatcttg gatatttgaa
     6481 cgctgagccc gtttctacat ctacagtctt ttatggtgaa gacgttgcag gaatctattg
     6541 tgtgtccacc ttgcacgagg cgcgaggcaa aggcatcggc gccgcgctta ccctccaacc
     6601 tttgcaggag gcacgtgacc tgggttatcg tgtgggtgtg cttcaatcct cggatatggg
     6661 atttaacgtc tacaaacggc tggggttcag gcatttgtgc cagatcgaat attattatct
     6721 gtcgatgcaa tcagcaatat aaagggaaaa cctgtggtaa tgttattcag accgaaattg
     6781 tcgaaatagc atgagaatag gaatatgatt ggctccgagg agtaagtgat ggtacgaaaa
     6841 acacagaccg gttatttggt cctggcagac atctcagggt acacctctta catcgcgcaa
     6901 acggagattg agcatgccga catggcgctc tcgttccttt tggaaacgct cgtcgagcaa
     6961 ctgagcagcc tgctgacaat ctgtcagttg gaaggagatg cagtctttgc ctatatcgag
     7021 gaaagcacct tggcggaagc caaatccctg ctggggttga tcgatcaaac ctacctggca
     7081 tttcgtgaaa aggcattggc tctctacgcg ggggcaacct gcccgtgccg ggcatgcaag
     7141 gcactcccca ccctcgacct gaagttcatg gtgcatcacg gcgattacct gatccaaaat
     7201 gtagctggcg ccagtcatct gctggggacg gatgttaatc tccttcaccg acttgcgaag
     7261 aatcacgtct ctgaatccac aggctggaag ggatatgcgc tttttaccga tcacgttctg
     7321 aatcagatga ggcatagtaa agataacttt gtaaaacaat ccgagtccta cgaacatctg
     7381 ggggaagtcg aagtgtactg catggatatg cataggcgtt atcaggagat gaaggaaaat
     7441 tagccggaga ggcaaaccga atgttcaacg tcaaagaagc tccgtggaag gtcatcctgg
     7501 tctttttgtt ggtcagcacg tgcatgacgg cgctgatcaa tctggtgttg tttcccggcc
     7561 cgttgtttga cccaatatca aatgcaacat cctatctgat cgatggcacc ctgcaggcaa
     7621 atctgatcaa catccttgtt tttgccctga tcatctttgg atggggcaag ctccgcccga
     7681 tcgatgtggg cctggactgg ggcaagctgg ctcaggggtt gtgtttgacg gccctgctgt
     7741 ggctggggat gcagggcatc gtcctgctca ttaactggat caacggtgat attcacctcg
     7801 accctgcctg gagtgaacgc ggcgtgactc ggatgatcgg cggtttgatc gcccagttgc
     7861 ttggcaacgc cttgtttgaa gaaatggaat atcggggttt ttatttgagt cagttctatc
     7921 taaaactgaa aaatcaaagt ggacgccagc ggctgttctg ggcggtcctc tctatgcttg
     7981 tcttgttcgt gctctcccat attcccaacc gcatcttttt cggctacagc ctggcagata
     8041 tcccactcga ctttgccctg ctttttgcct ggggattgtt ctttactgcg atctatttga
     8101 tctcgggtaa tctgttcctg gcgatcggcg tgcatgcact gtcgaaccgc ccgacgctga
     8161 tcacggaggc gacctttccc ccgcaggcac tgcttttcat tctgacctgg atcctgttgg
     8221 cagtgctttc ttttcaaaag cggcaaagac gcctgcaagg tgaagtcgag gcttttgcag
     8281 aaccctaaga cccttcttaa tgatttactt cctaacggtg tgataggtgt agttaggaag
     8341 tgcgaaagtt tatggcatgg agatgcaggc ttgattaatt cgtatcttcc accaattatg
     8401 ccccggctgt tttcatggtt tccctgcctc ttgtcagcag gatatcgacc tcctgcacag
     8461 catccaaata tgtgatccct tccacgatgg cccattcctg gcagagtacg tcttttgtca
     8521 ctttatagaa ccctgcaatc gtcccgtaca aggatttgtg ctcgccaaac gcatatagat
     8581 cacggacgac ttcacaaaac gttgtgatcg tccccttctt gaggcgagat gccaggttcg
     8641 cttgccggct gcgcgcttca tcaacgaggg gtaacgcgcg cgcaatcaga atttcgcggc
     8701 agcgcgcaat ctcagttctc gcggctggct tacgcaaccc gagggtttct aaatctacag
     8761 gcacccacaa ggtgctactg cccgacatag taacctcata atattgccga actacaccag
     8821 actcgaattc cctggcttcc agtttaacga tctgcccaac tccatgctgg ggatgaacaa
     8881 cgcgatcacc aatttcaaac atcatgttgc tcgattaaaa gcgacgacgc tggttattgc
     8941 caccccgctg ctggcgaaag ccaccacgtt cctcacgcgg ttgtgccaca ttcaccttca
     9001 attggcggtc attcagagtg taactattga acatgctgat cgccttttgc acctcattgc
     9061 gtgagctcat ttctacaaaa gcaaacccct tggaacggtt ggtaccaggc tccttgatga
     9121 gggcaacaga gaccacattc ccggcctgca taaacaactc gcgcaattcc tgctctgtgg
     9181 tttcatacga caaattccca acatacagtt tcgcttccat accgaactcc tttatcgtgc
     9241 gttaaataag aaaccgccaa ccctaccata gggttgacgg cgctagaagc acactcgacc
     9301 ttttataaga taatttgatt gtatcacacc cggctgtggt tcccttattc tgtgatgaga
     9361 agcgcctgct gataacgcgt ttcgaagtat ggtagactct atccaacagt tgagaaggaa
     9421 taactggtat tgcgtgacat tttggccgcc gtccctgtta tagggtggcg gctttttctt
     9481 tatcagataa acgggagaaa ctagtaaaaa tggaagccaa gctctacgtg agaaatctgg
     9541 ataagtcaat caatgcggaa gagttgaata tgctgttcgc acaagccggg gatgtactat
     9601 cagtcgatat cattcgagat cgggcaaccg gagagtccag gggttttgcc tacattacaa
     9661 tgagcgcgca aagcgaagcg gacaaggcca tcagcctatt aaatgactat tccttacgtg
     9721 atcaatcgtt gcatgtgaat ctggcaaaac cgaggataca gcgtgggttg ggtaaggtac
     9781 attgaaatgt tcagtttcac actgaacacg aagatctatc gccccctcca agaggtcttt
     9841 aagtttgtcg ccacgcctga gaacgacttc gaatggcaat atggaacgtt gacgtcaacc
     9901 caggtatcag gcggggctat aggtgtagga actctttttc gtaccaaagg tcatttgatt
     9961 cggcgtctgg tcgaaggcgt ttatgaaatt accgaatatg aaaccaacca aaaatacgga
    10021 tttaagtctc tctctggtcc aatgcgcttg cacactttat atacctttga gatttcgtac
    10081 ggcagcacca ggatcaatat gttctttcaa gttagcccgg aagatttttt caaagccaat
    10141 gatgcctcgg tagaaaaaag tgttaagaaa cagtgtagag aaaatcttgc cttattaaaa
    10201 agcattttgg aaacacgcca tatcgtccgc taatagctgc atacaagttt tgctttttcc
    10261 agcagttgga gatcagacgg tagggcaaaa gtttcgtcag gtactgtctg aagcaatgga
    10321 aaaagcacag aaccattcaa agtattactg atatgctgaa cgacgaactg ttgcgctttc
    10381 cagtatgacc tttaccagaa ccctatatgc cggagacggg taagtgatcc agattatttc
    10441 aagcacgctt atctcccaac ctatcaaacg ggttttcgaa ttcatatgta cccctgaaaa
    10501 cgattttcag tggcagtatg aaacgctggc ttcgacccaa atctccgaag gtgtcatgcg
    10561 cgtgggggtc tgttttcgaa gcgacggtca tttgatgggg cttcggaccc aaagcacgtt
    10621 tgaagtgatc gaatatgaac caaacaagcg atatggcttc aaatctctgt caggcccttt
    10681 gcagtcaatt actgcctaca cgtttgagat cgcgcgaaga tacacacagg tgaaacaatc
    10741 aacacaggcg aatgttgtta acaggatcga actgaatgag aatgtgctgg aaaaaaagat
    10801 gaaaagacaa atcaaagaaa acctggcaat gctcaaaaag attctggaag tgggataagc
    10861 gaagaatata tttcttaatc tgtgtgctga taatttatgg ggttggcgta ggcattgcgg
    10921 aaaatattgt gagtggcggc acat
//
LOCUS       DASTJP010000148         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_767183, whole genome shotgun sequence.
ACCESSION   DASTJP010000148 DASTJP010000000
VERSION     DASTJP010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767183"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1912)
                     /locus_tag="VFQ23_04550"
     CDS             complement(176..1912)
                     /locus_tag="VFQ23_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905880.1"
                     /translation="MDNKIYFHQELPNVVDANLRRVQVVFRILACGLGVLHVYAAITS
                     QSMNADGIAYLDIGDAYFRGDWANAINPVWSPLYSWILGIANFVIRPSIQWEFPVIHL
                     VNLGIYLLALSSFEFMWKRVRTYEPSTELHAVSDFWWWIIGYLLFIWISLSFIEIWAV
                     TPDMLMAAFVFWGGGLIAQIRAGDVSFRNYLYLGLVLGLGYLSKTFMFAMAIIFLGTS
                     FVLAHRSKNSLLYAPAAIGVFILISLPFILLISEKKGKITIGEAATVTYLRYANGMPF
                     PHWQGDPRKGFILTHPSRIVHHSPVVYEFGEPIGGTYPISTDPSYWYEGIKPQFDFGG
                     LLGRLLANSIILAKLFLQRQGIFLACVVALYMMGKRQKYPIEETLRRWALLIPAVVAF
                     GLYATVLVEERYVGVFILLFWADILGNVRLPNIPANGLWLGVLSSIAAIGLLVNIIMF
                     NLDGFTSLLGASSQTSLGEVITPAARPIAVAQTLQELGVRQGDKVGVIGYAYDSFWAR
                     LARVRIVSEMLETDAVTFWESDTILQHSVLDAFASTGAKAVIAEYVPADAALSDWHQV
                     GESNYYIFVFRE"
     gene            complement(1905..2042)
                     /locus_tag="VFQ23_04555"
     CDS             complement(1905..2042)
                     /locus_tag="VFQ23_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905881.1"
                     /translation="MKRFLNSPYLIAESIAANSVTMTVGLYTPLEHLMVRGDIFEIIR
                     G"
     gene            2386..3330
                     /locus_tag="VFQ23_04560"
     CDS             2386..3330
                     /locus_tag="VFQ23_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HET9905882.1"
                     /translation="MSRNMREKHPVHWILYVALVLTGVLTGMLIQRYVGLGNMLRAIG
                     VAYPTRVPGADSISSPSIVIPKEHQGQMHLFILAGQSNMVGWSPIPAGEPTDSRIYLF
                     GKDYRWRIAKHPIEDASNQVDMVSENRLAGFGPGMDFAFASLERNPDIIIGLIPCAKN
                     SSGIIQWQRDLSDQSLYGSCLKRIRAASSMGEISGVLFFQGETDAQDPVLYPEPHPQP
                     FDWGSLFTAFITDFRTDLNEPELPVVFAQLGADLTSLDFPNWKTVQAQQSAIDLPLTA
                     MITTDDLPLLDGLHFTADSYRIIGRRFADAYWTLAQQP"
     gene            complement(3339..>4089)
                     /locus_tag="VFQ23_04565"
     CDS             complement(3339..>4089)
                     /locus_tag="VFQ23_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905883.1"
                     /translation="SFMPYLLIKKDFWLGWFSNVVNVAEFMPLFLAILGFFLLRNRKV
                     QFLIVGLAIGFLVFSMAFTYHIHTHPYYHIQLFPIVGLCMAPIVVNVLQSLRESIGKL
                     FWIPIVGAFLFAFYVGYTEVRNSLYQTNLENPTVAREIGRIIQHSSRTVFVAFYYGVP
                     LEYYGEFGGAPWPVRIEEEFYRRQGERELSIPERIDSLGFVPEYYVITHFNLYNRKHQ
                     DLQAYLENNCSVLKETEAYLIYSKCGPVLAN"
BASE COUNT         1189 a         1015 c          911 g          974 t
ORIGIN      
        1 cggccaggcg cggctcatga gaatccagaa aaggcactgt tccaagactg gtcggccgag
       61 tgttgggatg acaagactga tcccctcgag aacaggtctg gtctcgaatt tcatcgtttc
      121 aattgaactg gagcacgtcc attctgcttt ttcaattaat gggttcaatg atccatcatt
      181 ccctgaaaac aaaaatatag taattacttt cacccacttg atgccaatcg ctcaacgcag
      241 catcggccgg tacgtattcg gcgatgactg ctttggcgcc agtactcgca aatgcatcca
      301 ggacactatg ctgaagtata gtgtcgctct cccagaacgt cactgcatcc gtttcgagca
      361 tttccgagac gatcctgacg cgcgcaagcc tggcccagaa ggaatcataa gcataaccga
      421 tcacgccaac cttgtcgccc tgtcttacgc caagctcttg aagcgtttgc gcaaccgcaa
      481 tcggccgggc agccggcgta atcacctccc caagactagt ctgcgatgaa gcaccaagga
      541 ggctagtaaa accatccaga ttaaacatga taatgtttac cagcaagcca atggcagcaa
      601 ttgaacttaa cacccctagc cataatccgt tggcaggtat gtttggcagg cgaacattgc
      661 caagaatatc tgcccagaac aaaagaataa atacacccac ataacgctcc tctacaagca
      721 cggttgcata caatccgaag gcaaccacag ccggtatgag caaggcccag cgtcgcagcg
      781 tttcttcaat tggatatttt tgtcttttgc ccatcatgta caatgccacg acacatgcca
      841 aaaatatccc ctgcctttga aggaataact ttgcaaggat gatgctattg gccaataatc
      901 gcccaaggag acctccaaaa tcgaattgag gcttgatgcc ctcataccaa taagaagggt
      961 ctgttgaaat gggataggtt cctccaatcg gctcaccaaa ttcatacaca acaggtgaat
     1021 gatgaacaat tcgcgacgga tgtgtgagaa taaatccctt ccgcgggtca ccctgccaat
     1081 gtggaaaagg catgccattg gcataccgta agtacgtaac cgttgctgcc tctcctatcg
     1141 tgattttgcc ttttttttca gaaatcaaca gaataaacgg aagactaata agaataaata
     1201 cacctattgc cgcaggcgca taaagaagcg aatttttact gcggtgagcc aagacaaagc
     1261 tggtccccag gaagataatc gccattgcaa acataaaggt tttggataaa tatccgagac
     1321 caagcaccaa tccaagataa agatagttac gaaaacttac gtcccccgcc cgtatttggg
     1381 ctatcagtcc tcctccccaa aaaacaaacg ccgccatcag catatctgga gttacggccc
     1441 agatttctat aaagctgagc gaaatccaga tgaaaagaag atatccaatt atccaccacc
     1501 aaaagtcgct aacggcatgc aactcggtcg atggttcata ggtccggact cttttccaca
     1561 taaactcaaa actactgagc gccaacaaat agatccccag atttacaagg tggatgactg
     1621 ggaactccca ttgtatcgag gggcgaatga caaagttcgc aattccaaga atccaggaat
     1681 ataacgggct ccatacggga ttaatcgcgt ttgcccaatc cccacgaaag taggcgtcgc
     1741 caatgtccaa ataagcaatg ccgtccgcat tcatggactg gcttgtgatg gcggcataga
     1801 cgtgtaacac accgagccca catgccagaa tgcgaaagac gacctgaacc cttcttaagt
     1861 ttgcatcgac cacgttgggt aattcctgat ggaagtaaat tttattatcc acggataatc
     1921 tcaaagatgt caccacgaac catgagatgt tcaagaggag tataaaggcc aacagtcatg
     1981 gtaacgctat tagccgcaat gctttcggcg atcaaatagg gggaattcag aaatcgtttc
     2041 attcgaattg tggataattg gctggaaatc aaccccaaca cgaaaaaaaa catactagtc
     2101 agagcgctag ctctgctctt cgatgataaa tgatcgagag tctccctggt gtgatccgat
     2161 gaccctcatt agcaaaaatt accatccagc cgttctggca gagatcaaat cagataggcg
     2221 gcaaaagacg gcggagcctg ctccttgcgg tatgcaggta cgacaaaaga aaagtcgatt
     2281 ctcagatata gccttcagct acataatccc tttgaatatg atgaagaccg tattattcga
     2341 tataatacat ttgagacatc atagaaagaa accatctaat agcgcatgtc cagaaatatg
     2401 agagaaaaac atccggtcca ttggatttta tacgtcgcgc tcgtgctgac cggggtgttg
     2461 accggcatgt tgatacaaag gtacgtgggg cttggaaaca tgctgagggc aattggtgtt
     2521 gcctatccga cccgagttcc tggagcagac tccatctctt cacccagtat cgtgatcccc
     2581 aaagagcatc aggggcaaat gcacctgttc atcctggctg ggcaatccaa catggtaggc
     2641 tggtcgccta ttcccgctgg tgagccgact gattcccgca tttatctgtt cggaaaggat
     2701 taccgctggc ggatcgccaa gcatccgatt gaagatgcgt cgaatcaggt ggatatggtg
     2761 tctgaaaacc gcctcgccgg atttggtcct ggcatggatt ttgcgttcgc tagccttgaa
     2821 cgtaacccgg atattataat tggccttatc ccatgcgcca agaattcgag cggaatcatc
     2881 caatggcaga gagatttgag cgatcaatcc ctatatggtt cctgtctgaa acggatccgc
     2941 gccgcctcgt caatgggcga aatttcaggg gtattgttct ttcaaggcga gacagacgca
     3001 caagatccag tactatatcc tgagccccat ccacaaccgt tcgattgggg atcattattt
     3061 acggcgttta ttaccgattt tcggaccgac ttgaacgagc ccgagcttcc cgttgtcttt
     3121 gctcagcttg gtgctgatct aacatctcta gattttccga actggaagac cgttcaagct
     3181 cagcaatctg ccatagattt accgttgacg gccatgatca ctacggacga tctcccgctt
     3241 ctggatggac tccatttcac cgccgatagt tatcgaatta ttggaaggcg ctttgcagat
     3301 gcttattgga ccctggcaca gcagccatag aatgtgaatc agttagcaag tacaggtccg
     3361 cacttcgagt aaatcagata agcttctgtt tctttaagca cagaacaatt gttttcaaga
     3421 taggcttgca gatcctgatg cttacggtta tacaagttaa aatgtgtaat gacataatat
     3481 tctggcacga atcccaaact atcaatgcgc tccgggatcg ataactctcg ttcaccctgg
     3541 cggcggtaga actcttcctc aatacgaaca ggccaaggcg cgccaccgaa ttctccataa
     3601 tattccagag gtacgccata ataaaatgct acaaatactg tacgcgaact atgttggatg
     3661 attcttccaa tttcgcgtgc aacggtggga ttttccaaat tggtttggta caagctgttt
     3721 ctcacttcgg tatacccgac gtaaaaggca aacaggaagg cacccacaat tggaatccaa
     3781 aacaatttcc caattgactc cctgagagac tgcagaacgt ttacgacaat gggcgccata
     3841 catagtccta caatgggaaa aagctggatg tggtagtaag gatgtgtgtg gatgtgatat
     3901 gtaaaggcca tgctaaacac caggaagcca atcgccaacc cgactatcag gaattggacc
     3961 tttcggttgc gcaaaaggaa aaagccgagg atcgctagaa acaacggcat aaattctgcc
     4021 acattcacaa cattactgaa ccagcctaac cagaaatcct tcttaatcag caagtatggc
     4081 atgaagctg
//
LOCUS       DASTJP010000149         8559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1182841, whole genome shotgun sequence.
ACCESSION   DASTJP010000149 DASTJP010000000
VERSION     DASTJP010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182841"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VFQ23_04570"
     CDS             complement(<1..874)
                     /locus_tag="VFQ23_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9905884.1"
                     /translation="MPNSKLVPYLEVLLAVIAWGASFIATKIALQDVSPISIVWLRFT
                     MGVVILGIAVAIRSQFALPKKSELGYFALLGFLGITFHQWLQSNGLQTSEASTTAWIV
                     ATTPVFMALLGWVILREGLPLLKIFGIILAAFGVLLVVTDGNLASISVGEFGAPGDIL
                     ILASAFNWAVFSALSRRGLKTYSATLMIFYVMTFGWFFTSLAFIAGRNYLEIPNLSFN
                     GWIGITFLGVFCSGLAYIAWYDALQALSTAETGVFLYIEPLVAVVVAFFLLDEAITWV
                     SLVGGAVILVGVWIV"
     gene            1021..1827
                     /locus_tag="VFQ23_04575"
     CDS             1021..1827
                     /locus_tag="VFQ23_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905885.1"
                     /translation="MESSDVSYASEKLTQLKSEYHVDVIADWGMGSDGKWHNGTWSKE
                     ELDRLHRSISLLTGIMKGADHFVENLGGVTVVKEDIGLHGGQAQAHLVKFNSKSSFSA
                     WTVVHEFAHAWDANFDWKLSVALERYTGGYTRPWLAWFKKLIGMSDSRFFKHENKPGK
                     RGRKPGCNAAGYFYGDKPSGSNWMFNRLEDFAESVAMYIGWARSNDLSNWAEARIKRY
                     LLEDGVKDKTFGTDYWKYYSKFFYPDRGDYTKTKRWKFVEELVKGRIKIA"
     gene            1885..2550
                     /locus_tag="VFQ23_04580"
     CDS             1885..2550
                     /locus_tag="VFQ23_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9905886.1"
                     /translation="MEITWYGHSCFRLTERNYATVVTDPYDNKSIGYSALRLKSDIVT
                     VSHDAPGHNNTDAVKGISHVIDGAGEFEIGGVFITGVQTDGGGSSRKKSKDKEDGPRN
                     TIYVFDYDGITVAHLGDLKQTPTQSEIESLGTINVALIPVGGGGGLNAAKAAEVISLI
                     EPNLVIPMHYSTPDVKLSLDSLNKFIKEMGLSTPDSQPSLKVTRSGLPNETKVVVLDY
                     QSS"
     gene            2555..2857
                     /locus_tag="VFQ23_04585"
     CDS             2555..2857
                     /locus_tag="VFQ23_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905887.1"
                     /translation="MAVKVLMTWDIAGERDQDYFEFIIGEFIPGVQRLGLQPAEAWAT
                     IYGSYPQIQVGLLATDVSQARQILDSSDWGVLQDRLFNYVKNFSYKVVPARGGFQF"
     gene            2924..4000
                     /gene="mutY"
                     /locus_tag="VFQ23_04590"
     CDS             2924..4000
                     /gene="mutY"
                     /locus_tag="VFQ23_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET9905888.1"
                     /translation="MSRLSSRLLDWYHKNKRTMPWRVLQPDPYRVWVSEIMLQQTRVE
                     TVIPYFEKWMRLFPTIQALATASEHDVLNAWEGLGYYSRARNLHKAAKIVAEKYKGKL
                     PSDADALRNLPGIGRYTLGAISSIAFGLDVPALDGNIKRVYARLFDVVDPVDSTVGEK
                     ILWDLAETHLPEGQAGDYNQALMDLGATICLPKNPRCLICPLMKVCKARVNGTQHQRP
                     VMKPKKEVPHYTHAAAVIIGKAGNTRNKILLAQRPESGLLAGMWEFPNGRVSGDAAKG
                     LTKVLKTGYNIKGRAMRNKEALTTVQHAYSHFKVTVHAFQCELQSAPPPGNLRWIPMK
                     KLDEYPMGKIDRQIAKMIAKIQPI"
     gene            4066..4944
                     /locus_tag="VFQ23_04595"
     CDS             4066..4944
                     /locus_tag="VFQ23_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905889.1"
                     /translation="MTERKSVIDPTIIAALIGVCGTVTVALISIFAPRLVPQPTGPTD
                     VPTWTPVVIFTPTITNTPVPTDTVPAGDPTSTAAPDTPTPEPTFTTAPPTPPAIGADW
                     INGCISALWVPWPSTVQTTEVNGCLSEPVNLFFAADGRLTFLANGRFDDTVVYGMFAP
                     VSANGTVSIKSFLTNLQDGEIWMGVFAEPNIESQGMIIVIPPGNVKERPLVQKTMPGQ
                     VELQSTQAFLQDPPIYDVVFDINNGAVSTVLLRDTVFNALPISSAQPWFFVGYQVKRG
                     NNRIDAAFLDLVVQPR"
     gene            complement(5008..6651)
                     /locus_tag="VFQ23_04600"
     CDS             complement(5008..6651)
                     /locus_tag="VFQ23_04600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET9905890.1"
                     /translation="MSKHGKLKIIPLGGLGEVGKNMTAYEFQNQILVVDAGIMFPKND
                     MLGIDYIIPDYQYLRGKADKVVGIVITHGHEDHIGAIHHLLNDIPVPVYATPLTLGLI
                     EGKLARNNALGKARLTKVEAGGSVEIGPFKVEFFHVCHSIPDAVALGITTPAGLVVHM
                     SDFKFDHTPVDGWPTDYAKLADFSRRGVDLLLSDSTNSERPGWTPSEIVIGPAFDKVF
                     TEAKGRVIVATFASLISRVQQVADATAKHGRKMALAGPSMIDNVKIARKMGYLDIPDE
                     LLVPIDQALKMQDHKVVIMCTGSQGEPSSIVGRLSAGTNRQFDLKTGDTVVLSSHPIP
                     GNEETISQTINRLLRRGAEVIYDAVAPVHVSGHASQEEQKLLFNLVKPKHFIPIHGEL
                     RQLKRHAKLAVEVGISEDCVMVVENGQIVELSGGRMRLGERIPGGYVFVDGESIGEID
                     FDIMRERGKLARNGLFLIDISVDKLTGRLLHEPEIITRGFVSPEDAEELLPEVRKHIM
                     DVVNNGGWESEKDIANAARSYLYEETKRRPMVLVTLSKG"
     gene            complement(6861..7496)
                     /locus_tag="VFQ23_04605"
     CDS             complement(6861..7496)
                     /locus_tag="VFQ23_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905891.1"
                     /translation="MMGNKLCPKIILEGTRLTFKTEIAFELNEHPRIVGPRKYRYHSP
                     LISAEWCTFTNYPWGRGPINFEPQEEAQAMETYATWARMFELQKYYSWIVDRFHISTQ
                     AYQKQTYGKNYDFHWLEERLLPLNFRIVFCTRTPESFEAAREERLKVSGNPSQYKDLT
                     PFIREQELMRQLISKSMLPGLTLDISDNNIPVALERIADWMEQSDGLYMPD"
     gene            7764..8333
                     /locus_tag="VFQ23_04610"
     CDS             7764..8333
                     /locus_tag="VFQ23_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905892.1"
                     /translation="MSTISQTPKMNTARTLNINNVNWFIVAALLVTVIAIFNAVARSS
                     TAKTTDHSAYVVYRQGEWLSVPISNEVAYQIFRRREVASPVTTAKAYQMFRKSEVVSA
                     PHLTDAEAYQIFRLGEWAPVSLPAVDLSAYRLSERTLINPTAGLETFLQNERTYVDPQ
                     ASLATYLASERTSTPVQFTKFQLSEWFGQ"
BASE COUNT         2274 a         2067 c         2072 g         2146 t
ORIGIN      
        1 ttacgatcca gacccccacg agaataacgg cacccccgac cagtgacacc cacgtaattg
       61 cctcgtccaa cagaaaaaac gccacaacta ccgccacaag aggttcaata taaagaaaca
      121 ccccggtttc tgcagtcgaa agcgcctgta aagcatcgta ccaggcgata taagctaagc
      181 cggagcaaaa tactcccagg aaagttatgc cgatccagcc attgaaagag agatttggta
      241 tttcaagata attcctgcct gcaatgaatg ccagcgaggt aaagaaccag ccaaacgtca
      301 taacgtaaaa aatcatcagg gtggctgaat aggtttttaa accacgtcgc gacaaggcgg
      361 agaaaacggc ccaattgaag gcgctggcca ggataagaat atctcctggc gccccgaact
      421 cgccaactga aatagatgcc agattcccat ctgtgacaac gagcagcaca ccgaaagccg
      481 ccaggatgat tccaaaaatt tttagtagcg gtaacccctc tcgtaaaatt acccaaccaa
      541 gcagtgccat gaacaccggt gtggtcgcaa cgatccaggc ggtggtgctg gcttcggagg
      601 tttgaagtcc attggattgg agccattgat gaaacgtaat gcctagaaaa ccaagcagtg
      661 cgaaatatcc caattcgctt ttcttcggca gggcaaactg acttctgatt gccactgcaa
      721 tgcctaaaat cacaacgccc attgtaaacc gcagccacac gattgaaatt ggagaaacat
      781 cctgtaaagc aattttggtg gcaatgaacg aagcgcccca ggcgatgacc gccaacagca
      841 cttcaagata aggaacgagc ttggaattgg gcatcaagtt tcctctggaa ttggaatggt
      901 gaattataaa tccgtgaaat cgtacgagga tgtattttag ccaatcactt tgacttgctc
      961 tgctgtttgt tgtaaacttc tcgtgctact tacattgcag aaggtgagag gagcctggaa
     1021 atggaaagtt cagatgtgtc atacgcttct gaaaagttga ctcaactaaa gtcagaatat
     1081 catgtagatg ttattgcgga ttggggaatg ggctcggatg gaaaatggca taacggcaca
     1141 tggtctaaag aggaactcga tcgactacat cgatcaatta gtttgctgac agggatcatg
     1201 aaaggggccg accatttcgt ggaaaacctt ggtggcgtta cagtagtgaa agaggacatc
     1261 ggactacacg gcgggcaagc gcaggcgcat ctcgtcaaat tcaattccaa atcctcattt
     1321 tctgcctgga ccgttgtcca tgagtttgct cacgcctggg atgcaaactt tgactggaag
     1381 ctttccgtcg ctttggaaag gtacacgggt gggtataccc gtccctggtt agcctggttc
     1441 aaaaaactga tcgggatgtc agattcaagg tttttcaaac acgagaacaa acctggcaag
     1501 cgcggcagaa agccgggctg taacgctgca ggttattttt atggagataa acccagcggc
     1561 tccaattgga tgtttaaccg gctcgaagat tttgcagaat ccgtggcaat gtatattggc
     1621 tgggccagga gtaacgatct ttccaactgg gcagaagccc gaataaaacg ttatctgctg
     1681 gaagatggag tcaaagataa gacctttggt acagattact ggaagtatta ttcaaagttc
     1741 ttttacccgg atcgcggcga ttacaccaag accaaacgct ggaagtttgt ggaagaactt
     1801 gtaaaaggaa gaataaagat tgcttaaata taaaatctgc tccaacgaac agcgggtata
     1861 atactcaatt ggaggctaca ctttatggaa attacctggt acggacattc atgttttcgt
     1921 cttaccgagc gcaattatgc aacggtggtg acggatccat atgacaacaa atcaatcggt
     1981 tattctgcat tgaggcttaa atctgatatc gtgacggtaa gtcacgatgc acctggtcac
     2041 aataatacgg atgcagtaaa gggcatatcg catgtgattg atggggctgg agaatttgag
     2101 attggcggtg tgttcatcac cggcgttcaa acagatggtg gcggatcgag caggaagaaa
     2161 agcaaagata aagaggacgg accccggaac accatctatg tatttgatta tgatggcatt
     2221 acagtggcgc atttgggtga tctaaagcaa acaccgactc aatctgagat agagtcgttg
     2281 ggcaccatca atgttgcttt gatcccagtc ggaggtgggg gtgggctgaa tgccgcgaaa
     2341 gccgcggaag tcatcagcct gatcgaaccg aatctcgtca tccccatgca ctattccaca
     2401 ccagatgtca aactctcgct tgactccctg aataaattca tcaaggaaat gggtttgtcg
     2461 acaccggata gtcagccgtc gctgaaagta actcgcagcg gtcttcccaa cgaaaccaaa
     2521 gtcgttgtgc tggattatca atcgagttaa tgacatggct gtaaaagtat tgatgacgtg
     2581 ggatatcgct ggcgaacgcg accaggatta tttcgaattc atcatcggtg agttcatccc
     2641 aggcgtgcaa cggctcggac tccagcccgc agaggcctgg gcaacaattt atggctcgta
     2701 tccgcagata caggtgggtt tgctggcaac cgatgtttca caggcgcgtc agattctcga
     2761 ctcaagtgac tggggtgtgc tgcaggatcg cctcttcaac tatgtaaaaa atttctccta
     2821 taaagttgtg cccgcgcgcg gtgggtttca attctgagtc tgaacacgaa ccccgtttct
     2881 gactcacatt acaaatgaga ttaattcagc gcgcctctta aacatgtctc ggctttcttc
     2941 tcgacttcta gactggtatc acaagaataa gcggactatg ccctggcgtg ttctgcaacc
     3001 tgatccttat cgggtttggg tttccgagat catgcttcag caaacgcgcg tggaaacggt
     3061 cattccatat ttcgagaagt ggatgcgttt gtttccgacc attcaggctt tggcaactgc
     3121 cagtgaacac gatgtgctca atgcgtggga agggcttggc tattacagtc gcgcccgaaa
     3181 tttgcataaa gctgcaaaga ttgttgcaga aaaatataag gggaaattgc caagtgatgc
     3241 ggatgcactg cgtaatttac ccggcatcgg aagatatacc ctcggcgcga tttcttcgat
     3301 tgcatttggt ttggatgtac ctgcgttaga cggaaatatt aaacgggtct atgctcggct
     3361 gtttgatgtg gttgacccag ttgattcgac ggttggtgaa aaaatccttt gggacctggc
     3421 agaaactcat ttgccagaag gccaagctgg cgattacaac caggcactga tggatttggg
     3481 tgcgacaatc tgcctgccaa agaacccgcg ctgtttgatc tgcccattaa tgaaagtatg
     3541 caaagcgcga gtcaacggaa ctcagcacca acgcccggtc atgaaaccaa aaaaggaagt
     3601 gccgcattat acccatgctg ccgcggtcat cattggtaag gccggtaaca cgcgtaataa
     3661 gattctcctg gcacaacgcc ctgaaagcgg cttgctggcg gggatgtggg aattcccgaa
     3721 tgggcgtgtt agtggtgatg ccgccaaagg attgacaaag gtcttaaaga ccggatacaa
     3781 cattaaaggt cgggcgatgc ggaacaaaga agctcttacc accgttcaac acgcatactc
     3841 gcatttcaag gtgaccgtgc atgcgtttca gtgtgagtta caatcggctc ctccacctgg
     3901 aaacctgcga tggattccga tgaaaaaact ggatgaatat ccgatgggca aaatcgatcg
     3961 tcagatcgcg aaaatgatcg ccaaaataca gcctatttaa tgtccttgag aacacatcga
     4021 atatagcgta gaattaggaa aattttttac tgaattggga tacatatgac tgaaagaaaa
     4081 tctgtaattg acccgacgat tatcgcggca ttaattggtg tttgtggcac cgtgacggtg
     4141 gccctcatct ccatctttgc cccaagactt gttcctcaac cgaccgggcc aaccgatgtt
     4201 ccaacctgga cgccggtcgt tattttcacg cctactatta ccaatacacc cgttccaacg
     4261 gataccgtgc cggctggtga cccaacttct actgccgctc cagacacgcc gacacctgaa
     4321 ccgaccttta caacggcacc tcctacgcct cccgcgattg gcgccgattg gatcaatgga
     4381 tgtattagcg ctttatgggt accctggccc agtacggttc agacgaccga ggtcaatgga
     4441 tgtctttcgg aaccagtaaa tctattcttt gccgcggatg gacgactcac cttcctggcc
     4501 aatggcaggt tcgatgacac agtagtgtat gggatgtttg cacctgtttc cgcaaacggg
     4561 actgtcagta ttaaatcatt cctcaccaat cttcaggatg gcgagatttg gatgggtgtt
     4621 tttgccgagc ccaacattga gtcgcaggga atgatcatcg taattccgcc tggtaatgtc
     4681 aaagagcgcc ctctggttca aaagaccatg cctgggcaag tggagctgca aagtacgcaa
     4741 gcgtttttac aggacccacc catctacgat gtagtgttcg atattaataa tggtgctgta
     4801 tcaaccgtct tactgcgtga tacagtcttc aatgccttgc cgatctcttc tgcccaacca
     4861 tggttttttg ttggatatca ggtcaaacga ggaaataacc ggattgatgc agcatttctg
     4921 gatttagtcg tccagccgcg ataatttcac cgatcaagac aacagcccga ccacacctgg
     4981 tcgggctgtt gatttatacg gatgacttta tcccttactt aaggtcacga ggaccatggg
     5041 tcttcgcttc gtctcttcat ataaataaga tcgggctgcg tttgcaatgt ctttttcgct
     5101 ttcccacccg ccgttgttga caacatccat gatgtgttta cgcacttcgg gcaataactc
     5161 ctctgcatct tcaggagaga caaagccacg cgtaatgatt tctggctcat gcagaaggcg
     5221 accggttagt ttatccacac tgatgtcaat cagaaagagt ccattacggg cgagttttcc
     5281 tcgctcacgc atgatgtcga agtcgatctc gccgatggat tctccgtcta caaatacgta
     5341 gccgccgggg atgcgctcgc ccaatctcat ccttccgcct gagagttcca cgatctgtcc
     5401 gttttcaacg accatcacac aatcttcaga gatacctact tctaccgcga gttttgcatg
     5461 tcgctttagc tggcgtagtt ctccgtgaat tggaatgaag tgcttcggct tgacaaggtt
     5521 gaacaaaagt ttttgctctt cctgcgaggc atgacctgag acgtgcacag gggcaacggc
     5581 atcatagatc acttcggcgc cgcgccgcaa gagacggttg atcgtctggc tgatggtttc
     5641 ttcatttcct ggaatggggt gagaagaaag cacgacggta tcccctgtct tgaggtcgaa
     5701 ctgacggttt gtcccggcag ataggcgtcc cacaatcgaa gaaggttcgc cctgggagcc
     5761 tgtgcacatg atgaccactt tgtgatcctg catcttcaac gcctgatcaa tcggcaccaa
     5821 tagctcatcg ggaatgtcca gataacccat cttgcgggcg atctttacat tgtcgatcat
     5881 actcggacct gccaaagcca tcttacgccc atgcttggcg gtagcatccg ctacctgctg
     5941 aacacgagaa atcaatgacg caaacgtagc gacgatgact cgtcccttgg cttcggtgaa
     6001 taccttatcg aaggctggtc cgatcacaat ctctgaaggt gtccaaccgg gtcgttccga
     6061 gttcgttgaa tcagaaagta acaggtctac accgcgccgc gaaaaatccg caagctttgc
     6121 atagtctgta ggccagccat cgaccggcgt gtgatcgaat ttgaaatcac tcatgtggac
     6181 gaccaaccct gccggcgttg tgatccctaa ggccacagca tccgggatgg aatggcagac
     6241 gtggaaaaat tccaccttga acggtccgat ttcgacagag ccgcccgcct ccactttggt
     6301 tagcctggct ttgcctaagg cattgttacg ggctagtttt ccctcgatca gcccgagagt
     6361 caaaggcgtg gcatacaccg ggactggaat atcgtttaaa agatgatgga tggccccgat
     6421 gtgatcttcg tgtccgtggg taataacaat tcccacaact ttatcggcct tcccccgcaa
     6481 atattggtag tcaggaatta tgtaatcgat cccgagcata tcgttctttg gaaacattat
     6541 tcccgcatct acgaccagaa tctggttttg gaattcgtag gcggtcatgt tctttccaac
     6601 ttccccaagt ccacccaggg gaataatctt caattttccg tgcttactca tacaagtatt
     6661 ttatcctcac ttgtttttaa aattatttat ccgccattag cggttaagga cttaaaaaaa
     6721 taagcccccg ctgacttgct cgcgaggctt cgtaccacac atgcgattgt tttcaaacaa
     6781 actgccaagt ggcagtaaaa gtcaacttca acagcttaaa gtatagcagg ggaggagaga
     6841 attgtcaaag aggttgagag ttaatccggc atatacagtc catcggattg ttccatccaa
     6901 tctgcaattc gctctagcgc cacagggata ttattgtcag aaatatccaa tgtcaaacca
     6961 ggcagcatgg attttgaaat taactgacgc ataagttcct gctcacggat gaacggagtc
     7021 agatctttgt attgcgatgg gttgcctgaa accttcaatc tctcttcgcg tgcagcttca
     7081 aagctttctg gcgtccgtgt acagaaaaca atacggaagt tcagcggcag gagtctttct
     7141 tccaaccagt gaaagtcata attcttgcca taggtctgtt tttgataggc ttgcgtcgaa
     7201 atatgaaagc ggtcaacaat ccacgaatag tatttttgca gctcaaacat ccttgcccag
     7261 gtggcgtacg tttccatggc ttgcgcttct tcctggggtt caaaattgat cggtccgcgt
     7321 ccccagggat aattcgtaaa cgtacaccac tctgcggaaa tcagcggcga gtgataacga
     7381 tatttgcgcg gcccaacgat gcgtggatgc tcatttagct cgaaggcaat ttctgttttg
     7441 aaggtcaggc gtgtgccttc caggatgatt tttggacaga gtttgtttcc catcattccc
     7501 agtatagttg attgtattta gtttctcaaa tggttttacc gctggaccag gccatcctat
     7561 tcccactaga gaaataattg agatgtaagg cgtcgtttac attccaccac ccacttgacg
     7621 gtggaaattt gtgttgttta aggaaaacat gcaaaaaaac acgcgcaatc cacgcggaaa
     7681 tacacgctgg aggacggtgg ctggctaaac tggtgtcata gattggcaac caaaacaaac
     7741 tccagccaat ccaggagaat aaaatgtcca ccatttcgca aacccccaaa atgaatactg
     7801 cccgcacttt gaatatcaat aatgtcaact ggtttatcgt tgctgccctt ctcgtaacag
     7861 tgattgccat tttcaatgcc gttgcccgtt catcgaccgc caagacgacc gatcacagtg
     7921 cttacgtggt ttatcgacag ggtgagtggc tttcggttcc cattagcaat gaggtagcct
     7981 accagatctt ccgccgtagg gaggttgctt cgccggtgac tactgctaag gcttatcaga
     8041 tgttccgcaa gagtgaagta gtgtctgctc cccacctgac agacgccgaa gcgtatcaaa
     8101 tcttccgact tggcgaatgg gcacccgtca gtttgccagc cgttgacctg tctgcttatc
     8161 gcctcagtga gcgaaccctg atcaatccaa ctgcaggact ggaaacgttc ctacagaacg
     8221 agcgcactta tgtggatcca caggcaagcc tggctactta tcttgccagc gaacgaacca
     8281 gtacacctgt gcagttcacg aaattccaac tgagtgagtg gttcggtcaa taaaagatgc
     8341 acggagatac tcctccgaaa aagtaagtcc gagcatccgg tacgaggtca gcggcgggaa
     8401 caagcaccgt cgctgatcgt ctttaatcac ttgcaactta attccgagta agtgtatact
     8461 tcgtttgact acaaatcaaa gggagaatat gcttgaagtc agattgcttg gcaagtttga
     8521 aatagttcaa aatgacaacg ccgtcataat ttcttcacg
//
LOCUS       DASTJP010000150         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1188310, whole genome shotgun sequence.
ACCESSION   DASTJP010000150 DASTJP010000000
VERSION     DASTJP010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188310"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2138
                     /locus_tag="VFQ23_04615"
     CDS             <1..2138
                     /locus_tag="VFQ23_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HET9905893.1"
                     /translation="NKAVYLMVDGGTFNGKTVSALGWTKTAAIYYEVNTNLLSSGADY
                     SDLYFALQQACSNMIGQSGITTADCIEVKDAIDAVEMNSQPAPNFNPGAPMCDAGISP
                     TYTFTDGLEGGTSNWTFNNGAHTRWQLDSAYYGPFAHSGLHSLYADDYPPVITDASAR
                     LASFIVPVNGYMHFSHAYEFENYIPSPGPYYFDGGVLEYSTNSGATWLDASALIDFNG
                     YKGVVFTGAGNPLSGRRAFVGTSHGYTSTRLNLTGLVGQTVAFRWRMGLDEGTYFGGW
                     WVDDIKVYSCVHPNHNIDVTVGSLLTGRYYLPVGSSMRQGFSNVDSGPVKIESTNSAN
                     ILSALRVIWKEPGVRFSYSEMLGHPKEQLSTEYWFPWYNNAVPASMDQGFRIANVNAT
                     GTNTIQVSVGSTVIDTLSLGVGASVRVSYSVDNGPIRIACTTCTGSEKIIAALRIIWK
                     EPGYRASYSEMMGLPVEQLSKEYWFPWYNNAVPASMDQGFRIANVDTTASNTVEVWVG
                     STQLDTIVLGAGASTRVSYSVDNGPIRITCSSCTGIGNDKIIAALRVIWKEPGFRASY
                     SEMMGLPAEQLSSQYWFPWYNNAVPASMDQGFRIANISTTESNTVEVWVGSTKLATLT
                     LGAGGSTRVGYNVDNGPIRIVCTTCTNTGSDKILAALRVIWKEPGFRSSYSEMMGLPH
                     EALSTEYWFPWYNFAAPNSMDQGFRIAVP"
     gene            2314..>3926
                     /locus_tag="VFQ23_04620"
     CDS             2314..>3926
                     /locus_tag="VFQ23_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HET9905894.1"
                     /translation="MSTILRLSRLLFVTIIISSIGLSVIQPRAVSAQVSDGIKRQINA
                     ESGKISFIGPESGRAVPAAQALGIFARPADPAVALARRFAPQFGLNDPVRNLSVKQTE
                     TLEGGRITARFQQSYQGIPVIGAELMIHTNGNGDLISINGEVSPNVSLQTQPSIESEQ
                     AMQTALQAIAKWYKKSTAEFTVTSPELWIYDESLLHSSNRPVELVWRMEVISLENSLA
                     VHELVLVNAHRGQISLHFNQIDTAWHSDATKLANGPHSTRRETSSENTALLFSALVNT
                     HTANSTSSLPGTLLCNQSQPNCTNGSNLNADSAHKYAMGTFNLYDSKHNRNSINNGGM
                     TIISTVEYCEGFDCPYGNAFWSGTQMVYGNLFGFARADDVVGHELTHGVTQYESNLFY
                     YYQSGAINESFSDLWGEYYDQTNSQGTDTAPVKWQIGEDVTGYGAFRNMNDPAAFGDP
                     DRMSSPNYHTTDDDNGGVHHNSALNNKAVYLMVDGGSFNGKTVTALGWDKTAAIYYEA
                     QTNLLLSGSDYSDLFYALQQACSNLIGQKG"
BASE COUNT          982 a          979 c          978 g          987 t
ORIGIN      
        1 ataacaaggc tgtctacctg atggtcgatg gaggcacgtt caacggcaaa actgtatctg
       61 ctttaggttg gaccaagacc gccgccattt attacgaggt gaataccaac ctgctttcct
      121 ccggtgcgga ttactccgat ctctattttg ccctccagca agcttgttcg aacatgatcg
      181 gtcagagcgg aatcacaacc gcagactgta tcgaagtcaa agatgctatt gacgcagtcg
      241 aaatgaactc tcagcctgca ccaaatttca accccggtgc gccaatgtgt gatgcaggca
      301 tttcacccac ttatacattt acggatgggc tcgaaggtgg cacttcaaac tggacattca
      361 acaacggagc ccacacacgg tggcaacttg attcggctta ttatggacca ttcgcgcact
      421 ctggtctgca ctctctatat gcggatgatt atccacctgt catcaccgac gcgagcgcca
      481 gactagcttc gtttatcgtc ccggtgaatg gctatatgca cttttcacac gcgtatgagt
      541 ttgaaaatta tattccatcc cctggcccgt attactttga tggtggtgtt ttggaatata
      601 gtactaacag tggtgcgacc tggctggatg ctagtgcatt gatcgatttt aatggctata
      661 aaggtgtcgt ctttacgggc gcgggaaatc ccctttcggg ccgtcgggca tttgttggaa
      721 ccagtcatgg gtataccagc actcgcctca accttactgg gcttgttgga caaaccgtcg
      781 ccttccgttg gagaatggga ctcgacgagg ggacctattt cggtggatgg tgggtggacg
      841 atatcaaggt gtattcctgc gttcatccca accataatat tgatgtcaca gtaggcagtc
      901 tattaacagg tcgatattat ctacccgtag gaagtagcat gcgccagggc ttctcgaatg
      961 tagacagtgg accagtaaaa attgaaagca ctaacagtgc aaatatcctc tcggccctgc
     1021 gcgtgatctg gaaagagccc ggggtgcgct tcagttattc cgagatgctc ggccatccca
     1081 aagaacaact ttctactgaa tattggttcc cgtggtacaa caacgcagtg cctgcttcca
     1141 tggaccaggg cttccgcatc gctaacgtca atgcgacagg aacaaataca atccaagtct
     1201 cagtcggcag cacagtgatt gacacgttaa gtcttggtgt tggggcgagt gtcagagtaa
     1261 gctactcagt tgataacggc cctattcgaa ttgcctgcac cacctgcacc ggcagtgaaa
     1321 aaattatcgc ggctttgcga atcatctgga aggagcccgg ctatcgagca agttactccg
     1381 agatgatggg actgccagtc gaacagcttt ccaaggaata ctggttcccc tggtacaaca
     1441 acgctgtccc cgcttctatg gaccagggct tccggattgc caatgtggat accacagcta
     1501 gcaacacggt cgaggtctgg gtgggatcta cgcagctcga tacaattgtc ctgggcgccg
     1561 gagcgagtac tcgcgtaagc tattcggtgg acaatggtcc aattcgaatt acctgtagca
     1621 gctgcacagg aattggcaac gataagatca tcgctgcact gcgggtgatc tggaaggaac
     1681 ctggcttccg cgccagttac tctgaaatga tgggtctgcc agccgaacaa ctttccagcc
     1741 aatactggtt cccgtggtac aacaacgctg tgccggcatc catggatcag ggattccgga
     1801 tcgcaaacat ctccaccacg gaatcgaaca cagtcgaggt ttgggtgggt agtaccaaac
     1861 tggcaactct tacgcttggg gctggaggaa gcacacgcgt aggctacaat gtggacaatg
     1921 gtccgattcg tattgtctgc acgacatgta ccaacaccgg aagtgacaag atcctggccg
     1981 cgctgcgggt gatctggaag gaaccggggt tccggtccag ctactctgaa atgatgggtt
     2041 tgccccacga ggcactttcc accgaatatt ggttcccctg gtacaacttt gcagctccca
     2101 actccatgga tcagggtttc agaatcgccg taccgtagaa cgcagtttac aggaatgaag
     2161 tcaccggcaa ttaactgcgt tttgccggtg acttttttat gacaatagcc gtatttttta
     2221 tggtaacgta tttattgatc ggtcgtcttg aattcgatgc tatttgtttc aaagtggtta
     2281 ataactaatt tgcgttccca atgaggagtt ttcatgtcga caattctccg gctgtcccgc
     2341 ctgcttttcg taaccatcat aatttcttct atcggcctct ctgtgatcca gccgagggcg
     2401 gtttccgcgc aagtttcaga tggcatcaaa cgacagataa atgctgagag cggcaaaatc
     2461 agtttcatcg gtccggagag tggtcgcgcc gttccagctg ctcaggccct gggaattttc
     2521 gctcgtcctg ctgacccagc cgtggctctg gcgagacgat ttgcacccca atttggactg
     2581 aatgatccgg tccgcaacct atcggtgaaa caaaccgaaa cgctcgaagg tggaagaatc
     2641 acagcgcgtt ttcaacaaag ctatcagggc atcccggtaa tcggtgcaga gctgatgatc
     2701 catactaatg ggaacggtga tttgatttca atcaacggtg aagtatcccc aaacgtttct
     2761 ctacaaactc agccttccat tgaatcggaa caagcaatgc agaccgccct tcaagccatc
     2821 gcaaagtggt acaaaaaatc tacagcagag ttcacggtca catcgccgga attgtggatc
     2881 tacgacgaaa gcttattaca ttccagcaac cgaccggtcg agttggtttg gcgtatggag
     2941 gtgatttcgc tggaaaacag cctggcggtt catgaattgg tgcttgtaaa tgcgcatcga
     3001 ggtcagatca gtctacactt caatcagatc gacacagcct ggcactctga tgctactaaa
     3061 cttgcaaatg gacctcattc caccaggcgt gagacgtcgt ctgagaacac ggccttgttg
     3121 ttttccgccc tggtcaatac acatactgcg aatagtacat catcccttcc tggtaccctc
     3181 ctctgtaacc agtcccagcc aaattgtaca aatggaagca atttgaatgc cgatagtgct
     3241 cacaaatacg cgatgggtac ttttaacttg tatgattcca agcataaccg gaacagtatt
     3301 aacaatggcg gaatgacgat catatccact gtagaatact gtgaaggttt tgactgtcct
     3361 tatgggaacg cgttctggag cgggacccaa atggtttatg gtaatctctt cggctttgcc
     3421 cgagctgacg atgtggtggg tcacgagctc acgcatggtg taacgcaata tgagtcgaac
     3481 ttgttttatt attatcagtc gggcgccatc aatgaatctt tttcagactt gtggggcgaa
     3541 tactacgatc aaaccaatag tcaaggtaca gataccgcgc cggtaaagtg gcagataggt
     3601 gaggatgtca cgggttacgg cgcattccga aatatgaatg atcccgctgc gtttggcgat
     3661 ccggatcgga tgtccagccc caactatcat acaactgacg atgataatgg tggtgttcac
     3721 cataatagcg ctctcaataa taaagctgtt tatcttatgg tggacggcgg ctcattcaat
     3781 ggcaagacag taacagccct tggctgggat aaaacagcag ccatctatta cgaagcgcag
     3841 acgaatctcc tcctttccgg ttctgattac tcagacttgt tttacgcgct ccagcaggcc
     3901 tgctcaaacc tgatcggaca aaaagg
//
LOCUS       DASTJP010000151         4779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1299792, whole genome shotgun sequence.
ACCESSION   DASTJP010000151 DASTJP010000000
VERSION     DASTJP010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299792"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VFQ23_04625"
     CDS             <1..1024
                     /locus_tag="VFQ23_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9905895.1"
                     /translation="IIVGTSIGAVNGAAIAQGMSADRLVEVWKSLEEKDIQGLPPGMR
                     GIARWVAGTIFKEVMDTKLPQIHPKEATSPAPKEFWPPLPLLPNWIAERLVGRWINLL
                     DTGPLRKTLYARFNLDEARLAASEKSLLIAATKVQTGERVMFSNREVMDHKRRKMRQD
                     VFTGITADRILASCSIPLVYPWTFDAETDAYYWDGALVANTPLGAAIDMMGGDTNIPA
                     EVVVVMMTPWWESGEPAPAKVHSEPKSFGDAITWMLDWMLLASFRENLKMIRAFNEMA
                     LRERKEGPPPYRYRLVTPIIVSPMEFLRAERIIDYDGDVSAKLIDEGYRAAKYAFEKA
                     FPVSQS"
     gene            complement(1041..2084)
                     /locus_tag="VFQ23_04630"
     CDS             complement(1041..2084)
                     /locus_tag="VFQ23_04630"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HET9905896.1"
                     /translation="MIKPRKLQPGDTVAAISLSWGGPGTFSHRYEAGKQQLQDEFGLK
                     VVETAHALRDADWLHKNPKARAEDLTNAFADPSIKAIISTIGGDDSIRILPYLDLDVI
                     RSNPKIFMGYSDTTITHIACFNAGLVSFYGPAVMTGFAENGGMFPFMVEAVRRSLFSS
                     APIGEVQPHTGGWTVERLDWGNPENQTRKRSLTLSNGWKFLQGRGICQGHLIGGCLEV
                     LDFLRGTDYWPQANAWKNAILFLETSEEAPPPSVLKCSLRSYASMGILKQLSGILFGR
                     PGGEILPDRFHEYDEVLQQVVTHEEGLFELPIITHMDFGHTDPMFVLPYGVNAEINCE
                     SQEFSILENAVVD"
     gene            complement(2189..2926)
                     /locus_tag="VFQ23_04635"
     CDS             complement(2189..2926)
                     /locus_tag="VFQ23_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9905897.1"
                     /translation="MIKRIVQFAVVLVILVTSFGSIRGASAWSCPSYITVQWGDTLSG
                     IAVQCGTTVYAIQRANPGLGWWVYAGQVLYIPTGYGDVPVSYPPSGGTYVVQKGDTLG
                     KLAARLGIRLSDLLAVNPQINNASLIYVGQVITLPAGVSVPPPVYNPPPSQPHYPVPP
                     VNSYSSVKVNYKYGLYVRSAPGGDIIASALNGTTWRYNQGSLFFDNKWKLWVEVKLIP
                     PVKGYTTGWILVKDQFGTYFTDPPIDE"
     gene            complement(2998..>4779)
                     /gene="pepF"
                     /locus_tag="VFQ23_04640"
     CDS             complement(2998..>4779)
                     /gene="pepF"
                     /locus_tag="VFQ23_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET9905898.1"
                     /translation="VKKNQTWNSESVFASPSAFDAEVDSLIQSLQDLEKFRGHLGENI
                     DTFIEAMDTMNVISQRAAKIQVYGSLSSAVDTSNQQGVSMIIKAMSTVAQVSAATSFV
                     DPELLAIGEAKLRQWLKDDLRMTMYEHYFTDLFRRQAHVRSAEVEQILGMLREPFSNT
                     RGTTNLLANADFKFKPARNSKNKKVELTQSTFDAILNGADRKARRTAWENYNDQFLAY
                     KNTLANNLATSIKQNVFNMKVRNFNSSLEATLFDGEVPVEMFDNLLNIFQKNLPIWHR
                     YWKIRRKALGVKTLHPYDVWAPLTTKKHTVPFEKAVEWICEGLAPMGDEYVSVMRKGC
                     LQDRWVDWSPNAGKRQGAFSTRVPKDTYPFILMSYTDDIGSMSTLSHELGHSMHAYYA
                     SRAQPMIYYTYPSIISETASNFNQAMTRAYLLNSNPNKYFQIALLEEAMDNFHRYFFI
                     MPTLARFELETHQRIENGQALTADSMIELMADLFSEGFGGNMNLDRERVGITWGTFTT
                     HLYIDYYSFQYAIGISAANAIAQRVLNGAPGAAEDHFNFLRAGSSQSPMDVFRIAGID
                     MTGTEPIEDAFAVLSSYVDRLETLTAR"
BASE COUNT         1189 a         1228 c         1180 g         1182 t
ORIGIN      
        1 aatcatcgtt ggcacatcca ttggcgctgt caatggtgcc gcgattgctc aggggatgtc
       61 tgccgaccga cttgtggaag tgtggaagtc actggaggag aaagatattc agggcttgcc
      121 gccgggaatg cgtggcatcg cgcgctgggt cgcggggact attttcaaag aggttatgga
      181 cacaaagctt ccgcagattc atccgaagga agctacttca cctgctccca aggaattttg
      241 gccacccctg cctctacttc caaactggat cgctgaacgg ctggtgggtc gctggatcaa
      301 tctactggat actggtccac ttcgcaagac cctctatgcg cgcttcaacc tggatgaagc
      361 gcgattggca gccagtgaaa aatcgttgct catcgctgct accaaagttc aaactggcga
      421 gcgcgtgatg tttagcaatc gcgaagtaat ggatcacaaa cgccgcaaga tgcgacaaga
      481 cgtcttcacc ggaatcaccg ccgatcgaat cctggcttca tgttcgatcc ccctggtcta
      541 cccatggacg tttgatgcag agacagatgc ttattattgg gatggcgcgt tggtggccaa
      601 tacgccgctg ggtgccgcca tcgacatgat gggcggggac acaaatatac ctgcggaagt
      661 tgtcgtggtg atgatgactc cctggtggga aagcggcgag cccgcacctg caaaggtcca
      721 ctcggagccc aagtccttcg gagatgcgat tacatggatg ctcgattgga tgctgctggc
      781 ttccttccgc gaaaacctga aaatgattcg agccttcaat gagatggcgc tccgcgaacg
      841 caaggagggt ccgcctccct atcggtatcg cctggtgaca ccgattatcg tttcaccgat
      901 ggagtttcta cgggccgaac gaatcattga ttatgatggg gatgtctctg ccaaattaat
      961 tgacgaagga tatagagctg cgaaatatgc cttcgagaaa gcctttccag tgtcacagag
     1021 ctaattcatc attgtctatc tcaatcgaca acagcatttt caagaatact gaactcctgg
     1081 gactcgcaat tgatttcagc gttcacccca tatggcaaaa caaacatagg gtcagtgtga
     1141 ccgaaatcca tatgggtgat tatcggaagt tcgaaaagcc cttcctcgtg agttacaacc
     1201 tgctgtagaa cttcatcgta ttcatggaaa cgatctggca aaatttctcc gcccgggcgg
     1261 ccaaacaaga ttcccgagag ttgcttcaaa attcccatcg aagcatagga tcgaagtgaa
     1321 catttcaaca cactgggtgg aggtgcttct tcagacgttt ccaaaaatag aattgcattc
     1381 ttccaggcat tcgcctgggg ccaataatct gtaccccgca aaaaatcgag cacctcaagg
     1441 caaccgccga tcaaatggcc ctggcagatt cccctccctt gaagaaattt ccatccgttg
     1501 gatagagtca ggctgcgctt ccgggtttga ttctcagggt ttccccaatc cagcctttcc
     1561 acggtccaac caccggtatg gggttgaacc tctccgattg gcgcagatga aaaaagtgat
     1621 ctacgcactg cctcaaccat gaagggaaac atgccgccat tctcggcaaa gccagtcatg
     1681 accgccggtc cataaaatga aaccaggccc gcattgaaac aggcgatatg ggtaatggtc
     1741 gtatcagaat accccataaa aattttggga ttggagcgta tcacatcgag atcgagataa
     1801 ggcaaaattc gaatcgagtc atcgccgcca attgtggaga tgatggcttt gatcgaaggg
     1861 tctgcaaagg cgttcgtcaa gtcttccgct ctggctttcg ggtttttatg aagccagtcc
     1921 gcgtctcgca acgcatgagc tgtttcaaca accttgagcc caaattcatc ttgtaactgt
     1981 tgtttgcctg cctcataacg atgggaaaag gttccgggtc ctccccagga gagactaatc
     2041 gctgcgactg tatctccggg ctgaagtttc cgaggcttga tcatagttat cgttttcctt
     2101 cctgcaacac tttaaaaatt atatctatcc cactttacgc aacgaaaaaa gccctgagtt
     2161 agctcagggc tttttgcaaa tatcttgttt attcatcaat cggcgggtcg gtgaagtaag
     2221 tcccgaactg atccttcacc aggatccagc ccgtggtgta tcctttaacc ggtgggataa
     2281 gcttgacttc cacccacaat ttccatttat tatcaaaaaa gagggagccc tggttatatc
     2341 tccacgttgt gccattcagt gcagaagcga ttatatctcc acccggcgcg ctgcgaacat
     2401 acagcccata tttataattg accttcacac tggaatagga attcacggga ggaaccggat
     2461 agtgcggctg tgatggcgga ggattgtaca cagggggtgg cacgctgacg cccgccggca
     2521 aggtgatcac ctgcccgacg taaatcagac tggcgttatt gatctgcggg ttcactgcca
     2581 ggaggtcgct caggcgaatg cccagcctcg cagctaattt cccaagcgta tctcccttct
     2641 gcaccacata cgtaccccct gagggcggat aggaaacggg aacatccccg tagcccgttg
     2701 gaatatataa gacctgcccg gcatacaccc accagcccag cccggggttg gcgcgctgaa
     2761 tggcatagac agtggtaccg cactgcacgg cgattccgct taaggtatca ccccactgaa
     2821 ccgtgatgta acttgggcag gaccaggcgg atgccccacg aatcgatcca aaagaggtta
     2881 ccagaattac cagaacgaca gcgaattgga caattctctt tatcattgca ctctcctaac
     2941 taaaaaacgc tgaaattaca ggcaaaatgc acagtcttac accatagtgt tacaacccta
     3001 tcgggcagtc aaagtttcca gccgatccac gtaactgctc agaaccgcaa aggcgtcttc
     3061 gatgggttcg gtgcctgtca tatcgatacc agcgatcctg aaaacatcca tcggcgattg
     3121 agaggaacct gctcggagga aattgaaatg atcctctgca gcacccggcg caccgttcag
     3181 gactcgttgg gcgatcgcgt tggccgccga gatgccgatg gcatattgga acgagtaata
     3241 gtcaatgtac aagtgagtcg tgaatgttcc ccaggtaatc ccgactcgtt cgcggtccaa
     3301 attcatattc ccgccaaagc cttcgctgaa taggtctgcc atcaactcga tcatggaatc
     3361 tgcggtgagg gcctgtccat tctcgatccg ctgatgtgtt tccaactcaa aacgagccag
     3421 agtcggcata atgaagaaat atcggtggaa gttatccatg gcttcttcca gcagcgcaat
     3481 ctgaaaatat ttattgggat tactattcag caaatatgca cgcgtcattg cctgattgaa
     3541 gttggaagcc gtttcagaga taatcgaggg atacgtatag taaatcatgg gctgtgcgcg
     3601 gctggcatag taggcatgca tcgagtgacc cagctcatgc gagagagtac tcatactgcc
     3661 gatgtcatct gtgtagctca tcagaataaa tggatatgtg tccttcggca cgcgggttga
     3721 aaaagcgccc tgtcgtttac ccgcattcgg tgaccaatcc acccagcgat cctgcaggca
     3781 gcctttgcgc atcacactta catattcatc ccccatcggc gccagccctt cgcagatcca
     3841 ttccactgct ttttcaaaag ggaccgtatg ctttttcgtc gtgaggggcg cccatacatc
     3901 atagggatgg agtgtcttca cacccaacgc ctttcggcgt atcttccaat agcgatgcca
     3961 aatcggtaga ttcttttgga aaatattgag caaattatcg aacatctcca ccggcacttc
     4021 tccatcgaaa agtgtagcct cgagggatga gttgaaatta cgcactttca tgttgaacac
     4081 gttctgtttg atcgaggtcg ccaggttatt tgccagggtg ttcttataag caaggaattg
     4141 atcgttatag ttctcccatg ccgttcgacg tgccttccga tcagcaccat taaggatggc
     4201 gtcaaacgtg ctttgagtta actcgacttt tttgttctta ctgtttcgcg cgggcttgaa
     4261 tttgaaatca gcatttgcca gtaaattcgt cgtgccgcgc gtgttgctga acggctcacg
     4321 taacattcca aggatttgtt ccacttcggc gctgcggacg tgcgcctgtc ttcgaaacag
     4381 gtcagtgaaa taatgctcat acatcgtcat gcgcagatcg tccttgagcc attgacgtaa
     4441 tttcgcttca ccgattgcca ggagttcggg gtccacaaaa gatgttgcag cgctcacttg
     4501 ggccaccgtg ctcatggctt taatgatcat gctcacgcct tgttgattag aggtatcgac
     4561 tgcgctggat aaagatccat acacctggat tttggcagcg cgttgagaga tgacgttcat
     4621 cgtatccatc gcttcaatga acgtatcaat attctcgccg aggtgacctc gaaatttttc
     4681 gagatcctga aggctttgaa tgaggctatc aacttcggca tcgaaagcgg aaggcgaggc
     4741 aaaaacactt tcggaattcc aggtctggtt cttttttac
//
LOCUS       DASTJP010000152         4294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_241039, whole genome shotgun sequence.
ACCESSION   DASTJP010000152 DASTJP010000000
VERSION     DASTJP010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241039"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFQ23_04645"
     CDS             complement(<1..378)
                     /locus_tag="VFQ23_04645"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HET9905899.1"
                     /translation="MIRKPFVWVAGLVVLGVLIQFVPYGHDHTNPPVMQEPQWDSMQT
                     RTLAKRACFDCHSNETVWPWYSNIAPASWLIYRDVAEGREHFNFSEWDMHPALPEGEG
                     EGEEHQHGPEVIKEVLESGEMPPK"
     gene            complement(467..877)
                     /locus_tag="VFQ23_04650"
     CDS             complement(467..877)
                     /locus_tag="VFQ23_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905900.1"
                     /translation="MMPRLSVYFIRASLIYLLLGFTFGALILANKGLIIVPSIWNLLP
                     IHIEFDLIGWLVQLAMGVAFWILPRFSKAPIRGNERLSWSAFILINAGILFVVADGLL
                     ETNGLMLIGRVFEALALVLFAVGNWRRIKAHGVG"
     gene            complement(874..1281)
                     /locus_tag="VFQ23_04655"
     CDS             complement(874..1281)
                     /locus_tag="VFQ23_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905901.1"
                     /translation="MPKLTRWFIKAALLYLVLALCSGIFLALPAGSLIAGIFPIYIHT
                     LVFGWLTQLIFGVALWMFPKYSLAKPRGHEWLGWASFILLNLGLVLRIIFEPLHGILS
                     SPLTSWVLVISAILQWLSGVAFVANTWARVREK"
     gene            complement(1284..1517)
                     /locus_tag="VFQ23_04660"
     CDS             complement(1284..1517)
                     /locus_tag="VFQ23_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905902.1"
                     /translation="MIFGHAPIIFPAILGAPIAHTPAFYAHVSLLHLSLVVRIVGDLN
                     GQMDIRLVGGLLNEVAILLFLGLTIYSIVKGKK"
     gene            1503..1664
                     /locus_tag="VFQ23_04665"
     CDS             1503..1664
                     /locus_tag="VFQ23_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905903.1"
                     /translation="MTEDHGHHEANKDSVQCCIIEGTGITRTDDKVEDSTDTKPDESA
                     KEANGDIAG"
     gene            complement(1713..1976)
                     /locus_tag="VFQ23_04670"
     CDS             complement(1713..1976)
                     /locus_tag="VFQ23_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905904.1"
                     /translation="MISGHFTPRLTVFGFPIFKIIFLWMGFLVLTIGGERLELSRVLQ
                     PTVSQRRIFTAITIAVLSGAILSLFTLQWGARLSGLSMLVLAL"
     gene            complement(2102..2890)
                     /locus_tag="VFQ23_04675"
     CDS             complement(2102..2890)
                     /locus_tag="VFQ23_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET9905905.1"
                     /translation="MQTEQLYRTDLTDSQWNLIKEMLPAPKAGGRPRRLDLRQVINAI
                     LYLVVGGIQWRLLPKEYPKWQSVYHYFREWRNNGLWPRIHDTLRARVREKAGRHKHPT
                     AGCLDSQSVETTLVAGVRGYDAGKHVMGRKRHILVDTLGLLLAVLVTAASVSDPAGAR
                     QLLSRLGGFCKNVRKIWADGTYRGALLTWVAVHFRFRIEPVLPPKGQKGFQVLPRRWV
                     VERTFAWCDAHRRLSKDYEGLTDSSEAIIYIAMIRLLLRRLAPH"
     gene            complement(2960..>4294)
                     /locus_tag="VFQ23_04680"
     CDS             complement(2960..>4294)
                     /locus_tag="VFQ23_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905906.1"
                     /translation="AGLIPVINGSRVTVLRALSGGMAEEHGISALKHKDGEKRLPWFD
                     WIQVKTTRLLAKPGIHIPRPFVISLRNTFRRKSRLALTLFTLTMGGAIFIAVFNVRVT
                     LHDYIGQIGKYFVADVSLDFERPYRLTEIKQRVMEIEGVEHAEGWQFVSGEVLDAQGE
                     VLENINIFGPPADSQLIDPTLVAGRWIQADDIRKLAVSEGALKYFPDLKPGDTLNLRI
                     DGRDEVWEVIGIFKFVDREGVLAYAPYEYVSKVNHLVNRAFSFRLVTDRHARPYQDAK
                     AEEVDKYFRDEGFKVRIAEAGRASLDTAVESLDILVVFLLIMAVLTAIVGVMGLTGTM
                     GMNVLERTREIGIMRAIGADDRAVMRTVIAEGVFIGMISFGLAIIVSIPLTYLLSTIV
                     SVAIFQTPIDVVFTSTGYGMWLGVVLVLSALASVLPARNAARLTIREVLAYE"
BASE COUNT         1142 a         1176 c         1052 g          924 t
ORIGIN      
        1 cttgggaggc atttctcctg attccaggac ctccttgatg acttcgggac catgttggtg
       61 ttcttcgccc tctccttctc cttctgggag cgctgggtgc atatcccatt ctgaaaaatt
      121 gaaatgctca cgcccttctg ccacgtcgcg atagatcagc caagaggcag gtgcgatgtt
      181 gctgtaccag ggccaaaccg tttcgttgct gtgacagtca aagcaggcgc gtttggcaag
      241 tgtgcgcgtt tgcatgctat cccattgtgg ttcctgcatc acaggcggat tggtatgatc
      301 atgtccgtaa ggaacaaatt gaatcaacac gcctagcaca accaggccgg ccacccaaac
      361 aaatggttta cgaatcattt tcattatcac ctctaattca cttgggaaag attatatgtc
      421 gctttgattc atcaagggat tgcatttctt caaaaacgaa tatgctttac ccaacgccat
      481 gggctttgat acgcctccaa tttccaaccg cgaacaatac caatgccagg gcttcaaaca
      541 ctctgccaat aagcataagt ccattggttt ccaacaatcc atctgctaca acgaatagga
      601 ttccagcgtt gatcagaatg aatgcagacc agctcagtcg ctcatttccg cgtatggggg
      661 ccttggaaaa gcgcggcaaa atccagaagg caacgcccat agcgagctga accagccagc
      721 ctatcagatc aaattcgata tgaatcggaa gcaggttcca tatcgagggt acgatgatga
      781 gtcccttgtt agcaagaatc aatgcgccaa atgtaaagcc taacaacaga tagatcaatg
      841 atgcccggat gaaataaaca ctgaggcgcg gcatcatttc tcccggacgc gtgcccacgt
      901 attagcgaca aacgccacgc cggataacca ttgcaggatg gcggagatca ccagcaccca
      961 actggtaagc ggcgatgaca ggataccgtg taaaggctca aagatgattc gtaacaccaa
     1021 cccgagattc agaagaataa agctggccca tcccagccat tcatgtcccc gaggcttggc
     1081 gaggctatat tttggaaaca tccataaggc gacaccaaag atcaattgtg tcaaccagcc
     1141 aaacaccaag gtatgaatgt agatcggaaa gatgcctgct atcaggcttc cagcaggtag
     1201 cgccaggaaa atcccggagc ataatgcaag gactaaatag aggagagcgg ctttgatgaa
     1261 ccagcgggtc agtttgggca cgtttacttt ttacccttaa cgatggaata aatcgttaag
     1321 cctaaaaata acaaaatggc gacctcattc aataaaccgc cgaccaggcg aatatccatt
     1381 tggccgttta gatccccaac gatgcgtacc acgagtgaca aatgcaaaag ggacacgtga
     1441 gcataaaagg cgggagtatg tgcaataggc gcgcccaaaa tcgcgggaaa gataatcggc
     1501 gcatgaccga agatcatgga catcacgaag ccaacaaaga tagtgtgcag tgctgcatca
     1561 tagaggggac cggcataact cgcaccgatg ataaggttga ggattccact gataccaagc
     1621 cagatgaatc cgctaaagag gcaaatggcg atatagcggg ttagtggatt ctgatggtgg
     1681 atattgcgcc tggcaagatc atttttgagg aatcaaagag ctaaaacgag catggaaagc
     1741 ccgctcagac gcgcacccca ttgaagtgtg aacagagaga gaattgcacc tgatagcaca
     1801 gcgattgtga tcgcagtaaa gatacgcctc tgtgaaacag ttggctgtag tacccgactc
     1861 agttcgaggc gctccccacc aatcgtcagg accaggaaac ccatccacag gaagatgatc
     1921 ttaaagatgg gaaatccgaa cactgtcaag cgcggggtga aatgtccaga aatcatcggt
     1981 ttaaaatggg gtcttcttca atttatgtga aaccccattt gccttgccag agaactcgtt
     2041 ttcgtaaaag atcatctttc gcgcgaccat acatcagccg ctttagagac tgtttaagaa
     2101 gttagtgtgg agccaaacga cgaagcaaaa ggcgtatcat ggcgatgtag atgatggctt
     2161 cactggagtc cgtcaatcct tcgtagtcct tgcttaggcg gcggtgagcg tcgcaccagg
     2221 caaaggtgcg ttcgaccacc cagcgtctgg gcaaaacttg aaagcctttt tgccctttgg
     2281 gtggcagcac tggttcaatg cggaaacgaa agtgaactgc gacccaagtc agcaacgctc
     2341 cgcgataggt gccatcggcc cagatcttgc gtacattctt gcagaagccg cctaaacggc
     2401 tgaggagttg cctggcaccg gctggatctg acaccgaagc cgcggtaaca agtactgcga
     2461 gcaataatcc aagggtatcc accaaaatat gacgttttcg gcccatgaca tgtttaccgg
     2521 catcgtaccc gcgcacccca gccaccaggg tcgtctcaac actttggctg tcgagacagc
     2581 cagcggtcgg atgcttatgg cgtcccgctt tctctcggac acgcgcccgc aaggtatcat
     2641 gaatgcgtgg ccaaagccca ttgttgcgcc attctcgaaa atagtgatag acactttgcc
     2701 atttcggata ttccttgggc agcaggcgcc attggatccc gcctaccacc aaatacaaaa
     2761 tggcgttgat cacttggcgc agatccagcc tacggggtcg tccacccgct tttggcgcag
     2821 gaagcatttc tttgataaga ttccattgac tgtcggtcaa atcggtccgg tatagttgtt
     2881 cggtctgcat aaccgccaac tctcccacca cattgcccga cagtcaaagc tttttaaaca
     2941 gtttctaagg atttttcgct tactcatacg ccagcacttc acgaatggtc aaccgcgccg
     3001 cattacgggc aggcagaaca ctcgccaggg cggataatac cagcaccacg cccagccaca
     3061 ttccataacc tgtagatgtg aagaccacat caatcggtgt ttgaaatatt gccacactca
     3121 cgatcgtcga aagcaaatac gtcagcggaa tagaaacgat gatcgccaga ccaaaggaaa
     3181 tcatgccaat gaagaccccc tctgcaatca ccgtccgcat gacagcgcgg tcatccgcgc
     3241 caatcgcgcg catgatcccg atttcacgag tacgttccaa cacattcatg cccatggtac
     3301 ccgtcaggcc catcacccct acgatcgccg tcagcaccgc cataatcagc aggaacacga
     3361 ccagaatatc caggctttcc acggcagtat ccaatgaagc ccgccccgcc tcggcaatgc
     3421 gaactttgaa gccctcgtcg cggaaatatt tatccacctc ttctgctttc gcatcctgat
     3481 agggacgtgc atgccggtcg gtgaccaatc ggaaggaaaa ggcccggtta accaggtgat
     3541 tgaccttcga gacatattca tacggggcat aggccagcac cccttcccgg tccacaaact
     3601 tgaagatgcc gatcacctcc caaacttcat cacggccgtc gatccgcagg ttgagggtgt
     3661 cacccggctt gagatccggg aaatatttga gtgcgccttc actgacagca agcttgcgaa
     3721 tatcgtccgc ctggatccag cggcctgcaa ccagggttgg atcgatcaac tggctgtccg
     3781 caggagggcc gaagatgttg atgttttcca gcacttctcc ctgagcatcc agtacctcac
     3841 cgctgacgaa ctgccagcct tccgcatgct ccacaccctc gatctccatc accctttgct
     3901 tgatctcggt caatcgatag ggccgctcaa agtccaggga gacatctgcg acgaagtatt
     3961 tcccgatctg accgatgtag tcgtgcaggg tgacccgcac gttgaagacc gcaataaata
     4021 tggctccacc catcgtgagc gtgaacaggg tcagagcgag gcggctcttc cgacggaagg
     4081 tgttccgcag tgagatcaca aacggacgcg ggatgtggat gccgggcttc gcaagcagac
     4141 gcgtcgtttt gacctggatc cagtcaaacc agggaagccg cttctcccca tctttgtgct
     4201 tcaacgcaga tatgccatgc tcttcggcca tgcccccgct gagtgcacgc aggacggtca
     4261 cacgcgatcc attgatcacc ggaatcaatc ccgc
//
LOCUS       DASTJP010000153         4354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1186986, whole genome shotgun sequence.
ACCESSION   DASTJP010000153 DASTJP010000000
VERSION     DASTJP010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186986"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VFQ23_04685"
     CDS             complement(<1..1481)
                     /locus_tag="VFQ23_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9905907.1"
                     /translation="MGFFSGLNDEKYDRQYTDRELMRRIFEYFKSQTRRLVFVTVLVV
                     VLAGIGAALPVVVARMVDSLNEQPSFQEIALIGLAVLMIGFGLWGLNWARRSLVVRAV
                     GDVVLDLRTRAFRAAAEHDLSFYDQFSSGRIVSRITSDTNDFGQLVVIVTDVGSQVVQ
                     AIILGVVLFRTDLRLSLMLMAFLPIIFLAAGGFRVLARRVTKRGMKAMSDVNAAIKET
                     ISGISIAKNFRQEESIFKSFDESNQQSYQVNVQRGFVLSLVFPVLNALGGIFTAILIY
                     VGGLSAAEGIVTVGAWYLFIMSLDQFFFPVLNLSAFWAQIQAGLSAAERAFALIDADP
                     NVVQIDKKDVPSLKGHIHFDNLYFRYTDKEPILNGLNLLIQPGETLALVGHTGAGKSS
                     IAKLIARFYEFQQGQLLIDGHDIRTFDLAQYRRQLGIVSQVPFLFSGTVADNIRYAVP
                     TATEEEILKMAREIGDGEWLETLPNGIYTEVGERGARLSMGQR"
     gene            complement(1544..3343)
                     /locus_tag="VFQ23_04690"
     CDS             complement(1544..3343)
                     /locus_tag="VFQ23_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9905908.1"
                     /translation="MTTISTPAEFSMQRTHLSDRTDPVRWVWSHVRRHWWIIIIMVIG
                     AIGNAALAAVVPVLTGDAFNAMLQPVPDTSVLIPLAIIIGTSQVIRGALQLGRNFGAE
                     ILAQRLERSVRDELYLSLLGKSMTFHNLQPVGDTMARSTNDVREVNYMFSPGINLVVG
                     SLIFLLMPIFVAGRYHPSLILTPVVFIVLYFVSLLRYLRTLGPITDQVRATFGKMNTH
                     LSESLDGVEVVKGASQENAEVERFVVNASRVRDTFVKQGDLEGRYVAMLLLGSAYAFG
                     LFHALLLFRNGTLNLGDVVAYFGLLRLLEFPTFVSTFAYSQISLGISGARRILELMNR
                     ETNLDQNAQGYADSMRGEVEFRNVSFNYNTHPESLPEQEKGEGEEVLQDISFKVKPGQ
                     TVAVVGQTGAGKTSLVKLINRTYDATQGQVIVDGVDVRDWNLAALRSQVSMIEQDIFL
                     FSRSVSDNIAFGKPGAEPDEVEMAARSAQADDFIQSFDKGYETVVGERGVTLSGGQRQ
                     RIALARAFLTDPHILILDDSTSAIDSATEDKIQRAIANAARGRTTFIITHRLSQIRWA
                     DLIIVLRKGKIAAIGTHDELMKTSDAYSRIFRE"
     gene            3704..3853
                     /locus_tag="VFQ23_04695"
     CDS             3704..3853
                     /locus_tag="VFQ23_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905909.1"
                     /translation="MNVSLPKTISGWCMWLFFLCVGLGMFVPVLATLAPWFALAYAVL
                     SLIGM"
     gene            complement(3903..>4354)
                     /locus_tag="VFQ23_04700"
     CDS             complement(3903..>4354)
                     /locus_tag="VFQ23_04700"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9905910.1"
                     /translation="KKLRALFNGGTGPLNEKLVDRVEQSTKDFFAENHKPLLMHGDFH
                     HFNILSSERGWLVIDPKGVVGPAGYEVGPFLINPWGEMSNEINQRQMTVRRIDILHER
                     LGFERERIREWGLAHAVLSAWWHIEDNTSGEAYAMNFAEMMASLPLS"
BASE COUNT         1109 a         1168 c         1065 g         1012 t
ORIGIN      
        1 tgtcgttgcc ccatcgataa ccgcgcgcct ctttctccga cctcagtgta aatgccgttt
       61 ggaagcgttt cgagccattc gccatcaccg atctcgcgcg ccattttcag gatttcctct
      121 tccgtggcag tcggcaccgc ataacgaatg ttatctgcta ccgttcctga aaaaaggaac
      181 gggacttgtg agacgatgcc caactgacga cgatactgcg ccaggtcgaa cgtgcggatg
      241 tcgtgtccgt caatcaataa ctgtccctgt tgaaattcat aaaagcgcgc gatcaatttc
      301 gcgatggacg acttccctgc accggtgtgt cccaccaggg caagagtctc tccgggttga
      361 ataagcaggt ttaacccatt cagaatcggt tctttatcgg tatagcggaa atagagatta
      421 tcaaagtgga tgtgaccttt taatgagggc acatctttct tatcgatctg aactacattc
      481 gggtcggcat cgatgagggc aaaggctcgt tccgccgcag aaagcccagc ctgaatctgt
      541 gcccagaatg cagagaggtt aagcaccgga aagaaaaatt gatcgagact catgataaac
      601 aaataccacg cgcccacggt aacaatccct tccgctgcgc tcaagcctcc cacataaatg
      661 aggatcgcag taaagattcc gcccaaggcg ttgagaaccg gaaagaccag cgagagcaca
      721 aagccgcgct gtacattcac ctgatacgac tgttggtttg attcatcgaa agatttgaaa
      781 atgctttctt cctgacggaa gttcttcgcg attgaaatgc cactgatcgt ctctttgatc
      841 gctgcattga catcagacat ggctttcatg ccacgcttgg tgacacggcg tgccagcacg
      901 cggaaaccac ctgccgccaa aaagataatt ggcagaaagg ccatcagcat caatgacagg
      961 cgcaggtcag tgcggaataa cacaacgccg agaataatcg cctggacgac ttgcgagcct
     1021 acatcggtca cgatgaccac cagttgcccg aagtcgttgg tatcggaggt aatgcgtgag
     1081 acgatccgcc cggacgagaa ttgatcatag aacgacaggt catgttccgc agccgctcga
     1141 aatgcgcgcg ttcgcaggtc gagaaccacg tcacctaccg cacgcacaac caggcttcgc
     1201 cgcgcccagt tcagtcccca taaaccaaac ccgatcatca aaacagccag accgataagt
     1261 gcgatctcct ggaacgacgg ctgctcgttc aatgaatcca ccatacgcgc aacgaccaca
     1321 ggcaaggccg cgccaatacc cgcaagaaca accaccagga cagtaacaaa aacaagccgc
     1381 ctggtctgtg atttgaaata ttcaaagatt cgacgcatca gttcacggtc agtgtactga
     1441 cgatcgtatt tttcgtcgtt taatcccgaa aagaagccca ttattatcct atgtaggggc
     1501 agcgtcttcc tgccccaggc gagacgacct cgcccctaca aaattactct ctaaatatcc
     1561 tcgaatacgc gtcagaggtc ttcatcaact catcatgggt tccaatcgca gcgatcttcc
     1621 ctttgcgaag cactatgata aggtctgccc atctgatttg cgacaggcga tgggtgatga
     1681 tgaaagtcgt ccgcccacgg gcggcgttgg cgatcgcacg ctggattttg tcttctgtag
     1741 ctgagtcaat cgccgaggtg gaatcatcca gaatcaaaat atgcggatcg gtcaagaagg
     1801 cgcgcgccag agcaatacgt tggcgctgtc ctcctgatag agtaacgcct cgttcgccga
     1861 cgactgtttc atatccctta tcgaaggatt gaatgaagtc atctgcctgt gcggacctcg
     1921 ccgccatctc cacttcgtct ggttccgctc ctggcttccc gaatgcgatg ttgtcgctga
     1981 ccgaacgtga gaagaggaag atatcctgct cgatcatcga cacctgcgaa cgaagcgcgg
     2041 ccagattcca atcgcgcaca tccacaccat cgacgatgac ctgtccctgc gttgcatcat
     2101 acgtacggtt aatgagcttc acaagtgacg tcttgcccgc acccgtttgc ccaacgacag
     2161 caaccgtttg cccgggttta actttaaagg agatatcttg caatacttct tctccttcac
     2221 ctttctcctg ctcagggaga gactcggggt gagtgttgta gttaaaactc acattccgaa
     2281 actctacctc gcctctcata gagtctgcgt agccctgggc attctggtcc aggttggttt
     2341 cgcggttcat caattccaag atgcgacggg cacctgagat cccaagtgag atttgagaat
     2401 atgcaaatgt ggatacgaac gtcggaaact ccagcaggcg cagcaaaccg aaatacgcaa
     2461 ccacatcacc gagattgagc gtaccattgc ggaagagcaa cagtgcatga aacagtccaa
     2521 aggcataggc tgaaccgagc aacagcatcg ccacatagcg tccttcgagg tcaccctgtt
     2581 ttacaaacgt gtcgcgcacg cgactggcgt taacgacgaa gcgctccact tctgcgtttt
     2641 cctgtgaggc accttttacc acttccacac catccagaga ctcagaaaga tgagtattca
     2701 tcttcccgaa cgtcgcacgg acttgatcgg tgatcggtcc aagggttctt agataacgca
     2761 ggagcgatac aaaataaaga acgatgaaga ccacgggcgt gagaatgagc gaggggtgat
     2821 atcgccctgc gacgaagatg ggcatcagca aaaagatgag cgaccctaca accaggttga
     2881 tccccgggct gaacatatag ttgacttcgc gcacgtcatt ggtggaacgc gccatcgtat
     2941 cacccacggg ctgcaaatta tgaaaggtca tgctcttgcc cagcaaagag agatacagct
     3001 catcacgcac agaacgttcc agtcgttgcg cgaggatttc tgcaccgaag ttgcgtccca
     3061 gttgcagtgc cccgcgtatc acctgcgagg taccaatgat tatggcaagc gggatcaaaa
     3121 cacttgtgtc tggaaccggc tgaagcatgg cattgaacgc gtcaccagtg agtaccggta
     3181 cgaccgctgc aagcgccgcg ttgccaattg caccgataac catgatgata atgatccacc
     3241 agtggcgtcg tacgtgcgac caaacccacc gcaccgggtc ggtacggtcg gagagatgcg
     3301 tgcgctgcat tgaaaattca gcaggagttg aaatggttgt catgaataat tgtgagaatc
     3361 aaaaatcgaa aggttttgcc gcagcatacg atcctgttat agagcgtttt actgctttga
     3421 agtttataca atgaagataa gcggcatctt tgggaagcaa aaggataact aaattctacc
     3481 atgtggcagg agccgtcttc gcgcttttcc agctatcacc tcgctgttga acctgtgaaa
     3541 gcatgaaaag cagatgtgac ttgaccatcg ccctcatctt cgaaacagaa ctggcattgt
     3601 cttcgagtgt gattgattcg tatctcttga aatctatcag ggttttaaat ggtagaactg
     3661 tccaacgtgg acggcccaga ctcactcaaa aggagagtgt aacatgaatg tttcgttgcc
     3721 caagacaatt tccggttggt gtatgtggtt gttctttcta tgcgtcggcc ttggaatgtt
     3781 cgttccagtc cttgctacgc tcgcgccctg gtttgccctg gcttatgcag ttctaagtct
     3841 catcggaatg taacggtctc aagttcttga agacagcctt ctgcccggga ggctgtttta
     3901 ttttaagaaa gcggcaggct agccatcatt tcagcaaaat tcattgcgta tgcttcgcca
     3961 ctggtattat cttcgatgtg ccaccaggca gaaaggacag cgtgtgccag tccccattca
     4021 cgaatgcgct cacgctcgaa ccccagtcgc tcatgcagga tgtcgatgcg cctcaccgtc
     4081 atttgtcgtt gattgatttc atttgacatc tctccccacg ggttgatgag aaacggaccc
     4141 acctcatagc ccgcaggtcc aacgactcct ttcgggtcga tcaccagcca gccgcgttca
     4201 gaagataaaa tgttgaagtg gtgaaagtcg ccgtgcatga gcaggggctt atgattctct
     4261 gcaaagaaat ctttcgtaga ttgctccact cgatctacaa gcttttcatt caaaggaccc
     4321 gttccgccgt tgaacagagc tcgtagtttt ttca
//
LOCUS       DASTJP010000154         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_159153, whole genome shotgun sequence.
ACCESSION   DASTJP010000154 DASTJP010000000
VERSION     DASTJP010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159153"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VFQ23_04705"
     CDS             complement(<1..948)
                     /locus_tag="VFQ23_04705"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905911.1"
                     /translation="MAELQLNSYREILKNKNFLAFWSGFTFSAVGDSLTRVALTWLVF
                     EETKSAQALGILTIAYTAPILLGGFFAGPLLDRFGARRVMIVDNLIRGIIFALIPIFD
                     VLGILEIWHIYVFAAIYGALMMISLAGGPTLIPSIVKQEQLETANAMETLSYTVSGVI
                     GPPLAGLLIVWIGTTNVVIFDAISYLVFAFTLWVMSVQEPIHQAVENEVVSYGIMDAV
                     RLMIRNKVLLSTTLMFMAANVGLGAMTVYLPLISDQIATGSSQVFGVLLGALALGEVL
                     SAWLAGSLILNMPLGKRIALAQILSGLSLALLLIGPSAFW"
     gene            complement(1096..2142)
                     /locus_tag="VFQ23_04710"
     CDS             complement(1096..2142)
                     /locus_tag="VFQ23_04710"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9905912.1"
                     /translation="MNTQLQSDVFYSALSRRDSAFEGIFVVGVKTTGVFCRPTCSARM
                     PKRQNVEFFRSAQEALAHGYRPCKICTPMEYQGAPPAWLKPLLHELAITPTLKLRDRD
                     LRERGLEPSRVRYWFKKQHGVTFQGYLRSIRISQAFGRIKQGGKVVDTAFESGYQSLS
                     GFADSFKKTTEFSPSESQNNQLIQTTRIATPLGPMLAAATEEGICLLEFTDRRMLEAE
                     IKRLSKLLNGRFVPGVSKHLDRLNRQLDEYFFGKRKEFDLPLVMPGTEFQKKVWMELQ
                     TIPYGITRSYKEQAEIIGAPRAVRAVAKANGNNSLAILVPCHRVVGANGELTGYGGGL
                     WRKEYLLNHESSFT"
BASE COUNT          615 a          626 c          562 g          530 t
ORIGIN      
        1 ccagaaagcc gaaggtccga ttaagagcaa tgcaagactc aaccccgaaa gaatctgtgc
       61 gagcgcgata cgtttgccaa gaggcatatt caaaattaga ctccccgcca gccacgcgct
      121 gaggacttct ccgagcgcaa gtgccccaag caaaacgccg aagacctgcg aactgccggt
      181 tgcaatttga tcagagatga gaggtagata gacagtcatc gcccctaatc caacgttcgc
      241 tgccatgaac atcaaggttg tggaaagaag cactttgtta cgaatcatca acctgacggc
      301 atccatgatg ccataggaaa ccacttcatt ttcaaccgct tggtgaatcg gctcctggac
      361 cgacatcacc cacaacgtga acgcaaaaac caaataagaa atcgcgtcaa agatgacgac
      421 attggtcgta ccgatccaaa cgataaggag acctgccagt gggggaccta tcacaccgct
      481 gactgtgtag ctcaaggtct ccatcgcatt ggcggtttcg agttgttcct gtttcacaat
      541 ggatggaatc aatgtgggtc cgccagcgag agaaatcatc atcaatgcgc catagatggc
      601 agcgaagacg taaatgtgcc agatttcaag gatgcccagc acatcaaata ttgggataag
      661 cgcaaatatt atcccgcgga ttaaattatc aacaatcatc acgcgccgcg caccaaaacg
      721 atccagcaga ggacccgcga agaatccacc caggagaatc ggcgcggtgt aggcgatggt
      781 taagattccc agtgcctgtg cagattttgt ttcctcaaat accagccagg tcagtgcgac
      841 gcgcgtcagc gagtcaccga cggctgagaa agtaaaccct gaccagaagg cgagaaagtt
      901 tttatttttc aggatctcac gataggaatt aagttgaagt tctgccattt aagattccct
      961 tactcgagat aataaacgtc atccgcttta aagtctcacc gcagagtcgc agagaccccg
     1021 gagaattttg aaagctttgc gaactctgca tctccgtgcg tagaaacatg cccctacgat
     1081 tcagagcaat gttggttaag taaagctaga ctcgtgattc aatagatatt cttttcgcca
     1141 taacccgcct ccgtagccgg tcagctcacc gttcgccccg acaactcgat gacatggcac
     1201 gagaatggcg aggctgttat tcccattggc tttcgctacg gcgcgaacag ctctgggagc
     1261 gccgatgatc tcagcctgtt ccttataaga acgagtgatt ccgtaaggaa tggtttgtaa
     1321 ttccatccat acttttttct gaaattccgt cccaggcatt accaggggaa ggtcaaattc
     1381 ttttcgcttt ccaaagaaat attcatccag ctgcctgttc agcctgtcaa ggtgtttgga
     1441 aacgccggga acaaaccgtc cattgaggag tttgctcaat cgtttgatct cggcctccag
     1501 catcctgcga tccgtgaact ccagcaaaca aataccttcc tccgttgccg cagccagcat
     1561 aggacccaag ggtgttgcaa ttcgcgtggt ttgaatcaat tgattgttct ggctttcgct
     1621 tggtgaaaac tcagtcgtct tcttaaacga gtctgcgaat ccgctcaacg attggtatcc
     1681 agactcaaaa gctgtgtcaa caactttgcc gccctgtttg attcggccaa acgcctgact
     1741 gatgcgaatc gaacgcagat atccttgaaa ggtgacacca tgctgcttct tgaaccagta
     1801 gcgaacgcgg ctgggttcca ggcctctttc gcgcaagtcc cgatcgcgca gcttgagcgt
     1861 tggcgtgatg gcaagttcgt gcaagagagg cttgagccag gctggcggcg cgccctggta
     1921 ttccatcggc gtgcagattt tgcagggacg gtatccgtgc gcaagtgctt cctgtgcaga
     1981 gcgaaagaac tccacgttct ggcgctttgg cattcgtgct gagcaggtgg gacgacagaa
     2041 cacgcccgtc gtctttacgc caacgacaaa gattccctca aaggcactgt cacgccgaga
     2101 cagcgcggaa taaaagacat cgctttgtaa ttgagtgttc atcgtgcctt atcctactca
     2161 tatcccgacg ctctgacaac cgaaaaatga acaagctcat tttttcctgg aaacgaatcc
     2221 gttctcaccg gactgtgagc cagactccta ccaaaacgac tgccagcccg atcaaacgcg
     2281 tcagttccat gggccggccc gccgcgccca acaagccata atgatcgaga atc
//
LOCUS       DASTJP010000155         7287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_3899,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000155 DASTJP010000000
VERSION     DASTJP010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3899"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..481
                     /locus_tag="VFQ23_04715"
     CDS             116..481
                     /locus_tag="VFQ23_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905913.1"
                     /translation="MNTDNPAIKLCIQGTQAEFQGLIDKARSLYQQAWDAAQDDYEAC
                     IAAHYMARHQENPKERLHWNQVALEKANAVADERVQAFYPSLYLNMGQSHELLGNKNE
                     AKRYYDLAAELGVIHQMET"
     gene            622..1254
                     /locus_tag="VFQ23_04720"
     CDS             622..1254
                     /locus_tag="VFQ23_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905914.1"
                     /translation="MQQSLQTEVSQLLEEVGRLAQLGKREKAYQASLRATSLAPDEPR
                     AWYLRSQNAPSTEEQLMCLSRTYSLDPNFPNTQGELRAAMQTLLTQEPFLAYVHETES
                     LYQVRSGRDLLINVPKNRAFETPYLKKAPGLANPAFRWLSFALLGLFLGGVGAVVFAP
                     IAAFQALRLQVSAPSHGERNRLLIVFILAVIIWLAAIPISWLLLIRFYSS"
     gene            1260..3572
                     /locus_tag="VFQ23_04725"
     CDS             1260..3572
                     /locus_tag="VFQ23_04725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9905915.1"
                     /translation="MLVRLLYLSALWFAISLFATLILGSRLRKRDSILSLLWLTGVIL
                     TGNLIAIFTFGRLLEIFGFSILAFGFGLLWIIQLQDWNAAGQITWTMTLITTALFMVY
                     TFLLTAFSPLNPLSFVFALLFFFLEAVTLLMALAHMHESLDVVCRVQWHRLIEKLEPI
                     PGYEPMVSLQVPAYNEPVEVIHKTLISLAGLNYSNFEVLVVDNNTPKQETWRPLEEIC
                     RQLGPNFHCLHLDQWPGYKSGALNFAATQTDPRAEIIAIIDADYEVDPNFLRELVPAF
                     ADPKVAFIQTPQDYNDSTGDTFSESMYHGYKYFFVVSMPSRNERNAIIFAGTMGLIRK
                     SVLEEIGGWDEWCITEDAEASLRILKKGYTSLYMNRSFGSGMMPINFEGLKKQRFRWC
                     FGGIQILKKHWEALMPWAHLVDPQNKLTLAQRYYYLVGGLGWFSDVFNLLFALFLVMG
                     AIFNLFSSPVKVRPLTDTVLIIPAVFLLLHILRFIWVLRNRLRVSVDMAVATMYNFFS
                     LGWSVTLACIQGLVQREGVFLRTPKSTSASKAWRAIQVTQWETTIGLVCVIVGLLAFG
                     FERNWRTFSLCILLIWQGSLYLAAPLYSLLSMKREAALGAPVRVADQGKPLMEQLAAR
                     WVLACSMVLISAFSLIRFLPSPTNLPDYARFLPIDLSAEEIVAGPSEPEVAGTEIPET
                     LVTPTLPATVSGIATVKVESANCRAKPRGGAGRVTYLYNGQQVEILGRNEDLVNPWWY
                     IKIPDSGGNCWLWGMTTTFTGNIEDIPIIR"
     gene            3892..4278
                     /locus_tag="VFQ23_04730"
     CDS             3892..4278
                     /locus_tag="VFQ23_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9905916.1"
                     /translation="MIRVAISEDHPQVRVALRLLLSQSNGIELISETENGQEAVDCVK
                     VLQPDVLVMAIHLPVLDGFEATKQITRLQVSTRVILISVDIGSFIAMQAADVGARGFI
                     PKDNLAQQLLPAIEAVQQGETFFVES"
     gene            4322..5551
                     /locus_tag="VFQ23_04735"
     CDS             4322..5551
                     /locus_tag="VFQ23_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905917.1"
                     /translation="MNIGIHFLTTLSHIAARSPLFFRLPKVRSNLYWVLIIGVALTSC
                     TIDVSQSSEVGPVSGTATPFLVAPSPTLPPGVTPVETPPAAISTSIPVTWSALNLTGS
                     LIYISPPVAGKAAFFNSIRRLDLLTGEIRTLFTTAGNDWIYYVSVSPDAEQLIMSYLP
                     IPESGTLPNRALYQIPLDGTTPPQLLFSPPTSDDHYIHVEWSPDGKFIYYSHLNYNVR
                     LPNGVDPVYDIFRMSYPEGQTEKIADYAFWPRLSSDSTRLVYVAINPDTAKNELYVAN
                     ADGSNPQQIVLSGATPPDIIDAPIFSPDGSSVIFSAPPPVQAYQPNWFDKLMGIRIVK
                     AHEIPSDWWSVPVTGGTPTQLTQLQTIRLFASLSPDKRHVASLGGEGIFVMDETGSNL
                     TQLLLEPGVSGTVSWIP"
     gene            5862..6437
                     /locus_tag="VFQ23_04740"
     CDS             5862..6437
                     /locus_tag="VFQ23_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905918.1"
                     /translation="MKKSSITIAFTIALITLLFNVRLALAHEGVTVGDYEIVVGWVNE
                     PPVAGQMNGIELHVSNNSSGDEQPVEDITSLTVTVSYGGQSKELTFEPAGPDAPGEFH
                     AALLPTIPGQYTVSFGGQLGDTPVDAHTEPEEVAQAETIQFPVSYPNPQSEASARTES
                     AARGTSEWLVWLSLLIGLVGVGLGVTALRKK"
     gene            6434..>7287
                     /locus_tag="VFQ23_04745"
     CDS             6434..>7287
                     /locus_tag="VFQ23_04745"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HET9905919.1"
                     /translation="MKLKKKSWFLIVVLLYCAVTVRSVSAHALLVSSNPEANAVLQQA
                     PVQVEMIFSETLEPNLSSVTVFNSSNQTVDAGDVRVDPSDPRRMTVTLHSLPDGVYTV
                     SWKALSTVDGHQTVGTFPFAVGDANAESMQSMQQSTTARLPFSALLSKFILLVSLTVL
                     IGQRLFIWLIWNPALRSAQNVSPEISQPEIWHTLYRIGLIGVLLGIGIGLLAQAGQTT
                     GRELSFPWHPEVGRILAETRLGVIWLTRLALAVFAVWLAGAKESLLRNWLVLVVHLAL
                     LFTVTMTS"
BASE COUNT         1697 a         1810 c         1863 g         1917 t
ORIGIN      
        1 ttgctggcaa ttggcaacac cacaagtcat attttattcg ggcttggtgc agtctggctg
       61 ggtcgctcag ttttagatct aatttggcga taaccccgtc gcatctgggt gaactatgaa
      121 taccgataac cccgccataa aactttgtat ccaaggtacg caggctgaat ttcagggatt
      181 gattgataag gctcgttcct tatatcaaca agcatgggac gccgcgcagg atgattatga
      241 ggcttgcatt gccgctcact atatggcgcg tcatcaggag aatcccaaag aaagacttca
      301 ctggaatcag gtcgcgctcg aaaaggcaaa tgccgttgcc gatgagcgcg ttcaggcctt
      361 ttacccatct ctttacttaa acatgggtca gtctcatgaa ctactcggca acaaaaacga
      421 agcaaaacga tattatgacc tggcagcgga attgggggtg attcaccaga tggaaacgtg
      481 aacagaatga cgatcaacct gaaaagcgtt agttaccgaa ttgatatttg ttatggttgc
      541 taattctgtt atcatcacag agtaaattcg tctttcgtta agtcatagta ggcaaatcag
      601 atattttcag gaacctttat tatgcagcag agtctccaga ccgaggtctc tcagttgctg
      661 gaggaagtgg ggagacttgc ccagctaggg aaacgtgaga aggcttatca agccagctta
      721 agagctacca gtcttgcacc agacgaacca cgcgcctggt atttgcgctc gcaaaatgca
      781 ccctccacag aagaacaatt gatgtgcctg agccgcacgt attccctgga tccgaatttt
      841 ccgaataccc agggagaact gcgcgccgcg atgcaaacgc tgctcaccca ggaaccgttc
      901 ctggcgtacg tccatgagac cgagtccttg tatcaggtga ggagtgggcg cgacctgctc
      961 atcaacgtcc ccaagaacag agcgtttgaa acgccctatc tgaaaaaagc gccagggctt
     1021 gccaaccctg cttttcggtg gctgtctttt gcgttgctcg gcttgttcct gggcggcgtg
     1081 ggagccgtcg tattcgctcc gattgcggcc tttcaggcgc tgcgtcttca ggtatccgct
     1141 ccgtctcacg gtgagcgcaa tcgattgttg atcgttttta ttctggcggt gatcatctgg
     1201 ctggcggcga tcccgatcag ctggctgctg ttgatccgct tttattcttc gtgacgtaaa
     1261 tgcttgtacg actgctgtat ctctccgccc tttggtttgc catcagctta tttgcgacgc
     1321 tgatcctggg atcgcgtttg cggaagcgcg actcgatcct ctccctgctc tggctgaccg
     1381 gtgtcattct gacggggaat ttaattgcta tcttcacctt cggcaggctc ctcgaaattt
     1441 ttggtttttc catcttagcc ttcggcttcg gacttctctg gatcattcaa ctgcaggact
     1501 ggaacgcggc ggggcagatc acctggacca tgacgttgat cactactgca ctgtttatgg
     1561 tctatacgtt cttattgact gccttttcgc cgctgaaccc tttatcgttt gtctttgcat
     1621 tgcttttctt tttccttgaa gcggtcacgt tgctgatggc tctggcacat atgcacgaaa
     1681 gcctggacgt cgtctgccgc gtccaatggc atcgcctgat cgaaaagctt gaaccgatcc
     1741 ctggctatga gccgatggtg tcgctacagg tcccagccta taacgaacca gtcgaggtaa
     1801 ttcataagac gttaatttcc ctggccgggc tgaattattc caacttcgaa gtgctggtgg
     1861 tggataacaa cacgcccaag caggaaacct ggcggccttt ggaagagatc tgccggcagc
     1921 tcggccctaa ctttcactgc cttcatttgg atcaatggcc ggggtacaaa tcgggcgcgc
     1981 tgaactttgc cgccacgcag acagacccgc gtgctgagat cattgcgatc atcgacgcag
     2041 attatgaagt ggatccgaat tttttacggg aactcgtccc agcctttgcg gacccgaagg
     2101 ttgcatttat ccaaacaccg caggactata acgattccac gggagataca ttctcggaat
     2161 ccatgtacca cggttataaa tatttcttcg tagtttccat gccctcgcgc aacgaacgca
     2221 atgcgatcat ttttgccggc accatggggc tgatccgcaa atcggtgttg gaagagatcg
     2281 gcggctggga tgaatggtgc atcaccgaag atgccgaagc cagcctgcgc atcctcaaaa
     2341 agggatacac gtccttatat atgaatcggt ccttcggcag tgggatgatg ccgattaact
     2401 tcgaaggtct gaagaaacag cgtttccgct ggtgctttgg cggtattcaa atcctcaaga
     2461 aacattggga agccctgatg ccctgggcgc atctggtgga ccctcagaat aaattgaccc
     2521 ttgcccaacg ctattattat ctggtgggtg ggctgggttg gttctctgat gtattcaact
     2581 tattatttgc tttattcctt gtgatggggg cgatcttcaa tttattttcg agcccggtaa
     2641 aagttcgtcc gctgaccgat acggtgctga ttatccctgc tgtttttttg ctactgcata
     2701 tcttgcgatt tatctgggta cttcgcaacc gattacgcgt ttccgtagat atggctgtgg
     2761 cgaccatgta caactttttc agcctgggct ggtctgtaac gctggcttgt atccagggcc
     2821 tcgtacaaag agaaggcgtc tttctgcgga cgcccaaatc cactagtgct tccaaagcct
     2881 ggcgcgcgat tcaggtaacg caatgggaga cgacaattgg gttggtttgc gtcattgtag
     2941 gtttactggc atttggcttt gaacggaatt ggcgaacgtt tagtttgtgc attttgttga
     3001 tctggcaggg gagtttgtat cttgccgcgc cgctttacag cctgttgagt atgaaaaggg
     3061 aagccgcgct gggcgcaccg gtccgcgttg cagatcaggg caagcccctc atggaacagc
     3121 tcgccgcgcg ctgggtcttg gcctgttcca tggtgttgat cagcgccttc agcctgatcc
     3181 gatttctccc ttcgcccacc aacctgcctg attacgcgcg attccttccg atagatcttt
     3241 cggcggaaga aatcgttgcc ggtccgtccg aacctgaagt cgcgggaaca gaaattccag
     3301 aaacattagt cacgcccaca ttgcctgcta ccgtgagtgg tatcgccacg gtcaaagtgg
     3361 agagcgcgaa ctgccgcgcg aagccaagag ggggtgccgg gagagtcact tacctctata
     3421 acggccagca ggttgagatc ctggggagaa acgaagatct tgtgaatcca tggtggtata
     3481 taaaaatacc ggactcgggc ggtaactgtt ggctatgggg tatgaccacg acatttaccg
     3541 ggaatatcga agatattccc attatccgat aatgataatg cccgtaaagt aattgaatac
     3601 gactcaaata ctcacctcaa aagtgagcat ttgagtttgt gaaaagggtt tggaaaacta
     3661 gaatattgca gcctgatttg cagcctacct ggtactaaaa ttacccttca tcaattgggg
     3721 aatataccta tcacgacggg gaatactcct gatagacatg gtcctccact ttaattagaa
     3781 ttggtacgca aatacttgtg tcgactcttg tgcggggact ataagatggt gcaccaccat
     3841 attgattggt tgtggcatca agatacgtta tgcgtataaa agagtaacat catgatccgt
     3901 gttgcgattt cggaagatca tccgcaggta cgagtagctc ttcgattgtt gttgagccaa
     3961 tcgaacggca tagagcttat tagcgaaaca gagaatggtc aggaagcagt ggattgtgtt
     4021 aaagtcctcc agccagacgt tctcgtgatg gcaattcatc tgcctgtctt ggatgggttt
     4081 gaagctacaa aacaaatcac cagattacaa gtttcaacgc gtgtgattct gatctcggtg
     4141 gatattggaa gttttatagc catgcaggcc gctgatgtcg gcgcgagggg ctttataccg
     4201 aaagacaatt tggcacagca gttattgcca gcgattgaag cagtacagca aggagaaact
     4261 ttctttgtgg aaagttaaat aatcagcatt cccaactcca atccattaga ataaagccaa
     4321 tatgaatatt ggcattcact tcctcacaac cttatctcac atcgcagccc ggagtccgct
     4381 attttttcgg ctgcctaaag tccggtcaaa cctctactgg gtgttgatta tcggcgttgc
     4441 tcttacttct tgcacaattg atgtaagtca gtcttcagag gtaggtcctg tttccggaac
     4501 agctacgcca ttcttggtag cgccgtcacc taccttgccg ccgggcgtga ctccggtgga
     4561 aacacctcct gctgcaattt ctacgtctat tcccgtcacc tggtctgcct tgaacctgac
     4621 ggggagtctg atctacatca gcccaccggt ggcgggcaaa gccgccttct tcaacagtat
     4681 tcgaaggctg gacctcctca caggtgagat ccgcacattg tttacaaccg ccggcaatga
     4741 ttggatttac tatgttagtg tttcgccaga cgcggaacaa ctgatcatgt cttatttgcc
     4801 aatcccagag agcggtactc tgccaaatcg cgcgttatac cagatacccc ttgatggaac
     4861 aactccaccg caactactgt tttcaccgcc gacctctgat gatcattata ttcatgttga
     4921 atggtcgcca gatgggaaat ttatttatta ttcccacctc aattacaatg ttcgtcttcc
     4981 caatggggtc gaccccgttt acgatatttt tcgaatgagt tatccggaag gtcaaacgga
     5041 aaagattgcg gattatgcat tctggccgag actttcctct gattcgactc gtcttgtgta
     5101 tgtcgcgatc aatcctgaca ccgcaaagaa tgaactgtat gtggcgaatg cagatggaag
     5161 taatcctcag caaattgtgt tatccggtgc gactccgcca gatatcatcg atgctccgat
     5221 tttttcaccg gacggaagct ctgttatttt tagcgcgccg cctcctgtgc aggcctatca
     5281 accaaactgg tttgataagt taatgggaat tcgcattgtt aaagcacacg aaatcccctc
     5341 cgattggtgg tcagtgcctg tgacgggggg aacgcctaca caattgacac aactgcaaac
     5401 cattcggctt tttgccagcc tctcacccga taaaaggcat gttgccagtt tgggcggcga
     5461 agggatcttt gtgatggatg agacaggttc gaatctaacc cagttgcttc tggaaccagg
     5521 ggtctccggc accgtcagtt ggattccctg atccggtcat cccgtgttcc tgtttcaaca
     5581 ttatctttga cgactctgag atccatcgtt ggctgataag gttatcgctc cacgaaaagc
     5641 ttggaattaa acatatcgta ggggaaatat tagctcaaga tgaaagcaac tgaacgtaag
     5701 atgattcgct gattatcata attgcaaagg cgacccagcc gtcaccgctg tgcagaaaga
     5761 gtcgcggttg aatacaatag tcagcgtttc atcatgaata acacactttt ccagatgcaa
     5821 aagtgctgta gaattcccca atctcgaaac gaggagcctt catgaaaaaa tcgtctatta
     5881 cgatcgcgtt cactatcgct cttatcactc tgctctttaa cgttcgactc gcactggcac
     5941 acgagggagt cacagtcggt gattatgaga tcgttgtggg ctgggtcaat gagccgccgg
     6001 tggcgggcca aatgaacggg atcgaactcc atgtatcgaa caacagcagc ggcgatgaac
     6061 agcctgtaga agatatcact tcgctaaccg tcacggtttc ctatggtggt cagagtaagg
     6121 agcttacctt cgaaccagct ggcccggatg cacctggcga atttcacgct gcgcttcttc
     6181 ctacgatccc gggccaatat actgtaagct tcggtggtca actcggagat actcccgtgg
     6241 atgctcatac ggaaccggag gaagtagcac aggccgaaac gattcaattc ccggtgtcgt
     6301 atccaaatcc acaaagcgaa gcatcggcaa ggaccgagag tgcagcacgc ggaacctcag
     6361 agtggttggt ttggctcagt ctgttgatcg gtttggtcgg agtcggattg ggtgtaacag
     6421 ctctccggaa aaagtgaaac tcaagaaaaa gtcctggttc ctgatcgttg ttctgctgta
     6481 ctgcgcagtc acagttcgat ctgtttctgc gcacgcgctg ctggttagtt caaacccgga
     6541 ggcaaacgct gttttgcagc aggccccggt tcaggtggaa atgatcttca gcgagacatt
     6601 ggaaccgaac ctcagttcag ttacagtctt taattccagc aaccagaccg tggacgcggg
     6661 cgatgtgcgc gtcgatccct ccgacccgcg gcggatgacc gttacgctgc attccctgcc
     6721 ggatggagtc tatactgtca gttggaaggc cctttccacg gtcgatgggc atcagactgt
     6781 gggcacgttt cccttcgcgg taggggatgc aaatgcagag tcaatgcaat ccatgcagca
     6841 gagcacgaca gctcgcctgc cgtttagcgc gctgctttcc aagttcatct tactggtctc
     6901 actgacggtt ctgatcggtc aacgactttt tatctggttg atttggaatc ccgcgttgag
     6961 atcagcgcag aacgtttcac cagagatcag ccaacctgaa atttggcaca ccttatatcg
     7021 aattggtttg atcggagtct tgcttggaat aggaatcggg ctgctagcgc aggcgggtca
     7081 aacgaccggg cgtgaactgt cgtttccctg gcatcccgag gtggggcgaa tcctggctga
     7141 aacgcggctg ggagtcatct ggttgaccag gttggccctt gccgtgtttg ccgtttggct
     7201 ggcaggggcg aaggagtcac tgctccggaa ttggctcgtg ctggtcgtcc atctcgcact
     7261 gttgtttaca gtgacgatga ccagtca
//
LOCUS       DASTJP010000156         4696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1388110, whole genome shotgun sequence.
ACCESSION   DASTJP010000156 DASTJP010000000
VERSION     DASTJP010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388110"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFQ23_04750"
     CDS             complement(<1..1015)
                     /locus_tag="VFQ23_04750"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9905920.1"
                     /translation="MSHSVFISYSRRESPFVDILLDELEDEGVNVWVDYHCLIPAKPW
                     PDQILEGIRQANVFLLVVSKASMASENVEVEYKHALEGKKRMILLIFEAVDLPPALQT
                     CEWIDFRSSFGRKKRELLVRLDGPIEQPAPPQGGFRTSLTVWLTFFLSVFTLLLSIPA
                     WWTFYIPALLIPLPSRILRRDFHFYRVRFAVLTLPIVLLLSWVFFSTYSYLSNLFIAP
                     AAISFFTSPVLLLLLSSKGMRLWGKPIASAPRFANPYQPEVNTPQPVPFFIEHAPQDK
                     KYADAIRTELANYGHPLVTVPVEAKANFVLLSRYKDSTALDPETQVVYPILIQDTPIE
                     DKKL"
     gene            complement(1122..1748)
                     /locus_tag="VFQ23_04755"
     CDS             complement(1122..1748)
                     /locus_tag="VFQ23_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905921.1"
                     /translation="MNDFDSIFDESWSQIESGESTVDEILARHPEHRAQLGPLLQVAS
                     KLAGTKDVAAPSPSFRARNRAQLNRYMYENPQMKRVSPLFWRMTITLVSLLLAFIVSG
                     TVFAQSALPGDAFYYWKRSSESAWRIVSPDRLGTELNLSNRRVHELVALSGNEIGRER
                     VLDDYLRLLVNFEDEKDQNRILIALQAQKEFLENAGVSIPELNKYLMP"
     gene            complement(1745..2326)
                     /locus_tag="VFQ23_04760"
     CDS             complement(1745..2326)
                     /locus_tag="VFQ23_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9905922.1"
                     /translation="MNSESVKSSRLPLENEQQLVLRAQDGDAEAFGQLYDAYMERIYR
                     FIYFRVDDQQTAEDITSQVFLKAWSNLHRFRFSRTPYLAWLYTIAHNTVIDHYRTRKP
                     ATALEDVQLSQPDYSEAVENEIDLAVEMKSIKAAMRNLTSDQQQVLTLRFIDGMSNTE
                     IAQHLGKREGAIRALQMRGLQALAKQLAEKMVT"
     gene            complement(2557..2826)
                     /locus_tag="VFQ23_04765"
     CDS             complement(2557..2826)
                     /locus_tag="VFQ23_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905923.1"
                     /translation="MSKSFAKVAFALLLALILIIAIYTSVQGAWLNAGTRIGQSHVDA
                     GLKADFSRSRSAGVSELSSFEAQPDTFKQDGGHGCESENSNPSDF"
     gene            complement(3072..4037)
                     /locus_tag="VFQ23_04770"
     CDS             complement(3072..4037)
                     /locus_tag="VFQ23_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905924.1"
                     /translation="MMLLRKSILLLSLFALTACLQKPVFNIEVVQPASTAEFIASATP
                     FTAWTEMAMPSPTPVIDEALPIFSPTPVVWSGEFSPVLYGGNRYGMSVFLLLGGVATG
                     EWITPETSALRFGGEATYSLHTLTQISRYFIWGQVPQFSYACGNYFVHTDPGLDEPGF
                     VGVLDGWNVTKREQIELSANDLLYQQTVIDFLTAMGVAAFPPSTLQAFRVDIEGDGVD
                     EVFISATHLDDPKQTPKGGDYSIVLMRKVVGNDAVTKLVVGDHYNAPRPEISHPRTYT
                     LANFIDLNQDGVLEVVVDVKKWEGFGGIVLEVNGQDVTARLSAEC"
     gene            complement(4185..>4696)
                     /locus_tag="VFQ23_04775"
     CDS             complement(4185..>4696)
                     /locus_tag="VFQ23_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905925.1"
                     /translation="GVKPGQSVIFRPVQTMAQDSGPYEQYLQEWLLLQGIQSPELVIK
                     NILRVDLEGDAVDEVVLVATNIENSMGGLHMAGAGKYSVVLMRKVIGAQVHTVPLVAD
                     IYYNTSPSMNYPIIYDVDHIYDLNGDGTLEIIISGQWWEGGGLFVHEVHGLNAPRVLD
                     LHCGIFVRR"
BASE COUNT         1213 a         1208 c         1135 g         1140 t
ORIGIN      
        1 gaagcttctt atcttctatg ggggtatcct gaatcagtat cggatagacg acctgtgttt
       61 ctgggtcgag agctgttgaa tccttgtaac gcgagagcag gacgaaattt gcctttgcct
      121 ccaccggaac ggtaaccagt gggtgcccat aatttgccaa ttctgtacgg atggcgtcgg
      181 cgtatttttt atcctgaggg gcgtgctcaa taaaaaaggg aacaggctgt ggagtattca
      241 cttcaggctg atacgggttg gcgaacctcg gagcagaagc aattggcttg ccccataacc
      301 gcatcccttt agaggagagc agtaagagca acaccggtga agtaaaaaag ctgatggccg
      361 ccggagcgat aaataggtta cttaggtaag aatacgtgga gaagaatacc cagctcaaca
      421 gcaggacgat gggtaaagtc aacaccgcaa aacggacgcg gtaaaagtga aaatcacggc
      481 gcaaaatccg actcggtaag gggattaata gagccgggat gtagaacgtc caccaggcag
      541 ggatggacag gagaagtgta aacacgctca ggaaaaaggt tagccaaacg gtaagggatg
      601 ttctgaatcc tccttgtggg ggagcaggtt gttcaatcgg tccatccaga cgtaccagta
      661 actctctttt ctttctccca aacgaactgc ggaagtcaat ccactcgcag gtctgaagag
      721 caggaggcag gtccaccgct tcgaaaataa gcaggatcat gcgcttcttg ccttcgagtg
      781 catgtttgta ttcaacttca acattttccg atgccatcga agctttcgaa acgaccagca
      841 aaaagacatt cgcctgacga atgccttcta ggatttgatc gggccagggt ttggcaggta
      901 tcaggcagtg ataatccacc caaacgttta ccccctcatc ctccagttca tccaacagga
      961 tatcgacaaa cggggattcg cggcgagagt aactgataaa aacagagtgt gacatgctat
     1021 gaatttctgt gatttcattg tatacccaaa aaataaggac acgaagctcg tgtccttaca
     1081 gactcattac tcttgaagtc actctctgcc gagaggttgt tttaaggcat caaatattta
     1141 ttcagctcgg ggattgaaac cccggcattc tctaaaaatt ccttttgtgc ttgcagtgcg
     1201 atcaaaatcc tgttctggtc tttctcgtcc tcgaaattga ccagaagccg cagatagtca
     1261 tccagcacgc gctcacggcc gatttcattg cctgaaagcg cgaccagctc atggacgcgt
     1321 cgattcgaca ggttgagctc ggttccgagt cggtcagggg atacgatccg ccaggcactc
     1381 tcactgcttc gtttccaata gtaaaacgca tcgccgggca gggcgctttg ggcaaagacc
     1441 gtgccgctca caatgaacgc cagaagcaga gaaacgagcg tgatggtcat tctccaaaat
     1501 agaggagaga cccgcttcat ctgcgggttc tcatacatgt atcggtttag ctgcgcacgg
     1561 ttgcgggcac gaaagctggg agatggagca gccacatcct tcgtgcctgc cagtttcgag
     1621 gcgacttgaa ggagcggccc aagctgggct cgatgctcgg ggtgtcgcgc caggatctcg
     1681 tccacggttg attcgccgct ctcgatctgg ctccaacttt catcgaaaat tgaatcaaag
     1741 tcgttcatgt gaccatcttt tctgccaatt gcttggcgag cgcctgcaag cccctcatct
     1801 gaagcgcgcg aatcgctccc tcgcgcttgc cgagatgctg tgcaatttca gtatttgaca
     1861 ttccatcaat aaacctcaag gtcagcacct gctgttgatc gctcgtcagg tttcgcatgg
     1921 ctgccttgat cgatttcatt tcgactgcca ggtctatttc attctccaca gcttcggaat
     1981 agtcaggttg tgacaattgt acgtcctcaa gggctgtagc cggttttcgc gttcggtaat
     2041 gatcaatgac ggtattgtgg gcgatggtgt agagccacgc aagataaggg gtccggctga
     2101 agcgaaagcg gtgcaggttg ctccaggctt tgagaaaaac ctgagaggtg atgtcttcag
     2161 ccgtctgctg gtcgtccaca cgaaaataaa taaaacgata aatgcgttcc atgtaggcgt
     2221 cgtagagctg tccaaacgcc tcggcatcgc catcctgcgc ccgaaggaca agctgctgct
     2281 cgttttccag gggcagtctg gaacttttga cactttctga attcactagg ctttgcctca
     2341 tataataacg atatgccctt aggactgtta caaaaaacac cctctcaggg ttagagagag
     2401 tgttgtgaaa ttcagcctgg atcccttcca attttgatag gtctgcagcc gcatcaagat
     2461 gcctaaccga ttaggtttcg ctattatatc atttgtatgc ctattaaaac aaaaaggggc
     2521 aatccgtgga ttgcccctgg ttttcttcgt cgaattttag aaatcgcttg gattgctgtt
     2581 ttcggactcg cagccgtggc ccccgtcctg cttgaaggtg tcaggctggg cttcgaagct
     2641 gcttaattca gacacgccgg cactccgact gcggctgaaa tccgccttca agccagcgtc
     2701 cacgtgggat tgcccgatcc ttgtgccagc attgagccag gcgccctgca cactggtgta
     2761 tatcgctata atcaggatta aggctaataa tagagcaaat gcgactttgg cgaatgactt
     2821 agacatggtc atggtagcta tctccttctg tttgctgaaa gaagcaggtt ttcttattta
     2881 gaaaaccctg agcagtgcga acgtattggc caccttaaca ccccgcttga aaagacttta
     2941 aagattatac acaattgacc atgggttggc aacaatccta aagagactct aatctacgaa
     3001 cgtacgttaa tagtgaattt gtcggtaaac gaacgaggag attcgccttc cgatccgaaa
     3061 ctcctccagg gctaacactc agcgctcagc ctggcagtca catcctgccc attgacctcc
     3121 agcacaatcc caccaaaccc ttcccatttt ttgacatcca ctactacttc cagcacccca
     3181 tcctgattca gatcgatgaa attcgccagc gtataggtcc gcgggtgaga aatctcgggc
     3241 cgtggtgcat tatagtgatc gccaaccacc aattttgtga cagcgtcgtt cccaaccacc
     3301 ttgcgcatca aaacgatgga ataatcacct ccctttggcg tttgcttagg gtcatccagg
     3361 tgcgttgcac tgataaagac ctcatctact ccgtcgcctt cgatatccac gcgaaatgcc
     3421 tgcagcgtac ttggcgggaa cgcagccaca cccatggctg tcaggaagtc gatcacggtc
     3481 tgttgataca agagatcatt tgcagaaagc tcgatctgct cacgctttgt gacattccaa
     3541 ccatccagga cgcctacaaa tcctggttcg tcgagcccgg gatccgtatg cacgaaataa
     3601 tttccacatg catacgaaaa ttgtggcacc tggccccaga taaaatacct ggagatctga
     3661 gtcaacgtat gcaggctata cgtggcctcc ccgccaaagc gaagcgcact tgtctcaggt
     3721 gtaatccact cacctgtggc cacaccgccc agcaaaagga agactgacat accatagcga
     3781 tttccaccgt agagaaccgg tgaaaattca ccagaccaca ccacgggggt tggtgaaaag
     3841 ataggtaggg cttcgtcgat caccggagta gggctgggca tggccatctc cgtccaggcg
     3901 gtgaacggtg tagcagacgc aataaactcc gctgtggaag ccggttgaac cacttcaata
     3961 ttgaagaccg gtttttgcag acaggcggta agagcgaata aagacaaaag cagaatcgat
     4021 tttcgcagaa gcatcataga atttttcctc tagtagtgat gacaactaca acggtatttt
     4081 tctttgcatt gttccaattc gtagaattac ttcgtgatta agaaccttcc ctgagcatga
     4141 atgactaaat aagaaaagac ctcaaacccg agttgggttc acatctatcg tcgaacgaaa
     4201 atgccgcaat gaagatccag gactctcggc gcgttcaaac cgtgcacttc atgaacgaac
     4261 aagccaccac cctcccacca ttgcccggaa atgataatct ccaaagtccc atccccgttg
     4321 aggtcataga tgtggtcaac atcatagatg atcgggtaat tcatcgaagg ggatgtattg
     4381 taataaatat ctgcaaccaa aggaactgta tgtacctgtg ctccgatcac ctttcgcatg
     4441 agcaccactg aatatttccc tgctcccgcc atatgcagcc ctcccattga gttttctata
     4501 ttggttgcca cgaggaccac ctcatccacg gcgtcccctt ccagatctac ccgcaagatg
     4561 ttcttgataa ccaactcggg ggattgaatg ccttgcagta aaagccactc ctgtaagtat
     4621 tgctcgtacg gtccgctatc ctgcgccata gtttggaccg gtcgaaatat cacagattgc
     4681 ccgggcttta ccccca
//
LOCUS       DASTJP010000157         8128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_658102, whole genome shotgun sequence.
ACCESSION   DASTJP010000157 DASTJP010000000
VERSION     DASTJP010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658102"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VFQ23_04780"
     CDS             <1..839
                     /locus_tag="VFQ23_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905926.1"
                     /translation="LYMPHPRVSKKPVGRLTRGKTASNRLRRVDNFILLYEPSLLPRT
                     EGLFADSLFVDLGYGFDARTTLESALRFRRVNPGLRVLGVEIDKDRVEAALPYADKKT
                     FFRLGGFNLPLSDGEHVRLIRAFNVLRQYEEKDFAPAYDRLAAYVLPHGLMIEGTSNP
                     FGSIWVANLVRKEESGHWRFEALVFSTNFRTGFEVEEFQTILPKNLIHHVAPGEEIYD
                     FFEAWKQAAKGTASMKNYGTRQWFIAAAEHLAGQGYDIESQRKWLSKGYLIWKQNERN
                     KS"
     gene            913..1794
                     /locus_tag="VFQ23_04785"
     CDS             913..1794
                     /locus_tag="VFQ23_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9905927.1"
                     /translation="MKKSNPFLFLAAVLLIVSLACVLPSTVTPDPNFISTAIAQTIVA
                     GVSQTGAVPSDVPIILESPTSTQTFTPELPTLSPTVTLTPSPVFTATPLVPLISVSVA
                     TNCRIGPGRQYDRVGALLVGEVAEVYGRDPTGAYWYIRNPDPGPEFCWLWGEYATLVG
                     NTGALPIYTPPPTPTPAPNFDPVYLGRDTCTGGWWLEFELKNTGGIQFKSFSITVRDT
                     DTDIVLSLYADGFTDRDGCSTSVTRDNLNPGDSRIVSSPSFGYDPIGHDIRVTVTACS
                     GLGQTGICVSKVINFDA"
     gene            complement(1858..3399)
                     /locus_tag="VFQ23_04790"
     CDS             complement(1858..3399)
                     /locus_tag="VFQ23_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905928.1"
                     /translation="MKFNARQLLNISLLLLFGFVLLRTAWVGDDAYITMRTVDNLLNG
                     YGPTWNVGERLQTFTHPLWMFILTIVYAITRDAYISLLGLSLFVSLVVIWIFLKHTSE
                     DTYSVFIGWVILLLSNASLDYSTSGLENPASHLLVLLFTLAYLKWNDQRSQNRILILA
                     LLAGLLTLNRMDLLLLCLPALLDLVVPVETGRRVRLLVLGFAPFILWELFSIIYYGFP
                     FPNTYYAKLNTGISQYTLIKHGALFFFNSIVWDPITLITIGISFLLSMVVGDKPEKMI
                     SLGIGIYLAYVVWIGGDFMTGRFLSVPLLSAVILLVRSLTRFSFAQKTALLAVLILLG
                     LMTDRPSFNAAPAQDLEFETFTGVGDEQAAYYPATGLLREGRVTNVPDHQWYYDGLAL
                     RDQNVNVYEGKGIGFLGFAAGPNVHVIDIFALSDPLLSHLPVQSKTEVLIGHFRRSVP
                     DGYLETLETGRNEIKDPGIAEYYKKLLLITRGKIWSFERWQAIWKLNMGQYDDLLAPY
                     INKLN"
     gene            complement(3630..4790)
                     /locus_tag="VFQ23_04795"
     CDS             complement(3630..4790)
                     /locus_tag="VFQ23_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9905929.1"
                     /translation="MPLEIHSKTVPAPYKSDPRIERDSNGTWHVRGYAEARDLLKEEM
                     IQEGFNAADLRKAGFEAVLYQHGEPHRQSRAAIARFFSPTTVQQSHIPLIESTADEIM
                     ADLKSKKRANLRELSRQMATVVASAVIGLTPTKAMIRRLDIMLHSPAYSGNPIVQWLQ
                     SLRGNYLRFAFFFGDVMPAISRRRKSPQNDVVSHMLSKGKRPLDIWLECVVYAAAGMA
                     TTQEFICIVLLHCLRDEKFRNIMTSDNTSARYEALNEILRLEPVIGKLYRRTHEPVTV
                     TSEGKEVTIPEGEKITFHVYDVNVDSKAVREAPTELNTQRKMEKGVYRSMIGFGSGPH
                     RCAGEHLALAETDIFIHKILQIPGLRIESGPNIMRNDTVEGYEVNDLILVVD"
     gene            5242..7908
                     /gene="gyrA"
                     /locus_tag="VFQ23_04800"
     CDS             5242..7908
                     /gene="gyrA"
                     /locus_tag="VFQ23_04800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET9905930.1"
                     /translation="MELGLIRKIDIDTEMQQAYLDYAMSVIVARALPDARDGLKPVQR
                     RILYAMYDMGIRADSAYKKSARIVGEVLGKYHPHGDSAVYEAMARMAQDFSMRTLLVD
                     GQGNFGSVDGDPPAAMRYTEARLTSAALDILFDLNKDTVDFNPNFDDTLTEPGVLPAA
                     LPNLLVNGATGIAVGMATSIPPHNLSEVIDAIVYMLEKYEKLDDVDVETLMEFVQGPD
                     FPTGGLIVQQKDEDGLEAAYGSGRGRITVQAKAHVEEMERGKNRIIVTELPYMVNKSS
                     LIERIADLAREGYLEGLSDLRDESDRHGMRIVLEMTKNADPEVVLRDLYKRTPMQTTF
                     SINLLALVDGEPRTLTLKQALRVYIEHRLTVIKRRAEFDLEKARQRAHILEGYLVALK
                     NLDEVINIIRAASDVETARGKLMKRFKLTELQATAILDLQLRRLAALERKKIETEYKE
                     VAGHIKELTDLLKSPKKMRGVVADELLNVKAQYGDRRRTQIVDLSANGKGAKTLTARD
                     LLPEQQVWIGVTEDGLVSRTHDDKQPKHSGNDAPRWLVKASTTDTIYFVAKSGRAAAV
                     AAHIIPQAEKLSQGTLFHRVSPLTESDSLAAVFALPSKKSGLPEETCVITTSKLGMIK
                     KSLISELPGPSSQSFVLVRMNEGDRLISVGLTDNKTKDILLVTAQGMAIRFKEDEVRP
                     MGLVAAGVNGLKLADKDEVVGMEILPAEGELFLITSEGKAKRVDQKEFPVQGRYGRGV
                     IAWDLPEKVRLAGAVADKPNHMATIHLTKAAPKSTRLDEAGIRKRAATKGDVIVEVRP
                     GEEVVSINVSWTVEKFVEADSGKKNGRVKASANGKAPVAKTKSAPAKKAKASPPKAKS
                     KTAKKPAATKKSAPKTVSKTAKKPQSSKRKKK"
BASE COUNT         2169 a         1968 c         2062 g         1929 t
ORIGIN      
        1 tactgtatat gccccaccct cgtgtttcaa aaaagcctgt cggacggcta acgcggggta
       61 agacagcttc gaatcgactc cgccgcgttg acaatttcat cctgctctat gagccgtcgc
      121 ttctcccacg gaccgagggc ctgtttgccg attcgttgtt cgttgacctg ggctacggct
      181 tcgatgcgcg gaccacactg gaaagcgcgt tgcggttcag gcgtgtaaac cctggcttga
      241 gggtcctggg tgttgaaatc gacaaagatc gtgtagaggc tgctctgccg tatgcagata
      301 aaaagacatt ttttcgcctg ggtgggttca atcttccttt gagcgatggc gaacatgtcc
      361 gtttgatccg cgccttcaat gtcttacgcc aatatgaaga aaaggatttt gctcctgcct
      421 atgatagact tgcagcgtat gtcctccccc atggattaat gatcgaaggg acgtcgaatc
      481 cgtttggaag tatttgggtt gcaaacctcg taagaaaaga agaatctggt cattggcgtt
      541 ttgaagcgtt ggtattcagt acaaatttcc ggacagggtt cgaggtggaa gaatttcaaa
      601 cgattctgcc gaaaaatttg atccatcatg ttgcacctgg cgaagagatt tatgatttct
      661 tcgaagcgtg gaaacaagct gccaaaggaa cggcttcgat gaaaaactat ggcaccagac
      721 aatggtttat cgctgcggca gaacacctgg caggtcaggg ctatgatatc gaatcccagc
      781 gaaaatggct gtcaaaagga tacttaatct ggaagcaaaa tgaacggaac aaatcgtagg
      841 tattgagcgt cgtcctttga agatgacctg tataggcatc attctttcag tttttaactg
      901 aaaggaggag atatgaaaaa gtccaatcct ttcctttttt tggctgctgt tttgctgatt
      961 gtttcgttgg cctgtgtctt gcccagcacc gtcacgccag acccaaattt tattagtacg
     1021 gcgatcgcgc agacgattgt cgcaggtgtg agtcagaccg gtgcagttcc ctctgatgtg
     1081 cccatcattc tggaatcccc aacgagtaca caaacattta caccagaatt acccaccctt
     1141 tcacccactg tgaccttaac gccatcgcct gtatttaccg ctacgccgct cgtgccgttg
     1201 atcagcgtct cggtggcgac caactgccgt atcggacctg gcaggcaata tgaccgggtg
     1261 ggtgcattgt tggtgggaga ggtcgccgaa gtatatggac gcgatcccac aggtgcctac
     1321 tggtatatcc gcaacccgga tccaggccca gaattctgct ggttgtgggg cgaatatgcc
     1381 actttggtgg ggaataccgg cgccctgcca atttacacgc cgccgccaac acccacaccg
     1441 gctcctaact ttgatccggt ttacctgggc agggatacct gtacaggcgg ctggtggtta
     1501 gaatttgagt taaagaacac tggcggaatc cagttcaaat ccttctcgat aactgtgaga
     1561 gatacagata ccgacatcgt tctttcgctc tacgccgatg gttttacaga tcgggacggc
     1621 tgttctactt cagtcacgag agacaatctc aaccccggtg acagcaggat tgtcagctca
     1681 ccttcctttg gctatgatcc cattggccat gacatccgcg tcacagtcac ggcatgttca
     1741 gggcttggtc aaacagggat atgtgtttca aaggtcatca atttcgatgc ataaaacaaa
     1801 cagggcaggc cttgagcctg ccctgttttc atttataaaa tgggacgtat caataactta
     1861 atttaacttg ttgatatacg gtgccagtaa atcgtcatac tgccccatat ttagtttcca
     1921 aattgcctgc cacctttcga aactccaaat ttttcctctt gtaatcaaca ataatttttt
     1981 gtaatattct gcaatcccag ggtccttgat ttcattcctg cccgtctcga gtgtttccag
     2041 atatccatcc ggaacggacc gcctgaagtg accgatcagc acctctgttt tggattggac
     2101 tggcagatga gaaagcaatg ggtctgaaag agcaaatata tcgatcacat ggacgttggg
     2161 tcccgcggca aaccccagga agccaatccc tttgccttca taaacattta cattctgatc
     2221 gcgcagagca agcccatcgt aataccactg atggtccggg acgttggtta cgcgaccttc
     2281 tcgcagtaac ccggtagccg gatagtaagc cgcctgttca tcgccgacgc cagtaaaggt
     2341 ttcaaattca agatcctgag cgggtgcagc gttgaaactt ggacgatctg tcatcaaccc
     2401 aaggaggata agaacagcaa gaagcgctgt tttttgagca aaagaaaacc tggttaacga
     2461 tcgcaccaga aggatcacag cgcttagcaa aggcaccgat aaaaaacgac ccgtcataaa
     2521 atctccgcca atccaaacta cataagcgag atatatccca atgcccaacg agatcatttt
     2581 ctccggctta tctcccacta ccatggacag tagaaaggag attccgatcg tgattaaagt
     2641 gatcgggtcc caaacaatcg aattgaaaaa gaatagagca ccatgtttga tcagcgtgta
     2701 ttgcgaaatc ccggtgttca gtttggcgta ataagtattt ggaaacggga aaccataata
     2761 aataattgaa aacaattccc acagaataaa gggtgcaaaa cccaaaacga gcaggcgaac
     2821 gcgccttccg gtttctacgg ggactacgag gtcaaggaga gccggcaggc acagcaacaa
     2881 taaatccatg cggttcagcg taagcaggcc ggccagcagc gcaaggatca gaatgcggtt
     2941 ttgcgaacgt tgatcattcc atttcaagta ggccaatgtg aacagcaaaa caagcaagtg
     3001 cgatgccggg ttctccaacc ccgaagttga ataatccagg gaggcattgg aaagcaacaa
     3061 aattacccag ccaataaaaa cactataggt atcttcggat gtatgcttga ggaaaatcca
     3121 tataacaacc agagagacga ataaagacag ccctagcagt gaaatgtatg cgtcccgagt
     3181 tattgcataa acaattgtca gaataaacat ccaaagggga tgagtaaacg tctggaggcg
     3241 ttcaccgaca ttccacgtgg gtccgtatcc attcagcaaa ttgtccaccg tgcgcatggt
     3301 gatatacgcg tcatcgccca cccaggccgt tcgtaggaga acaaatccaa atagcaaaag
     3361 taaagaaatg ttcaggagtt gtctggcgtt gaatttcatg gctgcgggat aagcccaaat
     3421 tttactacgc cagatgaact gagatgggca ggctttgagt ttgagccggg agtggcgaat
     3481 ttagtgtgta agccacagag cccacagagt ttttgagttt ttttccacct gccccgaacg
     3541 caggtgcggg gtcaaagggc tcagtgctct ctgtggctaa gtagatcact cactctctga
     3601 gcccctccct ttgagcctgc ccatcttctt taatccacaa cgaggattag atcgttgacc
     3661 tcatatccct ctactgtgtc gttgcgcatg atattggggc cactttcaat gcgaagcccg
     3721 gggatttgta atattttatg gatgaagata tctgtttcgg caagtgcgag atgttcaccc
     3781 gcacatcggt ggggacctga cccaaacccg atcatcgagc gatagactcc cttttccatt
     3841 ttgcgttgtg tattaagttc ggttggcgct tcgcggactg ccttggaatc tacgttgacg
     3901 tcgtagacat gaaaggtgat tttttcacct tcaggaatgg tgacttcctt tccttcgctg
     3961 gtcactgtca caggctcgtg agttcggcga tataactttc cgatgacagg ttcgagacgc
     4021 aggatctcgt tcagcgcttc ataacgagca ctcgtattat cagacgtcat aatgttgcgg
     4081 aatttctcat ctcgcagaca atgcagtaaa acaatacaaa tgaattcctg ggtggtcgcc
     4141 atgcctgctg ctgcatagac aacacactcc agccagatat ccagtggacg tttccctttg
     4201 gagagcatat gtgacaccac atcgttttgc ggcgacttgc gccggcgtga aatggctggc
     4261 atcacatcac cgaagaaaaa tgcgaagcgg agataatttc ctctcaggct ttgcaaccat
     4321 tgcacaatag ggttacctga ataggctggg gaatgcaaca tgatatccag gcggcggatc
     4381 attgcctttg tgggggtcag gccgatcacc gcggaggcaa cgacggtggc catttgcctg
     4441 cttaattctc tgagatttgc ccgttttttt gatttcaaat cagccatgat ctcatccgcc
     4501 gtgctctcga tgaggggaat atgagattgc tgaaccgtgg tggggctgaa gaatctggcg
     4561 atggccgcgc gagactgccg atgaggttca ccatgttgat aaagtaccgc ctcgaaaccc
     4621 gctttacgaa gatctgccgc gttaaaccct tcctggatca tttcctcctt gaggagatcg
     4681 cgagcttccg cgtagccgcg tacgtgccag gttccgttag agtccctttc gatacgcggg
     4741 tcgcttttgt aaggggccgg gactgttttt gaatgaattt ccaaaggcat ttaaagtttt
     4801 ctcctgatct agaattcgcc aattatacat agaggatggg ctgaaatgac ctttcagatg
     4861 attaaccatt aagcatctct tatggaaaag ccatttctac agaggcttaa gtgaacaaag
     4921 actgaacaag cttttcctgc ctcgtttgtt atgggaaaaa agcgaaaaag acagaatcat
     4981 ttacaaagtg ccgaataacc atctggactc agaatttgaa tttagcctgc aacgcaattc
     5041 aaaagtttgc tcattaaata atacaagcgg caagaacact tgacgccttt cctctttcgc
     5101 cgtaagatta tcagacgata tagaacatta attcactcaa cgaagctttg ccgcgaagat
     5161 acttttattc ctatggggat accctctcac tttcaatctt cgtgccctta agattctttg
     5221 gtggatggaa aaggatttca catggaactt ggtttaattc gaaaaataga tattgatact
     5281 gaaatgcagc aggcctatct ggattacgcg atgtctgtca ttgtggcgcg cgctttgccc
     5341 gatgcacgcg atgggctcaa acctgtgcaa cgccgcatct tgtatgcaat gtacgacatg
     5401 ggcatccgcg ctgactcggc ctataagaaa tctgctcgta tcgtgggcga agtactgggt
     5461 aaatatcatc cgcatggtga ctcagcggtc tatgaagcca tggcgcgtat ggcgcaggat
     5521 ttctccatgc gtaccctgct tgtggatgga caaggcaact tcggttctgt ggatggcgac
     5581 ccgccggctg cgatgcgtta caccgaagcg cggcttacct ctgcggcact ggatattcta
     5641 tttgacctca acaaagacac agttgacttc aaccccaact ttgatgacac attgaccgag
     5701 ccgggcgtcc tgccggcggc tttgccaaat cttttggtca acggtgcaac gggtattgcg
     5761 gtgggtatgg caacctcgat tcctccgcac aacctcagtg aagtcattga tgccatcgtc
     5821 tacatgctgg agaaatacga aaagcttgat gatgtggacg ttgaaacatt gatggagttc
     5881 gtacagggac ctgatttccc caccggcggg ctgatcgtac agcaaaagga cgaagatgga
     5941 ctcgaagccg cgtacggttc agggcggggt cgcataacgg tgcaggccaa ggcgcacgtc
     6001 gaggaaatgg aacgcggcaa gaaccgcatc atcgtcactg aactgcctta catggtgaac
     6061 aaatccagcc tgatcgagcg catcgcggat ctggcgcgtg aaggctacct cgaagggctt
     6121 tcagatctcc gagatgagtc ggaccgtcat gggatgcgca tcgtgctgga aatgacgaag
     6181 aatgccgatc ccgaagttgt gctgcgcgac ctctacaaac gcacacctat gcagaccacg
     6241 ttcagcatca atctgctggc acttgtggat ggcgaacccc gcacgttaac gctcaagcag
     6301 gcactgcgcg tttatatcga gcatcggctg acggtcataa aaagacgcgc ggaattcgat
     6361 ctggaaaaag caagacagcg cgcacatatc cttgaaggct atctcgttgc tctaaaaaat
     6421 ctggatgaag tgatcaacat catccgcgca gcctcggacg ttgagacggc gcgcggcaaa
     6481 ttgatgaaac gcttcaagct gacagagctc caggcgaccg ccatcctgga tttgcaactg
     6541 cgccggctcg ccgcgctcga acgcaagaag atcgagaccg aatacaagga ggtagcgggg
     6601 catatcaagg aattgactga cctgttgaag tcgcccaaga aaatgcgcgg cgttgtagca
     6661 gatgaattat tgaatgtaaa agcgcaatac ggtgaccggc gccgcacgca gatcgtggac
     6721 ctctccgcaa atggcaaggg tgcaaaaact cttacagcga gagatctcct gcccgaacaa
     6781 caagtctgga tcggtgtgac ggaagacgga ctggtctctc ggacgcatga tgataagcag
     6841 cccaaacatt ccggcaatga cgcgccgcgc tggttagtca aagcctccac cacagacact
     6901 atttactttg tcgcgaaatc aggtcgtgcg gcagcggtag ccgcgcacat catcccgcaa
     6961 gctgaaaagc tatcgcaagg cacgttgttc cacagggtgt caccgcttac cgaaagcgat
     7021 tcgctggcgg cggtgtttgc gttaccctcc aaaaaatccg ggttgcccga agagacctgt
     7081 gtgatcacta cgtcgaagct gggcatgatc aaaaagagtc tgatcagtga acttcccggg
     7141 ccttcttcgc aatcctttgt gctggtacgg atgaatgaag gagacaggct gatttctgtc
     7201 ggtctcacgg ataataagac caaggatatt ctcctggtga cggcacaggg aatggcgatc
     7261 cgcttcaagg aagatgaagt tcgccccatg gggctggtcg ctgctggtgt gaacggcttg
     7321 aaactggctg ataaggatga agtggtcggc atggaaatcc tgcctgcaga aggcgagctc
     7381 tttctcatca caagcgaagg caaagccaag cgagtggacc aaaaggaatt ccccgtgcaa
     7441 gggcgctatg gccgtggtgt catcgcctgg gacttgccag agaaagttcg attggctggc
     7501 gcggtggccg ataagccaaa tcatatggcg acgatccatc tgaccaaagc tgcgccaaaa
     7561 tccactcgcc tggacgaagc aggcattcgc aaacgcgccg caaccaaggg tgacgtgatt
     7621 gtggaagtga ggcccggcga ggaagtggtc tccatcaatg tgagctggac ggtggagaag
     7681 tttgtagaag ctgacagtgg aaaaaaaaac ggtagggtaa aagcttccgc gaatggcaag
     7741 gcgccagttg cgaaaacgaa gagtgccccg gcgaaaaaag caaaagcatc gccacctaaa
     7801 gcgaaaagca aaactgcgaa gaagcctgct gccacaaaaa aatctgctcc taaaacggtt
     7861 tctaaaacgg cgaagaaacc acaatcttca aagcgtaaaa agaagtaaac cgaaagctat
     7921 acagaaagtc gacgcagata tgaaagggat atatacgaaa aagtaggctt ctaaatttgc
     7981 aaagaaaacg aaataataca tttaggcgca acaagataaa atgagtttgt tgcgcctttt
     8041 tgttattcat ggagagttat catgaccacc acactattat gggataagtt tgcgacaatt
     8101 gaaaatttta cattggcttg gcagcgca
//
LOCUS       DASTJP010000158        20563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_348445, whole genome shotgun sequence.
ACCESSION   DASTJP010000158 DASTJP010000000
VERSION     DASTJP010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20563
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348445"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VFQ23_04805"
     CDS             complement(<1..778)
                     /locus_tag="VFQ23_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905931.1"
                     /translation="MSKHRIGLLFLILALLSTFRIAPAQAQSGGWSEPYRLSSEDGSA
                     SEGYLVADQYGYVHCFWTEALSESQRNVIKYARFDGSTWTKPNDIYVTGLELRNISPF
                     VDQHGMLYIAWAEGQSGSTYYHYMYTYAPAINALSAQSWAPPLQTVLPARTAYMRVDT
                     EGIFHILYIDQTEEAGVYYMRSSDMGATWSEPVWLDPDIPPDHIPDSLNFELDDQNGL
                     HAVWWYGGLNRARPDWVRYIHSLDGGGTWSAPFTLDQYNEE"
     gene            complement(848..1465)
                     /locus_tag="VFQ23_04810"
     CDS             complement(848..1465)
                     /locus_tag="VFQ23_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905932.1"
                     /translation="MKSIYSRFFEKKEQQQFITIVSGLPRSGTSLMMSMLAAGGLEAV
                     TDQLRVADDDNPVGYFELENVKKLISGEHSWLANSNGKVVKVISTLLPYLPDGYHYRI
                     IFMRRAIEEVLASQRKMLINRGENPNKITDAQMADVFKMDLQQSERWINSQINVTRMD
                     IQYKQLINHPSPLVTEINKFLGGDLDQNKMLGVIDPSLYRQRSSN"
     gene            complement(1486..2862)
                     /locus_tag="VFQ23_04815"
     CDS             complement(1486..2862)
                     /locus_tag="VFQ23_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9905933.1"
                     /translation="MNRPNVVLLTIDTLRADRLGCYGNNGLLSPNIDQLANSGIRFEQ
                     AISGGSWTQAAFPVIFTSSYAAMYGGCLGRLSPERPSPIEALASHGYLTSGFTTNLHL
                     SRATGFDRGFRHFVELFPSEIDPKLRRIRGGQRLLQNELTHSMLRPLGIRMRPARLYS
                     SAAEVTNRVCEWLNQADAPFLAWAHYMDLHWPYHMEEDLVNPKDIAQAWQDLADMYGR
                     ANFYGRSDRDKPITSEQREHFIGLYEKSLKYLDNQIGRLLNYIQNSPHADNTIILLVA
                     DHGEEFLDHGRWGHWESNLFDEILRVPLIMWIPNGPHGQVVRQQVRLLDLMPTILDLC
                     GYSTDALLGTSIAPLLSQNESKYDAKEVISEMRRDPWHRVAVRTESFKYIWDSKKPDQ
                     PELYNLRVDPTEKQNVAQQFPQEVKQFQSSVDTHRLRVSETEPATAVPRVKVDEDVAR
                     RLRDLGYL"
     gene            complement(2990..4297)
                     /locus_tag="VFQ23_04820"
     CDS             complement(2990..4297)
                     /locus_tag="VFQ23_04820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9905934.1"
                     /translation="MKIAHIYQESNIRFSEPQAAQLHIYHTIHGLQQAGHQVSLLALQ
                     GRQVLFTEDLEVFKSDKRLASHYGQLGLSGTAHFKGMESGVRRLQSELRFPYLALFDS
                     YRMADAGRKNLRGFDLIHERFNLLSLGGAWATKKLGIPLVLEVNADLLEQRKFKGIPE
                     KGLRRLFAEWSTQMCFKAATKIVCISTGLKDHLSKKWNIDERNLTVLPCAADVDAFGP
                     HHNSGLVRRSLGLANEPVVLWVGGFYPWHNLDLLLGSFALVLQRFPAAKLVLVGEGQT
                     RSTVAEKIKRDGLEKSVIMTGAIPHAQVPEMVSIADVAVVPSAAVSAGGGGTGTPLKL
                     FEYMAAGKAIVATALNEVAEVIHDNQNGLLVQAGDPNQFAASIMKLIDDPRERNRLGQ
                     NARAQAIKQYSWEHYTKRLQEIYFGVVGNTPARSRVVDISGRA"
     gene            complement(4331..5743)
                     /locus_tag="VFQ23_04825"
     CDS             complement(4331..5743)
                     /locus_tag="VFQ23_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905935.1"
                     /translation="MTYKWFKATSFKKLGVSVALFILLAACSSSLTSTPSSVPPHDHV
                     TPVATDALDETTTPKKTKMPPVQQPGQQSVQGLEQNGIPPYPDAPLCADSGESHDNSL
                     FHTLWDGTRGCHYDHEHGQNPFTPEVAAVFPGLNLKALMGDVGIGHTNPSSPKENTQK
                     HGGFKWQVLLQHPNGCEGYQGSELGVDASVVQYHNMGDYSLEFEARVHSALAVLRQCR
                     TDNPTDYGYVYTVQHVDFGQRVVPYQGMVFPYPDAPNPVYDPGLSPYFTLDCIGNGQQ
                     CRPSREYVLDRNANANSVWTSQPNKLGESGSNLFQLLFRVRDNYQLLDYADLTYPFTF
                     VWLCSNDNGLTFSPEPGCRYNNSTGRIHEIYGVIPTEWDNLEGLDIDPRVGRITGEGF
                     VTAIGDLNPTCTNPGHDCHPIKMVEAFTGYYGSHLIAGKADQFSPESQPERDIYFCGT
                     QVCSENDPGAMPSGWIGQNN"
     gene            complement(5740..7044)
                     /locus_tag="VFQ23_04830"
     CDS             complement(5740..7044)
                     /locus_tag="VFQ23_04830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9905936.1"
                     /translation="MNPLKIHRKQSFYLGIILIIFGVLLKRLIESTLISDGQIELPLY
                     NALIFVFQLLAVAAGFFLLIRQPEVKLPKRTDVVLVLFSTFLTFFLLEMMSRVWLNYL
                     ATPEQYDRFVLFTSIDPKEFTFAPHPYLSYYPTPNYKKGLTSHNSLGYRSEEFPLEKP
                     NGVYRIVALGGSSTYDVSIKDNADTFTAQLEKMLKDDYGYQNVQVINAGVPGYNSWEI
                     LVNLEFRVLDLDPDLVIIYEGTNDVHARMVEPASYRGDDLGRRQAWQVPAVALWEHSA
                     ALRITSRMLSFTRQVSIDDFVSSPTYVSWPFEYRLIEDNLDPNEILKENPPIYFRRNL
                     ENMTAVAKAHNITILLSTWAHSPFLNDYAAEDYYQLAFAENNDVVKEVATEQQIPLFD
                     FAAVMPKDARYWADGRHVNEAGAVEKARLFAEYISEQGLIDE"
     gene            complement(7064..8326)
                     /locus_tag="VFQ23_04835"
     CDS             complement(7064..8326)
                     /locus_tag="VFQ23_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9905937.1"
                     /translation="MNKIAIEVAGLGKEYHIGGPQKTYHRLTDQLADMALTPLRRAGK
                     LLRGQANGAAELDETIWAVKDVSFQIKPGEVVGMIGRNGAGKSTLLKILSGITEPTRG
                     YADLYGRIGSLLEVGTGFHPELTGRENISLYGAILGMKKAEIERKLDEIVAFAEIDKF
                     LHTPVKHYSSGMYVRLAFSVAAHLEPEILLVDEVLAVGDMAFQRKCLGKMDDVAQEGR
                     TVVFVSHNMGLLQQLCERGIFLQHGTVHTDGTITEAVDAYLQTLEQAEALDLSERTDR
                     KGQGKVHLIGAEVTNGSSSILRTGHPARFTFQIDKLVPGIACNFNVYDTIGQPVTTFQ
                     SRVRAPEDSSDPENGMKFICDLDELLLLPGRYRVDVAIIGDNRLQDFLEAATVFEVAE
                     GHVRGRPVRPDGKFSVSMSHRWILPTDK"
     gene            complement(8336..9184)
                     /locus_tag="VFQ23_04840"
     CDS             complement(8336..9184)
                     /locus_tag="VFQ23_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9905938.1"
                     /translation="MNETTLTSSDLPRTRRQPSSGWALPKFGELWEYRELLFFFVWRD
                     IKVRYKQTVLGAAWAIIQPFFTMVIFSLFFGRLASIPSDGLPYPIFSYAALVPWTFFA
                     TALTQASNSLVMSANMVKKIYFPRLTLPIATVLAGVVDFGLAFLVLMGMMFFYGRTPT
                     INILWLPLFLLLALITSLGVSLWLSAMNVQFRDVHYTIPFLTQAWLFITPIAYPSSLL
                     PEPWRTLYGLNPMAGVVEGFRWALLGTDTAPGPMTLVSAVVALVLFVGGAFYFRRMEQ
                     NFADVL"
     gene            complement(9197..9877)
                     /locus_tag="VFQ23_04845"
     CDS             complement(9197..9877)
                     /locus_tag="VFQ23_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET9905939.1"
                     /translation="MSRVIVFDLVGTLLDLRTMDQHFERFFGDASVRKEWFMQTIQLA
                     LATMLTNTYEDFGVQADTALEITARHYKVSMLGEEKNLILGTLRKLRPYPDVAPSLQR
                     LRDAGLRLAALTNSTVAAAEAQLEFAGLSNHFEQVIYADEVQSFKPAPSIYHHAASRL
                     GVEPKEMRLVSAHHWDVTGALQAGCAAAFVARPGQVMNPFGPQPDMKASDLSELIDKI
                     LEVELAPG"
     gene            complement(9918..11234)
                     /locus_tag="VFQ23_04850"
     CDS             complement(9918..11234)
                     /locus_tag="VFQ23_04850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9905940.1"
                     /translation="MYDVIIIGGGPAGSTLGSYLSKAGIKNLILEGHNHPRPHVGESL
                     VLSTMRVFGELGLIDKMDQAGFPKKFGASWRDFNGKEQALHFAEFRQEGIDRDHTYHV
                     DRSKFDLLLLKHAESLGSEVHQGVQVRRVNFEGDQAVGVRARFAGQEIDIPAKIVVDA
                     SGRQTLLGRQLNLKQNDKIFNQYAVHGWFENVARGEDPKTEDYIHIYFLPVKRGWAWQ
                     IPINDKITSVGVVAEREVFQQFKSESERYFNTYVESNKGLAKAMANAVRVNEFKTEGD
                     YSYILDKFCGNGFLMVGDAARFVDPIFSSGVSVAMHSARFAAQTIQAALESGDLSEAS
                     FKPYEETLRGGVDIWYEFIRLYYKLLPLFTLFIQSEYRVEVLRLLQGEVFNREDVKVL
                     NVMRRYIEAVENNERHVFRGQLTDVPIDDILKQVDKEQNTQQGVEA"
     gene            complement(11313..12764)
                     /locus_tag="VFQ23_04855"
     CDS             complement(11313..12764)
                     /locus_tag="VFQ23_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9905941.1"
                     /translation="MNRNLVFYTLTIAIFGLLIWFVLNQGVRLENPQTIDMVRSEQNS
                     VTAPESALRVVVRGFAQNTAHPLSRLLLQIAVIVVASRSLAFLVAKIGQPRVIGEIVA
                     GIVLGPSLLGLFWPEVTNFLFPPDSLPNIEILSQLGLILFMFIIGMELDVHVLKHKAH
                     TALLVSYSSIIFPFFGGLSLAYFLYKTLAPPQISFLSFGLFMGISMSITALPVLARII
                     QERGLTQTPLGVLAITCAAANDVTAWCILAVVIAIAQAGVVTSALFTILLSVLYVFAM
                     LVLVRPILKHLSVRYGSGENPNHSFTIVAFVVLFLSSFATEVIGIHALFGAFLAGVVM
                     PVSVGFRKMLAAKIEDVSLVILLPLFFASTGLKTQIGLLNDGSAWLICGLIILVAVAG
                     KVGGSLVAARFTGQSWSESLALGTLMNARGLMELIILSIGYDLGVLSPTVFTMMVLMA
                     LVTTFMTSPALSAIQQFNSKNALTGVNPVAERL"
     gene            complement(12766..13911)
                     /locus_tag="VFQ23_04860"
     CDS             complement(12766..13911)
                     /locus_tag="VFQ23_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9905942.1"
                     /translation="MDFSWTKEQLDFKNTVIEFAKKELNEGLIDRDRQGELARENWIK
                     CAKLGILGLGIPEAYGGSATDIMTTMLVMEGLGYGCRDNGLIFAMNAQMWSVQHPILT
                     FGTEAQKQKYLPGMCSGELIGAHGMSEPDSGSDAYRLRARAERTSDGYILNGTKMFVT
                     NAPVADMALVFATVNPEKGIGGVTAFLIDKGTPGFRVSKNIEKMGLRTSPMGELILED
                     CILPVENRLGAEGAGSSIFNSSMEWERSCILGSHIGAMERQLEAGIRYARERRQFGQP
                     IGKFQSVANRIADMKVRLETARLLLYKVAWLNQNGKPAIMEAALAKLYLSECFVDSSM
                     DAIRTYGGYGYMTEMEVERDLRDAVGGTLYSGTSDIQRMIIARWLGL"
     gene            complement(13953..15074)
                     /locus_tag="VFQ23_04865"
     CDS             complement(13953..15074)
                     /locus_tag="VFQ23_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9905943.1"
                     /translation="MSSLTTSSDLKTPALSKRAERLLTVILAVVSPLLLIAMLEGAAY
                     FWERKQANSLYAWELVASRRMVWVQHPEPGAGYTLMQPNSHYEWQGISVDINSHGLRG
                     PETTYQKPSETIRILNLGDSVVMGWGVSEENTYGQQLEALLNEDGISKSNFEVINAGV
                     PGWSLDNALAFFQAEGMKYESDVVVLDLTIANDINGKSALLAGNDPQPLRWLNEHTYF
                     WPFLQNQLSWTKARAEGRDRVDTIDPPTNAAKYFPLDPQSERWTERWDTILEINRLAK
                     ENNIPIVLVMFPLEFQVIDESYSTLPQELFAAKAEEAGIPVLDLLPVFRQACLQKPGG
                     ECQLEDRYLFADVWMHPSAEGNKLTANELAEFLPGIIKH"
     gene            complement(15089..16354)
                     /locus_tag="VFQ23_04870"
     CDS             complement(15089..16354)
                     /locus_tag="VFQ23_04870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9905944.1"
                     /translation="MLKSDRNRQVLTLILIVAALIRIPVAAYMGDQVTELPGIQDQIS
                     YDALARSLLDGRGYSFREAWYPFTPANTPTSHWSFIYPVYLAGIYAISGYHPLIARIL
                     QTVVGSVLTCFLIYKIGRRVVNEATGLAAAALAAVYGYFIYYNAALMTETFFMVLVLL
                     TLYLSIEIKENPTLIRWVGLGCSLGVAVLLRQTVLIFAPILLLWLFAELKDKSSRWWH
                     FSIPIIIIILFVMPWTIRNYRVYDQFLLLNSNAGYALFASNNPGLGTNWDNELVVVPV
                     PQELKGQNEAQLDRALTRKGIEYIISDPERYVFLTLNKTLEYFWFWPSATSSRISNIN
                     RVLSFGLYLPFMLVGLYLSFSRWRRFVVLYLFILVHTTIHLLSWPAPRYRLTVDAVAM
                     VFAAQAVLELVKWAKNWRHRNLISDEARA"
     gene            complement(16341..17387)
                     /locus_tag="VFQ23_04875"
     CDS             complement(16341..17387)
                     /locus_tag="VFQ23_04875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9905945.1"
                     /translation="MKVLCLLDNEVKQGDRWLWKYLPSNNDEIDFLVATGAVDRFQKW
                     GKLLNYYPTYFQAGFQALRKTRQTHYDLVVAWEGKNGFPYALIRSLVGQNHPPLIILA
                     FNIRGVISHFRSLARFGLRSVSRVVVFTPGEVEQYQQLLALPPNKISYAPHGWYDPMS
                     WYDPESVNKSESAARAGKFILASGRSYRDYGTLASAVEGTDVNVKVSGRAFNLAGIDL
                     PINMEATGWLAEREFQDYLYSSSFYVVPLQPITFAGGDSSVLQAMSFGRAVVATRAPS
                     TETYVEHGQTGLLVEPGDIQGMREAILQLWQNPDEAARMGREARLRFEENHTIDKLAQ
                     RVYDVALEVCHAQE"
     gene            complement(17406..18629)
                     /locus_tag="VFQ23_04880"
     CDS             complement(17406..18629)
                     /locus_tag="VFQ23_04880"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905946.1"
                     /translation="MNEQQVIKRAFVPVLGNVFLVLLGVVISLAMVEMLMRAFPNWIP
                     PDVRVNPPARRVKAFIDESYYLRQSDGDLFYYMRGQIAPLSPDEDEVVAQVQMVTDAN
                     GFRNSPEEQATYDIVALGDSFTRASGVAYSWPQKLAEYAKRDVLNLGDVGFGPQDELK
                     VLQQYGIKKQPQWVIMAYFEGNDLYDSAAYEQANPFILFRFGKYALNQSIKSWNEKSW
                     GVAPVKVRSNYQYPINIAINNKDLEVAFFSYYFSWLSVDRAVIESSQNFRLVSETILQ
                     VLELSEAAGARFLLVYVPSKEHVYLPYLNDENILERVFTGVPAVELDEAGFLQFTNER
                     VKPEPTRRHMDDQAGLLADFAADHMILYLDLTSTFQEEAAAGAELYYRFDTHWNQHGH
                     DLAASLINKYIEKIH"
     gene            complement(18626..>20563)
                     /locus_tag="VFQ23_04885"
     CDS             complement(18626..>20563)
                     /locus_tag="VFQ23_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905947.1"
                     /translation="EEQHLIYQRGLQWIFRGAIQLILYRYINYYIMIAPGDVTNTLEL
                     VRYLISNFALYLRISGQFHVIIGILHLFGFNLPETHHLYFMASSFTDLWRRINIYWKD
                     FMLKVFYYPTYFKIRQWGPNSSLVFATAFVFFLTWFFHAYQWFWLRGTFLFTAPDMVF
                     WFILACLVIVNTLYENRRGRKRALGRSSMSVGDLTSLALRTAGTFTVMAVLWSLWSSD
                     SIQDWIALISVVEFTPENIALLLLVFLMLSVIFGLTLWISSRSNDNTERKSKQHNFRR
                     TTAATASGIVLLFLIGNPVVYSQFGGRPQELISDLTVDRLSDREAALLQRGYYEDLIG
                     VNRFNSELWEIYTKRPSDWVALRDTAAIQLTNDDLILELAPSTKIDFNGTELSINRWG
                     MRDRDYEQIPAPDTYRIALTGPSFVMGYGVADDEGFEPLLEDRLNHENSGNAYTKYEI
                     LNFAVPGYSAFQNLLVLEQKALQFQPNAIFVMAHQREEDAMVLYLADRLTTGVEFPYA
                     DLIELAHRAGVEAGMTKAEAERLLKPYGSEILLWTYRQIVEVSHSHGILPVWIFMPTL
                     EDPLHEEEIIYLTGLAEESGFIVLDLSDAYDNQDLDSLVVAYWDKHPNAKGHMLIAED
                     LYRKLREKEEEIPLFQSSASR"
BASE COUNT         5437 a         5149 c         4841 g         5136 t
ORIGIN      
        1 gctcctcgtt atactgatca agcgtaaaag gggcggacca ggtgccgcct ccatcgagag
       61 aatgaatata gcgaacccaa tcaggccgcg cccgatttag tcccccatac caccagacgg
      121 catgtagccc attctgatca tcaagctcaa aattcagact atcaggaata tgatcgggcg
      181 gaatatccgg atctaaccat acgggctccg accaggttgc gcccatatcc gacgagcgca
      241 tataataaac tccagcttct tctgtttgat caatatatag tatatggaaa atgccctctg
      301 tatccactcg catgtaggca gttctggctg gtaagacggt ttggagggga ggtgcccagc
      361 tttgcgctga taatgcgtta atggcgggcg cataggtgta catatagtgg taataagtag
      421 aaccactttg gccttcggcc caggctatat acaacatgcc gtgctgatct acaaaaggcg
      481 atatattcct aagttcgagt cctgtaacat aaatatcatt tggtttagtc catgttgaac
      541 catcaaaccg tgcatatttg atgacattgc gctggctttc ggaaagtgcc tctgtccaaa
      601 agcaatgcac atagccatat tggtcagcca ccagataacc ctcactggct gacccgtcct
      661 cacttgacag gcgatacggt tcagaccatc cgccagactg cgcttgtgct ggcgcgatcc
      721 gaaatgtgga tagcagtgct agtattagaa ataaaagacc aattcggtgt ttgctcacga
      781 ctcaagctct gatgatagta tgcgctgcgc aggcggttgc caaactgaaa cacagctcca
      841 tgccacgtta gttcgacgac cgctggcgat ataaagaggg atcgattacg cccagcattt
      901 tgttctgatc caaatcacca cccagaaatt tgttgatctc ggtgaccaaa ggactggggt
      961 gattgatgag ctgcttatat tggatatcca tccgggtgac attgatttga gaattgatcc
     1021 agcgctcact ctgctgtaaa tccattttaa aaacatctgc catttgagcg tcagtaatct
     1081 tatttggatt ttcgccgcga ttgatcaaca tcttgcgttg tgaggcaagt acttcttcaa
     1141 ttgcacgtcg cataaaaatg atcctgtagt gataaccatc gggaaggtat ggtagcaagg
     1201 tcgatattac cttgaccact tttccgttcg agttcgccag ccaggaatgt tcccctgaga
     1261 tgagtttctt gacattctca agttcgaaat agccaacggg attgtcatca tctgcgaccc
     1321 taagttgatc tgttacagct tccaacccgc ccgcagcaag catactcatc atgagcgagg
     1381 tccccgaacg cggtaacccc gacacgatgg taatgaattg ctgctgctcc ttcttttcaa
     1441 aaaaacgcga ataaatactt ttcattaata cctgtcacca gattactata aataacccaa
     1501 gtctcgcaga cgcctggcga catcctcgtc caccttaact cttgggacgg ctgttgccgg
     1561 ttcggtctca gacacccgga gccgatgtgt gtctacactg gactggaact gtttgacttc
     1621 ttgcggaaat tgttgagcca cattttgttt ttcagtggga tccacgcgca gattgtataa
     1681 ctcgggctga tccggtttct tgctgtccca gatgtactta aacgactcgg ttcgcactgc
     1741 cacacggtgc catgggtcgc gtcgcatctc gctgatgacc tccttggcgt catatttaga
     1801 ttcattttgt gagaggagag gcgctatgct cgtccccaaa agagcatctg tggaatagcc
     1861 gcaaagatca aggattgttg gcataagatc taataaccgt acctgctgcc ggacgacctg
     1921 gccatgaggc ccattcggaa tccacataat gagcggcact ctgaggattt cgtcaaacaa
     1981 gttgctctcc caatgacccc agcggccgtg atcgaggaac tcttctccat gatcagccac
     2041 gagaagtatg atggtattgt cagcatgagg tgaattctga atgtaattga gcagtcgtcc
     2101 aatttgattg tctagatatt tcaatgattt ctcatacaaa ccaatgaaat gttcgcgctg
     2161 ttcacttgta atgggtttat ctcgatctga acgaccgtaa aagtttgccc ggccatacat
     2221 atctgccaga tcctgccatg cctgggcgat gtctttaggg tttaccaaat cttcttccat
     2281 atgatatggc cagtgtaaat ccatatagtg tgcccaggcg aggaagggag catccgcttg
     2341 attcaaccat tcacaaacac ggtttgttac ctctgcagcc gagctataaa gacgcgcagg
     2401 ccgcatacga atacctaaag gacgcagcat ggaatgagtc agttcgttct gcagaaggcg
     2461 ctggcctcct cttattcggc gcaacttagg gtcaatttca ctgggaaata attcaacgaa
     2521 atgacggaat cccctgtcga agcccgtggc gcggctgaga tggaggttcg tggtaaagcc
     2581 actagttaga tagccgtgtg aggcaagtgc ctcaataggc gatgggcgtt caggagagag
     2641 acgcccaagg cagccaccgt acatcgctgc atagcttgag gtaaagatga caggaaaagc
     2701 ggcctgggtc caggatccac ctgaaatcgc ctgttcgaag cgaatacccg aattggcgag
     2761 ttggtcaata tttgggctta gaagaccatt atttccataa cagcccagtc ggtcggcgcg
     2821 taacgtgtcg attgtcagta agactacatt cggtctattc atcggttagg gttacctgca
     2881 ggattgagaa ttacatatat ctagacccga ttcgtgaata aaggtaacag gtcgggttaa
     2941 acgagtgcca ggggtagtgt aaatttcggt gatactcatt aacctggaat taggctcttc
     3001 cagaaatatc cacaactcga gatctggctg gcgtgtttcc cactacaccg aaatagattt
     3061 cctgaagtct cttagtatag tgttcccagg aatattgctt gatggcctga gcccgggcgt
     3121 tttggcccag gcgattccgc tccctgggat catcaatcaa tttcattatg gatgcggcga
     3181 actgattggg atcacctgct tgtaccaaca atccattttg attatcatga atcacctctg
     3241 cgacttcgtt aagtgcggtg gctacaatgg ccttcccggc ggccatatac tcaaataatt
     3301 ttagcggcgt acctgtacca ccaccgccgg cggagacagc cgcagagggg actacggcca
     3361 catctgcgat agaaaccatc tctggcacct gcgcatgggg aatggcgcct gtcatgataa
     3421 cggatttctc gagcccatct ctctttatct tttctgcaac cgttgatcgg gtctgaccct
     3481 cacccaccag gaccagtttc gcagcaggaa atctttgcag gactagagca aagcttccta
     3541 aaaggagatc aagattatgc cacggataga atcctcctac ccataataca acaggttcgt
     3601 tggctaaacc cagacttcga cggacaagcc ctgagttgtg gtgagggcca aaagcatcta
     3661 catctgccgc gcacggcagt acagttaggt tacgttcatc gatgttccac tttttgctca
     3721 aatggtcttt gagtcccgta gatatgcaga cgatcttcgt agcagcttta aagcacatct
     3781 gagtggacca ttcggcgaat agacgacgca atcctttttc tggaatgcct ttgaacttac
     3841 gctgctcgag cagatcagcg ttgacttcaa gcaccaacgg tataccaagt tttttcgttg
     3901 cccacgctcc gcccagagac agcaggttga accgttcgtg gatcaggtca aaacctctta
     3961 aatttttgcg gcccgcgtct gccatacgat agctatcaaa tagcgccaaa tatgggaagc
     4021 gcaattctga ttgaagacga cgaacaccgc tctccatacc cttaaagtgg gctgttccac
     4081 ttaatcctaa ttgaccataa tgacttgcca ggcgtttatc gctcttgaat acctcaagat
     4141 cctctgtaaa taggacttgc ctgccttgca atgccaacag tgacacttgg tgaccagcct
     4201 gttgtaaccc gtggatggtg tgataaatgt gaagttgtgc tgcctggggc tcagaaaacc
     4261 ggatgttgct ttcctgataa atatgtgcaa ttttcatcgt aaagaagaaa ccatgggttt
     4321 cgcgccactc ttaattattt tgcccgatcc aaccagatgg cattgcgcca gggtcgtttt
     4381 ccgaacagac ttgtgttcca caaaaataga tgtcacgctc aggctgtgat tccggactga
     4441 attgatctgc tttgcctgca atcaggtgtg agccgtagta gcctgtaaac gcttcgacca
     4501 tcttaatcgg atggcagtca tggccaggat tggtgcatgt agggttcaag tcgccaattg
     4561 cggtaacgaa tccctcgcct gtaatgcgac caacacgtgg gtcgatgtcc aatccctcca
     4621 aattatccca ctccgtagga atgacgccat aaatttcgtg gatccggccg gtgctgttgt
     4681 tatagcggca tccaggctca gggctaaatg tcaacccatt atcgttagag cacaaccaga
     4741 caaaggtaaa aggatacgtt agatctgcgt aatcaagcaa ctgatagtta tcgcgaaccc
     4801 ggaataacag ttggaacaag tttgagccag attcgcctag tttgtttggt tgagaagtcc
     4861 aaacactatt agcattggca ttgcgatcca acacatactc cctgctgggt cgacactgct
     4921 gcccgttgcc aatacaatcc agtgtgaagt aagggctcaa accaggatca taaaccggat
     4981 tcggcgcatc tgggtaggga aataccatgc cctggtaagg cacaacccgc tggccaaaat
     5041 ccacgtgttg gaccgtatac acataaccgt aatcggtggg gttgtctgtt cgacattggc
     5101 gcaagactgc cagtgctgag tgcacgcgtg cctcaaactc caacgaatag tctcccatat
     5161 tatggtattg aacgactgag gcgtctacac caagctcaga gccctgataa ccctcgcatc
     5221 catttggatg ctgcagcaac acctgccact tgaagccacc gtgcttttgc gtgttttctt
     5281 tcggggagga cgggttggta tgcccaatcc ctacgtctcc cattagcgct ttcaaattga
     5341 gtcccgggaa gacagcagcc acttccggtg taaatggatt ttggccgtgc tcatggtcat
     5401 agtggcagcc acgggtgcca tcccacagtg tatggaacaa gctattgtca tgggactctc
     5461 cagagtccgc gcagagtggc gcgtctgggt agggtggaat accgttttgc tcaagccctt
     5521 gtacggattg ttggcccggc tgttgcacag gcggcatttt ggtctttttg ggtgtggtgg
     5581 tttcgtcgag tgcgtcagta gccactggag tcacatgatc gtgcggaggt acggaagacg
     5641 gggttgaggt cagcgaagaa ctgcacgccg caagcagaat aaagagagcg acagaaaccc
     5701 ctagtttctt gaaactcgta gctttaaacc atttgtaagt cattcgtcga tcaatccctg
     5761 ttcagaaata tactctgcaa acaggcgtgc cttttctacg gcgccagctt cattgacatg
     5821 ccgaccatct gcccagtatc tcgcatcctt tggcatgacc gcagcaaaat cgaagagagg
     5881 gatttgctgc tcagtagcta cttcctttac cacatcattg ttttctgcaa acgcgagctg
     5941 ataatagtct tcggctgcat agtcattcaa aaatggggaa tgagcccagg tcgaaaggag
     6001 gattgtaata ttgtgggctt ttgcgaccgc agtcatattt tccaaatttc gtcggaaata
     6061 aataggcgga ttctccttta agatttcatt tggatcgaga ttatcctcaa taagacggta
     6121 ttcaaagggc cagctaacat aagtaggtga actcacaaaa tcatcaatgg aaacttgacg
     6181 ggtaaagctc agcattctgc tggtaatcct taatgcggcg ctatgttccc acaaagcgac
     6241 cgccgggact tgccatgcct gccgtcttcc taaatcgtca ccgcgatatg aggctggttc
     6301 caccatgcgc gcatggacat cattcgtgcc ttcataaata atgaccagat ccggatcaag
     6361 atccaatacc cgaaattcga ggttgactag gatttcccag gagttatagc ctggcactcc
     6421 cgcgttgatc acctggacat tctgatagcc ataatcgtcc ttgagcatct tctccagttg
     6481 ggcagtaaag gtgtcggcat tatccttgat actcacatca tacgtggatg aaccacccaa
     6541 ggctacgatc ctatatactc catttggttt ttctaaggga aattcctcac tccgataacc
     6601 cagggaatta tgtgatgtaa gtcccttttt ataattcgga gtagggtaat aactgagata
     6661 aggatggggc gcaaaggtaa attcctttgg atcaatactg gtaaatagta caaatcgatc
     6721 atattgctca ggcgtagcca ggtaattaag ccacactctg gacatcatct cgagcaagaa
     6781 aaatgtcagg aacgtgctga acaataccag gacaacgtca gtcctttttg gtagcttaac
     6841 ctctggttgc cttatcagga gaaaaaatcc agcggcgacc gccagaagtt gaaagacaaa
     6901 aatcaaagcg ttatagagcg gtagctctat ttgtccgtca gaaattagcg ttgactcaat
     6961 aagccgcttc agcaaaactc cgaatatgat cagaatgatg cccaaataga aggactgttt
     7021 acgatgtatt tttagtggat tcattgctta tgttcgaact gttctacttg tcagttggaa
     7081 gaatccaacg gtgtgacatg ctaacgctga acttcccatc tggtcgaaca ggacgtccgc
     7141 gtacgtgacc ttctgcaact tcgaacaccg tagcagcctc caggaaatcc tgtaggcgat
     7201 tgtcgccgat aatcgctaca tcgacacggt atcgccctgg cagcaatagc agttcatcca
     7261 gatcgcagat aaacttcatg ccgttctcag gatcactgga gtcttcgggt gctcgcacgc
     7321 ggctctggaa agtcgtcacc ggttgaccga tggtatcgta aacattgaaa ttgcatgcaa
     7381 tccccggtac aagcttatcg atctggaagg taaaacgcgc agggtgtcct gttctcaaaa
     7441 tagaggacga cccattggtt acttctgctc ctatgagatg cacttttccc tggcctttcc
     7501 tgtccgttcg ttcagaaagg tcaagcgctt ccgcctgctc cagagtctgg aggtaagcat
     7561 ccaccgcctc ggtaatggtt ccatctgtgt gtactgtgcc atgctgtaga aatatgccgc
     7621 gttcgcaaag ttgctgtaat aatcccatat tgtggctgac gaaaaccaca gtgcgccctt
     7681 cctgtgcaac gtcgtccatc ttgccaaggc actttctctg aaatgccata tcgcctacag
     7741 ccaatacctc atcgacaagc aagatctctg gttccaggtg agctgccacg gagaaggcca
     7801 agcgcacata catgccgctt gaataatgct ttaccggtgt atggagaaat ttgtcaattt
     7861 ctgcaaaggc tacgatctca tcaagtttgc gttcgatttc ggcttttttc atgccgagaa
     7921 tggcgccata cagtgaaata ttttcgcgcc cggtgagttc cgggtgaaag cctgtgccta
     7981 cctctaacag ggaaccaatt cgtccgtaga gatctgcata tccgcgtgtc ggttcggtga
     8041 ttccagaaag gattttgagg agtgtgcttt tccccgcgcc attgcggccg atcataccaa
     8101 ctacctcacc gggcttgatt tgaaaggaaa catctttgac tgcccagatg gtttcgtcaa
     8161 gttcggcggc tccgttagcc tggccgcgca ataacttacc agcgcggcga agtggtgtca
     8221 gagccatatc ggccagctgg tcggtcaacc ggtggtacgt cttctgtggg ccaccaatat
     8281 ggtactcttt gccgagaccg gcgacctcaa ttgcgatttt gttcattgga ctcatttaca
     8341 gtacatccgc aaaattctgt tccatgcgcc gaaagtaaaa agcgccacca acgaacaaga
     8401 ccaacgccac caccgccgaa acgagcgtca tgggacccgg cgcggtatct gtacccagca
     8461 gagcccagcg aaatccttca accacccctg ccattggatt taaaccatac aaggtccgcc
     8521 acggttcagg gagcaaactg cttgggtagg caattggtgt gatgaataac caggcctgtg
     8581 tcagaaaagg gatggtgtag tgaacatcgc gaaactggac gttcatggct gaaagccaca
     8641 gagagacacc cagagatgtg atcaatgcaa gcagcaagaa aaggggtagc catagtatgt
     8701 tgatcgtggg agttcgtcca taaaagaaca tcattcccat aagtacaagg aatgctaacc
     8761 caaagtcaac gacgccagcc aaaaccgtcg caataggcaa ggtcagccgc ggaaagtaaa
     8821 tcttcttcac catattggcg ctcatcacca gactatttga tgcctgagtg agcgcggtgg
     8881 caaagaaggt ccacgggacc aacgcagcgt agctgaaaat gggatagggc aagccgtctg
     8941 aaggaatact agcgaggcga ccaaagaaga ggctgaagat caccattgtg aaaaatggtt
     9001 ggatgatcgc ccaggctgcg ccaagcacgg tttgcttgta gcggactttg atgtcgcgcc
     9061 agacaaagaa gaaaagtaat tctcgatact cccacagctc gccaaatttt ggtaacgccc
     9121 atccgctgga tggttgacgt cgagttcgtg gcaggtcgga tgatgtaagt gtagtttcgt
     9181 tcatacagaa ttgctcttag cctggtgcaa gctccacctc taaaatcttg tctataagtt
     9241 cgctcagatc ggatgccttc atatctggct ggggcccgaa tggattcata acctgcccgg
     9301 gcctagcgac gaatgcggca gcacagccag cctggagagc gcccgttaca tcccaatgat
     9361 gagccgaaac caaccgcatc tcttttggct caacacccag ccggctggca gcatgatgat
     9421 agatggatgg cgctggtttg aaggattgga cctcgtcggc ataaatcacc tgttcaaagt
     9481 ggttgcttaa accggcaaac tcaagctgag cttctgctgc ggcgaccgtc gagttagtca
     9541 gcgcagcgag cctaagaccc gcatcacgca atcgctggag actcggagcg acatcgggat
     9601 acggacgcaa cttgcgcaat gttcccagga taaggttctt ttcttctcct aacatcgaga
     9661 ctttgtagtg ccgagccgtt atctccagag ccgtgtcagc ctgcactccg aaatcctcat
     9721 aagtattcgt caacatggta gccagcgcca gttggatggt ttgcatgaac cactctttcc
     9781 gtaccgatgc atctccgaag aaacgttcga agtgctgatc catggtgcga agatccagca
     9841 gggttccgac caggtcgaaa acaatcacac gcgacatgtg ttctccttaa tttgatatct
     9901 cttgatcgaa aggctttcta agcctcaacc ccttgctgag tgttctgttc cttgtcaacc
     9961 tgtttcagga tgtcatcaat gggcacgtca gtgagctgtc cacggaatac atgcctttca
    10021 ttgttctcaa cagcctcgat gtaacgtcgc ataacgttca atactttcac atcctcgcgg
    10081 ttgaagacct cgccctgcag caggcgcaat acctcgacgc gatattcact ctgaataaac
    10141 agcgtgaaga gtggtagcaa tttgtagtac aggcgaataa attcatacca gatatcgacc
    10201 ccaccgcgca gggtttcctc atagggtttg aaggaggctt cgctcaggtc gcctgattct
    10261 aacgcggcct ggatcgtctg ggcggcgaat cgggcgctgt gcatcgcaac actcacgccg
    10321 gatgaaaaaa tggggtcgac aaaacgagct gcatcaccta ccatcaggaa gccatttccg
    10381 cagaacttat cgaggatgta actgtaatca ccttcggttt tgaattcatt gacacgcaca
    10441 gcgtttgcca tcgccttggc taagccctta ttggactcaa cgtaggtgtt gaagtaacgc
    10501 tctgactcgg atttaaactg ttgaaagacc tcgcgctctg caactacacc aacggaagtg
    10561 atcttatcgt tgatgggaat ctgccacgcc cagccgcgct tgaccggtag gaagtaaata
    10621 tggatataat cttcggtttt cggatcctca ccgcgggcga cgttttcgaa ccaaccatgg
    10681 actgcatact gattgaaaat tttatcgttc tgtttcaaat taagttggcg tcccaacagc
    10741 gtttgccggc cgctggcgtc aaccacgatt ttagcgggga tgtcgatctc ctgtcctgcg
    10801 aatctggcgc ggacaccgac agcctggtcg ccttcaaaat tgacccgcct cacctgcacg
    10861 ccctgatgaa cctcagagcc caggctttca gcgtgcttaa gaagcaaaag gtcaaattta
    10921 cttcggtcaa catgatatgt atggtctcga tcaatgccct cctgacgaaa ttcagcgaag
    10981 tgcaaggcct gttcttttcc gttgaaatcg cgccacgagg cgccgaactt ctttggaaac
    11041 ccggcttgat ccattttgtc gatcagacca agttcgccga agaccctcat cgtggacagt
    11101 accagcgact cacccacgtg aggacgtgga tggttgtgtc cttccagaat caggtttttg
    11161 atcccggcct ttgacaaata ggatcccagg gttgagccag ctggacctcc accgataata
    11221 atgacatcgt acatgaatga tctccttgca gtgaaatgtt agttgctgtg gtttatagta
    11281 ctacgtgact tgaaagttta tgtgacaaaa ctttacagcc gctctgctac agggttcacg
    11341 cctgtcaacg cgttttttga gttaaactgt tggatagcgg ataatgcggg actggtcata
    11401 aaagtcgtca ccagtgccat taggaccatc attgtaaaaa cggtcgggga gagcacaccc
    11461 aagtcgtagc caatgctgag aataatgagt tccatcaagc cgcgagcgtt catcaacgtt
    11521 cccaatgcaa gagactcaga ccatgattga cccgtgaatc tggctgccac tagactcccg
    11581 ccaacctttc ctgccaccgc cactaaaatg attagtccgc aaatcaacca agccgagccg
    11641 tcattcaaga ggccaatttg agtctttaga ccagtggaag cgaaaaacag aggtaataga
    11701 atcaccagac tgacgtcttc aattttggct gccagcatct ttctgaagcc aaccgaaact
    11761 ggcatgacaa ctccggctag aaatgcgcca aataatgcgt ggatacctat cacctctgtc
    11821 gcaaaagagg acaaaaacag tacgacaaat gcaactatgg tgaaagaatg gtttgggttt
    11881 tcgccgctac cgtagcggac tgataggtgt ttgaggatgg gtctcaccag tacaagcatg
    11941 gcgaaaacgt aaaggacgga taggagaatc gtgaacaagg cgctcgtgac aactccagcc
    12001 tgagcgatgg cgatgacaac tgctaagatg caccaggcgg tgacgtcatt ggctgcagca
    12061 catgtaatag ccaaaacgcc cagcggtgtt tgagtcaatc cgcgctcttg aatgatccgg
    12121 gccagcaccg gaagcgccgt gatgctcatc gaaattccca tgaataggcc aaagctcaaa
    12181 aaggaaattt gcgggggagc caatgtcttg tataaaaaat acgcgagact aaggcctcca
    12241 aaaaaaggga aaatgatact ggaatagctg accagaagag ctgtatgtgc tttgtgtttg
    12301 agcacatgaa catctaattc cataccaata ataaacatga acagaatcag tccgagttgg
    12361 ctcagaatct caatgttggg cagggaatcg ggcggaaaca gaaaattcgt cacttccggc
    12421 cagaacagtc caagcagaga agggccaagc acgatacctg ccacaatttc gccaatgact
    12481 cggggctgac caatttttgc caccagaaac gccaacgatc ttgaagctac gacaatcaca
    12541 gcgatttgca gcaacaagcg gcttagcgga tgtgctgtgt tttgggcaaa acctcgaacg
    12601 acgacacgca gtgcgctctc tggcgcggta acgctatttt gctcactcct aaccatgtca
    12661 atggtctggg gattctccaa ccgcacaccc tggttgagaa caaaccagat tagaagaccg
    12721 aaaatcgcga tcgtcagtgt ataaaagacg agatttcgat tcatactaga gccctagcca
    12781 gcgcgcgatg atcatacgtt gaatgtcgga ggtgccagag taaagagtgc cacccacagc
    12841 atcccgcagg tcgcgctcaa cttccatttc agtcatataa ccgtaaccgc cgtacgttcg
    12901 aatggcatcc atacttgaat cgacgaaaca ctcgctcaaa tatagttttg ctagtgccgc
    12961 ctccatgatg gctggtttgc cgttctgatt gagccatgcc actttgtaca aaagtagtcg
    13021 agcagtttcc agcctgactt tcatgtcggc aatacgattt gcaacagact gaaactttcc
    13081 tatgggctga ccaaactggc ggcgctcacg ggcgtagcga atacccgcct ccagttgtcg
    13141 ttccattgct ccaatgtgac tacccagaat gcaactgcgt tcccattcca tcgagctatt
    13201 aaaaatgctg gagccagcgc cctccgctcc taacctattc tcaaccggca agatacagtc
    13261 ttctaaaatc agctcaccca tgggcgaagt tcgtagaccc atcttttcga tattcttgct
    13321 aactctgaag ccaggagttc ccttatcaat cagaaaggcg gtgacacctc cgataccctt
    13381 ctctggatta actgtcgcaa acaccaatgc catatcggca accggtgcgt tggttacgaa
    13441 catttttgtc ccgttcagga tatagccgtc gcttgtccgt tctgctctgg cgcgaagtcg
    13501 gtacgcatcg gagcctgagt ccggttcaga catgccgtga gccccaatca attcaccact
    13561 acacatacct ggaagatact tctgcttctg ggcctccgtg ccaaaggtga gaatagggtg
    13621 ctggacactc cacatttggg cattcatcgc aaaaattaac ccgttgtccc ggcagccata
    13681 ccccaatccc tccataacca gcatggtggt catgatgtcg gtcgcgctgc cgccgtaggc
    13741 ctccgggata cctaatccca ggatgccaag tttggcgcac tttatccagt tctcgcgtgc
    13801 cagctcaccc tgtctgtctc gatcaatcaa cccctcgttt aattcttttt tagcgaactc
    13861 gatcacagta ttcttgaaat ccagttgctc ttttgtccag gaaaagtcca tcagattatt
    13921 gtaaatctcc ttttcggcga tgtcgcaatg cttcagtgtt tgatgattcc aggtaaaaac
    13981 tcagcaagtt cgttcgcagt caatttgttt ccctcagccg aaggatgcat ccagacatcg
    14041 gcaaataaat atctgtcttc aagttgacac tctccgccgg gcttttgcag acaggcttgc
    14101 cgaaaaacag gcaaaagatc caatactggg attccagctt cttctgcctt cgcagcaaac
    14161 aactcctgcg gaagtgtcga ataactctca tcgattacct ggaattccaa ggggaacatt
    14221 accagtacga tcggaatatt gttttccttt gcgaggcgat tgatttccaa tatggtgtcc
    14281 cagcgctcgg tccatcgttc agactgagga tctaatggaa aatactttgc cgcgttggtg
    14341 ggaggatcaa ttgtgtctac acggtctcgg ccctcagcac gcgccttcgt ccatgacaat
    14401 tggttctgga ggaaaggcca aaaataggtg tgttcattta gccaccgcaa gggctgtggg
    14461 tcattgcctg ccagtagggc gctcttacca ttgatatcgt tggcgatcgt taaatcgagc
    14521 actactacat ctgactcata cttcattccc tctgcctgga aaaatgccag ggcgttgtcc
    14581 aaactccaac cgggtactcc tgcattgatt acttcaaaat ttgatttgct gatgccatct
    14641 tcattgagca acgcttccaa ctgttgccca tacgtatttt cctcgctaac tccccagccc
    14701 atcactactg aatcgccaag gtttaaaatt cggatcgtct cagaaggctt ttgatacgtt
    14761 gtttcgggtc cgcgtaaccc atgcgaatta atgtccacgg agataccctg ccactcataa
    14821 tgactgttgg gctgcataag cgtatatccg gcgcccggtt cgggatgttg gacccagacc
    14881 atccgacgcg atgcgactaa ttcccaggca taaagactat tggcctgctt tcgctcccag
    14941 aagtaagccg cgccttcaag cattgcgatc agcaaaagtg ggctcacaac agcaaggata
    15001 acggtcagca atctttctgc ccgtttgctc agggctgggg ttttgaggtc tgaggatgtg
    15061 gttaaggaag acataacaac ctgagtaact aagcccgggc ttcgtcagag atcaggttcc
    15121 ggtggcgcca gttctttgcc catttgacca attcaagtac agcttgggcg gcaaatacca
    15181 ttgccactgc gtcgaccgtt aagcgatatc tgggtgcagg ccatgagagt aagtggatag
    15241 tggtatgaac caaaataaat aaatatagca ccacgaacct gcgccagcgc gagaaagaca
    15301 aatacaatcc aacgagcata aacggcagat atagaccaaa agaaagcacg cgatttatat
    15361 tgctgattcg actcgacgta gcagagggcc aaaaccaaaa atactcgaga gttttattca
    15421 aagttaagaa tacataccgt tcaggatccg aaataatata ctcaatccct tttcgagtca
    15481 gagcacgatc aagctgtgct tcattctgtc ctttcagctc ctggggcaca ggcactacca
    15541 ctaactcatt atcccaattg gttcccagac cgggattgtt ggaagcaaat agagcatacc
    15601 cagcgtttga gttcaaaagg agaaactggt cataaacccg ataatttctt atggtccagg
    15661 gcataacaaa caagatgatg atgatgatag gaatgctgaa gtgccaccaa cggctgctct
    15721 tgtcttttag ttctgcgaat aaccaaagga gtagtatcgg agcaaagatg agaacagttt
    15781 gtcttaggag tacggctacc ccaagcgaac agccgagccc gacccagcga atcaaggtag
    15841 gattttcctt gatctcaata ctcagataca gcgtaagcaa taccaaaacc ataaaaaatg
    15901 tttcagtcat aagggcggcg ttgtaataaa taaaataacc gtagacagct gccaacgctg
    15961 cagcggccag tccggttgct tcgtttacga ccctgcgacc gattttgtaa atgagaaaac
    16021 aagtcaacac gctgcccaca acggtctgaa gaatccgcgc tatcaacgga tgatatcctg
    16081 atatggcgta aattccagca agatagactg gatagataaa tgaccagtga gatgtgggcg
    16141 tattggccgg tgtgaacgga taccaggcct ctcgaaagct gtaacctcga ccatctaaaa
    16201 ggctgcgagc cagagcatcg taagaaatct ggtcctgtat gcccggcaat tctgtaacct
    16261 gatctcccat ataagcggca actggtattc gaattagtgc ggccacaatc aagatgagag
    16321 tcagtacctg cctgtttcta tcactcttga gcatgacaga cctcaagcgc aacatcataa
    16381 acgcgttggg ctaacttatc aatcgtgtga ttttcttcaa agcgcagcct ggcttctctg
    16441 cccatccgtg cggcctcatc cggattttgc cataattgta ggattgcctc tcgcataccc
    16501 tgaatgtcgc caggctccac aagcagccca gtctgcccgt gctcaacata ggtttctgtg
    16561 ctgggtgcgc gtgttgcgac aacagccctg ccaaagctca tggcttggag cacactgctg
    16621 tcgcctccag cgaaggtgat aggctggaga gggaccacgt aaaaactgga tgagtataag
    16681 taatcctgaa actctcgctc agccagccaa cctgtagctt ccatgtttat tgggaggtca
    16741 attcctgcca gattgaaggc gcgaccgctg accttaacat ttacatcagt tccctcgacc
    16801 gcgctggcta aagttccata atctctgtat gaacgcccag aagcgagaat aaattttcct
    16861 gccctcgccg cggattcaga cttattcaca ctttcaggat cataccaact catcggatca
    16921 taccagccat gaggagcgta actaatctta ttagggggca aagcgaggag ttgctgatat
    16981 tgttccactt cacctggagt aaataccacc actcgcgaga cggaacgcaa tccaaaccgc
    17041 gccagactgc gaaagtggct gataacacca cgaatattga atgccaaaat gatcagtggt
    17101 gggtgattct gaccgacgag actgcgtatg agtgcgtacg gaaaaccatt tttaccttcc
    17161 caagctacca caagatcata gtgtgtctga cgagtcttcc gtaaagcctg gaatcctgcc
    17221 tggaaatagg ttggatagta attgagcaac ttaccccact tttgaaagcg gtccacggcc
    17281 cccgtggcaa ccaggaaatc tatctcatca ttatttgatg gtaagtattt ccatagccag
    17341 cgatcaccct gctttacttc attgtcaagt aagcacaaca ccttcatcta tgatgtccta
    17401 gtttcttaat ggattttctc gatatacttg ttaattagac tggccgctag atcgtgccca
    17461 tgttgattcc aatgagtgtc aaatcgatag tagagttctg cacctgcggc cgcttcttct
    17521 tgaaaggttg aggtcaggtc gagataaagt atcatgtggt ctgcagcaaa gtcagccagc
    17581 agtcctgcct gatcatccat atgtcgacga gttggttctg gttttactcg ttcattcgtg
    17641 aactgtagaa atcctgcttc atccagttct actgcgggca ctcccgtaaa gacacgctct
    17701 agaatatttt catcatttaa gtatggcaga tagacatgtt ctttggatgg tacataaact
    17761 aatagaaagc gagctccagc tgcctcgctc aattctaata cttgtaaaat tgtttcgctc
    17821 accaaccgaa aattctgcga tgattcgatt actgcgcgat caaccgataa ccaggaaaaa
    17881 tagtatgaaa aaaaggctac ctcaaggtct ttattattaa ttgcgatatt aataggatat
    17941 tgataattcg agcgaacctt gacaggtgct acaccccaac tcttctcatt ccaagatttt
    18001 atactttgat ttagcgcgta ctttccaaat cggaaaagga taaacggatt tgcttgttca
    18061 taagcagcag aatcgtataa atcattgccc tcaaaataag ccataattac ccactgaggt
    18121 tgctttttta taccatattg ttgcaaaacc tttagttcgt cctgtggtcc aaaacccaca
    18181 tctccaagat tgagcacatc tcttttagca tactcagcca gtttttgcgg ccaggaatag
    18241 gcaacgccac ttgctctagt aaacgagtcg cctaaggcaa cgatgtcata agtagcttgc
    18301 tcctctggag aattgcggaa accgttagcg tcggtgacca tttgtacttg agctactact
    18361 tcatcttcat caggcgacaa tggcgcaatt tgcccacgca tataatagaa taggtcgccg
    18421 tcagattgtc ttaaataata gctttcgtcg ataaatgcct tcacgcgacg agcgggtgga
    18481 ttaactctta catcaggtgg tatccaattc gggaatgccc tcattagcat ttctaccatc
    18541 gccagcgaga tcaccactcc caataagact agaaaaacat ttccaagaac aggtacaaag
    18601 gctctcttta ttacttgttg ttcattcatc tactggcgct tgattgaaaa agagggattt
    18661 cctcttcttt ttcacgaagc ttacgataga gatcctcagc aatcagcatg tgtcctttgg
    18721 cattcgggtg cttatcccaa taagcaacca caagggaatc cagatcttgg ttatcgtacg
    18781 catcggaaag gtcaaggacg atgaaaccag actcctcggc tagcccggtg aggtagataa
    18841 tttcctcctc gtgtaacgga tcttccaagg ttggcatgaa gatccaaaca ggcaaaatcc
    18901 cgtgggaatg agaaacttcc acaatctgtc tatatgtcca caagaggatc tcggatccgt
    18961 aaggtttgag aagtcgttca gcctctgcct tcgtcattcc cgcctcgact ccggcccggt
    19021 gggcgagttc gatgagatcc gcgtaaggaa actcaactcc agttgttaat cgatctgcga
    19081 ggtacaaaac catcgcatcc tcttcgcgtt gatgagccat aacaaagatc gcgttcggtt
    19141 gaaattgcag agccttttgt tccaggacca acaaattttg gaatgcagaa taaccgggaa
    19201 cggcgaagtt cagaatttca tacttcgtat acgcgttacc agaattttcg tgatttaagc
    19261 gatcctccaa cagtggttcg aaaccttcat catctgcaac gccatacccc atcacgaacg
    19321 agggtccagt cagggcaata cgatatgtat ctggggcggg aatttgctca taatcgcggt
    19381 cgcgcatgcc ccatcgattt atgcttaatt cggtaccgtt gaaatcgatc tttgttgatg
    19441 gagcaagctc taaaataagg tcgtcgttgg tcagttgaat agctgcggta tctcgaagcg
    19501 cgacccagtc ggatggcctc ttcgtataaa tttcccaaag ctcagaatta aaccgattca
    19561 cgccgatcag atcttcgtag taaccgcgct ggagaagcgc agcttcccgg tcgctgagcc
    19621 tgtctacagt caggtcactg attaattctt gaggtctgcc tccaaactgg ctatagacaa
    19681 caggatttcc aattaggaat aacagtacaa tgccgctggc agttgcggcg gtagtccggc
    19741 ggaaattatg ttgttttgac tttcgttcag tattatcatt gctacgcgag ctaatccaga
    19801 gtgtcaggcc aaaaatcact gataacatca gaaaaaccaa gagcaatagg gcgatgtttt
    19861 cgggggtaaa ttccaccacc gaaatgaggg caatccaatc ctggatagaa tcgctgctcc
    19921 ataaggacca aagcactgcc atcacagtga atgtgcctgc tgtgcggagg gccaatgaag
    19981 tgagatcccc aactgacatg gaacttcgtc ctaaggcccg tttacggccg cgccggttct
    20041 catacagtgt attgactatc accaaacatg ccaaaataaa ccagaacacc atatctggtg
    20101 cggtaaaaag gaatgttcct cgaagccaga accactggta tgcatgaaaa aaccaggtta
    20161 gaaaaaatac aaaggcggtc gcaaagacaa gactactatt tggtccccat tgccggattt
    20221 tgaagtaggt gggatagtaa aaaaccttca acatgaaatc tttccagtag atattgatac
    20281 gccgccataa atcagtaaaa ctggatgcca tgaaatagag gtggtgcgtt tccggcaagt
    20341 tgaagccaaa caaatgcagg ataccgatga tcacgtggaa ctgtccggaa attcggagat
    20401 aaagggcaaa atttgaaatg agataacgaa ccaactcgag agtattggtt acatcccctg
    20461 gggcaatcat aatgtaatag ttgatatatc gatacagaat gagctggatc gcacctcgga
    20521 agatccactg caggccccgt tgataaatca ggtgctgttc ctc
//
LOCUS       DASTJP010000159         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_780974, whole genome shotgun sequence.
ACCESSION   DASTJP010000159 DASTJP010000000
VERSION     DASTJP010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780974"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..677
                     /locus_tag="VFQ23_04890"
     CDS             219..677
                     /locus_tag="VFQ23_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET9905948.1"
                     /translation="MEHPTIFSITEDELDLNDLLARITLTSTGAAAIFTGMVRGLTTR
                     DASHETEYLEYEAYIPMAEAKMKQVAEEIRERWPVIEGIAVVQRIGKLYPKTPTVLIA
                     CTAAHRDTGVFEAARYGIDRLKEIVPIWKKEVSPDGEFWVEGDYIPKSGE"
     gene            754..1989
                     /gene="fabF"
                     /locus_tag="VFQ23_04895"
     CDS             754..1989
                     /gene="fabF"
                     /locus_tag="VFQ23_04895"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9905949.1"
                     /translation="MERVVITGMGTLNPLGLTVEESWKNAINGVSGVGPITLFDSTPL
                     NVHFAAEVKNFEPAQYMDPKEARRRDRYEQLGVAAAKDALASSGLEVTEANAGRIGVL
                     VASAVGGITSLQDAVITNHTEGPRRVSPFLIPMLMPNGAAGMIAIDNQIKGPCFSVAS
                     ACASGSDGIGTAFLMLKAGMVDAALAGAAEVTICSTGVAAFDRVGAMSRRNDDYSMAP
                     QPFDKNRDGLVMGEGAAVLVLERESYAKARGANILAELAGYGATADAFHVTAPHEHGA
                     GGSAAIRMALDSAQANVDELGYINAHGTGTPLNDQSETRAVKAALGEKAYEIPISSTK
                     SMTGHMMGATGALEVIFCVQAVREGILPPTIHYETPDPECDLDYIPNKAREKKINLAL
                     TNAFGFGGHNAVLAIRKYE"
     gene            2128..2475
                     /gene="rplS"
                     /locus_tag="VFQ23_04900"
     CDS             2128..2475
                     /gene="rplS"
                     /locus_tag="VFQ23_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET9905950.1"
                     /translation="MSDVIKALEAKQNEKIPQLAPGDTVSVHVKIKEGERERIQEFKG
                     IVIRLRKGGTESSITVRRMASNGIGVERTFLLRSPRLDKVVVERHSQVRRAQLYFMRG
                     RTGKSARLKQKFD"
     gene            2550..>2755
                     /locus_tag="VFQ23_04905"
     CDS             2550..>2755
                     /locus_tag="VFQ23_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET9905951.1"
                     /translation="MKPLIGITTHLQSNEFGQTRIVLQQAYANAILQAGGVPVLIPSV
                     LAEDGWDVLYSRLDGVLFSGGGDIG"
BASE COUNT          702 a          643 c          772 g          638 t
ORIGIN      
        1 tccgcctgat ctgacagtgc agggcttgaa agacaaaatc tccggtgaat atccaagcct
       61 caaagagtca atgaagagtg tgttgatcac gatcaatcgc gaatatgcct tcgacgaagc
      121 agtgatcccg cctgatgctg aactagcgat gtttcctccg gtgtcggggg gataatcaag
      181 aatgttgtct taagacgtgg agcatttaac tgaatacgat ggaacatcct acaattttct
      241 ccattactga agatgaatta gacttgaatg atctgcttgc cagaattacg ctgaccagca
      301 caggtgcggc agccattttc accgggatgg tgcgcggtct gacgacacgc gatgcatcac
      361 atgaaacaga gtatcttgaa tacgaagcat acatcccaat ggcagaagca aaaatgaaac
      421 aagttgcgga ggaaatccgt gagcgctggc cggtgatcga gggaatagca gtggttcaac
      481 ggatcggtaa actatatccg aagacgccca cagtcctcat cgcctgcaca gctgcccatc
      541 gcgatacagg cgtctttgag gccgcgcgct atggcatcga tcgactaaaa gaaattgttc
      601 cgatctggaa aaaggaagtc agcccggacg gagagttctg ggtagaaggc gattacattc
      661 caaagtcggg agaatagaaa attgcccgtg tcacgttatt gggaatcgag ggttttacca
      721 aatgaaaagt ctaagttaca gtgtgaggat tgaatggaaa gagtagtaat tacagggatg
      781 ggtaccctca accccctcgg gttgaccgtt gaggaaagtt ggaagaatgc gatcaatggt
      841 gtctccggcg ttggtccgat tacattattt gattctacgc ctttgaatgt gcattttgcg
      901 gcagaagtaa agaattttga accggcccaa tatatggacc ccaaggaagc gcgacgacgc
      961 gatcgctatg aacagctggg tgtcgccgcg gcaaaggatg cgctggctag ttcggggctc
     1021 gaagtcaccg aggcgaatgc gggacgaatt ggtgtgctgg tggcttctgc agtgggcggc
     1081 atcacttctt tgcaggatgc agtcatcaca aatcatacag agggtccgcg gcgtgtgagc
     1141 ccatttttga ttccgatgct gatgcccaat ggtgcggctg gaatgatcgc gattgataat
     1201 caaattaaag gaccttgttt ttcagtcgcg tctgcctgtg cctcgggttc agacggcatt
     1261 gggacggcgt tcctgatgct gaaagccggc atggtcgatg ccgcgcttgc aggtgcggcg
     1321 gaggtgacga tttgttccac gggtgtggct gcctttgacc gtgttggtgc gatgtcgcgg
     1381 cgcaacgatg actactcgat ggcgcctcag ccttttgaca agaatcgcga cggactggtg
     1441 atgggggaag gtgcagcggt gttagtgctt gaacgggaat cgtatgcgaa agcacgtggg
     1501 gcaaatatcc tggcggaact ggcagggtac ggcgcgacag cggacgcgtt ccatgtcact
     1561 gctccccatg agcatggcgc gggtggctct gctgctattc gcatggcgct cgattctgca
     1621 caggcaaacg tggatgagct tggttatata aatgctcacg gcaccggaac tccgttaaat
     1681 gatcagtccg agacaagggc ggtgaaggcg gcactcggcg aaaaagcgta tgagattccc
     1741 atttcttcca ccaaatccat gactgggcat atgatgggtg cgactggagc gctggaagtg
     1801 attttctgcg tacaggcggt gcgcgaggga attttgcctc cgactattca ctatgagact
     1861 cctgacccgg agtgtgacct tgactatatt cccaataagg cacgtgagaa gaaaatcaat
     1921 ctggcattga ccaacgcctt cggtttcggt ggccacaacg ctgtattggc gatacggaaa
     1981 tacgagtaat tcttgttgag ccgatagaaa atgacggcac gtataaatcg caacacgaag
     2041 tgagtgatcg caggcttgta caaccccaaa ttacatgcta taatccgccg tcgctttcaa
     2101 agaaatcata aacaaggaag aaatcccatg tcagacgtta ttaaggctct cgaagccaag
     2161 caaaatgaaa aaattccgca gcttgcccct ggcgatacgg tcagtgtgca tgtgaaaatc
     2221 aaagagggtg agcgcgagcg cattcaggag tttaaaggaa tcgtcattcg cctgcgaaaa
     2281 ggtggaaccg aatcgtccat taccgttcgc cgtatggcct ccaacgggat cggcgtcgaa
     2341 cgcactttcc tgctacgttc ccctcgcctc gataaggtcg tggtcgagcg ccacagccag
     2401 gtccgacgcg cacagcttta ctttatgcgc ggacgtacag gcaagtctgc ccgcctcaag
     2461 caaaagttcg actagttccg aacatcagac cctaaggttt caactttagg gtcttttttg
     2521 ttttacaata aagcaggaac tccacaaaca tgaaaccatt gatcggaata accacccatc
     2581 ttcaatctaa cgaatttgga cagacgcgga tcgtgctgca acaggcgtat gccaacgcaa
     2641 tcttacaggc cggaggtgtc cccgtattga taccatccgt acttgctgag gatggctggg
     2701 atgttcttta ttcccgtctg gatggggttc tcttctcagg aggtggagat attgg
//
LOCUS       DASTJP010000160         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_591193, whole genome shotgun sequence.
ACCESSION   DASTJP010000160 DASTJP010000000
VERSION     DASTJP010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591193"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..336
                     /locus_tag="VFQ23_04910"
     CDS             136..336
                     /locus_tag="VFQ23_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905952.1"
                     /translation="MKSQIWMGLLSVLAFLHSGCANISQAASLAPTTEPLAVDLESGR
                     CQSGIRANSVLTYDKNGLDFWS"
     gene            333..689
                     /locus_tag="VFQ23_04915"
     CDS             333..689
                     /locus_tag="VFQ23_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HET9905953.1"
                     /translation="MKPYSQDLRERAIAALEAGQTQAQVAAQFRIHKSTLEKWWYRWR
                     DTGSCAALPLTHGPERTLQAAESFIRAEVKKRPDATLTELCERVEEAKGLVASPSMMC
                     RELKILNLPRKKVAPR"
     gene            703..1284
                     /locus_tag="VFQ23_04920"
                     /pseudo
     CDS             703..1284
                     /locus_tag="VFQ23_04920"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1716..2633
                     /locus_tag="VFQ23_04925"
     CDS             1716..2633
                     /locus_tag="VFQ23_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9905954.1"
                     /translation="MNGRFRPLVQHWSLSYITARQIVQPLQRLESKAAALAWGDFETI
                     KEPVGGISEVQHLQKELTEMANKLEAAQEGLHDYIGAITSAQEEERTRLARELHDDTI
                     QTVIALKQRVQLAQKLVKDQQGRQSLKELEELAERTLENLRRLTRALRPIYLEDLGLV
                     TALEMLARESSQANHLSIDFQQSGKERRLSREVELALYRIAQEALNNVVRHASAKKHA
                     TVSIRFEKEVSLEVTDDGIGFVVPKSPTDFAPSGHFGLLGNRERADLIGAKLEAQSDA
                     GQGTRLSVRLTDPTTHSNRKKSRSRSRES"
     gene            3049..3840
                     /gene="lgt"
                     /locus_tag="VFQ23_04930"
     CDS             3049..3840
                     /gene="lgt"
                     /locus_tag="VFQ23_04930"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9905955.1"
                     /translation="MITISIDPIIFQIGHFALRWYGLIVLTAIQVGIWLIAREVERKG
                     LTKDDFYSAALWIIIAGIMGARLFHVLDHWHEYSINPIRALYIWEGGLAIWGGLIGGL
                     IAAAIVCWKNSWHLPKLLDAAAPGLVLAQAVGRIACVITGDAMGKPTNGPFGFAYTSP
                     GAMVPELGVYYTPMPVYEIVANLVIFLVLWRWRKRNWADGQLFLIYLTLYSIERFFLA
                     FTSSYRLIAFGLTQSQIIALVGLTISAVLLSLMSRRLNQPVVHAE"
     gene            3846..4151
                     /locus_tag="VFQ23_04935"
     CDS             3846..4151
                     /locus_tag="VFQ23_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HET9905956.1"
                     /translation="MEENCYVEPIYKTAVSAQQLQQADRATLAIWGMGCQNCVTRVRN
                     SLLATEGVYGVDVYLNMALAEVMYDGKRVLPKQLLEAVAQAGNDGRHEYRAQLLATE"
BASE COUNT         1107 a         1095 c         1154 g         1074 t
ORIGIN      
        1 cctatgattc cgaggtttta ctctagcttt atacctccct caaatgaaat cgttccaaag
       61 aactggggta tctggggacc cgagcagtcc gaaagtagtc gtgagaacat aactaatttg
      121 atttggaggt gatgtatgaa aagccaaatt tggatgggcc tactttcagt gcttgccttc
      181 ctgcatagtg gatgtgcgaa tatctcgcag gccgcttcac tcgctccaac aactgaaccg
      241 ctcgctgtcg accttgaaag cggcaggtgc cagagtggaa ttagagcgaa ttccgttttg
      301 acttacgata aaaatggatt agacttttgg tcatgaaacc gtattcccaa gacttacgtg
      361 aacgagcgat cgcagccttg gaagctggac aaacccaagc gcaggtggct gcgcaattcc
      421 gcatccataa atcgacgttg gaaaagtggt ggtaccgctg gcgagacaca ggcagttgtg
      481 cggctttacc actcacccat ggtcccgaac ggaccttgca agctgccgag agctttattc
      541 gagccgaagt caaaaagcga ccggatgcta cgctcaccga gttatgcgag cgggtcgagg
      601 aagccaaagg cttggtggca agtccgagca tgatgtgccg ggaattgaag atattgaact
      661 tgccgcgcaa aaaagtcgct ccacgatagc caacgcgaga cgcggcgggt aaaagccctg
      721 cgcgaggact tccaaatacg attcgtggaa agcttgggca agttggtgaa acgcctgaaa
      781 tttctcgatg aaagtggagc aaatttgggt ttgacgcgcc tgtgcggtcg cgccgccccc
      841 ggtcagcggg ttgtggaagc cacgcccggc tattcgggcc ctcattacac cctcctcgcc
      901 accctgggct ggcgggaagt caccgcccct tggatctttg aaggttccat cgatcgcgtc
      961 gcttttgaag cctatgtgcg ctcgcaattg ctcccgacct tgcaccgggg ggatatcgtg
     1021 gtcatggata acctctcggc ccataccggc gagaccatcc gtcaactgat tgaagcccgg
     1081 ggagcacgct tggaattcct gccgccttat tcgccagact tcaacccgat tgaattgtgc
     1141 tggtcgaaag tcaaggcggc tttgcgcacc gccaagcctc gcacgctgga cgccttgttg
     1201 gagtccgtag cggaggcctt gcggtcgatc tcgttcactg agattcaaaa ctggttcgct
     1261 cattgtggtt atactttatc gtaaatcact caggaaattg ctctaattgg ggcattcagt
     1321 ggcctgtact cggtgtattg cgccaatttt ttacatttcg agaaaactcc attaccacaa
     1381 aagtgaaagc aggtgagtat gctctcggct gcttaccgag tcgctggcat ccgcttattc
     1441 aggaagcaat tgatattagg caaggacaaa aaggttcatc gtatcgtttt cgattcgata
     1501 gaatgatgga aactgtccgg tttcttaatt gcgtgataaa cctgtgtaat ggttattttg
     1561 tctcatgaga aaaaccagtt ctcccttctg gcgccgattt agcgtacagc tttttgccgt
     1621 tactgttttg ccactcaccc tgcttctcct tttcatcgca tttggaagca tgaccatgca
     1681 tcagagggac aggcgtgccc tggtcgggaa gcgtgatgaa cgggcggttc aggccgctag
     1741 tgcagcactg gagtctgagt tacatcaccg cacggcaaat tgttcagcca ctgcagcgtc
     1801 ttgaatccaa ggcagcagca ctcgcatggg gtgattttga gacgatcaag gaaccagtgg
     1861 gaggtatctc agaagtccag catctccaaa aagagctgac ggaaatggcc aataagctag
     1921 aagcggcaca ggaaggctta cacgattaca ttggcgcgat tacatccgca caagaagaag
     1981 aaaggaccag actggcgcgc gagttacacg atgacaccat ccagacggta attgccctca
     2041 agcaacgtgt acaactggct cagaagttgg taaaagatca acagggacgt caatccctga
     2101 aagaactgga agaactggcg gaacgaacac tggaaaatct tcgacgactg acccgtgcgt
     2161 tacgcccgat ctatcttgaa gatctgggcc ttgtgactgc gctggaaatg ctcgcgcgtg
     2221 agagcagcca ggcaaatcat ctttccatag attttcagca gtcaggaaaa gaacgtcgcc
     2281 tctcgcgcga ggtggaattg gctttgtatc gcattgccca ggaagccctg aataacgttg
     2341 tacgtcatgc cagcgcgaaa aaacatgcga ctgtatctat tcgcttcgaa aaagaggtca
     2401 gcctggaagt gacagatgat ggcattgggt ttgtcgtccc gaagagtccc acagattttg
     2461 cgccgagtgg tcattttggt ttactgggga accgagagcg agccgatttg atcggtgcga
     2521 agctggaagc tcaaagcgat gcaggacagg gaacccggtt atctgttcgg ttgacagatc
     2581 ccacaaccca ttcaaacaga aagaaatcac gttcaagatc aagagagtca tgaatcccga
     2641 taacaacaat tccagcaaaa acccctctga tgaatcgaaa cagtcatctc tccccaaaac
     2701 cgcatgcggg ggtctgctgg atcatccaga agattttccc agcgcgatgt atcgtggaga
     2761 gcgagtttat ttttgcgcaa gggcctgctt gcaagcattc acccaaaatc ctgatccatt
     2821 tatggctgga gaaatcgaac atcctgtcga ttcgatctag cgatccgtaa gatcctcata
     2881 tcttcaagct ctggttcagg ccagagcttt ttttatgcag gcaaaaaggc ttacgcaata
     2941 acacgataaa cggcgcttaa gccctgcagg tcaaagtcat aaaatagctt tggtttcaaa
     3001 ccgaaggaca gtattgccag acactcttat gtatgaggtc caatatcaat gatcacaatt
     3061 tcaatagatc ctattatttt tcagattggc cactttgctc tgcggtggta cggattaatc
     3121 gtgctgacag caatacaggt cggcatttgg ctgattgcac gcgaagttga acgcaagggg
     3181 ttaacaaaag atgattttta cagtgcagcc ctgtggatca tcatcgctgg catcatgggc
     3241 gcacgattgt tccatgttct tgaccactgg catgagtact ccataaaccc aatccgcgcg
     3301 ctctacatct gggaaggcgg actcgcaata tggggtggac tcattggggg attaatcgcg
     3361 gctgcaatcg tatgctggaa aaatagctgg catcttccga agttactgga tgctgcagcg
     3421 ccaggattgg tcctcgcgca ggctgttggc cgcattgcat gtgtgatcac aggtgacgca
     3481 atggggaaac ctacgaacgg accctttggt tttgcctaca caagtcctgg cgcaatggtt
     3541 cccgagctag gtgtgtacta tacccctatg cctgtgtatg aaattgtcgc gaatctagtg
     3601 atctttctcg tcctctggcg gtggcgcaaa cgcaattggg ctgatggcca acttttccta
     3661 atttacttga ccctgtacag tatcgagcgg ttttttctgg cattcacaag ttcctatcgc
     3721 ctcatcgcct ttggcttgac gcaatcacag atcattgccc tggttggtct caccatcagt
     3781 gcggtcctgt tgtccctgat gtctcgtcga ttaaatcagc cagtcgttca cgcggagtaa
     3841 agaccatgga agaaaactgt tacgtcgaac ccatctataa gactgcagtc tcagcacagc
     3901 agctacagca agctgaccga gccacactgg ccatctgggg aatgggttgt caaaattgtg
     3961 tgacccgagt ccggaatagc ctcctggcaa cggagggagt atacggggtc gacgtctatc
     4021 taaacatggc actggctgag gtgatgtatg acggcaagcg ggtgttgcca aagcaactcc
     4081 ttgaagctgt cgctcaagct ggcaatgatg ggcggcacga gtaccgcgct cagcttctcg
     4141 caactgagta atgcgtcaaa tggctcacct ttctcttttt cagttgcggg ctgtccatcc
     4201 aggacagccc ttttagtaag cacatgcttt ctaaagcgta agcgaaaatg cctacgttcc
     4261 aacgggggat ttggcgctat ggctttcaaa ttcatcggtc tatactgggg acaacaggaa
     4321 ctacagaatg agcgcgttac ttcccttttt ggcacttaac caaaaccacg atcaggctga
     4381 agcatgggtc aaggaaaaat tgatcggtgc cggtttccgt gttgtgccaa
//
LOCUS       DASTJP010000161         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1397567, whole genome shotgun sequence.
ACCESSION   DASTJP010000161 DASTJP010000000
VERSION     DASTJP010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397567"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFQ23_04940"
     CDS             <1..1109
                     /locus_tag="VFQ23_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET9905957.1"
                     /translation="RFFEPTLKPHMDRASSSPTAPLSASVPDGMYLLADTVIAFDHAR
                     RSLSIIANVLDGDVESTNRKLDEIESRIHQPLPPVEKREVQVSKTRSNMTQGRFEDMV
                     RDAKEFIAAGDIFQVVLSQRFTRETNVEPFDVYRAVRRLNPSPYMFFFDFGIVDSEPL
                     FIVGSSPEMFVRLEGRTASIRPIAGTRPRGADAAADAALAEELLADPKERAEHVMLVD
                     LSRNDFGRVCEYGTVRVSDFFTIEKYSHVMHIVSHVEGKLRPEFTAFDLVRAAFPHGT
                     VSGAPKVRALEIISDLEPDSRGAYAGAVGYFGFDGNMDTCLALRTLIVRGNTFNVQAG
                     AGIVADSDPSTEFQETVNKASAMLKAIEMAEQNQ"
     gene            1124..1831
                     /locus_tag="VFQ23_04945"
     CDS             1124..1831
                     /locus_tag="VFQ23_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9905958.1"
                     /translation="MSKSSIEEIRTRFDNDVERFSNLETGQTATMDAPLSLELITQVA
                     AATNPDATHLLDIGCGAGNYSLKMLSKLPNLNITLVDLSQPMLQRAVQRIQAETKGEI
                     TTHQSDIRALSLPEAHFDIILAAAVFHHLRDDDDWQNVFAKCFAALKPGGSIWISDLI
                     DHEMPPARTVMWRRYGEYLTQFRDEAYRDQVFAYIEKEDTPRSLLFQIDMLRKVGFRQ
                     VEILHKNGSFAAFGAVK"
     gene            1848..2897
                     /gene="trpS"
                     /locus_tag="VFQ23_04950"
     CDS             1848..2897
                     /gene="trpS"
                     /locus_tag="VFQ23_04950"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET9905959.1"
                     /translation="MTNKPRLLTGDRPTGRLHLGHYVGSLANRVRLQHQYESFFIIAD
                     LHTLTTKPEPQYIKEIPTFVREMVLDYLAVGIDPDISTIFVQSAIPETYQLNLLFEML
                     VTVPRLERIPSLKDMARDANMDSMPFGLLGYPVLQAVDILLPRAKVVPVGRDNQSHVE
                     LAREIGRRFNNLYGEVFPEPDVIIGDVPLLVGTDGQGKMSKSLGNAIYLSDDVETVKQ
                     KVMNMYTDPKRLRATDPGTVEGNPVFTYHDAFNPSKAEVDDLKERYRVGKVGDVEVKQ
                     KLTVAINNFLEPFREKRAYYLAHPMIPNDVLANGIRRMQAEAKATMELVREKTGLSYS
                     LDLFVDQVDIFGTEG"
BASE COUNT          711 a          759 c          774 g          704 t
ORIGIN      
        1 ttcggttttt tgaacccacg ctaaagcctc atatggatcg cgcttcgtct tcgcctacag
       61 ctccgctcag cgcgagcgtg cccgatggaa tgtatctgct cgccgatact gtcattgcgt
      121 tcgatcatgc gcggcgcagt ctgtccatca ttgcgaacgt cctggatggc gatgtcgaat
      181 caacaaaccg caaactcgat gagatcgagt cgcgtatcca tcagccgttg ccgccagttg
      241 agaaacgcga agtgcaagtc tcgaagacgc gttcgaacat gactcaggga cgctttgaag
      301 acatggtgcg cgatgcgaag gagtttattg cggcgggcga tatttttcag gtggtgcttt
      361 cgcagcgctt cacgcgcgag acgaacgtcg agccgttcga tgtgtatcgc gcggtgagac
      421 gcctgaaccc gtcgccgtat atgttctttt ttgattttgg gatcgtggac agtgagccac
      481 tcttcattgt tggatcgtcg cctgagatgt tcgtccgttt ggagggacga accgcatcca
      541 tccgaccgat cgccgggacg cgcccccgag gagccgatgc cgccgctgat gctgcactcg
      601 cagaagaact gctcgctgac ccaaaggagc gtgccgagca tgtgatgttg gttgatttga
      661 gccgcaacga ttttggtcgc gtgtgtgaat atggaacggt gcgtgtttct gatttcttta
      721 cgatcgaaaa gtattcgcat gtgatgcaca tcgtttcgca tgtggaaggc aaactgcgac
      781 ctgaattcac ggcctttgat ttggtgcgtg cggcgttccc gcatgggacc gtcagcggtg
      841 cgccgaaagt gagagcgctt gagatcatct ctgatcttga acctgactca cgcggcgctt
      901 atgcaggtgc tgttggttat ttcggcttcg atggcaatat ggatacttgt ctcgcgctgc
      961 ggacgttgat cgtgcgtgga aacaccttca atgtgcaggc aggcgctggc attgtggcgg
     1021 attcagatcc atctactgaa tttcaagaga ccgtgaataa agcctccgcg atgttgaaag
     1081 cgatcgagat ggctgaacag aaccaatagt gcagaggctg aaaatgtcga aatcatccat
     1141 tgaagaaatc cgcacccgtt tcgataacga tgtcgaacga ttctcgaatc ttgaaaccgg
     1201 acaaaccgca acgatggacg cgccgctttc actggagctc atcacgcaag ttgccgcagc
     1261 caccaaccct gatgccactc acttgctgga tatcggttgt ggcgcaggca attactcgct
     1321 gaagatgctg agcaaattac cgaatctcaa catcaccctt gtggacttaa gccagcccat
     1381 gcttcaacgt gccgtccagc gaattcaggc agagacgaag ggtgagatta cgacgcatca
     1441 atcagatatt cgagcgttgt cgttacccga agcccatttc gacatcatcc ttgctgcggc
     1501 cgtttttcat cacctgcgtg atgatgatga ctggcagaat gttttcgcca agtgttttgc
     1561 agcgctcaaa cctggcggtt ccatatggat atccgatctg atcgaccatg aaatgcctcc
     1621 cgcgcggacg gtgatgtggc gacgctatgg agagtacctg acccagttcc gtgacgaagc
     1681 ctatcgcgac caggtctttg cttatattga aaaagaagac acgccgcgtt cactgctctt
     1741 tcaaatcgat atgctcagaa aagtcggatt tcgacaagtg gaaatcctgc ataagaacgg
     1801 ttcatttgcc gcattcggcg cggtcaaata aatcaaaagg agaaaccatg acaaacaagc
     1861 cccgcctatt aacgggtgac cgcccaacgg gacgtctgca cctgggtcac tatgttggtt
     1921 cattagcaaa tcgtgtgcgc ctccagcatc agtacgagtc ctttttcatc attgcggacc
     1981 tgcacacgct gacaaccaaa cccgagccgc aatacatcaa agagatcccc acctttgtcc
     2041 gtgaaatggt gctggactat ctggcggtcg gcatcgaccc ggacatcagc acgatctttg
     2101 tccagtctgc gattccagaa acgtatcagt tgaatctatt gtttgaaatg cttgtcactg
     2161 tgccacgact ggagcgaatt ccctccctca aggacatggc acgcgatgcc aatatggact
     2221 cgatgccttt tggcttactt ggttaccctg tacttcaggc agttgacatt ttgttgccgc
     2281 gcgcaaaagt agttcccgta gggcgggaca atcaatcgca tgttgaactc gcccgcgaga
     2341 tcggacgccg gttcaataac ctgtacgggg aagtattccc tgaacctgat gtaattatcg
     2401 gtgacgttcc attgctggtt ggaacagatg ggcaaggcaa aatgagcaag tcgctcggga
     2461 atgccatata cctctcggac gacgtggaga cggtcaaaca aaaagtaatg aacatgtata
     2521 cggatcccaa acgcctgcgc gccaccgacc cagggacggt cgaaggcaac cccgttttca
     2581 cctatcatga tgccttcaat ccctccaagg cggaagtgga cgatctcaag gaacgttatc
     2641 gcgttggtaa agttggcgat gtggaagtca aacagaaatt aacggtcgcg atcaataact
     2701 tccttgaacc gttccgtgaa aagcgtgcct actatctggc gcatccaatg atccccaatg
     2761 atgtcctcgc caatggaatc cggcgtatgc aagccgaagc caaagcaacg atggaactcg
     2821 tccgcgagaa aaccggtctc tcttattcgc ttgatttgtt tgttgatcag gtggatatct
     2881 ttgggacgga aggatagcaa atgcagaatg atgaatgcgg aatataaaaa caaaattctt
     2941 catcccgc
//
LOCUS       DASTJP010000162         6702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_218418, whole genome shotgun sequence.
ACCESSION   DASTJP010000162 DASTJP010000000
VERSION     DASTJP010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218418"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..644
                     /locus_tag="VFQ23_04955"
     CDS             333..644
                     /locus_tag="VFQ23_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905960.1"
                     /translation="MNEKHIQQVLEIEKQAQEVQEKAQREAQELPRLAEQEAQALIAK
                     AKSEAQEEARKIVAAAQAEDKANEVLADVEQKNRDIEAKANGNLDKAVAYVLERVIGR
                     A"
     gene            648..1739
                     /locus_tag="VFQ23_04960"
     CDS             648..1739
                     /locus_tag="VFQ23_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET9905961.1"
                     /translation="MASGGVTGYAAISARVRAMYSDLLTPQDMMRLSDSPDFQTLFST
                     LKTTSYGPYLDSLKDKEINPKRVISQVRRKLADAFYSVVQMAPENSRPLVKQLYRYYE
                     TGNLKALLRALVTVPSWSTTEITLWDRVRDVLFPMGSAAVLPAQAMVESGSVATAVDL
                     LRGTPYDETLAFAMRRYSAEQNLFPLEVALDLGYWRRLWAETKKLSGQDREQATKIIG
                     AFLDMNNLMWAIRYRVYHKLSEEEIINYTLPFGFRVQDTDIRAIAAGADIASIITRIY
                     PDITDASSLLENPQSGLPKLEYQFKRQLMKQCLAAFVGNPFHIGIPLAFLILSDLEVQ
                     DLIVLIEAKSSGIPDDEYRPFLLKTNPPS"
     gene            1812..3818
                     /locus_tag="VFQ23_04965"
     CDS             1812..3818
                     /locus_tag="VFQ23_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HET9905962.1"
                     /translation="MFFPREMTEVELIIPSKDLVAVAKVLSGHGVFHQVDSTYLGLEN
                     LGPSAWQERAASYSALERRIQTAMQTLNLAEDYTGSHDFDNIVDLDKVRPAVERIENE
                     VKNTSDQLSNERKRLEQLESQLHQLEPIADVNVEVSALRKSNFLHSTLGIIPAANVSR
                     LQTSLSRVPHAFFTLREDSQKPVVWLLGPRSNSDIIERAAKSAYLNPLSLPEEFDGTP
                     AQITSSIRKAIEASKQKISELEASLSKLSNTHRKELNELLWDVHVSRAMADAIVRFGQ
                     LRHTYVVVGWVPSADLKLLIERLKQASKEILIEAIPTERSGHHSNVPVALSTNPWLRP
                     IQNLVTTYGRPAYGELDPTLLVALSFPVLYGAMFGDLGQGLVLMLVGFLAHKRIFMKG
                     LQSLGLLLVYCGFFATIFGALYGSIFGFEGHLIEEYLHFHFEPLWMSPLENILTLLGF
                     AIDAGIVILIFSYLLGLFNQYRAGDWSHFWFGHTGVVALIFYISFLALLGGFLGTTAI
                     APRIAVAINNLPIPWTLLAGVFGLGVMFSGLLRNWAEGHRPLIEGKGVGGFIMFLVQS
                     FMDLFESVISMLSNTLSFVRVGAFAVAHGGLSLAFFSLAGEEPDLGFWITLLIGNIFI
                     IGFEGLIVYIQTMRLHYYEILGKFFHGGGMRFEPLTLTPRKEDA"
     gene            3903..4334
                     /locus_tag="VFQ23_04970"
     CDS             3903..4334
                     /locus_tag="VFQ23_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HET9905963.1"
                     /translation="MFLVFAVLVGLIPVIPAVLYVIQNGKTSPAQAVSKMVTGFNTFN
                     FLVGMMAAGLGVVWLASPSGAMASGLAQEGVVDQYATLAAALSTGLACIGAGIAVGGS
                     GAAAVGATAEKPESFGRALIFVGLSEGIAIYGLIISFLVLP"
     gene            4350..4655
                     /locus_tag="VFQ23_04975"
     CDS             4350..4655
                     /locus_tag="VFQ23_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET9905964.1"
                     /translation="MKVLVIGHPEAVLGFSLAGVSGRAASTAAEVNQALDDVQASKDV
                     GIVLVTQDVAELIPARMEHLKLRSTIPLVVEIPSPSGVPEGQASLGEIVLRAIGIKL"
     gene            4746..5366
                     /locus_tag="VFQ23_04980"
     CDS             4746..5366
                     /locus_tag="VFQ23_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HET9905965.1"
                     /translation="MKTEAQDIEMLARAILTEAREEADQLKADAQEKADGIRKRAQEQ
                     ADAERKAILDRAQEEAERLRGQSVATAQLKARSTQLTHREQLLDRVFKAVKEKLPEVQ
                     NRADYDEIAAMLLREALTQLRVHKANVRADEATLKSLKKGTLRDISKELNGEFKDGDA
                     LEEGTGVVVDAADGKLHYDNRLETRLSRLQGTLRSAVYQVLTGDKA"
     gene            5363..>6702
                     /locus_tag="VFQ23_04985"
     CDS             5363..>6702
                     /locus_tag="VFQ23_04985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET9905966.1"
                     /translation="MSDQVGTITWISGPVVRARGSRHVGMLELVEVGAERLVGEVIGL
                     KGDQMTVQVYEETAGMQPGAPIFGTGLPLSVQLGPGLLQSIYDGVQRPLPLIEAAMGS
                     FIERGARFDPIDRSKKWKFTPKVNVGDEVKGGQVLGIVMETDTFEHRVMLHPDDSGEV
                     TWLAAEGEYTVEEPIARVKKGKEEKEVGMLQRWPVRRPRPFVNRRGVDVPLVTGQRIL
                     DTFFPVAKGGAAGIPGPFGSGKTVTQHSIAKWADADIIVYIGCGERGNEMTEVLEEFP
                     KLEDPRTGAPLMERTVLIANTSNMPVAAREASIYTGITIAEYYRDMGYDVLLLADSTS
                     RWGEALREISGRLEEMPGEEGYPAYLAARLAEFYERSGRVICLGNHGNSDAQGRKGSV
                     SVVGAVSPPGGDFSEPVTQNSMRVASTFWALDYDLSRRRHFPAINWTSSYSLYN"
BASE COUNT         1604 a         1584 c         1849 g         1665 t
ORIGIN      
        1 tcactccaaa attcagtata ccaattccaa gtatagtggg aaggtgtaaa ggaaaagcgg
       61 cggaattgtg aagaatatgt gtggattctt agctgggcgg gctttcgtag cacagacagt
      121 attaatcgat ccgaatcggc atggagatcc cgacaatacc tatttccata tttcaattga
      181 catttgtgag tttcttcatt ataattgtgc ataatcacac aatcgaattc gagaaatctg
      241 atttgcggcg gcagaccaga ttttttgtat caagccttga tgatgaactc tttttacttt
      301 acaattacgc attccagtga ggtgaaatca aaatgaatga gaaacatatc cagcaggtgc
      361 tcgagatcga aaaacaggct caagaggttc aggaaaaagc tcaacgtgaa gcacaggaac
      421 tgccgagatt agccgaacag gaagcccagg ccctcattgc caaagccaaa tcagaagccc
      481 aggaagaggc acgcaagatc gtggcggctg cccaggcaga ggataaggca aacgaagtgc
      541 tggctgatgt ggaacaaaag aaccgtgaca tcgaagccaa agccaatggc aatctagata
      601 aggcagtggc ctacgtttta gaacgcgtta ttggcagggc ttaatttatg gccagtggtg
      661 gagtgacagg ctacgcagcg atcagtgctc gtgttcgagc tatgtattca gatttgctca
      721 cgccgcagga tatgatgcgg ttaagcgatt ccccagattt tcaaacttta tttagtaccc
      781 tcaaaacgac ttcctacgga ccttatctgg atagtctcaa agacaaagag atcaatccga
      841 agagggtgat cagccaggtc aggcgaaaat tagccgacgc gttctacagc gtggttcaaa
      901 tggcgccgga gaattcacga cctctcgtga agcaactata ccgttattac gaaaccggta
      961 acctgaaagc tttgttgcgg gcattggtga cggtgccatc ctggagcacg actgagataa
     1021 ccctctggga tcgagtacgt gatgtgctct ttccgatggg gtctgctgct gtgttacccg
     1081 cgcaggcgat ggtcgaatca ggcagcgttg cgaccgcagt cgatttgctg cgtggcacac
     1141 cgtatgatga aacccttgct ttcgcgatga gacggtattc tgcggagcaa aatctcttcc
     1201 cccttgaagt agcgctggat ttaggctact ggcgacgttt atgggcagaa acaaagaaac
     1261 tcagcggaca agatcgcgaa caggccacga aaattatcgg cgcgttcctg gatatgaaca
     1321 acctgatgtg ggcaatccga tatagggtct atcacaaact ttctgaagaa gagatcataa
     1381 actatacctt gccctttggt ttccgagtgc aagacacgga catccgtgcg attgccgccg
     1441 gggcagatat tgcatccatc atcacccgca tttacccaga tattacagac gctagttcgc
     1501 ttcttgaaaa cccacagtcc ggcctgccaa agctggagta tcaattcaaa cgccaactca
     1561 tgaagcaatg cctggcggca tttgttggaa atcctttcca tatcgggatc ccgctggctt
     1621 ttctcatttt gagtgatctt gaggtgcagg atttgatcgt gttgatcgag gcaaagtcct
     1681 ctggtattcc ggatgatgaa tatcgtccgt tcctattgaa gacaaacccg ccgagttaaa
     1741 tcctgatgtc tttgcgacgg atgcttgctc gcaggggccc gaagcaataa cacagaaaaa
     1801 ctggagatac tatgtttttt ccgagagaaa tgactgaagt tgagctgatt atcccctcca
     1861 aggatctggt agccgttgca aaagtgttga gcggccatgg agttttccac caggtggata
     1921 gcacctatct gggactggaa aatttgggac ccagcgcctg gcaggagaga gctgccagct
     1981 attctgcact ggaacgccgc attcagaccg ccatgcaaac cttgaatctg gctgaggact
     2041 ataccgggtc acatgatttt gacaacatcg ttgacctgga caaggtgcgt ccggctgtgg
     2101 agcggatcga aaatgaagtc aagaatacaa gcgaccaact aagcaatgaa agaaaacgcc
     2161 tggagcagtt ggaaagccag cttcatcaac tcgagcccat cgcggatgtc aatgtggaag
     2221 tcagtgcatt gcgcaaatcc aactttttgc attcgacact tggcatcatc ccggcggcaa
     2281 atgtaagtcg gttgcagacc agcctttcga gagttccgca tgcattcttt acgctgcgtg
     2341 aagattccca aaagccggtc gtatggttgt tgggtccgcg cagtaactca gatattatcg
     2401 agcgcgccgc aaaaagcgcg tatctgaatc cattgagcct gcccgaagaa ttcgatggta
     2461 ccccggctca aattacttca tccatccgaa aggcgattga agcctctaag caaaaaattt
     2521 cggagctgga agcaagtctc agcaagcttt ctaacactca tcggaaagaa ctgaacgagt
     2581 tgttgtggga tgtgcatgtc agccgcgcca tggcagatgc catcgtccgc tttggtcagc
     2641 ttcgtcatac atatgttgtg gttggctggg tgccatccgc tgatctaaaa ttattgattg
     2701 aacgtctcaa acaagcttcc aaggaaatcc tcatcgaggc aattccgacc gaacgttctg
     2761 gccatcactc gaatgtgccc gtagcactgt caacaaaccc ctggctgcgc ccgatacaga
     2821 accttgtcac cacctatggc cgccctgctt atggggagct tgatccgaca cttttggtag
     2881 ccctgagttt tccggttctt tatggcgcga tgttcgggga tttgggacag ggtctggttc
     2941 taatgctggt cggtttcctt gctcataaac ggatttttat gaaaggcttg caaagccttg
     3001 gcttgttgct cgtctactgc ggttttttcg ccacgatctt cggggcatta tacggaagta
     3061 tatttggctt tgaaggacat ttgatcgaag aatacttaca cttccacttc gagcccttat
     3121 ggatgagccc acttgagaac attctgaccc ttcttgggtt cgcgattgac gcaggtatcg
     3181 ttatattgat cttcagctac ctgttaggcc tgttcaatca gtatcgggcg ggtgattggt
     3241 cgcacttctg gtttggtcac accggcgtgg tcgcgttgat cttctacatt tctttcctgg
     3301 cgctgctcgg cggattcctc ggtaccactg caattgcgcc caggattgcg gtggcaatca
     3361 ataacttacc gatcccatgg actttgctgg caggtgtgtt tgggctgggc gtcatgtttt
     3421 ctggtttact ccgaaattgg gcggaaggtc accgtcccct gatcgaaggc aaaggcgtcg
     3481 gcggttttat tatgttcctt gtgcagtcgt tcatggattt gtttgaatct gtgatcagca
     3541 tgttgagcaa caccctatcg ttcgtacgcg taggtgcctt cgccgtggcg catggtggtt
     3601 tgagccttgc attcttctcg ctggctggtg aggaacctga tcttggtttc tggattactc
     3661 tgttgatcgg taacatcttc atcatcggtt ttgaaggttt gatcgtgtat atccaaacga
     3721 tgcgtttgca ctactatgag attttgggca agttcttcca tggtggaggc atgcgcttcg
     3781 agcccctcac tttgaccccc aggaaagagg atgcgtagtt cagaaattta tttatgttta
     3841 tcctacgtcg acgatgtaat aacaaaaatc aatctaaaac cgaaagtata aggagataaa
     3901 caatgttttt agtctttgct gtattagtgg gcttgatccc cgtcattcct gccgtgcttt
     3961 atgtaattca aaacggaaag accagccccg ctcaggccgt ttccaagatg gtgaccggct
     4021 ttaatacctt caacttctta gtgggtatga tggccgccgg tctcggtgtt gtgtggctgg
     4081 catccccttc aggcgctatg gcttccggtt tggcgcagga aggtgtcgtt gatcaatatg
     4141 cgacgctggc tgctgctcta tccacgggtc tggcctgtat tggtgcaggt atcgcggtgg
     4201 gtggctccgg tgctgcagcc gttggcgcaa ccgctgaaaa gcccgagtca ttcggtcgtg
     4261 cgctgatctt cgtaggtctg tctgaaggta tcgctattta cggtttgatc atttccttcc
     4321 tcgtactccc gtaaggatag ggagctgtca tgaaggtttt ggtcatcggt catccggagg
     4381 cagtgttggg tttctcgctc gcaggggtaa gcgggcgagc cgcttcgacc gcggccgagg
     4441 tcaatcaggc gttggacgat gttcaggcct caaaggatgt tggaattgtc cttgtcaccc
     4501 aggatgtggc tgaattgata cctgcccgaa tggaacatct caaactgcgc agcactattc
     4561 cgctggtcgt tgagatcccg tcgccaagcg gcgtgccaga gggccaggcc tctctgggag
     4621 agatcgtctt gcgtgcgatt gggatcaagt tgtaaattgt tttgcctatt cgttgagcgc
     4681 agaaaataga aaagtgccgc ctaaggatct gaaatcggac aagcggtaga ttggatagtt
     4741 attttatgaa aactgaagca caagatattg agatgctggc gcgtgccatt ctgacagaag
     4801 cacgcgagga agctgatcaa ctaaaagcgg acgcacagga aaaagcggat ggaatccgaa
     4861 agcgcgcaca ggaacaggca gatgccgaac gaaaagccat tctggatcgc gctcaggaag
     4921 aggcagagcg cctgcgcggt cagtccgtgg caacggctca attgaaagcg cgttccacac
     4981 agctgacgca tcgagaacaa ttgctcgacc gcgtcttcaa ggctgtgaaa gagaaattgc
     5041 ctgaggttca aaaccgcgca gattatgatg aaattgctgc catgttgctt cgcgaagctc
     5101 tcacgcagtt aagggtccat aaggccaatg tgcgagccga tgaagcaaca ttgaagtctc
     5161 tgaagaaggg cacgttgaga gatatttcga aggaactcaa cggagaattt aaggacggtg
     5221 atgcactgga agaggggacc ggtgttgttg tcgatgcggc cgatggaaaa ttgcactacg
     5281 ataataggct ggagactcgc ttgagccgtt tgcaaggcac attgcgttca gcagtttatc
     5341 aagtgctgac tggagataag gcatgagtga tcaagtagga acaattacct ggattagtgg
     5401 tcccgttgta cgggcacgcg gatcccgtca tgtgggcatg ctcgaactcg tcgaagttgg
     5461 cgcggagcgg ctcgtcggtg aagtgatcgg tcttaaaggc gatcagatga cggtgcaggt
     5521 gtacgaagag accgctggaa tgcagccagg cgcgccgatc tttggcacgg gcctgcctct
     5581 ctccgttcaa ctgggacccg gtctgcttca aagcatttat gacggtgtgc agcgcccact
     5641 tccattgatt gaagcggcaa tgggttcatt tatcgaacgc ggggcgcgct tcgacccaat
     5701 tgaccgctcg aagaagtgga aatttacacc gaaggtcaac gtcggtgatg aggtcaaagg
     5761 cggtcaggtc ctcggcattg tgatggagac ggataccttt gagcatcgcg tgatgcttca
     5821 tccagacgat tctggagaag ttacgtggct tgccgcagaa ggcgaataca cggtcgaaga
     5881 accgattgcc cgcgtcaaaa aaggcaagga agaaaaagaa gttggcatgt tgcagcgttg
     5941 gccggttcgc cgtccgcgtc cgttcgttaa ccggcgcggt gtggatgtgc cattggtcac
     6001 tggtcagcgt attctggata cattcttccc ggttgcgaag ggtggggcag caggtatccc
     6061 tggtccgttc ggatcaggca aaaccgttac ccagcatagc attgcgaaat gggcagacgc
     6121 cgacatcatt gtgtacatcg gttgtggcga acgcggcaac gagatgaccg aggtgctgga
     6181 ggagttcccg aagctggaag acccgcgcac aggcgcgccg ttgatggagc gcacggtgtt
     6241 aatcgccaac acgtcgaata tgcctgtggc cgcgcgtgaa gccagcattt atacgggcat
     6301 cacgattgca gagtattacc gcgacatggg ttacgatgtg cttctgttgg ccgattccac
     6361 ttcgcgttgg ggagaggcct tgcgcgagat ttcaggacgg ttggaggaaa tgcctggtga
     6421 ggagggatac ccggcttatc tcgctgcccg cctcgcggaa ttttacgaac gctctggcag
     6481 ggtgatttgt ctgggtaacc atggcaattc agatgcacaa ggccgtaagg gttccgtctc
     6541 ggtggttggc gcagtttcgc ctcctggcgg cgatttctcg gaacctgtga cccaaaactc
     6601 gatgcgcgtg gcaagcactt tctgggcatt ggattatgac ctctcgcgcc gccgtcactt
     6661 ccccgccatc aactggacga gcagttactc actctacaat tt
//
LOCUS       DASTJP010000163         5945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1125888, whole genome shotgun sequence.
ACCESSION   DASTJP010000163 DASTJP010000000
VERSION     DASTJP010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125888"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFQ23_04990"
     CDS             <1..343
                     /locus_tag="VFQ23_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET9905967.1"
                     /translation="EQVKLDQILGHGGFFKTREVGQRVMAAAMNVPVSVMESAGEGGA
                     WGIALLASYMLHKQENESLESYLSNTVFPGENGITVAPDQKDVDGFAAFMERYQNGLV
                     IERAAVDALKK"
     gene            402..1043
                     /locus_tag="VFQ23_04995"
     CDS             402..1043
                     /locus_tag="VFQ23_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET9905968.1"
                     /translation="MLEKLKEELVELHLELPRNNLVVWTGGNVSARDPETGLVVIKAS
                     GIRYEEMGPQHMVVVDIDGTMAEGDYKPSSDVYSHLYIYKHRQDVNGVVHTHSAYATA
                     FAAVNKPIPVVLTAIADEFGGAIPCGGFALIGDDAIGKVVVESIGTSPAVLLKNHGVF
                     TIGKDAKSAVKAAVMTEENARTVWLAMQIGVPDEISQEDVEKLHHRYTHVYGQ"
     gene            1092..2603
                     /gene="araA"
                     /locus_tag="VFQ23_05000"
     CDS             1092..2603
                     /gene="araA"
                     /locus_tag="VFQ23_05000"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HET9905969.1"
                     /translation="MIDLKQYEVWFVTGSQHLYGPKTLEQVAANSVEIANALGGSAEI
                     PVQVVFKPVLTTSEDIRGLCLQANSTMNCVGLITWMHTFSPAKMWIAGLSLLKKPFLH
                     LHTQYNREIPWADIDMDFMNLNQSAHGDREFGFIGSRMRLNRKVVVGHWQDIDVQKSI
                     GVWIRAACAWADAQGAKIARFGDNMRDVAVTEGDKVEAQIRLGYDVYGYGVGDLVKYV
                     DEVTNAEVDQLVQSYLDEYDVAAELKPGGTKNSSLCDGARIEIGLRNFLSAGNFKGFT
                     TTFEDLHGLVQLPGLAVQRLMKDGYGFGAEGDWKTSALVRAMKVMNDGLNGGVSFMED
                     YTYHFNGAGDKVLGAHMLEICESIAAEKPRLELHPLSIGGKADPVRLIFDSQTGSAIC
                     ASIVDMGQRFRLLVNQVDVLPTDAPLPKLPVARALWIPRPNLKVAAAAWIYAGGAHHT
                     GFSFSVTPEHLQDFADIAGIEFLLIDEETRISTFKERLRWNDLYYHLSNGLNV"
     gene            2687..3661
                     /locus_tag="VFQ23_05005"
     CDS             2687..3661
                     /locus_tag="VFQ23_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9905970.1"
                     /translation="MDYAEIPGINKAVARVVQGSTMIGSDLDEAKSFALLDQVFDMGC
                     NTLDTAHGYSKGESERIIGRWIQSRGLREEVVIITKGAAHSDDRRRMTPFDIASDLHD
                     SLARLKTDYIDLYLLHRDDPNVPVEPIMDSLNEHRSAGRFRAFGASNWSHQRISEANA
                     YAVANGLEPFVASSPQFSLAESLDEPWPLCISISGPAGATAREWYAQTQMPLLAWSPL
                     ASGFFSGRFRRDNLHSFGEREWDEVCVRTYANEANFQRLDRAEIVAAEKGLTAAQVAL
                     AYIMNQPMNVFALVGPHSGEKFRMNVQASELRLTQEEMAWIDLRTDSR"
     gene            3700..4584
                     /locus_tag="VFQ23_05010"
     CDS             3700..4584
                     /locus_tag="VFQ23_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905971.1"
                     /translation="METVNFLGHECYSLSNEFIRLLVTRSIGPRILSFGFADGENLFA
                     ELPDFVTDCPGNGVFHFYGGHRLWHAPEEPRRTYLPDDSPVHITPLENGFVVTQNLEH
                     QTGLQKSMEITISGTSAQVLVKHHLTNHGLWDVTCAPWAITQFKTGGMAILPQSREET
                     GVLPNRSITLWPYTEMSDPRVQWGRDFILIDANMDAPFKVGFPNPRGWLAYWINGVLF
                     VKRATYKAGSQYYDFGSSSECYCNDAFLELETLAPINRIEPGGTATHVETWGLYKDID
                     RPRDAREVQSLVERLALE"
     gene            complement(4648..5463)
                     /locus_tag="VFQ23_05015"
     CDS             complement(4648..5463)
                     /locus_tag="VFQ23_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9905972.1"
                     /translation="MLLTKHKTNDGSRWAVDEHFLLQGLTLTKLLEMPRGEMLQALEN
                     ASKNEIASGDEEAPIDSHQEVWAAGVTYLRSRDARKAESTVADMYEKVYEAERPEIFT
                     KSIGWRVSGPGAPIRIRRDSRWNVPEPELVLVINCYNEIVGYCAGNDVSSRDIEGENP
                     LYLPQAKIYNGSCALGHGVLICEPAAIKDIPIRLDIQRVNEIVFSGEANTSTMKRTLP
                     ELVDYLTRELDFPQGVFLMTGTCLVPDSNFALQIDDVVRIQVGEMKIENRVQS"
     gene            complement(5463..>5945)
                     /locus_tag="VFQ23_05020"
     CDS             complement(5463..>5945)
                     /locus_tag="VFQ23_05020"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9905973.1"
                     /translation="PVKAGDIIALIGCGPMGTGMEETYQLTSALKYLPHGKSIALITD
                     ARFSGVSTGACIGHIGPEALAGGPLGKLQDGDVVQIIVDCVNLSGQINFVGDQDRRFN
                     SDEGAKILANRQPHSQLQPNPKLPDDTRLWAMLQNISGGIWGGCVYDVEKIMEHLGGA
                     "
BASE COUNT         1492 a         1401 c         1546 g         1506 t
ORIGIN      
        1 ggaacaagtg aagctcgatc aaattctcgg tcacggtggc ttcttcaaga caagagaggt
       61 ggggcaaaga gtgatggcag ccgcgatgaa cgtccccgtt tcagtgatgg agagcgcagg
      121 ggagggcggc gcctggggaa ttgcactcct tgcatcctat atgctccata agcaagagaa
      181 tgaatccttg gagtcttacc tctccaatac ggtctttccg ggtgaaaacg gaataacggt
      241 cgcacccgat caaaaggatg tcgatggctt tgccgctttc atggagcgtt accaaaatgg
      301 gcttgtcatc gaaagggccg cggtggatgc gctgaagaag taaacaaacc gaacgcccgt
      361 cccttttagg gatggaagca tgggttaatt tggagaacta tatgcttgaa aaactcaaag
      421 aagaattggt tgaattacac cttgaacttc ccaggaataa cctggtggtg tggacaggtg
      481 gcaatgtcag cgcccgcgat cccgagacag gactcgtggt gatcaaggca agcggaatcc
      541 gatatgaaga gatgggtcca cagcacatgg tagtggtgga tatagacggc acaatggccg
      601 aaggcgatta caaaccatct tccgatgtgt acagccattt gtatatttat aaacatcgtc
      661 aggatgtgaa tggggtggtg cacacacatt cggcgtatgc gacggcgttt gccgcggtga
      721 acaaacccat ccctgttgtg ttgacagcga tcgctgatga atttgggggg gccattccgt
      781 gcggcggatt tgccttgatt ggcgacgatg caatcggtaa agtggttgtg gaaagtattg
      841 gtacatcccc tgcggtgttg ctcaagaatc atggtgtgtt tacgattggc aaggatgcga
      901 agtctgcggt caaggctgca gtaatgacag aagaaaatgc caggacggtc tggcttgcga
      961 tgcagatcgg cgtccctgat gaaatatcac aggaagacgt ggagaaacta catcatcgct
     1021 acacgcatgt ctacgggcaa tgaatatttt aaggacgagg tctccccgtc ccgcctttaa
     1081 ggagcaaaat catgattgat cttaaacaat acgaagtctg gtttgtaacg ggtagccaac
     1141 acctgtatgg tcccaaaacg ctggaacaag tggcagccaa ctcggttgaa attgcaaacg
     1201 cattaggtgg ctcggctgag attccggtgc aggtagtgtt taagcctgta ttgacgactt
     1261 cggaagatat tcgtggcttg tgcctgcaag ccaattccac aatgaattgc gtgggtctta
     1321 ttacctggat gcataccttc tcgcctgcca aaatgtggat tgccggtttg agcctgctca
     1381 agaaaccctt cctgcatttg catacccaat ataaccgcga gatcccatgg gctgacattg
     1441 acatggactt tatgaacctg aatcaatcag cacatggtga ccgtgagttt gggttcattg
     1501 gcagtcgcat gcgacttaac cgcaaagtgg ttgttggtca ttggcaggac atcgacgttc
     1561 aaaaaagcat aggagtctgg atccgtgcag cctgcgcgtg ggcagatgca caaggtgcaa
     1621 agatcgcgcg cttcggcgac aatatgcgcg atgttgctgt gaccgaaggt gacaaagttg
     1681 aggcgcaaat tcggcttggc tatgatgtct atggatatgg ggtaggagat ttagtcaaat
     1741 atgttgatga agtcacgaat gccgaagtgg atcaacttgt gcaatcatac ctggatgaat
     1801 acgatgtggc tgctgaactc aaacccgggg gtacaaaaaa ttcatctctt tgtgacggag
     1861 cacgaatcga aattggcttg cgtaacttcc tgagtgccgg caatttcaaa gggttcacca
     1921 ccaccttcga agatttgcat ggactggtac agttgcctgg gctggctgtc cagcgtttga
     1981 tgaaggacgg ttatggcttc ggtgcagaag gcgactggaa gacgtccgcg ttagtacgcg
     2041 ccatgaaggt tatgaatgac gggctaaacg gtggtgtcag tttcatggag gattatacat
     2101 atcacttcaa tggagcaggc gataaggtgc tgggcgcgca tatgcttgag atttgcgaat
     2161 cgattgccgc tgagaagccc cggcttgagc ttcatccact ctccatcgga gggaaagcag
     2221 atcctgtccg attgattttc gattcacaga ccggttccgc catctgcgca tccatcgtgg
     2281 atatggggca gcgattccgg ttattggtga atcaggtcga tgttcttcca acggacgcgc
     2341 cgctgccgaa gctgccggtt gcgagggcgt tatggattcc acgtccgaac ttaaaggttg
     2401 cagccgccgc gtggatttac gccggaggag cacatcatac gggatttagt ttctctgtca
     2461 cgcccgagca tcttcaggac tttgcagaca tcgccggaat tgaatttttg ctgatcgatg
     2521 aagagacccg cataagcacc ttcaaggaaa gactccgctg gaatgatctg tactaccatt
     2581 tgtccaacgg cttaaatgtt tgaagtgttt atacttagta ctcctaacta aagtcgctgg
     2641 tcagtcatga ccactaccat gcagacttaa ggcagtgaaa tacgatatgg attacgcaga
     2701 aatccctgga ataaacaaag ctgtagcgcg cgttgtgcag ggatcaacga tgatcggaag
     2761 tgatctggat gaggcaaaga gttttgcatt actggatcag gtttttgaca tgggctgcaa
     2821 cacccttgat acggcccatg gttacagcaa gggcgagagt gagcgcatta tcggccgttg
     2881 gatacagtcc aggggtctgc gtgaggaagt cgtcatcatt acgaagggcg cggctcattc
     2941 ggatgatcgc cgaaggatga cgccctttga cattgcttct gacctgcacg attccctggc
     3001 ccggctcaag accgattata tagatttata cctgttacac agagatgatc ccaatgtgcc
     3061 cgttgaacca atcatggact cattgaatga acatcgaagc gcaggtcggt tccgcgcctt
     3121 tggcgcttcg aattggtcac accagcgcat ttcggaagcc aatgcctatg ctgtggcaaa
     3181 cggacttgag ccgtttgtag cgagtagtcc gcaattcagc ctcgcggaat cgcttgacga
     3241 gccatggccg ttgtgcatca gtataagtgg ccctgcaggt gcaacagcac gcgagtggta
     3301 tgcacaaaca caaatgccat tgctggcctg gtcgccgctg gcgagtggtt ttttttccgg
     3361 aagatttcgt cgtgacaatc tgcattcatt tggcgagagg gagtgggatg aggtctgcgt
     3421 gagaacgtac gcgaatgagg caaacttcca gcgtttagac cgggccgaga tagtagccgc
     3481 agaaaagggc cttaccgctg cccaggtagc attagcatat ataatgaatc agcccatgaa
     3541 tgtatttgca ttggtagggc cgcacagcgg cgagaagttt aggatgaacg tccaggctag
     3601 cgagcttcgg ttaactcagg aagaaatggc ttggatcgat ctaagaacgg atagtcgctg
     3661 aaatattgag gtttgtgatt gaaaagagag ttacagataa tggaaacagt taatttcctg
     3721 ggtcatgaat gctattcact atcaaatgag ttcatacgct tgcttgtgac ccgctccatt
     3781 ggaccgcgta ttttatcctt cgggtttgca gatggtgaga acttattcgc cgaattgcca
     3841 gactttgtga ctgattgccc tggcaatggt gtattccact tttatggtgg gcatcgcctg
     3901 tggcatgcac ccgaggaacc tcgccgtact tatctgccag atgattctcc ggtccatatt
     3961 actcctcttg aaaacggttt tgtagtgaca caaaaccttg agcatcaaac cggattgcaa
     4021 aaatcaatgg agataactat ttcgggtacc tcggctcagg tactggtcaa acatcatctc
     4081 acaaatcatg gtctctggga tgtgacctgc gctccctggg caattaccca attcaaaacc
     4141 ggtggcatgg cgatattgcc ccaatcacgt gaagaaacag gagtgttacc gaatcgttct
     4201 ataacgctat ggccgtatac tgaaatgtcc gatccgcgtg tgcaatgggg gcgtgatttc
     4261 attctcatcg atgcgaacat ggatgcacca ttcaaggttg gttttcccaa cccacgcggc
     4321 tggctggcat actggatcaa cggagtgctt ttcgtaaagc gcgcgacgta taaggcaggc
     4381 tcacagtatt acgatttcgg cagttccagt gaatgttact gcaatgatgc gtttctggaa
     4441 ttggaaacac tggcacctat taataggata gagcccggag ggacggctac acatgttgaa
     4501 acctggggct tgtacaaaga tattgatcgc ccccgcgatg ctagagaggt acaatctctg
     4561 gttgaaagat tggcactgga gtaaagaccc aatgcttatt ttcttggcat ggtctgattt
     4621 caaagaagaa acccgcagac tcttccttca agactgaacc ctgttctcaa ttttcatttc
     4681 cccaacttga atacggacaa catcgtcaat ttgcagtgcg aaattactgt ctggcaccag
     4741 gcaggttccg gtcattaaaa ataccccctg cgggaaatcc aattctcggg tgagataatc
     4801 aaccagctcg ggcaatgttc gtttcatggt ggatgtgttt gcttcgccac tgaatacgat
     4861 ttcatttaca cgttgaatgt ccaatcgaat tgggatatct ttgattgcag ccggctcgca
     4921 aatcaaaacg ccatgaccta atgcacatga gccattatag attttcgcct gtggcaaata
     4981 gagcgggttc tcaccttcaa tatcacgcga tgagacatcg ttccctgcgc aataaccgac
     5041 gatctcgtta taacaattaa tcactaatac caactctggt tccggcacat tccaccggct
     5101 atccctgcgg attcgaatcg gcgctccggg gcccgataca cgccagccta ttgacttcgt
     5161 aaaaatttca ggccgttccg cctcatagac tttttcatac atatctgcca ctgtggattc
     5221 tgccttgcgc gcgtcgcgac ttcgcaaata ggtgactccc gccgcccaca cttcctgatg
     5281 tgaatcaata ggcgcctctt cgtcaccgga ggcaatttca ttctttgatg cattttccaa
     5341 cgcttggagc atttctccgc gcggcatctc aagcagtttg gtcagggtta atccttgcaa
     5401 aaggaaatgc tcatctactg cccaacgtga tccatcgttt gttttgtgtt ttgtaagtag
     5461 cattacgcac ctcccaggtg ttccattatt ttttcaacat catacacaca accgccccat
     5521 attccaccgc tgatgttttg cagcatggcc cacaagcgcg tgtcatccgg caactttggg
     5581 ttcggttgca actgcgaatg aggctgacga tttgctaata tcttcgcccc ttcatcagaa
     5641 ttaaaacgtc tatcctggtc accgacgaaa ttgatttgcc cggaaagatt aacacaatct
     5701 acaatgatct gaaccacgtc tccatcctgc aatttcccca gtggaccacc tgccaatgct
     5761 tccggaccga tgtgaccaat acatgcccct gtgctaactc cgctaaagcg tgcatcggtg
     5821 atcaacgcga tggatttccc gtgtggtaaa tatttcaaag ctgaggtgag ttgataggtc
     5881 tcttccatgc ctgtgcccat gggtccacaa ccgatcagcg caataatatc tcctgctttc
     5941 acagg
//
LOCUS       DASTJP010000164         4098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_181789, whole genome shotgun sequence.
ACCESSION   DASTJP010000164 DASTJP010000000
VERSION     DASTJP010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181789"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFQ23_05025"
     CDS             <1..449
                     /locus_tag="VFQ23_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905974.1"
                     /translation="SQSSVEIRESLAPVIAVTDTVLIPVAPTPQQFSDFAPRDGWRYR
                     LVKMFDRFFRWGQIQANNPDAVNGPWFGAFCHTVCLATELLWSAEERAAWENQNKQLA
                     ENIVHTIQRDTGRRVLVAVQCQRLHHLIPLLRARSDLFDIVGYQYL"
     gene            485..1177
                     /locus_tag="VFQ23_05030"
     CDS             485..1177
                     /locus_tag="VFQ23_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET9905975.1"
                     /translation="MPNVITKIINFLLVLLIPILVVLGSARLLATDTYLALEYGKTSF
                     PPDVYGFTSQQRFELASVNVHYVRAHLPDDALATQKLDATLIYSPRELSHMADVQSVF
                     QVIVSVWQVAFVLLLFLGLILWATGEHRQLAAAVQSGGLFTSAMILTIALLAIFAWQS
                     WFSIFHLFFFQPGSWLFSYSDTLIRLFPVNFWFDATLTISVLSLMGGLSLAWMGRQWR
                     RSFQKSPYIIKL"
     gene            complement(1248..1841)
                     /locus_tag="VFQ23_05035"
     CDS             complement(1248..1841)
                     /locus_tag="VFQ23_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9905976.1"
                     /translation="MAKTDRRVERTREQLQKALIELIAERGYDAITIQDIVDRANIGR
                     TTFYLHYNSKDELFMSCHEAIVGKFHIGLLHALSKEELLSSDVPSEVSLAYRHLEEER
                     SLLYPVFQGKDSQLILRRIRDRSAREIESNLRTVFADVESNIPLDILANYLAGAQIAL
                     MQWWLEKRRPHIPGDLTETLYRLQRAAIRDAFGIQGG"
     gene            2226..2438
                     /locus_tag="VFQ23_05040"
     CDS             2226..2438
                     /locus_tag="VFQ23_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905977.1"
                     /translation="MAENQKEPSRGSPPGMPRWLKLIIIVFIVLVAAVAVLHLMGFGF
                     GSHGGINSAVMLARVQINHVILKGKF"
     gene            2446..2982
                     /locus_tag="VFQ23_05045"
     CDS             2446..2982
                     /locus_tag="VFQ23_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905978.1"
                     /translation="MKPGLRKFVRTIHVTVTVGWIGAVAGFVALALTGLTSQNAQTVS
                     AAYISMDLITRLVIVPLSLVPLFLTGPLLSLGTPWGLFRHYWIIVKLVINVLSTIILF
                     VHLEPISYLARVAAAGTLSHDDRSLQIQVVVAAGAGLLALLAATALAVYKPRGMTSYG
                     WRKQYRERTSSSDVDTTG"
     gene            complement(3005..>4098)
                     /locus_tag="VFQ23_05050"
     CDS             complement(3005..>4098)
                     /locus_tag="VFQ23_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET9905979.1"
                     /translation="PTAKPATAIKRVGYVDIAKGIGIILVVMAHNDFALISPFAHKLI
                     YSFHMPMFFFMSGMFFKPDMPFWSFVRQRFHRVLKPFFVMLLLIYFASLSFSKVSFVM
                     ASRRLLKAMYASGQYLDWVQLWFLPHLFVVSLFAYLLIRVLRHQWLFKVRWLMLITVY
                     IVGVLSINVFSPIEFNLLGKELNLYGLPFSIDLVLVTGFFFILAYELNQHPYASSLES
                     RWVLLISGVLLVFLVWYFPYKIDFNIRQFDSLPINTLEALLGILFILAISRQLERIGV
                     LSSAFSYIGQASLVILIFQVPIQDYWGQKLLAVTDNLAFSYWVSFLVGVIGPIIINAL
                     FIRPNSIVKEWFGQTAPRETKQSAVSVFE"
BASE COUNT         1063 a         1009 c          957 g         1069 t
ORIGIN      
        1 tctcgcagtc ttctgtggaa attcgtgagt ccctggcacc cgtcattgca gtaacagata
       61 cagtgctgat tccagtcgca ccgactcccc agcaattctc tgattttgcc cctcgcgatg
      121 gatggcgata tcgtctcgtg aaaatgtttg accgattttt tcggtggggg caaattcaag
      181 ccaacaaccc ggacgcagtc aatggaccat ggttcggcgc gttctgtcac acggtttgtt
      241 tggcaacgga gctgctatgg agtgcagagg agcgcgccgc ctgggaaaac caaaacaagc
      301 agctagcaga aaatatcgtg cataccatcc agagggacac tgggcgacgt gtgctagtgg
      361 ctgtacaatg ccaacgcttg caccacctga ttccgctact tcgtgcgcgt tccgatctat
      421 tcgatattgt ggggtatcaa tacttgtaaa gtaacgctcc cggctcccat tcaaaggtta
      481 atccatgccg aatgtcatca ccaagatcat aaatttcttg ctcgtgctcc ttattccaat
      541 ccttgtcgta cttggttcgg cgcggctgtt ggctacggac acctacctgg cgcttgagta
      601 cggcaaaacg agcttcccac ctgacgtata cggttttact tcgcagcaac gctttgaact
      661 tgcatccgtc aatgttcatt atgtgcgagc tcatctgcca gatgatgcac tcgcgaccca
      721 gaaattggat gcaaccctga tctatagtcc ccgcgaactc tcacatatgg cggatgttca
      781 atcagtattt caagtgattg tgagcgtctg gcaagtcgct tttgttctgc ttctcttttt
      841 gggactcatc ttgtgggcaa ctggcgagca cagacagctt gccgcggccg tccaatccgg
      901 aggcttattc acttctgcca tgatcctgac gattgccctg ctcgcgattt tcgcctggca
      961 atcctggttt agtatattcc atctattttt cttccagcca ggatcttggc tgttctccta
     1021 ttctgatacc cttattcgcc tgtttccggt gaatttctgg tttgacgcca ctctaacaat
     1081 atcggtcctc agcctaatgg gagggctctc tttagcctgg atggggcgcc aatggcgacg
     1141 ttcgtttcag aagtctccat atataataaa actatagctt gcttttagtc cccagacaga
     1201 tcacaggcta tcgagatgat agcctgttga ttcggttttc agatctgcta tccgccttgg
     1261 ataccaaacg catcccgaat agcggcacgc tgaaggcgat acagggtctc cgtcaagtca
     1321 cctgggatgt gcggacgtcg tttttccaac caccattgca tcagcgcgat ctgggcccct
     1381 gcaagatagt ttgccagtat atccagcgga atattacttt ctacgtctgc aaagacagtc
     1441 cgcaggttgg actcaatctc tcgcgcactc ctgtcacgga ttcgacgaag gattagttgg
     1501 ctgtcttttc cttgaaacac aggatataac agcgatcttt cttcttcaag atgcctgtaa
     1561 gccaagctta cttcagatgg tacatccgac gataaaagct cttccttaga aagagcgtgc
     1621 agtagtccaa tgtgaaactt gcccacaatg gcttcgtggc agctcataaa taattcgtct
     1681 ttgctgttgt agtgcaaata gaatgtagtg cgcccgatat tggcacgatc gacaatatcc
     1741 tgaatcgtga ttgcatcata gccacgctcc gcaatcaatt cgatcaaagc cttttgaagt
     1801 tgttcgcgcg tacgctccac tcgccggtca gtctttgcca taatgcaacc tctcttaaga
     1861 acatggactc ttcacagaca actacggtgg aggcattggt gaatactttt caacacgagt
     1921 caatagcatg ttgcgcttgt tgaaaaagca ttacggaacg cttacctggt gtcttatcgg
     1981 tttcgaatta tacaagcaga agtagagcga aacgacatcc accatgtacg tagattgacg
     2041 tcatttgcgt ctttcaggcg cgcatgtata cgtctcgact caaacctgtg ttgaataaga
     2101 ttttgtgaac aaatagctta gactgttcat aaatgaacaa atgtcccggg ctgcttattg
     2161 accaagcttt cgaaagacca tacgattgca taatctatct caagcgtatc attggaggaa
     2221 aagtaatggc ggaaaatcaa aaggagccca gccgcggctc accccctgga atgccccgtt
     2281 ggctcaagct aatcatcatc gttttcattg ttttggtcgc tgcagttgcg gtcctgcacc
     2341 ttatgggttt tggctttggc agtcatggtg gaatcaatag tgcagtaatg cttgcccgcg
     2401 tacaaattaa ccatgtgata cttaaaggca agttctagta caccgatgaa acctggtctt
     2461 cggaagtttg ttcgcactat tcacgttact gtcacagttg gctggattgg ggcggtcgcc
     2521 ggctttgtag cgctcgctct tacaggtcta accagccaga acgcccagac cgtgagcgct
     2581 gcttatatct caatggatct catcacccgc ctggtgatcg ttccattaag ccttgtgcca
     2641 ctgttcctga ccggccccct tttatctcta ggaacaccgt ggggtttgtt ccgccactac
     2701 tggatcatcg tgaagctcgt cattaatgtc ctttccacca tcatcctttt cgttcatcta
     2761 gagccaatca gctatctagc acgggttgct gcagccggaa ccttatcaca tgatgatcgt
     2821 agcttgcaga tccaagtggt cgtcgcggct ggtgccggtt tattggcatt actcgcggcc
     2881 acggcactgg ctgtttacaa gccacgcggt atgacctctt atgggtggcg aaagcaatat
     2941 cgagaacgga catcttcatc cgatgtagac accaccgggt gatattcatt gacttgtaag
     3001 gtgtttattc aaacacacta actgctgact gcttggtttc acgcggtgct gtttgaccaa
     3061 accattcctt cacgatcgag tttggacgga tgaatagtgc gttaatgatg atggggccaa
     3121 taacgcccac cagaaaagat acccagtacg agaacgccaa gttatctgtc accgccagca
     3181 gtttttgtcc ccagtaatct tgaatgggaa cctggaaaat caggatgacc aacgatgcct
     3241 gaccaatgta actgaatgcg gatgagagaa caccgatccg ttccaactgc cgggagatcg
     3301 cgaggataaa cagtattccc aatagggctt ccagggtgtt gatcggcagc gaatcaaact
     3361 ggcgaatgtt gaaatcaatc ttatagggaa aataccagac caagaaaacc agtaaaactc
     3421 cagaaatcaa tagcacccag cgactttcaa gagaggaagc gtaaggatgc tgatttagtt
     3481 cgtaggccag aatgaagaaa aagccagtga ctaatacaag gtctatgcta aatggcaaac
     3541 catataaatt gagttctttc cctaaaagat tgaattcaat cggtgagaaa acgttgatgc
     3601 tcagtactcc gacgatatag acagtaatta acatcaacca gcggactttg aaaagccatt
     3661 gatggcgaag gacccgaata agtaggtaag caaagaggct gaccacaaat aagtgcggaa
     3721 gaaaccatag ctggacccaa tccaggtact gtccactggc atacatggcc ttgagaagac
     3781 gtctacttgc catgacgaaa cttaccttcg agaaagataa gctggcaaag taaatcagca
     3841 agagcatcac aaaaaaaggc ttgaggaccc tgtgaaaacg ctgacggaca aagctccaga
     3901 aaggcatgtc aggcttaaag aacatgccag acatgaaaaa aaacattggc atgtggaagg
     3961 agtaaatcag tttgtgtgcg aagggggata tgagtgcaaa atcgttatgc gccattacaa
     4021 ctagaatgat gccaattccc ttggcgatat ctacataccc aactcgctta attgcagtag
     4081 ccggtttcgc agtcggct
//
LOCUS       DASTJP010000165         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1503156, whole genome shotgun sequence.
ACCESSION   DASTJP010000165 DASTJP010000000
VERSION     DASTJP010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503156"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFQ23_05055"
     CDS             complement(<1..566)
                     /locus_tag="VFQ23_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET9905980.1"
                     /translation="MSTNNHADGKYVLEAHDLDIYYGTFRAVTSVNMQVERNKITAII
                     GPSGCGKSTLLRSFNRMNELYAGTSVKGKITFNGEDIYAREVDPVEVRYRIGMVFQKP
                     NPFPKSIYENIAWTARIHGFKGNMDELVENSLRQAAIWDEVKDKLHQSGLSLSGGQQQ
                     RLCIARAISIKPEVILMDEPASALDPIAT"
     gene            complement(569..1810)
                     /gene="pstA"
                     /locus_tag="VFQ23_05060"
     CDS             complement(569..1810)
                     /gene="pstA"
                     /locus_tag="VFQ23_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9905981.1"
                     /translation="MKAKTIAQGFTDNLNSRNRRGVIWHALFQASTIVGIVALIALML
                     NIINGAFGYVAYEARVDPDTLTVDGVPVQEQNKEQLVALLQSKISSGAFRTLEKEKPF
                     ADRTREDVYQLIIDRVIRPDIVSVWNLWSSLTRADEIRATVAEENPEAELDFVSWLTT
                     DFIARPQSSEPLTAGVRTAVLGSLWTILFTILLAFPIGVGAAIYLEEYASDNWLNRII
                     QTNINNLAGVPSIIYGILGLAIFVRTLEPLTSGELFGLVDPTTASGRTVLSAGLTLGL
                     LVLPLIIINSQEAIRAVPRSLRNASFGVGATKLQTVWSHVLPNAMPGILTGTILAVSR
                     AIGETAPLVVVGASTAISFDPSSPFSKFTTLPIQIYQWTSRPQDEFRSLAAAAILVLL
                     ILLLSLNASAILLRNRFSRRF"
     gene            complement(1807..2709)
                     /gene="pstC"
                     /locus_tag="VFQ23_05065"
     CDS             complement(1807..2709)
                     /gene="pstC"
                     /locus_tag="VFQ23_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET9905982.1"
                     /translation="MNSLRRKPRWGETIVQLLLFFSGFFSIFTTVGIVYELGKEALLF
                     FQMPEVNLLDTITTTEWIPHTGKFGIWPLFTATLMTTAIAMLIAIPLGMAVAIYLSEY
                     AKPRVRSTLKPILEVLAGIPTIVYGYFALTFMTPILRQLFGSNTVQIYNTASAGLVMG
                     ILILPLISSMTEDALSAVPRPLREAAYGVGGTKLEVAIQIVVPAALSGIAAAIIVGIS
                     RAIGETMIVAVAAGAGPNFTLNPFIGAETITGHIVRISGGDLSYDSIDYNSLFALGLF
                     LFFVTLTLNMISQAIVRRFREVYE"
     gene            complement(2864..3931)
                     /locus_tag="VFQ23_05070"
     CDS             complement(2864..3931)
                     /locus_tag="VFQ23_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9905983.1"
                     /translation="MTKFVRSFMFVFIVMSLLLAACAPATTEAPAPTEVPPTEVPATD
                     TPVTEAPAEDPMAMYAADAVSGDIIAAGSSTVFPLAERMKQRFEEEGFAGNLTIDSIG
                     SGAGIERFCVNGETDIANASRAIRDTEIESCAALTPPRTPIEFQVGIDALAVVVSSEN
                     DFATDVTTEELAALYSTATNWSDVRPEWPTEPILRFSPGTDSGTFDYFVEAVMTPALD
                     DDATEDVDEGHDAILNSAGTQFSEDDNVLVQGVEGSPYAIGYFGYAYFQENQGALKAL
                     SINGIEPNAETAESGEYPISRPLFIYSDATILQEKPQVASFIWFFLNNVNNEITDVGY
                     FPVSDAKLQEAKDAWLAGVGE"
BASE COUNT          982 a          989 c         1090 g          921 t
ORIGIN      
        1 gtggcaatcg ggtccagagc ggaggcgggt tcgtccatca gaatgacttc gggtttgatg
       61 gaaatcgccc gcgcgatgca caagcgttgc tgctgaccac ccgagagcga taacccggat
      121 tggtgcagtt tgtcctttac ttcatcccag atcgcggctt gccgcagtga gttttccacc
      181 aattcatcca tattgccttt gaagccgtgg atgcgggccg tccaggcgat gttttcatag
      241 atgctcttgg ggaacgggtt gggcttttga aagaccattc cgatccggta acgaacctcc
      301 accgggtcca cctcccgggc gtagatatcc tcgccgttaa aagtgatttt ccctttgacg
      361 gaagttccgg catatagctc gttcatacgg ttaaaagaac gcagcagcgt tgatttgccg
      421 cagccagaag gaccaataat cgcggtgatc ttattgcgtt ctacctgcat gttgacgctg
      481 gtcacggctc tgaaagtgcc gtagtaaata tcaaggtcat gcgcctcgag aacgtattta
      541 ccatctgcat ggttgtttgt agacattctt agaatctcct gctgaagcgg ttgcgtaata
      601 ggatggcaga tgcgttcagg ctgagcaata agatcaaaag cacaagaatg gctgccgccg
      661 caaggcttcg gaattcatcc tgcggacggg acgtccactg atagatttga atcggaaggg
      721 tcgtaaattt tgaaaaaggt gaacttgggt caaagctgat ggcagtggaa gccccaacta
      781 ccaccagagg cgccgtctca ccgatagcgc gagaaacagc aaggatcgtg ccagtcagaa
      841 tgcccggcat cgcgttgggt aacacatgag accagaccgt ctgtaatttg gtggcgccga
      901 ccccgaagct ggcattgcgc agtgatcttg gtaccgcgcg gatcgcctcc tgggagttga
      961 tgataattag aggcagcaca agcagcccga gtgtgagccc agccgaaagc accgttcgac
     1021 cactcgctgt ggtcgggtca accagcccaa acagttcacc gctggtgagc ggttcaaggg
     1081 tgcgaacaaa aatcgcgagt ccgaggatcc cataaatgat ggatggcacc ccagccaggt
     1141 tattgatgtt ggtctggatg atgcgattca accagttatc agaggcatat tcttccaaat
     1201 aaatagccgc tcctacgccg atggggaacg ccagcagaat tgtgaacagg atggtccata
     1261 aggagccgag aaccgcagtg cgtacaccgg ctgtcagagg ctcactgctt tgagggcgag
     1321 cgatgaagtc tgttgtgagc caggaaacaa aatccagttc cgcttccgga ttctcctccg
     1381 ccaccgttgc acgaatttcg tctgcccggg tcagagacga ccataagttc cacacgctga
     1441 caatatcggg ccggattacc cggtcaatga tgagttggta tacatcttcg cgggtacggt
     1501 cagcgaaggg cttttctttt tccagtgttc gaaaagcccc gctcgaaatt ttagattgaa
     1561 ggagggccac caactgctcc ttgttttgct cctgcacagg cacgccatct acagtcagag
     1621 tatccgggtc aacccgggct tcgtaggcaa cgtatccaaa cgcgccattg atgatattca
     1681 gcatcagagc gatcagtgca acaattccaa caatggtaga agcctggaaa agcgcatgcc
     1741 agatcacacc cctgcgattg cgtgaattga ggttatcggt aaacccttga gcgattgttt
     1801 ttgctttcat tcgtatacct cgcggaagcg gcggacgatg gcctggctga tcatattgag
     1861 ggtgagcgta acaaagaaca gaaacaaccc aagcgcaaag agactgttgt aatcgatcga
     1921 gtcgtaactg agatcgccgc ccgagattct cacgatgtgc cctgtgatcg tctccgcgcc
     1981 gataaatgga ttcaaagtga agttgggtcc ggcaccggcc gcgaccgcaa ctatcatggt
     2041 ctcaccgatg gctcgcgaga tgcctacgat gatggcagcg gcaatcccgg agagcgccgc
     2101 cggaactaca atctgtatgg cgacttccag tttggtcccg cccacgccat aggcagcttc
     2161 ccgcagcggg cgcgggacgg cgcttaatgc atcttcagtc atcgaagaaa ttagcggcaa
     2221 aatcagaatg cccatcacca gccctgccga cgcagtattg taaatttgaa ccgtattact
     2281 accgaagagc tggcgcagga tgggcgtcat gaaggttaag gcaaagtatc cataaacaat
     2341 ggttgggata cctgccagca cctccaaaat tggcttgagc gtactgcgca ctctcggttt
     2401 ggcgtactca cttagataaa ttgccactgc catgccaagc ggaattgcaa tcagcattgc
     2461 aatggctgtt gtcatcagtg ttgcagtgaa aagaggccag ataccaaact tgcccgtatg
     2521 tggaatccat tccgttgtgg taattgtgtc caggagattt acctccggca tctggaaaaa
     2581 gagcaaagcc tcttttccca attcataaac aatacccaca gttgtgaaga tagagaagaa
     2641 accgctgaag aacaacagca actgaacgat ggtttcaccc cagcggggtt tacgacgaag
     2701 actgttcaca gatgctttcc tctgaaaagc tttctcaagc gagagatctg ccatgaaagc
     2761 accttccttt caaataaaat catgagagta attacgacaa attaccttcc ctcctcattt
     2821 gtgagaaggg aaggctgaat atgatgcttc gtaatgcggt tggttattcg cctacacctg
     2881 caagccatgc atctttggct tcctggagct tggcatcaga aactgggaag tacccgacat
     2941 cagtgatctc gttgttgacg ttgttgagga agaaccagat gaaggaagcg acctggggtt
     3001 tttcctgcaa aatggtggca tcggagtaga tgaagagtgg acgggagatc gggtattcac
     3061 cagactcggc ggtttcagcg tttggttcaa taccgttgat cgagagagct ttcaacgctc
     3121 cctgattttc ctggaagtag gcatagccga agtaaccgat ggcataaggt gaaccttcca
     3181 caccctggac gaggacgttg tcatcttctg agaattgcgt tccagcggag ttgagaatag
     3241 catcgtggcc ctcgtcgaca tcctctgttg catcatcgtc tagggccggg gtcatgactg
     3301 cttccacgaa gtagtcgaag gtaccagagt cggtgccagg gctgaagcgc aggatgggtt
     3361 cagtaggcca ctcagggcga acatcagacc agttggtggc ggtggagtag agcgcagcca
     3421 gttcttcggt ggtcacatcg gtggcgaagt cattctcgct gctgactacg acagccaggg
     3481 catcgatccc aacctggaac tcgatcggag tgcgaggggg agtgagggct gcacaggatt
     3541 cgatttcggt gtcgcggata gcgcgggagg cgttggcgat atcggtttca ccattgacgc
     3601 agaagcgttc gatacccgca ccggacccga tggagtcgat ggtgagatta cctgcaaagc
     3661 cttcttcttc aaagcgttgc ttcatgcgtt cagcgagcgg gaagaccgtg gatgaaccag
     3721 cggcaataat gtcaccggat accgcatcag ctgcatacat cgccattgga tcttcagcgg
     3781 gtgcttcggt gacgggcgtg tcagttgctg gaacttctgt tgggggtact tcagttggtg
     3841 caggagcctc ggttgtcgcc ggcgcacatg cggcaagcag caggctcata acgataaata
     3901 caaacataaa cgaacgaaca aatttggtca tttctttttt ctccttaaaa tttatacatt
     3961 gttatcttat caatcaggtt tt
//
LOCUS       DASTJP010000166         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1185882, whole genome shotgun sequence.
ACCESSION   DASTJP010000166 DASTJP010000000
VERSION     DASTJP010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185882"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFQ23_05075"
     CDS             <1..591
                     /locus_tag="VFQ23_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9905984.1"
                     /translation="VEAGAKLQELVALEPFQEGFLGAVHDDMQAAFGNGKAAMELSGQ
                     WAPSTQKANSADQEGVGENLGLFGFPAVEGGAGAGTDVVGGGNGFAIGKNAEPEAIDF
                     VKYLTSVENQTIIAANGSGIPVVKGAETGLTDPNMLQVQQTFAAAEYFQLYYDQFLPP
                     ATGSVLNDSVQGLFAGTMTPEEVAQAVEDSVAAEIK"
     gene            666..1610
                     /locus_tag="VFQ23_05080"
     CDS             666..1610
                     /locus_tag="VFQ23_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9905985.1"
                     /translation="MQQLPAIPDILKLKRVSGAPRRLQDTLLIALFLLPALILFLLFV
                     IYPIFRSIYFSMFDWNGLGPAVDFVGLENFKTILTDKVFLKALGNVGLIVAFSLGLQL
                     PLALALAVMVGRDLPGRGIFRTIFFMPYVLAEVNAAIMWMLLFNPDPDRGLLNAIAVA
                     LSGEPIGWLSNPGLVLFSIFIALTWKYFGFHMLLYLAGLQNIPQELEEAARIDGANSF
                     QNFFFITLPLLGSTIRTSVYLSVLGSIQQFILVWIMTKGGPINASETLATYMYRFGFI
                     RFQLGYGSAVAIYMFILCLIFSLLYQRLTRQPDYLTGL"
     gene            1623..>2313
                     /locus_tag="VFQ23_05085"
     CDS             1623..>2313
                     /locus_tag="VFQ23_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9905986.1"
                     /translation="MEAYHPASRSHRLQKFLTRFFQYLILVIVVVIIFVPLVILIFGS
                     LKTTGEMYSHPYAIPNPPHWENLVAILTQPTFWRLLRNSVVLMLATTAGVVFVCGLAA
                     FVFARLEFRSKGLFFNLFTVGLMFPINVAILPVYFVLRQMNEMGIPLIDSLVGVILVQ
                     IAFNISGNIMILRGFFTAIPSELQDAAYIDGCNNFDFFWRILLPLARPALGAVAALTM
                     IVSWNDLLVPLV"
BASE COUNT          458 a          622 c          596 g          637 t
ORIGIN      
        1 gtcgaggctg gcgcaaagct ccaggaattg gttgctcttg agcccttcca ggaaggcttc
       61 ctgggcgcgg tgcatgatga catgcaggca gccttcggca atggcaaggc tgctatggag
      121 ctttccggac agtgggctcc ctccacacag aaggccaaca gcgccgatca agagggcgtt
      181 ggtgagaacc ttggcttgtt tggcttcccg gctgtcgagg gtggtgctgg cgctggaacg
      241 gacgttgtcg gtggcggtaa cggctttgca attggcaaga acgccgaacc ggaagccatc
      301 gactttgtca agtacctgac cagcgtggag aaccagacga tcatcgccgc aaacggatcg
      361 ggcatcccgg tcgtaaaagg cgctgagaca ggcttgacgg accccaacat gctccaggtc
      421 cagcagacct ttgctgctgc ggagtacttc caactgtatt acgatcagtt cctgcccccg
      481 gctacgggtt ctgtgcttaa cgactccgtg cagggccttt ttgccggcac aatgacgcct
      541 gaagaggtgg cacaggcagt tgaagattcg gttgcagcag aaataaagta accttgcaac
      601 tttgacagac ctgacaggtc tcattcaaaa gacctgtcag gtgttgatca caaaggagtc
      661 gccacatgca acaacttcct gccattccag acatcctgaa attaaagcgt gtatcggggg
      721 cacctcgtcg tcttcaagac acattgctga ttgccctgtt cctgctgcct gctcttattc
      781 tcttcctgct ttttgtcatc tatcccattt ttcgctcgat ctatttcagc atgttcgatt
      841 ggaatggctt gggacctgcg gtggatttcg tagggctgga aaatttcaaa accatcctca
      901 ccgataaagt gttcctaaaa gcgctcggga atgtcggcct gattgtggct ttctcgctgg
      961 ggttacagct gcctttggct ctggcactgg cggtcatggt gggacgcgac ctgccagggc
     1021 gtggcatttt ccgcaccatt tttttcatgc cttatgttct cgctgaagtc aatgccgcga
     1081 tcatgtggat gctgctcttt aaccccgacc cggaccgcgg ccttctcaac gcgatcgcgg
     1141 ttgccttgag cggcgagcct ataggctggc ttagcaatcc gggccttgtt ttattctcaa
     1201 tcttcatcgc tctgacatgg aaatacttcg gctttcacat gttgctatat ttagccgggc
     1261 tccaaaacat tccgcaggag cttgaagaag ctgcccgcat cgatggcgcg aatagctttc
     1321 agaatttctt ctttattacc ctgcccctcc tgggtagtac gatccgtacg tccgtttatt
     1381 tatctgtgct tggctcgatc cagcaattca ttctggtctg gatcatgacc aagggaggac
     1441 cgatcaacgc gagcgagacc ctggcgacgt acatgtatcg cttcggcttt atacgcttcc
     1501 aactgggtta tggttctgcg gttgcgatct atatgttcat cctgtgcctg atcttttctc
     1561 tgctctacca gcgcctcacg cgccagcccg attacctgac cggactctag aaaggtaaat
     1621 gtatggaagc atatcatccg gcctcacgct cacacaggtt acaaaagttc ttaaccaggt
     1681 tttttcagta tttaattctt gtcatcgtcg tcgtgattat cttcgtgccg ctcgtaatcc
     1741 tgatctttgg cagtctcaag acgaccggcg aaatgtattc gcacccttat gcaatcccta
     1801 accctcctca ttgggaaaac cttgtagcga tattgaccca gcccacgttc tggcgcttgt
     1861 tacggaacag tgtggttctt atgttggcaa ccactgccgg cgtagtcttc gtttgcggtc
     1921 tggctgcctt tgtttttgca cgactggaat tccgaagcaa aggtttgttt ttcaacctgt
     1981 ttaccgtggg tctcatgttt cccatcaatg ttgcgatttt gcctgtctat ttcgttttgc
     2041 gccaaatgaa tgagatgggt ataccgttga tcgacagcct cgtcggagtg atcctggtgc
     2101 agatcgcgtt caatatatct ggcaacatca tgattctgcg cggtttcttt accgccatcc
     2161 cctcggaatt gcaggacgcg gcctacatcg atggctgcaa caatttcgat ttcttctggc
     2221 gcatcctgct ccccttggcg cgccccgcac tgggggccgt cgcggcgctg acgatgattg
     2281 tgagttggaa tgatttgctt gtgccgctcg tag
//
LOCUS       DASTJP010000167         6598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_974518, whole genome shotgun sequence.
ACCESSION   DASTJP010000167 DASTJP010000000
VERSION     DASTJP010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974518"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFQ23_05090"
     CDS             <1..541
                     /locus_tag="VFQ23_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905987.1"
                     /translation="GKSIDEMAPVCTIMVGRTDDWMKVVASRDGIEIDPSYLDWAGIA
                     CMKKAYELYQQRGYRARLLAAAYRHLGHWAEFIGGELVVSLPYEWQLKANASDIEVKE
                     RMSIPVESKIVDEMYKEIPDFRRAYDEDGMSIEEFDEYGATVRTLRTFITSAHDLMGE
                     VREFMLPNPDIKNVESLEA"
     gene            575..2437
                     /gene="iolD"
                     /locus_tag="VFQ23_05095"
     CDS             575..2437
                     /gene="iolD"
                     /locus_tag="VFQ23_05095"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HET9905988.1"
                     /translation="MATVRLTTAQAIIKFLKNQYVHRDGKETPFFAGMLGIFGHGNVA
                     GIGQALQENPDFPYILVRNEQSGVHLACGYTKMKNRLQTFACTSSIGPGATNMITGAA
                     LATINRLPVLLLPGDIFARRNVAPVLQQLESPSSQDISVNDAFKPVSRYWDRIYRPEQ
                     LLTSLPEVMRVLTSPADTGAVTLALPQDVQAEAYDFPEELFAHRTWHISRWTSDRFLF
                     SQAVEAIRNSKRPMIIAGGGVLYSEATDALASLASQTGIPVGETQAGKSSLPYNHPQN
                     LGAVGATGTLAANRVARDADMIIGIGTRYSDFTTASKTAFQNPEVRFININVAELDSY
                     KHSAIPLNADARLTLEELTGSLKEYKVDSEYQSQIATLKEKWEQEVDRLFHLDNQPLP
                     AQSAVIGAIWESAEPKDVLVSAAGSHPGDLHKLWRAHQPNTYHLEYGYSCMGYEIPGA
                     MGVKMADPAREIYAFVGDGTYLMMPSEIVTAVQEGIKLIIVLVNNHGFASIGGLSQSL
                     GSNGFGTRYRARDSQSGQLDGKHLPVDFAANARSLGANTISTSTMEEFKQALQDARKA
                     TKTTVVVVETEREVRVPGYDSWWDVAVAETSSIDSVKKARAEYEESLKKERYFL"
     gene            2450..3370
                     /locus_tag="VFQ23_05100"
     CDS             2450..3370
                     /locus_tag="VFQ23_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET9905989.1"
                     /translation="MIKVANAPCSWGVLEFELEGEAAEYTQVLNEIVETGYQGTELGD
                     WGFMPTDPKELRREIHSRGLTLLGSFVPVMLKEPNAHATGMDAALRSARLLAEVEGNR
                     PFIILADDNGAVPERIQNAGRVLPEHGLNESEWKIFAEGAEKIAQAVKRESGLRTVFH
                     HHCAGYVEHPTEIDTLMKLTDPDLLGLCFDTGHFRFSGGDPVTGLDTHKDRIWHVHFK
                     DCSPEIAERSRAEKWDYFKALRNGIFCELGKGDIDFSRIKSDLKKIGYDGWIVVEQDV
                     LPGLGKPKESAQRNRDYLSNIGLSPSLLDS"
     gene            3392..4435
                     /gene="iolG"
                     /locus_tag="VFQ23_05105"
     CDS             3392..4435
                     /gene="iolG"
                     /locus_tag="VFQ23_05105"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HET9905990.1"
                     /translation="MTQNNDLRFGVIGAGRIGKIHAENLATRIPDVVVAAISDVNVKA
                     AQELATRLHVSAAEEDYHALLSDSTINAVAICSSTDTHAKIIIEAAEAGKHIFCEKPI
                     DLRLEKIDAALDAVEKAGVKLQIGFNRRFDPNFRKVRTMVAEGKIGELQILRITSRDP
                     APPPLAYIKVSGGIFLDMTIHDFDMARYLSGSEVEEVYTAAGVMVDPAIGEAGDLDTS
                     IITLRFANGAIGTIDNSRQAVYGYDQRVEVFGSNGMAQAHNNTPDNDVYFNADGVHSA
                     KPLYFFLERYTESFIAEMKEFVKSIRENTAPPVTGIDGRIPVVMGMAAKKSYLEHRPV
                     RLDEITTPVLQSD"
     gene            4509..5693
                     /locus_tag="VFQ23_05110"
     CDS             4509..5693
                     /locus_tag="VFQ23_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9905991.1"
                     /translation="MKDIHIGLIGTGGMGKAHATAFKNVPLVFGNEPGRPVLEIVADI
                     DAKAVEKWAGEFGFNRWTTNWQEIVQDPRVDIVDITTPNHLHAEMAIAAAEAGKHIYC
                     EKPLATTSSDAARIVAAVEKSGVISIVGFNYLKNPAQAFAKQLIESGELGEITLFRGT
                     FDQDFLANPDIPFSWRLDRALAGTGTLGDLASHTIAFAHFLVGEVVEVCGVNTTRIKE
                     RVVPASGSGYAATAQSDAEKRAVENEDIVEFLVRFDNGAIGTIGSSRIGMGRKLGLGY
                     EIQGTKGSLFYTQERMNEIKLYRHTDPDREKGFKTVYIGPEHPGYKAFFGLAGIGLGY
                     NDQKIIEAHDLISAVALHQPVQPDVRFAYEVNKVIDAADLSCEEHRWVQVSEFDTAVD
                     RA"
     gene            5705..>6598
                     /locus_tag="VFQ23_05115"
     CDS             5705..>6598
                     /locus_tag="VFQ23_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET9905992.1"
                     /translation="MRIGLVTDSLGHLSFDDLLETAATLGIQTLEFGCGGWSSAPHLN
                     LNLLLENETERDNFIAKIRGHGLEISALNCSGNQLAPGQRGKSNDQVVRNTIKLAHLL
                     GIRRIVIMSGLPGGPGDQNANWITTAWPPEGHEILRYQWDDVAIPYWRDLANFARENG
                     VEKICVEQHAHQLVYNTETLLRLRDAVGEIVGVNFDPSHALWMGGDPLRAIRQLRGAI
                     YHVHAKDTRIDPHNSEVNTLLETKPNERVAERSWNYVTLGYGHGETWWRDFVIQLKQN
                     GYDDVLSIEHEDYSLPPLVGVQ"
BASE COUNT         1647 a         1593 c         1841 g         1517 t
ORIGIN      
        1 gggcaagtct attgatgaga tggcgcctgt ctgcaccatc atggtggggc gtaccgacga
       61 ttggatgaag gtcgttgcaa gtcgtgatgg aattgagatc gatccgtcct atctggattg
      121 ggctggcatt gcctgcatga aaaaagccta tgaactttat caacagcgcg gttaccgggc
      181 tcgtctgctt gccgcagcgt atcgacatct gggacactgg gcggagttca tcggcggcga
      241 gttggtcgtc tcgttgccct atgagtggca gctcaaagcc aatgccagcg atattgaagt
      301 caaagagcgg atgagcattc cggtcgagtc aaaaatcgtg gatgagatgt ataaagaaat
      361 tcccgatttt cgccgcgcct atgacgaaga tggtatgagc atagaggagt tcgacgagta
      421 cggcgcgacc gtacggacct tgcgcacctt catcacgtcc gcccatgacc tgatggggga
      481 agtccgtgaa tttatgttgc ccaaccccga tatcaagaac gttgaaagtt tagaggcgta
      541 gcaagccttt cactttcata gtgaggagaa atccatggca acggtacgat tgacgactgc
      601 acaagccatc atcaaatttc tgaagaatca gtatgtccat cgcgacggca aggaaacgcc
      661 tttctttgcg gggatgctgg gcatctttgg tcatggcaac gtggcaggga tcggacaggc
      721 cctgcaggag aacccggact ttccttatat cctggttcgc aatgaacaat caggtgtgca
      781 tctggcttgt ggctacacca agatgaagaa tcgcctgcag acctttgcct gtacctcctc
      841 gattggcccc ggtgcgacga atatgatcac cggtgcagcg ttagcgacca tcaatcgatt
      901 gcctgtgctt ttgctcccgg gcgatatctt tgcacggcgt aacgtcgccc ctgtgcttca
      961 acaacttgag tcaccttcct cgcaagacat cagcgtcaac gatgcgttca aacccgtcag
     1021 ccgttattgg gatcgtatct atcgccctga gcagctcctc acatctttac cggaagtaat
     1081 gcgggtactc acttctccgg cggatacggg agcagtcaca ctcgctttgc cacaggatgt
     1141 ccaggcggag gcctatgatt tccctgaaga actctttgct catcggacct ggcacatctc
     1201 gcgctggacc tcggaccggt tcctgttttc tcaggcagtg gaagccatcc gcaacagcaa
     1261 acgaccgatg atcatcgcgg gtggtggcgt gctttacagc gaagcgacgg atgcactggc
     1321 gagcctcgcc agtcagacgg gaattccggt tggcgaaacg caggcgggaa aaagttctct
     1381 tccgtataac catccacaga atctgggtgc tgtgggcgcg acgggcacgt tggctgctaa
     1441 tcgtgttgca cgtgatgctg atatgatcat tggcattggc acacgctact ccgatttcac
     1501 cacggcttcc aagaccgcgt tccaaaaccc tgaggtgcgc tttattaata tcaacgttgc
     1561 cgaactcgat tcatacaaac attctgctat tcccctcaat gcagacgcgc gcctaacgct
     1621 ggaagaattg actgggtcgt tgaaagaata caaagtagat tccgaatatc aatcgcaaat
     1681 cgctacgctt aaggaaaaat gggagcagga ggttgatcgg ttattccatc ttgataacca
     1741 accattgcca gcgcaaagtg cagtgatcgg cgccatctgg gaatctgcag aaccaaagga
     1801 tgtgcttgtc tccgcggcag gcagccaccc cggcgacctg cataaattat ggcgggcaca
     1861 tcaacccaat acctatcacc tcgaatatgg ctattcctgc atgggttacg aaattcccgg
     1921 tgcgatgggt gtcaaaatgg cggacccagc gcgcgagatc tacgcgtttg tgggcgatgg
     1981 aacctatctg atgatgccat cagagatcgt gactgccgtg caggagggca tcaagctgat
     2041 tatcgtcttg gtcaacaatc acggctttgc cagcatcggt ggtctcagtc aatcgctagg
     2101 cagcaatggg tttggtaccc gatatcgagc acgagactca cagagcgggc agttggatgg
     2161 taaacatttg cctgtggatt ttgccgcgaa tgcccgcagc ctgggcgcga atacgatcag
     2221 cacttccacg atggaagaat tcaaacaagc actgcaggat gcccgtaaag ctacgaagac
     2281 tacggttgtt gtggttgaaa cggagcgtga ggtgcgcgtg ccagggtatg actcgtggtg
     2341 ggacgtggcg gtagcggaga cctcttcgat cgatagcgtg aagaaggcca gggcggaata
     2401 tgaagagtca ctaaagaagg agagatattt tctataaaaa ggagttacga tgattaaagt
     2461 cgcgaatgcg ccctgttcat ggggagtgct cgagtttgaa ctcgagggcg aggcggccga
     2521 atacacccag gttttgaatg agatcgtcga gacgggctac cagggtaccg agcttggcga
     2581 ttggggtttt atgccgacag acccaaagga attgcgacgc gaaatccatt cccgtggact
     2641 gactctgctt ggctcgtttg tgccggttat gctcaaagaa cccaacgctc atgcaactgg
     2701 catggatgcg gctctgcgca gcgcgcgtct attggctgaa gtggaaggca accggccctt
     2761 catcatcctt gcggatgaca atggagcggt tccggaacgt atacaaaacg ccggccgcgt
     2821 tcttcctgaa catggattaa atgagagcga atggaaaata tttgccgaag gcgcagaaaa
     2881 aattgcgcag gcggtaaaaa gggaatcagg tttacggact gtgttccacc atcactgcgc
     2941 ggggtacgtg gagcacccaa ccgagatcga tactctcatg aagttgacag acccagactt
     3001 gctggggtta tgctttgata caggtcactt tcgattcagc ggcggggacc cagtcacggg
     3061 tcttgatact cataaagaca ggatctggca tgttcacttt aaagattgca gccctgaaat
     3121 tgctgaacgg tcacgcgcag aaaagtggga ttactttaag gcgcttcgga atgggatctt
     3181 ctgtgaacta ggaaaaggtg atattgactt ttcacggata aagtctgacc tgaaaaaaat
     3241 tggttatgat ggctggatcg ttgtcgaaca ggatgttctt cccggcctgg ggaaaccaaa
     3301 ggaatcggcg cagcgcaatc gtgattattt gtccaacatt ggtttgagcc ccagcctgtt
     3361 ggatagttaa tgcttgagga gagaaaaaag tatgacacaa aataatgatc tccgctttgg
     3421 tgtgattggc gccggacgta tcggcaagat ccacgcggaa aatttagcca cgcgtattcc
     3481 agatgtggta gttgctgcaa tttcagatgt caatgtgaaa gccgcgcagg agttggccac
     3541 acgcctgcac gtctccgctg cagaagagga ctaccatgcg ctcctctccg attcaaccat
     3601 aaacgccgta gcgatttgct cgtccacaga tacgcatgca aaaatcatca tcgaggcggc
     3661 tgaagctggg aagcatattt tttgcgagaa accgattgat ctccgtctgg aaaagatcga
     3721 tgccgcgctg gatgccgtgg agaaggccgg tgtaaaactg caaattggtt tcaatcgccg
     3781 cttcgacccg aacttccgca aagtccgtac catggttgcc gaaggaaaaa tcggtgaatt
     3841 gcagatcttg cgcatcacca gccgcgaccc ggctcctccg cctcttgctt acatcaaagt
     3901 ttccggtgga atcttcctgg atatgaccat ccatgacttt gacatggcgc gttatctgag
     3961 cggaagtgaa gtggaagaag tctacaccgc cgcgggcgtg atggtggacc ctgccatcgg
     4021 cgaggctggc gatctggata cttcgatcat tacgcttcgt tttgcaaatg gcgcgatcgg
     4081 cacgatcgac aacagccgac aggctgttta cggatacgac caacgagtgg aagttttcgg
     4141 ttcgaacggc atggctcagg cacacaataa cacgccggac aatgatgtgt atttcaatgc
     4201 ggatggcgtc cattcggcca agccgctcta cttcttcctt gaacgttata ccgagtcatt
     4261 cattgcagag atgaaggagt ttgttaagag cattcgggaa aatacagctc ctccggtgac
     4321 tggcatcgat ggtcgcattc cggtggtgat ggggatggca gcgaaaaaat cgtacctgga
     4381 gcatcgaccc gtgagattag atgaaatcac gactccagtc cttcaatcag actgaaattt
     4441 ctttcccaca gcagttgatc tacgtacgac ccaaataaat cttgcctgcg aataaaggag
     4501 ttgtatcgtt gaaagacata catattggat tgatcggaac cggagggatg gggaaagccc
     4561 acgccacagc atttaagaat gttcccctgg ttttcggaaa tgagcccggc agacccgttc
     4621 tggaaattgt cgcggatatt gatgcgaaag ccgtggaaaa atgggctgga gaatttgggt
     4681 ttaatcgctg gacgaccaat tggcaggaga ttgtccagga tcctcgtgtg gatattgtgg
     4741 atattacgac tcccaatcat ctgcatgcag agatggcaat cgccgccgcc gaggcaggga
     4801 agcacattta ttgtgaaaaa cctctggcaa ctacttcgtc ggacgcggcc cgtattgtgg
     4861 cggccgtaga aaaatccgga gtcataagca tcgtaggctt caattacctg aaaaaccccg
     4921 cccaggcatt tgccaaacaa ctgattgaat ccggggaatt aggcgagatc accttgtttc
     4981 gaggaacctt tgatcaggat ttccttgcca accccgatat cccctttagc tggcgattgg
     5041 atcgcgcctt agcagggaca ggtaccttag gggatcttgc ttcacacacg attgccttcg
     5101 cccatttttt ggtgggtgag gttgtggagg tgtgtggcgt aaatacgacg agaatcaaag
     5161 agagagttgt gccagccagt ggctcaggct atgccgcgac cgcgcaaagt gatgccgaga
     5221 agcgagcggt cgaaaatgaa gatatcgtgg agttcctcgt ccgttttgac aatggggcaa
     5281 ttggaaccat tggtagcagt cgaatcggga tgggacgcaa gctggggtta ggctatgaga
     5341 ttcaaggcac aaagggcagt ctgttttaca cacaggaacg catgaatgaa atcaagctgt
     5401 atcgccatac agaccccgac cgggaaaagg gctttaaaac ggtttacatc ggacccgaac
     5461 acccggggta taaagccttc ttcggtttgg cagggatagg cctgggatac aacgaccaga
     5521 agattatcga ggcgcatgat ctcatctcag ccgtggcctt gcaccagcca gttcagccgg
     5581 atgtccgttt tgcttatgaa gtaaacaagg taattgatgc agccgacctt tcctgtgaag
     5641 agcatcgctg ggttcaggtg agtgagtttg ataccgcggt ggatcgagca taaaggacaa
     5701 ctgcatgcga atcggattag tcacagatag cctggggcat ttgtcgtttg acgacctgct
     5761 ggaaacagcc gccacccttg gaattcaaac cctggaattt ggttgcggtg gctggtcatc
     5821 ggcgccgcat ctgaatctga atctcctttt ggaaaatgaa acagaaagag ataacttcat
     5881 cgcaaagatt cgcggccacg gcctggagat cagcgcgctg aattgctcgg gcaatcaact
     5941 tgcccccggg caacgtggga aatcgaacga tcaggttgtg cgcaatacaa tcaagctggc
     6001 tcacttactt ggcatccggc ggatcgtgat aatgtctggg ttgcccggag gtccaggtga
     6061 ccagaatgca aattggatca caacggcctg gcctcccgaa gggcatgaga tactgcgcta
     6121 tcaatgggat gacgtagcga ttccctattg gcgcgatctg gcaaattttg cacgcgaaaa
     6181 tggtgtggaa aagatttgcg tggaacagca cgcccatcag cttgtgtaca acacggaaac
     6241 tttgctgaga ctacgcgacg ccgtcggtga aattgtggga gttaattttg atcccagcca
     6301 tgctctctgg atgggtggtg atcctctgcg cgcgattcga caattgaggg gcgccatcta
     6361 tcacgttcat gccaaggaca cgcgcatcga tccgcataac agcgaagtca acacattgct
     6421 tgaaaccaaa cccaatgagc gtgtggctga gcgttcctgg aactatgtga ccctgggcta
     6481 tggacacggt gaaacctggt ggcgtgattt tgtgatccag ttaaagcaaa atggctacga
     6541 cgatgtgctg agcatagagc atgaagatta cagtctgccg cccctcgttg gcgtacag
//
LOCUS       DASTJP010000168         3244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1443393, whole genome shotgun sequence.
ACCESSION   DASTJP010000168 DASTJP010000000
VERSION     DASTJP010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443393"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFQ23_05120"
     CDS             complement(<1..387)
                     /locus_tag="VFQ23_05120"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9905993.1"
                     /translation="MNTYLSFLQLRPKFRSLWLAQVISLSGDWFNTIASVIIINRYAP
                     SGLAVGSLFIARALPPFLLGPLAGVVADRFDRRKILILSDLLRAGIVLGFLLVDRPER
                     LWLLYVLTVLQFSVSTFFEPARAALVP"
     gene            756..884
                     /locus_tag="VFQ23_05125"
     CDS             756..884
                     /locus_tag="VFQ23_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905994.1"
                     /translation="MTTLLNSGEFLHTPSEDTFVMQGITWKFPIYETIDEFFRGLR"
     gene            complement(973..1593)
                     /locus_tag="VFQ23_05130"
     CDS             complement(973..1593)
                     /locus_tag="VFQ23_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905995.1"
                     /translation="MKIFSIPALFLTIALLISACGGGGPPATPTISAVEVQNTVIVAA
                     MTVVAETQAAIPTATPPPPTATFTFTPPATNTLPPLPTLDATFTSAPSSNSGADDPCI
                     NQALPAELQGETVRLRIHNSTRVTLALSVYLNQTTPQVVCGYRTYTIASEETLAINNL
                     VEGCFTLWAWNPDQDNYFIVTNGASSCITRFDLAVFDVTTRGISRR"
     gene            complement(1645..1989)
                     /locus_tag="VFQ23_05135"
     CDS             complement(1645..1989)
                     /locus_tag="VFQ23_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9905996.1"
                     /translation="MLEIGAIVWGVRDLHRSIRFWSEALNYKLSREADVDFAILIPKE
                     GAGIQLSLNAAVKSEKPRRHHIDLFTTDQAKEVERLLSLGATRVDWNYDPHADYVVLA
                     DPDGNTFCVVQS"
     gene            complement(2234..2497)
                     /locus_tag="VFQ23_05140"
     CDS             complement(2234..2497)
                     /locus_tag="VFQ23_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9905997.1"
                     /translation="MTDMHLMNDATTHMDASPHEASDVQGIGCLGIVSGITFLLLVLF
                     MAFGYPHYNTSRDWVFWVVGFGTLLIEAIVIYTAIFISRVVNT"
     gene            complement(2921..>3244)
                     /locus_tag="VFQ23_05145"
     CDS             complement(2921..>3244)
                     /locus_tag="VFQ23_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9905998.1"
                     /translation="EGSSILGTLGGIRLEPFGFFHNIGDLEINSTADVDQIPYRWQNV
                     HLGGDVYDSSEHHWVAALQGRVDLLPSAELALNTMLISEGIYLSEQLGREVTAEEVRE
                     TSRAQ"
BASE COUNT          868 a          778 c          798 g          800 t
ORIGIN      
        1 tgggacgagc gcagcacgtg ccggttcaaa aaaagtcgag acgctgaatt gtagcaccgt
       61 caacacatac aggagccaca gccgttccgg gcgatccacc agcaaaaagc caaggacgat
      121 gcccgcccgc aagagatcgc tcagaatcag gattttgcga cggtcgaatc gatctgcgac
      181 aacgccggct aagggtccca gcaagaatgg aggcaaggcc cgggcgataa acaagcttcc
      241 cactgccagc ccggagggcg cataacgatt gatgatgatc acggaggcaa tggtgttgaa
      301 ccagtcaccg ctgaggctga taacctgtgc cagccataag gatcgaaatt tgggtcgaag
      361 ttggaggaaa gagagataag tgttcacgcg aacaattata tctgccgaat gttttgggtt
      421 tacaaaccgc atgccgtttg aagacgtata ataatcacgt ccatgttcgg cataacagtc
      481 ctaaaggatg catgatccat gaactctacc cgcgaggaaa aactatcgcg atctgctctg
      541 gttgattttc tgtggcagtc ggaaaatctg accgatggag tgtatgttgc atcgacagac
      601 gccagttggg acatgatttt cacacgaacc agggccggtc agccaaaagt cttgctgtgt
      661 ggtccttcct ataaaacagc gcaagtccct tttgttcggg ggagaaacat attggtattt
      721 gtttcaaacc ctgggcagtg tttaccggta ttccaatgac aaccttgctc aatagcggag
      781 aatttctaca cacaccttcg gaagatacgt ttgtgatgca gggcataacc tggaaattcc
      841 cgatctacga gaccatcgat gaatttttcc gcgggcttcg ttagttatca aagtggcgta
      901 tgggatgtgc cgccatataa tgcaagtcac cccgtttgtg gggtgacttt tgcctggttt
      961 gcgaatgaac acctatcttc ggctgattcc ccttgtcgta acatcaaaga cggccagatc
     1021 aaaacgagtg atgcagctac tggcaccatt tgtcacaatg aaatagttgt cctgatcggg
     1081 gttccatgcc caaagggtga aacatccttc taccaggtta ttaatggcaa gcgtttcttc
     1141 ggaggcgatc gtataggtgc gatagccgca gacaacttgc ggcgtagttt gattgaggta
     1201 caccgaaagt gcgagtgtca cccgggttga gttgtggatt cggagtctga cggtctcgcc
     1261 ctgcagttct gccggtaaag cctgatttat acagggatca tccgcaccgg agttgctact
     1321 tggggcagac gtaaatgtgg catctaaagt cggtaaggga ggcagcgtat tggtcgccgg
     1381 gggcgtaaag gtaaatgttg ctgttggtgg aggtggagta gcagtcggga tggccgcctg
     1441 ggtctctgcg actacagtca tagctgccac aatgacggtg ttttgtacct caacggcgct
     1501 gatagtgggc gtcgccggtg ggccacctcc gccacaagcg ctgatgagca atgctatggt
     1561 gagaaacaaa gcaggaattg aaaaaatttt catacggata tctccttttt ggtagcaatc
     1621 cccatcttat cttcaagtgc atctttatga ctgaacaaca caaaaggtat ttccatccgg
     1681 gtcggccaga acaacataat cggcatgagg atcataattc caatctactc gagttgcgcc
     1741 gaggctcagc aaccgctcca cctctttcgc ttgatcggtt gtgaacaagt cgatgtggtg
     1801 acgcctgggt ttctctgact taacggctgc attcagcgat agttgtattc ctgctccttc
     1861 tttcggaata agaatagcaa agtccacatc agcttcccgg ctcaatttgt aattcagcgc
     1921 ctcgctccaa aaccgtatgc ttcgatgaag gtcccggact ccccatacga tcgctccgat
     1981 ttccaacatg atccatcctc caaaatcaag ttccattatt ttgagcagag atcgcccaaa
     2041 taatttctcc ggaatgatta tagcatttct gaaagcttgc actttcttaa cgacgggata
     2101 tgagaaccat ttccagactt ctaaagtgcc tgcgcttcgt agggaatttt aaaacgaaaa
     2161 cttgctggca ggcgatataa ccagcaagtt caaagtattg aatagtctac gcaaaatatg
     2221 ggtcggtaaa cgtctacgta ttgacgactc gagaaataaa tattgccgta tagatgacaa
     2281 tggcttcgat aagcagggtg ccaaagccca cgacccaaaa tacccagtcg cgagacgtat
     2341 tgtaatgagg atagccaaaa gccataaaca agacaagtaa caggaaggtt atcccagata
     2401 caatgcccag gcatccgatt ccctgcacgt ctgaagcttc atgtggagag gcatccatat
     2461 gggtggtggc gtcattcata agatgcatgt cggtcatagc ttaactacct cttagtggtt
     2521 ggagcagatt caaattccgt cacctgccag atacggctaa ggcaccgaaa tcatcgtgtc
     2581 gcttcgagtt gagacagtct gatcgcggtc catcgaaatc atattcgaag cattttagta
     2641 gttttacaaa tttttttata tacaggaaat ccctacctga aatcaattct cccttaaccg
     2701 attcttgggc gaagactccc aaatcgcagt aagggctcgg catcggatga atttgtcatg
     2761 gcgttccacc atcgaagtaa aaaccaacaa aataaagtgt cgcggatacc tcgtgtatcc
     2821 gcgacactta ttggatgatt tcttacactc aagaaccaag actctctgtg agagtgatat
     2881 ctcttagatc tatgaggaga tcttttttac agacaaatat ttactgggca cgcgacgttt
     2941 cgcgcacttc ctcggcggtc acttcacgac ccagttgctc cgagagatag atgccttcag
     3001 agatgagcat ggtgtttagg gccagttcag cactcggcag aaggtccacc cgtccctgta
     3061 acgcagccac ccagtgatgc tcggaactat catagacgtc gccacccaaa tgcacattct
     3121 gccaacgata tggaatttga tcaacgtccg ccgtactgtt gatttcaaga tcgccaatgt
     3181 tatgaaagaa accaaacggt tcgagacgaa tgccacccag cgttccgagg atgcttgagc
     3241 cttc
//
LOCUS       DASTJP010000169         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_465739, whole genome shotgun sequence.
ACCESSION   DASTJP010000169 DASTJP010000000
VERSION     DASTJP010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465739"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..2462)
                     /locus_tag="VFQ23_05150"
     CDS             complement(87..2462)
                     /locus_tag="VFQ23_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9905999.1"
                     /translation="MKYQIHKAVVIGSGTMGAAIAAHLANSGVPVTLLDIPAKDADDK
                     NKIVKDGWDRCLKAKPANLMSAELKTFVKLGNLEDDFDTVAEADWICEAIVENLKIKQ
                     QLMARIDAVRKPGAVVSTNTSGIPITSIAEGRSKEFKKLFLGTHFFNPPRYLKLLEVI
                     PTKDTDKDVVEFMSWFGEYRLGKGIVVCKDTPNFIGNRVFFGMSTFAMNFILENNYTV
                     EEVDSLTGPLIGRPKTAMFRLADLVGLDVWEHVGRNLGPAIPHDELGQQYLAAEKPKQ
                     LMSSMLERKWLGNKTRIGFYKEVRGEDGKREFWSLDLQTLEHVPPTKPRFDSVKAAKD
                     VESLEGRLKVMLEAPSGDKAAKLIRALTYQSFQYASSIIPEVADAPKPIDDAIRWGFS
                     HEAGPFEIWDRLGVKDIVAKMQAEGYAPAKWVNDMLKAGVESFYEYKNGEIVGVYDVV
                     KKKYVKIKRPKAVVMLKRQKVISQNMGATLRDMGDGVACVEFHTKMNSLDDDIMNIAN
                     EAFGRLDKDFDGLVIGNEGENFSAGANVFMMVVGAQQGMWDMLDGAVRKLQDMNMRMR
                     YSPKPIVVAPAGLTLGGGCEITMHASRIVAAAESYIGLVELGVGLIPAGAGTKEMVRR
                     IVNPVMRIENAEPLAALQKVFLQIGQAKVATSAEEARSMGILSPQDRVVMNRSHLLSE
                     AKNEVLHMIAAGYKPPAPEPIYAAGRDALAALRIGAWMFKEGQYITQYDHHVAGRLAY
                     VMCGGELTRPQWVSEQYILDLEREAILSLFGEEKTQARMWNILQTGKPLRN"
BASE COUNT          570 a          731 c          555 g          644 t
ORIGIN      
        1 ggcgtgcgga ctgcagatac aataacggct tctttcatca aaaaaactcc tttgcatcaa
       61 cgtttaacgg ttcaaacgag acttcgttaa ttcctcaggg gtttgcccgt ttgcaaaatg
      121 ttccacatgc gggcctgggt cttttcttca ccgaacaacg agaggatcgc ttcacgctcc
      181 agatccagaa tgtactgctc actcacccac tgtgggcggg tgagttcgcc gccacacatc
      241 acgtaggcca gccttcctgc aacgtgatgg tcgtattgtg taatgtactg accttccttg
      301 aacatccacg cgccaatccg gagcgccgcc agcgcgtcac ggcccgcggc ataaattggt
      361 tctggcgcag gtggtttata gcccgccgcg atcatatgca gaacctcgtt ctttgcttcg
      421 gaaagtaaat gtgaacgatt catcaccaca cggtcttgcg gactcaagat acccatgctg
      481 cgggcttctt ctgcactggt cgcaaccttt gcctgcccga tttgtaaaaa tactttctgt
      541 agcgctgcaa gcggctcggc attttcgatg cgcatgacgg gattcacgat gcgacgcacc
      601 atctcctttg tacccgcgcc agcagggatc agacccacgc caagttcgac aagcccgatg
      661 tagctttcgg cggcagccac aattcgcgag gcgtgcattg ttatttcaca gcctccgccg
      721 agagtaagac cggctggtgc aaccacaatt ggttttggcg aatagcgcat acgcatgttc
      781 atgtcctgaa gcttgcgcac cgcgccatcc agcatatccc acatcccttg ctgggcccca
      841 acgaccatca taaagacatt tgcgccggcg ctgaaatttt cgccctcgtt gccaattacg
      901 aggccatcga aatccttatc gaggcgccca aacgcttcat tggcgatatt catgatgtca
      961 tcatcgagtg agttcatttt agtatgaaac tccacacacg ctacaccatc gcccatgtcg
     1021 cgcagtgtcg cgcccatatt ttgtgagatt accttctgcc tctttaacat caccaccgcc
     1081 ttgggcctct taatcttcac gtattttttt ttgaccacat catagacgcc aacgatctct
     1141 ccgtttttgt actcataaaa cgactccacc ccagccttga gcatgtcatt cacccatttg
     1201 gcaggtgcat aaccctccgc ctgcatcttc gcaactatat ccttaactcc gagcctatcc
     1261 caaatttcaa atggacctgc ttcgtgtgag aagccccagc ggatcgcatc gtcaatcggt
     1321 ttgggagcat ccgcgacttc ggggataata gacgacgcgt attggaagct ttgatacgtc
     1381 agcgcgcgaa tcaatttcgc tgccttgtcg cccgaagggg cctccagcat gacctttagc
     1441 ctgccttcca aactctcaac atccttcgcc gctttgaccg aatcaaacct cggcttggtc
     1501 ggcgggacat gttcaagagt ttgtaagtct aacgaccaga attctcgctt gccatcctcg
     1561 ccacgcactt ctttgtaaaa accgatcctg gttttatttc cgagccattt gcgctccagc
     1621 atcgagctca tcaactgttt tggtttttct gccgctaagt actgctgccc caactcatcg
     1681 tgcggaattg ccgggcccag gtttcgcccg acgtgttccc aaacgtccaa gccgaccaaa
     1741 tctgccagac ggaacatcgc cgtctttggt cgcccgatca atgggccggt gagtgaatcg
     1801 acttcctcga cggtatagtt gttttcgagg atgaagttca tcgcaaaggt actcattcca
     1861 aagaacacgc gattcccaat gaagttcgga gtatccttgc agaccacaat ccccttaccc
     1921 aagcgatact cgccaaacca ggacataaat tcgacaacgt ccttatccgt atcttttgtg
     1981 gggatgactt ccaacaactt caaatagcgc ggcggattga aaaaatgtgt tcccaggaaa
     2041 agcttcttga actctttgga acgcccttca gcaatggagg taataggaat tccggacgta
     2101 ttcgtggaaa ccaccgcccc cggcttgcga accgcgtcta tccgcgccat caactgttgc
     2161 ttgatcttca aattctcgac aatggcttca cagatccaat ctgcttctgc aactgtatca
     2221 aaatcatctt cgagattccc aagcttgacg aaggtcttca attcggcaga catcaaattg
     2281 gcaggcttgg ctttcaggca ccgatcccag ccatctttga caatcttgtt tttatcgtca
     2341 gcgtctttgg ccggtatgtc caataaggta acaggcacgc ccgaatttgc caggtgtgct
     2401 gcaatcgctg cgcccattgt ccctgagcca atgacaacag ctttgtgaat ttggtatttc
     2461 ataatgtcct actctcctgg atggtcgagt agcctggagc
//
LOCUS       DASTJP010000170         3623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_690812, whole genome shotgun sequence.
ACCESSION   DASTJP010000170 DASTJP010000000
VERSION     DASTJP010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690812"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFQ23_05155"
     CDS             complement(<1..735)
                     /locus_tag="VFQ23_05155"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HET9906000.1"
                     /translation="MNVKIISVILVLALASLACGFTIELPPQAQTGPDVEESITVADP
                     ESDETRLSLNFGAGELRLSSGAEGLVEGTALYNVDQLKPEIIKNGGNIEIKQGDLDGL
                     LPADKMKNRWDLQLGNTPMDLEIDAGAYEGNMDLGGLALKSLTVKDGAAQVNLSFSEP
                     NLIEMSHMSYSTGASEVTLSDLANANFSTFDFDSGAGSYTLDFSGELQRDASVKIDSG
                     LSELILIVPDGVNATVTIEGGLTDINT"
     gene            complement(836..2290)
                     /gene="radA"
                     /locus_tag="VFQ23_05160"
     CDS             complement(836..2290)
                     /gene="radA"
                     /locus_tag="VFQ23_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET9906001.1"
                     /translation="MPKTQTRFVCQECGRVAASYMGKCPQCGSFNSMVEEVIHDEPVN
                     KGSAVRGLTGRSAPRSIGDISSDAEDRINLPIGEFARVLGGGIVPGSIVLVGGDPGIG
                     KSTLMLQMAMEMAAHKRVLYVSGEESERQIKMRAVRLANNFGTSQNNGSSKDSPAALP
                     KNLLLVTETNLEIIFNHIQEVKPELLIVDSIQTVYLSEMDSSAGSVSQVRECSSQLRE
                     LAKTSGISVFVIGHVTKEGNIAGPRVLEHIVDTVLYLEGDRFQAYRLLRSVKNRFGAT
                     SEVGVFEMREGGLAEVTNPSEAFLAERMINAAGSAIAVTMEGTRPILVEIQGLTSPTQ
                     FGNARRTANGVDFNRLLLITAVLTRRVGLKLSEQDIFVNVVGGLQIDEPAADLAVATA
                     IASSWRDISVKADAVLIAEIGLAGELRMPGQMPGRLKEAQKLGFKTAIVPKALRKGEP
                     YPKGIEIVEVRSLDQALNAAFASASGPSKARREA"
     gene            2411..3226
                     /locus_tag="VFQ23_05165"
     CDS             2411..3226
                     /locus_tag="VFQ23_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET9906002.1"
                     /translation="MTEWFVQLGPVWQAFIATTFTWLLTALGAAMVFFFKQVNRKLLD
                     GMLGFAAGVMIAASFWSLLAPAIEMTQETSSLPVWFPAAVGFLLGGAFIRLIDFFLPH
                     LHLGFPTNEAEGIQTKWRRSILLVLAITLHNFPEGLAVGVAFGAVAADLPSATLVSAI
                     ALAIGIGIQNFPEGVAVSVPLRSEGMSRFRSFLYGQSSALIEPVAGVLGAGAVLLMRP
                     ILPYALAFAAGAMIFVVVEELIPESQLAKNTDFATSGAMLGFVIMMILDVALG"
BASE COUNT          819 a          929 c          908 g          967 t
ORIGIN      
        1 tgtattgatg tccgtcaacc caccctcgat tgtgacggtt gcattgactc catccggaac
       61 aatgagtatc aactcgctca agccgctgtc gattttgact gatgcatcgc gttgtaactc
      121 gcctgaaaag tccaatgtat aactgccggc gccgctgtca aaatcaaatg tactgaaatt
      181 cgcatttgca aggtcactca gtgtcacttc tgatgcacct gttgaatatg acatatgtga
      241 catttcgatc aggtttggct ctgagaatga aaggtttacc tgtgctgcgc catctttcac
      301 ggtcagagat ttgagtgcca gcccgccgag atccatattg ccttcgtacg cgcctgcgtc
      361 aatctccagg tccattggag tgttgccaag ctgaagatcc cacctgttct tcattttgtc
      421 agccgggagc aagccatcta aatctccctg tttgatctcg atgttgccgc catttttgat
      481 gatctcaggc tttaactggt ctacgttgta aagcgctgtc ccctccacga gcccctcggc
      541 gcctgaagat aatctcaatt cacctgcgcc aaaatttaat gagagacgcg tctcatcgga
      601 ctctgggtct gccaccgtta tggattcttc aacgtcgggt ccggtctggg cctgaggtgg
      661 cagttcaatc gtaaatccgc atgccaaact tgccaacgca agtacaagaa ttactgaaat
      721 gatttttacg ttcatcgaat atctccttct gtgccttata cggcggagat aggaattggt
      781 tgcaaaataa aaaaccctct cctatagaga gagggtagag tgagagcgat tacggttatg
      841 cttccctgcg cgctttggat ggtcctgagg cggaggcaaa agcagcattc aacgcctgat
      901 cgagagaacg tacttcgaca atctcaattc cctttgggta aggttcccct tttcgcaagg
      961 cctttggcac aatggcagtt ttaaaaccga gcttttgtgc ttccttaaga cgccctggca
     1021 tttgcccggg catacgcagt tcgcctgcca gaccaatctc agcaatcaag acggcatctg
     1081 ctttgacaga gatatctctc caggaagaag caatcgcagt tgcgactgct aaatcggccg
     1141 cgggctcgtc gatttgcaag ccgccaacca cgtttacaaa aatatcctgc tctgaaagtt
     1201 ttagtcctac tcttcgagtc aatacagctg tgattaagag caggcggttg aagtccacac
     1261 cgttggccgt tcggcgcgca ttgccgaatt gcgtgggcga ggtgagccct tgaatttcta
     1321 caaggatggg gcgtgtgcct tccatggtga ccgcgatggc ggaccctgcc gcattgatca
     1381 tacgctcggc gaggaaggcc tccgatggat tcgtcacctc tgccagcccg ccttcgcgca
     1441 tttcgaacac gcccacttca gatgtggcgc cgaaacgatt ctttaccgag cgcagtaaac
     1501 gatacgcctg gaagcgatct ccttcgagat agagcaccgt gtccacgatg tgttccagta
     1561 cccgcggacc cgcgatatta ccttcttttg tcacatgacc gatgacaaag accgagatgc
     1621 cgctggtctt ggccagttcg cgcagttgtg atgaacattc acgaacctga ctgaccgagc
     1681 cggctgatga atccatttct gagagataca cagtttggat ggagtcaacg atcagtaatt
     1741 caggtttcac ttcctgaata tgattgaaaa tgatttccag atttgtctct gtcaccagta
     1801 gcaaattttt gggcagggct gcaggcgaat ctttggagga tccattattc tgcgatgtcc
     1861 caaaattgtt tgctagtcgt acggcgcgca tcttgatttg tcgttcagac tcttctcctg
     1921 aaacatacag tacgcgtttg tgcgcggcca tttccattgc catttgcagc atcagcgtgg
     1981 atttgccgat gcccgggtcg ccacccacca ggacgatcga gccggggacg atcccacctc
     2041 ccaacacgcg cgcaaattcc ccgatgggga gatttatgcg atcttctgca tcggaagaga
     2101 tatcgccaat ggagcgcggt gcagaacggc cggtcagccc acgaactgca gagcctttgt
     2161 tcacaggttc atcgtggatg acttcctcca ccatgctgtt aaaggatccg cactgcgggc
     2221 attttcccat ataggatgcg gcgactcgtc cgcattcctg acagacaaaa cgagtttgtg
     2281 tttttggcat ttagaacctc gtaaggacag tataacagaa tttatgttct atcttccaga
     2341 atagatttga agtttgctat actatgaacg cgatctcaag cctggtagtt atcgatggga
     2401 gaaaattatc atgaccgagt ggttcgttca acttggcccg gtgtggcagg catttattgc
     2461 tacaaccttt acctggctgc taacagcatt gggcgcagcc atggttttct ttttcaagca
     2521 agtcaaccgc aaattgctgg atggtatgct gggattcgct gccggtgtaa tgattgcagc
     2581 gagtttctgg tcgttgcttg cacccgcaat tgaaatgaca caagagacga gcagtttgcc
     2641 ggtctggttt cccgcggcag ttggcttcct cctgggcggt gcattcatcc gtctaattga
     2701 cttcttcctc ccgcacctcc atttaggatt ccccaccaat gaggcagaag gtatccaaac
     2761 taagtggagg cggagtattt tgcttgtgct ggcgatcacg ctgcacaatt tcccagaagg
     2821 gctggctgtt ggcgttgcct tcggtgcagt ggcggcggac ctaccctctg ctacactagt
     2881 gagtgccatt gctcttgcca tcggcattgg gattcaaaac ttcccggaag gtgtggcagt
     2941 atcggtacct cttcgttcag aaggtatgtc gcgttttcga agctttctct acggtcaatc
     3001 ctctgcatta atcgaacccg tcgcgggtgt gctaggggcg ggagcagttt tattaatgcg
     3061 acccattttg ccctatgccc ttgcatttgc ggccggcgca atgatctttg tggtagtgga
     3121 agagttgatc cccgagtcgc aactggcgaa gaataccgac ttcgccactt caggtgcaat
     3181 gctcgggttt gtaattatga tgattttgga tgttgccttg ggataagatt gcgtttcttc
     3241 gtataccctg ttcataatct tccaaaattc ctcggtaggt cgtgccgtca aaatttcctt
     3301 gcgtgtatcg cgaccccggg agtgtcaatc gcgccgcgtt cgatcacgac tgtgtcatag
     3361 ttcgtgcgaa taccctgctt tgtactgtca gggacaccca gggggagata atgaagaata
     3421 aaacaatttc cgtatgaacg cactaccgcc ttttatctct agtcctccac acgaggtcta
     3481 gaggcaagtg tcccgctttt ttggggacgg atgacccatg acgatccagg ttagagcaac
     3541 gataatgtat acaggcccca tcagaagcat ttgaaggaac gagcgacctc atacaacggg
     3601 ccaattaatt ggactgttca gga
//
LOCUS       DASTJP010000171         3417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_656263, whole genome shotgun sequence.
ACCESSION   DASTJP010000171 DASTJP010000000
VERSION     DASTJP010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656263"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFQ23_05170"
     CDS             <1..396
                     /locus_tag="VFQ23_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9906003.1"
                     /translation="LTSRMGDHRTEEGREFLKSRSPLTYADRIRKPLLIGQGANDPRV
                     KQAESDQIVQAMQSKNIPVTYVLYPDEGHGFARPENNMSFTAVAEAFLAGILEGRFEP
                     IGNDFNGASITVPTGAEQVAGLTEALQKN"
     gene            complement(469..897)
                     /locus_tag="VFQ23_05175"
     CDS             complement(469..897)
                     /locus_tag="VFQ23_05175"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HET9906004.1"
                     /translation="MDFGSINWLAVVACVVASMIIGSVWFNPKTFFPAWWKAIGKSDR
                     DDASGSSIGMGVTWGLIILCSFVQAVFMSLFVNAMGSVMGGTSLASGALAGFMLWLGF
                     VAPSSLTNKLFADRLKAWVLEAGNHLVTFVVMGAIVGAMG"
     gene            complement(983..2392)
                     /locus_tag="VFQ23_05180"
     CDS             complement(983..2392)
                     /locus_tag="VFQ23_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9906005.1"
                     /translation="MTLPVSTAKRVYDNVLGAMGNTPLVRLARIGRDLPVPLYAKLEL
                     MNPGGSVKDRVGAFIIEQAEKRGELKPGGTIVEATSGNTGVGLAIAAALKGYKTIFVM
                     PDKMSNEKILLLRAYGAKVVITPTAVEPDDPRSYYQVAEKFARETPNAILANQYHNPD
                     NPRTHELTTGPELWEQTDGRVTDVIIGMGTGGTITGVGRYLKSKNPNIKIVGIDIEGS
                     ILTEIWQNKGEIPDGAYPKTYKVEGIGEDFLPTALDLSVVDWIERAGDRESFLWARQL
                     VRLEGIFAGGSSGSALAGAIKYCRRLTADRLPVVILPDSGSRYLSKFFDDKWMREFGF
                     LSMEFGETTLGDLLIAKPDKVLYTAMLGDSMRKVVAVMHQHGISQMPVVGKDGALVGL
                     LEEVDLLNHMLDKHEHSQDEPIDSLVQNASAVFPPDTSLEEAMPSLKSGLALIVVESS
                     RPSGILTKIDVLDYVAGKI"
     gene            complement(2438..>3417)
                     /locus_tag="VFQ23_05185"
     CDS             complement(2438..>3417)
                     /locus_tag="VFQ23_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906006.1"
                     /translation="SPHTKIWLGRGLTYHAMFCYRSGDLPHGRMAIEESIAILRQLDM
                     PQFLIEPLVYSSIIYALMGNFPAAQANVNEGVALARQFNDTWFLAVGIFNQGVQFRLL
                     GEPQRAYESIKEALRLWREMGNPPRLTGLAHNFLSAIAIELDKRSEAREYLEESLKLA
                     STNTDRWGLGSALSGFGVLALREGDTVAAENYVGQSIAVFTELGARADLAESLNLFGK
                     LHLYRSEFVEAQVVLKKAIRLGLELNSAPQVCQAALEFADCLVNLDRPEAALTILRAV
                     PAPALKVESVRRRVEQIKGDLEAQYPGNGKDIELPSMTFEELLPRFLKE"
BASE COUNT          883 a          968 c          777 g          789 t
ORIGIN      
        1 ctgacctcac gcatgggtga tcatcgcacc gaagaggggc gagaatttct caagtcgcgt
       61 tcgccgctga cgtatgcgga tcgtatccgc aagccattgc tgatcggaca gggtgccaat
      121 gatccgcgcg tgaaacaggc cgagtcagat caaattgtgc aggcgatgca atcaaagaat
      181 attcctgtga cctatgttct ttatccggat gaagggcatg gcttcgcacg accggaaaat
      241 aacatgtcgt ttacagccgt agccgaagca tttctcgcag gcatcctgga aggtcgcttt
      301 gaaccaattg gtaacgactt caacggtgcc agcattacgg tcccaaccgg cgctgagcaa
      361 gtcgctggct taacagaggc tttgcagaag aattaagcaa atatcattca gaaaaaaata
      421 aagcggatga cttggtcatc cgctttttac taaaaacatc agtcctcttt agcccattgc
      481 acctacgatc gcgcccatca caacaaaggt gacaaggtgg ttgcctgctt cgagcaccca
      541 ggctttcaaa cgatctgcga acaacttgtt cgtcaaactc gagggggcaa cgaaacccag
      601 ccagagcatg aaccctgcca gcgcgccgga agcaagtgag gtgcccccca tcacgctgcc
      661 catcgcattg acgaaaagtg acatgaacac cgcctgaaca aacgaacaga ggatgatcag
      721 cccccaggtc acgcccatcc caatgctgga gccactggcg tcatcacgat ccgatttgcc
      781 aatggctttc caccaggcag ggaaaaaggt ttttggattg aaccaaacac tgccgatgat
      841 catactggca acaacacaag ccacaaccgc caaccaattg atagaaccaa aatccatttc
      901 agcctccatg tgataaattc aattcctgca ggtcaacgct ggaggcagta taactgaata
      961 tttgttctaa gaacaggtgg ggttaaattt ttcccgcgac ataatccagc acatcgatct
     1021 tcgtcagaat tccactgggg cgactgcttt cgactacgat cagggccagc ccggatttca
     1081 acgagggcat ggcttcctcc aatgaagtat caggtgggaa gaccgcgctt gcattttgta
     1141 ccagcgagtc aattggctca tcctgactat gttcatgttt atcgagcata tgattaagca
     1201 ggtctacttc ctcaagtaaa ccaaccagcg cgccatcttt cccaacaacc ggcatctgtg
     1261 aaattccatg ctgatgcatg accgccacaa ccttgcgcat tgaatcaccg agcattgcag
     1321 tgtacaaaac cttgtctggc tttgcaatca acaaatcacc cagtgtcgtt tcgccgaatt
     1381 ccatcgagag gaatccaaac tcccgcatcc acttgtcatc gaaaaatttg gataaataac
     1441 gtgaacccga atcagggagg atcacaaccg gcagtcgatc ggcagtaagc ctgcggcaat
     1501 atttgatcgc tcctgccaac gcagaccctg acgaaccacc cgcaaagata ccttcgagcc
     1561 gcacaagttg acgcgcccac aaaaaggatt cacgatcgcc agcacgctcg atccaatcga
     1621 caacggaaag gtccagcgcg gtcggcaaaa agtcttcacc gattccctcc actttatacg
     1681 tcttgggata ggcgccgtca gggatctcgc ctttgttttg ccagatctct gtcaggatcg
     1741 agccttcgat atcgatccca acgattttga tgttcggatt cttggacttg agataacgcc
     1801 cgactccggt gatcgttccg cccgtaccca ttccaatgat gacgtctgtc acgcgcccat
     1861 cagtctgctc ccacaattcg ggacccgtcg tcagctcatg cgtacgtgga ttatccgggt
     1921 tgtgatattg attggcaagg attgcgttcg gggtttcacg cgcgaacttt tcagccactt
     1981 gataatacga ccttggatca tccggctcaa cggctgtggg agtaataacg accttcgccc
     2041 cataggcgcg caagagcaat atcttctcat tactcatttt gtctggcatc acaaagatcg
     2101 tcttatagcc cttcaaggcc gcggcaatcg cgagtcccac gccggtattt ccggatgtcg
     2161 cctcaacaat ggtgccacct ggcttgagtt ctccccgctt ttcagcttgc tcaatgataa
     2221 atgctccaac acgatccttg actgaccctc ccggattcat taactctaac tttgcataca
     2281 gtggaactgg taaatctctg ccgattcttg caagccgcac cagcggtgta ttccccattg
     2341 cgcccaacac gttatcgtac acacgttttg ctgttgagac tggtaaggtc attctcactc
     2401 ctttgttcgg gatgagaata ttttaccgca ctcaaaatca ctctttcaaa aaacgtggca
     2461 atagctcttc aaacgtcatg gatggcagct caatatcttt tccattacct ggatactgcg
     2521 cttccagatc ccctttgatc tgctccaccc gccgcctgac agattccacc tttaaagcgg
     2581 gtgcaggcac cgctcgaaga attgtcagcg ccgcctccgg gcgatccaaa ttgacgaggc
     2641 agtctgcaaa ttccagagca gcctgacaca cctgaggagc cgaattcaat tccaagccca
     2701 ggcgaatcgc cttcttcagc accacttgag cttcgacaaa ctcggagcga tagagatgca
     2761 gtttaccgaa tagattcagg gactctgcca ggtcggcgcg ggcacctaat tcggtaaaga
     2821 ccgcaatgct ttgaccgacg taattttctg ccgcaacagt atcgccttcg cgtagagcaa
     2881 gtacaccaaa accacttaac gcagaaccca gaccccaacg gtcggtgttc gtagatgcaa
     2941 gcttcaagct ttcttccaaa tattcgcggg cttcactacg tttgtccagt tcaattgcta
     3001 tggcactgag gaaattatga gccagccctg tcaagcgcgg cggatttccc atctcgcgcc
     3061 acaacctgag cgcttctttg atcgattcat aggcacgctg cggttcgccc agcaatcgaa
     3121 attggacgcc ttgattaaag atcccgactg ctaaaaacca ggtgtcattg aactgtcggg
     3181 caagcgcaac accctcattg acgtttgcct gcgcggcagg aaagttcccc atcaacgcat
     3241 aaataatact gctatacacc aatggttcga tcaagaattg cggcatgtca agctggcgca
     3301 aaattgcaat cgactcttcg atagccattc gcccgtgcgg gagatcgcca gaccgatagc
     3361 aaaacatggc atgatacgtg agcccccgcc ccagccagat ttttgtatgt ggagaaa
//
LOCUS       DASTJP010000172         6790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1421968, whole genome shotgun sequence.
ACCESSION   DASTJP010000172 DASTJP010000000
VERSION     DASTJP010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421968"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFQ23_05190"
     CDS             <1..592
                     /locus_tag="VFQ23_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906007.1"
                     /translation="HATAPLSGYEHVISHVLDLIAEQKHRPLAQHGTQVALTTRLTTA
                     AYQIFLDEFEPAELNLANCYPTEEQMKERIESAFRQFDSTGRVAAECWSDYKIKLEAW
                     HAHRSDFESALRDWPAIQNHLQSLVKRPEVAARILNAISSPMRFTELKPPPSTAEVQF
                     AFTNAPLIRRRFTLGDLFIFLNWDQKSLWTQVNKNS"
     gene            610..1296
                     /locus_tag="VFQ23_05195"
     CDS             610..1296
                     /locus_tag="VFQ23_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906008.1"
                     /translation="MQSQLLKPELIVMLTHHDQTVQDALELFLRTKDFPIKHWGFKDV
                     GLPPEEMQNIVNAMKDAGKVTFLEVVSLSEEEGLRGAQLAVQLGFDILMGTVFYPSIA
                     EYLKDKPIQYYPFPGKVHSHPSILDGSIDEIVAHARQLESYGAHGLDLLTYRFNGEAS
                     HLLKLVVDATNIPIVSAGSIASFERIQEVWDAGAWGFTIGSAFFERQFVPDGSFEENI
                     LAVCDWLQKQ"
     gene            1300..2799
                     /gene="xylB"
                     /locus_tag="VFQ23_05200"
     CDS             1300..2799
                     /gene="xylB"
                     /locus_tag="VFQ23_05200"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET9906009.1"
                     /translation="MDCVIGCDVGSQGTKAILLSTTGESIGEVYEGYSIDYPHPLWAE
                     QPVERWVEALSIAIPRLLSETGVAAANIRALSLATQVDGLVAIDSKSQPLYPAIIWMD
                     RRASQQCELVRQSLGDDQIFHLTGLNLDAYHVAPKIRWLAEYQPEIYERAAHFLLPGS
                     YLAYYLTGELAVDYSNASSTLLFDVHSKTWSAKMCEAFDIDMDKLVPLGAANATLGKL
                     RSSIAEQLGLNTNTLVMIGCGDEHAACLGAGVTRPGLVCDIAGTAEPVCASSSEVVFD
                     PTHLVETHCHADPELWLLENPGFVSGGNYRWWRDQFANGESYSALDAEAARIPPGSEG
                     LTFLPSLMGAMAPTWSEAARGTYSGFTLAHTRGHFIRALLEGSAYAVRDITSQMQSIG
                     VELRELRVVSGGAKSTLWNQIKADVTGLQVNVPEITETTALGAAFLALVGIGAYTTLS
                     EASEHIVKIRECIEPNPAAQSIYQEAYERYRDTYFALLPVFEKDAKRTH"
     gene            2806..3618
                     /locus_tag="VFQ23_05205"
     CDS             2806..3618
                     /locus_tag="VFQ23_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HET9906010.1"
                     /translation="MQTQNSLTEVRCFLLDMDGTFYLGEQLLDGSLRFIETLHKQGIE
                     FLFLTNNSSKDSQQYVEKIARLGLSISSEKVLTSGEATVMHIQSQKPGARVYVVGTPA
                     LEEEFIKNGFLITGDQPDFAVLGFDTTLTYDKLWKLCDLVRAGVPYIATHPDYNCPTE
                     TGYMPDIGAMIAFVKASTGREPDLIVGKPNKLFVEKAAERTGIPISEMCMIGDRLYTD
                     IALGSTANIPTILVLSGETKAEEVSASTFQPDYIFQNLGEVAKYLADVKSAK"
     gene            3759..4859
                     /gene="ugpC"
                     /locus_tag="VFQ23_05210"
     CDS             3759..4859
                     /gene="ugpC"
                     /locus_tag="VFQ23_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET9906011.1"
                     /translation="MASVSFENVVKQFGDVKAVNNLNIHVADKEFLVLVGPSGCGKTT
                     ALRSLAGLEEITSGNIKIGDRVVNDVAPKDRDIAMVFQSYALYPHLSVYDNMAFGLKL
                     RKVPKEEIKRRVSEAADILGIQELLQRKPRQLSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVAMRAEISKLHQRLQTTFIYVTHDQTEAMTMATRIAVINKGVLQQLDTP
                     QNLYDHPKNLFVAGFIGSPAMNFFPAKLRKDGDKLVVDAGDFIVAIPSKFAKPYEAHE
                     GKDVIFGIRPENIHDAEYVPTNIDAEKVAVKVDVTELMGNEIFLYLVSGKNTFVARVD
                     PRSKLRVGEQATVAFDMDNIHIFDAATEEAIR"
     gene            5097..6344
                     /locus_tag="VFQ23_05215"
     CDS             5097..6344
                     /locus_tag="VFQ23_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9906012.1"
                     /translation="MDLIRFAGRGISRTDLAEEMGLTRPAVTGIVNDLISNHIILETE
                     NRATASGRPPVVLEINPERGLVAAVDMGAMHLSVALGDFSARILEEAEFPFNIADGPA
                     ECLTVANRILNELLQKRGYSTTDLAAVGVGVPGPVIAEKGMVMAPPIMPGWDRYPIRQ
                     TLEDQWGTAVTLNNDAELGALGEWAYGAGRGEKNLAFIKVGSGIGAGLIINKQIYGGT
                     TGSAGEIGHLTVDENGPLCACGNHGCLEAFAGGHAIAAQAKKLAASGKRTLLSETNIE
                     SITARDVVEAARRGDLAAQEILKRSGTFVGIAIAGLINLINPSTVIIGGGVAQVGDLL
                     TSPIRQAVHARSLRASEHGVRITMAMLGRRSSLIGALVQAISIAIHNAVESKGQSVKS
                     RSLQLTKQPNPLSNEMEVITSKQ"
     gene            6417..>6790
                     /locus_tag="VFQ23_05220"
     CDS             6417..>6790
                     /locus_tag="VFQ23_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906013.1"
                     /translation="MKKKLSLLFSFAVIASIVLTACGGAPAATEPAATEPAATEPGVA
                     IQPSTEEPATQAPVTVSEFSEAPMLAELVASGDLPPVEERLPVKDDIFVVTPVDGIGE
                     YGGTWHEATWWQGMGNIEMTVY"
BASE COUNT         1729 a         1655 c         1733 g         1673 t
ORIGIN      
        1 gcatgccacc gccccacttt caggatatga acatgttatt tcacatgtgc tggatttgat
       61 cgctgaacaa aagcatcgac cgctcgcaca acacggaaca caagtagcgt tgacaacccg
      121 gctcaccacg gccgcatatc aaattttcct cgatgagttc gagccagccg agctcaacct
      181 cgcaaactgc tatccaactg aggaacagat gaaggagcgg attgaaagtg cttttcgtca
      241 attcgactct acgggacgcg ttgcggcaga gtgctggtca gactataaaa tcaagctgga
      301 agcctggcat gcacatcgtt ctgactttga atctgccctg cgcgactggc ccgcgattca
      361 gaatcactta cagtcccttg tcaaacgacc cgaagtggca gcacgtattt tgaatgccat
      421 ttcatcgccg atgcgcttca ctgagctaaa accaccgcct tccacggctg aagtccaatt
      481 tgcttttacc aacgcacccc tcatccgtcg acgctttacg ctgggtgatt tgtttatctt
      541 cctaaattgg gatcagaagt ccctgtggac gcaggttaat aaaaattctt gagaaaaggc
      601 tttaaaacta tgcaatccca actcctcaaa cccgaattaa ttgttatgct cacccaccat
      661 gatcagaccg ttcaagatgc cctggaatta tttctgcgaa cgaaagattt tccgatcaag
      721 cattggggtt tcaaagatgt ggggttgccc ccggaggaga tgcaaaatat tgttaatgcc
      781 atgaaagacg ccgggaaagt tacatttctc gaagttgtga gtctttcgga agaggaagga
      841 ttgcgcggcg cacaactggc ggttcagttg gggttcgaca tcctgatggg caccgtgttc
      901 tatccatcaa tcgcagagta tctgaaagac aaacccattc aatattatcc atttcctggc
      961 aaggtccata gtcaccccag tattcttgac ggctcgatcg atgaaattgt cgcccatgca
     1021 cgccaacttg aatcgtatgg cgctcatggg ctggatttgc tcacctatcg ctttaacggc
     1081 gaagcctctc acctgctaaa actggttgtt gatgcgacaa atattcccat cgtgtcggcc
     1141 ggtagcatcg catcctttga acgtatacaa gaagtctggg acgccggagc atgggggttc
     1201 acaattggtt ctgccttctt cgagagacag tttgttccgg atggatcctt tgaagagaac
     1261 attctggcag tatgtgattg gctacaaaaa caataagcca tggactgtgt tatcggatgt
     1321 gatgtcggtt cacaggggac aaaggccatc ttactctcaa caacaggcga atccatcggt
     1381 gaagtttacg agggctattc gattgactat cctcatccgc tctgggcaga acagccggtg
     1441 gagcgatggg tcgaggcgct cagtatcgct atcccacgtc tgctttctga aaccggagtt
     1501 gccgcggcaa atatccgtgc tttgagtctg gcaacgcagg tagatggtct tgtcgcgatt
     1561 gactccaaaa gtcaaccgtt gtacccggct attatctgga tggaccgccg ggcaagccaa
     1621 caatgtgaac ttgtccgtca aagcctgggc gatgaccaaa tctttcattt gaccgggcta
     1681 aatctcgatg cctatcatgt cgctcctaag attcgttggc tcgcggaata ccaacctgaa
     1741 atttatgagc gcgcggcgca ctttctactg ccgggctcct acctagccta ttatctgact
     1801 ggtgaattgg cagtagatta ctcgaatgct tcatcaacgt tgttgttcga tgtgcacagc
     1861 aagacatggt cagccaagat gtgcgaggca ttcgacatcg atatggataa gctggttcca
     1921 cttggtgcag ccaatgcgac tctgggaaaa cttcgctcaa gcattgccga acaacttggc
     1981 ttgaatacaa acacactggt gatgatcgga tgtggtgatg aacacgccgc atgtctgggc
     2041 gcgggcgtaa cgcgtccggg cttggtatgt gatatcgcag gcacggctga accggtctgt
     2101 gcatccagtt cagaggttgt cttcgatccg actcacctgg ttgaaaccca ttgtcatgct
     2161 gaccctgagt tgtggttgtt agagaatccc gggtttgtat cgggtggtaa ttatcgctgg
     2221 tggagagatc agttcgcaaa tggagaatca tacagcgcgc tggatgctga agctgcgcgt
     2281 attccgccag ggtcagaggg attaacgttt ctcccgagct tgatgggtgc catggctccc
     2341 acctggagcg aagcggcgcg cggtacgtat tctggcttca ctctggccca cacgcgcggg
     2401 cactttatcc gtgcgctgct tgaaggctca gcgtatgcgg tgcgcgatat cacatcccaa
     2461 atgcagtcta tcggtgttga gttgcgcgag ctgcgagtcg tgagtggagg cgcaaagagc
     2521 acactatgga atcaaatcaa ggcagacgtt actggtctac aggtgaatgt acctgagatt
     2581 actgagacca ccgcgctcgg cgcagcattc ctggctctag tggggattgg agcttacacc
     2641 actctttctg aagccagcga gcacatagtg aagatccgtg agtgcattga gccaaaccct
     2701 gccgcgcagt ctatttacca ggaagcctat gaacggtatc gagacactta tttcgctttg
     2761 ttgcctgttt ttgaaaagga cgcaaagcgt acacattaga ctattatgca aacacaaaat
     2821 tcattaaccg aagttcgttg ctttctgtta gacatggatg ggacttttta tttaggcgaa
     2881 cagttactcg atggttcact acggttcatc gaaaccttgc acaaacaggg aatcgaattc
     2941 ttattcctta ccaataactc atccaaagac agtcagcaat atgtagaaaa aatcgctcgc
     3001 ctggggttat ctatttctag cgaaaaggtt ttgacttccg gtgaagcgac ggtgatgcac
     3061 atccaaagtc aaaagccagg ggcgcgagta tatgtggtcg gtacccccgc gcttgaagaa
     3121 gaattcataa agaatgggtt tcttatcacc ggggaccaac cggacttcgc cgtattgggc
     3181 ttcgacacaa ccctgaccta tgacaagctg tggaagttat gtgacttggt acgtgctggg
     3241 gttccttata tcgcaactca tcctgattac aactgcccta ccgaaacggg ctacatgccc
     3301 gatatcggcg caatgatcgc ctttgtcaaa gcatccacag gtcgcgagcc tgatctgatc
     3361 gttggcaagc ccaataaatt gttcgtcgag aaagctgctg agcgaactgg gataccaatt
     3421 tcagagatgt gcatgattgg ggatcgactt tacacagaca tcgcactcgg atccacagca
     3481 aatattccta cgattctcgt tctcagcggt gaaaccaagg ctgaagaagt gtctgcatca
     3541 acgtttcaac cggattacat attccaaaat ctcggagagg tagcaaaata cctcgctgat
     3601 gtgaaatctg ccaaataaat tccaccctgg gggttccaaa tttttacgcc atacaatctt
     3661 gctcgcgggc acataactag tgcagaattt gtgattcctt atctgaaaat gcgtgtataa
     3721 tcgaatcact atattcccac gaaacggagg aaagacccat ggcaagtgtc tcatttgaaa
     3781 atgtcgtcaa gcagttcggc gacgtgaagg ctgtcaacaa cctgaatatt catgttgctg
     3841 acaaagagtt tcttgttctc gtaggtccgt ctggttgtgg aaagaccacc gcgttgcggt
     3901 cgctggcagg gctggaggaa atcaccagtg gcaatatcaa aatcggtgat cgcgtcgtca
     3961 acgatgtagc gccaaaggat cgtgatatcg ccatggtctt tcaatcgtat gctctatacc
     4021 cccacctttc tgtatatgac aatatggcat ttggactgaa acttcgcaaa gtgcccaagg
     4081 aagagatcaa gcggcgcgtt agtgaagcag cagatattct cggcatccag gaactgcttc
     4141 agcgcaagcc gcgtcagcta tccggtggtc aacgtcagcg tgtggctgtg ggccgcgcga
     4201 tcgttcgtga gccaaaggtc ttcttgtttg acgaacctct ttcgaacctg gatgcgaagc
     4261 tgcgcgtcgc tatgcgcgca gagatcagca aactgcatca gcgcctgcag accaccttca
     4321 tctatgtgac gcatgaccag accgaggcca tgacgatggc aacacgcatt gcggtcatta
     4381 acaaaggcgt gcttcaacaa ctcgataccc cgcaaaatct atatgaccat ccgaagaacc
     4441 ttttcgtggc aggcttcatc ggctccccgg caatgaactt cttccccgcg aagctccgaa
     4501 aggatgggga caaacttgtt gtcgatgccg gtgactttat cgttgccatt ccatcgaaat
     4561 ttgccaaacc atacgaagct catgaaggca aggatgtgat ctttggcatc cgccctgaaa
     4621 atatccacga tgcagagtat gtgccgacca acatcgatgc cgaaaaggta gcagtaaaag
     4681 tagatgtgac cgagttgatg ggtaacgaga ttttcctcta tctcgtgagc ggaaaaaata
     4741 cgtttgtggc ccgcgtcgac ccccgttcta agctgcgcgt tggtgaacag gctactgtgg
     4801 catttgacat ggacaacatt cacattttcg atgccgccac tgaggaagcc attcgataat
     4861 ggaacgagag tattcttagc gtacaattca ggtgtagcct tggggttaca cctgaatttc
     4921 ttaatcaaga aaactttaag attggacttt gttaaaacac ttgataatct cccgatttat
     4981 gctatcatag ccatgccaaa tcagtagtac atatttccta ataaggataa accttgtcgg
     5041 ctttctactg gcttccggtc aaccagaatg ttaggaattt taataaacac gcggcagtgg
     5101 atttgattcg tttcgcgggc agagggattt cacggacaga tcttgctgaa gaaatggggt
     5161 taacgcgacc tgccgtcact ggcattgtca atgatttaat ttcaaaccac attatcctgg
     5221 aaacagaaaa ccgagctaca gccagtggtc gcccacccgt tgtactggaa atcaatccgg
     5281 aacgaggtct ggttgccgcc gtagacatgg gagctatgca cttgagtgtt gccctgggag
     5341 atttttctgc ccgaattcta gaagaagcag aattcccatt taatatcgca gatgggcctg
     5401 cagaatgtct tacggtggca aatcgtattc tgaacgaatt attacaaaag cgcggctatt
     5461 ccacgactga tctcgcagcg gtcggagtgg gagtgccggg gccagtgatt gccgaaaaag
     5521 ggatggtgat ggctcccccc atcatgccgg gctgggatcg atatccaatc cgtcagactc
     5581 ttgaagatca atggggaacg gctgtgaccc tcaacaatga tgcagagttg ggagccttag
     5641 gtgaatgggc gtacggcgcg gggcgtggcg agaaaaatct cgctttcata aaagtgggga
     5701 gtgggatagg ggcaggtctc attatcaata aacaaatcta tgggggcact accggctcag
     5761 ctggcgaaat cggacacttg accgtggatg aaaacggtcc gctgtgcgcg tgtggaaatc
     5821 acggatgtct ggaagccttt gcaggcggac atgccattgc cgctcaggct aagaaactgg
     5881 cggcttccgg aaaaagaact ctgctttccg aaacgaatat cgagtcaatc actgctcgtg
     5941 atgttgtgga agctgctcga agaggtgacc tggcagccca ggaaatctta aaacggtcgg
     6001 ggacatttgt aggaattgcg atcgcgggtc tcattaacct gatcaatccc agcactgtca
     6061 ttatcggtgg tggtgttgca caggtcggtg atttgctgac cagcccgatt cgtcaggcgg
     6121 tacacgcaag atcgttacgc gcctctgagc atggtgtgcg tatcacgatg gcaatgcttg
     6181 gacgccgttc gtccctgatt ggtgccttgg tgcaggcaat cagtattgcc attcataacg
     6241 cggtcgaaag caaggggcaa agtgtgaaaa gtcgttcgct acagctcaca aaacaaccga
     6301 accccttatc aaatgaaatg gaggtgatta catcgaagca gtaacctgtc tatgattgaa
     6361 taaccaattg cgtaagtaaa aaatttcatc cactcatatt aaggagaaga gaaataatga
     6421 aaaagaaact ttcactgctc ttcagttttg cggtgatcgc cagcatcgtt cttactgctt
     6481 gtggtggtgc acccgccgcg actgaacccg ctgccaccga acccgccgcg actgaacccg
     6541 gtgttgctat ccaaccctct acggaagaac ctgcaactca ggctcctgtt acagttagtg
     6601 aattttcaga agcacccatg ctggcagagt tggttgcctc tggcgacctg ccccctgtcg
     6661 aagagcgcct gcctgttaaa gatgacatct ttgtcgtgac cccggtcgac ggcatcggtg
     6721 agtatggtgg tacctggcac gaagcaacct ggtggcaagg catgggcaat atcgaaatga
     6781 ctgtctatga
//
LOCUS       DASTJP010000173         5677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_903396, whole genome shotgun sequence.
ACCESSION   DASTJP010000173 DASTJP010000000
VERSION     DASTJP010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903396"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1178)
                     /locus_tag="VFQ23_05225"
     CDS             complement(9..1178)
                     /locus_tag="VFQ23_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9906014.1"
                     /translation="MELNPVLSMLGRRLRLAVIGGGPGSFIGAMHRQAARLDDRYEIV
                     TGVLSSNLEKSNKAAQELGMDANRLYASVEEMLDKESIREDGADVVAIMTPNDSHYEY
                     AMYALERGFDIICDKPMTNTLEEAEALHKKVQETGLIFCLTHNYTGYPMVRQAKAMVQ
                     DGQLGTVRLVQVEYVQGGKADESKPDPSESWRFDPVRGGPSLVMGDIGSHAHNLVRFI
                     TGLEVDEVLSEIGNIVPGRAVHDYGGALLRFNNGARGSYWVTQAAAGVENCLRIRVSG
                     TQGSLEWMQEFPQALTFKPLGAPSQNRTPNGPGTLPLSKHSSRIVAGHPEGFHEGFAN
                     IYSDAAEAIAARRSGKTPNPLATYFPNSFDGLMTLRFVFSAIESSKANGKWTKCS"
     gene            complement(1180..1647)
                     /locus_tag="VFQ23_05230"
     CDS             complement(1180..1647)
                     /locus_tag="VFQ23_05230"
                     /inference="COORDINATES: protein motif:HMM:NF002696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HET9906015.1"
                     /translation="MDDLLKQLLDEEQELQFSTFNEKTAWQVGSLLVEYASSQGLPVT
                     IDITRGQHQLFHASLSGTSADNDEWVKRKVRLVYRFGHSSFYLGQMLKSKGKRIEEAY
                     LISENEYAPHGGCFPVIVRGTGMVGTITVSGLPQEEDHKLVVQAIRNYLAQEQ"
     gene            complement(1729..2940)
                     /gene="mmsB"
                     /locus_tag="VFQ23_05235"
     CDS             complement(1729..2940)
                     /gene="mmsB"
                     /locus_tag="VFQ23_05235"
                     /inference="COORDINATES: protein motif:HMM:NF040906.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HET9906016.1"
                     /translation="MKIPAAINEVTSSNRIVGTLTSIFRSNIREYGMLIALIAVMIFF
                     QIRTDGILMKPINITNLVLQNSYVIIMALGMLLIIVSGWIDLSVGSVAAFVGAIAGVM
                     MVKWGWNWAVTIGICLLIGGLIGAWQGYWVAYVKIPAFIVTLAGMLIFRGLTLVMVRG
                     EAIGPFPVQFGRMSTGFIPDFFNMEGFHLTTIVIGVIVSILLIWFDVRTRRNQLKYGF
                     EVTPLYFFVAKQILITAAIIGFCYLMASYKGIPNVLVIMFPFVAVYAFLTNRTVIGRR
                     IYAMGGNEKAARLSGVNTPRLLFLTFVNMGALAALAGLIVAARLNSATPNAGEGFELD
                     VIAACFIGGASASGGIGTIIGAVVGAFFIGVMNNGMSILGVGIDWQQAIKGFVLLIAV
                     FFDVYNKNKTE"
     gene            complement(2937..4475)
                     /gene="mmsA"
                     /locus_tag="VFQ23_05240"
     CDS             complement(2937..4475)
                     /gene="mmsA"
                     /locus_tag="VFQ23_05240"
                     /inference="COORDINATES: protein motif:HMM:NF040905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9906017.1"
                     /translation="MSDVILEMRNITKTFPGVKALDNVTFSVKQGEIHALVGENGAGK
                     STLMNVLSGVYPYGSYEGEIHFEGQEVRFQDISQSEEMGLVIIHQELALVPYLSITEN
                     LFLGNERARHGIIDWNESVSRTKELLDQVGLHESPNTLITNMGVGKQQLVEIAKALAK
                     KVKLLILDEPTASLNQNDSEKLLELLLQFKARGIASILISHKLSEVSKVADSITILRD
                     GATIETLDTHKDVITEDRIIRGMVGRDMTHRFPPRESKIGDVIFEVKDWSAFHPLHAD
                     RKVSDNVNIHVRKGEVVGIAGLMGAGRTELAMSIFGRAYGAHITGRAYKNGKEIDVST
                     IDKAIANGLAYATEDRKAYGLILIEEVKDNITLSNLEEISDAGVINEPRELTVTREYR
                     NKLNIKCSSILQRAVNLSGGNQQKVVLSKWLFANPDILILDEPTRGIDVGAKYEIYTI
                     INRLASEGKGIILISSELPEILGMSDRIYVMRNGKIVGELPAQEASQEKIMTYIMRKQ
                     EVPQ"
     gene            complement(4583..5665)
                     /gene="chvE"
                     /locus_tag="VFQ23_05245"
     CDS             complement(4583..5665)
                     /gene="chvE"
                     /locus_tag="VFQ23_05245"
                     /inference="COORDINATES: protein motif:HMM:NF040907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9906018.1"
                     /translation="MSKKFVFTLVAVMVLASFILTACGGAAAPADDKKLVGISMPTKT
                     STRWISDGESMVKSFEALGYATDLQFADNDIPNQIAQIENMVTKGADVLVIAAIDGST
                     LSDVLAKAKEQGVLVVAYDRLITKTPDVDYYATFDNFGVGVIMGQQIEEGLDLKNAEG
                     PFNIELFGGSPDDTNAFYFYDGAMSILQPYIDSGKLVVQSGQMGMDVVGTLRWDGTVA
                     QARMENLLSANYTDKRVDAVLAPYDGLSRGIIAALKGVGYGSADQPMPVITGQDAEVA
                     SVKAMIAGEQTYTIFKDTRELAAQTAKMVDQALKGETVDINDTTTYDNEVKVVPSYLL
                     TPHSVDISNYETLLIESGYIKPEDLQ"
BASE COUNT         1411 a         1526 c         1311 g         1429 t
ORIGIN      
        1 tggatacctt aggagcactt tgtccacttg ccattggctt tactggattc aatggcgctg
       61 aaaacaaagc gcaacgtcat cagaccatcg aacgaattgg gaaaatacgt cgctaaagga
      121 tttggcgttt tcccggaccg acgggcagcg attgcctcgg ctgcgtctga ataaatattc
      181 gcaaagcctt cgtggaatcc ttcagggtga cctgccacaa tacgtgatga atgttttgac
      241 agtggtaagg tgccgggtcc gttgggggtt ctgttttgtg atggcgcgcc taatggcttg
      301 aaggttagcg cctgtggaaa ttcctgcatc cattcgaggc tgccctgcgt gccgctaaca
      361 cgaatccgca gacaattctc cacacctgcg gcggcttgcg tcacccagta gcttccacgt
      421 gcgccgttat tgaagcgcag caaagcccca ccataatcat gtactgcccg ccccggcaca
      481 atgttcccga tctcagacaa tacttcgtca acctcaagtc ccgtaatgaa gcgcaccagg
      541 ttgtgcgcgt gcgaaccaat atcgcccatc accagggacg gacctccacg aacgggatca
      601 aacctccagg actcagacgg atctggtttg ctttcgtctg ctttgccccc ctgaacatac
      661 tccacctgaa cgagcctgac cgtgcctaac tgcccatcct gaaccatggc ctttgcctgc
      721 cgcaccattg ggtaacccgt gtaattgtgt gtgagacaaa aaatcagacc ggtctcctga
      781 actttcttat gcaaggcttc tgcttcttcg agcgtgttgg tcatgggctt gtcgcagatg
      841 atatcgaagc cacgctccag ggcatacatg gcatattcat agtgactgtc gttcggggtc
      901 atgatggcga ccacatctgc accatcttca cggatggatt ccttatcgag catctcctcc
      961 acgctggcgt acaagcggtt cgcatccatg ccaagttcct gggcagcttt attcgatttt
     1021 tcaagattag atgaaagaac ccctgtgacg atttcatatc ggtcatcaag gcgagcagcc
     1081 tgtcgatgca tcgctccgat aaatgaaccg ggtccaccgc caatgaccgc caaacgaagc
     1141 cttcgtccaa gcatcgatag tactggattt agttccatct cattgctcct gtgccagata
     1201 gtttcgaatt gcctgaacga ccagtttatg atcttcctcc tgcggcagcc ccgaaaccgt
     1261 gatcgtgcca accatcccgg tccctcggac gattacagga aaacaaccac catgaggagc
     1321 atattcattt tcagagatta agtaggcctc ttcaatgcgc ttgcctttgc tcttgagcat
     1381 ttgcccaagg taaaaggaac tatgtccaaa tcgatataca agacgcacct tgcgtttcac
     1441 ccattcatca ttatcggcgg aggttccgct tagactggca tgaaaaagct gatgttgtcc
     1501 gcgggtgata tcaatcgtta caggaagtcc ctgactcgag gcatattcaa caagtagact
     1561 accaacctgc caggccgtct tttcattaaa tgttgaaaat tgaagttcct gttcttcgtc
     1621 gagcaattgc ttcagcaaat catccatgta aatatcctgt gaaaataaag gtgacagcct
     1681 cttcataaga aaccgtcacc atggcctcca attacaatcc aacgtctgtt attcagtctt
     1741 attcttgtta tacacatcaa agaaaacagc aattaacagg acaaagcctt tgatcgcctg
     1801 ctgccaatcg atgccaacgc caaggatgga catgccgttg ttcatcacac caatgaagaa
     1861 tgctcccacc actgcgccga taatggtacc gataccaccg ctggcagaag cgccgccgat
     1921 gaagcaggct gcgatcacat ccagctcgaa accctcaccc gcattgggtg tggcgctgtt
     1981 aagacgggct gccacgatta atcccgccag cgcagccagc gcgcccatat tgacgaaggt
     2041 gaggaacaat agacgagggg tattgacacc agagaggcgg gcggcttttt catttccacc
     2101 cattgcgtaa atgcggcgtc cgatcacggt gcggtttgtc agaaacgcat acacagcaac
     2161 gaaggggaac atgatcacca ggacgttcgg gatgccctta taggatgcca tcaaatagca
     2221 gaagcctatg atcgctgcag taattaatat ttgtttggct acaaagaaat aaaggggtgt
     2281 gacctcaaat ccatatttca attggtttct tcgtgtgcgc acatcaaacc agatcaacag
     2341 gatcgaaaca atgacaccaa tcacaatggt cgtaagatgg aaaccttcca tattaaagaa
     2401 gtctgggata aaacctgtgc tcatccgtcc gaactgcacc gggaacgggc caatcgcctc
     2461 cccgcgcacc atcactagtg taagaccgcg gaagatcagc atgcccgcca gagtaacgat
     2521 aaacgccggg atcttcacat acgccaccca atagccctgc catgcgccga tgagaccacc
     2581 gatcaacagg cagatcccaa ttgtcaccgc ccaattccag ccccacttga ccatcatgac
     2641 tccggcaatc gcaccgacga aagcggcaac agaacccacg gagaggtcta tccagccgga
     2701 aacaatgatc agcagcattc ccaatgccat gatgatcaca taactgttct ggagaacgag
     2761 attcgtgata ttgattggct tcatcaagat gccgtctgtc cggatctgga agaagatcat
     2821 gaccgcgatc aatgcgatca acatgccata ttcacggata ttgctccgga aaatactggt
     2881 cagggttccc acgattctgt tactgctggt tacctcatta atggccgcgg gaattttcat
     2941 tggggtacct cctgctttct catgatgtac gtcatgattt tttcctggga tgcctcctgg
     3001 gccggaagtt cgcctacaat ttttccattc cgcataacat aaatacggtc agacataccc
     3061 aaaatttcag gcaattccga tgagataaga atgatccctt ttccctcgct tgccaggcga
     3121 ttaataatcg tataaatctc atacttagcc cccacatcaa tcccgcgggt cggttcatcc
     3181 aaaatcagaa tatcaggatt ggcaaacagc catttactca acaccacttt ctgctggtta
     3241 cctcccgaaa ggttgaccgc ccgctgcaaa atactggaac acttgatatt taacttattt
     3301 cgatattcgc gggtaacggt taattctctg ggttcgttga tcactccggc atctgaaatt
     3361 tcttccaggt tggatagcgt gatgttatcc ttcacctctt caatcaggat caatccatag
     3421 gctttgcggt cctcagtggc atatgcaaga ccgttggcaa ttgccttatc gattgtactg
     3481 acatcgattt ccttgccgtt tttataagcc ctgccggtga tatgtgcacc gtaggcgcga
     3541 ccgaaaatac tcatcgccag ttccgttcgc cctgctccca tcaacccggc gatgccaacg
     3601 acttcccctt tgcgaacatg aatatttacg ttgtcgctta ccttgcgatc tgcatggaga
     3661 ggatgaaagg cgctccaatc cttgacctcg aaaattacat ccccaatctt cgactcacgc
     3721 ggcggaaagc ggtgagtcat atcgcgcccc accatgccgc gaatgattcg atcttcagtg
     3781 atcacatctt tatgggtatc cagggtttcg atcgtggcgc catcgcgaag aatggtaatc
     3841 gagtccgcca ctttggaaac ttcgcttaac ttgtgagaga tcaaaatgga cgcaatccca
     3901 cgcgctttaa attggagcag cagttccaac agcttctcac tatcgttctg gttcagagag
     3961 gcagtcggtt cgtccagaat gagcagctta acctttttcg ccagcgcctt ggcaatttct
     4021 accaactgct gtttgccaac acccatgttc gtaattaagg tgttggggga ttcatgcagc
     4081 cccacctggt cgagcaattc ttttgttctc gaaacagatt cattccaatc gatgatgcca
     4141 tgcctggcac gttcattccc gagaaaaagg ttttcggtaa tagagagata gggaacaaga
     4201 gctaattcct gatggatgat gaccagtccc atttcttctg attgactgat gtcttgaaag
     4261 cggacttcct ggccttcgaa atggatttcc ccttcgtacg aaccatacgg atagacacca
     4321 ctcaacacat tcattaatgt ggatttgccc gctccgtttt cccctaccag ggcatgaatc
     4381 tcgccttgtt ttacgctgaa ggtcacattg tcaagcgcct tcacgccggg gaacgtcttt
     4441 gtaatatttc gcatttcgag gattacatca gacatggatt cttgtcctta atcggatttt
     4501 tagctattct actgcaagac cttaacaaat aagagatggg tgtgtgagaa cgtattgtct
     4561 cacacaccca ttcagaaact tcttattgca gatcttcggg cttgatataa ccactctcaa
     4621 ttaataacgt ctcgtagttg gaaatatcca cgctgtgggg ggtcagtaga taggaaggaa
     4681 caactttgac ttcgttgtca taggtcgtgg tatcgttgat atccacggtt tcacccttca
     4741 atgcctggtc caccatcttg gcggtctggg cagccagctc acgagtatct ttgaagatgg
     4801 tgtaagtctg ttcgccagca atcatggctt tgacggaggc aacttcggca tcctgaccgg
     4861 tgatgaccgg cataggctgg tctgctgagc cataccctac gcccttcagg gcggcgatga
     4921 tcccgcggct gagtccatcg tatggggcaa ggacagcatc cacgcgcttg tctgtatagt
     4981 tggcgctgag caggttttcc atacgggcct gggcgactgt gccatcccag cgcagggtac
     5041 ccactacatc catgcccatc tggccagatt gaactacaag cttaccgcta tcaatatagg
     5101 gttgcaggat cgacatagca ccatcataga aataaaaggc attggtgtcg tcgggcgatc
     5161 cgccgaacaa ctcgatgttg aagggacctt cagcattttt caggtccagc ccttcttcga
     5221 tctgctgtcc cataatcaca ccgacgccaa agttatcgaa cgtagcgtag taatcgacgt
     5281 caggagtctt tgtgatcagg cggtcgtatg caaccaccag aacgccttgt tcctttgcct
     5341 tggcaagaac gtctgaaagg gttgagccat caatggctgc gatcacgagt acatcggcac
     5401 ccttggtaac catgttttcg atctgagcga tctggttcgg gatgtcgtta tccgcgaact
     5461 gcaggtcggt agcatagcca agcgcttcga aggatttgac catgctctca ccatctgaaa
     5521 tccaacgggt ggaggtcttg gtcggcatgc tgatgccaac gagtttctta tcgtcagcag
     5581 gggcagcggc tccgccacat gccgtaagaa tgaagctggc cagcaccatg actgctacca
     5641 gtgtaaagac gaacttcttg gacattttct tctcctt
//
LOCUS       DASTJP010000174         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_611968, whole genome shotgun sequence.
ACCESSION   DASTJP010000174 DASTJP010000000
VERSION     DASTJP010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611968"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFQ23_05250"
     CDS             complement(<1..282)
                     /locus_tag="VFQ23_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906019.1"
                     /translation="MNRTLAFVYGTVVYLIGLVVTFYTIGFVGNLVVPKTIDSGEPAS
                     FAQALLIDTLLLALFGIQHSLMARPGFKQWWTRLVPEPVERSTYVLFTSL"
     gene            complement(348..617)
                     /locus_tag="VFQ23_05255"
     CDS             complement(348..617)
                     /locus_tag="VFQ23_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906020.1"
                     /translation="MLLKDAKSEALAPLIAEITQLSILQTFPGNIVSGIHATLIARIN
                     QGPIFVAGNEITFEAESTGNLLLRMNDKSTSDNSGSIVVSVKVMP"
     gene            complement(617..1084)
                     /locus_tag="VFQ23_05260"
     CDS             complement(617..1084)
                     /locus_tag="VFQ23_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906021.1"
                     /translation="MNRWRKRLSALVVVPGCFHLLTYQMEAGFQIMEPKDIPHQTPIV
                     SSALNSFFHEKLVDHLLKNRAGIEKVQRVHLFRKSNNFTSDDYFTFTVKRQWPLETIM
                     ADDFHPELINTIADFSESVFFSRNYYNQVSSETDLTFQELPVISLIPLGEEDV"
     gene            complement(1197..1775)
                     /locus_tag="VFQ23_05265"
     CDS             complement(1197..1775)
                     /locus_tag="VFQ23_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906022.1"
                     /translation="MSSTKAEIKKHLDQLVGLPLSIARRAANMLVLHFGTIREVEGRR
                     LSRKKRKDKKGTVGDFALHIQCPWRLENSEGIITGRGDLYLSAETGNYFDDDEEDDSF
                     DKQGMNLQDRKMGELLQGIDPITGSYMNVTNFLIVESIESDDFGEATIYLSGGYRLRI
                     FPSSSTVEHWRLFQPATEEKHFVVEGSEVEAE"
     gene            complement(1860..2219)
                     /locus_tag="VFQ23_05270"
     CDS             complement(1860..2219)
                     /locus_tag="VFQ23_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906023.1"
                     /translation="MNSNTSAPFSGNVSDVSKDTRGWFLGHFMPGEENPLHTEEVELK
                     WYTHAKGETRHEWAPGNPVRTLNILIRGHFVLLFPDQEVPLEKEGDFVIFGPGVPHSF
                     RCEEESLVITVRWPSAP"
     gene            complement(2408..2980)
                     /locus_tag="VFQ23_05275"
     CDS             complement(2408..2980)
                     /locus_tag="VFQ23_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906024.1"
                     /translation="MDANIINILIVSLALCSPFIFSFLIVAILSPRFKALSERNGWTV
                     QPHPKDHRDHLFRGQQNGIQWEMEYYNYDHYRKLPPGSTPYIQVIAWTTKSVVLPTHP
                     ILIYPRRDKLKQIVHPRLALENEHSFKNPADKANMENMLSDLPEKSIGTDEFRELFLL
                     RTEREVLPHKLITSLQLELSPGPKYSLSDR"
     gene            complement(3049..3540)
                     /locus_tag="VFQ23_05280"
     CDS             complement(3049..3540)
                     /locus_tag="VFQ23_05280"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906025.1"
                     /translation="MKKYSKEDQKLMATWAADCAERVLPFFEEAYPEDDRPRKALETC
                     RTWVRTGVFKMAEIRGASLSSHAAARDVKDNDPALFAARAAGQAVATAHVAQHGYGGA
                     LYALKAIAAKDPANAERNVAEERDWQARHLPKHLREEIMSRIVVEKRRKGIFVKLIKD
                     ENY"
BASE COUNT          945 a          877 c          857 g         1035 t
ORIGIN      
        1 aaggctggtg aacagaacgt aggtactgcg ttccaccggc tcgggaacca ggcgagtcca
       61 ccactgcttg aagccgggtc gcgccatgag gctgtgctga atgccgaaga gtgcgaggag
      121 gagagtgtcg atcagcaggg cttgagcgaa ggacgcaggc tcaccggaat cgatggtttt
      181 gggcaccacc agattaccga cgaacccgat cgtatagaaa gtaacgacca gcccaatcaa
      241 atagacgacc gtcccatata caaacgcgag tgttcgattc atatcccagc ctccttttta
      301 gaactgttgt cgcattcata aaatcgctat cgaaatctct tcaggcttca tggcataact
      361 ttcactgaaa ccacaatgga accggaatta tcggaggtgc ttttgtcgtt cattcgaagc
      421 agtagatttc cggtgctttc tgcctcaaag gtgatttcat tcccagctac aaaaattgga
      481 ccctggttga tccgcgcaat taaggtagcg tgaatgccgc tgacgatgtt accaggaaaa
      541 gtttgaagga tggatagctg agtgatctct gcgatgagtg gagccagggc ttcggacttt
      601 gcatctttta gtaacactat acatcctctt ctccaagtgg aataagagaa attactggca
      661 attcttgaaa cgtaagatca gtttcagacg agacttgatt gtagtaattt ctggagaaga
      721 atacagactc agaaaaatca gcaatcgtgt tgataagctc aggatgaaaa tcatctgcca
      781 ttatcgtttc cagaggccat tgccttttga ccgtgaaggt gaagtagtcg tctgatgtga
      841 aattattcga tttccgaaaa agatgaactc gttggacttt ttcaattcct gctctattct
      901 tcagaagatg atccaccaat ttttcgtgaa aaaatgaatt caaagccgat gagacgatgg
      961 gagtttgatg aggaatatcc tttggctcca taatctggaa ccctgcctcc atttggtacg
     1021 tcaacaggtg gaaacagcct ggtacaacca caagtgcaga aagtcttttt ctccagcgat
     1081 tcacaaggaa cttcctaagt tattccgctg gttgaagcgt ggagcaaatg ctgccgcagg
     1141 attctcaatc tttcgatgtc acattgttgg gttacatttt gcaaatctct tctatcctat
     1201 tcagcctcaa cttctgagcc ctctacaaca aaatgttttt cctctgtggc aggctgaaat
     1261 aatctccaat gctccacagt ggaactggat ggaaatattc tcagccgata cccaccagat
     1321 agataaatcg ttgcttcgcc aaaatcatca gactcgatac tctcaacaat aaggaaattg
     1381 gtcacattca tgtatgagcc tgtgatggga tcgatgcctt gtaatagttc gcccatcttc
     1441 ctatcctgta aattcatgcc ctgtttgtca aacgaatcat cctcttcgtc gtcgtcgaaa
     1501 tagttacctg tttcggctga aaggtataaa tcccctctac ctgtaataat accttcagaa
     1561 ttttcaagtc gccaggggca ttggatgtgc aatgcaaagt cacctacagt acctttcttg
     1621 tccttccttt tttttctact aagacggcga ccctcaactt ctcggatggt accgaaatgc
     1681 agaaccaaca tatttgccgc acgacgagca atggagagtg ggagacctac taattgatcg
     1741 agatgttttt tgatttctgc tttcgttgat gacatactat tgattttttt ctcttgaatc
     1801 gtagaacacg ctttgagtta catatacatc attcgagtgt gttgttgcaa cacttgtcat
     1861 tacggcgctg agggccagcg gactgtgatg acgagcgatt cttcctcgca acgaaacgag
     1921 tgcggcacgc cgggaccaaa gataacaaag tctccctcct tctcaagagg gacttcctga
     1981 tctggaaaca gcaggacaaa gtggcctcgg ataaggatat tgagcgtccg aacgggattc
     2041 ccgggtgccc attcatgacg agtctctccc ttggcgtggg tataccactt caactccact
     2101 tcctcggtat gaagtggatt ctcttcaccc ggcatgaagt gaccgagaaa ccagccacga
     2161 gtgtcctttg aaacgtcact gacgttccct gaaaacggag cagatgtatt tgagttcatg
     2221 tttttctcct ggtgatttat gactggccag catgtgcatg ctgtattttg ctgcaatctg
     2281 acaggcatcg gttcatgtta cagactactg gctaagattg tgccaagacg aactgttttt
     2341 tctaaccatt catttcgtat gccaatcgga acccaccaaa gatgtactcc gtcgctattt
     2401 atcaaaatta ccggtccgac aagctgtatt ttggtccagg tgacaattct aactggagtg
     2461 aagttatgag cttgtgcggc aacacttcac gttctgtgcg caacaggaac aattctcgaa
     2521 attcatcagt tccgatggat ttctctggca ggtcagatag catattttcc atgtttgcct
     2581 tgtcagcagg atttttgaaa ctgtgttcat tctccagagc aaggcgtgga tggacaatct
     2641 gctttagttt atctcttcgt gggtaaatca atatcgggtg ggtgggcaaa accacgcttt
     2701 tcgttgtcca cgcgattact tgaatatagg gtgtagaccc tggtggtaac tttcggtaat
     2761 gatcgtaatt gtaatattcc atttcccact gaataccatt ttgctgtcct ctgaacaaat
     2821 gatctcgatg atctttagga tgcggttgaa ccgtccatcc atttctttct gacaacgcct
     2881 taaagcgcgg agataatatc gccacgatta agaaagaaaa tataaatgga ctacataagg
     2941 cgagagagac gatcaggata tttatgatgt tggcatccat agatgtgatg gcaagttaga
     3001 aggcaaagtt tccgaaatcg gctgctcacc gtaacatttt ctcccaaact aataattttc
     3061 gtcttttatc agtttgacaa aaattccttt gcgtcgtttt tcaacgacga tcctgctcat
     3121 tatttcctct ctaagatgtt ttggaagatg tcgcgcttgc caatcgcgtt cttccgcaac
     3181 gtttctttct gcattagcgg ggtcttttgc tgcgatggct tttaacgcgt agagggcgcc
     3241 gccatagcca tgctgggcga cgtgagccgt tgccactgcc tgacctgctg cccgcgcggc
     3301 aaagagcgca gggtcattgt ctttcacatc acgtgcggcg gcgtgagagc tgagagaggc
     3361 accgcgtatt tcagccatct tgaacacacc tgtccgaacc caggtacggc aggtttcgag
     3421 tgctttccgt ggacggtcat cctcgggata ggcttcctca aaaaacggca ggacgcgttc
     3481 ggcacagtcc gctgcccagg ttgccatgag tttctgatct tctttactgt attttttcat
     3541 gatcaccgca cgcgtttata ccggctgtag ccactcagag aaagctcgcg aggcatcaag
     3601 acatagacgg tttccgcggg aacggtcagt gccaggaagc ccgcattgat catccctaca
     3661 ggcttggcat ctgccggacc cagcgcatcc accatcggca agtagtccgt caga
//
LOCUS       DASTJP010000175        15328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_761701, whole genome shotgun sequence.
ACCESSION   DASTJP010000175 DASTJP010000000
VERSION     DASTJP010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15328
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761701"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFQ23_05285"
     CDS             <1..668
                     /locus_tag="VFQ23_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9906026.1"
                     /translation="IGLFAPLGLALLMNNPNVKGQFVLRSLFYMPFIVPFVASVFLWG
                     GMLNSETGWINRVLVALGTPKDSLPLWANDVNWVYPTYVIMGIWGIGNAMLIMLAGLQ
                     GVPTELYDAAKVDGANSWATFWNITFPMISPVIFYNLVLNMVGLFQYFLVPLVVNNGT
                     GRPGGATLFYNLYLYKTFFTFQNMSYGSTLAWFLFLIILAVTIFLFATAKYWVYYAGE
                     ARS"
     gene            680..1744
                     /locus_tag="VFQ23_05290"
     CDS             680..1744
                     /locus_tag="VFQ23_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9906027.1"
                     /translation="MNKSVRKGISATFFTALAIGLLGVFLSPFAFMIFTSLKTQDQIS
                     IVGAPIWPAQPSFYEHEGKKLEVFTVPLAKCAGYDASSSDTANLALFKKGRQESTFMD
                     PSDPGKEPFLCQVSWRALDRPWQFSPTWENYARVWESIPNGYLRLLANTTFYAVITMI
                     GTVISCILVAFGFARFRFPGRDFLFLILISTIFLPATVTLIPTYSFWQRLGLVGTWIP
                     LVLPHFFANAYNVFLFRQYFLTLPRELDESAMMDGASPLRILWSIIIPQSYPVIIAVV
                     VFHIVFAWNDYFGPLIYLSTARENWPISVALSSFNGIYGQQPQLIQAGALMALILPVI
                     LFFVAQRFFVQGIVVTGVEK"
     gene            1746..3044
                     /locus_tag="VFQ23_05295"
     CDS             1746..3044
                     /locus_tag="VFQ23_05295"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET9906028.1"
                     /translation="MLKVAVIGGGSTYTPELVNGFLARVDKFPLDELWLVDVDEERLR
                     VVGGFAQRMVEAKGAPFKVILTSNQREAVKDASYVTTQLRVGHMEARRRDEYLGLRHG
                     LIGQETTGIGGMAKALRTIPVILKIAQAIREVAQPGTMLVNFTNPAGLVTQALSKYAA
                     DVPAVGVCNVPITTKMHILEGLEKSTGKKIEPDHAELKTLGLNHLSWHRGFTIDGEDV
                     WPQVIEGFINELKAEDHPEWEPRTIEVLRMMPNYYLQYFYHTDKKLKSQEAWPPSRAE
                     EVIEIEKDLLREYADPNLNEPPADLMKRGGAYYSTVATQLLNAHYNDLGETHVVNIKN
                     DGAVQEWPADWVLEIPSTVKKTGITPIPTEPLPASCFGLIAAVKAYELLTVEAAVHGD
                     REAAYQALLVHPLGPKADKVQAVLDDMLETHKEHLPQFWN"
     gene            3108..4103
                     /locus_tag="VFQ23_05300"
     CDS             3108..4103
                     /locus_tag="VFQ23_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET9906029.1"
                     /translation="MRYFLGTDIGATKTHTLIVNETGRALGFGESGPANHETVGYEGM
                     FQSMHSGLEQALRGAGLNKADITGAGFGVAGFDWPSEYADTDTTISRLGLNAKYQFVN
                     DAVPGLIVGSDEGWGVVVVSGTGSNCRGWDRERKREGRVTGHGVLMGEGAGGTELMHR
                     CMQLVGYAWSKRGPKTKLADAFIEHVGAKDLEDLIHGYTVGAFSLGAEVAPIVFRVAE
                     QGDQVARELIHWAGCELGEMANAVIRQLEFENFAFDVVMTGSMFEGGELLIVPMRETI
                     HKFAPKARLVRLTAPPVLGAVMLGMEASQFDVTPAIRKTLIDSVSLFRNIPVRQS"
     gene            4100..5305
                     /locus_tag="VFQ23_05305"
     CDS             4100..5305
                     /locus_tag="VFQ23_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9906030.1"
                     /translation="MTTIDFSLIPDADGHYESIQRLMSSFRRETGIDVNLKRMEWENA
                     WPQLISISTQGQGADISHVGSTWVSSLMTMNALRPIPAQLISKVGGDQAFVHSTWSNV
                     VAEEDRFAYGLPFSAYVYIVAYRKDLLKKAGLNGSTAFATPHALEESVQRLGSLNETE
                     VPWLMPIVPHPFNDLVHMAASWIWSSGGHIMDNRGKQILINSPAALAGLKSFLRLLRN
                     VPNDGYLGADMCMEALLDGKAAAVVTDVASLLNALQNQSPKIENIGAASLMSIPWSGG
                     GSIVIWRHTYGYPDRLEASYKLAEFLVRKHSMLELANQSNILPARTDALDELFPPNHI
                     LRPVMLQLISTGRSYRPIALWHRIEHQFGVELGEAANTLLKDPDADLDTVVTQTMNSL
                     ADRLNLTLE"
     gene            5307..6299
                     /locus_tag="VFQ23_05310"
     CDS             5307..6299
                     /locus_tag="VFQ23_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HET9906031.1"
                     /translation="MRHLLTVLSLLAVLLASCSPQATPINTAEATVTPAPMMTPTSVP
                     PSFIILAYATDGIIPELIPYEQLTHINYSFLTPKEDGTFNRINNSWKLKQIVETAHEH
                     DVRVNIAVGGWGWDAQFEELAASSETRSAFVQNLTAFVDEYQLDGVDIDWEYPDAGQS
                     SQNFLALVKELRTAMPDKLLTTAVVSYGDDNGIGIASESFEFFDFVNVMTYDGPDHGS
                     MEQFEKGISYWSARGLPKEKIVMGVPFYSHVKDSRAEGVTYARLVSDDPAAAQADEFD
                     YSGMIQIYNGIPTIQAKTNIAMQQANGIMFWALDHDTQGDLSLVNAIYQAAYQP"
     gene            6385..7611
                     /locus_tag="VFQ23_05315"
     CDS             6385..7611
                     /locus_tag="VFQ23_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9906032.1"
                     /translation="MTDFTLGVNYWPRHKAMYWWSNFDSGEVREEFAILKEIGLNVVR
                     IFLLWDDFQPDPTSVSEAMVKNLVTVADIAAETGLGIDLTFFTGHMSGPNWSPRWLLG
                     GELPPAAHQHWLRDVVSEGKRVDTGYRNMFHDEMALEAERLLVRTVVSALKDHPGIWM
                     WNLGNEPDLFAWPNSSEEGAAWVREMVELIKEIDPNHPVTIGLHGDGLHRDNGLRIDK
                     VYAHTDVAVMHSYPMYTPWARKPLDPDFVPFTCAMTAALAGKPVLMEEFGGCTALPGE
                     ASYVMKWTETSGREREQFMASEEDFAEFLRLTIPKLQHSGATGAMVWCFADYVPELWD
                     RPPCQNAVHERFFGLVRPDGSLKPHAKVIQEFAATKPQVQPIPEYARFEVNPDEFYKN
                     PTPFLLDFYAQYLKGV"
     gene            7663..8460
                     /locus_tag="VFQ23_05320"
     CDS             7663..8460
                     /locus_tag="VFQ23_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HET9906033.1"
                     /translation="MKQLIINSDDYGRTPDISRGIRESHLNGVVTSTTCMMNIPTTAA
                     DVAIALKETPALGLGVHLVLTMGRPITGGAGSSITDENGNFFKYVPFVENVPQMDMGE
                     VKAEWRAQIESFIKAAGRKPTHLDSHHHSSYFAPKLFRGMLELAKEYDCAIRFPFTHT
                     ISSELEETHKHVPQLIEEFNPRRPDAFLVDFYDQNATSEELLRLFNHVEDGTTEIMCH
                     PGYVDDAFAQESTYNFQRERELKILTDPEIKKVIDATGIKLATFADL"
     gene            8541..11183
                     /locus_tag="VFQ23_05325"
     CDS             8541..11183
                     /locus_tag="VFQ23_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET9906034.1"
                     /translation="MSRIEELLEQMTIEEKISMLAGTELWYSTAVKRLGIPAFRMADG
                     PNGVRGIWSEMSPTAAATPVGMALGATWNPDIVEKVGNVLGEELKDKCGHILLAPTVN
                     IHRTPIAGRNFECFSEDPYLSGMLASAYIQGVQSQGVGACIKHFVANDQEYERQSISS
                     EVDERTLREIYLEPFRIAIRNSNPWAVMSAYNRVNGRYACEYDHTLLEILKGEWGYEG
                     IVISDWFGTYDHEVPHGGLDIEMPGSARWMSEEHVKRALDDGPLTEERLDDKVKRLLS
                     VLQKAGLFDNEEIHPERANDNPRHRQILREAAGEAIVLLKNDDDILPLKKVKSIAVIG
                     PHAAHASIFGGGSSSGFRPHYVISPLEGIKTRAGNKIQVDYTPGCLIHKTLPAPAHET
                     LSTADGRRGLNLSLFNGTNFSGDPVFTDVTPFVDHGWFHVSVPNANQESFSMIQEGFF
                     TPNESGVHKLALGGVGWSKLFFDGQLLIDHSHDSDMGREFIAEIELKGGKAYPIRVEY
                     YWQGDPRWRSVKLGHLPPQSEDPIEDAVKLAKKADVVVLVAGLNSEWESEGFDRVDMK
                     LPGLQDELIKRVAKANKNTIVVVNAGSAIEMPWIDKVSAVLQLWYNGQEQGNALADVL
                     FGDVNPSGKLPTTLPVRLEDNPAYINYPGENGKVRYGEGIFVGYRYYDKKKLAPEFPF
                     GHGLSYTDFKYSNLRLSEDQTTLEQGLDVSLDVTNKGKRAGKEIVQLYVRDIKSSLTR
                     PEKELKAFAKVELAPRETKTINFHLDREAFWFFDPAENGWVIEPGEMEILIGASSQDI
                     RLRDMVTLLAGRESPLHVGLPLRILLENEAGHAVLARYFGDILETPAVQMAREMSLKQ
                     ISEISPNVLPPETLQAISDDLAKA"
     gene            11202..12269
                     /locus_tag="VFQ23_05330"
     CDS             11202..12269
                     /locus_tag="VFQ23_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9906035.1"
                     /translation="MKSKLITLWIVFSILLAACGAEAVAEVTPTPFPTPVRQTFTVQR
                     GDFTVEAKLSGRVSPLALHTVYFQIDGQVSEVLANVNDVVTEGQLLGELAEARELRAQ
                     AEETRRVIRRAQINLEIAQLTLEQFQAQGRPEYDIRVQELQVELAQMELDEVLTSLGI
                     DPDSEALDELDAQVAQARAFAPADGTIIASVNVGRNVNPSTPAFVLGDPDELEIVAEL
                     DASKGDEEVREMFEGMPVVVTLDAKPDTELTGTIRQLPSPYGTGEADERVIHIVMDSP
                     PSASTYQSGDKVTVTVLLASKQGALWLPPEAIRQAGGRTFVIINTESGPSRVDIEIGL
                     ITRDMVEILSGLTEGQVVLGS"
     gene            12266..15178
                     /locus_tag="VFQ23_05335"
     CDS             12266..15178
                     /locus_tag="VFQ23_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9906036.1"
                     /translation="MSLFSGLSSFALQFWATAGIAFKRLLTQRFLSLASIAGLMIASG
                     FILSVPLYADATYFRLLREELLAGRESELTQKPVDYAPLSFIFELKAAGRGSPQWKNV
                     VDVDTYLSSTALRTIDLPILQVVRRFRTDGYNMYAPRDPSNTGTQYPLTTANLVFMTP
                     LEENIIILNGRTPQPFVSLLREGDAIEAMASENLADEFGVQTGDIYTLRTDNGPLPIT
                     IVGLWRPADPLAPYWDVKRDTWLFVHEETYKSIISDTVDDELISSTWSIVADGSSLHA
                     NDVDGLKERILAIESRAATLLPKTALIASPLDALERYQANAPTLTYLLFAFSVPILSL
                     ILAFIGLVAGLFVGQQRGEMAILRSRGASAFQVVSMSLMQGVLLAIVALAGGIALAYW
                     ITHSIGKARSFLDFSAVGGLRVDLTPQVLGYGLLGIAIILLIQVLLPTLSAAENTIVT
                     YKQERARSFRNPWWQKYWLDILLLIPAGYGLWQLTQQSKEALGGSETIPDPLQNPLLL
                     LVPALGIFSVALFTLRLVPRLMDFVSWALHTSKSVGLLMASRYLARTPAFYSAPLVLL
                     VLTLGLSAFTASLARTLDSQLNKQMRYQVGADINIYEFGTTFNEDSPNPVYSFVPVED
                     HLFIPEVRSATRVGRYKFSAIGQSGSIQGAFLGIDRLTFPSAAYWQRDFASEQLGVLM
                     NQLAADPNGILVPTSLLEQQNLELGDTLKLAITTGVAGESIPLEAHIVGSFDLFPTWY
                     PETGTMIVGNLEEMFLRAGAEYPHEVWLGMNRSADPELIVYAVRGFSILLDPQADQTK
                     LVQNGLNTIVREWSSAELKIRDEQRRPERQGLFGLLSVGFIASALLTVLGFLLYALFS
                     FRRRFIEMGMLRAIGLSIKQMTSLLAAELAFLVLLGIGVGTALGVFASRLFVPFLQIG
                     ASAQAQYPPFQIEIAWLSIVEIYVLFVILFIVALSALSALLVRMKIFQAIKLGETT"
BASE COUNT         3645 a         3912 c         3974 g         3797 t
ORIGIN      
        1 caattggact ttttgctcca ttgggattgg ctcttctgat gaacaacccc aacgtcaaag
       61 gacaatttgt tcttcgcagc ttgttctaca tgccttttat cgtgcccttt gtggcgagcg
      121 tcttcctttg gggaggcatg ctcaactccg agaccggctg gatcaataga gttctagtag
      181 cattaggaac ccccaaagac agtttgccgc tctgggcaaa tgacgtaaat tgggtatatc
      241 cgacgtatgt catcatgggc atttggggca tcgggaatgc catgctgatt atgctggccg
      301 gtttgcaggg tgttcctaca gagctttatg atgcagccaa agtggatggc gccaacagtt
      361 gggcgacctt ctggaacatc actttcccca tgatttcgcc ggtcattttc tataacctgg
      421 tactgaacat ggtcggttta tttcagtact tccttgtacc tttggtggtg aataacggga
      481 cagggcggcc gggtggtgca acgctgttct acaatttgta tctttataaa acatttttta
      541 ccttccagaa catgtcgtat gggtcgactc tggcctggtt cttgttcctt attatcctgg
      601 cggtaacgat ctttttgttc gccactgcca aatattgggt ttactacgcg ggcgaagccc
      661 gctcgtaaag gaggcatcca tgaacaaatc tgttcgcaaa gggatcagcg caactttctt
      721 tacggcttta gcaatcggct tgctgggagt ctttctctct ccgtttgcct ttatgatttt
      781 cacgtcactc aaaacgcagg atcaaatctc catcgtgggt gcccccatct ggcccgcgca
      841 gccttcgttt tatgaacacg aaggcaagaa actcgaagtt ttcacggtcc cgctggccaa
      901 atgtgcaggc tatgacgcca gcagtagtga taccgccaac ctcgcgctct ttaagaaggg
      961 ccgtcaggaa agcaccttca tggatccttc cgacccgggc aaggaaccgt tcctctgcca
     1021 ggtctcatgg cgcgcgcttg atcggccgtg gcaattttct ccgacatggg aaaactatgc
     1081 cagagtttgg gaaagcattc caaatggata tctgcggctg cttgccaata ctacgtttta
     1141 tgcggtcatt acaatgatcg ggaccgtaat ctcatgcatt ttggtggcgt ttggttttgc
     1201 ccggttccgc ttccctggcc gtgatttcct ttttctcatc ctcatctcca cgatctttct
     1261 tccggccacc gttactctca tccccacgta ttccttctgg cagcggcttg ggctagtcgg
     1321 cacgtggata cctctcgtct tgcctcattt ctttgccaat gcttataacg tcttcctgtt
     1381 ccgacaatac ttcctaacat tgcctcgcga actggatgaa tcagccatga tggatggcgc
     1441 cagcccgctg cggattctct ggtcaatcat cattccccaa tcctaccctg tgatcatcgc
     1501 cgtggtcgtt ttccacatcg tctttgcatg gaatgattat tttggtccgc tcatttacct
     1561 ctccacagca agagagaatt ggcccatctc ggttgcactt tcatcgttca atggaattta
     1621 tggtcaacaa ccccaactga ttcaggcggg cgcattgatg gcgctcatcc tgcctgtcat
     1681 tttgtttttc gttgcccagc gtttctttgt acagggaatt gtcgttacag gcgtcgaaaa
     1741 ataatatgct gaaagttgca gtgatcggtg gaggctcgac atatacgcct gagcttgtca
     1801 acggctttct cgcacgtgtg gataaatttc cactcgatga gttgtggctg gtggatgttg
     1861 atgaagagcg ccttagagta gttgggggat ttgcgcaacg catggtcgag gcaaaaggcg
     1921 ccccattcaa agtcattctt acatccaatc agcgtgaggc ggtcaaagat gcttcttatg
     1981 tgacgactca actacgcgtc gggcacatgg aagcgcgccg ccgggatgag tacctcgggc
     2041 tgcgtcatgg cttgatcggc caggaaacga caggcattgg tgggatggca aaagctttgc
     2101 ggaccattcc ggtgattttg aaaattgcac aggccattcg agaagtagct cagcctggca
     2161 cgatgcttgt caattttaca aaccctgctg gtcttgtcac acaggccttg agcaaatacg
     2221 cggcagatgt tcccgcagtg ggtgtctgta acgttcccat cacaaccaag atgcacattc
     2281 tcgagggtct ggaaaaatct accggaaaaa agatcgagcc cgatcatgca gagctaaaaa
     2341 cccttggact gaatcacctg tcctggcatc gcggcttcac aattgacgga gaggatgttt
     2401 ggccacaggt gattgaagga tttatcaatg aactaaaggc tgaggatcat ccggaatggg
     2461 agccgcgcac gattgaagtg ctgcgcatga tgccgaatta ttacctccaa tatttctatc
     2521 acaccgacaa gaagttgaaa tcgcaggaag cctggccacc gtctcgcgcg gaggaagtga
     2581 tcgagattga aaaggatttg ctgcgcgagt atgcggatcc gaatctaaac gaacctcccg
     2641 ctgacttaat gaagcgcggt ggagcttact actcgactgt tgcgactcag cttttgaacg
     2701 ctcactacaa tgatctgggc gagactcatg ttgtaaacat aaaaaatgat ggagcggtgc
     2761 aggaatggcc agcagattgg gtgttggaaa ttccttctac cgtgaagaag actggcatca
     2821 cgcctattcc gaccgaaccg ctgcccgcct cctgtttcgg gttgatcgcg gcagtaaaag
     2881 cctatgagtt actgaccgtc gaagcggcag ttcatggaga ccgagaagcg gcgtatcagg
     2941 ctctgctggt tcacccgctt gggccgaaag cagataaggt tcaggcagtg ctcgacgata
     3001 tgcttgaaac gcataaagag cacctgcccc agttttggaa ctgacctcag tcaggggcga
     3061 cccaccaggt cgcccctgta ttatagatat atagataaca aacagatatg cgctactttc
     3121 tcggcaccga tatcggtgcg accaagaccc acaccctcat cgtcaatgaa actggccgcg
     3181 cgcttggctt tggtgaaagc ggacctgcta accatgaaac ggttggctac gagggaatgt
     3241 ttcagtccat gcatagcgga ttggagcagg ccttgcgcgg tgcaggcttg aacaaagccg
     3301 atatcacggg agctggcttc ggcgtagcag gattcgattg gccttccgaa tatgcggata
     3361 cagacaccac catcagtcgt ctcggtctta atgcaaagta tcaattcgta aacgacgcag
     3421 taccgggcct catcgtcggc tcggacgaag gttggggcgt cgttgtggtt tcaggaaccg
     3481 ggtcaaactg ccgcggttgg gatcgtgaac gcaaacgtga aggacgcgtg acgggtcatg
     3541 gagtgctgat gggtgaagga gccggcggca cggagctcat gcaccgctgc atgcaattgg
     3601 tcggttatgc ctggagtaaa cgcggtccca agacaaaact ggcagacgca tttatcgaac
     3661 atgtgggggc gaaggatttg gaagatttga tccacggtta tacagtcggt gctttttcac
     3721 tgggtgcaga ggtcgcgcca attgtctttc gcgttgcgga acaaggagat caggttgcgc
     3781 gtgagctgat tcactgggca ggctgtgaac tcggtgaaat ggcaaatgca gtgattcgcc
     3841 aactggaatt cgaaaatttt gcatttgatg ttgtgatgac cggcagcatg tttgaaggtg
     3901 gagagttatt gattgtaccc atgcgggaaa cgattcataa gtttgctcca aaggcccgcc
     3961 tggtacgcct cactgcccct ccagttttag gtgccgtgat gttaggcatg gaagcaagcc
     4021 aatttgatgt gacgcccgcc atccgtaaaa cgttgatcga tagtgtctct ttgttccgga
     4081 atattccagt aaggcagtca tgaccacgat tgatttttcg ctgattccag atgcagatgg
     4141 tcattacgaa tcaatccagc gattaatgtc ctccttcagg cgtgagacag ggatagacgt
     4201 gaatctcaag cgcatggagt gggagaatgc ctggccacaa ctcatcagca tttcgacaca
     4261 gggacagggt gcagatattt ctcatgtcgg ttccacatgg gtaagcagcc tcatgaccat
     4321 gaatgcgttg cgtccgattc cggcacaatt gatcagcaag gtcggtggtg atcaggcctt
     4381 tgtccattcc acctggtcga acgtcgttgc agaggaagac cgtttcgcct atgggcttcc
     4441 attcagcgct tatgtgtaca tcgtagccta tcgaaaagat ctgctgaaaa aagccgggct
     4501 aaatggttcc acggcatttg ctacgccaca cgccctcgaa gaaagcgttc aacgtcttgg
     4561 ctcgctcaat gaaacagaag tcccatggct gatgccgatc gtgccacatc ccttcaatga
     4621 tctggtccat atggccgcct cgtggatttg gagttctggc gggcacatta tggacaatcg
     4681 tggcaaacaa attctgatta attctccggc tgcactggcc ggattgaaat catttttgcg
     4741 tcttttgcga aacgtgccga acgacggcta tctgggcgcg gatatgtgca tggaagccct
     4801 cctcgatggg aaagctgcag ctgtcgtgac cgatgtggct tccctgctca atgcgcttca
     4861 aaaccaatct ccgaaaatcg aaaacattgg tgcggcaagc ctgatgagca ttccctggtc
     4921 tggcggcggg agtatcgtga tatggcgaca cacctatggc tacccggacc gcctcgaagc
     4981 ctcgtataaa ctggctgagt ttcttgttcg caaacattcg atgcttgaac tggcaaatca
     5041 atcgaacatc ctgccggcgc gcaccgacgc attggatgaa cttttcccgc ccaatcatat
     5101 tttgcggccg gtaatgcttc aactcatctc aaccggacgt tcctatcgcc ccattgctct
     5161 ctggcatcga attgaacatc aattcggcgt agagcttggc gaggcggcaa atacgctttt
     5221 aaaggatcca gacgccgacc tggatactgt tgtaacgcaa acgatgaact cccttgcgga
     5281 ccgtctgaat ttaacacttg aataaaatgc gacatctctt aacggtcctg agcctgctcg
     5341 ccgttttgct ggcttcctgt tcccctcagg cgactcccat caacactgcc gaagccacag
     5401 taacacctgc acccatgatg actccgacgt cagtccctcc ttcctttatt attctcgcct
     5461 acgccaccga tggaatcatt ccagaactca ttccttatga gcaactcact catattaatt
     5521 attccttcct tacaccaaaa gaggatggga cgttcaaccg catcaacaac agctggaagc
     5581 taaagcaaat tgtcgaaacc gctcacgaac atgatgtgcg tgtgaatatt gctgttgggg
     5641 gttggggctg ggatgctcaa tttgaagagt tggccgccag ttctgaaact cgatccgcat
     5701 ttgtccaaaa cctgactgcg ttcgtggatg aatatcaact cgatggagtg gacattgact
     5761 gggagtatcc ggacgccggg caatcctccc aaaatttcct ggcactcgtt aaagagttgc
     5821 gcactgcgat gcccgataaa cttctgacaa cggcagtagt ctcgtatggg gatgataatg
     5881 gcattggcat cgcgtccgag tcatttgaat ttttcgattt cgtcaacgtc atgacctacg
     5941 acggtcccga ccatggatcg atggaacaat tcgagaaggg aatttcctat tggagcgcac
     6001 gcggcttgcc caaggaaaag atcgtaatgg gtgtgccatt ttattcgcat gtgaaagact
     6061 cacgtgccga gggcgtgacc tatgcgcggc tggttagcga tgaccccgcc gcagcgcagg
     6121 cagatgaatt cgattattca gggatgatcc aaatctataa cggcatccca accattcagg
     6181 caaaaacaaa cattgccatg cagcaagcca atggcattat gttctgggca ttggatcacg
     6241 atactcaagg tgacctctcg ctggtcaacg cgatttatca ggcagcctac caaccataaa
     6301 catattcgcg ccgagtaatt cgcaacgaag cgcggttgca aatgcaatac ctgcttttac
     6361 tacactcttc atccggagac cttgatgact gatttcaccc ttggtgtcaa ttactggccc
     6421 cgccacaaag ccatgtattg gtggagcaac tttgattccg gagaagttcg tgaggaattt
     6481 gcaatactca aagagatcgg actaaacgtc gttcgaatat ttttattgtg ggatgacttt
     6541 caacccgacc cgacaagtgt ttcagaggct atggttaaga acctggttac ggtagcagat
     6601 atcgctgccg agaccggact tggcatcgac ttgactttct tcaccggaca tatgagcgga
     6661 cccaattgga gtccacgctg gttactgggt ggagagctac cacctgcggc tcatcaacat
     6721 tggctgcgtg atgttgtcag cgaaggaaag agagttgata caggctatcg aaacatgttc
     6781 cacgatgaga tggcgcttga agccgagcgt ctgttagtaa ggacagttgt gagcgcactg
     6841 aaagatcatc caggaatctg gatgtggaac ctgggcaacg agccagatct attcgcctgg
     6901 ccgaattcat cggaggaggg cgccgcctgg gtcagggaaa tggtcgaatt aatcaaagaa
     6961 atagatccga atcatccggt cacaatcggt ttacatggcg atggcttaca tcgggacaat
     7021 ggcttgagaa ttgataaggt ctacgcgcat actgatgttg ccgtgatgca ttcctacccc
     7081 atgtacacgc cctgggcacg caaacccctt gacccagact ttgtcccttt tacatgcgcg
     7141 atgacagccg cgctggctgg aaagcctgtg ctgatggaag aatttggtgg atgcactgcc
     7201 cttcctggtg aagcatccta cgtgatgaaa tggacagaga caagcggacg tgagcgtgaa
     7261 cagttcatgg ccagcgagga agactttgca gaatttctgc gactgaccat tccaaaactt
     7321 cagcatagtg gtgcgaccgg cgcgatggtc tggtgctttg cagactatgt gccagaatta
     7381 tgggaccgtc caccctgtca gaacgccgtc catgaaagat tctttgggct ggttcggcca
     7441 gatggctcgc tcaaaccgca tgccaaggta attcaagagt ttgccgcgac aaaaccgcag
     7501 gtacagccca tcccggagta tgccaggttc gaggtaaacc cggatgagtt ttacaaaaac
     7561 ccgacgccct tcttactcga cttttatgca caatatttga aaggtgtcta aatttgctct
     7621 tgccgccggg gacggcggcg aggacgccgc ctggagcata ctatgaaaca actaatcatc
     7681 aactcagacg attacggccg cacgccagat atctcacgtg gcatccgcga atcccatttg
     7741 aatggtgtgg tcacatccac cacctgcatg atgaacattc caacgactgc cgccgatgtt
     7801 gccattgcct tgaaggaaac accagctctg gggctgggag ttcatctggt tctgacgatg
     7861 ggccggccga tcacaggtgg agctggctct tcgatcactg atgaaaatgg aaatttcttc
     7921 aaatatgtgc cttttgttga aaatgtccca caaatggata tgggcgaagt caaagcagaa
     7981 tggcgtgcgc agatcgagtc cttcatcaaa gcagcaggtc gtaaaccaac tcatcttgat
     8041 tcgcatcatc actcctccta tttcgctccg aagcttttcc gcgggatgct tgaattggca
     8101 aaggaatatg actgcgcaat ccgctttccg tttacacata cgatctcttc tgaattggaa
     8161 gagacccaca aacatgtgcc gcaattgatc gaggaattta acccgcgccg accggatgcc
     8221 tttctggtgg atttctatga ccagaacgca acgagcgaag aattactgcg cctcttcaac
     8281 cacgtagagg acggtaccac tgaaatcatg tgccatcccg gttatgtcga tgacgccttt
     8341 gcacaggaat ccacttataa ctttcaacgc gagcgcgaat taaagatcct gacagacccg
     8401 gaaatcaaaa aagtgattga tgcaactggc atcaaattag ctacgttcgc tgacctttag
     8461 tcccctctcc catagggaga gggctcgggt gatggagaat tctttcctca tcctccctac
     8521 attagcacct ggagtatttc atgtctcgaa ttgaagaact gcttgaacaa atgaccatcg
     8581 aagaaaagat ttccatgctg gccggcaccg aactctggta tagcacggct gtgaaacgcc
     8641 tgggcattcc agcgttccgt atggcagatg gacctaacgg tgtccgcggc atctggagcg
     8701 agatgtcccc cactgctgcg gcgaccccgg ttggtatggc gctcggcgca acctggaatc
     8761 ctgacattgt ggaaaaagtt gggaatgtcc tcggtgaaga actgaaagat aaatgcgggc
     8821 acatcctgct tgcgccaacg gtaaatattc accgcactcc gattgccggt cgcaactttg
     8881 agtgcttttc tgaggatccc tatttgagcg gcatgcttgc ctcggcttat atccaaggcg
     8941 ttcagagcca gggcgtgggg gcctgcatca agcatttcgt tgccaacgat caggagtacg
     9001 agcgacagtc catcagctca gaagtagacg agcgcacctt gcgagagatc tatctggagc
     9061 cattccgaat cgcgatccgc aattcaaatc cgtgggcggt catgtccgcc tacaaccgtg
     9121 tcaacggacg ctatgcctgc gaatatgacc acacgttgct ggagatcctc aaaggcgagt
     9181 ggggctacga aggcatcgtc atctccgact ggttcggcac gtacgatcac gaagtaccgc
     9241 atggtgggct tgatatcgaa atgccgggct cggcgcgttg gatgtcggaa gaacacgtca
     9301 agcgcgcgct ggatgatggt cccttgactg aagagagact tgacgacaag gttaagcgat
     9361 tgctcagcgt gctccagaag gcgggcctgt ttgataacga agagattcac cctgaacgcg
     9421 ccaatgacaa cccgagacat cgtcagatcc ttcgtgaagc agcaggtgaa gcgattgtcc
     9481 tcctcaaaaa cgatgatgat atcctgcctc ttaagaaagt caaatcgatt gccgtgattg
     9541 gtccgcatgc cgcgcatgca tccatctttg gcggcggcag ttcatccgga ttccgcccac
     9601 attatgtaat ctcaccgttg gagggaatta aaacccgcgc cggaaataag attcaggtgg
     9661 actacactcc cggctgcctc atccacaaaa ctctgcctgc tccggctcat gaaacgttat
     9721 ctactgcgga tgggcgccgt ggactcaacc tcagcctgtt caatggcacg aatttttcgg
     9781 gtgaccccgt cttcaccgat gtgaccccgt ttgtcgatca cggatggttc cacgtttcgg
     9841 tgccaaatgc gaatcaggaa tcgttctcga tgattcaaga aggattcttc acaccgaatg
     9901 aatccggagt ccacaagctg gcgcttggtg gcgtcggctg gagcaaatta ttttttgacg
     9961 gccaattgct aatcgatcac tctcacgatt cagacatggg gcgtgaattc atcgcggaaa
    10021 tcgaacttaa gggcggcaaa gcctacccga ttcgggttga atactattgg cagggtgatc
    10081 cgcgctggcg ttccgtcaag ctggggcact tacctccgca atccgaagat cccattgagg
    10141 atgctgtcaa gctcgcaaag aaggcagatg tagtggtgct cgtggcaggc ttgaacagcg
    10201 aatgggaatc cgagggcttc gatcgagtcg acatgaaatt gcccggtctg caggatgaat
    10261 tgatcaaacg tgtggcaaag gcgaataaga acacgattgt cgtcgtgaac gcaggttccg
    10321 ctattgaaat gccgtggatt gataaagtat cggctgtgct gcagctctgg tacaacggac
    10381 aggaacaggg caacgcgctg gcagatgtgt tattcggtga tgtcaacccc tcgggcaaat
    10441 tgccgactac gctcccagtc cgtctggaag ataatcctgc ctatatcaac taccctggcg
    10501 aaaatggaaa agttcgatat ggtgaaggta tcttcgttgg ctatcgctac tacgacaaaa
    10561 agaaactcgc gcctgaattc ccatttgggc acggcctttc atacacagat tttaaatata
    10621 gcaatctgcg gctgagtgaa gatcaaacca cacttgaaca gggattggat gtcagccttg
    10681 acgtgaccaa taagggaaaa cgtgcaggca aggaaattgt ccagctttat gtacgagaca
    10741 taaaatcctc gcttacaaga cccgagaagg aactgaaggc atttgcaaaa gtggagcttg
    10801 cgccgcgtga gacgaagaca attaattttc atctcgaccg cgaggcgttc tggttctttg
    10861 atccggcaga gaacggctgg gtaatcgaac ccggtgaaat ggagattctg atcggggcct
    10921 ccagccagga catccgcctg cgtgacatgg tcacgctcct ggccggtcgg gaaagtccac
    10981 ttcatgtcgg cttgcccctg cgcatcctgc ttgaaaacga agctggtcat gcagttttag
    11041 cgcgctactt tggcgacatc ctggaaactc ctgccgttca gatggcaagg gaaatgtcgc
    11101 tgaagcaaat ctctgaaatt tcgccaaacg tattgccgcc tgaaacctta caggcaatta
    11161 gtgacgacct ggcaaaagca taaacagatt gtatttggat aatgaaaagt aaactaatta
    11221 ccttatggat tgtattctca attttgctcg ctgcatgcgg tgcagaggcg gtggcggaag
    11281 tcactcctac tcccttcccc acacccgtac ggcagacatt cactgtgcaa cgtggtgatt
    11341 tcacagtaga agcaaaacta agcggacggg tctcacctct tgcgttgcat accgtctact
    11401 tccaaatcga tgggcaagtc agcgaggtgc ttgcgaatgt caatgatgtg gtcaccgagg
    11461 gtcaactgct cggtgaattg gcagaagcgc gagaattgcg agcccaggca gaggagactc
    11521 gccgcgtcat tcggcgggcg cagatcaacc tggaaattgc ccaacttacc ctcgagcaat
    11581 tccaggcaca aggtcgcccg gaatatgaca ttcgtgtcca ggaattgcaa gtggaactgg
    11641 cacaaatgga gctggatgaa gtgctgacaa gcctgggcat tgacccggac tcagaagcac
    11701 tggatgaact ggatgctcag gtagcacaag ctcgcgcctt tgcacccgca gatggcacaa
    11761 tcatcgcgtc cgtcaacgtg ggacgaaacg tgaatccatc tactccggcc tttgtgctgg
    11821 gcgaccctga cgagcttgaa atcgtggctg aactcgacgc cagtaaaggt gatgaagaag
    11881 ttagggaaat gttcgagggc atgccggtgg tcgtgacgct ggatgcaaag cctgacacag
    11941 aactgaccgg caccattcga cagctcccct ctccgtatgg caccggtgaa gccgatgaac
    12001 gcgtcatcca tatcgtcatg gattcccctc cctccgccag cacttaccag tcaggcgaca
    12061 aagttacagt cacagttctg cttgccagca aacagggcgc gctctggtta ccgcccgagg
    12121 cgattcgcca ggcaggtggt cgaacctttg tcattatcaa cactgaaagt ggacccagcc
    12181 gcgtcgacat cgaaattgga ttgatcactc gcgatatggt ggaaattctc tccgggctca
    12241 cggaaggtca ggttgttctc gggtcatgag cctcttttca ggtttgtctt ccttcgccct
    12301 gcagttctgg gcaaccgcag gcattgcttt taaacgcctg ctcacgcagc gctttctcag
    12361 cctcgcgtca atagccgggc tgatgatcgc atcagggttt attttgagtg tgccgctgta
    12421 tgccgatgcg acctattttc gattgttgcg cgaagaacta ctggccggtc gggaatctga
    12481 attaacacaa aagcccgtag actacgctcc gctttcgttt atttttgaac taaaggcggc
    12541 tggcagaggc agcccgcaat ggaagaatgt tgttgacgta gatacgtatc tctcgagcac
    12601 agccctgcga acgattgatc tgccgatttt gcaggtagtc cgccgttttc ggacggatgg
    12661 ctacaacatg tacgcacccc gtgacccaag taacacgggc acgcaatatc cacttaccac
    12721 cgccaacctc gtcttcatga cgcctttgga agaaaacata attattttga acggcaggac
    12781 tccacagcca tttgtatccc tgcttcgtga aggtgatgcc attgaggcga tggccagtga
    12841 gaacctggca gatgagtttg gcgtacaaac cggtgacatt tacacactgc gcacagacaa
    12901 tggtccgctt ccaatcacga ttgtaggctt atggcgaccc gctgatccat tggcgcctta
    12961 ctgggatgtc aaaagagata cctggctttt cgttcacgaa gaaacctata aaagtatcat
    13021 cagcgatacg gttgatgatg aattgatcag ttcaacctgg agcattgtcg ccgatggctc
    13081 gtcactccac gccaatgatg tggatgggtt gaaagaacga atcctggcga tcgaaagccg
    13141 ggcagctacc ctccttccca agacagcctt gatcgcctcc ccactggatg cgcttgaacg
    13201 ttatcaagca aacgccccaa cgctgactta tcttttgttt gctttcagcg ttcccatact
    13261 gagtctgatc cttgccttca tcgggctggt cgcgggtctc ttcgtcggtc agcaacgcgg
    13321 cgagatggcc atcctccgca gccgcggcgc gagcgcattc caggttgtga gcatgtcgct
    13381 gatgcagggc gtcttactgg ccattgtggc tctggcaggc ggaatcgcat tggcatactg
    13441 gatcacgcat tccatcggca aggcacgcag tttcctcgac tttagcgccg tcggtggact
    13501 acgagttgat ttgaccccgc aagtgcttgg ttatgggttg ctgggcatcg caattattct
    13561 ccttatccag gtgctgctcc ccacgttgag tgccgcagaa aatacgatcg tcacctataa
    13621 acaggaacgt gcccggtcct tccgcaatcc gtggtggcaa aaatattggt tggatattct
    13681 gctactcatc cctgccgggt atggtttatg gcaacttaca caacagagca aagaggcatt
    13741 aggcggatcg gaaacgattc ctgaccctct gcagaatccc cttctcttgc ttgttcctgc
    13801 actggggatt ttttcagtcg ctctctttac gcttcggctt gttccccgct taatggactt
    13861 tgtctcatgg gctcttcaca cctcaaaaag cgtggggtta ttaatggcgt ctcgctatct
    13921 tgcacggact cccgcatttt atagcgcgcc actcgttttg cttgtgttga ctctcggctt
    13981 atcggcattc actgcttctc ttgcccgaac actggatagc cagcttaaca aacaaatgcg
    14041 ttatcaagtg ggtgcggata tcaatattta tgagtttggc acaacgttca atgaagatag
    14101 tccgaatccg gtttacagtt ttgttcccgt cgaagatcat ttgtttatcc ccgaagttcg
    14161 atctgcaaca cgcgttgggc gatacaaatt ttcggcgatt ggccaatctg gctccattca
    14221 aggcgcattt cttggtattg atcgtctcac ctttccttcg gcagcatact ggcaaagaga
    14281 ctttgcatct gaacagcttg gcgttctgat gaatcaactt gcggcagatc ccaatgggat
    14341 tcttgtccca accagcctgc ttgagcaaca aaacctggag ctcggcgata ctctcaaact
    14401 ggcgattacc accggtgttg caggcgagtc cattcctctc gaggcgcata ttgtaggatc
    14461 gtttgacttg ttcccaacct ggtaccctga aactggaaca atgatcgtcg gcaacctgga
    14521 agaaatgttt ttgcgagcag gtgcagaata cccacatgaa gtgtggctgg ggatgaatcg
    14581 cagcgcggac ccggaattga tcgtctatgc tgttcgtgga ttcagtatat tgcttgaccc
    14641 gcaggcagat caaacaaaac ttgtacaaaa cgggttgaac acaattgtcc gggaatggtc
    14701 atcagccgaa ctgaagattc gcgacgaaca acgccgcccc gaacgacaag gattattcgg
    14761 gttactctcc gtcggattta tcgcatcggc attgttgacc gtcctgggct ttttacttta
    14821 tgccttgttc tccttccgcc gccgttttat tgaaatgggc atgctgcgcg ccatcgggct
    14881 ctccattaaa cagatgacat ccctgctcgc cgcagaactg gcgttccttg ttttgcttgg
    14941 catcggtgtc gggacggcgc ttggtgtatt tgcgagcagg ttatttgtcc catttttgca
    15001 gattggagcc agcgctcagg cacagtatcc gccatttcag attgagatcg cctggctttc
    15061 gattgttgag atctatgtat tgtttgttat cttatttatt gtagccctga gcgcgctctc
    15121 agcattgctg gtgcgcatga agatcttcca ggcgatcaaa cttggggaga caacttaatg
    15181 agtgtcgaac ctttgatcgt ttgcgagaac cttgtcaaaa tctaccgact cggctccgcc
    15241 agtcagggca gtgggacttc gattgaggta caggctttac aaggtctgga tatcacagtt
    15301 gcaaacggag agatgatcgg tattgtcg
//
LOCUS       DASTJP010000176         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1290113, whole genome shotgun sequence.
ACCESSION   DASTJP010000176 DASTJP010000000
VERSION     DASTJP010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290113"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..684)
                     /locus_tag="VFQ23_05340"
     CDS             complement(97..684)
                     /locus_tag="VFQ23_05340"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9906037.1"
                     /translation="MEVKPVVLTGQHVRLEPMSEAHVPGLAELGIGQPFWDFMVYGKI
                     NSVDDMQNWVRDVLGRAEKGTDLPFAAIHLESGRVAGATRYLNIMPIDHGLEIGGTWY
                     GPEFQRTVVNTECKYLLFKHAFETLGCIRVQLKTDSRNERSQKAIERIGAVKEGVLRN
                     HMILPDGHYRHSVYYSVLDIEWPEVKNRLEEMMKR"
     gene            complement(675..2264)
                     /locus_tag="VFQ23_05345"
     CDS             complement(675..2264)
                     /locus_tag="VFQ23_05345"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET9906038.1"
                     /translation="MSTTKSLQHIFAELPLPFCMVGIPDIPIRGISIDNRAVKPGYLF
                     VALKGTNVDGHDYIQKAIDNGAAAVVGDREINGLSVPYIQLENPRRALTWIAASFYDW
                     PGRKLTVIGVTGTDGKTTTTNLIYQILLAAKIKAGMISTVNAVIGDEVLDTGFHVTTP
                     DAHDVQRYLAQMVDAGLSHVVLETTSHGWSQYRVDACEFDIGVVTNITHEHMDEHGNY
                     ENYRAAKARLFSSLEKTSEKPQGNPRLGVINRDDSKSFDFLNDFIKVRKLNYGLDDAA
                     DIQAVDVQYSPSGIHFTAKSKDFSVPVSSKLVGAYNVSNCLAALTATVYGLKIKPEIA
                     ARGIASLDGIPGRMERIDLGQNFTAIVDFAHTPNALQVALEAGRTMTNGRVISVFGSA
                     GLRDKEKRRMMAEISAELADLSLLTAEDPRTESLDGILEEMAAGARSRGGREGETFWR
                     VPDRGEAIKFALRLARPGDIVLSCGKGHEQSMCFGAREHLWDDRTAMRAALSEFLGVE
                     GPKMPYLPTQDTHEEEWLKWK"
BASE COUNT          604 a          637 c          497 g          595 t
ORIGIN      
        1 cacaaatagc agaatctcat tgaaatagaa taatcaaaat tacaatagcg gcgcgaaatc
       61 gcgccgcttt tgattcatga ttgatctaaa tcgttcttac ctcttcatca tctcttccaa
      121 acggtttttg acctccggcc actcgatatc caaaacactg taatacacgg aatggcggta
      181 atgcccatcc ggcagaatca tatggttgcg aagaacgcct tccttcaccg cgccaatacg
      241 ctcaatggct ttttgagagc gttcattacg agagtcggtt ttcaactgca cacgaataca
      301 gcccaacgtt tcgaaggcat gcttaaacaa taaatacttg cattcagtat tgaccaccgt
      361 ccgctgaaac tcaggtccat accacgtgcc accgatttcg agcccgtggt caattggcat
      421 gatattcaga taacgagttg caccagccac ccggcccgat tcaagatgaa tggctgcaaa
      481 aggcaggtcg gttcccttct cggcgcgtcc aagcacatct ctcacccaat tctgcatatc
      541 gtccacagaa tttatctttc catacaccat gaaatcccaa aatggctgac cgatgccaag
      601 ttctgctaat ccggggacat gcgcctcgct cattggctca agccgcacgt gctgtccggt
      661 caatacaacg ggttttactt ccatttcaac cactcctctt catgtgtatc ttgcgtaggg
      721 agataaggca tcttcggtcc ttccacgcca agaaattcgc tcaaggcggc acgcatagcg
      781 gtccgatcat cccataggtg ctctctcgca ccaaaacaca tggactgttc atgtcccttt
      841 ccgcaggata aaacgatatc gccgggacgg gccaatctta gtgcaaactt gatcgcctca
      901 cctcggtcgg ggacgcgcca aaatgtttcg ccttcgcgtc cgcctcttga cctcgcgccg
      961 gctgccattt cctccaggat tccatccagt gactcggtcc gcggatcttc agccgtaagc
     1021 aaactcaagt ccgccagctc tgccgatatc tcagccatca tccgacgttt ttctttatct
     1081 cgcagaccgg ctgaaccaaa aacagaaatt actcttccgt tcgtcatggt gcggccggct
     1141 tcgagggcaa cttgtaacgc gttcggcgta tgggcaaagt ctacaatcgc agtgaagttc
     1201 tgacccaggt caattcgttc catacggcca ggaattccat ccaatgacgc aatcccccgc
     1261 gcagcgatct caggcttgat tttcaatcca taaactgttg ccgttagtgc cgcaagacaa
     1321 ttggatacgt tataggctcc aaccaattta cttgaaaccg gaacagaaaa atctttgctt
     1381 ttggccgtaa agtgaatccc agatggacta tactgcacat cgaccgcctg gatatctgca
     1441 gcgtcatcca aaccataatt tagttttctt accttgatga aatcgttgag gaaatcaaag
     1501 gacttcgagt catcccgatt gatcacccca agacgaggat taccctgcgg cttttcagac
     1561 gtcttttcca ggctggaaaa taatcttgcc ttcgccgcgc gataattttc gtagttgcca
     1621 tgttcatcca tatgttcgtg cgtaatattg gtaacaacgc ctatgtcaaa ctcacatgca
     1681 tctactcgat attgggacca gccatgagag gttgtttcca ggacaacgtg tgataaccct
     1741 gcatccacca tttgcgcaag ataacgctga acgtcatgtg catccggcgt cgtaacatgg
     1801 aaccctgtat ccagtacttc atcgccgatc accgcattaa ccgtggaaat cattccggct
     1861 tttatctttg cagctaacag gatttgataa ataagattcg tcgtcgtcgt tttgccatcc
     1921 gtaccagtga cgccgatcac ggtcaacttg cgaccaggcc agtcgtagaa cgaggccgcg
     1981 atccaggtca gggcgcgacg tggattttcc agttggatat atggaacaga gagaccgtta
     2041 atttcacgat cacccaccac agccgcagcc ccattgtcaa tcgccttttg aatgtaatca
     2101 tgaccatcga cattggtgcc ctttagcgcc acaaaaagat agccgggttt cacagcgcgg
     2161 ttgtcaatgg aaatcccgcg gatcggaata tctggaatac ccaccatgca aaatggcaga
     2221 ggcagttcgg caaagatatg ttgtagagat tttgtagtag acatggaatt aaaaaggctt
     2281 tgattatttt accaaagcct tcatacaaga taaaaacctg acaggttttt ttt
//
LOCUS       DASTJP010000177         5433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1131899, whole genome shotgun sequence.
ACCESSION   DASTJP010000177 DASTJP010000000
VERSION     DASTJP010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131899"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..764)
                     /locus_tag="VFQ23_05350"
     CDS             complement(156..764)
                     /locus_tag="VFQ23_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906039.1"
                     /translation="MNRIKLVTTILAILITACAPQATPTINPVDVQNTAVAAAFTLVA
                     ETQAAMPTATSLPPTETATQTPAPTNTSLATVTLATTLTPVAALASTSTSGGDYCQTR
                     ILSWSPKGRPATIHIINTNRAAITVSLFLSETADHFECGYRVYEIGSRSDVFITDMVQ
                     GCYTLWAFNNDQRTPVNAFGSGCINNPDKWTIEITQDRVKGF"
     gene            complement(775..1425)
                     /locus_tag="VFQ23_05355"
     CDS             complement(775..1425)
                     /locus_tag="VFQ23_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906040.1"
                     /translation="MKRNALIIGVVLSLFLSACGGAQATPTVNPVDIQHTAEAAAFTM
                     VAQTQAAIPTATSLPPTEAPTLTPLPTLTPITSASTSTTLDAQNLTPVGLTVTPQTNI
                     PTFTPQAGSGADPCNQPLTGWDGPSANLNIVNETRPRGTIILSLYVVTELGQCGYLYV
                     TGESVTGPIGQYSAGAFVEGPKDFKVFGGFRITQGSWDIVVRNDNIVALGGCYPSC"
     gene            complement(1471..2142)
                     /locus_tag="VFQ23_05360"
     CDS             complement(1471..2142)
                     /locus_tag="VFQ23_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906041.1"
                     /translation="MKKITVALLAMYILSACLPFATNTPAPGPSAPAVDVAGTVQAGA
                     QTSVAQTLTAMPTVTAPSISDTATLPVALPSDTPTIVPETPTLVPNLTTTPVTATAGT
                     LLPALTSTATIAVPPNSATLTALPTLTIRTYGTLPPAVPYASVTLVNKARTEAYISLQ
                     VTTVQGGPTIIEYPVEGTVRIQAPTGFYLYVAWVGGRKLVGEFRLRSTDELTITLFRD
                     RVEIK"
     gene            complement(2202..2546)
                     /gene="trxA"
                     /locus_tag="VFQ23_05365"
     CDS             complement(2202..2546)
                     /gene="trxA"
                     /locus_tag="VFQ23_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9906042.1"
                     /translation="MSSEPIHVTDAAFEKTVLESSIPVIVDFWAPWCGPCKMVAPILD
                     KLAKEYEGKILIAKINTDENPEWMMKYGIQGIPTMLFVSNGKIVHRQVGALPERMLRD
                     TVTQFMDVITQS"
     gene            2665..3579
                     /gene="ftsY"
                     /locus_tag="VFQ23_05370"
     CDS             2665..3579
                     /gene="ftsY"
                     /locus_tag="VFQ23_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET9906043.1"
                     /translation="MIDAFSKWRSGLAKTSKTAFGQLATLLGATEITQETWDDLEALL
                     IQADLGIELTAEIIEELQRVVQTQGLIRSEELSDALRIELRDRLAAPPTLHWTVRPSV
                     IMVVGVNGSGKTTTIAKLAQRFRSQGKTLLFGAADTFRAAAVDQLQVWGDRLKVDVIA
                     GEPESDPGAVAFNTVQSGTARGIDLVFIDTAGRLHTRFNLMEELKKVDRVIGKALPGA
                     PHATWLVLDATTGQNALQQAKAFKDAVRVTGVILSKLDSSARGGMAFAIQRELGLPIL
                     FAGLGEKPEDLMPFDPDAFVEGILGTGT"
     gene            3646..4729
                     /gene="prfB"
                     /locus_tag="VFQ23_05375"
     CDS             join(3646..3711,3713..4729)
                     /gene="prfB"
                     /locus_tag="VFQ23_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114679478.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET9906044.1"
                     /translation="MQDLVNRLKSSQDLTQQLLVRLDFASKELKLSSLEKEIEAPDLW
                     GNPSQAQQLMKQYNTLKNEVESWRSFSQRLRDALELSQLDDESLRADLETEISAIESE
                     LETRSFTAMLSGRYDHDSAILAIHAGAGGTDSQDWAAMLERMYLRWAESRGFQTELLD
                     ITEGEEAGIKSATIAVEGEYAFGYLRSEKGVHRLVRLSPFDAAHRRHTSFALVEVLPQ
                     VAMDEAEIAIDPGDIKMDVYRSSGAGGQNVQKNATAVRITHIPTGIVVTCQNERSQTQ
                     NREFAMRILRARLLELKQAEKEEERAVLRGEYTKAEWGSQIRSYVLHPYQMVKDHRTD
                     YETGNTQAVLDGSLDDFMESFLRSAA"
     gene            4738..4983
                     /locus_tag="VFQ23_05380"
     CDS             4738..4983
                     /locus_tag="VFQ23_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9906045.1"
                     /translation="MSETISKLSEFISGTILKQPNRKIDPSEPLISSGLIDSFSLMDV
                     ALFVEDNFGVRIEDTELNADTFDSLTQLASLIESRKA"
     gene            4980..>5433
                     /locus_tag="VFQ23_05385"
     CDS             4980..>5433
                     /locus_tag="VFQ23_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9906046.1"
                     /translation="MTTFPERLKTLHLQTPDRVAIYLQFAGGEDVPLTYDQLLRGANA
                     YASTLVREGVKPGEVVVLILQHGQDLVYSFWGAILHGAIPSIMPFLTEKLSPERYRAD
                     LSTLVSVTQPAAIITYVEFAGEVRTVLKTDHSDCIVITTEQIETQAELN"
BASE COUNT         1325 a         1274 c         1437 g         1397 t
ORIGIN      
        1 gtaacaagca atgccaaaat aactgcgatg actacggatt tcatttcaac tcctttaagg
       61 gactgtatca aggacgctta acacttgtac attgttcctc aacataaaaa ggcagactct
      121 tcgcctgcct tttgtttgaa ctcactaatt tagaattaaa agcccttgac tctatcttga
      181 gtgatctcaa tcgtccattt atcggggtta ttgatacaac cggatccgaa ggcattcacg
      241 ggagtccgtt ggtcattatt gaaagcccac aaggtataac agccctgtac catatctgtg
      301 atgaaaacat cgctgcggct gccaatttca taaacgcggt atccgcactc aaagtgatct
      361 gctgtttcac ttagaaatag ggatacggta atcgccgcgc ggtttgtatt aatgatatga
      421 atcgtagcgg gtctgccttt gggcgaccag gaaaggatgc gggtctggca ataatcgcca
      481 ccagacgtgc tggtgctggc taaagctgca acgggcgtaa gtgtggtagc cagtgtaacc
      541 gtagcaagtg acgtgtttgt tggggctggc gtttgcgttg ctgtttcagt cggagggaga
      601 gaggttgccg tcggcatggc ggcctgcgtc tcagccacta gcgtgaatgc tgctgcaaca
      661 gcagtatttt gaacatcaac cggatttatc gtcggcgttg cctgcggcgc gcaggcggtg
      721 ataagtatcg ccaggatggt tgtaacgagc tttatgcggt tcatcgagat catcttagca
      781 actaggataa catccgccca gcgcaacaat attatcgttt ctaacaacaa tatcccaact
      841 gccctgggta atgcggaatc caccaaatac tttaaagtct tttggtcctt ccacgaacgc
      901 gccggcgctg tactgcccaa tagggcctgt gacgctttca ccggtcacat agagataacc
      961 gcattgaccc agttcggtca ccacgtataa ggataggatg attgttcctc ggggtctggt
     1021 ctcattgaca atatttagat tggcactggg accatcccag ccggtcagtg gctgattgca
     1081 cggatccgct cccgacccgg cctgaggcgt aaaggtcggg atgttcgtct gaggcgtgac
     1141 cgttaatccg accggcgtta aattctgtgc atcgagcgtt gtcgaggtgc tcgcagaagt
     1201 gatcggagtt aatgtgggca acggcgtgag cgtgggtgct tctgtgggtg gcagtgaagt
     1261 tgcggttggg atggccgcct gggtttgtgc caccatagtg aaagctgcgg cttcagcagt
     1321 atgctgaata tctactggat tgacagttgg agttgcttga gcaccaccac aggcactcag
     1381 gaacaaactt aatacaacgc caatgatcaa cgcgtttcgt ttcataattc ttattcactt
     1441 cctttttgat tttgagattg ctactgaagt ttacttaatt tcaactctat ctctaaacaa
     1501 tgttattgtg agttcatctg tgctgcgcaa cctgaattcc ccgactagct ttctgccgcc
     1561 cacccaggcg acgtaaagat aaaagcccgt cggcgcctga atccgtaccg tcccttctac
     1621 agggtattca atgatcgtcg gaccgccctg aacggtagtg acctgtagtg agatgtaagc
     1681 ctcagttctg gctttattaa ccaaagttac gcttgcatag ggtacagcag gaggcaaggt
     1741 tccgtacgta cgaatcgtca gcgttggtaa agcagtcaaa gttgccgagt tcggcgggac
     1801 agcaattgtg gcggtcgagg tcagtgcggg gagtaaagtt ccggcggttg cagtgaccgg
     1861 agtagttgtc agattgggaa cgagagtcgg agtctcagga acgatcgtag gggtgtcact
     1921 tggcaaggct accgggagag tagctgtgtc tgaaatggaa ggagcagtga ccgtcggcat
     1981 agccgtcagc gtttgcgcga cggaggtttg cgcgccagcc tggaccgtcc cagctacatc
     2041 gacagcgggt gcactggggc cgggtgccgg ggtgttagta gcaaaaggca ggcatgcgct
     2101 caggatatac atggccaaca aggcaacagt aatctttttc atcacgctct ccttttgtct
     2161 ctcaggttcg ctcggttcag attaaaacca ataatgcaga actacgactg ggttatgaca
     2221 tccatgaatt gggtgaccgt atctcgtagc atccgttcag gcaatgcgcc tacctggcga
     2281 tgcacaatct tgccgtttga aacgaacagc atggttggaa ttccctgaat accatatttc
     2341 atcatccatt cggggttttc atcggtattg atctttgcaa tcagaatttt gccttcgtat
     2401 tccttggcca gcttatccag gatcggtgca accatcttac agggaccaca ccatggtgcc
     2461 cagaaatcta caatcacggg aatagatgat tctagaacgg ttttttcgaa agcagcatcg
     2521 gttacatgaa ttggttcact tgacataact tataccttta cttaaatctt ctttgcaact
     2581 gcaaaggatt ggcaattaga atgattttgc cctcaatcga aaaatgagta tatcatagaa
     2641 tgccccatgt tataatcgcc cgtcatgatt gatgcctttt caaaatggag gagcgggctt
     2701 gcgaaaacca gcaaaacagc gtttggacag cttgctacct tattgggtgc taccgaaatc
     2761 acacaggaaa cctgggatga tctcgaagcg ctactcatac aagccgatct gggaatcgag
     2821 ctaactgcag aaattatcga ggagcttcag cgagttgttc agactcaggg cttgattcgc
     2881 tccgaagaac tttccgatgc gcttcgtatt gagctccgcg accgacttgc tgctccgcca
     2941 acgctacatt ggactgttag accttccgtt attatggtcg tgggagtcaa tggttcaggc
     3001 aaaacgacca cgatagcaaa gttagctcag cgattccgct ctcagggaaa aacgctgctc
     3061 ttcggcgctg cggatacctt ccgcgcggcg gcggtggatc agcttcaggt ctggggagac
     3121 cgtctcaagg tggatgtgat tgcgggtgaa ccagaatcgg acccgggagc agtcgcattc
     3181 aataccgtac agtctggtac tgcacgtggt attgacttgg tctttattga tactgcaggg
     3241 cgtttgcata cgcgcttcaa cctgatggaa gaattgaaga aggttgatcg cgtgatcggc
     3301 aaagcgttgc ctggtgctcc tcatgctacg tggctcgttc tggatgccac gaccggacaa
     3361 aatgcactgc agcaggcaaa agcattcaaa gatgcggttc gtgtaacggg tgtgatcctt
     3421 tcgaaactgg attcgtcggc ccgcggtggt atggcatttg caattcagcg tgaactggga
     3481 ttgcctatat tgtttgccgg tctgggtgag aaacccgaag acttaatgcc tttcgacccc
     3541 gacgccttcg ttgaaggaat actggggact ggtacgtaaa ttttacgatg gtggagacga
     3601 aatcaatcgc tgatctcttg tctcgactct ctatctggag gatacatgca agaccttgtt
     3661 aatcgcctta aatcttcgca agacttaacc cagcaactac tggtgcgtct ttgacttcgc
     3721 gtctaaagaa ttaaaacttt cctcacttga aaaagagata gaagcgcctg atctttgggg
     3781 gaatccatcg caggctcagc agttaatgaa acaatacaac acgctaaaaa atgaagtgga
     3841 gtcgtggcgt agtttttcac aacgcttgcg cgatgctttg gagctttctc agcttgatga
     3901 tgaaagcctg cgtgcggatt tggaaacaga aatttctgcg attgaatcgg aactggaaac
     3961 gcgttcgttc accgctatgc tttcaggcag atatgatcat gactcagcca tccttgccat
     4021 tcacgcgggc gccggcggaa ccgattcaca ggactgggcc gcaatgctgg agcgtatgta
     4081 tttgcgctgg gcagaatcgc gcgggtttca aacagaactg ctcgatatta cagaagggga
     4141 ggaagccggt atcaaaagtg caaccattgc ggtcgaaggt gagtatgcct tcgggtacct
     4201 tcgctccgaa aaaggtgtgc accggcttgt gcgcctttcc ccgtttgacg ccgcacatcg
     4261 caggcatacc tcttttgcac tggttgaggt gttgccgcaa gtcgcgatgg acgaagccga
     4321 aatcgcgatt gatcccggtg acatcaaaat ggatgtctat cgttcctccg gtgcaggtgg
     4381 gcaaaacgtc cagaagaatg ccaccgcggt ccgcattacg catattccaa ccggtatcgt
     4441 tgtaacctgt cagaatgagc gttcacagac acaaaaccgt gaatttgcga tgcgtatttt
     4501 gcgggcgcgt cttttagaac tgaaacaggc cgaaaaagag gaagaacggg ccgtgttgcg
     4561 tggcgaatat acaaaagccg agtgggggag tcagatccgt tcgtatgtgt tgcatcctta
     4621 tcaaatggtc aaagatcatc gcaccgacta cgagaccggc aatacgcaag ccgtcttaga
     4681 cggtagtctg gatgatttca tggagtcttt tctacgttcc gcagcttaag gagaagtatg
     4741 tctgagacaa tttcaaaact ttctgagttt atttcgggaa cgattctgaa gcaacccaat
     4801 cgcaagatcg atccatcgga accgctcatt tcaagcgggc tgatcgattc gttcagtttg
     4861 atggacgtgg ctttgtttgt agaagataat ttcggtgttc gcattgaaga tactgaactc
     4921 aatgcggaca ccttcgatag tctgactcaa ctggcgtcgc tgattgaatc ccgtaaggca
     4981 tgacgacttt tccagagcgg ctgaaaacac ttcatttgca aactccagat cgggtggcga
     5041 tctacctgca gttcgcgggt ggtgaggatg tgccgctcac atacgaccag cttcttcgtg
     5101 gagcaaatgc gtatgcttcc actttggttc gtgagggggt gaaaccaggc gaggtagtcg
     5161 tgctgatcct tcagcatgga caggatctcg tctattcgtt ttggggagcg attctacacg
     5221 gcgcaattcc ttctattatg ccgtttctaa ccgagaagtt atccccagag cgctaccggg
     5281 ctgatctatc tacattggtc tcagtgacac agcctgccgc catcattacc tatgtggaat
     5341 tcgcgggtga ggtccgaacg gttttgaaaa cggatcattc agactgcatt gtaattacga
     5401 ctgagcaaat tgaaacacaa gctgaactga att
//
LOCUS       DASTJP010000178         6821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_867477, whole genome shotgun sequence.
ACCESSION   DASTJP010000178 DASTJP010000000
VERSION     DASTJP010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867477"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..546
                     /locus_tag="VFQ23_05390"
     CDS             307..546
                     /locus_tag="VFQ23_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906047.1"
                     /translation="MDMPKPLTTMNLEAARRAERLPGYILALANTELTYQGMLEQDGN
                     CEVCGAHCSRQTTPVFHYQLQEVFFCQTCSQVLID"
     gene            complement(760..1743)
                     /locus_tag="VFQ23_05395"
     CDS             complement(760..1743)
                     /locus_tag="VFQ23_05395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9906048.1"
                     /translation="MEYARLGWTGMKVSRICLGCMGFGDAERWVHKWVLNEENSRPII
                     KKALELGINFFDTANVYSIGASEEILGRALKDFAKRDEVVIATKVHGKMREGPNGGGL
                     SRKAILSEIDHSLKRLGTDYVDLYIIHRWDYETPIEETMEALNDVVRAGKARYIGASA
                     MFAWQFQKALHVAEKHGWTRFVSMQDHLNLLYREEEREMLPLCRAEKIGVTPYSPLAS
                     GRLTRDWSSESTLRSETDQIAKMKYDTTTETDRQVVERVAELAEKHGVPRTHIALAWL
                     LQKAPVTAPIIGATKISHLEEAVGALSVKLTPEEVTYLEEPYSPHSIVGHQ"
     gene            complement(1768..2730)
                     /locus_tag="VFQ23_05400"
     CDS             complement(1768..2730)
                     /locus_tag="VFQ23_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9906049.1"
                     /translation="MNSNLRGAISLLLAMLIFSLQDIAVKWIGGDYPVLEIVVIRSLI
                     ALPCTFPFLWYEGIRGIPVTKQPALEYIRGFFLFLSFTTYMMGLVALPLADIAAIRNS
                     GPLMITLLSVLWLGERVGPRRWVALIVGFIGVLLIIQPGSGTFNLGSIFVLLATLFYT
                     LSVMITRKLQSTDSSATMAYYSSLVYLAVGFILAPLAILFGEPPNAHPSIAFLFRAWT
                     LPTLLDLILMAGLGLVWAGGMYFVARAYSLAQASVVAPFEYAPLLINIVWGVLLWHEV
                     PTWMTLAGAFLTLSSGLYILYRERRITSEIDQVSDEPYPVSTKA"
     gene            complement(2783..4246)
                     /locus_tag="VFQ23_05405"
     CDS             complement(2783..4246)
                     /locus_tag="VFQ23_05405"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9906050.1"
                     /translation="MANNNVFKRTNPEAEGISSIAVLDFIRAVEQHNHPLDAVQGFML
                     LRHGNVAAEGWWSPYGPEHPHTCYSLSKSFTSTAIGLAVAEGRLTVDDPVLKYFPDDA
                     PANPGENLKAMCVRHLLSMNTGHGEDTTLRIFRGEDDNWARAFLALPVEYQPGTWFVY
                     NTAATYMLSAIITQLTGGTLLDYLRPRLLDPLGIENPFWETDPRGINLGGTGLHIKTE
                     EIARFGQLYLQRGMWQGKRIVPEGWIVEATQVTSDNSNTQTNPDWAVGYGYQFWRCRH
                     NAYRGDGAFGQFCIVMPEQEAVLTMIGGVQEMQAVLDKVWEHLLPAMQPEALPENSQA
                     YNELCDKLDRLPLPLANGQAASPGMEQWSGKTYTLETNHLMLQSAAIEFGDTGSTLTL
                     RNDRGEHLISIGQSAWLQGTSDWRGRGKELLATSGAWTAEDTYEVRICCNEDAYCPIL
                     RFNFNGSELRLEVSPNATWDWEPAGITRIIGRVTVEA"
     gene            complement(4308..5159)
                     /locus_tag="VFQ23_05410"
     CDS             complement(4308..5159)
                     /locus_tag="VFQ23_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9906051.1"
                     /translation="MQTVKLNNGLEIPILGFGVFQITDQAECERSVIDAIEVGYTHID
                     TAASYMNEEAVGRGIKQSGVEREKLFITTKLWIQSNGYEGTLRAFERSLKRLQLDYLD
                     LYLIHQPFGDVYGEWRAMEELYQQGKVRAIGVSNFQPDRIIDLMIHNKITPAVNQIEV
                     NPFHQRIDTQAFLQDNNVKVQAWAPFAEGKNHIFQNETLLSIAAKYNKSVAQVILRWV
                     TQRGIIALAKSTRKERMVENFNVLDFELSADDVAAIGTLDTNTTSFFDHRNPEMVKWL
                     GTRKLDV"
     gene            complement(5196..5579)
                     /locus_tag="VFQ23_05415"
     CDS             complement(5196..5579)
                     /locus_tag="VFQ23_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9906052.1"
                     /translation="MMENVNQEQEVIHLSKEKWDWMAARKVETLDALFHEKSVFVHMG
                     GSWGKEREMEIIRSGGIHYKQAEIHEVSVNVIGTTAVLLNRITLLAVVGGNEVTNPFM
                     VTEVYVQQEGSWKLASLSFTKLLTP"
     gene            complement(5580..5966)
                     /locus_tag="VFQ23_05420"
     CDS             complement(5580..5966)
                     /locus_tag="VFQ23_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9906053.1"
                     /translation="MKIAEVSEQYAISPDTLRYYERVGLIPPVNRNDSGIRDYNELDL
                     RRVDFIKCMRRAGLPIDVLIEYVALVQKGDKTIEARKDILIEQRDLLMARMEEMQKTL
                     DILNHKVEVYENAVLKKEKQMIPIKE"
     gene            6114..>6821
                     /locus_tag="VFQ23_05425"
     CDS             6114..>6821
                     /locus_tag="VFQ23_05425"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9906054.1"
                     /translation="MKTGDLITTAIESMVEAGALAGAATLIWRDGTIAQVATVGWRDV
                     EARLPIERDTLFRIASMTKPITSVAALMLFDDGRFALDDSITRWAPEFSQMRVLRSPD
                     ADLEQTYPAERQITFRDLLTHRSGLTYGAFHTGPISKAYEEALGGEIDSPVAPDDWIR
                     NLASLPLIDQPGSGFHYGRSTDLLGLLIARIEDAPLGDVLKRRIFDPLGMKDTGFSVP
                     KEKKDRRAGMYGFDEAGR"
BASE COUNT         1590 a         1828 c         1684 g         1719 t
ORIGIN      
        1 tcctggtagc ctcgactgga ctggttctga attgttgttg catcattatt ccactgacat
       61 actaaccgag gcagaacaag ggagcgggta taaaaaccga cggagcaata tcgcagcctg
      121 catgcagttg gatctgtgcg accgtgttgc gcagtgatga agtttgtttt cgcaatgaga
      181 ttttttaata gccttccctc aggagcacgt tcccgagggt tgtgctttat agggtatatc
      241 ccccatagtt cagtttgatt agatgaaata aaattcgcag aattttctgg aaggagtcga
      301 aacacaatgg acatgcctaa acctttgacc actatgaatc ttgaagctgc ccggcgcgcg
      361 gagagactgc ctggttatat ccttgccttg gctaataccg aactgaccta ccagggcatg
      421 ctcgagcaag acggtaattg cgaagtgtgc ggcgcgcact gctcccgaca aacaacgccg
      481 gtatttcact accaattaca agaagtgttc ttttgtcaga cgtgcagcca ggtactgatt
      541 gactagggcc gagtgagcgt ggtttgcaaa ttcacattgt aggctagctg caaggcgcag
      601 catgccttga tctttgtata ctattgtttg gtaataatta ttactgcaca tcccccagca
      661 gaccaaagcg ggcgttgggg gatgtgcctt taaaaccacc tcaacacaat ggttgagttg
      721 aggatagatc tcaggtcggg atatcacaaa tacactgtct cactgatgac ccacaatgga
      781 atgcggcgaa tagggttctt ccaggtatgt gacttcttcg ggagtcagtt tgacggacag
      841 agccccaacg gcttcttcca gatgcgagat tttcgttgct ccgataatgg gcgccgtcac
      901 tggcgctttt tgcaacagcc aggccagtgc gatatgcgtg cgcggaacgc catgcttctc
      961 cgcaagttct gcgactcgtt ccaccacttg tcggtcggtt tcggtcgttg tatcgtattt
     1021 cattttggcg atttgatcgg tttcggaacg aagtgtgctc tctgatgacc agtcacgcgt
     1081 caatcttcca gaggcgagag ggctatatgg ggttacgccg atcttctccg cgcggcagag
     1141 tggcagcatt tcgcgttctt cttcacggta gaggaggttc agatgatcct gcatcgaaac
     1201 gaagcgagtc cagccatgct tctcagcgac atgcagcgcc ttctgaaact gccaggcgaa
     1261 cattgcggaa gcaccaatgt atctggcctt gcccgcccgt accacatcgt tcagcgcttc
     1321 cattgtctcc tcgatcggag tctcataatc ccaccgatgg atgatgtaga gatcgacata
     1381 atccgtcccg aggcgtttca ggctgtgatc gatctcgctc aagatagcct tgcgcgacaa
     1441 gccaccgccg ttgggtcctt cgcgcatttt gccatgcacc ttggtggcga tgacgacttc
     1501 atctcgcttt gcgaaatcct ttaaggcgcg acctaggatc tcttcactcg ctccaatgga
     1561 atagacgttg gcggtatcga agaaattgat ccccagttca agggcctttt tgatgatggg
     1621 acgactattt tcctcgttca atacccattt gtgaacccag cgctcggcat ctccgaaccc
     1681 catgcagcca agacagatcc gtgagacttt catccctgtc caacctagtc gagcgtattc
     1741 catgttctct cctatctgat cttcttatca agccttggtg ctgaccgggt atggttcatc
     1801 actgacctgg tctatttcag atgtaatcct tcgttctcga tacaaaatgt acagtccgct
     1861 ggatagcgtc agaaacgcac ccgccaaggt catccatgtg gggacttcat gccagagcag
     1921 aaccccccac acaatattaa tgagcaaggg tgcatattca aatggtgcaa caacggaggc
     1981 ctgagccagg ctgtaggcgc gcgcgacaaa atacatccca cctgcccaga ctaaccccaa
     2041 gccagccatg aggatcagat ccagcagcgt tggcaaggtc caggcgcgga acagaaaggc
     2101 aatgctcgga tgggcattag gtggttctcc gaatagaatc gccagcggcg ccaaaataaa
     2161 cccgacagca aggtaaacca acgaactgta ataagccatg gtcgcgctgc tatcggttga
     2221 ctgtagtttg cgggtgatca tgacacttag cgtgtagaaa agcgtagcca gcaatacaaa
     2281 aatcgaaccc agattgaagg ttcccgaccc tggctgaata ataagcagga caccgataaa
     2341 accaacgatc aatgcgaccc aacggcgtgg gcccaccctc tcccccagcc acaggacgga
     2401 taagagggtg atcatgagtg gtcccgaatt gcggatggcg gcgatatccg ccagcggcag
     2461 cgcgactaaa cccatcatgt acgtggtgaa agacagaaac agaaaaaaac cacggatgta
     2521 ttcgagcgcg ggttgcttcg tgactggtat tccccgtatc ccttcatacc acaaaaaggg
     2581 aaaggtacac ggcagggcaa tcaggctgcg aatcacgaca atctccaaaa ccggataatc
     2641 gccgccaatc cacttgaccg cgatatcctg cagggaaaag atcagcattg ccaacagaag
     2701 ggagatagcg ccgcgaagat tggaattcat gttgctcttt ttagtcgctg aacttgttga
     2761 ggcaagtgag gaaaattttc actcacgctt ccacagtcac gcggccgata atccttgtga
     2821 tgccggcagg ttcccaatcc caggttgcat tgggacttac ttcaagtcgc agttcgctgc
     2881 cgttgaaatt gaaacgcagg attggacaat acgcgtcctc gttgcagcag atgcgtacct
     2941 catatgtgtc ttctgctgtc caggctccgg aggtggcaag gagttccttg ccgcgtccgc
     3001 gccaatcgct ggtcccttgc agccaggcgg actgaccaat cgatatgagg tgttcgccgc
     3061 gatcattgcg taatgtcaat gtgctgcctg tatcaccaaa ctcgatcgca gcactctgca
     3121 gcatgagatg attggtttct agcgtatatg ttttccccga ccattgctcc atgcccggcg
     3181 aggctgcttg tccgtttgcc agaggcagtg ggagacgatc cagcttatca cacagttcat
     3241 tgtaggcttg tgaattttca ggaagagctt cgggctgcat cgcaggcagt aaatgctccc
     3301 acaccttgtc gagcactgcc tgcatctcct gcacgccgcc aatcatggtc agcaccgcct
     3361 cctgctctgg cataacaatg cagaattgac cgaaagcgcc gtcaccgcga taggcgttgt
     3421 gtcgacagcg ccagaactga tagccatagc ccactgccca gtctggattg gtctgcgtat
     3481 tgctgttgtc ggaagtcacc tgtgttgctt caacgatcca tccctccggc acaatgcgct
     3541 tcccctgcca catgcccctc tgcaaatata actgaccgaa gcgagcaatc tcctccgtct
     3601 taatatgcag gccggtaccc ccgaggttaa tgccgcgcgg gtcagtctcc caaaacggat
     3661 tctcaatgcc cagaggatcc agaagacgcg ggcgcagata gtcgagtagg gttcctcccg
     3721 tcagctgcgt aatgattgct gaaagcatgt aggtggccgc ggtgttatac acgaaccatg
     3781 tgccaggctg atactcgacg ggtaacgcca ggaaggcccg cgcccagttg tcgtcctcgc
     3841 cacggaatat gcgcaaggtt gtatcctccc cgtgaccggt gttcatggat agcaggtgcc
     3901 gcacgcacat cgctttcagg ttctcaccgg ggtttgcagg tgcatcgtcc ggaaaatact
     3961 tcagcacagg gtcatccact gtgagccgcc cttcagccac tgccagtccg atggcagtag
     4021 aagtgaagct cttgctcagc gagtagcatg tatgcgggtg ttccggtcca tagggactcc
     4081 accagccttc ggcggccaca ttcccgtggc gcagaagcat gaaaccttgc actgcatcca
     4141 agggatggtt gtgctgctca acggcacgga tgaaatccag aaccgctatg gaggagatgc
     4201 cttctgcttc cggattggtg cgcttaaaca cgttgttatt ggccattatg ccgcctactc
     4261 cttttctcac gttttagaac tccatgataa ccgtgattgc ctgtctatca gacatctaat
     4321 tttctagtac ccagccactt caccatttca ggattgcgat ggtcaaagaa actagtggta
     4381 tttgtgtcta acgttcctat cgccgccaca tcgtccgcgc ttaattcaaa atcgagcacg
     4441 ttgaaatttt ctaccattcg ttctttacga gttgattttg caagagcgat aatccctctt
     4501 tgtgtcaccc accgcagaat aacctgggca acgcttttgt tgtatttcgc ggcgatcgaa
     4561 agtagagttt cattttggaa gatatgattc tttccctctg caaatggagc ccaggcctgc
     4621 accttaacat tgttatcttg tagaaacgct tgtgtatcaa ttcgctggtg gaatggattt
     4681 acttcaatct gattgaccgc gggcgttatt ttgttatgga tcatcaaatc tatgattcga
     4741 tccggttgaa aattcgaaac cccgatcgct ctgactttgc cttgttggta tagttcttcc
     4801 atcgcacgcc attccccata cacatcgccg aagggttgat ggatcaggta cagatcgagg
     4861 taatccagtt gcaatcgctt cagtgacctt tcaaaagccc ggagggtgcc ctcatagcca
     4921 ttgctttgaa tccaaagttt tgtcgtgatg aacaattttt ctctttcaac gccgctttgt
     4981 ttgattcccc tgcctaccgc ttcttcattc atgtaagaag cggcggtatc aatatgggta
     5041 tatccaactt caatggcatc gatcacgctt ctttcacatt cggcttgatc tgtgatctga
     5101 aagacaccaa aaccaagaat ggggatttca aggccattgt tcaattttac agtttgcatg
     5161 attttctctt tatgtttccg tggttgatgg tgtcgttatg gggtaagcaa tttggtaaac
     5221 gaaagcgacg ccaacttcca gctaccctct tgttgtacat agacttctgt caccatgaaa
     5281 ggattggtga cctcgtttcc gcccaccacg gcgagcagcg taattcggtt taaaagcaca
     5341 gcagttgtac caatgacatt caccgacact tcatggatct ccgcttgctt gtaatggatg
     5401 cctccgcttc tgatgatctc catttcacgc tctttgcccc atgatccgcc catgtggacg
     5461 aagacagatt tctcatggaa gagtgcgtct aaggtttcta ccttgcgcgc tgccatccag
     5521 tcccacttct ctttagagag gtggattact tcctgttcct ggtttacatt ttccatcatt
     5581 cattccttta tcggaatcat ttgtttctct tttttcagca cggcattttc atacacttca
     5641 accttatggt tcaagatgtc cagcgttttt tgcatttctt ccattctggc catcagcaaa
     5701 tctcgttgct ctatgaggat atcttttctc gcttcaatgg tcttatcccc cttctgcacc
     5761 agcgccacgt attcaatcaa cacgtcaata ggaagcccgg ccctccgcat acatttgatg
     5821 aaatccactc gacggaggtc gagctcgtta taatcgcgaa tgccgctatc attccggttg
     5881 accggtggga taagacctac acgctcatag tagcgaagcg tatctggaga aattgcatac
     5941 tgttcactga cttcagctat tttcataatt cacctcgagt gtgagtctaa cacctggagt
     6001 cgactccaag tcaagggcct ggttctcacc gcaaggttgt aatcaccgaa gtggtatcat
     6061 cgccccatga cttccgctaa taaaactata ctttactccc cagagaaacc aaagtgaaaa
     6121 caggagatct catcacgacc gccatcgaat cgatggtcga ggcaggcgcg ctggcgggtg
     6181 cagcgaccct gatctggcgg gacggtacga tcgcccaagt tgcgaccgta ggctggcggg
     6241 atgtcgaagc caggttgcca atcgagcgcg ataccctgtt tcgtatcgcc tccatgacga
     6301 aaccgatcac ctctgtggcc gccttgatgc tgttcgacga tggacgcttc gcgctggatg
     6361 attcgatcac gcgctgggcg ccggagtttt cacagatgcg ggtcctgcgt tctccggacg
     6421 cggacctcga gcaaacgtac ccggcagaac gacagatcac gttccgcgat ctgttgaccc
     6481 acagatcggg cctgacctac ggtgcatttc acacaggacc catctcgaag gcgtatgaag
     6541 aagcattggg aggcgagatt gacagccctg tcgcacccga tgactggatc cgaaatctgg
     6601 cttccttacc cttaattgac caacctggca gcggttttca ctacggccgc tccaccgacc
     6661 tgctcggcct gcttatcgcg cgcatcgaag atgccccgct gggcgatgta ctcaaacggc
     6721 ggatttttga cccgctcgga atgaaagata ctggcttcag tgtaccgaag gagaaaaaag
     6781 accgacgagc gggaatgtac ggttttgacg aggcggggcg c
//
LOCUS       DASTJP010000179         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1290051, whole genome shotgun sequence.
ACCESSION   DASTJP010000179 DASTJP010000000
VERSION     DASTJP010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290051"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1438
                     /gene="hutI"
                     /locus_tag="VFQ23_05430"
     CDS             164..1438
                     /gene="hutI"
                     /locus_tag="VFQ23_05430"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET9906055.1"
                     /translation="MLIHSSSQLLTLAGGSQRGKKLGTLGIIENGAVLIRDEKIIAVG
                     TTDELKAAYPDEPTLDAGGCVLMPGFVDPHTHIIWAGDRANEFELKMAGTPYLDILAA
                     GGGILSTVKQTRTASIETLIAQTRPRLLRMFAHGTTTAEAKTGYGLQTATELRLLKAL
                     LALDDESPLDLSITFLGAHAIAPEYKDNPQGYTDLVCDTMLSMLKEWWETHAPRLPLP
                     FVDVFCETKAFDLEQSRQILTKARSLGFPLKIHADEFDNLGGASLAVELGAASADHLV
                     RTSDADIAALGKSDTVAVSLPCTPFGLAEKDYTPAHKLIEADAILALATDCNPGTSWN
                     ESMQFVIALACRTMKLTPAQAIAAATINSAHAIRRSDLIGSIEVGKQADLLILSVPDY
                     RHLGYRYGTNLVRQVIKRGKVYSVDVGYYRTA"
     gene            1526..1903
                     /locus_tag="VFQ23_05435"
     CDS             1526..1903
                     /locus_tag="VFQ23_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9906056.1"
                     /translation="MVDCAIKVHRILGPGLLESAYQGCHAYELRKRGRDVKTEVKLPI
                     IYEKQRIDVGYRIDTWVDDLIVIENKVVESLRPIHQAQILTYLKLSGCKIGFLLNWNV
                     RLMKNGIRRVVLGLDGQTPYPRK"
     gene            2005..3696
                     /locus_tag="VFQ23_05440"
     CDS             2005..3696
                     /locus_tag="VFQ23_05440"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HET9906057.1"
                     /translation="MTSVNFSPGKTLLNNQWLAFATVLILNAVIALLIAVLLSRRLAT
                     PGRNAMICMLIGLAVWAFCYAMITLSTSLEAKRFWLKLENIGILTVPVFWFFFTLQYT
                     RLDKWLNKYTAAVFLIIPVVSWIILFSGSWFHFYYASVSQMSEGSGPLLIERGPWYLV
                     ALIHSYLLNLLGMGLLIWQVIEYRNIYREQMAILVSAILIPWLTNVFYQVASGFNSSS
                     STTLIDLTPISFTVTAFLISAGVFGLRLFDLMPIARHVVIEYIPEMVVVVDAHDRVLD
                     ANKTAQQWLGKTLDEIVGKDPMEVFSKWPELLNRFLASHETHEEIQIHGDPPLTLDLD
                     VSALYNRFNVLEGRVIVAHDATEHKWLENDLKYANEALIRQLEEIQKLRDELEQQAIR
                     DPLTNVYNRRYMVEFLENEIARAEREKLPVSIVMMDLDNFKQFNDNYGHKCGDMILKA
                     FANFLLEHTRRADVVCRYGGEEFVILMPNTSHQIGYERAEMWRQDFSETPVEYEGIKF
                     SITFSAGVATFPQHGHTGDLILQAADRVLYRSKDGGKNKVTAYLSRKEIKDAKDF"
     gene            3708..>3922
                     /locus_tag="VFQ23_05445"
     CDS             3708..>3922
                     /locus_tag="VFQ23_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET9906058.1"
                     /translation="MQIDIIGVPIDLGADRRGVDMGPSAIRYANLRHRLTELGHTVED
                     KGNIDVPIQETCEVTDPKLKYIDCIIP"
BASE COUNT          984 a          974 c          942 g         1022 t
ORIGIN      
        1 cgggattcct cctggcttcg atgtttttgc gattatagtt ggatacggac tgacaggatg
       61 atagccgtaa caaaagcagt atagcactac ttctccattt gcatctctca atgtgtggtt
      121 aaattacctc acgaatctcc agtctctaat ctccaattac cccatgctca tccactcctc
      181 ctcccaactt ctcaccctcg ccggcggatc tcaacgcggc aaaaaactcg gcacacttgg
      241 cattatcgaa aatggtgcgg ttctcatccg cgatgaaaaa atcatcgcag tcggcacaac
      301 ggacgaactc aaagccgctt atccggatga gccaactctc gatgcaggcg gatgtgtctt
      361 gatgcctggc tttgtcgatc cgcatacgca tatcatttgg gcaggcgatc gcgccaatga
      421 gttcgagttg aaaatggctg gcacaccgta tcttgatatc ctcgccgctg gtggtggaat
      481 actctcgaca gtcaaacaga ctcgtacagc gtcgatcgaa acacttatcg cacagactcg
      541 cccacgtctc ctgcggatgt tcgcgcatgg cacaacaact gctgaagcca agaccggcta
      601 tggactccaa accgcgaccg agcttcgcct actcaaagcc ttgcttgcac tcgatgacga
      661 atccccgctg gacctctcta ttactttcct cggcgcgcac gccattgcac ccgaatataa
      721 agataacccc cagggctata cggatctcgt ctgtgacacg atgctgtcta tgctcaagga
      781 atggtgggag acacacgcgc cgcgcttacc tctgccgttt gtagatgtct tctgcgagac
      841 aaaagctttt gacttagaac aatcgcgtca aatcctgacc aaagctaggt ctttgggatt
      901 cccgctcaag atccatgcgg atgagtttga caacctcggt ggcgcatcgc tggcggtgga
      961 actcggagcg gcgtctgccg atcacctcgt cagaacctct gatgcagaca tcgccgcgtt
     1021 aggaaagtca gatacggtgg cggtctcttt gccgtgcacg cctttcgggc tcgccgaaaa
     1081 agattacaca cccgcgcata aattaatcga agcggatgca atccttgcct tagccacaga
     1141 ttgcaatccc ggtacctcat ggaatgagtc catgcagttt gtgatcgcgc tggcctgtcg
     1201 aacgatgaaa ttgactcccg cgcaagccat cgccgccgcg acgatcaatt ctgcgcacgc
     1261 cattcgcagg tccgatttga tcggttccat tgaagtcggc aaacaagccg acctgctgat
     1321 cctgtctgta cccgattacc gtcatctcgg ctatcgctat ggaacgaacc tggtcagaca
     1381 ggtgatcaaa cgggggaaag tctattcggt agatgtgggg tattatcgaa cagcctaatt
     1441 ctttattcac cgcaaagagg cgaaggacgc taaggttctt aaggaggaag atgcgctgaa
     1501 ggatatcaag gcgattgcta cagacattgt tgattgtgca attaaagttc atagaatcct
     1561 tggacccgga ttgctggaat ctgcttatca aggatgtcat gcatacgaac tgcgcaaacg
     1621 tggcagagat gtgaaaaccg aagtcaaatt gcctattatt tacgaaaaac agagaattga
     1681 tgtaggctat cgaatcgata cctgggtaga cgacttgatt gttattgaga ataaggtagt
     1741 cgagtctctt cgcccaatcc atcaagctca aatactaacg tacttgaaac ttagtggctg
     1801 taaaattgga tttctgctca attggaatgt gagacttatg aaaaatggaa ttcgacgcgt
     1861 ggttcttggt ctggacgggc agactcccta tcccagaaag taaattaacc tcctgctttt
     1921 ccttcgcctt aataaataat ccttcgcgct cttcgttcct tcgcggttta aattgtttac
     1981 aacagagtaa aattcgtctg acgggtgact tccgtcaatt tttcgccagg taagactctt
     2041 ttgaacaatc aatggctggc cttcgcaacg gttcttattc tgaacgcagt gattgcgctg
     2101 cttatcgcgg ttttattgag tcgaaggctt gccacccccg gccgaaatgc catgatctgc
     2161 atgttgatcg gtttggcagt ttgggcattt tgttatgcca tgatcaccct ttcaacatcg
     2221 ctcgaagcca aacgtttttg gctcaaattg gaaaacatcg gcattctcac cgtgccggtg
     2281 ttctggttct tttttaccct gcaatataca agactcgata aatggttgaa taaatacaca
     2341 gctgccgtgt tcttgatcat cccagttgta tcgtggatca tcttattttc ggggagttgg
     2401 tttcattttt actatgcctc tgtctctcag atgtcagaag gcagtgggcc tttgctgatc
     2461 gagcgcggtc cctggtatct ggtagcgctg atccattcct atttgctgaa cttgctggga
     2521 atggggttgc tcatctggca ggttattgaa tatcgaaata tatacaggga gcaaatggcc
     2581 atcctggtca gtgccatttt gattccctgg ctcacgaacg ttttttatca ggttgcctct
     2641 ggattcaatt cctcctcatc gactacattg attgacctga cacctatttc attcacggtg
     2701 acagcctttc tgatcagtgc aggtgtgttt ggattgcgcc tgtttgatct gatgcctatt
     2761 gcgcgccatg tggtgatcga atacattccc gagatggtag tggtcgtgga tgcacatgac
     2821 cgtgtcctcg acgctaataa gactgcccag caatggctgg gaaaaacact ggatgaaatc
     2881 gtgggcaagg acccgatgga ggtattcagt aaatggcccg aactgctcaa tcgctttctc
     2941 gcctcccatg aaactcacga ggagattcaa attcacggtg acccgccgct caccctcgat
     3001 ttagatgtct ctgccctcta taatcgcttc aatgttttgg aaggtcgtgt catcgttgcg
     3061 catgatgcga cagagcacaa atggctcgag aacgatttga aatatgcgaa tgaagcactc
     3121 attcggcaac tggaggagat ccaaaagctg cgcgatgaat tggaacagca ggcgattcgg
     3181 gatccactga caaacgttta caaccgccgc tatatggtcg aatttctcga aaatgaaatc
     3241 gcgcgagcgg agcgtgaaaa actgccggtc tccattgtga tgatggatct ggataatttc
     3301 aaacagttca acgacaacta cggccataaa tgtggagata tgatcctcaa agccttcgca
     3361 aactttctgc tagagcacac ccggcgcgcg gacgttgttt gtcgctatgg cggcgaggag
     3421 tttgtgatcc taatgccaaa tacctcgcat cagatcggat atgaacgcgc cgaaatgtgg
     3481 cgacaggatt tttcagaaac tcctgttgag tatgaaggaa taaaattttc aatcaccttt
     3541 tcggctggag tggcaacctt tccccagcac ggtcatacgg gcgatttgat cctccaggct
     3601 gccgatcgag ttttataccg ttccaaagat ggcggtaaga acaaggtaac agcgtacctc
     3661 agtcgtaaag agattaaaga cgcaaaggat ttctaaggag ataattcatg caaatagaca
     3721 tcatcggtgt gcccattgat ctcggtgcag accgccgcgg tgtagatatg ggtcccagtg
     3781 caatacggta tgccaatctt cgtcatcggc ttaccgaact aggacatacc gtagaagata
     3841 agggcaatat cgacgtgccg attcaggaaa cgtgtgaggt caccgaccca aaactcaaat
     3901 atattgattg catcatcccg at
//
LOCUS       DASTJP010000180         5038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_465276, whole genome shotgun sequence.
ACCESSION   DASTJP010000180 DASTJP010000000
VERSION     DASTJP010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465276"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFQ23_05450"
     CDS             <1..593
                     /locus_tag="VFQ23_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906059.1"
                     /translation="TFLDASWFYYPFSNYRANRIADHLSKFVNTNEVVLDCGCGSMLV
                     SEILQQRFGVKAFGADVIRLNHNNHHFCLCSGERLAFPNAYFDAVFLIFTLHHISNPV
                     QALEEGLRVAKKRLIVLEDVYQNQQEFQLLKMLDWYGNIMTSKEMPFPYNFKTEDEWK
                     NIFQSLGTKLIAVENIRPNPWRPSRHRLFVLEKNHI"
     gene            665..1471
                     /locus_tag="VFQ23_05455"
     CDS             665..1471
                     /locus_tag="VFQ23_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906060.1"
                     /translation="MKKHKGLFSFFIFITVIAITSLSCGVSDISGMFATETSTPTQTF
                     TPSPTFTPSPTPTSTQTPSPTPLPTGVEVLDQPDGSTKFVDYDNKFEIALPADWFILP
                     LSAEDIGEIMSQAATENPDLAQAVEVFKQLDPDVIRVMAINTDSNYVYRDFSTNISVT
                     ALKDDLLSSMPVAFVTGVLQSQVEKGGATLIPVEDFAITNSNGVEIGLLEFEQQAPTA
                     SGATITVQSRVLIFQSGGSLINIQLAVPEQFAAELFPVMDEIAESIKVLE"
     gene            1610..3262
                     /gene="hutU"
                     /locus_tag="VFQ23_05460"
     CDS             1610..3262
                     /gene="hutU"
                     /locus_tag="VFQ23_05460"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET9906061.1"
                     /translation="MPGPRTVRAPRGVDLTCKNWLSEAAFRMIQNNLDPEVAEKPDDL
                     VVYGGRGKAARDWESFDAILESLKNLEEDETLLVQSGKPVVVFKSHKDAPKVLIANSN
                     LVPHWATWEHFDELAKKGLIMYGQMTAGSWIYIGTQGILQGTYETFGALAKLKGWGTL
                     KGKFVLTAGLGGMGGAQPLAITMNEGVGLIVEVDPERAERRRAIGYVDTVVDTLEEAM
                     TLVEEAKDGQIPKSIGLIGNAADIYSELVARGVTPDVVTDQTSAHEALFYIPSGLSVT
                     EADELRKSDPDTYKKMAMESMSKHVRAMLDFQRAGAEVFDYGNNLRQQAYNNGTSDAF
                     EFPGFVPAYIRPLFCEGKGPFRWVALSGEEQDIYTTDEAIMELFPEDDHLKRWLKMAR
                     EKVPFQGLPARICWLGYGERVRAGLKFNELVASGKVKAPIVIGRDHLDSGSVASPNRE
                     TESMKDGSDAISDWAILNALINAVGGATWVSFHHGGGVGMGYSQHAGQVIVADGTLEA
                     ARRLERVLTTDPGMGVVRHADAGYEEAIEAAKRHGIKMPMLK"
     gene            3515..3847
                     /locus_tag="VFQ23_05465"
     CDS             3515..3847
                     /locus_tag="VFQ23_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9906062.1"
                     /translation="MEDNELVQNMLLELRRGILSLAVLSQLSTEQYGYSLLKALSDQG
                     MEIDQSTLYPLLRRLESQGLLQSDWRIVDEARPRRYYVISAQGKAVLTKLKGEWSTMV
                     ETMKQMLS"
     gene            3862..4869
                     /locus_tag="VFQ23_05470"
     CDS             3862..4869
                     /locus_tag="VFQ23_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906063.1"
                     /translation="MKLIDRYVAQVGKNLPAKTREDIEKELKSTLEDMLEDRAQKLDR
                     PADEAMELELLREYGAPDKVADTYYSMQYLIGPRMYPTFIMVLKIVFTVLSALAVVGL
                     VVALGKVGFAGREFAQTLMQTVGGYIGAAITAFGNVVLVFVLLERFLPDSELKELKIA
                     EEWDPTTLLKEQNPDIVKRGELIAEIIFTFAAVVILNFYPQIIGISFPVDGEWFTIPM
                     FSDAFFRFLPWINLTLLAEIILDIYLLRQNIWSRSTRLVKVIVEASNAAITIAILNTP
                     GILGFTAESFAGSPINAETAKTLTTVFTTMFPIVLSIVLIIQAFELAKAIYQLVKSRP
                     S"
BASE COUNT         1301 a         1196 c         1297 g         1244 t
ORIGIN      
        1 acaccttttt ggacgctagt tggttttatt atcccttttc aaactatagg gcaaaccgta
       61 ttgctgatca tttgtctaaa tttgttaaca ccaatgaagt tgttctggat tgtgggtgcg
      121 gctcaatgct ggtttcagag atcttacagc agcgatttgg ggtgaaggca ttcggcgcgg
      181 atgtgatccg tttgaatcat aacaaccatc atttctgttt atgttccggg gaacgcctgg
      241 cgttcccaaa tgcatacttc gatgcggtct ttctgatctt cacactgcat catatttcga
      301 atccggtcca ggccctggag gaaggtctca gggtcgcgaa gaaaaggtta attgttcttg
      361 aagatgtcta ccagaaccaa caggagttcc aacttctaaa aatgctggat tggtatggca
      421 acatcatgac ctcaaaggaa atgccattcc cttacaactt caagacggaa gacgaatgga
      481 agaacatttt ccaaagcctg ggcacaaaac tgatcgctgt ggaaaatatt cggccaaacc
      541 catggagacc ctctcggcat cggctgtttg tgttggaaaa aaatcatatt tgaattcaat
      601 cgatccttcg cggctataat ggctgggagg gatccttgat caaagaagcc caaaggaggg
      661 ccacatgaag aaacacaaag gtttgttcag cttctttatt ttcataacgg tcattgccat
      721 taccagccta tcttgtggag tttcagatat ctcgggtatg tttgctacag aaacgtccac
      781 cccgactcaa acattcacac ccagccccac atttacccca agccccacac cgacatcgac
      841 acaaacaccc tctccgaccc cactgccgac aggtgtggaa gttctcgatc aacccgatgg
      901 ctccacaaaa ttcgtcgatt acgataacaa attcgaaatc gctctccctg cagactggtt
      961 tatccttccg ctcagcgcgg aggatatcgg cgaaatcatg agccaggcag caacagagaa
     1021 tccagatctc gcgcaggctg tagaggtgtt caagcagctc gatccggatg tgatccgggt
     1081 gatggccatc aacacagact cgaactatgt ttacagggac ttttccacca atatcagcgt
     1141 aacagccctg aaagacgatc tattgtcttc gatgccagtt gcatttgtta ctggagtgtt
     1201 gcagtctcag gtggagaaag gcggcgccac tttgattccg gttgaagact ttgccatcac
     1261 gaactcgaac ggcgtagaaa ttggcttgtt ggaattcgag cagcaagctc ccactgcctc
     1321 cggggcgacg atcactgtcc aatccagggt gcttatcttt cagtcaggcg gttcattgat
     1381 caacatccag ttggccgtgc cagaacaatt tgctgcagag ttattccctg taatggatga
     1441 aattgctgag tcgatcaaag tactcgaata gatcgaagga aattcaacag aatccctccc
     1501 ggcgtataat cgggagggat gttattttaa ccctgtcatt tccgagacca tcgaaggaat
     1561 atcttatgac tcttcgttgg gctcaaagtg acccagagga gagtgcatca tgcccggacc
     1621 tagaaccgtt cgcgcaccga ggggcgtgga cctgacctgt aaaaactggc ttagtgaagc
     1681 agcatttcga atgatacaga ataatctcga tcccgaagta gcggagaagc cagatgatct
     1741 cgtcgtctat ggtgggcgcg ggaaggcggc gcgcgattgg gaatcatttg atgcaattct
     1801 tgaatcactt aaaaatcttg aagaggatga aacgcttctg gtgcaatccg gcaagcctgt
     1861 agttgtattc aaatcacaca aagatgcacc caaggtgtta atagcaaatt cgaatttggt
     1921 tccccattgg gcaacctggg aacattttga tgagctcgcc aaaaaggggc tcatcatgta
     1981 cggtcagatg acagctggct cctggatcta tatcggtacg cagggcattt tgcagggcac
     2041 ctacgaaacc tttggcgccc ttgccaaact caaaggctgg ggaacgttga aaggaaaatt
     2101 tgtactgacg gcaggtctcg gcgggatggg tggcgcgcaa ccgttggcaa ttaccatgaa
     2161 tgaaggcgtt gggctgatcg tcgaagtgga ccctgaacgt gcagaacgcc gccgcgcgat
     2221 tgggtatgta gataccgtgg ttgatactct ggaagaagcg atgaccctcg tggaagaagc
     2281 caaggacggg caaattccaa aatcgatcgg cttgatcggc aatgcagcag atatctatag
     2341 cgaactggtt gcgcgaggtg tcacgcccga tgtggtgaca gatcaaacgt ccgcgcatga
     2401 agcgttgttc tatattccat ctggtttatc tgtgaccgaa gcagatgagt tacgaaaatc
     2461 tgatccagat acatacaaaa agatggcaat ggaatcgatg tcaaaacacg tgcgtgccat
     2521 gttggatttt cagcgcgcgg gagccgaggt ttttgattat ggcaataacc tgcgccaaca
     2581 ggcctacaac aatggaacga gcgatgcatt tgaatttccg ggctttgtgc ccgcctacat
     2641 ccgcccgctg ttttgcgaag gaaaaggtcc atttcggtgg gttgcgctct ctggcgagga
     2701 gcaggatatt tacacaaccg atgaagccat catggaatta tttcctgagg acgatcatct
     2761 taaacgctgg ttgaagatgg cgcgtgagaa agttccattt cagggattgc ccgcgcgcat
     2821 ctgctggctg gggtatggtg aacgcgtcag ggctgggctc aaattcaatg aactggttgc
     2881 aagtggcaaa gtcaaagcgc cgattgtgat tggacgcgat catttggatt caggttcagt
     2941 cgcttcgccg aatcgtgaga ccgaatccat gaaagatggc tctgatgcaa tctctgattg
     3001 ggcgatcctc aacgctctga ttaacgcggt tggcggcgcc acctgggtct cattccatca
     3061 tggcggaggg gtgggcatgg gatacagcca gcacgcgggg caggtcattg tggcagacgg
     3121 gaccctcgaa gcggctcgga ggttggaacg agtcctgaca acggaccctg gtatgggcgt
     3181 agttagacat gccgatgccg ggtatgaaga agcgatcgaa gccgcgaaac gtcatggaat
     3241 caaaatgcca atgttgaaat aatctttgtt ctggatgcag ttgtactgca tccagatgcg
     3301 cagcacgctg cgcgccactg ttgtttgaac gacgaagttc aaaagacatc tcgaaaattg
     3361 attttcggga tgtttttgta tgcgtgttca ggatgcaatt gcactgcatg ttgttcgcag
     3421 taaaactgct cacaaacaag tgcatacatc accttatact ttcacccttg acatactgta
     3481 tgatatacag tataatacga catataggtg acatatggaa gataacgaac tcgtacaaaa
     3541 catgcttttg gaactgcgac gtggcatcct gtctcttgcg gtgctgagtc agttgagcac
     3601 agaacaatat ggttattccc tgctcaaggc tctctccgac cagggaatgg agatcgatca
     3661 aagcacactc tatcccctgc tccgcaggct cgagtcgcag ggattgctgc aatccgattg
     3721 gcgcattgtg gatgaagccc gtccgcgccg gtattacgtc atcagtgcac aaggcaaggc
     3781 tgtgctgacc aaactcaaag gtgaatggtc cacaatggtt gagaccatga agcaaatgtt
     3841 gtcgtaaaga aggagaatga aatgaaactg attgaccgtt atgttgccca ggtgggcaaa
     3901 aatcttccgg caaaaacccg ggaggatatc gagaaggaat taaagtccac actcgaggat
     3961 atgctggagg accgtgccca gaaattggac cgtccggcag atgaagccat ggaactcgag
     4021 ttactccggg aatatggtgc ccctgataaa gtggcagata cgtattattc aatgcaatat
     4081 ctgatcgggc cgcggatgta tcccaccttc atcatggttc tgaagatcgt atttaccgtg
     4141 ctaagtgcac tggcagtggt gggactggta gtggcactcg gcaaggtcgg tttcgccgga
     4201 cgtgaatttg cgcaaacact gatgcaaact gttggcggct atattggcgc tgccatcaca
     4261 gcctttggca atgtcgtgct cgtctttgtc ctgctggagc gctttctgcc ggactctgag
     4321 ctaaaggaac ttaagatcgc cgaagaatgg gaccccacca cactgctcaa ggaacagaac
     4381 ccggatattg taaaacgcgg tgagttgatc gccgaaatca tctttacatt cgcggcggtg
     4441 gttatactca atttttatcc acagatcatt ggtatcagtt tcccagtgga tggagaatgg
     4501 ttcaccatcc ccatgttctc ggatgctttc ttccgcttcc tgccatggat caacctgacc
     4561 ctgctggcgg agatcattct ggacatttac ctcctccgtc agaacatttg gtcccgtagt
     4621 acaaggctgg tgaaggtaat tgtggaagcc agcaatgctg caatcacaat tgcgatcctc
     4681 aatacgccag gcatactcgg tttcacagcg gaatcgttcg caggttcgcc gatcaatgcc
     4741 gagaccgcca aaaccttaac taccgtcttc actacgatgt tcccaatcgt actcagcatc
     4801 gtgttgatca ttcaggcatt tgaactcgcc aaagcgatct accagctggt gaaaagcaga
     4861 ccttcttaaa tcacaaagtg ctaagtagcc aggaaaaacc gggcggactt ttgtccgccc
     4921 gatttttttt atttgaccca ggttgccacc tcgatcctga taaatgtttg tcggaatggt
     4981 ttccctttct ccctctggga gatggcaggg tatgcactgc gaccaaagca gtatgtct
//
LOCUS       DASTJP010000181         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_503776, whole genome shotgun sequence.
ACCESSION   DASTJP010000181 DASTJP010000000
VERSION     DASTJP010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503776"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..899)
                     /gene="nrfD"
                     /locus_tag="VFQ23_05475"
     CDS             complement(273..899)
                     /gene="nrfD"
                     /locus_tag="VFQ23_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9906064.1"
                     /translation="MAKGHFVQEPETQIKVCHHSLDRVACFLRNNPPGRACCWHSGLL
                     ERSSIPNLTDLVPWGLWITIDLSSIALAAGAFSLCAAVHLFGLKRYEPIARTATFIGL
                     IGYTLAMLALVLDIGRPDRFWKSLVYWNTHSLLWEVTIAIMLYTTVLVFETMPIFARF
                     EWLRKRFPKIVAKMERIYHYAPILAIIGLGLSSMHQSSLGAAYGIIKG"
     gene            1043..1549
                     /locus_tag="VFQ23_05480"
     CDS             1043..1549
                     /locus_tag="VFQ23_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906065.1"
                     /translation="MMKRIIPPVIIALGIVLSFSAFSLLLKSPSRSPATVDLPAQLAG
                     FSITDSQQGMDAIALITDLHGQEFPVDFGAMGVYGDRDIIVWIAGAASESVAVEMTNA
                     MQRKIAEGNSPFTPIDEIDSRNRKVYALEGMGQRHYYFQSQNLVIWLAANPAFADKAL
                     QQILEVYS"
     gene            1546..1788
                     /locus_tag="VFQ23_05485"
     CDS             1546..1788
                     /locus_tag="VFQ23_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906066.1"
                     /translation="MKKSHIWMMILCCLIPVAGLVAVYFFQIPLSKVVFFGMMLLCPI
                     SHLLMMKFMGHDHSATEQHTQHQHHTSAVTAPTQDK"
     gene            1792..>2229
                     /locus_tag="VFQ23_05490"
     CDS             1792..>2229
                     /locus_tag="VFQ23_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9906067.1"
                     /translation="MAEQLTPENEAFSNPVARSKRTGLIVTFAVLLALLGLLAWGLKK
                     VQAGPVSSGMAPDFNLTSFDGRTLKLSELRGQVVIINFWASWCPPCREEAAYLEQTWR
                     KYEDKGVVFIGVDWVDTEKEALAYMDEFDLTYFNGPDIGTRIAQ"
BASE COUNT          583 a          535 c          572 g          539 t
ORIGIN      
        1 gtgagtggcc ccttggttat caatcagaga ccttcgctcc gacccgggtc aaacgtatag
       61 gacatggaca gagcatccca ggcgcggaag taaaagtatc caatcagaag ccatcccacg
      121 aaacgggaaa cccgttcgat cagtgcatca tttacttttg ctttgggggt tagacgagag
      181 gcaagcattg agacaaagag agtcagttaa atgtctccga caatcgctgc cagcatgaac
      241 agcacagaca tttccggctt gaaccagaat ggtcaaccct tgataatgcc atatgcggcc
      301 ccaagcgagg attgatgcat gcttgaaagg ccgagcccga tgattgccag gatcggcgcg
      361 taatggtaga tacgctccat cttagcgaca atcttcggga agcgtttccg cagccactcg
      421 aagcgtgcaa agatgggcat cgtttcaaag accagtacgg tcgtatacag catgatcgct
      481 atcgtgactt cccatagcag agaatgcgtg ttccaataga cgagagattt ccagaagcga
      541 tcggggcgcc caatgtccaa gacgagggcc aacatcgcca gggtatatcc aatcaagccg
      601 ataaacgttg ccgtccgcgc aattggctca tatcgtttca agccgaacaa atgcactgct
      661 gcgcacaagc tgaatgcgcc agctgccaag gcaatggatg atagatcaat ggtgatccac
      721 aatccccagg gcacgagatc ggtgaggttg gggatactag acctttccag aagaccagaa
      781 tgccagcagc aagcccgccc gggaggatta ttcctaagaa agcaagccac gcgatccagg
      841 gagtgatgac aaactttaat ttgggtttct ggctcttgaa caaagtgtcc ttttgccatt
      901 ccggagctct atacccagtc taggggggaa caaagccaga gtaaatatgt ttagttcccc
      961 attttgctat aagcagattt gcctatcctg ccgatacgcc ttttagcgct tgtgaaatca
     1021 ggtacgcaat aagataatca cgatgatgaa gcgcatcatt ccacctgtaa tcatcgcgct
     1081 tggaatcgta ttgagtttta gcgcgttcag cctcttatta aagagtccct cgagatcacc
     1141 cgctacagtc gacttacctg cgcaactcgc cgggttcagt attacggact cgcagcaagg
     1201 catggatgct atcgctctca taacagactt gcacgggcag gaattcccgg tggactttgg
     1261 tgcaatgggg gtttatggag accgcgacat tatcgtgtgg atagctggcg cagcatcaga
     1321 atccgttgct gtggaaatga cgaacgcgat gcagcgaaag attgctgaag gtaattctcc
     1381 attcacacct attgatgaaa ttgactctcg taatcgcaag gtctacgctc tagaggggat
     1441 gggacaaaga cattattact tccaatcaca aaatctagtt atatggctgg ctgctaatcc
     1501 agcttttgcc gataaggcac ttcaacaaat tctggaggtc tactcatgaa aaagtcacat
     1561 atctggatga tgatcttgtg ctgcttgatc ccggttgcgg gtttggtggc cgtctatttc
     1621 tttcaaatcc ccttgagcaa ggtcgtgttt ttcggcatga tgttgttgtg cccgatttcg
     1681 catttgctga tgatgaagtt catgggacat gatcattccg caactgaaca gcacacgcag
     1741 catcaacatc acacaagcgc tgtgactgca cccacacaag acaagtaacc catggctgaa
     1801 cagctcactc cggagaatga ggcattttcg aatcccgtgg cgcgaagcaa aagaacaggg
     1861 cttatcgtta cttttgcagt tctgctagct ctgctgggac ttctggcgtg ggggttgaaa
     1921 aaggtgcaag ctggtcccgt ttcaagtgga atggcaccag actttaacct tacttctttt
     1981 gacggccgta ccctgaaact cagcgaactg cgcggacagg tggtcattat taatttttgg
     2041 gcttcgtggt gtccgccctg tcgtgaagag gccgcctatc tggagcagac atggcgaaag
     2101 tatgaggaca aaggcgtggt cttcattggc gtcgattggg tggatactga aaaagaagcg
     2161 ctcgcttata tggatgaatt tgatttaacg tacttcaacg gcccggatat aggcactcgt
     2221 attgctcag
//
LOCUS       DASTJP010000182         9598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1184091, whole genome shotgun sequence.
ACCESSION   DASTJP010000182 DASTJP010000000
VERSION     DASTJP010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184091"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..2134
                     /locus_tag="VFQ23_05495"
     CDS             185..2134
                     /locus_tag="VFQ23_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9906068.1"
                     /translation="MLTSENEQDSSFIHREAEREPRRGLRHYLIGRPLPTADAEHETI
                     GKSVGLAVFASDALSSTAYATQEMLVILAAAGAMAFGYVFPISIVIVGLLAIVVVSYV
                     QIIHAYPEGGGAYIVAYDNLGRFPALVAAAALLTDYILTVSVSVSSGVAQIVSAYPQL
                     FQYRVFIAVVCVVFITIINLRGVKESGAAFAIPSYFFIVIMFITVGTGLLRILFGSLG
                     TVVDPPPVEHFGETVSGITAFLLLHAFSSGTTALTGVEAISNGTTAFKEPRSRNASIT
                     LVVMAVILGFLFIGISFLTREVHAVPSENETVISQLARTVFGGEGLPYLLVILGTTVI
                     LILAANTAFAGFPRLSAMLANDGFMPRQLTYRGSRLVYSYGIVALAAVACILIVIFQA
                     SVTRLIPLYAIGVFLSFTLAQSGMTRRWWRAGQLDPNDTSAKHAHNLRYEKGWRFKML
                     SNGFGAFCTAIVMCVFAITKFKDGAWIVLILTPILIAIFLWIQRHYVGVAHSLSLEKY
                     GEPPPYNIRHRIIVPISKVHQGTLAALRYARTLSDDITAVHISLEPEDTEKIRRKWET
                     WGRGTRLVIVDSPYRLFIEPLLDYIDEIIATRQANETITIVVPHFVPAHKFYKFLHMQ
                     TAEILRQELLETPGVIITEVPYQTH"
     gene            2208..2870
                     /locus_tag="VFQ23_05500"
     CDS             2208..2870
                     /locus_tag="VFQ23_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET9906069.1"
                     /translation="MNFIVLGCGRVGAELCYRLHKSGHQVVVVDNNRRAFNRLHPEFR
                     GRTLEGEGLAESVLERAGIREADGLAAVTNSDTLNAVAAHIARAFYDVPNVVARNYDP
                     NLRSIIEAFGLQTVSSTYWGAQRVEELLMNPMQKVVYSPGNGEVEIYEVRIPEEWNNR
                     TLGELLNQLQGCYAVALSSAGRAFLPEPNTTLHEGDLLNVSSTFEGIGALTARLSKKA
                     EA"
     gene            2872..3573
                     /locus_tag="VFQ23_05505"
     CDS             2872..3573
                     /locus_tag="VFQ23_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET9906070.1"
                     /translation="MFVLIAGGGRTGARLANLLINQNYKVRLVENRRELLGRLHQELP
                     TEVIYEGNPIEPSVLEAAGIREVHAVAAVTAEDATNLALCFLGKTMFDVPRTIARVNN
                     PNNAWLFNEKFQVDVALNAADVLAHLIEEEMSLGDMITLFKIRRGRYSVVEEKVPEGA
                     KAIGVQLKDLNLAEHCVIAAIIRDGIMTLPRGDSTLQENDEIVAVASAEGAQKLSELL
                     ADPIYPLREGKKKDS"
     gene            3804..3974
                     /gene="rpmH"
                     /locus_tag="VFQ23_05510"
     CDS             3804..3974
                     /gene="rpmH"
                     /locus_tag="VFQ23_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET9906071.1"
                     /translation="MTKRTYQPKIRRRVRVHGFRKRMATADGRAVLKRRRLRGRHKLT
                     TTANKHVKKVRW"
     gene            4010..4381
                     /gene="rnpA"
                     /locus_tag="VFQ23_05515"
     CDS             4010..4381
                     /gene="rnpA"
                     /locus_tag="VFQ23_05515"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET9906072.1"
                     /translation="MQRKFRLTRSEDFKRVRRSGKSYAHPLVVLIVQTHDKRSNPVGQ
                     TRIKVGVAAGRTVGTAVYRNRAKRLLREAMRALIPDIAPGLDLILIARPGLVSATLED
                     TQRALSNLLQRAQILIPRHES"
     gene            4371..4661
                     /gene="yidD"
                     /locus_tag="VFQ23_05520"
     CDS             4371..4661
                     /gene="yidD"
                     /locus_tag="VFQ23_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET9906073.1"
                     /translation="MNPEFHLHDYSNEPRLRDMPRSIVNWPRILVLSLIRLYQMTLSR
                     GMPANTCRFYPSCSHYGYQAVYKYGALKGSLMAGWRVLRCNPFNPGGYDPVP"
     gene            4678..5847
                     /locus_tag="VFQ23_05525"
     CDS             4678..5847
                     /locus_tag="VFQ23_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9906074.1"
                     /translation="MRKKLFFVSIMLFATVILSGCVGAVNWPGLGADEQTAYLANGQF
                     VYAINASDGSERWHYPLQADSKLMFYSTPVVTDEGLVIIGSSGINHSLIAVNPQKYPD
                     TLPAPVGGVQRFFCGIGLASCPEPVKVEEWIFTGSRDHWVAGPLALDGKLFAPNADGS
                     LYVLDLSDGSLLEKITLDGEPNQPNRLWSQPSTDGERVFVTSLDHSVYAIDIETHDIL
                     WHEDLGGAIPGGVVLASDGMLYAGSLSKQLERFDPATGAHEPVLDTNGWIWGTPVSDG
                     DNLYFSDVDGYFYSYNTVDQKLNWEPVKPDSAITASPLPQSGGILLAAESGNVYSVDP
                     TGKVSLWYDEPSEGKAYTTPVSSDGHVLIAYLESDYLLLALNEQGRQAWTFPSQQ"
     gene            5857..6738
                     /locus_tag="VFQ23_05530"
     CDS             5857..6738
                     /locus_tag="VFQ23_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HET9906075.1"
                     /translation="MKNTTNKTQTEVQRPELPQGLSRESLKTVAVIAILVLAFLRWND
                     VIAILINVMIFIYSLVGQNFGIALILFTILIRLITWPLNAQQMKGAAAMQALQNDKEW
                     QGIQKKYAKDREKLAQEQMRLYRERGINPFGSCLPTLVQFPILFALIPAITFAIGSTP
                     LSILNLSRSVYPFQDVAALVPLNSKFLWMDLGLPERTLLFGLSIPVLALVVALTTYVQ
                     TKLTMPPSANPNDQAAMTGKMMSIYMPIMLYFFSVNYAAGLSVYFVISNILSIIQYAM
                     MGKVNWRNLLPGGQKSN"
     gene            6748..7542
                     /gene="jag"
                     /locus_tag="VFQ23_05535"
     CDS             6748..7542
                     /gene="jag"
                     /locus_tag="VFQ23_05535"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HET9906076.1"
                     /translation="MNEKTTLEIIAPTVEEAIAQGLAELGLTADAVSVEVLDAGNKGL
                     FGFGKHQVRVRLTVNPPGGMQPFEAESVPASVPEVEYSNQRPQAAPSKPVPAAVTKES
                     ESPLEHDPLLDHTEAVISKMLHLLKLEAQVSAHYGAAERDGRRNINVDIRGNDLGVLI
                     GRRSETLAAFQYVASLIVGKDTQQFIQLTVDVEGFRERREKQLIQMAKRMADQVVKSG
                     RRQTLEPMPSGERRIIHIALRDHPAVKTESTGDDPYRKVTIIPKDS"
     gene            7605..8456
                     /locus_tag="VFQ23_05540"
     CDS             7605..8456
                     /locus_tag="VFQ23_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9906077.1"
                     /translation="MSNPVSLQPVDYLLLGHVAHDLTPTGPRLGGTVAYSALTARAMG
                     MRVGIVTAAGPETSLEPLNELSVISLPSTQSTTFENIYTSSGRIQYLRAQAKRLDFSA
                     VPEVWRNSPIIHLGPIANEIESSLPEEFAPGLLGITPQGWMRTWDSGCRVSPIEWANS
                     ESALAQAGAVIISREDVGGNDELIEQYAQQTRVLAVTEGPAGAVLYWHGDRRRFRAPD
                     VNEVDATGAGDIFAAAFFVRLLKTRDPWESTRFATLVASRSVTRVGLDGIPTPEEIEK
                     CLMEILD"
     gene            8467..8904
                     /locus_tag="VFQ23_05545"
     CDS             8467..8904
                     /locus_tag="VFQ23_05545"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HET9906078.1"
                     /translation="MSNQSPSLFRIISTDYSSYLSILFPVVFGFFTIYFFNTENSSSQ
                     LFLLITTAVTAIGIPTLFQRYRTISSVFANGTLTKGVITHIGFLRGRGRVEYSYTAQG
                     EKHTSSNAINRNGRTRNLRIGQEVKVVVDPNDPRRAFIQDIYL"
     gene            8921..>9598
                     /locus_tag="VFQ23_05550"
     CDS             8921..>9598
                     /locus_tag="VFQ23_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9906079.1"
                     /translation="MTRIYTLVNQKGGVGKTTTAINLGAYLAQSNQRVLIVDLDPQAN
                     ATACLGVDKRAVQSGTYEALLNGDLPASSILINERLNLALLPSSPSLAGAEVELVDET
                     GREFRLKNALTSLNGKYDYILIDCPPSLGLLTVNGLVAARDGVLVPVQCEYLALEGLG
                     QITQTIERVQAALFPGLRVRGVILTMFDPRTRLSADVVKEVNNHFPGQVFKSIVPRSI
                     RLAEAPSY"
BASE COUNT         2316 a         2340 c         2442 g         2500 t
ORIGIN      
        1 aaataatgtg tataaagccc ttcacatgca gacggcggaa ctccttcgcc gagagttgct
       61 ttcgacgccc ggcgtgatta tcactgaagt cccttatcaa attccatagc gacaaacgca
      121 ttatgatgat ttgtaatata atcatttcca gtaactacta gggtataggg tcgttgtatt
      181 attaatgtta acttctgaaa acgagcaaga ttcgtcattc attcaccgtg aggccgagcg
      241 agaaccaaga cgcggcttgc gccactatct cattggtcgt ccactcccaa ccgcagatgc
      301 ggaacatgaa acaattggca aatcggttgg cctggcagtt tttgcttctg atgccttatc
      361 ttccactgca tatgctacac aggagatgct tgttatcctc gccgcagccg gggccatggc
      421 gttcggatat gttttcccaa tttcgattgt gatcgtcggc ttattggcca ttgtggtggt
      481 atcgtatgtt cagattatcc atgcatatcc agagggaggc ggggcgtaca ttgttgcata
      541 tgataacctt gggcgttttc cggctctggt tgctgctgct gccttactga ctgactatat
      601 tctcacggta tctgtatcag tttcttcagg tgtggcgcag atcgtatcgg cataccctca
      661 actgtttcag tatcgggttt ttattgcagt cgtatgtgtc gtttttatta caatcatcaa
      721 tctgcgcggg gtgaaggaat ctggcgcggc gttcgccatt ccgagttatt ttttcattgt
      781 gattatgttt attaccgttg gaaccggctt gctgcgtatc ctctttggct ccctgggaac
      841 cgttgtagac ccgccacccg tggagcattt cggtgagaca gtttcaggaa taacagcttt
      901 tcttcttcta cacgcttttt cgagtggtac aacagccttg accggtgtgg aagccatctc
      961 aaatggtacg acagcattca aggaacctcg cagtcgcaac gcgagtatca ccctagtcgt
     1021 gatggccgtc attctaggat ttttgtttat cgggatctca ttcctgacac gagaggtcca
     1081 tgctgttcca tcggagaatg aaaccgttat ctcgcaactt gcgcgcacgg tcttcggtgg
     1141 cgaggggttg ccctatttac ttgtaattct tggaacaacg gttattctga tccttgcagc
     1201 caatactgca ttcgcggggt tcccgcgctt gagcgcgatg ctggcaaatg atggcttcat
     1261 gccaaggcaa ttgacctatc gcggcagtcg gttggtgtat tcctacggga ttgtggcact
     1321 tgcggcggtg gcttgcatcc tgattgtcat ttttcaggca agcgttacgc gcctgattcc
     1381 tctatatgca attggagtgt ttttgtcatt cactcttgcg cagtcgggca tgacgcgtcg
     1441 ctggtggagg gccgggcaac tcgacccgaa cgacacatcc gccaaacatg cacataacct
     1501 acgctatgaa aagggctggc gcttcaagat gcttagcaat ggttttggcg cgttttgcac
     1561 ggctatcgtg atgtgtgtgt ttgcgattac caaattcaaa gatggcgcct ggattgttct
     1621 gatccttacg ccgattttga tagccatctt cctttggatt caacgacatt atgtaggcgt
     1681 agcccatagt ctttccctgg aaaaatatgg ggaaccccct ccttacaata ttcgtcaccg
     1741 aatcatagtt cccattagca aagtccatca gggaacgtta gcagcgctcc gttatgcgcg
     1801 caccttgtct gatgatataa cagcagtcca tatctccctg gaaccggaag atacagaaaa
     1861 gatccgcagg aaatgggaaa cgtgggggcg cgggacccgc cttgtgattg tggattcccc
     1921 gtatcggctc ttcatcgaac cgttactgga ctacatcgac gagattattg cgactagaca
     1981 ggcaaacgaa acgatcacga ttgttgtgcc gcactttgtc cctgctcata agttttataa
     2041 attcctgcac atgcaaacgg cggaaatact gcgtcaggaa ttgcttgaaa ctccgggcgt
     2101 gatcattacc gaagttcctt atcaaactca ttaattggta gctgatacga ttttttaagt
     2161 atggtctttc cctcgcagga gtaaaaggat atgaaggaga aggtattatg aattttattg
     2221 ttcttggctg tggccgcgtg ggtgcggaac tttgctaccg ccttcacaag agcggtcatc
     2281 aggtggttgt tgtcgacaat aaccgtcggg cgttcaatcg cctgcaccct gaattccgcg
     2341 ggcgtactct tgaaggggaa gggctggctg aaagcgtgct cgaacgcgcc ggaattcgtg
     2401 aggcagatgg tttggcagcc gttacaaact cagatacgtt aaatgccgtt gcagcacata
     2461 ttgcgcgtgc cttttatgat gtacccaatg tggtggctcg aaattatgat cctaatctgc
     2521 gctccattat tgaagcgttt ggtttgcaga ccgtcagttc cacgtactgg ggcgcgcaac
     2581 gggtagagga actcctcatg aacccgatgc agaaagtagt ctattctccg ggcaacggag
     2641 aagttgaaat ttacgaggtg cgcattcctg aagaatggaa caaccgcacg ctgggcgaat
     2701 tgctaaacca acttcaggga tgctatgccg tcgctttatc gagcgcaggc cgcgcgtttt
     2761 tgcctgaacc gaatacaacg ctgcatgaag gcgatttgct gaacgtcagt tccacatttg
     2821 aaggaatcgg agcgctgacc gctcgtcttt ccaagaaggc ggaggcataa tatgtttgta
     2881 ttgatcgcag gtggcgggcg aacgggggca cgtctggcca acttactcat caaccaaaat
     2941 tacaaggtcc gcctggtcga aaatcgacgt gaattgctgg ggcgcttaca tcaggaactt
     3001 ccgaccgaag tgatctatga gggcaacccc attgagccca gtgtgctgga agcagcagga
     3061 attcgagaag tgcatgcggt tgctgcagta actgccgaag acgccaccaa tctggctctc
     3121 tgttttctcg gaaaaacaat gtttgatgtg ccgcgcacaa tcgcccgcgt gaacaatcct
     3181 aacaatgcct ggctgttcaa cgaaaaattc caggtggatg tggcgcttaa tgcggctgat
     3241 gtgttggctc atttgatcga ggaagaaatg tcccttggcg acatgatcac tttatttaaa
     3301 atccgccgcg gaaggtattc agttgtcgag gaaaaggtcc ctgagggagc caaggcgatt
     3361 ggcgtccagc ttaaggacct gaacctggca gaacattgtg tcattgccgc gatcattcgg
     3421 gatggaatca tgacccttcc acgtggagac agcaccctgc aggaaaacga cgaaatcgtc
     3481 gcggtagcta gcgctgaagg tgcacagaag ctttctgagt tactggcgga tcccatctat
     3541 ccactgcgag aggggaaaaa gaaggattca taacgttcca caaaaccaaa ttgaccgaat
     3601 cgaaaccctt acggtataat attgtgccag tctgtacaag caggctgtca ttaactgtaa
     3661 atatctgacc tgaaattttc cttactacgc atcaaatttc aagttcaggg attaatacag
     3721 aagaagcatt cttaccgcta gtctcacgat gcacttaaag ttgagagcac tatgcggcac
     3781 tttgtgaagc gaggaacgag gtaatgacaa aacgtacgta tcaaccgaaa attcgccgcc
     3841 gcgtgcgcgt gcatggattt cgcaagcgca tggccacggc tgatggacgc gctgtactga
     3901 agcgccgtcg tctgcgcggg cgtcacaaat taacgacgac cgcaaataag catgtcaaga
     3961 aagtccgctg gtaagcggaa actgaattcg agcgtgttcg agagtccggg tgcagagaaa
     4021 gtttcgtctg acccgatcag aagacttcaa gcgggtgcgg cgttccggta aatcctatgc
     4081 ccacccgctt gttgtgttga ttgtccagac tcatgataag cgaagcaatc ctgtaggcca
     4141 aacgcgcata aaggttggcg tcgcggcagg gcggactgta ggaacagcgg tttatcggaa
     4201 ccgagccaaa cgtctactgc gcgaagcgat gcgggcgctt atcccagata tcgctccagg
     4261 tttggatctg atcctgatcg cccgccccgg acttgtatct gccactttgg aagatactca
     4321 acgcgctctc agcaatcttc tgcaacgcgc gcaaatttta attcccaggc atgaatcctg
     4381 aatttcacct gcatgattat tcgaatgagc cccgtttacg cgatatgccg cgttccattg
     4441 tcaattggcc gcgcatcctc gtgctttcgt tgattcggct ttatcagatg accctctcgc
     4501 gcgggatgcc tgcgaacacc tgtcgctttt atccatcctg ttcacattat ggctaccagg
     4561 cagtctataa gtatggcgcg ctcaagggat cgttgatggc gggctggcgc gtgctgcgct
     4621 gcaatccctt caaccctggt ggttacgatc cggttccata ggagcaatat tttttcgatg
     4681 agaaagaaac tgttttttgt aagcattatg ctgttcgcca ctgtcatcct aagtggatgt
     4741 gttggcgcgg taaattggcc cggtctgggg gccgatgaac agaccgccta cctcgcgaat
     4801 ggacagtttg tttatgcaat caacgcgagc gacgggtctg aacgctggca ttatcctctg
     4861 caagctgatt ccaaactgat gttttactca acacctgttg ttaccgatga aggattggtc
     4921 atcatcggca gttctggcat taatcacagt ctgattgcgg taaacccgca aaaatatccg
     4981 gatacattgc ctgcacctgt tggaggtgtt caaaggtttt tttgtggcat cggtctagca
     5041 agctgtcccg aaccggttaa agtggaagaa tggatattta ctggttcgag agatcactgg
     5101 gtggcaggtc cactggcttt ggatggcaag ctctttgctc ccaatgcaga tggcagtctg
     5161 tatgtgctgg atctcagtga tggaagtctt ctggaaaaaa tcaccctgga tggagagcca
     5221 aatcaaccca accggctatg gtcgcaacca tctacagatg gcgaacgggt atttgtcaca
     5281 tcccttgatc acagcgtcta tgcaattgat attgaaactc acgacatcct ctggcacgag
     5341 gatctcggcg gcgcaattcc cggcggtgtc gtacttgcat ccgatggtat gctatatgct
     5401 ggctcacttt ccaaacagtt ggaacgtttt gacccggcca ctggtgctca tgaacccgta
     5461 ttagatacga atggttggat ctggggaacc cctgtttctg atggtgataa tttgtacttc
     5521 agtgatgtgg atgggtattt ctattcgtac aacaccgtgg accaaaaact aaattgggag
     5581 cctgttaagc cagatagtgc cattacagcc agccctctgc cccagtctgg tggtattcta
     5641 cttgcggctg agtctggcaa tgtctattca gtagacccta caggcaaggt ttccctctgg
     5701 tatgatgagc caagtgaagg caaggcttat actacgcctg tatcttccga tggacatgtt
     5761 ctgattgcat atcttgaatc agattattta ctcctcgcct tgaatgagca ggggcgtcag
     5821 gcatggacgt tcccctccca gcagtaatga ggtgttatga agaacacaac aaataaaaca
     5881 caaacagaag ttcaacgacc tgaattaccc caggggttat cacgagaatc tctgaaaacc
     5941 gtggcagtga tcgcgattct ggtgctcgct ttcctgcgat ggaatgatgt gattgccatt
     6001 ctaatcaacg taatgatatt catctatagc ctcgttgggc agaacttcgg cattgctctg
     6061 attctattta cgatattgat acgtttgatt acctggccct tgaacgcaca acaaatgaag
     6121 ggtgccgccg ccatgcaggc actgcaaaac gataaggagt ggcagggtat acagaagaag
     6181 tacgcaaaag accgcgagaa acttgcacaa gagcaaatgc gcctctaccg tgagagaggg
     6241 attaatccct ttggctcctg tctcccaacc ctggtgcaat tccccatttt gtttgcgctc
     6301 attccagcca tcacgtttgc tattggctcc acacctttaa gcatactaaa tctttcccgc
     6361 agtgtatatc cttttcagga tgtggcggct cttgtccccc tcaacagcaa gtttttatgg
     6421 atggatctgg gattgccaga acgaacttta cttttcggtc tcagtattcc cgtcctcgca
     6481 ttggtggtgg cgctaacgac atacgtacaa accaagctta ctatgccgcc gtcggcgaat
     6541 cctaatgatc aggctgccat gacgggtaaa atgatgagca tttacatgcc aattatgctt
     6601 tatttctttt ctgtcaacta tgcagccggg ctctcagttt acttcgtcat cagcaatatt
     6661 ctcagcatca ttcaatatgc aatgatgggc aaggtcaatt ggcgaaatct tttacctggc
     6721 ggacaaaaaa gtaactagga ggtgggtatg aacgagaaaa caactttgga aatcatcgct
     6781 cccacagttg aagaagccat tgctcaaggg ctggcagagt tgggcctgac cgcagatgcg
     6841 gtgagtgtag aagtgttgga cgcaggcaat aaaggcttat ttggatttgg aaaacatcag
     6901 gtgcgcgtcc gtttgacggt gaatccgcca ggcggaatgc agccatttga agcggaatca
     6961 gttccagcgt ccgtgcctga agtggagtac tccaaccaac gccctcaggc cgctcccagt
     7021 aaaccggttc ccgcggctgt gacaaaggaa tcagaatctc cgcttgagca tgatccattg
     7081 ctcgaccata cggaggctgt catttccaaa atgcttcatt tgctcaaact cgaagcacag
     7141 gtttcggctc attatggggc agcggaacgg gatggacgac ggaatatcaa tgtggatatt
     7201 cgcgggaatg atcttggggt attgatcggc cgccgctctg aaacactggc tgcatttcaa
     7261 tacgttgcct cgctgattgt cggcaaagat acacaacaat tcatacagct cacggttgat
     7321 gtcgaaggat ttcgtgagcg ccgtgagaaa caattgatac aaatggcaaa acgaatggca
     7381 gatcaggttg tcaaaagtgg gcgtcggcag accctggagc caatgccttc gggtgaacga
     7441 cgtatcattc atattgcttt gcgtgatcac ccagccgtaa aaacggaaag caccggcgat
     7501 gatccttatc gtaaagtgac catcattccg aaagattcgt aaaactcctc gtagggacac
     7561 agcaatattg tgtccctact tttttctcac ggtataattc atttatgtca aatccggttt
     7621 cattgcagcc agtcgattat ctcctcctcg gacatgtcgc gcatgatcta acaccaaccg
     7681 gacctcgact cggaggaacc gttgcgtatt ccgcactaac tgcccgtgcc atgggcatgc
     7741 gtgttggtat cgtaaccgcg gcaggaccgg aaacctccct ggaacctttg aacgaacttt
     7801 ccgtcatctc ccttccttcc acccaaagta caacgtttga aaatatttat actagcagtg
     7861 gtcgcatcca atatttgcgg gcccaggcga agcgacttga tttcagtgcc gttcctgaag
     7921 tctggcggaa ttcacccatt attcatctgg gtccgatcgc caatgagatt gaatcaagtt
     7981 tgccagagga gtttgctcca ggactactgg ggatcactcc ccagggttgg atgcgcacct
     8041 gggattcggg ttgtagggtt tcacccattg aatgggcaaa ttcggaatct gcccttgctc
     8101 aggctggtgc agtgatcatc agccgcgaag atgtaggcgg gaacgatgaa ttgattgaac
     8161 agtacgcgca gcaaacgcgt gttctggcgg taacggaagg acctgcaggt gctgttctgt
     8221 actggcatgg cgaccgccgc cggttcagag caccggacgt caatgaagtc gacgcgaccg
     8281 gcgccggcga cattttcgca gcggcatttt tcgtccgcct gcttaagacg cgtgatccgt
     8341 gggaatcgac gcgttttgca acattagttg catcacgctc agttacacgt gtcggactgg
     8401 atggaattcc cactccagag gagatcgaaa agtgtttgat ggagatcctg gactagagta
     8461 aaacgaatgt caaatcagag cccatcctta ttccgcatta tctccacaga ttattcttct
     8521 tatttatcga ttttgtttcc ggtggtattc ggatttttca ccatatattt cttcaacacg
     8581 gagaatagct cctcccagct ctttttattg atcacgactg ctgtaacggc cattggaatt
     8641 cccaccctgt ttcaaagata tagaaccatc tcttctgtat ttgcgaatgg aacgctaacc
     8701 aaaggtgtga taacccatat cggttttctg cgggggcgcg ggcgcgtgga atattcttat
     8761 actgctcaag gggagaagca tacgagcagt aatgcgatca atcgaaacgg tcgtacaagg
     8821 aatctaagaa tagggcagga agtgaaagtc gttgtagatc ccaacgatcc aaggcgcgcc
     8881 tttattcagg atatttattt gtgatcgcgg aaggtcttta ttgactcgta tctatacact
     8941 cgtcaaccaa aagggcggag tgggaaagac aacaaccgcc atcaacttgg gtgcatatct
     9001 tgcgcaatca aatcagcgcg tcctgatcgt tgatcttgac ccgcaggcaa acgccacagc
     9061 ttgcctgggg gtggataaac gtgctgtaca gtcaggtacg tacgaagcgc tacttaacgg
     9121 tgatttacct gcttcctcaa ttctaatcaa cgaacgactc aatctggcac ttctgccttc
     9181 ctctccctcc ctagccggag ccgaagtgga actagtggat gaaacgggtc gcgaattccg
     9241 tctcaaaaac gcgctgactt ctctcaatgg caagtacgac tacattctga tcgattgccc
     9301 gccctccctt gggctcctga cagtcaatgg gctggtggcg gctcgggatg gtgtgcttgt
     9361 tcctgtacaa tgtgaatatc tcgcgttgga aggattggga cagatcaccc agacaattga
     9421 gcgagtccag gctgcgctct ttccgggctt gcgggtacgc ggcgtaatac tgacgatgtt
     9481 cgacccgcgc accaggctct ctgccgatgt agtgaaagaa gtcaataacc actttccggg
     9541 tcaggtcttt aaaagtattg tgccgcgcag tattcgcctc gcagaggcac cctcgtac
//
LOCUS       DASTJP010000183         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1084360, whole genome shotgun sequence.
ACCESSION   DASTJP010000183 DASTJP010000000
VERSION     DASTJP010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084360"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFQ23_05555"
     CDS             <1..506
                     /locus_tag="VFQ23_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906080.1"
                     /translation="PHYLVHIMPDSWVRPQTWNPLGELVLKSDSEEAQIVEIGGQDAG
                     REMRSCQVELPPRKRTRVKDEFFSLSYTHMKSMPNARIGIHSPIRRETYVIDRTDWGN
                     IWVYGLEILLTGYLTREAFNRRASHIQAGARVFQYDKTRTKSLAVPVSDLKPLSELVE
                     RLKASTL"
     gene            599..1981
                     /locus_tag="VFQ23_05560"
     CDS             599..1981
                     /locus_tag="VFQ23_05560"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9906081.1"
                     /translation="MKLKRIGFGFLILSLLSCNYVTQMVFPPTATPLPTATLLPTTTP
                     IPTPTALVPAYVPPECASTPLATMSPDTVLPTLESETNQDISQSDQLQIVQELSDIVE
                     EVYVYPDYNGKDWNEIQSRYRAKVETGLDTPTFYLEMQSMIEELQDEHSFFLSPLQAD
                     ASEAELKGESEYVGIGIYYVLDAEQKHLVVVSTFPGSPAEYAGIGPHDSILLADGLPI
                     DVETGNRLRGPTCSAVVLTVQSPGEAPREVMLLRQEISAELDIEARLVPTNDGSKIGY
                     IFIPSFFDETIPPEIEDALNEFGQLDGLILDVRMNGGGSSSVVNPILEHFADGRLGEF
                     VSRSDSRPMDVDPNPIHNSQTVPLVVLVSEDTVSFGEIFAGVMRDSRGAKIVGETSLG
                     NVEVLHGYDFDDGSQVWIASETFFPENSDDNWEETGIVPDVQAFAEWETFYFETDPSV
                     AAAVQLLGHQ"
     gene            1984..2409
                     /locus_tag="VFQ23_05565"
     CDS             1984..2409
                     /locus_tag="VFQ23_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906082.1"
                     /translation="MEFPFEYRTLGKFTYNVLTDPPIIKAYLLKWIMKEWQSDHNEAP
                     DEHWTVEWMTVFPKMEFSLEVVRLEDIHPNADLFSVEEFQSGLRERADEREESMLRGV
                     SIEALLVNRSGFELMDGYTRYTVLIRHQQKEVYAYVGTV"
     gene            2596..3834
                     /locus_tag="VFQ23_05570"
     CDS             2596..3834
                     /locus_tag="VFQ23_05570"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET9906083.1"
                     /translation="MSKRNIVFIGVGFLTLIILALQIPGINSRVAWRYEVARTYIRNV
                     INPVGSAPTAIPNTPLPATSLPSPTSPVTSTIEVIATAIPPTATLAPPPPQAMLGSPP
                     YEKQTANNCGPAALSMMLHMVGWSGSQKDISDIIKPVNGDRNVNPEEMAFWVRNYAGW
                     LRIEYRVGGDIETLQRLISSGFPVIVEGTTTLNPDDTGWPDDDLWAAHYLLLTGYDDT
                     AQTFVAQDPYRGPDKTITYEQLESEWKPFNYVYMVVYLPEQEEQIKTLLASNWDATLN
                     RQNALARAEAAIIEDPTDAFAWFNVGSNLVYFERYFEANDAYDKARELGLPQRMFRYQ
                     FGPFIANFHAQRTEDLLALTDYALQRTDMSEETWLWHGWALYRQGNTNGAVEDWRKAL
                     SIHPGYEDALYALNFVGAAP"
BASE COUNT          984 a          967 c          990 g          921 t
ORIGIN      
        1 aaccacatta tttagttcac attatgcccg attcatgggt gcggccgcaa acgtggaatc
       61 cgcttgggga actggtgcta aagtctgact cagaggaggc gcaaatcgtc gaaatcggcg
      121 ggcaggacgc ggggcgcgag atgcgatcct gccaggtgga actcccaccg cgaaaacgaa
      181 cccgggtcaa agatgaattc ttttcgttat cctacacaca catgaagtct atgccgaatg
      241 cgcgtattgg catccatagc ccgattcgca gagagaccta cgttattgat cgaacagatt
      301 gggggaatat ctgggtctat ggcctggaaa ttttgctgac cggatatcta acgcgcgagg
      361 cgttcaaccg ccgggcaagt cacattcagg caggtgcacg cgtgtttcag tatgataaaa
      421 ctcgtacgaa aagcctggcc gtccccgtct ctgatctcaa accgctctcc gaattagtcg
      481 agcgcctgaa agcatcgacc ctgtaacacg ttctgcctgc gcgccgtcat gaacaaaact
      541 gcgaaacccc ttgcgaaaca acgggtggtt aacgcgaagg catagtaaaa ggttgattat
      601 gaaattaaaa cgaataggtt ttggattttt gatcttatcc ctgctgtcct gcaattatgt
      661 gacacagatg gtgtttcctc caaccgcgac tcccctcccc acggctactc tactaccgac
      721 gactacaccg atcccgacgc ccactgccct cgtcccggct tatgtgccac cggaatgcgc
      781 atccacgccg ctggcaacca tgtcaccgga caccgtgctg cctaccctgg agagcgaaac
      841 aaatcaggac atttcacaaa gtgaccaact gcagatagtg caagagcttt ccgatatcgt
      901 tgaagaggtc tatgtctacc ccgactacaa cggcaaggac tggaacgaga tccaatccag
      961 atatcgagcg aaggtcgaga ccgggctgga tacccccact ttctatctcg aaatgcagtc
     1021 gatgatcgaa gagctacagg atgaacactc gtttttcctc tcacccctgc aagctgatgc
     1081 atcagaagca gaattgaaag gagaatcgga gtacgttggt attggcattt attatgttct
     1141 ggatgccgaa cagaaacatc tggtggtcgt ttccaccttc cccggttccc ccgcagaata
     1201 tgcgggcatc ggacctcatg acagcatttt gcttgcggat ggccttccca ttgatgtgga
     1261 aacaggcaac cggctgcgcg gaccgacatg ttcggcggtc gtgttgaccg tgcaatcgcc
     1321 gggggaggca cctcgagagg taatgctcct gcgtcaggag attagcgctg aactcgatat
     1381 tgaagcacgg ctagtcccaa ccaacgacgg atcgaagatc ggttatattt tcatcccttc
     1441 attctttgat gagaccatcc cacctgaaat cgaagacgcc ttgaacgagt ttggtcaact
     1501 ggatggcttg attctggatg tgcgcatgaa tggaggcgga agtagttccg tagtgaatcc
     1561 cattctggaa cattttgccg atggaaggct tggagaattc gtcagccgct ccgattcccg
     1621 ccccatggat gtggatccta atccaatcca taattcgcag acggttccac tcgtggtgct
     1681 ggtcagcgaa gatacagtca gcttcggtga gatcttcgcc ggagtaatga gagattcaag
     1741 aggtgcaaaa atcgtcggtg aaacaagcct tggtaacgta gaagtccttc atggttatga
     1801 ttttgacgac gggtcacagg tttggattgc ctccgaaacg ttctttccgg aaaattccga
     1861 tgacaactgg gaagagaccg gtattgttcc cgatgtgcaa gcctttgcgg agtgggaaac
     1921 gttttatttc gagaccgatc catctgttgc tgcggcggtt cagttgcttg gacatcagta
     1981 aggatggaat tccctttcga atatcgcacg cttgggaagt tcacgtataa cgtcctgacg
     2041 gacccgccca tcatcaaagc ttacttattg aagtggatca tgaaagagtg gcagtctgac
     2101 cacaatgaag cacctgatga acactggacc gttgagtgga tgacggtatt ccctaaaatg
     2161 gagttctcac tggaagttgt gcggctggag gatattcatc cgaacgcaga tttgttcagt
     2221 gtggaagagt ttcaaagtgg acttagagaa cgcgccgatg aacgcgaaga atctatgctg
     2281 cgtggcgttt ccatcgaggc gcttctggtc aatcgtagcg gtttcgaatt aatggacgga
     2341 tatacaagat acactgtgtt gatccgccac cagcaaaaag aagtttatgc gtatgtgggg
     2401 actgtctaaa aaacatcaaa atcatcagca agacttttaa cgcaaagtcg cacgcttgcc
     2461 ctggcgggca gtgccaggga ggacgcaagg tctaattctt cgcgtcgtta cattagattc
     2521 catctgtgaa atgcggataa ggtgggggat acatgaatta atttcgactc atactcctct
     2581 ggtacacttt tcggcatgtc gaaaagaaac atcgtgttta ttggggttgg ttttctgact
     2641 ctgataatct tggcattaca gatcccaggt atcaacagtc gagttgcctg gcggtatgaa
     2701 gttgcaagga catatataag gaatgtgatc aatccagtgg gcagtgcacc aactgcaatt
     2761 ccaaacacgc ccctgcctgc aacaagtttg cccagtccca caagcccagt aacttccacg
     2821 attgaagtta ttgcaacagc gattccccca acagccaccc tggctccccc gcctccgcag
     2881 gcgatgctgg gttcccctcc ttacgaaaaa caaactgcga ataattgcgg acccgccgca
     2941 ctttcgatga tgctgcacat ggtcggctgg agcggcagtc agaaagacat ctcggatatt
     3001 atcaaacctg tcaatggcga ccgcaacgtc aacccggaag agatggcatt ttgggtacga
     3061 aattatgcag gttggcttcg cattgaatac cgtgtgggtg gcgatatcga aacactccaa
     3121 cgattgatct cctcaggttt ccccgtgatt gtcgaaggga caacgacgtt gaatcctgac
     3181 gacactggct ggccagatga tgacctctgg gcagcacatt acctcctcct gacgggatac
     3241 gacgatacag cccagacttt cgtcgcacaa gatccttatc gcggaccaga taagacgatc
     3301 acatatgaac aattggaatc tgaatggaag ccgttcaatt atgtgtatat ggttgtttat
     3361 ctcccggaac aggaagagca gatcaaaacc ctgctcgcct ccaattggga cgcaaccttg
     3421 aaccggcaaa acgcgttagc ccgagccgaa gccgctatca ttgaagaccc cacagacgcg
     3481 ttcgcgtggt tcaacgttgg cagcaatctg gtttattttg agcgctactt cgaagccaac
     3541 gatgcttatg acaaggcacg cgaattaggt ttgccacagc gcatgttccg ttatcagttt
     3601 ggtccgttca ttgctaattt ccatgcccag cgtacagaag atttactggc actgactgat
     3661 tacgcattgc agcgaaccga catgtctgaa gaaacatggc tctggcatgg ctgggcttta
     3721 tatcgccagg gtaatacgaa tggagcggtg gaagattggc gcaaggcctt gtcaattcac
     3781 ccgggctatg aagatgcctt atatgcgctt aactttgtgg gtgcagcacc ataacgacat
     3841 gagcttgctt tgggccggac gt
//
LOCUS       DASTJP010000184         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_928639, whole genome shotgun sequence.
ACCESSION   DASTJP010000184 DASTJP010000000
VERSION     DASTJP010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928639"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFQ23_05575"
     CDS             complement(<1..911)
                     /locus_tag="VFQ23_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906084.1"
                     /translation="MDLSIVFPMFWGRKKEKVVKDGDYFYDHPTALDDEGTIPPLFES
                     MNVLKDREFDVIAVAGANHPSGAEKVERQANKLLNKYAKKAKVKLHYFSYSHLARLHD
                     YLRELGREDLVETISLVGYSPLRNACLVAAHILGKEIAVSIDDDCLFIEPDYIGRIKS
                     KMLSDFEGKPIYAYCGPYITAKDAIYLDIPTAPHAVYWNVIEVMNEAFRKYIVESPGM
                     MESPFAIMGNIAVHRDFFTRVPLDPPMQRGEDMDWVMNAHICGERFVMDKDLIIKHDP
                     PPRPYPTWRPLREDIYRFRYQQAKIAN"
     gene            1096..1770
                     /locus_tag="VFQ23_05580"
     CDS             1096..1770
                     /locus_tag="VFQ23_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906085.1"
                     /translation="MKRKLFLALYALLLTMCRTYSQVLPVSSVDELSCQPYSNYFEGT
                     QGLECTYRCPNGEKTGPRDFETDPSLSATKGDLDRLFCDFVLPTSTQAATVVAGSSTP
                     AETPTVEATATTAATATIAPTRLPLFTGQVTMCDTGGDLISFRMVDPPPDLTNTILRV
                     EIAGQESSCTVNPTNPSLMTCTLPDDLAFPASVIVRLDDVVVNELTLDGTGCDQITTP
                     VATTSP"
     gene            complement(1793..>2036)
                     /locus_tag="VFQ23_05585"
     CDS             complement(1793..>2036)
                     /locus_tag="VFQ23_05585"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET9906086.1"
                     /translation="LAGGKYESDWVKNLLKKPSVAVRIAQHNFKGIARIIREQNEEMM
                     ARTALAEKYQEWDEGRTLSQWARTALPVAIDIHSKK"
BASE COUNT          491 a          530 c          488 g          527 t
ORIGIN      
        1 ctattggcga tctttgcctg ctggtagcga aaccgataga tgtcctcacg taatggtcgc
       61 caggtgggat agggacgcgg cggcggatca tgcttgatga ttaggtcttt atccatcacg
      121 aagcgctcgc cgcagatatg agcattcatc acccagtcca tgtcttcgcc gcgctgcatt
      181 ggagggtcga gcgggacgcg ggtgaagaag tcccgatgca cagcgatatt gcccatgata
      241 gcaaacgggg attccatcat gcctggtgat tccacaatat acttcctgaa cgcctcgttc
      301 atcacctcga tcacgttcca atacacggcg tgaggcgccg tgggtatatc cagatagatt
      361 gcgtctttcg ctgtgatata gggtccgcaa taggcgtaga tcggtttccc ctcaaaatcc
      421 gaaagcattt tcgatttgat gcgtccgata tagtccggct cgatgaacag acaatcatca
      481 tcaatggaga cagcgatctc tttgccgaga atgtgcgcgg cgaccagaca tgcattccgc
      541 agaggtgaat aaccgacaag tgaaatggtt tcgacgagat cctctctgcc caattcacgc
      601 agataatcat gcaagcgtgc gagatgtgaa taactgaaat aatgaagttt taccttggcc
      661 tttttggcat acttattgag caacttgttt gcctgcctct ccaccttttc tgccccggat
      721 ggatggttgg cacccgccac cgcaatgaca tcaaactcgc gatctttcaa cacattcata
      781 ctttcgaaga ggggtgggat tgtcccttca tcatccaacg cggtcgggtg atcgtaaaaa
      841 taatccccgt ccttgacgac tttctccttt tttctgcccc agaacatggg aaacacaatc
      901 gagagatcca tgccagcctc cgtattcaaa tagagatgcg tccgaaagaa agagtcaggt
      961 aaagtatatc tcggtccgac atagcctcca agtattgtac acgctgcagg cacgttgcaa
     1021 acggtgagga gtttcgttga ttaacagtgt gaatatcaca ttaattaatt cagatttctt
     1081 tgaagtagag gacgcatgaa aagaaaattg ttcctggcgc tctatgcttt gttattgacc
     1141 atgtgtcgga cgtatagtca ggtcttaccc gttagttccg tagacgaact gagctgccag
     1201 ccctacagta attattttga aggtactcag gggcttgagt gtacttatcg atgtccgaac
     1261 ggtgaaaaga caggtccccg cgattttgaa accgacccat ccctctctgc cacaaaaggc
     1321 gacctcgacc gtctgttctg tgactttgtc cttcccacgt ctactcaggc cgcgacggtt
     1381 gttgcaggtt cttcaacgcc tgctgaaact ccgacggtag aggctactgc tactacagct
     1441 gcaactgcga cgattgcacc cacaaggtta cccctgttta ctggtcaggt gacaatgtgc
     1501 gataccggtg gggatttgat cagtttcagg atggtcgacc ctccgcccga tttgaccaat
     1561 acaatactac gtgtggaaat cgctggtcag gaaagcagct gcaccgtcaa cccaaccaac
     1621 ccatctctga tgacctgtac gctgcctgat gatctggcct tccctgccag cgtgattgtc
     1681 cgtttggatg atgttgtagt gaacgaactg actctggatg gaacaggttg tgaccaaatt
     1741 acaacacctg tagcaacaac ctctccctaa ctggaaccgg caccgcgaaa aattatttct
     1801 ttgaatgaat atcaatcgcg acgggtaaag cggtccgcgc ccattgactg agagtccgcc
     1861 cttcgtccca ttcctgatat ttctccgcga gcgcggtacg cgccatcatt tcttcatttt
     1921 gttcgcgtat gatgcgggct atacccttaa agttatgttg tgcaatccgc actgcgacag
     1981 agggtttctt gagcaggttc ttaacccaat ccgattcata ctttccccca gccagg
//
LOCUS       DASTJP010000185        17601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_28522, whole genome shotgun sequence.
ACCESSION   DASTJP010000185 DASTJP010000000
VERSION     DASTJP010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28522"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VFQ23_05590"
     CDS             <1..1303
                     /locus_tag="VFQ23_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET9906087.1"
                     /translation="PITIALLIVALLLVGAGISAVIFFSFLGGFSTNSPFDVRNISSE
                     VEESKTLQVDSGKPLTLKVVDAAGEITVTGADVEEVEVRVVKTAYDSTQARADEEVKG
                     VKYSIEQSGNIITLTYELPKSMNFSNNVNTVDFIVTVPIQTSVDAETSFGDIRVDNLE
                     GSVAVQNDFGEVSVLNITGAVSVSSNGGTLTATSIKAGTGDIELHSEFGSITLEKANG
                     RNITLDTNSGSINLNEVRATSDLTANTQFGDVTFENGSAASVSIENNGGAVSLLKLNI
                     KDQIKVQDQFGNIKLDQALSSSYDLHTNSGAITIDGAEGDLKAHTDFGNIEIANAESV
                     TLDVKTNSGSILFSGLLGVGPHNIKTDFGGIDLSLSGDAKLDVDLKTDFGSISSDIPI
                     TVTLNGDANSNGGEIIGIINGGGDQLTVQTSSGSINIKAVK"
     gene            1357..1692
                     /locus_tag="VFQ23_05595"
     CDS             1357..1692
                     /locus_tag="VFQ23_05595"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HET9906088.1"
                     /translation="MDAFVTCFGTIGVLAIIFGFVAFLRYMNYKETLALAEKGLTRPE
                     SKNAKGLLRWGVVITALGIALTLGLYPIGFFAGSSYPLHLGPWMLGGFVPVFLGLALI
                     LLHYLTERN"
     gene            1698..2327
                     /locus_tag="VFQ23_05600"
     CDS             1698..2327
                     /locus_tag="VFQ23_05600"
                     /inference="COORDINATES: protein motif:HMM:NF017069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906089.1"
                     /translation="MTIQNAYNQWSDSYDTDGNLTRDLDQIVTRSALKNLHFHSILEL
                     GCGTGKNTSFLSQIGDSLYAVDFSQGMIDKAREKVKAESVKFSVMDISQKWVFDDHSF
                     DLISCNLVLEHIKDLSFVFSEAARVLQHKGRFFINELHPFRQYAGKKARFDREGETIE
                     VEAFVHHISDFVNGAMNNGLKLIKLDEYWHETDQNKPPRIVSFSFEKQN"
     gene            2481..4364
                     /gene="htpG"
                     /locus_tag="VFQ23_05605"
     CDS             2481..4364
                     /gene="htpG"
                     /locus_tag="VFQ23_05605"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET9906090.1"
                     /translation="MTDSNPTATSENTQEFSFKAETKQLLNILIHSLYKDREVFLREL
                     LSNASDALNRMRFEMVTNQNVVDPKVDLKIHIKADKDARLLTIQDTGIGMTRDEIIEN
                     LGTIAQSGARKFLEATKDQQVDFAQVIGQFGVGFYSVFMVADWVRVTSRSYKPDAEAV
                     SWYATGEDNYQISTAEMSERGTKIEIKLKDDAAEFADDFRLRNIISKHSDYIGFPIYI
                     GDGNAPVNKQTSLWRTSRQEVKEEQYKEFYKQTTLDFEDPMLHIHMITDAPVQLYALL
                     YVPGKAERGIFALRKDDGLKLYSRNILIDEYNKDLLPEYLRFMQGVVDSEDLPLNVSR
                     ETVQSSGLMPKLKKVLTNQVIKDLETLAKNNAEKYQSFWQEFGGYIKQGLAGETADGE
                     KLHSLLRFKTNLNPETWSSLEEYASRMKEGQNIIYYIVGDDPKSVLRSPHLDYFHTQG
                     TEVLLLTEPMDSFMLMGLRKYKDLELQNVAQAEIDTTETPKDEAQAEKIPDPDFNVLV
                     ERFKQVLGDRVTDVRASNRLSQSVARLADPDGTLNPELQRVYRYLGKEYEAPKKILEL
                     NPANSILKGLLTLESGSELQNAIIEQIYDSALLVEGLHPDPSSIAPRVQQIIEAALKQ
                     NSR"
     gene            complement(4443..5327)
                     /locus_tag="VFQ23_05610"
     CDS             complement(4443..5327)
                     /locus_tag="VFQ23_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906091.1"
                     /translation="MTTTTRTIQKAKSRLPLHGWIGFGLILIFWILNWALTGARTHWG
                     FFPLWLGYCLAVDGLVWWRTGTSLLTRSPRRYLGLFLVSAPVWWFFELLNLRTQNWTY
                     IGSEIFSPLEYAFWTTLSFTTVIPAVFGSAELFASFDFVRRLKRGPVIGTGIRTILFF
                     FILGWIMLALLLIWPRIFFGFIWLSPYFILEPINIWLGNRSLAYWTREGDWRPVIALW
                     LGVLMTAFFWEMWNYFSYPKWIYDVPWGDWLHIFEMPLLGYGGYLPFALELYALYHWT
                     AGLLGDKTTDYVKVISEA"
     gene            5463..5846
                     /locus_tag="VFQ23_05615"
     CDS             5463..5846
                     /locus_tag="VFQ23_05615"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HET9906092.1"
                     /translation="MPLIPQTCPSCSSPLVVTQLNCTACGTGVVGKFELSPFFRLSPE
                     SLKFLEVFVRNRGNVKEMERETGESYWAIRRQLDEVITEMGMEAPQEDDLSTRRQEIL
                     ARLSRGEINVQEATKSLSQLKGDKS"
     gene            5843..7147
                     /locus_tag="VFQ23_05620"
     CDS             5843..7147
                     /locus_tag="VFQ23_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906093.1"
                     /translation="MNGQKERLMVLDMIAEGKITAEEAEQLFKAMDEVGDKLDSDSPE
                     LVSPLSHLSHLSSLNPPSPLPGSSRSKELVAALKEAGIDHVTLSDVQEMQTHRLTAEY
                     IREMLALGVEPEGLGEWINLRVHDITPRYVRELREMGMTDFDVDDLVELRIHGVSGKY
                     AAALHGIGLKGFGADQLVELSQNDVSVKYIAELREVGLKDLDVDELIELSQNDVSARY
                     IADLRQAGLKDLDVDDLVELSQHDVSAKLIVALGQQGLKSLDIDELVELSNHDVSPEF
                     IAEIRQLGFTDLSVDELVELSNHDVSPEFIAELREMGLKDFDIDEVIELRNHDVSANF
                     IRSLRDVGYTGFDIDDLVELRIHNVTAKSVRELHEMGFTNLPVDELVELNIHHVTPRF
                     IREMRQKYGEDLPLSKIIEMRIHGVDEDLLEELRAAGVKIRR"
     gene            complement(7212..8351)
                     /locus_tag="VFQ23_05625"
     CDS             complement(7212..8351)
                     /locus_tag="VFQ23_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906094.1"
                     /translation="MRLTVWKIAVFMGLFSMLALTFSVYSQLEVPAPEGPYAVGRTTI
                     NWMDASRPEVLTENPNDFREVAAMIWYPAEAGTGVKAGYFPLLSLVSDALLQSGEVEW
                     WQVLGLQFIRSEIPFDAEPLRNQIPFPVVMLSPGNGTNMEFYSSLASEIASHGYIVIG
                     INHPYDVPAVELSNGDVAPYDKDQWSLDMTAHQRYTRERIKVRTADILFVLEQLDQMN
                     STGRFAGRMDLDSIAVAGHSLGGITASESCKADARFKACLNYDGLQNGGPFSMEETAI
                     PPEQPFMFLTRESQLHPKLIQSFESTSESYWVVVHGASHESFTDGPLLQPSLLPGPNQ
                     ADELMSLIEEYSLAFLNHTLKGQPSQLLSGTVDGEAVSLKVFPAN"
     gene            complement(8449..9582)
                     /locus_tag="VFQ23_05630"
     CDS             complement(8449..9582)
                     /locus_tag="VFQ23_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HET9906095.1"
                     /translation="MDKLEQLLSELVSINSINPDLVPGSPGEGEIAHHIADWLTSADV
                     EVELVESVSGRPNVVGIARGTGGGKTLLLNGHMDTVGITGMTDAHKPIIKDGRLYGRG
                     AYDMKCGVAACMVAIAEAKKHALSGDVIFTAVIDEEYASLGTMDLADRYHAHAAIIAE
                     PTELQLIVAHRGFVWLDVETIGKAAHGSRPDLGLDAIARMGKVLVEIERLDQKLRSNP
                     THPLLGSGSLHASLITGGQELSSYPERCVLSVERRTLPGETPEAVEAEFIKIIEEIQQ
                     SDSTFNAVVRRGIDRSPLETSEESEIVRVLQETCDTALGQPAQIAGVQYWTDAAVLAA
                     AGIPSVLFGPSGAGAHAVEEWVDLPSVRTCSEIYLATALNFCK"
     gene            complement(9596..10333)
                     /locus_tag="VFQ23_05635"
     CDS             complement(9596..10333)
                     /locus_tag="VFQ23_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906096.1"
                     /translation="MFSFFKKKDQDEQPNSQGGISPVLLDLRKTLYTNESLDPLLARI
                     QVDAKSVFPWSNFVAANQALKENNKDKALTQLKEIVATPQGLETRIRLQAWHTLVALG
                     ELPIDTLRGYTQGVVMENHTVKGLDVVAGYSDNSARYWNNAGTGVIWDARDPEIDKLV
                     FNLLSVGFEITKRIGVGLRDTPPVPEKGFVRFFIMAFDGSTVGEGRYDQLSKDPMGRV
                     AIDAGLKLMSALMKKQKDKNQMMRYSS"
     gene            complement(10365..12722)
                     /locus_tag="VFQ23_05640"
     CDS             complement(10365..12722)
                     /locus_tag="VFQ23_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9906097.1"
                     /translation="MSVRRTKILRDLLVNKSRSLLVILAVTVGVAAFGLMITGRVVLE
                     ENLRDGYAATQPAHTILSLSAFDDDLLKHVQELDFILSAQARRLDQARVLSGSDTWLG
                     FELQTLPDFNSILINKLTLEGEASLPPAVNGILIERSLKNIMEVGDKIEIQLLNGDEY
                     TLAVSGYVNDLSHLPSEISLSGLGYISLDTADELGFDNHYNQLLVVFANAVTRNEIEI
                     QTTKLIDDLEDAGYPVFNASVPIPNQYALGGNMTSVLFILNALGVLTLILSAFLVTSV
                     MSAIMSQQIPQIGILKSLGGRIHQTMALYFQEVLLFGILALLLAIPMGLVGAYFLADG
                     VATGMNFNVEHFSLPTITLVLQAFSALLAPLLASLFPIVSGSLITIREAISNYKPETG
                     TRFGSVRLFGELPQLVNLSVRNTFRRKGRLALTFVALLLAGAMFIAIIGIRQSMREAL
                     KEMQGDLNYDVGIDFVQPYSMRKIESETLELDGVRAVETWSVDSGRLVFNDDHLSGSI
                     ILYGVPEETRMAQPEVIHGSWLSSTTTRGIYVNADFLDLSPDLKVGSVITLNIAGREE
                     NWIILGSGGRGFVPVAYMFYDELADETGLDGFANRLVIQTTRSDPAFQSALQSDVLTQ
                     LDAINFDVLGSQTTTQLKETNAAQMDILIVLLLAMVVLIALVGGLGLAITMSLNVIER
                     TREIGILRSLGAQNGVVRRVVIVEGLVIGLISWAVSIPCSIPLAIWLGDSLGISLLAR
                     PLDYIFSVPAMLMWLGLMTIISVIASIIPAQSAARLTIRDALVYE"
     gene            complement(12752..14389)
                     /locus_tag="VFQ23_05645"
     CDS             complement(12752..14389)
                     /locus_tag="VFQ23_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9906098.1"
                     /translation="MQKYNNTGKGICLKPVAFLVDPYNLSRPDEEVSENVTSSKLGEM
                     ASNPAPQPLIEIKNLRKVYQTPAGDFTAVNGINVEVQRGEFVAIIGKSGSGKSTFINM
                     ITGIDRPTSGEILINGAPVHSFNEGQMAAWRGRNLGIVFQFFQLFPTLTILENIILPM
                     ELNNLYSKRERKERALHLLEKVEMTAQAHKLPSAISGGQQQRVAIARALANDPPLLIA
                     DEPTGNLDSKTADKIFSLFENLVAGGTTILMVTHDSDLARRVNRTILISDGEVVNEYL
                     VRALSSLTQDQLVEISRTINPQIFQPNSAIVRQGEKGDKFYILLNGKADVYLNMPGGS
                     ELFVNQLKPGQYFGEMALLGGGVRRATVKASQDGPASVAALDENAFNTLIDESRTLRE
                     ELMGLVERRNTYHHLQTLSSLDENVLSSILKNTEPKIYLEGQEVIKQGDVGDTFYLLL
                     DGQVDILVRDERGSEVLVNQLSRGSYFGEMALMGNKRRNATVRVSQGHSAKLIELGVR
                     EFDQLEDSSEIFKTHIYGAAGTRKQQLEDTRKQQASA"
     regulatory      14429..14520
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            14641..15009
                     /locus_tag="VFQ23_05650"
     CDS             14641..15009
                     /locus_tag="VFQ23_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906099.1"
                     /translation="MLRNMKVLFVLLVAMVLAVSAYAFAAANTVPTTKAGTGAGAISG
                     YTVSNVVYNLNTSDPTQLDSVDLTLDAAATNVQIKLVAAGSTWYDCTNGSGNNWSCDT
                     TSPAQSVASMDELEVVANSN"
     gene            15110..16807
                     /locus_tag="VFQ23_05655"
     CDS             15110..16807
                     /locus_tag="VFQ23_05655"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF036452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9906100.1"
                     /translation="MKKHSNQALPGFLISLFVAVMFWLVLAPTQLGGQVTYVIVDGNS
                     MEPKFHFGDLVLVRKQSTYQIGDAVTYQNAELGRLVFHRIVDIQTDHFILQGDHNTWL
                     DGYQPNQSEIIGKLWVHIPVLGKAIQWAREPLHLAIGCGLFGGIMAAGIILNPTGQNK
                     RKITKSGGYGGLLDVGMYLTGFFALALLGLTIFAFTRPLSRPVENIQYQQDGNFTYSA
                     AGTPDVYDTATIRPGEPIFPKLTCFLNVGMVYNFTGGGVQQLAGNHQFYARVFDEKSG
                     WQRTLPLQTQTAFEGNSYSSRADLDLCQIEAMVTSVEEQTGLHPNIYTMQVISETTIS
                     GTLDNSPIRDTLDSRLTFKFDEVHFYLEESSTQEDPLHTTKSGMIITSDQRPNTLSIA
                     GREISIQNLRMLSLLGLAISVLSLLPLSWFFFNKKLNGQADMIRLKYRHLLMDVHRIN
                     FQQDLPVVDVASIDDLVKLAERQSSMIMHMDVNYLHYYTVQSNGVTYRYVISSRPGQS
                     RRSQTLPRLDAPANPLRQYMEAAPLDNTNYGYRVDLNQNEYAVPDEYQTIFLNKIKL"
     gene            16804..17199
                     /locus_tag="VFQ23_05660"
     CDS             16804..17199
                     /locus_tag="VFQ23_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906101.1"
                     /translation="MKTSFLMRFFWLSIFSVLLFSVVTAVAATNTIPNTRLGYKNLAL
                     FHNNLKPSACASISVTTLVTGAGVITGTMGNDLILASPGVDIIDGSGGNDCIVGGSSD
                     DILTGGLGNDICLGGPGTDSFISCEGQDQ"
BASE COUNT         4677 a         4408 c         4246 g         4270 t
ORIGIN      
        1 accgatcaca attgcattat tgattgtagc actcctgctt gtgggtgcag ggatcagcgc
       61 agtcatattt tttagttttc tgggagggtt ttctaccaat agccccttcg atgtacggaa
      121 tatttcttca gaagtagaag aaagcaagac gcttcaggtg gactcaggga aaccgcttac
      181 gctgaaagta gttgacgcag ccggcgagat caccgtgacc ggtgcagatg tggaggaggt
      241 ggaagtcagg gtggttaaga ctgcatatga ctctacccaa gcccgagcgg atgaagaagt
      301 aaagggcgtc aaatacagca tcgaacagag tggcaacatc attaccctca cctacgaatt
      361 acccaagtcg atgaacttta gtaacaatgt caacacagtg gattttattg tcaccgtccc
      421 gatccaaacc agcgtggatg cggaaaccag ttttggtgat atccgtgtgg acaatcttga
      481 gggcagtgtg gcggttcaga acgatttcgg cgaagtaagc gtcctcaaca tcaccggggc
      541 cgtatcggtg tcatcgaacg gcgggacttt aactgccact tctatcaaag ccggcaccgg
      601 agatattgaa ctgcactccg aattcggaag catcaccctc gaaaaagcga atggcaggaa
      661 tatcacactg gatacaaaca gcggttccat caatttgaac gaagttcgag caaccagcga
      721 tctgactgcc aacactcagt ttggagatgt tacttttgaa aacggctcgg cggcctcggt
      781 cagcatcgaa aacaatggcg gcgccgtatc gctcctcaaa ctaaacatta aagatcaaat
      841 caaggtgcag gatcagttcg gcaatatcaa attagatcaa gcactttctt cctcctatga
      901 cctgcataca aacagtggag ccatcaccat tgatggcgcg gaaggcgatc tcaaagctca
      961 taccgatttt ggtaacatcg aaattgcgaa tgcggaatct gtcaccctgg atgtgaaaac
     1021 gaacagcggc tccattctct ttagtggctt attaggtgtg ggtcctcaca atatcaaaac
     1081 tgattttggt ggcattgatc tcagcttgtc tggcgatgca aaactcgatg tagacctcaa
     1141 aaccgatttt gggagtatta gttcagatat ccctattact gtcacattaa atggtgatgc
     1201 aaattccaat ggtggagaaa tcatcggcat tatcaacgga ggtggtgacc aactcaccgt
     1261 tcaaacgagc agtggctcga taaatatcaa ggcagtcaag taaaataaca gggcttggag
     1321 aaattccaag ccctgaattt ttcacgagga ttgcatatgg acgccttcgt tacgtgtttc
     1381 ggcaccattg gggtgcttgc tatcattttt ggattcgtgg cttttctgcg ttacatgaat
     1441 tacaaagaaa cgctcgcgct ggctgaaaaa ggattgacac gcccagagtc aaaaaacgcc
     1501 aagggacttt tgcgctgggg ggttgtcatt acagcgctgg gtattgcatt aacactggga
     1561 ctctacccta tcggattctt tgccggaagc agttacccac ttcatctcgg accctggatg
     1621 ctgggaggct ttgttccagt ttttctgggc ctggcgttga tcctgcttca ttacctgacg
     1681 gaaagaaatt gataccaatg accattcaaa acgcgtataa ccagtggtct gattcctatg
     1741 atacggacgg gaatctgacg cgcgatctag atcaaattgt gactcgatcc gcacttaaaa
     1801 acctacattt tcattccatt cttgagcttg gatgcggaac gggaaagaat acttcgtttc
     1861 tctcacagat cggggatagc ctgtatgccg tagatttttc gcagggaatg atcgacaaag
     1921 ccagggaaaa agtcaaagca gaaagcgtca aattctccgt gatggatatt tctcaaaagt
     1981 gggtatttga cgatcactcg tttgatctca tcagctgtaa tctcgtcctg gagcatatta
     2041 aagacctgtc cttcgttttc tcagaagcag cgcgagtctt gcagcacaaa ggacgatttt
     2101 tcatcaatga gctgcatcca tttcgacaat atgcgggaaa aaaagcaagg tttgacagag
     2161 aaggcgaaac gatcgaagtg gaggcgttcg tgcaccacat ttcggacttc gtaaatgggg
     2221 cgatgaacaa cggattaaag ttgataaaat tggatgaata ttggcacgaa acagatcaaa
     2281 acaagccgcc cagaattgtt tcattctcgt ttgagaaaca aaattaagct tgtagataat
     2341 tttctaacaa aactcttgcc gcctgttgac gagtgcgata taaaatatca tccagagcaa
     2401 gttatttttt aaatcctcat agtttcacca tttcacaccc tgacagatcg gggtatccag
     2461 gtttcttaca ggaggtagct atgaccgact caaacccaac tgcaacttct gaaaacacgc
     2521 aggaattttc gtttaaagcc gagacgaagc agcttctcaa tattctgatc cattcgttat
     2581 acaaggatcg cgaagtcttc ctgcgtgaat tactctctaa cgcgtccgac gcactcaacc
     2641 gcatgcgctt tgagatggtc accaatcaaa atgtggttga ccccaaagtg gatttgaaaa
     2701 tccacatcaa agcggataag gacgcccgcc tgctcacaat tcaggacaca ggcatcggca
     2761 tgactcggga tgaaatcatc gaaaaccttg gcaccattgc ccaatcggga gcgcgcaaat
     2821 ttctagaggc gaccaaagat cagcaagtgg attttgcgca ggtgatcggt caatttggcg
     2881 tggggtttta ttccgtcttt atggtggcag actgggtgcg ggtcacctct cgttcatata
     2941 agccagacgc agaagcagta agctggtacg ccactggcga ggataactac cagatcagca
     3001 ccgcagagat gagcgaacgc ggcacaaaaa ttgaaattaa actaaaggat gatgctgcgg
     3061 aattcgcgga cgatttccgc ttgcgaaaca ttatcagcaa acattctgat tacatcggct
     3121 tccccattta tattggcgat ggcaatgccc cggtcaacaa gcagacatcg ctttggcgca
     3181 cctccaggca ggaagtcaaa gaggaacagt ataaggaatt ctacaagcag accacattgg
     3241 attttgaaga cccaatgctg cacatccaca tgatcaccga tgcaccggtg cagttgtatg
     3301 ccctgttgta tgtgccggga aaagcggagc gcggtatttt cgccttgcga aaagacgatg
     3361 gactgaagtt atattcacgc aatatcctga tcgatgaata caacaaggat ctattacccg
     3421 aatatctgcg ctttatgcag ggcgtcgtgg attcagaaga tctgccgctc aacgtctcac
     3481 gtgagactgt tcaatccagc gggctgatgc ccaaactgaa aaaagtgctg accaatcagg
     3541 tcatcaagga tctggaaacg ctcgccaaaa ataacgccga gaagtatcaa tccttttggc
     3601 aggagtttgg cggctacatt aaacaagggt tggcaggtga aacggccgac ggcgaaaaac
     3661 tgcactctct actgcgcttc aagaccaatc tgaatcccga aacctggtct tccctggagg
     3721 agtatgcctc tcgaatgaaa gagggacaaa atatcattta ttatattgtc ggcgatgatc
     3781 caaagtctgt gctgcgcagc ccgcatctgg attatttcca cacccaggga accgaagtct
     3841 tactgctaac cgagcccatg gattcattta tgttaatggg cttgcgcaag tataaggacc
     3901 ttgaattgca gaatgtggca caggctgaaa ttgacaccac cgaaacgcca aaggatgagg
     3961 cgcaagctga aaaaatcccg gatcccgatt ttaacgtcct tgtggaacgc ttcaaacaag
     4021 tcctcggcga ccgcgtgaca gacgtgcgcg ccagtaatcg cctctcacag tcggtggcgc
     4081 ggctggctga ccccgatgga acactcaacc ctgaactaca gcgcgtctat agatatcttg
     4141 gtaaagaata cgaagctccc aagaaaattt tggaattgaa tcctgcaaac agtatcttga
     4201 aaggcctgtt gactttagag tctggttcag aacttcaaaa cgcgatcatt gagcaaatct
     4261 atgatagtgc gttgctggta gaaggattgc acccagaccc atcgagcatc gccccacgtg
     4321 ttcagcaaat tatcgaagcg gcgttgaagc aaaattcgag gtaacccgtt ggctgagtag
     4381 gcggtgtgcg ccagatgtga ataaaaaaga tgtccacatg caatgtgggc atcttttttg
     4441 atttatgcct cagagatcac ttttacatag tctgtcgttt tatcaccaag caagccggca
     4501 gtccaatgat ataacgcgta taactcaagt gcgaaaggaa gatatccgcc atagcccaac
     4561 aacggcattt caaagatatg tagccagtcc ccccatggga catcataaat ccactttggg
     4621 tatgaaaagt aattccacat ctcccaaaag aaggcggtca tcaaaacacc aagccacagt
     4681 gcaatgacgg gtctccagtc accttctcgc gtccaatacg cgagggagcg atttccgagc
     4741 cagatgttga ttggctctaa tatgaaataa ggcgaaagcc agatgaagcc aaagaaaatt
     4801 ctgggccaga tcaacagcaa ggcgagcatg atccaaccaa gaataaagaa gaagagaatt
     4861 gtccgtatac cggtgccaat taccggtccg cgcttgagac gtctcacaaa gtcaaagctt
     4921 gcgaaaagtt ctgcgcttcc aaataccgca gggatcacag tcgtaaagct cagggttgtc
     4981 cagaacgcat actccagagg ggaaaatatc tctgaaccga tatacgtcca attttgtgtg
     5041 cgaaggttga gcagctcgaa aaaccaccag accggcgcag atacgagaaa caatccaaga
     5101 tagcggcgag ggcttcgagt gaggagagac gtacccgttc gccaccatac cagcccgtcg
     5161 actgcgagac agtatcccag ccagagaggg aagaatcccc aatgcgtacg cgcacccgtg
     5221 agcgcccaat tgagaatcca gaaaatgagg attaaaccga aaccaatcca gccatgtagg
     5281 ggcagccggg attttgcttt ttgtatggtt cgagtggttg tggtcatgct ccaagtgtac
     5341 cttgagacgc cactcaggct ggaaacaaat aaatttacca aaaaccaaaa tattttgtat
     5401 tttaccaaaa atcttgacaa atcaaaaaat atttgctaat atagcggtca ttggagtatc
     5461 aaatgccact aattccacaa acctgcccta gttgctcctc ccctctcgtg gtcacacagc
     5521 tcaattgcac agcgtgcggc acgggtgtag ttggtaagtt tgagctttcg ccattcttcc
     5581 ggctttcgcc tgaaagcctc aaattccttg aagtctttgt ccgcaaccgc ggcaacgtca
     5641 aagagatgga acgcgagaca ggcgaatcct actgggcgat ccgccgccag ttggatgagg
     5701 tcatcaccga aatgggcatg gaagctccac aggaagatga tctttcgact cgccgccagg
     5761 aaatccttgc caggcttagc cgcggcgaga tcaatgtgca ggaagccacg aaatccctct
     5821 cacaactaaa aggagataaa tcatgaatgg acaaaaagaa cgcctgatgg ttctggatat
     5881 gatcgctgaa ggaaaaatca ctgcagaaga ggccgaacaa cttttcaagg ccatggatga
     5941 ggtgggagac aaactcgatt ccgactcacc tgaacttgtc tcaccgcttt cacatttatc
     6001 tcatctgtcg tcacttaatc cgccttcccc tctgcctggt tcatctcgct cgaaggagct
     6061 ggttgccgca ttaaaagaag ccgggattga ccatgttacc ctgagcgatg tgcaggaaat
     6121 gcaaacccac aggctgacgg cagaatacat tcgcgaaatg ctcgcgcttg gcgttgagcc
     6181 ggaagggctc ggtgaatgga ttaacctgcg cgtgcacgat attacgccgc ggtacgtccg
     6241 cgaactgcgc gagatgggca tgaccgattt tgacgtagat gatctcgtag agctcagaat
     6301 ccatggcgtt tctggaaaat acgccgcagc cttacatggg atcggtctca aaggctttgg
     6361 tgcagatcaa cttgtggaac tcagccaaaa cgatgtgtct gtaaagtaca tcgctgaatt
     6421 acgggaggtg ggactcaaag atttggacgt tgatgaactg atcgagctca gccaaaacga
     6481 tgtctctgca aggtacattg ccgacttgcg ccaggcaggt cttaaagatc tcgatgtgga
     6541 tgatttggtc gaactcagcc agcacgatgt ctctgccaag ttaatcgtgg ccttgggcca
     6601 acagggatta aagagtcttg atattgatga gctggttgaa ctgagcaacc atgatgtctc
     6661 tcccgaattc attgctgaga ttcggcaact gggtttcacg gatctcagtg tcgatgagtt
     6721 ggtggaactg agcaatcatg atgtttctcc tgaattcatc gccgaactgc gtgaaatggg
     6781 actcaaggat tttgatattg acgaagtcat cgaattacgc aaccacgatg tttctgcaaa
     6841 ctttattcgc tccctgcgtg atgtaggcta caccggcttt gatatcgatg acctggtgga
     6901 attacgcatc cacaacgtga ctgcaaagtc agtgcgtgaa ctgcacgaaa tgggatttac
     6961 aaatctgccc gtagatgaat tggtcgaatt gaatattcat catgtcacac cccgtttcat
     7021 ccgggagatg cgtcagaaat atggcgaaga tctgccgctc tccaaaatca tcgagatgcg
     7081 catccatggc gtggatgaag acctgctgga ggaactgcgt gcggcaggag taaaaatcag
     7141 aagataagag cccggaattt aataagtagc aagattcagt ctcgatgcgt cagttgtctt
     7201 cgagactttt gtcaatttgc gggaaacact ttcaatgaaa cagcttcccc atccaccgtt
     7261 cctgataaaa gttggctggg ctgtcccttc aatgtgtgat tcagaaatgc cagcgaatat
     7321 tcttcgatca agctcattag ttcatcggcc tgattggggc caggtaacag cgacggctgc
     7381 agcaaaggtc cgtctgtaaa actctcgtgt gacgcgccat gcaccaccac ccaataactt
     7441 tcagatgtag attcaaagct ctggattagc tttgggtgaa gttgagactc cctggtaagg
     7501 aacataaagg gctgctcggg tggaatggcg gtttcttcca ttgaaaatgg accaccattt
     7561 tgtaaaccat cgtaattcaa acaggcttta aatcgcgcgt ccgctttgca ggattcggaa
     7621 gcggtaatcc cccccaatga gtgtccggca accgcgattg agtccaaatc cattcttccg
     7681 gcaaagcgac cggttgaatt catctgatcc agctgctcca gcacaaacag aatatctgca
     7741 gttctcactt tgattcgttc cctggtatat ctctgatggg ccgtcatatc taaagaccac
     7801 tggtctttgt cgtatggcgc gacatctcca tttgataatt caacagcggg cacatcatag
     7861 gggtgattga tgccaatgac gatgtatcca tggctggcga tttcgctggc gaggcttgaa
     7921 tagaattcca tatttgtgcc attccctggc gagagcatga cgacagggaa cggaatttga
     7981 ttccttaagg gttctgcatc aaaaggaatt tccgagcgaa taaactgtag acccagcacc
     8041 tgccaccatt ccacttcgcc gctttgcagt aatgcatccg atacaaggga cagtaatgga
     8101 aagtagcctg ctttgacacc cgtcccagct tcggctggat accagatcat agcagcaact
     8161 tctcgaaagt catttgggtt ttcggtgaga acttctgggc gcgaggcatc catccaatta
     8221 atggtggttc gacccaccgc ataagggcct tccggtgcgg gcacctcaag ctgagagtac
     8281 accgaaaacg ttaaagccaa catgctgaac agccccataa atactgcaat tttccagacc
     8341 gtgagtcgca taaagtctcc tatccactac atttgacagg agatggaaga aaaagtttcc
     8401 ttcgattacc ctacttgaga gggatttttc gatggcttgc aggaaggtct atttgcagaa
     8461 atttaatgcg gtagcgaggt aaatttcaga gcatgtccga acgctcggaa gatccaccca
     8521 ttcttcgacg gcatgcgccc cggcgccgga aggcccgaac agcacagagg gaattcccgc
     8581 agcggctaat acggcagcat cggtccagta ttgcacacca gcgatttgtg ccggttgacc
     8641 tagcgctgta tcacacgttt cctgaagcac tctgacaatc tcactctctt cggaagtttc
     8701 caaaggtgaa cgatcaatcc ctcgtctaac gaccgcattg aaggttgagt cagactgctg
     8761 aatctcctca atgattttga taaattcggc ttccacggct tcaggtgttt cacccggaag
     8821 tgtgcgtcgt tccacagaca ggacacaccg ttcaggataa ctcgaaagtt cctgtccgcc
     8881 ggtgatcaag gaggcgtgta gagagccgct tcccagtaat ggatgggtgg gattagagcg
     8941 aagcttttga tcgagcctct caatttcgac taacactttt cccatcctgg caatggcatc
     9001 caggcccagg tccgggcgcg agccgtgagc ggctttgcca atcgtttcca cgtcgagcca
     9061 gacaaaccct cggtgagcaa cgattaactg caattccgtc ggctccgcga tgatcgcagc
     9121 atgcgcgtgg taacggtccg caagatccat tgtgccaagg ctggcatatt cctcatcaat
     9181 cacagccgtg aaaatgacgt cgccgctgag cgcatgcttc ttcgcttccg caatggcgac
     9241 catgcatgcg gctactccac atttcatatc ataggcacca cgtccataca agcggccatc
     9301 tttgattatg ggtttatgag cgtcagtcat gccggttatc cccacggtgt ccatgtgacc
     9361 gttgaggagc agggtttttc cgccacctgt gccgcgtgcg atcccgacca cgtttgggcg
     9421 gccagagacc gactcgacca gttccacttc cacatcggcg ctcgttagcc aatcggcaat
     9481 atggtgcgcg atctcccctt ctccgggcga accaggcaca aggtccgggt tgatcgaatt
     9541 gatggaaacc agctcactta acagttgttc aagtttgtcc ataatgaatc tcccgctagg
     9601 aactgtatcg catcatttga tttttgtctt tctgcttttt catcagtgcc gacatgagct
     9661 tgagtccggc gtcgatcgcc acccttccca tcggatcctt cgagagttga tcgtagcgac
     9721 cttcaccaac ggttgagcca tcgaacgcca taatgaaaaa acggacaaag cctttttcgg
     9781 gcacgggcgg agtatcccga agcccaaccc cgatccgctt tgtaatttca aaaccaacac
     9841 tcaacaggtt aaacaccagt ttgtcgatct caggatcacg cgcatcccag atcacgccgg
     9901 tcccggcatt gttccagtag cgagcactgt tatcggaata tccagcgacg acatcgagcc
     9961 ctttgaccgt gtgattttcc atgacaacgc cttgtgtata tccgcgcagc gtatcgatcg
    10021 gcagttcacc caacgcaacc agtgtatgcc aggcctgcag acggatgcgc gtttcgagcc
    10081 cctgtggcgt tgcgacaatt tcttttaatt gcgtgagcgc tttatcttta ttgttctcct
    10141 tcaatgcctg atttgcggca acgaagttgc tccaggggaa cacggacttc gcatcaacct
    10201 gtattcgcgc taaaagcgga tccagcgatt catttgtata aagcgttttc cttaagtcca
    10261 acaggacagg cgaaattcca ccctgcgagt tcggctgttc gtcttgatcc tttttcttga
    10321 aaaatgaaaa cattgaagtc tctcctccat ttatttgttt ctatttattc atataccaat
    10381 gcatcacgga ttgtgagtcg cgccgcactt tgcgccggga taatactggc aatcacagaa
    10441 attatagtca tcaagccgag ccacatcaac atggcaggga ctgaaaaaat ataatccaat
    10501 ggcctggcaa gcagggaaat tcccagggaa tcccccagcc agatcgcaag tggaatcgaa
    10561 catggaatgg acactgccca gctgatcaag ccgattacca atccttcgac aataaccacg
    10621 cggcgcacga ctccattttg tgcaccaagt gatcgtaaaa ttccgatttc acgcgtgcgc
    10681 tcgataacgt tcaagctcat ggtgatggca agcccgagcc cgccaacgag ggcaatcagc
    10741 accaccatgg caagcaaaag cacgatcaaa atatccattt gtgcagcgtt ggtttctttt
    10801 agttgtgtag tagtttgcga gcccaggacg tcgaaattta tcgcatccaa ttgtgtcagc
    10861 acatcagact gcagcgcaga ttgaaaggca gggtcagatc gcgtcgtttg aatcacaagc
    10921 cgattcgcga atccatccaa cccagtttcg tcggcaagct catcgtaaaa catgtatgcc
    10981 accgggacaa agccgcgccc acctgacccg agaattatcc agttttcttc gcgtccggca
    11041 atgttcaatg tgatgacaga accaactttc aaatcgggag agagatccag gaaatccgca
    11101 ttgacgtaaa ttccacgtgt tgtcgtactc gacaaccagc ttccatgaat cacctctggc
    11161 tgtgccatcc gcgtttcttc gggaacgcca tacaaaataa tactgccact taagtgatca
    11221 tcgttgaata caaggcgtcc actatctact gaccaggtct ccaccgcccg gacgccatcc
    11281 agttcaaggg tttcactttc gattttacgc atggaataag gctgcacaaa atcgatcccg
    11341 acgtcgtaat tcaggtcgcc ctgcatttcc tttagtgcct cgcgcatcga ctggcgaatt
    11401 ccgatgatgg cgatgaacat cgcgcctgcc agtaacaatg ccacaaaggt caaagccagc
    11461 cggcctttgc ggcggaatgt gttacgaact gacagattca ccagttgcgg cagttcgcca
    11521 aatagacgaa cagatccgaa acgcgttcca gtttccggct tgtaattgct gatcgcttca
    11581 cgaatggtaa tgagcgagcc ggaaacaatt ggaaataacg aagccaataa gggtgccaac
    11641 aatgcactga atgcctgcaa gaccaatgta attgtcggta agctgaaatg ttctacatta
    11701 aagttcatgc cggtcgcgac gccatcggca aggaagtagg cacccacaag tcccatgggg
    11761 attgctaata gcaaggcaag tatgccaaac aaaagaactt cctgaaaata caaagccatg
    11821 gtctggtgaa tccgccctcc cagagatttc aaaattccaa tttgcggaat ctgttgagac
    11881 ataatggcgg acatcacact tgtgaccagg aaggcactca ggatcaacgt caatacgcca
    11941 agtgcattga ggataaacaa cacggaggtc atattcccgc ccaatgcata ttgattcggg
    12001 atcggcaccg atgcgttaaa gaccggatag cccgcatctt ccaaatcgtc gatcagtttt
    12061 gtcgtttgaa tctcgatctc atttcgtgtg actgcgtttg caaagacgac taacaattga
    12121 ttgtagtgat tgtcgaaacc caactcatcg gccgtatcca gtgagatata accaagccct
    12181 gaaaggctga tctcggacgg caaatgcgac aggtcattga catagccgct cacagccaat
    12241 gtatattcat cgccgttgag gagttgaatc tcgatcttgt cccccacttc cataatgttt
    12301 ttgagcgatc tctcaatcag aataccattg acagccggag gcaaactcgc ctcgccttcc
    12361 agcgtcagtt tgttgatgag gatggaattg aagtcgggaa gagtctgaag ctcgaagccg
    12421 agccaggtat cagatccaga gaggacgcgt gcctgatcaa gccgccgggc ttgggccgaa
    12481 aggataaaat ccagttcctg aacatgcttg agcaggtcat catcaaaagc agaaagagat
    12541 aggatcgtgt gcgcaggttg agtcgcagca tatccgtcac gcaggttttc ttccagcacc
    12601 acacggccgg tgatcatcag accaaaggcg gcgacgccca ctgtgactgc gaggataaca
    12661 agtaaactgc gcgatttgtt gaccagcagg tcacgcagga tctttgtgcg gcgtacactc
    12721 atttctcaac ccttcgatgc accttcgccc ctcaagccga cgcctgttgc ttgcgcgtgt
    12781 cttcaagctg ttgtttcctc gtcccggctg cgccataaat atgtgtcttg aaaatctctg
    12841 atgaatcttc caactggtca aactcccgga cgcccagttc gatgagcttt gccgaatggc
    12901 cctggctcac acggacggtt gcattacgtc gtttatttcc catcaacgcc atttcaccga
    12961 agtaactgcc acgcgacaat tgattgacca gcacttcgct accgcgttca tctctgacga
    13021 gaatatccac ctgaccatcg agtaacaaat agaatgtatc gcccacgtca ccctgtttga
    13081 taacttcctg accctccaga taaattttcg gctccgtgtt ttttagaatc gaactgagta
    13141 cattttcatc gagggaggag agcgtttgaa gatggtggta agtgtttcgt ctttccacca
    13201 gccccatcag ctcttcgcgc agcgtacgcg actcatcgat caacgtattg aatgcgtttt
    13261 catccaatgc ggcgacggag gcaggcccat cctgagatgc ctttacagtt gcccggcgca
    13321 cgccgccacc caacaatgcc atttcaccga aatattgccc cggcttcagt tggttgacga
    13381 atagctcgct gcccccgggc atgttgaggt aaacatccgc tttgccgttg agcaaaatat
    13441 aaaacttgtc acccttctca ccctggcgga caatggcgga attcggctga aagatttgcg
    13501 gattgatcgt gcgcgaaatt tccacgagtt gatcctgcgt caacgaggag agcgcacgca
    13561 ccagatattc gttgaccact tccccatctg agatcaaaat ggtgcggttg acgcggcggg
    13621 caagatcact atcatgcgtg accattagaa tagtcgtgcc accagcgacc agattctcaa
    13681 acagggaaaa aatcttgtcg gcggttttgg agtcaagatt acccgtcggt tcgtctgcga
    13741 tgagtagagg cgggtcgttt gccagagcac gcgcaatggc tacgcgctgc tgctgacctc
    13801 ctgaaatcgc ggagggcagt ttatgcgcct gcgcggtcat ctccactttt tcaagcaggt
    13861 gtagagcgcg ttccttgcgc tctcgtttgg aatacaggtt attcagctcc atcgggagaa
    13921 tgatgttctc caaaatggta agtgtaggaa aaagttgaaa gaattgaaag acaataccca
    13981 ggttccgccc gcgccaggcc gccatttgcc cctcattgaa tgaatggact ggcgcgccgt
    14041 tgatgaggat ctctcctgac gtgggacgat ctatgcctgt gatcatattg atgaaggtgg
    14101 attttccact cccggacttc ccgatgatgg cgacaaattc ccctctctgc acttccacgt
    14161 tgatgccgtt gaccgccgtg aaatcgcctg ctggtgtttg gtagaccttg cgaaggtttt
    14221 tgatctcgat caggggttgg ggtgcaggat tcgatgccat ctccccaagt ttactcgaag
    14281 tgacattttc agagacttct tcatccggcc gcgaaagatt atatggatca accaaaaatg
    14341 caaccggctt aagacagatt cccttaccgg tattgttata cttttgcaat tgagaatcct
    14401 aacattcgtg ctatagtaat cgcacaacat ccgataaagg caaacccggt gaaaaccggt
    14461 gacgcaaagc tatgggtcta acgttccgca cagcggaacc aggactgcca ggccgccgaa
    14521 gaagtcaatc caaactgtac ttgctcgggc ctggctttga taagccaggc tctttttatc
    14581 gggtggcaca ccaacttagt gcaaacccga tcggactcgg ggatgcaaag gagcaaagcc
    14641 atgttacgca atatgaaagt tctgttcgtt ctgttggtcg cgatggtgtt ggcagtgagt
    14701 gcctacgctt ttgcagccgc caatacagtg cccaccacta aggctggaac tggtgcaggc
    14761 gccatcagtg gatacacggt gagcaatgtg gtctataacc tgaacacgtc agaccccaca
    14821 cagctcgatt ccgtggatct cacgctggat gccgcggcta ctaacgtcca gatcaaactg
    14881 gtcgctgccg gctccacctg gtatgactgc accaacgggt caggcaacaa ttggagctgt
    14941 gacacgacct ccccagcaca atcggtcgct tcgatggacg agctggaagt cgttgccaat
    15001 agcaactaat tgaccaagcc tctctaacag gacgggcctg cgcaaggtgg ttccacatgg
    15061 gaaataccag tgcaggcccg atccgtaaaa tggtcctttt atcgcactta tgaaaaagca
    15121 cagcaatcag gctcttcccg gtttcctgat ctcacttttt gtggcggtaa tgttttggct
    15181 tgtgctcgcc ccgacgcaac ttggtgggca ggtcacctat gtgatcgtgg atggcaacag
    15241 catggaacca aagttccatt tcggggattt ggtgctggta cggaaacaaa gcacgtatca
    15301 aattggggat gcggtgacgt atcaaaatgc agaattgggt cgactcgtct ttcatcgtat
    15361 cgtggacatt cagacagatc attttattct tcagggggat cacaacacct ggcttgatgg
    15421 ttatcaaccg aaccagagcg aaattattgg aaagttatgg gttcacattc cagtgctggg
    15481 aaaggcaatt caatgggcgc gggagccgct ccatctggcg atcggctgcg gtttatttgg
    15541 aggcattatg gcggcaggta tcattctcaa tccaaccgga cagaacaaac gaaaaattac
    15601 gaaatccgga ggctatggtg ggttactcga tgttggaatg tatctcactg gattcttcgc
    15661 gctggcactt ttagggctga cgatctttgc gttcacgcgt ccactctcac gcccggtaga
    15721 gaatattcaa tatcagcagg atggaaattt tacttattca gccgcaggca cgcccgatgt
    15781 ctacgacaca gcaaccatcc gacccggaga accgattttc ccgaagttga cctgtttcct
    15841 taacgtggga atggtttaca acttcaccgg cggcggagtg cagcagcttg cgggaaacca
    15901 tcaattctat gcccgtgtat ttgatgaaaa aagtggctgg cagcgtacct tacctcttca
    15961 aacacaaacg gcttttgagg ggaattccta ctccagccgc gcagatctgg acttgtgtca
    16021 gatcgaagcg atggtgactt cagtcgaaga gcaaacaggg ttgcatccta acatttatac
    16081 aatgcaagtg atttcagaga cgacgatcag tggcacactc gataatagcc ctatccgcga
    16141 tacactggat tcgcgcttaa ccttcaaatt cgacgaggta catttctacc ttgaagagtc
    16201 ctcaacgcag gaagacccgc tccatacaac aaaaagcggg atgatcatta cgtctgatca
    16261 gcgacccaac accttgtcaa tcgccggtcg ggaaatcagt atccaaaacc tgcggatgct
    16321 ttccctacta ggtctggcaa tttccgtttt aagcctgttg ccgctaagtt ggtttttttt
    16381 caacaagaaa ctgaatggac aggcggatat gatccgcttg aaatatcgcc atctattgat
    16441 ggatgtccat agaatcaact ttcagcagga cttgcctgtt gttgacgtgg cttccataga
    16501 tgatctggtg aaacttgctg agaggcagag ctcgatgatc atgcacatgg atgtgaatta
    16561 tcttcactat tacaccgtgc aaagcaatgg agtcacctac cgctatgtaa tcagctccag
    16621 gccgggtcaa tctcggcgta gtcaaacgtt gccaagatta gatgcaccgg caaatcctct
    16681 caggcagtac atggaagctg ccccgctgga caatacaaat tacggctacc gggtggacct
    16741 aaatcaaaat gaatatgcgg tacccgatga gtaccagact atatttctaa acaagataaa
    16801 gttatgaaaa cgtcattcct gatgagattt ttttggctca gcatattcag cgtcctgctg
    16861 ttcagcgttg tcactgctgt tgctgcaaca aatacgattc ccaacaccag actcggctac
    16921 aaaaaccttg ctctatttca caacaacctc aagccttctg cctgcgcatc gatttcagta
    16981 actaccctag ttactggggc aggggttatc accggaacca tgggcaatga cttaattctt
    17041 gccagcccgg gtgtggatat catcgacggc tcaggcggaa atgattgtat cgtcggcggc
    17101 agtagcgacg acatccttac aggcggcctc ggtaacgata tttgtcttgg cggtcctggc
    17161 acagatagtt tcatctcgtg tgaaggacaa gaccagtgaa ttttgaaatt tggtatcaag
    17221 cctgctgatg cgatagttta aatttccatc tgagaataca atttatcact gatagcaatt
    17281 tatagaaata ggtttgttca ttcctaaaac ccttctgcca cacgctttgc gtcgccccac
    17341 atgatccaaa gtgctccgca aatctcgccg agagcaatat cgttttctac ggactcaata
    17401 cttattgtta tcaggttctc tgtacccatc gcagcagatt ttaactatcc aagcgaacct
    17461 tccacctgga atggattgac acccaccacg atatgttctt tcacaacaat ggcctgctac
    17521 acggtgagaa tccaacctgg gactaaaatt cttgcacctt ctcatgcgac cggaggatcg
    17581 atattttgag tgcccaccgt c
//
LOCUS       DASTJP010000186         4537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1509494, whole genome shotgun sequence.
ACCESSION   DASTJP010000186 DASTJP010000000
VERSION     DASTJP010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509494"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2448)
                     /locus_tag="VFQ23_05665"
     CDS             complement(<1..2448)
                     /locus_tag="VFQ23_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9906102.1"
                     /translation="MFILPFTSPDVRLETAGGKGANLARLTRAGFQVPRGFIISTDAY
                     RAFVEANRWLSMIESSVDIVSAEDASGLEKISGQIRAAFSVGKIPDEIESVIREAYGN
                     LPHPNPSPSGRGMIEGPGEVIPVAVRSSATAEDLPDLSFAGQQETYLNIFGIDDVLKA
                     VINCWSSLWTARAIGYRMRNHIPQNEVALAVVLQEMIQSDASGVLFTANPLTGLLSES
                     VIDATFGLGEALVSGQVEPDHYVVDIFNRRIVEKKLGAKKISTRGVSGGGVETIDEDA
                     SERQALSDDEILQLADLGEQIQKEYGSPQDIEWAFADGKLYLLQARAITSLYPVPEIS
                     FDPLKVWFSFGAVQGLLQPITPLGQDSIRLVLAGAAGLFGFRLDHESIDFIRVAGERL
                     WVQLDGIVRNPIGARAYKTILPLVEPSSANILTQLAKDPRLKAGSGVVRFSTIGRILR
                     FLLPLIPRFAHTMHNPEKARDEFQGHLARLSGMAHLPREGDMYERLAKCVVLLRDDST
                     IRAFEFVMPRFLPIFAPSVASLGLLTKLAEQNNESDHGVAPLVLEVTRGLPRNVTTEM
                     DLALWDTAKAIRADKDSLELFTTSDAPTLARRYLEGTLTSAAQESITRFMDLYGMRGV
                     GEIDLGQPRWRESPEPIMQNLQSYLKITDELAPDVLFERGAQRAEEAIDTLAKTASQQ
                     SGGWLKEKLVRGAARRVRALMGAREAPKFYAIRVMGVVRTELLAIGEELAKAGVIENR
                     DDLVFLHIRELDALAHREQGDWKALIAERHSRFRREANRKQIPRVLVSDGRAFYEGLG
                     AETDTGDMITGSPVSPGV"
     gene            complement(2479..3600)
                     /locus_tag="VFQ23_05670"
     CDS             complement(2479..3600)
                     /locus_tag="VFQ23_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9906103.1"
                     /translation="MTLKKKTVLGAILFIFIIAGTQLALLYRKPLGPALQLPASTEPS
                     PTLIAFPGSTGEADSTPTEIQSPTVMAATSTATPQPACGGPAVMNILAVGSDARGDHY
                     LYGLADIIRLVRVDFVKKQVGILEVPRDLWVDIPEISDHYGITQGKLNQAYLYGNKGL
                     GYYDGADEGPGLLARTLNLNFGARPDHYLAVNMITFRNIVDAVGGIDVNLPEEISVRS
                     RSNPKGFAIPEGQHHIDGETALWVARIREYSTFSRADNQNIVMCALYKKLMSPASVRS
                     IPQLVTDFQQHVQTDLSPQQISQLACLANEMNGTGVYFAGFPMDMFKNAKVFDPQLKA
                     TTSILEADYDLMRDYIDRFQKGTWPVFDPTLVTEAGICD"
     gene            4156..>4537
                     /locus_tag="VFQ23_05675"
     CDS             4156..>4537
                     /locus_tag="VFQ23_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906104.1"
                     /translation="MKQKISILIGGLLAALMIIGAVGATNVFAQSSTVIAPEHGGGGP
                     GGGRGMGLVGLQAAAEVLNMTTDELITALRSGKMLEEIATEAGVEFQDVRDAINASHQ
                     EELRTRIEQGVADGTISQEKADWLL"
BASE COUNT         1126 a         1202 c         1143 g         1066 t
ORIGIN      
        1 cactcccggt gacacagggc taccggtgat catgtcgcca gtatctgtct ctgcaccgag
       61 tccttcataa aacgcgcgtc cgtcactgac caacacgcga ggaatctgtt tgcgatttgc
      121 ttcacgcctg aatctgctgt gacgctctgc gatcagcgct ttccaatcac cttgctcgcg
      181 atgtgccagc gcatccagtt cgcgaatatg aagaaagacc aggtcatcgc gattttcaat
      241 gactcctgct ttggcaagct cctccccgat ggcaagcaat tccgttcgca caactcccat
      301 cacacggatg gcataaaact tgggcgcttc gcgagctccc atcaaggccc gcacccgtcg
      361 agccgcccct cgcaccaatt tctctttgag ccagcctccg ctttgttgac tcgcggtttt
      421 tgccaatgta tcaatcgctt cttcggcacg ttgcgcgccg cgctcgaaca atacatctgg
      481 cgccagttca tcggtgatct ttagataact ttgcagattc tgcatgatgg gctcagggga
      541 ttcacgccag cgcggttgtc ccaggtcgat ctcgccgaca ccgcgcatgc catacagatc
      601 catgaaacgc gtgatggact cctgcgcggc ggaggtcaga gtcccttcga gataacggcg
      661 agcaagcgtc ggcgcgtctg aggtggtaaa cagttccaac gaatccttgt ctgcgcgaat
      721 agctttggct gtatcccaga gagccagatc catttctgtt gtcacattgc gcggcagccc
      781 gcgcgttact tccagcacca atggagcaac accgtgatcg ctttcattgt tttgttccgc
      841 aagcttggtc aacaaaccga gagacgccac acttggagca aagatcggta agaagcgcgg
      901 catcacaaat tcaaaggcgc ggatcgtgct gtcatcgcgt aacaaaacaa cgcactttgc
      961 gaggcgttca tacatatcgc cttcacgcgg cagatgggcc ataccgctga gacgtgcgag
     1021 atgcccctga aactcatcgc gcgccttttc gggattgtgc atggtatgag caaagcgagg
     1081 aattaatggc agaagaaatc ttaaaattct cccaatcgtg ctgaacctta ccacgccact
     1141 gcccgctttg agacgtggat ctttggccag ctgtgtgaga atattcgcgg agcttggttc
     1201 gaccagaggc aatatcgttt tataagccct cgccccaatc gggttgcgta caatgccatc
     1261 cagttgcacc cataaacgtt cacctgccac tcttataaaa tcgatacttt catggtctag
     1321 cctgaatccg aacaacccgg ccgcaccggc aagcaccaat cgaatactat cctgccccaa
     1381 cggtgtgatg ggttggagca aaccctgcac tgcgccaaag gagaaccaga ctttcaatgg
     1441 gtcaaacgaa atttcaggaa cgggatagag tgaagtaatg gcacgcgcct ggagcaaata
     1501 cagttttcca tccgcgaagg cccactcgat atcctgcgga gaaccatact ctttctggat
     1561 ctgttcaccg aggtcggcaa gctgaaggat ttcatcatct gacagagctt gtcgctcaga
     1621 cgcatcttca tcgatcgttt ccacgccgcc gcccgatacg ccacgagtgg agattttctt
     1681 tgcccccagt tttttctcta cgattcttct attgaatata tctactacat agtgatccgg
     1741 ctccacctga ccggaaacca gcgcttcgcc taaaccgaac gtcgcatcga taactgattc
     1801 gctgagcagc cctgtcaatg gattggccgt gaagagtaca cccgaggcat cactttgaat
     1861 catctcctgc aggacgactg ccagtgcaac ttcattctgt ggaatatgat tcctcatgcg
     1921 ataaccgatc gcacgcgccg tccacagact cgaccagcag ttgatgacgg cctttaacac
     1981 atcatcaatg ccgaaaatgt tgagataggt ctcctgttga cccgcgaagg aaaggtcggg
     2041 taaatcttcg gcagtggcag aggaacgcac tgcaacggga atgacctcac ccggtccttc
     2101 gatcatccct ctccctgagg gagaggggtt ggggtgaggg agatttccat acgcctcgcg
     2161 tattaccgat tcgatttcgt cgggaatttt ccccaccgaa aacgccgcac ggatttgccc
     2221 cgaaattttc tccagaccac tcgcatcttc tgcagaaaca atatccacag atgattcgat
     2281 cattgagagc cagcgattgg cttcgacaaa ggcacgatag gcgtcagttg aaataataaa
     2341 cccgcgcggc acttgaaagc ctgcgcgggt cagtcgagcg agatttgcac ccttcccacc
     2401 tgctgtttcc agcctgacgt ccggtgaagt aaagggcaga ataaacataa agctattatg
     2461 acacaaaagt cgcatccttc aatcacatat tccagcctct gtcacaagcg tggggtcaaa
     2521 aacgggccac gtccccttct gaaagcgatc aatataatct cgcatgagat cataatccgc
     2581 ctcgagaatg gatgtagtag ctttcaattg gggatcaaac actttggcat ttttaaacat
     2641 gtccatcgga aacccagcga aatatacccc tgtcccattc atctcattcg caaggcaggc
     2701 aagctggctg atctgctgtg ggctcaagtc agtttggacg tgttgctgaa aatccgtcac
     2761 gagttgcggg atggaccgca cactcgccgg actcatgagt tttttgtaca acgcacacat
     2821 tacgatattc tgattgtctg cgcggctgaa ggtgctgtat tcccggatgc gcgccaccca
     2881 gagcgcggtc tccccatcaa tgtggtgttg accttcagga atcgcaaaac ccttcgggtt
     2941 ggatcgcgaa cgtacactga tttcctctgg caggttcaca tcaataccgc cgacggcatc
     3001 gacaatattt ctaaaagtta tcatgttgac cgccaggtaa tgatcgggcc gcgcgccgaa
     3061 attgagattc aacgtgcgtg ctaataaccc cggaccttca tccgcgccat cgtaatagcc
     3121 gagtcccttg ttgccatata aatatgcctg gttcaatttc ccctgtgtga tgccgtaatg
     3181 gtctgagatc tccgggatat ccacccacag gtcacgcggc acttcaagga ttccaacctg
     3241 cttcttcaca aagtccacgc gcacgaggcg gatgatatct gccagcccat agagatagtg
     3301 gtcgccgcgc gcgtcagaac caactgcaag gatattcatt acggcaggcc cgccacaggc
     3361 cggttgaggc gttgccgtcg aagtggccgc cataacagtg ggtgattgaa tttcagtggg
     3421 tgtgctatcc gcctcaccgg tcgaacccgg aaaggcaatg agggtgggac ttggctcggt
     3481 ggaagccggc aactgcaggg ctggtcccaa tggtttgcgg tagagtagtg caagctgtgt
     3541 tcccgcgatg ataaaaataa acaaaatagc tccgagaacg gttttctttt tgagtgtcat
     3601 ggcgatgtcc tccgcgatta aaacggaaat gactcaaata aagttctcgc tggttcgcaa
     3661 tcagtttaag gagccaccct gcaagtaaac gaaaacgccc agcctgtctt tcgggctggg
     3721 cgattattag ctactctttt acgagtagtt aggacggata aatttctttc tgaaaatgga
     3781 cgtcattgcg agggcttaag ttatgtggag ggtgtaaagt ctgcgatgtt gatgtggatg
     3841 cgtcgttgtt cgtgtctgag aaatgatatc tatagtgtag ttaagggtct gtgttgtggg
     3901 agtaatgtgc cctcgcaatg acatgtttat tatgacatca agtcattatt ttgaatctta
     3961 cgaacagctt acaactgaaa aaaacaatca aaaatgcaaa ggttatttag cggggcagtc
     4021 agaaacagga cgttaagaaa aaacgatcca cataatttcc ccacaacaat ttcacaggga
     4081 tataacactc tcccgctaag atatagacag gttggcaggt accccagcca taacaatata
     4141 tatcggagat aaaagatgaa acagaaaatt tcaattttga tcggcgggct attggcagcc
     4201 ctgatgatta tcggtgcagt cggtgcgaca aacgtctttg cgcaaagctc tacggtgatc
     4261 gcaccagaac atggcggagg cggccctggt ggtgggcgtg gcatgggatt ggtcggtttg
     4321 caggctgccg cggaagtgct caatatgacc acggatgaac tgatcacggc actacgaagc
     4381 ggcaagatgc ttgaggaaat cgcgaccgaa gctggcgtcg agtttcaaga tgtccgggac
     4441 gcgatcaatg cctcgcatca ggaggaattg cggacgcgta tcgagcaggg cgtagcagac
     4501 ggaaccatct ctcaagagaa ggctgactgg ctgcttg
//
LOCUS       DASTJP010000187         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1058854, whole genome shotgun sequence.
ACCESSION   DASTJP010000187 DASTJP010000000
VERSION     DASTJP010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058854"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..1869)
                     /locus_tag="VFQ23_05680"
     CDS             complement(271..1869)
                     /locus_tag="VFQ23_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9906105.1"
                     /translation="MNSNHYDAIIIGGGHNGLVAAAYLARAGKKVVVLERRHIVGGAA
                     VTEEIFPGYKFTEFSYVVSLLRPEIIRDLELPKHGLKILPLPSTFTPMENGDYLAAWD
                     DHDLTRRELYRHSPKDAEAYNEYARVMARAAKAIKPIINLIPPDPSSLHPRDLFNLMK
                     VGQYAKSLSEKELYTIAKLVTQSSADLLEEWFETDALKGTKAASGIIGTFLGPRSPGT
                     AYVLLHHYMGEIDGAFRAWGFAKNGSGGVSGSIFNAAKALGVETRVNCSVKQVKVRNG
                     RAAGVILENGDELDSKVVMSAADPKRTFLQFVEGKYFPDDFVTSIQNFRTRGSSGKVN
                     IALSELPNFSALPFEGKGADSVLHRGAVSISPSIDYIERAYDDAKYGQISKRPYLDMI
                     FPSMIDPDMAPPGQHVMSCFVQYAPYDLEGGWNDAKRDELGEAVISTIEEYAPNIRKC
                     IVGMQVITPKDIERIAGITGGNIFHGELLLHQIFFLRPTPQWADFRTPLKGYYFGASG
                     AHPGGGVMGAAGMLAAKEILKDGF"
     gene            complement(2020..>2960)
                     /locus_tag="VFQ23_05685"
     CDS             complement(2020..>2960)
                     /locus_tag="VFQ23_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HET9906106.1"
                     /translation="VYYTGWCDDEGKMIDDGTISKLDETTFRMTSADPSLRWLSMNSV
                     GLDVNVTEVTDQVAALSFQGPNSRKILNKVADKPVDELKYFRLMQNNVDGIPVTISRT
                     GYTGDLGFELWMDAKDALTIWDALMDAGQDYGITPVGILAMDMARVEAGLFMIEVDYT
                     STNHAWIEPQKSSPYELGLDWTVALDKQGYFVGRRALEREKRDGSLWKMMGLEVEWEG
                     MEQLFKSVGLPPQIPGMAMRGSLPIMVGNVQVGYSSTSTWSPLLKKYIALAHLQRPYY
                     EVGTNVRMEITVEHHRQHAPAKVVKLPFYEPEWKKK"
BASE COUNT          682 a          828 c          715 g          735 t
ORIGIN      
        1 ataggaagtt ctttaccatt ctctttggtc attgtaaagc ggattgacat ctttgctaat
       61 gaaagcaact gatcactaag atgttcgaat ccagtctcat caagttttgt ttttaattct
      121 tctttgttca tagtgttttc aagttattta gaaaaactca aagcgtagac caaactacat
      181 tcaacacggc ttcgccagtc aaatcttggc tgagaaagaa gcatcaaagg cttcatccac
      241 tcactacttt ctatctctat tccccactct ctagaaccca tccttcaaaa tctccttcgc
      301 tgctagcatt ccggcggcgc ccatgacgcc tccgccaggg tgcgcgccgc ttgcaccgaa
      361 gtaatagcct ttgagcgggg ttcggaagtc tgcccactgc ggcgtgggac gcagaaagaa
      421 gatctggtgc agtaacagtt cgccgtggaa aatattgccg cccgtgatgc ctgcgatgcg
      481 ttcgatgtcc ttcggcgtaa tgacctgcat gcccacgata cacttgcgaa tgttcggcgc
      541 atattcctcg atggtcgaga taaccgcttc gccgagttca tctctctttg catcattcca
      601 tccgccttca aggtcatacg gcgcgtattg cacgaagcag gacatcacat gctgcccggg
      661 tggcgccata tcggggtcga tcatggatgg gaagatcatg tcgagatagg ggcgcttcga
      721 aatctgtcca tacttggcat cgtcataagc gcgctcgatg taatcgatgc tcgggctgat
      781 ggacactgcg ccgcgatgca gaaccgagtc cgcgcctttg ccttcaaatg gcaacgcgct
      841 gaagttaggc agttcactca acgcgatatt gaccttgcca gacgagccac gcgtgcggaa
      901 gttctggatt gacgtgacaa aatcatcggg gaaatatttg ccctccacaa attgcaggaa
      961 cgtccgcttc gggtctgccg cagacatgac gactttggaa tcgagttcat cgccattttc
     1021 aaggatgacg cctgcagcgc gtccattcct gaccttgacc tgcttcactg aacaattgac
     1081 tcgggtctcg accccgagcg ctttcgctgc gttgaaaatc gagccactga ctccgccaga
     1141 gccgttcttg gcaaaacccc aggcacggaa cgcgccgtca atttcaccca tgtagtgatg
     1201 aagtaagaca tacgcagtcc caggcgagcg cggaccgagg aacgtgccga tgattccgct
     1261 ggctgctttg gtacctttca acgcatcggt ctcgaaccac tcttcaagca aatccgctga
     1321 cgattgcgtg acaagtttgg cgatcgtgta cagttctttt tcggatagac tctttgcata
     1381 ctgaccgact ttcatcaaat tgaacaaatc gcgcgggtga agtgacgacg ggtcaggggg
     1441 aatcaaattg atgatgggtt tgatcgcctt cgcggcgcgt gccatgacgc gggcatactc
     1501 attgtaggct tccgcatcct tcggcgaatg gcgatacaac tcgcggcgag tcaggtcgtg
     1561 gtcatcccag gcggcgagat agtcaccgtt ttccatggga gtgaaggtgc tgggtaaggg
     1621 caggatcttg agtccatgtt tgggcaattc caaatcacgg atgatctccg ggcgtaacag
     1681 gctcaccacg tacgaaaact cagtgaactt gtagccaggg aagatctcct ccgtcaccgc
     1741 ggcaccgccg acgatgtgtc tgcgctcgag taccacaacc ttcttgccag ctcgagccag
     1801 ataagcggca gcgaccagtc cgttgtgccc accaccgatg atgattgcgt cgtaatgatt
     1861 actattcatg aacgattcct ttttaccgcc aagacgcgaa gttcgccgag ttttgaaccg
     1921 ccaagcccgc aaagagcgcg aagaaaacct aataaatctt tgcgttctta gcgttcttcg
     1981 cggttaatta ttttcctcat tccaagactc tgcagccaat tactttttct tccattcagg
     2041 ctcatagaag ggcaatttca ctacctttgc aggcgcgtgc tgacggtgat gctctactgt
     2101 gatttccatt ctcacattcg tgcctacttc atagtaaggc ctctgcaaat gcgcgagcgc
     2161 gatatatttc ttcaacagcg gcgaccacgt cgaagtagat gagtatccca cctgtacgtt
     2221 gccaaccatg atcggcaaac tgcctctcat cgccatacca gggatctgtg gcggcaagcc
     2281 cacgcttttg aataactgct ccatgccttc ccattccact tcgagcccca tcatcttcca
     2341 taaagaaccg tcgcgtttct cgcgttccag agcgcggcgt ccgacgaaat aaccctgctt
     2401 atcaagcgcg accgtccagt cgagtcctag ctcatacggc gacgatttct gcggctcaat
     2461 ccacgcatgg ttcgtggagg tgtaatctac ctcgatcatg aacagacctg cctccacgcg
     2521 cgccatatcc atggcgagga tgccgaccgg cgtgatgcca taatcctgcc cggcatccat
     2581 caacgcgtcc cagatcgtga gggcatcttt tgcatccatc caaagctcaa aacccagatc
     2641 gcctgtatac cctgtgcgcg aaatcgtgac gggaatacca tccacgttat tttgcatgag
     2701 acggaaatac tttagctcat caacaggctt gtcggcaact ttattcaaaa tcttcctgga
     2761 gtttggtccc tgaaaactaa gcgccgcgac ttgatccgta acttctgtga catttacatc
     2821 aagcccaacg gagttcatgg atagccatcg taacgatggg tctgcggagg tcatgcgaaa
     2881 tgtcgtctcg tcgagtttgc tgatcgtgcc atcgtcgatc atcttgcctt catcgtcgca
     2941 ccagcccgtg taatagacct
//
LOCUS       DASTJP010000188        13213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_98456, whole genome shotgun sequence.
ACCESSION   DASTJP010000188 DASTJP010000000
VERSION     DASTJP010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98456"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(513..1031)
                     /locus_tag="VFQ23_05690"
     CDS             complement(513..1031)
                     /locus_tag="VFQ23_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906107.1"
                     /translation="MKENQPFQQGSRGCAISLLIIVLAIIALAFSTSYRYPQHISCQG
                     MLGGGVPLLFICDDWGGSSPTSSWGKIDLVDVANGGIQPIGFFIDFVFYVVVSLIVWS
                     AISSFLQKATNHGDLWWATTILIGFLVGFSFAFFSVWTSDQYIKNPPLGTATPVIPSA
                     TAAEPTLTITTP"
     gene            complement(1077..1697)
                     /gene="folE"
                     /locus_tag="VFQ23_05695"
     CDS             complement(1077..1697)
                     /gene="folE"
                     /locus_tag="VFQ23_05695"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET9906108.1"
                     /translation="MGFDNNGAGEDVAINLDVEAAKKLIFDLLKAVGEDPNREGLKNT
                     PDRVARMYAELLSGYSQEPDRIINNALFHVNYDEMVLVRDIEFYSLCEHHMLPFLGRA
                     HVAYIPDGKVIGLSKIPRIVDMYARRLQIQERMTRQIADFIQGTLEPHGVAVVVEAMH
                     LCSMMRGVKKHDARMTTSAMHGSFRANVATRQEFLDNISRPRVSII"
     gene            complement(1761..2330)
                     /gene="raiA"
                     /locus_tag="VFQ23_05700"
     CDS             complement(1761..2330)
                     /gene="raiA"
                     /locus_tag="VFQ23_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET9906109.1"
                     /translation="MANKVETLARNMRLTDNTREYVEKKAAKLPRHLPGIDDIRVELS
                     HAKTARNANDRQVAQITVRGKGFLLRTEERSDDLHGAFDAALEKILRQIDRYKGKHYH
                     GRGDGRSAADVVEEEEMLVDETGELLPLIAKRKKFIILPMNEDEAVEQMRLLGHDNFF
                     IFFNAEQNSIQVLYRRRNGSYGLIEPVVG"
     gene            complement(2373..3110)
                     /locus_tag="VFQ23_05705"
     CDS             complement(2373..3110)
                     /locus_tag="VFQ23_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET9906110.1"
                     /translation="MSNVPWSYRIYRLTWSGLDLLFPPVCGGCGKVGSRWCADCQSRV
                     PYILQPFCEKCGIPIKTNGTCEKCLSNPPAYRKMRSWAVFDSPVQNALHTMKYRRNIG
                     LGEAFATQMADFVRSLGWRAELLLPVPLGKNRLKERGYNQVALVARPLAYEVELDYNP
                     DALWKARETRSQVGLTVSQRRENVEGAYQADIRAVKDKAVILMDDVATTGSTIIACTA
                     ALLSAGAKEVYAITIARALSQHGLTRV"
     gene            complement(3131..4057)
                     /locus_tag="VFQ23_05710"
     CDS             complement(3131..4057)
                     /locus_tag="VFQ23_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9906111.1"
                     /translation="MGLIIFGAVLLIIALAVILVSRRSAAGEDDDPLQARLAEFIQRG
                     DVTSLEEIELSQPFSERVIIPIVRRLGEFSARFTPQKAIQDTARKLELAGNPWPIDAA
                     TFLAIRFILAVVLAGFLIAVVLISPPSNPSDNFMYIGGAAFAGFFLPHLMLTSKITRR
                     QSEIRKAMPDALDLLTICVEAGLGFDAAMSKVSEKWENQLSLAFARTIREVQLGKVRR
                     EALKDMSDRLGIAEMTSFVAAIIQSEQLGVSMAKVLRIQSDQMRVKRRQRAEEEAHKA
                     PIKMIIPMALLIFPSIMIIILTPAALQIMNSL"
     gene            complement(4059..5030)
                     /locus_tag="VFQ23_05715"
     CDS             complement(4059..5030)
                     /locus_tag="VFQ23_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9906112.1"
                     /translation="MSPMVLIIGGVIILVLLIIGVIVSVASERSLVEERLGRYLDEDR
                     PQAQEGESGRSVVTEWLNRRVASSSRGDRIARELARADLKFKVAEYYALVFMSIVVTG
                     FVAWLLQPNIASFAIGAIIGLFIPRFYVKRQQAVRLLKFDNQLSDMLNLMVNGLRAGY
                     STMQAMEAVSRELPTPISDEFRRVVQEMQIGIPTEKALDNLLRRIPSEDLDFVVTAMN
                     VQREVGGNLAEILDIISFTIRERVRIKGEIRVMTAQVRTSGAVLSLIPVFLSLALWFV
                     SPDYIGTFFDESANLPQPLCGIIAVVTIVGMIALGYFVMMKIADIEV"
     gene            complement(5062..6477)
                     /locus_tag="VFQ23_05720"
     CDS             complement(5062..6477)
                     /locus_tag="VFQ23_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET9906113.1"
                     /translation="MSLLKRIEQGQSGGSSGQQPPDNNKGGEGSRLSSLQARRVNAPA
                     TSPQAGSYFDLKTRVQNKLLSELDPSMDITRTDDVRRTIQDLFEQILAEENIVLSRPE
                     RARLFEQIAAEILGFGPLQPLLEDDTITEIMVNGAKNIYIERKGKVHRVPVTFENNDH
                     VMRIIDRIVAPLGRRIDESSPYVDARLPDGSRVNAVIPPISLVGPVLTIRKFSRNPIT
                     VEQLVQFGSVTPEGIQFLKACVEARLNVVISGGTGSGKTTLLNILSSFIPSDERILTI
                     ENAAELQLRQEHVVTLESRPPNIEGRGEITIRNLVVNALRMRPERIVVGEIRDEAALD
                     MLQAMNTGHDGSMTTLHSNGPRDTLSRLETMTLMAGMDLPSRAIREQIASAVDLICHQ
                     ERMRDGTRKITNITEVSGMEGEVITMTDIFVFEQTGVENGQIVGRLRPTGLRPKFMEK
                     IEAAGINLPPSIFGIGERRRY"
     gene            complement(6517..7755)
                     /locus_tag="VFQ23_05725"
     CDS             complement(6517..7755)
                     /locus_tag="VFQ23_05725"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9906114.1"
                     /translation="MPGEKIRVMIVDDVSETRENVRKLLQFESDVDVVGVARTGKEAI
                     QLSQEFNPDVVLMDINMPDMDGIAATEAIRAKQPAVQVVILSVQNDQNYMRRAMLAGA
                     RDFLTKPPMGDELISAIRRAGSMAQSERSKNAQVYVAPVAGNSGMLQGYSGPLGKIVT
                     VYSPKGGAGCTTLAVNLALTLHNEDTRVVLVDGNLQFGDVAVFVNEQGKNTIIDLAPR
                     AEELDPEIVEEVMVKHAASGIHILAAPSRPEYAEKVSSGQFSKVLEYLRQLYAYVVVD
                     TSSLLTDVTLATIDVSDLIVLVTTQDIPAIKNCRLFLDLLQTLGVERDRILFVMNRFD
                     KRINITPDRVADNLKQPVASVIPLDEQTTTKAVNRGVPFVLDSRNQPAARGIFSLAES
                     IRAHMTAQESADESVRSIRR"
     gene            complement(7785..8855)
                     /locus_tag="VFQ23_05730"
     CDS             complement(7785..8855)
                     /locus_tag="VFQ23_05730"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HET9906115.1"
                     /translation="MRRGRIFIFLILIVVVGLALLALVFTQFGQTPVSPTQAAFTDVF
                     YAAQNIPQGTTITREMLGTFSIPPENVAEVMFTTGEESAIVGQTARFTLDQGTMITTS
                     MVGTGPVEISGPAWAVQIPSGMVAAAIPTNRLSLAGYGINDGAHVNVNACMLFVDVDP
                     AYQSVLPNLTSLITGTGFLENQLPTLSAQAVVTLEDGTHPTRQGRIELDPTLQQPFYI
                     VPGEPQRPRLVCQMILQDVVVMKLGNFALQAPSTDPNATPSPAQAQQQASPDIVTLMV
                     NPQDSISLNYFVYSGAMLSMSLRNPNDTSRFSAESATLTSMLTQYNISLPSKLPYASQ
                     PRIDLLQPPVLPNDIVTVQPQQ"
     gene            complement(9014..9163)
                     /locus_tag="VFQ23_05735"
     CDS             complement(9014..9163)
                     /locus_tag="VFQ23_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET9906116.1"
                     /translation="MLFAPKEKGQGLVEYAIILALVAIVVIAVMRLLGPKIGNTFSTI
                     SSSLP"
     gene            complement(9316..10335)
                     /locus_tag="VFQ23_05740"
     CDS             complement(9316..10335)
                     /locus_tag="VFQ23_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9906117.1"
                     /translation="MAMDDISALGGLNLQTELEETQRSLREITLMIEQSQGELSKLSQ
                     RNAAITTHLQQVQNQLDKMPPQEIRSAYDSALDAQQRLFVMRGQLEKLQSEKTHLERF
                     KSTLEQARGSLTSSSTSTSGSKSPLASVEMLVNAQETERQRLSRQMHDGPAQALSNFI
                     LQTEIAMRLLDVDAGQARDELNNLKTSAMGTFQKVRNFIFELRPMMLDDLGLVPTVRR
                     YADAFKEQTGLDISVMITGNERRLEPYLEVMIFRAIQELLGNASRHSQASLVKIMLDL
                     GEERVRVSVDDNGKGFDPEIVQQGNSLGLKLIRERAEMLGGNFEVDSTLGTGARILFA
                     VPARS"
     gene            complement(10342..11043)
                     /locus_tag="VFQ23_05745"
     CDS             complement(10342..11043)
                     /locus_tag="VFQ23_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906118.1"
                     /translation="MNEITLLIADDHPLFRQGVVDTLSLEKDFRIIAQAADGDEAIEM
                     IRSLKPRVAVLDVNMPGLNGQQVTHQVTSEHLPTRVVLLTGYDDIEQAIHAVLAGAVG
                     YCAKDIEPETLARTVRAVADGKYAVGGHIFNRRELDHWIDEQMDGARRSYSEPGSPFH
                     PLSDREMEVLSCVVRGMSNKEIASLLGISHQTVKNHVTSILRKFGVEDRTQAVVYALK
                     RGWVKLQSDQVKHQE"
     gene            complement(11235..12707)
                     /locus_tag="VFQ23_05750"
     CDS             complement(11235..12707)
                     /locus_tag="VFQ23_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906119.1"
                     /translation="MARGSTASNLILDHRLIIALVALTAVSFYLYVSASIFRIGFPLD
                     DTWIHLTYARNLAQHGEWAFRLGESSAGSTAPLWTLLLSIGFLLRLAPYIWTFFLGWV
                     VLTLVGILAENIARKNIETYRPRVPWVGLFFVLAWHLTWSAVSGMETLLHGLFALAVI
                     GALLSQSRRYLALGLLIGLSVWVRPDGLTLLGPLLLTAVLNENSWSSRGQAVLKILVG
                     FGSLFFLYVLFNLLLSGNPLPNTFYAKQAEYEVFWHAKPFSERVIEYLLPIIASPFLV
                     LIPGAILWLAKSVREKNWGALSGAIWVIGYIVIYFASLPAYQHGRYIIPVLPVIYLWG
                     MLGLMGFATSEKASPRIVFLWSSLTGVLCLLFGFIAARQNAHDVYLIESEMVVTANWV
                     NMNVPPDALLAVHDIGALGYFVQNPILDLAGLITPEVVPFIRDEAQIAEYLDASSADY
                     LIVFTSFYPNLTSSRTPVFVAGLEIDPVGIEDDMKVYKWK"
     gene            complement(12670..>13213)
                     /locus_tag="VFQ23_05755"
     CDS             complement(12670..>13213)
                     /locus_tag="VFQ23_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906120.1"
                     /translation="FAGSRGSFFHAGAAFQPYWWVAAPVGLDAVISWARNRGQFTDKN
                     APIVFQGILVLLAALMTFYLVNFRVIANGWAKDDVIYPAVEKMFQDNGIGQQDVVIVR
                     NPPGYFIGSGRSAISLPFGDESTIMAVAEKFDARYLVLEEDGTFESIQDLYDHPQDQP
                     EFIYLGEVDGARLYRIELNP"
BASE COUNT         3678 a         3358 c         2984 g         3193 t
ORIGIN      
        1 tgccttgaaa aacagcttca gtcatagcgg tactccttca atttccaaca tcaatttccg
       61 ataacatcat ccaataattc cgaatcctaa ccaatatgca aagataaagt ataagaacca
      121 taaattaacc gccagcaacg cattaagcgg gattgacatc aaaaaaagtt ttagaggtaa
      181 ttcctcgtga cgaaagaatt gaattatgaa taatattatc caaaccagaa tagagtagaa
      241 cagaacattt tgaatcatcg gtttgccaag tgggtgatct tcaggtccca ggatccccca
      301 gcctcctatt ggagagcctc ctcctggggc atcaacaaaa gctggaaacg gccaaccggc
      361 tttttggtct cccctctgat gcatcacaga acctaaggca atttttcggt tgaacgtatt
      421 gaaatgagat aacagtgaaa cagattgcca ggaatgcgtt tacgaccagc agaaaataag
      481 gggaagtttt catgcgatca ctgctaccga gcctacggcg tcgttattgt aagtgtaggt
      541 tcagctgcag tcgccgaagg tattacaggg gttgctgtcc caagcggagg attcttaata
      601 tactgatcac tggtccaaac tgaaaaaaac gcgaatgaaa aaccgactaa aaagcctatg
      661 agaatggtgg ttgcccacca taggtcccca tgatttgttg ccttctgaag aaaacttgaa
      721 atagctgacc aaacgatcaa actaaccaca acgtaaaata caaagtcaat gaaaaagcct
      781 ataggttgaa ttcctccatt ggctacatcg accaaatcga tttttcccca actgctagta
      841 ggtgaacttc ctccccaatc atcacaaata aaaagtaagg ggacgcctcc gccaagcatt
      901 ccctgacaag atatatgctg tggatatcgg taactcgtcg agaaagccaa agcaatgata
      961 gctaatacga taattagtag gctgatggca cagccgcgag atccctgttg gaagggctga
     1021 ttttctttca tcgtaatcga cctccaattt cagtactcgg tttgatcggt agccagctag
     1081 ataatactta cccgtggcct cgagatatta tcaagaaatt cctgtctggt ggccacattt
     1141 gcgcgaaacg acccatgcat ggcagatgta gtcatccgtg cgtcatgctt ctttacgcca
     1201 cgcatcatcg aacaaagatg catcgcctca acgacaacgg ctacgccatg tggttcgagt
     1261 gtcccttgaa taaagtcggc gatctgacgc gtcatacgct cctgaatctg aagccggcga
     1321 gcgtacatgt ccacgatacg gggaatcttc gataatccga tgacttttcc gtcagggata
     1381 taagcaacat gcgcccgccc gaggaacggg agcatatggt gctcacacag gctgtaaaat
     1441 tcgatatccc tcacaagcac catctcatca taattgacgt gaaacaatgc attattgatg
     1501 atccggtctg gttcttgaga atagccactg agcaattctg catacattct ggcaacccga
     1561 tcgggcgtgt tcttcaatcc ttctcggttt gggtcctcac ctacggcctt gagcaaatca
     1621 aaaatcagtt tcttcgcggc ttcaacatca agatttatag ccacatcttc gccagcacca
     1681 ttattatcaa aacccataaa ttctcctcga caaaccagcc cgacgaacgc cgggctggta
     1741 atagttagtt ttacaggacc ttagccaacc acaggttcga tcaagccata ggaaccatta
     1801 cggcggcgat acagtacctg aatggaattc tgctctgcat tgaagaaaat aaagaaattg
     1861 tcatgaccca acaagcgcat ctgctcgacc gcttcatctt cattcatggg cagaatgata
     1921 aacttctttc gtttggcgat taaaggaagc aactcaccag tttcatcaac gagcatttcc
     1981 tcttcctcaa ccacatcggc ggcagagcgc ccatcaccac gtccgtgata gtgtttgcct
     2041 ttatagcggt caatctgacg taagattttt tccagtgcgg cgtcaaacgc accgtgcaaa
     2101 tcatcactac gctcttcagt tcgaagcagg aagcctttgc ctcgcacggt aatttgcgcc
     2161 acctgacgat cattcgcgtt tcgcgcagtc ttggcgtgtg aaagctcaac gcggatatca
     2221 tcaattcccg gaaggtgacg tgggagtttg gcagccttct tttcaacata ctcgcgcgta
     2281 ttatcggtca gtctcatatt acgagctagt gtttcaactt tgtttgccat agtacctcct
     2341 gaaagattat caatagcgca ctgacgccat tgtcaaacac gagtcaaacc atgctgtgaa
     2401 agtgcacgtg caatggtaat tgcgtaaacc tccttcgcgc ctgctgacaa caaggccgct
     2461 gtgcaagcta tgattgttga gccggtagta gctacatcgt ccattaagat gacagcttta
     2521 tccttgactg cacggatatc ggcctgatat gctccctcga cgttttccct ccgttgtgaa
     2581 acggtcaagc caacttgtga ccgtgtttcg cgtgctttcc ataaggcatc cgggttgtaa
     2641 tcaagctcaa cctcataagc aagaggacgc gcaaccagtg caacctgatt atatcctctc
     2701 tctttcagac gattttttcc aagtggaaca ggcaggagca attcggcgcg ccatcccagt
     2761 gaccgaacaa aatcagccat ttgtgttgcg aaggcttctc ctaacccaat atttcgacga
     2821 tatttcatcg tatgaagcgc attttgcacc ggtgaatcaa aaaccgccca cgaccgcatc
     2881 tttcgatacg caggcggatt cgataagcat ttttcgcacg tgccgttggt cttgatggga
     2941 attccgcatt tttcgcagaa tggttgcaaa atatagggaa cacggctctg acagtcagca
     3001 caccagcgcg aacctacctt tccacaacct ccacaaactg gcgggaagag taagtccagc
     3061 ccgctccagg tcaatcgata aatgcgatac gaccagggta cgttgctcac aacaggataa
     3121 tatgtaggat ttaaagcgaa ttcatgattt ggagtgcagc gggcgtaaga ataatgatca
     3181 tgatcgaagg aaaaattagc aatgccattg ggataatcat tttgatcggc gccttatgag
     3241 cctcttcctc ggctcgttgg cgacgtttca cacgcatctg atcagattgg atccgcaata
     3301 cctttgccat gctcactccg agctgttcgc tctggatgat cgctgccaca aaacttgtca
     3361 tctcagcaat accaagccgg tcagacatat ccttcaaggc ctcacgtctg accttaccca
     3421 gttgaacctc acgaatcgta cgtgcaaacg ccagcgaaag ctgattctcc catttttcac
     3481 tcacttttga catggctgca tcgaaaccca gtccagcctc cacacaaatc gttagaaggt
     3541 ccagcgcatc gggcattgcc ttccgaatct cagactgccg tcgagtgatt ttgctcgtta
     3601 gcattaggtg cggcaggaaa aagcccgcaa atgcagcgcc accaatatac atgaaattat
     3661 cggatggatt ggaggggggt gaaataagaa caacggcaat cagaaagcca gccaagacta
     3721 ccgccagaat aaagcgaatc gcaagaaacg tcgctgcgtc aatcggccat ggattcccag
     3781 ctagttcgag ctttcgggcc gtatcctgaa ttgctttttg cggggtaaac cgtgcagaga
     3841 attcacccag acgtcgcacg attggaataa tcacccgctc gctaaatggt tgggataatt
     3901 caatttcctc gagcgatgta acatcccctc gctgaataaa ttcagccaga cgagcctgca
     3961 atggatcatc atcttcacca gccgcgcttc ttcggctcac caaaataaca gccagcgcga
     4021 taattagcag aacggcacca aaaataatta agcccatctc atacctcgat atcagcgatc
     4081 ttcatcatca cgaaatatcc taacgcaatc attccaacaa tcgtgacaac tgcaataata
     4141 ccgcagagtg gttggggaag attagcgctc tcatcaaaga aggttccaat gtaatctggg
     4201 ctgacgaacc ataacgcaag gctcagaaaa acgggaatca gtgataaaac agcgccggat
     4261 gttcgaacct gggcagtcat gacgcgaatt tcacctttta ttctaacgcg ctcacgaatg
     4321 gtaaaggaga tgatatcaag gatttcggca aggtttccac caacttcgcg ttggacgttc
     4381 atcgccgtaa ccacaaaatc cagatcttca ctcggaatgc gccgcaacag gttatcaagt
     4441 gccttctctg tgggaatgcc gatctgcatt tcttgtacaa cacggcggaa ttcatcagag
     4501 ataggggttg gcagttcacg gctgacagcc tccatcgcct gcatggtgga atatcctgcg
     4561 cgaagcccat tgaccatgag attcaacata tcagacaatt gattatcaaa cttcagaagc
     4621 cgaactgcct gctgtcgctt tacataaaat ctaggaataa aaagaccgat aatcgcgccg
     4681 attgcaaagg atgcaatatt gggctgaagc aaccaggcaa caaatcctgt taccacgatg
     4741 gacataaaaa ctagcgcata gtattcggca accttgaatt tcagatctgc acgcgccaac
     4801 tcacgagcaa tacgatctcc acgggaggag cttgcgacac gacgattcag ccactctgta
     4861 accacacttc gaccggattc cccttcttga gcttgcggcc gatcttcgtc aagataacgc
     4921 ccgaggcgtt cctcaaccag ggagcgttcg ctggcaacag agacgatcac accaatgatc
     4981 aataagacca ggatgatcac gcctcctatt atcaacacca tcggactcat acgaatttac
     5041 ctgccaattt tcaagtgcta attagtaacg tcgccgttcg ccaataccga aaatggatgg
     5101 gggaagatta attccggcag cttcaatctt ttccataaat ttcgggcgaa gtccagttgg
     5161 acgtagacga cctacgattt gtccattttc gacgccggtc tgttcaaaga cgaagatatc
     5221 ggtcattgta atgacctcac cctccatacc gcttacctcg gtgatattag tgatcttacg
     5281 agtgccgtca cgcatacgct cctgatggca aataagatcg acagccgacg cgatctgttc
     5341 acggatagct ctggaaggca ggtccatccc agccatgaga gtcatcgtct ctaggcggga
     5401 caatgtgtca cgcgggccgt tcgagtgcag cgtggtcatg gaaccgtcgt gacctgtgtt
     5461 catggcctgc aacatgtcca atgccgcttc atcgcggatt tcacctacga caatgcgttc
     5521 cggacgcata cgaagagcat taactacgag gtttcgaatt gtaatttcac cgcgtccttc
     5581 gatattcggt ggacgagatt ccaaggtcac cacatgttcc tgccgcagct gtaattccgc
     5641 ggcgttttca atcgtgagga tacgttcatc ggaggggata aaactagaca ggatgttgag
     5701 aagtgtggtt ttgccagaac ccgtaccacc ggaaataaca acattcaggc gcgcctcaac
     5761 gcaggctttc aggaattgga tgccctctgg agttactgaa ccaaactgga ccaattgctc
     5821 aactgtgatc gggttgcgtg aaaacttacg gatcgtaagc acggggccaa ccagtgaaat
     5881 cggtgggatc actgcgttca cacgagaacc atccggcaaa cgagcatcca cgtatgggct
     5941 ggactcgtca atacgacgac caagcggagc aacaatgcga tcaataatac gcatcacatg
     6001 atcgttgttt tcaaaggtca ccggtacccg atgtacttta cccttacgct cgatataaat
     6061 atttttcgca ccgttcacca tgatttccgt aatggtgtca tcttccaaaa gtggctgtag
     6121 gggtccaaat ccaaggatct ctgcggcaat ttgctcaaag aggcgcgcgc gctcgggtct
     6181 ggaaagaaca atattttctt ccgcgaggat ctgctcaaat agatcttgaa tcgtccgacg
     6241 aacatcgtcc gtcctcgtaa tatccatgga cggatccaat tctgaaagaa gcttattctg
     6301 tactcgggtc ttgagatcaa aatatgagcc ggcctgtggc gatgtggcgg gtgcattgac
     6361 ccgtcgcgcc tgcagcgatg aaagacgcga accttctcct cccttattat tgtcaggtgg
     6421 ttgttggcct gatgaaccac cgctctgccc ctgttcaatt cgttttaaga gagacatctt
     6481 tcgttatctc cttgcacaaa ggtcgctgcc aaattcttat cgccgaatcg agcgaactga
     6541 ctcgtcagct gactcctgag ctgtcatgtg agcacgaata ctttctgcga gagaaaaaat
     6601 accgcgcgcg gcaggttgat ttctactatc caaaacaaaa gggacaccgc gattcactgc
     6661 cttcgtcgtc gtttgttcat ccaaaggaat aaccgatgca acaggttgtt ttagattatc
     6721 ggcaacacgg tctggtgtaa tattaattcg tttgtcgaat cgattcatta caaaaaggat
     6781 gcgatcacgt tcaaccccaa gggtctgtaa gagatctaag aacagacggc aattcttaat
     6841 cgctggaata tcctgggtcg ttaccaaaac aatcaagtcg ctcacatcaa tggtggctag
     6901 agttacgtca gtcaaaaggg aggatgtatc caccacgaca tatgcatata gttgccgcaa
     6961 atactccaaa actttactaa actgtccgct ggagaccttt tccgcgtatt cgggtcggct
     7021 cggtgccgca aggatgtgaa ttccagaggc ggcgtgctta accatgacct cttccacgat
     7081 ttcaggatcc aattcttccg ctcgtggggc gagatcgatg atggtattct tgccctgctc
     7141 gtttacgaat acagcaacat caccaaactg aagatttcca tctacaagga ctacacgggt
     7201 atcttcattg tgcaacgtca gggcaaggtt gactgcaagt gttgtacacc ccgcgccacc
     7261 ttttggacta tataccgtga caattttgcc taatggtcca ctataccctt gaagcatgcc
     7321 ggaatttcct gcgacaggcg caacatatac ctgcgcgttt ttagaacgct ctgactgggc
     7381 cattgaccct gcacgacgga tcgccgaaat taattcatcg cccatcggcg gcttggtcag
     7441 gaaatcgcgg gcgcccgcca gcattgcgcg gcgcatatag ttctgatcgt tttggactga
     7501 aagaattacc acttgcacgg ccggttgttt tgcacgaatg gcttctgtag ccgcaatgcc
     7561 gtccatgtct ggcatgttga tatccatcaa gacaacatcc ggattaaact cttgagagag
     7621 ttgaatcgct tcttttccag tgcgcgcaac accgacaaca tcaacatcag actcaaattg
     7681 tagaagtttc cgaacgtttt cccgcgtttc agaaacatcg tcaacgatca taactcgaat
     7741 tttttcaccc ggcattggac aattacctcg atcttttgct aatattactg ctggggttgc
     7801 acagtaacaa tatcatttgg caaaactggt ggctgcaaca aatcaattct gggttgtgag
     7861 gcatagggca acttggacgg aagagagata ttgtattgcg ttagcatact tgtcagagtt
     7921 gccgactccg ctgaaaaacg agacgtatcg ttagggttac gcaaagacat acttaacatt
     7981 gcgccgctat aaacaaagta attgagggaa atggaatcct gggggttcac cattaatgtc
     8041 acgatatcag gtgaagcctg ttgttgagcc tgagccgggg atggtgtggc gtttggatcc
     8101 gttgaaggag cttgtaaggc aaaattaccc aacttcatga caaccacgtc ctgaaggatc
     8161 atctggcaaa ccaaccgggg ccgttgcggc tcaccgggaa caatgtaaaa cggctgttga
     8221 agggtaggat caagttcaat acgtccctga cgagtcggat gcgtaccatc ttccaatgtc
     8281 acaactgctt gtgctgaaag agttggtaac tgattctcca ggaagcccgt gccagtaatc
     8341 aacgacgtaa gattgggaag gacagactga taagccggat ctacatccac aaacagcata
     8401 caagcattta cgttaacgtg tgcaccatca tttatgccat aaccagccaa agaaagtcga
     8461 ttggttggta ttgcagccgc caccattccg gatggaattt gaactgccca ggcaggtcct
     8521 gaaatttcca ccggtcctgt tccgaccatg gaagtggtaa tcattgtccc ttggtccagt
     8581 gtaaagcgag ctgtttgacc aacgatagcg ctctcctcgc cagtcgtaaa catcacctct
     8641 gcaacattct ccggtggaat ggaaaatgtt cccaacattt ctctcgttat ggttgttccc
     8701 tgaggaatat tttgtgcagc gtaaaaaaca tccgtgaagg cggcctgagt cggtgacaca
     8761 ggtgtctgcc cgaattgagt aaatactaag gcaagcaacg ccaggccaac cacaacgatc
     8821 aatataagga atatgaatat tcgtccgcga cgcatagaga aaatctcctc tcttgactcc
     8881 ttgccggcta acttggcagg tattccaaag gaatattata cagtatacac gaaaaaccaa
     8941 gatgttaatc tggtaaaaaa gtaggactcc gcctgaggca gagtcctact ttttccttgc
     9001 cagaaggcaa ttactaaggc agagagctgg aaatcgtact gaaggtgtta ccgatcttgg
     9061 ggccgagcaa gcgcatcact gcaataacaa caatggcgac gagagcgaga ataatcgcgt
     9121 actcaaccaa accttgacct ttttcctttg gagcaaataa catgacagca attcctttct
     9181 tgaataaacg tttacgccga ggactttcct cagatttttt tattttactc aggccccaat
     9241 ttttttcaag agatgagaag caaaattgct tgcaatcctg caaataattc ctaacattct
     9301 tgaaatgaaa acgaactagg agcgtgcggg aactgcgaat aagattcttg ccccggtacc
     9361 caaagtgcta tccacctcaa aatttccacc cagcatttcg gcgcgctcac gaatcaactt
     9421 caaacccaaa ctattgccct gctggacaat ttcaggatca aagcccttcc cgttatcatc
     9481 gacgctgacg cgaacgcgct cctcgccgag gtccaacatg attttcacca aacttgcctg
     9541 gctatgacga gacgcattgc caagcaattc ctggatcgca cgaaagatca tcacctcaag
     9601 gtagggctcc aaacgacgtt catttccggt aatcattacg ctaatatcca gcccggtttg
     9661 ctctttaaac gcatccgcat agcgacggac cgtaggaacc aagcccagat catccaacat
     9721 catgggacgc aattcaaaga taaagtttcg aactttttga aacgttccca tcgccgacgt
     9781 tttcagatta ttcaactcgt cgcgggcctg accagcatca acatccagga ggcgcatggc
     9841 aatctcagtt tgaaggatga aattggaaag ggcctgggcg gggccatcat gcatctgcct
     9901 cgagagacgt tgacgttccg tctcctgcgc attgaccagc atctcgacac tggccagggg
     9961 acttttcgat ccggacgttg aggttgaact ggaggtcaat gagcctcgcg cctgctcgag
    10021 ggtcgatttg aagcgttcaa ggtgagtttt ttcgctctgc aacttttcga gttgtccgcg
    10081 catgacaaac aagcgctgtt gggcatccaa ggcggagtcg taggcgctgc gaatctcctg
    10141 cggcggcatc ttatccaact ggttttgtac ttgctgaaga tgggtggtaa ttgccgcgtt
    10201 acgttgtgaa agtttggaaa gctcgccctg gctctgctcg atcatcaagg tgatctcacg
    10261 caaagatcgc tgggtttctt caagttcggt ctgcagattc aatccgccaa gcgcagagat
    10321 atcatccata gccataaaca attactcctg gtgtttcact tgatcgctct gcagtttcac
    10381 ccagcctcgt ttcagtgcat acacaacggc ttgtgtacga tcttccaccc cgaattttcg
    10441 aaggatcgag gtgacatgat ttttgacagt ctgatgacta atgccaagca aacttgcgat
    10501 ctctttgttg ctcataccgc gcacaacaca ggacaatact tccatttcac gatcagataa
    10561 ggggtgaaac ggcgaaccgg gctcactgta tgagcgccgc gcgccgtcca tttgttcatc
    10621 gatccagtga tccagctcac ggcgattaaa aatatgaccc cctaccgcat attttccgtc
    10681 agctacggcc cgcacagtac gagccagcgt ctcgggttcg atatcttttg cgcagtatcc
    10741 gacagcgcca gctaatacag catgaatggc ctgctcgata tcatcatagc cagttagcag
    10801 aactacacgc gtcggcaaat gttcagaggt gacctgatgc gtcacttgtt gaccattgag
    10861 ccccggcatg ttgacatcca acacggctac tcttggctta agtgaacgta tcatttcgat
    10921 tgcttcatcg ccatccgcag cttgcgcgat tatccgaaaa tccttttcga gggaaagtgt
    10981 atccacgact ccctggcgaa atagcgggtg atcatctgca atcagcagtg ttatctcatt
    11041 catccgtggc ctgttctttt tggaaaagta attaccgctt gtctcaaaaa taggtaaact
    11101 tttgatggat ttgcaggcta aaaatcatct caaaagttta accatttata aggcactcta
    11161 taattgagta tagcacagga gcgaccagcg ggcaatttat ctattatctc tttacatttc
    11221 agtttcgtaa tatatcattt ccatttataa accttcatat cgtcttcgat ccccaccggg
    11281 tcaatttcca acccggcaac aaaaacaggc gtacggcttg aggtgagatt tggataaaaa
    11341 cttgtaaaaa caatgagata gtctgcagag cttgcatcaa gatattcagc aatttgcgcc
    11401 tcatcacgaa taaatggaac aacctcgggg gtgattagtc ctgccaggtc gagtattgga
    11461 ttctgtacaa aataccctaa cgcaccaata tcatggacgg ccagcaatgc atctggggga
    11521 acgttcatat taacccaatt tgcagtaaca accatctcgc tttcaatcaa ataaacatca
    11581 tgtgcgtttt ggcgggcagc gatgaatcca aaaagcagac agagaacgcc ggttaaagaa
    11641 gaccataaga atacgattcg cggactggct ttttcagacg tcgcgaatcc catcagacca
    11701 agcatccccc acaaataaat aacaggcaac acagggatga tatatctacc atgttggtag
    11761 gcaggcagac tggcaaagta tataacgatg tatccaataa cccagatcgc tccactcaat
    11821 gctccccagt ttttttcacg aacacttttt gcaagccaca agatggctcc tggaataaga
    11881 acaagaaaag gacttgcgat gatgggcaaa aggtattcga taacacgttc gctgaaaggt
    11941 tttgcatgcc aaaacacctc atattcagcc tgcttggcat agaacgtatt tggaagcggg
    12001 ttacctgaaa gcaagaggtt aaacaatacg tataagaaaa ataaggagcc aaagcccacg
    12061 agtattttca acaccgcctg tccgcgagaa gaccaagaat tttcattgag gacggcagtc
    12121 aacaacaatg gacccagcag ggtcaagccg tcgggccgaa cccagacact taatccgatt
    12181 aataagccta acgccagata acgacgtgac tggctaagca gtgcaccaat caccgccaag
    12241 gcaaagagcc cgtgaagcag ggtttccatt cctgaaacag ctgaccaggt aaggtgccag
    12301 gcgagaacga aaaataaacc aacccaggga acacgtggcc tgtatgtttc aatgttcttg
    12361 cgcgcgatgt tttcagcaag aatccctacg agagttagaa ccacccagcc aagaaaaaaa
    12421 gtccagatat aaggagccag acgaagaagg aagccaatgg aaaggagcag agtccataac
    12481 ggcgcggtgg aaccagccga actctcaccc aggcgaaacg cccactcccc atgctgtgcc
    12541 agattccgcg cgtaggttaa atggatccag gtatcatcaa gagggaagcc aatgcgaaag
    12601 atcgacgcgc tcacatacaa gtagaagctg accgcagtca aagcgacgag ggcaatgatc
    12661 agccgatggt caaggattaa gttcgatgcg gtagagccgc gcgccatcca cctctcctaa
    12721 atatatgaat tcaggttgat cctgtgggtg atcatataaa tcctgtatcg actcaaacgt
    12781 gccatcttct tcaaggacaa gataacgagc atcgaacttt tccgcgaccg ccataattgt
    12841 agattcatca ccaaacggca gggatatagc agaacgtccg gaaccaataa agtagccagg
    12901 cgggttgcga acaatcacta catcctgttg gccaatgccg ttgtcttgaa acattttctc
    12961 caccgcagga taaatgacat catcctttgc ccagccattt gcgatcacgc gaaagttgac
    13021 gaggtaaaac gtcatgagtg ccgctaacaa gactaatatt ccctgaaaaa caatcggagc
    13081 attcttatcg gtgaattgac cgcggtttcg cgcccatgaa atgaccgcat ccaacccaac
    13141 gggcgccgcc acccaccaat acggttggaa cgctgcgccg gcgtggaaga aacttccgcg
    13201 cgaaccagca aac
//
LOCUS       DASTJP010000189         8916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1279344, whole genome shotgun sequence.
ACCESSION   DASTJP010000189 DASTJP010000000
VERSION     DASTJP010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279344"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1079)
                     /gene="add"
                     /locus_tag="VFQ23_05760"
     CDS             complement(36..1079)
                     /gene="add"
                     /locus_tag="VFQ23_05760"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET9906121.1"
                     /translation="MAAERVTASLNTYRALPKVELHRHLEGSLRLDTMVDIAQKHGIE
                     IPEDVLRLSTMVQVQEEDKFTFQNFLSKFNTLRLFYRSPDVIHRITREAIEDAAKDNI
                     RYMELRFTPVALSRAERFPLHDVIDWVISSTKEASEKSGVIVRLIASVNRHESADLAE
                     QVAWLAIEHFKDGMVALDLAGNEAEYPSEPFYGIFKEAKQSGLHVTIHAGEWGPAKHV
                     KEAIEQIGAERVGHGVRTLEDKSIVDLARERGTAMEVCITSNYQSGVVQSLDTHPLMD
                     MLEAGLNATINTDDPSICRITLSHEYYTACEELQMSQTILRERIVAAAQAGFLANDEK
                     DKLVEQLKRDLKM"
     gene            complement(1093..5100)
                     /locus_tag="VFQ23_05765"
     CDS             complement(1093..5100)
                     /locus_tag="VFQ23_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906122.1"
                     /translation="MKIFGGDPNKKTIEQYSGIAKEINALESQFESLSDEALRAKTGE
                     FKSRIIELMGNVDGLDEKEQFKAEQDALNEILPEVFAVVREASKRTIGLRHYDVQLIG
                     GVALHRGSIAEMRTGEGKTLVATMPIYLNALLGKGVHLITVNDYLARRDARWMAPIFQ
                     FLGLSIGVLQMAAVTENGKKAFLVDFERESANEDQDHLRMVDRVEAYAADVTYGTNSE
                     FGFDYLRDNMTMRLSDRVQRGHHYAIVDEVDNVLIDEARTPLIISGPASGDLEWYGKM
                     FQVVRQFRPEDYELNEKDRAVSLTEVGLARAEQLLNQPLMDPDRPEDVTPEQARLRGY
                     LEQAMRAQFIFKRNKDYLVQAGKVVIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIEP
                     ENVTYATITLQNYFRMYKKLAGMTGTALTEAEEFSKIYNLEVTPVPPNLEYQAFGNNA
                     PLVEVKAKDEEGYAYTYFAKRESRGAGEPQALFYRRKDYPDIIYKTVEAKLRSIVMEI
                     IREHVRGRPLLVGTTSVESSDQLSNRLKAESVRRLLQIALVRQVWMEANKREEDGRLI
                     PELQQFNEPLEKVTPDSLRKFIQPFGLTNINPEDPANLPIVLDLLRLSDQNVERLKKV
                     LQGGIPHQVLNARKHTEESQIIAGAGGFGAVTIATNMAGRGVDIKLGGELAEEIITAV
                     NRVLGRAGYDNPFDMTQEERRQALKNVDPSNYGLYDAEVKHFLEYFENMELVKQLGGL
                     HVIGSERHEARRIDNQLRGRSARQGDPGSSRFYLSLQDDLMRLQGGSQVSGLMERLRV
                     DDSLPLEVRVVGNIIEQSQHRIEGANFDVRKHLLEYDDVLNKQRAQIYGQRDRIFVKE
                     DLSDDVDEMLELEVRQRVETGLADEEGPWKLIAWLEQVQPPFMSGERLFPSFGMSLLL
                     KEISSSDDFRASAIDIISRAIDAENEHNLSAIDALIDSTEQSLEAQTAAREDTLDAYF
                     EGLRDMDDTERPRPQKIVEEINNLLGMQIRLNNEQLRTLGEDPQEIKEDIHDLVSANL
                     TAVYASRVIAAVQNRFGEALGEKFEVSNWDDAAEKILEAAQGALNRRRERLVGDAGQI
                     ARDIDNLMPRELNDTSKLQLLLTLSQGARTAFDQKTHRQVKQVFSRFSYVFLIARLLE
                     GIEADQVVEDVLSHLDEAEESLQRAWGEREFNRLSANAQRLADFGTAAKMAFGEERLN
                     EPLSSLGESDRDALIESVGKYVLNEVHRQLLLGATSELWVDYLTRIEALRVSIGLEAY
                     AQRDPLVQYKGRASEMFAQLVEDIRGLVIGRIFAYRPRPVEITPVESTDAPAPATTQT
                     APQIDNGKKKKRRRH"
     gene            complement(5333..>8916)
                     /locus_tag="VFQ23_05770"
     CDS             complement(5333..>8916)
                     /locus_tag="VFQ23_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET9906123.1"
                     /translation="VGLAIFELVRYRKLEHLLLVGYVVITFLYHAQMFVKFMRYFLPI
                     YPFLILFAAYFIMWLWRYANLTQEYRTHESDLPSSLWKQWLSRVRISRQLVVSIAAVV
                     AGGTLIYAMAFSSIYGRMNSRLQASRWMYQNLPVGSTLANEHWDDWLPIGGLDGNTSF
                     GEQGRFQSVEMANYEDDTTDKLNRMVENLTQADYIVLSSNRLYDSIPRLPMRYPMTSR
                     YYELLFSGKLGFERVKEFTSYPIFLGIQIPDQAAEESFSVYDHPRVQIFQKTAAFDPA
                     TVRQELSDGIVWENVIHLRPLEASSVAKSLELSATEEALYQRTATWSSAAVRGGSWGG
                     RFPALAWFIALQLIGLIALPLTFIAFETFADRGYIFSKVIGLLLVAWGAWLIASIRLA
                     PFTWWTILAVMAILVLGSALWITNQKWNELLIFIRTKWRLLLLEEVLFWIFFAILFVI
                     RWHNPDLWHPGMGGEKPMDVAYLTAIVHTPYFPSYDPWFSGGYINYYYFGFVLASTLI
                     HLTGIVPTIAYNLAVPTFFALMAMGAFTVALNFAERQQNESQGEWSRPLGIGRTALIA
                     GLGGALFVAVLGNFGQVKLLWDGIRNMSSIPAGENTSVQVTLAQFGDGLGKLIGGEDL
                     SLRTEWWYWNATRVIPPAEGEAGPINEMPFFTFLFGDLHAHMMALPYTLLALGLALNL
                     IRSSWRTAEDANTLRWWRDPVEILTLTLAALSTGALWPMNTWDFPTYSVLIIAALCCR
                     EYARRGRVDLYGLWAVAWRTALIVIVGRLLFLPFHLNYASAYFGAELWKGSRTPLWAY
                     LLIHGFFLFVITSYLIAELLRGPGHNALVRSLRLNIRYWRRRGRMQRLFNHLTHPSST
                     YHLIIDISQLALVLVIVTLLVNQVIGLILLLTLLTALLLFSARPHPLRQFLLCMIGIG
                     LMYTALVEIVVLRGDISRMNTVFKFYLQVWVLWGIVSAAVLPKLATWMKIQLSQKRAP
                     LPELEEGSAWTPQVARETERYQRGPANAWPRRWWWAFSILLAACFLYPVTAAPVRVDD
                     RFENSISRTIDGTAYMKTSTYMDDSRPITLDWDLQAFEWLKENVHGIPTILEANTPLY
                     RWGSRVSIYTGLPTVIGWDWHQKQQRSILPGQTIERRIEDVRTIYDGTNLEETKKLLG
                     LYNVQYIYVGPLERLYYASDGLDKFDQPSDLWDLVYENEQVKIYQVH"
BASE COUNT         2219 a         2354 c         2158 g         2185 t
ORIGIN      
        1 cgcctttgat acccttgggg tcttcgtggt gcgatttaca ttttcaggtc tctttttagc
       61 tgctccacta acttatcctt ttcatcattt gccaggaacc ctgcttgtgc agccgcaacg
      121 atccgttctc tcaaaatcgt ctgcgacatt tgcagttctt cacaggccgt gtaatattcg
      181 tggcttaatg taatgcgaca gatgctgggg tcatcggtgt ttatggtggc attcaagcca
      241 gcttccagca tgtccattaa cggatgcgta tccagcgatt ggaccacacc cgattgataa
      301 ttgctcgtga tacaaacttc catcgcggtt ccacgttcgc gtgcgagatc tacgatactc
      361 ttatcttcca gtgtacgcac accatgacct acacgttctg cgccgatctg ttcgatcgct
      421 tccttcacgt gcttggccgg accccattcg cctgcatgaa tggtcacatg caagccgctt
      481 tgtttggctt ccttgaatat cccgtaaaaa ggttcagatg gatactcagc ctcattgcca
      541 gccaggtcca gggcaaccat accgtcctta aaatgttcaa tcgccagcca ggcaacctgc
      601 tctgcaagat ctgcactttc gtgacggttc accgaggcga tcaatcgtac gatcacaccc
      661 gacttttcag acgcttcctt cgtgctggaa attacccaat caatcacatc atgtaatggg
      721 aaacgctctg cccggctgag agcaaccggc gtaaagcgta actccatata tcgaatgtta
      781 tctttggcag cgtcttcgat ggcttcacgg gtgatccgat gaatcacatc aggagaacga
      841 tagaacaacc gcaaggtatt gaacttggac agaaaattct ggaaggtaaa cttatcttct
      901 tcctggacct gcaccatggt actgagtcgc aagacatcct ctgggatttc tataccatgc
      961 ttctgcgcga tatccaccat ggtatccaaa cgtagcgagc cttccagatg gcgatgtaac
     1021 tccaccttgg ggagggcacg atacgtattg agagaagccg tgactcgttc tgctgccata
     1081 cgtttgatga gtttagtgtc gtcgccgttt cttcttctta ccgttatcta tttgtggtgc
     1141 cgtttgagtg gttgccggag caggtgcatc tgtgctttca accggtgtga tttcaacagg
     1201 tctcggacga taagcgaaaa ttctgccgat gacaagtccg cggatatctt ccaccaattg
     1261 tgcgaacatt tcagatgcac gacctttata ttgtaccagt gggtcgcgtt gtgcgtaagc
     1321 ctccaaccca atcgagactc tcaatgcttc aatgcgagtt agataatcca cccacagttc
     1381 ggaagtagcg ccaagcagca attgtcggtg gacttcattc agcacatatt ttcccacaga
     1441 ctcgatcaac gcgtcgcggt cagactcacc caggctggat aatggttcgt tgagtcgctc
     1501 ctcaccgaaa gccatcttgg ctgccgtccc aaaatctgct aggcgctggg catttgcaga
     1561 gagacggttg aactcgcgct caccccatgc acgctgcaag gactcttcgg cttcatcaag
     1621 atgactcaat acgtcttcga caacctggtc tgcttcgatt ccttcaagca accgcgcgat
     1681 caaaaacaca taactgaagc ggctgaagac ctgctttact tgtctgtgtg tcttttggtc
     1741 aaaggctgtc cgggcgccct gtgaaagggt aagcagcaat tggagtttgc tggtgtcatt
     1801 tagctcacgc ggcatcaggt tatcgatgtc tcgcgcgatc tgaccggcgt ccccgaccaa
     1861 tcgttcacgc ctacggttga gcgcgccttg tgcggcttcg aggatttttt cggcagcgtc
     1921 atcccaattg gaaacctcga atttttcacc gagggcctcc ccaaatcgat tctgcaccgc
     1981 ggcgatgacg cgggaggcgt agactgcggt caggttggca gagaccagat cgtgtatgtc
     2041 ttctttaatt tcctgtgggt cttcacccag tgtgcgcagt tgttcattat tgagcctgat
     2101 ttgcataccc aggagattgt tgatttcctc cacgatcttt tgaggccgcg gtcgctctgt
     2161 gtcatccatg tcgcgcaagc cttcgaagta agcgtccaac gtgtcttccc gggcagcagt
     2221 ctgtgcttcc aggctttgtt ccgtactatc gatcaaagcg tcgattgcac ttaagttatg
     2281 ttcattctct gcatcaatgg cacgcgaaat aatatcgatc gcggaggcac ggaagtcatc
     2341 cgaacttgaa atttccttta gcagcaaaga catcccgaag cttgggaaga ggcgttcgcc
     2401 agacataaac ggtggctgaa cctgttcgag ccaggcaatt aacttccagg gaccttcctc
     2461 atcggcaagc cccgtttcga cgcgctgccg cacttccaac tccagcattt catcgacatc
     2521 gtcgctcaaa tcttctttta caaaaatgcg atctcgttgg ccgtagatct gggcacgctg
     2581 tttattgagc acatcgtcgt attcaagcag atgcttgcgc acgtcgaagt tggcaccctc
     2641 gatacgatgc tgggattgtt cgataatgtt gccaaccacg cgcacttcaa gaggtaacga
     2701 gtcatccacg cgcaggcgtt ccatcaaacc gctgacctgc gagccgccct gcagtcgcat
     2761 tagatcatcc tgaagcgaaa gataaaaacg ggaggagccc gggtcaccct gacgagccga
     2821 acgaccgcgc agctggttgt cgatgcggcg ggcttcgtgt ctttctgaac cgataacatg
     2881 caagccgccc aattgcttta ctaattccat attttcaaag tattcaagga agtgttttac
     2941 ttccgcatcg tataggccat aattggatgg gtcaacattc tttagagcct gacggcgttc
     3001 ttcctgagtc atatcaaagg gattatcata acccgctctg ccgagcacgc gattaaccgc
     3061 agtaatgatt tcttccgcca gttcgccacc aagcttgata tccacaccac gacctgccat
     3121 attggtagcg atggtcacag cgccaaaacc accagcgccg gcgatgatct gggattcctc
     3181 ggtgtgttta cgtgcgttca atacctgatg cggaataccg ccctgtaata ctttctttaa
     3241 acgttccaca ttctgatcgc tgagcctaag gaggtccaga acaattggca gatttgccgg
     3301 gtcttcgggg ttgatgtttg tcaacccaaa cggttggatg aacttccgca gcgaatcagg
     3361 ggtcaccttt tcaagtggct cattgaattg ctgcagttca ggaatcaggc gtccgtcctc
     3421 ttcgcgttta ttggcttcca tccagacctg tcgaaccagc gcgatctgta acaagcggcg
     3481 cacagactct gctttgagtc ggttcgaaag ctggtcggag gattctaccg aggtagtgcc
     3541 aaccagtaat ggacgcccgc gcacatgttc gcggataatt tccatcacga tcgagcgcaa
     3601 tttcgcttcg accgttttgt agataatatc tggataatct ttgcggcgat agaaaagagc
     3661 ctgtggttca cctgcgccac gagactcgcg tttggcaaaa taggtgtagg catacccttc
     3721 ttcatctttt gccttcactt caacaagcgg cgcgttgttg ccaaacgcct ggtattcaag
     3781 gttgggcgga accggcgtta cctcaaggtt ataaatcttg ctgaactctt ctgcctcggt
     3841 gagggctgta ccggtcatcc cagccagctt tttatacatg cggaagtaat tttgcaacgt
     3901 gatggtggcg taggtcacat tttcaggttc gatcttcaca ccttctttgg cttccaccgc
     3961 ctgatgcaaa ccatcgctcc accgtcgacc gggcatgagc cgccccgtaa attcatccac
     4021 gatgacaacc ttgccagcct ggacaagata atccttgttg cgtttgaaga tgaactgcgc
     4081 gcgcatagcc tgttcaagat agccgcgcaa gcgcgcttgt tcaggagtta cgtcttcagg
     4141 gcggtcggga tccatcagag gctgattcaa aagctgctca gcgcgcgcca ggcccacttc
     4201 cgtgagagag acggcacgat ccttttcatt caattcgtaa tcttccggtc tgaactgccg
     4261 cacgacctgg aacattttgc cgtaccattc aagatcgcct gaagcgggtc ctgagatgat
     4321 gagcggtgtt cgcgcttcgt cgatcagcac gttatccact tcatcgacga tcgcgtaatg
     4381 atgcccgcgc tggacccggt cgctcaagcg catggtcata ttgtcgcgca ggtaatcgaa
     4441 accaaattca gagttggttc cataggttac atctgcggca tacgcctcca ctcgatccac
     4501 catccgcaga tgatcctgat cttcatttgc ggactcgcgt tcaaaatcca cgaggaaggc
     4561 tttcttaccg ttttctgtca cagccgccat ttgcaatacg ccaatagata acccaagaaa
     4621 ctggaagatg ggagccatcc agcgcgcgtc gcggcgtgcc aggtaatcgt tgaccgtgat
     4681 gagatgcacc cctttgccaa gcagtgcatt gagataaatt ggcatggtag cgaccagcgt
     4741 tttaccttcc ccggtgcgca tctcggcaat cgagccgcga tgcagcgcca cgccaccgat
     4801 caattgaaca tcgtaatgtc gtaaaccaat tgtgcgttta gaggcttcac ggacgacggc
     4861 aaacacctca ggcaatattt cgttgagagc atcctgctca gccttgaatt gttccttttc
     4921 atccaagcca tccacatttc ccatgagttc gatgatgcgt gatttgaact cgcctgtctt
     4981 ggcgcgcaac gcttcgtcac tgagggactc aaactgagac tcaagagcat tgatctcttt
     5041 tgcaattcca ctatattgtt cgatcgtctt cttattggga tcgcccccga aaattttcac
     5101 aaaattcttt agcataatga ccctttcgga agaaaattat actgcttctt ccacgaatgg
     5161 cacgaataaa tttgttacac gttgagcata acagtgtagc acgcgaatgt tagcgaaaag
     5221 taaaaattgg gtggcggatc tggtccgcca cccaaaatag gtcagaaatt agttgactat
     5281 ggcccgaaaa cgcctacctg tgagctactc ccctaaatca atctggacgt ttttaatgga
     5341 cctgatagat ctttacctgc tcattctcgt agacgagatc ccacaaatcg cttggctgat
     5401 cgaacttatc caggccatca ctggcatagt agagacgctc cagaggtccc acataaatgt
     5461 actgcacatt atataagccg agcaattttt tagtctcttc aaggttggtg ccgtcgtaga
     5521 tggtgcgcac atcttcgatg cgacgttcaa ttgtttgtcc cggcaggatc gagcgctgtt
     5581 gcttctgatg ccaatcccag ccgataacag tgggcaaccc ggtgtagatt gagacgcgcg
     5641 agccccagcg atagagaggt gtattggctt cgagaatcgt gggtatgcca tgcacattct
     5701 ctttcaacca ctcgaatgcc tgaaggtccc agtccagtgt gatgggtcgg ctatcatcca
     5761 tataggtaga tgttttcata taagccgtgc cgtcaatcgt cctggaaata ctattctcaa
     5821 atcggtcatc tactcttaca ggagcggcag taactggata gaggaaacaa gcggccaaca
     5881 gaatactaaa tgcccaccac caacgtcgag gccaggcatt tgctggtccg cgctgatacc
     5941 tttcagtctc tctagccacc tgtggagtcc aggcgctgcc ttcctccagt tcgggtaaag
     6001 gagcacgttt ttggctcagc tgtattttca tccatgtcgc cagttttgga agaacagctg
     6061 ctgagacaat cccccagagc acccacactt gcagatagaa cttgaatacc gtgttcatgc
     6121 ggctgatgtc accccgaaga acgacaattt caaccagtgc tgtgtacatc aaaccgattc
     6181 caatcatgca aagcaaaaat tggcgaagtg gatgtgggcg ggcactgaag agcaatagcg
     6241 ccgtcagtag ggtcaataaa aggataagtc cgatgacttg attaaccaag agagtaacga
     6301 tgaccagcac caaggccaat tgactgatat cgattattaa gtggtaagtg gatgaaggat
     6361 gagtgagatg gttgaatagt cgttgcatgc gcccacgacg gcgccagtat cggatattga
     6421 gtctgagtga gcgaaccaac gcgttatgtc cggggcctct gagcaattca gcaataaggt
     6481 aagaagtgat cacaaaaagg aaaaatccgt gaatcaggag ataggcccac agaggggtac
     6541 gtgaaccttt ccaaagctca gcgccgaaat aagcactcgc ataattgaga tggaatggaa
     6601 ggaaaagcaa tcgcccgacg atcacgatca acgcggtacg ccatgcaacc gcccaaagac
     6661 catataaatc tactcggccg cgacgcgcgt actccctgca acacaaggcc gcgatgatca
     6721 aaacactgta cgttggaaaa tcccaggtgt tcatgggcca tagggcaccg gttgacaacg
     6781 ctgctaacgt tagtgtaaga atctccaccg ggtcgcgcca ccagcgtaga gtattcgcgt
     6841 cctctgctgt gcgccatgaa gagcggatta aatttaatgc caacccaaga gcaagaagcg
     6901 tgtaaggcaa tgccatcatg tgagcatgga ggtcaccaaa caggaaagtg aagaatggca
     6961 tctcgttaat tgggccggct tcaccttccg cgggtggaat cacgcgggtc gcgttccagt
     7021 accaccattc ggtgcggagg gaaagatcct ctccgccgat caatttaccc agcccatcgc
     7081 cgaattgtgc cagtgtgacc tggacggagg tattctctcc agcggggatc gaactcatat
     7141 tccgaatccc gtcccataat agttttacct gaccgaaatt gccaagcact gccacaaata
     7201 aagcaccgcc gagcccggca attaatgcgg tacgtcctat accgagcggg cgactccatt
     7261 ctccctggct ctcattttgc tgcctttcag cgaaattgag agcaacggtg aaggcgccca
     7321 ttgccatcag agcaaaaaat gttgggacag ccagattata ggcaatcgtc ggcacaattc
     7381 cggtcaagtg gatgagtgtc gatgccagaa caaagccgaa ataatagtaa ttgatataac
     7441 caccggaaaa ccacggatca taggagggaa agtaaggcgt atgtacaatc gcggtgaggt
     7501 atgccacatc cattggtttt tctccaccca tacccggatg ccataagtcg gggttatgcc
     7561 aacggatcac aaacaggatg gcaaagaaaa tccagaataa aacttcctcc agtagcaaaa
     7621 gccgccactt cgtgcggatg aagataagca attcattcca tttttggttg gttatccata
     7681 gcgcggaacc aaggacaagg attgccatca ctgccaggat tgtccaccag gtgaatgggg
     7741 cgagacgaat gctggcgatc agccatgctc cccatgcgac aagcaaaagg ccaataacct
     7801 tgctgaaaat ataacctcgg tcagcaaaag tttcgaacgc gatgaacgtg agagggagag
     7861 cgatcagccc aattaactgg agcgcaataa accaggccag cgcaggaaaa cgaccacccc
     7921 aacttcctcc tctgactgcc gcgctggacc atgttgctgt tcgctgatac agggcttctt
     7981 ccgttgctga gagttcaagc gattttgcaa ctgaactcgc ctcaagcggt ctgagatgta
     8041 tgacgttttc ccacacaatc ccatcactga gttcctggcg aaccgttgcg ggatcaaaag
     8101 cggcagtctt ttgaaagatt tgcacgcgcg gatgatcgta gacgctaaat gattcttcgg
     8161 ccgcctgatc tggaatttga attcccagaa agatagggta cgacgtaaac tccttgacgc
     8221 gctcaaaccc cagtttgcca ctaaagagca actcatagta acgtgacgtc atcgggtacc
     8281 gcatgggcag tcgagggatc gagtcataca gacgattact tgagagcacg atgtaatcgg
     8341 cctgggtgag attctccacc attcgattta atttatcagt cgtgtcgtcc tcgtagtttg
     8401 ccatctcaac cgattggaac ctgccctgtt cgccaaacga tgtatttcca tcaagtccac
     8461 cgattggcag ccagtcatcc caatgttcat ttgccagtgt ggaaccaaca ggaaggtttt
     8521 gatacatcca gcgcgacgct tgaagacggc tgttcattcg accataaata gaactgaagg
     8581 ccatcgcgta gatcagagtt ccacccgcca caactgctgc gatggacacg accagttgcc
     8641 ggctgatccg gacgcggcta agccattgct tccataggct tgaaggcaaa tccgattcat
     8701 gagtccgata ttcctgagtg agatttgcat atctccacag ccacatgatg aagtaggcgg
     8761 caaacaaaat caaaaacggg tagatcggca ggaagtagcg catgaatttg acaaacattt
     8821 gagcatgata gagaaatgtg atcaccacat agccaacaag taacagatgc tcaagttttc
     8881 gatacctcac caactcaaaa atcgccaatc ctaccc
//
LOCUS       DASTJP010000190         7152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1395014, whole genome shotgun sequence.
ACCESSION   DASTJP010000190 DASTJP010000000
VERSION     DASTJP010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395014"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFQ23_05775"
     CDS             complement(<1..215)
                     /locus_tag="VFQ23_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906124.1"
                     /translation="MMTDQNKESAISFLKLASSGKVREAYAKFVGEGFRHHNPYFEGS
                     AEALEVGMQENADQNPEKTLDVKQVLA"
     gene            complement(227..703)
                     /locus_tag="VFQ23_05780"
     CDS             complement(227..703)
                     /locus_tag="VFQ23_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HET9906125.1"
                     /translation="MEEDKTRLQSALQPEKPEHPLRRLDPVVGKWDLRGRTLDTAEDN
                     ITGWVNFEWLPGGFFLEARGEIDFNGNKVQSLEILGYDPETDTFPANVYSNTNGTVLK
                     YYWDIKENTVTHWTEGSEYTGSFSDDGKTLSGGWRPQAGNEGNPESTYDAVMTRVD"
     gene            823..1644
                     /locus_tag="VFQ23_05785"
     CDS             823..1644
                     /locus_tag="VFQ23_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9906126.1"
                     /translation="MKQTKEWSNIKHNTHLDVGFLHARYIHYAYPRHSHDYYVISLIE
                     RGRQSFTHKGERYTTPAGGVILINPGVVHTGEPVDEQGFELRSLYPTLAHMKMAAFEL
                     TGRHVALPFFNDIRVDHPWVSESILSLHRSLMEGSTSLETESRFVWFLTQLIKFYGET
                     SLGEQKAGDENQAIRKARHFIEEHFAHSIRLDALARHVALSPYYFLRVFCAQVGMPPH
                     AYQESVRIRHAQRLIREGKTLAETAMEVGFSSQSHMTRSFKKIIGVTPGQYAQDL"
     gene            1670..2266
                     /locus_tag="VFQ23_05790"
     CDS             1670..2266
                     /locus_tag="VFQ23_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HET9906127.1"
                     /translation="MTTSFLSIVSYVLVSTFTPGPSNISSASMAVLHGYKKTLSFQTG
                     LAIGVFILMVVIGWFSKTLLNVFPSLEPIMRYVGAGYILYLAFGILKASYSITEDKTK
                     PLGLANGLMLQILNPKLTVYAFTLFSAFLAPITANVAILLFAALTLAVTSFCATSLWA
                     LFGAAIQSYLLAPRWRFVVNLLLSVSLIYTALTLIRPN"
     gene            complement(2263..3117)
                     /locus_tag="VFQ23_05795"
     CDS             complement(2263..3117)
                     /locus_tag="VFQ23_05795"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET9906128.1"
                     /translation="MILITGAGGKTGKAIVKSLSSTESISALVHREENVSALKSLGAE
                     KVIMGDLRDDDAIRYSMQGIRAVYHICPNMSRDEFAIGKLVINEARKAGVGHFVYHSV
                     LHPQVEKMNHHWQKMHVEEMLFESGLTFTILQPAPYMQNLLAGWKSILEDGVLRVPYS
                     VHSKFSFIDLEDLAEAAKVVLTQTDHKNAVYELVGTLPISHVEVAEIVSRVLDHDVRA
                     EKEEIKDWRLRAADLSEYATDNLVKMFEYYDEWGLVGNSNVLRLILEREPTSLEAFFR
                     RNVRGHDH"
     gene            3254..3643
                     /locus_tag="VFQ23_05800"
     CDS             3254..3643
                     /locus_tag="VFQ23_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9906129.1"
                     /translation="MQTANKFTMVSIVVSDMKKAKEFYAEKLGLTVATDYRRNDNNWW
                     VTLNFPGGGANITLARSSTTHNEPAKPDTISFYFSTSDLAAAHKNLNDNGVTTSEIMD
                     DLYGPGSGVKFFQVKDPDGNQITFAQE"
     gene            3758..4108
                     /locus_tag="VFQ23_05805"
     CDS             3758..4108
                     /locus_tag="VFQ23_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9906130.1"
                     /translation="MKYMLLLYANESVELTTPEEQEAVAPEAWFALMKEMNDAGVLIS
                     NNGLAPTATATTVRVREGKALITDGPFAETHEQLGGFFLLECQNLDDALRWAERIPHA
                     MYGSVEVRPLWSEV"
     gene            4125..5351
                     /locus_tag="VFQ23_05810"
     CDS             4125..5351
                     /locus_tag="VFQ23_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9906131.1"
                     /translation="MTTESQIEKTFREEHGRVLAALIGQLGDFMLAEDALQDALVNAL
                     ERWSVDGVPRNPGAWLTTVAKRRAIDRLRRSRFEEDSEPLPTSLAQEEPEMDDSLPDD
                     RLKLMFTCCHPALALEAQVALTLHTLGGLSTPEVARAFLVAEPTMAQRLARARRKIRD
                     AGIPYRVPPADLLPERLEALLATIYLIFNEGYVATTGEALVRRELCMEAIRLCRIVVN
                     LLPSSRNAEARGLLALMLLHDSRRETRLTPTGELVLLEEQDRALWDQEKIHEGIAVLD
                     SAIVLRDSGPYQIQAAISALHAEAKTYAETDWTQITMLYQALARMTPSIVVEVNRAVA
                     VAMAQGVEKGLKMLLDLESQANHFYPYHAALADLLRRTNQPEAAADAYQRALDLCGNT
                     AERAYLQRRLNEMLNP"
     gene            5429..5794
                     /locus_tag="VFQ23_05815"
     CDS             5429..5794
                     /locus_tag="VFQ23_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9906132.1"
                     /translation="MLNLNSVMIGTKQSAALAAFYEKVLGKPADMVDQENGFWGWQVG
                     STYLSVLEHSEMGGSTKDPGRVMFNFETEQVKEEFDRITAAGATVIREPYDMGGGWIA
                     TLADPDGNYFQLVTPMVSQ"
     gene            5808..6221
                     /locus_tag="VFQ23_05820"
     CDS             5808..6221
                     /locus_tag="VFQ23_05820"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9906133.1"
                     /translation="MPNNYIEKVIDINAPVSKVWRVFTDPFLSRQMGGEYVSEWKVGS
                     SFSWKGLDGQLLTNGSIVAIEPEKLLQHRLLSSDETSAESEISMITYKFEEANGRTTL
                     RAREDFSHPVSDEEFDGAAEGWEAALQSVKEIAEK"
     gene            6281..7111
                     /locus_tag="VFQ23_05825"
     CDS             6281..7111
                     /locus_tag="VFQ23_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9906134.1"
                     /translation="MESLTKRSGFVANGNARIYYETAGAGSPFVMIHAGVADHRQWNN
                     EFASFATNHHVVRYDMRGYGKSEPVEGEFNPMDDLVSVLDVLELHEPIVLMGCSMGGG
                     LAMDFALKYPARVKALIMVGSGPSGLELDVPAPAKFAEAEKAYESGDLDLLAEIETQI
                     WFDGTGRSPEQVDQTMRNLAYEMNRLALAHESKQLGKRLPNAETPAFDRLADLKIPVL
                     IIVGEHDTPYILAAADYMLQNIPSARRVLIEDAAHLPNMDHPEEFENAVKDFLDRLSH
                     "
BASE COUNT         1842 a         1795 c         1725 g         1790 t
ORIGIN      
        1 tccgcaagaa cctgtttcac atccagggtc ttctccgggt tttgatctgc gttctcctgc
       61 atcccaacct caagggcttc tgccgaccct tcaaaatatg gattgtgatg ccgaaatccc
      121 tcccccacaa actttgcgta cgcttcgcgg acttttccac tggaagccag cttgaggaag
      181 ctaattgcag attctttgtt ttggtctgtc atcatgattg ctcctatcaa tcaacgcgag
      241 tcatcaccgc gtcatatgta ctttctgggt tgccctcgtt gccagcctgc ggtctccacc
      301 cacctgagag agtctttcca tcatcgctga aactaccggt gtattccgaa ccctccgtcc
      361 aatgcgtgac ggtattctct ttaatatccc agtaatattt caagacggtc ccattcgtat
      421 tggaataaac gtttgcggga aaagtatctg tctccggatc atagcccagg atttcaaggc
      481 tttggacttt gtttccatta aagtctatct cacctctggc ttcaagaaag aacccgcccg
      541 gcagccactc aaaatttacc caacccgtaa tattgtcttc tgcagtatca agcgtccgac
      601 ctcgcaggtc ccacttccct accactggat ccaaacgcct aagcgggtgt tctggctttt
      661 caggctgtaa tgcggattga agcctggttt tatcttcttc catatgactt tactcctccg
      721 tcgctcttaa cgacgcaact ctctggctgt tctgactgaa tctgccagat tgagcaattt
      781 tctgcaagac acataaagtg gacttatgat aaaaagagaa cgatgaagca aacaaaagaa
      841 tggtcaaaca tcaagcataa tactcatctg gatgtgggat tcttgcatgc cagatatatc
      901 cactatgctt atccacgtca cagccatgat tactatgtaa ttagccttat tgagcggggc
      961 agacaatcct tcacccataa aggtgaaaga tacaccacac ccgctggcgg cgtcatcctc
     1021 atcaacccag gagtggttca caccggagaa cccgtggatg aacaaggctt tgaattgcgt
     1081 tctctttatc ccactctagc acatatgaag atggcggctt ttgaactgac aggccgccac
     1141 gtggcgcttc ccttttttaa cgacatacga gtcgaccatc cgtgggtgtc agagagcatt
     1201 ttatctttgc atagatccct gatggagggc tcaacttcac tggaaactga atcgcgcttc
     1261 gtttggtttc ttacacagct tatcaaattt tatggcgaga cgtcgcttgg tgaacaaaaa
     1321 gccggtgatg agaaccaggc tattcgaaaa gcgcgccact ttatcgaaga gcattttgct
     1381 cacagcattc gcctggatgc gctcgcaaga catgttgcgc tgagtccata ttacttcctg
     1441 cgggtcttct gtgcccaagt aggaatgcct ccacacgctt atcaggaaag cgttcgaata
     1501 cgtcatgccc aaaggctaat ccgagaaggc aagacgctgg cggagacagc aatggaagtg
     1561 ggctttagca gtcaaagcca catgacgcgt agtttcaaga aaatcattgg ggttacacca
     1621 ggtcaatacg cacaggactt atagcttata aaagcaattg ggtattcaaa tgacaaccag
     1681 cttcctttcg atcgtttctt acgtgcttgt atcaaccttc acaccggggc cgagcaatat
     1741 ctccagtgct tcgatggctg tccttcatgg atataagaaa acgctgagct tccagacagg
     1801 gctggcaatc ggtgtcttta tcttaatggt agtgatcgga tggttctcta aaaccctatt
     1861 gaacgtgttc ccttctcttg aaccgatcat gcgctatgtg ggcgcgggct acattcttta
     1921 cttagcgttt gggatattaa aagcgagtta ttccattacg gaagataaga cgaaaccctt
     1981 aggcttggca aatgggttga tgctgcaaat cctaaatcca aaattgacgg tttatgcttt
     2041 tacgttattc tctgcatttc tggcacccat aaccgcgaat gtagcaatcc tgcttttcgc
     2101 tgcactcact ctggctgtaa cgtcgttttg cgccacttcg ctatgggcat tatttggggc
     2161 agccattcag tcctatcttc ttgcgccaag atggagattc gttgtcaacc ttctattatc
     2221 cgtttccctt atatatacag cgctaacgtt gatcagacca aattaatggt catggcccct
     2281 gacatttcta cgaaaaaaag cttccagcga cgttggttcc cgttctaaga tcaacctcaa
     2341 aacattcgaa ttgcccacta gtccccattc atcgtaatac tcaaacatct taaccagatt
     2401 atccgttgca tactcgctca ggtcagcagc acgaagtctc cagtctttga tctcttcctt
     2461 ctcagcccgc acatcatgat ccagcacgcg actcactatt tcggcaactt ccacatgtga
     2521 gattggcaat gtaccgacaa gttcataaac agcattttta tggtccgttt gagtcaatac
     2581 aactttcgcg gcttcggcaa gatcttccag gtcaatgaaa ctgaattttg aatggacaga
     2641 gtaaggaacc cgcagtacgc catcctcaag gatgcttttc cagcctgcca gcaagttttg
     2701 catatatggc gcgggttgca agatagtaaa tgtcaatccc gactcgaaga gcatttcttc
     2761 cacgtgcatt ttctgccagt ggtgattcat cttttccacc tgggggtgca acacggagtg
     2821 atagacaaaa tgccccactc cggctttgcg cgcttcgtta atgactaact tgccaatcgc
     2881 aaattcatcg cgactcatat tcgggcaaat gtgataaacc gcccttatgc cttgcatcga
     2941 ataacgaatt gcatcgtcgt cacgcaggtc acccatgata accttctctg cacctaaaga
     3001 ctttagcgca gaaacgtttt cctcgcgatg aactaacgca ctaatgctct cagtacttga
     3061 gagtgacttt actatcgcct tgcctgtttt tccaccggcg cccgtgataa ggatcatatt
     3121 tggtctatta taaagactac ccagcaattt atgagagaat tatgagaatt acgttcacct
     3181 gtcattttag cggctcgctg tgcgacgtat tggtagaaag gtaaaaacaa tatatttcta
     3241 caaggagtct atcatgcaaa ctgctaacaa attcacaatg gtttcgatcg tagtcagtga
     3301 tatgaaaaaa gcaaaagagt tttatgcgga gaaactcggc ttaacggttg caacggatta
     3361 ccgccgaaat gataataact ggtgggtaac gcttaacttt cccggcggcg gggcaaatat
     3421 tactcttgcg agatcgagca caacgcacaa tgaaccagcg aaacccgaca ccatttcgtt
     3481 ctatttttca acgtccgacc ttgcagccgc gcacaaaaat ctcaatgaca acggtgtcac
     3541 aaccagtgag atcatggatg acttatacgg tccgggctca ggcgtgaagt tttttcaagt
     3601 caaagacccg gatggtaatc agataacttt tgcacaagag taaaatcctg catccattgc
     3661 gaagcctgat cttcagggaa gcggtatgat taatgcatcg caacgatcga aagaaacgca
     3721 ggtagaccat tcatcccaaa taaaggagaa ggatcgaatg aaatacatgt tattgctgta
     3781 tgccaacgag tcggtggagc tgaccactcc cgaggagcag gaagccgttg cacccgaggc
     3841 ctggtttgcg ctgatgaaag aaatgaatga cgccggcgtg ctgatctcaa acaatgggct
     3901 tgccccaaca gcgacggcaa cgacggtgcg cgtccgagaa ggcaaagccc tgattaccga
     3961 tggtcccttt gccgaaacac acgagcaatt gggcggtttc tttctgcttg aatgccaaaa
     4021 ccttgacgat gcacttcgct gggcggagag aattccgcat gccatgtatg gctcagtgga
     4081 agttcgccca ctgtggtcag aggtttaata attttgagcg tgggatgaca accgagtcac
     4141 aaatcgagaa aaccttccga gaagagcatg ggcgcgtgct cgcggctctc atcgggcagc
     4201 ttggcgattt catgctggct gaggatgcgc tgcaggacgc gctggtcaac gcgctggaac
     4261 gctggagtgt ggatggtgtt ccgcgtaacc ctggcgcgtg gctaaccacg gttgcaaaac
     4321 ggcgcgccat cgaccgcctt cgacgttccc ggtttgagga agactcggag ccacttccca
     4381 cttcgctggc gcaagaggaa cccgagatgg acgactcact tcccgacgat cgactcaagc
     4441 tgatgtttac atgctgtcat ccggccctgg cgttggaggc gcaggtagcg ctcacgttac
     4501 acaccctggg cggtttgtct acgccggagg tggcacgcgc ctttctggtt gccgaaccga
     4561 ccatggcgca acgtctggca cgggcgcgca ggaaaatccg cgatgccgga atcccgtatc
     4621 gtgtaccgcc tgctgacctc ctccccgaac gactggaggc cttactcgct acgatctact
     4681 tgatcttcaa cgagggatat gtcgccacaa ccggtgaggc gttggttcgc cgagaactgt
     4741 gtatggaagc aattcgactt tgtcgcatcg tagtaaattt gctcccctct tcgcgaaacg
     4801 cggaagcacg cggactcctg gcgttgatgc tactacacga ctcgcgacga gagacgcggc
     4861 tcactccgac gggtgaacta gtgctgcttg aggaacagga tcgcgccctc tgggatcagg
     4921 aaaaaataca tgaagggatc gccgttctgg attccgcaat tgttttgcgg gattccggtc
     4981 cttatcagat tcaggcagcc attagcgcat tacatgcgga agcaaaaacg tatgcagaaa
     5041 cagactggac tcaaatcacg atgctctatc aggcattagc gaggatgact ccatctatcg
     5101 ttgttgaagt gaatcgagcc gtcgctgtcg caatggcgca aggcgttgaa aagggtttga
     5161 aaatgttatt ggatctcgaa tcgcaggcaa atcactttta tccgtatcac gcagcactcg
     5221 ccgacttatt gcgcagaacg aatcaacccg aggctgccgc cgatgcgtac cagcgcgcgc
     5281 ttgacttatg cggtaacaca gcagaacgcg cctatctcca gcggcggctc aatgagatgt
     5341 tgaacccgta acgattaagt accttgtcaa aacagaatat gcgttctaca atgagtaatc
     5401 cctatcgatg tttagaaagg aaaatcagat gttgaactta aattctgtca tgatcggaac
     5461 gaagcaatcc gctgccttgg cagcattcta tgaaaaggtt ttgggaaagc cagcagacat
     5521 ggtcgatcag gaaaatggtt tttggggttg gcaggtgggc agcacgtatt tatcggtcct
     5581 ggagcattca gaaatgggcg gcagtacaaa agacccgggc cgcgtgatgt tcaattttga
     5641 aacagaacaa gtcaaagaag agtttgatcg aatcacggcg gctggtgcca ccgttatccg
     5701 ggagccctac gatatgggag gcggttggat cgcgactctg gcagacccgg acggaaacta
     5761 ctttcaattg gtaaccccaa tggtcagcca atagaatctc agaaaagatg cccaacaatt
     5821 acatagagaa agtaatcgat ataaacgccc cagtttcgaa agtgtggcgc gtgtttacag
     5881 atccatttct tagcagacaa atgggcggcg aatacgtctc cgaatggaaa gtgggttcat
     5941 ctttttcgtg gaaggggctc gacggtcaac tattgacaaa cggctcaatc gtggccatcg
     6001 agcccgaaaa gcttctccaa cacaggctgc tgagcagcga cgaaacatca gccgagtctg
     6061 aaatatctat gatcacttac aagttcgaag aagcgaatgg acgtactaca cttcgggcac
     6121 gagaggattt ttcacatccg gtctctgacg aagaatttga cggagctgca gaaggttggg
     6181 aggctgcact gcaaagcgtt aaagaaattg cagaaaagta acagcattac gaaaaaagtt
     6241 ttttacttct cattagaagt ttggtcaaac ggaggctgat gtggaatcac tgactaagag
     6301 atctggcttt gttgcgaacg gcaatgcaag gatttattat gaaaccgctg gcgcggggtc
     6361 gccctttgtc atgatacacg ctggtgtggc cgatcaccgt caatggaata acgaattcgc
     6421 ctctttcgca acgaaccacc acgtcgtccg gtatgatatg cgcgggtatg ggaagagtga
     6481 gccggtcgag ggtgaattca atcccatgga tgatctggtt tcagtactcg acgtgcttga
     6541 acttcatgaa ccgattgtcc ttatgggctg ttcaatgggc ggcggcttag ccatggattt
     6601 tgcgctcaag tatccagccc gcgtaaaagc gttgatcatg gttggctcag gtccaagcgg
     6661 actggaattg gatgtccctg cacccgccaa atttgcagaa gcagagaagg cctatgagtc
     6721 cggcgatctg gatctcctgg ctgaaatcga aacgcaaatc tggttcgatg gcacaggccg
     6781 atcacccgaa caagtggatc aaaccatgcg caacctcgcc tatgaaatga atcgcctcgc
     6841 tcttgcacat gagtccaaac aactcggcaa gcgtctgccc aatgcagaga ccccggcatt
     6901 cgatcgactt gctgacctaa agattcctgt cctgatcatc gttggagagc atgacactcc
     6961 ctatatcctt gcggcggcag actatatgct acaaaatata ccttctgctc gcagggtatt
     7021 aatcgaggat gccgcccatt tgccgaatat ggaccatccg gaggagttcg aaaacgcggt
     7081 gaaggacttt ttggatcgtc tttcgcattg attgttgaag cgctgtctgg caatcgcttt
     7141 tcaccaaact tg
//
LOCUS       DASTJP010000191         6288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_592439, whole genome shotgun sequence.
ACCESSION   DASTJP010000191 DASTJP010000000
VERSION     DASTJP010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592439"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFQ23_05830"
     CDS             complement(<1..434)
                     /locus_tag="VFQ23_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9906135.1"
                     /translation="MHPGFVKPRYDSGGFAGIPDRIKESFASDNYDAVVLFLVDGFGW
                     RFFERFQDAPFIKRFARNGRIEKLTSQFPSTTAAHLTTIHTGWNVGQSGVYEWYYYEP
                     QLDAIISPLLFSYSGSEYRENLKSTGINPQLLFPRGILYPEL"
     gene            complement(434..1729)
                     /locus_tag="VFQ23_05835"
     CDS             complement(434..1729)
                     /locus_tag="VFQ23_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9906136.1"
                     /translation="MKIAIIGAGFGGMAAAYDLRKAGHEVIIYEAANYVGGLASGFKE
                     PHWDWSVEKFYHHWFQSDSSMLGLIEELGLKYKVIFPRPYTVLLHNGKWYPFDSILKA
                     LLFPGLGFGLDKIRFGFVGLYLRLTNDWKNLEQYTADEWMRKYAGSKVYEKMWKPLLV
                     GKFGPFYKDVNMAWMWARLKARTTRLGTFEGGFQKFADLFAEKLRHMGVEIRLGAQVK
                     FIKQSPDKGLLIDAGGAVESFDKVLVTTSPSLLAKFAPDLPENYLKGLLELKSMGAVV
                     MVLSLKHQLSREGYYWFSLPKDAGYPMLALVEHTNFVSKEHFGGDHLVYAGDYLEAGH
                     EYFSMSDEELLERFTPALKKFNSAFSRDWVKKIWVNKTNYAQPVPLVNHSKNIPAIQT
                     PIEGLYFASMSQVYPWDRGTNFAVEIGRRAARMMLGEAR"
     gene            complement(1915..2565)
                     /locus_tag="VFQ23_05840"
     CDS             complement(1915..2565)
                     /locus_tag="VFQ23_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9906137.1"
                     /translation="MTQPISQKRKLRVLIADDVQETRRNTRLMLATIDNVEVVAIAAN
                     GLQAVQLAKEHHPDIVLLDINMPEMDGLTAFRNIVQIHPDIGCIILSAEKDTTTLRTA
                     ISLGVQEYLIKPFTIDELEVALARVNDKVDETRVKLAQVHDLKKKKEDYLKQLATEYS
                     KARRTDEKAMEVFEQLADYPNCDARWLQTLAMIYVVRQEWGRLRILSTRLEQRNKK"
     gene            2814..3743
                     /locus_tag="VFQ23_05845"
     CDS             2814..3743
                     /locus_tag="VFQ23_05845"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET9906138.1"
                     /translation="MAEKQSRKLKDPESQKQLAEAKAYFGSNPLLASPPVKRAAYSDR
                     MAWVLASMSHLAYDRFEDGGKTKALLLKKLRGGGFKWIDGFSSDRTGTQAFLVIKDDR
                     SYAVLAFRGTEQTWRDIQTDIRARRIRTPKGKIHLGFKDAFSSVEEEIKKRLESLSDI
                     PLYITGHSLGGALATVATQILEWDSRFADQIAACYTFGSPRVGNDEYDSSIKSMFYRV
                     VNTTDVVTIVPLLMMGFVHVGDVRFLLRNMGEFKRSIPFSRRLFFFLGAGMLKFFRPL
                     VEDHAILEYRKKLEAVAQDRNLNLYFDISSEAK"
     gene            complement(3777..4448)
                     /locus_tag="VFQ23_05850"
     CDS             complement(3777..4448)
                     /locus_tag="VFQ23_05850"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9906139.1"
                     /translation="MPLDLSRVKALCFDVDGTLSDTDELYSIRIARFLPRFLFRDPAH
                     TARRLVMWAEAPGNALLGMADTLGLDDAMTAVIDWISRHRKFTDDELLLIPGVDVLLS
                     RLHGRYPLAVVSARDEKATMRFLERFDLVKFFDVIVTGLSARHTKPYPDPILFAAQKM
                     GVKPEECLMIGDTTVDMRAGKSAAAQTVGVLCGFGEEEELLRLGADYIVKNTADLADL
                     LLDKK"
     gene            4543..>6288
                     /locus_tag="VFQ23_05855"
     CDS             4543..>6288
                     /locus_tag="VFQ23_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9906140.1"
                     /translation="MSLQQEQDRTKAQLELLYHVSRQVATALDLRTVLQRVLYEAIQN
                     VGGERCSVIVLDDAAKAVDATIVYGTQVHEHTTQQMRDTMERGLAGWVTRHRKGALVL
                     DTSKDERWVQRPDDAADRSGAKSAICVPLQARENLVGVLTLVHPIPNSFREEQLDLMQ
                     AIADQAGVAILNARLYTESQRQARVMTALAEGAAAMNVSLRMEDVYQRTLIQMMQALQ
                     VETVALGMLDGEHIIFRAAAGHNAGNILGRKIPLGEGIAGNAAREGRGVVVQDVSHDK
                     LYSDADKFGGVEMRAILVAPIQAQGRVIGVLEAINPIAKTFDPDALLVMTGIGGLAGT
                     TIQNAQLFERLQAAHQRYRELFDDSIDPMLITDWDGHILEANRQAVSLSGYSDEALRN
                     LSIVQLHDVNWNKTGKKFNTLRLDSTCSYESLLHKQDDSQTPIEVHARRVEFDETDSI
                     QWILRDITERKELDGLREDLTSMIYHDLRSPLGNIVSSLDVLYSMVPEEDKETVLSIL
                     KIAENSTDRIQRLVSSLLDVNRLESGQPVADQKVVDPLLLIKNAIRDVEPVAKGRRQT
                     FILDLPEEMPSIWVDE"
BASE COUNT         1625 a         1539 c         1521 g         1603 t
ORIGIN      
        1 ctcagttcag gatacaggat cccgcgtggg aacaaaagtt gaggattgat acctgtggac
       61 tttaaatttt ctcggtattc agaaccggaa tacgaaaata gcagtggaga aataattgca
      121 tccagttgag gttcatagta ataccattca tatacgccgc tctgaccgac attccagccg
      181 gtatgaatgg tggttaaatg cgctgcggtc gtggatggga attgcgaagt tagcttttcg
      241 atcctgccat tccttgcaaa acgtttgatg aacggggcat cctgaaagcg ttcaaagaac
      301 cgccaaccga atccgtctac taaaaacaga acgacagcat catagttatc tgaggcaaac
      361 gactctttta ttcggtcagg tattccagcg aatcctccag agtcataacg aggctttacg
      421 aaacctggat gcattatctc gcttccccga gcatcatcct tgccgcgcgt cgcccaatct
      481 ccactgcaaa gtttgtgccg cgatcccatg gatagacctg gctcatcgaa gcaaaatata
      541 atccttcaat tggcgtttgg atggcgggga tattctttga atggttgacc agcggcaccg
      601 gctgagcata attcgtctta tttacccaga ttttcttgac ccaatcgcgc gaaaacgcag
      661 agttaaattt cttcagcgcc ggtgtaaagc gttccaaaag ttcctcatcg ctcatcgaaa
      721 aatattcatg tcctgcctct aaatagtcgc ccgcgtacac aaggtgatcg ccgccgaaat
      781 gttccttcga gacgaaattc gtatgttcca ctaaagccag catcggatag cctgcatcct
      841 tgggcaggct gaaccaataa tatccctcgc gggacaactg atgtttgagc gataacacca
      901 ttaccactgc gcccatagac tttaactcaa gtaacccttt tagataattt tcgggaagat
      961 caggtgcgaa ctttgccaaa agacttgggg aggtggtgac tagtacctta tcgaaagact
     1021 cgacagcccc acccgcatcg atcaatagac ctttatcggg actctgctta ataaacttga
     1081 cctgtgctcc gagccgaatc tcgactccca tatggcggag cttttctgca aagagatcgg
     1141 cgaacttttg gaagccgcct tcgaatgttc ccaggcgtgt tgtgcgggcc tttaagcggg
     1201 cccacatcca tgccatattg acatctttat aaaatgggcc aaacttcccg accagcaagg
     1261 gcttccacat tttttcatag actttgctac ctgcgtactt tcgcatccac tcatctgcag
     1321 tatattgttc gaggtttttc cagtcgttcg ttaaacggag atagagcccg acgaagccaa
     1381 accggatctt atcgaggcca aatccgagcc cgggaaagag caatgcctta aggatggagt
     1441 caaacggata ccactttccg ttatgtagta agaccgtata tggacgtggg aagattactt
     1501 tatacttcag ccccagctct tcaattaacc ccagcatgga gctatctgat tgaaaccagt
     1561 gatggtagaa cttttctacg gaccaatccc agtggggttc cttgaatcca ctcgccaagc
     1621 caccaacgta atttgcagct tcataaataa taacttcatg cccagcctta cgcaggtcat
     1681 aagcagccgc catgccgcca aagcccgcgc caataatcgc gattttcatt catgctccaa
     1741 ataatagaat tgtcattgcg aagagggtcg acacttgcac ttgcatgcaa gtgcaggtga
     1801 gacataacga agcaatctcc acatactttg gtaattttta ttatgggaga ttgtttcacc
     1861 gcctcggtct cgatacgggc ttcgcgacca gcggcggtcg caatgacata acctttactt
     1921 tttgttccgc tgttccagcc ttgttgaaag gattctcagc ctgccccatt cctgccggac
     1981 tacataaatc atagccaaag tctgcagcca gcgtgcatcg caatttggat aatcagccaa
     2041 ctgctcgaat acttccattg ccttttcgtc agtgcgcctg gctttcgagt attccgttgc
     2101 gagttgcttt aggtagtctt ctttcttctt ttttaggtca tggacctgcg ccaatttaac
     2161 gcgggtttca tccactttat cattgacgcg ggccagcgca acttctaatt cgtctatggt
     2221 aaaaggcttg atcagatact cctgcacacc cagagagatg gcggttcgca gggttgttgt
     2281 atctttctcg gctgagagaa taatacagcc gatatccgga tggatctgta cgatattccg
     2341 aaatgctgtc aatccatcca tttctggcat attgatatcc agtaggacga tatccggatg
     2401 atgttccttt gccagctgga cagcctgaag tccattggcg gcgatagcga ccacctcgac
     2461 attgtcgatg gtggccagca tcaagcgagt gttgcggcgg gtttcctgaa catcatcagc
     2521 gatcagtaca cgcaattttc ttttttggga tatgggctgt gtcatttgaa taccttcaac
     2581 ttactcaatg cagcgccagc ttcacggtat cttaagtata accgaggctc gctttagcag
     2641 ggggaatagt cagcctaact tgggttgaaa tgtaccctgc caggcgctga aaatgtccca
     2701 gagagccata ggagtagcga aaattgcgtg tcaaaataaa gaaacatcct gagttgactc
     2761 tcaattaaaa tttctgtata gtaggatcat ccatgacaga gcggagagga cagatggctg
     2821 agaaacaatc cagaaaattg aaggatccgg aaagtcaaaa gcaacttgcg gaggcaaaag
     2881 cttactttgg gtctaatcca ctcttggcta gcccgccggt aaaacgcgct gcctattcag
     2941 atcgtatggc ctgggtgctc gcgtccatgt cccatttggc ctatgacaga tttgaagatg
     3001 gtggcaaaac aaaggcattg ttgctcaaga agctcagggg tggggggttc aagtggatag
     3061 atggattctc ttcagataga acaggaacac aggcattttt agtcatcaag gatgatcgtt
     3121 cctacgccgt cctggctttt cgaggcaccg aacagacctg gcgtgatatc cagacggata
     3181 tacgcgctcg acgaatccgg actcccaagg gcaaaatcca tctggggttc aaagacgcat
     3241 tctcaagtgt cgaagaagaa attaagaagc gtctggagtc cctatctgat attcccctat
     3301 atataaccgg tcattcactc ggaggcgcgc ttgctacggt tgcgactcaa attctcgaat
     3361 gggattcgcg ttttgccgat cagatcgcgg cttgttatac gttcggaagc cctcgagttg
     3421 ggaatgatga atatgatagt agcatcaaat ccatgttcta tcgtgtcgta aataccacgg
     3481 atgttgtcac aattgttcca ctcttgatga tggggtttgt acacgtcggt gatgttcggt
     3541 ttctcttacg taatatggga gagttcaagc gaagtatccc attctcccgc cggttattct
     3601 tttttctggg tgcgggcatg cttaaattct ttcggcccct ggtggaagac cacgctattt
     3661 tggaatatag aaaaaagctg gaagctgtcg cccaggaccg caacctgaat ttgtattttg
     3721 atatttcatc tgaagctaag tagttttggt aatctgaatg ttattcgcga gacgtattac
     3781 tttttatcaa gaagtagatc tgccaggtcc gccgtatttt tcacaatata atctgctccg
     3841 agcctgagca attcttcttc ctcgccaaag ccacatagca cgcctaccgt ctgtgccgca
     3901 gccgacttgc ccgcgcgcat atctacagtc gtatcgccaa tcatcagaca ttcttcgggt
     3961 ttgacgccca ttttctgcgc ggcaaagaga attgggtctg gataaggttt cgtatgccgt
     4021 gcagaaagac ctgtaacaat cacatcgaaa aatttcacca aatcaaaccg ctcaagaaag
     4081 cgcattgtcg ctttttcatc ccgggcgctg accactgcta atgggtaacg gccgtgtagc
     4141 cggcttaaca acacatctac accggggatc agaagaagct catcgtccgt aaatttccgg
     4201 tgtcgcgaga tccagtcaat tacagccgtc atcgcatcat ccagccccaa tgtgtctgcc
     4261 attcccagta gtgcattgcc aggcgcttct gcccacatca ccagccttct ggcagtatga
     4321 gcagggtcac gaaagagaaa acgcggcaga aaacgtgcaa ttcggattga ataaagttca
     4381 tccgtgtcac ttaacgtccc gtccacatca aaacagaggg ctttgacacg ggagaggtca
     4441 agtggcatga gacaattgta ctctcagtgc cttcaaactg aatatgctat actctattta
     4501 tctgggcgtg gtatcatcta taaaggaatt tgactggaat ttatgtcact tcagcaggaa
     4561 caagaccgaa ccaaagcaca gctggaactt ctttatcatg tgagccgcca ggtagccacc
     4621 gcgcttgacc tgcgtactgt acttcagcgt gtgttatatg aagccattca gaatgtgggt
     4681 ggagaacgtt gtagtgtaat cgtcctggac gatgcagcca aagcggtaga tgccaccata
     4741 gtgtatggca cacaagtcca tgaacacact acccagcaaa tgcgcgatac gatggaacga
     4801 gggctggcag gctgggtcac gcgccatcgt aaaggtgctc tggttctgga tacaagcaag
     4861 gatgaacgat gggtccaacg acctgacgat gcagcagata gatcgggcgc gaaatcagca
     4921 atctgtgtgc cgttacaggc gcgcgaaaat ttagtgggtg tgctcaccct tgtgcacccg
     4981 attcccaatt catttcgcga agaacaactt gacttaatgc aggccatcgc agatcaggcc
     5041 ggggttgcga ttctcaacgc acggctctat acagagagtc aacggcaggc gcgggtcatg
     5101 acagctctgg ctgaaggcgc ggcagcgatg aacgtgtcgc tacggatgga agatgtgtac
     5161 cagcgcactc tgattcaaat gatgcaagcc ctgcaagtgg aaaccgttgc gcttggcatg
     5221 ctcgatggcg agcatattat cttccgagcg gctgccggtc ataatgcagg taacatcctg
     5281 gggcgcaaaa ttccactcgg agagggaatt gccgggaatg cggcacgtga aggtcgtggc
     5341 gtggttgttc aagacgtgag tcatgataag ctctatagtg atgccgataa attcggtggc
     5401 gtcgagatgc gggcaattct tgtggcacca attcaggcac agggacgcgt catcggtgtt
     5461 ctcgaagcga tcaacccaat tgccaaaacc tttgaccccg atgcactgct ggtcatgacc
     5521 ggtatcggag gtctggctgg taccacgatc caaaacgcgc agctatttga acgcctgcaa
     5581 gccgcacacc agcgctaccg cgagttgttt gatgatagca ttgacccgat gttaatcacc
     5641 gattgggatg gtcatatcct tgaggcaaat cgtcaggctg tttcgctaag tggttactca
     5701 gatgaagcac tacgaaatct ctccattgtt cagcttcatg atgtcaattg gaacaagacg
     5761 gggaaaaagt ttaacaccct ccgtttagac agcacttgtt cctatgagtc attgctgcat
     5821 aaacaggatg attcacaaac tccgattgaa gttcatgcgc ggcgcgtgga atttgatgaa
     5881 actgattcga tccagtggat tctacgcgac attacagaac gcaaagaact ggatggcttg
     5941 cgtgaagatc tgacctccat gatttaccat gatctgcgct cgccacttgg gaatatcgtt
     6001 tccagtctgg atgtacttta cagtatggtg ccggaggagg acaaggaaac cgtcctttcc
     6061 atcctcaaaa ttgccgaaaa ctcaacagat cggattcaac gtctggtcag ttctctctta
     6121 gatgtcaacc gactagagtc gggtcagcct gtagcagacc aaaaggtagt tgacccactt
     6181 ctcctgatca agaatgccat ccgcgatgtg gagccggttg caaaagggag acgtcagacg
     6241 ttcattttag atctccctga agaaatgcca tccatttggg tggatgaa
//
LOCUS       DASTJP010000192         8303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_353793, whole genome shotgun sequence.
ACCESSION   DASTJP010000192 DASTJP010000000
VERSION     DASTJP010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353793"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFQ23_05860"
     CDS             complement(<1..282)
                     /locus_tag="VFQ23_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9906141.1"
                     /translation="MLEVNGLSAGYQGKDVVHNIAFHLNKGEAVMIIGSNGAGKTTLF
                     QALCGLLAPSSGKVIFEGKDITGMPAERIARLGMRFVSAARHLFPRMTVD"
     gene            complement(275..1015)
                     /locus_tag="VFQ23_05865"
     CDS             complement(275..1015)
                     /locus_tag="VFQ23_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906142.1"
                     /translation="MALLELDKVEKHFGGLAAVSGVTMKADDGEIVAIVGPNGAGKST
                     MLKLIVGLELPTSGSIHFAGANITRFPAHRIRQMGIAMVQQTPRHFSTMTVLENAALG
                     GLFGKAGAYRKEAQALELAREALSLVGLEDRSHDSVGNLNLHEQRFLELARAIAGQPR
                     LLVLDEVMAGLNDGELEASIKTIRTIRDRFNITILWVEHVMKAVMNLAERTIVLNFGK
                     VLTEGLPKDVMRHPSVIEAYLGKEIKHA"
     gene            complement(1024..2331)
                     /locus_tag="VFQ23_05870"
     CDS             complement(1024..2331)
                     /locus_tag="VFQ23_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9906143.1"
                     /translation="MNKKKGITLKIIPALLVLTLVLTACGAATPTETAAPATEPPATE
                     MPATEAPATEAPATEAPTEPPSTEPIKIGMLTDLTGFTPWAVQVRDGMALAAQEINDA
                     GGVNGRMIELVTQDSENNAEVGVDRFERLVEEDVVAVGGIISSAVGAPVGAAAEVEQV
                     PVFLVKSGTPAALTRASRYTFRTCLPSAPMVAAPVLQYAQEQGFTKVGGIVADYPWGQ
                     AFKGAMEGAFEGSGIDYTIEVAPVPPDTDFTPFVRAMADFGAEMIVATGHPPGNSAIV
                     TLSADLAGNVPVTGAWIPPDLAVGGLQDLAIGRYSDFACADYLGDSYAELAQRYLAFS
                     DNLFMSDDAVAGYAIVQIVAEAVGAVGDDRAAIAEYVRTNEFDMPGYAHPLSWTEWGE
                     LASSAPIFFRISEGPAPEGLNEAGDWWLELLTHSATLTPYEPE"
     gene            3378..4457
                     /locus_tag="VFQ23_05875"
     CDS             3378..4457
                     /locus_tag="VFQ23_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9906144.1"
                     /translation="MKKSLLYVLSLVVVASMVLTACGGAPAATEPPAAEPQFTCALIT
                     PNPLGDRSFIDASARGIEQANAELPVNCDIIETNGVAEHETALRGAIANNYDLVLGLA
                     MDTEMFVSLAEEFPDQKFASPSEIFLDALPPNMVAYQIDVHESSFLVGLIVGSLTQTK
                     TVGAVVGGDAPGLNQFYYGYKQGVLEVCPDCEVLVSYLGFDFSNPTLGLETALGLYDQ
                     GADIIYQVAGRSGEGVLEAAAQRDLYAIGVDSNQDDIQPGHVIVSSLKKVESTTFDPI
                     KLVVDGTFTGGFQKRGLKEGFAGLSWDEGSTTFEDQGPAELTALIPEIKTLVEEYRAK
                     ILSGEYKVCDAFVADSPECVALLAQ"
     gene            4527..6086
                     /locus_tag="VFQ23_05880"
     CDS             4527..6086
                     /locus_tag="VFQ23_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906145.1"
                     /translation="MTPAVEMKGITKFFASTQVRANEGVDFSVEHGEVHALVGENGAG
                     KTTLMNILYGLIKPDSGEIIIDGEKVRIDHPDEAIKHGIGMVHQHFKLVPSFTVAQNI
                     LLGMEPTQTGFINQQKENELVDELAKKFNLPVDPAARIWDLPVSMQQRVEILKVLERR
                     AKILILDEPTAVLTPQEVNELITVIRGLSKQGYTIIFITHKLIEVMTVADRVSVMRGG
                     RMMGTKKVSETSIPELASMMVGREVLLRVKKKEMQRGEVVLEAKDISIADVNGLAAIS
                     HVSFNVHKGEIVGIAGVSGNGQTELVEAISGMNNLDGGEIVFRGKTINEQSVGQRRDM
                     GMAHIPEDRMVVGLNLQTTLDENLIVSRYDHSEFSRYGFLLMKSINEFAQKVVNAFSI
                     AAARPGEGISMLSGGNLQKVVVGRELSGDPSFIIANQPTRGLDVGSIEFVHKTLIEER
                     DKGAGVLLVSVELDEIMALSDRILVLYRGRIQGELDAATATEEEVGILMAGGTLKDLP
                     HRSDNKREVMS"
     gene            6083..7183
                     /locus_tag="VFQ23_05885"
     CDS             6083..7183
                     /locus_tag="VFQ23_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906146.1"
                     /translation="MREQRLRKLLDAILVPLLAVISGLVVMGILILILRKPLFESYAV
                     LFSKGFGCAAYGNCIFITALERATPLILTGLAAVVAFRSGMFSIGQEGQYLLGAAAAA
                     WLGYVIHLPPVIHALVLIIAAMLVGAAYGWIPGVLKVRLGVNELLATIMLNAIAVLVV
                     EYLVQFPMRADTSAVAHSPVIDETAQLMSFLPGSKWGVGFVIAVITTLAVYFYLWRTK
                     AGYEQRMAGQSRKFALFGGIPSDNSAIRAMLISGALAGLAGAIEVMGVHRRITTGFSV
                     GLGFDGLTSAILGQVHPFGVFIVAILFSGLKLGAQLGLQLNLDIPRELGGTIIGFIIL
                     FVAAGKLFEENIARAREWFARRRSSTVKGESP"
     gene            7185..8165
                     /locus_tag="VFQ23_05890"
     CDS             7185..8165
                     /locus_tag="VFQ23_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906147.1"
                     /translation="MEGFFSVFTVELLNSGIRLATPILLAALGGAICNQAGVLNLALE
                     AKMLLGAFVGIVAAYYLGNSYGGLLVAMIAGGLLGLLFAFLYHKYRVDLVILAIAFNL
                     IVVELTVFVMRAMFGNVGSWSDPSIERIPDIQIPLLKDIPFLGPLLSGYNIIVYFSWL
                     VVIVMFVYLYRMRGGRHLRAVGENKSAAETVGINAILLQTLALVISGALAALGGAFLS
                     IGHLKLFTRNMSNGRGWIAIAAAMFGVNHPIGVFLASLFFGFADAFSVRIQNVTDLPP
                     NLVKLLPNVATLLVLIIIALRGMIAVGVARRRFRAQLRAEETAAPVQSSS"
BASE COUNT         1920 a         2056 c         2200 g         2127 t
ORIGIN      
        1 gtcgaccgtc atacggggaa acagatgacg cgcggcggac acaaacctca ttcctaatcg
       61 cgcaattcgc tctgccggca tcccggtgat gtctttgcct tcaaagatca ccttcccgga
      121 agaaggcgcg agcagaccac acaacgcctg gaacagtgtc gttttcccgg caccattgga
      181 gccaatgatc attactgcct cgcctttgtt gaggtggaaa gcgatgttgt gaacgacatc
      241 tttgccctgg tatcccgctg aaaggccgtt aacctcaagc atgtttgatc tccttaccca
      301 ggtaggcctc gatgactgac ggatgacgca ttacatcttt tgggagacct tcagttagta
      361 cctttccaaa gttaagaact attgtccgct ctgccaggtt catgacggct ttcattacgt
      421 gttccaccca gagaatagtt atgttgaacc gatcacggat cgtccggatt gttttgatgg
      481 aagcctccag ctcaccatcg ttgagccctg ccattacctc atccagtaca aggagtcgcg
      541 gttgccctgc gatggcccgt gccaattcaa gaaaccgctg ttcatgcagg ttgagattac
      601 ccaccgagtc atgactccgg tcttccaatc cgactaacga cagtgcctct ctggcaagct
      661 ccagagcctg cgcctccttg cggtatgctc ctgcctttcc aaagagccct cccagggccg
      721 cattttcaag taccgtcatc gttgaaaaat gtctgggagt ctgttgcacc atcgcgattc
      781 ccatctgacg aatgcggtgg gcggggaacc gggtgatatt cgcgccagca aaatggatgc
      841 tgccgctggt tggcaattcg agaccaacaa tcagtttgag catagtgctt ttgccggccc
      901 cattggggcc gacgatggcc acaatctctc catcgtcggc cttcatggtg acgccactga
      961 cagcggcgag accgccaaag tgtttttcga ccttatccag ttctaagagc gccactgggg
     1021 atattactct ggttcatagg gtgtgagcgt ggcggagtgg gtcagcaatt ccaaccacca
     1081 gtcgccggct tcatttaacc cttcgggtgc cggtccttcg ctgatgcgga agaaaattgg
     1141 agcagacgaa gccaactcgc cccattccgt ccagctcagc gggtgggcat aacccggcat
     1201 gtcgaactcg ttggtacgta catattcagc gatagccgca cgatcgtcgc caactgcgcc
     1261 cacagcctct gcgacaatct gtacgatcgc atacccggcg acggcatcat ctgacatgaa
     1321 caggttatcg gagaatgcca gataccgctg cgctagttca gcatagctgt caccgaggta
     1381 atcggcgcag gcaaaatcgc tgtagcgtcc gatggcaaga tcctgcaacc ctccaaccgc
     1441 caggtcgggc ggaatccatg cacctgtaac gggaacgttc ccggctaagt cggcggacag
     1501 tgtcacgatc gctgagttac caggtggatg acctgtagcc acgatcattt cagcaccaaa
     1561 atccgccatc gccctcacga acggggtgaa atccgtgtct ggtggcaccg gtgcgacttc
     1621 gattgtgtaa tctatgcccg aaccctcgaa tgcaccttcc atcgctccct tgaaggcctg
     1681 tccccatggg tagtcagcaa caatgccccc caccttcgtg aatccctgct cctgtgcgta
     1741 ctgcagcacc ggcgctgcga ccataggtgc agatggaagg caggtgcgga aggtgtatcg
     1801 gctggcacgg gtaagggctg ccggggtacc agacttcacc aggaaaactg gaacttgttc
     1861 cacctcagca gccgctccga cgggcgcgcc gaccgcactt gaaataatgc cgccgactgc
     1921 cactacatct tcttcgacaa gtcgctcgaa gcggtcaaca cccacttcgg cattgttctc
     1981 tgaatcctga gtgacgagct cgatcatgcg gccgttaaca ccgccagcat cattgatttc
     2041 ctgtgcagca agagccattc catcgcgaac ttgaactgcc caaggcgtga agccggtcag
     2101 gtcggtcagc attccaatct tgatcggttc tgtggatggg ggttcggtgg gcgcttcggt
     2161 cgcgggagcc tcggtcgccg gagcttctgt agcaggcatc tccgttgcgg gcggttccgt
     2221 ggcaggcgcg gctgtttccg taggtgtagc cgctccacac gctgtcagga ccagcgtgag
     2281 caccagcagc gcagggatga tcttcagggt gattcctttt ttcttgttca ttcttcctcc
     2341 aatttgtaaa tgaatatccg aggatgcaaa taagaatctt cactattgtg ccagttcaca
     2401 ctcctttcgc tgcatagcat caattggcag gaatttcctg ccaattgatg catgtttgaa
     2461 aattaaagtc aatgaccttc aaggctctga cacggtgcta ccggtgttcc atggcaatga
     2521 aatgttttca tgagttgggg tcaggaagag agtccagaga ggaaggttca tacacgcagc
     2581 ctccaagtat ggttagtatc acatagaatg caaacgcgga caagaggatt gcaattaaaa
     2641 ttaatttgta tgagtaaaaa ccatcagatc agaatgtatt ggtgtgaaac gttgaaggat
     2701 atctaagatt caaactcatc tttttcatta catttcctta ctcaatgaag gtatcaccag
     2761 atcggtggtc tggcaaaact ggacaaaagt gtgagggttg tcagaatacg tgagaattgg
     2821 tttttagtaa aagataatca cacaaaagcc gcgtagatct ttcttctttt tgatccaggt
     2881 tcatattttc tctcttttga ttcattgtat aaacactaac agcgcgagga attttcacct
     2941 catcttctgt gggggaactc gatgacaata tcacaagaat ttctagactt aaccgaccga
     3001 ttacagatcg gctcgactcg catcttatct cccatccccg tgggggcacg tacgaaaaga
     3061 tatgcctgta gtcatgtctt tttatttgtg ggacatgata ccaagccatt taacgttaga
     3121 tcataaaact ctaaagagag gctggtttct cgcaatcagg ctaaaatgct acacatactt
     3181 tccattttat gtataatcgt tcaaagacga acagattgaa caatatgcct ctttcgagaa
     3241 gtgagagtcc ggcaaaggcc tatgtacaaa cggtctatgc agggattcca tttttttaag
     3301 ggaaggaggt gattacgcca atttttcata aatctgtttg cgcacatccc aattaatcaa
     3361 caataggaga agaagagatg aagaaaagtt tgctttatgt tttgagcctt gtggtggttg
     3421 cttccatggt actcacggcc tgtggtggcg cgcctgccgc gactgaacct cccgccgccg
     3481 aaccgcaatt cacctgtgca ttgatcaccc ctaatccgtt gggcgaccgg tcattcattg
     3541 acgcctcggc acgtggtatc gaacaggcga atgcagaatt accagtcaat tgcgacatta
     3601 ttgaaaccaa cggtgttgcc gagcacgaga ctgctttgcg aggcgcaatc gcaaataact
     3661 atgacctggt gcttggcctg gcgatggata cggagatgtt tgtcagcctt gcggaggaat
     3721 tcccagatca gaagttcgcc tcgccgtctg aaatcttcct ggatgctctg cctccaaata
     3781 tggtcgcgta tcaaattgat gttcatgaat ccagcttcct ggtgggtttg atcgtgggtt
     3841 cgctcaccca gaccaagaca gtgggcgcgg ttgtgggtgg agatgcccct ggcctgaatc
     3901 agttctacta tgggtataag caaggtgtgt tggaagtctg cccggactgt gaagtgttag
     3961 tcagctatct tgggttcgat ttctccaacc cgaccctcgg gcttgagacg gctctcggtc
     4021 tttatgacca gggcgccgat atcatttatc aagtggcagg ccgatctggt gaaggcgtgc
     4081 tcgaagctgc cgcgcagcgt gatctgtatg ccattggcgt cgactcaaat caggatgata
     4141 ttcagcccgg acatgtcatt gtttcgagtc tcaagaaagt ggaatccacg acgtttgacc
     4201 cgatcaaact ggttgtggat ggcaccttta ctggcggctt ccagaagcgt ggtctgaagg
     4261 aaggtttcgc cggtctttca tgggatgaag gctcaactac gtttgaagat cagggtccgg
     4321 ccgaactgac tgcgttgatc cccgaaatca aaacgctggt cgaagaatat cgtgccaaga
     4381 tcctcagtgg cgaatacaaa gtttgtgatg cgtttgtggc tgattctccc gaatgtgtcg
     4441 cactgctcgc tcaataattt gaagatcatt gtttgaaaat agactccggg catgaacgcc
     4501 cggagtctaa tccaaaggat gtgccaatga cgcctgcggt cgagatgaag ggcatcacca
     4561 aattttttgc atccacacaa gtgcgtgcca atgaaggcgt tgatttttcg gtggaacatg
     4621 gggaagtgca cgcgctggtt ggtgagaacg gcgctggcaa aacaacgctg atgaatatcc
     4681 tttacggttt gatcaaaccg gattccggtg aaattatcat cgatggagaa aaggtccgca
     4741 tcgatcatcc tgatgaagcc atcaaacatg gtatcggcat ggttcaccag cacttcaagc
     4801 tggtaccttc ctttacagtc gctcagaata tcctactcgg aatggaaccg acacaaaccg
     4861 gtttcataaa ccagcagaag gaaaatgaac tggtcgacga attagccaaa aagttcaacc
     4921 tgccggttga ccctgctgcc cgtatctggg acttgccggt aagcatgcaa cagcgtgttg
     4981 aaattctgaa ggtattagag cgccgcgcaa aaatattgat tttggatgag cctactgccg
     5041 tgctcacgcc tcaggaagtc aacgaactga tcacggtcat tcgcggactc tccaaacaag
     5101 ggtacaccat catcttcatt acacataaat tgattgaagt catgacggtt gcagatcgag
     5161 tcagtgtgat gcggggtggc agaatgatgg gcacaaaaaa agtttctgag acgtccatcc
     5221 ctgaactggc cagcatgatg gtgggtcgtg aagttttgct tcgagtaaaa aagaaggaaa
     5281 tgcagcgagg ggaagttgta ctcgaggcta aagacattag tattgcagac gtcaacgggc
     5341 tggcagcaat ttcacatgtt tcttttaatg ttcacaaggg tgaaattgtc ggtatcgccg
     5401 gtgtcagtgg caacggtcag acggaacttg tcgaagcaat ctctggcatg aacaacctgg
     5461 acggtggtga gatcgtcttc cgaggaaaga caatcaacga acaatccgtc ggtcaacgac
     5521 gcgatatggg tatggctcat atccctgaag accgcatggt ggttggatta aatcttcaaa
     5581 ccacgctgga tgaaaattta atcgtctcac ggtatgacca ctccgagttc agtcgttacg
     5641 gatttctgtt gatgaagtcc attaacgaat ttgctcaaaa agtggtgaac gctttttcga
     5701 ttgcggccgc gcggccgggg gaaggcatct cgatgctttc cggcggcaat ttacagaaag
     5761 tcgttgtcgg tcgtgagcta tcaggagacc cttcttttat tattgccaac cagcctacac
     5821 gaggactgga tgtgggcagt atcgaatttg tgcataagac tttaattgag gaacgcgata
     5881 aaggcgctgg cgtcctgctc gtttccgttg aactggatga gatcatggcg ttatcagacc
     5941 gcatcctggt gttgtatcgc ggcaggattc aaggcgaact tgacgctgct acggccaccg
     6001 aagaggaagt gggtatcttg atggcaggtg gaacgttgaa agacctaccg caccgatccg
     6061 ataataaacg ggaggtgatg tcatgagaga gcaacggctc agaaaactgt tggatgccat
     6121 tcttgtgcct ctccttgcag tcatctctgg tctggttgtg atggggattc ttattctgat
     6181 tttaaggaaa cctctttttg aatcctatgc cgttcttttt tcgaaagggt ttggctgtgc
     6241 ggcgtatggc aactgcattt ttatcacagc gctggaacgt gcaacccctc ttatcctcac
     6301 cggcttagcc gcggtcgtcg cattccgctc ggggatgttt agcatcgggc aggagggtca
     6361 gtatttgctg ggagctgcag cggctgcctg gctggggtat gtgattcatc tgcctcccgt
     6421 gatccatgct cttgtattga tcatcgctgc catgctggtc ggcgcagcct atggctggat
     6481 ccccggagtt ctcaaggtcc ggctgggtgt gaacgagctg ttggcaacca tcatgctaaa
     6541 tgctattgcc gttctggtgg ttgaatacct ggtgcaattc cccatgcgcg cggacacaag
     6601 cgctgtggcg cattcgccgg tgattgacga gactgcccaa ttgatgtcat ttctgccagg
     6661 gtcaaaatgg ggcgtgggct ttgtcattgc ggtcattaca accttggctg tgtattttta
     6721 tctgtggcgc acgaaagccg gttatgaaca acgtatggcc gggcaatcaa gaaagtttgc
     6781 actgtttgga ggcatcccca gcgataactc tgccatccgc gccatgctga tcagcggagc
     6841 actcgccggg ctggcgggcg ccattgaggt gatgggggtg catcgccgca tcacgaccgg
     6901 cttttcggtc gggttaggtt ttgacggtct tacatctgct attctgggac aggtgcatcc
     6961 gtttggtgtg ttcatcgtgg caatcctgtt ctcaggcctt aagctgggcg cacaacttgg
     7021 tctgcaattg aatctcgata tcccgcgcga gctgggtggt acgatcatcg ggttcattat
     7081 tctcttcgtt gcagccggga aactatttga agagaatatc gcccgggcgc gagaatggtt
     7141 cgcacggagg cgaagcagta ctgtgaaagg agaaagtcct taagatggaa ggatttttca
     7201 gcgtctttac tgtagaactg ctaaacagcg gaatccggct ggcaacgccg atcttgttag
     7261 ccgcgctggg tggggcgatc tgcaaccagg ctggcgtgct taacctcgct ttggaagcca
     7321 aaatgctgct tggggccttt gtgggaattg tggccgccta ttatctgggc aactcctatg
     7381 gcggattgct ggtcgccatg atcgcaggtg gcctgcttgg attgctcttc gcgtttctct
     7441 atcataaata tagagtcgac cttgtgattc ttgccattgc gtttaatcta atcgtcgtcg
     7501 agctgactgt gttcgtaatg cgcgccatgt tcggtaacgt gggcagttgg tctgatccca
     7561 gcatcgagcg cattcccgat atacagattc cattacttaa ggacatccca ttcctgggac
     7621 cgctgctcag cggatacaac atcatcgttt atttttcgtg gctggttgtc atcgtgatgt
     7681 ttgtctatct ctatcgaatg cgaggcgggc gacatctacg cgctgtgggc gaaaataaaa
     7741 gtgcggcgga gacagtaggc atcaatgcca ttctgctgca aactcttgcg ttggtgatca
     7801 gcggagcgct ggcagcgtta ggcggtgctt tcctttccat tggtcatcta aaattgttca
     7861 cgcgcaacat gagcaacgga cgtggctgga tcgcgattgc cgcagcgatg tttggcgtga
     7921 accatcccat cggcgttttt ctggccagct tgttctttgg ctttgctgat gcattttccg
     7981 tccgcatcca gaacgtaacg gatctgccgc cgaacctcgt aaagctctta cccaatgttg
     8041 caacattact cgtgttaatc atcatcgcgc tgcgcggcat gattgccgta ggcgtggcga
     8101 gacgtcgttt tcgtgcccag ctgcgtgctg aggaaactgc tgcacctgta caatcatctt
     8161 cttagagaaa tatcttgata ttcagaccct aaggttttcc tggcgaaacc acacaaagtc
     8221 tgtgagcaat caccgcgtgg gctttgtatt gtattgcaat cgaaacctta gggtttcttt
     8281 taatatcgtg cattatgacc aaa
//
LOCUS       DASTJP010000193         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_564821, whole genome shotgun sequence.
ACCESSION   DASTJP010000193 DASTJP010000000
VERSION     DASTJP010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564821"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VFQ23_05895"
     CDS             <1..1128
                     /locus_tag="VFQ23_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906148.1"
                     /translation="QGPTPIRVVTALLILVIGIYAASTFEEIINGLVLWTLTIILALH
                     VSGVSERLAQSLARDDTFTGRRFWNLSQSFLALSVFVILAGVNIRMLSDALRNGPLWF
                     NDYGLGGMQYGAFQIFDIIEEYLSEHPDTKIIFSPDWANGADTVARYFLDDYSSIQMG
                     SIQGHVIQKLPLDSTTLFIMTPYEYADIKDNPKFTDVDVETIVPYPDGSPGFYFVRLR
                     YVDNVEEVFAAEKAVREELRESTLLIDGQQVKLRYSYLDADFQDKSMALVFDNDPFTV
                     AKTFETNPFVIEMTFPKARMLNGFSIIIGSASVQITLKCYATTDAEPITYTFEGQGTN
                     QQPELSFDFSKPTKAQVLRVEVLDPHAHDQAKVHIWELKLR"
     gene            1177..>2585
                     /locus_tag="VFQ23_05900"
     CDS             1177..>2585
                     /locus_tag="VFQ23_05900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9906149.1"
                     /translation="MSLPRLFIKAVAPIASILLLLAGIMQLRAQVWTFGAICIVLAMF
                     GFILSMRLLEKAPFTTEELETLRPWAIPAILWITVISLLMISVFYVADNFKTAQTDRI
                     AAIAWIVSLILGIFAIWGKPSRAGNHPTLAEKLKANRTEVFFLVVVILLAFAMRTIDL
                     VSHPYPWSGDEASIGTEATRILNGEITNFFDTGWSSQPNWSFLPTAISEMIFGRNILA
                     VRIPSALAGTLAVLFVYLAAREFFNPIVALMAAAFLATLPYNVHFSRIGVNNVVDSLM
                     SALIFWLIARAMKTDDPRYYFSAGVVGGLCIYTYAGTRLALILGGLAILFLIVRQKGY
                     LFSHWKHLIAFSAGIVISAAPQAAHFARHPDIFIGRLGQEGILFNGWLTQQAALTGKS
                     VWDILWSQFTRTTMVFIASAAPGNFFNSPVPYLTIFGSVLFLLGMGYALAFGLEPSHF
                     IILLWFWAVILFGGILTLNP"
BASE COUNT          569 a          655 c          659 g          702 t
ORIGIN      
        1 cagggcccta cccccattcg agttgttacc gccctgctga tcctggtgat tggcatctat
       61 gccgcttcga cgtttgaaga aatcattaac gggctggtgc tctggacgct cacgatcatc
      121 cttgccctgc acgtgtctgg agtttcggag cggctggcgc aatctctcgc acgcgacgat
      181 acttttacag ggcgaaggtt ttggaatctt tcacagtcct tcctggcgct atccgtgttc
      241 gtcatcctgg cgggcgtgaa tatccgtatg ttgagtgatg ccctccggaa tggaccgctt
      301 tggttcaatg actatggact gggtgggatg caatacgggg cgtttcagat attcgatatc
      361 attgaagaat atctgagcga gcacccggac acaaagatta tcttctcgcc ggattgggca
      421 aacggtgcag acactgtggc gcggtatttt cttgacgatt attcatccat ccagatgggc
      481 agtattcaag gacatgtcat acaaaagctg cctctggaca gtactacctt gtttatcatg
      541 acaccatacg aatacgccga catcaaggat aacccaaaat ttacggatgt ggatgtggag
      601 acgattgtcc cttatccaga tggcagcccg ggtttctatt tcgtccgcct gcgttatgtc
      661 gataatgttg aagaagtatt tgccgcggaa aaggcagtcc gtgaagaatt gcgagaatct
      721 actcttctga ttgatggaca gcaggtcaag cttcgctact cctatctgga tgccgatttt
      781 caagacaaat cgatggctct ggtattcgac aatgatcctt tcaccgtggc aaaaacgttc
      841 gaaacgaatc catttgtgat tgaaatgacc tttcccaaag cccgcatgct caatggattt
      901 tcgatcatca ttggttctgc aagtgtccag atcacgctga agtgctatgc cacgaccgac
      961 gccgagccca taacgtatac attcgagggg caaggaacaa atcaacagcc ggaactttcg
     1021 tttgattttt ccaaaccgac aaaagcacag gttctgcgag tggaagtgct ggacccccac
     1081 gcacatgatc aggcaaaagt ccatatttgg gaattgaagc ttcgctgatc aaagtgagtt
     1141 cgacttgaaa caagaccagt tttcatcagg tggttcatga gcttgccaag attattcatc
     1201 aaagcggtgg cacccattgc atccattttg cttctcctgg cggggatcat gcagctgcga
     1261 gcgcaggtat ggaccttcgg ggcgatctgt atcgtgcttg ccatgttcgg gttcatcctg
     1321 agcatgcgtt tgttggagaa agctcccttc acaaccgaag agctcgaaac gttgcgcccc
     1381 tgggcaattc ccgcgatcct ctggataacc gtcatcagcc ttttgatgat ctcggtcttt
     1441 tatgttgccg ataatttcaa aactgcccaa acggatagga ttgcagccat agcgtggata
     1501 gtcagtttga tcctggggat ttttgcgatc tgggggaagc catcccgggc aggaaatcat
     1561 ccaactctcg cggaaaaact caaagcaaat cgaacagaag tattcttttt agtggttgtc
     1621 atccttctgg catttgccat gcgcactatt gatctggtaa gtcatcctta tccatggtcg
     1681 ggtgacgagg cttccattgg cacggaggcg acgcgcattc tcaacggcga gatcaccaac
     1741 ttctttgata cgggctggtc cagtcagcca aactggtcat ttctgccaac cgcgatcagt
     1801 gagatgatct ttggaaggaa tattttggcg gtccggatac ccagtgcgct ggcgggcaca
     1861 ctggcggtcc tttttgtata cctggcagcc cgggaatttt tcaacccgat cgttgctttg
     1921 atggcagcag ccttcctggc aacacttccc tataacgttc atttcagccg cataggggtt
     1981 aacaatgtag tcgacagttt gatgtctgcc ctcatcttct ggctgattgc ccgtgcgatg
     2041 aaaacagacg atccgcgcta ttactttagc gcgggtgtgg ttggcggttt gtgcatttac
     2101 acctatgccg gtacacggtt ggctttaatc ctgggcggac tcgccatcct gtttttaatc
     2161 gttcgtcaaa aaggctatct tttttcccac tggaaacatc tgatcgcatt ctctgccggg
     2221 atcgttatca gtgcggcacc acaggccgcg cactttgctc gccacccgga tatctttatc
     2281 ggtcgcctcg ggcaggaagg gatcctcttc aacggctggc tgacccagca ggctgcactg
     2341 acgggaaaaa gtgtctggga tattctctgg agtcagttta cccgaaccac catggtgttc
     2401 atcgcctcgg cagcgccggg gaatttcttc aactcccccg ttccctattt gacgatcttt
     2461 ggcagtgtgc tcttcctgct gggaatgggg tatgcactgg ctttcgggtt ggagccaagt
     2521 cattttatta tcctgctctg gttctgggcg gtcatcctgt tcggcggcat cctgacgttg
     2581 aatcc
//
LOCUS       DASTJP010000194         4170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1183480, whole genome shotgun sequence.
ACCESSION   DASTJP010000194 DASTJP010000000
VERSION     DASTJP010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183480"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFQ23_05905"
     CDS             complement(<1..620)
                     /locus_tag="VFQ23_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9906150.1"
                     /translation="MKHHSRFSPHQRPPWWPENEEWPPKRWRYMRGRPFFRRMGCFFV
                     IFIFFAFTGFLALLRFLLAPVIDFHGVPPLDRPDLIFPFGLAGFLILLAAFFWSGRAL
                     RRLSMPLDDLLDASNKVAEGDYSVRVEAKGTPEIYSLMQGFNSMVERLQANDLHRRNM
                     LADVSHELRTPITVIQGNVEGILDGLYPADEERLKSIMEETQILSRL"
     gene            complement(762..1448)
                     /locus_tag="VFQ23_05910"
     CDS             complement(762..1448)
                     /locus_tag="VFQ23_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906151.1"
                     /translation="MKSILVVDDEPKITQLVRDYLERAGFGVLVAHDGRKGLSLAKTE
                     KPDMVILDLGLPQLDGLDFTREFRKTSNAPIIMLTARAEETDKLIGLELGADDYMTKP
                     FSPKELVARVRVVFRRIENATSAGSEMIRVADLTLDVPRLRVTAERREIEELTPTEFE
                     LLASLARQPGRVFTRAQLLDAIHGVAFESYERAIDAHIKNIRRKIEVKASEPKYVLTI
                     YGVGYKFADR"
     gene            complement(1522..1932)
                     /locus_tag="VFQ23_05915"
     CDS             complement(1522..1932)
                     /locus_tag="VFQ23_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906152.1"
                     /translation="MNRNIGLRILSGLVLIAAVAGIAFFAFQAGIAQGSPITVESPSG
                     ETVPAPYPYYGYGMPFHYRPWGFGFGCFGPLLAFFLLFVAFRFLFWGPRWGWRHHYGG
                     HGPWRHDAGERGVPPFFEEWHKRAHGETPEEKKE"
     gene            2094..2606
                     /locus_tag="VFQ23_05920"
     CDS             2094..2606
                     /locus_tag="VFQ23_05920"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9906153.1"
                     /translation="MNIRYGTTADATMLSELGAKTFYDTFAKDNTPENIGTYLKRSFS
                     PELQFDELSSPDNIFLIVESEEVPIGYAQLILNSKDDAIQRARPLEIRRIYALQEYLG
                     KGVGKELMRATINEARQRGCDSVWLGVWEKNQRAIDFYKKWGFREVGTHLFSVGDDPQ
                     NDFIMELGLN"
     gene            2728..3279
                     /locus_tag="VFQ23_05925"
     CDS             2728..3279
                     /locus_tag="VFQ23_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906154.1"
                     /translation="MVEILKKHKVEKMIELTQNSFVKRNSRLPELMVENMIKVITRSS
                     LISVFEKPKLRDFARSLSNEEKKKLAKGLENLFHGDEQLGFETMLEVLKNGKLAKWSL
                     MTICQTYYRPQVEVFVKPTTVKGIIDYFEIKNLQYKPTPTWAFYKEYRELINEMKTKV
                     DPSLSPYNVAFSGFLMRSMQGLQ"
     gene            3294..3944
                     /locus_tag="VFQ23_05930"
     CDS             3294..3944
                     /locus_tag="VFQ23_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906155.1"
                     /translation="MINLSHIRIAVCAAILFTLLAHPSSTSACSCGAGVTVTEEFAQD
                     SAVFTGKVIRIVDNYFPYFFTADNILYKIGRPTYFFDQFLRNDDKRLNLSIFFKVLNS
                     WKGVEKTLVEVNTGRGDSDCGYSFVNGQEYLIYANFAYGIPDNYWVTSICTRNAMLHD
                     ASEDLKYLNSLPELPLKFALPIPWAETDSITVVIIVIIGVIVSVMRGLRMQREREV"
BASE COUNT         1215 a          967 c          978 g         1010 t
ORIGIN      
        1 agccgcgaaa ggatctgcgt ctcctccata atggacttca gccgctcttc atccgcagga
       61 tacaaaccat cgagaatccc ctctacattg ccctgaataa cggtaatggg tgtgcgcaat
      121 tcatgcgaca catctgcgag catgttccgc ctgtgcaagt catttgcctg caagcgttct
      181 accatcgaat taaaaccctg catcagggaa taaatttccg gcgtaccctt cgcctcgacg
      241 cgtacgctgt aatctccctc tgccactttg ttcgaagcgt ctaatagatc atccagcggc
      301 atcgacaggc gacgcaaggc gcgcccactc caaaagaagg cagccaacaa aatcaggaac
      361 ccggcaagac caaacggaaa aatgagatcg ggtcgatcca gtggcggcac accgtggaag
      421 tcaatgaccg gagccaatag gaatcggagc aaggccaaaa agcctgtgaa agcgaaaaaa
      481 ataaagatga caaagaaaca gcccatccgg cgaaagaacg gcctgccgcg catatagcgc
      541 cagcgtttgg gcggccattc ctcattttca ggccaccacg gcgggcgttg atgaggggaa
      601 aaacgagagt ggtgtttcat aatcagagta aatcggtggt tgagtagccc tgagcgagct
      661 ttgcgaaggg catatcgaaa ccaccaattt caatagaaat ttcacgtgca acgggtggtc
      721 tcgatacgga cttcgtccta ctcgaccacc aataaataaa gttatctatc cgcaaactta
      781 tacccaaccc catagatggt caaaacatat tttggctcac tggctttgac ctcgatctta
      841 cgacgaatat tcttgatgtg ggcatcgatc gcgcgttcat aggattcaaa ggcaacgccg
      901 tggattgcgt ctaataactg agcacgggta aaaacgcgac cgggttgacg cgcaagcgag
      961 gcaagcaatt caaactcggt aggagttaat tcttcgatct cgcgtcgctc cgccgtaacc
     1021 cgcaacctgg gaacatcaag agtgagatct gcgactctga tcatttctga gccggcactg
     1081 gtcgcgtttt cgatgcgccg gaagacgacg cgcacacgag caaccaattc tttcgggcta
     1141 aagggcttgg tcatgtaatc gtctgcgccg agttcgagtc cgatgagttt atccgtctct
     1201 tcggcgcggg ctgtcagcat gatgatcggc gcgttggagg ttttgcgaaa ctcacgggta
     1261 aagtccagcc cgtcgagctg aggcaggccg aggtccagaa tgaccatatc aggtttctca
     1321 gtcttcgcaa gagatagccc ttttctgcca tcatgcgcga ctaaaacacc aaaccccgcg
     1381 cgctccagat aatcgcgcac gagttgggtg atctttggtt catcatcaac gacgagaatg
     1441 gatttcataa gcttttgtag gtcggacact tgcaccgcac gcaagtgcag gtgttgacaa
     1501 tccgacctac attatacgtt cttattcttt cttttcttcg ggcgtctcgc catgggctcg
     1561 cttgtgccac tcttcaaaaa atggagggac gccacgttca cccgcatcat gccgccaagg
     1621 accatgtcca ccataatggt gtcgccagcc ccaacgcggt ccccagaaga ggaagcggaa
     1681 agctacgaat aagaggaaaa aggcaagcaa cgggccgaaa caaccgaagc caaatcccca
     1741 tggacgataa tggaatggca ttccatatcc ataatatgga tacggcgcgg ggaccgtttc
     1801 acccgatggc gactcgaccg taatgggcga gccctgtgca atgcctgcct gaaaggcaaa
     1861 gaaggcaatg cctgcaacag cagcaatcaa caccagccca gacaggatac gtaaaccgat
     1921 atttctgttc attttattct cctttatttg aacttatgca tggagtatag aatccgcttg
     1981 tgaattgatt gtgaagagaa gagggagaag accggttttc gtttacgctt catgaacgcc
     2041 cgtacgctgg cagctgttaa aatcagccaa ggactgtcta tcaaggagca aagatgaata
     2101 tccgttatgg aacgacagca gatgcaacga tgctatctga gctgggtgca aagacttttt
     2161 acgatacatt cgcaaaagat aacacgccag aaaacatagg cacttatttg aaaagatcat
     2221 tttcgccgga gctccaattc gatgagcttt ccagtccaga caacattttc ttgatcgtgg
     2281 aatctgagga agtacctatc gggtacgctc aactcattct aaacagcaaa gacgacgcca
     2341 tccaaagggc ccgtccgctc gagatcagaa ggatttatgc attacaagaa tatttgggta
     2401 agggagtggg gaaagaactg atgcgcgcaa ccatcaatga agcccggcaa cgaggctgtg
     2461 acagcgtctg gctgggcgtc tgggaaaaga atcaaagagc gattgatttc tataaaaagt
     2521 ggggatttcg cgaggtgggt acacatctat tttcagttgg agatgatcca caaaatgatt
     2581 tcatcatgga gttgggattg aattagctct tcaagattga cgtgacaaac tacatcttct
     2641 acaatccaaa atatgaatct caataagcta aaacaagccg aagcagcatt ttttgatcgg
     2701 tatccgggag gcttcgcgga ccctgagatg gttgaaattc tcaaaaaaca caaagtggaa
     2761 aagatgatcg aacttactca gaatagcttc gtaaaaagaa actccaggct ccccgaattg
     2821 atggtcgaaa acatgatcaa ggtcatcacg cgttcatccc tgatttctgt ttttgaaaag
     2881 cccaaactca gggattttgc tcgttcactc tcaaatgagg agaagaagaa actcgcaaaa
     2941 ggactcgaaa acctatttca tggagacgaa caacttggat ttgaaactat gctagaagtt
     3001 ctaaaaaatg gaaagctggc aaagtggtct ctgatgacga tatgtcagac ttattatcgg
     3061 ccgcaggttg aggtcttcgt aaagccgacc actgtaaaag gaattattga ctactttgaa
     3121 ataaaaaatt tgcaatataa gccgacacca acgtgggcct tttacaagga gtaccgagag
     3181 ctaatcaacg aaatgaaaac taaggtagat ccctctcttt ccccctacaa tgtggcattt
     3241 tcgggatttc ttatgcgaag tatgcaagga ttgcaatgaa cagtaggtgc agaatgataa
     3301 acttaagtca catccgcata gcagtttgcg ccgctatctt atttacatta cttgctcacc
     3361 cttcatccac aagtgcctgt tcatgtggcg caggtgtgac tgttacggaa gagttcgcgc
     3421 aagatagtgc tgtatttaca gggaaggtta tcaggatcgt tgataattat ttcccctatt
     3481 tttttacagc agacaacatc ctgtataaga tagggcgtcc tacctacttc tttgatcagt
     3541 ttcttcgtaa tgatgataaa agattgaact taagcatatt tttcaaggtc cttaattcgt
     3601 ggaaaggggt cgaaaagaca cttgtagaag tgaatacggg acgcggggac agtgattgtg
     3661 gatattcttt cgtaaatggt caagaatatt tgatttatgc aaattttgct tatggaatac
     3721 ctgataatta ttgggtaaca agcatctgca ccagaaatgc tatgttgcat gatgcatcag
     3781 aggacctgaa atatctgaat tctctacccg aattaccatt gaaatttgcc ttacctattc
     3841 catgggcgga aacggattcc attacagtag tgatcatagt aattattggc gtgattgtat
     3901 ctgtgatgcg cggtcttcga atgcagcggg aacgagaagt ttgacctgat tattggaaaa
     3961 tactatgaat aaatccatgc caaacctaaa catttataaa caacggagta tacacaatga
     4021 agaatccaag agcacggtta atagcagtct ttctaaatgg ggtctttata cttgcttgtt
     4081 actctacaac cctgcctagt ttcgcgacgt caacgcctca aaacatcatc actgtaccac
     4141 cgatgccaac gacgttctta actcaaccgc
//
LOCUS       DASTJP010000195         5636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_883501, whole genome shotgun sequence.
ACCESSION   DASTJP010000195 DASTJP010000000
VERSION     DASTJP010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883501"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..514)
                     /locus_tag="VFQ23_05935"
     CDS             complement(23..514)
                     /locus_tag="VFQ23_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906156.1"
                     /translation="MQTLNSKPLDDYLKWREVVFSVTGSQVAEITDQPQQVYGVIMDV
                     GLSNDFIISVTAFASGEASLRTTVGGGAIGLGSDPFIAEQAEQIITRAQSLALSARQI
                     HNYNLPTQKEVYFYFLTPAGLKLTKATMEEVGSRAHPFHEMFSRFSAIKARSEELLRS
                     NKP"
     gene            complement(639..989)
                     /locus_tag="VFQ23_05940"
     CDS             complement(639..989)
                     /locus_tag="VFQ23_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906157.1"
                     /translation="MDVKIKPLSGCLQIVLGVLTLGIAPLAAWLNERAWPKSVDEQGL
                     TTRAGTNIAWGDFTKIKKVITKIGQTSTTTEHYELSGPNGKVVVAPYRLENGNQVFDF
                     IWRRLPERIKQPQN"
     gene            complement(1094..2491)
                     /locus_tag="VFQ23_05945"
     CDS             complement(1094..2491)
                     /locus_tag="VFQ23_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906158.1"
                     /translation="MKTKSVTLLLGILIALMTFACIGGGAATPTSEPADVADLSYTAT
                     SEPVIVEEGEVKILNSTSYLDRYNDYYVVGELLNDSNQMIENIVISLSIMDESGASLL
                     KDDNDNPVDSIDIYPYIDVLSPGTSAPFSYYISIADLQPATYEVTVRSYDRSSASELK
                     EFDVQNVQTHFMENGDVVLAGEIVNLDSNHVDVESLAGTVLDSTGTVLAANSTMTYSR
                     YLYPAGDVEGRDRGPFVITLYGPIENVSQWKIFARSIENTTVPAADLDIELAGSYVDG
                     SGTYHLLGTVTNNGSSQVSPSVVGGLYGEDTLVWDAATSNIPLYLNSGDSAPFDLSTF
                     QVVPYMSPDQMSAVDEIVVPDLYWSYSTDYQVVSLAAEQVELMQGSYDWTLNGSVVNS
                     SGENLSRIIALVQFLDENGQVMATNSTTMFPAEGSDAIEPGVSTEFSISIYASEDWNL
                     SSQAYQVILQGVIAE"
     gene            complement(2592..3437)
                     /locus_tag="VFQ23_05950"
     CDS             complement(2592..3437)
                     /locus_tag="VFQ23_05950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906159.1"
                     /translation="MWIQNLELSEQKQNVARVYDLAAAGYDKPALRFFSLTSERLVQF
                     AGIRAGDHVLDAGTGTGLAAFAAAERVGAAGKVIGVDIGEEILQQASRKLEADSHNSI
                     SFSLGDMEHLEFSDQSFDAVLSAASLFFLKDMAAGLQEWKRVLKAGGRIAVSGYGDSA
                     FRPLSDLFEARIRSYGVQLAAPIRPFSWQRLTDPEQYFSLFRIVGLENIEVYSEQLGY
                     YLTSASEWWDVVWNSGFRGPISQLTPEQVSQFKQEHLAEVQNLATSKGIWLDIAAIFA
                     VGQKG"
     gene            complement(3591..>5636)
                     /locus_tag="VFQ23_05955"
     CDS             complement(3591..>5636)
                     /locus_tag="VFQ23_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906160.1"
                     /translation="WWIGLASIQDEKLLAPTIKKVLGIPDSQTTSAEESILQFLQSRQ
                     ALLVLDNCEHIISSCAHLVQNILVNCPRVKVLATSREALSIPGEIAWLVPSLSLPPLN
                     QMQDLLSWECPRLFWERATSYRSDLQMTETNSQALLRICHALEGIPLAVELAASRVKT
                     LSLEQLASRLDDKLNLLTTGSRTSQPRQQTLRATIDWSYDLLSEPEQIAFRRLSVLAG
                     SWTLDSTSSVTSWNGTTSDHALDMLTRLIDKSLLVVDADDGEVRYRMLEILRQYAFEK
                     LQLADEVETVRRQHLSYYALWAQNMKPGWYNRQQANRIKQFDAEYPNLRLALAQGLDE
                     RADENDLLPGVQLATALGPFWSFNGEYNEGQMWLQKAIHRLNTVAAGVNDESKHSKEL
                     LSLKAKALYEYGFLVWFQSNYDQARIIFLECSELYEGLQDLSGLAYSNIYLAHSTWGL
                     GERDTAWQMWTESLEQFKRTNDFWGAGLVHSFRGRATRADGQYEQAEWEYKQGIHYYD
                     TVGDDWGKGICLSHLGMLAFQKNDPQTAIGLFEERLRTAQKLGFRQSIAYSNFLIGMA
                     AWKLGKPEQVRRYMREALAYMYDIQNFATMANCLLGFAWADSEEGNLTQAAYLMGVVT
                     RADEIPTLKMEFEDVYFHLPILAHLQSKLQDASYQAAFEKGRNTPLDQAAKKILETY"
BASE COUNT         1358 a         1483 c         1407 g         1388 t
ORIGIN      
        1 gcaacttaca ctgagtatta cctcaaggtt tattgcttct caaaagctcc tccgatcgag
       61 ctttgatggc agaaaacctc gaaaacatct cgtgaaaggg gtgggctcga gagccgactt
      121 cttccatagt agctttagtt aactttaaac ctgctggagt aagaaagtaa aaataaactt
      181 ctttttgtgt cggcagattg tagttatgaa tctgcctggc tgacagcgcg agggattgcg
      241 ctcgggttat gatctgttct gcctgttctg cgatgaatgg atctgaaccc agtccgatcg
      301 cgccacctcc aaccgttgtc cgcaaggaag cttcacctga cgcgaatgct gtcacactaa
      361 tgatgaaatc gtttgaaagc cccacatcca taattacgcc ataaacttgt tgtggctgat
      421 ccgttatctc tgctacctga gagccggtga ccgaaaaaac cacttctcgc cattttagat
      481 aatcgtccaa cggctttgaa tttaatgttt gcatttcatg ttcctttcgc aatagtccct
      541 gatgggaata cctggtaatt cattgtctat ttggcggaag gacagcacgc catccttccg
      601 ccaacgtcac ctagcaactg aagctttggc caggctgatt agttctgagg ctgtttgatc
      661 cgttccggga gacgtcgcca aataaagtca aagacctggt tgccattctc cagacggtac
      721 ggcgccacca caacctttcc gttaggtcca ctcagttcat aatgttcggt cgtggtgctg
      781 gtctgaccga tttttgtaat gaccttctta attttagtaa agtcacccca tgcgatgttg
      841 gttccagcac gcgttgtgag gccctgttca tccacagatt tgggccaggc acgctcattc
      901 agccatgccg cgagcggagc gatacccagg gttaacaccc ccagaacgat ttgaagacag
      961 ccagacaatg gtttgatttt gacgtccatg atattttctc cttaatatag tgattgaaat
     1021 gtttggtttt tgaattgcag ccagacagac attaagtacg gtcatgtatg cgccacggta
     1081 ctaagagtga cttctattca gcaatgacac cctgcagaat gacctggtaa gcctgcgatg
     1141 agagattcca gtcctccgaa gcatagattg aaatgctgaa ctccgtcgaa acgccgggct
     1201 cgatggcatc cgaaccttct gccggaaaca tggttgtcga attggttgcc atcacttgcc
     1261 cgttctcatc caggaattgc accaaagcga tgatgcgact caggttctcg ccggaactgt
     1321 tgaccacaga accgttcaag gtccagtcat acgaaccttg catgagttcc acttgttctg
     1381 ctgcgagaga tacgacctgg tagtcggtgg agtagctcca atacaggtcc ggtacgacga
     1441 tctcgtccac tgccgacatt tgatcaggcg acatatacgg aacgacttga aaggtgctga
     1501 ggtcgaaggg tgctgaatct ccggagttca agtagagcgg aatattcgag gtcgcggcgt
     1561 cccagacgag cgtatcctca ccatacagac cgccaacaac ggaaggggag acctggctgg
     1621 aaccgttatt cgtcacagtg ccaagcaagt gataggttcc tgaaccgtca acataggagc
     1681 cagccaattc aatgtccagg tcggcagccg gaacagtcgt gttttcaatg gagcgtgcga
     1741 aaatcttcca ctgcgataca ttctcaatcg gaccatacag tgtgatcaca aaaggaccgc
     1801 ggtcgcgtcc ttcaacgtcc ccggctgggt aaagataacg ggaataggtc atggtggaat
     1861 tcgcagcgag caccgttccg gttgaatcca gcacggtacc tgccaggctt tccacatcaa
     1921 cgtggttgga gtccaggttc acaatctcgc ccgccagaac gacatcgcca ttttccataa
     1981 agtgcgtctg cacattctga acatcaaatt ccttgagctc agatgcggag gagcggtcgt
     2041 aactcctgac cgtgacctca taagtggccg gttgtagatc agcgatagag atgtagtagc
     2101 taaacggcgc ggatgtgccg ggagacagga catcgatata gggatagata tcaatgctat
     2161 cgaccgggtt gtcgttgtca tctttgagca gactggcacc agactcatcc atgattgaga
     2221 gcgagatgac gatgttttca atcatttgat tcgaatcgtt gaggagctct cccacgacgt
     2281 agtagtcgtt gtaacggtca agataactgg tcgagttgag gatcttaacc tcgccttctt
     2341 ccacgatcac gggctcgctc gttgccgtgt atgaaaggtc agcaacatcg gccggttcac
     2401 tggttggcgt ggcggccccg ccgccgatgc aggcaaaggt catcaatgcg atgaggatgc
     2461 cgagcaacaa agtaactgat ttggttttca tgatttattc tccttttggt tatggaattt
     2521 ttttcagttc attgatttct gcgatttctg cacagaaata atgactggtt tacaaaaatc
     2581 gacagtgcct attacccttt ttggccgacc gcaaaaatcg ccgcgatatc gagccagatg
     2641 cctttggatg ttgccaggtt ttgtacttct gccaggtgct cctgtttgaa ttgagacact
     2701 tgttctggcg tcagttgcga gatcgggccg cgaaagccgc tgttccagac aacatcccac
     2761 cactcgctgg ctgatgtcag atagtagcca agctgttcgg agtagacttc gatgttttcc
     2821 agcccgacaa ttcgaaacag cgagaagtat tgttcgggat ccgtcagccg ctgccacgaa
     2881 aacggacgga tgggtgccgc aagctggact ccatacgacc gaatgcgcgc ctcaaacaga
     2941 tcggagaggg ggcggaaggc cgagtcgccg tagcctgaaa cagcgatccg tccaccagct
     3001 ttgagcacac gcttccattc ctgcaggcca gcagccatgt ccttcaggaa gaagagtgag
     3061 gcggcgctca acacggcatc aaaggattgg tctgagaact caaggtgttc catgtcaccc
     3121 agagaaaatg aaatcgagtt gtgcgaatca gcctccaatt ttcgggaggc ttgctgcagg
     3181 atctcctcac cgatatctac gccgattact ttgcccgccg caccgactct ctccgcagcg
     3241 gcgaaagcag ccaaccccgt gccggtgcct gcgtccaaca cgtgatcacc tgcgcgaatg
     3301 cctgcaaatt gcaccagcct ctctgaggtc agggaaaaga agcgcagcgc gggtttgtcg
     3361 tagcctgcgg cggccaggtc atacactctg gcaacgttct gtttttgttc actcagttca
     3421 agattttgta tccacatagg gctctccttc tgtaatgttg gtgccatgat acaaaccccg
     3481 cgccaaatta ccgccaaatt tttcaaccag attcgccaaa tttggcaccg atttaaagag
     3541 aaaaccgcca tcaggcggtt ttcttgaaga attcagcgaa caacgtagtg ttagtaggtt
     3601 tccaggatct tttttgcagc ttgatccaac ggagtgttcc gtcctttttc aaacgccgcc
     3661 tgatacgaag catcctgcaa ctttgactgc aagtgggcaa gaatcggcag atgaaaatag
     3721 acatcttcaa attccatttt gagcgtcgga atttcatctg ccctggtgac aacacccatc
     3781 aaataggcgg cctgtgtgag attcccttct tctgagtctg cccatgcaaa cccaagcagg
     3841 caattggcca tggtggcgaa attctgaata tcatacatgt aggccagcgc ctctcgcatg
     3901 tatcttcgta cctgctcagg cttgcctaac ttccaggctg ccatcccgat cagaaaattg
     3961 gaataggcga tcgactgacg aaagcccaat ttctgcgcgg tccgcaaccg ctcctcgaat
     4021 aagccgatgg cggtttgcgg atcattcttt tggaacgcca gcatgcccaa atgactcaag
     4081 caaattccct tgccccaatc atcgcctact gtatcataat agtggatacc ttgtttgtat
     4141 tcccactcgg cctgctcata ctgtccatcc gcgcgcgttg cccggcctcg aaagctatga
     4201 accaggccag caccccagaa gtcattggtc cgcttgaact gctccaagga ttctgtccac
     4261 atctgccagg ctgtatctcg ttcccccaaa ccccaggtcg agtgcgccag ataaatattt
     4321 gaatatgcca gccctgagag gtcttgaagt ccctcataga gctcagaaca ttccaggaaa
     4381 atgatccgcg cttgatcgta attggattgg aaccagacca ggaaaccata ttcatacaat
     4441 gcctttgctt tcaggctcaa gagttctttg ctatgcttcg actcatcatt gacacccgcc
     4501 gcaactgtgt tcagcctgtg gatggctttt tgtagccaca tttggccttc attgtattct
     4561 ccgttgaaac tccagaacgg acccaatgca gtagccagtt gtacaccagg cagcagatca
     4621 ttctcgtccg cacgttcatc cagtccttgt gcgagtgcca ggcgaagatt tggatattcc
     4681 gcatcaaatt gtttgatacg gtttgcctgc tgacggttgt accagccggg cttcatgttc
     4741 tgcgcccaca gtgcgtaata agataaatgc tgacgcctga ccgtttccac ctcatcggcc
     4801 agctgcagtt tctcaaacgc atactggcgg aggatctcca gcatgcgata gcgcacctca
     4861 ccgtcatcag catccaccac cagcagagac ttatcgatga gacgcgtcag catatccagg
     4921 gcgtggtcag aggtggtgcc attccacgag gtcacgctgc tggtcgaatc gagcgtccaa
     4981 cttccggcca gaaccgagag tcgccgaaac gcgatctgct ctggctcgga gagcaaatcg
     5041 tagctccaat cgatcgtcgc gcgtaaggtc tgttggcgag gttgagacgt gcgactgccc
     5101 gtggtcaaca aattgagttt gtcgtcgagt cggctggcaa gctgttcgag cgaaagagtc
     5161 tttacccggg atgcggccaa ctccaccgcc agcggaatgc cttccaatgc gtgacagatc
     5221 cgtagaaggg cctgcgaatt cgtttctgtc atctgtagat cagaacggta agaagttgct
     5281 cgttcccaga acagacgcgg acattcccaa cttaataggt cttgcatttg attcaacggc
     5341 ggcagcgaca aagaggggac tagccaggcg atctcgccag ggatactcag ggcttcgcgg
     5401 cttgtcgcaa ggaccttgac tctcggacaa ttcactaaga tattctggac caggtgagcg
     5461 caggaggaaa tgatgtgctc acaattatcg agcaccagta acgcctgccg tgattgtaaa
     5521 aactggagga tggattcttc cgcggaggtt gtctggctat ctggaatgcc aagaaccttt
     5581 tttatggtcg gcgctagcag cttttcatcc tggatggagg caagcccgat ccacca
//
LOCUS       DASTJP010000196         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_219965, whole genome shotgun sequence.
ACCESSION   DASTJP010000196 DASTJP010000000
VERSION     DASTJP010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219965"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFQ23_05960"
     CDS             complement(<1..497)
                     /locus_tag="VFQ23_05960"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9906161.1"
                     /translation="MADLRTTVILKTDIVDSTPRVAALTQSEMGLQKRQHKLFISEIV
                     LKHRGVIFQEEGDAYWIEFPAVTTAVLAAIEMHQNLRSMQAGKGDKQRLAIRVVITVG
                     DILHQETDTIGPAMSLTARIEKVTPPDEIYLSQAAWLVLNKAEVQTSFVNEFHLKGFT
                     ELEKV"
     gene            complement(541..1374)
                     /locus_tag="VFQ23_05965"
     CDS             complement(541..1374)
                     /locus_tag="VFQ23_05965"
                     /inference="COORDINATES: protein motif:HMM:NF022431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET9906162.1"
                     /translation="MDKEFWADLSKNDYQIPEGHALEELTKTLFSYLGSTDPDSRDEI
                     AYVVYANWLKRDMFSLGEIENHINELLANLEKGVGETESDSVFLRAFSVLLLAEIVHN
                     DNKKSLLEKEQIQRILERGLWYLEAENDPRGYIPVKGWAHALAHTADLMLVLGRNRYL
                     EGNDLTRILQGIANKIKQQNNYVYIHREDERLANAVAEILQRGVLDLEYVESWAGGFL
                     QPEKFTFDGTFKEDISRLYQNTRNLLRSVHIQVFSRAEIRNKENLQMLLLKALQSLQQ
                     F"
     gene            complement(1536..>2059)
                     /locus_tag="VFQ23_05970"
     CDS             complement(1536..>2059)
                     /locus_tag="VFQ23_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9906163.1"
                     /translation="FIACAEHLIAAGYTRKEKLSIMGRSAGGLLTGACLTMRPDLFGA
                     VIGDVPFVDVINTMSDPTIPLTTLEYDQWGNPDNKEYFDYIMSYSPYDNIRATEYPHI
                     LLTTGLNDPRVAYWEPAKFAAKLRELKTDEKLLLLKTNYDAGHAGASGRYDFLKELAF
                     EYAFLVDRLNGNS"
BASE COUNT          496 a          494 c          473 g          596 t
ORIGIN      
        1 tagacttttt cgagttctgt aaagcctttc aagtggaatt catttacaaa agatgtctgc
       61 acctccgcct tgtttagtac gagccaagca gcctgtgaca ggtaaatttc atcgggcggc
      121 gtgacttttt cgatccgtgc cgtcaggctc atcgcaggtc caatcgtatc ggtctcctgg
      181 tgaaggatgt ctcctactgt aattaccaca cggatcgcta atcgttgttt gtcacctttg
      241 cccgcctgca tggatcgtag attttggtgc atttctatcg cggcgagtac ggcggtcgtc
      301 acagcgggaa attcgatcca gtatgcatca ccctcctcct ggaaaatgac tccgcggtgt
      361 tttagcacaa tctctgagat gaatagcttg tgctgccttt tctgcaatcc catttcagac
      421 tgcgtcagcg cagccaccct tggtgtggaa tctacaatgt cagttttcaa tatgacggtc
      481 gtgcgcaggt ctgccatatt tttctaccgc tgtctcgagc ctgatcctaa tatttgtgaa
      541 tcaaaactgc tgtaaagact gaagtgcctt caataatagc atttgtaaat tctccttgtt
      601 tcgaatctcg gcgcgactga atacttggat gtgtacactt cttaaaagat ttcgcgtatt
      661 ttgatacaga cggctgatat cttccttgaa agttccgtca aaggtaaact tttctggttg
      721 caagaatccc cctgcccagc tttcaacata ctccaggtcc aatacaccgc gctgaagtat
      781 ttcggcaaca gcattcgcca ggcgttcatc ctcgcgatgg atgtaaacgt aattattttg
      841 ctgcttaatc ttatttgcga tgccctgtag gattcgcgtc aaatcatttc cttcaagata
      901 acggttcctt cccaggacca gcatcagatc ggcggtgtgg gcgagagcat gtgcccagcc
      961 tttgaccggg atatagccac gcgggtcatt ttccgcttcg agataccata atcccctttc
     1021 gagtatcctt tggatttgct ctttttcgag aagtgacttc ttattgtcgt tgtgcacaat
     1081 ttctgccaat agcagtacag agaaagctcg caggaacact gaatctgact cggtctcgcc
     1141 gacccctttt tcgagatttg ccagtaactc atttatatga ttttcgatct ctcccaggga
     1201 aaacatatcg cgcttgagcc agttagcgta gactacgtat gcaatctcat cgcgagaatc
     1261 gggatccgta cttccaagat agctgaaaag cgtcttggtc aattcctcaa gagcatgccc
     1321 ttcaggaatt tggtaatcgt ttttcgagag gtcagcccaa aattctttgt ccatttgtaa
     1381 gtaaccttaa atatgtattt caaacgacac gttaattagc taacaacgat ctgttctata
     1441 ctcttgaagc gttccaattt gtcatttctc aatgcatcgc tggagctaag aaacatactc
     1501 caatattcat tcaagagttt ggataggttg tttgatcaag agtttccatt taaccggtcg
     1561 accaggaagg cgtattcaaa cgccagttcc ttgagaaagt catatcgtcc ggaagcaccg
     1621 gcatgacccg catcgtaatt cgtcttaaga agaagcagct tctcgtcggt tttcagctcc
     1681 cggagtttgg cggcgaactt cgccggttcc caataggcaa cacgcgggtc attgagaccg
     1741 gtggtgagta agatgtgtgg atattctgtg gcgcggatat tatcgtatgg ggaataagac
     1801 ataatgtaat cgaaatactc tttgttatcc gggttacccc attgatcata ttcaagggtg
     1861 gttaagggga tggtgggatc actcatggtg ttgatcacat ctacaaaggg gacatcgccg
     1921 atcaccgcgc cgaacaggtc agggcgcatc gtcaggcagg cgcctgtcaa aagtccacct
     1981 gccgagcggc ccataatgga taacttctct ttgcgggtgt aaccggcggc tatcagatgt
     2041 tcggcacagg caatgaaat
//
LOCUS       DASTJP010000197         6216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1581176, whole genome shotgun sequence.
ACCESSION   DASTJP010000197 DASTJP010000000
VERSION     DASTJP010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581176"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VFQ23_05975"
     CDS             <1..688
                     /locus_tag="VFQ23_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906164.1"
                     /translation="TARETLRFTARFFYSGPEKRMEDRIDETLELVGLSDKADRSIKG
                     FSGGERQRLGIAQAQINYPDLLILDEPAASLDPMGRRDVLEVMERLRKHTTIFYSTHI
                     LEDVQRVSDTVAILNHGRLIAQAPIAELTSGKDGIIYSLKVKGDGKCAYQNILGHPWV
                     ESVTMDSSDSNTTLNVKVTDERRAETELLRLALTDSNTIVTQFGRRKHNLEDVFLEIV
                     EGDKSNDHHN"
     gene            672..1463
                     /locus_tag="VFQ23_05980"
     CDS             672..1463
                     /locus_tag="VFQ23_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9906165.1"
                     /translation="MTTITETLTPRTEHGWRIGLRNLLSNEMNLRWGGRRWIKSALIW
                     LAILNGFTLLVSLADEQNSTSKILAETIDVFAHVGLLATSIGAVVSTQGVIIREKQLG
                     TAAWVLSKPASRESFILAKWLTYSFSCIVLSLSIPAVVFFVQSQILWNQMPALLPFVK
                     GWLIMILQVQFYLALALLLGTIFNTRGPVTGIAMGFLLSGLILPNFIPQSVTLLFPWL
                     LMDLAAALALDNALPAVWPLPVIVTAIWTLIFIALALWRFRREEF"
     gene            1463..2629
                     /locus_tag="VFQ23_05985"
     CDS             1463..2629
                     /locus_tag="VFQ23_05985"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9906166.1"
                     /translation="MKSTKSVLEYHNVQNIISACLTVIILSLTIVGCGEQPVSVTQPP
                     TAIAGSPPSPTLELDESASKIDELLTSLTAKDEFTGAVLVARNGEILLSHGYGLADRD
                     NNLPNTPQTKFRLASITKEFTAAAILMLQEQHKLNVEDPICRYIPDCPAAWQEITIHH
                     LLSHTSGIPNITDFPDFSAFKATPSPPDQTIERFRNKPLDFEPGEKGSYSNSGYVLLG
                     TIIEQASGQSYESFLQKYIFEPVQMNNSGYDHNDGALATGYTGSRARWETPGDLDMSL
                     PFAAGGLYSTIEDLYLWDQALYTERLLSQESLDRMFTSQAMLSNLGMDYGYGWFIQDV
                     NNRRVLSHSGGIEGFATEMRRYPDDKVTIIILSNRDTTNVIAVGDQIARIVFTK"
     gene            2759..3655
                     /locus_tag="VFQ23_05990"
     CDS             2759..3655
                     /locus_tag="VFQ23_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9906167.1"
                     /translation="MTTISSVVPARESGLKAFAKRHPLTVYFVLAFALMWMFVLPIMF
                     SQRGLGIISLPEPLIEIFLLVATFSGPLPAALIVTTLLDGREGRRKLLRSMFQWRVGL
                     GWYLVLLVGYPLIFLTGLSFYSGAQPWVALIQNWPLLLSFYLPAAAFGIIFPSLGEEP
                     GWRGFALPRLQQQYGPLLGTVILGIIHGLWHLPVYFIPGMILEGPFDLTAVVANTGLL
                     IAMTLIWTWFFNNTGQSIFFAMLVHGVSNAASGFIPQLPLDVPADPWFSFKTGAAFAL
                     LLIVFTRGRLGYQKSRETEETR"
     gene            3809..4654
                     /locus_tag="VFQ23_05995"
     CDS             3809..4654
                     /locus_tag="VFQ23_05995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9906168.1"
                     /translation="MTTVTSALSSNESGLKAFIKRRPLISMYILLFLLGWPVQILDAL
                     ASHALAPKPPDILNIATGLTPAIAAIAVTAILAGRASVKDLFRRFLIWRVGVQWYLTG
                     LFLLAAVILGGIGLHVLFGGAMPEMPIAGAPVSLILFAFTVTVLVGALVNTEEIAWRG
                     FALPRLQSRYGALTAALLIAIPESLFHLPHFWNLNSVFYQNVGLFWFTAFTVAIVFIY
                     TYIFNKTNGSLLIVTLLHASQNAWANLLSDNTLRPFQFTVALMWMIAIALIFLTKGKL
                     GSENP"
     gene            4672..5463
                     /locus_tag="VFQ23_06000"
     CDS             4672..5463
                     /locus_tag="VFQ23_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9906169.1"
                     /translation="MSTYHQHEMYTQPHRATDAPAVSATNPEQHPLSLSILLHLLPGI
                     LTGAALFLLAPVTQQNGLPSLWAHGIADLIIILPFIFGLLFYLGYKRNGKFSLDGVVL
                     YRERIPLRHYLIFVPVVFLSAAIIPLLEPVSSFIFDRLFYWWPEMYNLSFDLRGYSAS
                     VIIATLIFNFLMVGVLVPVAEEIYFRGYLLPRLSRFGFWAVPIHTILFALFHVWTPWM
                     AIARAIGVIPLAYIAQRKKNIYIGIIVHILINTADVVTGIMLVLS"
     gene            5564..>6216
                     /locus_tag="VFQ23_06005"
     CDS             5564..>6216
                     /locus_tag="VFQ23_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906170.1"
                     /translation="MDSKTLHVLEYPKVLERLRAFCDFSASMELALALEPTDSYDLAL
                     ARLAETSEARKLFSTNDNIGVGGAHDIRPAVDLAARGGVLDPQQLLDIKSTLISCREL
                     KRSLERRTDEYPRLAQITAGLPESHGIVDAITRVLSERGEVLDSASPKLATLRREIKI
                     AHDRLMSRLQKYLTESGNKLQEPIITQRDGRYVIPLRAEFKGQIKAVVHDQSSSGAT"
BASE COUNT         1362 a         1653 c         1534 g         1667 t
ORIGIN      
        1 gacggcgcgc gaaacactgc gctttacagc ccgatttttt tattccggcc ctgaaaagcg
       61 aatggaagac cgcatcgacg aaacccttga gctggttggg ctatccgata aggcggatcg
      121 ctcgatcaaa ggcttttcgg gcggcgagcg tcagcgattg ggaattgccc aggcgcagat
      181 caattatcct gatctactaa ttctggatga acctgccgca tctctggacc cgatgggtcg
      241 ccgcgacgtg ctggaggtga tggagcgtct tcgcaagcat acgaccatct tctattcgac
      301 gcatatcctg gaggacgttc agcgggtgag tgataccgtc gcgattttga atcatggtcg
      361 ccttatcgcg caggcgccga tcgctgaact gacatccggc aaggatggca tcatctactc
      421 tttgaaggtt aagggagatg ggaaatgtgc gtatcagaat atccttggtc atccatgggt
      481 tgaatcagta accatggatt catccgactc aaataccaca ctgaatgtaa aagtaactga
      541 cgagcgccgg gctgagacgg aattgctgcg gctggcactc accgactcaa acacaatcgt
      601 gactcaattc ggacgcagaa agcataacct tgaggacgtt tttcttgaga tcgtcgaagg
      661 agacaaatca aatgaccacc ataactgaaa cactcactcc cagaacggaa cacggttggc
      721 gaatcggact caggaattta ctgtcgaacg aaatgaatct gcgctggggc ggtcgccgct
      781 ggatcaagtc tgcgttgatc tggctggcga tcctcaatgg ttttactttg ctggtttcgc
      841 ttgcagatga acaaaactca acctcgaaaa tcttagccga gaccattgat gtcttcgctc
      901 atgtgggact gctcgccacc tcgatcggcg ctgtcgttag tacacagggc gtgattattc
      961 gcgaaaagca attgggaaca gcggcctggg tcttatccaa acccgcttcg cgggagtcgt
     1021 ttattcttgc caaatggctt acgtattcct tttcatgcat tgttctgtcg ttgtccattc
     1081 ccgctgtagt cttctttgta caaagtcaga ttctgtggaa ccagatgcca gccttgcttc
     1141 ccttcgtaaa aggctggctg atcatgatac tgcaagtaca gttttatctg gctttggcac
     1201 tattgcttgg gacgatcttc aatacgcgcg gacccgtgac cggcattgca atgggctttc
     1261 tactcagcgg cttgattctt ccaaatttta taccgcagtc ggtcacgctc ctgttcccct
     1321 ggttgctgat ggatcttgcc gcagctttgg cgttagacaa tgctctgcct gctgtttggc
     1381 ccctaccggt tatcgtgaca gccatatgga ctttgatatt tatagccttg gcgctgtggc
     1441 gatttcgacg cgaggagttt taatgaaatc taccaaatct gttttggaat atcacaatgt
     1501 acaaaatata atctctgcct gcctgaccgt gattatcctc agcctcacga ttgtgggctg
     1561 tggggagcag ccagtatccg tcacgcaacc tccaacggcg attgcaggct cacctccctc
     1621 accgactttg gaattggatg agtcggcctc gaaaattgac gagctcctca catcactcac
     1681 tgcaaaggat gaattcacag gtgcggtcct ggtcgcccga aatggtgaaa ttttactcag
     1741 ccacggatat ggcctggcag accgcgataa taacctcccg aataccccac aaacaaaatt
     1801 tcggctggcc tcgatcacca aagagtttac tgctgcagca atcctgatgt tacaggaaca
     1861 gcacaagctc aacgttgagg atcctatttg ccgttacatc ccggattgtc ccgctgcgtg
     1921 gcaggagatt acgattcatc acctcctcag ccatacgtct ggaatcccga acattacgga
     1981 tttcccggac ttctcagctt ttaaagccac accttctcca ccagatcaga caatagaacg
     2041 cttcaggaac aagccattgg atttcgaacc cggcgaaaaa gggagctaca gcaattcagg
     2101 ctatgtgctg ctgggtacca tcatcgaaca ggcctccggt caatcgtatg agtcgttcct
     2161 ccagaaatat attttcgagc cggtacagat gaacaatagc ggatacgatc ataatgatgg
     2221 agcattagca acgggataca ctgggtcccg cgccagatgg gaaacaccag gcgatcttga
     2281 tatgtctctt ccctttgcag ccggagggct gtattccacg atcgaagatc tgtacctttg
     2341 ggaccaggct ctctacactg agcgactgtt atctcaagag tcgttggata ggatgttcac
     2401 gtcacaagct atgctatcga atcttgggat ggactatggc tacggttggt ttattcaaga
     2461 tgtgaacaat cgccgggtcc tcagccatag cggcgggatc gagggctttg ccaccgaaat
     2521 gagacgttac cctgatgaca aggtaaccat cattattctc agcaatcgcg acacaacgaa
     2581 tgttatcgcc gttggtgatc aaatcgcccg gatcgtattt actaaatagg aatatgttca
     2641 catcgcgctg ggaacctttc ccagcgcgat ttccctttaa ttcatgaacc tgccctcatg
     2701 tccacaggaa acgacagttg tatcctgtag tcaattcaaa cacaaaggag attgaaccat
     2761 gactacgatt tcttccgtcg ttcctgcccg tgaaagcgga ctgaaagcct tcgcaaaacg
     2821 ccatccgcta acggtctact tcgttctggc gtttgcactc atgtggatgt ttgttctccc
     2881 gatcatgttc tcgcaaagag ggctggggat tatcagtcta cctgagccgc tgatagaaat
     2941 attcctcctg gtcgctacgt tttctggacc cttgcccgcc gcgctcatcg tgaccacgct
     3001 actggatggt cgtgaagggc gacgtaagct gctgcggagc atgtttcagt ggcgcgtggg
     3061 gcttggctgg tatctcgtat tattagtcgg ttacccgctc atcttcctga ccggtttgag
     3121 cttttactcg ggcgcccagc cctgggtcgc gctcattcag aattggcccc tcctcctcag
     3181 cttctatttg ccagctgccg cgtttggaat catctttccg agccttggcg aggaacccgg
     3241 atggcgcggc tttgcactgc ctcggcttca gcagcaatat ggtccgttgc tcggaactgt
     3301 gatccttggc atcatccatg ggctatggca cctgcccgtc tacttcatcc ccggtatgat
     3361 cctggaagga ccgttcgatc tcactgcagt tgtagccaat acgggcctgc tcatcgccat
     3421 gaccctgatc tggacgtggt tctttaacaa tacgggtcag agcattttct tcgcaatgct
     3481 tgttcatggt gtgagtaacg ctgcaagcgg cttcatccca caactcccgc ttgatgtgcc
     3541 agccgaccct tggttcagct tcaagacggg agccgccttt gccctgctgc tgatcgtctt
     3601 cacccgcgga cgtttgggct atcaaaaatc ccgcgaaacc gaagagacgc gatgatctga
     3661 tgcagccgca ctcccataca acaaactcat gtagcgctgg gaaatcttcc cagcgcgatt
     3721 tccttttcat ttcgtgagcc tgtcctcatg tcgatgggaa acggaagttg tattctgtac
     3781 tcaatcttac aagcaaagga aatagacaat gactacagtt acttctgctc tctcctccaa
     3841 tgagtcaggg ttaaaggcct tcatcaaacg ccgcccgttg atctcaatgt atattcttct
     3901 gttcctgctg ggatggcccg tgcagattct cgacgcactt gcttcccatg cattagctcc
     3961 aaagccgcct gatattctca atattgccac ggggttgacg ccagctatcg ctgcgatcgc
     4021 agtgaccgct attctggcgg ggcgtgccag tgtaaaagat ttattccgcc gctttttgat
     4081 ctggcgcgtg ggcgttcaat ggtatctcac tggtcttttc ctgctcgcgg cggtcatcct
     4141 gggtgggatc ggactgcacg tgttgtttgg cggtgcaatg cctgagatgc ccatcgccgg
     4201 cgctcctgtg agtttgattc tcttcgcatt cacggtgacg gtactggtcg gtgcccttgt
     4261 caatacggag gagattgcct ggaggggttt tgccttgccc agactccaat cccgctatgg
     4321 cgcgttgacc gccgccttat tgattgcgat ccccgaatcc ctgttccacc tgcctcactt
     4381 ctggaacctg aatagtgttt tttatcagaa tgtgggcttg ttctggttca ccgctttcac
     4441 tgtggcaatc gtgttcattt acacatacat cttcaacaag accaatggca gtctcttaat
     4501 tgtgactctc ctgcacgcct cgcaaaatgc ctgggcgaat ttactgtcag ataacacact
     4561 gcgaccattc caattcaccg tcgccctgat gtggatgatc gcgattgcgt tgatctttct
     4621 cacaaaaggc aagttgggat ccgaaaatcc gtaatctttg gaggagccga tatgtcaacc
     4681 tatcaccagc acgaaatgta cacccagccc catcgtgcaa cggatgcgcc agccgtgtct
     4741 gcaacgaacc ccgaacaaca tccgctttca ttatcgatcc tgttgcattt actgcccggg
     4801 attttaactg gtgctgcctt gttcttactg gcacctgtca cccagcagaa tgggttgccc
     4861 tccctgtggg cgcatggaat tgctgacctg atcatcattc tgccctttat ctttggactc
     4921 cttttttatc tgggctataa gagaaacggc aaattctctt tggatggagt ggttctatat
     4981 cgcgaaagaa ttcccctgcg gcactatctg atttttgtgc cagtcgtgtt tctttccgct
     5041 gcgatcatcc cgttgcttga accggtgagt agtttcattt tcgatcgtct gttttactgg
     5101 tggccagaaa tgtataacct gtcatttgat ttgagaggct attcggcatc tgtcatcatt
     5161 gccacattga tctttaattt tctgatggtc ggcgtgctgg ttcccgttgc agaggaaatc
     5221 tattttcggg gttacctgct gccgaggctt tctcgcttcg gtttttgggc ggtcccgatt
     5281 catacgatcc tctttgcgtt gttccatgtc tggactccgt ggatggcgat tgcgcgcgcg
     5341 atcggtgtga ttcccctggc ctatattgcc cagcgaaaaa agaacatcta catcggtata
     5401 attgtccaca tcctgatcaa tacggcggat gtggttacgg gcatcatgct tgttctcagt
     5461 taatcgaacg attgatgttc aaaagattac gatcagaaag gtggactcat aatccgcctt
     5521 tttttatttt ctctattgtg tctgtggttt ataatttgcc ataatggatt caaaaactct
     5581 gcatgtcctt gaatatccaa aggtcctcga acgattaaga gccttttgtg atttctctgc
     5641 ctcgatggaa ctggcgcttg cgctcgagcc gaccgattcc tatgaccttg cgcttgcacg
     5701 cctggctgag accagcgaag cgcgcaaatt attttctacg aatgacaaca tcggtgttgg
     5761 tggcgcgcat gacattcgcc cggcggtgga tttggcggcg cgcggcggtg tgcttgaccc
     5821 tcagcaactt ctggatatca aatccacgtt gatctcgtgc cgcgagttga aacgatcgct
     5881 cgaacgccga acggatgaat acccgcgtct cgcgcagatc actgcgggat tgccagaatc
     5941 acatggcatc gtcgacgcga tcacgcgcgt gctctcggag cgcggcgaag tgctggattc
     6001 cgcttccccg aaacttgcca cgctgcgacg cgagatcaag atcgcgcatg accgtttgat
     6061 gtcgcgtttg caaaaatatc tcaccgagtc tgggaacaaa ctacaggagc ctatcatcac
     6121 ccagcgcgac gggcgatatg tgattcccct gcgcgcggaa ttcaaaggtc agatcaaggc
     6181 agtcgtccac gatcaatcct ccagtggtgc gacgtt
//
LOCUS       DASTJP010000198        15967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_583720, whole genome shotgun sequence.
ACCESSION   DASTJP010000198 DASTJP010000000
VERSION     DASTJP010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15967
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583720"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3993)
                     /locus_tag="VFQ23_06010"
     CDS             complement(<1..3993)
                     /locus_tag="VFQ23_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HET9906171.1"
                     /translation="MFKRIKISQFIFSTIMILSVGLSTTGPQPVLARSEDGIKRQLNA
                     ESGKVSFIGPENGRVVSAAEALGTFIRPQDPAMALAKRFGPEFGLKNPERDLSIKKSQ
                     QLESGQLTVRYQQKYQGIPVMGGELIVNTNLNGDLFSINGEVSPDLSLSTEPTITSEQ
                     AKQTALQSVAKWYQKPATDFIATEPELWIYDESLLRSSTRPTELVWRMEVTSKDQSLP
                     VRELVLVNAQRGNISLHFNQIDTAWHSSESAQRNQSPDSRASELDSEMKTSRNMANAP
                     HALLATTWYVATTGNDANSCSTTGSPCGTINGAIGKAANGDTIKVATGTYLGTGAQVV
                     LINKSITLSGGWNAGFSAQNGMSTINGQGERWGVAINSGVSAIIERSVVQNGKNSIGG
                     GIYNAGGTLSLNNSTISGNHATGGGGGGIFNNPGGQVTLNNSTVSSNNGTGIYNNGLP
                     WDNGTTTALTLNNSTVSSNNGTGIYNGGLGIIALNSSTVSNNTTAAGGGGIDNLDGSV
                     TMKNTLLAGNSSANSGQDCTGVIASSGYNLIGNTSGCSLAMSTGDQTNVNPNVGPLVG
                     TPGYHPLRAGSPAINAGNPAGCIGSTGTLSTDQRGASRVGTCDVGAYEYTSPGSVVSL
                     WIVNGNNQSTAPTLAFPLPLSVMALDSIGSPVPGVSITFTAPASGASGTFASTGARTT
                     SVNTDTSGVATTSTFTANNQLGSYLVSAATSGAGSVNFSLQNATWYVAPTGNDSNSCF
                     TSLSPCRTINGAIAKAANGDTIQVASGNYIGTGTAVVLINKSVVLSGGWNAGFTTQSG
                     MSIIDGQGARPGVSQEPNTSLSIERFVVQNGYSQYGGGIITSGSSLVLNEVLVQNNTC
                     YCFSGDGGGGIAIWGGALVTVTNSTITGNYAGSFGGGIFNKNGTLTLNNSTISGNDAD
                     GEAGGGIFSYSTVTLNNTTVSGNNGGGIYFYNDSGGAITFKMKNSLVANNWATSGPDC
                     KGHSMDSLGYNLIGNNSGCTFNSTTGDLVGTAANPINARINRLQDNGGVTFTHALGIG
                     SPAVNAGNPVTPGSGGNSCLGTDQRRATRPVGGRCDIGAYEGSVTWVPSPYVNTYTSG
                     NSSSLPGTLVCTQIDPNCSSGDAHAKAAHKYAIGTHDFFATQHDRISIDNNGMTIISS
                     VSYCSQSSCPYNNAFWDGTQMVFGSGFSLADDVVAHEFTHGVTQYESNLFYFYQSGAI
                     NESFSDLWGEYYDQSNGLGNDTAGVKWLQGEDVSGLGAHRSMIHPPAYGDPDKMSSTN
                     YYEGEDDSGGVHTNSGVNNKAVYLMVDGGTFNGKTVTALGWTKTAAIYYEVNTNLLSS
                     GADYSDLYFAL"
     gene            4423..5196
                     /gene="sufC"
                     /locus_tag="VFQ23_06015"
     CDS             4423..5196
                     /gene="sufC"
                     /locus_tag="VFQ23_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET9906172.1"
                     /translation="MSQLEIKNLHVSIDGKEILKGLDLTVEKGKVHAIMGPNGTGKST
                     LAYTLMGHPNYTVTEGEVHFKGQNVLELEPDERSRLGIFLAFQYPVAIPGVTVANFLR
                     TAINSRRRAENPEDKGIPIPEFRKLLKEQMTLLKMDHNFAGRYLNEGFSGGEKKRAEI
                     LQLATLKPEVAVLDETDSGLDIDALRIVADGVNALMNKEMGVLVITHYQRLLNYIKPD
                     YVHVMLDGRIVESGGPDLALHLEDQGYDWVREKHEETPA"
     gene            5279..6688
                     /gene="sufB"
                     /locus_tag="VFQ23_06020"
     CDS             5279..6688
                     /gene="sufB"
                     /locus_tag="VFQ23_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET9906173.1"
                     /translation="MSEDAKILEDIGQYKYGFHDRDDNYTFKSQKGLSREVVEQISHM
                     KGEPEWMLEFRLKALEHFQKRPMPNWGPTLKDLNFDEIYFYVKPTEQSEKSWDDVPDD
                     IKRTFDKLGVPEAERKFLAGLGAQYESEMVYHSIQEHLEKQGVIFLSIEDGLKQHPDL
                     FREYFGTVIPIEDNKFAALNSAVWSGGSFVYVPKNVKVDLPLQAYFRLNTANLGQFER
                     TLIIVEEGASVHYVEGCTAPQYTTDSFHSGVIEIIVKKGARSRYSTIQNWSTNVYNLV
                     TQRAKVFENASHEWVDANIGSKVTMKYPSCYLMEPGARGEMLSMAFAGPGQTQDAGSK
                     MYHFAPNTTSKITSKSISKGGGRASYRGLLKVYKGAKGVKSNVVCDALLLDPQSRSDT
                     YPYIEIDEDDVTIGHEASVSKVGEEQLFYLMSRGLSEEEATTMVVSGFIEPLVKELPM
                     EYAVEMNRLIALQMEGSIG"
     gene            6690..8030
                     /gene="sufD"
                     /locus_tag="VFQ23_06025"
     CDS             6690..8030
                     /gene="sufD"
                     /locus_tag="VFQ23_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET9906174.1"
                     /translation="MQEKPKVVVSRTRRAESTNKEFNFTESDVRTTGNGIASYRTVAW
                     NAFKRLALPGNTEEAWRRTDIHNLPVDKFSFAQEGMFNDLPAVREDLLKPLTGDQHGG
                     QIILMPGGVKVELNESLAKKGVIFTDLKTAEQKYPSLLAKMLGKTVNVEEGKFSALAG
                     AFAQTGVVLYVPKGVTVEEPLHSVLWGPGMNIAHVSHILVLVDEGASVTYVHESASPD
                     DIGSNSMHAGIVEIQVMQDASMKFVELQSWGRHVWNFSHERAHVERGGNLDWIFGAIG
                     SRLTKNFSELDLAGQGAQGRMSGFYFTDGNQHLDHDTQQNHLAPNTTSDLLFKGALKG
                     KSRSVWQGMIYVAPGAQKTDGYQANRNLVLSDAARADSIPGLEILADDVRCTHGATVG
                     KLEQEPIFYLKSRGIPQAEAEKIMVEGFFDPIFQRIPFDGVRERFQQYIADKMS"
     gene            8066..8527
                     /locus_tag="VFQ23_06030"
     CDS             8066..8527
                     /locus_tag="VFQ23_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906175.1"
                     /translation="MATKGKTKRSVQAKPSTSASVKEKPRLSEKVRVVNRISPPHVSS
                     KGEERKSSKGPRVPQFAYMKAPGLAHDFQVGDTVEVYCDHEKNKERVRGWVQGIVVQV
                     DNKLVAVQFRSNVFLTDGWMVPDRILWYPLNSEHIRPVLKKGKKERAIPDY"
     gene            8591..9814
                     /locus_tag="VFQ23_06035"
     CDS             8591..9814
                     /locus_tag="VFQ23_06035"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET9906176.1"
                     /translation="MLNVNEVREDFPILRRITHDDRPLVYLDSTATSQKPLAVIQAMD
                     NYYRQSNANIHRGVHTLAEEATTLYEQAREKIASFINSPSARQIIYTRNTTESINLVA
                     YSWARANLKAGDLVILTEMEHHSNLVPWQILQLEREIELDFIPVTEEGLLDLEAYKKL
                     LSRRPRLVSFTHMSNVLGTINPAAEIIRLAHAAGAVTLVDAAQSVPHLKVDVQALDAD
                     FLAFSAHKMCGPTGIGALYGKAELLEAMPPFLGGGDMIKEVHLRSFRANSLPHKFEAG
                     TPAIAEAVGFGAAVDYLSEIGMENIAAHEHEITEYALERLEEIPGLRIFGPSADKKGG
                     VAAFTLEGVHPHDVAQILDRDGIAVRAGHHCAQPLHEKFGLPATSRASFYLYNTKEEV
                     DLLVNGIYKVKELFG"
     gene            9973..10368
                     /locus_tag="VFQ23_06040"
     CDS             9973..10368
                     /locus_tag="VFQ23_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET9906177.1"
                     /translation="MDDLYREVIIEHYKHPSYKGHLDPHDISFEDSNPLCGDHIEITL
                     RTDERNTIADARFDGRGCAISQASADLLVESIIGKPLDEIKQLNKEHILNLLGIDLGP
                     VRLKCALLSLKVLKAGVYGLGEASDSLVE"
     gene            10384..10782
                     /locus_tag="VFQ23_06045"
     CDS             10384..10782
                     /locus_tag="VFQ23_06045"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET9906178.1"
                     /translation="MDDEPFYRLLSIVHGQNMFNYTTFDESKIEYYEIAPADELPPGE
                     RLFIEIGGQSLVIFNIAGQFFSIADICSHDDGPVGEGDLEGYNVVCPRHGGEFDVRTG
                     EAVQLPVVVDIPAYPIQVREGTIFVGIPKE"
     gene            complement(10831..11700)
                     /locus_tag="VFQ23_06050"
     CDS             complement(10831..11700)
                     /locus_tag="VFQ23_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906179.1"
                     /translation="MLSPNIPDFDSLWDYSQPDKTEERFRETILQIPEDDPAHLELLT
                     QIARAQGLQRKFDRAHQTLNQVEKRLSEYPSRAKVRYLLERGRVFNSSGNPHEARLFF
                     EQALDSAKQINEDFYAVDAIHMLAIISSPDTALALNLEAIRMAEASSQEKAQSWLGSL
                     YNNTGWSYHDLGNYERALEIFEKGEAWQRSNQRVSQTRIATWTVARTLRSLNRIQEAL
                     SKQLSLQDELESAGEEGDGYVYEEIGECLLALNRVDEARPFFAKAYDKLSKDEWLVKQ
                     EPHRLARLKELGV"
     gene            complement(11678..12934)
                     /locus_tag="VFQ23_06055"
     CDS             complement(11678..12934)
                     /locus_tag="VFQ23_06055"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9906180.1"
                     /translation="MNNTNNNWAVRYFTLWIGQALSLLGSQLVQFAIIWYLTQSTNSA
                     TTLAIASMMGLLPQVLLSPFIGTWVDRGNRRLIIIIADTTVALATVGLAILFALQIVE
                     PWHIYLALFVRAVASGFHEFAFGASVVLLVPKEQLARVQGFNQALYGALSIISAPLGA
                     FLLSVLPMQGILGIDVSTALLAVTILLFIQIPQPERAAQTQSTFLQDFVAGFRYILAW
                     RGLMILLGLAMVINFFFSAAEPLTPLLITNHFKGDVQQLAWWLSSFSIGTVLGGLILG
                     AWGGFKRKIVTSQVGLILSGLSTIVVGIVPSNQFWLGLVANTMVGLLLPIINGSFGAT
                     LQASIKPEMQGRVFSFIRSAAMLVSPLALVVAGPFADAFGIQLWFLIAGISCTLMGVA
                     GFFSAEVMGMEHYAHQESAHVVSEHS"
     gene            complement(12931..13278)
                     /locus_tag="VFQ23_06060"
     CDS             complement(12931..13278)
                     /locus_tag="VFQ23_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9906181.1"
                     /translation="MKIGHIELFVEDATASKDFYESVLGFEVVAVQSPDQIWVGCGEM
                     EILLRKGNPTNSNRYETSNNGIVLYTDDLESASQALANRGLTFKGTVDTNKCMAFTDL
                     DGHWFQLVNPNDL"
     gene            complement(13275..14348)
                     /locus_tag="VFQ23_06065"
     CDS             complement(13275..14348)
                     /locus_tag="VFQ23_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906182.1"
                     /translation="MKNYYRYYVWSINGFLIPMLLLVGCASAAPTSTSTSVQATLAPA
                     TLTATPSPDNSPTPESQPTLEAGQWTFLFYHEGLEQVILVNGGPERGKPADDPLELWS
                     WDGMKWSQVSADENGPAWRNFVPVAYDSVRDVLIFHSDKTWEWDGHTWRSFTGTGPGE
                     REGALMAYDVGRAKVVLFGGAIDMEVKSDTWEWDGEGWKQVSETGPAPRFPGGMIYDP
                     LRQEVLMYSGHFAESTGEFVDYDDLWGWNGEAWTFVNTDGPTPGHRNHTALVFDPMRE
                     QILLIGGRDLNLPGDTWIWDGVNWEEIPATNLPARSGHSVAYDPLRDKFILFGGVDRP
                     GGKALTDTWEWDREIWTCVENCQ"
     gene            complement(14426..>15967)
                     /gene="pepF"
                     /locus_tag="VFQ23_06070"
     CDS             complement(14426..>15967)
                     /gene="pepF"
                     /locus_tag="VFQ23_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET9906183.1"
                     /translation="YGKIQSVYGQAFAGLSFIEPEILAIDRKQLNKWRKEEKRLKPYA
                     QYFDNLFRQQAHVRSAEVEEVFGLALDPLGTLYTNMNLMTNSEMQYPNARTSDGKELT
                     VTQGSLDEILASPDREARRTAWDGFRDTHLAFKNTLANNLVGSVKANVFAQRVRKYPS
                     TLEGSLFVNNVPEKVFHNLIETTRKNLPVWHKYWRVRREALNQQEIQPYDVWAPLTQN
                     PPKISYEQSVEWILDGLAPLGEEYVSTARRGLLQEGWVDVYPNLNKSGAQFSSGSKGT
                     HPFIVMLYDDTIFGLSTIAHELGHSMHSYLTFKHQPMVYAGYTLFVAEVASNLNQALV
                     RAHLLKTNTDPEFQISVLEEGFANFHRYFFIMPLLAQFELEVHRRVERGQGVTADDMN
                     AIMADLYAEGYGSEMQFDRDRTAITWATFGHLYQDYYVYQYSTGISGAHALSRRILSG
                     VPNAAEDYLRALSAGNSMYPLDTLKLAGVDMSTPRPIEETFEILSEMVDRLEELTKEK
                     TVAAT"
BASE COUNT         4054 a         3983 c         3957 g         3973 t
ORIGIN      
        1 gagggcaaaa taaagatccg agtaatcggc accggacgag agcaggttgg tattcacctc
       61 gtaataaatg gcagcggtct tggtccaacc caaagcagtg acagttttac cgttgaacgt
      121 tcctccatcg accatcaggt agacagcctt gttattcaca ccgctatttg tatgaacacc
      181 accactgtca tcttcacctt catagtaatt ggtactggac attttatcgg ggtcaccata
      241 agcaggcgga tggatcatgc tgcgatgggc acccaggccg gagacatcct cgccttgaag
      301 ccacttgacg ccagcggtat cgttgcccaa tccattactt tgatcgtaat attcgcccca
      361 taaatccgaa aatgattcgt tgatcgcccc ggattgatag aaatagaaca gattggactc
      421 gtattgtgtc accccatgag tgaactcatg cgctacaaca tcatctgcta gagaaaatcc
      481 gctaccgaac accatttgtg ttccatccca aaaggcgtta ttgtatgggc aggaagattg
      541 gctacagtag ctcacgcttg aaatgatcgt cattccgtta ttgtctatgc tgattcgatc
      601 atgttgagtg gcaaagaaat catgtgtacc gattgcatac ttatgggctg cctttgcatg
      661 agcatcgccc gaactgcaat tcggatcgat ctgagtacac accaaggtac caggcaggga
      721 tgagctgtta ccggatgtat aagtattaac gtaaggcgaa ggtacccatg ttaccgaacc
      781 ttcgtaggca ccaatatcgc agcgtcctcc aacaggtcgt gtagctcgac gctggtcagt
      841 tcccaaacat gaattacctc cacttccggg cgttactgga ttacctgcat taacggcggg
      901 actgccaatt cctaatgcat gcgtaaatgt aacgccacca ttatcttgca agcggttgat
      961 gcgagcattg ataggatttg cggctgtacc tacaagatca cctgtagttg aattgaatgt
     1021 gcaaccggaa ttatttccaa tcaagttata tccgagcgag tccatggaat gacctttaca
     1081 gtctggacca gatgttgccc agttattagc tactagggaa tttttcattt tgaaggtgat
     1141 tgcaccacct gagtcattgt aaaagtaaat accgccacca ttattcccac taacggtagt
     1201 gttgttcaac gtcaccgtgc tataactgaa tattccacca ccagcctcgc catccgcgtc
     1261 attgccgctt atggtactgt tattgagcgt tagcgttccg tttttgttaa aaattccccc
     1321 accaaaactt ccggcgtaat tgcctgtaat tgtgctgttg gttactgtga ccaatgcgcc
     1381 gccccagatc gcaatgcccc caccgccgtc accactaaag cagtaacacg tgttattctg
     1441 gacgagaact tcattcaaaa ctagggaact gccactcgta attattcctc caccatattg
     1501 tgaataacca ttttgtacca caaatcgctc aattgaaagt gatgtgtttg gttcttggct
     1561 gactcctggg cgcgctcctt gtccatcaat tattgacatc ccgctttgag tggtaaaacc
     1621 agcattccac ccgcctgata aaacaacgct cttattgatc aacaccactg ctgtgcctgt
     1681 tccaatatag tttccactgg ccacttgtat cgtatccccg ttcgctgctt tggcaatggc
     1741 gccattgatt gttcggcagg gtgatagaga agtaaaacaa gaattcgagt cgttaccagt
     1801 gggcgccaca taccatgtag cattttgaag gctaaaattg actgatccag ccccgcttgt
     1861 agcagcagaa actaagtatg accccaactg attgttggct gtaaatgtag atgttgttgc
     1921 aacaccactt gtatccgtgt taacagaagt tgttcgggcg ccagtgcttg caaatgttcc
     1981 gctggcccca ctggctggtg ccgtaaaggt gatactcact ccaggaacgg ggctgccgat
     2041 actatcaaga gccatgacac ttaatggcag agggaaagcg agtgttggcg cagtgctttg
     2101 gttgttgccg ttcacaatcc aaagactgac cactgaacca ggtgaagtgt attcataagc
     2161 acccacatca catgtaccca cccgagatgc accgcgctga tccgtgctaa gtgtgcctgt
     2221 gctccctata caacctgccg gattgccagc gttaatagct gggctaccag cgcgtaaggg
     2281 gtgataacct ggtgttccga ctaagggccc aacattgggg ttaacatttg tctgatcgcc
     2341 cgtactcata gcaaggctgc accctgacgt gttgccaatc aggttatacc cgcttgatgc
     2401 aattacgcct gtacaatcct gcccactatt cgcagacgag ttgccggcca gtaaggtatt
     2461 tttcatggta acagagccat cgaggttatc gataccgcct ccgccagcag cggtggtgtt
     2521 gttactgact gtgctgctat ttaacgcgat tatgccaagc ccaccattat atatgccagt
     2581 cccgttattg ctactaacgg tgctgttgtt caatgtcaaa gcagtcgtag taccattatc
     2641 ccaaggcagg ccgttgttat atattccagt cccattgttg ctgctgacgg tgctattgtt
     2701 gagtgtcact tgcccgcctg ggttgttaaa aatgcctcca ccacctccac ctgtggcatg
     2761 attaccgctg attgtgctgt tattcagtga taatgtcccg cctgcgttat aaatcccacc
     2821 gccgatggaa tttttaccat tctgcactac gcttcgctcg ataatcgcag aaacaccgct
     2881 atttatcgcc acaccccagc gctcgccctg tccattaatc gtggacatgc cgttttgagc
     2941 agaaaaacct gcattccaac caccggagag agttatgctt ttatttatga gcactacttg
     3001 cgcgccagtt ccaagataag ttcccgttgc tactttaatc gtatcgccat tcgccgcttt
     3061 gccaatcgct ccattgattg ttccacaggg acttccagta gtagaacatg aattggcatc
     3121 gtttcccgtt gttgccacat accaggttgt agcaaggagc gcgtgagggg cattggccat
     3181 gtttcggctt gtcttcattt ctgaatctaa ttctgaagcc ctggaatcag gagattgatt
     3241 tcgctgcgca ctttctgaag aatgccatgc ggtatcgatc tgattgaaat gcaggctgat
     3301 gttgccgcgc tgagcattga ccagcaccag ctcccggaca ggaaggcttt ggtcctttga
     3361 agtcacttcc atacgccaaa cgagttcagt aggacgggtg cttgaacgta agaggctttc
     3421 atcatagatc catagttcag gctctgttgc aatgaaatct gtggccggct tctggtacca
     3481 ctttgccaca gattgaaggg cagtctgctt tgcttgttca gaagtaattg ttggctcggt
     3541 cgaaagagac agatccggcg agacttcccc gttgatcgaa aataagtcgc cattcaagtt
     3601 tgtgttgacg atcaactctc cgcccatcac gggaataccc tgatatttct gctggtaacg
     3661 gacagtcagt tgcccactct ccagttgttg ggattttttt atagaaaggt cacgctctgg
     3721 attcttcaac ccaaactcag gaccaaatcg tttcgcaagc gccatagccg ggtcttgtgg
     3781 acgaatgaag gttccgagcg cttcggcggc agatacgacg cgtccgtttt cgggaccgat
     3841 aaaactgacc ttgccactct ctgcgttgag ttggcgtttg atcccatctt cgcttcgggc
     3901 gaggacaggc tgaggtcccg tcgttgaaag ccctacagac aaaatcataa ttgtagaaaa
     3961 aatgaactga ctgattttaa ttcgtttgaa cattcacatc tccgagaatt gaatttggac
     4021 gcgacaaaaa cgagccgaat gattttggaa ccgcatatgt ttcacgccat atcaagaaga
     4081 attcattgtt tttaggaaag cctcttcaaa gattgccacc ctaacctaat cttgggagtt
     4141 gcatgaactt aaggttagct actccaagac ggaaggtttc tgtacctgtt acgttaaatc
     4201 atttagaaaa ggttacagag ttggaacgcg caaggcaatt tattacaaaa aaaccagtaa
     4261 aactgtgacc tcgattatca gtgaatatac tagaaaaata tcataaatat tgactttttc
     4321 ccctattcct gctataatgc cgccgtctgg cggtagatga gcgaaggtgg tcgtcttagg
     4381 ttgatttgtc agacaaataa caagaattgg tcaggagatt caatgtcgca actggaaatc
     4441 aaaaaccttc acgtgagtat cgacgggaag gaaatcctca aaggtctcga tttaacggta
     4501 gaaaaaggga aagtccacgc tatcatgggt cccaatggga cgggaaagtc aacgcttgcc
     4561 tatacattaa tggggcaccc caattacaca gtcactgagg gagaagtaca tttcaaagga
     4621 cagaatgtgc tcgagctgga accggatgaa cggtcccgac tcgggatttt cctggctttt
     4681 cagtacccgg ttgccatccc cggtgtgacg gtcgcgaatt ttctgcgaac cgccatcaat
     4741 tccaggcgcc gcgccgaaaa tccggaagat aaggggattc cgatccctga attccgtaaa
     4801 ttgctcaagg agcaaatgac tctccttaaa atggaccaca actttgccgg acgctatctc
     4861 aacgaaggat tttctggtgg agagaaaaag cgtgccgaga tcttgcagtt ggccacgctc
     4921 aagcctgaag ttgcagtcct ggatgaaacc gactcaggcc tcgacattga tgcactccgg
     4981 atcgtcgcag acggcgtcaa cgcgctgatg aacaaagaga tgggtgtgct agtcattact
     5041 cactaccagc gattgttgaa ctacatcaag ccggactatg tccatgtgat gctggatgga
     5101 cgaatcgtcg aatcaggtgg accggatttg gcgttgcatc tcgaagatca agggtacgat
     5161 tgggtgcgcg agaagcatga ggaaacgcct gcatagtgac tttagcacaa aggactcgaa
     5221 cgtcacgaag actttctgaa cattatgccc tttgtggtga atcagctcgg agattattat
     5281 gagtgaagat gctaaaattt tagaagatat cggtcaatat aaatatggtt tccatgaccg
     5341 cgatgataac tacacattca aatcgcaaaa aggtttgagc cgtgaagttg tagagcagat
     5401 ctcgcacatg aagggcgagc cagaatggat gctcgaattt cgcctgaaag cgcttgagca
     5461 cttccagaag cggccaatgc cgaactgggg tcccacgctg aaagatctca acttcgatga
     5521 gatttacttt tacgtcaaac ccacggagca aagcgaaaaa tcctgggatg acgtgcccga
     5581 tgatatcaag cgtacattcg ataaacttgg cgtgccggaa gccgagcgca agttcctggc
     5641 tggtttgggt gcgcagtatg aatccgagat ggtttatcac tcgattcagg aacatcttga
     5701 aaagcagggt gtgattttcc tgagcattga agacgggttg aagcagcatc ctgatttgtt
     5761 ccgcgaatat tttgggactg tcattccgat cgaagataat aaatttgctg cattgaacag
     5821 cgcagtgtgg tctggcggat cgtttgtgta tgttccgaaa aacgtgaagg ttgacttacc
     5881 tttgcaggca tacttccgtt tgaacacggc gaatcttgga caattcgaac gtacactgat
     5941 catcgttgaa gaaggtgcgt cggttcatta tgtcgagggt tgtacggcgc cacagtatac
     6001 gaccgactct ttccacagcg gcgtgatcga aattattgtc aagaaaggcg ctcgctcgcg
     6061 ttactccacc attcaaaact ggtcaaccaa cgtatacaat ctcgtcacgc aacgcgccaa
     6121 agtctttgaa aatgcatcgc atgaatgggt agatgccaac atcggctcga aagtcacaat
     6181 gaaatatcca tcctgctatc tcatggagcc aggggcgcgt ggcgaaatgc tctcaatggc
     6241 gttcgctggt ccagggcaga cgcaggacgc cggcagcaag atgtatcatt tcgcgccgaa
     6301 tacaaccagt aagatcacat caaaatctat cagcaagggt ggcgggcgcg catcctatcg
     6361 tggtctgctc aaagtctata aaggcgcgaa gggcgtcaag tccaatgtgg tctgtgatgc
     6421 gcttttactt gatccacagt cccgctcgga tacgtaccct tacatcgaga ttgacgaaga
     6481 cgatgttacg atcggacacg aagcgtcggt gagcaaagtg ggtgaggagc agctgttcta
     6541 tcttatgtca cgtggtctca gcgaagagga agcgaccacg atggttgtct cgggatttat
     6601 tgagccgctg gtcaaggaat tgccgatgga atacgcagtt gaaatgaatc gcctcatcgc
     6661 attgcagatg gaaggaagca tcggctaaca tgcaggaaaa acccaaagta gttgtttcaa
     6721 ggactcgtcg agccgagtcg acaaacaaag aatttaactt tactgagtct gatgttcgca
     6781 caacggggaa cggcattgct tcctatcgga ccgttgcctg gaatgctttc aaaagactcg
     6841 ctctcccggg aaacacagaa gaggcctggc gtcgcaccga tattcacaat ctgccggttg
     6901 ataaattcag cttcgcgcag gaaggtatgt tcaatgacct gccagctgtg cgtgaagatt
     6961 tgctcaaacc attgactggt gatcagcacg gtggacaaat catccttatg cctggtggcg
     7021 taaaggttga gcttaacgaa agccttgcaa aaaaaggtgt tatcttcacc gacctgaaaa
     7081 ccgctgaaca aaaatatccg tctttgctgg caaaaatgct tggaaaaacg gtcaacgtag
     7141 aagaaggtaa gttctctgcg ttggcgggtg catttgcgca gaccggtgtg gtgttatatg
     7201 ttccgaaagg tgtgacggtc gaagagccac ttcactctgt gctgtggggc ccgggtatga
     7261 atattgctca tgtctcgcat attcttgtcc ttgttgacga aggcgcgtcg gtgacttatg
     7321 ttcatgagtc tgcctcgcct gatgacatag gctccaattc gatgcacgcc ggaattgtgg
     7381 agatccaggt aatgcaggat gcgtcgatga agttcgtcga gttacagtca tggggacgac
     7441 acgtttggaa cttctcgcat gagcgtgcgc acgttgaacg tggcggtaat ttggattgga
     7501 tcttcggcgc gatcggctcg cgccttacga aaaatttctc tgagcttgac ctggctggac
     7561 aaggcgcaca gggacgcatg tcaggtttct attttacaga tggcaaccaa cacctcgacc
     7621 atgacacgca gcagaatcat cttgcgccga acacgacaag cgatctgcta ttcaaaggcg
     7681 ccctgaaagg caagagccgc tccgtctggc agggcatgat ctacgtcgct cccggagcac
     7741 agaaaacgga tggctatcag gcgaaccgga atctggtgct gagtgatgcg gctcgtgcag
     7801 attcgatccc gggtttggaa atcctcgctg atgatgtgcg ctgtacgcac ggcgccaccg
     7861 ttggaaagct cgaacaggaa ccgatatttt atttgaaatc gcgtggtatt ccccaggcgg
     7921 aggctgagaa aattatggtg gaaggatttt ttgatccgat tttccagcgc atcccgtttg
     7981 acggtgtacg tgagcgattc cagcaatata ttgctgacaa aatgtcatag tcaataaatc
     8041 tatccagaaa gaagtgaaac tattgatggc tacaaaagga aaaacgaagc gttcggttca
     8101 ggcaaaaccc agtacgagtg caagcgtcaa agagaaaccc aggttaagtg agaaggtgcg
     8161 tgtagtgaat cgaatctcgc ctccgcacgt ttcatcaaag ggcgaggagc gaaaatcatc
     8221 gaagggccca cgtgtgcctc aatttgccta tatgaaagca cctggtctcg ctcatgattt
     8281 tcaggtaggt gatacggtcg aagtgtactg cgaccatgaa aagaacaaag aacgcgtacg
     8341 gggctgggta cagggcatcg tggttcaagt tgacaataag ttggttgctg tgcaattccg
     8401 ctcgaacgtg ttcctcaccg acggatggat ggtacctgac cgtatccttt ggtatccatt
     8461 gaattctgaa catattcgtc ctgtcttgaa gaagggcaaa aaagaacggg caattccgga
     8521 ttattagcta acccgcacca ggaaggaaat tgagtgcctt cctgtgtgca tctatgcaat
     8581 cgaattccgc gtgttaaatg taaacgaagt tcgcgaagat tttcctatac tccggcgcat
     8641 cactcacgat gatcgtccgc tggtgtattt ggattctacc gccacctcac aaaagcctct
     8701 ggcggttatc caggcaatgg ataactatta tcgccagtca aatgcaaata ttcaccgtgg
     8761 tgtgcacaca ctggcagaag aggcgaccac tctgtacgag caggcgcggg agaagattgc
     8821 gagctttatc aattcgccct cggcacgaca gatcatctac acccgtaata caacggaatc
     8881 cataaatctt gtcgcctaca gttgggcgcg tgcaaatttg aaggcgggcg acctggtcat
     8941 tctgaccgag atggagcatc actccaatct ggtgccctgg cagatattgc agcttgaacg
     9001 agagatcgag cttgacttta tccccgtgac ggaagagggc ttgcttgatt tggaggctta
     9061 caagaagctt ctctcgcgac gcccgaggct ggtctcgttt acacacatgt ccaacgtcct
     9121 tggtacgatc aaccctgcgg cagaaatcat tcgacttgca catgcagccg gcgcggtgac
     9181 cctggtcgac gcagcacaat ctgtccctca tctgaaagtg gatgttcaag ccttggacgc
     9241 ggatttcctt gccttttccg cgcacaaaat gtgcggacca acgggtatcg gcgcgttgta
     9301 tggaaaagct gagttactgg aagccatgcc cccgttttta ggcggcggcg atatgatcaa
     9361 agaggttcat ctgcgttcct tcagggcaaa ttctttgcca cacaagttcg aagcagggac
     9421 gcctgccatc gcagaagcgg tcggtttcgg cgcggcagtc gattatcttt ctgagatcgg
     9481 tatggaaaac atcgctgccc atgaacacga aatcacggaa tacgcgctcg agcgactcga
     9541 agaaatcccc gggttgagaa tctttggacc ttcggcagat aagaaaggcg gcgtggcggc
     9601 ctttactctt gagggcgtcc acccacatga tgtggcgcag attttagacc gcgatggcat
     9661 cgccgtacgc gcgggccatc actgcgccca gcctctgcat gagaaattcg gtctccctgc
     9721 cacgagccgt gcttccttct atttgtacaa tacaaaagag gaagtcgatt tactggtaaa
     9781 cgggatttat aaagtgaagg aattgtttgg atagatccgt tggtcgagta gggcgacact
     9841 tgggcctgcg cgcagtgccg gtgagcccat atcgagacca acatgttaca aggaaactct
     9901 ggttaagtgg tggtcgagta gccctgagca gcatctgcga agggctactc gaccaccgag
     9961 aaataagatc atatggacga cctctatcgc gaagttatta ttgaacacta taagcatccc
    10021 tcttacaagg gacaccttga tcctcatgat atttcatttg aggatagcaa ccctttgtgt
    10081 ggcgatcata tagagattac tctgcggaca gatgagagaa acactatcgc cgatgcgcgc
    10141 ttcgacggtc gcggttgtgc catatcgcaa gcctccgctg atctgctggt ggaatccata
    10201 attggaaaac ccctcgacga aattaaacaa ctcaacaaag aacacatcct caatctgctt
    10261 ggcatcgacc ttggtcctgt ccgcttgaag tgcgccctgc tttccctgaa ggtgctcaaa
    10321 gcgggagtat acgggttagg cgaagcaagc gactccctgg tggaataaaa ccgacaattg
    10381 accatggacg atgaaccatt ttatcgtcta ttgtcaattg tccatggtca aaatatgttc
    10441 aactacacaa ccttcgacga atcaaaaatt gaatactacg aaatcgcccc ggcagatgaa
    10501 cttccgccgg gtgaacgatt atttatcgag attggtggtc aatcccttgt gatttttaat
    10561 atcgcagggc agtttttttc gattgccgat atttgctcgc atgacgacgg ccccgtaggg
    10621 gagggagatc tcgaaggtta taacgtcgtt tgcccgcggc atggcggcga gtttgatgtg
    10681 cgaacggggg aagccgtcca attgccggtg gtggtggata tccctgctta cccgattcag
    10741 gtgcgcgagg gaactatttt tgtgggaatt ccgaaagagt aaactttgac agcaaatgtc
    10801 ttttgtgaaa agcaaaagac atttttatat ttacacaccc agttccttca agcgcgccag
    10861 acgatgtggt tcctgtttca ctagccattc atccttcgac aatttgtcat acgctttcgc
    10921 aaaaaagggt cttgcttcat caacccgatt caaggctaac aaacattcac cgatttcttc
    10981 atagacatac ccatcgccct cttcacctgc ggactccagt tcatcctgca gtgatagttg
    11041 tttggataat gcctcctgga tccgattcaa ggagcgcagc gtccgcgcga cagtccacgt
    11101 ggcgattcgc gtttgactca cgcgttgatt agacctctgc catgcttccc ctttctcgaa
    11161 aatttcgaga gctctttcat agttgcccag gtcgtgatat gaccagcccg tattattgta
    11221 taaggagccg agccagctct gtgctttttc ttgagatgaa gcttctgcca ttcgaattgc
    11281 ttcgagattt aaagccaacg ccgtatcagg agaggagata attgccagca tatggatggc
    11341 atccaccgca taaaaatctt cgttgatttg tttcgctgaa tccaatgcct gttcgaagaa
    11401 caggcgtgcc tcgtggggat tcccggacga gttgaacact ctaccgcgtt cgagtagata
    11461 gcgtaccttc gcgcgggacg ggtattcgct caaccgcttt tcaacttgat tcaaagtctg
    11521 atgggccctg tcaaacttcc gctggagtcc ctgggcgcgc gcgatttgag tcaatagctc
    11581 gaggtgagcc ggatcatctt ctggaatttg aagaattgtt tcgcgaaatc tctcttctgt
    11641 tttatcaggc tgcgagtaat cccaaaggga gtcaaaatca ggaatgttcg gagacaacat
    11701 gtgcgctttc ctggtgggca tagtgttcca tgcccattac ctctgctgag aaaaacccgg
    11761 ccacgcccat caatgtgcag gaaatgccag cgatcaggaa ccaaagctgg atgccgaatg
    11821 catctgcaaa aggacccgca accacaagtg ccagtggtga aaccagcatg gctgcgctgc
    11881 gaatgaacga gaatacccgt ccctgcattt ccggtttgat gctagcctgc aacgtagcac
    11941 caaaggaacc attgatgatg ggtagtaata atccgaccat ggtgttggcg actaatccaa
    12001 gccaaaattg attggatgga acgataccaa cgacaattgt actcagcccg gaaagaatca
    12061 gccccacttg cgaagtgacg attttgcgct tgaagccacc ccaggcgcca aggatcaacc
    12121 caccgaggac agtaccaatg gagaaactag ataaccacca tgcaagttgc tgcacatcgc
    12181 ctttgaagtg attggtgatg agcaacggcg tgagcggttc ggccgcgctg aaaaagaaat
    12241 tgatcaccat tgccagcccc agtaaaatca tcaagccacg ccaggcaagg atataacgga
    12301 atccggccac gaaatcctgc aggaatgtgg attgggtctg cgctgcccgt tctggctgtg
    12361 ggatttggat gaatagcaga atcgtaaccg caagcagtgc tgtactgaca tcgatcccga
    12421 gaattccctg catcggaagg accgagagca ggaacgctcc aagtggtgca gaaatgatgc
    12481 tcaatgctcc atacaacgcc tgattgaatc cctgcacgcg cgccagctgt tctttcggca
    12541 ccaacaaaac caccgacgcg ccgaaagcaa actcatgaaa cccactcgcc acggcgcgga
    12601 caaacaacgc tagatagata tgccagggct ccacaatttg taaagcgaat aaaatcgcca
    12661 gaccaaccgt agccagcgcg acggttgtat ccgcaataat aatgatcaat cgccgattgc
    12721 cacggtccac ccaggtgcca ataaatgggg agagcaagac ctgaggcagt aatcccatca
    12781 tggacgcaat cgcaagcgtc gttgcagagt ttgttgattg ggtgaggtac cagatgatcg
    12841 cgaactggac cagttggctg cccagtaacg aaagtgcctg accgatccac aaagtgaaat
    12901 atcgaactgc ccagttgtta tttgtattgt tcataagtcg tttggattta ccagttggaa
    12961 ccaatgtccg tctagatcgg tgaacgccat gcacttgtta gtatctacag ttcctttgaa
    13021 tgtgagtccg cggttggcta atgcttgcga tgcgctttcg agatcatcag tatataaaac
    13081 gattccgtta ttggatgttt catatctgtt tgaattggtc ggattccctt tcctcaaaag
    13141 gatttccatc tcgccgcatc ccacccagat ctgatctgga ctctgcaccg cgaccacttc
    13201 aaaccctaaa acagattcgt agaaatcctt cgatgcagtt gcatcttcga caaatagctc
    13261 aatgtgtccg attttcattg acagttctcc acgcaagtcc aaatttcacg atcccactcc
    13321 caggtgtcag taagcgcttt tccgcctggg cgatccaccc cgccaaacag aatgaatttg
    13381 tctcgcagtg ggtcataggc aacactgtgt ccacttctgg ctggcaaatt ggttgcggga
    13441 atttcttccc aatttacgcc atcccagatc caggtatcgc ctgggagatt caggtctcta
    13501 ccaccgatca gcaggatttg ttcccgcatt gggtcaaata cgagtgcagt atggttacga
    13561 tgaccagggg tgggtccgtc ggtgttgaca aacgtccaag cctctccgtt ccatccccag
    13621 agatcgtcgt aatcaacgaa ctcacctgtt gattcggcaa agtgaccgct gtacatcaac
    13681 acttcctgcc ggaggggatc gtaaatcatg ccgcctggga agcggggcgc gggccctgtt
    13741 tcgctaactt gtttccatcc ttcgccgtcc cattcccatg tatcactttt cacttccata
    13801 tcaatcgccc cgccaaagag cacaactttc gcgcgaccaa cgtcgtaggc catcaacgcg
    13861 ccttcacgct cccctggacc cgtacctgta aaacttctcc acgtgtgtcc gtcccattcc
    13921 caggttttat cactatggaa aatcagcacg tcacggacgg agtcatatgc aactggcacg
    13981 aagtttcgcc aagccggacc attttcatcc gcgctaacct gtgaccactt catcccatcc
    14041 caactccaaa gttccaatgg gtcgtctgcg ggcttacctc gttcaggacc gccgttcacc
    14101 aggatgactt gttccaatcc ttcgtgataa aagagaaacg tccattgacc tgcctccagc
    14161 gtcggttggc tttcaggagt tggagaatta tctggagatg gagtcgctgt cagcgttgcc
    14221 ggagcgagcg tcgcttggac ggatgtagat gtagatgtag gtgcagccga tgcacatccc
    14281 accagaagga gcataggaat caggaagccg ttaatggacc aaacgtaata ccgatagtag
    14341 tttttcatgg aaacctccta cttattaatc tcaacaaata cacctgactt cagtaggagg
    14401 ttcctgcttg aaagcaaaca cactttcatg ttgcggcgac tgttttttct ttggtcagct
    14461 cttccaggcg gtcgaccatc tcggaaagga tctcgaatgt ctcttcgatt ggccgcggcg
    14521 ttgacatatc cacccccgca agcttaagcg tgtcaagcgg gtacattgag ttgcccgcac
    14581 tgagcgcgcg cagataatct tcggcagcgt ttggaacccc agacaggata cgacgtgaga
    14641 gcgcgtgcgc accagagatc ccggtggaat attggtagac gtaataatcc tggtacaaat
    14701 gaccaaacgt cgcccaggtg atggcggtac ggtcgcggtc gaactgcatc tcggatccat
    14761 agccctccgc gtagaggtct gccatgatgg cattcatatc gtctgccgtg acgccttgtc
    14821 cgcgttcgac gcggcgatgt acctccaatt caaattgtgc cagcagcggc atgataaaga
    14881 aataacgatg gaagttcgcg aatccttctt caagaacgct gatctgaaac tcagggtcgg
    14941 tgtttgtctt cagcaaatgc gcgcggacca aagcctgatt caaattcgat gcaacctcag
    15001 ccacaaacag cgtatacccg gcatacacca tcggttgatg cttgaaggtg agataggagt
    15061 gcatggaatg accgagttca tgagcaatcg tgctcaagcc gaagatcgta tcgtcgtaca
    15121 acatcacaat gaatggatgc gtgcctttgg agccggatga gaattgtgcg ccgctcttgt
    15181 tgagatttgg atacacatcc acccagcctt cttggagaag accgcgccgc gccgtagaaa
    15241 catattcctc tcccagcgga gccagcccat ccagaatcca ttcgacggat tgctcataag
    15301 aaatctttgg tgggttctgt gttaatgggg cccaaacatc gtaaggttga atttcctgct
    15361 gattcaatgc ctcacggcgc acgcgccaat acttgtgcca gaccggaagg tttttccggg
    15421 ttgtttcaat taaattatgg aatacttttt ccggaacatt attgacgaac aaagagcctt
    15481 ccagcgtgga tggatatttt cgcacccgct gcgcaaacac attcgctttg acggaaccga
    15541 ccaggttatt cgccagtgtg ttcttaaacg caagatgcgt atcgcggaaa ccgtcccatg
    15601 ccgtgcgcct ggcttcgcgg tcgggtgagg ccaagatctc atcgagtgag ccctgcgtga
    15661 cggttaactc ttttccgtca gaggtgcgcg cgttgggata ttgcatctcg ctgttggtca
    15721 tcaaattcat gttggtgtac agagtaccca gcggatccag cgcgagcccg aatacctctt
    15781 ccacctccgc agaccgaaca tgagcctgct ggcgaaacag gttatcgaaa tattgggcat
    15841 agggcttcaa tcgtttttcc tctttgcgcc acttgttaag ctgtttgcga tcgatcgcca
    15901 ggatttccgg ttcgataaac gacaaccccg cgaaagcctg tccgtacacg ctctggattt
    15961 ttccata
//
LOCUS       DASTJP010000199         5183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_7312,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000199 DASTJP010000000
VERSION     DASTJP010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7312"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFQ23_06075"
     CDS             complement(<1..472)
                     /locus_tag="VFQ23_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9906184.1"
                     /translation="MNLRSTLIITFTILLSACNFTLAEDITPPPNYISPTPAPTLALF
                     PAEMPNTETGAAIYVEKCAPCHGETGMGDGPQGIQLQGVTVPAFGLPEVARPASLARW
                     YTTVTRGNIERFMPPFASLSDQERWDVVAYAMTFHSTQEEIERGKALFEENCSNC"
     gene            complement(483..1067)
                     /locus_tag="VFQ23_06080"
     CDS             complement(483..1067)
                     /locus_tag="VFQ23_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9906185.1"
                     /translation="MDIAALFFTLAILVLVAMYLYAPLAQRFARPVTHEEHELSALMA
                     ERDRIINALQELDFDFKLGKIPDEDYPAQRTNLLQKGADVLRKIDNLTPAQDAKQEEP
                     KRSRKSAHRQTQLRETAVSTGESVEDRLEQAIAARRADASTNRREPIDDEVESMISAR
                     RMKRKDKSAGFCPKCGKPVLLSDRFCPSCGKALT"
     gene            complement(1075..1659)
                     /locus_tag="VFQ23_06085"
     CDS             complement(1075..1659)
                     /locus_tag="VFQ23_06085"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9906186.1"
                     /translation="MTELTNIAPRRGVPIWIQIIIWIFLVGLLVILGVQLNRTQEGTV
                     QPGSAIDDFTMELFEGYDYQGQSQIKLSDLRGKVVVINFWASWCKPCEQEAAALEAAW
                     REYEPTGEVVFIGADYVDTPTEASIYLKKFNITYPNGEDRDLQRGRPLSQYFRITGVP
                     ETYFVDKDGVLNFVHIGPLTTVEQIRERIDPLLE"
     gene            complement(1707..2225)
                     /locus_tag="VFQ23_06090"
     CDS             complement(1707..2225)
                     /locus_tag="VFQ23_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HET9906187.1"
                     /translation="MSKKFLRMTQYAMLAGLIAFVFASPAYAQDGTPTDDEVNKIAKQ
                     LYCPVCESTPLDVCPTEACRQWRDLIREMLTDGKSEAEIKQYFVEHYGPQVLNEPPNR
                     FLTYAVPIGAFLLGVLMLFQGFRMWKKPIPKAVIPITKGSADDRRNGRSDAKPAPDPY
                     IAMMEEELRKRN"
     gene            complement(2244..4304)
                     /locus_tag="VFQ23_06095"
     CDS             complement(2244..4304)
                     /locus_tag="VFQ23_06095"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET9906188.1"
                     /translation="MIANLGFGVLTVTFFVTLYSAFAALYGETKKIPGMVESARRAML
                     LTWPMLTLTSGILIYLLVNNHFEVQFVFEVTSLSMPTYLKVTAWWGGQAGSLLFWSWL
                     MSAFTSLVTLRKWDRDREFLPWVILVSCLTMAFFIGMNVFFENPFAQLYQTAAGVSAH
                     TFQPAGSTLVTPQDGRGLNPLLRHPGMIIHPPMLYLGFVSFVIPYAFAMAALITGRTD
                     DRWIRLTRRWSLWAWLFLSFGLVLGGRWAYDVLGWGGYWGWDPVEIAAFMPWLTGTAF
                     LHSVMIQEKRGMFKHWNMLLIILTYDLVIFGTFLTRSGVLSSVHAFAQSAIGPLFMGF
                     VTVTLVSSVALIFYRWDSLKSETEMTSLLSREALFLLNNLMFISILVVCFWGVIFPLI
                     SELFTGQKVTVGPPFYERATGPLFAALLFLMGIAPLSAWGHSTVKTLGRAVWKPTIAA
                     IVITIVLFATYTRNVYALIGFFLVAFVVLITFYEYWRGANARQKSQNENLFTAVWNLT
                     RRNRRRYGGYIIHLSMALMAIGILGIELFQMETQGTIQQGETLSLANYELRYQEIAQW
                     DDLPSGVNYRRAVLEVYEGNEYLGQLVPRIDYYYESQQNMTIPGQRSTMRDDLYVLLV
                     DWEPASPTGATFKIYVNPLVNWLWLGSLLFLIGIAIAAWPDREIESASVLARRPVPQT
                     SAAD"
     gene            complement(4308..4784)
                     /locus_tag="VFQ23_06100"
     CDS             complement(4308..4784)
                     /locus_tag="VFQ23_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET9906189.1"
                     /translation="MKINKFVIGGTLILGAVVFLIWTATASNLQFFMTVDELNAKRGE
                     VVDKNLRVSGAVIGDTIQYDAQSLTLSFEVAHVPADSPDIQTEGGLAEALHQAVLDPS
                     RNRMKVVYVGVKPDLLRNEAQAIMTGHLGADGVFYAEELLLKCPTRYEEAIPEQAG"
     gene            complement(4907..>5183)
                     /locus_tag="VFQ23_06105"
     CDS             complement(4907..>5183)
                     /locus_tag="VFQ23_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9906190.1"
                     /translation="DWLHEAVTDKEKMDKVRALQPIAEELGCTLSQLSLAWVLKNPNV
                     STAITGASRVEQLQENLKAADVAPKLTPEVLARINEAFGVKNEDDED"
BASE COUNT         1334 a         1331 c         1266 g         1252 t
ORIGIN      
        1 aacaattcga gcaattctct tcaaagagag ctttgcctct ttcaatttcc tcttgcgtgc
       61 tatggaaggt catcgcgtac gcaacaacat cccagcgttc ctgatcactg agacttgcaa
      121 aaggaggcat aaaccgttcg atattgccac gcgtgaccgt cgtataccat cgagctaatg
      181 aagccggtcg tgcgacctcc ggcaaaccaa acgccgggac cgtcacaccc tgcaattgga
      241 ttccctgcgg gccgtcgccc atgcccgttt caccgtgaca cggtgcacac ttctctacgt
      301 agattgctgc accggtttcg gtgtttggca tctctgcagg aaaaagggct aaagttggag
      361 caggggttgg tgagatgtaa ttcggaggag gtgtgatgtc ctcagccagg gtgaaattgc
      421 aagctgaaag aagaatggtg aaagtaataa tgagagtcga acgtaagttc atgaatattc
      481 cgttatgtca gtgccttccc acaggatgga caaaagcggt ctgatagcaa gacaggcttg
      541 ccacacttgg ggcagaagcc agcagattta tcttttcgtt tcatgcgccg cgcagagatc
      601 atcgactcaa cctcatcatc tataggctcg cgacggttag tggatgcgtc ggcacgtcgt
      661 gcggcaatcg cttgttcaag tcgatcctcg acgctctccc ctgtactcac agcggtctca
      721 cgaagttgtg tttgccgatg cgcagatttc ctggaacgtt ttggttcttc ctgttttgcg
      781 tcctgcgcgg gggttaggtt atcgattttt cgcaaaacgt ccgcaccttt ttgcaaaagg
      841 ttggtccgtt gggctggata atcctcatcg ggaattttcc ccaacttaaa atcaaagtcc
      901 agttcctgca gcgcattgat gattcgatct cgttctgcca tcaatgctga aagctcatgc
      961 tcctcgtgtg tgaccggccg ggcaaatctt tgtgccaggg gggcatacag gtacattgcc
     1021 accagcacaa ggattgccag agtaaagaaa agggctgcaa tgtccatgtc gaaattactc
     1081 tagcagaggg tcgatacgtt cgcgaatctg ctcaacggtc gttaacggac cgatgtgcac
     1141 aaagttcagc acaccgtctt tatcgacgaa ataggtttca ggaacaccag taatgcgaaa
     1201 atattgggaa agagggcgtc cccgttgcag gtcacggtct tcgccgttgg gataggtaat
     1261 attgaatttt ttgaggtaga tacttgcttc tgtgggcgta tctacatagt ctgcgccaat
     1321 gaaaacaact tcacccgtcg gctcatactc tctccaggca gcttccaacg cagccgcttc
     1381 ctgctcacag ggcttacacc aggaagccca aaagttaatg acgaccacct tacctcgcaa
     1441 atcagaaagt ttaatttgcg attgtccctg atagtcatag ccttcaaaca gctccatcgt
     1501 aaaatcgtcg attgcactgc caggctgcac tgttccttcc tgtgtgcggt ttaattgaac
     1561 gccaagaata acaagcaaac ccaccaggaa tatccagatg atgatttgta tccaaattgg
     1621 aacaccgcga cgcggggcaa tatttgtgag ttcagtcatg aaagttttcc tgccctatcc
     1681 taaattagga ggaggggtgg tggaacctaa tttctcttcc tcaattcctc ttccatcatt
     1741 gcaatgtaag gatctggcgc cggttttgca tcgcttcgac cgtttctgcg gtcgtccgcg
     1801 ctccctttgg taattggaat gactgctttt ggaatgggct ttttccacat tcggaagccc
     1861 tggaagagca tcaatacgcc gagaagaaag gccccaatcg gaaccgcata cgtgaggaaa
     1921 cgattgggtg gttcattcaa tacttgagga ccatagtgct caacaaaata ttgtttgatt
     1981 tctgcttcac tctttccgtc tgtaagcatt tctcgaatca ggtcacgcca ctgcctgcac
     2041 gcctctgttg ggcagacgtc cagcggtgtg ctttcgcaaa caggacaata caattgtttg
     2101 gcgatcttgt ttacttcatc gtccgtcgga gttccgtcct gggcataagc cggcgaagcg
     2161 aatacgaagg ctatcagccc tgccagcatc gcgtattgtg tcatgcgtaa aaacttttta
     2221 gacatgctga tttatttatt cactcaatct gccgcgctgg tttgcgggac gggtctgcgc
     2281 gccagaacag aggctgactc aatttcccgg tcaggccagg ctgcgatcgc aatcccaatg
     2341 aggaaaagta gacttcccag ccaaagccaa ttgaccaacg gattgacata gatcttgaag
     2401 gtcgccccgg tcggagaggc aggctcccag tccacgagca gaacgtagag gtcatctctc
     2461 attgtcgagc gttgacccgg aatagtcata ttttgttgtg attcataata atagtcaatg
     2521 cgggggacga gttgaccgag atattcatta ccttcataaa cttccaacac cgcccggcga
     2581 tagttgacgc cactgggcag atcatcccat tgagcgatct cctggtatcg taattcgtaa
     2641 tttgccagtg agagggtttc gccctgttga attgtgccct gcgtttccat ttggaagagc
     2701 tcgatgccga gaattccaat cgccatcaga gccatgctga gatggataat atagccgcca
     2761 taccgccgac ggttgcgccg cgtcagattc caaacggcgg tgaaaagatt ttcattttga
     2821 ctcttctgcc gtgcattggc cccacgccaa tattcataga aggtaatcag gacaacaaaa
     2881 gcaaccagga agaatccgat cagcgcatat acattacgag tataggtggc aaaaaggacg
     2941 atggtgatga cgatggctgc aatggttggt ttccagacag cgcgtcccag tgtcttcact
     3001 gttgaatgtc cccatgctga aagcggtgca attcccatga gaaacagtag agcggcaaat
     3061 agcggacccg ttgcccgctc gtagaacggc ggccccacag ttactttctg ccccgtaaag
     3121 agttcagaga tgagcggaaa gataactccc caaaagcata ccaccagaat actgatgaac
     3181 atcaggttat taagtagaaa taacgcctcg cgcgaaagca gggatgtcat ttctgtttcc
     3241 gatttcaaac tatcccagcg atagaagatc aacgctacag aggagaccag agtcaccgtc
     3301 acgaagccca taaataacgg accaattgca ctctgcgcaa acgcgtgaac cgacgacaag
     3361 acgcctgacc gcgtaagaaa cgttccaaag atgacgaggt catacgttag aataatgaga
     3421 agcatgttcc aatgtttgaa catgccgcgt ttctcctgga tcatgacgga gtgtaaaaat
     3481 gcggttccag tgagccaggg catgaaagca gcgatctcga ccggatccca gccccaatag
     3541 cctccccagc ccagcacatc gtacgcccag cgcccgccga gtactagtcc aaacgagagg
     3601 aataaccagg cccacaagct ccagcggcgg gtgagacgaa tccagcgatc gtccgttcga
     3661 ccggtgatca aggctgccat ggcgaacgcg tacgggatga caaatgaaac gaatcccaga
     3721 tacaacatcg gaggatgaat gatcatgccc ggatgtcgaa gcagtggatt caacccacgc
     3781 ccatcctgcg gagtcaccag agtgctcccg gccggttgga acgtgtgcgc tgaaacccct
     3841 gctgctgttt gatacagttg tgcaaacgga ttttcaaaga agacgttcat gccgatgaaa
     3901 aatgccattg tcagacacga aacgagaatg acccagggca ggaactcgcg gtcacggtcc
     3961 cactttcgga gcgtaacaag tgacgtgaac gcagacatta accacgacca aaatagtaaa
     4021 gaaccagcct gcccgcccca ccaggcagtt accttcaaat aggtgggcat cgaaaggctg
     4081 gtgacttcaa aaacaaattg aacttcgaaa tgattattga ccagaagata aatcagaatc
     4141 cctgaagtca gggtcagcat gggccaggtg agcagcatgg cgcggcgagc actttccacc
     4201 atgccaggga tctttttcgt ttctccatac agcgcggcaa acgcgctgta cagcgtaaca
     4261 aaaaatgtaa ctgttagaac tccaaatccc aaattggcaa tcattattta tcctgcctgc
     4321 tcaggtatcg cttcttcgta tctggtgggg catttaagca ggagctcttc ggcatagaaa
     4381 actccatctg ctcccagatg acccgtcata atggcctggg cctcgtttcg aagcagatcc
     4441 ggtttgacac ctacatagac gaccttcatg cggttgcgcg atggatccaa caccgcctga
     4501 tgtaacgcct cagccagtcc accttcggtt tggatatccg gcgaatcagc agggacgtgg
     4561 gccacttcga acgacagggt caggctttgg gcatcatatt gaatggtgtc cccgatcacc
     4621 gcgccagaga ctcgcaggtt tttatccact acttcccctc tcttcgcatt cagttcatcc
     4681 accgtcatga agaattgcag gttggaggct gttgcagtcc atattagaaa aacaaccgcg
     4741 cctaaaatca atgtgccgcc gatgacaaat ttattgatct tcatgctagc ctccacgcca
     4801 ggaattcata tgtgactaaa tgcactatga ttttacatga ggagattaat gtggactgag
     4861 aagtccacag acgaatcgga cctgatttat ctaaatacaa ttcaattcaa tcctcgtcgt
     4921 cctcattttt aacaccaaat gcttcattaa tgcgtgctaa cacttccgga gtaagtttcg
     4981 gcgcaacatc tgcagctttc agattttcct gcaattgttc cactcgactt gcacctgtaa
     5041 tcgcggtgct gacattcggg tttttgagaa cccaggcaag cgatagttgt gaaagagtgc
     5101 aaccgagttc ctcggcgatc ggctgtagcg ctcggacttt gtccattttt tctttgtcgg
     5161 tcactgcttc atgaagccag tcg
//
LOCUS       DASTJP010000200         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1263787, whole genome shotgun sequence.
ACCESSION   DASTJP010000200 DASTJP010000000
VERSION     DASTJP010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263787"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..538)
                     /locus_tag="VFQ23_06110"
     CDS             complement(74..538)
                     /locus_tag="VFQ23_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906191.1"
                     /translation="MDYLVFLDTRAGELEKILSGVKSMIVKEFVSAQSAEQPVRPDDS
                     LYFLRDNDECDVRVKATVVRVLACEKGQHEDLSHILKELQTRLQLTEEQYNCWSTRKQ
                     ALFIEFEAAQKIPEIHIASEKIIDRTGWMVLAENGPVTEEGVKNGSGNNFTG"
     gene            complement(677..1321)
                     /locus_tag="VFQ23_06115"
     CDS             complement(677..1321)
                     /locus_tag="VFQ23_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906192.1"
                     /translation="MNTSRTIHVMLVDDHNVVRSGLATFLRAYDDLDLVGEAKNGLEA
                     LNLCRRKKPDVILMDLMMPEMDGIAATRAILAECPDIKIIAMTSFEDEELVQGVLAAG
                     AISYLLKNVTSDELAKAIRDAFSGRSTLSPEAATALIHATRPKQQPSYDLTEREWQVL
                     NLVVQGQSNRQIADSMVISVATVKAHISNILSKLHVASRAEAISYAIKHRLVSL"
     gene            complement(1308..>3574)
                     /locus_tag="VFQ23_06120"
     CDS             complement(1308..>3574)
                     /locus_tag="VFQ23_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9906193.1"
                     /translation="IAWSFVPTVIILSAVAWFTFYSYQKVIGDLAIREDLAVLDTKIV
                     QAADGINNLINGPLRPIILNIDTKPDLPLEVRALNILDRAPNLDIFDGGIYFVDQHGI
                     IFKTQPEEPELIGQDWSDTFQFRYVRDNPPGAGPLTNIQAIGKNETGIVCVIWPMHNT
                     LSEFIGAGYYCLAIQPNEQDILYTTVNSLSLGQNVYILDGNQRIIYSPDPAELGQDLS
                     KETYIQQLLQSESRSVRFQKGMEDWVINYGTLVRAKNPSASWILVSEQSWDEVMQPSL
                     PYRRLLYLLLALGVIVPVLVTAYGVRHITEPIQKLIRAAEQLTAGEFKHKIEVKTGDE
                     IETLADQFNLMSAELDDSYSLLEKKVAERTHELATLNAIAARVSSSLDLNEIMADALE
                     RIMELTGMEHGIAYRVAGGEGGAANTGTDAEEAYLRVMALRGFPPNFADFGERVPLRR
                     SAAGVAGRRAEPVIWALEELPTVSALRERLATLGVKQVIAIPLMSKARMVGSLNLSTN
                     QSRTYPPEQVALLKTIGQQIGVAVENARLYEEAEQAAIAAERHRLARELHDAVTQTLF
                     SANLIADVLPRIWKRNPDEGFQNLEELRQLTRGALAEMRTLLLEMRPESLERADIKSL
                     LTQLADAFIGRVRVPLDLEITGDCELTHEVKLVLYRVAQEALNNIAKHSGARQVDVQL
                     DCQPGQLSLCIKDDGLGFEPDSLSPGHMGIAIMQERASSIGACLKIDSQPGQGTTVEL
                     DWRSGRKDGTDEHL"
BASE COUNT          809 a         1001 c          865 g          899 t
ORIGIN      
        1 cgcgagcggc tttgcatcca ctccgctccg tgttcctgct tgcagcctgg actcaacttc
       61 ctgcctaccg cttttagcct gtgaaattgt tcccggatcc atttttgact ccttcttccg
      121 taaccggacc gttttctgca aggaccatcc atcctgttcg atctatgatc ttttcggatg
      181 cgatatgaat ttctgggatc ttctgagcag cctcaaattc aatgaagaga gcttgttttc
      241 ttgttgacca gcagttgtac tgttcctccg ttaattggag ccgggtctgc agctctttca
      301 agatgtgcga caggtcttca tgctggccct tctcacaggc caagacccgg accacggtgg
      361 cttttacacg cacatcacac tcatcgttat ctctaaggaa atacaggctg tcgtcaggtc
      421 tcacaggctg ctcagcagat tgcgcagaaa cgaattcttt cacgatcatg cttttgacgc
      481 cgctaaggat tttctcaagc tcgcctgcgc gtgtgtcaag aaaaacaaga taatccattg
      541 aagtcattcc tcagatcagg gcaatgtact ctatgcacac tttaaaagaa aaactccccg
      601 ggcacatcgg tccgtcgggg agttctgagt tatccttttg attatatttt tcttatacta
      661 tagtaggagg ctgcatttat agtgaaacta atctatgctt gatcgcataa gagatggcct
      721 ctgccctgct tgcgacgtgc agcttcgaga ggatattgct gatgtgggct ttcaccgttg
      781 cgacgctgat gaccatggaa tccgcgatct gccggttgct ttgaccctgc accaccagat
      841 tcaagacttg ccactcgcgt tcggtcaagt cataagaagg ctgttgcttt gggcgggtgg
      901 catgaatcag cgccgtagcc gcttccgggg aaagcgtcga gcgacccgaa aacgcatctc
      961 ggattgcctt tgccagctca tccgaagtga cattcttgag caagtagctg atcgctcccg
     1021 cagccagcac accctgcacc agttcctcgt cttcgaagct ggtcatggcg atgatcttaa
     1081 tatccgggca ctcggccaga attgcccgcg ttgctgcgat gccatccatc tcgggcatca
     1141 tcagatccat caggatcaca tccggtttct ttctacggca aaggttgaga gcttccagac
     1201 catttttcgc ttcacccacc aggtccaagt catcgtaggc tcttagaaat gtggccaggc
     1261 cgcttcgcac cacattatga tcatccacca acatcacgtg gatcgtccta gaggtgttca
     1321 tcggttccat ccttccgtcc cgatctccag tcgagctcaa cagttgtccc ctggcccggc
     1381 tggctgtcaa tcttcagaca ggctccaatc gagctggccc gctcctgcat aatcgcaatc
     1441 cccatatgcc ctggggaaag tgaatcgggc tcaaaaccaa gtccatcatc tttgatacag
     1501 aggctaagtt gaccaggctg gcagtccagc tggacatcca cttgacgggc acctgaatgt
     1561 ttggcgatgt tatttagcgc ttcctgtgcc accctgtaca acacaagctt cacctcatga
     1621 gttagttcgc aatccccagt aatttctagg tctagcggaa cacggacgcg gccgataaag
     1681 gcatcagcca gctgggtgag aagcgatttg atatcggccc gctccagaga ttcgggcctc
     1741 atctccaaca gcagtgttct catctctgcc agcgcgccgc gcgtcaactg gcgcaattcc
     1801 tccaggttct gaaacccttc atccgggttg cgtttccaga tgcgggggag cacatcggca
     1861 atgagattgg cggaaaagag ggtctgggtt actgcatcat gcagctcacg agccagacgg
     1921 tggcgttctg ccgcaatggc agcctgttcc gcctcttcat acaaacgggc attttcaaca
     1981 gccacgccga tctgctgccc aatcgttttt aagagtgcga cctgctccgg cggataggtt
     2041 cggctctgat tcgtgctgag attgagcgat ccgaccattc gggctttaga catcagcgga
     2101 atcgcaataa cctgcttgac ccctaaagtc gccaatctct cccttagagc gcttaccgtg
     2161 ggcaactcct caagtgccca gatcaccggt tcagcgcggc gaccagcgac accggcggca
     2221 ctccgcctca aaggtaccct ttcgccgaaa tcggcaaagt tgggtggaaa cccgcgcaat
     2281 gccatcacgc ggagatatgc ctcctccgcg tctgttccgg tgttcgccgc gccaccttcg
     2341 ccgcccgcga ctcgataagc gatgccatgc tccatcccag tcagttccat aattcgctcc
     2401 agcgcgtccg ccatgatctc attgagatcg agcgagctgc tcacacgcgc agcgatggca
     2461 ttgagggtgg ctaattcatg ggtgcgctcg gcgacctttt tctctagcaa agaatacgaa
     2521 tcatccaatt ctgccgacat caagttgaac tggtcagcca gggtctcgat ttcgtccccc
     2581 gttttcacct caatcttgtg cttgaactca cctgctgtta gctgttcagc tgcccggatg
     2641 agcttctgga taggttcggt gatgtgcctg acgccgtaag cggtcactag caccggcacg
     2701 atcaccccca aggcgagcag aaggtatagg agccgccggt agggcagtga aggctgcatg
     2761 acctcatccc aactttgctc agagacgaga atccaggagg cagaggggtt cttagcacga
     2821 accaatgtac catagttaat cacccaatcc tccatcccct tttggaaccg gacactccta
     2881 ctctcacttt gaaggagctg ttgtatgtag gtttctttcg ataaatcttg gcccagttct
     2941 gctggatccg gtgaatagat aatgcgctgg tttccatcga ggatatagac attctggccg
     3001 agggacaaac tgtttacggt tgtgtacaga atgtcctgtt catttggctg aatggctaag
     3061 caataataac ctgctccaat aaattcactc agggtattgt gcatcggcca gatgacacaa
     3121 acaattcctg tctcgttttt cccgatggcc tgtatattcg taagcggacc cgcacctgga
     3181 ggattgtcgc gcacgtagcg aaactgaaat gtgtctgacc agtcctggcc aatcagctca
     3241 ggttcctcgg gttgtgtttt aaatattatt ccatgttggt caacaaaata aattccccca
     3301 tcaaaaatat ccagattcgg tgcacgatcc aggatattca gagcacgaac ttctaatggc
     3361 aaatcaggtt tcgtatcgat attaagaata atagggcgca gcggaccatt gatcagatta
     3421 ttgatcccgt cggcagcctg cactattttt gtgtcgagaa ccgccaaatc ctccctaatc
     3481 gccaggtctc cgatcacttt ttgataggaa tagaaagtga accaggcaac cgcagaaagg
     3541 atgatcacag tgggcacgaa agaccaggcg atga
//
LOCUS       DASTJP010000201         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_657724, whole genome shotgun sequence.
ACCESSION   DASTJP010000201 DASTJP010000000
VERSION     DASTJP010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657724"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFQ23_06125"
     CDS             <1..251
                     /locus_tag="VFQ23_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9906194.1"
                     /translation="CDGDNMHLIVDLSKVNYLSSIGIPMLINTAKSVSSRGGKLVLLN
                     PQQNVSDVLDIVGIQQIIPIYTDMEAAKASVTSDISSQ"
     gene            complement(231..740)
                     /locus_tag="VFQ23_06130"
     CDS             complement(231..740)
                     /locus_tag="VFQ23_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9906195.1"
                     /translation="MKILETERLILRQFSTDDADFILRLLNEPSFIQNIGDRGVRTLD
                     DARSYILRVPVASYEKNGFGLNLVTLRETGSSIGMCGLIKRDELEDVDIGYAFLPEYW
                     SRGYAVEAATAVKEYARTVIGLKRLVAITDPENQASIRVLEKIGLRFERMIRLSEDDI
                     ELKLLAADI"
     gene            complement(744..1214)
                     /locus_tag="VFQ23_06135"
     CDS             complement(744..1214)
                     /locus_tag="VFQ23_06135"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET9906196.1"
                     /translation="MNENDIEHLRTAIEIAGQARQHGNHPFGATLVDENNQVLLQAEN
                     TVVTGRDCTGHAETNLMRLATQHFSLEKLAACTLYTSTEPCAMCAGAIHWGNVRRVVY
                     ALGATELYDLVGPSPDHLMIPCRDVFARTERQVDVEGPALELAAEARAVHEGFW"
     gene            complement(1235..1843)
                     /locus_tag="VFQ23_06140"
     CDS             complement(1235..1843)
                     /locus_tag="VFQ23_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906197.1"
                     /translation="MAGIAALMFKYGILENLATVRRIPIGKFWNVTLLITPLTWLGPF
                     IFFSLHFILNLFNTQLVLNERLYQALIFTIAVEITTVLHAFGHILGGKIVHSAMDELL
                     ITTTRDVNLYHGDQSTVPGYIHLARSLGGPLFNLLMAGIFSVFAQIIPAGFGAALTAG
                     LVSTNLFFGLGSFLPIPSVDGEVIWREILRAFKGRRNKSHYE"
     gene            complement(1822..2634)
                     /locus_tag="VFQ23_06145"
     CDS             complement(1822..2634)
                     /locus_tag="VFQ23_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906198.1"
                     /translation="MNAPGSPISLPLRIWLAVEVLFGIGAVAAIGLSPAKTETNFAWP
                     IQPVVMAAVLGAFYASSALLFLLPFFAKRWEMIRVMILPTAIFCTVQLIATILHWDKF
                     SVGTTPFYVWFASYLLPPFIFVAAYVWHQRQAKSQGSDQPLPAWLYRFFLVCGILLSI
                     GALIIFIFPTVLIPIFPWQLTPLTARSLSGWIMLLGVILLSMIRENDKTRIRFGTPFL
                     ILLLPTLLIQLTRYADQVDWTNPTLWIGLLLAAVMGLCGLYLARGDWRESLR"
     gene            complement(2631..>2884)
                     /locus_tag="VFQ23_06150"
     CDS             complement(2631..>2884)
                     /locus_tag="VFQ23_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906199.1"
                     /translation="HIISGILAVVTLYRGGESGAFWFALGFGLFYLGLGVVGVISGQP
                     LGLGLQPFDHPIHLGLGVVGLIAAWLSFHRTTSMRKVAP"
BASE COUNT          816 a          749 c          688 g          631 t
ORIGIN      
        1 aatgtgatgg tgacaacatg cacttaattg tggatttatc caaagtgaac tacctttcct
       61 ctattggtat tccaatgctg atcaacacag ccaagtctgt ttccagccga ggtgggaaac
      121 tggtgctgct caacccgcag caaaatgtgt cagatgtgct ggatatcgtt ggtatccagc
      181 agatcattcc gatctataca gatatggagg ctgcaaaagc aagcgttacc tcagatatca
      241 gcagccaata gcttcaactc gatatcatcc tcagaaagcc gaatcattct ctcaaaccgc
      301 agcccgatct tttcaagcac gcggatcgac gcctggtttt cagggtcggt gattgctacc
      361 agccgtttca aaccgattac cgtgcgggca tattccttga cggctgttgc cgcctctaca
      421 gcatatcccc tggaccagta ctcgggcagg aacgcgtaac cgatatcgac atcttccaac
      481 tcatcacgtt ttatcagtcc gcacatccca atcgaagagc cggtttctct caaagtcacc
      541 agatttaagc caaatccatt tttctcatag ctggcaaccg gcactctcaa aatataagag
      601 cgtgcatcgt ccagtgttcg cacgcctcga tctccgatgt tttgaataaa ggatggctca
      661 ttcaataacc gcaggatgaa gtcagcatca tctgtggaaa actggcggag tatcaatctt
      721 tcggtttcga gaatcttcat aacctaccag aatccttcat gaaccgcgcg agcctctgct
      781 gccagttcca gcgccggacc ttcaacatcc acctgccgtt ctgtacgcgc gaacacgtcc
      841 cggcagggaa tcatcagatg atcgggcgaa ggaccgacaa gatcatataa ctcggtcgcg
      901 cccagggcat acacgacgcg tctcacattg ccccagtgga tggcaccggc acacatcgcg
      961 cagggttctg tacttgtata aagcgtacag gctgccaatt tctcaagcga gaagtgttgg
     1021 gttgcaagcc gcatcagatt ggtttccgcg tgacccgtgc aatcacggcc tgtgacaact
     1081 gtattttctg cctgcaatag gacttgattg ttttcatcca ccagagtcgc gccgaaagga
     1141 tgattgccat gctgccgcgc ttgccctgcg atttcaatcg ccgtacggag atgctcaata
     1201 tcgttttcgt tcatcaaata ccttcccgag aaatttattc atagtgagat ttatttcgtc
     1261 tgcctttgaa cgcgcgcagt atctcacgcc agatcacttc accatccact gaaggtatgg
     1321 gtaaaaaact tccaagcccg aagaaaagat tcgtagagac taatcccgct gtcagggctg
     1381 ccccaaagcc agcagggatt atttgtgcaa aaacagaaaa gatcccggcc atcagcaaat
     1441 taaaaagcgg accccctagc gagcgcgcaa gatggatgta tcccgggaca gtgctttgat
     1501 cgccatgata caaattgaca tctcgggttg tagtgatcag cagttcatcc atcgcgctgt
     1561 gcacgatctt tccaccaagg atatgcccga acgcatgaag gacagtcgta atttcaacag
     1621 cgatggtgaa aatcaatgcc tgatacaaac gctcgttgag aacaagttgc gtattgaaca
     1681 gattgagtat gaagtgaagg ctgaagaaaa taaaaggtcc aagccacgtg agcggtgtga
     1741 ttagaagagt gacattccaa aatttcccaa ttggaatgcg ccttaccgtt gcaaggtttt
     1801 caaggatgcc atacttgaac atcagcgcag cgattcccgc caatctcccc ttgccagata
     1861 aagcccgcag agtcccatca ccgccgcgag aagtaaacca atccataaag tgggattagt
     1921 ccaatcgacc tgatctgcat aacgcgtcag ctggatgaga agggtcggaa ggagcagtat
     1981 caggaacggg gtcccgaatc gaatgcgtgt cttatcgttc tcgcggatca tcgaaagcag
     2041 gatcaccccc aggagcatga tccaaccgga taacgaacgg gccgtcagcg gagtcagttg
     2101 ccaggggaag atgggaatca acaccgttgg aaagataaat atgataagcg caccgatcga
     2161 gaggagaatt ccacagacca aaaagaatct gtataaccag gccggtaatg gctgatctga
     2221 tccctgagat tttgcctggc gttgatgcca tacataagca gctacaaaaa tgaatggcgg
     2281 taacaaataa ctggcaaacc aaacataaaa gggagtagtg ccaacagaaa atttatccca
     2341 gtgaagaatg gtggctatca attgaacagt gcaaaagatg gcagttggca gaatcatgac
     2401 acgtatcatt tcccatcgtt ttgcaaaaaa cggaagtagg aataacaagg cagaagaggc
     2461 ataaaaagca ccgagcaccg cagccatcac cacaggctga atgggccagg cgaaattcgt
     2521 ctcagtcttt gccggggaga gtccaattgc ggcaacggca ccgatgccaa acagcacctc
     2581 taccgccagc cagattcgca ggggcaggga gattggtgat cctggtgcgt tcatggagcc
     2641 acctttctca ttgaagttgt gcgatggaag ctcagccagg cggcgatcaa cccgactacg
     2701 cctaacccta aatgaatcgg atgatcaaag ggctgcaatc ccaaccctaa cggttgaccg
     2761 ctgattactc ctaccactcc cagaccaaga tagaagagac caaaccccag cgcaaaccag
     2821 aatgcgccgc tttctccgcc gcggtacagg gtaacaacgg ccagaatgcc agagatgata
     2881 tgca
//
LOCUS       DASTJP010000202         4161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1379281, whole genome shotgun sequence.
ACCESSION   DASTJP010000202 DASTJP010000000
VERSION     DASTJP010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379281"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFQ23_06155"
     CDS             <1..350
                     /locus_tag="VFQ23_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906200.1"
                     /translation="FNRWIRGIKSIPTWAMLLLGQLATMLLIGLWHGITLNFILWGAW
                     HGLGLFLQNRWSDFLRMHFPTIGQNPRLQSVLQFSGILLTFHFVALGWVFFALSDPAL
                     SWQVLLALSGVRR"
     gene            449..1543
                     /locus_tag="VFQ23_06160"
     CDS             449..1543
                     /locus_tag="VFQ23_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906201.1"
                     /translation="MQNKPRLFAILSKAILLLLLFNFAFIALNNIPLGNFSLYNSIFP
                     GRERLPFGDHPQAYNINLFDLDAMFASHVLAGSPKASDEYRILLIGDSSVWGTLLRPE
                     ETLAGQLNSKAIPVCGKTAHVYNLGYPYISLMQELMILDEALQYQPDMVVWLITLESL
                     PIDKQFGSPLVSNNPERVRELIAKYGLNADPNDEALNHESKWDQTFVSQRRALANLLR
                     LQIYGAMWAATGLDQYYPETYERAQIDLEASDDFHKLKSLENALAWDVLDAGMRAVPN
                     TILVNEPILVSNGLNSDIRYNFFYPRWAFDDYRLRLNEFAAERNLPYLDLWDIAPIEE
                     FTNSGVHLTPAGEALLADKIAPAIQNTCNG"
     gene            1589..2512
                     /locus_tag="VFQ23_06165"
     CDS             1589..2512
                     /locus_tag="VFQ23_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906202.1"
                     /translation="MTACSAGQQSTPIVSQPTDSFVSSEDSAQVPPTEDSEPQLVAST
                     ATVSVPTATQKPPLAKDAWKNIPVVPIDVSERAREIYELGLSMGNDPTHFSIIGDCQN
                     VSSYFLSSFDTPGDFSLGSEYAYLQPTIEYYQGSYSRVSLAVKGGFNAAAVLSPLRSD
                     PKSCNPNESPLDCELRVWKPSIVIVSMETWWSEKPAEEYDKYMRRVLDRIIETGAVPI
                     IATKADNLEGDHSINATIAQIAYEYDIPLWNFWAALQHLSDHGLTGDGFHLTFARNFF
                     DDRVRMKSAWPWRNLTALQTLDAVRQGLTEE"
     gene            2671..3387
                     /locus_tag="VFQ23_06170"
     CDS             2671..3387
                     /locus_tag="VFQ23_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906203.1"
                     /translation="MRRSMPIKFNLLPFIESTAHKRGLIFGGIILVALLAFEVFNYSS
                     TSFALSDILGDLSFGPLRWATVLAIAFCGIDFAGIARIFTPERGRDEPAEVWYLFAAW
                     FLAASFNATLTWWGVSVAILKQSAEGGALLGPAMMTKAVPVFVAAMVLLIRVLLINTF
                     SIAGERIFSLAEERSAQHQNRSYYRPTTEPVRTSDSSFPRPAPKPAPAAASRPLYNEP
                     TYHPVGMSAQERSDNPATRR"
     gene            complement(3506..>4161)
                     /locus_tag="VFQ23_06175"
     CDS             complement(3506..>4161)
                     /locus_tag="VFQ23_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906204.1"
                     /translation="EFLHKTGLGRISFVEIQPDGTTSTSQPILEKPYHLSYPFIFEHG
                     GEYYMIPETAQQRAIDMYRCTRFPDQWAFHKTIMRDLRAVDATLIDYEGRWWMFVNIA
                     GEGGSTWDELHLFYADDPLTDQWIPHPMNPIISDVRFARPAGHIFRRDGQLIRPSQDS
                     SLRYGYALNLNRITKLTIDDYEEELLEKLEPPNEGDILAVHTHNFSGNLTVVDAMLK"
BASE COUNT          981 a         1111 c          993 g         1076 t
ORIGIN      
        1 ccttcaaccg gtggatacgc ggcattaaga gtatccccac ctgggctatg cttctactgg
       61 gtcagcttgc caccatgctg ctgatcgggt tgtggcacgg aatcacattg aactttatcc
      121 tatggggagc ctggcacgga cttggactct tcctgcaaaa ccgctggagc gatttccttc
      181 gtatgcactt ccctactatc ggacaaaacc cgcgccttca atctgtttta caattcagcg
      241 gcatattact cacatttcac ttcgtcgcgc tgggctgggt ctttttcgca ttgagcgatc
      301 cagcgctctc gtggcaggta ctgctcgcgc tgagcggagt gagacgctag gtaaacgtag
      361 tcgaagcgtg gttgttcata taactcggcg cttcgactgc aagcaggagc accacctgct
      421 ttccgctcag cgcgagaaat aataaattat gcaaaacaaa cctcgtcttt tcgcaattct
      481 gtctaaggca attttgttgc tcttgctgtt caactttgcg tttatcgccc taaacaatat
      541 tcctctcggt aatttttcct tatacaatag cattttcccc gggcgtgaac ggctgccatt
      601 tggcgatcat ccacaagcct ataacatcaa tctgttcgat ctggacgcca tgttcgcatc
      661 acatgtcctc gccgggagtc ccaaggcttc ggatgaatat cgcattttgc tcattggtga
      721 ctcatccgtt tggggtacac tgctcaggcc cgaggaaacg ctggcaggtc aattaaattc
      781 aaaggcaatt ccagtatgcg gtaaaacggc gcacgtttac aatctgggct acccctacat
      841 ctcgctgatg caagagctga tgattctgga tgaagcgttg cagtatcagc ccgacatggt
      901 cgtatggctc attacgctgg agtcgcttcc cattgataag caatttggtt caccgctggt
      961 gtcgaacaac cctgaacgcg ttcgagaact gattgcaaag tatggactga acgcagaccc
     1021 taatgacgag gctttgaatc acgagtcgaa atgggatcag acgttcgtga gtcagaggcg
     1081 agcgctggca aatctcctgc gcctgcagat ctatggagcg atgtgggccg ctacgggact
     1141 tgaccagtat tatcctgaga cgtacgaacg cgcccagatc gatctcgaag ccagtgatga
     1201 ttttcataaa ttaaaatctc ttgaaaacgc gctcgcctgg gatgtgctcg acgcagggat
     1261 gcgcgcggtg ccaaatacaa tccttgtcaa tgagccgatt ctggtgagta acggtctcaa
     1321 cagcgacatc cgctataatt tcttttaccc gcgctgggca tttgatgact atcgcctgcg
     1381 gctaaatgaa tttgcagcag aacgcaacct tccctacctg gatttgtggg atattgctcc
     1441 aatcgaggaa tttaccaaca gcggcgtcca tcttacaccg gcgggcgaag cattgcttgc
     1501 ggataaaatt gcccccgcga tccagaatac ttgtaacgga tagatcatga aatatagatt
     1561 attttttctc tttctaatat taactttaat gactgcttgt tcagcaggac agcaatccac
     1621 accgattgtg tctcagccga cagattcatt cgtttcgtct gaggattccg ctcaagtccc
     1681 gccgacagag gattctgaac ctcaactcgt tgcatctact gctaccgttt ccgttccaac
     1741 ggccacacaa aagccgccgc tcgcaaaaga tgcctggaaa aatatccccg tagtccccat
     1801 cgatgtaagc gagcgggccc gcgaaatcta cgagctgggg ttatctatgg gcaatgaccc
     1861 aacccatttc tcgatcatcg gcgattgtca gaacgtctct tcttattttc tttcgtcgtt
     1921 tgacacacct ggcgatttca gcctgggcag cgagtatgcc taccttcagc caacgattga
     1981 gtattatcag ggttcctact cgcgcgtcag cctggcggta aagggcggct tcaacgccgc
     2041 cgcggtactg tctcccttgc gttccgatcc aaagtcctgc aacccgaacg aatccccact
     2101 cgactgtgag ctgcgcgttt ggaagccatc cattgtcatc gtcagcatgg agacatggtg
     2161 gtctgaaaaa cctgcagagg aatatgataa atacatgcga cgggtactcg atcgcatcat
     2221 tgagaccggc gccgtgccga tcatcgcgac caaagctgat aacctggaag gcgaccacag
     2281 catcaacgcg acgatcgcac aaattgccta tgaatacgat attccattat ggaacttctg
     2341 ggcagcgctt caacatttat cggatcatgg tcttaccgga gatggcttcc atttgacctt
     2401 tgcccgtaat tttttcgatg atcgagtccg aatgaaaagt gcctggccgt ggcggaacct
     2461 caccgcctta caaaccctgg atgctgttcg ccagggactg actgaagaat agagatctga
     2521 taatggggaa acgataaaat cgcgcgttat ttgagcgaat tttctgttac atcttgacaa
     2581 attagaacaa gtgttctata tttggggcat cggttttctt gaatgtagtg tggccctcca
     2641 gataaatcat cagggccaca ggaggcttct atgagacgct cgatgccaat caaattcaac
     2701 ttacttccat ttattgaaag cacagctcac aaacgcggtt tgatcttcgg aggaatcatt
     2761 ctcgtggcgc tgctcgcctt cgaagttttt aactactcca gcacatcctt tgccctgagc
     2821 gacatccttg gtgatttatc cttcggacct ctgcgctggg caaccgttct tgcgatcgcg
     2881 ttttgcggca tcgattttgc aggaattgcc cgcatcttca cccccgaaag aggacgtgat
     2941 gaacccgcag aggtgtggta cctttttgca gcgtggttcc tcgcagccag cttcaatgca
     3001 acccttacct ggtggggagt ttcggttgcc attctcaagc aaagtgctga aggcggcgcc
     3061 ctgctcggcc ccgcgatgat gaccaaagct gtgcccgtct ttgttgctgc catggtcctg
     3121 ctcattcgcg tgctcctgat caacactttc tcgattgcgg gcgaacgtat tttctcactt
     3181 gcagaagaaa gatcagcaca gcaccagaac agatcgtatt accgtcctac tactgaacca
     3241 gtccgcacct cagattcgtc cttccctcgc cctgcgccca agcctgcacc tgccgcagct
     3301 tcgcggccgc tctataatga gccaacttat catcccgtcg gcatgagcgc tcaagagcga
     3361 agtgataacc ctgccacgcg gaggtgacca gtcccttaaa caaaacttcc accctctttc
     3421 gacctccttg ataacatcca acataacacc gacgtcatta cagtgacgtc ggtgttatgt
     3481 tttgtaatcg gggtgcaact gccggttatt tcagcatggc gtctacaacc gtcaaatttc
     3541 cggagaagtt atgggtatgc acagccagga tatccccttc attcggcggt tcgagttttt
     3601 ccagtagttc ttcctcatag tcatctatcg tcagttttgt gatgcggttc agatttaacg
     3661 cgtatccata tcgcagggaa gaatcctgtg aggggcgaat caactggccg tccctgcgaa
     3721 agatatgacc tgccggacgc gcgaagcgta catccgaaat gatcgggttc atcggatgag
     3781 gtatccattg atcggtgaga ggatcatcgg cgtaaaacag atgcagttca tcccacgtag
     3841 agccgccttc acccgcaata ttcacaaaca tccaccatcg accctcatag tcgatcaagg
     3901 ttgcatccac tgcccgcaaa tctcgcatga tggttttgtg aaaagcccat tgatccggga
     3961 agcgcgtaca acggtacata tcaattgcgc gttgctgtgc cgtttcaggg atcatgtaat
     4021 attcgccgcc gtgttcgaag atgaaaggat acgacagatg atatggtttt tccaagatcg
     4081 gttgtgaggt actggtagtc ccatccggct gaatttcgac aaaggatatg cgtccaagcc
     4141 cggtcttatg tagaaactct t
//
LOCUS       DASTJP010000203         5966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1161779, whole genome shotgun sequence.
ACCESSION   DASTJP010000203 DASTJP010000000
VERSION     DASTJP010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161779"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFQ23_06180"
     CDS             <1..274
                     /locus_tag="VFQ23_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9906205.1"
                     /translation="MFYRSMVYGGLINQVLPRVYSKQEFAQIKARILLILGGKEMIYN
                     NLQAAKRAARERIPGVQIEVISNAHHITALAQPEIVNEKILKFFAS"
     gene            384..2321
                     /locus_tag="VFQ23_06185"
     CDS             384..2321
                     /locus_tag="VFQ23_06185"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9906206.1"
                     /translation="MLKKILALCLTAILLSEAACASIPKPASTIASDEIADIIAEYRE
                     EIPQRMKQEHVPGLAIVVVDDQNILWAEGFGYTDWNRRIPVNADTLFSIQSMSKSFTA
                     TAAMFAAQDGLVDLDEPITTYLPDFHVNSIFEENPEQKITLRLLLSHTAGFVHEAPIG
                     GNNDLPGHNFEEHIISISDTWLRFPVGTRWGYSNLGIDLAGYILQVRSGMPFTQYVQE
                     KVLDPLGMTHSTLDIQQVRARSDKAMGHVPMPFRPPVEFLLIPSGGVWTTANDMARYL
                     QFHINIGKLGGVRLLNQDLAETMYEPPNPAALGAEYALGIGTANWQGTRRFQHGGGGF
                     GFNSNMVWYPELKLGAVVLSNADDVGLNMQLVDDVLGSIISADIPLYHSRASTLPSVE
                     PAYGFIQNGPALLTDTALSDLISSKALPLDEASKQRRQNFIGSYVQTKWGVPYIDAQV
                     EERNGIFVLTALGQNIMLIEIQPGLLFDLQGTSYEFPPTVSKSPNYATFKIDKGLLPL
                     WLTFYALCGVIFLSALLFGPIRGIRHKGTSTNEPPRTRWLAWISILSGLASLSSLVCL
                     GIIIFFPNLLYVPWPRPYSDLPWWQFALLSLPFASLVLAFVIALIAGLWIKAQTDGRK
                     IRLYYLGIALALLIFNLALVV"
     gene            complement(2330..3460)
                     /locus_tag="VFQ23_06190"
     CDS             complement(2330..3460)
                     /locus_tag="VFQ23_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9906207.1"
                     /translation="MNAYLQRHLGTKLFLSYLAIILVGVVVLILASQFVLPAAFDRHM
                     NGMMGNGGMPNGMMGQTNGSMSQLYIDFRASFNEALIYAAFAAMSAAILLSFLFSRNV
                     IAAVQSMSLATQRIAEGRYEERVQVNGEDELAQLAARFNQMAEKLSQVESMRRRLIGD
                     VSHELRTPLTAIKGSMEGLIDGVLPANDETYQQIHAEADRLNRLVDDLQELSRVESRA
                     YELDIRPLDVSALVQTVIKRLAAKAQAKHISLNLELDPDLPPALTNEDRAIQVLTNLT
                     GNALQYTHEGGNVTIFARHVNNEIQISVHDTGIGISAEHLSRIFDRFYRVDKSRSRQS
                     GGGSGIGLTIARALVEAHGGRIWAESAGEEQGSTFTFTLPVG"
     gene            complement(3457..4140)
                     /locus_tag="VFQ23_06195"
     CDS             complement(3457..4140)
                     /locus_tag="VFQ23_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906208.1"
                     /translation="MTKILVVDDEPSITKLVSAYLKPEGYEVFVASDGNAGLKAARTF
                     KPDLIILDVMLPGMDGIELLSRVRRDSEVYIIMLTAKTEEIDKIVGLSVGADDYVTKP
                     FSPRELVARVKAALRRLKTGANAGAGGRVLSFKHIHIDTGARTVHMGENPIELTAIEF
                     DLLKALAENLGRVLTREQLLEIVWGGEYFGEIRVVDVHLGHVRQKLGRDDLIVTVRGV
                     GYRFEDEVV"
     gene            complement(4216..5013)
                     /locus_tag="VFQ23_06200"
     CDS             complement(4216..5013)
                     /locus_tag="VFQ23_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906209.1"
                     /translation="MNKTVSTTLIVFSVLVLAVIIFFAGSMYARMNSFGPGNMMSGYD
                     WENGNAFGPSMMNGRGPNMMGGFGYNSNNFTPLTIDQTKTAAEKYLTNLNNSDLQIAE
                     VMIFDNNGYVMVEEASTGIGAFELLVDPVSQTAYPEHGPNMMWNLKYSSINHDNMMGN
                     HGGMMGWMMSGNGNMMQGWSSSTPLNVTAEMTVTPEQAIKYAQKFLDANNSGATATTG
                     PVQFYGYYTLDFEKDGKVAGMLSINGYSGQVFLHTWHGTFIEETEYE"
     gene            5342..>5966
                     /locus_tag="VFQ23_06205"
     CDS             5342..>5966
                     /locus_tag="VFQ23_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET9906210.1"
                     /translation="MSYSVLPFPPERQLTIDGGRISSRKHTVVALIEVDVTTARCFLR
                     EYKARTGESLSFTAFVIACLGQAVSENKAVHAYRDWRNRLILFDDVDVNISVEIEHEG
                     RKVVIPHFVRAANRRTVWDIHSEIRGVQIRPESTREFGVMWFVRLPRFVRDVFYWFVF
                     KNPHWLKASFCTVGLTAVGMFGNGSGWAIPYAVHTLEVALGGICEKPS"
BASE COUNT         1406 a         1530 c         1469 g         1561 t
ORIGIN      
        1 gatgttctat cgctcgatgg tttacggtgg gctgatcaat caggtcttac cccgcgttta
       61 ttcaaaacag gaatttgccc agatcaaggc gcgaatactg ctcattctgg gcggaaagga
      121 gatgatctac aacaatcttc aggctgccaa gcgcgccgcg cgggaacgta ttcccggcgt
      181 tcaaattgaa gtgatatcga atgcccatca tatcaccgcg cttgcccagc ctgagatcgt
      241 aaatgaaaag attctcaaat tctttgcatc ataaagcttg ttcatctggc caggaaggac
      301 tctccccgag atggggaagt ctattgtcga atgtgcaaat cccaattaaa gaaaatcatg
      361 tgatttcgta cgagcgatcc agtatgctaa aaaaaatcct tgcactttgt ttgaccgcca
      421 tcctcctttc tgaagcggct tgtgcttcaa ttccaaaacc ggcaagtacg attgcttccg
      481 atgagattgc agacatcatc gccgaatacc gtgaggaaat cccgcagcgg atgaaacagg
      541 aacatgttcc cgggctggca attgtggtcg tcgatgatca gaatatttta tgggcggagg
      601 gctttggtta taccgattgg aatcgacgaa tacccgttaa tgcagacacg ctattctcga
      661 tccagtctat gtccaaatca ttcacagcca cagccgcgat gtttgccgcc caggatggat
      721 tggttgacct ggatgaaccg atcaccacgt acctgcctga tttccatgtg aacagtattt
      781 ttgaggagaa tcccgagcag aagatcactc tgcgtttgct gctcagtcat actgctggat
      841 ttgtccacga agcaccgatc ggtggcaaca atgacctgcc aggtcacaat ttcgaagaac
      901 acattattag catctccgat acctggctaa gattcccggt cggaacgcgt tggggatatt
      961 ccaaccttgg gattgatctg gcaggctata ttctccaggt gcgttcgggg atgccattca
     1021 cacaatacgt gcaggaaaaa gtgttggatc ctcttggcat gacgcactct actttggata
     1081 ttcagcaggt gcgcgcccgg tcagataaag ctatggggca cgtcccaatg ccattccgcc
     1141 cgcctgtcga attcctgctc atcccatctg gtggggtatg gacgactgct aacgatatgg
     1201 cgcgttattt gcagttccac atcaatattg gcaaattggg tggagtacgc ctcttgaatc
     1261 aggatctggc cgaaaccatg tacgaaccgc cgaacccggc tgccctgggg gcagaatatg
     1321 ctttagggat tggaacagca aactggcagg gcacgcgtcg ctttcaacat ggtggtggtg
     1381 gttttggctt taacagcaat atggtttggt atccagaact taaattggga gctgttgtct
     1441 tgtccaacgc ggatgatgtc ggcctgaata tgcagcttgt tgatgatgtg ttgggcagca
     1501 tcatctctgc cgatatccca ctttatcatt caagggccag tacattgcct tccgtggaac
     1561 ctgcgtatgg tttcatccaa aatggtccgg ctctcctgac tgacaccgcc ctaagcgacc
     1621 tgatctccag caaagcattg ccgttggacg aagccagcaa acagcgccgc caaaacttca
     1681 tcggatctta cgtgcaaact aagtgggggg ttccgtacat tgatgcccaa gtcgaggaac
     1741 gtaatgggat atttgtcctc acagcgctgg gacagaacat catgctgatt gaaatacaac
     1801 cgggacttct tttcgatctt caaggtacct cttacgaatt ccctcccaca gttagcaaat
     1861 ctcccaatta cgcgacattc aagattgaca aagggctttt gccactctgg ttgactttct
     1921 atgccctctg tggggtaata ttcctgtctg cgttgctctt cggtcccata cgagggattc
     1981 gccataaagg tacgtccact aacgagcctc cgcggacccg atggttggct tggatctcga
     2041 tactttccgg gctggcttcg ctctcaagtt tggtctgcct tgggatcatc attttcttcc
     2101 ccaacctact atatgttcct tggccgcgtc cttattcgga tctaccttgg tggcagtttg
     2161 ccttactaag cctgccattt gcaagcctgg tgctggcatt tgtgatcgcg ctaatcgccg
     2221 ggctttggat taaagctcag accgacgggc gaaagattcg cctctattac ctcggcattg
     2281 ctcttgctct gctgatattc aatctggcgt tagtcgtttg agacagttat tatcctactg
     2341 gcagagtaaa agtaaaggta cttccctgtt cctcgcctgc cgactccgcc caaatgcgtc
     2401 caccatgcgc ctcgaccagc gcgcgggcaa ttgtcaagcc gatgccgctt ccccctccgc
     2461 tttggcgtga gcgcgatttg tccacgcggt agaagcggtc gaaaatgcgg gagagatgtt
     2521 cggcagaaat cccaattcct gtatcatgca cggagatttg aatctcattg ttgacatgtc
     2581 ttgcaaagat cgtgacgttg ccaccttcat gcgtgtattg caatgcgttt ccagtgaggt
     2641 tggttaatac ttggatggcg cggtcttcgt tagtgagggc aggcggaaga tcaggatcca
     2701 gttcgaggtt caacgagatg tgtttggctt gagctttcgc cgccagtcgt ttgatcacgg
     2761 tctgtacaag agcagaaacg tccagtggcc gaatatccaa ttcatacgcg cgggattcaa
     2821 cacggctgag ttcctgcaaa tcatccacca ggcgattgag gcgatccgcc tcggcgtgaa
     2881 tttgttgata ggtttcatca tttgcaggta acacaccatc tatcagccct tccatggaac
     2941 ctttgatggc ggtgagcggc gtacgtagtt catggctgac atcgccgatt aaacggcggc
     3001 gcatggactc gacctgactg agtttttccg ccatttgatt gaagcgcgcc gcaagttgtg
     3061 cgagttcatc ttcaccatta acctgtacac gttcttcata ccttccctcg gcaatacgct
     3121 gtgttgccag cgacatgctt tgtacagctg caatcacatt acggctgaaa agaaaactga
     3181 gcagaatcgc cgcactcatt gctgcaaacg ccgcatagat gagcgcctcg ttgaagctgg
     3241 cacggaaatc aatatagagt tgtgacattg aaccgttggt ttgccccatc attccgtttg
     3301 gcatgccgcc atttcccatc atgccattca tgtgccgatc aaatgctgcc ggcaagacaa
     3361 actgacttgc aagaattaac acaactacac caactagaat aatggcaaga tacgacagga
     3421 aaagttttgt gcccaaatgt cgttgcaagt atgcgttcat acgacttcat cctcaaagcg
     3481 atacccgaca ccgcgcacgg tgacgatcaa atcgtcgcgc cccaatttct gtcggacgtg
     3541 tccgagatgc acgtccacca cacggatttc gccgaagtac tctccacccc agactatttc
     3601 gagcaattgc tcgcgcgtca gcacacgtcc aagattttca gccagcgctt tcaacaggtc
     3661 aaattcaatg gccgtcaatt cgattggatt ttcgcccata tgaactgtgc gtgcgcccgt
     3721 gtcgatgtgg atatgcttga atgacaacac ccttcccccc gcacctgcgt tggctcccgt
     3781 tttcagacgc cgcagcgccg ccttgaccct tgccaccaac tcccgcggac tgaaaggctt
     3841 cgtgacataa tcgtccgcac cgacggagag acccacgatt ttatcgatct cttcagtctt
     3901 ggccgtcaac atgatgatat acacttcgga atcacgacgc actcgtgaga gcaattcgat
     3961 cccatccata cccgggagca taacatcgag aatgatcaaa tcgggtttga aggtgcgcgc
     4021 tgccttgaga cctgcatttc catcggatgc aacaaacacc tcataccctt cgggtttgag
     4081 ataagcactc accagcttgg taatggaggg ttcatcatca accaccagga tctttgtcat
     4141 gccgtaattc tacctgtaag caagccggtg acagtcactt cacctaacga aacgactgac
     4201 accttgaact aaagattact cgtattcagt ttcttcgatg aatgtgccat gccaggtgtg
     4261 caggaagacc tgtccactgt agccgttgat gctcaacata ccagccactt tgccgtcctt
     4321 ttcaaagtca agggtgtaat agccatagaa ctggactggg cctgtagtag cggttgcacc
     4381 tgaattattt gcatcgagaa atttttgggc gtacttgatt gcctgttctg gtgtaacagt
     4441 catttcggcg gtgacattta gtggagttga gctactccaa ccttgcatca tgttgccatt
     4501 gccactcatc atccatccca tcataccgcc gtggttgccc atcatattat catggttaat
     4561 gctgctgtat ttcaagttcc acatcatgtt gggaccgtgt tcaggatagg cggtttggga
     4621 aaccgggtca acgagcaact cgaaggcacc gatgcctgtg ctggcttcct cgaccattac
     4681 atagccgtta ttgtcgaaga tcatgacttc ggcgatttgt agatcagagt tgttgagatt
     4741 tgtcaggtat ttctcagcag ctgttttcgt ctggtcaatg gtcagaggag tgaaattgtt
     4801 gctgttgtag ccgaagccgc ccatcatgtt tggtccacgt ccgttcatca tcgaaggacc
     4861 gaaggcgtta ccgttctccc agtcgtaacc agacatcata ttcccgggtc cgaaggaatt
     4921 cattcgagca tacatgcttc ctgcaaaaaa gatgatcacc gccagtacga gaacggaaaa
     4981 gacgatcaga gttgtggaca cagttttgtt catggtttta tctcctattg gttgcaatgc
     5041 acagatctgt gcgctgtttt atgccaccag tataggattc ttacttgaaa aggctgtaaa
     5101 gcgatcataa agaaatgata aaggttattc ggctggaatc tccttgatcc cttgaggtat
     5161 tggttgttcc tcacacaaat cacaatccgc tgacagttac gctggagatt gttagcgagt
     5221 aaattccgga atttctcacg gcaaagtcac ctatctactc ggatagtgaa gactctccca
     5281 gcgatggggg aaggttgatc agttaggaaa tattattaaa gctgagatga atacaaactc
     5341 catgtcgtac tccgttctgc cgtttccgcc agagcgccag ttgactatcg acggtgggcg
     5401 gatctcatca cgcaaacata cagttgttgc tcttatcgaa gtggatgtga ccaccgcgcg
     5461 ttgctttctg cgcgagtata aggcgcgtac cggcgagtca ctatcattta ccgcgttcgt
     5521 gattgcctgc cttggtcagg ctgtgagtga aaataaggcg gtccatgcat atcgtgactg
     5581 gcgaaaccgc ctcattcttt ttgacgacgt ggatgtgaac ataagcgtcg agatcgagca
     5641 tgagggcaga aaagtagtca tcccgcactt cgtccgtgcc gcgaacagac gcacagtttg
     5701 ggatattcac tccgagatcc gcggcgttca aatccgcccc gaaagcacaa gggagttcgg
     5761 cgtgatgtgg tttgtgcgcc tgcctcggtt tgtccgtgat gttttctact ggttcgtctt
     5821 caaaaatcca cactggctga aggcaagttt ttgcaccgtc ggcctgactg cggtgggaat
     5881 gttcggcaac ggaagcggct gggcaatccc gtacgctgtc catactttgg aggtggcgct
     5941 cggcggcatt tgcgagaaac ccagcg
//
LOCUS       DASTJP010000204         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_220623, whole genome shotgun sequence.
ACCESSION   DASTJP010000204 DASTJP010000000
VERSION     DASTJP010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220623"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /gene="gpmI"
                     /locus_tag="VFQ23_06210"
     CDS             complement(<1..845)
                     /gene="gpmI"
                     /locus_tag="VFQ23_06210"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET9906211.1"
                     /translation="MSSAIEKRVMLIILDGWGHSPDPAVSAIDQAHTPVMDGLYEKYP
                     NAELTTHGLAVGLPAGQMGNSEVGHLNIGAGRVVDQELVRIAKAIQSRALHQNPTLLD
                     AIQDANRRNARIHLMGLLSDGGVHSHMHHLKALCDILSENARVPVFIHAFTDGRDVGP
                     HTGLGFVREINEYVALRHIRLASVVGRYYAMDRDKRWERTKIAYDALVHGTGTPTMDL
                     ISAVETSYAAGVTDEFILPIIQTGADGHPVGVIQAGDLIIFFNFRTDRPRQLISVLSQ
                     QAYPD"
     gene            1013..1387
                     /locus_tag="VFQ23_06215"
     CDS             1013..1387
                     /locus_tag="VFQ23_06215"
                     /inference="COORDINATES: protein motif:HMM:NF027349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HET9906212.1"
                     /translation="MKEGIVLFITCLIMSAVSAQSELTTALGSGNVAGISAHFGSKVE
                     LTIDGREAHISKLDAEVRLKEFYSTHQAKGFKAVHSGNSKSNESNYTIGELLTDKGNY
                     RVYIYFTSEGGQRIVAELRIEK"
     gene            complement(1517..>3765)
                     /locus_tag="VFQ23_06220"
     CDS             complement(1517..>3765)
                     /locus_tag="VFQ23_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906213.1"
                     /translation="LFYASCNPERKTNMSPNQSPPQAELIHRPKWRRRLPLIGLTFGT
                     LAFLWWYFSKLFKCPSCYLFVDSADGLKNYFTSVYYVMADSGLWFSGMNYPYGDHIVY
                     TDNQPFWSGMMKLASSLTAFDGHVVGTINMLMVFSILISVICIYLLLRRMQLPVWMAI
                     VGCLPIAFLSPQIARFNGHYSLAYTCYIPAFLCLMFWWCDKPQSIKRAVFLVLWIAVM
                     SYTHLYFLFISVCFLTVYGFVVWGANRFRWNRAFLNLIVVVAIAGAVVYLPVKLTDPI
                     DDRPTTVYGIQVYTSKAAGTFLPWFAPMDKILTDELKIKRPDGEAMSYVGFPGLILCI
                     FIVIFMFRRTWLNWRKPVLSNFSLSVTPMRLLATGIIVWIISTGWFYIIGGSVLLEVF
                     PVLGQFRSLGRLGWIFYYAYTLFVVWYLFDMLKRTGKPFVRTLIILLVGLLVSVWYWE
                     AWKYLDHQTKGIYHENKTFRGEEVYRNALQNIGVDPQEFQAILQVPLVSVGSERINIA
                     RGLWTLNRTYQCATETGLPIINSNMSRTSVSQSLELLQLISDPDIPKRRLELMNEKPL
                     LLLADHQQLIDAEKKLVQKGVHLTDVQDLSLYGLPLEAFHRVTEPAAEKQVLATNLFD
                     DIMNTEGFNGSGALHTMGKGETIMEFTSPDSDTLYFEYWAKITHDGVFFPAIAYQWYK
                     ADGSLGGEEVLSMHNYNPFNVVEQWIQSTMQFVTMPERARHVFIALERDGVFDECRIY
                     KPVDNTAN"
BASE COUNT         1037 a          940 c          854 g          934 t
ORIGIN      
        1 tagtccggat aagcctgctg ggacaatacc gagatgagct gacggggcct gtcggttctg
       61 aaattgaaga agatgatcag gtcgcctgcc tgaatgaccc cgacagggtg tccatctgcg
      121 ccggtttgta tgatagggag gataaactcg tccgtaacac ctgccgcata gcttgtttca
      181 actgcactga tcagatccat ggttggggtg cccgtgccat ggacaagcgc atcgtaggcg
      241 attttggtgc gctcccatcg cttgtcacga tccatcgcat agtacctgcc aacaacgctt
      301 gccaggcgaa tgtgccgcaa agcgacatac tcattgattt cacggacaaa gccaagacct
      361 gtatgcggtc cgacatcccg gccatcagta aaggcatgga tgaacaccgg taccctggcg
      421 ttttcggaaa gaatgtcaca gagtgccttc aggtgatgca tgtgagagtg cactccgcca
      481 tcggagagca aacccatgag gtggatccgg gcatttcgcc ggtttgcatc ctgaatcgcg
      541 tcaagcaaag ttggattttg atgtaaagcg cgggactgaa tcgctttcgc gatgcggacc
      601 aactcctgat caacaacacg tccggcacca atattcaggt gaccaacctc tgaattgccc
      661 atttgtccag ctggcaggcc tacagccagg ccatgcgtgg tgagctccgc attagggtac
      721 ttttcgtaca acccatccat cactggtgta tgggcttgat cgattgctga gaccgcggga
      781 tcggggctat gtccccagcc atccaggatg atcagcatga cgcgtttttc aatagcacta
      841 ctcattttat ggtctggcac gaaatatgat gcctttgatc gagttcagga tatgtttttt
      901 aatcaatagt cagggcaata ttgccaatct ttttcacatt atatcagcac gcccgatacg
      961 gaactaaacc acccctcacg cggttttacg cgagcattta actttgatta ccatgaaaga
     1021 aggcattgtc ttattcatta cctgtttgat tatgagtgct gtgagtgcac aatctgagct
     1081 cacgactgct ctcggcagcg gcaatgtggc tgggatttca gcgcattttg gctccaaggt
     1141 tgagctcact attgatggtc gtgaagcaca tatctcaaaa ctggacgctg aagtccggtt
     1201 gaaggagttc tattcaacac accaggctaa gggctttaag gctgtccatt ccggcaattc
     1261 gaaatccaat gaatccaact acacgatcgg tgagttgctg accgataaag gcaattaccg
     1321 ggtatatatc tatttcacga gtgaaggtgg ccaacggatt gtcgcggagc ttaggattga
     1381 aaagtgagtc tggtcctgga actgaccttg cggcgtccgc ctgaggcgga cccgaacgtt
     1441 tacctgcctt tggcatggcg tcgcctttaa cgcctaacat ccccaacaga cacatcccca
     1501 acagacagtt tataagtcaa ttggcagtgt tatcaacagg cttatagatt ctgcattcat
     1561 caaagacacc atcccgctct aacgcaatga aaacatgcct ggctctttcc ggcatcgtca
     1621 caaattgcat tgtcgactga atccattgtt cgacaacatt gaacggatta taattgtgca
     1681 tactgagcac ctcttctccg cccaggcttc catctgcttt gtaccactga tatgcaattg
     1741 ccgggaagaa cacaccatca tgcgtaatct ttgcccaata ttcgaagtac aatgtatctg
     1801 aatccgggct tgtaaattcc atgatcgttt ctccttttcc catggtgtgc aatgctccgg
     1861 atccattaaa cccttctgtg ttcatgatat cgtcaaaaag gttggtagcc agtacttgct
     1921 tttctgcagc cggttctgtc acccgatgaa atgcttccaa tggtaatcca tacaatgaca
     1981 agtcctgcac atcggtcaaa tgcacaccct tctgaaccaa ttttttttct gcatcaatga
     2041 gctgttgatg atctgcaagg agcagtaatg gcttttcatt catcagctct aatctccgct
     2101 tgggaatatc cggatcgctg atgagttgta acaactccag cgattgactt actgaggtcc
     2161 gcgacatatt gctgttaatt atgggcaacc cggtttcggt ggcacactga tatgtccggt
     2221 tcaatgtcca aaggccccgt gcgatattga tccgttctga acctacactg acaagcggta
     2281 cctgaagaat cgcctgaaat tcctgaggat caacaccgat attttgaagt gcgttgcgat
     2341 agacttcttc tccgcgaaac gttttgtttt catgataaat accttttgtc tgatgatcga
     2401 gatatttcca ggcttcccaa taccagaccg agactaagag gccaacgagc aagatgatca
     2461 gggtacgaac gaaaggctta cctgttctct taagcatatc aaacagatac cataccacaa
     2521 acagcgtata ggcataataa aatatccacc ccagccgacc aagactccga aactgaccaa
     2581 ggacgggaaa aacttcgagc agaacagaac caccgatgat ataaaaccag cctgtcgaaa
     2641 tgatccatac gatgattcct gtagctaaaa gccgcatcgg cgtaacgctc agcgagaagt
     2701 ttgagaggac tggctttctc caattcaacc atgtccttct gaacatgaat atcactatga
     2761 aaatgcaaag gatcagtccc gggaatccga catagctcat tgcctctcca tccggcctct
     2821 tgatctttaa ttcatcagtc aaaattttgt ccattggtgc aaaccagggc aggaatgttc
     2881 ctgccgcttt ggatgtataa acctgaatac cataaaccgt tgtgggtcga tcatcaatcg
     2941 gatctgtgag cttcaccggc aaatacacca cagctccggc aatggcaacc acgacaatga
     3001 gattcagaaa tgctcgattc caccgaaagc gatttgcacc ccaaaccaca aatccataga
     3061 cagtgagaaa gcaaactgaa atgaagagaa agtagagatg cgtataactc atgaccgcga
     3121 tccataacac cagaaagact gcgcgtttga tactctgcgg cttgtcacac caccaaaaca
     3181 tcaggcatag aaaggcaggg atgtaacacg tatatgcgag actgtaatga ccattgaagc
     3241 gggcaatctg tggactcagg aacgcgattg gaagacaacc aacgattgcc atccagactg
     3301 gcaactgcat tcgtctcaac aggagataaa tacaaatcac cgagatcaat atcgagaaga
     3361 ccatcagcat attgatcgta ccaacgacat gtccatcaaa tgcagtcagt gaacttgcca
     3421 acttcatcat gcccgaccag aatggctgat tgtctgtata cacgatatga tctccatatg
     3481 gatagttcat tccggaaaac catagtccgg agtccgccat cacataatat acagaagtga
     3541 agtagttttt caagccatct gcactgtcca caaacaagta acatgaagga cacttaaaca
     3601 gcttagagaa ataccaccat aagaatgcga gtgtgccaaa tgtcagtccg atcaggggca
     3661 ggcgcctgcg ccattttggg cgatgtatca actcagcctg aggcgggctt tgattcggag
     3721 acatattggt ctttctttcg gggttgcaag atgcgtaaaa caagg
//
LOCUS       DASTJP010000205        11372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_327251, whole genome shotgun sequence.
ACCESSION   DASTJP010000205 DASTJP010000000
VERSION     DASTJP010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327251"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..318)
                     /locus_tag="VFQ23_06225"
     CDS             complement(1..318)
                     /locus_tag="VFQ23_06225"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HET9906214.1"
                     /translation="MNRVGFQFKVRQDRLAEYKEHHNHVWPEMLAALRETGWHNYTLF
                     MREDGLIFGYFETEESLATAQAKMSAKEVNTRWQQFMSPFIDANARPDETFVELEEYF
                     HLD"
     gene            complement(352..3159)
                     /locus_tag="VFQ23_06230"
     CDS             complement(352..3159)
                     /locus_tag="VFQ23_06230"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HET9906215.1"
                     /translation="MTTLKVRVEHHRDALGIGKDRPRLSWIIETDEKEWRQAGYEIEL
                     YDAKGELRNQTGRVESDQSVLIDWPFEPLSSRERLSVRVRAWNKKDQLSAWSELLPIE
                     VGLLHAEDWTGHFITPDWEEDTSQPQPAPILRREFGLRAGIKAARLYITALGLYEAEI
                     NGQIVSDNLFSPGWTVYDQHLRYNTIDITGMLQEGSNVIGAILGDGWFRGRIGFGGGQ
                     RNIYGRHLGLLAQLEVHYEDGSNERIVTNDAESWRATTGPILVSDIYDGESYDARLEH
                     PGWSLPGFDDSNWKGVRKIEWNMEALEAPLGPPVRRMETIAPVSITQSPSGKTIVDFG
                     QNLVGRLSIRVNGPEGHTITLRHAEVLEHGELGTRPLRHAKARDCFTLRGGKIETWEP
                     RFTFHGFRYVEVNDWPGELNLEDLSAVVIHSDMERTGWFDCSDPLLNRLHENVVWGMR
                     GNFLDVPTDCPQRDERLGWTGDLEVFSPTASFLYDSSGFLQSWLKDLAIEQHKLGGSV
                     PHVVPNVLGNGAGAAAWADAATVVPWVLYQRFGDRQILENQFDSMRAWVDHVASLAGE
                     IYLWDKGFQFGDWLDPTAPPDKPGQARTHKAIVASAYFAYSARLVARAAEVLGRSEEY
                     IRYSTLADNARNAFAREYITPAGRLVNDAETAYALAIAFDLLPTAAQRQHAGDRLAEL
                     VRDSGYHIRTGFVGTPLICDALCSTGHYQTAYRLLLQQEVPSWLYSVTMGATTIWERW
                     DSMLPDGRINPGEMTSFNHYAFGAVADWMHRTIGGLTPAEPGYRRIEIRPRPGGGLTH
                     CRVTHVTPYGSAECAWRIENEKFDLYVHIPANTNASVTLPGDSETVEIGSGSWHWSIP
                     YQDPDARDAYTVDDLVGEILGESVARRAVMDVLDRVEAPGFLRMAIFDERNIPLRQAL
                     QMLPNHEEAVTKMNEALQNIK"
     gene            3454..5109
                     /locus_tag="VFQ23_06235"
     CDS             3454..5109
                     /locus_tag="VFQ23_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906216.1"
                     /translation="MTSYQFPQTVFPPTSRPNEVFLIASGDSRLPANQTCWPAQADME
                     QKIIAAFADESITIKRAHPYDEEMKHGFVWNQRMGMDVFKSIPSEAPLIVAVAVWQYS
                     YHVLAGLRDHRGPILTIANWSGQWPGLVGLLNLNGSLTKMGVTYSTIWSEDFKDEFFL
                     KGIRQWLQEGTIAHDTSHVRDIDLTRLPEEEAQLGVALAKRLKNNKAIFGIFDEGSMG
                     MYNAIIDDELLNPCGIYKERLSQSALLAEMRLVSDQEVQNARIWLDEKGMTFVVGTDE
                     ATELTDAQILEQLKMYIATVRMADTYGCDAIGIQYQLGLKDMTVASDLAEGLLNNVER
                     PPVYHKETGKALFAGKAVPHFNEVDEGAGVDALVTNLVWTSMELDPATTLHDVRWGRH
                     YKDEFIDDFVWVFEISGAVPPSHFADGYAGATSERQPPMYFPKGGGTIKGESKAGEIV
                     WSRVYIQHGTLHVDMGRGTVVALPSKEMQFRWASTTPQWPIMSAILHGVSRNQLMARH
                     KANHIQVVYAPSAEMADKALAAKAAMFAEMGLAVHVCGEVKVG"
     gene            5168..7039
                     /locus_tag="VFQ23_06240"
     CDS             5168..7039
                     /locus_tag="VFQ23_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9906217.1"
                     /translation="MSPTQGEYTNPLDEAGMQQSSLLKNENDFTFRDLNKNGKLDIFE
                     DPRQPTEARIDDLLSQMTLEEKAGMLFINGAVINGDGSIEEKPGAPGFGRSAIGQINN
                     QWMNHFNLWQIPGVPVVAAWHNTLQRFAERTRLGIPITIASDPRNHFNRNNVFSMAAA
                     DFSQWCEPLGFAAIGDIELVRQFADIVRQEYLAVGIRLALHPQIDLATEPRWPRISGG
                     FGEDAELTAKLGKAYIEGFQGKTLGAQSVACMTKHFPGGGPQKDGLDPHFEFQQGQIY
                     PGNNCDYHLIPFQSAFEAQTAAIMPYYGIPTDQTDENVAMSFNKTIITGLLREKYHYD
                     GVVCTDWGLINDAHMPDTIWPARAWGVEHLSAAERVLKAIEAGVDQFGGESCPEHVIE
                     LVKTRRLSEARLDQSVRRLLRLKFQLGLFDNPFVDESKISQIFGSPDSVRAGAASQRR
                     AMTLLKNEDHLLPVQGSPKVFVKNVDASVASRYADVVTSPQEADVAILRLETPWVPIE
                     TKNPMARSFHHGDLDFKEPARTEILSIIQTVPTIVVLYLDRPAVIPEISNATKALIAE
                     YGATDEAVLDVIFGKAKPEGTLPFELPSSMEAVRQQKADVPFDSENPLYNFGFGLSY"
     gene            7063..8163
                     /locus_tag="VFQ23_06245"
     CDS             7063..8163
                     /locus_tag="VFQ23_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906218.1"
                     /translation="MFNRKLIFLFYISVLLLTSCSGTSEIPVATATTATIATAPGVTA
                     MPTNVLRSDVWEELLKATPYPHEVPLPEPVLSPLDGTYAKVDPSPPQFWTCYRCADYR
                     PEGGPWRIQFDKGVMRIFYTITNWKSIASFTVDKNRLTIFNDPFCPHDIGEYEWATQN
                     GALSLKTINDSCAFDLRKENLTKQSWLACTADGQDQVGCSDITTPPSVEAPAQLPVTV
                     NVFGGDSHYFDTPPDVYAVANKDNVPSPDGIQIQFHDQTIGYGTHRVLWWDGDWIEAT
                     TDTSYTSIGVQLWGSGYLGWARVMFDDVEVWRGLTTSLGKEHAYFGGYIEITDFEPGT
                     HIIRVENLGFDYRPVKVWAFGFSNQKVQPTYP"
     gene            8211..8969
                     /locus_tag="VFQ23_06250"
     CDS             8211..8969
                     /locus_tag="VFQ23_06250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9906219.1"
                     /translation="MYTTDMYEGMLAETITMAGANGDVINAYLARPLGPGPFPAMVLA
                     HHMPGWDEWYREATYKFAHHGFITISPDLYFRAGHGTPEDVSAKVRADGGIPDDQVVG
                     DLAGAMKYVRALPYASGKVGVFGTCSGGRHAYLAACRAPGFDAIIDCWGGRVVMTPDQ
                     LNDKFPVSPLDYTRDLPCPILGIFGNDDQNPSPEQVDQHEAELKKHNKEYEFHRYDGA
                     GHGFFYYHSPAYRQQQAVDGWNKVFAFLEKHLNA"
     gene            9068..9340
                     /locus_tag="VFQ23_06255"
     CDS             9068..9340
                     /locus_tag="VFQ23_06255"
                     /inference="COORDINATES: protein motif:HMM:NF039404.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HET9906220.1"
                     /translation="MCTMIIEKVKIDGSGKGTSGWFNLEQANVSFDHPFNAPLEHALN
                     IDFVNESQGLSARVAVELSEQAARDLVKTILAVLDQAEATGNLATK"
     gene            9355..9849
                     /locus_tag="VFQ23_06260"
     CDS             9355..9849
                     /locus_tag="VFQ23_06260"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HET9906221.1"
                     /translation="MSWYLIVKFLHILAVTITMGGMFARQLVRGLAKKSDDVNKVKSL
                     TEVAIRIDRTMVIPWSNVIFLAGIVLAIMQKWPIFGFLQGASQNWLFASNILLIIMIL
                     LIPTVFIPHNKRVESLLQVALSEARVTPELRAALNDQRNILAHHAEEIIVLVITGLMV
                     LKPF"
     gene            complement(10066..10203)
                     /locus_tag="VFQ23_06265"
     CDS             complement(10066..10203)
                     /locus_tag="VFQ23_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906222.1"
                     /translation="MRILIWMLLMGDWKLRRATMAEWMASITVENAAWTPSPVVLMMK
                     P"
     gene            10255..10500
                     /locus_tag="VFQ23_06270"
     CDS             10255..10500
                     /locus_tag="VFQ23_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906223.1"
                     /translation="MDCIAYISIRNEEHESAATLLGAADALRMVIDSSITKVEQGKSA
                     KEILTWHSMLGEERFGYCRDKGGKLTMDEAIDLAIVD"
     gene            10765..>11372
                     /locus_tag="VFQ23_06275"
     CDS             10765..>11372
                     /locus_tag="VFQ23_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906224.1"
                     /translation="MSVILILSITLTNAHVVLADDGPTPEPTVSAEPTEEPIQPTLEP
                     TEPPIVATESPVVETPVATEAAPAEEAPVEEQEAPITELLTQAPENTDLVVLDENGQA
                     LPLTSQEALNTILETDPMWCPAGVLPGGVGCTTNFATINLLIADMIANTGTYDENGII
                     YFTSTPGSSFSLTSTTLNTDYTTLSNFNLTLQGGWNGLNGASA"
BASE COUNT         2938 a         2846 c         2840 g         2748 t
ORIGIN      
        1 tcaatccaaa tggaaatatt cttccagttc aacaaaggtt tcatcggggc gggcattggc
       61 gtcgatgaag ggagacatga attgctgcca gcgtgtgttg acttccttcg cggacatttt
      121 ggcctgggct gttgcaagac tttcttccgt ttcaaagtag ccaaagatca aaccatcctc
      181 acgcataaat aatgtgtaat tgtgccagcc cgtttcgcgt aaggcggcca acatttcagg
      241 ccaaacgtga ttgtggtgtt ccttgtattc tgcaagacga tcttggcgga ctttgaattg
      301 gaaaccaaca cggttcataa atgttttcct acgctatggt gataaaaagg tttattttat
      361 gttttgcaat gcctcattca tttttgtcac cgcctcctcg tggttcggaa gcatttgaag
      421 tgcctgccga agcggaatat ttcgttcgtc aaaaatggcc atgcgtaaaa agccgggagc
      481 ttcgacgcga tcgagcacat ccatcacggc ccgccgagca acagattcac caaggatctc
      541 acctaccagg tcatctacag tatacgcatc gcgcgcgtcc gggtcttgat agggaataga
      601 ccaatgccat gaaccagagc caatttctac ggtctcgcta tcgcctggca gagttacaga
      661 ggcatttgta ttcgcaggta tgtgcacgta cagatcgaac ttctcgtttt caattctcca
      721 cgcgcattct gctgacccat acggagttac gtgagttacg cgacagtgcg ttagtccgcc
      781 gccgggtcgt ggacgaattt cgatccgacg gtagccaggt tcagccggag tcagaccacc
      841 aatcgtccgg tgcatccaat ctgcaaccgc gccaaaggca taatggttga aggaggtcat
      901 ctcaccgggg ttgatccttc cgtcaggcag catgctgtcc cagcgttccc aaatcgtagt
      961 cgcgcccatc gtaaccgaat acagccagga gggcacttcc tgctgcaaca agagccggta
     1021 cgccgtctga taatgaccgg tgctgcatag cgcatcacag attagtggtg taccgacgaa
     1081 accggtacga atatgatatc cactatcgcg cacgagctcg gccagccggt caccggcatg
     1141 ctggcgctgt gcggccgtgg gaagcaagtc aaaggcgatg gcaagagcat aggcggtctc
     1201 agcatcgttc acgagtcgcc cggcaggcgt aatgtattcg cgcgcaaacg catttcgggc
     1261 gttatcagcc aatgttgagt aacgaatgta ttcctccgaa cgcccaagga cttcggcggc
     1321 acgagccacc agcctggctg aatatgcaaa gtaagcactt gccacaatcg ctttatgagt
     1381 tcgagcttgc ccgggtttat caggcggtgc tgtggggtcg agccaatcac cgaactggaa
     1441 tcctttatcc cacagatata tttcgcctgc taaggacgcg acatgatcca cccaggcgcg
     1501 catactatcg aattgatttt ccaaaatttg tctgtcgcca aatcgttgat acaaaaccca
     1561 tgggactaca gtcgcggcat ctgcccaggc tgcagcaccc gccccgtttc ccagcacatt
     1621 cggaacaaca tgcgggacac tgccgcccag cttgtgttgt tcgatcgcca gatccttaag
     1681 ccaggattgc aaaaagccgc tcgagtcata gaggaatgac gccgtaggcg agaatacctc
     1741 caggtcgcct gtccatccca ggcgctcatc gcgctgcggg caatcggtgg gtacatccag
     1801 aaagttgccg cgcattcccc agacgacatt ttcatggagg cggttgagca acgggtctga
     1861 acaatcaaac cacccggttc gttccatatc cgaatgaatc accactgcac ttaagtcttc
     1921 aagattcaat tcaccgggcc agtcattgac ctctacataa cgaaaaccat gaaacgtgaa
     1981 tctcggctcc catgtttcga tctttccgcc tcgcagggta aagcagtctc ttgctttggc
     2041 atgacgtagc gggcgcgtgc caagctcacc gtgctccagc acttcagcat gacgcaatgt
     2101 tatggtatgc ccttccggtc catttaccct tattgacaat ctgccgacta gattttgacc
     2161 aaaatcgacg atcgtttttc cggaaggaga ttgggtgatg gaaacgggcg cgatcgtttc
     2221 catccgacgg accggcggac ccaatggagc ttccagtgcc tccatattcc attcaatttt
     2281 acgaacacct ttccaatttg aatcatcgaa ccctggtaag gaccagcccg gatgttccaa
     2341 tcgggcatcg tacgactcgc catcatatat atcgctcacg aggatgggac cggtcgttgc
     2401 gcgccagctt tccgcgtcgt tggtaacgat tcgctcatta gaaccatcct cgtaatgaac
     2461 ttccagttga gctaacaaac caaggtgtcg tccataaata tttcgctgcc ctcccccaaa
     2521 accaattcgg ccgcggaacc aaccgtcgcc tagaatcgcg ccaatgacat tcgaaccttc
     2581 ctgaagcatt cctgtgatgt ctatggtgtt atagcgtagg tgttgatcat agaccgtcca
     2641 gccgggagaa aataggttgt cacttactat ctgtccgttg atctcggctt catataaccc
     2701 aagtgcggta atgtatagtc gggcagcttt gatacccgcg cgaagtccga actcgcggcg
     2761 cagtataggg gcaggttgag gctgactagt gtcttcttcc caatctggcg tgatgaaatg
     2821 accggtccaa tcctcggcgt gaagtaatcc aacttcaatc ggaagcaatt cactccaggc
     2881 tgaaagttga tcctttttat tccacgcgcg tacacggacg ctcaagcgtt cgcgcgacga
     2941 gagcggctca aacggccaat cgatcaacac cgactggtca gactcaactc gtccagtctg
     3001 attgcgtagc tcaccctttg catcatacag ctcgatctca taccctgcct gccgccattc
     3061 tttttcatcc gtttcgataa tccaggagag gcgcggacgg tctttaccga tgcctaacgc
     3121 atcgcgatga tgttcgaccc gaactttcaa tgtagtcata tatattttcc tcgtcaacga
     3181 aataagatgt tttctgacac tacaaggatc agccgagatt tcaacaagct tttcattaaa
     3241 cctgacagcg tgaactgcct ttcttgaaac gacccatctc cagaaaaaaa tagatcagtg
     3301 tacagttatg aggtgtgttt tttctcaagc aagaatacag gaaaaaattc attcttgcaa
     3361 gcgatggcaa ctattatcca atgtgcacga ttgacaatgt agcccgctat aatcgcagta
     3421 tgaatcgatg ccttcgtaga gaggaaacta gccatgacat cttaccaatt tccccaaacc
     3481 gtatttcccc caacatccag gccaaacgag gtttttctca tcgctagcgg agattcgcgt
     3541 ctgccggcca atcaaacctg ctggccagct caggcggata tggagcaaaa aattattgca
     3601 gcctttgctg acgaatccat aaccatcaaa cgagcacatc cttacgatga agaaatgaaa
     3661 catggcttcg tttggaatca gcgcatgggc atggatgttt tcaaatccat cccatccgag
     3721 gctcccctga tcgttgcggt ggcagtatgg caatatagtt atcatgtgct cgccgggttg
     3781 agagatcatc gcggtcccat attgaccatc gccaattgga gtggtcagtg gcccgggctg
     3841 gtcggtttgc tgaatttaaa tggatctttg acaaagatgg gcgtcaccta cagcacgatt
     3901 tggagtgagg atttcaagga tgaattcttc ctcaaaggga ttcgtcaatg gttgcaggaa
     3961 ggcacgattg cgcatgacac gagtcacgta agagacatcg atttaacgcg cctgcccgaa
     4021 gaggaagctc aactgggagt tgccttagca aaacggctaa agaataacaa agccattttt
     4081 ggaatctttg atgaaggtag catgggcatg tacaatgcca tcatcgatga tgaattgcta
     4141 aatccctgtg gcatctataa ggaacgtttg agccagtccg ctttgctggc agagatgcga
     4201 ctcgtttcag accaggaagt acagaatgct cgcatctggc ttgatgaaaa agggatgacg
     4261 tttgtagtag gcactgatga ggctacagaa ctaacggatg ctcaaatcct cgaacagctc
     4321 aagatgtata ttgccactgt aagaatggca gacacatatg gctgcgacgc catcggtatt
     4381 caatatcagc taggactcaa agatatgacc gtagcctcag atcttgccga aggcctactc
     4441 aataatgttg aacgacctcc cgtttaccac aaagaaacgg gaaaggcact tttcgctgga
     4501 aaagcagtgc cacacttcaa tgaagtcgat gaaggggccg gcgtcgatgc gcttgtcacc
     4561 aatcttgtgt ggacgtcaat ggagttggat ccagctacta cgctacacga tgtgcgctgg
     4621 ggcaggcatt ataaggatga gtttattgac gatttcgttt gggtatttga aatttccggc
     4681 gcggtgccgc cctctcattt tgcagatgga tatgcaggag caacaagtga acgacagcct
     4741 cccatgtatt ttccaaaagg tggtggcacg atcaaaggtg agagcaaggc tggtgaaatt
     4801 gtctggagcc gcgtttatat tcaacatggc actctgcacg tggatatggg tcgagggacg
     4861 gtagtagctc ttccatcaaa ggaaatgcaa ttccgctggg catccacaac tccgcaatgg
     4921 ccgatcatga gcgccatatt acatggcgtg agtcgtaatc agttaatggc tcgccataaa
     4981 gcaaatcata tccaagtggt gtatgcacca tccgcagaga tggctgataa agctctggcc
     5041 gcaaaggcag ctatgtttgc tgaaatgggg cttgccgtgc atgtatgtgg tgaagtgaag
     5101 gtgggctaat tttttcaatg gcgaaaggtt tctcaaatat aatgtttgca catcaaggag
     5161 aagaaccatg agccccacac aaggtgaata tacaaaccct ttggacgaag ctggaatgca
     5221 gcaatccagt ttgcttaaga atgaaaatga ctttacgttc cgcgacctca acaagaacgg
     5281 caaattggat attttcgaag atccgcgcca gcccaccgaa gctcgaatcg atgatctttt
     5341 aagtcagatg acgttagaag aaaaagcagg catgttattc atcaatggtg cggtgatcaa
     5401 cggggatggg tccattgagg agaagccggg cgcgccgggg ttcggccggt ccgcaattgg
     5461 ccagatcaac aaccaatgga tgaatcactt caatctctgg caaattccgg gagtgccggt
     5521 agttgctgcc tggcacaata ccctgcaaag atttgctgaa cggacgcggc ttggtattcc
     5581 tattactatc gcatcagatc cacggaatca cttcaaccgc aacaatgttt tttcaatggc
     5641 tgccgcagat ttttcgcaat ggtgcgaacc gttggggttc gctgccatcg gcgatattga
     5701 actcgtgcgg caatttgcgg atatcgtcag gcaggaatat ctggcagtgg gaatccgcct
     5761 cgccctccac cctcaaattg acctggccac cgagccacgc tggccccgca tcagcggtgg
     5821 gtttggtgag gatgctgagt taacagcaaa acttggcaaa gcatacattg aaggttttca
     5881 ggggaaaaca ctcggcgcgc aaagcgtagc ctgtatgacg aaacatttcc cgggcggcgg
     5941 tccacaaaag gatgggcttg atccgcattt cgaatttcag cagggacaaa tctaccctgg
     6001 aaataactgt gactaccacc tcatcccgtt ccagtctgcc ttcgaagcac agaccgcagc
     6061 gatcatgccc tattatggaa ttccaacaga tcaaaccgat gaaaacgtcg ctatgtcttt
     6121 caacaaaacg atcatcaccg gattgctacg agaaaaatat cattatgatg gtgtagtctg
     6181 caccgactgg ggcttgataa acgatgctca catgccagat accatctggc ccgcgcgcgc
     6241 ctggggcgtt gaacatctca gcgcggcaga aagagtacta aaagcgatcg aggccggcgt
     6301 ggatcagttt ggcggggaaa gttgcccgga acatgtgatt gaattggtaa aaacacggcg
     6361 actgtcagaa gcacggcttg accaatctgt gaggcgctta ctgcgcttga aattccagct
     6421 ggggcttttt gataatccgt ttgttgatga gtccaaaatt tcacagatat ttggatctcc
     6481 tgattcggtt cgagcgggtg cagcctcaca aagacgagcc atgacattgc tcaagaatga
     6541 ggatcacctc ctccccgtac aagggtcgcc gaaggtattc gtcaagaatg tagacgcgtc
     6601 tgtagcaagc cgctatgcgg acgtcgtcac gagccctcag gaggcggatg tcgctatttt
     6661 acgtctggaa acgccctggg tcccgatcga gacgaagaat cctatggcgc gtagctttca
     6721 tcacggcgac ctggatttca aagaaccagc gagaacggaa atcctgtcga taatccaaac
     6781 ggtacccacc attgttgttc tgtatctgga ccgccccgca gtcatcccgg aaatcagcaa
     6841 cgcgacaaag gcactcattg cggagtatgg tgccacggat gaagcagtgc tggatgtgat
     6901 ctttggcaaa gccaagcccg agggtacgct gccatttgag cttccctcct ccatggaggc
     6961 ggtgcgccaa caaaaggcag atgttccttt tgattctgaa aacccacttt ataatttcgg
     7021 atttgggtta tcctattgaa aagtttacta tagaggagat ctgtgttcaa tcgaaaattg
     7081 attttcttgt tctatatttc cgtgcttttg ttgacgtcct gttcaggcac atctgaaatt
     7141 cctgtagcga cggcaacaac cgctactatc gccactgcac caggtgtgac tgccatgcca
     7201 accaatgtgt tgcgtagtga tgtatgggaa gaattattga aagccacacc atatccccac
     7261 gaagttccgc tgcccgaacc ggtcttaagc ccattggatg ggacgtatgc aaaagtcgat
     7321 ccgtctccgc ctcaattctg gacgtgctac cgctgcgcag attatcgccc cgagggcggt
     7381 ccgtggcgaa tccagtttga taaaggcgtc atgaggatct tctacacaat tacaaattgg
     7441 aagagcatcg cgtcattcac ggtggataag aaccggttaa ccattttcaa cgatcccttt
     7501 tgtccgcatg acatcgggga atatgaatgg gcaacacaga acggagctct ttctttaaaa
     7561 acgatcaatg acagttgtgc gttcgacttg agaaaagaaa atttaacaaa gcaatcctgg
     7621 ctggcttgta cagcagatgg gcaagaccag gttggatgct cagatattac gaccccaccg
     7681 agcgtcgaag cacccgcaca acttccggtc actgtaaatg tgtttggcgg tgacagtcat
     7741 tattttgaca caccaccgga tgtatatgcc gttgcgaaca aagataacgt cccttcacca
     7801 gacgggatac aaatacaatt ccatgatcaa actattggat acggaacgca tcgtgtcctt
     7861 tggtgggatg gtgactggat cgaggctacc acagacacat cttacacttc gatcggtgtg
     7921 cagctctggg gctccgggta tctgggctgg gcgcgcgtca tgtttgatga tgtggaagtc
     7981 tggcgtggac tcacaacgtc cttgggcaaa gaacatgcct attttggagg ctacatcgag
     8041 atcactgatt ttgagccagg cacacacatc atccgcgtgg agaatttggg atttgattat
     8101 cgtccggtaa aagtttgggc gtttgggttc agtaaccaaa aggttcagcc aacgtaccca
     8161 taaaagtata atcaatcaaa atacagctca atacttaaag gagtctgctt atgtatacga
     8221 cggatatgta tgaagggatg cttgcggaaa ccatcacaat ggcaggcgcc aatggcgatg
     8281 tgatcaatgc ctatcttgca cgcccactgg ggcccggccc tttcccggcg atggtgctcg
     8341 cgcatcacat gccaggttgg gacgaatggt atcgcgaagc aacgtataag tttgcccatc
     8401 atggcttcat aacgatctct cccgatcttt atttccgcgc cgggcatggc acacccgaag
     8461 atgtctctgc gaaggttcgc gccgatggag gaatcccaga cgatcaggtg gtcggtgatc
     8521 tggcaggtgc aatgaaatac gtgcgcgccc tgccgtatgc cagcggaaag gttggggtat
     8581 tcggcacctg ctctggcggc agacatgcct atctcgccgc ctgccgcgcg cccggctttg
     8641 atgccattat cgattgctgg ggcggacgtg tcgtaatgac acccgatcaa ctaaacgata
     8701 aattcccggt ttcgccgctc gattacacca gagatctccc ctgcccgatc ttagggattt
     8761 tcggaaatga cgaccagaat ccttccccgg agcaggtcga tcagcacgaa gctgaactca
     8821 agaagcataa caaggagtac gaattccatc gatatgatgg cgctggacat ggattcttct
     8881 actatcacag cccggcctat cgtcagcaac aggctgttga tggatggaat aaggtcttcg
     8941 cctttttgga aaaacatctg aatgcctaat gtcattgcga ggagctgcga agcagcgaca
     9001 aagcaatctc gtggctataa agtattctac ttttcgttcg cagtcacatt tggaaaagga
     9061 aaaaaacatg tgcacaatga ttatcgaaaa agtaaaaatt gacggaagcg gaaagggcac
     9121 gagtggatgg ttcaatctgg agcaagcgaa tgtttctttt gaccatccct tcaacgcccc
     9181 actggagcat gccctcaata tcgattttgt gaacgagtct cagggactct ccgcccgcgt
     9241 ggcggtggag ttaagcgagc aggcggcacg agatctggtg aaaaccattc tggcggtgtt
     9301 agatcaggct gaagctacag gaaatcttgc aaccaagtag atggaagagc caacgtgagc
     9361 tggtacctga tcgtcaagtt cttgcacatc ctcgccgtga caatcaccat gggcggaatg
     9421 tttgcgcggc aattggttcg cggccttgca aagaaaagcg atgatgtgaa taaagtcaaa
     9481 tcgcttacgg aggtagcaat ccgaatcgac cgtacgatgg tcattccgtg gagcaacgtc
     9541 attttccttg ccggaattgt tttggccatc atgcaaaagt ggcccatctt cggcttttta
     9601 cagggcgctt cccagaattg gctttttgcc tcgaatatcc tactcatcat tatgattttg
     9661 ctcatcccca cagtatttat tcctcataac aagagagtgg aatcgctttt gcaagttgcg
     9721 ctttcagagg cgcgcgtcac tcctgaattg agagccgcgc tcaacgatca aaggaacatt
     9781 ctggcgcatc atgcagagga aatcatcgtc ctggtcatta cagggttaat ggtgcttaag
     9841 ccgttctgat tccacgaggt ataagatttg cacagggata accattatcg agccaaagta
     9901 taaggcttgg aatgactgac ccttcgatac agtttcccac aggcactgtc aaatttctgt
     9961 tcagtgacat tgagggccca actcaactct gggaaaaaca tccggaggaa atgaaggttg
    10021 ccatcgcgag tcatgattcg attcgagaga atccatcaaa tcaaatcatg gtttcatcat
    10081 caaaacgact ggcgatggag tccatgccgc attctcgacg gttatcgatg ccatccactc
    10141 agccattgtt gcacgacgca atttccaatc acccatcaat aacatccata tcaagattcg
    10201 catggggctg catttgaatt acattttgga tttcgcgcgg cggtcgcgca tgagttggat
    10261 tgtatcgctt acatttcgat cagaaacgag gagcacgaga gcgcggcaac tttgctgggt
    10321 gcagcagatg cattacgcat ggtgatcgac tcgtccataa caaaagtgga acagggcaaa
    10381 tctgcaaagg aaatcttaac gtggcactcc atgttgggcg aagagagatt tgggtattgc
    10441 cgggacaagg gaggaaagtt gactatggat gaggcgattg acctggccat tgtagattaa
    10501 cttctttttt tgaaggtacg cacactagga gtggggaatt acatataccg attttcaatt
    10561 caatctattc ccatcaaagc aatctacaag aggcaaatcc tgcctttaaa gctcgattta
    10621 ttgctggaac attgccagaa ccgacttttt tgtaacttgg actttacaga ttcgtcatgc
    10681 gtttgcaatt ttgaaataca ccgaacctat gaaggaaggt aagtgagcta catgcaaagc
    10741 aggaatcgtt tttttaattc gcttttgtct gttattctaa ttctcagtat cactctgacg
    10801 aacgcacatg ttgtcttggc agatgacggt cctacgccgg aaccgactgt cagcgctgaa
    10861 cccacggaag agccgataca acccacactt gagcctactg agccaccgat cgtggcgaca
    10921 gagtccccag ttgttgaaac acctgtcgca actgaggcag cgccagcgga ggaggctccc
    10981 gttgaagagc aggaagcgcc gattacagag ttgctcaccc aggcgcctga gaataccgac
    11041 ctggtcgttc tggatgagaa cggacaagca cttccactca cctctcagga agcgttgaat
    11101 acgattcttg aaacagatcc catgtggtgc ccggcagggg ttttgcccgg cggcgtcggt
    11161 tgtacaacca actttgctac cattaactta ttgatcgccg atatgatcgc gaacaccggc
    11221 acctatgatg aaaatggcat catctatttc acaagtaccc cggggtcatc tttctctctg
    11281 acgtccacaa ccctcaacac agactacacc acgctcagta actttaatct aacgctgcag
    11341 ggtgggtgga acggactcaa cggcgcaagc gc
//
LOCUS       DASTJP010000206         3314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_348099, whole genome shotgun sequence.
ACCESSION   DASTJP010000206 DASTJP010000000
VERSION     DASTJP010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348099"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFQ23_06280"
     CDS             <1..348
                     /locus_tag="VFQ23_06280"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HET9906225.1"
                     /translation="SVTALYEVKLYPEAYGNIATVFMRWQDPDTRQVVEISQDFNSGD
                     LERDFYYADPYFQRSVIVAEYAEILKKSYWAESTSLSHVFHEATRISELLPRDDVMAE
                     FMDLIRTASYLTD"
     gene            complement(410..2134)
                     /locus_tag="VFQ23_06285"
     CDS             complement(410..2134)
                     /locus_tag="VFQ23_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9906226.1"
                     /translation="MKTRLFTLFIVLGLVLSACGGTPAATSEPSSPATEPAAATEPPV
                     SESDGGTVTFVISEEPAGLNRYLADAAIVRQVSDATIIGLTEPNENGEYQPRLAADLP
                     AISEDRTTVTWTLREGLKWSDGEPLTSDDIVYTWEVISNPDSGAFNATSGFDLIESIE
                     TPDDLTAVVKYSEPYVDYLGQFSAGILPRHATGAPEEMATWEWNMTPVGAGPFVVTAW
                     QSGESITMEANPNYYEEGKPYLDRLIFRIAPEPAAQTALMISGEGQVHLWPSEDKADY
                     DPLMEGKAEQVLVPGLWNMAIDFNLSKPGDDDPGASAPHPILGDLRVRQAISHAIDYD
                     GLINDVLNAQVASSTSPFAYGWYKCEIPRMYSFDAEKAKQLLTEAGWVEGSDGIRVAQ
                     GAMHAEDGTRLSLELQGYTGYEPLERVEQFIVENLKAVGVEANIVNYDYSIIFGSYAD
                     GSPRKIGDYDMLIYDRSYGIEPHGTVVIDYLSTNIPTADNPDGDNHYRWVNPDADQAI
                     ETAGSTFDQSVRREAYCELGKLIQEDAVQNYIFLWQDGYGFSTDLTGYVVSTWGSMTW
                     DIQNWKYK"
     gene            complement(2223..3149)
                     /locus_tag="VFQ23_06290"
     CDS             complement(2223..3149)
                     /locus_tag="VFQ23_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906227.1"
                     /translation="MSETTLSIPEPIFSASLRPESLSALIWRRFRRHRLALISAIILV
                     FMILLVASAPLTGYTPTEQNTKNEFAKPSTTHLFGTDELGRDVFTRILHGGRISLAVG
                     IVSSLLSLLVGVIVGAVSGYYGGWIDNVLMRITDTFLIMPVVFVLILFSALLRESPAL
                     AGLRSSVWLVIFAVSAFAWMWPARIARGLCLVLRERDFVSASRALGASDARLIFQRIL
                     PNSIGPLLVSATLQMAFAIITESGLSYLGFGIQPPTPSWGNILESAQVHVYRAPWLAV
                     FPGLMIFITVMAINFIGDGLRDAFDPYVVHEK"
BASE COUNT          812 a          869 c          852 g          781 t
ORIGIN      
        1 tccgtgactg ccctgtatga ggtcaagttg tatcccgaag cctacggaaa tattgccaca
       61 gtcttcatgc gctggcagga tccagacact cgtcaggttg tggaaatctc gcaggatttt
      121 aacagcggtg atctcgagcg agatttttat tacgcggatc cctactttca gcgctcagtg
      181 attgtggctg aatatgccga gatcctgaag aaaagttatt gggcggagtc aacctcactt
      241 tcacacgtct ttcatgaagc gacgcgcatc agcgagttgc tcccgcggga tgatgtgatg
      301 gccgagttta tggatctgat tcgtacagcc agctatttga cagattgaca aaacaaaaat
      361 ggagcagagt taactctgct ccatttttga aaattattcc ttgcgcactt tatttatatt
      421 tccaattctg gatatcccag gtcatgcttc cccaggtgct aactacatag cccgtgaggt
      481 ctgtgctaaa gccatagcca tcctgccaga ggaagatgta attttggact gcgtcttcct
      541 ggatgagctt gcccaattcg cagtaagcct cacgacgcac agattgatcg aaggtgctgc
      601 ccgcggtttc gatagcctga tccgcatctg gattgaccca gcgataatga ttatcgccat
      661 caggattatc ggcagtggga atgtttgttg aaagatagtc aatcactacg gtgccgtgtg
      721 gctcaatgcc ataactgcga tcatagatca acatgtcgta gtcgccaatc ttgcgcggtg
      781 agccatcggc ataggaaccg aagataatcg agtaatcgta gttgacaata ttggcttcta
      841 caccaactgc tttgagattt tctacaataa actgctcgac gcgttcaagg ggctcgtagc
      901 cggtatatcc ctgcaattca agcgataagc gtgttccgtc ctctgcatgc atcgcaccct
      961 gtgccacccg gatgccatca gaaccttcca cccagcctgc ctccgtgagc aattgttttg
     1021 ccttttcggc atcgaagcta tacatgcggg ggatttcgca tttgtaccat ccataggcaa
     1081 acggacttgt ggaagacgct acctgtgcgt tcaaaacatc gttgatcagc ccgtcgtaat
     1141 cgatcgcgtg tgaaatcgcc tgccgaacgc gcaggtcgcc aaggatgggg tgaggggcag
     1201 atgcacccgg atcgtcatcg cccggcttgc ttaagttgaa gtcaattgcc atgttccaca
     1261 ggcccggcac caacacctgt tcggctttac cttccatgag cgggtcataa tctgccttgt
     1321 cttcgctggg ccagagatgt acctggcctt cacccgaaat cattaaggca gtttgcgcgg
     1381 caggctcagg agcaatacga aagatcagtc gatccaaata aggtttgcct tcttcgtagt
     1441 aattcggatt ggcttccatg gtgatactct caccgctttg ccacgctgtt acaacaaagg
     1501 gtcctgctcc aactggcgtc atgttccatt cccaggtcgc catctcttca ggcgcgcctg
     1561 tggcatggcg tggcaaaatt cctgcggaga actggccgag gtaatcgacg tagggctcgc
     1621 tgtatttcac cacggcagtg aggtcatcgg gagtttcaat gctctctatc aagtcgaagc
     1681 cagaggtcgc gttgaaggcg ccgctatcag gattcgagat tacttcccat gtgtatacga
     1741 tgtcatcgga ggtaagtggt tcaccatctg accatttcaa tccttcgcgc aaggtccatg
     1801 tcacggtggt gcggtcttcc gaaatcgcag gtaggtctgc tgcaagacgt ggttgatatt
     1861 ccccattttc attgggctcg gtcaagccaa tgatggtcgc gtctgatact tgtctgacga
     1921 tcgcggcatc agccaggtat cggttcagac cggctggctc ctctgagatc acaaaggtga
     1981 ccgttcctcc gtcggactca ctgacgggcg gctccgtcgc cgcggccggt tcggtcgctg
     2041 gtgaggaggg ttcagatgtg gccgcaggtg tgcctccgca ggcactgagc accagcccca
     2101 gcacgatgaa caatgtgaac aaccttgttt tcattttctc ttctccttat taagtttcat
     2161 acataatccg ttggtcgagt agtcccacat tttgtcgcgg gacatatcga gaccaacgtg
     2221 ttttatttct catgaactac atacgggtca aaggcgtccc gcaaaccatc cccaatgaag
     2281 ttaatcgcca tcacagtgat gaagatcatc aaacccggga atacagccag ccacggcgcg
     2341 cggtatacat ggacctgtgc gctttctaaa atattccccc agcttggggt aggcggctgg
     2401 ataccaaagc ctaaataact tagaccggat tcggtgatga tcgcaaatgc catctgaagg
     2461 gtggcgctga ccaataaggg tccaatgcta ttcggcagaa ttcgctggaa gatcagacgc
     2521 gcgtcacttg cacccagtgc gcgtgaggcc gagacaaaat ccctttccct caacaccagg
     2581 cagagaccgc gcgcgatacg agcaggccac atccaagcaa aggcacttac ggcaaaaata
     2641 accaaccaga cgctgctccg caaccccgcc agggccggcg attcacgtag cagcgcgctg
     2701 aacaagatca acacaaagac caccggcatg atcaaaaatg tgtcggtaat tcgcataagc
     2761 acattgtcga tccagccgcc gtaatatcca gaaactgcac ctacgatcac tcccaccagc
     2821 agggaaagga gcgatgaaac gataccaacg gccagcgaaa ttcgccctcc atgaaggatg
     2881 cgcgtgaaca catcccgtcc cagctcgtcg gttccaaata gatgggtagt agaaggtttc
     2941 gcgaattcgt tttttgtatt ttgttcggtt ggtgtatatc cggtaagagg ggcagaggcg
     3001 acgagcagta tcatgaatac cagaatgatc gccgaaataa gcgcgaggcg atggcgtcga
     3061 aagcgacgcc agatcaacgc ggatagactt tccggtcgca gggaagcgga aaagatcggc
     3121 tcaggaatgg ataacgttgt ctcactcata gcgaatacgt gggtcaagga aaccgtacaa
     3181 aatatctgca atcaggttga agaacaggat gagtgcagcc tggatcatta ccgttgccag
     3241 aaggacgggg taatctctgc ctctggcagc atcccaaaaa agccggccca ttccgggcca
     3301 ggaaaagatc gtct
//
LOCUS       DASTJP010000207         7150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_217222, whole genome shotgun sequence.
ACCESSION   DASTJP010000207 DASTJP010000000
VERSION     DASTJP010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217222"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFQ23_06295"
     CDS             <1..526
                     /locus_tag="VFQ23_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906228.1"
                     /translation="LIPLFAIAVTAIFLIVVRRNSDNPAKAYLFSDEANEIFVSSIAV
                     DVDLNSGITMDGPTPVAIPEQINNIDFAVFNHTDEPIRFTNQGFDLIVFRYDDVARKW
                     ENLPLRYVPHSETTILPPRLETRYSDVNNSWAILEDETTAWGYKQIRIYVAGKGTMTN
                     KTYGAYLDVTIHLP"
     gene            588..1052
                     /locus_tag="VFQ23_06300"
     CDS             588..1052
                     /locus_tag="VFQ23_06300"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HET9906229.1"
                     /translation="MPNYHLAQINIARMLAPIDDPIMAEFVAQLAPVNALADSSPGFV
                     WRLQSENGDATSIKVYDDDMIIVNLTVWESIEALREFVFKGAHHGVLRDRKRWFEKFD
                     GPYTALWWVPAGHLPSTEEGKERLDHLRAHGDTPFAFSFRNVFPNPSAVING"
     gene            1084..2706
                     /locus_tag="VFQ23_06305"
     CDS             1084..2706
                     /locus_tag="VFQ23_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9906230.1"
                     /translation="MFPDPEATEKHNKQLARLVELSVTLNSTLDLDALLQLITTTATD
                     LLDCEAASILLYDERQSRLYFAAATGSDPRELAEIPVPLDRSLAGTIFRTNQSIILND
                     VTHDTRHFTGVSKQVGFRVRSLVGVPMLAKGRVIGVLEGINKREGEFSESDKAILSVT
                     ASHAATAINNARLFRDAERRARNLDAIRYINNAVLNNPELQITLPQILSHLRARLEVD
                     AADVLLLDPHLNALTFAAGLGFRTKALNQAVLRLGEGIAGRVALEKKIVNIPDIKLAG
                     EIFTRGPFIKEEEFVAYHAAPLIVKGNIKGVLEVFHRAPLGGDLDWMEFFQTLAEQTA
                     IAIDSATLFTDLQRSHQDLKLAYDATILGWAHALDLRDKETQGHTQRVTRMTVQLARA
                     FGLGEEEITHIQRGALLHDMGKIGVPDAVLHKPSQLTDEEWFQMRRHPQYAYEMLEEI
                     DYLRPALDIPYCHHEKWDGTGYPRGLKGEEIPLAARLFSVVDVWDALYYDRPYRKGWD
                     LNRTLEYIREQSGKHFDPSVVEVFLRVVQEIM"
     gene            2722..3135
                     /locus_tag="VFQ23_06310"
     CDS             2722..3135
                     /locus_tag="VFQ23_06310"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9906231.1"
                     /translation="MKTIIDPPNFTKTVGAYSQGLKVDIGDKTMVFVSGQLAMDSDGK
                     PVAPDDISVQTRYIFENIKTILSNAGAALEDVVKVQIFLTDISKFPQVSAVRNEYLEH
                     IRPVSTLLEISRTVREGCDIEIEVIAITANKEKSE"
     gene            3132..3836
                     /locus_tag="VFQ23_06315"
     CDS             3132..3836
                     /locus_tag="VFQ23_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9906232.1"
                     /translation="MTENKTAIVTAAGKGMGAGIAKELANSGYKLALMSPSGSAEALA
                     KQLDGVGLTGSVAEADDLKKLVDLTMERFGRIDAVVNHTGHPPKGPLLDIPDSDWHAG
                     LDMLLLNVIRMARLVTPVMIQQGGGAFVNISTAPLFEPEPSFPVSATVRAGLAVFTKL
                     YSDQYAAQGIRMNVVMPGFIDSLTVVEERRRRIPMGRYGTVDEIAKTVRFLLSSESSY
                     ITGQSFRVDGGLMRSI"
     gene            3971..5152
                     /locus_tag="VFQ23_06320"
     CDS             3971..5152
                     /locus_tag="VFQ23_06320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9906233.1"
                     /translation="MGHKTYDVILVGGGVMGCATAYYLLKLNPNLNVAILEMDPSYEK
                     ASTPLSDGNTRIQFNIKENIQISQYGLEILARFSEELAVDGEKPDAAFRQQGNLFVLD
                     DASREESYEGLLLQQSLGCDVRWLSPEDVQRYYPIYNLKDCVAGTFGPDDGTMSPLAV
                     LLGYKKKVIALGANFINAEVVEILKDQDQIAGVKVASGEVLKSNVVVNTAGAWAPKIA
                     KTVGIDLPINPTKRQVTVIETNVRPDGILPALFLPSGLYCIHEHAGIFIVGKSFPDDY
                     VGLDDFAWERGKFEKVIWPELVEYIPEFDRLKVMRGWAGLYEVNALDGNSILGEWPEL
                     KGFYLANGFSGHGFQQCHGVGRYIAELILGVSPTLDLAVFSPQRILDNNPVFESRRKI
                     I"
     gene            5166..5891
                     /locus_tag="VFQ23_06325"
     CDS             5166..5891
                     /locus_tag="VFQ23_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906234.1"
                     /translation="MHENVAYALIHSPLVGPFTWQSVHQLMSSRGLTAIIPNLRDDFA
                     STLPYWQQHADSVAHELSRIPKTVSIALIAHSGAGPLLPAIRQRLSHSICAYIFVDAG
                     IPRDALSRLDLMRLENPQRVEEFHKSLLLGEKYPRWNEVDLQSIIPERETRQTLVADL
                     RPRSLSYFSEPIPVYDGWPDAPCLYIKFSEAYNSYAEHAKQADWDVYELKAGHFHMLV
                     EPLEVTDLLVGSVRKLLDASRLR"
     gene            6206..>7150
                     /locus_tag="VFQ23_06330"
     CDS             6206..>7150
                     /locus_tag="VFQ23_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HET9906235.1"
                     /translation="MNTEEYIGLFDDLDDNEQVENFINKDRRRKRFPDRHFKYVRAAS
                     ADFLKGQDDSSRSFDFTYQAARFEEWWLLESLGDFYEHKWISDVLRRLKGGKEASVYL
                     CRPGAEVDTDLVAAKVYRPRSLRNLKNDGTYRTGRVDLDAEGNALVKEADVYAIQKRS
                     GYGEELRHQSWIAYEFQTLEMLYAAGADVPKPYVLEKNAILMGYIGDVDNAAPTLNSV
                     TLDRDEVKPLYERVIRNIDLLLSHERVHGDLSAYNILYWDGDITLIDFPQVVYPEANP
                     AAWSIFLRDVTRVCQYFASQGWRRDARKVAAELWTSHGH"
BASE COUNT         1796 a         1674 c         1866 g         1814 t
ORIGIN      
        1 cctaattcct ttgtttgcaa ttgcagtaac ggctatattt cttatagttg tgcgccgcaa
       61 cagtgacaat ccagctaagg catatctgtt ttctgatgaa gccaatgaaa tctttgtttc
      121 ctcaattgcg gtcgatgtag atctgaactc aggtataacc atggatggac cgacacccgt
      181 ggcgatcccc gaacaaatca ataatatcga ttttgctgtg ttcaatcata ccgatgagcc
      241 aatccgattt accaatcagg gatttgatct gatcgtattc agatatgatg atgttgccag
      301 gaaatgggaa aacctgcctt tgcggtatgt tcctcactct gaaaccacaa tattgcctcc
      361 tcgcctcgag actcgatatt ctgatgtcaa taattcttgg gctatccttg aggatgaaac
      421 tactgcctgg ggatacaaac aaatacgcat atatgtggca ggcaaaggaa ctatgacaaa
      481 caaaacctat ggcgcctatt tggatgtcac tattcatctg ccctgatgga tgaagtatga
      541 ttgttccgta gagaatcaac aaagctgtac ataatcaaaa ggaacctatg cccaactacc
      601 accttgccca aatcaacatc gcccggatgc tcgcgcccat tgacgacccg attatggctg
      661 aattcgtcgc gcaactggct ccagtcaacg cgttggcaga ctcaagcccc ggcttcgtct
      721 ggcggcttca atccgaaaat ggcgatgcga cgagcatcaa ggtttatgat gacgacatga
      781 tcatcgtcaa tctcacagtt tgggaaagta tagaagcgtt gcgagagttc gttttcaagg
      841 gagcgcatca tggtgttctg cgcgatcgca aacgttggtt cgagaagttc gatggtcctt
      901 acacagcatt gtggtgggtt ccggctggac atttgcccag caccgaggag ggtaaggagc
      961 ggctggatca tttgcgtgcc cacggagata ctccatttgc gttttcattt cgcaatgtct
     1021 ttccaaaccc ttcagcagta attaacggtt gaaattaatc cataaactca gtacacttga
     1081 aaaatgtttc ccgacccaga agcaactgaa aagcataata aacagcttgc ccgcctggtt
     1141 gaattaagtg tcaccctcaa ttccacgttg gacctggatg cactacttca actgatcacg
     1201 accaccgcaa ctgacttgtt ggattgtgaa gcagcctcga tattgttata tgatgaacgc
     1261 cagtcccgct tgtactttgc cgctgccacc ggctctgacc cgcgagagtt ggccgagatt
     1321 cctgtgccac ttgacaggag tttggcagga acgatctttc gaacaaacca atccattatt
     1381 ttgaatgatg ttacccatga tacgcgtcac tttacgggcg tgtccaagca ggtaggcttt
     1441 cgagtgaggt cgttggtggg cgttccgatg cttgccaaag gtcgggtgat cggcgtgctg
     1501 gaaggaatca ataagcgcga gggcgagttc tccgagtcag ataaggcgat tttatccgtc
     1561 acggcatcac atgcagctac agcgatcaat aacgcccggt tgttccgaga tgctgaacga
     1621 cgcgcgcgaa acctggacgc gatccgctat atcaataacg cggtacttaa caaccctgaa
     1681 ctccagatca cgcttccaca aatcctgtcc catcttcgcg ctcgcctgga agtagacgcg
     1741 gcggacgtgc tgttactcga tccgcatctt aacgcgctga cctttgctgc cgggctggga
     1801 ttccgcacca aagcgttgaa ccaggcggtt ttacggctcg gggaaggaat tgccggtcgg
     1861 gtcgcgcttg aaaagaaaat tgtcaatatc cctgatatca aactggccgg tgaaatattt
     1921 acacgtggac cttttataaa agaggaagaa ttcgtagcct atcacgccgc gccgttaatt
     1981 gtcaaaggga atatcaaggg tgttctcgaa gtattccatc gggctccgtt gggtggagat
     2041 ttggattgga tggaattttt ccaaacgctg gctgaacaaa cagccattgc aattgacagc
     2101 gcgacactct tcaccgacct gcaacgctcg catcaggact tgaagcttgc ctacgatgcc
     2161 acgattttgg gctgggcgca tgcccttgat ttgcgagaca aagaaacgca aggacacaca
     2221 caacgcgtga cccggatgac ggttcaactt gcacgagcct tcggtttggg cgaagaagaa
     2281 atcacccata ttcaacgcgg cgcgctgctt catgacatgg gcaaaatagg cgtgccggac
     2341 gcagttctac ataagcccag tcagctgact gacgaagagt ggtttcaaat gcggcgtcac
     2401 ccccaatatg cgtatgaaat gctggaggag atcgattatc tcaggccggc gttggatatc
     2461 ccttattgcc accatgagaa atgggatggg acagggtacc cgcgcgggtt aaaaggtgaa
     2521 gagattccgc tcgccgcgcg tctgttttct gtggtggacg tttgggatgc actttactac
     2581 gaccggccct accgcaaagg atgggatttg aatcgcaccc tcgaatacat tcgtgaacag
     2641 agtgggaagc acttcgaccc cagcgtcgta gaggtctttc tgcgggttgt tcaggaaatc
     2701 atgtagttaa ggaaaataac tttgaaaaca attattgatc cgcccaattt tacgaaaacc
     2761 gttggtgcgt actcacaggg tttgaaagtt gatatcggcg acaagaccat ggtctttgtc
     2821 tctggtcaac ttgcgatgga ctcagatggc aaaccggtcg cgccagatga tatttccgtc
     2881 cagacgcgtt atatcttcga gaacatcaag acgattctgt caaacgcggg tgctgctttg
     2941 gaagatgtag tgaaagtaca aatttttcta accgatattt caaagtttcc tcaagtatct
     3001 gcagtgcgaa atgaatatct ggagcatata cgacctgtaa gcactttgct tgagatcagc
     3061 cgtacagttc gagaaggctg tgacatagaa attgaagtta tcgccattac cgcgaataag
     3121 gagaagtctg aatgaccgaa aataagacgg ccatcgtcac cgccgcaggc aaggggatgg
     3181 gcgcaggcat tgcgaaggaa ctcgcgaact ctggatacaa gctggcgttg atgtcgccat
     3241 ctggcagtgc ggaagcgctg gcgaaacaac tggatggcgt gggattaacc ggctccgtgg
     3301 cagaagccga tgatttgaaa aagttagtcg atctgaccat ggagcgattc ggcaggatcg
     3361 atgccgtggt gaaccatacc ggtcatcctc cgaaaggtcc gttgctcgat atccctgatt
     3421 cagattggca cgctggattg gatatgttgt tattgaatgt gattcggatg gcgcggcttg
     3481 tgacgccggt catgatccag cagggcggtg gggcatttgt caatatatct accgctcctc
     3541 tttttgaacc tgaaccaagt ttccccgtgt ctgcgacggt gcgggccggg ctggcggttt
     3601 ttaccaagct ctacagcgat cagtatgccg cgcagggaat tcgaatgaac gttgttatgc
     3661 ccgggttcat agatagtctg acggtcgtcg aagaacgcag gcgacgaatt ccgatgggac
     3721 gttatggcac tgtcgatgaa atcgcgaaaa cggttcgctt cctgctctcg tctgaatcca
     3781 gctacattac gggacaaagc tttcgcgtcg acggcggcct gatgagatca atttaagttt
     3841 gtcactgcga gggtaatgcc cgaagcaatc gcctggcgcc gggcaactct tctttagaag
     3901 gttagatgag cttgctttgc cggttttcat tctcctcatg cgtataccca aacaatagga
     3961 ctctccataa atgggacaca aaacttacga tgtaatcctg gtgggcggcg gggtgatggg
     4021 ctgtgccact gcctattact tactaaagct taacccgaac ctgaatgttg ccattcttga
     4081 aatggatccg agctacgaaa aagcgtccac tcctttatca gatggaaaca cccggattca
     4141 atttaacatc aaagagaata ttcagatctc gcagtatggg cttgaaatcc tcgcacggtt
     4201 ttctgaagag ctggctgtcg atggcgaaaa accggacgcc gcctttcgcc aacaggggaa
     4261 cctctttgtc ctggatgacg caagtcgcga agaatcctat gaaggtttgc tgttgcagca
     4321 gagcctgggt tgtgacgtgc gatggttaag tccagaggat gtgcagcgtt attatccaat
     4381 atataacttg aaagattgtg tcgcagggac gtttgggcct gatgatggga caatgtcccc
     4441 gctggctgtt ttgctgggct ataaaaagaa agtgattgcc ctgggagcga attttatcaa
     4501 tgctgaggtg gttgaaattc tcaaggacca ggatcaaatt gcaggggtta aggtagcctc
     4561 aggagaagta ttaaagagca atgttgtggt gaatacggct ggagcatggg cgccaaagat
     4621 tgcaaaaacg gtcggcatcg atttgcctat caatccgacc aagcggcagg tcaccgtgat
     4681 cgaaacgaac gtgcgccctg atggaatatt gccggcgtta ttcttaccat ctggcctgta
     4741 ctgtattcat gagcatgcag ggatctttat tgttggcaaa tcctttcctg atgattatgt
     4801 ggggctcgac gactttgcat gggagcgagg caaatttgag aaggtaatat ggcccgagct
     4861 ggtggaatat attccggaat ttgatcggct caaagtcatg cgcggttggg ccgggctgta
     4921 tgaagtcaac gcactggatg ggaattctat tttgggcgaa tggcctgaat taaaaggctt
     4981 ttaccttgcc aacgggtttt ctggccatgg ctttcagcaa tgtcatgggg tggggcgtta
     5041 tattgcagaa ctaattctgg gcgtttctcc tacgttggat ctcgccgttt tctcgcccca
     5101 gcgcatttta gataacaatc cggtattcga gagccggcga aaaatcatct agcaggaaga
     5161 atctattgca tgagaacgtt gcctatgcct tgattcatag tccattggtc gggccgttta
     5221 catggcaatc ggtccatcag ctgatgagta gtcggggact cacagcgatc ataccgaatc
     5281 tgcgtgatga ttttgcttcg accttaccgt actggcagca acatgcagac tcagttgcgc
     5341 atgagctctc tcgtatccct aaaactgtat caattgcatt gatagctcat agcggagcag
     5401 gtcctttgct accggctatt cgccaaagat tgtctcattc aatttgtgca tatatctttg
     5461 tggatgccgg gattccgcgc gacgcattaa gcaggctgga tttgatgagg ctggaaaatc
     5521 cacaacgagt agaagaattc cataaatcgc ttttgctggg cgaaaaatat cccaggtgga
     5581 atgaagttga tttgcagagc atcattcccg agcgtgaaac gcgtcaaacc ttggtggcgg
     5641 atcttcgacc tcgttcactc tcatatttct cagagcccat cccagtctat gatggatggc
     5701 ccgacgcgcc atgtttgtat atcaagttta gtgaggcata taactcgtat gcggaacatg
     5761 caaaacaagc tgattgggat gtgtacgaat tgaaagcagg tcattttcat atgctggttg
     5821 agcctctcga ggtaactgat ttgctcgtag gaagcgtgcg aaagttactt gacgcctctc
     5881 gcctccggtg ataaactcgc cctatcttat tctctcttga aaggaggcac gacaacatga
     5941 aaaccagcac aatatccaaa acaatcatct tgagcgcgcc tcccagggac aaatcgtaac
     6001 aacgactttc atcgttttat gttgctatac atccgcaggc gaagctcacc ctgcggtttt
     6061 tcttttaagc cacagacctg acgggtcgcc gcggggaatc gataggctga aatccacgaa
     6121 tctatgattt gatcaagccg tctgtaacag aaacttgacc cgtcaggtct ttcgacaaac
     6181 aaggttaagc aacagaatga ataagatgaa taccgaagaa tatattggat tgttcgacga
     6241 cctggatgac aacgaacaag ttgaaaattt cataaataag gatcggcgcc gcaaacgatt
     6301 ccccgaccgg catttcaaat acgtgcgggc agccagcgcg gattttctca agggtcagga
     6361 tgattcgagc cggagtttcg atttcacgta tcaggcggct cgttttgaag agtggtggct
     6421 gctcgaatcg cttggtgatt tttatgagca caaatggatt tcggatgtgc ttcgacggct
     6481 aaagggtggg aaagaggcct ccgtctattt atgtcgtcct ggggcggagg tggacacaga
     6541 tcttgtcgct gcgaaagtgt atcgtccccg ctcgctgcga aacctcaaaa atgacggcac
     6601 gtatcggacg ggtcgagttg acctggatgc cgagggcaat gcccttgtga aggaagcgga
     6661 tgtttatgcg attcagaagc gttccggcta tggtgaggag cttcgccacc aatcctggat
     6721 tgcttacgag tttcagacgc ttgaaatgct ctacgcagcc ggagcagacg tccccaaacc
     6781 gtatgtgttg gaaaagaatg ccatcctgat gggctacatt ggggatgtag acaatgccgc
     6841 tccgactctc aactctgtca ctcttgaccg ggatgaggtg aaacctcttt atgagcgtgt
     6901 cattcggaac atcgacctgc ttctctctca tgagcgagta cacggcgatt tgtctgccta
     6961 taacatcctc tattgggatg gcgacattac actaattgat tttccacaag ttgtgtatcc
     7021 tgaggcaaac cctgccgcat ggagcatctt cctgcgtgat gtgacgcgtg tctgtcaata
     7081 ttttgcctcg cagggctggc ggcgcgatgc gcgtaaagtc gcggcagagc tatggacgtc
     7141 tcatgggcat
//
LOCUS       DASTJP010000208         4904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_4403,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000208 DASTJP010000000
VERSION     DASTJP010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4403"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFQ23_06335"
     CDS             <1..536
                     /locus_tag="VFQ23_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HET9906236.1"
                     /translation="LGIVKARDLLVRSLSGEPIRLKDLLKPAFFIPETMFASRALEIF
                     KEKGTEILLVIDEFGGLQGILTINDILEEIVGQIELEEPQATQRQDGSWLLDGMLEVD
                     EFKEIFNVHGLPHEDEYETLSGFVMMSLGRVPQAADEFEWHGLRFEVIDMDGRRVDKV
                     LVTTLPHRPVTAETSGK"
     gene            576..1325
                     /locus_tag="VFQ23_06340"
     CDS             576..1325
                     /locus_tag="VFQ23_06340"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9906237.1"
                     /translation="MPRNFIRAVIFDLGGTLMYERESWHAVNSKADEALTKYLLGQGL
                     EINLSTFPIEFRRRLDEYFKQREKDLLETTYSSVLRDVLASKGYGNLSETILRNALDR
                     LFAVTQTNWALEEDALPTLKKLEESGYKLGLISNAGDDKDVQQLARGFRIAKYFDFIL
                     SSAACSYRKPHPRIFELALANWYLLPSEAVMVGDNLSADIRGAKNVGIYGIWISRRAG
                     QRTEEELQIKPDVSLASLSELPAVLDFLQVK"
     gene            1357..2253
                     /locus_tag="VFQ23_06345"
     CDS             1357..2253
                     /locus_tag="VFQ23_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9906238.1"
                     /translation="MTQNKSALAYLALGVGVLALSFSAMFVRWADAPGPITAFYRIFF
                     SIFLLLPFLLPRLKKNPDVRSRSVLFPILAGIFTACDLALWTSSLSYTTAANATLLGN
                     TAPLWVALGTWLILKQTLTTAFWRGLSITLIGAALIMGTDFILHPRFGVGDLMATFTG
                     FFYGGYYLITEKSRTHFDSVVHIWFVGIGASLALFIINGILQNPFVGYDRITWLVFLS
                     SAFVSQLVGYMALAYALGHLPASIVAPTMVLQPVVTAILAIPFLGEIPTIWQGIGGAI
                     ALVGIYVVNRSHQTALQETPNG"
     gene            2363..2767
                     /locus_tag="VFQ23_06350"
     CDS             2363..2767
                     /locus_tag="VFQ23_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9906239.1"
                     /translation="MDIQVHTENGRVPVTVLHVHGNIDSSTYHEFEAKAEELIKEGTR
                     YILVDLSHSGFISSAGFRALNHIFKKLREVHPDTNLSDDEMKKGISAGTYKSPHLKLL
                     KMSPETRTAFEMSGFDMYIDTFTDMQAAVASF"
     gene            2907..4313
                     /locus_tag="VFQ23_06355"
     CDS             2907..4313
                     /locus_tag="VFQ23_06355"
                     /inference="COORDINATES: protein motif:HMM:NF009881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9906240.1"
                     /translation="MPDLFDHAMQERMKTEAPLAARMRPRTLDEYIGQEHIVGEGKLL
                     RRAIEADRLFSSIILWGPPGTGKTTLAQVIANTTKSHFITISAILAGKADLRVIIQEA
                     LERRRLHNERTILFVDEVHRWNKAQQDALLPHVEAGTVTLIGATTENPYFEVIKALIS
                     RSRVFQLRNLNQIETGILIDRALTDKERGYGDKRVLLHDDARSHLVDVASGDARNALN
                     AIELAVESTPPDKDGVIQITLDVAQESIQRRAVLYDKDGDAHYDTISAFIKSVRGSDP
                     DAALYWLAKMIYAGEDPRFIIRRLIILAGEDIGLADPMGLVVVSSAAQAFDFIGLPEG
                     IYPIVEATLYLATAPKSNSAGAYFKAMKKIEDEGQVSVPRHLMDGNRDAAAMGHGKNY
                     VYPHDHEGHFTPQQYLPKRLLGTYFYAPSQEGYEAQVTARLEMWRDAQRKALGITKVE
                     NIPAMTEEQIQDMKRKIK"
     gene            4358..>4904
                     /locus_tag="VFQ23_06360"
     CDS             4358..>4904
                     /locus_tag="VFQ23_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9906241.1"
                     /translation="MNLSHIEATVSEYDVAIIGAGPAGSAAGYFLATGGLRVVLLDKF
                     DFPRDKICGDGLTPRAIKILAKIGILPQLEKTAFHCSELKIRYSDELTFNLGLENLEN
                     LPNYILTMPRLQLDHALLQHAINAGAKFFPKAKVESISRDFDKHIHLEIESGRRIECA
                     LAIVATGANTRLLQNLGLSKKT"
BASE COUNT         1255 a         1152 c         1222 g         1275 t
ORIGIN      
        1 ttctaggaat cgttaaagcc cgggatttat tggtgaggag cttgagtggt gagccgattc
       61 ggttaaaaga tttgcttaaa ccagccttct tcatccctga gacgatgttt gcctcgcgcg
      121 cgctcgagat cttcaaggaa aaaggcacgg agatcctact tgtcattgat gagttcggtg
      181 gcctgcaggg aatcttgacc atcaacgata ttctcgagga aattgtgggt cagattgagt
      241 tggaagaacc tcaagctacc caaagacaag atgggtcatg gctgcttgat ggaatgctgg
      301 aagtggatga attcaaagag atattcaatg tacacggcct tccacacgag gatgaatacg
      361 aaaccctcag cgggtttgtg atgatgtcgc ttggccgcgt cccccaggca gccgatgaat
      421 ttgaatggca tggattgaga tttgaagtga ttgatatgga tggtcgccgc gtggacaagg
      481 tccttgttac gacgttaccc catcggccgg tcactgcaga gacgagtgga aaataatcca
      541 actatctaag acctgttgaa ctcaacgatg ctcttatgcc acgcaacttc attcgcgcag
      601 taatctttga ccttggtgga acgcttatgt atgagcgtga atcctggcat gccgtcaatt
      661 ccaaggcaga tgaagcgctc acaaaatatt tgctcggcca gggattggag attaatttat
      721 cgacctttcc catcgaattt cgccgcaggc tggacgaata cttcaagcag cgggaaaagg
      781 atttactgga aacgacatat tcgtcggttt tgcgtgatgt attagccagc aaggggtatg
      841 ggaatttatc tgaaacgatt cttcgcaacg cgctcgatcg cctttttgcg gtcacgcaaa
      901 cgaactgggc attggaagaa gatgccttgc ccacgctcaa gaaattggaa gaaagcggat
      961 ataaactcgg attgatatcc aacgccggtg atgataagga tgtacagcag ctggcgcgag
     1021 gcttccgcat cgcgaaatat tttgatttta ttctcagttc ggctgcctgc agttatcgaa
     1081 aacctcatcc tcgtattttt gaacttgcac ttgctaactg gtacctcctg ccctcggagg
     1141 cagtgatggt gggtgataat ctttctgcgg acattcgcgg cgcaaaaaat gttggaattt
     1201 atggcatctg gatcagtcgc cgcgctggcc aaagaacgga agaagagctt caaatcaagc
     1261 cggacgtgag cttggcctcg ttatctgaac ttcccgctgt actcgatttt cttcaagtta
     1321 aatagaattt attacctttc aacggatcag acatcaatga ctcaaaataa atccgcgctt
     1381 gcctatctcg cgttaggcgt gggagtgctg gcgctcagtt tttctgccat gttcgtacga
     1441 tgggcagatg cacccggacc aatcaccgcc ttttatcgga ttttcttttc gatttttttg
     1501 ttgctcccgt ttcttttgcc acgcttaaag aagaatccgg atgtgcgatc gcggtcagtt
     1561 ttgtttccaa tcctggcagg gatttttaca gcctgcgatt tggccttgtg gacatcctct
     1621 ttatcttata cgacggcggc aaatgccaca ctgctgggaa acactgcacc actctgggtg
     1681 gctttaggta catggctgat cctgaaacag actttaacaa cggctttctg gcgcggtctt
     1741 agtatcactt tgatcggcgc ggcgctgatc atgggtaccg actttatcct gcatccgcgc
     1801 ttcggagtcg gtgacctgat ggctaccttt actggattct tctatggagg ttactacctc
     1861 attactgaga aaagccgtac acactttgac tcagtggttc acatttggtt tgtcggcatc
     1921 ggagcaagcc ttgccttatt tatcatcaac ggtatcctgc aaaatccatt tgtcggttac
     1981 gaccgaataa catggctggt gtttctgtcc agtgcctttg tgtcacaact ggttgggtat
     2041 atggcgcttg cgtatgcgct tggccacctc ccggcatcca ttgttgctcc gacaatggtc
     2101 ttacaaccag tggtcacagc aatcctggct atcccctttt taggagaaat tcccacgatt
     2161 tggcagggca ttggaggcgc aattgcgtta gtagggattt atgtggtcaa tcgatctcat
     2221 caaactgcat tgcaggaaac tcccaatggt taagaacata tccgtttctg gcttctggct
     2281 tatggagatg aattacttct cgtgcacggt agcgtaaagt ataatacgag cagattggtt
     2341 attattcgca gaggagaatt atatggatat ccaggtacat accgaaaacg gacgagttcc
     2401 ggtaaccgtg ttgcatgttc acggaaatat cgattcatca acctatcacg agtttgaagc
     2461 aaaggcagag gaattgatta aagagggcac tcgttatatt cttgtggatc tttcacattc
     2521 cggctttatt agtagtgcag gctttcgagc tctgaatcat atttttaaga agcttcgaga
     2581 agtacatccg gataccaacc tttccgacga tgaaatgaaa aaggggatca gcgctggcac
     2641 gtacaaatct cctcatttga agttgctgaa gatgtccccg gaaaccagga ccgcatttga
     2701 aatgagcggt ttcgacatgt acattgatac gttcacagat atgcaggcag ctgtcgcgtc
     2761 gttctaaccc catattgggt tcgcattaag ggcgccattc ccgcgccctt tttttattct
     2821 atttttccac tcgcgctgag cggaggcttc gaacgctctt agaggagccg cagacgaagc
     2881 actgagggaa atgctataat ctcgttatgc ccgacttatt cgatcacgcc atgcaagagc
     2941 ggatgaaaac cgaagcgccg cttgccgcgc gcatgcgccc acgcacactg gatgaatata
     3001 tcggtcagga gcatattgtc ggggaaggca aactcctgcg gcgcgctatt gaagcagaca
     3061 ggctgttctc ttccataatc ttgtggggtc ctcctggcac tggcaaaact accctcgcac
     3121 aagtgatcgc caataccaca aagagtcatt tcattacgat ctcagccatt ctcgccggca
     3181 aagcagattt gcgtgtcatt attcaggaag ccctcgagag aaggcgcctc cacaacgaac
     3241 gcacgatcct ttttgtggat gaagtccacc gctggaacaa agcccagcag gatgccctgc
     3301 ttccgcatgt cgaggccggg actgtcacat tgatcggtgc gacaacggaa aatccttact
     3361 ttgaagtcat caaggcgttg atctcgcgat cacgtgtgtt ccagctgcga aacctaaatc
     3421 agatagagac aggaattctc attgatcgtg cgctcacgga taaggaacgc gggtatggag
     3481 acaaacgagt ccttctgcac gatgacgccc gctcccattt agttgatgtt gcatctggcg
     3541 acgcccgcaa tgcgttgaac gcgatcgagc tggctgttga atccacacca ccagataagg
     3601 atggtgtcat tcaaattacg ctggatgtcg cgcaggaatc cattcagcgg cgcgcagtgc
     3661 tgtacgacaa agatggtgat gcacattacg ataccatttc cgcttttatc aaatcggtgc
     3721 gtggcagtga tcccgacgcg gctttgtatt ggctcgccaa gatgatctat gccggggaag
     3781 atccacgttt tattattaga cgcctgatta tcctggcggg agaagatata gggcttgcag
     3841 accctatggg cctggtcgtg gtgtcttccg ccgcccaggc ttttgatttc attggcttac
     3901 ccgaaggaat atatccgatc gtcgaagcga cactttacct ggcgacggca ccaaaatcaa
     3961 acagcgcggg tgcatatttc aaagcaatga aaaagatcga agacgaggga caggtgtcgg
     4021 tgccacgcca tcttatggac ggcaatcgcg acgcggccgc gatggggcat ggcaagaatt
     4081 acgtttatcc acatgaccac gaaggtcact ttactccaca acagtattta ccaaagagat
     4141 tattgggtac gtatttttat gcaccctcac aggagggata tgaagcacaa gtgaccgccc
     4201 ggcttgaaat gtggcgcgac gcgcagagaa aagcgttagg aattacgaag gtggaaaaca
     4261 ttcccgctat gacggaagag caaattcaag acatgaagag aaagatcaaa taatcgattt
     4321 ttgcttgatc aaacattaaa ctaataacgc cttctctatg aacctttccc acatagaagc
     4381 aactgtatcg gaatatgatg ttgcgatcat cggcgctgga ccggcaggct cggctgcagg
     4441 ctactttttg gccaccggcg gattaagagt agtcttattg gataaatttg attttccgcg
     4501 cgacaaaatc tgcggcgacg ggctgacgcc acgtgcgata aaaatcctgg caaagatagg
     4561 cattcttcca caactcgaaa aaaccgcatt tcattgctct gaactcaaga ttcgttattc
     4621 tgacgagctg acctttaacc tggggcttga gaatctcgaa aacctgccga attacatttt
     4681 aacaatgccg cgcctacagt tggatcatgc gctccttcaa cacgcgatca acgcaggagc
     4741 aaaatttttt ccaaaggcaa aagtcgagtc gatttccaga gatttcgata agcatattca
     4801 tctggagatt gaatctggac gcaggattga atgtgcgctg gcaattgttg cgacaggagc
     4861 aaataccagg ttattacaaa acttagggtt atcaaagaaa actt
//
LOCUS       DASTJP010000209         7528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_959863, whole genome shotgun sequence.
ACCESSION   DASTJP010000209 DASTJP010000000
VERSION     DASTJP010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959863"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFQ23_06365"
     CDS             <1..363
                     /locus_tag="VFQ23_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906242.1"
                     /translation="PVAPPPTETPLGGGTATPFVLDVPTQTAGAGNPSRTTTDLAVDI
                     RKLSQFRFKVPRDSSLYLRDTSGDGKANSCKITKTGVSISVTMPANGAPSAPFRLPAG
                     EYKLKCSGSNKTARITSS"
     gene            complement(350..3493)
                     /locus_tag="VFQ23_06370"
     CDS             complement(350..3493)
                     /locus_tag="VFQ23_06370"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9906243.1"
                     /translation="MAKVFVSYSRKDIEFAKRLTAELQKSDLDFWIDWEGIPPTVDWW
                     KEIEKGIEEADIFLFLISPDSAKSKICGQEIDTAVKNGKRIIPIVVREIEWQDTPPQV
                     GHLNYIFFSRGDDFDTALGKLLTAIQTDYEWAASHRRLQVKALEWERNGKESGFLLRS
                     KDLVDAETNLARNSSKEPHPTDLQREYALQSRKATDRQRRLLTSISSLGIITMAALAI
                     FGFIKAEQATRQSRISRAGELALQAQLLLSRNTEGGNSTSLLLALEAMRSVSGLSHPD
                     TITAEQALHDALYDMHGTPLIGHTGPVTSVVFSPDGHWLASASHMDNFVLVREIGNLD
                     REPILLNGHDQPINALAFDPNSTWLASGSEDGTVRVWQISDYSSKPKILSIHGGGINT
                     LAFSPDGHWLATGGDDGIAYLWDMKNLSGLVQMFRGHSERINHLAFSPDGHWLATGSD
                     DYTVRMWNLKDTSAEPGILRGHTQLITTLSFSPLGTWLATGSYDSLTYLWNLQDLEAE
                     PYIYSVLDENDFHQAILSLSFSPDEEWLAIGSGDNSIRLVNTQDPQAAPLLLDGHDLG
                     EVNIVAFSSDGRWLASGSNDHDVFIWDVTGADRPITPTAGFRGHDQAVLTLAFSPDGK
                     WIASGSQDHMVRLFEVGNPSIIDPIQLQAYHEPVNIAIFSPHGNWLATGSDDEMVRLW
                     NSKDAATPPRLSGELSGPGPISSVAFSPNEHWLASASYNAPNLFVWNIEESTHTPITL
                     NVEDVTYTVAFSPDGLWLASGGEDHLIRLWNVEDPSALPVLLEGHQEAVSSLAFNRDS
                     HWLASSSTSESTVYLWNMQEPTQSPILLEGHTDVINSISFSPDGHLLATASYDGTLRI
                     WNIESKSSEPLILRGHEDSVNTLAFSPDGLWLASGSDDKTVRLWSMSELAQPPFVLRG
                     SSDSILSLAFSADSKYLAAGENDLEPVIRLWDMNNPEINPVVLRGHDDAVVSLTYSPV
                     EAKLVSASVDTTVRLWNMDIHQLFTMACQTAGRNLTRTEWTQYGFREDYQATCPEWPL
                     REDKESSIEKTSSIMSW"
     gene            complement(3735..6167)
                     /locus_tag="VFQ23_06375"
     CDS             complement(3735..6167)
                     /locus_tag="VFQ23_06375"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HET9906244.1"
                     /translation="MKLNHGKEINPLRKAGTILKNFRLIALWLLVLIFIAGSTLAVVS
                     PAMAHEEEGDCFNEETGEATAIECAEADNAWQEFLAGVELLTDFNQPGEPVINGVSTY
                     TYTPNMTPLGYNRRSVPASGPGNGRFNSDIGAIWGNYAYEGHYDGFRILDISDPTNPT
                     QLINYTDCVGSQGDLVVWENLLIRSWDAPAGATARCGGVLVGNGFEGLSIFDVSNPIA
                     PVLLRNLRMASSGNPAGSPTGCGSHTVLLVPDVGRGNLYVYSMASSGTCGGVDIVRIP
                     LSDPANAVFVRRELSSPGRACHDGGVILGDVNLLSCAGGNGLTTWSFDPSLPANAVGG
                     LEDPIQLWSQSISGVSIGHSATFTWDGKYIVFGHEPGGGSQAQCQSGSSTVNKSIFFF
                     NARTGGAPVGSFVLPRPQTAQENCTWHYYNTVPTNRGYVLVAGNYQSGISVVDFTNPA
                     AATEIAYADPARLSTSGIILGGDWATYWYNGHIYESDITRGLIIWSLDHRLVQGARTL
                     TRYPSTGTQEFSIPLDKTAPVTSISLSPAPVNNWYVNPTVTLTADDGADGSGVDKIEY
                     NLDGGGFVTYSGPFQVTGDGSHTIEYRSSDFALNVEATQSFSFQVDATRPVTSVTGVA
                     QNAVYTLGSVPAAGCSSTDNLSGLAQEATVSVAGGSVNGVGTFTATCSGAADVAGNSA
                     LDAVVTYAVHYAGLSGFLQPINPDGSSLFNRGQSVPAKLQLGGDEFFGYDTSGWTIQK
                     ISAMCGGSTGSVSEDVGSVTPSTQFRYDAASDQYIYNTDFRNAAVGSCWILRLTLDSG
                     QVIDSAMFRIRR"
     gene            complement(6457..7164)
                     /locus_tag="VFQ23_06380"
     CDS             complement(6457..7164)
                     /locus_tag="VFQ23_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906245.1"
                     /translation="MPIKKIPIQRILLINNEPATGVLWVRSLQQQKIEVILEQDPSQA
                     FDRWAKEIPDLIVIDVDIPIGQILYLVKSLRRESIMPILVLLNPMNEGEILDFYHEGA
                     DECIVKPVSRPLFIAKIKAWLRRSWNIPVETLEPFRVGKVQLHPSERTVQINGYKPVR
                     LTNLELRIVLSLMTRAGRTFTAQELIQQIWGYSAEGDRATLKNLVYRLRRKIEDNPAQ
                     PRIIQTVGNAGYKLAAD"
BASE COUNT         1806 a         1943 c         1940 g         1839 t
ORIGIN      
        1 cctgtagcac ctccgcccac agaaacccct ctgggaggcg gaacagctac accctttgtc
       61 cttgatgtcc ctactcaaac agcaggtgcg ggtaatccat cgagaacgac gacagacctt
      121 gcggtggata tcaggaagct gtcacagttt cgattcaagg tacctcgtga cagttcgctt
      181 tacttacgcg atacaagcgg ggacgggaag gccaattcgt gtaaaatcac gaagactggt
      241 gtctcgatct ctgtcacgat gccagcaaat ggagcgccat cggcaccgtt ccgacttcca
      301 gcaggggaat ataagttgaa gtgttcgggc tcaaacaaaa ctgcacggat caccagctca
      361 taatcgagct ggtcttctca atcgaacttt ctttatcttc cctcaacggc cattcggggc
      421 aagtcgcctg ataatcctcc ctgaaaccat attgcgtcca ttcggtgcgc gttaggtttc
      481 ggccggcggt ctggcaggcc attgtgaaca attgatggat gtccatattc cacaagcgga
      541 cagttgtatc aacgctggca gataccagct tcgcctcaac agggctatag gttaaggaaa
      601 caacggcgtc atcgtgtccc ctgagtacaa cagggttgat ctccggatta ttcatatccc
      661 agaggcggat gacgggctcc aagtcattct ctcccgcggc gaggtatttg ctatccgcgc
      721 tgaatgccag cgataagatc gagtcagacg agccacgtaa aacgaacgga ggctgggcaa
      781 gttcactcat tgaccagagc cgaacggttt tgtcgtcgct gccagaggca agccataggc
      841 cgtccgggct aaatgcaaga gtgtttacgc tatcctcatg cccacgcaga atcaggggtt
      901 ctgaagattt tgactcgatg ttccatatcc gcagagttcc atcgtaactg gctgtcgcta
      961 gtaggtggcc atctgggctg aatgagatcg agttgataac atctgtgtgt ccttcaagca
     1021 aaatgggcga ctgtgtgggc tcctgcatat tccaaagata gacagtactt tccgatgtgc
     1081 tgctgctggc taaccagtga ctatcgcggt taaacgcgag acttgaaact gcttcctggt
     1141 gtccctcaag cagcacaggt agagctgaag ggtcttcgac attccaaagg cggatgaggt
     1201 gatcctcccc gccggacgca agccacagac catctggact gaatgcgacc gtataggtca
     1261 catcttcgac gttcaacgta atcggcgtgt gggttgactc ctcgatgttc caaacaaaga
     1321 ggtttggagc attgtaactg gctgatgcca gccagtgctc atttgggctg aatgccacgc
     1381 tcgaaatagg acccgggccc gaaagctcgc ctgacaatcg aggcggcgta gcagcgtctt
     1441 ttgaattcca tagacgaacc atttcatcgt cgctgccagt ggccagccag tttccgtgtg
     1501 gactgaatat agctatgttg acaggctcat ggtaggcttg caattgaatg ggatcgatga
     1561 tagacgggtt gcccacctcg aatagcctga ccatatgatc ctggctgccg gaagcgatcc
     1621 acttcccgtc tgggctgaag gcaagagtca gcactgcctg gtcatgaccg cgaaagccag
     1681 cagtgggtgt aataggacgg tcagcacctg tgacatccca aataaagaca tcatgatcgt
     1741 tgctgccact tgccaaccaa cgaccatccg agctgaaggc tacgatgttc acttccccta
     1801 aatcgtggcc atcaagtaac aagggcgcag cctgtggatc ctgagtgtta acaagacgaa
     1861 tggagttgtc tccactccca atagcaagcc actcctcatc tgggctgaaa gaaagactaa
     1921 ggatcgcctg atgaaaatca ttttcatcga gtactgaata tatataaggc tcagcctcca
     1981 aatcctgcaa gttccataaa taagtgaggc tatcgtaact gccagtggca agccaggttc
     2041 caagtgggct gaatgatagc gtggtgatca actgagtatg ccctctcaga attccaggtt
     2101 cggcagaagt gtctttaaga ttccacatgc gaacggtgta gtcatcgctg ccagtggcga
     2161 gccagtgacc gtcagggcta aaggcaagat gattgatcct ctcgctgtgg cctcgaaaca
     2221 tttgaaccaa gcctgaaagg tttttcatgt cccaaagata ggcgatgcca tcatcacctc
     2281 ccgtggccag ccagtgtccg tcagggctga atgccagagt gttgatgcct ccaccgtgaa
     2341 tgctgagtat ctttggcttg gacgaataat cactgatctg ccacacgcgg accgttccat
     2401 cttcactgcc actcgccagc caggtgctat tcggatcaaa tgccagcgca ttgatcggct
     2461 ggtcatgccc atttagcagg atcggctcgc ggtcgagatt tccaatttcg cgaacgagca
     2521 cgaaattatc catatggctc gcggaggcca accagtggcc atccggactg aagaccacac
     2581 tggtaactgg ccccgtatgc ccgatgagcg gcgtcccatg catatcgtac agtgcatcgt
     2641 gcaaggcttg ttcggctgta attgtgtctg gatgtgacag ccctgacaca gagcgcatgg
     2701 cctccaaagc cagcaggaga cttgtgctat tgccgccctc tgtattccgg ctaagcaata
     2761 actgagcctg aagcgccaat tccccggcgc gtgaaatccg tgattgacgg gtagcctgtt
     2821 cagctttgat aaatccaaag atggccaggg ctgccatggt gatgatgccc aggcttgaga
     2881 tgcttgttag aagtcgtctt tgtctgtctg tggcttttcg gctttgtaag gcatattcac
     2941 gttgaagatc ggtggggtgc ggctccttgg atgaatttct ggcgagattg gtctctgcat
     3001 cgaccagatc cttgcttcgt aataagaaac cgctttcttt cccatttctt tcccactcca
     3061 atgccttgac ctgcaaccgg cgatgcgaag ctgcccattc ataatctgtt tggatggctg
     3121 tcaacaattt tccaagtgct gtgtcgaaat cgtcgccccg gctgaagaag atatagttta
     3181 gatgccccac ttgcggaggt gtatcctgcc actcgatctc gcgcaccaca attggaatga
     3241 tgcgtttgcc gttcttgacg gcggtatcaa tttcctgccc acaaatttta gatttggccg
     3301 aatcgggact gatgaggaac aggaagatat cggcttcttc gattcctttt tcgatttcct
     3361 tccaccaatc cacggtgggt gggatgccct cccagtcaat ccagaaatcg agatcgcttt
     3421 tttggagttc ggcggtaaga cgctttgcga actcaatatc cttgcgtgaa taagaaacga
     3481 aaacctttgc cataaattga tcatactctt gtatctgaga gtctactttg attatcgcag
     3541 ttaatttgct tcacattatc ataaccccaa ttcgcccaat acgtcgtagg gaattgtttt
     3601 caacaacccg agtgaaaata aattcataga aaacaaaaag agtttgagcg atcccgccgc
     3661 ccaaactctt ttttctagaa ctgtttatgt cgctggctgc gctaatacta aacgcagcca
     3721 tcaatcattg cgtgctatct cctgatccgg aacatggctg agtcaatgac ctggccgctg
     3781 tcgagagtaa gtcgcaggat ccagcaggaa cctactgcag cattgcggaa gtccgtatta
     3841 tagatgtatt gatcgcttgc tgcgtcgtaa cggaattgag tggatggtgt aaccgagcct
     3901 acatcctctg ataccgagcc agtagagccg ccgcacatgg cgctgatctt ctggatggtc
     3961 caaccgctgg tgtcatatcc aaagaactca tcaccaccga gttgcagttt ggcaggcaca
     4021 gactggcccc ggttgaacaa gctggaaccg tctggattga tcggctgtag gaatccgctc
     4081 aaccctgcat agtgcactgc ataagtcacc acagcatcga gagcagaatt tccggcaacg
     4141 tcggctgcgc cgctacatgt cgcggtaaag gtgcccacgc cgttgaccga gccacctgca
     4201 acggagaccg ttgcttcctg ggccagcccg gacaggttgt cagtggatga acacccggcc
     4261 gcaggaacag aaccgagtgt atacaccgcg ttttgggcaa cgccggttac agaggttacc
     4321 gggcgtgtgg cgtcgacctg gaagctgaac gattgagtcg cctccacgtt gagggcgaaa
     4381 tcgctggaac gatattcaat cgtgtggctg ccatccccag tcacttggaa tgggccgcta
     4441 tatgtaacga aacctccgcc atccagattg tattcgatct tgtccacccc ggacccatcc
     4501 gctccatcat cagcagtgag cgttacggtg gggttgacat accagttgtt caccggggct
     4561 ggcgaaaggg agatcgaggt cactggcgcg gtcttgtcga ggggaattga aaattcctgc
     4621 gtcccggtgg aggggtatct cgtgagagtc ctcgcaccct ggacgagtcg gtgatcgagg
     4681 ctccatatga tgagcccacg ggtgatatcc gattcgtaaa tgtggccgtt gtaccagtag
     4741 gtggcccaat cgccgcccag gataattcct gatgtactga gtcgtgccgg gtccgcgtag
     4801 gcgatttccg ttgcagcagc cgggttcgtg aaatcgacga cggaaatgcc agattggtaa
     4861 ttacctgcga caagaacata accgcgattg gtcggtactg tgttgtagta gtgccaggtg
     4921 cagttctcct gggcagtttg cggccttggc aggacgaagg agcccactgg tgccccgcca
     4981 gtgcgggcat tgaagaagaa gatggacttg ttgacggtgc tgctgcctga ctgacactgc
     5041 gcctgactgc cgcctccggg ctcgtgaccg aagacaatat actttccatc ccaggtgaat
     5101 gtggcagagt gcccgatgga aactcctgaa atagattgtg accagagctg tatcggatct
     5161 tcaaggccgc ctacagcgtt tgcaggaaga ctgggatcga aactccaggt agtcagaccg
     5221 ttaccgccag cgcacgaaag cagattgacg tctcccagaa tgacaccacc gtcatggcag
     5281 gcacgaccag gactggaaag ttccctccgc acgaagactg catttgccgg gtctgacagc
     5341 ggaattcgga cgatatccac gccgccgcat gtgccactgg atgccatgct atagacatac
     5401 aaattgccgc gtccaacatc aggaacgagc agaacagtgt gagatccgca gccagtcggg
     5461 cttccagcag ggttaccgga agaagccata cgcaagttgc ggaggagaac tggtgcaatc
     5521 ggattgctga catcaaaaat gctgagtccc tcgaaaccgt ttccgactag cactcccccg
     5581 caccttgcag tggcgcccgc tggcgcatcc caggatcgaa tcagcagatt ttcccagacg
     5641 accaggtcac cttgcgaacc gacacagtct gtataattga tcagctgggt ggggtttgtg
     5701 gggtccgata tgtctaagat tcggaagccg tcataatgcc cttcataggc ataattgccc
     5761 cagatcgcgc cgatgtctga attgaagcgc ccgttgcccg gacctgatgc cgggacactc
     5821 cgtcggttat agcccaacgg ggtcatattt ggcgtatatg tataagtgct tactccgttg
     5881 atcacaggct caccaggttg gttgaagtcc gttaagagtt ctacacctgc caggaactcc
     5941 tgccaggcat tgtcggcttc tgcgcactca attgcggtag cttcgccggt ctcttcatta
     6001 aaacaatccc cttcttcctc gtgtgccatg gcaggtgaaa caaccgccag ggttgagccc
     6061 gcgatgaata tgagtactaa aagccatagt gcaattaacc tgaagttttt caaaatcgtg
     6121 ccagctttcc ttaagggatt gatttctttc ccgtggttca gtttcatttt attacattga
     6181 tcctttcgag gaatttttcg ctttactcaa agtttcaaat gtttaagctt cggaaccgcc
     6241 ctgcaaatca atttaatctg gtgctgctcc tttccaaggc tcgaaacaca ggaataaagg
     6301 ctgcaaaatg caacttgcat ccaaaacaaa accgccttat aagttttgtt taaggatact
     6361 tcatattatt ttaatatgat ggggtcacaa atcggtcaca atttatgcgc gattcgtcaa
     6421 attaattgac ccccactact gatttgatta cttaacttaa tctgctgcga gtttgtagcc
     6481 ggcgttcccg accgtctgta taattcgagg ttgcgccggg ttgtcttcaa tcttacggcg
     6541 tagacggtac accagatttt taagggttgc gcggtcacct tccgcgctgt agccccagat
     6601 ctgctgaatc aattcctgcg cagtgaatgt acggccagcc ctggtcatta aagagagcac
     6661 gatgcggagc tccaggttgg tcaacctcac cggcttatat ccgttgattt gtaccgtccg
     6721 ttctgatggg tgcagctgca ctttgcccac tcggaacggt tcaagagttt caacaggaat
     6781 gttccatgag cgacgaagcc acgcttttat cttggcgatg aacaagggac gactgactgg
     6841 tttgacaatg cattcgtctg ctccttcgtg gtaaaaatcc aggatttcac cctcgttcat
     6901 agggttgagt agcacgagaa tgggcataat tgactccctg cgcaggctct taaccaaata
     6961 aagaatttgc ccgataggaa tgtctacatc aatgacaatt agatcaggga tctcctttgc
     7021 ccagcgatca aaagcctgag atggatcctg ttccaaaata acctcgattt tttgttgttg
     7081 aagagagcgg acccacagca cccctgtagc tggttcattg ttgatgagaa gtattctctg
     7141 gatggggatt tttttaattg gcattttcta ctctttcgca acaggacccg ctcgttgctg
     7201 gcgttaaatt tttgatgctt gcagtgggaa ctgattgaga gcgattatac tggcaaagaa
     7261 taaaggcctg aacacttgtt tatcaacgga gtggttaaga gaatgagtga aaatctactt
     7321 agccacagag agcactgagt cctttgaccc ctcacctgcg ttcggggcag gtggaaaaaa
     7381 ctcaaaaact ctgtgggctc ccctagcgcc gcccgtgatt tgctcaagca aatcatgtag
     7441 gtgtgtggct tacacaccaa attcgccgct ccctttatca agcccggtac gaatcaaggc
     7501 gagaatctga cgaactttgg cggcaggt
//
LOCUS       DASTJP010000210         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_990306, whole genome shotgun sequence.
ACCESSION   DASTJP010000210 DASTJP010000000
VERSION     DASTJP010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990306"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFQ23_06385"
     CDS             complement(<1..265)
                     /locus_tag="VFQ23_06385"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9906246.1"
                     /translation="MRRDVFQAIADPNRRAIIGLLAKQKLTVNAVAEHFSISRPAVSR
                     HIKVLKECGLVMIRRQGRERYCELHLEKLNEVAAWVEKYQKVWE"
     gene            complement(532..1248)
                     /locus_tag="VFQ23_06390"
     CDS             complement(532..1248)
                     /locus_tag="VFQ23_06390"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET9906247.1"
                     /translation="MTAKLNADEFLKKLEALRSSKIAKAHDHLASDEDDVILGVRMGQ
                     VFALAKEFMGMPLDEVGKLLESPIHEARVGAVSIMDFQARSKKTTKARRKELFDLYIK
                     RHDRINTWDLVDRSAPYVVGGYLFDKPRKILYKLARSKKMPERRTAIVSTGYFIRQGD
                     VDDTFKIAEMLIDDEHVLIHKATGWMLRAAGGVDRQKLVNFLDTYAGSMPRTTLRYAT
                     EHFDKKQRDRYLNLKKDRGL"
     gene            complement(1292..1912)
                     /locus_tag="VFQ23_06395"
     CDS             complement(1292..1912)
                     /locus_tag="VFQ23_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9906248.1"
                     /translation="MSKVVLELSMSLDGFIAGPNDNPDNGLGDGGDSLFKWYSSGDTD
                     FPLPGTDMVFKISRASAEFLRPSWLNIGASVTGRRTFDIARGWGGNPPGGADAPHFVV
                     THTVPQEWVKPSSPFTFVTDGVESAVRQAKKAAGDKNVDLIGASIAQQCLKAGLLDEI
                     QIDLAPVLLGGGVRLFDHLDTVPIELEPLAVVQGLDVTHLRYRVVK"
     gene            complement(1932..>2733)
                     /locus_tag="VFQ23_06400"
     CDS             complement(1932..>2733)
                     /locus_tag="VFQ23_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9906249.1"
                     /translation="TSMKTVTSKDGTTIAFDQCGAGPTVILVDGALQYRAFDQGMTEL
                     ADLLAKDFTVIHYDRRGRGDSTDTQPYALDREIEDIEAIIDEAGGSASLYGVSSGAAL
                     AMEAAIKLGDKVSKLVMYEAPYNNDEAAQQAWRKYVKQLKELLDQGRKGDAVGLFMML
                     VGASPDDVEGIKQSPMWPLWEAVGPTLAYDHIAALGEDAAIPTARAARVSVPALVMDG
                     SESFPFMHTTAVALAKAMPKGQHRTLEGQTHEVSAEALAPVLIEFFKA"
BASE COUNT          648 a          759 c          630 g          696 t
ORIGIN      
        1 gttcccagac tttctgatat ttctcgaccc aggctgccac ttcgtttaat ttttccaggt
       61 ggagttcaca gtatcgctcg cggccctggc gccggatcat cacgagtcca cactccttca
      121 aaactttaat gtgcctggaa acggccggac ggctgatgga aaagtgctct gcaaccgcgt
      181 ttacagtcag cttttgcttc gctaaaagac cgatgatggc tctgcgattg ggatcggcaa
      241 ttgcctggaa aacgtctctt ctcatgctct atatgtaacc aaactattac atattttacc
      301 aattttagaa tatttgttct attatgtcaa ggcgaaaatg ggttaattta tgaaattgtt
      361 gaatgcgcgc tgataagaac gaagagataa tcaggggtgt tacggtgcaa ttaacaaacc
      421 cttgcatcca acttcatggt acgcgtgccg aaagcacaat ttacttgtca caagtatcaa
      481 tcaaacattc atagtccccg gcggtaagcc ttgagcattt ccaatctttt gttacagacc
      541 ccgatccttt tttagattca aataacgatc cctctgtttt ttatcaaaat gctccgtggc
      601 atagcgcagg gttgtacgcg gcatgctgcc agcatatgta tccagaaagt tcactaattt
      661 ttgccggtcc accccgcctg ccgcccgcaa catccagcct gtcgccttgt ggatcagaac
      721 atgttcgtca tcaatcaaca tctcagctat cttgaacgta tcatccacat cgccctgccg
      781 gatgaaatag ccggtgctga caattgcagt gcggcgttcg ggcatctttt tggagcgcgc
      841 cagtttatac aggattttgc gcggcttatc gaacaggtaa ccgccaacca catagggcgc
      901 gctgcgatct accagatccc aggtattgat ccgatcatgg cgtttgatat acagatcaaa
      961 cagttctttc cggcgcgctt tcgtggtttt cttggaacgc gcctgaaaat ccatgatgct
     1021 gaccgcgccc actctggctt catggatcgg actctcgagc agtttcccaa cttcgtcaag
     1081 cggcataccc ataaattcct tggcgagcgc aaagacctgt cccatgcgta cgcctaaaat
     1141 gacatcatcc tcatccgagg caagatgatc gtgggctttg gcaattttgg atgaacgaag
     1201 cgcctctaat tttttgagaa attcatccgc attcagcttg gcagtcatgt ccgatacctt
     1261 tcacaaaagg gtattgagag aagacccatc gttattttac gacacgatat cgaagatgtg
     1321 tcacatccag cccctgtact acggccagag gttcaagttc aatgggtaca gtatccaaat
     1381 ggtcgaataa tcgcacgcct cccccgagca ggacgggggc caggtcgatc tgtatttcat
     1441 ccagcaaccc tgccttgaga cactgctgtg caatacttgc gccaatcaaa tcgacgttct
     1501 tatctcccgc ggcttttttc gcctgacgga cagcgctctc aacaccatcc gtgacaaagg
     1561 taaatggtga actcggcttg acccattcct gggggactgt gtgggtcacc acaaaatgag
     1621 gagcgtccgc gccacccgga ggattgccgc cccaaccccg agcgatatcg aaggtcctcc
     1681 gccccgtgac cgatgcccca atatttagcc aagatggtcg aaggaactca gcactggcgc
     1741 gcgagatctt gaataccatg tctgtgccgg gcagcggaaa atcggtatcg ccgctggagt
     1801 accacttaaa gagagagtca ccgccatcgc ctaaaccgtt gtcaggatta tcgttcggtc
     1861 ccgcgataaa accgtccagc gacatcgata actctaagac tactttactc atatccatgt
     1921 ttccttctac atcaggcttt gaaaaactca attaacacag gagcaagcgc ctccgcagac
     1981 acttcatgtg tctggccctc cagggtgcgg tgctgccctt tgggcatcgc tttagcaagc
     2041 gccaccgctg tggtatgcat gaacggaaaa ctctcacttc catccatgac aagcgcgggc
     2101 acggaaacac gggcagcacg cgcggtggga atagctgcat cctcaccaag agcggcaatg
     2161 tggtcgtagg ctagcgtggg tcctaccgct tcccacagcg gccacatggg actctgtttt
     2221 attccttcca catcatcagg agatgctcct accaacatca taaagagtcc caccgcgtcg
     2281 cctttgcgac cctgatccag tagttccttt agctgtttta cgtatttcct ccatgcctgc
     2341 tgagcagcct catcattgtt atatggtgct tcgtacatga ccagtttgct gactttgtct
     2401 ccaagcttga tcgctgcctc cattgccaga gctgcgccgg aggagacgcc gtacagagat
     2461 gccgaaccac ccgcttcgtc aatgatggct tcgatatctt ctatttcacg gtcaagcgca
     2521 tagggctgag tatcggtgct gtcgccgcgt ccacgacgat cgtagtgaat aaccgtaaag
     2581 tctttcgcca atagatctgc cagttccgtc attccctggt caaacgcccg atattgcagc
     2641 gcgccatcta ccaggatgac cgtcggtcct gcaccgcatt gatcaaacgc aatggtcgtg
     2701 ccatcttttg aggtaactgt tttcatagaa gtt
//
LOCUS       DASTJP010000211         2826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1289512, whole genome shotgun sequence.
ACCESSION   DASTJP010000211 DASTJP010000000
VERSION     DASTJP010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289512"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..2066
                     /locus_tag="VFQ23_06405"
     CDS             48..2066
                     /locus_tag="VFQ23_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9906250.1"
                     /translation="MNFKFTPVARLALALIILMTSIAAAPFSQTHRPQRSATQDLLDE
                     LGGFPCFEDSWFTCVTIEMPLDHFNPSDTRTIPVTFAVLPASGDRKGMFVTATGGPGY
                     SGIASADSYTAAFDPGIAEVFDIVFFDQRGLLLSGDQTCPFAASAYYQQESSGLTSEE
                     EAALKQTAETFSTDCVNEVSNPELLPYLGTKQAVEDLEVFRQLIGDDKFWLYGESYGT
                     QYAQTYAAAHSEHLAGLILDGTVDLTYDGIAYYTLQAQAFSDILTASLAACNDDPACN
                     ADLLGDAIVAYDNLAAQLSQGSISFRFPLPLGGFERRTFTLSNLEFVAASQMYTESDR
                     MIFVRALAAYASNGSLVSLARLLYLDLGLDPQTLDIILDPYYSDAVFFGVECQDYGYP
                     GSTPDEKAENYLNAADAVEISIPRLASIIYGDFPCAYWPYATSDLARPDYLTLPDVPT
                     FVLGATADPATPVGNGINVFQHLANGYLITQQGGPHVIFGRGNECPDLIITDFLVNDV
                     VPAEREITCDGFLMDEYVPISPRFAAAFKTPRNALSAVETEIYYLPEYFYWDGFTSTD
                     VGCTYGGTLNFTTNNAGTRYLFNLDKCAFTTNFIMTGSGFYNLNNDRFVLEVKTTGRW
                     KCDLKYVRSGERINITGRCDGKSINENRNDDDRDKHIAPDHRENKEDR"
     gene            2135..2398
                     /locus_tag="VFQ23_06410"
     CDS             2135..2398
                     /locus_tag="VFQ23_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906251.1"
                     /translation="MSIPDRIITMAELRRNTGERGTRKFIAYDGIIYDVTDCPKWRLD
                     LHENLHFPGQDLTSELPEAPHKEEVFSRPCVKAVGKLQSYDGY"
     gene            2493..>2826
                     /locus_tag="VFQ23_06415"
     CDS             2493..>2826
                     /locus_tag="VFQ23_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9906252.1"
                     /translation="MNQKFILTVGYYLAFILLGLSIGAEGPTLPKLAENTSSALDQIS
                     SIFIFGSLGYLFGSYISGRFYDRVPGHRVMSGVLLLLGISVALVPFATSLNLLLLIIL
                     IAGLAKGAI"
BASE COUNT          672 a          697 c          678 g          779 t
ORIGIN      
        1 ataaatcgac gtatttcaag tgcaatcaac ctcaaaggaa gcggcgaatg aatttcaaat
       61 ttactccagt agcacggctg gctctagctc ttattatatt gatgacgtcg attgctgccg
      121 cgccgttttc tcagactcat cgtccccaac gcagcgcaac acaagacctt ctcgatgaac
      181 tgggcggctt tccttgtttt gaggatagtt ggttcacctg tgttacgatc gaaatgccgc
      241 tcgatcattt caacccatct gatacgcgta ccattccagt gacatttgct gttctaccgg
      301 cctccggtga ccggaagggg atgttcgtca ctgcgacagg cggaccgggc tactctggca
      361 ttgcctcagc tgactcctat acagctgcat ttgaccctgg cattgccgaa gtttttgata
      421 tcgttttctt cgatcagcgc gggcttctgc tttcgggcga tcaaacctgt ccgtttgcag
      481 ccagcgcgta ttaccagcag gaatccagtg gattaacttc ggaggaggaa gctgccctta
      541 agcagactgc cgagacgttc agcacggatt gtgtcaatga ggtcagcaac ccggaacttt
      601 tgccatatct gggtacgaag caggccgtgg aagatcttga agttttccgt caactgatcg
      661 gggatgacaa attctggctg tacggcgaaa gctatggaac gcaatatgca caaacctacg
      721 cggcagcgca tagtgaacat cttgccgggc tcatcctgga tggaaccgtc gacctgacct
      781 atgatggaat tgcttattac acgttgcaag ctcaggcatt tagtgacatc ttgactgctt
      841 cactcgctgc ctgcaacgat gacccggctt gtaatgccga tttgcttggc gatgcgattg
      901 tggcgtatga caatttagca gcacaattaa gtcagggatc tatttctttt cggttcccac
      961 ttcctttagg tggctttgaa cgaaggacat ttactctctc gaatcttgaa ttcgtggctg
     1021 ccagccagat gtacactgag agtgaccgga tgatctttgt ccgtgcgctg gccgcctatg
     1081 catcaaatgg ctcgctggtt tcgctggcgc gtttgcttta cctggatctt ggtttagatc
     1141 cacaaacact ggatattatc cttgatccct attactcgga tgctgtgttc ttcggtgtcg
     1201 agtgtcagga ttatggatac ccgggtagca ctcccgatga aaaagcagag aattacctga
     1261 atgcggcaga cgctgtagaa atttccatcc cacgacttgc ttccatcatc tatggcgatt
     1321 tcccttgtgc ctattggccg tacgccacct ctgatctggc ccgcccagat tatctgaccc
     1381 tgcccgacgt tccaacgttt gtgctcggcg caaccgctga cccggctact ccggtgggca
     1441 atggcatcaa cgtctttcag catcttgcga acggttacct catcacccag cagggcggac
     1501 cccatgtgat ctttggacgc ggcaatgaat gccccgacct gatcatcacc gattttcttg
     1561 tcaatgacgt agtccccgca gagcgtgaaa tcacctgtga tggcttttta atggatgagt
     1621 atgtgccgat atccccgcgc tttgctgctg cgttcaaaac accgcgcaat gcattatctg
     1681 ccgtagagac agaaatctac tatctccccg aatatttcta ctgggatggc tttacgtcca
     1741 ccgatgtagg ttgtacgtat ggtggaacct tgaactttac gacgaacaat gcaggtacgc
     1801 gttacctttt caatctggat aagtgtgcgt tcactaccaa cttcattatg acgggaagtg
     1861 gcttttataa cctgaataat gatcgctttg tcctggaggt aaaaacgacc ggacgctgga
     1921 agtgcgatct aaaatacgtg cgctcgggcg agaggatcaa tattaccggc aggtgtgacg
     1981 gaaagtccat caacgaaaat cgcaatgatg atgatcggga taagcacata gcccccgatc
     2041 acagggaaaa taaagaagat cgctaaccca gataagaaat ctcctgtatc acacaggaga
     2101 ttttctttta tcaatcccct gtataattcc gtgaatgagc attcctgacc gcatcatcac
     2161 catggcagaa ctacgacgaa acacagggga acgcggcaca cggaaattta tcgcttatga
     2221 cggcatcatc tatgatgtaa cagattgccc gaagtggagg ctggacctgc acgaaaacct
     2281 gcattttcca ggtcaggatc tcaccagtga acttccggag gcgcctcata aggaggaagt
     2341 cttttcgagg ccctgcgtga aagcggttgg taaattgcaa tcgtatgatg gatattgata
     2401 acaatcaaga ttagtccttt tcattggttt aacaaccaat cggactgttt tgctaatgtt
     2461 gggataatat aactctagtc aaaggtcact cgatgaatca aaaatttata ctcacggttg
     2521 gttattatct tgccttcatc ttgctcgggc ttagtattgg agctgaggga cccacacttc
     2581 caaaactcgc agaaaatact tccagcgctc tcgaccagat cagctcgata tttatctttg
     2641 ggtcattggg ttatttattt ggctcgtata ttagtggtcg tttttatgac cgcgttccgg
     2701 ggcatcgggt aatgtctggt gttcttttgt tgttgggaat atctgttgca ctcgtgccct
     2761 ttgccacatc attaaacctg ttacttttga ttatcctgat cgctggcttg gccaaaggtg
     2821 ccattg
//
LOCUS       DASTJP010000212         7649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1216402, whole genome shotgun sequence.
ACCESSION   DASTJP010000212 DASTJP010000000
VERSION     DASTJP010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216402"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFQ23_06420"
     CDS             complement(<1..570)
                     /locus_tag="VFQ23_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906253.1"
                     /translation="MFELKRTPFHERTSKLCLPQNWRRWAGYIVVGSYELTLDREYWA
                     IRNSAALIDVTPLMKYILKGKDASRLLNRIVTRDMDKMKVGQVYYTGWCDDEGKMIDD
                     GTISKLDETTFRMTSADPSLRWLSMNSVGLDVDVTEVTDQVAALSFQGPNSRKILNKV
                     ADKPVDELKYFRLMQNNVDGIPVTISRTGY"
     gene            complement(812..1891)
                     /locus_tag="VFQ23_06425"
     CDS             complement(812..1891)
                     /locus_tag="VFQ23_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906254.1"
                     /translation="MAFQLEKFFVDVFAPQRSDVLTIMYDLPQDDVQDNLAWQERRAL
                     ADEWHQKINTFAQSYGFRVNPVVTYEATGAHARDLPEFGQSEGNTVRLEDVARDSTIL
                     ISMPQFSATAPLLILTKKYENLRVASMPLVSKTMEETGLSADYNKVARTCAKLAPLFE
                     QSDGIEVTFSTGHKCYFDKSDHKPARQDNGRLHPGKEALRLRNFPSGEVWVVPNESPE
                     SKTSGEIPVSYGDETAIFVVERNMIVDVRGDGPMARQRRQEFREEKALSNVAEVAIGC
                     NDKAVVTGNPLEDEKAGLHWAYGRSEFLGGTVAPKDFSAPDKVLHQDIVYAKGNPIVC
                     ERFDFIFPDGTRKTAIVDGRLKIED"
     gene            complement(1902..2228)
                     /locus_tag="VFQ23_06430"
     CDS             complement(1902..2228)
                     /locus_tag="VFQ23_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9906255.1"
                     /translation="MQSFELAPLLSQRESSNKLYLEFLKVPDLSMGIYVLPAGGTDPQ
                     SPHTEDEVYYVVSGKGKVLVADETRDVQAGSIVYVAKNIAHRFHSIEEELTLLVFFAP
                     AEYSNK"
     gene            complement(2425..3321)
                     /locus_tag="VFQ23_06435"
     CDS             complement(2425..3321)
                     /locus_tag="VFQ23_06435"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HET9906256.1"
                     /translation="MMNNKIIAVTGATGQQGGAVARKLLADGWKVRALTRDTDKPAAQ
                     GLKSVGAEVVPGDMENRAELEAAFKDVYGVFSVQNFWIPGVGYEGEIQQGKNVAEVAK
                     AAGVQHLVYSSVGAAHRGMGQKHFESKWIIEQYLQSLDLPYTILRPAFFMENYNWSRA
                     QILNGIFTGMGLRPEKEIQSITVEDIGAFVALAFANRDDYLGKTIELAGDALTEAQIA
                     ETFTKVIGRVVNLTPPSGGGWGDPDEAAAAFNFFNGEAYDADIPALRNLHPGLLTLER
                     YLRRNGWENAEPIPIPANSNWG"
     gene            complement(3308..3661)
                     /locus_tag="VFQ23_06440"
     CDS             complement(3308..3661)
                     /locus_tag="VFQ23_06440"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HET9906257.1"
                     /translation="MSVRGAQETITKAVTAWAGVTVQPHRFGGVEYGIGKREIGHIHG
                     DQLVDIPFPKKVRDEIVATGRAQPHDILPETGWVSFYLYEEDDVEKAIALLHESYQIA
                     QKQKSKTIKEVNDEQ"
     gene            complement(3658..4113)
                     /locus_tag="VFQ23_06445"
     CDS             complement(3658..4113)
                     /locus_tag="VFQ23_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9906258.1"
                     /translation="MPTHFTGTRAAMRTLDTFIKLSRCHDSVMARLAAQNTIGDLTFS
                     QFAVMEALYHLGHLTQGEISSKILKSTSNITTVIDNLERDGLVRRERDANDRRVIHVH
                     LTEAGTRKLESVFPAHVAALVEIFSILTASEQQELGSLCKKLGKGMESE"
     gene            complement(4224..5147)
                     /locus_tag="VFQ23_06450"
     CDS             complement(4224..5147)
                     /locus_tag="VFQ23_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HET9906259.1"
                     /translation="MRKFVLPIAFILPSLACSLGQNSPTATPQPTASQTTTATPSAVP
                     FPLYGEAVADLPYCTAGASLQKLDLYFPDSGGPWPVLAYVHGGSWMHGDKTEALLFVD
                     RMTSEGYLVASINYRLYPEGKFPNMIEDVKCAIRFLRAHASEYNLNPNRIAAIGPSAG
                     GHLVSLLGTSDVNAGWDVGEYLDQSSRVQAVVAMAPVTDLTRSFPNADIEAMRGVGFG
                     EDNIVQASPVTHVTTDDPPFLLIHGDQDTVVPFEQSQLMYDRLVQASVPAQLVAVQRG
                     GHSFTAPDGSATPTFDEINQIIITFLAQHLN"
     gene            complement(5293..6009)
                     /locus_tag="VFQ23_06455"
     CDS             complement(5293..6009)
                     /locus_tag="VFQ23_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HET9906260.1"
                     /translation="MNPTDFIKLKNVLTHGDFEIKGQFMLGSNYTFLVDVNYQDATYP
                     AVYKPSLGEQPLWDFPSHTLAQREVAAYLISECLGFNFVPFTTLRKEGPHGEGSLQQF
                     IEYDPEYHYFNFTEEDRDLLRPVVLFDLLCNNADRKGGHVFFEAGTHKLFAIDHGICF
                     HEDDKLRTVLWDFSGQKIPADLLSRLRFAVDPSLLADLERYLSPGEISALRARADTII
                     KRGTFPRQPRDRRALPWPPL"
     gene            complement(6028..6519)
                     /locus_tag="VFQ23_06460"
     CDS             complement(6028..6519)
                     /locus_tag="VFQ23_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906261.1"
                     /translation="MDTLRDKLSSAIHLRETGKHEEARELLLELHSEFPEDAQINFQC
                     AWVHDLLGLEREAIPFYESAVQAGLSGEDLQGALLGMGSTYRCIGEYQKAKETFQRAL
                     ELFPESNEYKVFLAMTLHNLKDHTNAMGLLLTSLLDTTKDDGILSYERALRFYADKLD
                     ETW"
     gene            complement(6584..7111)
                     /locus_tag="VFQ23_06465"
     CDS             complement(6584..7111)
                     /locus_tag="VFQ23_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HET9906262.1"
                     /translation="MPRFEIDVDPCDHITADAIGNPGQRVFYLQAYQDQRTITVIIEK
                     TQLHSLSIGVEQFLSQINEQNPDVEEASGDYVEDVMRINPPVDPLFRVGEIGLGYDKD
                     RDLIVLFVKEILTEEADPETAAVIRFWASRTQVRMLARWGVEIVARGRPTCPQCQQPM
                     EPEGHFCPKKNGHMH"
     gene            7270..>7649
                     /locus_tag="VFQ23_06470"
     CDS             7270..>7649
                     /locus_tag="VFQ23_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9906263.1"
                     /translation="MYDKKKLDEMKDSLKNWEETSLKKALSSLPERADEFITTSSEPI
                     NRLYTPLDVADQDYASSLGLPGEYPFTRGVHPTLHRSKLWTMRMFAGFGTAEETNARF
                     KYLLDQGQTGLSIAFDLATLMGYDT"
BASE COUNT         1847 a         1980 c         1859 g         1963 t
ORIGIN      
        1 ataccctgtg cgcgaaatcg tgacaggaat accatccacg ttgttttgca tgagacggaa
       61 atacttcaac tcatcaacag gcttatcggc aactttattc agaatctttc tggagttggg
      121 tccctgaaaa ctaagcgccg cgacttgatc cgtaacttcg gtgacgtcaa catcaagccc
      181 aacggaattc attgacaacc atcgtaacga tgggtctgcg gaggtcatgc gaaaggtcgt
      241 ctcgtcgagt ttgctgatcg tgccatcgtc gatcatcttg ccttcatcgt cgcaccagcc
      301 cgtgtaatag acctgaccca ctttcatctt gtccatgtcc cgggtgacga tacggttcaa
      361 caagcgagat gcatccttgc ctttgaggat gtacttcatc aaaggagtca catcgatcaa
      421 cgccgcagaa ttgcggatcg cccaatactc gcggtcgagg gtcagctcat aggaaccgac
      481 gacgatgtat cctgcccagc gccgccagtt ttgaggcagg cagagtttcg atgtccgttc
      541 gtggaaaggg gtgcgtttta gttcaaacat aagattcctt ttgaaccgcc aagcacgcga
      601 agatcgcaaa gaaaaacttc tctaaatctt agcgtccttg gcgctctttg cggttaagtt
      661 cttcacctat ttgaatactt tgctggcgcg aagaagacta atcttagact tccgaagtct
      721 agatactatg ccgccagttt tgagacaggc agagtttcga tgtccgttcg tggaaagggg
      781 tggtcttgag ttggaacata atattctctt ttcagtcttc aatcttcaac ctgccgtcta
      841 caattgctgt cttacgggtt ccatccggga aaatgaaatc gaatcgttcg caaactattg
      901 gattgccttt tgcataaaca atatcctggt gtaacacttt gtctggcgca gaaaaatctt
      961 tgggtgcgac cgtgccgcca aggaattcgc ttcgtccata tgcccaatga agtcctgctt
     1021 tttcgtcttc aagcgggttt ccggtgacga cagctttatc attacagcct attgctactt
     1081 cggcaacatt acttaaggct ttctcttcac gaaactcctg acgtctttgc cttgccatcg
     1141 gcccatcgcc gcgaacatcg acaatcatat ttctctccac gacaaatatg gcggtctcat
     1201 ctccataact cacagggatt tcgccgctag ttttgctctc cgggctttca ttaggaacca
     1261 cccatacttc acccgaagga aagtttctca agcggagggc ctctttgcca ggatgcaagc
     1321 gaccattgtc ttgccgtgct ggtttatgat cagatttgtc aaagtaacac ttatgtccgg
     1381 ttgagaacgt cacctcaata ccgtcggatt gttcaaatag cggcgctaat tttgcacagg
     1441 ttctggcaac tttgttgtag tcagcagaaa gtcccgtttc ttccatcgtc ttggaaacta
     1501 aaggcatgct tgccacgcgc aaattttcgt attttttggt taatatcagg agtggcgcag
     1561 tagcagagaa ctgaggcatg gaaataagaa tggtagagtc cctcgcgaca tcctccaggc
     1621 gaactgtatt cccctcactt tggccgaatt ctggcaagtc gcgggcatgg gcgcctgttg
     1681 cttcgtacgt aacaacagga ttaactctaa aaccataact ttgggcaaat gtgtttatct
     1741 tctgatgcca ttcatcagcc agtgcgcggc gttcctgcca ggcgagatta tcttgtacat
     1801 cgtcctgcgg caagtcatac atgatcgtta atacatcact cctctgaggc gcaaagacat
     1861 caacaaaaaa tttttccagt tgaaaagcca tggtgaatat cctacttatt tgaatactcc
     1921 gctggcgcga agaagaccag taacgtcaac tcctcttcaa tcgaatggaa gcgatgcgcg
     1981 atgttcttcg ccacatacac aatcgagcca gcttgtacat ctcgggtttc gtcggcgacc
     2041 aggaccttcc ctttgccgct caccacataa tagacctcat cttccgtatg cggagactga
     2101 gggtcggtgc ctcctgcggg gagaacataa atccccatgc tgaggtcggg gactttgagg
     2161 aattcaaggt acaacttatt gctggattct ctctgagaaa gcaatggagc gagttcgaag
     2221 gattgcatgg gtctcctttt cagatctttg cgaattcaga agggcgaagc aatctcctca
     2281 tttatgagaa tgccttatca tattttgaga ttgccacgtc gcagcttcgc tgctcctcgc
     2341 aatgacagat caacttgaaa ttatatataa ttttacctgt gttgacttaa aagaaaaagg
     2401 actggccgtg gccagtcctt tcgcttaacc ccagttactg tttgcaggga ttggaatagg
     2461 ctccgcattt tcccagccat tccggcgaag atatctttcc agcgtgagca agcccgggtg
     2521 aaggttgcgc agcgctggaa tatctgcatc ataggcttcg ccattgaaaa agttgaacgc
     2581 agccgccgct tcgtccgggt cgccccatcc gccaccactg ggcggagtga gattcaccac
     2641 tctgccaatg acttttgtga acgtttcagc aatctgcgct tctgtcaacg catcacctgc
     2701 aagttcaatg gttttcccga gatagtcatc gcgatttgca aaggcgagcg cgacaaacgc
     2761 tccgatatcc tccaccgtga tgctctggat ttccttttca gggcgtaagc ccatgccagt
     2821 gaatatccca ttcagaattt gtgcccgact ccagttatag ttctccatga aaaacgcggg
     2881 gcgcaaaatg gtataaggca gatccagtga ctggaggtat tgttcaatga tccatttgct
     2941 ttcgaaatgt ttttgtccca tcccgcgatg tgctgcacca acggatgaat acaccagatg
     3001 ctgaacaccc gcggccttgg cgacctcggc tacgtttttg ccctgctgga tctctccctc
     3061 atatccaacg cctggaatcc agaagttctg cacgctgaag acgccataaa catctttgaa
     3121 cgccgcttca agttcggcgc ggttctccat gtcacccggc accacctccg cgcctacgga
     3181 tttcagtccc tgcgcagcgg gcttgtccgt atcgcgcgtc agtgcgcgga ccttccaccc
     3241 atctgccaat agcttccttg ccacggctcc accctgttga ccggtcgcgc ctgtgactgc
     3301 aataatttta ttgttcatca tttacctctt ttatagtttt cgatttttgt ttttgagcga
     3361 tctgataact ttcatgaagc aaagcaatag ccttttcgac atcatcctct tcatacagat
     3421 aaaaacttac ccagcctgtc tcgggcaaaa tatcatgagg ctgcgcgcgc cctgtagcaa
     3481 cgatctcatc gcgaactttt ttcggaaagg gaatatccac aagctgatcc ccatgaatat
     3541 gcccaatttc gcgtttgcca ataccatatt ccacgccacc gaagcgatga ggctgaaccg
     3601 tcacgccagc ccaggctgtg accgcctttg taatagtctc ctgtgcgccg cgtacgctca
     3661 ttcggactcc atccctttgc ctaatttttt gcacaaggac ccgagttcct gctgctcgct
     3721 ggcagtcaga atactgaata tctcgaccag agcagccaca tgagccggga agacggactc
     3781 taacttgcgt gtgccggcct ccgttaaatg gacatggatc accctccggt cgttcgcatc
     3841 gcgttcgcgc cggaccagcc catctcgttc caggttgtcg atcacggttg taatattgct
     3901 tgtacttttc aatattttgc tgctgatttc accctgagtc agatgcccaa gatgatacaa
     3961 cgcctccatc acggcaaatt gagagaaggt caggtcaccg atggtgtttt gggctgcgag
     4021 acgtgccatc accgaatcgt ggcaacgtga cagtttgatg aaggtatcca gtgtgcgcat
     4081 cgccgcgcgg gttcctgtga agtgagtagg catatattgt ccgatgtcga atagtttgat
     4141 atcgaaatat tatcgcagga aactggcatt gtcaagccag cgagacctgg ccattcgacc
     4201 aggtatcctg atgcgtccgt caactaattc aaatgctgtg caaggaaagt tattatgatc
     4261 tgattgatct catcaaatgt cggcgtggcg gagccatctg gcgcggtgaa cgaatgtcct
     4321 ccgcgttgca cggccacgag ctgcgcgggc acgctggctt gcaccagccg gtcatacatc
     4381 aactgtgact gttcaaaggg gacaacagtg tcctggtcac catggatcaa cagaaacggc
     4441 ggatcatcag tcgtcacgtg cgtgactgga cttgcctgca caatattatc ttcaccaaag
     4501 ccgacgcccc tcatcgcttc gatatccgca ttcggaaaac ttcgggtgag atccgtgacg
     4561 ggagccatgg caactactgc ctgcacgcgg ctggattgat cgaggtattc acccacgtcc
     4621 cagcctgcgt tgacgtctga tgttccaagc aatgagacca ggtgcccgcc tgcgctgggt
     4681 ccgatggcgg cgatacgatt tggattgagg ttgtattcgc tggcgtgggc tcgcaggaat
     4741 cggatggcgc atttgacgtc ttcgatcatg tttgggaatt tcccttcagg atacaaacgg
     4801 taattgatcg aagccacaag atagccttcg gatgtcattc gatcaacaaa caggagtgct
     4861 tcggtcttgt cgccgtgcat ccagctgccg ccatgaacat acgccaaaac cggccagggg
     4921 ccacccgaat ctgggaagta aagatccagt ttctgaagag acgcgcccgc cgtgcaatag
     4981 ggaaggtcag ctactgcttc gccatacaat ggaaacggaa ctgcacttgg cgtagcagtt
     5041 gttgtttgcg aagcagtcgg ttgtggggtt gcggtgggag agttttgtcc aagcgaacaa
     5101 gctaatgagg gtaatatgaa agcaatcggc aaaacaaatt ttctcacttt tcgacgggtc
     5161 gtaatcaggg tactccttgc aattcaaaat aagggcactt tatgccgctc cgggattgaa
     5221 tgaaattgta ccttcgtaaa gagctatgta gatattacta acccccaact aattttggaa
     5281 atcaacagcc aactacaacg gaggccaggg gagggcgcgc cggtcgcggg gctgacgggg
     5341 aaaagtccct cgtttgataa ttgtgtcggc cctggcgcgg agtgctgaga tctcgcccgg
     5401 gctgagataa cgctcaagat cggccagcag gcttgggtct acggcgaagc gaagacggga
     5461 gagcaaatcc gctggaatct tttgcccact gaaatcccag agcacagtcc ttagtttgtc
     5521 atcctcatgg aagcaaatcc catggtcaat ggcaaacagt ttgtgcgtgc ctgcctcaaa
     5581 aaagacatga ccacctttcc gatctgcgtt gttgcacaga aggtcaaaga gaacgacagg
     5641 ccggagaagg tcccgatctt cttctgtgaa gttgaagtaa tgatattcgg gatcgtattc
     5701 aataaactgc tgaagcgaac cttcgccatg cgggccctct tttcgaagtg tggtgaacgg
     5761 cacaaagttg aagccaaggc actcactgat cagatatgcg gccacctcgc gttgtgcaag
     5821 cgtgtgactg ggaaagtccc agagaggctg ctcgccaagc gacggcttat aaactgcggg
     5881 ataggttgca tcctgataat taacatcgac aaggaatgtg taattggagc caagcataaa
     5941 ctggcccttg atttcaaaat ctccatgtgt cagtacattt ttaagtttta taaaatcggt
     6001 tgggttcatt tatgaagtag ctggctgtta ccacgtctcg tcaagtttat ccgcgtaaaa
     6061 tcggagggca cgctcatagc tgagaatccc gtcgtctttt gttgtatcaa gcagggaagt
     6121 cagcaggagt cccattgcgt ttgtatgatc tttgagattg tgtagagtca tggcaaggaa
     6181 cactttgtat tcattgctct cagggaaaag ttcgagcgcg cgctggaatg tttctttcgc
     6241 tttttgatat tccccaatac agcgatatgt gctgcccatg cccagcaatg caccttgtaa
     6301 atcttctcca cttaagcctg cctgaaccgc actttcataa aatggaatcg cttcacgctc
     6361 caggccaagc aggtcatgga cccatgcaca ttgaaagttg atctgcgcat cctctggaaa
     6421 ttcagaatgc aattccaaga gaagttcacg ggcttcttcg tgttttccgg tttctcttaa
     6481 atgaatcgca gaggagagtt tgtctcgaag agtatccatc aagttaaagg cagtgcacca
     6541 tggattcgtt tgttctcagc agttcagacg ctcagctttc acgttaatgc atgtgaccgt
     6601 tcttcttcgg gcaaaaatgt ccctctggct ccatgggttg ctggcactgg gggcacgtcg
     6661 ggcgcccgcg cgcaacaatc tccacacccc agcgcgccag catccgcacc tgggtgcgcg
     6721 aggcccaaaa gcggatcacc gccgccgttt cgggatcagc ctcttccgtc aaaatttcct
     6781 tgacgaataa cacgatcagg tcgcgatctt tgtcataacc aagcccgatc tcaccgacgc
     6841 gaaacaacgg atccaccgga gggttgatcc gcataacatc ttcgacgtaa tcaccagaag
     6901 cttcttccac gtccgggttc tgctcattga tctgtgaaag gaattgctca acaccaattg
     6961 acagagaatg caattgtgtc ttttcaatga tcacggtaat ggtgcgctgg tcctgatagg
     7021 cttgcagata gaatacgcgc tggcccgggt taccgattgc atccgcggtg atatggtcac
     7081 atgggtcgac atcaatttca aagcggggca tagatttcct tctttcgagc gagtatacca
     7141 gaccccccac ctgtcctcct tccttttttg tctgaagttt gttagaaaat gggaaggtgc
     7201 caaatactag ccgcgggtgc aagtgagggc ggaggggtta taatttctca ccgaccaaag
     7261 gagaccgtca tgtacgataa gaaaaaatta gatgagatga aagactcact taaaaactgg
     7321 gaagagactt ctctaaagaa agccttgtct tcattacccg aacgcgccga tgaatttatt
     7381 acgacttctt ccgaacccat caaccgactc tacacgccgc tcgatgtagc ggatcaagat
     7441 tacgcatctt cccttggact accaggtgag tatccattta cgcgcggagt ccacccgacg
     7501 ctccatcgca gcaaactctg gaccatgcgc atgttcgcgg gctttggcac ggcagaggaa
     7561 acgaacgcgc gcttcaaata tttgctggac cagggacaga cgggattatc catcgctttt
     7621 gatctggcga cattgatggg ctacgacac
//
LOCUS       DASTJP010000213         5966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_143614, whole genome shotgun sequence.
ACCESSION   DASTJP010000213 DASTJP010000000
VERSION     DASTJP010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143614"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFQ23_06475"
     CDS             <1..552
                     /locus_tag="VFQ23_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET9906264.1"
                     /translation="GTIGGSIVHSDPAAELPVLMTALNARLKARNASEERWIEAKDFF
                     VGMFTTALEPDEILVEIELPFSPPRTGWSFMEVAPRAGDYAMMGVAALVTLDENGKCQ
                     KAKLVYLNAGEGPVEATEAAGSLGTQAIDENLINSAAEHASEKELNPFGNMHASAEFQ
                     RHLAKVLTQKALRLATQRAGELQ"
     gene            549..1025
                     /locus_tag="VFQ23_06480"
     CDS             549..1025
                     /locus_tag="VFQ23_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9906265.1"
                     /translation="MTQSIAITVTVNEQQYSRAVEPRLLLSDFLRHELGLTGTHVGCE
                     HGVCGACTILFDGESARSCLMFAAQADGHSITTVEGLATDQDHLHPLQQAFWEAHGLQ
                     CGYCTPGILMSMIPFLRQNPNPTEEDIRHALSGNLCRCTGYQHIVDAVKLAAEKMK"
     gene            1111..2394
                     /locus_tag="VFQ23_06485"
     CDS             1111..2394
                     /locus_tag="VFQ23_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9906266.1"
                     /translation="MMRYSLIDDLRAGQLTFPDFLAQAEARFQEREPDVLAFIPEEDR
                     FARLQEDTETLVLSYPDLISRPVLFGALTGVKDIFQVEGFTTQAGSRLPHDLLQGKEA
                     RSVTQLKEVGALMLGKTVTTEFAYFSPGPTRNPHNPEHTPGGSSSGSAAAVAAGFCHV
                     ALGTQTIGSIIRPAAFCGVVGFKPTYDRISREGVIPLSPSLDHIGFFTSDVDTGKRVA
                     SVLVDDWKSESIERKPTLGIPEGPYLESASDQALAWFNTICESIVQAGYELRRVRVMD
                     DFQEIRARHDVIMSAEASKVHANWFDKYADLYSVKFTELIRRGQAVSDAQLQDAFAAR
                     DSFREQITKTMNDNNIDLWICPPSVGPAPKGLESTGDPVMCLPWTQIGLPAINLPAGK
                     SEAGLPMGLQLVANWDMDEALLHWATYFEQTVRDL"
     gene            2391..4064
                     /gene="fdrA"
                     /locus_tag="VFQ23_06490"
     CDS             2391..4064
                     /gene="fdrA"
                     /locus_tag="VFQ23_06490"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HET9906267.1"
                     /translation="MTVTKWNVRAGAYYDSVVLMQLQRGLLGLPGIVDAGVVMATQAN
                     RELLASNNLLPDSVTANADDLLIVVKADDESSAEDAIGKVDELLARRKSSTVSEDFRP
                     RSLNGAVKQLPEANWVLISVPGRYAAGVARDALDLGKHVFLYSDNVALEDEIALKKTA
                     REKGLLVMGPDCGTAIINGVGLGFANRVRRGTIGVVGASGTGTQAVTVHVHNLGGGIS
                     HAIGTGGRDLKSDVGAITAHQALDVLARDPETKVIALISKPPSPDVAKGLISAAQMTG
                     KPVVIYFIGYPAPARRIGNLHFAISLSEAAEIAVKLGNQETGTEERTSSETRFLRGLF
                     SGGTLAYETLLGLQASLSPIYSNGPITANQDLKDPLKSEAHSIIDLGDEFFMVGRLHP
                     MIDNDLRIRRMKQEAADPGVGMILFDVVLGEGSHMDPASELVPVIKAIQGSRKDIEFV
                     AMVIGTDEDPQNIQAQIDQLNEAGVTVYRTAAEAVEYISLKFSHHDSNQFPPVDLAQL
                     TQPLSAINIGLESFYESLISQGAQAVHVEWRPPAGGNEKLAALLAKMKK"
     gene            4169..5413
                     /locus_tag="VFQ23_06495"
     CDS             4169..5413
                     /locus_tag="VFQ23_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET9906268.1"
                     /translation="MDIEQANTTAVNRLMDARPILKAVATARDVIPGMKDNLFLHAGP
                     PIEWARMSGPLRGAVIGAMLFEGIAKSESEATSMVERGEVEFDSCHHHGAVGPMAGVT
                     SASMKVYVVENAVHGNKSFSNLNEGYGKVLRYGAYSEDVLKKLHWMNDVLGVALADAL
                     ANSNGIDLRALMGEALHMGDEGHNRNKAGSLLYLKLLSPLIAKVVKDETAESEILQFL
                     GDNALSVLNPVMAACKAMTDAAHGVEGSTIVTTMARNGTDFGIRVSGLGEKQWFTAPA
                     EIPVGLFFSGFSQEDANPDIGDSAITETAGIGGFAMATAPAIVTFVGGTPKDAMNATL
                     EMYEITFGESKFFTMPSLDFRGTPTGIDIRKVVELGITPRINTGIAHKNAGVGQVGAG
                     LVRPPLKIFEDALLAYAEKYDL"
     gene            5618..>5966
                     /locus_tag="VFQ23_06500"
     CDS             5618..>5966
                     /locus_tag="VFQ23_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906269.1"
                     /translation="MKLDGSKATRLTSNTVYDGEPAWSPDGTKIAFTSARDGNADIYI
                     MNSDGSEQTRLTRAASNDGQANWSPDGKRIAFASIEDENSDVFTINLDGSGITQLTIG
                     TAFDGGPTWSPDGG"
BASE COUNT         1463 a         1398 c         1682 g         1423 t
ORIGIN      
        1 ggcaccattg gtggaagcat tgttcattct gacccggcag ccgaattacc tgtattaatg
       61 actgccttga acgcgaggct gaaagccagg aatgcttcgg aggaacgctg gattgaggca
      121 aaagactttt ttgtgggcat gttcaccacg gcgcttgaac cggatgaaat ccttgtcgag
      181 atcgaattgc cctttagccc accgcgaaca ggttggtcct ttatggaagt tgcgccgcgt
      241 gcgggggatt atgcgatgat gggtgttgct gctctggtga cgctggatga aaatggaaaa
      301 tgccagaagg cgaagctggt ttatctgaat gccggtgaag gtcctgttga agcaacggaa
      361 gccgcgggaa gtttgggaac ccaggcaatc gacgagaact taattaattc agcggcagaa
      421 catgccagcg aaaaggaact aaaccccttt ggaaatatgc atgcttcagc cgagtttcaa
      481 cgtcatctgg caaaagtgct gactcaaaaa gcacttaggt tggcaactca gcgcgcagga
      541 gaactgcaat gacccaatcc attgcgatca ccgttactgt caatgaacaa caatattcac
      601 gcgcggtcga gcctcgcttg ctgctgagtg acttcctgcg tcatgaactt gggctgacgg
      661 gaactcatgt cggctgtgaa catggcgttt gtggtgcgtg cacaatttta ttcgatggag
      721 aatcagcccg ttcgtgtttg atgtttgcgg cccaggcaga cggtcatagc attacgacgg
      781 ttgaaggtct ggcaacggac caggatcatt tgcatccgtt acagcaggca ttttgggaag
      841 cgcacggctt gcagtgtggt tattgcacgc caggcatttt gatgtcgatg attccgtttc
      901 tccggcagaa cccgaatccg acggaagaag acattcgcca cgcgctgtca ggtaatctgt
      961 gtcgttgtac gggctatcag catatcgtcg acgcggtgaa gctggcagcc gaaaagatga
     1021 agtaagtcac gatggtcgag tagtggtgct ggtcgttggc accacgtatc gagaccacaa
     1081 gcacaatagg attcagattg gtgactgaag atgatgagat actcattgat agacgatttg
     1141 cgtgcgggac aattaacgtt tccagatttt cttgcgcagg ctgaggcacg cttccaggag
     1201 cgagaacctg atgtcctggc attcattccc gaggaagatc gctttgcgcg tttgcaagaa
     1261 gataccgaaa cccttgtctt aagttatccg gatttgattt cacggcctgt cttatttggt
     1321 gcactgacag gtgtgaaaga tatttttcag gtggaaggtt tcactactca agccggaagc
     1381 cgtttgccgc acgatttact ccagggaaaa gaagcgagaa gcgtcacgca gttgaaagag
     1441 gtgggcgcgt tgatgctcgg aaaaacagtt acaactgaat ttgcgtactt ctctccgggt
     1501 cccacgcgca atccgcataa tccggaacat acgccgggtg gttcaagtag cggatcagcg
     1561 gcggcagtgg ctgctggatt ttgccatgtt gcattgggca cgcaaaccat cggatcgatt
     1621 attcgtccag cagcgttttg tggggtggta ggattcaagc ccacatatga tcgtatttca
     1681 cgcgagggtg tcattcccct atcgccatcg cttgatcata ttggattctt tacaagtgat
     1741 gttgataccg gcaaacgagt tgcaagtgta ttggtggatg actggaaatc agaatctatc
     1801 gaacgaaagc cgactcttgg tataccagag ggtccgtatt tggaatctgc ttcagatcag
     1861 gctctggcct ggttcaatac aatttgcgaa tccatcgtgc aggcgggata cgaactgcgc
     1921 cgggtccgcg tcatggatga ctttcaggaa atccgcgcaa ggcacgatgt cattatgtcc
     1981 gctgaagcct caaaggttca tgcaaactgg tttgataaat atgcagatct gtactccgtc
     2041 aaatttactg aactgatccg gcgcggacag gcagtttcag atgctcagct gcaggatgca
     2101 tttgctgcgc gcgattcgtt tcgcgaacag atcacaaaga ctatgaatga taacaacatt
     2161 gatctgtgga tttgccctcc tagcgttgga cccgcgccga agggattgga gagtacaggt
     2221 gacccggtga tgtgtcttcc gtggacgcag atcggtttgc cggccatcaa tctcccagcc
     2281 gggaaaagcg aggctggttt gccgatgggc ttgcaactcg tggcgaattg ggatatggat
     2341 gaagccttgt tgcattgggc aacgtatttc gaacaaaccg tgagagacct atgaccgtca
     2401 caaagtggaa cgttcgcgcc ggtgcttact atgactcagt tgtcttgatg cagctgcaac
     2461 gcggcctgtt ggggctaccg ggcatcgtag atgcaggggt cgtgatggca acccaggcca
     2521 accgtgagct gctcgcttca aataacctgc tgccggattc ggtgaccgcc aatgctgatg
     2581 atttactgat cgtagtaaaa gcggatgacg aatcttctgc agaggatgca atcggcaaag
     2641 ttgacgagtt actcgcccgc cgaaaatcat ccactgtttc agaagatttt cgtccgcgta
     2701 gtttgaacgg tgcggtgaag caattgccgg aagccaattg ggtcttgatt tccgtcccgg
     2761 gtcggtatgc ggccggtgtt gcgcgggatg ctcttgacct tggcaaacac gtttttctgt
     2821 atagcgataa tgttgcgctt gaggatgaaa ttgccctgaa aaagaccgcg cgtgaaaaag
     2881 gtctgcttgt gatgggaccc gattgcggaa ctgcgatcat caacggcgtt gggctgggct
     2941 ttgcgaaccg cgtccgccgc gggacgatcg gtgtggtggg cgcctccggg accgggaccc
     3001 aggctgtgac tgttcatgtt cataacctcg gcggtgggat ttcgcatgcc atcggtaccg
     3061 gcgggcgcga tctcaaatcg gatgtcggcg cgatcaccgc gcatcaggcg ctggatgtac
     3121 tggcgcgtga cccggagacg aaagtcattg ccttaatttc aaagccgcct tcgccggatg
     3181 ttgccaaagg cttgatctct gcggcacaaa tgacgggcaa accggttgtc atttatttca
     3241 ttggttaccc cgcgcccgcc agacgaatag ggaatctaca ttttgccatc agcttgagcg
     3301 aggcagctga gattgctgtc aaacttggga atcaggaaac tggaactgaa gagaggacct
     3361 catccgaaac ccgcttcctg cgaggtctgt tttcaggcgg aacccttgct tacgagacct
     3421 tgctgggatt acaggcaagt ctttccccga tttattccaa tggtccaatt acagcgaacc
     3481 aagatttgaa agaccctctc aagagtgaag cgcatagcat cattgacctc ggcgatgaat
     3541 tcttcatggt tgggcggttg catccgatga tcgataacga cctgcgcatc cgccgcatga
     3601 aacaggaagc cgccgaccct ggtgtaggca tgatcctgtt cgatgtggtg ttgggggaag
     3661 gctcgcacat ggacccggca agtgagttgg tgccagtgat caaagcaatt caaggctcac
     3721 gaaaagacat cgagtttgtt gccatggtga tcggcacgga cgaagacccg caaaatattc
     3781 aggcacagat tgaccaattg aatgaagcag gcgtgaccgt ttaccgtact gccgccgaag
     3841 cagttgaata tatcagcttg aaatttagtc accacgactc gaatcaattt ccacccgtcg
     3901 atcttgcaca gttgacacag ccactgtctg cgatcaatat tggcttggag tcattttatg
     3961 aaagccttat ttctcaaggt gcgcaggcgg ttcatgtgga gtggcgcccg ccggcaggcg
     4021 ggaatgagaa gttagctgcc ctcctggcaa agatgaagaa ataaacaaaa acgtcatcct
     4081 gagcgtagcg aaggatctct aacacaatga gattagactc ttcggtcgct gcaaacgctc
     4141 cctcagagtg acatatcagg agatcattat ggatattgaa caagcaaaca ccactgctgt
     4201 taatcgcctg atggatgctc gtcccatctt aaaagccgta gcaacagctc gcgatgtgat
     4261 tcccggaatg aaagataacc tgttcctgca tgcgggtccg ccgattgagt gggcacggat
     4321 gtcaggtccg ttgcggggcg cggtcattgg cgcgatgctg ttcgagggca ttgcaaagtc
     4381 agaatctgaa gcaacctcca tggtagaacg aggtgaggtg gagtttgatt cctgtcatca
     4441 ccacggagca gtcggtccca tggccggcgt cacctccgcc tcaatgaaag tctatgtggt
     4501 ggaaaatgcc gtgcatggga acaaatcatt ttccaatttg aatgaaggct atggaaaggt
     4561 tttgcgttac ggtgcctata gcgaagatgt gctaaaaaaa ttacattgga tgaacgatgt
     4621 gctgggtgtg gcgttggccg atgcgctcgc aaattccaat ggaatcgatc tccgcgccct
     4681 catgggagaa gcactgcaca tgggcgacga aggacacaat cgcaacaaag caggttcctt
     4741 gttgtatctg aaattgctct ctccattgat tgcgaaagtg gtgaaagatg aaactgccga
     4801 gtcagaaatt cttcaattcc ttggggataa tgcgttgagt gtactgaacc cggtcatggc
     4861 ggcctgcaag gcaatgacgg atgccgcgca tggcgtggag ggcagcacga ttgtcacgac
     4921 catggcacgc aacggcacag actttggaat tcgagtgagt gggcttggcg agaagcaatg
     4981 gttcactgca ccggctgaaa tcccagtggg tctgttcttt tcgggtttct cacaggagga
     5041 tgccaacccg gatatcggcg atagtgccat cacagagacg gctggcatcg gcggtttcgc
     5101 catggcgacc gccccggcca tcgtcacatt tgtaggtggc actccaaaag atgcgatgaa
     5161 tgccacgctc gagatgtatg agatcacgtt tggggaaagc aaattcttca caatgccctc
     5221 cctggacttt cgcggcacgc cgacagggat cgatatccgc aaagttgttg agcttggaat
     5281 cacgccgcgt atcaacactg gtattgcaca caagaatgcc ggcgtagggc aggtaggggc
     5341 ggggttggta cgaccgccgt tgaaaatatt cgaagatgcg ttactggcgt atgcagaaaa
     5401 gtatgattta taggtgatca ttattaaccg gacaatcctg ctgcctgcaa cgttgaaaaa
     5461 agtttgtgta ccagtatcgc tgtgcatggt gcttctatat tcaactgcct gtgggtcgat
     5521 ttcaaatttc aagacagtcg ctgatattcc cctggaatgt agggaaaaaa gaattgcatt
     5581 cagctcgaac cgcgatggta attttgaaat ttatacgatg aaactagacg gctccaaagc
     5641 gacgcgacta accagcaata cggtctacga cggggaacct gcctggtcac ccgatggaac
     5701 aaaaatagct tttacttcgg cgcgtgatgg caacgctgat atctatatca tgaattcgga
     5761 tggatcggaa caaacgaggc tgacccgtgc tgccagcaat gatgggcaag cgaattggtc
     5821 accggatgga aagagaatcg catttgcttc cattgaagat gaaaattctg atgtcttcac
     5881 tatcaatctg gacggttccg gaatcactca attaaccatt ggaacggcat ttgatggcgg
     5941 cccgacctgg tcgccggatg gcggac
//
LOCUS       DASTJP010000214        10385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_512927, whole genome shotgun sequence.
ACCESSION   DASTJP010000214 DASTJP010000000
VERSION     DASTJP010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512927"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFQ23_06505"
     CDS             complement(<1..732)
                     /locus_tag="VFQ23_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET9906270.1"
                     /translation="MPFKLVYYSQQDSQWKGDRLGFGTQQSDTIGYIGCALTSVAMTL
                     SGHGFTETPRTLNKKLQNVGGFAGAGIRWHAVSQLYPQLRLNSIIKCNDTPAPLAQID
                     AAIAAGQPAIVCVDSTAAPGVLTHYVVLIARKGNDYLMLDPWPYQPDVKKETYLMPRY
                     SQGHPLQRSILQIVLFENVAADGVILMPGQIAPPSSGSSETQPTQPTTPPVSTNTGPQ
                     ARVKDDVTWGLNIRSSEDTSSKANIV"
     gene            complement(779..3721)
                     /locus_tag="VFQ23_06510"
     CDS             complement(779..3721)
                     /locus_tag="VFQ23_06510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9906271.1"
                     /translation="MDVLHSLLKRQLSKFSDEAQRLIEEQPEVIQAINDAYLQFEADR
                     RMLEHSLELTSQELKERNAELSRINRELEMRVKTRTADLSNNEARFRGLFEYAPVSIW
                     EEDFSGVRQFIDDLSKTGVQDFSAHFDQHPEDLAECVRRVKIVNVNRATLEMYQAESK
                     EELLADLSQVLGDESLTSFKEELLSLIRGDLIFESELINNTLLGEKRVVFLRLTVAPG
                     FEQSWAKVFVGISDITERRKAETALAAERDLLQALLDNIPDTIYFKDTESRFTRINRA
                     QSKVLGVATPEDAMGKTDFDFFPDPALANGFYEEEQQIVKTGESLTNRVEFHPTRDGQ
                     PRWFAATKVPIKDDNGNIIGIVGVSRDITALKKAEDALREAEIKYRALVEHLPAIVYL
                     DEYDPTVSAGYRPLYMSPQVEVILGYRPEEFLEDPELWPSLLHPDDRERALSTDARHY
                     QAGEMLRQEYHVVARDGHVVWLSDEAAMIRDEVNGKIFSQGVLLDITARKQAEEALRL
                     SEERFRLISWATKDAVWDWNLSTNQIQWGAGLQKIFHYSAETFQTDAEWWRNHIHPQD
                     RAKVDRTLKQALDGGLEFWSKEYRFQRKDGEYVEIMDRAYILRNEAGNPYRMIGAMMD
                     ITERKQTELALQDANEKMARSLNELEMRNQEIRILNEMSDLLQGSSFESQAHAIIGDA
                     AKRLFPDTFGALYLFNSERTVVNRVTFWGDPPPLSQSFTPNDCWALRRGQTHQLYEDE
                     ESKPLCGHILEFPPAVTFCLPMVAQGEILGVLHLHAKSKDHITESKHQLIYTVAEQAA
                     MALSNLKLREALREQSIRDPLTGLFNRRYMEEVLTQHLRRVTRNLHPLGIIMIDIDHF
                     KLFNDSNGHAAGDALLRELGDFLKRRIRGEDVACRYGGEEFLLIMPEASLEAAYQRAE
                     LLRQGSKGLRVRDADKSLGGITLSLGVAIYPQHGRTMDIILRAADAALYRAKQEGRDR
                     VVVADEGN"
     gene            complement(3747..4031)
                     /locus_tag="VFQ23_06515"
     CDS             complement(3747..4031)
                     /locus_tag="VFQ23_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906272.1"
                     /translation="MREQNAIIERTVNSVSKLFLFEGTCQSRNELWMPEVAAPSSYDG
                     RLLVLRQRSEEEELGGVQVLGSNTVHVGSYSYRESSPFASGQQANFTTKR"
     gene            complement(4182..6968)
                     /locus_tag="VFQ23_06520"
     CDS             complement(4182..6968)
                     /locus_tag="VFQ23_06520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9906273.1"
                     /translation="MNSIDQPQLSKQFIGIAKFCSWIVIGISLFALVGWAFDISAFKI
                     VLPGFQPIKANTAFAFFMAGIALWLSCKVGAKLVTSRVVQICNGVVFLIGLLTLSEYI
                     FSLNLGIDQLLFKDVESSLGSFPGRMAFASAVCMTLIGFASIFIYKGRGKGIDLGQYL
                     SVIVLAISVLALVGYIYDVQALYKLDQFSSMSLHTAVGFALLAFGNLSVRPNQGWMQT
                     LATETAGSVILRRVLPVLFGIPIFFGWLIVLGDQAGLYEAHFGLAMLAVLTGGFLIIT
                     LWLNGRQLNLIDLNRKQAEDELRQLNIELEQRVADRTAELKKMNERIQIELLQREQLS
                     EALMTERDLLQTLMDNIPDTIYFKDTASRFTRINRAQANVLGIDTPEDAIGKTDADFF
                     EPNELAKGFYEEEQQIVKSGKALTNRIEFNPTQDGQARWFSATKVPIKDENGTVIGIV
                     GVSRDTTQQKQSEETLRQSEERFRLMSWATKDALWDWDLRNKQILWGEGLQKMFRYPS
                     ETQQTEPEWKHAHIHPQDREKVDRSMNQALEGGMEFWSKEYRFQRFDETYADVMDRGY
                     ILRDDKGTPYRIIGAMMDITERRQAEATILHQNEMLSSLHQITLDLLRYRELNSLLHA
                     LVELVAKFLDASYAEIMLVDEEMLVIQAATQYPPHLIGERLERDDAVLSWKAMDTREP
                     AVLSDYEKWPHRRTVYDEFRLHAVAAFPILNDNDCLGVLALGRNQAGYEFTHDQIQYG
                     RLFANITALVLNNAQLRDTLREQSIRDGLTGLYNRRYMEETLKQQLSRATRQLHPLGI
                     IMLDIDHFKSINDTHGHATGDELLRELGKLLKNNIRNEDIACRYGGEEFLLIMPDTSL
                     ETAVQRAELLRQSVHGLHVQEGQLLEGITLSLGVAVYPQHGLTTESILNAADAALYRA
                     KQAGRDRVVMADRD"
     gene            7193..7831
                     /locus_tag="VFQ23_06525"
     CDS             7193..7831
                     /locus_tag="VFQ23_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9906274.1"
                     /translation="MMKKEKTPKHFHIQDLETLRAVSDPLRIQIVELLTTQNLTVKQV
                     AEKLGLAPSKLYYHFGALEKLGVIEVAETRMVSNMVEKLYQSNADQLDVDPSLLKFSK
                     EGDNEPFNILISSTIDATREDIIRSLQARQFQLDLGAAEQSRRFIINRVVSQISEERV
                     AEFQERLVQLIQEFEGEDKHTSRKPNLQPYALTVAMYPSFYFDKPSKKEKKK"
     gene            7828..9174
                     /locus_tag="VFQ23_06530"
     CDS             7828..9174
                     /locus_tag="VFQ23_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9906275.1"
                     /translation="MTALTMDQQATDAQPGNMNKFLVIWLGQVVSMLGSGLTAFALGV
                     WIFQKTGQATPFALTILFSNLPRILLLPVAGSLADRWNRRQIMILSDVGNALVTIGVF
                     FLLMFSTLEVWHVYLIATLGSIFAAFQEPAYMASITMLVPKKDLARANGMMQMGQALE
                     MVVTPLIAGLLFVSIGITGILTIDFVTFLFAVGALLLVSIPQPELSDEHKQTGSVLSD
                     ARFGWDYLKARPGLFGLLWYYAMMNFLLNWSGVLTGPMLLSQSSPRTLGIVQMVIGIG
                     MLAGGILSSVWRGPKRRIPAVIGFIALALVGMVIAGLRSNPLVVGIGFFWLMLFIPLA
                     SASSQAVFQSKVAPDIQGRVFSIRGMISRSVMPIAYLTAGPLADRVFGPLMNTGGAWA
                     NTFLGTLLDVGPGRGIGLMFVLSAVIAIGISALVYANPRIRNLEDELPDAVPSPEQ"
     gene            9216..10157
                     /locus_tag="VFQ23_06535"
     CDS             9216..10157
                     /locus_tag="VFQ23_06535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET9906276.1"
                     /translation="MKFGLRNTSFVYPGGTGNIWEDVKTHIQRAERDGFDSFWVMDHF
                     YQLPVHGSEEEPFLDAWTVLPALAAVTSRIRLGAMVSPVGYRNPALLARMGASLDHIS
                     NGRMNFGFGAGGYKPEYQAYGFEFIEKGSVRLAQMKEALQLILGLWSESRFTFHGEYF
                     HVENVILEPKPLQKPHPPILIGGVGPRVTLKMIAEIGDACNLWGPPEEFARERETLKR
                     HCEEVGRDESTIEKTTYDLVVCARTQTELEKKIERLLPIKGVEPWMALVGTPSQLVDI
                     VGEYARVGADHLCMDFAGNDPESYELFVEEVMMKFKN"
     gene            complement(10163..>10385)
                     /locus_tag="VFQ23_06540"
     CDS             complement(10163..>10385)
                     /locus_tag="VFQ23_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9906277.1"
                     /translation="TKIFIHRSYPPAEGDLPATNHIAFAVEDVDAACAALAEQGLTLE
                     VPPKDYYWGRSAYLRDPDGYQVEITKTDK"
BASE COUNT         2491 a         2636 c         2514 g         2744 t
ORIGIN      
        1 cactatattc gccttactgg aagtatcttc actgctgcga atgttgagtc cccaggtcac
       61 gtcgtccttg acgcgggcct gtggacccgt gttcgtcgaa acgggagggg tagtcggctg
      121 agtcggttgt gtttcggaag atccggatga tggaggtgca atctgcccgg gcatcaaaat
      181 gacaccatcg gcagcgacat tttcaaacaa aacaatttgc aagatcgaac gctggagagg
      241 gtgtccctga ctataacgcg gcatgagata cgtttccttt ttcacatccg gttggtatgg
      301 ccaggggtcg agcatcagat aatcattgcc tttgcgtgca atcaaaacca catagtgagt
      361 aagcacgccg ggcgcggccg tgctatccac acatacaatg gcaggctgac ccgcggcgat
      421 cgctgcatcg atttgagcaa gcggcgcggg cgtgtcgttg cacttgataa tggaatttag
      481 ccttagctgc gggtacaact ggctcacggc atgccatcga atgcctgcac ctgcgaaacc
      541 accgacgttt tggagtttct tgttgagagt cctgggggtt tcagtaaacc catgcccgct
      601 tagcgtcatc gccacactgg ttagcgcgca accgatgtaa ccaatcgtgt cagactgctg
      661 tgtgccaaag ccaagccggt cccctttcca ctgagagtcc tgttgggagt agtagacaag
      721 tttaaacggc atgaatcacc tcctgaacgg taggataatt atactcttcg ttatttgatc
      781 agtttccttc atcagccaca acgacccggt ctcgaccttc ttgcttggca cgatagagtg
      841 cggcgtcggc ggcccgaagg atgatatcca tagtgcggcc gtgttgtgga taaatggcga
      901 ctcccagaga aagggtgatg ccacctaagg acttgtcggc atctctcaca cgtagtccct
      961 tcgatccctg ccgtaaaagc tctgcgcgtt gatacgcagc ctccagggat gcctctggca
     1021 tgatcaagag gaattcttcg ccgccataac ggcacgctac atcttcgccg cggatccgcc
     1081 ttttaagaaa atcgcccagt tcgcgcagga gcgcatcgcc agccgcgtga ccgtttgagt
     1141 cgttgaacag tttgaagtgg tcaatgtcga tcatgataat gccaagcgga tgcaggttac
     1201 gtgtgacgcg acgcaggtgc tgtgtgagca cttcttccat gtatcggcgg ttaaataatc
     1261 cggtgagtgg atcgcgaatg gattgctcgc gcaatgcctc gcgtaacttc aaattggata
     1321 acgccatcgc tgcctgttca gcgaccgtat agatcaattg gtgttttgat tcggtgatat
     1381 ggtcttttga ttttgcatgc aaatgtaata cgccgagaat ttctccttgt gcgaccatgg
     1441 gtaaacaaaa tgtgacagct ggtgggaatt ccaaaatatg tccgcagaga ggcttggatt
     1501 cttcgtcttc atataattga tgagtctgac cgcgccgcag tgcccagcag tcattgggtg
     1561 tgaagctttg tgaaagcggc ggtgggtcgc cccaaaatgt aaccctatta actactgtcc
     1621 gttcagagtt gaaaagatat aacgcaccga acgtgtcggg gaataatcgt ttcgctgcat
     1681 cgccgataat agcatgcgct tggctttcaa aactgctgcc ctggagcaaa tcgctcattt
     1741 cattgagaat gcgaatttcc tggttgcgca tttccaattc atttaatgag cgtgccattt
     1801 tctcatttgc gtcctgtaaa gcgagctctg tctgtttgcg ctccgtaatg tccatcatgg
     1861 cgccgatcat gcgataggga ttgcctgcct cattacgaag aatgtaggct cgatccataa
     1921 tttctacata ctctccatct tttcgttgaa atcgatattc ctttgaccag aactccaacc
     1981 ctccatctaa ggcctgcttt aaggttcgat ccaccttggc tcgatcctgt ggatgaatat
     2041 gattacgcca ccattccgca tctgtctgga acgtttcagc cgaataatga aaaatttttt
     2101 ggagccccgc gccccactgg atttgattcg ttgacaagtt ccaatcccaa acggcatcct
     2161 tcgtggccca cgagatgagt ctgaaacgct cctcgctcag acgcagagct tcttccgcct
     2221 gtttgcgcgc cgtgatatca agcagaacac cctggctaaa tattttgcca tttacttcat
     2281 cgcgaatcat cgctgcctca tcacttagcc agacgacatg tccatcgcgc gcaacgacat
     2341 gatattcctg acgtagcatt tctccggcct ggtaatgccg ggcatcggtg gaaagtgcgc
     2401 gctcgcgatc atcagggtga agcagagagg gccaaagttc ggggtcttca agaaattctt
     2461 ccggccggta ccctaagatg acctctacct gtggactcat atacaatggg cgatatccgg
     2521 cagagacagt gggatcgtat tcatccagat agacaatcgc gggcagatgt tccaccagcg
     2581 cccggtattt gatttctgcc tcccgcaaag catcttcagc tttcttgagc gccgtgatat
     2641 cacgcgagac accaacgatc cctataatat ttccattgtc atctttgatt gggaccttgg
     2701 tggcggcgaa ccagcgcggc tgcccatccc gggtcgggtg gaattcaaca cggttggtta
     2761 atgattcccc cgtttttaca atttgctgtt cttcctcata aaacccattg gcgagcgcgg
     2821 gatccggaaa aaaatcaaag tccgtctttc ccatcgcgtc ttccggcgtt gctacaccca
     2881 gcaccttgct ctgagcacgg ttaatgcggg taaagcgcga ctcggtgtct ttgaaataga
     2941 tggtatccgg gatattatcc aggagcgcct gaaggagatc gcgttcagcg gctaaagcag
     3001 tttctgcctt tctgcgctct gtaatatcac taattccgac aaagaccttt gcccaggatt
     3061 gctcgaagcc aggagctacg gtgaggcgta agaaaacaac ccgtttttca cccagtaatg
     3121 tgttattgat cagctcagac tcgaaaatca agtcgccgcg gatcagcgag agcaattcct
     3181 ccttgaacga agtcagagat tcatccccca ggacctgcga caaatctgcc agaagttctt
     3241 ctttactttc tgcctgatac atttcgagtg tggcacgatt tacgttcacg atttttacgc
     3301 gtcgcacaca ttcagcaagg tcttccggat gttgatcgaa gtgagcagaa aaatcctgta
     3361 ccccggtttt gctcaggtca tctataaatt gccgtacacc agaaaaatcc tcctcccaaa
     3421 tcgaaacggg ggcatattca aacaaaccac ggaaacgtgc ctcgttgttg cttaagtctg
     3481 ctgtacgcgt cttgacgcgc atttcaagtt ctcgattgat gcggctgagt tccgcatttc
     3541 tctccttcaa ttcctgcgag gttagttcca ggctgtgttc gagcatcctt cgatctgcct
     3601 caaattgcag ataggcatcg ttgatcgctt gtataacttc gggctgctcc tcgatcaatc
     3661 gttgggcttc gtctgagaat tttgaaagtt gccgctttaa cagactgtgt agaacatcca
     3721 ttacacctcc gataaagtgc tgattgtcat cgcttggttg tgaagttcgc ctgttgcccg
     3781 gaagcaaatg gcgaactttc acggtaggaa taagagccaa catgaacagt gtttgaccct
     3841 aacacttgca cacctcccaa ttcctcctcc tcactgcgtt gtctgagaac tagtagacgt
     3901 ccatcgtaac tgctcggcgc cgctacttcg ggcatccaaa gttcattccg gctctggcag
     3961 gttccttcaa aaagaaataa ttttgaaacg gagttcacgg tacgttcgat tattgcgttc
     4021 tgttctctca tcagattcat cttccaccgc accagcggaa taaaagcaat ccatacaatg
     4081 aaacacccca ctcagaaaac ataaagtttt gtcaacgtgc cacacctgat gattataatc
     4141 tgtacatctt atactttctt atcccaaagg taaggtgtag tttagtccct gtctgccatc
     4201 acgactcgat cacgacctgc ctgctttgcg cgatagagcg ctgcgtcggc cgcattcagg
     4261 atcgattcag tcgttaatcc atgttgtggg tatacggcga ctcccaaaga taatgtaatt
     4321 ccctccagaa gttgaccttc ctgcacgtgt aagccgtgca cagactgccg caagagctcc
     4381 gcgcgctgga ccgcagtttc caatgaggta tctggcatga tcaacaggaa ctcctcgcct
     4441 ccataccgac atgcaatatc ctcattgcga atattatttt tcaatagttt ccctaattcg
     4501 cgtaagagtt catcccctgt tgcatgtcca tgggtatcat tgatactctt aaagtgatcg
     4561 atatccagca tgatgattcc caaggggtga agctggcggg ttgcgcggct gagctgttgc
     4621 ttcagcgttt cctccatgta acggcgatta tataacccgg tcaacccatc gcgaatggat
     4681 tgctcacgga gtgtatcgcg cagttgcgca ttattgagca ccagcgccgt gatgttggca
     4741 aacaggcgtc catattgaat ttgatcatgc gtgaattcat aacctgcctg attccgcccc
     4801 agagccaaca cgcccaggca atcattatcg ttaaggatgg gaaaagccgc cacggcatgg
     4861 aggcggaact catcataaac agttcgccgg tggggccatt tttcgtagtc acttaacacc
     4921 gctggctcac gcgtatccat tgccttccag gaaagtactg cgtcatcccg ttccagccgc
     4981 tctccgatca gatgcggtgg atattgagtt gcggcctgaa tcaccagcat ctcttcatct
     5041 accagcatga tctccgcata tgaagcatcc aaaaatttcg ccactagttc taccagtgca
     5101 tgcaagagtg aattgagttc gcgatatcta agcagatcga gcgtaatctg atgaaggctg
     5161 gaaagcattt cgttttggtg caggatggtc gcttcggcct gtctgcgttc agtgatgtcc
     5221 atcatcgcac cgatgatgcg atatggggta cccttatcat cccgcaggat gtatccgcga
     5281 tccatcacgt ctgcatacgt ctcatcgaag cgttgaaagc gatattcctt cgaccagaat
     5341 tccatgccac cctccagagc ctgattcatg gaacgatcta ctttttctct atcctgcggg
     5401 tgaatatgag catgtttcca ttcaggttca gtctgctgag tctcagatgg gtaacgaaac
     5461 atcttttgta gaccttctcc ccacaagatc tgcttgtttc gcaaatccca atcccagagc
     5521 gcatctttgg ttgcccacga catgagcctg aagcgctcct cgctctggcg gagtgtctct
     5581 tcggattgtt tttgttgagt ggtgtcacgc gaaacaccca caatcccaat taccgtcccg
     5641 ttctcatctt taataggaac cttcgtcgcg gaaaaccagc gcgcctgccc atcctgggtc
     5701 gggttgaatt cgatacggtt ggttaacgct tttccgcttt ttacgatttg ttgttcttcc
     5761 tcataaaaac cttttgcaag ttcatttggt tcaaaaaaat cagcatctgt tttgccaatt
     5821 gcatcctcag gtgtatctat tcctaacacg ttcgcttgag cacggttgat gcgggtaaag
     5881 cgtgaagccg tatctttgaa gtaaatggta tcggggatgt tatccatcag cgtttgcagc
     5941 aggtcccgct cggtcatcag tgcctctgat aactgttcgc gctggagcag ctcgatttgt
     6001 attcgttcat tcatcttttt caattccgcc gtgcggtctg ccacgcgttg ctctaattca
     6061 atattgagtt gtcgtagttc atcctcggct tgttttctat ttagatcaat gagatttaac
     6121 tgcctgccat ttaaccaaag ggtaataatt aaaaagcctc cggttaatac tgcgagcatt
     6181 gccagcccaa aatgggcttc atataaccca gcctgatcgc ctaaaacgat gagccagccg
     6241 aagaagatgg gtattccaaa aagaacaggg agcactcgcc tcaggatgac gctacccgct
     6301 gtttccgtcg caagagtctg catccacccc tgatttggcc gaacacttaa gttaccaaag
     6361 gcaagcaggg caaaacctac tgccgtatga agcgacatgg aggaaaattg atccagtttg
     6421 tacaaggcct gaacatcata aatgtagccc accagtgcta acaccgaaat agccaatacg
     6481 atcacactca gatattgccc gagatcaatg cctttaccac gtcccttata aatgaatata
     6541 gatgcaaatc caatcagagt catacaaacc gcactcgcga acgccatccg cccaggaaat
     6601 gatccaagcg aggactctac atccttgaag agcaattgat caatccccag attgaggcta
     6661 aaaatatatt cactcagggt aagtaatccg atgaggaaca ccactccatt gcaaatttga
     6721 acaactcgcg aagtgacgag tttggcgcca actttacatg aaagccacag cgcgatcccg
     6781 gccatgaaaa aagcaaaggc agtgttggct ttgataggct ggaatccggg tagtacgatt
     6841 ttgaaagcgg aaatgtcgaa cgcccagcct accaacgcaa ataggctaat gcctatcacg
     6901 atccaactgc agaatttcgc aatgcctata aattgttttg ataattgcgg ttgatcgatt
     6961 gagttcacag gtcaccttga tcctaaccat gtgacagacg acgaaatccc ccaagaagta
     7021 caacacaaac tttgttagtg tacaacacag tcaaattata acgagattcc gttggtagag
     7081 ggcgtacctg ctgcaaatca caggaattca gccaaaaccc ttgacaaggg tcagatttat
     7141 tgtaaaatgt tgtccatcgt taaaatttgt ttttatgata agtaggattt ttatgatgaa
     7201 aaaagaaaaa acgcctaaac attttcatat tcaagatttg gagactctgc gggcggtttc
     7261 tgacccatta cgcattcaga ttgtggaact gctcaccact cagaacctca ccgtcaaaca
     7321 ggtcgctgaa aagctagggc ttgctcccag caaactgtac taccactttg gcgccctgga
     7381 gaaactcggc gtgatcgaag tagccgaaac acgtatggtc agcaacatgg tcgaaaagct
     7441 ctatcaatcc aatgcggatc aattggatgt ggacccttcc ctgctcaagt tttcgaagga
     7501 aggcgacaac gaaccattca atattctgat ttcatccacg atcgacgcga cgcgcgaaga
     7561 catcatccgc agcttgcagg cacgccagtt ccaactggac cttggagccg ccgaacaatc
     7621 gcgtcgtttc attatcaatc gcgttgtcag ccaaatctct gaggagcgcg tcgcggaatt
     7681 ccaggaaaga ttggttcaac tcattcagga atttgagggc gaggataagc acacatcccg
     7741 caaaccgaac ctgcagcctt atgcattgac tgtggcaatg tacccgagct tttattttga
     7801 caaaccatca aagaaggaga agaagaaatg accgctctca caatggatca gcaagctaca
     7861 gatgcccagc cgggcaatat gaacaaattc ctggttattt ggctcgggca ggtagtttcc
     7921 atgctcggtt ccggtctgac ggcttttgct ctgggcgtat ggatctttca aaaaactggt
     7981 caggcgactc ctttcgcatt gaccatttta ttttcgaatc tgcctcgcat ccttcttctt
     8041 cccgtggctg gctcccttgc agacagatgg aatcgtcgcc aaattatgat tctctccgat
     8101 gtcggaaatg cgctcgtgac aataggcgta ttcttcctgc tgatgttttc cactcttgaa
     8161 gtttggcatg tttatctgat cgcgacattg ggatcaatct ttgcggcatt ccaggaaccg
     8221 gcctacatgg cctctattac catgctggtc ccgaaaaagg atcttgcgcg cgcgaacggc
     8281 atgatgcaaa tgggacaagc tcttgaaatg gtggtcacac ctctcattgc aggtttgctt
     8341 tttgtttcca tcggcatcac cggcattcta acaattgact ttgttacgtt tctatttgcc
     8401 gtgggtgcgt tattgcttgt gtccattcct cagccagaac tcagcgatga acataaacaa
     8461 acgggctcag tgctatctga cgcacgcttt ggctgggatt atttgaaagc gcgtcccggg
     8521 ctctttggtc tattgtggta ttacgccatg atgaacttcc tcctcaactg gagcggagtc
     8581 ctgaccggac cgatgctgct ctcgcaatcc tcccctcgca cacttggtat tgtacaaatg
     8641 gttattggaa tcggtatgct ggcaggtgga atcctgagta gtgtatggcg tgggccaaaa
     8701 cgacgtatcc ctgctgtcat cggtttcatt gcgttggcgc tggttggcat ggtcattgcc
     8761 gggctacgct caaatcctct tgtggtcggc attgggtttt tctggctgat gctcttcatt
     8821 cctttagcct ctgcctccag ccaggcagtc tttcaatcga aggtcgcgcc ggatatacag
     8881 ggacgagtct tctcgatccg cggcatgatc tcgcgctcag tgatgcccat tgcctatctc
     8941 actgctggtc cgttggctga tcgggtgttt ggtccgctta tgaatacggg cggcgcatgg
     9001 gcaaatacgt tcctgggaac cttgttggat gtcggcccag ggcgtggcat tgggttgatg
     9061 ttcgtgcttt cggctgtgat cgccattggc atcagtgcgc tggtttatgc taatcctcgc
     9121 atccgcaatc tcgaggacga actgccggat gcggttcctt ctccagaaca atagacagtt
     9181 ccgcaaagac tattacttcc aggatcatcc tattcatgaa atttggtctt cgcaatactt
     9241 catttgttta tcccggtggc acagggaata tctgggagga tgtcaaaacc cacatccagc
     9301 gcgcggaaag agatggcttt gactcgttct gggtgatgga tcacttctat caattgcccg
     9361 tgcatggttc agaggaggaa ccgttcctcg acgcgtggac cgttctccct gcgctggcgg
     9421 cggtgacgag tcgaatccgt ttaggtgcca tggtcagccc ggttggatat cgcaatcccg
     9481 cgctactagc gcgcatgggt gccagcctgg accatatcag caatgggcgc atgaattttg
     9541 gttttggcgc aggaggctat aagccggagt atcaggcata tggttttgag tttattgaaa
     9601 aaggttctgt tcgtttggca caaatgaaag aggcgctgca gcttattctt gggttatggt
     9661 cagagtcgcg ttttactttc catggagaat actttcatgt ggaaaatgta atccttgaac
     9721 ccaagccttt gcagaagccg catccaccaa ttttgatcgg cggtgttgga cccagggtta
     9781 cgttgaagat gatcgctgaa atcggagacg cctgcaattt atggggtccg cccgaggaat
     9841 tcgcgcgcga gagagaaacc ctcaaacgtc attgtgagga ggtcgggcgg gatgagtcaa
     9901 cgattgaaaa gacgacctat gacctggtcg tctgcgcgcg cacacagact gagttggaaa
     9961 agaaaattga gcggctgctg cctataaagg gcgtggaacc gtggatggcg ctggttggca
    10021 caccatctca attggtagat attgttggag aatacgcgcg cgtgggtgcg gatcatctct
    10081 gtatggattt cgcggggaat gaccccgagt cgtatgaatt gtttgtagaa gaagtgatga
    10141 tgaagtttaa aaattagctt gcttatttat ctgtcttcgt gatttctacc tgataaccat
    10201 ccgggtcacg taaataagca gaccgacccc aataataatc cttcggtggg acttcgagag
    10261 tcaatccctg ttcggcgaga gccgcgcacg ctgcatccac atcctctacc gcgaacgcaa
    10321 tatggttggt cgctggcagg tcaccttcag cgggaggata gctgcgatga ataaaaattt
    10381 tcgtt
//
LOCUS       DASTJP010000215        14369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_956755, whole genome shotgun sequence.
ACCESSION   DASTJP010000215 DASTJP010000000
VERSION     DASTJP010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956755"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFQ23_06545"
     CDS             complement(<1..427)
                     /locus_tag="VFQ23_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET9906278.1"
                     /translation="MTQWRYDRLTWPEMKDVIARDPQPVVCIPFGTVEDHGTHLPIST
                     DNDILEAVLSECGRRAEGDLLITPTIPVGLDEHHMDFPGTLSVDMETCLAYVSQVAIS
                     VARHGFTHIFIVNGHGSNQSICELAARKVVLETGVICANT"
     gene            complement(532..1371)
                     /locus_tag="VFQ23_06550"
     CDS             complement(532..1371)
                     /locus_tag="VFQ23_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET9906279.1"
                     /translation="MATIINWFITPLQYEFMQRGMIAAILVGIVCAVVGTYVVLRGMA
                     FFGDALAHTILPGIALGYLVSGGAREPLFWWALGTAVIAALGIGAITKNAEIKEDTAI
                     GIIFAGMFALGIALISTVRSYTVDLSHFLFGDVLSVSTQSLWLILIFGGLVILIVLAF
                     YKEFTTLSFDPILAITLRLPVGVLNNLLLALIAVTVAVSLQTVGVALMVAMLVTPAAT
                     AFLLTKRLSTMMIIAAFIASGSGVIGLYLSFYLSIASGAAIVLTATLFFIITFVWKRI
                     KQK"
     gene            complement(1355..2164)
                     /locus_tag="VFQ23_06555"
     CDS             complement(1355..2164)
                     /locus_tag="VFQ23_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET9906280.1"
                     /translation="MYPHTHHQTNQPILDVQHLTVRYNGNIGLEDITFHLHEGERVAV
                     VGPNGAGKSTLFKVVSGVIAPTTGEVNIYGSKPRGHVCIAYIPQRSQVDWNFPLSVAD
                     VVMMGRSAKLGPLSWPRKKDWEFVHHALDTVELSNLTTRQIGQLSGGQQQRMFIARAL
                     AQEAELMLMDEPLTGLDTPAQEGILSLLDRLKKENVTVMVATHDLEQAANYFDRIMLL
                     NHKMIAFDEPKQVLRTENLVKAYGGRLHSTHNESILTVDDCCDDGDHGHNH"
     gene            complement(2167..3066)
                     /locus_tag="VFQ23_06560"
     CDS             complement(2167..3066)
                     /locus_tag="VFQ23_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET9906281.1"
                     /translation="MKQLFSFLLVIALISACGGRSTSPSESTKPVVLTSTTFLADIAQ
                     NIAGDRVDVESLLPVGADPHSYQATPQDVTKIADSKLLFINGAGYEYFLESLLENAGG
                     EREVIEASAGLHPRADVESEQGGTDPHLWLDPNNVITYVENIREGLTHYDPEGAAVFQ
                     SNAEAYIAQLTELDSWISEQVSQIPPERRLLVTNHESLGYFAERYGFRVIGAVLPSAS
                     SDAAPSAEQMARLIDQIKASGTPVIFLDEVENLALARQIADETDTKVVEDLHLESLTD
                     GPPAGTYIDMIKHNITRIVNALK"
     gene            complement(3063..3479)
                     /locus_tag="VFQ23_06565"
     CDS             complement(3063..3479)
                     /locus_tag="VFQ23_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET9906282.1"
                     /translation="MNEHPWLTQLNERGYRLTGARRAVVETMAQSIHALTPIEVYDAA
                     REAYPALGLVTVYRTLEALEEQGLIQRVHQPRGCQSFITAPAGHQHLLLCKECGQTLT
                     FEGDDLESLIKSIARRTGYQINEHWLQLFGLCPECK"
     gene            complement(3745..4299)
                     /locus_tag="VFQ23_06570"
     CDS             complement(3745..4299)
                     /locus_tag="VFQ23_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906283.1"
                     /translation="MNSQIKNLVAMRDVESLYEIMTEDDEWINQLDAAEGLVKLGDRR
                     GYEFLITATMSDDDSILEVANEILASPELARMRAEIEAERDRERRTRVETARLRLQKG
                     GKVFRYKMVYLAAGAILSDDPLNEGFDVPALENHGLEGWEVINIIPRRRTMLVGGVDD
                     HFTGAYFLLKKEVLPHESVDLDQE"
     gene            4450..5577
                     /locus_tag="VFQ23_06575"
     CDS             4450..5577
                     /locus_tag="VFQ23_06575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9906284.1"
                     /translation="MFRGFFSQILVDLGDMEGDYRVFHLKNDIAQSILSLSIASLSIL
                     GMLRMDSWYLRNDPNLLMVLMLYRGGFVIATVLVVLAITRIRKVRIYDRLLLGWVFCA
                     ILFFVLSNFTRPTNYLSTASDIIVPFAIYVLSPFRVLYSIALAFGFSMGTLYVDHFFK
                     TGVDPIILSAATSAQIVVHALGLGSALQIQSYRRRSFKAYIKEKDAKEMVSYLANIDP
                     LTKSMTRRQFFNIAETEFLRFSRYRRPFSVLALDLDRFKDVNDTHGHHAGDLVLRSFS
                     LVALEQKRAQDTFGRLGGEEFGLLLPETNLEQAKVVADRIQKTWEQSPVNLDGVPIYS
                     TVSIGVAEANSEDKSLDDLLRRADQMLYKAKERGKNQVAVE"
     gene            complement(5784..6191)
                     /locus_tag="VFQ23_06580"
     CDS             complement(5784..6191)
                     /locus_tag="VFQ23_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9906285.1"
                     /translation="MEIQVSIHEANEPVAVMNIKGNVDAANYVEVVNKAQEIYNNPVR
                     NLILDLSEVPYISSAGLVAIHKIALIYSGGKQEVEQEGKETRPDFTHHANARKRVKLL
                     NPQPDVDRTLETTGLKLFFKVYKDLDSALKSFQ"
     gene            complement(6211..7491)
                     /locus_tag="VFQ23_06585"
     CDS             complement(6211..7491)
                     /locus_tag="VFQ23_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906286.1"
                     /translation="MKRSNLGNWAIIGLVILNVVVWVVFPPVNDGEPNFIRRYVGEVI
                     GSNNIVLMAISLFLSTRPKWAEPYFGGLDKMYMTHRHTGTAAFLLIFAHVLTVPITTT
                     GWLLGNYLGVIAFTGIVTIVLITLAPRIPFLNKLSGGDYEDWKKLKRYIGIFFILGFF
                     HSLTVKALDAKIAITWVMIFFIIGTVSYLYTEFFGGIFKQYRPYQVDAVKHPNNSSTE
                     VTLRPKKDPMKKHRAGQFLFVRFPKDKVLNESHPFTISSAPSEDVLRLTIKASGNFTR
                     DLFSHLKEGTDATIEGPYGMFNYKTGGPKQIWIAGGIGLTPFLSFVRDMDNSLAQDVD
                     LYYTVRHPEEAIFVDELEAADTKNSRLRVHIRYSAKDGSLRIEDIVKNAQGDIRSYHV
                     YMCGPLPMTMAFEKKFLELGVPRENLHYEEFNFR"
     gene            complement(7635..8045)
                     /locus_tag="VFQ23_06590"
     CDS             complement(7635..8045)
                     /locus_tag="VFQ23_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9906287.1"
                     /translation="MNISVSKEAGRVDVTALRVEGQLDGQSYQSLITKARELFDAGAH
                     DFLIDLTDLTYISSAGLVALHSIALMLKGEEMPDTEHGWSAYRSMGRGSEAGVQAHIK
                     LLNPSEEVRSVLEMVGFDKVFEIFTDRTEAVKSF"
     gene            complement(8145..9425)
                     /locus_tag="VFQ23_06595"
     CDS             complement(8145..9425)
                     /locus_tag="VFQ23_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET9906288.1"
                     /translation="MSSGNPARNSSLADRLKALGVKVGTADLAPPEPKPHYGIDSVVA
                     GAFHPTALGDVFVAEQDYTIDYRHGLSSLTCSLPISVISQWARDARIAELPLSEFAFL
                     DTETSGLSGGTGTYAFLVGVARFVDDQFLLRQFFMRDPAEEPALLEGLANFLAPCKAL
                     VTFNGKAFDAPLLTTRYSLHRIPVPYRDYSHLDLLPLARRLWRDRLPSRALKYLEENI
                     LGLARSTEEVPGYEIPWLYFDYLRTGDARPLAGVFYHNAMDVVAMAALLSHMNNMLET
                     PYDGHVQHGLDFIALGKLFEDLGYWDEAARLFERGLESGAEAESDLTASDFGVAVKRL
                     AILQKKRGDMNEALRLWEDAAERGHLYAFLELAKHHEHKSRDVKTAIKWTKLAMKCAK
                     RTDMPEYMRKHWQDEITHRLERLQRKHDDKKKSV"
     gene            complement(9521..10180)
                     /locus_tag="VFQ23_06600"
     CDS             complement(9521..10180)
                     /locus_tag="VFQ23_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906289.1"
                     /translation="MAFKSQIQRIVLSRGLLFFIFISTFLNHGAVSAMENNNVSLPNF
                     TEFSQAVRNGTSNLLGGVYVPNVLALPVVQQPAGNFGYVSTNDGQVTQFDMASRYGNV
                     GLLAHNYLSGKEFSHLAVGQEVRLVYGDGKVEYFVVKEILKYQALQPNSPWSSFRDLK
                     DDTVLTAEQMFNRVYAGEHHVTFQTCIYAEGISSWGRLFVVAVPKTDFSADELWWGPV
                     Y"
     regulatory      complement(10242..10341)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      complement(10417..10509)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(10606..11259)
                     /locus_tag="VFQ23_06605"
     CDS             complement(10606..11259)
                     /locus_tag="VFQ23_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906290.1"
                     /translation="MALKNRFARMIFGGGMLTGLVLVFFTPIFNVGTGNGEPTPADFL
                     DFIQQVQNGNVDVLSGVYIPSVLALPVVQQPTGDDGYVSGRNDEATQFSMASRYGNIG
                     LLAHNYLSGRLFSQLAVGEEVRLIYGDGRVEYFVITEVLRYQALEPNSPWSSFRNLES
                     EDILSTEQMFTRAYAGDRHVTFQTCIESNGNASWGRLFVIAVPKALSFETIFQQLLQ"
     regulatory      complement(11321..11413)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11556..13001
                     /locus_tag="VFQ23_06610"
     CDS             11556..13001
                     /locus_tag="VFQ23_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9906291.1"
                     /translation="MTTESRERLPVWVKLLYGSGDWGIASIGIMRSIFYAIYLTDVVG
                     LEPRIASFGAIVGIIWDAINDPIIGTFSDRLNTRWGRRRPFLLWFAIPFALSFVILWS
                     APQWENQIALLIYVTLSFMLADTLQTLISVPFLSLTPELTPDYDERTSLTSFRSFFQL
                     VGALTVVIAAPIIVDLIIESGGSQQQGFMLVGAIFGSISAVPLLLIGLFLRESSKPDE
                     IKSIPFKETLRLAWGNVPFRYAVGIHMLNWSAVDMIAVAFPYFLLYWVAQGDLLAKIR
                     IFGIDLAYESAFFGILMSVCILFIPFWLWLAKKRNKREAYILGMAFWIIVEFMVFTIQ
                     PGQTNYLIAIAALAGIGVSAAYTLPDAMFADVIEWDELRTRRRQEGIYYGVRAFIRKV
                     TGALMVFITLQALGWSGYVSPPESATQFTQGDTALQMIRLLVSPFGALMLCGTIIFAW
                     LFPLSRETHGRIQKLLEKRRARIIKVETPSP"
     gene            complement(13017..13490)
                     /locus_tag="VFQ23_06615"
     CDS             complement(13017..13490)
                     /locus_tag="VFQ23_06615"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HET9906292.1"
                     /translation="MNTEFLLVVGIIVFVLIILNAIFLGIIFFLRRKMAAVSQWPSTM
                     GTVNSSYLERRSSSEGGSTNYPVVQYSYQIGGQSYQSSKLAPGPDVGGTGAGKVVARY
                     PAGAQVMVFYNPQNPADAVLERKAPAQWLMWLLLIVFDCALCGVLPILYFTMGQR"
     gene            complement(13570..>14369)
                     /locus_tag="VFQ23_06620"
     CDS             complement(13570..>14369)
                     /locus_tag="VFQ23_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906293.1"
                     /translation="GRIAAVKLGQTDDRLDAWAIGYSPSRVAVVWTGARQEGALSPRV
                     PAVLWNALMQTASQNLPGDGWAVPADVSVINVCDPSGMLPTTDCPNLVSEVFLNGNEP
                     VQPDAMYRRFAINRETGFLATVFTLPQLVEDRVYFVVPADARSWAESAGVEIPPTTYD
                     VIQSPQINPDVNITSPELFAEVTGEVRISGTAAGENFVSYRLLVGQGLNPQEWIQVAE
                     NAGPVTDGLLAEWNTTGLSGLYAVQLQVVRSDQRVDSAVIQVTVVMP"
BASE COUNT         3705 a         3661 c         3273 g         3730 t
ORIGIN      
        1 tggtgtttgc acaaatgact cccgtttcaa gcacaacctt acgcgctgcc aactcgcaga
       61 tcgattgatt cgacccatgc ccattgacaa taaagatgtg cgtgaaccca tgccttgcca
      121 cagaaattgc gacctgtgat acataagcga gacaggtttc catgtctacc gacaacgtac
      181 cggggaagtc catgtgatgt tcatccaaac ccacagggat cgtaggtgtg attagcaagt
      241 caccttctgc ccgccgccca cactcggata acaccgcttc gagaatatca ttgtcggtcg
      301 agatcggaag gtgtgttcca tggtcctcca ctgtgccaaa cgggatgcaa acaactggtt
      361 gaggatctcg ggcgatcacg tctttcattt caggccaggt caaacggtca taacgccatt
      421 gtgtcatgta tgcctccaag gtttttcctt atttaaacac aaagttcaca aagattttaa
      481 gtattttctc tgtgtctccg tattctgatc ggcaatgtat aaaaacctga gttatttttg
      541 tttgattcgt ttccacacaa atgtaatgat aaagaatagt gtcgcggtca acacaatcgc
      601 tgcaccagag gcgatgctca aatagaatga aagatacaag ccaatcactc ctgaaccgga
      661 tgcaatgaaa gccgcgataa tcatcatcgt tgataagcgt ttcgtaagca aaaaggcggt
      721 cgccgcgggg gtgacgagca tcgcaaccat caacgctaca ccgaccgttt gaagggagac
      781 cgcgaccgtc acggcgatca gtgccaacag aaggttattc agcacgccca caggtaatcg
      841 aagcgtaatc gccaggatgg ggtcaaaaga tagggtcgtg aattccttat agaacgccaa
      901 aacgatcaga atgacaagtc cgccaaagat gagaatcagc cacaggcttt gcgttgagac
      961 acttagcaca tccccaaata ggaagtgact caaatctacc gtatagctgc gaactgtgga
     1021 gatcagcgcg atacctagtg caaacatccc ggcaaagata atgccgattg ccgtatcttc
     1081 cttgatctct gcgtttttcg tgatggcgcc gatacccaga gcggcaataa ccgccgttcc
     1141 caacgcccac cagaaaagcg gttcacgtgc accaccacta accagatatc caagcgcaat
     1201 gccaggcaaa attgtatgcg ccaaagcgtc cccaaaaaaa gccatcccgc gcagcacaac
     1261 gtatgtgcca acaactgcgc agacaatccc tacaaggatt gcggcgatca tgccccgctg
     1321 cataaattcg tattggagag gcgtgataaa ccagttaatg attgtggcca tggtccccat
     1381 cgtcacagca atcatccacg gttaaaatgc tttcattgtg ggtggaatgt aatcttccgc
     1441 cataggcctt tacaaggttt tcagtccgca acacctgctt tggttcgtca aacgcaatca
     1501 tcttatgatt gagcagcatg atccggtcaa agtaatttgc ggcttgctca agatcatgag
     1561 tcgcgaccat cacagtgaca ttttcctttt tgaggcgatc caacagggaa aggattcctt
     1621 cctgagccgg ggtatccaat ccggtcagtg gttcatccat cagcatcaat tcagcttcct
     1681 gcgcaagcgc gcgggcaatg aacatgcgtt gctgctgccc tccagagagt tgaccgatct
     1741 ggcgcgtggt caggttactt agctctaccg tatccaacgc atggtgaaca aactcccaat
     1801 ccttcttgcg cggccagctg agtgggccca gctttgccga acgtcccatc attacaacat
     1861 cagcaacact caaggggaag ttccaatcca cctgactacg ctgtgggatg taggcaatgc
     1921 acacatgccc gcgtggcttt gagccatata tatttacttc acctgtggta ggagctatga
     1981 ctccggaaac aactttgaag agagtgcttt tccccgcgcc gttgggtcca acaacagcga
     2041 cacgttcacc ttcatggagg tgaaaggtta tatcctcaag cccaatgttg ccgttatagc
     2101 gaacagtgag atgttggaca tccagaatgg gttggttggt ttgatggtga gtatgtggat
     2161 acataattac ttcagtgcgt tcacaatccg cgtgatgtta tgttttatca tatcaatgta
     2221 agtcccggca ggaggtccat cggtgagaga ctcaagatgg agatcctcca ccacctttgt
     2281 gtccgtctca tcggcaatct gccgggcaag tgcgaggttt tccacttcat ccaagaatat
     2341 cacaggagtg ccactggcct tgatctggtc tatcagtctc gccatctgct cagctgaagg
     2401 cgcggcatcc gagctcgcac tgggcaacac agcgccgatt acacgaaaac catagcgctc
     2461 cgcaaaataa cccagagatt catgattcgt caccagtaat ctgcgctcag gcggaatttg
     2521 cgacacctgc tcactgatcc agctgtctaa ttcggtaagc tgtgcgatat aagcctcagc
     2581 attggattga aaaactgccg cgccctcagg gtcataatgt gtgagccctt cccggatatt
     2641 ttctacatac gtaatcacat tgttgggatc aagccacaaa tgcggatccg ttccgccctg
     2701 ctcactctcc acatctgccc ttggatgtag accggctgag gcttcgatga cctctctctc
     2761 acctcccgcg ttttcaagca gagattccaa aaaatactca taacctgcgc cgttgataaa
     2821 tagcaatttg ctgtccgcga ttttcgttac gtcttgcggt gtagcctgat aggaatgagg
     2881 gtcggcgcca acgggcagca gcgattctac atcgacgcga tcgccagcga tgttttgtgc
     2941 gatatctgca aggaaggtgg tggacgtcaa caccacgggc ttagtcgact ctgagggtga
     3001 ggtggatcgc cctccacagg cagagattag tgcaatgaca agtagaaatg aaaacaactg
     3061 tttcatttac attccgggca taaaccaaac agttgaagcc agtgttcgtt gatctgatac
     3121 cccgttcgac gcgcaatcga cttaataagg ctttccaaat catctccctc aaatgtaagc
     3181 gtctgaccac attctttgca cagcaataag tgttgatgac cggcgggtgc ggtgatgaag
     3241 gactggcaac cacgcggctg atgaacgcgc tggatcaatc cctgctcctc aagcgcttcc
     3301 aaagtgcgat acacagtaac caatcccagg gcagggtagg cctcccgcgc agcatcatag
     3361 acttcgatgg gggtgagggc gtggatgctc tgtgccatcg tttccaccac tgcacgacgc
     3421 gcgcccgtca aacgatagcc gcgttcattg agttgggtta gccagggatg ctcgttcatt
     3481 taagtttcct gataatgaaa atcgttttca ttttcaatta tagtaaaact cccccgcctg
     3541 tcaacccctg aaacaaaagt tagctttccc agcgaagaga atgatagatc actgaagtga
     3601 tgaccagtaa actaatgaca gtgcctgtga tcgtcgaaaa cgcttttcgt tccttgatag
     3661 gccaattcgg tcgatctgca ttcaatttcg cacctactaa ggacgctgca gaattgctgc
     3721 acagatcata ctgatcacta caccttattc ttgatccaaa tcaacgcttt cgtgaggcag
     3781 aacttccttc ttgaggagaa aataggcacc agtaaagtgg tcatccacgc cgcccaccag
     3841 catcgtgcgt cggcgcggaa tgatgttgat cacttcccag ccctcgagac cgtgattttc
     3901 tagcgctgga acatcaaagc cttcattgag tggatcatca cttaagatcg cgccggccgc
     3961 gaggtagacc attttataac gaaatacctt accgcctttt tgcaaacgca gccttgcggt
     4021 ctccacgcgt gttcgacgct cgcgatcacg ttctgcctca atttcagcgc gcatccttgc
     4081 caattctgga gatgccaaaa tctcattggc tacttcgagt atcgaatcat catcactcat
     4141 cgttgcagtt attagaaatt catatccccg gcgatcaccc agttttacca gtccctcggc
     4201 tgcgtcgagc tggttgatcc attcgtcgtc ctccgtcatt atttcataga gtgactccac
     4261 gtcacgcatt gcaacgaggt ttttgatctg tgaattcata tcatcctccg gcaaaaaatt
     4321 cttagaaagc attgtatcac tgcaaattaa tgtcccactc catacatcgc ctatacaacg
     4381 taacctatgt taattatggg gggttttaat tcgggatata gtagagtatt attcagacta
     4441 caaaaaggaa tgtttagagg attcttcagt cagattctgg ttgacctggg agatatggaa
     4501 ggggactatc gtgtctttca tcttaagaat gatattgcac aaagcattct ttctttaagc
     4561 attgccagtc ttagcatttt gggcatgttg agaatggatt cctggtacct gaggaacgac
     4621 cccaacctgc tgatggtgct gatgctgtat cgtggcgggt ttgtcatcgc caccgttctt
     4681 gtggtgttgg cgatcaccag aatacgaaag gtgaggattt acgaccgtct cctgcttggg
     4741 tgggtatttt gtgcgatctt gttttttgtg ctgtcgaatt tcacacgtcc tacaaattat
     4801 ctgtcgacgg cgtcagacat tattgttcca tttgcaatct atgtgctttc gcctttcaga
     4861 gtgttgtata gcatcgctct ggcttttggt ttttcgatgg gcaccttgta tgtagatcat
     4921 tttttcaaga ccggggtaga cccgatcatc ctgagtgcgg ctacgtctgc tcagattgtt
     4981 gtccacgcgc tggggcttgg gtccgcatta cagattcaat cctatcgtcg gcggtcgttc
     5041 aaagcataca taaaggaaaa agacgccaaa gaaatggtct catatttggc aaacatcgat
     5101 ccgctgacaa aaagtatgac ccgccgtcag ttttttaaca ttgccgaaac tgaattcctg
     5161 cgcttttcac gatatcgccg accattttcc gttctggccc tcgacctgga tcgttttaaa
     5221 gatgtaaatg acacccacgg gcatcatgct ggcgatctgg ttctgagaag tttttccttg
     5281 gtggcactgg agcaaaaacg ggcgcaggat acatttggca ggttaggtgg agaggaattt
     5341 ggactactgc tccccgaaac caatttggaa caggcaaaag tagtagcgga ccgcattcaa
     5401 aaaacatggg aacaatcgcc agtcaacctg gacggtgtgc caatttactc caccgtgagc
     5461 attggcgtag cagaagcaaa ttcagaggat aaatctctgg atgatctcct gcggcgggca
     5521 gatcaaatgc tgtataaagc caaggaacgt ggaaaaaatc aggttgctgt agaatgaaca
     5581 actccctctt ttcgagggag ttgtttttac acaatcattt catttatctg ccactactct
     5641 ggaggatcag gtaaattacg aatgcagtga cgagcaaaat tgaaatttgc accgaaggac
     5701 gcgtccatac ggacctgcga cgatattcat tgggggtgcg tcgacgacga cgctctcgat
     5761 atcttctata caaggctgcc tctttattga aatgatttca gcgcgctatc aagatcttta
     5821 tagaccttga agaacagctt tagcccggtg gtttcaagcg ttctgtccac atctggctgc
     5881 gggttgagga gtttcactct tttgcgcgcg ttggcatgat gggtgaaatc agggcgggtt
     5941 tccttgcctt cctgctctac ctcctgcttg ccgccactat agatcaaggc gatcttatgg
     6001 atcgctacaa gtcccgcact gctgatatac ggcacttcgc tcaaatccaa aatcaaattc
     6061 cgcaccggat tattgtagat ctcctgtgct ttgttgacga cttccacata atttgcagca
     6121 tcaacattac ctttgatgtt catcaccgcg accggctcat tcgcctcatg aatagacact
     6181 tgtatttcca tgattcctca actttatgca ctaacggaag ttaaattctt cgtaatgaag
     6241 gttttcccgg ggtacaccca attccaaaaa ttttttctcg aacgccatgg tcattggcag
     6301 aggaccacac atatatacgt gatacgatcg tatatcgccc tgtgcgtttt ttacgatatc
     6361 ttctatcctc aatgaaccat ctttagccga atatcgaata tgaactctta aacgggagtt
     6421 tttcgtatcg gccgcctcaa gttcatccac aaaaattgct tcctctggat ggcggacggt
     6481 gtagtaaaga tccacatcct gcgccagcga gttgtccatg tcgcgcacaa acgagagaaa
     6541 cggcgttagg ccgatcccgc ctgcaatcca tatttgtttc ggtccacccg tcttgtaatt
     6601 gaacatccca tacggtccct cgattgttgc gtcagtgcct tctttcaaat ggctgaacag
     6661 gtctctcgtg aagttcccgc tggctttgat cgtcaggcgc aaaacatctt ctgaaggagc
     6721 gctcgaaatg gtaaacggat gagattcgtt caaaacctta tccttcggaa atcgcacgaa
     6781 caggaattgc ccggcacgat gcttcttcat cgggtccttc ttcgggcgca aagtgacttc
     6841 ggtgcttgag ttgttcggat gcttcaccgc atccacctgg tagggccggt attgtttgaa
     6901 gataccacca aagaattcgg tataaaggta ggaaaccgtt ccaataataa agaaaatcat
     6961 cacccaagtg attgcaattt tcgcatccag ggctttcacg gtcagcgagt gaaagaatcc
     7021 caaaatgaaa aagatgccaa tatagcgctt tagtttcttc caatcctcat aatcacctcc
     7081 actaagtttg ttgagaaaag ggatacgcgg cgccagcgta atcaaaacaa tagtaacgat
     7141 cccggtaaag gcgattacac ccaggtaatt tcccagcagc caacccgtgg tcgtaatggg
     7201 cacggtgagc acatgagcaa aaatcaacag aaaagcggct gtcccggtgt gacggtgggt
     7261 catgtacatt ttgtccagtc cgccaaaata tggttcggcc cactttggac gagtagaaag
     7321 aaaaagagag attgccatca gaacaatatt attcgagcca atgacctcgc caacatacct
     7381 gcggataaag ttgggctccc catcattaac cggcggaaaa acaacccata caacaacgtt
     7441 taatatcacc aggccgatga tcgcccagtt gccaaggtta ctgcgcttca tagtgtctcc
     7501 gggatgcgat gcttgcagtg atcaaacagg taccgcttat cgtggtaata atccaaaatg
     7561 acagctttat gagtgcctta cactccataa ttcaaaggcg gtatggtaag aaaccatgct
     7621 gctgcccgga tgactcaaaa tgacttcacc gcttccgttc gatccgtgaa aatctcaaac
     7681 accttgtcga agcccaccat ttctaacacg ctcctgactt cctcgctcgg attcagcagt
     7741 ttgatatgtg cttgcacacc cgcctcactg ccccgtccca tggaacgata agcagaccaa
     7801 ccatgctctg tgtcgggcat ttcctctccc tttagcataa gcgcaatgct gtgtaacgcc
     7861 accaaaccag cgctgctaat ataggtgagg tcggttagat cgatcagaaa gtcatgtgca
     7921 ccggcatcaa acaattctct ggcttttgta atcaagctct ggtaactctg accatcaagt
     7981 tgaccttcca cgcgtagagc ggtcacgtcc acgcggcccg cttcttttga gactgaaata
     8041 ttcatttctc ctccttacaa cgctgaattg aattgctcaa ttatacttcc cctttcacat
     8101 ttatctctta ttctgcggac tctcatgaga aaccctgaga aaatttagac agatttcttt
     8161 ttatcgtcgt gttttctttg caggcgttcc aaacgatgag taatttcatc ctgccagtgt
     8221 tttctcatat actcaggcat atctgttcgt ttcgcacact tcattgccaa tttcgtccat
     8281 ttgatggcag ttttgacgtc gcgggattta tgttcatgat gcttcgccag ttcaagaaat
     8341 gcatacagat gaccacgctc tgccgcatct tcccacaatc ggagcgcttc attcatgtcg
     8401 ccgcgtttct tttgcaggat agccaaccgc ttcactgcca cgccgaagtc tgatgccgtt
     8461 aaatcggact ctgcctccgc cccagactca agcccgcgtt cgaacaggcg ggctgcttca
     8521 tcccaataac ccagatcttc aaacagtttt cccagcgcga tgaaatcgag accatgctgc
     8581 acatgcccat cgtacggagt ctcgagcata ttgttcatgt gtgaaagcag agcagccatg
     8641 gcaaccacat ccatggcgtt gtgatagaag actcccgcta acggacgtgc atcccctgta
     8701 cgaagataat caaaatacaa ccacgggatt tcataaccgg gtacttcttc ggtagaacgt
     8761 gccaggccaa ggatgttttc ttcgagatat ttcaacgcgc gcgagggtag acgatctcgc
     8821 cacaatctgc gcgccagagg taaaagatcc aggtgagagt agtccctgta aggcacagga
     8881 atacgatgta aggagtaacg agtggtgagc aggggtgcat caaaggcttt gccattaaaa
     8941 gtgactaatg ccttgcaggg agcaagaaaa ttcgcaagcc cttcaagcaa agcaggttcc
     9001 tctgcagggt ctcgcatgaa gaattgccgc aaaaggaact ggtcatctac aaagcgcgca
     9061 accccgacca gaaacgcata tgtccctgtc cctccggaca gtccgcttgt ctctgtatcg
     9121 aggaaggcaa actcagagag agggagttca gcaatccgtg cgtctctggc ccattgagaa
     9181 atgaccgaga tggggagaga acaggtaagt gaagacaaac cgtgacgata gtcgatcgtg
     9241 tagtcttgct cggctacaaa tacgtcaccg agggcggtag ggtgaaacgc accagccacg
     9301 accgaatcga tcccataatg gggtttaggc tcgggcggtg cgagatccgc agtccccact
     9361 ttcacaccta aggcttttag cctatccgcc agagaggaat tgcgggcagg gttaccagaa
     9421 ctcatgttcg atgattataa ccaaaggaga cctatcgaac aaataaaaat gtccaccaca
     9481 ccgtgatgga cattcacaaa atatgcagcc tgagttgtga ttagtaaacc ggaccccacc
     9541 ataactcatc tgcagaaaaa tcggtttttg gaacagccac cacaaacaaa cgcccccaac
     9601 tcgaaattcc ttctgcgtaa atacaggtct gaaacgtaac atggtgctca cctgcataaa
     9661 cacgattgaa catttgctca gcagttaaaa ctgtatcgtc ttttaggtcg cgaaatgaac
     9721 tccaggggct gttgggttgc aacgcttgat atttcaatat ttctttcaca acgaaatatt
     9781 ccacttttcc gtccccatat actaaacgga cttcctgacc gactgccaag tgcgaaaact
     9841 ctttgccaga taaataattg tgtgccaata agcctacatt gccgtaccga gaggccatat
     9901 caaattgtgt tacctgtcca tcattggttg agacataacc aaaattcccg gccggttgtt
     9961 gcacaacggg caaggcaagc acattcggaa catagacacc gcccagaaga ttcgatgtgc
    10021 catttcgaac cgcctgtgaa aactcagtga aattcggcaa ggaaacatta ttattttcca
    10081 tcgccgaaac tgcaccatga ttaagaaacg tggaaataaa aatgaagaac agtaatcccc
    10141 tgctaagaac tattcgttgt atttgacttt tgaacgccat tttgattctc catttcaata
    10201 aaggcgctca aacgaaggca gagtgaatga actcgatcgc cttcggtaac ctggccgtca
    10261 tggttgcgct ggtcgttgga gcgcaactgt agatccatgg ctttgcgtcc ccgcttttca
    10321 gcgggtttgc ctttatcgaa cgaacagatg ataaggtact agaaaaagag cctggcaaat
    10381 gaccaggctc gcgaagctat cgacataaat ttcatcttcg gcgacctggc agtcgtggtt
    10441 acgctactac gcaacggtag acccatggtt ttgcgtctcc gtttttcaac gggtttgctc
    10501 ttatcggatt tgattggtca cattatagca agctacggta acttcatcct tttcatttaa
    10561 gtaatatgca cttacaaatt tgcaacgaaa aaattactca gatggctatt gaaggagttg
    10621 ctgaaagata gtttcaaaac ttaaagcctt tggcaccgcg ataacaaaca gcctgcccca
    10681 actggcattc ccgtttgatt cgatgcaggt ttggaaagtg acgtggcgat ctccggcgta
    10741 ggcccgcgta aacatttgtt cggtggagag aatatcttcg ctttcaagat ttcgaaacga
    10801 actccacggg ctgttaggtt ccaaggcctg ataacgcaaa acttcagtga tgacgaaata
    10861 ttcgactctc ccatccccat aaatcagacg tacttcctct cccaccgcaa gctgcgaaaa
    10921 taacctgcct gataaataat tatgtgccag cagacctata ttgccatacc gagacgccat
    10981 tgagaactgt gtcgcttcat cgtttcgacc agagacatac ccgtcgtcgc ctgtcggctg
    11041 ttgtacaaca ggcaaagcta acacacttgg aatatataca ccagaaagaa catctacatt
    11101 gccattctga acctgttgga tgaagtccaa aaaatcggcc ggtgtaggtt cgccgtttcc
    11161 cgtaccaaca ttgaagatgg gagtgaaaaa cacgaggacc agacctgtca acatgccccc
    11221 tccaaagatc attcgggcaa atctgttttt gagtgccatt ttatgtattc ctttgcttcg
    11281 atgaaggcac ccaaactaac tggcgaatat atgattcgct ttcggcagcc tggcggtcat
    11341 ggttgcactc atgtgcaact gtagatccat ggctttgcgt acgcgtcttt cggcgggttt
    11401 gccttttcga taaaacgtgc gcattataaa agaagtaaag tctttactgg tctgtgaata
    11461 ccctgcggat tccctacgat ctgaacagct ttatttatag aggttctttg caaaacgttc
    11521 ctatatgtat gctaaacttg ctccttcaca agagcatgac tacagaatcg agagaacgcc
    11581 ttccagtttg ggtgaaattg ctttacggct cgggggattg gggtatcgcc agcattggca
    11641 tcatgcgttc cattttttat gccatctatc tcacggatgt agtcgggttg gaaccgcgca
    11701 ttgcctcttt cggcgcgatc gtgggcatta tttgggatgc gatcaacgac cctatcattg
    11761 gtacctttag cgacaggttg aatacacgat ggggtcgccg gcgtcctttt ttgttatggt
    11821 ttgctattcc atttgcgttg agttttgtca tcttatggtc tgcgccacaa tgggaaaatc
    11881 aaattgctct tcttatttat gtgacactat cctttatgct ggcggataca cttcagactc
    11941 tgatctcagt cccatttctt tctctaaccc ccgagctgac tcccgattat gatgagcgca
    12001 cctcgctgac aagtttccgg tccttctttc agttggtagg agcgttgacc gttgtcattg
    12061 ccgcaccgat tatcgttgac cttatcattg aatcaggtgg ctctcaacaa cagggattta
    12121 tgctggtggg tgcgatcttt ggctcgatca gcgcggtccc tttactctta atcggactct
    12181 tcctccgcga atcgtccaaa ccggacgaaa ttaaatcgat cccttttaag gagactttgc
    12241 gcctcgcctg ggggaatgtt ccgttccgct acgcggtcgg cattcacatg ttgaactggt
    12301 ctgcggtgga tatgattgca gtggcatttc catatttctt actatactgg gtagcacagg
    12361 gagatttgct tgccaagata cgaatctttg gcatcgatct tgcctatgaa tctgcctttt
    12421 tcggcatcct catgagtgtt tgcatcctgt ttattccctt ctggttatgg cttgccaaaa
    12481 aacgtaacaa gcgcgaggca tatattcttg gcatggcatt ttggatcatt gtggaattca
    12541 tggtctttac gattcaaccc gggcagacga attatttgat cgccatcgct gccctggctg
    12601 ggatcggcgt atctgctgca tacacactgc cagatgcgat gtttgccgat gtaattgaat
    12661 gggatgaact ccgcacccgc cgccgccagg aagggattta ttacggtgtg cgcgctttta
    12721 ttcgcaaagt aacgggcgca ttaatggtct ttattacctt gcaggcatta ggctggtctg
    12781 ggtatgtgtc gccgcccgaa agtgcaaccc agttcaccca aggcgacaca gctttgcaaa
    12841 tgattcgcct gttggtttca ccattcggcg cgctgatgct gtgtggcacc attatctttg
    12901 cctggctgtt tccgttatct cgtgaaacgc acggacgcat acaaaaatta ctcgaaaaga
    12961 gaagagcgcg aattattaaa gtagaaaccc cgagtccttg aacccggggt ttttacttat
    13021 cgttgaccca ttgtaaaata caaaatcggt agtacgccgc atagggcaca atcaaacaca
    13081 attaacagaa gccacatcaa ccactgcgcc ggtgctttcc tttccaatac cgcgtccgct
    13141 ggattttgtg gattatagaa aaccatcacc tgcgcacctg cggggtaacg cgctacaact
    13201 tttcccgcgc ccgtcccgcc gacgtctggc cccggtgcca gttttgagct ttgataactc
    13261 tgtccaccga tttgatagga atattgcaca accgggtaat tcgtcgagcc gccctcactc
    13321 gaagacctgc gctcaagata ggatgaattc actgtaccca tcgtggaggg ccactggctt
    13381 accgccgcca ttttcctgcg cagaaaaaag ataataccca gaaaaattgc attgaggata
    13441 attaagacaa aaacaataat accaaccacc agcaaaaatt cagtgttcat gtttgctcct
    13501 ttattgtttg gtagtggtga cttcagtcgc ttttttcatg atccaatatt tcaacgactg
    13561 aagtcgttac tacggcatta caaccgtcac ctgaatcact gcactatcca cacgctgatc
    13621 acttcgtacg acttgcaact gcacagcata caacccactc aaaccggttg tattccattc
    13681 tgctagcagc ccatcggtca caggtccagc attttccgcc acttgaatcc actcttgcgg
    13741 attcaatccc tgtcccacta aaagccgata cgagacaaaa ttttctccag cggctgtacc
    13801 tgaaattcgc acttcccctg taacttcagc aaacagctca ggggacgtta tgttgacatc
    13861 cggatttatt tggggtgact gtataacatc ataggtcgtg ggaggaatct caacgccggc
    13921 gctttcagcc caggaacgag cgtctgccgg aacaacaaaa taaacgcggt cttcaacgag
    13981 ttgcggcagg gtaaaaacag ttgcgagaaa accggtctca cggttgatcg caaatctgcg
    14041 atacatcgca tctggttgga ctggttcatt tccgttgaga aatacttcac tcaccaaatt
    14101 cggacaatct gttgttggca acatcccaga ggggtcacag acattaatca cagagacatc
    14161 ggctgggaca gcccagccat cacctggtaa attctgtgaa gcagtttgca ttagcgcgtt
    14221 ccacaatacg gcgggcacgc ggggcgataa cgctccttcc tgccgcgcgc cggtccacac
    14281 cacagctact ctggacggcg aataaccaat tgcccatgca tcaagccgat catcggtttg
    14341 acccaacttg acagctgcaa ttctcccaa
//
LOCUS       DASTJP010000216        12872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_779757, whole genome shotgun sequence.
ACCESSION   DASTJP010000216 DASTJP010000000
VERSION     DASTJP010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12872
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779757"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFQ23_06625"
     CDS             complement(<1..775)
                     /locus_tag="VFQ23_06625"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9906294.1"
                     /translation="MTLPNFMVIGVAKAGTTSFYHYLDQHPQVFMSPEKGSNYWGYED
                     ARNWKWSEEEEGKPPLLRHFMARTFEDYQALYDGVTNEIAIGESSPQYFRCPTAAQRI
                     HDAIPNVKIVASLRNPADRAFSGFLMRTRRGEPVKGFYEELTADASHVRESLYYKRLK
                     RYFDLFPRDQIKIIIFEEFRKNPAQTLVDLFSFLGVDPNFVPDTSVQHNPAGVPKIRL
                     LNKLFFHPTLIRTAKAIFPESVHERLKQVQQQNLKAPPKF"
     gene            complement(807..1958)
                     /locus_tag="VFQ23_06630"
     CDS             complement(807..1958)
                     /locus_tag="VFQ23_06630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9906295.1"
                     /translation="MSESKEKLKILRIILAIRETSAPYNQFSLAWADKHDITICTYFA
                     SDTVPSKTVDVFEGNGSVRGFFQALKAALHGKEYDVIHVHSPHLGCLFLLAAIFFYRT
                     LLNSTIMTVHDSYQNYKIRNKLMFIPVFASFRRIVCCGQASYDSFPAFYKWLAGDRLC
                     AIPNSLDVARVDRIAANITQRRQSNLFNVVAVSRLLDIKNPFAVLMAFHQGSDETGRL
                     TYMGDGPFRPALISKSKELGIQDRAEFTGVVPREQVFESLLNADLFISTSRGEGLPVS
                     VLEAMACGCPVLLSDIPPHREIAESLSFIPLIQQDDIAGFAREIKKFTAMSVSERKSL
                     GQACRKVIEEHFSLPVMHAAYEEVYRQVMNKRSSTTFKTNVLSGNKPEA"
     gene            complement(1994..3127)
                     /locus_tag="VFQ23_06635"
     CDS             complement(1994..3127)
                     /locus_tag="VFQ23_06635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9906296.1"
                     /translation="MNKSSEMAIHQKPKLAIFLPSLSGGGAERSMLNLAHGVAERGYP
                     VDLVLAQARGPYLSLVHKDVRIVDLKASRVLASLPALAGYLKKERPKALISALNYANV
                     VAVWARSLAGVPTKVLVNEQNTVSISAFNSAKRRQRMVPYFMKYFYPWADYIIGNSQG
                     VAEDLSEVTGLPPQRIDILYNPVVTPEVQEKVHAPLNHPWFQPGQPPVILAVGRLTKQ
                     KDFPTLIQAFGQVLKNRSARLMILGEGVDRPKLEALVKELRLEDHVSLPGFVENPYAY
                     MKRASLYVLSSRWEGLPTVLIEALYCGRPIVATDCPSGPREILAGGQYGVLVPVGDVA
                     ALSRAIVAGLAGETPLPTKESWHPYSLEAVSDQYIDLLLNGHS"
     gene            complement(3161..4402)
                     /locus_tag="VFQ23_06640"
     CDS             complement(3161..4402)
                     /locus_tag="VFQ23_06640"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9906297.1"
                     /translation="MRTVTFLLSLILNFMIPWEGVVRIGGVGTGVKIFGYILAAFWLA
                     TIVITRRVRKPGTFQVLLCLLVLWYALSVFWSENPSRSAAHAVTWFQMLLLAYIWWDL
                     YTTRTAVLAGLQSYVLGAYVSLGSAIGNFMSGKVFYSHYDRYSAGETNPDGFGFLLAV
                     GIPIAWYLAISENTAGKARWLRFVNYAYIPLAFMGIALSGTRTALIASVVGTAFGLAS
                     LTRVRIWVRIVILLLLIAASFYLLPYVQNLRSFERLGTTGTELTQGDLNNRTNNWQEG
                     FAAFREQPILGIGGNMYPTVNRWGKAAHNSFMAVLVELGLVGFLIFGGILLIVFTEAW
                     RQPKWESRFWLTVFGVWILGSYTLTWTHRKPTWLLLSLLIAAASLTVRREEAVYHEEA
                     VPLPHHRDPEILAVPHLRPSK"
     gene            complement(4467..5222)
                     /locus_tag="VFQ23_06645"
     CDS             complement(4467..5222)
                     /locus_tag="VFQ23_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906298.1"
                     /translation="MKIGTNNKFVVLTTNRSGSEWVMSTLNRFPNVFAHSELFLPRRQ
                     VSEGKWNSDFAYPRFVESKVQGVALRPFSVFSYLNTFYGMPGKVGFKLMYKQLGLYPE
                     ILGYLLKHHVRVVHLMRQNHLDVMLSYAVKAKLGRSHLLAGQSAPEDLRVQLDTRTLV
                     RNLDWLQEQQNMARRMLKIFKLPHVEVVYEELVRNHKLFLQLADFLDIDSEQQIPEFS
                     LTKIRKEGHRDVIGNYDEVKEVLLHSKFAGLLE"
     gene            complement(5235..6728)
                     /locus_tag="VFQ23_06650"
     CDS             complement(5235..6728)
                     /locus_tag="VFQ23_06650"
                     /inference="COORDINATES: protein motif:HMM:NF007773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="HET9906299.1"
                     /translation="MGLQQKAVKGIFWSFVQKWGRAGVTTVTFVILSRLLAPEAFGLV
                     ALATAFTVFIELFLDQGFGAAIVQRKDLDPEHLDTAFWLNIGSSVVLTGVLIAASDWL
                     AVLFDEPQLGAVLRWLSITLLLSGLSGTQMSILQRNLSFKDLAIRSTVSNLVGGVVGV
                     GMAFAGYGVWSLVGQDLATSVVRVIILWQACDWRPGLKVSKKHYKELVSFGIPIVGSH
                     ALTVLMRRSDDLLIGYFLGPTLLGFYTIGYQLLLIIIKLVTEVTNSVAFPAFSRIQAQ
                     PERMRQAFFKVTQYTSVIAFPIFFGLAILAPELVLTVFGEKWTPSIPVMQVLSLIGIL
                     QSVLFFNGSVIKAAGKPSWQLGIMVLNAVCSIVGFLLAVHWGIVAVAASFVIVGYLLA
                     PISYIAVRRLIQIDIRTYLSQFTPSFTASLLMVAVIAGLKFLLKEQPFNLYLELAVYV
                     LAGALTYIAVIGLLARHLYPQALEIVNLALPKWKSRKVPDSKPEIPG"
     gene            complement(6756..7592)
                     /locus_tag="VFQ23_06655"
     CDS             complement(6756..7592)
                     /locus_tag="VFQ23_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906300.1"
                     /translation="MLVFIHINKTAGRTVRYILRSSFGVYHCEAEPWHARDSSDQPFS
                     AHDLKLSRKVYPRLESIGGHRVTGYTDLGESGTEFKYFAFMRDPIKTCASRFQYNIQY
                     RGKQNLDFEEWIQRDWTRNSQTKLIAGSADVDTAIRIIKEKNIFIGLTERFDESMLLL
                     KGLRAKNLNISYQRINVATNNSIAQKILSDEKTRQLLIEANRVDQQLYDYVSQELFPL
                     WQKEYGPSLANDLVNYQQTREGSFNQWNLTTSRLKQFMVYRPLIYLYRLSTGRKSRLA
                     LP"
     gene            complement(7615..8340)
                     /locus_tag="VFQ23_06660"
     CDS             complement(7615..8340)
                     /locus_tag="VFQ23_06660"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9906301.1"
                     /translation="MINEVAVADKLKAGSPLIMELVGPAGAGKTTLSKALRQRDDNIL
                     LGPDIELRKVSHLPVFIRSIPSSLPLFFDQSKSDRQPTWTELKDIAYLEGWTKVLKRI
                     PSSNSRTVLLDQGPVFRMATLYEFGPESFRSEKAKAWWQHLYEQWAAALDVIIFLDTS
                     DEILMERINVRTKKHGIKGKSELEARNYLMQYRMSFRDVLTNLSMRNGPTILEFDTSK
                     TPIEKIVDEVLVACESKRTENSF"
     gene            complement(8337..9908)
                     /locus_tag="VFQ23_06665"
     CDS             complement(8337..9908)
                     /locus_tag="VFQ23_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906302.1"
                     /translation="MTISLETSSTLDQVRPEGAVQPTNYAVLPIVKKLCDALRVENID
                     YCHWKSNNMLARSANGDNDLDLLINRADETRFAEILFRLGFKQVTAPGEKQMPGVLDF
                     FGFDHDAEKWVHVHAHYQLIMGHDMTKNFRLAVERPYLESAVQGELFRVPAVEFELII
                     LVIRMVLKHSTWDAILGREGKLSSSERKELVYLQERSDSQYVNQILRKYLPYIDVDFF
                     KECVDALKSGASLWKRVKTGQELQIRLRSNAVYSIPVDTFLKLWRRAALIIQRRLFKP
                     STKYQLAIGGAVIALLGGDGAGKTTAVGAISTWLSKNFKITKIHMGKPAWSPITVTIR
                     SILKIGQVLGLYPLETTFDETIQQKSRVSPGYPYLIREVCRARDRYWTYVKARRLAAQ
                     GSLVILDRFPLPQIQIMDGAQAEQFVRQLQAGPRANQPLSPRLNHWFARYLIRLEESY
                     YKQILLPELLMVLRLDPEVAVQRKTDEDPQSVRTRSTEIWKLSWENTDVHVIDASKSK
                     AEVASEIKTLIWSQL"
     gene            complement(9921..11201)
                     /locus_tag="VFQ23_06670"
     CDS             complement(9921..11201)
                     /locus_tag="VFQ23_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906303.1"
                     /translation="MGHDLTKQYSGFFAKNGVKKIFSLAKDIYKFGKFRELVSFAVWE
                     AYHKFGLFPKKDYLSFSKADAIALENEKLATLEFDEKVSRFFGSKGLRATRCEHNWKQ
                     IYINNNGEVFGSLYPDDCDLYKSVDGGKTVEFIYKFPESIKSLFISSQNTIFVCLEGS
                     MYRGFEKNKSFKKVLDLGSPISFFRSNNAMTETPSGELWAGEYGNILEKNGWRKLAYL
                     YHSADDGETWHVSDFLIQKGINKHVHIVKYSKVLNRLMVADGDNYKKLWISGPLDTFD
                     FKNPNWIPITKYHIQMGGYTAVVENDEKVFFGTDYQGGTNFIVETTDAKTFTKKIVPD
                     PYRRSPIDNMVMRKSKQGYEMWANLPFSAPGTRCLLMYSNDGGSSWNKVFDYSRSTHA
                     AWIISSSKETTDTVYVSIQNQKTNARVVYQLVDA"
     gene            complement(11603..>12872)
                     /locus_tag="VFQ23_06675"
     CDS             complement(11603..>12872)
                     /locus_tag="VFQ23_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906304.1"
                     /translation="TASSENIDALEVLQDGRLLISTTGNPTVTGVSGAADEDILQFTP
                     TTLGANTTGLWTLYFDGSDVGLGDVSSEDVDALDIDASGNIYLSATGLFSVPGVSGDD
                     EDVFVCAPSSLGSVTACSYFSALFFDGSAWGLAANDLDSFNLSFTGPVPTATPSNTPT
                     KTSTPTTTATTGPSPTFTNTPTATATRTPTNTPTTGPSPTFTFTPTATNTPGGSDVIF
                     ADGFESGNLSAWTSNSNDAGDLSVSPSAALVGNYGLQAVVDDTNTIYVTDDTPNAEPR
                     YRARFYFDPNSITMANGDAHFIFKGFLGSGTDVLQVELRNSSGAYQIRGKILNDASSF
                     VVTNWFTISDAPHYIELDWRAATGAGANNGGLTLWIDNVQQANITNVDNDTSRIDRVR
                     LGALAGMDAGTSGTYFFDAFESRRQNYIGP"
BASE COUNT         3426 a         3155 c         2959 g         3332 t
ORIGIN      
        1 ggaattttgg aggtgccttc aagttctgtt gttgaacttg tttcagtctt tcgtgcacac
       61 tctctggaaa tatcgccttg gctgtgcgaa tcaacgttgg gtgaaaaaac aatttattta
      121 gcaatcggat ctttggcaca ccggcaggat tatgctgtac agaggtatct ggcacgaagt
      181 ttgggtcaac gcccagaaaa ctaaacaaat ccacaagagt ttgtgcggga ttctttctga
      241 actcttcgaa gataataatt ttgatctgat cgcgcgggaa aagatcaaaa tatctcttca
      301 gccgcttata gtacaagctt tcgcggacgt gactggcatc cgcagttaac tcctcataaa
      361 atcccttgac gggctcgccg cgacgcgtgc gcatcaagaa tccggaaaag gcacggtcgg
      421 cgggattccg caggctggcc acgattttca cgttcgggat ggcatcgtga attcgctgtg
      481 cagctgtggg acaacgaaaa tactgcggcg aagactcgcc aatcgcgatt tcattcgtga
      541 ctccatcata tagcgcctgg tagtcttcga acgtccttgc catgaaatga cgcagcaagg
      601 gaggttttcc ttcttcttcc tctgaccatt tccagttgcg cgcgtcttcg tagccccaat
      661 agttggaacc cttttcggga ctcataaaaa cctgagggtg ttggtcaaga tagtgataaa
      721 aggaggttgt acctgccttt gccacgccaa taaccataaa attgggaagt gtcataatgc
      781 ttctcctaaa tagtagtgaa cttaaatcaa gcttcgggtt tgtttccgct aagcacgttc
      841 gtcttgaagg ttgtcgaaga tcttttattc atcacctggc gatatacttc ctcatatgca
      901 gcgtgcatta caggcaagct aaagtgctct tcaatcacct ttcgacatgc ttggccgagg
      961 gattttctct ctgagactga cattgcggtg aactttttta tttctcgagc aaagcctgca
     1021 atatcatctt gctgaatcaa cggaataaaa ctcaagcttt ctgctatctc gcgatgcgga
     1081 ggtatatcag aaagcagaac gggacatcca caggccatcg cttcaagaac agagacgggg
     1141 agaccttctc cgcgtgaagt ggagatgaat aaatctgcat tgagaagaga ttcaaatacc
     1201 tgctcgcgcg gcaccactcc ggtaaattcc gcccggtcct gtatacccaa ctccttactt
     1261 ttgctgatca gcgctgggcg aaatggcccg tcgcccatat aggttagcct gccagtctca
     1321 tcactgccct gatgaaaagc catcaagaca gcaaatgggt tcttgatatc cagtaagcgg
     1381 ctaacagcaa cgacgttgaa gagatttgac tgcctgcgtt gagttatatt cgccgcgatg
     1441 cgatccacgc gcgccacatc caaactgttt ggaatcgcgc aaagcctgtc cccagccagc
     1501 catttataaa aagcaggaaa actatcgtaa cttgcctgac cgcaacacac aattcgtcga
     1561 aagctggcaa acactggaat aaacatcagc ttgttacgaa ttttgtagtt ttggtagcta
     1621 tcatgaaccg tcattatggt cgagttcaga agcgtgcggt aaaagaagat tgcagctaag
     1681 agaaacaagc agccaaggtg cggtgaatga acatggatga catcatattc ttttccatgt
     1741 aaagcagcct tcaaggcctg aaaaaaacct cttaccgaac cgttgccctc aaacacgtct
     1801 acggttttgg aaggaacagt atcagatgca aaataagtac agattgtaat atcatgcttg
     1861 tcagcccagg cgagagaaaa ctggttgtaa ggagcactgg tctctcgaat tgccaggatg
     1921 atacgcagga ttttcagttt ttctttcgac tcagacacag tacagattct cctcttgttt
     1981 tgctaataaa ttcttagctg tgaccattca ataacagatc aatgtattga tctgagacgg
     2041 cttcaagcga ataggggtgc cagctttcct tagttggaag gggcgtttca cctgctaaac
     2101 ccgctacgat agctcggctc agcgccgcta catctccaac gggaacaagt acaccatatt
     2161 gcccgccagc caggatttcc ctcggtccac tcgggcaatc ggtcgcgacg atgggccggc
     2221 cgcaatacag tgcctcaatg agtacagttg gcaaaccttc ccagcgcgat gaaagcacat
     2281 aaaggcttgc tctcttcata taggcataag gattttcgac aaatccaggt agagaaacat
     2341 ggtcttccaa acgtagttcc tttaccaatg cttccagctt tgggcggtct accccttcac
     2401 caaggatcat aaggcgggca gatcgattct tgagtacctg tccaaacgcc tgtatgaggg
     2461 tcggaaaatc cttttgtttt gttaaccgcc caaccgccaa tataacgggc ggttgtcctg
     2521 gctgaaacca gggatgattg agcggagcat gcaccttttc ctgtacttca ggagtaacca
     2581 ctggattgta gaggatgtca atccgttgag gaggcagccc ggtaacctcg ctaagatctt
     2641 cagcaacacc ttgtgaattg ccaataatgt aatcagccca ggggtagaaa tacttcataa
     2701 agtagggcac cattctttgg cggcgcttag cagaattaaa agctgatatc gataccgtgt
     2761 tttgttcgtt aactagaacc tttgttggca cccctgccag actacgtgcc cagactgcca
     2821 caacattcgc ataattcagc gcagagatca atgcttttgg gcgctccttt tttagatagc
     2881 ctgccaaggc tggcaaactt gcgagaacgc gtgaagcttt cagatccacg atccggacgt
     2941 ccttgtggac aaggcttagg taaggtcccc tggcctgtgc caataccagg tccactggat
     3001 aaccgcgctc tgctacacca tgagccagat tcagcatcga acgttcggcc ccaccaccac
     3061 ttaaggatgg taagaagatc gctaattttg gtttttgatg aattgccatc tcacttgatt
     3121 tgttcaaggc cagacttctc cattacatct tatgttatgc tcatttactg ggtcggagat
     3181 gtggtacggc cagaatttcc ggatccctat ggtgaggaag tgggacagct tcctcatggt
     3241 agaccgcttc ctcgcgacga acagtaagag aggcagccgc aataagtaag ctcaggagca
     3301 gccaggtcgg ctttctatgt gtccacgtca gcgtgtacga acccagaatc caaactccaa
     3361 acactgtcag ccagaatcgt gactcccact taggctgacg ccacgcctct gtaaatacaa
     3421 tcaataatat gccgccaaaa atgagaaacc caaccagccc aagctcaacc aggacagcca
     3481 taaacgagtt gtgcgccgcc ttaccccacc gatttactgt aggatacatg ttgccaccga
     3541 tacctaaaat cggttgctct ctaaaagcgg caaagccttc ctgccaatta tttgtgcgat
     3601 tattaagatc gccctgcgtg agttcagtgc cggtcgtccc cagtcgctcg aaagatcgaa
     3661 ggttctgcac gtaaggcaac aaatagaagg atgcagctat tagcaacagc agaatcacaa
     3721 ttcgtaccca aatccgaaca cgcgtaaggg aggccagacc aaatgccgtt cccactacag
     3781 aggcaatcag cgcggtgcga gtgcccgaca gcgcaatgcc cataaaggcg agcggtatgt
     3841 aggcatagtt cacaaatctc agccagcggg cttttccagc ggtattttct gaaatcgcaa
     3901 ggtaccaggc gataggtata cctacagcca ggagaaatcc gaacccgtct gggttcgttt
     3961 cgcctgcact gtaacgatca tagtgactgt aaaaaacctt cccggacata aaattgccaa
     4021 tcgcactgcc cagggacacg taggcaccta gcacatatga ttgcagtccg gccaacacgg
     4081 ccgttcgtgt ggtgtaaaga tcccaccaaa tataggcaag caaaagcatt tggaaccatg
     4141 taactgcatg agctgcagac ctactgggat tttcactcca aaaaacactg agtgcatacc
     4201 agagcactaa caggcagagg aggacctgga atgtgcctgg ttttcgcact cgccgcgtga
     4261 tcactatggt agccaaccag aaagcagcca ggatgtagcc aaatattttt accccggtac
     4321 ccacaccacc aatgcggacc accccttccc aagggatcat aaaattaaga atgagagaaa
     4381 gtaaaaacgt gactgtccgc atggttagag tcctataaaa ataaaagaga tcgagccgta
     4441 caagtataca ctcggttgat atatattcat tccaataacc cggcaaactt cgaatgcagt
     4501 aaaacttcct tcacctcgtc atagttacct atcacgtcgc gatgcccttc ttttctgatt
     4561 ttggtgagag aaaattcagg gatttgttgc tctgaatcaa tatccaggaa gtctgcgagt
     4621 tgaaggaaaa gtttgtgatt acgaaccagt tcttcgtaga caacttccac gtgaggtagc
     4681 ttgaagattt ttagcattct tcgtgccatg ttttgttgtt cctggagcca gtccaaattc
     4741 ctcacaagag ttcgtgtgtc gagttgaacg cgcaaatctt ccggggctga ttgtcctgcc
     4801 aggaggtgcg accttccaag ttttgctttg acggcatagg ataacattac gtccaggtga
     4861 ttttggcgca tcaaatgaac gacgcgaacg tgatgcttca ggagatagcc cagtatttca
     4921 ggatacaagc ccagctgttt gtacatcagc ttaaagccaa ctttgccagg catgccataa
     4981 aacgtgttca aataagaaaa aacagagaat ggacggagag ccacgccctg gactttgctt
     5041 tcaacaaatc gagggtaggc aaaatctgaa ttccatttcc cttcagaaac ttgtcgtctg
     5101 ggaagaaata actcgctgtg tgcaaacaca tttggaaagc ggttcagggt gctcatcacc
     5161 cactcagaac cacttcgatt ggtggtcaac accacaaatt tattattcgt tccaattttc
     5221 atttcgcggc tccattaacc aggaatttcc ggttttgaat caggcacctt tcgcgacttc
     5281 cactttggca gcgccaaatt gacgatttcc agcgcctgcg ggtaaaggtg ccgagccaat
     5341 agccctatta ctgctatata cgtcaaagcg ccggcgagaa cataaactgc aagctccaga
     5401 tacaaattaa acggctgttc cttcaataga aactttagtc ccgctatcac agcaaccatg
     5461 agtagagaag cagtaaatga gggagtaaat tgggataaat aggttctaat atcaatttgg
     5521 ataagtctgc gcaccgcgat atacgatatg ggagcaagca aataacccac aatgacgaaa
     5581 gaggcagcaa ccgccacaat accccaatgt acagctaaca ggaagcccac tatgctacat
     5641 accgcattca aaaccattat gcccaactgc caggacggct tacctgcagc tttgattaca
     5701 cttccgttaa agaagagcac tgattgaaga atgccgatca atgacagcac ttgcataacc
     5761 ggaatacttg gtgtccattt ttcaccaaag actgttagaa ccaattcggg tgccaggatt
     5821 gccaatccaa aaaagatagg aaaagcaatt acgcttgtat attgtgtcac tttaaagaaa
     5881 gcctgacgca tccgttccgg ctgagcttga atacgggaaa atgcggggaa ggccacagag
     5941 tttgttacct ccgtgacaag ctttatgatg atcaacagga gttgataacc tatcgtataa
     6001 aaaccgagca gggttggtcc taaaaagtac ccgatcaaaa gatcatccga gcggcgcatc
     6061 agtacagtta gtgcatggct gccaacaatc ggtataccaa aactgacgag ttctttgtaa
     6121 tgcttctttg acactttcaa cccaggccgc caatcgcatg cctgccaaag gataatgacc
     6181 cgaactacac tggttgccag atcctgaccg acaagactcc ataccccata gcctgcaaac
     6241 gccatgccta cacctacaac ccctccgacc aaattggaaa ccgtcgagcg gatggcgaga
     6301 tctttgaaag acaggttgcg ttgtaagatc gacatttgtg tgccgctcaa cccgctcagt
     6361 agcagcgtaa tagatagcca cctcaggacc gctccgagct gcggttcatc aaacaacaca
     6421 gccaaccagt ctgaggcggc tatgaggact ccagtaagta caacgctcga tccaatattt
     6481 agccagaacg ctgtatcgag gtgctcagga tcaaggtctt tgcgttggac aatcgcggca
     6541 ccgaacccct gatccaggaa aagctcgatg aagactgtaa acgcagttgc cagagcgacc
     6601 aaaccaaacg cttctggggc cagcaaccgc gacaatataa cgaatgtgac cgttgtaacg
     6661 cctgccctcc cccacttttg tacaaacgac caaaatatgc ctttaactgc tttttgctgt
     6721 aaacccatgg tatcttctta ttctcttgct tttgtttatg gcagagccag tcggctctta
     6781 cgacccgttg ataatcggta taagtaaatc aatggccgat agaccataaa ttgtttcagc
     6841 cgggacgtgg ttaaattcca ttgattaaag ctgccttccc ttgtttgctg atagttaaca
     6901 agatcatttg caagcgaagg tccatattct ttttgccaga gcggaaataa ctcctgactg
     6961 acataatcat agagttgctg gtctacgcga ttcgcctcaa tcaacaattg gcgtgttttc
     7021 tcatccgaaa gaattttttg cgcgatcgag ttgttcgtgg caacgtttat ccgttggtaa
     7081 gaaatattca ggttcttcgc acgcaatccc ttcaacaaga gcatcgattc atcgaaacgc
     7141 tctgtaagtc cgataaatat attcttttct ttgataattc ggatagcggt atcaacatct
     7201 gcagatccag cgatcaactt ggtttggctg ttgcgcgtcc agtccctttg gatccattcc
     7261 tcgaagtcaa ggttttgctt gccacgatac tggatgttgt actgaaaacg cgaggcacag
     7321 gtttttatcg gatcacgcat gaaggcaaaa tacttgaatt cggtgccact ctcacctaaa
     7381 tctgtatagc ccgtcacgcg atgccccccg atactttcca agcgcggata aacttttcga
     7441 gaaagtttca ggtcatgagc cgagaacggt tgatctgaag aatcccgtgc gtgccagggt
     7501 tctgcttcgc agtggtagac gccaaacgag cttcgcagaa tatagcgaac cgtcctaccg
     7561 gcagttttat tgatgtgaat gaaaacaagc ataattatct ctcaaacgat ccagtcaaaa
     7621 tgaattttca gtgcgcttcg actcacaagc caccaaaact tcatccacaa ttttctcgat
     7681 gggagttttg ctggtgtcga attcaaggat ggtcggtccg ttgcgcattg acaaattggt
     7741 caacacatct ctaaaagaca tccgatattg cattaagtag ttccgtgctt ccagttcaga
     7801 ctttccctta atgccatgtt ttttcgtacg cacgttaatg cgctccatca gaatctcatc
     7861 ggatgtatcc agaaaaatga taacgtccaa cgcggctgcc cattgctcgt acaagtgttg
     7921 ccaccatgct ttagcttttt cgctccgaaa cgattccgga ccaaattcat ataatgtggc
     7981 catccggaaa acgggaccct gatccagtag aactgtcctg ctgttgctgg aaggaatccg
     8041 ctttaatact ttcgtccaac cttccaaata agcgatgtcc ttaagctctg tccaggttgg
     8101 ctgccgatcg gatttactct gatcaaaaaa caacggtaat gaagatggga tgctgcgaat
     8161 gaacacagga aggtgactta cttttcgaag ctcaatatcc ggtccaagca agatattgtc
     8221 gtcacgttgg cgtaaggctt tggaaagcgt cgtcttgccg gcgccagctg gaccaaccaa
     8281 ctccataatt aaaggagacc cggctttgag cttgtccgca actgctactt cattaatcat
     8341 agctgcgacc agatcaacgt ttttatttca gatgcgacct ctgccttgga cttgctcgca
     8401 tcaatgacat gaacatcggt gttttcccaa ctcaacttcc aaatttccgt agagcgagtt
     8461 cgaaccgact gtggatcctc gtcagttttt cgctgcactg ccacttccgg gtcgagtcgc
     8521 aataccatca atagttcggg caacaggatt tgtttgtagt aactctcttc taaccttatc
     8581 aaatatcttg caaaccaatg gttcaggcgc gggctcaaag gttggttggc tctggggccc
     8641 gcctgtagtt gacgaacgaa ttgctccgct tgcgcaccat ccatgatttg aatctgagga
     8701 agtggaaacc tatcaaggat gaccagcgaa ccctgggctg caagacggcg agctttgaca
     8761 tatgtccagt agcgatcgcg tgcccgacaa acctctcgga tcaagtacgg gtagcctggt
     8821 gaaacgcggg atttctgctg aattgtctcg tcaaacgtcg tctctaacgg ataaagacca
     8881 aggacttgtc cgatctttag aattgatcga atcgtaaccg tgatcggtga ccaggccggt
     8941 tttcccatgt ggatttttgt gattttaaag ttcttggaaa gccaggtaga gattgcccct
     9001 acggctgtag tcttgcctgc gccgtcccct cccaaaagag cgatgaccgc tccgccgatc
     9061 gcaagctgat acttggtgga aggtttgaag agtctgcgtt gaatgatcaa ggctgctcga
     9121 cgccacagct ttaaaaacgt gtcaacggga atggagtaga cggcattcga tcgaaggcgt
     9181 atctggagtt cttgcccggt tttcacacgt ttccagaggg aagcaccgga ttttaatgca
     9241 tctacgcatt ctttgaaaaa atcaacatca atataaggca gatattttct tagaatctgg
     9301 tttacatact gcgaatcact acgttcctgc aagtacacta gctctttacg ctcgctgctg
     9361 cttaattttc cttcccttcc aaggatcgca tcccaggtcg aatgtttgag taccattcga
     9421 atgacaagaa ttatcaactc aaactccaca gcaggaactc tgaagagctc gccttgtacc
     9481 gcagactcaa gatacggacg ttcgactgca agccgaaagt ttttggtcat gtcatggccc
     9541 atgatgagct gataatgagc atggacatgg acccattttt cggcatcatg atcgaaacca
     9601 aaaaagtcca atacacctgg catttgtttt tcaccgggag cagttacctg tttgaatccg
     9661 aggcgaaaca aaatttctgc aaatcgtgtt tcgtccgcgc gattgattaa caaatccagg
     9721 tcattatcgc catttgcgga ccgcgcaagc atgttattgc ttttccagtg gcagtaatca
     9781 atattttcaa ctctcagggc atcgcaaagc tttttaacaa taggcagcac tgcataattc
     9841 gtgggctgta ctgcgccttc cggacgaacc tgatcaagag tagaggaggt ctcaagggag
     9901 atcgtcatcg ttagttccaa ttaagcatca acaagctgat atacaactct ggcatttgtt
     9961 ttttgattct gaatggaaac atagaccgta tctgtagttt ccttggagga gctgatgatc
    10021 caggcagcgt gtgtacttct gctatagtca aagactttgt tccagcttga tccaccatca
    10081 tttgaataca tcaacaggca tctcgtacca ggtgcagaga agggcaggtt tgcccacatc
    10141 tcatatccct gttttgattt tctcatcacc atattatcaa tcggactccg gcgatagggg
    10201 tccggtacga tttttttcgt gaaggtcttg gcgtcggtgg tctcaacaat aaagttcgtc
    10261 ccaccctgat agtccgtgcc gaagaatact ttttcatcgt tttccacaac cgcggtatag
    10321 ccgcccatct ggatatgata tttcgtgatt ggaatccagt tggggttttt gaaatcaaac
    10381 gtgtctaagg gaccgcttat ccacagtttc ttatagttat ctccatccgc aaccatcaac
    10441 cggttcaaga ctttgctgta cttgacgatg tggacatgct tgttgatgcc tttctgaatg
    10501 aggaaatcag aaacatgcca ggtctcacca tcatcggcac tgtgatagag gtacgcaagt
    10561 tttctccagc cattcttttc caaaatgttg ccatattccc ccgcccacag ttcgccgctc
    10621 ggggtttcgg tcatggcgtt gttggagcgg aagaaactta tcggggaacc aaggtccagc
    10681 accttcttaa aggatttatt cttctcaaag cctcgataca tggatccttc aagacatacg
    10741 aagatcgtgt tttgactcga aataaacaga gacttaatgc tttctggaaa cttgtatata
    10801 aactcaaccg tcttaccacc atccacactc ttgtataaat cacagtcgtc ggggtataag
    10861 cttccaaaaa cctccccatt gttattaata tatatttgct tccaattgtg ttcgcatctg
    10921 gttgcgcgga ggcctttgct accgaaaaac cggcttacct tttcgtcaaa ttccagggtg
    10981 gctaactttt cgttctccag cgcaatcgca tctgctttgc tgaatgaaag ataatccttt
    11041 ttggggaata agccgaattt gtggtaagcc tcccaaacag cgaaggatac cagctcacga
    11101 aattttccga atttatagat atccttagct aaggaaaata tcttcttaac gccatttttg
    11161 gcaaaaaatc cggaatactg tttggttaga tcatgtccca tgattatctc acttatgact
    11221 tgatatcgat ttgaggataa ctggtgatta gaaaccgagt ttaccctcgt actactgacg
    11281 gaaacttatc agtctgacta actgattttt tgcattctcc gcctctgaca tttctcttgt
    11341 gtctgagtgg gcttgttttt tagacatcca gggcgctggc gtggataaac tgatatttct
    11401 taccagttac cagctctata tgattggagg aattttacag tctttctgga tgaattctgc
    11461 gtccatgacg atacggttaa gaaaaggtta caaaggcaat tacagtattg tatatacgac
    11521 caaggcttgt ttcgagaagg gttctacagc accgagttct tcttcggtgc tgtagaaaat
    11581 gtaaacagaa tagtgtagtc tattacggtc caatgtagtt ttggcgacga gattcgaagg
    11641 catcgaagaa gtaggtgcca ctcgttcccg catccatccc ggctaaggct cccagcctca
    11701 ctcggtcgat ccgcgaggtg tcgttgtcca cattcgttat attcgcctgc tgcacattat
    11761 cgatccacaa cgtgagccca ccattgttgg ctccggctcc cgttgccgct cgccagtcca
    11821 attcaatata gtgtggagca tcactgatgg taaaccagtt cgtgacgacg aaagaggatg
    11881 catcattcaa gatcttgccg cggatctgat atgctcccga cgagttgcgg agttccacct
    11941 gaagcacgtc ggtaccgctt cccagaaagc ctttgaagat gaagtgggca tccccattgg
    12001 ccatggtgat agagttcggg tcaaagtaga agcgagcgcg gtagcgtggc tcagcattag
    12061 gtgtgtcatc cgtgacgtag atcgtgttgg tatcatccac aactgcttgc agtccgtaat
    12121 tgccaaccag ggctgccgag ggactcacac tcagatcacc ggcatcgttg gaattggatg
    12181 tccaggcaga tagattgcca gactcaaatc catcagcaaa gatcacatcc gaaccaccgg
    12241 gcgtgtttgt ggctgttggg gtaaatgtga atgtcggcga gggtccggtt gtcggtgtgt
    12301 tggttggtgt gcgtgttgca gtggcggtcg gagtattcgt aaatgtcggt gagggtcctg
    12361 ttgtagctgt ggtggttggt gtagaggttt ttgttggggt attcgaagga gtagccgttg
    12421 ggacgggtcc ggtgaatgac aagttgaagc tatccagatc attcgccgcc agtccccagg
    12481 cacttccatc aaagaagagg gcagagaagt agctacaggc tgtcacactt ccaagtgaac
    12541 tcggtgcgca tacaaagaca tcttcgtcat ccccggaaac gcctgggacc gagaataagc
    12601 cggttgcaga tagatagata ttgccgctcg cgtcgatgtc aagggcatcc acatcctccg
    12661 agctaacatc gccaagtccc acatccgagc catcgaaata caatgtccat agcccggttg
    12721 tgttcgctcc cagggtagta ggcgtaaact gcaggatgtc ctcatcggca gcaccactaa
    12781 cacctgtcac agtggggttg ccggttgtgg agatcaacaa cctgccatct tgtaaaactt
    12841 caagcgcgtc aatattttca ctggaagcag tc
//
LOCUS       DASTJP010000217         5645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_984847, whole genome shotgun sequence.
ACCESSION   DASTJP010000217 DASTJP010000000
VERSION     DASTJP010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984847"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFQ23_06680"
     CDS             complement(<1..727)
                     /locus_tag="VFQ23_06680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HET9906305.1"
                     /translation="MMSKPKVFITRIIPDKGLDLIKDFCDVDVWTDDMPPGRADLLRR
                     VRGVDGLLSLLTEKIDEEVMKAAGSELKVISNFAVGFDNIDIQAATKRKIPVGNTPDV
                     LTDATADLAFALMLSVGRRLMEGQQYVRDGRWKTWSPTLLLGIEMKEATLGLIGFGRI
                     GKAVARRAMGFGMRVIYYDPTETYTHSDLNATRVDFETLLQEADFISLHTPLTEKTRH
                     IIDSAALSKMKPTAVLINTARGPI"
     gene            760..2052
                     /locus_tag="VFQ23_06685"
     CDS             760..2052
                     /locus_tag="VFQ23_06685"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET9906306.1"
                     /translation="MDVTRFATHSLKDPRILRVLSAALEAVDPFDVVQKELPEFERPV
                     FGLGIGKAAIPMMDALAAQVPLAGGLAVTKFAPRGTFRRYTVIEGGHPVPDARSLYAG
                     QEVLKFVSSLNEDDILICLISGGGSALVTAPDERISLEDLRSFTSLMLSCGARIDEIN
                     TIRRQLDRIKGGGLARAKKAKIISLILSDVIGNPLEAIASGPTVSDPTTSADALAILD
                     KYFATESPRYRDSDIINSVTEFLSGSEKIQPQDFDWSRIQNIIIGDNRLAAVAAMQQA
                     QHDGFHSDVLTNELQGEAREVGVRLARQLKEEVSNRSHPFCLVTGGETTVTIKGDGKG
                     GRNQELALAAVEELRDLQNAMLISLATDGDDGPTGAAGAVVTGESAQRAESLGLSAAE
                     YLSRNDAYPFFEALGDLLKTGPTGTNVNDLIFLIGGVV"
     gene            2049..2381
                     /locus_tag="VFQ23_06690"
     CDS             2049..2381
                     /locus_tag="VFQ23_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HET9906307.1"
                     /translation="MKTALILFFAVLSEVIATTSLKFSEGFTKLIPSIVVVVGYGLSF
                     YLLSMTLKIMPIGIAYAFWSGIGIVLTVIIGKIIWNESLDWARVTGILLIILGILIIN
                     LFSKTTAH"
     gene            2431..3003
                     /locus_tag="VFQ23_06695"
     CDS             2431..3003
                     /locus_tag="VFQ23_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9906308.1"
                     /translation="MVRSTNTKRAIMDLAEAFLQEKGFNGFSYAHIATALDVKNAAIH
                     YHFPTKESLTEAVIQRYRDRFQLWVNNARVKDLPPKEKLDWFFNIYTSTRADHGKVCL
                     AGSLETEFNSIPDSLRKQTQALTKELLSWLGATLSEGREAGVFRFSGNANGKAALILS
                     SLQGALQMARALGTAKFNEIVEQHKQDLLT"
     gene            3108..3917
                     /locus_tag="VFQ23_06700"
     CDS             3108..3917
                     /locus_tag="VFQ23_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9906309.1"
                     /translation="MNITEQTLSKRQAIIQRAYQAFFSPSKYELKSGDEGILARGHTY
                     RLPFEGGELAVTSWGDSGPTALLMHGWGGSRAQMTGFVDPLLSAGYRVVAYDQPAHGD
                     SDGKMTNMLKIAPTMDLIVKQEGKFDVVVAHSFGTLITSYALTKLNFPPPSRLVYFGA
                     FNRLMDSFPRFQVIAGLPDEIMDGFRSMLFENFGQDVLEAIVHEALAPQINIPALMFH
                     DIADNVTPVEDSRAIAQAWKGVRYIETEGLGHRGALQSPEIHEQVIRFLKS"
     gene            complement(3954..4886)
                     /locus_tag="VFQ23_06705"
     CDS             complement(3954..4886)
                     /locus_tag="VFQ23_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9906310.1"
                     /translation="MAEKLLDIFPEERAVAVLERIVPGSSLAKIELLPGSFSNHTHLV
                     EASLPNGAVYKIVVRRYKVFGEYDRGEKANREFKTFELLNRHKVPAPEPLLLDVSGDV
                     LGSPGIVAEFVEGHLKMDVPSDPMEWSRKLARTLAKIHSIPCSENEQSFLLNGNEQAS
                     WFLNYDSAPHYMQEYPGGADLWSEMRRRSSKLVTDGPSLLHIDYWSGNILWLENEISA
                     VLDWEEASYGDPAVDVGYALMNIVLMDAPDAADEFLSVYEAEMGRQIKNLVFWELAAS
                     VRPMTDPVDWNVHETGSNKDVLLEFIENAKRRAE"
     gene            4970..>5645
                     /locus_tag="VFQ23_06710"
     CDS             4970..>5645
                     /locus_tag="VFQ23_06710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9906311.1"
                     /translation="MTQSTLQKAWSRIKPDFLWWFVIGLGILLRVRQYLVNRSFWADE
                     ASLAFNLANRTFSGLAQPLDYHQGAPIGFLLIEKLAILLFGNNEYAMRLFPLIAGIIA
                     IYLLYLLAKTYIGLSGVFALLAFSVGWYLIYYASELKQYSSDVMIALLLVYLASKCIQ
                     EQAQARDFVVLGIIGAIAIWVSHPSVFVLAGVGLVLVIDKVTRKSEIPFAWILIMGLL
                     WVGLFAI"
BASE COUNT         1405 a         1387 c         1371 g         1482 t
ORIGIN      
        1 cgatcggtcc acgcgcagta ttgatcagca ctgctgtagg cttcatcttc gaaagggcag
       61 cggagtcaat gatgtggcgt gtcttctctg tcagcggcgt atgaagtgaa ataaaatcgg
      121 cctcttgtag aagcgtttca aaatcgactc tcgttgcatt gagatccgaa tgcgtgtatg
      181 tttctgtagg gtcataatag atcactcgca taccaaaccc catggctcgg cgtgccactg
      241 ctttgccgat gcgtccaaag ccgatcagtc caagcgtagc ttccttcatt tcgattccaa
      301 gcagtagcgt gggactccag gtcttccaac ggccgtcgcg cacatactgc tggccttcca
      361 tcaggcggcg accaacactt agcatcaagg caaaagccaa atcggctgtt gcatcggtca
      421 gcacgtcggg tgtgttccca accggaattt ttcgcttggt agcagcctga atgtcgatgt
      481 tatcgaagcc gacagcgaaa ttgctgatca ctttcaattc tgaccccgct gccttcatta
      541 cttcttcgtc tattttctcc gtgagcaggg ataataaacc gtccacaccg cgcacacgcc
      601 gcaatagatc cgctcgacct ggcggcatgt catcagtcca tacatctaca tcgcaaaagt
      661 ctttgatcag gtcaagtccc ttatcgggaa tgatccttgt gataaatact ttgggtttcg
      721 acatcattgc tccgatttgt ttctgttatg ataacagaca tggatgttac tcgcttcgcc
      781 acgcacagtt tgaaagaccc gcgcatttta cgggtcttat ctgcggcgct tgaggctgtt
      841 gacccgtttg acgtggttca aaaagagctt ccagaatttg agcgtcccgt tttcggatta
      901 ggtattggga aagctgcaat cccgatgatg gatgcgctcg ccgctcaggt cccactggca
      961 ggtgggcttg ccgttaccaa attcgctccg cgcgggacat tcaggcgtta caccgttatt
     1021 gaaggcgggc acccggtccc ggacgcgcgt tcattgtatg caggtcagga ggtattaaag
     1081 tttgtttctt cgctgaatga ggatgacatt ttaatctgtc tcatctcagg tggcggttct
     1141 gctttggtga cggctcccga tgaacgcatc tcgcttgaag atttaagatc gtttacttca
     1201 ttaatgcttt cctgtggcgc gcgcattgac gagatcaata ccattcgccg ccaactggat
     1261 cgtatcaagg gtgggggatt ggcgcgtgca aaaaaagcaa aaatcatcag tttgattttg
     1321 tctgacgtga ttggcaatcc gcttgaagcg attgcttccg gaccaactgt ctccgaccca
     1381 acaacatcag cagatgcgct cgccattttg gacaagtact tcgctacaga atcaccgagg
     1441 tacagagatt cagatatcat caactccgtg actgagtttc tcagtggttc agaaaaaatt
     1501 cagccgcagg attttgattg gtctcgcata caaaacatca tcatcggcga taataggctg
     1561 gcggcagtag ctgccatgca gcaggcacaa cacgacggtt ttcattcaga tgttctaaca
     1621 aatgaactac agggtgaagc gcgagaggtc ggggtgaggc tcgccagaca actgaaagaa
     1681 gaagtctcca atcgctctca tccattctgt ttggtcacag gtggcgagac aactgtgact
     1741 atcaaaggcg acggcaaagg cggaagaaat caggaactgg cattggcggc agtggaagaa
     1801 ctgagagatt tgcagaacgc catgttgatt tctcttgcaa cagatggcga tgacggtccc
     1861 acaggtgcag caggcgcggt agtgaccggt gagtctgcgc aacgcgccga gtcgcttggt
     1921 cttagcgcgg cagagtattt atcccgaaat gatgcatatc cgtttttcga ggcgctgggt
     1981 gatctcctca agacaggtcc taccgggacg aacgtcaacg accttatttt cttaattgga
     2041 ggtgtggtat gaaaacggcg ttgatcctct ttttcgcggt tcttagtgaa gtgatcgcaa
     2101 caacatcgct gaaattttct gaagggttta cgaaactcat tcccagcatc gtagtagtgg
     2161 tagggtatgg gctatcgttc tatttacttt ccatgacact gaagataatg ccgatcggca
     2221 tagcatacgc gttctggtct ggcattggca tcgtattgac cgtgattatc gggaaaatca
     2281 tttggaacga atcgttggac tgggctcggg tgaccggcat cctcctgatt attttgggga
     2341 ttttgattat caacctattt tctaaaacta cagctcatta gaaataccag acccattgac
     2401 aacttactaa ctagtaagta aaatattctc atggttcgaa gtacgaatac caaacgcgca
     2461 attatggatc ttgcagaggc cttcttacag gaaaaaggct tcaatggctt tagctatgct
     2521 cacatcgcta ccgctctgga tgtcaaaaac gcggccattc attaccattt cccgaccaaa
     2581 gagtccctta cggaggctgt cattcaacgc tatcgagacc gcttccagct ctgggtcaat
     2641 aatgcacgtg taaaagattt gcctcccaaa gagaagctcg attggttttt taacatctac
     2701 accagtacgc gggcagatca tggcaaggtt tgcctcgctg gttcgctgga aaccgaattc
     2761 aattccattc cagactcatt gcgcaagcag acgcaagcat tgacgaagga attgctctcc
     2821 tggttgggcg cgactttaag tgaaggaaga gaagctggcg tttttcgttt cagcggtaat
     2881 gctaatggca aagcggcgtt gatcctttcc agtttacagg gcgccttaca gatggcacga
     2941 gcgctcggca ctgcgaagtt caatgagatt gtggagcaac acaaacagga tctgcttaca
     3001 taaaattttt ttagtcttac aaacttacta cttagtcagt atcaagatta caaatatgca
     3061 gcgactgaag ttgctactac aacaactgat tatcaaaagg agaaaacatg aacataacgg
     3121 agcaaactct gtccaaacga caggcaatca ttcagagagc ataccaggca tttttcagcc
     3181 catccaaata tgaattgaaa tcaggggatg aagggattct cgcacgcggg cacacctatc
     3241 gcctcccgtt tgaaggaggt gagttagccg ttacaagctg gggagattcc ggcccgaccg
     3301 ccctgctgat gcatggatgg ggtggaagcc gcgcgcagat gacagggttt gtcgatccgt
     3361 tgctatcagc aggatatcgc gtggtggcgt acgatcaacc tgctcatggg gattcagatg
     3421 gaaaaatgac caacatgctg aaaattgcgc ccacgatgga tttgatcgtc aagcaggaag
     3481 gaaaatttga tgttgtcgtg gcccattcat tcgggactct aattacttca tacgccctaa
     3541 ccaaattgaa tttcccacct ccatcacgac ttgtctattt cggcgcgttc aatcggctga
     3601 tggactcgtt cccccgcttt caggtgatcg cgggtttgcc agatgagatc atggatggat
     3661 ttcgaagtat gctttttgaa aatttcgggc aggatgtgct ggaagccatt gtccacgaag
     3721 cgctggctcc ccaaattaat attcccgcgc tcatgttcca cgacattgca gataacgtca
     3781 cgccggtgga agacagccgc gccatcgcac aagcctggaa aggtgtacgg tacatcgaaa
     3841 cggaaggatt gggacatcga ggcgccctac aatcaccgga gatccatgag caagtgatac
     3901 ggttcctgaa gtcgtaaaac ataaatctcc ctcacaatga agtgagggag attttattcg
     3961 gcccgccttt ttgcgttctc gataaattcc agaagaacat ccttgttcga ccctgtttca
     4021 tgtacgttcc aatccacggg atcggtcatc ggtcggacgg atgcagccag ttcccagaaa
     4081 accagatttt taatctggcg tcccatttca gcttcgtaga cgcttaagaa ttcatctgcc
     4141 gcgtcgggcg cgtccatcag aacgatattc atgagcgcat accccacatc aaccgctggg
     4201 tcgccatacg aagcctcctc ccaatcgagc acagcggaga tttcgttctc cagccacaaa
     4261 atatttccag accaataatc gatgtgtaaa agcgatggcc catctgtgac tagttttgac
     4321 gaacgcctgc gcatctcaga ccataaatcc gcgccacctg gatactcctg catataatga
     4381 ggggctgaat catagttcaa gaaccatgag gcttgttcgt tgccgttcaa gagaaagctc
     4441 tgttcgttct cactgcaagg aatggaatga attttcgcca atgtcctggc gagcttcctt
     4501 gaccactcca taggatctga cggcacatcc atcttcagat gcccctccac aaactccgcg
     4561 acgatcccag gggagcctaa cacatcaccg gacacatcca gcagcagcgg ctcgggcgca
     4621 gggaccttat gcctgttcag caactcgaag gttttaaact ctctgttggc tttttcaccg
     4681 cgatcatact ctccgaatac tttatagcgt cggacgacga tcttatacac tgccccattc
     4741 ggcaaactcg cttctaccag gtgagtgtga tttgaaaagc ttccgggaag taattctatt
     4801 ttggctaatg aactgcctgg cacgatcctc tccaggacgg caacggctcg ttcttcagga
     4861 aaaatatcca gtaatttctc tgccatgtaa aaaattatat cagccgtcaa aatccaaaac
     4921 tcgcccagtg ttagaattat ccactgacag gttgtcacct ggatttccca tgactcagag
     4981 tacgctacaa aaagcctggt ctcgaataaa accagatttc ctctggtggt ttgtcattgg
     5041 tcttgggatt ctcttgcgcg tgcggcaata tcttgtaaac cgctccttct gggcagatga
     5101 ggcaagcctt gcgtttaatt tggcaaatcg cacttttagc ggtcttgctc agcccctgga
     5161 ctaccatcag ggcgcaccca ttggcttcct attaattgag aagcttgcca ttctgttgtt
     5221 cggaaataac gaatacgcga tgcgcctctt cccgctgatc gccgggataa tcgctattta
     5281 tttactttat ctcctcgcga aaacctatat tggattaagc ggagtatttg ccctgctcgc
     5341 attttccgtc ggatggtatc tgatctatta tgcctccgaa cttaagcaat attcgagcga
     5401 tgtcatgatc gcgttactat tggtttatct ggcaagtaaa tgcattcagg aacaggctca
     5461 ggcaagagat ttcgttgttt tgggtatcat tggggcaatt gcaatatggg tatctcaccc
     5521 atcggttttt gtgctggccg gagtcggatt ggtgctggtc atcgataaag tgacacgaaa
     5581 aagtgaaatt ccgtttgcgt ggattcttat catgggcttg ctttgggtcg gtctatttgc
     5641 gatag
//
LOCUS       DASTJP010000218        10397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_618415, whole genome shotgun sequence.
ACCESSION   DASTJP010000218 DASTJP010000000
VERSION     DASTJP010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10397
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618415"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..548
                     /locus_tag="VFQ23_06715"
     CDS             99..548
                     /locus_tag="VFQ23_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906312.1"
                     /translation="MKTQTQFFMIAGLVILILAVGWLGYSTRNQEPAHVFAATINRDC
                     APWDGGAFTVSIPVDVTTTIMISIWQSPDIQHPIAFTFPDESGQTGFVYILPEIDPLQ
                     PLSGEVFFGRVEQGVPVEGEFNLTTERGEQYHGRFRAVWGDQVVYCG"
     gene            642..1484
                     /locus_tag="VFQ23_06720"
     CDS             642..1484
                     /locus_tag="VFQ23_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HET9906313.1"
                     /translation="MDNDDKPIGQLLSRRDALKLLGLGSAAFLAACAAPEGTSAVVPT
                     SVSTLASSTQASSVTTTALDCVVRPEMTIGPYFVDDQLNRSDIRSEPSDNSVRAGLPL
                     TLAIGVFDVANNSCTPITNAQVDVWHCDADGVYSGVSDQGFDTTGQKFLRGYQLTDAT
                     GKVQFLTIYPGWYSGRTVHIHFTIRTTGADGNDYQFTSQFFFDDALTDQVYPLEPYAG
                     RGERNTRNSDDNIYNNGGDQLLLNLQGDTTNGYTTAINIGLDLTDTGVGAADSMGGPG
                     GGPP"
     gene            1570..2190
                     /locus_tag="VFQ23_06725"
     CDS             1570..2190
                     /locus_tag="VFQ23_06725"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9906314.1"
                     /translation="MTENAGYRTLTLTDLEQMVHVLSTAFEEDPLVSFILPKKETRVK
                     TVAKFFRAMGRLNIQNKLAFGVSDPLEGVAVWNFPDKPNISATLKDLIAFLPLLFSSY
                     PLGAQRARAIFRQLDTMHKKYASEPHFYLDNLGVLPSARGKGLSSQLIRPFLEMADSQ
                     KVVAYTDTVTPSNAPLYEHFGFQCVEETHIPSTGITVFALRRPIRA"
     gene            2202..2510
                     /locus_tag="VFQ23_06730"
     CDS             2202..2510
                     /locus_tag="VFQ23_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HET9906315.1"
                     /translation="MPDQKSVLSKLEQIESEMLRIGMWQKTPLEPEQYDFRAAFAGDT
                     MTFSQWLQFIFIPNVRSAAASGKFPASSQVAAQAIRELDGVDEASGLITLISEFDALF
                     "
     gene            2525..3421
                     /locus_tag="VFQ23_06735"
     CDS             2525..3421
                     /locus_tag="VFQ23_06735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906316.1"
                     /translation="MKNIDRVKRHAAENGRLAYQLKNANVYVSADGFHFIDYLDPVSE
                     ISPEIDASQLTKEESSYIETQLQANTHKFENQANLLKKHLPPKDAKVLDIGCGGGLFL
                     SLLKREGAEVTGIELSDSRAQYAKTKHNLEILKYPIESEYWQKDFAGHFDAATLWDVI
                     EHVNYPHQTLQSAVNVLKPGGLLLIDTPCRDSFYHQFGELTYRLSGGRFPTFLNAMYS
                     SHLFGHKQIFSTKEMKELFESVGLEVIDLHKFHELSFPFDFYLRKLFKSEIIVKIMLP
                     LVRLFFRIFKIQNKMLVVGRKA"
     gene            3644..4408
                     /locus_tag="VFQ23_06740"
     CDS             3644..4408
                     /locus_tag="VFQ23_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET9906317.1"
                     /translation="MNNNDYIPQRAMSIHAHPDDQEFSVGGTLAKWAKAGCEVISVVI
                     TSGDSGSNDPAKDGSHKQELAELREQEQLAANEILGVRETIFLRYPDGELEPTIQLRK
                     DLARLIRQYKPDTVTAGNPEAWFYGDEYLNHPDHRAAAQAACEAVFPSAGSRLMFVDL
                     LAAGYEPHDVKRLYIHGIEKANTWVDITETMDVKIKALQQHVSQIDPNEVGQWMMEWA
                     EHEAKDQEMKYAESYKVMMLKRDEEEKLEEKPPHED"
     gene            complement(4379..5080)
                     /locus_tag="VFQ23_06745"
     CDS             complement(4379..5080)
                     /locus_tag="VFQ23_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9906318.1"
                     /translation="MNPYLATLFTFLIALAFLRLMDFLAHRGIIESKLSRKLIHIGTG
                     PIFVLCWLMFPDLPISRWLAALVPLVITTQFVLVGTGILKDEAAVKAMSRTGDRSEIL
                     RGPLFYGIVFILMTVIYWKDSPIGIPALMMMCGGDGIADIIGRRVSSPKLPWSREKSV
                     AGSLSVFAGGWLLTVLIFAIYIWAGAFDGPLSLYILPITWIAAGAMLVESLPYKDIDN
                     VTIALASVLVGGFFF"
     gene            complement(5168..5314)
                     /locus_tag="VFQ23_06750"
     CDS             complement(5168..5314)
                     /locus_tag="VFQ23_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906319.1"
                     /translation="MKLTKEDFKRFVKYWRVAYKQANSEDRLSLGMFGLLMLVCTWWV
                     ILVA"
     gene            5501..5683
                     /locus_tag="VFQ23_06755"
     CDS             5501..5683
                     /locus_tag="VFQ23_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906320.1"
                     /translation="MQKWEYQIVKMESLSLQEQGKTLEALGAHGWELVTVTAIVGIPE
                     KPATLCFYLKRLSYPK"
     gene            complement(5719..7623)
                     /locus_tag="VFQ23_06760"
     CDS             complement(5719..7623)
                     /locus_tag="VFQ23_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906321.1"
                     /translation="MKANVSARGYTTVILIINMKRFITQEDDERKRFETLRGKRDFAN
                     VLRTVWPEQVRKQDIFTTMEDFRKRNISEAGKQLETGDIIATDQIGFATGVFRSLMLE
                     EYSFPAYAVDKSRIQFSPDLETNFQFKTLFKRVWDRWEIYIRPTFSGFFVIRLTNRYQ
                     EKTRSLLAIAQDVLRLQESIDVLSAQKWLEHSRERYFNDPDTLDIKERSVKALLDWVG
                     GNDHKGELLYYPIQWKIAMEVASRFVELIGSEIPMPEAKPINLVRPDPSLSIPLHDSY
                     VIHHFDDVLAEPSLFKKPNGKTKSNAQSIVSLNDIRQSHILKRTLVNLIEGSLLRSQS
                     IHSSKATDVLNTQTIESTSYFPNPKWSLADNLFELNQASWNDEFCLLGARTALIIPSQ
                     EWKNSEISVSTVPSSTLHVKYLRYWGAIERMVEFVIEIRVLSQLIESESYDLLREIAQ
                     TVHETRSKLFTGDIKLDQRLPDLVTRAAYLRHLAALSQSLSHPQLWSRAEYAIQKATY
                     LLEQLGVPTILEHIERNITSISSVVDHVDELYLADLSEKENDNSTILSLGLAAASLTL
                     TLLMLPSFWADIKSSWGDPFTVWQWMLAAFGIVLSICLIAGALYMLRIAFIQRKKVKK
                     MFDKFLEGTS"
     gene            complement(8378..9784)
                     /locus_tag="VFQ23_06765"
     CDS             complement(8378..9784)
                     /locus_tag="VFQ23_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906322.1"
                     /translation="MNKLLIKLAIGLALIFFVLPEFMPAPNLGVTTVQAADSGNNDDK
                     DKKNKCKKKNGERKKKCKNKNDPDNAENSNGPVDPATFFIPHMPETLNNPLEALAWVN
                     EHEEACNIEGANGPDPYGVATFTDGQGHTYTTPNFWLDVWDSETLNVPDIENWGETIY
                     YTVTFYFPSRDEHQYDTDTGDFSNKCRIVKTPPTQLVRHKKVIAAPDWCPLQPYLRAT
                     KVDDRSYIEWRDGRTIELPLVQNMWEFEIGDFINPSIHWTNCKYATEYWNEDGNVSVR
                     IYDFGGTLLKEYEGMKMPEFPLKESSPLLFAIREADGQLMEINIETDRMMGKGAFGIN
                     PISVVTMQGIYVALTVEGNLLSVVESTGKVNQTEQVCQRVEADHQKRLFACRTDDNIV
                     YYNPETGNTTEIQNTWDAAAINPENSQQIALTGLNLSVLNGSELSEVSELRSYGGSDW
                     GGKTHETVRILDFLQNLK"
BASE COUNT         2762 a         2463 c         2472 g         2700 t
ORIGIN      
        1 tcgtctgcgt tcgggcgaca atcacgctct cgttccgctc agcgcgaaca cgtttctagg
       61 aggaacattc aaaacgaacg gagcgtcctg ctttcagtat gaaaactcaa acacagtttt
      121 ttatgatcgc tggtctggtc attcttattc tcgctgtcgg ctggcttggc tattcaacgc
      181 gaaatcagga gcccgcacac gtctttgcag ccactataaa tcgggactgc gcaccgtggg
      241 atggaggcgc gtttacggtc tcgattcccg ttgatgtaac tacaacaatt atgatttcca
      301 tctggcaatc gccagatatt caacacccga ttgcatttac atttcccgat gaatccggac
      361 aaactggctt tgtttacatt ctgccagaaa ttgatccact tcaaccattg agcggggagg
      421 ttttcttcgg gcgcgttgag cagggggtgc ccgtcgaagg tgagttcaac ctcacgacag
      481 aacgaggtga acaatatcat gggcggttca gagctgtgtg gggagatcaa gtggtgtatt
      541 gcgggtagcg tcctcacaat atttacataa tttcttcatc tactcctaaa gcctccccag
      601 tatcatgaac gaaacaaatt cgtatcactc aggagatgat tatggataac gatgataaac
      661 ctattgggca gcttctctcg cgaagagatg cgctcaaatt gttagggctt ggcagcgcgg
      721 cattccttgc cgcatgtgct gcacctgaag ggacgagcgc agttgtccca acctctgtat
      781 cgacgctcgc ctcctccaca caggcgtctt cggtgacgac taccgcgttg gattgcgtgg
      841 tacggcctga aatgaccatc ggaccgtact ttgtagatga ccagctcaac cgatctgaca
      901 tccgctccga accatccgat aactctgtca gagcaggctt accgcttaca cttgccatcg
      961 gagtctttga tgttgccaat aatagttgta cgccgatcac aaacgcgcaa gtggacgtct
     1021 ggcactgcga cgcagatggt gtgtattccg gtgtttctga tcaggggttt gatactaccg
     1081 ggcaaaagtt cctgcgcggc tatcagttga ccgatgccac tggcaaagtg caatttctga
     1141 cgatctaccc tggctggtat tcgggcagaa cggtacatat ccatttcacg atccgcacga
     1201 ccggcgcgga tggcaatgat tatcaattta cctcccagtt tttctttgac gatgcattga
     1261 ctgatcaagt ctatcccctt gaaccgtacg caggcagagg cgaacgcaat acaagaaata
     1321 gcgacgataa tatctataat aacggtggtg accaattatt gctcaacctg caaggggata
     1381 ctaccaatgg ctatactacc gccattaata tcgggctgga tctcacggat acaggagttg
     1441 gagccgccga ctcgatgggt ggtcccggcg gaggacctcc ataagaagtc ctataatcca
     1501 caaactattc atacaaacat ccgaagtcta tagcacttcg gatgtttact ttcagttaca
     1561 atcagccgcg tgacggagaa cgctggttac cgaactctta cgctgaccga ccttgagcag
     1621 atggttcatg tcctgtctac ggcctttgaa gaggatccgt tggtttcatt cattctcccg
     1681 aagaaagaga cgcgcgtgaa aaccgttgcg aaattcttcc gtgccatggg caggctaaat
     1741 atccaaaaca agctggcttt tggtgtgtct gacccgctgg agggcgtcgc tgtctggaac
     1801 ttcccagaca agccgaatat ttctgcaacc ctcaaagatc tgatagcttt ccttccactt
     1861 cttttttctt cctatccatt gggtgcacaa cgggcgcgag ccatcttcag acagttggat
     1921 acaatgcata agaaatatgc gtcagaacct catttttatc tggataatct tggtgtattg
     1981 ccatccgctc gtggcaaggg actttcgtct cagttaatcc gcccatttct ggaaatggct
     2041 gattcgcaaa aagtagttgc ctataccgat actgtcaccc cttcgaatgc cccgctgtat
     2101 gaacatttcg gatttcagtg cgtggaagag acgcatattc caagtacggg tatcaccgtc
     2161 tttgcactgc gaagaccgat tcgagcctaa aggaaaaagc cgtgcctgat caaaagtctg
     2221 tcttatcgaa actggagcag atcgaatctg aaatgctccg cattggtatg tggcagaaaa
     2281 cgccactcga gccggaacaa tatgatttca gagccgcatt tgccggggat accatgacat
     2341 tttctcagtg gctgcaattt atcttcatcc ccaatgtacg aagcgcggcg gccagcggga
     2401 agttccccgc cagcagccag gttgccgcac aggcgatccg tgaattggac ggggttgacg
     2461 aagcttcagg tcttattact ctcattagcg aattcgacgc gttgttttag ggaactcaat
     2521 tcctgtgaag aatattgaca gagtaaaaag gcatgccgct gaaaacggac ggcttgccta
     2581 tcaactgaaa aatgcaaatg tctacgtctc tgcagatgga tttcacttca ttgactatct
     2641 ggatccggta tccgagattt cccctgaaat cgatgcgtct caattaacaa aagaagaatc
     2701 atcctacatc gaaacccagc tacaggcgaa tacgcacaaa ttcgagaatc aagccaacct
     2761 cctcaaaaag cacctgcctc cgaaagatgc gaaggttttg gatatcggct gcggtggggg
     2821 attgttcctg tcacttttga agcgggaagg tgccgaagtg accggcatcg aactcagcga
     2881 cagccgcgcg caatatgcaa agacaaagca caatctcgaa atccttaaat atcccatcga
     2941 aagtgaatac tggcagaagg attttgcggg tcatttcgat gcagccacct tatgggatgt
     3001 gatcgagcat gtcaattatc cacatcaaac tctccagtct gctgtgaatg tcttgaagcc
     3061 aggtggcctg ttgctgattg acacgccatg ccgagacagt ttctatcacc agttcggcga
     3121 acttacgtac cggttgtcag gaggcaggtt tccgacattc ctgaacgcga tgtattcgtc
     3181 gcatttgttt ggtcataagc agatcttttc gacgaaagag atgaaagaat tattcgagtc
     3241 agtgggtctt gaagtcattg atctgcataa gttccatgag ctttcgttcc catttgattt
     3301 ctaccttcga aagcttttca aatcagagat catcgttaaa ataatgctgc ccctggtcag
     3361 gctatttttc aggatattta aaattcaaaa taaaatgctg gtggttggga gaaaggcata
     3421 atcatgtcat tgcgagcgac cgctggtcga gtagggctga gcgtttgttg cgaagcccat
     3481 atcgagacca cgacaacgaa gcaacacctg cactccccgg catcgaccaa ccggagcggt
     3541 gtgcaggcag gtgcaagtgt ctcaactaat ctgcaatttt ttcgttgcat gaaatcacga
     3601 gattgccacg tcgcaatgac gtagatctaa taggagaacc cttatgaaca acaacgatta
     3661 cattccgcaa cgagcgatga gcatccatgc gcacccggac gatcaggaat tttccgtcgg
     3721 tgggacgctg gcgaaatggg cgaaagccgg ctgtgaggtc atctcggtgg tcatcaccag
     3781 cggcgactca ggctcaaatg acccggcaaa agacggcagt cataagcagg aattggctga
     3841 gttacgtgag caggaacagc ttgcggcgaa tgagattttg ggagtcagag agacgatctt
     3901 cctgcgttac ccggatggtg agctggaacc gacgattcaa ctccgcaagg atctcgcacg
     3961 cctcatccga cagtacaagc cggataccgt tacggcgggc aaccccgaag cctggttcta
     4021 tggtgacgaa tacctcaatc accctgatca tcgcgctgcg gcacaagccg cctgcgaggc
     4081 cgtattccca tccgcgggga gccggctcat gttcgtggac ctgctcgcgg cggggtatga
     4141 accgcatgac gtcaagcgac tgtacatcca tgggatcgag aaagctaata cgtgggtcga
     4201 tatcactgag accatggacg tcaagatcaa agccttacag cagcacgtga gtcagatcga
     4261 ccccaacgag gtggggcagt ggatgatgga atgggcggag cacgaagcca aggatcagga
     4321 aatgaagtat gctgagagtt ataaagtgat gatgctgaaa cgcgacgaag aggaaaagtt
     4381 agaagaaaaa cccccccacg aggactgaag ccagcgcgat cgtgacattg tcaatgtctt
     4441 tgtacggcaa tgattcgacc agcattgctc ctgctgcgat ccatgtgatg ggcaggatgt
     4501 agaggctgag tgggccatcg aacgctcctg cccaaatgta aatggcaaag ataaggacag
     4561 tcaataacca gccgcctgcg aacacactca aagaaccagc cacgcttttt tcgcgcgacc
     4621 agggaagctt cggtgaagag acgcgtcgtc caataatatc cgcaatgccg tcgcccccac
     4681 acatcatcat caacgccggg attccgatgg gcgaatcttt ccagtagatc acggtcatca
     4741 ggataaacac aattccataa aacagcggac cgcgcaggat ctcgcttcga tcccctgtgc
     4801 gcgacatggc tttgacagct gcttcatctt tcagtatgcc tgttcccacc agaacaaatt
     4861 gtgtcgtgat cacaagcgga accagtgccg ctaaccagcg agaaatgggc aggtcgggga
     4921 acatcaacca gcacaaaaca aaaatcggac ctgtgccgat gtgaattaat ttccgactta
     4981 atttactttc aatgatgcct cgatgcgcaa ggaagtccat caggcgcagg aaggcaaggg
     5041 caatgaggaa ggtgaagagg gtggcgaggt agggattcat gttctgtatt aacacaaaat
     5101 gccgcgtttc agcgcggcac aattatagag cgtcagtata aatttacagc gtggtggtcg
     5161 gaaggtatca ggctaccaga atgacccacc aggtgcaaac cagcatcagc agaccaaaca
     5221 ttcccaggga caggcgatcc tcactgtttg cctgcttata cgctacgcgc cagtatttca
     5281 caaatcgttt gaaatcttct tttgtcagtt tcataagatg cattatcttc ttaagaaata
     5341 gacgcaaatc acgggaaaaa tgtatcagcc tcccgaaatc aattctttcc cccatcggta
     5401 ccgcttccaa tgcagaacgt ctcacatcca ggcggcacac tagaaaataa atatataatc
     5461 ccttctggta ccctggtagc tgcataacac ggaggatcca atgcaaaaat gggaatatca
     5521 gatcgtcaaa atggagtcgc ttagccttca ggaacaaggg aaaaccctgg aagcactggg
     5581 agcgcacggg tgggaattgg tcactgttac cgcaattgtt ggaattcctg aaaagcctgc
     5641 aacgttgtgt ttttatctca aacgtcttag ttatccaaaa tgaagccgag ggtcggctgg
     5701 aacgatgtgt actgaagcct aggacgtgcc ttccaggaat ttatcaaaca tcttcttgac
     5761 ctttttacgt tgaataaatg caatgcgaag catataaagt gccccggcaa taagacaaat
     5821 agaaagcacg attccaaacg cggctaacat ccattgccag accgtaaagg gatctcccca
     5881 actggatttg atgtctgccc agaaagaggg caacatgagc agcgttaagg taagcgatgc
     5941 tgcagctaat cccagggata gtatggtgga gttatcattt tccttctctg agagatcggc
     6001 caggtaaagc tcatccacat gatcgacaac actgctgatg ctggtaatat ttctttcgat
     6061 gtgctccaga attgttggga cgcctaactg ctccaaaagg taggtcgcct tttgtattgc
     6121 gtattctgca cgactccaca attgagggtg gctcaaactt tgagagagcg ctgccaggtg
     6181 tctcaaataa gcagcccggg taaccagatc aggcagtcgc tggtccaact taatgtctcc
     6241 tgtaaatagc ttggaacgtg tttcatgaac cgtttgcgcg atttctctta agagatcgta
     6301 agactcgctt tcaatcaatt gcgacaatac tctgatttct attacgaact ccaccattct
     6361 ctcgattgcg ccccaatatc tcaaatattt tacatgcaac gtggagctag gcacggtgga
     6421 aactgaaatt tcactgtttt tccactcctg cgaaggaata atcaacgccg ttcttgcacc
     6481 caacaaacaa aactcgtcat tccaggatgc ctgatttaac tcaaatagat tgtctgccag
     6541 gctccacttt gggttgggaa aataactcgt ggactcgatc gtttgggtgt tgagaacatc
     6601 tgttgcttta ctggaatgga tggactgaga tctcaacagg gaaccttcga tgaggttgac
     6661 aagcgttcgt ttgagaatat gagactgtcg gatatcgttc aacgatacaa tactctgagc
     6721 attgcttttc gttttaccgt ttggcttctt aaagagcgaa ggctcagcaa ggacatcatc
     6781 aaagtgatgg atcacatacg agtcatgtaa tgggatggag agagatggat ctggacgtac
     6841 cagattaatg ggcttggctt ctggcattgg aatttcagaa ccaatcaatt ccacaaatct
     6901 ggaggctact tccattgcaa ttttccactg aatcggataa taaagcagct cgcccttatg
     6961 gtcatttcca cccacccaat ccaaaagtgc tttaacagac ctttccttta tatccagcgt
     7021 atctgggtca ttgaaatatc tttccctgga atgttcaagc catttttgcg cgctgagcac
     7081 atcgatagac tcttgtaatc gtagtacatc ttgcgcaatg gcaagcagcg agcgtgtctt
     7141 ctcctggtag cgatttgtca ggcgaatcac aaagaaaccc gaaaacgtgg gtcgaatata
     7201 aatctcccag cgatcccaaa ctctcttgaa caacgttttg aactggaaat tggtttcaag
     7261 gtccggagaa aattgaatgc gagacttatc cactgcataa gcagggaagc tgtattcttc
     7321 cagcattagg gagcggaaga cgcctgttgc aaatccgatt tgatcggtag caataatgtc
     7381 accagtctcc agttgttttc ccgcttcgct gatatttcgc tttctaaaat cttccatagt
     7441 ggtaaagatg tcctgcttcc ggacctgctc cggccatacc gttcgaagta cattcgcaaa
     7501 gtccctcttg cctctcaatg tttcaaatcg tttacgctca tcgtcttcct gcgtgataaa
     7561 ccgcttcata ttaataatta agatgacagt ggtatagcct cttgctgata catttgcttt
     7621 cataagaatc ctccatgagt cgaaagcaga caccaggcaa aacttgacct gctggaattc
     7681 gagttggtgt gcgggttgaa ttaaattaat tcgaacggat tgataatttt acttgaattt
     7741 cagaagtaaa agactttatc gcgcaaatta atgcgcggga agcagatgga agggaagtcg
     7801 atagggaatg tgaaaaattg ggcgaggtaa ggtaggtatc tattccctat aagcaaaata
     7861 ccgcacatct gcgcgtcgca atctaaaatc gtcaatcgaa cattgagagc tgggcccagc
     7921 agaattcgaa cccccgaccg tcattgaata taaagaggca ctacgggcag gaaaatgcga
     7981 tgaagaaact tacttttggt gtgacggacc ggctcaaaca attacagcaa ttgccttagt
     8041 atagaaatga atttatgatg aaagagagaa gctatccaga gtttaggact gcttgtcaaa
     8101 aaatagaaat agctcctcat aaagctaaca tgcctttggc cacacgtgct gaaaaatgca
     8161 acgacatagg atatcgtcag tgagaataag ctctacttct ctctcgggtt tccccgtagt
     8221 tagtcatcac gagtgacgct tcttttctca gatcgttgga ttctctggtt gtttcaacaa
     8281 catcaggtgt ctgccgaggg tgactcaacg gatttaatta tttgaacgtc atcctttcaa
     8341 gaaaaggcta aagcagcttt atgcgtatct tagccaatta cttgagattt tgaagaaagt
     8401 ccagtattcg aaccgtctca tgtgttttac caccccaatc agaacctcca tacgacctta
     8461 gctcgctcac ttcgcttagc tcgctgccgt ttaggacaga caaattgagg cccgttaaag
     8521 caatctgttg agaattttca gggttgatgg cggcggcgtc ccatgtgttc tggatctcgg
     8581 tagtgttgcc ggtctccgga ttgtagtaga caatgttgtc gtcagtcctg caggcgaaca
     8641 atcttttttg atggtcggct tccactcgct ggcaaacttg ttcggtttgg ttgaccttac
     8701 cggtggattc aacgacggag agtaaatttc cttcaacagt aagggcgacg taaatacctt
     8761 gcatcgttac gaccgaaatt ggattgatgc cgaatgcacc tttgcccatc attcgatcgg
     8821 tttcaatgtt gatctccata agctggccgt ctgcctcgcg gatagcaaag agcagggggg
     8881 agctttcttt caatgggaat tcgggcattt tcatgccttc atattctttc agcagagttc
     8941 cgccgaaatc gtaaattctc acggagacgt taccgtcttc attccaatac tctgttgcat
     9001 acttgcaatt cgtccagtgg atagacgggt ttatgaagtc tccaatctca aactcccaca
     9061 tgttttgaac caggggaagt tctatggtcc ggccatctcg ccattcaatg taagacctat
     9121 cgtcaacttt cgttgctcga aggtatggct gtaaagggca ccaatcaggt gcagcaataa
     9181 ctttcttatg gcgaaccagt tgggtcggag gtgttttgac aattcggcat ttgttgctga
     9241 agtcacctgt gtcagtatcg tactgatgct catcacggct tgggaagtag aaagtgactg
     9301 tgtaatagat cgtttctccc cagttttcga tgtcaggcac atttaaggtt tcgctatccc
     9361 aaacatcaag ccaaaagtta ggtgtcgtgt aggtatgacc ctgaccatcg gtgaacgttg
     9421 cgacaccata aggatccgga ccgttggctc cctctatgtt gcatgcttcc tcatgctcat
     9481 tgacccaggc caatgcctca agagggttat ttagggtttc gggcatatgc gggatgaaga
     9541 aggtagcggg atcgactggg ccgttgctgt tttcagcatt gtcgggatca ttcttattct
     9601 tacacttctt ctttctttcg ccattcttct ttttgcactt attcttcttg tccttgtcgt
     9661 cgttattgcc actgtcggct gcctggacgg ttgtaacgcc cagattggga gctggcatga
     9721 actcgggcag aacgaagaaa attaaagcca gccctatggc tagcttgatt aatagcttgt
     9781 tcataatatt ttccttttga gcgaacttcg tggaatttga tttattaaga attctaatgt
     9841 ccacattcta ttttcacatc cgcttcaata tttctcgtat ccataaaaat aaaagccttt
     9901 agtttagcgc ccggtcctcg cgttctttga tgcctttcag ttgttccgaa agtgcttaat
     9961 cccattgccg gctttgatca ctatttccag caggtgaata aagctagcac gacccagccg
    10021 ccaagaaacg cttgaagcat gtcaagtctc atgtcgttta gaacaagagt attttgtgga
    10081 agtgtaggtg cctatccctc tgcgatcact tctattttgc cacaggcaga tttattgaga
    10141 gtaaataaag atctgattat cgaatgctta aggcaaaacg aaacacaact tgatttgcgg
    10201 caaaggtaga gaggaatttt ctcgtgaacc ttctggcaat caagtgagca ccaatgtatt
    10261 cggttttttt gtcagtcagt gcggtttact tattggctac cttcgtctag aaaatctagc
    10321 agaagtagaa aagttcacaa aaggactatg aaagcaacga gaagatttct aactacaggg
    10381 taaatctaca ggtcaag
//
LOCUS       DASTJP010000219         3255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1064146, whole genome shotgun sequence.
ACCESSION   DASTJP010000219 DASTJP010000000
VERSION     DASTJP010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064146"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2612)
                     /locus_tag="VFQ23_06770"
     CDS             complement(<1..2612)
                     /locus_tag="VFQ23_06770"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906323.1"
                     /translation="MGNYSTNLPLQLTSFVGREREIREVKQLLCAGKTRFLTLTGAGG
                     CGKTRFALQVASDLLEQFPDGIWLIELASLSNPTLVPQAIASIFDLRESSDCSLTDGL
                     RNYFRSKHLLLVLDNCEHMIEACAHIVGTLLRSCPHLYILATSRESFDILGETTWHLS
                     PLTLPDLVEMSKPGADPISTLTQSEAARLFVDRALAALPSFRLTERNVMAVAQICLQL
                     DGLPLAVELAAAWIKLLSVEQIAARLKDCFLLLTTGERTALLHHKTLRATMDWSYGLL
                     TNKEQKLFRRLAIFDGGWTLPAAEKICSDEEIDARQVMDLLLRLVGKSLICVGERDGK
                     VRYHLLEMVRQYAQEKLLESGEREMIQFQHRAFFLNLLGDARSGLRSVEQTSWLEQIE
                     SELDNFRATLEWSLRQAVQTDEEVGLALIVELSEFWLRRNYWKEGREWIERALKAFSP
                     EEATPTRIKALHFAFQFTTNMGDINAARLYLQESGKLVFALQNKKDIAELMLNRAILA
                     RLEHRLDEAQTFAEESLQLFTELDDKEGVVSVLSILGKIEQSQGNEMAARAHFDESLR
                     LCQEVGHLEMFGRSLDLSASAAFRAGDFVKARSLFKELLTLGEQKEDKSLMRHAFLHL
                     GEVARSVGDNEQARTHYEASLVLARELSAKLYIGFALGGLGYVAIHEGKPYLARQYLK
                     ECLAIYQEENLEWGIAFAVEGLAGLAAMQGKPVSAARLLGAVDSHHALDGWAATPADR
                     IEHACTLSTVRSRLDPKAIDAAWISGQKMPLEQVAEYALAETAVLDVTPSLPETLITQ
                     VKAEPALRIFAFALPRVSRGEREITSSDWKYTKVREMFFYLLCHASRTKEQIGLVLWP
                     DASQAQLRSNFRVA"
     gene            complement(2935..>3255)
                     /locus_tag="VFQ23_06775"
     CDS             complement(2935..>3255)
                     /locus_tag="VFQ23_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9906324.1"
                     /translation="RLQSPEQRARFDDTTKCIMCAACTTSCPSYWGSDDYLGPAALVA
                     AHRFIYDSRDEAAAERMHIISETSGLARCHTIFNCTMACPRDIQITKAIGELKMTSLT
                     GRLE"
BASE COUNT          792 a          911 c          781 g          771 t
ORIGIN      
        1 atagctacgc ggaagttgct ccgcagttgt gcctgagaag cgtcgggcca cagtaccaga
       61 ccgatttgct ctttggtacg cgaagcatga cacagaaggt aaaagaacat ttcacgtacc
      121 ttggtatatt tccaatcgga ggaagtgatt tcccgttcgc cgcgactgac acgcgggaga
      181 gcgaaggcaa agatgcgaag tgcaggttcg gccttcactt gagttatcaa ggtttctggt
      241 agtgagggtg tgacgtccaa cactgctgtt tcggcaagcg cgtattccgc aacctgctca
      301 agtggcatct tttgccctga tatccatgcc gcgtcaatcg cttttgggtc gagccgcgaa
      361 cgcaccgtgc tgagagtaca tgcatgctca atgcgatcag caggcgttgc cgcccatccg
      421 tccagggcgt ggtgtgagtc cactgcacca aggaggcgtg cagcagatac tggtttaccc
      481 tgcatcgctg cgagcccggc caatccctcc accgcaaatg caattcccca ttccaaattc
      541 tcttcctgat agattgccag acattccttg aggtattgcc gcgcaagata aggcttcccc
      601 tcatgtatgg cgacataccc cagacctccc aaggcgaaac caatatacaa cttggctgat
      661 aactcgcgcg cgagaaccag gctcgcctca tagtgcgtcc ttgcctgttc gttatctccg
      721 acagagcgcg ccacctctcc taaatggagg aacgcgtgcc gcatcaatga cttgtcctct
      781 ttctgttcgc ccaatgtcag gagctcttta aacagcgagc gcgccttcac aaagtctcct
      841 gctcgaaacg ctgcagacgc tgacagatct aatgatctgc caaacatttc caggtgtccg
      901 acttcctggc agagacgtaa gctctcatca aaatgggctc gcgcggccat ttcgttccct
      961 tgactctgtt ctatttttcc caatatgctc aataccgaga caacaccttc tttatcatcg
     1021 agctcggtga agagttgcag gctctcttct gcaaaagtct gtgcttcgtc tagacggtgt
     1081 tccaatcttg ccaggatggc gcgattaagc atcagttcag caatatcctt tttattctgc
     1141 agtgcaaaga cgagtttgcc actctcctga agatacagcc ttgcggcatt aatgtcaccc
     1201 atgttcgttg taaattgaaa agcgaagtgg agagccttaa tacgcgttgg tgtcgcctcc
     1261 tccggggaga atgcttttaa ggcacgctcg atccactcac gcccctcctt ccaataattc
     1321 cgcctcaacc agaattcgga aagctcaacg atcaacgcca atccgacttc ttcatccgtc
     1381 tgtacggctt gacgcaatga ccattccaat gtcgcgcgga agttatcaag ctctgactct
     1441 atctgttcaa gccaggaggt ctgctccacg ctacgcaatc ccgatctcgc atcacccagc
     1501 aagttcaaaa agaatgcgcg atgttgaaat tggatcattt ctctctcccc cgattccaac
     1561 aatttctcct gcgcatactg acggaccatt tcaaggaggt gatagcgtac tttaccgtct
     1621 cgttcgccta cacaaatcag tgacttacct accagacgta ggagcagatc cattacctgg
     1681 cgcgcatcga tttcctcgtc gctgcaaatc ttctccgcag ccggcagtgt ccaaccacca
     1741 tcaaagattg caaggcggcg aaacagtttc tgctctttgt tcgttagcag tccataactc
     1801 caatccattg tcgcgcgcaa ggtcttatgg tgcagcagag ccgtgcgttc acccgtggta
     1861 agcagaagaa aacaatcctt caggcgcgca gcaatctgtt cgacggatag cagtttgatc
     1921 caggcggcgg ccaattccac cgcgagtggc aagccatcga gttgcaggca gatctgtgcg
     1981 actgccataa cgttccgttc cgtcaaacga aagcttggca gcgcggcgag tgctcgatcc
     2041 acaaacaacc gggcagcttc agactgcgta agggtcgaga tcggatctgc tcctggtttg
     2101 gacatttcga caaggtcagg caaagtcagt ggagataaat gccatgtggt ttcgcccaag
     2161 atatcgaatg attcacgact cgttgccagg atatacaaat gtgggcagga gcgcagcaag
     2221 gtcccaacta tgtgcgcgca ggcctcgatc atgtgctcac agttgtccag gacaagcaac
     2281 aggtgtttcg aacgaaagta gttcctgagt ccatcagtca gggaacaatc agaggattcg
     2341 cgcagatcga agattgaagc aatggcctgt gggacgagcg ttgggttaga cagagaagct
     2401 agctcgataa gccagatgcc atccgggaat tgctccagca gatcgctagc tacctgcagg
     2461 gcgaagcgcg ttttgccaca accgcctgca cctgtgagag tcaagaagcg cgtctttcct
     2521 gcgcacaaca attgcttgac ctccctgatc tcgcgttcgc gtccaacgaa actagtcaac
     2581 tgtaatggca ggttagttga ataatttccc attgttgacc tttgcttcca aagcgactgc
     2641 catcacgcag caatgctcca tgctcagaat tgctgggaac cacacccata atgcggcagg
     2701 ataaattatg ttctcacgaa acttctaaat gagatgtttt gttattcatc tccctctcac
     2761 catatccaac gtcaatatag cactttcagt aggtgaattc aagtgtttgt atgttaaaac
     2821 ccgggaaggg aacatacatt acttcgcaag tttaaaaaaa caagccgcat ctcaccgaca
     2881 cggcctgtca atctccaatt ctctaagctc tactcaccaa taccctcttg ccaattactc
     2941 cagcctgccc gtcagcgaag tcatcttcaa ctcaccgatc gcctttgtga tctgaatatc
     3001 ccgcgggcat gccatcgtgc aattgaagat cgtgtgacag cgcgccaagc cggatgtttc
     3061 actgatgata tgcatacgct cggcggcggc ttcgtcacgg ctatcgtaaa taaaacgatg
     3121 cgcagccacc agcgcggcgg gaccaaggta atcgtcactg ccccagtacg aaggacagga
     3181 tgtggtgcaa gccgcgcaca taatgcactt ggtggtatcg tcaaagcggg cgcgctgctc
     3241 aggcgattgc agccg
//
LOCUS       DASTJP010000220         5021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_268461, whole genome shotgun sequence.
ACCESSION   DASTJP010000220 DASTJP010000000
VERSION     DASTJP010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268461"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFQ23_06780"
     CDS             <1..194
                     /locus_tag="VFQ23_06780"
                     /inference="COORDINATES: protein motif:HMM:NF021429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HET9906325.1"
                     /translation="HGITLETIITKLVEDYGWAELGKRIPIKCFNENPSIKSSLKFLR
                     RTQWARKKVEDLYLKSAAK"
     gene            complement(339..1613)
                     /locus_tag="VFQ23_06785"
     CDS             complement(339..1613)
                     /locus_tag="VFQ23_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906326.1"
                     /translation="MSYQYAGLEQVHAPGLSVAEAAERLCRFAYAEERLMRLFAARIV
                     TIPERDIKVLMGRLQYEASLHVSNWRARVVELRTPKSKLERVPDVALERFFDEADELK
                     ETYSFLAVVTKLIKPALRHAYQKYLRETNGVADYGSVRFIQQHLVDEEEHLRLLTLAL
                     DDLTPAAKPEELESAKMSEVRLATYLAAAGGIDGTQPRIDMPDETRRAKKSKRIPREL
                     TRDETLPRVWDFVKPPLESTDAHLNYMMSIRLSEVNVAEGLAIVLCEQEDMPWSFYAD
                     ISRHCWDEMRHSIFGEVAIEATYGDRAALALRDYEGIYALEAPPLEQYTVLGLEVEGQ
                     NMRYPPGKRQEWEFARDMAKHPLMTTLQDFDWADEVLHVNIARRQLDTWFEGGLAAIR
                     DFAKAGKANRTAVKGRQSPTPLPSPQASKQPE"
     gene            complement(1610..2521)
                     /locus_tag="VFQ23_06790"
     CDS             complement(1610..2521)
                     /locus_tag="VFQ23_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET9906327.1"
                     /translation="MSDVLCFGNLQLDVLCRTVTELPPPGSLQKIDTVDFALSGNGGS
                     LAMALARLGVSVDLAGYSGADVIGDQFRTMLMTEGVGIDKLTRHPTAGTGTSIVTVAP
                     TGERSIFYVNGANDEVNLESVPDDWLHGLRILAVTSVFVLPHFTGEAIAQLFQRAHAA
                     GARTVLNICWDGEARGLNFLAPALAHSDYFVLNFDEGCQLTGFETPNEIIGRLMDFTS
                     GTIILTLGAEGCCFRTESGIERVPAIPVNATDTTGAGDSFVAGFIAGLIRGRSLSECV
                     QLGCEIAAYAVTGTGAYVRIPRYTEEV"
     gene            2802..3218
                     /locus_tag="VFQ23_06795"
     CDS             2802..3218
                     /locus_tag="VFQ23_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906328.1"
                     /translation="MSSEMFFLVALAAGSVLFVILLIIVAILRGIRKNASEKRNSAAS
                     SAPKGDGVHVVKRGRDGEVLYIENGNTGRFYWEFLGGDAIIGVTFPKQDQWDTAIPWA
                     AGRQDEIMQKVAKEVVRKEARGHHVRWADGSFEILK"
     gene            3385..4446
                     /locus_tag="VFQ23_06800"
     CDS             3385..4446
                     /locus_tag="VFQ23_06800"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HET9906329.1"
                     /translation="MAQKGYFILTDISGYTEFLTRSELDHAQDALQNLFDIQLAHIKY
                     PFVISGFRGDAIFIYVPEANICEPQTILEALENLFFAFATARRQMIYNTTCPCRACQN
                     IARLDLKMVIHYGEYALQKLGDREELLGADVIVPHRMLKNNVIEKTGIESYALFSDAA
                     ADAAQLSQLAYPLIGHSETYEHIGEVKMHVLDLRKVWEREQEKTRFVVTAEEAWLNFE
                     WEVPDAPLAATWENLTNPRRELQWSGYDLVERTDALGGRLRPESTYHCAHGDLHIFNK
                     ILDWKPFEYISIEQNISLGIQLVQTRRVQETANGTKIIFYVRQPEAPVSDELRQVVID
                     AYNQAAARLIKHLEVEHHE"
     gene            4537..>5021
                     /locus_tag="VFQ23_06805"
     CDS             4537..>5021
                     /locus_tag="VFQ23_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9906330.1"
                     /translation="MSSNPFQVRVQPSLDPPGLRLRAQATTASDTLAFESPGTVLTVL
                     ETEASARPKLGVNGQWLRVRDANSREGYVAAWYVEAATGITTTTSPQTSKSPPTAAPS
                     AQALVDAINAERLKKNVQTLKVHSILMNSAQNHAEYMASGGGITHYSADGSRPFQRHL
                     A"
BASE COUNT         1257 a         1318 c         1286 g         1160 t
ORIGIN      
        1 tgcacgggat tacgcttgaa acgataataa caaaactcgt ggaggattat ggctgggctg
       61 aactgggcaa gcgtatcccg atcaaatgtt ttaacgaaaa tcccagtatc aaatccagct
      121 tgaagttttt acgtcgaact cagtgggcta gaaagaaagt ggaagatttg tatttgaagt
      181 ctgcagcaaa atgaaccgca tgacgtgaaa gcattacaag agagtcagtc agagtggcaa
      241 gcgtcaacgc gctccgggag tgtataactg ctccctcacc ctaccctctc cctaagggag
      301 agtgcataca cgcagcaaag caggacaata ataaggcttt attctggctg tttgcttgct
      361 tgaggagatg gcagtggtgt gggagactgc cgccctttga cagccgtccg gttggctttg
      421 cctgctttgg caaagtcgcg gattgcagcc aggccgcctt cgaaccatgt atccagttgg
      481 cggcgcgcaa tattgacgtg taacacctca tctgcccaat caaaatcctg taaggtggtc
      541 atcagtgggt gcttggccat atcgcgtgca aactcccatt cctgtcgctt gccgggcggg
      601 tagcgcatat tctgaccttc cacttcaaga cctaatacgg tgtactgctc gagtggcggt
      661 gcctccagtg cgtagatgcc ttcatagtcg cgcaacgcca gtgccgcgcg gtctccgtat
      721 gtcgcttcaa tcgcgacttc gccgaagatc gagtggcgca tctcatccca acaatgacga
      781 gaaatgtccg catagaaact ccagggcatg tcttcctgtt cacacaggac gattgccagc
      841 ccctccgcca cattcacctc gctcaggcga atgctcatca tgtaattgag atgagcgtcc
      901 gttgattcca ggggtggctt cacaaaatcc cagacgcgcg gtagggtctc atcacgcgtc
      961 agttcgcgcg ggatccgttt cgatttcttt gctctgcgcg tctcatcagg catgtcaatc
     1021 cgcggctgcg ttccgtcgat gccgccagcc gcagccaggt aagtagccaa cctcacttcg
     1081 gacatttttg cagattcaag ttcttccggt ttggcggctg gcgtcaaatc atccagcgcg
     1141 agagtcagta agcgcaagtg ttcttcctca tctaccaaat gctgttggat aaaccggaca
     1201 ctgccatagt cggcgacgcc gttcgtttct cgcaggtatt tttgatacgc atggcgcagc
     1261 gctggcttga tgagttttgt gacaactgcc agaaatgaat atgtttcctt cagttcatcc
     1321 gcctcatcga agaatcgttc gagggcaaca tccggcacgc gttccagttt gcttttcggc
     1381 gtacgcagtt cgaccacgcg ggcgcgccaa ttcgagacgt gcaagctcgc ttcatattgg
     1441 agccgaccca tgaggacttt gatatctcgt tccgggatcg tgacgatgcg ggcggcgaaa
     1501 agacgcatca gtcgctcctc cgcgtaggca aagcgacaga gtcgctcggc ggcctcggca
     1561 acactgaggc cgggtgcgtg aacctgttcc agacccgcgt attgataact catacttcct
     1621 ccgtataacg tggaatgcga acataagcgc ccgtgccagt gacggcatag gcagcaattt
     1681 cacaacccag ctgaacacac tcgctgagtg aacgtccgcg aatgagacct gcaatgaaac
     1741 cggcgacgaa gctatcgccc gcgccggtcg tatctgtcgc attgaccggg atcgccggaa
     1801 cgcgctcgat tccagattct gttcgaaagc agcaaccttc cgccccaagc gtgagaatga
     1861 tcgtaccgga cgtaaagtcc atcagacgcc cgatgatctc gtttggcgtt tcaaaaccgg
     1921 tcagttggca gccctcatcg aaattgagca cgaagtaatc actgtgcgcc agtgctgggg
     1981 ctaagaagtt cagaccgcgt gcctcgccat cccagcaaat gttcaatacg gttcgagcgc
     2041 ccgcagcgtg cgcacgttga aaaagttgag caattgcctc gccggtaaaa tgaggcaaca
     2101 cgaagaccga agtcactgca agaatgcgta agccatgcag ccaatcatca gggacactct
     2161 ccaaattaac ttcatcattc gcgccgttta catagaaaat acttctttcc cccgtaggag
     2221 cgaccgtaac gatcgaggtg cccgttccag ctgtagggtg acgagtcagt ttatcgatgc
     2281 cgacgccctc agtcataagc atcgtgcgga actgatcccc gatgacatcc gcgccgctat
     2341 aaccggcaag gtcaaccgag acgccgagcc gcgccaaagc catagccagg ctgccaccat
     2401 tgccgctcag cgcgaagtcc actgtatcga tcttctgcag gctcccgggt ggcggcagtt
     2461 cagtaacggt ccgacataat acatccagct gtaaatttcc gaaacacaaa acatcagaca
     2521 taataatttg cctctgcgtg agattcagat tctagcatat ctacacagat ctaaattgtg
     2581 ggagtgccta cattttaaac aggcacccgg taagctttga gcgtggataa tgcagttcca
     2641 aagttaactc attaatcagg cgagccatag aaaccatttg ctgttatatt aaacatcgtc
     2701 gtcaccatct aataaccttg gcgcgctcac ctgcaggatt taaaattgga tcctgttgac
     2761 cgataagaag aacacataaa acacatcaca gaggagatat tatgtcatcg gagatgttct
     2821 ttttagtagc tctcgcggct ggtagtgtgt tgtttgttat cctgctgatc atcgtggcta
     2881 tcctccgcgg gattcggaag aatgcttcgg aaaaacgtaa ttctgcagcc tcctcggccc
     2941 caaaaggtga cggtgtccac gtcgtcaaac gcgggcgcga tggtgaagtc ctgtatatcg
     3001 agaatggcaa tactggtcga ttctattggg aattcctggg cggtgatgcc atcatcggtg
     3061 ttacttttcc caaacaggac cagtgggaca ctgccatacc ctgggcagcg ggccggcagg
     3121 atgagatcat gcagaaagtt gctaaagaag ttgtgcgcaa ggaagcgcga gggcaccatg
     3181 tccgctgggc cgatggtagc tttgaaatcc tgaagtaaat caagctggca cgtgacctga
     3241 atcagttgaa gatttgaggt cctatcctca tcactatcga gactctccct gtcgggtccg
     3301 tgctgcgatc gtacgtgaag gtttgtgatt tgaaaaccgt acagtaataa gctatatgag
     3361 tcttgtcttt tgcttggagc actgatggcg caaaagggct atttcatcct caccgatatc
     3421 tcaggctaca ccgaatttct aacgcgctct gaactggatc atgcgcagga cgcgcttcag
     3481 aatttgttcg atatccagct tgcccatatt aaatacccgt tcgtgatctc tggctttcga
     3541 ggggatgcaa tctttatata cgtacctgaa gccaatattt gcgagccaca aaccatcctt
     3601 gaggcattgg aaaatctatt ttttgccttt gccacagcac gacgacagat gatctacaac
     3661 actacctgcc cctgtcgcgc ctgccaaaac atcgcccggc tcgacctaaa aatggtgatc
     3721 cactatgggg aatatgctct tcagaaatta ggcgaccgtg aagagttgct gggtgccgat
     3781 gtcattgtcc cgcaccgcat gttgaaaaat aatgtgattg agaagacagg cattgagagt
     3841 tacgccttgt tcagcgatgc cgcggcggat gctgcacaac tatcacagtt ggcataccca
     3901 ctcatagggc attccgaaac gtatgaacat atcggggaag ttaagatgca tgtccttgac
     3961 ctgcgtaaag tctgggaacg tgaacaggaa aagacccgct tcgttgtgac ggcggaggaa
     4021 gcctggctga attttgagtg ggaagttccg gatgctccgc tggctgcaac gtgggaaaac
     4081 ctgaccaacc cgaggcgtga gctgcaatgg tctggctatg atctggtcga acgaaccgac
     4141 gcgctgggcg ggcgtcttcg acctgaatca acttatcact gcgcgcatgg cgatctgcac
     4201 attttcaaca agatactgga ttggaaacca tttgaatata tttcgatcga gcaaaatatc
     4261 agcctcggaa ttcagttggt gcagacccgc cgggttcagg agaccgccaa tggtaccaag
     4321 attatctttt atgtgcgcca accagaggct cccgtctcgg atgaactgag acaagttgta
     4381 attgatgcgt acaaccaggc tgcggcgaga ttaataaaac acctggaagt tgaacaccac
     4441 gaatagaaat gacattcaag cgagcgttgt gagctcgaaa attttataaa gactggaagc
     4501 tatctgtagg tacttgccat tgcaaaggag ttgatcatgt catccaatcc atttcaagtt
     4561 cgcgtgcaac cgtctcttga cccgcctggc ttacggctac gtgcgcaagc gacaaccgca
     4621 tcagacacat tagctttcga atcaccagga accgtgctta ccgtcctgga aacagaagct
     4681 tccgcaagac cgaaacttgg ggtcaacggt caatggctac gggtgcgaga cgcgaattca
     4741 cgcgaaggat acgtcgctgc gtggtatgta gaagcggcaa caggtataac tacaaccact
     4801 tcaccacaaa catcaaagtc acccccaaca gccgcgccaa gcgctcaagc attggtggat
     4861 gcgatcaacg ctgagcgact gaagaaaaat gttcaaactc taaaggttca ctcgattttg
     4921 atgaacagtg cgcagaacca tgccgagtac atggcatccg gcggcgggat cacccactac
     4981 agcgcggacg ggtcgcgtcc gttccagcgc catcttgcgt t
//
LOCUS       DASTJP010000221         5927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_322369, whole genome shotgun sequence.
ACCESSION   DASTJP010000221 DASTJP010000000
VERSION     DASTJP010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322369"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFQ23_06810"
     CDS             complement(<1..298)
                     /locus_tag="VFQ23_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906331.1"
                     /translation="MRRDTIQALLVLILILVAVVLPVISAGYSDLKKASISTTYVEAA
                     QHYQSAAERLPWRADLYELSGHAYYHAQEYTNADAAYQKAFQREALSSEGWVAWG"
     gene            418..2784
                     /locus_tag="VFQ23_06815"
     CDS             418..2784
                     /locus_tag="VFQ23_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9906332.1"
                     /translation="MGATLDQFFSVLTQPEGSLIYHLILVFSIAGALQAAFVHWRSSE
                     FPQARRTMVGLGILLVAQISLFIIGGIGIQGFINLPLFLPPLDRAITVFSLLWMIWLW
                     AFPEPSRPADAATWLLSLLVGAALGLSLVASSAATSISPYNQTTYEVFWQIASIVLSV
                     LGLFILILRRPNGWGYGIAVFLLCFAGHLLHLLFGRIGGDFPGAVRLAHMAAFPILMT
                     LPQRFPAPTSALTTVKQQQDVPTGGERRRYSTDPKTFHALLALAGESSADKVSEAITR
                     AIAQTMLADLCFLIYLTDNKNQMQIASGYDLIREENLEGGSMSKTVIPMLTNALQRGR
                     PLRLPSSSTSADIKGLSDLLGLTNPGHLLSVPIITPEKDPIGGVLLLSPYSNRLWSAE
                     DQAFLTNIAISLVPIIQRGQKMTSLEQKGEQTKQALDVAQSRISDLERRNNDLIKQMD
                     AVRADAQEGLAQAENLAALTKMQEEAQIAIERLKQENEDLRAAKVVKGSANASSGNSI
                     QIENELRITLEEVARLQNQLAEANMRVIEAEKSTSTAHSTEQAEVVASISQELRQPMS
                     SIVGYTDLLLGESVGILGALQRKFVERIKASTERIGALIDDMIQVTTLESGLNELKPE
                     PVDLNVIIDNAMSYTSSQVREKNISMHLDLPKNVAPIYADREALQQILIHLLQNAGAA
                     SPLEGTVRLKVQTKAEDGKEYILIQVSDTGGGIPSQDLQRVFTRLYRADNVLIQGVGD
                     TGVGLSIAKTLTEAQKGRIWVESEDGVGSTFSVLLPIAKNAVMKTAEK"
     gene            2786..3595
                     /locus_tag="VFQ23_06820"
     CDS             2786..3595
                     /locus_tag="VFQ23_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9906333.1"
                     /translation="MQSLRQVSIGIIIALASVVLVIGGIFLSLAETFSPLSTPTPIPP
                     TLQLTFPTITPSLGIAQSETPTATASLAQVESQTASVTLAPIATCSPAPASWIRITLT
                     VGDTIYSVAQRYLTTTEDLSAANCLTSLDLPVGYVLLVPPVQATVTAVPCGPFAGWVR
                     AYIVQPGDNLFRIGISYNVTVTQLQRANCMGSSTTIFVGQRLWVPNVPTRTPGITVIP
                     NSPTASNTSQPVPTTEVPTLEPPTATSTQIPTTEVPPTPTASNTSPPPQSP"
     gene            3592..5079
                     /locus_tag="VFQ23_06825"
     CDS             3592..5079
                     /locus_tag="VFQ23_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9906334.1"
                     /translation="MRSVLWIVIIASLLLAGCGTSASANAQPPLPFAFVTVAPNASAT
                     PTPFQPLGVSPTGSSFSENSTQASPTPDPATQTATSPAPTQTEVPTIDANMLINTVAP
                     LPTIDASGSQVLNNGQETVNFLLIGSDRRSTAGSTRTDTMVIAILRPNEGQVSLISIP
                     RDLWVSIPGGYENNRINTAYQLGISSGYPGGGPGLLKETILYNLGIRIDHTAMVDFSG
                     FSQIVDTLGGVDVPVSCAYTDWRLIDPSFDPFNENNWHLYTTGPGMIHMDGDLALWYA
                     RSRQRSSDFDRGRRQQEVLRALFTQALQGGTVARIPELYNDLQSTVETDLGLADLLQL
                     AIYAPKMTSADIRSYYIRPPLVSSWITDGGAYVLLPNEFELQQMLTEALSPSTKTVEK
                     QTIVIDVMNGTSVAGYEALATSRLNYAGYETHIVPSDRTDYAYSVLIDKTPLQDISMS
                     NPILDVMGMLQGNIISSPDANSTAHYLLILGYDYEPCFSPENLSQ"
     gene            5119..>5927
                     /locus_tag="VFQ23_06830"
     CDS             5119..>5927
                     /locus_tag="VFQ23_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906335.1"
                     /translation="MRSKINPILFVILSLVFASLACQAFSGGTQPASTSEPQQTEPPQ
                     QPVSTATAAQAPVEPATIESQGVGIACVGSGTGLNCINENGWQLYTDENSDLPNNYLY
                     AGAVCPDGKLAIAHISGVALFDGTTFSLIPETDAFSTPDGIACTADGEVWVAHFQGVS
                     QYANGQWTTHGADQLASGESANELIYDVEVAPDGKVWVVTSRSVAMFENGAWTVFQEG
                     QGFNGSRFFNALALDASGRPWAAHSGGVDVFENEAWRSIEKSDFTSPESLAV"
BASE COUNT         1460 a         1602 c         1469 g         1396 t
ORIGIN      
        1 ctccccatgc cacccaacct tctgacgata gagcctcgcg ctgaaaggct ttctgataag
       61 ctgcatctgc atttgtatac tcctgcgcgt ggtaataagc gtgaccagag agttcgtaaa
      121 ggtcagcgcg ccagggaagg cgttctgctg cagactgata gtgctgcgcc gcttcaacat
      181 acgtggtgga aatagatgct tttttcaagt cggaatatcc cgcgctgatc acaggcagga
      241 caacagcaac caggatcaat ataaggacca agagtgcctg gatcgtatcg cgacgcatag
      301 gctgattata aaggttgact caaggcgagc ttaagcggca ttaatttacg atcttgtaac
      361 taatccaatc aaaaatttcg cgccagattt tggtaactgg aatataatca cttatgtatg
      421 ggcgcaactc ttgaccagtt tttttccgtt ctcacacaac ctgaaggcag cctgatctat
      481 cacctcatcc tcgtcttctc catagcgggc gcattacagg cggcttttgt tcattggcgg
      541 tcaagcgaat tcccacaggc gcgacggacg atggttggtc ttgggatact cctggtcgca
      601 cagatcagtt tattcatcat cggtgggatc ggcatccagg gctttatcaa tttgccgctt
      661 ttcctgcctc cgctcgatcg tgcaatcacg gttttttcgc tactatggat gatctggcta
      721 tgggcattcc ctgagcccag ccgtccggcg gatgccgcca cctggttgct aagtctgctg
      781 gtgggtgccg cacttgggct aagtcttgtc gcgtcttctg ctgcgacttc catctccccg
      841 tataaccaga caacctacga ggtattttgg cagatcgcta gcattgtgct ttctgtattg
      901 gggcttttca tcttgatcct tcgccgcccc aacggatggg gttatggaat tgccgtattt
      961 ctactgtgct tcgccggtca tcttcttcat ctgttattcg ggcgtatcgg cggtgacttc
     1021 cccggcgcgg tgcggctcgc tcacatggcg gcgtttccga tcctgatgac gttgcctcaa
     1081 cgctttcccg cccccacaag cgcgctcaca acggtgaaac agcagcagga cgtgccaaca
     1141 ggcggtgaac gccgtcgtta tagtacagat cccaagacgt tccacgcact gctggcatta
     1201 gctggtgaat ccagtgcaga caaagtcagc gaagccatca ctcgtgcgat cgcccaaacc
     1261 atgctggcag acctgtgctt tctgatctat ctcactgata acaaaaatca gatgcagatc
     1321 gccagcggct acgacctcat ccgtgaagag aaccttgaag gtgggagcat gagcaagacc
     1381 gtgatcccca tgctcaccaa tgcacttcag cgtggacgac cactgagact tccctccagt
     1441 agtacatcag cagatatcaa aggcttgagc gatttgctgg gtctcactaa ccccggtcat
     1501 ttgttaagtg ttcctatcat cacccccgag aaagacccga tcggtggagt gcttttgcta
     1561 tctccgtatt cgaatcggct ttggagcgct gaggatcagg catttctcac gaatatcgcc
     1621 atctctctcg tccccatcat tcaacgcggc cagaaaatga cctcgcttga acagaaaggc
     1681 gaacagacca agcaggcatt ggatgtcgca caatcccgta tttcagacct cgaacggcgt
     1741 aacaacgatc tgataaaaca gatggatgca gtcagagctg atgcccagga agggcttgcc
     1801 caagccgaga accttgccgc cttgaccaaa atgcaggaag aagcgcaaat cgctatcgaa
     1861 cgactcaaac aggaaaatga agatttacgc gcggcaaagg tagtcaaagg aagtgcgaat
     1921 gcatccagcg gaaactcaat ccaaattgag aatgaattgc gcatcacgct ggaagaagta
     1981 gcgcggctgc aaaatcagct tgctgaagca aatatgcgcg tcatcgaggc cgaaaaaagt
     2041 acatccactg cgcattcgac cgagcaggca gaagtggtgg cttccatttc acaggagtta
     2101 cgccagccga tgtcctccat tgttggatac acggatttgc ttttgggtga atcagtcggt
     2161 attctgggcg cgctgcaacg caaattcgtt gagcggatca aggcctccac agagcgcatc
     2221 ggagcgctca ttgatgatat gattcaggtg acaactcttg aaagcggact caatgaactt
     2281 aaacctgagc ccgtcgactt gaatgtgatc attgataatg cgatgtccta tacaagcagt
     2341 caggtacgtg aaaagaatat ctccatgcat ctggacctgc cgaagaatgt ggcgcccatc
     2401 tatgcagacc gtgaagcctt gcaacagatt ttgatccatc tgcttcaaaa cgcgggagcc
     2461 gcttcaccgt tggaaggaac ggtccgctta aaggtgcaga cgaaagcaga agatggcaag
     2521 gaatacattc tcatccaggt cagtgacacg ggtgggggca ttccttccca ggatctacag
     2581 agggtgttca cgcgtttgta tcgtgcagat aacgtcctga ttcaaggggt aggggataca
     2641 ggagtaggtc tttccattgc aaagactctt accgaagcac aaaaggggag aatctgggtt
     2701 gagagtgaag acggagtagg gtcgacattc agcgtgcttt tgccaattgc aaaaaacgcg
     2761 gtgatgaaga ctgcggagaa ataggatgca gtcactgcgg caagtgagca ttggaatcat
     2821 tatcgcgctg gcatcggtcg ttctggtcat tggaggtatc tttctttcgc tcgccgagac
     2881 attttcacct ctctcaacac caacgccgat ccctccaacg ttacaattga catttcccac
     2941 aattacacct tcgctgggta tcgcgcaaag tgaaacaccc accgccaccg cttcactggc
     3001 acaagtcgaa tctcaaaccg cgagtgtgac gttggcgccc atcgccactt gttccccagc
     3061 acccgcaagc tggatacgaa ttactcttac agtcggcgat accatttatt cggtcgcaca
     3121 gcgttatcta acgacaacag aagacctaag cgcggcaaac tgccttacct cactggacct
     3181 accagttggg tatgttcttt tggtacctcc cgtccaggca acagtgacgg ctgtgccatg
     3241 tggtccgttt gcgggctggg tacgagccta tatcgtacaa cccggtgata acttattccg
     3301 cattggcata tcctataatg tgacagttac acaattacag cgtgcaaatt gtatgggttc
     3361 ttctacgacg atttttgtcg ggcagagact ctgggttccc aacgtaccca cgcgtacgcc
     3421 aggcatcaca gtgattccaa attcaccaac agcatccaac acctcgcagc cggttcccac
     3481 gacagaagtt ccaactctgg aaccaccaac tgcaacatct acgcaaatcc caacaactga
     3541 agttccacca acgcccacag cttcgaacac ttcaccccca ccgcaatcgc catgagatcg
     3601 gttttgtgga tcgttatcat cgcgagtctt ctcctcgctg gatgcggaac ctccgcgtct
     3661 gcaaatgcac aaccccctct gccattcgca tttgtgactg tcgcgccgaa tgcctcagca
     3721 acgccgactc cgtttcagcc acttggagtc tctcccactg gttcatcttt ttcagaaaac
     3781 tcgactcaag cttcgccaac tcctgatccc gctacccaaa cagccacctc ccctgcgccc
     3841 acacaaacgg aagtcccaac cattgatgcg aacatgctca tcaatacggt tgctccgctg
     3901 ccgacaattg acgcgtcggg cagtcaggtg ttgaacaatg gtcaagagac ggtcaacttt
     3961 ctattgatag gttccgatcg aagatccacg gctggctcca cgcgcactga cacgatggtg
     4021 atcgccattc tgcgtccgaa tgaaggacaa gtgtcactca tatcgatccc gcgcgatcta
     4081 tgggtttcaa tacccggcgg ctatgaaaac aaccgcatca acactgccta tcaattggga
     4141 atctcatccg gttatcccgg cggcggacca ggcttgttga aagaaacaat cctttacaac
     4201 cttggcattc gcattgacca tactgccatg gtggatttca gcgggttcag ccaaattgta
     4261 gataccctcg gcggcgtgga cgtacctgtt tcctgtgcct ataccgactg gcgcttgatc
     4321 gaccccagct tcgatccgtt caacgaaaac aactggcatc tctacacaac gggtccgggg
     4381 atgattcaca tggatggtga cctggcgttg tggtacgcgc gctcacgtca gaggtccagt
     4441 gatttcgacc gtggccgtcg tcagcaggaa gtgttgcgcg ctttattcac acaggcattg
     4501 cagggcggca cggtggcgcg catcccggag ctatataacg acctccaaag caccgtggaa
     4561 actgacctgg ggctggctga tcttttgcaa cttgcaatct acgcgcccaa gatgaccagt
     4621 gcagatattc gtagttatta catccgccca cctttggtca gttcgtggat caccgatggc
     4681 ggcgcgtatg ttttgctccc gaacgaattc gaactgcagc aaatgttaac cgaggcatta
     4741 tcgccctcaa caaaaacagt cgaaaagcaa acgattgtga tcgatgtaat gaacggaacc
     4801 tctgtggccg gttatgaggc gttagccacc tctcgtctta actacgccgg ttatgaaaca
     4861 catatcgtcc cctcagacag gactgattat gcctattcag tattgatcga taagacaccg
     4921 ttgcaggata tatccatgag caacccaatt ctggatgtga tgggcatgct gcagggaaat
     4981 atcatctctt cgccagacgc aaacagcacg gcgcactatt tactaatttt ggggtatgac
     5041 tacgagccat gttttagtcc cgaaaattta agccagtaaa aaacagaacg tcaaatttta
     5101 ttaactttgg agtaatgcat gagatcaaaa atcaacccca ttctgttcgt cattctgtcg
     5161 ctggtttttg caagcctggc ctgccaggca ttctctggtg gaacacaacc tgccagcaca
     5221 tccgagcctc agcagactga accgcctcaa cagccagtat ctacggcaac tgccgcacag
     5281 gctcctgtgg agcccgccac aatcgaatcg cagggggtag gaattgcatg cgttggttca
     5341 ggtacggggt taaattgcat caatgagaac gggtggcaac tctatacaga cgaaaattct
     5401 gatctgccca ataactattt atatgcaggg gcggtctgcc cggatggaaa acttgccatc
     5461 gcgcacataa gcggagtcgc tttattcgat ggcaccactt tcagcctgat ccccgaaacg
     5521 gatgcgtttt ccacaccaga cggaatcgcc tgcacagccg atggcgaagt ttgggttgca
     5581 cacttccagg gagtgagtca gtatgcgaat ggtcaatgga ctacgcatgg tgcagatcaa
     5641 ctggcaagtg gagagtctgc taatgagttg atttacgatg tggaagtcgc tcccgatggg
     5701 aaagtctggg tggtcacctc ccgaagtgta gcgatgttcg aaaatggagc ctggactgtc
     5761 ttccaggagg gacagggctt caacggcagt cgcttcttca atgctctcgc tcttgacgca
     5821 agcggacgtc cctgggcggc acatagtggc ggcgtggacg tcttcgagaa tgaagcatgg
     5881 agatctatcg agaagtctga cttcacatcc cctgaaagcc tggctgt
//
LOCUS       DASTJP010000222         4389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_342548, whole genome shotgun sequence.
ACCESSION   DASTJP010000222 DASTJP010000000
VERSION     DASTJP010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342548"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..766)
                     /locus_tag="VFQ23_06835"
     CDS             complement(308..766)
                     /locus_tag="VFQ23_06835"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HET9906336.1"
                     /translation="MAEQDPQLNAQLDFYQQMRDSIRIWLKGKGVNYQFTDYLLAAPD
                     LFHLLCKLAIDKEIPVSEKAKLAAAIVYFISPVDLLPEALSGPLGYIDDVAMAAFVLN
                     NIINKTNPEIVKRHWAGDKDILDLIQQILQVADEMVGSGLWAKIRKGFGK"
     gene            complement(951..1967)
                     /locus_tag="VFQ23_06840"
     CDS             complement(951..1967)
                     /locus_tag="VFQ23_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906337.1"
                     /translation="MDFFFKKRKPAQPQQSTISAGLPKMGDAIPDSLKAHSRFIKRVS
                     CPQCGAPKSRPSTTAYIYCDYCGSLMDYDFRLANANTNAGITNTVYHRIMMTVQLQLN
                     QAKARGDRDTVRQIYKHVFTHWLQECPTAASPRAKTDMAFRAQFIDYSAECAVIKEMD
                     PHQTTLQTQMDGLVARLQRIPTPGGAWMVAGPFWEYAALFKGQMEGTYALIRQSGAHN
                     LDPDKASETVALRMEYSSFCQAWLPHLSPADGERLLKFYGLNAEYDEVGPHDSEQRHC
                     GQCGSDIETLPGAKQVVCESCGFTIDVNSQAIPCQKCGALLSFPISTNHVVCPYCSTD
                     TRRV"
     gene            complement(2331..3302)
                     /locus_tag="VFQ23_06845"
     CDS             complement(2331..3302)
                     /locus_tag="VFQ23_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9906338.1"
                     /translation="MKAILWTKYGPPEVLHLKEVEKPAPKDNEVLIKIRAVLVSTGDC
                     EMRNLQFPLWVRVPMRIYVGFRKPRRVNILGGYLAGEVEVVGKDTKHFKIGDQVFGFS
                     GMGFGGYAEYICMPEETVIPMPADLPYEEAAPVSLGGMEALHFLRMANIQPGQKLLIN
                     GAGGSIGTYAIQLAKHYGAEVTAVDSAGKLDMMRSIGADHVIDYSKEDFSKNGQKYDV
                     ILDVVLRSSFSRIMKSLNAQGIYLMTNPTLPKMIRAAWASRFTSKKVLFQFTNRSTKD
                     LNTLKELIEAGKIKTVIDRRYSLEQVVDAHKYVETGQKKGNVVMTPG"
     gene            complement(3590..>4389)
                     /locus_tag="VFQ23_06850"
     CDS             complement(3590..>4389)
                     /locus_tag="VFQ23_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9906339.1"
                     /translation="AGTESIHNIAGLGAACQDVDKLLTKAQRVRDLKGQLITRLKEIK
                     PDCVLNSPETEAECLPNTLNITFPGVGNAGLMGMLDYRGIAVSAGSACSTGEDKPSHV
                     LKAMGLSDQAARETIRISLGSNTSTSDIGYLAQVFQDYVEGRTPYVNMITPAQLNETI
                     LFDQGTYILDVRPPDDRKKAKGLPNSHEVNPLSVEKYLDQLPRDKEIIVYCPGGGLSF
                     MISYYLKSKGFKHVSNLRGGLDAWRKRRNDLYQKYAGQNVTVLEPGK"
BASE COUNT         1011 a         1135 c         1042 g         1201 t
ORIGIN      
        1 ttcccacttg gaaagcgcga caaaatctct tggctaaatt catttcgtcc cacgagagct
       61 tcaagtgtgt aagtcatgct tcggagttac tttcctactc tgttctttgc tttcaagatt
      121 tgaagtttat ctttttctgc cagaaccgcc caacggctcg cgttacccgc ttggggtggg
      181 agggtggagt cgccgtcgaa acggaaaaag cctcaagccc gacaaatgcc caaaaaaacg
      241 tggcgcttac ccacctgtct tctgggcgca ttgttgggcg acgctggagt atattgcaaa
      301 cccatcccta tttgccaaaa cccttccgaa tctttgccca taagccactc ccaaccattt
      361 catcagcaac ttgaagaatt tgttggataa gatccaaaat atctttatct cccgcccaat
      421 ggcgcttgac aatttcgggg ttggttttat taataatgtt atttaagacg aaagcagcca
      481 ttgcaacatc atcaatatac ccaagaggac cactgagagc ttcgggtaga agatcaacag
      541 gcgaaatgaa atagactatt gctgctgcaa gttttgcttt ttcgctaact gggatttctt
      601 tgtcgatagc cagtttacaa agcaaatgga ataagtcagg cgcagcaagg agataatccg
      661 taaattggta attaacacct ttgcctttga gccaaatccg aatactatct cgcatctgct
      721 gataaaaatc aagttgagca ttcagttgtg ggtcttgttc agccatttac aaactccttt
      781 gatgcttaat gaaatcgcaa atcgcttgat ttccaggctt tcttgcgtgt tgaatttata
      841 agttgtctta agaagctgcc cagttctggc ttatacagca tacgagccgt ataacacctt
      901 ttttctatca gcaattatac attcctaaaa tcttcaggca agcgaagggt ttacaccctt
      961 cgtgtatccg tgctgcaata gggacacacc acatgattgg ttgaaattgg aaatgacagc
     1021 agcgcgccgc acttttggca cggaatcgcc tggctgttca catcaatcgt gaagccgcag
     1081 gattcgcata ctacttgctt tgcgccgggg agcgtctcaa tatctgagcc gcactgacca
     1141 caatgccgct gctcagaatc gtgaggtccc acttcatcat actcggcgtt caagccgtag
     1201 aatttcaaga gtcgttcgcc atccgccggg gagagatgcg ggagccacgc ctggcaaaag
     1261 ctcgaatatt ccatgcgcag ggcaactgtt tctgaagcct tatcggggtc caggttgtgc
     1321 gcaccagact ggcggatcag cgcgtacgtc ccttccattt gtcctttgaa taacgcggca
     1381 tattcccaaa acggaccggc caccatccac gcgccgccgg gagtgggaat gcgttgcaat
     1441 cgtgcgacca gcccgtccat ttgagtttga agcgtggtct ggtgagggtc catctccttg
     1501 atcaccgcgc actcagcaga ataatcaatg aactgtgcac ggaaggccat atcggtcttt
     1561 gcgcgcggcg aggcggccgt ggggcattcc tgaagccagt gcgtgaacac atgcttatag
     1621 atctgccgaa ccgtatcacg gtcgccgcgg gctttggctt gattaagctg taactgcacg
     1681 gtcatcatga tgcgatgata gaccgtgttg gtaatgcccg cgttggtgtt ggcgttggca
     1741 aggcggaagt cgtaatccat gagggagcca cagtaatcac aatagatgta agcggttgtc
     1801 gatggtcgag atttcggcgc gccgcactgc gggcaactca cgcgtttgat gaagcgggag
     1861 tgggctttca atgagtcggg aatcgcatct cccattttgg gaagccccgc tgagatggtt
     1921 gactgctgtg gctgtgccgg cttgcgtttc ttgaagaaga aatccatgat aaggcgctct
     1981 cctcagtgac tagggtaatg aaaactcaga tgaaaaatct ctacctacct gacactttgt
     2041 accaagatca gtcgttggtc gagtaggcgg tgttcgttcg ccgtacacct gcactggcac
     2101 acaggtgcaa gtgtcgagac caaaggtcca taagaagcca agaagtttca tgtaagttgt
     2161 ggtctcggta cgcccctcga cgatcactcg gggctactcg accaacggaa attgccagaa
     2221 aatcttatca cgcgattagg cgatattctc ccccacattt cggctatata ggatacaagt
     2281 ctcttccttc aatgatactt gtatcaagca attccctcga ccctgttgtc ttatcctgga
     2341 gtcatcacca cgtttccctt tttctgccct gtttcaacgt acttgtgagc atctacgact
     2401 tgctctaaag aatatcgcct gtcaatcact gtttttatct tccccgcttc gatcagttct
     2461 ttgagcgtgt tcaagtcttt tgtgctgcgg ttggtaaact gaaagaggac tttcttgctg
     2521 gtgaatcttg aagcccacgc ggcgcggatc attttgggca aggtcgggtt ggtcataaga
     2581 tagattccct gtgcattcag agacttcata atccgcgaaa acgaactcct caataccaca
     2641 tccagaatca catcatattt ctgaccattt ttagaaaaat cttctttgct gtagtcaatc
     2701 acatggtctg cgccaatgga gcgcatcata tccaatttcc ccgcactgtc cacagctgtg
     2761 acttctgctc catagtgttt ggcgagttga atggcatacg tgccaatgct cccgcccgcg
     2821 ccgttgatca ataacttctg tcccggttgt atgtttgcca tgcgcaggaa atgcagcgcc
     2881 tccatcccgc cgagagaaac gggcgcggct tcttcgtacg gcaggtccgc aggcatgggt
     2941 atcactgttt cttcaggcat gcagatatat tcagcgtatc cgccaaatcc cattccgctg
     3001 aacccaaaga cctgatcacc aattttgaag tgctttgtat ctttcccgac cacttcgact
     3061 tccccagcca aataaccccc aagaatgttt actctcctcg gttttctgaa acctacataa
     3121 attcgcatcg ggacgcgtac ccacaacgga aattgtagat tcctcatctc gcaatcccca
     3181 gtggatacca gcactgcacg aatctttatt aatacttcgt tatctttcgg cgcgggcttt
     3241 tccacctctt tcaagtgaag gacttccggt ggtccgtatt ttgtccatag aatcgctttc
     3301 atgatccctc atcctgattc gattttgttt tctacgttgc agcgacgatg ttcccattgt
     3361 tctgtacttc tttatgatac ctgatagatt cccgggtttc atcgaagtgg taatgtttat
     3421 tttcgagagt gtctaatttc gtgggcttag ccacagagac ctttgagttt tttctcagtg
     3481 aactcagtga tccacctgca ctccccggca ccgaccaacc ggggcggtgt gcgccaggtg
     3541 caagtgtctg tggcgaaaca gcttttagtc catacttttg gacactccct tattttcctg
     3601 gttcaagtac agtaacattc tgtcccgcgt acttttgata caagtcattg cgacgttttc
     3661 tccaggcgtc caggccgccg cgcaaattgc tgacgtgctt aaaacctttc gacttcaagt
     3721 aatacgagat catgaaggaa agacctccgc ccgggcaata tacgataatc tccttatctc
     3781 ttgggagttg atcgaggtat ttttcgacgg aaaggggatt cacttcatga gagttcggca
     3841 gacctttggc cttttttcgg tcatctggtg ggcgtacatc caggatgtag gtcccttgat
     3901 cgaacaggat ggtttcgttt agttgtgcgg gtgtgatcat gttgacatac ggagttcggc
     3961 cttccacata gtcttgaaat acctgagcca gatatccgat gtcgctcgtc gaggtgttgc
     4021 tcccgaggct gatacgaatg gtctcgcgcg cggcttgatc agatagtccc atcgctttca
     4081 aaacatgaga aggtttgtct tctcccgtgc tgcatgcgga gcctgccgaa acagcgatgc
     4141 cgcgatagtc cagcatcccc atcagcccgg cattcccgac acccggaaag gtaatattga
     4201 gcgtgttcgg caggcactcg gcctctgttt ctggggaatt aagcacacag tccggtttga
     4261 tctctttgag ccgcgtgatc aactgcccct tcaagtcgcg gacccgctgt gccttcgtca
     4321 gtagtttatc cacgtcctgg caggcggcgc ccaacccggc gatgttatgg atactttcgg
     4381 tgccagcgc
//
LOCUS       DASTJP010000223         4954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_670449, whole genome shotgun sequence.
ACCESSION   DASTJP010000223 DASTJP010000000
VERSION     DASTJP010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670449"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..594
                     /locus_tag="VFQ23_06855"
     CDS             202..594
                     /locus_tag="VFQ23_06855"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HET9906340.1"
                     /translation="MTTKPRFSEETIATLHKDRIMGIRAGGQSDHRVIGIWVVVVNGR
                     VFVRSYRMKQGGWWRTFLEDPYGEIFVSKRKRGIKVRAVQTRSEKLKDAVSAAYKEKY
                     NTRGSVGYVEEMSKKPLRDTTTELVAIK"
     gene            638..1795
                     /gene="tgt"
                     /locus_tag="VFQ23_06860"
     CDS             638..1795
                     /gene="tgt"
                     /locus_tag="VFQ23_06860"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9906341.1"
                     /translation="MFEFQITAKSNRARTGVFATPHGDLLTPVFAPVGTQATVKTLTP
                     EHLTDINASLVLSNTYHLYLRPGDELVRDMGGLHKFMQWQRPMLTDSGGFQVFSLAQT
                     RKIDDDGVTFKSHIDGSTHRFTPEKSIAIQENLGADIIMAFDECSDPNDHVYSKLAME
                     RTHRWAERSLQAQTRTDQALFGIVQGGVQADLRAESAAFIASLDTPGIAIGGLSVGET
                     KEEMHDTLDIVTPLLPENKPRYLMGVGTPEDLINGVARGIDIFDCVLPTRLARHNSAF
                     SPEGRLNLMNATFARDERPVDSTCDCYTCQTFTRAYIRHLIVAKELLAGTLLSIHNLR
                     ALIRLMEQIRCYIADGTFESRVPELLNQWSNNAKRKHATVGTQREDGSLES"
     gene            1826..2203
                     /locus_tag="VFQ23_06865"
     CDS             1826..2203
                     /locus_tag="VFQ23_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906342.1"
                     /translation="MPPTMHPDTAYQLFSDHLMHNETLTTKSGKGGLSYYKKGVFVCH
                     FNAKPRKDRDDLGFADFRYDALHPYLDIEKTISAIQDEATSEIEIRAHKLWCSLHFPL
                     SKLEQIAELFLRHIITKVNISLN"
     gene            2215..3018
                     /gene="uppP"
                     /locus_tag="VFQ23_06870"
     CDS             2215..3018
                     /gene="uppP"
                     /locus_tag="VFQ23_06870"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HET9906343.1"
                     /translation="MNILHAFILGIIQGLTEFIPVSSTAHLLIGQQLLNIPADDAMFS
                     FLVIVQLGTLISLFAFYWKDLLSIVRATLDFRRSTPERNLGIYIIVATIPALLAGLLL
                     KDAIEALFETPLLQASIRLFSAATLLTLAELLTKKNRSLNSMTWLDALIIGLFQILAV
                     FPGASRSGSTISGGLFRGFDRVSAARFAFLMSIPVMLAAGGYETLDLIQMPNLGEFLP
                     LLAVGFITAAVVGWFAIKWLIDYLSQRSLYIFAGYCAIVGSICLAIYLF"
     gene            3021..4121
                     /locus_tag="VFQ23_06875"
     CDS             3021..4121
                     /locus_tag="VFQ23_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET9906344.1"
                     /translation="MKHLLKTLVETESPSHDKAAVDRVGAIVAEEARKLGAQVGIIPN
                     KETGDHVLCRFDSSVGRETVSESPILFLCHMDTVFPLGTIQKTPFREDNDKIFGPGTL
                     DMKAGIVIALAAIEKAQESGLNRPVTLLCTSDEEIGSDTSRELIESLAKESALVLVME
                     GALLDGSLKTWRKGTGEFWVKTKGRAAHAGGDHQAGRNAIEEMAHQVIAIQKLTDYSK
                     QTTLNVGVIQGGTVSNVVPEEAVIQVDVRVMLPGEWERLGSEMQKLKPVLDGTSIEIT
                     GGLNRPPMPYDEKMKTTFEKAKSIAANIGVTLSAGGTGGASDGNFVAPLGIPLLDGMG
                     AVGEGYHSDREYIFAGSLEQKATLIATLLREW"
     gene            4127..4867
                     /locus_tag="VFQ23_06880"
     CDS             4127..4867
                     /locus_tag="VFQ23_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906345.1"
                     /translation="MSLRGANRSAVSDVAISAPIKNLLYQLCRVCSPFSARVSFFLLS
                     SFILFSCSTSTPLATPQLVTVYSTSAAQPWLRDLYACAAQQNNVALSRIDDSSQAEIS
                     LRVGEPEILSSPSYQIDTEEILIVTHRTSPIQNLTLDEARTLFAGQGDPSVQLWTYSS
                     GEDVQEVFDQVVMEGRSVSSSAWIAAHPQQMSDTLVNEPNTVGILPRHWKMGDSRLVY
                     TVPNIPVLAVTPNEPEGAVRDLIACLQK"
BASE COUNT         1234 a         1199 c         1232 g         1289 t
ORIGIN      
        1 cttcttccag cattatttga tttccctgtt ccttgccgag atattgatct ggattattga
       61 aagtgcaatg ctatattacg tcccggcgaa caagctctta ttacaggaag ctacatttct
      121 gagcctgctg atgaatttgg cgagttttgg gatcggatgg tttttgccga cctaatttac
      181 caactccaaa gggatttcct tatgaccacc aaaccacgat tcagtgaaga aactatcgca
      241 acgctgcata aagacagaat tatgggcatc cgcgccgggg ggcaatccga tcatcgcgtc
      301 atcggtatct gggtcgtcgt tgtgaatggg cgtgtctttg tccgttctta caggatgaaa
      361 caaggaggct ggtggcggac gtttctcgaa gacccgtatg gggagatctt tgtttccaag
      421 cgaaagcggg gaattaaagt ccgtgcagtg caaacgagaa gtgagaaatt gaaggatgca
      481 gtcagcgccg cctataagga gaaatacaac acgcgagggt ccgttgggta tgtggaggaa
      541 atgtctaaaa aaccgctgag ggataccacg acggagttgg tggccataaa gtaaaagatt
      601 tcctgtctcg aataacgagt atcgtataat tccccggatg tttgaattcc aaataacagc
      661 caaaagcaac cgcgcccgca caggcgtgtt tgccacacct catggtgatt tgctaacgcc
      721 ggttttcgcg ccggtgggga cccaagccac ggtcaaaact cttactcccg aacatctcac
      781 agacatcaat gcctcacttg tgttgagtaa tacctatcat ctttatctgc gtcctggtga
      841 tgagcttgta cgagacatgg gcggtttgca taagttcatg cagtggcagc gtccgatgct
      901 gactgactcg ggcggttttc aggttttctc gctggcacag actcgtaaaa ttgacgatga
      961 cggtgtgacc ttcaagagtc atattgacgg ctcaacacat cgtttcactc ccgaaaagtc
     1021 gattgccatt caggagaatc tcggggcaga cattatcatg gcgttcgatg agtgctcgga
     1081 ccccaacgac cacgtctaca gcaagcttgc catggaacgc acccatcgct gggcggagcg
     1141 ttctcttcaa gcgcaaacgc gaactgatca ggctctattc ggcattgtac aaggtggcgt
     1201 ccaggcggac cttcgggcgg agtctgctgc atttatcgca tctctcgata cgcccggaat
     1261 cgccattggc ggactctccg tgggcgagac caaagaagag atgcatgata cgctcgacat
     1321 cgtcactcca ttattgcctg aaaacaagcc gcgctacctg atgggtgtcg gtactcccga
     1381 agacctgatc aacggcgtcg cacgcggaat tgacatcttt gactgtgttc taccgacccg
     1441 cttagcccgc cacaactctg ccttttcacc cgaggggcgg ctcaacctga tgaatgcaac
     1501 ctttgctcgc gatgaacgtc ctgtcgacag cacatgtgat tgttacacgt gccagacatt
     1561 tacgcgagcc tatatccgtc acctcattgt ggcgaaagaa ttgcttgcag gaacattact
     1621 atctattcat aatttgcggg cattgataag attaatggag caaatcagat gctatatcgc
     1681 agatgggaca tttgagtcgc gtgtacctga gttattgaac caatggtcga ataacgcaaa
     1741 acgaaaacat gcaactgtag ggacacagcg ggaagatgga tcgttagaat cctagatggt
     1801 ctcgctgtgt ccctactaca acggaatgcc tccgaccatg caccctgaca ctgcttacca
     1861 actcttctca gaccatctta tgcataatga aacacttacg acaaagagtg gcaaaggagg
     1921 tttgagttat tacaaaaagg gtgtctttgt ctgccacttc aacgccaaac cgcgaaaaga
     1981 tcgcgatgat cttggatttg cagatttccg ttatgatgca ttgcacccgt atcttgatat
     2041 tgaaaaaacc atttctgcaa ttcaagatga agccacctct gaaatagaaa ttagagctca
     2101 taaactctgg tgtagtctgc attttccgtt atcgaaattg gaacagatag ccgagctgtt
     2161 tttaagacat attattacca aagtaaatat ttcattgaat taggagaatc atctttgaac
     2221 attcttcacg cctttattct tggcatcatt caaggactga cagaattcat tcccgtatct
     2281 tccaccgcac atttgctgat cgggcaacaa ctacttaata tcccggctga tgatgcaatg
     2341 ttctcattct tagtcatcgt tcagttgggg actttgattt ccctctttgc cttctattgg
     2401 aaggatctat tatccatagt cagggcaacg ctggattttc gccgttcgac tccggaaagg
     2461 aatttaggca tttacatcat tgttgccaca attccagcgc ttttagccgg cctgcttctg
     2521 aaggacgcca ttgaggcgtt attcgaaact cccttgcttc aagcctccat tcgcctattc
     2581 tctgctgcaa ctctactaac cctggctgaa ttgctcacaa aaaaaaatcg cagtttgaat
     2641 tcaatgacct ggctggatgc gctgatcatc ggcctgtttc agattcttgc cgtcttcccg
     2701 ggtgcctcac gcagtggctc gacgatctct ggcggtctgt tccgcggctt cgaccgggta
     2761 tccgcggcac ggttcgcgtt tctcatgtcc atacctgtga tgctggctgc agggggatac
     2821 gaaacgctgg atttgattca aatgccgaat ttaggtgaat tccttcctct acttgctgtg
     2881 ggatttatca ctgcagctgt ggtcggctgg tttgccatca agtggctgat tgattatctg
     2941 agccaacgtt ctttgtatat ctttgccgga tactgcgcga tcgtcggcag catctgcctc
     3001 gcaatctacc ttttttgatc atgaagcatt tactaaaaac tctcgtcgaa accgaatcgc
     3061 cttcgcatga caaagcggca gttgatcgcg tcggtgcgat tgttgccgaa gaagcgcgta
     3121 agcttggtgc gcaggttgga atcatcccaa acaaagaaac cggtgaccat gttttatgta
     3181 gattcgactc ctccgtcggg agagagactg taagtgaaag tccgattctg ttcctgtgcc
     3241 acatggatac ggtttttcct ctcggcacga ttcagaagac tccttttcgt gaagacaacg
     3301 ataagatatt tggtccgggc acattagata tgaaagccgg aattgtcatc gctttggctg
     3361 ccattgaaaa ggcacaagag tcggggttga atcgaccagt cacgttactt tgtacgtccg
     3421 atgaagaaat cggcagcgat acatcccgcg agctaattga atcgctcgcg aaagagtcgg
     3481 cgttagtatt ggtgatggaa ggagcgttgc tggatggctc gctcaagaca tggcgtaaag
     3541 ggaccggaga attctgggtc aagaccaaag gacgcgctgc ccatgccggc ggtgatcatc
     3601 aggcagggcg aaatgctatc gaagagatgg cgcatcaggt aattgccatc cagaaactta
     3661 cagactattc aaaacaaacc acgctcaatg ttggcgtcat tcagggaggc acggtttcga
     3721 atgttgttcc cgaagaagca gtcattcaag tggacgtccg tgtgatgctg cccggcgaat
     3781 gggaacgact cggatctgaa atgcaaaagc tcaagcctgt gctcgatggt acatccattg
     3841 agatcacagg tgggttgaat cgtcctccga tgccatacga tgaaaaaatg aagactacgt
     3901 ttgagaaagc aaagtctata gcagcgaata ttggcgtaac tctctccgca ggtggaacgg
     3961 gtggcgcctc cgatggaaac tttgttgccc ctttgggcat tcccttgctg gacggcatgg
     4021 gcgcagtggg ggagggttat cactcagatc gtgaatatat tttcgccggg agcctggaac
     4081 aaaaagcgac tctgatcgca acgttgcttc gggagtggtg ataacgatgt ctttgcgagg
     4141 agcgaaccgc tctgcggtaa gcgacgtggc aatctccgcg cctataaaga atttacttta
     4201 tcaattgtgc cgcgtctgct ctccgttcag cgcgagagtt tctttcttcc ttctatcctc
     4261 tttcatcctc ttctcctgct cgacaagtac acctctcgcg acgcctcaac ttgtcaccgt
     4321 ttattcaacg tcagccgcac agccgtggct acgtgatttg tatgcttgtg cagcacaaca
     4381 gaataatgta gccttgtcta gaatcgatga ttcatctcag gctgaaatct cgctgcgtgt
     4441 aggagagcca gagatactat cgtcaccttc gtatcaaatt gatacagaag agattttgat
     4501 tgtcacacat cgaactagtc caattcaaaa cttaacgctt gacgaagcac gcaccctgtt
     4561 cgcggggcag ggagatcctt cggtgcagct atggacgtac agctcaggtg aggatgtaca
     4621 ggaggtgttt gaccaggttg tgatggaggg aagaagtgtc tcatcatcgg catggatagc
     4681 ggcccacccg caacagatgt ctgacacctt agtcaatgaa cccaacaccg tcggcatatt
     4741 gcctcggcat tggaagatgg gggactcacg cctggtatat accgtaccaa atattcctgt
     4801 gctggcagtc acgccgaatg aaccggaagg tgccgtccgg gatttgatcg cctgtctaca
     4861 gaaataactt caaaaattgc cagcccggct cgcgtgagtc gggctttttt tatttatctt
     4921 gacagcctat gcaaatggga gtaatatagt tgca
//
LOCUS       DASTJP010000224         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_131860, whole genome shotgun sequence.
ACCESSION   DASTJP010000224 DASTJP010000000
VERSION     DASTJP010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131860"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="VFQ23_06885"
     CDS             <1..1559
                     /locus_tag="VFQ23_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9906346.1"
                     /translation="TPAYQLLFAEPDSTQPVIQTGTYQLEISSLFFEENSDLNPQLVL
                     LGQVYGLAGTDFWRRDLVVPLFWGMPFVLFVGLLGTFITTLVAIILPAIGVWYGGWLD
                     DLIQRLTEVNMVLPGLAIVVLTNLLFGVNVWIVLGIIVVLNAFGSPIKSFRSAFLQAK
                     EAPYIEMARSYGASDFRIITRYLVPRILPVFIPQLVTQVPSFIFLEATLGFFNINSHY
                     PSWGRIIYDGLAHGAIYGSPFWVLEPIFLLLLTGLAFAMLGSALERILNPRIISDVPL
                     AGDKEKSNERKSWGSKLNRRLIAAAIVSLLVIAIFVPTIQGKSLASFVIHFMDETRNL
                     HTTNLKPPVIPTRTSLPAASTPTDMPASATEPVTTPTIPVTFTPTTQASCIPANRPAT
                     AKVLEVIDGNTIKVLMDGLVYVVRYIGVELPQDDGAAMDAYLVNSELVFRKEVTLIGD
                     RMYKDPSGRLLRYVLVGDTFVNLWLLENGWGEAVDLPPNSSCTRALKSAQELAMQSAV
                     GIWAVPTPSP"
     gene            1609..>3003
                     /locus_tag="VFQ23_06890"
     CDS             1609..>3003
                     /locus_tag="VFQ23_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906347.1"
                     /translation="MFREIFTQRIKRGVVRRLNNFKIASKAEEVARKEPQPSGAPVIF
                     FKASTGIDDLSWNSGFHLLASWALRLKGIPVVYFACQSGMSHCVLGTNRDNAKKAPPC
                     KSCISQSKTLYTNVRTFERSNVSTAGVQWFDFQRDEQLASALENLSVDELSKFEWNLK
                     IKDVAANRSLAKQSPVNEEIASSQSALFAMTLPLGSLCLPGLRWILRIHHLNDDENTR
                     YLLREYILSAWNVAQKFSSLLDETNPRAVIVFNGQFYPEATARHIAQKRGIRVITHEV
                     GLQPASAFFTDREATAYPIHIPETFELNHEQNAKLDAYLAKRFQGDFTMAGIKFWADM
                     KGLDESFLQKTAGFKQIVPIFTNVIFDTSQPHANTVFEDMFDWLDMALEVIREHRETL
                     FVIRAHPDELRVRKSSRETVAAWVETSGANQEPNVVFVAPNETLSSYELIQKSKFVMV
                     YNSTIGLEASIMGAP"
BASE COUNT          715 a          705 c          744 g          839 t
ORIGIN      
        1 ccacacctgc ttaccagctt ttgtttgcag aaccagattc cacacagcct gtgatacaga
       61 ctggcaccta tcaattggag atcagtagtt tattctttga agagaattct gatctgaatc
      121 cacagttggt tcttctgggg caggtgtatg gattggccgg caccgatttt tggcgacgcg
      181 acctggttgt gccgttattc tggggcatgc ctttcgtcct tttcgttggt ttattaggta
      241 cgtttattac tacactcgtt gctatcatcc tgccggcaat tggggtttgg tatggtggct
      301 ggctcgacga tttgatccag cggctgaccg aagtcaatat ggtattgccg ggtctggcga
      361 ttgtagtgct gaccaacctg ctgtttggcg ttaacgtttg gatcgtattg ggcataatcg
      421 tcgtcctaaa tgcttttggg tcgcccatca aatccttccg ttccgcgttc ctgcaggcaa
      481 aagaagcacc ttatattgaa atggcacgtt cgtatggtgc aagcgatttt cggatcatta
      541 cacgctatct tgtaccacgc atcctgccgg ttttcattcc acaacttgtc actcaggttc
      601 ccagtttcat ttttcttgaa gccaccctcg gctttttcaa cattaattct cattacccat
      661 cctggggacg tatcatttat gacggactgg cacatggcgc aatctatggg tcaccgttct
      721 gggttctgga accgatcttc ctgctattgt tgactggtct cgcgtttgcc atgcttggtt
      781 cggcactcga acggattctc aacccacgca ttatttcaga tgttccactt gccggtgaca
      841 aagaaaagtc caatgaacgc aaaagctggg gatccaaact caatcggcga ttgatcgccg
      901 ccgcgattgt aagtctgtta gtgatcgcca tatttgttcc gaccattcag gggaaatctc
      961 tagccagctt tgtgatccat tttatggatg agactcgaaa tctgcataca acaaatctga
     1021 agccgcccgt gattccgacg cgtacatctt tgcctgccgc atccactccg actgatatgc
     1081 ctgcttctgc aacagaaccc gtaacgactc ccacaattcc ggtcaccttc acaccaacca
     1141 cacaggcatc ttgtattccc gcgaatcggc cagccactgc aaaagtcctt gaggtcattg
     1201 atggaaatac gatcaaggtc ttgatggatg ggcttgttta tgtcgtccgc tatatcgggg
     1261 tggagctacc tcaggacgat ggggctgcta tggacgcata ccttgttaac tcagagcttg
     1321 tttttcgtaa agaggttacc ctaatcggcg accgcatgta taaagacccg agtgggcgat
     1381 tattacgtta tgttttagtt ggagatacct ttgtaaatct ttggcttctt gagaatgggt
     1441 ggggcgaagc cgttgattta ccgcccaatt catcttgcac gcgggcactc aagtcggctc
     1501 aagaactcgc gatgcaatcc gcagtgggga tttgggcggt tccgactccc tctccctaaa
     1561 ttttgggagt ggctaagtaa ataagaattg ctgataagat tgggtgaaat gtttcgagaa
     1621 attttcaccc aacgcatcaa acgcggagtg gttcgcagac tcaataattt caaaattgca
     1681 tcgaaagctg aggaggtagc tcgaaaagag ccacagccat cgggcgcgcc ggtcatcttt
     1741 ttcaaagcat ccacggggat tgatgatctc tcctggaaca gtggttttca tctacttgca
     1801 tcctgggcat tgcggttaaa ggggatccca gtcgtttatt tcgcgtgtca atcagggatg
     1861 agtcattgcg tgctggggac gaatcgggat aatgcgaaga aggcgccacc gtgtaagtcc
     1921 tgtatttctc aatcgaaaac tttgtataca aacgttcgaa cgttcgaacg ttcgaacgtt
     1981 tcaacggcag gtgttcaatg gtttgatttt cagcgcgatg aacagttggc aagtgctttg
     2041 gaaaacttaa gtgttgatga attatccaag tttgagtgga atttaaaaat aaaagatgtc
     2101 gctgcgaacc gctctttggc gaagcagtct cctgttaatg aggagattgc ttcgtcgcaa
     2161 agtgcgctct tcgcaatgac attaccattg ggctcattat gcctaccggg cttacgttgg
     2221 attcttcgta ttcatcatct aaatgatgat gaaaatacgc gttatcttct gcgagaatac
     2281 atcttatcgg catggaatgt ggcacagaag ttttcatcac ttctggatga aacaaatcca
     2341 cgcgcggtca tcgtgttcaa tggacagttc tatccggagg caaccgcgcg tcatatcgcc
     2401 caaaagcgtg ggatccgtgt gattactcat gaagtggggc tacaaccggc ctccgcgttt
     2461 ttcacggatc gcgaagcgac ggcatacccg atccatatcc ctgagacgtt tgagttgaac
     2521 catgaacaaa acgcaaagct cgacgcgtat cttgcgaagc gctttcaagg tgacttcacg
     2581 atggcgggca tcaaattctg ggcagatatg aagggactgg atgagtcgtt tttacagaaa
     2641 acagcaggct ttaaacagat cgtgcctatc tttaccaatg tcatctttga taccagtcag
     2701 ccacatgcta atacggtgtt tgaagatatg ttcgactggc tcgatatggc tctggaggtc
     2761 attcgcgaac atcgcgaaac tctcttcgta atccgtgcac atcctgatga gctacgcgtg
     2821 aggaaatcca gccgtgagac ggtcgccgcg tgggttgaaa ccagcggtgc gaatcaagag
     2881 ccgaatgtag tctttgtcgc gccaaatgaa acattgagtt cgtacgagtt aattcagaag
     2941 tcaaagttcg tgatggtcta taactcaacg atcgggttgg aggcttcgat tatgggcgcg
     3001 cct
//
LOCUS       DASTJP010000225         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1432397, whole genome shotgun sequence.
ACCESSION   DASTJP010000225 DASTJP010000000
VERSION     DASTJP010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432397"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFQ23_06895"
     CDS             complement(<1..203)
                     /locus_tag="VFQ23_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906348.1"
                     /translation="MQIILDKIRALSPYQQLLNDLQAHKELPGLGLPRAARLPVLAAL
                     HQDIAAPILLVTDRSDHALSLFD"
     gene            complement(290..874)
                     /gene="pth"
                     /locus_tag="VFQ23_06900"
     CDS             complement(290..874)
                     /gene="pth"
                     /locus_tag="VFQ23_06900"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET9906349.1"
                     /translation="MTLDTFLLIGLGNPGREYRDTRHNFGFMLVDRLAVRLNARGMKM
                     QSKAIVTDAMYEDHKLILAKPQTYMNLSGPSVQGLVHFYKIPLTNVMVLSDDLDIPFG
                     TIRIRASGGYGGQRGLASIIESLGTKDFPRMRLGVGRPPGKMDPANFILQNFSRDEIK
                     AISEILDAGADAVLEFVKNGLNASMNKFNGNVND"
     gene            complement(892..1497)
                     /locus_tag="VFQ23_06905"
     CDS             complement(892..1497)
                     /locus_tag="VFQ23_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906350.1"
                     /translation="MKLRFLFPIVLLLIAAQDEPPIAITSPLANDVLRGEITITGTTD
                     IPNFFSAQLDFAYASDPTGTWFALQTISLPIRDATLATWNTASISDGDYILRLRVNLM
                     DNTVQEVTVPIQIGNDVAPVTPTVVPTATLNAVEVQIPTPFLLAASPTPTAPPRPTPT
                     ALPSNPASLGQTAIYTSLGRGALVILGLFAFSGLLLRIRRF"
     gene            1631..2005
                     /locus_tag="VFQ23_06910"
     CDS             1631..2005
                     /locus_tag="VFQ23_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9906351.1"
                     /translation="MTRNRADQMVERLRNMQAAAPDIEASAVVSVDGLIMASALQQGV
                     EEDRVSAMSAAMLSLGERISTELGRKGLEQVYIKGNKGAIVLTSIGEEAVLTALARQD
                     AKLGMIFLEMRRAAEDLVKLVG"
     gene            2169..>3231
                     /locus_tag="VFQ23_06915"
     CDS             2169..>3231
                     /locus_tag="VFQ23_06915"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9906352.1"
                     /translation="MTTKYLFYTGGVVSSVGKGVTAAATGLLLKERGFKVAVQKLDPY
                     INVDPGTMSPYQHGEVYVLDDGAETDLDLGHYERFIDIRLSRSSNFTSGQVYAEIISK
                     ERRGDFLGGTIQVIPHITNEIKRRVASIGKETGADVVIVEVGGTVGDIESQPFLEALR
                     QLRNEFGRENVYFIHVTWLPYIGATGEIKTKPTQHSVAALRSIGISPNMIIARSDYPV
                     DKELCDKIALFCDVEKEAVVPMITSDVLYEVPLLLEKAGVGDYLVEKLNMKATRKPDM
                     KPWEKLVERVRKPKPTVKVALVGKYVELQDAYMSVREALKHAALANDVEVEIGWVHSA
                     DLEKDKGWEVIQNSDGILVP"
BASE COUNT          836 a          805 c          899 g          691 t
ORIGIN      
        1 tcatcgaata acgacaacgc atgatccgag cggtccgtga cgagcaggat gggagcagct
       61 atatcctgat gcagggccgc aagcacgggt aagcgcgccg cacgcggcaa ccccagaccc
      121 ggaagttcct tatgagcctg caggtcgttc agcaattgct gatatggaga gagagcgcga
      181 attttatcga gaatgatttg catttcttat tactggtggt ctcgacactt gcacctgcgt
      241 gccagtgcag gtgtacagcg gacgatcacg cctactcgac caccgatggt taatcattta
      301 cattgccatt aaacttattc atcgaagcat tcaagccgtt cttcacaaat tcaagcacgg
      361 catctgcacc ggcatccagt atttcagaaa tcgccttgat ctcatcgcgc gagaaatttt
      421 gtagaatgaa attggcaggg tccattttgc cgggtggacg acccacaccc agccgcatgc
      481 gcggaaagtc ttttgttccc aggctttcga tgatggaagc cagaccgcgc tgcccgccat
      541 aaccacccga ggcacggatg cgaattgtcc caaagggaat atccaggtca tcagaaagca
      601 ccatgacatt ggtcagcgga attttgtaaa agtgaaccaa tccctgaacc gatggaccgg
      661 agagattcat ataggtctgc ggtttggcta gaattaactt gtggtcttcg tacattgcat
      721 cggtgacaat cgccttcgat tgcatcttca taccgcgcgc gttgaggcga accgccaggc
      781 ggtcaaccag catgaaccca aaattgtgac gggtatctct gtattcacgg cccgggttgc
      841 cgagtccgat gaggaggaag gtgtcaagtg tcatatcaaa agttccagat ttcagaaacg
      901 gcgaattcga agcagaagac ccgaaaaggc aaataaacca agaataacca gtgcaccacg
      961 acctaggcta gtatagattg ctgtttgccc caacgaggcc ggattgctcg gcaacgccgt
     1021 cggtgtgggg cgcggcggag cagtgggtgt cggtgacgcc gccagcagaa acggcgtagg
     1081 gatttgaact tccacggcat tcaacgtagc tgtgggaacc accgtgggtg tgaccggcgc
     1141 cacatcattt ccaatttgaa ttggaactgt gacctcctga accgtattgt ccataagatt
     1201 tacccgcaat cgcagtatgt aatcaccatc gctgatggag gcagtattcc aggtagctaa
     1261 cgtcgcatct cgtatgggca gcgaaatggt ctggagggca aaccatgtgc cggtggggtc
     1321 tgacgcatat gcgaaatcca attgcgcgga gaagaagttt gggatgtcgg tcgtgccggt
     1381 aattgtgatt tccccgcgta aaacgtcgtt tgccagcgga gatgtgatgg caatcggggg
     1441 ttcgtcttgc gcggcaatca acagcaaaac aatcgggaaa aggaagcgca atttcatcaa
     1501 tcgaacgtac aagtttaaca taaagcgaaa cgacggtcaa attaggcgta atcttcgata
     1561 atctgataga gctattctcc tgaaatttga agtatggtaa actgtgacag acttcaacta
     1621 ggagaaccgt atgacaagaa atcgcgctga ccaaatggtg gaacgactgc gcaatatgca
     1681 agccgcagcg cccgatattg aggcttcagc agtcgtctcg gtcgatggac ttatcatggc
     1741 ttcggctttg caacagggtg tggaggaaga tcgtgtctct gccatgtctg ccgctatgct
     1801 cagcctcggc gaacgcattt ccacggagct cggacgcaaa ggactcgagc aggtttacat
     1861 taagggcaac aaaggcgcga tcgtgctgac atccattgga gaggaagctg ttctaaccgc
     1921 gctggcgcgg caggacgcga aattaggtat gatcttcctc gagatgcggc gtgccgcaga
     1981 agatctcgtc aagctggtcg gatgaaaaag ataacgacca gcaactcaca aagtacgcaa
     2041 tccccgatgg ggagggctta accgccctcc ccatctcatt taacccctcc cgccccttcg
     2101 gcgcaccctc cccattttcc aaaggaaaat ggggagggct ggggtggggc aattcattgg
     2161 agtaaaaaat gaccacgaaa tatttatttt atacaggcgg cgttgtttcg tcagttggca
     2221 aaggtgtgac agccgcggca accggactct tgctaaagga gcgcgggttc aaagtggcgg
     2281 tgcaaaaact ggatccatac atcaatgttg accctggcac gatgagccca tatcagcatg
     2341 gagaggtcta cgttttggat gacggcgcgg aaacggacct tgacctgggt cactacgagc
     2401 gcttcatcga catccgcctc agccgctcca gcaacttcac cagcggacag gtctatgcag
     2461 agatcatcag caaggagcgc cgcggcgatt tcctgggtgg gacgatacag gtcatccctc
     2521 acatcacaaa cgagatcaag cgtcgggtgg cctcgatcgg gaaagagacg ggcgcagatg
     2581 tggtgattgt ggaagtcggc gggacggtcg gtgacatcga gtcgcaacca ttccttgaag
     2641 ccttgcggca actccgaaac gaattcggtc gcgagaatgt gtacttcatc cacgtgacct
     2701 ggcttccgta tatcggggcg acgggcgaga tcaaaaccaa gccaacacag cactctgtcg
     2761 ccgcgctgcg ctcgatcggt atctcgccaa atatgatcat tgcccgctca gactatccgg
     2821 tcgataaaga gctttgcgac aagatcgcct tgttctgtga tgtggaaaaa gaggcggtcg
     2881 tcccaatgat cacaagtgat gtcttatacg aggtacctct cctgttggaa aaagcaggag
     2941 tcggtgatta tctggtggaa aaactcaata tgaaggccac acgcaagccc gatatgaaac
     3001 catgggaaaa actggtggag cgcgtgagga agccgaaacc caccgtcaaa gtggcgttag
     3061 tgggcaaata cgttgaattg caggacgctt acatgtcggt gcgtgaagct ctaaaacatg
     3121 ctgcgctcgc caatgatgtg gaagtggaaa tcggctgggt gcattcggct gaccttgaaa
     3181 aagacaaggg ctgggaggtc atccaaaact cagatggcat cctcgtcccc g
//
LOCUS       DASTJP010000226         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_658721, whole genome shotgun sequence.
ACCESSION   DASTJP010000226 DASTJP010000000
VERSION     DASTJP010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658721"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VFQ23_06920"
     CDS             complement(<1..1114)
                     /locus_tag="VFQ23_06920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9906353.1"
                     /translation="MALIAKELARLEKFDVTMVVADHGQPHIEKRADVALYSWEGRSI
                     WGIPSRDEQLGDPKNGSKPAESFSARLGRRLNQFRLSLIRSSLTGQVGDYGITPNMIS
                     IYSEVDADIYIVPGNSEYSTEVAFYCQQRGKRYVFLAGSDIDYSPEYKLHPEKQDIYG
                     LSYFLKTYAIESADAHIVQNEHQASLLQNGYDRTGTVIKNPIDIDRKFPRHAAKGTLL
                     WVGKSDEVIKRPSLILELARQLPQFKLVMIMPSVNDEIHSKLLGEANELSNVTLLEYV
                     PFKQIESHFASAYLHVNTSTFEGFPNTFLQAAKYGVPTVSLKVDPGGMLSQHHCGVAC
                     MDDFDTFKQTVQALMSDDQLHESYSRQSLEYVKKNHD"
     gene            complement(1241..2908)
                     /locus_tag="VFQ23_06925"
     CDS             complement(1241..2908)
                     /locus_tag="VFQ23_06925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9906354.1"
                     /translation="MKIGILGNTNNYPLMLATQLLKAGHEVVLVITENTLLNRPEGRY
                     PEFKNKYPAWIIDASHLTEWDFISLNPILGSILDQISDCDGFILNSYCVSLLPLLGSP
                     GIAFLTGSDLSHYANFDSVQYRTHSWDVGYAALPDAKLKLKELYSFVQRQREGIQQAA
                     AVRYMPRGIVPSDDKLLEEIGVPSSKRIFIAPAELERVKLRPAPLNQPIRAFCGARIT
                     WKGPIEPGRSSLDYKGSDIMIRGLGQFYRETGTRLDVHLVRKGLHIAELQDIIDEEGI
                     TDQITWSDEMSLTELWEEFANSDIVFDQLGNAAIGMVGLDAMATGRPVIGNAQREMFE
                     DLFGEPSPVCQASTPDEVCAQLKRLVDDPAERERVGLAGRNYMEKYFNPKNAAKQIIE
                     ILQNALLNSEAHISRGIAHSYYLQKINAIQQELLSTQQEQNLEVNKKIQNEIPAQVLG
                     KWITNKNGGKSFERLRGPFQQQDKLCWIFNFPAPRLKSDSIRSPYESNLLLFEEDELL
                     GPAHAEHEEIRRTGGGRYSHWESSLYFSTSDGSDPNTNGRTYRIVWL"
BASE COUNT          835 a          711 c          647 g          884 t
ORIGIN      
        1 tatcatgatt cttcttcaca tattccagag actgcctgct ataactttca tgcaattggt
       61 catctgacat gagagcctgg acagtctgct tgaatgtatc gaaatcatcc atgcaagcca
      121 caccacaatg atgttgagaa agcatgccac ccgggtcaac tttaagtgat accgtcggaa
      181 ccccgtattt ggctgcttgc aggaacgtat tgggaaagcc ttcgaaagtt gaagtgttca
      241 catgcagata agcactggca aagtgacttt caatctgctt gaatggcaca tactccagca
      301 aagtaacatt actaagctca tttgcttctc cgagtagctt gctatgtatt tcatcgttca
      361 cggacggcat aatcataacc aacttaaatt ggggtaattg acgagctagt tcaaggataa
      421 gcgatgggcg ctttattacc tcgtcggatt tgcccaccca aagaagtgtc cctttcgctg
      481 catggcgggg aaattttcga tcgatgtcaa tcgggttttt aatcacggta ccagttcgat
      541 catacccatt ttgaagcaag ctggcttgat gttcattttg gacaatatga gcatcagcac
      601 tttctattgc ataggtcttt aagaaatagg ataggccata aatatcctgt ttctcagggt
      661 gtaacttgta ctctggagag taatctatgt cggaccccgc caggaagaca tatctttttc
      721 cgcgctgttg acaatagaaa gctacttctg tggaatactc tgaattgcca ggcacgatat
      781 agatatcggc gtcaacttca gaatagattg agatcatgtt gggtgttatg ccgtaatcgc
      841 ctacttgccc tgtcagggat gatcgaataa gtgacagccg gaattgatta agacgtcgtc
      901 caagacgagc gctgaacgat tctgccggct ttgaaccatt ttttgggtct cccaattgtt
      961 catcccggga tggaatgccc cagatcgacc tgccttccca tgagtatagg gctacgtcag
     1021 ctcgtttttc aatatgtggc tgtccatgat cggctactac catagtgaca tcaaacttct
     1081 ccaggcgcgc cagttctttg gcaatcaatg ccatgcgaac ctcccagcct ccgaaagaag
     1141 tttcatattt ggagttgaaa agcgggtata taaaaggggc ggtgaaacaa atacgaagtt
     1201 ttcggaacgg cttaagagga ttgatcattg gacgtaagca tcaaagccag acaattctat
     1261 aagttctacc gttggtattc gggtctgaac catcggaggt ggagaagtac aaactcgatt
     1321 cccaatgaga ataccgtccg cccccagttc tacgaatttc ttcatgttca gcgtgggcgg
     1381 gccccaataa ttcatcttcc tcaaatagta agagattgga ttcgtatggt gaacgaatcg
     1441 aatcactctt aagccgaggg gcaggaaaat tgaagatcca gcacaattta tcctgctgct
     1501 gaaatggtcc tcgaagtctt tcaaaacttt ttcctccatt tttattcgtg atccatttgc
     1561 ccaatacctg agcaggaatt tcgttctgta ttttcttatt cacttccaaa ttttgctctt
     1621 gttgtgtact taataattcc tgctggatag cattaatctt ttgtagataa taggaatgag
     1681 cgatcccgcg agagatgtga gcttctgagt taagaagtgc attttgtagt atttcaataa
     1741 tctgctttgc agcatttttc gggttgaaat atttttccat gtagttacga cctgccaacc
     1801 ctacccgttc acgctcagcc ggatcatcaa ctaatctctt aagctgggca cagacttcgt
     1861 ctggtgtgct tgcctgacaa accggcgatg gctctccaaa taaatcttcg aacatttctc
     1921 gttgagcatt tccaatgact gggcgcccgg tagccatagc atccagcccc accatgccaa
     1981 tcgcagcatt ccctaattgg tcaaagacga tatccgaatt ggcaaattct tcccaaagtt
     2041 ctgtgagtga catttcgtcc gaccaggtaa tctgatcagt gatcccttct tcgtcaataa
     2101 tatcctgcaa ttcagcaata tgcaggccct ttctcactaa gtgtacatcc agccgggtac
     2161 cggtctctcg atagaactgc cctaagccgc ggatcatgat atccgatccc ttgtaatcca
     2221 gagaagatct cccaggctca atcggaccct tccacgttat gcgagcacca caaaaagcgc
     2281 gaattggttg gttaagtgga gcaggcctca atttaactcg ttctaattct gccggagcaa
     2341 tgaaaattcg cttggaactt ggaacaccta tctcttcaag caatttgtca tcagagggaa
     2401 ctatacctcg aggcatatat cttactgctg ctgcctgttg aatcccttca cgttgtcgct
     2461 ggacaaaaga atacaactcc tttagtttca attttgcgtc aggcaaggca gcataaccaa
     2521 catcccaact atgtgtccta tattgcacac tatcaaagtt cgcataatgc gaaagatctg
     2581 agccggtgag gaaagcaatt cctggagaac caagaagtgg tagaagagaa acgcaataag
     2641 aattaagaat aaatccatca caatcagata tttgatccag gatcgatccc aaaatgggat
     2701 ttaggctaat aaagtcccac tctgtcaaat gcgacgcatc aatgatccac gcaggatatt
     2761 tatttttgaa ttcaggatac ctaccctcag ggcgatttaa aagagtattt tctgttatta
     2821 caagaactac ctcgtgtcca gctttaagca gttgtgttgc taacatcagt gggtaattat
     2881 tagtatttcc caggatgccg attttcattc tagattatgc tcctatgcta tttcgcttta
     2941 gtctgcttct cttcgagaaa tagtcgctcc aattccaaca gtctgtcaga gcgatcttta
     3001 ataaatctgg caggaactcc tccgtagatt ttaaaaggtt cgcaatccct gtttatgagt
     3061 gacaatgccc ctatgca
//
LOCUS       DASTJP010000227         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1376670, whole genome shotgun sequence.
ACCESSION   DASTJP010000227 DASTJP010000000
VERSION     DASTJP010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376670"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3048
                     /locus_tag="VFQ23_06930"
     CDS             <1..>3048
                     /locus_tag="VFQ23_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9906355.1"
                     /translation="WEEDFSLVKKRVDILQKEGVNNFRDYFSTHPDQVAELATLVRLV
                     DANQAAMKLSGVEWKDDLLKSPSNPISNGANLNFQEELLGLMSPRMGFKMERIQRNAK
                     GEQLQVLVSGRIPYGYEADWSKVIVSISDISDRKRMEDSLRDREQRLNAIIENEPECI
                     KLLDSDGTVLQMNAAGLEMIEADGPEDVLGKPIYSIVTEEFRQEFQNLTEKVLRGESG
                     KLQFEIVGLKGTHRWLDTHAVPMRNADGEVFALLGITRNITEYKETEERLRASESKFK
                     GFFDSAPDAMIIVNDEGQIILSNTQTEKMFGYSEEELSGRMIEVLVPERFRHKHPGHR
                     GGYFANLRPREMGADLELFALHKNGSEFPVEISLNYHQTGKETIVLCAIRDITDRKNS
                     ERILRDSEERFQQMANHIQEIFWMTDAVSGEELYISPAAEAIWGHTPEMLMHNPGLFL
                     RSVLPEDRSNVTQTITDQRNGIKTEVEYRIKGSDGSIRWVWDRAFPIIDESGKVSVVV
                     GIAADISERKSSEKEVIRHLTELEALYENGLAVGRLLEPKDIGDRIISTFSKYLSWHH
                     VAIRLKDPDSDELRLITFNREGLNEEAASDVKLRLESSLSKVGQGLSGWVVQRGQSFR
                     SGSVHDHPQYFSTFPNIQSGLYMPLKIGDVSIGVISVESEEPNAFTEQDERLLATLAS
                     QAAVALENARLYEAVQQELSERVRAEEQLEQERNSLAQRVDSRTADLTRAMRELERAM
                     RVKDEFLANMSHELRTPLNAILGLSESLAEQTAGPLNEKQQKYITTISESGYHLLSLI
                     NDILDLAKIEAGQVTLDINKVDLNSVCQSSLRMIKQMAQKKNQVVNLDIDPDVSLIWA
                     DERRLKQMIVNLLSNAVKYTPKDGTLGLEVLGDRGENKVRVTVWDNGIGIKEQDLPLL
                     FQPFVQLDSGFAREFSGTGLGLALVAQMARLHGGRVSVVSTFGEGSRFTLVLPWEPML
                     VADTVERMKSTGTYPAVINASEHNRRTILLIEDTPEVVLVLTD"
BASE COUNT          853 a          668 c          823 g          704 t
ORIGIN      
        1 ttgggaggaa gatttctccc tggtaaaaaa acgagtagac attttgcaga aagagggagt
       61 aaacaatttc agagattatt tttcgaccca tccggaccaa gtggcagaac ttgcaaccct
      121 agtgcgcctc gtcgatgcaa accaggccgc catgaaatta tctggtgtag agtggaaaga
      181 tgatctgctg aagagcccaa gcaatccgat tagtaatgga gccaacctca actttcagga
      241 agaacttctg gggctgatga gccctcggat gggattcaag atggagagaa ttcaaaggaa
      301 tgcaaagggt gagcaattgc aggtccttgt cagtgggaga atcccatatg gctacgaagc
      361 ggactggtcc aaagtcattg tctccatcag cgacatcagt gatcgcaagc gtatggaaga
      421 ttcgctgcgt gacagagaac agcgtttgaa cgcgatcatt gaaaatgaac cggaatgcat
      481 caagctgctg gattcagacg gaactgttct gcagatgaat gcagccggac tggaaatgat
      541 cgaagcggat ggtccggaag atgtattagg caaaccgatt tattccatcg tcacagaaga
      601 attccgtcag gaatttcaaa atttaacgga aaaagtatta agaggtgaaa gcggaaaact
      661 tcaattcgag atcgttgggc tgaaagggac acaccgctgg ctggataccc atgccgttcc
      721 catgcgaaac gcagatggag aagtgttcgc gctcctgggg atcacccgca acatcaccga
      781 atataaagaa acggaagaga ggttgcgagc cagtgaatcg aaattcaaag gtttttttga
      841 ctcagctccc gatgcaatga tcattgtgaa tgatgaaggg caaattattt tatccaacac
      901 acaaaccgag aaaatgttcg gctattctga agaggaattg tccggaagga tgatcgaagt
      961 tctggtgccg gagcggttcc gccataagca tcctgggcat cgcggtgggt attttgcgaa
     1021 tctacgccca cgcgaaatgg gcgcggatct ggagcttttt gcactgcaca aaaatggcag
     1081 tgaattcccg gttgagatta gtctcaatta tcaccagacc ggcaaagaaa caattgtact
     1141 gtgcgcaatc cgcgatatca cggatcggaa aaactctgag cggatattac gcgatagcga
     1201 agaacgtttt caacaaatgg caaaccatat tcaagagata ttctggatga ccgatgccgt
     1261 ctcaggtgaa gaactctata taagtccggc tgccgaagcg atttgggggc acacgcctga
     1321 gatgctcatg cacaatccgg gtctatttct tcgaagcgtc ctgcccgagg atcgctccaa
     1381 cgttacacag acgatcaccg atcaacgcaa tggcattaag accgaggtgg agtatcggat
     1441 taaaggctcc gatgggtcaa ttcgttgggt gtgggatcgt gcatttccaa tcatagatga
     1501 gtctggcaaa gtctctgttg tagtagggat tgccgctgac attagtgaac gcaagagttc
     1561 cgagaaagag gtcattcgtc atcttacgga gctggaggcc ctttacgaaa atggattggc
     1621 ggtcgggcgt ctcctggagc ctaaggatat cggcgaccgg atcatctcga ctttttcaaa
     1681 gtatctttcc tggcaccatg ttgcaattcg cttgaaagat cccgatagtg atgaattaag
     1741 actcatcacc tttaataggg agggtctgaa tgaggaggca gcaagcgatg tgaagcttcg
     1801 cctcgaatct tcgctcagca aagtgggtca gggcttaagc ggttgggttg tgcagcgcgg
     1861 gcagtctttc cgttctggca gcgtccacga tcaccctcaa tatttttcaa catttcccaa
     1921 tatccagtct gggctatata tgccgcttaa aatcggtgat gtcagcatcg gagtcatcag
     1981 tgtcgagagc gaagaaccaa atgcgtttac cgagcaggat gagcgcttac tggcgaccct
     2041 cgcaagtcag gcggcagttg ccctggagaa tgcgcgcctc tacgaagcgg ttcaacagga
     2101 attatccgaa cgagtgcggg cagaggaaca attggaacag gaacgaaact cattggcgca
     2161 gcgggtggac agtcgtacag ccgatctcac tcgggcaatg cgcgaactcg agcgggcaat
     2221 gcgtgtgaag gacgaatttc ttgcaaatat gagtcacgaa ctacgcactc cattgaacgc
     2281 gatcctgggt ctatctgaaa gtctggcgga acagactgcc ggtccgctca atgagaagca
     2341 acaaaaatat attacgacga tcagtgaaag tggttaccat ttgctctcgt tgatcaatga
     2401 cattcttgat cttgccaaaa tcgaagcggg tcaggtcacg ctggatatca ataaggtcga
     2461 tctaaattca gtttgccagt caagcctgcg tatgatcaag caaatggcgc agaaaaagaa
     2521 tcaggtcgtg aaccttgata tcgatcctga tgtcagctta atttgggcag atgagcgccg
     2581 gctcaaacaa atgatcgtaa atttattgag caatgcagtc aaatacacgc caaaagatgg
     2641 aacgctgggt ctggaggttc tgggggatcg aggcgaaaac aaagtcaggg tcacagtctg
     2701 ggacaacggc attggaatca aggaacagga tttgcccctt ctatttcaac cctttgtgca
     2761 gttggactca ggcttcgcta gagaattttc tggtacaggt ctcgggctgg ctttagttgc
     2821 acaaatggcc cgtctgcatg gcggaagagt gagtgttgtc agtacttttg gcgaaggcag
     2881 tcgcttcacg ctggttttac cctgggaacc catgctggtg gcagacaccg tggagagaat
     2941 gaaaagcact ggtacatacc cggcagtgat aaacgccagt gaacataatc gtcgaacgat
     3001 tctcctgatc gaagatacac ccgaagtagt gttagtgctc actgatta
//
LOCUS       DASTJP010000228         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_424831, whole genome shotgun sequence.
ACCESSION   DASTJP010000228 DASTJP010000000
VERSION     DASTJP010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424831"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFQ23_06935"
     CDS             complement(<1..330)
                     /locus_tag="VFQ23_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9906356.1"
                     /translation="MHFVSRIEPASAKTPEVYLQNSTGFRRATYVDRAMGSVHMGTGI
                     CYLDAHGVIQPHMHSFEESFYILEGSVILQINEQAYELGPGQFGLIGTGVPHGWRTVG
                     DTTARWLE"
     gene            complement(442..1143)
                     /locus_tag="VFQ23_06940"
     CDS             complement(442..1143)
                     /locus_tag="VFQ23_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9906357.1"
                     /translation="MTNGTVVVVGGTCQLGADLAKHYADKGNRVVITSRDAGRAQATA
                     KEIGGDTSGIALDLANPHGIADALAGLDDVLHLVLVALYRDDNKVREYNIDDAIQLVT
                     LKLVGYTECVHVLCSKMSDDSSILMYGGLARNQPYPGSTTITTVNGGVATLINSLAVE
                     LAPIRVNAIHPGQVGDSVNWATKPSAVLENLKSRTALGRLVTIEDVTHAAVFLLENRG
                     ITGVNLRVDGGRMMK"
     gene            complement(1136..2047)
                     /locus_tag="VFQ23_06945"
     CDS             complement(1136..2047)
                     /locus_tag="VFQ23_06945"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9906358.1"
                     /translation="MPSRHIVRTADQEDFNVPKPFENISNGFRRWCIVDGPNGSVHQE
                     FSICELDPGGTIGAHVHTFEEQIYVLEGELICETGDGTYSLDPGDYGVIHVSAAHSFR
                     NDSDKRVRWAEMLAPQPRLDGEGDIYPVAALPKVEPIKVDARDPRTRSFGHISPENME
                     SGKQTQDMLAVSASMRTALLVYSGITVKMMVDSDLGAQLATMFMVQYEPAGVAGAHDH
                     PLEETYLILEGEVYGWFDGVKYHLKPGDCAWAGVGCVHEFRNATDGIVRWLETQAPQP
                     PRRHSYRFRRDWKYLEEKLSKGDHHHD"
     gene            complement(2061..>2905)
                     /locus_tag="VFQ23_06950"
     CDS             complement(2061..>2905)
                     /locus_tag="VFQ23_06950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9906359.1"
                     /translation="HNKPIALTRILASPKARRLAQERGLDLKSISGSGPDGAVLAIDL
                     PAESPSPVGRGIRGEGEISRIWRVMADRLSEAWRTIPHFYLMREVNASRFVAWRETAQ
                     KNSAEKITYTDLMVKLTAAALKRYPRVNASWQNDNIVLNSDINIGLAVAVEDGLLVPV
                     IHQADGMNLSQLASRRAEIVRRAKDNKLSLDDLNGGTFTISNLGMYGVDAFNAIVNPP
                     QAAILAVSRIADRVVAVNGQPAVQPMMTLSLSCDHRVVDGARGAEFLQALADLIEEPL
                     QLLN"
BASE COUNT          702 a          807 c          709 g          687 t
ORIGIN      
        1 ttccagccag cgagccgtcg tgtcgcccac agtcctccaa ccatgaggga cacctgtgcc
       61 aatcaatcca aactggcccg gtccaagttc atatgcctgt tcattgattt ggagaatgac
      121 gcttccctcc aaaatgtaaa agctttcttc gaacgaatgc atgtgaggct gaatgacgcc
      181 gtgcgcgtcc aaatagcaga tgccggtgcc catatgcaca gaacccatcg cacgatccac
      241 atacgtggcg cggcgaaacc ccgtactgtt ttgtagataa acttccgggg tcttcgcaga
      301 agcaggttca atcctggata caaagtgcat gggtatacct ttagttatgt catgctgagc
      361 aaagcgaagc atctggactg tgcccagtct gagacccttc ggtcgcaata accgctccct
      421 ccgggtgaca cagtgaacgt gtcatttcat catgcgtccg ccgtcaacgc gcaggttgac
      481 accggtgatg ccgcgattct ctaatagaaa aacagcggca tgagtcacat cttcaatcgt
      541 gacgagtcgc cccagtgcgg tgcgcgattt caagttttcc agcactgcgc tgggttttgt
      601 ggcccagttg actgaatcgc caacctgacc cggatggatc gcgttcacgc ggatcggcgc
      661 gagttccact gccagtgaat tgatcaaggt tgcaacaccg ccattgacgg tagtaatcgt
      721 ggtcgagccg ggatagggct gattgcgcgc caaccctcca tacatcagaa tggaggaatc
      781 atcgctcatt ttcgagcaaa gcacgtgtac gcattcggta tagcccacca gcttcaacgt
      841 gacgagttga atcgcatcgt cgatgttgta ttcacgaact ttgttatcgt cacgatataa
      901 ggctactaac acaagatgaa gtacatcatc caatcccgcc aatgcgtctg caatgccatg
      961 cgggtttgcc agatccaacg caatcccact tgtatctccg ccgatttcct tggcggtcgc
     1021 ctgtgcacgt cccgcatcgc ggctggtgat tacaactcga tttcccttat ctgcgtagtg
     1081 tttggccaaa tcggcgccaa gctgacacgt gccacccaca acaacgacgg ttccattagt
     1141 catggtggtg atctcctttg ctcaattttt cttcaagata tttccaatca cggcggaagc
     1201 gatacgaatg gcggcgcggc ggttgaggcg cctgtgtctc cagccagcgc acaatcccat
     1261 ctgtggcgtt gcggaattca tgcacacaac ctacacctgc ccaggcacaa tcgccaggct
     1321 tgagatgata tttcacacca tcaaaccagc catagacttc gccttccagg atcaaatagg
     1381 tctcttccag cgggtgatca tgagcgcctg ccacaccggc aggttcatac tgtaccatga
     1441 acatggtcgc tagttgcgca ccgagatcgc tatccaccat catcttgacc gtgatcccgc
     1501 tgtagactaa cagagcagtg cgcatactcg ccgatactgc cagcatatcc tgtgtttgtt
     1561 tgccagattc catattctca ggcgagatat gaccaaaaga ccgtgtgcgc ggatcacgcg
     1621 catcgacctt aatcggttca accttcggga gagcggcgac cggataaata tcaccctctc
     1681 catccaggcg gggttgcggg gcgagcattt ctgcccagcg cacgcgtttg tcactgtcat
     1741 tgcggaagga atgtgcggcc gaaacatgaa tcacaccata atcaccaggg tccagcgaat
     1801 atgtaccatc accggtttca cagatcaatt caccttcaag gacatagatc tgttcttcaa
     1861 acgtatggac gtgcgcccca atggttccac cagggtcaag ttcacaaatg ctgaattcct
     1921 gatgaacaga accattcggt ccatccacga tgcaccaacg acgaaaacca ttgctgatat
     1981 tttcaaacgg tttcggtacg ttaaagtctt cctggtcggc tgtgcgaacg atatgtcgtg
     2041 agggcattct gtctcctgaa ttaattcaac agttgcaaag gttcttcgat cagatcagcc
     2101 agtgcctgca aaaactctgc gccacgcgca ccatctacca cacggtgatc acaggataag
     2161 ctcagggtca tcatgggttg gacagcaggt tgtccattga ccgccactac acgatctgca
     2221 atgcgactca cggcaaggat cgcggcttgc ggcggattga cgatcgcgtt gaaggcatcc
     2281 acgccataca tccctaaatt actgatcgta aacgtgcctc cattcaaatc atccaatgag
     2341 agcttattat cctttgctct tcgtacgatt tctgcgcgtc gtgaagcgag ctgcgaaaga
     2401 ttcatcccat cagcctgatg gatgaccgga acgagaagtc catcttctac ggcaacagcg
     2461 agcccaatgt tgatatctga gttgagaacg atattgtcgt tttgccagga ggcattaact
     2521 cgcggatatc ttttcaaagc tgctgccgtg agcttcacca tgagatcggt gtatgtaatt
     2581 ttttctgcag aattcttttg agctgtttca cgccaggcaa cgaaccgaga tgcattcacc
     2641 tcacgcatca aataaaaatg aggaatcgtc cgccatgcct ccgagagccg atctgccatg
     2701 acgcgccaga tgcgggaaat ctctccctca cccctgattc ctctcccgac aggagagggg
     2761 gactccgcag ggagatctat agctagaact gcgccatcag ggccggaacc ggagatagat
     2821 ttgaggtcaa gcccgcgttc ctgagcaagt ctccttgcct tgggagatgc gagaattcga
     2881 gtaagggcaa tgggtttgtt gtgat
//
LOCUS       DASTJP010000229         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1483889, whole genome shotgun sequence.
ACCESSION   DASTJP010000229 DASTJP010000000
VERSION     DASTJP010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483889"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VFQ23_06955"
     CDS             complement(<1..1452)
                     /locus_tag="VFQ23_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9906360.1"
                     /translation="MIQSRSLRYDEQINRLESLTPILLAMHGAAMLLTLGGNPGSWVL
                     VIITALLGATVAIQSWTQKKLAALRAAGLMGISLALLFSTEDSNRFFLPWFFAIVSIY
                     PIVLNPPFRVLLPFVMAGIYLTMLLFPPREQSIVAVWAHSFLLLYIGLLTGKLGSILN
                     EQAANRDALIQEASDGIFITDLDGNFIEINQAGCSMFGCTRDEILRRNIRDVIILEAG
                     EEPLQLAELLAGHIIRTERLLRRKDGTQFPAELSGRRITGDRIQGIVRDITERRRDEA
                     AQQQLINWQKRLIETSPDGIVENDLNGKILNMNSRFAELFGFESAAEILSSGLSTFDL
                     LAQEEKDAVPEKAQPVFDVGVVRNVEMKVVRRDGTVFEAEFSTTMMDGEGNDSRTLIT
                     FIRDITERKQTEAALQAAEAKYRALVERLPAVVYTSDLGTTGAWHYVSPQIESLLGFT
                     PEEWIADPGLWYQRVHVDDRERQETLEEQALESG"
     gene            complement(1838..2161)
                     /locus_tag="VFQ23_06960"
     CDS             complement(1838..2161)
                     /locus_tag="VFQ23_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9906361.1"
                     /translation="MAEDKDANSLTIRDYLETHGYEIVVARDGLEAVQKAQLIHPKLI
                     LMDIQMPVMDGLQAIRNIRADLHFTTTPIIVLTALAMPGDQARCLQAGTSDYMSKLGQ
                     FETII"
     gene            complement(2148..2468)
                     /locus_tag="VFQ23_06965"
     CDS             complement(2148..2468)
                     /locus_tag="VFQ23_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906362.1"
                     /translation="MRYCDERLTLARDEAGKLDYFPQVVSVAEICKSGISFITSQALK
                     KSVTVTYDEQISQVKIYADPRLLKQILVNLRASAIKFTPEKGEVGLQINADLEKDLIQ
                     FGRR"
BASE COUNT          690 a          692 c          623 g          689 t
ORIGIN      
        1 gccgctctcg agggcctgct cctccagggt ctcctgacgt tcacgatcat ccacgtgtac
       61 tcgctggtac cagagacctg ggtcagctat ccactcttcc ggagtgaaac ctaaaagcga
      121 ttcgatttgc gggctgacgt aatgccaggc acccgtggtg cccaggtccg aagtgtacac
      181 aactgctggc aatcgttcca ccagggcccg gtatttcgct tccgccgcct gcaatgccgc
      241 ctctgtctgc ttgcgttcgg taatgtcccg aatgaaggtg atcagagtcc gagagtcgtt
      301 accctctcca tccatcatcg tggtgctgaa ttccgcttca aatacggttc catctcgtct
      361 gaccactttc atttccacgt tgcgaaccac acctacatca aagactggct gagccttttc
      421 cggaaccgca tccttttctt cctgggcgag cagatcgaag gtgctgagtc cgctggataa
      481 aatctctgcg gcgctctcaa aaccgaacag ttcagcaaat cttgaattca tattgaggat
      541 cttgccgttc aaatcgttct cgacgattcc atccggactc gtctcgatga gccttttttg
      601 ccagttgatg agttgttgtt gtgccgcttc atcccttctg cgctcagtga tatcgcgtac
      661 aatgccttga atgcgatcgc cagtgatcct ccggccactg agttcagccg gaaattgtgt
      721 gccatccttg cggcgtaata acctctccgt gcgaatgatg tgacccgcca gcaattcagc
      781 cagctgcaac ggctcctccc cggcctccag gatgatcacg tcacgaatat ttcttctgag
      841 tatttcatcc cgggtgcatc caaacataga acagccggcc tggttgatct caataaagtt
      901 cccatccaga tcggtgatga atatcccgtc agaagcttcc tggatcagtg catctcgatt
      961 tgcagcctgc tcatttaaga tcgagccaag tttgccggtg agcagaccga tatacagcag
     1021 gagaaaggag tgcgcccaaa ccgcgacaat tgattgttct cgtggtggaa atagaagcat
     1081 cgtcagataa atgccggcca ttacaaaggg cagcaagaca cgaaatggcg gattgaggac
     1141 gatcgggtaa atggagacga tagcaaagaa ccagggtaga aaaaaacgat tcgagtcctc
     1201 cgtgctaaat agcaatgcca ggctgatacc catcagtccc gcggcccgaa gtgcggctag
     1261 ttttttttgt gtccaactct gaatcgccac tgtagccccc agtaatgccg tgatgataac
     1321 caacacccag ctaccggggt tcccccccag cgtgagcagc atcgccgcgc catgcatagc
     1381 cagtaagatt ggtgtgagag actccagccg gtttatctgt tcgtcatatc gaagagatcg
     1441 actctgaatc ataggattac ctcagatgcc aaaaagagca gctttatgaa taagtctgat
     1501 catagaggat tgcagattgg agctggtaat agcttacaac aaacgccaat ttcattccgc
     1561 ccagggcagt ttggaggtct gtgtcggcag gcgggtaatg atcctcaaca agggcattgc
     1621 aattggataa accatttttc aacctacaac aaggcaaata agaccttcaa aaccagaatt
     1681 agtataacac tgctgggttg gcagatgaaa gtacgattct tccccgggtt tcgatgtcat
     1741 tgcgtaccta tagcccctgg gttaggtagc aatatctatt gccatcgttt tgaacccatc
     1801 tcgagagggc aacaactata acaaggttgt gatcttttca aataatagtt tcaaactgac
     1861 ccagcttgct catataatca ctcgtaccgg cttgaagaca acgcgcctgg tcgccgggca
     1921 tagccagggc agtgagaaca atgatcggtg tcgttgtgaa gtgcaagtcg gcccgtatgt
     1981 ttcgaatcgc ttgtaagcca tccataaccg gcatttgaat atccatcaga ataagtttcg
     2041 ggtggatcaa ctgcgctttc tgtaccgctt ccaacccatc gcgtgcgacg acaatttcat
     2101 acccgtgtgt ctccaggtaa tcgcggatag ttaaagagtt ggcatcctta tcttctgcca
     2161 aactgaatca ggtctttttc caaatcagca ttgatctgta aacccacctc ccctttttca
     2221 ggtgtgaatt tgatggcact agcccgcaga ttgacgagga tctgtttaag cagccttggg
     2281 tcagcatata ttttgacctg ggagatctgc tcgtcataag taactgtaac ggatttcttt
     2341 aaggcctgtg atgtaataaa tgaaatacct gacttgcaaa tttcagcgac agaaacaacc
     2401 tgagggaaat aatcaagctt gcccgcttcg tcgcgggcaa gggtcaagcg ctcatcgcaa
     2461 taccgcactc ggtttcatcg tctgtaggtg gaagattgaa gctggtgggc ataaaactgg
     2521 agaaaactaa tatcactcag ccagatttgt aaaacccata ttttctctca cttcaaattg
     2581 atttatgcaa ttgctcctga aatctagcat ataatattgt catctagaaa cctcaaaaat
     2641 ctagtcaagg agaaatcata atgtcggttc aaaaatctct cctgatcacc tctg
//
LOCUS       DASTJP010000230         8918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1296508, whole genome shotgun sequence.
ACCESSION   DASTJP010000230 DASTJP010000000
VERSION     DASTJP010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296508"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFQ23_06970"
     CDS             <1..685
                     /locus_tag="VFQ23_06970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HET9906363.1"
                     /translation="DATADLAFALMLSVGRRLVEGQQYVREGQWKTWSPTLLLGIEMK
                     EATLGLIGFGRIGKAVARRARGFDMRVIYYDPTETETHSDLNATRVDFETLLKESDFI
                     SLHTPLTEKTRHIIDSAALSKMKPTAVLINTARGPIVDPDALFEALKHHRIFGAGLDV
                     TDPEPIPLDSPLLTLDNIVIVPHLGSASITARNNMSWLAARNLIAGLKGEPLPHCVNP
                     QVYSQTKIS"
     gene            783..1937
                     /locus_tag="VFQ23_06975"
     CDS             783..1937
                     /locus_tag="VFQ23_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET9906364.1"
                     /translation="MSVHMRSPFHIILITLIALGVFGPQAVSAKAKDNQVTLASYELI
                     GTVNIPTGAQFETTEIGGLSSITYDAARNVYYAISDDQGTIDPVRYYTLDIDVSDGQL
                     DAGDITFLEVTTMLDASGAPYVPGSLDPEGLTLAHEGSLYFSSEGFATRVPPVNPFIN
                     RLNLNGRHNRSLVIPEKFLPSSTQGVRNNLGFESLNITPDQKTLVTASEGALVQDGPA
                     ANLDQSSLARILQYRLSSGQPGNEYVYVVNPVAETPVPSTQFRVNGLVELLPLDNLGT
                     MLALERSFSVGAEGLGNTIWLYEIQTQGATDVSGIFSLVNQTFTPVAKRLILNVETDL
                     GIEPDNIEGMAFGPALPDGRIPLILVSDNNFAATQSTQFIVLAVELESTN"
     gene            complement(2066..7504)
                     /locus_tag="VFQ23_06980"
     CDS             complement(2066..7504)
                     /locus_tag="VFQ23_06980"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HET9906365.1"
                     /translation="MNKLLFKMFSSITILALVLMALPVQYALAATTIAQWTFESPNNP
                     AGATAATYPNAIAPATGTGNAGGVHASSSTVWSTPAGNGSTLSFSSNNWAIGDYYQFS
                     TSTSTYTDIQVSWHQTRSSTGPSDFKLAYSTDGTNFTDFDTYTIPANTWSTSGSPVVG
                     SIFTRDLSAVTSIDNQANVYFRLISTSAAGGTGGTNRVDNFTVSGEPIVVGDNAPTVT
                     GTSPTNGATNVPVNTNITVNFSEAVDVVAGAITVECPAGTPVASNTAVDNLSSVVIDP
                     ASDLPVSTTCAINVAASGVTDEDTNDPPDNMAANFNSSFTTLTPVVSSVIINELDSDN
                     PSTDTLEFVELYDGGVGNTSLTGLVLVFYNGSNDLSYAAFDLDGFSTNANGYFLLGNT
                     GVSPAPSITFADNFLQNGADAVALYGANGADFPNNTPVSTSNLIDAIVYDTADADDAG
                     LLVLLNPSEPQVNEDTGANGTMNSNQRCPNGSGGARNTSSYSQFGPTPGGFNFCANDA
                     APEVSSTNPTNGATDVAVNTNITVNFSEAVDVAAGTITVECPAGTVVASNAVVDNLSS
                     VVIDPASDLPFGTTCNINVSASGVTDDDANDPPNNMAANFSSSFTTLTPVVSTVINEV
                     LASTAGTDVEYIELYGGPGASLAGLSLIYVESNNTGAGTIDFRYNFAPTDTLGLNGFY
                     LIGVTSLLQATYGVTPNADIPTNSLENSSATVALVETSSLSGTSVGGSEVVLDAVGIT
                     DATASTFFFSAPVVGPDGTFFPAGVRRVTDGVDTDTTADWVISDFNLGPANTPTAGTT
                     ADVAPTVTATNPANGATAVPVGSDITVTFSESVDVAAGAISVECPVGALVASNDAASD
                     VTNVTINPASDLPANTACVISINPSGVTDNDGSPDALTGTTSFSFTTGAGTATTLISA
                     IQTNDDTSAAGTFTVEAIVVGDYQTQGSGQLRGFFLQEEDTDADADPLTSEGIFVFCS
                     ACPAPVAVGDKVRITGSSSEFFGMSQLSATTAGSVNLLSSGNPLPTPASVQLPVPGVP
                     SGDLAAATSAINAYFEPFEGMLVTFPDTLSVSEYFELARFGQVILNEGGRPHTFTAVN
                     TPTALGYTNHQINLAARTIILDDTDNRENRPVDTPNTNYYHPVPGLSTSNFFRGGDTI
                     TNLTGVLHWSFAGLTGTDAWRLRPVTEAYSYAFTPVNTRPALPSVDGSLKVASFNVLN
                     YFLTIDTSNTCAPTQNQDCRGADSVQELQRQRTKMLAALSAINADVFGFMEMENTTGV
                     EPLADIVAGLPGYDFIDTGVVGTDAIRVGIIYKSSTVTPVGDFAILDSSVDPNFIDTR
                     NRPALAQTFEENATGGRFTVVVNHLKSKGSGCGSGDDDTTTGQGNCNGTRTLAAQALA
                     NWLASDPTGSGDSDVLIIGDLNSYAKEDPIVALQNSGYTDLVALFGGPNAYGYVFDGQ
                     LGYLDHALSNASMTPQVADVAEWHINADEIPLFDYNDDQRTADEATFEEESDTLPLYE
                     PNEFRTSDHDPVIVGLDLDVPDNVAPETTIDSNPADPSNSNSASFSFSGTDDVTVPGN
                     LNFECQLDGGGFTACTSPQSYTSLSDGSHTFQVRAIDEAGNVDSTPASFTWTIDTVAP
                     TISVAAGGVCSASGGTMNLTVTDTNSVNLSGSSSNTTAVPNANIVFGGSGSNRTVGIT
                     AVAGSTVRTATVTITATDAAGNTSSTTITVTVGTNANNTLNGTSGADLILGLDGNDTL
                     NGLAGNDLLCGGDKNDTLNGGDNDDTLDGGDKNDTLNGGNGNDGLYGGNDNDLLTGGA
                     DADSFSGGPGNDINTDFNAGQGDTNDGT"
     gene            complement(7577..>8918)
                     /locus_tag="VFQ23_06985"
     CDS             complement(7577..>8918)
                     /locus_tag="VFQ23_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906366.1"
                     /translation="NAFNAGAVVSGNSAGAAVESLNMIGGYTGSNGPENGFQQGSVDL
                     WLPGGSDPNRRGLSFGMTTAVLDQHEFQRGRIARLINTSFTTGLLGIGADADTGMVIR
                     DEDTLTDVTGETAALVIDLQTYNANGQYAGPTNSLAIHGVTTHLIPPGSFGYDLIHRR
                     PVVDGHSRPAPSLAGRSFDALHLPSGHGPLILAGDLSGDRSGSAAQRFVTLSGGGNAR
                     LVVLAFGYAKNTDAQANAKAFASALQPFVTNPVQWFVVDSKVNQTAVQNAISNATGVL
                     VTAPDQSRVLSAFAAAPNITNAIRNAWASGKVLLADNAAAAALGAATSTDATPTSAGL
                     ESDSMGDFLFSGVSIQPGLNWIPGVAIEPRMVMDRHWGRAYNHLYRNQSLLGLGVDVN
                     TAIEFTSAGATVWGQNTVVVFDGRFASFSLGTNDALSARYVLLDTYVEGDSIAP"
BASE COUNT         2122 a         2318 c         2341 g         2137 t
ORIGIN      
        1 cgatgcaaca gccgatttgg cttttgcctt gatgctaagt gttggtcgcc gcctggtgga
       61 aggccagcag tatgtgcgtg agggtcaatg gaagacctgg agtcccacgc tcctgctcgg
      121 aatcgaaatg aaggaagcta cgcttggact gatcggcttt ggacgaatcg gcaaagcagt
      181 ggcgcgccga gccaggggat ttgacatgcg agtgatctat tatgacccta cagaaacaga
      241 aactcattcg gacctcaatg caacgagagt cgattttgaa acccttctaa aagagtccga
      301 ttttatttca cttcatacac cgctgacaga gaagacacgc cacatcattg actccgctgc
      361 cctttcgaag atgaagccta cagcagtgct gatcaatact gcgcgtggac cgatcgttga
      421 tcccgatgca ctctttgagg cattgaaaca ccatcgcatc tttggcgccg ggctggatgt
      481 cactgacccc gagcctattc cattagatag ccctctactg actctcgata atatcgtcat
      541 cgtgccgcat ctgggaagcg ccagtattac cgcgcgcaat aatatgtcct ggttggcggc
      601 cagaaatctg atcgcaggcc taaaaggcga gccattgccg cattgtgtca acccgcaggt
      661 gtattcgcaa acaaaaattt cgtgaccttt tgtgctagaa tttggtttcc acgttcacgc
      721 acatcgccat atatccatcg cttctgtttc aagaccaaac ctactttttc ggaggtgaca
      781 agatgtctgt tcatatgcgt tccccttttc atatcatcct gatcacgttg atcgcgcttg
      841 gtgtttttgg cccgcaagct gttagtgcga aagcgaaaga taaccaggta acactagcca
      901 gctatgagtt gatcggcacc gtcaacattc ccacgggcgc tcaatttgaa accacggaaa
      961 tcggagggct ctctagcatc acttatgatg cggcacgcaa tgtttattat gccatttccg
     1021 acgaccaggg cacaatcgat ccggttcgct attacacgct ggatattgat gtgtctgatg
     1081 gacaactgga cgcgggcgat atcacttttc tggaagtgac aaccatgctg gacgcatccg
     1141 gcgctccgta tgtgcctggg tctctggacc cggagggatt aacactggcg catgaaggct
     1201 cgctatactt ttcctctgaa gggttcgcca ctcgtgttcc tccggtcaac cccttcatta
     1261 accggttgaa tttgaacggc cggcacaatc ggtctctggt tattccggaa aagttccttc
     1321 cgagcagcac acagggagtg cgcaacaatc ttggtttcga aagcctgaat atcacgccag
     1381 atcagaaaac tctggtgacc gcttcggaag gcgcgttagt tcaggatggt ccggccgcga
     1441 atctggacca gagcagctta gctcgaattc tgcaataccg actaagctcc gggcagcccg
     1501 gtaatgaata tgtttacgtg gtgaatccgg tggctgaaac gcccgttccg tcgacgcaat
     1561 tccgcgtcaa cggactggtg gaactgttgc ctctcgataa tctgggtacg atgctggccc
     1621 tggagcgttc gttttccgtg ggtgccgaag gcttaggcaa cacgatctgg ctctatgaga
     1681 tacagactca gggagcaaca gatgtatctg gtatattctc cctggttaac cagaccttta
     1741 cccctgtggc aaagcgcctg atcctgaacg tggaaaccga cctgggaatc gagcccgata
     1801 acatcgaggg gatggcgttc gggccggcac ttccggatgg ccgtataccc ctgatcctcg
     1861 tgagcgataa taacttcgcg gctacccaat ccactcaatt tatcgttctg gctgtggaat
     1921 tggaatcaac gaattgatgt ggagatagca gtaacaaagc atatcgtaag attgtcgaat
     1981 tgaaatacca atcgattttg tacaacaaag aaggctggcg aatgccagcc ttctttgttt
     2041 gattgattct gaatttgaat cgacgctacg tcccgtcgtt cgtatctcct tgcccggcgt
     2101 tgaagtcggt atttatgtcg ttgccaggac caccactgaa ggagtcagcg tcggcgccac
     2161 cggtcaaaag gtcattatcg ttgccgccgt atagaccgtc attgccattg ccaccattca
     2221 gcgtatcgtt cttatcaccg ccatccagtg tgtcatcatt gtcaccacca ttcaaggtgt
     2281 cgttcttgtc gccgccacaa agaagatcat taccggctaa accgttgaga gtatcgttgc
     2341 cgtcaagacc gagaatcaga tctgcaccag acgtaccgtt gagcgtgttg ttggcattcg
     2401 tgccgactgt cactgtaatt gtcgtcgagg atgtattccc agcggcatct gtcgcggtaa
     2461 tggtcaccgt tgctgtgcgg actgttgacc ctgccacagc agtgatccca accgtgcgat
     2521 tcgatccact gccgccgaac acaatgttgg catttggcac cgcagtggtg ttggacgaac
     2581 taccactcaa attcactgaa ttggtatctg tcacggtgag gttcatcgtg ccgcccgagg
     2641 ctgaacatac accaccggct gccacagaaa tggtcggagc gactgtatca atcgtccagg
     2701 tgaaggaagc cggggttgag tcaacatttc ctgcctcatc aatggcgcgc acctggaaag
     2761 tgtggctgcc atcgctcaag ctggtgtagc tctgcgggct cgtacaggca gtaaatccgc
     2821 caccatctag ctgacactcg aagttaaggt tgcctggcac cgttacatcg tcagttccac
     2881 taaaggagaa tgacgcggaa ttgctattgc ttggatcagc cgggttgcta tcaattgtag
     2941 tttccggcgc aacattatct ggtacatcca gatctaatcc aacgattaca ggatcatgat
     3001 ctgaagtacg gaattcattc ggttcataga gtgggagggt atcagactct tcttcaaaag
     3061 tagcttcatc tgctgtgcgt tgatcatcgt tatagtcaaa aagcgggatc tcgtcagcgt
     3121 tgatatgcca ttctgctacg tccgccacct ggggggtcat gctagcgttg gaaagagcgt
     3181 ggtcgaggta tccaagctga ccgtcgaaca cataaccata ggcattcgga ccaccaaaca
     3241 acgcaaccaa atcagtgtag cctgaattct gcagggcaac aatcggatct tccttggcat
     3301 aggaattcaa gtcaccaata atcaagacat ccgagtcgcc acttccagtt gggtcagaag
     3361 ccagccaatt agccagtgcc tgggcagcaa gagtgcgggt accattacaa ttgccttgtc
     3421 cggttgttgt atcgtcatcg ccggagccac agcctgagcc tttgcttttg agatggttaa
     3481 ctacaacagt gaagcgtccg ccggttgcat tctcttcaaa agtctgagcc agagccgggc
     3541 ggttgcgggt atcgatgaag tttggatcaa cactgctgtc cagaatggca aagtcaccaa
     3601 ccggtgtgac cgtggaactc ttgtagataa tcccaacgcg tatggcatca gtgccgacca
     3661 cacctgtatc gatgaaatcg tatcctggca atcccgccac aatatcagcc agcggctcga
     3721 cgccagtcgt gttttccatt tccatgaagc cgaagacatc tgcattaata gcagagagtg
     3781 ctgccagcat cttggtgcgc tggcgttgta gctcctgaac actgtcggca ccgcggcagt
     3841 cctgattttg agtgggcgcg caggtgttgc ttgtatcaat agtcaggaag tagttcaaaa
     3901 cattgaaact agcaaccttc aaactcccat caacactagg tagagccggg cgcgtattta
     3961 caggtgtaaa agcgtaactg tatgcttcgg tcacgggacg aaggcgccag gcatctgtgc
     4021 cagtaagacc agcgaatgac cagtggagca caccggtgag gttggttatc gtatcgcctc
     4081 cacggaagaa gttacttgtg ctgagtcccg gcacggggtg gtagtagttt gtgtttggag
     4141 tatcaactgg gcggttctcg cggttatccg tgtcatccag gataatcgtg cgggctgcta
     4201 agttgatctg atggttagtg tagcccagtg ctgttggggt gttcacagcc gtgaaggtgt
     4261 gtgggcgtcc gccttcgttc aggattacct gcccaaagcg agcaagttcg aaatattcac
     4321 tcacagatag ggtatctggg aaggttacca gcatgccttc aaagggttcg aagtaagcgt
     4381 tgatagctga ggtggcagct gctagatcac cgcttggcac accgggtaca ggcagttgga
     4441 cgcttgctgg ggtgggcaaa ggattgccag agctaagtaa gttgacactg ccagcagtag
     4501 ttgcgctcaa ttggctcatc ccaaaaaact cactagaaga gccagtaata cgtactttat
     4561 ctcctacagc taccggcgct ggacaggcac tacagaatac aaagattcct tcggaagtga
     4621 gcggatccgc atcggcatcg gtatcctctt cttgcaggaa gaaaccgcgt agttggcctg
     4681 atccctgagt ctggtagtca cctaccacaa tagcctcgac cgtgaacgta ccggctgcgg
     4741 acgtgtcatc attagtctgg atagcactaa tcagcgtggt cgctgtacca gcacctgtgg
     4801 taaagctaaa actggttgtg cctgtcaaag catcaggtga accatcgttg tcagtcacac
     4861 cacttggatt aatagagatc acacaggcag tgtttgccgg cagatcactt gcaggattaa
     4921 ttgtcacgtt tgtgacgtca ctggcagcat cgttgctcgc taccaaggcc cctaccgggc
     4981 actcaacgct gatcgcgcct gcagccacat ctacgctttc gctgaatgtg acagtgatgt
     5041 cgctaccgac cggaacggcg gttgccccat tcgcaggatt cgttgcggtc actgtaggag
     5101 cgacgtcggc tgttgtacca gcggtcggtg tgtttgccgg accaaggttg aagtcactga
     5161 tgacccagtc tgcagttgta tcagtatcca caccatcagt tacacgccgg acaccagcag
     5221 ggaagaaggt accatccggg ccaacgactg gagcgctaaa gaagaacgtg ctggcggtcg
     5281 catccgtgat gccaaccgcg tccagcacga cttcactacc gccaacgctg gtgccgctaa
     5341 gactagatgt ctcaactaaa gccaccgttg cgctgctatt ttccaggcta ttagtgggaa
     5401 tgtcagcgtt cggggtcacg ccataagtag cctgcagcaa ggatgtaacg ccaatgaggt
     5461 agaagccatt cagaccaagc gtgtctgttg gggcgaagtt atagcggaag tcgatcgttc
     5521 cggctccagt attgttgctt tcgacataaa tcaggctcag acctgccaaa gaagcgccgg
     5581 gtccaccata aagctcgatg tattccacat ctgtaccagc agtacttgct agtacttcat
     5641 tgatcacggt gctgacaacc ggcgttagcg tcgtaaagct ggaactaaaa ttagcagcca
     5701 tgttgtttgg tgggtcgttg gcatcatcat ccgttacgcc actggctgat acattgatgt
     5761 tacaagtggt accaaaaggc aaatcgcttg ccggatcaat gacaacactg gataggttat
     5821 caactaccgc attgctggcg accactgtac cagcaggaca ctcaacagtg attgtgcctg
     5881 cagctacatc gactgcctcg ctgaagttga ctgtgatgtt tgtattcaca gcgacatcag
     5941 ttgcgccatt tgttggattg gtggaactta cttccggcgc agcatcattc gcgcagaagt
     6001 taaaccctcc aggggttgga ccaaactgcg agtagctgct ggtattacgt gcgcctcctg
     6061 aaccattcgg acagcgttga ttggagttca tggtgccgtt ggcgccagtg tcttcgttga
     6121 cctgaggttc actgggattt agcagcacaa gcaatccagc atcatctgca tccgccgtgt
     6181 catagacgat ggcgtcaatc agattgctgg tagatacagg cgtgttattc ggaaagtctg
     6241 ccccattggc accataaagc gcaacggcat ccgcgccatt ctgaaggaag ttatcggcga
     6301 aggtaatgct cggagcgggg ctgacacctg tattgcccag aaggaagtac ccattagcat
     6361 tggtgctaaa accatccaaa tcaaaggcag catacgacaa gtcattgctt ccattgtaga
     6421 agactaacac cagccctgta agacttgtat tcccaactcc accatcgtaa agttcgacaa
     6481 attccaatgt gtcggtgctc gggttatccg aatcgagttc gttgataatc actgagctaa
     6541 cgacgggagt cagagtggta aagctggagt tgaaattagc agccatgtta tctggcggat
     6601 cattggtatc ttcatcggtt acgccactgg ctgcaacatt gatagcgcaa gttgtactga
     6661 ctggcagatc gctcgccggg tcaatcacaa cactggaaag gttatccact gcagtgttgc
     6721 ttgcgacggg ggtaccagct gggcactcga ctgtaatcgc gcccgcaacc acatccacgg
     6781 cttcactaaa gttgacagtg atattcgtat tcacagggac attcgtggcg ccattcgtcg
     6841 gagaagtgcc agtaactgtt ggtgcattat cgccaacaac aatcggctca ccactcaccg
     6901 tgaaattgtc gactcgattc gttccgccag ttccgccggc tgcactcgtg ctgataagcc
     6961 ggaaataaac attcgcttga ttgtcaatgc ttgtgactgc agagagatcg cgggtgaaga
     7021 tggatcccac cacaggcgag ccagaggtac tccaagtgtt ggcgggaata gtataagtgt
     7081 caaagtcagt gaaatttgtt ccatctgtgc tgtacgcaag cttgaagtcg ctggggcccg
     7141 tactgcttct agtctggtgc caactcactt gaatgtcggt ataggtcgag gtactggtac
     7201 tgaactgata ataatcacca attgcccagt tgttcgagct gaacgacaag gttgaaccgt
     7261 ttccagctgg tgttgaccat accgtgctgc tacttgcgtg aacacctcca gcattaccag
     7321 ttcctgttgc aggggcaata gcattgggat acgtcgctgc tgtagcacca gcggggttgt
     7381 tcgggctctc aaaggtccat tgggcaatgg tggtggcagc cagtgcgtat tgcactggca
     7441 acgccatcag caccaaagcc aggattgtga ttgatgaaaa catcttaaac aaaagtttat
     7501 tcataggttt ctcctcggtt agagtttata tagcgaatcg ccattgagtc aattgattgg
     7561 atagtgttga tttgatttat ggtgcaatcg aatctccttc tacatacgta tctaataaga
     7621 catatcttgc actgagtgca tcatttgtcc ccaaactaaa acttgcgaat cttccgtcaa
     7681 aaacgacgac cgtattctgt ccccagaccg tggcaccagc ggaggtgaat tctatcgcag
     7741 tgttcacgtc cacgccaaga cccagcaggg attgattacg atagagatga ttgtaggcgc
     7801 gcccccagtg acgatccatc accatgcgcg gctcgatggc cactcccgga atccaattca
     7861 agccgggttg aatggagacg ccgctgaaca ggaagtcacc cattgagtcg ctttccagcc
     7921 cggctgaagt gggcgtcgcg tccgtgctgg ttgccgcgcc cagcgcagcc gccgctgcat
     7981 tatcggccag taagaccttt ccactagccc atgcattgcg tatggcgttt gtaatattcg
     8041 gcgcagctgc gaaggcgctc aggacacgcg attgatccgg cgcggtgacc agaacacccg
     8101 tggcattact aatcgcattt tggacagccg tctgattgac tttggagtca acgacaaacc
     8161 actggacggg attggtcacg aagggttgta aggcagaagc aaacgctttc gcattggctt
     8221 gcgcatcggt attcttagca tatccaaaag ccaatacaac cagacgagca ttgccgcccc
     8281 cacttaaagt aacaaagcgc tgcgccgcag aaccagagcg atcaccactg agatcgccag
     8341 ccaggattaa cggtccgtgc cccgagggga gatgaagcgc gtcgaaactg cgacccgcca
     8401 acgacggcgc gggacgtgaa tgtccatcca ccacaggacg acgatggatg aggtcatatc
     8461 caaatgagcc gggtgggatc aggtgcgtcg tcactccatg aattgcgaga ctgtttgtgg
     8521 gacccgcgta ctgcccgttc gcgttgtagg tttgcagatc gatcacaagc gcggcagtct
     8581 cgcccgtgac atcggtgaga gtgtcttcat cgcgaatcac catgcctgta tctgcatccg
     8641 ctccaatacc cagcaagccc gtcgtaaagc tggtattgat cagccgggcg atgcgaccac
     8701 gctggaactc atgttgatca agcacggcag tggtcattcc aaaactcaag ccgcgccggt
     8761 tcgggtctga gccaccgggt agccatagat caacactacc ctgctggaat ccgttttcgg
     8821 gtccgttgct gccggtgtat cccccgatca tattaagcga ttcgaccgcc gcgcccgcgc
     8881 tgttgccact cactaccgcg ccggcattga aggcgttc
//
LOCUS       DASTJP010000231         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_729180, whole genome shotgun sequence.
ACCESSION   DASTJP010000231 DASTJP010000000
VERSION     DASTJP010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729180"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFQ23_06990"
     CDS             <1..681
                     /locus_tag="VFQ23_06990"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET9906367.1"
                     /translation="TFAVPHGGGGPGVGPVGVAAHLADFLPDPQVGIIEDGDDNTAPL
                     YGFIKPPKSIGRMKAFHGHFGGGFVRSFTYIAMHGPDGLKDISQYAVLNANYLLARVR
                     DVYHVPFDRICMHEFVAEGQFEGSSVRALDISKRLMDYNFHPPTNYFPLIVHEALMIE
                     PTETENKDSLDAFAEALIKIAEEAKTNPEIVKSAPNTTQFGRMDEVKAARELVLCCWL
                     PEGYDTNA"
     gene            complement(749..1459)
                     /locus_tag="VFQ23_06995"
     CDS             complement(749..1459)
                     /locus_tag="VFQ23_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906368.1"
                     /translation="MPTIPTSTSTKASTGTPEPTSTHTAISSRTPSPTITPDLAATQQ
                     YVDFLALVQSYHDAGQILTPDGEYVKLEDYEHSLANKLSYEWTETGVTAENFIVRADF
                     EWENAVNTANISGCGFVFRSQSNGDHYLIILDAASGVKLASSTDRGTYSMGSPQNGDQ
                     KVSDFGAGPYQANFTLIVNEFKTYVYINDIYQGEYELLDYRITESGVLGSAVLSATFD
                     GFGTRCRMTNVRAWIIEP"
     gene            1971..2483
                     /locus_tag="VFQ23_07000"
     CDS             1971..2483
                     /locus_tag="VFQ23_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HET9906369.1"
                     /translation="MTTQESGSAEIGPPLHIRKLHSLVGDWDVDFETRGAPNESFVVQ
                     RISSRIVSILGGAFLQEELSITTSSGNQVELLGLLGYDRYREIYRFAWLDDKYAIFDV
                     HEGNWQGDALVVNNLRSGTTFRFGHQDYFSRMTWHDITVDEFRVASDLSSDGGKTWFT
                     QARSRYIRKK"
BASE COUNT          714 a          645 c          647 g          713 t
ORIGIN      
        1 acctttgctg tcccacatgg cggtggtgga ccgggtgtag gccctgtcgg tgtcgccgca
       61 catcttgcgg attttctgcc agatccgcag gtcggaatca tcgaggacgg tgatgacaac
      121 accgcgcctt tgtacggctt cattaaaccg cccaaatcca tcggacgcat gaaggccttc
      181 catgggcatt tcggcggtgg cttcgtccgc tcctttactt acattgccat gcatggaccg
      241 gacggactca aggatatttc acaatatgct gtgttgaatg cgaattatct gttggctcgc
      301 gtgcgcgatg tttatcacgt cccatttgac cgaatctgca tgcatgaatt tgttgcagaa
      361 ggacaattcg aaggttccag cgtccgcgcg ttggatatct ccaagcgcct catggactat
      421 aacttccacc cacccacaaa ctactttcct ctgattgtcc atgaagcgtt gatgatcgag
      481 ccgactgaaa ctgagaacaa agattcactc gacgctttcg ctgaggcgct gatcaagatc
      541 gctgaagaag ccaaaacaaa tcctgagatc gtgaagtccg cgccaaacac cacacagttt
      601 gggcgcatgg acgaggtcaa agccgcgaga gagttggtgt tgtgctgctg gctgcccgaa
      661 ggctatgata cgaatgctta atcagcaata gagtcaaaaa cgcgaaaact ccacagaatg
      721 tcaaaaaaga attttgtgga gttttatttt aaggctcaat aatccaggcc ctaacattcg
      781 tcatcctgca gcgggtgcca aatccatcga atgtcgccga gagaacagcg cttcccagaa
      841 ctcccgattc tgtaattctg tagtccagca gctcgtattc gccctggtaa atatcattga
      901 tatacacata ggtcttgaat tcattcacaa tcaaagtaaa gttcgcctga tacggacctg
      961 caccaaagtc cgaaactttt tggtccccat tttgaggtga acccatcgag tatgttcctc
     1021 gatctgtact gcttgctagt ttgacaccgc tggctgcgtc caggatgatg aggtagtggt
     1081 cgccgttcga ttgggagcgg aatacaaatc cgcaacccga tatattcgct gtattaactg
     1141 cattctccca ttcaaaatca gcgcgcacaa taaaattttc cgccgtgacg ccagtctcag
     1201 tccattcata ggaaagctta ttcgccaatg aatgctcgta atcctccagt ttcacatact
     1261 caccatccgg ggtcaaaatc tggccggcat cgtgatagct ttgtactaat gccagaaaat
     1321 ccacgtattg ttgtgtagca gccaggtctg gcgttattgt tggtgatgga gtcctggatg
     1381 atatcgctgt atgagttgaa gttggttctg gcgttccggt agatgctttt gttgatgtgg
     1441 aagtcggtat ggtaggcaca tctccgcaag atgatagaaa cagaacgaga gaaaaagtca
     1501 acattatatg agtctgatac agttttagtt tcatttttca cctttagagc aagcggaaca
     1561 acgtgtatag taccactatg cgcgtccatg tccgccagtt taagggagcc cttgtagtca
     1621 cggcagggaa ataggtggat gacgtggaat cccagattaa tcagatcata cgattcctgt
     1681 cagaatcggt ctaagagcgc ggttgccttg taacggagaa gagtaaactg agtgccagtc
     1741 gaatgcatgt agatgatcgt agactactgc ttctcccttt acccttccca ttaatcacgg
     1801 ttcctcggta tgatatgcaa caggagatat ccaagtgcca tcccttcaat gtataccttt
     1861 cggaaaaagc atatgcgagg cccagggacg ctgctaatca accaaggtgt ggcgattgat
     1921 agggaaactt attcgattcg acacaggtac ttacgatttg gacgttcata atgacaactc
     1981 aagaatccgg gtccgctgaa attggaccac ccttgcacat ccgaaaactt cattccctgg
     2041 tcggcgactg ggatgtggat ttcgaaacac gcggtgcgcc aaacgaatcc tttgtagtgc
     2101 aacgtatctc gtcccgaatc gtctctatcc tgggtggtgc ttttttacag gaggaacttt
     2161 ccattacaac ctcaagtggt aaccaggtcg aactcctggg tttattgggc tatgacaggt
     2221 atcgggaaat ctaccgattc gcctggctcg acgataaata tgctattttt gatgtgcatg
     2281 agggcaactg gcaaggcgat gcacttgtgg tgaacaattt acgctctggt acaacgtttc
     2341 gatttggtca ccaggattat ttcagccgaa tgacgtggca tgacatcact gtcgatgaat
     2401 ttagagttgc atcggatcta tcttcagatg gtggaaagac ctggttcact caagccagaa
     2461 gtcggtatat ccgcaagaag taatttgcga caaggtatta tcggctcact tcatgcgtcc
     2521 aacattaaca tctaacagta aaatcaactg gaaatctcag acgcttagta ttttcacaaa
     2581 tccattgtat gactgaaatc tacgaagatg ctttcaagcg cgcagaggcc atcagataca
     2641 agccaggcac gccaaactca gacggcttta ctgctgatta ctgtagcgtt tatcaatcga
     2701 acagaggcga taacatatg
//
LOCUS       DASTJP010000232         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_461053, whole genome shotgun sequence.
ACCESSION   DASTJP010000232 DASTJP010000000
VERSION     DASTJP010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461053"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFQ23_07005"
     CDS             complement(<1..261)
                     /locus_tag="VFQ23_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9906370.1"
                     /translation="MIRVAITDDNPKILSSLRLLLTCSKEMQLVSETRNGQEAVDCAK
                     RLHPDVLVMDVHMPVLDGFAAVKKIQDVAESTCVILMSLDTDR"
     gene            511..1929
                     /locus_tag="VFQ23_07010"
     CDS             511..1929
                     /locus_tag="VFQ23_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9906371.1"
                     /translation="MEHTVSVMDIALVFILVLLNAFFVASEFALVSVRRTRIDQLAAE
                     GNGAAEVVKRAVRNLDRYIAATQVGITIASLLLGGFGEHALEPVLSVLFVWFPEMWSG
                     ITRAGLVAGLAYFIMTVLHVIIGELMPKSIALQRPEATALWIGRPMSFFALIFSPLIW
                     LLNGIGNFLLRRLGFHAAEGHSQVHSPEELDMLFTEAHKGGEINQTEFEILHRVVRFS
                     DTTARTIMIPRLEIEALPTTIARGDLTDFLQSRPHTRIPIYQDSLDNIVGIVNSKDLE
                     HLNYRELSQELEKLRAIVVSQNNGQPSPDNYHTSAEKTLNLTPLVFEATFVPETIRID
                     QLLAQFRNRQQQIAIVVDEYGGTAGLVTLADLLEEVFGDLPDEADETEPDIVKRPDGS
                     YQIAGRVSIDEVNELFGFGFPSDEANTMAGLVINALDRTASVGDEVEINGVRIRVDKV
                     DRFRITTLGVFFPEQQPPERTG"
BASE COUNT          552 a          508 c          510 g          556 t
ORIGIN      
        1 acgatccgta tccagagaca tcaaaatcac gcacgtagat tctgctacgt cctgtatttt
       61 cttcacggct gcgaagccgt ctaacacggg catatgcaca tccatcacca gcacatccgg
      121 atggagtcgt ttcgcacaat ccacagcctc ttgtccgttt ctggtttcag aaacgagctg
      181 catctcctta gaacatgtta gaagcaaacg aagtgaactt aggatttttg gattgtcgtc
      241 cgtaattgcg acacgtatca tcagaccatt cctttctaaa ggtaagtcta aatactgttt
      301 tgacatgtgt ctatcgggcg atttccccgt cagcattggt aaattccccc tggttttata
      361 cgaaaccaat acgggagggc tggtatacag ggatggggtg tcccccatag aaggatcaaa
      421 acctatggat tatattattt agtggtagat tcattcctac taccgtcgat tcaaattgaa
      481 attctgattt ccaggctaag aaacaaataa atggaacata ctgtctccgt gatggatatc
      541 gccctggtat tcatcctcgt cttactgaac gctttttttg tggcctctga atttgcactc
      601 gtgtcggtgc gtcgaactcg catcgatcag ctcgctgccg aagggaatgg cgctgcagag
      661 gtggtgaaaa gagcggtgag aaatctcgac cgatacatcg cagccaccca ggttggcatc
      721 accatcgctt cgctcttgtt aggtgggttt ggtgaacatg ctttggagcc agtattatcg
      781 gtgcttttcg tctggttccc agagatgtgg tcagggatta cgcgcgcggg actggtcgcg
      841 ggcctggctt acttcatcat gaccgtcctg catgtcatta ttggtgagct aatgccaaaa
      901 tccatcgcgc tgcaacgacc tgaagcaacc gcgctttgga tcggtcgacc catgtcattc
      961 tttgcgttga tcttctcacc gttgatctgg ttgctaaatg ggatcggcaa ttttctcttg
     1021 cgtcggttag gctttcacgc ggcagagggt cacagtcagg ttcattcccc ggaagaactc
     1081 gatatgttgt ttacagaggc ccacaaaggt ggcgaaatca accaaactga gtttgagatc
     1141 ttacatcgcg tcgtccggtt ttcggatact accgcacgca cgattatgat cccgcgactg
     1201 gaaatcgagg cactcccaac aacgattgca cgcggcgacc tcaccgattt tttgcaaagc
     1261 cgtccgcaca cgcgcatccc gatctatcag gattctttgg acaatatcgt tggcattgtc
     1321 aactctaaag atttagaaca tcttaattat cgtgagcttt cgcaagagct ggaaaaatta
     1381 agggcaatcg ttgtcagcca aaataatggt caaccatcac cagataatta ccatacgagc
     1441 gcagagaaga ccctcaacct gacaccattg gtatttgaag caacgttcgt acctgaaacg
     1501 atccggatcg accagttgct ggcacagttc agaaatcgcc agcagcagat cgccattgtc
     1561 gtggatgaat acggaggcac cgcggggctc gtcacgttgg cagatttact ggaagaggtt
     1621 tttggtgacc ttccggatga agccgatgaa acagaaccag atatcgtaaa gcgccccgat
     1681 ggcagttatc aaatcgcggg acgggtaagt atcgatgaag tgaatgaact gtttggattt
     1741 ggttttccgt ctgatgaagc caatacaatg gctgggcttg tcatcaatgc cctggaccgc
     1801 acagcttccg tcggtgatga agtagaaatc aatggcgttc gtatacgcgt agataaagta
     1861 gaccgcttcc gtatcacgac acttggtgta ttctttccgg agcaacagcc tcctgaaaga
     1921 acaggctaac ttttcgctta tgcccatccc cggtaactgg aaaaagaatc aaacggagca
     1981 agactcgcac aactgggtct tgctccgttt atctagctcg aattgtgaat tgtattttat
     2041 ggaaaaggta gaatgcgcat caaataataa tcaaagtaaa ttagaagaaa aaacgcaagc
     2101 aagtaaggga tgccctctct tacctg
//
LOCUS       DASTJP010000233         6938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_187708, whole genome shotgun sequence.
ACCESSION   DASTJP010000233 DASTJP010000000
VERSION     DASTJP010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187708"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VFQ23_07015"
     CDS             complement(<1..1018)
                     /locus_tag="VFQ23_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9906372.1"
                     /translation="MKRNLESLVNQKFDVLVIGGGIHGAIAAWDAVLRGLSVGLIERG
                     DFGCGTSQNSLKIIHGGLRYLQDGNLSRIRTMARERTTWMRIAPHLIHPLPCLVPTTQ
                     NISRSRLSMGLALKTNDILSFDRNYQFDPEKDILDGMIVSQRELARILPGYDVSTSTG
                     GAVWYDAQIYNSERLLLEFILSAVDAGAVVANYVEATNFLHQEDRIIGVQARDLQTGQ
                     TFDIQSKLVINCAGAWADCLLEKAALRSEYVTSVAMNVIVNQVWSDISVGLTSQPVNG
                     KPPQVLFIVPWRNKSMIGTYHIPWGNVPHAFKLNEVIIQEFLDHINSAHPSLKLTLDD
                     VQHVN"
     gene            complement(1019..1786)
                     /locus_tag="VFQ23_07020"
     CDS             complement(1019..1786)
                     /locus_tag="VFQ23_07020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906373.1"
                     /translation="MNQAEMISRIATYWDEHIHDLAVTTFPVGTPEFFRQLDEYRYEK
                     LNYLPLRVDFPAYTGKSILEVGCGVGIDLVRFAGGGATVTGIDLSKTAINLARKNFVQ
                     NNLPADLRIMNGECMQFEDNTFDVVYAHGVLQYTADSSKMITEIHRVLRPGGEAIMMV
                     YNKNSWLNLMRNVTNVALEHEDAPVLKKFSLSELKHLLQPFQNYRIIPERFPVKTRLH
                     SGWKAQLFNTVFVGTFNSLPKTWTKAFGWHLMAFAVK"
     gene            complement(1783..3684)
                     /gene="asnB"
                     /locus_tag="VFQ23_07025"
     CDS             complement(1783..3684)
                     /gene="asnB"
                     /locus_tag="VFQ23_07025"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9906374.1"
                     /translation="MCGICGAVSFQPNSPIDRSTLLKMNSSLYHRGPDDEGYYADDQV
                     GLAMRRLSIIDLRSGQQPISNELGNIWAVYNGEIYNFQEVRAALEQRGHTFKTQTDTE
                     VILHAYEEYGDQCVTHFNGMFAIALWDAREHRLFLARDRLGIKPLYYWSDSEKLVFGS
                     ELKALLLHPDVPRLVDLAALDLFLTLEYIPAPRTIYKGISKLLPGHHLVVENRELKVT
                     QYWDVPYNPIHQSEAECGEILSGLIKESVRLHLISDVPLGAFLSGGIDSSTIVGFMSQ
                     SANVPAQTFSIGFEDNTYNELPYANAVAKYFGTKHHFEVLKSDHADLMEQLVAHFDEP
                     FADTSIFPTFLVSKLASRHVKVVLSGDGGDELFGGYDTYLADKLDQYYARLPRLFRQQ
                     VFPNVAEWLLPQPAKKGWINKVKRMVEGGSFDPSFQHARWMMFLNSSQKNSLYRSDLQ
                     TILDHQYTSDFFSDYFEKANSFDRLAQQQYVDVKSYLADDILTKVDRMSMAVSIEARV
                     PLLDYRIVEFALNLPTRMKLNGTRTKSILRQAVKNLVPQFVLEKPKEGFSIPMKRWLC
                     TSLKPMMLDLLSNDSLQKHDYFDRQVVARWIREHLDGRVNHSHRLWALMVFEMWRQNE
                     KTSAYGLHS"
     gene            complement(3686..4894)
                     /locus_tag="VFQ23_07030"
     CDS             complement(3686..4894)
                     /locus_tag="VFQ23_07030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9906375.1"
                     /translation="MKILMISPQPFFEPRGTPISVYQRLEALSALGHEIDLLTYHVGK
                     DVEFPNVKIHRVMNVKMIQHVRIGPSRAKILLDLLVFLKAIQLLLRKRYDVIHSHEEA
                     AFFSMFLAWIFRTSHLYDMHSVLSNQLGNFDFGNKPIFINSFRILEQMVLKTCAAVIT
                     VGTDLEKYVRSVGTNIRVVMIENIALQAYQTAVEPEDVQQLKAKLGLDGRLPIVYTGT
                     YEQYQGLTVALDCVELVAKQFPEVIFIFVGAKNQQAREWSQIARGRNLQEHVLFLDVV
                     PPAESMVYLAYAAVLISPRLDGTTTPLKIYSYLHANRPIVATNITAHTQVLNSETAIL
                     VEPDKTALADGILQVLQDPELADCIARNAHELAREKFSRQDYIAKVNQIYQSISVVRE
                     VEEPALSWRK"
     gene            complement(5159..6400)
                     /locus_tag="VFQ23_07035"
     CDS             complement(5159..6400)
                     /locus_tag="VFQ23_07035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9906376.1"
                     /translation="MSTAAAAKRMRIAYIVPYVPNLIRTRPYNLITQLSALGHEVTVF
                     TLGSNHADQIEAQRLKSHCHEVEYFDQPLWRSLWNSATALPSERPLQSVYSWQPAMAR
                     EIVKRASRNEFDIVQIEHLRGSRFGLYLKSRLPNLPVVWDSVDCISHLFQQAAKHSSS
                     FFGTFMTRFELGRTRKAEGHLACRFDHVLVTALSDKNALLELVPKGMTPSKLSVLPNG
                     VDLEYFRPNANVQREPETLVFSGKMSYHANISMAKYLVQEIMPRIWKVRPNVVLYIVG
                     KDPNPTIKKMMENPLIRVTGTVDDIRPFLWRATVSVVPLLYGAGIQNKILEAMATGTP
                     VVTTCQALSALQAQPGKDLFASDDPDGFSQAVLGLIADRDLQQQTGDAGSQYVRTFHS
                     WSSIASRLVNVYQQTLGNHSE"
     gene            complement(6485..>6938)
                     /locus_tag="VFQ23_07040"
     CDS             complement(6485..>6938)
                     /locus_tag="VFQ23_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HET9906377.1"
                     /translation="RSYQDKIHIVYPVHLNPNVRKTVYETLSDIPNISLIEPVDYEAL
                     VGLMSKAHFIMTDSGGIQEEAPSLHKPVLVLRETTERPEAIDAGAAKLVGTDPDKIYT
                     EAVRLIEDSENYYQMSHAINPYGDGQASQRIVQFLLEHPIEKVADLSK"
BASE COUNT         1882 a         1641 c         1540 g         1875 t
ORIGIN      
        1 agttgacatg ctggacgtca tccaaagtaa gcttgagtga ggggtgagcg ctattgatat
       61 gatcgagaaa ttcctgaatg atgacttcgt tcagtttgaa ggcgtgtggc acattccccc
      121 atgggatatg ataagtaccg atcatggatt tgttcctcca cggcacaata aagagaactt
      181 gtggaggctt tccatttacc ggttgactgg ttagtccgac tgagatatct gaccagacct
      241 gattcacaat cacattcatt gctacggatg tcacatattc agaccgaagc gcagctttct
      301 caagcagaca atccgcccag gctcctgcac agtttatcac tagcttcgat tgaatgtcga
      361 aagtctgccc cgtctgtaaa tctctggcct gtacaccgat gattcgatct tcctggtgta
      421 ggaaattggt ggcttcaaca taatttgcaa ccaccgcacc cgcgtccact gcagaaagaa
      481 tgaattccaa aagtaacctc tctgagttgt aaatctgggc gtcataccag accgcgccgc
      541 cggtcgatgt cgagacatca tatccgggca aaattcgggc cagttcacgt tgcgaaacga
      601 tcataccgtc cagaatatcc ttttcagggt caaactggta atttcgatca aaacttagaa
      661 tatcatttgt cttgagcgca agccccatgg ataagcggct acgggatata ttctgtgtcg
      721 taggtactaa gcacggcaga ggatggatta ggtgaggagc gatcctcatc caagttgtgc
      781 gttcgcgcgc catggtgcgg attctcgaaa ggttgccatc ttgaaggtac cgtagaccgc
      841 cgtgaataat cttgaggctg ttttgcgacg ttccacaccc aaaatctcca cgttcgatca
      901 agcccacaga taagcctcga agaactgcgt cccaagcggc aatcgcgcca tgtatccctc
      961 cgccgatcac caggacatca aatttctgat taaccaggct ttcaagattg cgtttcattt
     1021 atttaactgc aaatgccata agatgccagc cgaacgcttt ggtccacgtc ttcggcagcg
     1081 aattaaacgt tccaacaaac acagtgttaa aaagctgagc tttccaacct gaatgcagcc
     1141 tggtcttaac tggaaaacgc tcagggataa ttcgataatt ttgaaaaggt tgcagcagat
     1201 gtttcaattc actgagtgaa aattttttca ataccggagc atcctcatgt tcgagtgcca
     1261 cgttggtaac atttcgcatc agattaagcc aggaattctt gttataaacc atcataatag
     1321 cctccccacc tggtctcaag actctgtgga tttcagtgat catttttgaa gaatctgctg
     1381 tatattgcaa aacaccatgt gcatatacaa cgtcaaaagt gttgtcttcg aactgcatgc
     1441 attcgccatt cataatacgc agatcggcgg gcagattgtt ttgcacgaaa ttcttgcgcg
     1501 ccagatttat ggccgttttc gataggtcaa ttcctgttac cgtcgctcct ccgccagcaa
     1561 aacgaactag atcaattccg acgccacaac ctacctccag gatactcttt ccggtgtaag
     1621 ctgggaaatc aacccgcagc ggtaggtaat tcaatttctc atagcgatat tcatccagct
     1681 gtctgaaaaa ttctggcgta ccaactggaa atgtcgtgac ggcaagatcg tggatgtgtt
     1741 catcccagta ggtcgcaatc cgcgagatca tttctgcctg gttcatgaat gcaatccgta
     1801 cgccgaggtc ttttcgtttt ggcgccacat ctcgaagacc atcaatgccc ataatcggtg
     1861 actatgattg acgcgtccat cgagatgttc gcgaatccat cgcgctacaa cctggcgatc
     1921 gaagtaatca tgtttttgta gcgaatcatt cgaaagtaaa tcgagcatca tcggttttag
     1981 agaagtacac agccaccgct tcattgggat actaaatccc tccttcggct tttccagcac
     2041 aaactgcgga accaggtttt tgactgcctg ccgcagaatg gatttggtgc gtgttccatt
     2101 taatttcatt cgagttggca ggttaagtgc aaactccacg atgcgatagt caagtagtgg
     2161 cactcgggct tcaatggaca ccgccatact catcctatcc actttggtca gaatatcatc
     2221 tgccagatac gatttgacgt ccacatactg ttgttgtgcc aaacgatcaa agctattggc
     2281 tttttcaaag taatcgctaa aaaagtcgga cgtgtattga tgatccagta ttgtctgtaa
     2341 atcagaccga tataaggagt tcttctgaga agaattgagg aacatcatcc aacgagcgtg
     2401 ctgaaacgat gggtcgaatg agccaccttc caccattcgc ttcactttgt tgatccaccc
     2461 ctttttagct ggttgcggca gaagccactc agccacattg gggaaaacct gttgtctaaa
     2521 tagcctcggc aaacgtgcat aatattgatc tagtttgtca gcaaggtatg tatcatatcc
     2581 accaaacaat tcatcaccgc catcaccaga taaaacaacc tttacgtggc gactggctag
     2641 cttggaaacc aaaaaagtcg gaaagataga agtatcggca aaaggttcgt cgaaatgagc
     2701 aaccagttgc tccataagat cggcatgatc tgatttcagc acttcgaagt gatgtttagt
     2761 accaaaatat ttcgctactg cgtttgcata aggaagctca ttatatgtat tgtcctcgaa
     2821 tccgatcgag aacgtttgag ctggcacatt tgcgctctga ctcataaagc caacaatggt
     2881 actggagtcg atgcctccac tcagaaaagc tcccagaggt acatcgctaa ttaagtgaag
     2941 gcgcaccgac tcttttatca aacccgaaag aatctcccca cattctgcct cactttggtg
     3001 aattgggtta tatggcacat cccaatactg cgtgactttc aactctctat tttcaacaac
     3061 taagtgatgt ccgggcaaaa gcttggaaat gcctttgtaa atcgttcgtg gagccggaat
     3121 gtattctaat gtaaggaaaa gatcgagagc cgcaaggtca acgaggcgtg gcacatccgg
     3181 gtgaagtagt agtgctttga gttcagagcc aaacaccagt ttttccgaat ccgaccagta
     3241 atacagaggt ttaattccca accgatctct ggcgaggaaa agtcgatgct ctcgggcatc
     3301 ccacagggca atagcaaaca ttccattgaa atgtgtcaca cactgatccc cgtattcttc
     3361 ataggcatgg agtatgactt ccgtatcggt ttgagttttg aaggtgtgac cgcgttgttc
     3421 caaagcggcg cggacctctt gaaagttata aatttctccg ttatatacgg cccaaatgtt
     3481 gcctaactcg tttgaaatgg gctgttgccc ggaacgtagg tcaataatgc tgagacgccg
     3541 catggcaaga cccacttgat catccgcgta atagccttca tcgtcgggtc cgcgatgata
     3601 aagcgagcta ttcatcttca ataaggtcga acgatcaatg ggcgagttag gttgaaatga
     3661 aaccgcgccg caaataccac acatcttatt ttctccaaga tagagcaggc tcttcaactt
     3721 cacgaactac tgatattgac tggtaaatct ggttgacctt tgcgatgtaa tcttgtcgac
     3781 taaacttttc cctggccaat tcatgtgcat ttcttgctat acagtctgca agttctgggt
     3841 cttgcagtac ctgaagaatt ccatcggcaa gtgccgtttt gtctggttcc acaagaattg
     3901 cggtctccga gttcagtacc tgggtgtgag ccgtgatatt tgtagcgacg atcggtctgt
     3961 tcgcatgaag atagctgtag atttttagtg gggttgtagt gccatccagg cgtggagaaa
     4021 tcaatacagc ggcatacgca agatacacca tagattcagc gggaggaaca acatccagga
     4081 ataggacatg ctcttgcaag ttcctgcccc gagcgatctg actccactcc ctggcttgct
     4141 gatttttcgc accgacaaag ataaaaatta cttctggaaa ctgtttggca acgagttcca
     4201 cacaatcaag cgccacggtc agaccctggt actgctcgta ggtaccggta taaacaattg
     4261 gcaatctgcc atcgagtccc agtttggcct tgagttgctg cacgtcctct ggctcgacag
     4321 cagtttgata ggcttgcaag gcaatattct caatcattac gacccgaata ttggttccga
     4381 ccgatctaac atacttttcg agatcggttc caactgtgat tacagccgca catgttttaa
     4441 gcaccatctg ttccaggata cgaaagctat taataaaaat gggtttattc ccaaaatcga
     4501 aatttcccag ctgattggac aacacggagt gcatatcgta caaatgactc gtccggaaaa
     4561 tccatgccag aaacatacta aaaaaggcgg cctcctcatg agagtgaatg acatcataac
     4621 gctttcttaa cagaagttga atggctttta agaacactaa aagatctagc aagattttgg
     4681 ccctggatgg cccgatacga acatgctgga tcattttcac gttcatcact ctgtgaatct
     4741 ttacattcgg aaactctaca tcctttccga catggtaggt aagcaaatca atttcatgcc
     4801 caagtgcaga gagtgcttcc aggcgttgat agacgctgat tggagttcca cgaggttcga
     4861 agaagggttg cggagaaatc atcaatattt tcatgttata tgagtccctg aataacacgc
     4921 aaaagataaa gaaatttaag acaatctata tgaattcttg acaatccttt cacagattgt
     4981 caaaaggtca aagctactgc gtttgcagtt gtttgattag tttgcagtcg agtacaagtc
     5041 tcatattggg tatatagttg gaaaatacga aaacaagtgt atcgttgacc tgtgtccatc
     5101 tgaattcccc cacaaccatc ttcctaccat gttcccacaa acgaaatcta ttttctaatt
     5161 attcagaatg gttgccgagt gtttgttgat agacgttgac gagccgagat gcaatcgaag
     5221 accagctatg aaaggttctg acatattgcg aaccagcatc accagtttgt tgttgaaggt
     5281 ccctgtcagc aatcagtccc aatactgctt gtgaaaaccc atctgggtca tccgacgcaa
     5341 agaggtcttt tcccggttgc gcttgaagcg ccgaaagggc ctgacaagtt gtcacaacgg
     5401 gagttccggt cgccatcgct tccaatatct tgttttggat acctgctcca tatagaagcg
     5461 gtacaactga gactgttgcg cgccacagga aagggcggat gtcatcaact gttccagtca
     5521 ctcgaatcag aggattttcc atcattttct taattgtcgg gtttggatcc ttgccgacaa
     5581 tgtacagaac gacatttgga cgaactttcc aaattcgcgg catgatttcc tgcaccaaat
     5641 atttggccat cgaaatattc gcgtgataac tcatcttgcc actaaaaacg agtgtttcag
     5701 gttctcgttg aacgttggca ttaggacgaa agtactcaag gtcaacgccg ttaggcaata
     5761 cagaaagctt agaaggagtc attccttttg gaacgagctc aagcaaagca tttttgtcag
     5821 agagagccgt gacaagcacg tgatcaaagc ggcaagccag gtgtccttct gctttgcggg
     5881 tgcgaccaag ctcaaagcgc gtcataaacg taccaaagaa gctgctacta tgctttgccg
     5941 cctgttgaaa caaatggcta atacaatcga cactatccca tacaactggc agatttggca
     6001 atctggactt caagtaaagt ccaaagcgtg aacctcttaa gtgctcgatc tgtacgatgt
     6061 caaattcgtt tcgggaagca cgtttgacaa tttctcgagc catggcaggc tgccagctat
     6121 acacagattg aagtggtctt tctgacggaa gagctgttgc actattccac aacgaacgcc
     6181 aaagcggctg atcgaagtac tctacttcat gacagtggct cttaagccgt tgagcctcaa
     6241 tttgatccgc gtgatttgat ccaagggtga agactgtgac ttcatggccc agtgcgctca
     6301 gttgagtgat caggttgtac ggacgggttc gaattagatt tggaacatac gggacgatat
     6361 aagcgattcg cattctctta gccgcggcag ctgttgacaa gcattaactt tttcctgcaa
     6421 tattgttact acccagccta attgtttcta cggctgcact catagtgaac tcattaccat
     6481 tgccttactt tgaaagatcc gccacctttt caattggatg ctcaagcagg aattgaacga
     6541 tccgctggct tgcctgtcca tcaccatacg gatttattgc atgagacatt tgataataat
     6601 tctctgaatc ttcaatcaat cgtactgcct ctgtataaat cttatcagga tccgtaccga
     6661 ccaattttgc cgcaccagca tcaatcgctt ccggacgttc agtcgtttca cggagcacca
     6721 gtactggttt atgcaggctc ggagcttcct cctgaatccc accggagtcc gtcatgatga
     6781 aatgagcctt gctcatcaga cccaccaaag cttcataatc cactggctcg atgagagaaa
     6841 tattgggaat gtcacttagg gtttcatata ccgttttacg gacgtttggg tttagatgga
     6901 ctggatagac aatatgaatt ttgtcttgat agctccgt
//
LOCUS       DASTJP010000234         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_566899, whole genome shotgun sequence.
ACCESSION   DASTJP010000234 DASTJP010000000
VERSION     DASTJP010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566899"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(513..1016)
                     /locus_tag="VFQ23_07045"
     CDS             complement(513..1016)
                     /locus_tag="VFQ23_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906378.1"
                     /translation="MTENSSLPNATKPVWQTKRFLFLLSICAGILTIGAVGMYAWYFW
                     VNPCDPDRVERAATTLISQLNWYDSAYQFATSSSKTALTRPVQVLQEIAMDTNDLRVP
                     ACMQTTKAELVNYMRTVVRAFQAYMRSETDATVRGILDESSIHFNNFTTRLEAIRECA
                     PYCAPWD"
     gene            1190..>2414
                     /locus_tag="VFQ23_07050"
     CDS             1190..>2414
                     /locus_tag="VFQ23_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HET9906379.1"
                     /translation="MKHFSFRGFSLALRFLTILLCLIAFVPGAFPTALAQSNRTSLQP
                     AATTSLGFHISGRFVLDANGNNFIMRGINVPHNWYPEQTSQFQHVKAKGANTVRVVLS
                     SGQLWPKNSASDVANVINLCKTNKLVCVLEVHDTTGYGESAGAVSLAQAVAYWKEIKS
                     VLIGQEAYVIINIGNEPYGNINATGWINDTKNAIAEMRTAGFQHMLMVDGPDWGQDWE
                     FIMRDNAASVFNSDPNRNILFSIHMYGIFNTASKIQSYLSTFVNAGLPIAVGEFGHEH
                     SDGNPDEDSIFSITQANGIGYLGWMWSGGSGAGTLDMVTNFNPNQETWWGTRIIHGPN
                     GIAATSQEASVYSGISPTVYTISGNVETRGITLSTNDGTIETALSDANGNYFITVPAG
                     WSGTVTPSHECFTFPL"
BASE COUNT          661 a          550 c          599 g          604 t
ORIGIN      
        1 gctcaagagc acaaaccgtc ctttttgcgg gcgaatgtgt tgagaaagga gatcccagta
       61 ggctttgaat ggtagtttca taagtactct ctagatttca tcataattaa caaaaaacca
      121 cggtgcagaa gtgcaccgtg gcataaagac acagaaatcc tgaccgtact ctaacgatca
      181 aaaggcgcac tccgagaagg taaattgtgg ggtttgcgac gtgctatgat caacatggag
      241 tgcgttcctt tcttcagatt ttacttacaa ggcgagtgta catcaggaat gagatacttg
      301 tcaagtgaat atggcagtgg ctaagagagt gagtgaaaat ctacttagcc acagagagcc
      361 accagtaatc ctgaaagtcc aaccattgaa ccgtacgcgt tcaaatcggt gcggtccaag
      421 aaaacgttga acccgacgag ccgatacgcg agtgacattt catgggggct actcttattg
      481 gtgttggaaa atcagccttg atctacgaga tatcaatccc agggcgcgca atacggcgca
      541 cactccctga tcgcttcgag ccgcgtcgtg aagttattaa aatgaattga agattcatcc
      601 aaaattccgc ggacggtggc gtcagtttcc gatcgcatgt atgcctggaa ggcgcgaacc
      661 acggtacgca tgtaattgac caactcagcc tttgtggttt gcatacaagc tggcacgcgg
      721 agatcgttgg tatccatggc gatctcttgc aacacctgga cgggacgcgt cagtgccgtt
      781 ttcgatgacg acgttgcaaa ttgataggcc gagtcatacc aatttaactg actgatcaac
      841 gtcgtggcgg ccctctcgac tctatccgga tcgcaagggt tgacccagaa ataccaggca
      901 tacattccca ccgccccgat agttagtatc cctgcacata tgctcaagag aaatagaaaa
      961 cgctttgttt gccagactgg ttttgtagcg ttgggtaagg aggagttttc ggtcatattc
     1021 atcatgactt cgtgccaggt tgaaaattat aaagtactag ggttgtcaac gcgtagacga
     1081 atttctttcc ctcaactcta ggcgggatat gaccattgta ggttgtattt ggctttgcaa
     1141 ttcgctacaa taagaaaagc tcacgaagag gtcggagagg agaattaaga tgaagcactt
     1201 ttctttcaga gggttttctc ttgcgctcag gtttctaact atcttactat gccttatcgc
     1261 cttcgttccg ggagcatttc cgacagcgct cgctcaatca aatcgcacaa gcttgcaacc
     1321 tgccgcgaca acttctctag gattccatat tagtggcaga tttgtacttg acgcaaatgg
     1381 taacaacttc atcatgcgag gaattaatgt tcctcataat tggtatcccg agcaaacgag
     1441 ccaatttcaa catgtaaaag cgaagggggc gaataccgtt cgggtggttt taagcagtgg
     1501 gcaactctgg cccaaaaata gcgccagcga tgttgccaat gtaatcaacc tctgtaaaac
     1561 caataagctg gtttgtgttc tcgaggtcca tgacacaact ggctacggcg aatctgctgg
     1621 tgccgtcagc ctggcacagg cagtggccta ttggaaagag attaaaagcg tattgatcgg
     1681 tcaggaagcc tatgtgatca tcaatatcgg caatgagccc tacgggaata tcaatgcgac
     1741 aggctggatc aatgatacaa aaaacgccat cgctgaaatg agaaccgcgg gctttcagca
     1801 tatgctgatg gtggatggac cagactgggg tcaggattgg gaatttatca tgagagacaa
     1861 tgctgccagc gtcttcaata gtgaccccaa tagaaatatt ctttttagta ttcacatgta
     1921 tggtattttt aatacagcca gcaaaattca gagctatctt tcgacgttcg taaatgccgg
     1981 cttaccgatc gcagtggggg aattcgggca tgagcacagc gacgggaatc cagatgagga
     2041 ttcaatattt tccatcactc aagccaatgg gatcggatat ctgggctgga tgtggagtgg
     2101 tggcagtggc gctggcactc tagacatggt aacgaacttt aatccgaacc aggaaacctg
     2161 gtggggaacc aggatcatcc acggaccgaa tggaattgcc gcaacatctc aggaagcttc
     2221 agtttatagc ggaatttctc ccacggttta taccatctct ggaaatgtcg aaacacgtgg
     2281 gataacgctc agcacgaatg atggcacgat tgaaacagcc ctctcggatg caaacggcaa
     2341 ctatttcatt acggtcccgg ccggttggag cggaactgtc actccatcgc atgaatgctt
     2401 taccttccct cttc
//
LOCUS       DASTJP010000235         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_982367, whole genome shotgun sequence.
ACCESSION   DASTJP010000235 DASTJP010000000
VERSION     DASTJP010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982367"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..704
                     /locus_tag="VFQ23_07055"
     CDS             156..704
                     /locus_tag="VFQ23_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906380.1"
                     /translation="MATTSSSLWRHPDFLKYWAGESISLIGSLALPAAAQGPFVFAVA
                     MLMLGLFVSSSANPVYNITQVSLRQSIIPLRLQGRMNASMRFLVWGTIPLGSLAGGAL
                     GEVIGNSVHTAGWSAGRVACCALGILFACPKLAPGAFYRRRAGHVKLQSHPSSINKVG
                     RADGARNVISSANGEPAYEQTL"
     gene            688..1410
                     /locus_tag="VFQ23_07060"
     CDS             688..1410
                     /locus_tag="VFQ23_07060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906381.1"
                     /translation="MNKLYNELASWWPLLSPVADYAEEAAFFMQVLRKAGFRPNSSLL
                     ELGCGGGNNAFHLKPHFSQLTLTDLSPQMLQLSQMLNPESEHHQGDMRTIRLHRTFDV
                     VFVHDAIDYMLTLDDLRQALETASVHCKPEGMALFVPDHVRETFEPSTDHGGTDGEER
                     GLRYLEWTYDPDEQDSTYTTEYVYLLREGDQPAQVEHDRHTCGVFPRADWLRLLNEVG
                     FQAEVIPDPFERELFVARKKQT"
     gene            complement(1429..2043)
                     /locus_tag="VFQ23_07065"
     CDS             complement(1429..2043)
                     /locus_tag="VFQ23_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906382.1"
                     /translation="MPTDIPPLAYGTFYHIYNRGNNHENIFVEVRNYAYFLELWWKHV
                     SPIAETWAYCLLRNHFHIVVFIKNREDLITNLTGLEDLSGFNNKIKPPHQYFSNFFNA
                     YSRGLNIALQRTGAVFERPFKRIPIDTESYLLRLIIYIHQNPQKHKFVRDFRDWNYSS
                     FHELVSEVPSHLSRERVIQIFCSRADFIRIHQEIQPMENFEEED"
     gene            complement(2155..>2558)
                     /locus_tag="VFQ23_07070"
     CDS             complement(2155..>2558)
                     /locus_tag="VFQ23_07070"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET9906383.1"
                     /translation="PISRTREQLSWIYLHLPVTMGIAAVGASILNVVEHSGETLPREV
                     RMLLVVAIAAVLVSIAMILKSLKLPESLRRAYRTGAHVMFISGLLILFVGLTLLSAIP
                     LLLLLVVLMLAPVLYSVLVWIKVFDARELEM"
BASE COUNT          640 a          604 c          647 g          667 t
ORIGIN      
        1 cccatcatta aagacggatt gatttatgta atagatattc ggaacgggct gtacctcctc
       61 aggtatcatg gtcaatttga ggaagaagtg aacagcatcg gatttttgga aggcaattca
      121 aaccttggtg atgcaagatt attggatgtc ccttaatggc aaccacgagc tccagccttt
      181 ggcgtcatcc tgattttcta aagtattggg caggggaaag catctcgctg attggatcac
      241 tcgcgttacc tgctgccgcc cagggaccat ttgtttttgc cgtagcgatg ctcatgctgg
      301 ggctgttcgt cagcagcagt gcaaacccgg tctataatat cacgcaggtc agcctgagac
      361 agtcgatcat cccattgcgt ttgcagggac gcatgaacgc cagcatgcgc tttctggtct
      421 ggggaacaat tccgctgggg tcgctcgctg gcggtgcttt gggcgaagtc attggaaatt
      481 cagtccacac tgctggttgg agcgctgggc gggttgcttg ctgtgctctg ggtattcttt
      541 tcgcctgtcc gaagcttgct ccaggcgcct tctatcgaag aagagcaggg catgtaaaat
      601 tacagtccca tccttcatcc atcaataaag ttggcagagc ggatggggcc agaaatgtaa
      661 tatcatcagc gaatggagaa ccagcttatg aacaaacttt ataacgaact ggccagttgg
      721 tggccgttac tttctcccgt tgcggattat gccgaagaag ctgctttctt tatgcaagtg
      781 ttgcgcaagg caggctttcg tccgaattct tctttgctcg aactgggatg tggcggtggg
      841 aataacgcct ttcatctcaa accccatttc tctcaactga cactcaccga tctttcacct
      901 cagatgctgc agctcagcca gatgctcaac cccgagagtg aacaccatca aggcgatatg
      961 cggaccattc gcctccaccg cacgtttgac gtggtgtttg tccacgatgc catcgactat
     1021 atgttgacgc tcgatgattt gcgtcaagca cttgaaactg cttctgttca ctgcaagccc
     1081 gaggggatgg cgctctttgt gccagaccat gtgcgcgaga cctttgaacc ctccaccgac
     1141 cacggcggca cagacggcga ggaacgaggt ctacgctatc tggaatggac gtacgacccg
     1201 gacgagcagg acagcaccta tacgaccgaa tatgtttatt tgttgaggga aggggatcag
     1261 ccagcgcagg tagaacatga ccggcacacc tgtggcgtct tcccccgcgc cgactggcta
     1321 cgattactga acgaagttgg ctttcaagcc gaagtcattc ccgatccgtt tgagagggag
     1381 ttgtttgtgg cgcgtaaaaa gcagacctga caggtcagtt gaattaggtt agtcttcctc
     1441 ttcgaaattt tccataggct gaatttcctg atgaattctg ataaagtctg cgcggctgca
     1501 aaatatttgg atcactcttt ctctgctcag atggcttgga acctctgaaa cgagttcgtg
     1561 aaatgatgag taattccaat ctcggaaatc tctaacaaac ttatgttttt gcggattctg
     1621 atgaatgtat ataatcaatc gcaaaagata cgattcagtg tcgattggta ttcttttgaa
     1681 gggacgttca aataccgcac ctgttctttg taaggcgatg ttaagtccgc gtgaatacgc
     1741 attgaaaaag ttggagaaat attggtgagg aggttttatc ttattattaa aacctgacag
     1801 gtcttcaaga cctgtcaggt ttgttataag gtcttcccga ttttttataa aaacaacaat
     1861 atgaaaatga ttccttaata aacaatacgc ccacgtctcg gctatcggac tgacatgctt
     1921 ccaccatagt tcaaggaagt aggcgtagtt tcgtacctct acgaagatgt tttcgtgatt
     1981 gttcccgcgg ttgtagatgt gatagaaggt cccgtaggca agtggcggaa tatctgttgg
     2041 catattttct attttcacgt ctctagaccg gacgggtctt gctgaaggga ataaactctt
     2101 ttactgtaga taatcagaaa tcgatcggtc atctaaaccg acgcataacc caactcacat
     2161 ttccagttcc cttgcatcga acactttgat ccaaaccaga acgctataaa ggacaggcgc
     2221 cagcattagg actacgagta agagaagtag tgggatggct gaaagtaaag taagacccac
     2281 gaaaaggatc agcaaaccag agatgaacat cacatgagct cctgtgcggt atgcccgccg
     2341 caagctctcg gggagtttca ggcttttcag aatcatggca atgctgacga gcacggcggc
     2401 gatcgctacc accaatagca tacggacctc acgtggcagc gtttcgcctg agtgttccac
     2461 gacgtttaag atgctcgctc ccaccgctgc aatccccatg gtgacaggga gatgcaggta
     2521 gatccacgag agttgctcac gggtgcgcga gatgggtg
//
LOCUS       DASTJP010000236        18227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_235835, whole genome shotgun sequence.
ACCESSION   DASTJP010000236 DASTJP010000000
VERSION     DASTJP010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18227
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235835"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="VFQ23_07075"
     CDS             <1..1603
                     /locus_tag="VFQ23_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906384.1"
                     /translation="RLTSGDWDDTTPSPSPDGKKIAFTSNRNGFWDIYLLDLSTGDVT
                     QLTNTPEYEGAPTWSPDSSFLAFEAYLDENLEILVGPAENPLDGAVRLTTAPTSDHSP
                     SWAPGGRQIAFISNGEVILADLDRTDSGRFQNLSNTTNASESHPVWSPDGQRLAWASS
                     SQNIGRSGIYIWDRGRNLPATWIGEGDWPAWNTEGSQIISTLAAPNSTYLTIYSTDGK
                     LLQPLIPFPTTLRGLMWANLVMTESLPGRFQQAAEFTPAPLWAANGEPVSEGASGRWS
                     LVNLQGVQAPYPQMHDLADEAFDALRQRVQQEVGWDALASLENAFVPITTVLDPGFQE
                     DWLFTGRAFAINSLMTNAGWMAAVREDFGSQTYWRLYLRAQLQDGSLGEPLRDAPWDL
                     SARYNLDPKVYEQGGTYSEVPAGYWVDVTALASQYGWERVPALPNWRTFYRGARFTEF
                     TLTGGLDWYSAMLELYPPDVLLTPTRVLPPTTTPTRTPLPTWTPLPTRTPRPTWTPSP
                     TRSPTVTVTPSGTPPPSPTPPTVIP"
     gene            1600..2772
                     /locus_tag="VFQ23_07080"
     CDS             1600..2772
                     /locus_tag="VFQ23_07080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9906385.1"
                     /translation="MKKMIIRAERKDDSPVDEAVVSYNALRLRSAPLSAMVLLFFIII
                     LTACTPATSTETETGSTPTAVIAGEVATQPPVQIDVQPKPTIAPFRFFLPTPGAEPVL
                     GWRPPLYPIPWAVSAYDHFYFARPIAADNVNWPLAEYRYGGVFFAPNIVHTGVDIDAA
                     EGTPILAAGAGTVVSADWGLFSEVPGNISDPYGQAVVLKHDFGYQDRSLFTVYAHMSK
                     IIAVVGQHVETGDVLGLVGDTGVTTGPHLHFEVRVGGNTFYNTANPELWMAPPQGWGV
                     LVGRMTDEDGELLNLYPVEVRPMPSEVPLRRALTYAQGAANSDAFYQENLVLSDLPAG
                     LYKIAFEYKDKQLQFWADIYPGQITYFTFTDEDGFQVVPPPMPTLDFLPGTATVVP"
     gene            2909..4021
                     /gene="recA"
                     /locus_tag="VFQ23_07085"
     CDS             2909..4021
                     /gene="recA"
                     /locus_tag="VFQ23_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9906386.1"
                     /translation="MSPRRKSAAIEEQAPSRNSNVDGAKQAVLDKAVGDILKRYGDGS
                     IMRLGEAHGMVTEAFPTGSLSLDIALGVGGVPRGRITEIYGPESSGKTTLCQHIVAEA
                     QKMGGVAAFIDMEHALDPVYAARVGVDIDNLLVSQPDTGEQALEITETLVRSGGVDVI
                     VIDSVAALVPRAEIEGDMGDPSMGVQARLMSQALRKLSGAINQTKTAVIFTNQLRQKI
                     GVMFGNPETTTGGQALKFYASIRLDVRRIQAIKVGDEVIGNRTRVKVVKNKVAPPFRT
                     AEFDIMFNEGISKSGDVLDLATKFEVVQKRGAFFSYGDIRIGQGRENAKDYLRQNFDL
                     MNEIDTVIRQKALSGEIALPLDMAVEANGGAAEEEE"
     gene            4165..4734
                     /locus_tag="VFQ23_07090"
     CDS             4165..4734
                     /locus_tag="VFQ23_07090"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9906387.1"
                     /translation="MSSRYQALQHRVGLTGILLTALFTSACSPATTPTPFRPPTAQAP
                     LIEPTFIINPTQPIVVVQSSPLPTIIPTTRPEDCSSNLTFVSDLTIPDNTSITYGSTI
                     DKQWIVENSGSCHWGPNYRLRQIGGATLGAPEEVALYPARAGTQATIQILFTAPFTDG
                     EYESAWQAVDPQGIAFGDPIFVRIVAVAP"
     gene            4744..5115
                     /locus_tag="VFQ23_07095"
     CDS             4744..5115
                     /locus_tag="VFQ23_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906388.1"
                     /translation="MATQADFPVVFEKLKSILKPYAETLTVKTDSMDTYYLDGPYTEK
                     WKKELFFGSAQIKKNYVSFYLMPVYMYPDLLKGISPDLKKHMQGKSCFNFKKVEPELL
                     QELTELTEKGYARFKQEELLK"
     gene            5128..5601
                     /locus_tag="VFQ23_07100"
     CDS             5128..5601
                     /locus_tag="VFQ23_07100"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9906389.1"
                     /translation="MKFKLEIPIGKPRDEVWKAFDNIENMKKWQPSLISFEPISGTPG
                     QPGAVSKLTYEENGREFSLIEKITLRDEPHRFDGVYENNFANNIIRNRFIEHGTDQTL
                     WVTETEYKFKTLLMKIAGPLLKKNFVARTKRDMERFKDVAEGKPDQEMENIEKEP"
     gene            5598..6512
                     /locus_tag="VFQ23_07105"
     CDS             5598..6512
                     /locus_tag="VFQ23_07105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9906390.1"
                     /translation="MKLLKRILIGILIAVVALVLLLFGSIAIDAVASRGRIDQVANTV
                     IPGLNGGPDVRAYVARPQGEGPFPVVIMIHEFMGLNDSITGRADLLAESGYLVIAPDT
                     FRGSTTSWLPRAIYLVITTKAEAVNADLDSVYAWLETQEDVSTNRIGIAGFCYGGRTS
                     LAYSLHNPKLAATVIFYGAPETEVEVLKTLPGPVLGIFGGADTSIPVETVNAFESGLE
                     QAGIPHQITIYEGQPHAFVSDKEKIEAGGAPAEAWNEMLEFLEENLKNKASQNMDSAP
                     LVYKAPFTWRYYLALAFEHAFGEASHVH"
     gene            complement(6605..7618)
                     /locus_tag="VFQ23_07110"
     CDS             complement(6605..7618)
                     /locus_tag="VFQ23_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET9906391.1"
                     /translation="MKTFVYIDHFKGEVQPGSWEALGLAKSLGAATALIFGSGVDNVP
                     QSAFEYGADEILLADDPSLLDYRAETYAATLSALVSAQSPDLILFPTTTRTRELAAMS
                     ALDLNTNVITDATAVDVNGDSIVVTRPIYEAKLFEKVKSTGQPQFITLRGRAFQKPEQ
                     QEGKTGTVTKVDAKTDGLSTVEGYSVAEVGVSLNDAGVIVSGGRGVSNNPSLQPPAGL
                     DDKQAEIWRAQQGFKLVGDLASVLGGAVGASRAAVDAGYIPYSNQVGQTGKVVAPDLY
                     IASGISGAIQHLAGIRSAKMVVAINKDPDAPIFKVARYGVVGDLFQILPALTEAFKKK
                     LGK"
     gene            complement(7615..8385)
                     /locus_tag="VFQ23_07115"
     CDS             complement(7615..8385)
                     /locus_tag="VFQ23_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET9906392.1"
                     /translation="MKIVACIKQVPDSEAKVRAEGGQVSWGDAPLVINPFDEYAVEGA
                     LQQKEATSGTVTALCIGPESAKEALKHALAMGADDAILVSDPALNNLDTVGAARIIAA
                     AIQKIGSVDMVVFGRQTLDSGSGITPAQTARVMGWPMLGLAGQIKAEDGSVTVERVIE
                     EGRQIVKAKAPVVISVVQSIGEPRYPSFMGIRKASKAEIPVWSLNDLGVSAPESVVSR
                     IEVMNPPARDTSVEIITGESPAEIADKLADKILAEKVL"
     gene            complement(8937..9227)
                     /locus_tag="VFQ23_07120"
     CDS             complement(8937..9227)
                     /locus_tag="VFQ23_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906393.1"
                     /translation="MDIKPQHTKVDKTIQTLHGWVVLEDSNGFPRSESNLYCVSVSGK
                     VIWKAEKPDPNTLYSRVRLNEDGATISTYTIGSHACELELGTGKIVSKTSLQ"
     gene            complement(9238..9804)
                     /locus_tag="VFQ23_07125"
     CDS             complement(9238..9804)
                     /locus_tag="VFQ23_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9906394.1"
                     /translation="MLDFQPVREKKLTLQDLVIGLTKADLRRELNDMYDEVSRLIADC
                     TDAQVTFVPVDEKAADPYAVEGEETISWTLGHVIVHLTASMEESAAIASELARGVAFH
                     GRSRSEVPWREATSIEYCRARLEESRRMCLASLEMWPDEPYMENTYSPAEGAVPHTAV
                     SRFASGLRHASDHLEQLEEIVRQAEEAS"
     gene            complement(9808..11016)
                     /locus_tag="VFQ23_07130"
     CDS             complement(9808..11016)
                     /locus_tag="VFQ23_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9906395.1"
                     /translation="MNFSLSNEHQMAQKMVRDFAQKEIAPVIKEYDRKQEPIPFALKR
                     MGELGILGICFPVRYGGQGMDYISWGLACEELEAMDTSLRVAMSVHTGLCSMSLFQWG
                     TEEQKQKFLVPLASGKKIGCGAFTEPGMGSDVAAMSTSAKRDGDHYILNGEKMWISLA
                     SKADLALVTVKTNPSARKGSDGLSSFIVDLHSDGVKTGDLHGKLGVRAGATGWISFTD
                     VKVPVENRIGEEGEGFKITMSGFDHGRYTVASGATGIIRAALEASVNYAKGRKTFGKP
                     IAEHQLIQEKIAKMSQDYEIARLLYLQVGWLKNEGKRPTRETSFAKKFATEASFSAAN
                     EAIQIHGAYGFSDEYDVERYLRNSKGAMIYEGSSEVQTLIQAGYALGERADKPLRCEL
                     PAYDESHWQG"
     gene            complement(11326..11448)
                     /locus_tag="VFQ23_07135"
     CDS             complement(11326..11448)
                     /locus_tag="VFQ23_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906396.1"
                     /translation="MFVVAVVVCLLLFVALAGADLLVSNINSDELSNMGVEKKS"
     gene            11727..11810
                     /locus_tag="VFQ23_07140"
     tRNA            11727..11810
                     /locus_tag="VFQ23_07140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11761..11763,aa:Leu,seq:cag)
     gene            12049..14568
                     /gene="gyrA"
                     /locus_tag="VFQ23_07145"
     CDS             12049..14568
                     /gene="gyrA"
                     /locus_tag="VFQ23_07145"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET9906397.1"
                     /translation="MAEENNQAGNIQQVDIDSQMRDAYLDYAMSVIVARALPDARDGL
                     KPVHRRILFAMQELGIRSNSSYKKSARIVGEVLGKFHPHGDSAVYETMARMAQDFSMR
                     YMLVDGQGNFGSIDGDAPAAMRYTEARLHRLAEELLADIDKDTVDFGPNFDDSLEEPL
                     VLPARVPNMLLNGTSGIAVGMATNIPPHNLGELTDALIFLIDNYDRMDDIPVEDLMKF
                     VQGPDFPTGGIIVGREGIESMYGTGRGRIVVRGMAHIEEGKGGRHEIIITEIPFQVNK
                     TTLIERIAELVREDKIDQISDLRDESDQRGMSIVIELKRGAQPKKVLNQLYKFTSLQT
                     TFGAQILALVDGEPRTLPLKRSLQIFIEHRQTVIVRRTKFELAKARARQHILDGYLIA
                     LNNLDAVIKTIRESEDADIAKANLVSRFKLSEIQAQAILDMQLRRLAALERWKIEEEH
                     KQVREHIEFLEDLLASPKKILALIQTDLRDLAEKYGDDRRTRIAAEATESLSEADLVQ
                     DEAVLISLTERGYVKRVASSAFRSQSRGGRGVMGHTTKDEDEVVALIPARSLDTMLFF
                     SDKGKVYSEKVYQIPDADRTAKGIPLVNVLALDANERVTAAIAVSDFSAHGYCMLATA
                     RGKVKRVVMEEFAAVRPSGLIAVTLEDGDYLGWARVTSGNDEVIFVTENGQALRFNES
                     KVRSMGRQAAGVQAIALKNGDAVTSMDVVEKDGSLLVVTSNGYGKQTPLNAYPAKGRA
                     TGGVSTIDQKALPEIGKIVSARVVQNADDLTIITANGVAIRIKNKDVKQAGRATKGVH
                     LIKPQPGDSVASVARIAAEDLKRAGASLNGEEKEEPQPQLI"
     gene            complement(14626..14862)
                     /locus_tag="VFQ23_07150"
     CDS             complement(14626..14862)
                     /locus_tag="VFQ23_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906398.1"
                     /translation="MFDNLRDSAESSFYEEEPNDLYNEPTPKSKSAAPRRRSNGKLLG
                     MNAQQRFMISLMLLFTVCLVGTLAMLVLGKMSLF"
     gene            complement(14885..15664)
                     /locus_tag="VFQ23_07155"
     CDS             complement(14885..15664)
                     /locus_tag="VFQ23_07155"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET9906399.1"
                     /translation="MNSKEIHKTLSKLPLGDIKYFDVVGSTNDEALAWAANDAKDLSV
                     VIADEQTAGRGRLDRKWFTPPGTAFAFSVILRPTAQEKSHLSRIVGLAALAVANVLRE
                     HQLAPQIKWPNDVLLLERKVAGILVESVWSGEDVDCLVIGIGVNVLKGSVPPAHILNF
                     PATSLEDELGYSPDRLKLLSEILSAFINLRPFLNTEEFLNRWAESLAFIGEQVQVDLG
                     DEPSVFGQVLGLEQDGSLRLLDSQNKGVAVRFGDVRLRPLT"
     gene            complement(15709..16767)
                     /locus_tag="VFQ23_07160"
     CDS             complement(15709..16767)
                     /locus_tag="VFQ23_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9906400.1"
                     /translation="MPKLVSFFNSNRFNIISWFVTAILVVALISGTLIWRKSRAAPSA
                     LAPQPTASSEENQPEVEMPALGGPETFASIEREIQIKTNSPADKPRYEPVEYRVVRGD
                     SVFAISESYKLTPETILWANYDVLQDDPHSLQPGQVLTIPPTDGIYYQWKENDTLETV
                     SAEFAADVDNILNYPGNDIDLTNPKVDSGSWVMIPGGQREFVKWLVPTIASGASGTSS
                     TNQSACPGGAVGGGGFVWPASSHSLSGNDYWSGHLGIDIAAGAGEPVFAADSGVVTMA
                     QGGYNYGYGNVIQIDHGNGYSTVYAHLTAYFVSVCGSVSAGQQIGTAGNTGNSQGAHL
                     HFEVRQNGGFINPWFVLP"
     gene            complement(16789..18066)
                     /gene="rho"
                     /locus_tag="VFQ23_07165"
     CDS             complement(16789..18066)
                     /gene="rho"
                     /locus_tag="VFQ23_07165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET9906401.1"
                     /translation="MKILELENEPLSKLRGMAKGLNIPNSNRLKKETLAMMIREAEAQ
                     KEGIELRGGILEIMSEGIGFLRATNYRISDQDVYVSQAQLRRHDLRAGDLVIGQVRPP
                     RESERHFGLLKVESINGLDPEEASRRPVFETLTPIFPDKRFDLETDHDTLAPRLINLV
                     APIGRGQRGLIVSPPKAGKTTILKQIANSVSTKYPDVHLIVALIGERPEEVTDMDRSV
                     DAEVVASTFDEPVTSHVRTAELALERAKRLVEIGRHVVILMDSITRLARAYNLVVNPS
                     GRTLSGGMDPSALYPPKRFFGAARNIEEGGSLTIIATCLVDTGSRLDDVVFEEFKGTG
                     NMELHLSRKLQERRIFPAVDIERSSTRREDLLLGPDLLQRVWLMRRMYIQMISPQPAG
                     AGMDQSVATEAIVTRLDRARNNQEFLENLGRDS"
BASE COUNT         4471 a         4771 c         4516 g         4469 t
ORIGIN      
        1 gcgactgacc tctggtgatt gggacgatac cactccgtca cccagcccgg atggcaaaaa
       61 gatcgctttc acgtccaaca gaaatggatt ctgggatata tatctgctgg atttatcaac
      121 aggggacgtc acacaactga ctaatactcc tgagtatgag ggcgcgccta cctggtcacc
      181 cgatagctcg ttcctcgcgt ttgaagccta tcttgatgaa aatcttgaga tcctcgttgg
      241 acctgcagag aatccactgg acggtgcagt gcgattgacc accgcgccga catccgatca
      301 ttcaccttcg tgggctccgg gcggtcgcca aattgcgttc atctcaaatg gcgaggtgat
      361 tcttgcagat ctggatagaa ccgatagtgg tcgctttcaa aacctcagca acaccaccaa
      421 tgcttctgaa tcgcaccctg tctggtcgcc ggatggtcaa cggctcgcct gggcctcgtc
      481 ctcacaaaat atcgggcgca gcggcattta tatatgggat agagggcgaa atctgcctgc
      541 cacatggatc ggcgaagggg attggcccgc ctggaacaca gaggggagtc agatcatctc
      601 tacccttgcc gcgccaaata gcacctatct caccatttat tcgaccgatg gcaaactact
      661 acagccgctc attccatttc cgacaacatt gcgcgggctg atgtgggcaa atctagttat
      721 gaccgaatcg ctgccaggaa gatttcaaca ggcggcggaa ttcacgccag ctccgctttg
      781 ggcggcaaat ggtgaaccgg tttcagaagg tgcttctggc cgatggtcgc tcgtaaattt
      841 gcagggtgtg caggcgcctt acccgcaaat gcatgacctt gctgatgaag cctttgatgc
      901 cttacgtcaa cgtgtccaac aggaagtagg ctgggacgcg ttggccagct tggaaaatgc
      961 cttcgttccg attacaaccg tgcttgaccc tggttttcaa gaagactggt tatttaccgg
     1021 ccgcgccttt gcaatcaatt ctcttatgac gaacgccggc tggatggccg ctgtgcgcga
     1081 ggatttcggt tcacagacct attggcggtt atatctcagg gcgcagttgc aggatggttc
     1141 attgggagaa ccattgcgtg acgcgccatg ggatctaagt gctcgctata atcttgaccc
     1201 gaaggtctac gaacagggcg gaacatatag cgaagtacct gcgggatatt gggtggatgt
     1261 cacggcgctt gcatctcaat atgggtggga acgcgtgccg gctttgccaa actggcgaac
     1321 gttttatcga ggcgcgcgat tcacggaatt cacactgacc ggtggcttgg attggtattc
     1381 agccatgctt gaactatatc cgcctgatgt gctgctcaca ccgacgcgtg ttttaccccc
     1441 cacaactact cccacccgta cgcctctgcc aacatggacg ccccttccaa cgcgcacgcc
     1501 acgtccaacg tggacacctt caccgacgcg ttctccaacc gtaacagtca caccctcagg
     1561 gacaccgcct ccatctccca cgcctccgac ggtaattcca tgaagaagat gattattcgc
     1621 gctgagcgga aggacgatag tcctgtagac gaagcggttg tttcatataa tgcgcttcgt
     1681 cttcgctctg ctccgctcag cgcgatggta ttactttttt ttatcattat cctcactgcc
     1741 tgtactccgg cgacttcgac tgaaactgaa acaggatcaa ccccaacggc tgtgatcgcg
     1801 ggggaagtcg cgactcagcc tccggtccaa atagacgtgc agccgaagcc aaccatcgcg
     1861 ccctttcgat tctttcttcc cacgccaggc gcagagcccg tattaggatg gcgtcctccg
     1921 ctttacccca tcccctgggc tgtttcggca tatgaccatt tttactttgc gcgaccgatc
     1981 gcggcagata acgtcaactg gcccctggct gaatatcgtt atggcggtgt gttcttcgcg
     2041 cccaacatcg tgcataccgg cgtggatatc gacgcagccg aaggtactcc cattctggct
     2101 gctggcgctg gcaccgtcgt atccgcagac tgggggttat tcagtgaagt gcctggcaat
     2161 atctcagatc cttacggtca ggccgtagtc ttgaaacatg actttggtta tcaagatcga
     2221 tctctcttca cagtctatgc acacatgagc aagattattg cagtggttgg ccagcatgtg
     2281 gagaccggtg acgtgctcgg cctggtaggc gataccggtg ttacaactgg accgcatttg
     2341 cactttgaag tgcgcgtggg tggcaatact ttttacaaca cagccaaccc ggaattatgg
     2401 atggctccac cccaaggctg gggcgtctta gttggaagaa tgacagatga agacggcgag
     2461 ttgttgaatc tctatccggt cgaggtacgg cccatgcctt ctgaggtgcc gctgcgcaga
     2521 gcgttgacgt acgcgcaggg tgccgcgaac tctgatgcgt tttatcagga aaacctcgtg
     2581 ctgagtgatt tacctgccgg cttgtataag atcgcctttg aatataagga caaacagttg
     2641 caattctggg cggatattta tcctggacaa atcacttatt tcaccttcac agatgaagat
     2701 ggatttcaag tcgtcccacc acccatgcct acgttggatt ttctacccgg tacagcgacg
     2761 gttgtgcctt agagcatcaa tagcccttcg ttagcatgct atactattca gatccgtaga
     2821 gtataactcc cttgacgtcc agaacatctg tgctatactt cttgcctaca cacatttgac
     2881 ctttacattt gtatttggag aataacctat gtcccctcga agaaaatccg ctgctattga
     2941 agagcaagca cccagtcgca atagtaatgt agacggcgca aaacaggctg tcctagacaa
     3001 agctgttggt gatatcctca aacgttacgg tgatggctcc attatgcgat tgggcgaagc
     3061 ccatggcatg gtgaccgaag ccttccccac cggttcgttg tcgctggata tcgctttagg
     3121 cgtcggtggc gtcccgcgcg ggcgtatcac tgaaatctat ggacctgagt cgtctggtaa
     3181 gacgaccctc tgccagcaca ttgtggcaga ggcgcaaaag atgggtggcg ttgccgcgtt
     3241 catcgacatg gaacacgcgc ttgatccggt ctatgctgca cgtgtcggcg tggatatcga
     3301 caatctgctc gtctcccagc cggataccgg ggaacaggcg cttgagatca ccgaaaccct
     3361 cgttcgctcc ggtggcgtgg acgtgatcgt catcgactcg gtcgctgcgc tggtgccgcg
     3421 tgctgaaatc gaaggcgata tgggtgatcc ttccatgggt gtgcaggcac gtttgatgtc
     3481 tcaggcgctt cgaaaacttt ccggggcgat taatcagaca aaaacagcag tcatttttac
     3541 aaaccaactg cgccaaaaga tcggcgtgat gttcggtaac cccgaaacaa ccaccggcgg
     3601 tcaggcactc aagttctacg cctcgattcg tctcgacgtg cgccgcattc aggcgatcaa
     3661 agtgggcgat gaagtcatcg gcaaccgcac ccgcgtgaaa gtggtcaaga acaaggttgc
     3721 acctcccttc cgtacagcag agttcgacat catgttcaat gagggtatct ccaaatcggg
     3781 cgatgtgctg gacctcgcca cgaaattcga agttgttcaa aagcgcggcg ccttcttctc
     3841 ctatggcgac attcgcattg gtcagggtcg cgaaaacgca aaggattact tgcgtcagaa
     3901 tttcgatctg atgaacgaga tcgatacggt catccgccag aaagctctta gcggcgaaat
     3961 agcccttccg ctggatatgg ctgttgaagc aaacggcggg gcagccgaag aagaagaata
     4021 acaaagcaca gaagggcgac aaaacagtcg cccttttttt atttactcac tgcaccagaa
     4081 acattcggac tgtgaattca agctctatat gagctaaaaa tcaaagcctt ttataaaacc
     4141 agcggtaatg atatgcttcg gagtatgtca agccgatatc aagcacttca acatagggtt
     4201 gggctaacgg gcattctact gactgccctt ttcacctcgg cctgttcccc agctactacc
     4261 ccaactccat tccggccacc cactgcccag gctccactga ttgaaccaac attcattatc
     4321 aatcctacac agcccattgt tgttgtgcaa tcttcacccc tgccaacaat tatccccact
     4381 acacgacctg aagattgttc cagcaatcta acatttgtca gcgacctgac cattcccgat
     4441 aatacatcca tcacatacgg ctcaacgata gacaagcagt ggatcgtcga aaacagtggg
     4501 tcttgtcact ggggacctaa ttaccgtttg agacaaatcg ggggagctac gttgggtgca
     4561 cctgaagaag ttgcgttata cccagctcgt gcaggcaccc aggctacgat ccaaatctta
     4621 ttcactgcac ctttcacaga tggtgaatat gaaagtgcct ggcaggctgt tgacccgcag
     4681 ggtattgcat ttggcgatcc gatttttgtt cgaatcgtgg ctgtagcccc ataaggagtt
     4741 tatatggcga ctcaagctga ttttccggtt gtattcgaga aactcaaatc catccttaaa
     4801 ccgtacgcag aaacgcttac tgtaaaaacg gactcaatgg atacctatta cctggacggt
     4861 ccttataccg aaaaatggaa gaaggaactc ttctttggat ccgcacaaat caaaaagaat
     4921 tatgtgtcct tctatctcat gccggtatat atgtaccccg acctattgaa aggtatcagc
     4981 cctgacctta aaaaacatat gcaaggaaaa tcctgtttca atttcaaaaa ggttgagcct
     5041 gaattgctcc aggaacttac agagcttacc gagaaggggt atgcgcgatt taaacaggaa
     5101 gaactcttaa aataagcgga aggattcatg aagttcaaac ttgagatccc tataggcaag
     5161 cctcgtgatg aagtgtggaa agcctttgac aatatagaga atatgaagaa gtggcagcct
     5221 tcgcttatct cctttgaacc catcagcgga acacctggcc agccgggagc ggtctcaaaa
     5281 ctgacatatg aggagaacgg tcgtgaattc tctttgatcg aaaagatcac ccttcgtgat
     5341 gagccgcatc gcttcgacgg cgtttacgaa aacaactttg ccaataacat catcagaaat
     5401 cggttcattg aacatggcac agatcaaacg ttatgggtca cggagacaga atataagttc
     5461 aaaacgctgc tgatgaaaat cgccggacca ctgttgaaaa agaatttcgt ggcgcgtaca
     5521 aaaagagata tggagcgctt caaagacgtg gcagaaggaa aacccgacca agaaatggaa
     5581 aatatcgaaa aagaaccatg aaattgctca aaagaatcct gataggtatc ttgatcgctg
     5641 tcgtagcatt ggtcctactc ctcttcggtt ccattgcaat tgatgctgta gccagccgtg
     5701 gtcgtattga ccaggtggcg aatacagtca tcccaggctt gaacggtgga cccgatgtgc
     5761 gcgcctatgt agcacggccg caaggcgagg gacctttccc tgtagtgatc atgatccacg
     5821 aattcatggg gttgaatgat tcgatcaccg gcagggcgga tctgcttgcc gagtctggct
     5881 atcttgtgat tgcgccagac acgtttcgcg gctcgacgac ttcctggcta ccccgcgcaa
     5941 tctacctggt aattacgacg aaggctgaag ctgttaacgc cgacctggac tcagtctatg
     6001 catggctgga aacccaggag gatgtgtcca caaatcgaat cggcattgca ggtttttgct
     6061 atggcggacg tacatcactg gcatacagcc tacacaatcc aaaacttgct gcgaccgtga
     6121 ttttctatgg cgctcctgaa acagaagtgg aagtgttgaa gacgctgcca ggtccggttt
     6181 tggggatttt cggaggtgcc gacacttcaa tccccgtgga aactgttaat gccttcgaaa
     6241 gcggacttga acaggccggc ataccacatc aaatcacgat ctatgagggt caaccgcacg
     6301 cttttgtaag cgataaagag aagatcgaag ctggaggagc gcccgctgag gcatggaatg
     6361 aaatgcttga gttcctggaa gaaaacctca agaacaaagc cagtcaaaac atggatagcg
     6421 cgcctcttgt ctacaaagca ccgtttacct ggagatatta tcttgcgctg gcatttgaac
     6481 atgcgtttgg agaggcaagt catgttcatt gaccccgtcg tccccttatc actctagaat
     6541 ttaagccacg acatcctcaa tcacatttga ttgaggatgt cgtttatttt attgatttcc
     6601 ttatctactt acccagcttt ttcttaaacg cctctgtaag agcagggaga atttggaata
     6661 gatctcctac cactccataa cgcgcgactt taaatattgg ggcgtcagga tccttattga
     6721 tggcaactac catcttcgca gagcgaatcc ctgccaggtg ttgaattgcc ccggagatgc
     6781 ctgatgcgat atataaatcg ggcgcgacaa ctttacccgt ctgccctacc tgatttgaat
     6841 aagggatata gccagcatcc acagcagcgc gtgacgcacc taccgccccg cctaaaacgg
     6901 atgcaagatc accaacgagt ttgaatccct gttgcgcgcg ccagatttct gcctgcttat
     6961 catcgagccc ggcaggcggc tgaagcgatg gattgttcga tacgccacgt ccaccagaga
     7021 cgatcactcc ggcatcattc aggctgactc ccacttccgc cacgctgtat ccttcgacag
     7081 ttgagagtcc gtcagtcttt gcgtccacct ttgtaaccgt gcctgtcttg ccttcttgtt
     7141 gttcaggttt ctggaaggca cgtccgcgta atgtgatgaa ttgaggctgg ccagtagact
     7201 ttaccttctc aaacaacttt gcctcataga ttgggcgagt cacgacaatg ctgtcgccat
     7261 ttacgtccac ggcggttgca tcggtgatga cattggtgtt taaatccaag gcggacatcg
     7321 ccgccagttc acgggtacga gtcgtcgtcg gaaacaggat caggtccggg ctttgcgctg
     7381 aaacgagcgc agaaagcgtt gcggcatagg tctccgcacg ataatccaga agcgaggggt
     7441 cgtccgcgag cagaatttca tctgcgccat attcgaaagc ggactgcggc acgttatcca
     7501 cacccgaacc aaagatcagg gctgttgccg cgcccaggct cttcgcaagc ccaagcgctt
     7561 cccacgaacc aggctgtacc tcgcctttaa agtgatcgat gtaaacgaat gtcttcatag
     7621 cactttctcc gcgaggatct tgtcagcgag tttatcggca atctcggccg ggctttcacc
     7681 ggtgatgatt tcaacacttg tatcgcgcgc cggcgggttc atcacctcaa tgcgtgatac
     7741 gactgattcg ggtgcgctca ctccaagatc attcaacgac cagaccggga tttctgcttt
     7801 cgaggctttg cgaatgccca taaaggatgg atatcgcggc tcgccaatac tttggacaac
     7861 gctaattacg accggggctt tcgctttgac gatctgcctg ccttcctcga tgactcgttc
     7921 cacagtcaca gatccatcct ccgctttgat ctgcccggct aacccaagca tgggccaccc
     7981 catgacgcgc gctgtttgtg caggcgtgat acccgagccg ctatcgagcg tttgtcgccc
     8041 gaatacgacc atatctacgc tgccgatttt ttgaatcgca gcggcaatga tgcgcgctgc
     8101 cccaaccgta tccaggttgt tcagcgcggg gtctgagacg agaatcgcgt catctgcgcc
     8161 catagccagc gcgtgcttga gcgcttcctt cgcggattca ggcccgatgc acagcgcagt
     8221 gactgtgccg ctcgtggctt ccttctgctg cagcgcgcct tctaccgcgt actcgtcaaa
     8281 tgggttaatg accagtggcg catctcccca cgatacttgc ccaccctcgg ctcttacctt
     8341 cgcctctgag tcgggcactt gtttgataca cgcaactatt ttcaagagca actccttagg
     8401 atattagatt ttagcaagca gattcgcaaa actcccactg tttagaagtg gagtcatatt
     8461 ggtatttatg attccataat gttcggaatg atagtgtata tctatcgcca gttccatagt
     8521 actgatactg ataacgccag ttagcgtagg gaactttttt tagatttcca cttgaaacaa
     8581 gatatatgag attccaagga tactggttat atttatcttt atatgattcc aatgccacta
     8641 ttatttctct tgaattctga gttcctgtac gatatgttgg agcatctaga ctgtaaaacc
     8701 aaataaagga tgctgcacaa aggaaaatac atattattga aaaaacacga aaggctattg
     8761 tgaaagaact tttcattgcg acacgacaaa cgttatcacg ataaaccatg cgcagctgtc
     8821 attgcgagga cgcttcaagg ctcttcccca taaggcaatg tccaacacta tttgcggctc
     8881 catattgtga aggggattgc ttcgccctca cgggctcgca ataacgaaga attcttttac
     8941 tgaagactcg tcttgctgac gattttgcct gtgcctagct ctaactcgca ggcgtggctg
     9001 ccgattgtat atgtcgatat tgtcgcgcca tcttcgttga gcctgactcg tgaatataac
     9061 gtgttcgggt caggcttttc tgccttccaa atcactttgc cactgacaga aacacaatat
     9121 aagtttgatt cgcttcttgg gaatccgttt gagtcttcca atacgaccca tccatgcaat
     9181 gtttgaatcg ttttatccac ttttgtgtgt tgaggtttga tatccatctt ttgcttatta
     9241 agaagcctcc tcggcctgac gaacaatttc ctcgagctgt tcaagatgat ccgaagcatg
     9301 ccttagccct gaagcgaaac gggaaacagc agtatgtggc accgcacctt cagcaggaga
     9361 ataggtattt tccatataag gttcatcggg ccacatttcc aaacttgcga ggcacatgcg
     9421 acgcgattct tccagacgag cacggcaata ttcaatcgaa gtcgcctcac gccacgggac
     9481 ctcagagcgt gagcgtccgt gaaaggctac gcctcgagcc aattcagaag caatcgcagc
     9541 ggattcttcc atcgaagcag tcagatgaac aatgacatgg cccagagtcc atgaaatcgt
     9601 ctcctcacct tctacagcgt atggatccgc tgccttttcg tccaccggca cgaaggtaac
     9661 ctgagcatcg gtgcagtcgg cgatcaagcg cgagacttcg tcgtacatgt cgttcaattc
     9721 gcgacgcaag tcggctttgg tgaggcctat caccaaatct tgcagggtaa gtttcttttc
     9781 gcggaccggt tgaaagtcaa gcatagttta tccttgccag tgggattcgt cgtaggcggg
     9841 cagttcacag cgcagaggct tgtctgccct ctcacccaaa gcatagcctg cctgaatcag
     9901 agtctgaact tcagaactgc cttcgtaaat catcgcgcct tttgaattgc gcagataacg
     9961 ttccacatca tattcgtccg aaaatccgta tgcgccgtga atttgaatcg cttcgtttgc
    10021 cgcgctgaaa gaggcttcgg tcgcaaattt cttggcaaac gaggtctcac gcgtggggcg
    10081 ctttccttcg tttttgagcc agccgacctg taaataaagc aaacgcgcga tttcataatc
    10141 ctgcgacatc ttggcgattt tctcttgaat gagctggtgt tcagcaattg gcttaccaaa
    10201 cgtctttcgg ccctttgcgt aattcacgct tgcttccagc gcggctcgaa tgatgcctgt
    10261 cgctcctgat gccacagtgt aacgcccgtg gtcaaagcca gacatggtga tcttaaaccc
    10321 ctccccttct tcgccaatcc ggttttccac tggcactttc acatccgtga atgatatcca
    10381 acccgtggcg ccggcgcgca cacccagctt gccatgcagg tctcccgttt tgactccatc
    10441 actatgcaag tcaacgatga aggacgacaa accatcggat cctttccgag cggaagggtt
    10501 cgttttgacg gtaaccagcg cgaggtccgc tttggaggcg agcgagatcc acatcttttc
    10561 gccgttcagg atgtagtggt ccccatccct ttttgcggag gtggacatgg ccgccacatc
    10621 tgaccccatt cctggttccg taaatgcccc gcacccaatc tttttgccgc tcgccaacgg
    10681 aacaagaaat ttttgctttt gttcttctgt accccactga aagaggctca ttgaacacaa
    10741 accggtatgc accgacatcg ccacccgcaa tgaggtatcc atggcttcga gttcttcaca
    10801 cgccagcccc cacgagatgt aatccatgcc ctgcccgcca tagcggacag gaaaacaaat
    10861 accgagaata cccagttcgc ccattcgctt cagcgcaaaa ggaattggtt cctgtttgcg
    10921 atcgtattcc ttgatgaccg gtgcaatttc cttttgggcg aagtcccgca ccatcttctg
    10981 cgccatttga tgttcattgg agagagagaa gttcattgaa tcgtgattcc taagggaatt
    11041 tttgtttgat tatacccgtt gaagaccttt accatggtca gtaacccgct aagggagtgg
    11101 ataacttcgt gggcttagcc accgagatcg cagagacctg agagaatctt ctcaaagaac
    11161 tcagtgttct cccccggcac tgcccaccag ggcaagtgtg tggcgaatcc tactatagcc
    11221 catccattta gccctccacc cgccaatgac tgtcgtttta aataaaaaat gcccgacgcg
    11281 cttttgactt cgcatcgggc tagggattat gggtgtgact ttctatcatg atttcttctc
    11341 gacacccatg ttgctcagct catctgaatt gatattcgag acgagcaggt ccgcgccagc
    11401 gagggcaacg aacagaagca aacatacgac aacggcgaca acgaacatag ggtactcctt
    11461 tctttcactt gacatacaac ctattaaaca tctttgcatt caaaaagaca catcccctaa
    11521 cagtactgaa atgatttgat aatgttgcga cttttcaaga ttgcaaggac gaatcacgga
    11581 cgaattgcat tgaagttttg ttccacgatt taatacgttt ttggcaaggt gcagttgcta
    11641 cttaattaac gcaaatcttt tgaactttca ggcaatctgg ttgacaaaac ctatctttga
    11701 gataaaattc agtgcgcaat aaacaggccg aagtggcgga attggcagac gcgctacgtt
    11761 cagggcgtag tgagagcaat ctcatgaggg ttcaagtccc tccttcggca ctgaatcaga
    11821 ctcgctgaaa ggcgagtttt ttgttgcccc ttcccaacct ctccaaatcc cacaaaaggc
    11881 tcacgagtta gctcattttc agttgtcgga ggtgccggtc agggcggtgg gggtagtggt
    11941 ataattagat aaaattcagg tagttgcttc attagttaag acgcggagtc acagaaacta
    12001 ttccttcgtg tcttttttat gccaaaaccc cattttcaga gagaacagat ggctgaagag
    12061 aataatcaag ctggaaatat ccaacaagtg gacattgatt cccaaatgcg ggatgcctat
    12121 ctcgattacg ccatgagcgt gatcgtggcg cgtgctttgc ccgatgcgcg cgatgggctg
    12181 aaaccggtcc atcggcgcat cctgtttgct atgcaggaac tgggcattcg ctccaattca
    12241 tcgtataaga aatcggcccg tatcgtaggt gaagtactgg gcaaattcca tccgcacggt
    12301 gactcggcag tctatgaaac catggcgcgc atggcgcagg atttttccat gcgctacatg
    12361 ctggtggatg ggcagggaaa ctttggttcc attgatggcg acgccccagc cgcgatgcgt
    12421 tacaccgaag ctcggcttca ccgattggca gaagaattac tcgccgatat cgacaaggat
    12481 actgttgatt tcggtcctaa ttttgacgac tcgctcgaag agccattggt gttgccagcg
    12541 cgcgtaccaa atatgctgct caatggcaca tcggggatcg cagtgggtat ggcaaccaac
    12601 attccccctc ataacctcgg cgaattgacg gatgccctta tctttttgat cgataactac
    12661 gaccggatgg acgatatccc tgttgaagat ttgatgaaat ttgttcaagg acctgatttt
    12721 ccaacaggcg ggatcattgt tggccgcgaa ggaatcgagt ccatgtacgg gacgggccga
    12781 ggacgtatcg tcgttcgcgg tatggcacat atcgaggaag gtaaaggcgg ccggcacgag
    12841 atcatcatta cagagattcc ctttcaggtc aacaagacga ctctgattga acggatcgcc
    12901 gagctcgtcc gggaagacaa aatcgatcaa atttctgacc ttcgagacga atcagatcag
    12961 cgcggcatga gcattgtgat cgaactcaaa cgcggcgcgc agccaaagaa ggttctaaac
    13021 caactttata aatttacatc gttacaaacc acatttggag cccaaatact cgccctggta
    13081 gatggcgagc cgcgtacgtt accgctgaaa cgctccttac agatttttat cgaacaccgg
    13141 caaacggtca tcgtccgccg cacgaagttt gaactggcaa aagctcgggc tcgacagcat
    13201 atcctggatg gctatttgat cgcacttaac aatctcgatg ccgtgatcaa aacgattcgc
    13261 gagtctgagg atgccgatat tgccaaagca aatttggtgt cacgcttcaa attgagcgaa
    13321 attcaggcgc aggccatcct cgatatgcaa ttgcgcaggc tcgccgccct ggaacgctgg
    13381 aagatcgagg aagagcataa acaggtcagg gagcacatcg aatttctgga agacctgttg
    13441 gccagtccaa aaaagatcct tgcacttatc caaacggatt taagggacct ggctgaaaaa
    13501 tatggtgacg accgccgcac acgcattgct gcggaagcca ctgagtcgct cagcgaagcg
    13561 gaccttgtac aggatgaagc agttttgatt agtctgacgg aacgcgggta tgttaagcgc
    13621 gtggcgtcct ccgcatttcg ctcgcagagt cgcggcggac gcggagtcat gggtcacaca
    13681 acgaaagacg aagacgaagt tgtcgcgctc atcccggctc ggagcctgga tacaatgctc
    13741 ttcttctccg ataagggcaa ggtctacagt gagaaggtct atcaaattcc tgacgcagac
    13801 cgcaccgcca aaggcattcc gctggtcaat gtgctggcgc tggatgccaa cgaacgcgtg
    13861 acagccgcca ttgccgtgtc agatttttct gcccatggct actgtatgct ggcaaccgcg
    13921 cgagggaaag tcaaacgtgt ggtgatggag gagtttgcag ccgtgcgccc ctcaggtttg
    13981 atcgccgtta ctctggagga tggtgactat ctgggctggg cacgcgtgac aagtggaaat
    14041 gacgaggtca tcttcgtcac tgaaaacgga caggcgttgc gtttcaatga aagcaaggtc
    14101 cgttcgatgg gacgccaggc cgccggtgtt caggcaattg ccctcaagaa tggtgacgcg
    14161 gtcacgagca tggatgtagt ggagaaggat ggctcattgc ttgtcgtgac gtccaacggt
    14221 tatggcaaac agaccccgtt gaacgcctat cctgccaagg gacgggcgac cggcggagtc
    14281 tcaacgatcg accaaaaagc attaccagag atcggcaaga ttgtctcagc acgggtggtt
    14341 caaaatgcag acgatttgac catcatcact gctaacggcg ttgcgatccg gatcaagaat
    14401 aaagatgtta aacaagccgg gcgcgccacc aaaggcgtcc acctgatcaa gccgcagccg
    14461 ggggatagcg tcgcatcggt cgcgcgtatc gcggcagaag acttgaagcg agcaggagcc
    14521 tcgctgaacg gtgaagaaaa agaagaaccc cagcctcaac tgatataaaa agaaagccca
    14581 gcatttgctg ggctttcttt tcgttatttg aattaagttg tggggttaaa acagcgacat
    14641 ctttcccagg actaacattg ccaaagtccc aacaagacat accgtgaaca acaacattaa
    14701 cgaaatcatg aacctttgct gggcattcat ccccaggagc tttccattgc ttcgacggcg
    14761 cggcgcggca cttttgctct tgggtgtggg ctcattataa agatcgttcg gttcttcctc
    14821 gtagaaggat gattcagcgg agtcacgaag attgtcaaac atagttacct cggcgcagag
    14881 tatatcacgt cagtggtcgt agacgcacat ccccaaatcg tactgccaca cccttatttt
    14941 ggctatccag cagccttaaa ctgccatcct gttccagccc aagtacctga ccaaaaacag
    15001 acggttcatc tccgaggtcg acttgtacct gctcacctat gaacgccagc gactcagccc
    15061 accggttcag gaattcttcc gtattcaaaa atgggcgcag attaataaag gcagatagga
    15121 tttcggataa cagcttaagg cgatccggcg aataccctag ttcatcctcc aaagaggtcg
    15181 ccggaaaatt caatatatgc gccggtggaa ctgatccttt cagcacattt accccgattc
    15241 caatgacaag gcaatctaca tcttcgccag accaaactga ctctacgagg atcccagcga
    15301 ctttacgttc cagtaggagc acgtcattgg gccacttgat ttgaggggca agctgatgtt
    15361 ctcgtagaac gttggctaca gcgagggcag caagcccaac aatgcgtgat agatgcgatt
    15421 tttcttgcgc agttgggcga agaatcacag aaaaggcaaa agctgtgcct gggggtgtaa
    15481 accatttacg atccagccgt cctcgacctg cagtttgttc gtctgcaatc actaccgata
    15541 aatctttcgc gtcgtttgcc gcccatgcca gcgcttcatc atttgtggaa cctacaacat
    15601 caaaatattt aatatcaccc agcggaagct ttgatagggt cttatggatt tctttcgagt
    15661 tcattaatag ccaaatccta aaggactcgg agtccttcag gatttatttt aaggtaatac
    15721 gaaccaggga ttgatgaagc ccccattttg acggacctca aaatgcaaat gtgcaccttg
    15781 agaattgccg gtatttccag cggtaccaat ctgctgacct gcactaacgc tgccgcagac
    15841 actcacaaag taggcggtta agtgcgcata caccgtggaa tagccatttc cgtgatcgat
    15901 ttgaattacg tttccatatc catagttgta tccaccctgt gccatagtga ccacaccact
    15961 atctgctgca aagacgggct cccctgcgcc cgcagcaata tcgattccga gatgccctga
    16021 ccagtaatca tttccggaaa gtgaatgcga actcgcaggc catacaaaac cgccgcctcc
    16081 caccgcccca ccggggcagg cgctctgatt ggttgaggac gttcccgatg cgccactggc
    16141 gatcgttggc accagccatt tcacaaactc gcgctgtcca ccagggatca tcacccagga
    16201 gcctgaatcc accttaggat tggtcaggtc aatatcattt ccggggtaat tgagaatatt
    16261 atctacatca gcggcgaatt ctgcggacac ggtttcgagc gtatcatttt ccttccactg
    16321 ataatagatg ccatccgttg gcggtatggt gagaacctgc cctggctgaa ggctgtgcgg
    16381 atcatcctgc aagacatcgt agttcgccca caaaatggtc tccggtgtaa gtttatacga
    16441 ctccgaaatg gcaaacacgg agtcgccgcg caccacacga tactctacag gttcatagcg
    16501 cggcttatca gccggtgagt tcgtttttat ctggatttcc ctctcaatgg aggcaaatgt
    16561 ttctggtcca cccagggctg gcatctcgac ttcaggttga ttctcttctg aagaagccgt
    16621 gggttgagga gccagggccg aaggagcggc cctggatttt ctccatatca acgtaccact
    16681 gatcaacgcc acaacaagaa ttgcggtgac aaaccaactg ataatattga atcgattgct
    16741 attaaagaat gagacaagtt tcggcattca acaaatcgtt taagacgctt atgaatctct
    16801 cccgagattt tccagaaact cctgattatt tcttgcccgg tcaagacgtg tgacaatggc
    16861 ttccgtagca acagactgat ccatccctgc gccagccggc tgtggagaga tcatttggat
    16921 gtacatgcgg cgcatcagcc acactctctg cagcaaatcg ggacccagca aaaggtcttc
    16981 gcgacgcgtg gacgagcgtt cgatgtccac cgcggggaag atgcgtcgtt cctgcaattt
    17041 gcgggaaaga tgcaactcca tgttaccggt gcctttgaac tcttcaaaga ccacatcatc
    17101 caggcgggag ccagtgtcta caagacaggt tgcaatgatt gtcagagagc caccttcttc
    17161 gatatttcgc gccgcaccga agaaacgctt gggtggataa agtgcagaag gatccatacc
    17221 accggacagt gttcgcccgg aaggatttac aaccaggtta taagcgcgcg ccaggcgtgt
    17281 gatggagtcc atcaagatta ccacgtggcg gccgatctcc accaatctct tagcacgttc
    17341 taatgccagt tcggcagtgc gcacatgcga ggtgaccggc tcatcgaacg tggatgcaac
    17401 tacttcggca tctacggaac ggtccatatc tgtcacttct tcgggacgtt caccgatcaa
    17461 tgccacgatc aaatgtacat ctggatattt ggttgaaacg gagtttgcaa tttgcttgag
    17521 gatggtggtt tttcccgcct ttggaggtga aacaataagt ccgcgctgac ctcgtccgat
    17581 cggtgctacc aggttgatta accgtggagc aagcgtgtcg tgatcggttt cgaggtcaaa
    17641 tcgcttatcc ggaaagatcg gggtaagtgt ctcaaagacc gggcggcggg aggcttcttc
    17701 cgggtccaga ccgttgatcg attcgacttt cagcaacccg aaatgtcttt cgctctcacg
    17761 gggcgggcgt acctgcccaa tgaccaggtc tccggctctc aggtcatgac gacgaagctg
    17821 cgcctgggag acgtatacat cctgatcact gatgcggtag tttgtggcac gcaaaaatcc
    17881 tatgccttcg ctcataattt caaggatgcc accgcgcagt tcgattcctt ctttctgcgc
    17941 ttccgcttcg cggatcatca tggcaagggt ctctttcttc aggcggttgg aattgggaat
    18001 gtttaatccc tttgccatgc cgcgcaactt ggaaagaggt tcgttctcaa gttctagtat
    18061 tttcatgtct gtttctcgtt gggtggggtg ttggaaagtt caatggacca gcattttcca
    18121 ggctggtaat gtcccttagg ttgtggctga atgtggattt tcggtcaaat tctgtttaca
    18181 cgtaacccgt gcaggttagc tcgccataca tctggattac gcactca
//
LOCUS       DASTJP010000237         4861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_5251,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000237 DASTJP010000000
VERSION     DASTJP010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5251"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /gene="npdG"
                     /locus_tag="VFQ23_07170"
     CDS             complement(<1..525)
                     /gene="npdG"
                     /locus_tag="VFQ23_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET9906402.1"
                     /translation="MTSESLLLTIAILGGTGREGKGLAYRWAKAGYQVLIGSRSSEKA
                     VTTASEIMELLEGSSSLVGTSNHEAAKLSDIVVLSVPYAAHRETLENVKDVLKGKILI
                     DVTAPLGSPKNKVQMPPAGSAAQEAKEIVGEDVEVVAAFQTIPYEHLLKDEEINCDVL
                     VTGTSKEARSEVLKL"
     gene            complement(535..1878)
                     /gene="ssnA"
                     /locus_tag="VFQ23_07175"
     CDS             complement(535..1878)
                     /gene="ssnA"
                     /locus_tag="VFQ23_07175"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HET9906403.1"
                     /translation="MLITNATVITWESENRVLEDHAILIENDRIREIGRTTELRARYP
                     QIQVMDARGQYVMPGSICAHTHFYGAYARGMAIPGTAPRDFPEILNRLWWPLDRSLDQ
                     ESIQYSVLPCLVDAIKHGTTTLIDHHASPNAIDGSLDIIAEAVEQAGLRAVLCYEVTD
                     RDGESKMKAGINENVRFIKKIKSPLLAGTFGLHASLTLSESSLELCRQNLPDEVGFHV
                     HAAEHEADEYDSLNKSGLRVVDRLHKHGILGPKTITAHGVHFDAREMEILAETGTWLS
                     HQPRSNMNNGVGVAQIESMMRAGIKVCLGNDGFSNAMWEEWKAAYLLHTVHHRDPRRM
                     GGYDVTQMAIYNNAALANVFFPDATIGQIVPGAYADLIFVDYQPNTPLTAGNLPWHII
                     FGFQQSTVTTTIVAGNVLMKDRELLTLDEKEISAKAREIAPKVWDRYQSEVAKTL"
     gene            complement(1880..4429)
                     /locus_tag="VFQ23_07180"
     CDS             complement(1880..4429)
                     /locus_tag="VFQ23_07180"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9906404.1"
                     /translation="MSFLDPISFVIGFAVGILFTWLVNRARPLLQQIREGWKQQREVA
                     QTRRTSGLEDNHRRLTLRRAQGMHLAASLFSLNEILQEPRLIAPPASVEPGVIGIPED
                     IISQTLPYMPAWPELAAIYQAPTLDMTEAIAGGANIVIVGAAGTGKTVALAHLASMAA
                     NLQVRLEPDKEAEAIPYLYHVADLHLPFDSSKDPINIVIDAASERAPVFDLRKLPAFV
                     QSSFKNGQALLLIDGFDELDPQGQTNVVEWFKALLQAYPKVRIVTTGCTHQLNGLIGI
                     GFNPLALMVWASDRTSRFIQAWGELWSRTVALDSFTQNGPEQVDPMLINTWLTTDHNG
                     LTPFELTLKVWGAYAGDSLGPRVMDAIATHVRRVAPAGTPVAALELLAMQVVLTAQPI
                     FDPRTAQRWVKQYDIVEDQPTNSSQSLTDTADGKSIALTESQKIRIKKSKAGEAVPNQ
                     GLLQKMVESGLLLAHSNNKLRFMHPVLNGYLAGQAIGDNDADTTLLAQPEWDGKTVSL
                     RYLAARGDASAVADIMLKESDLPLHHQVFAVARWLRDAPKEAAWRAKVFSSLLTILQA
                     EGQPIGLRGQAMAAFVMSGDPGAATLFRQLLSSRSFELIPLAALGSGAMRDVKAIEAL
                     EEVMQAPSGAIRRATCLALVAIGTEKSLEGVARALLQGDEELRRAAAEALANDPAEGH
                     PMLKEGATLADIMLRRAVVHGLSRIDRPWAVETLQKMQVQDDQWAVRNLANQYLEQMG
                     KIDARVPRQLKTPSEAPWLIEFAGKQGVGIPRGGPATDVLLNAFKSGETEQRLAALPY
                     LKRVANEGIIGALYNAMYGEDAEVREAAFYAIEEIGSNGNKLPHPNQFGLG"
     gene            complement(4436..>4861)
                     /locus_tag="VFQ23_07185"
     CDS             complement(4436..>4861)
                     /locus_tag="VFQ23_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET9906405.1"
                     /translation="LQKDVASLKRYLETLEERELEAMLVVETSENESKAAKANLDHVQ
                     SNRSDQNRDLMQESELLHKNLERLNTERQAVIQNLAQQKMGVYDQLRQQRRGIAVTTI
                     SDAACAACGTTLTPSQQQNARSTSQLFHCPSCGRILYAN"
BASE COUNT         1115 a         1309 c         1188 g         1249 t
ORIGIN      
        1 cagttttagg acttcactgc gcgcctcctt gctcgtccct gtgactaaga catcgcaatt
       61 tatctcttca tctttcaaca aatgttcata aggaatcgtc tgaaaggccg caaccacttc
      121 cacgtcttcg cccacaatct cttttgcctc ttgcgctgcc gagccagccg gaggcatttg
      181 caccttgttt tttggtgatc caagcggggc agtgacatca atcagtattt ttcctttgag
      241 cacatctttt acattttcga gggtttcgcg atgtgcagca tatgggacac tcaacacaac
      301 aatgtcggaa agtttggctg cctcatggtt ggatgtacca accagggaag atgatccctc
      361 gagtaattcc atgatttcag atgcggttgt caccgctttc tctgagctgc gagagccaat
      421 taacacctgg tatccagctt ttgcccagcg ataggcaaga cctttccctt ctctgccagt
      481 tccgccaaga atggcaatcg ttaataataa tgattcactg gtcatcaaat tctcctataa
      541 agtttttgct acttcacttt gatatctatc ccaaaccttg ggagcgattt cgcgcgcttt
      601 cgctgaaatt tccttttcat ccaacgtcaa cagctctcga tccttcatca agacatttcc
      661 tgcgacgatg gttgtcgtga ccgtgctttg ctgaaaacca aaaataatat gccacggcag
      721 attcccggcg gtcaacggtg tgtttggttg gtagtcaaca aatatcagat ctgcgtacgc
      781 gcccgggacg atttgcccaa tcgttgcatc aggaaaaaaa acatttgcta acgcagcgtt
      841 gttgtagatc gccatctggg tcacatcata cccgcccatt cgacgcgggt cgcggtgatg
      901 gactgtatga agcaaatagg cggctttcca ctcttcccac atcgcattcg aaaacccatc
      961 gtttcccagg cagaccttga tgccagcacg catcatcgac tcgatttgtg ccacaccgac
     1021 accattgttc atatttgagc gtggttgatg cgaaagccat gttccggtct ctgcgaggat
     1081 ttccatctcg cgcgcatcga aatggacgcc gtgagccgtg atcgtctttg gtccgaggat
     1141 tccatgcttg tgcaaacggt ccaccacgcg taatcctgat ttattcaggc tgtcatattc
     1201 atccgcttca tgctcagcag cgtggacgtg gaagcctact tcgtcaggca ggttttggcg
     1261 gcacaactct aaagaggatt cagaaagcgt gaggctggca tgcaatccaa aggtcccggc
     1321 aaggagtggg ctctttattt tcttgataaa acggacgttt tcattgatcc ccgccttcat
     1381 ttttgactca ccatcgcggt cagtaacttc gtagcacaaa accgcgcgca acccagcctg
     1441 ttcaaccgcc tccgcaataa tatccagaga tccatcaatt gcattgggtg aggcgtgatg
     1501 gtcaatgagc gtcgtcgtgc catgcttgat ggcgtcaacc aggcacggaa gaactgaata
     1561 ctggatactt tcttgatcca gcgaccgatc caacggccac cagagcctgt ttagaatctc
     1621 aggaaaatcc ctgggcgccg tgccgggaat cgccatacct cgtgcatatg caccataaaa
     1681 atgtgtatgg gcgcatatgc tccctggcat tacatattgg ccgcgtgcat ccattacttg
     1741 aatttgagga taacgtgcgc gcagttcggt tgtcctgcca atttctcgaa tgcggtcgtt
     1801 ctcaatcagg attgcgtgat cctcgagaac gcggttttct gattcccagg tgatcactgt
     1861 tgcattggtg atgagcatat caacctaacc cgaactgatt ggggtgaggt aatttatttc
     1921 cgttggagcc aatttcttca atcgcataaa aggcggcttc tcgcacctct gcatcttcac
     1981 catacattgc attgtatagc gcgccgatga ttccctcatt tgccacacgc ttgagatagg
     2041 ggagcgcagc caggcgctgc tccgtctccc cggacttgaa tgcgttcaaa agcacatccg
     2101 tcgccggtcc gccgcgcgga atgcccacac cctgtttgcc tgcgaactcg atcagccagg
     2161 gagcttccga tggagtcttc agctggcgcg gtacgcgtgc atcaattttt cccatctgtt
     2221 cgagatattg attagccaga ttacgaactg cccattgatc atcttgtacc tgcatttttt
     2281 gcagggtttc gacggcccag gggcggtcga tccgtgacag tccatgaacg accgcgcggc
     2341 gcagcataat gtctgcgaga gtcgcgccct ctttgagcat gggatgccct tccgcaggat
     2401 cattcgccag cgcctctgct gcagcacgcc tgagttcctc atcgccttgt agcagggcgc
     2461 gtgcgacacc ttccaatgat ttttctgttc caatcgcaac cagcgctaga cacgtagcac
     2521 gccggatcgc gccggatggc gcctgcatca cttcttccaa agcttcgatt gccttaacat
     2581 cgcgcatcgc accgcttccc aaagcggcca atggaatcaa ttcgaaggac cttgaactga
     2641 gcaactgacg gaatagcgtc gcggcaccag gatcaccgct cataacaaaa gcagccatcg
     2701 cttgcccacg caagccaatc ggctgacctt ccgcctgcag gatagtcaat aaacttgaga
     2761 agactttcgc acgccatgct gcttcttttg gtgcatcgcg aagccagcga gcgactgcaa
     2821 agacctgatg atgaagtgga agatcagatt ccttcaacat aatatcggca acggctgaag
     2881 catcaccgcg ggcggcaaga tatcgcaatg agaccgtctt tccgtcccat tcgggctgtg
     2941 caaggagggt tgtatcggca tcattgtcgc caattgcctg ccctgccaga tatccattca
     3001 gcacaggatg cataaatcgc agtttgttat ttgaatgcgc cagcagcaat ccgctctcga
     3061 ccattttctg caagagtccc tggttaggaa ccgcctcacc agccttcgat ttcttgatgc
     3121 gtattttctg cgattcagtc agtgcgattg atttgccatc tgctgtgtcc gtgaggcttt
     3181 gtgaagaatt tgttggttga tcctcgacaa tgtcatactg cttgacccat cgttgggccg
     3241 tgcgcgggtc gaagatcggc tgggcagtca gaaccacctg cattgccaat aattccaaag
     3301 cagcgacagg cgtgcccgct ggcgctacgc ggcgaacatg agttgcgatg gcatccatga
     3361 cgcgcggccc tagactatca ccagcgtacg caccccacac tttaagagta agctcaaacg
     3421 gagtcagccc attatgatca gttgtcagcc aggtgttgat cagcatggga tctacttgtt
     3481 cgggtccgtt ttgtgtgaag gagtccaagg cgacggttcg tgaccatagt tcaccccacg
     3541 cctgaataaa acgtgaggtt cgatcagaag cccataccat aagagccagc ggattaaaac
     3601 cgatgccgat caatccattg agctgatgag tgcagcccgt tgtaacaatt cgaaccttgg
     3661 ggtatgcctg tagtaaagct ttgaaccatt caacgacatt ggtttgcccc tgcgggtcaa
     3721 gttcatcaaa cccatcgatc aataataagg cctgcccgtt cttaaaacta ctctggacaa
     3781 atgccggcag cttcctcaaa tcaaataccg gtgcgcgttc acttgctgca tcgatgacga
     3841 tattgattgg atccttggat gaatcgaaag gaaggtgcag gtctgctaca tgatacaggt
     3901 atgggattgc ctccgcttct ttatccggtt caagccgaac ctgcagattc gcggccatcg
     3961 acgccagatg cgcgagcgcc actgtttttc ctgtccctgc cgcgccgacg atgacaatat
     4021 ttgccccacc tgcaatggct tcggtcatgt ctaaggttgg agcctgataa attgcagcaa
     4081 gttcaggcca ggcaggcata taaggtaggg tttgcgaaat gatgtcttct ggtatgccaa
     4141 tcacgcccgg ctcgacactt gccggaggcg caatgagacg tggttcctgt aagatttcat
     4201 tcaatgaaaa taacgaggcc gccagatgca ttccctgtgc gcgccgtagc gttagacgac
     4261 gatggttgtc ttccagccca cttgtgcgac gagtttgtgc gacttcgcgc tgttgtttcc
     4321 agccctcgcg tatctgctgg agcaggggac gcgcgcggtt gaccagccat gtaaacagga
     4381 tgccgaccgc aaagcctata acaaatgaaa ttggatcaag aaaactcatc gaagcctagt
     4441 ttgcatacaa tattcgacca caggaagggc agtggaacaa ctggctcgtg gagcgcgcat
     4501 tttgctgttg tgaaggcgta agtgtcgtac cacatgcagc gcaagcggcg tcactgatgg
     4561 ttgtgacggc gatcccacgt cgttgttgac gaagctgatc atatacgccc attttctgtt
     4621 gcgcgagatt ctgaatcaca gcctgacgtt cggtattcaa ccgttcaaga ttcttatgaa
     4681 gaagttcgct ttcctgcatc agatcacgat tttgatcgct ccgattggat tgtacgtggt
     4741 cgagattcgc ttttgcagct ttagattcgt tttctgacgt ttcaaccacc agcatcgctt
     4801 ctaattcacg ttcttccagg gtttcaagat aacgtttcag agaagcgacg tctttttgta
     4861 a
//
LOCUS       DASTJP010000238         6951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_356605, whole genome shotgun sequence.
ACCESSION   DASTJP010000238 DASTJP010000000
VERSION     DASTJP010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356605"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="VFQ23_07190"
     CDS             <1..1565
                     /locus_tag="VFQ23_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906406.1"
                     /translation="PLMESTDNSRASAINILYSLGKSVLLHRASKMIQYGFLEVHDKE
                     NSLYVKVSDEAKDHFLDNIEFFQLEELENKLTDESVSFVNGWHLFDRVDYPNIEKALG
                     NFISRPLKDPFKDLKTEDVNKLMAPLIHPWDTGHGIMMGYGALPEIDDHFMAEAAELV
                     AEWRSEAGLHPDAKFDGISGADITSIITYITFLHLKHIRFSLVALETQPQISIPQSLT
                     IWEPLEKLEDSIVDFLEMDRTVAKKALHAITISPNEASYLHGVTKNFLPLLISLGNGL
                     VLRPVASLIKNPFASTIALQIWRNPKSINQLSLHREEWMRSEIYSIFQGTRYQRVHGN
                     VKLRSNNKIVTDIDAAIFDVVTGELALFQIKWQDYFTNDIRELRSKASNLTNELDEWA
                     EQVASWIQRHGHKELIKTLRLRLGESMVISSVYLFGVSKSYARMQGFGYTTKNQNLAI
                     ANLPQFHRIRYEVGPANQVFHNMFLAFRQEMTMSARLSKPLPVTITVSKKTVCFEDFW
                     NAYGDEEDEASE"
     gene            1840..3513
                     /gene="ettA"
                     /locus_tag="VFQ23_07195"
     CDS             1840..3513
                     /gene="ettA"
                     /locus_tag="VFQ23_07195"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET9906407.1"
                     /translation="MSNESLVIYSMNKVGRLVPISNKMILRDISLGFYYGAKIGVLGL
                     NGAGKSTLLRIIAGVDKDYIGEISQAKGYTIGLLEQEPKLDESKTVIEVVKEAVVPIV
                     EVLARYDEVNAKFAEPDADFDALVAEQAKLQEQLDKHDAWNLDSRLELAMDALRCPPS
                     DTPINICSGGEKRRVALTRLLLTEPDILLLDEPTNHLDAESVAWLEHHLRDYKGTVIA
                     VTHDRYFLDNVAGWILELDRGYGIPWKGNYSSWLEQKEERIRLEEKAESKRQKTLQRE
                     LEWIRMSPKARQSKGQARVSAYEKLLNQEAEARREELDIYIPPGPRLGDVVFEFDKVT
                     KSYGDRLILDGFSATVPPGAIVGVVGPNGAGKTTLLKMIIGQEKPDSGTVKIGETVKL
                     AYVDQSRTLDPEKTVYEEISQGADTLELGKRKINSRGYVSTFNFAGTDQQKKVGILSG
                     GERNRVHLAKTLTEGANVILLDEPTNDLDVNTLRALEAALEEFAGTALVVSHDRWFLD
                     RICTHLIVFEGDSQAKMYVGNWSDYETMMQEKFGKDLTPHRVKYRTMTR"
     gene            3552..3878
                     /locus_tag="VFQ23_07200"
     CDS             3552..3878
                     /locus_tag="VFQ23_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9906408.1"
                     /translation="MLELTTQNFQSEVLESDKPTLVYFYSPVGCAPCKLLSPVVEKLD
                     EEWNGAVKVGKLNIYPNMPITLKYTIMTAPVILLFKSGEPVLRLQGYHPHERLLAEMN
                     TYLTGI"
     gene            4001..4747
                     /locus_tag="VFQ23_07205"
     CDS             4001..4747
                     /locus_tag="VFQ23_07205"
                     /inference="COORDINATES: protein motif:HMM:NF036707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906409.1"
                     /translation="MPTLQPTSLNYPITESFIRFRDWKPLPPLSFNWQMKPVSSASKA
                     EIKVLPNNQFELTISHDIIKGVTPQMLHWWFCRIDDNMAYNGQSHHRYRVWHPLDHIY
                     YKDLSCAPDGTGSAGTRRHIVEAFGANPRYLINIIDRVAHLDDQGILLSTEQAGIALG
                     FSNLLMPLPLQLSTLEHQFFAVPNGTRYESRLLIGLDSTTGKWFNSIIRPLFIGYEMA
                     KAWLKHNIEEVGNFEFFLPELYEKQNKQDR"
     gene            4936..>6951
                     /locus_tag="VFQ23_07210"
     CDS             4936..>6951
                     /locus_tag="VFQ23_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9906410.1"
                     /translation="MAKAKPPSRKNKDMTGEKEHQHLIEAILDVEPGIVYIHDLQTNK
                     HVFINRDWLTNDTYKTAQNQADENLLPNLIHPEDLPRVTAHYDQLRTSSDEQGVHQME
                     YRVLQKNGEWRWVQSRDTIFSRNAQGEVTQILGILYDITESKQADEQMYFQAGLLAQV
                     NDAIIASDENSRITTWNSAAELMYGWKADEVIGQIGAEIVKTEFNGLNRADVLNILAT
                     EGKWRGEVTQARRDGSRFFVETASIVLRNMHGVITGYASVNRDITARRQAEEKLARQN
                     KRLTVLREIDAAILAADSIERIVDAALSHVRQLIECKRAAVILFDWEKQEAVFYNVQT
                     EGETANPKGTRIPLASSQWMIQIVSKNQPLVINELTMLPEPIPQVQQMIQAGFHSICI
                     LPLFVQNSLIGVFSMSSEMSGFFDSEKMDLGREVANQVSIAITQSRLVSELEEHLRER
                     EILIADLTAKNTELERFIYTVSHELKSPLVTINGYLGYLEQDAVIGNLERIREDTQRI
                     SSAVENMKELLNDLLDLSRIGRLMNPSQKIPFEEVVRDALEIAHGQLEAHSIAVHSQL
                     DLPIVYGDRLRLTEILQNLLDNAAKYMGEQPAPTIEIGQQEDAEGQFIFFVRDNGIGI
                     APEYHERIFGLFDKLDSRSEGTGVGLALVKRIIEFHGGRIWVESEGVP"
BASE COUNT         1974 a         1756 c         1618 g         1603 t
ORIGIN      
        1 ttccattgat ggagagcact gataacagca gggcttctgc cataaacatt ctttatagtc
       61 ttgggaaatc cgtgttgctt catcgggcgt caaaaatgat tcaatatgga ttcttggaag
      121 tgcatgacaa agaaaacagc ctttacgtta aggtatccga tgaggcaaag gatcatttcc
      181 ttgacaatat tgaattcttt caacttgaag aattggaaaa caaattgacc gatgagagtg
      241 ttagttttgt aaatggttgg catctctttg acagagttga ttatcctaat atagaaaaag
      301 cacttggaaa ctttatttca agaccactga aagatccatt taaagatcta aagaccgaag
      361 atgtcaacaa actaatggca cctttaatac acccctggga cactggacat ggaataatga
      421 tggggtatgg agcgcttcct gaaattgacg atcattttat ggcagaggcg gcagaattgg
      481 ttgctgagtg gagatctgag gcggggctac atcctgatgc aaagtttgat ggaataagtg
      541 gcgctgacat aaccagcata ataacctata taacattttt gcatctcaag cacataagat
      601 tctcgttggt agctcttgaa actcaacctc aaatatcaat acctcaaagc ctaacgatat
      661 gggaaccatt ggaaaaactt gaagattcaa tagttgattt tcttgaaatg gacagaacag
      721 tagccaaaaa ggcattgcat gccattacga tttcccccaa cgaagcctca tatttacatg
      781 gtgttacaaa aaatttcctt ccattattaa taagtttggg caatgggctt gtactaagac
      841 ctgtagcaag cctaatcaag aacccatttg catcaaccat agctttacag atatggagaa
      901 atccaaaatc cataaatcaa ttatccttac atcgtgaaga atggatgaga tccgaaatat
      961 attccatttt tcaagggaca cgttatcaac gggtacatgg taatgtaaaa ctgcgaagta
     1021 acaataaaat cgttaccgac atagacgcag cgatttttga tgtcgtaact ggggaactgg
     1081 cgctatttca aataaaatgg caggattact tcacaaacga tattcgtgag ttaagaagta
     1141 aagcgagcaa tctaacaaat gaactagatg aatgggctga gcaagtagca tcatggattc
     1201 aaagacatgg gcataaagaa ttaataaaga cgcttcgctt gagattaggc gaaagcatgg
     1261 tcatttcatc ggtgtactta tttggtgtat ctaaatcata tgctcgtatg caagggtttg
     1321 gatatactac taagaatcaa aatttggcga tagcaaacct acctcaattt cacagaattc
     1381 gttatgaagt tggtcctgct aatcaggtgt ttcataacat gtttcttgca tttaggcagg
     1441 aaatgacaat gtcagctcgt ctgtctaaac ccttaccagt aacaattaca gtttcgaaaa
     1501 aaaccgtttg ctttgaagat ttttggaatg catacggaga tgaagaagat gaagcatcag
     1561 agtaagtgta tcgcttacaa aattagatgt ttactttgca acttcaagtc cgcttcgagc
     1621 ggacttcgta aaaatagcac gggtgattca tccccgtgcg ggctaacaga ccaagacaat
     1681 tcctctcgaa ccagaacccg cctccagcta agaatgaagc gggtttttca ttcccaaaac
     1741 cattcatcat tcactattcc acattcttca ttcctccctc cactctctga tacaatactc
     1801 caccaaactc tagtcaccta ctctctaaca actaacctca tgtcaaacga atcactcgtc
     1861 atctactcca tgaacaaagt ggggcgcctc gtccccatca gcaacaaaat gatcctgcgc
     1921 gacatctcgc tcggcttcta ctacggcgcc aagatcggcg tactcgggct taacggcgcg
     1981 ggcaagtcga cgttactgcg catcatagcg ggcgtcgaca aagactacat cggcgaaatc
     2041 tcgcaagcca aaggctacac catcggtctg ctcgagcagg aacccaagct cgatgaaagc
     2101 aaaacagtca ttgaggtcgt gaaagaggcg gtcgtgccca tcgtcgaagt gctggcgcgc
     2161 tacgatgagg tcaatgccaa atttgccgaa cccgatgccg acttcgatgc cctcgtcgcc
     2221 gagcaagcca aactgcagga acaactcgac aagcacgacg cctggaatct cgacagccgc
     2281 ctcgaactcg ccatggacgc cctgcgctgt ccgccgtcgg atacgcccat caacatctgc
     2341 tcgggcggag aaaagcgccg cgtcgcgctc acccgactcc tgctcaccga acccgacatc
     2401 ctcctgctcg acgaacccac caaccacctc gacgccgagt ctgtggcgtg gcttgaacat
     2461 cacctgcgcg actacaaagg caccgtcatc gccgtcaccc acgaccgcta cttcctcgat
     2521 aacgtcgcag gctggatcct cgaactcgac cgcggctacg gcatcccgtg gaagggcaac
     2581 tactcctcct ggctcgaaca aaaagaggaa cgcatccgcc tcgaagagaa ggccgaatcc
     2641 aaacgccaga agaccctcca gcgcgagctc gaatggattc gcatgtcccc caaagcgcgt
     2701 caatccaagg gacaagcccg cgtcagcgcc tacgaaaaac tgttgaacca ggaagccgag
     2761 gcacgccgcg aagaactgga tatctacatc ccgccgggtc cgcgcctcgg tgacgtggtc
     2821 ttcgagtttg ataaggtgac caagtcgtat ggggatcggc tcattcttga cgggttttct
     2881 gctaccgttc ccccgggcgc tatcgtcgga gttgtgggtc cgaacggcgc aggcaagact
     2941 accctgctca agatgatcat cggacaggaa aaacccgaca gcggcactgt taagatcggc
     3001 gaaaccgtca agctggcgta tgtcgatcaa tcccgcaccc tcgaccccga gaagacagtc
     3061 tacgaagaaa tttcgcaagg cgccgataca ttggaacttg gtaaaagaaa aatcaattca
     3121 cgtggctatg tctccacgtt taacttcgcg ggcaccgatc agcaaaagaa agtcggcatc
     3181 ctctcaggcg gagaacgcaa ccgcgttcat ctagccaaga ccctcaccga gggtgccaac
     3241 gtcatcctgc tcgacgaacc caccaatgat ttggatgtaa acaccctgcg cgccctcgaa
     3301 gccgccctcg aagaatttgc aggcaccgcc ttagtcgtca gccacgaccg ctggttcctc
     3361 gaccgcatct gcacccatct catcgtcttc gaaggtgact cacaagccaa aatgtacgtt
     3421 ggtaattggt ctgattacga aacgatgatg caggaaaaat ttggcaaaga tttaactccg
     3481 catcgagtca aatatcgaac gatgacgcga taatgtctcg tagggacacg atatatcgtg
     3541 tccctactat catgctcgaa ctcaccaccc aaaacttcca gtcagaagtc ctcgagagcg
     3601 acaagccaac tctcgtttac ttctactcgc ctgttggttg cgcaccctgt aagctgcttt
     3661 cgcccgtggt ggaaaaactg gacgaagaat ggaatggcgc ggtcaaagta ggcaaactca
     3721 acatctatcc caacatgccg atcacactca aatacaccat catgactgcg cctgtcatct
     3781 tgctcttcaa atcaggagaa cctgtcctgc gcttgcaagg ctatcatccc catgagcggc
     3841 tcctcgctga aatgaacacc tatttgacag ggatttgagc ctgctgacag caacaggtcc
     3901 gattacgaga ccatgatgat ggggaaattc ggcaaagacc ttactccgca tcgggtgaaa
     3961 tctcgaacgt tgaaacgata atgtccctac aatattaacc atgcctacgc tacaacccac
     4021 ctccctcaac taccccatca ctgaatcctt catccgcttt cgcgattgga aaccccttcc
     4081 gcctttgtcc ttcaactggc agatgaaacc tgtctccagt gcctccaaag cggagatcaa
     4141 ggtcctgccc aataaccaat tcgagttgac catctcacac gacattatca aaggtgtcac
     4201 accacaaatg ttgcattggt ggttctgccg cattgatgac aacatggcat acaatggtca
     4261 gtctcatcac cgttatagag tctggcatcc ccttgaccat atctactaca aagacctttc
     4321 ctgcgcgccc gatggcacgg gcagcgctgg cacacgccgt cacatcgtgg aagcctttgg
     4381 cgcaaatccg cgttacctga tcaacatcat cgaccgcgtc gcgcatctcg acgatcaagg
     4441 tattctgctc tccaccgagc aggccgggat tgcgcttggg tttagcaacc tgctcatgcc
     4501 tttgccgctc caactctcca cgctggagca tcaattcttc gccgtaccga acggcacacg
     4561 ttacgagtcg cgtttattga tcgggttgga ctcgactacc gggaaatggt tcaattcgat
     4621 catccgccca ttgttcatcg gttacgaaat ggcgaaagcc tggctcaagc acaacatcga
     4681 agaagtgggg aattttgagt tctttttgcc cgagttatac gaaaaacaga ataagcagga
     4741 tcggtaacgg gtccgattac gaaacactgc agcaagggaa actcggcaaa gatctcactc
     4801 cacatcgagt ctcatgttga acgctgaaaa gataaatgta tcaaaaggag ccagaaattg
     4861 gctccttttt gattgatgtt cgggcgggta aaatgggtga gttcactgtc aatacattta
     4921 ctggtgacaa gaattatggc aaaggcaaaa cctccttcca ggaaaaataa agatatgacc
     4981 ggagaaaaag agcaccagca cttgatcgag gcaatcctgg atgtagaacc gggtattgtt
     5041 tatatccacg acttacagac aaataagcat gttttcatca accgcgattg gctgaccaac
     5101 gatacataca aaactgctca aaaccaggct gacgaaaacc tcctgcccaa cctcatccat
     5161 cccgaagatc tgccgcgtgt caccgcacat tatgaccagt taagaaccag ctctgacgaa
     5221 caaggtgttc atcagatgga ataccgcgta ctccagaaaa acggggaatg gcgctgggtg
     5281 caaagccggg acacgatatt cagccgtaat gcccagggtg aggtaactca aattctcggc
     5341 atcttatatg acattacaga aagcaagcag gcagatgagc aaatgtattt ccaggcgggt
     5401 cttcttgccc aggtcaatga tgccatcatc gcctctgatg aaaacagccg gatcacaacc
     5461 tggaactcag ccgcggagtt aatgtacgga tggaaagccg atgaggttat cggtcaaatt
     5521 ggcgcggaaa tcgtgaaaac cgagttcaat gggttgaacc gggccgacgt tttgaatatc
     5581 ctagccacag aaggcaaatg gcgtggagaa gtgactcaag cacgcaggga tggctcgcgc
     5641 ttctttgttg agacagcctc cattgttttg agaaatatgc acggagttat aaccggttac
     5701 gcgagtgtaa atcgcgatat caccgcgcgc aggcaagcgg aggaaaaact ggcacggcag
     5761 aacaaacggc tcacagtcct gcgagaaatt gacgctgcca tccttgcggc tgattcgata
     5821 gaacgtatcg tcgatgcggc gttgagccac gtccgccaat tgattgaatg caagcgtgcc
     5881 gccgtcatat tgtttgactg ggagaaacag gaagcggtgt tctataatgt gcagaccgaa
     5941 ggggaaacag ccaatccaaa aggaacgcgg ataccgctgg catcttccca gtggatgatt
     6001 cagatcgttt caaaaaatca gccgcttgtg atcaacgaac taaccatgtt gccagagccc
     6061 attcctcagg tacaacagat gattcaagct ggttttcatt ccatatgcat tctgccgctc
     6121 ttcgtgcaaa acagcctgat cggtgtgttc agtatgagtt ctgaaatgag tggtttcttc
     6181 gattctgaga aaatggatct tggccgtgaa gttgcgaatc aagtgtccat tgcaattacc
     6241 cagagtcgat tggtgagcga attagaagaa cacctgcgcg aacgtgaaat actaattgca
     6301 gatttgaccg ccaagaatac cgagctggaa cgatttattt atacggtttc gcatgaactc
     6361 aaatcgccac tggtcacgat caatggttat ctaggttatc tcgaacagga tgccgtcatt
     6421 ggtaatctgg aacgcatcag agaggataca cagcgtatat cgagtgccgt ggagaatatg
     6481 aaggagctct tgaacgacct gctggacctc tctcgcattg ggcgattaat gaatccgtcg
     6541 cagaaaatcc cttttgaaga ggtggttcgc gacgcactgg aaatcgccca cggtcaattg
     6601 gaggcacata gtatcgccgt tcatagtcag ttagatttgc ccattgtcta cggggaccgc
     6661 ctgcgcctga cagaaatctt gcaaaacttg ctcgacaacg ccgcgaaata catgggcgaa
     6721 caacctgccc cgacgataga gattggtcag caggaagacg ctgaagggca gtttatattc
     6781 ttcgttcggg ataatgggat tggaattgct cctgaatacc atgaacgaat cttcggtcta
     6841 ttcgacaaac tggattcaag gtccgaagga acaggggtgg gccttgctct tgtaaaacgg
     6901 atcatcgaat ttcacggcgg caggatttgg gtcgagtcag agggtgtgcc g
//
LOCUS       DASTJP010000239         8451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1391568, whole genome shotgun sequence.
ACCESSION   DASTJP010000239 DASTJP010000000
VERSION     DASTJP010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391568"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /gene="cyaB"
                     /locus_tag="VFQ23_07215"
     CDS             complement(<1..545)
                     /gene="cyaB"
                     /locus_tag="VFQ23_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HET9906411.1"
                     /translation="MGETNQETEAKFYVQDLNRITARLHALQANLIQPRVFETNIRFD
                     LPDASLRSQRRVLRLRRDTDARMTYKDPSQNEQGVLSRREIEFIVGDFDKAKQFLEAL
                     GYQPVFFYEKYRTTYSLIQTSEVLAHIMVDELPYGNFVEIEGDTVQQIQEIARMLNLK
                     WETAIGKSYNALFEGLCQIRT"
     gene            complement(554..1051)
                     /locus_tag="VFQ23_07220"
     CDS             complement(554..1051)
                     /locus_tag="VFQ23_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9906412.1"
                     /translation="MKYITTVGEKQYLVEIIDDRHVSVDGKVYEVDFESVSGQPVYSL
                     IVDGKSHESYVAQVDDIWQVLMRGRLYPVSVEDEREKRLRAAAGGGVAETGEFHLKAP
                     MPGLVVTIPVAEGQEVKKGQVLLILESMKMQNELKSPRDGKVNRIKVKAGESVEQRQT
                     LLSVM"
     gene            complement(1058..2566)
                     /gene="accC"
                     /locus_tag="VFQ23_07225"
     CDS             complement(1058..2566)
                     /gene="accC"
                     /locus_tag="VFQ23_07225"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET9906413.1"
                     /translation="MFKKVLVANRGEIAVRIIRACRELGIETVAVYSEADRRALHVRY
                     ADEAYLLGPAPSRDSYLRADKILDIAKKSGADAVHPGYGFLAEREDFSAACAERGIAF
                     IGPKPSSIAAMGDKAEARATVIKAGVPVVPGTEDVGNMTDEELLSKAPLIGFPLLIKA
                     TAGGGGKGMREVNSLEEMPTLIQSARREAESAFGEGNVYLEKLVEGARHIEIQILADT
                     HGNVIHLGERECSLQRRHQKLLEEAPSSALDEALREKMGSVAVKAAKAVDYVNAGTIE
                     FLLDKDNNYYFLEMNTRLQVEHPVTEMVTGIDIVKEQIRVARGRSLSYEQHEVKFNGH
                     AIECRINAEDPYNNFLPSTGRITHSLLPTGPGVRVDTGVYPGFEITPFYDPMIAKLIV
                     WGETRAQAILRMRRALEEYRIVGVRTNIPFHQMLMDSHRFMGGQYDTRFVEERFSMEE
                     AEENRENYDDIAAILATLVAHRETELSSHIVQRNERDTSNWKWVGRWERMHR"
     gene            complement(2632..4182)
                     /locus_tag="VFQ23_07230"
     CDS             complement(2632..4182)
                     /locus_tag="VFQ23_07230"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET9906414.1"
                     /translation="MSSQTKIQHLREQKAKSRLGGGAERIEAQHKKGRLTARERIDLL
                     LDKGSFREMDAFVQHRTHDFNLDKQKFMSDSVVTGWGTIEGRLVYVFSQDFTVFGGSL
                     GEVHAEKICKIMDMAMKNGAPMIGLNDSGGARIQEGVVALGGYADIFLRNTMASGVIP
                     QISAIMGPCAGGAVYSPALTDFIFMTRNTSYMFVTGPDVVKTVTHEEVSFEDLGGASV
                     HSEKSGVCHVAAESEADTLYLIRKLLSYLPQNNMEDAPFMPIGDDPLRMDEGLDSIIP
                     DEPNKPYNIKDVIRMIVDGGQFFEIHEGYAQNIVVGFARLGGHSIGIIANQPAVLAGV
                     LDIDASEKGARFVRFCDAFNIPILTFEDVPGFLPGTFQEHHGIIRSGAKLLYAYCEAT
                     VPKLTVITRKAYGGAYCVMSSKHIRSDLNLAWPSAEIAVMGPDGAVNIIFRKELEQAE
                     DPVKKKAELVAEYREKFASPYVAAERGYIDDVIEPKETRPRLINALEMLSNKRDANPA
                     KKHGNIPL"
     gene            complement(4175..4588)
                     /locus_tag="VFQ23_07235"
     CDS             complement(4175..4588)
                     /locus_tag="VFQ23_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906415.1"
                     /translation="MFSQRPFGVTLLLWMVLSLSAWGVIRSLAALRWWDVLYEFDSSL
                     SPLYLLVTGAAWSIAGVVLLWSMWSAKAWSRLAVVAAVLLWLAEYWLERLFFQAPRAN
                     LPFAVALSVLLLAVTLTITSNRHTKDFLTKSEEHE"
     gene            complement(4606..6735)
                     /locus_tag="VFQ23_07240"
     CDS             complement(4606..6735)
                     /locus_tag="VFQ23_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906416.1"
                     /translation="MNTENVKGSRISSRIIKGTSIKDLATRLPWFETMLVIVVMSMSL
                     YAAFSDAQNLSLRWFTRDDAYYYFKVAQNISEGYGSTFDRINPTNGYHPLWMVICIPI
                     FALARFDLVLPLRILLLVMSGLSVATGILLYRLIGKVFAPVIGAIAALFWVFSFDVLG
                     IVYQHGLETGIAAFFVVLLVYKLFDFERSWRAGQVSNKQILTLGIIAALAMFSRLDLV
                     FLAGITGLWIIFRGHPLRYFLPLDIAAIMFSTLMAFVIRAGFPDYYELSTIALAMVVC
                     SLIVKIPLAFFFGLYQRATILNVGELLKQLIFFTISSSAIVGTLILAVSRLGIVESSF
                     PRITILFDFLLTFLLFALSRLIFIGLRIDQKKQGESIERPVSYFLRNWKGWLNESLVY
                     FSVIFGPLAIYMLWNKLAFGTFSPVSGQIKQWWADLPGRAYGGSSRDLMSFLGINYWS
                     DGNAWNPASSLIGWWAENLYRMMILDTWRYLILLTLFALIFFLLLRSSRQKGKRALIQ
                     LAIIPLLSAAWLQVLYYNSSGYASFKEWYWVSQLVLIVLSLSLMLGILYKYLQRFHYA
                     QIALWILAAYMGINMGTSFWKYIRDSMPYNYWSADTSFIDVGPLLEEHTEPGSLIGIT
                     GGGNIGYFIHDRTILNMDGLINSYQYFKALQSGQAGAFLEEIGLDYVLANPVILDQQP
                     YKGQYNEYMDHLNIYYGGKELMRYGAP"
     gene            complement(6732..7778)
                     /locus_tag="VFQ23_07245"
     CDS             complement(6732..7778)
                     /locus_tag="VFQ23_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9906417.1"
                     /translation="MKVVLFRAHGGPEVLEYADFPTPEPKPGEALVRLHAAALNRMDV
                     IVRNGWPGLKLELPHINGADGAGEVVDINSPISNHENAEISVGDRVAINANLGCGECE
                     FCMAGRDNLCKNWHLLGETVRGTYAEFITLPLKQLYRLPDGFEYCQAAAAALVYQTAW
                     HSVVKRGKLQSGETVLVIGAGGGVNTASVQIAKYLGAQVIVVGSDSKKLALAESIGAD
                     ILIDRSKQEDWSRAVFLATNKRGVDVVVDNVGATFMQSLRTLRKGGRLLTVGNSAGPN
                     VEIDNRYMFAKHLSIIGSTMSTLEEFREVMDLIVAGRLKPILDKTYPLKEAALAQDRL
                     WKHQNFGKITLDIL"
     gene            complement(7869..>8451)
                     /locus_tag="VFQ23_07250"
     CDS             complement(7869..>8451)
                     /locus_tag="VFQ23_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906418.1"
                     /translation="AVILFGGILTLNPPANTRLLMTTPPVAIYMALGTYKILEFLKQF
                     RMVPERAAIPAFLVVVLVISYQNIKFYVGDYPRHMYFENANGEFAMETGLMANDLRDI
                     YTLYFLGSPRVFSGFPTIPFLAPDYPRKDLTAADIPTLNLTPNQKVAFFAIPENQPLI
                     EEISQKFPGGERGLIYRKSRPGEILFEYYILAP"
BASE COUNT         2142 a         2210 c         1974 g         2125 t
ORIGIN      
        1 tctgttctga tctgacacaa tccttcaaac agcgcgttat aacttttacc gatagctgtt
       61 tcccatttca gattcagcat cctggcgatc tcttgaattt gttgaacagt gtccccttcg
      121 atttcaacga agtttccata cggtagttca tcgaccatga tatgcgctaa gacttcggaa
      181 gtctgtatga gactatacgt tgtgcgatat ttttcgtaga agaagacggg ttgatatcca
      241 agcgcttcga gaaactgctt ggctttatca aaatctccca caataaattc gatttcccgg
      301 cggctcagca ccccttgttc attttgactc ggatccttat acgtcatacg tgcgtccgtg
      361 tcgcgtctga ggcgcaggac gcgccgttga gagcggagac tggcgtcggg caggtcgaac
      421 cggatgttgg tttcgaacac tctcggctga attagattcg cctgcaatgc atgaaggcgc
      481 gccgttatcc gattcaaatc ctgcacgtaa aactttgctt cggtctcctg attggtttcg
      541 cccataacga aaactacatc acactcaaaa gcgtctgcct ttgttccacg ctctcacccg
      601 ccttcacttt gattcgattc actttgccat cccgtggcga cttaagttcg ttctgcatct
      661 tcatcgattc gaggatcagc aaaacttgtc ctttcttgac ttcctgacct tcggcgacgg
      721 gaattgttac gacaagccct ggcatgggtg ccttgaggtg aaattctcct gtttccgcta
      781 caccaccgcc agccgcggct cgtaatcgtt tctcgcgttc atcctccacc gagacggggt
      841 aaagccgccc gcgcatcaac acctgccaga tgtcatccac ctgtgccaca tacgactcat
      901 gtgattttcc atctacaatc agggaataaa ccggctggcc gctcacggat tcaaaatcga
      961 cttcatatac ctttccatcg acactcacat gccggtcgtc gatgatctcg accaaatatt
     1021 gtttttcgcc aacagtggtt atatatttca tatttggtta ccggtgcatt ctttcccatc
     1081 taccgaccca cttccagtta ctggtgtcgc gttcgttgcg ttggacaata tgagatgaaa
     1141 gttcagtttc gcggtgagca accagagtag ccaggatcgc ggctatgtca tcatagtttt
     1201 ctctgttctc ttctgcttct tccatggaaa atcgttcttc cacaaaacgt gtgtcatatt
     1261 gcccacccat gaagcgatgc gaatccatca gcatttgatg gaaggggata ttggttcgta
     1321 caccgacgat ccgatattcc tccagggctc gcctcatccg caggatggct tgggcgcgcg
     1381 tctcgcccca aacgatgagt ttggcgatca ttgggtcata gaacggcgtg atttcgaagc
     1441 ccgggtatac cccggtatca acacgcacac caggtccggt tggcagcagg ctgtgtgtaa
     1501 tgcgaccggt ggacggcagg aagttgttat agggatcttc cgcgtttatc cgacattcaa
     1561 tggcatgccc attgaacttc acttcgtgtt gttcataact cagggagcgt ccccgcgcca
     1621 cacggatttg ttccttgaca atgtcgatgc cggtgaccat ttctgtaacc ggatgctcca
     1681 cctgcaagcg ggtattcatt tccagaaaat agtagttatt atctttatcc aaaaggaatt
     1741 cgatcgtgcc cgcattgacg taatccactg ccttggcagc tttgaccgcc acgctgccca
     1801 ttttttctct caaggcttca tccagcgcag acgaaggtgc ttcttccagt agtttttggt
     1861 gacgccgctg taaggagcat tcgcgctcac cgagatggat gacgttccca tgtgtatctg
     1921 ccaggatttg gatttcgata tggcgcgcgc cctcgacgag tttttccaga taaacatttc
     1981 cctctccgaa cgcagattcc gcctcacgcc gcgcggactg tatcagggtt ggcatttcct
     2041 ccaggctatt tacctccctc atgcctttgc cgccgccgcc tgctgtggct ttgatcaaca
     2101 acgggaaccc aattaaaggc gccttgctta acagttcttc atcggtcata ttgcccacat
     2161 cttccgtgcc gggtaccaca ggcacgcctg ccttaatgac cgttgcacgc gcttccgcct
     2221 tatctcccat cgctgcaatg gacgatggct tgggaccgat gaaggcaatg cctctctctg
     2281 cgcaggctgc tgaaaaatct tcgcgttccg ccagaaagcc atacccggga tgaacagcgt
     2341 ctgcaccgga ttttttagca atatccagga ttttatccgc acgcaagtat gagtcgcgcg
     2401 aaggggcagg tcctaacaaa taggcttcat cggcatatcg cacatgcagt gcacgccgat
     2461 ccgcttcgga ataaacggcg acggtttcaa tgcccaattc acgacaagcg cgaatgatgc
     2521 gaacagcgat ctctccacga ttagcgacca agactttttt aaacatacgc aatccttgta
     2581 ggggcggggt cgtcccgccc agggcgagag aacctcgccc ctacacaata tttataatgg
     2641 aatattcccg tgcttcttcg cggggttggc atcgcgcttg ttgcttaaca tttccaaagc
     2701 gttgattaaa cgcggacgag tctctttcgg ttcgatcacg tcgtcaatat atccgcgttc
     2761 tgccgcgacg tacggactgg cgaacttctc gcgatactca gccaccaatt cagctttttt
     2821 cttgaccgga tcttcagctt gctcaagttc cttgcggaag ataatattga cggccccgtc
     2881 cggccccatg accgcgatct ccgcggaggg ccaggcgagg ttcaagtcac tacggatgtg
     2941 cttggaggac atcacacaat aggcgccccc ataagccttg cgtgtaatca ccgtcagctt
     3001 tgggaccgtt gcctcacaat aggcatagag caatttagcg cccgaacgaa taatgccatg
     3061 atgttcctga aatgtccccg gcaaaaagcc tggcacatct tcaaaggtga ggatagggat
     3121 gttgaaggca tcacaaaagc gtacaaaacg tgcgcctttc tcgctggcgt caatatctaa
     3181 aactcctgca agcacagcgg gctgatttgc aataattcct atcgaatgac caccgagacg
     3241 tgcgaatcca acgacaatat tctgagcata tccctcgtgg atttcaaaaa attgaccacc
     3301 atccacgatc attcgaatga catctttaat attgtagggt ttgttcggtt catccgggat
     3361 aatggagtct agtccttcgt ccatacgaag cgggtcgtcg cctatcggca tgaacggtgc
     3421 atcttccatg ttgttttgag gcagatagct caacagcttg cgaatcagat aaagtgtgtc
     3481 ggcttcactt tcggcagcca catgacacac gcccgatttt tccgagtgga cactcgcgcc
     3541 gcccaaatct tcaaacgaga cctcttcatg cgtcaccgtt ttgaccacat ctggtccggt
     3601 gacgaacata tacgatgtgt tgcgcgtcat gaagatgaaa tcagtgaggg caggggagta
     3661 gaccgcaccc ccggcgcagg gtcccataat ggcagagatt tgcggtatca caccggaagc
     3721 catcgtattg cgcaggaaga tatctgcgta gccgccgagc gccaccacac cttcctggat
     3781 tcgtgcccca cctgaatcat tgaggccgat catgggggcg ccgtttttca tcgccatatc
     3841 catgattttg cagatcttct ccgcatggac ttcgccgagg ctcccgccga aaactgtaaa
     3901 atcctgagag aaaacgtaaa cgagacggcc ttcaattgtg ccccagcccg tcaccaccga
     3961 atcgctcata aacttttgct tatcaaggtt gaagtcgtgt gtacggtgtt gcacgaaggc
     4021 gtccatttcc cggaatgaac ctttatcaag caagaggtcg atccgctcgc gcgccgtgag
     4081 cctccctttt ttgtgttgag cctcgatacg ctcggcgcca ccgcccagac gtgattttgc
     4141 cttttgttca cgcagatgct gaattttcgt ttgactactc atgttcctcg cttttcgtaa
     4201 gaaagtcttt tgtatgtcga tttgaagtga tcgtaagtgt caccgctaac agcaaaacag
     4261 ataacgcaac tgcaaagggg agattcgctc ttggagcctg gaagaataac cgctcgagcc
     4321 agtattctgc gagccataac agcacggccg caacgaccgc caggcgtgac caggccttag
     4381 cactccacat gctccagagc aaaacgactc cggcgatgct ccaggctgcc cccgtcacca
     4441 ggaggtacag cgggctcagg cttgagtcaa attcatagag tacatcccac caacgcaggg
     4501 ccgcaagaga gcgaatcaca ccccacgccg ataggcttaa caccatccac aggagcaggg
     4561 ttacaccgaa aggtcgctgt gagaacatga tttagtctct atttgtcaag gcgctccata
     4621 gcgcattaat tcttttcccc cgtaataaat atttagatga tccatatatt cattgtattg
     4681 acccttgtag ggctgttgat caaggattac tggattcgcg agcacatagt ctagcccaat
     4741 ttcttcaaga aatgccccgg cttgcccgga ctgcaacgcc ttgaaatatt gatagctatt
     4801 gatgagaccg tccatattga ggatagtacg atcgtgaata aaatagccga tattgcctcc
     4861 acccgtgatc ccaatcaggc tgccgggctc ggtatgttct tcgagaagag ggccgacgtc
     4921 aataaacgat gtgtctgctg accaataatt atagggcatt gaatctcgga tatatttcca
     4981 aaacgaggtg cccatattga ttcccatata ggcagccagt atccacaagg cgatctgagc
     5041 gtagtgaaag cgttgaaggt atttatataa tattcccagc atcaaactca gacttaatac
     5101 gataagaacc aactggctga cccagtacca ttccttgaag gatgcatatc ctgaggaatt
     5161 gtaataaagc acttgcagcc aggctgcact caaaagaggg atgatcgcca attgaatgag
     5221 ggctcgtttt cctttctgtc ggctggaacg tagaagtaga aaaaaaatca gggcaaataa
     5281 ggtaagaagt atcaaataac gccatgtgtc gaggatcatc attcgataca gattctcagc
     5341 ccaccaaccg atgaggctcg aggcaggatt ccaggcgttc ccatcgctcc aatagttgat
     5401 tcccagaaag gacataaggt cccgtgacga tccgccatac gctcgacctg ggaggtctgc
     5461 ccaccattgt ttgatctgcc cgctgacggg cgaaaaagtt ccaaaggcta gtttgttcca
     5521 cagcatgtaa attgccaatg gaccaaaaat gacactgaaa taaacgaggc tttcgttaag
     5581 ccatcctttc caatttcgga ggaagtaaga caccggtcgt tcaatcgatt cgccttgttt
     5641 tttctgatct atgcgaagac cgatgaagat caatctgctc agcgcgaaaa ggaggaaggt
     5701 aaggaggaag tcgaacagga tggtgatgcg cggaaacgaa ctctctacga tgccaagcct
     5761 tgagactgcc agaatcagtg tgccaacgat ggctgaactg gaaatcgtaa agaaaatcag
     5821 ttgcttaagg agttctccca cattcaaaat ggtagcgcgt tgatatagcc caaagaaaaa
     5881 ggccagtgga attttgacga tcaaactgca aactaccata gccaaagcaa ttgtggatag
     5941 ctcgtaatag tcagggaatc cggcccgaat tacaaatgcc attaacgtag aaaacataat
     6001 ggcagcaata tccagtggaa ggaaatagcg cagaggatgc ccgcgaaaaa tgatccacaa
     6061 gcctgtgatc cctgctaaaa agacgaggtc cagcctgctg aacatggcca gcgccgcgat
     6121 aattccaaga gtcagaattt gtttgttcga aacttggcct gcccgccatg atctctcaaa
     6181 atcaaacaac ttataaacca gtaaaacgac gaagaatgct gcgatgcctg tttcgagtcc
     6241 atgctgatag acaattccca aaacatcaaa actgaagacc cagaacaaag cagcaattgc
     6301 tccaatcacc ggtgcgaaga cttttccaat taatctatat aacagtatgc ccgtcgccac
     6361 cgatagccca ctcataacta acaacaaaat gcgcaatggt agaaccagat caaagcgtgc
     6421 cagcgcgaaa atgggaatac agatcaccat ccataacggg tgatagccat tcgtgggatt
     6481 gatccggtcg aacgtactcc catatccctc gctgatgttc tgtgctactt tgaaataata
     6541 ataggcatcg tcacgggtaa accagcgcag agataaattt tgtgcgtcag agaaggctgc
     6601 atacaggctc atcgacatga cgacaataac taacattgtt tcaaaccagg gaagccgggt
     6661 tgccaaatcc tttatgcttg ttcctttaat gatccgggag gagatacgcg aacctttaac
     6721 gttttcagtg ttcataaaat atctaatgta atttttccaa aattttggtg cttccacaaa
     6781 cggtcctggg ctaatgcagc ctctttaagt ggataggtct tatccaggat tggtttaagc
     6841 ctgccagcga caatcagatc catgacttcg cgaaattctt caagtgtgct catggtagag
     6901 ccaatgatac tcaaatgttt tgcaaacata tagcgattat caatctcaac gtttggtcct
     6961 gcgctgttcc caacggtcag tagtcttcca cctttccgta acgttcgcag actttgcata
     7021 aacgtggcgc caacgttatc tacaacgaca tctacaccgc gcttgtttgt cgccaggaaa
     7081 actgccctgg accaatcctc ttgctttgac ctgtcaatca ggatgtctgc gccaatggac
     7141 tcagccaatg ccagcttctt cgaatcggat cccaccacaa taacctgtgc tccgagatat
     7201 ttggcgattt gaacactggc agtatttacc ccgcctcctg ccccgatgac cagaaccgtc
     7261 tcgccgcttt gcaatttgcc gcgtttgaca accgagtgcc aggccgtctg ataaaccaat
     7321 gcggccgcgg cagcttgaca atactcaaac ccatctggca ggcgatacag ctgcttgagg
     7381 ggtagcgtta taaattctgc ataggtccct cgtacagttt caccgagtaa atgccagttt
     7441 ttgcagagat tatctcttcc tgccatgcag aattcgcatt ccccacaccc taaattagcg
     7501 ttgatggcga cacgatcacc gacgctaatc tctgcgttct catggttgga gattggggaa
     7561 ttaatgtcga ccacttctcc agcgccatct gcaccgttga tgtgaggtag ctccagcttc
     7621 aatcccggcc agccattgcg cacgatcacg tccatgcggt tgagcgccgc ggcgtgtagt
     7681 cggaccagag cctcacctgg ttttggttcc ggcgtaggga aatctgcgta ttccaaaact
     7741 tcaggcccgc catgagcccg aaacaggaca accttcatgc ggatgattgt aactgattag
     7801 gggtgattct gtttatgtgg aagaaccgct gtaatccagc ggcgaattaa tgcttgggtt
     7861 ctgaaatgtc agggtgcaag aatgtaatat tcgaacaaaa tctcgcccgg cctggacttg
     7921 cggtagatca aacctctttc accaccggga aacttctggc taatttcctc gatcagaggt
     7981 tgattctctg gtattgcaaa aaaggcaact ttttgattcg gcgtcaggtt gagtgttggg
     8041 atgtctgctg cagtgaggtc ttttcgcggg taatctggtg caagaaaagg gatagtgggg
     8101 aagcctgaaa aaacacgcgg cgagcctaag aagtacaacg tatagatatc ccgtaggtca
     8161 tttgccatga gtcctgtttc catggcaaac tcaccatttg cattttcaaa atacatatgg
     8221 cgcgggtaat cccccacata aaattttatg ttttgatacg atatgaccaa aaccacaacg
     8281 agaaaggcag ggattgccgc acgctccgga accatccgga actgttttaa aaattctaaa
     8341 attttatatg tacctaatgc catatatatc gctactggtg gggttgtcat cagcaggcgc
     8401 gtgttggcag gtgggttgag cgtgagaatt cccccaaaca ggatgacggc c
//
LOCUS       DASTJP010000240         8994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_535499, whole genome shotgun sequence.
ACCESSION   DASTJP010000240 DASTJP010000000
VERSION     DASTJP010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535499"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFQ23_07255"
     CDS             <1..682
                     /locus_tag="VFQ23_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET9906419.1"
                     /translation="TSTAEMRTDELMRAAKQLGLKDVFFLGYRDSGMPGTEENKHPDA
                     QINHSIDEVAGRVVKIIRAVKPDVVLTHDPIGGYKHPDHIHIHKATKLAFEKADDPAF
                     HPEAGLPFKPRALYYQVFSRTLLRVMVRVMRLFGKDPTKFGRNSDVNLLELAKVDFPI
                     HVRLNTRSVQDIKIKASAQHASQGGTQIRRGWMGLISRILGEREEFMRAYPPVADGKY
                     KVSDDLFV"
     gene            695..1357
                     /locus_tag="VFQ23_07260"
     CDS             695..1357
                     /locus_tag="VFQ23_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9906420.1"
                     /translation="MIEQTERVQGPHRSQSIRMKGEPLDRAQAAMIMIHGRGASAEDI
                     LSLSNEFDQPGFAYLAPQAAGNTWYPNRFLVPLDQNEPWLSSALSFVGDIYDQITGEG
                     IPNERIMLLGFSQGACLTLEFAARNARRFGGVVGLSGSLIGPDDTPRDYQGSLEGTPV
                     FLGCSDVDFHVPKERVSQSAEVLQNLGGNVTKRLYPNMDHSVNQDEIDFVRGMMHSVL
                     SL"
     gene            1394..2518
                     /locus_tag="VFQ23_07265"
     CDS             1394..2518
                     /locus_tag="VFQ23_07265"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET9906421.1"
                     /translation="MHEFNYNSYAQEVIFGSGSLRRLSEWVDRAGVHKMMLCANPSMQ
                     VGGKITLVENILEKRLAVVFDRVQPHVQDLQVDEIVAVAVAKDVDAFVALGGGSSVGI
                     AKAASAACSKKMSRDYPVIAIPTTYAGSEMTPVYGITRTQEDPPRKVTSNDPKAIPRL
                     VIYDPNLTLDLPPAITASTGINALAHCIEALYSIRKNPLSTAAATAGVKHIFNALLRC
                     YTHPDDLNARSEMMLGSHLAGLSLASVTMGLHHGLCHVLGGTANVPHGIANSILLPHV
                     IRFNADVTAKQLLAVAAEMEIPINGISPIIAIEAMVQKLFHFIGQMNLPQHLRDSGVG
                     LRQSDLPLLAELAFQNQTVQHNPKPITNVFQIAELLHDAW"
     gene            2548..3105
                     /locus_tag="VFQ23_07270"
     CDS             2548..3105
                     /locus_tag="VFQ23_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9906422.1"
                     /translation="MTVFQTQGVHHITFVGSNRQAIIDFYQGVLGMPLVFEQPNLDVP
                     DELHLYFDPGDGRLITFFVRPTRVNDPTPNPQEIGNLHHIAYTVSRATYTQIAARLKE
                     QNIPNTGPIDRGFMDSIYFRDPNGQLLEMACFKFLPPEGFTTADVLSVAHRLRVERGD
                     YAIADQHLADAIAELTTRKLSVASG"
     gene            3205..3564
                     /locus_tag="VFQ23_07275"
     CDS             3205..3564
                     /locus_tag="VFQ23_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9906423.1"
                     /translation="MSKRNVVHIEIPAENSQAAGNFYEELFGWKMQHVPEFDYTMWDD
                     GAGGGGGFNKVSDQTPVGQVLVYIHSDDIDADLKKIEALGGTIITPKTEIPGTGWYGW
                     FKDPTGNTLALYTSINS"
     gene            complement(3645..4619)
                     /locus_tag="VFQ23_07280"
     CDS             complement(3645..4619)
                     /locus_tag="VFQ23_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906424.1"
                     /translation="MRSSQFYLKRKLYNCSLLKDKQGETLMTNILETLNQHKQKLNLP
                     KGSSRITADSTVPAKTNLFTLVTAGIFLAVFGLVAQFANPLISGVTSSAAALTIITAG
                     AVGIERTLETFWTYMGLTRGSWWPLGPVREQLDGLLSSLDTSFEPFYEEAQLKIQELQ
                     QTTKWGRQKIDAAAKDLEEIKKHVDQLKKLPLDSQRVQLITASASRSIEYFSTKYPEI
                     RDTARLANFGIQGVSDFVATFKDNPGRRLISLFAGAIAGLIIAGVLRLDLFQAVLQTS
                     SLAQESGFLPYTGVAITGLLMGLGANPTHEAIRLLQEIKNRRKSENVK"
     gene            4647..7610
                     /locus_tag="VFQ23_07285"
     CDS             4647..7610
                     /locus_tag="VFQ23_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9906425.1"
                     /translation="MLQFPEYINELRKDFRGEIRNDLASRILYSTDASIYHVEPLGVA
                     IPKTQEDLHTAVELAAKYKIPILPRGSGTSLAGQAVGEALILDCSRWLDKIIEINPEE
                     HYAIVEPGVVLSNLNKAAGKYGLMFGPDPASAERATMGGVIGNNATGAHSILYGMTAD
                     HLISADVIMADGSLATLGERSTLDSHLVSNLYSSALKIRQKSVDAITSHWPRTWRNSA
                     GYRLNYLVPWSPSRPSQWIGEYPANLEPGTWNLAHLLAGSEGTLAVIRRAKVNLVPKP
                     KYTILSVLSYASIADACDDVPRLLAHKPSAIELIPQIILRNARSIPVYARQMGWLFPK
                     GDAIADPEALLVVEFSGDPPQMLKDRARKIGDIVTIAESVEDQARIWNIRKVGLGLLD
                     SRPGAARPIAFIEDCAIPVDHLGEFVREVEAILLAHKTTGGIYAHASGGCLHIRPILD
                     LQAESGLNSLRSIAEATLALTLRLGGSMSSEHGDGLARGEWLQKTYGDELIEAMRMLK
                     QSADPNNLLNPNKLFDAPKMDSHLRHEKNAVTQSWSPALDFSRSGSFVTAVAQCNGQG
                     LCRKNTGVMCPSFQATRDEMHSTRGRANLLRALISRDSLQLPNSPSQNIKMVEATARA
                     LDLCLACKGCAAECPSGVDMAKLKYEFENEYYKTRRRPFRDYLFGYFHVTAGLASSVA
                     PVANALLNVSVVRYLIAKLLGITPHRPFPKFANPNPVAARPQGGREKVIFLSDPFSLY
                     IEPEVQQAALEVLHHCGYDVYVLPVIGAGASLLSKGFVHAAKQHSRKVLAELNKIDPV
                     HDAVIVGVEPPEVYLLKHDYFDLLPEFRDEISRRAEKTWLLDEFLLRTENFEALRVGN
                     KARFSSTENSPLSKLYFQPHCHQRAEGLSADGKPSGATATVELLQACGYDVELMDTGC
                     CGMAGSFGYDAEHYDLSMKIGELKLFPKIKELEIENRAIVSTGAACRMQIRQGTGVQA
                     QHSILLVRAALETRN"
     gene            complement(7629..>8994)
                     /locus_tag="VFQ23_07290"
     CDS             complement(7629..>8994)
                     /locus_tag="VFQ23_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906426.1"
                     /translation="TFPLPSRTYTNVTSDMPNQNYTPGFNPSSGCAEVAVTIANNNLT
                     TFALPPGRSMREGYAGVNNGPARINSTNIVNILAALRVIWKEPGSRFSYSEMIGLPDE
                     QLSSEYWFPWYNNAATNSMDQGLRIANVNTVADNTVEVWVGATTKLDTITLGAGESVR
                     VNYNVDNGPLRVFCTTCSNTLYDQVITTLRVIWKEPGFRASYSEMLGLPSGQLSDEYW
                     FPWYNNAVPTSMDQGFRIANISASEANTLEVRLGNTLLDTINLGARESIRVGYNVDNG
                     PIRIVCTTCSSAVDDKILAALRVIWKEPGFRSSYSEMMGLPKEQLSNEYWFPWYNNAA
                     TNSMDQGFRIANVDLNASNNVEVWVGSTKLQTISLAAGGSTRVGYNVDNGPVHIVCTT
                     CGTDNDKIIAALRVIWTESGFRSSYSEMMGLPAEQLSQQYWFPWYNNADTNSMDQGFR
                     IAVP"
BASE COUNT         2218 a         2120 c         2310 g         2346 t
ORIGIN      
        1 tacatccacc gctgaaatgc gaaccgatga attgatgcgc gcagcaaagc aactcggtct
       61 gaaggatgta ttttttctcg gctaccgcga ttcaggaatg cctggtacag aagagaacaa
      121 acaccctgat gcgcagatca atcattcgat cgatgaagta gccgggcgtg tggtgaagat
      181 tattcgtgcg gtcaaaccag atgtggtcct gacccatgat ccgatcggcg gatataaaca
      241 tcccgatcac attcatatcc ataaggcaac caaacttgcc tttgaaaaag cggatgatcc
      301 cgcgtttcat cctgaagccg gattgccctt caaacctcgc gcactttact atcaggtgtt
      361 ttcacgtacc ttgttgagag ttatggtgcg agtgatgcgc cttttcggta aagacccaac
      421 aaagttcggc cggaatagtg atgtcaattt gcttgagctg gctaaggtgg acttcccgat
      481 tcatgtgcgc ttgaatactc gctccgttca ggatatcaag attaaggcaa gcgcacagca
      541 tgcctcgcag ggagggacgc aaattcgccg tggctggatg ggcctgattt caaggatcct
      601 cggcgagcga gaggagttta tgcgtgcata tcctccggtt gcagatggaa aatataaagt
      661 ctctgatgac ttatttgtgt aaagagagaa tactatgatt gaacagactg aacgtgttca
      721 ggggcctcat cgaagccaat caattcggat gaagggtgag ccgctcgatc gggcgcaggc
      781 ggcgatgatc atgatacacg gacgtggtgc atcagccgaa gacatattgt cactgtctaa
      841 cgaattcgat caaccagggt ttgcttatct ggcgccgcag gccgcaggca atacctggta
      901 cccgaatcgc tttcttgtcc cactggacca aaatgaaccg tggctttctt ctgccttatc
      961 ttttgtgggc gatatttatg atcaaatcac tggggagggg attccgaatg aacgcatcat
     1021 gcttctgggc ttctcgcagg gggcgtgcct cactcttgag tttgcagcac gcaacgcgcg
     1081 tcgatttgga ggggtagtag gactcagtgg ctcattgatc ggtccggatg atacgccccg
     1141 tgactaccag ggctcattgg aaggaacccc ggtctttctc ggttgtagcg atgtggattt
     1201 tcatgttcca aaggaacgag ttagccaatc agccgaagtt ctgcagaatc ttggcggaaa
     1261 tgtgactaaa cgattgtatc caaacatgga tcacagtgtt aatcaggacg agattgattt
     1321 tgtgcgcggt atgatgcatt cagtattaag tttgtaaagt tttttgctgg gttgaacgga
     1381 ggccagcggt tcgatgcatg agttcaacta taactcgtat gcgcaagagg tgatctttgg
     1441 gtcaggctca ttacggcgtt tgagcgaatg ggtcgatcga gcgggcgttc acaagatgat
     1501 gctgtgcgcc aatccttcta tgcaggtggg cgggaaaatc acattggttg aaaatattct
     1561 ggagaaacgt ctggcagtcg tatttgatcg tgttcagcct catgttcagg atctccaggt
     1621 ggatgaaatt gttgccgtgg ctgttgcaaa agatgtagat gcctttgtgg cactgggggg
     1681 tggcagttca gttggaatag cgaaggccgc ctccgctgcg tgctcaaaga agatgagcag
     1741 agactatcct gtgatagcga ttcccactac ctatgccggg tctgaaatga cccccgttta
     1801 cggcatcacg cgaacacaag aagacccgcc ccgcaaagtc acatccaacg atcccaaagc
     1861 gattccgcgg ctggtgattt atgatccgaa cctgaccctc gacttgcctc ccgccattac
     1921 cgcttccaca ggcattaatg cgctcgctca ttgcattgaa gcactttact cgatacgaaa
     1981 aaatccattg tcgactgccg cagcaactgc cggcgtaaaa cacattttca atgccctctt
     2041 gcgctgttac acccatccgg atgatttgaa tgcccgctct gaaatgatgc tgggctcgca
     2101 tctcgccgga ctgtcgctgg cttcggtcac gatgggcctt catcatggtt tatgtcatgt
     2161 gttgggtggc acagcaaacg tgcctcacgg aatcgcgaac agcatacttt tacctcacgt
     2221 catccgtttc aatgcggatg tgactgcgaa acagttattg gcggttgccg cggagatgga
     2281 aattcccatc aatggaatca gtccgattat cgcgattgaa gccatggtgc aaaagttatt
     2341 tcatttcatt ggtcagatga acctacctca acatttgcgc gattcaggag tcgggctgcg
     2401 gcaatccgat ctgccactgc tagccgaact ggcttttcaa aatcagactg tgcagcataa
     2461 ccccaaacca attacgaacg ttttccaaat tgccgagctt ttacacgatg cttggtgaga
     2521 atatcaatta cttcgggagc agaagctatg actgtatttc aaacacaggg agtccaccat
     2581 attaccttcg ttggttcgaa tcgccaggcg atcatcgatt tttatcaggg cgtattgggt
     2641 atgccgctgg tattcgaaca gccaaacctc gacgtcccgg atgaactgca cctttatttt
     2701 gatccgggtg atggcaggct gatcaccttt tttgtgcggc ccacgcgcgt gaatgaccca
     2761 acgccgaatc cgcaggagat tggcaacttg catcatatcg cctacaccgt ctcgcgtgcc
     2821 acgtacaccc agatcgcggc gcgcttaaaa gaacagaata ttcccaacac cggcccaatc
     2881 gaccgtggat ttatggactc catttacttc cgtgacccga atggccaact gctcgaaatg
     2941 gcttgcttta agtttcttcc accagaggga tttactacgg cggacgtcct gagcgtggcg
     3001 catcgtttac gcgttgaacg aggggattac gcgatcgctg atcaacacct cgctgacgca
     3061 attgcagagc tgacaacacg aaaactttcc gttgcatccg gataatttca aaaagtagtt
     3121 cctgagaggg cttgtgattg tagaatgaat gttctataat tgattaacta attcattacg
     3181 cagacacaaa ccagaggagc taaaatgtcc aaacgcaacg ttgtccatat tgagattccc
     3241 gcggagaatt ctcaagccgc tgggaatttc tacgaagaat tgttcggctg gaagatgcaa
     3301 catgtccccg aattcgacta caccatgtgg gatgatggcg ccggtggcgg tgggggattc
     3361 aataaggttt ctgatcagac tcctgtcgga caggtgctgg tctacatcca tagcgatgac
     3421 atcgatgcag atttgaaaaa gatcgaagcg ctaggcggga ctattatcac gccgaagacg
     3481 gaaattccag gtaccggctg gtatggttgg ttcaaagacc caacggggaa cacactggct
     3541 ttgtatacca gcataaattc ctagttcaac aagtaaattt ttcagatcta aataatattg
     3601 ggataggctt agagcctatc cctgttttgt gtttagaccg attctcactt aacattttcg
     3661 gatttccgtc tattcttgat ctcttgcagc aatcgaatgg cttcatgtgt tggatttgca
     3721 cccagcccca tgagtaaacc tgtaatagca actccagtgt aaggtaggaa accggattct
     3781 tgcgcgaggc tggacgtttg caagaccgct tgaaacaggt ctagcctaag aacacctgca
     3841 attatgagac cagcaattgc gcccgcgaag aggctgatca accgtctgcc agggttatcc
     3901 ttgaatgttg cgacaaagtc tgaaactccc tggattccaa agtttgcaag tctggcagta
     3961 tctcgaatct ctggatactt tgtagaaaaa tactcaatgc ttcgggatgc tgatgcagta
     4021 attagctgta cacgttgact atctaaggga agttttttta gctgatcaac atgtttcttg
     4081 atctcctcga ggtcttttgc tgctgcatct attttttgcc gtccccattt tgtagtctgc
     4141 tgaagctctt gaatctttag ctgagcttcc tcgtaaaacg gttcgaagga ggtatctaag
     4201 ctcgagagaa gcccatctag ttgttcacga actggtccca atggccacca agagcctcgt
     4261 gtcaacccca tataagtcca aaaggtctcc aatgtacgct cgatcccaac cgctccagca
     4321 gtgataatgg tcaaagccgc cgctgaactt gtaactccag aaataagtgg attggcaaat
     4381 tgagccacga gaccgaaaac tgccagaaag atgcctgcag taaccaaagt aaacaagttg
     4441 gtcttagcag gaacagtgga atctgcagtg attctgctac ttccttttgg aagattcaat
     4501 ttttgcttgt gttgatttag ggtttccagt atgtttgtca taagagtttc tccttgtttg
     4561 tctttgagaa gcgaacaatt ataaagtttg cgtttcagat aaaactgact tgatctcatg
     4621 cggcggagca gcgttagaat tagtgcatgt tacagtttcc tgaatatata aacgaacttc
     4681 gcaaagattt tcggggagaa attcgaaacg acctcgcctc ccgcattctt tacagtaccg
     4741 atgcgtccat ttatcacgtt gagccacttg gtgtagccat tccaaaaacg caggaagatc
     4801 tccataccgc ggttgaactc gccgcgaaat acaaaatccc tattttgcca cgtggttcgg
     4861 ggacttctct agccgggcag gcagttgggg aagcgctgat tttggattgt tcgcgctggc
     4921 tggataaaat tattgagatc aatccagaag aacattacgc aatcgtcgag cctggcgttg
     4981 tgctctcaaa tctcaataaa gctgcgggga aatatggcct gatgtttggc ccagaccctg
     5041 cctcggcgga acgcgcgacc atgggtggag tgatcggtaa caatgccacc ggagcgcact
     5101 cgattctcta tggtatgaca gccgatcatt tgatctccgc cgatgtgatc atggcggatg
     5161 gcagtctcgc cacattggga gaaagaagta cgctcgatag tcatctcgtt tccaacttat
     5221 attcctccgc attgaaaatt cgtcaaaaat ctgtggatgc gataactagt cattggcccc
     5281 gaacgtggcg taactccgcg ggttatcgat tgaattatct agtgccatgg tcgccttcga
     5341 ggccttcgca atggattgga gaatatcctg ctaacctgga acctgggacc tggaacctgg
     5401 cacatttatt ggcggggagt gagggcacac tggctgtgat ccgccgcgca aaagtgaatc
     5461 tggttccaaa gccaaagtac acgattcttt cggtgctttc ttacgcgagc atcgcggatg
     5521 cgtgtgatga tgtgccgcgt ctgttggcgc acaaaccttc tgcaatcgaa ctgattccgc
     5581 agattatttt gagaaacgcg cgcagtattc cagtgtatgc gcgtcaaatg gggtggttgt
     5641 ttcccaaagg ggatgctata gccgacccgg aagcgttgct tgtcgtggag tttagcggcg
     5701 acccacctca gatgttgaaa gatagggcgc gaaaaattgg tgatatcgtg accattgcag
     5761 agtctgtcga agatcaggcg cgcatatgga atatccgcaa agttggactc ggacttctcg
     5821 attcccgacc aggtgcagct cgtccaattg cgtttatcga agattgtgct atccctgtgg
     5881 accatctggg ggaatttgtg cgtgaggtcg aagctatcct gttggcgcac aaaacaacag
     5941 gagggattta tgcacatgct tccggtggat gcctgcacat tcgtccgatc ttggatctgc
     6001 aggctgaatc gggacttaat tctttacgat cgatcgctga agcgactctg gctttgacgt
     6061 tgcgacttgg cgggtcgatg agcagtgaac acggggatgg gcttgcgcgt ggggagtggc
     6121 tgcaaaagac atacggtgat gaattgatag aagccatgcg catgttgaag cagtccgctg
     6181 acccaaataa cctgcttaac ccaaataaac tgttcgacgc gccaaagatg gattcgcact
     6241 tacgccatga gaaaaatgct gtcacacaat cctggtctcc agcactggat ttctctcgtt
     6301 ctggaagttt tgtaaccgct gtggcgcaat gtaatggtca ggggttatgc aggaaaaata
     6361 ctggtgtgat gtgcccgtcc tttcaggcga caagagatga aatgcattcc actcgcggaa
     6421 gagccaattt gctgcgggct ttaatttcgc gggattcttt gcaactcccg aatagcccgt
     6481 cacagaatat caaaatggtt gaggcgacag cccgcgccct ggatctttgt ctcgcctgta
     6541 agggctgcgc agccgaatgc ccatctggcg tggatatggc aaagttgaag tatgaatttg
     6601 aaaacgaata ctataagact cgccgaagac cgttccgcga ttatctattt ggttactttc
     6661 atgtgaccgc tgggcttgct tcgtcagttg ctccggtagc gaacgcgttg ttgaatgtat
     6721 ccgtggtcag gtatcttata gcgaagttac ttggtattac cccccatcgt ccttttccaa
     6781 aatttgcaaa cccaaatcct gtagccgccc ggccacaagg gggtagagaa aaagttatct
     6841 ttctttccga tcctttttct ctatatatcg aaccggaagt acaacaagct gcattagaag
     6901 tcctgcatca ctgtggatat gatgtttacg tattgccagt gatcggcgca ggggcctctt
     6961 tactctctaa aggatttgtc cacgctgcca aacagcattc ccgcaaagtg cttgccgaat
     7021 tgaacaaaat cgatcctgta cacgatgcag tgattgttgg ggtagagcct ccggaagttt
     7081 atttgctaaa gcatgattat tttgacctgc tacctgaatt tcgggatgaa atctctcggc
     7141 gcgctgaaaa gacgtggctg ttggatgagt ttcttttacg taccgagaat ttcgaagctt
     7201 tgcgcgtagg caataaggcg cggttttcca gcactgaaaa tagtcctcta tcaaagctat
     7261 attttcagcc acattgtcac cagcgcgccg aaggtctctc ggcggatggt aaacctagtg
     7321 gggcgactgc aacagtggag ctattacaag cctgtggata cgacgtggag ttgatggaca
     7381 caggttgctg tggaatggct ggctcgttcg gttacgacgc tgagcattat gatctttcta
     7441 tgaaaattgg agaactgaag ctatttccaa agattaaaga attggaaatc gaaaatcggg
     7501 caattgtaag taccggtgca gcttgccgga tgcagattcg gcagggaaca ggcgttcagg
     7561 ctcagcactc aatcttattg gtcagagcag cgttagaaac gcggaattaa ttcggaaata
     7621 ttcacttgtt agggcaccgc gattcgaaac ccttgatcca tggagtttgt atccgcattg
     7681 ttgtaccagg ggaaccaata ttgttgcgag agttgttcgg ctggtaatcc catcatttca
     7741 gaataactgc tgcggaagcc agactccgtc caaatgactc gtaaagcggc tatgatctta
     7801 tcgttgtcgg tgccgcaagt ggtgcagacg atgtggacgg gaccattatc cacgttgtag
     7861 cctactcggg tgctgcctcc ggctgccaga gaaatcgttt gaagctttgt gctacccacc
     7921 cagacttcga cgttgttgct cgcattgagg tctacgttag caattcgaaa gccttgatcc
     7981 attgaatttg ttgcggcgtt gttataccag ggaaaccaat actcattcga aagctgctct
     8041 ttgggcaacc ccatcatttc cgaatagctg gaacgaaagc ctggctcttt ccagatgacg
     8101 cgcagggctg cgaggatctt gtcgtccacg gcggatgagc aggtggtaca aacgatccgg
     8161 atcggtccat tgtccacgtt gtaaccgact cgaatgcttt cccgtgcgcc aagattgatc
     8221 gtatccagca gagtattgcc caatcgcact tctaaggtgt ttgcttcaga ggcggaaatg
     8281 ttcgcgattc gaaagccctg atccatggaa gtaggaaccg cattgttgta ccagggaaac
     8341 caatattcat ccgagagctg gccggatggc aaccccagca tttctgagta gctggcgcgg
     8401 aagcctggtt ccttccagat tactcgcagt gttgtgatga cttggtcgta gagcgtgttc
     8461 gaacaggtgg tgcaaaaaac gcggagaggg ccgttatcta cgttatagtt gactcttaca
     8521 ctttcacccg cccctagcgt aatcgtgtcc agcttcgtcg tggcccccac ccagacttca
     8581 acggtattat cagcgaccgt gtttacatta gcaatgcgca gaccctgatc cattgagttt
     8641 gttgcggcgt tgttgtacca cgggaaccag tattcactcg aaagctgctc atcagggaga
     8701 ccaatcatct cagaatagct gaatcgtgaa ccgggttcct tccagattac gcgaagagca
     8761 gcaaggatat taacaatatt cgtgctgttg atccttgcgg gcccattgtt cacgccggca
     8821 tacccttctc tcatgcttct tcccggaggt agggcaaaag ttgtcaaatt gttattagcg
     8881 atcgttacgg ccacctctgc acagcctgaa gaggggttga agcctggagt gtaattttga
     8941 ttaggcatat ccgaagttac gttggtatag gtgcggctcg gaagagggaa ggta
//
LOCUS       DASTJP010000241         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1102130, whole genome shotgun sequence.
ACCESSION   DASTJP010000241 DASTJP010000000
VERSION     DASTJP010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102130"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VFQ23_07295"
     CDS             complement(<1..1533)
                     /locus_tag="VFQ23_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9906427.1"
                     /translation="MKKILPAWRGLTVQLLAITVLPLTLLLLLIAFGSVSFHQQDMRA
                     LVGERDERAVQAAAAALASELHHRAANISNLVALAELAADVSSISATEDLTSDFDGGL
                     AYLDMDKRLITNTYPSQIWDLVAQNDISLASSTGIEPVFSSAFLDPVSNRFFVIVSQV
                     IPTRNIIVAGAFSPQTLAEETLFTSYPDSTHVTIFLLDPSHQILFSSGPLAMEKISPN
                     HPGITEALRGESGTTYVRQDGIEHVVAYSPITPTGWALVTEEEWEMVTSPSLQLTQIA
                     PLVIVPAFILALIALWFVASRIVQPLQRLESKAAALAWGDFETIKEPVGGISEVQHLQ
                     MELTEMSRKVQAAQEGLRDYIGAITSAQEEERNRLARELHDDTIQAVIALRQRVQLAQ
                     KSVKDHTGRQSLRELESLAEQTIENLRRLTRALRPIYLEDLGLVTALEMLAREMSQKN
                     GLIVGFQKTGQEHRLSREVELSLYRIAQEALNNVIKHSKATHADLKIAFAASEIKLEV
                     LDN"
     gene            complement(1530..>2192)
                     /locus_tag="VFQ23_07300"
     CDS             complement(1530..>2192)
                     /locus_tag="VFQ23_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HET9906428.1"
                     /translation="SDIDFVTVLNRRLTPRDTGKLQSIHQELEKNFPQWKMLGSYIQP
                     GDLGKLDGNIQPHPHYHDGVLHQDGHNELNAVTWWELKHHGIAVVGIEPQNLSFTLDW
                     NVLITEMMENLNTYWAGWTSRPVRIVMLYSDWGIQWAVTGILRQFYTFRENTITTKIR
                     AAEYALGCLPKRWHPLIQEAINIRQKRSRSTYWFRIARTFEAVNFLRFIIQTCNAKYP
                     QK"
BASE COUNT          556 a          538 c          545 g          553 t
ORIGIN      
        1 attatccagg acttccagtt tgatctctga agcggcaaag gcaattttca aatcagcatg
       61 agtggctttg gagtgtttga tcacattgtt aagagcttcc tgcgcaatac gatagagtga
      121 taactccact tcacgcgaaa gacggtgttc ctgacctgtt ttttggaaac ccacaataag
      181 accatttttt tgactcatct cgcgtgccag catttccaat gcggtgacaa gccccagatc
      241 ttcaagataa attggtcgta gtgcacgcgt gagacggcgg aggttctcga tcgtctgctc
      301 ggcgagggat tccaattccc tgagtgattg tctgccagta tggtctttaa ccgatttctg
      361 tgccagctgc acgcgctgtc tgagcgcaat gactgcctgg atcgtgtcat catgtagttc
      421 gcgtgcgagg cggtttctct cttcctcctg tgccgaagtg atggcgccga tgtaatcgcg
      481 caatccctcc tgtgcagcct gtactttgcg tgacatctcg gtcagttcca tttgcaggtg
      541 ttgcacttcg gagatgccac cgacaggttc cttgatggtc tcaaaatcgc cccatgccag
      601 cgcggcggct ttcgactcaa gtctttgaag cggctgaacg atgcgcgatg ccacaaacca
      661 taatgcaatc aatgcaagga taaaagcagg gacaatcacc agcggcgcaa tctgcgtaag
      721 ttgcagagat gggcttgtga ccatctccca ttcctcttcc gtaaccagtg cccaacccgt
      781 aggtgtgatg gggctataag ctactacatg ctcaatgccg tcctgcctga cgtacgttgt
      841 gccactttct ccacgcaggg cttcagtgat cccaggatga ttgggggaga ttttctccat
      901 tgcaagaggt ccgcttgaga acaaaatctg atgcgatgga tcaagaagaa aaatcgtgac
      961 gtgcgtactg tcaggataag aagtgaagag cgtctcttct gcaagagttt gaggcgaaaa
     1021 agcccccgcc acaatgatgt ttcgcgtggg aatcacctgc gacacaatca caaagaatct
     1081 atttgagaca ggatcaagga aggcagagga gaaaacgggc tcaatgccgg ttgaggaggc
     1141 gagggaaata tcattttgag cgaccaggtc ccaaatttga gagggatagg tatttgtgat
     1201 aagccgttta tccatatcca gataggccag cccgccgtcg aagtcagagg taagatcttc
     1261 agttgcagaa atggacgata cgtctgcagc caattctgcc agcgcgacaa gatttgagat
     1321 attggcagcg cgatggtgaa gctctgaagc tagagccgcc gcggcagctt gcacagcccg
     1381 ttcatcgcgt tcaccgacga gtgcgcgcat atcctgctga tgaaacgaga cactcccgaa
     1441 cgcgatcaac aatagaagca acgtaagtgg caaaacggtg atcgcaagca actgtacagt
     1501 gagcccacgc caggcaggca gaatcttttt catttttgag ggtacttggc gttacaagtt
     1561 tggatgataa atcttagaaa attaactgct tcgaatgtcc ttgcaattct aaaccagtat
     1621 gtagatctac ttcgcttctg ccgaatatta atggcttcct gaattaaggg atgccaacgc
     1681 ttcggcaaac agccgagagc atattcagca gctctaattt tggtagtaat tgtattttct
     1741 ctgaatgtat aaaactgacg taatatcccg gtgaccgccc attggattcc ccaatcagag
     1801 tacagcatga caatacgaac gggccggctt gtccagcctg cccaatatgt attcaagttt
     1861 tccatcatct ctgtgatgag tacgttccag tctaaagtga aggataggtt ctgaggctca
     1921 atgccaacca ctgcaatgcc gtgatgcttt agctcccacc atgtcacagc gttcagttca
     1981 ttatgcccat cctggtggag gacgccatca tgataatgcg gatgaggctg tatgttccca
     2041 tcgagcttgc ccagatcacc cggctgaatg taactcccca acattttcca ttgaggaaag
     2101 ttcttctcca attcctggtg aatgctctgc aattttccgg tatcccgggg agtcagacgt
     2161 cggttcaaga ccgtgacaaa atcaatatca ct
//
LOCUS       DASTJP010000242         3873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_880593, whole genome shotgun sequence.
ACCESSION   DASTJP010000242 DASTJP010000000
VERSION     DASTJP010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880593"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFQ23_07305"
     CDS             <1..301
                     /locus_tag="VFQ23_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HET9906429.1"
                     /translation="TDPDNTDAKTLLRAAEIGREAGLHFVYAGNLPGQVGEYEDTFCV
                     QCNHRLIRRVGYIIKEYHITSEGTCPKCHTSVPGLWHKDPSKVVLNGIGIPLPVY"
     gene            427..2151
                     /locus_tag="VFQ23_07310"
     CDS             427..2151
                     /locus_tag="VFQ23_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9906430.1"
                     /translation="MKRKLFALLSLLVLASMILTACGGAAATEAPAAEPTEPPATEMP
                     ATEAPATEAPMSDFVGDKVEAESCDYGGEFQSIEAVDQYTVKFSLCYPDGAFLNKVAF
                     AAFQILDKDYLNEMGGDTAKISEAPNGTGPYTLGEWVRGDHITFEANPNYWGEAPTIQ
                     TLIFRWSTEAAQRLLELQSGTVDAVFAPAAEDFEAIEADPNLVLIPYDSPNVMYLGMN
                     NTIPPFDNPQVRQAISMAINKQQIVDLFYPPGSTIAEQFVPPSISPGFSTTGDGASWY
                     EYDPEGAKALLAEAGFPDGFETTFSFRDVTRIYVARQPQVAQEIQAQLAEIGVTINIT
                     PMESGTFLESVAAGEQPFFLLGWGLDYPDATNFYDFHFAGGVPRFGDQWPDIVEQIRA
                     AAQAPEASVRQEHYDQVAALIKEHTPMIPVAHGAAADVFSASVGNVKIGALNENFQEM
                     TTDDGQLVWIQGGEPISLWCGDETDGETFRACLQIYDSLLAYEFGGTQAVPALAESWE
                     SNEDGTEWTFTLRQGVTFHNGATFDANDVVATYSALWDASNPNHVGNSGAFEYTVGFF
                     GALLNAPQ"
     gene            2254..3330
                     /locus_tag="VFQ23_07315"
     CDS             2254..3330
                     /locus_tag="VFQ23_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906431.1"
                     /translation="MYQFLFRRLILLIPVIFGITFVTFALARILPGDPCYAALGERAT
                     EEQCNEYREREGLNDPILVQFGRYLNDIAHGNFGESLKDRRPVMQIVLERLPMTLEVT
                     LGAMLFSTFFGVLLGVISAVRRNTAVDVGTMIGANIGVSMPVFWLGLMLAYFFAIILK
                     ETPFWIPPSGRLSSGLSLPSLAETFNLQNVDGLWGAIVTFASNMVTFNALITGNFEVL
                     RDALWHLILPCVAVGTIPLSIIARMTRSSLLEVFGLDYVRTARAKGLRERVVISKHAL
                     RNALLPIVTVIGLSFGGLMSGAVLTETVFSLPGVGTQLVRAILSRDYAVVQAFTVVIA
                     LIFVFVNLIVDLSYVFLDPRIRMQ"
     gene            3338..>3873
                     /locus_tag="VFQ23_07320"
     CDS             3338..>3873
                     /locus_tag="VFQ23_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906432.1"
                     /translation="MTSTAAPLQLEGLKTPVSRTPLQNALRRFIRHRSARVGMTILGL
                     LVFVAIFASVLAPHDPIKPLRDAKRRSPPCIHFLGCPADQPQHFFGIDSNQRDLLSRI
                     IYGSRLSLEIGLATVTFAIIIGMALGALGGFAGGWTDNIIMRFMDVLLAFPSLLLSIA
                     IVAVLGPGLINALIAIAFV"
BASE COUNT          865 a          991 c          984 g         1033 t
ORIGIN      
        1 gaccgaccca gataataccg atgccaaaac ccttttgcgc gcagcagaga tcgggcgtga
       61 agcaggtctc cattttgtgt atgcgggcaa tttgccgggg caagtgggag agtatgagga
      121 cacattctgt gtgcaatgca accatcggtt gattcgaaga gtaggataca ttatcaagga
      181 atatcacatc acaagtgaag gaacctgtcc caaatgccat acaagcgtac cgggcctatg
      241 gcacaaagac ccctctaaag tagtgttaaa tggtatcggg atcccattgc cggtctacta
      301 aagaacgatt tgctgtataa tcattttaat attcggaagg aggtgcttcg cacaaagtac
      361 aattccgaaa tccctatctg tagacgtaat tgaatcccaa tccaatttct ctctaggagg
      421 agaaacgtga aacgcaaact atttgcttta ctgtcattgc tcgtgctcgc cagcatgatc
      481 ctgacagcct gtggcggtgc ggcagcaacc gaagcgcctg cggcggaacc tacggaacca
      541 cccgcaacag aaatgccagc aacggaagca ccggcaactg aagcgccaat gtctgatttt
      601 gtgggtgata aagtggaagc cgaaagttgt gactacggtg gtgagttcca atccattgag
      661 gctgtagacc aatacaccgt caagttctcc ctgtgttacc ctgacggtgc cttcctgaac
      721 aaagtggcct ttgccgcgtt ccagatcctg gacaaggact acttgaatga aatgggtggt
      781 gacactgcca agatcagtga agctccaaat ggcaccggcc catacacatt aggcgaatgg
      841 gttcgcggtg accacatcac cttcgaagcc aatcccaatt actggggcga agcaccgaca
      901 atccaaaccc tgatcttccg ctggtcgaca gaagccgctc agcgtttgct cgagttgcag
      961 tcgggtacgg ttgatgccgt ttttgcacct gctgctgagg acttcgaagc gatcgaggct
     1021 gaccccaacc tggtactaat cccttacgat agccccaacg ttatgtacct tggtatgaac
     1081 aataccatcc caccttttga taaccctcag gtgcgccagg ccatctctat ggcgattaat
     1141 aagcagcaaa tcgttgacct tttctacccg cctggatcca cgattgcaga acaattcgta
     1201 ccccccagca tctcacccgg atttagcaca actggtgatg gcgcttcatg gtatgagtat
     1261 gacccagaag gtgccaaggc actcctggca gaggctggtt tcccggatgg ttttgaaaca
     1321 accttttcct tccgtgatgt cacacgcatt tacgtggcac gccaacctca ggtagctcag
     1381 gaaattcagg cacaactggc tgaaatcggc gttacgatca atatcacgcc aatggaatcc
     1441 ggcaccttcc ttgaatcagt agccgctggc gaacagccat tcttcctgct cggctggggc
     1501 ttggactacc cggatgcgac caacttctac gacttccact ttgccggcgg cgttccccgc
     1561 tttggcgacc aatggcctga tatcgtggag caaattcgag cagctgctca agctcctgaa
     1621 gcgtctgtgc gccaggaaca ctatgatcag gtagcggcat tgatcaaaga acatacgccg
     1681 atgatccctg ttgcccacgg tgcggcggcg gatgtcttca gtgcaagtgt cggtaatgtg
     1741 aagatcggtg ctctcaacga aaacttccag gagatgacca cagatgatgg tcaactggtc
     1801 tggatccagg gcggtgagcc gatcagtctg tggtgtggtg atgagaccga cggtgagacc
     1861 ttccgcgcct gcctgcagat ttatgactcg ctgcttgcct acgaatttgg cggcacgcag
     1921 gctgtccctg ctttggctga gtcctgggaa tccaatgagg atggtaccga gtggacattc
     1981 accttgcgac agggtgtgac attccacaac ggtgccacct tcgatgcaaa cgatgtggtt
     2041 gcaacgtatt ccgcactttg ggatgcgtcc aatccgaacc acgttggtaa cagtggcgct
     2101 tttgagtata cggttgggtt ctttggtgcc ctactcaatg ccccacaata atcgattgat
     2161 ctatcccgtt tgctgagtag ttcgtgaagt acaatgtgcc ctgaagattc ttaagagttt
     2221 tcagggcaca tattatcatt cagagctttc tttatgtatc agtttttatt tcgtcgctta
     2281 atcctgctaa tcccggtgat ttttggaatc acgttcgtaa cgtttgcact ggcgcgcatc
     2341 ctgccgggcg atccatgtta tgcggcgctc ggtgaaagag cgaccgagga gcaatgcaac
     2401 gagtatcggg aaagggaagg cttgaatgac ccaatcctgg ttcaattcgg acgctatcta
     2461 aacgatatcg ctcatggcaa cttcggtgag tcgttaaaag accgacgtcc agtgatgcag
     2521 atcgtgctgg agcgcttgcc catgactctg gaagtaacac tgggggccat gctcttctcc
     2581 acattcttcg gagttttgct gggtgtaata tctgctgtac ggcgtaatac ggccgtggat
     2641 gtaggcacca tgatcggtgc caatattggt gtctctatgc cagtattttg gctgggtttg
     2701 atgcttgcct attttttcgc gatcattctt aaagaaacac ctttttggat tccaccctcg
     2761 ggtcgccttt cttcaggact ttctctccct tctttggctg aaacattcaa cttgcagaac
     2821 gtcgatgggc tttggggagc gatcgttacg tttgcctcca atatggtcac attcaacgcg
     2881 ctcattaccg gtaattttga agtcctgcgt gacgcgttat ggcacttgat tttgccctgc
     2941 gtcgcggttg gcacaatccc cttatcgatt atcgcgcgta tgacccgctc gagcctgctt
     3001 gaagtcttcg ggctggatta cgttcgtacg gcgcgtgcca aaggtttgcg tgagcgggtc
     3061 gtcatcagca aacatgcttt gcgcaacgct ctacttccta ttgtcactgt gattggtcta
     3121 tcgtttggtg ggttgatgag cggcgcagtg ttaactgaga ctgtgttttc attgcccggt
     3181 gttggtacac aacttgtgcg agcgattctt tcaagagact atgcggtcgt tcaagcattc
     3241 acagtagtaa ttgctcttat ttttgttttc gtaaatctta tcgtcgattt atcctatgta
     3301 tttctagatc ctcggatccg catgcaataa tcagattatg acatcaactg ccgctccgct
     3361 ccaactggag ggactcaaaa ctcctgtctc gcgcacacct ctgcaaaatg cgctgcgacg
     3421 tttcattcgc catcgttcgg cgcgcgttgg aatgaccatt ctagggctgc tcgtctttgt
     3481 agcaatcttt gcatccgtat tggcaccaca tgacccgata aaaccactcc gcgatgcaaa
     3541 gcgccgctcg ccgccctgta ttcatttcct ggggtgcccg gccgaccagc cacaacactt
     3601 ctttggcatt gatagcaatc agcgtgattt gctttcccgc atcatctatg gttcgcgtct
     3661 ctctttggag atcgggcttg cgacggtgac gtttgccatc attatcggca tggcattggg
     3721 tgcattgggc ggctttgcag gcgggtggac cgataacatt attatgcggt tcatggatgt
     3781 cttacttgcg tttccaagtt tgttattatc cattgcaatt gtggcggtgt tgggtcccgg
     3841 cttgatcaat gctttaatcg cgattgcatt tgt
//
LOCUS       DASTJP010000243         9845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_852493, whole genome shotgun sequence.
ACCESSION   DASTJP010000243 DASTJP010000000
VERSION     DASTJP010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852493"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFQ23_07325"
     CDS             complement(<1..436)
                     /locus_tag="VFQ23_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9906433.1"
                     /translation="MSTSINTFQRTLGRSGLQVSPMGLGCWAIGGVWSWLDGPGGWGD
                     IDDNESVRAIQAALDLGINFFDTAANYGTGHSERILARALEGKRDKAVIATKFGFNVN
                     EAEKRVRFREDDHLLHVREECEASLRRLNTDIIDLYQLHVWNY"
     gene            complement(447..842)
                     /locus_tag="VFQ23_07330"
     CDS             complement(447..842)
                     /locus_tag="VFQ23_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9906434.1"
                     /translation="MDIQKDKLTIQEVSQATGLTTHTLRYYERIGLIHPIHREENTRR
                     CYTADDVGWIDFLMKLRATGMSIKDMQRYAELQRQGDVTLPERVEMLKSLRDKVESHM
                     EEMNEHLKLIYYKIDYYQNVVEEQMLETS"
     gene            complement(991..1584)
                     /locus_tag="VFQ23_07335"
     CDS             complement(991..1584)
                     /locus_tag="VFQ23_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9906435.1"
                     /translation="MKPTIFNTPILSTLYHYIAKTLMRLTGWRVDGKLPDIPKFVLIG
                     APHTSSWDFVLFLGVIFSLRANVRFMGKAELFRPPWGRFFYYCGGVPVDRKKSTGLVE
                     QMVKVCNESEQFILTIAPEGTRHHVSDWKQGFYHIAKGAGIPIVLAIVDGKHKTVRIG
                     QVFHPTENAEADLKAIKEFFEGIVGINPRRKYITLQS"
     gene            1654..2502
                     /locus_tag="VFQ23_07340"
     CDS             1654..2502
                     /locus_tag="VFQ23_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9906436.1"
                     /translation="MNLKSMVRSGFYRAEKLLQRIRFVSAPADLPTLLGISFPKSGTH
                     LLDQILLGFSSVAPYPKRLHSFYAEYEGESGRKRTPEQAVEWIKSLHSRDVASAHLFA
                     RNEIIELVCSSSFIPFFIFRDPRDVAVSHVFYVTEMETSHVHHDYYKSLKDFDARLNV
                     SILGRPDSDIEFPNIGERFAPYQGWLDQPAVLKIHFEDFVNDRTSTLNNIIDHFLARV
                     PLQTPRPLILDSLESSINPSKSPTFRSGKTGEWKKSFTEEHKKIFKDVAGDLLVRLGY
                     ERNNDW"
     gene            2554..3015
                     /locus_tag="VFQ23_07345"
     CDS             2554..3015
                     /locus_tag="VFQ23_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9906437.1"
                     /translation="MSIKSLETLTVWTKAKEFALRIYREVLPLLPPEEKWNIDQQLRR
                     ASNSIPANIAEGYGRFYYQEIIRFCYISRGSLEETLSHLAMCYELKYISKTLFDSLEQ
                     DGEKLTQLINGYVGYLKRSKQGQNEPGANHTVREDIASYETDFSEDSKDIE"
     gene            3051..3983
                     /locus_tag="VFQ23_07350"
     CDS             3051..3983
                     /locus_tag="VFQ23_07350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9906438.1"
                     /translation="MNCSIVIRAYNEEKHIGRLLEGIRHQTLQDVEIILVDSGSTDKT
                     VSVAETFGAQIIRIPSDEFTFGRSLNFGIRAATHELIVIASAHVYPVYHDWLETLLRP
                     FEEQRVALTYGKQRGPESAKYSEQQIFQQWYPNHSKPKQETAFCNNANAAIRKNLWES
                     HPYDETLTGLEDLAWAKWTKEQGFDIAYVAEAEVIHIHNETPHGVFNRYRREAMAFKN
                     IHPESHFNVYDFVRLTTMNILSDLWHSAQDRLLWKNIASIFWFRFMQFHGTRTGYRET
                     GLVTPQLRETFYYARERKMKENKSRDIDPIRYNQ"
     gene            3987..4667
                     /locus_tag="VFQ23_07355"
     CDS             3987..4667
                     /locus_tag="VFQ23_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906439.1"
                     /translation="MSTSLSQDFINNVRVLYQLGLDEICSGKVVRYGINGYTIHAAPA
                     YVSAIASLEAFVNETLLGIFSKSVYQDSALWMLDSDWIEKLELKQKLIIVPQLLFSQT
                     FKRNELPFQDMQLLFRIRNDVVHYRMKFPKDKNDLPKYVNELEKRKIALSSPEPDKGD
                     FIWVHKICTTEGIRWANNTICKTVHKLVDFVSDMQERNIIFSLASNFREIPESFPKNF
                     LKQMGIED"
     gene            4676..5269
                     /locus_tag="VFQ23_07360"
     CDS             4676..5269
                     /locus_tag="VFQ23_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9906440.1"
                     /translation="MLNPPLDSFHPKSRAEWRKWLEENHTRTEGVWLVMYKKGTGKPR
                     VDYAEAVEEALCFGWIDSKGNKLDDERSMLWMAPRKSKTGWSKLNKERIKKLIKAKLM
                     TPPGLAKINAAKKDGTWNALDAIEALEIPPDLAKAFAENKIAKEYFDAFPRSAKRAIL
                     EWIASAKRTETRANRIEETVTKAEQNIRANQWRQGLE"
     gene            5333..6007
                     /locus_tag="VFQ23_07365"
     CDS             5333..6007
                     /locus_tag="VFQ23_07365"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HET9906441.1"
                     /translation="MLKIAALVPMRHHSQRVPGKNYRPLAGKPLFHHVLHSLLAVPEI
                     DEIVVDTDSEPIMDGVRAEFPQVKLINRPENLRADDVPMNDILIHDTDQVQAEFYLQT
                     HSTNPLLKPETISHAIQSLVTNYPKYDSLFSVTRLQTRLYDQQGEALNHNPRELIQTQ
                     DLPPVYEENSCIYIFTRENLIAKRHRISDHALMFEIDADEAWDIDEELDFEICDFLLK
                     RQVRNA"
     gene            6017..7525
                     /locus_tag="VFQ23_07370"
     CDS             6017..7525
                     /locus_tag="VFQ23_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9906442.1"
                     /translation="MTKTLTRWFSIPWYPLLFGAYPVLALLAENIGEVKVEASWRSIL
                     VCVALSVLLFGLLKLALRDWHRAAFLSTLWMILFFSYGHIHILLTEKLEDFDFTPWLL
                     LTWLLLAVGAAVWAIRKPPSSAGALNVIALGLVATSIVQIVPGIEKTGAHTLGAKNAP
                     VQELSLPQTPPDVYYFILDSYGREDLLEQAYGYDNSEFVSALRGRGFYVAECSQSNYV
                     RTEISVSSSLNMSYLQDLDPAFEPESTRRRVLWNSFKHSATRYNFESLGYKTIAFATG
                     FAWSEFDDADVFYTPPPFLAGMTEFETLFLQTTLALHAKDLGWIDADAIMGQNFRDRT
                     LLVFDRMDDIARMPEPTFAYVHVISPHPPFVFGPNGESTNPADFWNEDQEYPGNLYAK
                     GYQNQLTYLNKKVLEAVDTLLSESDTPPIIVFQGDHGPWLQPKNKRMWILNAYYLPNH
                     NDKLYPTISPVNTFRLIFDQYFGGTYDLLDDVSYFSPVPKLYEFSEVPNNCK"
     gene            7522..8502
                     /locus_tag="VFQ23_07375"
     CDS             7522..8502
                     /locus_tag="VFQ23_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET9906443.1"
                     /translation="MKYTVLLTAPYMIPFVDRFKPVFDQYDIEVIVPDVKERMEEADL
                     LDYAGQFDGAICGDDRYTERVISACAPRLKVISKWGTGIDSIDAAACSRYEIKIGRTL
                     NAFTLPVADSVFGYILAFARRQPWMDREMKSGKWEKIPGKSLSESVLGVIGLGNIGKA
                     VTRRAMAFGMKVYGTDIIEIDPKFIAETGIEMTDLASLLSNSDYISINCDLNPTSHHL
                     MDSDTFALMKPNAILINTARGPIVDEKALITALQNAQIAGAALDVFEHEPLPLDSPLL
                     KMDNVMLAPHNSNSSPGAWEKVHWNTIRNLLDGLGIPYSYTDFTGHTENA"
     gene            8520..8945
                     /locus_tag="VFQ23_07380"
     CDS             8520..8945
                     /locus_tag="VFQ23_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9906444.1"
                     /translation="MEPKKKFEGRYSELTGRILGAFFELHKELGFGFSEKIYETALEV
                     LLTELGLIVEKQKDIFVYFHGRIIGEYKADMVINGVVLLEIKSVERLIDAHDAQLLNY
                     LKATEVEVGLLLNFGRQAEFRRKIYDNPRKGSLSWAKIP"
     gene            9003..9758
                     /locus_tag="VFQ23_07385"
     CDS             9003..9758
                     /locus_tag="VFQ23_07385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9906445.1"
                     /translation="MTKTILITGAAGGIGRATLSLFHEKGWRVIGVDRSEFGDNFPQD
                     GLFIQSDISRAEDMQSIFDQAHAFSESLEALVNNAALQVAKPLVETTVEEWDAVMASN
                     LRSVFLGVKLAHPLLKAGSGGAVVNVSSVHAIQTSANIAAYAASKGGLLALTRAMAIE
                     FAPDNIRVNAILPGAVDTPMLRAGLGRGHVGHGDVQERLDNLARKTVSGKVGKPEEIA
                     HAIYFLADNEQSSFMTGQALVIDGGATARLSTE"
BASE COUNT         2611 a         2236 c         2337 g         2661 t
ORIGIN      
        1 ggtaattcca aacatgcaat tgatacaggt caatgatatc cgtgttcaaa cggcgaaggc
       61 tggcttcgca ttcttcgcga acatgcaaca agtggtcatc ttctcgaaaa cgtacgcgct
      121 tttcagcttc gttgacattg aacccaaatt ttgtcgcgat gacggcttta tcgcgtttgc
      181 cttcgagggc acgtgccaaa atccgttcgc tgtgacctgt cccataattc gcggctgtat
      241 caaagaagtt gatgcccaga tccagggcag cctggatggc gcgcacggat tcattgtcgt
      301 caatgtcacc ccagccgcct ggtccatcca gccaagacca tactcctccg atcgcccaac
      361 aacctaaccc cattgggctg acttgcagtc ccgatcgtcc caacgtccgt tgaaatgtgt
      421 tgattgatgt actcattttt ttcctttcag gatgtttcca acatttgctc ttccacaacg
      481 ttttgataat agtcaatctt gtaatagatc aattttaagt gttcgttcat ttcttccatg
      541 tgcgactcca ctttatcacg cagcgacttc aacatttcca cacgttctgg taaggtcacg
      601 tcgccctgtc tctggagctc tgcgtatcgc tgcatatcct tgatcgacat gcccgttgca
      661 cgcaatttca tcaggaaatc gatccagccc acgtcgtctg cggtataaca ccgtcgggta
      721 ttttcctcgc gatggatcgg gtgaattagc ccgatacgct cgtaatagcg cagtgtatgc
      781 gtggtcagac ctgttgcctg agagacttct tgaatggtga gtttgtcttt ttggatatcc
      841 atgcccctag tttacatctt agagtatgct ctaagtcaag ccccaatttt gtcaattcga
      901 ggagaagtgc ccgggcgtag cgacgaagca atcctatcgc tgggaaagaa atgctttact
      961 gtgcatatca ccggtattcc ctctgatagc ttaactttga agtgttatat atttccttct
     1021 cggattgatt cctacgattc cctcgaaaaa ttccttgatc gctttcagat ctgcttccgc
     1081 attttcagtc ggatgaaaca cctgcccaat acggaccgtc ttatgcttgc cgtctacgat
     1141 tgccagcacg atcggaatcc ctgcaccttt agcaatatga taaaagccct gcttccaatc
     1201 actcacatgg tgacgtgtgc cttcgggagc aatcgttagg atgaactgct cagattcatt
     1261 gcagaccttc accatctgtt ccaccagtcc tgttgacttt ttgcggtcca caggcacacc
     1321 gccgcaataa tagaagaacc gcccccatgg tggacgaaat aactctgcct tacccatgaa
     1381 acggacattt gctcttaggc taaaaatcac gccaagaaat agtacaaaat cccaactgga
     1441 ggtatgtggc gcgccgatca atacaaattt tggaatatcc gggagtttcc cgtccacgcg
     1501 ccaacccgtt agccgcatca gtgttttggc gatgtagtga tacagggtac tcaggatagg
     1561 ggtattaaag attgtaggct tcatcgtcta tgtcacttat aaccctgaac tatcgtttct
     1621 gtatcggaag caattttgtg gttaaatacg cgcatgaact tgaagtcgat ggtgcgttcc
     1681 gggttctatc gggcagagaa acttttacaa cgaatccgtt ttgtatctgc gcccgccgac
     1741 ttgccaactt tgctgggtat ctcttttcca aaaagcggta cgcatttgct tgatcaaatc
     1801 ctgctcggtt tttcaagtgt ggcgccgtac ccaaagcgcc tgcattcgtt ttatgcggaa
     1861 tacgaagggg aaagcggacg caaacgtact cctgaacaag ctgtggagtg gataaaatct
     1921 ctccattcaa gggatgttgc gtcagcccat ttgtttgcgc gtaatgaaat catcgagctg
     1981 gtttgttctt cttcattcat tccatttttc attttccgtg acccgcgcga tgtagcagtc
     2041 tcacacgttt tttacgtgac cgagatggag acgagtcatg ttcaccatga ttactataaa
     2101 tcacttaagg attttgacgc gcgcctcaac gtaagtattt tgggaagacc cgattcagat
     2161 attgaatttc cgaatattgg tgagcgattc gctccatacc agggatggct ggatcaaccg
     2221 gctgtcttga agatccattt tgaggatttc gtgaatgacc gcacctcgac gctgaacaat
     2281 atcattgacc actttctggc ccgtgttccg cttcagactc cccgaccatt gattctcgat
     2341 tctttggaat catccatcaa cccgagcaaa tcacccacgt tccgttcagg gaaaactggt
     2401 gaatggaaga aatcttttac agaggaacat aagaaaatct tcaaagatgt cgctggagat
     2461 ttgttggtac gattgggata tgaaaggaat aatgattggt aagggtggga gtcgggaatt
     2521 ggtattcgta ttggcaatta tggagaatat gcgatgagca taaaaagttt ggaaacactt
     2581 acggtttgga ccaaagcaaa agaatttgct ttacgaatat atcgtgaagt tttgcctcta
     2641 ttgccgccgg aagaaaagtg gaacatcgat cagcaattac gacgagcttc aaatagcatt
     2701 cctgctaata ttgctgaggg atatggacgt ttctattatc aagaaattat cagattttgc
     2761 tatatttctc gtgggtcact tgaggaaaca cttagccatc ttgccatgtg ctatgagcta
     2821 aaatatattt ctaaaactct ttttgattct ttagaacaag atggggaaaa attgacgcag
     2881 ttaattaatg gttatgttgg ttacttaaag cgtagtaaac aggggcaaaa tgaaccgggt
     2941 gccaatcaca ctgtccgcga agatatcgca tcctatgaaa ctgatttctc cgaagactcc
     3001 aaagacattg agtagcgatt tccgattccc catttccgac tccctaccct atgaactgct
     3061 ccatcgttat ccgtgcttat aacgaagaaa aacatatcgg gcggttgctc gaagggattc
     3121 gtcaccaaac gctccaggat gtggagatta tcctcgtcga ttctggctcg acggataaaa
     3181 ctgtttccgt tgctgaaacc tttggcgctc aaattatccg aatcccttcc gatgaattta
     3241 cctttgggcg ctccctcaat tttggcattc gagctgccac gcatgaattg attgtcattg
     3301 cgagtgcgca tgtttatccg gtgtatcatg actggctgga aactctgctt cgtccctttg
     3361 aagaacaacg ggtcgctctc acctatggga aacaacgcgg acctgaatct gcaaaatatt
     3421 ccgagcaaca gatttttcag caatggtatc ccaatcatag taaaccaaaa caagagactg
     3481 cgttttgcaa caacgccaat gcggcaattc gaaaaaattt gtgggagagc catccatacg
     3541 atgaaacact gacggggttg gaagatcttg cctgggcgaa gtggacaaaa gagcagggat
     3601 tcgatattgc gtatgtggcg gaagcagaag taatccacat ccataacgag acaccgcacg
     3661 gtgtgttcaa ccgctaccgc cgcgaagcca tggcatttaa aaacatccat cctgaatcgc
     3721 acttcaacgt atatgatttt gtgcgtctga cgacgatgaa tatactgagc gatctgtggc
     3781 actcggcgca ggatcgtctg ctttggaaaa acatcgcatc gattttctgg ttccgtttta
     3841 tgcagtttca tggaacaaga acgggatacc gcgagaccgg tcttgtgacg ccgcaacttc
     3901 gtgagacgtt ttactacgcg cgtgaaagaa agatgaaaga gaataagagc agagatattg
     3961 acccgattcg atataatcaa taaatcatga gtacttctct atctcaggac ttcataaata
     4021 atgtacgggt tctttatcaa ttagggttag atgaaatttg ttcaggtaaa gttgtcaggt
     4081 atggaattaa tggatacact attcacgctg ctcctgcata cgttagcgca atagcttcgc
     4141 tagaagcatt tgtgaatgaa acgcttttgg gaattttttc taaaagtgta tatcaggatt
     4201 cggcgttgtg gatgcttgac tctgattgga tcgaaaaact agaactaaaa caaaaattga
     4261 taatagtccc gcaacttctc tttagccaaa catttaagag aaacgagctg ccgtttcagg
     4321 atatgcagct attatttcgc attcgcaatg atgttgttca ctacagaatg aaatttccca
     4381 aagataaaaa tgacttgcct aaatatgtga atgaattaga gaaacgaaaa atagctttat
     4441 cgagcccaga gccagataaa ggcgacttca tatgggtaca taagatatgt actactgaag
     4501 gaataagatg ggctaataat acaatctgta aaacagtaca taaattagtt gactttgtat
     4561 cagatatgca ggaacgcaat attatttttt ccttagcatc caattttaga gagattccgg
     4621 agtcgtttcc taaaaatttt ctaaagcaaa tggggattga agactaatgg gaattatgct
     4681 aaatcctcct ctagactcct ttcaccccaa atcccgcgcg gagtggcgca agtggctcga
     4741 agaaaatcac actcgaaccg aaggcgtgtg gctggttatg tacaaaaagg gcacaggtaa
     4801 gccacgtgtt gactacgccg aagcagtgga ggaagccttg tgtttcggct ggattgacag
     4861 taagggcaac aaacttgatg atgaacgatc catgttgtgg atggcgccgc gcaagtcgaa
     4921 aacaggctgg tcaaaactca acaaggaacg aatcaagaaa ctgatcaagg cgaaactcat
     4981 gacacccccc gggctggcga aaattaacgc cgcgaaaaag gatggcacct ggaacgcgct
     5041 cgacgccatt gaggcgcttg aaattccgcc agatcttgca aaggccttcg ctgagaacaa
     5101 aattgcaaag gaatatttcg atgcctttcc gcgttcggcg aagcgcgcaa ttctggaatg
     5161 gatcgcaagt gcaaaaagga ccgaaacccg cgcaaatcgc attgaagaga ccgtgacgaa
     5221 ggccgaacag aatatccgtg ccaatcagtg gagacaagga cttgagtgac tgtcatcttg
     5281 aagtagacag tcactttttg caaatgtggc aattcggtat aatcaatgac ctatgctaaa
     5341 aatcgccgcg ttagttccaa tgcgccatca ctcccagcgc gtgccaggta aaaattatcg
     5401 tccgttggcg ggcaagccac tcttccatca cgtgctgcat tctctgcttg ctgttcccga
     5461 gattgatgaa attgttgtag atactgattc cgaaccaatt atggatgggg ttcgggcaga
     5521 gtttccacag gttaagctca tcaatcgtcc ggaaaattta cgcgcagacg atgttccgat
     5581 gaacgatatt ttgatccatg atacagatca ggtgcaggca gagttttatt tgcagaccca
     5641 cagcacgaat ccgttgctca aacctgagac gatatcgcat gcgatccaat cactggttac
     5701 gaattaccca aaatatgatt ctctcttttc tgttacccgc ctgcaaacgc ggttatacga
     5761 ccagcagggg gaagcgctca atcacaatcc gcgcgaattg atccagactc aggatctacc
     5821 tccggtgtat gaggaaaatt cctgtattta catcttcact cgcgagaacc tgattgcgaa
     5881 gcggcatcgt attagtgacc acgcattgat gtttgagatc gatgccgacg aggcctggga
     5941 catcgacgaa gagctggatt ttgaaatttg tgatttcctc ttgaaaagac aagtaaggaa
     6001 tgcataactt tcgcgtatga caaaaactct aacaagatgg ttttcgattc cctggtaccc
     6061 gctcctattc ggcgcatatc ctgtactcgc cttacttgcg gagaacattg gggaggtcaa
     6121 agtcgaggca agctggcggt caatcctggt ttgtgtggcg ttgtctgtcc ttctattcgg
     6181 cttattgaaa ctggccctgc gtgactggca ccgggctgca ttcctgtcta ccttatggat
     6241 gatccttttc ttctcctatg ggcatatcca cattttgctg acagaaaaat tggaagactt
     6301 cgatttcacg ccctggttat tgctaacatg gcttctcctg gcggtgggcg cggctgtatg
     6361 ggcaattaga aagccacctt catcggctgg ggcattgaac gtgattgcat tgggacttgt
     6421 agctacatcg attgtgcaga ttgtgccagg aattgagaaa acaggtgctc acacacttgg
     6481 tgcgaaaaat gcaccggttc aggaattgtc tcttcctcaa actccgccgg acgtctatta
     6541 ctttatcctc gattcttatg gacgcgagga tttattggaa caggcctatg gatacgacaa
     6601 tagtgaattt gtaagtgcct tgcgcgggcg cggtttctat gtggcagaat gcagtcagag
     6661 caattatgtg cgtacggaaa tttctgtatc ctccagccta aatatgtctt atcttcagga
     6721 tcttgacccc gcctttgaac cagaaagcac ccggcggcgg gtgttatgga actccttcaa
     6781 gcacagtgcg acgcgttata actttgagag cctgggctat aaaacgattg catttgccac
     6841 aggttttgcc tggagtgaat tcgatgatgc ggatgtgttc tatacgccgc cgcctttttt
     6901 ggctgggatg acagaatttg aaacgctgtt tctgcagacg accctggctc tgcatgccaa
     6961 ggatttaggc tggattgatg ccgatgcaat catgggtcag aacttccggg accgaacttt
     7021 gctggtgttc gatcgaatgg atgatattgc gcgcatgcct gaaccgacgt ttgcttatgt
     7081 gcacgtgatc tcacctcatc cgccgtttgt atttggacca aacggtgaat ctaccaatcc
     7141 ggcagatttt tggaatgagg atcaggaata tccagggaat ctatacgcga aaggttatca
     7201 aaatcagtta acttatttga ataaaaaggt cctggaagct gtggatacgt tgctgtctga
     7261 atcagataca ccgccaatta ttgtttttca gggggatcac ggtccctggc tgcaaccgaa
     7321 gaataagagg atgtggattc taaacgcgta ctatttgcca aatcataacg acaagcttta
     7381 ccccaccatc tcgccagtca acacatttcg cctgatcttt gatcagtatt tcggcggcac
     7441 atatgatttg cttgacgatg tcagttattt ctcacctgta ccaaagttat atgagttctc
     7501 cgaagtgcca aataattgca aatgaaatac actgtccttc tcactgctcc ttacatgatc
     7561 ccatttgtag atcgcttcaa gcctgtgttc gatcagtatg atattgaagt gattgttcca
     7621 gatgtgaagg aacgcatgga agaggcagat ttgctcgatt atgctggtca atttgacggt
     7681 gccatttgtg gggacgaccg ctatacagag cgcgtgatct ccgcttgcgc gccgcgcctt
     7741 aaggtcattt caaagtgggg gaccggtatc gattcaatcg acgccgcagc ctgctcgcgt
     7801 tatgagataa agattgggcg taccttgaac gctttcaccc tcccagttgc agacagtgtt
     7861 ttcggctata ttctcgcctt cgctcgccgc cagccctgga tggatcgaga aatgaaaagc
     7921 ggaaagtggg aaaagatccc cggaaagagc ctgagcgaaa gtgtattggg agtgatcggt
     7981 ttgggtaata ttggcaaagc agtgacccgg cgcgctatgg cgtttggtat gaaagtttat
     8041 ggtaccgata ttattgagat tgatccgaaa ttcattgcag aaacaggcat agaaatgaca
     8101 gaccttgcat ccctgctttc taacagtgat tacatttcga tcaattgtga tttgaatccc
     8161 accagccacc acttgatgga ctccgatacg tttgcgttga tgaaaccgaa cgccatcctg
     8221 attaacacag cgcgtgggcc gatcgtcgat gagaaagcat tgattaccgc cttgcaaaat
     8281 gctcaaattg cgggtgcagc gctggatgta tttgaacacg aaccattacc gcttgatagc
     8341 cctctcctaa aaatggataa tgtaatgctc gcaccgcaca attcaaactc cagcccaggc
     8401 gcatgggaaa aagtccattg gaataccatc aggaatctat tagatgggct cggtatccca
     8461 tattcgtaca cggatttcac gggacacacg gaaaatgctt aaactgacgg aggttggata
     8521 tggagcccaa aaagaaattc gaaggaaggt attccgagtt aacaggaaga attcttggag
     8581 ctttctttga acttcataaa gaactgggct ttggcttttc tgagaagatt tacgagacgg
     8641 cactggaagt attgcttact gaacttggtt tgatcgtcga aaagcaaaag gatatttttg
     8701 tctattttca tgggagaata attggcgaat ataaggcaga tatggtcatc aatggtgttg
     8761 tgctgctgga aattaaatca gttgagaggc tgatcgacgc tcatgatgca cagttgctga
     8821 attatttgaa agcaacggaa gtcgaagttg gactcttatt gaatttcggt cgtcaagccg
     8881 agtttcgccg taaaatctat gataatccgc gcaaaggctc actttcctgg gcgaagattc
     8941 cgtaaaagtt aattcgtctc catcaaatcc gtgtgatccg tgtacgaaaa gaaattaagg
     9001 atatgacaaa aactattctc attacagggg cagcaggtgg tatcggtcgc gctaccttat
     9061 ctctctttca cgaaaaaggt tggcgcgtga tcggcgtgga tcgctccgag tttggtgata
     9121 attttccaca agatggcttg ttcatccaat cagacatttc acgcgctgaa gatatgcagt
     9181 cgatctttga tcaggctcat gcttttagcg agagcctcga agcactcgta aataatgccg
     9241 ccttgcaggt ggccaaaccc ctggtcgaaa cgaccgtcga agagtgggat gcggtcatgg
     9301 catccaatct gcgttcggta tttttaggcg ttaagctggc gcatcctctg ctcaaggcag
     9361 ggagtggtgg ggcagtagtg aatgtgtctt cagtccatgc gattcaaact tcagccaata
     9421 ttgccgccta tgcggcctcg aaaggtggct tgctcgccct gacgcgcgcc atggcaattg
     9481 agtttgcacc ggataatatt cgcgtcaacg cgatcttgcc gggtgcagtg gatacaccga
     9541 tgctccgtgc cgggcttgga cgcggtcatg tcggtcatgg cgatgtgcag gagcggcttg
     9601 ataacctggc gcgcaagaca gtcagtggca aagtagggaa acccgaagag atcgctcatg
     9661 ccatttactt tctggcggat aatgagcaat cctcgtttat gacaggtcag gccttggtta
     9721 tagatggcgg cgcgacggca cggctaagca ctgaataaac aatgaagcag actctcaaac
     9781 aaatcatccc gtccccactc tggaattttg cgcgttattc aaatgatgct cttcgccgtc
     9841 ttcca
//
LOCUS       DASTJP010000244         4105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_9072,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000244 DASTJP010000000
VERSION     DASTJP010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9072"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1073)
                     /locus_tag="VFQ23_07390"
     CDS             complement(312..1073)
                     /locus_tag="VFQ23_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9906446.1"
                     /translation="MTESIAQYPQVPIANSQLRHIKSSIVGREYQVKIRLPEDYANTT
                     KLYPVLYLLDGDHAFAMATDIVQYLIYGQHIPDLIIASPAYNSKKLPNEGGKNMRDHD
                     LIFWPTPYNDMTPGAAPYLEFFQQELIPFVESNYRVVPNDRTLWGYSSGGVFALFALF
                     EKPHLFQRYVIVDGFDDIFLKIEEAYAKQHEDLSARVCISAPPSDLDSKKLFDLLKAQ
                     KFPSFRAEFAQLNNLGHFAISAEGLTKGLVSVFGN"
     gene            complement(1313..1813)
                     /locus_tag="VFQ23_07395"
     CDS             complement(1313..1813)
                     /locus_tag="VFQ23_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906447.1"
                     /translation="MKENHFFRPGFQGFFISVVIILLAMSAMAVSTNYRYPAHPSCGG
                     ALGAGFPTLFICDAGLGGSPIDSWGKIDFADVFNRGIRPAGFMVNFLFYLALIWLVGL
                     MVSRISHNRIGARHVWWAAFISIGFVFGFLCAFLVFQSSSSYDPRVATSTPPGTFTPL
                     IATSIP"
     gene            complement(1889..2641)
                     /locus_tag="VFQ23_07400"
     CDS             complement(1889..2641)
                     /locus_tag="VFQ23_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906448.1"
                     /translation="MTNSLPLRKPVKWGRLIIGGTLSVLVIAIALLWFAYKNNEPAAL
                     ARIYYVAIYGVNIGDGGVLSGVPCSAPCVFGIQPGETSFDQVLPTLEKNGIARSKCIE
                     EPSVSWYLFTCGAGRLNVQVDTQTNIVSAVWIRPNDPISLGAMIETYGEPNYVTLDQE
                     TPDAIHPRFYWNSMRMVVVVPQIPGNTYDIEKITAVEGVSFSDENLYRLADKETSSYY
                     KPWKGYGMYRAPVEFFPLTPIPTVAMTPSMTP"
     gene            complement(2906..3397)
                     /locus_tag="VFQ23_07405"
     CDS             complement(2906..3397)
                     /locus_tag="VFQ23_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9906449.1"
                     /translation="MLIHAAAGGVGHVAVQLAKWRGAKVIGTASVNLSFLEELGVDQA
                     IDYSTTAFEDVVKDVDVVLDLLGGETQQRSWQVLKPGGMLLSTVQPPSQEIASAHSVR
                     QHMIGTSPPVRKVLTEASKMVENGQLKPHVSAVLQLQDIQKGHQMIEGRHTRGKIVLQ
                     VVQ"
     gene            complement(3417..3842)
                     /locus_tag="VFQ23_07410"
     CDS             complement(3417..3842)
                     /locus_tag="VFQ23_07410"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9906450.1"
                     /translation="MKAVHIHDYGGLDVLHFEEAARPVPAAGEVLIRVHATSMNPFDC
                     AVRAGYMGQYFNYTFPLILGTDVSGVVEEVGAGVTDLAPGDIVYTRTGVYRDGAHAEY
                     ALAIAADVVAKPQSLDHLHAAALPHVTLTAWQGDMRKCS"
     gene            complement(3899..>4105)
                     /locus_tag="VFQ23_07415"
     CDS             complement(3899..>4105)
                     /locus_tag="VFQ23_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906451.1"
                     /translation="QFLSGKRTVFVLRWCTGWLVGAALEPPSRRKLFSVSLVGSPTKP
                     LTLTARYTVYIQKGLYQVNRLVGK"
BASE COUNT         1081 a         1019 c          977 g         1028 t
ORIGIN      
        1 atccaggtcc acggccgaag tgtgaccagt attcccatag gttggattcg agttcctgga
       61 tcaatgttgt cttctcagaa aaatcaatcc cgttcataat caatccttgg tgagatcata
      121 ttcatttcaa cctataaggt atgcaaaccg cctaacggtt ggcagctggc gttagcggct
      181 tggtgggtta ataacctgtc cttttgtgcc ttgcgtaccc gccaatccgt tgcacgtcga
      241 ggatcccctg agcacagcga tgggggcatt gttagcccgc gtttatcctt cacgatgtat
      301 aacttggcag gttaatttcc aaaaacagaa actagacctt tggtgagacc ttctgcactg
      361 atggcgaagt gacccaaatt attcaactgt gcaaattccg cgcgaaagct ggggaacttc
      421 tgtgccttta ggaggtcgaa aagctttttg ctatctagat cgcttggagg ggcagaaata
      481 cacactctcg cggatagatc ttcgtgctgc ttggcgtatg cctcctcaat tttcaaaaat
      541 atatcgtcga atccatccac gatcacatac cgttgaaaga gatgcggttt ttcaaataga
      601 gcaaataaag cgaacactcc accggaagaa tagccccata acgttcgatc atttggcaca
      661 actcgataat tggattccac aaaagggatc aattcttgct gaaagaattc gagatatgga
      721 gctgctcccg gtgtcatgtc attataagga gttggccaaa atatcagatc atgatcacgc
      781 atgttctttc cgccttcatt aggcaacttc ttgctgttgt aagctgggct ggcaataatg
      841 aggtcaggaa tatgttgccc ataaatcagg tattgtacta tatcggtcgc catggcaaac
      901 gcgtgatcgc catctaaaag atagagcacc ggatagagtt tggtagtatt ggcatagtct
      961 tccggcagtc gaatcttcac ctgatattcg cgaccaacga tagaagattt tatgtgacgg
     1021 agttgagaat tcgcaatcgg aacctgtgga tattgagcaa ttgattctgt catgatctta
     1081 actcctatct gagcgggcta acggctcgcg ttagtggcag gtgggtggga caagactcgc
     1141 cgtcgagaca ggaaaaaccc gaagcgagaa aaatgcttga aatgcggcac gtccttcggg
     1201 cagtccccgg cgtctagtgc acgtcgagga tcccctgagc ggagcgatgg gggctgtgtt
     1261 gggcgggttt catcatcata actgattctt caggaagtag atttggcttt gttcatggaa
     1321 tcgatgttgc tatcaaagga gtgaatgttc caggcggggt tgacgtggca acacgaggat
     1381 cgtaggagct acttgattga aacactaaga aagcacaaag aaaaccaaag acaaacccaa
     1441 tactaataaa tgccgcccac catacatggc gagcaccaat acgattgtgg gagattctcg
     1501 ataccattaa cccaacaagc cagatcaggg cgagataaaa caagaaattg accatgaaac
     1561 ctgctggtcg gattcctcta ttgaagacat ctgcaaaatc aatttttccc cagctatcaa
     1621 tcggcgaccc gcctaaccct gcatcgcaaa tgaaaagtgt tggaaagcca gctcctaacg
     1681 cgccgccgca agatggatgc gcaggatacc ggtaattcgt agagacagcc atagcactca
     1741 ttgccaagag gataatgacg acgctgatga aaaaaccctg aaaccctggt cggaagaaat
     1801 gattctcctt catgaaaaga tacctccggt ttcactattc tggtagttga ccacccaacg
     1861 ccgtgttggg tggttttttt ctttttatct acggtgtcat acttggtgtc atcgcgacgg
     1921 taggtatcgg tgtcaaaggg aaaaattcaa cgggcgcccg gtacatgcca taacccttcc
     1981 aaggtttgta gtacgagctt gtctctttgt cagcaagccg atatagattt tcatctgaaa
     2041 aactgacacc ttctaccgcc gttatttttt cgatatcgta tgtgttgccg gggatctgag
     2101 gcactacaac taccattctc atcgagttcc aatagaacct gggatgaatg gcgtcaggtg
     2161 tttcttgatc tagggttaca taattgggtt ctccatacgt ttcgatcatc gcacccaagg
     2221 aaatgggatc atttggacga atccaaacgg cagagacaat gtttgtctgg gtatctacct
     2281 gtacattgag cctgcctgca ccacaggtaa aaaggtacca cgagacgctt ggttcttcga
     2341 tgcacttcga tcttgcaatg ccgtttttct ctaacgtagg caggacttga tcaaatgaag
     2401 tctctcccgg ttgaatgcca aacacacaag gcgcagagca tggtacgcca gaaaggacgc
     2461 caccatcacc gatattgact ccgtatatgg ccacataata gatccttgcc agagcagctg
     2521 gttcattatt cttataggcg aaccagagca aagctattgc gatcaccaag acggatagtg
     2581 ttcctccaat aatcagtcga ccccatttca ccggtttgcg tagtggaaga gaatttgtca
     2641 taaagagctc cttccatcta ctattatgcc tcagaacaca gataggcggg taccgcccaa
     2701 cggtgagcgt tagcggttgg cgggttgaga acccgtcctt ttgacccttg cgtaccggcc
     2761 aatccgctgc acgtcgaaga ctgtcgcaaa gcgacgtgcg cagcgatggg ggctgtgttg
     2821 ggtgcactca ccaaacgccg accttcttgc tcgaaactgc tgaattatac aatttctgac
     2881 ttgcctgact taggttacgc tatggtcatt gaactacttg caagacgatt ttgcctcgcg
     2941 tatgcctgcc ctcaatcatt tggtgtcctt tttggatgtc ttgaagctgg agaactgcag
     3001 aaacatgtgg cttgagctgt ccgttttcca ccattttcga tgcttctgtt agcacttttc
     3061 gaacgggtgg agatgtgccg atcatatgtt ggcgaacact atgagctgac gctatttcct
     3121 gcgaaggcgg ttgcacagtc gaaagcaaca tgccgcctgg tttcagtacc tgccaggagc
     3181 gctgctgggt ttcgcctccc aacaaatcca gcacaacatc tacatccttg acaacatctt
     3241 caaaagcagt cgttgaataa tcgatcgcct gatccacgcc cagctcctcc agaaacgaaa
     3301 gattgacaga ggcagtgccg atcacctttg ctccacgcca cttggcaagt tgcactgcca
     3361 catgacccac gcctccggct gccgcatgaa taagcacggt ttgtccttcg ctaagatcag
     3421 ctgcacttcc tcatgtcacc ctgccaggcc gtcagggtga catgaggaag tgcagctgca
     3481 tggaggtggt ccaaagactg cggtttcgct accacatctg ccgcaattgc cagagcatat
     3541 tcggcgtgcg cgccatctcg atatacacca gttctggtat acacgatatc acccggcgca
     3601 aggtctgtta ccccggctcc tacttcttcg acaaccccgg aaacgtctgt acccaaaatt
     3661 aatgggaacg tgtagttaaa atattgcccc atatagcccg cacgtacagc gcaatcaaaa
     3721 ggattcattg aggttgcgtg aacccggata agcacctcac ctgcagcggg gaccggacga
     3781 gctgcttctt caaagtgtaa tacatcgaga ccaccataat catgaatatg gacagctttc
     3841 atagttcaat tctcctgttc taaaatgatt cgtgaatcgt atgcgcttta aaagcacgtc
     3901 acttaccgac caaacgattc acttgataca accctttttg gatataaacg gtgtacctag
     3961 cggtaagcgt tagcggcttg gtgggagaac caaccaacga aacggaaaaa agctttcgcc
     4021 gagacggcgg ttctaacgcg gcgcctacca gccatccagt gcaccaccgg agaacgaaga
     4081 ccgtcctctt tccggaaaga aactg
//
LOCUS       DASTJP010000245        14942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_806351, whole genome shotgun sequence.
ACCESSION   DASTJP010000245 DASTJP010000000
VERSION     DASTJP010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14942
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806351"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..647)
                     /locus_tag="VFQ23_07420"
     CDS             complement(123..647)
                     /locus_tag="VFQ23_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906452.1"
                     /translation="MKHQPFEEWLLDDQIISPEQKRELDSHLRTCAYCSALAETGMAL
                     RSVKKVSPREGFASRFQVRLAERKVADRKRRLWGSLLFTFGGLAILTWLLFPYLMTFF
                     ASPAAWISVLVEWGVYLITTLRALAEAGTVVVDVIPSFISPFMWMVLLSTIAGIGLLW
                     SVSIWRFVRVPQGL"
     gene            complement(628..1230)
                     /locus_tag="VFQ23_07425"
     CDS             complement(628..1230)
                     /locus_tag="VFQ23_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9906453.1"
                     /translation="MNEELAWVLQAQQGNDEAFTRLVEEHQTHVYNLCYRMLGEPEAA
                     EDAAQESFLRAYQNLHRYDRTRPFATWLLSIAAHYCIDRLRRRKLSMFSLDEENEDGT
                     TYEIADPSSPDPEVESVKREERDRLHILLKDLDATDRAAVVLRYWNDYSEVEIAESLN
                     LTVSAVKSRLHRARRALAGMWQEEKPRARTERRAYETPAF"
     gene            complement(1429..2613)
                     /locus_tag="VFQ23_07430"
     CDS             complement(1429..2613)
                     /locus_tag="VFQ23_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HET9906454.1"
                     /translation="MKNRIVYWAVLFGVFIAFVVFPYFTQVNLPNVSMPSLGSGTARA
                     RVTQIIEDGEIDLGGVVQRYQVARVEVLEGEYKGILMEMDYGKRQVLSNVTYLQEGDD
                     VLVSIGKRPDGVLTVYFADFVRTNSLLWLAAIFAGTILLISRWKGLRSLISMAFSLFV
                     IIGFIIPHILAGEDPVLVSIIGSVILLTVTLYLTYGWTLKTHAAVISMVIVLLITGML
                     ASLAVLFTRLTGSGDENALFLMQMLQTPINLRGLLLGGMIIGALGVLDDLVTTQASAV
                     FELHHANPSFEFRELFRSAMRIGQDHVAATVNTLVLAYAGASLPMLLMFSLSRGDYGY
                     LVNFEFIAAEIVRTLVGSLGLVTAVPLTTAIAIFFALRADSLGEWRQVLGPVGGGDGH
                     SH"
     gene            2798..4150
                     /gene="miaB"
                     /locus_tag="VFQ23_07435"
     CDS             2798..4150
                     /gene="miaB"
                     /locus_tag="VFQ23_07435"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET9906455.1"
                     /translation="MKYHIWTEGCQMNVADSQRVGSSLEHLGYTFTETIEEADVIVLN
                     TCVVRQSAEDKAMGRVTSLRPLKQQNPNLVINVMGCMVGVRGAEKLRQKLPFVDVFSP
                     PSDPGPLVSFLSQGEVRTLETDATARRFAYMDGDLILPQHERGQLVTAHVPIVYGCSH
                     ACTFCIIPFRRGIERSRPVGDIVAEIRSLAAQGVKEVTLLGQIVDRYGKDVPDGPNLA
                     GLLRVVHQVEGIERIRFLTSHPNYFTEELMDTIAELPKVMPHIEVPIQAGDDEVLLNM
                     KRGYNQQEYRDLVAKIRERIPDCSIATDIIVGFPGETDEQFMETYRVLSDLKLDVAHL
                     ARYSSREGTVATRRMTDNITDEEKMRRFRMLEELQEEVVTEINKKYLGETVDVLFEEK
                     VKGRWRGRTPTNKLVFVESNDDLKGKIMPVTVTWTGPWSMQANLAGVHSQPQFELITL
                     "
     gene            4189..4626
                     /locus_tag="VFQ23_07440"
     CDS             4189..4626
                     /locus_tag="VFQ23_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9906456.1"
                     /translation="MNQIRPIALCIFRNSDRILVFEGHDPVKGETFYRPLGGGIEFGE
                     RSEEAVRRELKEELDVEITNLKFIGTLENIFTFNGNSYHEVVLIYDGTLTASELYQQA
                     LLTGKEANGDDIRAIWKNLQEFEAGESILYPVGLLELLRTETS"
     gene            4657..5331
                     /locus_tag="VFQ23_07445"
     CDS             4657..5331
                     /locus_tag="VFQ23_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET9906457.1"
                     /translation="MPQAGLHGLSGLAVRSLAGKREWLLLGIILGTMLPDMDNVAVAV
                     ATVAKMDSHGLHRTFTHSFFFVIAIVLVFYLIGQMRKQPRWANLGLGLGIGVTLHILL
                     DLLGWFNGVYVLWPIDYELNLWRNAQPPAWFMAFMNPAEMLMFAVYLYVLGSWARKYG
                     TDSDFQPKLTNWMIFEIVLFLLFTPLAYVMDSGFLTIFGALYLFSLFMVFFVTIRMRN
                     TVEAAS"
     gene            5422..6225
                     /locus_tag="VFQ23_07450"
     CDS             5422..6225
                     /locus_tag="VFQ23_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET9906458.1"
                     /translation="MRWDELTGDLFPEAVKQAEGVCLLPLSCIERHGPHLTLATDMFI
                     GREVCNRAAALEPSIVFPDFIFTQILEARHYPGCIGIDPELTIRLLENTCREIGRNGL
                     KKIVVVNAHGGNDNMIHYLTQIQLATPRDYVVYVAEQSYSPEDEAAIHAQWDTTVDGH
                     AGEKETSAMLAIRPDLVNSDALRADGEGNPLGRLQHLRDLNIDVGIWWYADHPSHYRG
                     DGTPATAEKGNLWLDARARGLARSIRAIKDDTETKRLQDEFFSRVHKKE"
     gene            6445..7329
                     /locus_tag="VFQ23_07455"
     CDS             6445..7329
                     /locus_tag="VFQ23_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906459.1"
                     /translation="MAVFSLNLQPASATSQVNSKTLVLYEAASGSIPTSPLLSFTDFP
                     FGVAAPIYENQLTILDTTAAGNETYAGWVSTAESITGFPWLDRAAGFQVNFSVGVERE
                     SHANNQRSGFNVILLGNDAKGLELAFWTNEIWVQNDNLTGGLFTHGEGVNIDTTNFTD
                     YQITMSADTYTLSANGTPILSGPVRDYSSFEGFPDPYQTPNFLFMGDNTTSAQARTHL
                     GYVSITGTDPMPVTTTPTVTPVPVTASPVPLTPTTIPPAATPTPTGGGWQSCPPLALA
                     FAFSAIWIKSNRVKRDRF"
     gene            7471..8400
                     /locus_tag="VFQ23_07460"
     CDS             7471..8400
                     /locus_tag="VFQ23_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906460.1"
                     /translation="MSQPMIEAKNLTAAYGSVTALNGVNLRVEQGEIFGLLGPNGAGK
                     STLLACIEGLHKPKQGQVQVGGLSVQSDSAATKRKLGIQLQKTALMDDLTVAELVEVY
                     AALYEVYLSSEQITKLLRQFDLVEKRNTLARRLSGGQQQRLALAVAIANDPQVVLLDE
                     PTSALDPHARRTVWDIVRQLHDEGRTIVLTTHAMEEAEALCGRIAIIDRGQIIACDTP
                     AALIANLQLNSMLKATVELPLDQVRSLPNVSNARYTGQHLEMETAKPEVTLTALHELA
                     DRSGRSIRDVMLRQPNLEDVFLKLTGHALAESN"
     gene            8426..9154
                     /locus_tag="VFQ23_07465"
     CDS             8426..9154
                     /locus_tag="VFQ23_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906461.1"
                     /translation="MKRFLILTKAMTLMHLRNSYVLFWNFAFPVLLMVVYGTVMSQYM
                     DYMTPGVIVLNALSFGLVSSSTMMLEMREKSVLRRLQATPLPASHLIGSYLLVNIIIG
                     ILQSTLIILAGVIIYRVPVSVPSLLLAYPMILTGLLAFMALGGIISGVTAKSGAATAV
                     GMTIYFLLMFISDMIFPLDMLPAWLQKMTAYLPAYPVAQLVRSALLDATLDPKWITNI
                     LLLLAYGIGATLIAARLFRWEPKT"
     gene            9342..10568
                     /locus_tag="VFQ23_07470"
     CDS             9342..10568
                     /locus_tag="VFQ23_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9906462.1"
                     /translation="MTDSHSTHLADSELPMPAQHFELYGRFWDVLSVLIFSFAAAAVL
                     IAHASELTWREWTVAGLSIVQGLLYFFCITRAGWPVSNRGLALYFIAGVSLWVLACWL
                     NPFTWWLGFTYFGQMFGLLPFRAVIAGTAIVTFLIALIISDWKITQIPLGAIVGFTFQ
                     WLGGMAVFLFIYGISSTSRERAKLITKLEAAQKELELARQRDTELATLRERERLARDL
                     HDSLGHALVALSVQLEAVQRLYKVDPERASAQVDELKTLTRASMDDLRRSLAGLRTPG
                     LGDRHLSEALQALSVDVGQRAHLEIKCHVAEEANGLSPALAEALWRVAQEALTNIERH
                     ASARNVALRLDIRDHTASLSVNDDGAGMPVDAEKQTGHYGLRGMRERVEGLGGTLMFS
                     GNGQGGTLLEATLPIL"
     gene            10576..11211
                     /locus_tag="VFQ23_07475"
     CDS             10576..11211
                     /locus_tag="VFQ23_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906463.1"
                     /translation="MADVIQILLVEDQTLMRQGLKTILDLEPGFRVIGEAANGESGVQ
                     MALEMRPDVVLMDIQMPQLNGVEATTQLCRQWPEAKVIILTTFDRDDYVYQGVRAGAL
                     GYLLKDAPANKLIETIRRVHSGEVFIQPEIASRALREMMRPTTTSVEPLSDREREVLV
                     LLAQGASNKEMAEKLFITEGTVKNHVSSILAKLQAENRTQAADIARRHGLV"
     gene            11360..12076
                     /locus_tag="VFQ23_07480"
     CDS             11360..12076
                     /locus_tag="VFQ23_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906464.1"
                     /translation="MKNNQTASFPGVLLYILAVVSGLFLIVISTWGDMEADSYGFARR
                     ASAPLSGLSCPILLTRNESGAIALNVSNTTSTPLHPSARTEISAAFEPEVFVESIELA
                     PGESKRLQWSLKPENIVLGNFVLANVQVYASYPIPNREKTCGILIMDWPGSGLTIVTA
                     LGMFTVIGLGWGLYSMSKSGVLRNKASLTWNAILFLAGLIILGLVFSATGSWIPSIGI
                     LVLSILISILLLSTTVLRGN"
     gene            complement(12138..14831)
                     /gene="acnA"
                     /locus_tag="VFQ23_07485"
     CDS             complement(12138..14831)
                     /gene="acnA"
                     /locus_tag="VFQ23_07485"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HET9906465.1"
                     /translation="MNDFFNARDILRVGDREYVIYRLDALEKAGLATLSKLPFSIRIV
                     LEAALRQCNDSEITQEDVKNLVAWTPNGTRPGIPFLPARVVMQDFTGVPAVVDLAAMR
                     AAVARLGGDPKRINPLVPVDLVVDHSIQVDFFATTDALSRNTEMEFQRNRERYEFLKW
                     GQSAFRNFRVVPPMTGIVHQVNLEYLADVTMVKNDGTSNVLFPDTLVGTDSHTTMVNG
                     LGLVGWGVGGIEAEAVMLGQPMDMLLPDVIGFRLYGKLKDGVTATDLVLTVTQMLRQK
                     GVVDKFVEFYGDGLNSMSLADRATIGNMAPEYGATIGYFPVDAETIRYMKMTGRPKEV
                     VERTEAYMRAQGLFRDDSTPEPEFTDVLELDLDSVVPSLAGPKRPQDRVPMSQLKETF
                     NKALVAPVKERGFELKPEAVASEATFGTNGGSMTMKHGAVVIAAITSCTNTSNPSVMV
                     GAGLVAKKAIEKGLKVKPYVKTSLAPGSRVVTEYLKVANLIDPLAQLGFNIVAYGCTT
                     CIGNSGPLPVEVAKAVTSADLVAAAVLSGNRNFEGRVHPMVKANFLASPPLVVAYALA
                     GTVDIDLDKEPLGEGNDGPVYLKDIWPTQEEIAQTIATSLNVSMFEERYASVFEGSEM
                     WKAINVSGGDLYEWNEASTYIHHPPYFQTLTLDVPAVENIKGARVLCIFRDSLTTDHI
                     SPAGNIATDSPAGKYLQERGVQPKDFNSYGSRRGNDLVMARGTFANIRIKNLLVSPKE
                     GNLTKHFPEGTEMSIYDAAMEYMSDGIPVIVLAGKEYGTGSSRDWAAKGPLLQGVRAA
                     IAESFERIHRSNLIGMGILPLRFMEGENVESLGLKGDEVFDIEGLSDRMAPKTVVNVR
                     ATKPDNTVVEFKATVLLNTDVEVNYYRNGGILHTVLRNLVR"
BASE COUNT         3611 a         3944 c         3747 g         3640 t
ORIGIN      
        1 aatgacacgc ccaccattcg ggccgggtcc ctgtgcatac gccgtccgcg tgggcagcat
       61 gaacaaagcc agaaaagtga gtaacgttag aattctaaaa tttaatttca taagtcacac
      121 tcctaaagtc cctgcggaac acgaacgaac cgccagattg atacagacca taaaaggccg
      181 atgcctgcaa ttgttgacag caaaaccatc cacataaagg gagagatgaa gcttggaatc
      241 acatccacca caaccgtacc ggcctccgct aatgcacgca gggtcgtaat gaggtacacg
      301 ccccactcca cgagcacaga aatccacgcg gcgggcgaag caaagaaggt catgaggtag
      361 gggaacaaaa gccaggtcag gatcgccagg cctccaaatg tgaacaggag cgagccccac
      421 aatcggcgtt tgcgatcggc aactttccgt tccgccaacc ggacctgaaa acgactcgca
      481 aacccttcgc gaggcgatac ctttttcacg gacctcaatg ccatcccggt ctctgccagc
      541 gcggaacaat acgcgcaggt tcgcaggtgc gaatccagtt cgcgtttctg ttccgggctg
      601 atgatttggt catccagcag ccactcttca aaaggctggt gtttcataag ctctcctttc
      661 agtgcgggca cgtggttttt cttcctgcca catgccagcc agcgcgcgtc gtgcacggtg
      721 caatctactt ttgactgcgc tgacagtcag gttcaacgat tcagcaattt cgacctccga
      781 ataatcattc caataacgca gaacaaccgc ggcgcggtcg gtagcatcca ggtctttcaa
      841 caggatgtga aggcggtcgc gctcctcacg tttaacggat tcaacttccg ggtcaggcga
      901 ggaaggatcc gcaatttcgt aggtggtgcc atcctcattc tcttcatcca gagaaaacat
      961 cgagagtttg cggcgtcgca gacggtcgat gcagtaatgg gcggcgatcg aaagcagcca
     1021 ggtggcaaac ggtcgagtcc ggtcataccg atgcagattc tgataggccc gcaggaagga
     1081 ttcctgtgca gcatcttcgg cggcttcggg ctcgcccaac atccggtaac ataagttgta
     1141 gacgtgcgtt tgatgttcct cgacaagcct tgtaaacgct tcgtcattgc cttgctgagc
     1201 ctggagaacc caggcaagtt cctcgttcac tcacgctcct tgtactttct catacgctag
     1261 acgccaaaaa aagttgcagt ttgcgcgtgc gtaattcatt cttcagcacc tttgcaggac
     1321 agaaaagcgt caatttcacc gcgaagagcg ctaagcacgc caagcagaag taatcttcgc
     1381 gcctttagcg ggcttcgcgg ttcaaaattt aaggcaagag aggctggatt agtgactatg
     1441 cccatctcca cctcctaccg gacctaaaac ctgtctccac tcccccagcg aatcagcccg
     1501 gagagcaaag aagatcgcga tggcggtcgt caacgggaca gccgtcacca gaccgagcga
     1561 cccgaccagc gtccgcacga tctccgccgc gatgaattca aaattaacga ggtagccata
     1621 atccccgcga ctcagagaga acatgagcag cattggtaac gacgcgcccg catacgccag
     1681 cactaacgtg ttgacggtcg cggcgacatg gtcctgcccg atccgcatcg cagaccggaa
     1741 caactcgcga aactcaaacg atgggtttgc atggtgcagt tcaaacacgg ccgaggcttg
     1801 tgtggtcacc agatcatcca atacacccaa cgcaccgatg atcattccac ccagcagtag
     1861 gccgcgcaaa ttgatcggcg tttgtagcat ctgcatcaga aataaggcgt tctcatcacc
     1921 cgaacccgtc aatcgcgtga agagtacagc cagagatgcc agcattcctg tgatgagcaa
     1981 aacgatcacc atgctgatca ccgccgcgtg cgttttgaga gtccagccgt aggtcagata
     2041 aagtgttact gtaagcagga tgaccgagcc aataatgctc actaatacag gatcttctcc
     2101 tgccaggata tgagggatga taaatccaat gatcacaaat aaactaaacg ccatgctgat
     2161 caaggaacgc agtcccttcc agcggctgat gaggagaatg gtcccggcga aaatcgcagc
     2221 cagccacagc aaagaattcg tgcgaacaaa atcggcaaaa taaaccgtca gcacgccatc
     2281 tggccgtttg ccaatactca ccagaacatc atcgccttcc tggaggtacg tcacattcga
     2341 aagcacctgc cgtttgccat aatccatttc catcaggatt cccttgtatt ctccttccag
     2401 tacttcgacc cgcgccacct gatatctttg aactacgccg cccaggtcaa tttctccgtc
     2461 ttcaataatt tgcgtcacgc gggcacgtgc agtgccagaa cccagagagg gcatactcac
     2521 attaggtaag tttacttgtg taaagtacgg aaagaccaca aaggctataa aaactccgaa
     2581 tagtacagcc caataaacaa tcctattttt catacgtaga gtatacaccg ttcaagaatt
     2641 cgaccgatat cgtgcatctg tgagagatta taggcttaca gtttgtccgg ccacagagtc
     2701 acacagaaat attaagactc cgcgactttg cgtctccgtg gcaaaagctt tatccccttc
     2761 tttacctgta ctttatctac ggtataattt ctcacctatg aaataccata tctggaccga
     2821 aggttgccaa atgaacgttg ccgactcgca acgcgtcggt tcgtccttag agcaccttgg
     2881 ctatacattt actgaaacca tcgaagaagc ggatgtcatc gttctaaaca cctgtgtcgt
     2941 gcgtcagtct gcagaagata aagccatggg gcgagtcact tccctgcgtc ctctcaagca
     3001 acaaaacccg aatctggtga tcaacgtcat gggctgcatg gtcggcgtgc gcggcgcgga
     3061 aaaattacgc cagaaactcc cgtttgtgga cgtcttttcc cctccctccg acccgggacc
     3121 tctggtctcg ttcctcagcc agggcgaagt ccgcactctc gaaacggatg cgaccgcccg
     3181 ccgcttcgcc tacatggatg gcgacctaat cctgcctcaa cacgaacgtg gacaactcgt
     3241 cacagcgcat gtgccaatcg tctatggctg ttcacatgcc tgtacgttct gtattatccc
     3301 cttccggcgc ggcatcgaac gcagtcgtcc agttggcgat attgttgcag agatccgttc
     3361 gctcgcggcg cagggtgtga aggaagtgac gttgctgggt cagattgtgg accgttatgg
     3421 caaagatgtt cctgatggac ctaatcttgc aggtttgcta cgagtcgtgc atcaggttga
     3481 gggcattgag cgcattcgtt tcctgacatc gcatcccaat tacttcactg aagaactgat
     3541 ggataccatc gcggagcttc ccaaagtgat gccccacatc gaggttccga tccaggcggg
     3601 cgatgacgaa gtacttctca acatgaagcg cggctacaac cagcaggaat atcgtgacct
     3661 ggttgcgaaa atccgtgagc gaattccgga ttgctccatc gcaacggata tcatcgttgg
     3721 cttccctggt gaaaccgatg aacagttcat ggagacctat cgcgtcttat ctgatctcaa
     3781 actggatgtg gcgcatttag ctcgctactc ctcccgcgaa ggaacggtgg ctacgcgacg
     3841 tatgactgat aacatcacgg acgaagaaaa gatgcgccgc ttccgcatgt tggaggaatt
     3901 acaggaagaa gtcgtcactg agatcaacaa gaaatatctc ggtgagaccg ttgacgtcct
     3961 gtttgaagag aaggtaaagg gacgctggcg cggccgcaca cctaccaata agctggtctt
     4021 tgttgagtca aacgatgatc tcaagggaaa gatcatgccc gtcacagtca cgtggactgg
     4081 accgtggtcg atgcaggcga atcttgcggg agttcactcg cagcctcaat ttgagttgat
     4141 cactttataa ttcgtaggtc ggagtgacac tccgacctac atttttttat gaatcaaatt
     4201 cgaccgattg ctctttgtat attccgtaat agtgatcgta ttctggtctt tgaaggtcat
     4261 gacccggtta aaggagaaac attttatcgt ccgctgggag gtggaatcga gtttggggag
     4321 agaagtgaag aggcagtccg gcgtgagcta aaagaggaac tggacgttga gatcacaaac
     4381 ctgaaattca tcggcacgct ggaaaatatc tttactttca acggtaactc atatcatgag
     4441 gttgtgctga tttacgatgg aacgttgacg gcatccgagt tgtatcaaca ggccctgctt
     4501 acggggaaag aagccaacgg agatgacatc cgcgctatat ggaagaacct gcaagagttt
     4561 gaggcaggag aatcgatttt atatcctgta ggactcctgg aattattgcg aactgaaaca
     4621 agttgaacgg ggttatcaat agaaaaggat gtaaacatgc cacaggctgg attacacggt
     4681 ttatcagggc tggcggtccg cagtcttgcg gggaaacgtg aatggctgtt gttgggaatc
     4741 attttaggga caatgctccc ggatatggac aacgttgccg tcgccgttgc cacagttgcc
     4801 aagatggact cgcatggact tcatcgtaca tttacgcaca gtttcttctt cgtcatcgcg
     4861 attgtgcttg ttttttactt gatcgggcaa atgcgtaaac agccgcgttg ggcaaatctg
     4921 ggtttggggc tgggtattgg cgtgacgttg cacattcttc ttgacctgct cggctggttc
     4981 aacggcgtct atgtgttgtg gcccattgat tatgaactca atctctggag gaatgcacag
     5041 cctcccgcgt ggtttatggc attcatgaac ccggccgaaa tgttgatgtt cgccgtctat
     5101 ctttacgtgt taggctcttg ggctcgaaaa tatgggacag attctgattt tcaaccgaaa
     5161 ttaaccaatt ggatgatctt tgaaatcgta ctcttccttt tgttcacgcc gctcgcctac
     5221 gtgatggata gcggctttct cacgatcttt ggagcgttat atctgttctc gctcttcatg
     5281 gtcttctttg tgactatacg gatgcgcaat accgttgaag ctgcaagttg aaatctgctc
     5341 acgccgcctt ttacggcggt aaggatgtcg cccagagcta aagtagcagt taataacatt
     5401 ctacaaagga gattttcacc tatgcgttgg gatgaactca ccggcgatct ttttcctgag
     5461 gccgtcaagc aggcggaggg agtctgcctt ctgcctctat cctgcatcga gcggcatgga
     5521 cctcatctta ccctggcgac cgatatgttc atcggccggg aggtctgcaa ccgtgccgcg
     5581 gctcttgaac catcaatcgt ttttcctgat tttatattta cacagatttt ggaggcgcgg
     5641 cattatccgg gttgtattgg catcgacccc gagctcacca ttcgattact tgaaaacacc
     5701 tgccgtgaaa tcgggcgcaa tggactcaag aagattgtcg ttgtcaatgc gcacggcggc
     5761 aatgacaaca tgatccatta cctgactcag attcaattgg ccaccccgcg cgactatgtt
     5821 gtgtatgtag cagaacagtc ctatagcccg gaggatgaag ccgcgatcca cgcccaatgg
     5881 gatacaacag tagatggtca cgccggtgaa aaagagacaa gcgcaatgct ggcgatccgc
     5941 cctgatctgg tcaatagcga cgcgctgcgg gcagatggag aaggcaatcc cctgggtcgt
     6001 cttcaacact tacgcgatct aaacatcgat gtgggcatct ggtggtatgc cgatcatcca
     6061 agtcactatc gcggggatgg aaccccggcg acggcagaga aagggaacct ctggctggac
     6121 gctcgtgcgc gcggcctggc aagatccatc cgcgctatca aagacgacac cgaaacaaag
     6181 cgcctgcagg atgagttttt cagtcgcgtt cataagaagg agtagtgtag cgtaaggaag
     6241 attcctaaca aacaccttac aatttggcag actacttgta ttggtgttgg atatatgcaa
     6301 taatgatttc gctccgttta gggtgccccc ctcaccctgg atggagctct atatttaagg
     6361 attcttgaat agcgggattt ttggtaaact cattccatga cgaaaaaatt cagtgttcac
     6421 ctatgcgccg ttttattaat attaatggct gtattttcct tgaatctcca acctgctagt
     6481 gcaacgtcac aggttaacag caagaccctt gtcctttatg aggctgcttc cggatccatt
     6541 cccacatcgc cgctgctgag ctttactgat tttccattcg gcgtggctgc tccaatttat
     6601 gagaaccagt tgaccattct ggatacaact gcagctggca acgagacgta tgccgggtgg
     6661 gtttcaacgg ctgaatccat aaccgggttt ccgtggctgg atcgagcagc tggtttccag
     6721 gtaaatttct cagtaggggt ggaacgtgag tctcatgcaa ataatcagcg ctcgggcttc
     6781 aacgtgatcc ttctgggaaa tgatgcgaaa ggtttagaac tcgccttctg gacaaatgaa
     6841 atctgggtgc aaaatgacaa cctgacgggc ggattattca cccacggtga aggtgttaat
     6901 attgatacta cgaatttcac agattatcag atcacgatgt cagcggatac gtacaccctg
     6961 agtgccaacg gcacccccat tctcagcgga cccgtacgcg attacagcag ctttgaaggg
     7021 ttcccggatc cctaccaaac tccgaacttt ctctttatgg gggataacac gacgtccgcg
     7081 caagcccgta cacatctggg atacgtatcg atcacaggca cggaccctat gccagtcacc
     7141 accactccta cagtaacacc cgttcctgtc acagcctcgc cagttcctct cacaccaact
     7201 acaattcctc ccgctgcaac tccaacaccc acaggtggag gttggcaatc ctgcccgcct
     7261 ttggctcttg cctttgcctt ttccgcaata tggatcaaga gcaatagggt aaaaagagac
     7321 cggttttagc cggtctcttt ctttctgttt tcaccatact tatctcacac cacccactat
     7381 gactttcgtc atggtgcaaa tcccgcttct gagactggga gttgccgcag gttacctgta
     7441 acatggggat atcaaaaagg agtatggaac atgtctcaac ctatgattga agcaaagaac
     7501 ctgacggctg cttatggctc cgtcaccgca ctcaatggtg tgaacctgcg tgtggaacaa
     7561 ggagaaatct ttgggttatt gggtcctaac ggcgcgggga agagcacatt gctcgcctgc
     7621 attgaaggct tgcacaaacc caaacagggt caggtgcagg tcggcgggct tagcgtccaa
     7681 agtgactcag cagcaaccaa acgaaagctg gggatacaac tgcaaaagac cgccctgatg
     7741 gacgatttga ccgtcgctga actggtggaa gtgtatgctg cgttgtacga ggtttatctg
     7801 agctctgaac aaattacaaa actgctcaga caatttgatc tcgtcgaaaa gcgcaatacg
     7861 ctggcgcgtc gcctttctgg tggtcagcaa caacgcctgg cactcgcagt ggcaattgcg
     7921 aatgatccgc aggttgttct gctggatgag cccacctccg cgctcgaccc gcatgcccgc
     7981 cgcacggtct gggatatcgt ccgccagtta catgatgaag gccgcacgat tgtcctcacc
     8041 acgcacgcca tggaagaagc agaggcttta tgtggacgca tcgccatcat tgaccgcggc
     8101 cagatcatag cctgcgacac acccgccgca ttgatcgcaa atcttcaact gaattccatg
     8161 ctgaaagcga ctgtggaatt gccgctggat caagtgcggt cgctgccaaa tgtcagcaat
     8221 gcgcgttata ccggccaaca cctcgagatg gaaaccgcca aacccgaagt gaccctgacc
     8281 gctctgcatg aactagcaga ccgctccggt cgctccattc gtgacgtgat gctgcgccag
     8341 cccaatctgg aggatgtctt cctgaaattg actggtcacg cgttagctga gtccaactga
     8401 tgttattgat tgaaaggtat aaaacatgaa acgcttctta attctcacga aagccatgac
     8461 tcttatgcac ctgcgtaata gctacgtgtt gttctggaac tttgccttcc ctgttttgct
     8521 catggtggtc tacggcaccg tcatgagcca atacatggac tatatgaccc cgggtgtcat
     8581 cgtattgaac gccttgagtt ttgggttagt cagcagttcc accatgatgc tcgagatgcg
     8641 cgaaaagagc gtgttgcgcc gcctgcaggc cacacctctg ccagccagtc atttgattgg
     8701 ttcgtatctt ctggttaata tcatcatcgg tattttgcaa agcacgctca ttatcctggc
     8761 tggagtcata atttatcgag ttcccgttag tgtgccgagt ttgctcttag cgtacccaat
     8821 gatcctgact ggtttactgg ctttcatggc gctgggaggc atcattagtg gtgtgactgc
     8881 aaagagcggt gcagccactg cagtcggaat gactatttac ttcctgctca tgtttatcag
     8941 cgacatgatc ttcccgctgg acatgctgcc agcctggctg caaaaaatga cggcttatct
     9001 gccagcgtat cccgtggcac aattggttcg ctctgcattg ctggatgcaa cgctcgatcc
     9061 aaaatggatc acaaacatcc tcctgctatt ggcctatgga attggcgcga cgcttatcgc
     9121 tgcacgactc ttccgctggg aaccgaagac ataagcaata ctatgtcggt ggttgagtag
     9181 cccgaagggc gtatcgaaac taccacttgg ctaagaaaat cttgttatta aaatgttttt
     9241 gatagcatgt tggttgagta gtcctgcgga aatctcgcgg gactactcaa ccaacggcta
     9301 cttcactatt cacttttcct ttaacggagt acactttcga agtgactgat tcacattcaa
     9361 ctcatcttgc tgacagcgaa ctcccaatgc ctgcccaaca ctttgaactg tatggcaggt
     9421 tttgggatgt attgtctgtg ttgatcttca gcttcgccgc agctgctgtg ctaattgcac
     9481 atgcaagtga acttacctgg cgggagtgga cggttgctgg attgtccatt gtgcagggat
     9541 tgctatattt tttctgcatc actcgcgccg gctggcccgt ttcaaaccga ggcctcgctc
     9601 tgtattttat tgctggagtc tcgctatggg tcctggcatg ttggttgaat ccgtttacct
     9661 ggtggctggg tttcacctac tttgggcaga tgtttggttt gttgccgttt cgagccgtta
     9721 ttgctggaac agccatcgtc acgtttctta ttgctttgat tatttctgac tggaaaatta
     9781 cgcagattcc acttggcgcc atcgtcggat ttacattcca atggcttggc ggcatggcag
     9841 tctttctatt tatttatggc attagcagca cgagccgcga acgtgccaaa ctaattacca
     9901 aattggaagc agctcaaaaa gagcttgaac tcgctcgcca gcgggacaca gaacttgcca
     9961 cactgcggga acgagagcgc cttgctcgtg acctgcacga tagccttggg catgcgctgg
    10021 ttgctctttc cgttcagttg gaagccgtac agcgcttgta taaggtggac ccggagcgcg
    10081 cctctgcaca ggtagatgaa ctcaaaacgc tgacgcgcgc cagcatggat gacttgcgcc
    10141 gctccctggc gggcttgcgc acacccggtt tgggagaccg tcatttgagc gaggcgctgc
    10201 aagccttgag cgtggatgtg ggtcagcgtg cgcaccttga gattaaatgt catgttgctg
    10261 aggaggcgaa tgggctttcc cccgcgctgg cagaagcctt atggagagtc gcacaggagg
    10321 cattgacgaa tatcgaacga catgccagcg cacgcaatgt agcgcttcgg ctggacattc
    10381 gcgatcacac tgcatccctt tcggttaatg atgatggagc aggcatgccg gtcgatgctg
    10441 aaaagcagac cggtcattat ggcttgcgag gaatgcgtga gcgggtcgaa gggctgggcg
    10501 gcaccctgat gttttcgggc aatggtcagg gcggaactct tctggaagcc accttaccaa
    10561 ttctataggt caaccatggc tgatgtcatt caaatccttc ttgtcgaaga tcaaaccctg
    10621 atgcgtcagg gactcaaaac catcctggat cttgagccgg gcttccgtgt gatcggtgaa
    10681 gcagccaatg gggaaagcgg cgttcaaatg gcactagaaa tgcgcccgga cgtggtgctg
    10741 atggatatac aaatgcccca gttgaacggc gtcgaggcaa caacacagtt gtgtcgtcaa
    10801 tggcccgaag cgaaggtcat catactcacc acctttgacc gcgacgatta tgtctaccag
    10861 ggtgtgcgtg ctggtgcctt gggatactta cttaaagatg ctcctgcaaa taaactgatc
    10921 gaaacaatcc gacgtgtaca ttcaggcgag gtatttattc aacccgagat cgcatcacgc
    10981 gccctgcgtg aaatgatgcg tccgactacg acaagcgtgg aacccctctc tgaccgcgag
    11041 cgtgaggtgt tggtgctttt agcacagggc gcctccaata aagagatggc agagaaattg
    11101 ttcatcaccg aggggactgt gaaaaatcac gtctccagca tcctcgccaa gctgcaggcc
    11161 gaaaaccgga cacaagccgc agatattgcc aggcggcacg gattggtctg aaacaaatct
    11221 ttaccttgta atcaacctcg ccaatcttct ccatccacac acaagatacc tctgttgcac
    11281 taccaaatta ccgcctctcc agtattgcaa caggcttttc catatcttga aaactgctac
    11341 aattctccca acttaacgcg tgaaaaataa tcaaactgca tcctttcctg gagttttact
    11401 ttatatcctc gccgtggtca gcgggctttt cctgatcgtc atttccacct ggggagatat
    11461 ggaagcggat tcgtacggat tcgcgcgacg cgccagcgca ccattgagtg ggttatcctg
    11521 tccgattctg ctaacgcgca acgagagtgg agcgatcgcc ttgaatgtat ccaatacaac
    11581 aagcacacca ctccatccct ccgcgaggac agagatcagc gccgcgtttg agccggaagt
    11641 atttgttgaa tccatcgagc ttgcgcccgg cgaatcaaag agattgcagt ggtctctcaa
    11701 gccggaaaat atcgttctgg gaaattttgt tcttgccaac gtccaggtgt acgcttctta
    11761 cccgatccca aaccgcgaaa aaacgtgcgg gattcttatc atggattggc ccggaagtgg
    11821 gctgacgatc gtgactgccc tgggtatgtt cactgtgatc ggattgggct ggggcttata
    11881 ttcgatgagc aaatcaggtg ttcttagaaa caaagcctcg ctcacatgga atgcgatcct
    11941 ctttttagct gggctgatca ttctagggct tgttttcagt gcaacaggct catggattcc
    12001 ttccatcgga attttggtct tatccatatt aattagcatc ctattgctga gtaccacggt
    12061 ccttcgcgga aattaattcc gcactgtaaa aaagagacgg tcacctgaag aggtgaccgt
    12121 ctcttcattt ttggaaacta ccttaccaaa tttctcaaca ccgtatgcag aatacctccg
    12181 ttgcgataat agttcacttc aacatcggta ttcaacaaaa ccgtggcttt gaattcaacc
    12241 accgtattat ctggtttcgt ggctctcaca ttgacgacgg tttttggcgc catcctatcg
    12301 cttaagccct cgatatcaaa tacttcatca cctttaagcc caagtgattc gacgttttca
    12361 ccttccataa atctcaacgg caggattccc atgccaatca gattcgagcg atggatacgt
    12421 tcaaaggatt cggcaattgc ggctctcaca ccctgcagta gtggtccctt cgcggcccag
    12481 tctcggctcg aacctgtgcc atattccttc ccggccagaa cgatgaccgg aataccatct
    12541 gacatatatt ccattgccgc atcataaata ctcatttcgg taccctcagg aaaatgtttt
    12601 gtgaggttac cctcttttgg cgacacaagg agatttttga tccgaatatt tgcaaaggta
    12661 ccgcgtgcca ttacaaggtc gttgccgcgc cgcgaaccat acgagttgaa atccttgggt
    12721 tgaacgccgc gctcctgcag gtatttcccg gccgggctat ctgtggcaat gttgcctgca
    12781 ggtgaaatgt gatcggtcgt gagtgaatcg cggaagatac agagcacgcg tgcccccttg
    12841 atattttcta cagccggcac atccagggtc agcgtttgga aatacggagg atgatgaatg
    12901 taggtggacg cctcgttcca ctcgtacagg tcgccacctg agacgttaat tgccttccac
    12961 atctctgaac cttcaaacac actcgcatag cgttcctcga acatgctgac attcagactt
    13021 gttgcgatcg tctgagcaat ttcctcctgc gtgggccaga tatctttgag ataaacaggt
    13081 ccgtcattac cttcaccaag aggttcctta tcgagatcga tatctacggt tccagcgagc
    13141 gcgtatgcaa caactaacgg aggcgacgca agaaaattcg ctttgaccat cgggtggacg
    13201 cgcccttcaa agttacgatt tcccgaaagt acggcggcag caacaaggtc ggcactggtg
    13261 accgctttcg ccacttccac aggaagcggg cccgaattac cgatacaagt ggtgcagcca
    13321 tacgccacga tgttaaatcc aagctgcgcg agcgggtcaa tcagattggc tactttgaga
    13381 tattcagtca ccacacgcga gccaggcgcc agactggtct ttacataagg tttgaccttt
    13441 aatcccttct caatcgcttt ctttgcaacc aaacctgctc cgaccatgac agagggattc
    13501 gatgtattcg tgcaggaggt aatcgcggcg atcacgaccg cgccgtgttt catcgtcatc
    13561 gagccgccgt tcgtgccgaa ggtcgcttcg gaggctaccg cttcaggctt gagctcaaaa
    13621 ccgcgctctt tgacgggcgc aaccagagcc ttattgaaag tctctttgag ttgtgacatt
    13681 ggcacgcgat cctgcgggcg tttgggtcca gccagcgacg gcaccacgga atcaaggtcc
    13741 agttcgagca cgtctgtgaa ttcaggttca ggcgtactgt catcccggaa aagcccctgg
    13801 gcacgcatgt aggcttctgt tcgttcgacc acttcctttg ggcgaccggt catcttcata
    13861 taacggatcg tttctgcgtc cacagggaaa tagccaattg ttgcgccata ttcgggagcc
    13921 atgttgccaa tcgtcgcacg gtcggcaagg gacatagagt tcagaccatc accgtagaac
    13981 tccacgaatt tatcaacgac acccttttgg cgcagcattt gtgtgaccgt cagcaccagg
    14041 tcagtcgcag taacgccatc ctttagtttg ccatacagcc tgaaaccaat tacatcaggc
    14101 agaagcatgt ccatgggttg acccagcatg acagcttcag cttcgattcc accgacgccc
    14161 catccgacca gcccaagccc attgaccatg gtggtgtgtg aatcggtacc cacgagcgta
    14221 tccgggaata atacattgga ggtcccatcg ttcttgacca tggtcacatc ggccagatat
    14281 tccaggttga cctgatgcac gattccggtc attggcggaa ccacgcggaa attgcggaag
    14341 gcactctgcc cccatttgag aaattcgtag cgctcgcgat tgcgttgaaa ttccatttcg
    14401 gtgttgcgac tcaatgcatc tgttgtggcg aagaaatcca cttgaatgga atgatcgacg
    14461 accagatcca caggcacaag cgggttgatc cgctttgggt caccgcccag ccgtgcgacg
    14521 gcggcgcgca tcgcagccag atctaccacc gcaggaactc cggtgaaatc ctgcatgacc
    14581 acccgcgccg gtagaaatgg aatgcccggg cgcgtgccat tgggcgtcca ggcgacgagg
    14641 tttttcacat cctcctgggt gatctcgctg tcgttgcatt gacggagagc tgcctcgagc
    14701 acgatccgga tggaaaatgg aagcttgctc agggtggcaa gaccagcctt ttccaacgcg
    14761 tcgaggcgat agatgacata ttccctgtcc ccaactctta atatgtcgcg tgcattaaag
    14821 aaatcgttca tggctaactc cttgggtgaa atattgccac tccacatgct gcgcatgtgc
    14881 aatgtggctt ttctcaccac tagatcgaga agaatacaaa ggagcgcctt tgataccctt
    14941 gg
//
LOCUS       DASTJP010000246         6169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1128305, whole genome shotgun sequence.
ACCESSION   DASTJP010000246 DASTJP010000000
VERSION     DASTJP010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128305"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFQ23_07490"
     CDS             <1..341
                     /locus_tag="VFQ23_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9906466.1"
                     /translation="ECIVETKRDLEDSSLVATIVGHVGDGNFHMLMLVDPNQPEESAE
                     AARINERLVQRALAMDGTITGEHGVGMGKKKYMQSEHGVEALAVMRSIKRALDPQNLM
                     NPGKMLPEEQ"
     gene            368..916
                     /locus_tag="VFQ23_07495"
     CDS             368..916
                     /locus_tag="VFQ23_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET9906467.1"
                     /translation="MTTYCDYCLSHPEDTFNKTYHDTQYGFPLKDDNLLFERLILEIN
                     QAGLSWITILRKAENFRKAYHGFRIDKVAKYGDKDVARLLADAGIIRNRLKVNAAIEN
                     AKRIQVLRKEFGSFKGWLDAKHPLTKEEWTKLFKKTFVFTGGEIVNEFLMSTGYLQGA
                     HAEHCPVYRKVASLRPAWMMNR"
     gene            980..1756
                     /locus_tag="VFQ23_07500"
     CDS             980..1756
                     /locus_tag="VFQ23_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9906468.1"
                     /translation="MPEIIPTAEPFFLSGDPSKPACLLIHGFTGAPKEMRLMGEYLNR
                     QGYTCLGVRLAGHATHPEDMIRSRYTDWIASVESGYHLLRGITDNIFLMGLSMGGVLA
                     LLTSASLKVRGVVAMSTPSHLPRDYPIWLLHVVSTLMKYSPKRKGEPGSGWFDKTGYQ
                     EQVSYPQNPVRSVAELKKLIIDMNAALPKVTAPVLLIHSKDDTYVLPENLEPIYSGLV
                     NAKDKTKLYITGSGHVVTRDAAREQVFQSASEFIQRVETS"
     gene            1765..2892
                     /locus_tag="VFQ23_07505"
     CDS             1765..2892
                     /locus_tag="VFQ23_07505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9906469.1"
                     /translation="MNRNLIFVASALLFWGFGEGMFFNFVPIYLDNQFDLAKYDIGLI
                     LGAFGFSMAITHIPAGRLADRFGRRPLLVIAWLLGVIATLMMAVADTLPIFLAGLFCY
                     GLTAFVASPLSSYVTAARGAWPISTVLSLTTATFGMGMVLGPVTGGWIGDQYGMRTSF
                     WVAAVIFALSTCFMFLVERQPIDHHDPEAPPPHLLRNRRFVGFMSVLALAIFSMYLAQ
                     PLTPNFLEGVRGLSLSETGLIFTIGALGNSLLAITFSRSNPRIGFLFTQALVILFALL
                     IWKGSSLPVFALGYFLLGGFRAGRPLAMAQARELVHDSQMGVTYGVMETIGSVIFILT
                     PPLAGFLFEQDPTIVYPLAIGLIALSIVVSYLFSPRKEIHA"
     gene            2885..3562
                     /locus_tag="VFQ23_07510"
     CDS             2885..3562
                     /locus_tag="VFQ23_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9906470.1"
                     /translation="MLEIVSFTLGPAQTNAYLVADSETKEAAVIDPSWDGHIILEAAQ
                     KRGWRIGHLWYTHAHFDHIGGAGAIADALNPLPLVALHPNDHVLWRAGGGGAFFGFEI
                     DPGPEPTIDFTHGQILRLGSVEFEIRFTPGHTLGHCILYVANPRAERSETSVERSRSV
                     CFCGDVIFAGSVGRTDLPGGDWNTLEKSIREQIFTLPDDTRLLSGHGPETTVGEEKRS
                     NPFVGGM"
     gene            complement(3604..4794)
                     /locus_tag="VFQ23_07515"
     CDS             complement(3604..4794)
                     /locus_tag="VFQ23_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9906471.1"
                     /translation="MTLIQEKVNQAIEILQEQQTDVWLTFVRETSGVRDPVLDFLIGA
                     NDLTWPSALILTSTGEKIAIIGNLEKDAVQRLNVFSEIIGYDTAVSGPLRDTITRLNP
                     DQIAVNTSKNNVHADGLTHAMYEFLQEYLAGTPYVDRLVSAEPIINAMRGRKTPTELE
                     RIRKAVEITDEIYQKTFDFIKVGMTEIEVGEYMHNLAHEYGVGLAWPAENCPAVNSGP
                     DSPVGHNGPTEIKIERGHLIHFDFGVKYEDYCSDIQRVAYVLREGETEAPPEVQRGFL
                     TIRTAIEKPRESMKAGATGNSIDTMAREIVVDSGYPEYLYALGHQLGRVAHDGGALLG
                     PLWEKYGDSPNQTLEVGQVYTIEPGLTVPNYGYMGIEEDVVMTEKGAEYFTEPQREIV
                     LIRG"
     gene            complement(4791..6095)
                     /locus_tag="VFQ23_07520"
     CDS             complement(4791..6095)
                     /locus_tag="VFQ23_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9906472.1"
                     /translation="MLPLSKVEQFARTHLRYNVTAGLIDGGLFGVAIGFASFGTILPL
                     FVSTMTDSATLIGLVPAIHAAGWLLPQLFTASYTARLRRYKRTVLLSTIHERIPFLGF
                     ALVALFLPSIGLQTGLLLTFLLLIWQGLGGGFTANGWTSMISKIIPPESRGTFFGTQA
                     AVANLFISGAAVGAGYLLDAFDSPTDFMVCFLVASVFFTLSWFALALTREPIDHEKVI
                     EEKPLPFWHGAMNILKRDSNFNWFLVARALSQFATMGFVFYIVYALRRFDMDEVTAGY
                     LTAALTISQTIANASMGWMGDRVGHRAMLIIGAAAATLSSFLAWMAPSLGWFFPIFVL
                     SGFANVSIWTNGMTMTVDFSGENERPFYIGLAQTLTAPATIIAPLIGGWIADTQGFVI
                     TFALSTILSIVMMGILFFLVKEPRKTSRRIPIPKSEEVKETT"
BASE COUNT         1464 a         1510 c         1605 g         1590 t
ORIGIN      
        1 cggagtgcat tgttgaaacc aagcgcgatt tggaagactc atcccttgtt gcaaccatcg
       61 ttgggcacgt cggcgatggt aactttcata tgttgatgct ggtggacccg aatcaaccgg
      121 aggagtctgc tgaagcagcg cgcatcaatg agcggcttgt tcagcgtgcc ctggctatgg
      181 atgggaccat taccggtgaa cacggggtgg ggatgggaaa gaaaaagtat atgcaatcag
      241 aacatggggt ggaagcatta gcggtgatgc gcagcataaa gcgcgcgctt gacccgcaga
      301 atttgatgaa cccgggcaag atgttgccgg aagagcaata agagatttgc catagtaaaa
      361 ttatccaatg accacatact gcgattactg cctctcccac cctgaagaca cgttcaataa
      421 gacgtatcac gacacccaat atgggtttcc cctcaaggat gataacttgc tctttgagcg
      481 attgatcctt gagatcaatc aggcaggttt gtcatggatc acgatcttaa gaaaggctga
      541 gaactttcgt aaggcatatc atggtttccg cattgataag gttgcgaaat atggagataa
      601 ggatgtcgcg cgtttgctgg ctgatgctgg gattatccgt aatcggctca aggtcaatgc
      661 tgcgattgaa aacgccaaac gtatccaggt cctgcgaaaa gaattcggct cattcaaagg
      721 ctggctggat gcaaagcatc cacttacaaa agaggagtgg acgaagctct tcaagaagac
      781 gtttgtcttc actggcggcg aaattgtgaa cgaattcctg atgtctacgg gatacctgca
      841 gggagcgcac gccgagcatt gccctgttta caggaaagtt gcatcgcttc gtccagcgtg
      901 gatgatgaat aggtaatcta taattcaatt gcacaccaca atatattaag cctttaaccc
      961 caacgtctat aaccgattta tgccagaaat tattccaacc gcggagcctt ttttcctgtc
     1021 aggcgacccg tcgaaaccgg catgtttgct cattcatggc ttcacgggcg caccgaagga
     1081 aatgcgcttg atgggggagt atctcaatcg acagggatac acctgccttg gtgtgcgcct
     1141 ggcgggtcac gctacacatc ccgaagatat gattcgatca cgttatacgg attggattgc
     1201 ctcagtcgag agtggatatc atctgttgcg tgggattact gataatattt ttcttatggg
     1261 gctttcgatg ggaggggtgt tggcgctact tacttcggct tccttgaagg taagaggagt
     1321 ggtggcaatg tccacgccaa gccatttgcc acgcgattat ccaatttggt tgctgcacgt
     1381 tgtcagtaca ctcatgaaat acagcccaaa acgaaaaggt gagccaggaa gcggctggtt
     1441 tgacaaaact ggatatcagg aacaggtttc ttatccgcaa aatccagtcc gttcggtagc
     1501 cgagttgaag aaattgatta tcgacatgaa cgctgcgtta ccaaaggtga ctgctcccgt
     1561 tttattaatt cattcaaaag acgacacgta tgtgttgccc gaaaatttgg aaccgattta
     1621 ttcagggctt gtcaacgcga aagataagac aaaactgtat ataacgggat ccggccatgt
     1681 cgtcacgcga gatgcggcgc gtgagcaagt gtttcagtct gcgtcagaat ttatccagag
     1741 agtcgaaact tcctgaggaa aatcttgaac cgtaatctta tctttgtcgc gtctgcattg
     1801 ttgttttggg gctttggcga ggggatgttt tttaattttg tgccgattta cctggataat
     1861 caatttgacc ttgcgaaata tgacatcggg ttgatccttg gggcgtttgg cttttcaatg
     1921 gccatcaccc atatacccgc gggtcgcctg gcagatcggt ttggacgcag gccgttattg
     1981 gtcatcgcct ggctgctggg agtcatcgcg actctgatga tggctgttgc tgatacattg
     2041 cctatctttt tggctggctt attttgttat ggattaaccg ccttcgtggc ctccccattg
     2101 agcagctatg tgactgctgc acgcggggca tggcccatca gcacagttct ctcactgacc
     2161 accgcaacgt tcggcatggg catggtactc ggaccggtga ccggcggctg gattggcgat
     2221 cagtatggga tgcggacaag tttttgggtt gcggcggtta tctttgcgct ttcaacctgc
     2281 ttcatgtttc ttgtggagcg ccagccgatt gatcatcatg acccggaggc tccaccgcct
     2341 catttgctca ggaatcgaag atttgttggt tttatgagcg tgctggccct ggcgatcttt
     2401 tcaatgtacc tcgcacagcc gctcacccca aatttccttg agggtgttcg cgggctatcg
     2461 ttgagcgaga ccggactcat tttcacgatc ggcgcattgg gaaattcgtt gctggcaatt
     2521 actttcagcc gctctaaccc gcgcatcgga tttttattca ctcaagccct cgtcattctg
     2581 tttgccctat tgatttggaa aggctccagt ctgccagttt ttgctttagg atattttctc
     2641 ctgggtgggt ttcgtgcggg gcgtccactt gcgatggcac aggcccgtga acttgtccat
     2701 gattcgcaga tgggcgtgac ctacggcgtc atggaaacga tcggttcggt gatttttatc
     2761 ctgaccccac cactggcagg ctttctcttt gaacaggatc caaccattgt ctatccgctt
     2821 gcgattggat tgatcgctct ctccattgtt gtcagttatt tgttttcccc acgaaaggaa
     2881 atccatgctt gaaatcgttt cctttacact tggccccgca cagacgaacg cttacctcgt
     2941 cgccgattct gaaacgaagg aagccgcggt gattgatcca tcctgggatg gtcatattat
     3001 tttggaagct gcgcaaaaac gcggctggcg gatcggtcat ctttggtaca cgcacgcgca
     3061 cttcgatcac atcggaggtg caggcgcgat tgctgacgcg ttgaatccgt tgcctttggt
     3121 agccttacat ccaaacgatc atgtactctg gcgggcaggc ggaggaggag ctttctttgg
     3181 ctttgagatt gacccgggcc ccgaaccgac gattgacttt actcatggac agattttgag
     3241 attggggagt gtggaatttg aaattcgctt cacgccgggg catacacttg gtcactgcat
     3301 cctatatgtt gctaaccctc gcgctgagcg aagtgagacg tcagtcgagc gcagtcgaag
     3361 cgtatgcttc tgcggtgacg tgatcttcgc gggttcagtc ggccgcaccg atctgcccgg
     3421 gggagattgg aatactcttg aaaagagtat cagggagcaa atatttacac tgcctgatga
     3481 cacgcgtttg ctttcaggtc acggtccaga aactacagta ggggaggaga agcgaagtaa
     3541 tccgtttgtg ggtgggatgt gaatgtaaga cttccgaatt cttcaagaat tcggaagtct
     3601 tttttaacct cttatcaata caatctctct ttgtggttcg gtaaagtatt ccgcaccttt
     3661 ttccgtcatc accacatctt cttcaatccc catatatccg taatttggaa ctgtaagacc
     3721 tggctcaatc gtatacactt gcccaacctc aagcgtttga ttgggcgaat caccatactt
     3781 ttcccacaag gggcccagta gcgcgccgcc atcgtgagcg acacgtccca gttggtggcc
     3841 taaggcgtaa aggtattccg ggtacccgga atctacaacg atttcgcgtg ccatcgtatc
     3901 gattgagttt cccgttgcgc cggctttcat cgactcgcgc ggtttttcaa tggctgtgcg
     3961 aatggtaagg aatccgcgtt gcacttcggg tggcgcttcc gtttcacctt cgcgtaacac
     4021 ataagccacg cgttgaatat cagagcaata gtcttcatat ttcacgccga aatcaaagtg
     4081 gatgagatgt ccacgttcga tcttgatctc cgtggggccg ttgtgtccga cgggcgaatc
     4141 gggtccggag ttgaccgcgg gacaattctc agcaggccag gcgagcccta ctccgtattc
     4201 atgggccaga ttgtgcatat attcgccgac ttcgatttcg gtcataccca ctttgatgaa
     4261 gtcaaatgtc ttctgataga tctcatcggt aatctcaaca gctttgcgaa tccgctccag
     4321 ttctgtcgga gtttttcgcc cgcgcatggc gttgatgatc ggttccgcgc tgacgagtcg
     4381 atccacatag ggtgtgcccg ccagatattc ctgcaggaac tcatacattg catgagtcaa
     4441 gccgtccgca tggacgttat ttttcgaagt gttgacagca atctggtccg ggttgaggcg
     4501 tgtgatcgtg tcacgcagag gtccgcttac cgctgtgtcg tatccgataa tctcactaaa
     4561 tacgttgagt cgttgaacgg catctttttc cagatttccg ataatggcga tcttttcacc
     4621 tgtgcttgtg aggatcaatg ccgatggcca ggtaagatca ttggcaccaa ttaagaaatc
     4681 aaggactggg tcacggacgc cgcttgtttc acggacgaat gtgagccaca cgtctgtctg
     4741 ttgttcctga aggatttcaa tcgcctgatt gactttctcc tgaatgaggg tcatgtggtc
     4801 tccttgactt cttctgactt tgggatagga atgcggcgag aggtcttccg tggctctttc
     4861 acgagaaaaa acagaatccc catcattaca attgataata ttgtagaaag ggcaaatgta
     4921 atcacaaagc cctgtgtatc tgcgatccat ccgccaatca agggagcaat aatcgtagcg
     4981 ggcgcagtca gcgtttgtgc cagcccaatg taaaaaggac gctcgttttc tccgctaaag
     5041 tcaacggtca tggtcatgcc gttcgtccag atcgaaacgt tcgcaaaccc tgataaaaca
     5101 aaaatcggga agaaccagcc cagcgatggc gccatccagg caaggaacga acttagtgtt
     5161 gccgccgccg cgccgataat cagcattgcc cggtgaccga cacggtcacc catccagccc
     5221 atactcgcat tcgcgatcgt ctgtgaaata gtaagcgcgg cagtcagata ccctgcggtg
     5281 acttcatcca tgtcgaatcg gcgcagcgca taaacaatat aaaaaacgaa tcccatcgtc
     5341 gcaaattgcg acagcgcgcg tgccaccaga aaccagttga agttgctatc gcgcttgaga
     5401 atgttcatcg ctccatgcca gaaggggagt ggtttctctt cgataacttt ttcatgatca
     5461 atcggttcac gcgttaatgc cagcgcgaac cacgaaagcg tgaagaatac actggccacc
     5521 aggaaacaga ccataaaatc cgttggcgag tcaaacgcat ccaacagata acctgctccc
     5581 actgccgcgc cgctgataaa cagatttgcc actgcagcct gggtgccgaa aaatgtcccc
     5641 cgtgactcgg ggggaatgat cttggaaatc atgctggtcc agccgttggc cgtaaaaccg
     5701 ccgcctaacc cctgccagat gagcaggaga aacgtaagaa ggagaccggt ttgtagaccg
     5761 atggagggca ggaacaatgc aaccagcgcg aatcccaaaa atggaatacg ctcatggatg
     5821 gtcgataaca ggacggttcg cttatagcgc cgcagacgtg ctgtataact cgccgtgaag
     5881 agctggggca ggagccaacc agccgcatgg atagcaggaa caagcccaat caaagtggct
     5941 gagtcggtca tggtcgagac aaacagtggc aggatggtgc cgaaggatgc gaaccctatc
     6001 gcgaccccga acagtccgcc atcgattaag ccagcggtaa cgttatagcg caaatgagtc
     6061 cgagcaaatt gttcaacttt ggagagggga agcatgatct gattctactc tagtgtttga
     6121 tttcttcgta cttcctctcg taaatcattc tcctaaaccc gattatctt
//
LOCUS       DASTJP010000247        20472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1162332, whole genome shotgun sequence.
ACCESSION   DASTJP010000247 DASTJP010000000
VERSION     DASTJP010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162332"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFQ23_07525"
     CDS             <1..674
                     /locus_tag="VFQ23_07525"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9906473.1"
                     /translation="IYFAVWIIFFDRFKEVRGWGISDMYITYGVLASAFGLVAMLFGN
                     AFTLSEIINLGRLDYYLSLPRPVLLHTVSSRMISSGMGDFTYGFVSYALSGQYTWDGF
                     LRFILATLLAAAVFASFLILVQSLAFWFGAMSNLTNLALNAMITFGIYPTTLFDNYAK
                     LILFTLIPAAFMGAVPAEFVRAYTWQSLAQLLIGAVVFLFLAVTVFRLGLKRYESGSA
                     IQVEV"
     gene            complement(858..1643)
                     /locus_tag="VFQ23_07530"
     CDS             complement(858..1643)
                     /locus_tag="VFQ23_07530"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9906474.1"
                     /translation="MNPFKLAWTYLRIGVMNELQYRVNFFIQLLQSAVALTTGLIGLS
                     LVFGQVNNLAGWSRAELLAVMGVHLLMGGVVRSAIQPNMERLMDDVLNGTLDFALTKP
                     ADAQALVSVREFRFWQLTDVLVGLVVIGVAVTQLQAGIGLLQVLAFILALMLGAIMLY
                     CVWLMVTSIAFWVIRVSEIVNLFEGLYAAGRWPVGVYPDWLRTGLTFLVPVAFAVTVP
                     AEAMTNRLTPETMLFAFGLTVMFVILARSIWLIGLRSYSGASA"
     gene            complement(1652..2455)
                     /locus_tag="VFQ23_07535"
     CDS             complement(1652..2455)
                     /locus_tag="VFQ23_07535"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9906475.1"
                     /translation="MRRYIDFYLSSMKVSILQQFQYRVANYFYMIGMIAEPVIYLVVW
                     STIANQQGGSAGGYTPGEFAAYYIVWTLVRNMNIVFTPYGWEWRIRQGNLSMELMRPM
                     HPIHNDIAYFAGWKFVVIILWLPLALFLSWVFKPTFDITWIEGIAFFVAIWGAYLIRT
                     MLLSLLGMITFWTTRVSAIFELYFGLELILSGRLVPMTLMPQWVQNLSNYLPFKWTFY
                     FPIETLVGKMTTQELATGIGMQILWIVISATILNFVWRGAIKQFSAVGG"
     gene            complement(2452..3432)
                     /locus_tag="VFQ23_07540"
     CDS             complement(2452..3432)
                     /locus_tag="VFQ23_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9906476.1"
                     /translation="MAPVIKISQLAKHYQVPEREGGLKAAITSLVKRKYRTVKAVNEI
                     SFNIEPGEVVGFLGPNGAGKTTTLKMLSGLLHPTSGEVRVLGFEPFRRSYDYLRQITL
                     VMGNRNQLAWDLPALDSFDLQRAVYGMPADDFKRTRDEFIDLLELKDLVTKPVRNLSL
                     GERMKMEIVAALLHKPKVLFLDEPTIGLDVTMQRRIRTFIAEYNRRNNATVLLTSHYM
                     ADVEALCRRVIVIHHGRILFDGDLSNLVNQFSSYKTLSVTLQNPETELSQYGEVLSRD
                     DGRVTLRVPKAQTSQVTARLLSDLQVDDLTVEDAPVDDIIDQVFSQKEDV"
     gene            3836..4108
                     /gene="rpsO"
                     /locus_tag="VFQ23_07545"
     CDS             3836..4108
                     /gene="rpsO"
                     /locus_tag="VFQ23_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET9906477.1"
                     /translation="MPLQKEIKTKLITDYHRHDADTGSPEVQIAILTGRIQQLTDHLR
                     INKKDQSCRRGLLKLVGQRRRTLTYLRKVDYQRYLAVTESLNLRHK"
     gene            4277..6565
                     /locus_tag="VFQ23_07550"
     CDS             4277..6565
                     /locus_tag="VFQ23_07550"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET9906478.1"
                     /translation="MKPESKKYTTTVGRHTLTFETGKLAGQAGGAVTFGTPESIVFAA
                     ATMGGVREGIDFFPLSVEYEERLYAGGRIPGSFFRREGRPGTEAILVARLTDRPLRPL
                     FQDGMRNEVQVIMYALSSDGVNPLDILAINAASAAIMISDIPWGGPVGAVRVGRVNGE
                     FVINPTFAEIETSDLDLRIAGTREAILMVECGANEIPEDVMVQALELGHKSLQPLIDL
                     QFQMQAEVGKPKREVTLSLPDADLRNKVFERVNGPMTELLEKPLTKLEFYTGMTELKE
                     MVVAEMCFVPEGASPSDYPAVNSVREAFEQAEQAIVRERILDEGKRPDGRTPTDIRPI
                     WCEAGLLPRAHGSGLFTRGETQILSVATLGTLGEAQELDNLSPAKDKRYMHHYNFPPF
                     STGEVKNIRGQSRREVGHGALAERALEPVIPAEESFPYTIRVVSEALSSNGSTSMGSV
                     CGSTLALMDAGVPIKAPVSGVAMGLITDDSGRYKILTDIQGTEDHLGDMDFKVAGTAA
                     GITALQMDIKISGLSAQMMKEALEQANIARMSILEKMLAVLPEARAELKEHAPRIITV
                     KIPVDKIGALIGPGGKNIRSLQEETGTKIDIEEDGTVYIASTNAVGAKIAQERVAGLG
                     ENAVIGNIYTGKVVRIADFGAFVEILPGVDGLVHISQLDSERVNRVEDVVGMGDEITV
                     MVTDIDPQGKIRLSRQAVLEGWTAEEAREKDGPRRSGGGNRGGDRGGGRGGDRGGGRG
                     NDRGGFRGGNRSGGDQNRGGRN"
     gene            6879..7814
                     /locus_tag="VFQ23_07555"
     CDS             6879..7814
                     /locus_tag="VFQ23_07555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET9906479.1"
                     /translation="MKAVRIHEYGGHDVLVYEEAPKPSPRVGEVLIKVHASGVNLFDC
                     AVHAGYMHPFFNYTFPLVIGTDVSGVVEEVGEGVTDVSPGDAVYTRAGVFQDGGNAEY
                     VVAPAANVAPKPQTIDHIHTAAIPHVGFAAWYALVDLAQISEGQSVLIHGAAGGVGHI
                     AVQLAKLRGAKVIGTASVNLDFLHELGVDEAINYSTTSFEDVVKDVDVVLDLIGGETQ
                     ERSWKLIKPGGILVTTQTYPPPEEVAIAHGVRLGHVNQNPPMRMVLTEYAKMIDAGQL
                     KPHVSTVLPLAEVQKGHELISTMHTRGKIVLQVVD"
     gene            8054..9172
                     /locus_tag="VFQ23_07560"
     CDS             8054..9172
                     /locus_tag="VFQ23_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906480.1"
                     /translation="MKRFFVFLVPFATLFFVLLAITATANASGNFQTDVLEQGKYIAT
                     IGGCIDCHTPLEDEFQDPTQMTPEQLKVLSFNLGAALDQENKLLAGGRFFDLGPAGVL
                     LTRNLTADQESGLGSWTDEQIKLAIKTGINNEGTLLFPVMPYHVYNTMADSDLDALVA
                     YLRTVRAIDNSIPKNTISTQGFPIPSYETGIIAPDPSDKAARGAYLVNSVMACTDCHT
                     PVDPATGAPQMEKYLAGRQPYEGPWGIVYGGNITPDNETGIGSWTEDEIIRAITTGVG
                     KDGRRLILMPWFNYVALKPEDAQAIAYYLKNVLPAVDNEIPAASLNKGFEEIVPGVQN
                     QSTAGELMSSPVILVIVGIGVILAVSLIASSSKRNQPK"
     gene            9172..11064
                     /locus_tag="VFQ23_07565"
     CDS             9172..11064
                     /locus_tag="VFQ23_07565"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9906481.1"
                     /translation="MTQAINRLLKEKYLNIQAKLVFPLAITIILLVVVLSPFTNRLIT
                     NRIEDEADRRLSEIAGSVGALIENSEALARNNAALLAEQPDVVNAFSDLSSSTQSLNA
                     SKVSLDLQELILYKADFKPGDQAYFYGGPAVARRLQVSEDINDIRDELILSALESKAL
                     NSSVAIAPQSSQIIGVAPVFAAEGGDLLGVVVATFYMDQSYIDGISQIINTDVAIVKD
                     NSVIVSTIDQNAGIERLINQGWLNSSETPSQNVTYSTNEDFRLLGHPLVISGNQQGSV
                     IVAQPIDNLFAVSRNIQLILGTVTGIFGITALWFWIAAFLAFTRPLAQLTQATTNMSQ
                     GDLNQKVNPSYVLFKDEITILSENFNAMAANLSDSYASLEEKVEQRTHELADARDEAV
                     AANKSKSEFISVVSHELKLPMTSIKGYSDLMLAGATGPLNENQTSFLTTIRNNVNRMA
                     TLVSDLADISRIESGNLRLEARSVPVWDVIDEVVTLTKTQVTEKNQVVTVDIPTELPK
                     AWCDRNRLAQVLTNLVSNANKYTPEGGAIVVQAIRKNGMIQVKVEDNGHGMTLEDQAK
                     LFTKFFRSADDKIREAPGTGLGLSITKNLIELQGGKVWFESEFRKGTSFYFTVPISLD
                     GNTPPP"
     gene            11269..11505
                     /locus_tag="VFQ23_07570"
     CDS             11269..11505
                     /locus_tag="VFQ23_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9906482.1"
                     /translation="MLPGLTAREQQILDLIAEGLTNRQISSNLYISESTVENHIHHIF
                     VKLGISNRAQAVAHAFQLKIRLPTDIFENEGNPS"
     gene            11570..12643
                     /locus_tag="VFQ23_07575"
     CDS             11570..12643
                     /locus_tag="VFQ23_07575"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9906483.1"
                     /translation="MLIIDSHLDLAWNALQWNRNLQQSVYTIRTQEGNLTGPGRGQGT
                     VALPELRNGRVAVCFATLLARSTGKIEPGLDYQSPLQAYAAAQGQLSYYQALTESGEV
                     RLIKNLSDLDNHMLEWGQWENDPQSTHPPIGLIISMESADPILKPDQLSAWKEAGVRI
                     IGPAHYGPGRYAGGTSTESGLTPDGFALLSEMDRLEIILDLTHLSDQAFWQALDHFGG
                     IVIASHNNCRSLVPHQRQFDDEQIKAILARQGVIGAALDNWMLTPNWVRGARDNPPVT
                     LENVADHIDYICQLAGNAEHAAIGSDLDGGFGREQSPTDLDTIADLQKFVEILTGRGY
                     RNEDISLIMHGNWLRLLRGAWVG"
     gene            12837..13220
                     /locus_tag="VFQ23_07580"
     CDS             12837..13220
                     /locus_tag="VFQ23_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HET9906484.1"
                     /translation="MPKQQITTNTGASPMGAYSQGLRAGDFIFVSGQGPIDPDTGKIV
                     GDTIEEQTARTLDNLKAILEAGGATMADVVKVTAHLTDLANFAAYNKVYATYFPDPKP
                     VRTTVGSQLLGMLVEIDAIAYVGEK"
     gene            13224..14273
                     /locus_tag="VFQ23_07585"
     CDS             13224..14273
                     /locus_tag="VFQ23_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HET9906485.1"
                     /translation="MRVDELETPVPVVDMDRLQSNISRLQSYLDEQGIANRPHIKTHK
                     IPAIAQMQMDAGAIGITCQKVSEAEVMADAGFTDIFIPYNIIGESKLQRLMSLASRIT
                     VSVTADSAYVIRGLSEAAQQAGLTLPVLIECDTGAHRCGVQSPQEAADLARLINQSPN
                     LHFGGLMTYPTSEETDKFVRETRQLLAEDNLQIERVSGGGTPTMWQVHTLREVTEHRA
                     GIYVYGDRLTLRSGAVTLDTCALKVHTTIVSRPTAERGILDAGSKSLSSDLHGLDGYG
                     YICEYPYAKIYALSEEHGHVDFSACERKPEIGERVTVIPNHCCTVTSQFDEVVGARNG
                     EVEVIWQVTARGTVR"
     gene            14274..14924
                     /locus_tag="VFQ23_07590"
     CDS             14274..14924
                     /locus_tag="VFQ23_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET9906486.1"
                     /translation="MSAIETASEAIKQSGIIAILRGDFSVEDMLRIGKALLQGGVTVM
                     EVTLNSPAALEALPKLRKQFEDEMLVGAGTVRNADHAKSAWDSGAQFLVSPNLDVDTV
                     SFARTKDLLHLPGVFTATEAQTAFAAGCRMLKLFPMEAFANGPAFLKALRAPLNDISF
                     VPTGGISLENIADYARAGAVAVGLGSKLVLSREQSSNDLAARAKALRQTWEQARYA"
     gene            14917..15879
                     /locus_tag="VFQ23_07595"
     CDS             14917..15879
                     /locus_tag="VFQ23_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET9906487.1"
                     /translation="MPEPRFDVTSIGEMLLRLSVPSGERLEMARQLDVYPAGAEANVV
                     SLLARLERKTYWAGALPENSPGQLAANALRLSGVDTSGIIWNEAGRIGTYYVEFGEPP
                     RGIQVTYDRADSCMSHLKADEIDWDALLDTRLLHLTGITPALSSSCREIVERALDEAR
                     QRSVPVSFDVNYRGKLWTKTDAHNILSPMIQQVELLFCSARDAIALFNCTGTFDEIAQ
                     KLLELSQAKFVAVTNSNQGALLWDGKEWLHEPARSTKIIDRLGAGDALAGGVIHGWLD
                     GDLRVGLRYGVTLAALALSQFGDMVITNKDELLALSQGDSTLTR"
     gene            16008..16727
                     /locus_tag="VFQ23_07600"
     CDS             16008..16727
                     /locus_tag="VFQ23_07600"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9906488.1"
                     /translation="MEVQIAVAKVNKYATSESGDTLEVVERPNGGLSVVLADGQTSGR
                     GAKAVSQLVVRKVIGLLAEGVRDGAAARAASDALYTDKQGKVISTLNIASVDFYSGTI
                     VLTRNNPSPMFICRGDQIDCRDEESIPLGTSRNVRPLITEINIEPRLIIVIYTDGLTH
                     AGKRRGQPMDVRITLRSILDGQDLSPQTIADSLLAEAVSLDDNRPADDISVVVLKVTA
                     RSGDNVRRMTVRLPIEGNSRL"
     gene            16724..17755
                     /locus_tag="VFQ23_07605"
     CDS             16724..17755
                     /locus_tag="VFQ23_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET9906489.1"
                     /translation="MKLVWRQWKAAWRDTLILINEFRTPLIVFTLIVIGGGLYYRALA
                     GRLGEPIQSVGEAIFIILSAAFLQIVGEFPQHPYLQLFHFFMPLIGAIVLAQGLADFG
                     SLLFNRHTRSKEWEMAVASTLNNHIILVGLGHLGYRIVQKLNEMGREVAVIELNPSVD
                     SFKAVQKMGVPVIQDDGTRVATLDAANIKDARTIILAGQNDAMNLQIALKARSLNPNI
                     QVVIRIFDVDFAHALHDQFGFVALSATEMAAPVFAAAAAGADVTNPISIEGQQLSLAR
                     MTISPSSRLANKTVGYVEDNYRLNIILLRHGQDSEMHPTDTRSLQAGDTLAVLGGPEQ
                     LRQLMQDTR"
     gene            18224..19729
                     /locus_tag="VFQ23_07610"
     CDS             18224..19729
                     /locus_tag="VFQ23_07610"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET9906490.1"
                     /translation="MRKLLISLLTFSLFLLSCSMPQPSATPPSVERTPDPLQTPWEDR
                     SIFKEGLVKSQQSVLDELDGASIYHIEFNISDDLLHIAGTQDVRYTNNEDVAFDAVYF
                     HSFPTILGGQMVISSTRINDTEADFSFEFQNSVFRVPLQSELLPGQSTIIHIDFAVTV
                     PQTLESNYGMLAYAEDVLALAHAYPLIAVYDDEGWNIEPPSQNGDVTYADAAFYIVRI
                     NAPKELTLVTSGHQISLREDDQLQSLVVASGPARDFFLAASPKYEEISQTFGEITVRS
                     YAPKESRDGSQLALDVAARAIEDFSKRYAPYPYTEFDIASTPTLALGIEYPGLVVITS
                     EIYDLNRNIRGAPARVYLESTVAHEVGHQWFYNLVGDDQLDDPWIDESLTQFVTLQYY
                     MDAYGTAGANGFRASLEERWARVDKADIPIGLSVASYEGPEYGAIVYGRGPLFFVALK
                     DVMGAENFDNFLKAYTETFSWKVATPEGLQSLAEKQCACDLDSIFNEWVYP"
     gene            19840..>20472
                     /locus_tag="VFQ23_07615"
     CDS             19840..>20472
                     /locus_tag="VFQ23_07615"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906491.1"
                     /translation="MIRNLYTRLALIAVIAALAIWVDLTDVVRLRNPLNDDIVAERNI
                     TPRLGLDLRGGLQVLLEADLPADTPVDAEAMETARSIVENRTNALGVSENLIQLAGDR
                     RIVGEFPGAEDADAILDIIQQTGLLEFVDTGDFRPEEGTVLQTDFATGSSTEPAVTPT
                     PTTDGTTPPTVYHTIMTGKELESVNVSAGQVGGAVIDFTLSPEGTTIFGQH"
BASE COUNT         5281 a         5000 c         5237 g         4954 t
ORIGIN      
        1 tcatttattt cgcggtttgg atcatcttct tcgaccgctt caaagaagtt cgcggctggg
       61 gaatcagtga catgtatatt acctacggtg tactggcaag tgcatttggt ctggttgcca
      121 tgttattcgg caatgccttt acactgagcg aaatcatcaa cctgggtcgc cttgattatt
      181 atctatcgtt accgcggccg gtattactcc acacggtctc gtctcgcatg atctccagtg
      241 gcatgggtga cttcacctac gggtttgtca gttacgcgtt atccggtcaa tacacgtggg
      301 atggtttcct gcgcttcatt ctcgcgacgc ttttagccgc tgccgttttc gcctcgtttc
      361 tgatcctggt gcagagcctg gccttctggt ttggggccat gtccaatctg acaaacctgg
      421 cgctcaacgc tatgatcact tttggaatct acccaaccac cctatttgac aattacgcga
      481 agctgattct atttacactg attcccgcgg cgtttatggg cgcggtccct gcggagtttg
      541 tacgcgccta tacgtggcaa tcgctggctc agcttctgat cggcgctgtg gttttcctct
      601 ttttagcagt gacagttttc aggctgggac taaagcgata tgaatcaggc agtgccatac
      661 aagtcgaggt gtagcacaag aacatgctgc aattgacagt gttcgcaata atgctgttta
      721 gttacgcatc agcaacgttt gtgtcggtga catttttgga tcgatattgt cgggaaggtc
      781 tggtgagatg gtacgaaccg accatgaaca acaaaaagcg actctcatcg agtcgctttt
      841 tgtttgtgca aggctatcta agcagatgca cccgagtagc ttcgcagtcc aattaaccaa
      901 atgctgcgtg ccagtatgac gaacatgaca gtgagaccaa aggcaaacaa catcgtctcc
      961 ggcgtcagtc gatttgtcat tgcttctgca ggcactgtca cggcgaaggc aactggcaca
     1021 agaaacgtta ggcctgtgcg cagccaatca gggtagacac ccacaggcca acgtccagcc
     1081 gcgtagagcc cttcaaagag attcacaatt tcactcacgc ggatcaccca aaaagcaata
     1141 gacgtgacca ttagccagac gcagtacaac atgatcgcgc cgagcatcag cgcaaggata
     1201 aacgcgagga cctgtagcaa tcctattccg gcttgaagct gtgtgaccgc aactccaatc
     1261 acgaccagcc caaccagcac gtccgtcaat tgccagaagc gaaattcgcg cacactgacg
     1321 agtgcttgag catctgccgg cttggtgagg gcgaaatcca gggtgccgtt caacacatca
     1381 tccatcaggc gctccatgtt tggctgaatc gcagagcgga cgacgcctcc catcaaaaga
     1441 tgaacaccca tcacggcaag caattccgcg cgtgaccacc cggccagatt attcacctga
     1501 ccaaagacca gtgaaagccc gatcaggcct gttgtcagtg cgacggcaga ttgaaggagc
     1561 tgaataaaga aattgacgcg atattgcagt tcgttcatca ctccaattcg caaatatgtc
     1621 catgcgagtt taaacggatt catggtatct cctacccgcc cacagcggag aactgtttaa
     1681 tggcgccgcg ccaaacaaag ttcagaatgg tagcactgat tacgatccag agaatctgca
     1741 ttccaatacc tgttgcaagt tcttgagtcg tcatctttcc gaccaatgtt tcaattggaa
     1801 aataaaaggt ccacttgaac ggtaaatagt ttgagagatt ttgcacccac tgcggcatga
     1861 gagtcatcgg cacgagacga cccgagagaa tcaattcgag gccgaagtac agctcaaaaa
     1921 tcgcgcttac ccgcgtggtc cagaaggtga tcattccaag tagcgatagc agcatggtgc
     1981 ggatgagata agcgccccag atggcgacga agaaggcaat accttcgatc caggtgatat
     2041 caaatgtcgg cttaaagacc caggagagga acaatgccaa cggcagccag agaatgatga
     2101 caacaaactt ccagcccgcg aagtaagcaa tatcattgtg aattgggtgc atgggacgca
     2161 tcaattccat cgagagattt ccctggcgga tgcgccactc ccagccataa ggtgtgaaga
     2221 caatattcat attgcggacc agagtccaga cgatgtagta ggctgcaaac tcgcccggag
     2281 tatatccccc ggcgctccct ccctgctggt ttgcaatcgt ggaccaaacc acaaggtata
     2341 tgaccggctc ggcgatcatg ccgatcatat agaaatagtt cgccacgcga tactgaaact
     2401 gctgaaggat agagaccttc atggaggaaa gataaaaatc gatgtagcgt ctcatacatc
     2461 ctccttttgg gagaagacct ggtcaataat gtcatcaacg ggtgcatctt ccacggtgag
     2521 atcatcgacc tgtaaatcag agagcagcct tgcggtgacc tgcgaggtct gcgctttggg
     2581 cacacgcaag gtaactcgac catcatcccg gctcaacact tctccatatt gagataactc
     2641 cgtttccgga ttttgtagcg taacgctcag agtcttatag gatgagaatt gattcacgag
     2701 attgcttaga tcgccatcga acaagatgcg gccatgatgg atcacgatca cgcgccggca
     2761 taatgcttcc acatccgcca tgtagtgact cgtcaacaga actgttgcat tattgcggcg
     2821 gttgtattcc gcgataaaag tgcggattcg acgctgcatg gtcacatcta atccaattgt
     2881 tggttcatca aggaaaagga ccttcggctt gtgaagtaac gcggccacga tctccatttt
     2941 catccgttca ccgagtgaaa gattacgaac cggtttggtg accaaatcct ttagctcgag
     3001 caagtcaata aattcatcgc gagtgcgttt gaagtcatcg gcgggcatac cgtagaccgc
     3061 gcgttgcaag tcaaatgaat cgagagcggg caaatcccag gccagttgat tgcggtttcc
     3121 catgaccaat gtgatttgcc gaaggtagtc atacgaccgc cggaagggtt caaacccaag
     3181 gacgcgcacc tcaccagatg ttgggtgcaa gagcccgctc agcattttga gtgtggtggt
     3241 tttcccggcg ccgttaggac ccaggaaccc aaccacctcg ccgggttcga tgttgaacga
     3301 gatctcattc accgccttga cggtgcgata cttgcgcttg accagactgg tgatggcagc
     3361 cttgagtccg ccttctcgtt caggaacctg atagtgtttt gctaactgcg aaattttaat
     3421 aacgggtgcc acaatattct cctcatttga cgaaaggttc agacttcgga agtctttctc
     3481 gaagcacttt cttcacgaag actccaaaaa agacttccga agtctatgtc attccgccca
     3541 tttttaatgc aaaaccgcag ggcgggagcg cgcctgcggc tatctgacgt tctgattgat
     3601 ctacgcaata tcaatcagta gaatcacatg gaggcgcgct gtaagtcaat ataatactga
     3661 atgaattttt catacagacg acctcctctc tacaaagtct ggcagagtgt atcacagcgg
     3721 ccggcgaggt gcaagtgggt tttctggtat aatcttcgct cgtgactggt cctgcctaac
     3781 ggtcctggct gtgggtaagg agacctgcat aaactaaaga aaggaagcat acgtcatgcc
     3841 tttacaaaag gaaatcaaga caaaactgat cactgactac catcgtcacg atgcggatac
     3901 tggctcaccg gaagtgcaaa tcgccattct gaccggccga atccagcagt tgaccgatca
     3961 cctcaggatc aacaagaaag atcaatcctg tcgccgtggt ttgctcaaac tcgttgggca
     4021 gcgccgcaga acgcttacgt acctgcgcaa agtggattac cagcgttacc tggcagtcac
     4081 tgaaagcttg aacctgcgcc acaagtaatt gatttttgta gggacgaccg gttggtcgtc
     4141 cctacctgtc tatcaacccg ctgtcaggaa ttgaacagcg cgaacaacta ggtttgtcca
     4201 cgctcttcag tccctgacct taggcgggaa tttcaggacg caagacggca tcttgcgcca
     4261 tacaggagac aatttaatga aaccagaatc aaagaaatat acaactaccg ttgggaggca
     4321 taccctcacg tttgaaaccg gcaagcttgc tggtcaggca ggtggagctg tcactttcgg
     4381 tacacctgaa tcaatcgttt ttgcagcggc cacaatgggt ggcgtgcgcg agggcatcga
     4441 cttcttccca ctctccgtcg aatatgaaga gcggctttat gctggtggaa gaatcccggg
     4501 ttcctttttc agacgagaag gacgtccggg caccgaagcg atcctcgtcg cgcgtctgac
     4561 cgatcgtccc cttcgtccgc tttttcagga tggcatgcgc aatgaagttc aggtgatcat
     4621 gtacgccctc tcttcagatg gcgtcaaccc tctggatatt cttgccatta acgcggcctc
     4681 tgccgcgatc atgatctcag atattccctg gggaggtcca gtgggtgcgg tccgtgtggg
     4741 gcgcgtgaac ggtgaatttg tcatcaaccc aacgttcgcc gagatcgaaa cctctgacct
     4801 ggacctgagg atcgcgggca ctcgcgaggc catcttaatg gtggaatgtg gcgccaacga
     4861 aatccctgaa gatgtgatgg tacaggcatt ggaattggga cataaatcgc ttcaaccatt
     4921 aatcgactta caattccaaa tgcaagcgga agtgggtaag ccaaaacgag aagttacgct
     4981 gagcctacct gatgctgatc tgcgaaataa agtattcgag cgcgtcaacg gaccgatgac
     5041 cgaactgctc gaaaaacctt tgacgaagct cgagttctat actgggatga ctgagctgaa
     5101 ggaaatggtg gttgccgaaa tgtgttttgt gcctgaaggc gccagcccat cagattatcc
     5161 tgcagtgaat tccgttaggg aggcgttcga gcaggccgaa caagccatcg ttcgcgaacg
     5221 aattctcgat gaaggaaaac gccccgatgg tcgcacccca accgatattc gtccgatttg
     5281 gtgtgaagcc ggtcttttgc caagggcgca cggatccgga ttattcacgc gcggtgagac
     5341 tcaaatcctc tctgttgcta cgcttggtac tttgggcgag gcgcaggaat tggataacct
     5401 cagccctgct aaagacaaac gatatatgca ccactataac ttcccacctt tttctacagg
     5461 tgaggtaaaa aacattcgtg gtcagagccg tcgcgaggtt ggtcacggag ccctggcaga
     5521 gcgcgcgctg gaacctgtca ttcccgcgga ggaatcgttc ccgtacacga ttcgtgttgt
     5581 gtccgaggca ttgtcctcca atggttcaac atctatggga tcggtctgcg gctcaacact
     5641 cgcattgatg gatgctggcg tcccgatcaa ggcaccggtt tccggcgtcg ccatgggact
     5701 gatcacagac gactcaggtc gatataaaat cctgaccgat atccagggca cagaagatca
     5761 tcttggagat atggatttca aggttgctgg caccgccgcc ggtattactg ctttgcaaat
     5821 ggatatcaaa atcagcggct taagcgctca aatgatgaag gaagccctcg aacaggccaa
     5881 tattgcacgc atgtccatcc tggaaaagat gttggcggtt ctgccggagg ctcgcgccga
     5941 gttgaaggag cacgctcccc gaatcatcac tgtgaaaatt ccggtggata agatcggcgc
     6001 gttgatcggt ccgggtggta aaaacatccg ctccttgcag gaagaaaccg gaacgaagat
     6061 tgacatcgaa gaagatggaa cggtttatat cgcctctaca aacgccgtag gcgcaaaaat
     6121 tgcacaggaa cgagttgcag gcctcggtga aaacgcagtg atcggcaaca tctatacggg
     6181 taaggtcgtg cgtattgcag actttggcgc gtttgtggaa attctgcccg gcgtggatgg
     6241 gcttgtgcat atttcacagt tggatagtga gcgcgtgaac agagttgaag acgtcgtcgg
     6301 catgggtgat gagattaccg tcatggtgac ggatattgac ccgcaaggga aaatccgact
     6361 ctcgcgacag gccgtactcg aaggctggac tgctgaagag gcccgcgaga aggatggtcc
     6421 gcgcagaagc ggcggtggta atcggggtgg cgaccgtgga ggtgggcgcg gcggtgatcg
     6481 cggcggcgga cgaggtaacg atcgcggtgg tttccgtggt ggtaatcgtt ctggtggcga
     6541 ccagaatcgc ggcggacgca attaattttc gataaaccct tgtcagggca tggcaaacaa
     6601 ccatgccctg atttttattc aagtgatttt cactttttaa aggtaaattc atattgcgcg
     6661 cccgcaaagt aaacaatcta ttaacagtca tatccttacg tttaaagatg ggtaattaac
     6721 tctgaactgg atgacggttt aagtgtgaag ttttgctgac gcttattaga ggaaaccatt
     6781 taagatatct atatcagacg aattccgttt catcatgcag gtagccaatt aattcagctt
     6841 caatacttca ataatcttgg aaatggagaa tatactcaat gaaggctgta cgaattcacg
     6901 aatatggtgg gcacgatgtt cttgtttatg aagaggcccc gaaaccctct cctcgggtag
     6961 gagaagtcct tattaaggtt catgcctcag gtgtgaacct ctttgattgt gcagtgcatg
     7021 caggctatat gcatcctttc ttcaactata catttccatt ggtcatcgga acagatgtat
     7081 ctggcgtggt tgaagaggtg ggagaaggtg taacagatgt ttcgcctgga gacgccgttt
     7141 atacaagagc tggagtgttt caggatggtg ggaacgctga atatgttgta gcgcccgctg
     7201 caaatgtcgc acccaaacct caaaccattg accatatcca cacagccgcg attcctcatg
     7261 ttggttttgc tgcatggtat gcacttgtcg atttagctca aattagcgaa ggtcaaagtg
     7321 tcttgattca cggagccgcc gggggtgtag ggcatattgc agttcaactg gcaaaattgc
     7381 gtggcgcgaa agtgattgga actgcctcgg tcaatttaga ttttctgcat gaacttggcg
     7441 tggatgaggc cattaattat tctacaactt cctttgaaga cgtcgtgaag gatgtagatg
     7501 ttgtactcga tctgatcggt ggtgaaactc aggaacgctc atggaaatta ataaaaccgg
     7561 gtggtatctt ggtcacaacc caaacttatc cgccacccga agaagtggca atagctcatg
     7621 gtgtgcgact tggacacgtc aatcaaaatc caccgatgag aatggtactg accgagtatg
     7681 cgaagatgat cgatgctggg cagctcaaac cacacgtttc cacagtgttg ccgctggcag
     7741 aggtccaaaa aggacatgaa ttaatttcaa cgatgcatac acgtggaaaa attgttttgc
     7801 aagtggtgga ttaagccctt cgcaaacccg aagaataatt cgctaaaacc agccttttca
     7861 tgaaacggaa aggctggttt cttaatttaa tggttctacg aaccatattt gacgaaaaag
     7921 gagcctattc ctcatacgat gccacaatga ccacagattt ttgttatcaa aattgaacgt
     7981 ttttaaggat ttctctagat gtataatttg caaatccggt caaattcatc ctgactatga
     8041 gagacgggct tagatgaaaa gatttttcgt ttttttggta ccttttgcca ctctattttt
     8101 tgtccttttg gcaattacag ccactgcgaa tgcaagtggc aattttcaga ctgatgtact
     8161 tgaacaaggt aaatacattg ccacgattgg tggatgtatt gactgtcata cgccattaga
     8221 ggatgaattc caagacccca cgcaaatgac gcctgagcag ctaaaagtct tgtcattcaa
     8281 tctgggtgcc gctctcgacc aggaaaacaa gctccttgcc ggaggtaggt tttttgacct
     8341 tggtccggct ggtgttctcc tgacacgcaa cctaacagct gaccaggaaa gtggtctggg
     8401 atcctggact gatgaacaga tcaaactcgc cataaaaacg ggtatcaata acgaaggcac
     8461 tttgcttttt ccggtaatgc cttaccacgt ttacaacacg atggcagatt ccgatctgga
     8521 tgcactcgtt gcctatcttc ggaccgtcag agcgatagac aattccattc ctaaaaatac
     8581 aatttcgaca cagggctttc ccataccctc ttatgaaaca ggcattatcg cccccgatcc
     8641 atccgataag gctgcgcgcg gtgcctatct cgttaatagt gtaatggcat gtactgattg
     8701 tcacacacca gttgacccgg caacgggagc cccacaaatg gaaaagtatc tggcaggtcg
     8761 tcagccatat gaaggtccgt ggggcatcgt ttatggcggt aacataactc cggacaatga
     8821 aactggcatt ggcagttgga cggaggacga aatcatacgt gcaatcacaa cgggggttgg
     8881 gaaagatggt cgccgcctga tcctcatgcc ctggtttaat tacgtcgcgc ttaagccaga
     8941 ggatgcgcag gcgatcgcct actacctcaa gaacgtctta cccgccgttg acaatgaaat
     9001 tcctgcggct tctctaaaca aagggttcga agaaatagtc cccggagtcc agaatcagtc
     9061 aacagccgga gaattgatgt catctcccgt tatcctggta atcgttggaa ttggcgtcat
     9121 cctggctgtc tcattgattg catcctcttc caaaagaaat cagcctaaat aatgacacaa
     9181 gccattaacc ggctcctcaa agaaaaatat ctcaatattc aggccaaact ggtatttcca
     9241 ttggccatca caattatcct gcttgtcgtc gttctttccc cattcacaaa tcgtctgatt
     9301 acaaaccgaa tcgaagacga agcagatcgc aggctaagcg agatcgcagg ctctgttggc
     9361 gcgctgatcg aaaactctga agcactggct cgcaataacg cagccctgct ggcagaacag
     9421 cctgatgtgg taaatgcatt ttctgattta agttcttcca cacagtcact gaatgcgagc
     9481 aaagtgtctt tagatctgca ggagttaatt ctttataaag cggactttaa acccggggat
     9541 caagcctatt tttatggagg gcccgccgtt gctcgccgtc tacaggtaag cgaggatatc
     9601 aacgacattc gcgatgaact tattctcagc gctctggaaa gtaaagcact aaacagcagc
     9661 gtggccatcg ctccgcaaag ctcccagatc atcggtgtgg caccggtttt tgcggcagag
     9721 ggaggagatt tgttgggcgt ggttgtcgca acgttctaca tggatcaaag ttatattgat
     9781 ggaatcagcc agatcatcaa tacagatgta gccattgtga aagataactc tgttatcgtt
     9841 tctacaattg atcagaatgc gggcattgag cgcctgatca atcagggctg gcttaattct
     9901 tctgaaacac cttcccaaaa cgtaacttat agcaccaatg aggatttccg tttgttgggc
     9961 cacccattgg taatttcagg gaatcagcaa ggctcggtga tagtagcgca accaattgat
    10021 aatctttttg ccgtgagtcg aaatatccag ctgattctcg gtactgtcac aggcatcttt
    10081 ggcatcactg ctctctggtt ctggatcgca gcatttttgg cgtttacgcg tccgcttgca
    10141 caattgacac aggcgaccac taatatgagc cagggcgact taaatcagaa agttaaccca
    10201 tcttacgtgc tgtttaaaga tgaaattacg atcctaagtg agaatttcaa tgcgatggct
    10261 gcaaacttga gcgactcgta tgcaagcctg gaagaaaaag tggagcagcg cacgcatgag
    10321 ttggccgatg caagagatga agctgtggcc gcgaataaat ccaagtctga atttatttcg
    10381 gtcgtatcgc atgaactgaa actccccatg acatccatca aaggctatag cgatctgatg
    10441 ctcgcaggcg cgaccggacc cttgaatgaa aaccagacca gtttcctgac gacgatccgc
    10501 aataacgtca atcgcatggc cacgctcgtt tcagacctgg cagatatttc acgcattgag
    10561 agtggtaacc tgcgccttga agcacgttcg gtcccggttt gggatgttat cgacgaagtc
    10621 gttacgctga ccaagacaca ggtaacagaa aagaatcaag tcgtcacagt ggatatccca
    10681 acggaactcc cgaaagcctg gtgcgaccgt aaccgactgg cgcaagtcct taccaatctg
    10741 gttagcaatg ccaacaaata tacacctgag ggtggtgcga ttgtcgttca ggctatccgc
    10801 aagaatggca tgatccaggt caaagttgag gataacggtc atggtatgac cctggaagac
    10861 caggcaaaac tatttaccaa gttcttccga tctgccgatg acaaaatccg cgaagcccct
    10921 ggaaccggac ttggattgag cattacgaag aatcttatcg aactgcaagg cggaaaagtt
    10981 tggtttgaaa gcgagttccg caaaggaacg tcgttctatt tcacggtgcc gatcagcttg
    11041 gatgggaaca cgccaccgcc ctagtatcct gtatctgagt atgcgtaacc gagagccata
    11101 tcgcccttga tatggctttt tgtttttcaa cagctgaagc attctcacaa cttgggtgaa
    11161 aagaataccg aaaaacatcc actcctctcg tttattaaga tagctaacat gattatccaa
    11221 gtagaaagga tctactacct ttaagaaaac ccctaggagg gaaaataaat gctaccaggt
    11281 cttaccgctc gtgaacaaca gatactcgat ttgatcgctg aaggattgac caacagacag
    11341 atatcttcga atctctacat cagcgaatct acggtagaga atcatatcca tcatattttc
    11401 gtaaagctag ggatttccaa cagggcacag gccgttgctc atgctttcca gttgaagatc
    11461 cgtttgccaa ctgacatatt cgaaaatgaa gggaatcctt cttgataggc acttaataat
    11521 atgatagtaa aatcagccat tacattagac cgcgacgatc tcctctatca tgttaatcat
    11581 cgactcccac ctcgacctgg catggaacgc cctacaatgg aatcggaacc tgcaacagtc
    11641 cgtgtatacg attcggacac aggagggcaa tctgacaggt ccggggcgag gtcaggggac
    11701 ggtcgcgtta cctgaattac gcaacggacg cgttgcagtc tgtttcgcca cgctgctggc
    11761 gcgctccact ggaaaaattg aaccagggtt ggattatcaa tctcctttac aggcctatgc
    11821 tgcagcacag ggacaacttt cttattatca ggcactgact gaatcggggg aagtgagact
    11881 gataaaaaac ctctcagact tagacaatca catgctcgag tggggacagt gggaaaacga
    11941 tcctcagagc acgcatccac caattggctt gatcatcagc atggaaagtg cagatccgat
    12001 cctgaaaccg gatcaattat cggcgtggaa agaggctggt gtgcgaatca tcggtcccgc
    12061 gcactacggt ccgggacgct atgcaggtgg gacatctacg gagtctggtc taactcctga
    12121 tggattcgcc ttgctcagtg aaatggatcg gctcgagatc atccttgacc tcactcattt
    12181 atctgatcag gcattttggc aggcgctgga tcatttcggt ggaattgtca tcgccagcca
    12241 caacaactgc cgttcgctgg ttcctcatca acgtcaattc gacgatgaac agattaaagc
    12301 gattcttgct cgccagggcg tgatcggggc tgcacttgac aattggatgc tgactccaaa
    12361 ttgggtgcgc ggcgctcgcg acaatccacc ggtcaccctt gagaatgttg cagatcatat
    12421 tgactatatc tgccaactcg cggggaatgc agaacacgcc gctattggca gtgacctgga
    12481 tggaggcttt ggccgtgaac aatctccgac tgacctggat acgatcgcag atcttcaaaa
    12541 gttcgtggaa attttgactg gacgtgggta cagaaacgaa gatatttcac tcatcatgca
    12601 tggaaactgg ttgcggcttt tgcgtggcgc ttgggtaggc taaaatatat ggttgcgaca
    12661 acctgctggt cgagtagctc tgagtgttct tcgcgaagag cgtatcgaga ccacaagtaa
    12721 gaagataatt tgttttcaag ggtactttgg agaccgactg gtttcgatac gtcccgcgaa
    12781 aagcctgcgg gactactcaa ccagcggagt atttttgaaa ctaaaggaga atcatcatgc
    12841 ccaagcaaca aatcaccacc aatactggcg cgtcccctat gggagcctat tcacagggcc
    12901 tgcgcgcggg agatttcatc tttgtctccg gtcagggtcc gattgacccg gatacaggaa
    12961 aaattgtcgg tgacaccatt gaagagcaga cggcgcgtac cttggataac ctcaaagcta
    13021 ttttggaggc gggcggagcg acgatggcag atgtggtgaa ggtcacagca cacctgaccg
    13081 atcttgcaaa tttcgcagcg tataacaaag tttatgcaac atactttccg gatccgaaac
    13141 cagttcgcac cactgtcggg agtcaactcc ttggtatgct ggttgaaatt gatgccatcg
    13201 cgtatgtcgg agagaaatag caaatgcgcg ttgacgaatt ggaaacacct gttcccgttg
    13261 tggacatgga ccgccttcaa tctaacattt cccgactgca gtcctatctt gatgaacagg
    13321 gaattgccaa ccgaccacat ataaaaaccc ataaaatccc cgccattgct cagatgcaga
    13381 tggacgcggg tgccattggg attacctgcc aaaaagtcag cgaagccgaa gtgatggcgg
    13441 acgccggatt cacagatatc tttattccgt ataacatcat cggcgaaagc aagttgcagc
    13501 ggctgatgtc gcttgcaagc agaatcacag tgagtgtcac tgcagattct gcttatgtaa
    13561 tccgtgggct ttcagaagca gcacaacagg caggactgac actgcctgtt ctgatcgaat
    13621 gcgacaccgg tgcacatcgt tgtggcgttc aatcaccgca ggaagcagca gacttggcgc
    13681 gcctgatcaa tcaatcgcca aatttgcatt tcggtggatt gatgacctac ccgaccagcg
    13741 aggagacaga taaatttgtg cgcgaaacgc gccagcttct ggcagaggat aacttgcaga
    13801 ttgaacgcgt gagtggtggt ggaactccca cgatgtggca ggttcatacg cttcgcgaag
    13861 tgactgaaca tcgcgcgggg atctatgtct atggggatcg tcttaccctt cgttcgggcg
    13921 cagtgacgct ggacacctgt gcgttgaaag tgcacactac gattgtgagt cgtcctacag
    13981 ccgaaagagg tatcctggac gctgggagca aatcactttc aagtgatttg catgggctgg
    14041 atggatacgg ctacatctgc gaataccctt acgcaaaaat ttatgctctg tctgaagagc
    14101 acggtcacgt agatttctca gcctgtgaac gcaagccgga aatcggtgaa cgggtgacgg
    14161 tcatcccgaa tcactgctgc acggtcacca gtcaatttga tgaagttgtc ggcgcgcgca
    14221 acggtgaagt ggaagtaatt tggcaggtca cagcccgtgg aaccgtgcga taaatgagcg
    14281 caatcgaaac cgccagcgag gcgatcaagc aaagtggaat cattgccatc ctgcgcggtg
    14341 atttctcggt ggaagatatg cttcgaatcg gcaaagcgtt gctgcagggc ggagtcactg
    14401 tgatggaagt cacactgaat tctccggctg cgctcgaagc attaccaaag ttgcgaaagc
    14461 aatttgaaga tgagatgctg gtgggtgcag gaacagttcg gaatgcggat catgcaaaat
    14521 cggcatggga ttcgggggca caattccttg tctcgccgaa ccttgatgtg gatacggttt
    14581 cctttgcacg gacgaaagac ctgctccacc ttcccggagt ctttaccgcc accgaagcac
    14641 agaccgcttt cgctgccggg tgccgaatgc tcaagctgtt tccaatggaa gctttcgcaa
    14701 acggaccagc gtttctcaag gctttgcgtg ctccgctcaa tgatattagt tttgtgccga
    14761 ccggcggtat atcccttgaa aatattgcgg actatgctcg agcgggtgca gtcgcagttg
    14821 ggttgggaag caaactggtg ttgagtcgcg aacaatcgtc gaacgatctt gcagctcgcg
    14881 cgaaagcctt acggcaaacc tgggaacaag ccaggtatgc ctgagccgcg attcgacgta
    14941 acaagcattg gagagatgct gctgcgctta agcgttccat cgggcgaacg gcttgagatg
    15001 gcgcggcaac tggatgttta ccccgcggga gccgaagcca acgtggtgag cctgctggct
    15061 cgtctcgaac ggaaaaccta ctgggcaggt gccttgccag agaattctcc cgggcagctc
    15121 gcggcgaatg cgctacgact ttcaggggtg gatacgagcg gaattatctg gaacgaggcc
    15181 ggacgaattg gcacgtatta cgtcgagttt ggcgaaccac cacgcgggat ccaagtcacg
    15241 tatgaccggg cggattcgtg catgtctcat ctgaaggcgg atgaaattga ttgggatgct
    15301 ttgttagata cgcgcttact gcatctgaca ggaattacac ccgcgctttc gtcttcctgt
    15361 cgcgagattg tggaacgggc actagatgaa gcaagacagc gcagtgttcc agtcagcttt
    15421 gacgtcaact atcgaggaaa actctggact aaaacagatg cgcacaatat tcttagtcca
    15481 atgattcagc aggttgaatt gttattttgt agtgccagag atgcaatagc tctgttcaat
    15541 tgcacaggca cctttgatga aattgcacag aaattgctcg aactgagtca agcaaaattt
    15601 gtggcggtta caaacagtaa ccagggtgcg cttttatggg atggaaaaga atggctgcat
    15661 gaaccagctc gctcaacgaa aatcattgat cgtttgggag caggcgatgc gttggccggg
    15721 ggtgtgatcc atggttggct ggatggagat ttaagagttg gcttgcgtta tggcgtcacg
    15781 ctggcggcac ttgcgctaag tcaatttggg gatatggtaa tcacaaacaa ggacgagtta
    15841 cttgcactga gtcagggtga ttccactctc acgcgttagt ctttcgtcgt aggtcagata
    15901 gacaacaccg catctgcggt tcggtgcagg tgtccgaact acatctcaca catccaaaat
    15961 cagctgcgcc gtatggggca gtattcatcc catagtaaaa tgaaggcatg gaagttcaaa
    16021 tcgcggttgc gaaagtgaac aaatatgcca catcggaaag cggggataca ctagaagtcg
    16081 tcgagcgtcc aaatggcggg ctgtctgttg tgctggcaga cgggcagact tccggacgag
    16141 gtgcgaaggc cgtatcgcaa ttggtcgtgc gaaaggtgat tggtctgctg gcggaggggg
    16201 tccgtgacgg agctgcggcg agagctgcat cagatgcctt atacaccgat aaacaaggca
    16261 aagtcatctc cacactaaac attgcatccg tcgatttcta tagcgggacg atcgtattga
    16321 cccgcaataa tccatcgccg atgtttattt gtcgtggcga tcaaattgat tgtcgtgatg
    16381 aagagagcat tccgttgggc acgtcacgta acgttcggcc cttgatcacc gagatcaaca
    16441 ttgagccgag gctgattatt gtcatttaca cagatggtct cactcatgcc ggcaaacgcc
    16501 gcggccagcc tatggatgtg cgtatcactc tgcgctccat cctcgatgga caggatttgt
    16561 caccacagac gattgccgac tcgttgttgg ctgaggcggt cagcctggat gacaaccgcc
    16621 cggccgatga catcagcgtg gtggtcctca aggtcacggc acgttctgga gataatgtgc
    16681 gccgtatgac ggtgcgcctg ccaattgagg ggaattccag gttatgaaat tagtgtggcg
    16741 acaatggaag gcagcctggc gcgacaccct gattctgatc aacgaatttc gcaccccatt
    16801 gatcgtcttc accctgatag tgatcggtgg aggcttatac taccgtgcac tggcaggtcg
    16861 gctcggggaa cccattcaga gcgtagggga agccatcttt atcatcctga gtgccgcgtt
    16921 tttgcagatc gtcggtgaat tcccgcagca tccgtattta caattatttc actttttcat
    16981 gcctttgatc ggcgccatcg tccttgcaca aggacttgct gacttcggca gtttactttt
    17041 caatcgccac acgcgaagca aggagtggga aatggctgtt gcctccacac tgaataacca
    17101 catcatcctc gttggactgg ggcatctagg gtatcgtatc gtccaaaaat tgaatgagat
    17161 gggcagagaa gtagctgtga tcgagctcaa tccgtctgtt gactcgttca aagctgtgca
    17221 aaaaatgggg gtgccagtta ttcaagatga tggtacacgt gtcgcaacat tggatgcggc
    17281 aaacatcaag gatgcacgca caatcatcct tgccgggcaa aacgacgcga tgaacctgca
    17341 gatcgccctc aaagcacgca gtctaaaccc gaatattcaa gtcgtgatcc ggatctttga
    17401 tgtcgatttc gcgcatgcgt tacatgatca atttggtttt gtggcgttaa gcgccaccga
    17461 gatggctgca cccgtcttcg ccgccgcggc agcgggcgca gacgtaacca accccatctc
    17521 catcgaaggt cagcagctga gcctggcacg catgacaatt tctccctcct ccagactggc
    17581 aaataaaaca gtgggttatg tggaagacaa ttaccggctc aatatcatcc tcctacgaca
    17641 cggtcaggac tctgaaatgc accccaccga tacgcgttca cttcaagcag gggatacact
    17701 cgccgtgctg ggtggtccgg aacagcttag gcaactgatg caagacacca gataatggag
    17761 cctccaagta gcacacgact gattggtatt gtaggtccgt gcggatcggg caaatccgcg
    17821 ctcacagagg ggctcgaaaa aaaaggatat acctgtcggc atatcgccca ggaacattcc
    17881 tacgttcccg atatgtggca ggtgatgacg aatccaaata ttttgatcta tctagatgta
    17941 tccttccaga caagtactga gcgaacaaat ctaaattggc aggaaaagga tttcctagaa
    18001 caactccgca ggctctcgca tgcccgagcg cacgcccata taatcattga aaccgatact
    18061 ctgacgattg aagatgtttt acaaaaatcg ttagattacc taaaaagacc ctaaaggatt
    18121 ctatcgttgt cttttagaat attaaaagca gagcacttgt atctaccttt taccttatta
    18181 agccgagaaa tcctttaggg tctaatgaaa aggattcttt aacttgagaa aacttctcat
    18241 atccctcctg accttcagct tgttcctgct ttcctgttcg atgccccagc ccagtgctac
    18301 gccgccatcc gtcgaaagaa cgccggatcc attacaaacg ccctgggagg atcgttccat
    18361 cttcaaggag ggacttgtca aatctcaaca atccgttctt gatgaattag atggcgcaag
    18421 tatttaccat attgaattta atatcagcga cgatttgctg catattgcag ggactcagga
    18481 cgttcggtac acaaataatg aagacgtcgc attcgatgct gtgtatttcc attcctttcc
    18541 tactatcctg ggtgggcaga tggttatttc atccacaagg ataaatgata cggaagctga
    18601 tttttcattt gagttccaga atagcgtctt ccgagtgccg ctgcagtcag agttactgcc
    18661 tggtcaaagc acaatcatac atatcgattt tgccgttacc gttccccaaa ctctcgaatc
    18721 aaactatggc atgttggctt atgcggaaga cgtccttgcc ctggcacatg cctatccatt
    18781 gattgctgtt tacgatgatg aaggctggaa tatagagccg ccatctcaaa acggtgatgt
    18841 gacctatgcc gatgccgctt tctatatcgt ccgcatcaac gcgccaaagg agttgacgct
    18901 ggtgacttcc gggcatcaga taagtttgcg cgaggacgat cagcttcaat cgctagtggt
    18961 tgccagcggc ccggcgcgcg attttttcct ggcagcgagt ccaaagtatg aggaaatttc
    19021 gcaaaccttt ggagagatca ctgtccgtag ttatgcgcca aaggaatctc gtgacggctc
    19081 gcaactggcg ttggatgtgg ccgcccgcgc catcgaagat tttagcaagc gttatgcacc
    19141 ctacccctat actgaatttg acatcgcatc cactccgacc ctggcgcttg gcatcgaata
    19201 tccgggactg gttgtcataa cctcagagat ttacgatttg aatcgtaaca ttcgtggcgc
    19261 tccagccagg gtttacctgg agtcgactgt cgcccacgaa gtgggacacc aatggttcta
    19321 taatctggtc ggggatgatc aacttgacga tccctggatt gacgaatccc tgacgcaatt
    19381 tgtcacactt caatattata tggatgcata cggcaccgcc ggcgccaatg gatttcgtgc
    19441 ctccttggaa gaacgatggg cacgcgtaga taaggcggat atccccattg gtttgtcagt
    19501 ggccagttac gaaggcccgg agtatggagc gatcgtctac ggacgcggtc cactattttt
    19561 tgtcgcatta aaagatgtaa tgggtgcaga gaattttgac aatttcttaa aggcttacac
    19621 agaaaccttt tcatggaagg tcgctacacc cgaagggctg caatctctgg cagaaaagca
    19681 gtgtgcgtgt gatctcgatt caattttcaa tgaatgggtt tatccgtagg gaaaacacct
    19741 gaactcccga tttatcctac acacgcccat tcaatgggag tataatcacc gccgcgccag
    19801 ccataggctg gcattttcat ggatggagat tatcacggca tgattcgaaa cctgtataca
    19861 cgtctggctc tgattgcggt catcgctgcc ctggctattt gggtggatct gacagacgtt
    19921 gtccgactac gaaatcccct gaacgatgac attgtagctg aacgaaatat tacgccccgt
    19981 ctggggctgg acttgcgggg tggcttgcaa gtgctactcg aagcggacct gccagcagat
    20041 actccggtag atgcagaagc tatggaaact gctcgcagca ttgtcgaaaa ccgtaccaat
    20101 gctttgggcg tcagcgagaa cctgattcaa ttggcaggtg accgccgcat tgtaggagaa
    20161 tttcctggtg cggaagatgc ggatgccatt ctcgatatca tccaacagac cgggttgctt
    20221 gaatttgtag acacgggcga tttcagacca gaagaaggaa ccgtccttca aaccgacttt
    20281 gcaacgggtt cttcaacgga acctgcggtc actccaactc ccacaacgga tggaacaacg
    20341 ccgccaacgg tctatcacac gatcatgact ggaaaagaac tcgaaagcgt taatgtcagc
    20401 gcaggtcaag taggcggcgc cgtgatcgac ttcacccttt caccagaggg aaccaccatc
    20461 tttgggcaac at
//
LOCUS       DASTJP010000248         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_882276, whole genome shotgun sequence.
ACCESSION   DASTJP010000248 DASTJP010000000
VERSION     DASTJP010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882276"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFQ23_07620"
     CDS             <1..377
                     /locus_tag="VFQ23_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906492.1"
                     /translation="IYGTEWIRAFLGGSLSALVVGIHLLLFTLVTLKEIALPGIIFVL
                     YLALDLPGILLHTWLFSDPFATLLPTAEAEAIGWRIIWLALVFWFFSGFALTYFIKND
                     RKAVIVWFLTIVILAGTAKIWS"
     gene            complement(427..921)
                     /gene="folK"
                     /locus_tag="VFQ23_07625"
     CDS             complement(427..921)
                     /gene="folK"
                     /locus_tag="VFQ23_07625"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET9906493.1"
                     /translation="MNEFHNAYLNLGSNIQPEMNIARGIDLLKKYGEIRKISTVWESE
                     SVGGPGPNYLNVCLLFVSTYTKAQLKEQVIDIIETQLGRIRTEDKYIPRTIDIDIILF
                     DDKHTNAKDWESAFMIVPLAEIYPEYQNPFTGEIIGATAERLRKLTWIQPRPEVIRGN
                     DPVS"
     gene            complement(914..1270)
                     /gene="folB"
                     /locus_tag="VFQ23_07630"
     CDS             complement(914..1270)
                     /gene="folB"
                     /locus_tag="VFQ23_07630"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET9906494.1"
                     /translation="MDKVIIKDLLARGIIGVNDWERKRPQDILINITIFTDTRRAAES
                     DNINDCVNYSTISKKIIAHAESSQRLTVEALANDLAKLCLEEDSAQKVIVRVEKPGAV
                     RFARSVGVEIERSRDE"
     gene            complement(1358..2086)
                     /locus_tag="VFQ23_07635"
     CDS             complement(1358..2086)
                     /locus_tag="VFQ23_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9906495.1"
                     /translation="MEDLPLKNKSILVTGAAKRVGRIFALACARAGADVVIHHGHSDA
                     EAEKVCDEIKELGGKAWVFKAELTDSSVVQGLIQRVNESTPLYGLVNSAAIFEPLTLE
                     QTTIKAWETHLAINLTAPFLLSQAFAQQAKEGGRIVNILDWRALRPGADHFPYTISKA
                     SLAAMTKSLAVALAPTITVNGLALGAILPPSDGARNPDIIKNVPMKRWAQEGEIQQAL
                     IFLLTSPDYITGEIIHVDGGRHLV"
BASE COUNT          537 a          550 c          514 g          677 t
ORIGIN      
        1 aaatctacgg aacggaatgg attcgagcgt ttcttggtgg ctcactatca gctttagttg
       61 ttggtatcca cttgctcttg ttcacgcttg tcacacttaa agaaatagca ttacctggaa
      121 taatattcgt tctctacctt gcacttgatt tgccaggtat tcttcttcac acatggttat
      181 ttagcgatcc attcgcaacg ctattaccta cagcagaggc agaggctatt ggttggcgaa
      241 tcatatggct agctttggtg ttttggtttt tctccgggtt cgcattaacc tacttcatca
      301 agaacgatag gaaagcagtt atcgtttggt tcctcacgat agttattttg gcaggtacag
      361 ccaaaatttg gtcgtaaagg taggaatttc ttgataatat ctcgagaggt cacagccact
      421 gcaaatctaa cttacgggat cgttccctct tattacttcc ggccgtggct gtatccaagt
      481 cagcttgcgg agtcgctcag cggtcgcccc aatgatttcg ccggtgaagg ggttttgata
      541 ctcgggatat atttctgcca gcgggacaat cataaatgca gactcccaat ccttggcatt
      601 ggtgtgttta tcatcaaaca gtatgatgtc aatatctatc gtgcggggga tgtatttatc
      661 ttccgttctt atccgaccca gttgagtctc gatgatatcg ataacctgct cctttaattg
      721 cgcttttgta taagtagata caaataacaa acatacattt aaatagtttg gtccgggccc
      781 cccgacagac tctgactccc atactgtaga aatttttcga atttccccat atttttttag
      841 aagatcaatg ccccgtgcga tgttcatctc cggttgaata ttcgatccaa gatttagata
      901 agcgttatgg aactcattca tcacggcttc gttcgatctc gacgcctaca gaccgtgcaa
      961 agcgaactgc tccaggtttt tccacacgga cgattacttt ttgtgcgcta tcttcctcga
     1021 gacagagttt cgccaggtca tttgcgagcg cctcaaccgt cagccgctgt gacgattcgg
     1081 catgggctat aattttcttc gaaatcgtgc tgtagttgac gcagtcatta atgttatctg
     1141 actcggcggc gcgcctcgta tccgtgaata tggtgatgtt aatgagaata tcctgcggac
     1201 gttttcgctc ccagtcatta acgccgataa tacctcgagc gagtaaatct ttgataatta
     1261 ccttatccat cgtgaattag ttccttatga tcatgcttgc gtgtaggcac aatgattcct
     1321 cttttctgga aaaatcgttc actcaaaaac tcagcttcta aaccaaatgc cttccgccgt
     1381 caacgtgaat gatctctccc gtaatatagt cagggctggt caacaggaaa atcagcgctt
     1441 gttgtatctc tccttcctgc gcccaccttt tcataggaac attttttatg atatccgggt
     1501 tgcgtgcccc atcagagggc gggaggatgg ctcccagggc taatccattg actgtgatgg
     1561 tcggcgcgag cgcaaccgcc agggattttg tcattgccgc cagagacgcc ttgctgatcg
     1621 tgtacggaaa atgatccgca cccggcctaa gcgcccgcca gtccaaaatg ttgacgatcc
     1681 gtccgccttc tttcgcctgc tgggcgaatg cctggctgag caaaaacggc gcggtcaaat
     1741 tgatcgcaag atgtgtttcc caggccttta ttgtggtctg ttccaacgtc agcggttcga
     1801 aaattgcagc gctgtttacc agaccgtata agggtgttga ctcgttcact cgctgaatca
     1861 agccctgcac tacggacgag tcggttaatt ccgctttgaa aacccaggcc ttgcctccga
     1921 gttcttttat ttcatcacaa actttttcgg cctcggcgtc tgaatgcccg tggtggatca
     1981 caacatctgc gcccgctcga gcgcaagcca gagcaaatat ccttccgacg cgtttcgcgg
     2041 ctcccgtgac caggatcgac ttgtttttta gagggaggtc ttccatttcg aaaaattgta
     2101 tcattgaaca tgtgtaccta tcagtgattt ccacgtcatg tcattgacgc aagaaatcgg
     2161 ttgtgctaat atacttttgt ttcgaccaag gagaaaccca tgcgtgttct aacagacaga
     2221 gtatttgttg aagaccctat tgagtttagc cgttggtaag gtgaaaaatg gataagaa
//
LOCUS       DASTJP010000249         4531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1289644, whole genome shotgun sequence.
ACCESSION   DASTJP010000249 DASTJP010000000
VERSION     DASTJP010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289644"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFQ23_07640"
     CDS             <1..998
                     /locus_tag="VFQ23_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9906496.1"
                     /translation="MKDVKRWLPGAVISILLIAAILYFVDLRAMVNAIRNANFGLLAI
                     AIAVGFVWMWVRSIVWWTLLRKRATLKDVFFTVGEGYLLNNFLPFRLGEIGRAFLLSR
                     KSDLQFMEILPTIVIERVVDLGFSAIVLLAALPFVVGAEGAGQIGITVGIIVFIGIVM
                     LYVLARYNQWALDIFHKLSARWPSLQKFGGSFLESFFAGLGVLTDGWLFLRFLFWMTL
                     NWGIAIFSYYLIILAFFPEAQPVWGMFGLGAAAFGGAIPSLPGAVGTFEGAFGGALTL
                     LTGDESTSLAVALTGRLYNYINSAVIGGIGLVREGQTLSGIYEQIRNFRTRSNQA"
     gene            995..1660
                     /locus_tag="VFQ23_07645"
     CDS             995..1660
                     /locus_tag="VFQ23_07645"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9906497.1"
                     /translation="MIPLYDTIRPRQFPFINWLLILFNAVVFWYELQLGESALQDFIF
                     TWGLVPAELLSNPSEEWQTIFTSMFLHGGWFHVISNMWVLYIFGDNIEDRVGSIRYLI
                     FYLLCGIAAAALQIFVMQSSAVPMIGASGAIAGVLGAYLISFPRSRIASLVPILFIFT
                     IMEVPAFLFLIFWFVSQLYSGLFAMQGGGASGIAWWAHIGGFVFGLIAVSFFRRRIMY
                     RGY"
     gene            1803..2828
                     /locus_tag="VFQ23_07650"
     CDS             1803..2828
                     /locus_tag="VFQ23_07650"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9906498.1"
                     /translation="MARNYFVTGGAGFIGSNYVHRLVERGEQVTIFDNLSRGGAPRNI
                     AWLQETFGNDAFKLIVGDLRDADLIQESAKDADVIVHLAGQVAVTTSVNNPRDDFEAN
                     ALGTFNALEAARLSDRNPVFIYSSTNKVYGGMEDVEIIEDATRWLYKDLEYGCSEAQP
                     LDFHSPYGVSKGAGDQYTRDYSRIYGLRSVVFRQSCIYGPRQFGVEDQGWLAWMMIAA
                     VTGKQITVYGDGKQVRDVLHVNDLLNAYDLAVEKIDDVKGEVYNVGGGTRNVMAIWAE
                     FGPILERLIGRKIEVARDDWRPGDQRVFYADFRKAQRELGWEPKIDLEEGMELLFDWI
                     NHNRELF"
     gene            2987..4519
                     /locus_tag="VFQ23_07655"
     CDS             2987..4519
                     /locus_tag="VFQ23_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9906499.1"
                     /translation="MNLRLNRRDFLKLAGLVPLSLSATRLLQSLDGTQRLQGTQKNVI
                     VIVFDAFSAYDISLYGFPRETTPNINRLAKRAVVYHNHFAGGNFTTPGAGSLLTGVLP
                     WTHRAFNHNQKVATPFIDNNIFSAFKDYYRVAYTHNGFANTLLKQFHEYIDEFIPREK
                     YFLESIDTLISALFKNDDDIASISWLRSMRIEENGGFAYSLFLSHIYEAIQQKRIEDL
                     KKDFPRGIPTTGADYGYLLETAIDAVGNRLSEIPQPFFGYFHFMPPHHPYRPPREYVN
                     AFKGDGFKDVEKPVDILVRQEVKNLPMKRTEYDEFILYCDREFGRLFDQLESSGLLEN
                     TWLVLTSDHGEMFERGFAGHGSKLLHQPVVRIPLMIFEPGRDSGMDIHEYTSAVDVLP
                     TLTHVTGINTPGWGEGIVLPPYATTSTGSNRNIYVVQAIDNSPTAPLTEASTTLIKEN
                     YKLHYYFGYPEAGNAEIVKLFDIQADPDELIDLASSKSGIASELLDELQRKLKEVNEP
                     YL"
BASE COUNT         1080 a         1044 c         1173 g         1234 t
ORIGIN      
        1 agatgaaaga tgtaaaacgc tggctgccgg gcgcggtcat cagtatcctg ctgatcgctg
       61 ccatccttta ttttgtcgat ctgcgcgcca tggtgaatgc gatccgcaac gcaaacttcg
      121 gcttgcttgc tatcgcgatc gccgttggtt tcgtgtggat gtgggtgcgc tccatcgtgt
      181 ggtggacgtt attacgaaag cgagccaccc ttaaggatgt gttctttacc gttggagaag
      241 gatatcttct aaacaacttc ttaccgttca gactcgggga gatcggacgt gcctttctac
      301 tcagccgcaa gtcagacctg caatttatgg agatcctgcc gaccatcgtg atcgagcgag
      361 tggtcgattt aggattttct gcgatcgtgc ttcttgcggc gctgccattt gtggtcggag
      421 cagagggcgc cgggcagatc ggcatcacgg ttggaatcat tgtctttatc ggaatcgtaa
      481 tgctttacgt gcttgctcgt tacaatcaat gggctctgga tatttttcat aaactcagtg
      541 cacgctggcc ctccctgcag aagtttggcg gaagtttcct ggagtctttc tttgcggggc
      601 tgggtgtgct gacagatgga tggttgttcc tgcgttttct gttttggatg accctcaact
      661 ggggaattgc catcttctct tattacttaa tcatcctggc atttttcccc gaagcacaac
      721 cggtgtgggg aatgtttggt cttggcgcgg cggcgtttgg cggtgcgatc ccttctttgc
      781 caggagccgt tggaacgttc gaaggtgcgt tcggcggagc gctcaccttg ctgaccggcg
      841 atgaatccac ctcactggct gtcgccttga cgggtcgcct ttacaattac ataaacagtg
      901 cggtgatcgg cggtattggc ctggtacgtg aaggtcaaac cctctcagga atctatgaac
      961 aaatacggaa tttcaggacg agaagtaatc aggcatgatt ccgctgtatg acacgattcg
     1021 cccgcgacaa ttccctttta tcaactggct tctcattctc ttcaatgcgg tggtgttctg
     1081 gtatgaactt cagttaggtg agtcggcgct tcaagacttc atctttacct ggggactcgt
     1141 tccggcagag ttgttatcca atccatcaga ggaatggcag acgattttta catccatgtt
     1201 tttgcatgga ggctggttcc atgtgatcag caatatgtgg gtactctata tctttggcga
     1261 caacatcgaa gaccgggtgg gaagcatacg atatctgatc ttctacctat tgtgcggaat
     1321 cgccgcggct gcccttcaga tttttgttat gcaaagtagt gccgttccga tgatcggcgc
     1381 aagtggtgcc atcgccggtg tattgggtgc ttatctcata tcgtttccgc gttcacggat
     1441 tgccagcctt gtaccgattc ttttcatttt tacgatcatg gaagtaccgg ctttcctttt
     1501 tctgatcttc tggtttgtct cccagcttta ttccggattg tttgccatgc agggaggggg
     1561 agcgagcggc attgcctggt gggcacacat cggaggattt gtttttggtc tgattgcagt
     1621 atcgttcttt cgcagaagga tcatgtatcg cggttattaa aaataaatgt cattgtgagc
     1681 cctgctggtt gagacgcaag cagtcgaaac caaagggcga agcaatcccc tgtttcgagg
     1741 acgctttgcg gcttcctcgc aacaaccgct cctcgcaatg acatgtatgc aacaggagaa
     1801 atatggctcg aaattatttt gtcaccgggg gagcggggtt tattggctca aactacgtcc
     1861 acaggctggt agagcgcggc gaacaggtaa ctatctttga caatctctct cgcggcggcg
     1921 cgccgcgtaa catcgcctgg ctgcaggaaa cgttcgggaa tgacgccttc aaactgatcg
     1981 tcggagattt gcgagatgcg gacctgatac aggaatccgc caaagacgcg gatgtcatcg
     2041 tccatctggc agggcaagtg gcagtcacta catccgtaaa taatccaaga gatgattttg
     2101 aagccaacgc attgggaacg ttcaatgcat tggaagccgc acgcttatct gatagaaacc
     2161 cggtcttcat ttattcatcc acgaataaag tgtatggcgg catggaagat gtggaaatta
     2221 tcgaggatgc cacccgttgg ttgtacaagg atctggaata tggttgctct gaagcacaac
     2281 cccttgattt ccacagtcca tatggagtaa gcaaaggcgc tggcgatcaa tatacccgtg
     2341 attactcccg tatctatggt ttgcgctccg ttgttttccg acaaagctgt atctacggtc
     2401 cgagacaatt tggcgtggaa gatcagggtt ggctggcatg gatgatgatc gcggcagtca
     2461 ctggaaaaca gatcaccgtg tatggcgacg gtaagcaggt acgtgatgtg ctgcatgtga
     2521 atgacctgtt gaatgcctat gacctggcag tcgagaagat cgacgacgta aaaggagagg
     2581 tgtataacgt cggtggcggc acacgtaacg tgatggcgat ctgggcagaa tttggcccaa
     2641 tcctggagag attgatcggc aggaagatcg aagtcgcgcg cgatgattgg cgacccggtg
     2701 accagcgcgt cttttacgcg gactttcgta aagcgcagcg cgaacttggc tgggaaccca
     2761 agatcgatct cgaagaggga atggaattgt tatttgattg gatcaatcac aacagagagc
     2821 tgttctaggc attacaggta tcattaaaat tcagacccta aaggttttat tagcaaacat
     2881 gtgtaaaatt tgatgggaat ctcagtttgg ccttggtctt tgcatgaaat aaaaccttta
     2941 gggtgtctat cttttcttga gagaacaatt tttctcataa catgctatga atctacgact
     3001 taatcgccgc gattttctga aactggcagg cttggtgcct ttaagccttt ctgccacgcg
     3061 cctgttgcaa agccttgatg gcacacaacg gcttcaggga acgcaaaaga acgtgattgt
     3121 gattgttttc gacgcgttct cggcctacga tatttcactt tatggatttc ctcgtgaaac
     3181 aacgccgaat atcaaccggc tggccaaacg tgccgttgtc tatcacaatc actttgccgg
     3241 agggaacttc accacgccgg gcgcgggatc gctgttgaca ggcgtgcttc cctggacgca
     3301 tcgggcgttc aatcacaacc agaaggtggc aactcctttt atagacaata atatctttag
     3361 tgccttcaag gactattatc gcgttgccta tactcacaat ggatttgcga atacgttgct
     3421 caagcagttt catgaataca ttgatgagtt cattcctcga gaaaaatact ttcttgagtc
     3481 cattgacact ttaatttcag ctttgttcaa aaatgatgat gacatcgcat ccataagctg
     3541 gctgcgaagt atgagaattg aggagaacgg tggcttcgcc tattccctgt tcctctcgca
     3601 tatttacgag gctattcagc aaaagcgaat tgaagatctt aagaaagact ttccaagagg
     3661 aattccaact actggtgcag attatggcta tctattggaa actgccatag acgcggtcgg
     3721 gaacaggcta tctgaaatcc ctcagccttt cttcggatac ttccatttca tgcccccgca
     3781 tcatccgtat cgcccaccgc gcgagtatgt caacgcgttc aagggtgatg gtttcaaaga
     3841 tgttgaaaaa ccggtggaca ttctggtcag gcaggaagtc aaaaacctgc cgatgaaacg
     3901 cacggaatat gacgagttca tcctgtactg tgatcgcgag tttggcaggc tatttgatca
     3961 actggaatct tccggattac tggaaaacac ctggctcgtg ctaacctctg atcacggcga
     4021 gatgttcgag cgcggcttcg ccgggcatgg atcaaaactt ttgcatcagc ccgtggtgcg
     4081 gattcctctg atgatctttg aaccgggacg cgattctgga atggacattc acgaatatac
     4141 aagcgctgtt gatgttttgc cgaccttgac tcacgttaca ggaattaata cacccggctg
     4201 gggagagggt atagttttac cgccttacgc aactacctcg acaggatcga atagaaatat
     4261 ttatgtggtg caggcaattg acaactcgcc aacggccccg ctgacagaag cgtctacaac
     4321 gctgatcaag gaaaactata agcttcatta ctacttcggc tatccagagg cagggaatgc
     4381 tgaaattgta aaactatttg atatccaagc tgatcctgat gaactgatag accttgcttc
     4441 atcgaagagc gggattgcgt cggaactgct cgatgaatta caacgcaaat taaaagaagt
     4501 aaatgaaccg tatctttaac ctccatttgt c
//
LOCUS       DASTJP010000250         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1460237, whole genome shotgun sequence.
ACCESSION   DASTJP010000250 DASTJP010000000
VERSION     DASTJP010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460237"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..329)
                     /locus_tag="VFQ23_07660"
     CDS             complement(87..329)
                     /locus_tag="VFQ23_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET9906500.1"
                     /translation="MLRKIFRTGNSIVVSIPKDILDKLKLSEGDDVSVELDSKQRQIV
                     ISPVEKPLAVGVDETFARQVDEFIKEYRPALEALAK"
     gene            complement(603..824)
                     /locus_tag="VFQ23_07665"
     CDS             complement(603..824)
                     /locus_tag="VFQ23_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET9906501.1"
                     /translation="MSHKLSPIHPGEVLVEEFIKPMGLSQNRLAINIGVDARRIYEIV
                     LGKRAITADAALRLSRFFGLDQIAVAIRS"
     gene            complement(894..2087)
                     /locus_tag="VFQ23_07670"
     CDS             complement(894..2087)
                     /locus_tag="VFQ23_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9906502.1"
                     /translation="MSHELVLRQKAIRWKQAGRAVSWICQRLEHSREWFYKWWNRYQE
                     EGARGLRDRSHVAQSHPHRWSGEIRQAILDIRDRLMRRRGPRARYRLAGAATIRHELA
                     CLGYDPLPSLRTVERALHQGGRTSPAFRPEPCANSSSYPTIRLTHSNQRHQLDLIGPR
                     YLKGSRRQWYFLVYRDVYDGAVFVEFQPKSRMEGVLAFVVRAWQRLGLPDTLQVDNSD
                     LFGLTSHPGSLNRFIRLALLVGVNLTFIPEHEPWRNGAIENFNGWLQERILAIPLRSP
                     SQVRRELNTMMDVCFHEHIHPHLNFQTTAQVRKHLSPRTLPHNFRRHLEPLPVAIGRV
                     TFLRRVRSSGRITILGVKFKIGKRLSHQYVSAVLYTRTMILKIHSRRRLLKQFDFPFI
                     GKLKL"
     gene            2285..2572
                     /locus_tag="VFQ23_07675"
     CDS             2285..2572
                     /locus_tag="VFQ23_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906503.1"
                     /translation="MQFVPYRDLRNEPSALRKKLAAEGELIVTVDNKPFAVMINLGDE
                     DVQDILLMVSRLRAQMAARSIRSQARQEGLNKMSLKEINTVIKKTRAERKP"
     gene            2572..>2948
                     /locus_tag="VFQ23_07680"
     CDS             2572..>2948
                     /locus_tag="VFQ23_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HET9906504.1"
                     /translation="MLTVVRDTNVLVSSLLSQKSWPAKILDLVLSDHIQVAYDDRILR
                     EYEEVLSRPEFSIHPSRMLAVIDHIELTGKFVEPEPLSTEGYTDPDDIMFAEVFITSN
                     ADALITGNLRHYKPLLKENALVLS"
BASE COUNT          758 a          736 c          761 g          693 t
ORIGIN      
        1 aagatctcga agaccatgct caccaccagt ttcacttacc aggcgataat gaataaaaag
       61 aatctgttct gctgtcaggt aagtgatcac ttggccaatg cctccagggc agggcggtac
      121 tccttgataa attcatccac ttgtctggca aaagtttcgt ctacaccaac tgccagtggt
      181 ttttcaaccg gagagatcac aatctgacgc tgcttactgt caagttccac tgacacatca
      241 tcaccctcag ataatttcag cttgtcaagg atatctttgg ggattgaaac cacgatgctg
      301 ttaccggtcc taaatatctt tcgtagcatg ctgcctccag ttctgtaatt acagtgtaag
      361 tataccattt tgtccgggga attgctttca gcgggcttag attttcaact tagccatgaa
      421 actggtattg agtaattcaa agggaacaag gataattatc gagtaaaccc ggcctttcat
      481 gtcggtaggg gacttaaaga acagagagcg atcccataag atcgctctcc agttcttatt
      541 gagccatagc gtagggacgt acttcacgtt ctagccgttt cccaagttga tcctgcgcaa
      601 tttcaagatc gtattgcgac tgcaatctga tccaagccaa agaagcggga aagacgtaag
      661 gcagcgtcgg cggtaatcgc gcgcttgcca agcacaatct cataaatccg gcgcgcatcc
      721 acgccaatgt taatagcaag ccgattctgg cttagtccca tcggcttgat gaattcctct
      781 acgagtacct cacctgggtg gataggagaa agtttatgac tcatattttc ttcctgccgt
      841 ccgccacatg agtgctgacg acagttggaa aaagataacc gccagatgat tgcttacagt
      901 ttgagcttgc cgatgaaggg aaagtcgaac tgcttgagta ggcgacggcg agaatggatc
      961 ttgagaatca ttgtgcgagt gtaaagaact gccgagacat actgatggga cagacgtttg
     1021 ccaattttga atttcacgcc caggatcgtg atgcgacccg aggaacgaac gcgccgaagg
     1081 aaggtgacgc ggccgatggc gacgggcaag ggttccagat gtcgacgaaa gttgtgaggc
     1141 aacgtgcgcg gcgacaggtg tttccgaact tgcgccgtcg tctgaaaatt gagatgcgga
     1201 tgaatatgct cgtgaaaaca aacgtccatc atcgtattca attcgcgccg gacttgagag
     1261 ggcgaacgca agggaatggc caggatgcgc tcctgcaacc aaccgttgaa gttctcgata
     1321 gcgccattgc gccagggttc gtgctcgggg atgaaggtaa ggttgacccc gaccagcagt
     1381 gccaggcgga tgaagcggtt gagcgaaccg ggatgggagg tcaagccgaa gagatcgctg
     1441 ttgtcgacct gcaacgtgtc gggcagaccc agccgctgcc aggctcgtac cacaaacgcc
     1501 agaacccctt ccatgcgaga ttttggctga aattcgacaa aaacagcccc atcgtagacg
     1561 tcccggtaga ccaggaagta ccattgccgc cgcgagcctt tcaggtagcg cggcccgatc
     1621 agatccaact gatgtcgttg gttgctgtgc gtcaggcgga tcgtggggta gctgctcgag
     1681 ttggcgcacg gttcgggtcg aaatgctggc gaggtacggc caccttggtg cagcgcgcgt
     1741 tcgacagtgc gcagcgatgg caacggatcg tagcccagac acgccaattc gtggcggatc
     1801 gtcgcagctc ctgccagacg atagcgtgcc cgcggaccgc gacgtcgcat caagcgatcg
     1861 cgaatatcca ggatcgcttg acgtatttcg cccgaccaac ggtgcggatg gctctgcgct
     1921 acatgcgagc gatcgcgcag tccgcgggct ccttcctcct gatagcggtt ccaccatttg
     1981 taaaaccatt ctcgactgtg ttccaatcgc tgacagatcc agcttaccgc tcgacctgct
     2041 tgtttccaac ggatcgcttt ttgtcgcagt actagttcgt gagacatcgc agcacctccc
     2101 gaaagaccag cttgattttt cggaggatac tgtcagcgat cctgtggcgg ttatcttttt
     2161 catctgtcag caatcacgtg gcggttagca catagaatct aaaagctgtt ccaggagacg
     2221 ttcaatagag cacgaaataa tggtaaaata ccgtagcgaa atttgctacg aaaggagtat
     2281 ttccatgcaa ttcgttcctt atcgggattt acgaaatgaa ccgtctgcct tgcgcaaaaa
     2341 actggctgct gaaggtgagc tgatcgtgac tgtcgacaat aaaccgttcg cagtaatgat
     2401 aaatcttggc gacgaagatg ttcaagatat attgttgatg gtttctcgat tgcgcgccca
     2461 aatggccgcg cgttccattc gcagccaagc acgccaggaa ggtctcaata agatgagctt
     2521 gaaagagatt aacaccgtaa taaaaaagac ccgtgctgaa cggaagcctt gatgttaaca
     2581 gtcgtacggg acacaaacgt gctggtcagc agcctgctca gtcagaaaag ctggccagca
     2641 aaaatccttg atctggtttt gagcgaccac atccaggttg cctatgatga tcgaatcctt
     2701 agagaatatg aggaagtcct ctcgcgacct gagtttagta ttcatccatc cagaatgcta
     2761 gcggtcatcg atcacatcga actcacaggt aaatttgtag agcctgaacc attatcgaca
     2821 gaaggatata cagatcctga cgatattatg tttgctgagg tctttatcac ctccaatgca
     2881 gatgcactca tcacaggaaa cctccgtcac tacaagcccc tgctcaaaga aaatgcatta
     2941 gttctctc
//
LOCUS       DASTJP010000251         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_111323, whole genome shotgun sequence.
ACCESSION   DASTJP010000251 DASTJP010000000
VERSION     DASTJP010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111323"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFQ23_07685"
     CDS             complement(<1..629)
                     /locus_tag="VFQ23_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9906505.1"
                     /translation="MNFIDTNLLSLILFFPTFAALVMLFLPKNENQLFRWFAFVASLV
                     PLVLSLIAWFRFQPSQPGFQFEESYVWYEAIGSSLHLGVDGLSLTMVLLTTLLTPLAI
                     LASFNINDRVKAYMMLFLFLETGMLGVFMALDLLIFFVFWEVGLVPMYFLINQWGSEN
                     RNYASLKFMIYTMGGSLGLLLAIQLLGVLFGTYDLVTLYQQWTSLDGNST"
     gene            complement(639..>2034)
                     /locus_tag="VFQ23_07690"
     CDS             complement(639..>2034)
                     /locus_tag="VFQ23_07690"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9906506.1"
                     /translation="SGFFGFSAAGLIGLLLFIGTVGKSAQFPLHVWLPDAMEGPTPVS
                     AMIHAATMVSAGVYAVIRMFPLISLDPLTMSIVAFIGAFTALFAATIAVAQNDVKRVL
                     AYSTISQLGYMIAAIGIGAYVAAAFHLITHAFFKALLFLGSGSVIHGMEHGVLHTGNH
                     HVDPQNMFNMGGLRRKMPITFWTFLIGGFALSGFPLVTAGFWSKDEILADAFGHGHWW
                     VFGTLAFAAFLTAFYTMRQITLTFLGEPRTPEAEHAQETPWTMTAPLVILSVFAIGFG
                     WLGIPEHFPVLGGLLPNWIHDFVGGTLAEHPEAVEFNIFPLLTSLAVALGGLYLGWLT
                     YRSVNSPAEDRWQFPVLKNKYYFDELYTSLFVKPAFWLAETFIYKWMDKGFIDGILHV
                     FDPLTNGIGTFIRTRFDLPFINRFLGDGSADVTYWFGGKLRFLQTGRIQQYLMYALVA
                     SIVIGAAIYYFLLA"
BASE COUNT          631 a          508 c          507 g          388 t
ORIGIN      
        1 gttgaattgc catccagaga cgtccactgc tgatataacg tgaccagatc ataggtcccg
       61 aagagaacgc cgagtaactg gatcgccagc aaaagtccca gagatccacc catcgtgtag
      121 atcataaatt tgagcgaagc gtaattccgg ttctcgctcc cccactgatt gatgaggaaa
      181 tacatcggca cgagacccac ttcccagaac acaaagaaga tcaacaggtc cagcgccata
      241 aacacgccca gcatccctgt ctcgaggaac aggaacagca tcatgtaggc tttgacccga
      301 tcattgatgt tgaaggatgc gagaattgcg agcggcgtca ggagagtggt caataagacc
      361 atggtgaggg aaagtccatc aacgcccaga tgcaacgacg aaccgatcgc ttcataccag
      421 acgtatgatt cttcgaactg aaaacctggc tggctcggtt gaaatctgaa ccatgcaatc
      481 agcgaaagaa ccagcggaac caggcttgca acaaacgcaa accagcggaa gagctgattt
      541 tcattcttcg gcaggaatag catcactagt gcagcgaagg ttgggaagaa caggatcagg
      601 gaaagaagat tcgtatcgat gaaattcatc acatgggcct aggcaagaag aaaataataa
      661 atcgcggcgc caatgacgat actggctact aaggcgtaca tgagatactg ttggatacga
      721 cccgtttgaa ggaaccgcag tttgccacca aaccaatatg tcacatctgc cgagccgtcg
      781 ccgagaaagc gattgatgaa tggaagatcg aacctggtac gaatgaaggt cccaattcca
      841 tttgtgagag ggtcaaagac atggagaata ccgtcaatga atcctttatc catccactta
      901 taaatgaatg tctccgccaa ccaaaacgca ggcttaacga agagcgaagt atagagctca
      961 tcaaaataat atttgttttt aagaacggga aattgccatc gatcctcagc gggtgagttc
     1021 acgctgcgat aagtaagcca accaagatac aaaccaccca gcgcgactgc taaagaggta
     1081 agcaaaggaa aaatattaaa ttcgacagct tccgggtgct ctgccagggt gccaccaaca
     1141 aaatcgtgaa tccaattcgg cagtagtcca cccaaaacgg ggaaatgttc agggatgccc
     1201 agccagccaa agccaatagc gaagacagaa aggatgacca gcggcgcggt catcgtccag
     1261 ggagtttcct gtgcgtgttc cgcttctgga gtgcgaggtt cgcccaggaa ggtcagagtg
     1321 atctggcgca ttgtgtagaa agcggtcagg aaagccgcaa aagccagcgt tccaaatacc
     1381 caccagtgac cgtgaccaaa cgcatctgct aatatttcat ccttcgacca aaaaccggcc
     1441 gttaccagcg gaaaccctga aagggcaaaa ccgccaatta agaacgtcca aaacgtgatc
     1501 ggcatcttcc ggcgcaatcc acccatattg aacatgttct gcgggtccac atgatgattg
     1561 ccggtgtgca gtacgccatg ttccataccg tgaatgacgg aacccgaccc aaggaaaagt
     1621 aatgccttga aaaaggcatg tgtaataagg tggaacgcgg ctgccacata cgccccaatc
     1681 ccaatggcgg cgatcatgta tccaagctga gaaatggtgg aataagctaa cacacgcttg
     1741 acgtcatttt gcgcaacggc gatcgtcgca gcaaagagcg cagtgaacgc gccgatgaat
     1801 gcaacaatgg acatcgttag agggtcgagg cttatgagcg ggaacatacg aatgaccgca
     1861 tataccccag ccgaaaccat ggttgcggcg tggatcatcg cactgacagg tgttggacct
     1921 tccatcgcgt ccggtaacca cacatgcaga ggaaattggg cggatttacc gactgtacca
     1981 ataaaaagca ataatccgat cagcccggct gctgaaaatc cgaaaaatcc acta
//
LOCUS       DASTJP010000252         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_149816, whole genome shotgun sequence.
ACCESSION   DASTJP010000252 DASTJP010000000
VERSION     DASTJP010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149816"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..659)
                     /locus_tag="VFQ23_07695"
     CDS             complement(51..659)
                     /locus_tag="VFQ23_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906507.1"
                     /translation="MSKKKKITFADVLWKIIVKPAWYWLVTIIPTAIAMYQFFGGDRN
                     TIANISSRIWLVVAIIAFLLTIVISTFIFRNSRLLLHDKTKRSGRSRNKKALSAKSDA
                     KDGGQSVAISGNNYAPINQGSNIAPQYDHETQPNIEVTKFGSEVLTLKLINKIAGQAI
                     QIGVGETLERFYIEFVNRKKTGVKTEDAIKVYVNISFLHSRL"
     gene            985..2409
                     /locus_tag="VFQ23_07700"
     CDS             985..2409
                     /locus_tag="VFQ23_07700"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET9906508.1"
                     /translation="MSKQTLVMKFGGTSVGSVDALTKATQIIKDAREEYPRVVVIASA
                     MAGVTDLLLKSASLAAQGNVDSLPTTESTLREKHFSATEALIKEAGLRDQTKQEINAL
                     ILLLVNLCNAIAVLGEASPRALDAVASLGERMSVPLLSAVVQSSGIKARGIESSEFIL
                     TNSHFQNAHPDFEATTEKTRRLLNPLLDDGIVVVTTGFIGATPEGVITTLGRGGSDYS
                     AAIIGSVLPADEVWIWTDVDGVMTTDPRLAPEARTLPEISYSEIAELAYYGAKVLHPK
                     TIRPVVEAGIGLRICNTFNPSHPGTRLTANSKSNGKPVNGKVVKAVTAIRKQKLVTIE
                     GRGMLGVPGVAARAFGAVAATGTSVPLITQASSEQSICFAVPSETAASVLASLENTFA
                     YEIEEEDIDRVWSTDDVSIITVVGAGMRHTPGVAGQVFSQLGNNGVNVLAIAQGSSEV
                     SISLVVDAVDTENAVKALHQLIIN"
     gene            2576..>3206
                     /locus_tag="VFQ23_07705"
     CDS             2576..>3206
                     /locus_tag="VFQ23_07705"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET9906509.1"
                     /translation="MSSSQIPVAVLGATGSVGQRFMSLLDNHPWFKVVALAASDRSVG
                     QKYSAAARWVLDTPMPDYAKDMIVVPATTEAVQARIVFSALHSDVAKDLEPQFAQAGV
                     AVCSNASSYRRGEDVPLLLPEINAEHIHLIKEQRKRRGWSGAILTNPNCTSTGLAIAL
                     KALDNTLGVKKVIAFSMQALSGAGYPGVPSLDIMDNVIPNVANGGEEDKV"
BASE COUNT          814 a          777 c          792 g          823 t
ORIGIN      
        1 acggatcata tttcacccac catctgggtt tatcatgaga attgatcaac ttacagtctt
       61 gagtgtaaaa aagaaatatt tacataaacc tttatggcat cctctgtttt taccccggtt
      121 ttctttctgt tcacaaattc tatataaaat ctttctagag tttctcctac tccaatttga
      181 atcgcttgac ctgcgatctt attaattagc ttaagtgtta atacctcact accaaattta
      241 gtaacttcaa tattgggttg ggtttcatga tcatattgag gcgcgatatt acttccttga
      301 tttatgggtg cataattgtt tccactaata gcaactgatt gtccaccatc ttttgcgtct
      361 gatttagccg ataatgcttt cttattacga cttcgtccac ttcttttagt tttgtcgtgc
      421 agtaacaatc tcgaattacg gaaaataaat gtgctgatca caatagttaa aagaaacgct
      481 attatagcga cgactagcca tattctcgac gaaatgttag caatcgtgtt cctatctccg
      541 ccaaaaaatt gatacatagc gatggcagtt ggaataattg taacaagcca ataccatgca
      601 ggtttaacta taattttcca aagaacatca gcgaatgtaa tcttcttttt cttggacatc
      661 catcatgatc ccactacatt ttactcaaac gtaggatgtt aaggaggatg gcaatcaatt
      721 catatatccc cttgacgcat tctcccctct agcatagaat accgcccaac atgcaaagaa
      781 ctttgcacaa gcacggctta ggtactatga ccatgactac tacctccggt gatcaccgtg
      841 aggcaattgt gcatgggcag aagtaactga gtcaagagtt atctcgaaac acacagcgcc
      901 tcacggaaac gtggggcgtt tttgttaccc cacccccaac ccctccccac gacttcgtcg
      961 tggagagggg agaaagacaa aattatgtca aagcaaacat tggtgatgaa attcggtggt
     1021 acgtcggttg gttcggtcga tgccctgacc aaggcgactc aaatcataaa agatgcgcgc
     1081 gaggagtatc cacgcgtggt ggtgatcgca tccgccatgg cgggtgtgac tgatctgttg
     1141 ctgaagtctg cgtctctggc cgcgcaggga aatgtggatt cgctcccaac tactgaatct
     1201 accttaaggg agaagcactt ctccgctacg gaggcgctca tcaaagaggc aggacttcgc
     1261 gatcaaacga aacaggaaat caacgcgttg attctcttgt tagtaaactt atgcaatgcg
     1321 atcgcggtgc ttggcgaagc cagtccacgc gcgttggatg cagttgcatc gctgggcgag
     1381 cggatgagtg ttcccttgct gagcgcggtg gtccaaagct cagggattaa ggccagggga
     1441 atcgagtcca gtgaatttat tctcacgaat tctcattttc aaaacgcaca ccccgatttt
     1501 gaagccacta cagaaaagac ccggcgatta ttgaatccgc ttttagatga cggcattgtc
     1561 gttgtcacaa ccgggtttat cggcgcgacg cccgaaggtg tgatcaccac cctggggcgc
     1621 ggagggagtg attattccgc ggctattatt ggttcggtat tgccagccga cgaggtctgg
     1681 atctggacgg atgtagatgg tgtgatgacc accgatccac gccttgcacc agaagcgcgc
     1741 accctgccag agatcagtta tagtgaaatc gcagagttgg cctattatgg tgcgaaagtt
     1801 ttgcacccaa aaacgattcg ccccgttgtg gaggcaggaa ttggtcttcg catttgcaac
     1861 acgttcaatc catctcatcc gggcacgcgc ctcacagcaa attcgaaaag caatgggaaa
     1921 cccgtcaacg gcaaggttgt caaggcggtg acggccattc gcaaacagaa attggtgacg
     1981 atcgagggac gcggcatgtt gggtgtgcct ggcgtggccg cgcgtgcttt tggcgcggtt
     2041 gcagcgacag gaaccagcgt cccgctgatt acacaggctt cttcggaaca gtcgatttgt
     2101 tttgcggtgc cttccgaaac tgcagcttct gtgcttgcat cccttgaaaa cacattcgcc
     2161 tatgaaatcg aggaggaaga tatcgatcgt gtctggtcaa ccgatgacgt ttcgatcatc
     2221 accgtcgttg gcgcagggat gcgtcacaca ccgggagtgg caggccaggt ctttagccag
     2281 ctgggaaata atggtgtcaa cgtgcttgcc atcgcgcagg ggtcatcgga ggtctcgatc
     2341 agtctggtgg tggatgctgt tgatacagaa aacgcagtca aggcgttgca tcaattaatt
     2401 attaactaaa cctcggtggt cgagtagccc tgagcggttt tcgcgaaggg catatcgaga
     2461 ccaccaagtt gaattcaaat aaaagtatct tgaaaatctg gtggtttcga tacgcccctc
     2521 aaagagcatt cggggctact caaccaccga tgtaaaaaac aaaggagaaa tcatcatgtc
     2581 ctcatcccaa atccctgtag ccgtgctcgg tgcgaccggt tctgtcggtc agcgattcat
     2641 gtctttgctg gataatcatc cctggttcaa ggtggtcgcg cttgccgcgt cggaccgttc
     2701 cgttggacaa aagtattcag ctgccgctcg ctgggtgctt gacactccca tgccagacta
     2761 tgcaaaagat atgatcgtcg tgccggcaac aacggaggcc gttcaggcac gaatcgtttt
     2821 ctctgcttta cacagcgatg tcgccaaaga cctcgagcca caattcgcgc aggcgggcgt
     2881 tgcggtttgc tccaacgcat cctcttatcg acgcggcgaa gatgtgcctc tcctcctccc
     2941 ggagatcaat gcggaacata ttcatctgat caaagagcaa cgaaagagac gcggctggag
     3001 tggagctatt cttacgaatc cgaattgcac cagtacaggc ctcgcgattg cacttaaggc
     3061 acttgacaat acacttggcg ttaaaaaagt tatcgcgttc tctatgcagg cgctatcagg
     3121 cgcgggctat cccggcgttc cgtcgcttga tatcatggat aacgtgattc cgaatgttgc
     3181 aaatggcggc gaagaagata aggtgg
//
LOCUS       DASTJP010000253         7767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1378222, whole genome shotgun sequence.
ACCESSION   DASTJP010000253 DASTJP010000000
VERSION     DASTJP010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378222"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..754
                     /locus_tag="VFQ23_07710"
     CDS             74..754
                     /locus_tag="VFQ23_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9906510.1"
                     /translation="MTVNSGFRKAKDMPCNCCEITDNTFGEAEAKSNLKMYRRRGPAK
                     QTRLILGAIRSLGIKGAALLDIGGGIGAIHHELLKDVAREATHVDASSAYLKEARKET
                     ERQGNTERVKFVHADFTDAASDLPPADVVTLDRVVCCYPDFRGLLKAAADHSQRVIAL
                     TYPRETWYMRIGFGVMNFFQRLMKDPFRVFLHPVSEMESLLKKEGFVKVTVQRLFIWE
                     MAMYQRTS"
     gene            1221..2069
                     /locus_tag="VFQ23_07715"
     CDS             1221..2069
                     /locus_tag="VFQ23_07715"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9906511.1"
                     /translation="MFKIGEFSQLSRVSVRMLRHYDQLGLLKPSRTDTFTNYRYYSAD
                     QLPRLNRILALRDLGFSLEQIGNMLDEDLSAEQLTGMLKLKRAEIEQQMQVEQLRMAR
                     LEARISQMNGQTDPGQYDVVVREIEPQLVATYRELAPDDDRIQYMFEELEAYVTQFNA
                     RADKPPMSIYHDPEYREENIDAEVVVPLKSAIPGTDVFHVLEMARIPKAACVVHTGEY
                     ATIYQAYNALLRWIEANGYRMKGPIREVYLSYSADGLGFELPPTYLVKESNPFVTELQ
                     LEIEKD"
     gene            2174..2512
                     /locus_tag="VFQ23_07720"
     CDS             2174..2512
                     /locus_tag="VFQ23_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906512.1"
                     /translation="MAYYLVKAKYHKHLLAELRTRLDSGEINKMRPFGTALQYSLDNA
                     RLDPKGEGWSAWEEEDYCSPPLAQERAAVLDTYFTSLSVEKVSQGNGWSRIESLPRLW
                     KKSNGGTDAL"
     gene            2502..3527
                     /locus_tag="VFQ23_07725"
     CDS             2502..3527
                     /locus_tag="VFQ23_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9906513.1"
                     /translation="MRYKLLGRSGLRVSELCLGTMTFGDDWGWGAPKEESRKMFDAFA
                     ESGGNFIDTANNYTNGTSEKFVGEFIASDRDRFVVATKYTLSERAEDPNYGGNHRKNM
                     FRSIEGSLKRLNTDHIDLLWLHMWDGVTPIEEVMRALDDLVRSGKVLYVGISDTPAWV
                     VSQANAIAELRGWSRFIAYQGEYSLASRAPERDIMPMTQALEMAFTPWGILEGGEMTG
                     KYNAPSDEPKRSSDTSPRIKSLAAELMSLAEEIGRTPSQIAINWVRQQPYLVVPIIGS
                     RSEKHLRDNMSCLEFELTPDQIKRLSAASPIDLGFPHSFLASDHVRGLIFGKTFARID
                     NHRVRVG"
     gene            complement(3608..4990)
                     /locus_tag="VFQ23_07730"
     CDS             complement(3608..4990)
                     /locus_tag="VFQ23_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9906514.1"
                     /translation="MDNLNSLTITSALEFIQAQKATARDFADACLSRIERLNPMLNAF
                     ITVIKEQPTAAISASTPSHTITNMLRGIPIAVKDLFDTTGIRTTAGSRFFSEHIPETD
                     AFVVEKLKQAGATIIGKTNTHEIALGVTGNNPHYGTARNPWDTTRISGGSSSGSAIAV
                     AADMALGALGTDTGGSIRIPASLCGVVGFKPSYGRVSLRGVFPLSWNLDHAGPLTKTV
                     KDAALLLQVISIYDPRDPASIKLLTGDYLSHLTDAVGGKKIAFGVGEYVEGADSEVLE
                     AVKEARRVFESLGCRLEDVNVDWMRDAALANKTMTQSDGAAVHRDRLKEHPDWFGDDI
                     RQRLEAGAKTTSTEYILARRTQTEVRRRCEQFFEAYDFLILPTTPNSAPAVEGADALE
                     QAARLTRFTAPFNLTGLPALSLPCGFTRDGLPIGLQIVSRAGADAKVLNAGYAYEQET
                     EWHKQKPNLN"
     gene            5022..6587
                     /locus_tag="VFQ23_07735"
     CDS             5022..6587
                     /locus_tag="VFQ23_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906515.1"
                     /translation="MQSVSFSKNRFWQFLFILSVIGIILCWVATFRFGAGVAADSTKY
                     LSVAESLLAGEGLRDHLDMPLLAWPPLYPILLAALSRLTGLDVFVVGWYFNVFLAGLN
                     IFLSGIIFCRVFSEKPLYAYLGSLFVLLASSSLRMHATISSDPFYLTLTLGFLIAVDR
                     YIKRHSYSAFLWMIVFSVLAPLQRYVGPAVCVTAVLVILIEKRKSIRVLLRDSILLGV
                     ASILPIAWWLLIHNIMTYGSLWGSTGDRTVDVLKNTELALTKMLHWFVPYLSFLMPIL
                     NRPLILLSVMALVIFLLNRKNKGSGRLWINLLAAPSAYPSLIYAVVYFAAVAVTIVTI
                     VHRDLFSDRYYVMLLVPTVIFLFITFDTLIRPLLAASYTRFSYILILIFALWSVYPIY
                     TISEYLANASTQGEPSGFNMYNNRTYRDMDLVGEMQRLREDQPAAIFYSNYVDAVWFY
                     TGKPVALLPLANVSSPSEAYAGWPGDKPGYIVWFEPNEYKHYLSPEKIAEFASVQLVF
                     EGEGGKLYYVQPR"
     gene            complement(6617..7474)
                     /locus_tag="VFQ23_07740"
     CDS             complement(6617..7474)
                     /locus_tag="VFQ23_07740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906516.1"
                     /translation="MIHQVDFIEEQGIFVPRVRPKHRNDEYDEMGFEVLIRMQREHFW
                     YRGRHKLLLHALEKEIPKHLGQRTGLRAIDMGGGCGGWLEYIHTHRPGMFHKIALGDS
                     SMHALSLAEPVVGGFATRYQIDLLNLAWAETWDVVFLLDVLEHIPDHEEVLRQIYRSL
                     RPGGLLFVTTPALNFFWTYNDDLALHQRRYSRQDFQILAEQTNFELLRTNYFMFFLSP
                     ALFLSRMLFRPPKLATPEQLKAHLVQTHRVPSQPINGLLTKLFSFEASMINAVNFPWG
                     TSILAVLKR"
     gene            complement(7603..7755)
                     /locus_tag="VFQ23_07745"
     CDS             complement(7603..7755)
                     /locus_tag="VFQ23_07745"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET9906517.1"
                     /translation="MLIRDIRASVGAGFLYPLLGEMRTMPGLPTRPSYYDIDLDLKTG
                     KVVGLF"
BASE COUNT         1927 a         1813 c         2007 g         2020 t
ORIGIN      
        1 gaacaaatac tatcgcaatt aagttgacag tcacttttgc gaagcatccc cgaagcatag
       61 ctgaggggac atagtgactg tcaactccgg ctttagaaag gcaaaagaca tgccatgtaa
      121 ttgctgtgaa ataactgaca acacgtttgg ggaagccgaa gccaaatcga acctgaagat
      181 gtatcgcagg aggggtcctg cgaaacagac gagattgatt ttgggagcta tccgttcatt
      241 gggaataaag ggcgcggctt tgttggatat cggtggggga atcggcgcga tccatcatga
      301 gttgctaaaa gatgtcgcgc gcgaagctac ccatgtggat gcatcatctg cttacttgaa
      361 ggaagcgcgg aaagaaacag agcggcaggg caatacggag cgtgtcaaat ttgttcatgc
      421 ggattttaca gatgccgcgt cagatttgcc tccagccgat gtggtgacgc ttgaccgagt
      481 cgtctgctgc tatcccgatt tccgcggctt gttgaaagcc gcagcagacc acagtcagag
      541 ggtgatcgcc ctcacctacc cacgcgaaac ctggtacatg cgaatcggat tcggtgtcat
      601 gaattttttc caacgactca tgaaagatcc gtttcgcgtt tttctgcatc cggtttctga
      661 aatggaatca cttctcaaaa aagaaggatt cgtgaaagtc actgttcaac gactgttcat
      721 ctgggagatg gcgatgtatc aaaggacaag ttaagaccct aaaggtcttt ctccgataac
      781 tgaacaatta attaaaaaag agaattagcg ttttatcgag tctcgattga aataaagacc
      841 agtagagtct ctggattccc tcttgactct gacattgggg gagatgatac agtgggagcg
      901 aaaggagatt cccatgaacc actggcaatt tgaacagtac aaacaaatcg atgaaatcag
      961 tcgtcaaaga atgcaatctg aagctagagc ggaaaattta gttcgcagac tccgcacgta
     1021 tcacccgggc ccattcgagc gcgtaatgtt ccggcttgcc aactggatga tcttgaaagg
     1081 aaagcagctc cgtaggcgct atgaaatccc agaaacgggt tgtagccaac aggccccacg
     1141 caatctaata tcatgattct ctcaactccc tcttgactct gacattgtgg cagggtgtat
     1201 agtattgaaa gttctgacga atgttcaaaa tcggtgaatt ctcccagtta agtcgagtat
     1261 cggtgcggat gctgcgtcac tatgaccagc ttgggctgtt gaagccatct cgcacagaca
     1321 cgtttacgaa ctatcgctat tactcggcgg atcaacttcc gcgtctgaac cgcatccttg
     1381 ctctgcgcga cctgggcttc tcgctggaac agatcggaaa tatgctcgat gaggatttat
     1441 ctgccgaaca attgacgggc atgctcaagc tcaaacgggc agagatcgaa caacagatgc
     1501 aggttgaaca gttacgcatg gcgaggctgg aggcgcgaat tagtcagatg aacggtcaaa
     1561 ctgatcctgg tcaatatgat gtggtggtgc gcgagatcga accccaatta gtggcgacat
     1621 atcgtgaact tgcgcctgac gacgacagga ttcagtatat gtttgaagaa ttggaagcct
     1681 atgttacgca gttcaacgcc cgtgcggata agcctcccat gtcgatttat cacgaccctg
     1741 agtatcgtga agagaatatt gacgccgaag tcgtggttcc gctaaagtcc gcgatccccg
     1801 gcaccgatgt attccacgtg ctcgaaatgg cgcgtatccc caaggctgct tgtgtagttc
     1861 acaccggcga gtacgcgacg atttatcagg cctacaatgc tctgctacgt tggattgaag
     1921 caaacggtta tcgaatgaag ggtccgattc gagaagtgta tctaagctac agcgcggatg
     1981 ggttgggctt tgagcttcca cctacctatc tggtgaaaga atcaaatccg tttgtcactg
     2041 agttgcagtt ggagatagaa aaagactaat gtgtcgttgc gacgaagcaa tctcaaacta
     2101 acatcaaaga acgagattgc cacgctgccg acgaacatcg gcagctcgca atgacgatat
     2161 aacaggagat accatggcat attatttagt aaaagccaaa tatcataagc atttactcgc
     2221 tgaattacgc acacggctcg acagtggcga gatcaacaaa atgcgaccct ttggaaccgc
     2281 gctgcagtac agcctcgata acgcgcggct tgacccaaag ggcgagggtt ggagcgcgtg
     2341 ggaagaagag gattattgca gtccaccgct tgcacaggaa cgcgcggcgg tgctggatac
     2401 atacttcacg tcgctcagcg tagaaaaggt atctcagggg aacggttggt cccgcatcga
     2461 gtctctgccc aggctttgga agaaatccaa cggaggaaca gatgcgctat aaattattgg
     2521 gccgcagtgg cttacgggtt tccgaactgt gcctgggaac gatgacattc ggagatgatt
     2581 ggggctgggg cgctccgaag gaagaaagcc gtaagatgtt cgatgcgttt gcagagtcag
     2641 gcggaaactt cattgatacg gcaaacaact ataccaatgg gaccagtgag aaattcgtcg
     2701 gagaatttat tgcctctgat cgagaccgtt ttgtagtcgc caccaaatac acgttgagcg
     2761 aacgcgccga agaccccaac tacggaggga atcatcgaaa aaatatgttc cgctcgattg
     2821 aaggaagctt gaagcggctg aacacagatc acatagacct gctctggctc catatgtggg
     2881 atggcgtgac gcctatcgaa gaagtcatgc gcgcgttaga tgatctggta cgttcgggaa
     2941 aagtcttgta tgttggaata tccgatactc ctgcctgggt tgtttcgcaa gccaacgcca
     3001 ttgcagaatt acgcggctgg tcacgattta tcgcctacca gggtgagtat agccttgcaa
     3061 gccgcgcacc agaacgggac atcatgccca tgactcaggc gctggaaatg gcgtttacgc
     3121 cgtggggcat tctggagggc ggtgaaatga ccggaaaata taatgcgccg tctgatgagc
     3181 caaaacgatc gagcgatacc agtccacgaa tcaaatccct ggcagctgaa ttgatgtcgc
     3241 ttgctgagga aattggtcgt accccatcgc aaatcgcgat caactgggtg aggcagcagc
     3301 cttatttagt tgtccccatt attggctcgc gcagtgaaaa gcatcttcga gataatatga
     3361 gttgtctcga atttgaattg acacctgatc aaataaaacg cttaagcgca gccagcccga
     3421 tcgacctggg cttcccacat tcattccttg cgtctgatca cgtgcgcggc ctgatctttg
     3481 gtaagacctt tgcacggatt gataatcatc gggtgagagt tgggtaagtt gcaagagtca
     3541 atcattgtga atgcaaacag ctctgtgact tgtcacagag ctgttttttt tcattcatca
     3601 agtcgaatta attcaggttt ggtttttgct tatgccattc agtttcctgt tcataagcgt
     3661 agcctgcatt caacactttt gcatcggccc cggcacgtga aacgatctgc aagccaatgg
     3721 gtaaaccatc tcttgtgaaa ccacagggca gtgacagggc gggcaagcct gtcagattga
     3781 acggtgcggt gaaccgggtg aggcgcgcag cttgttcgag cgcatctgcg ccttccacag
     3841 ccggagcaga atttggagtg gttggaagaa tcaggaagtc atacgcttca aagaattgct
     3901 cacatcttct tcggacctct gtttgagtcc gtctcgcgag gatatattca gtggaagtgg
     3961 tcttcgcccc cgcttccagc ctttgacgga tatcatcacc aaaccaatcg ggatgttcct
     4021 taagccgatc gcgatgtact gccgcgccat cggattgagt cattgtttta tttgcaaggg
     4081 cggcgtcgcg catccaatcg acatttacat cctccaacct gcaacccagg gattcgaaga
     4141 cgcgtctcgc ttccttcacc gcttctaata cttcggagtc cgcgccttcc acatactcgc
     4201 cgaccccgaa agcgattttt ttcccgccta cagcatcggt caggtgactg agataatcac
     4261 cggtcagcag cttaatggat gcagggtccc taggatcgta aatggaaatt acctgtaaca
     4321 gaagcgcggc gtcctttact gtctttgtga gcggacccgc gtgatcgagg ttccaggata
     4381 acggaaatac accgcgcagg ctcacgcgtc cataggacgg tttaaatccc accacaccgc
     4441 ataacgacgc agggatgcgg attgagccgc ctgtatcggt tcccagcgcc cccaatgcca
     4501 tgtctgccgc taccgcaatc gccgacccgc ttgaggagcc accggaaatg cgcgtggtat
     4561 cccatgggtt gcgtgcggtt ccgtaatgcg gattatttcc ggtcacaccc agcgcaatct
     4621 catgcgtgtt cgtttttccg atgatcgttg cacctgcctg cttaagtttt tcgacgacaa
     4681 acgcatcggt ctcgggaata tgttctgaaa agaatcttga accggctgtg gtgcggatcc
     4741 ctgttgtgtc aaagaggtcc tttacagcga tcgggatgcc tcgcagcata ttggttattg
     4801 tgtggctggg tgtggaggcg ctgatcgccg ctgttggttg ctccttaatc acggtaataa
     4861 acgcattcag cattgggttg agtcgttcaa tgcgactcaa acaagcgtcg gcaaaatccc
     4921 gagcggtggc cttttgtgct tgaatgaatt ccaacgcgga ggtgatcgtc agtgagttta
     4981 ggttgtccat gggtctatta taatggggcg ggttaatctt aatgcaatcg gtttcttttt
     5041 caaaaaatag attctggcaa tttttgttta tcctgagtgt gatcgggata atactttgtt
     5101 gggttgcaac atttcgattt ggtgcggggg tagctgcaga ttcgactaaa tatctttccg
     5161 tcgcagagag cttactggca ggcgagggct tgcgtgatca tctggatatg cccctgcttg
     5221 cctggccgcc actctatcca atacttttgg cggcactgag tcgtctcaca ggtttggatg
     5281 tgtttgtcgt tggctggtat ttcaatgttt ttctggctgg attgaacatt tttctcagtg
     5341 gaattatctt ttgtcgcgta ttttcagaaa agcctctcta tgcgtatctg ggaagtctct
     5401 ttgttttgtt ggccagttcg tcacttcgca tgcacgcgac gattagttct gacccattct
     5461 atctgacctt gacactcggg tttctcatcg cagtcgatcg ttatatcaag cgacattcgt
     5521 atagcgcatt tctatggatg atcgtattta gtgtgcttgc tccattgcag cgctatgtag
     5581 gtcccgcagt ttgtgttacc gccgtcctgg tgattctgat tgagaagcgg aaatccatcc
     5641 gtgtactttt acgcgactca atcttgttgg gagtagcttc cattttgccg attgcatggt
     5701 ggttacttat ccataacatt atgacgtatg gctctttatg ggggtcaacg ggtgatcgca
     5761 ctgtggatgt tcttaaaaac acagaactgg cactgaccaa gatgctgcac tggtttgttc
     5821 cctatctatc attcctcatg ccgatcctaa acagaccgtt gatcctactg agtgttatgg
     5881 cactcgttat tttcttgctc aaccgaaaaa acaaaggcag cggacgcctc tggataaact
     5941 tactggcggc tccctccgcg tacccaagct tgatctacgc cgtggtttat tttgcggcag
     6001 tagcagtgac aatcgtgact attgttcacc gggacctgtt ttctgatcgt tattatgtga
     6061 tgttgctggt tcctacggtg attttccttt tcattacgtt tgatacactc atccgaccgc
     6121 ttttagcagc ttcctacaca cgatttagtt atattctgat tcttattttt gcattgtggt
     6181 ctgtttatcc gatttatacg atttcggagt acctggcaaa tgcaagcacg cagggtgagc
     6241 caagcgggtt taacatgtat aacaatcgta cctatcgcga catggacctg gtaggggaaa
     6301 tgcagcgatt aagagaagac cagccggctg cgattttcta cagtaactac gtggatgccg
     6361 tttggtttta tacgggtaag ccggttgctc tactgccgtt agcaaatgtt tcaagcccga
     6421 gcgaggcata cgctggttgg ccaggggaca agcctggtta tatcgtctgg tttgagccga
     6481 acgaatataa gcattacctt tcgccagaaa agatcgcgga atttgcgtct gtgcaattgg
     6541 tctttgaggg agaaggcggg aagctctatt acgttcagcc tcgttgatcg gcacaagctt
     6601 ttgtctgaaa agtgatttaa cgtttgagca cagccagtat cgatgtgccc cacggaaaat
     6661 tcacggcgtt gatcatagac gcttcgaacg aaaatagctt tgtgagcaat ccgttgattg
     6721 gctgtgaagg gacgcgatgt gtttgcacca gatgagcttt aagttgttct ggcgttgcca
     6781 gcttaggtgg acgaaatagc atgcgactca gaaatagagc ggggctgaga aagaacataa
     6841 agtaatttgt tcgtagaagt tcgaaattcg tttgttcagc taggatttga aaatcctgcc
     6901 tggaatagcg acgttgatgg agtgcgaggt cgtcgttata ggtccagaag aagtttagcg
     6961 cgggcgtcgt cacaaagagc agtccgccag gtcgtaggct cctgtagatc tgtctgagga
     7021 cttcctcatg atctgggatg tgttccagta cgtccagaag aaaaacaaca tcccaggttt
     7081 ctgcccaggc aaggttgagg aggtcaattt ggtatcttgt tgcgaagccg cctacgacgg
     7141 gttctgccaa tgagagggca tgcatcgagc tgtcacccag ggcgattttg tggaacatgc
     7201 ccgggcggtg cgtgtgtata tattccagcc aaccgccaca accaccgccc atatcaattg
     7261 cacgcaagcc tgttctttga ccgagatgtt ttggaatctc tttttccaac gcatggagaa
     7321 gcagtttatg acgaccgcga taccaaaaat gttcgcgctg cattcgaatg agcacttcga
     7381 aacccatctc atcgtattca tcatttcgat gtttgggacg cacacgcggg acaaaaattc
     7441 cttgttcttc aatgaaatcc acctgatgaa tcatgttgag gaaacctttt gtattaaatt
     7501 agacttggaa caaccctttc aagtacttgg acatccgtag aaagtcagga tgtccaagca
     7561 acatgttttg tcaactcaaa tttctgtttg acgctcgcaa atttagaata acccaacgac
     7621 ctttcctgtt ttaagatcaa gatcgatatc gtaataagag gggcgggttg gcaagcctgg
     7681 catcgtccgc atctcaccca ataatggata caagaatccc gcgccgacgc tagcgcgtat
     7741 atcgcggatc aacacacgga agccggt
//
LOCUS       DASTJP010000254         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_453889, whole genome shotgun sequence.
ACCESSION   DASTJP010000254 DASTJP010000000
VERSION     DASTJP010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453889"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1242
                     /locus_tag="VFQ23_07750"
     CDS             37..1242
                     /locus_tag="VFQ23_07750"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9906518.1"
                     /translation="MNALDTLVELSGQMELEHAEEKRGGLDTSRENPAGHSTTGEPAK
                     PACFTPKEKRAAFVHPAQLPNGKQVHLLKTLLTSACERDCYYCPFRAGRDFRRATFKP
                     QEFAEMFMKLNQANMAQGIFLSSGIAAGGANTQNRILDTAEILRKKFGFRGYVHLKIM
                     PGAEKGQVERLMQLADRVSINLEAPNKERLTRLAPHKNFYEELFQPLKWVEEIRRTQP
                     AQKFWNGRYPSTVTQFVAGGSDETDLELLTTTDWLTKNVRLQRAYYSAFHPIRDTPLE
                     NKAAVDPLREHRLYQASFLLRDYGFDLEEMPFLQNGNLPLPTDPKLAWAEHNLKEKPL
                     EINTAERRELLRIPGIGPKHADAILQVRRSAKLRDLTALRKLGIVVARVAPFILLDGK
                     HTEQQLPLF"
     gene            1275..1838
                     /locus_tag="VFQ23_07755"
     CDS             1275..1838
                     /locus_tag="VFQ23_07755"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9906519.1"
                     /translation="MENNIPLANIFDGWNGYQTSLVNAIAPLTREQLRWQPAASSRSI
                     GETARHISLGRVSWFSRMDAPGSQELVDRIEQWETDRDGNRDVVESSIAITDNADELV
                     HWLEATWQMIHKTLATWTIADLAQTYRHTWNGQTYAVSRQWTIWRILSHDIHHGGELS
                     LLLGLQGIEAFELSDLFGHIILPPLAD"
     gene            complement(1850..>2090)
                     /locus_tag="VFQ23_07760"
     CDS             complement(1850..>2090)
                     /locus_tag="VFQ23_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906520.1"
                     /translation="STLLFESESEPLSHGPLTDRESEVLGLLSKGLANKQIAVALGIS
                     EHTVKFHVSSIYTKLNVTNRTEAVREGLRGGWIAL"
BASE COUNT          541 a          531 c          530 g          488 t
ORIGIN      
        1 tcttgacaag tagaatatct gttctataat caatttatga acgcgctgga taccctcgtt
       61 gaactttcgg ggcaaatgga gctggaacat gcggaggaga agcgaggtgg tctcgataca
      121 tcccgcgaaa accctgcggg acactcgacc accggtgaac cagccaagcc tgcctgtttc
      181 acgccgaaag aaaagcgcgc ggcgttcgtt cacccagcac aactccccaa cggcaaacaa
      241 gttcatttgc taaaaaccct gctcacatct gcctgtgagc gggattgtta ttactgcccg
      301 ttccgtgcgg gacgtgactt cagacgcgcc acattcaagc cgcaggaatt tgccgagatg
      361 ttcatgaagt taaatcaggc gaacatggcg caaggtatct tcctcagctc aggcattgca
      421 gccggtggtg cgaacacaca gaatagaata ttggatactg ctgaaatcct gcgcaagaag
      481 tttggctttc gcggttatgt gcatctcaag atcatgccgg gggcggaaaa aggacaagtg
      541 gagcggctga tgcagctggc tgaccgcgtc tcgatcaatt tggaggcgcc gaacaaagaa
      601 cggttgacaa gactagcgcc acataagaat ttttatgagg agctctttca accattgaag
      661 tgggtggaag agattcgacg cacacagcct gcgcagaaat tctggaatgg gcgttatcca
      721 tccacagtta cacaatttgt ggcgggcggc tccgatgaaa ctgatctgga gttgttgacg
      781 accacggact ggctgacgaa aaacgttcga ctccaacgcg cctattattc tgcctttcat
      841 ccgattcggg atacacccct tgaaaacaaa gccgcggtcg atccattgcg cgagcatcgg
      901 ttgtatcagg catcgttttt attgcgagat tacggatttg atctggaaga aatgccattt
      961 ttacagaacg gcaatctgcc cctgcctact gatcccaaat tggcctgggc agaacataat
     1021 ctaaaagaaa aacctctgga gatcaacaca gcggaacgac gtgaattact tcgcattcct
     1081 ggcattggac cgaaacatgc cgatgcgatt ctccaggtaa ggcgaagtgc aaaactgcgc
     1141 gatttgacag cactgcgcaa attaggtatc gtagtggcac gcgtcgcacc gtttatatta
     1201 ttggatggaa aacataccga acagcaattg ccgttatttt gatatcacga gcaggatgga
     1261 gctacgaaac cggcatggaa aataatatcc ctcttgcaaa cattttcgat ggatggaatg
     1321 gctatcagac cagcctggta aacgcgattg ctcctctgac acgtgaacag ctgcgatggc
     1381 agcctgcagc ctcctcgcgc tcgatcggtg agaccgcgcg ccatatcagt cttgggcgag
     1441 tttcgtggtt ctcgcgtatg gatgctccgg gtagccagga actcgtcgat cggatcgagc
     1501 aatgggaaac tgaccgggat ggcaaccggg atgttgtaga gagttcgatc gccatcacag
     1561 acaatgcaga tgaactggtc cattggctgg aagccacctg gcagatgatc cataagactc
     1621 tcgcaacatg gactatcgct gacctcgcgc agacctaccg gcatacctgg aacggacaaa
     1681 catatgcggt ctcgcggcag tggacgatct ggcgcatcct atcccatgac attcatcatg
     1741 gaggggaatt gtcgctgttg cttggtttac aggggattga agcctttgaa cttagcgatt
     1801 tattcgggca tattatcttg ccacccctcg ccgactaaca acattcttat tacagtgcga
     1861 tccaaccccc tcgcaatcct tcacggaccg cctctgttcg attggttaca ttcaacttgg
     1921 tgtagatcga agagacatga aacttaaccg tatgttcgct gattcccaac gcaaccgcga
     1981 tctgtttgtt tgccagcccc tttgataaca accctaaaac ttcagattct ctatcggtaa
     2041 gcggcccatg tgacagtggc tcgctttctg attcaaacaa cagcgtggat
//
LOCUS       DASTJP010000255         6548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_857574, whole genome shotgun sequence.
ACCESSION   DASTJP010000255 DASTJP010000000
VERSION     DASTJP010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857574"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..2122
                     /locus_tag="VFQ23_07765"
     CDS             443..2122
                     /locus_tag="VFQ23_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HET9906521.1"
                     /translation="MKILILGGYGTFGGRLAYLLAEDEKLNLFIAGRSIQKAKNFCET
                     LPSGAQKVPVFFDRNANVEAQIREIKPDIVVDAMGPFQIYGDDPYDVVKACLANAVDY
                     MDLADGSDFVKGISQFDEEAKAKNIYILSGVSSFPVLTAAVVRKLSHDMTRVNTIKAG
                     IAPSPYAGVGLNVIRAIASYAGKPLSLIRNGKPDTGYALTETMRYTICPPGHLPLKNK
                     LFSLVDVPDLKVLPDLWRELDSIWLGAGPVPEILHRMLIGLAWLVRLRILPSLLPFAS
                     LFHFVINILRWGEHRGGMFVSVEGTGRGGERIERSWHLLAEGGDGPLIPSMAIQALIL
                     HSLDGKKPSSGARPATQELEVSDYDAIFKSRTIYTGQRESRSDSDSAPLYKRLLGEAW
                     DMLPAPLTELHNITSGEHKAVGFARVETGNNLFARLIAVLYNFPQTGDDVPVRVTFRV
                     RDNGEIWERDFAGIKFSTFQAEGSGYADKLLVEKFGPVTFWMALVLKQQEFHLVTRAW
                     SIFGIPLPLAFAPNASVYEYADGNDFCFHVEVTHWLMGLLVRYQGRLAPVK"
     gene            2188..2784
                     /locus_tag="VFQ23_07770"
     CDS             2188..2784
                     /locus_tag="VFQ23_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906522.1"
                     /translation="MYKQNFTLANLIFAAILVGCAPLAGEAKTLEDYRMPTDVPSADS
                     LTQTAQTEVSSLIPPKSCPVTTSENISFKAPEPFSPTAPWLGNFWYGSEHLWTALQND
                     GVWAGLPANSDGFSQKIMWWSSLYSLKDELEPALVVSGRRLDGEADPLRFYGATNAMA
                     DDIGEAMLTGVEIPTLGCWEITGQYKKGQIQFVVWVAQ"
     gene            complement(2828..3571)
                     /locus_tag="VFQ23_07775"
     CDS             complement(2828..3571)
                     /locus_tag="VFQ23_07775"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906523.1"
                     /translation="MPPVLTIRNLSAVFPDNNGGLRALDGLSFDVHPREFVCVLGPSG
                     SGKTTLLRILAGLIQPTSGSVTFGHVWKQPRIGMVFQQANLMPWRTVMDNIKLPLEVN
                     HVDEPTARLKAQEMIELVGLQGFEDSLPRDLSGGMAQRVAIARALIHDPDLLLLDEPF
                     ASLDAMTRERMWMELSRIWHARQKTVIMVTHSINESLFLADRVLVLTQRPGKVKLDLQ
                     VDLPRPRTDDIRYTPQFGSLARKLRDAIE"
     gene            complement(3722..4696)
                     /locus_tag="VFQ23_07780"
     CDS             complement(3722..4696)
                     /locus_tag="VFQ23_07780"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9906524.1"
                     /translation="MFRKFILITFVFALALSACGGSGAQNEQLTHIRLPMGYIANIQY
                     APFYVAAEKGYFREAGIELEFDYKFETDGVALVGAGELPFAVVSGEQVLLARSQGLPV
                     TYVTAWYQQFPVSVIARSDQGVLIPQDLKGKKVGLPGLFGANYVGLRALLFEAEMSES
                     DLTLDAIGFNQVDLMAVGQHDIIVGYAANEPIQLQAMGIAVTEIRVADYVQLASNGIL
                     ASEKVISENPELVRAFVGAFIKGLSDTIANSDEAFSISEAYIPNFTDLDAEVQKKVLE
                     TSIEQWKADRLGHSDPQAWENMQNVLLEMDLIPEKMDLNKAFTNEFIP"
     gene            complement(4813..5568)
                     /locus_tag="VFQ23_07785"
     CDS             complement(4813..5568)
                     /locus_tag="VFQ23_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906525.1"
                     /translation="MTKRFIPIIIPILTFLLAWHLITRLSGIPNFILPSPLSVWTRFL
                     RAVSDGSLPFHTGITLIEIVLGLLAGVSFATVVGYLLAKSRSLERVLSPYLVASQAIP
                     IVAIAPLLVIWLGEGILSKVVICALIVFFPVLVNTIVGVRAVPTALYDLMNSLHATRA
                     QILLKVEVPASLPVFLGGLRIGATLSVIGAIVGELVDAEEGLGFLLQLGDFQYDTPMV
                     FVAVFMLIALALMLYGIVTMLERRFLKWQDTSR"
     regulatory      complement(5852..6013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6038..>6548)
                     /locus_tag="VFQ23_07790"
     CDS             complement(6038..>6548)
                     /locus_tag="VFQ23_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HET9906526.1"
                     /translation="EFHMAINKVEKSLIRTEADEVTYNLHVMLRFDFELQLLEGSLSI
                     RDLPEAWHERFEQDFGIVPPNDSDGVLQDVHWYAGVIGGSFQGYTLGNILSAQFYDAA
                     LRAHPEIPDEMKQGKFDTLHGWLRENIYRHGSKFTAPEIIERASGTPLTIEPYINYLK
                     TKYGALYAL"
BASE COUNT         1674 a         1651 c         1650 g         1573 t
ORIGIN      
        1 tgttgctctc aactggatcg gtttttacgt aagcgcgcag tatcgggata accacggaaa
       61 tctatcgcct gtctactgcg acgatattag ggtggaaggg tttccgtcct cgtcaacaaa
      121 tacttctaca ccgtaaaagt aaaacaaatc tgatttcact cgggagcagt tcacaacatg
      181 ctagaacgtg tctcaaaaac actctgtcac tctgagggag cgctgttggc gaccgaagag
      241 tctcgtactt tcgttgttgg agatccttcg ctccgctcag gatgacattt ttgagatgat
      301 ttgtagtcta taaggtgtga ctgcaaaatg agccctgcct acccacaaga attagggctt
      361 tatgcggcgt agctcacttt ttttaagata aaatccgtga agcggtgatg gagcatataa
      421 caaacgtaag cagatcccaa atatgaaaat cctcattctg ggcggttatg ggacgttcgg
      481 cggcaggctt gcctatctgc ttgccgagga tgaaaaattg aacttgttca ttgcggggag
      541 atccattcaa aaggcaaaaa atttctgcga aaccttaccc tccggcgcac aaaaagtgcc
      601 cgtatttttt gatcgcaatg ccaatgtaga agcccagata cgggaaatca aaccagatat
      661 tgtcgtggac gcaatgggtc catttcaaat ctatggggac gatccctatg acgtggtgaa
      721 agcctgtctt gcgaatgcgg tggactatat ggatctggcc gatgggtctg attttgtaaa
      781 agggatcagt caatttgatg aggaagccaa agcgaaaaat atttacattc tttcaggtgt
      841 gagcagtttt cctgttctca ctgccgcagt ggtgagaaaa ctttcgcacg atatgactcg
      901 agtgaacacc atcaaagctg gtatcgcgcc ttctccgtat gcaggcgtgg gcttgaatgt
      961 gatccgtgcg attgcctcgt acgcggggaa gcctctatcg ttgatccgaa atggaaaacc
     1021 ggacacaggc tatgcgttga ccgaaacgat gcgctataca atttgcccac ccggccattt
     1081 gccgcttaag aataaattgt tctccctcgt cgatgtccca gacttgaaag tgctccccga
     1141 cctatggcgc gaacttgact cgatctggct gggcgcggga cccgtcccgg agattcttca
     1201 cagaatgtta atcgggctgg cgtggctggt gcgattgaga attctgccgt cactgctgcc
     1261 atttgcatcg ctttttcatt ttgtgatcaa catcctgcgc tggggtgaac atcgaggcgg
     1321 gatgttcgtt tcagtcgaag gaacaggccg aggcggggaa agaatcgagc gatcctggca
     1381 cttactggca gaaggcggtg atggcccgct catcccctcc atggcaatcc aggcgctgat
     1441 ccttcacagc ctggatggga aaaagccatc ctctggtgct cgacccgcga cccaggagct
     1501 ggaagtctca gactatgatg cgattttcaa aagccggacg atttacacag gtcaacgcga
     1561 atcgagatcg gattcagact ccgctcctct gtacaagcgg cttcttggcg aggcatggga
     1621 catgttgccc gctcctttaa cagaactaca caacatcaca tctggagaac acaaagctgt
     1681 aggatttgcg cgcgtcgaaa caggaaataa tttattcgcg cgtttgatcg cagtgttata
     1741 caattttccg caaacgggcg atgatgtacc tgttagagtt acatttcgtg ttagagataa
     1801 cggggaaatt tgggaaagag attttgcagg cataaaattt tcaacatttc aggcggaggg
     1861 gagcggatac gcggataagc tcctggtgga aaagtttggt ccggtaacgt tttggatggc
     1921 tctggtcctg aaacaacagg aatttcattt ggtcacacgc gcctggagca tctttggcat
     1981 cccattgccc cttgcattcg ctccaaacgc atctgtgtat gaatatgccg atgggaatga
     2041 cttttgcttt cacgtggaag taacacactg gctaatgggc ttgcttgttc gctatcaggg
     2101 caggctggct ccagtaaaat aatctgtcag actgatggaa ccctccttcg ctttgaggtg
     2161 tattcagggt aaagcaagga gatacggatg tacaaacaga atttcacgtt agcaaacctc
     2221 atttttgcag ccattttggt aggttgcgcc cctctcgccg gagaagccaa aaccctcgaa
     2281 gactaccgga tgccaaccga tgtgcccagt gcggactcgc tgacccaaac ggcacaaacg
     2341 gaagtttcat ctttgatccc tccgaagagt tgccctgtaa caacctcaga aaatatatct
     2401 ttcaaagccc cagaaccatt ttcacccact gcaccctggt tgggcaactt ctggtatggc
     2461 tctgagcatc tatggactgc acttcaaaat gatggcgtct gggcgggctt acccgcgaac
     2521 tcggatggct ttagtcagaa gatcatgtgg tggagcagtc tgtactcgct aaaagatgag
     2581 cttgagcctg ccctggttgt gtctggacga aggctggatg gagaagcaga cccgctccgt
     2641 ttttatggag cgaccaacgc gatggcagac gatatcggcg aagccatgct cacaggcgtt
     2701 gagattccca ctcttggatg ttgggagatc accggtcagt ataagaaggg tcaaattcaa
     2761 ttcgtagtct gggtcgcaca gtaagataaa cagacttccg aaggcttgga gccttcggaa
     2821 gtctttctta ttcgatggcg tctcttaact tccgcgccag gctaccaaat tggggtgtat
     2881 agcggatatc gtcagtacgc gggcggggca aatctacctg caaatcaagt tttactttac
     2941 cgggtcgctg ggtcaatacc agaacacgat ctgccaaaaa caacgactca ttgatcgaat
     3001 gcgtcaccat aatgaccgtc ttttgacgtg cgtgccagat gcgcgaaagt tccatccaca
     3061 tgcgttcgcg ggtcatggca tccaaggaag caaaaggctc atcgagcagg agcaggtcag
     3121 ggtcatggat gagcgcacgc gcaatcgcca cacgctgtgc catcccgcct gacaggtcgc
     3181 gcggcagtga gtcttcaaat ccctgcagac caaccagttc gatcatttcc tgggctttta
     3241 ggcgcgcagt tggctcatcg acatgattta cttcaagtgg aagtttgatg ttatccatca
     3301 ccgtgcgcca cggcatcagg ttcgcctgct gaaagaccat gccaattctt ggctgtttcc
     3361 agacatgacc gaacgttacc gaacccgatg tgggttgaat caatcccgca agaatgcgca
     3421 acagagtcgt cttgcctgaa cccgagggac ccaacacgca aacgaattcg cgcggatgaa
     3481 catcaaagga tagtccatcc agggcgcgca aaccaccgtt gttatcagga aacacggcac
     3541 tcaggttgcg aatggtgagg acgggaggca tagctatcta tttgtcattg cgaggagcgt
     3601 tcgttgcgac gaagcaacac ctgcacttgc gccaggtgca agtgtctcat gctaagttgg
     3661 agattgcttc gccctacacc tgcactggcg tgccggcgca agtgtcgggc tcgcaatgac
     3721 attatggaat aaactcgttt gtgaacgcct tattcaaatc catcttctca gggatcaaat
     3781 ccatctcgag caaaacattt tgcatgttct cccaggcttg cgggtcagaa tggccgaggc
     3841 ggtcagcttt ccattgttcg atggatgtct caagcacttt tttctgcacc tcggcatcga
     3901 ggtcagtgaa gtttggaata tacgcttcac tgattgaaaa ggcttcatca gagttggcga
     3961 tcgtatctga aagtcctttg atgaacgcgc cgacaaaagc gcgcaccagt tcagggtttt
     4021 ctgagattac cttttcactt gccagaatcc cgttcgacgc cagttgcaca taatctgcca
     4081 cacggatttc ggtcacagcg attcccatcg cctgtaattg gatcggttca ttagctgcgt
     4141 agcccacgat aatatcgtgt tgaccgactg ccatcaggtc cacttgattg aaaccaattg
     4201 catccagagt cagatcgctt tcgctcattt cagcttcaaa caacaaggca cgtaagccga
     4261 cataattcgc gccgaataaa ccaggcaagc caaccttctt gcctttcaaa tcctgcggga
     4321 taaggacgcc ctggtcgctt ctggcaatga cagagacggg gaattgttga taccaggcag
     4381 tgacatacgt caccggcagt ccctgtgaac gggcaagcaa aacctgctcg cctgaaacaa
     4441 ccgcaaacgg aagttcgccc gctcccacaa gcgcaacacc atcggtctcg aatttgtaat
     4501 caaactccag ctcgatacct gcctcgcgga aatatccttt ctcagcagcg acgtaaaacg
     4561 gtgcatattg aatatttgca atatatccca tcggcaagcg gatgtgagtt aactgctcat
     4621 tctgtgcccc ggaaccgccg caggctgaga gagcaagtgc aaaaacaaaa gtgatcaaaa
     4681 taaatttgcg aaacatagaa tctccttgta tttatcctcc gcgctgagcg gagccgtagg
     4741 cgcagtcgaa gcgacgattt tatgtgagac gtgcgcttcg tctgcatttc tcgcgttcgc
     4801 tcgatactcc gctcagcgcg atgtatcctg ccatttcaaa aatcttcttt ccagcattgt
     4861 cacaattcca tacaacatca acgccagggc gatcaacata aacactgcga caaacaccat
     4921 cggcgtgtca tattgaaaat ctcccaattg cagcaggaat ccaagccctt cttcggcgtc
     4981 caccagctca ccgacgatcg cgccgatgac agacaacgtc gcgccaatgc gcaagcctcc
     5041 cagaaaaacc ggcagggatg ccggcacttc cacttttaat aatatttgag cgcgcgtggc
     5101 atggagtgag ttcatcaaat cgtatagcgc ggtgggaacc gcccgtacac caacaatcgt
     5161 gtttaccaat acagggaaga acacaatcaa ggcacagata acgacttttg aaagaattcc
     5221 ttcacccagc cagatgacga gcagaggcgc aatcgctaca atcggaatcg cctggctggc
     5281 aacgaggtac ggagatagca ctctttccag cgatcgcgac tttgcaagaa gatatccgac
     5341 tactgtggcg aacgacaccc cggccagaag tccaagtacg atttcgatca aggtgatccc
     5401 ggtgtgaaag ggcaggcttc catcactgac tgctctcagg aatcgagtcc acacgctgag
     5461 tggagagggc agaatgaagt tcggaatccc gctcaggcgc gtgatgaggt gccacgcaag
     5521 caggaaagtc aatatgggga taatgatcgg gatgaatcgt ttagtcataa tcgttgattc
     5581 gtcacacctg cactccccgg caccgaccaa ccggggcggt gtgcgtgccg gtgcaagtgt
     5641 tgcgaggagg gctttgccca acgaagcaat cttttcgtca aacaggagat tgcttcgggc
     5701 tgttgccctc gcaatgacag aaaaacaaaa gcgcctcgca tagaatgaat tgcggggcgc
     5761 ggaatcaagc taaggctgag ttagagccga aaaaaatccc gaaaataaat gacgttttcg
     5821 ggatcgatgt cgaatcagac tcgcgtgagc ctatctcctt cttttatcca gactataact
     5881 gtcggctctg gaattacacc agatcatgct catagaacga ctgaagtcat tactacattg
     5941 ctcgtgggct ataccaccga tcgggaattc acatacacta ttgtgcatat gtcaccctgc
     6001 cccgaaggtt attatttagt tgaacccatt ataacattta cagcgcgtac agcgcgccat
     6061 atttggtctt gaggtagttg atgtacggct caatcgtaag cggagttccg ctggcgcgtt
     6121 caatgatctc aggtgcggtg aacttgctcc cgtgacgata aatattctca cgaagccagc
     6181 catgtaaggt gtcaaacttg ccttgtttca tttcgtccgg aatttcaggg tgggcacgga
     6241 gcgcggcatc ataaaactga gcagagagga tattgcccag tgtgtatccc tggaatgatc
     6301 ctccgatgac accggcatac cagtgaacat cctgtaacac tccatcacta tcattgggcg
     6361 gcacaatgcc aaagtcctgt tcgaagcgct cgtgccaggc ttccggtaaa tcgcggatcg
     6421 agagactccc ttcaagcaat tgcagttcaa agtcgaagcg cagcatgacg tgtaagttat
     6481 aggtcacttc atctgcctcc gtgcggatga gtgatttttc gactttgttg attgccatat
     6541 ggaactca
//
LOCUS       DASTJP010000256         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1182687, whole genome shotgun sequence.
ACCESSION   DASTJP010000256 DASTJP010000000
VERSION     DASTJP010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182687"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFQ23_07795"
     CDS             <1..450
                     /locus_tag="VFQ23_07795"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HET9906527.1"
                     /translation="TYKAYIPTPPTFAVYQKTIEDFFSDVPYVAKCLLRNELFPVKWA
                     LDYDMKHLFLRRMLEWRAELDHDWSMPVGALGKGLKKRLPKEIWSQLEETYVGSDIAE
                     NWEALFKTIRLFRQVAMEVGEALGYAYPQALDERVTAFAKEMQTTSG"
     gene            607..1011
                     /locus_tag="VFQ23_07800"
     CDS             607..1011
                     /locus_tag="VFQ23_07800"
                     /inference="COORDINATES: protein motif:HMM:NF025879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HET9906528.1"
                     /translation="MGCHVEQAAGNFSGEIKDGFQLVKDWAAQKGFDLSPLKVIGIPQ
                     VSNGQLTSYECCIELPEPVDVNESGIQTKQLTGGKYAVLTLEKDSATIGETIGRFFAE
                     YVPEHDLVIDNDRPGYEIYYEHIMEFCVPVRE"
     gene            1219..2604
                     /locus_tag="VFQ23_07805"
     CDS             1219..2604
                     /locus_tag="VFQ23_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HET9906529.1"
                     /translation="MTNILCIGAGYVGGSTIPIIASHCPDHRFVIVDINEKRIEAWQS
                     DQLPIYEPGLDEIVRQTRGKNLFFSTDVDRHISEADIIFVSVNTPTKMFGKGAGKAAD
                     LQYWEKTARQILENSTSSKIVVEKSTLPVRTAEAMERILNSNGKGLHFDVLSNPEFLA
                     EGTAVRDMQNPDRVLIGGRETESGQKAVEKLVELYAQWVPRERILTTNLWSSELSKLV
                     ANAFLAQRISSINAISALCEKTEADVNEVAHAIGQDARIGRYFLRASVGFGGSCFKKD
                     ILNLVYLCEHYGLPEVARYWEQVVTMNEYQEARFAANMVASMFNTIAGKRIALFGAAF
                     KANTNDTRESPSLEVCRELLEEQANVIITDPHALENAAYDLGALAQRVTFEPDPYVAA
                     NKAHAIAILTEWKQYTELDYERIYNSMVKPAFIFDGRNLLDHQKLYEIGFNVYPIGKP
                     PLTHLLSYNQS"
     gene            2640..4127
                     /locus_tag="VFQ23_07810"
     CDS             2640..4127
                     /locus_tag="VFQ23_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET9906530.1"
                     /translation="MSRNRVYLVTAGIMLSLFLASMESTVVATAMPTIVGQLGGLEHY
                     SWVFSAFMLASTTTVPLYGKLSDIYGRRKLYVFAMALFLIGSVWCGMANSMTQLILAR
                     ALQGIGAGGIMPLAFILIGEMFSLEQRTKMQGVFSGVWGVSSIAGPLLGGFIVDQLSW
                     HWIFYINIVPGLLAAALVAFAWRDQVHTHERPAVDYAGAALLTLSTVFLLLGLMELGT
                     SRGWVFITGSIVFFVVLLFLERRVADPILPIPLFRDSLFSTAVAHGLLTGWAVFGTIS
                     FIPLFVQSVLGANATQAGITITPMLLGWVSGSIVGTRILLRVGYRKLSLIGTGVFAIG
                     AFLLMVAGVSSSQLLIMAFVTMMGIGMGLSIPGYVIAVQTTVERRHLGVATSMLQFSR
                     SMGGTLGVSVMGAALSARLASNLSASGLDPQMVEQLLEPTPGAEVVINAAARAAMADA
                     IHLIFVIAFVAAALGLVSAFFTPRKELTDTRSSTRESIPSPVSAD"
     gene            4139..>4510
                     /locus_tag="VFQ23_07815"
     CDS             4139..>4510
                     /locus_tag="VFQ23_07815"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HET9906531.1"
                     /translation="MNNQQINEAFERLSTPLIADACLRLEIPIRLAPTGIRPISIGYH
                     IAGRVLPVRHYGSVDIFLEVMGNSQPGDILVIDNDGRVDEGCIGDLTALEAQATGLAG
                     IIVWGVHRDTSELIEIDFPIFS"
BASE COUNT         1044 a         1083 c         1202 g         1181 t
ORIGIN      
        1 acctacaaag cctacatccc aaccccgcca acatttgcgg tgtatcaaaa aacaattgag
       61 gacttcttta gtgatgtgcc gtatgtcgcg aaatgtcttt tgcgcaatga attattcccg
      121 gtcaaatggg cgcttgatta tgatatgaaa catttgtttc tacgccgcat gctggagtgg
      181 cgtgcagagc ttgatcatga ctggtccatg ccagtgggcg cgttaggtaa agggttgaaa
      241 aaacgactgc cgaaagagat ctggtcacag cttgaggaga cgtatgttgg ttcagatatc
      301 gcggaaaact gggaagcatt attcaaaacg atcagactct ttcgtcaggt cgcgatggag
      361 gttggggaag cgctgggcta tgcctatccg caagcgttgg atgagcgcgt tacggccttt
      421 gcaaaagaga tgcaaaccac aagtgggtaa atcattcccc tcttgacgat cctgatatac
      481 tgattccggg tacctaatag ttttagaatt ctatcggtta cgtttgagga gacgccaatg
      541 agcaatctgg cagcgcaagc tttatctgta ggtattcggg atttacttcc ggtagaggta
      601 gcctagatgg gctgccacgt cgaacaggca gcaggcaatt tcagcggtga gataaaggac
      661 gggtttcagc tcgtaaaaga ttgggcagcc cagaaagggt tcgatttatc tcctctcaag
      721 gtcatcggca ttccgcaggt cagcaatggt caattgacca gctatgaatg ctgcatagag
      781 cttcctgagc ccgtggatgt gaatgagtct ggaatccaga ccaaacaact taccggtggc
      841 aaatacgcgg tattaacgct cgaaaaggac tcggcgacca tcggcgagac gattggtcgt
      901 tttttcgcgg aatatgtgcc agagcatgat ctggtgattg ataacgatcg tccgggttat
      961 gaaatatatt atgagcacat tatggaattc tgtgtgcctg ttcgcgaata gccttcaagc
     1021 caagaatacg aggtcagcct tcctcaatct ttgttttatt ttagggtatg attgtttagg
     1081 ctgcgattgt ctgaggagta tctccattgc gtaacgaaaa gaaatccgga cacggtgccg
     1141 tgtttctctc gttacagctg caatcatgtt cagcctgttt tttaacttca atgaatccac
     1201 aaaaagagga ctaatcttat gacaaacatt ctttgcattg gagcaggata cgttggcgga
     1261 tcgaccatac cgattattgc gagtcattgt cctgaccatc gtttcgttat cgttgacatc
     1321 aatgagaagc gtattgaggc ctggcaatct gatcaacttc ccatttatga accggggctg
     1381 gacgaaatcg tccgtcaaac acgtggaaaa aacctcttct tttccacaga cgtcgaccgt
     1441 catatctcgg aggcagatat catttttgtg agtgtgaaca cacccaccaa gatgttcggt
     1501 aagggcgcgg gcaaagccgc ggatctccaa tactgggaaa agacggcgcg tcagatcctg
     1561 gaaaactcca cctcttccaa aattgtcgtg gagaaaagca ccttgccggt tcgaaccgca
     1621 gaagcgatgg agcgaatcct taactccaac gggaaagggc tccatttcga cgtgttatcc
     1681 aacccggaat ttttggcaga aggcacggcc gtgcgcgata tgcagaatcc tgaccgggtg
     1741 ctgatcggcg gccgggaaac agaatcgggc caaaaggcgg tagaaaagct ggtggagtta
     1801 tacgcgcaat gggttccacg cgagcgcatc cttaccacga atctatggtc gagtgaatta
     1861 tccaagctgg ttgccaatgc atttcttgcg cagcgcatct cttccatcaa tgccatctca
     1921 gcactgtgcg agaaaacaga agcggacgta aatgaagtgg cacacgccat tgggcaggat
     1981 gcgcgcattg gacgatactt cctgcgggcg agtgtggggt ttggcggttc atgcttcaag
     2041 aaggatattt tgaacctggt ctatctttgc gagcactatg gtttgcctga agttgcccgc
     2101 tattgggaac aggtcgtcac gatgaatgaa tatcaggaag cgcgctttgc cgccaatatg
     2161 gtggcatcca tgttcaatac catcgcgggc aaacgcattg cgctgtttgg agcagctttt
     2221 aaggccaaca ccaatgacac gcgcgaatcg ccttcgcttg aggtatgccg cgagttattg
     2281 gaagaacaag ccaatgtgat tattaccgat ccgcatgcat tggagaatgc ggcctatgac
     2341 cttggagcgt tagcccaacg cgtcacattt gaacctgacc cttacgttgc cgcaaataag
     2401 gcacatgcaa ttgctatcct tacagaatgg aagcaatata ccgaattgga ttatgaacga
     2461 atttacaact cgatggtcaa acctgccttt atctttgatg ggcgaaacct tcttgatcat
     2521 cagaaacttt acgagatagg cttcaatgtg tatcctattg gcaagccgcc tctcactcat
     2581 ctgttgtcct ataatcagtc ataacctcaa tttgatattc acaagctaga aaggaaataa
     2641 tgtcacgtaa ccgtgtttat cttgttacgg ctgggatcat gctcagcctg tttttggcat
     2701 cgatggaatc caccgtggtg gccaccgcca tgcctacgat tgttgggcag ttgggcggac
     2761 tcgaacacta ctcgtgggtt ttttcagcgt tcatgctggc gtctacaact actgtcccac
     2821 tttatggaaa actttctgat atctatggca gacgcaagtt atacgtcttt gccatggcgc
     2881 tctttctgat cggctcggtg tggtgcggta tggcaaatag catgacgcag cttatcctcg
     2941 cacgggcgct gcaagggatt ggcgcgggtg gcattatgcc cctggctttt atcttgatcg
     3001 gcgagatgtt ctctttggaa cagcgcacga agatgcaggg agtattctcc ggggtgtggg
     3061 gcgtctcatc gattgccggg ccgctcctgg gcggcttcat cgtggatcaa ctttcctggc
     3121 actggatctt ttatatcaat atcgttcccg gtctgctggc cgcggcgctg gtggcctttg
     3181 cctggcgcga tcaggtccac actcatgaac gacctgcggt ggattacgcg ggggcggcgt
     3241 tgctgactct tagtacggta ttccttctgc tcgggttgat ggaactcggc acatcccgcg
     3301 gctgggtctt cattacaggt tccattgtgt tctttgtcgt attactattt cttgaacgcc
     3361 gagtggctga tccgattctc ccgattccgc ttttccgcga cagcctcttt tccactgccg
     3421 ttgcacatgg acttttgact ggttgggctg tattcggaac gatctccttt atccccttgt
     3481 ttgttcaatc ggtgttaggc gcgaatgcca cacaggccgg tattaccatc acacccatgc
     3541 tcttgggctg ggtaagcggc agtatcgttg gcacgcgcat cctgctaagg gttgggtatc
     3601 gcaaattaag cctgattggc acgggtgtgt tcgcgatcgg tgcattcctg ttgatggttg
     3661 caggtgtgag ctcgagccag cttctgatca tggcttttgt gaccatgatg ggcatcggca
     3721 tggggctttc cattccgggc tacgtgattg cggtgcaaac aacagtagag aggcgtcacc
     3781 ttggcgttgc tacttcaatg cttcaattca gccgttcgat gggcggaacc cttggcgtca
     3841 gcgtgatggg ggcagctctg agcgcccgac tcgcgtcgaa cctcagtgct tcgggtctgg
     3901 accctcaaat ggttgagcaa ttgctcgagc caacgcccgg cgctgaagtc gtgatcaatg
     3961 ccgcagcgcg ggccgcgatg gcagatgcca tccatctaat ctttgtgatc gcgttcgtgg
     4021 cagctgcgct ggggctggtc tcggcgtttt tcacgccgcg caaagaactc actgatacaa
     4081 gatcttcaac aagagagagc atcccctcac ctgttagcgc agactgatca tcgaccttat
     4141 gaacaatcaa caaatcaatg aagcgtttga aagactttca accccattga ttgcggatgc
     4201 ctgtttgcgc ctggagattc cgatccggct tgcaccaaca ggcattcgtc ctatatcaat
     4261 tggatatcac attgccggcc gcgtcttgcc ggtgagacat tatgggagtg tggatatctt
     4321 tctggaagtc atggggaatt cacagcctgg cgatattctt gtcattgata acgatggcag
     4381 agtcgatgaa ggctgcatcg gcgatttaac cgcgctggaa gctcaggcca cagggctggc
     4441 tggcatcatc gtttggggtg tgcaccgcga tacgagcgaa ctcatcgaaa ttgactttcc
     4501 aattttcagc
//
LOCUS       DASTJP010000257         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1089972, whole genome shotgun sequence.
ACCESSION   DASTJP010000257 DASTJP010000000
VERSION     DASTJP010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089972"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..797)
                     /locus_tag="VFQ23_07820"
     CDS             complement(87..797)
                     /locus_tag="VFQ23_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906532.1"
                     /translation="MPVSLTSLAVPRTELSESEIQSMFRVFSENFNGSTLDIFERDLH
                     NKNWVILLRDGETKEIEGFSTLSLYETTFNHQRLSVVYSGDTIIRREYWGTPQLPSTW
                     IKTVLEKSTNMIQPLYWLLISSGYKTYRFLTVFYKEFYPRYDAPTPPDVQALMEHLAG
                     QRYGADYHCEDGVVRFQQGATPLREGIAEVTEERLHDPHVSFYIERNPGYVNGDELVC
                     ITRVHPDNFTPAGRRMAR"
     gene            complement(802..1767)
                     /locus_tag="VFQ23_07825"
     CDS             complement(802..1767)
                     /locus_tag="VFQ23_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET9906533.1"
                     /translation="MTTNTLSFPKSWYPLCRSSELKPRGVLKREAFGDAWVIFRTEQG
                     KVGAMHAQCAHMGADLSRGRVAGERLQCPLHEWEYNASGVCEHIPAEAVVPARARQIS
                     LKCKEQYGMVFGFLGGNPTFDFPQFENSDHDLYSAPYVMDFDTPYQVLAANSFDSQHF
                     ITVHHRRLLEPPSISSFSPNHFGVEFRARVEGGHFHDHLLRRIGVDVVELSAHCWGGN
                     NILAYNARTHARILFTILPVTDKKTRVYILNVMPKERAARLPRPIRRLILTIMHRLTI
                     AFLKSDIVVMRDLQFKLGVLLRDADHVFIQWVKYWKSLPIATGKG"
     gene            complement(1814..>2939)
                     /locus_tag="VFQ23_07830"
     CDS             complement(1814..>2939)
                     /locus_tag="VFQ23_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9906534.1"
                     /translation="LLDLLIGVGNRLIFLLFPFFRSRTGWENPRVRNITSLTYLLRGT
                     WIVNKNLDRWRKSRAQLFPVVLQVQTINRCNGKCGMCPYPYTVHLQPREVMDDALFTK
                     IVEECTKEEDFYEFVPMVQNEPLLDIRLEERIAEFKRHAQPHQAAEIVTNGTGLTPGR
                     FEKLVKSGLDTITISLSAFSQATYEKLIEGLSWAQVTKNLEAVAASPALAKVNIFLRY
                     VRQHGNESEFRPFKKYWEGRGLNVANYEINNRSGTLQEYETRQPVKSFLFKQARKSMG
                     RKVFKGACPHAFGIMHVLANGDVPLCANDWHNRHILGNVRQQSIREIYNSPQMDRYRE
                     LMEQGRFDEIDACRDCSFWQEWLKPDTRPLPRAEETSFSH"
BASE COUNT          736 a          788 c          706 g          709 t
ORIGIN      
        1 ttgttcgaca tctctggctg cgcgctgaaa acgtctccac cccggcaaac acgaggtaac
       61 ccacagcgcg ttggcaagcc tcgtcttcat cgcgccatcc gcctgcctgc gggcgtgaag
      121 ttatcgggat gcacgcgcgt gatacaaacg agttcatcac cattgacgta acccgggttc
      181 ctctcaatgt aaaaggagac atgcggatca tgcaagcgct cttcggtcac ttcggcaatg
      241 ccttcacgca atggggttgc gccctgttga aaacggacca caccgtcctc gcagtgataa
      301 tccgcgccat atctttgtcc tgccaaatgt tccatcaatg cctgcacatc aggaggagtt
      361 ggcgcatcat agcggggata aaactccttg tagaaaaccg tcagaaagcg ataggtttta
      421 tatcctgatg aaatcaataa ccaatacaat ggctggatca tgttcgtaga tttttcaagc
      481 acagttttga tccacgtgct ggggagctgc ggagttcccc aatactctcg ccggatgatc
      541 gtatcgccgg agtaaacgac gctcagccgt tggtgattga aggttgtttc gtaaagtgaa
      601 agcgtggaaa agccttcgat ctctttcgtc tctccatcgc gtaataagat cacccagttt
      661 ttattatgga gatctcgttc gaagatatcc aacgtcgaac cattgaagtt ctcactgaac
      721 acccgaaaca tggattgaat ctctgactct gatagctccg tgcgcgggac ggcaagactg
      781 gtcaacgaca caggcatcag actatccctt gcccgtagct atgggcaaag acttccaata
      841 ctttacccat tgaataaaaa catggtcagc atcgcgcaat agcacgccca gtttgaattg
      901 caaatcgcgc atcacaacaa tatccgattt cagaaaggca attgtgagtc tgtgcatgat
      961 ggtcaggatt aaacgccgga tcgggcgcgg caaccgggct gccctctcct tcggcatcac
     1021 gttcaggatg tagacacgag tctttttatc ggtgacaggc aaaatcgtaa acaggatgcg
     1081 cgcatgggtc cgcgcgttat aagccaggat attattccca ccccagcagt gcgcgctcaa
     1141 ttcgactaca tccacgccga tacggcgcaa gagatgatca tggaagtgtc ctccttcgac
     1201 acgcgctcga aactcaacgc caaaatgatt cggcgagaaa cttgaaatcg aaggcggctc
     1261 aagcaatcgg cgatgatgca cagtgatgaa atgctggcta tcgaatgaat ttgcagccag
     1321 cacctgatac ggcgtatcaa agtccatgac atacggtgcg ctgtaaagat catgatcgct
     1381 attctcaaac tgcgggaaat caaacgtggg attgcccccc agaaaaccga agaccatgcc
     1441 gtactgttct ttgcatttca gcgagatctg ccgcgctcgc gccgggacga ctgcttccgc
     1501 cgggatatgt tcacacacac ctgaggcgtt gtactcccac tcatgtaacg gacattgcag
     1561 ccgctcgccg gcaacgcgtc cacgcgatag atcggctccc atgtgagcac actgcgcgtg
     1621 catcgctcca actttcccct gttcggtgcg aaaaataacc catgcatcac cgaaggcttc
     1681 gcgcttgaga acccctcgcg gctttaactc cgacgatcga cagagcggat accatgattt
     1741 tggaaatgaa agtgtgttag tggtcaaggg tcatcctctg aatttgtgat ttcactcatc
     1801 atcaacactt ctctcaatga gaaaaggacg tttcctctgc tcttgggaga gggcgagtat
     1861 ccggcttcaa ccattcctgc cagaaggaac aatctcgaca ggcatcgatc tcatcgaagc
     1921 gtccctgttc catcaactcg cgatacctat ccatttgggg cgagttgtag atttctcgaa
     1981 tcgactgttg gcggacgttg ccaagaatgt gacgattatg ccagtcgttg gcacacagag
     2041 gcacatcgcc attggcgaga acgtgcataa tgccaaaggc atgcgggcac gcgcctttga
     2101 acactttgcg gcccatactc ttgcgtgcct gcttgaaaag aaagcttttc accggctggc
     2161 gagtttcata ttcctgcaat gtccctgagc ggttgttgat ctcatagttt gcgacattaa
     2221 gtccacgccc ttcccaatat ttcttgaaag ggcgaaactc actctcattc ccatgctgtc
     2281 gcacataacg cagaaaaata ttcaccttcg ccaacgctgg agatgcagca acagcttcca
     2341 gattttttgt cacctgcgcc caggataatc cttcgatcag tttctcatac gtcgcttggg
     2401 aaaatgcact caggctgatg gtaattgtat ccaaaccgct tttcaccaat ttttcgaatc
     2461 gaccgggcgt caatccggtc ccgttggtga cgatctctgc agcttgatga ggctgggcat
     2521 gccgcttgaa ctctgcaatg cgttcttcca gcctgatatc taataagggc tcattttgca
     2581 ccatcggtac gaattcatag aagtcttctt cctttgtaca ttcctctacg attttggtga
     2641 aaagcgcgtc gtccatcact tcgcgcggct ggaggtggac tgtgtaagga taagggcaca
     2701 tcccacattt gccattgcag cgattaatgg tttgcacctg caaaacaaca gggaataatt
     2761 gcgctcgact cttccgccag cggtcaagat tcttattgac aatccatgtg ccgcgcaaca
     2821 aatacgtgag gctggtgatg ttgcgcacgc gtgggttttc ccaaccggtc cgcgagcgaa
     2881 agaatggaaa gagcaggaag atcagtcgat ttcccacacc aatgagcaag tccaataaa
//
LOCUS       DASTJP010000258         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_85200, whole genome shotgun sequence.
ACCESSION   DASTJP010000258 DASTJP010000000
VERSION     DASTJP010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85200"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFQ23_07835"
     CDS             <1..892
                     /locus_tag="VFQ23_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906535.1"
                     /translation="GAALFAGGLIFHLRGSLRWVFLAGLLVSGVVTQYFSAADYRDFW
                     RVQREAWWQLAWRAPQIQDGTTLVVASLGGYQLPEEYEVWGPANMIYRPGSKLTIPGQ
                     VLFSDIWIDLANEEKENRAVRGTVRVPRDYGKVVILSQPTHGECLHLLDPAHPEQWIA
                     ESAQVQSIAQYSDIDLINTTEAGVVPPVKVFGAEPEHDWCFYFQKIELARQRGDWQTA
                     AALADEALDRGLLPSQIAEWLPLLDAYVHVGDLDKTDLIVKEMREDKRAHLGLCTHLK
                     ALNAQSGEVDPRFDLICSHD"
     gene            957..1901
                     /locus_tag="VFQ23_07840"
     CDS             957..1901
                     /locus_tag="VFQ23_07840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9906536.1"
                     /translation="MIPEQRTRIDLVIPVYEEAGVIEQIFKQIRVVVDALPYDFHVFL
                     VNDGSRDKTAESLRTIADEDPRVTVLSFSRNFGHQAALTAGLDHSTGDIVITLDGDGQ
                     HPPEMIPQMIDLCKQGYDIVQAQRIEDGQGFSFKQATSSAFYRLINAVSGTHIEPGAA
                     DFRALNRQAVDALKSMPEYHRFLRGMISWMGFRSVILPYHETHRVAGRSKYSFGKMFR
                     LAMDALFSFSLVPLYIGMTLGGILLCLAAAQIIYVLSFWFTGRSSELVAGWSSLMSVT
                     LVIGGMVMILLGFIGVYVGYIFQEVKKRPVYLLKKDEK"
     gene            1898..2566
                     /locus_tag="VFQ23_07845"
     CDS             1898..2566
                     /locus_tag="VFQ23_07845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9906537.1"
                     /translation="MSSAEKPIYHFVFLRHGESVGNVESRWQGQSDYPLTERGQAQAQ
                     ALARRWQEEKAKFDLVITSPLIRAKNTAEIVASALDVKVESDPLWMERNLGEITGLTI
                     DEIRTRPRPSFVTPYDTIGGVGEGDWVLFLRAGQALQSLLRRPAGRYLVVSHGGLLNQ
                     LMHAIVGVAPHADPSGVRFRFDNTAFARVFYLSHQHRWAIETLNDHAHLKSVIHNGDL
                     QQGP"
     gene            2582..3664
                     /locus_tag="VFQ23_07850"
     CDS             2582..3664
                     /locus_tag="VFQ23_07850"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HET9906538.1"
                     /translation="MINDINGKLNVLTFGAGAIGTYIGGSLALAGHQVVFLERPAKVD
                     ELRARGLRLNLTLDKRRNTKDAFHILSPGFVVASSLEDAFRYGPFDVALFALKSFDIQ
                     PALESLQPFAEQMPPVLCLSNGISNENAIAEVLGKDKVIYGTVTTAVGRRGSGDIVLE
                     KLRGVGVAKGHALSEKLVAAFDKAFLKPQLFEDAASMKWSKMLTNLIANPTSAILDMT
                     PAQIFANKDLYKLEIDMLKECLAVMEAQGIEVVDLPGTPVRALAFATKLPLWISRPLL
                     SRAAGAGRGGKMPSFHIDLHSGRGKSEVQYLHGEVVCAGEKFGVPTPVNKVLTETLLA
                     LVNKQIPLDEFAHKPEKLLEKASVVE"
BASE COUNT          928 a          928 c         1122 g         1011 t
ORIGIN      
        1 gggcgcggct ttgttcgcgg gtggcctgat attccatctt cgaggcagtc ttcgctgggt
       61 atttttggcg ggcttgctgg tctctggtgt agtgactcaa tatttcagcg cggcggatta
      121 ccgcgatttc tggagggtcc agcgcgaggc gtggtggcag ttagcgtggc gggcaccaca
      181 aattcaggat ggaacgacat tagtggtggc atcactgggt ggttatcagt tgccggagga
      241 gtatgaagtt tggggtcccg cgaatatgat ctaccgacct gggagcaagc ttacaattcc
      301 cgggcaggtg ctttttagtg atatttggat tgacctggcg aacgaagaaa aagagaaccg
      361 tgcagtgcgc ggtacggtgc gcgtgccgcg tgattacggc aaagttgtaa ttctctcgca
      421 gcccactcac ggcgagtgcc tgcatttgct tgacccggct caccctgagc agtggattgc
      481 agagtccgct caggtgcagt ccatcgcgca gtattcggat atcgacctga tcaatacgac
      541 cgaggcagga gtcgttccgc cagtaaaggt cttcggcgcc gagccggaac atgactggtg
      601 tttttatttc cagaagatcg aattggcgcg tcaacgtggc gactggcaga ctgcggcggc
      661 gctggcggat gaagccctgg accgtggatt gctgccctca caaattgcgg aatggctgcc
      721 gctcctggat gcttatgtac acgtgggcga tttggacaaa accgacttga tcgtcaaaga
      781 aatgcgcgag gataaacgcg ctcatttagg tctttgcacc catctaaagg cgctcaatgc
      841 tcaatcgggg gaggtggatc cgcgttttga tctcatctgc tcccacgatt agagtgatgc
      901 aaacgctggt tttgaaggaa taatgccgtg aaaagcagcg tggtagaata attctgatga
      961 ttccggaaca aaggaccaga attgatctgg taattcctgt ttatgaagag gcgggtgtaa
     1021 ttgagcaaat attcaagcaa atccgcgttg tggtggatgc cctcccttat gattttcatg
     1081 tatttttagt caacgacgga tcacgcgaca aaacggccga atcgcttcga accattgccg
     1141 acgaagaccc gcgcgtgacc gtcctctcgt tcagccgcaa cttcgggcat caggccgcgc
     1201 tgacagccgg gctcgatcat tccaccggcg atattgtcat cacgctggac ggtgatggac
     1261 agcacccccc agaaatgatt ccgcaaatga tcgacctctg taaacagggg tatgacatcg
     1321 ttcaggcgca gcgcatcgag gacggtcaag gattttcgtt caaacaagcg acctcttccg
     1381 ccttttatcg attgatcaac gcagtcagtg ggactcacat cgagcccggc gctgcagatt
     1441 ttcgcgctct gaaccgtcag gcggttgacg ccttaaagtc catgcccgaa tatcaccgct
     1501 ttctacgcgg catgatctcc tggatgggtt ttcggagcgt gatcttgcct tatcacgaga
     1561 cgcaccgtgt ggctggcaga tcgaagtatt cgtttggcaa aatgttccgt ctcgcgatgg
     1621 acgccctctt ctcgttctcg ctggttccgc tttacatcgg catgaccctg gggggtattt
     1681 tgctttgcct tgccgcggca cagatcattt atgtgttatc gttctggttt acaggacgtt
     1741 cctccgaatt ggtcgccggg tggagctctc tcatgtcggt aaccctggtg atcgggggaa
     1801 tggtgatgat cttgcttggt ttcattgggg tttatgttgg gtatattttt caggaagtta
     1861 agaagcgtcc tgtatatttg ttgaaaaagg atgaaaaatg agttccgctg aaaagccaat
     1921 ttatcacttt gtttttttgc gtcacggcga gtcagtgggg aatgtcgagt cgcgctggca
     1981 ggggcaatct gattatcctc tcaccgaaag ggggcaagca caggcgcagg cacttgcacg
     2041 gcgttggcag gaggaaaaag cgaagtttga cctggtaatt accagtccgc tgattcgcgc
     2101 caaaaatact gcagagatcg ttgcttctgc actggacgtc aaagtggagt cagatccgct
     2161 ctggatggaa cgcaatcttg gcgaaatcac tggcctgaca atcgatgaaa tcagaacacg
     2221 ccctcgaccc agttttgtta ctccctatga caccattggc ggtgtgggtg aaggtgattg
     2281 ggttttgttt ctgcgagccg ggcaggcatt acaaagtctc ttgcggcgac cggcgggtcg
     2341 ttatctggtt gtgtcacacg gaggtctgct taaccaactc atgcacgcca tcgtgggcgt
     2401 tgctcctcat gcagatccct ccggggtacg attccggttt gataacaccg cctttgcgcg
     2461 cgtgttttat ttatctcacc agcatcgctg ggcaattgaa acgctaaatg atcatgccca
     2521 tttgaaatct gtaattcaca atggggatct acaacaaggt ccataagaca tctacgaaac
     2581 tatgattaac gacatcaatg gcaaattgaa cgtgttgacg tttggcgcgg gtgcgattgg
     2641 aacttacatt ggcggtagcc ttgccctggc agggcatcag gtggtatttc tggaacggcc
     2701 agctaaagtg gatgagttgc gggcgcgagg cctgagactt aacttaaccc tggataaacg
     2761 ccgcaataca aaagatgcat ttcatatcct ctcccctggg ttcgtggttg cttcttcttt
     2821 ggaagacgcg tttcgttatg gaccttttga tgtggccttg tttgccctca aatcgtttga
     2881 tatccagcca gcattggaaa gccttcaacc atttgcagag cagatgccgc cggtactgtg
     2941 cttgtcaaat ggcatatcca atgaaaacgc gattgctgag gtgctgggga aagataaagt
     3001 tatctacgga acggtaacga ctgccgttgg gcgacgcggc tcgggggaca ttgtgttaga
     3061 gaaattgcgc ggagtcggcg ttgcaaaagg acacgccttg tctgaaaaac ttgtcgcggc
     3121 ctttgataag gcatttctca aaccacaatt attcgaagat gcagccagta tgaaatggtc
     3181 gaagatgctg acgaatctga ttgcgaatcc gacttctgcg atactcgata tgactccggc
     3241 gcagattttt gccaataagg atttgtataa gcttgaaatt gatatgctaa aggagtgtct
     3301 ggcggtgatg gaagcacagg gtatcgaagt ggtagacctt cctggtaccc cggtccgcgc
     3361 tttagcgttt gcgacgaaat tgcccttatg gatatcgaga cctcttttga gccgcgctgc
     3421 cggtgccgga cgtggcggta aaatgccatc tttccatatt gacttgcatt ccggtcgagg
     3481 taaaagcgaa gtgcagtatc tgcacggtga ggttgtttgc gccggagaaa agtttggcgt
     3541 gccaacccct gtgaataaag tattgacaga aacattatta gctttagtga acaaacagat
     3601 tccgttggat gagtttgccc ataagcctga gaaattattg gagaaagcat cggtggtcga
     3661 gtagagacga acagcgtgag gctcatatcg agactaccgg tagcaaccaa gagtaaattt
     3721 gagagcggtg gttgagtaga cctgcatgcc cgttgcaggg catatcgaaa ccacacgtga
     3781 ccatgaaaat taaacttgaa agtggtggtc tcgatatggt cgataaaaaa ctcgacctac
     3841 tcgaccaccg aagcataaaa taatgaaagc attcaacatc atttcccctc gtgacgaagt
     3901 cgaaatccac ttgccagatg ggcgcgtgat ggctggtccg cgtggcgcga aggtggggga
     3961 tttcttatca gcactggatc atgacgcgc
//
LOCUS       DASTJP010000259         6809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_17284, whole genome shotgun sequence.
ACCESSION   DASTJP010000259 DASTJP010000000
VERSION     DASTJP010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17284"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2292)
                     /locus_tag="VFQ23_07855"
     CDS             complement(<1..2292)
                     /locus_tag="VFQ23_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HET9906539.1"
                     /translation="MFDTQFFQRSRAPRLALLRMFSFLMISALLISPIGMRASSAESA
                     MAAPPWCGTPIPDGTGSLPDGSDPSHPVGSFPHIPWYAMGCTLADIQARSEGRMEVRV
                     LGQSALGRDMYLVTINALDTPQQRRDFQAWENIRKIALTEPQRAQEMLNRAGDGVKVP
                     IFVQSGIHGNESEGVDAMFELVERLATTPYGADPEVDAILNHVVLLWNVVHNPDGRIA
                     NARPNGNNFDLNRDFLTQSQPETKAAVSVMQEWLPPDMLDLHGYVTPTLIESTTKPHN
                     PSIDYDLWLKWNASRIDANEVAMNAIGLAVTRPILDWCANGNPPPASGLCANGLPPGP
                     SQAEGWDDWGPFYTPMYSQLVGLNGSTVEMCNSTGTGCGVPGSTTHPRGRLGAKIAQY
                     TVVWSTLLFDLENRHDLLWDELETYKRGVTNAPRPACCEPPYESDGFWMREYPKAYVI
                     PLGAGQRSDPEANRLVDWLLVNGIQVDEMKQDYSYAGTTFAKGSYVVWMTQAHRGLAD
                     TALSIGTNISGIIGQLYAPPGAWSHGYLWGADTLVIPRDATFAPITNRVTKSSHLLGG
                     VEPGVADAYVLAIDSPTAVRALNTLLSNGMPAQLAMTSFVSSNGEVLPAGSAIFAADS
                     ATKVALANAGRTNDVWFVRAYSDGLPAVEAIDRVPRIAVLTGVVNQDVWSLRNLGFVA
                     DPISTATINTAATDPLVNYDVIFNTGAYPAASNVTGRARLQAFFAAGGGYIGAGTNGA
                     TFLTTGTLVTGLTAASRTGNGRSG"
     gene            2740..3162
                     /locus_tag="VFQ23_07860"
     CDS             2740..3162
                     /locus_tag="VFQ23_07860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9906540.1"
                     /translation="MKTAIHDQVQHINPDGLNKNPAFTNVIAVTGPAKTIYIGGQDSI
                     NASGEIVAKGDIKGQTEQVLKNIQTALDAAGAGLEHIVKWNILVVQGQPIQPGLEAFQ
                     KVWGNRPNPPTITMAFVASLANPDFLVEMDAIAVVPER"
     gene            3507..3944
                     /locus_tag="VFQ23_07865"
     CDS             3507..3944
                     /locus_tag="VFQ23_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906541.1"
                     /translation="MNSSKFSKFAGIVMMVAGFLLFTATWGSHQFWAFISSLGLMGDL
                     THPLLSWLGLVVMAIGLFVLYRQLSPNQRLTSRLAFGVTAVGIGVAMFFTLGLVVPMP
                     ILESIGPVFAISSFLLMFAGSWIVIVLSVRRSDTEDQIKGLIP"
     gene            3999..4526
                     /locus_tag="VFQ23_07870"
     CDS             3999..4526
                     /locus_tag="VFQ23_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906542.1"
                     /translation="MKDTTISTFAYAAAGFFIATMVIAIGIGLPEFEGTPPLSVNLFT
                     LFGLIFHLAMLPVIAALPAPLWAKVSGFTWVVVDNMLILLSFYSAREEIVTPLRMGVH
                     LATATWIFGASMSHTGALRWVGFIVVIAMAGVSLIGPFLGNAVVQASGPGALLLIIWI
                     FMVGNHLGRSNAVAQ"
     gene            4545..5429
                     /locus_tag="VFQ23_07875"
     CDS             4545..5429
                     /locus_tag="VFQ23_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9906543.1"
                     /translation="MLKVGEFSRLSQVTIKTLHHYDELGLIKPAHIDPITNYRFYTLE
                     QLPRIHRIMALKEMGLSLEQIGLMLDQALSTEQMRGMLHLKQAEIQQEMREAQRQLAM
                     VEFRLRMIEAEINFPELDVVIKKLEAMRFLSFFAVSHATMEKALTYRAIVVSTLQKAI
                     ADGVIQHTGVVIDVFHGETIIPFTSPEIKDRQHEILLGVKNSQEAVKLKGIGQLTVKE
                     EPTVETAATLMLESSDLMDINLAGYVEKATLLRRWAIAHGFRPQDLVRYLSHRGILDS
                     TNPAEYVIEAQLPVDQGD"
     gene            5549..6202
                     /locus_tag="VFQ23_07880"
     CDS             5549..6202
                     /locus_tag="VFQ23_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906544.1"
                     /translation="MNTTSDSTNPGHPFVAGFWLMIAIPIFAFIVLPILMRLGVDDLY
                     TLPGLIVMFAVHFASGFMWARSLGMRSGMPGNRTMNIFGGLGFALGVVGTLILYTSGS
                     TVADAINRWVESFEGVGNLEFGALFAPWTGLACGVSGFALGIGLKGWKLALKLLVLGF
                     ITGLGLYLAIMFTMELLGFKVGSGRPVMLPTTFLSMWSAALVGSALFGKMLTKSKTS"
     gene            complement(6195..6395)
                     /locus_tag="VFQ23_07885"
     CDS             complement(6195..6395)
                     /locus_tag="VFQ23_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906545.1"
                     /translation="MNKLNRPVQGVGTSPRLGRLIGVSVYPFATLAPGASAGEHPCGA
                     PNREGMELAFSVFATVLAPKTN"
     gene            6604..>6809
                     /locus_tag="VFQ23_07890"
     CDS             6604..>6809
                     /locus_tag="VFQ23_07890"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906546.1"
                     /translation="MKQHAQMMVEHIQANKPGTVAFLHYLNEEETELSIVHIFPDAEA
                     YDLHLQGVGERAKGAFEFIEAIRR"
BASE COUNT         1646 a         1712 c         1797 g         1654 t
ORIGIN      
        1 accactgcgt ccatttcccg tgcgactcgc ggcggtgagt cctgtcacca gcgtaccggt
       61 ggtcaggaag gtagcgccgt ttgtgccagc accgatatag ccaccacccg cagcaaaaaa
      121 ggcttgcaac cgggcgcggc cagtaacatt tgaggctgcc ggataggccc ccgtgttgaa
      181 gatcacatcg tagttaacca gggggtcagt ggcagcggtg ttaatagttg ccgtggagat
      241 ggggtccgct acaaatccta aattgcggag cgaccagacg tcctggttga ctacccccgt
      301 taggacagca atccttggaa cacgatcgat tgcttctacc gcagggagtc cgtcgctgta
      361 ggctctcacg aaccacacgt cattggtgcg accagcattc gccaacgcaa cttttgttgc
      421 ggagtcagcc gcaaagatcg cgctgccggc aggcaaaacc tctccgttgg agctgacgaa
      481 ggaggtcatc gccagttgcg ccggcatacc gttgctgagc agggtgttga gtgctctaac
      541 ggcggtgggc gagtcgatgg caagaacgta agcatcggcg acgccaggtt caaccccacc
      601 cagcaggtga cttgacttcg tcacacgatt ggtgatgggt gcgaaggttg cgtcacgcgg
      661 gatgaccagc gtatctgcgc cccataagta gccgtgactc catgctccgg gtggggcata
      721 caattgccca atgattcctg agatgttggt accgatgctc agtgcggtat ccgcgagtcc
      781 gcgatgcgcc tgggtcatcc ataccacgta agagcctttg gcgaacgtcg taccggcgta
      841 tgaataatcc tgcttcattt cgtcaacctg gatgccattg acgagcagcc agtcaacgag
      901 ccggttggct tcagggtcgc tgcgctggcc ggcgccaagt ggaatcacat aggctttggg
      961 atactcacgc atccaaaacc cgtcgctttc ataggggggt tcacagcagg cgggccgcgg
     1021 tgcatttgtc acaccacgtt tataggtttc aagctcgtcc cacagcaaat catggcgatt
     1081 ctccaggtcg aagagcagcg ttgaccagac gaccgtatac tgagcaatct tagctcccag
     1141 ccgaccgcgc ggatgggtag tacttcctgg cactccacat cctgtacctg tagagttaca
     1201 catttcaacc gtggagccgt tcaaccccac caactgcgaa tacatcggtg tatagaaggg
     1261 gccccagtca tcccaacctt ccgcctgaga gggacctggc ggcaggccgt tcgcacacaa
     1321 tccactggca ggtggagggt tgccattcgc gcaccaatcc agaattggtc gcgtcaccgc
     1381 caaaccaatg gcgttcatgg cgacctcatt cgcatcaatg cggctcgcat tccatttcag
     1441 ccagagatca tagtcaatgc tcgggttatg cggcttggtt gtcgattcga tcagagtggg
     1501 cgtaacgtat ccgtgcaaat cgagcatgtc gggcggcagc cattcctgca taacggagac
     1561 ggcggctttt gtctcaggct gagactgtgt caggaagtcg cggttcaagt caaagttatt
     1621 gccattcggg cgggcgttcg caatgcgtcc gtccgggttg tgaacgacat tccaaagcag
     1681 aaccacatgg ttcagaatcg cgtccacttc cggatcagct ccatagggag ttgtagcgag
     1741 tcgctcaacg agctcgaaca tcgcgtccac cccttctgac tcgttgccat ggatacccga
     1801 ttgtacgaag atcggaactt tcacaccgtc gccagctcga ttaagcattt cctgagcccg
     1861 ctggggttcg gtaagcgcga tcttacgaat gttctcccag gcctggaagt ctctgcgctg
     1921 ttgaggggta tcgagggcat tgatcgtgac cagatacata tctcgaccga gggcggactg
     1981 cccaaggact ctcacttcca tgcgtccctc gctgcgtgcc tggatgtctg cgagcgtaca
     2041 acccatggca taccacggaa tatgggggaa gctgcccacg gggtggctgg ggtcgctgcc
     2101 gtctggcagg gatcctgtac catcggggat aggcgtgcca caccacggtg gcgcggccat
     2161 cgcgctctct gcggatgaag cgcgcatccc gatgggcgaa atcaacagag cggagatcat
     2221 caggaacgaa aacatacgaa gcaaagccaa tcgtggggct ctgcttcgtt gaaaaaactg
     2281 cgtatcaaac attgtcattc tcctctgaat gcatacatga atacaaatcc tgcgtgggta
     2341 attgcgtggt ctgttctcct cctaacatca acaatgctcc gcctatttca ccgcctttct
     2401 ttttgaattt atccggtaac gctcggccat tcgaacaaca tcgttttttt gctgagctcc
     2461 aaattacaaa acgattgtaa ctctctgaaa aaccagtgtc aataaattga gagaggggtt
     2521 taattttaga cacataactg gaccatggat gacccatcac aatgaaccac aagtcgaaat
     2581 taatttccac ttctgctcga ttaagggatt tcccccattg tcggtcactc taccgatgca
     2641 gtagattaat tactgtccat ttgtataaaa tccacagcgt tcaatcgaaa actactcaat
     2701 ggtaggtaat ttgtcatttt gtagaaaaag gagtaaacca tgaaaactgc catacatgac
     2761 caagttcaac atattaaccc ggatggcttg aataaaaacc cggcgttcac gaacgtaatt
     2821 gctgtaacgg gacccgcaaa aacaatctat atcggtgggc aggattctat aaacgcatcc
     2881 ggagagattg tggcaaaggg tgatatcaaa ggacaaactg agcaagtatt gaagaacata
     2941 caaacggcct tggatgcggc cggagccgga ctggagcata tcgtcaaatg gaatattctc
     3001 gtcgttcagg gacaacccat ccagccggga ctagaagcct tccagaaggt atggggtaac
     3061 cgaccaaacc caccgaccat tacgatggcg ttcgtagcca gcctggcgaa tccagatttt
     3121 ctggttgaaa tggatgcgat tgcggtggtt cctgaaaggt agaaaatttt tattcatcgt
     3181 atcggagtgg ccagtgtgat ttggttaatc cataacccct ccatgaccta aatcagggaa
     3241 taagggcgag gtccctgaaa accgaaaagg acggatagaa gcagagtatt ttctgaaggc
     3301 attgctgcat tgctacactg gtccataaac taaaaccagc actaggggaa atgaaaagac
     3361 acatctttca agctaacctg aaaatccacc aacaattccg ataaatcagg tcttgactct
     3421 cctgttaccg gaggatgtag actgacaata gtttacatac gctttggtgc agtgtgttgg
     3481 accaacacct gcgaaaggag actttcatga attcatcaaa gttttctaaa tttgccggta
     3541 tcgtaatgat ggtcgctggc tttctgctct tcaccgccac ttggggtagt caccagttct
     3601 gggcattcat ttcgtccctt gggctgatgg gcgaccttac gcacccacta ttgtcctggc
     3661 ttggacttgt ggtgatggca attggtcttt tcgtactgta ccggcaatta tcgccaaatc
     3721 aacgtttgac gagcaggctg gcttttggag tgacagccgt aggtattggc gtggctatgt
     3781 ttttcactct tgggctggtt gtcccaatgc ccatcctgga aagcatcggt ccggtttttg
     3841 caatatcatc cttcctgtta atgttcgcag gcagctggat tgttatcgtt ctgtccgtaa
     3901 gaaggtcgga tacagaagac cagatcaagg gacttattcc ttagtaagct atcgacaaca
     3961 atcttaatat agaaaaccaa tcgatgggag aattacacat gaaagacaca accatctcaa
     4021 cgtttgctta tgctgccgca ggcttcttca tcgctacaat ggtcatagcc attgggatag
     4081 gtttgccgga attcgagggc acacctccat tatcggttaa tttgttcact ttgttcggat
     4141 tgatctttca cctggctatg ttgcctgtga ttgcagctct gccggcccca ttgtgggcga
     4201 aggtttccgg cttcacctgg gttgttgtgg ataacatgct gattttgttg tccttttata
     4261 gcgcacgcga ggagatcgtc acgccgcttc gcatgggtgt gcatctcgcc acggcaacat
     4321 ggatattcgg cgcaagcatg tctcacacag gcgcgcttcg ctgggtggga ttcatcgttg
     4381 taattgctat ggctggcgtt tcattgattg gtccattctt gggcaatgca gtcgtgcagg
     4441 catcagggcc gggagcgcta ttgttaatca tctggatatt catggtaggc aatcacctcg
     4501 gcaggtcgaa tgcagtagca caatgacttt ataattactc aaccatgctg aaagttggtg
     4561 aattctcacg cctgagtcag gtgacgatca aaaccctgca tcattacgat gaactggggt
     4621 tgatcaagcc cgcccacatt gaccccatca cgaactaccg cttctacact ctcgagcaat
     4681 tgccgcgcat ccaccgcatc atggcgctca aggagatggg actctcgctt gagcagattg
     4741 gcctgatgct tgatcaagcg ttatccaccg aacagatgcg cggcatgctg cacttgaaac
     4801 aggcggaaat tcagcaggag atgcgcgagg cgcagagaca actcgcgatg gtcgagttcc
     4861 gcctgcggat gatcgaagca gagatcaact tccctgagtt ggatgtggtc atcaaaaagc
     4921 tggaggcgat gcgtttcctg tcctttttcg cagtatcaca cgccacaatg gaaaaagcat
     4981 tgacatacag ggcaatcgtt gtgtccaccc ttcaaaaagc catcgccgat ggagtcatcc
     5041 aacataccgg ggttgtgatt gacgtctttc atggtgagac gatcatccct tttacatcgc
     5101 cagaaatcaa ggatagacag catgaaatat tgctcggtgt gaagaattca caagaggcgg
     5161 tcaagctgaa agggatcggt caactcacgg tcaaggaaga accgacggtg gaaacagctg
     5221 ctaccttgat gttggaatcg tccgatctca tggatatcaa tcttgcgggc tatgtcgaaa
     5281 aagcgacttt gcttcgccgc tgggcgatcg cgcacggatt tagaccgcag gacctggtcc
     5341 gctatttgag tcatcgcggc atcctggatt cgaccaaccc ggcagagtat gtgatcgaag
     5401 cgcagttacc agtggatcaa ggggattgat ccccggcaac gccaaaacaa taacgataac
     5461 ttgccttgtt caacaaattg gttcgaggtg caaacgcgcc ctggttctgt tcagcgcgcc
     5521 tgcgtgtgat acctaaaagg agtcgaccat gaatacaacc tctgactcaa caaatcccgg
     5581 gcatcccttc gttgccgggt tctggttgat gatcgccata ccaatatttg cgttcatcgt
     5641 gcttccaatc ctgatgaggt taggtgtaga tgatttgtat acactcccgg gattaatcgt
     5701 catgttcgca gtccattttg cctcgggatt tatgtgggcg cgctcactgg ggatgcgctc
     5761 aggtatgcct ggtaacagga caatgaatat ctttggcggg ctcggctttg cactgggagt
     5821 tgtggggact ctcatccttt acacatccgg ttccactgtg gcggatgcca taaatcgctg
     5881 ggttgaatca ttcgaggggg ttggaaatct tgagttcggt gccctcttcg ctccatggac
     5941 tgggcttgca tgtggtgttt caggttttgc attggggatt gggttgaaag gctggaagct
     6001 tgcgttgaaa cttctggtgc tgggattcat caccggattg ggtttatatc tggcaatcat
     6061 gttcacgatg gaactgctcg gcttcaaggt tggcagcgga cggcccgtaa tgttgccaac
     6121 aacgttcttg agcatgtggt ccgcggcgct ggtgggcagt gcactatttg ggaagatgtt
     6181 gacgaaatca aaaactagtt agttttcggg gctaacacgg tagcaaacac agaaaaagcc
     6241 aactccatgc cctctctatt gggagcccca caggggtgct cacctgcact tgcgccaggt
     6301 gcaagtgtcg caaaggggta aacggacacg ccgatcaatc gtcccaatcg gggtgaggtc
     6361 ccaaccccct ggactgggcg atttaattta ttcataacgc ttccatttct tgccgctgaa
     6421 tcaacgttaa tgtacaatgc tgcatattgc ccattgcaca ggattgaaat tccggataga
     6481 ggaattgcga aaaatgaacg ggtctgttaa aggtatgctc tttgccttag aaaggagaac
     6541 aagatatccg gacccatcgt tttcatcagc catttcaaga tcaaggaggg caacctcgag
     6601 ggcttgaaac aacatgccca aatgatggtc gaacacattc aggcgaataa acccggcaca
     6661 gttgctttcc tccattatct caatgaggag gaaacagagt tgagcatcgt ccacattttt
     6721 ccggatgcgg aggcatatga cctgcatctg caaggggttg gtgaaagggc gaagggcgca
     6781 ttcgaattca tagaggccat tcgccgaga
//
LOCUS       DASTJP010000260         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1377853, whole genome shotgun sequence.
ACCESSION   DASTJP010000260 DASTJP010000000
VERSION     DASTJP010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377853"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFQ23_07895"
     CDS             complement(<1..230)
                     /locus_tag="VFQ23_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906547.1"
                     /translation="MNKYAILSWLVMILIPIVLIGLGLRILLSPFFLRLEYNMPYFPP
                     DEYGFTKEDRLKWAPYALDYLVNNEDISYLAD"
     gene            complement(227..1777)
                     /locus_tag="VFQ23_07900"
     CDS             complement(227..1777)
                     /locus_tag="VFQ23_07900"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9906548.1"
                     /translation="MKRYILFTVFISGMTTLASELAAARLIGNVFGTSNIVWASIIGL
                     ILIYLTFGYFLGGKWADANPTPVAMYRVLAWGAFTLGLIPFIAGPVLRYAASAFDSLQ
                     VGILGASFVAVLVLFIVPITLLGTISPFAIRLSVDDTARAGQTSGQIYAISTLGSFIG
                     TFLPTLVTIPAIGTKNTFLLFSLILLTVALIGLARFASQREWLKYSWMPIALIVIAIF
                     SAGQSLKSSVGQIYETESAYNYIQVQEKAGFTLLRLNEGQGVHSIYNPDTLQYNGPWD
                     QFLVSPYFYTGRKPSDVKRVAIVGLAAGTTARQLTAVYGNIPIDGYELDPEIVEVGQK
                     YFGMTMPNLNVVIGDGRLNLERSEGQYDIIGVDAYRPPYIPPHMTTREFFEICASKLT
                     DDGVLTLNSASVPGDRRLINGLATTMATVFPSIYTVDIPGSLNTMIFATKQPTTPEDF
                     AANLVALSQDTSVHPLLLSTMQVTFANLKSGYEPTQVFTDDHAPIEWIVNDMVIRFIL
                     EGGTEYLQ"
     gene            2024..>2599
                     /locus_tag="VFQ23_07905"
     CDS             2024..>2599
                     /locus_tag="VFQ23_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HET9906549.1"
                     /translation="MYIPKLYREEDREKILEFLRQNDFATLVTYDGEKPVASHLLMGI
                     VEKGERLFVDGHMSRANPHWKTFDTNPEALVIFQGAHTYISPTWYNHVNVPTWNYQSV
                     HLYGKPRIITDYDEAYTLLKRLVDRYESSSHYELDSLPKDFVEKEIKGIVAYQIEVTR
                     IEANYKLSQNRKDEDYANIITQLELRTDEFSH"
BASE COUNT          730 a          577 c          656 g          636 t
ORIGIN      
        1 aaatcagcga gataggaaat atcttcgttg ttcacaagat aatccaacgc gtaaggcgcc
       61 cacttcaaac ggtcttcttt tgtaaagccg tattcatctg gtgggaagta tggcatgttg
      121 tattcgagcc gcagaaagaa aggtgaaagc aagattcgta atcctagacc tatgaggacg
      181 atgggaatta ggatcatgac cagccaggaa agtatggcgt acttattcat tgcaaatact
      241 ccgtcccacc ctcaagaata aaccgtatca ccatgtcgtt gacgatccat tcgatcggcg
      301 cgtggtcgtc ggtgaagacc tgcgtcggtt cgtagccaga tttgagattg gcgaatgtca
      361 cttgcatcgt ggaaagtaat agagggtgaa cacttgtgtc ttgtgaaagg gcaacaagat
      421 ttgccgcaaa gtcttctggc gtggtcggtt gttttgttgc aaagatcatt gtgttgagtg
      481 aaccggggat atcgacagtg tagatggatg ggaagacagt cgccattgtg gtcgcgagtc
      541 cgttgatgag cctacgatct ccagggacgc tggcgctgtt cagcgtaagg actccatcgt
      601 cagtcagttt ggatgcgcaa atttcaaaga actcacgcgt ggtcatgtgt ggaggaatat
      661 aaggtgggcg ataggcgtct acaccgatga tgtcgtattg accttcgctg cgttccaaat
      721 taagtcgtcc atcaccaatg accacgttca ggtttggcat ggtcatcccg aaatacttct
      781 gtcctacttc gacaatctcc gggtcaagct cataaccatc aatcggaata ttcccgtaaa
      841 ctgcagtcaa ctgccgggcg gtagtgcctg cggctaagcc gacaattgca acgcgtttta
      901 catcggatgg tttacgaccc gtataaaaat atgggctaac gagaaattga tcccagggtc
      961 cattgtattg tagagtatca ggattgtaga tcgagtgaac gccttgacct tcattcaatc
     1021 tcagcaaggt gaatccagct ttttcctgga cttgaatgta gttgtaggct gattcagttt
     1081 cgtagatttg ccccacactg cttttgagcg attgtcccgc cgaaaaaatt gcgatgacta
     1141 ttaatgcaat cggcatccat gaatacttca accattcacg ctgactagca aaacgcgcca
     1201 gcccgatcaa ggcaacggtc agcaaaatca aactgaataa tagaaatgtg ttctttgttc
     1261 caatcgcagg gatggtgacc aaggtgggca ggaacgttcc aatgaaagag ccgagcgttg
     1321 atatggcata tatctgacct gaggtttgac ctgcgcgtgc cgtatcgtcc acactcaggc
     1381 gaatcgcaaa cggagaaatc gtcccgagca aagtgatggg aacgataaac aggactaaca
     1441 cggcgacaaa agacgcgcct aaaattccca cttgcaatga gtcaaacgcg ctggctgcgt
     1501 accgtaggac aggacccgct ataaatggga tgagtcccag ggtgaatgcg ccccacgcga
     1561 ggacccggta catcgcaacg ggcgttgggt tggcatccgc ccatttgccg ccgagaaaat
     1621 acccgaaggt tagataaatc agaataagcc cgatgatgct cgcccagacg atattacttg
     1681 tgccgaatac atttccaatc aaacgggcag ctgcgagttc tgatgcaaga gtggtcatgc
     1741 cagagatgaa gacagtaaat aagatgtagc gtttcatggt tggaggatta taccgtttga
     1801 ggtgtaatcc ggtggttgag cgcagacttg agagcggaga aaagggaaaa attcttagcc
     1861 atagagagca ctgagtcctt tgagtaacta actcaaaaat ctctgtgggc tcggtggctt
     1921 acacacttag ttcgccaccc cccacacatg cgcatgactc agtgcaggtg ggcaatcgaa
     1981 cccgccagcg gcgatgatat actttatttt acaaaggagt tttatgtata tccccaaact
     2041 ttatcgtgaa gaagatcgag aaaagattct tgaatttctt cgtcaaaacg attttgcgac
     2101 tcttgttaca tatgacggcg aaaaaccggt cgccagtcac ttgttgatgg gcattgttga
     2161 gaaaggggaa cggctttttg tggacgggca tatgtcgagg gcgaatccac actggaaaac
     2221 attcgatacg aatcctgaag cgttggtgat ctttcaaggt gcacacacct acatttcccc
     2281 gacctggtac aaccatgtca acgtgccgac ctggaattac cagtccgtgc atttgtacgg
     2341 caaaccgaga attatcacgg attatgatga agcttataca ttactaaagc gacttgttga
     2401 ccggtatgaa tcaagcagtc actatgagtt ggactccctt ccaaaagatt ttgtggagaa
     2461 agaaatcaaa ggcattgttg catatcaaat tgaagtgaca agaattgaag cgaactataa
     2521 actgagtcaa aaccgtaagg atgaagatta cgccaacatc atcactcagc ttgaactgcg
     2581 aacggatgag ttctcgcac
//
LOCUS       DASTJP010000261         7233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_955376, whole genome shotgun sequence.
ACCESSION   DASTJP010000261 DASTJP010000000
VERSION     DASTJP010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955376"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFQ23_07910"
     CDS             <1..591
                     /locus_tag="VFQ23_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906550.1"
                     /translation="LVSRSGSELIQLQHNLEGNCFLKAMAGEWIPLQSSMVDAKLFHQ
                     LGGFSPLISGPEDIDLFRRVCLRGEIAETPNLIAKIERGDEDSTTNYVKHPELSRWAR
                     EKILDDVGAFVRMKDGTNSSYWFGKLLRIYITSAVWNMQRKRIFTVLSRAIYSLIIIL
                     LSNFRVLTTDFWMALLLSYQSPTFERGFRISGRKAR"
     gene            1035..1682
                     /locus_tag="VFQ23_07915"
     CDS             1035..1682
                     /locus_tag="VFQ23_07915"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9906551.1"
                     /translation="MSQFDMFGSAQPQSSSSKPSSLDELFDQMKHMKDDPLANAGTNI
                     VISRGNPKAKLLIIGEAPGPEENIKGKPFVGRAGQLLDKILESADFDPEKDVFITNSV
                     FRLPPGEDGKAFRKPTTEEIDFYRPFVLEIIRLVDPVIMLLTGNVACQSILEKTGITN
                     LRGKWTEMNGRWVMPIFHPSYLLRNPVREPGSPKALMWDDIKNVRKKYDELIGSK"
     gene            2118..5492
                     /locus_tag="VFQ23_07920"
     CDS             2118..5492
                     /locus_tag="VFQ23_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9906552.1"
                     /translation="MKTKLTFFYRAASRWIGVGVLTIGLLVFIGWLLSIPVLTSMFSQ
                     ANMKANTALGFILCGITLWCINYDQVTERMRLIANACALITLLLAALTLGEYATSSDF
                     GIDQLFIRDSTAVSGASFPGRLSPVTALNLLLLSSALLLLNNNRAHWLSESLTIMVII
                     IAGVSLIGYIYGVSSLYRIGINSPIALITILTFILLGLGILCSQPEYGLIRLLSLEDR
                     SGILARRLLPAAIGIPILLGWLFLSGERAGYYQSSFGIALFALSSILVFSALIWRNSR
                     LLSTVDAARGQAEEALSLSEKRAQALIENSWDAIALFSADGRILYGSPSTPQVLGYSL
                     SEFVGRNAFELIHPEDQALATERMNLSLQQPRAHIPVNARVLHKSGEWRWLEGVFTNL
                     LDEPSIRAIVNNYHDFTERKRDDDALRESESYSFSLLSLSKKLEQAQTYSEALNAALE
                     QVKALLGYQNVWMYLLSDDKQSLRLLTSTGDLSQKISEDFATLPIKGDRFLAEIAEGK
                     EPVLVEDALTDSRTNKDIVGQLGNRTIVNVPIVLMDRHLGAFGTGSFGEEGVRVPTTV
                     QLEYLRSLASHMAVTIDRIHLLTERRQSEEQIAYQAYLLENVNDAVIGSDENTLIRFW
                     NQGAERIFGWKAEEVLGRSAREVLRSEMINTDRETVLKKLAEQGRWRGEAALYRKDGT
                     KVIMEVSSITLRDTHGLLTGYVSVNRDITDRKHIEAEIRKLNTELEQRVVERTAAFSQ
                     ANSLLQMMLDHMPDQIYFKDAQSRFIRNSRSQATALGLNDPADIVGKSDFDFFPHAQQ
                     SFEIEQEIMRSGTPIVNQEELVRWPDGTETWVSTTKVPLRDQAGDIVGTFGISRDISE
                     RKRAELALQKAKLELEAANKELEAFSYSVSHDLRAPLRSVDGFSQAILEDYGDILPAD
                     GREFLERIRSATQRMDRLIDDMLNLSRVTRAPLKSAPVNLTKLAEDIAAELRQYDTNR
                     NVNFVIAQNLRSNGDPRLLQIVLENLMRNAWKFTSKRELGEIEVGAREENGETVYFIR
                     DNGAGFDMTYANKLFGAFQRLHAMTEFPGTGVGLATIQRIIHRHGGRVWAVGVVDQGA
                     TFSFTLPILASPKPVTPVSEMKEEDESIHRRAREII"
     gene            5506..6279
                     /locus_tag="VFQ23_07925"
     CDS             5506..6279
                     /locus_tag="VFQ23_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906553.1"
                     /translation="MTTESTLIFSAFIALTAAVIYAYVGWRLGKRVITSSEGRLAWVA
                     FTLWWYGLALTTLIGGIQNLLGAIGITSLSLFVTATYVNIFPACIALWGLLYYLLYLF
                     TGNRRSLLPLTLFYVIYYILLVYYTTASIPEGVEVGRWSTTLIYRTPITGPFFIILVV
                     LLFFPQIIGSIAYFTVYFRVTDPTQRYRILLVSGSIMVWLLSPYVALAGGVTENDWWH
                     LASRFIGLAAPLTVLMAYLPPYWLKQRYGIISLSDEPKQ"
     gene            6317..6760
                     /locus_tag="VFQ23_07930"
     CDS             6317..6760
                     /locus_tag="VFQ23_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9906554.1"
                     /translation="MEEKMILLVEDNPDDEALTLRALKKHNIGNKVFVVRDGAEALDF
                     LFCTGKYSDRDPNDKPQVTLLDLKLPKVDGLEVLRRLRADERTQTLPIVILTSSNEEK
                     DLIQGYKNGANSYVRKPVDFNEFLEAVRDLGLYWLVWNYAPPRTK"
     gene            6767..>7233
                     /locus_tag="VFQ23_07935"
     CDS             6767..>7233
                     /locus_tag="VFQ23_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9906555.1"
                     /translation="MRKPLRVLILEDSEDDSLLLLRELKRSGYEVEFERVDTASAMRS
                     ALTRKTWDLILSDYSMPDFNAPKALELLKTSELDLPFIIVSGTIGEETAVAALKAGAN
                     DFLVKGKFSRLGPAIERELREAESRNDRRRAEEQLRYQANLLANVTDAIIAAD"
BASE COUNT         1830 a         1622 c         1827 g         1954 t
ORIGIN      
        1 ctggttagtc gcagtggcag cgaattaatt cagttgcaac acaatttaga aggtaactgt
       61 tttctgaagg caatggctgg cgaatggatt ccgcttcagt cttcaatggt tgatgcgaaa
      121 ttgtttcatc agctgggcgg attttcgccg ctgatcagtg gtccagaaga catcgacctg
      181 ttccggcgcg tttgtcttag gggagaaata gccgaaaccc ccaatttgat tgcaaaaatt
      241 gagcgaggcg atgaggacag cacaacgaat tatgttaaac atccggaatt gagtcgctgg
      301 gccagggaga aaatcctgga tgatgtgggt gcgtttgtcc gtatgaagga tgggacaaat
      361 tctagttatt ggtttggtaa gctgttgcgg atctatatta catctgcagt atggaatatg
      421 caacggaaac ggatttttac tgtactcagt agggcgattt atagtttaat tatcattttg
      481 cttagcaatt ttagagttct taccacggat ttttggatgg ctctactcct ctcctatcaa
      541 agcccaacat ttgaacgggg attcagaatt tcaggcagaa aagcgcgata agcgcgtcaa
      601 aggattatcg cgcatgaatg tgttcgtgtt cacgatcatc cagatgattg gtagggaaac
      661 gctttcgcgc gcggagcacc gcgggtcatc agtcaattgg ctggttggtt tcagaatgag
      721 atcagtaaat taccacagta tggcggtcta aacttaactt attcttccac tctagtgggc
      781 gaaccttaca ggtgacaacc ttacgagcga caatttcttg agaaaataac caaataccct
      841 ttcgctatgg atgctgattt ctaacaggat tgtacgtctg tcgcgtcttc cgcttacgtt
      901 tttaaaaaga ccacgatgaa tcgtggtctt tccttttaag gtttaatgag tttgttcgct
      961 aaacttttat cggcaatgaa tcaaaagcat aaggtacaat tctctctgca ctcaaaggat
     1021 tataggagtt cgatatgtct caatttgata tgtttggttc agcgcagcct caatcaagct
     1081 cgtctaaacc ttcgtcgctt gacgagctgt ttgatcagat gaagcatatg aaagatgacc
     1141 cgcttgccaa tgctggtacg aatattgtta tcagtcgtgg aaacccaaaa gcgaagttgt
     1201 tgatcatcgg tgaggcgcct ggtccggaag aaaacatcaa aggcaaaccg tttgtcgggc
     1261 gtgctggtca actcctcgat aagattcttg agtccgctga ttttgaccct gagaaggacg
     1321 tgttcatcac caactccgtc ttccgcctgc cgccagggga ggatggaaag gcatttcgta
     1381 aacccacaac agaggagata gatttttatc gtccattcgt attggaaatt attcggctcg
     1441 ttgaccctgt tattatgttg ttaactggca acgtcgcctg ccaatccatc ctcgaaaaga
     1501 ctggaattac caaccttcga gggaaatgga ctgaaatgaa cggacgatgg gtcatgccga
     1561 tcttccatcc ctcttatttg ttgcgaaacc ctgtgcgcga acccggctcc cctaaggcat
     1621 taatgtggga tgatatcaag aacgttcgta aaaagtatga tgaattgata ggaagtaagt
     1681 aacagatttt tgataggaat tttctaccct aattcagcta ttgctccttt atggcatcct
     1741 ttgcttacct ggctatctgc gcgataagtt gacttgagca tgtgggggat aaaaggccca
     1801 tcgcaaattc gtgagtgttc caatcgcgac tgccagggtc agcctctcga cccgttcgag
     1861 tggcttgaat cattgcagcc cactttgcag cctaccatca cataaaatca tattccaaga
     1921 gtgtaggctg ttagtgcact cgaaatgtct ttcaagttat cagacttatt gcctgcttga
     1981 tctctagcta ctgctgtgga tttagtgaga acatagggcg ataggtcgat tcatgatttt
     2041 attgaatacg ataaagatcg caggttatca gagtgtgaac atgactgatc tgtgtgacat
     2101 cgttttatga cgtcgtgatg aaaaccaaac tcactttttt ctatcgagct gcttcgcgct
     2161 ggatcggcgt gggcgttctg accattggct tgttagtctt tatagggtgg ttattaagca
     2221 ttccagtgct gaccagtatg tttagccagg caaacatgaa ggccaatacc gcactggggt
     2281 ttatcctttg cggtattaca ctttggtgca tcaattatga ccaggtgact gaaagaatgc
     2341 gactgattgc aaatgcctgt gcattaatca ctctcctcct cgctgcgctg acgctggggg
     2401 aatacgccac gagcagcgat tttggcatcg atcaattatt catccgcgat tctactgctg
     2461 tttcaggcgc ctcctttccg ggtcgtttgt ctcctgtcac tgcgctgaat ctgctattac
     2521 taagcagcgc actgttacta ctcaataaca atcgggctca ttggctcagc gagtcactaa
     2581 ccatcatggt aatcatcatc gctggtgtaa gtttgattgg ttatatctat ggggtttcct
     2641 cgctttatcg aatagggatc aactctccta ttgcactaat caccattttg acatttatcc
     2701 tgttgggttt gggaattctg tgttcgcagc ccgaatatgg attgatcaga ctgcttagtc
     2761 tggaagatag gtccggtatc ctggcgcgcc ggctgttgcc cgcggcgatc ggtattccca
     2821 ttttattagg ctggcttttt ctaagcgggg aacgagcagg ctattatcaa agttcatttg
     2881 gaatcgctct ctttgcttta tccagcatcc tcgtattttc agccttaatc tggcgaaata
     2941 gccgtttgct ctctacggtg gacgcagccc gtggacaggc cgaggaagca ttaagtctca
     3001 gcgaaaaacg cgcccaggct ctcattgaaa acagttggga cgcaattgcg ttgtttagcg
     3061 ccgatgggcg catcctttat ggaagtccat caacacctca agtacttgga tattcattga
     3121 gtgagtttgt aggccgcaat gcatttgaat tgatccatcc ggaggatcag gcactcgcta
     3181 ccgagcgtat gaatctttcg ctccagcagc cccgtgcaca tatccccgta aacgctcgtg
     3241 ttcttcataa aagcggggaa tggcgctggc tggaaggagt tttcacgaat ttactggatg
     3301 agccgagcat ccgggctatt gtcaacaatt atcatgactt tacggagcgc aaacgcgacg
     3361 acgatgcttt acgcgagagt gaaagttatt ctttttcttt actcagcctc tcgaagaagc
     3421 tggagcaggc ccaaacatat tccgaagccc ttaatgcggc cctcgaacag gtaaaggctc
     3481 tcctgggtta tcagaatgtc tggatgtatc tgctctctga cgataaacaa tcgctgcgcc
     3541 tgcttaccag caccggagac ttgtcgcaga agatttcaga agactttgcc actttgccga
     3601 ttaaagggga tcgttttttg gcggaaatcg ctgaagggaa ggaacctgtg ctggtcgagg
     3661 atgccctgac ggactcgcga accaataagg acattgtagg gcagttgggg aaccgcacca
     3721 ttgtgaatgt tccgattgta ctgatggaca gacatctggg tgcgtttggc acgggaagct
     3781 ttggcgaaga gggcgttcgc gttcccacca ccgtgcaact ggagtattta cgttcgttag
     3841 ccagccacat ggcagtgacg attgatcgaa ttcatctgct caccgaacgg aggcagtcgg
     3901 aagaacaaat cgcttatcag gcgtacctgc tcgaaaatgt caacgatgct gtgataggca
     3961 gtgatgaaaa tacattaatt cgattttgga atcaaggtgc cgagagaatt tttggatgga
     4021 aagcggaaga agttctcgga cggtcggctc gggaagttct tcgatcagaa atgatcaata
     4081 ctgaccggga gactgttctc aaaaaacttg cagagcaggg ccgttggcgt ggtgaagccg
     4141 ccctctatcg caaagatgga acgaaggtga ttatggaagt tagttcgatc actctgcgtg
     4201 acacgcatgg cttacttact ggctatgtaa gtgtcaatcg tgatatcaca gatcgcaaac
     4261 acatagaggc agaaatccgc aaactgaaca ccgaattgga acagcgcgtg gtggagcgaa
     4321 cggcggcgtt ttcacaggcg aacagcttgc ttcaaatgat gctggaccat atgcctgacc
     4381 agatttactt caaggacgcg cagagtcgtt ttattaggaa cagtagatcg caagcaaccg
     4441 cgcttggctt gaacgaccca gccgatattg taggcaagtc ggactttgat ttttttccac
     4501 atgcacaaca atcctttgaa attgaacagg aaatcatgcg ctcgggcaca cccatagtga
     4561 atcaggaaga gctggttcgc tggccggatg gcacagaaac ctgggtttct accaccaagg
     4621 tgccactccg tgatcaagct ggagacatcg tcggcacatt cggcatctcg cgcgatatat
     4681 ccgaacgaaa gcgggcagaa ttggccctgc agaaagccaa attggaactc gaagctgcca
     4741 acaaggaatt ggaggccttt agttattccg tctctcatga tttgcgcgcg ccgctgcgca
     4801 gcgtagacgg gttcagccag gcgattttag aggactatgg agatattctg ccggccgatg
     4861 gccgagaatt cctcgaacgc atccgtagtg ccactcaacg tatggatcga ttgattgacg
     4921 acatgttgaa cttgtcacga gtgacacgag cgccgctcaa gagcgcgccg gtaaacttaa
     4981 ccaaactggc cgaagatatt gcagctgaac ttaggcaata tgatacgaac cgcaatgtga
     5041 attttgtcat cgcgcagaac ttaagatcta acggtgatcc tcgcctgctc caaattgtgc
     5101 tggagaatct catgcgcaat gcctggaaat ttacatccaa acgtgaactg ggcgagatcg
     5161 aagtcggcgc acgagaggaa aatggcgaga ccgtttattt tattcgtgat aatggcgccg
     5221 gatttgacat gacgtatgca aacaagctgt ttggagcctt tcagcgtttg cacgcgatga
     5281 cagagtttcc gggaactggc gttgggctgg caaccatcca gcggattatt catagacatg
     5341 gtggacgcgt ttgggcggtg ggagtcgttg accagggcgc gacattttcc tttaccttgc
     5401 cgattttggc gagcccaaaa cctgtaaccc ctgtctcaga aatgaaagaa gaggacgaat
     5461 caatacaccg ccgtgccagg gaaatcatat gagtggattt atacaatgac gaccgaatcc
     5521 acgttaatct ttagcgcatt catcgcgttg actgccgcgg tcatctatgc ctatgtgggc
     5581 tggcgtttgg gcaaacgtgt tattacttct tcagagggtc ggctggcctg ggtagccttc
     5641 acgctatggt ggtatgggct ggcattaaca acattgatcg gcggtatcca gaatctgtta
     5701 ggcgcaattg gaataacgag tctgtctctc ttcgtcacgg ccacatatgt gaatattttt
     5761 ccggcctgta tcgcactatg gggactgcta tattacctgc tttacctgtt cacagggaat
     5821 agacgatcgt tattgccttt gacgctcttt tacgtcatct actatatcct gctggtctat
     5881 tacaccactg ccagcattcc cgagggtgta gaagtagggc gttggagcac cacattgatt
     5941 tatcgcacgc ccattactgg acctttcttt atcatattgg tagttttgct gtttttcccg
     6001 cagattattg gtagcatcgc atactttaca gtctacttcc gagtaacaga tccaacacag
     6061 agataccgta tcctcctggt atcgggaagt atcatggtct ggctattaag tccttatgtg
     6121 gccctggcag gtggcgtaac agaaaacgat tggtggcatc ttgcaagccg atttattggg
     6181 ttagccgctc ctctgaccgt tcttatggcg tatttgccac cctactggct gaaacagcgc
     6241 tacggcatta tttctctttc tgatgaacct aagcaatgat atataagcga ctataaacga
     6301 aataagagga acttttatgg aagaaaaaat gattctatta gtagaagata accctgatga
     6361 tgaagctcta actctgaggg ctttgaagaa acataatatt gggaataagg tttttgtcgt
     6421 gcgcgatggg gctgaggcac tggactttct tttttgtact ggaaaatatt cagaccgtga
     6481 tccaaacgat aaacctcagg tcacactttt ggatctaaag ctcccaaagg tggatgggct
     6541 cgaagtgtta cgccgcctgc gtgcggacga gcgcacccaa acgcttccta ttgtgattct
     6601 cacgtcttcg aatgaggaaa aggatttgat tcaaggttat aagaatggtg cgaattctta
     6661 tgttcgcaag cctgttgatt tcaatgaatt tcttgaggcc gtaagagatc tgggtctcta
     6721 ttggctggtc tggaactatg caccaccgcg gactaagtaa attgtcatga gaaaaccgct
     6781 gcgtgtcctg attctcgaag actctgaaga tgatagcttg ctgttgctgc gcgaactcaa
     6841 gcggagcggc tatgaggtgg aatttgagcg ggtagataca gcctcagcaa tgcgttcggc
     6901 attgacccgc aagacatggg atctgattct aagcgactat agtatgcccg atttcaatgc
     6961 tccaaaagca ttggaattgt tgaaaaccag cgaactggac ttgcccttta ttatcgtttc
     7021 aggcacgatt ggggaagaga ctgctgtcgc cgcgctaaaa gcgggtgcaa acgactttct
     7081 ggtgaaggga aaattttccc ggctgggtcc cgccatcgaa cgcgaactcc gagaggccga
     7141 atcccgaaac gatcgccgtc gcgcggaaga gcaattgcgc tatcaggcca atctccttgc
     7201 aaatgtaaca gatgcgatta ttgccgcaga cga
//
LOCUS       DASTJP010000262         4550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_868902, whole genome shotgun sequence.
ACCESSION   DASTJP010000262 DASTJP010000000
VERSION     DASTJP010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868902"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFQ23_07940"
     CDS             <1..1081
                     /locus_tag="VFQ23_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET9906556.1"
                     /translation="FDYAVVQAEDELAAIGMAVGAGWSGLRAMTSTSGPGLSLMTEFA
                     GLAYYAEVPLVVWDIQRIGPSTGLPTRTSQADLTFANAIGHGDSETIILLPGNINECF
                     DFGWQSFDIAERLQTPVFVLSDLDMGMNQWMSKPFEYPDQPMDRGKVLWEKDLEELKG
                     NWARYLDKDGDGIPYRTLPGNKHPLGSYFTRGTGHDEQARYTEDNVVFQRNMERLKKK
                     FVTAKEYLPKCVTYTTEGATIGIIAYGSTEAAINEARVQLEKDHDTKTSFLRVRATPF
                     THDVDDFVHAHDVIYVVEMNRDGQLHKLLTLAFPEAAMRLISVAFGDGMPASAKWVRE
                     GILAKANGHQPVPVAANNGSKGTNL"
     gene            1078..2133
                     /locus_tag="VFQ23_07945"
     CDS             1078..2133
                     /locus_tag="VFQ23_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET9906557.1"
                     /translation="MTQPLPMAGAPANVNLIGLTKNDYRGNPTTLCQGCGHNSISNQI
                     ITTLYELNVLPENVLKFSGIGCSSKSPTYFMNRSFGFNSLHGRMPSIATGGLFGDHTL
                     KGVAVSGDGDTASIGMGQFKHIMRRNVNMVYIIENNGVYGLTKGQFSATAERGLKLKK
                     QGSNAYMPIDLCMEAVISNATFVARSFAGNPKQVKELLKAAYSHNGLAILDIISPCVT
                     FNNNDDAHYSYGWGKEHEAPLHDITYVPPRNEIMLEKDMNAGEIREVTMHDGSVIVLK
                     NVEKQYDPTNRFEALRVLEEAQRNNWLITGLIYVATDRPTLSETYQLVETPLNRLTEA
                     DLRPAPEMLDKVNALMF"
     gene            2208..2627
                     /gene="arfB"
                     /locus_tag="VFQ23_07950"
     CDS             2208..2627
                     /gene="arfB"
                     /locus_tag="VFQ23_07950"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HET9906558.1"
                     /translation="MLQITPSIHIDERDLQIDYIRASGPGGQNVNKVATAAQLRFNIN
                     ESALPGEAKARLIRLAGKRVTGEGILIIEAKRFRTQEQNREDAIQRFSEFVRKSFETP
                     KARKKTKPTKASKETRLKQKKRKGEIKRLRNKSIYTE"
     gene            2631..3275
                     /locus_tag="VFQ23_07955"
     CDS             2631..3275
                     /locus_tag="VFQ23_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906559.1"
                     /translation="MNIDTVELIGYLGSILVAISLMMRSLLRLRIINLIGALFFTLYG
                     VLLGAYPVAFLNGLIVCIDLYYLVQMWRQKDFFTFLEVLPNSKYMKAFVEFYKDDILA
                     IIPTYTHKTAEDLLCFFILRNMMPAGLFVAKINGEEAHIELDYVIPNYRDFQVARFIF
                     EENAAFFLQRGIRRFVSDGGSPIHRTYLEKMEFVNKGDLYVHEISAKKLQDNRL"
     gene            3278..4156
                     /locus_tag="VFQ23_07960"
     CDS             3278..4156
                     /locus_tag="VFQ23_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9906560.1"
                     /translation="MNNKALIGILCGLGAAAIWGGMYVVSKVVLEIIPPFTLVSLRLL
                     LGIVTLIIVLLLQGLPKISRTEMLQVVGIGFVGYGVSLSLQFLGTKLSTAANGSLVTS
                     ATPAFVLLFASMLLKEKITTRRLVALLLATCGVIAVIDPRSAQLNPDLFLGNLLLFGA
                     AVTWALYSVLVRKMTQSLDVLPFSLVAFFGGLPVTLPAAAWELTTVGFGEISLGVVGG
                     VLFLGVISTALAMILWNTAFAVLDASAASLTFFAQPVVGTLLGWLFLGEIITPLFIVG
                     GILIGIGLVISSTEKS"
     gene            4210..>4550
                     /locus_tag="VFQ23_07965"
     CDS             4210..>4550
                     /locus_tag="VFQ23_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9906561.1"
                     /translation="MNNTQWYKDAVFYEISVRAFKDSNGDGIGDLRGLTEKLDYVRDL
                     GVDCIWIMPIYPSPLRDDGYDIADYYNVAETFGTLEDFKTLIQEAHNRNIRLIADLVL
                     NHTSDEHPWFKA"
BASE COUNT         1171 a         1079 c         1126 g         1174 t
ORIGIN      
        1 attcgattac gcggttgtcc aggcggaaga cgaattggct gccatcggta tggcggtcgg
       61 cgctggctgg agtggtttac gcgccatgac ctccacttcc gggccgggct tgagcctgat
      121 gacagaattt gcagggttgg cctattatgc agaagtgccg cttgtcgttt gggacattca
      181 acgtatcggt ccctcgactg gtttaccgac ccgcacatcc caggcagatc tcacctttgc
      241 aaatgccatt gggcacggtg acagcgagac catcattctg ctgccaggca acatcaatga
      301 atgttttgat ttcggctggc aatcgttcga tattgccgag cgtttacaga cgccagtttt
      361 tgttctgtca gacctggata tgggcatgaa tcagtggatg agcaagccgt ttgaatatcc
      421 agatcagcct atggaccgcg gcaaagtttt atgggaaaaa gatcttgaag aattgaaggg
      481 gaattgggct cgttatctcg acaaagatgg cgatggcatt ccctatcgca ccttgcccgg
      541 caacaaacac cccctgggtt cctatttcac acgtggaacc ggtcacgacg aacaggcccg
      601 ttatacagaa gataatgttg tgttccagcg caacatggaa agactcaaga agaagtttgt
      661 tacagccaaa gagtatttgc caaagtgtgt tacctatacg acagagggtg caacgatcgg
      721 catcattgct tatggatcaa ccgaagcagc catcaacgaa gcccgcgttc aattggaaaa
      781 agaccacgac acgaaaacca gctttttacg agtacgcgcc actcccttca ctcacgatgt
      841 ggatgatttc gtccatgcgc atgatgtgat ctacgtggtg gaaatgaatc gtgatggtca
      901 actgcataag ttgttgacgc ttgctttccc tgaagctgcc atgcgcttga tctcggttgc
      961 ttttggagat ggcatgccag cctcggcaaa gtgggtgcgt gagggaatcc tcgcaaaagc
     1021 caatggacac caacccgtac ctgtcgctgc aaacaatgga tcgaaaggaa ccaacctatg
     1081 actcaacctc ttcccatggc tggtgcgcca gcaaatgtaa acctgatcgg tcttacaaaa
     1141 aacgattacc gcggcaaccc cacaacgctg tgccagggct gcgggcacaa ctcgatttcg
     1201 aatcaaatta tcactaccct gtatgagctg aacgtgctgc ccgaaaatgt tcttaagttt
     1261 agtggcattg gctgctcgag caaaagcccc acctacttca tgaaccgctc ctttgggttc
     1321 aacagcctgc atggacggat gccatccatt gccaccggtg gtttgtttgg cgatcacacc
     1381 ctgaaaggtg ttgccgtctc cggagatgga gataccgcca gcattggtat ggggcagttc
     1441 aagcacatta tgcggcgcaa cgtcaatatg gtttatatca tcgagaacaa cggtgtatac
     1501 ggcctcacta aaggacaatt ttccgcgacg gcggaacgag ggctcaagct taagaagcag
     1561 ggctcgaatg cgtatatgcc gattgatttg tgcatggaag ccgtcatttc gaatgctacc
     1621 tttgtcgccc gctcctttgc cggtaacccc aaacaagtaa aggaattgct gaaagccgcg
     1681 tattcacata atgggctagc gatcctcgat atcatcagcc cgtgtgtcac gttcaacaat
     1741 aatgacgatg ctcattattc ctatggatgg ggcaaagagc atgaggcacc gcttcatgac
     1801 attacctacg ttccgccgcg caatgagatc atgcttgaaa aggacatgaa cgcgggcgag
     1861 attcgcgaag tcaccatgca cgatggatct gtaatcgtcc ttaagaacgt agaaaaacaa
     1921 tacgatccga ccaatcgttt cgaagcctta cgtgtgctcg aagaggcgca gcgaaataac
     1981 tggctcatta ccgggttaat ctacgttgcg acagatcgcc ccacgctgtc agaaacctac
     2041 caattagtag aaacacccct caaccgcctc accgaagcag atctccggcc tgcccctgag
     2101 atgctggata aagtcaacgc tttgatgttt tgaccccctc ccgcctttgg caccctcccc
     2161 cattttctga agtacgaaaa tgggggaggg gtgggggctt tttttttatg ctccaaatta
     2221 ctccttcaat acatattgac gaacgagatt tgcagataga ctatatccgc gcctctggcc
     2281 caggcgggca aaatgtcaac aaggtagcga cggctgcgca gttgcgattc aacataaatg
     2341 agtcagctct gcctggagaa gcaaaagcac gtttgatccg attggcaggc aagcgcgtga
     2401 caggcgaagg catactaatt atcgaagcga agcgatttcg cacgcaggaa caaaaccggg
     2461 aagacgcgat ccaaagattt tctgaatttg taagaaaatc ttttgaaacg ccaaaggcgc
     2521 gcaagaaaac caaaccgact aaagcatcga aagaaacaag gttgaagcag aaaaaaagaa
     2581 aaggggagat caagcgcctg cggaacaaat ctatctacac ggaataagac atgaacatcg
     2641 acactgttga acttatcggt tatcttggct caattctggt ggcaatttca ctgatgatga
     2701 gatcgctcct gaggctgcgt attatcaatc tgattggcgc tctattcttt acgctgtatg
     2761 gtgttctgct cggtgcctac ccggtagcct tcctcaatgg gctgattgtt tgcatcgatc
     2821 tttattatct cgtccagatg tggcggcaaa aagacttttt tacctttctc gaggttttac
     2881 cgaacagcaa atatatgaag gcctttgtgg aattttataa ggatgacatc ctggcgatta
     2941 tcccgaccta cacccataaa acagcggaag atttactctg ttttttcatc ctacggaata
     3001 tgatgcctgc cgggctgttc gttgcgaaaa ttaatggaga agaagcacac atcgagcttg
     3061 attatgtcat tccaaactat cgcgactttc aagtagcacg ttttatcttt gaagaaaatg
     3121 cagcgttctt ccttcagcgt ggaatccgtc gctttgtttc ggacggcgga agccctattc
     3181 accggacgta tttggaaaaa atggaatttg taaataaagg ggatttgtat gttcacgaga
     3241 tcagcgcgaa aaagcttcag gataacagac tttaaaattg aacaacaaag ctttgattgg
     3301 aatcctttgc gggcttggtg ccgcagcgat ctggggtggg atgtatgtag tcagcaaagt
     3361 tgttcttgag atcatcccgc cgtttacgtt agtctccctc cgtcttttgc ttggcatcgt
     3421 caccctcata atcgttcttt tattacaagg tttaccgaaa atttctcgta cagaaatgct
     3481 gcaggtcgtt gggatcgggt ttgttggcta cggggtttca ttaagtttac aatttctcgg
     3541 aacaaaatta tctacagcgg caaacggttc cctggtgaca tctgccactc cggcctttgt
     3601 ccttttgttt gcgtcgatgc tgctgaaaga aaaaatcaca acacgaagac tggttgcact
     3661 tcttctcgct acatgtgggg tgatcgctgt gatcgatccg cgctctgcac agctcaaccc
     3721 ggatctgttt cttggaaatc tattgttgtt cggcgccgcc gtcacctggg ctttgtattc
     3781 tgtattagtt cgcaaaatga cccaaagcct ggatgtgctg ccatttagcc tggtcgcatt
     3841 ctttggcgga ttgccagtaa ctctcccggc tgccgcgtgg gagttgacga cagttgggtt
     3901 cggggaaatt agccttgggg tcgttggagg tgtgctgttt ttaggagtca tatccactgc
     3961 cctagcaatg atcttatgga acacggcatt tgcggttctg gatgcaagcg cagcttcgct
     4021 gactttcttt gcccaacccg ttgttggaac cttgttgggc tggttatttt taggagaaat
     4081 aattacgcct ttgttcattg tgggggggat actaatcgga attggacttg taatttcaag
     4141 cactgagaaa tcataaattt tttcgacatt tagaagagca ttaatgaaaa atcaagttac
     4201 aatcggctca tgaataatac acaatggtat aaagacgcgg tcttttatga aatctccgtg
     4261 cgcgccttca aagacagcaa tggcgatggc attggcgatt tacgtggact taccgagaag
     4321 ctggattatg tgcgggacct gggagtggac tgcatttgga tcatgccgat ctatccttct
     4381 ccgctgagag atgatggtta cgacatcgcc gactactaca acgtggcgga gacgtttggc
     4441 acactggaag atttcaaaac cctgattcaa gaagcgcata atcgaaatat ccggctcatc
     4501 gcggacctcg tgttgaatca cacatccgat gagcatccct ggttcaaagc
//
LOCUS       DASTJP010000263         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1195774, whole genome shotgun sequence.
ACCESSION   DASTJP010000263 DASTJP010000000
VERSION     DASTJP010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195774"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFQ23_07970"
     CDS             <1..369
                     /locus_tag="VFQ23_07970"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET9906562.1"
                     /translation="YHVPFDRICMHEFVAEGRFKGSDVRALDIAKRLMDYNFHPPTNY
                     FPLIVHEALMIEPTETENKDTLDAFVDALIKIAEEAKSEPELLHNAPSTTQFGRMDEV
                     KAARELVLCCWLPEGYDLNQ"
     gene            490..1329
                     /locus_tag="VFQ23_07975"
     CDS             490..1329
                     /locus_tag="VFQ23_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9906563.1"
                     /translation="MKQTIIRILPKWVKVLLKKMYYLLFDLFDTSTKRNSMIPPRSMT
                     FIGGGDFIKVGEGFKKHFVELGDLKPTDRVLDVGCGIGRMAIPLTSYLSEQGEYWGFD
                     IVKAGIDWGQKRISSKFGNFHFLHSDVYNKHYNPEGRIQARDYRFPFESGFFDFVFLT
                     SVFTHMLPPDVENYMSEISRVLKPGGKCLITFFILNEESVRLVRSGKSRLNFKYPLDG
                     CVTIDKNTPEEAIAYEEEVVRNFFDGNGLVISQPIYYGSWCNRQKFVTYQDLIVAEKR
                     RLI"
     gene            1353..1844
                     /locus_tag="VFQ23_07980"
     CDS             1353..1844
                     /locus_tag="VFQ23_07980"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HET9906564.1"
                     /translation="MTDPLKTSGKILSLLEGTWAGEGRGDFPGVSSFDYRERLTFTRR
                     DEKTLAYEQRAQKRYDGQTEYLQSHWESGFLSSLENDDLQLVNIQLGGRSEVLVGVIE
                     SLDTLFRIHFVSKTLNNDPRMLSSTRTFELEGDILRYEMAMHTTKVERLTPHLKITLQ
                     RAR"
     gene            1923..>2290
                     /locus_tag="VFQ23_07985"
     CDS             1923..>2290
                     /locus_tag="VFQ23_07985"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET9906565.1"
                     /translation="MTTLMTIPKTQYPSMDDARLAQQARTDPQAFAELYRRHVRSIYR
                     YQLAHTGNVRDAEDLTSQTFMAALEGIRSFRGTGPYITWLIGIASRKRALFFRRSGNE
                     PEVPLEAALHLPTTGLPTDKA"
BASE COUNT          593 a          539 c          590 g          568 t
ORIGIN      
        1 tatcatgtcc cgtttgaccg catctgcatg cacgagtttg ttgccgaagg gcggtttaag
       61 ggaagtgacg tgcgcgccct ggatatcgcc aagcgtttga tggactacaa ctttcatccg
      121 cccacaaact acttcccgct catcgtgcat gaagcactga tgatcgagcc aacggaaacc
      181 gagaacaagg acacgctcga tgccttcgtg gatgcgctga tcaagatcgc cgaggaagcc
      241 aaatctgagc ccgagctgct ccacaatgcc ccaagcacca cgcagttcgg caggatggac
      301 gaggtcaagg cggcgagaga gttggtcctt tgctgctggc tgccggaagg atatgatttg
      361 aatcagtaag ccagtggcgg ttattcggaa aacacgaaag ccccagagag aatttgctcc
      421 ggggcttttg gtttctctac tctataatta acttatgctg gacagaaaac caagctggag
      481 aatttcgcag tgaagcaaac aataatcagg atactcccca aatgggtcaa agtactgctg
      541 aaaaagatgt attatcttct tttcgattta tttgacacgt ccacgaaacg aaatagcatg
      601 attccgcccc gctccatgac ctttattggt ggtggtgatt ttattaaagt gggagaaggg
      661 tttaagaaac attttgttga attgggggat ttaaaaccaa ccgatagagt tttggatgtc
      721 ggctgcggaa ttgggcgaat ggcaattccg ttaacaagct atctatcgga acagggtgaa
      781 tactggggat ttgatatcgt caaagccgga attgattggg gtcaaaaacg tatttcttca
      841 aagtttggca atttccactt tttgcacagt gatgtataca ataagcacta caatccagag
      901 ggaagaattc aagcccggga ttatcggttt ccatttgaga gcggattttt tgatttcgtt
      961 tttctgacgt cagtattcac gcacatgctg ccaccagatg tggaaaatta tatgagtgag
     1021 atttctcgag tcctcaagcc gggcggaaaa tgcctgatca cgtttttcat tttgaatgaa
     1081 gaatccgtgc gtcttgtgcg ttccggaaaa agcaggctta acttcaaata ccctttggat
     1141 ggctgcgtaa ccatcgataa gaacacgccg gaagaagcga ttgcctatga ggaagaagtt
     1201 gtcaggaatt tcttcgatgg gaatgggctg gtgatcagcc agcccatcta ttatggttcc
     1261 tggtgtaaca ggcagaaatt tgttacctat caggacctca tcgtagctga gaaaagacgt
     1321 ttgatttagg tgcttttcat aataagacag tcatgacgga tccattaaaa accagcggca
     1381 aaatactctc gctcctcgaa ggaacctggg cgggcgaggg gcgcggagat ttccccggtg
     1441 ttagttcctt tgattatcgt gagaggctta ctttcacacg gcgtgacgag aaaactctgg
     1501 cctatgaaca gcgggctcaa aagcgatacg acgggcagac agaatatctc caatcgcatt
     1561 gggagagtgg tttcctaagc agcctcgaaa acgatgattt gcaattggtc aacatccaat
     1621 taggcggccg aagtgaagtg ttggttggtg ttatagaatc gcttgacaca ctcttccgga
     1681 tccattttgt tagcaaaact ctgaataacg acccgcgcat gctttcctcc acacgcacct
     1741 ttgagctgga aggcgatatt ctacgttacg aaatggcaat gcatacaaca aaagtggaac
     1801 ggttgacgcc gcatctgaag atcacgctgc agcgtgccag gtaataattc ctcgatattc
     1861 cctgttacga ttcgggggtc tcctgtaacc tacttacgtg tacataccct ttcaggtgat
     1921 gcatgacgac ccttatgacc ataccgaaaa cccaatatcc gtccatggac gatgcccggc
     1981 tggctcaaca ggcccgcaca gatccgcagg cgtttgctga gctgtaccgg cgccacgtcc
     2041 gcagtattta tcgctatcaa ctggcccaca cgggtaatgt cagggatgcg gaagacctga
     2101 cttcacagac gttcatggca gcgctggagg gcattcgctc gtttcgcggc acgggtccgt
     2161 atattacgtg gctgatcggg atcgcctcgc ggaagcgtgc cctgttcttc cgaaggagcg
     2221 ggaatgagcc tgaggtcccg ttggaggcgg ctcttcacct ccccaccaca ggcctgccca
     2281 ccgacaaagc
//
LOCUS       DASTJP010000264         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_913638, whole genome shotgun sequence.
ACCESSION   DASTJP010000264 DASTJP010000000
VERSION     DASTJP010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913638"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFQ23_07990"
     CDS             complement(<1..327)
                     /locus_tag="VFQ23_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906566.1"
                     /translation="MEATTHYYLSKVITILFWWINLFGLYFIFGGLYVGFSSWFTSIV
                     GNLLGFMFFFVFGLFILFFGNMFPEIITNEDGLFIRFLFWRLKVKWDDLEEIKEQTLS
                     RFLTGSS"
     gene            complement(333..1370)
                     /gene="hrcA"
                     /locus_tag="VFQ23_07995"
     CDS             complement(333..1370)
                     /gene="hrcA"
                     /locus_tag="VFQ23_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET9906567.1"
                     /translation="MQELSERQKTLLLLIIRDYIESAQPVGSKRLAEHYHISLSTATI
                     RNEMGALTEMGYLRQPHTSAGRVPTEEGYRYFVSQMMHNAELPETIQRTISHQFHQSR
                     ADMNQWMTLAASVLAHQSQGVSVVTAPHAEQSRYKHVELISTQGRQVLMVLVMVGGEV
                     SQQILTLAEPVSQERLSQTAARLNGLLAGLAADSIASLPTRSDALDQDILTLILQDMR
                     RTANQDSGEIFTDGLTNVLSEPEFSDSDEARRALKLFEERSTLRNLLARTATNSNIGG
                     LQVLIGGEGEWEELKQCSIVLARYGVPGMATGTLGVLGPMRMSYAKTIPTVRYVAGLL
                     SDLVNDTISGE"
     gene            complement(1521..>2567)
                     /locus_tag="VFQ23_08000"
     CDS             complement(1521..>2567)
                     /locus_tag="VFQ23_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906568.1"
                     /translation="STPLPLPTETPLSLETPTAVSATALVEVTATHTLEPTATLRPAV
                     QISPTPGDTTLVATPLGGSSGQIAFSSARSGIPQIYLMNTDGSGLTLLTNMEEGACQP
                     TWSSNGDRLAFISPCRERGDFAGVVYPDSSVYIMNADGSGATKLTTGPEANFDPAWAP
                     DGNRVAFTSVRDGYKQIYMINLDSQVETRLTNTSSEIEASQPAWSPFGNQITYLVKRL
                     GVYQIWTMSDDGQDSEQLVRSGQNLWDFLPTWSPDGSFILFSQRSPNPTRPWLMRVNV
                     ANANQGPSRLNFPTPIEDVEFSPDGIWLVFEGMDSEANREIYLITITGGSLTRLTNEP
                     NVDFDPAWRPIQNP"
BASE COUNT          703 a          624 c          618 g          622 t
ORIGIN      
        1 cgaacttccc gttagaaatc ttgataaggt ttgttcctta atttcttcca ggtcatccca
       61 ctttactttc aagcgccaaa aaagaaatcg aataaataat ccgtcttcgt tagtaattat
      121 ttcaggaaac atatttccaa aaaacagaat aaacaaaccg aaaacaaaaa agaacatgaa
      181 accaagcaga tttccaacta tgcttgtaaa ccaggaacta aagccgacgt aaaggccacc
      241 aaatataaaa tatagtccga acaggttgat ccaccaaaac aaaatcgtaa tcacctttga
      301 caaataataa tgcgtagttg cttccatatg aattattcac cagaaatggt gtcattgaca
      361 aggtcactta acaaccccgc cacatatcgc acagttggaa tcgtcttcgc atatgacatt
      421 cgcatgggac ccagtacgcc cagtgttccc gttgccatac ctgggacgcc ataacgcgcc
      481 aggacgatgg aacattgctt cagttcttcc cattctcctt cgccaccgat tagcacctgc
      541 agccctccga tattactatt tgtggcggtg cgagcaagca ggtttcgcaa ggtggatcgt
      601 tcctcaaaaa gctttaatgc acggcgagct tcgtccgaat ccgagaattc aggctcggat
      661 agaacgttcg tcaagccatc ggtaaaaatc tctcccgagt cctggttggc agtacgacgc
      721 atatcctgca aaatgagggt cagaatatcc tggtcaagcg catcggaacg cgtcgggagt
      781 gacgcgattg aatccgctgc caacccagcc aaaagaccgt taagacgcgc agccgtctgg
      841 ctgagtcgtt cctgtgagac aggttctgcc agagtgagga tctgctggct gacttctccg
      901 ccaaccatca ccagaaccat aagtacctgg cgcccctgcg tggagatcaa ttccacatgc
      961 ttgtagcgcg attgttcggc atgcggagcc gtcacgacgg aaacaccctg ggactgatga
     1021 gcaaggaccg aagccgcaag cgtcatccat tgattcatgt ccgcgcgtga ttgatgaaac
     1081 tgatgcgaaa tcgtccgttg aatcgtttca ggtaactcgg cgttgtgcat catctggcta
     1141 acaaaatagc gatagccttc ttctgtgggc acacgccctg cagaggtgtg aggttgacgc
     1201 aaatagccca tctctgtcag cgcgcccatt tcattgcgaa tcgtggccgt tgaaagactg
     1261 atatgataat gctccgccag ccgcttcgaa cccacaggtt gagcagattc aatgtaatcg
     1321 cggatgatta gtaaaagaag ggtcttttgt cgttctgata attcttgcat gatcttcaca
     1381 taattagcac tctcgcgttg agagtgctat tcaaaggtag ataatcataa catgcgtaaa
     1441 agaaggacgt caataagagg aacgtaagaa aatgcccatt cctttgtaag gaatgggcat
     1501 gaggttatta ccgtattgat ttatggattt tgtatgggcc gccaggcagg gtcaaaatcg
     1561 acgttaggct cattggtaag gcgcgtgaga ctaccacctg taatcgttat gagataaatt
     1621 tctcgattgg cttcgctatc catgccttca aagaccaacc atattccgtc cggagaaaac
     1681 tctacgtctt cgatgggtgt cgggaagttc aaacgtgaag gtccctgatt cgcattggca
     1741 acattcactc tcattagcca gggtcgggta gggttgggac ttcgctgact aaagagaata
     1801 aacgaaccat ccggagacca ggtaggcaaa aagtcccaca aattttggcc actgcgcaca
     1861 agttgttcac tgtcttgacc atcatcagac atcgtccaaa tttgatagac gccgagccgc
     1921 ttcacaaggt atgtgatctg gtttccaaat ggtgaccagg caggctggct cgcttcgatt
     1981 tcagaagatg tgttcgttaa acgtgtctcc acttgtgaat cgaggtttat catgtaaatt
     2041 tgcttgtatc cgtctcgaac cgaagtgaag gcgacgcggt ttccatcagg cgcccaggcg
     2101 gggtcaaaat tcgcttcagg tcctgtggtc aactttgttg cgcctgaccc atccgcgttc
     2161 ataatgtaca cactggaatc cggatagacg acaccagcaa aatcaccccg ttcacggcac
     2221 ggagaaataa atgccaatcg atcaccattg gaagaccacg ttggctgaca ggccccttcc
     2281 tccatattgg tgagcaaagt taatccgctg ccatccgtat tcattagata gatttgaggt
     2341 atcccggaac gtgcagagga aaatgcaatc tgaccggagc ttccacctaa aggagttgct
     2401 actaatgtcg tatcaccggg cgtgggagaa atttgaacag caggtcgcag cgtggcagta
     2461 ggttcgagtg tgtgggtagc tgtcacctcc accaaagccg tcgcagaaac agcagttggt
     2521 gtttccagag acaggggagt ttcagtggga aggggaagcg gcgttga
//
LOCUS       DASTJP010000265        11040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1485031, whole genome shotgun sequence.
ACCESSION   DASTJP010000265 DASTJP010000000
VERSION     DASTJP010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485031"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VFQ23_08005"
     CDS             complement(<1..493)
                     /locus_tag="VFQ23_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9906569.1"
                     /translation="MRVLLIDDEQFYFKLIRTKLREANYDLDYAKSGREGLAKIPSFE
                     PEMLIVDLKLPEMDGFEILERLRRDPKFSHIPVIVITSKDELSEKLKAFELGADDYVV
                     KPFQPEELVARMGILARRGKAMQIVQQMEKTSSRNNATIVTVHSLRGGLGCSSIVVNL
                     GLAL"
     gene            complement(526..1347)
                     /locus_tag="VFQ23_08010"
     CDS             complement(526..1347)
                     /locus_tag="VFQ23_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9906570.1"
                     /translation="MNISTADQPDLSNSPRLAILVVDDDELSRRMMKLLLSREGHRVE
                     LAPNGFEAIEIIKSQKFDIVFMDLQMPGMDGIETSQKIRKWENGGMRTFIVALTASYL
                     PEQGQELFDAGMDNYLSKPFELEHIQRMLKYRSEAILAAASHAEVLHPEEISISEVLD
                     FKKGVQRIGGDIEAFWELLDGFIQELPEKLEALQGFFLAGDMEGLSRGAHNLKGVTAN
                     LGILQLSEYADRLDKQSSAGYTDRIEYTLKEIRAIGGRLIELAQLFLSGRKDRAE"
     gene            2212..3975
                     /gene="aspS"
                     /locus_tag="VFQ23_08015"
     CDS             2212..3975
                     /gene="aspS"
                     /locus_tag="VFQ23_08015"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET9906571.1"
                     /translation="MYKTHTCGELRVTHAGQHVTLAGWVHRRRDHGGVIFLDLRDRYG
                     IIQITINPNLSKETLDKVSNVRMEWVLQITGTVHKRPAGMENPKMQTGEIEIIAESIE
                     ILNPAKTLPFMVSGENDLPDENTRLKYRYLDLRRERMARNMVLRHKVVKFMRDFLDKQ
                     NFIEIETPILFKATPEGARDYLVPSRIYPGQFYALPQSPQQLKQLLMVAGMDRYFQIA
                     RCFRDEDSRGDRQPEFTQLDLEMSYVHRDDVLTLVEALYTEMIPAVAPHKKLLSSPWP
                     KFSYREVVERFGTDKPDLRFGMELIDVSDVFAKSEFKVFQSALEAGGVVKCIVAPKSA
                     NMSRKEVDALTESAKALGAKGLATLAVTSEGLKGTAAKFVKPEEVDAVKSRTNAQEGD
                     LILFAADQRVVVNKVLGGLRLIFRDELDLADPTIMAFAWVVDFPMFAWNEEEKKWDAE
                     HHPFTMPQLDDLAKFETNPAEILSDAYDMVCNGYETASGSIRIHRSDIQLRVFQLLGL
                     SGDEIENKFGHMLEAFEFGAPPHGGMAPGIDRLIMLLADEPNIREVIAFPKNQTGRDV
                     MADAPSEVEPRQLKDLHIKIA"
     gene            3997..4218
                     /locus_tag="VFQ23_08020"
     CDS             3997..4218
                     /locus_tag="VFQ23_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906572.1"
                     /translation="MNLKELIINKTAKFAYCTDGALWYEVDGFRFPVPFEDTVGAYFV
                     PEHKAINLMRWIRKQLEEYEAQKSIRQAN"
     gene            4230..4574
                     /locus_tag="VFQ23_08025"
     CDS             4230..4574
                     /locus_tag="VFQ23_08025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET9906573.1"
                     /translation="MNVKPGDVFWISPKESNGIASDHTHPYVVIHDGPINTVVVCALT
                     SNLKRAKEPGNVLLEEGEANLPKQSVVVVSKEFTLDTIMLGQYIGTVNEQRINQVLAG
                     LRFLKSMIKHHA"
     gene            4614..4925
                     /gene="gatC"
                     /locus_tag="VFQ23_08030"
     CDS             4614..4925
                     /gene="gatC"
                     /locus_tag="VFQ23_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET9906574.1"
                     /translation="MSKKQEVQVDEKLVREIASLARLDLTAEETATFATQFKDILEYV
                     NILDEVETESVRPAYMSSANKSVTREDEIEESVPTEEFLANAPLSKDDYVLIPRVHIE
                     Q"
     gene            4928..6400
                     /gene="gatA"
                     /locus_tag="VFQ23_08035"
     CDS             4928..6400
                     /gene="gatA"
                     /locus_tag="VFQ23_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET9906575.1"
                     /translation="MDLHELSIQQASELLSSRKISSEELTRAYLERIHRLDSQVKSYV
                     TVSEELALEQAREADKRIKIGKGLTPLTGIPYSAKDSISTRGVNTTCSSKILENYKPF
                     YDSTAISKLNATYSVLLGKNNMDEFGMGSSTENSGFFTTRNPWNYDYVPGGSSGGSAA
                     AVAAGLTPFSLGEDTGGSVRMPASFCGITGLKTTYGRVSRYGLIPLASSFDTIGPMAR
                     SVYDVALVLETIGGHDPKDSTSRIEPVQRYSETLDKADNLRGLRIGIPKEYFVEGLDS
                     AVDFSVREAIRQIQGMGAEVVEISLPHTRYAIPVYYLILFAEASSNLAKYDGVRFGLS
                     ESNVDNLLDVYLKTREAGFGAETKRRIMLGTFALSAGYYDAYYLKAQKVRTLIRQDFQ
                     KAFETCDALITPVSPTTAFRIGEKVTNPLDMYLSDVFVVAVNLAGIPALSVPCGISNG
                     LPIGMQIMGPHLSEETLLHIGHMYQAQTDWHQKHPPCWNE"
     gene            6577..8019
                     /gene="gatB"
                     /locus_tag="VFQ23_08040"
     CDS             6577..8019
                     /gene="gatB"
                     /locus_tag="VFQ23_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET9906576.1"
                     /translation="MKYEPIIGLEVHAELLTQSKMFCGCEVVDSITAKPNRYICPVCT
                     GQPGALPVLNKKAIELAVRVGLALNCEIHTESIFARKNYFYPDLPKGYQISQYDQPLA
                     TNGKLEIETEAGIKEIRIRRVHLEEDTGKLSHADGHSFIDYNRSGVPLLEIVTEPDLR
                     SPDEAKAYATKLHAILRYLDVNSGDMEKGVIRFESNVSVREEGSDQFNTRTEIKNLNS
                     FRALTQGTEYEIKRQTEIYSRGGTVEQETLGFNESTGKTYSQRGKESAHDYRYFPEPD
                     LPPLVLDSNWIESIRASLPELHEAKILRFIERYELKAQDARLLTSERALADYFESVVA
                     KSKSSPKSVSSWIAGELMRHLNDSGIGIENITLSPESFARLIDMTTDKVISGNSAKVV
                     FNEMLKNGGDPEQIVKDKGLAQVSDEGFVQEAVMKVLNDNPSEVEKYLAGKDTLLQWF
                     MGQVARATKGKADPILTKKILLKALESQKT"
     gene            complement(8052..9797)
                     /locus_tag="VFQ23_08045"
     CDS             complement(8052..9797)
                     /locus_tag="VFQ23_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906577.1"
                     /translation="MITQLKKKIDANFYFSNILVPVIVLVCYSGSFAYFSSRFLLKGV
                     SYFFASSLAIYGLFLLVAVALIFLVMLKIRTGGKLAFKPSLEKINREDFLLLLLPLTP
                     VVQYVLHNQDLLSVVDSVYVLIFFLFFSSFYIFAIPAVLSSVISTRTLMVLGLAFVFT
                     ITSMASISDYFAWFEMGSLTIQVTVFGFVFVISWFLYKSNQRRVLYSFIVVNFIASGV
                     APLLSQDARVDYSPVPREENVLLSIVAERMPAMTPNIYLLIYDSYVPNETMLAYGIDN
                     GAQESYLSQQGFELYPHTYSINASSVETMSRVLNASTEYYGDLRRAVSGDGITHTILK
                     EAGYKTYGLFSSDYMFLGYGESYDYSSPERSIPPYIQLLKAILIGEFRFNIETIGFNK
                     VPRAEFLDTKQSIFADRPRNQFFVYMHTDLPSHSQNSGACLFNEVDLFEERLRRANLE
                     MKQDINLIIENDPGAIVIVAGDHGPYLTKNCHVTTGVYDISEISRLDIQDRYGTFLAI
                     RWPTDDFVLYDDITVLQDLFPAVFAYLYEDTSILKSKIEPIIVIPNEVSGASVNGGVI
                     IGGMDDGESLYLSDE"
     gene            complement(10019..10549)
                     /locus_tag="VFQ23_08050"
     CDS             complement(10019..10549)
                     /locus_tag="VFQ23_08050"
                     /inference="COORDINATES: protein motif:HMM:NF016413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET9906578.1"
                     /translation="MSNRYKIRQNLISIGDDFWIENAEGKRVFKVDGKVLRIRKTLVF
                     EDLNGRKLAQIQERLLAIKDTMAIQDADGDQIATVRKALIAPLRDRWSVNVKDGPDLD
                     VRGNIFDFEYAIMQGRNQVAQVSKQWFTFTDTYGVEIAPGQNDILILSVAIAIDMMAH
                     DDDGKKRKDRKDRKER"
     gene            10781..>11040
                     /locus_tag="VFQ23_08055"
     CDS             10781..>11040
                     /locus_tag="VFQ23_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906579.1"
                     /translation="MDEQTFNNLRLRLGDEHLGKRMRAQVDHIINVTGQGLGSFHYEN
                     VPLFVRVVDLALRVSGLKKYGQKQALKFVVRENQVPFPDLPKS"
BASE COUNT         3079 a         2560 c         2536 g         2865 t
ORIGIN      
        1 ggagtgccaa acccaaattg acaacaatac tcgagcaccc taacccacct cgcaaactgt
       61 gcactgtaac aatggtggcg ttgtttcggc tgcttgtttt ctccatctgc tgaacgatct
      121 gcattgcctt gccgcggcgc gccaaaatac ccattcgggc aaccagctct tccggctgaa
      181 atggctttac aacgtagtca tcggccccca actcaaatgc cttcagtttt tcgcttagtt
      241 catccttcga tgtaataacg ataactggaa tatggctgaa cttgggatcc cggcgtaagc
      301 gctctagaat ctcaaaacca tccatttctg gtagcttgag atcaacgata agcatttcag
      361 gttcaaagga tggaatttta gccaatcctt cacgacctga ttttgcatag tcaagatcgt
      421 aattagcctc cctcagtttc gttcgaatta acttaaagta gaactgttcg tcgtcgatca
      481 ataagacccg catttgctgc tcctggccaa taggaatggg ttggattact ctgcacgatc
      541 ttttctacca gacaggaaaa gttgcgccag ttcgattaac cttcctccaa tggctctaat
      601 ctctttcaac gtatactcaa ttcgatcagt atatcccgcg ctactttgct tatcaagcct
      661 atcagcatat tcagataatt gcaagattcc caaattggcg gtgacgccct tgaggttatg
      721 ggcaccacga gaaaggcctt ccatatctcc tgccaagaaa aatccttgca aggcttccaa
      781 tttttctgga agctcctgaa tgaagccatc cagcaattcc cagaaagctt cgatgtcgcc
      841 cccaatgcgc tgcactccct tcttaaaatc aagcacctcg ctgatcgaga tttcttctgg
      901 atgcagtact tccgcatggg aggcagcggc aaggatggct tctgacctat attttagcat
      961 gcgctgaatg tgttcgagct cgaacggctt ggaaagatag ttatccatgc ctgcatcaaa
     1021 caactcttgt ccctgctcag gtaaataact tgctgtcaac gccacgataa acgtgcgcat
     1081 cccgccattt tcccacttac gaatcttctg actggtctcg ataccatcca tgcctggcat
     1141 ttgaagatcc atgaatacga tgtcaaactt ttgagactta atgatttcaa tcgcctcaaa
     1201 accattaggc gctagctcta ctcgatgacc ttcacgtgaa agtaataact tcatcattcg
     1261 tcggcttaat tcgtcatcgt caacgacaag gatagctagc cgcggagaat ttgaaagatc
     1321 tggctgatcc gctgtcgaaa tattcatgct caaaactatc tcctgactta gaggcggcaa
     1381 ttcggccctg caccgaaaac aaggtctgaa aaattcaata cgaagtatgc atttgaaagt
     1441 aacgatgcca cacgttaatg agggtgctag actagctccc gccaagttca tgacgccaaa
     1501 taagtctgaa ggttacccag gcaatcgcct gtttcacatg tgcaatgaaa tttcaacagg
     1561 tgcctgtgaa atctttttta taatagccca tgggactttt gctattggtt ggtaagtgtg
     1621 gaatatacaa catctgcccg cagtttatcc ttacactcgc tttacattct gtttttttat
     1681 cattcgtgga aaatcagaat gggggactct caccgatttc ggctgtgata cttgctcaga
     1741 cgattttgcc aggccatttc gattctagga gaaagtcagt tttgctgatc tccttgaatt
     1801 taaccctgga cttctcctgg tggacgtgac aattgtgatg atcgtggcca gaataggcgg
     1861 ttggtagtcg cctcacgagc gaaggcagaa ttcagagaaa tttagtcgat aagaatgagt
     1921 ctatgctata atataaatac gatgaggttt tgtcaattgg gctttgccaa taatcatgac
     1981 aacgataata acgatcttga aatcgttggg cgaagctttg ctggttgaaa agtaagttct
     2041 gaacagaaac caatcgccca acgcaatagc gaagggcgat ttattttatc tataccgtgt
     2101 cattgcgatg aggcccatca gggatgacga agcaatcccc taattaaaca ggagattgct
     2161 tcgtcgcaac aaacgcttct cgcaatgaca tttaatcaac ggagacacac tatgtataaa
     2221 actcatacct gtggtgaatt gagagtcact catgcaggtc agcatgtgac tttggctggc
     2281 tgggtccatc gacgtcgtga tcacggcggc gtcatttttc ttgatttgcg tgatcgttat
     2341 ggaattattc aaataaccat caatcccaat ctttccaaag agactctcga taaggtatca
     2401 aatgtccgca tggaatgggt tcttcagatc acaggcacag tccacaagcg ccccgccgga
     2461 atggaaaacc ccaaaatgca aaccggtgaa attgaaatca ttgccgagtc aattgagatt
     2521 ttgaatcccg caaagacgct gcccttcatg gtcagcggtg agaacgacct gccagatgaa
     2581 aacacacgcc tcaaatatcg ttatcttgat ttgcgtcgtg agcgtatggc tcgaaatatg
     2641 gtcttgcgac ataaagtcgt taagttcatg cgcgattttc tcgacaaaca aaacttcatt
     2701 gagatcgaaa caccaatcct tttcaaggcc acccccgaag gtgcgcgcga ttatcttgtc
     2761 ccatcacgta tctacccggg acaattctac gccttgcctc agtcaccgca acaactcaaa
     2821 cagttgttaa tggtcgcggg catggaccgt tacttccaga tcgcccgttg tttccgcgat
     2881 gaagactcac gcggcgaccg tcagccggag ttcacccaac ttgaccttga aatgtcctat
     2941 gttcatcgag acgatgtatt gacgttggtc gaagcgctct acacggaaat gatcccagcg
     3001 gtcgcgccac acaaaaagct gctgtcttca ccctggccaa aattctcata tcgcgaggtt
     3061 gtggagcggt ttgggacgga caagccagat ttacgctttg gcatggagtt gatcgatgtc
     3121 agcgacgtct ttgcgaaaag cgaattcaag gtttttcagt cggctctgga agctggcggt
     3181 gtcgtcaagt gcatcgtcgc tccgaagtct gccaatatgt cgcgcaagga agtggacgca
     3241 ttgaccgagt ccgcgaaggc gcttggggcg aaaggacttg ccactttagc ggtaaccagc
     3301 gaaggcttga aaggaactgc tgcaaagttc gtgaaacctg aggaagtaga tgcggtcaaa
     3361 tcccgaacga atgctcagga aggagatctc atcctatttg cagcggacca gcgcgtagtc
     3421 gtcaataaag tccttggtgg gttgcgcctt atcttccgcg atgaactgga tcttgcagat
     3481 ccaactatta tggcatttgc ctgggtcgta gacttcccaa tgtttgcgtg gaacgaggag
     3541 gaaaagaaat gggatgcaga acatcacccg tttacaatgc ctcagttgga cgacctggcc
     3601 aagttcgaga ccaacccggc agaaattctt tcggatgcct atgacatggt ttgtaatgga
     3661 tatgaaaccg cgtctggttc gatccgtatc catcgcagcg acattcaact cagggtcttt
     3721 cagttattgg gtttgagcgg tgacgagatt gaaaataagt ttggtcacat gcttgaagcg
     3781 tttgaatttg gcgcaccgcc gcatggaggc atggcacctg gaattgaccg cctgatcatg
     3841 ttgcttgcgg acgaaccgaa tattcgtgaa gtgatcgcct tccccaagaa tcaaaccggg
     3901 cgcgacgtga tggcagatgc gccgtcagag gttgagccaa gacagttgaa agatttgcat
     3961 atcaaaattg cgtaacgata aacagaaagt taaacgatga acttgaaaga gttaattatc
     4021 aataaaaccg caaagtttgc atactgcacc gatggagcgt tgtggtatga agtggatggt
     4081 tttcgttttc cggttccttt tgaagataca gttggtgcat acttcgtgcc tgaacacaag
     4141 gcgatcaacc tcatgcgctg gatcagaaag cagcttgaag agtatgaggc tcaaaaaagc
     4201 atacggcagg caaattaggt aaaacgcgga tgaacgtcaa gccaggtgat gtgttttgga
     4261 tttcgcctaa ggaatcgaat gggattgctt ctgatcacac gcatccatat gtggttattc
     4321 atgatggtcc cataaacacc gtggtggttt gtgcattaac gtcgaatctt aaacgcgcca
     4381 aagagcccgg caatgttctg ctcgaagaag gtgaagcgaa tttgcccaag caaagtgttg
     4441 tggtggtttc gaaggaattt accctcgata caataatgct gggccaatac attgggacag
     4501 tgaatgaaca aaggataaat caagtgctgg ctggacttcg atttttgaaa tctatgataa
     4561 agcatcacgc gtaaggaaga acagcacagg cgctgcggcc aacggagcaa agtatgagca
     4621 aaaaacaaga agttcaagtt gatgaaaaac tggtccggga gatcgcctca ctggcacggt
     4681 tggatctcac agcggaagaa actgccacat ttgcaaccca attcaaagac attctggagt
     4741 acgtcaatat cctggatgag gtggagacag aatcggttcg ccctgcctat atgtcgtcgg
     4801 cgaataaaag tgtgacgcgt gaagatgaaa ttgaagagtc tgtgccgact gaggaattcc
     4861 ttgctaacgc tccactatca aaggatgatt atgtccttat tccacgggta cacatcgaac
     4921 aataacaatg gacttacatg agctaagcat acagcaagcc agcgaacttc tttcgagtcg
     4981 caaaatttct tcggaagaat tgacgcgcgc ctatctggaa cgcatccaca ggcttgattc
     5041 acaagtcaaa tcctacgtca cagtctcaga agaactcgcg ttggaacaag ccagagaagc
     5101 cgacaagcgc atcaagatcg gcaaaggctt gactccgctt acgggcattc cttattctgc
     5161 aaaagattcc atctcgaccc gtggggtcaa tacgacctgt tcatccaaga tccttgaaaa
     5221 ttacaagccg ttttacgact cgacggccat cagcaaactg aatgccacgt attctgtttt
     5281 gctgggtaag aacaatatgg acgaattcgg catggggtca tccacagaaa actctggctt
     5341 tttcaccacg cgcaatccgt ggaattatga ctatgtccct ggcggctcga gcggcggatc
     5401 tgcagcagcc gtagcggcgg gcctgactcc gttctcgctt ggggaagata ctggtggatc
     5461 tgtccggatg cccgcgtcat tttgtggaat taccggactt aagaccacgt acggacgtgt
     5521 ttcacgctat ggtttgattc cgcttgcctc ctcgtttgat acgattggtc cgatggcgcg
     5581 gagcgtatac gatgtggctc tggttctcga aacaatcgga ggtcacgacc cgaaagatag
     5641 tacatcgcgt attgaacccg ttcagcgcta ctcggagacg ctcgacaaag cggacaattt
     5701 gcgcgggcta cggattggaa ttccgaaaga gtattttgtt gaaggcttgg attcggcagt
     5761 tgatttttct gtgcgcgagg ccatcaggca aattcaggga atgggtgcag aggtcgttga
     5821 gatatctttg ccgcatacgc gctatgcgat cccagtctat tatttaatcc tgttcgcgga
     5881 agccagttcg aatttagcga aatatgacgg tgtacgtttc ggattatccg agagcaacgt
     5941 ggacaatttg ctggatgttt atttgaagac tcgcgaagct ggattcgggg cggagacaaa
     6001 acgccgcatc atgcttggca cattcgcgtt atccgccgga tattatgatg cctactattt
     6061 gaaggcacaa aaagtacgca cgctcatccg tcaggatttt cagaaagcct ttgaaacctg
     6121 tgacgcgctg atcacacccg tctcaccgac cacagcgttt cgtattggcg aaaaagtcac
     6181 caaccctctg gatatgtatt tatcggatgt atttgttgtt gccgtgaacc tggctggcat
     6241 cccggcactc tctgttccct gcggcatctc aaatggactg ccgatcggga tgcagattat
     6301 gggtccgcat ttgtctgaag agactctgct gcacatcggt cacatgtatc aggcacaaac
     6361 agattggcat caaaagcatc caccctgttg gaatgaataa tgacttttcg tcctgagcaa
     6421 agggtcgagt agagcgaata ttttctcgct cgtatcgaga cccgaagacg aagggcgggc
     6481 ttccaaaaga atgaaatata tcttggttat cagcgcttcg tctacatgcc tcggcttaca
     6541 ctcgacattc cgctcagcgc gaggaaaatt cttaatatga aatacgaacc tatcatcgga
     6601 ctcgaagtcc acgcggaatt gctcacacag tcgaagatgt tttgtggctg tgaagtcgtt
     6661 gattcgatta cagcaaagcc aaaccgttat atttgtcctg tttgtactgg tcaacccggc
     6721 gcactgcctg ttctcaacaa aaaggccatt gaactggccg ttcgagttgg gcttgcgctt
     6781 aattgtgaaa tccacacgga aagtattttt gcgcgcaaga attatttcta tccggatctt
     6841 ccaaagggat atcagatctc gcaatacgac cagccacttg ccacaaatgg gaaacttgaa
     6901 attgaaactg aagccggcat aaaggagatt cggattcgcc gggtccatct ggaggaggac
     6961 acagggaagt tatcgcacgc ggacgggcac tcgttcattg actacaaccg cagcggggtc
     7021 ccgttattgg aaatcgtcac cgagcccgat cttcgctctc ctgacgaagc caaagcttac
     7081 gcaacaaagc tgcatgcgat attgcgctat ctggatgtaa actccggcga tatggagaaa
     7141 ggtgtgattc gctttgagtc caacgtctcg gtgcgcgaag aaggcagcga ccaattcaat
     7201 acaaggaccg aaattaaaaa tctgaattcc tttcgcgcgc tgacgcaagg gaccgaatat
     7261 gaaatcaagc gccaaaccga aatttattca cgcggcggaa ccgtggaaca ggaaacgctc
     7321 ggatttaatg agtccacagg aaagacttac tcgcagcggg gcaaggaaag cgctcacgat
     7381 tatcgctatt tccctgaacc tgacctgccg ccacttgtgc ttgactctaa ttggattgag
     7441 tccatccgtg ctagtttgcc ggagttgcat gaagcaaaga tacttcgatt catcgaacgg
     7501 tatgaattga aagcacagga tgcacgacta ttaacgtctg aaagagctct cgcagattat
     7561 tttgagtctg tcgtagccaa atccaagagt tcgcctaagt ctgtcagttc atggatcgct
     7621 ggcgaattga tgcggcacct aaatgactct ggaattggga ttgaaaatat cacgctttct
     7681 cccgaatcat tcgccaggct aattgatatg acgactgaca aagtcattag cggcaactca
     7741 gccaaagttg tgtttaatga aatgctaaaa aacggcggcg acccggagca aatcgtcaaa
     7801 gacaagggtc ttgcacaagt ctcggatgaa ggttttgttc aagaagctgt gatgaaagtg
     7861 ctaaatgaca acccctccga agtggagaaa tatctcgcag gtaaagacac cctactccaa
     7921 tggtttatgg gacaagttgc gcgcgcaaca aaaggcaaag ccgatccaat actgacaaaa
     7981 aagatactgc ttaaagcgct ggagagccag aaaacataaa gaagtaattg tcgctttctg
     8041 tggtacgtca attactcatc tgacaaatac agggactcgc catcgtccat tcccccaata
     8101 atcacaccac cattcactga ggccccactg acctcattcg gaataactat gataggttca
     8161 atttttgatt ttaatatcga tgtgtcttca tacagatatg caaatactgc tggaaataga
     8221 tcctgtaaaa ctgtaatatc gtcatacagc acaaaatcat ctgttggcca cctgattgcg
     8281 aggaaagttc catatcgatc ttgaatatcc aaccgggaaa tttctgaaat atcgtaaacc
     8341 cctgtcgtta catggcaatt ttttgtcaaa tacgggccat gatccccagc cacaatcaca
     8401 attgcgccag gatcattctc aataatcaaa ttgatatctt gtttcatctc caaattagcg
     8461 cgcctcaatc tttcttcaaa caggtcaacc tcattgaaca ggcaagctcc tgaattctga
     8521 ctatgagatg gcagatcagt atgcatataa acaaaaaatt gatttctagg tctgtctgcg
     8581 aaaatactct gcttcgtgtc aagaaattcc gctctaggta ctttattaaa cccgattgtc
     8641 tcaatgttaa atctgaactc gccgatcaaa atggccttca acaactgtat ataaggtgga
     8701 atactccgct ccgggcttga ataatcatag ctttcaccgt aacccaagaa catgtaatcg
     8761 ctagaaaata agccgtaagt tttataacca gcctctttca atattgtgtg cgttattcca
     8821 tctcctgata cggctcttct tagatcccca taatactccg ttgaggcatt gagaaccctg
     8881 ctcatagtct caacgctcga tgcgtttata gagtaagtat gaggataaag ttcgaagcct
     8941 tgttgactta aatagctttc ttgcgcgccg ttatcaatac cataggcaag cattgtttca
     9001 ttgggaacat atgaatcata gataagtaaa tatatatttg gcgtcattgc gggcattctt
     9061 tcagctacta tcgatagaag cacgttttcc tccctaggaa cgggtgagta atccacgcgg
     9121 gcatcctgag acaataacgg agcaacgcca ctggctatga aattaactac aataaatgag
     9181 tacaaaactc ttctttggtt gcttttgtat aaaaaccaac tgatcacgaa tacgaaacca
     9241 aaaaccgtga cttgaatcgt taacgacccc atttcaaacc aggcgaaata atcgctgatg
     9301 gaagccatac tggtaatcgt aaacacaaaa gccagaccca gcaccatcag tgtccgggtt
     9361 gatatcacac tactgagaac agcagggata gcgaagatat aaaagctcga aaaaaacaga
     9421 aaaaatataa gtacatatac ggagtctaca acagacaaaa gatcttgatt gtgtaaaaca
     9481 tattgaacga ccggtgttaa aggaagcaga agcagcaaaa aatcttcacg gttaattttt
     9541 tcaagagaag gtttgaaggc gagcttgccg cccgttctta tcttgagcat tacaagaaaa
     9601 atcaaggcaa ccgccaccaa gagaaacaac ccatagattg ccaagctgct tgcaaagaag
     9661 taacttacac ccttaagcag aaagcgggaa gaaaaatatg caaatgaacc gctataacat
     9721 acgagcacaa tcacaggcac aagaatattt gagaaataga aattggcatc aattttcttt
     9781 ttcaattgag tgatcatatt ttgaacataa aatccttcga aggaaaattg gattcgatct
     9841 taatcgtgga gttgggcaca aagtcagttt gctgcttttt tcgagcgtaa aattgcttag
     9901 gcgtagaata ttgggagtcc tgctatttta gcagaagaaa aagctgagct agtatttcaa
     9961 gcaaaggatg gagccgtaag aacaaaaaga gacttttagg agtctctttt ttgattcttc
    10021 atctttcttt tctatctttt ctatccttcc tctttttgcc atcatcgtcg tgtgccatca
    10081 tatcaattgc aatcgcgacc gacaggatga gaatatcgtt ctgtcctgga gcaatctcca
    10141 cgccgtaggt atccgtaaag gtaaaccact gtttggacac ctgggcaacc tggtttcgcc
    10201 cctgcatgat tgcgtattca aagtcaaaga tgttgccgcg cacatccagg tctggtccgt
    10261 ctttgacgtt gaccgaccag cggtcacgca atggagcaat gagtgccttc ctgaccgtgg
    10321 caatttgatc cccatctgca tcctggattg ccattgtatc tttgatcgct aacaacctct
    10381 cctgaatttg agccagcttc cttccgttca gatcttcaaa gaccagcgtc ttgcggattc
    10441 gcagtacctt accatccacc ttgaagactc gcttgccctc agcattttca atccagaaat
    10501 catctccgat ggagatcagg ttttgacgta tcttgtaacg gttgctcata atttctcctt
    10561 ttgaagccag taggctccat atgtttatct acgctattgt cgtgttgaag ttgtgactga
    10621 tatctgccgg gacttatcag aatttacaaa atgttgaccg acgatttgaa tggaatagtc
    10681 aggaactggc gatagcagcg atttcagttt taatactcct tcattgcaaa acctggtcgg
    10741 agggttaatt tatgctagga tagccgatag ggctttgacc atggacgaac agactttcaa
    10801 taacctgcgt ctacgattag gtgatgaaca cctggggaag cggatgcgtg cccaggtgga
    10861 tcacattatc aatgtgacgg gtcagggatt gggatctttc cattacgaaa acgtgccttt
    10921 gtttgtccgg gttgtggatc tcgcgcttcg agtcagtggg ttaaaaaagt acggtcagaa
    10981 acaagccttg aaatttgtgg tgcgggaaaa tcaggtacct ttccctgacc tgccaaagtc
//
LOCUS       DASTJP010000266         5766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1217713, whole genome shotgun sequence.
ACCESSION   DASTJP010000266 DASTJP010000000
VERSION     DASTJP010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217713"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VFQ23_08060"
     CDS             complement(<1..778)
                     /locus_tag="VFQ23_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906580.1"
                     /translation="MMRPQKSFILVLICILSACAQNPVIESAQSATPHIAKTQAIEAT
                     LTPLPTFSFSNTPLPTFTPFPLPATLVVQPTLDSTPQVPSEWTFDKINPIFYGKSLLG
                     ATFGGNWLYPREVYSLLGAPQVYDFYDQNFQPFQATVVVSEPPPGGAHQCDWLGIKFV
                     DEGIGDISPVLGVKPGQSVIFRPVQDIAQDSGPYEQYLQEWLLLQGIQSPELVIENIL
                     RVDLEGDGVDEVILVATNIEDSMGGLHVAGAGKYSVVLMRM"
     gene            complement(968..4024)
                     /gene="glyS"
                     /locus_tag="VFQ23_08065"
     CDS             complement(968..4024)
                     /gene="glyS"
                     /locus_tag="VFQ23_08065"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET9906581.1"
                     /translation="MPLNFQQVILTLQQFWAARGAVLWQPYYTQVGAGTMNPATFLRV
                     LGPEPWNVAYVEPSVRPDDGRYGENPNRFQVHYQYQVILKPDPGNPQEIYLDSLKAIG
                     IDPRQHDIRFVEDNWEQPAISAWGLGWEVWLDGQEITQFTYFQQVGGIALDPVAVEIT
                     YGLERILIGLHNVKAAWDLPWNDSITYGEVRRQEEFEHSKYYYELGSVDRMKEMFEHY
                     EHEAEAALTHEPPLVLPAHDYVLKSSHTFNILDARGVIGVTERQAYFRRMRELARRVG
                     EAYLAQREHLEYPLLRDTETGKSGIGTRESGTAQLPTPNARLPLILEIGTEELPPGDV
                     DTALTQLRERVPSLLKELSLEHGSVNIHGTPRRLAVSIADLSPNQPDREDLVKGPPAD
                     KAFDKDGVALPAGMGFAKKNNVNTKDLQVREEGGGKYVFASVKQTGRPTPEVLAEALP
                     KLIDSIKFEKSMKWLPPSRRGGNDSGVAFSRPIRWIVALLGDQVIPFEYAGVRSSNIT
                     RGLRPYDSPEMVIASADKYFDVIRAAGITIDFEERNASIVEQVNQAASLVGGEAIIEE
                     GLLSEVTNLVEMPTAVMGGFDKEYLALPRDVLISVMKKHQRYFPVTLTPTPIPKGEGQ
                     GVRGEGSLLPHFIAIRNGDDIGIDVVRQGNEHVLGARFADANFFVREDVKLKLEEYRP
                     KLSTLIFHAKLGSMLDKSERIEKLVNQLIPMLGLENDEAIFARRAAHLAKADLVTQMV
                     TEMTSLQGIIGREYALRSGEQADVANAIGEQYKTVPPSKIGLAIALADRLDSLVGLFA
                     VGLAPTGAKDPFGLRRAAIGVVQPLIEQDIDFDVVEAIKKSAKTQPIKVGEEIQNQIL
                     NFIAGRLRVVLNDMGYKHDVVDAVLAEQSANPAKSAEAVKQLQAWVEREDWSTILPGY
                     ARCVRIIRSAKLDNRRLTIDDKKFAEQAERDLYKAIQSSVVHRPSSVNEFLNIVVQLI
                     PSINAFFDKVLVMAEDEKVRQNRLALVGQVANLSDGLADLSKLEGF"
     gene            4122..4244
                     /locus_tag="VFQ23_08070"
     CDS             4122..4244
                     /locus_tag="VFQ23_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906582.1"
                     /translation="MTQNQRPRQKSCFSTDEASRAHIEGRTWDEMPVNVGLVKA"
     gene            complement(4296..4628)
                     /locus_tag="VFQ23_08075"
     CDS             complement(4296..4628)
                     /locus_tag="VFQ23_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HET9906583.1"
                     /translation="MSPRDWRDRIRDILEAIAEIQKFTLGMDYKTFKDDDKSIRAVEM
                     NFIIIGEAANQIPEDIEEKYTAIPWSLMRAMRNRIVRVYFNVDEKLMWDTIQNDLPPL
                     IPELEKLL"
     gene            complement(4618..4911)
                     /locus_tag="VFQ23_08080"
     CDS             complement(4618..4911)
                     /locus_tag="VFQ23_08080"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9906584.1"
                     /translation="MKQDAVLQVLKQKNAEITSKFGVKSLLLFGSVARNEATSSSDVD
                     LLVEFSRPVGYFGLFALQDYLEKLLGCQVDLGTPDSLKPYIKDRVMGELIRVT"
     gene            complement(4944..>5766)
                     /locus_tag="VFQ23_08085"
     CDS             complement(4944..>5766)
                     /locus_tag="VFQ23_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906585.1"
                     /translation="AIIAAIIGVLGTIVVTLIQFYATRQTTLPTPTSSLTAFTATSTN
                     TVVNTNTVSPNTPTFTPVPATDTPIPTPTGIPPVSLGTDFPDGCISTSWKPYPAEIAV
                     TERGDGCWKEPVYVFYAENGDLDVLAQGNNEEVQIYGLFAPLPESGRVNVTVRLNELT
                     NVDVWLGIFSEQDINSQGLLMTVPTGNVLKRVIVQKDPRTNETITSTSLLEQGRGFSF
                     SFTFNANSARGILNPSVFFTESVSIPSSQKWLFLGYKSLPGYYRIEGRFLEFELE"
BASE COUNT         1410 a         1478 c         1438 g         1440 t
ORIGIN      
        1 ccattcgcat gagcaccact gaatatttcc ctgcacccgc gacgtgcaga cctcccattg
       61 agtcttctat attggttgcg acgaggatca cctcatccac gccgtcgcct tccagatcta
      121 cccgcaagat gttctcgata accaactccg gggactgaat gccttgcagt aaaagccact
      181 cctgtaaata ttgctcgtac ggtccgctat cctgcgctat atcttggacc ggtcgaaata
      241 tcacagattg cccgggcttt acccccagaa caggtgatat atcaccgatc ccctcgtcta
      301 caaattttat gcccaaccaa tcacactgat gcgcccctcc gggcggtggt tcagacacca
      361 caactgttgc ttgaaaaggc tgaaagttct gatcgtaaaa atcataaacc tgcggggcgc
      421 caagcaggga atacacttcg cgtggataca accagttacc gccaaacgtt gcacccagaa
      481 gggattttcc ataaaagatg gggttgatct tgtcaaaggt ccattcagaa ggaacttgcg
      541 gtgtgctatc aagggttggt tgaacaacca gcgtggcagg aagcgggaac ggagtgaacg
      601 ttggtagagg cgtatttgag aaagagaatg tgggcagtgg agttaaagtt gcctcgatgg
      661 cctgggtctt tgctatgtgc ggcgtggctg attgcgcaga ttcaatcaca ggattttgcg
      721 cgcaggcgga aaggatacaa atcaaaacga gaataaatga tttttgcggg cgcatcatga
      781 tttcctccac agctggtgat aacctttgcc gcttgcttcc taattacacc agcttttcaa
      841 actgctttgt aagccattat aaaaaattta aagacacttg attacgcggc actatgtaga
      901 acggtatccc tcctgggatt gttccagaaa aaaagcccaa ccttttgagg ttgggcgtat
      961 tgaccatcta aaatccttcg agtttactta agtccgctaa tccgtcagaa agattagcaa
     1021 cttgccccac cagcgccagc cggttctgcc tgaccttttc atcctctgcc atcaccagca
     1081 ctttgtcaaa gaaggcattg atggatggaa taagttggac cacgatgttc aggaactcat
     1141 ttacggacga tggacgatgg acaacagacg attgaatagc cttatataaa tcacgttcgg
     1201 cctgttcagc aaacttctta tcgtcaatgg tcaatcgtcg attgtctaac tttgcggagc
     1261 ggataatgcg gacgcaacgc gcataacccg gaaggattgt ggaccaatcc tcacgctcga
     1321 cccaggcttg aagctgtttc accgcttcgg cagatttcgc agggttggct gattgctccg
     1381 caaggactgc atcgacaacg tcatgcttgt atcccatgtc atttagcacc actcgtaatc
     1441 gcccggcgat aaaattcaga atctgatttt gaatctcttc acccactttt atgggctgtg
     1501 ttttggcaga cttctttatc gcctccacca catcgaaatc aatgtcctgc tcaatcagtg
     1561 gctgtaccac accgatcgca gcgcgccgca acccaaacgg gtccttcgca ccggtgggtg
     1621 ccagccccac tgcaaagaga ccgacgagcg aatcgagtcg atcggccagc gcaatcgcca
     1681 gaccaatttt cgacggtgga acggttttgt attgctcacc gatcgcattt gcgacatctg
     1741 cctgttcgcc acttcgcaat gcatattcac gaccgatgat tccctgcaga gatgtcatct
     1801 cggtgaccat ctgtgtgacg agatcagctt tggctaaatg ggcggcgcgg cgtgcaaaga
     1861 tggcttcgtc gttttcgaga cccagcatgg gaatcaattg attgaccagt ttctcgatgc
     1921 gctcagattt atccaacatc gagccaagtt tggcatggaa gatcaaggtg gaaagtttcg
     1981 gtcgatattc ttcgagcttg agtttgacat cttcacggac gaagaaattt gcgtcggcga
     2041 aacgcgcacc aaggacatgc tcattgccct ggcggaccac gtcaatacca atatcatcac
     2101 cgtttcgtat ggcgatgaaa tgaggcagca agctgccctc gcccctcacc ccttgcccct
     2161 ctcccttagg gataggggtt ggggtgaggg taacagggaa atagcgttgg tgctttttca
     2221 tcaccgaaat cagcacatcc ctcggcaagg ctaaatattc tttatcaaac ccacccatca
     2281 ctgccgttgg catttcgacg aggttcgtca cttcgctgag caatccctcc tcgataattg
     2341 cttcaccgcc aaccagtgac gcggcttgat tcacttgctc tacaatcgaa gcgttgcgtt
     2401 cctcaaaatc aattgtgatg cccgcagcgc gaatcacatc gaagtattta tccgccgatg
     2461 cgataaccat ctctggtgag tcatacggac gaagtccgcg tgtgatgttg gagctccgca
     2521 cgccggcata ttcaaatgga atcacttgat caccaagcaa agcgacgatc caccgaatcg
     2581 ggcgcgagaa cgcgacacct gagtcatttc ccccgcggcg agaggggggc aaccacttca
     2641 tggacttctc aaacttgatg ctgtcgatta atttcggtaa agcttctgca agcacttctg
     2701 gcgtggggcg tcctgtttgc ttgacgctgg caaagacata cttgcctccg ccttcttcgc
     2761 gaacctgaag gtctttcgtg tttacattat tcttctttgc aaagcccatc cccgcaggta
     2821 gggcgactcc atctttgtcg aaggctttat ccgcaggcgg ccctttgacc aggtcttcgc
     2881 ggtccggttg attcgggctg aggtcggcga ttgaaactgc gagccgccgc ggcgtgccgt
     2941 gaatattgac ggaaccatgc tcgagcgaaa gttccttcaa cagcgacgga acccgctcgc
     3001 ggagttgagt cagagctgtg tccacatccc caggcggaag ctcttcggta ccgatttcca
     3061 gaatcagcgg gagtcgtgca ttgggagtcg ggagttgtgc cgtacccgat tcccgagttc
     3121 caattcccga tttgccggtt tctgtatccc taagcaacgg atattccaaa tgttcgcgct
     3181 gtgcgaggta ggcttcaccc acgcgcctgg caagctcacg catgcgtctg aagtatgcct
     3241 gtcgttcggt cacaccgatg acgccacgcg catccaggat gttgaaggtg tgtgagcttt
     3301 tgagaacgta atcatgcgca gggaggacga gcggcggctc gtgcgtgagg gcagcttcgg
     3361 cttcatgttc gtagtgctcg aacatttcct tcatgcgatc tacactgccc agttcataat
     3421 aatacttgct gtgctcgaac tcctcctgcc ggcggacttc accataggtg atcgagtcat
     3481 tccacggcaa atcccacgcg gctttgacgt tgtgcaaacc gatcaggatg cgttccagcc
     3541 cgtaggtgat ttcgactgcc acagggtcga gcgcgatgcc gcccacctgc tgaaagtacg
     3601 tgaactgtgt gatctcctgc ccatccagcc agacctccca gccgagcccc caggcagaga
     3661 tggcaggctg ttcccagtta tcctccacaa agcggatgtc atgctggcgc gggtcgatgc
     3721 cgatggcttt gagcgaatcg agatagatct cctgcgggtt gccagggtca ggcttgagga
     3781 tgacctggta ttgataatgt acctgaaagc gattggggtt ctcgccataa cgcccgtcat
     3841 cgggccgcac ggatggttcc acgtacgcca cattccacgg ctcaggacca agcacgcgta
     3901 aaaaggtggc agggttcatg gtgcccgcgc cgacctgggt gtagtagggc tgccagagca
     3961 cggcaccgcg cgctgcccag aattgttgga gggtgaggat tacttgttgg aagttgagag
     4021 gcatggtgga tttatgattt cagattttgg atttatgatt gccagaggat tataacttat
     4081 ggggaataga aaatggatca tggacaatag tcgcaagatc gatgacacaa aatcagcgtc
     4141 cgaggcaaaa gtcctgtttt tctacagacg aagcgagcag ggcacacatc gaaggtcgca
     4201 cgtgggatga gatgcctgtc aatgtggggt tggtgaaggc gtaaaacctt taaacacgaa
     4261 ggacaccaag aggcacaaag gcttttgaat aagttttaca gtagtttttc cagttcggga
     4321 atcaaaggcg gtaagtcgtt ttggattgta tcccacatca atttttcatc cacattgaaa
     4381 taaacacgca caatgcgatt ccgcatggcg cgcataagac tccatgggat tgccgtatat
     4441 ttttcttcaa tatcttcagg aatttgattt gcagcttccc caataatgat gaaattcatt
     4501 tcgacagcac ggattgattt atcatcatct ttaaaggtct tgtaatccat gccaagggta
     4561 aatttctgaa tctcggcaat tgcttcaaga atatctcgaa tccggtcacg ccagtctcta
     4621 ggtgacacga atcaattccc ccattacgcg gtcttttata taaggtttca aactatcagg
     4681 ggtgcctaag tcaacttggc agccaagcag tttctcaagg taatcttgta aagcaaataa
     4741 gccgaaatag ccaacaggac gactgaactc gaccagcaag tccacatcac tgcttgatgt
     4801 ggcttcattc cttgccaccg agccaaaaag caacagagac ttcacaccga atttacttgt
     4861 tatttcggcg tttttttgtt tcaagacctg gagaactgca tcctgcttca tatcaactat
     4921 tataacgctc aatccaaacg gccttactcc agctcaaact caaggaacct gccttcgatg
     4981 cggtagtagc caggcaagct tttgtacccc aggaagagcc atttttgtga ggagggaatg
     5041 gagaccgact ctgtaaaaaa cacacttggg tttaagatgc cgcgcgctga gttggcgttg
     5101 aaggtaaagg aaaatgagaa tccacgtccc tgctcaagca agcttgtact agtaatcgtt
     5161 tcattcgtcc gaggatcttt ttgaacaata acacgtttca gcacgttacc ggttggaacg
     5221 gtcatcagca atccctgtga attgatatcc tgttcagaaa atatccccag ccagacgtcc
     5281 acattcgtca attcattcaa ccggaccgtt acattcactc tcccgctttc tggcagggga
     5341 gcaaatagac cgtagatttg gacctcttcg ttgttgcctt gtgccagtac atccagatct
     5401 ccattctccg cgtagaaaac atacaccggt tctttccaac agccatcgcc acgttcagtc
     5461 actgctattt cggcaggata gggcttccat gatgtgctga tacagccatc cggaaaatct
     5521 gtcccaagtg aaacaggcgg aatccctgta ggagtgggaa ttggagtatc cgtggctggg
     5581 acaggtgtga aagtgggtgt gttgggtgac acagtatttg tgttcacaac tgtgtttgtg
     5641 gaagtcgctg taaaggccgt tagcgatgaa gttggtgttg gaagtgtagt ctgacgggtt
     5701 gcatagaact ggatcaatgt tacgacaatc gtgcctagta caccgataat agctgcgatg
     5761 atcgcc
//
LOCUS       DASTJP010000267         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_654608, whole genome shotgun sequence.
ACCESSION   DASTJP010000267 DASTJP010000000
VERSION     DASTJP010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654608"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFQ23_08090"
     CDS             <1..519
                     /locus_tag="VFQ23_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9906586.1"
                     /translation="TCQRGDFIGERDRSLFLFLLDTGARAREACNMNIKDADLNTGAV
                     MIRYGKGGKTRMVFIGRTTRRAIRAYLRLRHDSSPALFVSKDEERLTYDGLRLLLERR
                     AIRAKLSNKPKLHGFRRAFALNMLRSGVDIFVLQRLMGHSDLQVLRRYLAQNDEDNQL
                     AHMRGSPVDNNF"
     gene            complement(1417..1728)
                     /locus_tag="VFQ23_08095"
     CDS             complement(1417..1728)
                     /locus_tag="VFQ23_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906587.1"
                     /translation="MKHELIEELRQRNSRIIEAIIKKEHAVCPGSIALIGIAGSFCHG
                     DIHERSDLDLCIVINDNDGWKIADCFILNDVGFDVCAGILMTFFTLSAAFSPTVRNGI
                     E"
     gene            complement(1796..2860)
                     /locus_tag="VFQ23_08100"
     CDS             complement(1796..2860)
                     /locus_tag="VFQ23_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9906588.1"
                     /translation="MIEPSVHPRYSALIHLFVAATILTLLFSAPRETARAQTPQTETP
                     QAIQPPTPAEIIDAINALRLSYGLPALAVHPVLMALAQRQADGIAAGLHGHWRPYGLT
                     LGQLLIMEGYPLAGDLTQDGYRSENWIVCMTAQGAIDAWLSDDEHSNTMLSEYRSDIG
                     AGVSIMRDEWGQDQIYVVIETALQTRSGQQQSLARDFLTRLPSIINGTTSINGTPFPL
                     SEGQYMIPVIRATARPDGDVVHEVQYGQSLWSIAITYGTTIEQIQKLNNLGDSNIVYE
                     QQLLLVQKGATQPAVIPTQTATAIISSTPSPTVTQTETATGTAAAPMGKSSTIVVFLI
                     IGVSVLIAGVGATLRMKPTP"
     gene            complement(2915..>3280)
                     /locus_tag="VFQ23_08105"
     CDS             complement(2915..>3280)
                     /locus_tag="VFQ23_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906589.1"
                     /translation="GAALHVMDIQSGEVSLLFDINYNGTVVVSPDGKQLAYEEMLPLD
                     KYGLFVSDLDGSNRKLLADGDPYTVTVPSWSPDGNWVIATVHDPDVNKQPALALIQVD
                     TCQIIPLPNLSGYVTSWLP"
BASE COUNT          837 a          810 c          810 g          823 t
ORIGIN      
        1 acctgtcaac gaggggattt cataggcgag cgagatcggt ctcttttcct gttcttgctt
       61 gacactggcg ccagagcacg agaggcctgc aatatgaata tcaaggacgc agacttgaat
      121 accggagcag tgatgatacg ctatggaaaa ggcggcaaaa caagaatggt ttttatcgga
      181 cgcacaacaa gaagagctat acgcgcttat ctgcgcctgc gtcacgactc ctctcctgcc
      241 ttgttcgtct cgaaggacga agaacgtctt acctatgatg gcttaagact gcttctggaa
      301 cgaagggcta tacgagccaa gctaagtaat aaaccaaaac tacacggttt ccgtcgtgcc
      361 tttgctctta acatgctgcg cagcggtgtg gatatctttg ttttgcaaag gctcatggga
      421 cattctgatc tgcaggtgtt gcgccgttac cttgctcaaa atgatgagga taatcagctg
      481 gcgcatatgc gaggaagtcc ggtcgacaac aatttctgaa ttccagcttt gtgtagagtt
      541 ctattgtgaa ttacagttgg gcctgtccag agattatcat aattggatgc ctccccctca
      601 gagctttgtg tttgatcgta ggttcgaagg tttcaagcac atgaatttta attccacacg
      661 gctagctcaa aaggctagcc atgtggccct agtgggagtg ctgagatttg cgaagtccat
      721 cagtgggaac cgggccccat aaacaaaaga ctccctcggg ttgggagtct caatgtctgt
      781 cgggtgccag gattttaacc ccggacatca cggacccgaa gtatcttagc tgtttaggct
      841 gttaaggggt gcatggatac tcaaccagga aatccatacg ctcctgttgg agcaactata
      901 tggggatata ttatcactgt ttttcgcgga aaacaagcac aatacctgtc attccaaatc
      961 aaacaagatc ttcttcaaga cgtgaacaca ataactacga gaaaggtatg gtgctttcaa
     1021 cggaaataat tggcttagct attgtcgaag gcagcagctc cgggactcat tgatttagta
     1081 tcacatccaa aattggttgg agcattagta acaggggttt caacttaaac actgaattca
     1141 gggaggaacg gttagagccc aaaataatta cgtagccagg aaagccagca tcatgcgcac
     1201 acttaaaact aagaatcttt cccttgtctt cccatacaaa agccaaacat tgcgaggcgc
     1261 gcaacgcaca ttacagggcg tcaggttgcc actgctcctt ctggaagtca tgcacagagt
     1321 tgatcagtgt atgaatcgcg accacgtaat attctgttgc gattcgaacc atcgtgataa
     1381 ttctaagtag ctacaatatc aagcgcgcat tgtcgcttat tctatgccat tgcgaactgt
     1441 agggctgaac gcggcagaca aggtaaagaa agtcatcaaa atgccagcac aaacatcaaa
     1501 cccgacgtcg ttcaaaataa agcaatcagc gatcttccag ccatcgttat cattgatcac
     1561 gatacacagg tccaggtctg accgttcatg aatgtcacca tggcaaaatg acccagcgat
     1621 gccaatcaag gctatggagc ccgggcagac tgcgtgttcc tttttgatga tggcttcgat
     1681 gattctcgag tttctttgtc tgagctcttc aatcagttcg tgtttcattc gttgtccctt
     1741 gtcgatgatt catatggttc cacggcacgc tatgatgtcc aaactaggat ttacctcacg
     1801 gggttggttt cattctaaga gtggctccca cacccgctat caatactgag acaccgataa
     1861 tgaggaacac cacgattgtg ctgctcttcc ccatcggcgc ggcggccgtt ccggtcgcgg
     1921 tctccgtttg agtaacggtt ggactgggcg ttgaggagat aatcgccgtt gcagtttgtg
     1981 taggaatgac cgcaggctgt gtggctcctt tttgcaccaa cagcagttgc tgctcataca
     2041 cgatattact atcaccgaga ttgttcagct tttggatttg ctcgatcgtc gttccatacg
     2101 taatggcaat actccacagc gattgaccat attggacttc atgtactaca tcgccatcag
     2161 gccgggccgt ggctcggata acagggatca tatactggcc ttctgaaagc gggaacggtg
     2221 ttccattaat ggaagtggtt ccattgatga tactggggag ccgtgtaaga aagtcgcgcg
     2281 ccaaagattg ctgctgcccg ctgcgagtct gcagcgccgt ttcgataacc acgtaaatct
     2341 gatcctgtcc ccattcatct ctcatgatcg atacccccgc cccgatatca ctgcgatatt
     2401 ctgagagcat ggtatttgaa tgttcatcat cggacagcca ggcatcgatc gcaccctggg
     2461 cagtcatgca aacgatccaa ttctcagagc gataaccatc ctgagtcaaa tcccccgcaa
     2521 gaggatatcc ctccataatg agcagttgcc caagcgtcaa gccatacgga cgccaatgtc
     2581 cgtgcaagcc agccgcgata ccgtctgcct gtctctgcgc gagcgccatg aggaccgggt
     2641 gaactgccag cgctgggagt ccataagata gcctcaaggc attgatcgcg tcgatgattt
     2701 cggcaggcgt tggcggttgg attgcttgtg gagtctcggt ctgcggcgtt tgggcgcgcg
     2761 cagtctctcg cggcgcggag aatagcagcg tcaatatcgt cgccgcaacg aaaagatgga
     2821 tcaaagcact gtaccttggg tggacgcttg gttcaatcat atgaacgtca tgatgggacc
     2881 tcagggtttc ccaaccctga ggtcccgatt ctttttaagg caaccaggag gtgacgtacc
     2941 cactcagatt cggcaatggg ataatttggc aggtatccac ttggatcaat gccagcgcag
     3001 gttgtttatt cacatccgga tcatgaacag tagcgatcac ccaattccca tctgggctcc
     3061 aggaagggac agtcacggta taagggtcgc cgtctgccaa cagtttgcga ttggagccat
     3121 ccaaatccga aacaaacagg ccatacttgt caagcggcag catttcttca taggctaact
     3181 gttttccatc tggagaaaca acgactgtgc cgttgtaatt gatatcaaac aacaggctga
     3241 cttcacccga ttgaatatcc attacgtgca aggcagcgcc
//
LOCUS       DASTJP010000268         4799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_38716, whole genome shotgun sequence.
ACCESSION   DASTJP010000268 DASTJP010000000
VERSION     DASTJP010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38716"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="VFQ23_08110"
     CDS             <1..1677
                     /locus_tag="VFQ23_08110"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET9906590.1"
                     /translation="KLNQSLSYPINGKILFRHGEQVWVATPAQLGMVFDPSSSAVTAY
                     ELGRRGGLFGALAGQIRAGGDGARIPPVILFDQRVAFQYLSQISTQINQPMMEASLKL
                     EGTNVVTQPGQVGRELKIDATLLYLGSQLQTFTDGEVQLVVQEIQPQILDVSGQADVA
                     RQVLSQPLTLAIPNATSGDPAPYVYNVEVLANLIGVQPVRNGDQVGVQVVLDPTRLRN
                     LLVPIKTNVDRLPLNAKFIFNDETRVLDLMEDSKVGRTMDVEASIQAINDALLRGEHT
                     VNLVINEAQPQVTGTTTGQQLGVTELIHSETSYFYGSSQERMQNIQAAAERFHGVLVA
                     PGETFSMGATMGDVSLDNGFAEALIIYGGRTIKGVGGGVCQVSTTLFRAAFNAGFPIV
                     ERTPHAYRVSYYEKIASGAVDPRLAGLDATVYFPLVDFKFTNDSPYWILMETYMGSGS
                     LTWKFYSTKDGRTVTWDTTGPVNVVSAPPPSFEQNPELSKNEMKQVDWSANGADVTVT
                     RTVWQNGAVLYQDTISTHYEPWQAICEYGPDSKDPEKLAREKNLCGNPSA"
     gene            1747..1965
                     /locus_tag="VFQ23_08115"
     CDS             1747..1965
                     /locus_tag="VFQ23_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906591.1"
                     /translation="MVSETLIEILRCPNCVRTTGGLLKLHKDTWFICQDCSRKYPIVD
                     DIPVMLIDEGDKWVSVAEGELPVPPPAK"
     gene            1967..2542
                     /locus_tag="VFQ23_08120"
     CDS             1967..2542
                     /locus_tag="VFQ23_08120"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9906592.1"
                     /translation="MKTVQDLLNDLTSGDETRAEDAVSDLIEKGEEAIPALLEMTKSG
                     DADKRWWALRILAQSPHSQTEWLIPFLNDPAADVRQCAALGLSLKPDESAIEPLVRAL
                     SDEDTLVSNLAVKALVNLGKAAVPSLIENVKGGSQSSRILALRALVEIRDHRAIPVMM
                     QVMSEESALLQYWAKEGLERLGLDMVYMKPV"
     gene            2539..3999
                     /locus_tag="VFQ23_08125"
     CDS             2539..3999
                     /locus_tag="VFQ23_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9906593.1"
                     /translation="MNKTNFFNNLKFSRPSNWQIVFWVVAVILVVGGFFFVRGLVTCW
                     IITPLPGSPPSTCGTVTSGNNGPVITTEDGTPAPSVEDLPPPIVEVPASDLPPAWDGA
                     SRITVLIIGLDARDLDTSAPRSDTMILLTIDPLSKTAGILSVPRDMWVNIPGFGYSRI
                     NTAYSSGEGNRLPGGGPELARKTVEQFIGVPIQYYAQVDFNTFIQFIDRIGGIDIHNT
                     EELRLDRIGSGNDKIKLTCCGIRHLPGEAALAYARHRKGGEGDFTRAQRQQKVIIAIR
                     DKVFDPANFPTLIPQADDFYNEFSAGIRTNMPFDVAIRLAVLAREIPVENIKRGAIDT
                     TMVAFDNVVLGGQDAAIMKPLPDKIRVLRDEIFTSGGAISPLAAGGDPLTLAKADTAR
                     IRLLNGSFSPGLETTTGNYFISQGLAVTEVGPADRAYDRTTIVLYSPKLYTLKYFQTL
                     FAIDSNALILIRPDPSSTVDVEVRLGNDWANSNPIP"
     gene            complement(4069..>4799)
                     /locus_tag="VFQ23_08130"
     CDS             complement(4069..>4799)
                     /locus_tag="VFQ23_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906594.1"
                     /translation="AAAYAATGDLTRAQARLALLGDNNPIEALNAQAQRMRASGTGGV
                     SFEEADQVVALAAALDQNGINPPRSTDTETAVSETVDSTSSPPTSTAALPSDEPPLEL
                     TETPASVETPLDTPVAATPRPTRTPVATFGAPFGLTGQDSVCDPNLPDGLLQVLILNS
                     NRRQMPGIEILITWEGGAENFFTGLKPELGNGYADYIMTPDITYTVQLRAGSDIASGL
                     TAPTCQTPSGEAFFGGIKLTFQQP"
BASE COUNT         1116 a         1183 c         1309 g         1191 t
ORIGIN      
        1 aagttgaacc agtcactatc ctacccgatc aatgggaaaa tcctctttcg acacggtgaa
       61 caggtgtggg tggcgactcc tgcgcaactg ggtatggtgt ttgatccatc ttccagtgcg
      121 gtaaccgcct atgaacttgg cagacgaggc ggattgttcg gcgcgttggc aggtcagatc
      181 cgcgctgggg gtgacggtgc caggattccg ccggttattc ttttcgatca acgtgttgct
      241 ttccaatatt tgagtcaaat atccacacag atcaatcaac ctatgatgga agccagtttg
      301 aaactggaag ggacgaatgt ggtcacacag cccggacagg taggtcggga actaaagatc
      361 gatgccacct tgctttacct gggctcacaa ttacagacct ttaccgatgg tgaggttcag
      421 ttagtagttc aggagatcca gccgcagatt ctggatgtga gcggacaggc cgatgtcgcg
      481 cgtcaggttc tcagtcagcc acttaccctt gcaatcccca acgcaaccag cggcgacccc
      541 gctccttatg tttacaacgt tgaagttctt gcaaatctca ttggtgtaca acctgtcaga
      601 aacggcgacc aggtgggcgt tcaagtcgtc cttgacccaa cgcgcttgcg aaatttactg
      661 gtgccgatca agaccaatgt agatcgtttg ccattgaatg caaagttcat tttcaacgat
      721 gaaacgcgag tgctggattt aatggaagat tccaaagtgg gccgcaccat ggatgtggaa
      781 gccagcatcc aggccatcaa tgacgctttg ctgcgtggag aacatacggt gaaccttgtg
      841 atcaatgaag cacagcctca ggtcacgggt acaactacag gccagcagct tggagtcacc
      901 gaattgatcc attccgagac gtcttatttt tatggttcga gtcaggaacg aatgcagaat
      961 atccaagccg cagcggagcg cttccatggt gtgctggtgg ctcctggtga aacgttctcg
     1021 atgggcgcga cgatgggcga tgtaagcctg gataacggct ttgcagaggc tctcatcatc
     1081 tatggtggtc gcacgatcaa gggcgttggg ggtggcgttt gccaggtgag caccacgctc
     1141 ttccgagccg ccttcaatgc gggcttccca attgtggaac gcacgcctca cgcgtatcgt
     1201 gtttcctatt atgaaaagat cgccagtggt gccgtggatc ctcgactggc tggcctggat
     1261 gcaactgttt acttcccact cgttgatttc aaatttacca acgattcgcc ctattggatc
     1321 ctgatggaaa cctatatggg tagtggatca ttgacctgga agttctattc caccaaagat
     1381 ggacgcactg tcacgtggga tacaactggt ccggtcaatg tggtttctgc accaccgcct
     1441 tcgtttgagc aaaacccaga gttgagtaag aatgaaatga agcaagtgga ttggtctgcc
     1501 aacggagccg atgtgactgt cacacgtaca gtctggcaaa acggtgccgt tctatatcag
     1561 gataccatct caactcacta cgaaccgtgg caggcaatct gcgaatacgg gcctgattct
     1621 aaagaccctg aaaaactggc aagagagaaa aacctttgcg gtaatccatc tgcataaatc
     1681 tgattaagtc tgtcattgcg agcgaagcgt cctcgcaatg acatagtaca tagaaatgga
     1741 gcattcatgg taagtgaaac cctcatagaa atcctgcgct gcccaaattg tgtgcgtact
     1801 accggtgggc tgctcaaact tcacaaagat acctggttca tctgccagga ctgttcgcga
     1861 aagtatccca tcgtagatga tattcctgtc atgttgatcg atgagggaga taagtgggtg
     1921 agcgtggcgg aaggcgaatt gcccgtcccg ccgcctgcaa aatagtgtga aaactgtgca
     1981 ggatttgctg aatgacttaa ccagtggcga tgaaacgcgc gcagaagatg cagtttccga
     2041 tctgatcgag aagggtgaag aagccattcc ggcgttgctg gaaatgacaa agtccggcga
     2101 tgctgacaag cgctggtggg cgctgcgtat tttggctcag tcgcctcact cacagactga
     2161 atggctgatc ccgtttctca acgatcccgc ggcggatgtg cgtcagtgtg cagcgctggg
     2221 gttgagtctc aagcctgatg aaagtgctat tgagcctctc gtccgcgcct tgagtgatga
     2281 agatacactg gttagcaacc tggcggtcaa ggcgttggtc aatctcggta aagctgcagt
     2341 tccctcctta atcgaaaatg taaaaggtgg atcacaatct tcccgtatcc ttgccttacg
     2401 tgcgcttgtg gagattcgag atcatcgtgc aattccagta atgatgcagg tcatgagtga
     2461 agagtcggct ctgctccaat attgggcaaa agaaggcttg gaacgattgg gcctggatat
     2521 ggtatatatg aagcctgtat gaacaaaact aactttttca ataacctcaa attctcgcgc
     2581 ccatccaact ggcaaatcgt tttctgggtg gtcgcggtca ttctggtcgt gggcggcttt
     2641 ttctttgtgc gcggactggt cacttgctgg atcattacgc cgttacccgg ttcacctccc
     2701 tctacctgcg gaactgtgac ttcagggaat aatggacctg tgattaccac agaggatggc
     2761 actcctgctc ccagtgtgga ggatttaccg cctccgattg tggaagtgcc tgcgtcagat
     2821 ttgcctcccg catgggatgg tgcaagtcgt atcacagtcc tcatcatcgg tctggatgct
     2881 cgtgatctcg ataccagcgc gccgcgctca gatacgatga ttctgctgac gattgatccg
     2941 ttgtcgaaga ctgcaggcat attatccgtt ccacgagata tgtgggtcaa cattcctggt
     3001 tttggctata gtcgtatcaa tactgcttat tcgtccggtg aagggaatcg gttacctggc
     3061 ggtggaccgg agttggcgag gaagacggtc gagcaattca tcggggtgcc cattcaatat
     3121 tatgcccagg tggatttcaa tacctttatc caattcatcg atcggattgg tggtatcgat
     3181 atccataata cagaggagtt gcgccttgac cgcatcggtt caggaaatga taagatcaag
     3241 ctgacctgtt gtgggatccg ccatttacct ggtgaggccg ctctggcata tgctcgccac
     3301 cgtaaaggag gggaaggaga tttcacccgc gcacaacgcc agcaaaaggt gatcatcgcc
     3361 attcgagata aagtatttga ccccgcaaac ttccccacat tgatccccca ggccgatgat
     3421 ttttataatg aattttcagc gggtatccgc accaatatgc cctttgatgt tgccattcga
     3481 ttggcagttc tcgccaggga aattccagtg gaaaacatca agcgcggcgc gatcgatacc
     3541 acaatggtgg cttttgataa tgtggttctg ggcggtcagg atgcggcgat catgaagccc
     3601 ctgcctgata agatccgcgt cttacgtgat gagattttca catcgggcgg ggcgatcagt
     3661 ccgctggcgg ctggtggaga cccccttacg ctagcaaaag ccgataccgc acggattcgc
     3721 ctgctgaacg gctccttctc gcccggactg gaaaccacta ctggaaatta tttcatttcg
     3781 cagggtctgg ctgtgactga agttggcccc gccgatcgcg cttatgatcg cacgaccatt
     3841 gtgttgtatt cgccaaagct gtatacgttg aaatatttcc agacgttatt tgccattgat
     3901 tcaaacgcct tgatcttaat acgacctgac cccagttcaa ccgtagatgt agaagtaagg
     3961 ctagggaacg actgggcaaa tagtaatccg ataccgtaaa caactctggc atcttagccc
     4021 tctcccatca gccctttcat ggtgcttcgc aaggagaggg taatttattt aaggctgttg
     4081 gaaggtgagt tttattcccc cgaaaaatgc ttctccactg ggtgtttggc aggtgggcgc
     4141 cgttaagccg gaagcgatgt cactgccagc gcggagctga accgtgtagg tgatatcggg
     4201 cgtcatgatg taatctgcat aaccattgcc aagctcaggc ttgagacctg taaaaaagtt
     4261 ttccgcaccg ccctcccagg taatcaagat ttcgataccg ggcatttgcc ttcgattgga
     4321 gtttaggatc aatacctgta acaatccgtc gggaagattt gggtcgcaga ccgagtcctg
     4381 cccggtcagc ccaaagggtg caccaaaagt tgcaaccggc gtgcgggttg gacgaggtgt
     4441 agctgccaca ggtgtatcca atggcgtttc aacggacgca ggggtttctg ttaattcaag
     4501 cggcggctca tcagatggca gggctgccgt tgaggtcggc gggctggatg tgctgtctac
     4561 agtttcagag accgccgttt ctgtatctgt actgcgggga ggattgatgc cattttggtc
     4621 aagcgcagcc gcaagggcaa caacttgatc tgcctcttcg aatgaaactc cacccgttcc
     4681 gctcgcacgc atccgttggg cctgggcgtt cagggcttcg atgggattat tgtcgcccag
     4741 gagggccagg cgtgcctgtg cgcgcgtgag atcgcccgtg gctgcgtaag cggccgcga
//
LOCUS       DASTJP010000269         6100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1185414, whole genome shotgun sequence.
ACCESSION   DASTJP010000269 DASTJP010000000
VERSION     DASTJP010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185414"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="VFQ23_08135"
     CDS             complement(<1..2079)
                     /locus_tag="VFQ23_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906595.1"
                     /translation="MAQSYRPKGTVLSRVMQWLVLIYQAVAIVVFVASIIFGLNWINQ
                     PFIGGFFEQTLVLNGVNTTKTDGQWALYAEGFTLGDQLKAIGDDPITDTNDLEEALGS
                     LTVEDTIPVTINLFDGGERTANIPLQRFPRSDQISYFILPATLSFIFLIISLWIFGLR
                     RSEPAGRAFSVMTSSLAIVIGTLFDLYTTHRFTYLWTMAAALAAGAIIDLALGFPQEA
                     RVVIGRPYLRWIGYVIGVALALYAYTKLYDFQNPTAYFKPWQNIYVFVGLSGLFYFGA
                     LAYNAFFSYSPVVKNQARTILYGSLVAFGPIVFWLLYSSLLNIIDKNRVVAFSPYFFL
                     PTVLFPLVNGYVILRFRLLQTDYWLRQGTVYSILTVLVVIMYGLLVSGLGLILPMPSD
                     NPYLVGALVFIIAIFLEPIRTRLQTLVDSTFFRGQRAFDERLRIFSHQLTNAPDLNTI
                     SRVLREQIGSSLVPDRMHVYTYDSVNDQYAALNDENNRPTSDVRFSSTGPLVQYFQKE
                     SIPLYLDTINPPSLLKGDEARLSLLGARLFVALDGDERPVGWLALGNPLSGSMYSPKD
                     LDFLNSLADQASVAISRVQTVADLERRVQEMNALTRVSQGVNITPTFDDILELIFAQA
                     TQIIPSSLFHITLYNKAANYFYYAFCVDDNDRIHTRENLPLPSSLGLGSEIIRKGRQI
                     LTQDYTRECQS"
     gene            complement(2221..4146)
                     /gene="gyrB"
                     /locus_tag="VFQ23_08140"
     CDS             complement(2221..4146)
                     /gene="gyrB"
                     /locus_tag="VFQ23_08140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET9906596.1"
                     /translation="MAKENTINYDVSSIQALEGIEHVRKRPGMYVGGTDIKALHHLVY
                     EVVDNAIDEALAGFCTRINITIHEDSSVTIEDNGRGIPVGPHPTKKDAKGHPMETVDV
                     VMTVIGAGGKFGGGGYKVSGGLHGVGVSAVNALSEWMVTEVKRDGKWWQQNYKRGVPQ
                     GAIKQIGKVKDETGTKQTFRFDKQIFTEDIDFRFDTLVQRFREMAFVTRGVRIEFVDE
                     RNNHEMTFYFEGGLTSFVRYLNRNRENLHPVVHVEKDIESIGIEAAIQYTDAYTESVY
                     SFANTINTMDGGTHLTGLRSAITRVINDYARKNSLLKDADPNFSGDDTREGLTAIVSV
                     KHPDPQFESQTKVKLMNPEAQTYVTQVVGEAFNTFLEENPQAAKAIIAKCLTSARARD
                     AAKKARELVIRKSALESLTLPGKLADCSERDSSKTELYIVEGDSAGGSAKQGRDRHFQ
                     AILPLRGKIMNTERARLDKILSSNEIKALISALGTGIGDNFDVEGLRYGRVIIMTDAD
                     VDGSHIRTLLLTFFFRYMPQLIEDGHLYIAQPPLFRVAYKNQIKYAYTEKEKDKMVKE
                     IGEKASLQRYKGLGEMNPTQLWETTMNPENRTLLLVTVEDAAESDRTFDMLMGDAVDP
                     RRKFIQTHAKAVRNLDI"
     gene            complement(4210..5223)
                     /locus_tag="VFQ23_08145"
     CDS             complement(4210..5223)
                     /locus_tag="VFQ23_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906597.1"
                     /translation="MDSQKLESLPPPPGVIVSLRAGFDAVANHVWLILLPLVLDIFLW
                     LGPRLSVDGLLRPFVRYMFAQARRGVGSAEMDQFLEAQTIFLDGLQSFNLLSLISKFR
                     TFPIGISSLLAEKMPVESPIGEQSIVQISSVPLLLLIAMLLTAIGWVIGGLYFRWVSG
                     TTLGQQETGISLLRAIVQTLILSVCWFIGLMVVFLPIMFVLTLLALINPILANIAMFV
                     ILLLAFWLIVPFFFMPHGIFARGQNAFLSILTSLRMARFTFPSSGMFVISVLVLSQGL
                     NYLWSVPPDNSWMTLVGIAGHAFITTAVLAASFVYYRDMNVWLQTVFERWQKAHTTPK
                     QHA"
     gene            5282..6094
                     /locus_tag="VFQ23_08150"
     CDS             5282..6094
                     /locus_tag="VFQ23_08150"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906598.1"
                     /translation="MNEQRYLPDSNRIGVLTSTVLLALALTRLIQAPEFNLDVQLPGF
                     FLTLPLNISTAMSLLTAGLTATGMDWLLRSHPSWNNQPTFQSWLLPTLTTFVIGVPLS
                     ILPDGAAWWTGFAIGGAFLFFVFLAEYIVVDSGAPYYAVAMAGLTAISYTLFFILAFA
                     LRYGGVRLYILIPALFLAAALASLRILRLRLSGIWEVAWAIGIAFACVQIAAGLHYWP
                     LSPIQFGLMLIGPLYALTNLAINIGDGLPASRASLEPAIVSALCWGLAIVIR"
BASE COUNT         1589 a         1579 c         1455 g         1477 t
ORIGIN      
        1 ggattgacac tcgcgcgtgt aatcctgcgt caggatttgt cgtcctttac ggatgatttc
       61 agagcctaat cccaaactgg atggcaacgg cagattttca cgcgtatgga tacgatcgtt
      121 atcatcaaca caaaaggcat aatagaaata attcgctgcc ttgttataca gggtgatatg
      181 aaacagggaa gacgggatga tctgggttgc ctgggcaaag atcaactcca gaatatcgtc
      241 aaacgtaggt gtgatattca caccttgcga aacacgtgtg agtgcgttca tttcctgcac
      301 gcggcgttcc aggtcagcta ccgtttgtac acgcgagatc gcgacggacg cctgatccgc
      361 aaggctgttc aaaaaatcga gatccttggg tgaatacata ctgccagaaa gcggattccc
      421 cagggccagc caaccaacag ggcgttcatc tccatccaaa gcgacgaaga gacgcgcacc
      481 cagcagggat agacgcgctt cgtcaccctt taggagagac ggtggattaa tggtatccag
      541 atagagcgga atgctttctt tttgaaaata ctggacaagt gggcctgttg aggaaaaacg
      601 aacatcgctg gtcggacggt tgttctcatc attcagagct gcatattgat cgttgaccga
      661 gtcatacgtg taaacatgca tccgatcggg aaccaggctg ctgccgatct gctcgcgtaa
      721 cacgcggctg atcgtattca aatctggagc attggtcagt tgatggctaa agatgcgaag
      781 gcgttcatcg aacgcgcgct ggccgcggaa gaatgtgctg tccaccaggg tctgcaaacg
      841 tgttcgtatc ggttcgagaa aaattgcaat aataaaaacc agagcgccca ccaaataggg
      901 attatcggaa ggcatgggga gtatcagtcc cagcccgctc accaacaatc cgtacatgat
      961 gaccaccaga accgtcaata ttgaatagac cgtgccctgc cgtaaccagt aatctgtttg
     1021 caggagacgg aatcgtaaaa tcacatatcc attcaccagg ggaaagagta cagttggcag
     1081 gaagaaatat ggactaaatg caactactcg gtttttatca ataatgttca ataacgatga
     1141 atacaataac cagaaaacaa tcggaccaaa ggcgaccaac gacccataga gaatcgtccg
     1201 ggcttgattc ttaacgacgg gtgaataaga aaagaaagcg ttgtacgcaa gcgcgccaaa
     1261 gtagaataac ccggataagc cgacgaatac ataaatattc tgccagggtt tgaaataagc
     1321 cgttgggttc tggaagtcgt ataactttgt ataggcatag agcgccaggg caacaccgat
     1381 cacatatccg atccagcgca gataagggcg accgatcacg acgcgtgctt cctgtggaaa
     1441 gccaagagcc agatcaataa ttgcaccagc agccagtgcg gcagccatcg tccagaggta
     1501 ggtaaaacga tgagtagtgt atagatcgaa cagcgtgcca attacaatcg ccagtgagga
     1561 agtcatcaca gaaaaggcgc ggcctgccgg ctcacttcga cgcaatccga aaatccacaa
     1621 actaatgatg aggaaaataa agcttagcgt cgctggcagg atgaaatacg aaatttgatc
     1681 ggaacgagga aaccgctgta atggaatatt ggccgttctt tccccaccat cgaataaatt
     1741 gatcgtgacc gggatagtat cttccacagt cagcgaaccc agcgcctctt ccaggtcgtt
     1801 tgtgtcagta atcggatcat cgccaatcgc cttgagctgg tcgcccaacg tgaagccttc
     1861 ggcatagaga gcccactgtc catccgtttt ggttgtattc acgccgttca aaacaagagt
     1921 ctgctcgaaa aatccgccaa tgaaaggttg gtttatccag ttcaagccaa aaattatgga
     1981 cgcgacaaaa accactatgg ccactgcctg atagatcagg acaagccatt gcatcactct
     2041 acttaagact gtgcctttag gacgatacga ttgtgccatg cttccacagg aaaaaactaa
     2101 aaaacgcaga acctcatgga attttacact acatccccac cgaccaacta tggtactttc
     2161 ataacaaaga agaaaggcgg agtgtcctgt gagaaggatc actccgcctt gtgcttggaa
     2221 ctaaatgtcc aggtttcgaa ccgctttcgc gtgtgtctgg ataaatttac ggcgaggatc
     2281 caccgcgtca cccatcagca tatcaaacgt tctatcggat tcggctgcat cttctaccgt
     2341 gaccagcagc aacgtcctgt tttcggggtt catggtcgtc tcccacagct gggtcgggtt
     2401 catttcaccc agacctttgt atcgctggag gcttgccttt tcgcctattt ccttaaccat
     2461 cttatctttt tctttctcgg tataggcata tttaatttgg tttttgtagg caacccgaaa
     2521 cagagggggt tgagcaatat acaagtgacc atcttcgatc aactgcggca tatagcggaa
     2581 gaagaaggtt aacaacaggg tgcgaatgtg actgccgtct acgtctgcat cggtcatgat
     2641 gatcacgcgt ccgtagcgca gaccctcgac atcaaagttg tcgccaatgc cagtgcccaa
     2701 tgctgagatc aacgccttga tttcgttact cgacaaaatc ttatccaggc gagcgcgttc
     2761 ggtgttcatg atcttgccgc gcaatggaag aatcgcctga aaatgccggt cgcgtccctg
     2821 tttggcagag ccgcctgccg agtcaccttc cacgatgtag agctcagtct tggaagagtc
     2881 gcgctcggag caatctgcca gtttgccagg tagcgtcagc gattcaagag ccgattttcg
     2941 gatcaccagt tcacgggcct tttttgcggc atcgcgggcg cgagcagaag tcagacactt
     3001 cgcaatgatc gccttggcgg cctgtgggtt ttcctcaaga aacgtgttga atgcctcacc
     3061 cactacctgt gtgacatagg tctgtgcctc cggattcatc agcttgacct tggtctgcga
     3121 ctcaaactgc gggtccggat gtttgacgga gacaatcgcc gtgagtcctt cacgtgtatc
     3181 atcacccgag aagttcgggt ctgcatcctt aagcaaactg ttcttacgag cataatcatt
     3241 gattacacgc gtaatcgcag agcgcaaacc agtcaaatgc gtaccgccgt ccatggtatt
     3301 gatggtattt gcgaaggaat aaacagattc ggtatacgca tctgtatact ggatagcagc
     3361 ttcaatcccg atggactcga tgtccttttc tacatgtaca accggatgca ggttctcgcg
     3421 gttgcgattc aggtagcgta caaaagaggt cagcccacct tcgaaataaa aggtcatctc
     3481 atgattatta cgctcatcca caaactcgat acgtaccccg cgcgtaacaa atgccatttc
     3541 gcggaaacgc tggaccaggg tatcgaaacg aaaatcaata tcttctgtga agatttgctt
     3601 gtcaaatcga aaagtttgct tggtgcccgt ttcgtccttt accttgccaa tctgcttgat
     3661 ggccccttga ggaacgccgc gcttatagtt ttgttgccac catttaccat ctcgcttgac
     3721 ttcggtcacc atccattcag aaagtgcatt gaccgcgctg acacctacac catgcaaacc
     3781 accggagact ttgtatccgc cgcctccgaa tttaccgcct gcaccgatca cggtcataac
     3841 cacatccacg gtctccatcg gatgcccctt tgcatccttt ttcgtggggt gcggcccgac
     3901 cggaatacca cgcccgttat cttcaatggt cacactgcta tcttcgtgaa tcgtaatgtt
     3961 aattctcgta caaaaacctg cgagcgcttc atcgattgca ttatccacaa cctcatacac
     4021 cagatggtgc aaggctttaa tatctgtacc acccacgtac atgcccgggc gtttgcgtac
     4081 atgctcaatt ccttccagtg cctgaatgga actgacatca taattaatcg tattttcttt
     4141 tgccacaaaa tcctccaatg gtttcctttc cctctgggaa aggagctacg ttaaggtgtg
     4201 tatttttctt cacgcatgct gttttggcgt cgtgtgtgct ttttgccacc gttcgaaaac
     4261 agtttgtaac caaacattca tatcgcgata gtacacgaag cttgccgcca ggactgccgt
     4321 tgtaataaag gcgtgcccgg cgattccaac aagtgtcatc cacgaattgt cgggagggac
     4381 actccataaa taattcaatc cctgcgaaag gaccaataca ctgatgacaa acattccgct
     4441 cgaagggaag gtgaaacgcg ccatccgtaa gctggtcagt atcgagagaa aggcattctg
     4501 cccacgggca aagatcccgt gtggcataaa gaaaaatggg acaatcaacc agaatgccag
     4561 aaggaggatc acaaacatcg caatattggc caggatcgga ttgatcaacg ccagtaacgt
     4621 cagaacaaac atgattggca gaaaaaccac catcaaaccg atgaaccaac agacagacaa
     4681 aatcagggtt tgaacgatgg cccgcagcag gctgatccct gtttcctgct gacctaaggt
     4741 tgttcccgac acccagcgaa agtacaaacc gccgattacc cagccaatcg cggtcaatag
     4801 catggcaatg agcaataaaa gaggtacaga tgaaatctgc acaatgcttt gctcgccaat
     4861 cggcgattcc acaggcattt tctctgccag caggctcgaa ataccaattg gaaaggtacg
     4921 aaatttactg atcaggctta gtaaattgaa gctctgcagc ccgtccagaa aaatcgtctg
     4981 agcctccagg aactgatcca tctcagccga accgacgcct cttctcgcct gggcaaacat
     5041 gtaccgcaca aaaggacgaa gcaaaccatc cacactcaag cggggtccta gccataggaa
     5101 aatatccagg accaggggta acaatatcag ccagacatgg ttggcaaccg catcaaaacc
     5161 ggctctgagc gaaacgatca cacccggagg tggagggagc gattcgagtt tctgcgagtc
     5221 cataaccaat tgattctacc atgtcttggc attcaataat gaaggcaggt acaatgcgtt
     5281 aatgaatgag caaaggtatt tgcccgattc aaaccgcatc ggagtactca cctcgaccgt
     5341 actgctggca ttggcgctca cgcgcctcat ccaggcgccc gagtttaatc tcgacgtgca
     5401 attacccggc ttttttctca cccttccatt gaatatttcc acagccatga gtctgcttac
     5461 tgccgggctt actgccacag gtatggactg gcttttgcgg agtcacccgt cctggaataa
     5521 tcagccaacc tttcaatcgt ggttgctgcc gactctcacc acatttgtga tcggtgttcc
     5581 actttccatt ctcccggacg gcgctgcctg gtggactggc tttgcgattg ggggcgcatt
     5641 tctcttcttt gtctttcttg ccgaatatat tgttgtggat tccggggcgc cctactatgc
     5701 cgtcgctatg gcagggctga ctgctatttc ctacactctc ttctttatat tggcctttgc
     5761 gctacgctat ggtggtgtgc gcctctacat cttgattcct gccttgtttc ttgctgcagc
     5821 gctggccagc cttcgaattt tgcgtttgcg cctgagcggc atctgggaag tggcctgggc
     5881 tattggtatc gcgttcgcct gtgtccaaat tgcggccggg ctgcattatt ggcccctttc
     5941 cccgatccag tttggattga tgctgatcgg tccgttgtac gcactcacaa acctggcgat
     6001 aaatatcgga gatggcttgc ctgcctcacg cgcttccctt gagccagcaa tcgtcagcgc
     6061 attatgctgg ggactggcaa ttgtgattcg ataagagcat
//
LOCUS       DASTJP010000270         6763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1060433, whole genome shotgun sequence.
ACCESSION   DASTJP010000270 DASTJP010000000
VERSION     DASTJP010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060433"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFQ23_08155"
     CDS             <1..327
                     /locus_tag="VFQ23_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET9906599.1"
                     /translation="KPARVGGLSQAIAIHDLCVERNVPVWCGGMLETGVGRASNLALA
                     SLSGFVLPGDISASDRYYQRDITNERFVLNDDSTINVPTGPGLGVTIDEDALKKFTIS
                     EITLKS"
     gene            390..2090
                     /locus_tag="VFQ23_08160"
     CDS             390..2090
                     /locus_tag="VFQ23_08160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET9906600.1"
                     /translation="MIKPFARLFILITVLASACAPSNTSNLQPTAFQSAFISPTSPAE
                     NVAVEMSTFTPESAGSFNSDAIPPALRAQADELNVSLDISSSVSTNDDQIEWLHALVA
                     PFPTVIDGVTFDELHIAWTEGTAPAPFDGIPLLIDESTLAAFTALWDAPADGSVKVVP
                     ADQILDTAWSEMPSWAIIPFESLEPKWKVLTIDGQSPIRKGFDVSTYPLVVNFTLQPS
                     NSQLSSNYDPSKLTTVIMTGVTALVRATALTMELKGSTYPGEQVRDILREADITHVSN
                     EIPFFTGCTYPKPDQVALVFCSDPKYMDLLTDVGTDVVELTGNHFADRGPAGMLETIE
                     IYNANGLPYFGGGTDLQDSLEPALFEVNGNKIAFIGCNAPDVNRFPTATDFQPGAAPC
                     DFPYLTQKINQLKAEGYVVISTFQWYESYDAYPSPNQKNDFRLMAESGASIVSGSQAH
                     YAQAMEFHNDSFIHYGLGNLFFDQMGDQDWMPRGIRREFFDRYVIYDGKLISVELITA
                     MLEDYARPRIMTEEERAGFLQEYFAYSGWIPLVSTPTPTITPTLTPMPIPPVDLTPGP
                     "
     gene            2230..2865
                     /locus_tag="VFQ23_08165"
     CDS             2230..2865
                     /locus_tag="VFQ23_08165"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9906601.1"
                     /translation="MTIYEISLSISPTLPTWPGDPALELELIESMDKGAHANVTRMSA
                     AVHLGTHVDAPHHFLNDGRTVESLPLDVLTGPCYVTQLPDGVEAITAEVLDRTEITSE
                     MKRVLFGTRNSHLWAKGEAAFQTDFVAITEDGAEWLVEHGIQLVGVDYLSVAPYGDSV
                     PTHTVLLQAGIVCVEGLNLSQVMRGFYDLYCLPLKITGSDGAPARAILIQR"
     gene            complement(2932..4893)
                     /gene="acs"
                     /locus_tag="VFQ23_08170"
     CDS             complement(2932..4893)
                     /gene="acs"
                     /locus_tag="VFQ23_08170"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET9906602.1"
                     /translation="MAKSTTVKKPSTKKTTINTKAKKKPVRMHGEVYYPPKHVVEQAR
                     LKDWDKLAKSARKDLKGFWESEAKELEWFSKWKKVLDNSKKPFYKWFTGAKVNIVHNA
                     VDRHLKTYRKNKLALIWESEDGKDHRTYSYYALNREVTRMANIIKSMGVKKGDRVTIY
                     MGRVPEIVFAMLACAKIGAIHSVVFGGFSVDSLQGRIEDSTSNLVITCDGSFQNGKVV
                     QLKQIVDDALKRCPTVQNVIVVKRVGNEVNMETGRDHWYHDLLEQPIAEGKCETEQLD
                     SEDPLFILYTSGSTGKPKAILHTHGGYMVGTYTTLKYVFDIRDDDRWWCTADPGWITG
                     HSYIVYGPMLNGATSFLFEGGPTYPEPNRWWRVIERYGITIFYTAPTAIRGLMRFGDS
                     WPEKHDLSSLRLLGSVGEPINPEAWKWYHRVIGKEKLPIMDTWWQTETGAFMITPTPV
                     VPLKPGSGTRPFFGQEAEIVDEQGKPVPDDTEGYLVLKNPWPAMLRTIYGDPDRYVNQ
                     YWSKYPGKYTTGDSAKRDRDGYFWIIGRVDDVIKVSGHRLGTAEVESALVSHPAVAEA
                     AAIGLPHDVKGQAIYTFVLLRAGQSASPELAEELRQHVSKHMGPIARPEEVRFVDKLP
                     KTRSGKIMRRVLKARAQGLPEGDISTLEE"
     gene            complement(4939..6243)
                     /locus_tag="VFQ23_08175"
     CDS             complement(4939..6243)
                     /locus_tag="VFQ23_08175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9906603.1"
                     /translation="MPKQQIYWHTTVQMPDDLNLTPLPEKVDVAVIGGGFTGLSAART
                     LAKRGAKVAVLEANTIGWGASSRNGGMTLTGLKPSMHTVIKKYGRDVARRLFQCSLES
                     VDTVEQVVKEENIDCGFARRGHLLVANKPNQFDALQQEVEFMEKEFNHKVRVVSREGQ
                     REEIGTDLYYGALIDEVSGGLNPAQYVTGLARAAERAGATLHARARVNRLERASSPLG
                     ASGNRFLLETERGTVHAENVMVGTSGYTGSVTKKLQKKIVPIGSFIIATERLSDELAN
                     ELSPKNRMIFDYKHYLNYFRLWDNRMIFGGRAAFFPENKNTIARSAEILRREMIQVYP
                     QLKDIKIEYVWGGTLDFAFDMMTHVGEVDGIYYSLGYAGHGVAMASYLGKTVAEAMLA
                     GNIKDHPFASFSFPGAPLGLYNGFPWFLPFAGAWHKILDWIE"
     gene            complement(6277..>6763)
                     /locus_tag="VFQ23_08180"
     CDS             complement(6277..>6763)
                     /locus_tag="VFQ23_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906604.1"
                     /translation="GALLLAGRKLFWLFVGAIGFVTGLQFATNFWQGPDNLALVLGLI
                     VGVIFALLAVFLQTIAIGIAGFFAGGYVLTALAGMLGIDTSATFWIIYVIGGIIGLAV
                     VIYLFDWAIITLSSLAGASLLLQALFPQSSTGGVIFAVLFIIGVAVQGSVLRSERPAK
                     R"
BASE COUNT         1582 a         1807 c         1658 g         1716 t
ORIGIN      
        1 aagccggcgc gagtgggcgg gttgagccag gcaattgcga ttcatgatct ttgtgtggaa
       61 cggaatgttc ccgtctggtg tggtggcatg ctggagacag gtgtgggacg cgcctcgaat
      121 cttgccctgg cttccctttc cggttttgtc ctaccaggag atatttctgc gtctgatcgc
      181 tattatcaga gggatatcac caatgaacga tttgttttga acgacgacag cacgatcaac
      241 gtaccgactg gcccgggttt aggggtgacg attgacgaag atgcattgaa gaaatttact
      301 atctcagaga ttactctgaa atcataaaaa taaatggttt atgagttagg ggaaagcgat
      361 gttggcagct ttcagtataa tcagcagtaa tgataaagcc tttcgcacgt ttatttattc
      421 tgatcactgt tttagcttca gcatgtgcac ccagtaacac ctcaaatctc cagccaacag
      481 cttttcagtc agcttttatt tcaccgacat ctccggcgga gaatgtcgcg gttgagatgt
      541 ccaccttcac gcctgagtct gccggctcat ttaacagcga tgctattccg ccggcgctgc
      601 gcgcacaggc ggatgagttg aatgtgtcac tcgatatttc ttcatcagta tcgacaaatg
      661 atgatcaaat tgaatggctt catgcattag ttgccccctt cccgactgtc atagacgggg
      721 tgacatttga tgaactgcat atcgcctgga cggaaggcac agctcccgcg ccgttcgacg
      781 gaatcccatt attgatagac gaatctacat tggctgcgtt caccgcgctt tgggacgcgc
      841 cagcagatgg atcggtaaaa gtggtgcctg cggaccaaat actggataca gcatggtcag
      901 agatgccatc ctgggcgatc atcccgtttg aatcgctgga accaaaatgg aaggttttga
      961 ccattgatgg gcaatcaccg attcgcaagg ggtttgatgt atcaacctac cctcttgttg
     1021 ttaatttcac acttcaacct tcaaacagcc aactctcgtc aaactatgac ccgtcgaaac
     1081 ttacgacagt cattatgacg ggtgtcactg cccttgtgcg cgctacggcg ttgacgatgg
     1141 aattgaaagg ctcaacgtat ccgggtgagc aggtgcgtga catcctgcgt gaggcggata
     1201 tcacgcatgt cagtaatgag attccgttct ttactggctg cacctatccc aaacctgacc
     1261 aggttgcgct cgtgttttgc agtgatccaa aatatatgga cttgcttacc gatgttggaa
     1321 ccgatgtggt tgagttgacc ggcaatcatt ttgcggaccg cggtcctgca ggcatgctgg
     1381 aaacgattga aatctataat gccaatggtc ttccttattt cggtggcggc accgatttgc
     1441 aggattccct ggagccagcc ttattcgaag tcaatggcaa taagatcgcg tttatcggct
     1501 gcaacgcacc cgatgtcaat cgcttcccaa ccgcgaccga ttttcagcct ggcgccgcgc
     1561 cgtgcgattt tccttatctt acacaaaaga tcaatcagtt gaaggctgag gggtatgtag
     1621 tcatttctac gtttcagtgg tatgagagtt atgatgccta tcccagcccg aaccagaaaa
     1681 atgacttccg cttaatggca gagtctggcg cgtcgattgt cagcggcagt caggcccatt
     1741 acgcgcaggc gatggaattt cataacgact cgttcatcca ctacggactt ggcaatctgt
     1801 tctttgatca gatgggcgat caggattgga tgccgcgcgg cattcgccga gaattctttg
     1861 atcggtatgt gatttacgat ggaaagctga ttagcgttga attgatcacg gccatgctcg
     1921 aagattatgc ccgcccgcgt atcatgaccg aggaagaacg cgcgggattc ctgcaggaat
     1981 attttgccta cagcggatgg attccattgg tctcaactcc aactccgacg atcacgccca
     2041 ccttaacgcc tatgcctatt ccgccggttg atttgacgcc aggtccataa gtatattcgc
     2101 gttgagcgga gccgcatgtg tgtgcggcac agtcgaaacg ccgttatgta ctaagcccgc
     2161 ttcgtctatg gctcctctac ttgccctcgg agcctccgct caacgcgata taaataaaag
     2221 gagtttccca tgacaatcta cgaaatctcc ctaagcattt ctcccacact gcccacctgg
     2281 ccgggtgacc ccgcgctcga actggagctg attgaatcga tggacaaggg cgcgcatgcc
     2341 aatgtcacgc ggatgagtgc cgctgtccat ctggggacac atgtggacgc gcctcatcac
     2401 tttctgaacg atggccgcac cgtggagagt cttccactcg atgtactgac cggtccctgt
     2461 tatgtgacac aactcccgga tggggtggaa gcgatcaccg ccgaagtatt agatcggaca
     2521 gagattacct ctgaaatgaa acgcgtcctg tttggcacgc gcaattctca tctctgggcg
     2581 aaaggcgaag cggccttcca aacggacttt gtcgccatca cagaagacgg agctgaatgg
     2641 ctggtggagc atgggattca actcgttggg gtggattatc tctccgtcgc gccctatgga
     2701 gactccgtgc ccacacatac ggtattgcta caagcgggga ttgtatgtgt tgaaggactg
     2761 aacctttcac aagtgatgcg cggattttac gatctttact gtctaccttt aaaaatcact
     2821 ggttcagatg gtgcccccgc aagagcgatt ctgattcagc gctgataaaa aaaatcaccc
     2881 cgcgctcgaa ttgagtcgcg gagtgatttc taatcgctac tatctgacgg actattcctc
     2941 taaggtcgaa atatcgcctt ctggcaatcc ttgtgctcga gctttcaaaa cgcggcgcat
     3001 gatcttgccg gaacgcgtct tgggcagttt gtctacgaag cgcacttctt cggggcgtgc
     3061 gatgggtccc atgtgttttg aaacatgttg gcgcaattct tctgccagtt ctggggacgc
     3121 actctgccca gcccgcaaca aaacgaatgt gtaaatggcc tgtcctttga catcgtgtgg
     3181 aagccctatg gccgcggctt ctgcaactgc gggatgactc accagcgcgg attcaacttc
     3241 tgcagtgccc agccgatgcc cggaaacttt gatcacatcg tccacacgac caataatcca
     3301 aaagtaacca tcccggtcgc gtttggcaga gtcaccggtg gtgtatttgc cgggatattt
     3361 actccaatat tgattgacat aacggtcagg gtcgccataa attgtccgca gcatcgcggg
     3421 ccagggattc ttcaggacaa gatagccttc cgtgtcatct gggacgggtt tcccttgttc
     3481 atccacgatc tcagcctcct gcccaaaaaa tgggcgtgta cccgacccgg gcttgagtgg
     3541 cacgacgggc gttggcgtga tcatgaacgc gcccgtctcg gtctgccacc acgtgtccat
     3601 gatcgggagc ttttccttgc cgatgacgcg gtgataccac ttccacgcct cggggttgat
     3661 cggctcaccc acggaaccga gcaatcgcag ggaggaaaga tcatgcttct caggccagga
     3721 atcgccaaac cgcataagtc cacggatggc cgttggagcc gtgtagaaga tcgtgatccc
     3781 atatcgttcg atgacacgcc accatcggtt cggttctgga taggttggtc cgccttcaaa
     3841 gaggaaggag gtcgcgccgt tgagcatggg tccgtacaca atataggagt gacccgtgat
     3901 ccagcctggg tcagccgtgc accaccagcg gtcgtcatcg cggatatcga acacatattt
     3961 aagcgtcgta tacgtgccaa ccatgtatcc accatgtgta tgaaggatag cttttggttt
     4021 gccggtcgag ccactcgtat aaagaataaa aagaggatcc tcagaatcca gttgctcagt
     4081 ttcgcatttg ccttcggcaa tcggctgttc gagcaaatcg tgataccagt ggtctcgccc
     4141 cgtttccata ttgacctcgt tgcccacgcg cttaacaacg attacattct gtacggtcgg
     4201 acaacgttta agcgcatcat ccacgatttg cttgagttga acaacttttc cgttctggaa
     4261 cgaaccgtcg cacgtgatga ccaggttcga cgtgctatct tcaattcgac cttgaagtga
     4321 atccacacta aagccgccga acaccaccga atggatggcc ccgatctttg cgcaggccag
     4381 catggcaaat acaatttcgg gcacacgccc catataaatc gtgacgcggt cgcctttctt
     4441 gacacccatg cttttgataa tgtttgccat gcgcgtcact tcacggttca gcgcgtaata
     4501 ggagtaggtg cgatgatcct tgccatcttc gctctcccag atgagcgcaa gcttattctt
     4561 acgatatgtc tttaagtgac ggtcaaccgc attatgaaca atgttaactt ttgccccagt
     4621 gaaccatttg taaaatggct tctttgaatt gtccagaact tttttccatt tgctgaacca
     4681 ttccagctct tttgcttcgc tttcccaaaa cccttttaag tctttgcggg ccgattttgc
     4741 aagtttgtcc cagtctttca gccgtgcctg ctccaccaca tgtttcggcg ggtagtacac
     4801 ctcgccatgc attcgtacag gtttcttttt ggcttttgta tttattgtgg ttttctttgt
     4861 agagggtttc ttaaccgttg tactcttcgc catgacaatc tcctgatcgt gggatgcaat
     4921 aaaacaattt tactccgatt actctatcca atccaatatc ttgtgccatg cgcccgcaaa
     4981 gggcaaaaac catggaaacc cgttatacag tcccagcggc gcgccaggaa acgagaatga
     5041 ggcaaacggg tgatccttga tgtttcccgc gagcatcgct tccgcgaccg tctttcccag
     5101 gtagcttgcc atcgctacgc cgtgacctgc atagcccagc gaataataga ttccatccac
     5161 ctcgcccacg tgggtcatca tatcgaaggc aaaatccaat gtgcctcccc aaacgtattc
     5221 gatcttgata tctttcaatt gcggatagac ctggatcatc tcgcgccgca atatctcagc
     5281 actgcgtgcg attgtattct tattttcagg gaagaatgct gcacgaccgc caaagatcat
     5341 gcggttatcc cacaaacgaa aataattcaa ataatgtttg taatcgaaga tcatgcggtt
     5401 cttcgggctg agttcgttgg ctaattcatc cgagagtctt tccgttgcaa tgataaagga
     5461 accgatcggg acgatcttct tttgtaattt ctttgtgaca ctccctgtat aaccagacgt
     5521 gcctaccatg acattttctg catgaactgt gccgcgttcc gtttcgagca ggaaccggtt
     5581 tccactcgca ccgagcggag acgaagcgcg ctcaagtctg ttgacgcggg cccgcgcgtg
     5641 gagcgtcgcc ccggcccttt ctgccgcgcg cgcgagtccc gtcacgtatt gcgccgggtt
     5701 gagcccgcca ctgacttcgt cgatcaacgc gccataataa agatcggtgc cgatctcttc
     5761 acgttgtcct tcgcgagaga caacccgtac cttgtggttg aactcttttt ccatgaattc
     5821 cacttcctgc tggagggcgt cgaattgatt cggtttgttg gcaaccagta aatgtccacg
     5881 gcgcgcgaag ccgcaatcaa tattttcctc tttcaccact tgttccacgg tatccacgga
     5941 ttccagcgaa cattgaaaca gccttcgggc aacatcgcgc ccatacttct tgatcaccgt
     6001 gtgcatggat ggcttcaacc ccgtgagtgt catcccacca ttccgtgaac tcgcacccca
     6061 accaatcgta ttggcttcca aaacggctac ctttgcaccg cgcttcgcca gcgtccgcgc
     6121 tgcagagaga cctgtaaaac caccgccgat gactgccaca tccacttttt cgggaagagg
     6181 tgtcagattt aagtcatcag gcatttgcac agttgtgtgc cagtagattt gttgtttggg
     6241 cattgctgtc ctctatgaat gattcaggat tttaacttac ctttttgctg gtcgctcact
     6301 acgaagtaca gagccttgaa ccgcgactcc gatgataaac agcactgcaa agatcacgcc
     6361 acccgtactg ctttgaggaa aaagggcttg aagcagcaat gaggcacccg cgagagagga
     6421 caaagtaata atcgcccaat caaagagata gatcaccacg gccagtccta taatgccccc
     6481 gataacgtaa atgatccaga aggtagcact ggtatctatc ccaagcatac ctgctagcgc
     6541 cgtcagcaca tatccgcccg cgaagaagcc ggctattccg atcgcgatgg tctgaaggaa
     6601 aacagccagt aaggcgaaga tcacaccgac gatcaaccct aaaacgagag ccagattatc
     6661 cggtccttgc cagaaatttg ttgcaaattg cagaccggta acaaacccaa ttgcaccgac
     6721 aaatagccag aatagtttgc gccctgcaag cagcaaggca cca
//
LOCUS       DASTJP010000271         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1049773, whole genome shotgun sequence.
ACCESSION   DASTJP010000271 DASTJP010000000
VERSION     DASTJP010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049773"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFQ23_08185"
     CDS             complement(<1..342)
                     /locus_tag="VFQ23_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906605.1"
                     /translation="METALPSRSLGKPGLLSDLRAFGAILQREWRIFVRYPSWIIALF
                     IWPLIFPMMYILTGRALSGPDGTGLEVFRQTTGATDFIGYIVIGTTVWMWQNIVLWDV
                     GFSLRDEQMRGT"
     gene            complement(345..1397)
                     /locus_tag="VFQ23_08190"
     CDS             complement(345..1397)
                     /locus_tag="VFQ23_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9906606.1"
                     /translation="MSDFAIEASQLVKKFPAKPGTGDKNPDHLPTTRRSRWPFGKKEP
                     KAMFTAVNGVDLQIQRGEIFGLLGPNGAGKSTTIRMLCTLLEPTSGMARVNGFDIVKQ
                     ANDVRRSLGTLLAGERSIYWKLSGRENLEYFAALYHIPQPVAKHRIEELIERMELKDR
                     ANELVEKYSTGMRQRVAIAKALLARPPILLLDEPTLGLDPQAARRLRELIAELKQEGH
                     TILLTTHYMEEADLLSDRIGIIDSGRVIALDTPEGLKHRIEQKEVIRLEVTGWHEEIA
                     EKLKSISGIENLISRQDGEADLWEVNMQAHNSRAAIPRIVEHISSNGTRLVNMNIVKP
                     SLEDVFIHLTGKALRD"
     gene            complement(1569..>2117)
                     /locus_tag="VFQ23_08195"
     CDS             complement(1569..>2117)
                     /locus_tag="VFQ23_08195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9906607.1"
                     /translation="YQQQYSTLSVYMRDVHHIDSQGYGLILSITGLEVVLFQFWISRK
                     IRRHAPFLIMMLSTIFFAIGVFMYGLVSSFAMFVVAAIIVCIGEMLFFPTSQALVAGF
                     APEDMRGRYMAISGLTWSLPAAIGPGAAGYILDNANPNLLWQIGGYICILAAIGYYAL
                     HLRLRSHPQFSPVKDEVEASPA"
BASE COUNT          549 a          535 c          514 g          519 t
ORIGIN      
        1 tgtgccgcgc atttgttcgt cacgcagcga aaaaccgaca tcccacaaaa cgatgttttg
       61 ccacatccat acggttgtgc cgatcacgat gtaaccgatg aaatcggtcg caccagtggt
      121 ttgcctgaag acttccaatc cggttccgtc aggacctgaa agcgcgcggc ctgtaaggat
      181 gtacatcatc gggaagatca agggccagat gaagagcgca ataatccagc tcggataacg
      241 aacaaaaata cgccattcgc gttgtaggat cgctccaaaa gcgcgcaggt cagataacaa
      301 cccaggcttg cccagcgaac gagagggaag agctgtttcc attcttaatc tcttaacgcc
      361 ttgccggtta gatgaatgaa cacatcttcc aacgatggct tgacaatgtt catgttgacc
      421 aatcgcgtgc cattgctact aatatgctca acaatacggg gaatcgcagc gcggctgttg
      481 tgtgcctgca tgttcacttc ccacagatcg gcttcaccgt cctggcgcga gatcagattc
      541 tcaataccgg aaatactctt cagcttttcg gcaatctcct catgccagcc agtgacttca
      601 agacggatca cttccttttg ttcaattcga tgcttgagtc cttcgggcgt atccagtgcg
      661 atgactctgc cagaatcaat gatgccaatt cgatcagaga gcagatctgc ctcttccatg
      721 tagtgggtcg tcagcaggat ggtgtgtcct tcctgcttga gttctgcgat caactcccgt
      781 aaacggcgag cagcctgagg gtccagacca agcgtaggtt cgtctaagag aagaataggt
      841 gggcgggcca gcagagcttt ggcgatggcg acgcgttgtc gcatccccgt cgaatatttt
      901 tcgacaagtt cattggcgcg gtccttgagt tccatccgtt cgatcaattc ttcgatgcga
      961 tgcttcgcta cgggttgcgg gatatgataa agtgccgcaa agtattccag gttctcgcgc
     1021 ccgctcaact tccaatagat actgcgttcg cctgccagca atgtgccaag gctgcggcgg
     1081 acatcgtttg cctgtttgac gatatcaaag ccattgacgc gcgccatgcc gctggtcggc
     1141 tccagtaagg tgcacaacat acgaatggtt gtggatttcc ccgcgccatt gggacccagt
     1201 aatccaaaga tctcaccgcg ctgaatttgt aaatctaccc cattgacggc agtaaacatc
     1261 gcctttggtt cttttttgcc aaagggccag cggctacgcc tggttgtggg gaggtggtcc
     1321 gggtttttat ccccagttcc tggctttgcc ggaaatttct tgactaattg actggcttca
     1381 atcgcgaaat cagacatcgt aggctccgtt tttttgaagt ctggattaaa aatcgtgata
     1441 aatttcatca gattttttaa ctatctgtaa aatattttca tatattacaa cttaagtttc
     1501 aatatgtcaa tacccacttt tagattatta aagaaagaac tctccaaaat ggagagttct
     1561 tgatcaagtc aagccgggga tgcttctact tcgtccttca ccggagaaaa ttggggatga
     1621 gacctgagcc gcaagtgcag cgcgtaatat ccaatcgctg ccaggatgca gatatatcct
     1681 ccgatctgcc agagtaaatt tgggttcgcg ttatcaagaa tatagccagc cgcgcctggt
     1741 ccaatcgcgg caggcaggga ccaggtgaga ccagagatgg ccatataacg cccgcgcata
     1801 tcctcaggag cgaaacctgc aaccagcgcc tggctggtgg gaaagaaaag catctcacca
     1861 atacagacga taatggcagc gactacgaac atcgcaaagc tggaaacgag tccatacatg
     1921 aagacaccga tcgcgaaaaa gatcgtactc aacatcatga tcaaaaatgg agcatgacga
     1981 cgaattttcc gactgatcca gaattgaaat agcacgactt ccagcccggt aatcgaaaga
     2041 atcaagccat acccttgact gtcgatgtga tgaacgtctc gcatgtaaac agaaagcgta
     2101 ctgtattgct gctggta
//
LOCUS       DASTJP010000272         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1287773, whole genome shotgun sequence.
ACCESSION   DASTJP010000272 DASTJP010000000
VERSION     DASTJP010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287773"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VFQ23_08200"
     CDS             <1..954
                     /locus_tag="VFQ23_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906608.1"
                     /translation="NAENALLLSSNNSMAHAVHSWALDFQEGKNGDAQESIINALEID
                     PNNALAHAYYAEILVDSGLFDNYAKAAEESRIAIALAPDLLEAHRARAYVLSVTGAEN
                     IEAAIQEYEAAIAINPNIAQLHIELGQSYRALQVYDEAITSFTRANTLNPTDYVPDLL
                     ISRTHATTGSYQQALQYAETAVKNAPENANLRGNYGVMFFRNFLYEEASEQLSLVVNG
                     GRTEEGTEITAIPLSDSPFVVEYYFTYGLALARTNQCGEALQIAQKVQTIVAEDDETA
                     LFAASEINRICEENLENPPAETPTPGSDDTATEESTPAAAP"
     gene            1050..2426
                     /locus_tag="VFQ23_08205"
     CDS             1050..2426
                     /locus_tag="VFQ23_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906609.1"
                     /translation="MLQQINRGEIEPLFLATPTPTRTTDSFILEGDANFTAGNLNAAI
                     TAYEEAVRVNPNDAETWRKLARIQTYSSAFLITNPEKKARLLDALESARKAVELAPED
                     STAHAVLAFVLDWNANSTLYDQREVQAYLVEAEQEALVALQLENTNTLALAYYTEILI
                     DQQKWNQAELQMQQALQNPDATQFMDVHRINAYLLETLGQYNLAITEYEKAITLEPNF
                     TFLYLRIGANYRRLAFEIQNPEAARPVYEKSLEYFDKAVDINTQIGVKDPAPHVSIAR
                     TYSQLGEFFAAARNIQKALEYEPQNADLYGQLGIVYFRSRNYEGSIYSFECAIRGCSG
                     EDSCLGRGLEKCFPDLGFNEVEVTGQAISPNTIVYYYTYGSVLAALSRQRDNKCPEAM
                     QIFDEVNAELNANPDDYADGRDTILSIVRAGVDICDSLASGIPIPTSGDESATSTPEI
                     EATSTPTP"
     gene            2590..2892
                     /gene="groES"
                     /locus_tag="VFQ23_08210"
     CDS             2590..2892
                     /gene="groES"
                     /locus_tag="VFQ23_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET9906610.1"
                     /translation="MASKLKLKPLGGRVIVEPIEQEEMTAGGIILPETAKEKPQEGKI
                     LAAGPGDRDEDGERIPMEVQVGDKVLYAKYAGTEVKVDGKKLLILKETDILAVVEG"
     gene            2909..>3985
                     /gene="groEL"
                     /locus_tag="VFQ23_08215"
     CDS             2909..>3985
                     /gene="groEL"
                     /locus_tag="VFQ23_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET9906611.1"
                     /translation="MAAKQLVFSEDARRKLRTGMNMVANAVGATLGPKGRNVAVDRKF
                     GSPTITHDGVSVAKEIELEDPFENMGAQLLKEAASKTNDIAGDGTTTSTVLAHAIVNE
                     GLKALEAGYNPMLLKHGIQQATESVVEELRKMSVKIDTREEIASVATNSAADESIGEL
                     IAGVMDKVGKDGVITVEESKSMADETEFVEGMQFDRGYISAYFITNADQMQAIISDAY
                     ILINEKKISAAQDIVPLLEKLVQIGKRELVIIAEDIDGEALATLVLNKLRGMLNVLAV
                     KAPGFGDRRKAMLQDIAALTGGTVISEETGRKLETATLQDLGRAEKVVADKENTTIVG
                     GKGKKGDIDARIKEIRAEIDKSTSD"
BASE COUNT         1063 a          980 c         1013 g          929 t
ORIGIN      
        1 aatgctgaga atgctttatt gttgagctca aataattcaa tggcacatgc tgttcactcg
       61 tgggccctgg attttcaaga gggaaaaaat ggagacgcac aggaatccat cataaatgct
      121 ttggaaatcg acccaaacaa tgcgctcgca cacgcttatt atgctgaaat actggttgat
      181 tctggtttgt ttgataatta cgccaaagct gcggaagagt cacgcattgc cattgcgctt
      241 gctcctgacc tgttggaagc gcatcgcgcc cgcgcctatg ttctttcggt tacgggggca
      301 gaaaacatcg aggcggcaat tcaggaatat gaagccgcca ttgcaatcaa tccaaatatt
      361 gcccaactgc acattgagct ggggcaaagc tatcgtgccc ttcaagtgta tgacgaagca
      421 attacctcat tcactcgtgc caatacatta aaccccaccg attatgttcc tgatctgttg
      481 atctcccgca cccatgccac cactggctcc tatcaacaag ctttgcagta tgctgagacc
      541 gctgtgaaaa acgctccgga gaatgcgaac ttgcgcggca attacggcgt gatgtttttc
      601 cgaaattttc tttatgaaga agcatcggaa cagctctcgc ttgtggtgaa tgggggcaga
      661 acggaagaag gcacagagat cactgcgatt ccgttgtcgg attctccctt cgttgtggaa
      721 tattatttca cgtatggtct ggcactggca cgcacaaatc agtgtggtga ggcattacag
      781 attgcccaga aggtacaaac cattgtcgct gaggatgatg aaactgcctt gtttgccgcc
      841 agtgagatca atcgcatttg cgaggaaaat ttggaaaacc cgccggcaga aacacccaca
      901 cctggctccg atgatactgc cactgaagaa agtacgcccg ctgccgcgcc atgaacgttt
      961 ccccgcgccg tcctcttttt agccgtaaac cacagtccaa cgtataccga atgttcctgt
     1021 gggtcgttat gcttttgggt ggcatatgga tgttacagca gatcaatcga ggtgagatcg
     1081 agcctttgtt tttagccacc ccaactccta cacgtacaac cgattcattt attctggaag
     1141 gggatgctaa ttttactgcg ggcaatctga atgcagctat taccgcctat gaggaagctg
     1201 tacgggtgaa cccgaacgat gccgagacct ggcgaaagct ggcgcgcatt caaacctatt
     1261 cctctgcatt cctgatcacc aaccccgaga aaaaagcgcg cttgctggat gcattggaat
     1321 ctgcaagaaa agccgttgaa ctagcccccg aagatagcac tgcccatgct gttctcgcct
     1381 tcgtgctgga ctggaacgca aactcaacgt tatatgacca gcgcgaggtt caggcgtatc
     1441 tagtggaagc ggagcaggaa gcgctggtgg cattacagtt agagaacaca aatactcttg
     1501 cacttgctta ttacacagaa atcctcatcg accagcaaaa atggaatcag gcggaattgc
     1561 agatgcaaca ggcacttcag aatccggatg cgacgcaatt catggatgta catcgcatca
     1621 atgcctattt attggaaacg ctcggtcaat ataacctggc gattactgag tatgaaaaag
     1681 cgattacact cgaaccgaac tttacattcc tatacctgcg catcggtgcg aattatcgcc
     1741 gcctcgcgtt tgaaatccaa aacccggaag cagcacgtcc cgtgtatgaa aaatcgctgg
     1801 aatactttga caaggcggta gatatcaata cgcagatcgg agtcaaggac cccgcgccac
     1861 acgtctccat tgcgcgtacc tattctcaac tcggtgagtt cttcgccgcc gcgcggaata
     1921 ttcaaaaggc tttggaatat gaaccgcaga atgccgatct ttatggacaa ttgggaattg
     1981 tttacttccg gtcacgtaac tatgaaggct ctatttactc ctttgaatgc gccatccgcg
     2041 gctgttcagg tgaagattcc tgtttgggac gaggcctgga gaagtgtttt ccagaccttg
     2101 gtttcaatga ggtggaagtc acaggtcagg cgatttcacc caacacgatc gtctattact
     2161 atacgtatgg atcagtgcta gccgccttga gccgccagcg cgataataaa tgccctgaag
     2221 ccatgcaaat ctttgacgag gtcaatgcag aactcaatgc caacccggat gactacgccg
     2281 atggccgcga tacgatcttg agtattgttc gggcaggggt ggatatttgt gattcgctgg
     2341 cctcgggtat acccattccg acctctggtg atgaatcggc tacttcgacg cctgaaatag
     2401 aagcaacctc cacaccgacg ccgtaatttc attttccgaa aggggatatc agaaatatat
     2461 aagaatccaa aaaatccctc ttgacttgcc attgtgagtt cgggtatcat tcattagcac
     2521 tctgacgtaa cgagtgctaa tttcatgcat tatgcaaaat ctgcaaaaat caaaacagga
     2581 ggtctgacaa tggcaagcaa attgaagctt aagccccttg gtggacgcgt gatcgtggaa
     2641 ccgatcgagc aggaagaaat gactgctggt ggaattatcc ttcccgaaac cgccaaagag
     2701 aaaccgcagg aaggcaaaat cctggcggcg ggcccaggcg accgcgatga ggacggtgaa
     2761 cgaatcccca tggaagtaca ggtcggtgat aaggttctgt acgcaaagta cgccggcacc
     2821 gaggtgaaag tggatggcaa gaaactgctc atcctgaagg aaaccgatat tttagccgtg
     2881 gtcgaaggct aattggagga ataacaaaat ggctgctaaa caacttgttt tttcagaaga
     2941 cgcacgccgc aagctcagaa ccggtatgaa tatggttgct aatgcggttg gcgcaactct
     3001 tggccccaag ggccgcaacg tggctgtgga ccgcaagttc ggttcaccga ccattacgca
     3061 cgatggcgtc tctgtcgcga aagagatcga acttgaagat cccttcgaga acatgggcgc
     3121 gcaattgctc aaggaagctg catccaagac caatgacatt gcgggtgatg gcactaccac
     3181 atccacagtg ctggcgcacg cgattgtgaa tgagggtctc aaagccctgg aagccggcta
     3241 taaccccatg ctcctcaagc atggcatcca gcaggcgacc gaatcggtcg tcgaagaact
     3301 ccgcaagatg tctgtcaaga tcgacactcg agaagagatc gcttctgtag cgacaaactc
     3361 cgctgccgat gaatcgatcg gtgaactgat tgcgggcgtg atggataaag ttggcaagga
     3421 tggagttatc accgtcgaag agtccaaatc catggcagac gagaccgagt ttgtcgaagg
     3481 tatgcagttc gaccgtggtt atatctctgc ctacttcatt accaatgcag accagatgca
     3541 ggccatcatt tctgatgctt acatcctgat caacgagaag aagatctctg ctgcacagga
     3601 tatcgttcct ttgctcgaga aactcgttca gattggcaag cgcgagctcg tcatcatcgc
     3661 ggaggatatt gatggtgagg cactcgccac cctggtgttg aataaactgc gcggtatgct
     3721 caacgtgttg gctgtcaaag ccccgggttt tggtgaccgc cgcaaggcaa tgttgcagga
     3781 tattgctgct ctcaccggtg gtaccgtgat ttctgaagaa actggtcgca agcttgagac
     3841 cgctacactt caggatctcg gtcgcgcaga gaaagttgtt gccgataaag agaacactac
     3901 gattgtgggt ggcaaaggca agaagggtga tatcgatgcc cgcatcaagg aaattcgcgc
     3961 agagatcgat aagagcacca gcgat
//
LOCUS       DASTJP010000273         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1387985, whole genome shotgun sequence.
ACCESSION   DASTJP010000273 DASTJP010000000
VERSION     DASTJP010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387985"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFQ23_08220"
     CDS             complement(<1..365)
                     /locus_tag="VFQ23_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HET9906612.1"
                     /translation="MSVLQKTSVIQDVIAMVEKKDAGQPEFHQAVREVMSSLEPTVEK
                     HPEFVRNKIYERIVVPDRAVTFRVSWVDDDGEVQVNRGYRVQFSNAIGPYKGGLRFHP
                     SVNLSIVKFLGFEQIFKNSL"
     gene            complement(512..823)
                     /locus_tag="VFQ23_08225"
     CDS             complement(512..823)
                     /locus_tag="VFQ23_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906613.1"
                     /translation="MNTPLVTPWADSAPPTESRLMQLCAEEGLSPYQWSNGPHDVYSA
                     HKHSYDKVIYVVRGSITFGLPEEGREILLKAGDRLDLPSNTVHDAVVGPQGVVCLEAH
                     G"
     gene            complement(820..1212)
                     /locus_tag="VFQ23_08230"
     CDS             complement(820..1212)
                     /locus_tag="VFQ23_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9906614.1"
                     /translation="MAPRPTNPIMKILSIDHVQIAMPPGEEEKARQFYINILGFTETP
                     KPAELVKRGGAWFHSENAHLHLGVEQGFTPARKAHPAFIVDELDLLITKAESAGYETD
                     TSQPPLDGYKRAHVFDPFGNRLELMQRL"
     gene            complement(1342..1707)
                     /locus_tag="VFQ23_08235"
     CDS             complement(1342..1707)
                     /locus_tag="VFQ23_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9906615.1"
                     /translation="MPNKRYSIFFAIVVLGLVTLACNALLPLPTEESTPTQIIEPGVT
                     QTQGNVPQSEAAVPRVSVEEAKAAFDSGAAVIVDVRSQTAYDIGHVAGSLFIPLGEFE
                     TNISNIDLPKDQWIITYCT"
     gene            complement(1770..3572)
                     /locus_tag="VFQ23_08240"
     CDS             complement(1770..3572)
                     /locus_tag="VFQ23_08240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET9906616.1"
                     /translation="MTISAPRWDLTNVYPSLESKEFQNAVKEYKSLLDKLEKFYKNKI
                     SKASSKTSVKELGSLLGKSVDQFNAIYELYGTIEAYIYSFITTNSRDKTAMRLYSEFE
                     QVQVRQSNLITQFRAWVGKLGDKLDKAIDSNPSAKAHGFTLRETRDQAKYMMSEAEEV
                     LATEMSLSGGNAFGKLQGTITSQISVDFQLNGKAEKLPMPALINLRTHADEDTRRRAY
                     ESENKAWEEVKETLAACLNGVKGETNTLYKKRGRKDALHSAIDAARIDRKTLDAMMGA
                     MNDSFPMFRKYFKAKAKKLGKEKLAWWDLFAPMGKTDKVYSYDEARDFILNYFGKFSP
                     DLHKMTETAFQNNWIDAEQREGKRGGAFCMAVPSVRESRILSNYDGSFDGISTLAHEL
                     GHAFHNDCAFKAGKTELQRDDPLTLAETASIMCETIITEAVLATTTDPQEELAVLEAQ
                     IQGAAQVIVDIYSRYLFEKEVLERREQAELSADDFNDIMERAQKATYGDGLDERYLQK
                     FMWTWKPHYYRSNFPFYNFPYAFGLLFGTGLFAIYQQRGDDFVKDYKDLLAATGEDTA
                     AKLARRFGIDITKRKFWEDSLAIIGKRVDRYSEL"
     gene            complement(3675..>4230)
                     /locus_tag="VFQ23_08245"
     CDS             complement(3675..>4230)
                     /locus_tag="VFQ23_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9906617.1"
                     /translation="GNLIIESGDITRKISERAAMTDLIVLKIVHPPMGGISTLRSPFR
                     TILLNSSRPLLGVPGKATPFKRAVLAFDGSDRSKEALFVATYLAEMWKTELIVFTGVD
                     SNKVKADTQDYVRRYLDVHEVEANYILSDGSVTDHLKKTAEERGADLLLMGSHGGSLM
                     QQLFAGSALDFILRESTIPIFICG"
BASE COUNT          993 a         1084 c         1018 g         1135 t
ORIGIN      
        1 agtgagttct tgaagatctg ctcaaaacca agaaatttga cgatgcttag gttaaccgac
       61 ggatgaaaac gcagcccgcc tttatagggt ccaattgcgt tgctgaactg cacccgatac
      121 ccgcggttca cctgcacttc accatcatca tccacccatg agactcggaa ggtaactgcc
      181 cggtctggta cgacaatacg ttcataaatt ttgtttctta caaattcagg gtgtttctcc
      241 accgttggct caagtgaact catcacctcc cgtaccgcct ggtggaattc aggctgacca
      301 gcatcttttt tctccaccat cgcaatgaca tcttgaatca cagacgtttt ttgaaggaca
      361 gacattttta tctcctttgt taaaacagga atcgtctgat gaaatgtaga ttgcaaaatt
      421 attttgaagg aaatgtcacc tcctcttttc tgacttctgc ctgatgccgt taaataaaac
      481 agggacgcat ttggcagcgc gtccctgttt cttaaccatg tgcctccaaa cagacgactc
      541 cctgtggacc tacaaccgcg tcatggaccg tgttcgaggg caggtcaagc cgatctcctg
      601 cttttagtaa gatctcacgc ccttcttcag gcaacccaaa tgtgatcgag ccgcgcacca
      661 cataaatcac cttatcgtag gaatgcttgt gcgcagagta cacgtcgtgt ggtccgttcg
      721 accattgata ggggctcaac ccctcctccg cgcagagttg catcagcctg gattccgtgg
      781 gaggagcgga atcggcccag ggcgtgacaa gaggtgtgtt catagtcttt gcatcaactc
      841 gagacggttt ccaaacggat cgaagacgtg cgcgcgtttg tatccatcca acggaggttg
      901 agacgtatcc gtttcatacc cggcactctc tgcctttgtg atcaacaaat cgagttcatc
      961 cacaataaat gccgggtgtg ccttacgggc gggagtaaaa ccctgttcga cgcccagatg
     1021 taagtgtgca ttttcagagt ggaaccatgc cccaccacgc tttacaagct cagccggctt
     1081 tggagtttca gtaaacccaa gtatatttat gtaaaactgg cgtgcctttt cttcttcacc
     1141 tggtggcatc gcaatttgaa cgtgatcaat ggaaaggatt ttcatgatgg ggttcgtggg
     1201 gcggggtgcc atcccgcctt acggctcaat cggatatcca gcagcttccc aggcatcaag
     1261 accgccaaga atagcgctgg cacgttcata accattctgc aaaagtagga acgccgcacg
     1321 ggcgctcgta tgttcgttcg gtcaggtgca gtaagtgatg atccattgat ctttagggag
     1381 atcgatattg gagatattcg tttcaaactc acccaacgga ataaatagcg aacctgcgac
     1441 atgacctata tcataggctg tctgactgcg tacatccacg atcacagccg caccgctgtc
     1501 aaatgcggct ttggcttcct ccaccgacac gcgcggaact gcggcttctg attgcgggac
     1561 gtttccctgc gtctgtgtca cgccgggttc tatgatttgt gtcggcgtac tttcttctgt
     1621 cggtaatggc agcagcgcat tgcacgccag cgtgaccagc cccagaacaa caatggcgaa
     1681 aaaaatgctg tatcttttgt ttggcatgtt cgctccttca agaatagttc agcccgcatg
     1741 aaatgcgggc tgaacgaaaa cttgagaact tacaattcgc tatatcgatc tacacgtttg
     1801 ccgataatgg caaggctgtc ttcccagaat ttgcgtttgg tgatatcgat accgaagcgc
     1861 ctggcaagtt ttgcagctgt atcttcccca gtcgcagcca ggaggtcctt gtaatctttt
     1921 acaaaatcat cgccgcgctg ctggtaaatg gcaaacaaac ctgttccgaa caggagcccg
     1981 aacgcatagg ggaagttata gaaggggaaa ttcgagcggt aataatgtgg tttccaggtc
     2041 cacatgaatt tctgaagata gcgttcatcc agcccatcgc catacgtggc tttctgcgcg
     2101 cgttccatga tgtcattgaa atcatctgca gaaagttctg cctgctcgcg gcgctccagc
     2161 acttctttct caaaaaggta acgtgagtag atatccacga tcacctgagc tgcaccctgg
     2221 atttgtgctt caagtacagc caattcctcc tgtggatctg ttgttgtggc aagcactgct
     2281 tccgtgataa ttgtttcaca cataatggag gcagtctcag ccagtgtcag gggatcgtct
     2341 cgttggagtt cagttttgcc agctttgaag gcacagtcgt tatggaaggc gtgacccagt
     2401 tcatgtgcca gggtgctgat gccatcaaac gagccgtcat agttagaaag aatgcggctt
     2461 tcccgaacgg acggtacagc catacagaat gcgccgccgc gttttccttc cctctgttct
     2521 gcatcaatcc aattgttttg gaaggcggtc tctgtcattt tatgcagatc aggtgaaaat
     2581 ttgccaaagt aattgaggat aaaatcgcgg gcttcgtcat aggaatagac cttatcggtt
     2641 ttgcccatcg gggcgaagag atcccaccag gcgagttttt ccttgcccag tttctttgcc
     2701 ttggctttga aatatttgcg gaacatgggg aacgaatcat tcatcgcgcc catcatggca
     2761 tccagtgtct ttcgatcgat acgtgccgcg tcgatcgcgg aatgtaaggc atcctttcga
     2821 ccgcgctttt tatagagggt attcgtctcg cctttgaccc cgttcaggca ggctgccaat
     2881 gtttccttga cttcttccca ggctttgttc tctgattcat aggcacggcg gcgcgtgtct
     2941 tcgtcagcat gagtccgcag gttgatcaac gcgggcatcg gcaacttttc cgctttgcca
     3001 ttcaactgga aatccacaga aatttgcgag gtgattgtcc cctgcagttt gccaaaggca
     3061 ttaccaccac tgagcgacat ttctgtggcg aggacttctt ctgcctcgct catcatatat
     3121 ttcgcttgat cgcgtgtttc tctgagcgtg aatccgtgtg cctttgcaga tggatttgag
     3181 tcaattgcct tatcgagctt gtcgcccagt ttcccaaccc aggcccggaa ctgtgtaatc
     3241 agattgctct ggcgtacttg tacctgctca aattcagaat acaagcgcat ggctgtcttg
     3301 tcacgtgagt tggtcgtgat gaaggaatag atataggctt cgatggtccc gtaaagctcg
     3361 taaatggcat tgaattggtc caccgatttg ccaagcaggg aaccgagttc tttcaccgat
     3421 gtttttgagc tggctttgct gatcttgttt ttgtaaaact tttcaagttt atccaaaagg
     3481 cttttatatt ccttcacagc attttggaat tctttcgatt caagcgaggg atagacgttt
     3541 gtgaggtccc aacgcggtgc cgagatagtc atgcagcaac tccttaagaa tgaagtgaat
     3601 taagtatatc aaattcccaa gatgcgctct tgatccccca tccgttgggc ctcacgccca
     3661 ggatctatcg attctcagcc gcaaataaaa atcgggatcg ttgattcccg taatatgaaa
     3721 tccagcgcac tcccggcaaa taactgttgc atcagcgatc cgccatgact gcccatcagc
     3781 agcagatcgg cgccacgctc ctcggccgtt ttcttcagat gatccgtgac gcttccatca
     3841 ctaagaatgt agttggcttc cacctcatgc acatcaagat aacggcggac ataatcctgc
     3901 gtgtcggctt ttaccttgtt gctatcgaca ccggtgaaga cgatcaactc ggttttccac
     3961 atctctgcca gatacgttgc cacaaagaga gcttccttcg agcgatcact tccatcaaaa
     4021 gccagcacag cccgtttgaa tggagtggct tttcctggaa cgcccagcag tgggcgagag
     4081 gagttcaaaa ggatcgtgcg aaatggagat cgaagagtgg atataccacc catcggtgga
     4141 tgaacgattt tcaagacgat caagtcggtc atggccgcgc gctcgctgat cttgcgcgtg
     4201 atatcaccag attcaatgat caggtttccg
//
LOCUS       DASTJP010000274        13221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1092018, whole genome shotgun sequence.
ACCESSION   DASTJP010000274 DASTJP010000000
VERSION     DASTJP010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13221
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092018"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFQ23_08250"
     CDS             complement(<1..217)
                     /locus_tag="VFQ23_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET9906618.1"
                     /translation="MTEYNSLEKIRLQKLEELRAEGIEPYPTRAHRTHTSAEAIAEFD
                     AAEKDGREVKATVAGRIRASRAMGKVSF"
     gene            complement(370..837)
                     /gene="greA"
                     /locus_tag="VFQ23_08255"
     CDS             complement(370..837)
                     /gene="greA"
                     /locus_tag="VFQ23_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET9906619.1"
                     /translation="MPNNFLTKEGYQKLQEELDYLRTAKRQEVANRLHEAMEGGELIE
                     NAEYEAAKNEQAFVEGRIQELDVLLATAKIIESNGKNKSDIVQIGSKATIKEGNFEPE
                     TFIIVGAAEANPRDGKISNESPIGKAILNHKAGDTVKVETPGGTYNVKILKIN"
     gene            complement(980..1714)
                     /locus_tag="VFQ23_08260"
     CDS             complement(980..1714)
                     /locus_tag="VFQ23_08260"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET9906620.1"
                     /translation="MRWIYLSPHLDDAVLSAGGLIYDQTQAGDSVEIWTFMCGYPPEG
                     EFSPIADQLHQQWGFSSAEEAVRKRRLEDKNAAAILGATTRHFDFLDCVYRQGSNGEW
                     LYSKITVPPHKEDEKLPAQIAEAISARLHPDDVLVCQLAVGSHVDHVLVRRAAELLGK
                     SLRYDIDVPYIFYKPQELKPASAGMMDLVYSVSKAGVEFWKEAVLEYKSQISTLGKVF
                     DTPEKVRESIQNYWSDHQGIRLFEKG"
     gene            1831..2205
                     /locus_tag="VFQ23_08265"
     CDS             1831..2205
                     /locus_tag="VFQ23_08265"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9906621.1"
                     /translation="MEERRKQQRKDLMSYSQVFDLYNGKLIGYLGDLTQMGAMVIAEE
                     PMEIDTTLTISIQLPDLPKIKATRVALPVRVAYCHKDVSPEYFNIGLQFKLVTDHQKT
                     VIEAVIKNYEFKRSSSESHTQA"
     gene            2397..3872
                     /locus_tag="VFQ23_08270"
     CDS             2397..3872
                     /locus_tag="VFQ23_08270"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9906622.1"
                     /translation="MSTASGRHFFISYSRTDTNQKQNIVKQLRARGINLWVDIEKLVP
                     GTPTWEREIERAIRSAAGLIVLLSPESNNSEWVRREVSFAEQQDKVVFPVLIRGDEDD
                     SIPLRLSNHQWVDLRGNFEEGLDQLADALSDHLGITVVNKELRQQTKEPFKLKPEDLR
                     KFALPGLFALIGLIFLGCLVIVGGFIRSALSPQSTQAVTLTSPATDPAVTAINTDVVI
                     PDGLSGKIVYTCQIDKANSSDQICIINADGTGQLQLTDSNDNQDASFAPDGKSVVFVS
                     NQTGNYEIVEMDLSGNRTQLTDFKSSLGLPAISPDDQWIAFTNRVDGFEQLWLMDRDG
                     SNARPIFSLPGIAAVAPSWSPDSQELLFAVGKDLARQLFITGLDGAEPRLLSDTIFTP
                     GRTDWSRQGQIAYFIGEAWKREIWTIYPDGTGMAQVTNGGNAQSPSFSPGGRYIAYTA
                     YTNVDGRDEFSCEIFIMDLYSRESRQLTDNEYCDYQPRWGN"
     gene            4043..4513
                     /locus_tag="VFQ23_08275"
     CDS             4043..4513
                     /locus_tag="VFQ23_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906623.1"
                     /translation="MRKYRPVIAAVTALLFYTIADILVWQRIFEANNMVEHADTYHIG
                     WFVSLAGYATIGLLLMWGEWKDCFFFLISLFVGAFSGLEDVLYYLLDRKPMPESLPWL
                     DGNPMIYHVSREGVLLSVLFWLAGLVVLYIWLYWRRNDRHQLPQMSFEGEGTTS"
     gene            4879..6375
                     /locus_tag="VFQ23_08280"
     CDS             4879..6375
                     /locus_tag="VFQ23_08280"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HET9906624.1"
                     /translation="MQILIDSIGILYLLVIVGLSLYGLNSFLTAILYLLSNSQAKKRK
                     KLPILKEWPAVTIQLPIFNEKYTVERLLRAVTELEYPSERLQIQVLDDSTDDTAHLVK
                     NLVEEYKLDGLNIEWINRIDRKGYKAGALSEALKSATGELIAIFDADFVPRSDWLKKT
                     VPLFQNRKLGCLQTRWGHTNRTYNSLTRAEALAIDGHFIIEQTARSKHDLFLNFNGTA
                     GIWRRACIEDAGGWQWDTLTEDLDLSYRAQMRGWKIDYLPDVVVPAELPAQVEAFKKQ
                     QFRWAMGSSQVVRKILPKVFESGLPWRIRLMAVLHLTGYFVHPLMLASLLLTLPAGLL
                     FPKGFTFFPISIIAGLGPPLLYLLARAPDSPPLHERLTLLPLLTLTGFGISLNTSIAV
                     VQGLVKKGGAFIRTPKLNLGNRSKQRKDIENRYLPPISAVVWAEIGLGIYAFITGIVL
                     QDSVGWGVVPWMILYTLGYFYIAGLNLLQHTPVRSARPTKSLTAHSAD"
     gene            6602..8296
                     /locus_tag="VFQ23_08285"
     CDS             6602..8296
                     /locus_tag="VFQ23_08285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9906625.1"
                     /translation="MEDLTGKQFGHYQIVAPLGEGGMAAVYKAYQPNMERYVAVKVLP
                     RHMAASEEFTARFRREARMLAQLQHPHILPVFDYGEADGYPYIVMPHIVSGTLADVLR
                     SKRLSLSEVRRILTQLGDALSYAHARGMIHRDIKPSNVLVDERGNCLLTDFGLARMAE
                     ASTNITTSGAIMGTPAYMSPEQGAGSTVDHRSDIYSLGIVLYEMVTGRVPYIAETPVA
                     VVFKHIQDPLPSIHKFNPDLPDSVELVLRKVLAKSPDDRYQNAEDFVSALQNAISDTA
                     PKSTAHPDISTIPIEPVPPKELHPARNWILPIGFLVAGLALGALWINTRFNSQGPSIQ
                     PSSTALQSPTSIPAATMTQAIETTPLPSATIVAPPSDKTFPYLNDIQLLEIDSFDEPL
                     ASGWTVDVGIIENGLMEIIGNENWYGASYNSGIGEFQAAVIDFIYPTDTSLFEMYLEN
                     GAWNTAQYKRVGLYVGSNQADTNIYIGTSDHGGTKLSGNLALKPETTYSLFIAVLPNN
                     EFLEVIWNPADPSETLSHREKIDENWDGITWTLWIGGYQTTIQFDNFQKIAFSSVK"
     gene            8415..8780
                     /locus_tag="VFQ23_08290"
     CDS             8415..8780
                     /locus_tag="VFQ23_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906626.1"
                     /translation="MDSSDSLQFNIEVSTDDASEDEIDRLTRHLLLELRELDVESAKL
                     AKGGPAPAGSKGDTITMGAIVLEVLPAVLPSVLGLLQSWMSRGRGRHVKFKGMGIEFE
                     GSRDDLEKILEMLSKGGQQ"
     gene            8777..10954
                     /locus_tag="VFQ23_08295"
     CDS             8777..10954
                     /locus_tag="VFQ23_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9906627.1"
                     /translation="MSRKFALIIANTVYEDPGLAQLTAPGKDAKEFGRVLDSREICAF
                     DDVMILFNEDVFKVNESIDYFFSEKKPDDLLVLYFSGHGVRDEFGSLYLAVKNTNRNR
                     LRSTAVKSEFITEVMDQCRSRRQVLILDCCNSGAFAQGTKAATGVSIGTASAFEGKGY
                     GRVVLTASDSTQFAWEGDQVIGTTENSLFTHYLVKGLEGEADSNGDGRVTIDELYDYA
                     YEQIVNVTPKQTPGKWSYKQQGEITLRHNIKPEDTRPVPLPADLISAINSSLPSVRQA
                     AVGQLEELLKGKHVGRARSARLVLEKLAADDDSRHVAQAASRALDGIPQEVQNAETEQ
                     PVDLTRTAKLRRDQLEKEKQFRAEQTRLMEENAELEKRLDEERSTREKMQAVRTEPAA
                     TKMRWSPLTIGAVILTIAIIGFGLTRMVPIFTPEPTPEPTRTIPPTVEATASSTKAAV
                     VPPVATEETRTPTEVPSLTPAPTETLVPVPSGPLPELTDEKGGAMVFVPEGRFVMGTT
                     RGDLDERPVHSVYLDGYYIDKFEVTNALYEPCVRAGVCKAPIKADSFTRSSYYGNPAY
                     DNYPVIYVDWNMANAFCEWRGASLPTEAQWEKAARGTDERVYPWDKGLDCQKANFTFN
                     SRVCIGGTSKVDNYESGKSPYGVYDMAGNVWEWVADWYSETYYTSPSSTNPPGPDSGR
                     ARVARGGSWTRSDGEIRSANRINYAPTYFNFDLGFRCAKPISP"
     gene            11030..11890
                     /locus_tag="VFQ23_08300"
     CDS             11030..11890
                     /locus_tag="VFQ23_08300"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9906628.1"
                     /translation="MESSEPIQFSIEVSDMNAAVEDIDEMTRQLLSELREMNVESAEL
                     TKGGPAPRGSKGDFVTLGSIAVSVLPAVLPNLISLVRDWAGRGHGRTVKFKGMGIEFE
                     GSPEEFQKLMASLDAGGKVADEGKRARSPKIFISYRRSDSADVTGRIYDRLVEQFGKS
                     AIFKDVHSIPPGIDFREHLGKAVGKCKIFLVIIGDQWLEVTDAKGKKRLKDPDDFVRI
                     EIESALQRDIPIIPLLVRGATMPAEAKLPRTLRELAFRNGIPVRADPDFHRDMDRLIE
                     AVSGYSSKKS"
     gene            11924..>13221
                     /locus_tag="VFQ23_08305"
     CDS             11924..>13221
                     /locus_tag="VFQ23_08305"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HET9906629.1"
                     /translation="MSGKYALIIGNTEYIDSGLAQLTAPGKDAEDFARVLKDKEISAF
                     DDVKVIVNEPSALVIESIDEFFDQKKPDDLLVLYFSGHGIRDEIGSLYLAFKNTIRTR
                     LRATAIKADYIRESMDQSRSKRQVLILDCCNSGAFPPGTKAELGGAMGITRAFQGYGR
                     FVLTASDATQFAWEGDQVIGTQTDNSLFTHFLVKGLEGEADNDGDGQITVDEIYDYAF
                     QKISQITPNQTPTKSASKQAGEIVLRENMRFEDIKPVALPDDLISEIEDLRPSVRESA
                     VRWLETIVKGKNIGMARSAIDALEKVASDENTTRRVSEMAMRVIESVRAAEKKAEEER
                     KAREEAERRAALEAEEKRLTFEKAEAERKAKEEAEKLARAKAEREAAEQEAARLKAER
                     EEAEKAARDATEKASKEKAEKEALDRETALKAERDAMDFA"
BASE COUNT         3488 a         3127 c         3321 g         3285 t
ORIGIN      
        1 caaatgaaac cttccccata gcccgggagg cgcggatgcg gcccgccaca gttgctttga
       61 cttccctgcc atccttctcg gcggcatcga attcggcaat tgcttcggcg ctggtatgcg
      121 ttcgatgtgc gcgcgtagga tacggttcga tcccttcagc acgcaattct tctaatttct
      181 gcaaacggat tttttcgagt gagttgtatt ctgtcatgtc catgctccca acccccacca
      241 ccgccctccc caaaaattga cgaacctcag tcgaatttag gagagggggc cggaggcggg
      301 agggggtata aataaacccc gcgtcctgtg caggcacgcg gggtgaaacg ttacgctcct
      361 gcgtattgct caattaatct tcaggatttt aacattatat gtgccacccg gggtttccac
      421 ttttacagtg tcgcctgctt tgtgattgag gatggctttg ccgatgggcg actcattgga
      481 aatcttgcca tcacgggggt tggcttctgc tgcaccgaca atgataaagg tttccggttc
      541 aaaattacct tccttgatgg ttgcctttga accgatttga acgatatcac ttttattttt
      601 tccgttactt tcgatgatct ttgcagttgc cagtaaaacg tcaagttcct gaatgcggcc
      661 ctccacaaag gcctgttcat ttttcgcggc ttcatactcg gcattttcga tcaattcccc
      721 accttccatg gcttcatgca aacggttggc aacttcctgc cgttttgcag tgcgcagata
      781 gtccagctct tcctgaagct tctggtaacc ttctttggtc aaaaaattgt tgggcatgtc
      841 ttttttccta ctataagaat attgcatctc taaaaagaga tgcaaatggg taagacctgg
      901 ttaaatcccg tcaggcgcgc ttaatatatc acgattttat ggtcaataca agagactgga
      961 ggggagaatc cctgccgaat taaccttttt cgaacaaacg gatgccttga tggtcagacc
     1021 agtaattttg aatggattca cggacttttt ccggggtatc gaacaccttg cctaaggtgg
     1081 aaatttggga tttgtactct aaaactgcct ccttccaaaa ctctacacct gctttactaa
     1141 ccgaatacac caaatccatc atcccagccg aagccggctt caattcttga ggtttgtaga
     1201 aaatataggg aacatcaata tcataacgga gcgattttcc taaaagttcc gcagcccgcc
     1261 gcacaagaac atggtccacg tgcgaaccga cggctagctg gcagaccagc acgtcgtccg
     1321 gatggagccg ggcagaaatg gcttccgcaa tttgtgcagg aagcttctca tcttccttgt
     1381 gcggaggcac agtaatcttg gagtagagcc actccccgtt ggatccttgt cgatagacac
     1441 aatccaggaa gtcaaaatga cgggtagttg cgccaagaat ggcagctgcg tttttgtcct
     1501 caaggcgccg cttgcggacc gcctcttccg cagaggaaaa tccccattgc tgatgaagct
     1561 ggtcagcgat gggtgaaaat tcgccttccg gtggatatcc gcacatgaac gtccagattt
     1621 caaccgagtc acccgcctgg gtctgatcat agatcaagcc accagccgaa agcaccgcgt
     1681 catcgagatg aggggaaagg tagatccagc gcatagcatc gatttaacca tagtttgtcg
     1741 cgcggacagt aagattttaa gatggcagtg caacgaatca aggtcatcat ttctagtaca
     1801 ctttatagac ttttctggag gcttaactag atggaagaac gtcgtaagca gcaacgcaag
     1861 gatttaatgt cctattcaca ggtatttgac ctgtataacg gcaaattgat cggttacctg
     1921 ggtgacctca cccaaatggg agcgatggtt atcgcagagg agccgatgga aattgataca
     1981 actttgacaa tttcaataca actcccggac cttccaaaaa tcaaggcgac tcgtgtcgct
     2041 ctgcctgtac gcgttgctta ttgtcacaaa gacgttagcc ctgagtattt caatattgga
     2101 ctccaattta aactggtgac agatcaccaa aagacggtga tcgaggcggt gataaaaaac
     2161 tacgagttta aacggtcctc ctcggaatct cacacgcaag cgtagcgatc tcttacagaa
     2221 ggaaaagaga cccaggcata gaaagccaga aggtctcttt ttttgttttc ctgcaaagtg
     2281 ccgccctaaa ttcaggattt gctcatcact taatagaaaa tttccggcaa acagcaagat
     2341 agcggtaaac ctgtttttac tctataatgc cgggatacca aaacagagag tctttcatga
     2401 gcactgcctc tggccgccac ttcttcatta gttacagccg cacagataca aaccaaaaac
     2461 agaatattgt caaacaactt cgcgcccgcg gcatcaattt gtgggtggat atcgagaagt
     2521 tggtgccagg aacgcccacc tgggagcggg aaatcgagcg cgccatacgc agcgcggcag
     2581 gtcttatcgt cctgctttcg cctgagtcta ataactcgga gtgggtgcgc agagaagtca
     2641 gttttgccga gcaacaagat aaggttgtgt ttcccgtttt gattcgcggc gatgaggatg
     2701 attccatccc gctgaggctt tccaaccatc agtgggtgga tttgcgagga aactttgaag
     2761 agggactgga tcaactcgcc gatgccctga gtgatcatct tggaattacg gttgttaaca
     2821 aagagctacg acagcaaacg aaagaacctt tcaaactcaa accggaagat ctgaggaaat
     2881 tcgcgctgcc cgggcttttc gctttgatcg gcctgatctt tcttggatgc ctggtgattg
     2941 taggaggttt tatccgaagc gctctttcgc ctcagtccac acaggcagtg acacttactt
     3001 cgccggcaac ggacccggcc gtaacagcaa tcaatacaga cgtcgtcatc cctgatggac
     3061 tgtctggcaa gatcgtttat acctgccaga ttgacaaagc aaactcctcc gatcagatct
     3121 gtatcatcaa tgccgacgga acgggtcagc tccagctaac cgactcaaac gataatcagg
     3181 atgcctcttt cgcccctgac gggaaatcgg tcgtcttcgt cagtaaccaa accgggaatt
     3241 atgagatcgt ggaaatggac ttgtcgggaa atcgaaccca actcactgac ttcaaaagct
     3301 cacttgggtt acctgcgatt tccccggatg atcagtggat tgcattcacg aatcgagtag
     3361 atggatttga acaactctgg ttaatggatc gagacgggag caacgcacgg ccgatcttta
     3421 gcctgccggg tattgcggcg gtggccccca gctggtcacc agacagccag gagctattgt
     3481 tcgcagttgg caaagatctg gcccggcaac ttttcattac gggactggac ggcgcggagc
     3541 cgcgtctgtt gagcgatacg attttcacac ctggacgaac agactggtca agacaagggc
     3601 aaattgctta cttcattggc gaagcctgga agcgcgagat ctggacgatt tatccggacg
     3661 ggacaggcat ggcgcaggtc acaaacggtg gaaacgcgca aagtcccagc ttttctcccg
     3721 gtgggcgtta cattgcttat accgcctaca ccaatgtaga tgggcgcgat gaatttagct
     3781 gcgagatctt catcatggac ttatattcac gtgaaagccg gcagctcacg gataacgaat
     3841 attgcgatta tcagccaaga tggggaaact agaagtgtat cttcgagtaa gttgaacgta
     3901 tagaaaacgt acggaaaata taagctgttt gcaaggtgat cgcttgtatc tcctgtggat
     3961 actcccacgt cgtataaagg aggctgcacg tagcaattgt aggtgcggct aaaactttag
     4021 aatctagtgg agtgccctgt acatgagaaa atatcgcccc gtcatcgccg ctgtaactgc
     4081 ccttctgttc tacacaatcg cagatatcct cgtctggcaa cggatttttg aagccaacaa
     4141 tatggtagaa cacgccgata cgtatcatat cggctggttt gtttcgctgg cgggctatgc
     4201 gaccatcggg ctccttttaa tgtggggtga atggaaggat tgcttctttt tccttatttc
     4261 cctgtttgta ggtgccttca gcggactgga agatgtgctg tactaccttc tggatcgcaa
     4321 acccatgccc gagtcactgc cctggctgga cggcaaccca atgatctatc acgtctcacg
     4381 agaaggggta ctgctctctg tgcttttctg gctggctgga ctggtagtac tctatatctg
     4441 gctttattgg cgtcgcaatg accggcatca actgccccaa atgagcttcg agggcgaagg
     4501 taccacttca tagtctcact tgcacgtttc atacaatcgc aggatctaag atcctgcgat
     4561 ttttttaatg tctgcagtta tttccaacgt caattctttg gggatttagt agatttctaa
     4621 aattcaatca ggcattcctt cttatcgtca atgagatggg tcaaattgac ccatctgtac
     4681 tattaattca tttttagtca gaatctcagc ggcatttaat tcaggactaa ggcatcattc
     4741 acccaacggg tgatattttt ttgcttcttt cgttctgata tgtatagcga aagtacgatg
     4801 gtcatttcga tttggaatgt tacgccgaag ggctgaccaa aggttttccg cattctaata
     4861 attaaggaga agagaacgat gcaaatactg atcgatagta ttgggattct ctacttgctc
     4921 gtcatcgttg ggttatctct ctacggactt aacagtttcc tcacagcgat tttgtacctg
     4981 cttagtaatt cgcaagcgaa gaaaaggaaa aaactaccca ttcttaaaga atggccagcg
     5041 gtgaccatcc aattgccaat cttcaacgaa aaatatacag ttgaaagatt attgcgcgca
     5101 gttaccgagt tggaataccc gtccgaacgg cttcaaatcc aggtgctgga tgactcgacg
     5161 gatgataccg ctcatcttgt taaaaatctg gtcgaggaat ataagctcga cggcctgaat
     5221 atcgaatgga tcaatcggat cgaccggaaa ggatataaag cgggtgccct tagtgaggcg
     5281 ctgaaaagtg cgaccggtga actgatcgcc atttttgacg cggacttcgt ccccagatca
     5341 gactggctga aaaagaccgt tcctctattc cagaatcgaa agctgggatg tttgcaaact
     5401 cgctggggac ataccaaccg tacctacaat tccctgacac gcgccgaggc attggcaatc
     5461 gatgggcatt tcatcattga acaaacggcg cgttcgaaac acgatctctt cctcaatttc
     5521 aacggcactg ctggaatttg gcgccgtgcc tgcatcgaag atgcgggtgg ctggcagtgg
     5581 gatacgctga ccgaggatct cgacctgagc taccgcgctc agatgcgcgg ctggaaaatt
     5641 gactatcttc cggacgtggt ggtgcccgca gaattaccgg ctcaggtgga agcctttaag
     5701 aaacaacaat tccgctgggc aatgggaagt tcacaggtag ttcgaaagat attgccaaaa
     5761 gtcttcgagt ctggcttacc ctggaggatc cgtctgatgg ccgtcctgca tcttacaggg
     5821 tactttgtgc atcctttgat gcttgcttcc ctattgctga cgcttccagc tggcttacta
     5881 tttccaaagg gctttacatt ttttcccata tccatcatcg cgggtttggg acctccattg
     5941 ctttacttat tggccagagc tccagacagt cccccactgc atgagagatt aacgctgctt
     6001 ccattattaa cactcaccgg gtttggcatc tcgctaaaca catcgattgc cgtagtacag
     6061 ggcttagtca aaaaaggtgg cgcattcatc cgcaccccga aactgaatct cggaaatcgc
     6121 agtaaacaac gcaaagatat cgagaatagg tatctgcctc ccatcagcgc cgtggtatgg
     6181 gcggagattg ggcttggcat ttatgcattc atcacgggca ttgtgctgca ggactccgtt
     6241 ggttggggag tcgtcccatg gatgatcctt tatacactcg gctacttcta catcgccggg
     6301 ttgaacctcc tccagcatac accggtcagg agcgcaagac ccacgaaatc cttaacggct
     6361 cacagcgcag attaagtctg agaaaaatcg tttcaaataa gccgtccgag ctttaaaacg
     6421 ctcggacgtt ttattttcag atcgtgtcag atattcttaa ttgcatttcc atacaaccat
     6481 gagataattc gtaagcgagt agttataatt cggtagtcct caacaaagat ggaaaagtaa
     6541 tttgatcgat cgtgttccaa agaccagtgt gatcagggtg tggtagttaa gcaggtaaat
     6601 tatggaagat ctgactggaa agcaattcgg acattatcaa atcgtcgctc cgcttggcga
     6661 aggaggtatg gccgccgttt ataaggccta ccagccaaat atggaacggt atgttgccgt
     6721 taaggtacta ccgcgtcata tggcggcctc tgaagagttc accgcgcgat ttaggcgcga
     6781 agcccgcatg ctggcgcaac ttcagcatcc acatattttg cctgtatttg attacggtga
     6841 agcggatggt tatccctaca ttgtcatgcc gcacattgtc agtggaactt tggcagacgt
     6901 tctgcgtagc aaacgcctgt cactttcaga ggtgcgacgt atcctaactc agctcggtga
     6961 tgcactaagt tatgcccatg cccgcggcat gattcatcga gacatcaaac ccagtaatgt
     7021 actggtcgac gagcgtggca attgtctgct gacggacttt ggtttggctc gtatggcaga
     7081 ggcatccaca aacattacaa cgtcgggagc gatcatgggc acaccagcct atatgtcgcc
     7141 agaacaaggg gcaggatcta ccgtcgacca tcgcagtgat atctactcgc ttggcattgt
     7201 tttgtatgaa atggtcactg gacgagttcc gtatattgca gagacgcctg tcgcggtggt
     7261 ctttaagcat attcaggatc cccttccctc cattcacaaa ttcaatcctg atctacctga
     7321 ctcggttgaa ctagtccttc gtaaagtgct tgcaaaaagc cccgacgacc gttatcagaa
     7381 cgcagaagat tttgtgagcg ctctacagaa tgcgatttct gataccgcgc cgaaaagcac
     7441 ggcacaccca gacatctcta cgattccaat tgagccggtt cctcccaaag agctacatcc
     7501 agccagaaac tggattctgc ccatcggatt tctggttgca ggattggcac ttggcgcatt
     7561 atggataaat acgcggttca attcgcaggg accttccata caaccaagtt ctaccgctct
     7621 tcaatctccg acatccatac cggcagccac gatgacgcaa gcaatagaaa ctacaccatt
     7681 gccttcagca acaatcgtgg ctcctccttc agataaaact tttccatatt tgaatgatat
     7741 acagctcctg gaaatcgaca gctttgatga gccgcttgcg agtggctgga ccgtggacgt
     7801 ggggattatt gaaaacggac tgatggaaat cattgggaac gagaactggt atggggcttc
     7861 ttacaatagt ggcattggag aatttcaggc agctgttata gactttatat atccaaccga
     7921 tacctctctt tttgagatgt acttagaaaa cggagcctgg aatacagccc aatacaaacg
     7981 tgttggactt tatgtcggca gcaatcaggc cgatacgaat atatacatcg gtacgagcga
     8041 tcatggaggc acgaagttat ccggaaattt ggcgctcaag cctgaaacca cttactcgtt
     8101 attcatagcc gttcttccca ataatgaatt tctagaggtc atttggaatc cggctgatcc
     8161 ctcggaaaca ctttctcacc gcgagaagat tgatgaaaac tgggatggaa tcacctggac
     8221 cctatggatt gggggctacc aaactacgat ccaatttgat aatttccaaa aaatagcgtt
     8281 tagcagcgtt aagtgaattg aaattaactg gtgctcgcct gtttacaggc ttataatggg
     8341 gctattggcg gaccaatagc ttcaaagtag tttcctttaa cgatccaatg ggttaataag
     8401 agaggattgc ccgcatggat agttccgatt ctttgcaatt caacatcgaa gtttcgaccg
     8461 acgatgcatc agaagatgag atcgatcgtc tcacgcgtca cctgctcctc gaactgcgag
     8521 agctggatgt tgaatcggca aaattggcaa agggcggacc cgcaccagca ggttccaaag
     8581 gcgataccat aacaatgggt gcaatcgttc tggaagttct tcccgcggtc ctgcccagcg
     8641 tgctgggcct gctacaatcc tggatgtctc ggggacgcgg ccgtcatgtc aaatttaagg
     8701 ggatgggaat cgagtttgaa ggctcgcgtg atgatcttga aaagatcctg gaaatgcttt
     8761 caaagggagg gcaacaatga gccgaaaatt cgcgctgatc attgccaaca cagtatatga
     8821 ggatcccgga cttgctcaat taactgctcc aggcaaggac gcaaaggagt ttgggcgggt
     8881 actcgattcg cgtgaaatct gcgcttttga tgatgtcatg atccttttca atgaagatgt
     8941 tttcaaggtc aatgaatcga ttgattactt cttctctgaa aagaaaccgg atgatctgct
     9001 ggtcctatat ttttcagggc atggcgttcg cgatgaattc ggctctttgt atctggcagt
     9061 gaagaatacc aatcgcaatc gcttgcgctc gacggccgtg aaatctgaat tcattacaga
     9121 agtcatggat cagtgtcgtt ccagaaggca agtgctcatt ctggattgtt gtaacagcgg
     9181 cgcatttgcg cagggaacga aagctgcaac cggggtaagt attgggaccg cctcggcttt
     9241 tgaaggcaag ggatatgggc gcgttgtctt aaccgcgagc gactcaaccc agttcgcctg
     9301 ggagggcgat caggtaatcg gcacaactga aaattctctg tttacacatt atctggtcaa
     9361 agggctggag ggtgaagcag atagcaacgg cgatgggcgc gtcaccatcg atgaattgta
     9421 tgattatgcc tatgaacaga ttgtaaacgt caccccaaag cagacgccgg gcaaatggtc
     9481 ttacaagcaa caaggtgaga tcaccttacg gcataacatt aagcctgaag acaccagacc
     9541 agttccatta ccagccgatc taatttcagc cattaatagt tcgctgccat cagtgcgtca
     9601 ggcggctgtc ggtcaattag aggaattact gaaaggtaaa cacgtcggac gggcgcgttc
     9661 agctcggctt gttcttgaga agctcgcggc ggatgatgat agtcgacatg tggcgcaggc
     9721 cgcctcacga gcgttggatg gcattccgca agaagtacag aatgcggaaa ctgaacagcc
     9781 agtcgatctc acccgtaccg cgaaactacg ccgtgaccaa ctggagaagg aaaaacaatt
     9841 ccgggccgag caaaccagat tgatggagga aaatgccgaa ctcgaaaagc gcctggatga
     9901 agaacgaagt acccgcgaaa aaatgcaggc cgtccgaacc gagcctgctg ccacgaaaat
     9961 gcgctggagc ccattaacga taggtgctgt catcctaaca attgcaatca ttgggtttgg
    10021 cttgacgaga atggttccca ttttcacacc tgagccgacg cctgaaccaa ctcgcaccat
    10081 cccaccgacg gtagaggcaa cagcctcttc aacgaaggca gcagttgttc cccctgtagc
    10141 gaccgaggaa acacgaacgc cgacggaagt gcctagcttg actccagccc ctacagaaac
    10201 tttggttcca gtaccgagcg gtcctttacc tgaactaaca gacgagaagg gtggagcaat
    10261 ggtatttgtg ccggaaggga gattcgtcat gggcaccacc cgtggtgatt tagatgaacg
    10321 accggtccat tcggtgtacc tggatggtta ctatattgac aaatttgaag tgaccaacgc
    10381 gctctatgaa ccgtgtgtga gggctggcgt atgtaaagca ccgataaaag cagactcatt
    10441 cacacggagc agttactacg ggaatccggc ctatgacaat tacccggtca tttatgtaga
    10501 ctggaatatg gcaaatgcct tttgcgagtg gcgcggcgcc agcctcccaa ccgaagccca
    10561 gtgggaaaaa gccgcgcggg gcaccgatga aagagtgtat ccgtgggata aagggttaga
    10621 ttgccaaaaa gcgaatttca cttttaactc ccgagtttgt attggaggga ccagcaaagt
    10681 tgataattat gagagcggga agagtcctta tggcgtctac gatatggcag ggaatgtttg
    10741 ggaatgggta gctgattggt attctgaaac gtattacacg agtccatctt cgaccaatcc
    10801 cccgggtcca gattctggac gtgcccgcgt cgcacgcgga ggctcctgga ctcgcagcga
    10861 tggcgagatt cgttctgcca accgcattaa ttacgcgccc acgtatttca actttgacct
    10921 tggttttcgc tgtgccaaac caatttcacc ctaattgatt tagttgagtc tctgatcaag
    10981 agcctttcca tcaattatta gtttagaagt ccattcgagg attccggtta tggaaagttc
    11041 tgagcctatt cagtttagta ttgaagtttc tgacatgaac gcagcggtcg aggatattga
    11101 tgagatgaca cgtcagctgc tttcggagct gcgtgagatg aatgttgaat ctgcggaact
    11161 taccaaaggc ggccccgcac cccgaggttc aaaaggtgat tttgtaacat tgggatccat
    11221 cgctgtttct gttttgcctg cagttctgcc aaatctcatc agcctcgtgc gggattgggc
    11281 tgggcgtggg catgggcgta cggtgaagtt caaggggatg ggaattgagt tcgaaggatc
    11341 accagaagag tttcaaaaac tgatggcttc actggatgct ggaggaaagg tcgcggacga
    11401 aggcaaacgg gctcgatctc ccaaaatttt tatttcatac agaaggtcag atagcgcaga
    11461 tgtcacggga cggatctatg accgactcgt ggagcaattc ggtaaatctg ccatcttcaa
    11521 ggatgtgcat tcgattccgc cggggatcga ttttagagaa catttgggaa aagctgtcgg
    11581 caagtgcaaa attttcctgg tcatcattgg ggatcaatgg ctggaagtaa cagacgcgaa
    11641 gggaaaaaag cggctaaaag atcccgatga tttcgtgaga attgagattg agtctgcact
    11701 ccagcgagat attcccatca ttccactttt agttcgcggg gcaacaatgc ccgcagaagc
    11761 gaagttaccg cgcaccttgc gagaactggc gtttcgaaac gggatccccg ttcgtgccga
    11821 ccctgatttc catcgggata tggatcgatt gattgaggcg gtatcgggct attcgagtaa
    11881 gaaatcgtag cttgtaacag atactgaaga gtagagagag aaaatgagcg gaaaatatgc
    11941 tctgatcatt ggcaacaccg aatacataga ttctggtttg gcacaactca ccgcgccagg
    12001 caaggatgca gaagatttcg cgcgcgttct caaagataaa gaaataagtg cttttgatga
    12061 tgttaaagtt atcgtaaacg agccgtctgc tttagtaata gagtcaattg atgaattttt
    12121 cgatcagaag aagccagatg atctccttgt cctttatttt tcaggtcatg gtatccgaga
    12181 tgaaattggg tcgctttatc ttgcattcaa aaacaccatt cgaacgcgtt tacgcgctac
    12241 cgccattaaa gccgattaca tccgcgaatc gatggatcaa agccgctcaa agcgtcaggt
    12301 tttaattctt gattgctgta acagcggtgc ttttcctccg ggtaccaagg ctgagcttgg
    12361 aggcgcaatg gggataacca gggcttttca aggttatggc cgcttcgtcc tgacagccag
    12421 cgacgcaact caattcgcat gggaaggaga tcaggtaata ggaacacaaa cagataattc
    12481 tctcttcaca cattttcttg tcaaaggcct agaaggtgaa gccgacaacg atggcgacgg
    12541 tcaaatcacc gtagatgaaa tttatgatta tgcctttcaa aagatttccc agattacacc
    12601 aaatcaaact cctaccaaat ccgcttccaa gcaggcgggt gaaattgtat tgcgcgagaa
    12661 tatgcggttt gaagatatca agcctgttgc tttgccggat gatttgatca gtgaaatcga
    12721 agacctccgt ccttctgtac gcgaatccgc agtacgatgg cttgaaacga ttgtcaaagg
    12781 caagaacatt ggcatggcgc gttcagcaat tgacgcgttg gaaaaagtcg cctcagatga
    12841 aaacacaacc cgccgcgtct cagaaatggc aatgagggtg atagagtctg ttcgtgcggc
    12901 ggagaagaaa gctgaggagg aacgaaaggc tcgagaggaa gcagaacgtc gcgcggctct
    12961 tgaagcagaa gaaaaacgtt tgacatttga aaaggcagag gctgaaagaa aagccaaaga
    13021 agaggcagaa aaattagcgc gggcaaaagc ggaaagagaa gcagccgagc aggaagccgc
    13081 acgcttgaaa gccgaacgcg aagaggctga aaaggcagct cgtgatgcca ccgaaaaagc
    13141 ttcaaaggag aaagctgaaa aggaggcttt agaccgcgaa accgcactaa aggcagagcg
    13201 cgacgcaatg gattttgcta a
//
LOCUS       DASTJP010000275         4719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1502963, whole genome shotgun sequence.
ACCESSION   DASTJP010000275 DASTJP010000000
VERSION     DASTJP010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502963"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFQ23_08310"
     CDS             complement(<1..517)
                     /locus_tag="VFQ23_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9906630.1"
                     /translation="MTEKTGERDPLVTVKDLHVEFDVRDGIVHSVDGASFNIYRGQTL
                     GIIGESGCGKSITAKAIMNMVPKPGKMRGEITFYQKSDDGQIIDQVRLDQTDPDGEII
                     RKIRGGHIAMIFQEPMSSLTPVYSTGFHLMEAIKLHRFLPAETIGQQMTSNLQKKRKV
                     TEEEARNIAIDM"
     gene            complement(535..1689)
                     /locus_tag="VFQ23_08315"
     CDS             complement(535..1689)
                     /locus_tag="VFQ23_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906631.1"
                     /translation="MSEAAAIPENTGAIPQAAKDNKKVSQSYWSLVWWKFRKNRLAVI
                     GAVILLIYYITCIFFAEFFAPYPKDLESDYFEARPTYFQFRDDAGNFSLRPFVYGLEE
                     KIDLKTRSRLYEYDKTVKYPIYFFVKGEPYKLLGFIPANIHLFGTDPANPDAKVFLLG
                     TDKLGRDQFSRILYGGRISLFIGLFSVAVFLFIGVTLGAISGYYGGTTDIVVMRITEL
                     LSAFPQEPLFLALGAAVPVAWPSTRVFFIVTILLALVQWGGLARQVRGLVLSGREEQY
                     VLAAQSFGASDRRIIFNHLIPATMSHVIVIATLKIPQMILLETALSFLGLGLVPPTVS
                     WGTLLQNANTIRAISFAPWYLFVVPFILFSILAYNMLGDGLRDAMDPYSK"
     gene            complement(1733..2719)
                     /locus_tag="VFQ23_08320"
     CDS             complement(1733..2719)
                     /locus_tag="VFQ23_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906632.1"
                     /translation="MGSFIIRRVFYAIIMLIVVSFVSFIIISLPAGDFMDQKIAELQA
                     RGDQSASQRVDEYRERYGLDKPVLTQYWIWISNFVQGDFGISFEFDRPVRELIGQRLG
                     LSIVLSVATLILTWAIAIPLGVYSAVNQYSVGDQVITAIAFIGLGIPGFLLALLILYF
                     AVVVLGQDAVGLFSREFADAPWSLAKVWDLLKHLWIPALIGAVTGTAGTIRIMRGNLL
                     NTFGQAFIEAARARGLKQNSVIWRHAVRMAMTPLIIILGTEALPGIIAGNLLVETVLN
                     LPTIGPLYIGALLTQDMYMAGTVLVLIVFLLLVGTLITDLVLAWTDPRVRLE"
     gene            complement(2890..>4719)
                     /locus_tag="VFQ23_08325"
     CDS             complement(2890..>4719)
                     /locus_tag="VFQ23_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9906633.1"
                     /translation="EERLPVKDDIMVVTPVDGIGEYGGIWHETTWWQGMGNIEMTVYD
                     PPVRWKADYTGYEPGLLKSWEISEDGKTLTWHFRQGIKWSDGVDFIPAVDLAYWWELA
                     TNEDFALVNIPFWGRDSDGTAMQVSFPDDYTMVMQWNEPHYIANFVVAQGFWEWLPME
                     RPKHFLSPHDPNYDSTKTYADLEKVVHSGDWLMNVAGYPCLHAWCPETVEPGVKTVLA
                     RNPYYWKVDTEGNQLPYIDYIDTVLIADAQARLLEVSQGKFEATFRGTDDPTNIPFLL
                     EQADANDYYLLEGAVNGAGGWPAWNINMNFNDCETYPDTCEEIRALLQNQSFRQGLAY
                     ALDRERLIDVAWGGIGEPTAFTISPQAWHFASPEGQAVYEEWKSTYSEYDPELAAQKF
                     DEAGFVDADGDGWRDLPSGAPFELVMDQGDWGGQVIPVVSNETYAANLEAVGVNTLIN
                     DLMGQPDWTLRHSQGLFMIRNSHASELDVWTFPDWIFPLRGTEARAWPLEGRYYETGG
                     AEGWEPEPGTTYAYELQQLYKRGIAEPDIEARHQIVYEAIRILIDNGPFIIGGAGDQQ
                     MPVVVRNGFHGIPDLVILGPWAPGSPGNLSPEQFWMEESLRND"
BASE COUNT         1177 a         1244 c         1175 g         1123 t
ORIGIN      
        1 gcatatcaat tgcaatgttc ctcgcttctt cttccgttac cttgcgtttc ttctggagat
       61 tactcgtcat ttgttggcca atggtttccg cgggcaagaa ccggtgcagc ttgatagctt
      121 ccataaggtg gaacccggta ctgtaaaccg gcgtgagtga actcatgggt tcctgaaaga
      181 tcatcgcgat atgaccacct ctgattttgc gtatgatctc gccatccggg tcggtttgat
      241 cgaggcgaac ttgatcaatg atctgaccat catccgattt ttgatagaag gtgatttcac
      301 cgcgcatctt gcccggcttt ggcaccatgt tcatgatcgc ttttgcggta atggattttc
      361 cacaaccact ttccccgatg atcccgagcg tctgcccgcg gtagatgttg aaactggcac
      421 catccacgga atggacgatg ccatcccgca catcaaattc aacatgtaaa tctttgaccg
      481 ttaccagagg gtcacgttcg cctgtttttt cagtcattgg ttttactcct ctgtttattt
      541 actgtagggg tccattgcat cgcgcagacc atcacccagc atgttgtaag ctagaatgga
      601 aaacaggatg aaaggaacga cgaacaaata ccagggcgca aagctgatcg ctcggatcgt
      661 gttggcattt tgaagcaggg ttccccagct taccgtggga ggcaccaatc ccagtcccag
      721 gaaactgagg gctgtttcaa gcagaatcat ttgtgggatt tttaatgtcg caattacaat
      781 gacatgactc atcgtagcgg gaatcaggtg attgaagatg attctgcggt cactggcccc
      841 aaagctttgc gccgccaata catattgttc ttcgcgtcct gagagaacca aacccctgac
      901 ctggcgagcc aacccgcccc attgaaccaa cgccagtaga atcgtcacga tgaaaaacac
      961 acgcgtggat ggccatgcga caggtacagc ggcacccagt gccaggaaga gaggttcctg
     1021 agggaaggct gataataatt cagtgatccg catgacaact atatcggtgg tcccaccgta
     1081 ataaccagaa attgctccta aggtaacacc tataaacaaa aagacagcaa cgctaaacag
     1141 accaataaac agggagatgc gaccgccata taaaatacgt gaaaactggt cacgtcccag
     1201 cttgtctgta cccaacagaa acactttagc atccgggttg gcggggtctg ttccaaagag
     1261 atgaatattt gctggaataa accccaaaag tttatagggt tcacccttta caaagaagta
     1321 aatcggatat ttgaccgtct tgtcgtattc ataaaggcgc gagcgcgttt tcaaatcaat
     1381 tttctcttcc agaccataaa caaatggccg cagagagaaa ttccctgcat cgtctctgaa
     1441 ctgaaaatag gtgggccgcg cttcgaagta atcgctttca agatctttcg ggtagggggc
     1501 gaaaaattct gcaaaaaaga tgcacgtgat gtaatagatc agcaaaataa ccgccccaat
     1561 aaccgccaga cgatttttgc gaaatttcca ccaaaccagg ctccaatagc tttggctgac
     1621 tttcttatta tctttggccg cttgagggat cgctccagta ttttcaggga ttgctgctgc
     1681 ttcactcatg gttcacctcc gatatatttt tgtggggatc ggatttagaa atttactcaa
     1741 gtcggacgcg cggatcagtc caggccaata ccaggtctgt gatcaatgtg ccaaccagta
     1801 gcaggaagac gatcagtacc aaaaccgttc cagccatata catatcttgt gtgagtaatg
     1861 cgccgatgta aagggggcca atcgttggca ggttaagcac ggtctcaacc agaaggttgc
     1921 cagcaatgat ccccggcagg gcctcggtgc ccaaaatgat gatcaatggg gtcatggcca
     1981 tgcgtactgc atgccgccag atcacactgt tctgttttag accacgagcc cgcgcagcct
     2041 caatgaaagc ctgaccaaag gtgtttaaca aattaccgcg cataatgcgg atagttccgg
     2101 cggttcctgt taccgcgcca atcaacgcag gtatccagag gtgttttagc aaatcccaaa
     2161 cctttgccag gctccaggga gcgtctgcaa actcccgtga aaacaagccc accgcatctt
     2221 gtcctaggac aaccacagca aaatataaaa tgagcagcgc taacaaaaaa cctggtatgc
     2281 ccagtccaat aaatgcgatg gccgtgatga cttggtctcc gactgaatat tggtttaccg
     2341 ccgaatatac acccagcggt atggcgatgg cccaggttaa gatcagtgtt gctactgata
     2401 aaacaatgga taaaccaagg cgctgaccga ttaattctct cacagggcga tcaaactcaa
     2461 aggatattcc aaagtcaccc tgaacaaaat tgctgatcca gatccaatac tgggtgagca
     2521 cgggtttgtc cagaccatag cgttcgcgat attcatcgac gcgctgtgat gcactctggt
     2581 caccacgggc ttgcaactcg gcgatttttt ggtccatgaa gtcgccggcc ggcaaagata
     2641 tgatgatgaa gctcacaaat gagacaacga tcaacatgat tatggcgtag aaaacacgcc
     2701 gtatgatgaa cgaacccatg actcatcctc ctctctgcgt tagccgcaaa gaaaaatgaa
     2761 atcgatcttc catctgcaac ttgggagggg caaccctgcc ttgtcaggcg agcagctccc
     2821 gttttttaac ttatctacga agatggcagg tacactcaag tgcacctgcc atcttaagct
     2881 taccggatat taatcgttgc gaaggctttc ttccatccag aattgttctg ggctcaggtt
     2941 gccagggcta ccgggtgccc acggtccgag gatgactaga tctgggatgc cgtggaaacc
     3001 attgcggacg actacaggca tctgctgatc gccagcgcca ccgataatga aggggccatt
     3061 gtcgatgagg atgcgaatcg cctcatagac aatctggtgg cgtgcttcaa tatctggttc
     3121 cgcaatgccc ctcttgtaca gctgttgcag ttcatacgca taagtggtgc caggctctgg
     3181 ctcccaacct tctgcgccac ccgtttcgta gtaacggcct tcgagcggcc aggcgcgagc
     3241 ttccgtgcct cgcagcggga agatccagtc tgggaaggtc cagacgtcca gctcagatgc
     3301 gtgtgagttg cggatcatga acaaaccctg gctgtgtcgt aaagtccaat caggctgacc
     3361 catcaggtcg ttgatcagtg tgttaacgcc gactgcttcc aggttggccg cataggtttc
     3421 gttcgagacc acggggatca cctgaccacc ccaatcgccc tgatccatca ccaattcgaa
     3481 tggtgcaccg ctgggcaggt cgcgccagcc atcgccatcg gcatctacaa agccggcttc
     3541 atcaaatttc tgtgcagcca gttcgggatc atattcacta taggtggact tccactcttc
     3601 gtagacagcc tgaccttcag ggctggcgaa gtgccatgct tgtgggctga tggtgaaggc
     3661 agttggctca ccgatgccgc cccacgccac gtcaatcaga cgttcgcgat cgagcgcata
     3721 ggcaagaccc tggcggaagc tctggttctg cagcaaggcg cggatctctt cacaggtgtc
     3781 aggataggtc tcacaatcgt tgaagttcat gttgatgttc cacgcgggcc aaccaccagc
     3841 gccattcacc gcaccctcaa gcaggtagta atcattagca tccgcctgct ccagcaggaa
     3901 ggggatgttg gtggggtcat ctgtgccgcg gaaggtggct tcgaacttgc cttgcgaaac
     3961 ttccagcagg cgtgcctgag catccgcaat caatactgta tcgatgtagt cgatgtacgg
     4021 cagttgattg ccctctgtgt ccactttcca atagtagggg ttgcgtgcca gcacggtttt
     4081 tacgcccggc tcaacggttt ccgggcacca ggcatgcagg caggggtacc cggcaacgtt
     4141 cattagccaa tcgccactat gaacgacctt ttccaagtct gcatacgtct tggtgctatc
     4201 gtagttggga tcgtggggag acaggaagtg cttgggacgt tccattggca gccattccca
     4261 gaagccctgg gcgacaacga aattggcgat gtaatgcggc tcattccact gcatgaccat
     4321 cgtatagtca tcggggaatg acacttgcat tgcggttcca tctgaatctc taccccagaa
     4381 cggaatgttg acgagcgcga agtcttcatt ggtcgcaagt tcccaccaat aggccaggtc
     4441 gactgcgggg atgaaatcca cgccatcgga ccatttgatg ccctggcgga aatgccacgt
     4501 gagggtcttg ccatcctcag agatttccca tgacttcagc aagccaggct cataaccggt
     4561 gtaatcagct ttccagcgaa ctggtgggtc atagacagtc atttcgatat tgcccatgcc
     4621 ttgccaccag gttgtttcgt gccagatgcc gccatattcg ccaatgccgt caaccggggt
     4681 cacaaccatg atgtcatctt taacgggcaa ccgctcttc
//
LOCUS       DASTJP010000276         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1195241, whole genome shotgun sequence.
ACCESSION   DASTJP010000276 DASTJP010000000
VERSION     DASTJP010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195241"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..390
                     /locus_tag="VFQ23_08330"
     CDS             124..390
                     /locus_tag="VFQ23_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906634.1"
                     /translation="METDPNSLDPELRNARFFAIASPALGLLSLCVAIIPACGGVASV
                     LGIILGLASLKTEHSRAAIAGVAISILGMLITITYALFLYFFKQ"
     gene            complement(406..2010)
                     /locus_tag="VFQ23_08335"
     CDS             complement(406..2010)
                     /locus_tag="VFQ23_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9906635.1"
                     /translation="MRKRIFNHLFIFLLLAACVPATPTLAPTATVAPTPTPAPEVPQL
                     PWWREAVFYEIFVRSFYDTDGNGIGDFNGITQKLDYLQELGINAIWLMPIHPSPSYHG
                     YDVINYYAVNSEYGSMDDFKHLLEEAHNRGIRVIIDFVINHTSSQHPWFQDSNRDLES
                     KYRNYYIWSADGGNGWHQGQNGYYYAFFCDCMPDLNYNNPDVTTDMLKVTDYWLSEIG
                     VDGFRVDAAKHLIEDGEVRENTPATHEWYKGFYIAYKEQNPQAYAVGEVFGAGASVVK
                     SYTGDQLDQVFNFEMSTGFVNSVNGGANSGVTSAIKFTLQDMPDFNFATFLTNHDQNR
                     AMSVFNGDPGKAKAAAALLLTSPGTPFIYYGEEIGMQGQKPDEDIRLPMQWSAEANAG
                     FTTGMPWRAPASDYPQVHVAAQNEDPNSLLNHYRTMISLRRASPALSGGELVLVETDN
                     PGVYAVLRSSPDQTLLVIVNLEGTPISDYQLSLNESLLSDGTVTPQPLFGTIEAMPVI
                     ISGGKFSDYKPMNELLPYQSYILELE"
BASE COUNT          516 a          541 c          627 g          571 t
ORIGIN      
        1 ctgtgtgcga aaacccagtg cgacacccag tgccagcagg atggcaagca gatagagaat
       61 gtttcggatg gatttgttca tggatgagga tagtataatt cgattcgttt gcaggaattt
      121 tcaatggaaa cagatcccaa cagccttgac ccagaactgc ggaacgcaag attttttgcc
      181 attgcatccc ctgcccttgg cttgctcagc ctgtgcgttg ccattatccc tgcttgtggg
      241 ggagttgcga gcgtgctggg gattattctg ggattagcca gcctgaaaac agagcacagc
      301 cgagctgcca ttgcaggtgt cgcgatttcg atcttgggca tgttgatcac gatcacgtac
      361 gcactgttcc tatacttttt taaacaatga ttgaatgacc acgcctcatt caagttctaa
      421 gatatacgac tggtagggga gcagttcatt catcggtttg taatcactaa attttccgcc
      481 cgaaattatt accggcatcg cctctattgt accaaacagg ggttgaggtg tgacagtgcc
      541 gtccgagagc aggctctcgt tcaggctcag ttgataatct gagattggcg ttccttctag
      601 attgacaatc acaagcagcg tttgatccgg ggaactgcgc aaaacggcat acacgccggg
      661 attatccgtt tccaccagga ccagctctcc gccgctcaat gccgggcttg ctctgcgaag
      721 gctgatcatt gtgcgatagt gatttagcaa ggaattggga tcttcatttt gtgcagcgac
      781 atggacttgc gggtaatccg atgcaggcgc gcgccagggc atgcctgtcg taaaccctgc
      841 gttcgcttct gcgctccact gcataggcag acggatatcc tcatcgggtt tttgtccctg
      901 catgccaatt tcttcgccgt aatagataaa gggtgtaccc ggggaggtaa ggagcaatgc
      961 tgccgcggct ttggcctttc ccggatcccc gttgaaaacg gacatggctc gattctggtc
     1021 gtgattggta aggaaagtgg caaagttgaa atcaggcata tcctgcagtg taaatttgat
     1081 cgcgcttgtc acgcctgagt tcgcgccgcc attcacgctg ttgacaaatc cggtggacat
     1141 ttcgaagttg aagacctggt cgagctggtc gcctgtgtac gactttacaa ccgacgctcc
     1201 cgcgccaaat acttcgccga cggcataggc ttgagggttt tgttccttgt aggcgatgta
     1261 aaaacctttg taccattcat gcgtggcggg cgtgttctca cgaacctcgc catcttcgat
     1321 cagatgcttg gcggcgtcca cgcggaagcc gtccacgccg atctcgctga gccagtaatc
     1381 ggtcactttg agcatatcgg tagtgacatc cgggttgttg tagttcaggt caggcataca
     1441 gtcgcagaag aaggcgtagt agtagccgtt ctgtccctgg tgccagccat tccctccgtc
     1501 cgctgaccag atgtagtaat tgcgatactt tgattcaagg tccctgtttg agtcttgaaa
     1561 ccacgggtgt tgactcgatg tgtggttgat cacaaaatcg atgatgacgc ggatgccgcg
     1621 gttgtgagct tcttcaagga gatgcttgaa atcatccatg cttccgtatt cggaattgac
     1681 cgcgtaatag ttgatcacat catagccgtg atacgacggg gatgggtgaa taggcatcag
     1741 ccagatagcg ttaatcccca gttcctgcag atagtccagt ttctgggtga tgccgttgaa
     1801 gtcgccgatg ccgtttccat ctgtgtcgta aaaactgcgg acgaatattt cgtaaaatac
     1861 cgcttcacgc caccagggga gctgcggaac ttcgggagcg ggagtcggcg tgggagcgac
     1921 agttgcagtg ggagcaaggg tcggcgttgc ggggacgcag gcagccagga ggaggaaaat
     1981 aaagaggtgg ttgaaaatcc gcttgcgcat aggtgtctat aaaaaatctt tcctctttct
     2041 tctttagtag ggaagaaaga ggaaaaaaga aagagagcca ggtgaatggg cgtaggcttg
     2101 cttagccttt cacaccgccg gcagtcaacc cactgacgat ctgcttttgc agtgcaatgt
     2161 aaatgatcgc aaccggcagt gccacgatga tcgccatcgc cgcgaagcga ttccaaggga
     2221 tgctattggc atattgaccg gtcatgttgt acagg
//
LOCUS       DASTJP010000277         5738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_953113, whole genome shotgun sequence.
ACCESSION   DASTJP010000277 DASTJP010000000
VERSION     DASTJP010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953113"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFQ23_08340"
     CDS             complement(<1..758)
                     /locus_tag="VFQ23_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906636.1"
                     /translation="MPGREDIFQKAMNEGHSAAWDQEWSKAAGAYRKALQEMPDNPKA
                     LNSLGLALFQQAQYEEALQIYQRVAQIAPQDPVPMEKLAQLLERTGHLKEAIEAAFRA
                     ADLFLNQRDVDKAIENWVRVTTLDPEHVQAHSRLALAHERLGHKSQAVTEYIAVASLI
                     QRTGNAQKTAELVTKALQILPESAEAAQAQTLLRTGQLLPKPLRPKGGTGPIAMAQVK
                     QLEKPKQEADSGLDPIAEARKKALTRLAEILFEYT"
     gene            complement(803..3598)
                     /gene="polA"
                     /locus_tag="VFQ23_08345"
     CDS             complement(803..3598)
                     /gene="polA"
                     /locus_tag="VFQ23_08345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET9906637.1"
                     /translation="MPPTLYLIDGHALAYRMYFALTAGGGSARWQTSKGEPTAGTYGF
                     ARELLRIIEQEKPDYLAVAFDTGKTFRDELFPEYKGTREKMPDDLRPQIERIRQVVDA
                     FNIPRLEMEGFEADDVLGSIARIAANEGLGVKIITGDRDLLQLVNERTTVYLAGDDQN
                     YISDEDVTRKLGVPPAQVVDYKALVGDKSDNIPGVAGIGEKTAISLLQKFGTLDSIYQ
                     SIDQVENRWKGKLEQNKENAYLSRDLAQIKTDLNIKFDLERAKTDGLDFPSIESIFRD
                     LEFRSLLKTLEKLNGNETAVSTTTSAPLKPGQQMSMFVNEPQVVYAPTNNLNIEVIVV
                     DTDKKLVNLVKELNKAKVISFDTETTSTEEMKAEIVGISLAIKEGEGYYIPTGHLAGT
                     NLPLDQVISALQAPLTNAKIGKIAHNAKYDYIVLARHGLKVSPLTFDTMLAEFIIDPS
                     SRSMGLKNLAFVKLGEEMTHIEELIGKGKKQITMAEVAIEAAAPYAVADAEVTLRLLP
                     IQQEGLKRVHGEKLLEEIDLPLTPVLADMEMSGISLDLPFFEETSRRLEKRMAEIEKQ
                     VFEMVGKPFNINSTQQLSDILFQRLGLEPPDRGNKTASGHFSTSAGVLDLLRGKHAVV
                     DLILEHRELSKIKSTYVDALQAALNPDTGCVHTSYSQIGAVTGRLSSSNPNLQNIPIR
                     TEEGRRIRNGFVAGKDSVLLSVDYSQIELRIVAHMAEDEGMLAAFRADEDIHATTAAA
                     IYNVKNEAITKNMRRHAKAINFGLIYGMSVFGLTRTTELTLAEAETFVKAYFEKFPGV
                     KKYLDGIRKQAAKQGYVETLLGRRRYFPALQGKANVQVKNREEREAINAPVQGTAADI
                     MKIAMLKIPPALKAAGLKAKMLLQVHDELVFEVPEKELKKTAEVIQEVMTNAYPLSIP
                     LSTEARYGKNWGEMKTI"
     gene            complement(3835..5457)
                     /gene="tnpA"
                     /locus_tag="VFQ23_08350"
     CDS             complement(3835..5457)
                     /gene="tnpA"
                     /locus_tag="VFQ23_08350"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HET9906638.1"
                     /translation="MTHSLLLVSADTSFAQLLLHGLEQEGYLVHIVKGKGEAVVRADE
                     ANCSLAFLDMDLGYRAVLDIGGALRMLNPGIRFIIFAREGTPAAPDELRPWLLSPKPY
                     FLPDVLNMLNNKSTPISQPIRKAQAAVSNSPSQVQTSNPALPWLQDVTKAAQHLTRLT
                     LESSAQAALITRGDNLWAYAGQLSQGAAKELAVTVTRHWDGQKGSDLLRFVRLEATKA
                     EHMLYATRIADDVVLALVFDAETPFSTIRTQAGQLAQSLSIPGAETTETPVEPKAVVA
                     SYDAPTEYIDEDEDGMGFPNISDILSDVPPPIPTGTSRQMEEEDNWGTRPSPARSNQY
                     SRESSPAVRVDDLLIANQNNEQTVEHVVQDFDATMPSKSRVRPETPIRRPMPGELDET
                     RPHSITEVAGRVMLEPISPGLYNLTYACLLVPRFSSHYLTGDMSDRLSEWLPQICIAF
                     GWRLEFLAVRPEYVQWVVNVPPATSPGYLMRIMRQQTSEKIFTEFTRMKKENPSGDFW
                     APGYLIMGGTQPHPPQLVKDYIKQTRTRQGYS"
BASE COUNT         1318 a         1435 c         1436 g         1549 t
ORIGIN      
        1 gtgtattcga agagaatctc tgccagccga gttaacgcct ttttgcgcgc ttccgcgatc
       61 gggtccagcc ctgaatcggc ctcctgtttc ggcttctcca attgcttgac ctgcgccatc
      121 gcaatcggcc ctgtcccgcc cttgggtcgc aggggtttgg gcagaagttg acccgtgcgt
      181 aaaagtgtct gtgcctgggc agcctccgcg ctttcaggga ggatttgcaa agccttggtt
      241 accagttcag ctgtcttctg ggcattgccc gtgcgctgta taaggctggc gaccgcgata
      301 tattctgtga ccgcctgtga tttgtgtcca agccgctcat gggccagggc cagccgagag
      361 tgtgcctgca catgttcagg gtcgagggtt gtgactcgca cccagttctc aatggcttta
      421 tccacatcgc gttggttgag gaaaagatca gcggctcgaa aggcagcctc aatcgcttcc
      481 ttgagatgac ccgtgcgctc aagaagctga gccagttttt ccataggcac tgggtcttgc
      541 ggggcaatct gcgcgacgcg ctgatagatc tgcaatgcct cttcgtactg cgcctgctgg
      601 aataatgcca aacccaaact gttgagagct tttgggttat ccggcatttc ctgtaaagct
      661 ttgcgatagg ccccagcagc cttactccac tcctggtccc aggcagcgga gtggccctcg
      721 ttcattgctt tttggaatat gtcttctcgt ccgggcatag gttgaattat atcgtaggtc
      781 accttgcaaa cgtgacctac atttatatcg ttttcatttc accccagttt tttccatagc
      841 gcgcctcggt ggaaaggggg atgctgagcg gataggcatt cgtcatcacc tcctgaatga
      901 cctcggctgt ctttttcaat tccttttcag gaacctcaaa tacaagttca tcatgtacct
      961 gcaaaagcat cttggctttc aagccggctg ctttaagcgc aggaggaatt ttcaacatcg
     1021 caatcttcat gatgtctgct gcggtaccct gcacaggggc attgatcgct tcgcgctctt
     1081 cacggttctt cacctggacg ttggctttgc cctgcagcgc cgggaagtat cgtctgcgcc
     1141 caagcagtgt ttccacgtat ccttgtttcg cagcttgctt gcggattcca tcgaggtatt
     1201 ttttgacccc ggggaatttt tcgaagtaag ctttgacaaa cgtctcggct tcggcaagtg
     1261 tcagctcggt tgtgcgcgtg agaccaaaca cagacattcc atagatgaga ccaaaattga
     1321 tcgccttcgc gtggcggcgc atgttcttag tgatcgcctc atttttgacg ttgtaaatgg
     1381 cggccgcagt tgtggcatgg atatcttcat cggcccggaa tgccgccagc atgccttcat
     1441 cttcagccat atgtgccacg atccgcaatt cgatttgtga atagtcaaca gagagtaaca
     1501 cgctatcctt gccagcgaca aacccattac gaatacggcg cccttcttcg gtacggatcg
     1561 ggatgttctg caaatttgga ttcgatgacg ataaacgtcc cgtcaccgcg ccgatttgac
     1621 tataggatgt atgaacacaa cctgtatctg gatttagcgc ggcttgcagg gcatctacat
     1681 acgttgactt gattttcgaa agttcgcgat gttccaggat caaatccact accgcatgct
     1741 tgccccgcaa taaatcgagc acacctgcgg atgtggagaa atgtccgctg gcagttttgt
     1801 tgcctcgatc cggtggctcc agccccagcc gctgaaagag gatatccgaa agctgttgag
     1861 tcgaattgat gttgaatggt ttcccaacca tctcaaagac ctgcttttcg atttcagcca
     1921 ttcttttttc aagtcgcctg gaggtttcct caaagaaggg caggtcaaga gaaattcctg
     1981 acatctccat atctgcgaga acgggcgtga gaggcaagtc aatttcttcg agcagttttt
     2041 caccatggac gcgtttcaat ccctcctgct gaatgggaag caggcgcaac gtgacttctg
     2101 catctgctac cgcataaggc gctgcggctt cgatggcaac ctctgccatg gtaatctgtt
     2161 ttttgccttt gccgatcagt tcttcaatgt gtgtcatctc ttcgcccaac tttacgaagg
     2221 ccagattctt tagacccata ctgcgtgaag acggatcaat gatgaattct gccagcatcg
     2281 tatcgaaagt cagcggggag acttttaatc catgcctggc caggacaata taatcatatt
     2341 tcgcgttgtg tgcgatcttg ccaatcttgg cattcgtcaa aggagcctgg agtgcagaaa
     2401 taacttgatc aaggggtagg ttggtcccgg ccaaatgacc tgtggggatg taatatcctt
     2461 caccctcttt tatagcgagc gagataccaa caatctccgc tttcatttct tcagtggaag
     2521 tggtttcagt atcgaaggaa attacttttg ctttattaag ttctttgacg agatttacaa
     2581 gttttttatc tgtgtcaact acgatgactt cgatgttcaa attgttggtt ggagcgtaaa
     2641 ctacctgcgg ttcatttaca aacattgaca tttgctgccc gggtttcaga ggggcagacg
     2701 tagtcgttga gaccgcagtc tcatttccat ttagcttctc cagagttttg agaagcgaac
     2761 ggaattccaa gtccctgaaa atcgattcaa tcgaaggaaa atccaatcca tcggttttgg
     2821 cgcgttcaag gtcaaatttg atgttgagat ccgtcttgat ctgagccagg tcacggctta
     2881 gataggcatt ctctttattc tgttcaagtt tgcctttcca gcgattctca acctggtcaa
     2941 tgctttgata aatgctatcg agtgtgccaa atttctgaag cagggaaatc gcggtctttt
     3001 ctccgattcc tgccacaccg ggaatgttat cggacttgtc accaaccagg gctttgtagt
     3061 caaccacttg agcaggcggt acccctaact ttctggtcac atcttcatca gatatgtaat
     3121 tctggtcatc gcccgccaga taaactgtgg tgcgttcatt gaccaactgc aatagatctc
     3181 gatccccggt gatgatcttg acccctagtc cctcatttgc cgcaatcctt gcaatcgagc
     3241 cgagcacgtc atctgcctcg aagccttcca tttccaggcg cgggatgttg aacgcgtcca
     3301 ccacctgacg aattcgctcg atctgtggac gcaggtcatc aggcattttt tcacgtgttc
     3361 ccttgtattc agggaagagt tcatcgcgaa acgtcttgcc agtatcaaag gcgacagcca
     3421 ggtagtccgg tttttcctgt tcgataatgc ggagcagctc acgcgcgaag ccgtaggtgc
     3481 cagcagtcgg ttcacctttc gaagtttgcc agcgagcact gcctccgcct gctgtcaacg
     3541 caaaatacat gcggtaggct aaagcatgtc catcaattaa atagagagta gggggcatgt
     3601 tgggtccagt cgttagtagt tacgaagtgc cgcatcacta actaaactcg aactttaatt
     3661 tttcggcata caaaatcgtt caaaaaactc agttagtaat gagacaagct gtagtgtaca
     3721 ggtatagtta aatagaaacg cggaggtatt atactccgcg cttattagat aattgtcatt
     3781 cgaaagattt caacctgacg atgaaatctt ctattaaggt tgtttatctt ttggttatga
     3841 atacccttgt cgcgtgcgag tctgcttgat gtaatctttt accagttgtg gcggatgtgg
     3901 ctgtgtgcca cccatgatca ggtacccggg agcccagaaa tcacctgacg gattttcctt
     3961 tttcatacgg gtaaactcag tgaatatttt ttcagaggtc tgctgacgca tgatacgcat
     4021 caaatacccg ggagaggttg caggtggaac attcacaacc cactgtacat attcaggtct
     4081 gactgctaga aattccaacc gccagccaaa ggcgatgcag atctgcggca gccattcaga
     4141 aagacggtca gacatgtcac cggtcaagta atgtgagcta aaacgcggca ccaatagaca
     4201 ggcataggtc agattgtaaa gtcccggtga gatcggttcg agcatgacgc ggcctgccac
     4261 ttctgtaatc gagtgtggac gggtctcatc caattcacca ggcataggtc ggcggatcgg
     4321 cgtctcgggc cgcacccgtg actttgaagg catggtcgca tcaaaatcct gaacgacatg
     4381 ttcaacggtc tgttcgttat tttgattcgc gatgagcagg tcgtccacgc gcactgccgg
     4441 tgaactttcg cggctatact gattcgaacg tgccggggaa ggacgcgtac cccaattatc
     4501 ttcctcttcc atctgcctgg atgtgccggt cggaatcggc ggtgggacat ccgaaagaat
     4561 atctgaaata ttcggaaagc ccatcccatc ttcatcttcg tcaatatact cagtgggggc
     4621 gtcataagat gcaaccactg cttttggttc aacgggtgtt tctgtggtct ccgcacccgg
     4681 tatggaaagt gattgtgcca attgaccggc ttgcgtgcgg atcgtgctaa atggagtttc
     4741 tgcgtcaaat acgagagcga gtaccacatc atctgctatg cgcgttgcat aaagcatatg
     4801 ttcggctttt gtagcttcca aacgaacgaa acgcagaaga tcactgcctt tttgaccatc
     4861 ccagtgacgg gtgactgtga ctgccaattc tttggccgcg ccctgggata actgacctgc
     4921 atacgcccat aagttatcac cccgcgtgat caacgcggcc tgcgccgatg actcaagggt
     4981 cagccgtgtg agatgctgcg cagccttggt gacatcctgc agccacggca gcgcgggatt
     5041 actcgtctga acctggcttg gggaattcga tacagcagct tgcgcttttc gaatgggctg
     5101 agaaatgggt gtagatttgt tattcaacat attcaatacg tccggtaaaa aatatggctt
     5161 gggggagagc agccagggtc tgagttcatc tggggcggcc ggtgtaccct cacgagcaaa
     5221 gatgataaac cggatgcctg ggttgagcat acgcagcgct ccgccgatat ccaggaccgc
     5281 tctgtatcca agatccatat caagaaaagc aagtgagcag ttcgcttcgt ccgcgcggac
     5341 aactgcttcg cctttccctt tcacgatatg aacaaggtag ccttcctgct ctaaaccatg
     5401 gagcagaagc tgtgcgaaac tcgtatctgc gctcacgagg agcaggctgt gggtcatgct
     5461 tggcaaagtg tatcatggca taagtgcata ggcaagcatg atgttgtaac gcaccttacg
     5521 aaaatgaaat tctgcccaat gttgtctata caacttgatt ttcaggtcta tttgtgctat
     5581 caatagaaaa aattttcacg agggttttga atgcaccgcg aacgggtttc tgaaaacgta
     5641 ttttggttcc aaagtgaaat ttatgcacag gtgacagccg gagttgttgc aggtccgcaa
     5701 tgggcagtgg ttatcgacac ccttgccctc ccagacga
//
LOCUS       DASTJP010000278         5929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_325541, whole genome shotgun sequence.
ACCESSION   DASTJP010000278 DASTJP010000000
VERSION     DASTJP010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325541"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2053)
                     /locus_tag="VFQ23_08355"
     CDS             complement(65..2053)
                     /locus_tag="VFQ23_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906639.1"
                     /translation="MSMPGDLLKMFEKVEGLNYGTRFYKADLHFHTPASEDARGRNRY
                     NFNPYKIQYPKRAGNPNYEEQVNAIHAGILKEARVIASNIVQRFLELGLSIVAVTDHN
                     AIGTIWADKESEKRLMDLAAPTWYELIDDQAQIVNNAVGRTELLVLPGVEISTTGVHI
                     LAIFPPQNPRRKVHFMICDLLAEVGFGVDMWGKNPEAGNASVYDALELILQKGGLAIP
                     AHIDGSDQALLDLYKLTSDSMENVLQSKDLRAVEIVDPAKFSRPNKKIKTSLKAKFDA
                     MRRKEGLPYLAYFQGSDAHDLASIAKRFTYLKMTEPTFAGLQTAIKMPSSRVRIEDSF
                     KQLDGLYLHGMVFDHPSLGSQSLHFNHNLNCIAGKLGSGKTILFDMIQGVVNPEFPTM
                     DGNAILFVEKNTGDVCHYFAYARKKGSSEVSVFAIDPMAKTVTQLTLELAKAAAIQPK
                     FYNSKQMEKIINDPAELQAFLQKNFGAPTPDNVSKFNDRFSSPRFLDEKRDPLLMLAL
                     DGAGYKLAMNLCWRRDDICLADFSNLNASQRKTAMMCMIILSSQFGPVMIDAPEAEFD
                     HEDMSKFLVPVIQEHKDAHQILLFTNHPMLAVNTDPDNYLLMQSQAGKRNKIVSGFAI
                     DAVGAQKDLLLNILEGSLETFRKRASRYEYRLKFGVPR"
     gene            2291..2683
                     /locus_tag="VFQ23_08360"
     CDS             2291..2683
                     /locus_tag="VFQ23_08360"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9906640.1"
                     /translation="MNNEVNHVKIPRLRRPETIPLGLKIPVGKSLRSAIQKVVEELKP
                     EKIILFGSYAYGTPSPHSDVDLLVIMKTKASLKDRSWAVSRLLLPRPFPVDILVKTPK
                     EITKALESGDFFLKEIMTRGKVLYDRNQ"
     gene            2667..3050
                     /locus_tag="VFQ23_08365"
     CDS             2667..3050
                     /locus_tag="VFQ23_08365"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HET9906641.1"
                     /translation="MTEINDPIAWAERAEEDFILARTALRRKQPLTYGACFHAQQCAE
                     KYMKALLISKGADFPKTHDLLMLNGLCSSNGIFLEIDSKHLNTLTDYAVRTRYPGNDP
                     TVDDSKEALELAKLIRTFARKYLGL"
     gene            3207..4136
                     /locus_tag="VFQ23_08370"
     CDS             3207..4136
                     /locus_tag="VFQ23_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9906642.1"
                     /translation="MEHLQIAYDLFVIMIGLAALSIVVFWAARTGESDLRNFSILYTC
                     FTLVLIVELLEKYLFLNVEDYSARLWYALSGIGQGLNIAVIVAAIHFFAGVYQVRYQR
                     AITIFFLVVMLVSLVLILSPIGAVLNAEARSISFRIGFKIASVFYFVAFTFMMALGYG
                     LLRRAWQTERRNFLIGLLLFATVGFIETTLSLPVALRRESVTISTGSNFLYSSIPYAL
                     YGIFLIFYFLRYPALAPLEMDDLSATFLSKYGITDREREIILKVVQGKSNADIASELF
                     ISLATVKTHLHNIYRKIEVDSRYDLLARVRSGQ"
     gene            4516..5238
                     /locus_tag="VFQ23_08375"
     CDS             4516..5238
                     /locus_tag="VFQ23_08375"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HET9906643.1"
                     /translation="MKVQTTNPGKPTIGKVIGWTIMLLAATMPVLVSLGYLTLDPDNF
                     PFTQQKAVYTAHKTMLITHIIASMIAILIGPFQYLPGIRKGRFLKVHRWLGRTYLLSV
                     LFGGVSGLYMAQFAHGGIITELGFGALAVLWLYSGYRAYRHIRNKDIESHRQWMIRNY
                     ALTFAGVMLRVWMPLSVSLGADFTSAYIIVAWACWVPNLLVAEWIIRGSRRSSRRVGP
                     SGATVRVQKTAQINSEENVYAD"
     gene            5317..>5929
                     /locus_tag="VFQ23_08380"
     CDS             5317..>5929
                     /locus_tag="VFQ23_08380"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9906644.1"
                     /translation="MNTLLMKKSLAASITILLTLGILLSIVGPVKAHKTATALDSNVS
                     TMPSQELGVTDPAELENFLDGLLTNEMEENHIAGAVISVVKDGKLLFAKGYGYADIEN
                     GIPVDPEQTVFRIGSVTKLFTWTAVMQLAEQGKLDLDADINTYLDFRIPDTYPQPITL
                     THLLTHTPGFEDRFFEVLASDVNDLVPVREWLVSHMPARVRPPG"
BASE COUNT         1415 a         1530 c         1456 g         1528 t
ORIGIN      
        1 gtaaatattg aattagctgc gtacatctta aacagcgcaa tcgttttatt cgtggtttcg
       61 tcagtcacct gggtacccca aacttcaacc tgtattcata gcgactagcg cgcttgcgga
      121 aggtctccag gctgccctcc aggatgttga gcaataaatc tttctgcgcc ccgaccgcat
      181 caatggcaaa gccggagacg atcttgttcc gttttccagc ctgcgactgc atcaacaggt
      241 aattatcagg atcggtattg acagccagca tgggatggtt ggtaaagagc aggatctggt
      301 gcgcgtcctt gtgttcctga ataacgggta cgaggaattt cgacatatct tcgtggtcga
      361 actccgcctc aggagcatcg atcatcaccg gcccgaactg gctgctcagg atgatcatgc
      421 acatcatggc cgtcttgcgc tgactcgcat tcaggttcga gaagtctgcc aggcagatgt
      481 catctctgcg ccaacacaaa ttcatggcga gcttatatcc cgccccgtcc agggccaaca
      541 tcagcagtgg gtcccgtttt tcgtccagaa aacgcggact ggaaaaacga tcattgaact
      601 tcgatacgtt atccggcgtc ggtgctccaa aattcttctg cagaaatgcc tgaagctctg
      661 ccggatcgtt gatgattttt tccatctgtt ttgaattgta gaatttcggc tggatggcgg
      721 cggccttggc cagttccaac gtgagctgcg tcaccgtctt tgccatgggg tcgatggcaa
      781 acacactcac ttcactgctg cccttcttgc gggcataggc aaaataatga catacatcac
      841 ccgtgttctt ctccacaaag agaatggcat ttccgtccat tgtgggaaat tcggggttga
      901 ccacaccctg gatcatgtca aacaggatgg tcttacccga accgagtttt cccgcaatac
      961 aattcaggtt gtggttgaaa tgcaaactct ggcttccaag ggagggatga tcgaacacca
     1021 tgccatgcag ataaagacca tccaattgtt tgaaactatc ttcaattctc acccgcgagg
     1081 atggcatttt gatcgcggtc tgcaagcccg caaaggtggg ttccgtcatt ttgagatagg
     1141 tgaatcgttt cgcgatggaa gcgaggtcat gcgcatctga gccctgaaaa tatgccagat
     1201 acggaagccc ttctttacgc cgcatcgcgt caaactttgc cttgagtgag gtctttatct
     1261 ttttattggg ccggctgaac tttgccggat cgacaatttc cacagctctc aaatccttac
     1321 tttgcagaac gttttccatc gagtcgctgg tcagcttata tagatccaac agcgcctggt
     1381 cactgccatc gatgtgtgcc gggattgcca gtccgccttt ttggaggatg agttccagcg
     1441 cgtcatacac actggcgttg ccggcttccg ggttcttccc ccacatatcc acaccaaagc
     1501 caacttctgc caataaatcg cagatcatga aatgcacttt acgccgcgga ttctgcggtg
     1561 ggaagatggc aaggatgtgc acgcccgtgg tactgatctc cacacccggc agaaccaaca
     1621 gctctgtacg ccccacggca ttgttcacga tttgggcttg gtcgtcgatc aactcatacc
     1681 aggtcggcgc ggcgagatcc atgagacgct tctctgattc cttatccgcc cagatggtgc
     1741 cgatggcgtt atggtctgta acggcgacaa tggatagtcc cagctcgagg aaacgctgga
     1801 caatattgga tgcgatcaca cgcgcctctt tcaggatgcc cgcgtggatg gcgttcactt
     1861 gctcttcgta attgggattg ccggctcttt ttggatattg gatcttatag ggattgaagt
     1921 tataacgatt tcgcccgcgt gcatcctcgg aggcgggggt gtgaaaatgc aaatcggcct
     1981 tatagaacct tgtcccgtag ttcaagcctt ctactttttc aaacattttg agcagatcgc
     2041 ctggcatact cattgcgtcc tcctttgtgt cgatgaaaga cagcgtatcg cccgaatgta
     2101 atcagtatag cacgctgaag ctattttgct ccgtgcattt ggaaacaggg tgtgccaaaa
     2161 tcagaaacaa catgtcacct tccctcaggt agaccgaaac aacgcaagcc ttgatttctt
     2221 ttctattgct tcatgcgtac tcaagttatt gtattgctct tgctaacaaa ctgttataga
     2281 atactgagag atgaacaacg aagttaatca tgtaaagatc cctcgattac gtcgtcctga
     2341 gacgatccct ttgggattaa agattccagt tggtaagtct ttgcgttctg ccattcagaa
     2401 ggttgttgaa gaacttaaac ctgaaaagat cattcttttc ggctcttatg cgtatggaac
     2461 gccgagtccc catagtgatg tagatttgct ggtcattatg aaaacaaaag catcactcaa
     2521 agatagaagc tgggcggtat cacgtttgct attgccgcgt ccgtttccag tggatattct
     2581 agttaagact ccaaaagaaa taacgaaagc tcttgaatca ggagatttct ttctcaaaga
     2641 gatcatgact cgggggaagg ttttatatga ccgaaatcaa tgatcccatt gcctgggcag
     2701 aacgagcgga ggaagatttt attttagcgc gcacagcatt gagaagaaaa caacctctta
     2761 cgtacggcgc atgtttccat gctcagcaat gtgcggaaaa atatatgaag gctctgctga
     2821 tttccaaagg tgcggacttt cccaagacac atgatctact gatgttgaat gggctttgtt
     2881 cctctaacgg tatcttcctg gaaatcgact ctaaacactt gaacactcta actgattatg
     2941 ctgtccgtac gcggtatcct ggtaatgatc caacagtgga tgactcaaaa gaagcgctcg
     3001 aattagcaaa gttaattcga acctttgcca gaaaatatct tggtctataa ataaaacgcc
     3061 gagacacatt ctctcggcgt tttatttttc tacagcaatc gttttctgca gatgtatttg
     3121 tccaaataac gcggtcggta aaataacttt atcgaagcta ttgaaacgcg agcataattc
     3181 gactaagata tgatctatcc gcaagcatgg aacatcttca aattgcttat gacctgtttg
     3241 tgatcatgat cgggctggct gccctgtcga tcgtggtctt ttgggcagcg agaacggggg
     3301 aatctgacct gcggaatttc agcatcttat atacctgctt tactctggtc ctgatcgtgg
     3361 agctgttgga gaaatatctg ttcctgaacg tggaagacta ttccgcacgg ctatggtatg
     3421 cactctcggg aataggacag gggctcaaca ttgctgtcat tgtggcagcc atccacttct
     3481 ttgcgggagt ctatcaggtc cgctaccaaa gggcgatcac gatcttcttc ttagtggtga
     3541 tgcttgtgtc tctcgtgttg atcctctcgc cgatcggcgc cgtgctcaat gccgaggccc
     3601 ggagcatcag ctttaggatc gggttcaaga tcgcctccgt gttctacttc gtcgccttca
     3661 cctttatgat ggccctcggc tacggtctct tgcgccgcgc ctggcagacc gagaggcgga
     3721 acttcctgat cggcttattg ctctttgcca ccgtcggatt tattgaaact acgctcagcc
     3781 tccccgtggc gctgcgtagg gaatcggtca caatctcgac tggcagcaat tttctgtaca
     3841 gctccattcc ctacgcgttg tatgggatct tcctgatctt ttacttcctg cgttaccctg
     3901 ctcttgctcc gcttgagatg gacgatcttt cggcgacctt cctctcgaaa tacggcatca
     3961 ccgaccggga gcgcgagatc attctgaaag tagtccaggg gaaaagcaat gccgatatcg
     4021 ccagcgagtt attcatctct ttggcaacgg tcaagaccca tctgcacaat atctacagga
     4081 agatcgaagt ggatagccgc tacgacctgc tcgccagggt gaggtcgggt cagtaacgcc
     4141 aacccggtgg tcgagtagcc ccgaatgtgc tgagcgaaga cgaagcatga agcggcgtac
     4201 cgagaccacc aatttcatgg tttctttggg ctttttggtc tcgacacctg cctgatttgc
     4261 tgagcaaatc acgcatagtg ccagggagtt caggcgaggt cacgaaggaa acgagcccaa
     4321 aatttccttt cgtgtacgta gtgtccttcg tgtttaaggg ttttaggggc ggaattagcc
     4381 atctcaatcc aaagttgtag ataaaaattc actaaataca tccatcgctc gatgttcatt
     4441 tctcctcgcc tgcgcgaaga tgggcgaaat cccgaccggt cataccgccg gagacaaatc
     4501 ctaaggagaa tgaacatgaa agtgcaaacc accaatccag gaaaaccaac gataggcaag
     4561 gtgatcggtt ggacgatcat gctgctggca gccaccatgc cggtgctggt gagtcttggc
     4621 tatttgaccc tcgacccgga caactttccc tttacccaac aaaaggcagt ctacacggct
     4681 cacaagacga tgctgatcac gcacatcatt gcttccatga ttgccatcct gatcggcccg
     4741 ttccagtacc tgcccgggat ccgcaagggc cgtttcctca aagtgcatcg ctggctgggg
     4801 cgcacctacc tgctaagcgt cctgttcggc ggggtgagcg gcctgtatat ggcacagttc
     4861 gcccatggcg gaatcatcac cgaactgggc tttggggcat tggcagtact ctggctgtac
     4921 agcggctacc gcgcctaccg gcacatccgc aacaaggaca tcgagtctca tcgccagtgg
     4981 atgatccgca actacgcgct gacctttgca ggcgtcatgc tccgagtgtg gatgcctctc
     5041 tctgttagtc tgggagccga cttcacctca gcgtacatca tcgttgcctg ggcgtgctgg
     5101 gtccccaacc tgctggtcgc tgaatggatc attcgcggaa gccggcgcag ttcccggaga
     5161 gttggcccgt ccggtgctac ggtccgtgta caaaaaactg cacagatcaa ttctgaagaa
     5221 aatgtttatg cggattgacg aaaacagttt tgcctgaaaa agctaactca aatggattcc
     5281 aataaacatc tatcttgaac actaaaggaa aaagaaatga acactctact catgaagaag
     5341 tccctggcag catccatcac gatactgttg actctgggaa ttctcctatc gatcgtcgga
     5401 ccagtcaaag ctcataaaac cgcaaccgcg ctggattcaa atgtatccac gatgccatcc
     5461 caagagctgg gcgtgaccga ccctgctgaa cttgagaact ttcttgatgg tctattgact
     5521 aatgagatgg aagagaatca tatcgccgga gcagttattt cggtggtcaa agacggcaag
     5581 ttactttttg ccaaaggcta cggctacgct gacattgaaa acggcatccc ggtcgacccc
     5641 gaacagacgg tctttcgcat tggctcagtt accaagctgt tcacctggac ggcagtgatg
     5701 cagctcgcgg agcagggcaa gctggacctg gacgcggata tcaataccta tctcgacttt
     5761 cgcatcccgg acacttatcc acaaccgatc accctcacgc acctgctgac ccatacgcca
     5821 ggatttgaag accggttttt cgaggtcctg gcatcagatg tcaatgatct ggttcctgtg
     5881 cgcgaatggc tggtctccca tatgcccgcg cgggtgcgtc cgcctggcc
//
LOCUS       DASTJP010000279         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1454783, whole genome shotgun sequence.
ACCESSION   DASTJP010000279 DASTJP010000000
VERSION     DASTJP010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454783"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VFQ23_08385"
     CDS             <1..1666
                     /locus_tag="VFQ23_08385"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906645.1"
                     /translation="VIYQEQSHWATALDCFQRARSLAQSVGDIAGEVLHTSQMAAVYR
                     QQGNWNQAASLLEEALGVYRQQGDLKGEADCLGDQATLFHLRGDLSTALSLNRQALEL
                     YEKIGHRLGQASTLGNIGLIQQDRGDLNEAESAFLASLDLEEKAGHISGMAASHGNLG
                     LLYGLRGNLEAAETHSQKALVLMRQIGDLSGEAKVLGNLGTLAYDRGNFAAALTFHQE
                     AQQIDAQIGDRLDEAADWANIALVYRVMGALQNSLDAHQQALRLNRGLGYLEGLANTL
                     TNIAALHRQLGKPGEAEHCYHQALELYQRCRMPIGEIGVWNGLGHLYYLSNDLSEAAN
                     CYQQALSLSQAGNFRQNQVDSLGNLGVISQARHEWDQAEFDFQQALALARAVHYIAGE
                     IAVLVNLGYTYLMAGKPELALLTQNEAVAQAHTHQNPDLLLRALAGRGDTHCKIGNFT
                     QAKSDYSEAVQIVETLRKSLEQELDRITYFGAGKPVLYQRIISVLVRGLYDRASAFDY
                     TERSRSRTLLDLLTQSKSTLTLPIATNDFPLNHAEIRGLLTTNVNR"
     gene            1827..>2619
                     /locus_tag="VFQ23_08390"
     CDS             1827..>2619
                     /locus_tag="VFQ23_08390"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HET9906646.1"
                     /translation="MDTSISFSQVVPLQCPECGVPITAEVWWIIDESERPDLIERCRR
                     GDIHDLACPNGHVGMAKAPLLLRLTSRERILFSPPESTTREQDDAMKLALAQRLQSAL
                     SEISLEQLLTQIEQTSRLFLPLVLKGKPDDQIGALKALLRELYQVVPSTETPRRIGII
                     HQALKQISREDSPPLWALLQAELGFTYLDSTTGDRAENLEQAIVAFHSALSVWSRTTY
                     PDAWAEAQNNLGEAYRNRVLGDRSLNIESAINAYLNALIVRTRDVN"
BASE COUNT          655 a          637 c          678 g          649 t
ORIGIN      
        1 cgttatctac caggaacaaa gccattgggc aacggccttg gactgctttc agcgagcacg
       61 atcgctggca cagagtgttg gtgacatagc tggagaggtt ttgcacacca gccagatggc
      121 tgcggtgtat cgccagcagg gcaactggaa tcaggctgcg tcgctgttgg aagaggcact
      181 gggagtatat cgacagcaag gggaccttaa gggagaagcc gactgcctcg gtgatcaagc
      241 tactcttttc catcttcgcg gcgatttgtc tactgccctt agcttgaaca ggcaggcgct
      301 tgaactttac gaaaagattg gtcatcgact tggtcaagca tcgactctag gcaacattgg
      361 attgattcaa caggataggg gcgatttgaa tgaggctgag tctgcatttc tggcgtcttt
      421 agatttagaa gaaaaggctg gtcacatctc gggtatggca gccagccacg gtaatcttgg
      481 cttactctat gggctccgtg gcaatcttga agccgctgaa acccatagcc agaaggccct
      541 agtgttgatg cgacagattg gggacctttc cggcgaggca aaggtcctgg gcaatctcgg
      601 tactcttgct tatgaccgag gcaatttcgc ggcagcgctt accttccatc aggaagctca
      661 gcaaattgac gcgcaaatcg gagatcgatt ggacgaggct gctgattggg caaatatagc
      721 ccttgtttat cgggtcatgg gcgcgctaca aaattcgttg gatgctcatc aacaggcttt
      781 gcgcctaaat cggggacttg gttatttgga aggtcttgca aacactctta ccaacattgc
      841 agcactacat cgccaattgg ggaaacctgg tgaagcggaa cattgttatc atcaggctct
      901 agagctttat caacggtgta gaatgcccat aggcgagata ggggtctgga atggattggg
      961 gcacctatat tacttatcaa acgatctgag tgaagccgcg aattgttatc aacaagcgtt
     1021 gtcccttagc caggccggca attttcgaca aaaccaagtt gatagcttgg ggaatttggg
     1081 tgtgatctct caagcccggc atgaatggga ccaagccgaa ttcgattttc aacaagcgct
     1141 tgctttggca agagcagtcc attatatagc tggtgaaatc gctgtgctgg tcaatttagg
     1201 ttatacctac ctgatggccg gaaaaccaga actggctttg ctgacgcaaa acgaagccgt
     1261 tgcccaagct cacacccatc agaatccaga cttattgttg cgagctttgg ccggacgagg
     1321 ggatacccat tgcaagattg gcaattttac gcaagccaaa agcgattact ccgaggcagt
     1381 gcagattgtt gaaactcttc ggaaaagtct tgagcaagag ctggatcgga ttacgtattt
     1441 tggggccggt aaacctgtgc tatatcagcg aatcatatct gtgttggtcc gaggcctata
     1501 tgacagagcc agcgcctttg attacactga acgcagtcgc tcgcgtactc tattggatct
     1561 actaactcag agtaagagca ctcttacact gcccattgct accaacgatt ttccgctgaa
     1621 ccatgcagaa atacgcggtc ttctcactac aaatgtaaat cgctgaacag taactctata
     1681 gctgaatttc atctccagat tggagatata gtggttcaca gtgattgtcc ttgtttgctg
     1741 tggtgatcat attaagtaga aacgcatacg cgccggaggc ctgtttacgc ttggcacaac
     1801 tagcaaaaca cgctttcaga aattagatgg atacctcaat atccttttct caagttgttc
     1861 ctttgcaatg ccccgaatgc ggcgtaccaa ttaccgcaga agtctggtgg attatcgatg
     1921 agtccgagcg cccggacctc atcgaacgtt gtcgtcgagg tgacatccat gacctagcat
     1981 gtcccaatgg acacgtaggc atggcgaaag cgccgctgct actccgactc acgagtcgtg
     2041 agcgaatttt gttctctcca ccagagagca ccacacgaga gcaggatgat gcgatgaagc
     2101 ttgcgctggc acagcggctt cagtctgcct taagtgaaat cagccttgaa cagttgctaa
     2161 ctcagattga gcaaacctcg cggctcttcc tacctttggt gttgaaggga aaacctgatg
     2221 accaaatcgg cgccctcaag gctcttttac gagagctata tcaggtcgtt ccgtccaccg
     2281 agacgccacg ccgcatcggc attatccatc aggcattgaa gcaaatcagc cgtgaggata
     2341 gcccgccgct ctgggctctg cttcaagcag agctgggatt tacctatttg gattcaacca
     2401 ctggcgatcg tgctgagaat ttagagcagg caattgtggc ttttcattcc gccctgtctg
     2461 tatggtcgcg gacgacctat cccgatgcat gggctgaagc gcaaaataac ctgggtgaag
     2521 cttaccgcaa tcgcgtgctc ggtgacagaa gtcttaacat cgaatctgca atcaacgcct
     2581 atctgaacgc gcttattgtg cgcactcgag atgttaacc
//
LOCUS       DASTJP010000280        16355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_251041, whole genome shotgun sequence.
ACCESSION   DASTJP010000280 DASTJP010000000
VERSION     DASTJP010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251041"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFQ23_08395"
     CDS             <1..1033
                     /locus_tag="VFQ23_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET9906647.1"
                     /translation="KLIGREYFILEGTEDEADSEIMEQFVTQFYTEAATVPPQVMLPE
                     EIEEARIISQWLRSRRGGEKVEFFVPKEGQPQELVQLAAENAKETLQALRAQWQADTH
                     KQEQALAELHQALNLPEPPNRIECYDISNMQGTAIVGAMVVFTQGVADKKLYRKFNIE
                     SVVGAPDDFASMEEMLTRRFRRWRSSQEKADEPGAKKDESFSFLPDLIIIDGGKGQLG
                     RAVQVLENFELLDKIPVIGLAKQQEEIFFPHKSDSLMLPRHSQGLYLIQRIRDEAHRY
                     GITAHRKRRQKIGMASQLDSIPGIGPARRKALLKHFGSIDKIREASIQDLRSVVPEDA
                     AQSIKSHLE"
     gene            1036..1425
                     /locus_tag="VFQ23_08400"
     CDS             1036..1425
                     /locus_tag="VFQ23_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9906648.1"
                     /translation="MPKHIWVIDDDEEMNRAVGLMLKLLNYEVASFFNVRAAAQTLLI
                     GRRPDLFILDLNLPEVTGLDMIEFLRRRPESKNIPIVMLSSEAAGSVLDKALELGADA
                     YIKKPVIIEELETAMTTAFEKHTLPEK"
     gene            1760..2836
                     /gene="prfA"
                     /locus_tag="VFQ23_08405"
     CDS             1760..2836
                     /gene="prfA"
                     /locus_tag="VFQ23_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET9906649.1"
                     /translation="MLDKLKGIEDRYQQLGNELLDVGSDYQRAAEINKERVDLESIVE
                     KAREYRQAMKSLEEAKSILESEKDSELLALAESDIADLTPKTETLEKEIKGMLVPKDP
                     RDDKNVIVEVRAGTGGDEAAIFAADLFRMYTRYAEGQRWKNEILSENAIGVGGFKEVI
                     FEIRGKGAYSKLKYESGVHRVQRVPATESQGRIHTSTATVAVLAEVDEVEIDIPESDI
                     EVEVYRSSGAGGQNVQKNSTAVRLTHKPTGMVVTCQDERSQLQNKLRALSILRARLFE
                     IEQEKRRSEIESDRRSQVGTGERSEKIRTYNYPQNRVTDHRIGLSNYNLPAVMDGGID
                     EFIEELSTRDEADRLAASGVDDEE"
     gene            3007..3945
                     /gene="speB"
                     /locus_tag="VFQ23_08410"
     CDS             3007..3945
                     /gene="speB"
                     /locus_tag="VFQ23_08410"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF002564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET9906650.1"
                     /translation="MNDNPAFGAGLQPFMGIPSFMRIPVTRELTDVDVAILGIPFDSG
                     TSYRTGTRFGPRKIREASLLIWGHNSTLNVSPLKKLNIVDYGDVSVIPTSIEHTMTAI
                     TNTAAEIIDTGTTLISLGGDHSIALPLLRAHAKKHGPVSLVHIDAHLDTWESEFESVP
                     YSHGTPFRYALQEGLLRKGEYIQIGIRGPLSGENDYAEAAELGARTITIHEVMEKGIS
                     EILKEVHERMKGPVYVTVDIDSADPAFAPGTGTPEVGGLTSYQLLQLVRGLHGLNLIG
                     FDLVEVSPPFDHGDITAILASNIVFEYLSLLAIKNG"
     gene            3938..4813
                     /gene="prmC"
                     /locus_tag="VFQ23_08415"
     CDS             3938..4813
                     /gene="prmC"
                     /locus_tag="VFQ23_08415"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET9906651.1"
                     /translation="MDKASSLHSEITTRLTTISDTPSLDASVLIAHVIQKPRSWVMAH
                     PEARLTNTEQKQLSDSLVRLKGGEPLPYVLGHWEFFGLEFDVTPDVLIPRPETELLVE
                     KAISWLKRLSLPDPRVADVGTGSGAIAISLAVHLPEVQILATDISGKALNVAKRNARK
                     FKVEDRIEFVECDLLPETSAFKNRQSAFNMICANLPYIPTDELHRLPIYEREPTLALD
                     GGADGLDQIRKLIRLAPTCLAPNGLILLEIEASQGAMALSLARAAFPNSSIELHRDLA
                     GKDRLLGINFSRINI"
     gene            4921..7002
                     /locus_tag="VFQ23_08420"
     CDS             4921..7002
                     /locus_tag="VFQ23_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9906652.1"
                     /translation="MKQLRASMNRPVFEDEDQTRIAGFLYTIILTLGAGTVLYILVTQ
                     AFTVRITRSLNFTLLTAVLFLLSLYILTWRGYLRIVSILLIAGLWSLQTGAIYFDGGV
                     SSPVFAGYAIIIIIASVIFGGQAGLLIAIISMLTGAGFVIADNRGLLPAASNSALNEW
                     ASHSLFFVFTATLLALFSRDLKSALENARRSAQTIHASEEALRESEVKYRTLVERIPA
                     IIYTAAMDQSKTRIYVSPQVERILGFTQEEYLANPELWRELLHPDDRQRIIGEAEYFY
                     ATGEPFISEYRTFTRDGRVIWLHDEAVILRDSAGKPQWIQGVRMDITELKQVEEKIKT
                     PLAQLASLRAIDVAIMSTHDLRMIFYILLSQTIAKLKVDAARILLFNPSERILEFGAA
                     QGFLTPDVESVHIHLGEEIAGRAASEGQLIDGHRLTREFFASNAPHLIDEGFSCFYAV
                     PLISKGKIKGVLEVFEREHHSHNRDWVDFLNALAGQAAIAIDNVSLFRELQRSNSDLS
                     LAYESTLEGWSAALDLRDRETEGHTQRVTALSEQIARSMGITGNELLNIRRGALLHDI
                     GKVGVPDRILHKRGPLTKNEWKIMQKHPQFAFDMLSPIAYLHGALDIPFCHHEKWDGT
                     GYPRGLKGEEIPLAARIFAVVDVYDALISNRPYRKGWSRKKALKYIREQSGIHFEPQI
                     VQAFLREMEKK"
     gene            7002..7628
                     /locus_tag="VFQ23_08425"
     CDS             7002..7628
                     /locus_tag="VFQ23_08425"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET9906653.1"
                     /translation="MPMQTEILSANHHEAIPRALEVLHQGGLVAFPTDTVYGLGSLAF
                     DGKAVESIYIAKDRPIEKAIPVLIGDDDDLEKVSNGVTEVVRRLATQFWPGPLTIIVP
                     KVPALPEAVSATNTVGVRIPDHPVARALLRSTGPLAVTSANISGQPSPSTAQDVFEQL
                     GGRIELIIDGGTTPGGVPSTLVDCTGSEIRVLREGPISLEQILSAISQ"
     gene            complement(7777..8805)
                     /gene="tsaD"
                     /locus_tag="VFQ23_08430"
     CDS             complement(7777..8805)
                     /gene="tsaD"
                     /locus_tag="VFQ23_08430"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET9906654.1"
                     /translation="MSNQKLTRILAIETSCDETACAVIENGRALLASTVASQMDIHAR
                     YGGVFPEVASRQHVLSIIPVVEQTLAQSNLTLKDIDALAATRGPGLAGSLVVGMNMAK
                     GLALGTGLPLIGVNHLEGHIYSSWIYKASENVPDEPKFPLMALLVSGGHTELNLMTDH
                     LTYQRLGSTLDDAAGEAFDKVARLLGLPFPGGPSIQKASETGDPTRFKFPRARLDNPY
                     DFSFSGLKTAVLYEVQNLKKNGNALPVEDLAASFQATVVDILFNKTIKAARDLGAREI
                     LVAGGVSANRPLREAFQAQSEFKVHIPPFSLCTDNAAMIAAAGHYRFISGHFSELDID
                     VQPTWPLS"
     gene            complement(8909..10129)
                     /locus_tag="VFQ23_08435"
     CDS             complement(8909..10129)
                     /locus_tag="VFQ23_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET9906655.1"
                     /translation="MKSDLDELMKLRNLDALMILGNAEHNPPMYYLTGGGHVSKAVLI
                     KKIGQPAVLYVNAMEREEAERSGLKVVPTRQAAMTELSQRPKEIFGEHDLVSGRVGVY
                     GETNVSMVLHIENRIKDELPDIELIGEDQGDSLFMYAMEAKDESEAARIRSMGKATTL
                     VVDKVREYLTGCDVRDDEVLLKEDGTPLTVGEVHSKIRLWVSEQGAELPSGFIFATGR
                     DAGVPHSAGTASELMKLGQTIVFDIYPAEAGGGYYYDFTRTWSLGYATPEAQQLYDEV
                     KDVYDKVIENIDLNVSFKEYNRMVCDEFGKNGHKTPLNMDGVVTDGYVHSLGHGVGLN
                     IHERPWSSPSASDNNLLKPGVVITVEPGLYYPEKGMGVRIEDSYWIRPDGTMEKLAEY
                     PYDFVLPMKKWKKG"
     gene            complement(10126..10626)
                     /locus_tag="VFQ23_08440"
     CDS             complement(10126..10626)
                     /locus_tag="VFQ23_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906656.1"
                     /translation="MRIWTLKLTTGEQKRGQMVELALPVPLCVDCAAKEDKIANITWL
                     PFFISGLLVCVAAFIPVWLITPDGPTSQPESLPLLLGAFVGLIAGILGGTIVELLLKL
                     MFAPAYGQLLWKRPLTAFMVFANVEQIMGFTAKLSKNKQTLHVTIENDEVANEFKKLN
                     IESGVV"
     gene            complement(10709..12055)
                     /gene="selA"
                     /locus_tag="VFQ23_08445"
     CDS             complement(10709..12055)
                     /gene="selA"
                     /locus_tag="VFQ23_08445"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET9906657.1"
                     /translation="MINLRDLPSVDVLLQSAGHLIETYGRPLTLDSIRITLDETRSQF
                     KQDAEAVLPSNDVILARAEAHLSTWTSATLQPIINATGVILHTNLGRAPLSEESIRAM
                     EEASRNYSNLEYDLTKGQRGSRLVHAESVLKKLTGAEAALVVNNNASAVLLVLSALAN
                     KKRVIISRTQLVEIGGGFRVPDVMKGSGAKLVEVGTTNKVRLSDYEEALEDPAALVMR
                     AHRSNFKIVGFTEEPDLKDMVSVAHKANIPLLDDLGSGALLDTSTYGLAHEPTVQESL
                     AAGVDLVCFSGDKLLGGPQAGIIVGKADLIAKIKKHPLARAVRADKTCLAGITATLFH
                     YLKDEAERKIPIWRMISLTPEKVRVRAEAWQAELGQGEVIKSESTVGGGSLPEESLPT
                     YVLSLTVKGPDKFLKKLREGNPPVIARVENQAVLLDPRTVLQDEALLNALKVALRG"
     gene            complement(12039..12452)
                     /locus_tag="VFQ23_08450"
     CDS             complement(12039..12452)
                     /locus_tag="VFQ23_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906658.1"
                     /translation="MESPHNFKSFALASLLLFVIGWGGLWTIVNLAFPFVWYRWGFFI
                     LLIAAITGSMLPIVYFFHRRFPGGPPAEANVIIRQAMWFGIYGATLAWLQLGRLVTVY
                     VILGLAGGLIAIEYFIRLREKAHWKPPITSDDQSS"
     gene            complement(12546..13325)
                     /locus_tag="VFQ23_08455"
     CDS             complement(12546..13325)
                     /locus_tag="VFQ23_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906659.1"
                     /translation="MQLNSQRRLGLVLGLVLGLGFSLTSNLINQFFLPNLPLFVPWPG
                     TVGVILITTLMFGALGLIAAWPEEALPGIIASALVGSFVSALWIIINETGNRTGALIA
                     LFFVFLPRVFFYLPFGWLIRWLISRLEVSAYRQVAPARRLLTVVIAFALAVLAGTFSR
                     YPQEVRQSLARMEDLLQEGMQASSRDELPRPLQSVEGFVANADGPYTYTIGSDPDALP
                     VQRPVVNYGELEPFIIIRFENGFRFGCVFSPPYVVPACIDY"
     gene            13480..14028
                     /locus_tag="VFQ23_08460"
     CDS             13480..14028
                     /locus_tag="VFQ23_08460"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9906660.1"
                     /translation="MNVSDAIRLKRAVRKFQEKSLPEDVIRAILNAGRRSQSSKNEQG
                     WQFIAIRDKAILKALSECGTYAGHLAGAALGVAILTPNPEGKFQDLFDAGQTAAYMQL
                     MALELGVGSCLASIYEPAKAREILGFPLDWHLRIAISFGYPADAEKLTASPKKGGRAP
                     LEDLVHWDQWQDEIRDNQRSDG"
     gene            14021..14284
                     /locus_tag="VFQ23_08465"
     CDS             14021..14284
                     /locus_tag="VFQ23_08465"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET9906661.1"
                     /translation="MDEMKFTQLVEVMGRWKAEILEGLLKNEGIDVVLIQDSISSSTY
                     ANPFAPVQVFVPNEALSQAQELLEDFETTEETMEDEDESGDEE"
     gene            14378..14593
                     /locus_tag="VFQ23_08470"
     CDS             14378..14593
                     /locus_tag="VFQ23_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET9906662.1"
                     /translation="MPDSVYKVIELVGTSTESWERAATVAVERASQSLRDLRIAEVVQ
                     LDMVIDEGKVTAYRAKVKVSFKYESGG"
     gene            14757..15044
                     /locus_tag="VFQ23_08475"
     CDS             14757..15044
                     /locus_tag="VFQ23_08475"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET9906663.1"
                     /translation="MTAYVIVDIQVTDPVGYEEYKKLATSTVEQYGGKYIARGGKTEI
                     LEGEWSPGRLVILEFENSEQAKKWLNSPEYNHPRQLRHKTATSNMVVIEGV"
     gene            15058..15870
                     /locus_tag="VFQ23_08480"
     CDS             15058..15870
                     /locus_tag="VFQ23_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9906664.1"
                     /translation="MFELESYIKQFADKTIAIAAYDLEAGTEILINADESFHPASTIK
                     VHVMMEVFRQAEAGLLSLDDCIPVINSFTSIADGSKFGLDVKDDSETTLYQRLGESES
                     ILELTLLMIVRSSNLATNILLEKVGAKSVQDFVHELGIDGVIVKRGVEDNVAFRLGMN
                     NSATARGLTQTMQLLAEGKVVSEKASAQMIEIMLQQEFNESIPALLPGAAKVAHKTGW
                     TSEFYHDTGIVFPENRKPYAISLMTRGFAEANEAQAHDCMANISRIIYEKLQ"
     gene            15867..>16355
                     /locus_tag="VFQ23_08485"
     CDS             15867..>16355
                     /locus_tag="VFQ23_08485"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HET9906665.1"
                     /translation="MITISSLTYYPIKACRGFEIESANIERMGLQNDRRMMVVSPEGG
                     HLTQREHPRLALVTPKLENGTVTLSAPNYDSLQFATQTTGATYPVSIWKSKGVQAIDQ
                     GDDAAQWFSDWLGTPVRLVHIAGGLQRRVDGEYAVNADDHTGFADGYPILVTSESGLQ
                     DLN"
BASE COUNT         4303 a         4024 c         4109 g         3919 t
ORIGIN      
        1 gaaattgatc ggacgcgaat actttattct tgaaggtacc gaagacgaag ccgatagcga
       61 gatcatggag cagttcgtga ctcagtttta cacagaggct gccacggtgc ctccacaggt
      121 aatgctgcct gaagaaatcg aggaagcgcg cattatcagc cagtggctac gctctcggcg
      181 cggtggtgaa aaagtggaat tctttgttcc aaaagaggga cagccgcagg aattggtcca
      241 attggcagcg gaaaacgcca aagagaccct acaagcgctg cgtgcacaat ggcaggcgga
      301 tacgcataaa caggaacaag ccctggcaga attacaccag gcactgaatt tgccagagcc
      361 tcccaatcga atcgaatgct acgatatttc gaatatgcag ggtacggcaa ttgtcggagc
      421 gatggtcgtc ttcacacagg gcgtggcaga caaaaagtta tatcgcaagt ttaatatcga
      481 aagtgtggtg ggtgcgccag acgatttcgc gtccatggag gaaatgctga cgcgcagatt
      541 ccgtcgctgg cgctcgtctc aggaaaaggc cgatgaaccc ggggccaaaa aggatgaatc
      601 gttttctttt cttccagact tgattattat cgatggtggt aagggtcaac tgggacgcgc
      661 cgtccaggtt ctggagaatt ttgagttgct ggataagata ccggtgattg ggcttgccaa
      721 acagcaggaa gaaatcttct tcccccacaa atctgactct ttgatgctgc cgcgacattc
      781 acagggcttg tatctaatcc agcgaattcg tgatgaagcc catcgctatg ggatcaccgc
      841 ccatcgaaag cgcagacaaa aaatcggcat ggcttcgcaa ttggactcca ttccgggaat
      901 aggacccgcg cgacgcaagg cactcttaaa acattttgga tccatcgata aaatacgaga
      961 agcgtcgatt caggatttgc gctcggtagt tccggaggat gcggcgcaat cgataaagtc
     1021 tcacctggaa taagtatgcc gaagcacatt tgggttattg atgacgatga ggagatgaac
     1081 cgtgcagttg gattgatgtt aaaattgctg aattatgagg tcgcttcgtt tttcaacgtg
     1141 cgcgcagccg cccagacgtt gctgattggc cgacgacccg acttgttcat tttggatctc
     1201 aatctgcccg aggttacagg attggacatg attgagtttt tgcggaggcg gcccgaatcg
     1261 aaaaacatac cgattgttat gctctcgtcg gaggcagccg gcagcgtctt agacaaagcg
     1321 cttgaactag gcgctgacgc ttacatcaag aagccagtca tcatcgaaga acttgaaacc
     1381 gccatgacaa cggcttttga gaaacacact ttacctgaga aataaaatgg caaacagtaa
     1441 atcaaaaagc aagaatcaaa caaaaccaaa ggaaactaag ccagccagca acaggcttgt
     1501 acaaatcgta tttatcgttt tctccatcat cctaatcctt tcaatggttt tgtccctcac
     1561 atcaactttc tagcgcttca ccacaggatg atataatttt tctgcgcggt agattttgcc
     1621 gcgcatttat tacataaatt tcattgcgag gagaagcgac acttgcacct ggcgcacgcc
     1681 gccctggttg gtcggtgcga tttttgaaat gctttagcat ttcaaaaatc gggagttcag
     1741 gtgtaatctg aggataggta tgctcgataa actaaaaggc attgaagacc gctatcaaca
     1801 gcttggaaat gaactgctcg atgtgggcag tgactatcaa cgcgccgcag agatcaacaa
     1861 ggaacgcgtg gatctggaat ccattgtgga aaaggcccgc gaataccgtc aggccatgaa
     1921 gagcctggag gaagccaaat caatccttga gtctgaaaag gattctgaac tgctggctct
     1981 agcagaatca gatatcgccg atctgacgcc gaaaactgaa acccttgaaa aagagatcaa
     2041 gggcatgctg gtgccgaaag atccgcgcga tgacaagaac gtcattgtcg aggtgcgcgc
     2101 cgggactgga ggtgacgagg cagctatctt cgccgcggat ctgttccgca tgtacactcg
     2161 ctacgcagaa ggccaacgat ggaaaaatga aatcctttca gagaacgcaa ttggcgttgg
     2221 cggctttaaa gaggtcatct ttgaaatccg aggcaaggga gcctattcga aacttaaata
     2281 cgaatcaggc gtccatcgtg tccagcgagt tccagcgacg gaatcacagg gacgcattca
     2341 cacgtcgacc gcaaccgtcg ccgtgcttgc cgaagtggat gaggtggaga tcgatattcc
     2401 tgaaagtgat atcgaggtgg aagtatatcg ctcgtctggc gccggcgggc agaacgtaca
     2461 gaaaaactct acggctgtgc gtctcacaca caagcctact ggcatggtcg tgacctgtca
     2521 ggatgagcgt tcccagttac aaaacaaact gcgcgcgctg agcattctgc gcgcccgact
     2581 gttcgagatt gaacaagaaa agcgccgctc tgagatcgaa tctgaccgtc ggtctcaggt
     2641 gggaaccggt gaacgttccg agaagattcg cacctacaac tatccacaaa accgcgtgac
     2701 agaccatcgc attggtttat cgaactacaa cctgccggca gtgatggatg gcgggatcga
     2761 tgaattcatc gaagaacttt caacgagaga tgaagcggat cggctggctg ccagcggcgt
     2821 tgatgacgaa gaataattgt tcggttggtc cagtagtcct gcatactttt cgcgagacgg
     2881 atcgagacca acacgcatat gaaaatttag tattatttga aaacttggtg gtttcgatat
     2941 gaccttcgca acaaacgctc aggtctactc aaccaccagt tttattatgg ataaagggag
     3001 ataaaaatga acgacaaccc cgcatttggc gcaggcctgc agccttttat gggcattccg
     3061 tcgttcatgc gaattcctgt tacacgtgag ttgacggatg tagatgttgc catcctggga
     3121 ataccgtttg acagcgggac gtcgtaccga accggcacgc gatttggtcc cagaaaaatt
     3181 cgcgaagctt ctttgctgat ctgggggcac aactcgacgt tgaacgtgag ccccttgaag
     3241 aaactcaaca ttgttgacta tggcgatgtg tctgtgattc caacgtcgat cgaacacacg
     3301 atgacagcca taacgaatac agccgctgaa attattgata ccggcacaac ccttatttcg
     3361 ctgggtggcg accattcgat tgcgcttccc ctcttacgcg cacatgccaa aaaacacgga
     3421 cctgtttctc tcgttcatat cgatgcacat ttggatacct gggaatcaga atttgagtcc
     3481 gtcccataca gtcatggaac accatttcga tatgccctac aggaaggcct actccgtaaa
     3541 ggtgaatata tccaaattgg gattcgtggt ccgctcagtg gtgaaaatga ttatgccgag
     3601 gctgccgagt tgggggcccg aacgatcaca atccatgagg taatggaaaa gggcatcagt
     3661 gaaattctca aggaagttca cgaacgcatg aagggaccgg tttacgtcac cgtggatatt
     3721 gattcagccg atcccgcgtt cgctccggga accggaactc ctgaagtcgg tgggctgacg
     3781 agttatcaat tgctgcagct tgtgcgcggc ttacatggac taaatctaat cggcttcgat
     3841 ttagtagagg tatcccctcc atttgatcat ggggatatca cagcgatcct tgcatccaac
     3901 attgtatttg aatatttatc cctgctggcg atcaagaatg gataaagcat cgtcgcttca
     3961 ttcagaaatt accacccgcc tcaccacaat tagcgatacc ccttccctgg acgcatccgt
     4021 cttaatagcg cacgtgatcc aaaaaccgcg tagctgggtg atggcacatc cggaagccag
     4081 actcacaaac acagaacaaa aacaactgtc cgactctctg gtccggttga aaggtggtga
     4141 gcctctgccg tatgtgctcg gtcattggga attctttggc ctggagtttg acgtaacgcc
     4201 agatgtatta atccctcgtc ctgaaacaga gttgctggtt gaaaaagcga tatcgtggtt
     4261 aaaaaggctt tcactacccg acccgagagt tgcagatgtt ggcacgggct cgggcgcaat
     4321 tgcgatttct cttgccgttc atctaccaga ggtgcagatc cttgcgacag atatttccgg
     4381 aaaagccttg aatgtcgcaa aacgcaatgc acgaaaattc aaggtggaag atcgaatcga
     4441 atttgttgaa tgcgatttgc tccccgaaac atcggctttc aaaaatcgac agtctgcgtt
     4501 caatatgatt tgtgccaacc tcccctacat ccccacggat gaattacaca ggctgccaat
     4561 ttatgaacgc gaacctactc tagcactgga tggcggtgcg gatggtcttg accaaatccg
     4621 aaagctgatc aggcttgcgc cgacctgcct cgccccgaat ggattgattt tgcttgagat
     4681 cgaagcctca cagggagcga tggcactttc tctggcaaga gccgcattcc cgaattccag
     4741 cattgaacta cacagggacc tggcggggaa agatcgcctc ctcggcataa atttctctcg
     4801 aattaatata tgattgatag gcaactttaa tcaaccgtgg gtacaatcca ggggtggcaa
     4861 atttatctcg aaaaactccc aacgacgtaa cgaacgtaga ataagcaaaa tcgcattcta
     4921 atgaaacagc ttcgcgcatc catgaaccga cccgtgtttg aggatgagga tcagactcgt
     4981 attgcagggt tcctttacac aatcatcctg accctgggag cagggacggt tttatatatc
     5041 cttgtcactc aggcctttac tgtccgaata actcggtcgc tcaactttac cctcttaact
     5101 gcagtcttat tcttattgtc actttatatt cttacgtggc gcggatatct gcgcatcgta
     5161 agcattttgc tcattgcagg gttatggtcg ctccagacag gggccattta tttcgatggc
     5221 ggtgtgtctt cgccggtttt tgcaggatat gcaatcatta tcattattgc aagtgtgatc
     5281 ttcggcggac aagccggtct gctcattgct atcatcagca tgctcacagg tgcagggttt
     5341 gtaattgctg acaacagagg gttacttcca gctgcatcca actcagctct aaatgaatgg
     5401 gcatctcact cgttgttctt tgtttttacg gcgactttac tggcgctctt tagcagagat
     5461 ctaaagtctg cgttggagaa tgctcgtcgc tcggcacaaa cgatccatgc cagcgaggag
     5521 gcactgcgcg agagcgaagt gaagtaccgc acacttgtgg agcgtatccc ggccatcatc
     5581 tacactgccg cgatggatca gtccaagaca cgtatttatg tgagcccaca ggtcgaacgg
     5641 attctcggtt tcacccagga ggaatatctg gcaaatcctg aactttggcg tgagctgctt
     5701 catccggatg accgccaacg gattatcggc gaggccgaat atttttatgc aacgggcgaa
     5761 cctttcatct cggagtatcg cacattcacg cgcgatggac gcgttatctg gcttcacgat
     5821 gaagctgtga ttttgcgaga cagcgccggc aaacctcaat ggattcaagg cgtgcggatg
     5881 gatattacag aactcaagca ggtcgaggaa aaaataaaaa cgccgctggc acagttagcc
     5941 tccttgcgcg ccattgatgt cgccatcatg tccacccatg acctgcggat gattttttat
     6001 attcttctga gtcagaccat cgcaaaattg aaagtggatg cagctcggat cctattattc
     6061 aatccatcag aaagaatact ggaatttggg gctgcgcagg gatttcttac accggatgta
     6121 gaatcagtcc acattcatct gggtgaagag attgctggcc gcgccgcttc tgagggccag
     6181 ctcatcgatg ggcatcggct gacacgcgaa tttttcgcat cgaatgctcc ccaccttatc
     6241 gacgaaggct ttagttgctt ttacgctgtg cctttgatca gtaaaggaaa aataaaaggg
     6301 gtcctcgaag tgtttgagcg ggaacatcac tcccataaca gggactgggt ggattttctt
     6361 aatgctctcg ccgggcaagc ggcgatcgcg attgataatg tgtcgctttt ccgtgaacta
     6421 cagcgttcga actccgatct cagccttgct tacgagagta cgctggaggg ctggtccgct
     6481 gcactcgacc ttcgcgatcg agaaacggaa ggtcatacgc aacgtgtaac agcgcttagc
     6541 gaacaaatcg cgaggagtat gggaattaca ggaaacgagc ttctcaacat cagacgaggg
     6601 gccctgctgc acgatatagg caaagtaggt gtccctgacc ggattttgca caagcgtggg
     6661 ccgctgacaa agaatgagtg gaagatcatg cagaaacatc cgcaatttgc attcgatatg
     6721 ctttcgccaa ttgcatatct acacggcgcc ctggatatcc ctttttgtca ccatgaaaaa
     6781 tgggatggga ccggctatcc ccggggactg aaaggtgagg aaattccact ggcagcacgt
     6841 atctttgctg ttgtcgacgt ctatgacgcg ctgatttcta atcgccccta tcgcaaaggt
     6901 tggtcacgaa aaaaagcact aaaatatatt cgcgaacagt ctgggattca ttttgagcca
     6961 cagatcgtac aagcattcct acgcgagatg gaaaagaagt aatgccgatg caaacagaaa
     7021 tcctttcagc caatcatcac gaagcgatac cgcgcgcttt ggaagttttg caccagggcg
     7081 gcttggttgc cttccctacc gataccgttt atggattagg ttcgttagcc tttgacggaa
     7141 aagcagttga atcaatctac attgcgaaag acaggcccat cgaaaaggcg attcctgttt
     7201 tgatcggaga cgatgacgat ttggagaaag tatcaaatgg tgtcacagag gtagttcgca
     7261 ggcttgccac ccagttctgg cccggacctc tgaccattat cgtccctaaa gtgccggctc
     7321 tacccgaagc agtctctgcg acgaatactg ttggggtgcg gattccggat caccccgtag
     7381 cgcgagcctt attgcgttct acaggtccat tggcggtgac gtcagccaat atctccggtc
     7441 aacccagtcc atcgactgcc caggatgttt ttgagcagtt gggtgggcga atcgaattga
     7501 tcattgatgg cggtactact cccggaggcg ttccctccac tttggttgac tgcaccggaa
     7561 gtgaaatcag ggttctgcgt gagggaccca tttcattgga gcaaattctc tcagcgattt
     7621 ctcagtgaat aaccgcccaa actctcaccc tgcttttaat tctagctcag actagattta
     7681 gttccatgca tataatgaaa agtgcattct cctaaacaaa ccgccgatgg ctcgggcaag
     7741 ccaaaggcgg tttgatcttt taatgcctag aagcgtttag gataacggcc atgttggctg
     7801 aacatcaata tccagctcag agaaatggcc tgaaataaaa cgataatgcc ctgccgctgc
     7861 gatcatagcg gcgttatccg tacacaacga aaacgggggt atgtgaactt tgaattccga
     7921 ttgagcctga aaggcctcac gaagcggtcg atttgcagaa acgccgcctg caaccaaaat
     7981 ttcccttgcc ccaagatcac gagcggcttt gatcgttttg ttgaatagga tatctacaac
     8041 cgtggcctgg aatgaggcgg caaggtcttc cacaggcaat gcgttgccat tcttctttag
     8101 gttctgaact tcatacaaca cggccgtttt tagtccgctg aacgaaaagt catacgggtt
     8161 atcgagccgg gcacgcggga acttgaagcg agtcgggtcg cccgtttcag aagccttctg
     8221 aatggatggt ccaccgggaa agggcaggcc cagcagccgc gccaccttgt cgaaggcctc
     8281 accagccgca tcgtccagcg tggagccgag gcgctgatac gtcagatgat cggtcatcaa
     8341 attcagctct gtgtgaccac cagagacaag caatgccatc agcgggaatt taggttcatc
     8401 cggcacattt tcgctggcct tataaatcca ggaagaataa atatgccctt cgaggtgatt
     8461 gaccccaata agaggcaatc cggtccccag tgcgagtcct tttgccatgt tcatgccaac
     8521 caccagcgag cctgccaagc ctggtccacg cgtggcagcc agggcatcga tatctttcaa
     8581 tgtaagattg gattgggcca gtgtttgctc tacaaccggg ataatactaa gcacatgctg
     8641 gcgcgaggct acttcgggaa agacaccgcc ataacgcgcg tgtatgtcca tttgagaggc
     8701 cactgtggat gcgagcaatg cacgtccatt ctcgatgacc gcgcaggcgg tttcgtcgca
     8761 ggatgtttcg atggcgagga tacgtgtcag tttttgatta gacataattg agtcatgtca
     8821 ctgcgaggag caatggttgc gacgaagcag tctcatcatt agtaggagat tgcttcgggc
     8881 aaaaaccatc gccctcgcaa cgacagtctt aacccttctt ccattttttc atcggtaata
     8941 caaaatcata tggatactca gccagtttct ccatggtgcc gtctgggcgg atccagtacg
     9001 agtcttcaat gcgaacgccc atcccttttt caggataata taagccgggt tcaacggtga
     9061 tcacaacgcc tggtttgagc agattattgt cagaagcgct tgggctgctc catggacgtt
     9121 cgtgaatatt gagacccaca ccatgaccca gggaatgaac ataaccatct gtcacgaccc
     9181 cgtccatgtt gagtggagtc ttatggccgt ttttcccgaa ttcatcacaa accatcctgt
     9241 tatattcttt gaagcttaca ttcaaatcaa tattttcaat taccttgtcg tacacatctt
     9301 tcacttcatc atacaactgc tgggcttcag gcgtggcata tcccaaactc cacgttcgcg
     9361 tgaagtcgta ataataccct ccacctgctt ctgcagggta gatgtcaaaa acgatcgtct
     9421 gtcccagctt catcaactca gatgcggtgc ccgctgaatg cggaaccccg gcatcacgcc
     9481 ccgtggcgaa aataaaaccg gacggcaatt cagcaccctg ctctgaaacc cataagcgga
     9541 ttttggagtg cacctctccc acagtgagcg gggttccatc ttccttcaac aatacttcgt
     9601 catcacgaac atcgcaacct gtaagatatt cccgaacttt gtccaccacg agagtggttg
     9661 ccttacccat actgcgaata cgggcggcct ccgattcgtc cttggcttcc attgcataca
     9721 taaaaaggct atcgccctga tcttcgccga tgagttcaat atccggtagc tcatccttga
     9781 ttcgattttc aatatgcaaa accatgctga catttgtctc accatagacg ccaacacgac
     9841 cagaaactag atcatgttcc ccaaagatct ctttcggtcg ctgtgagagt tcagtcatgg
     9901 cagcttgtcg cgtcggcacc acctttagac cacttctttc cgcttcttcg cgttccatgg
     9961 cattgacata taaaactgca ggttgcccta tctttttgat caggactgct ttgcttacat
    10021 gaccgccacc cgtcaaataa tacatcggcg ggttatgttc tgcatttccc aatatcatca
    10081 aggcatccag atttcttaat ttcataagtt cgtcaagatc tgatttcata cgactcctga
    10141 ttcaatattc agtttcttaa attcattcgc aacttcatcg ttttcaatcg ttacgtgcag
    10201 ggtttgctta ttctttgata acttcgcagt gaatcccatg atctgctcta cattggcgaa
    10261 taccataaat gccgtgagag ggcgtttcca aagcaactga ccataagcgg gtgcaaacat
    10321 cagcttcaac aggagttcga cgattgttcc accaagtatc ccggcgatta aaccgacgaa
    10381 cgcaccgagc aaaagtggca gactctctgg ctgtgacgtt ggaccgtcag gcgtgatcaa
    10441 ccagacgggg ataaatgcag ccacgcacac cagcaatccg gaaataaaaa atggaagcca
    10501 ggtgatgtta gcaatcttat cttcctttgc cgcgcagtcc acgcacaatg gcacaggtag
    10561 tgcgagttca accatctgtc cgcgtttttg ttcgcctgtg gtcaatttca gagtccagat
    10621 gcgcacactt ggttttccac aatttacaca acgctcaggg aatttcagca cgggtggctt
    10681 ctttttatcc ttgataggaa catcaagact aaccacgcag tgccactttc aaggcgttca
    10741 acaacgcttc atcctgcaac acggttcgcg ggtcgaggag caccgcttga ttctcaaccc
    10801 gagcaatgac cggtggatta ccttcacgaa gcttcttcag gaatttatcc gggcccttca
    10861 cagtcaatga caacacatac gtgggcaggc tttcctcagg aaggcttccg ccaccgaccg
    10921 ttgattcgga tttgatcact tcgccctgac ccaattccgc ttgccacgcc tccgcccgga
    10981 ccctgacctt ctcaggtgtc aacgatatca ttcgccagat cgggattttc ctctctgcct
    11041 catccttgag atagtgaaat aaagttgcgg tgatgccggc caaacaagtt ttatctgcac
    11101 ggacagccct cgccaaggga tgctttttga tcttcgcgat caaatctgcc ttgccaacga
    11161 taattccagc ctgaggtcca ccgagtaact tatcacctga aaaacaaacc agatctaccc
    11221 cagcagccag cgactcttga acagtcggtt cgtgcgcgag tccgtaggtt gatgtatcca
    11281 ggagtgcccc tgagccaagg tcatccagaa gggggatatt tgctttatgt gcaacactaa
    11341 ccatatcttt gaggtcaggt tcttcggtaa acccgacaat cttaaaattg gaacgatgcg
    11401 cgcgcattac aagcgcagca gggtcttcga gggcttcctc ataatcagat aacctgacct
    11461 tattggtcgt gcctacttca accagcttcg ctcccgagcc cttcatcaca tctggaacgc
    11521 gaaagccgcc gccgatctcg accagttgcg tgcgtgaaat gatcacccgt ttcttgtttg
    11581 ccagtgccga taacacaagc aaaactgcgg aagcattgtt gtttaccacc agagccgctt
    11641 ctgcacctgt gagtttcttc aaaacagatt ctgcgtgaac cagccgcgaa ccgcgctgac
    11701 cttttgtaag gtcatattca agattggaat aattccgcga tgcttcctcc atggcacgga
    11761 tgctttcttc gctgaggggt gcacgtccaa gattcgtgtg gagaatgacc ccggtggcgt
    11821 tgatgatcgg ttgaagcgtg gcgctcgtcc atgtagagag gtgagcctcg gcccgggcca
    11881 ggatgacgtc gttggatggc aatacagctt cagcgtcctg tttgaattgc gagcgggttt
    11941 cgtccagggt gatgcggatt gaatccaacg ttaagggtcg gccatacgtc tcaatcaaat
    12001 gcccggcact ttgaaggaga acatccacgg atggaagatc acgaagattg atcatcggag
    12061 gtaatgggag gtttccagtg agctttttca cgcaggcgta tgaagtattc aatggcaatc
    12121 aagccacctg ccagcccaag aatgacatag actgtgacga gccgcccgag ttgaagccag
    12181 gccagggtcg cgccgtaaat gccaaaccac atcgcctggc ggatgatgac gttcgcttcg
    12241 gctggtgggc cacctggaaa gcgtcgatgg aaaaaataga cgatcggcaa catgctaccg
    12301 gtgatcgccg caattagcaa aatgaagaaa ccccatcgat accagacgaa cgggaaagcc
    12361 aggttgacga tggtccacag accaccccat ccaattacga acagtaacaa agatgcaaga
    12421 gcaaaggact taaaattatg gggtgattcc acgcgataga ttataccttt tggacacttt
    12481 gttcgcgctg agcggagatc tgagtgcgac ttgcgaagat cgtaggcgaa gcgcggttag
    12541 ttaatctagt aatcaataca ggcagggaca acgtatggag gtgaaaatac acagccgaac
    12601 cgaaatccgt tttcaaaacg gataataata aatggttcga gttcaccata attcacaacc
    12661 gggcgctgta cgggcaaggc atcagggtct gagccaatgg tgtacgtgta tggaccatcc
    12721 gcatttgcga cgaagccttc caccgattgg agtggccggg gcaattcatc acgggaggag
    12781 gcctgcattc cttcctgcaa caaatcctcc atgcgagcca gagattgacg gacctcttgt
    12841 gggtaacggc tgaacgttcc ggcgagcaca gccagagcaa aggctatgac caccgtcaat
    12901 aaccttctag cgggagcaac ttgtctgtag gcggagacct ccagacgaga aattaaccat
    12961 cgaattagcc aaccgaaagg cagatagaaa aatacgcgcg gcagaaagac gaagaacaag
    13021 gcaattaatg caccggtccg gttaccggtc tcgttgatga tgatccataa ggcggagaca
    13081 aacgacccca ccagcgccga agcgatgatg ccaggcaacg cttcctctgg ccaggctgcg
    13141 atgagtccta acgcgccaaa catcaaggtt gtgatgagga tgacaccgac cgtgccgggc
    13201 catggcacaa aaagtgggag atttggaaga aaaaactggt tgatgagatt tgaggtaaga
    13261 gaaaatccca acccgagaac aagcccgaga accagtccca ggcggcgctg tgaattaagt
    13321 tgcatgcttt actcctttga agcctgcgta ttatatcagc ccttattttc ataggtaaat
    13381 cggtataatg tgagaagata ttttttagtc tcagtagact ttcgattgaa agtggtggtt
    13441 tcggctgttt acgctcaacc accggagtta tgaggttata tgaatgtatc tgatgcgatt
    13501 cgattgaaac gcgcggtgag aaaattccag gagaagtctc tgcctgaaga tgtgattcgc
    13561 gccatcctca acgcggggcg acgctcacag tcatccaaga acgagcaggg atggcaattc
    13621 atcgccatac gcgataaagc catcttgaag gccctttccg aatgcggcac ctatgccggg
    13681 catctcgccg gcgcggcgtt aggagttgcg attctcaccc ctaaccctga agggaaattt
    13741 caggacttgt tcgatgccgg tcaaaccgcc gcctatatgc aattaatggc gctggaattg
    13801 ggagtcgggt cgtgtcttgc atcgatttat gaaccggcaa aggcgcgcga gattttagga
    13861 tttccactcg attggcattt gcgcatcgcg atctcgttcg gctatcccgc cgacgcagag
    13921 aagctcactg cctcaccgaa gaaaggcgga cgcgcacccc tggaggacct tgtccattgg
    13981 gatcagtggc aagacgaaat tcgtgacaac cagaggtcag atggatgaga tgaagttcac
    14041 acagcttgta gaagtgatgg gacgctggaa ggctgagatc cttgaggggc ttttgaagaa
    14101 cgagggaatc gacgtcgtgc taatccagga ttcgatttca agctctacct acgccaaccc
    14161 gtttgcgcct gtacaggttt ttgtccctaa cgaagccctt tcacaggcgc aggagttact
    14221 ggaagatttt gaaacgacag aagaaacaat ggaagatgag gatgagagcg gagatgagga
    14281 ataaacacct caatgctttg caaagaattg cgttgatttc tgaaatactg tatagttaag
    14341 gcgaaatccc catctttaag tacagaggag atagatcatg ccagatagtg tttataaggt
    14401 cattgaatta gttggcacca gcacagagtc atgggaacga gcggcaacgg tcgctgtaga
    14461 gcgtgcttcg caatccctac gtgacttgcg cattgcagag gtcgttcaat tggatatggt
    14521 tatcgatgaa ggcaaggtca cagcgtatcg tgcgaaagta aaagtctcat tcaagtacga
    14581 gagcggcgga tagtatccgt ttgaaaaggg ggcagatgat ttttttaatt ccattcctat
    14641 ctacaaaact ccttgaagag gtgtcaaatc caggagaatg gttttatttt gtttgtcttt
    14701 gaaccgctgt cctcggcggc gccgagagca cattgcaata cgaaaaggag aagtttatga
    14761 ctgcttatgt cattgtggac atccaggtca ccgatccagt tggttacgag gaatataaaa
    14821 agcttgcaac ctcgaccgtt gaacaatatg gaggaaaata cattgcccgc ggcggcaaga
    14881 ccgaaatcct tgaaggcgag tggtcaccgg gcaggctggt gattctggag tttgaaaatt
    14941 cagaacaggc gaagaaatgg cttaattctc cggaatacaa ccacccaagg caattgagac
    15001 acaagaccgc aaccagcaat atggtcgtga tcgaaggggt ttgatagaat tacatccatg
    15061 tttgagcttg agagctacat aaagcaattt gcagataaga caattgccat cgcggcgtac
    15121 gatttggaag ccggaacaga aatcctgatc aacgcagatg agtcctttca ccctgccagc
    15181 acgatcaaag ttcacgtgat gatggaggtc tttcgtcagg cggaggcagg attgctgtca
    15241 ctagatgatt gcatacctgt aatcaattca tttacaagca tcgcggatgg aagcaaattc
    15301 gggctggatg taaaagacga ctcagagacc acactgtatc aacgccttgg tgagagcgaa
    15361 agcattcttg agttgacgct attgatgatc gtacgtagta gtaatcttgc caccaatatt
    15421 ctgctggaaa aagttggcgc gaaaagcgta caggattttg ttcatgaatt gggtattgat
    15481 ggcgtaatcg taaaacgcgg cgtagaggac aatgtcgctt tccgcctggg tatgaataac
    15541 agcgccaccg cgcgtggtct tacgcaaacg atgcaattac tcgcggaggg aaaagtcgta
    15601 tcagagaaag cctcagccca aatgatcgag atcatgctcc aacaggagtt caacgaaagc
    15661 attcctgcct tgcttcctgg ggcagctaag gtcgcgcaca agacgggctg gaccagtgag
    15721 ttttatcatg acacagggat tgtatttccc gaaaaccgca aaccttatgc aatttcactt
    15781 atgacacgtg ggtttgcaga agccaacgaa gcccaagcac acgattgcat ggcaaatatt
    15841 tcaaggataa tttacgaaaa acttcaatga ttacaatttc ttcactcact tactatccca
    15901 tcaaagcctg ccgcggattc gaaattgaat ctgcaaatat cgaacgaatg ggattacaaa
    15961 atgaccgccg tatgatggtc gtctcgcctg aaggcggaca cctcacacaa cgagagcatc
    16021 ccaggctggc gctggtgact ccaaagctgg agaatggcac ggtcacactc tctgcgccga
    16081 attatgattc ccttcaattt gcgacacaaa caaccggagc gacgtacccg gtcagtattt
    16141 ggaaaagtaa aggcgtgcaa gccattgacc agggtgatga cgctgcccag tggttttcag
    16201 attggctcgg gacccctgtc cgattggtgc atatcgctgg tggattgcaa cgtcgtgtcg
    16261 atggcgaata tgcggttaat gctgacgatc acaccgggtt tgcagatggc taccccatcc
    16321 ttgtcacatc tgaatcaggg ctacaggatc taaat
//
LOCUS       DASTJP010000281         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_544770, whole genome shotgun sequence.
ACCESSION   DASTJP010000281 DASTJP010000000
VERSION     DASTJP010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544770"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2081
                     /locus_tag="VFQ23_08490"
     CDS             <1..2081
                     /locus_tag="VFQ23_08490"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906666.1"
                     /translation="TGKTRLSLQVAQELVDHFPNGVYFVPLADDRDANQFISRVAQQL
                     EVREGGRPLLENVKDYLREKQILLVLDNFEQLVSAAPVIADLLTSAPQLKIITSSRIA
                     LKLQGEYEYPVPPLELPQAENAGSLETLLGNEAVALFIARARAVHPAFALTKDNASTI
                     AEICRRLDGLPLALELAAARVKLLQPQAILSRLEDSLKLLTGGARDLPSRQQTLRNTL
                     EWSYGLLHEEEKTVYARLGVFVGGFTLEAVEAVCNPDGKLDILEGLTSLVNNSLLRQE
                     ETKDGEPRFGMLEIIHAFALERLAESGEIEKLREQHAGYFGDVIANQIGFFELYASRA
                     LYWLNWLEQEHDNIRAALTWYLSTPQGVEFSATLVNILFWFWYRRGYSIEAGMWAERV
                     LTSPSMQAPSFSRAMTLFASASSSMWQGNQNTALVKVQESLDIMHQIEDKQWMPFILM
                     GNAVVLINMGRDSDAQPLMQQALPIFKENKITPFEVITLVHLGNAELGLGHLEQARAR
                     HEEALAIARTLNENWLMSFALNNLGEVARTQGQFAQARKYYEESEQLLSNTGDKGDMA
                     RFVHNLGYIAQHEEDFELAEKQFRKSLAMFRRLGNRRGIAECLAGLAGLRAQQGQTEW
                     GATLLSAAESLLTVTGSAWWPADRVEVERNREMLRSALGAEAFEKAWKKGNEMTLDQA
                     IHFATDEPKT"
     gene            2065..2895
                     /locus_tag="VFQ23_08495"
     CDS             2065..2895
                     /locus_tag="VFQ23_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906667.1"
                     /translation="MNQKHNFGVGSTKEFSLRVSIASLVRVIFQHPSDGEVMLALERK
                     ATLREADREHTVEVKSQPFGGAVRIQDLSLLHDLIGDFHFDSESSLLEQDFRIFIRPS
                     AWPMLRDFCLLQFNSDDETFLETNPERELAEEFADALQINLNPEQYDCAPVATLIENE
                     ATPTNNFYSKRSPTVRLYRIFEAVITDPLLIDRMLKNSEGISDGEMRELAWADFHNGG
                     HGWANAFLVLPLKNITDCYLDLSPKERNEPFFCGNNLLDDSVAAILESVPVPKYQRQA
                     "
     gene            2922..3671
                     /locus_tag="VFQ23_08500"
     CDS             2922..3671
                     /locus_tag="VFQ23_08500"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET9906668.1"
                     /translation="MKAKTPRFPKYHREVVQALTRIGNATFGKAVQQDRGSQLIHLGI
                     SVPELRKRVRQGFSFYELQEDEVLAVWDDLWQNSRYGDVLFAALEFYTPRAKKQVNPN
                     LWSVVRHWSKRVDNWCHSDGLSALYSRILESNPAEVYPQMLEWNQSKSEWLRRISLVS
                     LIHYSGKNAVFLPLKKVLPLITNCLEDERYYVQTAVGWVLREMGNVYEKEVLTYLETH
                     IKRIRSSAFSRAIERRNAKDRAQLRKLRSAA"
BASE COUNT          974 a          913 c         1004 g          885 t
ORIGIN      
        1 gcaccggcaa gacacgcctc agtctgcagg ttgcacagga actggtggat cacttcccga
       61 acggcgttta ttttgtccca ctggcggatg atagagatgc caatcagttc atctcacgcg
      121 tggctcagca gttggaagtt cgcgaaggtg ggcgaccttt gctggaaaat gtaaaagatt
      181 atctccgcga aaaacagatc ctgctggtac tggataattt tgagcagctt gtgtccgccg
      241 cacctgtcat tgctgacctg ctcacctcgg ctccacaatt aaagatcatc accagcagcc
      301 gcattgcgct caaattgcaa ggcgaatacg aatacccggt tccgccgctg gaattgcctc
      361 aggcagagaa cgcaggcagc ctggaaacct tacttggaaa cgaggcagtt gccctgttca
      421 ttgcacgtgc gcgggccgtt catccggctt ttgctctgac gaaagacaac gcttccacca
      481 ttgcggaaat ctgccgccgt ttggatgggc tgccacttgc actggagctt gcggcagcac
      541 gcgtcaaatt gctgcaaccg caggcgattc tctcacgcct ggaagacagt ttaaaactgc
      601 ttaccggtgg cgcgcgtgat cttccatctc gtcagcagac ccttcgcaat acgctggagt
      661 ggagttatgg cttattgcac gaggaggaaa agacggtata tgcccggctg ggtgtgttcg
      721 tgggtggctt cacactcgaa gcggttgagg cagtttgtaa cccggatggg aagctggata
      781 ttcttgaagg tcttacgtct ctggtaaata atagcctgtt acgtcaggaa gagacaaagg
      841 atggtgaacc acgctttggg atgctcgaga tcatccatgc cttcgcgctt gaacgcctgg
      901 cggagagtgg agaaatagaa aagctgcgcg aacagcatgc tggctatttt ggcgacgtga
      961 tcgccaatca gatcggattc tttgagcttt atgcatcgcg cgcgctgtac tggctgaact
     1021 ggctcgagca ggagcacgat aatattcggg ccgcgctaac gtggtatctc tccacaccgc
     1081 agggagttga gttttccgcc accctcgtca atatattgtt ttggttttgg taccgccgcg
     1141 gttactccat cgaagccggg atgtgggcag aacgcgtcct gacctcccca tccatgcagg
     1201 ccccttcgtt cagccgcgcc atgaccctct ttgccagcgc ctcatcgtcg atgtggcagg
     1261 gaaaccagaa taccgctctg gtaaaagtgc aggaaagcct ggacattatg caccagatag
     1321 aagacaagca atggatgcct ttcatcctca tgggaaacgc ggtcgttcta atcaatatgg
     1381 gcagggatag tgatgcacaa cccttgatgc aacaggctct gccgattttc aaagaaaaca
     1441 agattacgcc ctttgaggtt atcacgctgg tccatcttgg gaatgcagaa ctagggttag
     1501 gtcatcttga gcaggcgcgc gcccgccatg aagaagccct ggcaattgcc cgaacgctga
     1561 acgaaaactg gctgatgtct ttcgcgctca ataaccttgg cgaagtggcg cgtacacagg
     1621 gacaatttgc gcaggcacgg aaatattacg aggaaagcga acaactgtta agcaacacgg
     1681 gggacaaggg agatatggct cgttttgtac ataatttagg gtacattgca cagcacgagg
     1741 aggatttcga gctggctgaa aagcaattca gaaaaagcct ggcaatgttc cgacgtttgg
     1801 gcaatcggcg cggaattgcg gaatgcctgg ccgggctcgc tggcttaaga gcccaacaag
     1861 gacaaaccga atggggtgcc actctgctca gcgcggcgga gtcgctgttg acagtcaccg
     1921 gcagtgcctg gtggcctgcc gatcgcgtgg aagtggagcg caatcgcgaa atgcttcgat
     1981 ctgcgttagg cgcggaggcc tttgaaaagg cgtggaaaaa aggcaacgag atgacacttg
     2041 atcaagcgat ccacttcgcc acagatgaac caaaaacata atttcggtgt tggctcaaca
     2101 aaagaattca gtctacgcgt aagtattgcc tcgttggtaa gggttatctt tcaacacccg
     2161 agcgatggtg aagtgatgct ggctttggaa cgcaaagcga ctctgcgcga agcagatcgc
     2221 gagcacaccg tcgaagtaaa atctcagcct tttggtggag ctgtccgcat tcaggatttg
     2281 agcctgctac acgacctgat cggcgacttt cactttgaca gcgaaagctc actccttgag
     2341 caggattttc gcatcttcat tcgaccttca gcttggccaa tgcttcgaga cttttgcctc
     2401 ctacaattca acagtgatga tgagactttt ctcgaaacga accccgagcg tgaattggcg
     2461 gaagagtttg cagatgcact gcagatcaat ttgaaccctg agcagtatga ctgtgcgccc
     2521 gtagcaactc ttattgaaaa tgaagcgacg cccaccaata atttctactc gaagcgttca
     2581 cctacggtaa gactgtatcg tattttcgaa gcagtgatta cagaccctct cttaatagat
     2641 cgtatgctaa aaaacagcga aggtatctcc gatggggaga tgcgtgaact tgcatgggca
     2701 gattttcaca atggcggaca tggatgggca aacgcgtttt tagttctgcc attgaagaac
     2761 attacagatt gttatctgga tttatcgcct aaagaacgca atgaaccttt cttctgtgga
     2821 aataacttgc tggatgattc tgtggcagca attcttgaga gcgttcctgt accaaaatat
     2881 caaagacagg cgtgaaataa ctccaaccca tggaaccgca aatgaaagca aaaacgcctc
     2941 gattcccaaa atatcaccgt gaagtggtac aggcactcac ccgcattggc aacgcgacct
     3001 ttggcaaagc cgttcaacaa gatagaggct ctcaactgat acaccttggc atttctgtcc
     3061 ccgaattaag aaaacgagtc aggcagggtt tttcttttta tgagctacag gaagatgagg
     3121 tgttagccgt atgggacgat ttatggcaaa actctcgcta tggagatgtg ctctttgccg
     3181 cgttggaatt ttatacccct cgcgcaaaga aacaagtgaa tccaaatctt tggtcggtag
     3241 ttcgtcactg gagcaagcgt gttgacaatt ggtgtcacag cgacgggttg agcgctctct
     3301 actctcgcat cctggagagc aatcctgctg aagtgtatcc gcaaatgctc gaatggaatc
     3361 aatcaaaaag tgaatggttg aggcgtatat cgctggtcag tttgatccat tacagcggca
     3421 agaacgcagt gtttctgccg cttaaaaagg ttttgccact catcacaaac tgccttgagg
     3481 acgagagata ttatgtccaa acagcggtag gttgggtatt gcgagagatg ggcaacgtat
     3541 acgaaaaaga ggtgctgacc tacttggaaa ctcacatcaa gagaatccgc tcatcagcct
     3601 tttcacgagc gattgaaaga cggaatgcca aagaccgtgc gcagttgagg aagttgcgtt
     3661 ccgcagcata aaacaaaacg gggctgtgga tccacagtcc cgttttgttt cgtcagatgg
     3721 aggacaggtc agaactactt gattaccgcg ctgcttgcca gagcaaacag gaattg
//
LOCUS       DASTJP010000282         6703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1166706, whole genome shotgun sequence.
ACCESSION   DASTJP010000282 DASTJP010000000
VERSION     DASTJP010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166706"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1684)
                     /locus_tag="VFQ23_08505"
     CDS             complement(35..1684)
                     /locus_tag="VFQ23_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906669.1"
                     /translation="MRNSNGRRAITILIAFLVSLALPTVAFAHPLGNFTINHYAGLNV
                     SRELVEIDYVLDMAEIPAFQEIASFDANGNGQPDATETAAYHSARCESLRSDLNLRVN
                     NQATPLALASSKIEFPAGAGGLLTLRLTCAFRAALTEETTSISFTDNSYTERLGWREI
                     VVTSDEVGLQGDFASSSLSQRLTIYPEDMLSSPLDQREISFAVDLSSAGMSSPATEQQ
                     ASSLSTGREDAFTRLITLEQLTLPAVLLALGISFVWGAAHAMTPGHGKTIVGAYLVGS
                     RGTVKHALYLGLTTTITHTLGVFALGLVTLFAAQYIVPEKLYPWMSLLSGLFVVGIGL
                     NLFVQRFRSSKLGGWLNGHKPSLSSLQPIYSTATQSTKLNTERVHAHRFVPTSTPGHH
                     HAHDGHSHDHPHPHGSDHIHTDDEHLHDHVHDNHGHHHDHGPGGHSHTPPEEITWRSL
                     LGLGISGGLIPCPSALVVMLGAIALNRIGFGLVLVFAFSLGLAGALTAIGMLFIYAGR
                     LFARFPSQAKVLQILPALSALFVAAIGVAISVRALAEIGLV"
     gene            complement(1684..3006)
                     /locus_tag="VFQ23_08510"
     CDS             complement(1684..3006)
                     /locus_tag="VFQ23_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906670.1"
                     /translation="MNRLIILFFLVLLAVAVSWGIASNQAASASFNGSGEYPLEDASR
                     VSVSVDEQIQSLQSLLRENPNDWQSSSQLGFAYLQKARETGDPAYFQKVEDALGNALE
                     YHSDDYATVSGMGTLALARHQFHEALEWGERARQLDPERAYAYGVIADAQIELGQYEE
                     AVETLQTMVDLRPDMNSYSRISYIRELHGDTEGALEMMQSAVDSGVPNAESTAWTRTQ
                     LGNLYFNTGDYDQAELEYVRTLNDRPGYVYALAGLGRVRAAQGKTEEAIEFLTEATNV
                     MPLPEFVITLGDLYEVNNQSELAKRQYDLVGAIEQLHKANGVDMDMEIALFNADQDKD
                     LDVNVQRARLAFANRPSIHAADVLAWALYKVGNFEEAKFYSEKSLQLETKDALKLFHA
                     GMIAYRLGEHERAQDYLEQALTINPHFSILYSAEAQKTLDILQSQGDQ"
     gene            complement(3100..4803)
                     /locus_tag="VFQ23_08515"
     CDS             complement(3100..4803)
                     /locus_tag="VFQ23_08515"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HET9906671.1"
                     /translation="MRKFSYTMFVGLMVMALVVGLAPSLSQAASHREAPLISMDPNAD
                     ITDFFMFRSYEEGHDDNIVLIMNVNPLEEPSGGPYYYNFDPTVLYTFNIDNNMDGKAD
                     DVRFEFEFKSEIRGVNRDLGLFLGYVAVPGPITALDGPGSEGLGLRQSYTVTMIKDGK
                     RNMLARGLFAVPSNVGPRTMPDYEALVQQGIYDLKDGARVFAGQRDDPFYIDVGAIFD
                     TLNLRDPGVDQLSGFNVHTIALEVPASWLTADEGGPADTESPVLGAYSSTYRRSTTVL
                     RENRDNKEDADTDRENARRGSGNWVQVQRLANPLVNETIIGTVDKDRWNSLDPSKEQR
                     FLDYYLKPRLALALQLVVGLDTGCTPFGDANCQPNPPAAADTTLAAFNRTDLVNILLK
                     YSPDDKKLSELLRLDISTSPVPLVSQDRLSVLAGDNAGWPNGRRPIDDVTDIAVQVVG
                     GPNYIAAGAGDNVNENDMPLPDMFPFLSTPWDGRDHVHENPDGALTPTPVGTETGTAT
                     GTVTPTLDGSVTPTGTTVTPFTATPSATNTPTATLSTTPTGSATFTPTSTATNTPSPT
                     P"
     gene            complement(5220..6578)
                     /locus_tag="VFQ23_08520"
     CDS             complement(5220..6578)
                     /locus_tag="VFQ23_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HET9906672.1"
                     /translation="MRKFSYTTLVGLLVLAFVVGLAPAATQAASHREAPLISLDPTAD
                     ISDFFMFRSYEAGQDDKIVFIMDVIPGEEPSSGPNYFNFDPNVLYAFNVDNNTDGKAD
                     DVRFEFEFKTELRGVNRDLGLFLSYVALPPITALDGPGSEGLGLRQSYTVTMVKGNNR
                     TVLGKGLFAVPSNVGPRTMPDYDALAAQGIYNIKNGARVFAGQRDDPFYIDLGAVFDT
                     LNLRDPGTDMLSGFNVHTIALEVPASLITKDGKNPTNTAFPTLGAYANTYRRSVKVIN
                     EDGESTKDRWVQVQRLANPLVNEAIIGTVDKDRWNKRDPRNESAFVEYYTNPRLATAL
                     EVVFGADAQPLLDLRDVLLTYTPGNYDRLSELLRLNISVAPVALADQNPLTVLGGDNA
                     GWPNGRRPIDDVTDVAVRVIGGTNYLSASDAVQVNDMPLPESFPFLATPWDGRGRVHQ
                     NP"
BASE COUNT         1707 a         1661 c         1715 g         1620 t
ORIGIN      
        1 gggacaagca gctgcttgtc cctacaaatt gttattaaac caatccgatt tctgctaatg
       61 ctctgaccga aatcgcaaca ccaattgcag caacaaataa ggcgctcagc gctggtagga
      121 tttgaaggac ttttgcctgc gaggggaatc tcgcaaacag ccgtccggcg tagataaaca
      181 acataccgat ggcggtcaac gcacccgcaa gccccaggct aaatgcgaac actaaaacca
      241 gaccgaaacc aatccgattt agagcgatcg cacccaacat cacaaccagg gcagatggac
      301 atgggatcaa ccctccggaa atgccaagcc ccagcaaact gcgccatgtg atttcttctg
      361 gcggtgtatg cgagtgacca cccggcccat gatcgtggtg atgtccatga ttatcgtgta
      421 cgtgatcatg aagatgctca tcatctgtat ggatgtgatc actaccatga ggatgtggat
      481 ggtcgtgcga atgtccgtca tgagcgtgat ggtgaccagg tgtagaagtt ggtacgaagc
      541 gatgggcatg aactcgttct gtatttaact tcgtgctttg cgtggcggtt gaataaatgg
      601 gctgcaaact cgaaagactg ggcttgtgtc cattgagcca tccgcctaat ttggaagatc
      661 gaaatcgttg gacgaaaagg ttcaaaccaa ttcctacaac gaacaacccg gagagcaggc
      721 tcatccacgg atataacttt tctggcacga tatactgggc cgcaaatagg gtgaccaaac
      781 ccaaagcaaa cacgccaagg gtatgtgtga tggttgttgt caaacctaaa tacagcgcat
      841 gcttgaccgt gccacgtgac ccaaccagat acgcccccac aatggtctta ccatgcccgg
      901 gagtcatggc gtgtgcggct ccccagacga atgaaatacc aagggctaat aagactgccg
      961 gtagcgtgag ttgttccagg gtgataagtc ttgtgaatgc atcttctcgg cccgtgctga
     1021 gagacgaagc ttgttgttcc gtggctggtg aagacatacc tgcggatgac agatcaaccg
     1081 cgaaggaaat ctctctctga tcgagtggac tactcagcat atcttctgga taaatagtaa
     1141 gcctctgact cagactgctc gaggcaaaat caccttgcaa accgacctca tcactggtaa
     1201 cgacgatttc tcgccagcca agtctctcgg tataagaatt atcggtgaat gaaatgcttg
     1261 tggtctcctc ggtaagagca gcgcgaaagg cacaggtaag gcggagagtc aacaatccgc
     1321 ctgccccggc tgggaattca atcttcgatg aagctaatgc cagtggcgtg gcttggttgt
     1381 tcacgcgtag gttcagatca gaccgaagag actcacacct ggcgctgtga tatgccgccg
     1441 tctcggtagc atccggctga ccgttgccat tcgcatcaaa ggaggcaatc tcctggaagg
     1501 cagggatctc ggccatatca agaacgtagt caatctcaac cagctcacgc gacacattca
     1561 aacccgcgta atgattgatc gtaaaatttc caagtggatg cgcgaacgcc acggtaggca
     1621 atgcaagaga taccaagaac gctatgagga tagtaatggc tcttcgcccg tttgaattgc
     1681 gcattattga tctccttgtg actgcaaaat atccagtgtc ttttgggctt cagcagaata
     1741 aagaatggaa aaatgcgggt tgattgtcaa tgcttgttcc aaataatcct gtgcccgctc
     1801 atgctcgccg aggcggtatg cgatcatgcc tgcatgaaat aatttaagcg catctttggt
     1861 ttctaattgc agtgattttt ccgaataaaa ttttgcttct tcaaagttgc ctactttgta
     1921 taatgcccag gccaatacgt cggcggcatg gatgctgggt cgattggcaa acgccaggcg
     1981 cgcacgctga acatttacgt ccaggtcttt atcctgatct gcattaaaaa gagcgatttc
     2041 catatccatg tccacaccgt ttgctttgtg aagttgttca atcgctccga ccaaatcgta
     2101 ttgtcgcttt gccagttcag actgattgtt gacctcatac aagtcaccga gggtgatgac
     2161 aaattcaggc aacggcatga cgttggttgc ttccgttaaa aactcaatgg cttcttctgt
     2221 tttcccctgc gccgcccgca ctcgccccaa acctgccaga gcatacacgt agccaggtcg
     2281 atcgttaagc gtgcggacgt attcgagttc agcctgatcg taatcaccag tattgaagta
     2341 aagatttcct aattgcgtac gagtccaggc cgtgctttca gcgttgggca cgccactatc
     2401 cacagcggac tgcatcatct ccagcgcccc ttctgtgtca ccgtgcaatt cacggatgta
     2461 ggaaatacga gagtaggaat tcatatccgg acgcagatct accatggttt gcaacgtttc
     2521 gacggcttcc tcatactgac ccaattcgat ttgtgcgtca gcgatgacgc cataggcata
     2581 ggcgcgctcc gggtctaatt gcctggcacg ttcgccccat tcaagcgcct catgaaactg
     2641 atgacgggcc aatgcaagcg tccccatccc actgactgta gcgtaatcat cagaatgata
     2701 ttccagtgca ttgcccagag cgtcttccac tttttgaaaa taagccgggt cacctgtctc
     2761 gcgggccttt tgcagatacg caaatcccaa ctggctggag gattgccagt cgttcggatt
     2821 ctcacgcagc aaactttgta acgattggat ttgttcatcc acagagacac tcacgcgcga
     2881 cgcatcctcg agaggatatt ctccacttcc attaaagctc gccgatgcgg cctgattgga
     2941 agctatgccc catgaaactg caaccgctaa gagaacgaga aaaaataaaa taataaggcg
     3001 attcatagtt cgattttgaa caattgtttg cataataatc tctgaagttt tggctaggtc
     3061 gggaaaattc ccgacctagc cattatgaac ttgaaattcc tatggtgtcg gtgagggtgt
     3121 attggtagcg gttgaggtag gagtaaaggt tgcactacca gtcggggttg ttgaaagtgt
     3181 tgcagttgga gtgttggtag cagacggcgt tgcagtgaaa ggtgtcactg tggtaccggt
     3241 gggtgtgaca ctaccgtcaa gggtaggagt tacggtaccg gtagcagtcc cagtctcagt
     3301 tccgaccgga gtcggtgtta gtgcaccatc tggattctca tgcacgtggt cacggccatc
     3361 ccagggtgta gaaaggaatg ggaacatgtc aggcagaggc atatcattct cattcacgtt
     3421 atcacctgcg ccagcagcaa tgtagttggg tccaccgact acctgaactg caatgtcggt
     3481 cacatcgtcg attggacgac gtccattggg ccagccagca ttgtcaccag ccaaaacgct
     3541 caaacggtct tgagagacaa gggggacagg agacgtactg atatctaaac gcaacaactc
     3601 ggagagtttt ttatcgtctg gcgaatactt tagaaggata tttaccagat ctgtgcgatt
     3661 gaaggccgcc agagttgtat ccgccgcagc cggtgggttt ggctggcaat tagcatcacc
     3721 gaacggtgta cagccagtat ccaagcctac gacgagctgc agggccaggg ccaaacgtgg
     3781 ttttagatag taatcaagga accgctgttc tttagaagga tcaagtgaat tccagcgatc
     3841 cttgtcaact gtgccgatga tcgtctcgtt cacgagtggg tttgccaggc gttgaacctg
     3901 cacccagttg ccacttccac gtcgtgcatt ttcacgatcg gtatcagcat cttctttatt
     3961 atcgcgattt tcacgcagta cggttgtact gcggcggtag gtactactat aggcacctaa
     4021 cacaggcgat tccgtatccg ctggaccccc ttcatccgca gtcagccagc tcgctggaac
     4081 ctcaagagca atcgtgtgaa cattgaagcc agacaactgg tctacacctg ggtctcgcag
     4141 attaagcgtg tcgaaaatcg cgccgacatc aatgtagaac ggatcgtcgc gctgacccgc
     4201 aaaaacacgc gcgccatcct tcagatcgta aatcccttgc tggacgaggg cttcataatc
     4261 aggcatcgtg cgcggcccaa cattggacgg aacggcaaat aagccgcgcg ccagcatgtt
     4321 gcgttttcca tccttgatca tggttacggt ataactctga cgcaaaccaa gtccttctga
     4381 acccggacca tcaagagctg tgatcggacc tggtacagca acataaccta agaataatcc
     4441 caggtctctg ttgaccccgc ggatttcgga cttgaattcg aactcgaagc gcacatcatc
     4501 agctttaccg tccatgttat tgtcaatgtt gaatgtatat agcactgtcg ggtcaaagtt
     4561 gtagtaatac ggccccccgc tgggctcctc aagagggttg acattcatga tgagtacgat
     4621 attgtcgtca tgaccttctt cgtaactgcg gaacataaag aaatctgtga tgtccgcatt
     4681 cgggtccatg gaaatgaggg gggcttcgcg gtgactggca gcttgtgaga gagagggcgc
     4741 cagaccaacg acgagggcca taaccatcag tcccacgaac attgtgtaag aaaacttacg
     4801 catatcagtt aatctccttg ctcggtatat gctattctcc tggttatgag acaactcctt
     4861 cgaactcatg ggcctatcat ctaattcttg gtgaattccg ggagtctgtg atctaattta
     4921 gtttcaaggt cttagagaat atcaggcatt aacacctcct attgctttat tgctggccgt
     4981 cgcgctcgaa atatggtcag caaaaagtga tgtaataccc gatcattatg gtggttggct
     5041 tccctagggt caatcaggtg ttaccctact tgaccttaac cttcggtgag agaatctttc
     5101 ttcacttacc aaaagctcat aacgaaacaa aaaagagtaa tgagaacggg ataaagactg
     5161 caggaaaaga catgtgagag tgagccgagg tctgcccggc tcactccctt cacttagtgt
     5221 tacggattct gatgaacgcg accgcgtccg tcccaaggtg tagccaggaa tgggaaggat
     5281 tccggcagag gcatatcgtt cacctgaacc gcgtcactgg cactcaaata atttgttccg
     5341 ccaatgacac gaactgccac atcggttaca tcatcaatcg ggcggcggcc atttggccag
     5401 ccagcattat caccacccag cactgtcagc gggttttgat cggctaatgc tacaggtgcc
     5461 acgctgatgt tcaaacgcag cagttctgaa agacgatcat agtttccggg ggtgtaggtc
     5521 agaaggacat cgcgcagatc caacaatggt tgagcatcgg caccgaagac cacttcgagg
     5581 gctgtggcca aacgtgggtt ggtgtaatat tcaacgaatg cactttcatt tcgcggatcg
     5641 cgcttgttcc agcggtcttt gtcgaccgta ccaatgatgg cttcattgac cagcgggttg
     5701 gcaaggcgct gaacctgcac ccagcgatct ttcgtgcttt cgccgtcttc attgataacc
     5761 ttaacggaac ggcgatatgt gttggcatag gccccaaggg tggggaatgc ggtattcgtt
     5821 ggattctttc catccttggt gatcagcgac gcgggtactt ctaaagcgat cgtgtggacg
     5881 ttgaagccgg aaagcatatc ggtgccagga tcgcgcaggt tgagggtgtc gaacacagcg
     5941 ccgagatcga tatagaacgg atcatcgcgt tgaccggcaa agactcgggc cccattcttg
     6001 atgttgtaaa tgccttgtgc ggcgagggca tcgtaatcag gcatagtgcg agggcccacg
     6061 ttcgagggca ctgcaaacaa acctttgcct agtacggtgc gattgttacc cttgaccatg
     6121 gtcacagtgt aagactggcg taaacccagg ccttcagaac caggaccatc gagggcagta
     6181 atgggcggca aagcgacata cgataggaac aatcccaggt ctctgttgac gccgcgaagt
     6241 tcagtcttga attcgaactc gaagcgaacg tcatcggctt tgccatccgt gttgttgtca
     6301 acattgaagg catacaagac attcggatcg aaattgaaat aattgggtcc ggagctgggc
     6361 tcttctccgg ggatgacatc cataatgaaa acgattttgt catcctgccc ggcttcgtaa
     6421 ctgcggaaca tgaagaaatc actgatatcc gcagtggggt cgagagagat caggggtgct
     6481 tcgcgatggc tggcggcttg tgttgcagca ggtgccaggc caacgacgaa ggcaagaacc
     6541 agcaaaccca cgagcgttgt ataagaaaac ttacgcatta ttaactcctc tttctaactg
     6601 gaaggaaaat ccttctgaaa tgttttgatt tacaaataag cttcagtgaa atactttaga
     6661 aagtatggga cattggcacc tccaggtgtg gaatcgccga ccg
//
LOCUS       DASTJP010000283         7575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_786466, whole genome shotgun sequence.
ACCESSION   DASTJP010000283 DASTJP010000000
VERSION     DASTJP010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786466"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..444)
                     /locus_tag="VFQ23_08525"
     CDS             complement(34..444)
                     /locus_tag="VFQ23_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906673.1"
                     /translation="MIKQPARLYTAASGIFLLLQGISTLAFRLYPPLDQAFPALLATT
                     HMIPIHSTLHIISGILAVVTLYRGGESGAFWFALGFGLFYLGLGVVGVISGQPLGLGL
                     QPFDHPIHLVLGVVGLIAAWLSFHRTTSMRKVAP"
     gene            complement(446..1234)
                     /locus_tag="VFQ23_08530"
     CDS             complement(446..1234)
                     /locus_tag="VFQ23_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906674.1"
                     /translation="MTVTRNQKIYFAAVGLLALWVGIWGYFVPERVDKAIPWLVPPLH
                     ARFIGAIYFSAVVLMGMSMLAKHPAEVRVPTFIVALWTGMLFIISLFYLSEFDFSRGP
                     VWFWFGAYILYPIIGFWLTWQYRNTESDPTALRLPKWITNYFLTQGILITLLALALLL
                     APDAMLAVWPWKITRMLAQIYSGPFLAYGLGSLMLSRQQTWREVRIVTMAFVLLSIGV
                     IIASVLHWALFSASNPSTWIWFAGLSLATLFSGIIIFRNSSAGD"
     gene            complement(1335..1706)
                     /locus_tag="VFQ23_08535"
     CDS             complement(1335..1706)
                     /locus_tag="VFQ23_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9906675.1"
                     /translation="MDIEEFIHAYEQALATQDWVNLDPLVHQDACVTFSNGTVHQGKP
                     AVRKAFERNFSSIKDETYSISNVHWVNKGSEMAVYLFEFNWSGLINDKPASGSGRGTS
                     VLIKDNDKWLLLVEHLGPKAS"
     gene            complement(1796..2182)
                     /locus_tag="VFQ23_08540"
     CDS             complement(1796..2182)
                     /locus_tag="VFQ23_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906676.1"
                     /translation="MTYLTVKPVSTNNTCRDEFINACRAMIRNGTPKKYMGRIANPES
                     VNADYADFWLAFDTKGEEMARQIIRNSGRSVEKGKYDFPGVVHLFEHTVPDPPEAGDI
                     KPWVSLFDSQLEPWKEKIKKMHLEHS"
     gene            2516..4051
                     /gene="ftcD"
                     /locus_tag="VFQ23_08545"
     CDS             2516..4051
                     /gene="ftcD"
                     /locus_tag="VFQ23_08545"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET9906677.1"
                     /translation="MTTQLIECIPNFSEARRPEVIDQIAAAITSVEGARLLDRSSDLD
                     HNRTVLTFAGPPEAVEEAAFRAIQTACELIDLNNHTGEHPRMGATDVVPFVPLSGTTM
                     EECVAIARRLGQRVGEELGIPVYLYEAAATRPERANLENIRKGQYEGLRSEIESNPDR
                     APDFGPSKIGTAGATVIGARNPLIAFNVYLTTSDVDIAKKIAKAVRHSSGGLRNVKGL
                     GLLVEGRAQVSMNLTDFRETPIARVVETIRREATRYGVGIHHSELVGLIPQDALVDAA
                     VWYTQLDGFDKEQILETRLHDAPASRFAQTDDSPSFIEELAAPTPTPGGGSAAAYAAA
                     MGAALVAMVAGVTIGKKKYAHVEASMQAVRVMAEKLRKELTLAVDDDASAFEALMATY
                     KLPKETEEQQKTRAAQIKSATLTAATVPLHVAEDAVKVMEIALKCAKDANVNAISDAM
                     SGFAMARAALTAAGYNVRINISTLEDKSVGDKMLNELAELEKKSEKVEKEIQAVMKER
                     GGI"
     gene            4119..5069
                     /locus_tag="VFQ23_08550"
     CDS             4119..5069
                     /locus_tag="VFQ23_08550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9906678.1"
                     /translation="MKLTALFFLIFFLTSCGTTTNVPDIATNTIVVPSPLPPTFTATP
                     TELLPTVTPQPTATQIPCDPSSADFCITDGHFIFQRPIKLLTNTSIDPTYPYGSTARG
                     TRDPHHGVEFQSPFGTPVYAAGDGVVLFAGADERAIYSPWPNFYGNLVVIEHENDLFT
                     LYAHLSRIDVQSGTVIQEGDKLGEVGQSGAATGSHLHFEVRRGVVEDYFSSVNPELWL
                     APTKNENGAIAISVVDQSAKFQIAELTISYYPDRSQPPVLSYYATTYARDLSTGEENA
                     AVSDVSPGNYRIAFTMNGRVYERWVEVKSGRLTQVVFVAN"
     gene            5158..5712
                     /gene="dcd"
                     /locus_tag="VFQ23_08555"
     CDS             5158..5712
                     /gene="dcd"
                     /locus_tag="VFQ23_08555"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET9906679.1"
                     /translation="MGLKPDHWIRKMAMEQRMIEPFVDKQVRQGVISYGVSSYGYDIR
                     VADEFMIFTNVHSAIVDPKNFDPKSMVEFKGDVCVIPPNSFALARTIEYFRIPRKVLT
                     VCLGKSTYARCGLIVNVTPFEPEWEGFVTLEISNTTPLPAKIYANEGLAQVLFFEADE
                     ECEVSYADKKGKYQKQESIVLPKL"
     gene            complement(5729..6613)
                     /gene="thrB"
                     /locus_tag="VFQ23_08560"
     CDS             complement(5729..6613)
                     /gene="thrB"
                     /locus_tag="VFQ23_08560"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET9906680.1"
                     /translation="MIKIRVPATSANLGPGFDCLGLALHIWNEVSFEESDSITYQVTG
                     EGADKLNHRPINLLTDSFKKVYEVCGKELHGIHIQAHNNILHSSGLGSSAAAIVAGIY
                     GANEILGAPLDIYALVKLATEIEGHPDNVAPALLGGLVVSVVTDREIITRRYEIPALA
                     IVIVKPDVQWQTRTARSVLPDTVSRADAIYNIGRTALVIDTLRNGDLDLLQKVMDDRI
                     HQPYRLKHIAGGLAAYEAARQLGAAALSGAGPSIICFVSREKAENAKEELIDVFMKQG
                     IQSQGLITKPSSSGAHKL"
     gene            6656..>7575
                     /gene="thrC"
                     /locus_tag="VFQ23_08565"
     CDS             6656..>7575
                     /gene="thrC"
                     /locus_tag="VFQ23_08565"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9906681.1"
                     /translation="MTYQGIIHRYRNLLDLPAHSQTVSLLEGDTPLIPMPRLSKQFGC
                     ELYVKFEGLNPTGSFKDRGMTAAISEAAGRGAQTVICASTGNTAASAAAYAARAGLRA
                     IVLIPDGKVAVGKLAGAVAYGAQVIQVQGSFDDALSLVVEITQKTPIALVNSINPFRI
                     EGQKTAAFEICDTLSRAPDWLCLPVGNAGNITSYWAGFKQYQKGLPQLLGVQAEGAAP
                     LVLGHPVDKPETIATAIRIGRPARGEYALEAAQESKGKLIAVSDAEILSAQKILAGEG
                     VWVEPASAAGLAGLIAETEKGSLDVKGKRVV"
BASE COUNT         1954 a         1868 c         1920 g         1833 t
ORIGIN      
        1 gcaggggcag ggcgatcggt gatccttgtg ggttcatgga gccaccttcc tcattgaagt
       61 tgtgcgatgg aagctcagcc aggcggcgat caacccgacc acgcctaaca ctaaatgaat
      121 cggatgatca aagggctgca atcccaaccc taatggttga ccgctgatta ctcctaccac
      181 tcccagaccg agatagaaga gaccaaagcc cagcgcaaac cagaatgcgc cgctttctcc
      241 gccgcggtac agggtaacaa cggccagaat gccagagatg atatgcaacg tcgagtgaat
      301 gggaatcatg tgggtagtgg ctaataaagc agggaaggcc tgatccagtg gtggataaag
      361 acggaaggct aatgtgctaa tgccctgcaa caacaagaaa ataccagaag ctgcggtgta
      421 aagacgagct ggttgtttga tcaagttaat ctcctgcaga cgagttgcga aagatgatga
      481 tcccagaaaa taatgttgcc agcgagaggc cggcaaacca gatccatgtg gatgggttgg
      541 aagcagaaaa gagtgcccaa tggagtaccg acgcgatgat gacgccgatc gataacaaca
      601 cgaatgccat tgtcacaatc cggacctcgc gccaggtctg ctgacgactg agcatcaggc
      661 tacccaaacc gtacgcgagg aacggtcccg agtaaatttg agccagcatg cgcgtgatct
      721 tccagggcca gacggctaac attgcatctg gtgcgagcaa taaagccagg gcaagcagag
      781 tgataagaat accttgcgtc agaaaataat tcgtaatcca tttcggcaaa cgcaaagcgg
      841 ttgggtcgct ttctgtattg cgatactgcc atgtcagcca aaagccaatg attggatata
      901 gaatgtatgc cccaaaccaa aaccagactg gaccgcggct gaaatcaaat tcgctgagat
      961 agaaaagtga gatgatgaac aacatgcctg tccagagagc aacaataaat gttgggacgc
     1021 gtacttcagc agggtgcttc gccagcatgg acatccccat gagcacgact gccgaaaaat
     1081 aaattgcccc aatgaaccgc gcatggagcg gaggtaccaa ccagggaatt gctttatcaa
     1141 cacgttccgg cacaaaatat ccccatattc ccacccataa cgccagcaga ccgacagcag
     1201 caaagtaaat tttttggttt cgagtcactg tcataggatc aggggcctct acgaaatcag
     1261 aatgagatga tggtgacggg cggcgtttcg cggtgctcgg atgattataa atatgttctt
     1321 atcgaatcac aatgttaaga ggcctttggt cccaaatgct cgactagcag tagccacttg
     1381 tcattgtctt tgatcaatac agatgtacct cgccctgaac cacttgccgg cttatcgtta
     1441 atcagtccac tccagttgaa ctcaaacaga tagacagcca tttcagagcc tttgttcacc
     1501 cagtgcacat tcgagattga atacgtttca tccttaatgc tcgaaaagtt cctctcaaat
     1561 gcctttctga ccgctggctt gccctgatgt accgttccgt ttgagaaagt cacacatgcg
     1621 tcctgatgaa cgagtggatc caagttcacc cagtcctggg ttgctaaagc ctgttcgtac
     1681 gcgtgaataa attcttcaat atccatggaa tttctcccgg gcggtcagta taagtcgaac
     1741 agagtataag atggaggtga tgaatcccac tcttgtggac aagtgttttc ccggactagg
     1801 aatgctccaa atgcattttt ttgatctttt ctttccaggg ctccaactga ctgtcgaaca
     1861 ggctaaccca tggctttata tcgcctgcct ctggaggatc aggaaccgta tgttcgaata
     1921 aatgaacaac tccgggaaaa tcatattttc ctttttcaac cgatcttcct gagttccgaa
     1981 taatttgcct tgccatttcc tcaccctttg tgtcaaatgc cagccagaaa tctgcataat
     2041 cggcatttac tgattccggg tttgcaattc ggcccatata ttttttagga gttccattgc
     2101 gaatcattgc ccggcaagcg tttatgaact cgtctcgaca ggtgttgttg gtcgacactg
     2161 gttttacagt aaggtaggtc atggttaaga ttgtggaagc cgcgacaacg atctctcccg
     2221 ctctgagacc gcgttcgctg ataattgcga tagtcattat tggcagcatg tttttaattc
     2281 tttcctgaaa tggatgaaac gcgctaagtt gtaaattttc gccccattct ctgtagatat
     2341 tgtatcacct gttgttgcaa ttgatgagac tttcccttca gtggattaat ctatcacgca
     2401 ggatcgttta gagatgtgtt tgtatggaag agcaataggc attcttcaaa atacaatttc
     2461 ctcccttata attaaccaga ccaatctcaa atctaaactg tacaacaaaa accatatgac
     2521 cactcaacta atcgaatgta tccccaactt ttccgaagcg cgacgaccgg aagtgatcga
     2581 ccagatcgcg gcggccatca cgtccgtcga aggcgcacgc ctacttgatc gttcgtctga
     2641 cctagaccat aaccgcacag tgctcacttt cgctggtccg cctgaagccg tggaagaagc
     2701 cgcgttccgc gccatccaga cggcctgtga gctgatcgac ctcaacaatc atacgggcga
     2761 gcatccgcgc atgggcgcga cggacgtggt tccctttgta ccgctcagtg gcacaaccat
     2821 ggaagagtgt gtggcgattg ccagacgttt aggtcaacgc gtgggcgagg aactgggcat
     2881 cccggtctat ttatatgagg cggcggcaac gcgtcccgaa cgcgccaatc tggagaacat
     2941 ccgcaaaggt caatatgagg gactcaggtc cgagatcgaa tccaacccgg accgtgcacc
     3001 tgatttcggt ccatcaaaga ttggaacagc gggggctacc gtgatcggtg cgcgcaatcc
     3061 gcttattgcg tttaatgtat atctcaccac cagtgatgta gatatcgcga aaaagatcgc
     3121 gaaggctgtt cgtcattcat ccggcggact tcgtaacgta aaagggctcg gattgcttgt
     3181 ggaagggcgt gcacaggtat caatgaatct tacagacttc cgcgagaccc cgatcgcccg
     3241 tgtggtcgaa acgattcgcc gtgaagctac tcggtacggc gtaggaattc atcacagcga
     3301 actggttggg ttgattccgc aagatgccct ggttgatgca gctgtttggt acacccagct
     3361 tgatggattc gacaaagagc agatccttga gacgcgcctc catgacgcgc ccgcctcacg
     3421 cttcgcgcaa accgacgata gtccgtcctt tattgaggag ttagccgccc cgacgccgac
     3481 tcccggaggc ggttccgccg ccgcgtatgc tgcagccatg ggagcggcgc tagtcgcgat
     3541 ggttgcggga gtcacgattg gtaagaagaa atacgcgcat gtagaggcca gcatgcaggc
     3601 tgtgcgcgtg atggcagaaa agctgcgtaa ggaattgact ttagccgtgg acgacgatgc
     3661 ttctgcgttt gaagctttga tggctaccta taaactcccg aaagagaccg aagaacaaca
     3721 aaagacgcgc gccgctcaaa tcaagagtgc gacattgact gctgcaacgg ttccactgca
     3781 cgttgcagag gacgcggtca aggtgatgga gattgcgttg aagtgtgcga aggatgcaaa
     3841 cgtcaacgcc attagcgacg ccatgtctgg ttttgccatg gcgcgtgccg cgctgactgc
     3901 ggctggctac aatgtgcgca tcaatattag cactctcgag gataaatctg tgggggataa
     3961 gatgctgaac gaactggctg agttggagaa gaagtctgaa aaggttgaaa aggaaattca
     4021 agccgtcatg aaggagcgcg gggggattta actctggagt ccaggagctt gctccggagg
     4081 atttgccaga aagatggcgg actccaaaat tctcttgtat gaagctgact gctttatttt
     4141 ttctgatctt ctttctaact tcctgtggca ccaccacaaa cgtaccagac atagcgacaa
     4201 acacaattgt cgttccttca ccgctacctc ccacatttac cgcaacgccg actgaactat
     4261 tacctaccgt tacccctcag cccacagcga cccagattcc gtgtgatcct tcttcagcag
     4321 acttttgtat tactgatggg cactttatct tccagcgtcc gataaagctg ctgacaaaca
     4381 cttctataga cccaacctat ccctacggct caactgccag agggacgcgt gatccgcatc
     4441 atggcgtgga gttccagagt ccgtttggca ctccagtcta tgctgccggt gatggtgtcg
     4501 tcctctttgc cggtgcagat gaaagggcga tttacagccc ctggccgaat ttttatggca
     4561 acctggtcgt tatcgagcat gagaacgatt tattcacctt atatgctcac ctctcgagaa
     4621 ttgacgttca aagtggaacg gttatccagg agggagataa actcggagaa gtcggtcaaa
     4681 gtggagcagc cacggggagt cacctccact ttgaggttcg ccgtggggtt gtggaagatt
     4741 acttttcgtc agtaaacccc gagctgtggc tcgcgccaac aaaaaatgaa aatggcgcaa
     4801 ttgcaatttc tgttgtcgat cagagtgcga agtttcagat tgcagagtta accatcagct
     4861 actaccctga tcggtctcaa ccacctgtcc tctcctatta tgcaactacg tatgcgcgtg
     4921 atctttccac aggggaagag aacgcagccg taagtgatgt gtccccggga aattatcgta
     4981 ttgcatttac aatgaatgga cgagtgtatg aacgatgggt tgaagtaaaa tcgggcagac
     5041 ttacgcaggt tgtgtttgtt gctaattaag gtgatagtca cttttgcaca gcactcccga
     5101 agtgaaacga agggagccat gtgactatca cctataccat caaattggag agaatatatg
     5161 ggactcaaac ctgatcactg gatccgcaag atggcaatgg aacaacggat gatcgagcct
     5221 ttcgtagata aacaagttcg tcagggagtg atttcttatg gcgtttcgtc gtatggttat
     5281 gacattcgcg tggcagacga gtttatgatc tttaccaacg ttcattctgc tattgtggac
     5341 cccaaaaact ttgacccgaa gtccatggtt gagttcaaag gggatgtatg tgtgatcccg
     5401 ccgaattcat ttgctctggc acgcaccatt gaatatttcc gcatcccgcg caaagtgctg
     5461 acggtttgtt tgggaaaatc cacatacgcc agatgtgggt tgatcgtgaa tgtgaccccc
     5521 ttcgagcccg aatgggaagg ttttgttacg ctggaaattt cgaataccac gccactgccc
     5581 gcgaagattt atgccaatga aggcctggcg caggtccttt tctttgaagc ggatgaggaa
     5641 tgtgaagtct cttatgccga taagaaaggg aaatatcaga agcaagagtc aattgtgctg
     5701 ccaaagctgt aaacaaaaaa agatactact acaatttatg cgcacctgaa ctgcttggct
     5761 ttgtaattaa cccttgagac tgaatccctt gtttcataaa aacgtctatt aattcttctt
     5821 ttgcgttttc agccttttct cttgaaacaa aacaaataat agaaggccca gcgcccgaaa
     5881 gcgcagccgc gcccaattgc ctggcggctt catacgcagc caggcctccg gcgatgtgct
     5941 ttagccgata tggctggtga atgcgatcat ccatcacttt ttgaagcaaa tccaaatcgc
     6001 cgtttcgtag ggtatcgatc acaagtgcag tgcgtccaat gttgtaaatc gcatccgcac
     6061 gtgatactgt atctggcaag accgatcgcg ccgttcgcgt ttgccattgc acgtctggct
     6121 tgacgatcac aatcgccagc gctggtattt cgtatcgccg ggtgatgatc tcacggtcgg
     6181 taactacgga aaccaccagc ccgcccagga gcgcaggtgc aacattatct ggatgtcctt
     6241 ctatctcggt agcaagtttg accaatgcat aaatatccaa tggggcgccg aggatttcgt
     6301 tcgccccata aatcccggcc acgatggccg ctgcactgga gcctaaccca ctgctatgca
     6361 ggatattgtt gtgtgcctga atatgtattc catgcagttc tttgccgcag acctcgtaca
     6421 cttttttgaa cgagtctgtc agcagattga tgggacgatg attcagctta tccgcgcctt
     6481 cacccgtaac ctggtacgta atggaatcag attcttcaaa agacacctca ttccagatat
     6541 gcagcgcgag tccaagacaa tcaaagcccg gccccagatt cgccgaggtg gcaggcacac
     6601 ggatttttat cacggatgaa attataatcg cctcaattct gacattggag ttaacatgac
     6661 gtaccaggga attatccacc gttacagaaa tttacttgac ttgcccgcgc attcgcagac
     6721 ggtttcattg cttgaaggcg ataccccatt aatccccatg ccgcgtttaa gtaaacagtt
     6781 tggttgcgaa ctgtatgtga agtttgaggg gttaaacccg accggttcat tcaaagatcg
     6841 tgggatgact gcggcgatca gtgaagcagc tggccgcggt gcccaaaccg tcatctgcgc
     6901 ttcgactgga aacacagccg catctgcagc agcttatgca gcccgtgcgg gattgcgcgc
     6961 catcgtcttg atcccggatg gcaaagttgc ggtcggtaag ctcgcgggtg cagtcgcgta
     7021 tggtgcacaa gtgattcaag tgcaaggttc ttttgatgat gcgctttcgc tggtcgttga
     7081 aatcactcaa aagactccaa ttgcactggt caattcaatc aacccatttc gaatcgaagg
     7141 acaaaaaaca gccgcattcg agatttgcga tactctctca cgtgcacctg attggttatg
     7201 tttaccagtc ggcaatgcag gcaacattac ttcctattgg gcgggcttca aacaatatca
     7261 aaaaggattg ccgcaactgc tgggcgttca ggcagaagga gcggctccgc tggtgttggg
     7321 tcatccggtc gataaacccg aaacgatcgc taccgcgatt cgcatcggac gaccggcgcg
     7381 cggtgaatat gctttggaag ccgcacaaga gtcaaaaggc aaacttatcg cagtctctga
     7441 tgcggaaata ttatccgctc agaagatatt agcaggggag ggagtttggg tggagcccgc
     7501 ctcggcggca gggctggcgg gtctcattgc ggaaacagaa aaggggtcgc tcgatgtgaa
     7561 gggaaaaaga gtggt
//
LOCUS       DASTJP010000284         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1011021, whole genome shotgun sequence.
ACCESSION   DASTJP010000284 DASTJP010000000
VERSION     DASTJP010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011021"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..997)
                     /locus_tag="VFQ23_08570"
     CDS             complement(125..997)
                     /locus_tag="VFQ23_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9906682.1"
                     /translation="MLTSFSNWLKDHPIPAFFALTFLIAWSIWLPLGIFAPGQVLLTL
                     VGAWSPTISAILLIGLSDGKGGVRRFLQKVLHWRVGIHWYVVVLFGTAGIAYLAIGIG
                     NVLGIPIPQISLPQGLPQEALFGVLPILFLINIFLGGPLAEDIGWRGYILPKLREQMS
                     TLQASLLIGIIWAIWHLPFFIFPEWNGAVGNMPFLVFTLLTTSWSVLFAWVYVNTESI
                     LMPVLFHAAINTTLGTLGVLGQADGDVRSLIINTALTWSVAGIVVAVFGSDLKSTRRD
                     RMAKENMDSSFRHV"
     gene            complement(1069..1266)
                     /locus_tag="VFQ23_08575"
     CDS             complement(1069..1266)
                     /locus_tag="VFQ23_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906683.1"
                     /translation="MNNNNLLARTVIIFVTLAVAGIGLTTGARFLMDAVAQNILIAVG
                     SAIFGASLAFFLIRFFSLIEK"
     gene            complement(1365..1979)
                     /locus_tag="VFQ23_08580"
     CDS             complement(1365..1979)
                     /locus_tag="VFQ23_08580"
                     /inference="COORDINATES: protein motif:HMM:NF019960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9906684.1"
                     /translation="MRRTKEDAAVTRKTLLKTALSVFSAKGYAAATLDDVAKAAKVTR
                     GAIYWHFKSKADLFNTLVQEVSARGAAVVQQAVAEGGTLIEILHRIFVRQCALIEEDK
                     EARAVMELALFKTGLSPELQPGRKKQLEAGNALVEGIAAAMQQGIKQGVLRNDISPVD
                     MARGFLAFENGAIQLWLASPRSFSLKDSAPSFANILMAGLQKQK"
     gene            2218..2739
                     /locus_tag="VFQ23_08585"
     CDS             2218..2739
                     /locus_tag="VFQ23_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9906685.1"
                     /translation="MFKLTTARLFLRHFHILDDAAMYHIFGDAEVMRFGDGIQTKAWV
                     QDWLRTCLERYYQTWGFGPYAVVEQSTHNVVGYCGLFYFPDIDGQPEIEIGYRLARSA
                     WGQGYATEAALSVRDFAFNTLSIKRLIAMIDPSNRASIRVAEKLGMQYEKEIMFENYS
                     HPDHIYAVNSMER"
     gene            complement(2771..>3277)
                     /gene="holA"
                     /locus_tag="VFQ23_08590"
     CDS             complement(2771..>3277)
                     /gene="holA"
                     /locus_tag="VFQ23_08590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET9906686.1"
                     /translation="AAEMLKDMVGVDTRQAGMEIAKLLAYANWARPVNSQDVEAVCIV
                     TSQQSVFDFVDALSNGNGKAAQHLLHRLLENEDPFLLWGMVVRQFRLLIQAREILDAG
                     GNQNDTARVLGVHPFVAEKTTQQAMRFSIESLESIYRELLRIDEGVKTGHLTLELAMD
                     TLVVELTK"
BASE COUNT          876 a          860 c          761 g          780 t
ORIGIN      
        1 aagctggagt ggaatgaaac atcgccatga agcgatagag tccatttgaa gatgtatcga
       61 tagagctttg gcaaaattcc aggatgagta aggattagat aaaaccatat aggtatgcct
      121 ggaatcaaac atggcgaaag cttgaatcca tattctcttt cgccatccta tcccggcgtg
      181 ttgacttcaa gtcgcttcca aaaactgcga cgacaattcc cgccactgac caggtcaacg
      241 ctgtgttgat aattaacgat cgtacatcac cgtcggcttg acccagcacg cccagcgttc
      301 ccagagtagt gttgatcgca gcatggaata ataccggcat caaaatgctc tcggtattaa
      361 catataccca ggcaaacaat accgaccaac ttgttgtcag cagggtaaac actaagaaag
      421 gcatatttcc tactgcacca ttccattccg gaaagataaa gaaggggaga tgccagattg
      481 cccaaatgat cccaatcaaa aggctggcct gcagcgtact catctgttca cgcagtttgg
      541 gcagaatata gcctcgccag ccaatgtctt cggcgagcgg tccgcccagg aagatgttga
      601 tcaaaaagag aatcggtaac acaccaaaca gagcctcctg cggtaatccc tgcggaaggg
      661 aaatctgggg gataggaatc ccaagcacgt taccaatacc gatcgcgaga taagcgatgc
      721 ctgcggttcc aaacaaaaca accacatacc agtggatgcc aacgcgccag tgcaatactt
      781 tttgcagaaa ccgccgaaca cctcccttcc catccgaaag tccgatcaac aggatcgcgg
      841 aaatcgtggg tgaccaggct ccaactaacg taagcagcac ttgtcccggt gcaaagatgc
      901 cgagcggcag ccagattgac caggcgatga ggaaggtcaa tgcaaagaaa gcagggatgg
      961 gatggtcttt gagccaattt gagaaggaag ttaacatctt gataccctga aggtcggcgc
     1021 ttcctgttcc cctcaagaaa ggaagggaac aggagaagcg acttttgatt atttctcaat
     1081 cagcgagaag aatcgaatca gaaaaaacgc aagtgacgcg ccgaagattg cgctgccaac
     1141 agcaatgagg atattttgcg caacggcatc cattagaaat cttgcacctg tggtcagccc
     1201 tatgcctgct acggcaaggg taacgaagat aatcacggtg cgggcaagca ggttattgtt
     1261 gttcattttg tctcctttat atatttacat actctcatgt atgtaaactg ggtaaaaaaa
     1321 tttatcttac gataccgaca ttgactactt ccacaaccaa cgtcttactt ttgtttctgt
     1381 aaaccagcca tcaggatatt agcaaaggat ggagcgctat ccttcaaaga gaaggacctg
     1441 ggggaagcaa gccacaattg aatcgcacca ttttcaaatg ccagaaaccc tcgtgccata
     1501 tcaaccgggc taatatcatt gcgcaacaca ccctgtttga tcccctgctg catcgcggcg
     1561 gcgatccctt cgaccagcgc attgcccgcc tcaagttgct ttttgcggcc aggctgcagt
     1621 tccggcgaca atcctgtttt gaacaaagcc aattccataa cggcccgcgc ttctttatct
     1681 tcttcgatca acgcgcactg acggacaaag atccggtgaa gaatctcaat cagcgtccca
     1741 ccctccgcca cagcctgttg cacaactgcc gcgccacgtg ccgaaacttc ctggaccagc
     1801 gtgttgaaca gatcagcctt gctcttgaag tgccagtaga ttgcgcctcg agtcactttg
     1861 gcggctttgg ccacatcgtc cagcgtcgcg gccgcatacc cttttgcact gaaaacggat
     1921 agcgcagtct tcaacagtgt tttgcgggtg accgccgcgt cttcctttgt ccgtcgcata
     1981 atatacatac tatcgcatat gtatattttt gtcaaggcag attttgtgcg atgggagtgg
     2041 ctaagagagt gagtgaaact ctacttagcc acagagagca ctgagccctt tgaccccgca
     2101 cctgcgctgc gttcgaggca ggtggaaaaa aaactcaaaa actctgtggg ctctgtggct
     2161 tacacactaa attcgccact cccttgttcg atgcgctata cttgatttac gctccacatg
     2221 tttaaattga cgaccgccag gcttttcctt cgacactttc acattctgga tgacgcagcg
     2281 atgtatcaca tcttcgggga tgcagaagta atgcgtttcg gcgatgggat ccaaaccaaa
     2341 gcatgggtac aagactggtt gcgtacatgc cttgaacgct actatcaaac gtggggtttt
     2401 ggaccgtatg ctgtggtcga gcagagtacg cataacgtcg ttggatattg tggtttgttc
     2461 tattttccgg atatcgacgg tcagccagaa attgagatcg gttatcgcct ggcacgctca
     2521 gcctggggac aagggtacgc tacagaggct gccctgagcg tacgagactt tgcttttaat
     2581 acactgagca tcaagcgtct catcgccatg attgacccgt ctaaccgcgc ttctattcgg
     2641 gtggcagaga agctcggtat gcaatacgaa aaggaaatta tgttcgagaa ttattcccac
     2701 cctgatcata tttacgctgt gaattcgatg gaacgttagg agaaatcctg ccctttaaaa
     2761 tcacgctgcg ctatttcgtc agctccacca ccaaggtatc cattgccagc tccagagtga
     2821 ggtggcctgt ttttacccct tcatcgattc tcaacaattc acgatatatg ctttcgagtg
     2881 attcgattga aaagcgcatt gcctgttgcg tggtcttttc tgcgacaaaa ggatgcacac
     2941 ccaatacgcg cgccgtatca ttttgattgc cgccagcgtc aaggatttca cgcgcctgga
     3001 tcaacaaacg gaactgcctc acaaccatcc cccacaacaa aaacgggtct tcattttcga
     3061 gcaggcgatg caataaatgc tgcgccgctt taccattgcc attggacaag gcatccacaa
     3121 aatcaaatac cgattgctgt gatgtgacaa tacagacagc ttccacatcc tgactgttga
     3181 ccggccttgc ccaattggca taagccagca gtttggcgat ctccatcccc gcctgacgcg
     3241 tatccacacc gaccatgtcc ttgagcatct cagccgc
//
LOCUS       DASTJP010000285         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_986579, whole genome shotgun sequence.
ACCESSION   DASTJP010000285 DASTJP010000000
VERSION     DASTJP010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986579"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFQ23_08595"
     CDS             <1..213
                     /locus_tag="VFQ23_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906687.1"
                     /translation="QSARFTSMSRRVASSILCSQAESMPPQRHALGQAFKVILDTFAP
                     GKWTINKTMRNFFNTIYRACVYTVDY"
     gene            236..1081
                     /locus_tag="VFQ23_08600"
     CDS             236..1081
                     /locus_tag="VFQ23_08600"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9906688.1"
                     /translation="MLHQPIYAPVPMLAADVSCFWALEQDQESYNREVYLPDAYIEVI
                     INVGAPLVLESEHGMLELPRAFVNPLQSKPLRIRAAGFCQMISMQLYPWAVKPILNID
                     PDPSTVHVIGLDADWQRFADDLTQIVVHQGYGEAIDCYQEYVCKTAYRHKHDVTLLRT
                     AGQVLHRSHGQIRMADLAAQSYLSSSQFERQFKHYTAVSPKAYARIVRFGSVHSALLV
                     NPFIRLVDLADVYGYSDQAHLIREFKSFAGCTPHDFAASAPAYFELRQNEDWSDFMKQ
                     YFDLS"
     gene            1242..1835
                     /locus_tag="VFQ23_08605"
     CDS             1242..1835
                     /locus_tag="VFQ23_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9906689.1"
                     /translation="MIDFITTLDGYASGEGWPGWWGLEGPEYLAWLGEQPAATYLMGA
                     NTYRLMYGFASGEPPPGAAEMEAEEEASVDELTRTPKVVFSASLEEPITWANTTLVRG
                     DAVQAVRAMKADGSGLLSTLGSVSLSRSLLRAGLVDRFRVVMFPVITGATGSERIYDG
                     YPDVALEMIDHRTFDGRIQLVEYRPRVLDGPPLAPTA"
     gene            1931..2512
                     /locus_tag="VFQ23_08610"
     CDS             1931..2512
                     /locus_tag="VFQ23_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906690.1"
                     /translation="MGGCDGREGVVYVSEIGSALRIGRRVVVTGMAGAGKSTFAQQLS
                     AKTSLPVIHLDVHFWKPGWVEPSDREWRDVQSTLLAGEEWIVDGNYHETLDLRLERAD
                     TVVYLDTPWWLCAARAFVRGLRRPEGSQLPDGCEDTGWRRLRDEWWLMWRVWLGRRSE
                     RERELAIVARHGSHAALHVLRSKRAVRKFIDHV"
     gene            2812..3318
                     /locus_tag="VFQ23_08615"
     CDS             2812..3318
                     /locus_tag="VFQ23_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9906691.1"
                     /translation="MQVDWRASTWNQFGAAIDMFSDTIQLCPDHLWSTVVYIDPEDER
                     YGQFWFIAYHTLRWLDLFLNGTKEGFKPPSPFIVGALPDQPYTKDQILVYLDACREKC
                     QSTIEALTDERAQQRCVFEWMEPSFLELQLYCMRHVQEHAAQLSLVLGQHGVTGMDWI
                     AIARNKNT"
     gene            3398..>4302
                     /locus_tag="VFQ23_08620"
     CDS             3398..>4302
                     /locus_tag="VFQ23_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9906692.1"
                     /translation="MNSNYPSGTVTFLFTDIQGSTRLWQEKPQAMSVSHDRHDTILRV
                     AIESNHGYIFRIAGDSFSAAFHNSIDGLRATVAAQRGLQAETWGETGAIKVRMGLHTG
                     TAEILADGKYEGYATIASTQRVMSAAHGGQTLLTQTTYDLLQNELPDDVSLHDLGEHR
                     LKDLRAPLRLYQVNAPDLQQDFPPIKSLDRQPNNLPAQLTSFVGREKEIDEIKSAISA
                     SRLVTLTGSGGTGKTRLSIETGMQLLPKFSNGVWLIELAPLSDESQIIPALAQAFGLQ
                     ELPFNPLKNIVMDYLRDKKLLLILD"
BASE COUNT          978 a         1131 c         1198 g          995 t
ORIGIN      
        1 caatctgcgc gatttacaag catgtcgcga cgcgtggctt cgagtatcct ctgctcccaa
       61 gcagagtcca tgccgcccca gcgccacgcg ttagggcagg catttaaggt tattctggat
      121 acgttcgccc caggaaaatg gacaatcaac aaaacgatgc ggaatttttt caatacaatc
      181 tatcgcgctt gcgtttatac tgtagattac tgagtcacta tagtgagagt gtttaatgtt
      241 gcatcaaccg atatatgctc cggtgccaat gctggcagca gatgtgagct gcttttgggc
      301 acttgagcaa gaccaggaaa gctataaccg tgaggtgtac ctacccgatg cctacattga
      361 agtgataatc aatgtgggtg cgccactcgt gttggagagc gaacacggaa tgctcgaact
      421 accgcgggca tttgtaaacc cgctgcaatc taagccgctg cggattcgcg cggctggttt
      481 ctgccagatg atttcgatgc agttgtatcc ctgggcggtc aaaccgatct tgaatattga
      541 tcctgatcca tcaactgtgc atgtgatcgg cctggatgca gattggcagc gctttgcgga
      601 tgatctgaca cagattgtgg tgcatcaagg ctatggagaa gccatcgact gttaccagga
      661 gtatgtctgc aaaactgctt atcggcataa gcacgacgtc acgctactcc gcactgccgg
      721 acaggtactg catcgctctc atggtcaaat ccgcatggca gacctggccg cacagagcta
      781 tctgtcgtcc agccaattcg agcgacagtt caaacattac acggctgttt cgcctaaagc
      841 ctatgcccgg attgttcgct ttggtagcgt ccattctgca ttactcgtga atccattcat
      901 ccgattagtg gatctggcgg atgtttatgg ctacagtgac caggcgcacc tgatccgcga
      961 attcaagtct tttgcaggct gtacaccaca cgatttcgct gcttctgcac ccgcctattt
     1021 tgaacttcgg caaaatgaag attggtccga tttcatgaag cagtattttg acttatctta
     1081 ggcagggtaa gtgaataaag caatctcaat aatgtagtga tctccgaaac ctggatatga
     1141 gagaaaggaa gaaccatgaa actggtaacc aatcacgttc taacggcaac atcatccgac
     1201 gaccaatcca tcgctcgagg aggagcacga tgcgagagct tatgatcgac ttcatcacca
     1261 ccctggacgg ctacgcgtcg ggggagggct ggcccggctg gtggggtctc gaaggaccag
     1321 agtacctcgc ctggctcggc gagcaaccag cggcgacgta cctgatgggc gcgaacacgt
     1381 accggctgat gtatggcttc gccagtggag agccgccgcc aggagccgcc gagatggagg
     1441 ctgaggaaga ggccagcgtc gacgagctca cgcgaacgcc gaaggtggta ttctcggcgt
     1501 cgctcgagga gcccatcaca tgggccaata ccacgctcgt acgcggcgac gccgtgcagg
     1561 cggtccgggc gatgaaagcg gatggctctg gcctcctcag cacactgggc agcgttagcc
     1621 tgtcccgctc actgctgcgc gccgggctcg tcgatcgctt ccgggtcgtg atgttccccg
     1681 tgatcaccgg tgccactggc tcggaacgca tctacgacgg ctatcccgac gtcgcgctcg
     1741 agatgatcga ccaccgcacc ttcgacggcc gcatccagct cgtcgagtac cggccgcgcg
     1801 tgcttgacgg cccgcccctc gcgccgactg cctgaggctg agcgctgctg gaaaaaaccg
     1861 ctcatttgtg cgttgagctg ttgaccggcg ctgcccggtc aggacttgat cgcagcttaa
     1921 gcgccattcg ttgggcggct gtgatggaag agaaggagtt gtttacgtga gcgagatcgg
     1981 ttcagcgctg cgcattggac gacgcgttgt cgttactgga atggctggtg cgggcaagag
     2041 cacatttgca caacagctat cggcgaagac cagccttccg gtgattcatc tggatgtcca
     2101 cttctggaaa ccgggctggg tggagccgtc tgatagggag tggcgtgatg tccagtccac
     2161 gttgctcgct ggcgaggagt ggattgtcga cggcaactat cacgagacgc tggatctgcg
     2221 acttgagcgc gcggatacgg tcgtgtacct cgacacgcca tggtggctct gcgcagcgcg
     2281 agcgttcgtg cgtgggttac gtcgtccgga gggttctcag ttgcctgacg gctgcgagga
     2341 tacgggatgg cgacggttgc gtgatgaatg gtggttgatg tggcgcgtct ggttggggcg
     2401 gaggtcagag cgcgagcgag agctggcgat cgtggcgcgg catggaagcc atgcggcgtt
     2461 gcacgtttta cgctccaaac gtgccgtccg aaagtttatt gaccacgttt agctgttcct
     2521 accgagactc attccatcgc actcgcgatc agtacgcact tggatggggc ttgtataata
     2581 gactgtcaac acaccctgtg gcacggactt ctcaagctgc ggcccaacac agcccccatc
     2641 gctgcgctta ggggatcgtc gacaggactg gacggtgggg tacggtgcgg cttttttgga
     2701 gagtttcaca accccaccga gcctttaacg taaaccgtcg gtccctggag ctattgcaag
     2761 ccatagaaat gcaacttttt taaagataca acttagtaca ggaggcacta gatgcaggtc
     2821 gactggagag caagtacctg gaaccaattt ggggctgcca tcgatatgtt cagtgatacg
     2881 atacagttat gccctgatca tctctggagc accgtagtgt atatcgatcc ggaggatgaa
     2941 cggtatggtc agttctggtt tattgcctac cacacactgc gctggctaga cctctttctc
     3001 aatggcacca aggaaggttt caagccgccg tcccctttta tcgttggggc gttgccggat
     3061 caaccctata caaaagatca gatccttgtt tatctggatg cctgccgcga gaagtgtcag
     3121 tcgaccatcg aagcactgac agacgagcga gcgcagcaac gttgcgtctt cgagtggatg
     3181 gagcccagtt tcctggaact gcaactgtac tgtatgcgac atgttcagga acacgctgca
     3241 cagttgagtt tggtgcttgg tcaacatggc gttacgggca tggattggat cgccattgcg
     3301 agaaacaaaa atacatagct atatggcaaa aatcgacaat tgattttgta acagagagcg
     3361 tgattgagag ttgatgcccc ttgcctcaat tgtagaaatg aattcaaatt acccttcagg
     3421 caccgtcact ttccttttta ctgatatcca gggcagcaca cgcttatggc aggaaaagcc
     3481 acaagcaatg tccgtctcgc atgatcgtca tgacacgatc ttgcgtgtgg cgatcgaatc
     3541 aaatcacggc tatatctttc gaattgccgg cgattcgttc agcgccgcat ttcataattc
     3601 aattgacgga ttgcgtgcga ccgtcgcggc gcaacgtggt ttacaagctg aaacatgggg
     3661 cgagaccggc gcgatcaaag tccgcatggg gttgcatacg ggcacagcag aaatattggc
     3721 agatggcaaa tatgaaggct acgctaccat cgcatcaacg cagcgcgtga tgtctgccgc
     3781 acatggcggt cagactctcc ttacacaaac cacgtatgac ttattgcaaa atgaattgcc
     3841 cgacgatgtc tccttgcacg atctgggtga acatcgtctc aaggatttgc gcgcaccgct
     3901 gcgactgtat caggtcaatg caccagattt gcaacaggat ttccctccga tcaaatcgct
     3961 tgatcgccaa cccaacaacc tcccggctca actcacctcc tttgttggtc gcgaaaaaga
     4021 gatcgatgag attaagtcag caatcagcgc gtcgcggctc gtgactctca ccggttccgg
     4081 cggcacaggc aagactcgtc tttcaattga aacaggtatg caactgctac caaaattctc
     4141 gaacggtgtc tggctcattg aactggcacc gctcagcgat gagtcgcaga tcatccctgc
     4201 cttggcgcag gcgtttggtt tgcaagagtt gccgtttaat cctctgaaaa atatagtcat
     4261 ggattatctg cgcgataaaa agctcctgct cattctcgat aa
//
LOCUS       DASTJP010000286         5721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_238393, whole genome shotgun sequence.
ACCESSION   DASTJP010000286 DASTJP010000000
VERSION     DASTJP010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238393"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..468)
                     /locus_tag="VFQ23_08625"
     CDS             complement(19..468)
                     /locus_tag="VFQ23_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906693.1"
                     /translation="MKTRTQCFMIAGLVILILAVGWLGYSTRNQEPAQVFSATINRDC
                     APWDGGAFTVSIPVDVTTTIMISIWQSPDIQHPIAFTFPDESGQTGFVYILPEIDPLQ
                     PLSGEVFFGRVEQGLPVEGEFNLTTERGEQFNGRFKAVWGDQVVYCG"
     gene            complement(637..3612)
                     /locus_tag="VFQ23_08630"
     CDS             complement(637..3612)
                     /locus_tag="VFQ23_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9906694.1"
                     /translation="MTSQKPGMPLRTALFYALFAGLWILFSDQILLLFVDDLSQHSTL
                     QTFKGWFFVVVSALLLYIHLQRELNAQRERSKAFRSNAQQLQLVYESISDVIFLLSVE
                     PNGRYRFVSVNNAFLRTTGLESEQVIGRYADEVLPASAQKLVFSNYKKAIEERSPVTW
                     EDVSVYPSGTTTAVITVNAIFDEAGNCTHLVGGVHDLTELKRVEQELRASNERFQQLA
                     ENISEVFWITDPQLKKDLYLSPAYEEIWGRPVQGQLENVETFIECVVPEDRGMVLLTI
                     EKQERGEKTEMEYRIVRPDGSIRWIWDRAFPILNERGQVIRVAGIASDITERKDAEIE
                     RRALLEIAEGTASSKDLHELLGLIHRSIARVIFAENFFVILHNPKTDLFEEVYSVDQY
                     DPPSLPFKLEKSISAYVYRTGEPLLVTQERFDELVKQGEMELIGTNSPSWLGVPLKVS
                     GETIGVMVVQDYDRADRYSERDRDFLFSIASQIALTVQRRQAEEALKNSESRFRALIE
                     NGLDNISLLAVDGTLLWESPATVRMLGYQPDEFVGRDMFSLVHPDDLDWMREIYGEIL
                     QAPGSRQHRSFRLRHADGTWRWAEAIVTNLLNEPSVNALVVNYRDVTDRKQAEMEITR
                     LLAESQRRLKQLEALHSIDLAISASMDLRTTLSVLIQQVESLLNVDAVDILLFNPDLQ
                     QFTFSAGRGFLSDEIQRVRIRYGKSFAGRVVFERKRVLVSGEHAMQAEPAFSDIYERE
                     GFLAYAGVPLMAKGQIKGVMEVYHRSPFSPDPEWLNFFETLAGQAAIAVESAQLLDGL
                     QRSNIELAFAYDQTIAGWSHAMDLRDKETEGHTQRVTDLTLKLARVMNFNESQLIHVQ
                     RGALLHDIGKMGVPDNILLKADKLTAEEWEKMRQHPEFAYQMLASIQYLKPALDIPYC
                     HHEKWDGTGYPRALKGEEIPLAARIFAVVDVWDAVTNDRPYRAAWTQAQALEYIREQS
                     GKHFDPQVVDIFLRLMASS"
     gene            complement(3857..4240)
                     /locus_tag="VFQ23_08635"
     CDS             complement(3857..4240)
                     /locus_tag="VFQ23_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9906695.1"
                     /translation="MMTSKPKTNQTNIPKKRNNHASYLISLFDNISDGLIATDLEFNI
                     LEWNAAAEAMYGWTDGYPRDLKGEEIPPAARIFTVADVCDALTSDRPYRGAWSRSQAL
                     KHIEEQSGKQFDSQAVRAFQNLIKE"
     gene            4443..5243
                     /locus_tag="VFQ23_08640"
     CDS             4443..5243
                     /locus_tag="VFQ23_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906696.1"
                     /translation="MDKSLSQFSKLVLLGAGALAVIVGPILYFFPEDTGTYFAWSIQH
                     PLTPVYMGASYLAGIGNVLAVRANKWSLARVQLPAIIFFTLTMLVATLMHIEIFNWSH
                     PVAWGWLAVYVISPVAAVLVFLQMERGFQSPQLVSNKLPASFSPVMLIFAAIYAAIGL
                     ALFFFPEQTASLWAWSLTPLTARVIGGWCISAAALQYMLAQQKTLHTARVGIVANFLI
                     TSLHLIGAFLHFSEFNGSQISIWLYLLLNLSVNGFSIYSWVQSRAHTK"
     gene            complement(5270..5677)
                     /locus_tag="VFQ23_08645"
     CDS             complement(5270..5677)
                     /locus_tag="VFQ23_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906697.1"
                     /translation="MPTSLTPPPQDDEVECGNCGAYIYHGLLKCPNCGVYLEDPVDTE
                     LNEPPRFRPKSKLALMIESIVRKIRGEPDYAEELFTGVLREKALFDDLLRKVGGDRSA
                     VERLIEYERGKTPNATRLTCLQNAISRWEQENK"
BASE COUNT         1370 a         1485 c         1350 g         1516 t
ORIGIN      
        1 gtaaatattg tgaggacgct acccgcaata caccacttga tctccccaca ccgctttgaa
       61 cctcccattg aattgttcac ctcgttctgt cgtgaggttg aactcgcctt cgacgggcag
      121 cccctgctca acgcgcccga agaaaacctc cccgctcaat ggttgaagtg gatcaatttc
      181 tggcagaatg taaacaaagc cagtttgtcc agattcatcg gggaatgtaa atgcgatcgg
      241 gtgttgaata tctggcgatt gccaaatgga aatcataatc gttgtagtta catcaacggg
      301 aatcgagacc gtaaacgcgc ctccatccca cggtgcgcag tcacgattta tagtggctga
      361 aaagacttgt gcgggctctt gatttcgcgt tgaatagcca agccagccga cagcgagaat
      421 aagaatgacc agaccagcga tcataaaaca ctgtgttcga gttttcatgc tgaaagcagg
      481 acgctgagtt tattttgaat gttcctccta gaaacgtgtt cgcgctgagc ggaacgagag
      541 cgtgattgtc gcccgaacgc agacgaagcg aaggttttga atatattgcg tctcgtctac
      601 gtgatgctgg gcatcttctc cgctcgacgc gagatatcag gaactggcca tcaaccgcag
      661 gaatatatcg accacctgtg gatcgaaatg ttttccggat tgttccctga tatattccaa
      721 agcctgtgcc tgggtccagg cggcgcggta tggacggtca ttggtgactg catcccaaac
      781 atcaaccaca gcaaatattc gcgccgcaag tgggatttcc tctcccttca gggcgcgcgg
      841 gtatcctgtt ccatcccatt tttcgtgatg acaatatggg atatccaacg caggtttcag
      901 gtactggatc gaagcaagca tctgataagc aaattccggg tgctgacgca ttttctccca
      961 ttcttcggca gttaacttat cagccttgag caggatgtta tctggtacgc ccattttgcc
     1021 gatatcatgc aacagcgcgc cgcgctggac atggatcaat tgagattcat tgaagttcat
     1081 cacgcgcgcc agtttcaggg tcagatccgt gacgcgctgg gtatgtcctt ccgtttcctt
     1141 gtcccgtaag tccatggcgt gtgaccagcc agcaatcgtc tgatcgtagg caaaggcaag
     1201 ctcgatattt gacctctgca atccatccaa tagctgcgca ctttccacgg cgatggctgc
     1261 ctgcccagcc agggtttcaa aaaaattcag ccattcgggg tcagggctaa aaggcgaacg
     1321 atgatacacc tccatcacac ctttgatctg gcctttcgcc atgagaggta cgcccgcata
     1381 cgccaggaag ccttcccttt catagatatc tgaaaaggct ggctcggcct gcattgcatg
     1441 ttcaccagag acgagtaccc tcttacgctc aaaaacaaca cgcccggcaa agctctttcc
     1501 ataacgaatc cggacgcgtt ggatctcatc ggagaggaaa ccgcgacccg ccgagaaggt
     1561 gaactgctgg aggtctgggt tgaacagcaa gatatccacc gcatccacat tcagcagaga
     1621 ttccacctgc tggatcagga cacttaatgt ggtacgtaaa tccatgctgg cactgatcgc
     1681 gagatcaata gagtgcaatg cttccaactg tttcaggcgt cgttgagatt ctgccaacag
     1741 gcgggtgatc tccatttctg cctgtttgcg atcggtgacg tcgcggtaat tcaccaccag
     1801 cgcattcacg ctgggttcat tcaacaggtt ggtcacgatc gcttccgccc agcgccaggt
     1861 gccatcggcg tgccgcaggc gaaatgaccg gtgctggcga ctgccgggtg cttgaaggat
     1921 ttccccataa atttcgcgca tccaatccag atcatccgga tggactaatg agaacatgtc
     1981 gcggcccaca aactcatcgg gctgataccc caacatacgg acggtggccg ggctttccca
     2041 cagcagcgta ccatccacag ccaggagtga aatgttatcg agcccgtttt cgatcaacgc
     2101 acgaaagcga ctctcactgt ttttcagggc ctcttcggcc tgcctgcgtt gaactgtgag
     2161 agcgatctgg ctcgcaatgg aaaacagaaa atcccggtca cgttccgaat aacggtccgc
     2221 gcggtcgtaa tcctgtacca ccatcacgcc gatcgtctcg cccgagactt tcaggggcac
     2281 cccaagccag gagggtgagt tggttccgat cagttccatt tcgccctgct tcaccaactc
     2341 atcaaagcgt tcctgggtaa cgagtaatgg ttcgcccgtg cggtagacgt aggcgctgat
     2401 gctcttttcc aatttgaagg gaagagaagg cgggtcatat tgatctacgg aatagacttc
     2461 ttcaaacaga tcagttttgg ggttatgaag gatgacaaag aaattctctg caaaaatcac
     2521 tctggcaatc gagcgatgga tcagacccag tagttcgtgc aggtctttgg aactggctgt
     2581 tccttctgca atttccagca gggcccgacg ttcgatctcc gcatctttac gttctgtgat
     2641 gtctgaggca atcccagcca cacgaatgac ttgacctctc tcattcagaa ttggaaaggc
     2701 tcggtcccat atccagcgaa tggatccatc ggggcgtaca atacgatatt ccatttccgt
     2761 cttttcgcca cgctcttgct tttcgatggt cagcaatacc atccctcgat cttccggaac
     2821 tacacattca ataaacgttt cgacattctc aagttgtcct tgaaccgggc gcccccagat
     2881 ctcttcgtag gccgggctga gatacaggtc ctttttgagt tgaggatctg tgatccaaaa
     2941 tacctccgaa atattttcgg caagctgctg aaagcgttca ttcgaagcgc gcaattcctg
     3001 ctcgactctc ttcagttccg tgaggtcgtg aacccctccc actagatgcg tgcaattgcc
     3061 tgcttcgtca aagattgcgt ttacggtgat aacggctgtc gtcgtgccgg agggatacac
     3121 cgagacatct tcccacgtca ccggacttct ttcctcgatc gcttttttgt agtttgaaaa
     3181 caccaacttc tgtgcagaag caggaagtac ctcatctgca tatcttccga ttacctgttc
     3241 gctttccaga cccgtcgtcc gcaggaaagc attattgacc gacacaaatc gataacggcc
     3301 atttggttcc accgagagga ggaaaatgac atccgaaata ctctcgtata caagctggag
     3361 ttgctgcgca ttgcttcgaa acgccttgct tctttctctt tgagcattca attccctctg
     3421 caaatgaatg tacaataaaa gagcactaac aacaacaaaa aaccagcctt tgaaagtctg
     3481 taaagtagaa tgctgagata gatcgtcgac aaataacaac aggatttgat ctgaaaacag
     3541 aatccataat cctgcgaaca gcgcataaaa aagagcagtt cgaagtggca ttcccggttt
     3601 ttgggaggtc atttttactc tagaagatct gcttccgata tttcatcatc ccgttagtta
     3661 tttggttaag tatatccatc tgatgtattc tattgtagca aaaactcatt gggttgaaat
     3721 cgtaccccat ggtactcaag ctccttgaaa aataaataca catccccaat catagcatta
     3781 ttggttcgga aagagattac aaacgattat ttgctcacaa aatgtaatcc ttttttagta
     3841 gtggcagatc gaatagctac tctttaatta gattttgaaa cgctctcacc gcctgggaat
     3901 cgaactgttt tcccgattgc tcctcaatat gttttaatgc ctgcgatctg ctccatgcgc
     3961 ctcgataagg ccgatcactc gtcagcgcat cacatacatc cgctacggta aagattcgtg
     4021 cagccggtgg aatttcttct cccttcaaat cgcgtgggta tccgtctgtc catccgtaca
     4081 tggcttcggc cgcggcgttc cattccagga tattaaattc cagatcggtt gcaattaaac
     4141 cgtctgagat gttgtcgaaa aggctaataa gataggaagc gtgattgtta cgctttttag
     4201 gaatgtttgt ctggtttgtc ttgggtttcg atgtcatcat atacctcgtc gacttacagc
     4261 cgggaacatc tcaaccttgc gttgaataaa tatacgagtt ttctggccgc ccagttttta
     4321 ccaattgtat ttacttggtg actctagctg agtatagcac cgttcgtaat ttcagaaacg
     4381 aggtagggct ctctacactg tatcaaggta aaacattgtc catcccaaaa atgtgagaat
     4441 ccatggacaa atcattatcg cagttctcga agctggtgct gctcggtgcg ggcgctttag
     4501 ccgttatcgt ggggcccatt ctttacttct tccccgaaga cacgggtact tattttgcct
     4561 ggtcgatcca gcacccactt acgccggttt atatgggtgc ctcttacctg gcggggattg
     4621 ggaatgtcct tgccgtaaga gccaacaaat ggtcattggc gcgtgtccag ttgccagcca
     4681 tcatcttttt cacgcttacc atgctggttg caacgttgat gcacatcgag attttcaatt
     4741 ggtcgcatcc ggttgcgtgg ggatggctag ctgtgtatgt gattagtccg gtcgcagccg
     4801 tgttggtgtt tttgcaaatg gaacgcggct ttcaaagtcc gcaattggtg tcgaacaaac
     4861 tgcccgcctc attctcaccg gtgatgctta ttttcgcagc aatttatgca gcaattggct
     4921 tggcgctttt ctttttcccc gaacagactg catccctatg ggcatggtca ctcaccccac
     4981 tgaccgcgcg cgttatcggg ggttggtgca tttccgccgc ggccttgcag tatatgctcg
     5041 cacaacagaa gacccttcac acggctcggg ttggaattgt tgcgaatttc ctgataacca
     5101 gcctgcatct tatcggagcc ttcctgcact tttcagaatt caacggctcg cagatttcga
     5161 tatggctcta tttattgctg aatctatctg tgaatggatt ttcgatttac agttgggtgc
     5221 aatccagagc tcatacaaaa taactttagg gcagatcgcc cagtctcctc tacttgttct
     5281 cctgttccca gcgactgatg gcattctgca ggcaggtcag tcgggtagcg ttgggcgttt
     5341 tcccacgctc atactcgatg agtcgctcaa ctgcagaacg atcaccaccc actttgcgga
     5401 gcagatcatc aaataaggct ttctctctca atacgccggt aaataactcc tcagcataat
     5461 caggttctcc gcggatcttc cgcactatag actcgatcat cagagcaagc ttgctcttcg
     5521 gacggaacct gggaggttcg tttaactctg tatcgaccgg gtcttccaga tacacaccgc
     5581 agttgggaca tttcaacagc ccatgataaa tatacgcgcc gcaattgccg cattcgactt
     5641 catcatcctg gggtggggga gtgaggctgg tgggcatgaa aagattgtac tctggttaaa
     5701 gacttcagag gtctatgact t
//
LOCUS       DASTJP010000287         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_226693, whole genome shotgun sequence.
ACCESSION   DASTJP010000287 DASTJP010000000
VERSION     DASTJP010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226693"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /gene="glnA"
                     /locus_tag="VFQ23_08650"
     CDS             complement(<1..1211)
                     /gene="glnA"
                     /locus_tag="VFQ23_08650"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET9906698.1"
                     /translation="MDAKDLLKRVKEDGVKFVSLQFNDVTGTVKSVDMPIHGLDGALE
                     DGVWFDGSSVEGFARIQESDMLLRLDPDTYAVLPWSSEERRRARVFCDIFTPDGKPFE
                     GDPRGALKRLMAKIAERGWTYNIGPEPEFFLFKGMDGHGVHPVPHDVGGYFDFSAFDE
                     AVRVRTQLMDALHSMGLEVEVGHHEVALGQHEIDFRFANAVRAADNVLTLKYTVKAIA
                     SQHGLTASFMPKPVFGINGSGMHCHQSLFDKKGNNLFFDKKDEYHLSQIAYSFIAGQL
                     KHARGLAALVAPTVNSYKRLVPGYEAPVYIGWAQRNRSALIRIPRYTEGRDKSMRAEL
                     RFPDPSSNPYLAFTGMLAAALDGIDNNLTPAKPLNNVNLYHLDAKERKKLGVGELPGS
                     LAEALSELEKDA"
     gene            complement(1302..2231)
                     /locus_tag="VFQ23_08655"
     CDS             complement(1302..2231)
                     /locus_tag="VFQ23_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9906699.1"
                     /translation="MKTKVWIALLALYIVWGSTYLAIRIAVETLPPFLHAGLRFLISG
                     LVLVVWRRAAGDAMPTRNQWKSLTIIGTLLLLGGNSLVAVAEQRIASGIAALIIGTVP
                     LWLVVIEFLRPGGTRPAWQALVGLIIGFAGIYLLVGPTELSGGLQFDALGTILLMIAA
                     FLWSLGSIYSRSADLPKSALMMTGGEMLAGSIPIFIVSLLFGEWGTFNIAQVSTASWL
                     ALLYLITFGSMVGFVAYIWLLQNAPISLVATYAYVNPLVAVLLGNWFAAEELTPRILI
                     GAVIIIGSVVFINSARKAKVRTEPRPAVADSDD"
BASE COUNT          579 a          595 c          575 g          514 t
ORIGIN      
        1 gcatccttct cgagttcgct caacgcctct gccaacgaac cgggtaattc gccgacacct
       61 aacttcttgc gttccttcgc gtcgagatgg tagagattaa cgttgttgag cggcttggcg
      121 ggagtgaggt tattgtcgat accatccagc gcggcggcga gcatccctgt aaatgccagg
      181 tatggattcg aggatgggtc tgggaagcgc aattctgcac gcatggattt atcacgacct
      241 tccgtgtagc gcgggatgcg gatcaatgca gaacggttac gctgtgccca accgatatag
      301 accggtgctt cataaccggg gacaagtctc ttataggagt ttactgtcgg cgcgaccagg
      361 gcggccagcc ctcgtgcgtg cttgagctgc ccggcaatga acgagtatgc aatctgtgaa
      421 aggtgatatt catctttttt atcgaaaaat aaattgttgc cttttttgtc aaacagactt
      481 tggtgacaat gcatgcctga tccgttgatg ccgaagacag gtttgggcat gaaggaggcg
      541 gtcagcccat gttgtgatgc aatggctttg acagtgtatt tgagagttaa tacattatct
      601 gccgcccgaa ccgcgtttgc aaagcggaag tctatttcat gctggcctag ggccacttca
      661 tgatgaccca cctccacttc gagtcccata gaatgcaaag cgtccatcag ctgggtacga
      721 acgcgtacag cttcatcgaa ggccgagaag tcaaagtatc cgcccacgtc atgtggaacg
      781 ggatgaacgc cgtgtccatc cattcccttg aagagaaaga attcaggctc aggcccgatg
      841 ttataggtcc agccgcgttc ggcgatcttt gccatcaatc gcttcaatgc tccacgcggg
      901 tcaccttcaa agggcttgcc gtccggcgtg aaaatatcgc agaagacgcg ggctcggcgg
      961 cgctcttcag aactccaggg cagaacggca taggtatccg ggtcgagacg cagaagcatg
     1021 tcgctctcct gaatgcgagc gaagccctcc accgaggagc catcgaacca gactccgtct
     1081 tcgagcgcgc catccagacc gtggataggc atatcaacgc tctttaccgt gccagtaaca
     1141 tcgttgaact gcagtgaaac aaacttaaca ccatcttcct ttacacgttt taacaggtct
     1201 ttcgcgtcca taatagattc tcctttgatt attggattat atcgttgcga ggagtggcgc
     1261 gagcgccacg acgaagcaat ctcatgcttt tggatatgat tctaatcatc gctatctgcg
     1321 acagctggcc gcggttctgt tctgactttg gccttccgcg cgctattgat aaaaaccaca
     1381 gacccgataa tgatcaccgc gccgataagg atgcggggcg tcagttcttc cgcggcaaac
     1441 caatttccca ataataccgc cactaacggg tttacgtatg cataggttgc tacaagtgaa
     1501 atgggcgcgt tttgcaatag ccaaatgtag gcaacaaacc caaccatgga accaaacgta
     1561 atgagatata acaaggcaag ccatgatgca gtagaaacct gcgcgatgtt aaacgtcccc
     1621 cactcgccaa ataggaggct gacgataaag atcggaatac taccggccag catttctccg
     1681 cctgtcatca tcaatgcgga tttcggcaaa tccgcactac gactatagat cgagcctagt
     1741 gaccacagaa atgcagcgat catcaggagg attgtaccaa gggcatcaaa ctgtaatcca
     1801 ccagataatt cggtaggacc aaccaacaaa tatataccag caaaacctat gatcagtcca
     1861 acgagtgcct gccacgcagg tcgagttccg ccaggtcgta agaattcaat cacgacgagc
     1921 cacaaaggga cggttccgat aatgagggcc gcgattcctg aggcgatcct ttgctctgcg
     1981 acagccacca gcgagttgcc cccgagcaaa agcaacgtgc caatgatcgt cagtgacttc
     2041 cactgattac gggttggcat ggcatccccg gccgcccttc gccagacgac gaggaccagg
     2101 ccggagataa gaaatcgaag tcccgcgtga aggaatggcg ggagagtctc gactgcaatc
     2161 cgaattgcaa gataggtcga accccaaacg atatacagcg caagcaacgc aatccagact
     2221 tttgttttca tttattcctt atgaatgtct tttggtttag tgg
//
LOCUS       DASTJP010000288         8022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1446571, whole genome shotgun sequence.
ACCESSION   DASTJP010000288 DASTJP010000000
VERSION     DASTJP010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446571"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFQ23_08660"
     CDS             <1..345
                     /locus_tag="VFQ23_08660"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET9906700.1"
                     /translation="AIFNPLGVGLAAGTGGAIGELSGYLAGFSGQAVIERTDVYAKIK
                     PWVDKYGGWAILVLSAIPNPLFDVAGIAAGIAKMPFWTFFFFVWIGQIIKMTIFAYAG
                     RYSIDLLSIFIK"
     gene            355..1713
                     /locus_tag="VFQ23_08665"
     CDS             355..1713
                     /locus_tag="VFQ23_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9906701.1"
                     /translation="MSDFTKIDSYLENNLDKSIAELSKLVAQPSISAQGIGLKECANL
                     VADMLRARGFSAEVMDTEGAPVVFGERKGKSDKTLLFYNHYDVQPPEPLELWDSPPFE
                     PSLRDGKLYGRGVSDDKSHIVARLHAIDALLDVDGELPCNIKFIIEGEEETASVHLHE
                     FVRDNKEKLAADACIWEFGSVDHRGVPMQYLGLRGICYVELSVESLGTDVHSGIGGSI
                     FPNAAWRLVWALSTLKGQDERIRIPGFYDDVKEPSVRDRELMEALPDVAEEYRRRYGV
                     KEFLKGLTGGTDLKMEEVFVPTCTICGLTSGYQGPGSKTVLPAKASAKVDFRLVPDQK
                     PEDIFRKLRAHLDAEGFQDVQITFLGGEPAARTDPDDSFVQTVVKTSEEIYEFPMELV
                     PMIGGSGPSYPFVHDLGLPVATAGLGYPDTRAHAPNENIRLDLYLQHARHMARLLKEF
                     SK"
     gene            complement(1763..2518)
                     /locus_tag="VFQ23_08670"
     CDS             complement(1763..2518)
                     /locus_tag="VFQ23_08670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9906702.1"
                     /translation="MPERLQKILAQAGYGSRRACEDFISAGRVRVNGQIAILGQKADP
                     AKDKITVDGKPIAAPESPTYVALYKPRNVLSTVESERGDDRQTVRDLVDAPGHLYPVG
                     RLDFESEGLVLMTNDGELTNQLTHPRYGHQKEYKVLLAKRPDHDQLEAWKRGVVLEDG
                     YKTAPSDVRFETAQGKGAWVRVIMGEGRKRQIREICRQLGLPIVRILRIRIGALKLGN
                     LKPRQWRYLTMQEVDELKGKAVNKNTRRDPLRR"
     gene            3228..3905
                     /locus_tag="VFQ23_08675"
     CDS             3228..3905
                     /locus_tag="VFQ23_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906703.1"
                     /translation="MSASVRVAILDDHQSVIDGYIFRLSQTSKIQVVATARFGQDLDS
                     LLAKSSVDVLILDVQVATSIDNPSPYPILHVIPNLLKSYDGLAVLVISMYNQPSLIKA
                     VMDAGASGYILKDDEPTIKELGSVISTIAEGGIHLSQLAYQQLFRKLPKESILTVRQL
                     QILSICAAYPDATTGEIADKLGVAHSTVRNLLSGAYLSLHVRSRASAVDKARHLGLIT
                     PPATMEY"
     gene            complement(3918..4874)
                     /locus_tag="VFQ23_08680"
     CDS             complement(3918..4874)
                     /locus_tag="VFQ23_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9906704.1"
                     /translation="MDIYQSIVDYVSTVPLTERWPKIPTLFARAASRRPRHWRLPLLS
                     CEAVGGSLEQAVPAVTAIGCLHISILLVDDLLDADIRGEHHRLGQPATANLAIAFQAA
                     GLAAVAQSNVAPLSKLAMMVSLNKMMLTTAIGQYLDVQNTYDEETYWQLVRLKSAPFF
                     GAALQIGAILGSGTNQYMAEQIKQVGQLYGEMIQVHDDLNDTLTVPANSDWTLGRMPL
                     PILFASLVNHPDRDRFIHLRNSITDPDALAEAQTILIRSGAVSYCLDQLLSKSKVARE
                     ILAGTSLVHHEKLDNLLREIVSPVQRLLSIKGIDYHGSQLEI"
     gene            complement(4929..5090)
                     /locus_tag="VFQ23_08685"
     CDS             complement(4929..5090)
                     /locus_tag="VFQ23_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906705.1"
                     /translation="MLTMQAFYSQLQTSGKEPDGYFTLLDRLTQCTLSEVMAVRSEMI
                     WELWDTPSA"
     gene            5167..7500
                     /locus_tag="VFQ23_08690"
     CDS             5167..7500
                     /locus_tag="VFQ23_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9906706.1"
                     /translation="MNYNCITSFQGKFFNSGRDMLDKDQLLKTYAAYFLAIATLIILL
                     AYTVAKFAVVPYLGFIFNPSDGSLIRIFTSTGSRPSLQTNDKLLRVGTVEWDEFKANS
                     RKTLINKQATGEIIEILILRDGVEKSISWHYPGPNRAEIVDRLNSVLILAYAFWLSGL
                     VTILFIRPRDSRWILMIIFLFLTAIWVACGSISAWHIWESAILLRMAIWMCVPVYMHF
                     HWFFPRELRRVPTGLWWCLYISAAVLAGLEWFERLPRNAYYIGFFIALFSTFGLLIFR
                     YIALPKQRQDVRLIVIGSMVAMLPALALSLAGILDKYPWFGGLALIGLPLLPGAYFYN
                     VYYRQIGGLNVRTNRLISLYVYFALIGVPSALIISFIISQIETLEAATFVSVLSSILI
                     ALVSIAIFPFFQRTFENRVLRIPHPPTSIISTYSDRITTSLNRSSLKGLLEVEILPSL
                     LIRQSALIQLDEHHQSAVLYSNEVSAIQPLSDVDVSDLVETAGRYMPSSVGTRNEIFR
                     WIRIVLPLKVENKLVGLWLLGSRDPDDLYYQAEISVLETIANHTAIALVNIAQAERLE
                     MLHKANIERQEIERTSLARDLHDEILGQLANLFNSVAKQAGPEFHEHYQKLTARIRQI
                     GSELRPAMLTYGLRSAIEELVDSLSDRVGDEVIISMELPASEIRYPAAIEEHLYRMVQ
                     QACENSLRHAQAKLVRIHGSLKPEAVALVIEDDGIGFERLTDLTLEGLLASKHYGLVG
                     MYERAAIIGSELRIDPKPGFGTQIHIKWHAFKMGYIA"
     gene            7639..>8022
                     /locus_tag="VFQ23_08695"
     CDS             7639..>8022
                     /locus_tag="VFQ23_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906707.1"
                     /translation="MTDFGDVLRKCRQSCREHQNPRRPLSQGRLGELLGKELNMQGGG
                     YSAAAISDWELGKSKISADQRLVLMSLIKILHELGGLRTSAEANNLLEAGYYRALNPD
                     EKRRIFPQELHTTASEDPTYKSGSQQ"
BASE COUNT         2067 a         1837 c         1903 g         2215 t
ORIGIN      
        1 gcgatcttca atccgcttgg ggtcggtctt gcagcaggga cgggcggcgc gatcggtgag
       61 ctttccggat atctcgccgg ctttagcggc caagccgtga ttgagcgaac tgatgtttat
      121 gcaaaaatta aaccctgggt agataaatac ggaggttggg caatattagt tctgtctgcc
      181 atcccgaatc cgctttttga tgtagcggga attgctgcag gcattgcgaa aatgccgttt
      241 tggactttct ttttctttgt ctggatcggt caaattatca agatgaccat ttttgcctac
      301 gcgggtagat attcaattga tttattatcg atattcatca aataggagaa tgttatgtct
      361 gacttcacca aaattgattc atatctcgaa aacaatctgg ataagagcat cgctgaacta
      421 tccaaactcg ttgcccagcc gagtatcagt gcgcagggga ttggtctgaa ggagtgtgcg
      481 aatcttgttg cagatatgct tcgcgcgcgt gggttcagtg cagaggtgat ggacaccgaa
      541 ggtgcacccg tggtctttgg cgagcgaaaa ggaaaaagcg acaagacgct actcttctac
      601 aatcattatg atgttcagcc ccccgaaccg cttgaattat gggacagccc accctttgaa
      661 cccagcctgc gcgatggcaa actatacggg cgcggggtca gtgacgataa atctcacatc
      721 gttgcacgct tgcatgccat cgatgcgctc ctggacgtag atggagaatt accttgtaat
      781 atcaaattca tcatcgaggg cgaagaggaa acagccagtg tgcatctgca tgagtttgtg
      841 cgcgataaca aagaaaagct cgcggcagat gcctgtattt gggaatttgg cagtgtagac
      901 catcgcggcg tcccgatgca gtatctcggc ttgcgtggga tctgctatgt ggaactttcc
      961 gtcgaatcgc ttgggacaga cgtccactcg gggatcggag gttcgatctt cccaaatgcg
     1021 gcgtggcggt tggtgtgggc actatccacg cttaaagggc aggatgagcg gattcgcatc
     1081 cctggctttt acgacgatgt gaaggaaccc tccgtacgcg accgcgaatt aatggaagct
     1141 ctcccggatg tcgcagagga atacagaagg cgctatgggg taaaagaatt tctcaaagga
     1201 ctcactggcg gcacagacct caaaatggag gaagtgttcg ttcccacctg taccatttgt
     1261 ggactcacca gcggatatca aggtccgggt tcgaagaccg ttttgccagc gaaggcatct
     1321 gccaaggtgg acttccgcct cgtcccggat cagaagccgg aagatatctt cagaaaactc
     1381 cgtgcccacc tggacgcaga aggtttccag gatgtgcaaa ttacatttct aggtggcgag
     1441 cccgccgctc gtaccgaccc ggatgacagt ttcgttcaaa ctgttgtaaa aacatcagaa
     1501 gagatctatg agtttccgat ggaattggtc ccaatgatcg gtgggtcagg tccgagttat
     1561 ccatttgtgc atgatctcgg gttgccggtt gcgaccgcag gcctgggtta cccggatacc
     1621 cgcgcacacg cccccaatga aaacatccgt cttgatttat atttgcagca cgcgcgtcac
     1681 atggcgcgtt tgctcaaaga attttcgaaa taactaaatc cttgcctgcc ccaaatctat
     1741 tcgatttggg gcaggtgacg ccttaacggc ggaggggatc acgtctggta tttttattaa
     1801 cagccttccc cttcaactcg tcaacttcct gcatggttag atatcgccat tggcgcggtt
     1861 tgagattgcc caatttaagc gcgccgatcc gaatacgcaa aatcctcacg ataggtaatc
     1921 ccagctgcct acaaatctca cggatttgtc gctttcgtcc ttcgcccatg atgacacgca
     1981 cccaggcacc tttcccctgc gcggtttcaa atcgaacatc ggaaggcgcg gtcttatagc
     2041 cgtcttccaa aacgacaccg cgtttccagg cttccaactg atcatgatcc gggcgcttcg
     2101 ccagtaagac tttgtattct ttttgatgtc cgtatcttgg atgagtaagt tgattcgtta
     2161 actcgccatc attagtcatc aatactagac cttcgctttc aaaatccaat ctccccaccg
     2221 gatatagatg acccggcgca tctacaaggt cgcgcacggt ttggcgatca tctccacgtt
     2281 cgctttccac cgtagataat acattgcgtg gtttgtacag agccacatag gtcggtgatt
     2341 caggcgccgc aatcggtttt ccatcgacgg tgatcttatc ttttgccgga tcagctttct
     2401 gccccaagat agcgatttgt ccattgacgc gcacgcgccc ggcagagata aaatcctcac
     2461 aagcgcggcg ggatccgtat cccgcttgag ctaatatttt ttgaagtcgt tcaggcatgg
     2521 tagcaaatta taaccgttta catactgggc aagtcgttgg aattttcagg gagcgaataa
     2581 ttttgtgggt attttcaaaa tttgccgttg caaattgcgc acaagcccat gatgtgcgcc
     2641 actttccctg gcttgaaagt tacgaaacgt atcccaaaat agtagatttt ggtccaatcc
     2701 tgcgccggaa tcagtccgac ggatctcaaa aatgaaaatg tgcaattaat tgggaaactt
     2761 ttgcttctga aagtcgaacg ccgagggatg gcaagtgagt tccaagagtg accgaccgtg
     2821 cgatcctgga aatatgtatt agagggttgt cgctttcaat gttttctcat aaattatgac
     2881 gaatctggtt ctgcctgatc ctacaagtaa tctcaaaaac atcatcctga gaggagcgac
     2941 acttgcaccg cactgccgtt cgttgcagca caggtgagga tctccaacac cgaaagtacg
     3001 agactctgcg gtcgccaaca gcgctccctc acgccgtccc gaagtggtgt cccttcggga
     3061 gagtgacacg gggttttttg agacaagcta taaataacat tgccggttca gtaaatggac
     3121 agagcatggc acagcataga caaatgtact ttttcatagg acaatacttg aaattttaca
     3181 aatacctgct atcctttaga tatctcctta tatttgagac ttaatacatg tccgcttcag
     3241 tacgtgtagc aattttggat gaccatcaga gtgtaattga tggctatata ttccgtctaa
     3301 gccaaacgtc caagattcaa gtagtggcta cagcccgatt tgggcaagac ttagattctc
     3361 ttctagccaa gagttcggtt gatgttttga ttttggacgt acaggtggct acgtctatag
     3421 ataatcccag cccttaccct attttgcatg ttattccaaa tcttcttaag tcgtatgatg
     3481 ggcttgctgt tcttgtgatc tcaatgtata accaaccttc actgatcaag gccgtgatgg
     3541 atgctggggc gagcggctat attctcaaag atgacgaacc aacaattaag gagctaggca
     3601 gtgttatttc tacaatagca gaaggtggaa tccatctaag tcagctagct tatcaacagt
     3661 tatttagaaa gctaccgaaa gagtctatac tgacggttcg acagttacaa attctatcaa
     3721 tatgtgcggc atatccagat gctaccactg gggaaatcgc tgataagctc ggagtcgctc
     3781 attcgactgt gcggaatttg ctttcgggtg cttatctcag cctccacgta cgaagtcggg
     3841 catcggccgt agataaggcg cgccatttag gactaattac tccgcccgcc acgatggaat
     3901 attgatacgt tgaaaagcta gatttctagt tgagagccat gataatcaat gcctttaatg
     3961 gagaggagtc tctgaacagg actcacaatc tcacgtagca aattgtcgag tttttcatgg
     4021 tgaactagtg acgtaccggc caaaatctcc ctcgcaactt tggatttaga gagaagttga
     4081 tctagacaat aactaacggc accacttcgg ataagaatag tttgtgcttc agcaagtgca
     4141 tctggatcgg ttattgaatt cctgaggtga ataaatcgat ctcgatctgg atgattcacc
     4201 agagaagcaa aaaggatcgg aaggggcatg cgcccgagcg tccagtctga atttgcgggg
     4261 accgttaagg tgtcattcag gtcgtcatgt acctggatca tttcgccata aagttgccca
     4321 acctgcttga tttgctcagc catgtattga ttggtccccg atcccaatat tgcacctatc
     4381 tgcagcgcag cgccgaaaaa tggtgcgctt ttcagacgta caagttgcca gtaagtttcc
     4441 tcatcataag tattttgaac atccaaatat tgccctatgg cagtagttaa catcatctta
     4501 ttaagactta ccatcattgc taacttggat aacggtgcaa cattactttg cgcaacagcc
     4561 gctagtccgg ctgcctggaa cgcaattgct aaatttgcgg tggctggctg accaagacga
     4621 tgatgttcac cccgtatatc ggcgtctaat aagtcatcca ctaagagtat gctaatgtgc
     4681 aagcaaccaa tagctgtcac agctggcact gcctgttcaa ggctacctcc tacagcttcg
     4741 caagacagca gaggcagccg ccagtgacga ggccttctgg atgcagcgcg cgcaaataaa
     4801 gtgggtatct tcggccatcg ctcggtgagt ggcaccgtcg aaacataatc gacaattgat
     4861 tgataaatat ccatactacg ctcctcgatg gctggctggg atatacccag ccagcattat
     4921 ttgccgaact acgcacttgg cgtgtcccaa agttcccaga tcatctcact tctcacagcc
     4981 atgacctcac tcaaagtgca ttgggtcagc ctatcgagca acgtgaagta tccatcaggt
     5041 tctttgcctg aggtttgtaa ctgagaatag aaagcttgca tggtcaacat ttttagaatc
     5101 tcctttcatc ttaattttgg atagttgtgc ttttctgata gtggttaaag tttggactct
     5161 ttttcaatga actataattg catcacctcc tttcaaggaa aatttttcaa ttcaggtaga
     5221 gacatgttgg ataaggatca acttctaaaa acctatgcgg cttacttcct tgcaatagca
     5281 actctgatca tcctattagc atatacagtt gccaaatttg cagttgtccc ttatctaggc
     5341 ttcatcttta atccttcgga tggaagcctt attcgaatat tcacttcgac cggatcccgg
     5401 cctagtctcc aaacaaatga taaattactc agggtaggca cagtggagtg ggatgaattc
     5461 aaagcgaata gccgtaaaac cttgatcaat aagcaagcaa ctggagaaat catcgaaatc
     5521 ctcatcctca gggatggggt agagaaatcc atttcttggc actatccggg accaaatcga
     5581 gccgaaattg ttgataggct caatagtgtc ttaattttgg cttatgcttt ttggctttca
     5641 gggctcgtga ccattctgtt cattcgcccc cgcgattccc gctggatctt gatgataatt
     5701 tttctttttt tgacggcgat ctgggttgct tgtggtagca tttctgcttg gcacatttgg
     5761 gaaagtgcaa tattgttgcg gatggcaatt tggatgtgtg tgcccgttta tatgcatttt
     5821 cactggttct ttcccaggga actgcgccgc gtacctaccg gcctgtggtg gtgcctgtat
     5881 ataagtgcgg ccgttttagc aggtttagag tggtttgaga ggctaccaag gaacgcgtac
     5941 tacattggtt tcttcattgc cttatttagc accttcgggc ttctcatttt tcgctatata
     6001 gccctgccta agcaacgcca agatgtgaga ttgatcgtca tcggatcaat ggttgcgatg
     6061 ttaccagcct tagcgttaag ccttgctgga attcttgaca aatatccttg gtttggcggc
     6121 ctggcactga ttggcctgcc cctcctgcct ggggcatact tttataatgt atattaccga
     6181 caaattggtg gtcttaatgt acggacgaat cgcctaattt ctttatacgt ttattttgct
     6241 ttgatcgggg taccgtccgc cctaatcatt tcgttcatta tcagtcagat tgaaacgctt
     6301 gaggctgcta cgtttgtcag tgtactctca tccatcctga ttgctctagt ttcaattgct
     6361 attttcccat tcttccagcg tactttcgaa aatcgcgtgt tgcgaattcc gcatcctcca
     6421 acgagcatta tttcaactta ttcagaccga attactacaa gtcttaatcg gagcagtctg
     6481 aaaggtttat tggaagtcga aattcttccc agtctcctca ttcgtcaatc tgccttgata
     6541 cagttagatg agcatcacca atcggctgtg ctttattcaa atgaggtaag tgccattcaa
     6601 ccgctttccg atgttgatgt ttcagactta gttgaaacgg ctggaagata catgccatct
     6661 tccgtaggca ccaggaatga gatatttcgc tggatacgaa tagttcttcc tcttaaggtg
     6721 gaaaataagc tagtcggctt gtggttgctt ggaagtcgcg atcctgatga cttgtactat
     6781 caggcagaga tctcagtttt agaaacgatt gcaaatcata cagcgatcgc attagtaaat
     6841 attgctcagg cagagcgact ggaaatgtta cacaaagcca atattgaacg tcaggaaatc
     6901 gaacggacca gcctggctag ggatcttcat gatgaaatac tgggtcagct ggcaaaccta
     6961 ttcaacagcg ttgcgaagca agctggccct gaatttcatg aacattacca gaaattaact
     7021 gcacgcatcc gacagatagg gagtgaacta agaccggcaa tgttgacgta tggattaagg
     7081 tcagcaatcg aagagctggt tgatagcctc tctgacagag tcggtgacga agttattatc
     7141 tcgatggagt tacctgcatc tgaaatacgc tatccggctg ccatagaaga acacctttac
     7201 cgcatggtac aacaagcatg tgagaactct cttcgacacg ctcaagccaa gcttgtccga
     7261 attcatggat ctttaaagcc ggaagcagtg gccttggtta tcgaggatga cggtattggc
     7321 tttgaaaggc taacagattt gacattggag ggtttattag catcgaagca ctatggattg
     7381 gtcggcatgt atgaaagggc cgcaatcatt ggctcagaat tgcgtatcga tccaaagccc
     7441 ggcttcggaa ctcaaataca tatcaaatgg catgcattca aaatgggcta catagcttaa
     7501 tattgcataa tggtaattaa gttgttaagg ttcgaaggac aaattgcttt ttgcaatatc
     7561 gttatacaat gatgctgtac aataatccat ctgcattcac ttgaatcttt caagtgttgc
     7621 ttgaagtcag gtgttaacat gactgatttt ggcgatgtcc ttcgaaaatg ccgtcaaagt
     7681 tgtagagagc atcaaaaccc gagacgccca ctctcccaag gccggctggg tgaattgttg
     7741 ggcaaagagt tgaatatgca agggggagga tacagtgccg cagcaattag tgactgggaa
     7801 cttggcaaaa gtaaaatcag tgctgatcag agattggtat tgatgagcct gattaagatt
     7861 ttgcatgaac taggtggttt aaggacctca gctgaagcta ataatctttt ggaagctgga
     7921 tattatcgcg cgctaaatcc tgatgaaaag cggcgaattt tcccccaaga gttgcacact
     7981 acagcatcag aagatccaac ttataagtcc ggtagtcagc ag
//
LOCUS       DASTJP010000289        12536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_864771, whole genome shotgun sequence.
ACCESSION   DASTJP010000289 DASTJP010000000
VERSION     DASTJP010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864771"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VFQ23_08700"
     CDS             <1..1354
                     /locus_tag="VFQ23_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906708.1"
                     /translation="FADVYTDQHIYLYTNAYAETNDHSYSIGYTASNSYANTDVHAIS
                     DADVDFHAVNYTNRFCYAFADIDVYTHSNANACANEYIYSNRHPASNTNLYANVHPIA
                     DGNSHTFANRFGYAFADLYTHLHLYSNTQHYSFKHCYSDVYSFQHANSNFNADFYTNI
                     YTDQYPYTNADAYTQTHVDTNSDVYPASDKNSNLNSDSIVNTNSNIHAHCISDSASDE
                     YIYTKCNAASHTYPNVYLHCVRNPHSDIHTFEYAFADINLHFHKYVYTDFDSHTKTNA
                     HVYANLYPTSIADFNVYSNSLVDTNKYVYTYIHSNPATYCHAHADVDSASNANLHANS
                     DTNSVAHSLLHADSDIYSDEYIYLDPNLYTHAYTKQHRDKYANLIAYSYSDSFEHTDE
                     DIYKYTNIHTDLDALPNADLNTVAHANTDANPNCDPHTIAYSNTNSICDAYTDSKQRL
                     "
     gene            1320..2234
                     /locus_tag="VFQ23_08705"
     CDS             1320..2234
                     /locus_tag="VFQ23_08705"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9906709.1"
                     /translation="MTRTPTANRGCDRIEFVADLTIPDNSVFSPGSAFTKIWRLRNSG
                     TCTWTTSYRVVLVSGDAMGGQNGMPLPQDVAPGQTIDLAMNFTAPLVQGSYRGNWQIR
                     NERGEIFGTTSTANRPFWVTITVRAPLLTGTSYDFVANACSAQWYSGNGNLNCPGTNN
                     DRNGFILRQSNARLEDNTLVNRPSLLTAPQNVFNGYVRGVYPSYRVQNGDHFQAIVNC
                     EGQATSCGVLFRLDYQLSDGLIRDFWAFGERYEGSYFTVDLDLSPLAGQDVRFVLSVL
                     SLGSASGDRALWVEPRIVRSGVLITPTP"
     gene            2299..3222
                     /locus_tag="VFQ23_08710"
     CDS             2299..3222
                     /locus_tag="VFQ23_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906710.1"
                     /translation="MDIRAGIIAAVALSVIGALWIFRSGIRSLQAARRLTFYRIRRQR
                     ERSGWITLLVALSLLSCGIWLSFFGEPVAYRYFPPSPTITRTPTITLSPTITLSPTIT
                     LSPTITDTPSITDTSTITPTPFIPPVIEAMFESVVTPNPNSVFSPLQFTRNCDSFTEF
                     EAATVFDNPITYMCAVFTYDQMTPGAQWTALWVRDGELVHYETIPWNGETGGFGFTEW
                     EAPAEEWLPGTYEVQVFVGLDWKVVGQFIVQGDAPTPRPTQTPSPTLTPSRTATVTRT
                     PTATRTPTATRTFTPTRTPTPATPTSSTVTP"
     gene            3294..3782
                     /locus_tag="VFQ23_08715"
     CDS             3294..3782
                     /locus_tag="VFQ23_08715"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9906711.1"
                     /translation="MSQKSFQDHYPDVFAHCYGCGKLNEHGHQIKSYWDGEESVCHFL
                     PKPYYMAIPGYVYGGLLASLIDCHGTGTAAAAMYKSLKESDPNTEPNTRFLTASLHVD
                     YLKPTPLGIELEIRGRVKELKGRKVVIEEWLLANGVVTVRGEVIAVQVPESMVEELVK
                     GK"
     gene            3863..4894
                     /locus_tag="VFQ23_08720"
     CDS             3863..4894
                     /locus_tag="VFQ23_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9906712.1"
                     /translation="MSKSVYIGTDGGATTSKVGGVWDDGSTISTKLLQASTNSTQGPE
                     SVVRSWVDAIDDYLSKNSLTWEQVRGVGLAVPGPFQRYGVFDHSANLPDSFTGFDVHT
                     AYRNALSERAGRAIPLTVGNDGDMGGVAEAQHARGNTTGTVLMLAPGSGLGCAYIDEN
                     GIALDGDTLAGMEAGHMPALLHLLNVKAYPCGCGRTWGCIEVYTCLAGLPHLLAEKLE
                     QYPEHDLAKSSLSMKERALSLRGLAQKGDQLATELFDFQAKALGLHVANLAMALDPKY
                     VVIGGGLMDPEATTIDFRERYLNIVRSTAREYLWPYQRDVISIGPAALGDLSQAIGAA
                     LSALYRDRA"
     gene            complement(4959..5867)
                     /locus_tag="VFQ23_08725"
     CDS             complement(4959..5867)
                     /locus_tag="VFQ23_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9906713.1"
                     /translation="MPQDVFANTSANWWEGGQKVKPGDKFQPRKLNTPLDKLARKHAG
                     RRSLTKTERKHGRYIKARPAGEKKDDIAFDATFRAAAPFQKQREEKRKRLAFAIESSD
                     LQRKIRVKRVANLVLFLVDASWSMAVAERMNATKGAILSLLTDAYQRRDRVGLIVFQK
                     DRATLVLPPTNSVQLAQRALMDIPVGGKTPLSAGLFLAADVLRQEKVHHPDVEPLLIV
                     LTDGAGNVSIGALPPQEESYKFAELIAHEKIKCVVINMEHAAFDQGLAQQLANHLKAP
                     CYALSELKAENLYHAVKDEMAKPMKK"
     gene            complement(5918..7018)
                     /locus_tag="VFQ23_08730"
     CDS             complement(5918..7018)
                     /locus_tag="VFQ23_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9906714.1"
                     /translation="MPSIFPFTAIVGQERMKRALILNAVDTRIGGVLIRGERGTAKST
                     AARALAALLPKVKVVDECRFGCDPDKPATWCTECKERANGNNNTLPFHVKTTSFVNLP
                     VSATEDRVVGTLDIEQAIQKGERHFEPGVLASANRGLLYIDEVNLLDDHVVDVLLDSA
                     AMGVNMVEREGISFSHPARFILVGTMNPEEGELRPQLLDRFALSVDIVGIRSARERVL
                     IMERNLGFEQDADTFRNTFMPKEEELSHQIERGRELVGQVTHTSRDLLSIAALTASLN
                     VDGHRADLVILKAARAQAAFEGRTKINDHDIALAAELALPHRIKRTPFQQAEMTTEQL
                     QERIEQLAGQSVPSEAEQESESQQGQSEEKKT"
     gene            7234..8529
                     /locus_tag="VFQ23_08735"
     CDS             7234..8529
                     /locus_tag="VFQ23_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET9906715.1"
                     /translation="MTDQNEALPAEGNDLNQQGEQHTNNNNQTMESLLKEQDLNVDLP
                     QAGEIRTGVIASVSPTQILVSIGAKSEGVVSGRELDQLSTEEREALQVGQEVHVFVIN
                     PEDANGNVVLSLKRAQEQISWENVEKLLTSDNVIDSTIIGFNKGGLIVGVEGLRGFVP
                     SSQISAMRRSQSTGETPEQRWQKMIGQPITVRVIEVDRERRRLILSERAASTESRQSI
                     KERVIDELEEGKVYTGRVTSLANFGAFININGADGLVHLSELSWDHIDHPREVLEVGQ
                     EIKVKVINVDREKKRIGLSVRALQSDPWKSRVEKYSVGQLVEGVITRLTKFGAFARLE
                     GDIEGLIHISEIAEHRIEHPKEVLKEGEVKSLRVIRIDPEQHRIGLSLRKVDSAAFAD
                     KDFKLLTAEFETEDEPEAFEAPEASDDSPGESTEENTEA"
     gene            8572..9666
                     /locus_tag="VFQ23_08740"
     CDS             8572..9666
                     /locus_tag="VFQ23_08740"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9906716.1"
                     /translation="MPLIIDSHQDLAWNMLTYGRDYTRSVVETRRLETETLIPDQNGD
                     STIGWPEYQRGQVAVVFSTLFVTPARKKQPADTIWYTDANSAHRLYREQIDIYRQLAD
                     VHPDKFRLISSTKELDSVIEHWNKPVQEDEGHPVGLIYLMEGAEGIRSPHELGEWWDL
                     GLRMIGLAWAGTRFCGGTGEPGPLTAEGRQLLAAMADHKFILDLSHMDESSAFESLDR
                     YEGPVMASHANCAALMQGAETNRHLPDRVIRSLIEREGIIGSIPFNTFLKVGWKRDRG
                     SRREEVPLDVLIAHLDHICQLAGNSLHAGIGSDFDGGFGLQSIPPELDSIADLQLIAS
                     KLIARGYSELDAENILGGNWLRFLRKHLPS"
     gene            9663..10130
                     /locus_tag="VFQ23_08745"
     CDS             9663..10130
                     /locus_tag="VFQ23_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906717.1"
                     /translation="MSEEPILRISNLRIRFSLANVFLGFVIFAIGAKPNWFGWDRSPV
                     VGFIQIAVFLVGLALISAGGYVGLLALWGNEQRSILADIGSRLVGTGYVIAIFAGMAD
                     VFGLGTQPLPEVPYFGPWQAAGVFIGELVIIAGFLMIIPYRFEGKKRPESTNP"
     gene            complement(10233..>12536)
                     /locus_tag="VFQ23_08750"
     CDS             complement(10233..>12536)
                     /locus_tag="VFQ23_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9906718.1"
                     /translation="PGPVTITDDKVAGVACPNVNTVGNLNNNLDFNESISCTGTYAIT
                     QNDLNAGQVTNNATASVGGVTSALASATVRMAENKVLTIAKSASPTSYSAAGQTITYT
                     YTINNTGATTLGPAQFVVRDDHITTPINCGVATTTLATNQTVSCTAIYTITQNDMAVA
                     QITNSATASGAGASSINPATVTITNGVPPPGTPSNLPRGSTIRHKVAKGEWMLQIARC
                     YGANFEAVRLANPGVTDADLIFPFPKTPELVVPNIGSDGPIYGPVDCVVFYTVKAGDT
                     WESIARQFNADLEVLRAANVGKTVSNGVELKIPRNSARSTVPVTPQPDATRITFPAGT
                     NTVTLPGTVTAAGKVRYVLNATAGQTLSIEVTGAPTNELALAIYQPNGSALKAQDTTL
                     TWTGTIPTTGDYIIELVSVLVASNKPYNLKVTLSTPPPPTTPPPATGAIRVADINPGA
                     GNSDPAHLAVFNGTLYFSAVGNDNAGSELWKFEGPLSAASRVADINAGATGSDPSYLT
                     EFNGALYFKANGNDGAGVELWRYNGSATGRLTDINPGAGDSNPSHLAVFNNALYFSAN
                     GNDGKGVELWKTDGTTSSLAADIHNGSGDSSPAYLTVFKDALYFSATSNDGAGTELWK
                     FDGTTATRVSDINAGVGNSNPAYLEGFGGMLYFSANANDGTGTELWRYDGTNPPTRAA
                     DINTGAGDSAPTFLTDFNGALYFGAAGSDNAGIELWKFDGTTATRAADINPGGNSSPA
                     YLAVYNNELYFRADGGDSAGVELWKFR"
BASE COUNT         2984 a         3327 c         3142 g         3083 t
ORIGIN      
        1 cttcgcagac gtttacaccg accagcacat ttacctttac accaacgcgt acgccgagac
       61 caacgatcac agctactcca tcggctacac tgcgtccaac tcttacgcca acaccgacgt
      121 acacgccatc tctgacgccg acgttgactt tcacgccgtc aactacacca accgcttctg
      181 ctacgccttc gcagacattg acgtttacac ccactccaac gcgaacgcct gtgccaacga
      241 atacatctac tcaaaccgcc accccgcgtc caacacaaac ctttacgcca acgttcaccc
      301 catcgctgac ggcaacagtc acaccttcgc caaccgcttc ggctacgcct tcgcagacct
      361 ttacacccac ctacacctct actcaaacac ccagcattac tccttcaaac actgctacag
      421 cgacgtttac tccttccaac acgccaactc taactttaac gccgactttt acactaacat
      481 ctacaccgac caatacccat acaccaacgc cgacgcgtac acccagaccc acgttgacac
      541 taactccgac gtttaccccg cgtccgacaa gaactccaac ctcaactcgg actccatcgt
      601 taacaccaac tcaaacattc acgcccactg catctcggac tccgcttccg acgaatacat
      661 ttacaccaag tgcaacgccg cgtcccacac gtaccctaac gtctaccttc actgcgtccg
      721 caacccccac tctgacattc acaccttcga atacgccttc gcagacatta accttcactt
      781 ccacaaatac gtttacaccg acttcgactc gcacaccaag accaacgctc acgtctacgc
      841 caacctttac cccacgtcca tcgcggactt caacgtctac tccaacagcc tcgttgacac
      901 caacaaatac gtttacacct acatccactc aaaccccgcg acctactgtc acgcgcacgc
      961 cgacgttgac tccgcgtcca acgcgaacct tcacgctaac agcgacacca attccgtcgc
     1021 gcactccctc cttcacgccg acagtgacat ttacagcgac gaatacattt accttgaccc
     1081 caacctctac acccacgctt acaccaagca acaccgtgac aagtacgcaa acctcatcgc
     1141 ctactcctac tctgactcct tcgagcacac cgacgaggac atctacaagt acacaaacat
     1201 tcacaccgac cttgacgccc tcccgaacgc cgaccttaac accgtcgcgc acgccaacac
     1261 tgacgccaac ccgaactgcg acccgcacac catcgcgtac tccaacacga actcaatttg
     1321 tgacgcgtac accgacagca aacagaggct gtgaccggat cgagtttgtt gcagatctta
     1381 cgattcccga taactctgtc ttttcgccgg gcagtgcgtt tacaaaaatc tggcgattgc
     1441 gaaactctgg tacctgtacc tggacgacaa gctatcgtgt tgtgctggtc agtggtgacg
     1501 cgatgggtgg gcagaatggg atgcctctgc cacaggatgt ggcgcccggt cagacgatcg
     1561 atctcgcgat gaatttcaca gcgccactgg ttcagggaag ctatcgcgga aactggcaaa
     1621 tacggaatga gcgtggtgag atttttggta caacctccac cgccaatcgt ccgttctggg
     1681 taacaattac ggtcagagca ccgttattaa caggaaccag ctatgacttt gttgccaatg
     1741 cgtgttccgc gcagtggtat agcggaaacg gaaatcttaa ttgcccgggt acaaataatg
     1801 acagaaatgg atttatcttg cgccaaagca acgccagatt agaagacaac acccttgtaa
     1861 accgacccag tctgttgacg gctccccaaa atgtatttaa tgggtacgtc cgtggtgttt
     1921 atccttcata tcgggttcaa aatggcgacc acttccaggc catcgtgaac tgtgaaggcc
     1981 aggcgacttc ctgcggtgtg ctgttccgct tggattatca actttccgac ggactgatac
     2041 gtgacttctg ggcctttggc gagcgatatg aaggtagtta cttcacagtt gacctggact
     2101 tgagtccgct tgctggtcag gatgtacgat tcgtactttc ggttctctca ctcgggtctg
     2161 cttctggcga ccgcgcctta tgggttgagc cgcgtatcgt gagaagtgga gtattaatca
     2221 cgccaacacc ttaatttgcg taatggtctc ctccaaaagc tcataaaatt ctacatgcta
     2281 taatctttta gagattctat ggatattcgt gcaggcatta ttgccgctgt tgcattatca
     2341 gtcattggcg cattatggat atttcgttct gggatacgtt cgctgcaggc ggcgcggcgg
     2401 cttacgtttt accgaatcag gcgtcagcgg gagcggagtg gatggatcac gctacttgtc
     2461 gcgttgtctc tattgtcgtg tgggatctgg ctttccttct ttggtgagcc agtcgcatat
     2521 cgctatttcc ctccatcacc tacgatcacg cggacaccaa cgatcacgct ctccccgacg
     2581 atcacgcttt ctccgaccat cactctttcg ccaaccatta ccgatactcc atcgattaca
     2641 gacacttcaa cgattactcc cacgccgttc attcctcccg tgattgaagc catgttcgaa
     2701 agcgtagtga ctccaaatcc gaattcagtg ttcagcccac ttcaatttac tcgcaattgt
     2761 gatagcttta ccgagtttga ggctgccact gtgtttgata acccgatcac ttatatgtgc
     2821 gcggtcttta cctacgatca aatgacacct ggcgcgcaat ggactgcatt atgggtacgc
     2881 gatggcgaac tggttcatta cgaaacgatc ccctggaacg gcgaaacagg tggattcggg
     2941 ttcacggaat gggaagctcc tgctgaggag tggttgcctg gtacatacga agtgcaggta
     3001 tttgtcggat tggattggaa agttgtcggt cagttcatcg ttcagggcga tgctcctaca
     3061 ccacgaccga ctcaaactcc atctccgact cttacgccat cacgaaccgc aacggtaaca
     3121 aggactccca cggccacacg cacaccaact gcaacgcgca cgtttacccc cacgaggact
     3181 ccaactcccg ccacgccgac atcttctacg gttaccccat agctttgcat cgcgctgagc
     3241 ggagccatct gttctttgga tggcgcagac gaagcgcaaa taggaaatat ttcatgtccc
     3301 aaaagtcctt tcaagatcac tacccggatg tctttgctca ctgttacgga tgcggcaaac
     3361 ttaacgaaca cggtcaccag atcaaaagtt attgggatgg cgaagaatca gtttgtcact
     3421 tccttcccaa gccatattac atggcgatac caggctacgt ctacggcgga ttgctcgcct
     3481 cgctcattga ctgtcatggc acggggacgg ctgccgcagc gatgtataaa tccctgaaag
     3541 aaagcgatcc gaacacagaa cccaatacgc gctttctgac tgcatccctt catgtggatt
     3601 atctcaaacc gacacccctg ggcattgaac tcgaaatacg aggcagagta aaggaactga
     3661 aagggcgcaa ggtcgttatc gaagaatggc ttcttgccaa tggtgtggtc accgtacgcg
     3721 gagaagtgat cgctgtgcag gtgcccgagt caatggtgga ggagttggta aaggggaagt
     3781 aaaataatag agaaataatc gcgagcaggg aagttccgta tgtgcgtggc aaatcatact
     3841 tcattcgaga aggcacaata aaatgagtaa atctgtttac ataggcacgg atggcggcgc
     3901 gaccacttcg aaagttgggg gagtctggga cgatggttca acgatttcga caaaactgtt
     3961 gcaggcatca acgaattcca cacagggacc cgagtcggtg gtccggagtt gggtggacgc
     4021 tattgacgac tacttgtcca aaaattccct gacgtgggaa caggtccgcg gggtggggct
     4081 ggcagttcca ggtcccttcc aacgctatgg agtttttgat cactctgcaa acctgccaga
     4141 tagctttacc ggatttgacg ttcacactgc ctatcgtaac gcgctttcgg agcgtgccgg
     4201 gcgcgctatt ccactcaccg ttggtaatga cggagatatg ggtggcgtgg cagaagctca
     4261 acacgcgcgt ggcaatacaa cgggaactgt acttatgctt gccccaggct ccggattggg
     4321 ctgtgcgtac attgatgaaa atggaatcgc acttgatggt gatacgctgg ctggaatgga
     4381 agcgggtcac atgccggccc ttctacattt gctgaatgta aaagcctatc catgtggatg
     4441 tggacgtacc tggggctgta tcgaagtcta tacctgcctg gcaggcttac cccatctgct
     4501 cgccgaaaaa ttggagcagt atcctgagca tgacctggca aaatcatcgc tcagcatgaa
     4561 agaacgtgcc ttatcactgc gcggtttagc acagaagggt gaccagcttg ctacagagtt
     4621 gttcgacttc caggccaagg cattggggtt gcatgtggcc aatctcgcaa tggcgcttga
     4681 tcccaaatac gtcgtgatcg gcggcggatt gatggatccc gaagcgacaa ctattgactt
     4741 ccgcgaaaga tatctgaata tcgttcgatc aacggctcgc gagtatttat ggccctacca
     4801 acgcgatgtg atcagcatcg gacccgctgc acttggcgat ctttcacaag ccattggggc
     4861 ggcgttgtca gctttgtatc gggatagggc ttaattgcat tattaaggtg aatttaaaaa
     4921 aagacttccg cattctcaaa gaatgcggaa gtcttaattc actttttcat cggctttgcc
     4981 atttcatcct tcacggcatg atacaggttt tcagccttta gctcgcttag tgcgtaacac
     5041 ggcgctttga ggtgatttgc caattgttgt gcaaggcctt gatcaaacgc ggcgtgctcc
     5101 atattgatga ccacacattt gatcttttca tgtgcgatca attcagcgaa tttataagat
     5161 tcttcctgag ggggtagcgc accgatcgaa acattccctg ctccatctgt taaaacaatc
     5221 aagagaggtt ctacgtctgg atgatgcacc ttttcctgtc taagaacgtc agcagcgagg
     5281 aataatccag cggagagtgg ggtcttcccg ccgacaggga tatccatcaa tgcgcgctgt
     5341 gcaagttgaa cggaattggt cgggggcaaa acgagggtgg cgcggtcttt ctggaagacg
     5401 attaatccca cgcggtcacg gcgctgatac gcatcggtga gcaaggaaag aatggcgccc
     5461 ttcgtcgcgt tcattcgttc ggcgactgcc attgaccagg aagcatccac taagaacagc
     5521 acaaggttcg ctacgcgctt cacgcgtatc ttgcgctgca agtcggagga ctcgatggcg
     5581 aacgccagtc gttttctttt ttcttcgcgt tgtttctgga aaggggccgc tgcccgaaag
     5641 gtggcgtcga aggcgatgtc atctttcttt tcgccagcgg gtcgcgcctt gatataacgc
     5701 ccgtgcttgc gctcggtctt ggtcagcgaa cgccgacccg cgtgtttgcg tgccagtttg
     5761 tcgagcggag tatttaattt gcgcggctgg aacttatcgc caggcttgac cttttgcccg
     5821 ccttcccacc agttcgcaga tgtattcgcg aacacgtcct gcggcatctg ttcgggcttg
     5881 ccctcctgct gaccttcctc gcgctcatct tcgagcttta agtttttttt tcctcgcttt
     5941 ggccctgctg cgattcggac tcttgctccg cctcgctcgg tacagactgg cccgccaatt
     6001 gctcaatgcg ttcctgcaat tgctcggtgg tcatctcagc ctgttggaag ggagtccgct
     6061 tgatgcgatg ggggagagcc aactcggcgg caagcgcaat atcgtgatcg ttgatcttcg
     6121 tccgcccttc gaacgcagcc tgcgcgcggg cggctttgag gatcacaaga tctgcgcggt
     6181 gaccatctac attcaaggat gcggtcaacg cggcgatgga aagcaggtca cggcttgtgt
     6241 gagtcacctg cccgacgagc tcgcgtccgc gttcgatctg atgtgaaagc tcttcctctt
     6301 ttggcataaa cgtattgcgg aatgtgtcag catcctgttc gaagccgaga ttccgttcca
     6361 tgataaggac gcgttcacga gcactgcgaa tacctacgat atcgacagag agggcgaagc
     6421 gatctaatag ctgagggcga agttctcctt cctcaggatt catggttcca acgagaatga
     6481 agcgcgccgg atgcgagaac gaaatccctt cgcgctcgac catgttcacg cccatcgcag
     6541 cagagtccag caaaacgtcc acgacatgat cgtccaacag gttcacttcg tcgatgtata
     6601 gcagaccgcg attggctgaa gccagcacac ccggctcaaa gtgtcgttcc cctttttgaa
     6661 ttgcctgctc aatgtccagc gtcccaacca cgcgatcttc agtagcagaa accggtaaat
     6721 ttacaaaaga agtcgtcttc acatgaaatg gaagtgtatt attgttgcca ttggcgcgct
     6781 ccttacattc cgtgcaccag gtggcaggtt tgtcaggatc acagccgaaa cggcactcat
     6841 cgacaacttt gactttcgga agcagcgccg caagggcacg cgccgctgtg gattttgcag
     6901 taccgcgttc tccgcggatc aacacgccgc cgatacgggt atcgacagca tttaaaatta
     6961 acgcacgttt catgcgctct tgaccgacaa ttgcagtaaa cggaaaaatt gatggcattt
     7021 atatatgaac tccgagcggt gattataacg cgtgttccga atttccccaa atgaaagagg
     7081 gctcactcaa cacgaacttg aagattaatt atagcctgta aatggttcaa aatagtcgat
     7141 ttgggctatt aacagtaata cgggcaaagt gggttgtcaa cttctacaat cacctgtata
     7201 atccacatta cattttctca attggagtat caagtgacag atcagaatga ggccctgccc
     7261 gctgagggga acgatttaaa tcagcaggga gaacaacaca ctaacaacaa caatcaaacg
     7321 atggaatctt tgctgaaaga gcaggatctt aatgttgatt taccacaggc tggcgaaatt
     7381 cgcacaggcg tcattgcaag cgtcagcccc acccagatcc tcgttagcat aggcgccaag
     7441 tctgagggcg ttgtttctgg tcgtgaattg gatcagctat cgactgaaga acgcgaggcg
     7501 cttcaagtgg gccaggaagt gcatgtcttt gtcatcaatc ccgaagatgc aaatggcaat
     7561 gtcgttcttt cattgaagcg cgcccaggag caaatctcct gggagaacgt tgagaaattg
     7621 cttaccagcg ataacgtcat cgatagcacg atcatcggtt ttaataaagg cggattgatc
     7681 gtcggtgtag aaggtttgcg tggctttgtg ccctcctcgc agatcagcgc catgcgacgg
     7741 agtcaatcca caggcgagac gccagagcag cgctggcaga aaatgatcgg ccagcccatc
     7801 acggtccgtg taatcgaagt ggaccgcgaa cgacggcgcc tgatcctttc agagcgcgct
     7861 gcaagtactg agtcacgtca atcgataaaa gaacgcgtga ttgatgagct tgaagaaggt
     7921 aaagtatata cgggtcgtgt gaccagcctg gccaactttg gcgcttttat taatatcaat
     7981 ggtgctgatg gactcgtgca tctctctgaa ctttcctggg atcatatcga tcatcctcgt
     8041 gaagttttgg aggtcggtca ggagatcaag gtaaaggtca tcaatgtgga ccgcgagaag
     8101 aagcgaattg ggctttcggt tcgtgccttg cagagtgacc cgtggaaatc ccgcgtggaa
     8161 aaatatagcg tcggtcagct cgtagaaggt gttatcactc ggttgaccaa gttcggcgca
     8221 tttgcccgcc ttgagggcga tatcgaaggt cttattcaca tctcagagat tgccgaacat
     8281 cgtatcgagc atcccaagga ggtcctcaag gaaggtgagg ttaagagtct gcgtgtcatt
     8341 cgcatagacc ccgaacagca ccgcattggg ctgagcctcc gcaaagtgga ctcggctgcg
     8401 ttcgctgaca aggatttcaa attactcact gcagaatttg agacggagga tgaacccgaa
     8461 gccttcgaag cacctgaagc ttctgatgac tcaccagggg agtcaactga ggaaaacaca
     8521 gaagcttgat cggcaaaaaa gagcgcaccc caggtgcgct ctttcaccac tatgccccta
     8581 atcattgatt cacatcaaga ccttgcatgg aatatgctga cctatgggcg cgactatacg
     8641 cgctctgtgg tggagacgcg ccgtctcgaa accgaaactc tcataccgga ccaaaacggt
     8701 gactcaacca ttggctggcc tgaatatcaa cgtggtcagg tcgcagtggt attctcaacg
     8761 ttgtttgtga cacccgcacg caagaaacaa ccagcagata ccatttggta tacagatgct
     8821 aactcggctc atcgtcttta ccgtgaacaa attgacatct atcgccaact ggcggatgtt
     8881 catccagaca agtttcgcct catttcctcg acgaaggaac ttgattccgt tatcgagcac
     8941 tggaacaaac ccgtacaaga agacgaaggg catccggttg gcttgatcta tctcatggaa
     9001 ggcgccgaag ggattcgctc tccgcatgag ctcggcgaat ggtgggattt gggacttcgt
     9061 atgattggtc tggcttgggc agggacgcgt ttttgtggcg gcaccggtga gccaggtcca
     9121 ttgaccgccg agggacgtca actgctagct gccatggcag atcacaaatt tattcttgat
     9181 ctcagccata tggacgagtc ctccgcgttt gaatcattag atcggtacga gggtccggtg
     9241 atggcttctc atgccaattg cgcggcactt atgcaaggag cagagacaaa tcgccatttg
     9301 ccagaccgtg ttatccgcag cctaattgaa cgagaaggga taatcggctc gattccattc
     9361 aatacgttcc tcaaagtagg ctggaagcga gaccggggta gtcgtcgtga agaggtgcct
     9421 ctggatgttt tgattgctca ccttgatcac atatgtcaac tggcggggaa ttcgctccat
     9481 gcgggcattg gctcagattt tgacggtggc tttgggctgc aatcgattcc ccctgaactg
     9541 gactcgattg ctgacttgca attaatcgcc tctaaattaa ttgcccgtgg atacagcgag
     9601 ctagatgcag aaaacatttt gggtggcaat tggctgagat ttcttcggaa gcatttgccc
     9661 tcatgagtga agaacccata cttcgaatct ctaatttaag aatccgtttt agcctcgcca
     9721 acgtatttct gggctttgtg atatttgcta ttggagccaa gccgaattgg tttggctggg
     9781 atcggagccc agtggtgggc tttatacaaa ttgcggtttt ccttgtggga ttggctttga
     9841 tctcggcggg tggatacgta ggcctgcttg cgttatgggg aaatgaacag cgcagcattc
     9901 ttgccgatat tggttcgcgc ctggttggga cgggttacgt tattgcgatc tttgcgggca
     9961 tggcagatgt ctttggcctt ggaacgcagc ccttaccaga agtgccctat tttggtccgt
    10021 ggcaggcagc tggcgtgttc attggagaat tggttatcat tgcggggttt ttgatgatca
    10081 ttccttatcg gtttgagggg aagaaaagac ctgaaagtac aaatccataa atttggaaga
    10141 ttttcctcaa ggacttaaag attaaacgag tcacgtgctg tggatgcgaa cacgtgactc
    10201 gcttttcttc tacagtatct aatcgcgtta catcatctga acttccacaa ttcgactcct
    10261 gcgctatcac cgccatcggc ccggaagtaa agttcattgt tataaactgc caaatatgcc
    10321 gggcttgagt taccacctgg attgatatct gcggctcggg tagcggtggt cccgtcgaat
    10381 ttccataact caataccagc attatcactt ccggctgcgc caaaatagag ggctccgttg
    10441 aaatcagtca aaaacgtggg cgccgaatcg ccggcccctg tgttgatatc agctgcacgt
    10501 gtggggggat tcgtgccgtc atatcgccat aattctgttc cggtgccatc gttggcgttt
    10561 gcgctaaagt acagcatgcc gccaaatcct tccaaatatg ccgggtttga attacccact
    10621 cctgcattaa tatcagatac gcgtgtggca gtggtgccat cgaacttcca taactccgtt
    10681 ccggcgccgt cgttactggt cgcgctaaag tacagggcat ccttaaagac tgtcagataa
    10741 gcaggacttg aatcgccact gccattatgg atatcggcgg cgagcgagga tgtggtgcca
    10801 tccgtcttcc agagttccac gcccttgcca tcattgccat tggcactaaa atacaatgca
    10861 ttattgaata ccgctagatg agatgggttt gaatcgccag caccaggatt gatatcggtc
    10921 aagcgacccg tagccgagcc gttgtatctc cacaattcga cgccagcacc atcgttccca
    10981 ttggctttaa aatacagggc accattaaac tccgtcagat aggatggatc ggagcctgtg
    11041 gctccagcat tgatatccgc gacgcggctg gcggcgctta atggtccttc gaatttccat
    11101 aattctgatc cggcattgtc attgcctacg gccgagaagt acagtgttcc attgaaaact
    11161 gccaaatgag cagggtcaga attcccagct ccgggattga tgtcagcgac gcgtatagct
    11221 ccggttgccg gggggggtgt cgtcggtggc ggtggcgtgg aaagggtaac tttcaggtta
    11281 taaggtttgt tcgatgccac cagtacactg acgagttcaa taatatagtc accggtggta
    11341 gggattgttc cagtccaggt taatgttgta tcctgcgcct tgagtgcgct accgttgggt
    11401 tgataaattg ccaaagccaa ttcgttcgtg ggagcgcccg tcacttcgat gctcaacgtt
    11461 tgacccgctg tagcgttcaa cacgtagcga acttttcctg ctgctgttac agtgccaggc
    11521 aaggtcacag tgttcgttcc cgctgggaag gtaatgcggg tcgcatcggg ttgcggcgtg
    11581 acgggaacag tactgcgtgc cgaatttctt ggaatcttga gttcgacgcc gttcgacacg
    11641 gtctttccga cgtttgctgc ccgcaggact tcaagatctg cattgaactg tctcgcgatg
    11701 gattcccatg tatcacctgc ttttactgta tagaacacca cgcaatctac tgggccgtaa
    11761 atgggaccat cgctgccgat atttggaacc acgagttccg gtgtctttgg gaatgggaag
    11821 attaagtccg catcggtcac acctggattc gctaaccgta ccgcctcaaa atttgcgccg
    11881 tagcaacgcg caatctgaag catccactct ccttttgcaa ctttgtgacg aatggtagag
    11941 ccacgcggaa ggttcgaggg tgtccctggt ggaggcacgc cgttcgtgat cgtgacagtt
    12001 gccggattga tagaactcgc gccggccccg gaggcagttg cactatttgt gatttgggca
    12061 acggccatat cgttttgcgt gatggtgtag atcgccgtgc agcttacagt ctggttcgtg
    12121 gctaaggtcg tagttgccac accacaattg atcggcgtag tgatgtgatc atcgcgaaca
    12181 acaaattgag ccggacccaa tgttgtggcg cctgtattgt ttatagtgta agtgtaggta
    12241 atcgtctggc cggccgcgct gtatgaagtt gggctggcag atttagcaat tgtcaaaact
    12301 ttattttctg ccatacggac cgtagccgat gcaagcgcag atgtcactcc acccacactg
    12361 gctgtggcat tgtttgttac ctgacctgcg ttgaggtcat tttgagtaat ggcgtatgtt
    12421 cctgtgcatg aaatgctttc attgaaatca agattattat ttagattgcc cacggtattg
    12481 acattcgggc aggcaacacc agccacttta tcatcggtga tcgttacagg acctgg
//
LOCUS       DASTJP010000290         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1095300, whole genome shotgun sequence.
ACCESSION   DASTJP010000290 DASTJP010000000
VERSION     DASTJP010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095300"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1562)
                     /locus_tag="VFQ23_08755"
     CDS             complement(81..1562)
                     /locus_tag="VFQ23_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906719.1"
                     /translation="MPDKDEKKPEEKKEEKTGAVDRLVESSHSIRIKGREVKYTVTTG
                     TMVLKEETADRGKEAEGEKPRAQIFFIAYTKEGVKDKSKRPLTFSFNGGPGSSSVWLH
                     LGVLGPRRVLMKDDGELPPPPYKLTDNEYSLLDETDLVFIDPMNTGYSRPVEGTKPQE
                     WHGFKKDIESVGDFIRLYTTRTNRWLSPKFLIGESYGTTRAAGLSGYLQDRHGMYLNG
                     LMLISVVLDFTTLDFNINNDLPFILYLPGYTATAWYHDLLQPHKPLQTWLKEAEEFAL
                     GEYANALLKGDSLSKEERAQIVEKLSYLTGISTQFIERSNLRINDQHFFKELLRERGR
                     TVGRLDSRLIGRDRLGITENPEYDPLLTNIMGPYTAAFYEYVRSELKFESDLPYEILS
                     GFVHPWSFSEFENRYVNVGETLRAAITHNPYLKVFVANGYYDLGTPYFATEYTFNHLG
                     LEEELRKNISMEYYDAGHMMYIHIPSLKQMKTDLTNFIRGAVA"
     gene            complement(1573..2397)
                     /gene="rsmI"
                     /locus_tag="VFQ23_08760"
     CDS             complement(1573..2397)
                     /gene="rsmI"
                     /locus_tag="VFQ23_08760"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET9906720.1"
                     /translation="MGTLYLVATPIGNLEDMSPRAVRVLREATLIAAEDTRNTGTLLK
                     HFEIKTPITSYFEHNKLNKLDFILEKLSTGDVALVSDAGTPAVNDPGYELIRAALASN
                     FDVRPVPGPSAPIAALTASGLPTDSFLYLGYLPHKTSDRHKFIGQVANLSYTLIFLES
                     PYRIVEALEDIFSILGDRRICVAREMTKMFEEFWRGNVSGAVEHFKSQPARGEFTLVI
                     EGRKGETNEKWTEQQMIKAIETEMRNGKSAKDISSELAIQSGWSKKEIYSLVNQRK"
     gene            complement(2450..>3599)
                     /locus_tag="VFQ23_08765"
     CDS             complement(2450..>3599)
                     /locus_tag="VFQ23_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906721.1"
                     /translation="SVGWYLIYYASELKQYSSDVMIALLLVYLARKCLQEQAQARDFV
                     VLGISGAIAIWVSHPSVFVLAGVGLVLVIDKVTRKSEIPFAWILIMGFLWVGLFAIDY
                     FVFLRPLAADEYLQNYWGKAFMPLPPWSDTGWFKTTFYSMLLMSLHTYFSAVVTVSIL
                     LVIGGISLLFRNRNMALLFILPALIVLMASGFEAYPLKDRFMLFLVPFFLLILAEGLR
                     AIYTFAAKWSRPLGVVLATVPALILIWPPGTTLDQFFITTRGSDVRPVIQYVGTNRKP
                     DEIVYVFHAGESAFQYYAPLYGLNTGEVIFGPSSLRKKFVLDGFFNDVENLRGKERVW
                     FIFSNIYDCGDCEGNMQQFYVDYLNGFGIMLDQVNAADANAYLYDFNP"
BASE COUNT          972 a          886 c          833 g          908 t
ORIGIN      
        1 tgcaaggtta caaatttgtc aggcaaaagg ttacaaataa aggaattctg aacaggaagt
       61 gtgatttgcg ctatttttgc ttatgccact gcgccccgta taaaattagt caaatctgtc
      121 ttcatttgct taagtgacgg gatatgtata tacatcatgt gaccagcgtc gtagtattcc
      181 attgaaatgt ttttccgcag ttcttcttcc aaccccaggt gattgaaggt gtattccgtc
      241 gcaaagtaag gtgtccccag gtcataataa ccgtttgcca cgaagacctt gagatagggg
      301 ttgtgagtga ttgcggcacg cagcgtttcc ccaacattga cgtatcgatt ctcaaattca
      361 gaaaatgacc agggatgaac aaaaccgctc aaaatttcat agggcagatc agactcaaac
      421 ttcaactcac tgcgaacata ttcgtagaag gcagccgtat aagggcccat gatattagtc
      481 aataagggat cgtattcagg gttttccgtg atgcccagtc tgtcgcgccc aatcagacgg
      541 ctatcgagtc gtccgacagt tcggccgcgc tcgcgtaaca gttccttgaa gaagtgctgg
      601 tcgtttatac gcaggttcga ccgttcgata aattgagttg agataccggt caaatacgag
      661 agtttttcaa cgatctgtgc acgctcttcc ttgctaagtg aatccccttt caacaacgca
      721 ttggcatact cacccaaggc aaattcctcc gcttctttaa gccaggtttg tagtggctta
      781 tggggttgga gcaaatcatg ataccaggct gtcgctgtat aaccgggcag atacaggatg
      841 aaaggcagat cattattgat attgaaatcc agcgtggtga aatccagcac caccgagatg
      901 agcatgagtc cgttcaagta cataccgtgc cggtcctgca aatatcccga aagtcctgcg
      961 gcacgtgtag tgccatagga ctcaccgatc aggaatttgg gagaaagcca gcggttggtg
     1021 cgtgtggtgt acaagcgaat aaaatcgcca acagactcaa tatccttctt gaagccgtgc
     1081 cattcctgcg gcttggttcc ttccacggga cggctatagc ccgtgttcat ggggtcaatg
     1141 aataccagat cggtttcgtc aagaagtgaa tactcgttat ccgtcagctt gtagggaggt
     1201 ggaggcagtt ctccatcgtc cttcatcaaa actcgacgcg gccccagaac acccaaatgc
     1261 agccagacgg aggatgaacc tggtcctcca ttgaacgaaa aggttagagg acgtttggat
     1321 ttgtccttca cgccctcttt tgtataagca atgaaaaaga tctgagccct gggtttttca
     1381 ccctctgcct cttttccgcg atcggctgtt tcttccttaa gaaccatcgt gcccgtcgtg
     1441 accgtgtact tgacctctct gcccttgatg cggatagaat gactggattc aaccagccga
     1501 tcaaccgccc ctgttttttc ttcctttttc tcttcaggct ttttttcgtc tttgtcgggc
     1561 atgacgtgct ccttatttac gttgattcac cagcgaatag atttcctttt tgctccagcc
     1621 gctttgaatt gccagttcag aagaaatatc ttttgcagac ttaccatttc tcatttctgt
     1681 ttcaatcgcc ttgatcattt gctgctcggt ccacttttcg tttgtttcac ccttcctgcc
     1741 ttctattaca agagtgaact cccctcgcgc gggctgggac ttaaaatgct ccaccgctcc
     1801 actgacgttt ccgcgccaga attcctcgaa catctttgtc atttcgcgcg ctacacagat
     1861 tcggcgatct ccgagaatag aaaaaatatc ttccagcgct tcaacaatac gatatggcga
     1921 ttcgagaaaa atgagcgtat acgataaatt agcgacctgc ccgatgaatt tgtgacggtc
     1981 gctggttttg tgaggaagat atcccaaata gagaaatgaa tccgtcggca agccggaagc
     2041 ggtcagagcg gcgatcgggg cggaggggcc gggaacaggc cttacgtcga aattcgaggc
     2101 gagggcggcc ctgatcaatt cataacccgg atcattcaca gccggagttc cggcatccga
     2161 gacgagcgcc acatcccctg ttgatagctt ctccaggata aagtccagtt tgttcagttt
     2221 gttatgttca aagtagctgg taataggggt ttttatctcg aaatgtttga gcagagtacc
     2281 tgtgttacga gtatcctccg cggcgattaa ggttgcctcc cgcaaaaccc gaactgcacg
     2341 tggcgacatg tcttcaaggt taccaatggg agtggcaacg aggtaaagcg tacccattac
     2401 aagattgtat cacttcgtca caggctatta tttttcgagc aggtaaacct tatgggttaa
     2461 agtcatatag gtaagcattg gcatcggcag cattgacttg atccaacatt atcccaaacc
     2521 cgtttagata atccacgtaa aactgctgca tattgccttc acaatcaccg cagtcgtaaa
     2581 tattcgaaaa gataaaccag acccgttcct tccctctaag gttttccaca tcgttaaaaa
     2641 agccatccaa gacgaacttc ttccgcaggc tgcttggtcc aaagatgacc tcgccagtgt
     2701 tgagaccata caggggagca taatattgaa aggcagactc acccgcgtgg aacacgtaca
     2761 caatttcgtc tggctttcta tttgtcccga catattgaat gacaggccgt acgtctgagc
     2821 cgcgggtggt gatgaaaaac tgatccagcg tggtgccggg gggccaaatc aaaatcagcg
     2881 ctggcacagt agcaaggaca acacccaaag gacggctcca tttcgcagca aacgtataga
     2941 tggctcgcag cccttctgca agaatcagca ggaaaaatgg aaccagaaat agcatgaatc
     3001 ggtctttgag aggataggct tcaaagccag aagccatcaa aacgatcaag gcaggcagaa
     3061 tgaaaagcag ggccatgttt cgatttcgaa ataagagaga tatcccgccg ataaccagca
     3121 aaatggagac ggtaaccacc gcgctgaaat atgtgtgcaa actcatcaac aacatggaat
     3181 agaaggtcgt cttaaaccaa cccgtatcac tccatggagg cagaggcatg aaggctttac
     3241 cccagtaatt ttgcaggtat tcatccgcag ccaacggtcg cagaaatacg aagtagtcta
     3301 tcgcaaatag accgacccaa aggaagccca tgataagaat ccaggcaaac ggaatttcac
     3361 tttttcgcgt tactttatcg atgaccagca ccaatccgac tcctgccagc acaaaaaccg
     3421 atgggtgaga tacccatatt gcaattgccc cactgatacc caaaacaacg aaatctcttg
     3481 cctgagcctg ttcctgaagg catttacgtg cgagatagac caaaagcaac gcgatcatga
     3541 catcgctcga atattgctta agttcggagg cataatagat cagataccat ccgacggaa
//
LOCUS       DASTJP010000291        15723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1420692, whole genome shotgun sequence.
ACCESSION   DASTJP010000291 DASTJP010000000
VERSION     DASTJP010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420692"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..873)
                     /locus_tag="VFQ23_08770"
     CDS             complement(115..873)
                     /locus_tag="VFQ23_08770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906722.1"
                     /translation="MYERDLEKAALRGEPSYVWRAGQQRRFEMILKSAGDRIHGHILE
                     NGCGVGMYLEHLSAFGGKVIGLEYDFERAAQAKTNSPHIINAAGESLPIHSSTFDLIL
                     SHEVIEHVQDDRAAIREMVRALRSPDPASGKPGGRMILFCPNRGYPFETHGIYWKGKY
                     QFGNKLFVNYLPRLLRDKLAPHVRVYSTHDLLKLFDGLPVKFIEKTIIFGAYDNIIAR
                     FGVFGTFLRGGLQFLEKTPLKIFGLSHFWVLEKI"
     gene            1010..2911
                     /gene="nusA"
                     /locus_tag="VFQ23_08775"
     CDS             1010..2911
                     /gene="nusA"
                     /locus_tag="VFQ23_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET9906723.1"
                     /translation="MSKNEFSLAFNEVLEEKQLSKDVILNAIESAMVSAYRRAVNAST
                     AQHVEAKIDSDTGKVLVFAEKEVVEDILEPKTEVLLEEARKVNPEAQLGDMVVVETTP
                     ADFGRVAAQTARQVIQQRIREAERSNQMQYFERQVGEIVSGIVQATNAQATTVGLDMK
                     AEGVLPGNQRIPGERFKVHDRVRAVVLEVKDGPRGPQIVLSRAHRNFLRRLLENEVPE
                     IYHGVVEIRAISREPGARAKVAVMATQAGVDPVGACVGIKGVRIQAIVKELHDEKIDI
                     IQWDQDTVVYISKAISPARVSGVYLSEVEGNRTATVVVNEDQLSLAIGRDGQNARLAA
                     KLTGWRIDIKSLAEAAGDAIQKLRVDLELAALLPNAVESIPAVEEVLTKKAENRPITP
                     EEFTMLGQFVDRVERRTIQIKEDAARAAEEKTASARAEVPTAAFGMPLEEIGLKEHVF
                     NILVEAGFDTVGALMMSMKMDANRVLGLPGIGPKAMQNIEETLAALTFPEPAPVEPVV
                     EPLVEAVAQVEVQPEASAPVAVAEPVVVEEIPAPEKPVVPKKEPRKVVEEEDDEHAKD
                     GVSLDELFAMKPEIFQSAAGEEEDAADKKKGKKGKKKSVELVLDEERGEVIGRKKHKR
                     GDLDFGDDE"
     gene            2960..3274
                     /locus_tag="VFQ23_08780"
     CDS             2960..3274
                     /locus_tag="VFQ23_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET9906724.1"
                     /translation="MSKKPVQRVKHVPQRTCVGCREVLPKRKMIRIVRTTDGVRVDPT
                     GKLAGRGAYLHDRRDCWARGLKGSLAHALKTTLTSDERARLEDFMNALPADAEAELAG
                     GG"
     gene            3375..5147
                     /gene="infB"
                     /locus_tag="VFQ23_08785"
     CDS             3375..5147
                     /gene="infB"
                     /locus_tag="VFQ23_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET9906725.1"
                     /translation="MSINGNKIELPTSIVIRDLAQKLEKSPIDLIKKLMSNGVMATIN
                     QSVDFDTAAIVMAEYGYEAVPEVVEEVKVEVGEVPLWRQLIAGEDQLLLKNRPPVVTI
                     LGHVDHGKTSLLDAIRQTEVAAGEAGGITQHIGAYQVEMKGRQITFLDTPGHAAFTQM
                     RARGAQGADIVILVVAADDGVMPQTREAIAHAKAARVPIMVALNKIDKANANPDRVKQ
                     QLAELELVPDEWGGNTMIIPVSAKLKQGIDDLLEGILLVADNIEIKANPGGKVIGTVI
                     EAEVDKTKGVMATLLVQNGTLEFGDIVVAGIAHGKLRALSDYRGKPVKKASPSTPVAV
                     MGLSDVPSAGDLFEVVDSEREARSIVAERAEAAKSQAQAKKKVSLEDLFASVQAGEAK
                     ELNLIVKADVQGSLDPIVSELNKLGEGQIGIKILYSETGNIGENDVMLASASNAIIIG
                     FNVQADVSARRLAEKEGVDIRLYEIIYRMTEDIEKALNGMLEPEVKEKIIGRAQVLQV
                     FSASKFGKVAGCRVTDGELRRNAKVRLFRGTDLVFEGDMGSLRHEKDDVREVKQGYEC
                     GVGFKNFSDIQSGDQLICYILETS"
     gene            5177..5527
                     /gene="rbfA"
                     /locus_tag="VFQ23_08790"
     CDS             5177..5527
                     /gene="rbfA"
                     /locus_tag="VFQ23_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9906726.1"
                     /translation="MPSGTRLQRIADRIRQELSEMLIREISDPRLKQIYITDVKVDKE
                     LAYADIFVSAIEGVSRSKDILAGLESASGYVRRTLASRVELRAFPRLRFHWDMTPENS
                     DHIEKILAELRAKK"
     gene            5576..6535
                     /locus_tag="VFQ23_08795"
     CDS             5576..6535
                     /locus_tag="VFQ23_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET9906727.1"
                     /translation="MDQASNAIKARLEQATNIIIASHVRPDGDAIGSLLGLGLALQNA
                     GKSVQMVLVDGVPASYRHLEGSETIVKQPGGEHDTFITVDSADFRRVGKVFENFGQPD
                     INIDHHKTNEKFGKLNLIEAEEVATAAILTNHLREWGYEITKPIADALLTGIITDTLG
                     FRTPSTNPSALRLCATLMETGADMPDLYMKSLVRKSFPAARYWGAGLSSMEEKNGIVW
                     GTLTVEDRKKAGYGGNDDADLINMISAIDGNKVGMIFVEQTDNHVKISWRALEPGIDV
                     SPIAKHFGGGGHAAAAGADIPGTLQDIQPLVLKTTYEMLGFER"
     gene            6594..7520
                     /gene="truB"
                     /locus_tag="VFQ23_08800"
     CDS             6594..7520
                     /gene="truB"
                     /locus_tag="VFQ23_08800"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET9906728.1"
                     /translation="MNSQDVKNAISGVLVVDKPVGMTSHDVVQAIRNGTGLRRAGHTG
                     TLDPRASGVLVILVGPAVRLSEYVSASDKRYQAIIRLGGRTDTFDADGKVTQATQPVN
                     VTESQFEEVLKTFVGEIEQTPPPYSAVKVQGRKAYEMARKGEEVELEPRKITVHHLEV
                     LEWAPPEVVIDVHCSSGTYVRSLANDLGEKLGCGAYLVGLRRTKSGRFSLRDAVPLRK
                     LQESFHAGNWYQYLIPAAEALGDWPALELSPDDVEGVRHGHRVTAAADAVIGSRVRGV
                     STQGELVALMECVTGEDGSPAWQPKKVFFTSE"
     gene            7623..8543
                     /locus_tag="VFQ23_08805"
     CDS             7623..8543
                     /locus_tag="VFQ23_08805"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET9906729.1"
                     /translation="MKHYHSLEEVSLENSWLTVGVFDGVHRGHQEIIQKLTNDAHANG
                     NPAVVLTFHPHPANVLGRGEIGLLTFPAERAELLASMDVDVVITEHFTRELSSVTAFD
                     FMSRLKRNLGLKQLLIGYDFALGKGREGNAPRLTEIGGELGYTVEVVSALSDESGVIS
                     STEIRKLVSVGNVAEASHLMGHAYSLGGPVVRGDGRGKGIGVPTANIEYPREKIVPAK
                     GIYAGWAHLDGERHKAAISIGVNPTFTPEKQIPSVEAYLLDFDRDIYGKDLYIEFVAR
                     LRDELKFDSVDALVEQIWKDVEEARSILGS"
     gene            8637..9191
                     /locus_tag="VFQ23_08810"
     CDS             8637..9191
                     /locus_tag="VFQ23_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906730.1"
                     /translation="MTQSFNQNPEPLQPLSAEGSPPFNREAEIAEITQRMKRGANNFY
                     WIAALSVINSILSMTGSSTYFVIGLAITLVTDGITIGLSQSLPDAAVIVKIVGLVLSV
                     LIAGVFALFGYFASQGKRWAFLVGMVLYALDGLIMLAFADWIGVLFHAYFLWGLFGGW
                     RALGELQQKSSQNQMAFPQDIGVQ"
     gene            9260..10699
                     /locus_tag="VFQ23_08815"
     CDS             9260..10699
                     /locus_tag="VFQ23_08815"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET9906731.1"
                     /translation="MPEREIYLLSPRALSPETIAVAFAKTSRSPESFRDIAAELSDEK
                     SAQFHEKWVVGYGHASVAEHAVLHIAFENVSRLAIESIEGNRLASYTEKSTRYQKWGS
                     DDFTVPPELTDHQLRDEFEDTVRLLFRTYAESLDPVRNLILERFPRRENEKDEAWDRR
                     IRSKYVDVCRFLLPAAALANVGMTANARVLENMIRKMLSHELAEVREIGARVKEVAKS
                     EAPTLVKYADAVPYLMETMNEFSHRDAESQSIKKSPRLSDSVADQWCTLVDYDQDGEE
                     NVFAAALYRFGEMGYADALSYIKTLGEPEREKLAEILLARLGKFDVPLRELEYCTYTF
                     DLVMDQGAYAEFKRHRMMTQTPQKLTTRLGYATPRLVTEAGFESQYEAAMEAASQMFE
                     TLYQFNPNVAQYIVPNGFNRRVLAQFNLREAFAFCQLRSAANAHFSIRRVAQRVYEEM
                     ARVHPLLTRYMKLHDETWQGVEELHFTGM"
     gene            complement(10756..12117)
                     /locus_tag="VFQ23_08820"
     CDS             complement(10756..12117)
                     /locus_tag="VFQ23_08820"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET9906732.1"
                     /translation="MATKIIFPENFIWGAATASYQIEGSWDKHGKGESIWDRFSHTPD
                     KIHNGDTGDVANDHYRLWKKDIRIMKQLGLKAYRFSTAWPRILPKGRGKVNQKGIDFY
                     SKLVDGLLEANIIPFVTLYHWDLPQVLQDEGGWEVRSTAEAYVEYTEAISKALGDRVK
                     NWITHNEPAVVSWIGNDTGEHAPGFKDLAKGVAVSHHLLLSHGWAVPVLRRNSPDSEV
                     GITLNVGWRVPASNSSADLNAVREGDGKWTRWFTDPLYGRGYPADVVADFERQDAMPN
                     QMSFIKNGDMKSISVPLDFLGVNYYNREVVRSVASDNETQVVFSKPKTPENWTEMGWE
                     NFPEGLTGVLSRLYFDYKPLKMYVTENGASYSTPPDETGRVKDDLRIDYYRTHIAAVH
                     RAIQAGVPMAGYFAWSLMDNFEWARGYAQRFGLVWVDYETQKRILKDSAKWYRNVIKK
                     NGF"
     gene            complement(12171..14156)
                     /locus_tag="VFQ23_08825"
     CDS             complement(12171..14156)
                     /locus_tag="VFQ23_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9906733.1"
                     /translation="MSTNAMKRIFYFLFLISLTVASCTGESVTPAPVTEPTTIANDEF
                     GPVIYTDSSQPVEARVEDLLQRMTMEEKIGQMTQVEKNSIRAGDITRYYIGSILSGGG
                     GAPKENTAQGWYAMVEAFQDEALATRLEIPLIYGVDAVHGHGNLLNATVFPHNIGLGA
                     ANNPELVEKIGRATAEEMLASGIPWNFAPVIAVVQDVRWGRTYEGFSENTEIVSSLGT
                     AYIRGTQQLKDNDEASAGQSLFALATPKHFIGDGATIWDSSRTEDYRLDQGNMQVPET
                     ILRELFLPPYQAAVEAGAMNVMASFSSWKGIKLHAQQPLLTDILKEELGFNGFIVSDW
                     QAINQIYPDDYYSSVVTSINAGVDMNMVPYEYVAFIETMQKAVTNGDIPESRIDDAVR
                     RILRVKFALGLFEQPMPDTKYQGTIRSREHLELARQAVRESLVLLKNDNNVLPLSKET
                     PSIFVAGEAANDIGLQSGGWTLEWQGKPGDDNEGTTIFSGIRAVADSDAQVLFNRDAD
                     FSEFKDGDGNQLIADVGIVVLAERPYAEGVGDQADISLNDAEIQLIMEAREQSKNLVV
                     ILLSGRPRVITEQLPLAEAWVAAWLPGTEGGGIADVLFGDFPFTGKLSYSWPRSNEQL
                     PININNSADKTGCDAPLFPFGYGLTYQEPNPEILECP"
     gene            complement(14166..14861)
                     /locus_tag="VFQ23_08830"
     CDS             complement(14166..14861)
                     /locus_tag="VFQ23_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906734.1"
                     /translation="MSLVIETKDLRRVYPSKRNEEGIQALRGVNLNVESGSFVTLKGR
                     SGSGKTTLLNCLAGLDKPTSGTAIVLGNDLMQMSDDELTRWRREQIGLVFQSFGLLPT
                     LSAYENIELLLRIKGDEYEARHQRALQCLDLVGLGKWRDHRPYEMSGGQQQRVAIARA
                     LANHAKLILADEPTGELDSKTTRELLTFFRELVETQHITMLMVSHDPLVDQYVHQVLT
                     LKDGVIENHMGRG"
     gene            complement(14858..>15723)
                     /locus_tag="VFQ23_08835"
     CDS             complement(14858..>15723)
                     /locus_tag="VFQ23_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9906735.1"
                     /translation="GSGKSTLLNILGGLDRPTGGRVLVERQDLGSFSERALDAYRLEQ
                     VGFVWQQATRNLIPYLTALENVELPLTLSGQVGNSSRVRATDLLKIVGLSERADHHLE
                     ALSGGEQQRVAIAIALANRPKLLLADEPTGELDTATAKSIYDLFRALNNDLGLTIVIV
                     SHDPGIAQHVDRVVAVRDGKLASETLRVQKSDSDEHHLVELAVLDSAGRLQIPREYLE
                     QFQINRRVQIEMTEEGILIRPPEGNHHGHTQSFSAGEEELPESEDVQLPSGWSRIWQS
                     VKSRFARGEKE"
BASE COUNT         3932 a         3724 c         4138 g         3929 t
ORIGIN      
        1 cgagtgaaaa aatctttagc agagattttt gtgaaccaat tcaaaacacg gacgttcgag
       61 tctttgagcg cggttattag ctgagatttt gttgagactt caatcatagt gtccttaaat
      121 tttttccaac acccaaaaat gagataaacc aaatatcttc aacggtgtct tttcaagaaa
      181 ttgcaaaccg ccgcgcaaga atgttccaaa aacgccaaat ctagcaatga tgttatcgta
      241 tgccccaaaa atgatggtct tttcaatgaa cttaactggc aagccgtcaa agagtttcaa
      301 tagatcatgc gttgagtaaa ctcgtacgtg tggagccagt ttatctcgta aaagacgcgg
      361 taaataattc acaaataatt tatttccgaa ttgatatttg cctttccagt aaatcccgtg
      421 ggtttcaaat ggataaccgc gattcgggca gaagaggatc atgcgtccgc caggcttccc
      481 cgacgccggg tcgggactgc gcaatgcgcg gaccatttcg cgaatggcgg cgcggtcatc
      541 ctgaacgtgt tcgataactt cgtgactgag gatcaggtca aatgtcgagg aatggattgg
      601 aagagattca ccagctgcat tgatgatatg tggagaatta gtctttgcct gtgccgcacg
      661 ttcaaagtca tattccaacc cgattacttt acctccaaat gcggagaggt gttccaaata
      721 cattccgaca ccgcatccgt tttccagaat atgcccatgg atgcgatctc cggctgattt
      781 taagatcatt tcgaatctgc gctgctggcc ggcccgccaa acataggagg gttccccacg
      841 taatgcggct ttttcaaggt ctcgttcata catatttgga attatatctt gcaactttta
      901 attgcctatg ctagaatatc aattgtagtt tgagttgagt accgcacgca tcgagaagtg
      961 ggcgatcccc acttttctgc ttgtaaatag gagtatacgg agtaagagca tgtcaaagaa
     1021 cgagttctcc ctggctttca acgaagtgtt ggaagaaaaa caactttcca aggacgtaat
     1081 tctcaacgcg atcgagtccg cgatggtttc ggcttatcgg cgggctgtga acgcgtccac
     1141 tgcccagcat gtggaagcca aaattgactc ggataccggc aaggtcctgg tctttgctga
     1201 aaaagaagtt gtagaagaca ttctggagcc aaaaaccgag gttttgctgg aagaggcgcg
     1261 caaggtcaat cccgaggctc aactcggtga tatggtagtc gtcgaaacga cacccgccga
     1321 tttcggtcga gttgcggcac aaacggcgcg tcaggtcatt caacagcgca tccgtgaagc
     1381 ggagcgctcc aaccagatgc agtatttcga gcgacaagtg ggtgagatcg ttagtgggat
     1441 cgtacaggcg accaatgccc aggcaacaac agttgggctc gacatgaaag cagaaggggt
     1501 tttacctgga aatcagcgaa tcccgggtga gcgtttcaag gtgcatgatc gtgtccgtgc
     1561 ggtcgtgctt gaagtgaaag atggtccgcg tgggccgcaa atcgttcttt ctcgtgccca
     1621 tcgcaacttt ttgcgacgtt tgcttgagaa tgaggttccc gaaatttacc acggtgtcgt
     1681 tgaaattcgc gcgatctcgc gtgagccggg ggcgcgtgcc aaggtagcag tgatggcaac
     1741 tcaggctggc gttgaccccg ttggtgcctg tgtgggcatt aagggggttc gcatccaggc
     1801 aattgtcaaa gaattgcatg atgaaaagat tgacattatt caatgggatc aagacaccgt
     1861 tgtttatatt tccaaagcga tcagcccggc gcgcgtgagt ggtgtctatc tctccgaagt
     1921 ggagggaaat cgtacggcga ctgtggtggt caatgaagat caactcagcc tcgcaattgg
     1981 acgcgatgga caaaatgctc gtctcgcagc aaaacttacg ggctggcgta ttgatattaa
     2041 gtctctggcg gaagcggctg gggatgccat tcaaaaattg cgagtcgatc ttgagctggc
     2101 cgcgcttcta cccaatgctg tggaatcgat ccctgcggtc gaagaagtgt tgacgaaaaa
     2161 ggctgagaac cgtccgatca ctcctgaaga atttacgatg ctggggcagt ttgttgatcg
     2221 tgtggaacgc cggacgattc agatcaagga agacgcggct cgtgctgcgg aagagaaaac
     2281 agccagtgcg cgtgccgaag tgccaacggc tgcgttcggt atgccgctcg aagagattgg
     2341 tctcaaagaa catgtcttca atattctggt tgaagcaggg tttgatacag ttggcgccct
     2401 gatgatgtct atgaagatgg atgccaatcg agtgcttggt ttgcctggga tcggtcccaa
     2461 ggcgatgcag aatatcgaag agacgcttgc cgcgctgaca ttccccgaac cagcccctgt
     2521 ggagccagta gtggagcctt tggttgaagc ggtcgcgcag gttgaagttc agcctgaggc
     2581 atctgctcca gtagcagtag ctgagcctgt tgtggttgaa gaaattcccg cccctgaaaa
     2641 gccggtggta cctaagaaag agcctcgtaa agtcgtcgaa gaagaagatg atgagcacgc
     2701 caaggatggc gtctcattgg atgagctctt tgcaatgaaa ccggagatat tccaatctgc
     2761 tgctggggaa gaggaagacg ccgcagataa gaaaaagggc aaaaaaggca agaagaaaag
     2821 cgtcgaactg gtgctggacg aagaacgcgg cgaagtgatt ggccgtaaaa agcacaagcg
     2881 cggcgatctc gattttggcg atgatgagta acatctcaca cctatcgctt ctcactggcg
     2941 agaagtgaaa ttaaggagca tgtcgaagaa acctgttcag cgtgtaaaac atgtacccca
     3001 gcgtacctgt gtgggctgtc gtgaagttct gcccaaacga aagatgattc gcattgtgcg
     3061 aacaactgat ggggtgcgag tggacccaac cggcaaactg gctggtcgag gggcttacct
     3121 gcatgaccgt cgcgattgct gggcgcgtgg tttgaaaggt tctttagcgc acgcgttgaa
     3181 aacgactttg acatctgatg aacgtgccag actggaagat tttatgaacg ctttgcccgc
     3241 tgatgcggag gcagagttag cagggggcgg gtaagtaacc atgtgacgcg tagcgtgtaa
     3301 attgctcact gggcttaccc aatgaacaat gtgttcgtgt gttcatttga agttgtgaaa
     3361 caaggaggca gcccatgagt attaatggaa ataagattga attgccgacc agtattgtga
     3421 ttcgcgatct ggcacaaaag cttgaaaaaa gcccaattga cctcatcaaa aaactgatgt
     3481 ccaacggtgt gatggcaacc atcaaccagt ccgtggattt tgatacggcc gcgattgtga
     3541 tggctgaata tggctatgaa gcagttcctg aagttgtaga ggaagttaaa gttgaagttg
     3601 gggaagttcc actctggcgt caattgatcg ccggggaaga tcaattattg ttgaagaacc
     3661 gtccgccggt cgtgacaatt ttaggtcatg tagatcacgg caagaccagc ctgttagatg
     3721 ccatccgcca aacggaagta gccgctgggg aggctggagg tattacgcaa cacatcggtg
     3781 cctatcaggt ggagatgaag ggacggcaga ttacatttct ggatacccct ggtcatgcgg
     3841 cgttcacaca gatgcgcgcc cgcggtgcac agggtgcgga catcgtcatt ctggttgtcg
     3901 ccgcagacga cggcgtgatg ccacagacca gagaggccat tgcgcatgcc aaggcagcgc
     3961 gtgtgccgat catggtcgct ctgaataaga ttgataaagc caatgccaat ccggaccgtg
     4021 tgaagcagca gcttgccgag ttggaactcg tcccagatga gtggggtggg aacaccatga
     4081 tcatcccagt ttcagccaag ttaaaacagg gcatcgatga tctcttggaa ggcattctgc
     4141 tggttgcgga taacattgaa atcaaagcaa atcccggcgg gaaggtaatt ggcacggtca
     4201 tcgaagccga ggtggataag accaagggcg tgatggcaac tctgctggtt caaaatggaa
     4261 cgcttgagtt tggcgatatt gtcgtcgctg gaatagctca cggcaaatta cgtgctcttt
     4321 ctgattacag aggcaaaccg gttaaaaagg ccagcccttc tacccctgtc gcagtgatgg
     4381 gattgagcga tgtgccctct gcaggcgatt tgttcgaagt ggtcgattct gagagggaag
     4441 cacgcagcat tgttgcagaa cgtgcagaag ccgcaaaatc gcaggcacag gcgaagaaaa
     4501 aggtatccct tgaagacctg tttgcaagcg tgcaggccgg tgaagccaag gaacttaacc
     4561 tgattgtgaa ggcggatgta caaggttcac tcgacccaat cgtttccgag ttgaacaagc
     4621 tcggcgaagg ccaaatcgga atcaaaatcc tctattctga aacagggaac atcggtgaaa
     4681 acgatgtaat gttagcatcc gcatcgaacg cgattatcat cggtttcaac gtccaggcag
     4741 atgtctctgc tcgccgcctt gctgagaaag agggtgttga tatccgctta tatgagatca
     4801 tttatcgtat gacggaggat atcgaaaaag cactcaatgg tatgcttgag ccggaagtta
     4861 aagagaagat catcggccgc gcgcaagtgt tgcaggtttt ctcggcgtcc aaatttggta
     4921 aagtagcggg atgtagagtg accgatggtg aattacgacg caacgctaaa gttcgcctct
     4981 tccgcggcac cgatttggtc tttgaaggtg atatggggtc gctgcgtcat gagaaggacg
     5041 acgtgcgaga agtcaaacaa ggctatgagt gcggcgttgg tttcaaaaat ttcagcgata
     5101 tccagtctgg tgatcaactg atttgttata tacttgaaac gtcataggaa gacagttaat
     5161 cgtaaactaa acgctcatgc cttctggtac ccgtcttcaa cgtattgcgg atcggatccg
     5221 ccaggaactc tcggagatgt tgatccgtga gatcagtgac ccgcgcttga agcaaattta
     5281 catcacagat gtgaaagtgg ataaggaact tgcctacgcc gatattttcg tttcggccat
     5341 agagggagtt tcacgctcca aagacattct cgcggggctg gagtctgctt cgggttacgt
     5401 ccggcggact cttgcctcgc gcgtcgagct gcgcgctttc ccgcgtctgc gctttcattg
     5461 ggatatgacg ccagaaaact cagatcatat cgagaaaatt ctggccgaat tacgagcgaa
     5521 gaaatagccc cagtatctga ctggtaataa gaaattgaga ggatattcaa ttaaaatgga
     5581 tcaagcgtca aatgcaatca aggcgcgtct ggaacaggcc acgaacatca tcattgcatc
     5641 acacgtccgc cctgatggag atgcaatcgg ctccctgctg ggcttagggc tggcgttaca
     5701 gaacgcagga aaatctgtgc aaatggtact ggtagacggg gtgcccgctt cctacagaca
     5761 cctggaagga agtgaaacaa ttgtcaagca gccaggtgga gaacacgata cttttatcac
     5821 tgtagattct gcggatttca gaagagtggg aaaggtgttt gaaaatttcg gtcagcctga
     5881 tatcaatatc gatcatcaca aaacaaatga aaaatttggc aaactgaacc tgatcgaagc
     5941 cgaagaagtt gccactgcag ccattctcac caatcatttg cgcgaatggg gatatgaaat
     6001 tacaaaaccg attgctgacg cattattgac aggcatcatt acagatacgc ttggttttcg
     6061 taccccgtcc actaacccct cggcattacg tttatgtgcg acgctcatgg aaactggtgc
     6121 tgatatgcca gatctctaca tgaaatctct cgtcaggaaa tccttcccgg cggcgcgcta
     6181 ctggggggca gggctgagca gcatggaaga gaagaatgga attgtgtggg gaacgctgac
     6241 cgtggaagat cgtaaaaagg cgggttatgg tggaaacgat gacgctgatt tgattaatat
     6301 gatctcagct atcgacggta acaaagtagg catgattttc gttgaacaaa cggataacca
     6361 tgtcaaaatc tcctggcgcg ccttggaacc aggtatcgac gtctcaccga ttgccaaaca
     6421 ttttgggggt ggcggacacg ccgcagcagc gggggctgat attcccggaa cgttgcaaga
     6481 catccaacca ttggtgctga aaacaacgta tgaaatgctt ggattcgaaa gatagaaaat
     6541 aagatagtat aatttaattg aaatgggagt gagtcttaag gaggagtatg acaatgaata
     6601 gtcaggacgt aaaaaatgca atttcgggcg ttttggtcgt agataagccc gtaggtatga
     6661 cgtcgcacga tgtggtgcag gcgatccgta atggaacagg tctgcgccgt gcgggtcaca
     6721 cgggaacatt agaccctcgt gcgtcaggtg tattggtaat tttagtaggc ccggccgtac
     6781 gtttgagtga gtacgtttca gcttcagata aacgatatca agccattatc cgtctggggg
     6841 gaagaacaga tacctttgat gccgatggaa aggtcacaca ggcgacccag cctgtgaacg
     6901 tgaccgaatc ccaattcgaa gaagtattaa aaacctttgt tggtgagatc gagcaaactc
     6961 caccgcctta ttccgctgtt aaagtgcagg gacgcaaagc ctatgaaatg gcacgcaagg
     7021 gcgaagaagt tgaactcgaa ccacgcaaga tcaccgtcca tcatcttgaa gtgctcgaat
     7081 gggcaccgcc tgaggtcgtg attgacgtac actgttcgtc cggtacgtat gttcgttctc
     7141 tggcaaatga tttgggtgaa aaacttggct gtggtgctta cctcgttggc ttgcgccgca
     7201 ccaaaagtgg aagattttct ctgcgcgatg cagtccctct acgcaaatta caggaatcgt
     7261 tccatgcggg taactggtat caatatctta tcccggcggc ggaagcactg ggtgattggc
     7321 ctgccttgga acttagtccc gatgatgtag aaggtgttcg gcatggtcat cgggttacgg
     7381 cagctgcaga tgcggtcatc ggaagccgtg tccgaggtgt aagtacgcag ggtgaactgg
     7441 tcgccttgat ggaatgtgtg actggcgaag atggtagccc cgcctggcag ccgaagaaag
     7501 tattttttac ttcagaatag atgacatgaa cacgaccgtc agtgtactct ctggcggcta
     7561 agccaccgat ggaaggtcgg tgatattcca gcgccagcga cggcgctgga gttatttatg
     7621 gtatgaaaca ttatcattct ctcgaagaag tttctttaga aaattcctgg ttaaccgtgg
     7681 gtgtctttga tggagttcat cgcggtcacc aggaaattat ccagaaatta accaacgacg
     7741 ctcatgccaa tggcaatcct gcagtggttt tgacgttcca cccacatcct gccaatgttt
     7801 tagggcgcgg cgagatcggt ttgttgacgt tccctgctga acgcgcggaa ttgctggcat
     7861 ccatggatgt ggatgtggtt attactgaac atttcaccag ggaactttcg tcggttaccg
     7921 ctttcgattt tatgtcacgc ctgaagcgta accttgggct aaagcagcta ttaataggct
     7981 atgattttgc actgggcaaa ggacgtgaag gcaatgcgcc gcgattaacg gaaatcggcg
     8041 gtgagcttgg atacacagtc gaagtggtat ctgctttgag cgatgagagc ggtgtgattt
     8101 catccaccga aatccggaaa ttagtttcag ttgggaatgt cgccgaagct tcgcatttaa
     8161 tgggtcacgc atattctcta ggcggtcccg tggtacgcgg cgacgggcga ggaaaaggga
     8221 ttggcgtacc cacggcgaat attgaatatc cgcgtgaaaa aattgttccg gcgaaaggca
     8281 tttatgcagg ttgggcgcat ctcgatggtg aaagacataa ggctgcgatc agcatcggag
     8341 taaacccgac ttttactcca gaaaaacaga ttcccagtgt ggaagcctat ctccttgatt
     8401 ttgaccggga catttatggc aaagacctgt acatcgagtt tgtcgcacgt ttgcgcgacg
     8461 agctgaagtt cgactcggtg gacgcgctgg tggaacaaat ttggaaggat gtggaagagg
     8521 cgcgcagtat tcttggttca taatgtcaga aaaattgcag ttgctctttt ccgattgact
     8581 tcaaattcca tttattgtac agtgtctatt agttcaactc acaagggaga tgaataatga
     8641 cacaatcttt caatcaaaat cctgagccat tgcaaccgct ctcagcagag gggtctccac
     8701 cattcaatcg agaggcagaa attgccgaga tcacgcaacg gatgaagcgg ggcgccaata
     8761 atttttactg gatcgcagct ttatctgtaa tcaattccat tctttcgatg accgggagca
     8821 gtacttattt tgtcattggt ctggcgatta ctctggtcac agatgggata acgataggtc
     8881 taagccagtc tcttcctgat gcggctgtga tcgtaaagat cgtggggctc gtgttgagtg
     8941 tgctgatcgc aggcgtcttt gccctctttg gatattttgc atcccaggga aagcgttggg
     9001 catttctggt tgggatggtg ttatatgctc tggatggact gattatgctt gcgtttgctg
     9061 attggattgg cgttctgttt catgcttact ttctttgggg gttgtttggg ggatggcgcg
     9121 cacttggtga attacagcaa aagtcgtctc aaaaccagat ggcttttcca caagatatcg
     9181 gagttcaatg accttcgggc ggcggtgagc cgcccttttc attgatataa tctgccagat
     9241 tttatagagg agacaactaa tgcctgaacg tgaaatttac cttctttcgc cgcgtgccct
     9301 tagcccggag accatcgcgg ttgcctttgc aaagacctcg cgctcccctg aatcttttcg
     9361 tgatattgcg gcagaattaa gtgatgagaa atccgcacaa ttccatgaaa agtgggtggt
     9421 gggctatggt catgcgtcgg tggccgagca tgcagtgtta catattgcct ttgagaatgt
     9481 atcgcggctg gcgattgaaa gcatcgaagg aaatcgtctt gcatcgtata ccgaaaagtc
     9541 cacacgctat cagaagtggg gatcagatga ctttaccgtt ccgcctgagc ttacggatca
     9601 tcagcttcgc gacgagtttg aagatacagt ccgtctcctt ttcaggacgt acgctgagtc
     9661 gctggaccct gtgcggaatc tcatccttga acgtttcccg cgtcgcgaaa atgagaagga
     9721 tgaagcctgg gatcgccgca tccgttcgaa gtatgtggac gtctgccgtt ttctcctgcc
     9781 tgcggctgca cttgccaacg tcggcatgac tgccaacgcg cgagtgttgg agaatatgat
     9841 ccgcaaaatg ctctcccacg aactggcgga ggtgcgcgag atcggtgcga gggtcaagga
     9901 agtggcgaag agcgaagccc caacccttgt caagtatgcg gatgctgtgc cttatttgat
     9961 ggaaacaatg aacgaattta gccaccgaga cgcggagtcg cagagcataa aaaaatctcc
    10021 acgtctgagc gactctgtgg cagatcagtg gtgtacatta gtcgattatg atcaagatgg
    10081 ggaggagaat gtgtttgccg cagcactcta tcgctttggc gagatgggtt atgcagatgc
    10141 actgtcctat atcaaaaccc ttggtgagcc ggagcgtgaa aaattagccg aaatcctcct
    10201 ggcgcgtctg ggaaaattcg atgtgcccct gcgcgaattg gaatattgta cctatacatt
    10261 tgacctggtt atggaccagg gcgcgtatgc agagttcaaa cgtcatcgca tgatgacgca
    10321 gacgccgcag aaactgacga cgaggttggg atatgcgacg ccgcgtcttg tcaccgaggc
    10381 gggttttgag tctcaatatg aagccgcgat ggaagccgct tcacaaatgt tcgaaacgtt
    10441 ataccaattc aatccgaatg tcgcgcaata cattgtcccc aatggattta atcgccgcgt
    10501 gctggctcag ttcaacctgc gagaagcgtt tgctttttgt cagttacgtt ccgcagccaa
    10561 cgctcatttc tcaattcgga gagtggccca acgcgtatac gaagaaatgg cgcgcgtgca
    10621 tccgctgctg accaggtata tgaagctgca tgatgaaaca tggcaagggg ttgaggaact
    10681 tcatttcaca ggtatgtaaa aaaaactccg agtcgtagac tcggagtttt ttgctgcatt
    10741 tttacagtct ggttattaaa acccgttttt ctttattaca ttacgatacc acttcgcgct
    10801 atcttttagg atacgcttct gggtctcgta atccacccac acgagcccga aacgctgggc
    10861 ataacccctt gcccattcga agttatccat gagagaccag gcgaagtaac cggccattgg
    10921 cacacctgcc tggatggcgc gatgcaccgc cgcaatatgc gtccgatagt aatcaatgcg
    10981 gagatcatct ttcacgcggc cagtttcatc tggcggagtg gagtagctgg cgccattttc
    11041 agtcacgtac attttcaatg gcttgtaatc aaaatacaaa cgcgagagta caccggtcag
    11101 gccttcgggg aaattttccc agcccatctc cgtccaattt tcaggtgttt ttggtttgga
    11161 aaataccacc tgtgtttcat tatccgacgc caccgagcga accacctcac ggttgtaata
    11221 gttcactccg aggaaatcca gtgggacaga gatggatttc atatctccat ttttaatgaa
    11281 actcatctga tttggcatgg catcctgtcg ttcaaaatct gcgactacat cggcgggata
    11341 accacgaccg tagagcggat cagtaaacca gcgtgtccat ttgccgtctc cctctcgaac
    11401 cgcgttcaag tccgcagagg agttggatgc tgggactctc cacccgacgt tgagcgtaat
    11461 gcccacttcg gagtcgggac tgttgcgtcg aagcaccggc accgcccatc catgcgagag
    11521 aagcagatga tggctgacag ccacaccctt ggccagatct ttgaagccgg gtgcatgttc
    11581 accggtgtca tttccgatcc aggaaacgac cgcgggttcg ttgtgcgtga tccaattctt
    11641 aacgcggtca cccaacgctt ttgagatggc ttcggtatat tccacataag cttcagccgt
    11701 ggatctcact tcccatccgc cttcatcctg caacacctgg ggtaaatccc aatgatagag
    11761 ggtgacgaag ggaatgatat ttgcttcgag aaggccatca acaagtttgc tataaaaatc
    11821 aattcctttt tggttgacct tgccgcgtcc tttgggcagg atgcgcggcc aggcagtgga
    11881 aaatcgatag gctttcaatc ccagttgctt catgatgcgg atgtctttct tccacaaacg
    11941 atagtggtca ttcgcaacat cgccggtatc accattgtga atcttgtctg gtgtatggga
    12001 aaaacgatcc cagatggatt cacctttgcc gtgtttgtcc caactgcctt caatttggta
    12061 ggaagcggtt gccgcgcccc agatgaaatt ctcagggaat atgatttttg tcgccatgtt
    12121 tggattcctt tgtagttcga tttgtattta tgatgttttt tatgttttta ctatgggcat
    12181 tctaaaattt cagggttagg ttcttggtat gttagaccat aaccgaatgg aaacaacggg
    12241 gcatcacagc cagtcttatc cgctgaatta ttgatgttga tgggtagctg ctcgttcgag
    12301 cgcggccatg agtaggatag ttttcctgta aatggaaagt ctccgaacaa cacatctgca
    12361 attccaccgc cttctgtgcc gggaagccac gctgccaccc acgcctccgc taaaggtaat
    12421 tgctctgtga tgacgcgtgg tcgcccagaa agcaaaatta caaccagatt tttactctgc
    12481 tccctggctt ccatgatcaa ctgaatttca gcatcattca gcgaaatatc tgcctgatcg
    12541 ccgacacctt ctgcatacgg acgctcagca agcacaacga tcccgacatc cgcaatgagc
    12601 tgatttccat ctccatcttt gaattcactg aagtccgcat cgcggttgaa caagacctgc
    12661 gcgtcagaat ccgccactgc tctgatcccg ctgaaaattg tggtgccttc gttgtcatcg
    12721 ccaggcttgc cctgccattc gagagtccag ccacctgatt ggagcccaat atcattggcg
    12781 gcttcaccgg caacaaagat gctcggtgtt tccttgctca agggcaaaac gttgttatcg
    12841 ttttttagca ggactaacga ttccctcacc gcctgacgtg caagttcgag atgctcacgt
    12901 gaacggattg tcccctgata cttcgtgtca ggcattggct gttcaaataa tcctaaggca
    12961 aatttcacac gtagaatacg gcggactgca tcatcgatgc gtgactcggg gatgtcacca
    13021 ttcgttacgg ctttttgcat ggtttcgata aacgcgacgt actcatacgg taccatattc
    13081 atatccacac ccgcgtttat ggaagtgacg acagagctat agtagtcatc cgggtaaatc
    13141 tgattaatgg cctgccaatc cgaaacgatg aacccattga agccaagctc ttccttgaga
    13201 atatctgtga gtaagggctg ttgggcatga agtttgattc ccttccagct actgaatgag
    13261 gccatcacgt tcattgcccc ggcttctacc gccgcctgat agggaggtag aaataactcc
    13321 cgcaggatag tttcagggac ctgcatattg ccctgatcaa gtctgtagtc ctccgtgcgc
    13381 gatgaatccc atatggtggc gccatcccca ataaagtgct tcggcgttgc gagagcgaac
    13441 agagattggc ctgcggatgc ctcatcgttg tctttcaact gttgcgtgcc acggatgtat
    13501 gccgtaccaa gcgacgagac aatttctgtg ttttcgctaa agccttcata cgtgcgtccc
    13561 cagcgcacat cctgtacgac ggctatcact ggcgcaaaat tccacgggat gccgctggca
    13621 agcatttctt cagcagtggc gcgaccaatt ttttctacaa gttcagggtt gtttgctgca
    13681 cccagaccta tattatgtgg aaaaaccgtt gcgttgagca gattgccatg accatgcact
    13741 gcatccacac catagatgag gggtatctcc aggcgtgtcg caagcgcctc atcttgaaac
    13801 gcttcaacca tggcatacca gccctgcgcc gtattttctt ttggcgcgcc gccaccacca
    13861 cttagaatcg acccaatata atatctggtg atgtcaccgg ctcggatgct attcttttcc
    13921 acctgagtca tctgcccgat cttttcttcc atagtcatac gttggagcag gtcttcaaca
    13981 cgtgcttcca cgggttgtga ggagtctgtg tagataacag gtccaaactc atcatttgca
    14041 attgtggttg gttcggtaac aggggcaggt gttactgatt caccggtaca ggaagcgacc
    14101 gtcagagaaa tcaggaaaag gaaataaaaa atgcgtttca tggcatttgt tgacatgcgc
    14161 ttcgcctatc ctcttcccat gtgattttca attacaccat ctttcaacgt caagacctga
    14221 tgaacatatt gatccaccag cggatcgtgt gaaaccatca gcatcgtgat atgctgtgtc
    14281 tcaacaagtt cccggaagaa tgtaagcagt tcacgcgttg tcttgctgtc caattcaccg
    14341 gtgggttcat ccgccagaat caattttgca tgattcgcca gggcccgcgc gattgccacg
    14401 cgttgttgct gtccgccaga catctcgtat ggacggtgat cgcgccattt acccaaccca
    14461 accaaatcca ggcattgcaa cgcacgctgg tgacgtgcct catattcatc gcctttgatg
    14521 cgcaggagta actcaatatt ctcataggcc gaaagcgtgg gcaacaaacc aaacgattga
    14581 aataccagcc cgatttgttc gcgtcgccag cgagttaact catcatcgct catttgcatc
    14641 aaatcattgc caagcacaat cgccgtgcca gaggttggtt tatccagtcc agccaaacaa
    14701 ttcaacaggg ttgtctttcc gctgcctgag cgtcctttga gcgttacgaa cgaacccgac
    14761 tccacattta gattcacgcc gcgtaaggcc tggatgcctt cctcgttcct ttttgaagga
    14821 tagacgcgcc gcaggtcttt tgtttcaata accagactca ttctttctcg cctcgtgcaa
    14881 atctggattt cacactttgc catatcctcg accaacctga aggaagttgc acgtcttccg
    14941 actcaggtag ttcctcctca ccggcagaaa agctttgagt gtggccgtga tgattccctt
    15001 ccggtgggcg gatcaaaatc ccctcttctg tcatttctat ctgcacccga cgattgatct
    15061 ggaactgctc caggtactcg cgcgggattt gcaggcgacc tgcagaatcc aacacggcca
    15121 gttccaccag atgatgctcg tcactatctg atttttgaac gcgcagggtt tcactcgcca
    15181 gtttgccatc gcgcaccgcc accacccggt ccacatgttg ggcgataccc ggatcatgac
    15241 tcacaatgac gatggttaga cccaggtcat tgtttaaagc acgaaacaaa tcgtaaatgc
    15301 tttttgccgt tgcggtatcc aattcacctg taggttcatc ggctagtaag agtttgggac
    15361 gatttgccag tgcgatggca atggccacac gttgctgctc accaccagac aaagcttcga
    15421 ggtgatgatc tgcccgctcg ctcaaaccaa cgatcttcag caaatcagtc gcgcgcacac
    15481 ggctggaatt gccgacctgt cctgagagtg tgagcggcag ttcaacgttt tccagcgccg
    15541 tcaaatatgg gatcaaattg cgcgtggctt gctgccatac gaaaccgact tgttccaggc
    15601 gataggcatc cagggcacgt tcactgaaac tgcccaaatc ctgtcgttcg acgagcacgc
    15661 gcccgccagt ggggcggtcg agaccaccga gaatatttag tagggtggat tttccactcc
    15721 cac
//
LOCUS       DASTJP010000292         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1104710, whole genome shotgun sequence.
ACCESSION   DASTJP010000292 DASTJP010000000
VERSION     DASTJP010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104710"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..996)
                     /locus_tag="VFQ23_08840"
     CDS             complement(97..996)
                     /locus_tag="VFQ23_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9906736.1"
                     /translation="MSACAPQPTVSTAQPITLVPYLTSTQSPSQTPQGLVTAETPLPT
                     PTPFTYTVQSGDTISGIALKFGVSIDDLQAANPETPPNAMSVGTVLNIPSNPENPSGE
                     PTPTPASFSIRQIECYPTSNKGMWCFVLVHNDFSDFMENLSAQVTLVDSNNVVLASQT
                     ALLPLNILPPNTSLPLSVFFSPAVASDAMPQVQVLTAIRLLPNDQRYLPATIHNTFIQ
                     ISADGHSARVSGQVLSQSASIAATQVWVAATAYDETGRVVGVRRWESSAVLSAMGSLP
                     FEFMVSSIGGRITRAEFAVEARP"
     gene            complement(1073..1342)
                     /locus_tag="VFQ23_08845"
     CDS             complement(1073..1342)
                     /locus_tag="VFQ23_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9906737.1"
                     /translation="MPVVGIPSLLRDLTNGQRLLSVEGDTVRQVIENLETLHPGIKER
                     LYDGDRLRPSIAIVVDGHTSTLKLRHRLKESSEVHFVITISGGAF"
     gene            complement(1347..>2327)
                     /locus_tag="VFQ23_08850"
     CDS             complement(1347..>2327)
                     /locus_tag="VFQ23_08850"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9906738.1"
                     /translation="LETLEAAQNSSHYRAPLDGPNRGRGVASGFWFNYGGKSSASASI
                     NSDGTISLITGSVDIGGTRTSIAMQLAEGLGLPVESIRPGVADTDSVGFTEGTYGSRT
                     TFATGWAVYEISNQMKEELLKRAAIIWETEPDTIRFAHGAFLFEDQQMTFAELASRLD
                     DTGGPVVASAAVRPEQSGPAFATHIVDVEVDPETGKVQILRYTAVQDAGKAIYPNYVE
                     SQIQGGVAQGVGWALNEEYLYDDQGRLTNSSLLDYRMPVTLDLPMIDAIIVEAPNPGH
                     PYGVRGVGEVPIVPPPAAVVNAIYHAIGVRMNVLPATPARIVAELNKQEG"
BASE COUNT          585 a          597 c          590 g          555 t
ORIGIN      
        1 ggatatgtcc ttacgccgat tgatataccg attgagcgat atatgtggtt ggacgggaag
       61 tagccctcac ccagccttcc cgaagggcgg agggtgtcag ggtcttgctt ccaccgcaaa
      121 ctcggctcgc gtgattcttc ctccgatact tgataccata aactcaaaag gaagacttcc
      181 catcgcagat aaaactgcgc tggattccca gcggcggact ccaactacgc gccccgtttc
      241 atcgtacgct gtggcagcca cccaaacctg agttgcagct attgaagcgc tttgtgataa
      301 gacctgtccg ctgacccgcg cactgtggcc atcggcactg atttggatga aggtattgtg
      361 gattgttgcc ggcaggtatc tttgatcatt tggcaataaa cgaatcgcgg tcagcacttg
      421 aacttgtggc attgcatcgg atgcaaccgc aggtgaaaag aaaacagaaa gcggcaggga
      481 cgtattgggg ggaagaatat ttagaggcaa taacgcggtt tgactagcta atactacatt
      541 attcgagtcg accaacgtca cctgcgcgct caggttttcc ataaagtcag aaaagtcatt
      601 atgaaccagc acaaagcacc acataccctt attggatgtt ggataacatt cgatttgccg
      661 aattgaaaaa gatgcaggcg taggcgttgg ctccccagat ggattctcag gattgctggg
      721 aatgttcaac acggttccga ctgacatggc atttggcggc gtttcagggt tcgctgcctg
      781 caaatcatca atagagactc caaacttgag agcaattccg ctaattgtgt ctcctgactg
      841 tactgtgtag gtgaaaggtg ttggtgtagg gagaggtgtc tcagccgtga caagtccctg
      901 gggagtctga cttgggcttt gagttgaggt gagatatggg acaagcgtaa tcggttgagc
      961 agtagagacc gtcggttgtg gtgcacaagc ggacaaaaag atgatgaatg ataaaaaatg
     1021 tatgacgaaa taattggagc gacacatcag acgatgattc gagtttggct cattaaaatg
     1081 ccccgccgct gattgtgatc acaaaatgga cttcgctgga ttccttgagt cgatgacgaa
     1141 gcttcaatgt gctcgtatgc ccatccacaa caatagcaat ggatggacgc aggcgatctc
     1201 catcatacaa acgctcttta ataccgggat gcagcgtttc cagattttcg atcacctgcc
     1261 gaaccgtatc cccttccaca gagagtaacc tctgtccgtt tgtaagatcg cgtaataatg
     1321 agggaatccc aacgactggc aaaaaattat ccctcttgct tattcagttc cgcgacaatt
     1381 cgcgcgggtg tcgcaggcaa aacattcatg cgcacgccga tagcatgata aatggcattc
     1441 accaccgccg caggcggcgg gacaatgggc acctctccca cgcctcgcac gccatacgga
     1501 tggcccgggt tgggtgcttc aacaatgatc gcatcgatca tgggcaggtc cagagtgaca
     1561 ggcatacggt aatccaacag gctcgaattg gtcaggcgac cctgatcgtc atagagatat
     1621 tcttcattca aagcccatcc taccccctgg gccactccgc cctgaatttg actctcaaca
     1681 tagtttgggt aaattgcctt cccggcatcc tggacggctg tataacgcag gatttgcact
     1741 ttgcccgttt ctggatccac ttcgacatca acgatgtgag tcgcaaatgc gggtcctgat
     1801 tgctctggtc gaacggcggc actggcaact acaggaccac cggtatcgtc caaacgtgag
     1861 gcgagttctg caaaggtcat ctgttgatct tcaaacaaaa aagcaccatg cgcaaaacgg
     1921 atcgtatctg gttcggtttc ccaaataatt gcggcacgtt ttaatagttc ttctttcatt
     1981 tgattgctga tttcatagac tgcccaaccg gtagcaaacg tcgtgcggct tccataggta
     2041 ccttcagtga atcccaccga atctgtatct gcgactccgg ggcgaatcga ttcaaccgga
     2101 agccccagtc cctccgccag ttgcatcgca atcgaggtac gcgtaccccc aatatccaca
     2161 gaacccgtta tgaggctgat tgtgccatca ctgttgatac tcgccgacgc gctggacttc
     2221 ccgccatagt taaaccaaaa gccggatgca acgccacgcc cgcgattcgg accatccagc
     2281 ggcgcgcgat aatgtgatga attctgggca gcttcgagcg tctctag
//
LOCUS       DASTJP010000293         9978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_589877, whole genome shotgun sequence.
ACCESSION   DASTJP010000293 DASTJP010000000
VERSION     DASTJP010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589877"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFQ23_08855"
     CDS             complement(<1..733)
                     /locus_tag="VFQ23_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9906739.1"
                     /translation="MESISVLVVDDEPGIALLCNRILSRADYQVTSLTNPREAIEFLQ
                     KNHVDLLLVDIRMPEVDGFDVISRAQMVLPDIAVLVMTGFGTVETAIRALRQGVDGLI
                     LKPFKKSDELIQAAKQALTDNQRKRDTARVQALRPLFNVTETLFAETDQSKLLNMIIA
                     AISTHLHCSNVAYYQIENGSVSVVAQRGKALPVDDSDFAAQLVSRVDADGDPIVINAT
                     GPGEADTQSLLSTLGIGSAILIPVVR"
     gene            complement(804..3455)
                     /locus_tag="VFQ23_08860"
     CDS             complement(804..3455)
                     /locus_tag="VFQ23_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9906740.1"
                     /translation="MPHQMTIEAQLKEASQLTNATWAALVEREAGKWRIISHFHLAKK
                     VQPALIKFLSKAEVDSWLCGALSGGQSRSVSVSNLSTLEAARLFAFPLPGVSRAVLAG
                     ADQLTNESQRLWRLVVAGMQTEDMPADASALSSVAASLLIPGLNSESPYDLDRSLDRA
                     LTSFVRLVSVQGALLAVLRGDFLDVRAQWNAPSLADLELSIDNHTLLRRMASNLTPIV
                     VHRGDESWSQIPHKGLKSATRLWACVPLVIGQRLIGTLILWRTSDFKGDEWNRMVDFA
                     TQVAPAIETLITFDEMTGHLLRLGMLNDFAITVSSGRNLDQIARRLFALLARAFRTEL
                     IVLFLLSSDNRLLNEYRTVDGGTLVPGSGAGVTSTVRSAEEHKITPLLANGQKVRLSD
                     SQTDDNLPLYDKALSGLYVPLRFRGQTIGMLCVESLRTNAFNLYDESLMTVITSHLAS
                     LADYTRLREEAEGRARNLGLIHEVVQQVIGLTDQREVAIITSDLLARYFAYELAAVFI
                     ADEKGNLTIGGFGGTSQNVVKRAMKSFEYPVMGGITGYVFGTGESIMVNDVLHDKRYR
                     SIKGWQAGSEMCVAIREGERILGIIDVESSSRNAFTYNDFMALESLAGILASVITSAD
                     QYQRLQVTISQLRSTQMELRARMEAQRSAENRLIQAAKLAAVGEMAAGIAHELNNPLT
                     SVTGFAELVLEEMPQESESRKDLETVMREAIRARDVVRRLLDFARQSESTRAKASLNE
                     VVEDVVALSKHLIHISGIELKIDLEKNLPWVLVDANQMKQVLINLVHNALQAMPDGGE
                     MEIRTSNASRDGRDWVTVSVQDSGVGIPQLDQARIFEPFYTTKGNQGGTGLGLSVTYG
                     IITDHGGQIEVLSQPGAGAKFTVWLPL"
     gene            3520..4902
                     /gene="trmFO"
                     /locus_tag="VFQ23_08865"
     CDS             3520..4902
                     /gene="trmFO"
                     /locus_tag="VFQ23_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET9906741.1"
                     /translation="MTDLIVIGGGLAGSEAAYQAAQRGLKVQLFEMRPNLQTGAHQTQ
                     DLAELVCSNSLGSNLSDRASGVLKNELRMLGSMLLECAESASLPAGGALAVDRELFAQ
                     LVTQRIEAHPNIEIVREEAKEIPDSPSIIASGPLTSTALSAAIAELSGEKHLFFFDAI
                     APIVQAESINMEVAFRASRYEKGDLEEGDYINCPFTKEEYYTFVNTLLEAQRIELRSF
                     EDAIKSGVKAGHFFEGCLPVEIIAERGVDSLAFGPMRPVGLRDPRTGKRPYAVVQLRQ
                     DNLAGSLYNLVGFQTNLKFPEQKRVFQLIPGLERAEFLRYGQMHRNTFIASPKLLRPT
                     LQHLNRDDLFFAGQITGVEGYMGNIATGLLAGWNAARLLHNEEPIVLPQTTMLGALCH
                     YVTHADLKDFQPMKANFGILPPLEFTSKIGKRERGSAYAERALTHLQQMLSEERADDR
                     EQVLKPAGND"
     gene            4895..5803
                     /locus_tag="VFQ23_08870"
     CDS             4895..5803
                     /locus_tag="VFQ23_08870"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9906742.1"
                     /translation="MTKKFALLYLAIIGLLLTGAIGWYVFAFRSEPKADTSVFNGQRA
                     YEDVKTQVALGPRWPGSEGHAQLQEWIRSELEAAGWQVEIQQSEAMGHPIENIVAKKS
                     ETSPQIIFGAHYDTRIYADHDPDSSKHEEYVPGANDGASGVAVLLELARTLPEDTPPI
                     WLVFFDAEDNGNIEGWDWILGSREFVKNNTLDPQAVVIVDMIGDADLNIYKEYNSNPE
                     LTNEIWDVAKSLGYEDKFIPDHKHSMTDDHTPFLEAGMPAIDIIDFDYPYWHTTQDTV
                     DKVSGESLEIVGKTLWTWATQQTPQP"
     gene            5860..7101
                     /locus_tag="VFQ23_08875"
     CDS             5860..7101
                     /locus_tag="VFQ23_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9906743.1"
                     /translation="MPTKNSSTPIQKALAKIRPAWIARVGQDLARGMEVRAGFEEQLK
                     RFFELLVQSVTTGDPAWMDPILLDWAKSSTETDLEEGQYHVSFLMNRMIALTIQVSRE
                     SLTKQQALDLLASVIPIYTYGLEVVARYEMETRVAHISSEMEKVQQRMEKVDKSKSAF
                     ISVAAHELKTPITLIEGYASMMDDLAQDKNNGTLGSLLTGMNTGIGRLRTIVDDMIDV
                     SLIDNQLLTLNFQPIQIAHTISAIQMEVASILANRKLSLEVLEFDGSKQWIYADSTRL
                     MQALQNVISNAIKYTPDGGKIKIDGRTLPGFIEVIVTDTGIGISAENQSMIFEKFGQL
                     GRVELHSSGKTKFKGGGPGLGLPIARGILEAHGGSIWVESEGYDEIRCPGSTFHILVP
                     ARTDTPDVEMKKLFDTLDKRK"
     gene            7108..8469
                     /gene="hflX"
                     /locus_tag="VFQ23_08880"
     CDS             7108..8469
                     /gene="hflX"
                     /locus_tag="VFQ23_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9906744.1"
                     /translation="MAKRIPQPTVAPRERAFLVGVDLYQQKNFLSLGDSLTELALLAD
                     TSGLDVVGELTQKLDRPHVKTYIGPGKVEELKALVEETLTQVIIFDDELSPRHQRELQ
                     EIMGRNVRVLDRTALILDIFAQHAHTSEGMLQVELAQYEYYLPRLTGQWTHLERQAGG
                     GGGRAGSTGGVGLRGPGETQLEVDKRAIRKRISHLKKELEKVRAHRMRYRAQRKRSRI
                     PTVALVGYTNAGKSTLLNKLAKSDVFVADQLFATLDPTTRRVELPGGYQALITDTVGF
                     IQKLPTTLIESFHATLEEIVEADLLLHVVDISHPNALNHFESVQQTLEELGANHIPVV
                     TALNKMDNLRDPEAAKEAVRRFSKSVTISGLKGTGISELLKLIQEALYETYAPILVRL
                     PYQQGALISLFHEAGQVERIEHGRGGVLMQGRIPGRLLAQFVQWQVSSNYREPELEEE
                     EEL"
     gene            8466..8678
                     /locus_tag="VFQ23_08885"
     CDS             8466..8678
                     /locus_tag="VFQ23_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906745.1"
                     /translation="MKWLSRLVDRASDYFAHRKGLLPLIGILLVIINYVLPFIFGLDN
                     LITGSNLFLHLGVVVAIFGMMLASAL"
     gene            complement(8712..9533)
                     /gene="murI"
                     /locus_tag="VFQ23_08890"
     CDS             complement(8712..9533)
                     /gene="murI"
                     /locus_tag="VFQ23_08890"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET9906746.1"
                     /translation="MLHDLPIGVFDSGVGGLSVLRAIREEMPNESVIYFGDQRHIPYG
                     PRPMEQIRNFSEAITNFLLQRNAKIIVVACNTASAAALKYLREKFPDVQFVGMEPAVK
                     PAAEHTQTGKVGVLATPATFQGALYASVVERFANGVELFQDTCPGLVQQIEQGNLDGP
                     ETRNILENALLPMLEKNIDTIVLGCTHYPFVIPLIQQIVGNKVRVIDPAPSVAKQVRR
                     LLEARELRNDSTSKGELRFYTSGDSQVLKSMLPALLGEAGPVEKAEWVNDLRVGV"
     gene            complement(9541..>9978)
                     /locus_tag="VFQ23_08895"
     CDS             complement(9541..>9978)
                     /locus_tag="VFQ23_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906747.1"
                     /translation="VRFYVNGIFHYVSTLFLLDYKRNRKNKLPNPGTIIKVLYPLGLS
                     QLLDPIYQVLDRPFGENLSYGRTILENRNKQFVHGSFSPENVRSLVTDSNIFDDIQRK
                     QFIRNHWDLYDRLILLRLRLLSIMTALNVSFDKYPPQKIFHIK"
BASE COUNT         2620 a         2525 c         2408 g         2425 t
ORIGIN      
        1 agcggacaac gggaatcagg atcgctgagc caatccccag cgtagagagt agcgattgag
       61 tatctgcttc acctggaccc gtggcgttga ttacaattgg gtctccatct gcatccacgc
      121 ggctcaccaa ctgggcagca aaatccgagt catcaaccgg gagagctttt cctcgctgtg
      181 caacaaccga cacgctgcca ttttcaatct gataataagc cacgtttgag cagtgcaggt
      241 gagtggagat tgcggcgatg atcatattca acaatttgct ctgatcagtc tctgcaaaga
      301 gggtctccgt aacattgaac aaaggtcgca aagcctgaac gcgggcggtg tcgcgcttgc
      361 gctgattatc ggtcagggct tgctttgccg cctgaatcag ttcatcgctt tttttgaatg
      421 gttttaggat cagtccatcc actccctgcc gcaatgctcg gattgccgtt tcgaccgtac
      481 cgaagcctgt catgaccaac acagcaatat ccgggaggac catttgcgca cgagagatga
      541 cgtcgaaacc atccacttca ggcatacgga tatcaaccaa tagaaggtcc acgtgattct
      601 tttgaagaaa ttcaattgcc tcgcgtggat tggttaaaga cgtgacctga taatcagcac
      661 gacttaagat tcgattgcat aacaatgcaa tcccaggctc atcatcgaca acaagaactg
      721 aaatagattc cataagaaat attcaccata atcccgacct agtctctcat gtgaaaaaga
      781 gtagcagggc gagtgagtct atattagagc ggtaaccaga cagtaaattt tgctccagca
      841 cccggctgac tcaaaacctc gatctgccca ccatgatctg taataattcc gtacgtgact
      901 gacaacccta aacctgttcc gccttgattt cctttagtcg tatagaacgg ttcaaagata
      961 cgcgcctgat ccagttgagg gattcctacg cctgagtcct gcaccgatac ggtgacccaa
     1021 tcccgtccat cgcggctcgc atttgatgtc ctaatttcca tttctcctcc atcgggcatg
     1081 gcctgcaacg cattatgaac aaggttgatg aggacctgct tcatttgatt tgcatccacc
     1141 aaaacccagg gaagattttt ttcaagatcg atttttaatt cgatcccact aatgtggata
     1201 agatgtttgc tcagagcgac cacatcttca acgacctcat ttaacgaggc ctttgcacgg
     1261 gtgctttcgc tttgccttgc aaaatcgagt aggcggcgaa cgacatctcg agcacggatt
     1321 gcctcccgca tcactgtttc caaatccttc cggctttcac tttcctgcgg catttcttcg
     1381 aggaccagct ctgcaaagcc tgtaacggaa gttaacggat tattcaactc atgcgcgatg
     1441 cctgcggcca tttcgccgac tgccgcaagt ttcgcggcct ggatgagacg attctctgca
     1501 gagcgttgcg cctccatccg tgcgcgtaac tccatttgag tggaacgtaa ttgactgatc
     1561 gtcacctgca atcgctgata ttgatccgca ctggtgataa cgctggcgag gatgccagcc
     1621 aatgattcga gcgccataaa atcgttgtag gtaaaggcgt tgcgtgaact gctctccaca
     1681 tcaataatgc caaggattct ctccccttcg cgaatggcta cgcacatttc agaaccagcc
     1741 tgccagcctt tgatggaccg atagcgctta tcgtgtaaaa catcattgac cataatactt
     1801 tcgcccgtgc cgaacacgta gccagttatc ccacccataa ccgggtattc gaacgatttc
     1861 atcgcgcgct tgaccacgtt ttgactcgtg ccaccaaaac cgccaatggt gagattccct
     1921 ttttcatcgg caatgaatac ggccgccaat tcataagcaa aataacgagc caaaagatct
     1981 gaggtgataa ttgctacttc acgttgatcc gtcagtccaa ttacctgttg gacgacctcg
     2041 tggatcaagc cgagattccg tgcacggccc tctgcttctt cacgaaggcg tgtgtaatcg
     2101 gccaggctgg caaggtgact ggttatgacc gtcatcaaac tttcatcgta caaattgaaa
     2161 gcatttgttc gcaggctttc cacacacaac atcccgatcg tttgacctct gaatcgcagt
     2221 gggacataca atccagagag tgctttgtcg tagaggggta aattatcatc agtctgactg
     2281 tcgctgagtc ggactttctg tccattcgcc agtaagggag taattttgtg ctcttcggca
     2341 cttcgtacgg tcgatgtcac acctgcacct gacccgggca ccagtgtacc tccatcgaca
     2401 gtacgatact cattcaacaa gcggttatcc gaagaaagaa gaaaaagaac aataagttcg
     2461 gtgcgaaagg cgcgcgctaa caaagcgaac aaccgacgtg cgatctgatc cagatttctg
     2521 cccgatgaaa cggtaatcgc gaaatcgttg agcattccca aacgcaacag atgcccggtc
     2581 atttcatcaa acgtgattag ggtctcaatc gcgggtgcaa cttgtgtggc aaaatcgacc
     2641 atacgattcc attcatcacc tttgaaatcg ctggtgcgcc acaagatcaa cgttccaatc
     2701 aaacgctgcc cgatgaccag gggaacacag gcccatagcc gtgtggcaga cttcaagcct
     2761 ttgtgaggga tttgtgacca actctcatca ccgcgatgga caacgatcgg tgttaggttg
     2821 ctggccatac ggcgcagcaa cgtatggtta tcgatggaga gttcgagatc tgccagagaa
     2881 ggagcattcc attgggcacg cacatccaga aagtcgccgc gcaaaacagc cagcaaggct
     2941 ccttgcacgg aaacgagtcg cacaaatgat gtcagtgcac gatccaaaga ccggtcaaga
     3001 tcgtaggggc tttctgaatt caagccaggg atcaacaaag aggcggcgac cgatgacaat
     3061 gcagatgcat cggccggcat gtcttccgtt tgcattccag caaccaccag gcgccagagg
     3121 cgttgcgatt cattcgtcag ttggtctgca cctgccaaaa ccgcacgcga tacccccggt
     3181 aaagggaagg caaacaacct ggccgcttcc aacgtactga gattggatac tgagacagag
     3241 cgactttgcc cgccgcttaa ggccccacat aaccacgaat ctacttcggc cttgcttaaa
     3301 aattttatca gcgctggctg aacctttttt gccaggtgaa aatgagaaat aatgcgccat
     3361 ttccccgctt cacgctccac cagagcagcc cacgttgcat tcgtcaactg gctggcttct
     3421 ttaagttgag cttcaatcgt catttggtgt ggcatgttaa gttaattata cagcagagta
     3481 gcaagatggt atctggctct ccaatggtag aatcaaccta tgactgacct gattgtaatt
     3541 ggcggcggac ttgccggtag cgaggccgct tatcaggctg cacagcgtgg gctgaaggtg
     3601 cagctctttg aaatgcggcc caacctccag actggcgcac accaaaccca ggatctggca
     3661 gaactcgtat gttcaaattc tctgggctca aacctctcgg atcgcgcctc tggcgtgctc
     3721 aagaacgaac tacggatgct cggctccatg ctgctcgaat gtgctgaatc tgcatcactg
     3781 cccgcaggtg gcgcattagc tgttgatcgt gaactctttg cacagcttgt cactcaaaga
     3841 attgaagcac accccaacat agaaatcgtt cgagaagaag caaaagagat tcctgactct
     3901 ccctccatca ttgccagcgg gccgctaact tccactgcat tatcagcggc tattgcagaa
     3961 ttgagcggcg aaaagcattt gttcttcttc gatgcaattg caccgattgt ccaggcagag
     4021 agcatcaata tggaagttgc ctttcgtgcc tctcgttatg aaaaaggcga tctggaggaa
     4081 ggcgattata ttaattgtcc atttacaaaa gaagaatatt acacatttgt caatacattg
     4141 ttggaagccc agcgaatcga gctacgttct tttgaggatg ccatcaaaag cggtgtaaag
     4201 gcagggcatt tctttgaagg atgtttgccg gtcgagatca ttgccgagcg cggggtggat
     4261 tcccttgctt ttggtccgat gcgcccggtc gggttaagag atccacgaac aggcaagcga
     4321 ccctacgcgg tggtgcaatt gcgtcaggat aatcttgcag gcagtcttta taatctcgtt
     4381 gggttccaaa ccaatcttaa gtttcctgaa caaaaacggg tcttccaact gattcccggt
     4441 ctcgagcgtg ccgagtttct tcgctatggt caaatgcatc gcaatacgtt tattgcttcg
     4501 ccaaagcttt tgcgtccaac actgcaacat ttaaaccgcg atgatctttt ctttgctggc
     4561 caaatcactg gcgtggaggg ctatatggga aacatagcca caggtttgct ggcaggctgg
     4621 aatgcagcac gtctgcttca caatgaggaa cccattgtgc ttccacagac aacgatgctg
     4681 ggggcattat gtcactatgt aacgcatgca gacctaaaag attttcaacc aatgaaagcc
     4741 aactttggta ttcttccacc tctggagttc acctccaaaa tcgggaagcg ggaacggggc
     4801 agcgcgtatg ccgagcgcgc gttgacccac ttacaacaaa tgctttctga agagcgtgca
     4861 gacgatagag aacaagtatt gaaaccggcg ggaaatgact aagaaattcg ctctccttta
     4921 tcttgcaatc attggccttc tcttaaccgg tgcaattggc tggtatgttt tcgcgtttcg
     4981 atccgagcca aaggccgata cttcggtatt taacgggcaa cgcgcatacg aagatgtgaa
     5041 aacacaggtc gctctgggcc ctcgatggcc cgggtccgag gggcacgctc aactccagga
     5101 atggatacgc agtgaacttg aagctgctgg ttggcaggtc gagatccagc agtccgaagc
     5161 catgggtcat cctatcgaaa atatcgttgc aaagaaaagc gagacgagtc cgcaaattat
     5221 tttcggcgcg cattacgata cacggatcta cgccgatcat gatcccgatt catcaaaaca
     5281 cgaagaatat gtgccgggtg caaatgatgg tgcgtcaggc gtagcggttt tgcttgagct
     5341 ggcaagaact ttgcctgaag acacgccgcc aatctggctg gtttttttcg acgccgaaga
     5401 caatggcaat atcgagggct gggactggat tctggggtca cgcgaatttg tgaagaacaa
     5461 tactcttgat ccgcaagctg ttgtcattgt ggatatgatt ggtgatgctg atctgaacat
     5521 ttacaaggaa tataactcca accccgaatt gacgaacgaa atttgggatg ttgcgaaaag
     5581 cctgggctat gaggacaagt tcattcccga ccacaagcat tccatgaccg atgatcatac
     5641 ccccttcctg gaagccggca tgcccgccat tgatattatt gatttcgatt atccctattg
     5701 gcacaccaca caagatacgg tagataaagt ctccggcgag agccttgaaa ttgtaggcaa
     5761 aaccctatgg acctgggcga cacagcaaac accacaaccc tgataaaatg gggcttgaga
     5821 cgctgaggag ggtcactccc gacgaagcat tttgagttca tgccgacaaa aaactcatca
     5881 actcctattc aaaaggcgct cgcaaagatt cgacccgcat ggattgcacg cgtcgggcag
     5941 gatctggcgc gcggcatgga ggtacgcgcc gggtttgaag agcagctaaa gcgattcttc
     6001 gaactgcttg tacagtcggt caccaccggt gacccggcct ggatggatcc aatcctgctc
     6061 gattgggcaa aatcgtccac ggaaacagac cttgaagaag ggcaatatca cgtttctttc
     6121 ctgatgaacc ggatgatcgc tttgacgatc caggtgtcgc gtgaatcatt gaccaaacaa
     6181 caagccctcg accttctggc ttccgtcata cctatttaca cctatgggct ggaagtcgtc
     6241 gcacgctacg agatggaaac ccgcgtggcg cacatttcca gcgagatgga aaaagtgcag
     6301 caacgcatgg aaaaagtgga taagagcaag tctgcgttta tctccgtagc cgcccacgag
     6361 ctcaagacgc cgattaccct gatcgaaggt tatgcgtcca tgatggatga cctggcacaa
     6421 gataaaaaca atgggacgct ggggagtttg ttgaccggaa tgaacaccgg tatcggacga
     6481 ctacgaacca tagtggacga tatgatcgat gtctcgttga ttgacaacca gcttctgaca
     6541 ctaaatttcc aaccgattca gatcgctcat acaatcagcg ccatccaaat ggaagttgca
     6601 agtatccttg caaaccggaa gttgagcctc gaggttttgg aattcgacgg aagcaaacaa
     6661 tggatttatg ccgactccac acgcctgatg caggcccttc aaaacgtaat tagcaacgcg
     6721 atcaagtaca cgcctgatgg cggaaaaatc aagatcgacg gacgaacatt gcctggcttt
     6781 atcgaagtaa tcgtaaccga caccggtatt ggaatctcgg ccgaaaacca gtcaatgatc
     6841 tttgaaaaat ttgggcagct tggtcgcgtg gaattacact ccagcgggaa gacaaagttc
     6901 aaaggtggcg ggcccggttt gggcttacct atcgcacgcg gtatcctgga ggcccacggt
     6961 ggctcgatct gggttgagtc agagggatac gatgagattc gatgtccagg atctacattt
     7021 cacatccttg tgcctgcccg cacggatacg cctgatgtgg aaatgaagaa attatttgat
     7081 acacttgata aaaggaaata gatacatttg gcaaaaagaa tcccccaacc cacagttgca
     7141 ccgcgtgaac gcgcgtttct tgtaggtgtt gatttatatc agcaaaaaaa ctttttatca
     7201 ctgggtgact ctctcaccga attggcgcta ctggcagata catcagggct ggacgtcgtc
     7261 ggtgagctga cccaaaagct ggatcgcccc cacgtcaaaa cctatatcgg tcccggcaaa
     7321 gtggaggagt tgaaggcgct cgtcgaggaa acgttgacgc aggtcattat cttcgacgat
     7381 gaactttctc cgcgtcacca acgcgaattg caggaaatca tggggagaaa tgtgcgcgtc
     7441 ctggaccgga ctgcactgat cctcgatatc ttcgctcaac acgctcacac cagcgaaggt
     7501 atgttacagg tggaactcgc tcaatacgaa tactatctac cgcgcctcac aggtcaatgg
     7561 actcacctgg agcgacaagc tggcggcggc ggtggacgtg caggttctac aggtggcgta
     7621 ggcctgcgag gtccgggtga aacacaactg gaagtagaca agcgcgccat ccgtaagcgc
     7681 atttcgcatc tgaagaaaga gctggaaaaa gtgcgagcgc accgcatgag atatcgcgcc
     7741 caacgcaagc gctcccgcat tccaaccgta gccctcgttg gatacacgaa cgctggtaag
     7801 tctacactcc tgaataaact ggcaaaatcg gatgtatttg ttgccgatca gttattcgct
     7861 acccttgacc ccacaacccg tcgcgtggaa ttaccgggcg gttaccaggc attgatcaca
     7921 gataccgttg gtttcatcca aaaactgcca accactttga tcgaatcgtt tcatgcaact
     7981 cttgaagaaa tcgttgaggc agatttgtta ttgcacgtag tggatatttc gcacccgaac
     8041 gcgctaaatc actttgaatc agttcagcag acgctggagg aactcggtgc gaatcatatt
     8101 ccagttgtca cggccttgaa caagatggat aacttgcgtg atcccgaagc cgcaaaagag
     8161 gctgtgcggc gtttctcgaa gtcagtcacg atctcgggac tgaagggaac tgggatttct
     8221 gaactcttaa aactgattca ggaagctctt tatgaaacgt acgctcccat ccttgtacgg
     8281 cttccatacc aacagggggc cctgatctcg ttattccatg aggccggtca agtagagcgc
     8341 atcgaacatg gacgaggcgg cgtgctgatg cagggtcgca ttccgggaag gctgcttgca
     8401 caattcgtac aatggcaggt ttcatcaaat tatcgtgaac cggaactcga agaagaggaa
     8461 gaactatgaa atggctttcc aggcttgtgg atcgtgcgtc agattacttc gcacatcgca
     8521 aagggctgtt acctttaatc ggtatcctgc tggtgatcat caactacgtc ttgcctttta
     8581 tttttggatt ggataatctt attaccggaa gcaatttatt tctgcacctt ggcgtggttg
     8641 tcgccatttt cggaatgatg ctggcgtcgg cgctatagct cccaccgttg acacaacgcg
     8701 cccagcagtg gttaaacacc aacgcgcaaa tcgttgaccc actcggcctt ttccacagga
     8761 cctgcctctc ccaacaaggc tggcaacatt gatttgagca cttgagaatc accagaggtg
     8821 taaaacctga gctcaccttt cgatgtagag tcattcctca gctctctcgc ctcaagcaga
     8881 cgcctgacct gtttcgccac tgatggagcc gggtctatta cccgaacttt attccccact
     8941 atctgttgaa tgagcgggat cacaaacgga tagtgggtac atcccaatac aatcgtatcg
     9001 atattttttt caagcatcgg caagagggca ttctccagaa tattgcgcgt ctctggtcca
     9061 tctaaattcc cctgctcgat ctgttgaaca agtcccggac aagtgtcctg gaataactcg
     9121 acaccgtttg caaaccgttc gacaaccgat gcatacagcg cgccttgaaa cgttgcaggc
     9181 gttgccagca ctcccacctt tcccgtttgc gtatgttccg ctgctggctt gaccgccggc
     9241 tccataccga caaattgcac atcaggaaat ttctcgcgta aatacttcaa tgccgctgca
     9301 gaggctgtat tacaggcaac aacgattatt ttggcatttc gttgaagtag aaaatttgta
     9361 atggcttcgg agaaatttcg gatctgctcc atcggccgcg gaccatatgg gatatgtctc
     9421 tgatcgccga agtaaataac cgattcattt ggcatctcct cccgaatcgc acgcaagacg
     9481 gaaagtccgc ctactccgga gtcgaagacg cctataggca aatcatgcag catgttaagt
     9541 ctattttatg tgaaaaattt tttggggagg atatttatca aaactaacat tgagagctgt
     9601 cataattgat aataaccgaa gtcgcaacag tattaaacga tcatataaat cccagtgatt
     9661 tcgtatgaat tgctttctct gtatatcatc aaaaatattt gagtcagtca ctaaacttcg
     9721 tacattttca ggagaaaaag acccatgaac aaattgtttg tttctattct ccaagatagt
     9781 tcggccgtag ctgagatttt ccccaaaagg acgatcaagt acttggtaga ttggatccaa
     9841 aagctgagat aacccgagag gataaagtac ttttattata gttccgggat taggaagttt
     9901 gttttttcta tttcttttgt aatctaacaa gaataatgtt gaaacatagt ggaatattcc
     9961 attgacataa aatcgaac
//
LOCUS       DASTJP010000294         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_996099, whole genome shotgun sequence.
ACCESSION   DASTJP010000294 DASTJP010000000
VERSION     DASTJP010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996099"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..>3906)
                     /locus_tag="VFQ23_08900"
     CDS             complement(115..>3906)
                     /locus_tag="VFQ23_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9906748.1"
                     /translation="DTAYVLRPHGDELPIVQGVLVDITERKLANEALREHQERQQKIL
                     DAMFVFVGLFSLDGLVLEVNRAPLEATGLKAEEVLGKPFADLPPWAYSTATQIRIREA
                     IRRAALGETVREDLLLPLPNNQFVTMDSIFVPLRDSSGKIIQVVGSGVDITARKQAEQ
                     QLLQMNRLYATLSQINQMIVRAEDQADLLRSACDILVAFGGFSFAWAGLLDESSGDVK
                     PVAAHGLDLENWAFPIVNIHAGEYKHVLLATAIRMAKVMTTENLADDPNLVNFYDLIH
                     KNDYRSAAAVPLSLNGRCIGVLSMVSTEAGVFKDEREIALLEEMQVDISFALEKIQAD
                     HRRQRAETDLRHSEERFYNLFHSNPNAVVLTRFADRRIIEVNDAFTHYTGYSREEALG
                     HNTDELNLWADPQEHARYLTLREIQKQVQDFEWQYRTKAGKVRHALMTTETIQMQGEQ
                     FLLSNSIDITERKHAEQALQTSEKAYANLLKNISGAVYRCRNDTDWTVEYISEGCLAV
                     TGYHPDEIIESRITSLGALMHPEDVEPIWAKCQINLAARKACSNEYRIFHRNGELRWV
                     WDQAQGVYSDSGELIYIEGLLTDITELKEAEEALRQSEARQSFLVRLSDTLRSLHDAE
                     EIKTSVARLLAEQLEVHRVFFTEFESNDENVVQHQDYYTNGNMPTISGRYHIRDYGTF
                     LTEETSSGKPVFVADTETLAAITEIERTQYKVMKVRAFVSIPLTINNRFIAIVGVTQP
                     TPRIWTAGEITLIRETFERTWDAVQRGRAQVALQTAERDYRSIFENAPVGIFQSTPAG
                     RFQKANSTIAQIYGYGSPEEMMESVTDIARQVHGSESLRQRFLNFVNEQGAVTDYEAQ
                     NLRKDGSLIWASINARAVRDEMGQILYYEGFIRDITEHRQREEQIQRQLRSLEALHRI
                     DMAITAGLEMNLSLGLLIEELLSILQVDAASILLFSSQLYTLRYAAGRGFRTNAIQHT
                     QLPLGSGHAGRAAFERRSIHIYDLANNLDELSRSSSLSAEQFKSYIAIPLIAKGKIRG
                     VLEIFHRSFLDQNMDWINFAETMANRAAIAIEDAQLVDELQRSNLELAMAYDATIEGW
                     SRAMDLRDHETEGHTQRVTAMTLELAREMRLDTNEIVHMRRGALLHDIGKMGVPDAIL
                     LKPDTLTETEWEVMRRHPALAYEMLLPIHYLKPALDIPYCHHEKWDGTGYPRGLTGDE
                     IPLAARIFTIVDVWDALTSDRSYRKAWSNERTIQYIREQSGQYFDPRVVEAFLSMLER
                     E"
BASE COUNT          889 a         1060 c          956 g         1001 t
ORIGIN      
        1 cttccagttt ctttatgatt gcatcgacaa agggttgaaa atcacggtct cgctgcacgt
       61 caggcgtaag ggcttcgggt aagcagtccc aaatacataa agtgaccatt tgtatcattc
      121 tcgttcgagc atactcaaga atgcttccac cacccgcgga tcaaaatatt ggccggattg
      181 ttccctgata tattgaatgg ttctctcatt gctccaggcc tttcgataac tgcgatcact
      241 ggttaaggca tcccatacat ccacaatagt aaaaatgcgg gcggcaagtg gaatctcatc
      301 ccctgtcagt ccgcgcggat atccggtgcc gtcccatttt tcgtggtggc aatagggaat
      361 gtcaagcgcc ggcttgagat agtgaatggg caacagcatc tcataggcca aagcgggatg
      421 acggcgcatg acttcccatt ccgtttctgt taacgtatct ggcttcagga gaattgcatc
      481 cggtacgccc attttgccga tgtcgtgcag taaagcaccg cgccgcatat gtacgatttc
      541 gtttgtgtca agcctcatct ctcgagcaag ctccagagtc atggcagtga cacgttgggt
      601 atggccctct gtttcgtggt cacgcaaatc cattgcccgc gaccagccct caatggtggc
      661 gtcgtatgcc atggctaact caagattaga acgctgtaat tcatcgacaa gctgagcatc
      721 ttcaatagcg atggctgccc gatttgccat tgtttctgcg aagttaatcc agtccatgtt
      781 ctggtcgaga aacgaacgat ggaaaatctc cagcacccct ctgatcttgc ccttggcaat
      841 cagcgggatt gcaatatagg atttgaattg ttcagccgaa agactgctgg acctggacaa
      901 ctcatccagg ttattggcca agtcataaat gtgaatactg cgcctttcga aggccgcgcg
      961 gccggcatgc ccgctgccca atggaagttg tgtgtgttga atcgcattgg ttcggaaccc
     1021 gcgcccagca gcgtagcgca gtgtatacag ttgtgaacta aaaagcaaaa ttgaggcggc
     1081 atccacctgg aggattgaaa gtaattcctc gatgaggaga cccaggctga gattcatctc
     1141 caacccggcg gtgatcgcca tatcaattcg atgcagggct tccaaacttc tcagttgtct
     1201 ttgaatctgt tcctctcttt gcctgtgctc cgtgatatct cgaatgaacc cttcataata
     1261 caggatctgg cccatctcat cccgcaccgc gcgggcattg atggaggccc agatcaggct
     1321 gccatccttt ctcaaatttt gtgcttcata atcggtgacc gcaccttgtt cattgacaaa
     1381 attcagaaat ctttgacgta gggactcgct gccatgcacc tgccgggcaa tatccgtcac
     1441 gctctccatc atctcctcag gcgagccata accataaatt tgagctatgg tggagttcgc
     1501 cttttggaat cgccctgccg gagtggactg gaagataccg acgggggcat tttcgaaaat
     1561 ggaccgataa tccctttcgg cggtttgcaa tgcgacctgg gcccgtcctc tttggactgc
     1621 atcccacgtg cgttcaaatg tttccctgat aagtgtgatt tcgccggcag tccagattct
     1681 cggggttggc tgtgtgacgc ctacgatggc gatgaaccga ttgttaatag tcagcggaat
     1741 gcttacaaat gctcgtactt tcattacctt gtattgggtg cgttcaattt ccgtgatagc
     1801 tgccaatgtc tcagtatctg caacaaaaac cggcttccca gacgacgtct cctccgtcag
     1861 gaaggtgcca tagtcacgga tatgatagcg gccggagatc gtaggcatat taccgttggt
     1921 atagtaatcc tgatgctgaa caacattttc atcatttgac tcaaattctg tgaagaacac
     1981 gcgatgcact tccagttgct cagccaataa acgagcgaca gatgttttaa tttcttcggc
     2041 gtcatgcagg gagcgtaatg tatcgcttaa tcgcaccaga aatgattgtc gagcctcgct
     2101 ctgacgtaat gcctcctccg cctctttaag ctcggtaata tcggtcagca gaccctcaat
     2161 gtagatgagc tccccggaat ccgaataaac gccctgcgcc tggtcccaca cccagcgcag
     2221 ttcaccattg cggtgaaaaa ttcgatattc attactgcac gcttttctgg ccgccagatt
     2281 gatctggcat ttcgcccaga ttggctccac gtcttcgggg tgcatcagcg ctccgagcga
     2341 ggtgatacgg ctttcgataa tttcatccgg atggtagcct gtcactgcca ggcagccctc
     2401 gctgatatat tccacagtcc aatccgtatc gttgcggcag cgatagacgg cgcccgaaat
     2461 atttttcaaa agattggcgt atgctttctc gctggtttgc aatgcctgct ccgcatgttt
     2521 gcgctcggtg atatcgatac tgttcgaaag caggaactgc tcgccttgca tctggatcgt
     2581 ctccgtggtc attaaggcat ggcggacttt gcccgctttt gtgcggtact gccattcgaa
     2641 atcctgtacc tgtttctgaa tctccctcag cgtcagatac ctggcgtgct cctggggatc
     2701 ggcccacaaa ttcagctcat cggtattgtg tcccaacgct tcctcccggg agtaaccggt
     2761 gtagtgcgta aacgcgtcat tgacctcaat aattcgccgg tcggcgaacc gcgtcagcac
     2821 taccgcattg gggttcgagt gaaagagatt gtaaaatcgt tcctccgaat ggcgcaggtc
     2881 tgtttccgct ctctggcgtc tgtggtcggc ctgtattttc tcgagcgcaa atgaaatgtc
     2941 cacttgcatt tcttccagca gcgcgatctc acgctcatcc ttgaacacgc ccgcttcggt
     3001 cgagaccatg ctcagcacgc caatacagcg gccattcagg ctgaggggta cagccgcggc
     3061 cgagcgatag tcatttttgt ggatcaagtc gtagaagttc accaggtttg ggtcatcggc
     3121 taggttctcg gtcgtcatca cctttgccat gcggatggct gtcgccagta agacgtgctt
     3181 gtactcgcct gcatgaatat ttacgatcgg gaaggcccaa ttttccagat ccaacccgtg
     3241 ggcagcgacc ggtttgacgt ccccggaaga ctcatccaat aacccggccc aggcgaagga
     3301 aaacccgccg aaagccacta aaatatcaca agcggatcgc agcaggtcag cctgatcttc
     3361 cgcgcgtacg atcatttgat tgatctggct taatgtcgca tacaaacgat tcatttgcag
     3421 gagctgctgt tctgcctgtt tgcgcgccgt aatatccaca ccagacccga ccacctggat
     3481 gatctttccg gatgaatctc gtagtggtac aaaaatacta tccattgtga cgaactgatt
     3541 attgggaaga ggcagaagca aatcttcacg taccgtttct cccaaagcag cacgtcggat
     3601 cgcttcacgt atccgtattt gtgtggctgt ggaataggcc cagggaggca aatctgcaaa
     3661 cggcttgccg agaacctcct cggctttcaa tccggtagcc tccaatggcg cccggttgac
     3721 ctccaaaacc aaaccatcca gcgaaaacaa gccaacaaac acgaacatcg cgtccaggat
     3781 cttttgttga cgttcctgat gctcacgtaa tgcttcgttt gcaagtttcc gctccgtgat
     3841 atccaccagt accccctgca cgatcggcaa ctcgtctcca tgcgggcgaa gaacgtaggc
     3901 ggtatc
//
LOCUS       DASTJP010000295         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_2178,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000295 DASTJP010000000
VERSION     DASTJP010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2178"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2058)
                     /locus_tag="VFQ23_08905"
     CDS             complement(<1..2058)
                     /locus_tag="VFQ23_08905"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906749.1"
                     /translation="MARMALALDFPLTLISAPAGFGKTTLLSQWVSVSKVGNVRERLA
                     WISLENECDLRRFWTYITTALDEIQPGIGKSALALFDSPQPPIHAILRILINAISEAA
                     KDFVLILDDYHHVNDSTIHATLTFFIDHLPSNVHLVVAGRSQPPLSLARWRASNQLYE
                     LREDDLRFTLEEIAVFFNEIKRLNLSSQEITALETRTEGWVAGLQLVALSIQGFDDRS
                     KRSFVSAFAGSQRYILDYLVEEVLQRQPDHIKAFLLETSVLNHLNASLCDAVTGHKDS
                     QSILESLERAHLFTIPLDYEQRWYRYHHLFKDVLHHQLIQTKPDVVLGLHRHAMAWFI
                     DVGRTDDAIRHACAAQEWDQAIKLIEPVIGTAWNRAEIRKIIEWLERLPREQLDSHPD
                     LSLYYSRALLHGGQLEAADQRLQKAEKMLRARLKESSNGDDQLLGTICALRTTVAAVS
                     SEPVNALNLGREALSLLHPDHGDIRAYALNAMGVAHYHLGNLADAARLLAEGGELARK
                     AGVLRSMMVAASFRAEALIGQGQLRPAELILRQALDETAIAQRPTQPWTPASSLICAI
                     YGHLLYEWNRLEECECYLNEAIELGQQHAFSRPLWLAYHTLVRLKLAYGDQNGALAMF
                     KQLQHYQRMHSLPVPVRLIQAKQVRAWLVLGQLEDAEHWARTYNIEGTSSPVFIQEFE
                     SITL"
BASE COUNT          560 a          546 c          551 g          552 t
ORIGIN      
        1 caaggtaata ctctcaaatt cctgaatgaa gacaggcgat gatgtcccct ctatattgta
       61 cgtgcgtgcc caatgttcag cgtcttctaa ttgtcccaat accaaccagg cgcgtacttg
      121 cttcgcctgg atcaagcgca caggaactgg caaggaatgc attcgttgat aatgctggag
      181 ctgcttgaac attgccagcg cgccgttctg atcaccatat gctaacttaa gccggaccag
      241 tgtgtggtaa gccaaccaga gaggacggct aaaggcgtgt tgctgtccta attcaattgc
      301 ctcattgagg taacactcgc actcttccag gcggttccac tcatatagaa gatgaccata
      361 gattgcacaa atcagactgc tagccggtgt ccacggttgg gttggccgtt gcgcgattgc
      421 tgtctcgtcc agcgcctgcc gaaggatcag ctcagctggt ctgagttgcc cctggcctat
      481 cagagcttcg gcgcgaaatg aagcggctac catcatcgag cggaggacac ctgccttccg
      541 agccaattcg cctccttcag ccaataatcg cgctgcatca gccaggttgc ctaaatgata
      601 atgagctacc cccattgcat ttagagcata agcacgtata tccccgtggt cgggatgcaa
      661 tagactaaga gcttcacgac ccaggtttag tgcattgacg ggctcacttg aaacagcagc
      721 aaccgtggtg cgcaacgcgc agatcgtgcc cagtagttgg tcgtcgccgt ttgaagattc
      781 tttcaagcgg gcacgcagca ttttctctgc tttttgaagt cgctgatcgg cagcctccaa
      841 ttgccctccg tgcagcaaag cgcgtgagta atacaatgat agatctgggt gtgagtccag
      901 ttgttcccgc ggcaatctct ccaaccactc gatgatctta cgtatctccg ctcgattcca
      961 ggctgtgccg attacgggtt cgattaattt gatcgcctga tcccactctt gcgcagcgca
     1021 cgcatgtcga attgcatcat ccgttcgtcc gacatcgatg aaccaggcca tggcgtgccg
     1081 gtgtaatcca aggacaacat ccggttttgt ttggattaac tggtgatgca aaacatcttt
     1141 aaataagtga tgatagcgat accatcgctg ctcataatcc agcgggatcg taaataaatg
     1201 ggcgcgttcc aaagactcca agattgattg cgaatccttg tgtccggtaa cagcatcgca
     1261 gagcgaagca ttcaagtgat tgagaaccga agtttctagt aagaaggctt tgatgtgatc
     1321 aggttgtcgt tgcagtacct cttccacaag gtagtcaaga atatagcgct gactgccagc
     1381 gaacgcggaa acaaaactcc gctttgaccg atcgtcaaag ccttggatcg agagggcaac
     1441 gagctgcaag ccggcgaccc aaccctcggt tcgggtctct aaggcagtga tctcttgcga
     1501 cgagagattc agtctcttaa tttcattgaa aaaaactgca atttcttcaa gcgtgaagcg
     1561 caagtcatct tctcgtaact catacaactg attgctcgcc ctccagcgtg cgagtgatag
     1621 gggcggctga ctacgacctg cgacgaccag gtgcacattc gatgggaggt ggtctatgaa
     1681 aaaggtgaga gtggcgtgaa tggttgagtc attcacatga tgataatcat ccaggataag
     1741 tacaaaatcc ttggcagctt ccgaaatcgc attgattaat attcttagaa ttgcatggat
     1801 aggtggctga gggctatcaa acaaggccaa agcggatttg ccaattccag gttgtatctc
     1861 gtctaaagca gtagtgatgt aagtccagaa tcggcgcagg tcacactcat tttccaatga
     1921 aatccatgca aggcgctctc gaacattgcc gactttactg acagagaccc attggctcaa
     1981 caaagtggtt ttgccaaatc ctgcgggtgc agagattaat gtcaacggaa agtccagtgc
     2041 cagagccatc ctagccatca gggtagttct agcaatgagt tccccgtgca gtggtggaat
     2101 tcgcagattg gtttttagaa ggggagagag gatattaacc gataaatttg acgctttcat
     2161 tagctgccaa ccagctttct gaatatggtc gttaatcatc atttttatc
//
LOCUS       DASTJP010000296         5476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1268949, whole genome shotgun sequence.
ACCESSION   DASTJP010000296 DASTJP010000000
VERSION     DASTJP010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268949"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFQ23_08910"
     CDS             <1..584
                     /locus_tag="VFQ23_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906750.1"
                     /translation="INILFHPGHPTDGVLLYDGIDELELASIFDTYAATYSAKLQSVS
                     QTRRWITTRYGLQIVPRWGFKDVPPVTRLIVPGSQARQLASVEISAWESNGNVAQAVF
                     LHADNPDGFAFDAPLQDLAYRENISTAILDAKRLEYRPGTLLTEGSGWPIWLTIRPLL
                     LGMASLAFTKFISGWSRRLSLPRVGRVIVTAEV"
     gene            891..1880
                     /locus_tag="VFQ23_08915"
     CDS             891..1880
                     /locus_tag="VFQ23_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9906751.1"
                     /translation="MNGQNQNRKINSSRSKGQGCLIWLGRIMVLIIALAMLGYIYEPV
                     AEAADAKTYPPPGQLVDVGGHRLHINCTGTGSPTVVIEAGLGDWSTSWASYVQPELAK
                     TTRVCTYDRAGLGWSDASPLPSDAAHFARELHTLLQNANIPGPYVLVGHSLGGFIVRM
                     FAHDYASEVAGVVLVDSMNPRQVTESLSNKLALFSSFEAALARVGIGRLLVKLPAIAS
                     GAPVNGEAYYPLFIRPGSFQTTAQEYKALPVSAAEAAAVTSFGDLPLIVLTAKLNDNP
                     GWPEWQTELLQLSSNSEQLLAENSGHTIQFDEPQAAIEAIVKMVELVRQSAQQ"
     gene            1896..3443
                     /locus_tag="VFQ23_08920"
     CDS             1896..3443
                     /locus_tag="VFQ23_08920"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9906752.1"
                     /translation="MNQRETSWLRKIIFLTIVILTVASSVVRVRPAFAQVTPDNFDAV
                     DAYIRAKMEELRIPGAALVIVQGDQIVHLKGFGVADGSGRPVTPQTPFFTGSTGKSFT
                     ALAIMQLVEAGKIKLDESVQSYLPWFRVADAEASELITIRQLLTMTSGIPQSVGQTQL
                     ANTDRSDTAIENNVRALANMELIASPGERYEYSNANYVTLGMIIQVVSGQSYESYVTE
                     HIFKPLEMRKSFTSKIEAQQNGLAVGYQKWFGIPVASPNLPFTRGSLPAGDLSMSVED
                     FGHYLIAHLNDGNYESRSVLSPSGISELHYPEVQMPGSDDFYGMGWEVQQYQNVQVIG
                     HNGAVPGYTTGMFLVPEKDLAIALVMNTYSPMLGIRVQRLPSSVLRMLLDQQVIPGYE
                     FLYMRIVYALVMLIPILHLVGILATIRGIRIRRRNAPIRSRTQSTLYIALPLIWNAII
                     AYMLLVTLPGAFGSTMPVILLFQPDVGWVAMISGIFAIVWGLLRTGIMISALRRPFER
                     QLYSVKI"
     gene            complement(3440..3937)
                     /locus_tag="VFQ23_08925"
     CDS             complement(3440..3937)
                     /locus_tag="VFQ23_08925"
                     /inference="COORDINATES: protein motif:HMM:NF022606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HET9906753.1"
                     /translation="MTTSILFYTTSIFIGLGATLVFDLWGLFLKYAFKITPSNFCLVG
                     RWLRFLAEGLLFHSKIDSMPQKSAECTAGWIAHYMIGILFAMVFLTLAGNNWIQHPTL
                     IPAVGFGLVTVAAPFFIMQPAFGLGVAASNGPNPKQARIRSLINHIVFGFGLYLFGLI
                     ARFLH"
     gene            complement(3934..4224)
                     /locus_tag="VFQ23_08930"
     CDS             complement(3934..4224)
                     /locus_tag="VFQ23_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906754.1"
                     /translation="MMRNEQKSARDPDQSKVFQIRIQGHLSQQRMNWFEGLIITLEED
                     GNTLLSGPVVDQSALHGILKRIRDLGMPLLSVNSVDRNLENMTKPTQINDGS"
     gene            complement(4221..5132)
                     /locus_tag="VFQ23_08935"
     CDS             complement(4221..5132)
                     /locus_tag="VFQ23_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9906755.1"
                     /translation="MKAIVATKYGGPEVLQLQQVEKPTPKANEILIKVHATTVSAGDF
                     RMRSFTVPPAFWIPARLALGLTKPKQPIYGMELAGEVEAVGKDVTRFKAGDQVFASTL
                     TENFGGYAEYKAFPEHGMVEIKPANITYEEAAALPIGATTALRLLRKGKIQRGQKVLI
                     YGASGSVGTYAIQLAKYFGAEVTGVCSTSSLDMVKSLGADHVIDYTKEDFSTSNERYD
                     IIFDTVAKFPKSQYPKVLAPKGTYVTMAKLSTKEGMENLVFIKELIEAGAINVVIDRC
                     YPLESMVDAHRYVDTGHKKGNVVITVQ"
     gene            complement(5221..>5476)
                     /locus_tag="VFQ23_08940"
     CDS             complement(5221..>5476)
                     /locus_tag="VFQ23_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906756.1"
                     /translation="GIAMFRARILPRWTAALLAVSGPLAGTMFTLLPHQLVQLTSIPM
                     GTAMVWLGYALLTERHQRSAAHAASIAGSSSTRLESSKVI"
BASE COUNT         1317 a         1365 c         1386 g         1408 t
ORIGIN      
        1 taataaacat cctttttcac ccgggtcatc ccactgacgg ggtattactg tacgacggca
       61 tcgatgagct tgaacttgcc tccatttttg acacctacgc tgcgacctac tctgccaaac
      121 tgcaaagcgt ctctcaaacc agacgatgga tcacaacccg atatggttta cagattgtgc
      181 ctcgttgggg tttcaaagat gttccgcctg tcacccgatt gatcgtacca ggcagtcagg
      241 cacgtcaact tgcatcggtt gaaatcagcg cgtgggagtc gaatggaaat gttgcccagg
      301 ctgttttcct acatgcagac aaccccgatg ggttcgcctt tgatgcgccc ttacaggatt
      361 tagcctatcg tgaaaacatt tcgaccgcaa tattagacgc caaacgcctg gagtacagac
      421 cgggaacgtt gctaaccgaa ggttctggct ggccgatatg gttgacaata cgcccgctgc
      481 tgctcgggat ggctagtctg gcatttacta aatttatcag cggctggtcg cgtcgtctct
      541 ccttgcctcg cgttggtcgc gtaatcgtta cggctgaagt ttgacctggc catctcaaaa
      601 gctattgcta aatgtccaaa tatgatagag gcgatcctta attcaaattg attcattgtt
      661 taagaaaatt ttcactttac gaaaaggctc tgtttcacgt acttcggtga tacagggcct
      721 tttttattca aaatgctacg tcatttgacg tacaaaatgt ggtttcaaag aacggtcttt
      781 tgttttaggg acattacatc atgtctcaga tgcgtagatt tgttgggtga aatatcatac
      841 aagaaacaac aaaaaccaaa caagatcaca ttaggttatg gaggctacac atgaacggtc
      901 agaatcagaa tcgaaagata aattcgtcca ggagtaaagg ccaaggttgt ctaatttggc
      961 tgggccgaat tatggttttg atcatagcgt tggctatgct ggggtacatt tacgagcccg
     1021 tagcagaagc cgccgacgcg aaaacatatc cgcctcctgg tcaattggtg gatgttggcg
     1081 gtcatcgcct acacatcaat tgcactggca cgggtagtcc caccgtggtg atcgaggctg
     1141 ggttaggaga ctggtcaacc tcgtgggcaa gttatgtgca accggaattg gccaagacga
     1201 ctcgggtctg cacctatgat cgagcaggtc tgggatggag cgatgcaagt ccacttccct
     1261 ccgatgcggc gcactttgcc agggagctgc atacgctgtt gcaaaacgcc aatattccgg
     1321 gaccctatgt tctggtcggg cattcgctcg gaggctttat cgtacgtatg tttgcccacg
     1381 actatgcttc agaagttgcc ggggttgtgt tggtggattc gatgaacccc aggcaagtaa
     1441 ccgagtcgct gtcgaataag ctagctttgt tctcgtcctt tgaagcggcg ctagcgcgtg
     1501 tcggaatagg gcggttgctt gtgaaactgc ctgcaatcgc ttctggtgcg ccagtgaatg
     1561 gggaagccta ctatcctcta tttattcgcc caggatcttt ccaaacgacc gctcaggaat
     1621 acaaagcgtt gcctgtctcg gctgccgagg ccgctgccgt gacgtccttt ggcgacttgc
     1681 cgctgattgt tctgaccgcc aagctgaatg acaatccagg ttggccggag tggcagactg
     1741 aattgctcca gctatcatcc aatagcgagc agctacttgc ggaaaacagc ggtcacacga
     1801 tccaatttga cgagccgcag gctgctatcg aagccatagt aaaaatggta gaactggttc
     1861 gccagtcagc acagcaatag attgtgggtg atactttgaa tcaacgtgaa acttcctggc
     1921 ttcgaaaaat catttttcta accatagtga tcctgactgt cgcatcatct gtggtcagag
     1981 tccgaccagc ctttgcacag gtcacgcctg ataattttga cgcagttgac gcttacatca
     2041 gagcgaaaat ggaagagctt cgcattcccg gcgcggcgct cgtcattgtg cagggcgatc
     2101 aaattgtcca cctgaagggg ttcggagtag ccgacggttc aggccgtccg gtcacgccac
     2161 agactccatt ctttactggt tcaacaggca agtcattcac tgccctcgcc atcatgcagt
     2221 tggttgaagc cggcaagatc aaattagatg agtccgtaca atcctacctg ccgtggttcc
     2281 gcgttgcaga tgccgaggca tccgaactga tcacgatacg ccagcttcta accatgacga
     2341 gtggaattcc tcaatctgtc gggcagacac agctagcaaa cacagaccgg agcgatactg
     2401 cgattgagaa taacgttcga gccttggcga acatggagct gattgcttcg cctggtgaac
     2461 gttatgagta ctcaaacgca aactacgtta ctctgggaat gattattcag gtggtgagtg
     2521 gccaatcgta tgagagttac gtcacagaac acatcttcaa accactggaa atgcgtaaaa
     2581 gttttacgtc caaaatcgaa gctcaacaaa acggtctcgc cgtagggtac cagaaatggt
     2641 ttggcatacc ggtcgcttca cccaatttgc cttttacacg cgggtccctt cccgctggcg
     2701 atctcagtat gagtgtcgag gattttgggc attacctgat tgcacactta aatgatggaa
     2761 actatgaaag tcgatctgtg ctgtcgccgt cgggaatttc agagcttcat taccctgagg
     2821 ttcaaatgcc gggcagcgat gatttttatg gaatgggctg ggaagtgcag cagtatcaaa
     2881 atgtgcaggt gattgggcat aatggagcag tcccaggcta tacaaccggg atgtttcttg
     2941 tgcctgagaa agatctggcg attgcactcg taatgaacac atatagcccc atgctgggga
     3001 ttcgggtgca acggcttccg agcagcgtct tgcgaatgct gcttgatcag caagtcattc
     3061 ctggctatga attcctttac atgcggatcg tctacgcgct cgtgatgttg atcccaatat
     3121 tgcaccttgt gggcatctta gctacgattc gaggtatccg tattaggcga agaaatgcac
     3181 cgatccgatc ccgaacacaa agcacgctgt atatcgcttt gccgcttatc tggaacgcca
     3241 tcatcgctta tatgctgctt gtgactctgc ccggtgcatt tgggtctacc atgcctgtta
     3301 ttcttctgtt tcaacccgat gttggttggg tggcgatgat tagcggcatc tttgcgattg
     3361 tgtggggatt gctgagaacc ggcattatga tctcagccct gcgccggccg ttcgaaagac
     3421 aattgtattc agtaaaaatc tagtgcaaaa atctggcaat cagcccgaaa aggtaaagac
     3481 caaagccaaa cacaatatga tttattaaac tgcgtatcct tgcctgcttc ggattgggac
     3541 cgttcgatgc tgcgactccc aatccgaatg caggctgcat gatgaagaat ggcgcagcga
     3601 ccgtgactag tccgaagccg acagcaggaa tcagtgtggg atgctgaatc cagttgttgc
     3661 ctgccagtgt aagaaaaacc attgcaaaca ggatgccgat catgtagtga gcaatccatc
     3721 ctgctgtaca ctctgcgctc ttttgtggca tcgagtcaat cttcgaatga aataagagtc
     3781 cttccgccaa gaaccgaagc cagcgcccaa ccagacagaa attagatggg gtgatcttga
     3841 aggcatattt gagaaacagc ccccaaagat caaatacgag cgttgcgcca agtccgatga
     3901 agatggaagt ggtgtagaag agaatggaag tagtcatgag ccatcattga tttgcgtggg
     3961 cttcgtcatg ttttccagat tgcgatcaac agaattgacc gacaacaatg gcattcccag
     4021 atctcgtatt ctcttcaaga taccatgcaa tgcggactga tctactacag gcccgctgag
     4081 gagcgtattg ccatcttctt ccagcgtgat gatcagccct tcgaaccagt tcatccgttg
     4141 ctgactcagg tgaccctgga tccggatctg aaagaccttc gactggtccg ggtcacgcgc
     4201 tgacttctgt tcgttccgca tcattgcacc gtgataacaa cgttaccctt tttgtgtcct
     4261 gtgtccacat agcgatgtgc atccaccatg ctttcaagag gatagcagcg gtcgatcacc
     4321 acgttgatcg cgcctgcctc gatcagctct ttgatgaaca ccaaattttc catgccttcc
     4381 ttcgtgctta gcttggccat cgtcacatag gtccctttgg gtgcgagaac ttttgggtac
     4441 tgtgattttg ggaattttgc aaccgtatcg aagatgatgt catatcgttc attgcttgtt
     4501 gagaaatctt cctttgtgta atcaatgacg tgatctgcgc ccagtgattt gaccatatcc
     4561 aggctggatg tactgcaaac gccggtcact tctgccccaa aatatttggc aagctgtatc
     4621 gcatatgtgc caacacttcc cgaagcgccg tagatcagca ctttctgtcc gcgctggatc
     4681 ttgcccttgc gcaacaggcg caacgctgtc gtcgcgccga tcggaagggc agcagcctcc
     4741 tcatacgtta tattggccgg tttgatttcc accatcccgt gttctggaaa agctttatat
     4801 tcggcgtatc cgccaaagtt ctctgtgaga gttgatgcaa agacctgatc gcctgccttg
     4861 aatcgagtta cgtccttacc gaccgcttcc acttctccag caagctccat gccgtaaatt
     4921 ggctgtttgg gcttcgtaag acctaatgca agtcgagctg ggatccaaaa cgcaggagga
     4981 accgtaaagc tccgcatccg gaaatctccg gcactgacag tggtcgcgtg cactttgatc
     5041 aggatctcat tggctttcgg cgtcggtttt tcgacctgtt ggagttgaag aacttcaggt
     5101 ccgccatatt tggttgctac tattgctttc attctaaaat cctttctttt tacgaataag
     5161 agttgaacag gtcagtatgg agtgatacgg tcggatagac acctcgctct acgcaaagta
     5221 tcaaatcact ttgctggatt ccagcctggt ggaactggag cctgctatgc tagcggcatg
     5281 tgccgcagat cgctggtgtc gttcggtaag gagcgcatac cctagccaga ccatggcggt
     5341 ccccatcggt atcgatgtca actgcacgag ctgatgaggc agcagcgtaa acatcgtgcc
     5401 cgccaacggc cccgatacag caagcagggc agccgtccag cgcggcagga tgcgggcgcg
     5461 gaacatagca ataccc
//
LOCUS       DASTJP010000297         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1238168, whole genome shotgun sequence.
ACCESSION   DASTJP010000297 DASTJP010000000
VERSION     DASTJP010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238168"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFQ23_08945"
     CDS             complement(<1..729)
                     /locus_tag="VFQ23_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9906757.1"
                     /translation="MISPNNLTQNLNEEIITAREGDWRNGAIVYQVLVDRFAPSADLD
                     AKRGLYPAPKILRGWSETPARGRYLHDEKLSSQELDFWGGDFASLSGRLDHIQNLGAD
                     ALYLNPIHLAYTNHKYDAFDFMEISPEFGDREDFLRLVGDVHARGMKVILDGVFNHMG
                     RNAPIFQEAMQNPDSPWRDWFAIGPQYDGGARSWMGFQNLPELNLENPIVRGHIYEDP
                     DSVVRRYLRDGADGWRLDTAYELGP"
     gene            complement(771..1235)
                     /locus_tag="VFQ23_08950"
     CDS             complement(771..1235)
                     /locus_tag="VFQ23_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9906758.1"
                     /translation="MKSTKVYLDPVTFTNWNACIALKLSPDQEKFLPGNLYSIAESQF
                     YEDSRSRAIYNEEDLLVGYALFGRDSFTNKWKIFRIMIDQSFQGKGYGESAMKEIIKQ
                     ISQETDGNEILICYQDANETARRLYAKLGFVEQEIDVEGKVTALLRVCNDVS"
     gene            complement(1455..1841)
                     /locus_tag="VFQ23_08955"
     CDS             complement(1455..1841)
                     /locus_tag="VFQ23_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906759.1"
                     /translation="MTKAHPLTKLQSLLAILLLIPFCVISSVEESKQSVVLFIGMIPF
                     IGLSLITIYLGWLMVFKKEDIIYPHESFSIRFTEKSRGKQAAKNLIAMYTKTSRKMLI
                     GGMNIVSGLLCLISAVVGIVVILRTL"
     gene            complement(1897..>2963)
                     /locus_tag="VFQ23_08960"
     CDS             complement(1897..>2963)
                     /locus_tag="VFQ23_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9906760.1"
                     /translation="GKVTHLILDVMGKYQAIMPLELLQLDRNYVFGIDRNRFDTLIWQ
                     RAANQSGVTACQNFSMTDVVKDAAGRVTGFVGKSGNGSEEVFSADLVVGADGRFSLAA
                     NKFGSKPFEELNDYPSASYHAEWQGVAPYSDTYPHPMIIYQLGKGYSFLCIPIDKGKY
                     IIANYMRTSDTNFGGKIEEHYLRAIQSVPRLREQLKNAHMITRVVGVRRIDNGYRQAY
                     GDGWVLVGDAFHYESPLDGQGFYNALLASKFLAEAIRQWKDGRPWLEAGEEYQKRFYE
                     NTHAMLKQTVKRVQQELYTEPPPFVVNHLMRWMLTDPGYQTQFLRYLSRAIDPNSFTF
                     GPMPGPMLRGLLRDVFKRGQ"
BASE COUNT          742 a          761 c          682 g          778 t
ORIGIN      
        1 gggtcccagt tcataggcag tgtcgagccg ccagccgtcc gctccatcgc ggagataccg
       61 gcggaccaca gaatccgggt cctcgtaaat gtgaccgcgc acgattggat tctcgagatt
      121 caactccggc aggttttgaa agcccatcca ggagcgagcg ccgccgtcgt attgaggtcc
      181 gatggcgaac cagtcccgcc aggggctatc ggggttctgc atggcctctt gaaagatggg
      241 tgcgttacga cccatgtgat tgaagacacc atccagaatc actttcatac cccgcgcatg
      301 gacatcgccg accaggcgca ggaaatcttc ccgatcgccg aactccggag agatttccat
      361 gaagtcaaac gcgtcatatt tgtggtttgt gtacgcgagg tgaatcgggt tcagatagag
      421 agcatcggca cccagattct gaatatgatc cagcctgcca gagagacttg cgaagtcacc
      481 gccccagaag tccagctcct ggctgctcag cttttcatca tgcaaatatc tacctcgggc
      541 gggagtctct gaccaacccc gaagaatttt gggagcgggg taaagtccgc gctttgcatc
      601 cagatcggcg gatggcgcaa agcgatcgac cagcacctga tagacgatag cgccgtttcg
      661 ccagtcacct tcgcgtgctg taatgatctc ttcatttaag ttttgcgtca ggttgttggg
      721 tgaaatcatt gtgctggttt cctgttttct atgatgaatg cgataaatga tcaactaaca
      781 tcattacata cccttaataa cgcggtaact ttaccttcta catcaatttc ctgttccacg
      841 aatccaagtt tagcatatag ccttcgcgca gtctcattcg catcctggta acaaatcaat
      901 atctcgtttc catctgtctc ctgggaaatc tgcttaatga tctctttcat tgcactttcc
      961 ccataccctt tcccctgaaa ggattggtca atcattatcc tgaatatttt ccacttgttc
     1021 gtgaaactat cgcgcccgaa aagggcatac ccaaccaata gatcctcttc gttgtaaatt
     1081 gcccttgacc ttgaatcctc ataaaactga gattctgcaa tcgagtacaa gttgccagga
     1141 agaaattttt cctgatcggg tgacagtttc agagcgatac aagcgttcca gttcgtaaac
     1201 gtgactggat ctaaatatac ttttgtgctt ttcatatcgg ggacgtagtg atggctcaaa
     1261 aaggttgatt ttggataagt ttcttatggg tcaccctgtg gtcaggtgcg cgcattttta
     1321 gacgctgaat aagttgaaga ctcactggct tgtgaaaatt gccatgccca atcctgtacc
     1381 agacgtactc tgtacaaagc ttttgcacga tcaacagcct ctttgcgaac cactacagaa
     1441 ttttaatcag ttcatcacag agtacgaaga attactacaa ttccaacaac ggcactaata
     1501 agacaaagca aacctgaaac tatattcatt ccaccaatta gcatttttct gcttgttttc
     1561 gtgtacattg caatcagatt ctttgccgct tgtttgccgc gtgacttttc tgtaaatcgg
     1621 atgctaaaac tctcatgcgg ataaataatg tcttcctttt tgaataccat caaccaccct
     1681 aaatatatag taatcagact taatcctata aatggaatca tgccaataaa aagcacgaca
     1741 gattgctttg attcttcaac gcttgagata acgcaaaaag gaattaacaa taagatggca
     1801 agcagggatt gaagttttgt tagcggatgt gcttttgtca ttattttaga ttctcatgtg
     1861 cccaactagg agttatgcag cgaaagatta atcaattcac tgaccgcgtt tgaatacatc
     1921 tcgcaagagt ccccgcagca tgggtccggg catcggcccg aaggtgaaac tgttcgggtc
     1981 aatggcgcgg ctgagatagc gcaggaactg cgtttggtac ccgggatcgg tcaacatcca
     2041 acgcatcagg tggttgacca cgaacggcgg cggttctgtg tacaattcct gttggacgcg
     2101 cttcaccgtt tgtttgagca tagcatgtgt gttctcataa aagcgctttt gatattcctc
     2161 tcctgcctcc agccatggac gtccgtcttt ccactgtcgg atggcctccg ccagaaattt
     2221 ggaagccagc aaggcattgt agaaaccctg cccatccaaa ggtgactcgt agtgaaaggc
     2281 atcccccacc aatacccacc cgtcgccgta cgcctggcga taaccattgt caatgcgccg
     2341 cacaccgacg acgcgagtta tcatgtgcgc gttctttagt tgctcgcgca aacgcggcac
     2401 ggactggatc gcccgcaggt aatgctcttc gatcttgcca ccaaagttcg tgtcactggt
     2461 gcgcatgtag ttcgcgataa tatatttacc tttgtcaatc gggatgcaca aaaaggaata
     2521 ccccttcccc agttgataaa tgatcatcgg gtgcggatat gtgtctgagt aaggcgcaac
     2581 accctgccat tccgcatgat acgaggcgct gggatagtca ttcaattcct caaagggttt
     2641 ggagccaaac ttgtttgcag ccaaactgaa gcgcccgtcc gcacccacca cgaggtctgc
     2701 cgaaaagact tcttccgatc catttcctga tttgccgacg aagccggtca cacgaccagc
     2761 cgcatctttg actacatcag tcatggaaaa gttctggcag gctgtgacac ctgactggtt
     2821 tgcggcccgc tgccagatga gcgtgtcgaa cctgttgcgg tcaatgccga agacgtaatt
     2881 ccgatccagt tgaagaagtt caagcggcat gatggcctgg tatttgccca tcacatccag
     2941 aatgagatgg gttaccttgc cct
//
LOCUS       DASTJP010000298         5709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_34948, whole genome shotgun sequence.
ACCESSION   DASTJP010000298 DASTJP010000000
VERSION     DASTJP010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34948"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFQ23_08965"
     CDS             <1..927
                     /locus_tag="VFQ23_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9906761.1"
                     /translation="QGATQTPAVSRTLIRITIRGWMKPINFSRRKPKILLAFVVLGLA
                     IFLFVLQGYTAINYSDPNDPSYSGTFAVENPTPVETLTVLSYNIWFGDNIDVALSELQ
                     EIKSQRELDIILLQEMDEIGTEQIARELEMNYVYFPAAIEPTYDENFGNAVLSRWPIT
                     NSQKLILPHKSFSNRMNRIATRATISLSGSEILVYSLHAESVFTLPRYKQDQYNAVLA
                     DIGSDANLVIVGGDFNSFTETAVKALEEDFWQAGFVRASEGSGSSVMKYGIEVYSDHI
                     FTKGFVVEEAGKMTEATASDHLPIWVTLIPKE"
     gene            1114..1461
                     /locus_tag="VFQ23_08970"
     CDS             1114..1461
                     /locus_tag="VFQ23_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906762.1"
                     /translation="MTTKFGRTTPSQMYMLALAGIDDSIDQVELELSDEERISKITWG
                     RRLLQHMTGKDFGFDLVSWRNFLMTNDDNDKFGYKHPYAFENVDKEIQRSISDASQER
                     LITEIKKRGLDKL"
     gene            1674..2264
                     /locus_tag="VFQ23_08975"
     CDS             1674..2264
                     /locus_tag="VFQ23_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET9906763.1"
                     /translation="MSQELLNLLAARRGHFKFESGHHGDLWLDLDLLFLQPQKLQPFI
                     DALADRLEKYNPAAICGPLVGGALIAHSIAAKLGIEFYYTEPVNIAQPGTLYSVKYRL
                     PKNFESHVSGKKIVVVDDVINAGSAVRATLEELSLQEARPVAIGCLILLGTLAQDYFA
                     KEHIAVESLEALSNNLWMPGDCPLCHSNLPLDEVSR"
     gene            2321..2848
                     /locus_tag="VFQ23_08980"
     CDS             2321..2848
                     /locus_tag="VFQ23_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9906764.1"
                     /translation="MIEITRATTQSHFQAIEELMEEYIAWDTEQTSKLGLDPQVFLSF
                     YYGGTPENLPGKFAPPGGCLLLATLDGKPVGCVGYRQLNEEVCELKRLYVRPQGRGHG
                     IGRLLVTELLKQARSSGYQRVKLETGSFMHEAHQLYQSFGFEFCPPYFEIPEGLREVT
                     NFMELWLDESRKTNN"
     gene            3028..3477
                     /locus_tag="VFQ23_08985"
     CDS             3028..3477
                     /locus_tag="VFQ23_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9906765.1"
                     /translation="MTSAETDLATLSALNKRFIHNFVTNDVPSHDAIIHPLFRTIGTQ
                     GGHMDRATYLVEWATGFDPDVITYWDMRDERITLIGDVALVGATNKWTRVINGVETQG
                     MTCYTDTYIRSGETWLCILAQLTPVSPQNYPPDDTIVVKYLRGVLQP"
     gene            3527..4363
                     /locus_tag="VFQ23_08990"
     CDS             3527..4363
                     /locus_tag="VFQ23_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906766.1"
                     /translation="MAHKSDFKPSLPITLGWLNFATVVEIIAILQLYFFSESTDKFFA
                     WTIQVPLTAAFLGAGFGAGFILVFLYRNETLWADARLALPGVLIFTVLTLVATFIHID
                     KFHIVTSEDRVAVFFSWVWIVIYILAPIVQALAIWQQMRAAGTEPPREHPLPAWLKLS
                     LGLHVAVILLIGIPLFIFPETVSPLWPWALTPLTARAVSAWLVGIGSILAHTIWENDW
                     RRIRGGALAYTVYNLMQIISLFRFPDHIIYGPSFWVYLVILISGLGLGWYGWSTAQKI
                     RR"
     gene            4454..5188
                     /locus_tag="VFQ23_08995"
     CDS             4454..5188
                     /locus_tag="VFQ23_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9906767.1"
                     /translation="MNRKVKAVFAFLCMFLVISISTNVVFFESAQRYYLLLNASKLDP
                     LGLSAFADTPGNMQPPLIVFFGDSRAAEWPAPEKVRNATFLNRGIGNQTTAQLLGRFN
                     VHVAPLHPQIIVLQAGINDLKTIPLFPDQKEVIINNCKANLKQIVDLSLAIDARVIVT
                     TIFPLGRVPIERRPFWSDDVEIAINDVNAFIRTLESDNVIVIETNKVLANADGLVDDQ
                     YSQDFLHLNPAGYAVLNDAIDELFVP"
     gene            5300..5506
                     /locus_tag="VFQ23_09000"
     CDS             5300..5506
                     /locus_tag="VFQ23_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906768.1"
                     /translation="MRQRNWRVILTGVLLSILAAGFFFFMLTVASQSTDPEAFMQTVG
                     QVSGALVGISVAMIVFGLIGKKVK"
BASE COUNT         1489 a         1368 c         1347 g         1505 t
ORIGIN      
        1 cagggtgcta cgcaaacgcc agccgttagc cgtactctaa tcagaataac aatcaggggt
       61 tggatgaaac caatcaactt tagtcgtaga aaaccaaaaa tactattggc gttcgtcgtg
      121 cttgggctgg ccatattctt gttcgtcctc caagggtaca ccgccatcaa ttattcagat
      181 cccaatgatc catcctattc aggtactttt gctgtagaaa atcctacacc agtcgaaaca
      241 ctgaccgttc tttcctacaa tatttggttc ggagacaata ttgacgtggc tctttcggaa
      301 ctgcaggaga tcaagtcaca aagagaactg gatattatcc ttctccagga aatggatgaa
      361 atcggcacgg agcaaatcgc acgcgaactt gaaatgaatt acgtctattt ccctgccgcc
      421 attgagccaa cgtacgatga aaactttggg aatgcagttc tatctcgctg gccgattact
      481 aactcgcaga agcttatcct gccgcacaag agtttttcaa accggatgaa tcgcattgcc
      541 acacgagcaa ccatcagcct ttctggtagt gagattctcg tgtacagctt gcacgctgaa
      601 tctgtattca ctttgccgcg atataagcaa gatcaataca atgccgtctt agcggatatt
      661 ggctcggatg caaatttggt aattgttggc ggtgatttca actctttcac cgagacggct
      721 gtcaaagcac ttgaggagga tttctggcaa gcaggatttg tcagagcatc cgagggtagt
      781 ggctcctccg ttatgaaata tggaattgag gtttattcgg atcacatttt cacgaaaggg
      841 tttgttgtcg aagaagcggg taaaatgacg gaagcaactg ccagtgacca cttgccgatt
      901 tgggttactt tgataccgaa agaatgagaa gagaaaggtt aggacagcta gcaatgcatg
      961 catcagcctt ggtgtgccgc gttttcaagc aggttgtgtg gctttcttga agtaggttcc
     1021 gtcaaaatgg cgttgtctcg ccggccagta cccgaagcgg catgatgcag accattaggc
     1081 ggctatcgcc gaaattcgta aaacaacaaa acaatgacca cgaaatttgg acgcaccacg
     1141 ccttcgcaaa tgtacatgct tgcattagct ggcattgacg attccataga tcaagttgag
     1201 cttgaactct cagatgaaga aagaataagc aagattacat gggggcgtag acttctacaa
     1261 catatgacag ggaaggattt cggattcgat ttggtaagct ggcgaaattt cttaatgaca
     1321 aatgatgata atgataagtt tgggtacaag cacccatacg catttgaaaa tgtggataaa
     1381 gaaatccaac gatccatctc ggatgcaagt caagaacgcc taattaccga aataaaaaaa
     1441 cgcgggcttg ataaacttta aaatcaaagc ttataataat taatgtgttc gttcaaatta
     1501 aataagagta tcaacttgag catctcttct ttatccatca ttaagttttg cgttcaattg
     1561 cgccgcccaa cacagccttc atcgctactc tccgaggctc ttcgacgtac agccgacggc
     1621 aactcaaacc attgggcgct taaactaaac cggacaagaa atttctactt cttatgagcc
     1681 aagaattgct caacttgtta gcggcgagaa gaggacattt caaatttgag tccggtcatc
     1741 acggcgacct ctggctcgac ctggatctgt tgtttttaca gccgcagaaa ctccagccat
     1801 ttatcgatgc gcttgcagat cggcttgaaa aatacaaccc ggcagcaatc tgtggtcctc
     1861 ttgtcggtgg tgcattgatc gctcatagca ttgcggccaa actggggatc gagttttatt
     1921 acacagaacc agtcaacata gcacagccgg gcactttgta ttcggtgaaa tatcgcctgc
     1981 ctaagaactt cgagagtcac gtgtccggga agaaaatcgt cgttgtcgat gatgtgatca
     2041 atgcagggtc ggcagttcgt gccacgctgg aagaattgag cctgcaggag gcgcgtcccg
     2101 tagcaatcgg ttgcctgatt ctattaggta ctttggcgca agattatttt gcaaaagagc
     2161 atatcgcggt ggagagcctc gaagcccttt ccaataactt atggatgcca ggtgattgtc
     2221 cactctgcca ttccaatctt cccctggatg aagttagccg ttgaggatgc accgagtaca
     2281 taatgttctt tttagcaatt caaaagtttg cagttttact atgatagaaa tcacacgagc
     2341 gacgacacag tctcactttc aggcgataga agagttgatg gaggaataca ttgcctggga
     2401 tacggagcag acttccaagc ttggtctgga tccgcaggtt ttcctttcct tttattacgg
     2461 cggtacaccc gagaaccttc cgggcaaatt cgccccgcca ggtggatgct tactcctggc
     2521 taccttggat ggcaaaccgg taggttgcgt tgggtatcgt cagttaaatg aggaggtttg
     2581 cgagctaaaa cgactctacg tccgaccgca aggtcgaggc cacgggatcg gaagacttct
     2641 agtgacggag ctccttaagc aagccaggtc gagtggctat cagagagtca agctcgaaac
     2701 cgggagtttc atgcacgagg cgcatcaact ttatcaatcg tttgggtttg aattctgccc
     2761 tccttatttc gaaatcccag agggtctgcg cgaggtgact aactttatgg agttatggct
     2821 tgatgaatcg aggaaaacga ataactaaca actttactca agtcatgtag cggattgggt
     2881 atttgtcttc tggcgacatt aaaagactgg catattgggg gatttcaaca atgtaatttt
     2941 tcgatgtaga acacgaatct gtactcgcct ttgccagcaa gtaacatatc accatgagac
     3001 atcatctcca tttcaaggag aaatcaaatg acatccgccg aaaccgatct tgcaacactc
     3061 agcgcgctca acaagcgttt tattcacaac tttgtcacca atgatgttcc atcgcacgat
     3121 gccatcattc atcctttatt tagaacgatt ggcacacagg gcggtcatat ggaccgtgct
     3181 acctatttag tggaatgggc aacgggtttt gaccccgacg tgatcactta ctgggacatg
     3241 cgcgatgagc ggatcactct gattggggac gtcgcactcg taggtgccac gaacaagtgg
     3301 actcgagtta tcaacggcgt agaaacgcag gggatgacat gctacacaga cacctatatc
     3361 cgtagtggag agacctggct gtgtattctc gcccagctca ccccggtatc gccccagaac
     3421 tacccgcctg atgacaccat cgttgtcaaa tatttgcgcg gcgtgctcca accgtagtag
     3481 ccagtgtttg gtagtaacga tttccccaat gaagacggat gcttgaatgg cccacaaatc
     3541 tgactttaaa cccagcctgc ctatcacgct cgggtggtta aacttcgcga cggttgtgga
     3601 aatcatcgcc atactgcaac tgtatttctt ctctgaatcc accgacaagt tctttgcctg
     3661 gaccattcag gtgccgctga ccgccgcctt ccttggcgcg gggttcggcg cgggcttcat
     3721 ccttgtcttc ctctatcgca atgaaacgct atgggccgat gcccgccttg cactgcctgg
     3781 cgtactgata tttaccgtcc ttaccctggt agcgaccttt atccacattg acaagttcca
     3841 catcgtgacc tcggaggatc gcgtcgctgt gttcttctcg tgggtctgga tcgtgattta
     3901 catcctggcg ccaatcgtgc aggcgcttgc catttggcaa caaatgcgcg ccgccggaac
     3961 cgaaccgccg cgcgaacatc cacttcccgc ctggctcaag ctttcccttg gactccacgt
     4021 ggcggtcatt ctcctgatcg ggatcccgct ctttatcttt cccgaaacag tctccccgct
     4081 ctggccctgg gcgttgaccc ccttgaccgc gcgcgccgtc tcggcctggc tggtgggcat
     4141 cggctctatc ctcgcgcata ccatctggga aaacgattgg agacgcatcc gcggtggtgc
     4201 gttagcctat acggtttaca atctcatgca gatcatctcc ctctttcgct tccccgatca
     4261 tattatctac ggcccaagtt tttgggtgta cctggtcatc ctcatcagcg gattgggtct
     4321 gggctggtat ggatggtcaa cggcgcaaaa aatccggcgt tgagttgacg ctggccgcat
     4381 ggtgctcttg attttcactg cacctagccc ttcacagacg caaactgcta acagtgaaaa
     4441 atcatttaga tatatgaatc gaaaagtcaa agcggtcttc gctttccttt gcatgtttct
     4501 ggtgatttca atttcaacga atgtagtgtt ctttgaaagt gcgcaacgat attatctact
     4561 attgaatgct tcgaaactgg acccgctcgg cctaagcgct ttcgctgaca ccccaggaaa
     4621 catgcagcca ccgttgatcg tcttctttgg cgattcacgc gcagctgaat ggccggcacc
     4681 ggaaaaagtg aggaatgcaa cttttcttaa tcgaggtatt gggaatcaaa ccaccgcgca
     4741 actcctgggg cgtttcaatg tacatgttgc gcctctgcat cctcaaatca ttgtcctgca
     4801 agctggcatt aacgatttga agacaattcc tttatttccc gatcaaaaag aggtaattat
     4861 caataactgc aaagccaatc tcaagcaaat cgttgattta tcgttagcta tagacgctcg
     4921 agtcattgtt actactattt ttccattggg gagagtgcca attgagagac gtccattttg
     4981 gtccgatgat gtagagattg ccattaatga tgtgaacgct ttcattagaa cattagaaag
     5041 tgataatgtg atagttattg aaaccaataa ggtgctggca aatgcggatg gtcttgttga
     5101 cgatcaatac agtcaagatt ttttacattt gaatccagca ggctatgctg tactaaacga
     5161 cgctattgat gagttgttcg tgccataaga caaagttaac caactcagca agcagcggcc
     5221 gcaaatcatg ggcattctcg gttaaaatca agacgctttc tcatcaccac aaccacttca
     5281 tcgaaggagc gcaaccacga tgcgacaaag gaattggcga gtcattctta ccggagttct
     5341 tctcagtatc cttgctgcag gatttttctt cttcatgctc acggtagcat cacagtccac
     5401 tgaccctgag gcattcatgc aaacggtagg gcaggtatct ggcgctttgg tcggtatcag
     5461 cgttgcgatg atcgtctttg ggctgatcgg taaaaaggta aagtgaaact ttacttttct
     5521 aaatagaata tatctgacaa agtggggcaa tgctgtataa agtcgttcat ctctgtttga
     5581 ttgtgaactt taaagcctaa cacggtttgc tttgaagatt aatcaagtca ccagcaaaat
     5641 gtccagaaaa cgagaacgtt ccaaagagcg tgagcgcaga aatcaaatcg ccagggccgt
     5701 caagaaaag
//
LOCUS       DASTJP010000299         7802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1391792, whole genome shotgun sequence.
ACCESSION   DASTJP010000299 DASTJP010000000
VERSION     DASTJP010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391792"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1587
                     /gene="kaiC"
                     /locus_tag="VFQ23_09005"
     CDS             121..1587
                     /gene="kaiC"
                     /locus_tag="VFQ23_09005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HET9906769.1"
                     /translation="MTPAKRTKQQLKPLPKSPTGIPGMDEITGGGLPKGRPTLIAGGA
                     GCGKTLFAMEFLVNGAIQFDEPGVFVAFEENAEELAQNVTSLGFDLNDLIKRKKLIID
                     YIRVERTEIAETGEYDLDGLFIRLGYAIDRIGARRVVMDTIEVLFAALPNQGILRAEL
                     HRLFRWLKSKGVTAIVTGERGEGMVTRFGLEEYVADCVILLDHRVTEQVSTRRLRIVK
                     YRGSLHGTNEYPFIIGKKGISVLPITSLGLEHKVPSGRVSTGIKELDNMLDGRGFHRG
                     SSILLTGTAGTGKTSLAAAFVDAACWRGERCLYFAFEESPSQILRNMRSIGIDLAPHV
                     KNGLLQFHAARPTIFGLEMHLVTMYDLLIEQKPDIVVMDPITDFTGVGSQSEIKAAIT
                     RMIDLLKTKQITGLFTSYTTLENSADQTIVGVSSLIDAWISLRNLESDGERHRGLFVL
                     KSRGMAHSNQIRSFQLNDHGIQIGVMDLAGRRSASSRS"
     gene            1656..1973
                     /gene="kaiB"
                     /locus_tag="VFQ23_09010"
     CDS             1656..1973
                     /gene="kaiB"
                     /locus_tag="VFQ23_09010"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiB"
                     /protein_id="HET9906770.1"
                     /translation="MKRTKRRTSSSPKEKTWELRLYVAGQTPKSMAAFANLKKICDEH
                     LAGQYNIEVIDLIKKPQLASGDQILAIPTLVRKLPEPIRKIIGDLSDTERVLVGLNLR
                     EQK"
     gene            2008..3201
                     /locus_tag="VFQ23_09015"
     CDS             2008..3201
                     /locus_tag="VFQ23_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9906771.1"
                     /translation="MAKKNPAGTQLQDENLELKSRLEETEETLRAIRQYMVDAFVVTR
                     SNATQIVTLNDTDFPYRMMVESMNEGAVTLIPDGTIFYCNPRFSEMVQIESEKLIGIR
                     FQDLLPSEQKSNFEALFEESGSQGKRGEFCLQSTSGECMPVLLSIYEIITEGTGGISI
                     IATDITERRQAEEKIRTLASQLTMAEQQERQRISQVLHDDLQQRLFAIKTQLTFLTND
                     NEDTGLSPTANADLRQVQRWLSDAIGITRSLSIDLGPMVLQGEGLAEAILWLASQMKS
                     QHSLQIQMDARDNLQLLDNQMRLMLFQTIRELFFNIVKHAATSEATIILEQINEKARI
                     TIRDAGKGFDVGAVMNDAKTAHGLLVVQDRLALLGCSMDVTSEPGKGTQVVIEAPLTA
                     NTGSL"
     gene            3327..4880
                     /locus_tag="VFQ23_09020"
     CDS             3327..4880
                     /locus_tag="VFQ23_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9906772.1"
                     /translation="MPRKNKNQPENINNSNSTRTYSEETEETLQAIRQDMVDALLVTR
                     GNGTRVVTFNDADFPYRLMVESMSEGAVTLIPDGTIFYSNSRFCEMVQTESDLLVGTP
                     FREFIMPEEREAFDTIFSQASLPEARGEFCLKEKAGTCVPVQLSLYPLKKDAVIGISI
                     IITDLTERKRAEEALQKSESLFRLIATNSPDVIFAQDRNLRYTWIVNTVPPLPQEEVI
                     GKRDADLMPIDQALQLTELKQKILKTGISVRQELMLSPSGSQRWFDAIYQPLYDHTQQ
                     IVGIVCYARDITEHIHAEEKIRTLAAKLTVAEQEERQRISQVLHDDLQQRLFAIKAQL
                     SFLNGSDQKHEPSPSPYFSFDEIQSALSEAIAVTRNLSIDISPMILHGEGLTESMKWL
                     SSRMTEQYGLQVQINPKENVDQLDHHVRLVLFQAVRELLFNIVKHAGTSQATITIEQE
                     HPNARVIISDTGKGFDVEAIMKDPQASHGLLMVQDRLGLLGCTMKIISKPGNGTRVIL
                     EAPLEKISE"
     gene            4877..5332
                     /locus_tag="VFQ23_09025"
     CDS             4877..5332
                     /locus_tag="VFQ23_09025"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9906773.1"
                     /translation="MSSQQFKTVISKAGSRVFIALSFNPNDVWGVKGRHHIRGTVNGY
                     QVRGSLGSDGTQYFLPLGAAWRRDCGLEAGAGVEVELWPEGPQSESLSADVAAALDAE
                     LQAKAFFESLATFYRNTYIKWIESAKRPETRSARIHEMIQLLKAGKKQK"
     gene            5476..5910
                     /locus_tag="VFQ23_09030"
     CDS             5476..5910
                     /locus_tag="VFQ23_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9906774.1"
                     /translation="MTGFRLENSFGYLINLSAQRLKYELHQTFQERGYDVTPEQWVVL
                     NRLWEQDGLSQVELAERTFKDKPSTTRILNLLEKKSLVVRRPDEKDGRVLRVYLTASG
                     WEIKEQLVSCADAVLQKASRDFTKEEVAQFKLSLNKLLRNIE"
     gene            5972..6187
                     /locus_tag="VFQ23_09035"
     CDS             5972..6187
                     /locus_tag="VFQ23_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906775.1"
                     /translation="MKFSNAKLRIWLRAFHLAGMIPMGIVIYTALDQPGILLQIVRLA
                     VFPSFIMTGLIMWQMPRLSKWLKRSPA"
     gene            6270..6713
                     /locus_tag="VFQ23_09040"
     CDS             6270..6713
                     /locus_tag="VFQ23_09040"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HET9906776.1"
                     /translation="MTFDASYKEQNRASTERIRAMVAGLSDEEMQTKVGEHWTVVITL
                     AHLAFWDKRVMHILEMTERDGKLFDLEIDVVINDLSLQLWEAIPPQAAAHICIETSEA
                     LDKRLEDFPPQLLEELYNHNKRWVVRALHRNEHLDDADAALKTAA"
     gene            6722..7273
                     /locus_tag="VFQ23_09045"
     CDS             6722..7273
                     /locus_tag="VFQ23_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9906777.1"
                     /translation="MTNPKTIILETKRLLLRLLVMEDLDNLFALYSDPEIRKYFPEET
                     LNYEETKEELEWFLNGHPRHPELGLWATILKETGQFVGRCGLLPWTIEGQDEVEIAYL
                     IARDHWRKGLGSEAAQGILHYAFESLGLSRLICLIDKDNIASIKVATGMGMTFEKEIV
                     DEMGPAQVYSIHKHVLNEANPSA"
     gene            7251..>7802
                     /locus_tag="VFQ23_09050"
     CDS             7251..>7802
                     /locus_tag="VFQ23_09050"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HET9906778.1"
                     /translation="MKQIHLPEEVINKLIRWAEQRDSIRAMLLTSTRAVPNATVDDLS
                     DYDVILIVDNIHPYYEDRSWLEDFGEVLVEYWDPLYRDPDHSIEMFANVIQYADGLKI
                     DFTLWPVELFRKVTQASTLQPELDAGYRILLDKDHLTEAMQQPTYKAYIPTPPTFAVY
                     QKTIEDFFSDVPYVAKCLLRNELF"
BASE COUNT         2149 a         1825 c         1981 g         1847 t
ORIGIN      
        1 agggataact cacaatttgc tgattcaata gcggttaatc atcattgtcc cttaatacca
       61 ttaactgaat cttctgtgaa agacctgcac ccttctggac gaatgtcaag aggaattttt
      121 atgacgccag caaaacgaac caaacaacaa cttaaacctt tgcccaaatc tccgactgga
      181 attcccggta tggacgaaat cactggcggt gggctgccaa agggcaggcc gactttgatc
      241 gctggcggcg cgggctgcgg caaaaccctc ttcgccatgg agtttttggt caatggagcc
      301 atccagtttg acgaacccgg agtttttgtg gcatttgagg aaaatgccga agagctggct
      361 caaaatgtta cctcactggg tttcgattta aacgacctga tcaaacgcaa aaaattgatc
      421 attgactata ttcgcgtcga acgaacagag attgcagaaa caggggagta cgacctggat
      481 ggtttattca ttcgcctggg ctacgcgatt gatagaattg gcgcccgccg agtggtcatg
      541 gataccattg aagtgctctt cgcggcactg ccaaaccagg gcattttgcg agccgaattg
      601 catcgcctat tccgctggct caagagcaaa ggagtaacgg cgattgtgac cggagagcgc
      661 ggtgagggaa tggtgacgcg ctttggcctg gaggaatacg tggcagactg cgtcatcctg
      721 ctggatcacc gcgtgacgga acaggtttca acgcggcgat tgcggattgt taagtatcgc
      781 ggcagcctgc atggcacaaa cgaatacccg ttcattattg gaaagaaagg catctcagta
      841 ttgcctatca cttcattggg acttgagcac aaggtacctt caggacgcgt ctcgactggg
      901 atcaaggaac tagacaatat gctggacggc cgagggtttc atcgcggcag cagcatccta
      961 ctcacaggga cagcgggtac agggaaaact agtctcgcag ccgcttttgt agatgcggcc
     1021 tgctggcgag gggaacgctg cttgtatttc gccttcgaag aatcaccgag ccagattctt
     1081 cgaaacatgc gttccattgg cattgacctg gcacctcatg taaaaaatgg gctgctgcaa
     1141 ttccatgctg cccgcccaac catctttggc cttgaaatgc accttgttac aatgtacgat
     1201 ttgctcattg aacaaaaacc agatatcgta gtcatggatc ctattactga ctttaccggg
     1261 gtgggaagcc aaagtgagat taaagccgcc atcacacgaa tgatcgacct cctgaagacc
     1321 aaacagatca cggggctttt caccagttat acaacattag agaatagcgc cgatcaaacc
     1381 atcgtgggtg tttcatcgct gatcgatgcc tggataagcc tgcgcaacct ggagagcgat
     1441 ggggaacgtc atcgcggttt gtttgtgctg aagtcacgtg gaatggctca ttcgaatcag
     1501 atccgttctt tccagttgaa cgatcatgga atacaaattg gcgtgatgga cctagccggg
     1561 cgtcggtctg cctcgagtcg atcttaaaag gcagttcaaa cgtatctggc tgttattgcg
     1621 gtgttataaa tataaatcat aagaggaaac cagcagtgaa aagaacaaag cgacgcactt
     1681 catccagtcc caaggagaaa acctgggaac tgcgcctcta cgttgccggg caaacgccca
     1741 agagcatggc ggcgttcgcc aatctcaaaa agatttgtga tgagcatctg gcagggcaat
     1801 acaatatcga ggtgattgat ctgattaaga aaccgcaatt ggcaagcggt gatcaaatcc
     1861 ttgccatccc aactctggtg cgtaaattac cagagccgat ccgaaagatc atcggtgatc
     1921 tctcagatac ggaacgtgta ttggtcgggt tgaatttacg cgaacaaaaa tgagaccgga
     1981 agttaccgct cgttgaaatg gtgaaacatg gctaaaaaaa atccggctgg cacgcaactc
     2041 caggacgaga accttgaact aaagagccgt ctcgaagaaa cagaagaaac tctgcgtgcc
     2101 attcgccagt atatggtgga tgccttcgtg gtcacccgct cgaacgccac acagattgtc
     2161 accttaaatg atacagattt tccgtatcgc atgatggtcg aatcgatgaa cgagggcgca
     2221 gtcaccctga tccccgatgg aacgattttt tattgcaacc cgcgtttcag tgaaatggtt
     2281 cagatcgagt ccgaaaagct gatcggaatc cgctttcagg atttacttcc atcggaacag
     2341 aaaagcaatt tcgaagcgct ttttgaggag tccggttcgc agggaaaacg aggagagttt
     2401 tgtctccagt ctacgagtgg ggaatgtatg ccggtgctat tatccattta tgagatcatt
     2461 acggagggta cgggcgggat ttcgatcatc gcgacagata tcaccgaacg ccgtcaggcg
     2521 gaggaaaaga ttcgaacact cgcatcgcaa ctgaccatgg cagagcagca ggaacgtcag
     2581 cgtatctcac aggttttgca tgacgatctg cagcagcggc tctttgccat caagacgcaa
     2641 ttgaccttcc tgacaaacga taatgaagac actgggctct cgccaactgc gaacgcagat
     2701 ctgcgacagg tacagcgctg gctctcagat gcgatcggca ttactcgcag cctcagcatt
     2761 gacctgggtc cgatggtgct gcagggtgaa ggcctggcgg aggcgattct ctggttagcc
     2821 tcccaaatga aatcgcagca tagcctgcaa atacagatgg atgcgaggga taatctccaa
     2881 cttctggata atcagatgcg cttaatgtta tttcagacaa ttcgcgaact tttctttaat
     2941 atcgttaaac atgctgctac ttcagaagcc acgatcattc tggagcagat caatgaaaag
     3001 gcacgaatca cgatccgtga tgctggaaaa ggatttgatg tcggcgcggt catgaacgat
     3061 gcgaaaactg ctcatgggtt gctcgtggtt caagaccgtt tggcattgct gggctgtagc
     3121 atggatgtga catcagaacc aggcaaagga acccaggttg tgattgaggc cccgctgacg
     3181 gcaaatactg gctccctgta atttggaaac aggaatctcc ccttaaaaca atctaaatcg
     3241 tgcttttcag tcacgaaatg tttgaaagtt tgcgtggcat ctcgccattt gtttcgttta
     3301 acaattaaaa gccgaaaagg tgaaggatgc ccaggaaaaa caaaaatcag ccggaaaaca
     3361 taaataattc caattcaaca agaacatatt ctgaagaaac cgaggaaaca ctacaagcga
     3421 ttcgtcagga tatggtcgat gcattactgg taacacgcgg gaacgggacg cgtgtggtga
     3481 ctttcaatga cgcggacttc ccttaccgtc tgatggtcga gtcgatgagt gaaggcgcgg
     3541 tgacactgat cccagacggg acaatttttt acagcaattc tcgcttttgt gaaatggttc
     3601 aaacggagag tgatcttctg gtaggtacgc cgttccggga gtttatcatg ccagaagagc
     3661 gcgaagcatt cgatacaatt ttttcacagg ccagtttacc agaggcgcga ggggagttct
     3721 gcctgaaaga gaaggctggc acgtgcgtac ctgtgcaatt gtcactgtac ccactgaaaa
     3781 aagatgcggt catcggaatt tccatcatca tcacagacct gaccgaacgt aaacgtgcag
     3841 aggaagctct ccaaaaaagt gagagcctct ttcgtctcat tgccacgaac tctccggatg
     3901 taatttttgc ccaggatcga aatctccgat atacatggat cgtcaacacc gttccacctc
     3961 ttccacagga ggaagtgatc gggaagagag atgcagattt gatgccgatt gaccaggcac
     4021 tacaactcac cgaactcaaa caaaaaattc ttaagaccgg gatcagcgta cgacaggaat
     4081 tgatgctctc ccccagcggc agccaacgct ggttcgatgc gatctatcag ccgctgtacg
     4141 atcacacaca gcaaattgtt ggcattgtgt gttatgctcg cgacattaca gaacatatcc
     4201 atgcagagga aaagatccgt acgcttgccg ccaaactaac cgtggcagag caggaggaac
     4261 gtcagcgcat ttcacaggta ttgcatgatg atctgcaaca gcggctgttc gccatcaagg
     4321 cacagttgtc tttcctcaac ggctcggatc aaaaacatga accttctcca tctccctatt
     4381 tcagcttcga tgaaatccag tccgctcttt cggaggcgat cgccgtcacc cggaacctga
     4441 gcattgacat aagcccgatg atcctgcatg gagaagggtt gaccgaatcc atgaaatggc
     4501 tatcctcgcg catgacagag caatatgggc tacaagttca gatcaaccca aaagaaaatg
     4561 tggaccaact cgatcatcat gtgcggctgg tattatttca ggcggtgaga gaactgcttt
     4621 ttaacatcgt caaacatgcg ggcacatccc aggcaaccat taccatcgaa caggagcatc
     4681 cgaacgccag agtcatcatc agtgataccg gcaaaggctt tgatgttgaa gcgatcatga
     4741 aagatccaca agcctcgcac ggtttgctga tggttcagga tcgactgggg ctgctggggt
     4801 gtacgatgaa aataatttcc aaaccaggca atgggacgcg cgtcatcctc gaagcgccac
     4861 tggagaagat ttcggaatga gctctcagca attcaagact gtcatcagta aggcagggtc
     4921 gagagttttt atcgctctgt cgttcaaccc aaacgatgtt tggggggtca aaggccgaca
     4981 ccatattaga ggcactgtga atggttatca ggtgcgtggc tccctcggtt cggatgggac
     5041 acagtatttc cttccgttgg gcgctgcctg gcgccgggat tgtggtttgg aagctggtgc
     5101 cggagtcgag gtggagttat ggcctgaagg tccgcagtct gaaagcttat cagcagatgt
     5161 ggctgccgcg ttggatgcag agctgcaagc caaagccttc tttgagtctt tggcaacgtt
     5221 ttatcggaac acctatatca aatggattga gagtgccaaa cgaccagaaa ctcgttcagc
     5281 ccgaattcac gaaatgatac agcttctgaa agctggcaag aaacagaaat aagatagttg
     5341 atataagctt tttcgggggt aatttattta atgactcagc cattccacct actaaatttt
     5401 ggacttgaca caatcagcag aacaatgcta aaatagttgt catgacaact attttcgatc
     5461 aggaacaccg aaacaatgac tggctttaga ctggaaaact ccttcggata cttgatcaat
     5521 ctctcggctc aacgcctgaa atacgaactg caccaaacgt ttcaggagag agggtacgat
     5581 gtcacccctg aacaatgggt ggtgctcaac cggttatggg aacaggatgg gctttcacag
     5641 gtggaactgg ctgaacgaac ctttaaggat aagcccagca ccactcgtat tctcaacctt
     5701 ttggaaaaga aaagcctggt ggtgcggcgg cccgatgaga aagatggtcg tgtactgcgt
     5761 gtgtacctga cagcctcagg atgggagatc aaagaacagc ttgtatcctg tgcggatgcg
     5821 gttctgcaga aggcaagcag ggattttacg aaagaagaag ttgcacaatt caaattgtcg
     5881 ctcaataaac tgctgcggaa catcgagtaa cgttcttttt ttaacataat agttgtcgta
     5941 acaaccattt tatgtaataa ggagactgaa aatgaaattc tcaaatgcaa aacttcgaat
     6001 ctggctacgt gccttccacc tggcgggaat gattcccatg ggaatagtaa tttacacggc
     6061 gttggatcag cctgggatct tgcttcaaat cgtccggttg gctgtgtttc cgagtttcat
     6121 catgaccggc ttgattatgt ggcaaatgcc acgcctgagc aaatggctca agcgctcccc
     6181 tgcataagta accgatcgcg agagttcttt gtcggggatg tggaaggaca ggcttataat
     6241 tcttaacaca ttccaacatg gagactctca tgacattcga tgcatcctat aaagagcaaa
     6301 atcgagcctc gaccgaacgt atccgcgcca tggtggccgg cttatctgat gaagagatgc
     6361 aaacaaaagt cggcgaacac tggaccgtag ttatcacact tgcgcatctg gcattttggg
     6421 ataagcgcgt gatgcacatc ctggaaatga ccgaacgcga tggcaagttg tttgatttgg
     6481 aaatcgacgt cgttatcaac gatctgtcgc tgcagttatg ggaagcgata ccgccacagg
     6541 cagccgcaca catctgcatc gaaacttcag aggctttgga taaacgcctg gaagactttc
     6601 ctccacaatt acttgaagaa ctttataacc acaataagcg ctgggtggtg cgcgcgctgc
     6661 accgcaatga acatctggac gacgcagatg cggcgttgaa aacagctgca tagtctttat
     6721 gatgactaat ccaaaaacaa ttatcctcga aacaaaaaga cttctactaa ggcttctcgt
     6781 tatggaagat cttgataacc tttttgctct gtacagcgac cctgaaataa gaaaatattt
     6841 tccggaagag acattgaatt acgaggaaac caaagaagag ctggagtggt ttctgaacgg
     6901 gcatcccagg catccggaac tggggttgtg ggcaacgatc ctcaaggaga caggtcaatt
     6961 tgttgggcgc tgtgggttgc ttccctggac cattgaaggt caggatgagg tcgaaatcgc
     7021 gtatctaatc gcaagggatc attggagaaa ggggctgggt tccgaagccg cgcaaggcat
     7081 tcttcattat gctttcgaat cgctgggatt atctcggttg atctgcctga tcgacaaaga
     7141 caatatcgcc tcgatcaaag tggcgacagg catgggcatg acctttgaaa aagaaatcgt
     7201 tgatgagatg ggtcccgcgc aggtatattc aatccacaag cacgtactga atgaagcaaa
     7261 tccatctgcc tgaagaagtc atcaacaaat tgattcggtg ggcagaacag cgcgactcga
     7321 ttcgcgccat gctattgacc agcacgcgcg ctgttccgaa tgccacagtg gacgatttat
     7381 ctgattatga cgtgatcttg atcgtggata acattcatcc ttattacgaa gatcgaagct
     7441 ggctggaaga ctttggcgag gtgctggtcg aatactggga tcccctctat cgcgacccgg
     7501 atcacagcat tgaaatgttc gcgaatgtta tacagtatgc agatggcctc aagattgatt
     7561 ttacgctctg gcccgtggaa ttatttcgga aggttaccca ggcatcgaca cttcagcccg
     7621 agctggatgc aggctatcgc attctcctcg acaaagatca tcttaccgag gcgatgcaac
     7681 agcccaccta caaagcctac atcccaaccc cgccaacatt tgcggtgtat caaaaaacaa
     7741 ttgaggactt ctttagtgat gtgccgtatg tcgcgaaatg tcttttgcgc aatgaattat
     7801 tc
//
LOCUS       DASTJP010000300         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1573268, whole genome shotgun sequence.
ACCESSION   DASTJP010000300 DASTJP010000000
VERSION     DASTJP010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573268"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1504)
                     /locus_tag="VFQ23_09055"
     CDS             complement(29..1504)
                     /locus_tag="VFQ23_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HET9906779.1"
                     /translation="MEERKDFGHAAIQGTAWRFVSYFGGKFMVFLSTIFLARLLAKDD
                     FGIVGYALTAVALLDVASDLGVAEAVVYFEKDNKKYSTAFWISLIIGLVLFGLSWILA
                     PLLVIYFRDDRVLEVSRVMALTFPLTALGSTHEAILRKNLAFERTTVPVFLRAVGKGL
                     ISIVLALMGFGAWSLVWGQLGGTLISSILLLVLTPWRPSLEFDFASAKSLLSFGVKDI
                     GANFLAMILLNLDYWLVGRYLGAEALGVYTLAYRLPELLILQFARIISQVNFPIYTKM
                     RETNGSLARGFWKTTAYVSLLTIPLGIGLALLARPFTIVFLSEKWLDAVPVLQGIALY
                     AMFLSLIHNATSAYKAEGNFRVITGLSVVRLLLLFPALFWATSVMGSIVVVGWTHAGV
                     AFLSAVIGLIVAARMLRLPLRDLFASIWPAVLAGISMGIVVYIILGLASNLSAIQQLL
                     LTVPAGAFVYVLILWFSNRELMLDVIERLRSAVRRRQGKYA"
     gene            complement(1520..>2589)
                     /locus_tag="VFQ23_09060"
     CDS             complement(1520..>2589)
                     /locus_tag="VFQ23_09060"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9906780.1"
                     /translation="ADNSTLALLKLQKFLENAILAFVVVFFVQKPDSFRRIIWTLLLA
                     GIFMGTISVFQSLTSTYGNSYFGFGGWTQSTTGGTSNYRLTGPYGNPNGYAQVLVLLV
                     PLALDRLWHEKDLRMRLLAGYALAVCALTIFFTYSRNGFLTLVFTLGFLVVMRRPRIM
                     PLALSAAMGIILIQFLPASYTERITTLFQFSSGARSRVSDVSFQGRISENIAAWRMFQ
                     DNPIFGVGLDNFQVNYQTYSRKIGLDSRRDPRAPASFYLELLSEQGIIGFSIFMTFMA
                     IIFRNLWQARELFRFLGMNTEEYMSLAFMSGLAGYMVFYISKNSAYPNVFWILLGIAF
                     SIAQVAENSANALRSTSRRLQ"
BASE COUNT          830 a          666 c          531 g          562 t
ORIGIN      
        1 catcacaacg ccgttcgcca ttctgaatct acgcgtactt tccctgtctg cgtcgaactg
       61 cagatcgtag tctttcaatg acatccagca tcagttcccg gtttgaaaac cataagatta
      121 agacatatac aaaagcaccc gcaggcacgg tcagcagcag ttgctgaatc gcagacaagt
      181 tgctcgccaa acccaaaata atatacacca caatacccat ggaaattcca gccaggactg
      241 ctggccagat cgaggcaaac agatcgcgta atggcaaacg caacatgcga gccgcaacaa
      301 tcaatccaat aacggcgcta aggaaggcaa cccctgcatg cgtccaaccc acgactacaa
      361 ttgaacccat aacggacgtc gcccaaaaca aggcaggaaa caacagtaag agacgaacta
      421 cacttaagcc agtaattaca cgaaagtttc cctctgcctt ataggcgctc gttgcattat
      481 gtatcagtga taaaaacatg gcgtataacg caattccctg aagcaccgga acagcatcca
      541 accatttttc ggatagaaac acaatagtaa agggtcgagc taagagagcc aatccgatgc
      601 ctaagggaat ggttagaagc gagacatatg cggtagtctt ccaaaatcca cgtgccagac
      661 tgccattcgt ttcacgcatt tttgtataga tggggaagtt tacctggctg ataattcgcg
      721 caaattgaag aatcaacaac tccggcaaac gataggcaag cgtataaacg cccaacgctt
      781 ctgcgcctaa ataacggcct accaaccagt agtccaagtt gagcaatatc atcgcaagaa
      841 aatttgcgcc gatatctttc acaccaaagc ttaataagga ttttgcgctc gcaaagtcaa
      901 attccaaaga gggtcgccag ggagtcaata ccaacagtaa gatcgatgaa atcaacgttc
      961 ccccaagttg tccccatacc aggctccagg cgccaaatcc catcaacgca aggacgatcg
     1021 agatcaatcc ttttccaacg gcacgcagaa aaacaggaac cgtggttcgt tcgaacgcca
     1081 gattcttccg caggatagcc tcgtgggtac ttcctaatgc agtcagagga aaagtaagtg
     1141 ccatcacccg gcttacttca aggacgcgat catcgcgaaa gtagatcact aggagcggtg
     1201 ccaaaatcca tgacaagcca aacagaacca gaccgatgat caaactgatc caaaatgcgg
     1261 ttgaatactt cttgttatcc ttttcaaaat atacaacagc ctccgcgact cccaggtcac
     1321 tagcaacatc aagtaaggca accgcggtca gtgcataccc tacaattcca aaatcatctt
     1381 ttgccagcaa tctcgctaag aaaatggtgc tgagaaacac cataaatttc cccccgaaat
     1441 aggatacgaa acgccatgcc gtgccttgta ttgctgcatg gccgaaatct tttcgctctt
     1501 ccattaaatg caggattccc tactgcaaac ggcgcgaagt tgatctcaac gcatttgcac
     1561 tgttttcagc gacttgagca attgaaaaag caattcccaa taatatccaa aagacattcg
     1621 gataagcgct attttttgaa atataaaaaa ccatatatcc ggccaatccc gacataaaag
     1681 ccaggctcat gtattcctca gtattcatcc caagaaaccg gaataattca cgtgcctgcc
     1741 aaaggtttcg gaagataata gccataaatg tcataaaaat ggagaagccg ataattccct
     1801 gttctgaaag caattcaaga taaaaactgg cgggtgcgcg tggatcgcgc cttgaatcca
     1861 gaccgatttt tcgggaatac gtttgataat tcacctggaa gttatctaaa ccgacaccaa
     1921 agatcggatt atcctggaac attctccagg ctgcaatatt ttcactgatg cgaccctgaa
     1981 aggaaacatc agaaactcga ctccttgctc ccgaagaaaa ctgaaacaat gtagtgatcc
     2041 gttctgtata agaggcaggc aaaaactgaa tcaagatgat gcccattgca gcactgagtg
     2101 ccagcggcat gatccttggt cgcctcatta caaccaaaaa ccctaaagta aaaactaaag
     2161 tgagaaagcc gtttcgtgaa tacgtgaaga atattgtcaa ggcacacact gccagggcat
     2221 acccggcgag caggcgcatt cgaaggtcct tttcatgcca caagcgatcc agcgccagag
     2281 gaactaatag aaccagcact tgagcataac cattcgggtt tccatagggg cccgtgaggc
     2341 gataattgct ggttcctccg gttgttgact gagtccagcc tccaaatcca aaataactat
     2401 ttccgtaggt agacgtcaga ctctggaaga cgctgatcgt tcccataaaa attcccgcca
     2461 ataacagagt ccaaattatt cttcgaaacg agtcgggctt ttgaacgaag aacacaacga
     2521 caaaagccag aatcgcattt tcaaggaatt tttgcaactt cagcaaggcc agcgtcgaat
     2581 tatccgccg
//
LOCUS       DASTJP010000301         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1382383, whole genome shotgun sequence.
ACCESSION   DASTJP010000301 DASTJP010000000
VERSION     DASTJP010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382383"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..445)
                     /locus_tag="VFQ23_09065"
     CDS             complement(17..445)
                     /locus_tag="VFQ23_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906781.1"
                     /translation="MSKPIEFQSPNKKHKAVLTPVGQVQADHSYYSLSIDGLPHSFAG
                     RIFGRVCLWSPESRFLVVQEWKESDEGGTPKFCQLLIIDLSARRECIIANVEGAKGSI
                     LPESFIGDSLMYTVIYYGQFGMTKSFESRFQYLNGWQIIK"
     gene            complement(476..1597)
                     /gene="tgt"
                     /locus_tag="VFQ23_09070"
     CDS             complement(476..1597)
                     /gene="tgt"
                     /locus_tag="VFQ23_09070"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9906782.1"
                     /translation="MFKFTITARNKRARTGIFTTPHGNLITPVFAPVGTQATVKTLTP
                     VQVKELGATLVLSNTYHLYLRPGDDVIAGLGGLHRFMRWPHPMLTDSGGFQVFSLAQT
                     RQIDDEGVTFKSHIDGSTHRFTPQRSIRIQENLGADIIMAFDECSDPNDHEYSRLAME
                     RTHRWAEICVKAKRRGDQALFGIVQGGVQPDLRAESAAFIASLDTPGIAIGGLSVGET
                     KEEMHNTLDVVTPLLPENKPRYLMGVGTPEDLINGVARGVDIFDCVLPTRLARHHSAF
                     APEGRLNLMNATFARDERPIDETCDCYTCKTFTRAYIRHLIVAKELLAGTLLSIHNLR
                     ALIRLMEEIRVYIAEGIFDEKAPLLLEQWSNNAKRLISE"
     gene            complement(1649..1975)
                     /locus_tag="VFQ23_09075"
     CDS             complement(1649..1975)
                     /locus_tag="VFQ23_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906783.1"
                     /translation="MIVTLFTTILVEGAIVSGYSLWHRKPVESILFTSVFANLITQYL
                     LWVVVNLFFQHYLTALLISEVLIWMIESLLLYRFAANRLGLQEAVLLSLFMNSASFGF
                     GLFLPV"
     gene            complement(1972..2499)
                     /locus_tag="VFQ23_09080"
     CDS             complement(1972..2499)
                     /locus_tag="VFQ23_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906784.1"
                     /translation="MILKRTKLVLLFLLLLAFLPARVALADTGPKPSMDFQFKQEMTG
                     EPPLTITSGTLYECDQPDCSDAAPIEEVGPQGFRCEVNSCSALAYGFAPYHRIAIEFS
                     DGRTRQSNVFETAGFESRYMVTIRPDDLLVDARFSVGVFPRTAILLIACLCAIVGVGL
                     IVGLITFLMRRTNKS"
BASE COUNT          668 a          665 c          694 g          618 t
ORIGIN      
        1 tcattattct ccgatgttat ttgatgattt gccagccgtt taaatattgg aacctgcttt
       61 caaagctctt ggtcattcca aactgcccat aatagatgac cgtgtacatt aagctatccc
      121 ctataaaact ttctggaagg atacttcctt ttgctccttc cacatttgct attatacatt
      181 cgcgcctggc agatagatca atgatcaaga gctgacaaaa tttgggcgtg cccccctcat
      241 cactttcctt ccattcctga acgacaagga atcgtgattc tggagaccac aagcagaccc
      301 ttccaaagat ccggcccgca aacgaatgcg gcagtccatc aatagataaa gagtaatagc
      361 tatgatctgc ctgaacttga ccgacaggtg tcaacacagc tttgtgcttc ttgtttggag
      421 attgaaattc aattggtttt gacattagtg ctccgtattt tctacccggg atccattatt
      481 cacttatcaa ccgctttgca ttatttgacc actgctccag caacagggga gctttctcat
      541 caaagatacc ttctgcaata taaaccctga tttcctccat aagcctgatc agtgcgcgca
      601 gattatgaat ggagagcaga gttcccgcca gtaattcctt ggcaacgatc aagtgacgaa
      661 tgtatgcccg cgtgaatgtt ttgcaggtgt aacaatcaca cgtttcatcg atggggcgct
      721 cgtcacgggc gaaggtcgcg ttcatcaggt tgagacgccc ctcgggcgca aaggcggaat
      781 gatgccgtgc caggcgggtc ggtaacacac agtcaaagat atccacgccg cgcgccacac
      841 cgttgatcaa gtcttcgggg gtaccgactc ccatgaggta gcgcggtttg ttttcgggca
      901 ggagtggggt gaccacatcc agcgtattgt gcatctcctc ttttgtctca ccgacggaga
      961 gtccaccaat ggcgattccg ggcgtatcca gtgatgcgat aaatgcagca gattccgccc
     1021 ggaggtccgg ttgaacgccg ccctggacaa tgccaaatag tgcctggtcg ccgcgccgct
     1081 tggctttgac acaaatttcc gcccagcggt gagtccgttc catggcaagc ctgctgtatt
     1141 cgtgatcatt gggatcggag cactcgtcga atgccatgat gatgtctgcg ccgaggttct
     1201 cctgaattcg aatggagcgt tggggtgtaa agcggtgcgt cgagccgtcg atgtggcttt
     1261 tgaaggtcac gccttcatca tcgatctggc gggtctgcgc cagcgagaag acctggaagc
     1321 cgcccgaatc ggtgagcatg ggatgcggcc agcgcatgaa ccggtgcaag ccacccagcc
     1381 cggcaatgac atcatcgcca gggcgcaggt agaggtgata ggtgttggag agcacgagcg
     1441 tggcaccgag ttctttgacc tggacaggcg tgagagtctt gactgttgcc tgggtgccga
     1501 ccggtgcgaa taccggtgtg atgaggttgc cgtgaggtgt ggtgaaaatc cccgtacggg
     1561 cgcgcttgtt gcgggctgtg atggtgaatt tgaacatgga tggaattata cagtagcggc
     1621 gaccttacac catcgcatag atatagattc aaactggtaa aaacaagcca aaaccaaaac
     1681 ttgccgagtt catgaacagg cttaggagta cagcttcctg taagccgagc cggttcgcag
     1741 cgaaacggta taacaaaagg ctttcgatca tccaaataag aacttccgaa atcagtaagg
     1801 cggtcagata gtgttggaag aacagattta caaccaccca gagcaggtat tgggtgatca
     1861 ggtttgcgaa aacgcttgtg aagagaatgg actcgacggg tttcctgtgc cagagtgaat
     1921 acccgctgac aattgcccct tcgacgagaa tggtcgtaaa aagagtgacg atcaactctt
     1981 gttggtgcgg cgcatgagga aagtaatcag accgacaatg agaccaacac caactatcgc
     2041 acagagacag gcgatgagca ggatggctgt gcgcggaaag acacccacgc taaatcgagc
     2101 atcgaccagc agatcatccg ggcggatggt gaccatgtat ctcgactcga acccggccgt
     2161 ttcaaagaca ttgctctgac gtgtcctccc gtcggaaaat tcgatcgcga tccggtgata
     2221 aggagcaaaa ccataggcaa gagcactgca actgttaacc tcacaccgaa atccctgcgg
     2281 acccacttct tcaatcggcg cggcatcact gcaatcgggc tggtcacatt cgtataaagt
     2341 ccccgaggtg atcgtcagcg gcggctcccc ggtcatttcc tgcttgaact gaaagtccat
     2401 gctcggcttg ggaccggtat cggcaagcgc cacccgggca ggcagaaatg ccagcaaaag
     2461 gaggaacagc aaaaccaatt ttgtccgttt taggatcatc ttttactctt gaaatggatt
     2521 aagggttagg actgcggatc gtgatgggga acgataagaa aattataatc ggtaacataa
     2581 aaagaatttg aaggatccca ccccaactcc tgctttgatc catggcttca tgacttcctc
     2641 ctatg
//
LOCUS       DASTJP010000302         4062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_780735, whole genome shotgun sequence.
ACCESSION   DASTJP010000302 DASTJP010000000
VERSION     DASTJP010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780735"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFQ23_09085"
     CDS             <1..720
                     /locus_tag="VFQ23_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9906785.1"
                     /translation="LRDRGFDPDTIFHPEDVDRRARADYNAAAISACLRLWIESQGHG
                     GRIAEARVLEVAQEITATTGHPMPILGKQTLRDGKSGIPYDQPVTVGVMTILKLHHLV
                     EDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDD
                     VQGRVNTYEAIVKGEPIEEPSIPASFRVLVKELQSLGLAVEAINEGGDVVKFGKDEEK
                     QHPPKLNTGLLGIGDNLMTKQ"
     gene            892..1308
                     /gene="rpsL"
                     /locus_tag="VFQ23_09090"
     CDS             892..1308
                     /gene="rpsL"
                     /locus_tag="VFQ23_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET9906786.1"
                     /translation="MPTINQMVRRGRKNKKVKSKAPALQYTLNSYKQRRVRQDKGAPQ
                     KRGVCTVVRTMTPKKPNSALRKIARVRLTNGIEVTAYIPGEGHQLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGSLDTTGVANRKQGRSKYGAKRPK"
     gene            1327..1794
                     /gene="rpsG"
                     /locus_tag="VFQ23_09095"
     CDS             1327..1794
                     /gene="rpsG"
                     /locus_tag="VFQ23_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET9906787.1"
                     /translation="MRRAKPESREILPDIRYNSIHVQTMVQHVLKRGKKSVATGLIYK
                     AMDLIKDRMDKNPLDVFEGALKNVSPVMEVRPRRVGGATYQVPMEVSADRRTTLAIRW
                     ILSAARERAGKSFPDKLAGELIDAFNETGTAIRKRDETHKMAEANRAFSHYRV"
     gene            1829..3892
                     /gene="fusA"
                     /locus_tag="VFQ23_09100"
     CDS             1829..3892
                     /gene="fusA"
                     /locus_tag="VFQ23_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9906788.1"
                     /translation="MAREYPLEKYRNIGIIAHIDAGKTTTTERIMFYTGKTHRIGSVD
                     DGTTVTDWMVQERERGITIVSAAVSAEWKGYQFNLIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDAVQGVEPQSETVWRQADRYGVPRICFVNKMDRVGASYERTIESIKDRLGANPI
                     PMQLPIGFEATFRGVIDLLEQKAIFWEDDLGKEPKIGEIPDDLLEQVKVARAFMVEKI
                     AELDDDLTVKYLEAQEISIPELKAALRKAVIANKAAPVFCGSSLKNKGVQVLLDAVVD
                     FLPSPADIPPVNANDEDGEVIELPAEDGAPLSALVFKIVTDPYVGRLAYFRVYSGVVS
                     QGQMLNNSTKGRKERIGRLIRMHADRREDITEVRAGDIGAVLGLKETFTGDTLCDNKK
                     VVLESISFPEPVISIAIEPKSSADQEKMGEALRKLAEEDPTFRVRSDENTAQTIISGM
                     GELHLDILVDRMLREFRVQANVGKPRVAYRESITRPVAKVDHKYAKQSGGRGQYGHVV
                     ISLEPGERGSGIIFENKIVGGSIPKEYIPAVQKGVMEASESGVLAGYPVVDMKITLFD
                     GSFHEVDSSEMAFKMAASMAFKEGVQRGSPVLLEPIMKVEVIVPDDYLGDVIGQINSR
                     RGSIQGMEVRPGNAQAVRSMVPLGEMFGYATELRSATQGRGVFSMEFDHYAPVSQTAA
                     LEIMK"
     gene            3936..>4062
                     /locus_tag="VFQ23_09105"
     CDS             3936..>4062
                     /locus_tag="VFQ23_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9906789.1"
                     /translation="MAKAKYDRSKPHLNVGTMGHIDHGKTTLTAAITKVAGLLGQA"
BASE COUNT         1107 a          881 c         1076 g          998 t
ORIGIN      
        1 cttcgggatc gaggttttga cccggataca atttttcatc cagaagatgt tgaccggcga
       61 gcacgagcag attacaacgc ggccgccatc agtgcctgtc tccgcctgtg gattgaaagc
      121 caagggcatg gtggcaggat agctgaagca cgagttttgg aagtggctca ggaaatcact
      181 gctacaactg gtcatcccat gcctatcctt ggtaaacaaa ctctgcgaga tggcaagtct
      241 ggaatcccgt atgaccagcc cgttactgtg ggcgtaatga caatattgaa gttgcatcat
      301 ttagttgaag acaaagtaca cgcgcgttcc acaggaccct atagcctggt aactcagcag
      361 ccacttggtg gcaaggcaca atttggcggt cagcgatttg gcgaaatgga agtatgggca
      421 ctggaagcgt atggtgctgc ttatacttta caggaaatgc ttacagtgaa gtctgatgat
      481 gtacagggac gcgttaacac gtacgaagcg attgtaaagg gcgaacccat tgaagaacca
      541 agcattcctg cgtcattccg cgtactcgtt aaagagctgc aatcgctggg tttggctgtt
      601 gaagcgatca atgaaggcgg tgacgtggtt aagttcggta aagatgaaga aaaacaacat
      661 ccacccaaac ttaatacagg gttgctgggc attggcgata acctgatgac caagcagtaa
      721 tcttgacgat aatcaaagtg agtgataaaa tctcatttcg ttgccaatag aaaaagcggc
      781 gatatctccc gctcaaaggc atggcactac aaatgaggcc atcaatatcc ttgtgatggc
      841 ctcatttctt gtgaaaaaac aaagtataga aagaaaaatt ggaggctgaa ggtgccgact
      901 atcaatcaaa tggtccgcag aggacgcaag aataagaaag taaagagcaa ggctccagcg
      961 ttgcaatata cgttgaactc atacaagcag cgtcgtgtac gccaggataa gggtgcaccc
     1021 caaaagcgtg gcgtgtgtac tgttgtccgc acgatgactc ctaaaaagcc aaactccgct
     1081 ttgagaaaga ttgcccgcgt ccgtttgaca aatggcatcg aagtcacagc ttacattcca
     1141 ggtgaaggtc accagcttca ggaacactcc gtggtattgg tccggggtgg tcgtgtgaaa
     1201 gatctgccag gtgttcgtta tcacattgta cgcggctccc tggatacaac cggcgtcgcg
     1261 aatcgcaaac agggtcgctc caaatatggc gcgaagcgcc ctaaataatt gatggagtaa
     1321 tagacgatgc gtagagcaaa acctgaaagt cgggaaattc ttccggacat ccgatataac
     1381 agtattcatg tgcagaccat ggttcagcat gtgctcaagc gcggcaagaa gagtgtcgcc
     1441 actggtctca tctacaaggc catggatctt attaaagatc gtatggacaa aaatcctctg
     1501 gatgtttttg agggtgccct taaaaacgtt tccccggtta tggaagttcg cccccgccgt
     1561 gttggtggcg caacctacca ggtgcctatg gaagtatcag cggaccgccg cacaacactg
     1621 gcgatccgct ggattttgtc tgcggctcga gaacgcgcag gaaaatcctt tcccgataaa
     1681 ttggcaggag aattgatcga cgccttcaat gaaactggca ctgcaattcg taaacgggac
     1741 gaaacccata agatggctga agccaatcgt gcattttcac attatcgtgt ttaattttat
     1801 aaaaaaagtg taaaggttat ttagaagaat ggctcgtgag tatccgcttg agaaatatag
     1861 aaatataggg attattgccc acattgacgc tggtaaaaca acgactaccg agcggattat
     1921 gttctatacc ggcaagacgc atcgtatcgg ttcagtagat gacggcacga ccgtcacgga
     1981 ctggatggta caggaacgtg aacgcggtat taccattgta tctgcggctg tatcggctga
     2041 atggaagggt tatcaattta acctaatcga tacacccggt catattgact ttacagctga
     2101 agttcaacgc tcccttcgtg tattggatgg tggcgttgtt gtctttgatg cagttcaagg
     2161 cgtggagcct cagagtgaaa cggtttggcg tcaggctgat cgttacgggg ttccacgtat
     2221 ttgttttgta aataagatgg atcgagtggg cgcctcctat gaacgcacca tcgaatccat
     2281 taaggatcga ctgggtgcaa acccgattcc gatgcaactc ccaatagggt ttgaagctac
     2341 gttccgtggc gttattgatt tgcttgaaca aaaagcaatc ttttgggaag atgatcttgg
     2401 taaagaacca aagatcggtg aaatccctga tgatctactg gaacaagtca aagttgcccg
     2461 cgcattcatg gttgagaaga ttgccgaact ggatgatgat ttaaccgtca agtatctgga
     2521 agctcaggaa atcagtattc ctgaactcaa ggcggcattg cgcaaagctg tgatcgcaaa
     2581 taaagctgcg ccagtcttct gtggatcttc attgaagaac aagggtgtcc aggttttgct
     2641 tgatgcagtt gtggattttc tcccttcccc ggctgatatt ccacctgtaa atgcaaatga
     2701 cgaggacggg gaagtcattg agttgccggc agaggatggt gcgcccctta gcgctctcgt
     2761 tttcaagatc gttaccgatc cgtatgtcgg ccgtttagcc tattttcgag tttattcagg
     2821 agttgtcagc caggggcaaa tgttgaacaa ttccacaaag ggtcggaagg aacgcattgg
     2881 tcgcttgatt cgaatgcatg cggatcgccg tgaagatatc acggaagtac gcgccggtga
     2941 tatcggtgcc gttctcggct taaaagaaac gtttactggc gatacgttat gcgataataa
     3001 gaaagttgtt ctagaaagca tatccttccc tgaaccagtg atttccatcg ctatcgaacc
     3061 gaagagcagt gccgatcagg aaaagatggg tgaagcactt cgcaaacttg cagaagaaga
     3121 tcctacgttc cgcgtccgct cggatgaaaa tactgcccag acaatcattt ctggtatggg
     3181 cgagctacac cttgatattt tggttgaccg catgttgcgt gagtttcgcg ttcaggcaaa
     3241 tgttggtaag ccgcgcgtag cctatcgtga gtcgatcaca cgccccgtag caaaagtcga
     3301 tcataagtac gccaaacaat cgggcggtcg tggtcaatat ggacacgttg tgatttcact
     3361 tgagccgggt gaacgcggca gtggcattat ttttgaaaac aaaattgttg gcggttccat
     3421 tcccaaggaa tacattcctg ccgttcaaaa aggtgtcatg gaagcttcag agagtggtgt
     3481 gctggctggg tatcctgtcg ttgatatgaa aatcaccttg ttcgatggat ccttccatga
     3541 ggttgactcc agcgaaatgg ctttcaagat ggcagcctct atggctttca aagagggtgt
     3601 tcaaagggga tcgcccgtgc tactcgaacc gattatgaaa gttgaagtca tcgtcccgga
     3661 tgattatttg ggagatgtaa tcggtcagat caatagtcga cgcggcagca ttcaaggcat
     3721 ggaagttcgc ccgggcaacg cccaggctgt tcgttccatg gttccattgg gtgaaatgtt
     3781 tggttatgca acagaattgc gttcagccac acaaggtcgc ggcgtattta gcatggaatt
     3841 tgatcactat gcaccagttt ctcaaacggc tgcattagaa ataatgaaat aatcataata
     3901 ttcttggtat ttatcaattg gaaggagagt aatcaatggc gaaggcgaaa tatgacagga
     3961 gcaagccaca tctgaacgtg gggacgatgg gccacatcga ccacgggaag acgacgctga
     4021 cggcagcgat cacgaaggta gcgggattgc tgggacaggc gg
//
LOCUS       DASTJP010000303         4126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1334479, whole genome shotgun sequence.
ACCESSION   DASTJP010000303 DASTJP010000000
VERSION     DASTJP010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334479"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VFQ23_09110"
     CDS             complement(<1..806)
                     /locus_tag="VFQ23_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9906790.1"
                     /translation="MEYRHLGRTGIRVSELSLGSWITFKNQVDVKAAVEIMAAAYDAG
                     VNFFDNAESYAKGQSEQVMGSALKQLKWRRGSYLVTTKLYWGMYDGINEKNTLNRKRL
                     IEGINGSLERLQLDYVDVLYCHRPDKSTPIEETVWAMHNIIEWGKAFYWGTSQWAADE
                     IVAAIEIADKHHLHKPVTEQTEYNLLERRRFEKEYARLFKDYGYGSTTWSPLATGILT
                     GKYLEGIPDESRAALKGFDWLHEAVTDKEKMDKVRALQPIAEELGCTLSQL"
     gene            902..1777
                     /locus_tag="VFQ23_09115"
     CDS             902..1777
                     /locus_tag="VFQ23_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET9906791.1"
                     /translation="MQVLDYIAAGSVHEVIGLLTANNGKARVLSGGTDLIVQLRENRR
                     RAELLIDLKKIDELTSIRYDPQNGLRIGAAASCSAICQNEAVIANFPGLVDGIRLIGG
                     VQIQSRASIGGNLCNASPAADSIPALIVHGARCLIVNNNGTHEIPVEQFCVAPGRNIL
                     RSGEFLLAVVVPVIPENFGAHYLRFIPRNEMDIAVVGAGASVTLDQAKEKVVSARVAL
                     GAVAPTPLLVEEAGSFLAGKEITTENIQEAAKIAQAAARPISDLRGTAEHRKHLCAVL
                     TRRALEGAIERARVA"
     gene            1774..2256
                     /locus_tag="VFQ23_09120"
     CDS             1774..2256
                     /locus_tag="VFQ23_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9906792.1"
                     /translation="MSKKVQVQASINGEPVEFLCEPRQSLLEVLRETLGLTGAKEGCN
                     NGNCGACNVILDGKLVNSCLVLAVEIHGQNVTTIEGIATAQGLHPLQEKFISHAALQC
                     GFCTPGFIVASKALLDRKPNPSESEVREWLAGNLCRCTGYDRIVRAVLDAAQVQEKTL
                     "
     gene            2253..>4126
                     /locus_tag="VFQ23_09125"
     CDS             2253..>4126
                     /locus_tag="VFQ23_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET9906793.1"
                     /translation="MTINELVDENKTTYQVIGKNPTRHDGTDKVTGRALYGADIRLPG
                     MYYGAMLRSPHAHARILSIDTSEAESYPGVKAVVTAKDLPDVENKIADLGEGTINLRH
                     QSNNVLAKDKVLYFGHAIAAVAATSTHAAEEAAALIKVEFEVLPPVLDVRKAMQKDSA
                     ILLEDMRTDELGKKGDAPSNVASHSQEKAGDVEKGFEDAAVIVEREFFTSMVHQGYIE
                     PQNATAQYNPDGQITIWCSTQGAFGVREQTAEILDIPISRIRVIPMEIGGGFGGKVGV
                     YLEPVVVLLSRKSGYHPVKMTMSRSDVLAATGPTSGSYIKIKMGADGNGKLIAAQATL
                     MYEAGAYPGSPVGGAMAVMFAPYRIENVLVDGYDVLVNKPRTAAYRAPGGSNAALASE
                     TVIDELCEKLGMDPIEFRKLNGVKEGDRRVDGPVYKRIGFLETLEAAQNSPHYCSPLE
                     CPHRGRGVASGFWFNYGGKSSASASINKDGTISLITGSVDIGGTRTSIAMQLAEGLGL
                     PVESIRPGVADTDSVGFTEGTYGSRTTFATGWAVYEISNQMKEELLKRAAIIWETEPD
                     TIRFENGTFLFEAQRMTFAELASRLDDTGGPVVASAAVRPEQSGPAFATHIVDVEVDP
                     "
BASE COUNT         1009 a          975 c         1139 g         1003 t
ORIGIN      
        1 agttgtgaaa gagtgcaacc cagttcttcg gcgatcggct gcagcgctcg gactttgtcc
       61 attttttctt tgtcggtcac tgcttcatga agccagtcga atcccttcag ggcagcccgg
      121 ctctcgtctg gaattccttc cagatatttt cctgtcagaa ttcccgtcgc cagcgggctc
      181 cacgttgttg agccgtaccc atagtccttg aacaaacgtg catactcttt ttcgaagcgc
      241 cttcgctcta acaaattgta ttcggtctgt tccgtgacag gtttgtgtaa gtggtgttta
      301 tctgcaattt caatagcagc cacaatttca tccgcggccc actgcgaggt tccccagtaa
      361 aacgccttgc cccactcgat aatgttgtgc atcgcccaaa ctgtttcttc gatgggggtt
      421 gacttatcag gacgatgaca atagagcaca tcgacatagt ccagttgtaa acgttcgaga
      481 gagccgttga tgccttcgat gagtctcttg cgatttagcg tgtttttctc attgattcca
      541 tcatacatgc cccagtaaag tttggtcgtc accaagtaac taccgcgcct ccactttaat
      601 tgcttcaacg cggatcccat cacttgttcc gattgtccct tcgcatacga ttcagcgtta
      661 tcgaagaaat tcacgcctgc gtcataagcg gccgccatga tctctaccgc agctttgacg
      721 tccacctggt ttttaaatgt aatccacgaa cccaaagaaa gttcgctgac gcgaatcccc
      781 gtgcgaccaa gatgccgata ttccatactg cctccataat aaagttttgt gtaagcttgt
      841 atgaattata atccccgaga cttttattgg tgcagcgtag attccatgtt cggaggtgtt
      901 catgcaggtc ctcgattata tagcagcagg gagtgttcac gaggttattg gcctattgac
      961 agccaacaat ggaaaggcac gtgttttaag tgggggcacc gatctgatcg tgcaattaag
     1021 ggagaatcgt cgtagggcgg aactgttgat tgatctcaag aaaattgatg agcttacctc
     1081 cattcgctac gaccctcaga atggcttacg gattggggct gcggcttctt gttcggcgat
     1141 ctgtcagaat gaggcagtca tagcaaattt tccaggtttg gtggatggaa ttcgtttgat
     1201 tggcggagta cagatccaat ctcgagccag catcggtggc aatttatgta acgcatcacc
     1261 ggcagcggat tctattccgg ccttgatcgt ccatggcgcc aggtgtttga tcgtgaataa
     1321 caacggaact catgaaatac cagtggaaca attctgtgtt gcaccaggca ggaacatttt
     1381 gcgaagtgga gaattcctgc tggctgttgt agtacctgta attccggaaa acttcggcgc
     1441 acattatctg cgcttcattc ctcgcaatga gatggacatt gcggttgtgg gcgcgggagc
     1501 gtccgtaacg cttgaccagg cgaaggagaa agtagtatca gccagggttg cgcttggagc
     1561 cgtcgctcca actccattgc ttgtagagga agccggatca tttttggcgg gcaaggaaat
     1621 cacaacggaa aatatccagg aggcggcgaa gatcgcacaa gctgcagcgc gcccgatttc
     1681 agatttgcgc gggacggccg aacatcgaaa acatctttgt gcagttctca cgcgccgcgc
     1741 gctcgaggga gccattgaaa gagcgagggt agcatgagca agaaggttca agtgcaggca
     1801 tcgatcaatg gcgaacctgt tgagttcttg tgtgagcccc gtcagagtct gctggaagta
     1861 ttgcgcgaaa ctctcgggtt gaccggtgcc aaggaaggct gcaacaacgg caattgcggc
     1921 gcgtgcaatg tgattttgga tggcaagctg gtgaactcct gtttggtctt ggcagtggag
     1981 atccacggcc agaatgtgac aacgattgaa ggaatcgcaa cagcgcaggg attgcatcct
     2041 cttcaggaaa aattcatctc gcatgcggct cttcaatgtg gattttgcac tccagggttc
     2101 atcgtcgctt cgaaggcctt gttggatcga aaacctaacc caagtgagag tgaagtgcga
     2161 gaatggctcg caggtaatct ctgtcgctgc actggttatg atcgaatcgt ccgtgcagtg
     2221 ttggatgcag ctcaagtcca ggagaagaca ctatgaccat aaacgaattg gtcgatgaga
     2281 acaaaactac ctatcaggtg atcggaaaga atccgacacg tcacgatggg accgacaaag
     2341 tcactgggcg cgccctctat ggtgctgata ttcggctacc cggaatgtat tatggcgcga
     2401 tgttgagaag cccacatgca cacgctcgga ttttatcgat cgatacaagc gaagccgaat
     2461 cttatcccgg agtaaaggca gtcgttaccg ccaaagacct tcccgatgtg gaaaataaaa
     2521 tcgcagacct gggcgaaggg acgattaact tgcgccacca aagcaacaat gtcctcgcca
     2581 aagacaaagt gctgtatttc ggccatgcca ttgctgccgt agctgccacc agtacgcatg
     2641 cagcggaaga agccgcggcg ttgattaagg tggagtttga agttttgccg ccagtgctgg
     2701 atgtaaggaa agcaatgcag aaagattcag ccatcttgct tgaggatatg cggacggacg
     2761 agctaggaaa gaaaggcgat gctcccagta atgttgcctc tcactctcag gaaaaagctg
     2821 gcgatgtgga aaagggattt gaggatgccg ctgtaattgt ggagcgggaa ttctttactt
     2881 ccatggtgca ccagggatac atcgaaccac aaaatgcaac cgcgcaatac aatccggatg
     2941 ggcaaattac aatctggtgc agcacacagg gcgccttcgg tgtgcgggaa caaactgcgg
     3001 agattcttga tatcccgatc tcaagaatcc gcgtgattcc gatggaaatc ggaggcgggt
     3061 tcgggggaaa ggtcggggtc tacctggagc cggttgttgt cttgctgtcg cgcaagagtg
     3121 gatatcaccc ggtcaagatg acgatgagcc gcagtgacgt gcttgcagcg acgggtccta
     3181 catccggttc gtatatcaaa ataaaaatgg gcgctgatgg aaatgggaag ctcattgcgg
     3241 cgcaggcaac gctgatgtat gaagcgggtg catacccggg ctcgccggtt ggtggtgcaa
     3301 tggctgtaat gtttgcaccc tatcgaattg aaaatgtact ggtggatgga tacgatgtac
     3361 tggtcaacaa gccgcgcaca gctgcatatc gtgcgcccgg aggttcgaac gcggcgctgg
     3421 cgtctgaaac agtcattgac gaattgtgtg aaaagcttgg aatggatcct atcgaattcc
     3481 gcaagttgaa tggtgttaaa gaaggggatc gccgcgtgga cggtcccgtc tataagcgca
     3541 tcggatttct ggagacgcta gaagccgcgc agaattcccc acattattgc tcgccgctag
     3601 aatgtccgca tcgaggacgt ggcgttgcat ccggcttttg gtttaattac ggtggaaagt
     3661 ccagcgcgtc ggcgagtatc aacaaggatg ggacaatcag cctcataacg ggttcagtgg
     3721 atattggggg tacgcgtacc tcgattgcaa tgcaactggc ggagggactg gggcttccgg
     3781 ttgaatcgat tcgccctgga gtcgcagata cagattcggt gggattcact gaaggtacct
     3841 acggcagccg cacgacgttt gctaccggtt gggcagtcta tgaaatcagc aaccaaatga
     3901 aagaagaact attaaaacgt gccgcaatta tttgggaaac cgaaccggat acgatccgtt
     3961 ttgagaatgg tacttttttg tttgaagctc aacggatgac ctttgcagag ctcgcctcac
     4021 gtctggacga taccggtggt cctgtagttg ccagtgccgc cgttcgacca gagcaatcag
     4081 gacccgcttt tgcgactcac atcgttgatg tcgaagtgga tccaga
//
LOCUS       DASTJP010000304         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_975303, whole genome shotgun sequence.
ACCESSION   DASTJP010000304 DASTJP010000000
VERSION     DASTJP010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975303"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFQ23_09130"
     CDS             complement(<1..368)
                     /locus_tag="VFQ23_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9906794.1"
                     /translation="MKELKDYRKSLMDKLVNTARDFHEASLAIEDPYKPLEEGGWNIH
                     QMAVHTRDVDKLVYSLRARRTLEENNPEFQSFDGDTYMAEHYNAKEPLNEILNELVEN
                     IESLINMLRTLPEEAWARRSR"
     gene            complement(425..1132)
                     /locus_tag="VFQ23_09135"
     CDS             complement(425..1132)
                     /locus_tag="VFQ23_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906795.1"
                     /translation="MSKRFLILSILVVIVIAAAGTAYYTYQPNIARLDKFRQWRQDPA
                     SHSDWALVAGSQCGSAPFLFPTDGFAGFLWGDSFGLRHTHQGIDVFAGTDVGITRVVS
                     AYPGYLTRSPEWKSAVIVRVPDDPLQPGRQIWLYYTHMADENGNSFIASEFPAGTVEK
                     FIEAGTFLGYQGNYSGDPSNPVGVHLHFSIVEDDGTGTFKNELDIKNTLDPSPYLGLP
                     LNGYENRDLVPVCQSDS"
     regulatory      1244..1338
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1443..>2408
                     /locus_tag="VFQ23_09140"
     CDS             1443..>2408
                     /locus_tag="VFQ23_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9906796.1"
                     /translation="MHRKLFSVFSLVLVLAVAFSAVAPVAAQPSAKAKGGDLTESPNG
                     IYIVQMLDKPVVAYDGGINGLRPTKPGNGQKINPLSNDVVRYVAYLNGRHNGALNSVG
                     GEKVYSYYYSYNGFAARMSLAQAQKLANVDGVLVVNPDQIYTTDTSSTPSFLGLDAPG
                     GLWSQLGGPAGGKSVAGAGEDIIIGIIDSGIWPESLSFSDRDAEGKLVYQQIPGWHGK
                     CTPGEEFNASLCGQKLIGAQHFNAAWGGDAALEAQRPWEFMSVRDYNGHGTHTASTSG
                     GNYGVATTGPSTAIGSSISGIAPRARIAMYKALWSTQDASTASGNG"
BASE COUNT          631 a          553 c          580 g          644 t
ORIGIN      
        1 cgggaacgcc gcgcccatgc ttcttcgggc aacgttcgta gcatgtttat taacgactca
       61 atgttttcaa caagctcatt caatatttca tttaatggct ctttggcatt gtagtgctcc
      121 gccatatagg tgtcgccatc aaagctttga aactcgggat tgttctcctc aagggtccgt
      181 ctggcgcgca gactgtaaac taatttatcg acgtcgcgcg tatgaaccgc catttgatga
      241 atattccagc ctccttcttc taaaggttta tacgggtctt ctattgcaag actcgcctca
      301 tgaaaatctc tggctgtatt tactaattta tccatcaggc ttttgcgata atctttcaac
      361 tctttcatcg cacctccatg tagaaactat gcagagtaag aatgctcaga gccattttat
      421 ctctctaact atctgattga caaacaggca caaggtcacg gttttcatat ccattcaagg
      481 gtaaccccaa atatggcgaa ggatcaaggg tgtttttgat atccagctca ttcttgaacg
      541 taccagtacc gtcatcttca acaattgaaa aatgcagatg aacaccaacc gggttgctag
      601 gatctcctga ataatttccc tgatacccca ggaaagtacc tgcctcgata aacttttcaa
      661 cggtaccagc cggaaattca gaagcaataa atgaattacc gttttcatct gccatgtgtg
      721 tgtagtagag ccagatttgt cttcctggtt gaagggggtc gtctgggact cgaacaataa
      781 ctgcggattt ccattctggt gagcgcgtga ggtagccagg ataggcagac acgacacgtg
      841 taattcctac atctgtacct gcaaacacat caatgccctg atgcgtatga cgcagtccga
      901 aggaatctcc ccaaagaaaa cccgcaaagc catctgttgg aaacagaaat ggggcagagc
      961 cgcattgaga gccagcgacc agtgcccaat ccgagtgaga tgcggggtct tgtctccact
     1021 gacgaaactt atccaggcgt gcgatgttgg gttgatacgt atagtaagcc gtccccgcgg
     1081 ctgcaatcac gattacaact aggattgaaa gtatcagaaa tcgtttagac atagggcagg
     1141 attataacgg tggaaaacaa ttcaggcact tgttatatta ccttttatta gtaaaagtta
     1201 cacctcttga atttcttcac taattaaagt aagattaaat ttagaatcga aaaggcaaac
     1261 ccgccgcgag acggggacgc aaagtcatgg atctgccaaa ctgatgcgta caggttgaca
     1321 gaccgccaga ccgccgaact tttttgttcg gtggttttct attaaggagg ttttcggcaa
     1381 aatatcatta agctgcacta aaagtttcaa atcagattct ttctattgag gagacaaaga
     1441 tcatgcatcg aaagcttttt agtgtttttt cgcttgtgct tgtgctggca gtggcatttt
     1501 cggcggtagc cccagttgca gcccaaccaa gcgctaaggc aaagggtgga gatcttacag
     1561 agagtcctaa cggaatatac attgttcaaa tgctggataa accagtagtc gcttatgatg
     1621 ggggcatcaa tggtttgaga cctaccaagc ctggaaacgg acagaaaatc aaccccctca
     1681 gcaatgatgt ggttagatat gtggcatatc ttaacggcag gcataatggt gctcttaaca
     1741 gtgtgggcgg agaaaaagtt tatagttact attacagcta caacggtttt gctgcaagga
     1801 tgagccttgc tcaggctcag aagctcgcca atgttgatgg cgttttggtt gtcaaccctg
     1861 atcagatcta tactacagat acttcctcca cgccaagttt cttaggcctt gatgcacctg
     1921 gtggcttatg gtctcaattg ggtggtccag ccggcggtaa gagtgttgcc ggagcaggcg
     1981 aagatattat catcggcatc atcgactctg gcatctggcc cgagagcctg agcttcagcg
     2041 atcgagatgc tgaggggaag cttgtctacc agcagattcc tggctggcat ggcaaatgta
     2101 cgcctggcga ggaattcaat gcctccttgt gcggtcagaa actaattggt gcgcagcact
     2161 tcaatgcggc gtggggtggc gatgcggcct tggaagccca acgcccttgg gaatttatgt
     2221 ctgttcgtga ttacaatgga catggcacac acacagcctc cacgtctggc ggtaactatg
     2281 gcgtggcaac tactggtcct tcgactgcga ttggttcctc tatcagtggt attgctcccc
     2341 gcgcacgaat tgcaatgtat aaggcattgt ggtctacgca ggatgcctct acagccagtg
     2401 gaaatggt
//
LOCUS       DASTJP010000305         5776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_850277, whole genome shotgun sequence.
ACCESSION   DASTJP010000305 DASTJP010000000
VERSION     DASTJP010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850277"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /gene="glpK"
                     /locus_tag="VFQ23_09145"
     CDS             complement(<1..1291)
                     /gene="glpK"
                     /locus_tag="VFQ23_09145"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET9906797.1"
                     /translation="MPKYVAAIDQGTTSTRFMIFDHSGNVIAVDQKEHQQIFPKPGWV
                     EHDPLEIWARTQEVINGALAKLSGFQPTDIASIGITNQRETTVVWDKNTGKPIYNAIV
                     WQDTRTDVIVNALAQDGGQDRFRDKTGLPLATYFSGPKITWILDHAPEARTKAENEEL
                     LFGNIDTWLIWNLTGAHITDVTNASRTLLMNLSTLDWDDEILKALDIPRAMLPEIKSS
                     SEIYGSVGKSSCPSLQNVPVSGDLGDQHAALFGQTCFNAGEAKNTYGTGCFMLMNTGE
                     SPVPSQAGLLTTLGYKIGDQKAVYALEGSIAITGALIQWLRDNLGLIQSSAEVEELAR
                     SVEDNGGIYFVPAFSGLFAPYWKSDARGAIVGMTRYVNKGHIARAALEATAYQTREVL
                     DAMEADSGVTLTALKVDGGMVFNELLMQFQSDILNVPV"
     gene            complement(1327..2037)
                     /locus_tag="VFQ23_09150"
     CDS             complement(1327..2037)
                     /locus_tag="VFQ23_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET9906798.1"
                     /translation="MFNSKVFVAEFIGTFALIFIGAGAAVTGLGGLLGAALAHGFVVA
                     VFAYAYGPISGSHINPAVTFGMALNNTIRWAEAVVYWIAQFAGAILAAYLLTVIVGSV
                     NLGGIETGATRGTLTEPAPYIALLVEIILTFFLVNTILHTDVEGNGGALSGLAIGLTL
                     VFSILAGGPMTGASLNPARTFGPAIFTGSASDPMTYLIYFAGPLLGAALAVGVFRFLR
                     SGAVPSHSEPASKVARRK"
     gene            complement(2092..2997)
                     /gene="fmt"
                     /locus_tag="VFQ23_09155"
     CDS             complement(2092..2997)
                     /gene="fmt"
                     /locus_tag="VFQ23_09155"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET9906799.1"
                     /translation="MTSVVFMGSPHFALPGLRELAEKYHVVGVVTQPDRASGRGREVK
                     MPPVKTLALELGIPVMQPEKLRAPDAMEKLREWNPELIVVAAFGQILKKDVLDLPRFG
                     CINVHASLLPRWRGAAPINAAILHGDEETGVTIMQMDVGLDTGPMLAKRSIRLTPDDT
                     AGSVTESLSHLGADLLIETLPDYLAGKIIAQPQPQEGGTYAPMLKKEEGKLDFTRDVH
                     ELERQVRAFNPWPGTFMDFDGTSLKIHKAHIEEEKSTEGQRLVYQDQPAVGARGGLLV
                     LDEVQPAGKKSMSGKSFLAGARRWV"
     gene            3153..3488
                     /locus_tag="VFQ23_09160"
     CDS             3153..3488
                     /locus_tag="VFQ23_09160"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET9906800.1"
                     /translation="MSQEPMMEATSDDKLWALLAYALSPLVPVIILLMEDKKNRPFIR
                     AHNAQALVWGLVNLIGGTILSGVLFFCFGLPSLLIWAVGVYWGIQAYNGKLVEIPVIS
                     NLVRNQGWA"
     gene            complement(3552..4607)
                     /locus_tag="VFQ23_09165"
     CDS             complement(3552..4607)
                     /locus_tag="VFQ23_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906801.1"
                     /translation="MWLIITILFIVGCGLFRPSATPTPDLLGTLQASTPSSLSSPAPG
                     DTVTTPDFSFPTILPTLPSEGSSIPAPSAADQLTGQIVFTCQIFKVTAIDQICVMNAD
                     GSNIRRLTTNNNVRHFYPSLSPDGQSVLYASFREQNVYEIYRIALSNGNVDQLTDRLG
                     ILTAPEVSPDGSSITFARATGDPGSQPQVMTMNSNGGEARSLSQVLGWDPTWSPDGSQ
                     ILYASSSQGKVQLFVVNRDGNGLRQVSNLPAIRGRSDWSADGQWIVTYSGPSWNRELY
                     IMNADGSNARQLTPTGGNSQGPSFSPDGKWVVFTAYFDKYGDDHGCEIYVIRIDGTDL
                     RRLTSNDYCDYQPRWGP"
     gene            complement(4676..5144)
                     /gene="rnpB"
                     /locus_tag="VFQ23_09170"
     ncRNA           complement(4676..5144)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFQ23_09170"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5429..>5776
                     /gene="coaE"
                     /locus_tag="VFQ23_09175"
     CDS             5429..>5776
                     /gene="coaE"
                     /locus_tag="VFQ23_09175"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.4"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET9906802.1"
                     /translation="MSILPGKYVIGLTGNIATGKSVVRRMLEHLGAYTIDADGLAHRV
                     IAKGAPGYQPVLDRFGTWLLESDGQINRNKLGRVVFADPEALAQLESIVHPYVSQAVD
                     LLARRATQPVVVIE"
BASE COUNT         1423 a         1515 c         1395 g         1443 t
ORIGIN      
        1 caactggtac attcaaaata tcagattgga attgcatcag caattcattg aataccatgc
       61 ctccatcgac tttgagcgca gtcaacgtta cacccgaatc cgcttccatc gcatccaata
      121 cttcgcgcgt ctgatacgcg gtcgcttcga gcgcggcgcg ggcgatatgc cctttgttga
      181 cgtaacgcgt cattccgaca atcgcaccac gcgcgtcaga cttccaatag ggggcgaaca
      241 atcccgaaaa cgcggggacg aaatatatac caccattatc ttcaacggat cttgctaact
      301 cttccacttc agccgaggat tggatcaatc ctaaattatc tctcagccac tgaatcaacg
      361 caccggtgat ggcaatcgag ccttccagcg cgtaaacagc tttctgatct ccgattttgt
      421 atcccaacgt cgtgagcaat ccagcctgcg atggcacagg agattcaccg gtgttcatca
      481 acatgaaaca tcccgtaccg tatgtattct tggcctcacc tgcgttgaag caggtttgtc
      541 cgaacaaagc agcatgttgg tcacccaggt caccggaaac aggcacgttc tgtagggaag
      601 ggcagctgct cttccctaca gaaccataaa tttcagaaga tgatttgatc tcaggcaaca
      661 tcgcacgcgg aatatcaaga gctttcagga tttcatcatc ccaatccagt gtggataaat
      721 tcatcagcag ggtgcgtgaa gcattggtca catccgtgat gtgagctcct gtcagattcc
      781 agattagcca cgtatcaata ttgccaaata atagttcttc attttcggct ttcgtacgag
      841 cttcaggagc atggtcaaga atccatgtga tttttggacc cgagaaatac gtcgcgagag
      901 gtaaaccagt tttgtctctg aatcgatcct gcccaccgtc ctgtgccagt gcattgacga
      961 tgacatccgt acgcgtatcc tgccagacaa tggcattgta gattggcttc cccgtgttct
     1021 tatcccagac gacggttgtc tcacgctgat tggtaattcc aatcgaggca atatctgtgg
     1081 gctgaaaccc acttaatttt gccaacgccc catttattac ttcctgcgtg cgagcccaaa
     1141 tttcgagagg gtcatgctcc acccaaccgg gctttggaaa aatttgctgg tgctcctttt
     1201 ggtcaaccgc aatcacattt ccactgtgat caaaaatcat aaaacgggta ctggttgtgc
     1261 cctggtcaat cgccgcgaca tattttggca ttcttcacct ctggaatggc gtaggagtaa
     1321 atcagcttat ttacggcgcg ctactttact ggccggttca gaatgcgaag gaactgcacc
     1381 ggatctcaaa aacctgaaga cacccaccgc caaggccgca cccaggagcg gacccgcaaa
     1441 atagatcaga taggtcatag ggtctgaagc gcttccggta aagatagcag gaccaaacgt
     1501 tcgcgccggg ttaagcgagg caccggtcat cgggccaccg gcaaggattg agaataccag
     1561 agtcaaccca atcgcgagac cagataaggc accaccgttt ccttccacat ctgtatgcaa
     1621 aattgtgtta accaggaaga acgtcaggat gatttccaca agcagagcga tatatggcgc
     1681 aggttcggtt aacgtgccgc gcgttgctcc ggtttcaatc ccacccaaat tcacactacc
     1741 cacaatgaca gtaagcaaat atgcagcaag gatcgcaccg gcgaattgag cgatccaata
     1801 aacaacggcc tccgcccacc ggatcgtatt attcaaggcc atgccaaatg tcaccgcggg
     1861 gttaatatgt gaccccgaaa tgggtccata ggcgtacgca aaaactgcaa ccacaaatcc
     1921 atgagccaac gcagccccaa gcaaaccacc taaaccggtc acagccgcac ctgcaccaat
     1981 aaatatcagc gcgaacgtac caatgaactc cgccacgaaa acctttgaat tgaacatact
     2041 gcctcctgat tgaaatgtca tgcgttgatg aacccgtatg ctcttacata gttacaccca
     2101 gcgacgcgcg ccagctaaaa atgattttcc actcatggat ttcttccctg ccggctgcac
     2161 ttcgtcgaga accagcagcc cgcctcgcgc gccgactgcg ggttggtcct gataaaccaa
     2221 cctctgccct tctgttgact tttcttcttc gatatgtgcc ttatgaattt tcaatgatgt
     2281 accatcgaaa tccataaacg tcccgggcca tgggttgaat gctcgcacct gtcgttctaa
     2341 ttcatgcaca tcgcgcgtga aatccagttt gccttcttcc ttcttgagca tcggtgcata
     2401 ggtgccgccc tcttgaggct gaggctgagc aatgattttg ccagctaaat aatctgggag
     2461 ggtctcgatt agcaaatctg cgccgagatg agataaactc tccgtaacgg aaccggctgt
     2521 atcatccggg gtgaggcgaa tcgagcgttt cgccagcatg ggaccggtat cgagtccgac
     2581 atccatttgc atgatggtca caccggtctc ttcatcacca tgtaaaatcg cagcattgat
     2641 cggcgccgcg ccgcgccagc gcggcaaaag cgatgcatgg acattgatgc acccaaagcg
     2701 cggcaaatct aagacgtcct tcttgagaat ctgaccaaac gccgcgacaa cgataagctc
     2761 tggattccac tcgcgcaatt tttccatggc atcgggtgcg cgcaactttt cgggttgcat
     2821 aaccggaatg ccaagttcga gcgccaatgt ttttaccgga ggcattttta cctctcgccc
     2881 acgaccagaa gcacgatctg gctgtgtcac caccccaacc acgtgatact tttcggccag
     2941 ttcgcgcaaa cctggcaatg caaaatgggg tgaacccatg aaaacgacgc tggtcatatg
     3001 gtcattttac cagcagggaa attgtttcct gcaagttgaa gattctcttt ttcatcatta
     3061 ttccaggttt gtttggggaa atcgaattgc attctatatt gtttggttgt aaaataagta
     3121 aaacctaatc ccagatcatt tcggaggaac tcatgtctca agaacctatg atggaagcta
     3181 ccagtgatga caaattgtgg gctttgcttg cctatgcgct ttcgcccctc gtcccagtca
     3241 tcattctatt gatggaggat aagaaaaatc gtcccttcat ccgtgcacac aatgcacagg
     3301 cattggtctg ggggctcgta aaccttatcg gcggaacaat cttaagtggc gtgttgttct
     3361 tctgcttcgg attgccctcc ctgttaatct gggcagtcgg tgtgtattgg ggcattcaag
     3421 cctataacgg taaactggtt gaaattccgg taatttcaaa tctggtgaga aatcagggtt
     3481 gggcataaac ctccttttca gatgaaaaaa gctcacatcg ttgatgtgag cttttttgtt
     3541 ttcagaattg ttcatggtcc ccaacgtggc tgatagtcgc agtaatcgtt actcgttaac
     3601 cgacgcaggt cggtaccgtc aatgcggatg acataaattt cacatccgtg gtcatcgcca
     3661 tacttatcaa aataggcggt gaataccacc catttaccat ctggagaaaa tgagggaccc
     3721 tggctgttcc ctccggttgg agtgagctgg cgagcgtttg agccgtccgc attcatgata
     3781 tagagttcac gattccagga tggaccggaa tacgtgacaa tccattgccc gtcagctgac
     3841 caatcactgc ggccgcgaat ggccggcagg tttgagacct ggcgtaaacc atttccatct
     3901 cggttcacaa caaaaagctg caccttgccc tgggaactcg atgcatatag gatttgactc
     3961 ccgtctggtg accaggtcgg gtcccagccc aaaacctgcg agagacttct tgcctctcct
     4021 ccattgctgt tcatggtcat gacctgaggc tgacttccgg gatccccggt cgcgcgcgca
     4081 aatgtgattg aacttccatc cggagaaacc tctggcgcag ttaatatacc cagcctgtcc
     4141 gtcaattgat caacattccc gttactgaga gcaatgcgat agatttcata aacattttgt
     4201 tcacgaaacg aggcatagag gacgctttgc ccatcgggcg acaacgacgg ataaaagtga
     4261 cggacgttgt tatttgttgt taacctgcgg atgtttgaac cgtcagcatt cataacacaa
     4321 atttggtcga tggctgtcac tttgaaaatt tggcaggtga atacaatctg ccctgtgagt
     4381 tgatcggctg ctgatggagc tgggatggat gatccttcgg aaggaagcgt aggaaggatt
     4441 gtaggaaaac taaagtcagg cgttgtgaca gtatcaccgg gtgcgggcga ggatagcgat
     4501 gaaggagtcg atgcctgaag tgtgccgagt aagtcgggtg ttggggtcgc tgaaggccta
     4561 aaaagcccgc agcctacgat aaaaagaatc gttatgatta accacaaaaa ttttctcttc
     4621 tgcatgatcc tgatatcttt tcgcgcatag gtcgttggga gtttgtaatg ctacttcctc
     4681 aggccggtct ataagccgca ttctgtccga cacattgctg tgcctgtgcg atcatctctc
     4741 tgggcggcgc gtcgccgcgc cgctcggtgc ggcctacccg ggactggctc tctcaccgcg
     4801 tgaaagagca cgtatgaaga cgagcagtct cccgtcgtcc gcggacgact tcgtccctgc
     4861 ttggccttgc tcccggcggg ggttacctgg ccacccacat tactgtggat gccggtggtc
     4921 ttttacacca ccttttcact cttactaccc tcaccccatc ccctctccca gtgggagagg
     4981 ggcgcagggg tgagggagcg acctgtttct gtggcccttt tatccggcaa gttagctcct
     5041 cacggagggt tcctcgcccc gggtgctatc cgacgccgtg ctctatggag tgcggacttt
     5101 cctcgatccc gacaacgcag gaccgcgatc gcccaaccga cctgaggaca tattatctta
     5161 cacgtaagcc cacagtgcgt caacgttggt ttcgacattc aaatgaaatt tatacacttg
     5221 gctttatcga tcttgaattc tttttctcac atcgaccgac cgttatgaaa acaacaaact
     5281 ttgattggag aatttcacaa aagacattac aaagtgctgc agatattact taactgcaag
     5341 tttgttttag gtgacaaacc acttaaagcg tttacttatt gacgtgacca gcggtaaaat
     5401 agacttatac cctcgtattg gagactctgt gagcatattg cctgggaaat atgtgatcgg
     5461 tttgaccggt aatattgcga ccggtaaaag tgtcgtccgg cgcatgttgg aacaccttgg
     5521 tgcatatacc attgatgcag atgggttagc tcaccgcgtc atcgcaaagg gggcaccagg
     5581 gtatcagcct gttctggatc ggtttggtac atggcttctt gaaagcgacg ggcagatcaa
     5641 ccggaataaa ctggggcgag tggtatttgc cgatccggaa gcgctggctc aattggaatc
     5701 catcgttcat ccttatgtga gccaggccgt ggacttgctt gcccggcgtg cgacacaacc
     5761 cgtcgttgtg attgaa
//
LOCUS       DASTJP010000306         5188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_917203, whole genome shotgun sequence.
ACCESSION   DASTJP010000306 DASTJP010000000
VERSION     DASTJP010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917203"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="VFQ23_09180"
     CDS             complement(<1..1684)
                     /locus_tag="VFQ23_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9906803.1"
                     /translation="MKNSHTKEKHHDHSAMEHDQHIVAENKAPSEHSNHIEKKSSSHD
                     MHAAPMDHAAHGMGKEDEHAGHVDHSGHEDTFRRRFWISLLLSIPVLLYTPMLQMWFG
                     FRLPDFPGSQWVMPLFSVIVFVYGGMPFIQMAAPEIRSRQPGMMTLISLAISVSFIYS
                     LATMFVNLGEGFFWELVTLIDIMLLGHWIEMRSVRQASGALSELAKLLPDTAEHIMPD
                     GSIHTMPVSSLKVDNLVLVRPGASVPADGVVEEGESDVNESLITGESQPVKKNPGSKV
                     IGGTININGSLRVRVTATGDQTALAGIMRLVKEAQQSKSQTQILADKAAGWLFYLAIG
                     VAALTAILWTLSIGFDLFTLQRVVAVLVIACPHALGLAVPLVVAISTTSAAQNGILVR
                     NRLALETAREINVIIFDKTGTLTMGKQGVVAIKAANDGDETHALARAAALEADSEHII
                     ARAIHTAAVDKKLELPKVSGFEALNGRGVRGTVDGKEAWIGGPRMLESLDVKLDPVMA
                     KFTADAGAKGQTVVYLLDENNVSAAIALADVIRPESYEAIKRLHQMNVEVAML"
     gene            complement(1725..1943)
                     /locus_tag="VFQ23_09185"
     CDS             complement(1725..1943)
                     /locus_tag="VFQ23_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HET9906804.1"
                     /translation="MTTVTYSVPAMHCGHCIHTVEMEVGELQGVQTVKADLPTKKVQI
                     TFDTPATEDKIKALLAEIDYPVEGLISL"
     gene            complement(1969..2394)
                     /locus_tag="VFQ23_09190"
     CDS             complement(1969..2394)
                     /locus_tag="VFQ23_09190"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET9906805.1"
                     /translation="MKLKVLFSTLLIFLLSACTGTSQLETEITVEATDFTYSPLAIQI
                     PAGQPVTIMLKNSGNVEHDFVIDKINVKDVEAGDSGHAAHHQMGDQPNFDLHFFAEAG
                     ETATLKFTALEPGTYEIFCSVEGHKEAGMIGELIVASQE"
     gene            complement(2416..2685)
                     /locus_tag="VFQ23_09195"
     CDS             complement(2416..2685)
                     /locus_tag="VFQ23_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET9906806.1"
                     /translation="METENTIRRLKTIEGHLRGVIRMVEEDTYCIDVIRQIQAIDAAL
                     NKVSTQILENHLNSCVITAIKGNNPKERERVLKEITEVFEMSAKV"
     gene            complement(2875..3618)
                     /locus_tag="VFQ23_09200"
     CDS             complement(2875..3618)
                     /locus_tag="VFQ23_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906807.1"
                     /translation="MRRYVMSILLLTLTIAACSPMQQSTSEAPAEEIPATVPPEATDS
                     PLPAETKKPADPVEAMVVDRLARNLGLDKSEISVVSHENVEFSDACLGITMQDISCAQ
                     VTTPGHIIVLEADNLQYEYHTDRSGKRIQPATLALTWKRSGGIAGFCDNLMVFLSGEV
                     YAYKCDDPADAKIETFANLISARELEQFNAWMTNFGEVTLDASDPKGVSDRMEVILEL
                     YGVGRDKPGDEEQQEIIAWAQDLYQKLYS"
     gene            3688..4305
                     /locus_tag="VFQ23_09205"
     CDS             3688..4305
                     /locus_tag="VFQ23_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906808.1"
                     /translation="MRYRLLCLIVFIVTACSFPRANPNSAAETQQAIAATLAAIPAST
                     APPIPTLFPTPTQFDLAGLFCEYQFCIGHPVDMAFYDVSAQQNLGTPSSYAQGLIAAF
                     NATLFIQTVWQSAPGATDPQFMLDLILDPGLDTRLGTLEVKLIRDMNVLYTPITSTAT
                     PLLPYGGAAAWICGDRAFAWKVYTPDDQSAPTVFENSFSRFVCRR"
     gene            complement(4332..5081)
                     /gene="mazG"
                     /locus_tag="VFQ23_09210"
                     /pseudo
     CDS             complement(4332..5081)
                     /gene="mazG"
                     /locus_tag="VFQ23_09210"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
BASE COUNT         1282 a         1385 c         1103 g         1418 t
ORIGIN      
        1 tcaacatggc aacttccacg ttcatctgat gcagtctctt aatggcttca taactttccg
       61 ggcgaattac atctgcaagc gcaatcgcag ccgatacatt attttcatca agaagataaa
      121 cgactgtttg ccctttggca ccggcgtcag ccgtgaattt agccattaca gggtcaagtt
      181 tgacgtccag tgattcgagc attcgcggtc cgccaatcca tgcctcttta ccatcgactg
      241 tccctcgaac accgcgccca ttcagcgctt caaatcctga aactttgggt aactccaatt
      301 ttttatccac agcagcggtg tgaatggcac gagcaatgat gtgctcagag tcagcctcga
      361 gcgcggcggc acgtgcgagt gcatgggttt catctccatc attggccgct ttgattgcca
      421 ctactccctg cttgcccatc gtaagggtgc cagttttatc gaaaattatt acattaatct
      481 cacgtgctgt ctcaagcgcc aaacgatttc gcaccaggat gccgttttgt gcggcggatg
      541 tagtgcttat cgccactacc aggggcactg ccagacctaa tgcatgcggg caggcgatga
      601 ccaaaacggc gaccacgcgc tgtaacgtaa atagatcaaa gccaattgat agtgtccaca
      661 agatcgcagt caacgccgcg acgccgattg ccagataaaa taaccaacct gctgccttat
      721 ctgcaaggat ttgcgtctgt gatttacttt gttgcgcttc cttgacgagc cgcatgatcc
      781 cagccaatgc cgtttgatcg ccagtcgcgg tgactcgcac acgcagactt ccattgatgt
      841 taattgttcc accgatgact tttgagccgg gattcttttt tacgggttga gactctcctg
      901 tgatcaaaga ctcattgaca tcggactcgc cctcttccac cactccatct gccggcacac
      961 ttgctcccgg acgaaccaga acgaggttat ctaccttcag agacgaaact ggcattgtgt
     1021 ggatgctgcc atccggcatg atgtgttcag ccgtatcagg cagtagtttt gccagttcgc
     1081 ttagagcacc cgaagcttgt cgcacgctgc gcatctcgat ccaatgccct aacaacatga
     1141 tatcaatcag tgttaccagt tcccagaaga agccttcgcc caaatttaca aacatggtcg
     1201 ccaggctata aataaatgaa accgaaatcg cgagcgagat gagagtcatc atgccgggct
     1261 ggcgactgcg aatctcaggc gctgccatct ggataaacgg cataccacca tacacaaaca
     1321 caataaccga aaacaatggc attacccact ggctgcccgg gaaatctggc agcctgaatc
     1381 cgaaccacat ctggagcatg ggtgtataca gcaagacagg aatactcaac aacagcgaaa
     1441 tccagaaacg cctgcggaat gtgtcttcat gaccagagtg gtccacatgc ccggcatgct
     1501 catcttcttt gcccattcca tgtgccgcat ggtccatggg ggcagcatgc atgtcatggg
     1561 aactggactt cttctcgatg tggtttgaat gctctgaagg agctttgttc tcagccacga
     1621 tatgttggtc atgttccatc gcggaatggt cgtggtgttt ttccttcgta tgtgagtttt
     1681 tcatcttgcc tcgaattctt gagtaagtgt gttcagaaga gactttacaa cgaaatcaat
     1741 ccttccacgg gatagtcaat ttctgccagc aaagccttta ttttatcttc cgtggcaggt
     1801 gtgtcaaaag tgatttgaac tttcttggtt ggcaaatctg ctttgacagt ttgaacgccc
     1861 tgcaattctc caacttccat ttcgacggtg tggatacagt gtccacaatg catggcgggt
     1921 acagaataag ttacggtagt catcgtttct ccttaattag ataatttttt attcctgaga
     1981 ggcgacaatt aactcgccga tcatcccagc ttctttatgt ccttcaacgg aacaaaagat
     2041 ttcatacgtt ccaggctcca aagcagtaaa tttcagagtt gccgtttcac ctgcttcggc
     2101 aaaaaaatgc aaatcaaaat tgggctgatc acccatctga tgatgcgcag catgtccact
     2161 gtcgcctgct tcaacgtcct tgacgttgat tttatcgatc acaaaatcat gttcaacgtt
     2221 tcccgagttc ttcaacatta tcgtgactgg ttggccggca ggaatttgaa tggccaatgg
     2281 gctatacgta aaatcggtgg cctccacagt aatctctgtc tcaagctggg acgttcctgt
     2341 acaggcagat aacagaaaaa tcagtagggt gctgaacaac actttcaatt tcatcactaa
     2401 gtctccttca aaaacctaaa cttttgccga catttcgaat acttcagtaa tttccttcaa
     2461 tactctttcg cgttctttgg gattattccc cttaatagcc gtaataacac aggaattcag
     2521 gtgattctcc agaatttgtg tgctgacttt gttcaatgcg gcgtcaatcg cctgaatctg
     2581 gcggatcaca tcaatacaat atgtgtcttc ttccaccata cgaattacac cgcgcaggtg
     2641 tccttctatt gtttttagtc ttcgaattgt gttttcagtt tccataaggg cctcataatc
     2701 ctggacagaa cgagaattca atttcttacc cccccccagg ggggtggggt gcttggaata
     2761 ataaacgaaa acaatttgca tgtcaatact cacggtatca atgctttttg aaaataattc
     2821 tcaacatttc ccccgcaaat aaaagagcct gcaggtttca caggctctat ctcgctacga
     2881 atacaatttt tgatacaggt cttgtgccca ggcaataatt tcctgttgtt cctcatctcc
     2941 aggtttatca cgacccacac cgtataactc gagaatcact tccatgcggt ctgagacacc
     3001 tttcggatct gacgcatcca aagtaacttc accaaaattc gtcatccagg cgttgaactg
     3061 ttcgagctct ctcgctgaaa taagattggc aaaggtctct atcttcgcat ccgctggatc
     3121 atcacattta taggcataca cctcacccga tagaaagacc atcaggttgt cgcaaaagcc
     3181 tgcaattcca cccgaacgtt tccaggtaag tgcaagggtg gctggctgga tgcgcttccc
     3241 acttcgatct gtgtgatatt cgtattggag attgtccgct tccaaaacaa tgatgtgtcc
     3301 cggcgtcgtt acttgtgcgc agcttatgtc ctgcatagta atgcctaaac aggcatcaga
     3361 aaattcgaca ttttcatgac tgactactga aatttcactt ttatccagtc cgagattccg
     3421 agccaaacgg tcaacaacca tcgcttctac tggatccgcc ggtttcttcg tttccgcagg
     3481 caaaggcgag tccgtggctt caggcggaac tgtggcaggg atttcctccg ctggcgcttc
     3541 gctcgttgac tgttgcatgg gtgaacaagc tgcgatggtt aaggtcaaca gcaatatact
     3601 cattacatat cttctcattc gtactccttc acttgggatg acgcataggc cgagaatatt
     3661 gttccgaaaa actgtataat cgaggccatg cgttatcgtc ttttatgctt gatcgtattt
     3721 attgtaacag catgctcttt ccccagagcc aaccctaatt ccgcggcgga aacgcaacag
     3781 gctatcgcag caacgctggc ggccataccg gcctcgaccg ctcccccaat ccccacgctt
     3841 ttcccaaccc ctacacaatt cgatctcgca ggattgttct gtgaatatca gttttgcatc
     3901 ggtcatccgg tggacatggc attctacgat gtaagcgccc aacagaacct gggaacaccc
     3961 agcagctacg cccagggact gatcgcggcg ttcaacgcta ccctattcat tcagacggtc
     4021 tggcaatcgg cccctggcgc cacggaccca caattcatgc ttgacctgat tttagacccc
     4081 ggattggata cgcgcctggg cactttggaa gttaagctca ttcgcgatat gaacgtgctc
     4141 tataccccca tcaccagcac cgctacgccc ctgctcccct acggcggtgc cgcggcctgg
     4201 atctgtggcg atcgtgcctt cgcctggaaa gtttacacac ccgatgatca gtccgcacct
     4261 acagtgttcg agaactcatt tagtcgcttt gtatgcagaa gatagaacac aaaaatttgg
     4321 ttgggctcag gttataaccc cattttcttg ctcgtattcc aaagcgcatc catttctgcc
     4381 agagtcaaat ctgataagct acgcccttct ttttttactt ccttctccag ataaccaaag
     4441 cgctgtttga acttattatt agtgccgcgt aaggccgact ccgcatccac atgcttccaa
     4501 cgtgcgacgt tgaccagggc aaaaaacaaa tccccaattt cggaagcaag ctcaaaatcg
     4561 gactcggcgt tcttgatttc ttcaatctct tccttcacct tatcaaggac accttcaatt
     4621 tcaggccagt caaacccgac gcgcgcagcc cggtcttgat attcctgcgc ccgagacagt
     4681 gcaggcaaaa caagaggcac accatccagc aagcccttgt cttccttctt ttctcctctt
     4741 tccttttctt ttagtttttc ccaattcgcg agcacacctt ccacaccgtc taatttcaaa
     4801 tcaccgaata cgtgagggtg gcgtcgtaca atttttgaat ggattccctg tatcacctca
     4861 gtgatattga actgtccttc ttcgttagcg atttgtgatt gcaagacaac ctgcaataac
     4921 aaatctccaa attcttcgcg catggatttg aagtcgcccg aatcaattgc cgcgatggtt
     4981 tcataggact cttccagtag gtgcttacgc aacgtctcat gagtttgttc acgatcccac
     5041 gggcatccat tcggcgcgcg tagatgggcg atgatctcgg caaaggattc aaaagatgtt
     5101 cattcgccta aaggaggaac ataaatacaa gaattttcgg aaaattcaaa atcatttagt
     5161 cgttcttctt ttgtcattcc gttttgaa
//
LOCUS       DASTJP010000307         5741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1481869, whole genome shotgun sequence.
ACCESSION   DASTJP010000307 DASTJP010000000
VERSION     DASTJP010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481869"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFQ23_09215"
     CDS             complement(<1..212)
                     /locus_tag="VFQ23_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906809.1"
                     /translation="MQKLGRIAFITTPEYRDKHPEEIEDFVYRHLYFLCNWFEVLCTG
                     RTYSFVLGIANRSLDQVDPIRISEAT"
     gene            complement(292..1053)
                     /locus_tag="VFQ23_09220"
     CDS             complement(292..1053)
                     /locus_tag="VFQ23_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9906810.1"
                     /translation="MANNKNLPVLREKIAILIDGDNAQSSLLPQMLVEAGRHGQVTLR
                     RIYGDWTTNHMNSWKETLNYHAFQPIQQFRYTIGKNATDSAMIIDAMDILHSGVVDGF
                     CLVSSDSDYTRLATRIRETGIFVMGIGEKKTPKPFVNACDLFVYTENLVAETKAAQQK
                     RAPTSKTGKKKEEKEDSDPMPILSQAFEMAVGQDGWAPLAGMGQALYQLDPGFDPRTY
                     GHKQLSRMIANIKERFDMRTENLDGSNILYVRMKE"
     gene            complement(1068..1718)
                     /locus_tag="VFQ23_09225"
     CDS             complement(1068..1718)
                     /locus_tag="VFQ23_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906811.1"
                     /translation="MKRLVPTILVSILLTSCNGWVIQPFPGNPPTPFLPLTPTPSIFT
                     PTPVVIGVTSASATPNIPTATTIPTNTLFPISTDTSTITVTNLPAGQAILVDVLGCNT
                     SIDITHGMGEVTNAFVTMKNSGGVALTNLRVTLFALDEGREHPDKTIEITALPVGYEV
                     TLKLTVDSTYQQETPVQIEVTGDNGLFERVGSASCRDIGVLAPNPNGLYTPVPTTP"
     gene            complement(2096..2323)
                     /locus_tag="VFQ23_09230"
     CDS             complement(2096..2323)
                     /locus_tag="VFQ23_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HET9906812.1"
                     /translation="MYTTEHCSDCSRVKAFFRANDIPYLPVGLEGNTEATEFVVKINK
                     GHQSVPTIIFPDGSILVEPNWDELRAKLSQA"
     gene            2558..3430
                     /locus_tag="VFQ23_09235"
     CDS             2558..3430
                     /locus_tag="VFQ23_09235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9906813.1"
                     /translation="MTNLKRAFFWAAIYLAVIFVLGQTDYTGRPIINFASYFYLAVMI
                     AIPVTLFFPSISRVATFVPLAVWGGVYLIMLQLINRNLSANAEEFPVIILEFVLLEAG
                     VWFAHQLALQIGHAESIMDALALSAFPNRAHDIEAESQRIKIELTRSRRYHRPLSLIV
                     IDSESEDQKTTREMLKSVQQDLLNRFTSARVGQIIDDRIRQTDLVLRDHRGRFVILCP
                     ETALENTILLAKRISQAVKERTNLNVLWGVAAFPEEALTFDDLLQKASERLVHSEVPS
                     KEQVAVPVSEGSKL"
     gene            3442..4110
                     /locus_tag="VFQ23_09240"
     CDS             3442..4110
                     /locus_tag="VFQ23_09240"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9906814.1"
                     /translation="MISSYEEDWIKKFDPQKRLLTGTAYFVTKRILDLFLVLVSLPFW
                     LPLNGIIALIIRITSPGAPVIFTQLRTGKGGRRFHMYKFRTMVPEAEELKSKYSHLNE
                     LQWPDFKITNDPRITPIGRFLRKTSLDELPQLFNILRGEMSLVGPRPTSFGPETYKLW
                     HTSRLDVMPGLTGLWQIIGRAQLEFDDRLRLDIAYIERACIWLDINILFRTVMAVFES
                     RGAH"
     gene            4168..5304
                     /locus_tag="VFQ23_09245"
     CDS             4168..5304
                     /locus_tag="VFQ23_09245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9906815.1"
                     /translation="MEIKLYFRMLQKNWWLILLTAMTALTVSLAISYAAVPQFSSVAR
                     FIIIPSSTLTSGADVVRSLDTLDRRSVVATYAEVMNSTRILEGAAEFLGMNANEIILN
                     YQVQAVDLPDSSVLELTVSGPNPALAAELANAIGFQSISFTRGLNLIYELNFLDTAIP
                     AELPFSPEPLRDGLVALVLGTALGGGLAVISEQIRVPIEAYRQRRRIDSVTGIYNASY
                     FSRLLEEEVAQNPNDVLSIGIVELNGLSDYLGTLPPAGLQSLLVRVKEILRRELRGHD
                     IVGRWDEISFSVMLPTTDGPAASRTFDRIYQALSQPMDLSAYGVTVSLDPHIGGAVYS
                     NTITARELIEKAETSLEQSRRFSVKPIYVWEMKSPFWVQTDAQK"
     gene            5316..>5741
                     /locus_tag="VFQ23_09250"
     CDS             5316..>5741
                     /locus_tag="VFQ23_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906816.1"
                     /translation="MKQDLSVFFQSLNLNLPATWNPLLLAGFIVLALLAGGVAAVAGA
                     QLGWVALLAIPVLLFGLAILIRPELGLAAFVFIIYVQLNLVLAKVRPGIPSPAMLLLG
                     ALVFLIVWRMVVYGDRPLGWSRVSIILVVVIGWLFSVLAA"
BASE COUNT         1361 a         1299 c         1415 g         1666 t
ORIGIN      
        1 tgagtggctt cggaaattct tattggatca acttgatcta gagaacggtt ggcgatgcct
       61 aatacaaaac tataagttcg acctgtacaa agcacctcaa accaattaca tagaaaataa
      121 agatggcgat aaacaaaatc ctcaatctcc tcaggatgct tgtcccggta ttcaggagta
      181 gttatgaaag caatccgtcc aagcttttgc attattacct ccaagttcta tcgttagaat
      241 ttgtaatttg tgctgtattt acccgctggg aaatgatatt gcttctaaag cttactcctt
      301 catcctcaca tataaaatat tcgaaccgtc caggttttca gtccgcatat cgaaacgctc
      361 tttgatatta gcgatcatac gcgacagctg tttatgtcca taagtgcgtg ggtcaaagcc
      421 ggggtcaagt tgatagagtg cttgtcccat cccggctaaa ggtgcccacc catcctgccc
      481 cacagccatt tcaaacgcct gactgagtat cggcatcggg tcagaatctt ccttttcctc
      541 tttcttcttt cctgttttgc tggtcggcgc gcgcttttgt tgcgcggcct tcgtttcggc
      601 taccaggttt tctgtgtaaa caaaaagatc gcaggcgtta acgaaaggct tgggcgtctt
      661 cttctcgcca atgcccatca caaagattcc agtctcacgg atgcgcgtgg caagccgtgt
      721 gtaatcactg tcagaggaga cgaggcaaaa tccgtccacc acgcctgaat gaaggatatc
      781 catcgcgtcg atgatcatcg cgctgtcggt ggcgttttta ccgatggtat atctgaattg
      841 ttgaatcggc tggaaggcat gataattcaa cgtctccttc catgagttca tgtggttcgt
      901 ggtccagtcg ccatagattc ggcgaagggt cacttgtccg tgtcggccag cttcaaccag
      961 catttgcgga agcagggagg attgggcatt gtcgccatcg atgagtattg ctattttttc
     1021 tctgagcaca ggtaggtttt tattgttagc catgagggta ttatatatta tggggtggtg
     1081 ggaacagggg tgtaaagtcc gttggggttt ggggccaaaa cgccgatatc ccggcaggag
     1141 gcggaaccga cacgttcaaa caatccatta tcccctgtga cttcgatttg aaccggtgtt
     1201 tcctgttgat aggtactgtc gacagttagt ttcaatgtca cctcatagcc cacaggcaga
     1261 gctgttattt cgatcgtttt atccgggtgt tctcttcctt cgtcgagagc gaagagggtt
     1321 actctcagat tcgtcagggc tacaccacct gagtttttca tggtcacaaa cgcgtttgtg
     1381 acctcaccca tgccgtgcgt aatgtcaata ctggtattgc agcctaacac gtccactaaa
     1441 atggcctgtc cggcaggtag gttagtcact gtaattgtgg atgtatcggt gctgataggg
     1501 aacagggtat tcgtcggaat cgtggtcgca gttggaatat tcggtgtagc agatgcactc
     1561 gttacaccga tcacaaccgg tgtaggcgtg aaaatggatg gggtgggtgt taggggtagg
     1621 aacggcgtcg gtgggttgcc agggaagggt tggatcaccc agccattgca cgaagttagc
     1681 aaaattgaca ccaaaatcgt gggaacgagt ctcttcattt tctctcctcc cgatgattgt
     1741 atctctctta gggcaaatga gcaaagcacc cttttgtctg attttgagag cacacaatcc
     1801 aaccggatct tcgaccgggg gttcgttttt aggagaatta tctagcgata tggttgacca
     1861 tagatatttg agactcagct gcaaattcca gaatcatgaa tttgtattta ttggtggtat
     1921 tataggggca gccggtcatt tcctccgcaa tttgccatcc atcaagccaa aggagtggtt
     1981 aatcaatgcg agtatttccg ggttggggcc acccatacgg caactctcga ttgaacgttg
     2041 attgaaaatt taactggcga agcttcgaac ggcatggact cttatgccgc ggatttcatg
     2101 cctgtgagag ttttgctctc agctcgtccc aatttggctc caccaatata gagccatccg
     2161 gaaagataat ggttggcacg ctctgatggc ccttattgat ctttactacg aattctgtgg
     2221 cttccgtgtt tccttccaat cctaccggca gataggggat gtcattcgca cgaaaaaatg
     2281 ctttcactcg gctgcagtcc gagcagtgct cggtggtata cataacaatt tgagttggag
     2341 taattgtata gaggtccttc acgacgatcg attcctaaga gttttgaaag gattatggcg
     2401 ccaattgaaa agttagtgta taaaacgtaa cattctcttt taccaggcgt cttgtttctg
     2461 atactttaga catattaacg gcgattttct taccaaatcc cgttttccaa tactaatctt
     2521 tccattattg tcatcatcat atcaaagcga gagcgaaatg acaaacctaa aacgtgcatt
     2581 cttttgggcg gctatctact tggcagttat ctttgttttg ggccaaactg attataccgg
     2641 ccggcccatt atcaattttg catcgtattt ttaccttgct gtcatgatcg caattcccgt
     2701 tactttgttt ttcccgtcta tttcacgggt agcaacgttt gttccattgg cggtctgggg
     2761 tggtgtctat ctcattatgt tgcaattgat caaccgaaat ctttccgcaa atgcggaaga
     2821 gttcccagtg atcatcctgg aatttgttct attggaggct ggggtttggt tcgcgcatca
     2881 actggcacta caaatcgggc atgccgaatc aattatggat gcgcttgcgt tgagtgcatt
     2941 tccaaatcga gctcatgata tcgaggctga aagccagcgg attaagatcg agcttacaag
     3001 aagtcggcgt taccatcgtc ccctgagcct gatcgtgatt gattcagagt ctgaagatca
     3061 aaaaacgaca cgtgagatgc tgaaaagtgt ccagcaggat ctgttgaatc gttttacatc
     3121 ggcacgggtt gggcaaatca ttgatgatcg aattcgtcag actgatctgg tgctgcgcga
     3181 tcaccgcggc cggttcgtga tcctttgccc cgaaaccgct ctcgaaaata cgatcttgct
     3241 tgcaaagcga atcagtcagg ccgtcaagga gaggaccaac cttaacgttc tctggggtgt
     3301 ggcggcgttc cccgaggaag cccttacttt tgacgacttg cttcaaaagg cctccgaacg
     3361 cctggtccac tcggaagttc cgtcaaaaga gcaagtagcg gttccggtca gtgagggttc
     3421 taaattatga gtgtgttgga aatgatttct tcttatgaag aagactggat caagaaattt
     3481 gatccccaaa agcgccttct tacgggaact gcctatttcg taaccaaacg gatcctggac
     3541 ttgttccttg ttttggtttc attacctttt tggttgcctc ttaacggaat tattgcttta
     3601 atcattcgga tcacttctcc cggagcgccg gtgattttca cccagcttcg taccggaaag
     3661 ggtggtcgtc gcttccatat gtataagttt cgaacgatgg tgccagaagc ggaagagctc
     3721 aaatccaaat attctcatct gaatgagttg cagtggccag attttaagat cacaaatgat
     3781 ccacgtataa cacccatcgg gcgctttttg cgaaaaacca gtttggatga gttgcctcaa
     3841 ttattcaata tcctgcgggg cgagatgagt ttggttggtc cacgaccaac atcatttgga
     3901 cctgaaactt ataagctttg gcacacctcc agattggacg taatgccggg gctaacgggc
     3961 ttgtggcaaa ttatcggtcg tgctcagctt gagtttgatg accggctacg cctggatatc
     4021 gcttatatcg agcgcgcctg tatttggctg gacattaata tcctttttcg aaccgtcatg
     4081 gcagtcttcg aaagtcgcgg cgcccactga taaatccaag tataatgatc gaaatcccga
     4141 aataattgcc aattgagata atgtttgatg gaaattaaac tttacttccg catgctgcaa
     4201 aaaaactggt ggctgatctt gcttacggca atgacagctc ttacagtttc cctggcaatt
     4261 tcctatgccg ctgtgcccca atttagctct gtagcacgat tcatcattat tccttcatca
     4321 acattaacca gcggggcaga tgtggttcga agcctggata cgttggatcg tcgttcagtc
     4381 gtagcaacct acgcagaggt catgaacagt actcgtatcc ttgagggcgc tgcagaattc
     4441 cttgggatga atgccaatga aattatcctg aactatcagg ttcaagccgt agatttacca
     4501 gactcaagtg ttttggaact tactgtttcc ggtccaaacc ctgcattggc tgcggagctg
     4561 gcaaacgcga tcgggttcca atctatttct tttacgcgtg gtctaaacct tatttatgaa
     4621 ttgaattttc tcgataccgc tatcccggct gaattgcctt ttagcccaga gccacttcgc
     4681 gatggacttg tggccttggt gctgggtaca gctctcggag gaggacttgc ggtaatcagt
     4741 gagcagattc gcgttccgat cgaagcgtat cgacaaaggc gtcgtattga tagtgtcaca
     4801 gggatctaca atgccagcta cttctccaga ttattggaag aagaggtggc tcaaaatccg
     4861 aatgatgttc tatccatagg aattgtggaa ttaaatggac tatctgatta tctagggacc
     4921 cttccacccg ctggactgca aagtttatta gtgagggtca aagagattct gcgccgcgag
     4981 ttaagagggc acgatattgt cggtcgctgg gatgaaatca gcttttccgt gatgctgcca
     5041 acaactgacg gtccggcggc cagccgcacc tttgaccgta tctaccaagc cttgtcgcag
     5101 cccatggatc tttcggcgta tggcgtgacg gtgagtctcg atccccatat tggtggcgct
     5161 gtatatagta atacgattac tgcccgagag ttaattgaaa aagccgaaac ctctctggaa
     5221 cagtcgcgcc gatttagtgt caagcccatc tatgtgtggg aaatgaagag cccattctgg
     5281 gtgcaaactg acgcacaaaa gtaagatcga cccgaatgaa gcaggatctt tccgtttttt
     5341 ttcaatcact taaccttaat ctgccagcga catggaatcc attgttgctg gcaggtttca
     5401 ttgttttagc acttctcgca ggtggagttg cagcagtggc tggtgcacaa ttgggttggg
     5461 tggcgctgtt ggctatacca gtgctgttgt ttggcttggc gatcttaatc cgcccggagc
     5521 ttggcttagc agcttttgta ttcatcatat acgttcaact aaatttggtt ttggcgaaag
     5581 tacgtcccgg tattccgtca ccggcaatgt tattgttggg tgcactggtt tttcttatcg
     5641 tctggcgaat ggtcgtttac ggagatcgtc cgctgggatg gagccgtgtc agcattattt
     5701 tggtggtggt gattggttgg cttttttctg tccttgcggc g
//
LOCUS       DASTJP010000308         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_662710, whole genome shotgun sequence.
ACCESSION   DASTJP010000308 DASTJP010000000
VERSION     DASTJP010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662710"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFQ23_09255"
     CDS             <1..490
                     /locus_tag="VFQ23_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906817.1"
                     /translation="TVTIFVTDDRPNAEPRYRVRFYFDPNSITMASGNAHFIFNGLAG
                     TSTAILRVEFRFFSGAYQVRGRLVNDATTWTNTNWFTISNGPHSIELDWRASTGAGAN
                     NGGLTFWIDGTQQANLTGIDNDTRRMDRVRLGAVAGIDAGTSGTYYFDAFESRRQTFI
                     GP"
     gene            888..1172
                     /locus_tag="VFQ23_09260"
     CDS             888..1172
                     /locus_tag="VFQ23_09260"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HET9906818.1"
                     /translation="MSFQAYLDKVEEITGKTPNDFIAMAKEKNLTEHKDIIAWLKQEY
                     GLGLGHARAIAYIIQHGPEFTVRQTTGPHRDASGTLNLEGKRATKKKPTK"
     gene            1770..>2090
                     /locus_tag="VFQ23_09265"
     CDS             1770..>2090
                     /locus_tag="VFQ23_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906819.1"
                     /translation="MFAGTLPRGSWMIDRYENKMILYRPVGLQELALIYDSGMQAFPA
                     HLHQQPIFYPVLQLAYARQTASNWNVQHGEFAGYVTQFKVEDEYISDFEEHMVGGTQY
                     QELWI"
BASE COUNT          564 a          489 c          499 g          538 t
ORIGIN      
        1 caccgttacg attttcgtca ctgatgaccg tcccaatgcc gaaccacgct accgggtacg
       61 tttctatttc gacccgaact caattacaat ggcgagcgga aatgcacatt ttattttcaa
      121 cggtctggcg ggtacatcca cagccatcct tcgagtggaa ttccgcttct tctcgggggc
      181 ataccaggta cgaggcaggc ttgtcaacga tgcaacaaca tggacgaata ccaattggtt
      241 cactatcagc aatggacctc actctatcga attggactgg cgggcgtcta cgggggcagg
      301 tgcgaataat ggtgggctga ctttttggat tgatggcaca cagcaagcca acttgactgg
      361 catagataat gatacacggc gcatggatcg cgtgcggctg ggagcggttg ctgggatcga
      421 tgctgggacg agcggtacat actattttga tgccttcgag tcacgacgcc agacctttat
      481 tggcccttaa tatacatgca agagattcgt attgttttgc agatgcagta actacatttg
      541 tttgaaccag taagaaaatt gaacttgatg ggagtgtaaa aattcaaaaa tgccacgccc
      601 atcaactgct ctgtataagc ttgcgcttat gaaagggggc tagtaaaaga tttgctggcc
      661 ccaaacgatc tttgtagata attttctcgc ttcaagtttt ccgcctccaa gccgagcctg
      721 gccccgattg aatcccggta atgatatcat cgcaatatga ccatcacaac cgctcaggta
      781 atccaaatct ggcattcatc aagtcatcac aatagaaact ctattgaaat aaccgtacga
      841 cttgttaaca aagtactctt tagagagtac gcaaaaagga gaaaccaatg tcatttcagg
      901 catatttgga taaggtggaa gaaatcacag gaaagacgcc caatgacttc atcgccatgg
      961 caaaggaaaa gaacctaaca gaacacaaag acatcatcgc ctggcttaag caggagtatg
     1021 ggttaggctt ggggcatgcc cgggcgattg cttacataat tcaacatggt cctgaattca
     1081 ccgtacggca aactacaggt ccacaccgtg acgcgtcggg caccctaaat ctggaaggaa
     1141 agagagcaac gaaaaagaaa ccgactaaat aggggcatcc attcgcagtt aacgtttctc
     1201 tccagatgta gtgagtatga ttggaagtct cttcgacttc caatcattga attcagataa
     1261 tctttactga aatggatccg attacttttt ggcgttgatt tttgtcgaac tttcaatacc
     1321 aattcttcag tgctcccagc ctgtttgact tgcggtgcca caatggggga ttctaaagac
     1381 attctttttg ccaggccgat catgggattc ttgggaaccc ctggagcata tcgctcaaaa
     1441 atcttcaccg ctacgagttc cagcgcctcc gacagaccgc cctttacctt tgccttgcct
     1501 gaatgaagcc ctgggcagga tccagtttgc catctgcgac cgcgaaaata tcagctgtat
     1561 ctgccaaccc acagaggacc gcaggtctca ctcactatga ggtgtgcgta cgagacctgc
     1621 ggccactctt aagatggata gccgccgttt cattctgcag tctataggca gcctagcaga
     1681 cagttggatt ccatgttttc gaaagtaata gagaggctat agagatggta ttatttatgt
     1741 gccatttgaa tcaggtatat gctggatagg tgtttgcagg aactttgccg cgcggcagtt
     1801 ggatgatcga caggtatgaa aacaagatga ttctttatcg tccggttggt ttgcaggaat
     1861 tggcgctgat ctatgacagt ggcatgcagg ctttccctgc gcacctgcat caacaaccga
     1921 ttttttatcc ggtgcttcag cttgcctatg cccgccaaac cgcctccaac tggaatgttc
     1981 aacatggaga gtttgcaggc tacgtgaccc agtttaaagt agaggatgaa tacatcagtg
     2041 attttgaaga gcatatggtt ggcggaactc aataccagga actctggatc
//
LOCUS       DASTJP010000309         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1386936, whole genome shotgun sequence.
ACCESSION   DASTJP010000309 DASTJP010000000
VERSION     DASTJP010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386936"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..626)
                     /locus_tag="VFQ23_09270"
     CDS             complement(24..626)
                     /locus_tag="VFQ23_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9906820.1"
                     /translation="MKVFSIQGVKYVDHMNPPDPSWLISECSAGNDEAITTLVRQYET
                     EVFRLALSICNDVPEANEITQETFIAALRALPGYQEKKSFKSWLYTITINTARSHLRK
                     HKFAERLRQTLTSIFLVESQKSPSPEDVTIQNEQEAQVWKALNEMDEKHRIVTVLRYF
                     HELSITEIAEVLSIHEGTVNSRLHTTRERLRNTLEHLDKK"
     gene            complement(706..1791)
                     /gene="rlmN"
                     /locus_tag="VFQ23_09275"
     CDS             complement(706..1791)
                     /gene="rlmN"
                     /locus_tag="VFQ23_09275"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET9906821.1"
                     /translation="MLIYDLELVSLTNLLKEWNEPAYRAKQIWQGLYQHLYNAPEQFT
                     TLPKSLREKLAENTTFSPFSVKTYQDSSDGFTRKTLFELPDKNLIEAVLMRYGDPADN
                     PQITGSPTGRGAKNRRTLCISTQAGCAMGCVFCATGQMGFKRHLSSGEIIAQVMYYAR
                     ILKEENQVVTNIVLMGMGEPFHNYDNTMAAIDRLNDPDGYNFGARRFTISTVGLVPQI
                     RRFADEKRQVNLAISLHAADDESRVAMLPVNKRYKIDDVIEACRYYVEKTHRRITFEW
                     ALINGVNDTPEVAQKLAKRLKGLLCHVNAIPLNPTVGYSGQATSRERAAAFKESLEQA
                     GIPCTIRMRRGIDIQAGCGQLAGAVSS"
     gene            complement(1798..2583)
                     /locus_tag="VFQ23_09280"
     CDS             complement(1798..2583)
                     /locus_tag="VFQ23_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HET9906822.1"
                     /translation="MIPSHIKALILDMDGVLWRSDEPIGDLPEIFARIKSRGLKYVFA
                     TNNGTMTPEQYAEKLSRLGVDAEPRQVVTSALGAAHMLEQKFPKGTKVFMIGEEGIYQ
                     ALIGKGFEVLPVVDAQDAQVVVIGIDRAVTFDKMREATLLVRSGIPFYATNPDKTFPT
                     PRGEIPGAGAWISVVTTATEIEPIYAGKPYPYMMELSLEILDATREEALVIGDRLETD
                     IAAGQAVGCSTALVLSGVSTMEQAEQWVTPIDLIAKDLASLIE"
BASE COUNT          651 a          678 c          617 g          765 t
ORIGIN      
        1 gcatgtgtaa tttgcatgtc agtttacttt ttgtctaagt gctccagggt gttccgtagt
       61 cgttcccgag ttgtatgaag ccgggagttg accgtccctt catggatgga aagtacttcc
      121 gcaatctctg tgatggatag ctcatgaaaa tagcgcagca ccgtaactat ccgatgtttt
      181 tcatccatct cattcaatgc tttccatact tgtgcttcct gttcattttg aatcgttacg
      241 tcctcaggcg aaggcgattt ttgagactca acaagaaata tggatgtgag tgtctgcctc
      301 aatcgttcag cgaatttgtg tttgcgtaaa tgactgcgag cagtgttgat tgtgatggta
      361 tacagccagg atttgaagga ttttttctcc tggtaccctg gcaatgctcg tagagccgcg
      421 ataaatgttt cctgtgtgat ttcgtttgcc tctggtacgt cattgcaaat ggaaagtgcc
      481 agcctgaaca cctctgtctc atactggcgc acaagcgttg taatcgcttc gtcgttcccg
      541 gcactgcact ccgagatgag ccatgatgga tcagggggat tcatatggtc tacatactta
      601 acgccctgga tgctgaaaac cttcattctt gagttaaatg aattcgatac tgttaattgc
      661 gtgtagtcaa taatacctaa taaaaatttc tattaacttt aacaattaag aagagactgc
      721 gccagccaac tgaccacagc ctgcctggat atcgatccca cgtctcattc gtatcgtgca
      781 tggaattcca gcctgctcca gcgattcttt gaacgctgcc gcacgttcgc gggatgtggc
      841 ttgaccagaa tagcctacgg tggggttgag gggaatcgca ttgacatgac ataacaaacc
      901 tttgagtcgc tttgcaagtt tctgtgcgac ttcaggcgtg tcattgacgc cgttgatcaa
      961 tgcccattca aatgtaattc gtcgatgtgt tttttcaacg taatatctac aagcctcgat
     1021 cacatcatct attttgtatc tcttattaac gggcaacatc gcaacccgcg actcatcatc
     1081 tgccgcatgc agagagatcg cgagattcac ctgtcgcttt tcatctgcaa atcgcctgat
     1141 ttgtggaact agccctactg ttgaaatggt aaatcgtctt gcaccaaagt tgtatccatc
     1201 cgggtcgttc aaccgatcaa ttgccgccat cgtattatcg taattatgga acggttcacc
     1261 catgcccatt aatacgatat tggtcacaac ctgattctct tctttcaata tgcgcgcgta
     1321 atacatcacc tgcgcgatga tctcaccgct ggaaagatga cgcttgaagc ccatttgccc
     1381 cgtcgcgcag aagacacatc ccattgcaca gccagcctgg gttgaaatac aaagcgtcct
     1441 gcgattcttc gcccctcgcc cagtggggga gccagtgatt tgcggattat ctgccgggtc
     1501 accatatcgc atcaaaaccg cttcgatcaa attcttgtct ggcaactcaa acagagtctt
     1561 tcgtgtgaat ccatctgaag aatcctgata ggtttttacg ctgaaaggtg agaaggttgt
     1621 attctctgcc agtttctctc gcaaagattt tggcagagtg gtaaattgct caggcgcgtt
     1681 ataaagatgc tgatacaacc cctgccagat ctgcttggca cggtaagcag gttcgttcca
     1741 ttctttcaat aaattcgtga gcgatactaa ttctaaatcg taaatcaaca taggttctta
     1801 ctcaatcaaa cttgcaagat ctttggcaat gaggtcgatc ggcgttaccc actgttccgc
     1861 ttgctccatc gtagacacac cgctcagcac cagcgcggtc gagcatccca ctgcctgacc
     1921 tgccgcgata tccgtttcga gcctgtcgcc aatgacaagt gcctcttctc ttgtagcgtc
     1981 aagaatttcc aacgataatt ccatcatata aggataaggc ttgcctgcgt agatgggttc
     2041 aatctctgta gccgttgtga caactgatat ccaggcgccc gcgccaggga tttctccgcg
     2101 cggggtggga aaagtcttat ctggattcgt cgcataaaag ggaatgccac tccgcacaag
     2161 taatgtcgct tctcgcattt tgtcaaacgt gacggcgcgg tcaatgccta tcaccaccac
     2221 ttgagcatcc tgcgcgtcta ctacagggag gacttcaaat ccctttccaa tcagtgcctg
     2281 atatatgcct tcctcaccga tcataaagac ttttgtgccc tttggaaatt tctgctccag
     2341 catatgcgca gcgcccaagg cggaggtcac tacctgccga ggttcagcat caacgccaag
     2401 ccttgaaagt ttctccgcat attgttccgg agtcattgtg ccgttgttcg tagcgaagac
     2461 gtatttcaag ccgcgagact ttatgcgcgc gaagatctca ggtaaatcgc caatgggctc
     2521 atctgatctc cagagaacac cgtccatgtc gagaatgagg gctttgatgt gagagggaat
     2581 catatgttat ctcggtggtt gagtagacac gagcgtagcg agtgtcgtat cgaaaccaca
     2641 aatttattgg aaaattatat cgtaagtaat tctgagattg ccacgtcgcc cctctctgcg
     2701 gggctcctcg c
//
LOCUS       DASTJP010000310         9124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_772431, whole genome shotgun sequence.
ACCESSION   DASTJP010000310 DASTJP010000000
VERSION     DASTJP010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772431"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFQ23_09285"
     CDS             <1..454
                     /locus_tag="VFQ23_09285"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9906823.1"
                     /translation="TAELGHVWHLSQSHAYTILKRLESRGEISAHIVEQEKLPARQVL
                     RITAQGRRRFFEWLELGIGTNARSIRLEFLTRLYFTQLHRPENTSQIFHAQLAEIESR
                     VEKLESLLENLPPDQIFNRLSLDLRLQQMKLIKNWIVEIRTQFHMVRR"
     gene            457..1257
                     /gene="modA"
                     /locus_tag="VFQ23_09290"
     CDS             457..1257
                     /gene="modA"
                     /locus_tag="VFQ23_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9906824.1"
                     /translation="MKRFLSLILLSTLLVAGCAPAVNDTQTSTSEARALTVFAAASLT
                     DAFTEIGANFEAANPGVTTTFNFAGSQALRTQIEEGAPADVFASANQTEMEALVISEH
                     VVAGTPQVFLSNKLVVVVPADNPAALENLEGLARSGVKLVIAAEEVPVGKYTREALNL
                     MNDQFGSDFKDKLLANVVSNEDNVRQILAKVQLGEADAGIVYMSDAVSAPELQTIEIP
                     NDLNVIATYPIAALLDTANSDLATAFVEYVLSSDGQAILEKWGFGPPE"
     gene            1336..2133
                     /locus_tag="VFQ23_09295"
     CDS             1336..2133
                     /locus_tag="VFQ23_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906825.1"
                     /translation="MKRLVNDRHLIWIFIIPSIILFALFALPFFALFERSLTTDFFGY
                     AFSDQALQALRLSLVTSTITTVVTIILGTPFAYLLARWRFRFKPWLELLIDLPIVLPP
                     SVAGLSLLIAFGRRGLFGSALNVIGISLPFTTAAVVLAQTFVAAPLFVRSARVGFTEV
                     DKQLEEAAQVEGANHWQLFKEVMFPLAGRALLSGAILTWTRALGEFGATILFAGNLEG
                     ITQTMPMAIYLGFESEIGVALALSVMLVGVSALLLGIIRKLEREEER"
     gene            2337..3905
                     /locus_tag="VFQ23_09300"
     CDS             2337..3905
                     /locus_tag="VFQ23_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9906826.1"
                     /translation="MENKTLPFINPATGAKFGEVKPATPEAVESAVHEMRQSFAIWSK
                     KPLRERIRILRKFQTLLIDKRDEITNVLNQDCGKSRQDGLLELLVTVDMLAQYCAQAP
                     KWLKKKRVSSGLYLFKQCFVEYRPYGVVAVLAPWNYPFTLALPPVLAALLAGNTVVLK
                     PSEVTAATGVLIEQLIQGVPELAPFVRVVHGDGRVGAALTEAKPDYIFVTGSTPTGKK
                     ILQAAAENLIPVGLELGGKDAMIVLDDAEIDAAARWGVWGAFFNCGQTCMAVERVYVV
                     ESKYEEFLKRSVSYAKGLKTGFTNEFDSPYFMGPITDPRQAEIIDAHLEDALSKGARV
                     LTGGKAQGGFYNPIILVDVDHSMQIMQEETFGPVMPIMKVKDEAEAIELANDNVFGLG
                     ASVWSQNMTHAKNVADKVQASSVILNDSIAQFGIPMLPFGGTKDSGFGRIHGQEGLMQ
                     FTRPFSFAVGGAPVKWDIATVLRKNGHYDLATAIMGVIFGTTLRQKWEALSQLFRRKP
                     SIQSQKVSVQSKAD"
     gene            4021..4263
                     /locus_tag="VFQ23_09305"
     CDS             4021..4263
                     /locus_tag="VFQ23_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4054..4056,aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HET9906827.1"
                     /translation="MLKIEIVYCAVUHYLGRAVSLAEELLKNYEHVIETLTLLPSEGG
                     RFEVTVNGQLLFSKQQLHRHAEPGEVLGLVRKMVGE"
     gene            4386..5372
                     /gene="trpS"
                     /locus_tag="VFQ23_09310"
     CDS             4386..5372
                     /gene="trpS"
                     /locus_tag="VFQ23_09310"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET9906828.1"
                     /translation="MPKKGRVFSGARPTGRQHLGNYLGAIKNYVALQNEYDCVYCIVD
                     VHALTTVETTQDLRQNTNEMVLDWLAAGLRPDDSIIFVQSHVPEVMELHTYLSMVTQM
                     GKLTDLPTFKEKLAQQPENANYGLLGYPVLMTADIVLYKTDIVPVGIDQAPHLEFARE
                     IVRSFNYRYNTKVLIEPQVKHTEVLKVLGIDGKAKMSKSLNNHIELASTPEETTKRVR
                     EMVTDPARIKRTDPGNPDVCNVFSMHKLFSSTEEIDMVNVECRRAGIGCVDCKMLFAK
                     NLNKHLEPFRARRADLASQPDYVSDVLKDGGKRARVLAQQTMEEVREAMQLP"
     gene            5415..5855
                     /gene="dtd"
                     /locus_tag="VFQ23_09315"
     CDS             5415..5855
                     /gene="dtd"
                     /locus_tag="VFQ23_09315"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET9906829.1"
                     /translation="MMRVLIQRVSKASVTVEGQTISSIGKGLLILLGVGHGDAEEQTA
                     FLAEKTANLRIFEDEQGKTNLSILDVKGEAIVVSQFTLYADTRKGRRPSFIDAALPDI
                     AEPLVNRFVEVLRSHGVPTQTGKFGAHMEVEIHNDGPVTIWLEK"
     gene            5852..6340
                     /locus_tag="VFQ23_09320"
     CDS             5852..6340
                     /locus_tag="VFQ23_09320"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET9906830.1"
                     /translation="MKRQPNALQRVIHQIVMVRPVTALFAPIIHRVDQAVLKLTGGKY
                     TISEFAGWNIIQLTTIGAKTNQPRTIPLVGIFDNEKVALIASSFGREHNPAWYYNLKA
                     NPHCHVAFKGKSREYIAREAKGEEREQYFQMAISQYGGYQKYEERASHRQIPVMVLEP
                     KT"
     gene            complement(6315..7130)
                     /locus_tag="VFQ23_09325"
     CDS             complement(6315..7130)
                     /locus_tag="VFQ23_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906831.1"
                     /translation="MKRDLYFSKPLMNAAGSLGFYPDFRRLREAPPKQSEGGEDFGSL
                     FGAFVTNPFSLRPRLPAAKPDLIEYPGGFLLHTGLPNPGLSTGIKRYSAKWSRSDLPI
                     IVHLMADRPEETQRMVRSLETQENVMAVELGFAPLLADDIILLTLEMCLGELPLVFSL
                     PVEQVLRLGPRLIQDGAQAISIAAPRGALSPRLLGEGGMRHVITGRLYGPSLFPQTLE
                     TIYSAVKIGLPIIGSGGVYSKESADAMLSVGALAVQVDAALWNPASMFSAPAP"
     gene            complement(7186..8502)
                     /locus_tag="VFQ23_09330"
     CDS             complement(7186..8502)
                     /locus_tag="VFQ23_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9906832.1"
                     /translation="MADQYFVAVIGAGPAGLFGARELVNLGARVALFNRDIKPGGLAE
                     YGIYPNKYTMKNGLRKQFRQIMDLPNLDYYGNVTVGSHGDLTLDDLRALGFQAILVTA
                     GAQGTKWLGLPGEELEGVYHAKDVVYYYNNLPPYSQKSFRFGKRCAVIGAGNVMLDIA
                     HFLSREEKVEEVIAVVRRGPAEIKFDKKEMEYVIASLDQAALDAEIQRVAPIMQAVGQ
                     DPEAARASILEALPKALPKTSDTRFRFEFLASPVQMIGDAEGRLTQVELEDNILVASN
                     GDTKAKGTGNKRRLDVETVVFAIGDKVDDSFGLPVEWNEFVKNQRPKFPVDGTSYESP
                     FEDVFVGGWARKASSGLVGIARKDGTNAAKAVWQYLQQTKQPSEANGKAIAVKLKSLS
                     KPIVLKEDVKKLEAMEAAETQKRGIEEFRFNNNDEMLQAMGLEVTA"
     gene            8851..>9124
                     /locus_tag="VFQ23_09335"
     CDS             8851..>9124
                     /locus_tag="VFQ23_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906833.1"
                     /translation="MTQPVSNQNEPSKLKQLLNGGVDESIKSRLPRAEGMRAAPPEPM
                     AIPVEETEATPAAEPEPKRRQFKFLPAFWTIASVMSFTVNIVMLIVV"
BASE COUNT         2291 a         2282 c         2318 g         2233 t
ORIGIN      
        1 taccgctgaa ttggggcacg tctggcattt gagtcaaagc catgcatata cgatcttaaa
       61 acgcctcgag agccgcgggg agatttcggc gcacatcgtg gagcaggaga aactccctgc
      121 acgacaggta cttcgaatca ctgcgcaagg acgccggcga ttcttcgaat ggcttgaact
      181 gggaatcggc acaaatgccc gctcgatccg ccttgaattt ctcacccgct tgtatttcac
      241 tcaacttcat agaccggaga acacgtcaca aatcttccat gcacagcttg ctgaaatcga
      301 atctcgggtt gaaaagctcg aaagcctgct tgaaaatctg cctccggatc aaatctttaa
      361 ccgacttagt ctggaccttc gcctccagca aatgaagctc ataaaaaatt ggatcgtaga
      421 gataagaaca caatttcata tggttaggag atgaagatga aacgttttct atcgctgatc
      481 ctgcttagca cgttgttggt agcgggatgt gcaccagccg tcaatgatac tcaaacttct
      541 acgtccgaag cacgcgccct cactgtcttt gccgccgcat ccctcaccga tgcatttacc
      601 gagatcggtg caaacttcga ggccgccaat cccggtgtga ccaccacttt caactttgct
      661 ggctcgcaag ccttgcggac ccaaatcgaa gaaggcgcac cagctgatgt atttgcttca
      721 gcaaaccaga cggagatgga ggctctggtc ataagtgagc atgttgtggc aggtacacca
      781 caggttttcc tttcaaacaa gctggttgtc gttgtacctg cagacaaccc ggctgcactg
      841 gagaatctcg aagggctcgc caggtcaggg gtcaagcttg tgatcgctgc ggaggaagtt
      901 ccggttggaa agtatacgcg tgaggcgctg aacctcatga atgatcagtt tggaagcgac
      961 ttcaaggaca agctgcttgc caatgttgta tccaacgaag acaatgtcag gcagatcctt
     1021 gccaaggtcc aacttggtga ggcggatgcg ggcattgttt atatgtcaga cgcggtgtca
     1081 gcgcctgagc tgcaaaccat cgagatcccg aacgacttga atgtgatcgc tacatatcct
     1141 attgcggctc tgttggatac tgccaattca gatctcgcga ccgcttttgt ggaatatgtc
     1201 ctttcctctg atggacaggc tatcctggag aaatggggat ttgggccacc agagtaaacg
     1261 tccttgcggc tactgattgc gtcaacacat ccaccgggga cttttctttg ttcaatgaga
     1321 taatccagag tgcttatgaa acgattggtc aatgatcgtc acttgatttg gattttcatt
     1381 atcccaagca taatcctatt cgcattgttt gctcttccgt tcttcgcgct cttcgagcgt
     1441 tccctcacta cagatttttt tggctatgca ttctcagatc aggctctaca agccctgcgc
     1501 ctcagcctgg ttacaagcac aatcactaca gtcgttacga taatcctcgg tacgccattc
     1561 gcctacctgt tagcgcgctg gcgatttcga ttcaaaccat ggctcgagct gttgattgat
     1621 ctaccgattg tccttccacc ttctgttgca ggattgtcgt tgttgatcgc gttcggtcgc
     1681 agaggacttt ttggttcagc cttgaacgtc attgggatca gcctgccttt tacgactgcg
     1741 gccgtcgttc ttgcacaaac atttgtcgcg gctccactgt ttgtgagatc cgcacgggtt
     1801 ggcttcacag aggtggataa acagctggag gaagccgcgc aagtcgaagg agcaaatcac
     1861 tggcagttgt tcaaggaggt catgttcccg ctggcaggcc gcgcattgct cagtggtgca
     1921 atcctaacgt ggacacgcgc gctgggagaa ttcggcgcaa cgatattgtt cgccgggaat
     1981 ttagagggaa ttacgcagac catgccgatg gccatctatc tgggattcga aagcgaaatt
     2041 ggcgtcgccc ttgccttgtc cgtgatgctg gtgggggtct ctgcactctt acttggaatc
     2101 atacgaaaat tggagagaga agaagagcga tgataagctg atgggtaatg agagaaattg
     2161 attctgagaa tgagccctgc tctgcagcca gcaaacttta gagacgaagc aaatccttga
     2221 cttcatgagc ggtgaaaatc ccagtaaaat ccagggcggg ctataattgc gtatcatcaa
     2281 ttggcactat tcacacgatt aacagtacac gtctgcctgt tttcccggag ttatttatgg
     2341 aaaataagac cctgcctttc atcaacccgg ccactggagc caaattcggc gaagtcaagc
     2401 ctgcgacacc cgaagctgtg gagtccgcgg tgcacgagat gcgccagtcc tttgctatct
     2461 ggagcaaaaa gccattaaga gaacgaattc gtatcttgcg taaatttcag acgctgctca
     2521 ttgacaaacg cgatgagatt acaaacgttc tcaatcagga ttgcggaaag agtcgtcagg
     2581 acgggttact cgagctatta gtcactgtgg atatgctcgc tcaatactgc gctcaggctc
     2641 cgaaatggct gaagaaaaaa cgagtctcct ccgggctgta cctttttaaa caatgcttcg
     2701 ttgaatatcg tccgtatggc gtggtagctg tgctcgcgcc ctggaattac ccttttactc
     2761 tcgcgctccc tccagtattg gcggccctat tggctggaaa taccgtagtg cttaaaccat
     2821 cggaggtcac tgcggcaacg ggagtgttga ttgagcaatt gatccagggc gttccagaac
     2881 tcgctccctt cgtgcgtgtc gttcacggcg atggccgcgt tggtgccgcg ttgaccgaag
     2941 ccaaaccgga ctacatcttc gtcactggct ccacgcccac cggtaagaag atccttcagg
     3001 ctgctgctga aaatttaatc ccggtaggac tcgaattggg cggcaaagat gcgatgatcg
     3061 tattggatga tgcggagatt gatgccgcgg cgcgttgggg agtatggggt gccttcttca
     3121 actgtggaca aacctgcatg gccgtcgaac gtgtctatgt ggtggagtca aaatacgaag
     3181 aatttctaaa gcgttcggtg tcttatgcaa agggactcaa gacgggtttc actaatgaat
     3241 tcgatagtcc atattttatg ggtccaataa ccgacccgcg ccaggcagaa attattgatg
     3301 cacatctgga agatgcactt tcgaaaggag cgcgcgtgct tacaggcggt aaggcacagg
     3361 gcggatttta caatccgatc atacttgttg atgtagatca ctccatgcag attatgcagg
     3421 aggaaacctt tggaccggta atgcccatca tgaaggtcaa agatgaagcg gaagcaatcg
     3481 aactggccaa cgataacgtc tttgggcttg gggcttcggt ttggagccaa aatatgacac
     3541 acgcaaaaaa tgttgcagac aaggtacaag cctcttctgt catcctaaat gacagcatcg
     3601 cgcagtttgg aattcctatg ctcccatttg gcggtaccaa agattcaggc tttgggcgta
     3661 tccatggaca ggaaggcctg atgcaattca cgcgaccctt tagctttgca gtgggcggcg
     3721 cgccggtaaa gtgggatatc gctaccgtcc tgcgtaagaa tgggcactat gatctggcaa
     3781 ccgcgattat gggtgttatt ttcggtacaa cactccggca aaaatgggaa gccttatctc
     3841 aattgttccg tcggaaaccg agtatccaat cacaaaaagt cagtgtgcag tcaaaggctg
     3901 actaaaacac ttcaataacc aatcggtcat tatgtgttca tagcaacttt gaatagcgag
     3961 aatcttttta atagctattt ttgccgaatg atataatctc tcgaaatttg cggagggatc
     4021 atgctgaaaa tcgagattgt ctattgcgcg gtctgacact atctgggccg ggccgtgagc
     4081 ctggcggaag aattgctgaa aaactacgag catgtgatcg aaactctcac cctcctaccc
     4141 tcagaaggtg gcagatttga agtgaccgtc aatggtcaat tattgttttc caagcagcag
     4201 cttcatcgcc atgccgaacc tggcgaagtg ctgggattgg tcaggaagat ggttggtgag
     4261 taatattctg ttggtcgagt agtgcggagc acgtatcgag actaacaagt tacatgaaac
     4321 gaaaatattt cttgaaatct ggtggtctcg aaactacttg accaccgaaa tgctgaggat
     4381 attgaatgcc taaaaaagga cgtgtgtttt cgggggccag accaacagga aggcagcatc
     4441 ttgggaacta tctgggcgcg atcaagaatt acgtcgcgct gcagaatgaa tacgattgcg
     4501 tgtactgcat tgtggatgtc cacgcgctaa caacggttga gacgacccag gaccttcgtc
     4561 aaaacaccaa cgagatggtg ctggactggc tcgcagccgg acttcgaccg gacgattcca
     4621 tcatatttgt tcaatcgcac gtgccggaag ttatggagct tcacacctat ctttcaatgg
     4681 tcacgcaaat gggcaagttg acggacctgc caacattcaa ggaaaaactc gcgcagcagc
     4741 ctgagaatgc caattacggt ttgttaggat acccggtgct gatgacggct gacatcgttt
     4801 tgtataagac ggatattgtc ccggtgggaa tcgaccaggc ccctcacctt gagtttgcgc
     4861 gcgagatcgt gcgctccttt aattaccgtt acaacacgaa ggttttgatc gaaccccagg
     4921 tgaagcatac cgaagtgctg aaggtgctgg gcatcgatgg caaagccaag atgagcaaaa
     4981 gtttgaacaa tcacattgaa ctggcttcca cgcctgaaga gacgaccaaa cgcgtgcgcg
     5041 aaatggtgac tgacccggcg cgcatcaaac gcacggatcc cggtaacccg gatgtctgca
     5101 acgtgttctc catgcacaag ctcttctcct cgactgaaga gattgatatg gtcaatgtgg
     5161 aatgtcgccg cgcgggaatc ggctgcgtag actgcaaaat gctgtttgcc aaaaacttga
     5221 acaagcatct tgaaccattc cgtgcccggc gcgctgatct ggcatcccaa ccggattatg
     5281 tctctgatgt gttgaaggat ggcgggaaac gagcacgcgt ccttgcgcaa cagaccatgg
     5341 aagaagtgcg tgaggcgatg cagttgccgt aaatgaattg taggggcacg acatgtcgtg
     5401 cccttacgca tttaatgatg cgtgtattaa tccagcgagt ttccaaagcg agcgtaacgg
     5461 ttgaaggcca gaccatttcc agtattggaa aaggtctttt aatattgttg ggagtcggtc
     5521 atggtgatgc agaagaacaa acggcatttc tcgccgaaaa gactgcaaac ctgcgtatct
     5581 ttgaagatga acaggggaaa acaaatttat cgattcttga cgtgaaaggg gaagcgatcg
     5641 tagtctcgca gttcacgctt tatgcagata cgcgcaaagg gcgccgtcct tctttcatcg
     5701 atgcggcttt gcctgatata gcagaaccgc tggtcaaccg atttgttgaa gtgttacgca
     5761 gtcacggcgt tccaactcaa accggaaaat tcggcgcgca catggaagtt gagatccaca
     5821 atgatggacc ggtgacgatc tggctcgaga agtgaagcgc caaccgaatg cacttcaaag
     5881 ggtcattcat cagatcgtga tggtccggcc ggtaacggcc ctctttgcac ctattatcca
     5941 tcgagtggat caggccgtac taaaactgac cggagggaaa tatacaatct ctgaatttgc
     6001 gggctggaac attattcaat tgacaaccat tggagcaaag acgaatcagc cgcgcaccat
     6061 tcccctggtt ggaattttcg ataatgaaaa ggtcgcgctg attgcgtcga gctttgggcg
     6121 cgagcacaac cctgcctggt attacaacct caaagccaac ccacattgtc atgttgcgtt
     6181 caaaggcaaa tcaagagaat atattgctcg ggaggcaaaa ggcgaagagc gtgagcagta
     6241 ttttcagatg gcgatttcac aatacggtgg gtatcaaaaa tatgaagagc gcgcttcaca
     6301 ccgacagatc cccgtcatgg tgctggagcc gaaaacatag aggctggatt ccacaatgcg
     6361 gcatccacct ggacggccag cgcgccgacc gaaagcatcg cgtcggcgct ttcttttgag
     6421 tagacgccgc ctgagccaat aatcggtagc ccaattttta cagcggaata aattgtttcg
     6481 agtgtttgtg ggaatagcga gggaccatat aggcggccag ttattacatg cctcattccg
     6541 ccctctccca gtaggcgagg ggaaagagcg ccgcgcggcg cggcgatgct gatggcttgt
     6601 gctccatctt gtatcaaccg cggacctaac ctcagcactt gctccacggg caaagaaaaa
     6661 accagaggca actcacccag gcacatttct aatgtgagca aaataatgtc atctgcaagc
     6721 agtggagcga agccaagctc gacggccatc acattctctt gcgtttccaa tgagcgcacc
     6781 atgcgctgag tctcttcagg gcgatccgcc ataagatgga cgatgatcgg caaatctgaa
     6841 cgcgaccatt tggccgaata tctttttatt cctgtgctca gcccgggatt tggcaaacct
     6901 gtatgcaaaa ggaatccgcc tggatattcg atcaagtctg gtttcgcggc gggcaggcgc
     6961 gggcgtagcg agaacgggtt cgtcacgaac gcaccgaata gacttccgaa gtcttcgccc
     7021 ccttcgcttt gtttcggggg tgcttcgcga agccttcgga agtctggata gaatcctaat
     7081 gatcccgccg cgttcatgag tggtttgctg aaatacaagt cacgtttcat atttcaattg
     7141 tacttttaaa aaataaaccc ctcttgagca aagaggggca tctgcttacg ctgtcacttc
     7201 caaccccatt gcctgtaaca tctcatcatt attattgaac ctgaattcct ctatcccgcg
     7261 cttctgtgtt tcggccgctt ccatggcttc cagttttttg acatcttctt tgaggacaat
     7321 cggtttgctc agactcttta gtttcacagc aattgccttc ccattcgctt ccgaaggttg
     7381 ttttgtttgt tgaaggtatt gccagactgc cttcgccgcg ttcgtcccat ccttgcgggc
     7441 gatgcccacc agacccgagc ttgccttgcg tgcccagcca ccgacgaata cgtcttcaaa
     7501 aggcgactca taggatgtgc catcgaccgg aaactttggg cgttgattct ttacaaactc
     7561 attccactcc acgggcagac cgaaagagtc gtccactttg tcgccaatcg cgaagacaac
     7621 cgtttccaca tccaggcgac gcttgttccc tgtgcctttg gcttttgtat cgccgttgct
     7681 tgctacaagg atattgtcct ccagttcaac ctgggtcaac ctgccttcgg catctccgat
     7741 catttgaaca ggggaagcca ggaattcaaa tcgaaagcgc gtgtcggatg ttttaggcaa
     7801 cgctttcggg agagcttcca aaatcgaagc gcgcgccgcc tccgggtcct gaccgaccgc
     7861 ctgcatgatc ggagctacgc gttgaatctc agcatccaat gccgcctgat caaggctggc
     7921 aattacatat tccatttcct tcttatcaaa cttgatttca gccggaccgc gtcgaactac
     7981 tgcaataact tcctccacct tttcttcacg gctgagaaag tgagcgatat cgagcatgac
     8041 attgccggcg ccaattacag cacagcgttt tccaaagcgg aatgatttct gactgtacgg
     8101 aggcagattg ttgtaatagt acacgacgtc ttttgcatga tagacccctt caagctcttc
     8161 cccgggcagt cccagccact tggtaccctg cgctccggca gtcaccaaaa tcgcctggaa
     8221 gccgagcgcg cgcaggtcgt cgagtgtgag atctccgtgt gagccaactg tcacatttcc
     8281 gtaatagtca aggttgggca ggtccatgat ctgacggaac tgcttgcgca aaccattttt
     8341 catcgtgtac ttgttggggt aaattccata ttcagcaagc ccacctggct tgatatcgcg
     8401 gttgaacaac gcaacccgcg ctcccagatt gacaagttcg cgcgcaccaa atagacccgc
     8461 gggtccagcg ccgatcacgg ctacgaagta ttgatctgcc acaatgcctc cgaaaagttc
     8521 tacccgaaaa taataagtgc gggctgatta tagccaaaag gaaattcacc tacaagacga
     8581 tctctcattc ttttggtaac atcaagccag tattgccgaa aaagtgtctc tcgaaatact
     8641 atatctatgt taagattgcg gcgtagtggc cacatgtggc atcggctctt ctaccttttc
     8701 agtgaatttc cagtctataa tttacctgga aaagtttagc aaatgatgag atcagtgtag
     8761 cgcggtactg cggacaagcc atcactaact gcttgtcaat agcagacaaa ttcaaaatgc
     8821 aagcggtggt tctctgatcg aggaggtctt atgactcaac cagtatccaa tcaaaacgag
     8881 cccagcaaac tgaaacaact gctgaacggt ggagtggacg aatccatcaa atcacgcctg
     8941 ccgcgggcag agggaatgcg tgccgcacca ccggaaccga tggcaattcc agtcgaagaa
     9001 actgaagcca cgccggcggc tgagccagaa cccaaacgac gacaattcaa gttccttccg
     9061 gcattctgga cgatcgccag cgtgatgtcg tttaccgtga atatcgtaat gttgatcgtg
     9121 gtac
//
LOCUS       DASTJP010000311         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1197506, whole genome shotgun sequence.
ACCESSION   DASTJP010000311 DASTJP010000000
VERSION     DASTJP010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197506"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFQ23_09340"
     CDS             <1..220
                     /locus_tag="VFQ23_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906834.1"
                     /translation="TAYTAKGGEYIVNVGLTGHFEGTNHHGGKIVLDNARQLEAGEVL
                     DLTADIKPNYDFFKGGPGYAAKDVDQGM"
     gene            complement(277..2697)
                     /locus_tag="VFQ23_09345"
     CDS             complement(277..2697)
                     /locus_tag="VFQ23_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET9906835.1"
                     /translation="MNRVTQSNLLKIAAVFLVLQALIMTLSPGVRARTWETDFRWSQW
                     IALAIWGFFTWRTHQAILKRLPDADPYLFPAAALLSGWGLLTVWRLDTTFGARQSIWL
                     GVSMLVLYFGTRLPTSLDSIRKYKYLLLTAGMLLTGLTLFFGTNPLGFGPRLWLGCCG
                     VYFQPSEPLKLLLVAYLSAYLADRLPIRLGALPLLYPTLLLSGLAILLLYFQRDLGTA
                     SIFLALYTSIIYLATQRRRAVLVSMGFLILVGFAGYYFVDIVHARIASWLNPWHDPSG
                     GSYQIIQSLMAIANGGVEGRGPGLGSPALVPVAISDFIFAAIAEETGLIGTVGLITLF
                     GLILARGLRTAIRAPNLFRRLFAAGISAYIGIQALLIVGGNLRLLPLTGVTLPFISYG
                     GSSLLVSFIALLFLLLISNHLDEEPAPLLRPQPYIALGSFLSLGLFAAALANGWWAVV
                     RGPDLLARTDNPRRVIEDNFVMRGMLLDRTNIIINSSEGEVGSYERVYKYPDLSAVAG
                     YNHPVYGQAGLEASLDEYLRGLQGNPASVIWWNHLLYGMAPEGLDVRLSIDLYLQYRT
                     DQLLIDHIGAAILMNAASGEIFVMSSHPTFDPNQLNELGSELKDDPDKPLINRATQGL
                     YPIGSLIDPFSNAISGQQTPGENELENIYELLGFHRSPQLRIQVAEPLKNVDVMDLHV
                     SPLQVALAAAALSNHGIAPTPRIATAVNTPEEGWVVLQSLGTSFHAIPAESADATAES
                     LMVDGESYWSHLGSAQADDSSVTWFIAGTLPNWQATPLVVVVLLEEENAQLAEWIGRE
                     VLVDAMNP"
     gene            complement(2701..>3470)
                     /locus_tag="VFQ23_09350"
     CDS             complement(2701..>3470)
                     /locus_tag="VFQ23_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HET9906836.1"
                     /translation="FGKTSAPIVVGNLENERVAFLARHGIGHHITPSEVPYRANIYAL
                     KSLGVQRIISISACGSLKEELAPGHIVIPDQIYDHTHGRARSFFEDGMVAHISVADPF
                     CFDLSNQLESAVSGAGGVVHRNGTFITIEGPRFSTKAESNLYRSWGMSIIGMTASPEA
                     FLAREAEICYATMAHVTDYDVWHTSEAPVTVEMVIQTLSKNTTLAQEAVRILARNLHK
                     ERPCECGHALASALITRKEVVPPPTRQKLDLLVNKYL"
BASE COUNT          933 a          888 c          900 g          749 t
ORIGIN      
        1 tacggcctat acagccaaag gcggcgaata catagtgaat gtgggtctaa caggtcactt
       61 tgaaggcacc aatcaccacg gcggaaagat cgtcttggat aatgccaggc aactagaagc
      121 cggcgaagtg ctggatctca ctgccgatat caaacccaac tacgatttct tcaaaggtgg
      181 tcccgggtac gctgcaaaag acgtagacca gggaatgtaa ccctcctaaa gaaatatgtc
      241 atattttgcc ccgcacttta gtgcggggca atttgtttag ggattcatgg catccacgag
      301 cacttctcgc ccgatccact cggccaattg tgcgttctcc tcttccaaaa gcacaaccac
      361 caccaggggc gttgcctgcc aattgggtag cgttcccgca ataaaccatg tgaccgacga
      421 gtcgtctgct tgtgcactgc ccagatgcga ccaatagctt tcaccatcta ccatcaggga
      481 ctctgccgtt gcatccgcgg actcggctgg aatcgcatga aaagacgttc ctaaagattg
      541 caggaccacc cagccttctt ccggagtatt taccgccgtt gcgatacgcg gcgtgggagc
      601 aattccgtga ttgcttagcg cggcagctgc caaagcgact tgtagtggtg aaacatgcaa
      661 gtccatcaca tctacatttt ttaaaggttc tgcgacctga attcgtagtt ggggagagcg
      721 atggaagccc aaaagctcat agatattctc caactcgttt tcacccggag tttgttgtcc
      781 agaaattgcg tttgagaaag ggtcaatcag tgagccaatt gggtagagcc cctgggtggc
      841 gcggttgata agcggtttgt caggatcatc tttcaattct gagccgagtt cgttgagttg
      901 attggggtca aaggtcgggt gagatgacat gacgaagatt tcgccgctag ccgcgttcat
      961 caaaattgcg gctccaatat ggtcaatcag caattgatcg gtgcgatatt gaaggtaaag
     1021 gtcaatgctc agacgtacgt ccaacccctc aggagccatt ccataaagta agtgattcca
     1081 ccagattacg gaagctggat tgccttgcaa gcctcgaagg tactcatcca acgaggcttc
     1141 tagaccagct tgtccataga ccggatgatt gtatcctgcc acagctgaaa ggtctggata
     1201 cttatagacc ctctcataac tacctacttc accttcggat gagttaataa tgatattcgt
     1261 gcgatccaat agcatcccgc gcataacgaa gttatcctcg atgacgcgtc gagggttatc
     1321 ggtacgtgcc aaaaggtccg gaccccgaac cacggcccac cacccattgg ctagtgcagc
     1381 cgcgaataac cccaatgata ggaaggagcc aagcgcgata tacggctgag gccgcagaag
     1441 cggagccggc tcttcatcca aatgattgct gatcaaaaga agaaataaca gggcgataaa
     1501 agaaaccaac aaggaggagc ccccataaga gataaagggt aaggtgacac cggtcagtgg
     1561 aaggagacgc aggttgcctc ccacaatgag taaggcctga ataccaatat aggctgaaat
     1621 acccgcagcg aacagtcggc ggaagaggtt gggagctcga atcgcagttc gcaaccctcg
     1681 agcgaggatc aagccgaaca aggtgatcaa ccctactgtg ccgatcaggc ctgtttcttc
     1741 cgcaattgct gcaaaaatga aatcggaaat tgccacgggg acaagggccg ggcttcccaa
     1801 accgggaccc cgtccctcca ccccaccatt tgcgatggcc atgagcgatt gaataatttg
     1861 gtacgaacct ccgctggggt catgccatgg attgagccag gaagcgatcc gagcgtgcac
     1921 gatatccaca aaataatagc cagcaaaacc aaccaatatc aggaacccca tactgaccaa
     1981 taccgcccgc ctgcgctgcg ttgccagata gatgatgctt gtatacaggg ctaggaatat
     2041 tgaggcggtg ccaagatcac gctgaaagta caagagtaaa atagcgagcc cactcaaaag
     2101 caacgtaggg tagagcagcg gcagcgcgcc gaggcggatt ggaagacgat ctgcaagata
     2161 cgccgagaga taggcaacca gcaaaagttt aaggggttcg gagggttgga agtacacccc
     2221 gcaacaccca agccacaatc gcggaccaaa tcctaaagga tttgtcccaa aaaagagtgt
     2281 caaaccggtg agtagcatgc ctgcggttag aagcagatac ttatacttgc gaatggaatc
     2341 taaagaagtg gggaggcggg ttccaaagta aagaacgagc atgcttactc ccagccaaat
     2401 cgactggcgc gcgccgaaag ttgtatcaag tcgccagacg gtgagcaagc cccatccgct
     2461 taaaagtgcg gctgcgggga ataagtatgg gtcggcatcc ggaagtcgtt ttaggattgc
     2521 ctggtgtgta cgccatgtga agaatcccca aattgcgaga gcgatccatt gtgaccagcg
     2581 aaaatctgtt tcccatgttc gggcgcgtac tcctggtgaa agagtcatga tcagcgcctg
     2641 caaaacgaga aaaacagccg cgatcttcag caggttactt tgcgtcacac ggttcatttg
     2701 ctataagtat ttattgacca ggagatccaa tttctgacga gttggtggag gaactacctc
     2761 tttcctcgtg atcagagcag acgccagggc atgtccacat tcacaaggcc tttccttgtg
     2821 caagttgcgg gccaggatcc gtaccgcctc ctgcgccagc gttgtatttt tgcttagcgt
     2881 ttgaatgacc atttccactg tgaccggtgc ctcgctcgtg tgccagacgt cataatctgt
     2941 gacatgagcc attgtggcgt agcagatttc agcctcccga gcaagaaatg cctcgggtga
     3001 ggccgtcatt ccgataattg acatacccca cgaacggtat agattggatt ctgcctttgt
     3061 cgaaaagcgg ggaccttcga ttgtgatgaa cgtcccgtta cgatgaacaa cgcctccagc
     3121 cccgctgacg gcggattcca attggttgga aagatcaaaa caaaatggat cggctacact
     3181 gatgtgggca accatcccat cctcaaaaaa cgagcgcgcg cgtccatggg tatgatcata
     3241 aatttgatca ggaatcacga tatgacccgg agctagctct tccttcagag atccgcaagc
     3301 gctgatgctg atgatacgtt gcacacccag tgacttcagc gcataaatgt tggctcgata
     3361 aggaacctcg ctcggcgtga tgtgatgccc aattccatgg cgggcaagaa aggcgacccg
     3421 ttcattttcc aaattaccca ccacaattgg tgcgctggtc ttcccaaagg
//
LOCUS       DASTJP010000312         6313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1300517, whole genome shotgun sequence.
ACCESSION   DASTJP010000312 DASTJP010000000
VERSION     DASTJP010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300517"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFQ23_09355"
     CDS             complement(<1..716)
                     /locus_tag="VFQ23_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906837.1"
                     /translation="MPSNLPLRYGVNPQQAPARVFTATDSDLPFTILNGSPGYINLLD
                     ALNSWQLVRELKAVLNLPAAASFKHVSPAGVAIAAPLSDGLKNAYFVDDLELSPLAAA
                     YARARGADRLCSFGDWIALSDTADESVARLIAREVSDGVIAPAYEPAALEILKNKKQG
                     KYAVIQIDPSYEPSSNLEIRQVFGITFEQRRHDRLVAPSDLTNIVTTKKDVSESALRD
                     LILAWVTLKYTQSNSVCFVT"
     gene            1292..6175
                     /locus_tag="VFQ23_09360"
     CDS             1292..6175
                     /locus_tag="VFQ23_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HET9906838.1"
                     /translation="MKKNQHTSLKTEDDQGSGAGTFNWLTAADTKTWLDERDACALVA
                     SVRKNGEATHGNLKRALAALSIMGHRSGEVNGEGDGCGVLTDIPRLLWAQALEQEGKP
                     GWLAEDRRFFVGHLMIPNTNTDLNIKAKVIGMLTESGVDVLLERPIQTRPQALGKMAR
                     MQAPHLWQIAGLVRNGPLENVERTLFDLALKIEHCTKVAVASLSSYVVVYKVRGAIET
                     LYQYYPELRNPDFRTAITIGHARYSTNTATSFERVQPFNLLGHNGEINTIARLREQSQ
                     MLGVELVDGGSDSQDLDRLLETLIHRYHFTLMEAMEIAFPPILSEVEKLSPDLQAIYK
                     YYRQAFGPFAQGPAGIISRFGDECVFSVDALGLRPLWFGETEKEFFFSSEKGVYHLDT
                     MRLDPIPLSPGEKMRIRVRRREDIEVSDYPAIQQRMLKLTLRRFGSLETINKRLARAT
                     FKTGSTQAATSNEEIHPSAKINLDNRMSAFGWGREDREWIQELAKNGADPISSLGFDG
                     PLASLSKERQNISDYFKEAVAVVTNPAIDREREVEHFSTQIVIGERPPLVPGELGQAT
                     TLTFDSPLLVDDPKYFSLKPPSSAAVSIAKDGDGSRNVKDLFEHIENETGYPSLNGVV
                     QQYSEEQVKEIQTVTLQDESTAQALERIAQTAVEAVRGGSQLIILNDADSFREQNGWL
                     DPILVVGIVDRALRQTFIETPPATSPTPIPLGDNGKIDLGLVASTQQINLRRQVGVIL
                     RSGAIRNLHDLIMCIGMGADAVVPYLILEASLNDATLTHEGQIERLKNTLKALRSGIE
                     KCTSTMGIHESRGYGRLFACIGLSNDIALSLGASNYGGSDEGGLKWEDLDTDLTSRKA
                     AYHSPGRGELSRVNHFYPKIWKAAGKLGKGEADWRSYEEHVENTAKSTPVTIRNVLDF
                     NFPEESTVQPDKVDLGITSHRLPFLIASMSFGSQGEVAYRAYAEAAFRLNMLSLNGEG
                     GEIPDLLGKYPFNRGIQIASGRFGITAETINATNLIEIKVGQGAKPGEGGHLPARKVT
                     QKVAAARHARPGVDLISPSNNHDIYSIEDLAQFIEELKTINPKARVAVKVPVVPGIGI
                     IAIGIAKADADIIYLTGYDGGTGAARSHSLRYVGLPAEIGVVDAHRALTDSGLRDKVE
                     IWADGGVRSASDVIKLMCLGANRVGFGTLPMVAIGCTICRDCQLGTCHTGITTQLETT
                     EQAHASGVKHFVPRDFEQAVEALVNVFNGLGEAMQATIARLGFEHAQDIVGRSDLLQQ
                     ISHHEKIDLREMLVTVDEYLNTKPIPVTLPVHEMADIDRGPLHRPRNHLTTVISNLVM
                     ESFTTYQQDAIRFEDDKVTPVDRALGTHLTGALTRFSHKWSWAPGHGGVGGHRESWRQ
                     PVDELDIQSVPTTALRFFSSSVPGNGLGAYNADPVRIIVEGGAQDGVAKGISGGRVII
                     LKGYNHDGKLIDGSVGKGLAYGGTAGIVIVQGNADSRACIRLSGADVVIGGEITKPLN
                     DSLGFIGARANIKGFLCEYMTSGRVVVLGDPGPWMCAGMTGGVLYLRLQPHLNFDQAA
                     IQRRLAKGAKVQVQDVGETDLKNLRELLTIYAEELRHNHQSEEAQKIVDLLVDWKNTF
                     VRVVPLGQQEEQRFATE"
BASE COUNT         1539 a         1615 c         1746 g         1413 t
ORIGIN      
        1 tccgtaacga aacaaacgga attggattga gtgtatttga gggtgaccca ggcaaggatc
       61 aagtcacgca gggcggattc ggacacatcc tttttggttg tgacaatgtt cgtgagatcg
      121 gacggcgcga ccagacggtc atggcggcgc tgttcaaagg tgatgccaaa cacctgacgg
      181 atttccaggt tggaggaagg ttcatacgag gggtcaattt gaatcactgc atatttgccc
      241 tgctttttat ttttgagaat ttccagcgcg gcgggttcat aggcaggcgc aatgacgccg
      301 tctgaaactt cgcgggcgat caggcgcgca accgattcgt ccgccgtatc agatagtgca
      361 atccaatccc cgaaggaaca caatcggtct gctccacgcg cgcgggcata ggctgcggca
      421 agcggagata attcaagatc atccacaaaa tatgcgtttt tgagaccgtc tgaaagcggc
      481 gcggcaatgg caacccccgc agggctgaca tgcttgaacg aggccgcggc ggggagattc
      541 agcacagctt tgagttcgcg gacaagctgc caggagttca aggcgtccaa taaattgata
      601 tacccaggcg agccgttgag gatcgtgaat ggcaaatccg agtcagtggc cgtaaaaacg
      661 cgcgccggcg cctgttgcgg attaacgccg taacgaaggg gaagattgga tggcatattt
      721 acccgtttcc ttgtcttgtt gtttcgttac ccaaagcgtt caatgacttt aaaaaatcga
      781 aatcggtccg ccatacgttt cctttcgggg cgcaagatcg tatcttgcgt tacgcgacac
      841 gaaaaaaaac accctccggc cttggagagc gtttggtgat acaggcagag agaactgtgg
      901 tgaagatttt cctgtttcct gtttaccaaa acatgcccca acctgacaac cacagtcgcc
      961 gcagaaaaca gaactcatgg agattgggcg gtattctact acgcactaaa aatacgtcaa
     1021 tgttaatgga gtacccattt ggataaaaat gcttatccaa gaagtaccca tataggtaat
     1081 agcaatttcg ttttcaggct gtattattgg aagaatttat caattcataa cttaccaatt
     1141 tcaatacccc tccgttggtc aggtaacccc ttgtcgttcg cgggatatat ggtgaccaac
     1201 agttatatga aacaaaagat agattgaata tttgtggtct cgctacgctg cgtcgccact
     1261 tgaccatcaa tgactgaaaa agaggtgagc catgaaaaag aatcaacata catcattaaa
     1321 aacggaggat gaccagggat caggagctgg tacattcaac tggttaaccg ccgcggatac
     1381 aaagacgtgg ctcgatgaac gcgacgcttg tgctctggtc gcatccgttc gtaaaaatgg
     1441 ggaagcgaca catgggaatc taaaacgcgc attggccgcg ctgagcatta tgggacatcg
     1501 cagtggtgag gtaaacggcg aaggcgatgg ctgcggagtg ctaaccgata tcccgcgttt
     1561 attatgggca caggcactgg aacaagaggg aaagccgggc tggctcgccg aagaccgtcg
     1621 ctttttcgtc ggtcacctaa tgatccccaa cacaaatacc gacctcaata tcaaagctaa
     1681 agttattggc atgttgactg aaagcggcgt ggatgtatta ctcgaacgcc ccatccaaac
     1741 ccgcccgcag gcgctgggca agatggcgcg catgcaggct cctcacctct ggcagattgc
     1801 cggattagtc aggaatggtc ctcttgaaaa tgtggagcga acattattcg acctggcact
     1861 gaaaatcgag cactgtacga aggtggcggt tgcatcgttg tcgagttatg ttgtggtcta
     1921 taaagtgcgc ggggcaattg agacgctgta tcagtattat cccgagcttc gaaaccctga
     1981 tttcaggacg gccatcacca tcgggcatgc gcgctattcc actaacactg ctaccagctt
     2041 cgaacgagtc cagccattta atctgcttgg tcataacggt gagatcaata cgattgcgcg
     2101 cctgcgcgaa caatcacaga tgctgggtgt tgagttggtt gacggtggca gcgactcgca
     2161 ggatttggat cgcctgcttg agacgctcat ccatcgctat cacttcacgc tgatggaagc
     2221 aatggaaatt gccttccctc ccatcttgag tgaagttgaa aaactctctc ccgacctgca
     2281 agctatctac aaatattacc gtcaggcgtt tggccccttc gcgcagggac cggccggcat
     2341 catctcgcgc ttcggcgatg aatgtgtttt tagcgtggat gcattgggct tgcgcccatt
     2401 atggttcggt gagacggaaa aagaattctt cttctcctca gagaaaggtg tctatcatct
     2461 ggatacgatg cggctggatc ccatccccct ctcacccgga gagaagatgc gcatccgcgt
     2521 ccgccgccgc gaggatatcg aagtgtcaga ttatccggcg attcaacagc gcatgctcaa
     2581 gttgacgttg cgccgatttg gttcgttgga gacgatcaat aagcgactgg cgcgcgcgac
     2641 gttcaagacg ggttcaacac aggcggctac tagtaacgaa gagattcacc catcggccaa
     2701 aatcaacctc gataacagga tgtctgcatt tggctgggga cgcgaagacc gcgagtggat
     2761 ccaggagttg gcaaagaacg gcgctgatcc aatcagttcg ctcggctttg atggtccgct
     2821 ggcgtcgctt tccaaagaac gacaaaacat ttcagactat ttcaaggagg cagttgccgt
     2881 tgtcaccaat cccgccattg accgtgaacg cgaagtggaa catttctcca cacagatcgt
     2941 gatcggtgaa cggccgccct tagttcccgg tgaactcgga caggcaacga cgctgacgtt
     3001 tgattcgcca ctcctggtgg atgatcccaa gtatttttcg ctcaagccgc catcctccgc
     3061 ggctgtttcc atcgcaaagg acggcgatgg gagcaggaac gtaaaagatc tttttgaaca
     3121 catcgaaaac gaaactggct atccatctct caacggggta gttcaacaat actccgaaga
     3181 gcaagtcaaa gaaattcaaa ccgtaacctt gcaggacgag tccacagccc aagccctgga
     3241 gcggatcgct caaacggcag tcgaggcggt tcgaggcggc agtcaattga tcatcctgaa
     3301 tgatgcggat tcatttcgcg aacaaaacgg ctggctggat ccgattctcg tggttggcat
     3361 tgttgaccgc gcgcttcgtc agactttcat cgaaacgccg cccgccacct cgccgacacc
     3421 aatcccgttg ggcgacaacg gcaaaattga tctggggctc gtcgcatcga ctcagcaaat
     3481 caatttgcga cgacaggtcg gcgtcatttt gcgcagtgga gcgattcgca atttgcatga
     3541 tttgatcatg tgcatcggca tgggcgcgga cgcggtcgtg ccttatctta ttttggaagc
     3601 cagcctcaac gacgcgactc tgacgcacga aggacagatc gagcgattga agaacacgct
     3661 caaagctctt cgctcgggca ttgaaaaatg taccagcacg atgggcattc atgagtcacg
     3721 cggatacggg cgtttgtttg cgtgtatcgg cttgagcaac gacattgctt tgtcacttgg
     3781 ggccagcaac tacggaggtt cggacgaagg cgggttgaaa tgggaagatc ttgataccga
     3841 tctcacatca cgcaaagccg cctatcacag cccggggcgg ggcgagcttt cacgggtcaa
     3901 tcacttttac ccgaagatct ggaaagccgc aggcaagctt gggaaaggtg aagctgattg
     3961 gcgttcgtat gaagagcacg tggagaatac cgccaaatct acaccggtga ccattcgtaa
     4021 tgtcctcgat ttcaattttc cggaagagtc aactgttcag ccagacaaag tcgatttggg
     4081 aatcacttcc caccgattgc cattcctgat cgcatccatg tccttcggct cacagggtga
     4141 agttgcctat cgggcttatg ccgaagccgc gttccgcttg aatatgcttt ccctcaacgg
     4201 cgagggcggt gagattcccg accttcttgg aaagtacccg ttcaaccgcg gcattcaaat
     4261 tgcatcgggt cggttcggca tcaccgctga aaccatcaat gccacaaacc tgatcgaaat
     4321 caaagttggg cagggagcca aaccgggcga aggcggccat ttgccggcgc gcaaggtcac
     4381 tcaaaaagtg gcagccgcgc gccacgcgcg tcccggcgtg gatttgatct ccccctctaa
     4441 taatcacgac atctattcga tcgaagacct ggcacaattc atcgaagaac ttaagaccat
     4501 taaccccaaa gcgcgggttg cggtgaaagt tccagtggtg cccggcatcg gcatcatcgc
     4561 catcggcatt gccaaagcag atgcggatat catttatctc accggctatg acggtggcac
     4621 cggcgcagcg cgttcgcatt cattgagata tgtggggtta cctgccgaaa tcggcgtcgt
     4681 ggatgcacat cgcgcgctca ctgattccgg cttgcgcgac aaggtagaga tctgggcgga
     4741 tggcggcgta cgctccgcgt cggatgtgat caagctgatg tgtctcggcg cgaaccgcgt
     4801 cggcttcggg acgttgccca tggtcgccat cggctgtacg atttgtcgcg actgtcagtt
     4861 gggcacttgt catacgggaa tcaccacgca gcttgaaacc acagagcaag cccatgcaag
     4921 cggcgtcaag catttcgtgc cgcgtgattt cgagcaggca gtagaggcac tggtcaacgt
     4981 gttcaacggt ttgggggaag ccatgcaggc aaccattgca agacttggct ttgagcatgc
     5041 acaggatatc gtggggagat ccgacttatt acagcaaatt tctcatcatg aaaaaatcga
     5101 cctgcgcgag atgctggtca cggtggatga atatctgaac accaaaccga tccccgtcac
     5161 attacctgta catgaaatgg cagatattga tcgcgggcca ctgcatcgtc cgcgcaatca
     5221 tttgacgacg gtcatttcaa atctggtgat ggaaagtttt acaacctacc agcaggatgc
     5281 catccgtttt gaagatgaca aagtgacgcc tgtcgaccgc gcgctgggga cgcatttgac
     5341 tggtgcgctc acgcgtttca gtcacaagtg gagctgggca cccgggcatg gaggtgtggg
     5401 tggtcatcgc gaatcgtggc gtcagcccgt ggatgaactg gatatccaat ctgttccaac
     5461 gacggcactg cgattcttca gcagttctgt tccgggcaat ggactcggtg cgtataacgc
     5521 tgaccccgtg cggatcatcg tcgagggcgg cgcgcaggat ggggtcgcga aaggcatctc
     5581 cggcggacga gtcatcatcc tcaaaggcta taaccatgat ggtaagttga tcgatggctc
     5641 agttgggaaa ggtctggcat acggtggcac cgcgggtatt gtcatcgtcc agggcaacgc
     5701 agactcgcgt gcctgcattc gcctctcagg tgcggatgtg gtcattggcg gcgaaatcac
     5761 caaaccgcta aatgatagtc tcgggtttat cggggcgcgc gccaacatca agggctttct
     5821 ctgtgagtat atgacttccg ggcgcgtcgt cgtgttgggt gacccgggtc cgtggatgtg
     5881 cgcggggatg acgggcggtg tgctctattt gcgcttgcag ccgcatctca atttcgatca
     5941 ggctgccatc caacgcagac tcgccaaggg ggcaaaggtc caggtgcagg atgtaggtga
     6001 aactgatctc aagaatttgc gcgagcttct aacgatctac gcagaagagc tcaggcacaa
     6061 tcaccaatcc gaagaggcac agaagatcgt cgacctgtta gtggattgga agaatacatt
     6121 cgtgcgcgtt gtcccgttgg gtcagcagga agaacaacgg ttcgcgacag agtaaaggcg
     6181 ggatgacccc gcccctcctc agaataaaac tccctggcaa tgccggggag tttgctgctt
     6241 atatggttcc tcttaaccgc taagttcgcc gagaacgcaa aggaaaaact gttaaagctt
     6301 cgcggtcttc gcg
//
LOCUS       DASTJP010000313         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_639151, whole genome shotgun sequence.
ACCESSION   DASTJP010000313 DASTJP010000000
VERSION     DASTJP010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639151"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1946
                     /locus_tag="VFQ23_09365"
     CDS             <1..1946
                     /locus_tag="VFQ23_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906839.1"
                     /translation="TMDRLIGGPGVFQEMFRIDRPARGPLFEAYYQIFGIQPFPYHMS
                     SFLWRVAGGLAALWLFGQLWPRQRLATFIMAVLFALYPGYLRWMEGFENQPRILSSFL
                     EALSIALTLQAIRTPRTILKILTWIGSILTGWAYIALVDFAFGMEVFRLLCVFLLVNR
                     DQENLPFVKRSISAIRAWGIAALIPFGFLFWRFFLFHNERQATDVGLQLSYLFASPLT
                     TGAMWLLRLVQSTFNVTVPAWGSSLFQSLFEIPLPNSMLGIWIAGIVVVLFLGISFSI
                     EKMDNNPTQLDVVASQTWQVEAIWVGLAGVVAGVMPVIVANRYVSVEAYSHYALPASL
                     ASVMIVVGILSLINLRPVRLGAAALLVILAVLTHYSVSLRILHEESVIAQFWQQVVWR
                     SPGLKAGTTLFVSYPSVSYGEDVDAVAGPANFLYFPEQTNQIPAVYPLIALPQMDYTT
                     IYVLRGGQERASGYRTHVGEIDYDNMLVISQPTEDACVHVIDSQWPRYSNEDSAQILL
                     LGQYSKIENVLTDASAPRPAEFVFGPEPAHTWCYYYQRAELALQEGAWEEVIEIGNEV
                     NQLGLQPNDRIEWAPFLQAYAIVGDVEAFETTTKKIERSPFIRREACNNLLSTQEMGA
                     VFAPEIQSLMDERVCRGQEKSLP"
BASE COUNT          477 a          536 c          565 g          580 t
ORIGIN      
        1 acaccatgga tcgcctgatc ggtggaccgg gggttttcca ggaaatgttc aggatcgatc
       61 gcccggcccg gggaccactg tttgaagctt attatcaaat atttggtatc cagccatttc
      121 cctaccatat gagttcgttt ctttggcgcg tcgcgggtgg cctggcggct ttatggcttt
      181 tcggtcagct ctggcctcgt caacgcctgg cgacgtttat catggcggtt ttatttgcac
      241 tctacccggg ctatctccgg tggatggaag gcttcgagaa tcaaccgcga atcctgagct
      301 cgtttttaga agcgctctcc attgccctga ccttgcaggc aatcagaacg ccgcgaacca
      361 ttctgaaaat tctgacgtgg atcggttcca ttcttacagg ctgggcatac attgcattgg
      421 tcgattttgc tttcggtatg gaggtcttcc gattattgtg tgtgttcctg ctggtcaacc
      481 gcgatcagga aaacctcccg tttgtgaaga gaagcatttc ggcaatccga gcctggggaa
      541 ttgcggcttt gatcccgttt ggctttctct tctggagatt tttcctgttc cataatgaac
      601 gccaggcaac cgatgtcgga ttgcaactca gctacctgtt tgcctctccc cttacgactg
      661 gagcgatgtg gttgctgcgg cttgttcaga gtaccttcaa tgtgacggtc ccggcgtggg
      721 gctcgtcgct tttccagagc ttgtttgaga tccctctgcc taattccatg cttggcatat
      781 ggattgcggg tattgtggtt gttctctttc tagggatcag tttttcaata gagaagatgg
      841 ataataatcc aacacaattg gatgtggttg cgagccagac ctggcaggta gaagcgatct
      901 gggtcgggct ggcaggagtt gttgcagggg tcatgccggt gattgttgcc aatcggtatg
      961 tatccgtgga ggcctattcg cactatgctt tgccagcgtc tttggccagt gtgatgatag
     1021 tagtgggaat cctgtctttg atcaatctcc gccctgttcg cttaggtgct gcagcattgc
     1081 ttgtcatcct ggcggtgctc acccactatt cagtttcgct gcgtatcctg catgaagagt
     1141 cggtgatcgc acaattctgg cagcaagttg tctggaggtc cccgggcctt aaagccggaa
     1201 ctaccttgtt tgtcagttat ccatccgttt cttacggtga agatgtggac gcagtcgcgg
     1261 gacccgccaa ctttctgtat ttccccgaac aaacgaatca gatccccgct gtctatcccc
     1321 tcatcgcgct tccccagatg gattacacca ctatttatgt gcttcgagga ggccaggaga
     1381 gagcttccgg ctaccggact cacgtgggcg agatcgatta tgacaatatg ctggtgatca
     1441 gccagcccac tgaagatgcc tgcgtccatg tcatcgattc ccaatggccc agatattcga
     1501 acgaggattc ggctcaaatc cttctccttg ggcaatattc gaaaatcgag aacgtactga
     1561 ctgatgcaag cgcgcctcgc ccggcagagt tcgtttttgg gccagaaccg gcccatacgt
     1621 ggtgttatta ctaccagcgt gcagaacttg ctcttcagga aggggcttgg gaagaggtca
     1681 ttgaaattgg gaatgaagta aatcaattgg ggctccaacc caacgatcga atagagtggg
     1741 caccttttct gcaagcctat gctatagtag gcgatgtgga agcttttgaa acaaccacta
     1801 aaaaaataga aagatcacca tttataagaa gagaagcctg caacaatctg ctcagtacgc
     1861 aggagatggg tgctgtattt gcgcccgaaa ttcaatctct aatggatgag agggtctgtc
     1921 gaggtcagga aaagtcactc ccataggccg agacgctctg tcccccactt cttgcaactc
     1981 ctaccagtga ttacgatcgt tctacgcata aggaaggctg catggcaacc ctgatcaaaa
     2041 gcggtactct tattacagca tccgaaacct ttgaagccga tatcctgatt gaaggggaaa
     2101 caatttcaca aattggaaaa agtctccagc acccgaacgc ggaggtgatc gacgcgac
//
LOCUS       DASTJP010000314         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_969431, whole genome shotgun sequence.
ACCESSION   DASTJP010000314 DASTJP010000000
VERSION     DASTJP010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969431"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VFQ23_09370"
     CDS             <1..898
                     /locus_tag="VFQ23_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9906840.1"
                     /translation="RVTAIAARDPKRAQAFARRHKIPHVHQTYEELITDPGIDAIYNP
                     LPNGLHAEWTIRALQAGKHVLCEKPMASNAREAEAMARTAHETGLVLSEAFAYRYHPL
                     TSHVKKILNGGEVGTIKHIEAQFCFLLPFTNNIRFNFELAGGALMDCGCYPVSLIRYV
                     AGSEPVVERAQASLFAPQVDHKMTADLSFADGCTAHLECAMLSPKLFRSFLNVEGDGG
                     QLRIISPFQPHWFHWLTVKGRKGNRSERVQGENSYTLQLRAFIQAIRGEIKLNTDPAD
                     AIGNMRVIDSIYEKAGLKQRGF"
     gene            complement(922..1761)
                     /locus_tag="VFQ23_09375"
     CDS             complement(922..1761)
                     /locus_tag="VFQ23_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9906841.1"
                     /translation="MLISIRNWLGDRPIPAFFFLTFFIAWSIWLTVGFLAPGYFILTI
                     IPGAWAPSISAILLAYLSDGNSGIRQLLNGLLKWRVGFQWYLIVFFGIALIAYAAMGI
                     NILFGGHPGQISLPGGAPFSAWPVIVPLVFFVNIFLGGPLAEDIGWRGYILPKLRQRM
                     SALNASLLIGVVWVLWHIPFYIIPEGRVVVGDVPLLWFAPLTIAWSVLFAWVYVNTES
                     ILMPVLFHAAINTTLGTLGILGPPTGNLMPLVLNTLLTWMAVGVVVVLFGKDLKRNKV
                     AGN"
     gene            complement(1927..2124)
                     /locus_tag="VFQ23_09380"
     CDS             complement(1927..2124)
                     /locus_tag="VFQ23_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906842.1"
                     /translation="MNTKNLINQTAVIFAILAIAGVSLAVGSKFVANPSEQTILVGIG
                     SAMFGAALTFFLIRILSIAGK"
     gene            complement(2181..>2444)
                     /locus_tag="VFQ23_09385"
     CDS             complement(2181..>2444)
                     /locus_tag="VFQ23_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906843.1"
                     /translation="DPELQAGRKKQVESGNALLEGITGAMQQGMVQGLLRSDMDPADL
                     ARAFIAFENGVIQLWLVSPRSFSLKTSAESFAEILLDGLGVQA"
BASE COUNT          679 a          643 c          614 g          508 t
ORIGIN      
        1 aagagtcacc gccattgcag cgcgcgaccc gaaacgcgcg caagcatttg cgcgcagaca
       61 taaaattcca catgttcatc aaacctatga ggaattaatc accgaccccg ggatcgacgc
      121 gatctacaat cccctgccaa acggcctcca cgcggaatgg accatccgcg ccctgcaggc
      181 tggcaaacac gtgctctgtg aaaaaccgat ggcgtccaac gcacgggaag cggaggcaat
      241 ggcaagaact gcgcatgaaa cagggctcgt actcagcgag gcgttcgcct atcgttatca
      301 tccactcacc tcccacgtca aaaaaatcct caatggaggc gaagtaggaa cgatcaaaca
      361 tatcgaagcg cagttttgtt ttttacttcc atttacgaac aacatccgtt tcaactttga
      421 actggcgggc ggcgccctga tggactgcgg ttgttatccg gtcagcctga tccgctacgt
      481 cgcagggtcg gaaccggttg ttgagcgcgc acaggcgagc ctttttgcgc cacaggtgga
      541 ccataaaatg acggccgacc tatcgtttgc agacgggtgc accgcccacc tggaatgcgc
      601 catgctctcg ccgaaattgt ttcgttcctt cctgaacgtc gaaggagatg gtggacagtt
      661 acggatcatc agtccgtttc agccacactg gttccactgg ctgactgtga aggggaggaa
      721 aggcaaccgc agtgaacgtg tacaaggtga aaattcctac accctgcaat tgcgcgcctt
      781 cattcaggcc atccgggggg aaataaaact taacacagat cccgcggatg caatcggcaa
      841 tatgcgcgtg atcgattcca tctacgaaaa agccggatta aaacaacggg gattctgagt
      901 gaagcaagta gattgaggca ttcaattacc tgctactttg tttcgtttca aatctttccc
      961 gaagagtacc accacaactc caactgccat ccaggtaagt aaagtgttca agaccaaagg
     1021 catcagattg ccagtgggcg gccccagtat tccgagtgta ccgagtgtag tgttgatcgc
     1081 ggcatggaat aatacgggca tcaaaatgct ctctgtgttg acgtagaccc atgcaaacaa
     1141 aactgaccag gcgatcgtca gaggtgcgaa ccagaggagc gggacatctc ccaccacaac
     1201 gcgtccctcc ggaatgatat aaaaggggat atgccataat acccacacga cgccgatcag
     1261 caggcttgca ttcaaggcac tcatgcgttg gcgaagtttg ggcagaatgt agccccgcca
     1321 gccaatatct tcagccaggg gtccgcccag gaaaatattg acaaaaaaca cgagaggcac
     1381 aatgacaggc caggcagaga aaggtgcgcc gccaggcagg gagatttgtc ccggatgccc
     1441 gccaaagagg atattgatgc ccatcgcggc atacgcgatg agcgcgatcc cgaagaagac
     1501 aatcaaatac cattggaatc ccacccgcca tttcaacaac ccgttgagaa gttgtcggat
     1561 accagaattt ccatcagaaa gataagcaag caggattgcc gaaatgcttg gcgcccaggc
     1621 accggggatg atcgttagga tgaaatatcc cggagcgagg aacccaaccg tcaaccagat
     1681 ggaccaggcg atgaaaaaag tcaggaagaa gaaggcggga atcggcctgt ccccaagcca
     1741 gtttcttata gagatcaaca tatcgaactc cgagaagata cctgcctcta ttccctccta
     1801 atagagaaat caaatgaagt tcggacaatg attagggtcg cccaagcccg aaaaaaaatg
     1861 ctggattgta cgaatatcaa atgatttgtc cataagggag gaatagaggc aggtagtgtt
     1921 cctgatttac ttccctgcga tggacagaat ccttatcagg aaaaaggtca gagcagcacc
     1981 gaacatggca ctgccgatgc cgaccaggat cgtctgctca gaaggatttg ccacgaattt
     2041 tgagccgact gccaggctga cgccggcaat tgccagtatt gcgaagataa cagctgtttg
     2101 attgatcagg tttttggtat tcattttgtc tccttttgtt catttacata ctcaaaagta
     2161 tgtaaatttg gtaaaaaaat ttatgcttga actccaagac catcaagcaa tatctctgca
     2221 aatgattcgg cactggtctt cagcgaaaag gatctgggtg acacgagcca tagctggatc
     2281 acaccatttt cgaaggcgat aaatgcacgc gccagatcgg ccggatccat gtcactgcgt
     2341 agaaggccct gtaccatccc ctgctgcatt gcaccggtga taccctccag cagggcattc
     2401 cccgactcca cctgcttctt gcgtccagcc tgcagctcgg ggtc
//
LOCUS       DASTJP010000315         7153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1461141, whole genome shotgun sequence.
ACCESSION   DASTJP010000315 DASTJP010000000
VERSION     DASTJP010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461141"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFQ23_09390"
     CDS             complement(<1..488)
                     /locus_tag="VFQ23_09390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9906844.1"
                     /translation="MFKNLEQKNKPFFVFWGLVFVGIIGITDFLTGADYGCSIFNVIP
                     ILLVTWYTNRQCGLAVSILSAFMWLITRIVLSPPHIPFVALLWNTFIRLSFFLVITFL
                     SASLKNSLKQARTDYLTSAINSRYFYDRIQLEIDRLQRYKHPFTIAYLDVDNFKKIND
                     RYG"
     gene            complement(842..2266)
                     /locus_tag="VFQ23_09395"
     CDS             complement(842..2266)
                     /locus_tag="VFQ23_09395"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HET9906845.1"
                     /translation="MSFPEPIPPSPRALLIHFFRRIIKPVTRERRAEVQIQLRDSSDP
                     DFDFFLLVVLSCVIATLGLLSDSPAVIIGAMLVAPLMSPIIGFGLAALTGDDKLLRNA
                     GIALARGAIAAVFISALLTMINIRLPFLTLQELPNEVLARTHPSPIDLTIAFAGGLAA
                     AFALAMPSISAALPGVAIATALMPPLCTVGVGIAMRRWDVAGGALLLFITNAVTIAFA
                     AMLVFSAVGFRVRSENGHLVPRALSVAAFSTLILFIPLSWLSFQFFRTANDNRLINLV
                     VSEAVADFDAELTNLTITDRDDVLHLSITIRTSEALRYEDVDQMQHDIAVQLQRPVSV
                     VVNQVFVARLDPLIPPTLTLTPTFTSTPTPVTLTATLTFTSTITPTTSPSATPIPPTA
                     TATPSLARITNTSGRTLNLLQTPGGPSIGTLRNGDYITVLYESEVVDGLVWLEVIDED
                     GRLGWIPQIYLSLVTLTPTITTTP"
     gene            complement(2903..3946)
                     /locus_tag="VFQ23_09400"
     CDS             complement(2903..3946)
                     /locus_tag="VFQ23_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906846.1"
                     /translation="MKRQFGTYFPVVVIFILTACTASNPQEPSATPTPITEILPTPFS
                     VPEILPTPSSIAPFEKRLLATIDVKYPDELVFVQGFIWVKTDDGHVIQVDPVTNRVVG
                     DIKVDTTNDPYHYCQGLSTDGENIWACSASGDENNHTIEVVRIDPGEKSVVETVKVGK
                     IFDQFNMPLLLNRIWVLSGNGDRLVGIDIATNQPGPAIDLGARCLQVEAVGKILLATC
                     RLDNLLLRIDPVKMEVTDRLTFSNPLNIAATEDGIWLSQTNAVTRLDTESLQPVAIFT
                     RLFNADIFATKEAVWVRLENGFLYRIDPASNQLVEQIKTDQSLSMGSILITPDAIWTT
                     AGDEDLLIRLSLN"
     gene            complement(4133..4765)
                     /locus_tag="VFQ23_09405"
     CDS             complement(4133..4765)
                     /locus_tag="VFQ23_09405"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9906847.1"
                     /translation="MALIIFDYDGVLADTLEDMLQFGQAACNQLGVKHIVTKDDLTKL
                     EVMSFPTYGRACEVPEPLVDEFVRLCLDSFAQKHSPPAIFTDLEPVLRHLSMNHKIAI
                     VTTNSSQNVHAFLVTHRLDGLVAAVYGVDTPGSKAKKITMARDLLLESGRHESVFMVG
                     DSLSDVLAAKEASTASIAVTWGHQSLEILLGGNPDHVVSSPQDLIEVIEQ"
     gene            complement(5007..5651)
                     /locus_tag="VFQ23_09410"
     CDS             complement(5007..5651)
                     /locus_tag="VFQ23_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HET9906848.1"
                     /translation="MRHIDMQTWSRRNHFKLFSSFDHPHFSMCVNMDVTAFYPFVKRN
                     GYSLTVSMVYLIARTSNAIPEFRQRIRGDQVVEHEIVNPGFSILIDEDRFSFCDVEYA
                     EDFSEFAKRAAKKIADVKEHLHIENDPEKDDVLYMSPIPWVSFTSFNHPMQLHPADSI
                     PRFAWGKFFQESDTLKMPLNVQGHHAVMDGIHMGRFYETVQDYLHHPEVVLGAV"
     gene            complement(5886..6464)
                     /locus_tag="VFQ23_09415"
     CDS             complement(5886..6464)
                     /locus_tag="VFQ23_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9906849.1"
                     /translation="MQNSRYDPIEKHLAPEWFTERVYIRDCRIQDVSLLTEIFNACNY
                     VEPLDPTFYIVDENEIKLLVEKSLSETDENKGFRLQCLELKDTGKMIGYFHLQHYSPR
                     LPQRATAFISMFVIHPDFQGKQFAQEIVEGISRQLAECGYMAIWLAVYLKNWSAIRFW
                     IQQGFNKIIEYDGATQFSETAQASLTLEKRLR"
     gene            complement(6519..6737)
                     /locus_tag="VFQ23_09420"
     CDS             complement(6519..6737)
                     /locus_tag="VFQ23_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906850.1"
                     /translation="MMSKTLLNKSAPEPRQSTPRLEWWIAARKDGVRVFEQVVRLEVV
                     SGKCVLSRPGRAPGGCPIPPTSTSNGHT"
     gene            complement(6734..>7153)
                     /locus_tag="VFQ23_09425"
     CDS             complement(6734..>7153)
                     /locus_tag="VFQ23_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906851.1"
                     /translation="GVFLALQQLGAHLVVTGLTSLALLLNGIGSLWAGFFPLPDPRHA
                     ANPFAVGIFLFPILLLFALWKLSYATSIKIYLVITNLLFFGLIPVMSGASGIDTQGYQ
                     GLLQRVIALVFFLPISVGSYFLLEQTKHFPFSKGKKK"
BASE COUNT         1938 a         1679 c         1786 g         1750 t
ORIGIN      
        1 ccataccgat catttatttt tttgaaattg tccacgtcaa gataagcgat tgtaaaagga
       61 tgtttatagc gctggagacg gtcaatttct aattgaattc tgtcataaaa gtaccgtgaa
      121 tttatggcag atgtcaaata atctgtgcgt gcttgcttta atgaattctt caaagaagcg
      181 gagagaaagg taataacaag aaaaaacgat aatcggataa aggtgttcca gagtaatgca
      241 acaaaaggaa tatggggagg tgacagaacg atccgagtaa taagccacat gaatgcactg
      301 agaatggaaa ctgcaaggcc acattgacga tttgtatacc aggtgactaa gagaatcgga
      361 ataacattaa aaatggagca tccataatca gcacccgtca gaaaatcagt aatacctatt
      421 atgccaacaa acacaagccc ccaaaaaaca aagaacggtt tatttttttg ttcaaggttt
      481 ttgaacattt tacgttttct ggtgatctgc ttctcccaat attccctatg tgggcgaacg
      541 gtgtgcgtta ctttcaagtg tgctggcatt gcctcgagtt gggtccagta taaaccgaaa
      601 gcagggaaaa aggtcgtaaa cgctgcagat caggctgcga actttagccg aatggacgct
      661 gggtatccgc atttgccagg tgtatatgtt gttgggcagg cctggtgagc gcaagactcc
      721 aagcccgaaa accaaaacta tgcctgcctt ccttgaaagc gatatccaac ggtgcttcgc
      781 caggtactaa tgcggaaatt ttaggttggc gaagtttcct tcgtctacct ctgtgatccg
      841 atcagggcgt ggttgtgatg gtgggagtca gcgttaccaa ggaaagatag atctgcggaa
      901 tccagccaag acgtccgtcc tcatcaatga cttccagcca gaccaagcca tccaccactt
      961 cactttcata cagcacggtg atgtagtcac cattgcggag cgtaccaatg gaaggtccac
     1021 caggggtctg aagtaaattg agcgttcgtc cacttgtgtt cgtgatgcgt gccaaggacg
     1081 gcgtagcggt ggcggttgga ggaatgggtg tggcgctcgg tgaagtagtg ggcgtgatgg
     1141 tggaggtgaa ggtcagggtc gcggtcagcg taacgggcgt cggcgtcgat gtgaaggtgg
     1201 gagtgagagt cagagtcggt gggatgagcg ggtccaacct ggcgacgaag acctgattca
     1261 cgacaacaga cacgggtcgc tggagctgta cggcgatatc atgttgcatt tgatcgacat
     1321 cttcataacg cagggcttcg gaggttcgga tggtgatgga caggtgcaac acatcgtcgc
     1381 gatcagtaat ggtcaggttt gtgagttcgg cgtcgaagtc cgcgaccgct tcggatacaa
     1441 ccaaattgat cagccgatta tcgttggcag tacgaaagaa ttggaagctc aaccacgaaa
     1501 gcggaatgaa caggatcagg gttgagaagg ccgccacaga cagagcccgc gggaccaaat
     1561 gcccgttctc acttcggacc cgaaagccca ccgcggaaaa aacgagcatg gcagcgaagg
     1621 caatggtgac agcattagtg ataaataaca acagcgcgcc gccggccaca tcccagcgcc
     1681 gcatggcgat gccgacaccc acggtgcaca atggcggcat taatgctgtg gcgatggcca
     1741 ccccgggtaa tgcagcggag atggatggca tggccaacgc aaaggcggcc gccaggccac
     1801 cggcgaaagc aatagtcagg tcaattggcg agggatgagt gcgcgccagc acttcattgg
     1861 ggagttcctg cagtgtgaga aaaggcagcc ggatatttat cattgtgagc aaggcagaaa
     1921 taaacaccgc cgcgatcgca ccgcgcgcca atgcaatacc ggcattacgc aaaagcttgt
     1981 cgtcaccggt caacgccgcc agcccaaacc caatgatggg cgacatgagc ggcgcgacta
     2041 acattgcgcc gatgatgacg gcgggtgagt cggacaatag gccgagcgtg gcgattacgc
     2101 acgatagcac aaccagcagg aagaaatcaa aatcgggatc ggatgagtcg cggagttgaa
     2161 tctggacttc agcgcggcgt tcgcgcgtga ccggcttgat gatgcgccga aagaagtgga
     2221 tcaacagcgc acgcggagaa ggcggaatgg gttcaggaaa actcatagtt ttattctacc
     2281 tgatagcatc gtgacctgct ttaccagggt cagattgacc accttcaact ttgtcagttt
     2341 cacgatctga tgaccagcct tatgcactta ctgcgttggg agatggtgga ggaagcgcaa
     2401 tgcgcgctga accctgatcc atactgaacg cagcgcttac aggaaacacc gaattgcctg
     2461 caccaaaaaa tgctattgag ccttgacagg cataattcgg ctcggtatct gaaccggccg
     2521 aaccgctttt tttatttctc tttcctctct tgatcttgat gcttcttctc tcttgatgct
     2581 gcggttaagc ggtagaccgg tgtttaaggt catccttcaa gacctttccg cgctctcact
     2641 ctgggatagc gtgaagtcga agtcgcccgg cgaccggcca tattgggtca gcaaggcggc
     2701 agactcgctg cgatgttgcg tggcaggcag gttgagatca agcaggatgt gccatacgac
     2761 gtgctcgcgg atcgcaccgg gtatcacata gcggattttg ctgttcaggc ttcttcagcc
     2821 aatacgtcga gaaaactcca catctcccgt ttttcgatca accagcgttc ctgcagagcg
     2881 gcgagtgttg ggtgcgcttg catcaattaa gactgagtcg aatgagcagg tcttcatcgc
     2941 ccgcggtggt ccagatggcg tcgggagtga taagaatact acccatgctc aggctttggt
     3001 cagtttttat ctgttcaacc agttgattgc tggcagggtc aattcggtac aagaagccgt
     3061 tttccagtcg gacccaaacc gcttccttcg tcgcaaaaat gtcagcatta aacagcctgg
     3121 taaatatggc aacaggctgt agcgattccg tatccagtcg tgtaactgcg ttcgtctgac
     3181 taagccatat gccgtcctcg gtggcggcga tgttaagagg gtttgagaat gtcagacgat
     3241 cagtgacttc catttttacc ggatcaatac gaagcaacag gttatccagc ctgcatgtag
     3301 ccaataatat cttgcccacc gcctctactt gcaaacaacg agcgccgaga tcaattgcag
     3361 ggcctggctg attggtggcg atgtctattc ccaccaatct gtcgccattg cccgagagca
     3421 cccaaattct gttgagaagc aaaggcatat tgaactggtc aaaaatcttt ccaactttga
     3481 ccgtttcaac aacgctcttt tcgcctggat caatacggac aacttcgatt gtatgattgt
     3541 tttcatcacc gctggctgaa caggcccaga tattttctcc atctgtgctc agtccctgac
     3601 aatagtgata cggatcgttg gtagtgtcaa ctttgatatc tccaaccacg cgatttgtga
     3661 ctgggtcaac ctgaatcaca tgtccatcat ccgtcttgac ccagataaaa ccctgaacaa
     3721 agacaagttc atcgggatac ttaacatcaa tggttgccag taagcgtttt tcgaagggcg
     3781 caattgaaga gggggttggt agaatctctg gaactgaaaa tggcgtgggc agaatttctg
     3841 tgattggagt cggcgtggca gatggttctt gaggattcga tgctgtacag gcagtcaata
     3901 tgaatataac cacaacagga aaataagttc caaattgacg tttcaaatcg ctaaattctc
     3961 caaccaacga taagatagct ggcgcaaatc cattcttctt ttgttccgca gtcgaaggta
     4021 gtacccaacg gtttgggtct gagaaacgtg gcgctgggca tgccggccgt cggcttcacg
     4081 catagttagg cggcttttct gaatgttagc ataaaaagcc cctcatctta tattattgct
     4141 caataacttc aataagatcc tgtggagaac tgactacatg atcgggattg ccgccaagca
     4201 aaatctccaa actctgatgt ccccatgtaa cggcaatact ggcagtggat gcctcttttg
     4261 cagcaagaac gtcacttaat gagtcaccga ccataaacac tgattcatgc cttccacttt
     4321 ctaaaagcag atcccgcgcc atggttattt tttttgcctt ggaaccaggc gtatcgactc
     4381 catatactgc agcaacgaga ccgtccaggc gatgcgtaac aagaaaagca tgtacgtttt
     4441 gcgaagaatt ggtggtaaca atagcaattt tgtgattcat ggaaaggtgt ctaagaacag
     4501 gctccaaatc agtgaagatg gcaggcggcg agtgcttttg ggcgaacgag tccaaacaaa
     4561 gtctgacaaa ctcatcgacc agaggttcag gaacttcaca agcccgccca taggttggaa
     4621 aagacatcac ttcaagcttg gttagatcat cttttgtgac aatatgtttt acgccgagtt
     4681 ggttgcatgc tgcttgccca aactgaagca tatcctcaag agtatcggcg agcacgccat
     4741 cataatcaaa gatgattaat gccatgaatg gtaatccgtt aaatatttcc cgaaatgatt
     4801 atgcatggaa ctgccaccgg atggcgcttg cgtagcaccc tgtaaaggta ccggcctggg
     4861 gcgagacgag acaacatcag tttgcctaaa cccacttcaa gccacacaaa cttcctcaaa
     4921 acgcgccacg tcccaacctg tcaactgcac gtcgaaaact atcacacaag agacgtgcgc
     4981 agagatggct aagttggaca gtgctcttac acggcaccta gcacaacttc cgggtgatgg
     5041 agatagtcct gcaccgtttc ataaaacctg cccatatgga ttccgtccat gacagcatgg
     5101 tgcccttgta cgttgagcgg catcttcaat gtgtcgctct cctgaaagaa ttttccccac
     5161 gcaaagcgag ggatagaatc cgcggggtgt aactgcattg gatgattaaa gcttgtgaag
     5221 gaaacccagg ggatggggct catgtacaac acatcatcct tttcagggtc gttttctata
     5281 tgcagatgtt ccttcacatc cgcaatcttc tttgccgctc tctttgcaaa ctcggaaaaa
     5341 tcctcagcat actcaacgtc acaaaagctg aaacgatctt cgtcaatcaa aattgaaaaa
     5401 ccggggttca caatctcatg ctctacaact tgatcccctc gaatgcgttg cctgaattcc
     5461 gggatcgcat tcgaagtgcg agcgatgagg tacaccatgg atacagtgag tgaatatcca
     5521 ttgcgcttca caaatggata aaatgcggtc acatccatgt ttacacacat actgaaatgg
     5581 ggatgatcga agctactaaa taatttaaaa tgattgcgtc gagaccatgt ctgcatatca
     5641 atatggcgca tatttcacct cttgctcacc aaatatactt caaatgcatg gttgtatcca
     5701 aagttcgctg cccagcggct cgcggttgca aaacgtggcg ctgcgggtat gggctctgtc
     5761 taagagtaga aaaaaatttt aagccatgct gcgcgaaata attgagagca gattggacga
     5821 gtgagccgag agcgaagatg gctttgttgg acggcttgta aattgcaaac ttaaaagttt
     5881 ttgtttcacc gaagccgctt ttccaatgtc agagaggcct gtgcggtctc ggaaaattga
     5941 gttgctccat catattcaat aatcttatta aaaccctgct gtatccagaa acggatggcg
     6001 ctccaatttt ttaggtaaac ggccagccat atcgccatat aaccgcattc tgccaattgc
     6061 cgggatatcc cttccacgat ttcctgagca aactgttttc cttgaaagtc ggggtgtatc
     6121 acaaacatcg atataaaagc tgtggctcgt tgagggagac gaggggaata atgctgtaaa
     6181 tgaaaataac cgatcatctt tccagtgtcc tttagttcca ggcactggag ccgaaagcct
     6241 ttgttttcat ctgtttcaga aagacttttt tctaccaaga gcttaatttc attctcatca
     6301 acaatataaa atgtaggatc caacggttca acgtaattgc acgcgttgaa tatctcagtg
     6361 agcagtgaaa catcttgtat tctacaatct ctgatataaa cacgctctgt aaaccattcg
     6421 ggggcgaggt gtttttctat ggggtcatat ctactatttt gcataattaa ctttattagt
     6481 ccttcgatgt aattcgcgct gctgtccaac ggcttgtgtt atgtatgccc gtttgaggta
     6541 ctggtgggcg ggattgggca ccctcccggt gctcttccag gacgagacaa cacacattta
     6601 ccggaaacaa cttcaagcct caccacctgc tcaaaaacgc gcacgccatc tttacgggcg
     6661 gcaatccacc actccagcct cggagtactt tgtcggggtt cgggtgcaga cttgttaagc
     6721 agtgtttttg acatcatttt tttttacctt ttgaaaacgg aaaatgtttc gtctgctcca
     6781 aaagaaaata cgagcctaca ctgatcggca ggaaaaagac cagtgcaatg actctttgca
     6841 gaagtccttg ataaccttgt gtgtcaatcc ctgatgcgcc actcatgact ggaattagcc
     6901 caaagaacaa aagatttgtg atgaccaggt atatttttat ggacgtggca taactgagtt
     6961 tccatagggc aaacagcaaa agaattggga acagaaaaat acccacggcg aaggggttgg
     7021 ctgcatgtct tgggtctggc aacggaaaaa atccagccca gagacttccg atcccattca
     7081 gaagcagcgc aagagaagtg agcccggtta caacaagatg tgcacctagt tgttgaaggg
     7141 ctaaaaaaac acc
//
LOCUS       DASTJP010000316         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_396024, whole genome shotgun sequence.
ACCESSION   DASTJP010000316 DASTJP010000000
VERSION     DASTJP010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396024"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VFQ23_09430"
     CDS             <1..1432
                     /locus_tag="VFQ23_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9906852.1"
                     /translation="WTMRMFAGFGTAEETNARFKYLLDQGQTGLSIAFDLATLMGYDT
                     DQPEALGEFGKCGVAVSSLKDMEILLDGIPLEKVSTSMTINSPAAITWAMYLATAENR
                     GVPLDKLRGTIQNDILKEFIAQKEFIFPPEPSMRLVVDTMEFGAQNVPQWNTISISGY
                     HIREAGSTAAQELAFTLGDGLEYVRWGIERGMDVDDFAPRLSFFFNAHNDFFEEIAKY
                     RAARRIWAREMKETFKAKNPRSWLLRFHTQTAGVSLTAQQPENNVVRVAIQALAAVLG
                     GTQSLHTNSLDEALALPSEHAVTIALRTQQIIAEESGVANTVDPLGGSFFVEAMTDRI
                     EQEAYDYFRRVEELGGVIPAIERGFFQSEISDAAYRYQREIDQGIRKIVGVNAYAEKK
                     PLMVPILEMDPQGYNRQLNRLAELRKTRDNGRVGQTLDNLRIAMQGTENTMPYLMECV
                     HAYATLGEIIQVMKEVFGVYEEPTMI"
     gene            1597..>2205
                     /locus_tag="VFQ23_09435"
     CDS             1597..>2205
                     /locus_tag="VFQ23_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9906853.1"
                     /translation="MNDDSRSRKKLQVIINPAAGVGRPILQSLNNGFKDSGIEWDVSI
                     THKAGDAERLAKKALEEGADMVGVHGGDGSVMEVASALRGTNIPLAIFPGGTANVMSV
                     ELAIPSTDLDEAIDFIAKGNYTTRLIDMGRVNDRLFLLRVGIGLEADMMKSADQEVKN
                     RFGMLAYAFSALNEMRNLTPTLYRVRVDGELHEIEGVSCMIAN"
BASE COUNT          567 a          532 c          602 g          504 t
ORIGIN      
        1 ctggaccatg cgcatgttcg cgggctttgg cacggcagag gaaacgaacg cgcgcttcaa
       61 atatttgctg gaccagggac agacgggatt atccatcgct tttgatctgg cgacattgat
      121 gggctacgac actgaccagc ccgaggcctt gggagagttt gggaaatgtg gcgtggcggt
      181 ctcctcattg aaagatatgg aaatcctgct ggatggcatt cccctcgaga aagtatccac
      241 gagcatgacg atcaattctc cggctgcgat cacctgggca atgtacctgg ctacggcaga
      301 gaatagaggc gttccgctcg acaagctacg cggcacgatc caaaacgata tcctcaagga
      361 attcatcgca caaaaagagt ttattttccc gccagagcca tccatgcggc tggtcgtcga
      421 cacgatggaa ttcggcgcgc agaacgtacc ccagtggaat acgatctcga tttcagggta
      481 tcacatccgc gaggcaggtt cgacggctgc acaggaactg gcattcacgc tcggtgatgg
      541 actcgaatat gtgcgctggg gtatcgagcg cggcatggac gtggatgact tcgcgccgcg
      601 cctctccttc ttcttcaacg cacacaatga cttcttcgaa gagattgcca agtaccgcgc
      661 ggcacgtcgc atctgggccc gggagatgaa ggaaaccttc aaggcaaaga atccacgctc
      721 ctggttgtta agatttcata cccaaaccgc cggtgtttca cttacagccc aacagcctga
      781 aaacaacgtc gtgcgtgttg cgatccaggc tcttgccgcc gttctcggcg gtacacaaag
      841 tttacataca aattcgctgg atgaagccct ggccctgccc tctgaacacg cagtgacaat
      901 tgccctgcgt acacagcaga tcattgcaga agaatccgga gtagccaaca cagtcgaccc
      961 gctcggaggc agtttcttcg tcgaggccat gacggaccga atcgaacaag aagcgtatga
     1021 ctactttaga agagtcgaag agttaggcgg agtcatcccc gccatcgaaa gaggattctt
     1081 ccaaagcgag atctccgatg ctgcttatcg ttatcagcgc gaaattgatc agggcattcg
     1141 caagattgtg ggtgtcaatg cctatgctga aaagaaacca ctaatggttc caattctgga
     1201 aatggatcct caagggtaca acagacagtt gaatcgatta gccgaacttc gtaagacacg
     1261 tgataacggt cgcgtgggcc agactctgga taacttgcgt atcgccatgc aaggtactga
     1321 gaatacgatg ccgtacctga tggaatgcgt ccatgcctat gccacgctgg gtgagatcat
     1381 acaagtgatg aaggaagtgt ttggggtgta tgaagagccc acgatgatat agacgattca
     1441 agatattcac tgcgaatctc tgtggggcgc gaagaactgt ttggttcatc aacttgtacg
     1501 tccatcaaaa ccacctttcc gcgcatacgt ttaattaatt ctattggtga taaacttatg
     1561 gcgttttaca taccatccaa cccgaaggca atctagatga atgatgactc tcgctcgcga
     1621 aaaaaattac aggtgattat caatccggct gccggggttg ggcgcccgat cttacagtcg
     1681 ttaaataatg ggttcaaaga ctcggggatc gaatgggatg tgagcatcac ccataaagct
     1741 ggggatgcgg aacgactggc gaaaaaagcc ctggaagagg gtgcggatat ggtcggtgtg
     1801 catggagggg atggcagcgt gatggaagtt gcgtccgcgc tgcgcggcac caatatccct
     1861 ctggcaattt tccctggcgg caccgcaaac gtaatgtcgg tggaacttgc tattccgagt
     1921 acagaccttg atgaggcaat tgattttatt gcaaaaggga actacaccac gcgcctgatc
     1981 gatatgggca gggtcaatga tcgcttgttt ttactgcgtg tcggcattgg actggaagcc
     2041 gacatgatga agagtgccga tcaggaagtc aaaaatcgct ttgggatgct ggcatacgct
     2101 ttttctgctt taaatgagat gcgtaatctg acaccaacac tctatagggt gcgcgtcgat
     2161 ggtgaactgc atgagatcga aggtgtaagt tgtatgatcg ccaat
//
LOCUS       DASTJP010000317        11312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1291623, whole genome shotgun sequence.
ACCESSION   DASTJP010000317 DASTJP010000000
VERSION     DASTJP010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291623"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFQ23_09440"
     CDS             complement(<1..184)
                     /locus_tag="VFQ23_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9906854.1"
                     /translation="MAEVSFGEWLKRQRGANGWTQGQLAQQLNCSISMLRKMEETTRR
                     PSVQVIQQLTEIFKIPN"
     gene            332..556
                     /locus_tag="VFQ23_09445"
     CDS             332..556
                     /locus_tag="VFQ23_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9906855.1"
                     /translation="MTNLSDLTHRELEILQLVLEGKTNKAIAQEFYISEKTVEFHLDN
                     IYSKIGARTRLMAGIWAFQNGMDGKNPGNP"
     gene            676..3144
                     /locus_tag="VFQ23_09450"
     CDS             676..3144
                     /locus_tag="VFQ23_09450"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HET9906856.1"
                     /translation="MFKVNLDKFPHVMTIMIVIALVAIQFLGITFLFETSPASNESSE
                     LSGNESSVLANTRNVELLQFTSAGHVLGFEPGGMYVATASHAMRVEFMDVNTVMPSSA
                     DASDEGAVPLTSVTYANLWDGVTLTYDANGIVRSTYRLEPYAEVNTIRLRYSRPAQVQ
                     PDGSLRLEFESGAMTETAPVAWQEIDGQRVDVNVSFRALSENEIGFALGSYNSAQPLF
                     IDPTLTWNTFMGSSGIDFGNAIAADSLGNVYVAGNSTTTWGTPVNAHAGATDAFAAKL
                     DSSGVRLWHTFLGGSGSENGFGIGVDGLGNVYVTGTSTVTWGTPVNAHAGGQDAFAAK
                     LDSNGVRLWNTFIGSTGTDNGRAIAVDSLGNVYVAGNSPVTWGTPVNSFNVGTIDAFA
                     TKLDSNGARLWNTFMGGSGSDQSFGIAVDSLGNVYVAGSSPATWGTPVNSFGGGTNDA
                     FATKLDSSGVRLWNTFMGGSGNDNGNAIAVDAAGGNVYVAGSSNATWGTPVNAFSGGT
                     NDAFAARLDSSGVRTWHTFMGGSGNDQSFGIAVDAIGGNVYVAGSSDATWGTPVNPHA
                     GIFDAFADKLDSSGVRLWHTFLGGVNSDNGRGTAADGLGNVYVGGDSSAPWGVPVNPH
                     TGIGNFDVFAAKIFDVLPPSPTPTDTPTPTPTDTPTPTPTDTPTPTPTDTSTPTPTDT
                     PTPTPTDTPTPTPTDTATPSPTFTFSGFAAPVDNPPTVNTGKAGRTYPVKWQLRDANG
                     NYVSALSAISSITYKSTSCSVFTGDPTDALETIATGGSILRYDSSANQYIYNWATPTT
                     PGCYTLFVTLDTGQVFVAYFNLSK"
     gene            3188..3580
                     /locus_tag="VFQ23_09455"
     CDS             3188..3580
                     /locus_tag="VFQ23_09455"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9906857.1"
                     /translation="MNAQENKEFIRKYIEAISGKPKPSELVDLYVAEQPLIEHIAMNE
                     AAFPEYEMHIEKLIAEDDLVSVIGRAGGTHKGPFMGMPPTGKSWDVPIHITYRIQGGK
                     IVEHWMVIDTAAFMQQLGMIPSAADSTS"
     gene            3937..4437
                     /locus_tag="VFQ23_09460"
     CDS             3937..4437
                     /locus_tag="VFQ23_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906858.1"
                     /translation="MNTFTNSMHPKKVASQASRNRPFQNISGLIIVLALAIVAVTAIN
                     QANRLVAAENSPAIAYSNALEMQYAQPWIDAQNKPAVSFGNALELQYAQPWIDAQNSS
                     VVPFSNALELQYAQPWIDAQNRPAISFGNALELQYGQPWLDAQQSQLNCHSSLEMLYA
                     CQYGRP"
     gene            complement(4558..8436)
                     /locus_tag="VFQ23_09465"
     CDS             complement(4558..8436)
                     /locus_tag="VFQ23_09465"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9906859.1"
                     /translation="MTLSSSTPNSFNSFGDLLKFLRRRQRLTQLELSIAVGYSESQIT
                     RLEKNQRLPDISAIKALFIPALQLENEVELTNYFLELAESARQEDAPAPGIAPYKGLL
                     FFDDFDADLFFGRESLIATLTGHVIDLAAADSALRFLAVVGASGSGKSSLVRAGLAVA
                     LKRAGWDVRVFTPTATPLKILKTSMTAVQGKSSERVLILVDQFEEVFTLCRNETERID
                     FIDQLLSLAQEKSRINTVVIALRADFYSHCAQYPFLRQAVAAEQEYIGQMNREELRRA
                     IEEPAKRGSWEFEPGLVELLLNDIGAQGSQEPEPGALPLLSHALLATWERRRGRLFTM
                     EGYHASGGVRSAIAETAESIFTDQLNQNQQELAHYIFLRLTELGEGTEDTRRRAILNE
                     LMGQPEETIQLRGLLNTLAEARLITLNEDSAEVAHEALIREWSRLREWLTADREGLKL
                     HRHLTESAHEWEALNKDVGALYRGARLAQAREWSEINSVRLSLSEQDFLSASVEQEQR
                     ELLEREALRQRELEATKELAETQRQSALRLRISNRVITAVAGVAVILAVLAGMLGLQS
                     NRNSIAAQNNAARAQAEAETSATQRAIAEENFVRAENQQLAAQANRIISNPTGNSELA
                     ALLSIYSMRNGYIVNADTALAKAMLNLHTQFIIHPELATAGGATFSPDGTMIATGASD
                     QTIELWDSKTGEFLRTLSKGPSGSYSSLPDLTFSPEGNWLFVRNNLGAIRLIDFETGE
                     TAQIFAIPIEHQVSHMAISPDGKMALGARVKSIFVWETLSGKIIRTFFTPDVVFDVKF
                     SPDNQSILVGLTNGDTFLLDLTDGKPLQEYKHSSSRSVVAFSPDSQYVAIGQNNGEIY
                     LHRTSTTELVRTFSGHQNAVLSLAFSPDGQSLISSSWDTTVRLWDVSTGQQLNIFSGH
                     TRRVVKVEFSPDGTSALSSAEDGDVRVWRIRANTLDEHEPSARFMPNVRVVRSSDGQV
                     MLVERNDEVNQTAMLNFIETETNKIFFSMEVPRLSYRRLSSDGKYILLNIAGESTYVT
                     QIWDIQAGKLTLELNRNWAIFNAIFSPDNSAVVVAERDEVRLYLWEIPSGKLRPIWTW
                     GETARGFFGDADSEALAWSPDGRYIAVGTQIGQTDIVDLRLSTRATWFGVPFNTPTTW
                     VTAVAYSAAGDKIITGETSGKIRLWELATAKIVKEFNGHIDAVLSIKFSRDGKYILSA
                     SNDKTLRLWDLNTGKLVRVFHHVESVNYADFSSDGKFIIGSGANGAQLWDVDYQDTIQ
                     AACALLVRDFTPEERAQYDIPDDISTCPTK"
     gene            8856..9404
                     /locus_tag="VFQ23_09470"
     CDS             8856..9404
                     /locus_tag="VFQ23_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906860.1"
                     /translation="MNTLSKTLVMFAVLSLLTSACGGRLVQAESSESTAVPTISYPFL
                     LGKSLSDPDVADFIVSNQCTPADQFQLCKDTGLALWTGADQIVSMVYLYAGNAEGFRR
                     YRGDLPYGITFYDPMWRVQEKLMDPNSANPLYQAGLPDESGTPDHMHFRAVYKQLGMT
                     VMYITPGEDEDAYIYAIVVYKQ"
     gene            9427..9654
                     /locus_tag="VFQ23_09475"
     CDS             9427..9654
                     /locus_tag="VFQ23_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9906861.1"
                     /translation="MTHLSDLTQRELQILQLVIEGKTNKAIAHEIQISQKTVEFHLDN
                     VYSKLGARSRLMAGIWALQHGINMEPRETPS"
     gene            complement(9797..>11312)
                     /locus_tag="VFQ23_09480"
     CDS             complement(9797..>11312)
                     /locus_tag="VFQ23_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9906862.1"
                     /translation="CIDWSWNLLSDSEQIVLSRLTVFAGGFTLEAAAQVCAGEGIESH
                     QVFGFITQLVQKSLVVENRDIGNEQRYRLLETIRQYAREKLASFGQEETIRTRHLHYF
                     LQLSELAEPALKGPAQIEWRVKLNAERDNIRVALTWADKTDVEAGLYLCSRFGRFWES
                     FDIREENDWLSTFLQKPGSQAYQRARAMALYTHLGILNYLNQRDTWRSTADECLELCR
                     ALGNQSIEMDILLMRAGNISNAAQRADFFQTAIKLAQASGDIWRQARTLDQVGWHYVG
                     DERLSYWKQAIKLFRQAGDWRSLAQLLCIAGYFALLNSDLELAQKSLAEAAVLNDQLK
                     DKLIKAEILSAYAQLATISGEYDQAHVYFQAELETWEELGSRLTLLWCRSHLGDLALK
                     EGNFKEARDILTETARAFFNDKTTIGVVFTLEKIANLCVAIGNPETAAQLIGWADATR
                     RKLDDIRPRLEQEDVDKCITACIESMGEIAFSDAYDEGQKMTLEEALVYALKES"
BASE COUNT         2862 a         2887 c         2655 g         2908 t
ORIGIN      
        1 cgttgggaat tttaaatatc tcagtcaatt gttgaatgac ctgcacggaa gggcggcgtg
       61 ttgtctcttc catcttacgg agcatgctta ttgaacaatt gagttgttgg gccaattgtc
      121 cttgtgtcca cccattcgca ccccgttggc gtttgagcca ttctccgaac gatacctcag
      181 ccacagtctc ctccacaatt tgtgtcactg ttgtgtcact gagcgacacc tgcatgtttt
      241 cgattccccc tatagtttaa ccgttgattc aaagtatatc aatctcatca cgcaacttat
      301 tgaatctggg aatacatttg ggaaatggaa aatgacaaac ttatctgatc tcacccatcg
      361 tgaattggaa attcttcaat tggtccttga aggaaagacc aacaaagcaa ttgcccaaga
      421 attttacatt agtgaaaaga cagtcgagtt tcacctcgac aatatctact caaaaatcgg
      481 tgcacgaaca cgcttgatgg cagggatttg ggcttttcag aatggcatgg atggaaaaaa
      541 cccgggaaat ccctaggtag catgttagga gggaggcttc tacaattaaa tgaattggat
      601 tagaaatcat tattgctcaa gacataccgt tgaaaagtgg ctttgttgtt tcatcatgtt
      661 caggaagaaa atattatgtt caaagtaaat cttgataaat ttcctcacgt aatgacgatc
      721 atgatcgtca ttgctctggt ggcaatccag tttctcggga tcactttcct gtttgaaact
      781 tcgccagcaa gcaatgaatc atctgagtta agcgggaacg aaagttctgt tcttgcaaac
      841 acaaggaatg ttgaactact gcaattcacc tccgccggcc atgtgctggg ttttgagcca
      901 gggggaatgt atgttgctac agccagccat gctatgcgcg tggagttcat ggatgtcaat
      961 acagtaatgc catctagcgc ggacgccagc gatgagggtg ctgttcccct aacgagtgtt
     1021 acttacgcta acctgtggga cggtgtgacg ctaacttacg atgcaaacgg catcgtgcgc
     1081 agcacgtatc gcctggaacc atatgcggag gtgaacacaa ttcggctgcg ctacagccgc
     1141 ccagctcagg tgcagccgga cggctcgttg cgtcttgagt ttgaatccgg agcaatgacc
     1201 gagacggcgc cggttgcgtg gcaggaaatt gatggacagc gtgtagatgt gaatgtaagc
     1261 ttccgggcct tgagcgaaaa tgaaattggc tttgcccttg gcagttacaa ctctgcccag
     1321 ccacttttta ttgatcccac cctaacctgg aacaccttta tgggctcgag tggtattgac
     1381 tttggcaacg ccatcgccgc ggacagtttg ggcaacgtct acgtcgcagg gaacagcacc
     1441 accacctggg gcacgcccgt caacgcccat gcgggtgcga ccgatgcttt cgccgccaag
     1501 ttggacagca gcggtgtgcg gctgtggcat acctttcttg gcgggagtgg tagtgaaaac
     1561 ggctttggta ttggcgtgga cggtctgggc aatgtctacg tgacaggaac aagcactgtt
     1621 acctggggca cgcccgtcaa cgcccatgcg ggaggacaag atgcgttcgc tgccaagctg
     1681 gatagcaacg gtgtcaggct gtggaacacc tttatagggt cgaccggcac tgacaatggc
     1741 cgcgccatcg ccgtagacag cctgggcaat gtctacgtgg cggggaacag ccctgttacc
     1801 tggggcacac ccgtcaactc cttcaatgtg ggaacgattg atgccttcgc caccaagctg
     1861 gatagcaacg gtgcacgtct atggaacacc tttatgggcg ggagcggcag tgaccagagc
     1921 ttcggaattg ccgtcgacag cttgggcaat gtctatgtgg cgggaagcag tcccgccacc
     1981 tggggcacac ccgtcaactc ctttggaggt ggcacaaacg atgccttcgc taccaagctg
     2041 gatagtagcg gtgtacggct gtggaacact tttatgggcg ggagcggcaa tgacaatggc
     2101 aacgccatcg ctgtggacgc tgccgggggc aatgtctacg tggcgggaag cagcaacgcc
     2161 acctggggca cacccgtcaa tgccttcagt ggcggaacaa atgatgcctt cgcggctagg
     2221 ctggatagca gcggtgtgag gacgtggcat acgtttatgg gtggaagcgg taatgaccag
     2281 agcttcggca tcgctgtgga tgccatagga ggcaatgtct atgtagcggg gtccagtgac
     2341 gccacctggg gcacaccggt caaccctcac gcggggattt ttgatgcctt cgctgacaag
     2401 ttggatagta gtggtgtgcg cctgtggcat acttttctgg gaggagtcaa ttcagataac
     2461 ggccgtggga ccgcggcaga cggcctgggc aatgtctacg tggggggcga tagcagtgcg
     2521 ccttggggcg ttcccgtcaa cccacacaca gggatcggaa atttcgatgt cttcgctgca
     2581 aagatattcg atgtgcttcc gccaagcccg actccgacgg atacacccac tccgacaccg
     2641 acggatacac ccactccgac accgacagat acaccaacgc cgactcccac agatacatcg
     2701 acaccaactc cgacggatac gccgacccct actccaaccg atacgccaac accaactcca
     2761 actgataccg ccacgccgtc gccaaccttc accttcagtg gttttgcagc tcccgtcgac
     2821 aatccgccga cggttaatac ggggaaagct ggcagaacct atccggtcaa gtggcagttg
     2881 agagatgcca acggcaacta tgtaagcgcc ctctcagcga tatcctcgat tacatacaag
     2941 agcacatcgt gtagtgtttt cactggcgat ccaacggatg ccctggagac cattgcaacg
     3001 ggaggtagca tcctgcgtta cgacagctca gcgaatcagt atatctacaa ctgggcaacg
     3061 ccaactactc caggttgtta caccctattc gtgacgctgg atactggaca agtgtttgtt
     3121 gcctacttta acctatccaa gtaaaaccga ttcagttgcc ctcttatcaa taaaaggagt
     3181 aaatctaatg aacgcacagg aaaacaagga attcatccga aagtacatcg aagcgatcag
     3241 cggtaaaccc aagccctcgg aactggtgga tttgtatgtc gccgagcagc ccctgatcga
     3301 gcacattgcc atgaatgagg cggccttccc ggagtacgaa atgcatattg aaaaactgat
     3361 tgcggaagat gacctcgtat ctgtcattgg acgggccgga ggtactcata agggtccctt
     3421 tatgggaatg ccgcccacgg gaaagtcctg ggatgttccg attcacatta catatcggat
     3481 tcaaggtgga aaaattgtgg aacattggat ggtgatcgat acggctgctt ttatgcagca
     3541 gcttggtatg attccaagtg ctgcagattc aacatcctga tgagcctaat aacctaaccc
     3601 tctactggag acaggccgga aggggaaagc tgtcccatag aaaatgcggt cgggaatttt
     3661 tctcgaccgc attttctatt attttccgtc ttttacgacg atcggttggg acggggagct
     3721 atccaaatta aattgcggaa taccgagaaa ctgcaggcca agccgtgggg atatgccact
     3781 ccttttcact ttggctcaac caatacggcg cttatttcta caaagtgggc gaaaaagccc
     3841 actacacgcg ttccgcaacg ctttctgaca cgctgggtca acgcggcctc cgtagaatgt
     3901 ggtcatgttc aaacaagttc aaaaaaggag aatatcatga acacgttcac gaattccatg
     3961 caccccaaga aagtggcctc tcaggccagt cgcaatcgcc cgttccaaaa tatatccgga
     4021 ttaatcatcg tcctggccct tgctattgtc gctgtgactg cgatcaacca ggcgaaccga
     4081 ctcgttgctg ctgagaacag cccggctata gcgtatagca atgctctgga aatgcaatat
     4141 gctcaaccct ggatcgatgc gcaaaacaaa ccagctgttt cattcggaaa tgcgctcgag
     4201 ttgcagtatg ctcaaccctg gattgacgca cagaacagct cggtggtccc atttagcaac
     4261 gctcttgaat tacagtatgc tcaaccatgg attgacgctc agaacagacc cgccatttct
     4321 tttggcaatg cgttggaact acaatacggt cagccctggc tggatgccca gcaaagccag
     4381 ctcaattgcc acagcagcct cgaaatgctc tatgcctgcc aatatggtcg cccataagat
     4441 catcaatgca gaaacatgaa taaaaacaaa accttcaacg aaccgtcact gggagtgcat
     4501 gcctagtggc ggtttgttag tatttgtcca acgccagggc aacggcttga ttcttcatta
     4561 ctttgtagga caagtgctta tatcatcggg gatatcatat tgtgctcgtt cctccggggt
     4621 aaaatcgcgc acaaggagcg cacaggcagc ctgaatagta tcctgataat caacatccca
     4681 tagctgtgca ccgttagcgc ccgagccaat aataaacttt ccgtcagaag aaaaatctgc
     4741 ataattcact gattctacat gatgaaatac gcgtacgagc ttgcctgtgt ttaaatccca
     4801 caaccgtagt gttttgtcat tgctcgctga caaaatatac ttgccgtctc gtgagaactt
     4861 gatggacaat acagcatcta tatggccgtt gaattctttc actatttttg cggttgcaag
     4921 ttcccagagg cgtattttcc cgctggtttc acccgtgatg attttgtcac ccgctgcaga
     4981 ataggccaca gcagttaccc aagtggtggg tgtattgaaa ggcactccaa accatgtagc
     5041 tcgagtactg agtcgtaggt cgactatatc ggtctgcccg atctgtgttc ctaccgctat
     5101 gtagcgtcca tctggtgacc atgctagagc ctcagagtcg gcgtcgccaa aaaaaccacg
     5161 ggccgtctcc ccccaggtcc agataggcct caatttccca gatggaattt cccacagata
     5221 taatctcacc tcatccctct cagcaaccac tacagctgag ttgtctggag aaaatatcgc
     5281 attgaaaatg gcccaattcc tgtttaactc aagggttagt ttgccggctt gtatgtccca
     5341 aatttgcgta acataggtgg actcaccagc tatatttaat aagatatatt ttccatccga
     5401 tgataggcga cgatagctca atctgggtac ctccatcgaa aagaatattt tatttgtttc
     5461 agtttcaata aaattcaaca ttgctgtttg gttgacctcg tcatttcttt cgacaagcat
     5521 tacctgccca tccgatgacc gcactacccg aacatttggc ataaaacgtg cactgggctc
     5581 atgctcatcc agggtgttgg cgcggatacg ccaaacccgt acatctccat cctcggcaga
     5641 tgacaacgct gatgttccat ccggggagaa ttccaccttc acaaccctgc gagtatgccc
     5701 ggaaaatata ttcaactgct ggcctgttga tacgtcccac agcctgacgg tcgtatccca
     5761 actgctggaa atgagcgatt gcccatccgg tgaaaaggcg agactaagca ctgcgttctg
     5821 atggccgctg aatgttctca ccagttctgt agtcgatgtg cgatgtaagt atatctctcc
     5881 attattctgc ccgatcgcga catattggct atccggcgaa aaggctacaa ctgaccttga
     5941 actgctatgc ttatactcct gtaacggttt tccatcggtc aaatccaata gaaatgtatc
     6001 tccatttgtc aaaccaacta aaattgactg gttgtcaggg gaaaatttca cgtcgaacac
     6061 cacgtctggc gtgaaaaaag ttctaatgat tttgccagat aaagtttccc atacgaatat
     6121 actttttact ctggctccaa gagccatttt tccatcagga gaaatcgcca tgtgggaaac
     6181 ctgatgctct atcggaattg caaaaatttg cgcagtttca cctgtttcaa aatcgattaa
     6241 tcgaattgca ccaagattat tccttacgaa caaccagttg ccctctggtg agaaggttag
     6301 atcagggaga ctgctgtagg aaccagacgg gcctttcgag agagtacgga gaaattctcc
     6361 tgttttcgaa tcccaaagtt ctatggtctg atcgcttgcc cctgtagcga tcatcgtgcc
     6421 atctggtgaa aaggtagcgc ctcctgcagt agccagttcg ggatgaatga taaattgagt
     6481 atgtaagttg agcattgcct tcgccagagc tgtatctgca ttaactatgt agccgttcct
     6541 catcgaataa atactcaaaa gtgcagctaa ttcggagttc ccggtgggat tgctaatgat
     6601 gcgattagcc tgtgctgcca gttgctggtt ttctgccctc acgaagtttt cctcagcaat
     6661 tgctctttga gtcgcactag tttctgcttc cgcttgggct ctggctgcat tattttgtgc
     6721 tgcgatagag ttcctgttgg attgcaaccc taacattccc gccagcactg ccaaaataac
     6781 tgcaacacct gccacggcag ttatgacgcg attactaatt cgcaagcgta aggcggattg
     6841 gcgctgagtt tctgccagtt cttttgtggc ttcaagctcc cgctggcgaa gtgcttcacg
     6901 ctccaacagc tcgcgctgtt cctgttcaac cgatgcggaa agaaaatcct gttcagataa
     6961 gctcaaacgc actgaattta tttcagacca ttcgcgtgcc tgggcaagcc gcgcaccccg
     7021 atacagtgcg ccaacgtctt tgttcaaggc ttcccattcg tgagccgact ctgtaagatg
     7081 gcgatgcagc ttcagtcctt cacggtccgc agttagccac tctctcagtc ttgaccactc
     7141 gcggatcaag gcttcatgag cgacctctgc gctatcctcg ttcagtgtga tcaagcgtgc
     7201 ctcggccaat gtgtttaaga gcccgcgcaa ttgaattgtt tcctcaggtt gacccataag
     7261 ttcattcaag atcgcgcggc gacgggtatc ttctgttccc tcacctaatt cagtgagacg
     7321 caggaaaata taatgtgcaa gttcctgctg gttttggttg agttgatcgg tgaagatgct
     7381 ttcagccgtc tcggcaattg cgctacgtac cccacctgaa gcgtgataac cctccattgt
     7441 aaataggcgt cctcgacgac gttcccatgt cgctaataga gcgtgcgata acaatggtaa
     7501 cgctccgggt tccggttcct gcgagccttg tgctccaatg tcatttaaca ataactcaac
     7561 cagtccgggc tcaaattccc aactgccgcg tttagctggt tcttcaatgg cgcgccttaa
     7621 ttcctctcta ttcatttggc cgatatattc ctgctctgcg gcaactgcct gtcgcaaaaa
     7681 gggatactgc gcacagtgcg aatagaagtc agcgcgcagc gcaatgacca cagtgttgat
     7741 tctcgatttt tcctgggcaa gtgaaagtag ttggtcaata aaatcgatac gttcggtttc
     7801 attacgacac aaagtaaaca cttcctcgaa ttgatccacc aggattagaa ctcgttctga
     7861 gctttttccc tgtactgcgg tcatacttgt tttcagaatt ttcagcggcg ttgcggtcgg
     7921 agtgaacacg cgtacatccc agccggcgcg tttcaacgca acagccaatc ctgcgcgtac
     7981 tagtgaagat ttgccgcttc ccgacgcccc aactactgcc agaaagcgca gagccgaatc
     8041 tgctgcagcc aggtctataa cgtgtcctgt aagcgttgct atgagagact cgcgcccaaa
     8101 gaataaatct gcatcaaagt catcgaagaa cagtaatcct ttgtaggggg caatacctgg
     8161 cgcgggtgca tcttcctgtc gtgcagattc ggctagttcc aagaaatagt ttgtaagctc
     8221 cacttcattt tccaactgca gggctggaat aaatagtgcc tttatcgctg aaatatcagg
     8281 caggcgctga tttttttcga gtcgggtaat ttgcgattca ctataaccga cggcgatgga
     8341 gagttccagt tgggtgagcc tttgccggcg gcgcagaaac ttaagcaaat caccaaaaga
     8401 attgaatgaa tttggagtgc ttgaactgag agtcatgttg agtcattttc caatggaatt
     8461 attaggaata cccagctaac catattgtag tcattggctc tatatattca agacccaatc
     8521 gatgaaattc atgttttgtc aggaaatgtc agctttttca gtgaacacgt gtcaattaat
     8581 gtcagatctt acgcaggatc cctaaataag atgtagacac gttcgcaacg ttttcattac
     8641 caacgagaat tcttttgccc cgcttgagtt ttatatcagt ttttgtatca ctttccgcat
     8701 tatgtaaatc acccaatttc agtaaattat acaaagattt attggcttgc caatggatag
     8761 caccgggtca tttagttcca aataattcat tccatatgct ttcgatgcca gaaagatacg
     8821 taatcctccg ttaattgata aaggaagaga atgtcatgaa cacgcttagt aaaaccctcg
     8881 ttatgtttgc tgtgctatcg ctcctgacca gtgcctgcgg tgggagactg gttcaggctg
     8941 agtcatcgga atccacggct gtgcccacga tcagttatcc attcctgctc ggcaaatcgc
     9001 tgagcgatcc ggatgttgct gacttcatcg tcagtaatca gtgcacgcct gcagaccaat
     9061 tccaactgtg caaagataca ggcttggcac tatggacagg tgcggaccag atcgtaagca
     9121 tggtgtatct atacgccggg aacgccgaag gcttcaggcg ctatcgaggt gatttgccct
     9181 atgggattac tttttacgac cctatgtggc gggtgcagga gaaattgatg gatccaaatt
     9241 cagcaaatcc cctgtatcag gctggtctgc cagacgaatc agggacaccc gatcacatgc
     9301 attttcgggc tgtatacaag caactcggta tgactgtgat gtacatcacc cccggtgagg
     9361 acgaagatgc ctatatctac gcgattgtgg tctacaaaca gtagtcaatg aacaacggag
     9421 ctgaaaatga cacatttatc tgatctcaca cagcgtgaat tacaaattct tcaattagtt
     9481 attgaaggca agacgaacaa ggcaattgcg catgagattc aaattagtca gaagacagtt
     9541 gaatttcacc tcgacaacgt ctactcaaag cttggcgcgc gttctcgcct gatggcaggc
     9601 atttgggctt tgcagcatgg catcaatatg gaacctaggg aaactcccag ctagcctgcg
     9661 atgattgagg cttctacaat cgacaaacac ggaatccatt aacctggccc cttgccagag
     9721 acagaccaga aggggaaacc tgtctcatcg aaaacgcggt tgagctatat tctcaaccgc
     9781 gttttgtatt cctgatttaa ctttctttta gcgcgtatac aagcgcttcc tccagggtca
     9841 ttttttgtcc ctcatcatat gcatctgaaa atgcaatctc gcccatcgat tcaatacagg
     9901 ctgttatgca tttatccaca tcttcctgct caaggcgcgg acgtatgtca tccaattttc
     9961 ttcgagtcgc atctgcccag ccgatcaatt gcgcggcagt ctcaggattt ccaattgcaa
    10021 cacataggtt tgcaatcttt tccagagtga atacaactcc tatcgtagtc ttatcattga
    10081 aaaatgctcg tgcagtttct gtcaatatgt cacgtgcttc tttaaagtta ccctctttca
    10141 aggcaagatc tcccaaatgc gagcgacacc agagcaaggt caagcgagat cctaattctt
    10201 cccaagtctc tagctctgcc tgaaagtaga cgtgcgcctg atcatattct cctgatattg
    10261 ttgctaactg tgcataggca gataatattt cagcttttat caatttatcc ttcaactgat
    10321 cgttcaacac cgcggcttca gccaaacttt tttgagccaa ctcgagatca ctattcaaca
    10381 gcgcaaagta tcctgcaatg caaagcaact gggctagcga ccgccaatcc cctgcctgac
    10441 gaaacagttt aatcgcttgt ttccaataag aaagtctttc gtccccaaca taatgccaac
    10501 ccacctgatc gagtgtcctt gcctgccgcc agatatcacc tgaggcctga gctagtttta
    10561 tggccgtttg gaagaagtcg gctctttgtg ctgcatttga gatgttcccg gctcgcatta
    10621 gtaatatatc catctcaatg ctttgatttc caagagcgcg acacaattct aaacactcat
    10681 ccgccgttga ccgccatgta tccctttgat tcaagtaatt caaaatcccc aggtgcgtgt
    10741 acaacgccat tgcccgtgcc ctttgataag cctgtgaccc tggtttctga aggaaggtag
    10801 acagccaatc attttcctcg cggatatcga aactttccca aaagcgccca aacctgctgc
    10861 agagatacaa tccggcttcc acgtcagttt tatctgccca ggtcagggcc acgcgaatgt
    10921 tatctcgttc cgcattcaac ttcaccctcc attcgatttg tgccggacct ttcaaagcag
    10981 gttctgccag ttcagatagc tgcaggaaat aatgtaaatg gcgtgtgcgg atcgtttctt
    11041 cttgaccaaa actggctaac ttctcacgcg catactgacg aatcgtttcc agcagacgat
    11101 aacgttgctc attacctata tcccgattct caactaccag tgatttctgc acaagttgag
    11161 tgatgaaacc gaacacctga tgagactcaa ttccctctcc cgcacacacc tgcgctgcgg
    11221 cttccagcgt gaagccgccc gcaaacacgg taagcctgct taggacaatt tgttctgagt
    11281 cagagagtag attccaactc caatcgatac at
//
LOCUS       DASTJP010000318         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_537073, whole genome shotgun sequence.
ACCESSION   DASTJP010000318 DASTJP010000000
VERSION     DASTJP010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537073"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFQ23_09485"
     CDS             complement(<1..619)
                     /locus_tag="VFQ23_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9906863.1"
                     /translation="MRNKLYYVLSTLLLASVLLSACGPAATETAAPAETQAPVVTDIP
                     TEMPTEAPTEVPTEAPAGPLTVLIDNDEGPITPANFNTFIGFWLVGWVYDSLYIRTPE
                     LEPIPSLATSATPSEDGLTWDVTLREGVKWHDGEDFTVDDVIFSYNFLIAAGRANNLA
                     AIDTMEANGDYGLTIKLKEPQPFFLNEGLAGTFILPEHIWKDQTPV"
     gene            complement(815..1906)
                     /locus_tag="VFQ23_09490"
     CDS             complement(815..1906)
                     /locus_tag="VFQ23_09490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9906864.1"
                     /translation="MKSNPRIAFVIDSLPSLGGAEKVLFTALEIYPQADIFTLIYNKE
                     VFKSTPVAEREIKTSYLDRFPLAHRHHRMFLPLMPAAIERFNLQKYDLIVSFSYAVAH
                     GVHNFNGARHVSYTYTPMRYAWTDINLDGTHTNRNIIVDKLLQAFCRWDRKAAARVHQ
                     FAAVSQAVSRRIQSAYQREAPVIYPPVEVDRFQPNGQREDYYVTVSRLVPHKRIDLLV
                     EAFTELNLPLLIIGDGAELPRLRALAGSNIRFLGYQTDESVIELMSKARGFVCAAEED
                     FGIAIVEAQAAGCPVIVYGRGGALETVLDGTTGVLFSEQTKESLIEAILQFERIHSSF
                     CVDDLVQQAKRFDKTRFIDRFVEFVEPTM"
     gene            complement(1896..2900)
                     /locus_tag="VFQ23_09495"
     CDS             complement(1896..2900)
                     /locus_tag="VFQ23_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9906865.1"
                     /translation="MPPNVVINGRFLARRVTGVERYARELLSHLPTDCSIAKPSLAST
                     GLRGHTWEQFVLPQKLGANSILWSPANTGPLMVSNQALTIHDLSPIEHPEWFQSTFAM
                     WYRMFLPILVKRVRIVFTPSEYVRQKVTSRFGIRNVKVTPNGVDHCRFTLKAKQTKYN
                     LPARYVLFVGSLEPRKNLPALLQAWGEVKSDFQDTWLVIAGASGRVFQPVNFSRTSDR
                     VCFLDYVEEDSLPGLYAGASLLVLPSFEEGFGLSPLEAMACGTPVIVSNGGALPEVVG
                     DAGLIFDLSKPDTLAESMKICLSDSNLQQSLKERGLARAKLYSWQNTAELVWNTLNEI
                     "
     gene            complement(2890..>3929)
                     /locus_tag="VFQ23_09500"
     CDS             complement(2890..>3929)
                     /locus_tag="VFQ23_09500"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9906866.1"
                     /translation="LFATLSVLWSADWRTSLYIALHLWLLFLFILSLRDWREAWRPAM
                     LGFCAALSIQIIASFVEFAAQSTHFLLPLHSNWPGPFDPSVRGVSVVQFADGIRVLRA
                     YGTTPHPNILGGFLLLALLGPTSIFLANKKPNYLALVLLCLGIIALVLTFSRSAWLGF
                     VVVILLLFLKSGHFDRRHLFLLLAASALTFLLTVYSLRDLVFTRVSGSQVETEQLSRN
                     GRSWLNQQGLETLRQYPLTGVGIGAFIITLSKHALEGAPIEPVHNILLLAGTELGVFG
                     MLILIILFVSIAWKFIQSKTRNSILSGVILAGLGVIGLFDHFLWTLAPGRVMLALAFG
                     LWAGSIKDHAA"
BASE COUNT         1026 a          984 c         1025 g          894 t
ORIGIN      
        1 tgaccggagt ttgatccttc cagatatgct cggggagaat gaaggtgcct gcaagccctt
       61 cgttcaggaa gaacggctgc ggttccttga gcttgatcgt aagtccataa tccccattgg
      121 cttccatcgt atcgatggcg gccaggttgt tggcacgtcc tgcggcgatc aggaagttgt
      181 agctgaagat cacatcatca acggtgaagt cttcgccgtc gtgccatttg acaccttcgc
      241 gcagggtaac gtcccaggtg agtccatctt cactgggagt tgctgacgta gctagcgagg
      301 gaatcggctc aagctcgggg gtgcgaatgt aaagcgagtc gtagacccag cccaccagcc
      361 aaaagccgat gaaggtattg aaattggctg gggtaatcgg tccctcgtcg ttgtcgatca
      421 aaacggtaag tggacccgca ggtgcctctg tcggaacctc tgttggtgcc tcggttggca
      481 tttccgttgg gatgtcggtt acgaccgggg cttgcgtctc agccggtgca gccgtttccg
      541 ttgcagccgg cccgcatgcg gaaagcagca cactggcaag cagcagcgtg cttaaaacgt
      601 agtacagctt gtttctcata gaacttctcc tccgaaattc gtttgggaat aatcgtgctc
      661 tcaacgtggc aaataaaata tgggtcgttt acctccatat tcagaaaaat gtttgaattt
      721 gagggattgc ggcgtaaaac agtaatgatt ctatactata tacgatgatc cgtgcatttt
      781 gaatctgtgg aacactacga aaacagaatt tccgtcacat cgtgggttct acgaactcga
      841 caaagcggtc gataaaacgc gttttatcga atcgtttggc ttgctgcacc aggtcatcaa
      901 cgcagaagga actatgtatg cgctcgaatt gcaggatggc ttcgatcaaa ctttctttgg
      961 tttgttcaga aaatagaacg ccggtcgtgc catccagcac ggtttcgagt gcgccgccgc
     1021 gcccatagac aataacgggg cagcccgcag cctgtgcctc aacgatcgca atcccaaaat
     1081 cctcttctgc cgcacagacg aaacctcgcg ccttgctcat taattcaatc acactttcat
     1141 ccgtttgata acccaggaac ctgatgttcg agcctgccag agctcttaag cgtggcagct
     1201 ctgcaccatc tccgatgata agcagaggca ggttgagttc tgtgaaggcc tccaccagca
     1261 agtcgattcg cttgtgcggc acgagacgcg ataccgtgac atagtaatct tcacgctgcc
     1321 cgtttggttg aaagcgatcc acttccaccg gcgggtagat cacgggcgct tcacgctgat
     1381 aggcagactg aatccgcctg gaaacagcct gtgacacagc tgcaaactga tggacacgcg
     1441 cggcggcttt gcgatcccat ctgcagaacg cctgcaggag tttgtcaacg atgatatttc
     1501 tgttggtatg agttccatcg agattaatat cagtccaggc gtaacgcatg ggtgtgtagg
     1561 tataggacac gtgacgcgcg ccgttgaagt tatgcacgcc atgtgctacg gcatagctga
     1621 atgaaacgat caggtcgtac ttctgaagat taaatcgttc gatggcggcc ggcatcaggg
     1681 gcaggaacat acgatgatgt ctgtgggcca acgggaagcg atcgaggtac gatgttttga
     1741 tctctcgttc tgcgacaggc gtgctcttga atacttcctt gttatagatg agcgtaaaga
     1801 tatctgcttg tggatagatt tcaagcgcgg taaataggac cttttcggcg cccccaagcg
     1861 agggaagtga atcgatcaca aacgcgatgc gcggattaga tttcattcag tgtattccag
     1921 acgagctcag ctgtattttg ccaggaatac aactttgcgc gcgctaatcc tctttctttc
     1981 agtgactgtt gaagatttga gtcactcaag caaattttca tggattcagc taaggtatca
     2041 ggcttcgata aatcaaaaat tagtcctgca tcgccgacga cctccggcag tgcaccccca
     2101 tttgacacga tcaccggcgt tccacaggcc atggcttcca gcggagacag tccaaacccc
     2161 tcctcgaagg aaggcaggac gagcaggctt gcgccggcat ataaccccgg caggctgtcc
     2221 tcctcgacat agtcaagaaa acacactcgg tcgcttgtgc gggaaaaatt cacgggctga
     2281 aatacacgtc cgcttgcgcc tgcaataact agccaggtat cttgaaaatc gctctttact
     2341 tcaccccagg cttgcaaaag tgcaggcagg ttcttacgtg gttcgagcga accgacaaac
     2401 aagacatagc gtgccggtaa attgtacttt gtttgtttcg ctttgagcgt aaacctgcaa
     2461 tggtctacac cgtttggtgt gacttttaca tttctgatgc cgaaacgtga cgtcactttt
     2521 tgtcgtacat attccgaggg cgtaaaaacg attctgacac gtttgaccag gatcggcagg
     2581 aacatgcgat accacatggc aaaagtgctc tggaaccatt ccgggtgttc aatcggactg
     2641 agatcatgga tggtcaatgc ctggttggaa accatcaggg gacccgtatt tgccggagac
     2701 cataagatag agtttgcgcc taacttttgc ggcaacacga attgctccca cgtgtgacct
     2761 ctcaacccgg ttgaggctag cgatggtttt gctatgctgc agtcagtggg gaggtgtgaa
     2821 agcaactcac gcgcatagcg ctccacacca gtgactcgcc gtgccagaaa ccgcccattg
     2881 ataacgacat taggcggcat ggtcttttat gctcccagcc cagagaccaa aggcaagcgc
     2941 aagcatcact cgacccggtg ccagggtcca gagaaaatgg tcaaagagac cgatgactcc
     3001 cagccccgcg aggatgacac cagacagaat cgagttcctg gttttggatt gaataaactt
     3061 ccatgcaatc gaaacgaaga gaataatgag tatcagcatg ccaaagacgc ccagctctgt
     3121 gccggccagc aataggatat tatgaaccgg ttcgatgggc gcgccctcca gcgcatgctt
     3181 cgatagcgta ataataaatg cgccaatccc aacgccggtt aatggatact gacgcagggt
     3241 ttccagtccc tgttgattta gccatgagcg accattcctc gaaagctgtt cggtctccac
     3301 ttgggagccg ctgacgcgtg tgaaaaccag atcgcgcaat gaatatacag tcagaagaaa
     3361 agttaacgcg ctggctgcca ataataagaa taaatgcctg cggtcgaaat gacctgattt
     3421 cagaaacagc aataagatga caacgacgaa ccccagccag gcggaacggg aaaaagttaa
     3481 aacaagtgca atgatcccca ggcataacaa gaccaaagca agataattcg gttttttgtt
     3541 cgcaaggaat atggaggtag gtccaagcaa agcaagcaat agaaatccac ccaaaatatt
     3601 cgggtgaggg gtggtgccat aggcgcgtaa gactcttatt ccatccgcga attgtacaac
     3661 gctgaccccg cgcacagatg ggtcaaacgg acccggccaa ttggaatgaa gcggaagtag
     3721 aaagtgagtc gattgggcag caaactccac gaagctcgca atgatctgaa tacttaatgc
     3781 agcgcaaaat ccaagcatcg ccggtctcca cgcctcgcgc cagtcgcgga gagacagaat
     3841 gaataagaac aatagccaaa gatgcagggc gatatagagt gatgttcgcc agtctgccga
     3901 ccagagaaca cttagagtgg cgaaaagaa
//
LOCUS       DASTJP010000319         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_887649, whole genome shotgun sequence.
ACCESSION   DASTJP010000319 DASTJP010000000
VERSION     DASTJP010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887649"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFQ23_09505"
     CDS             complement(<1..797)
                     /locus_tag="VFQ23_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9906867.1"
                     /translation="MKRVGLLLIFLGSLLSLFQPARAQSDEPLAIVMTADGPIVPPML
                     EYIKRGIERADRQNAEVLIIQLNTPGGSVETMLEIIREIRASRVPVVVYVAPRNAIAG
                     SAGAMITMAGHASAMAPETSIGASSPISSSGENLNSTAEAKAKEITKAAIRDYVRPRG
                     DAALALAESMIDEAKAVTATEALEVNLIDFIADDVEDLLQALNEFSVQLNNETRTLNT
                     SNIRTESFDMSFIEEFLLLLVDPNIAFLLIAIGVQAVLIEISSPGGWV"
     gene            1157..2536
                     /locus_tag="VFQ23_09510"
     CDS             1157..2536
                     /locus_tag="VFQ23_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906868.1"
                     /translation="MNSKRFIPDALWIIMPALIGMVFILYVFYPGYMSNDSVDQLTQG
                     RSNNYTDWHPPLMSWVWGRFDQVLPGPAGMLLFHNLLFWGGLGLFMQSVAPKWPGWLK
                     SILILLVGFFPPILMLLGVIWKDVGMAAAFLFGTALLVQAQERKSAFFLSAGLVSIWY
                     GFSIRLNGIIAAVPLIFFAAAIFYDLGYFAKWKHANKTLQIFLSGVVLLGVMLFATKA
                     VEKVLVKNPPSYPIQQIFIHDLVGISVRLSEVLLPDYLLLPVPPTQMELRRIYTPGTI
                     NHLFFGDDGRIRLKLTNDPVLISNLRSTWAKVVLDHPKAYLIHRSRIFYRQYWLEGRR
                     VCFPYQEVTTPNALGVKSPQSPLTASLFAWAEPVTTTFLYRGWIYLLITFLLVLYNFL
                     YALFNRMKLPLPAVILALSTSGLLFGAAYFFISPACDFRFHYWVVISAILSMIATVYF
                     KTHPGNDQQ"
     gene            complement(2889..>3438)
                     /locus_tag="VFQ23_09515"
     CDS             complement(2889..>3438)
                     /locus_tag="VFQ23_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9906869.1"
                     /translation="FPGRNLINALVDMPFAISPVVAGYMLLLLFGRNGLLAPLVEATG
                     IRVAFAFPGMLLATLFVTFPFMIRELIPVLEAFDRRQEKAAATLGANGWQIFWRVTFP
                     ALRWGMIYGVTLTFARALGEFGAVLVIGGAAQGSLMTTTLFIYHALEERQYIAAYSAA
                     LALGLFSLILVLGSDWLRRRKH"
BASE COUNT          786 a          904 c          849 g          899 t
ORIGIN      
        1 acccatccac ccgggctcga aatctcaatc agcaccgcct gtaccccgat cgcaatcagc
       61 aggaaggcga tgttgggatc gaccagcagg agcaggaatt cttcgatgaa actcatatcg
      121 aacgattcgg tgcggatatt cgaggtgttc agcgtgcgag tctcgttgtt gagttgaacg
      181 ctgaactcgt tcagtgcttg cagtaaatct tcgacatcat cggcgatgaa atcgatcagg
      241 ttgacttcca gcgcttcggt cgcagtgacc gcttttgcct cgtcgatcat cgactcagcc
      301 agggccagcg cggcatcccc gcgtgggcgc acgtaatctc gtatggcggc tttggtgatc
      361 tcttttgctt tggcttccgc agtggaattg aggttttcgc ctgaactgct gatggggctc
      421 gacgcgccga tagaagtctc aggagccata gccgaagcat gaccggccat ggtgatcatc
      481 gcacctgcgc tgcccgcaat ggcattcctg ggtgccacgt acacaacgac gggcacacgg
      541 ctggcgcgga tctcccggat gatttccagc atggtttcca cacttccacc cggtgtgtta
      601 agctgaatga taaggacttc ggcgttctgt cgatctgccc tttcaatgcc gcgcttgata
      661 tattccagca tcggcggcac gatcggccca tctgccgtca tgacgatcgc cagcggctcg
      721 tcactttggg cgcgtgccgg ctggaagagg cttagcaatg aacctagaaa aatgagcagg
      781 agcccgacgc gtttcatgga gtgattataa cccgtccctt gcatcctcct gacggacaaa
      841 gggtggaggg gtgtccaatt tagaggatac actctgctat gtcaccctga gcagagcgaa
      901 gggtctctcc agaggttggg tgaattcgct caacctctgg gattcttcgt tgggtgccgc
      961 tcactcagaa tgacatgatg tttctgctgt tcgacactcc cagggacgga ttgatcatct
     1021 gattagctca tacccgaacg gaatgtaccc agacgtattt acgcaggttg gcaaaaagtt
     1081 gacgactggc atcaatcgta ggttgaattt agaatataag aaggttttct tattgcttct
     1141 catcaagaaa tcttcaatga atagcaaacg ctttataccg gatgccttat ggatcattat
     1201 gcctgctctt atcggcatgg ttttcattct gtacgttttt tatcccggct atatgtcaaa
     1261 cgactctgtg gatcaattaa cccagggccg ttcaaataat tacaccgatt ggcaccctcc
     1321 gctcatgagc tgggtatggg gacggttcga tcaagtcctc cccggcccgg ccgggatgct
     1381 cctgtttcat aaccttctgt tttggggagg gctcgggctg tttatgcagt ctgttgctcc
     1441 aaaatggcca gggtggctta agtcaatttt gatcctgctt gttggctttt ttccaccgat
     1501 actcatgtta ttgggcgtca tctggaaaga cgtcggaatg gcggctgcct ttttgtttgg
     1561 cacagcactg ctggttcagg cgcaggaacg caagtctgcg tttttcctgt cagccgggct
     1621 ggtttctatt tggtatggct tctcgatcag acttaacggg atcatcgctg cagtgccttt
     1681 gatatttttt gcggctgcta ttttttacga tttgggctat ttcgccaaat ggaaacacgc
     1741 aaataagact ctgcaaattt ttctttctgg cgtcgtttta ctcggtgtca tgctcttcgc
     1801 aacaaaagct gtggaaaaag tgctcgtgaa gaatccaccc agctatccaa ttcaacagat
     1861 tttcatccat gatttggtgg gaatttcagt ccgattaagc gaagtgctat tgccggacta
     1921 tctgcttctc cctgtgccgc ccactcaaat ggaactgcgc agaatttata caccaggaac
     1981 gataaatcat ctcttctttg gggatgacgg acggataagg ctcaaactca cgaatgatcc
     2041 ggtgttgatc tcgaacctgc gctcaacctg ggcaaaggtt gttcttgatc acccaaaagc
     2101 ctatctgata caccggtcga ggatctttta tcgccaatac tggcttgaag gaaggcgagt
     2161 ttgttttccc tatcaggaag tcactactcc taatgccctt ggagttaaat ccccacagtc
     2221 accattgacg gcgagcctgt tcgcatgggc agagcctgtg acgaccactt ttctttatcg
     2281 cgggtggatt tacttactta ttacgtttct tctggttctt tacaattttc tttatgccct
     2341 gttcaaccgt atgaagcttc cattgcctgc tgttatattg gctcttagca ccagcggctt
     2401 gttgtttggc gcagcttact tttttatctc accggcctgc gatttccgct tccattattg
     2461 ggtggtaatc tcggcaattc tctcgatgat tgcgaccgta tatttcaaga ctcatcctgg
     2521 caacgatcaa caatgagcga actaaccttt accctccata ccctacgcgt cataacgggt
     2581 ctggacctac ctgcacgctt gcacctaggc tcatgcagac tgaacgatga tcaaggttga
     2641 aatagaagtc cggtccgttt tgttgagcta accttcaaaa gaaatgggcg ggacgctatg
     2701 cgcgtcccgc ccgatgattc tataaccata actctatgat gctttatttc gaattcctgc
     2761 ctttatcttt ccgctgtgca tcgtagagag ttccgacaaa gctaagaaag ataaacagag
     2821 ccagtaccgc gtagattcca aaacaggttt gtagcatgtt gaacctccgg ttctcagcct
     2881 cacaactact aatgcttcct gcgtctcaac caatctgaac cgagcaccag tatcaaagaa
     2941 aataaaccta acgccagcgc ggcactatag gccgcaatat actgacgttc ttcgagcgca
     3001 tgataaataa acaaggtggt tgtcatcagg ctgccttgcg cggcaccgcc gatcaccagc
     3061 actgcaccga attcaccgag tgcgcgtgca aacgtcagag tgacgccata gatcatgccc
     3121 cagcgtaggg cagggaaggt cacgcgccag aagatttgcc agccattcgc cccaagagtc
     3181 gcggcggctt tttcctgccg ccgatcaaag gcctccagca ccgggatcaa ttcgcggatc
     3241 ataaatggga aggtgacaaa gagcgttgcc agcagcattc ctgggaatgc aaacgcaacc
     3301 cgtatgcccg tggcctccac cagtggagcc agcaacccgt tgcgcccgaa gagcaacagc
     3361 agcatatagc ccgccaccac cggcgaaatg gcaaatggca tatccaccag ggcgttgatc
     3421 agattacggc ccgggaag
//
LOCUS       DASTJP010000320         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1057876, whole genome shotgun sequence.
ACCESSION   DASTJP010000320 DASTJP010000000
VERSION     DASTJP010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057876"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..437
                     /locus_tag="VFQ23_09520"
     CDS             45..437
                     /locus_tag="VFQ23_09520"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9906870.1"
                     /translation="MEMIIDFPGGSRVDAHFGGFTVPTDQPPAGSAPTPFATFLSSIG
                     TCAGIYVLGFCQQRGIPTDGIRIVQRVNSNPLTHMVDKIDLEIQVPSSFPDKYRDSLI
                     RSAELCAVKKHLEKPPLFDITTTKVEIA"
     gene            455..1123
                     /locus_tag="VFQ23_09525"
     CDS             455..1123
                     /locus_tag="VFQ23_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9906871.1"
                     /translation="MEKFLNEQIVKQIEEAFAAMENPVQVLYFGSQDHCDSCTETQQL
                     LEEVTATSDKIELSVYDLKDNREIAERFNVTNAPGIVIAAKNDTEVKNLGVQFSGAPS
                     GYEFSTLINDILAVSRRDSGLSETTRELLKKLDRPVHLQVFVTPSCPYCPRAVLLAHQ
                     MAMENPQMIRAEGVEATEFPELADRFNVRGVPQTVINSGAGIVVGAVPEQNLLAEIMR
                     ALQN"
     gene            1151..1726
                     /locus_tag="VFQ23_09530"
     CDS             1151..1726
                     /locus_tag="VFQ23_09530"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9906872.1"
                     /translation="MKKVRRRSQKPYPILPMAAGLLLIGFALVIMTSPKSGSAAESSS
                     SVVPVAVSFAAPELSLENLNGQTESLADYRDHVVLVNNWATWCPPCKAEMPTLAAYYR
                     EHQSEGFTIIAVEAGDPVETVAPFVQNYNLEFPVWLDPGGVSVRTFGNGTLPNSYVID
                     RAGTVRYAWTGEINKAMLEKYVTPLLMEDGR"
     gene            1761..2012
                     /locus_tag="VFQ23_09535"
     CDS             1761..2012
                     /locus_tag="VFQ23_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HET9906873.1"
                     /translation="MTETKPQPRVIVFSTPTCSFCNMAKKYFREKGVKFKDVDVSRDP
                     IAARDMIRRSGQQGVPVIDIGGRIVVGFDRVKIDKYLSL"
     gene            2037..2306
                     /locus_tag="VFQ23_09540"
     CDS             2037..2306
                     /locus_tag="VFQ23_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET9906874.1"
                     /translation="MNLQPMGTRVLIKPLEQESKTSSGLLLPETAKEKPQTGLVVAIG
                     DDEEIKLKVNDKILFAKYSGTEFKMDGTEYLLLEANDVLAKFLVN"
     gene            2484..3932
                     /locus_tag="VFQ23_09545"
     CDS             2484..3932
                     /locus_tag="VFQ23_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HET9906875.1"
                     /translation="MNISKHLTIMQMNDSHAYLDLHQELFWKGNHAEYRKAGGYARIA
                     AILNRVREEKSGQVLAFDGGDTIHGTYAAVKTQGETLIPVLNRLGFDAMTAHWEFAYG
                     PEQFKKVAGQLAYPVLACNVYDQKSRNLIFPAYTIHEVGSLRVGTIGIASNIVDKTMP
                     PSFSQGIYFTLGNEELPGIITKLRNDENVDLVIVLSHLGFPQDVKLAQEIQGIDVILS
                     SHTHNRVFAAKQVGHTIIMQSGAHGSFLGQLDLEIDNKRIIDFGHQLHIVEERVPPDQ
                     TVQTLVDQALAPYRGELHKVVGHTSTALHRNTMLESTMDNLLLQSLLDHTGVQIAFSN
                     GWRYGAPIAPGPVTLNDLWNIIPVNPPISLVELTGEEIRAMLEENLEHVFARDPYHQM
                     GGYVKRMLGLNLYVKIENDVGQRIQQLFVQGEPIQMDKLYTATFVTAQGVPQKYGSGR
                     RDLDIHAVEALQQYVSQNTPVDADLRGTVMAV"
BASE COUNT         1050 a         1009 c         1054 g          958 t
ORIGIN      
        1 gataccaaaa aaattacatt gtgtaacaag gagtattacc tcacatggaa atgatcattg
       61 acttccccgg tggctcacgt gtggatgccc attttggtgg ctttactgtc ccgaccgacc
      121 agcctcctgc tggcagtgca ccgacgccat ttgcaacctt tctctcttcc attggcacgt
      181 gtgctggaat ttatgtgctt ggtttctgcc agcagcgtgg cattcctacc gatggcatcc
      241 gcatcgttca gcgtgttaat agcaatccgc tcacccatat ggtggacaag atcgacctcg
      301 aaattcaggt gccgtcttcc tttcccgaca agtatcggga ctcgctcata cgctcggcgg
      361 agttgtgtgc ggtgaagaag cacctcgaga aaccgcccct attcgatatt acaacgacca
      421 aagtcgaaat tgcctgatgg cgcgggagaa gaccatggaa aaatttctga acgaacagat
      481 cgtgaagcag atcgaagaag cctttgcagc gatggagaac ccggtacagg tgctttattt
      541 tggctcacag gatcactgtg actcttgcac cgaaacgcag cagcttctcg aagaggtcac
      601 cgcgaccagc gacaagattg agttgagcgt ctacgatctt aaggacaacc gcgagattgc
      661 ggaaagattt aacgttacga atgcacccgg catcgtcatc gctgcaaaaa acgataccga
      721 ggtaaagaat ctcggggttc agttctcagg ggccccttca ggctatgaat tcagcacgct
      781 catcaacgat attctggcgg tctccagacg agactctgga ctcagcgaaa cgacccgtga
      841 gttgctgaag aagcttgacc gacccgtgca cctgcaggtg ttcgtcaccc caagctgtcc
      901 atactgtccg cgtgctgtgc tgcttgccca tcaaatggcg atggaaaacc cgcagatgat
      961 ccgtgccgag ggcgtggaag caactgaatt ccccgaactg gcagatcgct tcaacgtgcg
     1021 cggcgtgccc caaaccgtga tcaattccgg tgcagggatt gttgtcggcg cggtacctga
     1081 gcagaacctg cttgccgaga tcatgcgcgc acttcagaat taatctcaat cgcgagtcag
     1141 tgtcatggca atgaagaaag ttcgccgcag atcgcagaaa ccgtatccta ttcttcccat
     1201 ggcggcgggt ctgttattga tcggttttgc cctggttatt atgacttccc caaagagcgg
     1261 gagcgccgca gaatcttcat cttctgttgt gcctgttgct gttagttttg ccgctcccga
     1321 actctcgctc gaaaacctga acgggcagac agagtcgctg gcagattacc gcgatcacgt
     1381 agtacttgtc aacaactggg cgacctggtg cccgccctgc aaggcagaga tgcccacttt
     1441 ggcagcgtat tacagggagc atcagtccga agggtttacc atcattgccg tcgaagctgg
     1501 cgaccctgta gagaccgttg cgccgttcgt ccaaaattac aacctggaat ttccggtctg
     1561 gctggacccc ggcggagtat ctgtgagaac ctttggcaat ggcacactgc ctaactcgta
     1621 tgtgatcgac cgcgcaggga cagtccgcta cgcgtggacc ggcgagatca acaaagccat
     1681 gcttgagaaa tacgttaccc cgctgctgat ggaagacggt cggtaaaaca gatcctaaac
     1741 attttcagta gagaactatc atgacagaaa ccaaacctca accgcgtgtg atcgtgttca
     1801 gtacgccgac ctgttcgttc tgcaatatgg caaaaaaata ctttcgtgag aaaggcgtca
     1861 aatttaagga tgtggatgtc agccgcgatc cgattgccgc ccgggatatg atccgccgct
     1921 ccgggcagca aggagttccg gtgatcgaca tcggtggaag aatcgtggtc ggcttcgacc
     1981 gcgtcaagat cgataaatat ctgagtctct aattttcgga ggtagaaatg acaaaaatca
     2041 atcttcaacc catgggcaca cgtgtcctca ttaaaccact tgaacaggaa agcaagacct
     2101 catccggtct gttgctgccc gaaacagcca aggaaaagcc tcagaccggg ctggtcgttg
     2161 ccattggcga tgacgaggag atcaagctga aagtaaacga taaaatcctg ttcgccaaat
     2221 atagtggtac cgaattcaag atggatggga ccgaatattt gctgctggaa gcgaacgacg
     2281 tgttagctaa gtttcttgtc aactagctcc agattgcatc actttaaact gcaacgcgca
     2341 agtatgatag actaaaactc acaaaattaa ggtgccattt ttttgatgta aataccaaac
     2401 atgccctata aaagcttaat tcatcactgc ccagatcatt gggcagtatt gttttattgg
     2461 cacatgaatg aggagatcaa aacatgaaca tcagcaaaca tttgacaatc atgcagatga
     2521 acgatagcca tgcctacttg gacttgcatc aggaattgtt ctggaaaggc aatcatgcgg
     2581 agtatcgcaa agcaggcggc tatgctcgga tcgcggccat actcaaccgg gtccgtgagg
     2641 aaaaatcagg gcaagtgctc gcgtttgatg gtggcgatac gattcacgga acgtatgccg
     2701 cggtcaagac gcaaggggaa accttaattc ctgttcttaa cagattgggt ttcgacgcga
     2761 tgacggctca ctgggagttc gcctatggtc cagagcaatt caagaaagtg gccggacaac
     2821 ttgcttaccc ggtgttggcc tgcaatgtat atgaccagaa atctcgtaac ttaatctttc
     2881 cagcatatac gatacatgag gttgggagcc tgcgcgttgg cacaattgga attgcctcca
     2941 acattgtaga taaaaccatg ccgccgtcct ttagtcaggg aatctatttc acgctaggga
     3001 atgaggaatt gccagggatc atcacgaagt tgcgtaatga tgaaaacgtg gatctcgtta
     3061 ttgtcctatc gcacctgggc tttccacagg acgtgaaact agctcaagaa atccagggca
     3121 tcgatgtcat cctcagtagt cacactcaca accgcgtatt cgctgcgaaa caggtcggcc
     3181 atacgatcat catgcagtct ggagcacatg gctcgtttct aggacagctt gatctggaga
     3241 tcgataacaa gcgaatcatc gatttcggac accaacttca tattgtagaa gaaagggttc
     3301 cgccggacca gaccgttcag actctagtgg atcaggcgct agccccatat cggggagaat
     3361 tgcataaggt ggtgggtcat acctctacag ctcttcatcg caacacgatg cttgagtcga
     3421 ccatggataa ccttttgttg cagtcccttc tggatcatac gggagtgcag atcgcattct
     3481 cgaacggctg gcgctatggc gctccgattg cgccaggccc ggtgactctg aatgatctct
     3541 ggaatatcat tcccgttaac ccgccgattt ccctggtcga attgacaggc gaggagattc
     3601 gagcaatgct ggaggaaaac ctggaacatg tctttgcgcg tgatccgtat catcagatgg
     3661 gtgggtacgt gaagcggatg ctggggctga atctatatgt gaagatcgag aacgatgtcg
     3721 gacaacgcat tcaacaactg tttgtgcaag gtgaacctat acagatggac aaactataca
     3781 cagcaacatt tgtcacagcg cagggtgtgc cccaaaagta tgggtctggt cgcagggacc
     3841 tggatattca cgccgttgag gctttgcagc agtacgtgtc ccagaatact cctgtcgatg
     3901 cggacctgcg aggaacggta atggctgttt gagtgaacat gtgaattgtg agataaacaa
     3961 aaggcatcat gtttgtggaa ccatgatgcc tttcccgggt cgttgcaatc gactacttac
     4021 cggctcgaat ttttgggccg ctcatgggat tgccaaatag tggggccaat a
//
LOCUS       DASTJP010000321        19327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_955021, whole genome shotgun sequence.
ACCESSION   DASTJP010000321 DASTJP010000000
VERSION     DASTJP010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19327
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955021"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFQ23_09550"
     CDS             <1..266
                     /locus_tag="VFQ23_09550"
                     /inference="COORDINATES: protein motif:HMM:NF020694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HET9906876.1"
                     /translation="KGVAAVIIECLEAAEALGMTEYARAQMLKIISDEPMIDRFVSGS
                     IKHAKRRVEEMEAVVEMLNSISVSSFTSQASIKRLKEIMETRE"
     gene            263..976
                     /locus_tag="VFQ23_09555"
     CDS             263..976
                     /locus_tag="VFQ23_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET9906877.1"
                     /translation="MTKKILVIGAHSADFVWRAAGTIAIITSQGGSAKVIALSYGERG
                     ESGELWKEPNQTVENVKRIRHEEAQAAAEAVGAEFQCLDLGDYPLNMTDGATEQLIDI
                     FQDFAPNIVLTHNPVDPFNPDHPVASHMVQKARLLSTGAGVASAFSRITPPDLYFFEP
                     HQPELCDFKPNVFVDITSVMDMKSKAMDAMKAQGYLHKYYSELATRRANHARRISGNS
                     EIKFAEAHFRTLPWVVSGL"
     gene            973..1605
                     /locus_tag="VFQ23_09560"
     CDS             973..1605
                     /locus_tag="VFQ23_09560"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906878.1"
                     /translation="MNPHDLHRLSELGVATVYEASGREGLIDIPLIQLIPNSRAAGPA
                     RTAACGQDDNLMVHAVIEQIQPGEVLVLAMPEPAPVALIGELLATQVKVKQAAAILVD
                     ASVRDVSELVELGLPIWTHFIRVRGATKTKIGELNTTVTVGGTQISNGDIVVLDVDGA
                     VCVKRERVYEVLRASEERLVKEASLRKKLSAGETSYDLHGLRVYVEGNKK"
     gene            1602..2561
                     /locus_tag="VFQ23_09565"
     CDS             1602..2561
                     /locus_tag="VFQ23_09565"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HET9906879.1"
                     /translation="MTTQAEPIFDIAHLAHVELLTPKFNESLRFFVDVMGMTESGREG
                     DSVYLRGWDDYEHHSLQLTAAAKPGLGHYALRATSPQALQRRVEAIEQTGLGKGWKEN
                     NIGHGPAYCFTDPDGHQIEIYYETEWYQAPAELKPALKNQASRYSDRGVGVRRLDHLA
                     LLAADVRSNRLFMTQTLGGRVTEQIVFDDGSEKGAWLTFNNKSYDVTFAEDSYGAKGR
                     LHHFTYALDSREAILRAADICLENGVFIETGPHKHAIQQTFFLYVYEPGGNRFEIANA
                     GARLILAPDWKPIVWNEEERKKGQAWGLKTIESFHKHGTPPVE"
     gene            2574..2954
                     /locus_tag="VFQ23_09570"
     CDS             2574..2954
                     /locus_tag="VFQ23_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906880.1"
                     /translation="MPHLTLEYSDNIEVDVPLLLTRLHEEVVATGAINLKGIKSRAIE
                     HTQYLIADGDPEYAFVHVALLIREGRPIEVQKDATQRVMKVLKQTFGHLFETRKLSLT
                     VDLKEMRDGIALTDHNIPDKSKSG"
     gene            3022..4206
                     /gene="pobA"
                     /locus_tag="VFQ23_09575"
     CDS             3022..4206
                     /gene="pobA"
                     /locus_tag="VFQ23_09575"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: protein motif:HMM:NF006091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HET9906881.1"
                     /translation="MRTQVGIIGSGPSGLLLARLLHLQGIDTVILESRSREYVQGRIR
                     AGVIEQGMADLLREAKAGERMDREGLIHEGINIAFDNRLERIHFPSHTNGKVVMVYGQ
                     TEITHDLMKAHIDEAGGIVHYESPAIRVEDYNSDSPRIIYQQNGEQKELTCDFVAGCD
                     GFHGVARQSVSADKIKVFERTYPVGWLGILADVPPVRDELVYVNHERGFALCSMRSHT
                     RSRYYIQCRQSDKVENWTDDAFWDELRRRLPPHVAEDVTTGPRLEMSVTPLHSFVAEP
                     MRFGRLFLAGDAGHIVPPTGAKGLNLAASDIYYLSRALLAYYKDKRTDLLDRYSEIAL
                     RRVWKAIRFSWWYTTLLHKFDEDPMTYRLQIAELDYLTGSAAGLTTLAENYVGLPYEI
                     FE"
     gene            4203..5369
                     /gene="pcaD"
                     /locus_tag="VFQ23_09580"
     CDS             4203..5369
                     /gene="pcaD"
                     /locus_tag="VFQ23_09580"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HET9906882.1"
                     /translation="MNFATLNGTNLHYRSDGDTQGTPLVLIHSLGTDLRLWDDLIPHL
                     DPALRVVRLDLRGHGLSADPAGELALVDFSTDIAALLDHLEIDKAIILGISIGGLIAL
                     DFALRNQERVSSLILADTAARIGTTEFWQTRMDTLRAHGMEHLADSILARWFAPDFAT
                     RCPVQFQGLRTLFNRNSVAGYNAACAVLRDADLRTEVSQITVPALVLCGAQDAATPPD
                     VVQELAESLPSAHFAVLPGSGHTPPIEQPQIMADQINAFLRAHRTVGDIYEQGMKIRR
                     EVLGDAHVDQAEVNKTSFDADFQRFITETAWGSVWARPGLDRRTRHLLTLAMLAALGK
                     EHEFAMHVRASRNTGVTMDEIKEVLLQVAVYAGVPAANSAIAAAKRVIAEFEEK"
     gene            5372..6079
                     /gene="pcaH"
                     /locus_tag="VFQ23_09585"
     CDS             5372..6079
                     /gene="pcaH"
                     /locus_tag="VFQ23_09585"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET9906883.1"
                     /translation="MANSQPFKPLDKERHPPYLYPGYKSTVPRSPTRPLIPLRQSLAE
                     LTGPVYGLDAIHPLDNDLTRNAVKDADPQGQRIIVTGRVLDDTGRPIPNALVEVWQAN
                     AAGRYLHHNDQHDAPLDPNFHGGGRVLTDGEGMYTFTTIRPGAYPWGNHFNAWRPAHI
                     HFSIIGVNFQQRLVTQMYFPGDPLIPLDPICNSIPDENARQSLVATYDHEITQPDWAL
                     GYRFDMVVCGPKMTPFE"
     gene            6081..6647
                     /gene="pcaG"
                     /locus_tag="VFQ23_09590"
     CDS             6081..6647
                     /gene="pcaG"
                     /locus_tag="VFQ23_09590"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HET9906884.1"
                     /translation="MNPLHQTPSQTVGPFLRIGLVHGEKQNVLADEHMAGKHIRLAGV
                     VYDGNGKPIEDALVEIWHADANGIYPHPVDPRHSEADSHFRGFGRAENEDGGLYEFHT
                     IKPGGRDGHAPFINLHLFARGMLLHAQTRIYFADEQANATDPVLQSVPVERRATLLAR
                     LEENGEIPVYRFDIHMQGDQETVFFELE"
     gene            6650..7984
                     /gene="pcaB"
                     /locus_tag="VFQ23_09595"
     CDS             6650..7984
                     /gene="pcaB"
                     /locus_tag="VFQ23_09595"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HET9906885.1"
                     /translation="MPASPSDSRIFSPLFNSPAMAEIFSDEQFVRCLLKVEGSLARVQ
                     GQLGVIPAGAAEQIASKVGTLSVDFDRLRSGTEQAGFPVIELVRQLKAHVGNDFADYI
                     HWGTTTQDVMDTALVLQIRAAIAILESGLKTLIQSLARLADQHRHTLMAGRTHSQQAL
                     PIPFGLKVAGWLAPLIRHCERLSQLKPRVLILQFGGAAGTLAALGKDGIPVRNSLSVE
                     LELGIPSMPWHTQRDSLAELANWLSLLTGSLAKMAQDVILLSQSEIAEVRESSDVARG
                     GSSAMPQKSNPVVSEFILVAHRTNASLLSSLHQALPQEHERGTYGQMEWLALPQMFAL
                     TGSAMEKAAWLGENLIVDADRMRANVAASQGLMLAEAVSYALAPHIGFAAAKKMVSDS
                     IRQAVTDGRHLLDIIREQSQAMLDWESLQDEGNYLGASETFIDAVLKSASQI"
     gene            complement(8206..10062)
                     /locus_tag="VFQ23_09600"
     CDS             complement(8206..10062)
                     /locus_tag="VFQ23_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906886.1"
                     /translation="MGFIMDGLDAETYDREYTDRQLLARIIGYFKPHFPMMGTIAIMI
                     TLNSVMDAAFPFLIARSLDQLEGASDLNVTIWQRTAWLIAAILVSGVLSWSFNFFRQK
                     YTARTVGNVVLKLRQDAFDAVLARDMSFYDEYSSGKIVSRVTSDTQDFSNVVTLTLNL
                     MSQVLIVIIVVSLLIFINLRLALITLAIAPIVVFIALAFRRIARYTTTQAQRVLAEVN
                     ANVQEAITGISVAKNFRQEQRIYDEFKPVNRQTYRLRLRQGLTFSSIFPILGTVAGIG
                     TALIVYFGGLSVITSSISAGSLYLFIESINLFWFPLISIASFWSQFQLGLAASERVFA
                     LIDAEPRVVQLDHKIAQRLQGRIEFRDLDFRYTENENVLKDFNLTIQAGETLALVGHT
                     GAGKSSLGKLVARFYEFQGGELLIDGQDIRSFNLDSYRGQLGIVPQTPFLFAGTVAEN
                     IRYGKPDATDEEVVTVASRVGGGDWLAALPQGLETPVGEEGKGISMGQRQLVALARVL
                     LQNPAVLILDEATASVDPLTEAQIQEGLDVVLQNRTAIVIAHRLSTIKSADRIIVLRK
                     GQIFEEGNHTSLMERGGHYAELYDTYFRHQSLEYIESWRKDQLEEQISPGNK"
     gene            complement(10080..11876)
                     /locus_tag="VFQ23_09605"
     CDS             complement(10080..11876)
                     /locus_tag="VFQ23_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906887.1"
                     /translation="MQNQAAVPSQSKREFSIADEHVYNRSSPVRWIISHLIRYPNFLA
                     SFLLAAALTNILFSAVPRLIGLAFDEALLPDPSSARLFTIAVTILGLVIVRGLLDITN
                     SWSVETLGQRLERDAREELYLSLLGKSQTFHNRQRVGDIMSRATSDVRQLNPMMNPGV
                     SLIIESSIGIISPLVFIAFLRAELLAAPLLFVIAHIFALRRYVNQLNPVAGEQRWRFG
                     EMNAGLTETITGIEVVKSAVQEEQERGKFLGNARRVRDLFVKQGEIEARYLPLLLLGI
                     AISGAFAHGVWLVSRNALSIGELVAYMGLMGVLRFPAFISIFTFSLVQMGIAGAQRIL
                     DLMKEETEVDENKAGHIGVLKGEIIVDHVTFRLDNDPDAAPVLRDISFRAAPGETIAI
                     VGQTGSGKTTLTKLVNRTYDVDEGRIVIDGIDVRDWNLDSLRSQISTIEQDIFLFSRP
                     IDENIAFSLGQQTDHAAIESAAKAAQAHDFIQTFTKGYKTIVGERGVTLSGGQRQRIA
                     IARALLTDPRILILDDSTSAIDSATEDQIQRAIRRVLEGRTTLLITHRLSQIRWADRI
                     LVLDRNQIVDQGTHEELMQRCDLYRRIFARYS"
     gene            12859..15972
                     /locus_tag="VFQ23_09610"
     CDS             12859..15972
                     /locus_tag="VFQ23_09610"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9906888.1"
                     /translation="MSENMVCLHIQLMGSAAISGGEIPLVLNQLKSRGLLYYLAATGE
                     SHSRDHLATLLWGDSSQSEAYHSLRSSLYHLRKSLRSVNAEEALISDGGSIGLSLAWY
                     ECDATEFDRLLKQGSEASLSQAVALYKGPFLQGFSLSDAHMFEDWVQMENTRLTHACS
                     EALSRLTTFAEEREAWEIAIGYVNRMIRIDPLSEAAQQRLMRLYLGQGEVGLALRQYR
                     QFENRLKQELGIAPNFETRVLYEDALRQQGGITIIPASHHTSKLTPNVLPFVGRDDLI
                     HELSSISADIEAGRGATVLIQGEGGIGKSRLIHEFASRLITKSQPWMILQGSCSPFDD
                     LLSHGPFIEALQNGSLDDLNDLLAESNASVPDARGRFFWRVLQTIRSMSNNSPLLLFI
                     DDLQWANSSTLNLFGFLSMRLHHLPVMLVGTVQHAEAIPALQRLITLGRRRHELRLLS
                     LTPLTQVAIADLLRASNVNPGSIETLAEWLNAKSAGNPFLLSEILAQLRTEIILKATS
                     DGWQLDTTQWLRWRTTFSLPETAHDLVGWRLANISQEARSLLDVLAVASQPMPESVLR
                     NIPGVWSNLFPALVDDLSARGLIMELTGNSFLMLPHHLLRETLLHRLSNLRRRTLHRQ
                     LAEAMEAKAYSDPNVWLRQIAMHAVAGEDVNRAHHYGMLLLPDLPYEYTGAETIDFVQ
                     HLHDLLAPTASPNEMILITRALGLLHQSLGHLETAGQWHRQTLEWAQKTNDYAAQAEA
                     YFEMSELALMSIDYRTARQTAEKGLEVIRATRGAVLLVPNGKGHRLLGASLAMEGRDL
                     AGAEEHLQKAVEIHRQIENQGDLCAVLFELGNVAAQRGELQRALDFYSESAHVAEAGR
                     IHYYLALARNNFAYHSLLLGRVDEAEQSVSQGIKMAEAYDLLAALLHLYSTKGEIQLH
                     LNKWNEAEVSFHSGLALAEELGSLERQAGYRGGLALAARGKKDFDMAQHLLEEALALI
                     AEQGYWHLRTRLQLWLAETLFEQADYDEATKLLDEAMQIARSQRRSLLVEQGEQLHAR
                     LLAIREK"
     gene            16395..17222
                     /locus_tag="VFQ23_09615"
     CDS             16395..17222
                     /locus_tag="VFQ23_09615"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HET9906889.1"
                     /translation="MPPVNSMDYSGKDTVLDVVRTDASEFFNIVDDPQNWNVQTRCTE
                     WEVRDMVGHMLDVTEGYLSRWESARNGESRNTAGLLVMADSLNKGAQSFRNLPRTEAI
                     QRLKDDYAKMMEIFQNLTADEWNNFIVTHPYMGGLPTFFYPAFHVMDYGVHTWDMHWG
                     LGDKDRKLDERTAGVLIPYMLYALLPSTVDQPSAKGVDVEYGIEVSGEWGGKWRVTVK
                     DGKFEAKPEEGDFEGAKAVFSFDPSDFVLTCFQRFDGGTARGDEETIKTVRSLFFRI"
     gene            17292..17846
                     /locus_tag="VFQ23_09620"
     CDS             17292..17846
                     /locus_tag="VFQ23_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9906890.1"
                     /translation="MSKIQTQGVHHITFVGSNREAIIDFYQGVLGMPLVFEQPNLDVP
                     DEMHLYFDPGDGRLITFFVRPTRVNDPTPNPQNIGNLHHIAYNVSRAIYTQVAARLKE
                     RGIENTGNIDRGFMDSIYFRDPNGQLLEMACFKFTPPGDHTYADVLSIAHRMRVEKGD
                     YAIADNHLADAIAELTEKKIKITA"
     gene            17843..18496
                     /locus_tag="VFQ23_09625"
     CDS             17843..18496
                     /locus_tag="VFQ23_09625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9906891.1"
                     /translation="MMVSADNDPRRHGTIWMMDLDKEIPAISPRIHADFRRVTLDLAP
                     ALAASTDSLTLTEITKRLESGRQCYAVWVDGQVVAYGWVSFEKEDIGELNLRIKLLPG
                     EAYIWDCMTLPAFREKLFYSAMLTYILSELRVQNLNRVWIGADLDNLPSQKGIARAGF
                     RHVADLIIERVLAMRQVWVTGLSDVPESIVAEARRAFLNDRDKVWLNAVSSTTQENR"
BASE COUNT         4789 a         4862 c         5008 g         4668 t
ORIGIN      
        1 ataagggtgt tgcggctgtc attatagaat gtttggaagc ggccgaagca cttggcatga
       61 ccgaatatgc ccgcgcccaa atgttgaaaa taatctctga cgaacctatg atagatcgct
      121 tcgtcagcgg cagcatcaaa catgccaaac gcagggtgga ggagatggaa gcagttgtcg
      181 agatgctcaa ttcaattagt gtttcctctt tcacgagtca agcctccatt aagagattaa
      241 aagaaattat ggagacaaga gaatgacaaa gaagattctc gtgatcggag cgcactctgc
      301 tgatttcgtc tggcgcgcgg cggggaccat cgcaatcatc acatctcagg gtggtagcgc
      361 caaggtcatc gcgctctcgt acggggagcg aggcgaatca ggagagttgt ggaaggaacc
      421 gaatcaaacc gtcgagaacg tgaagcggat tcgtcacgaa gaggcacagg cggcagcaga
      481 agcagttggc gcagagtttc aatgtctcga tctgggcgat tatccgctga acatgactga
      541 tggagcaact gagcaattga ttgacatctt tcaggatttt gcgcctaaca tcgtgctgac
      601 ccacaacccg gttgacccgt tcaatcctga ccacccagtt gcctctcaca tggtacagaa
      661 ggcgcggctc ttatcgaccg gcgcgggagt tgccagtgca ttcagtcgta tcactccacc
      721 tgacctatat ttctttgaac cgcatcagcc tgagttgtgc gacttcaagc caaacgtatt
      781 tgtggacatc acctctgtga tggacatgaa atccaaggca atggatgcga tgaaagcgca
      841 aggttacctg cataaatatt actccgagct tgccacgcgt cgcgctaacc atgcgcgcag
      901 aatctctggc aactccgaaa ttaagtttgc ggaagcgcat ttccgcacac tgccgtgggt
      961 ggtatctggt ttatgaaccc ccatgacctc catcgcttaa gtgaacttgg tgtggcaacc
     1021 gtgtatgaag cctcaggacg tgaaggcttg attgacatcc cactgatcca attgattccg
     1081 aacagtcggg ctgccggtcc tgcccgcacc gccgcatgtg gacaggatga caatctcatg
     1141 gtgcatgccg ttatcgaaca gattcagcct ggtgaggtgt tggtgctcgc catgcctgaa
     1201 ccggctccag tcgcgctcat cggcgaattg ttggcgacac aggtcaaggt caagcaagcc
     1261 gctgcgattc tggtagatgc ttctgttcgg gatgtgagcg agctcgtcga gctgggactc
     1321 cccatctgga cacatttcat ccgtgtgaga ggtgcgacaa aaaccaaaat tggcgaactc
     1381 aacacaaccg tcacggttgg tggaactcaa atctcgaatg gagatattgt cgtattggat
     1441 gtcgatggcg cggtttgcgt taaacgtgag cgcgtgtatg aagtactcag agcctctgaa
     1501 gaaaggctcg tcaaagaagc aagcctgcgc aaaaaattgt ccgcaggtga aacttcctat
     1561 gacttgcatg gtctgcgtgt ctatgtggaa ggaaacaaga aatgaccact caagctgaac
     1621 ccatcttcga cattgctcat ctcgcgcatg tggaattact cacgccgaag ttcaatgaga
     1681 gtttgcgttt ctttgtcgac gtgatgggca tgactgaaag cggacgagaa ggtgattcgg
     1741 tctaccttcg gggctgggac gattacgaac atcattcact gcaactgacg gctgctgcaa
     1801 aacccggctt gggacattat gccttacgtg cgacgagtcc gcaggcgcta caacgtcgcg
     1861 tggaagcgat tgagcagaca ggcttgggca aaggctggaa ggaaaacaat attggacatg
     1921 gtcctgccta ttgtttcacc gaccctgatg gacatcaaat tgaaatctat tacgagacgg
     1981 agtggtatca agctcctgcg gagctaaaac ctgctttgaa gaatcaggca tcccgttatt
     2041 ctgaccgagg cgtgggcgta cggcgcctcg atcaccttgc attgctggca gctgatgtgc
     2101 gctccaatcg tttgttcatg acacaaacgc taggtgggcg cgtgacagaa cagattgtgt
     2161 tcgatgacgg ttcagagaaa ggtgcgtggc tgacgttcaa taacaaatcg tatgatgtga
     2221 ccttcgctga agattcctac ggcgcaaagg gacgcctgca ccatttcacc tatgcgttag
     2281 atagccgtga agcaatattg cgcgcggcag acatttgtct tgagaatgga gtctttattg
     2341 aaacaggtcc acataaacat gctattcaac aaactttctt tttgtatgta tatgaaccgg
     2401 gcggtaatcg ttttgaaatc gccaacgccg gcgcgcgtct tatccttgca ccagattgga
     2461 aaccgattgt atggaatgaa gaggaacgca aaaagggaca ggcatgggga ttgaagacca
     2521 tcgagtcatt ccacaaacac ggcacgccgc ctgtggaatg aaaaggaaat cgcatgccac
     2581 acctaaccct tgaatattcc gacaacattg aagttgatgt cccgcttcta ttgacacgtc
     2641 tgcatgaaga agtggttgct acgggcgcca tcaatctgaa aggtatcaag agccgcgcca
     2701 tcgaacatac acaatatctc attgcagatg gtgaccctga gtatgcattt gtgcatgttg
     2761 ctttattgat ccgtgaaggg cgtccaattg aagtgcaaaa agatgcaact cagcgcgtaa
     2821 tgaaggtgtt aaagcaaact tttggacatc tctttgagac acgaaaatta tccctgacgg
     2881 tggatcttaa ggagatgcgt gatggtatcg ccctgacgga tcacaatatt cccgataagt
     2941 ccaaatcggg gtgaggtcaa ctggtaaaat tttgttgcga gccagccaga agcatgagat
     3001 tggacaaacc agaaacgata catgagaact caagttggca tcattggttc gggaccgtca
     3061 gggttattac tggcgcgcct cctgcatctg cagggcattg atacggtcat cctggagagt
     3121 cgcagtcgcg aatatgtgca gggacgcatc cgcgcgggcg ttatcgagca gggcatggca
     3181 gatctgctgc gagaagccaa agctggggaa cgcatggatc gcgagggact gattcacgag
     3241 ggcattaata ttgcatttga taaccgtctc gaacgaatcc attttccgag ccacacgaat
     3301 ggcaaggtgg tgatggtgta cgggcagacg gaaatcacac acgacctcat gaaagctcac
     3361 attgacgaag cggggggcat cgttcattat gagtccccag ccatccgcgt cgaggactac
     3421 aattctgact cgccacgaat catttatcaa cagaacgggg aacaaaaaga actaacctgt
     3481 gattttgtgg caggctgtga tgggtttcac ggcgttgccc ggcagagcgt gtcagcagat
     3541 aagatcaagg tttttgaacg aacatatccc gttggctggc tgggtatcct tgcagacgtg
     3601 ccgcctgtgc gggatgaact tgtttatgtg aatcacgagc gcggttttgc actttgcagt
     3661 atgcgctcac atacgcgcag tcgttactat attcaatgcc gccagagcga caaagttgaa
     3721 aactggacgg acgatgcatt ttgggatgaa ctgcgccgcc gccttcctcc acacgtagcc
     3781 gaggacgtga caactggtcc tcgcctcgaa atgagtgtta cgccgctgca tagttttgtc
     3841 gccgagccga tgcgcttcgg taggctcttc cttgcaggcg acgcaggaca tatcgtccct
     3901 cctacgggtg ccaaggggtt aaatcttgcg gcctcagata tttattacct ttctcgcgcg
     3961 ttactcgcct attacaaaga caaacgaacc gatttgcttg accgctattc cgagatcgcc
     4021 ctgcgccgtg tctggaaagc catccgcttc tcatggtggt ataccacgct gctccacaaa
     4081 ttcgatgaag accccatgac ctaccgcttg caaattgctg aacttgacta tctcaccggc
     4141 tctgctgctg ggcttacgac gcttgccgag aactacgttg gtttacctta cgagatcttc
     4201 gaatgaattt cgccaccctc aacggaacta atcttcacta tcgatctgat ggggacactc
     4261 agggtactcc gctcgttctc atccattcgc tcggaaccga cctgcgatta tgggacgacc
     4321 tgatcccaca tcttgatcca gccctgcggg ttgtccgcct cgatctgcgc ggacatggtc
     4381 tgtctgcgga cccggcggga gagttagctt tggttgattt ctcaaccgac atagcggctc
     4441 ttcttgacca tcttgaaatt gataaagcca ttattctcgg tatatccatt ggcggattga
     4501 tcgctctcga tttcgccttg cgaaatcagg aacgggtcag ttccctcatt ttggctgaca
     4561 cagccgccag aattggtaca accgaattct ggcagactcg tatggacacg ctccgtgctc
     4621 atggcatgga gcaccttgcg gattctatcc tcgcccgctg gtttgccccg gactttgcga
     4681 ctcgttgccc ggtacaattc cagggcttgc gcacattatt taaccgcaat tcggtagccg
     4741 gctacaacgc tgcttgcgcc gtactgcgtg acgctgattt gcggactgaa gtttcacaaa
     4801 ttacagtgcc agcgctggtc ctttgtggcg cgcaggatgc cgctactcca ccggatgtag
     4861 tgcaagagct tgccgagtct cttccaagtg ctcattttgc cgtcctgccc ggttccgggc
     4921 ataccccgcc gattgaacaa ccccagatca tggcagacca gatcaatgcc tttcttcgtg
     4981 cccaccgcac cgttggtgac atctatgaac agggaatgaa aatacgccgc gaagttctcg
     5041 gggatgctca cgtggatcag gctgaagtga acaaaactag ctttgacgcc gatttccaac
     5101 gcttcatcac agaaacagcc tggggcagtg tctgggcacg gccggggctg gaccggcgga
     5161 cgcggcatct gcttactctt gcgatgctgg cggcgctggg aaaggaacat gagtttgcga
     5221 tgcatgtgcg cgccagtcga aacaccggag tcacaatgga tgagatcaaa gaagtcctgt
     5281 tacaggtggc ggtgtatgct ggagtaccgg ctgcaaattc cgctattgct gctgccaagc
     5341 gggtcattgc tgaatttgag gagaaatagc catggcaaat agccaaccat tcaaacctct
     5401 ggataaagaa cgccatccac catatctcta cccgggatac aaatccaccg tgccgcgttc
     5461 gcccacgcgc cctctgattc cgcttcggca gagccttgcg gaactgaccg gtcctgttta
     5521 tggtttggat gccattcacc cgcttgacaa tgatctgacc cgaaacgccg tcaaagatgc
     5581 cgatccacag ggacagcgga tcattgtcac tggacgtgtg ctggacgaca cggggcgtcc
     5641 tataccgaat gcgcttgtcg aagtctggca agcgaacgcc gccgggcgct acctccacca
     5701 caacgaccag catgacgctc cgcttgatcc gaattttcat ggtgggggca gggttctcac
     5761 ggatggagag ggcatgtata ccttcacgac catacgcccc ggcgcatacc cgtggggcaa
     5821 tcacttcaac gcctggcgcc cggcacacat ccatttttcg atcatcggtg tcaactttca
     5881 gcagcggttg gtcacgcaaa tgtattttcc tggcgacccc ctcattcccc ttgacccgat
     5941 ctgcaacagc atcccagacg aaaatgcgcg tcagtcgctg gtcgcgacgt acgatcacga
     6001 gatcactcag ccagattggg cccttggtta tcgcttcgac atggtcgtct gtggtccgaa
     6061 gatgactcca tttgaatgac atgaatccac tccatcaaac tccttcccaa acagtaggtc
     6121 cctttttacg tatagggctg gttcacggcg aaaaacaaaa cgtccttgcg gacgaacata
     6181 tggctggcaa acatattcgt ttggccggcg ttgtgtacga tggaaatggc aagccaattg
     6241 aagatgctct cgtcgaaata tggcacgccg atgcgaatgg catttaccct caccctgttg
     6301 atccccgcca tagcgaagca gactctcact ttcgtggttt tggtagagcg gagaacgagg
     6361 atgggggatt gtatgagttc cacaccatca aacccggcgg gcgggacggg catgcccctt
     6421 tcatcaacct gcacctgttc gcccgtggaa tgcttctaca cgcgcaaacc cgcatttact
     6481 ttgcagatga acaggccaac gcgactgacc ccgttctcca atctgtccca gtagaacgac
     6541 gtgccaccct gctcgcccgc ctggaagaga acggcgagat tcctgtctat cgttttgaca
     6601 ttcacatgca gggcgatcag gaaacggtgt tcttcgagtt ggaataacca tgcccgcttc
     6661 cccttccgac tcacgcattt tttccccact gttcaacagc cctgctatgg ctgaaatttt
     6721 ctcggacgag caattcgttc gctgcctgtt gaaagtggaa ggttcgctgg cgcgggtaca
     6781 gggacagctg ggtgtgattc cggcaggcgc agcagagcag attgcatcga aggtaggaac
     6841 actttctgtg gattttgacc gactccgatc aggcaccgaa caggccgggt ttcccgttat
     6901 agaacttgtc cgccaactga aggctcatgt aggaaatgac ttcgccgatt acattcattg
     6961 gggtacaacg acgcaggatg tgatggatac tgcgctcgtc cttcaaattc gggcagccat
     7021 cgcgatcctg gaatctggac ttaaaacttt aattcaatcc ttggctcgtc tcgccgatca
     7081 gcaccgtcac accctcatgg ctgggcgcac acattcccag caggctctac ctatcccctt
     7141 tggactaaaa gttgctggct ggcttgcgcc acttatccgt cattgcgaac ggctctccca
     7201 actgaaaccg cgagtcctta ttctccaatt tggcggcgca gccgggacgc tcgcggcact
     7261 aggtaaagat ggtatcccgg tacggaactc gctctctgtt gaactcgaac ttgggatccc
     7321 ctccatgcct tggcatactc agcgcgattc acttgctgaa cttgcgaact ggttgtcact
     7381 ccttacaggc tcactggcaa agatggcgca ggatgtgatt ttgctctccc aatcagaaat
     7441 cgccgaagtg cgcgagagca gcgacgtagc tcgcggcggc tccagcgcca tgccgcaaaa
     7501 gagtaacccg gtcgtgagtg aattcattct ggttgcccac cgaacgaacg catccttgct
     7561 ttcatccctg caccaggcct tgcctcagga gcatgaacgc ggcacctatg ggcaaatgga
     7621 atggctcgcg ttgccgcaaa tgttcgctct cacgggttct gccatggaga aggcagcctg
     7681 gttgggtgag aatcttatcg tggacgcgga caggatgcgc gcaaacgtcg ccgcctcaca
     7741 gggtttgatg ttggcagaag cggtcagtta cgcgctcgcg ccgcatattg gcttcgcagc
     7801 tgctaaaaaa atggtttcag actcaattcg gcaagcagta accgatggac ggcacctact
     7861 cgatatcatc cgcgagcaga gccaggccat gctcgattgg gagtctttgc aggatgaagg
     7921 taactatctg ggcgcgtcgg aaacatttat tgacgcagta cttaaatcag cctcgcaaat
     7981 ataaaaacca ccccatttct caaatgttgt tgagagatga caacgtaaaa tcagacgctc
     8041 tcctctggaa acgatgtccg atcgacaaca aatatattgc gaagcctcca ctcgtaatcg
     8101 ccactctgta ggtgcaggaa tcaaatagtt tcttgcttag aggaacaccg cctagaggcg
     8161 cggcgagagc ctgcaacggc actcaaatgt atcccttcaa tattgttact tgttgccagg
     8221 tgatatttgc tcttccaatt gatcttttcg ccaggattca atatattcca gagattgatg
     8281 tcggaaatac gtgtcatata attctgcata atgaccgccc cgctccatca gcgaagtgtg
     8341 attcccttct tcgaaaatct gtcccttgcg caggacgata atacgatcag ctgatttgat
     8401 cgtggacagg cggtgagcga tcacgatcgc agtccggttt tggagaacaa catccagtcc
     8461 ctcctggatt tgagcctctg tgagaggatc gacactggcc gtcgcttcat cgagaatcag
     8521 aacagctggg ttctgcagca ggacccgtgc cagggcgaca agttggcgct gacccataga
     8581 aattcctttg ccctcctcac caacaggggt ctccagacct tgtggtagcg ctgccagcca
     8641 atcaccccca cctacacgtg atgccacggt cacgacctct tcatctgtcg cgtccggttt
     8701 tccatagcgg atgttctcag ccaccgttcc ggcaaaaaga aaaggcgtct gtgggacaat
     8761 tccgagttgg cctcgatatg aatcaagatt gaaagaacgg atatcctgac catcaatgag
     8821 cagctccccg ccctggaatt catagaaacg ggctaccagc ttacccaggc tcgatttccc
     8881 tgctcctgta tgtccgacca gtgccagagt ctctccggct tgtattgtca ggttgaagtc
     8941 tttcaacacg ttttcattct cggtataacg aaaatccaga tcccggaatt cgatgcgacc
     9001 ctgcaggcgc tgcgcaattt tatgatcgag ttgcaccacg cgcggctctg cgtcaattaa
     9061 cgcaaagacg cgttcactgg cggcgagacc cagctggaat tgactccaga aggaggcaat
     9121 gctgatcaac gggaaccaaa agagattgat gctctcgata aacagataaa gactcccagc
     9181 cgagatcgag ctggtgatca cgcttaatcc accaaagtag acgatgagag cggtgccaat
     9241 ccctgccact gtgcccagaa tgggaaaaat gctgctgaat gttaacccct gacgtaagcg
     9301 cagtcgatac gtttgcctgt tgaccggttt gaattcgtcg tagatacgct gttcctgacg
     9361 aaagttcttg gcgacgctaa ttccagtaat agcttcctga acattcgcat tcacctcggc
     9421 gagcacacgc tgggcttgag tggttgtata acgcgcgatg cggcggaaag ccagggcgat
     9481 gaacaccaca atcggtgcta tagccaacgt gattaaggcc aggcgcaggt tgatgaagat
     9541 cagtagactt acgacaatga tgacgatcag cacctggctc atcagattca gggtcaatgt
     9601 cactacgttc gaaaagtcct gggtatcaga ggtcacccgg ctgacgatct tgcctgagga
     9661 atactcgtca taaaacgaca tatcgcgcgc cagcacagca tcaaaggcat cctgacgtag
     9721 ttttagcacc acattcccga ccgtacgcgc ggtgtacttt tgacggaaaa agttgaagct
     9781 ccatgagagt acaccggaaa ctaaaatggc tgcaatcagc caggcggtgc gctgccagat
     9841 cgtgacgttt agatctgatg caccttctaa ttggtcgaga ctgcgtgcga tcaaaaacgg
     9901 gaacgcggca tccatgacgg aattcagcgt gatcatgatc gcaatcgtgc ccatcatcgg
     9961 gaagtgaggc ttgaagtacc cgataattct cgccaaaagt tggcggtcgg tgtattcgcg
    10021 atcatatgtc tcagcgtcca gaccatccat gatgaaaccc atacatttct ccttctaatt
    10081 tagctatacc tggcaaaaat ccggcgatag aggtcacaac gttgcatgag ctcttcatgc
    10141 gttccctgat cgacgatctg atttcgatcg agcaccagaa tccgatctgc ccatcggatt
    10201 tgagataagc gatgggtgat cagcaaagtg gtgcggccct cgagcactcg tctgatagca
    10261 cgctggatct ggtcttcggt ggcactatcg atggcgctcg ttgagtcgtc caaaatgagg
    10321 atgcgggggt ctgtgagcaa agcacgtgcg atggcgatgc gctggcgttg cccaccggag
    10381 agggtaaccc cgcgctctcc cacaatcgtt ttgtacccct tggtaaaagt ctgaataaag
    10441 tcgtgtgcct gggccgcttt ggctgcagat tcgatcgcgg cgtggtccgt ctgctggccc
    10501 aaactaaaag caatgttctc atcaatggga cgcgagaaca aaaagatatc ttgttcaatg
    10561 gtcgagatct gcgagcgcag cgaatcgagg ttccagtcgc gcacgtcaat cccgtcgata
    10621 acgatgcgcc cttcgtccac gtcatacgtg cggttgacga gcttggtcaa cgtggtcttg
    10681 cccgaaccgg tctggccgac gatggcaatg gtctctccgg gcgcggcacg gaatgagata
    10741 tctcgcaaaa cgggcgccgc atcggggtcg ttgtctaatc gaaatgtcac gtggtcaacg
    10801 atgatctccc ctttgagaac cccaatgtga ccggccttgt tctcatctac ttctgtctct
    10861 tctttcatca gatcgagaat acgctgagcc ccagcaatgc ccatttgcac cagtgaaaac
    10921 gtaaaaatgg agataaaggc cggaaagcgc aacacgccca ttagacccat ataggcaacc
    10981 agctcaccaa tggacagggc gttgcgtgag accaaccata ctccatgggc aaacgcgccg
    11041 ctgatggcga tgcccagcag aagaagagga agataacgcg cttcgatctc gccttgtttg
    11101 acaaatagat cacggactcg gcgagcgttc cctagaaatt taccccgctc ctgttcctcc
    11161 tgtacggccg acttgactac ctcaatccct gtaatggttt ctgttagccc ggcattcatc
    11221 tctccaaagc gccagcgttg ctcaccggcg acaggattga gttgattgac gtaacggcgc
    11281 agggcaaaga tatgggcaat cacaaagaga agaggtgccg ccagcaactc tgcgcgcaga
    11341 aacgcaatga agaccagcgg agaaataatg ccgatgctgg actcgatgat cagggaaacg
    11401 cctgggttca tcatcggatt gagctggcga acatcgcttg ttgcacgcga cataatgtcg
    11461 cccacccgtt ggcggttgtg gaaagtctgg ctcttcccca ggaggctgag atacaactcc
    11521 tcgcgagcat ctcgctccag gcgctggccg agcgtctcaa ccgaccatga gttggtaata
    11581 tccaacagcc cgcgtacgat caccagtccc agaatggtca ccgcaatggt gaacaggcga
    11641 gccgagctgg ggtcagggag caacgcctcg tcaaaagcca gaccgatgag tcttgggacc
    11701 gctgaaaaga gtatgttggt cagcgcggca gccagaagaa agctggcgag gaagtttgga
    11761 tagcggataa gatgggaaat aatccatcgc acagggctgg aacggttata aacatgctca
    11821 tccgctatgc tgaactcgcg tttggattgt gaaggcacag ctgcctgatt ttgcattaga
    11881 tcatcctcat gaattggtct ttgggagcgg gttggagaat atgtcagaga ttacgaacaa
    11941 tagtagcaca aatgttttac gttgaatatg cttaaaataa ccgcggaaga tttttggaca
    12001 aatctacaag aactctatgt caagaacgcg cgctataact tcctttcacc gatttagtaa
    12061 gccttgatta cgtactgttg tgctggtgag ttcatagata gttttccagc gaagaaactt
    12121 ctatgagcga cacttgtccg attaaatgga gcatcccaaa ccaatcactt tctgtcaaag
    12181 agcagatctg cagcttttct cgatgctcaa atctcagatc gagtagaact gttcttcaac
    12241 tgaaatgtgg ttcgggattg atcttcataa gcgcctcaca gggggacaag atgttccgcg
    12301 agttaaacag atctgtcaca ggataaaaaa gtatagaaaa tcttggcggt attgtaatcg
    12361 agttcaattg cggaaacgaa ttggtcggtc gataacaagt atattgtaaa cttccgctcg
    12421 tattcctcac tcattaagtg ccgcaattga ggggtttcta tattaatgaa ataccgccta
    12481 caggcggtga gaacacacag ggggattcaa atggtctcca ttcaataggc gagtatgtgt
    12541 gaataattaa atagccatca atgtaataac tctttgatga caccaaagcg atgcgaagca
    12601 atctttatgg tcagataaac tacattccga attgagtggc ctgtaggaac gattactggt
    12661 cgatctgttt tttcatttgg tccaggactg ttcaggcgga tttgacggcg cgtgcagaga
    12721 tccagttggt tgaattagca gatgcgttgc aataccaatc gaagttgaag atagagtcag
    12781 aggacatgga ttgtttttat aaatgtggtt aaaatatctg gagatcaaac ttggtttatc
    12841 ggttcaggag cctggtggat gtctgagaac atggtttgcc tccacattca actgatggga
    12901 tctgccgcaa tttctggagg ggaaatacca ctggtcctga atcaactcaa atcacggggc
    12961 ttactgtatt atcttgcagc gaccggggaa tctcattccc gcgaccatct tgcaacactg
    13021 ttatggggcg actcaagcca gagtgaggcc tatcattccc tgcgttccag tttgtatcat
    13081 ttgcgcaaat ccttgaggtc tgttaatgcg gaagaagcgt tgattagtga cggaggctcg
    13141 ataggactga gtcttgcctg gtatgaatgt gatgcaactg aatttgatcg cttattgaaa
    13201 caggggagtg aggcttccct gtcccaagct gtcgcactgt ataaaggtcc attcttacag
    13261 ggattttccc tctcggatgc gcacatgttt gaagattggg tgcagatgga aaatacgcgc
    13321 ctaactcatg cctgttctga agcattgagt cgattgacca cctttgcaga agaacgtgaa
    13381 gcgtgggaaa ttgccattgg ctatgttaac cggatgatcc gcattgatcc gctatccgaa
    13441 gcggcccaac aacgattgat gcgattgtat ctgggtcaag gggaagtggg gttggcgctt
    13501 cgtcagtatc gtcagttcga aaataggttg aaacaagaat tgggaattgc accgaatttt
    13561 gaaacccgag tgttgtacga ggatgccctt cgtcagcagg gaggcatcac aatcattccc
    13621 gcttctcatc acacttcaaa gctaacaccc aatgttttgc cttttgttgg gcgcgacgat
    13681 ttaattcatg agctatcaag tattagcgca gacattgaag caggacgcgg tgcgaccgtc
    13741 ttgattcagg gcgagggcgg aattggcaag agtcgtttga tccatgaatt tgcatcacga
    13801 ctgataacaa aatcacaacc ctggatgatt ctgcaaggct catgttcgcc ctttgatgat
    13861 ttactctcgc atggaccatt catcgaagcc ctgcaaaacg gatctttgga tgatctaaac
    13921 gatctccttg ccgagtcgaa tgccagtgtt cccgatgcac gcggccgatt cttctggcgc
    13981 gtgctgcaaa caattcgttc aatgagtaat aattcgccgc ttttgctttt cattgatgac
    14041 ctgcaatggg cgaatagctc aacactgaat ttatttggtt ttctttccat gcgcctccat
    14101 catctgcctg tgatgcttgt aggcacagta caacatgcag aagcaatccc cgccttgcaa
    14161 aggttgatca cgcttggacg tcgccgacat gaactccgtt tgctgtcttt gactccactt
    14221 acgcaagtag ccatcgcaga ccttttgcgc gcgtcaaatg tgaatccggg ttcgatagaa
    14281 actctggcag aatggctgaa tgccaaatca gctggcaatc catttttgct ttccgaaatt
    14341 cttgctcagt tgcgtacgga aataattctc aaagccacaa gcgatggctg gcaattggat
    14401 accacgcaat ggctgaggtg gcgtactacg ttttcgttgc ctgaaacagc tcacgatctg
    14461 gttggatggc gcttggcaaa tatttcacag gaggcgcgca gcctgctgga tgtgttagca
    14521 gttgcttctc agccaatgcc tgaatcggtg ctccgaaata ttcctggcgt ctggagcaac
    14581 ttatttcctg cgctcgtaga cgatttatcc gctcgcggac tcattatgga attgactggt
    14641 aattcatttc tcatgttgcc acatcatttg ttacgcgaga ctttattgca tcgcctgagc
    14701 aatctgcgcc gccgaaccct gcatcgccaa ctggcagagg caatggaagc gaaagcatat
    14761 tctgatccta atgtttggct ccgtcaaatc gcgatgcatg ctgttgctgg tgaagatgtg
    14821 aatcgggcgc atcattatgg gatgcttctc ctgccagacc ttccatatga atacacgggt
    14881 gcagagacca tcgactttgt gcaacacttg cacgatttgc tggctccgac agcctcacct
    14941 aatgaaatga ttcttatcac gcgtgctttg ggattgttgc atcaatcact tggtcatctt
    15001 gaaacggctg gtcaatggca tcggcagact cttgaatggg cacagaaaac gaatgattac
    15061 gcggcacaag ccgaagctta ttttgaaatg agcgaacttg cccttatgag cattgactat
    15121 cgcacagcga ggcagactgc tgaaaaaggt ctcgaagtaa ttcgtgcaac caggggtgct
    15181 gtgctattgg tccctaatgg taaaggtcat cgtttacttg gcgcgtcgct ggcaatggag
    15241 gggcgcgacc tggctggcgc cgaagaacat ctgcaaaagg ctgttgaaat ccatcgccag
    15301 attgaaaatc aaggcgactt gtgtgcggtg ttattcgaac tcggaaatgt cgccgcgcaa
    15361 cgcggggaat tgcaacgcgc cctcgatttt tatagtgagt ccgcgcatgt ggcagaggcg
    15421 ggacgaatcc attattatct ggccctggca cgcaacaact ttgcgtatca tagcctgttg
    15481 ctgggtcgcg tggatgaggc tgaacaatct gtttcccagg gcatcaagat ggcggaggcg
    15541 tatgatctac ttgcagcgct acttcatctt tacagtacta aaggcgaaat ccaattgcac
    15601 ctcaacaagt ggaatgaagc ggaagtctca ttccatagcg gacttgcact cgcagaggag
    15661 ctgggcagtc ttgaacgtca ggcaggttat cgcggcggac tggcgctggc tgcgcgaggt
    15721 aaaaaggatt ttgatatggc gcagcatctg ctcgaagaag cgcttgcttt gatagccgaa
    15781 cagggatatt ggcacctccg cacgcggctt caactttggc tcgcggagac gctctttgaa
    15841 caagccgact atgatgaggc aacaaaactt cttgacgaag ctatgcagat cgcccgctcc
    15901 cagcgccgct cgctattggt cgaacaaggg gaacaattac atgctcgact ccttgccata
    15961 agagaaaaat agaacctgcc tgataggtca acctgagcgt agcgaagggt ctctgtcaaa
    16021 atgtgtacga gactctcacc tgcacttgcg ccaggtgcaa gttgtcggtc gcaacgaacg
    16081 ctccctcaga gtgacatact gcaatgacgc tccagtgacg cctgctgact attattaaat
    16141 cagtctgggt ctaacctgag gctatgaatg gatttaatta cgtcgcttac gcccaggaag
    16201 tcatcttcgc catttgctca actcgcgttc cagaataaaa cgggacaaaa taatccgaag
    16261 ccaattgccg agctgactca acttgaaagt ttgcttcaag gtgcctggta gaaggaggtg
    16321 cagcgcaaaa tcagaaaaat tgagggatgt cttgaactat tgtcggccga aaccgtagac
    16381 aaaggagaaa taacatgccc cctgtaaatt ccatggacta tagcggtaaa gatactgtgc
    16441 tggatgtcgt tcgcactgat gcctcggaat tcttcaacat cgtggatgat ccgcaaaatt
    16501 ggaatgttca aacgcgttgt acggaatggg aagtacgcga tatggtcggt catatgctcg
    16561 acgtgacaga aggatacctc tcacgctggg agagcgcgcg taacggcgaa tcgcgcaaca
    16621 ccgcaggttt gcttgtgatg gctgactcgt tgaacaaggg cgcacagtcg tttcgcaacc
    16681 tgccgcgcac agaagccatc cagcgtctta aggacgatta cgccaaaatg atggaaattt
    16741 tccaaaacct gaccgccgac gaatggaata acttcatcgt cacacatccg tatatgggtg
    16801 ggttgcccac gttcttctac cctgcttttc atgtaatgga ttatggtgtt cacacctggg
    16861 atatgcattg gggactaggt gataaggatc gcaaattaga cgaacggact gctggcgtat
    16921 tgattcccta catgctatat gcccttctgc cttccacggt ggatcaacct tctgccaaag
    16981 gcgttgatgt agaatacggc atcgaagtct ctggggagtg gggtggcaag tggcgtgtca
    17041 ccgtgaagga tggcaagttc gaagccaagc cagaggaagg tgacttcgaa ggggcgaagg
    17101 cagtgttcag cttcgatccg tcagattttg tgttgacgtg cttccagcgc tttgacggcg
    17161 gcaccgcccg aggtgacgag gaaacgataa agaccgtaag gagtttgttc tttagaatat
    17221 agattcaaag aggccctaaa ggttttcgcg aagcgtacct ttagggtcta tcatgaaatt
    17281 ggagaaacta tatgtcaaaa atccaaacgc aaggggttca tcacattacg tttgtgggtt
    17341 ccaaccgcga agccattatt gatttttatc agggtgtgct tggcatgcca cttgtattcg
    17401 aacaacctaa tctggatgtg ccggacgaaa tgcatctcta ttttgatcca ggtgatggac
    17461 gattgatcac gttctttgtc cggccaacac gtgtcaatga cccgacgcca aatccgcaga
    17521 acattggtaa ccttcatcac attgcctaca atgtatcgcg tgctatatac actcaagtcg
    17581 cggcgcggct caaagaacgc ggcatcgaga acactggcaa catcgaccgc ggtttcatgg
    17641 attcgattta cttccgtgac ccaaatggtc aactgctcga aatggcgtgc tttaagttta
    17701 cgccgccagg ggatcacaca tatgcagatg ttctcagcat cgcgcaccgc atgcgcgtcg
    17761 aaaagggcga ttacgcgatt gccgataatc accttgcaga tgccattgcc gaattaaccg
    17821 aaaagaaaat caaaatcacc gcatgatggt ttctgctgat aacgacccgc gtcgtcatgg
    17881 gacgatctgg atgatggatc tggacaaaga gatacctgcg atcagcccac gcatccatgc
    17941 tgattttcgc cgcgtcactc tggatcttgc gccagctcta gccgcctcca cagattccct
    18001 cactttgacc gaaatcacca aacgcctgga aagcggacgt caatgctacg ccgtgtgggt
    18061 agatggtcag gtcgtggcat atggttgggt gagttttgaa aaggaagata tcggcgaatt
    18121 gaatctacgc atcaagctgc tgcctggcga agcgtatatc tgggattgta tgaccttgcc
    18181 agcctttcgt gaaaaactgt tttatagcgc gatgttgact tacatcctca gcgaattacg
    18241 tgttcaaaat ctaaatcgtg tctggattgg cgcagatctc gacaacctcc cttcgcaaaa
    18301 aggcatcgca cgcgcaggtt ttcgtcacgt tgcggatttg atcatcgagc gtgtactggc
    18361 aatgcgccag gtctgggtga caggcttgtc agatgtgcct gaatcaattg tcgccgaggc
    18421 acgacgtgca tttttgaatg atcgagataa agtatggttg aacgcggtgt cgtctacgac
    18481 gcaggagaat cggtgaatcg ccttttgcat agtgctgcat aatttacttt actttttatt
    18541 ggaatatcag cttgaaaatg ctgtgcaaaa gtaaagttat ttatgagaca agcggtcgga
    18601 atccgttgca gccggaaatt cgatccttac agttgtaccc tcatggggtg cacttgaaat
    18661 tgagatgctg ccattcattg cttccatcag agattttgcg attgagagtc ccaaaccaaa
    18721 cccaggaata gtagtgtagt catcgccacg gtagaaccga tcgaagacgt gttcaagctc
    18781 ttcgactgat ggaacgggaa gtttcccgga aggcggcgga tttcagcgag aacccggtgg
    18841 acttggtggc ggctttaact tattgataat ccatgaactg actgcttatg ctttattggt
    18901 cttagtcgcg ctacatattc ttgcgcgctg tatcatcaat ttttcctcaa agatcatctg
    18961 atctcaagaa tgtggtatgg aacagttgaa tagcgagcaa aacatccagg tttcagtttt
    19021 tatgatacta ggggaccgaa gcgcaaggat tggcttttcc cctggaatca aaccttaaca
    19081 tagacaaaaa ccgctcaaca gcatctcttg gcgggctctt tgaaattggt gccctcacgg
    19141 ggattcgaat accatcccct caatggggac gccagtcgta ctaaacaaaa aacgcctttc
    19201 ggcgatttct tgtgccccca cggggattcg aataccatcc cctcaatggg gacgccagtc
    19261 gtactaaaca aaaaacgcct ttcggcgatt tcttgtgccc ccacggggag acaccttgtt
    19321 ccgtgcc
//
LOCUS       DASTJP010000322         5737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_156858, whole genome shotgun sequence.
ACCESSION   DASTJP010000322 DASTJP010000000
VERSION     DASTJP010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156858"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..846)
                     /locus_tag="VFQ23_09630"
     CDS             complement(217..846)
                     /locus_tag="VFQ23_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906892.1"
                     /translation="MTIKIIIADDHPVVRSGLRALLSSQSDFEIVGEATNGEEAAKLA
                     ISLVPDLILMDLQMPILDGLGAIKLIRDKLPKANILVLTTYGTDADILPALEAGAIGY
                     LLKDTPPEQLFQAVRNVASGEMALAPRVAEKITQRLTSSSKNTLSSREIEVLELASQG
                     NPNKAIAKKLFITEATVKSHFVHIFSKMGVTDRTAAVTEALKKKIIKIE"
     gene            complement(836..2026)
                     /locus_tag="VFQ23_09635"
     CDS             complement(836..2026)
                     /locus_tag="VFQ23_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9906893.1"
                     /translation="MKIQKSINPEAWEQWDWVWHFSAYGLLILNIIFVYSSEPRITNF
                     SFFLILCVLLGLWYVPFLNISTLRIWDNPKRGALYLVPGWAIWAGLISLTADSLLLIG
                     IFFPLVFSRFPIRWATGITIFQTLGLYLLYIMLYPTERWFFVLLIILGLLTISTIIGA
                     FISSIIIQSTDRQRLIDDLTRSRANLMRVEREAGRLNERQRLARDIHDTLAQHFTSII
                     MHLSAAKHSNFGTVQTQVQQAEDAAREGLDEIRRIVWDIQPEQIEKASLIEAVEELAA
                     RWSAENSVQVKMNVTGTPRSLTSSAETALLRISQEAMHNINKHAQAKNVNITFSFMED
                     IFVMDIADDGLGFEPSTIKNGFGMRTMRDRAEELSGTLTIESEQGIGTAIAVSIPISE
                     ENDD"
     gene            complement(2102..2836)
                     /locus_tag="VFQ23_09640"
     CDS             complement(2102..2836)
                     /locus_tag="VFQ23_09640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9906894.1"
                     /translation="MNTTNHENSTVWRGVANETSTVWRRIAIVTLLLLVLVGPNFGPL
                     PHLLRESLSLEGSITGVLIWFWVAFLIILAVVYRMNKSTSIGEFLTSHGLGAPSRIGA
                     NIAGLILGIVWALLFLSSILQFDPDANITQINGFRILTALLAAGGALLEDLITRSFIM
                     NQLHKIGSSKWVQVVVSALIFALYHTIWGFNPFSFVFSVVYGLLLGGLFLWGKRSLTP
                     VILGHSLVVLISEPFATMLIFLVPGA"
     gene            complement(3167..3871)
                     /locus_tag="VFQ23_09645"
     CDS             complement(3167..3871)
                     /locus_tag="VFQ23_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HET9906895.1"
                     /translation="MSLSGLIPLARSAIMKLLLTSGGVTNTSIYDTLVDMLGKPIAEC
                     HALCIPTAQWGHPMCGPASVRGFIAAGSEFRYLSGLGWASLGVLELSALPTIGSERWV
                     PWLQEADVLLADGGDATYLCHWMRESGLADLLPSLPDTVWVGVSAGSMVMTPRIGAYF
                     VEWPSAPDDRTLGVVNFSIFPHLDAFPTNTLADAERWAADIGIPAYAIDEQTAIKVVN
                     GAVEVVSEGHWKQFTS"
     gene            4262..5512
                     /locus_tag="VFQ23_09650"
     CDS             4262..5512
                     /locus_tag="VFQ23_09650"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET9906896.1"
                     /translation="MKKGQDTRGYERYMALDIHREYILVGAWNEKKDWVLTPRRVSIE
                     KFPEWVKKNIRPGDIVVLETTTNVWTIYDIVAPLASRVLVANAAEVWELTGARVKTDK
                     EDIKRLLKLLVGGIVPEVWVPPVAVRELRAFISYRSRRVTTATMIRNRLQSLLHKHNL
                     YLSETGLLDQAWWEGQENISSLEKLQIRQELVLLAEVEKLKMAVDEELQRQSTSSQWG
                     KQAVQLMQLPGVGFVVAMTVLAAIGDISRFENAKQLVGYAGIGAGVHDSGKTHKDKKI
                     TKKGRRELRWAMVEAAWRAVRMSPFWKAEYEKHLKRMRKPNQAIVVIARKLLVAIWHV
                     LSKEETDIHASEEDLAYKMLLLSWSLSEDVRQGLTYRQFAKYALMKLGVENDITRFVR
                     KNVPRRLAPRDEVLARMTELGFSA"
BASE COUNT         1499 a         1340 c         1524 g         1374 t
ORIGIN      
        1 gaaggtcacg atatcttcgg attcaagcga gcgaaccgcc actccaccaa caggaagcgg
       61 gtcgataacc gcgaaataaa gaaagatcat cagcgcacca aagaagttcg cgccaaagat
      121 gagcgccacg agcctgcgag aaatgtctcg tgaagatggc tgggagatcg tcatggtacc
      181 tatcctcttc cctgcaaagg gtgtagattt caaaaactat tctattttaa taatcttctt
      241 ttttagtgct tcggttactg ctgctgtgcg gtcagttact cccattttag aaaagatatg
      301 gacgaagtga gatttaacag ttgcttcggt aatgaataat ttctttgcaa tggccttatt
      361 gggatttccc tgcgatgcaa gctctaacac ttcaatttca cggctgctca atgtgttctt
      421 ggaagaactt gtcaaacgct gcgttatttt ttctgctaca cgcggcgcga gggccatctc
      481 gccgcttgct acattgcgca ccgcttgaaa tagttgttcc gggggagtgt cttttagtaa
      541 ataaccaatt gctccagctt cgagagctgg caatatgtct gcatctgttc cataagtagt
      601 aagcactaaa atatttgcct ttggcaactt gtctcgaata agttttattg cccctaggcc
      661 gtctaaaatg ggcatttgca gatccatgag aattaaatct gggacgagag agattgccaa
      721 tttggcagcc tcttcgccat tggtagcctc gccgactatt tcaaaatctg attgcgagga
      781 tagcaatgcg cgcaacccac tgcggacaac tgggtggtca tcagcaatta ttatcttaat
      841 cgtcattttc ttctgaaata ggaatagaaa cagcaattgc agttcctatt ccctgctcac
      901 tttcaatggt taaggttcca cttaattctt ctgctctgtc ccgcatggtt ctcatgccga
      961 aaccattctt gattgttgaa ggttcaaaac ccaggccatc atcagcaata tccatcacaa
     1021 aaatatcttc cataaacgaa aaggtgatat tgacgttttt agcttgagcg tgcttattta
     1081 tattatgcat ggcctcctgt gaaatacgga gtagtgccgt ttcggcggat gatgtaaggg
     1141 agcgcggggt acctgtcacg ttcattttta cttgcacgct gttttctgca gaccatcgtg
     1201 ccgccaattc ttcaaccgct tcgatcagag aagccttttc aatttgttca ggctgtatgt
     1261 cccaaacgat tctgcgaatc tcgtcaagcc cttcacgtgc ggcgtcttca gcttgttgaa
     1321 cctgcgtttg gactgtccca aaattgctgt gctttgccgc agagagatgc atgatgatgg
     1381 atgtgaaatg ttgggcgagt gtatcgtgaa tgtcacgggc gaggcgctgc cgttcattca
     1441 aacgacccgc ttcccgttca actctcatta aatttgcccg ggaccgagtt aagtcatcaa
     1501 taagacgttg gcggtctgta ctttgtatta tgatggatga aataaaagca ccaatgatcg
     1561 tcgaaatagt cagcaaaccc aaaatgatca gtaagacgaa aaaccagcgc tcggttggat
     1621 atagcatgat atagagcaaa taaaggccca gggtttgaaa aattgttatt cccgttgccc
     1681 agcgaatggg gaagcggcta aatactaatg gaaagaatat ccctatcaac aaaagcgaat
     1741 cagcggtcag tgagatcaag ccagcccaga tcgcccatcc tggaaccaga taaagcgcgc
     1801 cgcgtttcgg gttatcccaa atgcgcaagg ttgaaatgtt taaaaatggg acgtaccaca
     1861 aacccaataa cacacaaaga atgagaaaga acgaaaagtt agtgattctt ggttcactgc
     1921 tgtatacaaa tatgatgttc aagataagca ggccataggc agaaaagtgc catacccaat
     1981 cccattgttc ccatgcctcg gggtttatgc ttttttgtat tttcattatc gcggcattat
     2041 accctgcgat ttccggcggt gttttgtccc ggaaatcgca gggtaatatc taaacgtact
     2101 tctaagcacc cggtacaagg aatattagca ttgtggcaaa gggttccgaa atgaggacca
     2161 caaggctgtg tcccagaatg actggagtaa gactgcgctt gccccataag aacaatcctc
     2221 ccagcaataa tccgtaaacc acactaaaga caaatgaaaa gggattgaag ccccagatcg
     2281 tgtgataaag tgcgaagatc aaggcggaaa caactacctg cacccatttt gaagagccaa
     2341 ttttatgcaa ttgattcatg atgaagctgc gtgtaattag atcttccagg agtgcgccgc
     2401 ccgccgccaa taatgcagtc agaatacgga agccattgat ctgagttatg ttggcatccg
     2461 ggtcaaattg aaggatgctg gacaggaata gaagcgccca tactattccc agtattagtc
     2521 ctgcgatgtt tgcgccgatt ctagaaggtg cacctagtcc atgggaggta agaaattctc
     2581 caatagacgt gctcttattc attctatata cgactgccag gatgatcaga aaagctaccc
     2641 agaaccaaat caggacgcca gtaatggaac cttcaaggga taggctctcc ctaagcaagt
     2701 gtggaagcgg gccgaagttg ggtccaacca gcaccagcaa tagaagcgta acaattgcga
     2761 tccttcgcca gacggtgctt gtttcatttg cgacccctcg ccagacagta ctattttcat
     2821 gatttgttgt gttcattttc ttcctctctc tttgctaaat tgattttgat aagtgaagtg
     2881 tagaacactt ttattcgggt gtcatcaaga gaaaggtgga tttattaatc aatcaaaagt
     2941 tggattaagt agagaagtgc ctgtggacgg gtgtggacaa aacttcgaga gcgggattct
     3001 gttcgggcgt agaaaaatgc ctgaaaatgg cgcagaatcc cacccgtcaa gtgcacgctt
     3061 tgttgagctt ttcgttggcc ggtttgctcg ccttcttccg ctgcaaggaa tcttgcgccg
     3121 cccctcgctc gtttggaata caaaatgaac aagtttccac acccaatcag gatgtaaact
     3181 gcttccaatg accttcagag acaacttcga cggctccatt gacgactttg atggctgtct
     3241 gttcgtcgat ggcataggcc gggatgccga tgtcggctgc ccaccgctct gcgtcagcca
     3301 gggtgttcgt cgggaaagcg tccaggtgcg ggaagattga gaagttgacg actcccaggg
     3361 ttcggtcgtc cggcgcggac ggccactcga cgaagtacgc tccgatccgg ggtgtcatca
     3421 ccatgcttcc tgcactcact cctacccaga ccgtgtcggg taacgaaggc agcagatcgg
     3481 ccagcccgga ctcgcgcatc cagtggcaca ggtacgtcgc gtcgcctccg tcagccagga
     3541 gcacgtctgc ctcctggagc cagggcaccc agcgctccga gccgatggtg ggcagtgcgg
     3601 agagctcgag gacaccgagc gaggcccagc ctaggccgga caggtatcgg aattcagacc
     3661 cagcggctat gaagcctcgt acagatgctg gaccgcacat cgggtgaccc cactgtgcgg
     3721 tcgggatgca gagggcgtga cactcggcga tcggcttgcc cagcatgtcg accagcgtat
     3781 cgtagatgct agtgtttgta acgcccccag aagtgagaag aagtttcatg atggcgctcc
     3841 ttgccaatgg aatgaggccc gaaaggctca tctagcttga ttgtacgaca atcaacgttt
     3901 ccaataaaaa ggaagatact atttcttctc agaaattttt tctgtgctat atctgccgcc
     3961 caacggtgag cgttattaga atgttcttgt ttgggacgtg gtaaccgcgt atccttgggg
     4021 tgattcatcg ccgggaacgc ggaagggacg agagcgtttg ggttgtccgc tcgtgggtat
     4081 gggaaccttc tcaccgcgaa tgagagggaa gcccgctgcg ttacccagca cctgcggtac
     4141 tgggcaggtg tatggacacc cggcggaaac taatctggga tggcgcaaag cgcctaagcc
     4201 cgcatcggta cctgcagctc aacacccgaa acccacgtcc caaacatacg aggagtctag
     4261 catgaaaaaa ggacaagata caagaggtta cgaaaggtac atggcactgg atatccaccg
     4321 ggagtacatt ctggtgggag catggaatga gaagaaggac tgggtcctga cgccacggcg
     4381 agtaagcatc gagaagttcc cggagtgggt gaagaagaat attcgtcctg gggatattgt
     4441 ggtgctggaa acgaccacca atgtgtggac gatctatgat attgtggcac cgttggcgag
     4501 ccgggtgttg gtggccaacg cagcagaagt gtgggagctt acaggagcca gggtcaagac
     4561 agacaaggaa gatatcaagc ggttgttgaa gctgctcgtt ggagggattg tgccggaagt
     4621 gtgggtgcct ccagtagcag tgagagagct gcgagctttc atttcgtatc ggagtcggcg
     4681 agtgacaacg gcaacgatga tccgtaatcg cctgcagagc ctgctgcaca agcacaacct
     4741 ttacctgtcc gagacaggat tgttggatca ggcatggtgg gaagggcagg aaaacatcag
     4801 ctcgctggag aagctgcaga tccgccagga actggtactg ttggctgaag tcgagaagct
     4861 caagatggca gtggatgaag aactccagcg ccagagcaca agttcgcagt ggggcaaaca
     4921 agctgtgcag ctcatgcaac tgcccggtgt ggggtttgtt gtggcgatga ctgtgcttgc
     4981 tgccattgga gatatttcac gttttgaaaa tgcgaaacaa ctggttggct atgcagggat
     5041 cggcgcaggt gttcacgaca gcggcaagac ccataaagac aagaagatta cgaaaaaggg
     5101 tcgtcgggaa ttgcgctggg ccatggtgga agcggcctgg cgggcagtgc ggatgtcgcc
     5161 cttttggaag gcagaatacg aaaaacactt gaaacgcatg cgcaaaccta atcaggcgat
     5221 cgtggtgatc gcgcggaagt tgctagtagc catctggcat gtgctgagca aggaagagac
     5281 ggacatccat gccagtgaag aagacctggc gtacaaaatg ctgctgctct cctggagcct
     5341 gagcgaagat gtccgccagg ggttgaccta caggcaattc gccaagtatg ccctgatgaa
     5401 gttgggtgtt gagaacgata tcacgcgctt tgtgcgcaag aatgttccga ggaggttggc
     5461 gccacgagat gaagtcctgg cgcggatgac tgaactgggt ttcagcgctt agtcgttgtt
     5521 tggatcgaca aaagagaagt ttttgaaccc catcacaaag caaaggctga cggggctggc
     5581 gaagtgttcc ctgaagaaga aaataattgt ctctgacaac caaaaaatct gccccaagag
     5641 caaagtctcg agcggcagat ttctttctca ccttgattca acggggtcac aggagtttta
     5701 tctccacact tgacaactgc agtaatcggt acccgct
//
LOCUS       DASTJP010000323        11712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_185259, whole genome shotgun sequence.
ACCESSION   DASTJP010000323 DASTJP010000000
VERSION     DASTJP010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185259"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..403
                     /locus_tag="VFQ23_09655"
     CDS             179..403
                     /locus_tag="VFQ23_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906897.1"
                     /translation="MALVSLFLAVLSIMSIVWLGRKHKERLAQLIVLSRWSNKEAGIR
                     VSISPDFGSSMIVVLATVYFALAVALVLTL"
     gene            676..1827
                     /locus_tag="VFQ23_09660"
     CDS             676..1827
                     /locus_tag="VFQ23_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HET9906898.1"
                     /translation="MYFTRQQLEENEDKSLAPYGMRSKDSKGRAYLDNEPEYRTAFQR
                     DRDRILHTTAFRRLEYKTQVFINYEGDYFRTRLTHTLEVAQIGRTLARALGGNEDLVE
                     AICLAHDLGHSPFGHSGEVALARLMKDFGGFDHNRQSLRIVTELEQRFPEFPGLNLTW
                     EVREGMVKHESEYDISDARDFSPELRGNLETQIANVADELAYTTHDLDDGLRSGMITP
                     HMLNGIALWEILRETYKWHGPHLDDIERHRMIRQLVGIMVTDMVESTDQRIRESKAKS
                     ALDIQRLKHNVIGYSEEMQRRNRELKDFLYKNLYRHYRVVRMQVKAERLISDLFSSYR
                     SEPLMLPTPAHRYIDKRGLERTVCDYIAGMTDRYAIEEHQKLFNPLEKP"
     gene            1843..2304
                     /gene="greA"
                     /locus_tag="VFQ23_09665"
     CDS             1843..2304
                     /gene="greA"
                     /locus_tag="VFQ23_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET9906899.1"
                     /translation="MAQQTYLTPEGEAKLKIELAELTGHKREELAQRLRSAIQMGDLS
                     ENADYHKAKEDQAFLEGRIQEIEAVLRTAIIVEKTNSDTVMVGCTVTIQEDNFPPETY
                     HVVGAKEADPRNGKISNESPIGKALMEHKVGDVVETDTPDGKIAFKILKIE"
     gene            complement(2364..3371)
                     /locus_tag="VFQ23_09670"
     CDS             complement(2364..3371)
                     /locus_tag="VFQ23_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906900.1"
                     /translation="MEAVTAIVRNLLQNSRVALILGVVLGLILGLIMGWGIWPVQWTD
                     GTPEVLRQDLQEDYLRMAIDSYKRTNDANMAVARYDNLGEAAGATFVRVQSNPGNINP
                     VDVQDYGLLIQSVKGAPITAGPAGGGATSGTSQLVIYASGIVILALLAFGGLYLFRLL
                     RRGPGTVTAVMQAAEASRQAEKTDFQSLGLAPPITQTMTTYVLGDDLYDESFSIDTGG
                     GEFLGEYGVGVSETIGVGEPKKVAALEIWLFDKNDIKTATKVLMSEHAYNDPNIRARL
                     EPKGELVVVKPQEQVLLETATLQLLATVVDMDYGSGAMPPKSYFERITLELAIWPRSG
                     Q"
     gene            complement(3498..4898)
                     /locus_tag="VFQ23_09675"
     CDS             complement(3498..4898)
                     /locus_tag="VFQ23_09675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906901.1"
                     /translation="MTTNQLINAQLPASFRDPSGFLFSRGGVLYRQVNRAYEQEYARL
                     FESGLYDKLVKAGLLIPHRETDQPPADPAVEMCFKVIQPEPVPFISYPYEWSFNQLKD
                     AALATLSIHRRALKAGLSLKDATAYNIQFRRGKPTLIDTLSFELYKEGQPWVAYRQFC
                     QHFLAPLALMALKDVRLNQLLRVYIDGIPLDLASELLPWNSRLNFGLLTHIHIHASAQ
                     KRYAGEEIKSRAGNTSMNKQAMIGLIENLEATIKKLNWTPQGTEWGNYYDITNYSDAA
                     FEHKKELIGAWVKRIKPELVWDLGANRGVFSRIAGEDGAYVISFDIDPAAVEQNYVLA
                     KNEKTENILPLLLDLTNPSPSIGWANRERESFGSRGPADMVFALAVIHHLAISNNVPL
                     PQMADFFASTGKWLVIEFVPKSDSQVKKLLASREDIFPNYTREGFESAFQEKFKIHES
                     MIVRESDRVLYLMEGK"
     gene            complement(4876..5052)
                     /locus_tag="VFQ23_09680"
     CDS             complement(4876..5052)
                     /locus_tag="VFQ23_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906902.1"
                     /translation="MFFAPLPYLDPGSGSFLLQLLIAALLGLGVAVRASWGKIKGWFG
                     IKPKPEEDDDESTD"
     gene            complement(5061..7379)
                     /gene="topA"
                     /locus_tag="VFQ23_09685"
     CDS             complement(5061..7379)
                     /gene="topA"
                     /locus_tag="VFQ23_09685"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET9906903.1"
                     /translation="MEAYCMKCKTKREMQDPVAGFNAKGSPVTIGVCSVCGTKLYRMG
                     RTPAHEGMIPPPKPPKVEKVEKRNGKLVIVESPAKAKTVGRYLGKGYSVRASVGHVRD
                     LLKSQLSVDVENNFTPKYRVPNEKKEVVKELKKLAQKAEEIYLATDPDREGESISWHL
                     LEAAEIDPERAKRVEFHEITESAIKEAFQHPRNINMDLVDAQQARRILDRLVGYGISP
                     ILWEKVRGRLSAGRVQSVALRLIVEREREIDAFVPVEYWSIHAEFKPDGLTSTFIAKL
                     AKIDEKDIELPNEETVKPILIDMETAAYSVTKVKRGERRRKPSAPFTTSTLQQEASRK
                     LGFTAKRTMALAQGLYEGQDVGSGGATGLITYMRTDSTNVSTSAQAEARQYVSGKYGK
                     DYLPPEPPQYKKRAANAQEAHEAIRPTSVMREPEQVKEYLEPAMYKLYRLIWQRFVAS
                     QMEAAVYDTLQVEVIGKSNAHEYLLRASGSSVKFAGFLVVYEEAKNEDVKTEEDEENV
                     KIPSGVAEGQKQELVRLIPEQHFTQPPPRFSEASLVQALEEDGIGRPSTYAPTISTIQ
                     QRGYVVREEKRLIPTEIGMQVNDLMVQYFPDIVDLTFTARMEEDLDMIANGQAEWAEV
                     MHEFYHPFADDLKKAQAEMPVTKSGPEPIGRICPEDGGELVIRYGRFGKFISCANFPN
                     CRYTEPWLEKIGVICPKDGGELVEKKTRKGRTFYGCANYPNCDFTSWKRPLKQPCPKC
                     NGLLVIANKREAQCTNCQEIFQLEEIVPETVE"
     gene            complement(7422..8513)
                     /locus_tag="VFQ23_09690"
     CDS             complement(7422..8513)
                     /locus_tag="VFQ23_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HET9906904.1"
                     /translation="MEHTYAVIMAGGGGTRLWPVSRKERPKQLLPLIGEETLFQSTVE
                     RLENLFPPERILVVTVEEQAREMLQQAPSIPKENYILEPAPRGTASVVGLAAAILQKR
                     DPDASMAILPSDHFIRNQDLFHYLLRAAFEVAENGYLVTLGITPTHPSTGYGYIQQGQ
                     PLSGGYKYPAYEVKSFVEKPDEEVAQKLLRLGNHSWNSGMFIWRADIILKEINKQMPS
                     LDEALKKISDAWGMPNQNEILKSNWHDLQNKTVDYGIMEKAEHVAVLPAGGLGWSDVG
                     SWQSLFEVLLPDMNGNIATNNSSHLAHQTTNTLVYGSSDERLIVTIGMDNMIIVDTDD
                     ILLVCKSDQSQSVRDIVDHLKKHRQEKYL"
     gene            complement(8518..9624)
                     /gene="dprA"
                     /locus_tag="VFQ23_09695"
     CDS             complement(8518..9624)
                     /gene="dprA"
                     /locus_tag="VFQ23_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET9906905.1"
                     /translation="MDDKKYWIGFNLIKGIGAVRMQALIQYFGDLEVAWKASPIELAN
                     AKLGKKLIERVIQARAGVDLDKLWDKIQKQGIKILTWQDETYPQRLKEIDQPPPVLYI
                     RGDYLPDDLFAVAIVGTRRVTAYGRQITEELSAFLAAQRITVISGLARGVDAVAHQTA
                     LKAGGRSIGVLGSGVDKIYPPEHRGMAEQMMQCGAIISDYAPGTPPEASNFPPRNRII
                     SGLSLAVVVIEAGETSGALITAEFAAEQGREIFAVPGSILAPQSKGTNKLIQNGALPL
                     LSVNDLMQALDLTRMGEQKAARKIIPADEIEARLLNVIGTEPLHVDEIRNQAELPIEK
                     VSAALALMELKGVVRQVGGMTYVSVREVESDYSV"
     gene            complement(9785..10321)
                     /gene="rimM"
                     /locus_tag="VFQ23_09700"
     CDS             complement(9785..10321)
                     /gene="rimM"
                     /locus_tag="VFQ23_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET9906906.1"
                     /translation="MAGKKNTSGSPDGEPVYLTVGFLRRPHGVQGEIMMDLHTDFPER
                     MKKGRKLFVGEEYQPMTLTNVRPHQAGLLVKFNGLETPEDAGKLRNQWVYIKAKDATP
                     LREGQIYQHELFGFRVVDENDNALGELVEIIETGANDVYVIRNESGKELLLPAIPSVI
                     LNLDTASRLMRVHLLEGL"
     gene            complement(10321..10554)
                     /locus_tag="VFQ23_09705"
     CDS             complement(10321..10554)
                     /locus_tag="VFQ23_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET9906907.1"
                     /translation="MKDLIEYIAKSLVEHPEEVQVRQSGGSRVRIELSVSKDDMGRVI
                     GKGGKVANSIRTLLRVAAEREGKQATLDVVEPQ"
     gene            complement(10601..10942)
                     /gene="rpsP"
                     /locus_tag="VFQ23_09710"
     CDS             complement(10601..10942)
                     /gene="rpsP"
                     /locus_tag="VFQ23_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET9906908.1"
                     /translation="MVRIRLRRIGLKGQPTYRIVAADKESPRDGRFLEILGFYNPRTN
                     PSTIHVKEDRAYHWMKNGALPTESVEKVFKSAGLLDRYERFKKGDAVEGLLKEAEEAE
                     AKRVEAIKAGK"
     gene            complement(11067..>11712)
                     /locus_tag="VFQ23_09715"
     CDS             complement(11067..>11712)
                     /locus_tag="VFQ23_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9906909.1"
                     /translation="LADVSHELRTPITVIQGNVEGILDGLYPADEERLKSIMEETQIL
                     SRLVDDLRTLALAESGALHLRREPVDLTELIREAVAGFESQAAEKDVQFILDLIELEE
                     INVDPQRVHEVLMNLISNALRYTPRQGVIKVGVVEANSGPVKSAVLFVEDNGPGIESA
                     DLPHVFERFYKSSDSGGMGLGLSIARYLVEAHGGKIWAESEIGKGTKISFTLSS"
BASE COUNT         2857 a         3072 c         2742 g         3041 t
ORIGIN      
        1 gctgccgcca aagtcggctg ttgaccgatt ggacaactga ttgagccaca aataaaatgc
       61 ccgtcatcct gatgggcatt ttttgtcctc gcaaaaatga taagcggatg aaaagcaacc
      121 gaaagttttc aacggaacaa cttaatatac aatattggca tagagaaagg atatcattat
      181 ggcgttggtt tctttatttc tcgcagtact ctcgatcatg agcatcgtct ggctcggacg
      241 caagcacaaa gaacgtttgg cgcagctcat cgtcctcagc aggtggtcaa ataaggaggc
      301 tggaatcaga gtgtctattt ctcccgattt tggatcgtca atgattgtgg tgttagccac
      361 cgtttacttt gcgttagcag tagcactggt actcacgttg tagcaggtac ccaccttgga
      421 aatgtgaaca aaagaccctc cgatctggag ggctttttgt ttatcctctt gactcgattc
      481 cgctagtgcg cttacacttc aaagacaaca gacgtaaaat ctgtcggtgg caacagcttg
      541 agggttcgaa gcgcttctcg ccttaccaac gaatgtcatt gcgaaccgcc gtcttgggtg
      601 gcgaggcaat ctcaggatac aatagattgc ttcgtcgtta acgctcgcaa tgacatttgc
      661 ttctagaaaa ttaccatgta cttcactcgc cagcagcttg aagaaaacga agacaaaagc
      721 cttgctcctt atgggatgcg cagcaaggac tccaaaggtc gcgcctacct ggataacgaa
      781 ccagagtatc gcacagcctt tcagcgcgac cgcgaccgta tccttcacac cacagcattt
      841 cggcggctcg aatacaaaac tcaggtcttt atcaactacg aaggcgatta ttttcgcaca
      901 cgcctgacgc atacgctgga ggtggctcaa atcggtcgca cgcttgctcg cgccctgggc
      961 gggaatgaag atctggttga agctatctgt ctcgcgcatg acctcggcca ctccccattt
     1021 ggacactcag gggaagttgc tcttgcccgc ctcatgaagg atttcggcgg gttcgatcac
     1081 aaccgacagt cccttcgaat tgtcacagaa cttgagcaac gttttcccga attccccgga
     1141 ttaaatctca cctgggaagt tcgcgagggc atggtcaagc atgaatccga gtatgatata
     1201 tcggacgctc gtgatttcag tcctgagctg cgcggcaacc ttgaaacgca aatagccaac
     1261 gtcgcggatg aactggcgta tactactcat gaccttgatg atgggttacg ctctggaatg
     1321 atcaccccgc atatgctaaa tggaattgcg ctctgggaaa tcctacgaga aacatataaa
     1381 tggcacggac cccacttgga tgatatcgaa cgtcaccgta tgatccgaca gttagtgggc
     1441 attatggtga cagatatggt cgaatcaaca gaccaacgca tcagggaaag caaggcaaag
     1501 tctgcactgg atattcagag actgaaacat aatgtcattg gctacagcga agagatgcaa
     1561 cgacgcaatc gggaactcaa ggattttctg tataagaatt tgtatcggca ttatcgggtc
     1621 gtgcgaatgc aagtcaaagc cgagagattg atctctgacc tgttcagttc atatcggtcc
     1681 gaaccgctga tgctgcccac acccgctcac cgatatatcg acaagcgagg gcttgaacgg
     1741 acggtatgtg attacattgc aggcatgaca gatcgttatg ccattgaaga acatcaaaag
     1801 ctattcaatc cacttgaaaa gccataaaca ggagttgcta ccatggctca acaaacctat
     1861 ttaacccctg aaggcgaagc caaactcaag atagagttag ccgagctgac cggtcataaa
     1921 cgagaagaat tggcgcaacg cttgcgctcg gccattcaaa tgggcgatct ttctgaaaat
     1981 gcagattatc acaaggcaaa ggaagatcag gcatttctcg aaggccgcat ccaggaaatc
     2041 gaagctgttt tgcgtacagc tatcatcgtt gagaaaacga acagcgatac cgtgatggtt
     2101 ggttgcacgg tcacaattca ggaagataat tttccacctg aaacttatca tgtcgttggt
     2161 gcaaaagagg cagacccgcg caatggaaag atatcaaacg aatcgcccat cgggaaagcc
     2221 ttgatggagc ataaggttgg agatgttgtg gaaactgaca cgccggacgg caagatcgcc
     2281 ttcaaaatct tgaaaatcga atagggtgat tacaatacaa aagagaccgg tgatcgccgg
     2341 tctcttttgt attaataatg aatttactgt ccgctgcgcg gccagatcgc gagctcaagt
     2401 gtgatgcgtt caaaataact cttcgggggc atcgctcccg agccatagtc catatccaca
     2461 acggttgcaa gcaactgcag tgttgcagtt tccagcaaga cctgttcctg cggcttgact
     2521 actacgagct cacccttggg ctccagccgt gcacgaatat tggggtcgtt ataggcgtgt
     2581 tcggacatta agactttcgt ggcagtcttg atatcattct tgtcgaacag ccagatctcc
     2641 aacgccgcca ctttcttggg ctcacccaca ccgatggtct ctgaaacacc gacgccatat
     2701 tctccgagga attcaccacc accggtatca atgctaaatg attcatcata taagtcatca
     2761 ccgagaacat acgttgtcat cgtttgggtg atgggtggtg ccaaacccag tgactggaaa
     2821 tcggtctttt cggcctgacg gctcgcttcg gcggcttgca tcacggcggt cacagttcct
     2881 gggcctctcc gcagcaatcg gaacagatac agcccgccaa aagccagcag ggcaaggatt
     2941 acgatgccag aagcataaat tacaagttga ctggtaccgg aagtcgcgcc accaccagca
     3001 ggtcctgcag taatgggtgc cccttttacc gactgaatga gcaagccgta atcttgaaca
     3061 tcaaccggat tgatattgcc aggattggac tgaacacgga caaacgtggc gccggcggcc
     3121 tctccgaggt tgtcgtaacg ggccaccgcc atattggcat cgttcgtacg cttatacgag
     3181 tcaattgcca tgcgaaggta atcttcctgt aaatcctgac gcaggacttc gggtgtgcca
     3241 tctgtccatt ggaccggcca aatcccccaa cccatgatca ggccaaggat cagacccaac
     3301 accacgccca agataagagc cactcttgaa ttctgaagta ggtttcttac gattgcagtt
     3361 actgcttcca tggcatattc cttttgttac cgcctgtctc acgcatgtag aacatgcaat
     3421 ttcaatcttt gaacgcgcgg cactttgtaa tgatgtctgc tcaattatat accacaatcg
     3481 aacggacagt atttcgacta cttgccttcc atcagatata aaaccctgtc tgactcgcga
     3541 acgatcattg attcatgaat tttgaatttc tcttgaaacg ctgactcaaa cccctctcgt
     3601 gtgtaattgg gaaaaatatc ttctctggat gcaagcaact tcttgacctg actgtctgat
     3661 ttcggcacaa actcgatgac cagccatttc cccgtacttg cgaaaaaatc cgccatttgt
     3721 ggcaaaggaa cgttattcga aatcgcgaga tgatgaatga cagccaaagc gaaaaccata
     3781 tccgctggac cgcgagaacc aaacgattcg cgttctcgat ttgcccaccc aatggatggg
     3841 ctggggttcg tcaggtctaa taatagcggc aaaatatttt ctgtcttctc attttttgct
     3901 agaacgtaat tttgttccac agccgcaggg tcaatatcga atgaaataac ataagcgcca
     3961 tcctcgcctg cgatacggct gaagactccg cgattggcac ctaaatccca aaccagctca
     4021 ggctttatac gcttcaccca cgcaccgatc aattcctttt tatgctcaaa ggctgcatca
     4081 gaataattgg tgatatcgta ataatttccc cattctgtac cctgcggagt ccagttcaat
     4141 ttcttaatcg tcgcttcaag attttcgatc aagccaatca tcgcttgctt gttcatgctg
     4201 gtgtttccgg cgcgggactt aatttcctct ccagcatagc gcttctgtgc gctggcatgg
     4261 atgtgaatat gggtcagcaa gccaaagttc aagcgcgaat tccagggcag caattcactg
     4321 gcaaggtcaa gcggtatgcc atcgatatag acacgcaaca gttgattaag ccggacatcc
     4381 ttcaacgcca ttaaagcaag aggagccaga aaatgctggc aaaactgacg ataggcgacc
     4441 cagggttggc cctctttata tagctcaaag gaaagggtgt cgatcagggt tggtttgccg
     4501 cgccggaact gaatgttata ggcagttgca tcttttagtg acagccctgc tttcagcgca
     4561 cgacggtgaa tggatagcgt ggcaagggca gcatccttca gctggttgaa cgaccattca
     4621 taaggatatg agatgaatgg caccggttcg ggctgaatga ctttgaagca catttccact
     4681 gccgggtcag cgggtggctg atccgtttca cgatgcggga tcaacagccc tgccttgacc
     4741 agtttgtcgt agaggcccga ttcaaacaat cgtgcgtatt cctgctcgta cgcgcgattg
     4801 acctgtcgat agagaactcc gccgcgagaa aacaggaacc cactggggtc ccgaaaggag
     4861 gccggaagtt gtgcgttaat cagttgattc gtcgtcatcc tcttcgggct tgggcttgat
     4921 gccaaaccat cccttaattt ttccccagga ggcgcgcacg gcaacaccga gacccagcaa
     4981 cgcagcaatc aacaattgaa gcaaaaaact gccggagcca gggtccaggt atggaagggg
     5041 tgcaaagaac ataatttctc ctactctacg gtttcaggaa cgatttcttc caactggaag
     5101 atctcctgac aattcgtaca ttgagcctct cgcttgtttg caatgacgag caatccattg
     5161 catttcgggc acggctgctt aagaggtcgt ttccaggagg tgaaatcgca atttggataa
     5221 tttgcgcagc cataaaaagt tcgtcccttg cgtgttttct tctcgacaag ctcaccgcca
     5281 tctttggggc agatgacgcc aattttttcc agccagggtt ctgtatagcg gcagttcggg
     5341 aaatttgcgc atgaaataaa tttaccaaaa cgtccataac gaatgaccag ctcgccgcca
     5401 tcctcggggc aaattcgtcc gatgggttca ggtccgcttt tcgtcacggg catctcggcc
     5461 tgtgcctttt tcaaatcatc cgcaaatgga tgatagaact catgcatcac ttctgcccat
     5521 tcggcctgac cattggcgat catgtccagg tcttcctcca tgcgcgccgt gaaggtgaga
     5581 tcaacaatat ccggaaaata ttgcaccatt aagtcattca cttgcatgcc gatctctgtg
     5641 gggataagcc gtttctcttc ccgaacgaca tacccgcgct gctggattgt ggaaattgtt
     5701 ggagcatagg tagacggacg accaatccca tcttcctcga gagcctgtac aagcgacgcc
     5761 tcagaaaaac gtggcggtgg ctgagtgaaa tgctgctcgg gaatcaatcg caccagttcc
     5821 tgtttttgtc cttccgccac gccggatggg atttttacat tctcctcatc ctcttccgtt
     5881 tttacatctt cgtttttcgc ttcttcatag accactaaaa agcccgcgaa tttcactgag
     5941 gacccagagg cgcgcaacaa gtattcatgg gcgttgcttt tccctatgac ctctacttgc
     6001 aacgtatcat agactgctgc ttccatttgc gacgcgacaa atcgctgcca gatcaaacga
     6061 tacaacttgt acatggcagg ttcaagatac tcttttactt gttcaggctc acgcatgacc
     6121 gaagtggggc gaatagcttc gtgtgcttcc tgtgcgttgg cagctcgctt cttatattga
     6181 ggcggttcgg gcgggagata atccttgccg tatttgccac tcacgtattg ccgtgcttct
     6241 gcttgtgcgg acgtagagac attggtcgaa tcggtacgca tgtaggtgat caaacctgtg
     6301 gcaccgccac ttccaacatc ctgtccttca tacaaacctt gtgcaagcgc catagtccgc
     6361 tttgcggtaa acccaagctt gcgcgaggct tcctgctgga gcgttgaggt cgtaaaaggg
     6421 gctgagggtt tccgtctgcg ttcgcctcgt ttgacctttg tcaccgaata agctgccgtt
     6481 tccatatcga tgagaatcgg cttgaccgtc tcttcgtttg gaagctcgat atccttctcg
     6541 tcgatcttcg ccagtttagc aataaacgtg gatgttaatc cgtcgggctt gaactcggca
     6601 tggatcgacc agtattccac ggggacgaac gcgtcgatct ctcgttctcg ctcaacaatc
     6661 agcctgagcg ccacagattg aactcgccct gcggaaaggc gaccgcgcac cttctcccac
     6721 aggatcgggc tgatgccgta accaaccaga cggtccagga ttcgacgcgc ctgttgcgcg
     6781 tccaccagat ccatgttgat gttgcgagga tgctgaaacg cttccttgat cgccgattca
     6841 gtgatctcgt ggaattccac gcgcttggca cgttccgggt cgatctctgc agcttcgagc
     6901 aaatgccagg aaattgattc tccctcacga tcagggtcag ttgcaagata gatctcttct
     6961 gccttctgag ccagtttctt gagttctttt acaacttcct ttttttcatt tgggacgcga
     7021 tacttcggcg tgaagttgtt ctccacgtcc accgataact gcgatttgag cagatcgcgc
     7081 acatgtccaa cggaggcgcg cacagaatag cctttaccga ggtaacgccc taccgttttg
     7141 gctttcgcag gcgactcgac gatcaccagc ttaccattcc gtttttcgac cttttcaacc
     7201 ttcggaggct tcggcggcgg gatcatgcct tcatgagccg gcgttcgtcc catcctataa
     7261 agttttgttc cacaaacaga gcaaacacca atcgtaacgg gagatccttt tgcattgaaa
     7321 cccgcaacag gatcctgcat ttcacgcttg gtcttgcact tcatgcaata tgcttccatc
     7381 aattctcctc cctcacctct ccaatatggg tgagggaaat attaaagata tttctcttgc
     7441 ctatgttttt tcaaatggtc tacgatatcg cgcactgact gcgactggtc cgatttgcaa
     7501 accagcagta tatcatctgt atccacgata atcatattat ccatgccgat tgtaacgatc
     7561 aggcgctcat cgcttgagcc gtaaaccaat gtattggtgg tttgatgagc caaatgactg
     7621 ctgttattcg tggcgatatt gccgttcata tctggcaaca atacttcaaa taaggattgc
     7681 caggacccca catcgctcca acctaagcca cctgctggca gaacggcaac atgttcggct
     7741 ttttccatga tgccgtaatc aactgttttg ttttgcaggt catgccagtt gcttttcaga
     7801 atctcattct gatttggcat tccccaggca tcgcttattt ttttgagagc ttcatccaac
     7861 gaaggcattt gtttgttgat ttctttcaaa atgatatccg cgcgccagat aaacatgcca
     7921 ctattccagg agtgattgcc caaacgaagc agcttttgcg ccacctcttc atccggcttt
     7981 tccacaaaac tctttacttc gtacgcagga tacttatagc ctccgcttaa cggttgaccc
     8041 tgttgaatat agccgtaccc tgtggatgga tgagttggcg tgatccccaa cgtcaccagg
     8101 tatccgttct ctgccacttc gaaggccgca cgcaggagat aatgaaataa gtcctgattg
     8161 cgtatgaaat ggtcggaggg caaaattgcc atgctggcat ccggatcgcg tttttgcaaa
     8221 atcgcggcag ccagtccaac cacggaggcc gttccacgtg gcgcaggctc aagaatgtaa
     8281 ttctctttcg ggatggacgg ggcctgttga agcatttcgc gggcctgctc ttcgacagtg
     8341 acaaccagaa tacgttcggg tgggaaaaga ttctcgaggc gttcgaccgt actctggaag
     8401 agtgtttcct caccgatcaa aggaagcagt tgtttgggtc gttcttttct tgagacgggc
     8461 caaaggcgag ttcctccacc tccagccatg attacagcgt aggtgtgttc catagtatta
     8521 gaccgagtag tcggattcta cctcacgcac cgagacgtaa gtcatgccac cgacctgcct
     8581 caccactcct ttcaattcca tcagagccaa agctgcagag accttctcaa tcggcagctc
     8641 agcttgatta cggatttcgt ccacatgcaa aggctccgtg ccgatcacat tcaagagacg
     8701 agcctcgatt tcatctgccg ggatgatctt tctagcggct ttctgttcgc ccatgcgggt
     8761 taagtctaat gcttgcatta agtcatttac gctcaataat ggcaatgcgc cgttttgaat
     8821 caacttattt gttcccttgc tttgcggtgc tagaatactg cccggaactg cgaaaatctc
     8881 gcgcccctgt tcggcggcga attctgctgt gatcaacgcg ccgctggttt cgccagcttc
     8941 gatcacaacg accgccaatg ataacccaga aatgattcga ttgcgtggcg gaaaattaga
     9001 agcctctggt ggcgttccgg gagcataatc gctgatgatc gctccacatt gcatcatttg
     9061 ctctgccatc ccacgatgtt ccggcggata gattttgtcg acacctgagc ccaagactcc
     9121 gatggaacgc ccgccagctt tcaacgcagt ttggtgagca accgcatcca cgccacgtgc
     9181 aagtccgctg attacagtaa ttctctgggc tgcgagaaag gctgaaagct cctctgtaat
     9241 ttgcctacca tacgctgtaa cccgccgtgt tccaacgatg gcaactgcga agagatcatc
     9301 tggcaagtag tcaccgcgta tgtataagac tggtggcggc tgatcaatct ctttcaaccg
     9361 ttgaggatat gtttcgtcct gccaggtcag gattttgatt ccctgttttt ggatcttatc
     9421 ccaaagtttg tccaaatcca cacctgcacg cgcctggatg acacgttcga tcagtttctt
     9481 acccagcttg gcattcgcca attcgatggg tgaagccttc caggccacct caaggtcacc
     9541 aaaatactgg atcaacgcct gcattcgcac ggcgcctatc cctttgatca agttaaagcc
     9601 aatccagtat tttttatcgt ccataaattt tagttatgta tcgttggtca agtaaattgc
     9661 gatgagcatc ccagtcctgg cgagaccaag gattaacgat caaatgaaaa cttcttccaa
     9721 gcgtatggtc tcgataaggg gcactagcct cagcccctac aagcaccgat ctattcaatc
     9781 gttgttacaa cccttcaagc aagtgaacgc gcatcaatcg actggcggtg tcaagattca
     9841 gaatgactga gggaatcgca ggtaaaagaa gttccttccc ggattcattc cttataacat
     9901 acacatcgtt cgcgcctgtc tcgatgattt ccacgagttc gcccaacgcg ttgtcgttct
     9961 catctaccac gcgaaatccg aacagttcat gctgataaat ttggccttcg cgcaaaggag
    10021 ttgcatcttt tgctttgatg tatacccatt gatttcgcag tttgcctgcg tcttcgggcg
    10081 tttccaagcc attaaatttt acgagcaatc cggcttgatg tggtcgtacg ttggtcaggg
    10141 tcataggctg gtattcttcg cctacgaaaa gctttctccc ttttttcatc cgttcaggga
    10201 aatccgtatg caaatccatc atgatttctc cctgcactcc atgtggacgc ctcaggaaac
    10261 caacagtcaa atagacaggc tcgccgtctg gcgagcctga ggtgtttttc ttaccagcca
    10321 ttattgtggc tcaaccacat caagtgtggc ctgttttcct tcacgttctg ccgctactcg
    10381 cagcagggtg cgaatggaat tggccacttt gccacctttg ccaatgacac gtcccatatc
    10441 gtctttagaa acgctgagtt caatgcggac tcgtgaacct ccgctttgcc tcacttgaac
    10501 ttcttccgga tgttccacca atgacttggc gatatattcg atgagatctt tcatttttta
    10561 ttcccatctc ccataagaac ggggctgatg tgaaggaaac ttacttccct gctttgatag
    10621 cttcaacgcg tttggcttct gcctcttccg cttctttcag gagtccttca acggcatcac
    10681 ctttcttgaa gcgctcgtaa cgatccagga gacccgcact cttgaaaact ttttcaacgg
    10741 attccgttgg caaagcgccg ttcttcatcc agtgatacgc acggtcttcc ttgacgtgaa
    10801 ttgtcgaagg attggtgcgg gggttgtaga aaccgagaat ttctaaaaag cgaccatcgc
    10861 gcggtgactc cttatcggca gccacaatgc ggtacgtggg ttggcctttg agaccaatac
    10921 gacgtaaacg aatacgaacc atcttacttt ttctccttag tttgttttgt gtctcaggca
    10981 ggcatagaga ggtgcgtgaa aggacgcatg cgggctgaaa tcacaggcag atattttacc
    11041 acgataaggg cggcagaacc tcacccctat gatgaaagcg tgaacgatat tttcgtgccc
    11101 ttgccgattt cactctccgc ccatattttt ccgccatgtg cctcaaccag gtacctggca
    11161 atggatagcc ccagccccat tccacccgaa tcgctggatt tgtagaaacg ctcgaagacg
    11221 tgtggcaaat cagcggattc gattcctgga ccattatcct ctacgaaaag aactgcactc
    11281 tttacaggac ctgaatttgc ttccacaaca ccgactttta tgactccctg gcgcggggtg
    11341 tacctgaggg cgttcgagat tagattcatc aacacttcat gcactcgctg tggatcaaca
    11401 ttgatttctt ccagctcgat caagtccaga atgaattgca catctttttc tgctgcttgt
    11461 gattcaaagc ctgcgactgc ctctcggatt agttcagtca gatcaacggg ttctcgtcgc
    11521 aagtgtagcg caccgctttc agctaacgcg agggtgcgca gatcatctac gagccgcgaa
    11581 aggatctgcg tctcctccat aatggacttc agccgctctt catccgcagg atacaaacca
    11641 tcgagaatcc cctctacatt gccctgaata acggtaatgg gtgtgcgcaa ttcatgcgac
    11701 acatctgcga gc
//
LOCUS       DASTJP010000324         4942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_136690, whole genome shotgun sequence.
ACCESSION   DASTJP010000324 DASTJP010000000
VERSION     DASTJP010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136690"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..2444
                     /locus_tag="VFQ23_09720"
     CDS             447..2444
                     /locus_tag="VFQ23_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9906910.1"
                     /translation="MAKKLLQSLFILVIWTSLTNNNALAVTGDVYVSPQGNDSGACTS
                     GAPCRTIQKGVNTVQPGGVLHILAGTYAESVTVSKTGTPSAPIRIVGEGAVLSNGGAR
                     AFYVNNSQWVTFEGLKIRGYTSMDFEIKQSHFLTFRNNSLEYTFAALRIQEGVSHVLV
                     EYNEMYQTYPASSTWSSLKGSRYEGAGVYASSGAQGMYFIRNNNFHDSMNGVYLSDSD
                     AGLWMNANVFISGNTFRNIVDDPFEPEGDSFNIHFYNNTLINTHRIVSIVPASTCIGP
                     IFVYGNFQQNTLDPTREASSGRRNSAIKMDMRGGSCPNGVWFFNNTVNANAAGTNFYA
                     VDLLSTAVRNFQLLNNVFITEKNAYSSTPSLTNAVFDYNISLKPFGYAEPHGLQADPL
                     LNSSGMLQSNSPAKGRGTSIGVNSYFASAQIVPAGSDLGGFRNFPSPVYVLPPGGEPA
                     SFPTNASGWPNSLPPTSTPASPTPSPMVSPTLTQTVTSTPILPTNTATPTLTQNSTFT
                     APTFTPTSVLTSTPLPLPTTTAPPPTATRTAPPAGVIETIHDDNNPALVYSGNWQSTS
                     RDKAYGSSFKVTRQVGASITMSFTGQSFSLLYTSGDTNGKIDIYVDNVLIGTLDQRTS
                     TVLFQQRWDTSVRFTAGAHQLKLIFTGPADTRGAIDAVIIR"
     gene            complement(2538..3317)
                     /locus_tag="VFQ23_09725"
     CDS             complement(2538..3317)
                     /locus_tag="VFQ23_09725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906911.1"
                     /translation="MENLFLNYDHIAPQYNQRYPSSQLSERGQILLELAKKVEARNIL
                     EAGSGTGIWLNMLRSVTGGLYGLDYSMGMIRQAQMQPSSLKLARGSAIRLPYQNDTFD
                     LVYCVDAIHHFVDHQAFVAEAFRVLRPGGALAVIGFDPHDGATHWYIYDYFDGVYETD
                     LRRYPSGTSLLAWMQRAGFQNVSSQLAEHILNVHAGDAVLKDPYLKHTSTSQLALLSQ
                     SAYQAGIERIKAAIVAARTRDEKIVFRSDIPVMAYLGYKPE"
     gene            complement(3616..4041)
                     /locus_tag="VFQ23_09730"
     CDS             complement(3616..4041)
                     /locus_tag="VFQ23_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9906912.1"
                     /translation="MIRSLAHLNFVTNDLSKIIDFYVDKLGLKVKFTLDNKEGEPFGY
                     YFECGNTTFLEFFDQAMASKVWGGNVEELTIGNRYRHFCLEVTGLDEYCTDLKSKGVE
                     VSEIRMGMDNARQAWISDPDGNQIELMEYGPSSLQLAGR"
     gene            complement(4065..>4942)
                     /locus_tag="VFQ23_09735"
     CDS             complement(4065..>4942)
                     /locus_tag="VFQ23_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906913.1"
                     /translation="TVTSTPRSTFTPEPSVTPFQTFILPTPTRIQRIQYFRVKHDNQL
                     AMFNYKSRTADKAWPGRDQTPEVVPLFGSPGTSASTNRTRLVGTWENLINALNGNDEG
                     KLRYLKAPHTGLFNTAGFPQLESLTMGGNIVILDEIRGEWGRVRTLDYNNPPDLVGVT
                     YTTRPDLIHKFVVVGWRQDSKTTILVNPPPGSLYWPLVSKDPVWIELSRLEKFPDLPM
                     TVTVNDTLYIQQTPGPANDPTDRQLDAGETARIVEYFPSASAVWGRLLGGGWVTLHWQ
                     RQYPTTWKMETVPPP"
BASE COUNT         1274 a         1247 c         1181 g         1240 t
ORIGIN      
        1 ttgccgaaag cctgtttttt aaccgcttcg atcaatccct tgcggctctc ttcggctgcg
       61 ctcgagccaa ctacattggt tcgccaaacc gcaactgcgg tcgttaatga aacgatggcg
      121 atcaggattg cattgacaac ttcaaatcgg gaggtgaacc actttgatct gttcataagt
      181 gctccatcag agttcagcgt gtttgatgcg gcgagtatag catacccatt aatgaacggg
      241 aaaaacccac ctgttaagca cggatgccgc aaagcacctt gcagagttgt tatccaaact
      301 caaatacacg ataaatgacc atgaccattg gttgaggctt acagatatgt gaatggattc
      361 gttcatctat tgcttcattg atccttttgg tctgcataac atagtgtgag tatgcagcaa
      421 gacaacttca ctgcgtagga gaaatgatgg caaagaagct attgcagtcc ctcttcatcc
      481 tggtcatttg gacctctcta acaaacaaca acgccctcgc agtcactggc gatgtgtatg
      541 tttcaccaca aggcaatgat tcaggtgcat gtacatccgg tgccccttgt cggaccattc
      601 aaaaaggcgt aaatacggtt caacccggag gcgtgttgca catcctggct gggacgtatg
      661 cagagagcgt gaccgtttca aaaacaggaa cgccatccgc gccgattcgt atcgtcggtg
      721 agggagccgt attgagtaac ggtggtgccc gagcctttta tgtgaataat tcacagtggg
      781 tgaccttcga aggcttaaag atcagaggct atacctcgat ggattttgaa atcaagcaaa
      841 gccatttcct gacctttcga aacaacagcc ttgaatatac gtttgcggcg cttcgcattc
      901 aggaaggcgt gagtcacgtc ctcgtagaat ataacgagat gtaccagacc tatcctgcaa
      961 gcagtacatg gagttcatta aaaggctcaa gatacgaagg cgccggcgtt tatgcaagtt
     1021 caggtgcgca gggaatgtat ttcatccgaa acaacaattt tcacgactca atgaacggcg
     1081 tctatttatc agattcggat gctggtctgt ggatgaacgc gaacgtcttc atcagcggca
     1141 acaccttcag aaatattgta gatgatccgt tcgaacctga aggagattct tttaacattc
     1201 acttctacaa taatacgctg atcaatacac atcgtattgt aagtattgtt cccgcctcga
     1261 cgtgtatagg tcctattttt gtgtatggca actttcaaca gaatacactt gaccccacga
     1321 gagaagccag cagcggacgc agaaattcag cgatcaagat ggatatgcgt gggggcagct
     1381 gcccaaatgg tgtctggttc ttcaacaata cagtgaatgc caatgcagcc gggacaaact
     1441 tctatgccgt tgatttgctc agcacagccg taagaaattt tcaactactc aataatgtat
     1501 tcattacgga aaagaatgcc tactccagca cgccgtcctt aaccaatgcc gtatttgatt
     1561 acaacatcag tctcaaaccg tttggatatg cagaacctca tggtctgcaa gcggatcccc
     1621 tgctcaattc cagcggtatg ttgcagtcta atagtccagc caagggacgc ggcacgtcga
     1681 tcggcgtcaa cagctacttt gcctccgcgc agatcgtccc cgcgggaagc gacctgggtg
     1741 gattccgcaa ctttccatcg cctgtgtatg tgctgccacc aggcggtgag ccggcgagct
     1801 ttccgaccaa tgcatcaggg tggccgaatt cgctcccacc aacttccaca ccagccagcc
     1861 caacgccttc gcccatggtt tctcccacct taacgcagac tgttacatcc actccgatct
     1921 tgccgacaaa tacagcaacc ccaacgctga ctcaaaattc aaccttcaca gcccctacct
     1981 tcacacctac cagtgttctg acttccactc ctttacccct acctacaacc actgcgcccc
     2041 cgccgacggc gacgagaacc gcgcctcccg caggagtaat cgagacgata cacgacgaca
     2101 ataatcctgc cctggtttat tcaggcaact ggcaatcaac ctcgagagac aaagcctatg
     2161 gaagctcgtt caaagtgacg cgccaggtgg gcgcgagcat tacgatgagc tttacagggc
     2221 agtcattcag cctgctgtat accagcggag ataccaatgg caaaatcgat atttatgtgg
     2281 ataacgtttt gattggaacg ctggatcaaa gaacgtctac agtcctgttt cagcaaagat
     2341 gggatacttc ggtaagattc acggctggag cgcatcaact aaaactgatc ttcacgggtc
     2401 cggctgatac cagaggcgcg attgacgcgg tgatcattcg ataaggaata aacaggttgt
     2461 catcggtgcg cgttttcgcg accaattcaa ccattcaaat tcggccgatc atttttctgc
     2521 ccaacgattg aaagtatcta ttcgggctta taccccagat atgccatcac cggaatatct
     2581 gatcgaaaga cgatcttttc atcgcgtgtt ctggctgcga caattgcggc ttttatcctt
     2641 tcgatgccgg cctgatacgc actctggctc agcaatgcca gctgcgaagt ggaagtatgt
     2701 ttcaggtacg gatccttcaa gactgcatcg ccagcatgca cattcaaaat atgttcagca
     2761 agttgagaag acacattttg gaatcccgcg cgctgcatcc aggcaagcag cgacgttccc
     2821 gatggatacc ggcgaagatc cgtttcgtag accccgtcga aataatcgta gatataccag
     2881 tgagttgcgc catcatgcgg atcaaaaccg atgaccgcaa gcgcaccgcc cgggcgtaac
     2941 acgcgaaaag cttcggcaac aaacgcctgg tggtcgacaa aatgatggat cgcgtccaca
     3001 caatagacga ggtcgaaggt atcgttttga tatggcaggc gaattgcaga gcctcgcgcg
     3061 agtttgaggg aggacggttg catctgcgcc tgacgaatca tacccatgga ataatcgaga
     3121 ccgtacaacc caccagtaac cgaacgaagc atattcaacc agatgcctgt gccgctccct
     3181 gcctccagaa tgttccgggc ctcgaccttt ttggctagtt caagcaaaat ttgtccgcgc
     3241 tcgctgagtt gggaggatgg gtaacgctgg ttgtattgcg gcgcgatatg gtcatagttg
     3301 agaaataaat tttccatgcc ttactgaacc aaccagacca gattttctta cgctcagaaa
     3361 gcaaaaacct cggtggtcaa gtttcaaaaa gcgatgggat acttcgggta gactcgctgc
     3421 gggaacatat caacgaaaac tggtctttac cagtcctgct gattccagag gcacgatcga
     3481 tgcagtgatt gttcgttagt agaaaagtga ttaactaatt ttatgaagtc tagccacaga
     3541 gcacgcagag aattcccttt gtttttctct gagactctgc atctctgtgg caaaattgtg
     3601 aaaggcgagt ccgacttatc gacctgcaag ctgtaaactg gaaggaccgt attccatcaa
     3661 ttcgatctgg ttgccatccg ggtcagagat ccaggcttgt cgagcgttat ccattcccat
     3721 cctaatttcg gaaacttcca cacctttgct tttcaagtcc gtacaatatt cgtccagccc
     3781 tgtcacttca aggcaaaaat gtcgatagcg attgccaatc gtgagttctt cgacgtttcc
     3841 accccatacc tttgacgcca tcgcttgatc gaaaaattca aggaaggttg tgttgccgca
     3901 ttcaaagtaa tacccaaacg gctcgccttc cttgttatcc aaagtaaact tgacttttag
     3961 tccaagttta tcgacataga aatcaataat tttggaaagg tcgttggtga caaagttgag
     4021 gtgtgccagt gatcggatca ttttgattcc ctttttgcag ttatttatgg aggcggtacg
     4081 gtttccattt tccaggtggt tgggtattgt ctttgccagt gtagggtgac ccagccacct
     4141 cccaacaatc ggccccacac cgccgaagcg gatggaaaat attcaacaat tctagctgtc
     4201 tccccagcat ccagttgacg gtcggttggg tcattcgctg gacccggagt ctgttgaata
     4261 tacaacgtat cattcaccgt aacagtcatc ggcaggtctg ggaatttttc gagcctgctc
     4321 aattcaatcc agacgggatc tttggacacc aggggccagt ataaacttcc gggcggaggg
     4381 ttaacgagga tggttgtttt ggagtcctgt ctccaaccta caacaacgaa tttatggatc
     4441 aggtctggcc gggtcgtata agtaaccccg accagatctg gtggattatt gtaatcaagg
     4501 gtacgcactc taccccattc gccacgtatt tcatccagga tcacgatatt cccacccatg
     4561 gtgaggctct ccaactgggg aaacccggct gtgttaaata gaccggtatg gggagccttc
     4621 aaatacctca atttgccttc atcattgccg ttcaaagcat tgatcaaatt ttcccaggtt
     4681 ccgaccagtc ttgttcggtt cgtggaagcg gaggtgccag gagatccaaa tagcggtaca
     4741 acttcaggcg tttggtcccg accgggccaa gctttatccg ctgtgcgcga tttgtagttg
     4801 aacatggcaa gctgattgtc gtgtttgacg cgaaaatatt gaattcgttg aatacgagtt
     4861 ggtgtgggga ggataaaggt ctggaacggg gtgacgctgg gctcaggtgt aaacgtgctg
     4921 cgcggcgttg aagtgaccgt tg
//
LOCUS       DASTJP010000325         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1068601, whole genome shotgun sequence.
ACCESSION   DASTJP010000325 DASTJP010000000
VERSION     DASTJP010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068601"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1430)
                     /locus_tag="VFQ23_09740"
     CDS             complement(69..1430)
                     /locus_tag="VFQ23_09740"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9906914.1"
                     /translation="MSESAQFEVHDRDVLANARSILSATESPRQWAQAVIDAVKRNEE
                     WRGERCLNLLAPEAPTSPMVRALLSSEIGTRAAEGNIGRINRWFAGTQHIDEVEALCV
                     ELLKVAFRCNYADHRLMGSMLGNMTVFHALTQPGDVIMSAPQPFGGHSSNRYDGPAGT
                     RGLKIFDVPFNMDELEVDLDAFAKVARQHKPKLVVMGMSMTLFPLPVREMSQILSEWG
                     GQFIFDGAHQAGLIAGGQFQDPLNEGAVILTGSAGKTFSGPQSGMILWNDPNLTKPIT
                     ETIFPALAATHQVNRVAALAVSAAEMIEFGKFYMAQIVKNSKALAKALDERGIPVLCA
                     HKGYTQSHQVIARVKQYGGGLEVAHRLAKANIITNKNLVPEDKPEDWDHPSGLRIGTI
                     EVTRLGMNEKDMETIADFIARVLVKNEDPETVGKDVVDFRYPQQTLYYNFDNEYPTWA
                     KVK"
     gene            complement(1464..>2478)
                     /locus_tag="VFQ23_09745"
     CDS             complement(1464..>2478)
                     /locus_tag="VFQ23_09745"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9906915.1"
                     /translation="STCTITGTADVASNTLFLDIWKPAAESYGGGIFLQEVTDAKIYD
                     NDLQHQMNGLLSYQCQRLDVARNLANYCSGFGFHLFETSDSTFTENYADYCCRYYLSS
                     SSSHLGADATGFLIIYKSCNNVFRRNNARLGGDGFFLAGLTPDGIDVGCNNNLFEEND
                     ASYSPNNAFEGVFSKGNIYQRNKANHSNYGFWLGFSSDCTLEENQIYNNRQAGVAVEN
                     GVGFRVIQNDIQNNTHGILIWTRFYDFLKKVHGVNQTSQDWHIERNKLFQNKKAIRIA
                     ANQNHGTRALDAELSPITPNNHTIYNNEIRGNVIGIELEKTKRTRLNQNLLDNLIADF
                     DDK"
BASE COUNT          552 a          621 c          607 g          698 t
ORIGIN      
        1 tgggtgtaga tgtgtgcatt gtggaggagt ttcataggtg aatttatttt aattcagttg
       61 gcgaaatctt attttacctt cgcccaagtt ggatattcat tatcaaagtt gtaatacaag
      121 gtttgttgtg gatagcgaaa atcaaccaca tcttttccaa ctgtttccgg gtcttcgttc
      181 ttcaccagga cgcgcgcaat gaagtctgcg atggtttcca tatccttttc attcatgcca
      241 aggcgcgtca cttcgatggt gccaatgcgc agtccgctgg gatgatccca gtcttcgggt
      301 ttatcttcag ggaccaggtt cttgttcgta atgatattgg ctttggcgag gcggtgcgcg
      361 acttcgagtc cgccgccgta ttgtttgaca cgcgcgatca cctgatggct ttgagtgtag
      421 cccttgtgcg cgcatagcac cggaatgccg cgttcatcca atgctttagc aagcgctttg
      481 gaattcttca cgatctgtgc catgtagaat ttgccgaact ctatcatttc tgcggcagag
      541 acagcgagcg ccgcgacgcg gttgacctga tgggtggctg ccagggccgg gaatatggtc
      601 tccgtgattg gctttgtcag atttgggtca ttccacaaga tcatgccact ttgtggaccg
      661 ctaaaggtct tgcccgcgga tccggtcaaa atcactgcac cttcgttcaa gggatcctga
      721 aactgaccac ctgcgatcaa acctgcctgg tgcgcaccgt cgaagataaa ctgcccgccc
      781 cattctgaaa gtatctggct catctcacgc acgggcagcg ggaatagtgt catagacatg
      841 cccattacga ccagtttcgg cttatgctgg cgggcaactt tcgcaaacgc atcaagatcc
      901 acttcgagtt catccatatt gaatggcaca tcgaaaatct tcaagccgcg tgttccggcg
      961 ggtccatcat atcgattgct ggaatgtccg ccaaacggtt gcggtgcaga catgatcaca
     1021 tcaccgggct gggtcaaggc gtgaaagact gtcatgttgc cgagcatgct gcccatcagg
     1081 cggtgatccg catagttaca gcggaacgcc accttgagca actcgacgca gagcgcctcc
     1141 acttcatcga tgtgctgagt tcccgcaaac cagcgattga tgcgcccgat gtttccttcg
     1201 gcggcccgtg tgccgatttc agaggacagc aaggcccgca ccattgggct ggtcggtgct
     1261 tcgggtgcca gaagattgag gcagcgctcg ccgcgccatt cttcgtttct ctttactgcg
     1321 tcgatgactg cctgtgccca ttgcctgggc gactcagtcg cgctcagaat ggacctggca
     1381 tttgccagca cgtcccgatc gtgaacttca aattgcgcgg attcgctcat tcaatctcct
     1441 tctgttgtgt tccatcacca ccgtcattta tcatcaaaat cagcaataag gttatccaac
     1501 aaattctgat ttagtcgtgt gcgcttggtc ttttccaact cgatgccaat cacgtttccg
     1561 cgaatttcgt tgttgtagat cgtgtgatta ttgggagtga tgggtgacag ttctgcatcc
     1621 agggctcttg ttccatggtt ctgattggcg gcgatgcgaa ttgctttttt gttttgaaac
     1681 aatttgtttc tttcaatatg ccagtcctgg ctggtctggt tcaccccatg cacctttttt
     1741 agaaaatcgt aaaagcgagt ccagatcaga atgccgtgcg tattattttg gatatcgttc
     1801 tgaatgaccc tgaatcccac gccattttct acagcaactc ccgcctgacg gttgttataa
     1861 atttggtttt cctccagggt gcagtcgctt gaaaacccga gccagaatcc gtagttggaa
     1921 tgattggctt tatttcgctg gtaaatgttg cccttgctga atactccctc aaaagcgttg
     1981 ttgggactat aagaggcgtc gttctcttca aataaattat tgttacatcc gacatcgatc
     2041 ccgtctggag tgagccctgc caggaaaaat ccatctccgc ccaaacgggc attgtttctt
     2101 cgaaaaacat tattgcacga tttatagatg atgagaaacc cggtggcatc cgctcccaga
     2161 tgagagctac tactgcttag ataatatctg cagcaataat cggcataatt ctcagtgaag
     2221 gtactgtccg aagtttcaaa tagatgaaat ccaaagccgc tgcaataatt ggcgaggttt
     2281 ctggctacat cgagtctctg gcattgatag ctgaggagcc cgttcatctg atgctgcaaa
     2341 tcattgtcat agatttttgc atctgtaact tcctggagaa atataccgcc accgtacgat
     2401 tcagccgctg gtttccatat atccagaaat aaagtattcg atgctacgtc tgccgtaccg
     2461 gtgatcgtgc aagtagag
//
LOCUS       DASTJP010000326         6805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_437226, whole genome shotgun sequence.
ACCESSION   DASTJP010000326 DASTJP010000000
VERSION     DASTJP010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437226"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFQ23_09750"
     CDS             complement(<1..407)
                     /locus_tag="VFQ23_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906916.1"
                     /translation="MSTTELSPQGADNKLAKLGAEQAKKARATYTARASSDFYGGIGW
                     MVGLFLTLFISAPVIWFLMNGASSRSAREQILYYGIAIAWWLNPLSLGALGYIFSNIR
                     SWRFWTILALLIWAGINFLVSASIVNETARILT"
     gene            574..1578
                     /locus_tag="VFQ23_09755"
     CDS             574..1578
                     /locus_tag="VFQ23_09755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9906917.1"
                     /translation="MMHDVAVTRLLQKAIDARESLFDARHETAFRLFNGFTEGNPFLS
                     LDLFAGTVIFHNYNPEVSAGMDIVNEARAWLQNHPRFSGWLHAGIVKSRHSKLQEERR
                     GKLLFGQTIDRKINENHVWYAVDLTMNRDMSFYPDTRFLRQWLIENMRGKRVLNTFAY
                     TGSLGVAALAGGAEQVVQLDRSRQFLNIAKDSYMLNAFPIHTNDFMVEDFFPAVSKLK
                     RSGTNFDCVILDPPFFSSTSKGKIDQVNESKRLINKVRPFIENGGYLIAINNALFISG
                     KDYMQTLEELCADGYLKIEQLIPIPQDFVGYNTFDAPIIDPSPFNHSTKIVILKVRRK
                     "
     gene            1596..1973
                     /locus_tag="VFQ23_09760"
     CDS             1596..1973
                     /locus_tag="VFQ23_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9906918.1"
                     /translation="MNNKQILENFWATMETNDFRAASQLLHDDFILDWSQSGERILGR
                     ENFAAININYPAEGKWHFEINHIAADGNLVVTDVSVTDGKISGRAITFSTIRDGKIWK
                     QVEFWPDAFNAPAWRAQWVQNLR"
     gene            2005..2568
                     /locus_tag="VFQ23_09765"
     CDS             2005..2568
                     /locus_tag="VFQ23_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9906919.1"
                     /translation="MEITQTLYVTNAKDWRTWLKKHYKTEKEIWLVYYKKESGKPRIA
                     YNDAVEEALCFGWIDSIVKKLDEERTVQRFSPRKPNAKYSQANIERLRNLAVKKKVIK
                     PVAESIQDILNEKFKIPPDILKAIKSNKEAWKHFQKFSDSYKRIRIAFIDGARKRPHE
                     YKKRLRHFIEMTEKNKMFGFGGIEKHY"
     gene            complement(2581..3459)
                     /locus_tag="VFQ23_09770"
     CDS             complement(2581..3459)
                     /locus_tag="VFQ23_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9906920.1"
                     /translation="MRFHIGCPIWSFKGWVGNFYPEGTKPAEFLHEYSRRLTTVEGNT
                     TFYAVPSQDTLEGWVTQMHETFRFCPKVPKAISHEGKLVDNIERAHEFIRVMSQLGIH
                     LGPMFLQLPPRYSPKLLPDLREFLSAWPREVRLAVEVRHLDWFDSPHDDSLDQLLMEN
                     NMARVTIDTRPIRDLSGDQILAGSAYQSLLEARERKPDVPVVPKRTADFVFVRYIGHP
                     KLERNFPLLDEWADYFVSQLKEGADVYAFCHSPENMTAPWICKELYQRVEKEVGIPAL
                     PWDEIESGKPEQPQLF"
     gene            3660..5465
                     /gene="ftsH"
                     /locus_tag="VFQ23_09775"
     CDS             3660..5465
                     /gene="ftsH"
                     /locus_tag="VFQ23_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9906921.1"
                     /translation="MKQSVNQILDFFKKHKYGKMSLILVLIGLIAFATVLSRSEPQPE
                     PIPLSEVAAAISAGEVLRVEDSQESGITTIYYKDGKKNTTRRDQTAPFLEQMQLLGVS
                     NTQLSRLHYEIVETSQLTGEKTINLLISVAMLGLLGFAMFRLTGGPMNGIRKKYTEGD
                     IPNLSFGDVAGIDESREELVDIVTFLKNGSQYERMGARMPRGVLLVGDPGTGKTLLAK
                     AIAGEAGVPFFTTSGSEFVEVFVGVGASRVRSLFKKARAKAPCIIFIDEIDAAGRKRN
                     ATGGGSEMEQDQTLNQLLVEMDGFAPSDGIVVIGATNRVDVLDPALTRPGRFDRHVNV
                     PRPDVKGRHQILNVHVKNRRLAPDVNLMDIAKSTPGLVGADLASIVNEAAILAVRGGH
                     DVITVQDFQEAVEKNLTGSVQQKSRVMKEDERRTIAYHEAGHAVVMHATPNSDPVYKI
                     TIIPRGQMGGFTMSLPEQDRLLMSKNQILARITGLMGGRVAEEVFCDDITSGASSDLK
                     IATQLAEEMVMRLGMSVTGLRVFNQPEGFDALSSPRTGQKTFEALDEAINHILNECYS
                     EARRIVTEKREAMERVTQHLLQQETLSREEFVALM"
     gene            complement(5539..6477)
                     /locus_tag="VFQ23_09780"
     CDS             complement(5539..6477)
                     /locus_tag="VFQ23_09780"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET9906922.1"
                     /translation="MKTGIVGSGLVGATAAYALVMRGVGRRVVLVDQKRARAESEADD
                     ILHAVPFANPLEVIAGDYADLAGCKVVVVAAGVNQKPGETRLQLLGRNAEVFKQVIPG
                     ILEHAPDAILLVATNPVDVMTHLAARYAAEIGVPSSRVIGSGTTLDTARLRSLIGRKL
                     NIDSQHIHAYVLGEHGDSEVIPWSLVTVGGIPLEKFCTGDEISICPEDYAAIDERVRR
                     AAYHIIEGKGATYYGIGSAIARIVEVILQGERSILTVCTPMEDVAGIKDVTVSLPNLV
                     GGEGIIQTFYPNLNEAETSTLRNSAQVVRSVIDQLK"
     gene            complement(6509..>6805)
                     /locus_tag="VFQ23_09785"
     CDS             complement(6509..>6805)
                     /locus_tag="VFQ23_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9906923.1"
                     /translation="LHIHHFEDEIIHVLDGAIDIRLDSKNLQSEAGGIIHLPKGLPHA
                     IRNPLKTPLKILVFTIPGGLEGYFDEVDAALQNGSLDKNTHNKISQKYGLEWLE"
BASE COUNT         1889 a         1675 c         1632 g         1609 t
ORIGIN      
        1 aaggtcagga tgcgtgctgt ttcattcaca atcgaagcag acactaaaaa gttaataccc
       61 gcccagataa gcaatgccag gattgtccaa aaacgccagg aacgaatgtt gctaaaaata
      121 tatccaagtg cacccaggga aagcgggttt aaccaccagg caatcgcaat cccataatat
      181 aaaatctgct cacgagcaga acgggaggac gcaccattca tcagaaacca aattacagga
      241 gccgagataa aaagtgtaag aaatagcccc accatccagc caatcccgcc ataaaaatca
      301 ctggaggctc tggctgtata agttgctcgt gccttttttg cctgctctgc acccaatttg
      361 gccagcttgt tatctgcgcc ttgaggggat agttcagtag tggacataag ttacctcata
      421 ataactgcgt tgatgcgttt gtgagctagt ctaccctaaa aaaggtaggg gtacaatgca
      481 aggttgaaac tctcattcgc tcttaattca gtacgcataa ataggacgtt tgaatcttca
      541 aagtcttaca tttttgaatt agactgtaat cctatgatgc acgacgtggc agttacacga
      601 ttgttacaaa aagcaattga cgcacgcgag tcgttgttcg acgcacggca tgagactgcc
      661 tttcgcctgt tcaacggatt tacggaggga aacccatttc tttctttgga cctgtttgct
      721 ggcaccgtta tttttcacaa ctacaatcct gaagtatccg ctggtatgga tattgtcaat
      781 gaggcgcgag cctggcttca aaaccaccct agattttcag ggtggctaca cgctggaata
      841 gtaaagtcgc gccatagcaa gttgcaggag gaacgacgcg gcaagttgtt attcgggcaa
      901 accatagatc gcaaaatcaa tgaaaatcac gtctggtatg cggttgatct gaccatgaac
      961 cgcgatatga gcttctaccc tgatactcgc tttttgaggc agtggctaat tgaaaacatg
     1021 agaggcaaac gtgtattgaa cacgttcgct tacacgggca gcctgggcgt ggcggcgctg
     1081 gcaggcggag ctgaacaagt cgttcagctt gaccgcagtc gccagtttct gaacatagca
     1141 aaagactcat atatgctgaa tgcgtttcca atccatacca atgattttat ggtcgaagat
     1201 ttcttcccgg ctgtcagtaa actaaaacgt tcaggcacaa acttcgattg cgtaatcctt
     1261 gatcctccct tcttctccag cacctccaaa ggcaaaatag atcaggtgaa cgagagcaag
     1321 cgcctcatca acaaggtaag gccgttcatc gaaaacggcg gctatttgat cgcgattaat
     1381 aacgccctat ttatcagtgg aaaggactat atgcaaacgc tcgaagaact ctgtgccgac
     1441 gggtatctca aaattgagca gttgattcct atccctcagg atttcgttgg ctataataca
     1501 ttcgatgctc caatcatcga cccatccccc ttcaatcact ccacaaagat cgttatattg
     1561 aaggtacgga gaaaatgaca gatcatggag aatggatgaa caacaagcaa attctggaaa
     1621 acttctgggc aacgatggag accaacgatt tccgcgctgc ctcccaactc ttacatgatg
     1681 actttattct ggattggtcc cagtccggcg agcgcattct tggcagagaa aattttgccg
     1741 cgatcaacat aaattatcca gctgaaggca aatggcattt tgagatcaac cacatcgcgg
     1801 cggatggaaa cctagtggtc acagacgtga gcgtcaccga cggaaaaatt tctggtcgcg
     1861 cgattacatt ctcgacgatc cgggatggga agatatggaa acaagttgaa ttttggcccg
     1921 atgcctttaa tgctcccgcc tggagggcgc aatgggtaca aaacctaaga tgatcgtata
     1981 aaaaaatagc accaggtcaa aaccatggaa atcacccaaa ccttatatgt aacgaacgcc
     2041 aaagactggc gaacgtggct caaaaagcac tacaagaccg aaaaagagat ctggctggtc
     2101 tactacaaaa aggaatcagg gaaaccacgc attgcctaca atgacgcggt ggaggaagcc
     2161 ttgtgcttcg gctggatcga cagcatcgtc aagaagctgg atgaggaacg caccgtccag
     2221 cggttttcgc cgcgaaaacc aaatgccaaa tattcccagg ccaacattga acgactgcgg
     2281 aacctggcgg taaagaaaaa ggtaatcaaa ccggtcgcgg aatcaattca agatattttg
     2341 aacgaaaaat tcaaaatccc gcctgatatt ttgaaagcca tcaagtcaaa caaagaggca
     2401 tggaaacatt ttcaaaagtt ctcggattca tataaacgaa tacgaattgc ctttattgat
     2461 ggggcaagaa agcgaccaca tgaatacaaa aagcgtctcc gccattttat cgagatgact
     2521 gagaaaaata aaatgttcgg atttggtgga attgaaaagc attattaacg taagacagtt
     2581 ctaaaataac tgtggctgct ctggtttgcc cgattcaatc tcgtcccagg gtaacgctgg
     2641 gattccaacc tctttttcca cacgttgata tagctcttta cagatccagg gcgcggtcat
     2701 gttttcaggc gaatgacaga atgcataaac gtccgcaccc tctttaagct gtgacacaaa
     2761 ataatctgcc cattcatcca gaagcggaaa attgcgttcc agcttcgggt gaccgatgta
     2821 tcggacaaat acaaaatccg cagttcgctt tggaacaacc ggaacatccg gctttctttc
     2881 ccgcgcctca agcaaactct gataagccga acccgccagg atttgatccc cacttagatc
     2941 gcgaattggg cgtgtgtcta tcgttacacg cgccatattg ttttccataa gcaactgatc
     3001 gagcgagtcg tcatgcgggg agtcaaacca gtccaaatgg cggacttcaa ctgccaggcg
     3061 cacctcacgc ggccaagcgg agagaaattc ccgaaggtca gggagcagct tcggggaata
     3121 acgcggcggc agttgcagga acatcggtcc gaggtgtatt cccaactgac tcatcaccct
     3181 gataaattca tgcgcccgtt cgatgttatc caccaacttg ccttcatggc tgatcgcttt
     3241 tggcactttt gggcagaaac gaaatgtctc gtgcatctgt gttacccagc cttcgagcgt
     3301 atcctgtgaa ggcacggcat aaaaagtggt atttccttct acagtggtga gccgacgtga
     3361 atattcgtgc aggaattcag ccggtttcgt gccttccggg taaaaattac cgacccagcc
     3421 tttgaacgac cagattgggc aaccaatatg aaaacgcata cttttattat agtacggctt
     3481 gatgaggtgg acacgatacg gcttcaatac tcagaaaaat ttaagttaca aaaccaaaac
     3541 atcggcgtca tggattaata atctgttaag catgtctaat gaaattgaaa gctcaaaagc
     3601 aggcttaacg aggtaacatg tggaaacttt gatatgtccg tgaacggata gaggtagtaa
     3661 tgaaacaatc agttaaccaa atcttagatt tcttcaaaaa acataagtac ggaaagatga
     3721 gcctgattct cgtgttgatt gggctcattg catttgctac agtgctttca aggtcagagc
     3781 ctcagcccga gcctattcca ctgagtgaag tggcagcagc catctctgcc ggcgaggtgc
     3841 tcagagtgga ggactcccag gaaagtggca tcacgaccat ctattacaaa gatggaaaga
     3901 aaaatacaac ccggcgagat caaacggctc ccttcctgga gcagatgcag ttgctgggcg
     3961 tgagcaacac gcagctttca aggctccact acgagattgt ggaaacaagc caactcactg
     4021 gtgagaaaac catcaaccta ttaatcagcg tggcaatgct gggtttgctg ggcttcgcca
     4081 tgttccgcct gaccggtgga cccatgaacg gcattcggaa gaaatacacc gagggagata
     4141 ttccaaatct tagctttggc gacgtggccg ggattgacga aagccgtgaa gagcttgtgg
     4201 atatcgtcac tttcctgaaa aacggatcac agtatgaacg gatgggagcc cgtatgcctc
     4261 gaggggtctt attggttgga gacccgggaa ctggcaagac cttgctagcc aaggcaattg
     4321 ccggagaagc tggtgtgccg ttctttacta cctccggttc cgaatttgtc gaagtctttg
     4381 taggcgttgg cgcgagtcgt gtccgctctc tctttaagaa agcacgcgcg aaagctccat
     4441 gtattatttt tatcgatgag atcgacgccg ccggacggaa acgcaatgcc accggtggtg
     4501 gcagtgaaat ggaacaggac cagactctca atcagttact ggttgagatg gacggatttg
     4561 cacccagtga tggaattgta gtcattggcg cgaccaaccg cgtggatgtg ctcgacccgg
     4621 ctcttacccg acccggtcgc ttcgaccgcc atgtgaacgt gcctcgaccc gatgtcaaag
     4681 gacgacatca aatactaaac gtacatgtga aaaatcgccg ccttgcgcca gatgttaatt
     4741 tgatggacat tgccaaatcc acacccggac ttgttggcgc agatctggcc agcatcgtca
     4801 acgaagcagc cattctggcg gtgcgtggcg gccatgatgt catcactgtg caggacttcc
     4861 aggaagccgt agagaaaaac ctcaccggca gcgttcagca gaagagccgc gtaatgaagg
     4921 aagatgagcg acgcacgatt gcctatcatg aagctggtca tgcggtagtg atgcatgcca
     4981 cgccaaattc tgacccggtc tataaaatta ccatcatccc cagaggtcag atgggtggct
     5041 ttacgatgtc acttccagag caggacagat tgctcatgtc aaagaaccag atccttgcgc
     5101 gaattactgg tctgatgggt ggccgcgtcg ctgaagaagt attctgcgac gatattacca
     5161 gcggcgcatc cagcgacttg aagatcgcta cgcaactggc ggaagaaatg gtcatgaggc
     5221 tcggaatgag cgttacgggc ttgagagtat tcaaccagcc cgaaggattt gatgctctct
     5281 cttcaccgcg cacgggtcag aaaacattcg aagcgctgga tgaagcgatc aatcacatcc
     5341 tgaacgaatg ttatagcgaa gccaggcgaa tcgtgaccga gaaacgtgaa gcgatggaac
     5401 gagtcacgca gcatcttttg cagcaggaaa cgctttcacg tgaagaattc gtagcattaa
     5461 tgtaatacaa aacactctac ataaaacaaa agtcggctgt gcagccgact tttgttttat
     5521 aaactattct ttcagccttc attttaactg atcgatcaca gatctcacaa cctgagcact
     5581 gtttcgtaat gtggacgtct ctgcctcgtt gaggttggga tagaacgtct ggatgatccc
     5641 ctctcctccc accaagtttg gaagtgaaac ggtgacatcc ttaatcccgg ccacatcctc
     5701 catgggtgtg cagaccgtga gaatggaacg ctcgccttgc aggatcacct caacaattcg
     5761 ggcaatcgcg ctgccgatgc cataataggt tgcgcctttt ccttcaatga tatggtaagc
     5821 cgcccggcga actctctcat cgatcgccgc ataatcttca gggcagatgg atatctcatc
     5881 ccctgtacaa aatttttcca gaggaattcc gcctacggta acgagggacc agggtatcac
     5941 ctcagagtcg ccgtgctcgc caagcacata ggcatggatg tgttgagagt cgatattcaa
     6001 tttcctgccg atcaagcttc gcaaccgcgc cgtatctagc gtagtccctg acccgattac
     6061 acggctggag ggaactccga tttctgccgc gtatcgagcg gcgaggtgcg tcatcacatc
     6121 gaccgggttg gtcgcgacca acagaatcgc atctggagcg tgttcgagaa tcccagggat
     6181 cacctgcttg aatacctcgg cattacgccc aaggagttgc agacgtgttt caccgggctt
     6241 ttgattaacc cctgccgcga cgactaccac tttgcaacct gcaagatctg cgtaatctcc
     6301 tgcgataacc tccagcggat tcgcaaacgg aaccgcatgc aaaatgtcgt ctgcttcaga
     6361 ctcagcgcga gcccgttttt gatccactaa aaccacgcgg cgccccaccc cacgcatgac
     6421 cagggcgtag gcggcagtcg cgccgaccaa tccactccca acaattcctg ttttcattga
     6481 cgtttctctt tcgaaagatt attgatcatt attccagcca ttcaagcccg tacttttgcg
     6541 agatcttgtt gtgcgtattc ttatccagcg agccattttg taaggcagca tccacctcat
     6601 caaagtagcc ttccagccca ccggggatcg taaataccaa tattttcaac ggagttttta
     6661 atggattgcg aattgcatgt ggtagacctt taggcaggtg aatgatccca ccagcctcgg
     6721 attgtaagtt cttgctgtcc agccgaatat caatggcacc atccaaaaca tggattatct
     6781 catcttcaaa atgatggatg tgtaa
//
LOCUS       DASTJP010000327         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_147736, whole genome shotgun sequence.
ACCESSION   DASTJP010000327 DASTJP010000000
VERSION     DASTJP010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147736"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VFQ23_09790"
     CDS             <1..1409
                     /locus_tag="VFQ23_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9906924.1"
                     /translation="GRNSQFTDPSYHMPHYYKLWALWADKNNDFWSEAAQVSREYLPK
                     SVHPQTGLAPNYSEFDGSPVSDPYNGNFRYDAFRVGANVGMDYVWFAPGEWHVEQSNR
                     LLSFFASQGMDEYKAEYLLSGEPQVGHRSSGLMATNAVAALAADREIGEPFVQALWDL
                     DVPTGQFRYYDGLLMILGLLQVSGNFRIYEPGTAPLGQVFPTPKPEVNGKFAPPTGRA
                     LLLVGQDAKSIDSYFDATVTAPGGIAVNTSLQLEGLEDLNYLAGKYLKSALSVGVDLN
                     DSIHDVAAGNADANIDAMLDALNAHNRPVFIRLGYGFDDPAKAYASDVYVSAWKRFYE
                     RIQAKGSQNFALVWESTSCEETKIADWYPGDEFVDWIGLPYCDGMSTASVQFAREHFK
                     PVMMIAAPSSPIGGWNEWFAPFFLFVNENNDVVRSIAYLNTDVLPADTLKLWKEETKQ
                     TFWLRASPSLFDTLGYTE"
     gene            1432..>2367
                     /locus_tag="VFQ23_09795"
     CDS             1432..>2367
                     /locus_tag="VFQ23_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9906925.1"
                     /translation="MSVMDTSEKLLYLDTSLSIAERAKDLVGRLTLEEKVGLMSHPAH
                     GVPRLNIPAYNYWSEALHGVARNGRATVFPQAIGMAATWDKDLIYQVASAISTEGRAK
                     YHEALRRNGYTAQYQGLTFWSPNVNIFRDPRWGRGQETWGEDPFFTGEMASAFVRGLQ
                     GDHPVYLKAAACAKHYAVHSGPEKDRHTFNAIVTKRELYDTYLPAFKKLVMDAKIEAV
                     MGAYNRTLDEVCCASKLLIEDILREEWNFQGHFVSDCGALSDFHLNHKVTKDAAESAA
                     LALEHGCDLGCDHVFDEIPEAIRRGLITEADVDRSL"
BASE COUNT          577 a          575 c          623 g          592 t
ORIGIN      
        1 ttggccgcaa ctcacaattt accgacccgt cgtatcacat gcctcactac tacaaactct
       61 gggcactgtg ggcagacaag aacaatgact tttggtccga agccgcgcag gtcagccgag
      121 aataccttcc caaatctgtc catccacaaa ccggacttgc ccccaactac tctgaattcg
      181 atggcagccc agtgagcgac ccctacaacg gcaactttcg ctatgacgcc tttcgagtgg
      241 gcgcaaatgt gggcatggat tacgtttggt tcgcgccagg tgagtggcac gtggaacaat
      301 ccaatcgttt gttaagtttc tttgcctctc aaggcatgga tgaatacaaa gccgaatatt
      361 tattgagcgg tgaaccacag gtaggtcatc gctcatcggg tttgatggca acaaacgccg
      421 tcgctgctct cgcggcggat cgtgagatcg gtgaaccgtt cgtccaggct ttgtgggatt
      481 tggacgtccc gacaggtcaa ttccgttatt acgatggctt gctcatgatt ttggggttac
      541 tgcaagtcag cggcaacttc cgcatctatg agccaggcac cgcacctttg ggtcaggttt
      601 tccccacacc caagcctgag gtaaatggaa aatttgctcc cccaacggga cgagcattat
      661 tgctcgtcgg gcaggatgcg aaaagcatag actcctactt cgacgcgacc gtcactgcgc
      721 caggaggaat cgctgtaaac acatccctgc aattggaagg tctcgaggat ttgaattatc
      781 tcgcagggaa atatcttaaa agcgcgttat cggttggggt ggatttgaac gattcgatcc
      841 acgatgttgc tgcaggaaac gcggatgcaa atatagatgc gatgctcgat gcgttgaatg
      901 cacacaaccg tcccgttttt attcgtttgg gttatggttt tgatgatcca gccaaggcgt
      961 atgcgtcaga cgtgtatgtg tcagcgtgga aacgattcta tgaacgcatt caggccaaag
     1021 gtagtcagaa cttcgctctt gtgtgggaat ctacttcctg tgaagaaacg aaaatcgcag
     1081 attggtatcc aggtgatgag tttgtcgatt ggattggact gccgtattgc gatggaatgt
     1141 ctaccgcatc agttcaattt gcgcgtgaac acttcaaacc tgtgatgatg atcgcagcac
     1201 cttcaagtcc tatcggaggt tggaatgaat ggttcgcgcc gttcttcctg tttgtgaacg
     1261 aaaataatga tgtcgtgcgg tctatcgcgt atctaaatac agacgttttg cctgctgaca
     1321 ctttgaaatt gtggaaagaa gagaccaaac agactttctg gttgcgtgcc agcccatctt
     1381 tatttgacac attaggatat acagaatgaa cttatctcaa aggagcgctt catgagtgtg
     1441 atggatacat cggaaaaact tttgtatctt gatacatcgc tttccatcgc cgaacgggca
     1501 aaggatttgg tcgggcggct cacgctggaa gaaaaagttg gcttgatgag ccatccggcc
     1561 catggtgtgc cgcgcctgaa catccccgct tacaactact ggagtgaggc attgcatggc
     1621 gttgcgcgca atggacgcgc cacggtgttc ccgcaggcaa ttggaatggc agccacctgg
     1681 gataaagact tgatttatca ggtggcttct gccatcagca cggaaggacg cgccaagtat
     1741 catgaagcct tacgccgaaa tggatataca gctcaatatc aggggctgac cttttggtca
     1801 cccaatgtga atattttccg cgacccacgt tggggacgcg ggcaggaaac ctggggcgaa
     1861 gaccccttct ttactggtga aatggcatcc gcttttgtga gaggcttaca gggcgatcac
     1921 cctgtttatc tcaaggcagc tgcctgtgca aaacactatg cggtgcattc gggtcccgag
     1981 aaagatcggc acactttcaa cgccatcgtg accaaacgcg aactgtatga cacctatctg
     2041 cctgccttca aaaaacttgt tatggatgca aagatcgaag cagtcatggg cgcctacaat
     2101 cgcacgctcg atgaagtctg ttgcgccagt aaacttctga tcgaagatat cctgcgcgag
     2161 gaatggaatt ttcagggaca ttttgtttca gactgcggag cactgtcaga ttttcatctc
     2221 aatcataagg tcacgaagga cgcggctgaa tccgctgcac tggctttgga acatggatgc
     2281 gacctggggt gtgatcacgt ttttgatgag attcccgagg cgatccggcg tggccttatc
     2341 accgaggcag acgtcgatcg ctcactg
//
LOCUS       DASTJP010000328         9420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1512104, whole genome shotgun sequence.
ACCESSION   DASTJP010000328 DASTJP010000000
VERSION     DASTJP010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512104"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFQ23_09800"
     CDS             <1..468
                     /locus_tag="VFQ23_09800"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906926.1"
                     /translation="SGANQEPNVVFVAPNETLSSYELIQKSKFVMVYNSTIGLEASIM
                     GAPVLCAGKARFTQYPTVFFPQMVEEIRRKMEEFLEADKIDIPPEFKSNARRFLYYQL
                     FRTSLPFGDFLEPSVRTTQTKLKSFTVDDLLEADAVKVILNGVLRGGDFLLEE"
     gene            476..1273
                     /locus_tag="VFQ23_09805"
     CDS             476..1273
                     /locus_tag="VFQ23_09805"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9906927.1"
                     /translation="MREISLEKPIAHGRTADVYDWDEAYILKLFHNWFELPNIEYEFK
                     IASAVHASGERAPAVRELIQIQGRNGLVYERVAGKSMLEVLLQKPWMAFKYARVLASL
                     HAQMHERVFDGVGIPAQRKRLENKINQASALSASLKGSLLNALQSLPEGDRVCHGDFH
                     PANVLVAGEVANVIDWMDASQGNPLADVARTSIIALGEAESRETLNPLMKSFIKLFHS
                     IYLNHYFRLRPGGEAEYRRWLRIVAGARLSEGITELEKWLVKEAEKI"
     gene            1343..1855
                     /locus_tag="VFQ23_09810"
     CDS             1343..1855
                     /locus_tag="VFQ23_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906928.1"
                     /translation="MEKAKNLFFNYSCSRFFMSRDGADYEYDQYGVTAEQEAIWRKEY
                     IDFWTSRLSVDDLEALNNLDHAWAIEAIPDLIKICGQAEGYAKLQYADSIWQLSKSSK
                     LQGDIRKQAIETSIAAWESLLTGNFNIPEQFRKKIISLMPRIEATTAENYVINSAKRQ
                     LEEAKKRGNQ"
     gene            1984..2445
                     /locus_tag="VFQ23_09815"
     CDS             1984..2445
                     /locus_tag="VFQ23_09815"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9906929.1"
                     /translation="MQTFFKNYLELLQDCHNGILQAIEGLPPAAMDWVPGKDMNSIAV
                     LLAHTIGSVRYWIGDIAAQESSNRDRAAEFRVRGVEADVFKTKLADNLEYARNVLDKM
                     NLQDLESTRISGDGRELTVAWALLHALEHATSHMGQIQITRQLWEQSKGNA"
     gene            2533..2694
                     /locus_tag="VFQ23_09820"
     CDS             2533..2694
                     /locus_tag="VFQ23_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906930.1"
                     /translation="MAKVNYQYEKRQKELEKKRKKEQKEKLKQIKKNIQPTETQETPQ
                     TPQVPPPEK"
     gene            complement(2850..4898)
                     /locus_tag="VFQ23_09825"
     CDS             complement(2850..4898)
                     /locus_tag="VFQ23_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906931.1"
                     /translation="MWHGGGWFMYMRQGDEKPKVTKQLLLRVFSYARGYWWHIAGMLV
                     TISLTTGLSLLTPLIFRNMIDVVIPAKDVNRLILLGVALLLIPAVSGGINVIQRRLNS
                     TVGEGVIYDLRSALFARLQRMSLRFFTNTKTGELMSRLNGDVVGAQNAISNTIVNIVT
                     NFIEAIALIAVMLTLEWRLTVVSVLILPLFIIAARRLGTVLRDIAREAMETNAQMNAH
                     MNETLNIGGALLVKLFGRSRDEENRFRERAANVRDIGIRRAVVGSTFFVIFGLVSAVG
                     TALVYGLGGYFVITDVFTVGTIVAFGSYLGNLYGTLQGLAGAPVEFSTSMVSFERVFE
                     LIDLPQDIVEKENALILQDVRGELEFDNVTFNYHMDESKLLKDVKRYGRHEDVGAVLS
                     LAKPATDNGPKTKDAGKQNMEGKNGQSSTPALASGASVVYGQENGRTESDSGEADYKS
                     QAREVALEGVSFNARPGQLIALVGPSGAGKTTMTYLIPRLYDPTEGVIRIDGHDLRDV
                     TLESLSLAIGMVTQETYLFHDTIRTNLTYAKLDATQAEIESAARAANIHNFIMDLPDG
                     YDTIVGERGYRLSGGEKQRIALARVILKNPKILVLDEATSHLDSESESLIQEALKRVM
                     AGRTSIVIAHRLSTILAADLILVMDRGKIVERGTHNELIALNGLYAQLYETQFNRERV
                     "
     gene            complement(5065..5781)
                     /locus_tag="VFQ23_09830"
     CDS             complement(5065..5781)
                     /locus_tag="VFQ23_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906932.1"
                     /translation="MKAYNCPNCGAALPARALKSDLVECEFCGTTFRISKTLTPEPEM
                     GDLMLGADFKDDVIPGWEVFNEPKRLTFHRGDPSELHGIYEPSYNSYYVLKSSGFLDD
                     FDASINMKFTEGDKNIIRAGFYLRFTPEGGGYAVLVSTLGSYTIGYYVKGEKGELVWE
                     DLIAWSNHTALRPGMHETNRLRVICDREKFRVYLNGTLATSFKDDRFKRGRLYVAVVP
                     TEKSSLGIVFSDLQLREVLK"
     gene            5961..6197
                     /locus_tag="VFQ23_09835"
     CDS             5961..6197
                     /locus_tag="VFQ23_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906933.1"
                     /translation="MKHWDTASVSDAGDHFIPAAAAFGGSSAFAWMSQMLQTKWFSEV
                     SHKLVTIERNWIWIIAALVFAIFHIFVLGPGVEL"
     gene            6199..6534
                     /locus_tag="VFQ23_09840"
     CDS             6199..6534
                     /locus_tag="VFQ23_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906934.1"
                     /translation="MTNRTPAIISAVITILLLVLLAGLSVFMQMIALNGASERQGTTA
                     MSISLICQGIGLILAGLFAGWLSNFVINRFNWNKILAVIIAVILGVLIGGVISVVSFF
                     ISLPLAGIV"
     gene            6620..7378
                     /locus_tag="VFQ23_09845"
     CDS             6620..7378
                     /locus_tag="VFQ23_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HET9906935.1"
                     /translation="MKALTVLFVLLLLFYACVPQRTEPAPPHWTYEGEEGPSHWGELD
                     PGYAVCGTGQSQSPIDVVNASEQDLSNIAFHYQPSEVNILNNGHTVQVNYDTGSYIEV
                     DGTRYDVVQFHYHAPSEHTLDGQSFPAELHLVHRNADGNLAVVGILLKEGMENAAYQP
                     FINHLPPEKADAQDAGVPVNAGDLLPSVETTFRYSGSLTTPPCTEGVSWLLMTTPVEL
                     SATQLGALDSLFEGGNNRPTQPLNDRSLLQDTTP"
     gene            7459..8178
                     /locus_tag="VFQ23_09850"
     CDS             7459..8178
                     /locus_tag="VFQ23_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9906936.1"
                     /translation="MTIETASSSEITDHFIPELFFDYLFISEGTIKAVEPSRETDVSL
                     PRECLKTIHTRPTTFLYSTPCVLFGARFSLRFAELFWEKELPSNSFLKQNWMEINTND
                     LESFGDQVIRYVQEHQDRKTAYPMLKHNLDESDWLVHFSSRHKRRLYKTVFGVSKKDL
                     QNIQNIHSFLEQTCDFSSENPRIIQYINADVFHDQPHLNHAFRNMTGFSPVEYFEANS
                     ILQDNLMSASYNEFPSGVDIL"
     gene            8182..8541
                     /locus_tag="VFQ23_09855"
     CDS             8182..8541
                     /locus_tag="VFQ23_09855"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9906937.1"
                     /translation="MKYTKSKNDIVNEYIDALDTAIAPLFLEIRDCILKAGPAFNESI
                     KWKNCLVYGTTKNHIQTVVGKGKISLIFFEGVSLQDKYGLLEGEGNKARTMRISSPDF
                     NKTALGDYVKQTLKNET"
     gene            8553..8969
                     /locus_tag="VFQ23_09860"
     CDS             8553..8969
                     /locus_tag="VFQ23_09860"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9906938.1"
                     /translation="MRKYSASTTINASPEKIWSILTDASSYPTWDTSMDRIDGKLALG
                     ETVKFFTKLSSQAFPVKVTGFEPNRQLTLTGGMPLGLFKSERTHRLTSNKDGTTTFQT
                     EEVFSGLLLPIFGRTIPDLTENFKAFAAGLKKQSEK"
     gene            complement(9021..9377)
                     /locus_tag="VFQ23_09865"
     CDS             complement(9021..9377)
                     /locus_tag="VFQ23_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906939.1"
                     /translation="MKKTSTTLLVFGIYLIGMGLGFLFTPNILLNLFGIPATDEVWPH
                     VVGAIALVLAFYYMSAARADLRAFAQWTIPARIGIFISFTAFVIAGLVGPIMILLGSV
                     DLLGALWTRSALKAEG"
BASE COUNT         2511 a         2276 c         2306 g         2327 t
ORIGIN      
        1 agcggtgcga atcaagagcc gaatgtagtc tttgtcgcgc caaatgaaac attgagttcg
       61 tacgagttaa ttcagaagtc aaagttcgtg atggtctata actcaacgat cgggttggag
      121 gcttcgatta tgggcgcgcc tgtgctgtgc gcgggcaaag cccgctttac gcagtatccc
      181 accgtgttct ttcctcagat ggttgaagaa ataagaagaa agatggaaga gtttcttgag
      241 gcggataaga tcgatatccc acctgagttc aaaagtaacg caagaagatt tttatattat
      301 cagttgttta gaacttcatt gccatttggg gattttcttg agccctctgt tcgcacaaca
      361 caaacaaagt tgaaatcatt tacagtggat gatttgttag aggcagacgc tgtgaaggta
      421 attttgaatg gcgtgctacg tggtggagat tttttgctag aagagtgaat tacctatgcg
      481 ggaaatttca cttgaaaagc ctatcgctca cgggcgtact gcagatgtct atgattggga
      541 cgaggcgtac atcctcaaac tctttcacaa ctggtttgaa ctcccaaata tcgaatatga
      601 attcaaaatt gcgagcgcgg tccacgccag cggtgagagg gctccggctg tcagggagct
      661 aatccagatt cagggacgaa atgggttggt ctatgagcgc gtggctggca aatccatgct
      721 cgaagtgctc ctgcagaaac catggatggc attcaaatac gctcgagtcc ttgccagttt
      781 acatgcacag atgcatgagc gtgtttttga tggtgttgga atccctgccc aacgcaagag
      841 gctggaaaat aaaatcaatc aggcaagtgc gctttctgcc tcgctaaagg gatcactgct
      901 gaacgcgctt caatccctac ccgagggcga ccgtgtttgt cacggtgatt ttcatccggc
      961 gaatgtgctg gtagctggag aagtggcgaa tgtgattgac tggatggacg catcccaggg
     1021 caatccgctt gccgatgtgg cacgcacctc gattattgca ctgggtgaag cagaaagcag
     1081 ggagacgctt aaccccctca tgaaaagttt tataaagcta tttcattcga tctatctcaa
     1141 tcattatttt cgcctgagac caggcggcga ggctgaatac cgacgctggt tgcgaattgt
     1201 ggcaggcgcg aggttgagtg agggaatcac tgaattagag aagtggctgg tgaaagaggc
     1261 agagaagatt taatagacta tctcgcccag aactttgggg cattttacca agtgctataa
     1321 tccgcttact atgaacgaga acttggaaaa ggcaaaaaat ttattcttca actacagttg
     1381 cagtagattt ttcatgtcgc gtgatggggc tgattatgaa tatgatcaat atggagtcac
     1441 tgcggagcag gaagccatat ggcgaaagga atatattgat ttttggacat cccgcttatc
     1501 agtggatgat ttagaggcac ttaataattt agatcacgcc tgggccatag aggcaattcc
     1561 tgatctcata aaaatatgcg gtcaagctga aggatatgca aaattgcaat acgcagattc
     1621 catttggcaa ctttcgaaaa gttcaaaatt gcaaggggat attcgcaaac aagcgatcga
     1681 aacttcgatt gcagcctggg aatctctttt gacaggaaat tttaatatcc ctgaacagtt
     1741 tcggaagaag ataatttcac ttatgccgcg gatcgaggca accaccgcgg aaaattatgt
     1801 aattaactcg gctaaacgac aattggaaga agcgaaaaaa agaggcaacc agtaggctgc
     1861 gcctacgcgc tttctttggt gactctaagc gagcggtgga ggtggagttt tgacctgaat
     1921 gctataggga ataaacagcc tcacggcggt tgtcataaat ctcgcgaaat gagatggtga
     1981 atcatgcaaa cattcttcaa aaactacttg gaactcctgc aagattgtca taacggtatt
     2041 ctgcaagcta ttgaaggact tcctccagct gccatggatt gggttccagg gaaagacatg
     2101 aactcaattg ctgttttgct ggctcatacg attggttcag tcagatattg gattggcgat
     2161 atcgccgcgc aagaatcatc caatcgcgac cgggctgcgg agtttcgagt ccgcggcgta
     2221 gaggcggatg tttttaagac gaaacttgca gataatttgg aatacgcccg gaatgtgctg
     2281 gataaaatga acttgcagga tttggaatca acgcgcattt caggtgatgg acgtgaactc
     2341 actgtggcat gggcattgct ccatgcactt gaacatgcca caagtcacat ggggcagatc
     2401 cagatcactc gtcagttgtg ggagcaatca aaaggcaatg cttagtttct gctttctcag
     2461 gttccgccca ctgcgagatg ccataatttt ttaaaaggct cactgaatcc aactataaga
     2521 aaggatacca aattggctaa ggtaaattat caatacgaga aacgtcaaaa agaattggaa
     2581 aagaaacgta agaaagagca gaaagaaaag ctcaagcaga tcaaaaagaa tatccagcct
     2641 acagagactc aagaaacccc gcagacaccc caggttccgc caccagagaa gtaggaagtt
     2701 atttgccgtt gcaaaaagag atcagtgcct tcgaggcctg gtctctttgt tttctgatcc
     2761 ccatcctccc tacggggtgc tgctgcgcaa aggtcaaggg cataaatgat aagtcgttga
     2821 acttgataag ccggtagagc ccgttttgat tacactcttt ccctgttgaa ctgcgtctca
     2881 tacaactgag catacaaacc attcagcgca attaattcat tatgggtgcc gcgctcaacg
     2941 atttttcctc gatccattac taaaattaaa tcagccgcaa ggattgtaga tagacgatga
     3001 gcaattacga tactcgttcg tcctgccatg acgcgcttca gtgcttcctg gatcagtgat
     3061 tctgattcgc tgtcgagatg gctggtggct tcgtcgagga caaggatctt cgggttttta
     3121 aggattactc ttgccaacgc gatgcgttgt ttctcgccgc cgctcaaacg gtaaccgcgc
     3181 tcgcccacaa tcgtgtcgta tccatcgggc aggtccatga tgaagttgtg gatgttagca
     3241 gcccgcgcgg cgctttcgat ctctgcctgg gtcgcgtcca gctttgcata agtcaggttg
     3301 gtgcggatgg tgtcgtggaa gagataggtc tcctgcgtca ccataccgat ggctaacgaa
     3361 agcgactcga gcgtgacgtc gcgcaggtca tgcccatcga tgcggatgac accctccgtg
     3421 gggtcataca gtcgcggaat aaggtacgtc attgtcgttt tgccagcgcc ggaaggaccg
     3481 accaaggcga tcaactgccc gggacgtgcg ttaaaggaga ctccctctaa agcgacctca
     3541 cgtgcctggg atttgtaatc ggcttcccct gagtccgatt cggttcttcc attttcctga
     3601 ccatagacca cacttgcacc tgatgcaagt gcaggtgtag acgattgacc attctttccc
     3661 tccatgttct gtttcccagc gtctttggtc tttggtccat tgtctgtggc gggctttgcc
     3721 aacgataaga ccgcgcccac atcttcatgt cttccataac gtttgacgtc tttgagcagt
     3781 tttgattcgt ccatgtgata gttgaacgta acgttatcga attcgagttc gcctcggacg
     3841 tcctgcagga tgagcgcatt ctctttttca acaatatcct ggggcagatc aatgagttcg
     3901 aacacgcgct caaagctgac catcgaagtg gagaactcca caggtgcgcc tgccagcccc
     3961 tggagggtgc catacaggtt gccgagatag gaaccgaacg ctacgatggt tccaacagta
     4021 aagacatccg tgatgacaaa gtaaccgccg agaccataca ccagcgctgt gcctaccgcg
     4081 ctgacaagcc cgaagatgac gaagaaggtc gagcccacca cggcacggcg gataccgata
     4141 tcgcgcacgt tcgcagctcg ttcacggaag cgattctcct cgtcgcgcga acgaccaaag
     4201 agtttgacga gcagtgcgcc gccaatgtta agcgtctcgt tcatgtgcgc gttcatctgc
     4261 gcatttgttt ccatcgcctc tcgcgcgata tcgcgcagaa ctgtgcccag ccgtcgagca
     4321 gcaatgatga agagcggcag gatcagcacg ctgaccacgg tcagacgcca ttcgagcgtg
     4381 agcatgaccg caatcagggc aatagcttcg atgaagttgg tcacgatatt gacgatggta
     4441 ttgctgatgg cgttctgtgc accgaccaca tccccattaa gacgggacat taactcgcct
     4501 gttttggtgt tggtgaaaaa tcgcagggac atgcgttgca ggcgtgcaaa gagcgctgag
     4561 cgtaaatcgt agatcacacc ctcaccaact gtcgagttca gtctgcgttg aatgacattg
     4621 attccaccgc tcaccgcggg gataagaagc agtgccacgc ccaaaaggat cagtcgattc
     4681 acatccttgg cgggaatcac cacgtcgatc atgttgcgga agatcagagg tgtcagcagg
     4741 gagagcccgg tcgtaagaga aatcgtcacg agcatacccg caatgtgcca ccaatagccg
     4801 cgcgcatagg aaaaaactcg tagcaagagt tgttttgtta ctttgggctt ttcatccccc
     4861 tgacgcatgt acataaacca accaccaccg tgccacataa tttatatagt ctcctagttt
     4921 actagtccgc tagtctactc gtcgttccaa cgagagggtt tgaccagccg actagctgac
     4981 tataagacga gatgactaat tttgcaactg gacgaagggg gaggttaaga gtagggtgat
     5041 gagaaaggaa gtcgtaagtt agggttactt tagcacttct ctcaactgaa gatctgaaaa
     5101 gacaatcccc aaacttgatt tctctgttgg cactacggcc acatacaatc ttccccgctt
     5161 aaatcgatca tccttgaaag aagttgccag tgtcccgttc aggtagacgc ggaatttctc
     5221 tcgatcgcag atcacccgca ggcgattcgt ttcatgcatg ccaggtctca gggccgtgtg
     5281 atttgaccag gcaattaaat cctcccacac cagctcacct ttctcgccct tcacataata
     5341 accaatcgtg taggacccga gggttgagac taacaccgcg tacccaccac cctccggagt
     5401 aaagcgcagg tagaagcccg ctcggatgat attcttgtct ccctcggtaa acttcatatt
     5461 gatgcttgca tcgaagtcgt ccaaaaaccc tgaactcttc aatacataat aggagttata
     5521 gctgggttca tagatgccat gcagttcgga aggatcgccg cgatggaagg ttaaccgttt
     5581 cggttcattg aatacttccc aacccggtat tacatcgtcc ttaaaatccg cgccgagcat
     5641 caagtcaccc atctccggtt cgggcgtcag ggtctttgag attcggaacg tagtaccgca
     5701 gaactcgcac tccaccaggt ccgatttgag cgctcgcgcc ggcagtgcag ctccacagtt
     5761 gggacagtta taagctttca taaggtggaa ggaaaagggt ccatgtccca gaactgcact
     5821 ggtccgttgg atgtatcaat gccgcagtcg ttgccatact gagattctat atcagaatcg
     5881 cctgatggac aacagaaaat catattcaaa atgcttccgc aatggtctga attaggaatg
     5941 ctataattcc gtcaacatca atgaagcact gggacacggc ttcggtgagc gatgcaggcg
     6001 accacttcat ccccgccgcc gcggcgtttg gtggaagctc tgccttcgca tggatgtctc
     6061 aaatgctaca aacgaaatgg ttctctgaag tctcccataa attagtgacc attgagcgaa
     6121 attggatttg gatcatcgcc gcactggttt tcgcgatatt tcacattttt gtcttgggtc
     6181 cgggcgtgga gttataacat gacaaacaga acacctgcta ttatttccgc agtaataacg
     6241 attctcctgc tcgtcctttt ggcagggctt tccgttttta tgcagatgat cgctctaaac
     6301 ggcgcgagtg aacgtcaggg gaccacagca atgagtatct cgctgatttg tcagggcatc
     6361 gggctgatcc ttgcagggct atttgccgga tggctgtcca atttcgtgat caaccgattt
     6421 aattggaata agattctggc agttattatc gcagtgattt tgggagtctt aatcggcggg
     6481 gtcatatccg ttgtgtcttt ttttatttcc cttcccttgg cgggaatcgt ataaattttt
     6541 tatgactcag caggggcgac aaataaaatg tgagtggaaa ccaggtatat aattcctctt
     6601 tcaataaaag gagtgcaaca tgaaagcctt gaccgttctt tttgttcttc tcctattgtt
     6661 ctatgcatgc gtaccgcaac gtaccgagcc tgctccccct cactggacgt atgaaggcga
     6721 agaaggaccc tcgcactggg gcgaacttga ccccggctac gcggtctgtg gaaccggaca
     6781 aagtcaatct ccgattgatg ttgttaacgc ctcagaacag gatctctcga atattgcatt
     6841 ccattatcag cccagcgaag ttaacattct gaacaatggg cataccgtcc aggtcaatta
     6901 cgacactggg agttatatcg aagtagatgg cacacgctat gatgtggtgc aattccatta
     6961 tcacgcgccc agcgagcata cgctggacgg ccaatcattc cctgccgaat tacaccttgt
     7021 ccatcgaaat gcggatggga acctggcagt cgtaggcatt ctgctgaaag aagggatgga
     7081 gaatgctgcg taccaaccct tcatcaacca ccttccccca gaaaaagcag atgcgcaaga
     7141 tgcaggtgtg ccagtcaacg ccggtgactt acttccctct gtagaaacaa ccttccgcta
     7201 tagtggttcg ctcacgacgc ctccctgcac agaaggagtc agctggctgc tgatgaccac
     7261 tccggtagaa ctttctgcga cccaactagg tgcactggat tcgctctttg agggcgggaa
     7321 caaccgcccc acacaaccgc tcaacgatcg ttcgctgctg caggatacga ctccctgaaa
     7381 ttacgggcag ccatagtgaa aatctcgttt ctgaagaaaa acggcaaccg agcgcttggg
     7441 atagagtttt tttggaggat gacaatagaa acagcgtcat cctcggagat cacagaccac
     7501 tttattcccg agttgttttt cgactatttg ttcatcagtg aagggacaat aaaagctgtt
     7561 gaaccatcac gagaaaccga tgtttccctg ccccgggaat gtcttaaaac cattcatacc
     7621 cgtcctacaa catttcttta ttcgactccc tgcgtgttat tcggcgcccg tttctcactg
     7681 cggtttgcag aattattttg ggaaaaagag ctgccatcca attccttcct taagcagaat
     7741 tggatggaaa taaacactaa tgatttagag tcgttcggtg atcaggtcat tcgatatgtt
     7801 caagagcatc aagatcgcaa aacggcgtat ccgatgctga aacacaattt ggatgaatcg
     7861 gattggcttg tccatttttc ttcgagacac aagcgacgct tatacaaaac ggtatttggc
     7921 gtgagcaaaa aggacttgca aaatatccag aacatccatt cctttcttga gcagacctgc
     7981 gacttctctt cggagaaccc gcgcatcatt caatacatta atgcagacgt tttccacgac
     8041 cagccgcacc tgaatcatgc gttcaggaat atgaccggct tctcacccgt ggaatacttt
     8101 gaagccaatt cgatcctgca agacaactta atgtccgctt cttacaatga gtttccatcg
     8161 ggagtagata tactctgaac tatgaagtac acaaaatcca aaaatgacat cgtcaacgaa
     8221 tatatcgacg ccctggatac agcgattgcg cctctttttc tggagatccg agactgtatc
     8281 ctcaaggctg gccccgcctt caatgaaagc atcaagtgga agaattgcct ggtctatggc
     8341 accaccaaaa accacatcca gacggtggtt ggcaagggca agatctcttt gatctttttc
     8401 gagggagttt cgctacagga taaatatgga ctattggaag gggaaggaaa caaggcgcgc
     8461 acgatgcgaa tctcttcacc ggacttcaat aaaaccgctc tgggcgatta cgtcaaacag
     8521 accttgaaaa acgaaacgta gaggatgata ggatgcggaa atatagtgct tcgaccacaa
     8581 tcaacgcctc gccggagaaa atctggagca ttctgacaga cgcaagcagc tacccaacct
     8641 gggatacgag tatggatcgc atcgatggaa agctggcgct cggagagact gtgaaatttt
     8701 ttaccaaatt aagttcacag gcctttccgg ttaaagtaac agggtttgaa cccaatcgcc
     8761 aacttacatt gactggcgga atgccattgg gactgttcaa gtctgagcgc actcaccgac
     8821 tgacttcgaa taaagatgga acaaccacct tccagacgga agaagtattc agcggtttgt
     8881 tgctccccat ctttggcagg accattcccg atctcactga aaacttcaaa gcgttcgccg
     8941 ccggattaaa aaagcaatcc gaaaaataat tccggagagg aaagcaggaa tacgattttg
     9001 cccgcgacga ccctgtgggt ctacccctct gccttcagcg ccgaacgcgt ccacaatgca
     9061 ccaagcagat ccaccgatcc gagcaagatc atgatcggac ccaccaaccc tgcaatcaca
     9121 aacgcggtga acgagataaa tattccaata cgtgcaggga ttgtccattg cgcgaaggca
     9181 cgcaggtccg cacgagctgc tgacatgtag tagaacgcca gtaccaacgc aatcgctccg
     9241 accacgtggg gccatacttc atccgtagcg gggatgccaa agagattcaa tagaatattt
     9301 ggtgtgaata aaaatcctaa tcccattcca atcaaataga tgccaaaaac caaaagcgtt
     9361 gtagacgttt ttttcatgaa ggttctcctt tttggggtta aggtgacagt cacttgcttc
//
LOCUS       DASTJP010000329        12720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1035832, whole genome shotgun sequence.
ACCESSION   DASTJP010000329 DASTJP010000000
VERSION     DASTJP010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12720
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035832"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5664)
                     /locus_tag="VFQ23_09870"
     CDS             complement(<1..5664)
                     /locus_tag="VFQ23_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9906940.1"
                     /translation="MDRKTKRELREKSTAKPSDIQFASIIEKINEGFVALDAQMNYLY
                     INKRGSELLRREPENLIGKNYWDEYPEDKNTSFGQAYLRALETRTAIELEDYYAERDR
                     WFENRVYPSDEGGLSIFFNDITERKRAEAGMRENEERFQTLANGSPILLWVNGLEGSE
                     FVNREYLDFLGLDPQTDVRSYDWSEFVHPEDREAYQNTYLRAFENQTEFSTEFRFRRY
                     DGEYRWMRSEATPRRAADGSFAGYVGASIDITVRKQAELAMQNSEHQLRLITDAMPAL
                     ISYIDSEFRYRFVNQAYRDWFGYENQEVIDRTAMDVLGAAAFERLRPHMEAALAGKTE
                     IFESETPYQRGGTRFIRATYTPDIAADGAINGFFVLVLDITERKQAEEALAEYGRQQA
                     ALYQLTDQLQQSKTLEDTFNAALDAILSALQCQRASILLLDKADVMRFVAWRGLSDDY
                     RTATDGHSPWKPDAKNPEPISMNDIHTAELSDSLKSMIRNEGIGSLAFIPLVFNGKLI
                     GKFMTYFNEPHEFTEREIDLSLTIAHQLAIGIERKREENALRTSEERLRLATQAARMF
                     TWELDIRTQVSTVADNFADALGFSPDLIPKDNEDLLEQFIHPDDRQVILEALTEVVRN
                     HKDVFSMQYRILNPENGQPIWLDVNGKVIYDSNGNAERMFGVAQNITERKDNEQQLIE
                     KARLLDLSNDAIIVRDSTDRIVYWNRGAEQVYGWKHEEVLGKVSHEILQTEHPEPLKQ
                     ILDHLLRDGHWSGELLHTRRDGKQIATFSRWSPDRDSQGQLVSILETNNDITARKQAD
                     RHLAMLADVSELTRQIEDPVELMYAVAKAVGQHLQVKRCLFNETDVERDLEIVHKDFH
                     DGVESVAGEHRISDYSSVTSAEMMAGKTVVNHDSKVDPRTEQDYERTYELSGERAYVA
                     VPLMRENRWVGTLWVSDDEPRQWSQEEVSLLETVAERTWAAVERTRAEEALAKSEAKY
                     RTLFNSMDEGYCVIEFFDGPHGQLSDYVHVEANPAYTANAGIPDIVGKKVREMVPDEA
                     DGWVEIYRNVLITGQPVQFERELVATGRHLELSAFRIEPAERRQVAVMFKNITERKRM
                     ENEKAQLLEREQAQRISAEEAKLEAEQAKAEAERELAERRMAEAALGEWADAPLPQDK
                     RSAGLRYGAAFVATLVAILARLVLDPFLGNALELVTIYAAVAFSVWFGGVGPALLSAL
                     LGYLAISGLIVEPRGVVILDAQTITGFALFLFSNTVVMALGETMRRAQRHAHQSARVA
                     IERQLQAEAQLQEKKLAEASLRQSEALYRAIANGIPGGGVYVVDTDFRYQVVEGSVTE
                     AFELSREMLEGHTVAEVFTKEQAERMEDRLRRNFAGETVDYETRFKDRVYWTQQAPLR
                     DSTEQVLIVTIDITERKLAEEALKRSEERFARFMQYLPGLAWIKDTDGRYIYANEAAS
                     RVFNTPIDELYGKTDEEIFPAEVAAQFRNNDELALMDEKGVQVTETLKHQDGQLHYSL
                     VSKFPIPGPDGSTTLIGGTAFDITERLQAEEALRESEARLRAVFDGTYEYIGLLSPEG
                     TLLEANRAALEFDHNTREEVIGRPFWETPWWAYTPGAQESLRNAIERAANGEPIRYEA
                     PLNRRSGETIIFDFSLHPVRDEQGEVMFLVPEGRNITERKKAEDALYESEERFRAILG
                     QATAGIVRKDAEGRLIFVNQAFCNMLGLTEADLLGKTIWDFTHPDDLEQNKRLYERTM
                     IEGVPFKLEKRLIRADGSIVWVDVSVSPILDVNGKPQSAVAVEVDITGRKQAEHALRE
                     SEERYRALFNSIDEGFCVIEKVTGEGDEPIDFRYIEANPAFAANAGRDNVVGQTIREA
                     YPGEAEEWFQTYDTVLRTGESMRFERGLVTQGRVLELYAFRVKDET"
     gene            complement(5886..6725)
                     /locus_tag="VFQ23_09875"
     CDS             complement(5886..6725)
                     /locus_tag="VFQ23_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9906941.1"
                     /translation="MNVTLWGTRGSLASPGPETARYGGNTSCVEVRGRDGTVLILDAG
                     TGLRGLGLSLPKSIRRVNLLLTHLHMDHIQGLGFFGALRNPEMEVHIWGPASTTLDLR
                     SRLTRYLSPPLFPVHLRDLPSTLFFHEVPQEDFDIGEFHISTALVCHPDPTVGYRIQS
                     PDAVITYLPDHEPALGAKTFPLSPDWTSGYELAAGSDILIHDAQFTEDEYKTRVGWGH
                     SSLAQAFEFAELAEVKSFIPFHHDPSHTDDILDGLINEAIGWAQPGLRVLPGIEGATF
                     EAG"
     gene            complement(6722..7117)
                     /locus_tag="VFQ23_09880"
     CDS             complement(6722..7117)
                     /locus_tag="VFQ23_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906942.1"
                     /translation="MSSQKEIELILARQLASHLAMPIFIVDPPGNLIYYNEPAEIILG
                     RRFEETGEMPVDQWSTIFNPSDENGNPLAPEALPLVIALTQHCPAHRAMWINGLDGVR
                     RRIEVTAFPIDGQAERSLGALAIFWETKQ"
     gene            7272..8084
                     /locus_tag="VFQ23_09885"
     CDS             7272..8084
                     /locus_tag="VFQ23_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HET9906943.1"
                     /translation="MTIWRLLLTPPARGAWNMAVDESILEHIGEGESLPTLRLYAWEP
                     ACLSLGHAQPVSDVDADRLKARGWEVVRRPTGGRAILHTDELTYSVIAPSDEPTVTGT
                     VLESYNRLAQALLLAVKYMDLSVEMKEGKADGNSTPNPICFEVPSTYEITVDGKKLIG
                     SAQARKKEGVLQHGSLPLTGDLTRICQVLVFQGEAAREEASKRLLTRAVTVESALGRV
                     VSWGIAAQSFIRAFQKQLGLSLERGELSESESKRADELVKEKYDHPSWTERV"
     gene            complement(8112..8783)
                     /locus_tag="VFQ23_09890"
     CDS             complement(8112..8783)
                     /locus_tag="VFQ23_09890"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HET9906944.1"
                     /translation="MKNNNLLSLLILVVIVVAAYFIVQAVRQGAQLATQPFQQVNEAN
                     QALQTQMSSLMNPTPTIIPDPVTYINEIRALARLETIQYSIEKVITGELGGGTFQALF
                     GDKILFVAHGTVIAGIDLQKLNPEDMRFENGVLTVKLPPTEVLIATLDNEKSYVYDRQ
                     TGVLNKPDPNLETQVRQVAEQEILKAALEDGILEQAQINAEAYLLKFFAALGYPNTIF
                     VYDQG"
     gene            8823..9893
                     /locus_tag="VFQ23_09895"
     CDS             8823..9893
                     /locus_tag="VFQ23_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HET9906945.1"
                     /translation="MNRRRNLLPLILLTASFLLADCTIPSIRDLRDSTPTPTIVQTNT
                     PDAQTALTTIPVTPTPLPPVPIAELGLAENPLILALPPAANTQEQVTAAQQIAAQITE
                     RTGYTVVTIVPETYTDLLTALANGNADIVLLEPYAYELAYRQDLVRASYAVIRDGEGK
                     YGAQFIAARGSGFESYFNPLNETNIISDARIAITQLANKKPCWSDETSASGYVIPLGY
                     LNMYQLNTRPAAFVGGHPTVVRSLYATGICDFGATYVDARKFPSLEDEYPDLVEQVVV
                     IWRIPKIIPHEVLAFSTRMSQEMRDQISAIIPAILQTEAGEAAFKTAYDIDEIEPVND
                     GYYEEFRIYLDESRVDLTTLVK"
     gene            complement(9966..10115)
                     /locus_tag="VFQ23_09900"
     CDS             complement(9966..10115)
                     /locus_tag="VFQ23_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET9906946.1"
                     /translation="MFFISEERGQGLVEYAIILAFVALVVISVVRLLGPRIGNTFSNI
                     NNSIP"
     gene            complement(10264..10413)
                     /locus_tag="VFQ23_09905"
     CDS             complement(10264..10413)
                     /locus_tag="VFQ23_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET9906947.1"
                     /translation="MLFAPKEKGQGLVEYAIILALVAIVVIAVMRLLGPKIGNTFSTI
                     SSSLP"
     gene            10844..11506
                     /locus_tag="VFQ23_09910"
     CDS             10844..11506
                     /locus_tag="VFQ23_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HET9906948.1"
                     /translation="MMHFLKRFFRFLWRFVLAVGMLGVLGVILPRLITTIYSMNRIYQ
                     KDDAPSERLAIVFGAGLRRDGTPTPILRDRVETAASLYFSGRVEKLLMSGDNSFLNYN
                     EPESMRQYAISLGVPEDAIAMDYAGRRTYDTCYRAKAVFGMQSALLVTQKFHLPRALF
                     LCNALGLKAYGVEANNRNYRDGALFIWNFREQLATVGAFLDVYVSSPLPVLGEPEPLF
                     VD"
     gene            12050..12310
                     /locus_tag="VFQ23_09915"
     CDS             12050..12310
                     /locus_tag="VFQ23_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HET9906949.1"
                     /translation="MDMIKVSANSRTSAVAGAIAGVIREHKRAEVQAIGAGAVNQAVK
                     ALVLASGYLKNDGIDVVCVPEFVDVTIDDKVRTAIKLVVVPR"
     gene            12480..>12720
                     /locus_tag="VFQ23_09920"
     CDS             12480..>12720
                     /locus_tag="VFQ23_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906950.1"
                     /translation="MRFSQRFWRGLVFLLFIAALCGGMVLPAASIGAAPLMQATACVG
                     TTITQWTFAGDVVTPSTGSGTYLVGPGLPATTFAAG"
BASE COUNT         3120 a         3197 c         3026 g         3377 t
ORIGIN      
        1 cgtttcgtcc ttaacacgaa atgcatagag ttccaataca cgtccctggg tgaccaggcc
       61 ccgctcaaat ctcatggatt ctcccgttct gagaacggta tcatacgtct ggaaccattc
      121 ttccgcctca cccggatatg cctcccgaat cgtttgccca accacgttat ctctgccagc
      181 atttgccgcg aaggccgggt tagcttcgat gtagcgaaaa tcgattggct catcgccttc
      241 accagtaact ttctcaataa cgcaaaagcc ttcatcaatc gagttgaaca aagcgcgata
      301 gcgttcttct gattcgcgta acgcatgctc ggcttgcttg cgccctgtga tatcaacttc
      361 cacagcaaca gcagattgtg gcttcccgtt tacatccagg atcggagaaa cactgacatc
      421 cacccacaca attgacccgt ccgcgcgaat gaggcgtttt tcgagcttga atggcacacc
      481 ttcgatcatc gtgcgttcat acaaacgttt gttttgttcc aggtcatccg gatgggtaaa
      541 atcccagatc gtttttccaa gcaagtcggc ttcggtgagt ccaagcatat tgcagaaagc
      601 ctgatttaca aatatcaatc taccctcggc atccttacga acaatccctg cggtagcctg
      661 cccgagaatt gcgcgaaaac gttcttctga ttcatagaga gcatcctctg cctttttgcg
      721 ttcagtgata ttgcgtcctt caggcacaag gaacattact tccccttgct catcgcgaac
      781 gggatgtaac gagaagtcaa atatgatcgt ttctccagag cggcgattga gtggcgcttc
      841 gtaacgaatg ggctcgccgt tggccgcacg ctctatggca ttgcgcaatg attcctgcgc
      901 acctggcgta taggcccacc agggggtttc ccaaaatggg cgtccaatta cttcctcgcg
      961 agtgttgtgg tcaaactcaa gtgccgcacg gttggcttcg agcaaggtac cttcgggaga
     1021 gagtaacccg atatattcat aggtgccatc aaaaacggct ctcaacctgg cttcgctttc
     1081 gcgcagcgct tcctccgcct gtagacgttc ggtgatatca aatgccgtcc caccaattag
     1141 agtggtgctg ccatccggtc cggggatggg aaacttgcta accaatgaat aatgtagctg
     1201 gccgtcttgg tgcttcaacg tctctgtaac ctgcacacct ttctcatcca tgagcgccaa
     1261 ttcgtcattg ttccggaatt gggctgctac ctccgcaggg aatatttcct catcagtttt
     1321 gccgtagagt tcatcaatgg gtgtattgaa caccctggaa gcagcctcgt tagcataaat
     1381 gtaacgaccg tctgtatctt tgatccacgc cagcccaggg aggtattgca taaatcgggc
     1441 aaaacgttcc tcgcttcgtt tcaatgcctc ttctgccagt ttacgttccg taatatcaat
     1501 ggtcacaatc agcacctgct ctgtcgagtc acgcaatgga gcctgttgag tccaataaac
     1561 gcgatctttg aatctcgtct cgtaatccac cgtttcacca gcaaagtttc ttcgtaggcg
     1621 atcctccatt ctttccgcct gctcttttgt aaagacctct gcaacagtgt gaccttcgag
     1681 catctcgcgg gaaagctcaa atgcctccgt cacggatccc tccaccacct ggtaccggaa
     1741 atctgtatca acgacgtaga ctccgccacc aggaatgccg ttggcaattg cgcggtagag
     1801 agcctcactt tgacgtaagg atgcctcagc gagctttttc tcttgcaatt gcgcctcggc
     1861 ctgaagctgt ctttcgatgg ccacacgagc gctttggtgc gcatgacgct gggcgcgtcg
     1921 catggtttca cccagcgcca tcaccaccgt attgctaaat aagaagagtg cgaaccctgt
     1981 tatggtttgt gcatccagga taaccacgcc ccgcggttct acgatgagtc ctgaaattgc
     2041 caaatatcca agaagcgcag aaagcaatgc ggggcctacc cctccaaacc agacactaaa
     2101 agctaccgct gcatagattg tcacaagttc cagggcattg cccagaaacg ggtcgagcac
     2161 cagacgcgcc agaatagcca caagcgttgc aacaaacgcg gcaccataac gaaggcctgc
     2221 agatcgtttg tcctgaggca gcggggcatc tgcccactcg ccgagtgcag cttctgccat
     2281 tctccgttct gctaattccc gctccgcttc agctttggct tgttcggctt ccagcttcgc
     2341 ctcttctgca ctgatgcgct gcgcctgctc acgctcgagc agttgagctt tttcattttc
     2401 catccgttta cgctcggtga tgtttttgaa catcacggcg acctgtcgtc gctccgcggg
     2461 ttcgatccga aacgcggata gttcgaggtg tcttcccgtg gcgaccagct cgcgctcgaa
     2521 ttgtacgggt tggcctgtga tcagtacgtt gcggtagatc tcgacccagc cgtcggcttc
     2581 gtcgggcacc atctcacgca ctttcttccc gacgatatcc gggatgcctg cgttcgctgt
     2641 gtacgccgga ttggcctcga catgcacgta gtcgctcaac tgcccatgtg ggccatcgaa
     2701 aaactcaatg acgcagtagc cctcgtccat cgagttaaac agggtgcggt attttgcttc
     2761 cgatttggct aatgcttctt cggcgcgggt gcgttctact gctgcccatg tgcgctccgc
     2821 gacggtttcg agcagagaaa cttcttcctg actccactga cgtggctcgt catcacttac
     2881 ccacaatgtc cccacccacc gattttcacg catcaaggga accgccacat aggcccgttc
     2941 accactgagt tcataagttc tttcataatc ctgttcagtg cgtggatcaa cttttgagtc
     3001 gtgattaaca acagtttttc cagccatcat ctcagcagaa gtaacgctgc tgtaatctga
     3061 aattctatgt tctccagcta cagattcaac gccgtcatga aaatccttgt gaacgatctc
     3121 aagatctctt tcaacgtccg tttcattaaa cagacagcgt ttcacctgaa ggtgctgacc
     3181 gaccgctttc gcgactgcat acatcaattc aacggggtct tcgatttgcc gggttaattc
     3241 actaacgtcc gcaagcatgg caaggtgacg atctgcctgc ttacgtgcag taatatcatt
     3301 gtttgtttcc aaaatcgata ctaactgtcc ctgtgaatca cgatccgggg accagcgtga
     3361 gaaggtagcg atctgctttc catcgcgacg ggtgtggagg agttccccag accagtgtcc
     3421 gtcacgaagc aaatggtcaa ggatctgctt caaaggttca gggtgctcgg tttgaagaat
     3481 ttcatgagag actttaccaa gcacttcttc atgtttccat ccatatactt gttctgcgcc
     3541 acgattccaa tatacaatcc gatccgtgga atctcgtacg ataatggcat cgttgctcag
     3601 gtcaagcaga cgagctttct caatgagttg ctgctcattg tctttacgct ccgtaatatt
     3661 ttgcgctact ccaaacatcc gttccgcgtt gccattagag tcgtaaatta cttttccatt
     3721 cacatccagc cagatcggct gaccattttc aggattcagt atccggtatt gcatggaaaa
     3781 cacatctttg tgatttcgca caacttcggt aagtgcttcc aaaataactt gtctatcgtc
     3841 cgggtgaatg aactgttcca ggagatcttc attgtccttc ggaatcagat ccggagaaaa
     3901 acccagggca tctgcaaaat tatccgctac cgtggaaacc tgcgtacgaa tatcaagttc
     3961 ccaggtaaac atccgggcag cctgggtagc aaggcgcagg cgttcttctg aggtacgcag
     4021 cgcgttctcc tctcgtttgc gttcaatccc aatagcgagc tggtgagcaa tcgtgaggct
     4081 gagatcgatc tctctttcgg tgaactcatg aggctcatta aaatacgtca tgaacttgcc
     4141 aatgagtttt ccattgaaga ccagcggaat gaatgccaac gaaccaatgc cttcgttcct
     4201 gatcattgat ttgagcgaat cgctgagttc agctgtgtga atatcgttca tacttatcgg
     4261 ctcagggttc ttcgcatctg gcttccacgg cgaatgacca tctgtagcgg tacgataatc
     4321 atctgataaa ccgcgccagg caacaaatcg catcacatcg gctttatcaa gaagcaaaat
     4381 tgaagcgcgc tggcactgca aagcgcttaa aatggcgtcc agggccgcat tgaatgtgtc
     4441 ttccagggtt ttcgattgtt gaagttggtc cgttaactgg tacagcgccg cttgctgacg
     4501 cccgtattca gcgagcgctt cctccgcttg tttacgctca gttatgtcaa ggacgagaac
     4561 gaaaaagcca ttgatcgcgc catcggctgc gatatcgggc gtgtacgtgg cgcgaataaa
     4621 gcgcgtgcct cctcgttggt atggagtttc gctttcaaat atctcagtct tgccagccag
     4681 cgccgcttcc atgtgcgggc gtaatctttc aaacgccgcc gccccaagca catccatcgc
     4741 tgttctgtcg ataacttcct gattttcata gccgaaccaa tctcgataag cttgattgac
     4801 aaaccgataa cgaaactcgc tatcgatata agaaatcaat gcgggcatcg catctgtaat
     4861 cagccgaagt tgatgttcgc tgttctgcat ggcgagttcg gcctgcttac gaactgtaat
     4921 atcaatgctc gctccaacat agcctgcgaa tgatccatcg gcagcgcggc gcggcgtggc
     4981 ttctgagcgc atccaacgat attcgccgtc ataccgacgg aaacggaatt cggtgctgaa
     5041 ttctgtctgg ttttcgaaag cacgcaagta tgtattctga tacgcttcac gatcttctgg
     5101 atgcacaaat tcactccagt cgtagctgcg tacgtcagtc tgaggatcaa gaccgagaaa
     5161 atcaagatac tcgcgattga caaactcgct gccttccaac ccattcaccc aaagcaggat
     5221 cggtgaacca ttggcgagag tctgaaaacg ctcctcgttc tcgcgcatgc ctgcctcggc
     5281 gcgtttgcgc tcggtaatgt cattaaaaaa gatggagagc ccgccttcat cacttggata
     5341 gacacgattt tcaaaccaac gatctcgctc ggcatagtag tcttcgagtt caatcgcggt
     5401 tctcgtttcc agcgcgcgca agtaggcctg accaaaagat gtatttttat cttcggggta
     5461 ttcgtcccaa tagtttttac cgatcaagtt ttctggctcg cgcctcagga gttcacttcc
     5521 ccgcttgttg atatagaggt aattcatctg cgcgtccaac gccacaaaac cttcgtttat
     5581 cttttcaatg atgctggcga attggatatc tgagggcttc gcggttgatt tttcacgaag
     5641 ctctctcttg gttttgcgat ccattttcat agacggtcct tctatttttt aaatggcaaa
     5701 agcctcagaa aacagccctg cttcctgata gttcacattc aaactgttca agattattat
     5761 aatgttttca aattggatta acattcactt agtagggaaa tccctaccgt cagcaatatt
     5821 attataatgt tttcaacgct cacaaatggc ttttttactg gtggattccc acaattggcc
     5881 gttgcctacc ctgcctcaaa cgtcgcgccc tcgataccag gcaataccct aaggccaggc
     5941 tgagcccaac caatagcctc attaatcaga ccgtccagga tatcatccgt gtgagatggg
     6001 tcatgatgaa aagggataaa ggatttgact tctgctaatt ccgcgaattc gaaggcctgt
     6061 gccagcgagc tatgtcccca accgacacgc gtcttatatt catcctcagt gaactgcgca
     6121 tcatgaatca aaatatcact cccggccgca agttcatatc cagacgtcca atctgggctg
     6181 agtggaaatg ttttcgcacc cagggcgggt tcgtgatctg gcaaatatgt aatgaccgca
     6241 tctggagatt gaatgcgata accgacggtt ggatcgggat gacatacaag agcagtagag
     6301 atatgaaact caccgatatc gaaatcttcc tgaggcactt catgaaagaa aagcgtactt
     6361 ggcagatccc gtaaatgaac tggaaacagg ggtggtgaca aatatcgggt gagacgcgag
     6421 cgcaaatcga gggtcgtact tgcaggtccc cagatatgaa cttccatttc agggttgcgc
     6481 agcgctccaa aaaaaccaag cccctgaata tgatccatat gcagatgagt cagcaaaaga
     6541 ttcacacggc ggattgattt cggcagtgaa aggccgagcc ctcgaagtcc agtgcctgca
     6601 tccagaataa gcacagtgcc atcccggcct cgaacttcca cgcaagaggt attgcctcca
     6661 taacgggcag tctctggtcc tggcgatgcg agcgaaccgc gcgtgcccca caaggtgaca
     6721 ttcattgttt cgtttcccag aatatggcga gcgcccctaa agagcgctcc gcctggccat
     6781 cgattgggaa agccgtgact tcaatccggc ggcggacgcc atccagaccg ttgatccaca
     6841 tggcgcgatg ggccgggcaa tgctgcgtca gtgcaatcac aaggggtaac gcttcaggtg
     6901 caagtggatt gccattttca tcacttggat tgaagattgt ggaccattgg tcaaccggca
     6961 tttcgcccgt ttcctcaaag cggcggccaa ggataatctc ggccggttca ttgtaataaa
     7021 tgagatttcc tggcggatct acaataaaga tgggcatggc aagatgactg gcaagttgtc
     7081 gcgcaaggat caactcaatc tctttttgtg aggacatatc tgtctgtttc cttcaagtta
     7141 taaatgacag tattactttc gaatcggtct gccctcattg attttgagga ttaattacaa
     7201 attaagataa ttgtacacgt tatttttcta tttattaagt ggggagatac cttatttagt
     7261 acaataagtt catgacaatt tggagactcc tcctcacccc gcccgcgcgc ggtgcctgga
     7321 atatggctgt agatgaatcg atcctcgagc atattgggga gggagagtcc ctaccgacgt
     7381 tgcgactgta tgcgtgggaa ccagcttgtc tctcactcgg gcatgctcaa ccagtttctg
     7441 atgtggatgc agatcgtttg aaagcgcgcg gctgggaggt tgtgcgtcgc ccaaccggcg
     7501 gacgcgcgat attgcatacc gatgaattga cgtattccgt tatcgccccc agcgatgagc
     7561 ctactgtaac aggcactgta cttgaatcat acaaccgact tgcgcaggcc ttgttgctgg
     7621 cagtgaaata catggatctg tctgtggaaa tgaaagaagg gaaagcggat ggaaattcaa
     7681 ctccgaatcc gatttgtttt gaagttccat ccacctatga aattacagtt gacgggaaaa
     7741 agctgattgg ttcggctcag gctcgcaaaa aggaaggtgt actccaacat gggtccttac
     7801 ccctgaccgg cgacctgacg cgcatttgtc aggtgttggt ttttcaaggt gaagccgcgc
     7861 gcgaggaggc ttcaaagcga ctcctgacac gagccgtgac cgtcgagtct gcgttggggc
     7921 gtgttgtcag ttggggaatt gcggcacagt ccttcatccg cgcgtttcaa aagcagttag
     7981 ggttaagcct cgaaagggga gaattatctg aatccgaatc gaaaagagca gatgaattgg
     8041 tcaaagagaa atatgaccat ccatcatgga ccgaacgcgt gtaggggaga gcagcgcact
     8101 tgcttacacc atcatccctg atcgtataca aagatggtgt tggggtaccc aagcgcggcg
     8161 aagaatttga gtaaataggc ctcggcgttg atctgtgcct gctccagaat accatcttcc
     8221 agtgctgctt tgagaatctc ctgctcggct acctgacgta cttgagtttc aaggttgggg
     8281 tcaggtttgt tcagtacacc ggtctgacgg tcatacacat aggatttttc gttatcgagc
     8341 gtcgcaatca atacttccgt tggcgggagc ttgacagtaa gtacgccatt ttcaaaacgc
     8401 atatcttccg ggtttagttt ttgcaggtcg atacctgcaa tgacggttcc atgtgcaacg
     8461 aataaaatct tatcgccgaa cagtgcctgg aacgtgcctc cacctagttc gcctgtaatc
     8521 actttttcga tgctgtactg aatcgtttcc agccttgcca gcgcgcgtat ttcattgata
     8581 taggtaacgg ggtcagggat gattgttggc gttgggttca tcagactgga catttgggtc
     8641 tgcagcgcct gattggcctc attcacttgt tgaaatggtt gtgtagccag ttgtgcaccc
     8701 tggcgtaccg cctgtacgat aaaatacgcc gcgacaacga tcaccaccag gatcaacaaa
     8761 gaaagcaggt tattattttt catgcacgta ttataatctc attggggaac gagtgaaaca
     8821 gcgtgaatcg tcgtagaaac ctcctgccct taatcctatt aaccgcttcc tttttgttag
     8881 cggattgtac gattcccagt attcgcgatt tgagggattc aactccgact cccactatcg
     8941 ttcagaccaa caccccagat gctcaaaccg cgttgaccac gatcccagtc acaccaaccc
     9001 ctctcccacc tgtgccgatc gcggagcttg ggcttgcaga aaacccattg atccttgctc
     9061 tgcctccggc tgccaacacc caggaacagg tcaccgctgc ccagcagatc gctgcccaga
     9121 tcacggagcg aaccgggtat accgtcgtca cgatcgttcc cgaaacatat actgacctcc
     9181 taacagctct tgccaatggc aatgcagaca ttgtccttct cgagccctat gcgtatgaac
     9241 ttgcctatcg gcaggattta gtgagagcgt cttatgccgt cataagagat ggcgaaggga
     9301 agtatggcgc gcaattcatt gcagcgcgcg ggtctgggtt tgaatcgtat ttcaacccct
     9361 tgaacgaaac gaacatcatt tctgatgcgc ggatcgccat cactcaactg gcaaacaaga
     9421 agccctgctg gagcgacgag acatctgcct cagggtatgt cattcctctg ggatacctca
     9481 acatgtatca actcaatacc cgaccagccg cctttgttgg tggacaccca actgtggtgc
     9541 gctccctgta tgccactgga atctgtgact tcggtgcaac gtatgtagat gcacgcaagt
     9601 ttccatcact cgaggatgaa tatccggacc ttgtggagca ggtcgttgtg atatggcgca
     9661 tcccgaaaat tattcctcat gaagtgctgg cgttttccac acggatgtct caggaaatgc
     9721 gcgatcagat ttctgccatt attcccgcca tcctgcaaac cgaggccgga gaagcagctt
     9781 tcaagactgc gtatgatatc gatgaaatag aaccggttaa tgacggttac tatgaggaat
     9841 tccgtattta tctcgatgag tcacgagtgg acttgaccac gttggtgaag tgagggaata
     9901 cagagtagtt cgaagataaa aaagagccct gcctttcgac agggttcttt tcttgcggtc
     9961 tgagttcatg gaatggaatt gttgatatta ctaaacgtat ttccaatcct tggacccagt
    10021 aatcgtacga cactgatcac gacaagtgct acgaaagcga ggatgatcgc atattcgacc
    10081 aggccctgac ctctttcctc tgaaatgaaa aacataaaat aaacctttca aataaatggg
    10141 ccagattccc tgagtacctc ctcagagtcc aacgtattca attttagtga aattaaatcc
    10201 ccaataggtg atacaaaaaa gtcccgccaa attgacggga ctttgtttgc tcttacgagc
    10261 gggttacggc agggagctcg aaatggtgct gaaggtattg ccgatcttgg gtcccagcaa
    10321 acgcataaca gcaatcacga cgatggctac gagcgcaaga atgatcgcgt actcgactaa
    10381 gccctgacct ttttcttttg gtgcaaacaa cattggagta attcctttct aaaaaaaaat
    10441 tggtttcacc tgaggaatgc ctcagaacta tatacagtat aaccagttcg cacaaaattt
    10501 caagccctat atccattatt tcgtttacaa gtttgcaaat attaagggct tctcataaac
    10561 cttgtaacac aatgaaaaca aaagatcagc tactattttt gattatttat atttcgactt
    10621 gacgaaatat ggaaaattac agaatacatt atggatgtct gacatattca tgcatcgatg
    10681 cgtaaagact tgtttacaaa acgattagaa aacttaatta ttattaggac aagggttaaa
    10741 atttcacctt atgttatctc ttatcttagt agatggaact ttcctcgttt tgttgaatgc
    10801 caattttttt agaaaacatt atgcgtttgg agtttagatt ttaatgatgc actttttaaa
    10861 acgatttttt cggtttttat ggcgttttgt cctggcagtg ggaatgttgg gcgtactagg
    10921 cgtgatcctg cctcgtttga taacaaccat ttattcgatg aatcgcatct accaaaagga
    10981 tgatgcgcca agcgagaggc ttgcaattgt atttggtgcc gggttacggc gcgacggaac
    11041 gcccaccccg attttgcgag atcgggttga aacggctgcg agcctttatt tcagcggcag
    11101 agtggaaaaa ttgttgatga gtggggataa tagtttcctg aactacaatg agccagaatc
    11161 gatgcggcaa tatgcgattt cccttggcgt tccagaggat gcaatcgcta tggattatgc
    11221 cggccggcga acctacgaca cctgctatcg agcgaaagcc gtattcggga tgcagagcgc
    11281 cctgctcgtg actcaaaaat tccatcttcc acgcgcttta ttcctgtgta atgcactggg
    11341 gttaaaggcc tatggcgtag aggcaaataa ccgaaattac cgggatgggg cactatttat
    11401 ctggaatttc cgcgagcaat tggctacagt gggggcattt ctggatgtat acgtgagcag
    11461 cccgctacct gtcctgggtg aaccggagcc tttgtttgtg gattgattga tcatcccaca
    11521 cccccaactg ctctcaagtg tgggaatatc ctatagtact atcggcacat ttaggcaatt
    11581 aaagtcatat taatcctact gcgtgagctt gtacagacaa gcgttttgtg gataagtccc
    11641 ctataactgt ggataagtgc aacttcatgt ggagaagtat ggaaaaccca tcaaatctgc
    11701 aagaagacgt acccccaaat atggacgcga ataattagag tctccaatat atggaattgg
    11761 tccaaaagtg tcctcaggtg tggataaaga tgtggataag ttattccgac tctttccaca
    11821 ggcgggttag gtctggtgag cggtgccaag cccaatatcc agagtgactc gcaggtcttc
    11881 atcctatatg tgggggatat tttttcggat tttgttctat ccacatttcc acagtcccta
    11941 ctgctattac gattcttagt ttatatttat ataaatgatt gaatctaatt ccataatcct
    12001 gtagaatctt tccacaagtc tacatcgtta acattgctgg gaggttaaca tggacatgat
    12061 caaggtttct gcaaactccc gaacctctgc cgttgccggt gcaattgcag gagtgatccg
    12121 tgagcataaa agggcggaag tacaggcgat tggcgccggg gcggtcaatc aggcagtgaa
    12181 agcgctggtg ctcgcttctg gttatctcaa gaatgatggt attgatgtgg tctgtgttcc
    12241 cgagttcgtg gatgtgacca ttgatgacaa agtgagaacg gccatcaagc tggttgtcgt
    12301 acctcgttga aatgtaaagt gaattaaagc aggttcgata agcggactga attgataagg
    12361 tacgtttcac ctctcaaatt cataatgtca gagtaagtgc agtttgtatt gtaatcaacc
    12421 agggtggtat ataatttacc ggactgtgtt tttaacagct gccatcaaag gtgatttctt
    12481 tgagattctc acaacggttc tggcgcggtc tggtatttct cctattcatt gccgctttgt
    12541 gcgggggtat ggtgttgcct gccgctagta taggtgctgc gccattaatg caggcaacag
    12601 cctgtgttgg tacgaccatt acacaatgga catttgccgg cgatgtagtc acgccttcta
    12661 ctggctcagg aacatattta gtgggcccag gcttacctgc tactacattt gcagctggtg
//
LOCUS       DASTJP010000330         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_435593, whole genome shotgun sequence.
ACCESSION   DASTJP010000330 DASTJP010000000
VERSION     DASTJP010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435593"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            916..2904
                     /gene="dhaR"
                     /locus_tag="VFQ23_09925"
     CDS             916..2904
                     /gene="dhaR"
                     /locus_tag="VFQ23_09925"
                     /inference="COORDINATES: protein motif:HMM:NF008485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HET9906951.1"
                     /translation="MDQPQDLPNENDRRKRAWQQFTSTGKIVEDAGLNSLVMASWMRC
                     SLRMNPNGLLQWTYASDNALQSTLKQHTTLRNIARPIMEDIYEFLENCRVTLILTDSS
                     GCLLEMLGEPSFTSELQTLGFRLGAYLDEGHLGTNAIAVTLFEAMPSQIVGAEHFSIH
                     LHGLSSVAAPVHTIEGHPLGVLGLLEPVEQYSHQSFGVVVAGARAIENQLRADLIVRD
                     ANAKTAELYATMDAITEGVLAWSNDGVVMHLNDQAGRTLHLSPTTVVGRPMAEFFSLP
                     ENIARSVALGQELSDIETAFVVDGTPRECLVSLRLISSQENEWVYIMTLRRIEQVHQL
                     VNRLVGARARLTLDDIVGHGAAAKKIRKQALAAANAEACVILTGEAGTGKNILARAIH
                     NSGRRAGGPFLAINCRAIPRELALGEFLGFEAGAFNTGASLGQPSKFELAQGGTLFLE
                     EIESLPLETQAALQRVIEMGDVIRLGGNRVIPVDVRIIVSTKRSMDELLELDSFRHDL
                     LLRLSSFVITMEPLRKRAEDIPLLVEHLLDKLTVQLNRPLKISAEVLQVLTEYPWPGN
                     IREVETVMERAALLFEGPQIELDNLPPAIAKRSALVKGKSVVQPIQTLEQAEKAAILI
                     ATQAANGNLTKVAQSLGISRTTLWRKMKELGISMESQS"
BASE COUNT          795 a          754 c          829 g          757 t
ORIGIN      
        1 atccagacgg agaacggctg gcagtcggcg ccgtttcggg tgtattcgtt tttgtcctgc
       61 caaagaacga cctgatcgaa ctggcacagt cgcgtctgac tcgctctcta acccctgagg
      121 aatgtcaaca gtatttacat ctggcaacat gccctacgag accctaaacc cgaatgggct
      181 atcacccttg cctttatgtg aaagagtttg gaaaaagaga atgccaactt gaggagattg
      241 atggccgatc tgtgaccaga taacggcatg ctgaaagaga cgctcaagag aaacttctac
      301 taaaattttg gatgcaaagg acatttagtc ccttaaaaat gccaaaatcc ggacaaagac
      361 gcacaaaacg gatgtattgg aaggtgagcc tttagaatca cacagtagca ggctacagtc
      421 caggcatata gttctactac gtgataaaat gaaataacaa aagcaatcac tgagcgccga
      481 cgtcgttacg cgtcgcgaac cctaatcccg tcgggaccgc ctctataaac aacttgatga
      541 tttcgtcaag ttgtttttgt tttcaatctc ttgaagcatt ctatttcact aatgttgaat
      601 gcggatgcaa tgcttggtat tggatctcaa ctcgagcaac aggctggaaa tatgttcaag
      661 cactgagtag caacagcagg tgcaccatac aggactttat ttatgtcaag aatttgcgca
      721 tgggcagggt tgtatacagg aagactaatc tctctttttc aaaccaggac aaacaaattg
      781 tgtgaagcaa attttctggt ttctgtctgc ggttcattcc cctaaggttt tcaacgtaga
      841 atttcctcaa tacccttgac tcctagattg aagttatact tgtaggaacg tcccttaaat
      901 gtaaacgata tcagcatgga tcaacctcaa gatctcccca acgaaaatga tcgccgaaaa
      961 cgcgcctggc aacagttcac ttcgaccgga aaaatcgtcg aagatgccgg cctcaattct
     1021 ctggtcatgg cgtcctggat gcgttgcagc ttgcggatga atccaaacgg tctgttgcag
     1081 tggacgtatg ccagcgacaa cgctctgcaa agcaccctga aacaacatac gacactccgc
     1141 aatattgccc gtccaatcat ggaggatatc tacgagttcc tggagaactg ccgtgtaact
     1201 ttgattctca cggatagctc cggttgtctg ctcgagatgc ttggagaacc ctctttcact
     1261 tccgaattgc agacgctggg atttcgactg ggtgcttatc tggatgaggg acatcttggg
     1321 accaatgcga ttgccgtgac attgttcgaa gccatgccct cccaaatcgt gggtgccgaa
     1381 catttttcaa tccacttgca cggtctaagc tcggtggctg ccccggttca taccatcgaa
     1441 gggcatccgc tcggagtgct tggattgctg gaaccggtcg aacagtacag tcatcaatcc
     1501 tttggcgtcg tggtggcagg ggcgcgggca atcgagaatc aactccgtgc agacctcatt
     1561 gtccgtgacg caaatgcaaa aactgccgag ttgtatgcga cgatggacgc cattaccgag
     1621 ggtgtgctgg catggtctaa cgacggagtc gtgatgcact tgaacgatca ggcgggaagg
     1681 accttgcatt tatctcccac cactgtagtc ggtcgtccga tggctgaatt cttttccttg
     1741 cctgagaaca ttgcccgctc tgttgcgttg ggacaggaat tgagcgacat cgaaactgcc
     1801 tttgtcgtag atggaacgcc gcgcgaatgt ttggttagct tgcggctcat ctcttcgcag
     1861 gaaaatgagt gggtctacat catgaccttg aggcgcatcg aacaggtgca tcaactggtc
     1921 aaccgattgg taggcgcgcg ggcgcgtttg acattggacg acattgtggg tcatggcgca
     1981 gcagcgaaga agatccgcaa acaggccctg gctgctgcca acgccgaagc ctgtgtgatc
     2041 ttaacaggcg aagccggtac cggcaaaaat atcctggcgc gtgccatcca taatagtggt
     2101 cgacgggctg gtggaccgtt cctggcgatc aactgtcggg caatcccgcg cgaattggcg
     2161 ctgggagagt ttttggggtt cgaagcaggc gcgttcaata cgggtgcatc ccttgggcag
     2221 cccagcaagt tcgaactggc gcagggcgga acattgtttt tggaggaaat cgaatcgctc
     2281 ccgttggaga cacaggctgc cctccagcgc gtcatcgaga tgggagatgt gatccggctg
     2341 ggtgggaacc gcgtcatccc ggtcgatgtg cgtatcatcg tctccaccaa gcgcagtatg
     2401 gatgaacttc tggaattgga ctcattcagg cacgacctgt tgttgcggtt aagttcgttc
     2461 gtgatcacca tggagcctct acgcaaacgt gctgaggata ttccgcttct ggtcgaacat
     2521 ctattggata aactgacggt tcaattgaac cgtccgttaa agatttctgc ggaggtactc
     2581 caggtgctga cggaatatcc gtggccggga aatatccgtg aggtcgaaac ggtcatggaa
     2641 cgagcggcgc tattgtttga aggtccacag atcgagttgg ataacctgcc gcctgcgatc
     2701 gcgaaacgga gtgccctggt gaaggggaaa tccgtggtgc agccgatcca gacgctcgag
     2761 caggctgaaa aagcggcgat tctaatcgct acccaggcgg caaacggcaa cctgacgaaa
     2821 gttgcccaat ccctgggcat tagccgcacg acgctgtggc gaaagatgaa ggaattggga
     2881 atttcaatgg agagtcagag ctagtgtttc aaaatgaaac gctacgtcaa aagtcctgtt
     2941 gcaaaataaa acaaaagttt cggatttttt gaaaaatctg cgggggtgac ataacatgaa
     3001 tacatgatct tgtatttcca acagcaacgt tcacagcaat tcgatgttcc actctatgga
     3061 aacaccgttg ggcgcagttt tgcgttccgc ggtgtttttt cgcaaggaag gtaatcatca
     3121 gtaacgcaca tattg
//
LOCUS       DASTJP010000331        12018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1049257, whole genome shotgun sequence.
ACCESSION   DASTJP010000331 DASTJP010000000
VERSION     DASTJP010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049257"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFQ23_09930"
     CDS             <1..803
                     /locus_tag="VFQ23_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906952.1"
                     /translation="NGKGILPVPLEPLADPNEYGDDRLFVYLRQTGDLDESMKALRAA
                     GHPVIDLSIPDFYDVGAEMYRWEIATAIACSVVGVNAFDQPNVETSKKLTKAKIAEYQ
                     ANGELKEGTPSWKQDGVAVFPSVEISGTDLKTILEDYLKNAKPGGYVAINAYLPRNTE
                     MTEALQELRVAIRAKTGNAVTAGFGPRFQHSTGQFHKGGPENALFIQITADPEKDIDI
                     PNQGLSFGTLIRAQALGDFEALIEAQRIALRVHLPNPQAVKALVKALQ"
     gene            883..1746
                     /locus_tag="VFQ23_09935"
     CDS             883..1746
                     /locus_tag="VFQ23_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9906953.1"
                     /translation="MRNLSKFIPLLLIALLLVILFKFRSEILLRESVVVVGAGDISSC
                     DNDNDESTAKLLDNIPGTVFVLGDNVYVDATYSDFTNCYHPTWGRHKDRTKPVPGNHE
                     YFTTDAAGYFQYFENVPSYYAYDLGDWRVYALNSEIDVTSTGEQIQWLKNDLAVNPRL
                     CVLAYWHTPLWSTRYEDGSDDTYETFWRTLHEAGAELVINGHVHNYERFSEMNANGEA
                     TSPGLREIVVGTGGVNHDRYVDASLSTSEVYNSSTYGVLKLILSPTNYSWEFVPVAGQ
                     TFEDRGRTDCH"
     gene            1989..2327
                     /locus_tag="VFQ23_09940"
     CDS             1989..2327
                     /locus_tag="VFQ23_09940"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET9906954.1"
                     /translation="MSDLRQMKCVACRAGEPTVTESEIEMLRPQIPEWQVKEVDGVKR
                     LERVFKFKNFVQALEFTNRVGAIAEEEDHHPLIITEWGKVTLQFWTHTIKGLHKNDFI
                     MAAKSDELYL"
     gene            complement(2367..3563)
                     /locus_tag="VFQ23_09945"
     CDS             complement(2367..3563)
                     /locus_tag="VFQ23_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9906955.1"
                     /translation="MTQTVLCLASYFKGSAFLQAAKEAGMQVILLTNDKFKDDDWPRE
                     SIDEFFNMPDLSKRPDIIHAVSYLARERKLDLIVALDDYDVETAAHLREHFRMPGMGD
                     TLARNFRDKLAMRTTARNAGLLVPEFTGVFNYDRLREFMDRVAPPWLLKPRSEASSMG
                     IKKINNAEELWPILDSLGDQGSFFVMEQFVPGDVYHVDGLVDNSEVLFSVAHKYAQPP
                     LSVYAGGGVFITRSLDRESDESKSLVDINKQVLKAMGIVRGATHSEYIRAHSDGQLYF
                     LENAARVGGANIAECVEYASGINLWREWAHIDIANLRGEQYKLPESHSGYAGVINCLA
                     RQDWPDLSGYNDPEIKWRLNKKYHAGLIVSSPDAARIESLLNNYAERFAQDFLAVMPP
                     RQSAAE"
     gene            complement(3583..4695)
                     /locus_tag="VFQ23_09950"
     CDS             complement(3583..4695)
                     /locus_tag="VFQ23_09950"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HET9906956.1"
                     /translation="MPEKPSFSIGIEEEYQIIDPQTRELKSYITEILEAGKMTLREQV
                     KAELHQSIVEVGTKVCSTPVEARDELVRLRRGVMELAGRNGLKIAAAGTHPFSSWVDQ
                     EITPFERYAGVKQDMQILAQQLLIFGTHIHIGIEDPEFLIDAMNVIRYLLPHVLCLST
                     SSPFWMGHETGLKSYRSVVFRNFPRTGIPPHFNSYLDFVQYRDILVNTRCIPDGTKIW
                     FDARPNHNYPTLEFRICDVFTRVDEAICAAAIFQALIYKLWKLRRDNMTFRMYSPSLI
                     EENKWRAMRYGLDGKLIDFGKQEELPTRELIREMIEWFIGDEMDELGSRKEIEYAYRI
                     LDEGTSADRQLATYRRTGNLTSVVDQLIAETQEGVV"
     gene            complement(4723..6075)
                     /locus_tag="VFQ23_09955"
     CDS             complement(4723..6075)
                     /locus_tag="VFQ23_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906957.1"
                     /translation="MNDPREPIDHYHSLLDVETARETHEQLTEQLRRRGLFFGERPLS
                     TVLRPRFIHPDQYRALQVAVREVMPAFQKAYVAALADSNFRSQFRLNDWEEELIQFDH
                     GFETPSPTARMDTFFDEAGMLWLTEYNAETPAAVAYNDALSEVYYGLPVIREFSKQYE
                     LRPLVGRHLMMHALIDCYKQWGGKEQPRIAILDWKEVPTYSEFVLFHNYFESHGYDCV
                     IADPREVTYENGKLIADGKPVHIIYKRVLISELVERGGLDHPVVRAVLDRAVCMVNPF
                     RCKILHKKASLAVLSDEANHHLFTAAERDAIQKYIPWTYLVEDRKIHHQGKEIDLLSF
                     LADNKDDYVIKPNDEYGGKGVVLGWETDSSEWEAALKTAINEPSIVQKKVPLPTFPYA
                     SWVEDHVEIYDRMIDTNPYIWYGSYVDGCLTRLSTEALLNVTAGGGSTVPTFIVDKRY
                     "
     gene            complement(6077..7003)
                     /locus_tag="VFQ23_09960"
     CDS             complement(6077..7003)
                     /locus_tag="VFQ23_09960"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906958.1"
                     /translation="MQKIGVIVGREWSWPPAFIEEVNDRNEDVIAQYAKLGGTRMDEV
                     SPYAVLIDRISHEVPYYRSYLKNAMLQGSYVVNNPFMWTADDKFFGAALITKLGVASP
                     KTVALPNKEYVPGIVHNESLRNLTFPIPWEEHIEYLGGFPVVLKDAHGGGWKQVHICR
                     SYQDLWDAYNQSGLLTMVLQEFIKWDEGQYLRCMCIGQSEIKVMKYDPDNRRYLSPEL
                     PADLHERVVSDSLKIVRAFGYDMNTIEWAIRDGIPYAIDFMNPAPDMDINTTGVEYHR
                     WCVEKMADMCIRLAKNPRPQVADVKWSTMFNG"
     gene            complement(7016..7747)
                     /locus_tag="VFQ23_09965"
     CDS             complement(7016..7747)
                     /locus_tag="VFQ23_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9906959.1"
                     /translation="MQRFMDGWHSPSLNKHMEIVTYGEHGFPLLLFPTAAADYLEYER
                     FYVIEVIREFIEAGKLKVFSINSINREGWMAKWMNPRDRVIRQVQYNQYVTDEVVPYI
                     WNHLEEQIGIITCGASLGAYHAANQLFRRPDLFDGMVAMSGNYNIRHFADGYYDDDLY
                     FNDPLAYLPNLADDHYLPMLQQKTQIHILSGQGAYENPDGSRQLAGILTSKGISYNLD
                     LWGYDMSHDWPTWRAMMKYYIGEKF"
     gene            complement(7750..8469)
                     /locus_tag="VFQ23_09970"
     CDS             complement(7750..8469)
                     /locus_tag="VFQ23_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9906960.1"
                     /translation="MNREHHRWYSPSLNRNMELLVFGHGGARMLAFPTSMGKFYEWED
                     RGMISALGEHLERGWIQLYCVDSMDAESWYAFWKEPGARAWRHIEYENYLLHEVLPLI
                     YAKNPTPYMIVAGASFGAYHAMNFALRHPDKTNRVIAMSGLYNIERWLGGYRDDNSYY
                     NCPCLYIPNESDPGRLELLRRLDIIMAIGREDPNISNNQWFSGALWGKGIGNALREWD
                     GWAHDWPWWKQMITKYVGGHD"
     gene            complement(8504..10165)
                     /locus_tag="VFQ23_09975"
     CDS             complement(8504..10165)
                     /locus_tag="VFQ23_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9906961.1"
                     /translation="MSLTATCPLCQRPIDPILSALQPKLENHIARILRENNPDLKRNS
                     VVCRECAHSAVEKAIEARSPTSLHAELLSPFPVYSRDEKQLLPTPIRVQANPNFTGRG
                     VTVAFLDSGFYPHPDLVRPRNRIVCYVDATRQTPVEKQKFKQPSVSSWHGLMTSSIFA
                     GNGFMSNHLYRGIAPDAQLVVVKTGNARKRGIRDRDISRALTWVLNNQERFNIRVVNI
                     SLGGDFPTTGKFTELDELVEEAVSRGIVIVAAAGNSGRNIIIPPASAPSAITVGGLQD
                     QNHLDPRYRRLYWSSYGRSINGVRKPDIIAPAIWLAAPMLPKTRVHNTSLYLSRLSRA
                     SDAQLSKLLKSSYAKTRLGKKILSVPLAEARIAIQELMNEQKFIHPHYQHVDGTSMAA
                     PIVSGIIAQMLEANPSLTPPQIKEILMATADPLNDAPAERQGAGAVNAGRAVAAALRF
                     KGGLLQGIPISPHVAPNDPTFYYYDPKAREVMLVGSFNEWQSEGSAMVQIRPGLWEGV
                     LDVSERGIHRYKFLIDRSRLINDPENPLRIEDGFGGFNSLITIDK"
     gene            10253..11125
                     /locus_tag="VFQ23_09980"
     CDS             10253..11125
                     /locus_tag="VFQ23_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9906962.1"
                     /translation="MNNKFTSPDFLKFIKRVAVDLIVLSVGGANGGLFVQPAHLKVEN
                     VSLRLRRLTPAFHGIRIAQISDIHMGGWMNRERFQQIAELVSSLQPDLLCLTGDFLVG
                     STFNKRSEEDLHSLVTILAPLTKSIPTFAVLGNHDYWTNVTAVREMLRASGCIELTNS
                     VYTLTRGNEYLKICGVDDIWEGRVRLREVLRQLDDDSPAILLAHEPDFADQSAMSGRF
                     DLQISGHSHGGQLVIPYFGSPILPYLGRKYPSGLYKIGDMYQYTNRGVGTGRLPVRLN
                     CPPEITIFTLETLS"
     gene            complement(11147..>12018)
                     /locus_tag="VFQ23_09985"
     CDS             complement(11147..>12018)
                     /locus_tag="VFQ23_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906963.1"
                     /translation="IPVGITWEGVPYALDVSHPEVLAWLDALIRKVRSWGYDYLKLDF
                     LYAGALPGKRYADIPREVAYRNAFQVIREAAGDAYILACGAPILPSIGLCDGIRIGPD
                     VSPFWLNTPLTVWLNNPNDTSTQNAIRTSIHRLWMKPFVNVDPDVMFFRSRHNALKSN
                     EQQLLIDLGNLTGFKATSDLPQWMRPREIEALREFLESEVEIKQLNRYQYEVSGRGVD
                     FNPAVPLHMHRNFPVWFAQNLGLLKIARRQVLPAVLESLKHYHELRWLFYPLATSLSL
                     FTAIGSHSDATTL"
BASE COUNT         2901 a         2985 c         3085 g         3047 t
ORIGIN      
        1 agaacgggaa ggggattctg ccggtgccgt tggaaccgtt ggctgatccg aatgaatacg
       61 gtgacgatcg attgttcgtt tatttgcgtc aaacgggtga tctcgacgaa agcatgaaag
      121 ccctgcgtgc cgctggtcat cccgtgattg atctatcgat tcctgatttt tacgacgttg
      181 gcgcggagat gtatcgttgg gaaatcgcga ctgcaattgc ctgttcggtt gtgggtgtga
      241 atgcatttga tcagccgaat gtggaaacca gtaagaagct tacaaaggcg aagattgcgg
      301 agtatcaggc aaacggggag ctaaaagaag gaacgccctc ctggaagcaa gatggcgtgg
      361 cggtatttcc atcggtggaa ataagcggca ccgacctgaa gacaatcctt gaggactacc
      421 tgaaaaatgc caagcctggt ggatatgtag ccatcaacgc ttatctgccg cgcaacaccg
      481 agatgaccga agccttacaa gagctgcgtg tggcgatccg ggcgaagaca ggcaatgccg
      541 tgacggcggg attcggtccg cgctttcagc attcgaccgg gcaattccac aaaggcggac
      601 ccgagaacgc gttgttcatt caaatcacgg ctgaccccga aaaggatatt gacattccaa
      661 accagggtct cagctttggc acgttgatcc gtgcgcaggc cctgggtgat tttgaagcgc
      721 tcattgaagc acagcgcatc gccttgcgag tgcatttgcc aaaccctcaa gctgtgaagg
      781 cattggtaaa ggcattacaa tgaccaagcc atcacaacaa aatgcaccgt cagtgagtga
      841 gggttagaca gttaacttgc cttgctttgt tcatgcgcac gcatgagaaa tttaagcaaa
      901 tttatcccac tgctacttat agctttgctt ttagttatct tatttaagtt cagatccgaa
      961 atactgcttc gcgaatccgt tgtcgtagtg ggtgcgggag atatctcctc gtgcgataac
     1021 gacaacgatg aatcgactgc aaaattattg gataacatcc caggcaccgt atttgttctc
     1081 ggtgataatg tgtatgtcga cgcaacttat agtgacttca caaactgtta ccatcccacc
     1141 tggggacggc ataaagacag gaccaagcct gtaccgggaa atcatgaata tttcacgacg
     1201 gatgccgcag gctacttcca atatttcgag aatgtccctt cgtattacgc ctatgacctg
     1261 ggggattggc gcgtttacgc cctaaactct gagattgatg ttacctctac cggtgagcaa
     1321 attcaatggc tgaaaaatga tctcgccgta aaccccaggc tttgtgttct ggcctactgg
     1381 catacaccgc tctggtcaac aaggtacgaa gacggcagtg atgatactta tgaaaccttc
     1441 tggagaacac tccatgaggc aggcgcagag ctggtgatca atggtcatgt gcataattat
     1501 gagcggttca gcgagatgaa tgcgaacgga gaagctactt cccccggttt gcgtgaaatt
     1561 gtagttggaa cgggaggagt gaaccatgat cgttacgtgg acgcgagctt atctacaagt
     1621 gaggtctata actcctccac gtatggtgtc ctgaagttga ttctaagtcc tacaaactat
     1681 tcatgggagt ttgttccggt cgctgggcag acctttgagg accgtggacg cacggattgt
     1741 cattgatggg cgcaagttgt gaaggatgca aggagtataa tttcagtaaa ttacagatgt
     1801 catatgtcat tgcgaggagg gttgagatgc gacagcatcg aaagcccgac gaaagaacct
     1861 tctggttcac atattcactt tcatagccag tttacaccgt ttaagttgag attgtttcgt
     1921 cgcacacctg ccctagcggg cggtgccagt gacaactgct cctcgcaatg acaatcaaag
     1981 gaattaagat gtcagattta cgtcaaatga aatgcgtcgc ttgccgcgct ggcgaaccaa
     2041 ccgtgacgga gagtgaaatc gaaatgcttc gtcctcagat tcctgagtgg caggtcaaag
     2101 aagtggatgg ggtgaaacga ttagagcgcg tcttcaaatt caagaatttt gttcaagcct
     2161 tggaatttac gaatagagtc ggggccattg ctgaagagga agatcatcat ccgctcatta
     2221 tcaccgagtg ggggaaagta acacttcaat tttggactca tacgatcaaa ggcttgcata
     2281 aaaacgattt catcatggcg gccaagagcg acgagctgta cctctaaagg tccattcctg
     2341 tagagttttg agaatcaggc gaatacttac tctgctgcac tttgcctggg tggcatcaca
     2401 gccagaaagt cctgtgcaaa tcgttcggcg taattattca acaaggactc tatcctcgcc
     2461 gcatccggag aagacacgat cagccctgcg tgatattttt tgttcaaccg ccattttatt
     2521 tccgggtcgt tatagcctga aagatcgggc caatcctggc gcgccaggca attgatgacg
     2581 cctgcatagc cggagtgcga ctctggcaat ttgtattgct cacctcgcag gttggcaatg
     2641 tcgatgtgtg cccactctcg ccacagattg atgcccgatg catattccac gcattcagcg
     2701 atattagcac cacccacccg cgcggcattt tccaaaaagt ataactgtcc atccgaatgc
     2761 gctctaatat attcagagtg agtcgcgccg cgcacgattc ccatggcctt tagtacctgc
     2821 ttgttaatat caaccagcga ctttgactca tccgactctc gatccagcga gcgcgtgatg
     2881 aacacgcctc cacctgcata tacggaaagt ggcggctggg catatttatg tgctaccgaa
     2941 aataatactt cgctgttatc tacgaggccg tccacgtgat agacatctcc aggcacaaac
     3001 tgttccatca cgaagaacga tccctgatcg cccagcgagt ctaaaattgg ccacaattcc
     3061 tctgcattgt tgattttctt gatgcccatc gagctggctt cagagcgcgg cttgagcagc
     3121 catggtggtg cgacccgatc catgaactcg cgcaaacggt catagttgaa aacacccgta
     3181 aactcaggga ccagaagccc tgcattgcgc gcggtcgttc gcatggctag tttgtcgcgg
     3241 aaattgcgag ccagcgtgtc tcccatgcct ggcatgcgaa agtgttcccg caggtgggca
     3301 gctgtttcca catcgtaatc atccagggcg acgatcagat ccagtttccg ttcgcgtgcc
     3361 agataactta ccgcatggat gatatctggc cgtttactga gatcaggcat attgaagaat
     3421 tcatcaatgc tttcgcgcgg ccagtcatca tctttaaact tgtcattggt gagcaggatg
     3481 acctgcattc ccgcttcctt cgcagcctgt aaaaacgcgc tgcctttgaa gtagctggca
     3541 aggcagagga cggtctgtgt catacgtttc tcctgatacg ggtcaaacca caccttcctg
     3601 tgtttctgca atcagttggt ccacaacgga ggtcagattc ccagttcgcc ggtatgtggc
     3661 aagctgacgg tctgcactgg tgccttcgtc cagaatgcga tatgcgtatt cgatctcttt
     3721 tcggctgccg agctcgtcca tttcatcacc tataaaccat tcaatcattt cgcggatcaa
     3781 ttcacgcgtt ggcagttctt cctgtttgcc gaaatcgatg agcttcccat ccaatccgta
     3841 acgcatggct ctccacttat tctcctcgat cagagatggc gaatacatcc ggaaggtcat
     3901 attgtcgcgg cgaagcttcc aaagtttata gatcagcgcc tggaagattg ctgccgcaca
     3961 aatcgcttcg tctacgcgag tgaaaacatc acaaatgcgg aactccagag tcggataatt
     4021 gtgattggga cgcgcgtcaa accagatctt ggtgccatcc gggatgcacc tggtgttgac
     4081 caatatatcg cggtattgaa caaaatccag gtatgaattg aagtggggag gaattccggt
     4141 gcgagggaag ttgcggaaaa caacgctacg ataggattta agaccggtct cgtgtcccat
     4201 ccagaacgga gaactggtgg acaggcacaa gacatgcggc aagaggtacc gaataacgtt
     4261 catcgcatcg atcaaaaact ccgggtcttc gatgccgatg tgaatgtgag tgccaaagat
     4321 gagtagttgc tgcgccagga tttgcatatc ctgttttaca ccggcataac gctcgaaggg
     4381 tgtgatctcc tgatctaccc acgatgagaa cggatgggtt ccagcagcgg caatcttcaa
     4441 cccgttgcga ccggccagtt ccattacacc tcgccgcagc cgaaccagct catctcgcgc
     4501 ttcgacaggt gtgctgcaca ccttcgttcc aacttcgaca atcgattgat gaagttcggc
     4561 ttttacctgc tcgcgcaacg tcatcttacc cgcttcgagg atctctgtaa tgtaggactt
     4621 caactccctt gtttgtgggt caatgatttg atactcttct tcgattccaa tactgaaaga
     4681 aggtttttca ggcatagatt actcccagaa aagcgatttg tattagtatc tcttatccac
     4741 aataaaagtg ggaaccgtgg aaccaccgcc tgccgtgaca ttaagcaagg cctctgtcga
     4801 gagacgggtt aagcagccat ccacatagct tccataccag atgtacggat ttgtatcgat
     4861 catccgatca tagatttcga catgatcctc cacccaactt gcgtaaggga atgtcggcaa
     4921 cggaaccttt ttctgcacga tcgaaggttc gttaatagcg gtcttaagag cggcttccca
     4981 ttcgctcgaa tctgtttccc atcctaacac tacacctttg ccgccatatt cgtcattggg
     5041 tttgatgaca taatcatctt tgttgtctgc caggaacgac agaaggtcaa tctctttgcc
     5101 ttgatgatgt atcttgcgat cctcaaccag gtaggtccaa ggaatatatt tttggatcgc
     5161 atcgcgttct gcggcggtga aaaggtgatg attggcttca tcactgagaa cggccaggct
     5221 cgctttttta tgcagaatct tacatcgaaa tgggttgacc atgcaaacgg ctcggtccag
     5281 gactgcgcgc accacgggat gatccagccc gccgcgctcg actaattcac tgatcagcac
     5341 ccgtttgtag atgatatgaa ccggtttgcc atctgcgatg agtttgccat tttcgtaggt
     5401 tacttcgcgt gggtccgcga tgacgcagtc atagccgtgc gattcgaagt agttatggaa
     5461 gaggacgaat tcactatagg tgggaacctc tttccagtcg aggatcgcaa tgcgcggctg
     5521 ttcctttcca ccccattgtt tatagcaatc gatcagcgcg tgcatcatca aatgccgccc
     5581 gaccaatgga cgcagctcgt attgtttgct gaattcacgg atgacaggca gcccgtaata
     5641 cacttcggaa agggcatcat tgtaagcgac cgcggccggt gtctcggcat tgtattccgt
     5701 gagccagagc atgcccgctt catcaaaaaa agtgtccatg cgcgcggttg ggctgggagt
     5761 ttcaaatccg tgatcgaatt gtatgagctc ttcttcccaa tcgttcaaac ggaactgtga
     5821 acgaaagttt gagtctgcca gcgcggctac ataggccttt tgaaatgcag gcatcacctc
     5881 acggaccgcg acttgcagcg ccctatattg atccggatga atgaagcgtg ggcgcaggac
     5941 tgtgctcagc ggccgctctc caaaaaataa gccgcgcctc cttaactgct ctgtgagttg
     6001 ctcgtgggtt tcccgcgcgg tctcaacatc cagcagagag tgatagtgat cgataggttc
     6061 gcgagggtcg ttcatgctat ccgttaaaca tcgtggacca ctttacgtct gcgacctgtg
     6121 gccttggatt cttggctaga cggatacaca tatctgccat tttctccaca caccagcgat
     6181 gatattcaac gccggtggtg ttgatatcca tatccggcgc aggattcatg aagtcgatcg
     6241 cataggggat gccatcccgg atggcccact cgattgtgtt catatcgtag ccaaaggcgc
     6301 gtacaatttt cagtgagtca ctcacgacgc gttcatgcag gtcagcgggc agttctggcg
     6361 ataagtaacg gcgattatcg ggatcatact tcattacttt gatttcagat tgcccgatgc
     6421 acatacagcg cagatactga ccttcgtccc atttgatgaa ctcctgcaaa accatggtga
     6481 gcagcccgga ttggttgtac gcatcccaca aatcctggta ggagcggcag atgtggacct
     6541 gtttccagcc gccgccatgc gcatctttca gcacgaccgg gaatccccca agatattcaa
     6601 tgtgctcttc ccatggaatt ggaaaggtta ggttacgcag gctttcattg tgaacgatcc
     6661 caggaacata ttccttgttc gggagcgcca cagttttggg acttgcgacg ccaagttttg
     6721 tgatgagtgc cgctccaaag aatttatcgt cggctgtcca cataaagggg ttgttgacga
     6781 cataggatcc ttgcagcatc gcgttcttga ggtacgaacg ataataaggt acttcatgcg
     6841 agatgcggtc gatgaggacg gcatacgggg agacttcatc cattcgggtg ccgcccagtt
     6901 tggcatactg ggcaatcacg tcttcattcc gatcgtttac ctcttcaata aacgctggcg
     6961 gccaagacca ctcacgcccg acgattacac caattttttg catgattcct acctcctaaa
     7021 acttctctcc aatataatat ttcatcatgg ctcgccacgt tggccaatcg tgtgacatat
     7081 cataacccca taaatcaaga ttatacgaga tgcccttgga tgtcagaatt ccggctagct
     7141 ggcgtgagcc atccgggttt tcataggcgc cttgtccaga aagaatgtga atttgcgttt
     7201 tctgttgaag catcggcagg taatggtcgt cggctaagtt tggcaggtaa gcaagcggat
     7261 cattgaagta cagatcgtca tcgtaatagc cgtcagcaaa atgtcggata ttgtagttgc
     7321 cgctcatggc gaccattcca tcgaacaagt ccggacggcg gaaaagttga ttggccgcat
     7381 gataagctcc cagactcgcc ccgcaggtga taatgccaat ttgctcctct agatgattcc
     7441 aaatatacgg aaccacttcg tcagttacat attgattata ctgaacctgc cggatgacgc
     7501 ggtctcgcgg attcatccat tttgccatcc agccttcgcg gttgatgcta ttgatggaaa
     7561 agactttgag cttgccggct tcgataaatt ctctaataac ttcgataacg taaaagcgtt
     7621 cgtactccag atagtcagcc gccgcagtgg ggaataagag cagagggaaa ccatgttcac
     7681 catatgtgac gatctccatg tgcttgttga ggctggggct gtgccagcca tccatgaagc
     7741 gctgcatggt caatcgtgtc cgccgacgta cttagtaatc atttgcttcc accacggcca
     7801 atcatgtgcc cagccatccc attcacgcag ggcattgcca atccctttgc cccacagcgc
     7861 gcctgaaaac cattggttat tactaatgtt gggatcctcg cgcccaattg ccataatgat
     7921 gtcgagtctt cgtaagagct caaggcgacc cggatcagat tcatttggaa tatatagaca
     7981 cgggcaattg taatagctgt tgtcatcacg gtagcctccg agccagcgct caatgttgta
     8041 gagtccgctc atggcgatga cacggttggt tttatccggg tggcgcagtg caaagttcat
     8101 tgcatggtat gcgccaaagc ttgcccccgc aacgatcata tatggagttg gattcttagc
     8161 gtagatgagc ggcagaactt catgaagtag atagttctcg tattcaatat gtcgccaggc
     8221 tcgcgccccg ggttccttcc aaaatgcata ccagctttct gcgtccatac tatccacgca
     8281 ataaagctga atccaaccgc gttccaaatg ttcccctaat gcgctgatca tgccgcgatc
     8341 ttcccattca tagaacttgc ccatggatgt tggaaaggcc aacatgcgtg caccgccatg
     8401 cccaaaaacc agcaactcca tgttgcgatt gagcgatggg ctataccagc gatgatgttc
     8461 tcggttcata agaattgatg gatgggaata agcagattgg atgtcattta tctatggtta
     8521 ttagcgaatt aaacccgccg aatccgtctt cgatacgcaa gggattttca gggtcattga
     8581 ttaaacgcga acgatcgatc agaaatttat agcgatgtat tccacgttct gatacatcca
     8641 agacaccttc ccataggcca ggcctgatct gaaccatggc actgccctcc gactgccatt
     8701 cgttgaaact gcccacgagc atcacctctc gagccttggg atcgtaatag tagaatgtgg
     8761 ggtcgttcgg ggcaacatgt ggcgaaatcg ggattccctg cagcaaacca cctttgaagc
     8821 gtaaggcagc cgcgacggct cgccccgcat tgactgcacc ggcaccttgt ctctctgctg
     8881 gggcgtcatt caatggatca gccgttgcca ttaaaatctc cttgatctgg gggggcgtga
     8941 gtgacgggtt tgcttcgagc atctgggcga ttattccgct tacgatcggg gcagccatgg
     9001 aagtgccatc cacatgctgg taatgtggat ggatgaattt ctgctcattc atcaattcct
     9061 ggattgcgat acgagcctcg gcaagcggca cacttaatat ttttttaccg agccgcgttt
     9121 ttgcatagct cgattttagc agcttgctga gttgtgcgtc actggcgcgt gacagtcgcg
     9181 aaagataaag ggatgtgttg tgcacgcgcg ttttcgggag catgggtgcg gccaaccaaa
     9241 ttgccggtgc aataatatca ggcttgcgta ccccgttgat gctacggccg taacttgacc
     9301 aatacaagcg gcggtagcgc gggtcgagat gattctgatc ctgcaaaccg ccgaccgtaa
     9361 tcgccgaggg agcactggcc ggtgggataa tgatgttacg tccgctattg ccagccgctg
     9421 ccacgatgac gatcccacgc gataccgcct cttcgaccag ttcgtccaac tcggtgaatt
     9481 ttccggtcgt cgggaaatcg ccgcccagcg agatattgac cacacggatg ttgaatcgtt
     9541 cctggttgtt caacacccag gtcaacgctc ggctgatgtc gcgatcgcgg attccacgtt
     9601 tgcgtgcgtt gccggttttc accaccacaa gctgggcatc cggggcgatc ccgcgataca
     9661 ggtggttgga catgaaaccg ttccctgcga aaatggagga ggtcatgagt ccatgccagc
     9721 ttgagacact cggctgtttg aatttttgtt tctctacagg ggtttgcctt gtggcatcta
     9781 catagcacac gatgcgattt ctgggtcgca caaggtcagg atgtggatag aagcctgagt
     9841 ccagaaaggc gacagtcaca ccacgaccgg tgaaattcgg gttggcttgt acacgtatgg
     9901 gtgtgggcag taactgcttt tcatccctcg aatacacagg aaacggactg agcaactccg
     9961 cgtggagtga ggttggactg cgcgcttcaa tggctttttc aactgcgctg tgagcacatt
    10021 cccggcagac gacgctgttg cgcttcaaat cagggttgtt ctcgcgcaaa atgcgagcaa
    10081 tatgattttc aagcttgggt tgtagtgcag aaaggatggg gtctatgggg cgttggcaga
    10141 gagggcaggt ggcggtgaga gacattgatg agttcttgat cgtccgcagt taagatgtgc
    10201 aaattgtact actttatttt ggaggatggt gcgttatctt ataattctgc agatgaataa
    10261 taaatttaca agccctgatt ttctaaaatt tataaagcgg gttgccgttg acctgatcgt
    10321 attatccgtc ggcggtgcca acggcgggct ttttgttcag ccggcacatc tgaaagtgga
    10381 aaacgtatcc ttgaggttgc gacggctcac cccggctttt cacggcatac gcatcgcgca
    10441 aataagtgat atccacatgg gaggctggat gaatcgggaa cgctttcagc agattgccga
    10501 gctggtgagc tcgcttcaac ccgatctatt atgtttgacc ggagattttc ttgtcggctc
    10561 aacgttcaat aagcgttctg aagaagacct tcacagcctg gtcacaattc tggctcctct
    10621 gacgaagtcc atccccactt ttgccgtgct tggaaaccac gattattgga caaatgtaac
    10681 tgcggttcgt gaaatgttaa gagcaagtgg atgcatcgaa ttgaccaatt cagtttatac
    10741 cttgacgcgt ggcaatgagt atctgaaaat ttgtggtgta gatgatatct gggagggaag
    10801 ggttcggttg agggaagtgc ttcgccaact ggatgacgat agccctgcga ttctgctggc
    10861 tcatgaaccg gattttgctg accaaagtgc gatgtccggt cgctttgact tgcagatctc
    10921 tggtcattcg catggcggtc agctcgtgat tccgtatttt ggttcgccaa tattgccata
    10981 tcttggacga aaatatccga gcggtctcta taagattggc gatatgtacc aatacacaaa
    11041 tcgcggagtg gggacgggca gactgcctgt gcgtttgaat tgcccacccg agattacaat
    11101 atttactttg gagactcttt cataagagcc tgacccaacg acttacttaa agcgtagtgg
    11161 catcggagtg gctgccgatt gctgtaaaca gcgatagact ggtcgcaagg ggatagaaaa
    11221 gccagcgcag ctcatgatag tgtttcaggc tttccagcac agctggaagg acctgtcgcc
    11281 tcgctatctt taacaacccc aggttttgtg caaaccaaac tggaaaattt cgatgcatgt
    11341 gtaagggaac tgcgggattg aagtccacgc ctcgtccgct cacctcatat tgatagcgat
    11401 tcaactgttt gatctctacc tcagattcca aaaattctcg tagtgcttcg atctcacgcg
    11461 gccgcatcca ttggggtaga tcggaggtag ctttgaagcc tgtcaggttt cctaaatcga
    11521 tgagcaattg ctgctcattt gacttgagcg cgttgtgtcg cgaacggaag aacatcacgt
    11581 ccgggtccac gttgacaaag ggcttcatcc agaggcggtg aatgctggtg cgaatcgcat
    11641 tttgggtgga tgtatcgttc gggttgttta accacaccgt caacggcgtg ttcagccaaa
    11701 acggagagac atctggtcca atgcgaatgc catcgcatag cccgatggac ggcaggatcg
    11761 gtgcgccaca ggcgaggatg taggcatcgc ctgccgcctc acgaatgact tgaaaagcgt
    11821 tccgatacgc tacttctcgc ggaatatccg catagcgctt gccgggcagt gcacccgcgt
    11881 agagaaaatc gagcttgaga tagtcgtacc cccaactgcg tactttgcga atcagtgcat
    11941 ccagccatgc caggacttca ggatgcgata catcaagagc gtaaggtacg ccttcccacg
    12001 taattccgac ggggatcg
//
LOCUS       DASTJP010000332         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_191199, whole genome shotgun sequence.
ACCESSION   DASTJP010000332 DASTJP010000000
VERSION     DASTJP010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191199"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1223)
                     /locus_tag="VFQ23_09990"
     CDS             complement(117..1223)
                     /locus_tag="VFQ23_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HET9906964.1"
                     /translation="MSTSAKSKLHQMTMEFPTEYWNDSCSEEELTYAISHGAVGATSN
                     PTIVHAVLKKEMHLWRDRIHTLIRENPVWSESEITWKLFEEIAVYGAGFLSPIFEKHK
                     GKKGRLSIQTNPTLYRNAEALTEQAIHFNNLAPNMIVKIPVTKAGVKAIEEVTYHGVS
                     INATVSFSVPQAIAVAEAVERGLNRRVAEGKSIDEMAPVCTIMVGRTDDWMKVVASRD
                     GIEIDPSYLDWAGIACMKKAYEIYQQRGYRARLLAAAYRHLGHWAEFIGGELVVSLPY
                     EWQLKANASDIEVKERMNIPVESKIVDEMYKEIPDFRRAYDEDGMSIDEFDEYGATVR
                     TLRTFITSAHDLMGEVREFMLPNPDIKKVESLEA"
     gene            complement(1239..2111)
                     /gene="iolB"
                     /locus_tag="VFQ23_09995"
     CDS             complement(1239..2111)
                     /gene="iolB"
                     /locus_tag="VFQ23_09995"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET9906965.1"
                     /translation="MQSAVHSLLVSPNFNQSEYIHVTPDSAGWDHLSFAARKMGLGDR
                     WDFETQNNELALVVLGGTCEISSNQGNWTDVGRRPNVFSGMPYTLLLPPQTRFTVSAQ
                     SEMLDIAYGWCTAKDAHPARLVKPAEVEIEIRGGANVTRQINKMIPPGFPASRLVVVE
                     VYTPSGNWSSYPPHKHDEHIVDAAGNVLEADLEEVYFYKIDRPEGFAYQRIYTPDRQI
                     DELILARDSCLVLSPQGYHPVVAAPGYNCYYLNMLAGSAQSLAATDDPEHTWVKETWK
                     EKDPRLPLVSMDME"
     gene            complement(2131..2946)
                     /locus_tag="VFQ23_10000"
     CDS             complement(2131..2946)
                     /locus_tag="VFQ23_10000"
                     /inference="COORDINATES: protein motif:HMM:NF004847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9906966.1"
                     /translation="MADNSIFNGKYSIVTGSTQGLGEAIARLFVERGAAGLIICGRNA
                     ERGNAVAADLNSEACRVYFVQADLANVEDCRRIVAAADEHFGTVHSLVNAAGVSDRGT
                     IWDTTPELWDWIMAINVRAPFILIQESIKIMRRENVEGSIVNIGSVSAYGSVPFLTPY
                     ATSKGALMTLTKNAAYSVMRHRIRINMLNIGWMDTPGENVIQHKYHDANDHWLESAEQ
                     NMPFGRLLKPEEVARAVAFMASDESGMMTGSIVDFDQSVQGSGPQPIPPADAA"
     gene            complement(2936..>3121)
                     /locus_tag="VFQ23_10005"
     CDS             complement(2936..>3121)
                     /locus_tag="VFQ23_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906967.1"
                     /translation="PRHSKTYRNFRAEYDRLQQERISAFKELIADIQNGTYPESKHIV
                     PIKNDEFNAFMAVVDGR"
BASE COUNT          742 a          839 c          730 g          810 t
ORIGIN      
        1 gtttccttgc cgtcgcgatg gacatactga ttcttcagaa atttgatgat ggcttgtgca
       61 gtcgtcaatc gtaccgttgc catggatttc tcctcactat gaaagtgaaa ggcttgctac
      121 gcctctaaac tttcaacttt cttgatatcg gggttgggca acataaattc acgaacttca
      181 cccatcaggt catgggcgga ggtgatgaag gtgcgcaagg tccgtacggt cgcgccgtac
      241 tcgtcgaact cgtctatgct cataccatct tcgtcatagg cgcggcgaaa gtcgggaatt
      301 tctttataca tctcatccac gatttttgac tcgaccggaa tgttcatccg ctccttgact
      361 tcaatatcgc tggcattggc tttgagctgc cactcatacg gcaacgagac gaccaactcg
      421 ccgccgatga actcagccca gtgtcccaga tgtcgatagg ctgcagcaag caaacgcgcc
      481 cggtaaccgc gctgctgata aatttcatag gcttttttca tgcaggcaat gccggcccaa
      541 tccagatagg acggatcgat ctcaattcca tcacggcttg caacgacctt catccaatcg
      601 tcggtacgcc ccaccatgat ggtgcagaca ggcgccatct catcaataga cttgccctct
      661 gccacacgac ggttgagtcc gcgctcgaca gcttcagcga ctgcaatcgc ttgcggcaca
      721 ctgaaactga ccgtcgcatt gatcgagaca ccgtgataag tgacttcttc aatggccttg
      781 acccctgcct ttgtgaccgg aatctttacg atcatattcg gcgccagatt gttgaaatgg
      841 atagcctgtt ccgtcagcgc ctcggcattg cgatagagag tcggattggt ctgaatggag
      901 agccgcccct tcttcccttt atgtttttca aaaatgggag atagaaagcc tgcgccatac
      961 actgcaatct cttcgaacaa cttccacgta atctccgatt cagaccacac tggattttcc
     1021 cggatcagcg tgtggatacg atctcgccac aagtgcattt cttttttaag gaccgcatgc
     1081 acaattgtgg ggttcgatgt tgcaccaact gcgccatgac taatggcgta cgtcagttcc
     1141 tcttccgaac aggaatcatt ccaatattcg gtgggaaatt ccatcgtcat ttgatgtaat
     1201 ttgcttttcg cagatgtaga catgatcatc tccttcgtct attccatatc catggacaca
     1261 agcggcaggc gcggatcttt ttccttccag gtctccttga cccacgtatg ttcaggatca
     1321 tccgttgctg ccagtgattg tgccgagcca gccagcatgt tcaggtaata acagttgtag
     1381 ccgggcgcgg cgacaacggg gtgatatccc tgtggtgaaa gaacaagaca gctatcacgc
     1441 gccaggatca attcatcgat ttgtcggtcg ggggtgtaga tgcgctgata agcgaatccc
     1501 tccggacggt caatcttgta aaagtagacc tcttcgagat ctgcctccaa cacattcccc
     1561 gcggcatcta caatgtgttc atcatgcttg tgagggggat acgaactcca attgccagat
     1621 ggcgtatata cctccacgac aaccaggcgg ctggccggaa aacccggagg aatcatcttg
     1681 ttgatctgcc gggtcacgtt cgcgccgcca cggatctcga tctcgacctc cgccggcttc
     1741 accaaacgag cggggtgtgc atcttttgct gtacaccatc cataggcgat atctaacatt
     1801 tcactctgtg cactgaccgt gaatctagtt tgcggcggca gtaacaatgt gtatggcatc
     1861 ccggaaaaaa cattcgggcg tctacccacg tccgtccagt tgccctggtt tgatgaaatt
     1921 tcgcaggttc caccgagtac cactaaagca agttcattgt tttgcgtttc gaaatcccac
     1981 ctatctccaa gacccatctt acgcgcggca aatgatagat gatcccaccc agcagaatca
     2041 ggtgtcacgt gaatatattc actttgattg aagttgggag aaacaagtaa tgagtgaact
     2101 gcagattgca tcaggtctcc aatatcatga tcaagcggcg tctgctggcg gtatcggttg
     2161 tggaccgctg ccttgtacag attgatcgaa atcaacgatt gagccagtca tcatgccaga
     2221 ctcgtccgat gccataaatg cgacagcacg cgcgacttct tctggtttga ggagtctgcc
     2281 aaatggcata ttttgttctg cgctttcgag ccagtgatcg ttagcatcat ggtatttgtg
     2341 ctgaatgaca ttttcgccgg gcgtatccat ccagccaatg ttgagcatgt taattcgaat
     2401 gcgatgacgc atgacagaat acgctgcatt cttcgtgagc gtcatcaatg caccttttga
     2461 ggtggcataa ggcgtgagaa aagggacact cccgtaggcc gacacactac cgatattgac
     2521 aattgatccc tccacatttt cacggcgcat aatcttgatt gattcttgaa tcagaataaa
     2581 tggcgcgcgg acgttaatag ccataatcca atcccacaat tccggcgtag tatcccatat
     2641 tgtgcctcga tcagaaacac cggcggcgtt caccaggctg tgaaccgtac cgaaatgttc
     2701 atcagcagca gccacaatgc ggcggcagtc ttcgacattt gccaggtcag cctgcacaaa
     2761 ataaacccga catgcctctg agttcaagtc cgccgcgaca gcatttccgc gttccgcgtt
     2821 ccgcccacag atgatcagcc ccgctgcgcc gcgttctaca aataaacgcg caatcgcttc
     2881 gcccaatccc tgcgtgctgc cggtcacgat actatatttg ccattgaaaa tagagttatc
     2941 tgccatctac caccgccata aacgcattaa attcatcatt cttaatcggc acgatgtgtt
     3001 tcgattccgg atatgtgcca ttctgtatat ctgcaatcaa ttctttgaag gcagagatac
     3061 gttcctgttg taacctatcg tattctgctc tgaaattgcg gtaggttttg gagtgacgtg
     3121 g
//
LOCUS       DASTJP010000333         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_741896, whole genome shotgun sequence.
ACCESSION   DASTJP010000333 DASTJP010000000
VERSION     DASTJP010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741896"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFQ23_10010"
     CDS             complement(<1..358)
                     /locus_tag="VFQ23_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906968.1"
                     /translation="MYSKHNKIAVLILMIVILSACGGQALDSATPPSQPTEVVVPAIS
                     SPTGAETSTSLPVSTDTSIPASTDTAAPATEPPTATQPAVVDATVSFANDVLPIIQSR
                     CINCHGGDRTEEGLVLL"
     gene            complement(367..804)
                     /locus_tag="VFQ23_10015"
     CDS             complement(367..804)
                     /locus_tag="VFQ23_10015"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET9906969.1"
                     /translation="MELINRIINALTQLHPTHPMFVHFPIALTGAAFLFIVLAYWRKN
                     ASLEQTAFANMVLAAISTLAAGITGIMDNVKNYVGEAPNAGTKVILATVLFLLTTGLS
                     ISRFRNPNLFRTGNPILYIAAYGASFAIALVLAFLGGVILYGF"
     gene            complement(1079..1519)
                     /locus_tag="VFQ23_10020"
     CDS             complement(1079..1519)
                     /locus_tag="VFQ23_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906970.1"
                     /translation="MKRVFLILLSIVVVIGVLAGAGFAGYQIGLRHGARASSRDEIGP
                     LLARPNFGLERGPFAFERGFERDFDHGFPHGGFQRMRRGGFGFFGPFMFLGHILFWGL
                     ILLLAYWLFTRSGWRLTRTEQTVQSTSPNVQTVTTVQEEETRNE"
     gene            complement(1632..2993)
                     /locus_tag="VFQ23_10025"
     CDS             complement(1632..2993)
                     /locus_tag="VFQ23_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9906971.1"
                     /translation="MRSISSKLILAFLSIGLVSVLIIAVTAVWSTRTEFIRFLTDQTQ
                     TDIVTQLSDYHRSYGSWERVESIFVYNDHGQPGMGPGPQLRRQPFALTNANGRVIIPN
                     GPFRRGESLSAATLESGIPLMQDGELIGYLIPMRGQFEGAPRELEFIQRINRLLLYGA
                     FIGAVIALVLGVFLSRTLTRPIRELTQATHAVSEGDLSQQVPVRSNDELGELAQAFNR
                     MSSELSRSVNTRKQMTADIAHELRTPLSLILGHAEAVHDGVLPPTRENFEIIREEATR
                     LEHLVNDLRILSLADAGELTIHLQTIEPERLLQEVASLYQYETQRKNITLALDISSDL
                     PPIEVDPGRITQVLTNILDNALRHSPEGGQIALSAGQSNDWVELSVQDSGPGLRAEDV
                     DRIFDRFYRTDSARQRNDGGSGLGLAIARSIVQAHGGQLSAQSEEGKGLNVILKLPVK
                     SKR"
     gene            complement(2997..>3278)
                     /locus_tag="VFQ23_10030"
     CDS             complement(2997..>3278)
                     /locus_tag="VFQ23_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9906972.1"
                     /translation="ITVDRDSRTVRVAERFMDLTPSEFDILTALMTIPGRVYSRLDLL
                     DIIQGVRYEGYERTIDTHIKNLRGKIERDPRTPQYIETVYGVGYRFKRA"
BASE COUNT          839 a          814 c          857 g          768 t
ORIGIN      
        1 tcaaaagcac cagaccctct tctgttcgat cgccaccgtg gcaattaata cagcggctct
       61 gaataatcgg aagcacatca ttcgcaaagc tgaccgtcgc gtcaactacg gcaggttgtg
      121 tggcagtcgg aggttcagtt gctggggcag ccgtatcagt cgatgctggg atagaagtgt
      181 cggtggaaac cgggagactg gttgacgttt cggcccccgt tggggatgat atcgcgggca
      241 caacgacctc ggtcggttgt gaggggggag tagctgaatc cagtgcttgc ccaccacatg
      301 cagataggat gacgatcatc agaatcaaga ctgcaatttt attgtgtttt gaatacatgt
      361 attatcctaa aatccgtata agatgacgcc acccaaaaat gcaagtacca gggcaatcgc
      421 gaaactggca ccatacgcag cgatgtataa gatagggttg cctgtccgaa acaagttcgg
      481 gttgcgaaaa cgcgaaatac tgagcccggt cgtgagaaga aatagaactg ttgcgagaat
      541 gaccttcgtc cccgcatttg gcgcttcgcc aacgtaattt ttcacattat ccattatccc
      601 ggttattcct gctgcaagag tactaattgc tgcaaggacc atattggcga aggcggtctg
      661 ctcaagggat gcgttttttc tccaataagc caatacgatg aaaagaaacg ccgcgcctgt
      721 gagggcaatc gggaaatgta caaacatggg atgtgtagga tgaagctgtg taagtgcatt
      781 aatgattcga ttgattaatt ccatatttct ctccgtattc tcctctatat gagcctctaa
      841 aggttttcca gcaggctaag tgaaaaattg atcacatcag gtttacgata tattatctaa
      901 gaccctggga tcccagggtc ttatgtgtct tcaacttgaa agccagacgg aacagcctcg
      961 gcaatacgta ctcaatttta cgcgtgtcgc attaatagaa agtaaacttt tcaattgcac
     1021 gaggctgcaa tcaaaaccca aagcttacct gatgatgcgg cacgatgtaa ttgagaaatt
     1081 actcgtttct cgtttcctct tcctgaactg ttgtcacggt ttggacgttc ggtgaggtac
     1141 tttgaaccgt ttgttccgtc cgtgtgagcc tccatccgct gcgtgtgaac agccaatagg
     1201 ccagcaggag tatcaggccc cagaagagga tgtgacctaa aaacataaaa ggtccaaaga
     1261 aaccaaagcc accgcgccgc atcctctgaa aaccaccatg cggaaagccg tgatcaaagt
     1321 cgcgctcgaa gcctcgttca aaagcaaagg ggcctctctc caagccaaaa tttggacgag
     1381 caaggagagg tccgatctcg tcacgggagg aggcacgcgc gccatgacga agcccgattt
     1441 gatagccggc aaagcctgcg ccagccaata cgccaatcac taccacgata ctaagcagga
     1501 tgagaaatac tcttttcatt gttcatttcc tttcacatat tttctcaaat ttaaacatgc
     1561 aggggtaaag aactggtgtg aaaattgtgg ggaaattata gagatttggc tgatgaaatc
     1621 tacgacaaaa atcaccgttt ggatttcacc ggcagcttga ggatgacgtt caagcctttt
     1681 ccttcttcgc tttgtgcaga gagctgccca ccgtgcgcct gaacaatgga tctggcgatt
     1741 gccaacccca agcctgagcc accgtcattc cgctgccgcg cagagtcagt tcgataaaag
     1801 cgatcaaaga tacggtccac gtcttcggcc ctcaaccccg gcccactgtc ctgaactgaa
     1861 agctcaaccc aatcgttgct ttgacctgca gaaagcgcga tctgcccgcc ttcgggagag
     1921 tgccgtaggg cgttatccag gatgttcgtg aggacctgag ttattcgccc gggatcaact
     1981 tcaatcggag gaagatctga agagatatcc agtgccagcg taatgttctt tcgctgagtc
     2041 tcatactgat acagggacgc aacttcctga agtaaacgct ctggctcgat cgtctgtaga
     2101 tggatcgtca actcgccggc atctgccaga gagagaatgc gcaggtcatt cactaaatgt
     2161 tcgaggcgcg tggcttcttc gcgaatgatc tcgaagtttt cacgcgtcgg aggcagaacg
     2221 ccatcatgga ctgcttccgc atgcccaagg atcagactta acggcgtgcg cagttcgtga
     2281 gcgatgtctg ccgtcatttg cttgcgagtg ttgaccgaac gcgaaagctc tgaactcatc
     2341 ctgttgaatg cctgagcgag ttcacccagt tcatcgttgg agcgaaccgg cacctgctgc
     2401 gaaaggtcac cttctgaaac cgcgtgtgtg gcttgcgtaa gttcccgaat gggacgtgtc
     2461 agggtgcgtg aaagaaagac gcccaacact aacgcgatga cagcgccgat gaaggcgccg
     2521 taaagtaata gtcggttgat gcgctggata aattctaatt cgcgcggcgc accctcaaac
     2581 tgcccacgca tgggaataag atacccaatc agctcgccat cctgcatgag cggaataccg
     2641 gactctaatg tggcagccga aaggctttct ccacgacgaa aaggtccgtt gggaataatg
     2701 acgcgtccgt tggcgttggt aagggcgaac ggctgtcgcc tcaactgtgg gccaggaccc
     2761 atcccgggtt gcccatgatc gttgtaaacg aaaatggact ccactcgttc ccaggagcca
     2821 taactacgat ggtaatcgga aagctgcgtg acaatatccg tctgtgtttg gtcggtcagg
     2881 aatcgaatga actcggttct ggtgctccaa actgccgtga ccgcgatgat caaaacgctc
     2941 acaagcccaa tgctcagaaa agccagaatc agtttggatg agatggatcg catgcgttag
     3001 gcccgcttga agcgataacc aacaccatac acggtttcaa tatattgcgg ggtgcgcgga
     3061 tcgcgttcga ttttgcctcg caggtttttg atatgcgtgt cgatggtgcg ttcatagccc
     3121 tcatagcgta caccttgaat aatatccagc aggtcaagcc gtgagtagac gcggcccggg
     3181 atcgtcatga gggcggtgag gatgtcgaac tcggaggggg tgaggtccat aaatcgttct
     3241 gcgactcgta cggtacggct gtcgcgatct accgtgat
//
LOCUS       DASTJP010000334         6899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1009122, whole genome shotgun sequence.
ACCESSION   DASTJP010000334 DASTJP010000000
VERSION     DASTJP010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009122"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFQ23_10035"
     CDS             complement(<1..853)
                     /locus_tag="VFQ23_10035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9906973.1"
                     /translation="MQYSLLNKTIIITGGNSGIGKAAAVQLAKLGATVVLACRSKERG
                     LQALDEIQKAAQSTKVNLMQVDMSSQSSIRQFVADFEKRYERLNVLIHNAANFDHTQR
                     KPILSSDGLETVFATNHLGPFLMTYLFLDLLKASAPSRVITVASKGLITYPFLGIEFD
                     NLYGERKFSLQRAYYHSKQAQVMFTFDLAERLRGTGVTTHCVRVGNVAIPTERLEHLP
                     KWMLKLYELKRRFSMTPEKMAETYVWLAADPVGAQQTGGYWDAPGVPVKANSKAYNKE
                     TQASLWAI"
     gene            complement(974..1981)
                     /locus_tag="VFQ23_10040"
     CDS             complement(974..1981)
                     /locus_tag="VFQ23_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9906974.1"
                     /translation="MPLTIRDVAKKLDLSITTVSRALDGYDDVAEQTRELVIKTARKM
                     GYVPNRAARQLRRQKSETMGFIIPSISKRFDEAFFTEFISGLGEELSNQNFDLLIANA
                     TTDDGEHSMYKRWVSSRKVDGFILNRLHKQDWRVKYLTEENVPFVALGQSQEDLNYPC
                     IRVDGAEAYFNLVQHIKSRGFSRFAFLGGPKDLMDHVERLNWCKSALKKSGLEIERAN
                     IISTDMSSTAGYEATRRLFARSTPPDAILCVADEIAFGALHAAHEQGIRIGSEIAVGG
                     FDGVQDAKHSEPPLTTLDLPVFDIARQLVDMLLKTIAGTRPDSPVFIKPSLLIRPSTG
                     R"
     gene            2149..3060
                     /locus_tag="VFQ23_10045"
     CDS             2149..3060
                     /locus_tag="VFQ23_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906975.1"
                     /translation="MDFAIETNQLSKRYGDVLAVDSVNLRVKPGEIYGFLGLNGAGKT
                     TTIRGLLGMIRPSGGEIKILGQTIDSHGIGPWRRVGHLVESPSAYPELTVRENLEIAR
                     RLHEISDADAVSRVMERLTLTSYADRQAGTLSMGNLQRLGLARALLHEPELLILDEPA
                     NGLDPAGVLEIRTLLASLVNEKGVTIFMSSHILTEVDRLATRLGIIHQGRLIEELDTD
                     KLQKLRARWLEVRARDLEAAQFGLLSAGYTLTIKDDIIKIDDDYVIEHPDEIARILVN
                     AGAPPTHLAVEQQNLEEHFMQIIGGAK"
     gene            3057..3845
                     /locus_tag="VFQ23_10050"
     CDS             3057..3845
                     /locus_tag="VFQ23_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906976.1"
                     /translation="MNGLVASLWAETLKARRSKVPLLASIGFTLAPLMDGLFMFIMKD
                     PDRAREMGLLSVKAQLAISTADWATFFSVLTQAIAVGGVIVFSIVTAWVYGREFSDHT
                     AKDLMALPTSREKIVTAKLIVISGWVVITTVWVYLMGLIVGNFVDIPGWTSQLALSSF
                     LDVLVTAAMTLLLMTPVAFIASMGRGYLPPLGWAVLTIFFAQIVAATGWGAWFPWSVP
                     ALYSGVVGPREEQLGAHSYVLVILTSLIGMAATFWWWRNADQTR"
     gene            complement(4041..4478)
                     /locus_tag="VFQ23_10055"
     CDS             complement(4041..4478)
                     /locus_tag="VFQ23_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9906977.1"
                     /translation="MNTDPVRKWMTGRLMTITPQTTLPEAQRIMFDCKLRRLPVMEKD
                     KLVGIVTLGDLREAKASDATSLSIYEINYLIDRLTAKDIMTPNPITIAPDATIAEAAR
                     LMFEHKVGGLPVVDNGRLIGIITETDFCRLLMMQPEQFSENMP"
     gene            4754..>6899
                     /locus_tag="VFQ23_10060"
     CDS             4754..>6899
                     /locus_tag="VFQ23_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9906978.1"
                     /translation="MTEAPSGTVSFLFTDIEGSTKLSQEYPEEMPALLARHHEILNES
                     IEAHHGFVFRIVGDSFSAAFNSAGDALSASADAQRKLQGEDWWPEPIRVRMGIHTGLA
                     TLKQDAQGVYYEGYKTLALTQRVMSVGHGGQILLSQTSFDLVKDKSYNGIELRDMGER
                     RLKDIFNTERIYQVVAPDLISDFPPLSTLEISNHNLPSQLTSFIGRERELDVAKRLLS
                     STRILTFIGPGGTGKTRLSLQVAAEQLPEFRDGAWLIELAPLVNSTYILSTIASTFKL
                     REAQGVPLLESVTNYLRGKQLLLILDNCEHLIEACANISDEFLQNCTNIKIIASSREA
                     LGINGETVYRVPSLSLPENGSSVSSLLDSEATRLFAERAEKANAAFVLTTENASYVTQ
                     ICERLDGIPLAIELAAARVKLFTAEQIAARLDDRFKLLTGGSRTALPRQQTLRALIDW
                     SYHTLNETEANALRRLAVFSGGWTFEAAESVLGELDAMDELSGLVNKSLVNVEEQNGE
                     SRYHFLETIRQYAMEKLLESGDIVQARNRHLTYFIHYAKTADEHFVTAQRALWVARLE
                     AEHDNLRSALGWALEHDPHAALQIVCSLADFWLSRSYMSEGCSWCQSAISRAEASPER
                     GSNLDSKLAQAYSSLAMLSINRGEHQTGQIAARKAIEIERRLDNPLRLARALEFLGMS
                     LAFLGDFTMAFDSLRESESICRKFGYKDELASV"
BASE COUNT         1717 a         1666 c         1728 g         1788 t
ORIGIN      
        1 ttatagccca cagacttgcc tgggtttctt tattgtaggc tttgctattt gctttaactg
       61 gaacacccgg cgcatcccaa tagccacccg tttgttgtgc ccctacaggg tcagcagcaa
      121 gccagacgta cgtttcagcc attttttctg gcgtcatcga aaaacgccgt ttaagctcat
      181 atagcttaag catccacttt ggtaaatgtt caagccgttc agtgggaatc gccacatttc
      241 caacccgtac gcaatgggtc gttaccccgg ttcctcgcaa acgttcggcc aggtcaaaag
      301 taaacatcac ctgggcttgt ttggaatgat aataagcacg ttgcaggcta aacttccgct
      361 cgccgtaaag attatcaaat tcgataccca gaaatgggta ggtaatcaat cccttgcttg
      421 ccactgtaat tacgcggctt ggcgcactgg ctttgagtaa atccaggaac aaataggtca
      481 ttaaaaatgg cccgagatgg ttcgtagcaa aaacagtctc aagaccatcg ctgcttaaaa
      541 ttggttttcg ctgtgtgtgg tcaaagttcg ctgcattgtg aattagcacg ttaagtcttt
      601 cataacgctt ttcgaagtca gccacaaatt gacggatcga ggattgtgac gacatgtcca
      661 cctgcatcag gttgaccttg gtgctttggg ctgccttctg tatctcatcc agggcttgta
      721 accctcgctc ttttgaccgg catgccaaaa cgacagtagc acctaacttc gccaattgga
      781 cggccgcagc cttgccaatc cccgaatttc caccggttat gataatagtt ttattcaaca
      841 ggctgtattg cataaggtct ccatgtccag ttttcaaaat agcacagagg tcgtctaatc
      901 gccttaatgc tcaaggtcag gcaactcaat ccgcgataat attttgggag aggtcgaaaa
      961 acagtacgat aaatcaccga ccggttgagg gccgaatgag gagactcggc ttgataaaaa
     1021 caggcgagtc aggccgtgtg ccagctatcg ttttcaaaag catgtccacc agttgtcgcg
     1081 cgatatcgaa taccggtaga tccagagtgg tcagcggtgg ttcggaatgt tttgcgtcct
     1141 ggacaccatc gaacccacca accgcaatct cgcttccaat cctaataccc tgttcgtggg
     1201 ccgcatgcaa tgcaccgaaa gcaatttcat cagcaacgca caatatggcg tctggcgggg
     1261 tagagcgagc gaaaagtcta cgagtagcct cgtagccagc agtactcgac atatcagtag
     1321 aaattatgtt ggctcgctca atttccaatc cacttttttt cagggcagac ttacaccaat
     1381 tcaaccgctc gacgtgatcc atgagatctt taggtccgcc aagaaatgca aaccgcgaaa
     1441 agcctctgga cttgatatgt tgaacgagat tgaaataggc ttccgctcca tcaactcgga
     1501 tacacggata gtttagatct tcctgggact gaccaagtgc cacaaacgga acattctctt
     1561 ccgtgagata cttaactcgc cagtcctgtt tatggaggcg attgagtatg aagccatcca
     1621 ctttgcggct gcttacccat ctcttgtaca tgctatgctc accgtcgtct gtagttgcat
     1681 tggcgatcaa caggtcaaag ttctgatttg atagttcctc gcctaagcca gaaatgaact
     1741 cagtaaaaaa agcttcatca aagcgtttcg agatggaagg gataatgaat cccatggtct
     1801 ccgatttttg acggcgtagt tgtcgggccg cccgattggg cacatagccc attttcctgg
     1861 ccgtcttaat gacaagctca cgcgtctgct ctgctacatc gtcataccca tcgagggctc
     1921 gggacacggt ggtaatggag agatcaagtt tcttggctac atcacggatt gtaagaggca
     1981 taggttacca aaacgttttg gaagatttct ggtatattgt accattaact attttgagac
     2041 cactctatta agacttagac gcttccatag acttgatgac aaaatgagct tccctggagc
     2101 gtactgatga agctttgtaa tgagaaataa atacccagga tggtaaaaat ggattttgcg
     2161 attgaaacaa atcaactttc caagcgctac ggtgatgtgc tggccgtgga ttctgtcaat
     2221 ttacgcgtaa agcctggcga aatatatggt ttccttgggc taaatggagc aggcaagact
     2281 actaccatcc gaggattgct tgggatgatt cgtccaagtg gcggagaaat caaaatatta
     2341 gggcagacga ttgactctca tggaattgga ccgtggcgca gagttggaca tcttgttgag
     2401 tcaccatcgg cgtatcctga actgactgtg cgagaaaatc tggagatcgc cagacgcttg
     2461 catgagatat ccgatgcaga tgcggtttca cgtgtgatgg aacgacttac cctcacctcc
     2521 tatgcggatc ggcaagcggg cacactttca atgggcaatc tccagcgcct aggtctggcc
     2581 cgtgcgttat tgcatgaacc tgaactattg atccttgatg aacccgccaa tggattagat
     2641 cccgccggtg tcttggaaat tcgaacacta ttggcgtcgc ttgtaaacga aaaaggcgtt
     2701 acgatcttca tgtcgagtca catcctaaca gaggtggaca ggctggcgac gcgcctcggc
     2761 attattcatc agggccgttt gattgaagag ttggatacag ataaactcca aaagcttcgc
     2821 gccagatggc tcgaagtacg agcgcgggat cttgaagctg cacagttcgg ccttctgagc
     2881 gcagggtata cgctgacgat caaagatgac ataatcaaga tcgatgacga ttatgtgatt
     2941 gaacatcccg atgagattgc acgcatactt gtcaatgcgg gcgcaccgcc tactcatctg
     3001 gcagtggaac aacaaaatct ggaagagcat tttatgcaaa tcataggagg agcgaaatga
     3061 atggtcttgt cgcatccttg tgggcagaga ctctcaaagc gcgccgttcg aaagtgccat
     3121 tgcttgcgtc gatcggcttc acgctcgcac cgctgatgga tgggctgttc atgtttatta
     3181 tgaaagatcc agatcgagcg cgcgaaatgg ggttgcttag tgtaaaagcg caactagcca
     3241 taagcacagc agattgggct acatttttta gcgtcctgac gcaggcaatc gcagtcggag
     3301 gcgtcatcgt attttccatt gttaccgcct gggtttacgg tcgtgagttt tctgaccata
     3361 ctgcaaaaga cttaatggca ttaccaactt cccgagagaa aatcgtcaca gccaaattga
     3421 ttgtgatttc gggctgggtg gtgattacca cagtttgggt ttatctgatg ggtcttatcg
     3481 tgggaaattt cgtggacatc cctggttgga cgagtcagct cgctttgagt tcgttcctcg
     3541 acgtactggt cactgccgct atgacgttgc ttctgatgac accggtagct ttcatcgcca
     3601 gcatgggacg tggctacttg cctccgctcg gctgggcagt gcttaccata ttctttgctc
     3661 agattgtggc tgctactgga tggggcgcct ggttcccgtg gtcagttcca gctctttaca
     3721 gcggtgtggt gggacctcgc gaagagcagc tcggagcaca tagttatgtc cttgtgatcc
     3781 tgacgtcatt gattgggatg gctgcaacgt tttggtggtg gaggaacgct gatcagacac
     3841 gatagtcgat cagatgtgtt ccaatacttt cggcgcaacg ctatgggaat caacttgatt
     3901 ggagcaaaca aggaatttca gatggatggc tctgcgtgaa atctgcgcgg ccgggctagt
     3961 atcatgccgc gagagtgata tccctctgtg tagttgtaca caatatgatg ccgttccgcc
     4021 tgaggtttcg cggaatctca tcacggcata ttttcgctaa actgctccgg ctgcatcatc
     4081 aaaagacgac agaaatcggt ttccgtaatg atgccgatca gccggccatt atcgaccacg
     4141 ggcaaacctc ccactttgtg ttcaaacatc agccgcgcgg cttctgcaat cgtcgcgtcc
     4201 ggagcgatcg tgatcggatt gggagtcatg atatccttcg ctgtcagtcg gtcaatcaga
     4261 tagttaattt catatatact gagcgatgtc gcatctgaag cttttgcttc tcggaggtcg
     4321 cccagcgtca caattcccac gagcttgtcc ttttccatta ctggcagccg acggagtttg
     4381 caatcaaaca tgatccgttg ggcttctggc aaggttgttt ggggagtgat ggtcatcaac
     4441 cgcccggtca tccacttgcg aacaggatct gtgttcatat gatctctcct tttgctaagt
     4501 atgcttcttt gttggtggat cgcaaggggg cgaacaacct cattttctga ggacatatga
     4561 cctctgacgg aggagagagg gtcattatgt gcttacttaa gaaatgagag tcggaagtgg
     4621 aagaggcgaa aaatggatat taggcattga acgaacctcg cgctgaatca caatatcgcc
     4681 cagataaagg cttacaattg cgtaacctgg cacataagaa cggaacaatt actcagttat
     4741 aaaaggaaga ttcatgaccg aagcaccttc tggtactgtc tcatttctat tcacagatat
     4801 tgagggtagt actaaactgt cgcaggaata tcccgaggag atgccggctc tcctggcgcg
     4861 acatcacgaa attctcaatg agtcaattga agcacatcac ggttttgtat ttcgtattgt
     4921 cggtgattct ttttctgcgg cctttaactc tgccggtgat gcgttaagcg catctgcgga
     4981 tgcacaacgg aaactacaag gcgaagactg gtggcccgag cccatcaggg tgcgaatggg
     5041 aattcatacg ggcctggcca cattgaagca ggatgcacag ggagtatatt acgaagggta
     5101 taagaccctt gccttaactc agcgcgtgat gtctgtgggg catggggggc aaatcctgtt
     5161 atcacaaacc agctttgatt tggttaaaga caaatcctat aacggaattg aactacgcga
     5221 catgggcgag cggcggctaa aggatatatt caacacggag cgtatttatc aagtcgtggc
     5281 tcccgatctc atttcggatt ttccgccact ttccaccctt gagatatcca atcataacct
     5341 tccttcgcaa cttacctcgt ttatcggtcg agagcgcgaa ctggatgtag ccaagagatt
     5401 attatcatcc acgcgcatct taaccttcat tggtcccggt ggaaccggca aaacccggct
     5461 ttctcttcaa gtcgcggcag aacagctccc agaatttagg gatggagcat ggcttatcga
     5521 actggcacct cttgtgaatt caacgtatat actctccacg attgcgtcca ccttcaaatt
     5581 acgcgaggcg caaggagttc cactccttga gagtgttacc aattacctgc gagggaagca
     5641 gcttcttctg attctcgata actgtgagca tctcatcgaa gcctgtgcca acatctcgga
     5701 tgagttttta cagaattgca caaacatcaa gatcatcgca tccagccgcg aggccctggg
     5761 aatcaatggc gagaccgtct atcgagtgcc ctccctctcg ttgcctgaaa atggcagcag
     5821 cgtctcgtca ttgttggatt ctgaagcaac ccgacttttt gccgagcgcg cagaaaaggc
     5881 taatgcagca tttgttttga caaccgagaa cgcctcttac gtgactcaaa tctgtgaaag
     5941 gctggacgga attcctttgg cgatcgagct ggccgccgcg cgcgtcaagt tgtttactgc
     6001 tgaacaaatc gcagcccgcc tcgacgaccg ctttaaactg ctcacgggtg ggagtcgcac
     6061 agcactgccg agacaacaga ccctacgcgc tttgattgac tggagttatc acactctcaa
     6121 tgaaacggag gcgaacgctc tgcgtcgact tgcggttttt tccggcggct ggactttcga
     6181 agccgctgaa tcagtccttg gtgaacttga tgcaatggat gaattatccg gtctggtgaa
     6241 caaatcgtta gttaacgttg aagaacaaaa tggcgaatcc cgttatcact ttcttgaaac
     6301 gatccgacaa tacgcaatgg aaaaactttt ggagtcaggc gacatcgttc aggcccgtaa
     6361 tcgtcacctg acttatttca ttcattatgc caaaactgcc gatgaacatt tcgttaccgc
     6421 gcaaagggcg ttatgggtag ctcggctcga ggccgagcac gataatcttc ggtctgcatt
     6481 aggttgggca ctggaacacg accctcacgc cgcgctgcaa atcgtctgtt ccttggcaga
     6541 cttctggttg tcacgaagtt atatgagtga aggatgcagc tggtgtcagt ctgctatatc
     6601 gcgtgcagag gcttcgcctg agaggggatc aaacctggat tcaaaactag cccaggcata
     6661 ctcgtctctg gccatgctat ccatcaatcg cggcgagcat caaacagggc aaattgccgc
     6721 cagaaaggcg attgagatcg agcgtcgatt agacaatccg ttacgattgg cgcgagccct
     6781 cgagttcctg ggaatgtcat tggcattttt gggtgacttt acaatggcat ttgattccct
     6841 gcgcgaaagc gagtcgatct gccgtaaatt cggctacaag gatgaacttg cctcggttt
//
LOCUS       DASTJP010000335         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1078676, whole genome shotgun sequence.
ACCESSION   DASTJP010000335 DASTJP010000000
VERSION     DASTJP010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078676"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFQ23_10065"
     CDS             complement(<1..338)
                     /locus_tag="VFQ23_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9906979.1"
                     /translation="MADNSTFNGKYSIVTGSTQGLGEAIARLFVERGAAGLIICGRNE
                     ERGKAVVADLNSEACRVHFVQADLANVDDCRRIVAAADEHFGTVHSLVNAAGVSDRGT
                     IWDTTPELWD"
     gene            complement(328..1137)
                     /locus_tag="VFQ23_10070"
     CDS             complement(328..1137)
                     /locus_tag="VFQ23_10070"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9906980.1"
                     /translation="MYHNRPTVADMRAMKARGQKISMLYVTTLEEAAAADAAGINMLS
                     IEGRFFSAEMREAAGKCFVQVGLPYGPFGNLATAEDYLRAAFHFMQMGGDCFYCAASL
                     DIQKALCDNAVPIVAHVGLIPSQNTWTGGFKAVGKTAQSAKAVWDHVKRLEAIGCFGA
                     ELEVVPDRVAEFITKNTSMLMLGMGAGPHADAQYLFAEDVLGYNPGHKPRHSKTYRNF
                     RAEYDRLQQERISAFKELIADIQNGTYPEPKHIVPIKNDEFSAFMAVVDGR"
     gene            complement(1147..2298)
                     /locus_tag="VFQ23_10075"
     CDS             complement(1147..2298)
                     /locus_tag="VFQ23_10075"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9906981.1"
                     /translation="MEASTKYEPLADVRKELKVKWIRPKVDLAKLQELNQRKDAQGWF
                     QSLGHLGIWVFTGASVYYFWANEMWFAFLLALFFHGTVASFFVGTAPHELGHGTVFKT
                     KSLNQVFLYIYSCLGWWDQFDYAASHTYHHRYTLHPEGDRENLLPISPMVGPFFLVQL
                     FTINLLTQPGRTFGKGGLLSTIYHTILGAFGRVGDTDIPHMEWVAALHEDQPDQYKNT
                     IWWDRILLLFHGSVILASFLSGYWVFAFIISFSPFIANWGSYAVGMTQHTGLKENDTD
                     FRKATRSIRVNPLAEFLYWHMNWHTEHHMFAGIPCYNLPKLYKEIADQMPEPRTLAEA
                     WKEMRDTWAKQQDNPDYFFDTPVPPIATKARTDDALESSIGDLAPRGLQ"
     gene            complement(2418..3455)
                     /locus_tag="VFQ23_10080"
     CDS             complement(2418..3455)
                     /locus_tag="VFQ23_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9906982.1"
                     /translation="MASTATPQVAGRSLNREWLNAFIRKYAIVLIFIVMFIGMTFLTD
                     AFLQPRNLVNVVRQISVVGLIAIGVTMVIITTGIDLSSGSVLALAAVIAASLAQQPDW
                     HDAKYPGLVLPLILPILAALAVGILCGVINGWLIARFLIPPFIATLGMMTVARGFALI
                     YSNRPVSGLTDTYNFIGQGEIFKVFPIPGQPPLGIPVPVIILALVAIGAHIMLNNTRF
                     GRHIYAIGGNEQAALISGLNVGRIKIGVYTLAGLLAGLAGLVLSSRIGSGQPGLAVGI
                     ELDAIASAVIGGTSLSGGIGTIWGTIIGALIIGVLNNGLDLLNVSAYWQTIVKGSIIV
                     VAVIIDERKNR"
     gene            complement(3486..>3735)
                     /locus_tag="VFQ23_10085"
     CDS             complement(3486..>3735)
                     /locus_tag="VFQ23_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906983.1"
                     /translation="IDVGAKAEIHRLMSKLAQDGKAIIMISSELPEILGMSDRILVMH
                     EGRVGGIFERKNATQESIMQAATGHGLDAQIQQENVNA"
BASE COUNT          967 a         1032 c          864 g          872 t
ORIGIN      
        1 caatcccaca attcaggagt agtgtcccaa attgtgcctc gatcagaaac acccgcggcg
       61 ttcaccaggc tgtgaaccgt accgaaatgt tcatcagcag cagctacaat gcggcggcag
      121 tcatcgacat ttgccaggtc agcctgcaca aagtgaaccc gacaggcctc tgagttcaag
      181 tccgccacga cagcctttcc gcgttcctcg ttccgcccac aaatgatgag ccccgctgcg
      241 ccgcgttcta caaataaacg cgcaatcgct tcgcccaatc cctgcgtgct gcccgtcacg
      301 atactatatt tgccgttgaa agtagagtta tctgccatcg actaccgcca taaacgcact
      361 aaattcatca ttcttaatcg gcacgatgtg tttcggttcc ggatatgtgc cattctgtat
      421 atctgcaatc aattctttga aggcagagat acgttcctgt tgtaacctat cgtattctgc
      481 tctgaaattg cggtaggttt tggagtgacg tggtttatgc ccggggttat aacctaacac
      541 atcttccgca aacagatatt gagcatctgc atgcgggcca gcacccatgc ccagcatcag
      601 catagaggtg ttcttcgtaa taaattccgc aacgcggtcg ggcacgactt ccagctctgc
      661 cccaaaacag ccaatggctt ccagtcgttt gacgtgatcc cagacggcct tggcactctg
      721 ggcagttttg cccaccgctt taaaaccacc cgtccatgtg ttctgtgatg ggattaatcc
      781 gacatgggcg acaatcggca ccgcattgtc acaaagtgct ttctggatat caagagaggc
      841 ggcgcaatag aaacaatcac cacccatttg catgaagtga aaagccgcgc gtagataatc
      901 ttccgcagta gccaggttac cgaacggacc gtagggcaga ccgacttgta cgaaacattt
      961 gcccgccgct tcgcgcatct cggcagagaa gaagcgaccc tcaatagaga gcatgttaat
     1021 ccctgcggca tcagccgcgg cagcctcttc aagcgtggta acgtacagca tggaaatttt
     1081 ctggccgcgg gccttcatgg cgcgcatatc tgcaacagtc ggccgattat gatacatgcc
     1141 cattaactac tgcaatcctc tcggcgccaa atctccgatc gagctttcca gagcgtcgtc
     1201 agtccgggct ttggtcgcaa tgggtggaac aggcgtatca aaaaaatagt ccgggttgtc
     1261 ctgctgtttg gcccaggtat cgcgcatttc cttccaggct tcggccagcg tgcgcggttc
     1321 cggcatctga tcagcaatct ccttgtacag ctttggcagg ttatagcatg gtatcccggc
     1381 aaacatgtga tgctctgtat gccaattcat atgccagtat aaaaactcag ccaaaggatt
     1441 cacccggatc gaccgcgtgg ctttgcgaaa atcagtgtca ttctctttta agccagtgtg
     1501 ctgggtcatt ccaacagcat acgagcccca gttggcaatg aacggtgaaa atgaaatgat
     1561 gaacgcgaaa acccagtagc cactcaaaaa cgaggccagg atcacgcttc cgtgaaacaa
     1621 caaaagaata cggtcccacc agatagtgtt cttatactgg tcgggctggt cttcgtgtaa
     1681 ggccgctacc cactccatgt ggggaatgtc cgtgtccccc acacgcccga aggcacccaa
     1741 aattgtgtgg tagatcgtcg agagaagccc acctttgcca aacgttcgcc ccggttgggt
     1801 gagcaggtta atcgtaaaca actgtactaa aaagaaggga cccaccatgg gtgagatggg
     1861 caaaaggttt tcccgatctc cctctggatg cagcgtatac cgatggtgat aggtatggct
     1921 ggcagcgtaa tcgaattgat cccaccaacc caaacaactg tagatataga ggaacacctg
     1981 gttgagactc ttcgttttga aaactgtccc atgtcctaat tcgtgtgggg cggtcccaac
     2041 aaaaaagctg gcaacggtgc catgaaaaaa gagtgccaga aggaaagcaa accacatttc
     2101 gttcgcccag aaataatata ccgaggcacc ggtaaaaacc caaataccca gatgaccaag
     2161 tgattgaaac cacccctgcg catccttgcg ttgatttaat tcctgcaact tggccaggtc
     2221 aacctttggg cgtatccatt tcactttcaa ttctttccgc acatcggcca gtggttcgta
     2281 ctttgtactt gcttccatag gatatctcct ttgcgtggtc cttaaggttc caaacttcac
     2341 cgtggatttt gataaacgta gccaggtgaa ggtctgacta ttttcttcgc tcgtttccct
     2401 atcgatcgcg acacaaatta acggttcttg cgctcatcaa taattacagc cacgacgatg
     2461 atcgatcctt tgacgatggt ctgccagtag gcagaaacat tcagcaagtc aagtccattg
     2521 ttaagcacgc cgatgatcag agcgccaata atggttcccc aaattgtgcc aatccctccg
     2581 gataggcttg taccaccaat caccgcggag gcgatggcat ccaactcaat accgactgcc
     2641 agccccggct gaccagagcc aatgcgggaa gatagcacca gacctgccaa accagcaagt
     2701 aagccagcga gtgtatacac accaatcttg atacgcccca cattcaaacc cgagatcaac
     2761 gcagcttgtt catttccgcc aatcgcataa atgtgcctgc cgaagcgtgt gttgttcagc
     2821 ataatatgcg cgccaatggc caccagcgca aggataatca caggaactgg aatgccgagg
     2881 ggaggttgcc ccggaattgg aaataccttg aatatctcac cctggccaat aaagttatat
     2941 gtatcggtca acccacttac tggccgattc gagtaaatca atgcaaaacc gcgcgcgacg
     3001 gtcatcatcc ccaatgttgc aataaaagga ggaatcaaaa atctggcgat cagccagcca
     3061 ttgatcaccc cacacaggat accaaccgcc agcgccgcca gaatcggcaa aatcagaggg
     3121 agtaccagac cgggatattt cgcatcgtgc cagtctggtt gttgggccag gctggctgca
     3181 atgaccgcgg ccagggccaa cacagaaccg gacgacagat cgatcccggt cgtaatgatc
     3241 accatggtta cgccaatcgc gatcagccca acaacagaaa tttgccgcac gacatttacc
     3301 agatttcgag gttgtagaaa tgcatcggta agaaacgtca tcccaataaa catgacgata
     3361 aaaatcaata cgatggcata tttcctaata aatgcgttca accattcacg gttaaggctt
     3421 ctgcctgcga cttgtggtgt tgcagtactt gccatgattg ttctccttcc gcgtccgtcc
     3481 ggcctttacg cgttcacatt ctcttgttga atttgagcat ccagaccatg gcctgtagcc
     3541 gcctgcataa tggactcttg cgtagcattt tttcgctcaa agataccccc cactctgccc
     3601 tcgtgcatta ccaggatgcg gtcgctcatc cctaaaattt caggcagttc cgaagaaatc
     3661 ataataattg ctttgccgtc ctgtgccagt ttggacatta gcctgtgaat ctctgccttg
     3721 gcgcccacat caatg
//
LOCUS       DASTJP010000336         4976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1058236, whole genome shotgun sequence.
ACCESSION   DASTJP010000336 DASTJP010000000
VERSION     DASTJP010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058236"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFQ23_10090"
     CDS             complement(<1..607)
                     /locus_tag="VFQ23_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HET9906984.1"
                     /translation="MNPEETKPTDFIREAVAEDLKNGRYTYVRTRLPPEPNGYLHIGH
                     VKAFMIDYLIARENNGEVILRFDDTNPTKEETEFVEAIEDDARWLGIEWVKVTFASDY
                     FDELYQWAQKLVLMGKAYVDDQSPEEVSANRGTLTEPGKNSPYRDRDVEENMDLLERM
                     KNGEFPDGSRVLRAKIDMAHPNINLRDPVMYRILHEPPHHRT"
     gene            755..1096
                     /locus_tag="VFQ23_10095"
     CDS             755..1096
                     /locus_tag="VFQ23_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906985.1"
                     /translation="MNESTEKQGTFLGTYFERDSVMRLSRWAETSAWIILGIYLVYWF
                     FSFLLFIGQYTSGLFFDKGMTFLSAINFFTPYFLQPLPGVAYFFGLQAVSKGLLILLD
                     MEDNTRRGARK"
     gene            1151..1221
                     /locus_tag="VFQ23_10100"
     tRNA            1151..1221
                     /locus_tag="VFQ23_10100"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Gln,seq:ctg)
     gene            complement(1320..1784)
                     /locus_tag="VFQ23_10105"
     CDS             complement(1320..1784)
                     /locus_tag="VFQ23_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906986.1"
                     /translation="MNTVSILLVEDHEVFAKALIRALGANQELDVVATAKSAEEALEQ
                     LDTVKVDLVLADVSLPHMNGIDLVKELRQKFPHLRCAVLSGHLALDYVQRALDAGANG
                     YMVKDNPAGILEGIQHILDGEVYVSKEVRDSFNGPHSALNYRDNPRGTASNA"
     gene            complement(1826..2221)
                     /locus_tag="VFQ23_10110"
     CDS             complement(1826..2221)
                     /locus_tag="VFQ23_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9906987.1"
                     /translation="MRSILLVEDHSTFASVLVRLLLTTKDMQITLVATSAESALQELR
                     SQRFDLVLVDVSLPEINGIALVGMIRDQFPGLPCLMLSGHALAHYKNRALDAGARGYI
                     LKDNAKGIIEGIRRVLNGEIYVSKELREG"
     gene            complement(2223..>4976)
                     /locus_tag="VFQ23_10115"
     CDS             complement(2223..>4976)
                     /locus_tag="VFQ23_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9906988.1"
                     /translation="EEGIRIWSIGCATGEEAYGLAMLLMEESEKQDFHTPLQVFASDL
                     DERSISRAREGLYPAAIEADLSPERLERFFTREGEYYHVKRELRDIVLFTNHNIMRDP
                     PFSRQDLIACRNVLIYLQRSVQDKLFDIFHYSLKPGGYLFMGSSESAEHLPALFSIVD
                     KTHRLYQARPWNGEAPHVPSMPLSSRKALQPQEEYVIHRPHEARFPEGSALLEEQHRT
                     ALENYGPPSVIVNERYGILHISETAGRYLHHPKGTITGDLLALVRPELQPELRSVLFR
                     AFEKARATVSRPVAVQLNGHRRRVIITVRLSEQSLSQMAERQALVFFIEDEEESIADS
                     VADIGVPRNEADRDETVAQLQSEIRNLREQLQITTEEYDSSNEEMKSANEELQSINEE
                     YRSAAEELETSKEELQSVNEELQTVNSEMRSKLDEISQAHQELENLMGATEIATLYLD
                     RELNIQRFTAGVQELFSIRSLDRGRRISDLAHQLGYENFSKDAEQVLRNLIPLEREIE
                     KGGGNWFVMRMRPYRTVEDRIEGVVVSFIDITKLKQTEQEMVRIKETLEHRVEERTRE
                     LDEASRQITQARDLFENLFALNPVPTSITRLEDGVFINVNEAYLKYFSLTGEDLIGHA
                     AQELGLPLEPTIRPKLIARVQQQGFVQNIEMETKLPSGEIRTILVSVQVVKVSNTDAL
                     MLAFIDITEHVLTAKQARTAANSLSASDRMERQRISQVLHDDLQQNLFAVKMQLSFLD
                     DAIAKGKVEAANVDLKQLDQWLAEAIATTRQLSIDLSPPVLPAGEGLTESLIWLASQM
                     KTQYNMEVTLDPNGTQAVLDDDVRSLLLQSVRELLFNVVKHSGTLNAQVALEQMNGQV
                     KIIVSDQGKGFDLQSKTDKDFNGLKKMRNRLYLVGANLEIQSAPDTGTQITIEAPVAD
                     TME"
BASE COUNT         1101 a         1331 c         1251 g         1293 t
ORIGIN      
        1 cggtgcgatg gtggggaggc tcgtgaagaa tgcgatacat gaccgggtct cgcaggttga
       61 tattgggatg cgccatgtcg atttttgcgc gcaggacgcg tgagccgtct gggaattcgc
      121 cgtttttcat gcgttccaac agatccatat tctcttccac gtcgcggtcg cggtaaggag
      181 agtttttacc cggctctgtg agcgtcccac gattggcgct gacttcctca ggcgactggt
      241 catccacgta tgctttaccc atcaaaacca gcttctgggc ccactggtat aactcatcga
      301 agtaatcgga ggcaaacgtg accttcaccc attcgatgcc cagccagcga gcgtcatctt
      361 caatggcttc cacaaattcg gtctcttctt tcgtcggatt cgtgtcgtcg aagcgcagaa
      421 tcacctcgcc gttgttttcc ctggcaatga ggtaatcgat catgaaagct ttgacatgcc
      481 caatatgcaa atagccgttc ggttcgggcg gcaggcgcgt gcggacgtag gtatagcgcc
      541 cattttttag gtcttccgcg acggcttcgc ggataaagtc ggttggcttg gtttcctctg
      601 gattcatcta ctcgctacct taaattattg aaaatcacta cttttggacc gtcctcctgt
      661 gctgcgatct gggaacaatt gccccgcaga tacggtcgtt aggataatta taacaaaagc
      721 cagttgttat ataatcacat ctggaggtac cctcatgaat gaatcaactg aaaaacaggg
      781 gacatttttg ggcacatatt ttgaacggga ttcggtcatg cggctgtcgc gctgggccga
      841 gacatctgca tggataattt tgggcattta tcttgtatat tggttctttt cgttccttct
      901 ttttattggt caatatacga gcggcttatt tttcgataaa gggatgacgt tcctgagcgc
      961 catcaatttc tttacaccct acttcttgca acctctgccg ggagtcgcgt acttctttgg
     1021 gttgcaggcg gtttcaaagg ggctactgat tctgctggat atggaagata acacgcgacg
     1081 tggtgcacgc aagtaagtgc cttgcccctg ccagcatcgc atgatacaat accgcccgct
     1141 ggcgaagatt tgaggagtcg tccaacggca ggacacagca ctctggatgc tgttatcttg
     1201 gttcgaatcc aggctcctca gctgaaaaga atcctccaca atggaggatt cttttcttta
     1261 aacggaaaac gcccacatga ttggagcgtt tcttacaata agcttgtgaa tacgattcgc
     1321 tatgcgtttg aggcagttcc gcgcgggtta tctcggtagt tcagtgcact gtgcggcccg
     1381 ttgaacgagt ctctcacttc cttgctcaca taaacttcgc catccaggat gtgctgaatg
     1441 ccctcgagaa tgcctgccgg gttatccttg accatataac cattggcgcc ggcatccaga
     1501 gcacgttgga cgtagtccaa agccagatga cccgaaagca ccgcgcaacg cagatgtggg
     1561 aatttctggc gcaattcctt taccagatcg atgccgttca tgtgtggcag ggagacatcg
     1621 gcgagcacca ggtcgacttt taccgtgtcc aattgttcga gagcttcctc ggcgcttttc
     1681 gcggtggcaa ccacgtccaa ttcctgattt gcacctaaag ctcggatgag tgcttttgca
     1741 aagacttcgt gatcttccac taaaaggatc gaaactgtat tcaagctaca cctttcacgc
     1801 tatccacggg cgcagtatat cacatttacc cctcacgtaa ttcctttgat acgtagattt
     1861 ctccattcaa cacacgacgg atcccttcga taattccttt ggcgttgtct ttgagaatat
     1921 aaccccgcgc accggcatcc agggcccgat tcttgtagtg cgccagcgca tgcccggaga
     1981 gcatcaggca gggcagcccc ggaaactgat cacgtatcat tcctaccagc gcgatgccgt
     2041 tgatttcagg cagggaaacg tccaccagaa ccaggtcaaa tctctgtgaa cgcagttcct
     2101 gcaacgcgct ttcggcagag gtcgctacca gggtaatctg catatctttc gtggtcagta
     2161 acagacgaac gagaacactg gcgaatgtgc tatggtcttc cacaagtaaa atggaccgca
     2221 ttctattcca tcgtgtccgc caccggggct tcgatggtaa tttgggtgcc agtatctggg
     2281 gcagattgta tctcaagatt ggcgcccacc agataaagac ggtttcgcat ttttttcagc
     2341 ccgttgaaat ctttgtccgt ctttgactgc aaatcaaaac ccttgccctg atcgctcacg
     2401 ataattttta cctgaccgtt catttgttca agagctacct gagcattcaa cgttccggaa
     2461 tgcttcacaa cattgaacag tagttctcgc accgattgga ggagcaggct gcgtacatca
     2521 tcatccaaaa cagcctgcgt gccatttgga tcaagggtca cctccatatt gtattgggtt
     2581 ttcatctgtg aggccagcca gatcagcgat tcggtcagac cttcccccgc cggcaacaca
     2641 ggcgggctca aatcgatact cagctggcgg gtggtggcaa tggcttctgc cagccattga
     2701 tccaattgtt tgagatcgac attggctgcc tcaactttac ccttcgcaat cgcatcatcc
     2761 aaaaaggata actgcatctt gaccgcgaaa aggttctgtt ggagatcgtc atgcaggact
     2821 tgtgaaatcc gctggcgctc catccggtct gaggcgctca gactgttggc agccgtgcgt
     2881 gcctgttttg ctgtgagcac atgctcggtg atgtcgataa acgccagcat cagtgcatcc
     2941 gtattactga ctttcacaac ttgaaccgag accaggattg ttctgatttc gccagatgga
     3001 agtttggtct ccatctcgat gttctggaca aacccttgtt gttgaactcg agcgatcaat
     3061 tttggacgga tggttggctc aagtggaaga cccagctctt gcgctgcatg tccgatcaga
     3121 tcctccccgg tgaggctgaa atatttcaga taggcttcat tgacgttgat gaatacaccg
     3181 tcttccagcc tggtgatcga agtgggcacc gggttgagcg cgaagagatt ctcgaacaga
     3241 tcgcgtgcct gcgtgatttg gcggctggct tcatcgagtt cacgggtgcg ctcctccaca
     3301 cggtgctcaa gcgtttcctt gatccgaacc atttcctgtt cagtctgctt aagtttagtg
     3361 atgtcaataa atgaaactac cacgccctcg atgcgatcct ccacggtgcg atagggacgc
     3421 attcgcatca caaaccagtt gccaccaccc ttttctattt cgcgctccag tgggatcagg
     3481 ttgcgtaaga cctgctctgc atccttcgaa aaattctcat atcccagctg gtgggctaag
     3541 tcactaatgc ggcggccgcg gtccagggac cggatgctaa acaattcctg aacccctgct
     3601 gtaaaacgct ggatgttcag ctcacgatcc aggtagaggg tggcgatctc ggtcgcgccc
     3661 atcagatttt ccaactcctg atgagcctgt gagatctcat ccagcttgct cctcatttcg
     3721 ctgttgaccg tttgaagttc ttcatttacg gactgcaatt cctccttgct ggtctccagc
     3781 tcctcagccg cagagcgata ctcctcattg atggactgca gttcctcgtt ggccgacttc
     3841 atctcctcgt tggagctatc gtattcctcg gtcgtgatct gcaattgctc tcgaagattt
     3901 cggatctcag actgtaactg cgccaccgtt tcatctcggt ctgcctcatt gcgaggcacg
     3961 cctatgtctg ccacagagtc tgcgatactt tcttcctcgt cttcgataaa aaagaccaaa
     4021 gcctgtcgct ccgccatctg gctgagactt tgctcactca ggcgcacggt gatgatgacg
     4081 cgacgccggt gaccattgag ttgaactgcc accggccggc tgacggtagc tctggccttc
     4141 tcgaaggcac ggaagagcac cgaacgcaat tccggttgca attcaggacg caccagcgcg
     4201 agcaaatcgc ctgtgatcgt ccccttggga tggtgcagat atcgcccggc agtttctgat
     4261 atgtgcagaa tgccatacct ttcattgaca atcacgctgg gaggaccata attttccaat
     4321 gctgttcggt gttgctcctc caaaagcgct gagccttccg ggaacctcgc ctcatgcggc
     4381 cggtggatca catactcttc ctgcggttgt aaagctttcc tcgaagacag aggcatggaa
     4441 ggaacatgtg gtgcctcacc attccagggc ctggcttgat aaagccggtg cgttttatca
     4501 acgatactaa ataaagcagg caggtgctcg gcagattccg aactgcccat gaagaggtag
     4561 ccgcccggtt tgagcgaata gtgaaagata tcgaacaact tgtcctgcac ggagcgctgt
     4621 aaatagatca acacattccg acaggcgatc aggtcctgcc gtgagaaagg cgggtcgcgc
     4681 atgatgttgt gattggtaaa gagcacaata tcgcgcagct cacgtttcac atgataatac
     4741 tctccctcgc gggtaaagaa tcgttcgagg cgttcagggc tgaggtcagc ttcgatggca
     4801 gcgggataga gtccctcgcg ggcacggctg atcgacctct catccaggtc tgaagcgaag
     4861 acctgaagag gggtatggaa atcctgcttt tcggattctt ccatgagcag catggcaagc
     4921 ccgtaggctt cctcgcccgt cgcacatccg atagaccaga tacgaatgcc ctcttc
//
LOCUS       DASTJP010000337         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_117841, whole genome shotgun sequence.
ACCESSION   DASTJP010000337 DASTJP010000000
VERSION     DASTJP010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117841"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="VFQ23_10120"
     CDS             complement(<1..1484)
                     /locus_tag="VFQ23_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9906989.1"
                     /translation="MPASRWHTGLNELFQWMGEDDNDLLSMIIPSMFSRFQKQDADSA
                     GPNAPQVSGTNKYPDVLPILPLRGVVVYPQTAVPLTVGQPRSIKLVDEVVGGDKLVGL
                     VAAVNPELESPGPADLYQIGTIATVHRLLRAPDGTIRLLVQGLDRFRVGEFIAEEPYL
                     KARIELAPETMEEGLEIDALARNARDQFQQITQMIPSFPEELASSITSVEDALQTAYT
                     IANFQRMDIKDSQEILELDSVAEKLKKLIGLLVREAEVLQLGQKIQNEARGEIEKVQR
                     EYFLREQMKAIQKELGEKDEQVAEAEEFRKKIEEAKMPEEAEKQARRELERLSRLPTA
                     AAEYGVIRTYLDWLVSLPWSKLTEDNLEIPHARDILDKDHYGLKDVKERILEFLAIRK
                     LRLDRRDETRAPSEDKIRREREGVILCFVGPPGVGKTSLGQSIARAMGRKFVRASLGG
                     VRDEAEIRGHRRTYIGSMPGRILQSLRRIESRNPVFMLDEIDKLT"
     gene            complement(1490..1888)
                     /locus_tag="VFQ23_10125"
     CDS             complement(1490..1888)
                     /locus_tag="VFQ23_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9906990.1"
                     /translation="MPTVVRKSFSAVTESRREIIHSISWQVSANVWCPPTDVYETSDG
                     FVVKVEVAGMREDDFEVGIEDNMLMISGVRPTLNERCAYHQLEIRSGKFEVAVEIPAP
                     IKVEWSVAEYKDGFLMIHLPKANHKQTGVA"
     gene            complement(2487..2924)
                     /locus_tag="VFQ23_10130"
     CDS             complement(2487..2924)
                     /locus_tag="VFQ23_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906991.1"
                     /translation="MNSDHVLAQIRRLLLVISAGVFVMTVIELFFLSHWSETIQWLPF
                     ALSAVGLVTLVLAYFRPDPRTISVLRWSMVVIGLCSLIGIYEHMANNLGFQMEIQPNA
                     TTWEYIRATLEGANPVLAPGILTLGAAIGWTATYQHNLHQDER"
BASE COUNT          760 a          819 c          662 g          776 t
ORIGIN      
        1 gtcagcttgt cgatttcatc gagcatgaac accggattcc tcgactcgat ccgtcgcaat
       61 gactgcagga ttcgaccagg catcgagcca atataggtcc gtcggtgccc ccgaatctct
      121 gcctcatcgc gcactccgcc cagggacgcc cgtacaaatt tccgtcccat tgcgcgcgcg
      181 atggattgac cgagcgatgt tttgccaacc cccggtggac ctacaaaaca tagaatcacg
      241 ccttcacgct cgcgccgtat tttatcttca gatggcgctc gggtctcatc tctgcggtcg
      301 aggcgcagtt ttcgaatggc aagaaactcc agaatgcgtt ccttgacatc tttcaaccca
      361 taatggtcct tatccaaaat atcacgagca tgaggaattt cgagattatc ttcggtcaat
      421 ttggaccagg gcaatgaaac cagccaatcc aaataggtgc ggattacgcc atactccgcc
      481 gcagcggttg gtaaccgtga caggcgctcc agttctcgtc tcgcctgttt ctcagcttcc
      541 tcgggcattt ttgcttcttc gattttcttg cgaaattcct cggcttcagc gacttgttcg
      601 tccttctcac ccagttcctt ttggatggcc ttcatctgct cgcgaaggaa gtattcgcgt
      661 tgaacctttt cgatctcacc gcgggcttca ttttgtatct tttgcccgag ttgtaaaact
      721 tccgcctctc ttaccaggag accaatcagc tttttgagtt tctcagcgac cgaatcaagc
      781 tcaaggattt cctgggaatc cttgatatcc atccgctgaa agtttgcaat cgtataggcg
      841 gtttgcaaag catcttcaac cgaagttatc gagctggcca gctcctccgg gaaggaggga
      901 atcatttgtg taatttgctg gaattgatct cgtgcgtttc gagccagcgc gtcgatttca
      961 agtccttctt ccatggtctc aggcgcgagt tcaatccttg ccttgaggta aggctcctcc
     1021 gcaataaatt cacccactct gaaacgatcc aggccttgta ccagcaaccg gatggttcca
     1081 tcgggtgcac gcaacagccg atggacagta gcaattgtcc cgatctgata aagatctgcc
     1141 ggacctggcg attcaagctc cggatttacg gccgccacca gccccaccag cttatctccg
     1201 ccgacaactt catccaccag cttgatcgag cgaggctggc caacggtcaa cggcacagcc
     1261 gtctgtggat aaaccaccac tcctctgagc ggaagaattg gaagaacatc cgggtattta
     1321 tttgtacctg aaacctgagg cgcattcggg cccgccgaat ctgcatcttg cttctgaaaa
     1381 cgcgaaaaca tggatggaat tatcatagac aacagatcat tgtcgtcctc gcccatccat
     1441 tgaaaaagtt cgttcaagcc ggtgtgccag cgtgaagcag gcataggtat taggcaaccc
     1501 ccgtctgttt gtgatttgct ttcggcaaat gtatcattag aaatccatcc ttgtattcag
     1561 ctacagacca ttccactttt atgggtgcag gtatctcaac cgcaacctcg aacttgcctg
     1621 accttatttc cagttggtgg tacgcacatc gctcgttaag agtgggtcgg actccgctga
     1681 tcatcagcat attatcttca attccaacct caaaatcgtc ctcacgcatc cccgccactt
     1741 ccacttttac gacaaatccg tctgaggttt cataaacatc cgtcggcgga caccaaacat
     1801 ttgcgctgac ctgccagcta atcgaatgga tgatttcgcg gcgtgattcg gtcactgcgg
     1861 aaaacgattt acgaacgacc gtaggcataa actttctcct tctgggaaac tggtcttaca
     1921 gaaaatcgca ggagtatagg cagaaatgga actccaaacg aacagtgtgg aggaacggac
     1981 aaccacttcc agcttgtaat tatacttcca tctagtctat tcagtgcgaa aaataaggga
     2041 gttgctaaga gagtgagtga aaatctattt agccacagag agcactgagc cctttgaccc
     2101 cgcatgattt gctaagcaaa tcacgttcgg ggcaggtgga aaaaaactca aaaactcggt
     2161 ggctaacaca acaaattcgc cactcccaaa aataagaccg ttgatcggat ttattatgag
     2221 ggatggcctt taccgtctgt aattgccatt ccatatcgct actggtgatt agccacaatt
     2281 cccttcatac aaccttgatg aattgcgcca tctttttcca cctgaagtgt tgtgagcttg
     2341 ctcagccgca agctcaactt ttacgtcctg aaatcttatc tggcttctat cgcttccgga
     2401 tcgggtaagg ggattccgag caaagtgact gtaaaaatat tcaatcagcc gttgtacgtt
     2461 ctcagtttct atcggcgtaa cttcgatcac cgctcatctt gatgtaaatt atgttggtag
     2521 gttgcggtcc agccaatagc tgcacccaac gtaagaattc ctggcgccag caccggattt
     2581 gccccttcca acgttgcccg aatgtactcc cacgtcgtgg cattcggttg gatttccatc
     2641 tgaaaaccaa gattatttgc catatgctca tagataccaa tcaggctaca aagcccaatc
     2701 accaccatcg accatcgtaa aacgctgatc gtcctgggat ccggtctgaa atacgccaga
     2761 accaaggtga ccaaacccac cgcgcttaat gcgaaaggaa gccactggat ggtttcactc
     2821 cagtgactga gaaaaaataa ttcaataaca gtcattacaa acacaccggc agagatgacc
     2881 aaaagcagcc tgcgtatttg agcaaggacg tggtccgaat tcattaattg tctcccaaat
     2941 agcttttagc agtttctaat tcgtccgcag agacatattc actcagcttc gtcagaaaag
     3001 catcggccga tgcaaac
//
LOCUS       DASTJP010000338         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1274218, whole genome shotgun sequence.
ACCESSION   DASTJP010000338 DASTJP010000000
VERSION     DASTJP010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274218"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFQ23_10135"
     CDS             <1..788
                     /locus_tag="VFQ23_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9906992.1"
                     /translation="EMMAMRGNRISMIFQQPQTSLNPVFRVGEQISEVLNIHQDFGRE
                     AGKKRAVELLKLVGIPDAERRAEAFPHELSGGMAQRVMIAMALACVPDLLIADEPTTA
                     LDVTIQAQILDLMRNLRSQLGSAVILITHDLGVIAEMADRVAVMYAGEVVEQTDVISL
                     FKEPLHPYTVGLIGSTPVLGNIREKLEVIPGSVPNLVRLPPGCRFQPRCQACVKYNMS
                     ICAEKQPDLIQLEDGHKIRCWLYQDSPEDGHAAPLKNQISLKK"
     gene            796..1794
                     /locus_tag="VFQ23_10140"
     CDS             796..1794
                     /locus_tag="VFQ23_10140"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET9906993.1"
                     /translation="MSINSSKDNYELVVVKDLVKYFPVRAGVLQRVKAYVQAVDKVSF
                     TVKEGETLGLVGESGCGKTTVGRSILRLIEPTSGSVFINGEDVMQMRSQSLKAARRNM
                     QIIFQDPYASLNPRLPIGESVMEGLHIHNVGHPKERWEIALRMLKKVGLEDYHARRYP
                     HEFSGGQRQRIGIARALALQPKFIVCDEPVSALDVSIQSQVLNILKDLQNEFGLTYLF
                     IAHNMSVVEHISDRVAVMYLGKMVELADREELFRKPLHPYTQALLSAIPIPDPTLKRE
                     RIILKGDVPSPLNPPKGCRFHPRCPWAIDICSQEEPEFKELLPGHFVACWVAEKNL"
     gene            1872..>3679
                     /locus_tag="VFQ23_10145"
     CDS             1872..>3679
                     /locus_tag="VFQ23_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9906994.1"
                     /translation="MKKYSVVFLIIVLLSACIPQATDLPNIPSTPTASQPTPIFTPTP
                     EQRSLTICLGEEPNTLYPYGSLNSAARSVLSAIYDGPMDVVEYGYEPIILEKIPTLED
                     GDAQVSPVIVRAGDLVVSSNGDLVTLAAGVKIRPSSCRSEDCAITYDGSSAIEMDQLV
                     VTFTLLEGLMWSDGEPLTADDSIYSFELASNDDTPVSKFVIDRTQTYEAADDLTIQWW
                     GRPGFIDAEYFTNFWMPLPRHAWGEFPPVDLTRLEISSRFPLGWGPYIMDEWERGQQI
                     HLIKNLNYFRAESGFPRFDELTFLIFPDTNSALTSLVDGTCDVLDPSVRLDGQVGLLQ
                     QMETEEQAELLTSQTMTMEWLAFGILPASYDNGFNAREDRPDFFGDERTRQAIALCLD
                     RQKVVDTVLFGLSQVPDSYLPADHPLHNGNLQAYEFNPARGNQILEQAGWIDNDNNPS
                     TPRQALGVTNVPAGTPLVLNYITSSATQRHQVVELLTLSLAECGIGLNSTYYSAVDFY
                     EQGPTGPLFGRNFDLAEYAIGVSSLEPQCSWFTTSQIPREENNWVGTNVSGYQNPQFD
                     SACEKALQSLSSDPEYALHQEAQAIFATDLPSIPLY"
BASE COUNT          902 a          914 c          937 g          926 t
ORIGIN      
        1 acgaaatgat ggcgatgcgg ggcaaccgca tttcaatgat cttccagcag ccacagactt
       61 ccttgaaccc tgtattccgc gtgggcgaac agatcagtga agttttgaat attcaccagg
      121 atttcggacg ggaagccggc aaaaagcgcg cggtcgaatt actcaaactg gtgggaatcc
      181 cagatgcgga acgacgtgcg gaggcctttc cgcatgagct ttcaggtggt atggcacaac
      241 gtgtcatgat tgctatggcg ctggcatgtg ttcccgatct tctcatcgca gacgaaccca
      301 caacagcgct ggatgtgacc attcaggcgc aaatcctgga cctgatgcgc aacttgcgct
      361 cgcagcttgg ctccgctgtc atcctcatca cccacgactt gggcgtgatc gctgagatgg
      421 cagatcgagt tgcagtgatg tatgcgggcg aagtcgtgga acagacggat gtcattagtc
      481 tctttaaaga accattacat ccctacacag taggcctgat tggctcaact ccggtcctgg
      541 gcaatattcg cgaaaagctc gaagtgatcc ctgggtcggt tccaaacctt gtgcgtttgc
      601 ctcccggatg ccgattccag ccgcgttgtc aggcatgtgt gaaatacaac atgtcgattt
      661 gtgcagaaaa gcaacccgac ttgatccagc ttgaagatgg tcataagatt cgctgctggc
      721 tttatcagga ttctcctgag gatggccacg ccgcgcccct taagaaccaa atctcattga
      781 agaaatgatt ctgacatgtc aattaattca tccaaagata attatgaact tgttgtagta
      841 aaggatctgg tcaagtattt tcctgtgcgc gctggcgtgt tgcaacgagt gaaagcctac
      901 gtacaggcgg ttgacaaagt cagtttcaca gtaaaagagg gtgagacact cgggctcgtt
      961 ggcgagtcag gctgtgggaa aaccaccgtc gggcgttcca ttcttcgtct gattgagcca
     1021 acctcgggct ccgtctttat taatggggaa gatgtgatgc aaatgcgatc acaatcgctt
     1081 aaagccgcgc gccgcaatat gcaaattatt tttcaggatc cctatgcatc attaaaccct
     1141 cgcttgccga ttggtgaatc ggtgatggag ggtctgcaca ttcacaatgt aggtcatcca
     1201 aaagagcgct gggaaattgc actccgcatg ttgaaaaagg tagggcttga agattaccac
     1261 gcacgtcgtt atccccatga attctcaggg gggcagcgcc agagaatcgg tatagcccgg
     1321 gcgctggcat tacagcccaa gttcattgtt tgcgatgagc ctgtctctgc tttggatgtt
     1381 tccatccaat cacaggtctt gaatattctg aaagacctgc aaaacgaatt tggtcttacc
     1441 tatctgttta tcgctcataa catgagtgtc gttgaacaca tttcagatcg cgtggctgtg
     1501 atgtaccttg gcaaaatggt ggagctggct gaccgcgagg agctcttccg caaaccatta
     1561 cacccctata ctcaggcgtt gttatctgcc ataccgatcc cggacccaac gctaaaacgc
     1621 gagcgcatca tcttaaaggg cgatgtaccc agcccgttga atccgcctaa gggctgccgc
     1681 ttccatccaa gatgtccgtg ggcgatcgat atatgctcac aagaggagcc tgagttcaag
     1741 gaattgctcc ccggtcattt tgtcgcttgc tgggtagctg aaaaaaatct gtaagccgaa
     1801 ctagacctgt ttccgatgtg ggaacaggtt tagttatata atatggggaa tttgtagaag
     1861 cggaatttca tatgaaaaaa tatagtgtag tgttcttaat tatcgtactg ctctccgcct
     1921 gtataccaca ggcgactgat ctgccgaaca tcccttccac accgaccgct tcacaaccca
     1981 caccgatctt cacgccaacg cctgaacaac gatcattaac gatttgcctc ggtgaggagc
     2041 caaacaccct gtatccgtat ggcagcctga attcagctgc acgcagtgtg ctctcggcga
     2101 tctacgatgg ccctatggat gtagtggaat atggatatga gcccatcatt cttgagaaga
     2161 ttccgacgct tgaagacggc gacgcgcagg taagtcctgt aatagtgaga gcaggggatc
     2221 tggtggtttc ctcgaatggc gatctggtaa cactcgcggc aggcgtgaaa attcgaccca
     2281 gcagctgccg cagcgaggat tgtgccatta cctatgatgg ctcaagcgcc atcgagatgg
     2341 accagcttgt ggttacattc accctgctag agggactgat gtggtcagac ggcgaaccac
     2401 tcacggcaga tgattcgatc tattcctttg aacttgcatc gaatgacgat acgccggttt
     2461 caaaattcgt gatcgaccgt actcaaactt atgaagctgc cgacgatctg acgatccaat
     2521 ggtggggacg gcccgggttt attgatgccg aatatttcac caatttttgg atgcctctcc
     2581 ccagacatgc ctggggagaa ttcccgcccg tagacctcac acggcttgaa atttcctcac
     2641 gctttccttt gggctgggga ccttacatca tggatgaatg ggaaagggga cagcaaattc
     2701 atcttattaa gaatctgaat tatttccgtg cagagagtgg ttttccaaga tttgacgagt
     2761 tgacgtttct gattttcccc gatacaaact cagcgcttac ctcacttgtc gatgggacct
     2821 gtgatgtcct ggatccatct gtacgattgg acgggcaggt ggggctgctc caacagatgg
     2881 aaacagaaga gcaggcagaa ttgttaacat cgcagacgat gacgatggaa tggctggctt
     2941 ttgggatcct tccggcctct tatgataatg gtttcaacgc cagagaggat cgcccggatt
     3001 tctttggcga tgaacgcacc cgccaggcaa ttgccttgtg tctggatcgt cagaaggttg
     3061 tggatactgt cctttttgga ctttctcagg tgccagatag ttaccttcca gcagatcatc
     3121 cacttcataa cggcaacctt caggcttatg agttcaatcc ggcacgaggc aatcagatcc
     3181 ttgaacaggc cggctggatc gataatgata ataatccatc cacgccacgc caagccctgg
     3241 gtgtgaccaa tgttccggca ggaacgcctc tggtccttaa ttacattaca tcctctgcaa
     3301 cacaacgcca tcaggtggtg gagctactaa ctttgtccct tgcggaatgc ggcattggat
     3361 tgaattcaac atattattct gcagtggatt tctatgaaca gggaccgact ggaccgctct
     3421 ttggccgcaa ctttgatctc gccgaatatg caattggggt gagcagtctg gaacctcaat
     3481 gcagttggtt tactacatca caaattccac gggaggaaaa caattgggtt gggacgaacg
     3541 tcagtggata tcaaaatccg cagtttgaca gcgcgtgtga aaaggctctt cagtcattgt
     3601 cttctgaccc cgagtatgcg ctgcatcagg aggcacaggc gatcttcgcg accgacctgc
     3661 cgtctattcc tctatacca
//
LOCUS       DASTJP010000339         8363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_959940, whole genome shotgun sequence.
ACCESSION   DASTJP010000339 DASTJP010000000
VERSION     DASTJP010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959940"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFQ23_10150"
     CDS             <1..469
                     /locus_tag="VFQ23_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906995.1"
                     /translation="AWSKYAGILFSALIIISAILFNYELYPSECCILVGADDVVAIDW
                     MDKNLPPDASVAISAVELIVLASDSFQGYVGGDAGIWITPLTSQPTIPFLNHSDFSQA
                     TTLHTLCELNAHYLYIGETGQVFDDAQITAQPQWYKALLSMPKSKVYQIIGCT"
     gene            complement(572..2641)
                     /locus_tag="VFQ23_10155"
     CDS             complement(572..2641)
                     /locus_tag="VFQ23_10155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9906996.1"
                     /translation="MQADKFSRYEIREELGQGGMATVYRAYDPLFEREVALKILKQEL
                     LANSQIRERFERETKIIAKLEHAAIVPVYDVGRDKDQLFFVMRYMSGGSLSDRIQNKS
                     MTFSEIAYIIQRVAAALDYAHSKDVIHRDLKPGNILFDEYNNPFISDFGIAKISRASI
                     KLTHSGIIGTPTYMSPEQAQGEEVDGRSDIYSLGVILFEMLSGKTPYEATTPLGMAFK
                     HAAEPVPHILRVNPDLPTGIETVIEKVMAKDRDQRYSSAVEFANDFSATIAGNAHLVA
                     PIPKPVQINDAVLTPPPSTGAHKRQPISRFWMFGGFIILTLAALWGYPRFAASFNPTS
                     ETATSTFTASPPTSTPTEFIVPTSTATAVNEVTPTVNPILTIGGADKIALSANNDIFM
                     MDMDGRNILQLTNTNVPKFDLQWLPDGNELLYGEGNCFYTINVEVPEVASEKLACFNE
                     EDFDGFRVSPDGTHVAISIGNRLIVLRFDKQTLSTVATTFELQGLEDLCLDYSDVSVK
                     GALWSATGQELAITYESVVGQRLGDTIRVLDIDTRRCQAADPLIMDEFPAKHFMPEGY
                     ERYPLLPSYHWDGNQRFLFNSFKRNTAYGELYLYDMSTMTERKVNPIDGVCCYNSAIF
                     SPDGTHILFVFQDVRRGENSITQLYYVPIDQMDSGITFTPVRLPLQFFLDLREDIELA
                     LRPVAPQ"
     gene            2893..3222
                     /locus_tag="VFQ23_10160"
     CDS             2893..3222
                     /locus_tag="VFQ23_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9906997.1"
                     /translation="MKRKLSMLFVVLLLALVMVIPASAGRGNETPGVVYVTSQGLYYD
                     TFVVKDPLPANGRFQQIVNGTTEFGPGDPGYLGGRWWDDTNMNGIMDATDHYFLCPLL
                     GPGRETP"
     gene            complement(3399..4568)
                     /locus_tag="VFQ23_10165"
     CDS             complement(3399..4568)
                     /locus_tag="VFQ23_10165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9906998.1"
                     /translation="MSILFQFEVETIALFVAMTFFVQATVIGAQAYLIRELKQYRGVI
                     AALLANLCAAVGLMIRLFAEQLPIFLTIIVSNMLILTGTAMFYIALSQFTGFAYKKIA
                     LTGIFVSVLAFLLYFTYGVDEVGKRILVISLGSIAMVSLLIHQLWQIQKTALKLSANL
                     MLVSFLVYGLFLVIRTIAILLQPPLEVSGLSPVQSATYLFAFALSFFWSMGFILMVSQ
                     RLRNDLMEVATIDVLTRIPNRRATQTFLERELSRSQRDHSEFSILLIDLDNFKQVNDR
                     WGHAMGDYVLVKTAGIFQSMIRKHDWAGRWGGEEFLIILPSTPDAQALAERLRREIAN
                     SEYKQGDITFNITISIGVARGTSANQIDELLTNADQALYTAKLTKNTVSMANVEK"
     gene            complement(4665..5306)
                     /locus_tag="VFQ23_10170"
     CDS             complement(4665..5306)
                     /locus_tag="VFQ23_10170"
                     /inference="COORDINATES: protein motif:HMM:NF044435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9906999.1"
                     /translation="MPKQTFLNLPEEKRRAFLEIALDEFANNDYNTASVSKIVEKAGI
                     AKGSVYQYFEDKQDLFMHLLDVSNQEMMAVVEQSPSPDPNADFFEALRWQMSAAVRAS
                     ARFPIHSKLARRAYTSPLPFRDSILEKAKKVREEHFQGLVARAQASGNLDPHLDPAVV
                     SFMVQGLMSEMGSFLQSKFGKRNGDWVARPEVSDVFDQVIDVLKNGLTSRSAE"
     gene            complement(5275..6132)
                     /locus_tag="VFQ23_10175"
     CDS             complement(5275..6132)
                     /locus_tag="VFQ23_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9907000.1"
                     /translation="MALFSGFSSNGVPYAKLSDSGPALMVLTGSELEHQPPTWTAQQG
                     FLVGLKQLTKNYSIYLMSRKPNLPKGYTAQDMSDDFAEMIRNDIGMPVHLMGISSGGS
                     SAMHLAVDHPDLVNKLVLAMTGYRLNENGKQVAEIWRDLALQEDWPALYQRMGIDVAE
                     GSTPEWVTRLMMRLFGRLLLGTPKSGSDFAIVLDSDINLAVKDKLPSIRQLTLVIGGE
                     KDPFYGAANIRETARLIPNAELCLLKDGGHAVVKSQTKAFEAAILTFLQKENETITNH
                     AEANIPEPA"
     gene            6256..7281
                     /locus_tag="VFQ23_10180"
     CDS             6256..7281
                     /locus_tag="VFQ23_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9907001.1"
                     /translation="MKSNRLYMIYAILLALLIACQLSTLTLTPTSIPTLPAALSPTST
                     STFVPTIALTPTSTNTLSPTETPLPAIRRVIILSIDGLRPDAIALAPMPNLMALMQTS
                     AFSLSAQTVLPSVTLVSHSSMLVGVCPSKHGVDWNDYIPENGFAIGTDLFDLAHAAGL
                     QTVMYVGKEKLQQVTEPASLDKFVYVNDRDLVLMQRLIAEFPQDFDVLFIHLPLVDGM
                     GHVYGWLSPEQLSVAIRADEALGQLLAELDARGLRGETLIIISADHGGHSTTHGSSLP
                     EDMTIPWIASGPGIQPTQLTTQVHTMDTAATAAFALGLPLPPEWDGVPVYEAFGLPVS
                     RQSVACQ"
     gene            7676..>8363
                     /locus_tag="VFQ23_10185"
     CDS             7676..>8363
                     /locus_tag="VFQ23_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907002.1"
                     /translation="MNRKLTSLNLLARLGLLVVLVAVLAACGGESLDPSSGYGPNPVL
                     PTPQQSIIPVLNPPNAIGWDNGDAPLAAEGLTVTAFASGLDHPRWLYVLPNGDVLVAE
                     TNAPERPDDARGIKGFFFKFFQKRAGGGVPSANRITLLRDEDGDGVAETRSTFIDGLN
                     SPFGMALVGSTFYVANTDAVVSFPYTEGQTEITAEPTKVADLPAGRINHHWTKGLIAS
                     PDGSKLYASIG"
BASE COUNT         2032 a         2078 c         2111 g         2142 t
ORIGIN      
        1 tgcctggagt aaatatgcgg gcattttgtt cagtgccctg attatcatca gcgcaatctt
       61 attcaactat gaactttatc catctgaatg ctgcatcctt gtaggagccg atgatgtggt
      121 ggcaatcgat tggatggata aaaatctgcc gcccgacgca tcggtggcga tttctgccgt
      181 ggaactgatc gtcctggcat ccgattcgtt tcaggggtat gtcggcggcg acgcagggat
      241 ctggatcaca cctttaacca gtcagccaac gatccctttt ctcaatcact ctgatttcag
      301 ccaggcaaca acacttcaca ctctctgtga actgaatgcg cattacttat atatcggtga
      361 aacaggtcag gtttttgatg atgcacagat aacagctcaa ccgcaatggt acaaggccct
      421 gctatcgatg ccaaaatcaa aagtctatca gatcattggg tgtacataaa aaatgcgctc
      481 ccactaagta tgtggagcgc atttttgttt ttgttattag gaagaatatc aggcttttcc
      541 acccgtttgg tccaatttcc tcttacccac tctattgtgg cgcaactggg cgcaacgcca
      601 gctcgatatc ctcgcgcagg tcgaggaaaa attgaagtgg tagtcggacg ggtgtaaacg
      661 ttattccgct atccatctgg tcaatgggaa cataatataa ctgggttatg ctgttttccc
      721 cgcggcgtac gtcctgaaac acaaacagga tgtgagtccc atctggactg aagattgcac
      781 tgttatagca gcaaacccca tcgatcgggt tgaccttacg ctctgtcatg gtggacatat
      841 cgtaaaggta tagttcacca taggccgtgt tacgtttgaa actgttaaat aggaatcgct
      901 ggttcccatc ccagtggtag gatggcaaca atggatatcg ttcataacct tcgggcataa
      961 aatgcttggc agggaattcg tccatgatca atgggtctgc tgcctgacag cgccgcgtat
     1021 caatatccag gacgcggatg gtatcgccca ggcgttgtcc tactacactt tcgtaggtga
     1081 tcgcgagttc ctgtccggtt gccgaccaaa gcgcgccttt taccgagaca tcggagtaat
     1141 caaggcagag atcctccagc ccctgtagtt caaacgtcgt tgccactgtg gaaagcgttt
     1201 gtttgtcgaa tcgtaggaca atgagacgat tcccaatact aatcgcaacg tgggtgccat
     1261 ctggtgagac acgaaagcca tcgaagtctt cttcattaaa gcatgcgagt ttttcagaag
     1321 ctacctcagg aacttccacg ttgatcgtgt aaaaacaatt gccttcccca tagagaagtt
     1381 catttccatc tggaagccat tgcaggtcaa acttaggtac attcgtgtta gtcagttgca
     1441 aaatattcct gccatccata tccatcatga aaatatcgtt gttggcactt agcgcgatct
     1501 tatctgcgcc gccaatggtg agtatagggt tgactgtcgg cgtaacttca ttgacagctg
     1561 tagcggttga tgtgggaacg atgaattctg tgggcgtgct tgttggagga gaagcggtga
     1621 aggtcgaagt tgcagtttca gaagtcgggt tgaacgacgc agcaaaacgt ggatatcccc
     1681 aaagcgcggc aagtgtcaat atgatgaatc cgccaaacat ccaaaagcgc gagatcggct
     1741 ggcgtttgtg tgccccggtg gagggaggtg gcgtcagcac cgcgtcattg atctgaaccg
     1801 gttttggaat gggtgcgaca agatgggcat taccggcaat cgttgcagaa aagtcattgg
     1861 caaactcaac agcagagcta tagcgttgat ctcgatcctt tgccatgact ttttcgatga
     1921 cggtttcgat gccggtcggc aggtctgggt tgaccctcag aatgtgtggc accggctcgg
     1981 ctgcatgttt gaatgccatc cctaacgggg tagttgcttc atagggagtt ttaccgctta
     2041 acatttcgaa caggatcacg cccagtgaat agatatcact gcgaccatcc acttcctcgc
     2101 cctgtgcctg ctcgggactc atataagtgg gggtaccaat aatgccggag tgagtcagtt
     2161 tgatggaagc ccgggagatt ttggcgatac caaaatcgga aataaatgga ttgttgtatt
     2221 catcgaacag gatgttgccc ggtttcaaat cgcgatggat cacatcttta ctatgtgcat
     2281 agtccagcgc ggcggccacg cgttgaataa tatacgcaat ctcactgaag gtcatcgact
     2341 tgttctggat gcggtctgaa agcgaaccgc cagacatata ccgcatcacg aaaaataact
     2401 ggtccttatc gcggcctaca tcatagaccg ggacaattgc agcatgttcg agcttggcaa
     2461 taatctttgt ctcgcgctcg aagcgttcac ggatctgaga gttggcaagc aattcctgtt
     2521 tgaggatttt gagggcgact tcgcgttcga acagcggatc gtatgcgcga taaactgtag
     2581 ccattccgcc ctggcccaat tcctccctga tctcatatct gctaaattta tcagcttgca
     2641 tgtcgcaagt ataacgagcg atgtgaattg caagcagtac atttgaatta cagaaattcg
     2701 aggccctatg gtgagcattc ccttctcttt ataagttacg gtatcctgtt aatagaatcg
     2761 taacagtctc gtaagagttt ccaaaggtga tgctcatatt actcttttag actggggatg
     2821 tagcgcggat cgcgatcctc tcgctgtttg ttgaagtgaa cgatacgtta aaagttaata
     2881 aaggagttta aaatgaaacg aaaactaagt atgcttttcg tcgtattgct gctcgcgctt
     2941 gtcatggtga ttcctgcttc ggcaggacgc gggaatgaga ccccgggtgt tgtctacgtc
     3001 acgagccagg gtttgtatta cgatacgttt gtggtgaaag acccattacc ggccaatggc
     3061 cgtttccagc aaattgtaaa tgggacgact gagtttggac ccggcgaccc aggttacctg
     3121 ggcggacgct ggtgggacga taccaacatg aacggcatta tggatgccac cgatcactac
     3181 ttcctgtgtc cgctactcgg accgggacgc gaaaccccat agtgaatctg aaaccgctgc
     3241 tgtaaacatc acagacgcta taaaactccg agacgtggat ccgtctcgga gttctttttt
     3301 cttttcttct tcaatgtcat ctactctttg aaaaaattgg taatggatgc gagtcgaccg
     3361 agcgtatacc ctgtcgaagt catgtgactt tcggatgttt atttttctac attcgccata
     3421 ctcacggtgt tttttgttaa ctttgctgtg taaagagcct gatctgcatt tgttaggagc
     3481 tcatcgattt gattggctga agtgccacgc gcgactccaa tgctgatcgt gatattaaaa
     3541 gttatatctc cctgtttgta ttctgaattg gcaatttctc gccgcaatct ctccgctagt
     3601 gcctgtgcat cgggggtgct tggcagaata ataagaaatt cttcaccacc ccagcgaccc
     3661 gcccaatcgt gcttgcggat catggactga aagatgcctg ccgttttgac caagacatag
     3721 tcacccatgg catgacccca gcggtcattc acctgcttga aattatcgag atcgatcagt
     3781 aatatagaaa attcactatg atctctctgt gagcgagaaa gttccctctc gaggaaggtt
     3841 tgcgtcgcgc gccgatttgg gatgcgcgtc aggacgtcga tggtggcgac ttccatgagg
     3901 tcgttccgca gccgctggct caccatcagg ataaaaccca tcgaccagaa aaaactgagt
     3961 gcaaaggcga aaagataggt ggcagattga acgggagaca ggccggaaac ctccagtgga
     4021 ggttgcagga gaatcgcgat ggtgcggatg acgaggaaca gcccatagac aagaaacgag
     4081 actaacatca ggtttgcact aagcttgagt gctgtcttct gtatctgcca gagctgatgg
     4141 atcaggagcg aaaccatggc gatcgacccg agcgaaataa cgaggatacg ctttccgact
     4201 tcatcgaccc cataggtaaa gtacaacaga aaagccaata cacttacaaa gattccagtc
     4261 aaagctatct ttttgtaagc gaagccagtg aattgactga gcgctatgta aaacatagct
     4321 gtccccgtca ggatcaacat attggatact atgattgtaa ggaaaatcgg cagctgctcg
     4381 gcgaacaggc ggatcatcag tcccaccgct gcacacaagt ttgccagcag cgctgcaatc
     4441 acccctcgat attgttttag ctcccgaatc aaataggcct gtgcgccaat gacggtcgcc
     4501 tggacgaaga acgtcatggc gacaaagagc gcaatggtct ccacttcaaa ctggaaaagt
     4561 attgacatag aaggccctag ccgatacatc ataacgtatc cccataggca gggatagggg
     4621 aaaattagta aaggtgcgtg ttcaggttaa cgtctctaat tatttcattc ggcagatctg
     4681 gacgtcagcc cgttcttcaa tacgtcaatt acctgatcga acacgtcgct cacttcggga
     4741 cgggcgaccc aatccccgtt tcttttgcca aatttcgatt gcaggaacga gcccatctca
     4801 ctcataagtc cctgtaccat aaagctgacc acggcggggt cgaggtgggg atcaagattc
     4861 cctgaagcct gcgcccgcgc caccaatccc tgaaagtgct cttcgcgcac cttctttgct
     4921 ttttccagaa tcgagtcacg aaagggcagc ggcgaggtgt aggcgcggcg cgccagcttt
     4981 gagtggatcg gaaacctggc ggaggctcgc accgcggccg acatctgcca gcgcagtgct
     5041 tcaaagaaat cagcatttgg gtctggtgaa ggcgattgct caacgaccgc catcatctcc
     5101 tgattggata catccagcag gtgcatgaat agatcctgtt tgtcttcgaa atactgatag
     5161 acgctcccct ttgcaatccc tgctttttcg acgatcttcg aaacggaggc ggtgttgtaa
     5221 tcgttgttag cgaattcatc cagcgcgatc tcgaggaagg ccctgcgctt ttcctcaggc
     5281 aggttcagga atgtttgctt cggcatggtt tgttatcgtc tcattttctt tttgaaggaa
     5341 ggttagaatt gctgcctcaa atgctttcgt ctgcgatttg acgaccgcgt ggccgccatc
     5401 tttgagcagg cacagttccg cgttgggaat cagccgtgca gtctcgcgga tgttagcggc
     5461 accatagaac ggatctttct ctccaccgat caccagcgtg agttggcgaa tggatggcag
     5521 cttatcttta accgccaggt tgatatcgga atcaagcaca atggcaaaat ctgatccgct
     5581 cttcggtgtg ccaagcagca gcctgccaaa caaacgcatc atcagtcggg tgacccattc
     5641 cggtgtggaa ccttccgcta catcgatgcc catgcgttga tacagtgcag gccagtcttc
     5701 ctgtaacgcc aggtcacgcc agatttcagc cacctgtttc ccgttctcgt tgagacggta
     5761 gccggtcatg gcaagaacaa gtttatttac cagatccggg tggtccactg ccaaatgcat
     5821 cgcgctggat ccgccactgg atatgcccat gagatgcact ggcatgccga tatcgttgcg
     5881 gatcatctct gcaaagtcgt cgctcatatc ctgtgctgta taccctttgg gaaggtttgg
     5941 cttacggctc atcaaataga tgctgtagtt tttcgttagt tgtttaagac cgaccaggaa
     6001 tccctgctgc gccgtccagg tgggtggctg gtgttcaagt tcagaacccg taagaaccat
     6061 tagcgccgga cccgaatcgc tgagttttgc atagggaacg ccgttggatg agaagccgga
     6121 aaagagtgcc atttctacct ccaggtcgag ttgtgactgg tcagtcatat gactgaccag
     6181 tcacattata agcatatcgt atgaaatgtc aagaggcaga ctcgtggatt acatgtcgta
     6241 ttacaatcct gttcaatgaa atcaaaccgt ctttacatga tctatgccat cctgctggcg
     6301 cttttgatcg catgccagtt atctactctg acgcttaccc ccacctcgat accgacatta
     6361 cctgccgcgc ttagtcccac ctctacatct accttcgttc ctaccattgc attaactcca
     6421 acgtcaacaa atacgctgtc accgacagaa acaccgttac ctgcaattcg ccgagttatc
     6481 atcctcagca tcgacggctt gcgcccggat gctatcgcat tggcacccat gccgaatctg
     6541 atggctttga tgcaaacctc tgcattttca ctttcggcac aaacggtcct tccaagtgtc
     6601 acactggttt cgcattcttc catgctggtt ggggtgtgcc catccaaaca tggtgtggat
     6661 tggaacgatt acatccctga aaatggattc gccatcggca ccgatctgtt tgaccttgcg
     6721 cacgcggcag gattgcagac tgtgatgtat gtgggcaagg aaaagcttca gcaagtcact
     6781 gaacctgcca gcctggacaa atttgtttat gtcaacgatc gcgacctcgt tctcatgcag
     6841 cgactcatcg cagaattccc acaggatttt gacgtgcttt ttattcacct ccctctcgtg
     6901 gatggaatgg gacacgtata cggctggtta tcgccggaac aactgagcgt ggcgattcgc
     6961 gccgacgaag cgctgggtca gctcctggct gagttggatg cgcgaggtct gcgcggcgaa
     7021 acgctgatta tcatttcagc cgatcatgga ggtcatagta caacgcacgg gtccagcctg
     7081 cccgaagata tgacgattcc atggatcgcc tcaggaccag gcatacagcc gacacaactc
     7141 acaacgcaag tgcacacgat ggacacggcc gctacggctg cgtttgcgct gggcctgccg
     7201 ctccctcccg aatgggatgg cgtacctgtg tatgaggcat tcggcttgcc tgtctcaagg
     7261 caatctgttg cctgtcaata agcgcagcga atgcatacca caatggagca gctacgaata
     7321 ggcgatctct tcaatcttca atataaaaac ataaatcaat aagcttaaga aaagctcata
     7381 aaaatcgttg agtgctcgtt tgaatggagt agggattgcg tccagcatgg tttggcggga
     7441 cgtgctaaag ttgagttctc ttcttctcct cctgctagat tgaaagagcc gggcttggac
     7501 tcccggctct ttcaatttaa tccaatggaa tcaatcatcc gccgatgtga tacagcaaca
     7561 aactctgagc ctgttcgtta ttacatttaa atgaaaatgt attagcactg cgactttgtt
     7621 gatttgcagg ggatttcata ggatttgtgg tgctcgtttt taccggagaa ggaatatgaa
     7681 cagaaaattg acttctctca atctcctcgc gagattgggc ttgcttgtcg tgcttgtggc
     7741 cgtcctggcg gcctgcggag gagagtccct ggatccctcc agtggatatg gaccgaatcc
     7801 agttctaccc acccctcagc aatcgatcat ccctgtgctg aatccgccga atgccatcgg
     7861 ctgggataat ggtgatgcgc cccttgcagc ggaaggactc actgtgaccg ccttcgccag
     7921 cggattggat cacccgcgct ggctgtatgt gctgccgaat ggggatgtgc tcgtggcaga
     7981 aaccaatgcg cctgagcgtc ccgatgacgc cagggggatc aaagggttct tcttcaaatt
     8041 ttttcagaag agggccggcg gcggcgtccc cagtgccaat cgtattacgc tgttgagaga
     8101 tgaggatgga gacggcgttg cagagacacg atcgactttc attgatggct tgaattcacc
     8161 ctttggaatg gcactggtag gaagtacatt ttatgtcgcc aacacagatg cggttgtgtc
     8221 cttcccctat acagaaggac agaccgagat cactgctgaa ccgaccaagg tcgcagatct
     8281 gccggccggc cgcatcaatc atcattggac caaaggattg atcgccagcc cggatggatc
     8341 gaagctctat gcttccattg ggt
//
LOCUS       DASTJP010000340         4213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1275527, whole genome shotgun sequence.
ACCESSION   DASTJP010000340 DASTJP010000000
VERSION     DASTJP010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275527"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VFQ23_10190"
     CDS             <1..1010
                     /locus_tag="VFQ23_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907003.1"
                     /translation="KNQNQTIYTGDISAEQIDPTREYSTDNQEIIFSKDQHAIWADLF
                     AGIHRPYLLEHICQQFIHGLKFLQLDPLHIPTVAHLNEQIQTRTGWRIERTVVRYTLA
                     DDWYAKFAQRTFLITDYLRTRDQMEFTPEPDMFHDIFGHLPYLTQEFYARIEDKFAPA
                     YLKATQEEREVIKRLAWYSTEFGLIMEDNRIKVFGAGTISGRAELANTIMEFYRLSRD
                     KVIDYSGDVFEQLQDHYLTHKENINRIVEGVNQLHRQGQMSSQDKGWNVVHALYQDLG
                     IPHEGYLGGEVILAPFDIETIARIPKTVYAFNPMFFVCESFEKMDELLESYLGPISQR
                     N"
     gene            1138..1797
                     /locus_tag="VFQ23_10195"
     CDS             1138..1797
                     /locus_tag="VFQ23_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907004.1"
                     /translation="MPMSILDSVFAGIDVSSGRKPITYAVLDDGLNVVLLEKCDMSYV
                     IAHLEQHRKVLLGVNLRPGAKSANSLRTQNAYEEIRKKIFQSGIKPYLSNNAPKQWVE
                     TNPDECFQSLSGQVPGSRRTLGGLIQRALILYDEGLQLRDPMEFFEEITRHHLMSGIL
                     PMEMLYSASELDALAAAYISWLLVNKPVQVDLTKNRGEGMILIPREDKNWWRKKPNTN
                     N"
     gene            complement(1814..2353)
                     /locus_tag="VFQ23_10200"
     CDS             complement(1814..2353)
                     /locus_tag="VFQ23_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HET9907005.1"
                     /translation="MNFSLTPFSLKDSPQVTISGTVKQQNNNISISYIVAGNIEEIYF
                     PPALANPTRKDDLWKTTCFEFFIAAKDSPGYWEFNLSPSGEWNVYIMDAYRQVNMREE
                     TRVTKLEIRVQKEEKCFVLEHELNLDSIMLEETSAQVGIASVIAANTGQLSYWALTHP
                     HAEADFHLRNSFVLEFPAS"
     gene            complement(2350..3447)
                     /locus_tag="VFQ23_10205"
     CDS             complement(2350..3447)
                     /locus_tag="VFQ23_10205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9907006.1"
                     /translation="MSNFSAIAQQFESSGQVIEVREFGNGNINDTYLVTTDAADEKYF
                     VLQRINTNVFRQPKLIMQNMRAFTEHMYRRASEEGHPWKMPRVIATQDGQDFFLDSEG
                     NFWRAISFIHGAQSFSTVQNMDHAREVGHALGMFHYLINDLAIDRLADTLEGFHIVPR
                     YLQSFDLALAENRFKTNAEVTYCLNFVDTRRDWSHVLENARKQGRLHLRPVHGDPKVD
                     NVMIEKSTGIAISLVDLDTVKPGLIHYDIGDCIRSACNPLGEETEDWEAVHFEPQIGA
                     AILEGYLAQAQRFLTPTDYVYLFDSIRLLTFELGLRFITDYMAGNTYFKVKDADHNLR
                     RALVQFKLTESIEAHEAEIHNIIQSVVSRTA"
     gene            3538..>4213
                     /locus_tag="VFQ23_10210"
     CDS             3538..>4213
                     /locus_tag="VFQ23_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET9907007.1"
                     /translation="MTDDLQLRAINTIRFLAADAVQQANSGHPGLPMGAAAMAYTVWT
                     RHLRHNPHNPKWPGRDRFILSGGHGSMLLYSLLHLTGYDLPMEEIKNFRQFDSKTPGH
                     PEYGLTPGVEVTTGPLGQGFGQGVGMAIAATQLAATFNQPGHELIQSHIYGIVTDGDL
                     MEGVASEAASLAGHLGLGRIIYLYDDNHISIDGSTDLAFTEDRADRFRAYHWHVQTVE
                     DGNDVEA"
BASE COUNT         1084 a          964 c         1028 g         1137 t
ORIGIN      
        1 ataagaatca aaaccagaca atttacacgg gggatatttc agcagaacag atcgacccca
       61 cccgggaata ttcaacggat aaccaggaaa ttatcttttc taaggaccag catgcgattt
      121 gggcggatct ctttgctggt atccatcgtc cgtatttgct cgagcatatc tgtcagcaat
      181 ttattcatgg actaaaattc ttgcaactcg accctcttca tatccctacc gtggctcact
      241 tgaacgagca gatccaaact cggaccgggt ggcgaatcga acggaccgtt gttcgttata
      301 cactggcaga tgactggtat gccaaatttg cgcaacgcac tttcctgata accgattatc
      361 tgcgcacccg tgaccagatg gagtttaccc ctgagcccga catgttccat gatatttttg
      421 ggcatctgcc gtacttaact caggaattct atgcccgaat tgaggataag tttgccccag
      481 cctatctaaa ggcaactcag gaagaacgag aagttatcaa acgtctggca tggtacagca
      541 ccgagtttgg cttgatcatg gaggacaacc gtattaaggt gtttggggca gggacgattt
      601 caggcagagc cgaactcgcc aatacaatta tggagttcta tcgcctcagc cgcgataagg
      661 tgattgatta cagcggcgat gtctttgagc agcttcagga tcattactta acccacaaag
      721 agaatatcaa ccggatcgtg gaaggcgtca atcaactgca ccgtcaggga cagatgtctt
      781 cgcaggataa aggttggaat gtcgtccacg ccttgtatca ggatcttgga attccgcatg
      841 aagggtattt gggaggtgag gtgatccttg ctccctttga tatcgagacc atcgcccgga
      901 tccccaaaac cgtgtatgcg ttcaacccga tgttctttgt ctgtgagtct tttgaaaaaa
      961 tggatgagtt gcttgaatct tatctggggc caatttcgca acgaaattga agataattct
     1021 cttcatggac gctagaggat tactttactt gaatactttc tagggtcaga atattaccgg
     1081 ccgaccgcct ctttgcctca cactgacgag aggcggtcaa cattttgcac tcagggtatg
     1141 cctatgtcaa ttctggattc agtttttgcg ggtatcgatg tttcatcggg acgcaaacca
     1201 atcacgtatg ccgtgctaga cgatggtctg aacgtagttc tgcttgaaaa atgtgatatg
     1261 tcctatgtga ttgcgcatct tgagcaacac aggaaggtac tattaggcgt gaacctccga
     1321 cctggcgcga aatctgcaaa ttctctgcgc acacaaaatg cctatgagga gatcagaaag
     1381 aaaatatttc agtctggaat taagccctat ttgtccaata acgcgccaaa acaatgggtg
     1441 gaaacaaacc cggatgagtg tttccaatca ctttccgggc aggtacccgg atcccggcgt
     1501 acgctgggtg gattaattca gcgtgcgctg attttgtacg atgaagggct gcaactcagg
     1561 gatcccatgg aattctttga ggagatcaca aggcaccact taatgtcagg aattctgcct
     1621 atggaaatgc tttattccgc ttcagaactg gatgccctgg cagcagctta tatctcgtgg
     1681 ttgttagtca ataaaccagt tcaggtagac cttacgaaaa atcggggaga aggaatgatt
     1741 ttgataccca gggaggataa aaattggtgg agaaagaaac caaatactaa caactaaatc
     1801 tttgtttttt tcattatgat gcggggaact cgaggacaaa gctgtttcgc aagtgaaaat
     1861 ctgcttcggc gtgagggtgt gtcaatgccc agtaggatag ttgtccggtg tttgctgcta
     1921 ttacacttgc gatgcctact tgtgcagagg tctcttctaa cataatagaa tccaggttaa
     1981 gctcatgttc aagcacaaag cacttctcct ccttttggac cctaatctct agctttgtga
     2041 cccgcgtttc ctccctcatg ttgacctgcc ggtaggcatc cattatatag acgttccatt
     2101 ctccagaagg cgatagatta aattcccaat atcctggtga gtctttggct gcaatgaaga
     2161 actcaaaaca agtcgttttc caaaggtcat ctttgcgggt tgggttggct aatgcgggtg
     2221 gaaaataaat ctcctcgata tttcccgcaa caatatagga aatcgaaata ttattgttct
     2281 gttgcttgac cgttccactg atcgttacct gaggagaatc cttaagagag aacggagtta
     2341 atgaaaagtt catgctgtgc gtgaaaccac tgactgaata atgttatgaa tttctgcttc
     2401 gtgtgcttct atggattcgg tcaatttgaa ttgtacaagt gcgcggcgta aattatggtc
     2461 ggcatctttt actttgaaat atgtattgcc tgccatgtaa tcagtaataa agcgcaagcc
     2521 aagctcaaac gtcagtaacc gaattgagtc gaacagatac acataatcgg ttggagtcag
     2581 gaaccgttgc gcctgagcca ggtagccttc cagaatcgcc gcgccaattt gaggttcaaa
     2641 atgaactgcc tcccagtcct cagtttcctc gccaagcggg ttgcatgccg aacggatgca
     2701 atccccaatg tcgtaatgga tgagcccggg ttttacggta tccaaatcca cgaggctgat
     2761 tgcgatgcca gtggatttct cgatcatcac gttatcaacc ttcgggtcgc catgcaccgg
     2821 gcgcaaatga agcctgcctt gtttgcgggc gttttcaagg acgtgtgacc aatcgcgcct
     2881 ggtatctaca aagttcaggc aatacgttac ctctgcattt gttttgaatc gattttctgc
     2941 cagagccaag tcaaaactct gaagataacg gggcacaata tgaaagcctt cgagcgtgtc
     3001 ggcgagtcta tcaatggcaa gatcgttgat aagatagtgg aacatcccaa gggcgtgacc
     3061 tacttcacgg gcgtgatcca tgttctgaac agtgctgaac gattgggccc cgtggatgaa
     3121 gctgatcgca cgccagaagt tcccctcgga atccaggaag aaatcctggc cgtcctgtgt
     3181 ggcgatcacg cgtggcattt tccagggatg tccctcctcg cttgcgcgtc gatacatatg
     3241 ttctgtgaag gcgcgcatat tttgcatgat cagcttcggt tgacgaaaga catttgtgtt
     3301 gattcgttgc aatacgaaat atttttcatc cgctgcatca gtggttacga gatatgtgtc
     3361 gttgatattt ccgttcccaa attcgcgaac ttcaatgact tgaccagatg attcaaattg
     3421 ttgtgcaatg gctgaaaagt tggacacatg aatgctccca gtgattgata atgacttatg
     3481 attatatcct tgatggtacg atggtaaaat gggttatcct tttcctgtag gttacatatg
     3541 acagacgacc ttcaacttcg tgcgattaac accatccgct tcttagccgc ggatgctgtt
     3601 cagcaagcca attcaggtca ccctgggctt cccatgggtg ccgctgccat ggcttatacc
     3661 gtttggacga gacacttgcg ccacaatccg cataatccca aatggccggg gcgtgatcgt
     3721 ttcattctct ccggcggaca tggttcgatg ctgctatatt ccctgttgca tctgaccggt
     3781 tacgatcttc caatggaaga aatcaaaaac tttcgtcaat ttgacagcaa gacgccgggt
     3841 catccggaat atggattgac cccgggtgtg gaagtgacga ccggtccact ggggcaggga
     3901 tttgggcagg gggtaggtat ggcaattgcg gcaactcaat tggccgccac tttcaaccaa
     3961 ccggggcatg agctcatcca atcacatatc tatggaatcg ttaccgatgg tgatctgatg
     4021 gagggcgtcg cttctgaagc tgcctcctta gccgggcatt tggggctcgg ccggatcatc
     4081 tatctttatg atgataacca tatctcgatt gatggctcta cggatttggc gtttacagaa
     4141 gatcgagcgg atcgattcag agcatatcat tggcatgtac aaactgtaga agacggaaac
     4201 gatgtcgaag cca
//
LOCUS       DASTJP010000341         6163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1061998, whole genome shotgun sequence.
ACCESSION   DASTJP010000341 DASTJP010000000
VERSION     DASTJP010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061998"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VFQ23_10215"
     CDS             complement(<1..1294)
                     /locus_tag="VFQ23_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907008.1"
                     /translation="MKKHPVLCLLFTFIIGNCTPAPASPTPYPTATVTPTRTPLPTQT
                     AIPTATPYPSLQSEGPYLIFTYDNKNFTIMDADGNGRKQFQLPNDGYIRDLEKAVSPD
                     GKWLAYFTGLTDDPYDISLNLFNLETEGNLKIANLIAKNFPQNLEPAIRILKFAGCIN
                     NEECKLSLFKLSLFDGIKSLEWSPDGKQLAFAAQIDGPSSDIYSFGTESKTTQRLTME
                     PENIDEIIWSPNGEKIIYKASIEGSDFYPSTIWHLADLTIAMPQNSKSISEEKPWRGL
                     GWFNEDLYFISTAIDSPVYIDTGYINIDSGEIKIIWPHITESLILDSKNNRIILSFMP
                     YGVNDPEITAGTYLLSFDGSIRKISDEIHIFFYKQGFSGSYFAVGQSGLVRISSDGNI
                     TYVAENTPHIKSISPSPNNKWTFVAREEVLELFSENLDL"
     gene            1468..1821
                     /locus_tag="VFQ23_10220"
     CDS             1468..1821
                     /locus_tag="VFQ23_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9907009.1"
                     /translation="MLEYVAIALMIVLATLVALIAIGLGTLFGPRKPSQEKSMPYESG
                     MPPIGEGTRRVPVRFYLIAVLFILFDIEVIFFLPWAVVFRQLGLFGLIEMFIFIVILL
                     VGYVYAWKKGALEWE"
     gene            1812..2294
                     /locus_tag="VFQ23_10225"
     CDS             1812..2294
                     /locus_tag="VFQ23_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET9907010.1"
                     /translation="MGIEQKLGNMGVATTTLETAVNWARTNAMWPMLFGLACCAIEMM
                     AAQASHYDMSRFGMELMRASPRQSDLMIVAGRLSRKMAPVLRRLYDQMPEPKWVISMG
                     DCASCSGVFNNYALVQGVDEIVPVDVYVAGCPPRPEALIHGVVTLYEKVKGEKFVAST
                     "
     gene            2316..2831
                     /locus_tag="VFQ23_10230"
     CDS             2316..2831
                     /locus_tag="VFQ23_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET9907011.1"
                     /translation="MDKKLEPIVQALQESYATSIEEFREEVHVFVKAENIVSALTLLR
                     DKYEFELLSALTASDYLNLQQSPRFHVIYQLSSIARNLTLQLRVPLEGDNPKLPTATG
                     VYDVANWRERELLDMFGIEIEGHPDPRRILMPEGTEGHPLRKDFPLGYEEPQFTFNFE
                     EIDLKKPYAKE"
     gene            2850..4079
                     /gene="nuoD"
                     /locus_tag="VFQ23_10235"
     CDS             2850..4079
                     /gene="nuoD"
                     /locus_tag="VFQ23_10235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HET9907012.1"
                     /translation="MAQLEEANIDLYKHLVSERALSGETMLLNMGPQHPSTHGVLRLL
                     LELDGETVINCIPDIGYLHTGIEKNMEAKTYLKAEVMSDRLDYMNTMGNNLAYVMAVE
                     KLVGLDVPPRAQAIRVILTELQRIASHLVFLGAFGLDLAAMSVFLYCFRERESILDIA
                     ELCSGQRMMTTYFRPGGLWRDVPVEFETAVRDFLKIFPKRVDEYEGLLTKNPILLDRT
                     VGIGKLDVATALQHGVTGPPLRASGLSWDLRKARPYSGYEQYDFNIPTRTEGDTFARY
                     IVRVEEFRESMKIIEQALNKLPMGPINSDNRKFVPPPRSEIGVSMEALIHHFKLWTEG
                     FPAPRASVYSAVESPRGELGVYLEGDGGPKPQRVHWRTPSFDNLSALEKMTRGHLVAD
                     LVAILASIDIVLGDIDR"
     gene            4076..4582
                     /locus_tag="VFQ23_10240"
     CDS             4076..4582
                     /locus_tag="VFQ23_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET9907013.1"
                     /translation="MSLEEKYPNEVQQILSKYPPEGKRSAVMPLLYLAQREESYVSRA
                     AMRDIAKILDITETDVASIVRFYTLYHDKKEGKFHMQVCTDLPCALRGAEEFMDDLCE
                     GLGIKVGETTPDGFLTLEAVTCLAACDKAPMFQTQGPDGIKYHENMNVDKAMELISIL
                     KRSNVGMK"
     gene            4582..5859
                     /gene="nuoF"
                     /locus_tag="VFQ23_10245"
     CDS             4582..5859
                     /gene="nuoF"
                     /locus_tag="VFQ23_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET9907014.1"
                     /translation="MAYILLRHRDIPNLNQFDVYKENGGFAAFENAVTKMKPNEVTDV
                     VKNSGLRGRGGAGFPTGMKWSFIDNKSWPHYVVANADESEPGTFKDREIMEGNPFQFL
                     EGLALASYAVGANAAYVYLRGEFWELTAALDQKIAEMEKAGYLGEKLFGTDYSLRIYT
                     HLGAGAYICGEETALLESLEGKRGQPRVRPPFPPAVGLYGKPTIINNVETFTNVPMIL
                     ANGAEWYKSIGTADSAGVKVFSLSGRVRKPGNYEVPFGTTFRDLIYKYGGGVQDSRTV
                     KAIMPAGASSSMIPVDDKVLDTPIDYASVRTLGADLGSASVIVIDDTVSLKWLINKTV
                     HFFRHESCGKCTPCREGTYWMSHLTEDLVHNNGSVDLLLNVAKQIQGKCLCALGEFST
                     MAVVTGIERFRKEFETTTDTPVENYVETSNAHN"
     gene            5831..>6163
                     /locus_tag="VFQ23_10250"
     CDS             5831..>6163
                     /locus_tag="VFQ23_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9907015.1"
                     /translation="MSKQVTLTIDGKQVTVPEGLSVVDAAKMADIDIPVFCHHPKLDP
                     VGMCRMCLVEIGRPMRDRATGQFVMENGAPKIQFLPKLETGCTNKVEEGMVVLTQAQS
                     TKAKDGQRG"
BASE COUNT         1575 a         1381 c         1575 g         1632 t
ORIGIN      
        1 ctaaatccaa attttcggag aacaattcga gtacttcctc acgagctaca aatgtccatt
       61 tgttattggg agatgggctg atactcttga tatgtggggt gttttctgca acataggtta
      121 tgtttccatc ggatgaaata cgaaccaatc cgctttggcc aacagcaaaa taagatcccg
      181 aaaaaccctg cttataaaag aatatatgaa tttcatctga tattttcctg attgaaccat
      241 caaatgaaag taaataggtt cctgccgtga tttcagggtc gttaactcca taaggcataa
      301 aagacaagat aattctattg ttctttgaat ctaagattag gctctccgtt atatgtggcc
      361 aaataatctt tatttcccca gaatctatat ttatataccc agtgtctata taaacaggcg
      421 aatcaatcgc ggtggaaatg aagtacaaat cttcattaaa ccagccaaga cctcgccaag
      481 gtttttcttc acttatagac ttggagtttt gaggcatcgc aattgttaaa tctgctaagt
      541 gccatatggt gctcggatag aaatcgcttc cttctataga agctttgtat atgattttct
      601 ctccatttgg tgaccatata atttcatcaa tattctcagg ttccattgta agtctctgtg
      661 tggttttact ctcagtacca aagctataaa tgtcagaaga cgggccgtca atttgtgcgg
      721 cgaaggcaag ttgtttgcca tcaggagacc attcaagaga tttgattcca tcaaaaaggc
      781 taagcttaaa gagtgacaat ttgcattctt cgttgttgat gcagccagca aactttaata
      841 tccgaatagc aggttccaga ttttgaggaa aattttttgc aattagattt gcgattttta
      901 aattaccttc agtttcgaga ttgaataaat taagagaaat gtcgtaaggg tcatcagtta
      961 agcctgtaaa ataagcaagc cattttccat caggagaaac agccttttcc aaatccctta
     1021 tatatccatc atttggaagt tggaattgtt ttcggccgtt tccgtctgca tccatgatag
     1081 tgaaattctt gttgtcgtat gtgaatatca gatatggtcc ttcgctttga agtgaaggat
     1141 agggtgttgc ggtgggaatt gccgtttgtg ttggaagtgg ggtcctggtt ggggtaaccg
     1201 tagcagttgg atagggagta ggagatgcag gcgcgggcgt gcaattaccg ataatgaatg
     1261 taaatagcaa gcataaaaca gggtgttttt tcatattctc ttttgtaatt tgagtatagc
     1321 atttttcaaa atcccaaatc atatctatcg aatgactctt cctcttgtga aaagtggcac
     1381 tttcattatt cttatgttat actgtgccga acgttgtgca aggaagcaca aataccctct
     1441 gacaagggcg ttttgcgtga ggtcggaatg ctcgaatatg ttgcaatcgc attgatgatt
     1501 gttttggcga cattagtagc attgatcgcc attgggttgg ggacattatt tggaccacga
     1561 aaaccatctc aagaaaaaag catgccctat gaatctggca tgcctcccat cggcgaagga
     1621 acccgacgtg ttcctgtccg cttttatttg attgcagttt tattcatcct gttcgatatc
     1681 gaagttatat tttttctgcc gtgggcagtt gtatttcgcc aactgggttt atttggtttg
     1741 attgaaatgt tcatctttat tgtgatcctg cttgtaggat atgtgtatgc gtggaagaaa
     1801 ggagcccttg aatgggaata gaacaaaaac tcggcaatat gggcgtggcg actactacgc
     1861 tcgagactgc cgttaattgg gcacggacga atgcaatgtg gccgatgtta tttggattgg
     1921 cttgctgcgc gatcgaaatg atggcggcgc aggcctcgca ctatgacatg agtcgctttg
     1981 gcatggagtt aatgcgagcc agcccgcgtc aatcggactt gatgatcgtt gcgggacgcc
     2041 tgtcacggaa aatggcgccg gtgcttcgtc gcctgtacga tcagatgcct gagccaaaat
     2101 gggtgatctc gatgggagac tgcgcttcct gcagtggggt attcaacaac tacgccctcg
     2161 tgcagggtgt ggatgagatt gtccctgtgg atgtgtatgt agcaggctgc ccgccgcgcc
     2221 cggaggcgtt gatccacggt gtggtgaccc tgtatgaaaa ggtgaaaggc gagaagtttg
     2281 tagcctcgac ttaagtcgat gccacggggt tgtttatgga taagaaactt gaaccaatcg
     2341 tccaggcgtt gcaggaaagt tatgcgacat ccattgaaga gttccgcgag gaagtgcatg
     2401 tatttgtcaa agcagaaaat atcgtatctg cattgactct tctgcgcgac aaatacgaat
     2461 ttgaactgct ctctgcttta acagcttcag attatttgaa cttacaacaa tcgccgcgct
     2521 tccatgtgat ctatcagctc tcatcgattg cgagaaacct taccttgcag ttgagggttc
     2581 ctttagaagg tgacaatcca aaactcccga ccgcgactgg tgtttacgat gttgccaact
     2641 ggcgcgagcg cgagctcctc gatatgtttg gcattgagat tgaaggtcat cccgatccac
     2701 gtcgcatcct gatgccggaa ggcaccgagg gtcatccgct ccgtaaggat tttccacttg
     2761 gatacgaaga acctcaattc acttttaatt ttgaggaaat tgacttaaaa aagccgtatg
     2821 cgaaggaatg agtaaagtaa ggaatagata tggcacagct tgaagaagcc aatatagatt
     2881 tatacaagca tctggtatct gagcgtgcac ttagtggcga gaccatgtta ttgaacatgg
     2941 gacctcagca tccatccacg catggggttc ttcgcctgtt gcttgaactt gatggtgaga
     3001 cagttatcaa ttgcattccc gatatcggtt atctgcatac cggcattgaa aaaaacatgg
     3061 aagccaagac ctatctcaaa gccgaggtga tgagtgaccg tctggattac atgaacacga
     3121 tgggcaataa ccttgcctat gtgatggctg tggaaaaact ggtcggtctg gatgtccccc
     3181 cgcgtgcgca ggcgattcgt gtgattctca cggagctgca gcgtattgcg tcgcacctgg
     3241 tctttcttgg cgcgttcgga ctggatctcg cagcaatgtc tgttttcctg tattgtttcc
     3301 gcgagcgcga gtccattctg gatattgccg agctgtgttc cggccagcgc atgatgacca
     3361 cttactttcg accgggcgga ctctggcgcg atgtacctgt cgaatttgaa actgccgtgc
     3421 gcgattttct caagatattc cccaagcgtg tggatgaata cgaaggcctg ttgacgaaaa
     3481 atccgatcct gctggaccgt acagttggca tcggcaaact ggacgtagcc actgcccttc
     3541 agcatggcgt gaccgggcct cccctacgcg cctctggctt gagctgggat ctgcgcaaag
     3601 cgcgcccgta ttcgggctac gaacaatatg actttaacat ccccacgcga actgaaggcg
     3661 acacgttcgc gcgttacatt gtgcgtgttg aagaattccg cgaatcaatg aagatcatcg
     3721 agcaggcctt gaataaactg ccgatgggtc cgataaattc tgacaatcga aaatttgtcc
     3781 cgccaccgcg ttctgaaatt ggggtgagca tggaagcgtt gatccatcat ttcaaactat
     3841 ggacggaggg tttccctgca cctcgggctt ccgtttacag cgcagttgag tcaccccgcg
     3901 gtgaattggg tgtttatctg gaaggcgatg gaggacccaa gcctcaacgc gtccattggc
     3961 ggacaccttc ctttgataat ctctctgccc tcgagaaaat gacgcgcggg cacctcgttg
     4021 ccgacctggt tgcgatcctg gcttccatag acatcgtgct cggagatatt gaccgatgag
     4081 ccttgaagaa aaatatccga acgaagtaca acaaatccta tcgaaatatc ctccggaggg
     4141 gaagcgctcc gctgtgatgc cattgctgta tcttgctcaa cgtgaagaaa gttatgtgag
     4201 cagagcggcc atgcgcgaca tcgccaaaat tctggatatc acagaaacag atgtagcttc
     4261 aattgtcagg ttttatacgc tgtatcacga taaaaaagaa ggcaaatttc acatgcaggt
     4321 ttgtacagat ctgccttgtg ctctgcgtgg cgctgaagaa tttatggatg atctctgtga
     4381 aggcctggga atcaaagtcg gtgagacaac acccgatggg ttcttaaccc ttgaagcagt
     4441 gacctgcctg gctgcctgtg acaaggcacc catgttccag acacagggac cggatggaat
     4501 caaatatcac gagaatatga acgtggataa agcgatggaa ttgatttcta ttcttaaacg
     4561 atcgaatgtg gggatgaagt aatggcatat attctccttc gtcatcgcga tatcccaaat
     4621 ctcaatcagt ttgatgtcta taaagagaac ggcggctttg cggcgttcga aaacgcggtc
     4681 acaaagatga agccgaacga agtgaccgat gtagtaaaga actcaggtct gcgtggacgc
     4741 ggcggtgctg gcttcccgac cgggatgaaa tggtccttca ttgataacaa gagctggcct
     4801 cattacgtgg ttgcgaatgc ggacgagtct gagcctggca cgtttaaaga tcgcgagatc
     4861 atggaaggga atcccttcca gtttttagag ggtctcgcac ttgcatcgta tgcagtcgga
     4921 gcaaacgccg cgtatgttta tttgcgcggg gaattttggg aactgaccgc ggctctcgat
     4981 cagaagatcg cagaaatgga aaaagccggg tacctcggcg aaaagctctt cggtactgat
     5041 tattcactac gcatttacac ccatctgggt gccggtgcat atatttgtgg tgaagagacg
     5101 gccctgcttg agtcgctcga aggcaaacgc ggacagccac gggttcgccc gccgttccca
     5161 ccagccgttg gcttatatgg caagccgacc attatcaaca acgtggaaac attcaccaat
     5221 gtgccgatga tccttgccaa cggcgcagaa tggtacaaat ccatcggcac cgccgatagc
     5281 gccggcgtaa aggtattttc actctcgggc cgtgtgcgca agcccggcaa ctatgaagtg
     5341 ccgtttggta cgaccttccg cgatctgatt tataaatatg gcggcggtgt gcaggattca
     5401 cgtacggtca aagcaattat gcccgcgggt gcatcctctt ccatgatccc tgtggatgat
     5461 aaagtcctcg acacgccgat tgattacgcg tctgtccgta cgcttggcgc ggacctcggg
     5521 tctgcctcgg tgattgtcat tgatgacacg gtttcgttga aatggctgat caataaaacg
     5581 gttcatttct tcaggcatga atcctgtggc aaatgcactc cgtgccgtga gggcacgtat
     5641 tggatgtcgc atcttacaga agaccttgtt cacaataatg gaagcgtgga tctgctcctg
     5701 aacgtcgcca aacaaatcca ggggaaatgc ttgtgtgcgc tcggagaatt ttcaacgatg
     5761 gcagtggtca ccgggattga acgattcaga aaagaatttg agactacgac ggatacacca
     5821 gtagagaact atgtcgaaac aagtaacgct cacaattgat ggaaaacagg tcaccgtccc
     5881 ggaagggcta agcgtggtcg atgccgccaa gatggcggat atcgacattc ccgtcttctg
     5941 tcatcaccca aagctcgacc cggttggcat gtgccggatg tgccttgtgg agatcggtcg
     6001 cccaatgcgc gaccgcgcca ccggtcaatt cgtgatggag aacggcgcgc cgaagatcca
     6061 gttcctgccc aagcttgaaa cgggctgtac gaataaggtc gaagaaggca tggtcgtcct
     6121 cacgcaggca cagtcaacca aagccaagga tgggcagcga gga
//
LOCUS       DASTJP010000342         5876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_29116, whole genome shotgun sequence.
ACCESSION   DASTJP010000342 DASTJP010000000
VERSION     DASTJP010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29116"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..795)
                     /gene="rlmB"
                     /locus_tag="VFQ23_10255"
     CDS             complement(58..795)
                     /gene="rlmB"
                     /locus_tag="VFQ23_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET9907016.1"
                     /translation="MKEFIYSRNAVYETLRAKRRDIFKIELADNVQIKGRLAEIMSLT
                     SQRKIQVVKVPRARLDKIHDNNQGIMAEVGKYPYVDLLDILENARQKNEPTFVLLLDS
                     LNDPQNFGTLIRTAEAIGLHGVVIPLAHTVEVTPAVVNASSGASEHMLISRSNLAQAI
                     DTLKEENVWVVGLDQNGETIGAKTQRHLTGPVALVVGSEGEGIRQLTRAKCDIVLKLP
                     MRGRVESLNAAVAGSVALYLADLARNP"
     gene            complement(864..1208)
                     /locus_tag="VFQ23_10260"
     CDS             complement(864..1208)
                     /locus_tag="VFQ23_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907017.1"
                     /translation="MSYDVHQEKDYSNDGRSVYDAALKAVEKLGGKLLSSSPDDLRLE
                     VKFDKTILGQVLGDRTQLACVVQANGEGSKVVIDAYPLDAVGRKLMFGGRKGVTQTVI
                     TWFIAHLENNLK"
     gene            complement(1222..2151)
                     /gene="pfkB"
                     /locus_tag="VFQ23_10265"
     CDS             complement(1222..2151)
                     /gene="pfkB"
                     /locus_tag="VFQ23_10265"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET9907018.1"
                     /translation="MIYTLTLNPAIDRELTVAQVEFDTVLSAIKSQVDFGGKGFNVSR
                     LLKSMGMPSTALGFVGGRTGDRLRHGLQALDIQTDFVSISGETRTNISIVTEQHDHYI
                     KVNEKGPLVDESQQRELLDKIKTIAGAGDWWVLAGSLPPGVPDSFYAQITTILNERGA
                     TTILDTTGESLRLGCASGPFLVKPNAEEAHRLTGLPVENADQVAAAATEMRNMGAQNV
                     VISLGKKGALLQTADCTWLIHSPKIKEKNPIGAGDSMVGGLVWGLAQEYPLKESLGWG
                     AASGAATASMSGTEVGSRALIEELYERVEYETL"
     gene            complement(2161..3903)
                     /locus_tag="VFQ23_10270"
     CDS             complement(2161..3903)
                     /locus_tag="VFQ23_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9907019.1"
                     /translation="MGEKYKTYRSANYPLPAESWAWNLYGAGMENMGNHGKPEPFSIP
                     EPNDDQLLVRIDSVGVCFSDVKILKQGGSHPKLYNRNLSDDPTRLGHEVSLTIIKVGK
                     NLSGTYQAGQRLAVQPDIYQDGLSTAYGYTIPGGLVQYHLIGKEVLETDAGACLLPVE
                     DSMGYAESALLEPWGCVVAAYTQRRRLDPKPGGTMWIIGQRDDYTEFSFSKGLDAPAT
                     IILTDVPSSVKKLATATKAKLIEKNNLNADDYEGLSRELTDGKGFDDIVILNPASANT
                     VAKAATLIARRGTLNLVGTKALDGLVQVDFGRLHYDYIAFVGNSSPDIAASYGEQRNR
                     CDLRANGIAVFVGAGGPMGQMHVQRALELPNGPRLIIATEVNDERLATLNEMFAPLAE
                     KQGRTVIFFNPTNSEKSLHQFVMEISEGQGADDVVVSVPIAGLMAEADTVMKSDGMLV
                     FFAGVPNGTMGNVNLSDVYLSGAQYTGTSGLTIHDQALVMERRLEGTLSPGRSVAAIG
                     GLETAAEAIQSVMDSRYPGKVLIFPQIHNLPLTSLKELEERLPEVAARLGEDRMWTNS
                     AEEALIEKFWEEPA"
     gene            complement(3943..>5876)
                     /gene="ptsP"
                     /locus_tag="VFQ23_10275"
     CDS             complement(3943..>5876)
                     /gene="ptsP"
                     /locus_tag="VFQ23_10275"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET9907020.1"
                     /translation="HGEKKANAKSLISVLTLGASKGSQLRIQVNGEDEENALAEIESA
                     ILSGLGEGPEEGSTKPASTPVARPETAPVEVQQPASANSFRGVSAAPGIAIGPVFQFQ
                     RTEVGLDEMSDGLIMGWEKLQDALVQAREQLNHLNHQMIGKGLKAEAAIFEAHLELLA
                     DPELNEAVHTRVEKGQDALRAWKETIEDNAIIVAALNDPILSARADDLRDVGRRVLKI
                     MLGVEDKAASMPDGPTVVVARELSPSDTVSFNQDLVLGFCIVNGGPTSHTAILAKALG
                     LPAIVGVNESVLALESGTTVIVNGTEGTITFKPSDEELQAAQETRERWLERRRVALEQ
                     ANAPAITADGHEVEVVGNAGSLTDAQKALKMGAAGIGLLRTEFLFLERTTAPTEDEQF
                     DVYRSIAETMGKLPVIVRTLDVGGDKPLPYIEMQREENPFLGERGIRLCLNRPELFRQ
                     QLRAILRAAPHGNLRIMFPMVGDIDEYRQARAILDELHQSLNIPRIEAGIMIEVPSAA
                     LLADVFAREVDFFSIGTNDLTQYTLAMDRMHPALAGKQDGLHPAVLRLIAQTIDGAHK
                     HGKWAGICGELGSDPQAVPILIGLGIDELSVNTPSVPLVKAQIRSLKVAELKALARQA
                     LECSTAQEVRQLVRKHLSS"
BASE COUNT         1359 a         1670 c         1367 g         1480 t
ORIGIN      
        1 aagttgcctg aggcatgtat ttctccatat gatgtaggga cgggcagctg cccgtcccta
       61 cggatttctt gccagatctg ccaaatataa cgcgactgac ccggccacag ccgcgttcag
      121 tgattcgaca cgcccgcgca taggcagctt caacacgata tcacacttcg cgcgcgtaag
      181 ctgtcggatg ccttcccctt cgcttccgac gaccagggca acggggcccg ttaagtgacg
      241 ctgtgtcttc gccccgatcg tctcaccgtt ttgatccaat cccaccaccc agacgttttc
      301 ttcttttaaa gtatcaattg cctgtgcgag gtttgagcgt gaaatcagca tatgctcact
      361 cgcccctgag gatgcgttta cgaccgcagg cgtgacctcc actgtgtgtg ccagcgggat
      421 cactacccca tgcaggccga tggcctccgc ggtgcgaatc agtgtaccga aattttgtgg
      481 gtcattcaat gaatcaagaa gtaatacaaa agtcggctcg tttttttggc gggcattttc
      541 cagaatatca agcaaatcta catagggata tttgcccacc tcggccataa tcccttgatt
      601 gttgtcgtga attttatcca gccgtgcgcg cggcactttg acaacctgga tttttcgctg
      661 cgatgtcaaa gacatgattt cggcaagtct gcctttgatc tgcacattgt ctgccagttc
      721 aattttgaaa atatccctgc gtttggcgcg cagagtctca tagacagcat tgcgggaata
      781 gatgaattcc ttcattgaat tgattttact tgagaaccag cggcaatgta attagccccg
      841 atacatttcc accctaaaat agattatttc aagttgttct ccaaatgcgc aatgaaccag
      901 gtaatgacag tctgtgtcac ccctttacgc ccgccaaaca tcaacttgcg tccgacagca
      961 tccagcggat acgcatcaat gactaccttg cttccctcgc cattcgcttg aacaacgcac
     1021 gccaattggg tgcgatcgcc taacacctgt ccgagaatcg ttttatcaaa ctttacttcc
     1081 aggcgtaaat catcgggcga agaagaaaga agtttgccgc ccagtttttc cacggccttc
     1141 aacgcagcat catacacact gcgtccatcg tttgaataat ccttctcctg atgaacatcg
     1201 tacgacataa tacctccaag cctaaagagt ctcgtattcg actctttcat atagttcttc
     1261 aatcaaagca cgcgaaccaa cttctgtccc gctcatcgat gcggtggcag ctccgctggc
     1321 agctccccaa cccaacgact ctttcaacgg atattcctgt gccagccccc agaccaatcc
     1381 acctaccata gagtcacctg cgccaatggg atttttctcc ttaattttgg ggctgtgaat
     1441 cagccaggtg caatcggcag tttggaggag tgcacctttt ttcccgaggg agatcaccac
     1501 attctgagca cccatgttcc gcatttcagt cgcggctgca gccacttgat ctgcattttc
     1561 aactggcaag ccggtcaatc gatgtgcttc ctccgcgttc ggtttgacaa ggaagggccc
     1621 ggaggcgcag cccaatcgca ggctttcgcc agttgtatcc aaaatcgtag tggctccccg
     1681 ttcattgaga atcgttgtaa tttgagcgta aaaggagtct ggaactccag gcggcagact
     1741 ccctgccagc acccaccaat cgcctgctcc agcaatcgtt ttgattttgt ccaggagttc
     1801 gcgttgctgg ctttcatcca ccagtggtcc cttttcgttg accttgatgt aatggtcatg
     1861 ctgctccgtg acaatgctga tattggtgcg cgtctcacct gaaatcgaga caaagtctgt
     1921 ttgaatgtcg agcgcttgca atccgtgacg gagtctgtcg ccggttcttc caccgacgaa
     1981 acccagggca gtgctgggca tccccatgga tttgagcaaa cgtgaaacgt taaagccctt
     2041 gcccccaaaa tccacctgcg acttgatcgc cgataaaacc gtatcgaatt ccacttgagc
     2101 gaccgtgagc tcacgatcaa tagccgggtt gagcgtcaat gtatagatca tgatgggaac
     2161 ctatgccggt tcttcccaaa atttttcgat caaggcctct tcagcactgt tcgtccacat
     2221 tcggtcttcc cccagtctgg ctgcgacctc cggcagacgt tcttccaatt cctttagact
     2281 cgtcaatggc aggttgtgaa tctgcgggaa gatcagcacc tttcccggat atctgctgtc
     2341 catcaccgat tggatcgcct cggccgcggt ctccaagcct ccgatggctg ccacggaacg
     2401 accgggcgag agcgtccctt ccagacgcct ttccatgact aaggcttgat catgaatggt
     2461 caagccagag gttccggtgt attgcgcacc agataaatat acatcactca ggttgacgtt
     2521 tcccatcgtg ccgttcggca cgccggcgaa aaacaccaac atgccatccg atttcatgac
     2581 ggtatccgct tcagccataa gaccggcaat gggtacgctg acaaccacat catctgcccc
     2641 ctgaccttca ctgatttcca tcacgaactg gtgaagggat ttttcagagt tggtggggtt
     2701 aaagaaaatg acggtgcgcc cttgtttttc ggcaagcgga gcaaacatct cgttgagggt
     2761 cgccagtcgt tcatcattga cctctgtggc aatgatgagt cgcggtccgt ttggcaattc
     2821 gagtgcgcgt tgcacatgca tctgccccat cggtccgcca gcacccacaa agacggcaat
     2881 tccgtttgcg cgcaaatcac agcgattgcg ttgctcacca tacgacgctg ctatatccgg
     2941 gctggagttt cctacaaagg cgatgtaatc ataatgaagg cgaccgaagt caacttgcac
     3001 caatccatcc agcgcttttg tccctacgag attcaatgtg ccgcgccgtg caatcaatgt
     3061 tgcagccttg gcaacagtat ttgctgacgc tgggttgaga atcacaatat catcgaagcc
     3121 cttgccatcg gttagctccc tgctcaaacc ttcataatca tctgcattga gattattttt
     3181 ctcaatgagc ttagctttcg tagcagtcgc aagttttttt accgaagaag gtacatcagt
     3241 taagataatt gttgcaggtg catccagccc tttagaaaaa gaaaattcag tgtagtcatc
     3301 ccgttgtccg ataatccaca ttgtgccacc cggcttggga tccagcctgc ggcgttgagt
     3361 ataggcagcg accacgcacc cccagggttc caacaaagcc gactcggcat agcccatgct
     3421 gtcctccact ggaagaagac aagcgccggc atcggtttcc aatacttcct tgccaattag
     3481 atgatattga accaatccac ctggaatagt atagccatag gcagtgctca acccatcctg
     3541 ataaatatcc ggctgcaccg ccagccgttg accagcttga tacgtcccag acagattttt
     3601 gccaacctta atgattgtca gcgaaacttc atgccccagc cgcgtggggt catcagacaa
     3661 attgcggtta taaagtttgg gatgacttcc gccctgcttg aggattttta catccgaaaa
     3721 acagacacct acgctatcga ttctcaccag caattgatcg tcgttcggct cggggattga
     3781 aaacggctca ggttttccgt ggttccccat attttccatc ccggcgccgt agagattcca
     3841 tgcccagctt tccgctggca gaggatagtt tgccgaacga taggttttat atttttctcc
     3901 catgtatgtt tcctttcggg ggagggttag ggtgagggcg agttatgaac tcaaatgctt
     3961 cctgaccaac tggcgaactt cctgcgccgt cgaacattcc agggcctgac gcgccaaagc
     4021 ctttaactca gcgaccttca acgaacggat ttgtgccttc accagcggaa cagacggtgt
     4081 attgacactt agttcatcaa ttcccaaacc gatcaaaata ggaacggcct gagggtcaga
     4141 ccccagttcg ccacaaatac cagcccattt tccatgtttg tgcgcgccgt caatcgtctg
     4201 tgcgatgagc cgtaggacag cagggtgtag gccatcctgt ttgcctgcca aagccggatg
     4261 cattcgatcc atcgccagcg tgtattgcgt cagatcattc gtaccgatgg agaagaaatc
     4321 cacttctcga gcaaaaacgt ccgcgagcaa tgctgcagat gggacctcga tcatgatccc
     4381 tgcttcgata cgcggaatgt tcaaggactg atgcaactca tcgaggattg cgcgtgcctg
     4441 tcgatattca tcaatgtcac ccaccatggg gaacataatg cgaaggtttc catgcggagc
     4501 tgcgcgcagg atggctcgca actgctggcg aaacaactcc ggtcgattta agcaaagcct
     4561 gatgccgcgt tcccctaaaa acggattttc ttcacgctgc atttcaatat aaggcagggg
     4621 cttatcgcca ccgacatcca gcgtccgaac gattaccggc agtttgccca tggtctctgc
     4681 aatggaacga tagacatcga actgttcatc ttccgttgga gcagtggtgc gctcgaggaa
     4741 aagaaattcg gtccgtaaca agccgatgcc agccgcgccc atcttcaatg ccttttgtgc
     4801 atcagtcaat gagcccgcgt tgccgaccac ttccacctca tgaccgtctg cggtgatcgc
     4861 tggagcattt gcctgctcca acgcaacgcg gcggcgctcc agccaacgct ctctggtttc
     4921 ttgtgctgcc tggagttctt cgtccgaagg tttgaacgta atcgtacctt ccgttccatt
     4981 cacgatcaca gttgtgccac tttccaacgc cagcacagat tcattaaccc ctacgatcgc
     5041 tggcaacccc aacgcttttg cgagaatagc tgtatgagac gtgggtcccc cattgacaat
     5101 acaaaaacca agcacaaggt cctggttaaa tgacacggta tcagacggag aaagctcgcg
     5161 ggcgacaact accgtgggac catcaggcat ggaagcagct ttatcctcca caccaagcat
     5221 gattttcaga acacgcctgc ccacatcgcg cagatcatcg gcacgcgcag acaagatcgg
     5281 gtcgttcaac gccgcgacaa taattgcatt atcttcaatc gtttccttcc acgctcgtag
     5341 cgcatcctgt cctttttcga cgcgtgtatg gacagcttca tttagttcag ggtctgccaa
     5401 taattcaaga tgagcttcga agatagcggc ttcggctttt aatccctttc cgatcatttg
     5461 atggttcaaa tgattcagct gctcacgcgc ctgcaccagt gcatcctgta gtttttccca
     5521 acccatgatc agtccatccg acatttcatc gagtccaact tccgtacgtt ggaattgaaa
     5581 taccggacca atggcgattc cgggcgcagc gcttacccct cgaaaggaat tcgcagaggc
     5641 aggttgttgg acttcaacag gagcagtttc gggtctggca actggagtcg aagctggttt
     5701 cgttgaccct tcttccgggc cttcacccag tccagacaaa atcgccgact cgatttcggc
     5761 cagcgcgttt tcttcatctt ctccgttaac ttgaatccgc agttggctgc ccttggaggc
     5821 acccagcgtc agcacagaga tcaggctttt ggcatttgcc ttcttctccc catgcc
//
LOCUS       DASTJP010000343         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1182505, whole genome shotgun sequence.
ACCESSION   DASTJP010000343 DASTJP010000000
VERSION     DASTJP010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182505"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFQ23_10280"
     CDS             <1..967
                     /locus_tag="VFQ23_10280"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9907021.1"
                     /translation="NTFQRTLGRSGLQVSPMGLGCWAIGGVWSWLDGPGGWGDIDDNE
                     SVRAIQAALDLGINFFDTAANYGTGHSERILARALEGKRDKAVIATKFGFNVNEAEKR
                     VSFREDDHLLHVREECEASLRRLNTDVIDLYQLHVWNYPLDKVPEMVDLLESLVSDGK
                     IRHYGWSTDSVEGARLFANGKHCVAIQHDLNVILDAPEMLALCEELNLASINRSALAR
                     GALSGKYTKDTTFPQNDVRNDEWSKEHFFAPTLNQLESIREILTSNGRTLVQGALAWI
                     WARSEKTIPIPGFKTVAQVEENAKAIELGPLTTGQMKEIDSLLDR"
     gene            982..1923
                     /locus_tag="VFQ23_10285"
     CDS             982..1923
                     /locus_tag="VFQ23_10285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9907022.1"
                     /translation="MNLRPFGDTGMNVSEIGLGAWQLANPEWGINDKSEALRIIQRSL
                     EAGCNFFDTAPGYGGGRSEELLGEGLKSVRKDVIICTKFSHYDASGERDFDAANIRPV
                     LEGSFRRLQTDYVDMLLLHNPPRELMDGNVAPDIYEELEKLKAEGKIREFGISLDWRE
                     EVETAVKTTKSKALEVFFNALYQEPLPAFQMAQENGVGLIVKVPLDSGWLSGRYRGGH
                     RFDDVRNRWPAEVIARRTELIEQFAALVPAGTSLTHAALQFVLAQPQVSTVIPGAKTV
                     EQALENFAAAEKRLSAEVVQYMYDLWKREIESEPLPW"
     gene            complement(1977..>2369)
                     /locus_tag="VFQ23_10290"
     CDS             complement(1977..>2369)
                     /locus_tag="VFQ23_10290"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9907023.1"
                     /translation="TVARRWKGQLNEVAKAGANFEFLPEADHNTLAGTMNPQETLNAH
                     TMTLFLRAPSDHPRNRLRSDLTRKAFMLEGLNTDHVDGRGNTPLAHMWTLILFGDYMS
                     YYLAMGYGVDPTPIKALVEFKEAMAGAK"
BASE COUNT          608 a          542 c          665 g          554 t
ORIGIN      
        1 caacacattt caacggacgt tgggacgatc gggactgcaa gtcagcccaa tggggttagg
       61 ttgttgggcg atcggtggag tatggtcttg gctggatgga ccaggcggct ggggcgacat
      121 tgacgacaat gaatctgtac gcgccatcca ggctgccctt gatctgggca taaacttctt
      181 tgatacggcc gcgaattatg ggacaggtca cagcgaacgg attttggcac gtgcacttga
      241 aggcaaacgg gacaaagctg ttattgcgac aaaatttgga ttcaacgtca acgaagccga
      301 aaagcgcgtg agttttcgag aggatgacca tttgttgcat gttcgcgaag aatgcgaagc
      361 cagtcttcgc cgtttgaaca cggatgtcat tgatctgtat caattgcatg tttggaatta
      421 cccgcttgat aaggttccgg agatggtgga cctgttggaa tcgctcgtca gcgatgggaa
      481 aattcgtcac tacggatgga gcactgattc agtggaaggt gcgcgtctct ttgccaacgg
      541 gaagcattgt gtagccatcc agcatgatct gaacgtgatc ctcgacgcgc cggagatgtt
      601 ggctttatgt gaagagctga atctcgccag tatcaatcgc agtgcattag cacgcggcgc
      661 cttaagcgga aaatacacga aagatacaac gttccctcaa aacgatgtcc gcaatgacga
      721 gtggtcgaag gaacacttct ttgcaccgac attgaaccaa ctcgagtcaa ttcgtgaaat
      781 tctcaccagc aatggtcgaa ccctggtgca gggtgcactt gcatggatct gggcccgaag
      841 tgaaaaaacc atccccattc ccggcttcaa gacggttgcc caggtggaag aaaatgcaaa
      901 agccatcgag ttgggcccgt tgaccacagg tcaaatgaaa gaaatcgatt ccctattaga
      961 cagataatta aggaatattc catgaacctt cgtccgtttg gtgatactgg catgaacgtg
     1021 agtgagatcg ggctgggcgc ctggcaactg gcgaaccccg agtggggaat caatgacaaa
     1081 agcgaggcac tccgcattat ccaaaggtcg ttggaggcag gctgcaactt tttcgacacg
     1141 gcaccaggtt acggcggagg aagaagtgaa gaactgcttg gtgagggctt gaagtctgta
     1201 cggaaagacg tcatcatctg caccaagttc agtcactacg acgcgtccgg tgaaagagat
     1261 tttgatgcag cgaacatccg ccctgtgctt gagggaagct tccgtcgcct gcaaaccgat
     1321 tatgtagaca tgctgcttct tcataacccc ccgcgtgagc tgatggatgg taatgttgcc
     1381 ccggatattt acgaagagct ggaaaagctc aaagcagaag gcaagatccg tgagtttggc
     1441 atttcactcg attggcgcga ggaagtggaa actgcggtta agacaacgaa gagtaaggca
     1501 cttgaagttt tcttcaacgc tttatatcag gaacccttgc ctgcatttca gatggcacag
     1561 gagaatggtg tggggctgat tgtcaaagtg ccgttggatt caggctggct ttctggtcgt
     1621 tatcgaggag gtcatcggtt tgatgatgtg agaaatcgct ggccagcaga ggtgattgcg
     1681 cgacgtactg agttaattga gcaatttgcc gcactcgtac cagcgggaac gtcgctgact
     1741 catgccgcgc ttcaatttgt actggcacag cctcaggttt cgacggtgat ccccggcgcg
     1801 aaaacagtgg aacaggcact ggagaacttc gccgcggcag agaagcggct tagtgccgag
     1861 gtggttcaat acatgtacga tttgtggaaa cgagaaattg aaagtgaacc cttgccatgg
     1921 taagaaagag aattcccttt aaaagcaaaa aggcgacccg ttgaggtcgc cttgatttat
     1981 tttgctccgg ccatcgcttc tttaaactcc accagcgcct taattggtgt gggatcaaca
     2041 ccgtagccca tcgcaagata atacgacatg tagtcaccaa ataaaattaa tgtccacata
     2101 tgcgcgagag gagtattgcc acgtccgtcg acatggtctg tgttcaaacc ttcaagcata
     2161 aatgctttgc gcgtcaggtc tgagcgcagg cgattacgcg gatgatccga tggtgcccgc
     2221 aggaaaagag tcattgtgtg ggcgttgagc gtctcttgtg gattcatcgt cccggcaagc
     2281 gtattgtgat cggcttcagg aaggaattca aaatttgcgc cggctttggc aacttcattc
     2341 aactgaccct tccagcgacg ggcaaccgt
//
LOCUS       DASTJP010000344         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_328371, whole genome shotgun sequence.
ACCESSION   DASTJP010000344 DASTJP010000000
VERSION     DASTJP010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328371"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFQ23_10295"
     CDS             complement(<1..437)
                     /locus_tag="VFQ23_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HET9907024.1"
                     /translation="MSILSKKRIILGVTGSIAAYKSADLASKLTQAGAQVDVILTGAA
                     EKFITPLTFQSVTGRRAYTDSDLWGNEAHVLHVGLGHSADLILIAPCTANTIAKLAHG
                     QSDTLLTVTALASRCPLMIAPAMDGGMFDHPATQENIEILEKRG"
     gene            complement(471..1568)
                     /locus_tag="VFQ23_10300"
     CDS             complement(471..1568)
                     /locus_tag="VFQ23_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9907025.1"
                     /translation="MNISNSIIQRARESGNPVIEGNQAILIWEGKHTPYLISDLNEWD
                     EKATPFKRLSSGRRPASAEPIWYYSVTLPRDAYIEYAVYIPKTKTRLLDPLNRRTVSN
                     GFGARNNYFYMPESVPSPFVVRRADVPAGTVKRHRVDTSWMQEDSKREVYLYKPPVKR
                     AVPLLVVYDGYDYLYRTRLATMVDNMIAEKRIRPIAMAFLQNGKSRRNVEYFCSDATI
                     TWLDREVLLLAEKHLNLLNIKKYPGAYGVLGASAGGLMSMYTGLRMPEIFGKVLCQSG
                     VFGLDGRDFAVVDLIRHRHAQNIKIWMDVGKFEWLLGDNRRMQPILKENGYQFIYREY
                     SGSHNYTSWRDDLWRGLEAMFPLKSDSHLER"
     gene            complement(1565..2014)
                     /locus_tag="VFQ23_10305"
     CDS             complement(1565..2014)
                     /locus_tag="VFQ23_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET9907026.1"
                     /translation="MTIQLRLTSDAFANGQSIPAKYTCKGKNISPALAWTEPPAGTQS
                     LALIMDDPDAPGGTWVHWLLFNIPVNTRTFPEDLRLSGVSVGKNSWGDMQYGGPCPPG
                     GTHRYFFKLYALGSNISLSPGFTKDQLLKAMDGHILAQGELVGTFSK"
     gene            complement(2030..>3105)
                     /locus_tag="VFQ23_10310"
     CDS             complement(2030..>3105)
                     /locus_tag="VFQ23_10310"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HET9907027.1"
                     /translation="QTYLYFMKTFLIYGSYGYTGQLIVDLAVKDGLHPILAGRDEKKL
                     RVQAKKFNLEYRAFSLDETSKLDSSLKEVEAVLHCAGPFVHTFRQMAEACLRNKKHYV
                     DISGELAGFEALAEMDDQAKRAGIMLLPGGGFDVVPSDCLAAHVKQRLPSATHFRLFL
                     KGVGAGASRGTAKSSIENMHRQGRIRRDGKVVQVPPAWRVLKQDFGGGPVNVVSVGWG
                     DVSTAYYSTGIPNIETYFAFPRVMIDFLYGLRVLGPLFYHRVAKTFLKALVDTVLPPG
                     PSEEKRKKRFAIMIGEVIDARGNRAVSKLIIPEGYTCTALTTVEIMKRILNGDYKVGF
                     QTPSLVYGSDFILQFAGVKREDL"
BASE COUNT          768 a          797 c          748 g          792 t
ORIGIN      
        1 ccacgctttt ctaatatctc aatattctct tgtgttgccg ggtgatcaaa catcccgcca
       61 tccatggcgg gcgcgatcat cagcggacat cgggatgcta aagccgtgac agtcaataac
      121 gtatccgatt gaccatgcgc caatttggcg attgtattgg cagtgcaggg agcaatcagg
      181 atcaagtctg ctgaatgccc gagacccaca tgcaagacgt gtgcttcatt tccccataga
      241 tcagagtcgg tataggcgcg ccgtcccgtg accgattgaa atgtaagtgg tgtgataaat
      301 ttttctgccg cgccggtcag aatgacatcc acctgcgcgc cagcctgcgt tagtttcgat
      361 gcaaggtctg cggatttata agccgcgatt gaacctgtaa cgcccagaat aattcttttc
      421 ttagaaagga tggacatatt ccttattata cttctgctca aaagtgacaa tcatcgttca
      481 aggtgactgt cactcttgag aggaaacatc gcctccagcc cgcgccagag gtcatcgcgc
      541 caggaagtgt aattatgtga gccggagtat tcgcgataga taaattgata accattttct
      601 ttcaagattg gctgcatgcg acggttatct cctaacagcc attcgaattt tcccacatcc
      661 atccagatct taatattttg tgcatgccgg tgacggatca gatccacgac tgcgaagtcg
      721 cgtccatcca gcccaaacac gccagattga cataacacct ttccgaaaat ttccggcatt
      781 cgcaaacctg tatacatgga catcaatccg ccagccgacg cgcccaggac gccatatgct
      841 ccgggatatt ttttaatatt gagaaggttc aagtgttttt cagccagcag caggacttcg
      901 cgatccagcc atgtaatggt cgcatcagaa cagaagtatt cgacgttgcg ccggcttttc
      961 ccattctgca ggaaagccat cgcgatgggg cgtatccgct tttccgcgat catgttatcc
     1021 accatggttg caagcctggt tcgatacaga taatcatatc cgtcatagac caccagcaga
     1081 ggtacagctc gcttgacagg tggtttgtac agatagacct cgcgtttact gtcctcctgc
     1141 atccaggatg tatctacgcg gtgacgtttc accgtgccag caggcacatc cgcgcggcgc
     1201 actacaaatg gagacggcac ggactcaggc atgtagaagt agttgttgcg agcaccaaaa
     1261 ccattgctga cagttcgcct gtttaaaggg tccagcaggc gcgtttttgt tttgggaatg
     1321 tagaccgcat attcaatgta ggcatcgcgc ggaagcgtga cagagtagta ccaaattggt
     1381 tccgcggaag caggccttcg tccggatgag aggcgcttga agggcgtggc tttttcatcc
     1441 cattcattca ggtcactgat caaataagga gtatgctttc cttcccaaat gaggatggct
     1501 tgattcccct cgataaccgg atttcctgac tctcttgccc gttgaatgat tgaatttgaa
     1561 atattcattt actgaacgta cctaccagtt cgccctgtgc gagaatatga ccatccattg
     1621 ccttgagcaa ttgatctttt gtaaagccgg gagatagact gatgttacta cccaaagcgt
     1681 agagcttgaa aaaatatcga tgagtaccgc caggtgggca gggacctccg tattgcatat
     1741 cgccccatga gtttttaccc acgctgaccc cgctgagacg caggtcctct ggaaaggtgc
     1801 gcgtgtttac tggaatgttg aataataacc aatgcaccca cgtccctccg ggcgcatcag
     1861 ggtcatccat gatcaaggca agagactgtg tgcccgcagg tggttctgtc cacgctaaag
     1921 caggagagat gttctttcct ttacaggtat atttcgccgg gatcgattgc ccatttgcaa
     1981 atgcgtcact ggtgagcctt agctgaatcg tcatcagtgc atccattgtt tagagatcct
     2041 ctcgtttcac accggcgaat tgcaatatga aatctgagcc atagacaaga gacggggttt
     2101 ggaagccaac tttgtaatca ccgttcagaa tccgtttcat gatttcaacc gtggttaagg
     2161 ccgtgcaggt gtatccttcg gggattataa gttttgatac agcgcggttg cctctggcat
     2221 cgatcacttc gccgatcatg atggcaaatc gtttcttcct cttttcctcg ctgggtccgg
     2281 gcggcagaac tgtgtctacc agggccttga gaaaggtttt cgcaacgcga tgatagaaca
     2341 gcggtcccag cactcgcagt ccatagagga agtcgatcat cacccgggga aatgcaaaat
     2401 atgtttcgat atttgggatg cctgtgctgt agtatgccgt gctcacatct ccccaaccaa
     2461 ccgatacaac gttgactgga ccaccgccaa aatcctgctt gaggacgcgc cacgctggag
     2521 gaacctggac tactttgccg tcgcgacgaa tccttccctg ccgatgcata ttctcgatag
     2581 aagactttgc tgtgcctctc gaagctcccg cgccaacacc tttcagaaac aggcgaaaat
     2641 gagttgcgga aggcagccgt tgcttgacgt gtgcagcaag acaatcggag ggtacaacat
     2701 cgaatccacc tccgggcagc agcatgatgc cagcccgttt ggcttgatca tccatctcgg
     2761 ccaatgcctc aaaaccggca agttccccgc tgatatccac ataatgcttt ttatttcgca
     2821 gacaagcctc ggccatctga cgaaacgtat gcacaaatgg acctgcacaa tgtaggaccg
     2881 cctctacttc cttcagagaa gaatccagtt tggaagtttc atccagtgaa aaggcgcgat
     2941 attcaagatt aaatttcttt gcctgaacac gcaatttctt ttcatcgcgt cctgcgagaa
     3001 tcgggtgcag gccgtctttt acagcaagat caacaatcag ttggcctgtg tatccgtacg
     3061 aaccatagat gaggaaggtt ttcataaaat atagatacgt ttgta
//
LOCUS       DASTJP010000345        18052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_584682, whole genome shotgun sequence.
ACCESSION   DASTJP010000345 DASTJP010000000
VERSION     DASTJP010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18052
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584682"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFQ23_10315"
     CDS             complement(<1..346)
                     /locus_tag="VFQ23_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907028.1"
                     /translation="MNFPAVPVPSYKHPTVWLTIARVFQILITCVAIGLFIMSIPIYY
                     EHRSSVCTNEPCPPDQISPAGERALERMGLSAESYAALTLVLEMLVAVTFIACAVIIF
                     LRKPNNLFAIFVT"
     gene            737..2893
                     /locus_tag="VFQ23_10320"
     CDS             737..2893
                     /locus_tag="VFQ23_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9907029.1"
                     /translation="MSTKAKILFVVVLLASVSAGIIWRLQNPPGVIMINPMRPPQNIL
                     GEAELEKLRALQNTTDIFLIGSISPDDSTVIVATGTEEGSENEGSKQVSWMNIQTGQL
                     EPVDSKFLELFPQGEIAWPDNNTAVYLSSNLNGDPVLVTLQRSTDEMQTKPLEISGRP
                     LSLAPNGSRLLIESGSENKIDLSIMDVASGATEKLLSYHGTTPLSIAWTPDGSKLAII
                     HLVLPGDKVDEKWGLEISHQDATGNVPLDQNPFYAGNVVDIFDFQQGVSHLEALNAGE
                     DGDGYYFNKVFWSPDGRRLLAKMLRPSQPKGRSNPVVLLGQFADRANYRVYDADLHWI
                     ETLDQPELEAPNSALSPVAGDAMFVSADEVVIVAAYGLNMHLFYSNLATGEFRRLPTE
                     PGTFDMAPGGYQVYFTHQSRQVLYTQSSFQHPSEIHRLDLDGGAPQLLLKYNDSAVAA
                     NQIRVDEVRFELSEDIVRVGYLLQPAGGTFPPQNIPIVIYHQGGPGGAMTNHWGKSPD
                     DPWNLLPNFGIGLLFMPFSGREGFGPEFYRALADADNFGQIDVAESALAARYLIEHGY
                     SARGQIGIAGCSYGGYVVNQSLIQYPELYAAGNAQCSIVDIAAWWEDNPLMVDFYQGA
                     RPAQKPDEYRWDSPMHNAAQINTPLLLFHGVDDNLLPFRLVKQFRTEVESAGVPVRFL
                     AFKSEGHGLSFPSSQLIAAQEQIVWFREYLGIQPSQ"
     gene            2960..3691
                     /locus_tag="VFQ23_10325"
     CDS             2960..3691
                     /locus_tag="VFQ23_10325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9907030.1"
                     /translation="MNNKFEQHPIPVSIFLHLLPGILTGIAFFAVAPVAQRMNFPPFV
                     AHAFADLVILLPVIYGILFYQGFRKNGRLSLEGVVLYRERIAWWKYFVYVPIVVVASL
                     IFLLLSPVSDAIREGLFSWWPSMYELSLDLSSYPRSTAIISVVINFLMICLAAPITEE
                     IYFRGYLLPRLSRFGFWAVPIHTTLFGLFHTWTPWMAVTRAIGIIPFAYIVQRTKNIY
                     IGIIAHVLFNLIDLIPAMLLVLQRT"
     gene            3745..4572
                     /locus_tag="VFQ23_10330"
     CDS             3745..4572
                     /locus_tag="VFQ23_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9907031.1"
                     /translation="MKKTISTYPLVSFFVMSILFTWIAVIPLLQNPTLPVEPFMILGA
                     LAGPTLSAVIVIATTEGKAGLAAFFKRYIQWKAGIVWWLIVLFGILLSLNIVASMILG
                     ASIWTEFFRNLGLILPTYLITLIVGVILGPLWEEPGWRGFALPRLQEQYGPLIGSIIL
                     GVIWAVWHAPGYLGGWMLSTVPALLVYSIGFSILATWVYNNTRSSILLMILLHSSSNA
                     AISVGARVLPTELSPALHNFVYSGWIPAIMGGVIAILILIFTRGTLGYSKAVSRSSL"
     gene            4588..5388
                     /locus_tag="VFQ23_10335"
     CDS             4588..5388
                     /locus_tag="VFQ23_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9907032.1"
                     /translation="MALLQASIVNSKTFDSAGSASAPAIVLVHGSVVTRKMWLPQLRG
                     LSDTFHVIAPDLPGHGALAEMPFTFATAAQNLADVIRHEANGRALVAGLSLGGYIALE
                     LVRRSPELVSGLVLSGCSLNFDGFLGSYLKFASQLMKRGWIKLGKEKSEARVRRMFPP
                     ALADVAEEQIRAGVYPDALAPSYIEMAHRDFSAALAEYPGPSLILNGERDSSSRKGEV
                     KFLTAAQQGRVQTILDAGHACNLDQPERYNQAVREFSQSIGWTFAQTG"
     gene            complement(5399..9286)
                     /locus_tag="VFQ23_10340"
     CDS             complement(5399..9286)
                     /locus_tag="VFQ23_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9907033.1"
                     /translation="MQRSAAGELKPFIMEALYAYIPTDRRFALARGYDLPDHTTGAAL
                     FADISGFTPLTEALVQVLGPQRGAEELTRWLNEIYDALLEDIERYQGSVIGFSGDAIT
                     CWFDDQNELLLHSQPKTSSIRRAAASAMAIQQTMRRFAQVDIGGAGNVSLAIKVVVTA
                     GPARRFLIGDPTIQLVDALAGKTLYRLATAEHHANRGEVLLDQAAVTALSDQIDVLEW
                     REDSESGERFAVVDHLKTDVEPDPWPALDTNALNADLLRPWLLPPVYDRLMSGMGEFL
                     TELRPTVALFLRFSGIDYDDDPEAQSKLNAYLQAVQQILARYQSYMLQLIIGDKGSNF
                     YVSFGAPVAHEDDAIRAVRAALDLRDLQMDFITDIQIGVSQGLSRTGACGGIYRRTYG
                     AIGDAVNMAARLMQNAPVGQALVNHNVRKATGEEFIWEGLPPLRVKGKSEPVTVFRLI
                     SNQEHHGIRLHEPKYALPMVGRRTELALIEQKLDIAFRGHGQIVGITAEAGMGKSRLM
                     AEVIRHARRRNVLGYGGECQSYGTSTSYLVWQNIWRGFFDLDPNDSVAEQIESLERQL
                     SEIDPRLLPRLPLLGAVLNLPIPDNELTSSFDAKLRKESLESLLVDCLRKRARSMPLF
                     LVLEDCHWLDTLSHDLLEVIGRQIVDLPIFIAIAYRPPQLSVLLAPRVSKLFHFTAIP
                     LADLPAEEIEQLVLFKLRRIYGKQAKLPQALIEKISTRAGGNPFYIEELLNYLHDRNI
                     DPEQPEALEQIDLPDSLHSLILSRIDQLSENQKTLLKVASVIGRLFRVAILWGVYTQF
                     GDQERVRSDLDALSALDVTPLDTPEPELAYLFKHILTQEVAYETLSFATRAMLHDQIG
                     KFIEKIYEDTLDQYTDLLAYHFERSDNQPKKREYLLKAAEAAQADYANTAAITYYQRV
                     LHLLPEAERVGGLLKLGQVFELIGEWENAEEIIREAERLATQINDAQSQTQAGRAQGW
                     LMRKRGDYNAAEERLMRARDGYERLGDSAGVSNVLADIGEIYRLQGKFAEARAYYDES
                     LNLSAGIADTRLRQKARAHALKGAGTVATWQGDYATARELNSESLALRRELGDTPGVA
                     TLLNNLGIIARFQRDLTSAREMNEESLALFRAMGDQWAVGQLLNNQACVASDQKEYAE
                     ARVLLEESLMIRRKLGDKAGLALSLNTLADVVLDEGEFGDARPLLDESLTINRELGDQ
                     TAIAYLIEDYAGLAAAEAKAQRALQLAGFAAALRESIGAPLPPAEQARVDKMIEPARQ
                     VLAESTVTAEWDAGRSLALEQAIELALSNGDIS"
     gene            complement(9513..16607)
                     /locus_tag="VFQ23_10345"
     CDS             complement(9513..16607)
                     /locus_tag="VFQ23_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HET9907034.1"
                     /translation="MLKQLARITYIFLILTILLAQFGVRPALAEADFTVNSTADMVDA
                     FPGDGLCETDITGDCTLRAAVQEANARDGANTISIPAGVYTLTIEGADENAAATGDLD
                     IFDNVTIVGAGMGATVIDANGIDRAFQVQSWGVELSELTIKNGTASPGGAILVNGHVN
                     INRVTFDDNRATSTDSNGTGGAIHVTVEASADISESRFTNNFAHFGGGAVSNTYASSF
                     TITDSTFDLNSSVFGGGALYPNGGSVTVNNSTFFGNSADTGGAIHSNSDFVEVTNSTF
                     VDNEAVNHGAIDSRVGTIIVNNSTFSGNRASGSGDTLGAQRGQGGELRVSNSILSGDG
                     LNNCETVTDLGNNLSWPIENNCPGTQADPLLDTLADNGGSTETMALLTDSPAIDAGDI
                     ESCASTDQRGVTRPQGETCDIGAFESVFTNILVVTNTNDSGEGSLRQAIVDANALPNS
                     VNGADEIHFNIPSEGINIISPATPLPAITEAVVIDGYTQFGASQNTLSVGNNAVIRVE
                     LLGDSCDGLCGQGLLLSSGGSTITGLSIHGNFNNGIEVNGSGNTITGNFFGLGGNGTA
                     DGVVASGIYLNNTSDNIIGGSTPAARNVISANRDGIFIAAGAGPATNNVITGNYIGTN
                     PAGTSAMGNSQRGIFVGSFGQTATENSISGNVISGNGRFGILLRDSNVTGNFVTGNLI
                     GLNANGTDPLPNGNNDSGDDGLAGTSATAGIYVAGPNNTIGGIGRVSANTIAFNTGAG
                     VQVGAATGTDIRGNNIFSNSGLGIDLAGDGVTFNHVGFVAGPNNFQNFPVMSVATSDG
                     ESLRVGGYLESEANQNYSIDVFANESCDPTFFGESESFLGSFVVSTDASGVAVFDQNL
                     SAGADEPFGISATVTGNNGTSEISYCRPVATPNLNWAQAQSVSDDSKTQQFITDVFQE
                     KWFKFPVQPGATVNIKLTSLPGSAVSLHRDPHPIYNALIDPQNAALLSAEASDAAFLP
                     SGSLPSGSLPSGSLPSGSLPSGSLPSGSLPTGYLPSGSLPSGSLPSGSLPSGSLPSGS
                     LPSGSLPSGSLPSGSLPSGSLPSGSLPSGSLPSGSLPSGSLPSGSLPSGSLPSGSLDA
                     YGSAAINSLMGISMDPYATVQTIERNTYDLQEDLYVRVVGPYDLETPFTLDVFVEGGV
                     CGDVDVVPSGLQVIAGTPSAGSHETLLLTDSARLHGTPGEIAAALDDIDVLAARSDVN
                     GVVIDLSDAQYERVAFANAEADANLACAAAKNTVAKEIKQVIDEYRDANPTLKYIVLA
                     GGADVIPFFQVQDVSGLAFEKEYVVPVAPSTASEAGLKTNLVQGQDAYGSQIDFTQAG
                     YTLALPDLAVGRLVDTASEISTAIDAYIETDGIIEPTSSLVTGYDFVGDAAVAIQAEM
                     DAGTDSTADTLIQAPGLPPTDASAWTANQLRSKLQGGNFDIAVLTGHFSAGNLLAADY
                     ATELSATEIAAMTDMTDVLFLALGCHGGYSIPNSDLLNGISPNPDWAKAFLANGAAGY
                     ISATGYAYGDTELVEYGERLFLLMAQQLRTGNDPVAVGQAVVKAKQQYLAETAQLTGI
                     DHKTLVEMTLYGLPMMKVNMPGARLNPPADNSIIGSTSPVGATPGANFGLRRSPVVLN
                     PVVNTHNKVLQNLSGGGTVTTTYLSGADGVVVNPFEPIYPKEIFNVTSSGNVLRGVAL
                     RGGTYTDLAGIVPLTSSPTTETSTAHLSFNTNVFYPTQTWASNFSDAISGGQTRLILF
                     PAQFQSSAPNAIDGILRKFNQLDLELYYLSADWTNAGSPETKAAGVSASPVILGASGE
                     ENGNTIAFSVNAATDGSAGVQAVWVLYTGKPGSSYHGTWVPLDLTQNTNDPTLWEGTL
                     TLQSGENAEDILFMVQAVGGAGLTTLATNLGAYYGVGDENATQLPPPAGTTLTLQSPP
                     ASGTYLKESSFDLLLQSGGQPLANQLVSIDIGGQQASALTDSSGEATLTLNLVVRPGN
                     YNAQARFGGNAQYSGSNDVSDFTVNKDSTTLTVTPASASIFTNQATPFVAVVRDSDGR
                     ALGGKSIFFIVHNSTDSFATSVIADFAGNATLGSVPLPPGTYTVDAYFNGTIPVSPSL
                     TLSDDYYESSSDLGSSLTIVGDTTLPTITATATKADTTAYTADTWTNQTVTVHFTCSD
                     TESGIASCPADQIFSVDGTFTATGTATDNLGNSASTSFGPIKIDKTGPTLAPSVSPNP
                     VLLNGTAIASANATDTGSGIASQSCGAVVTNTVGSKTVTCSATDNAGNTAVANAEYRV
                     IFNFTGFFSPVDNPPVINQMNAGRSVPIKFRLDGNQGLAIFEAGYPLSQQVQCTNLAL
                     IDEVEQTSTAGSSSLTYDAATNTYNYVWKTEKSWAGTCRLFTVEFIDGQSYTLLFKFK
                     "
     gene            17000..17986
                     /locus_tag="VFQ23_10350"
     CDS             17000..17986
                     /locus_tag="VFQ23_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9907035.1"
                     /translation="MPNARWTAEKANEWYEGLPWLVGCNFIPSTAINQLEMWQPETFD
                     PETISRELKWAAGIGFNVVRVYLHNLLWESDQVGFEERIKRFLDIAASHQIRTVFVLF
                     DDCWNTNPQLGKQPEPKPGIHNSGWVQSPGSKLVTDESSWDTLADYVRGVIRTFAQDE
                     RILMWDLYNEPGNNGLDEKSLPLLQSAVTWAREANPSQPITIGLWYENESLNDFQLYA
                     SDVITFHNYLPEDDLLKQITTLKEHGRPLICTEYMARTRGSLFEKSLPIFKRENVGCI
                     NWGLVNGKTQTIYPWGSPGGEAEPELWFHDIFHRDGKPYRQEEVDFIKEITG"
BASE COUNT         4309 a         4647 c         4712 g         4384 t
ORIGIN      
        1 cggttacgaa gatggcgaat agattattgg gcttacggag aaagatgatc acggcgcagg
       61 cgatgaaggt cacagctaca agcatttcaa ggacaagggt cagcgccgca taggactccg
      121 ccgacaggcc catccgttcc agggcccttt cgcctgcagg gcttatttgg tctggcggac
      181 acggctcatt cgtgcacacg ctgctgcgat gttcgtagta aatcgggatg ctcatgatga
      241 acaaaccgat ggctacacag gtgataagga tctgaaagac acgggcgatc gttagccaga
      301 cagttggatg cttgtatgaa gggacaggaa cagctggaaa attcattacg gttcataagc
      361 ataccgcaat tttttcccga tgtcatcgga aaatttccca gatagccggg aataacttcc
      421 caccaattga tgcctcatcc cccatccccc caggaattgg gaaatgtaag atggggtcat
      481 gcgtttttag tgccacatat cggcccgctc tggtcatcaa ccgtccatgc caatatttat
      541 agtagagaaa gcaatcattc tcccaatggt ggccaggttc aggaaatgta ctattttttg
      601 tctgatggtt tgtacgaatt cttcccacca aattaacggt gcaaagaata aattatggac
      661 aactcatctc aatcaaatgc aatgtcaaag tctgaagttt ggataaaagt ctgctaatga
      721 ctttggagga ggcaaaatgt ctacaaaggc aaaaattctt ttcgtggtgg ttctcctggc
      781 aagcgtatca gcgggaataa tctggcgtct gcaaaaccca ccgggcgtca taatgatcaa
      841 cccgatgcgg cctcctcaga atattctagg cgaggcggaa ttggagaagt tgcgcgccct
      901 acagaacacg acggatatct tcctgatagg aagtattagc ccggacgatt cgaccgtcat
      961 tgtggcaacc ggcactgaag aaggatcaga aaatgaaggt tcgaaacagg tttcctggat
     1021 gaatattcag accgggcagt tggaacccgt cgattcaaaa tttctggagc tatttccaca
     1081 gggcgaaatt gcctggccag ataacaacac ggctgtctat ctttccagca acttaaatgg
     1141 agacccggtt ctcgttaccc tgcagcgatc aacggatgaa atgcagacca aacccctgga
     1201 aatatcgggc cgacctctca gccttgcgcc gaacggctca cgcctcttga tcgagtctgg
     1261 gtcagagaac aagatagacc tgagcatcat ggatgtggca agtggagcga ctgaaaaact
     1321 gttgtcgtat catgggacca cgcctctttc cattgcctgg acgccggatg gaagcaagct
     1381 tgccatcatt caccttgtcc tgccgggcga caaagttgat gagaagtggg gtctcgaaat
     1441 tagccatcag gatgccactg gtaatgttcc tttggatcaa aacccgttct acgctggaaa
     1501 tgtagttgac attttcgatt ttcaacaagg tgtatctcat ctcgaggcac tcaatgccgg
     1561 cgaggatgga gatggatact atttcaataa agtcttttgg agtccggatg gtcgacgttt
     1621 gttggcaaaa atgctccggc cctcacaacc gaaaggacgg tcgaatcccg tcgtgctgct
     1681 cggtcagttt gctgatcgag ccaactatcg agtctatgat gctgacctgc actggatcga
     1741 gaccctggac cagccagaac tcgaagctcc caattcagct ctgtcacccg ttgctggcga
     1801 tgccatgttt gtgtctgcgg atgaggtggt tatcgttgcg gcgtatgggc tcaacatgca
     1861 cctgttctat tccaacctag cgactggcga gttccgcagg ttgccaactg agcctggtac
     1921 ctttgatatg gcacccggag gataccaggt gtatttcacg catcagagcc gacaggtgct
     1981 ttatacgcaa tcatcgtttc agcatccatc ggagatccat cgccttgacc tggatggcgg
     2041 cgcgccccag ttgcttttga aatacaacga ctcagccgtc gcggccaatc agatccgcgt
     2101 ggatgaagta cgcttcgagt tgagtgagga tattgtccgt gttggttatt tgctacagcc
     2161 tgccggaggg acattcccac cgcagaatat tcccatcgtg atctatcacc agggaggtcc
     2221 gggtggagcg atgacgaatc actgggggaa gagtcccgat gacccctgga atctgctacc
     2281 taatttcggt atcggtctgc tctttatgcc attctcgggc cgagaagggt ttggaccgga
     2341 attctatcgc gcactggcag atgcagataa tttcgggcaa atcgatgtgg cagagtccgc
     2401 gcttgcagcc cgttatttga tcgaacatgg ctattctgcc agagggcaga ttgggatcgc
     2461 aggctgctca tacggcggat atgtagtcaa tcaaagcctt attcaatatc ccgagctcta
     2521 cgctgctggc aacgcacaat gctccattgt cgatatagcg gcgtggtggg aagacaaccc
     2581 attgatggtt gatttttatc agggtgcccg gccagcccag aagcccgatg agtaccgttg
     2641 ggattcgccc atgcataacg cggctcaaat caatacaccg cttctgctgt ttcatggtgt
     2701 ggatgacaac cttttgccat tcagactggt gaagcaattt cgaacggaag tcgagtcagc
     2761 gggagtccct gtgagattcc tggctttcaa aagcgagggg cacgggctgt cgttcccctc
     2821 cagtcaactg atcgctgcac aggaacaaat cgtttggttt cgggaatatc ttggaatcca
     2881 accgtctcaa tgagacttgt tccgtagtgg aatcccacta ttctaattct gattatctga
     2941 aggaataaga tgaataacaa tgaacaataa atttgaacaa cacccaatcc ccgtgtcgat
     3001 ttttctgcat ttgctcccgg gcatcctgac ggggatcgcc ttctttgcag ttgcgccagt
     3061 cgctcaaagg atgaactttc ctccttttgt agcacatgcc tttgcagatc tggtgatcct
     3121 tctgcctgtg atttacggca tcctgttcta tcaggggttc aggaagaacg gacgcctgtc
     3181 tcttgaagga gtagttctct atcgggaaag gattgcctgg tggaaatact ttgtctacgt
     3241 gcccatcgtg gtggtcgcca gtctcatctt tcttttgctt tccccggtga gcgatgccat
     3301 ccgtgaggga ttattttcct ggtggccctc gatgtatgaa ctgtcgctgg atctgagcag
     3361 ttatccgcgc tccactgcga tcatttccgt ggtcattaat tttctgatga tctgtcttgc
     3421 tgcgccgatc acagaagaaa tttattttcg tggctacctg ctgccgaggc tttcgcggtt
     3481 tgggttttgg gcagttccca ttcacaccac actgtttggg ttattccaca cttggacgcc
     3541 gtggatggct gtcacgcgtg ccatcggcat cattccgttt gcgtacatcg tacagcgaac
     3601 aaagaacatc tatattggca tcatcgccca tgtccttttc aatctcatag atttgatccc
     3661 agccatgcta ttggttctac agcgaaccta aaagactgcc tgttggttat ctttatagac
     3721 ctagaactgg aaagaggaaa caaaatgaaa aagacaattt caacttatcc cctggtctcg
     3781 ttttttgtaa tgtccatcct atttacctgg atcgctgtca tacccctgct ccaaaatccc
     3841 acgcttcctg tggagccttt tatgatcctc ggcgcgctgg caggacccac actgtctgcg
     3901 gtgatcgtga tcgcaaccac agagggcaaa gccgggttag ctgctttctt taaacgctac
     3961 attcaatgga aggccggcat cgtctggtgg ctgatcgttc tgtttggaat tctcctctca
     4021 ttgaacatcg ttgcatccat gattctcggt gcatcgatct ggaccgaatt cttcagaaac
     4081 cttgggttga tcctccccac ctatttgatc accctgatcg tcggtgtgat cctgggtcca
     4141 ctatgggaag aaccaggttg gcgcggtttt gcgctgccgc gtctccagga acagtatgga
     4201 cctttgatcg gttcgatcat tttgggagta atctgggctg tgtggcatgc accaggttac
     4261 ctgggtggtt ggatgctctc gaccgttccg gcgctcctgg tttacagcat tggattttcg
     4321 atccttgcga cctgggtata taacaacacg cgcagcagca tcttgttgat gatcctcctg
     4381 cattcatcca gtaacgcagc gatctccgta ggtgcgcgag tcctgccaac ggagctttcg
     4441 ccagctttgc ataacttcgt gtacagcgga tggataccgg ccatcatggg cggcgtgatc
     4501 gcgatcctga tcctgatatt taccagaggt accctcggtt actcgaaagc cgtttcgcga
     4561 tcatccttgt gagaagagac aaagatcatg gcactacttc aagcctcgat cgtgaacagc
     4621 aaaacattcg acagcgcagg gtctgcgtcc gcccctgcca tcgtattggt acacggctct
     4681 gtggtcacac gcaagatgtg gctgccgcaa ttgcgcggtc tctcggatac attccacgtt
     4741 attgcaccag acctgcctgg acatggcgcg ttggcagaga tgccattcac tttcgccaca
     4801 gccgcgcaaa atctcgcgga tgtgatacgc cacgaggcga acggtcgtgc cctcgtggcc
     4861 ggactctcac ttggtggtta cattgctcta gagttggtgc gtcgctcccc cgaacttgtg
     4921 tcgggacttg tcctctcagg atgtagtctt aacttcgatg gatttcttgg ctcctacctg
     4981 aaattcgcca gccagttgat gaaacgcggg tggattaagt taggcaagga aaaatccgaa
     5041 gcgcgcgtca ggcgcatgtt cccacctgca ttagcagatg tggcagagga gcaaatacgt
     5101 gcgggcgtct atcctgatgc actggctccg tcatacatag aaatggcaca tagagatttc
     5161 agcgctgcgc tcgctgaata tccgggaccc agcctgatcc ttaacggcga aagagactcg
     5221 tcatctagaa agggtgaagt gaaattcctc accgctgctc aacagggacg tgtacaaaca
     5281 atcttagacg caggtcatgc ctgcaatctc gaccagcccg agagatacaa tcaagcagtg
     5341 cgggaatttt cacagtccat tgggtggacg tttgctcaaa ccggctgagc ctgtcacttt
     5401 aggagatatc cccattcgat aacgccagtt caatggcttg ctccaaagca agcgatcgtc
     5461 cagcatccca ttctgcggtg acagtagatt ctgcaagcac ctggcgggcg ggttcgatca
     5521 tcttgtcaac gcgcgcctgt tcagcgggcg gcaatggcgc accgatcgat tcgcgtagtg
     5581 ctgcggcaaa gccagctagc tggagggctc tttgagcttt cgcttctgcc gcagccaacc
     5641 cggcatagtc ttcaatcagg taggctattg ctgtctgatc gccaagctca cggttgatgg
     5701 ttagactttc atccaacagc gggcgagcat caccaaattc accttcatcc agaacaacgt
     5761 ctgctaatgt attgagagag agtgccagcc cggccttatc tcccaacttg cggcggatca
     5821 tcaagctttc ttcgagaaga acacgagcct ctgcatattc cttttggtcg ctggccacac
     5881 aggcctggtt gttgaggagt tgccccaccg cccattgatc gcccatggct cgaaagagcg
     5941 ccaggctctc ctcattcatc tcacgtgcac tggtcaagtc acgctggaaa cgggcaatga
     6001 ttcccaggtt attgaggaga gttgctacac caggggtatc gcctagttca cggcggagcg
     6061 ccaagctttc cgagttcaat tcacgagcag tggcgtaatc cccctgccac gtggcgactg
     6121 tccctgcacc cttgagcgcg tgtgcgcgcg ctttttgacg aaggcgcgta tctgcaatac
     6181 ctgccgagag attcaggctt tcgtcgtaat aggctcgcgc ttccgcgaat tttccctgca
     6241 gccgatagat ctcgccgata tcagccaata cattactcac acctgcactg tcacccaggc
     6301 gctcatatcc atcacgagcg cgcatgaggc gttcttccgc ggcgttgtaa tcgccgcgtt
     6361 ttctcatcaa ccaaccctgg gcgcgtcccg cctgtgtttg actttgcgca tcattgattt
     6421 gagttgccag tctctccgct tctcgaatga tctcctcggc attttcccac tcgcctatta
     6481 attcgaatac ctgacccagt ttgagcaatc caccgactcg ctcagcttct ggcaataaat
     6541 gcagtacgcg ctgatagtat gtaattgcgg ctgtgtttgc atagtctgcc tgagcagctt
     6601 ccgcagcctt caataaatat tcgcgcttct ttggctggtt gtcactgcgc tcgaagtgat
     6661 aggctaaaag atcagtgtat tgatcgagcg tatcttcata gatcttttcg ataaacttcc
     6721 cgatctggtc atggagcatg gcgcgggtgg caaatgataa tgtttcatat gctacctcct
     6781 gtgtcagaat gtgcttgaag agataagcca gttccggctc gggcgtatcc agcggcgtca
     6841 catccagcgc gctgagcgca tccaggtcac ttcgtacgcg ttcctggtca ccgaactgag
     6901 tataaactcc ccataaaatg gcgacgcgga acaagcgtcc gatcacactg gcgactttca
     6961 gaagcgtctt ctggttctcc gacagttgat cgatacgact gaggatcagg ctgtgaagac
     7021 tatcgggcag gtcgatctgt tcgagcgcct ctggttgttc cgggtcgatg tttcgatcgt
     7081 gcaggtagtt aagcaactcc tcgatataaa acggattgcc tcccgctcgg gtgctgatct
     7141 tctcgataag tgcctgaggc agttttgcct gctttccata aatgcgccga agcttaaaca
     7201 aaacaagttg ttcgatctct tctgcgggca aatcagctaa cgggatcgct gtgaaatgga
     7261 aaagcttgct gacgcgcgga gcaaggagga cgctgagttg aggaggtcga taggcgatcg
     7321 cgataaatat cgggaggtca acaatttgtc tgccaattac ttccagcaaa tcatgcgaga
     7381 gagtatccag ccagtgacaa tcttcgagca caagaaagag cggcatcgaa cgagctcgtt
     7441 ttcgcaaaca atcgaccagc agcgattcca acgactcttt acgcaacttg gcatcgaagg
     7501 aagaagtcaa ctcgttatcg gggatgggaa gattcagaac tgctccaagc agtggcaagc
     7561 gtggcagaag ccgtgggtca atctcagata actgcctctc caacgattcg atttgttcgg
     7621 ctacagaatc atttggatcc agatcgaaga aaccgcgcca gatattttgc cagacgaggt
     7681 aactggtact cgtcccatac gattggcact ccccaccgta ccccagcacg ttacgccttc
     7741 ttgcatgtcg aatcacttct gccatcaggc gtgattttcc catgccggct tccgcggtaa
     7801 tccccacaat ctgaccatgg cctctaaaag cgatatccag cttttgttca attaacgcca
     7861 gctcggttcg gcgtcctacc atcggaaggg catactttgg ctcgtgcaac cgaattccat
     7921 gatgttcctg attcgagatc agtcggaaca cagtaacggg ttcagatttc cccttgacgc
     7981 gtaatggagg caatccctcc cagataaatt cttctccagt cgctttgcga acattatggt
     8041 tcaccagagc ctgtccaacg ggagcgttct gcatgaggcg ggcggccata ttgaccgcgt
     8101 cgccaattgc accataagtt cgacgatata tcccaccaca agcgccagtt cgagagagtc
     8161 cctggctgac gccaatctgt atatcggtaa tgaaatccat ctgcaaatca cgcaagtcca
     8221 gggcagccct tactgctcgg atggcatcat cttcatgagc gacaggcgca ccaaaggaaa
     8281 catagaaatt actgcctttg tcgccaatga tcaactgaag catgtagctt tgatagcgcg
     8341 ccagaatctg ttgaactgcc tgtagataag cattgagctt cgattgcgct tcggggtcgt
     8401 catcataatc aattccggag aatcgcagga ataaagccac agtgggtcgt agttcggtca
     8461 ggaattcccc catgccgctc attagtcggt catagacagg tggcaataac cagggacgaa
     8521 gcagatcagc attgagtgca ttcgtatcca gcgccggcca gggatcgggc tccacatctg
     8581 tcttcaagtg atcaactact gcaaagcgtt ccccggattc cgaatcttcc cgccattcca
     8641 acacatcgat ttgatcactc aacgcggtga ccgcggcctg atcgagcaag acttcgccgc
     8701 gattggcatg atgttctgca gtcgctaggc gatacagagt cttgcctgcc aatgcatcta
     8761 ccaactgtat ggtcgggtcg ccgatcagga aacgacgcgc gggaccggcc gtcacgacga
     8821 ctttgatcgc cagagagaca tttcccgcac ctccgatatc cacctgggca aaccgccgca
     8881 tagtttgctg gatcgccatt gcagaagctg ccgcgcgccg tatggaagac gttttgggtt
     8941 gagaatggag caataattcg ttttggtcat caaaccaaca cgtgatggca tcgccgctga
     9001 aaccaattac actcccctgg tatctctcga tatcctccaa cagcgcatca tagatttcat
     9061 ttagccagcg tgttaattcc tccgcaccgc gttgaggacc taacacctgc accagagcct
     9121 ccgtcagtgg agtaaaaccg gatatatccg cgaagagtgc ggcacctgtc gtgtggtcag
     9181 gtaggtcgta gccgcgcgcc agtgcaaaac gcctgtctgt aggaatatac gcgtataaag
     9241 cttccataat aaatggcttt agctcaccgg cggctgaccg ctgcaagttg tctcataggt
     9301 accatatcgt caatcgtgat attttactat caacggcagg cttctctggc agctacctta
     9361 accgaataac gtagcattaa ggttacaact ctgggtattg atttattaag tatcaagcag
     9421 atagagaatt gattttttaa ttcgtcagat aatacggaag aacgcttgtg ggaatctgat
     9481 tcccacaagc gtctcagtga gccttctagc ttctacttga acttgaaaag tagtgtgtag
     9541 gactgcccat caatgaattc cacagtgaat agtcggcagg tgcccgccca cgatttttca
     9601 gttttccaga catagttata cgtattggtc gctgcatcat aggtaagcga gcttgaacca
     9661 gccgtggagg tttgctcgac ttcgtcgatc agtgccaggt ttgtacattg gacctgttgt
     9721 gatagcgggt agccggcttc gaagatagcc agcccctggt tcccgtcaag acggaatttg
     9781 atggggacgg agcgacccgc attcatctgg tttatcacag gtggattatc gaccgggctg
     9841 aagaaacccg tgaaattgaa gatgacgcgg tattcagcat tcgctacggc ggtgttaccg
     9901 gcattatctg tcgcagaaca ggtaaccgtc ttgctgccaa cggtgtttgt gacgactgcc
     9961 ccacagcttt gcgacgcaat tcctgagcct gtatctgtag cattggcaga ggcaatcgcg
    10021 gttccattga gcaggaccgg gttgggcgag acgctcggtg ccaaggtcgg gccggtctta
    10081 tcgatcttga tcggaccaaa gctggtgctg gcagaattac ctaaattatc agtggctgta
    10141 cccgtagcgg taaatgtgcc atccacgctg aagatctggt ctgccgggca ggaggcgatg
    10201 ccactttcgg tgtcactgca ggtgaaatga actgtcaccg tttgattcgt ccacgtgtcg
    10261 gctgtatagg ctgtggtgtc agctttggtc gcagtagccg taatagtcgg cagcgttgta
    10321 tcacccacga ttgtgagcga cgagccgaga tcgctcgagc tttcgtagta gtcgtcactc
    10381 aacgtcaggc tggggctgac cgggatcgtc ccattgaaat aagcatccac cgtatatgtg
    10441 cctggaggca atggcacgga cccgagcgta gcgttgcctg cgaaatctgc aatcacggat
    10501 gtggcaaacg aatccgtgct gttatgtacg ataaagaata tagatttgcc acccaatgct
    10561 cgcccgtcag aatcgcgcac caccgcaaca aagggcgtag cctgattggt aaagatgctt
    10621 gccgaagccg gtgtgacggt caaggtagtg gagtctttat tgaccgtaaa gtcgctaacg
    10681 tcattcgatc ccgaatattg agcattgcca ccaaagcgag cctgagcatt atagttaccg
    10741 ggtcgcacga ccagattcaa agtaagtgtt gcttctccgg aggaatcagt gagggcagaa
    10801 gcctgttgcc cgccgatatc aatgctcaca agctggttcg ccagaggctg cccgcctgat
    10861 tgcaataaca ggtcaaagct gctctccttg aggtaggttc cactcgcggg aggggattgc
    10921 agcgtcaggg tcgtccccgc gggaggcgga agctgggtcg cgttctcatc gcccacaccg
    10981 tagtatgcac caagattggt tgccagggtc gttagacctg caccgccgac tgcctgcacc
    11041 atgaagagaa tatcctctgc attttcacct gactgaagtg tgagagtgcc ttcccataac
    11101 gtggggtcat tcgtattttg agtcagatcc agaggtaccc aggttccatg atagctgctg
    11161 ccgggcttac cggtgtaaag cacccatacg gcctgaactc ctgcagagcc atccgttgcg
    11221 gcgttcacac tgaaggcgat cgtgttgcca ttctcctcgc ccgatgctcc cagaatcacc
    11281 ggagaggcac taacgcccgc ggctttggtt tcagggctgc cagcattggt ccaatcggca
    11341 gagagatagt agagttccaa atccaactga ttgaatttgc gcaggatgcc atcaattgca
    11401 ttcggcgcgc ttgactggaa ttgggcgggg aagaggatca atcgcgtttg gccgccgctg
    11461 attgcatcgg agaagttcga tgcccaggtc tgcgttgggt agaaaacatt tgtgttgaag
    11521 gaaagatgtg cggttgatgt ttctgtggtc ggcgaagagg ttagcgggac aatgccagcc
    11581 aggtctgtat aggtaccacc acgcagtgcc acgcctcgca atacatttcc ggaggaagtg
    11641 acgttgaaga tctcctttgg atagatcggc tcaaatgggt tgacaacgac accgtccgcg
    11701 ccggagagat acgtggtcgt aaccgttcca ccaccagaca gattttgaag gaccttgttg
    11761 tgggtattga caaccgggtt cagcaccaca gggctacgcc gcaggccgaa gtttgcgcca
    11821 ggcgtcgcac cgacagggct ggttgagccg ataatggagt tatccgctgg cggattgagg
    11881 cgcgcaccag gcatgttcac tttcatcatt ggcaaaccgt aaagagtcat ctcgaccaga
    11941 gtcttatgat cgatgccagt taattgtgcg gtctctgcga gatattgctg tttggctttt
    12001 acgacagcct gccccactgc aacgggatca tttcctgttc gcagttgttg tgccatgagc
    12061 aggaagaggc gctcgccata ttcgactaat tctgtatcgc cgtaagcata tcctgtagcg
    12121 gatatatagc cggccgcgcc atttgccagg aaggccttcg cccagtccgg gtttggagag
    12181 atgccattca gcaagtcact atttggaatg ctatacccac cgtgacatcc cagagcaagg
    12241 aacagaacat cggtcatatc ggtcattgca gcgatctctg ttgcagagag ttcggttgcg
    12301 taatccgctg cgagcaaatt acctgcgctg aagtgaccgg tcagcacggc gatatcaaag
    12361 ttgccgccct gtagcttcga gcgaagttga ttggccgtcc aggcactggc gtcagtggga
    12421 ggcaggccgg gtgcttgaat caatgtatcg gcagtggagt ccgtgcccgc gtccatttct
    12481 gcttggatcg ccacagctgc gtcgccgaca aagtcatagc ccgtgaccag gcttgaagta
    12541 ggctcaatta tgccgtcggt ctcgatatag gcatcgatgg cggtactgat ttcactggct
    12601 gtgtccacta agcgcccaac cgctagatca ggcagagcca atgtatagcc agcttgcgta
    12661 aagtcgatct gtgagccgta ggcatcctgt ccctggacga ggttggtctt cagcccagct
    12721 tcggaagccg tggatggcgc gactggaaca acatattctt tttcgaaagc gagaccagag
    12781 acatcctgca cctggaagaa cgggatgaca tccgcgccac cagctaacac gatgtatttg
    12841 agagtcggat ttgcatcgcg gtattcgtcg atcacttgct tgatctcttt tgcgaccgtg
    12901 ttcttggcgg ctgcacaagc caggttggcg tctgcttcgg catttgcaaa tgcgacgcgt
    12961 tcatactgag catcactcaa atcaatcaca acaccgttta catcggagcg tgcggcgagc
    13021 acatcaatgt catccaaggc ggctgcaatt tcgccgggtg taccgtgcag gcgggcggag
    13081 tcggtcagaa ggagcgtctc atgggagccg gccgagggcg tcccagcaat gacctggagt
    13141 ccactcggga cgacatccac atcgccacag actccacctt ctacaaacac atccaaagta
    13201 aacggagttt caaggtcata tggtcctacg acacgcacat aaagatcttc ctgaaggtca
    13261 tacgtgttgc gttcgatcgt ttggaccgtg gcgtacggat ccatggaaat tcccattaag
    13321 ctgttgatcg cggcactgcc ataagcatcc agcgaaccag agggtaatga gccggagggt
    13381 aacgaacccg aaggcaatga accagatggg agtgaaccag aaggtaatga acccgatggc
    13441 aatgaaccag atgggagtga acccgaaggc aatgaaccgg aaggtagcga acccgaagga
    13501 agcgaaccag agggcaatga accggaaggt agcgaacccg aaggaagaga accagaaggc
    13561 agtgagcccg aaggtaatga accggaaggc agataaccgg ttggaagtga accagatggc
    13621 agtgagccag atggcagtga ccccgaaggc aatgaaccgg aaggcagcga acccgagggc
    13681 aaagagccgg agggcaggaa cgcggcatcg gatgcctccg cactcagcaa cgcggcattt
    13741 tgcggatcga tcagcgcgtt atagattggg tgcgggtcgc gatgtaagct gaccgcgctg
    13801 cccggcaaac tcgtcaactt gatgttgaca gttgcacccg gctgaaccgg gaacttgaac
    13861 catttttcct ggaaaacatc cgtgatgaat tgttgagttt tcgaatcatc tgaaacggat
    13921 tgcgcctgtg cccaatttag attcggcgta gcaaccggac gacaatagga gatttcagat
    13981 gtgccattat tgcctgtgac ggtcgcgctg atcccgaacg gttcatcagc tccagccgac
    14041 agattctggt caaagacagc cacgccgctc gcgtccgtgg acacgacgaa ggaacccagg
    14101 aaagattcgc tttcaccaaa gaatgtggga tcacaggatt cattggcgaa tacatcaatg
    14161 ctgtaattct gattcgcctc tgattcgagg taaccaccca cacgcaatga ttcgccatct
    14221 gaggtagcca cgctcataac cggaaagttc tggaaattgt tcggcccagc gacgaaacca
    14281 acgtgattga atgtaacgcc gtcaccggcc agatcaatgc cgagtccgga attggagaaa
    14341 atattatttc cgcggatgtc ggtgccggtg gcagcgccca cctggacacc tgcgccagta
    14401 ttgaacgcaa tcgtgttagc agaaacgcgt ccgatgccgc caatcgtatt attcgggccc
    14461 gccacgtaaa tacctgcggt ggcgctcgtc ccggctaacc catcatcacc ggagtcgtta
    14521 ttcccgttgg gcaacgggtc agtgccattt gcattcaggc cgatcaagtt tccggtcaca
    14581 aagtttccgg tcacgtttga atcgcgcaat aagattccaa aacgaccgtt tccggaaatc
    14641 acgttgcctg agatgctgtt ctctgttgcc gtctgtccaa aagacccgac aaagataccg
    14701 cgctggctat tccccatcgc ggatgtgcct gctgggttcg ttccaatgta gttgcctgtg
    14761 ataacgttat tggtcgcggg gcccgcgcca gcggcaatga aaatcccatc ccggttggca
    14821 gaaatcacat ttcgagccgc gggtgtcgaa ccgccaatta tgttgtctga tgtgttgttc
    14881 agatatatac ctgaagcgac aactccatcc gctgtgccat tccctccgag tccgaagaaa
    14941 ttcccggtga tcgtatttcc ggatccattt acttcgatac cattattgaa gttcccatga
    15001 atcgataagc ctgtgatggt gcttccaccc gaactaagca ataacccctg cccacagagt
    15061 ccatcacagc tatcgcctag gagttccacc cggatcacag cattgttacc gacagacagt
    15121 gtgttttggc ttgcaccaaa ctgtgtgtaa ccgtcaataa ccacagcctc cgtgatagca
    15181 ggcaaaggcg tggccggaga aatgatattt atgccctcac tggggatatt gaagtgaatt
    15241 tcgtctgcgc cattgaccga atttggcaag gcgttggcgt ctacaatcgc ctgacgcaat
    15301 gacccttcac cgctgtcgtt tgtatttgtc acaaccaaaa tgttggtgaa gacggattca
    15361 aacgcaccga tatcgcaggt ttcaccctgt gggcgtgtga caccgcgttg atcggttgag
    15421 gcgcagctct cgatgtcacc tgcgtcaata gccgggctat ccgtcagcaa tgccatggtt
    15481 tcagtagacc caccgttgtc agccaaggta tcgagaagtg gatcggcctg agtacccgga
    15541 cagttatttt caatgggcca gctcaggtta ttgcccagat cggttacggt ttcacaatta
    15601 ttgagtccat ctccggacaa aatcgaatta ctgactcgta gttcgccgcc ctgaccacgc
    15661 tgcgccccca atgtgtcccc gctaccagag gctctgttcc ctgaaaaggt gctattgttt
    15721 acgatgatcg tgccgacgcg tgaatcgatc gcgccgtggt tgaccgcttc gttatcaaca
    15781 aatgtgctgt tggttacttc tacaaaatcg gaattgctat ggatcgctcc gccggtgtcg
    15841 gcgctatttc cgaaaaatgt actattgttt actgtaactg aaccaccatt gggatacagt
    15901 gcaccacccc cgaaaaccga tgagttcaaa tcgaaggtgc tatcggtaat cgtaaatgaa
    15961 ctggcatacg tatttgatac cgcgccgcca ccaaaatggg caaagttatt cgtgaagcga
    16021 ctttctgaaa tgtccgctga ggcttcgacg gtcacgtgga tcgcgccgcc ggtgccattt
    16081 gaatcggtcg aggtggcgcg gttatcatca aaggtaactc ggttgatgtt tacgtgaccg
    16141 ttcaccagga tcgctccacc cgggcttgcc gtaccatttt tgatcgtcag ctcagataac
    16201 tccacgcccc aggactgaac ttgaaatgca cggtcgattc cattggcatc gatgaccgtt
    16261 gctcccatcc cagcgccgac aatggtcaca ttatcgaaga tgtccaggtc gccagtcgca
    16321 gcagcgtttt catctgcccc ttcaatcgtt aatgtgtata cgccagcggg aattgatatc
    16381 gtgttcgccc catctcttgc gttggcttct tgaactgctg cacgcagtgt acagtcacca
    16441 gtaatatccg tctcacagag gccgtcgccc gggaaggcat ctaccatatc tgcagtacta
    16501 ttgactgtaa agtctgcttc agccaaggct ggccgaacgc cgaattgtgc gagcagaatg
    16561 gtaagaatga gaaagatgta agtaatgcga gcgagctgtt tcaacatgat ttacctccgg
    16621 gtaaaaaggg acattgcctg gtcattaagt cctggtgaat aacttgtgta ttggagcaac
    16681 ttgccttgca ggaagcaatc gttcaaagag gtgggctgcg gctttagagc agcaaatcct
    16741 aagaattcat ctttaatgat cgaccccttg ttagcccagg taactcttgc cttaaaaagt
    16801 catctctcca ggtggatcta tacccagctc accatgtact ggaccgatca acggagaatt
    16861 cgtctacaag acggcttctt gttgagtcgg atatacaatt tcgttaagat ataatgaaat
    16921 tgcaatttag attatgggct taacccggtg cacgttctgc aactagtgga gattgagcaa
    16981 caatttttag gaaaaagcca tgccaaatgc tcgctggacg gctgaaaagg caaacgaatg
    17041 gtatgaaggc ctaccctggc tggtagggtg taatttcatc cccagcactg ccatcaacca
    17101 actggagatg tggcagccag agaccttcga ccctgagaca atttcgagag agctgaaatg
    17161 ggcagcaggg attgggttta atgtggtccg ggtttatttg cataatctgt tgtgggaatc
    17221 tgatcaggtg ggtttcgagg aaaggataaa aagatttctg gatatcgcag ctagtcatca
    17281 gatccgtact gtatttgtgc tgttcgacga ctgctggaac acaaatccgc aattgggcaa
    17341 acaacctgag ccgaagcccg gaatccataa ttcgggatgg gtgcaaagcc caggatcaaa
    17401 actggtgaca gatgaatcaa gctgggacac tctagcggat tatgtgcgcg gtgtgatccg
    17461 cacgttcgct caggatgagc gcatcctgat gtgggatttg tataacgagc ccgggaataa
    17521 tggacttgac gagaagtcct tgccgctatt acaaagcgct gtgacctggg caagagaggc
    17581 aaatccatcc caaccaatca caattggact ctggtacgaa aacgaatcgt tgaacgactt
    17641 ccagctttac gcctctgacg taattacttt tcacaactat ctgcccgaag acgatttatt
    17701 gaagcagatc acgacgctaa aagaacatgg cagacctttg atatgcacgg agtatatggc
    17761 tcgcactcgc gggagtttat ttgaaaagtc actgccaatt tttaaaagag aaaatgttgg
    17821 gtgtattaat tggggactgg tgaatggaaa gactcagacc atttatccct ggggatcgcc
    17881 tggcggtgaa gccgagccag agttatggtt ccacgatatc tttcaccgcg atggtaaacc
    17941 ttatcgacag gaagaagtcg attttattaa agagataaca ggatgatagg cgtatgtgtt
    18001 aatccacaag gaataacctt acaacccgga aagagctcat tgaggtatca ac
//
LOCUS       DASTJP010000346         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_912886, whole genome shotgun sequence.
ACCESSION   DASTJP010000346 DASTJP010000000
VERSION     DASTJP010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912886"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /gene="add"
                     /locus_tag="VFQ23_10355"
     CDS             <1..822
                     /gene="add"
                     /locus_tag="VFQ23_10355"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET9907036.1"
                     /translation="LKESEALRRTALEAAEDAHHDGVIYFELRAGPALHARPGLPIEE
                     VIESILAGLEEAEAKYGIVTGFIVAGLRNHDPHINTQLAQIACRYMDHGVVGFDLAGD
                     EARFPAELHQESFRVVQESGLPYTVHAGEASGAENVLYAIEALGAIRIGHGVRSIESR
                     AVMELLRERQVLLEVCPTSNVHTGTVPTIQAHPVKALYEYGIPISINDDDPITSRTRV
                     SNELMLLHTMFDMPVESLVKIQLDTLDHCFLRDKEVVARLKSTVQVFASGLPSIT"
     gene            874..>2134
                     /locus_tag="VFQ23_10360"
     CDS             874..>2134
                     /locus_tag="VFQ23_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9907037.1"
                     /translation="MITQPKLDTNEVAEFITHLMVRFSEAGEFSAQLPEILQEAGEFL
                     NAQRITMSFQSRGASTQEISWVSNGGAGDESAFMFHARLPKSGVKNSIHFYMPTSGSV
                     QEPVYQLIATLIDNQLATGQTIQAEHSQRLLAESINQISKILTSTLDREELLSLFLDQ
                     LETLVPYDTANVMVLQDGLLYMHAARGYGEFSAPVDISKITFIPNKTFLMNEVLMGSQ
                     PVILNDTHNSPQWTWAPCGEHIRSWMGVPLRVKGNAIGLFSIDKSIPNFFTEKHAQLA
                     SALSKHAALVLDNALMFSQLQDAHTQLRGLSVRITRAQEKERKKIAMELHDHAGQALL
                     ALRAELQVLRHNLTKSPDKGQEQIDYLDQIVLDLNRDLEQLAYDLRPPTLNALGLISA
                     LKQYISDFGQRMQIEADFIYDTDIPRLS"
BASE COUNT          541 a          520 c          533 g          540 t
ORIGIN      
        1 ctcaaagaaa gtgaagcact gcgtcgaacg gcgttggagg cggcagaaga tgcccatcac
       61 gacggtgtca tttatttcga acttcgagca ggcccggccc tgcatgctcg cccgggtctt
      121 cccattgaag aagttattga gagcatactg gccggcttgg aagaagctga agcgaagtat
      181 ggaatcgtaa ccggctttat tgtggcagga ttacgcaatc atgaccccca cattaacacc
      241 cagttagcgc aaatagcttg ccgatatatg gaccacggtg tagtcggatt tgatctggca
      301 ggtgatgaag cgaggtttcc ggctgaactt caccaggaat cgtttagagt ggttcaggaa
      361 tcaggcttgc cctatacggt tcacgccggc gaagcctcag gggcggaaaa tgtactctat
      421 gcaatagagg cccttggcgc catccggatt ggtcatggtg tacggagcat cgaatccagg
      481 gcagtcatgg aactcctccg cgaacgccag gttttgctgg aagtttgtcc taccagtaat
      541 gtccacacgg gtacagttcc aacgattcaa gcccatcctg taaaagcatt atatgagtac
      601 ggcattccta tctccattaa tgatgatgat cctatcacct cgcgcactag ggtttcgaat
      661 gaattaatgt tgttgcacac tatgtttgac atgccggtcg aatcgctggt taagatacaa
      721 ttagacaccc tggatcactg ctttcttcgg gacaaagagg ttgtagcccg cttgaaatcc
      781 acagtgcagg tctttgcttc cgggcttccg tccattactt gatggctgga catgcgatct
      841 gcaaaccgag actctacaat tgggacaagt gcattgatca cccaacctaa gcttgacacc
      901 aatgaagttg cagaatttat cactcacctt atggtccgat tttcggaagc aggtgaattt
      961 tctgcacaac ttccggagat tcttcaagag gcgggagagt ttttgaatgc gcagcgcatc
     1021 actatgtctt ttcaatctcg cggtgccagt acacaggaga tttcatgggt cagtaatggg
     1081 ggagccggcg atgagagtgc attcatgttc catgcacgcc tgccaaaatc cggtgttaag
     1141 aatagtattc atttttatat gcccacttcc ggtagcgtgc aggagcccgt ttaccagtta
     1201 atcgcaacgc tgatcgataa ccagctggcg acaggccaga cgatacaagc ggaacactca
     1261 cagagacttc tcgctgaatc gattaaccag atttccaaaa ttctgacaag cacattggac
     1321 cgagaggaat tgctttcact gttccttgac cagcttgaaa cattggtgcc gtatgacacg
     1381 gccaatgtta tggttctgca ggatgggctt ttatatatgc acgctgcgcg tggatatggg
     1441 gaattctcag cgccggtgga tatctccaag attaccttta ttcctaataa aactttcttg
     1501 atgaatgaag tactgatggg aagccagccg gtaatcctga atgatactca taactcgccg
     1561 cagtggacct gggcgccctg tggagaacat attcgttcct ggatgggcgt tcctctgcgt
     1621 gtcaagggca atgccattgg tttgttttcg atcgataagt ccatcccaaa cttcttcaca
     1681 gaaaagcatg ctcagctagc ctcggcgctt tctaagcatg ccgcccttgt gttggataac
     1741 gccctcatgt tctcgcaact ccaggatgcc catactcagt tgcggggact ctccgtgaga
     1801 ataaccaggg cacaggaaaa agagcggaaa aaaatcgcca tggaattaca tgaccacgcg
     1861 gggcaggcct tgcttgcact gcgagccgag ctccaagtat tacgccataa cctgacgaaa
     1921 agtccggata agggacagga gcagatagac tatttggatc agattgtgtt ggacctcaat
     1981 cgagatcttg agcaattagc gtacgattta cgtccgccta cgctcaatgc gctgggtctc
     2041 atctccgccc tcaaacaata tatttccgat tttgggcagc gcatgcaaat tgaagctgat
     2101 tttatatatg acaccgatat tccacgattg tccg
//
LOCUS       DASTJP010000347         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_750483, whole genome shotgun sequence.
ACCESSION   DASTJP010000347 DASTJP010000000
VERSION     DASTJP010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750483"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(770..1756)
                     /locus_tag="VFQ23_10365"
     CDS             complement(770..1756)
                     /locus_tag="VFQ23_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907038.1"
                     /translation="MNSPKSNDDLVFNPRTLRLIIGALAFAFPITVIALTGKITTSIS
                     ASYYEDANRDVFVGFLFIMGALLIAYKGHLHGDTKPHGETALHWLFSFKWFKVYQEEI
                     ISTFGGLAAIFTAIFPTACDNCTITTEARIHAIGACIMFAAVVYFTLFAFLRSVNIKL
                     VEDNPAFESLANRYTNQLFGSISIFRGIIKRVRMDFDNKNGVQVNRALQNNGDEINPQ
                     SLFARLLAEARTNKLLFLWFAYERKISRGIVYLICGPAITITLLLFLYISWTRPDLVA
                     NSTITFKVETIALLFFGIAWMTASKLGYLKQILSWLPGGSRKTSSESLANAA"
     gene            complement(1796..2146)
                     /locus_tag="VFQ23_10370"
     CDS             complement(1796..2146)
                     /locus_tag="VFQ23_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907039.1"
                     /translation="MPNPYFNIMPNQVAGAAPPSAISVPQATLTFAGAPAAVTIVVKV
                     LSVAFPSSTLATNPQTLLVGLSLLVGLLIYWSTAPTGQNTKDKVLGVVFALINSFAIA
                     AATLGIETAAVLIK"
     gene            2468..2623
                     /locus_tag="VFQ23_10375"
     CDS             2468..2623
                     /locus_tag="VFQ23_10375"
                     /inference="COORDINATES: protein motif:HMM:NF042904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6799 domain-containing protein"
                     /protein_id="HET9907040.1"
                     /translation="MVIEQGLLIMTHEQVILLKNGELVILTEDLTLPDGTRVALDGSV
                     TMANGTL"
     gene            2962..>4189
                     /locus_tag="VFQ23_10380"
     CDS             2962..>4189
                     /locus_tag="VFQ23_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907041.1"
                     /translation="MLLKETGADLPVDVRSRVEALLSLVSDISKRMVQFPVLFDLIQD
                     FHEMAKAYILLFEAKLKVADTMNPRDKWLKQHEMLDKLTKEWHLIYSLAAATELGSSF
                     LGKFQPYVDQAASDIGLTDTRGDFLLIPNFGESFSLVTVGYSSSNIAILNLPISVIHS
                     PWELSVIWHELAGLKVIKIREQIKNFLETYARENHLELPNSRKTSGNDLIAELFRRIT
                     EGRRLDKTFKLEVKKYLSSGNGAVQKQEEIWSQDWFEQLYEDACSVFAFGEEFVSVLK
                     TILGRQARKLTADRKHPDLGTRIQVAKRLLALQKGSAPAPVTATDRLTDNLLWAFLNQ
                     KRTDLVSALPVAFLDPAGLPEVRRELVNQMQIFNETFGDLEGGTIHVTPFEFGSMYLL
                     EQKASDRPTRIQEKLLT"
BASE COUNT         1097 a          925 c         1102 g         1065 t
ORIGIN      
        1 atgaagagaa aattcagcga tggaaaatcc ataaggatat tacgtttatg taggaatcaa
       61 aagtgattcg ttggtcgagt gccgaaggcg taccgagacc aaagggaacc atgataacaa
      121 aagatgtctt ggaaagttgt ggtctcgata cgcccctccg cttacactcg ggactactcg
      181 accaccggac ctggctagga atctaaaact gtcagatggt ttgaaactga ttacaccgac
      241 aggatataaa atggctttcc tattcgcttc gccaatagat catgatccag agtttccttt
      301 acggatgggt tcgaccttgc gaattataaa cgcaggaaac catctctgcc ccacccatcc
      361 taccgctcgc tttgcttggg tacgctgcgc agtccccaaa tacgacaacg aaacatcctc
      421 ttcacattta atatttctcc gtcggatttg gggagggaag acttagtgac cctggcaacc
      481 cataatttca actagttccc ggcggttgag tgcctaacgc gtgcgcctgc aagagtgctt
      541 gatttttgaa ttgccgggca attcatagca aaacatgcag gtgtcgagac cacaaatctt
      601 ccggtaatgc tttatttgaa ggctatctgt ggtctcgata cgtcccgcaa aggcatcggg
      661 actactcgac caccgagggc tagttggtat tgagaactaa tcacaattca aaaaaggtgg
      721 acggtaacgt ccacctttcc tgtcagaagg ttgatagatg aaagctcgtt caggcagcgt
      781 tcgccaggga ctctgaagat gttttcctgg agccgcccgg gagccagctc agaatttgtt
      841 ttaggtatcc cagtttgctg gcggtcatcc aggcaattcc gaagaagagc aaggcgatcg
      901 tttcgacctt gaacgtgatg gttgagtttg caaccagatc tggtcgtgtc catgatatat
      961 agaggaagag tagcagggta atggtaattg caggaccaca aatcaggtat acaatgccgc
     1021 gcgaaatctt cctttcgtac gcaaaccaaa ggaacagtaa cttattggtc cttgcttcag
     1081 ccagaagcct ggcaaatagg gattgcgggt tgatttcatc gccattgttt tgcagcgctc
     1141 ggttaacctg tacaccgttc ttgttgtcaa agtccatgcg cacccttttt atgatgccgc
     1201 gaaagatgga gatcgaaccg aacagttgat tcgtgtaacg atttgccagg gactcaaagg
     1261 ccgggttatc ttccactaac ttgatgttga cgctgcgcag gaacgcaaac agcgtgaaat
     1321 agacgactgc cgcgaacatg atgcatgcac caatggcgtg gatgcgagct tcggttgtga
     1381 tcgtgcagtt atcgcaggcg gtggggaaga tggcagtgaa gatggctgcc agaccgccga
     1441 acgtgctgat gatctcttcc tgataaacct taaaccattt gaaggagaat aaccagtgca
     1501 aagcggtttc accgtgcggt ttagtgtcgc cgtgtaaatg tcctttatag gcaatcaata
     1561 gagctcccat tatgaaaagg aagcccacaa atacatctcg attggcgtct tcataatagg
     1621 aggcactgat cgaggtcgtg attttcccgg tcagggcaat cacagttatg ggaaaggcaa
     1681 aagccaacgc gccaataatc aggcgcagcg tgcgcgggtt aaataccagg tcatcatttg
     1741 atttgggtga gttcattttc cactccttga aaaactctgc gggttgtccg ctctgttact
     1801 tgatcagcac tgctgctgtt tcgatcccca gcgttgccgc ggcaatggcg aaggagttga
     1861 tgagggcaaa cacaacgcca agcactttat ctttggtgtt ttggccggtt ggggccgtgc
     1921 tccaatagat cagcaaccca acaagtaacg ataagcctac cagcaaggtt tgggggttgg
     1981 tggccaaagt agagctggga aaggccacac tcaatacttt tacaacgatg gtgacggctg
     2041 ctggcgcgcc ggcaaacgtc agcgtagcct ggggcacact gattgcgctg ggaggcgcgg
     2101 cgcctgctac ttgattgggc atgatattga aataggggtt gggcattttt gttctcctct
     2161 tgggtatccc atattttgat gattcacttc aataaacgtg gttttggtaa tttttctggt
     2221 aagttctttg accccctccg tcccgcctac ggcggagaca ccttaagcga agcaatcctg
     2281 tggaccccaa atccgattgg aattcatcgg atttggggga gggagggtgg gggtagggta
     2341 cgtccgaaat tagtatgatt gcttatccta tctttcagag ggatagatac tggttgatta
     2401 tcaatggtcg ggtacgtaga atgaagttga agaataggat ctgataaaga aaagagagga
     2461 agcgagaatg gtcattgaac agggcctgct gatcatgaca cacgagcaag tgatcctttt
     2521 gaaaaacggt gaattggtca ttttgactga agacctgact ttgccagatg gtacgcgcgt
     2581 tgcattggat ggcagtgtga caatggcaaa tggtacgctt tagatgatcg gcgaaggtca
     2641 ggcagtcctg gtggatggct agcaggccaa catcaagggt ggatagttgc tgtaaagttc
     2701 ttattaaatt aagaagattg agcctataat tgaatccggc tggcagcgtt ctcaggcgcg
     2761 tcagtgggat ttttgattgt cgtatcctca tattcgcctt caggctgcgc ttcgcagtct
     2821 ctcatggaaa gaaggatgtt gcattggagc ggagcataac ggaaaaatcc ctcaggctgc
     2881 gttcataaat gtgaggtgaa aatgaataca caatttacat caatgtctga tccacacatt
     2941 cgtataaaag aggaggaaag tatgttgctc aaagaaacag gtgctgactt gccggtggat
     3001 gtacgttcac gcgtcgaggc gctgctctca ctcgtttcgg atataagcaa gaggatggtt
     3061 caattcccgg tcctgtttga cttgatccag gattttcatg aaatggcaaa agcctatatc
     3121 ttgctcttcg aggctaagtt gaaggttgcg gataccatga atccacggga caagtggctg
     3181 aaacagcatg aaatgctgga caagcttacg aaagaatggc acttgattta ttctcttgcc
     3241 gcggccacag aactagggag ttctttttta ggcaagttcc aaccctatgt ggatcaagcc
     3301 gcgtctgata ttggtttgac agatacccga ggagatttcc tgctgattcc aaattttggc
     3361 gagagcttct ctttagttac ggtgggttat tcttccagca acattgccat tctaaacctg
     3421 ccgatttcgg tgatccattc gccctgggaa ctcagcgtga tctggcacga actggctggc
     3481 ttgaaggtaa tcaagatccg cgagcagatc aagaatttcc tcgaaaccta tgcgagggag
     3541 aatcatcttg agctacccaa tagtcgaaaa acgtcgggga atgacctgat cgctgaattg
     3601 ttcagacgca tcactgaggg gcgaaggctg gataaaacct tcaagctgga agtaaagaaa
     3661 tatctttcct ccgggaatgg tgcagttcag aagcaagagg aaatctggtc acaggactgg
     3721 ttcgagcaat tgtatgaaga tgcctgcagc gtttttgcgt tcggtgagga attcgtatca
     3781 gtcttaaaaa cgattttagg tcgtcaagcc cgcaaactaa cagcggatcg gaaacaccct
     3841 gacctaggta ccagaatcca ggttgccaag cgcctgctgg cactgcaaaa gggaagcgct
     3901 cctgcaccag tcaccgctac ggaccgctta actgacaacc tgctttgggc ttttttgaac
     3961 cagaagagga ctgacttggt ctcagcactg cctgtggcct tcctggaccc tgcagggcta
     4021 ccggaagtcc ggcgagaatt ggtcaatcag atgcagattt ttaatgaaac ctttggggat
     4081 ttggaaggtg gcaccattca tgtaacacct tttgagtttg gttctatgta tctcctggaa
     4141 caaaaggcaa gtgacaggcc cacccgaatt caagagaaat tgcttacat
//
LOCUS       DASTJP010000348         5290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_855102, whole genome shotgun sequence.
ACCESSION   DASTJP010000348 DASTJP010000000
VERSION     DASTJP010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855102"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFQ23_10385"
     CDS             complement(<1..472)
                     /locus_tag="VFQ23_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET9907042.1"
                     /translation="MNQRPTGLSLSKATVGFLQYKSAEGLSPVTVSGYDHDLKLWIEY
                     QGDMDVAEVQTTHILAYLNHLRTDYVPRRITGNNDKKLSPKTVYDLYVSVASSFTWAS
                     REFQIDNPVKGIPRPRVPDGAPVEPFRKEEIELLIKACDFCEKAVTDRRHKFIMQ"
     gene            complement(564..635)
                     /locus_tag="VFQ23_10390"
     tRNA            complement(564..635)
                     /locus_tag="VFQ23_10390"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Met,seq:cat)
     gene            873..1529
                     /locus_tag="VFQ23_10395"
     CDS             873..1529
                     /locus_tag="VFQ23_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9907043.1"
                     /translation="MKNTTLLVIEGRHAEIPSFAPDLQKKGFDVVSFQNGSQAVSKLK
                     QTEASLVVINAASLRSTGIRICLSVRKKEPKTPIILIVENEKAVDKELADSVLVLPFT
                     VQKLVNRIKALMPGDGNNVINVGPIRLDVEHRRVKSLGKNTRLTPRLITLLQILMDKH
                     GEVVERESLFKKVWETNYTGDTRTLDVHISWLRRAIELDPDDPKFLKTIRGVGYRLDV
                     "
     gene            complement(1518..2741)
                     /locus_tag="VFQ23_10400"
     CDS             complement(1518..2741)
                     /locus_tag="VFQ23_10400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET9907044.1"
                     /translation="MPELTWHGKGIAQPAPAQLATDSIIYPNGYGYPLLDIENRLILG
                     DNLAVMSALLPEFEGKISLIYADPPFFTNRKFPARIGRGEDSRKPNKWKLVDGYHDAW
                     DDLDSYLDFLYQRLAVMYRLLEPNGTLYLHLDWHADSYARVLLDELFGRDHLLNEIIW
                     AYHGPSPIRRAFNRKHDTILAYVKGADYTFNVDAVREPYNKNTVKTFNSSPKAGFGKV
                     PDLKRGKVPEDWWYFPVVARLHNERTGYPTQKPVGLMERIILASSNPGNIVADFFCGS
                     GTTPFVAAKHGRRFIACDETFHALHTTRARLTETQAPFLLEHDSNFAFPTILAPNSVK
                     VNRLDHFISIESDLDLDYWEVDIDWDGTLFKSFAQARRPVRNGKIPHELKIKTGGNHC
                     IRFVTAQGEQFQLNV"
     gene            complement(2744..3781)
                     /locus_tag="VFQ23_10405"
     CDS             complement(2744..3781)
                     /locus_tag="VFQ23_10405"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9907045.1"
                     /translation="MASLSRRDFLKLGGLSLGSLGFSPALKNLSDFDDSNLVRIATNS
                     VSVYRRPTDESSIVSTWYRDELLRIYGEVEADEPSHNPIWYRVWGGYVHRSHLQRVKI
                     LLNKTLSALEDGTPRLVEITVPFTEPWRTSKAFGWQKLGFRLYYQSTHWVQAIEEGPT
                     GEPWYQINDDLAGLYHVNPLHVRPIPLAEIEAITPEIPLEKKRIEVNLTTQKFVAFEN
                     DREVFKTTIASGLPNGPRGADGLSTKTPQGEYRILEKDGSKHMGNGNLFADADDYELP
                     GVPWTCFFTTQGHAFHGTYWHENFGVPMSHGCINMRTEDAKWLYRWALPKHEMGRHAN
                     RGFGTLVQIYY"
     gene            complement(3907..4149)
                     /locus_tag="VFQ23_10410"
     CDS             complement(3907..4149)
                     /locus_tag="VFQ23_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907046.1"
                     /translation="MDYGMIGKREKARRYAEERNRFLFNKFDLTFHGDNNNHHVTFDN
                     GVYGCDCEFYVTHHRCAHTMALEILLQGMVPETIQS"
     gene            complement(4352..>5290)
                     /locus_tag="VFQ23_10415"
     CDS             complement(4352..>5290)
                     /locus_tag="VFQ23_10415"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9907047.1"
                     /translation="WHIYLLTGIQAAAMAFDLPARQALVPNLIPRDILPSAFSLQSIA
                     FNTGAVVGPAMSGIVIGYLGQEFAYFINAVTFLAVIFALIALGNVPQQQSKSQSGLRP
                     ALVDIRDGLRFIRRQPLILSTMILDFIATFFSSANTLLPYFAQHVIHVGEVAYGWLAA
                     AQSIGAVLVGLIVSQYNNIRRQGPLLLGAVVVFGLATILFGFSRWYALIFLALALAGA
                     ADSVSTIIRNTIRQLNTPDSMRGRMTGINQIFFMGGPQLGEIEAGAVAQLFGIPFAII
                     SGGVGTILGVWLVASIWPVLTQYNGDEPNAMTAPAD"
BASE COUNT         1448 a         1294 c         1241 g         1307 t
ORIGIN      
        1 gctgcataat gaacttatga cgccggtctg ttactgcttt ttcacagaag tcgcaagcct
       61 ttatcagcag ctcaatttcc tccttcctaa aaggctcgac gggcgcgccg tcaggtacac
      121 gtgggcgcgg aatgccttta accggattat cgatttgaaa ctcacgactt gcccaggtga
      181 aagacgaagc cacactgaca tagaggtcgt aaacagtttt tggagaaagc ttcttgtcgt
      241 tattgcccgt gatgcggcgt ggaacataat ccgtgcgcaa atgattcaag tacgccagga
      301 tgtgtgttgt ttgcacttcg gcaacatcca tgtccccctg atattcgatc cagagcttta
      361 gatcgtgatc atagccggat acggtgacgg gcgacaaacc ttcagcactt ttatactgaa
      421 ggaatcctac ggtcgccttg ctcaagctca agcctgtagg tcgctggttc atagaaaatc
      481 tccttttttg ggagactctc tgaatcaaaa accgccgggc tagcctgttt aggagagcct
      541 ggcggtcttc gattgtcacg tagtagcggg ggcaggactt gaacctgcga ccttcgggtt
      601 atgagcccga cgagctgcca ctgctccacc ccgcatcgaa gcccgccgat attaccatac
      661 ctgccaaaca gagtcaagcg acctgaccag aatcaatcaa gtagttcatt agtacatata
      721 tgtaatttac atcttgataa tattttgata ttgtagttta atcggcggac gacctccaaa
      781 gttcaatgcc gttgcaagaa acttttgtta agattagggt ataatgattt ttagatagcg
      841 ttcgttgcta ttatttgggc aacttcaaaa gaatgaaaaa tacaacactc ctggttattg
      901 aaggacgaca tgcagaaatc ccttcttttg ctcctgactt gcaaaagaag ggatttgatg
      961 ttgtttcatt ccaaaacggg agtcaagccg tctccaagct gaaacaaacc gaagcgagcc
     1021 tggtcgttat caatgctgca tccctgcgaa gcactgggat acgcatttgt ctttccgtcc
     1081 gcaagaaaga acccaagaca cccatcatct taattgtgga aaatgaaaaa gcagtggata
     1141 aggaactcgc cgattccgtg ttggtgctgc cattcactgt acaaaaactt gtcaatcgta
     1201 tcaaagctct aatgcctggc gacggaaaca acgtcataaa cgtaggtccg attcgtcttg
     1261 atgttgagca ccgacgcgtg aaaagccttg gaaaaaatac caggctcact ccccggctga
     1321 ttacgctact acaaatactg atggataaac atggtgaagt agtggagcgc gaatctctct
     1381 tcaaaaaagt ctgggagaca aattacaccg gtgacacacg cacgcttgat gtccatattt
     1441 cttggctgag acgcgccatc gagcttgacc ccgacgatcc aaaattcctc aaaacaatcc
     1501 gcggtgtagg atatcgttta gacgtttagc tgaaactgtt ccccctgcgc agtaacaaac
     1561 cggatgcaat gattgccccc ggtttttatt tttaattcat ggggaatttt tccgtttcgc
     1621 actggacgtc tggcctgggc gaaactcttg aacaatgtcc cgtcccaatc gatgtctact
     1681 tcccaataat cgagatcaag atcggattca attgagatga aatgatccaa ccggttcact
     1741 tttaccgagt tcggcgcgag gattgtaggg aaagcaaaat tgctatcatg ctcaagaaga
     1801 aaaggagctt gagtttcggt cagtcgtgca cgagtagtat gcaaggcatg gaacgtctca
     1861 tcacacgcaa taaatctgcg accatgcttt gctgccacaa atggcgtagt gccggaacca
     1921 caaaagaagt cagcgacgat attacccgga ttggacgaag ccaggataat acgttccatc
     1981 aaaccaacag gtttctgagt cggataaccg gtccgttcgt tgtgcaatcg ggcaaccaca
     2041 ggaaaatacc accaatcttc tggaacctta ccccgtttaa gatcaggtac cttcccaaaa
     2101 cctgcctttg gtgatgagtt gaaggttttg acagtattct tgttgtacgg ttcacgaacc
     2161 gcatcgacat taaatgtata atccgcgcct ttgacatagg ccaaaatcgt atcgtgttta
     2221 cgattgaacg cgcggcgaat tggcgatgga ccatgatatg cccagataat ttcgttgagt
     2281 agatgatctc gcccaaaaag ttcatccagt aatacacggg cataagaatc tgcatgccaa
     2341 tccaaatgaa gatatagtgt tccattcggt tcgagcaggc gatacattac cgcaagtctt
     2401 tggtatagga aatccagata ggaatcaaga tcgtcccaag cgtcgtgata tccatcgaca
     2461 agtttccact tattgggttt gcgcgagtct tcgccgcgac caattcgcgc aggaaattta
     2521 cgatttgtaa aaaatggtgg atctgcataa atcaaactaa ttttcccctc gaactctggc
     2581 aaaagcgcag acattaccgc taaattatca ccaagaatca atcggttttc aatatcaagt
     2641 agtgggtacc catagccatt tgggtagata atggagtctg ttgcaagctg cgctggcgcg
     2701 ggttgcgcaa tcccctttcc gtgccaggtc aattcgggca tatctaatag taaatttgaa
     2761 ccagggttcc aaaaccgcga tttgcgtgtc tgcccatttc atgctttggc agcgcccagc
     2821 ggtagagcca cttagcgtcc tcagtacgca tgttaatgca gccgtggctc atgggcacgc
     2881 caaagttctc atgccagtac gtaccatgaa atgcatgacc ctgagttgta aagaagcaag
     2941 tccacgggac gccagggagt tcgtaatcat ctgcatcagc aaaaagattg ccattgccca
     3001 tgtgcttgga gccgtccttc tccaggatcc gatactcacc ctgcggtgtc tttgtcgaaa
     3061 gaccgtccgc gccgcgggga ccgtttggca gaccggaagc gatggttgtt ttgaaaactt
     3121 ccctgtcatt ttcaaaagca acgaacttct gagtcgttaa gttaacttcg atgcgctttt
     3181 tttccagagg gatttctggg gtaatcgcct caatctcggc aagagggatc ggtcgtacat
     3241 gcagtgggtt aacgtgatac aaacctgcca aatcatcgtt aatctgatac cagggttcgc
     3301 cagtgggacc ttcctcaatc gcctgcaccc aatgcgtgga ctgataatac aaacgaaatc
     3361 cgagcttttg ccagccaaag gctttactcg ttcgccaggg ttcagtgaat gggacggtaa
     3421 tttcaaccaa acgtggtgtt ccgtcctcaa gagccgaaag cgttttattc agaagaatct
     3481 taacccgctg gagatgagat cgatgaacgt atccgcccca cacgcgatac caaatcggat
     3541 tgtgtgatgg ctcatccgct tccacttcgc catagattcg tagcaattca tcacgatacc
     3601 aggtcgaaac gatggaactt tcatcggtcg gtctgcggta gacactcacc gaattagtgg
     3661 ctatacgcac aaggttgctg tcatcaaaat cggaaagatt ttttaaggcg ggcgagaaac
     3721 ccaggcttcc caggctcaac ccacccagtt ttaggaaatc acgacgagat aaagatgcca
     3781 tagtgagaaa attgtaatcg aaaaccgagg tttctcaaat actctcacta agtttttatc
     3841 agaaaacaca actgccttcc gtgtacggaa ggcagttgat tattgattta attgtcaaag
     3901 attcgcttaa gattgaattg tttccggtac cataccctgt agcaggatct caagcgccat
     3961 agtatgcgca caacggtgat gagtcacata aaactcacag tcacaaccgt aaacgccgtt
     4021 atcgaaggtt acgtggtggt tattattatc tccatgaaaa gtaagatcaa acttgttgaa
     4081 gaggaagcgg tttctctctt cagcataacg tctggctttc tctctcttac ctatcattcc
     4141 atagtccatt ttatttatct cctttcaagc atatgaataa aaaaagcacg caaacagacg
     4201 cgtgctttcc ggttaatttc ttattcttat gtaggtatct gctcgcataa ggacatttac
     4261 ctcgatgcat tgagtatagt catattcgac aggcaagtca atgcccaaat gtgctatcgc
     4321 tctggatcgt acaggaatcg taaggtttca ctcaatccgc gggcgcggtc atggcgttcg
     4381 gctcatcgcc gttgtattgg gtgagaaccg gccaaatgga agccactagc cataccccca
     4441 gaatcgttcc tacccccccg ctaataatag caaatggaat gccaaaaagc tgcgcaacgg
     4501 caccagcttc tatttcgccc agctgcggtc cgcccatgaa aaagatctga tttatcccgg
     4561 tcatacgacc acgcatggaa tcgggggtat tgagctgccg aattgtgttg cggataatcg
     4621 tgctgaccga atcagccgca ccggccagcg ccagcgccag gaatatcagc gcataccacc
     4681 tcgaaaatcc aaaaaggatt gtcgccagcc cgaaaactac gaccgcgcca agcaaaagcg
     4741 gtccctggcg acggatatta ttgtattgtg agacaatcag cccgacgagg accgcgccaa
     4801 ttgactgggc ggctgccagc cagccatagg ccacttcccc cacatggatg acgtgttgtg
     4861 caaagtacgg gagtagtgta ttggccgagg aaaagaaggt ggcaataaaa tcgagaatca
     4921 tcgtcgagag aatgagtggc tgtcgacgaa tgaaccgaag cccatcacgg atatcaacta
     4981 acgccgggcg caatccagac tgtgatttgg attgttgctg cggtacattt cccagggcaa
     5041 tcaacgcaaa gatcaccgct aaaaacgtta cagcattgat aaaataagca aactcctgcc
     5101 caagatagcc gatcacgatt ccgctcatgg cggggccaac aaccgcaccc gtgttaaagg
     5161 caatggactg caacgagaac gcgctgggca atatatcgcg tgggatgaga tttggtacca
     5221 gcgcctgacg ggcaggcaag tcgaaggcca tggccgcggc ttgtatgccc gtcagtaagt
     5281 agatatgcca
//
LOCUS       DASTJP010000349         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1165484, whole genome shotgun sequence.
ACCESSION   DASTJP010000349 DASTJP010000000
VERSION     DASTJP010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165484"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFQ23_10420"
     CDS             <1..244
                     /locus_tag="VFQ23_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9907048.1"
                     /translation="TGQSDAEQARFLGFAIRSLIETIACHHIIQRRGYIQDQALLTKI
                     YEDSQVLAKKLQSFRKTVAPYQVREDQSPYGEDDLP"
     gene            281..1033
                     /locus_tag="VFQ23_10425"
     CDS             281..1033
                     /locus_tag="VFQ23_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET9907049.1"
                     /translation="MKIEIENLSLQYADGAESLRGISMGIRQNAVTVLFGPAGGGKST
                     LLRCLNRLNDLTEVKASAGRVLIDGENVLDPDTDVIALRRKVGMVFARPVVLPMSIRE
                     NITYGLELAGEKRRSKLDEAVERSLKLAAIWEEVKDRLDDPAIALSGGQQQRLCLARS
                     LALQPEIILLDEPTSGLDPISTGKVETALAELKKEFTIILVPHSVQQAARTADYAAFF
                     LQGELVEYSEGKTIFTAPKEKRTEDYVTGRFG"
     gene            1117..1521
                     /locus_tag="VFQ23_10430"
     CDS             1117..1521
                     /locus_tag="VFQ23_10430"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006637136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET9907050.1"
                     /translation="MKTVLFLCTGNSCRSQMAEAIVNARYPEWNAFSAGTKPAGYVHP
                     KAIQALEEIGIAHSGQSKQIDELHTKDFDLVVTVCDSAAEECPVWPGKAGKRVHHSFV
                     DPAKAEGTDEEQMQVFRQVRSEMLEVIPELLK"
     gene            1590..>2268
                     /locus_tag="VFQ23_10435"
     CDS             1590..>2268
                     /locus_tag="VFQ23_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907051.1"
                     /translation="MISPFDILFEPVRIGPVTAPNRFYQVPHCNGFGHRMPQGMAAMR
                     GMKAEGGWGVVCTEETEIHHSSDLSPYFEGRLWSDDDIPTLALMTEAVHKHGALAGIE
                     LSYNGIDAPNLYSRVPSLAPRSMGTVGGSGYDPVQTRRMDKEDIRNVRKWHRDAALRS
                     QRAGFDIIYCYAGHGMTAAIQFILKRYNDRTDDYGGSLENRIRFFRELIEETKEAVGD
                     TCAVAVRF"
BASE COUNT          599 a          539 c          607 g          523 t
ORIGIN      
        1 aacaggtcaa tcagatgcgg agcaggcaag atttttaggt ttcgccattc gatcacttat
       61 tgagaccata gcctgtcacc acatcattca acgcagaggt tatattcaag accaggctct
      121 ccttacaaaa atctacgaag actcccaagt cctcgccaaa aaattgcagt cctttcgaaa
      181 gacagtcgca ccatatcaag tccgagaaga ccaatcccct tatggagaag atgatctacc
      241 gtagtatatc cattgtccac ggtctatcgt ccatcgtcta atgaaaatcg aaattgaaaa
      301 tctctccctc caatacgccg atggcgctga gtctttgcgc ggcatcagca tgggaatccg
      361 ccagaatgca gtgaccgttc tcttcggtcc ggcaggaggc ggtaagtcca ccctgttgcg
      421 ctgtttgaac aggttaaatg atctcactga ggtcaaagcc agcgcagggc gtgtccttat
      481 cgacggtgaa aatgtgcttg accccgatac agatgtgatt gcattacgtc gcaaagttgg
      541 aatggtcttc gcaagacccg ttgttttacc gatgagcatt cgtgaaaaca tcacttacgg
      601 tttggaactt gcaggcgaga agcgcagatc caaactggat gaagcggtcg aacgcagcct
      661 gaaactggca gccatttggg aagaggtcaa ggatcgactt gatgatcctg caatagccct
      721 ctcaggcggg cagcaacaac gtttatgtct ggcgcgctcc cttgcattgc aacctgaaat
      781 cattctactg gatgaaccga catcaggtct cgacccaata tcaacaggca aagtggaaac
      841 tgcactggcc gaattaaaaa aagaatttac gatcattctt gtgcctcatt ccgtgcaaca
      901 agcagcacgt actgctgatt atgcggcgtt cttcctgcaa ggcgagcttg tcgaatacag
      961 cgaaggtaaa acgattttca ccgcacccaa agaaaaacgg actgaggact acgtcacggg
     1021 gaggtttggg tagtagttat atggtcgttg cgagcaggga gttagtccgg aagactacgc
     1081 gccaacgagc ggcgactctc ggtggcacaa agatatatga aaactgttct ctttctctgc
     1141 acaggcaact catgccgttc gcaaatggcg gaagccattg tcaatgcgcg ttacccggag
     1201 tggaatgcct tctctgctgg gacaaagccg gcgggctatg tgcatccaaa agcaattcag
     1261 gcgttggaag aaattgggat tgcacattca ggtcagtcaa aacagatcga tgaattgcat
     1321 accaaagatt ttgatcttgt tgtaacggtt tgtgattcgg ccgcggagga atgtcccgtg
     1381 tggcccggaa aggcagggaa acgcgtccac cacagttttg tggatcctgc aaaggcggag
     1441 ggaacagatg aagaacagat gcaggtcttt cgtcaggtac gaagcgaaat gctggaagtc
     1501 atcccggaac tgttgaaata aatccaaaaa caaatcattt gacgggctga gtctgggaat
     1561 ggtattcttg acgaaaaaag gataccccca tgatcagccc atttgacatt cttttcgaac
     1621 ctgttcgaat cggacctgtc acagctccga accgttttta tcaggtacca cactgtaacg
     1681 gattcggcca ccgcatgccg caggggatgg cggcaatgcg cggcatgaaa gcggagggag
     1741 gctggggtgt ggtctgcacg gaggaaacgg agatccatca ctcaagtgat ctgtctccat
     1801 acttcgaagg gcggttgtgg agcgatgacg atatcccgac gctggcgttg atgacagagg
     1861 ccgtgcacaa gcatggcgcg ctggcaggga tagaactgtc ttataacgga atcgacgcgc
     1921 cgaatttata ttcgcgcgta ccctcgcttg cgccacgttc gatgggaact gtgggtggtt
     1981 caggctatga ccctgtccag acccggcgca tggacaagga agacattcga aacgtacgca
     2041 agtggcatcg tgacgcggca ctgcgctccc agcgagctgg cttcgatatt atctattgtt
     2101 atgcgggtca tggaatgact gcagcaatcc aattcatttt gaagcgttac aatgatcgca
     2161 cggatgatta cggtggaagt ttggagaatc gcattcgttt ctttcgcgaa ttaattgagg
     2221 aaacaaagga agcagtgggt gacacctgcg cggtggctgt ccgttttg
//
LOCUS       DASTJP010000350         5748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_102289, whole genome shotgun sequence.
ACCESSION   DASTJP010000350 DASTJP010000000
VERSION     DASTJP010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102289"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..917)
                     /gene="pstA"
                     /locus_tag="VFQ23_10440"
     CDS             complement(54..917)
                     /gene="pstA"
                     /locus_tag="VFQ23_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9907052.1"
                     /translation="MKRPFTLNRHLTERLGFTFLTLMAIATVIPIVATVLYILIKGLP
                     AISLEFLTGFPREGMRAGGILPAIIGTIYLTLGTAIFSVPLGIAAAIYLSEYASDTPL
                     TRTIRIAIINLAGIPSVVYGLFGLGLFVLFLNFGTSILAASLTLSIMTLPVIISTAEE
                     SLRAVPQAFRTVSISLGATRWQTIRRIVLKEALPGILTGVILGLERAAGETAPILFTG
                     AAFFLPQLPGSPFDATMALPYHLFVISTQVPEMPIQIQYGTALVLLVFVLTMNLIATV
                     IRSRARAKRQW"
     gene            complement(1049..1945)
                     /gene="pstC"
                     /locus_tag="VFQ23_10445"
     CDS             complement(1049..1945)
                     /gene="pstC"
                     /locus_tag="VFQ23_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET9907053.1"
                     /translation="MEKSLNWGEFIITRLIRASGYSAILFVALIFFFLVREGVPTFGE
                     VELSSLTNIRWYPIEDRFGILPLITGSLIVTLGAALIAVPFGIGTAIYISEIAPRWAR
                     EVLKPLVELLAGLPSVVLGFLGILVLSPYLRVLLDLPTGLTAFTGSILLGGIAIPTVV
                     SVAEDALDAVPRAYREGAWALGATRWQTIWRVTLPAARSGVLTAIMLGVGRAIGETMA
                     VMMVTGNAPVLATALGSLFSPVRTMTATIAAEMGEVASGSTHYHVLFFIGMVLFLISL
                     GVNIAASSVVFRAKKRAERILS"
     gene            complement(1952..2833)
                     /locus_tag="VFQ23_10450"
     CDS             complement(1952..2833)
                     /locus_tag="VFQ23_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9907054.1"
                     /translation="MKRSILLFLLSTFLVSSCTLNSSSDSSSNSSALYIENKGSDTIV
                     NLALAWAERYQSEYPEVRISVTGGGSGTGIASLINGTVDIANASRKIKEEEIGEAESN
                     GVEPVEFIIARDAIAVIVNPENSVNQLTLKQISDIYSGKYTNWSELGGDDRPIVRLSR
                     ETNSGTHVYFLETVLRLGNSEDTTLFSMDTLLLPSSEGIIVEVRQNPNAIGYDGLGYV
                     PDDLKMIAIAEEEGGAYVLPSIPTVNDKTYPIARDLYMYTNGEPTGVVKDYLEWILSA
                     EAQEIVAELGFVPVGGE"
     gene            2865..3623
                     /gene="pstB"
                     /locus_tag="VFQ23_10455"
     CDS             2865..3623
                     /gene="pstB"
                     /locus_tag="VFQ23_10455"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET9907055.1"
                     /translation="MPESQSVFSVQNLDFYYGQFKALSNINIEIKPRKVTALIGPSGC
                     GKSTFLRCLNRMNDTIAGTRVDGKILLNGQDIYGSDMDVVELRQRVGMVFQKPNPFPQ
                     PIYDNVAFGPRVMGMNVNMDDVVETSLRAAALWDEVKNDLRSDAMGLSLGQQQRLCIA
                     RVVAVHPEVILMDESTSALDPIATLHVEELIAELKKDYTIVIVTHNMQQAARVSDYTG
                     LFWLGELVEFSPTQEMFTKPKQELTEAYITGRMG"
     gene            complement(3826..4524)
                     /gene="phoU"
                     /locus_tag="VFQ23_10460"
     CDS             complement(3826..4524)
                     /gene="phoU"
                     /locus_tag="VFQ23_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET9907056.1"
                     /translation="MLRKTFATEIQQVKDDVLVLGSMVEQAILGSVDALKKRDIKASQ
                     KIFENDLEINKQRFDIENKLMILIATQQPMAHDLRLLASIMEIISELERMGDYAKGIA
                     NINIRMGDTPLLKPLIDVPRMAQIGVDMLHQALTAFVNEDVEAAKAIPVHDDEVDALY
                     NQVYRELMTFVMQDPKNIERANWLLWVAHNLERVADRVTNICERTIFIVTGEMQEIKS
                     TDDEFWHQQKQNNS"
     gene            complement(4635..5084)
                     /locus_tag="VFQ23_10465"
     CDS             complement(4635..5084)
                     /locus_tag="VFQ23_10465"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET9907057.1"
                     /translation="MQKPRVLFLCTGNSARSQMAEAFLRAYANDYFEVHSAGIEPKGY
                     ILPEVRSVMKEHGLDLSGQRSKDIREYLGQTHFAHVITVCADAEENCPTVFLNMGIHE
                     HWGFDDPAKFQGNEAEKLEFTRNVRDQMDQHIQHWLVSQNIPLTLLK"
     gene            complement(5102..>5748)
                     /locus_tag="VFQ23_10470"
     CDS             complement(5102..>5748)
                     /locus_tag="VFQ23_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET9907058.1"
                     /translation="GKSTLLRSFNRMNELYAGTSVKGKITFNGEDIYAREVDPVEVRY
                     RIGMVFQKPNPFPKSIYENIAWTARIHGFKGNMDELVENSLRQAAIWDEVKDKLHQSG
                     LSLSGGQQQRLCIARAISIKPEVILMDEPASALDPIATLKIEELMQEIKKNYTIVIVT
                     HNMQQAARVSDYTAMMMLTTESSSGTVIEFDDTKRIFTNPKDKRTEDYVTGRFG"
BASE COUNT         1478 a         1385 c         1419 g         1466 t
ORIGIN      
        1 atggcatgca gattctcaac aatggtctat cgtcggtcgt ccattgtcca aaactaccat
       61 tgacgttttg ccctggctct cgaacgaatg acagttgcga tgagattcat tgtcaacaca
      121 aacactaaca agacgagcgc cgttccgtat tggatttgaa tcggcatttc aggaacttgt
      181 gtggagatca caaagagatg atagggaagc gccattgtgg cgtcgaacgg tgagccggga
      241 agctgtggca ggaagaatgc ggcgcccgtg aatagaattg gcgcggtctc gcccgcggcg
      301 cgttccagtc cgaggatcac gccggtcaga atgccaggca aagcttcctt caacacaatg
      361 cggcgaatgg tctgccagcg cgtggcaccc agtgagatac tcacggtccg aaatgcctgc
      421 ggaacggcgc gcaatgattc ctcagccgta ctgatgatga caggcagggt catgatcgac
      481 agggtgagcg atgctgccag gatggaggtg ccgaagttga gaaagagaac aaagagaccc
      541 agtccaaata atccgtacac cacagatgga atcccagcaa ggttgatgat cgcaattcga
      601 attgttcgtg tgagaggtgt atctgacgca tattcggaga gataaattgc cgcagcgatt
      661 ccaagaggga cagagaatat ggcagttccc aatgtgagat agattgtgcc aataatggca
      721 ggcagaattc cacccgcacg catgccttcg cgcggaaacc ctgttagaaa ttcaagcgag
      781 attgcaggca gaccttttat cagaatatac aaaactgttg caacgatagg gataactgtg
      841 gcaattgcca tcaatgtaag gaaggtgaag cccagccttt cggtgaggtg gcgattaaga
      901 gtgaagggtc gtttcatctt ttattcatac tcggtggtcg ggtagccttg cgcaggttga
      961 gaccatccat ttcaagtgat ctttgtttga atgcaatgcg ttggttcggt acatcctgtg
     1021 ggatgactcg accaacggat gattctcatt atgacaaaat tcgctctgcg cgcttcttcg
     1081 ctcgaaaaac aacagacgaa gccgcgatat taacgccaag cgagatcaga aataaaacca
     1141 tgccaataaa aaacaacaca tgataatgcg tactaccact cgcaacctct cccatctccg
     1201 ctgcgatggt agcagtcatt gtacgaacgg gcgaaaataa acttcccagt gccgttgcaa
     1261 gaacgggtgc attcccagta accatcatga ctgccatggt ttcaccgatg gctcgcccaa
     1321 ccccaagcat gattgcagtg agcacacctg agcgtgcggc tggaagtgtc actcgccaga
     1381 tcgtttgcca gcgtgtggcg cccaaggccc aggcaccttc gcgataggca cgcggcacag
     1441 catccagcgc atcctctgcg acagaaacca cggtcggtat tgcgatcccg ccgaggagaa
     1501 tggaaccggt aaaagcagtc agaccggttg ggaggtccag aaggacacgc aggtacgggg
     1561 aaaggacaag gatgccaaga aatcccaata ctacagaggg aagcccggcg agcaattcca
     1621 caagaggctt gaggacttcg cgcgcccagc gtggtgcaat ctcagaaata taaattgccg
     1681 ttccaattcc gaacggaacc gcaatcaatg ctgcacctaa cgtaacgata agggagcccg
     1741 tgatgagagg caggatccca aaccgatctt cgattgggta ccagcgaata ttcgtgaggg
     1801 aggagagttc cacttccccg aacgtgggca caccttcgcg aacgagaaag aaaaagataa
     1861 gcgcaacgaa gaggatggca gaatacccag aagctcgaat gagccgcgtg ataatgaatt
     1921 caccccaatt taaagatttt tccatttcac ctcactcgcc gccgacgggc acaaaaccaa
     1981 gttcagcaac gatttcctgt gcttctgcag aaaggatcca ttcgagatag tccttgacca
     2041 cgccggttgg ttcaccattg gtgtacatgt atagatctcg cgcaatcgga tacgtcttat
     2101 cgttcacggt cggtatggat gggaggacat atgccccacc ttcttcctct gcaatcgcga
     2161 tcatctttaa atcatcaggt acatagccaa gcccgtcata gccaatggca tttgggtttt
     2221 gtctgacttc tacgatgatt ccctcggagg agggcagcag caatgtgtcc atggagaaca
     2281 gtgttgtatc ttcgctattc ccaaggcgca ggacggtttc aagaaagtaa acatgggtgc
     2341 ccgaattggt ctcgcgtgaa agacgcacga tcggtcgatc gtcgcctccg agttctgacc
     2401 agtttgtgta cttgccacta taaatatcag atatctgctt cagggttaat tgattgaccg
     2461 agttttctgg attcactatg actgcgatgg cgtcacgtgc gatgataaat tcgactggtt
     2521 cgactccatt tgactccgct tcaccgattt cctcctcttt gatttttctc gacgcattgg
     2581 cgatatctac cgtgccattg atgagtgatg caattcccgt cccggacccg ccacctgtca
     2641 ccgatatacg gacctccgga tactcacttt ggtagcgttc tgcccacgcc agagcgagat
     2701 ttacgattgt gtcggaaccc ttgttttcaa tgtatagtgc agaagaattt gaggatgaat
     2761 cagaggatga attaagtgta caggatgaaa caaggaaagt tgaaagaaga aagagaagta
     2821 tcgagcgttt cattactgtg aattatatct gataaaatct actcatgccc gaaagccaat
     2881 ctgtcttctc cgtccaaaac ctggattttt attacggtca attcaaagcg ctctccaaca
     2941 tcaacatcga aatcaagccg cgtaaagtaa ccgcgttgat cggaccctca ggctgcggta
     3001 aatccacgtt tttgcgttgt ctaaatcgca tgaacgatac catcgccggg acgcgcgtgg
     3061 atgggaaaat tttgcttaac gggcaggata tctacggctc cgacatggac gttgtggaac
     3121 ttcgtcagcg tgtgggcatg gtgttccaaa aacccaatcc ttttccgcag cctatttatg
     3181 acaatgtcgc gttcggtccc cgggtgatgg gcatgaatgt gaacatggat gatgtagtgg
     3241 agacatctct gcgtgcggca gcattgtggg atgaagtaaa aaatgactta agatcagatg
     3301 cgatgggact ttcattgggt cagcaacaaa gattatgtat tgctcgcgtt gttgctgttc
     3361 atcctgaagt gattctgatg gatgaatcta cttcggcact ggacccgatt gctaccttac
     3421 atgtcgaaga actgattgca gagttgaaaa aggattatac gattgtcatc gtaactcata
     3481 acatgcagca agcggcgcgt gtgtctgatt acacgggtct gttctggctg ggtgagctgg
     3541 tggagttttc cccaacccag gaaatgttta cgaaaccaaa gcaggaactg acggaggcgt
     3601 atattacggg caggatgggg taaaggatcg tagaacggcg ggtgggccga gttctgttaa
     3661 tagtctattc ttcaaaccag cgggaaacaa acttttaacc gcctgttaac tggatcaaac
     3721 aggctaggca ggatcacttc ttcattccag gtcagttttg gcacacccat gaaacggttg
     3781 ttaaaacaaa agtgcggata tctcagatat ccgcactttt acaaattatg aattattttg
     3841 tttttgttgg tgccagaact catcatcggt tgatttgatt tcttgcatct cacctgtaac
     3901 gataaaaata gtacgctcac aaatattcgt cacgcggtct gcgacccgtt ccaggttatg
     3961 tgccacccac aacaaccagt ttgcacgctc gatgtttttg gggtcttgca tgacgaaggt
     4021 catcaattca cggtacacct gattgtaaag cgcatccact tcatcatcat gtacggggat
     4081 ggcttttgcc gcctccacat cttcattcac aaatgcagtc agggcttgat gcagcatatc
     4141 cacaccgatt tgtgccatgc gcggcacatc gatgagaggt ttgagtagcg gcgtatcccc
     4201 catccgaatg ttgatgttgg cgattccttt cgcataatca cccatgcgtt ccaactctga
     4261 aatgatctcc atgatggaag ccagcaatcg caggtcatgc gccatgggct gttgcgtagc
     4321 aatcagaatc atcaacttat tttcgatatc aaagcgctgc ttgttgattt ccagatcgtt
     4381 ttcaaagatc ttttgcgatg ctttaatatc acgcttctta agcgcatcca ccgaaccaag
     4441 gatggcttgt tccaccatgg aaccaagcac aagcacatca tctttgacct gctggatttc
     4501 agtagcaaag gttttacgaa gcatagtacc ctcgtctctc tgtggtcatt ctattatact
     4561 gcccttctgt gagttgttta tcttatcaga caacgcgaaa agtttatcac ctgtcaagta
     4621 agacagcgaa gtagttattt taggagcgta agcggaatat tctgagagac cagccagtgc
     4681 tggatgtgtt ggtccatttg atcacgcacg ttccgggtga attcaagctt ttctgcttcg
     4741 ttaccttgaa attttgccgg gtcatcaaat ccccaatgct catgaattcc catgttaagg
     4801 aatactgtgg ggcaattttc ttcggcatct gcacacacgg ttattacatg tgcaaaatgt
     4861 gtttgcccaa gatattctct tatatctttt gatctttgcc cacttagatc cagtccgtgt
     4921 tctttcatca cactccgaac ctctggcagg atgtagcctt ttggctcgat accagcgcta
     4981 tgcacttcaa aataatcatt ggcataagca cgcaaaaagg cctccgccat ttggctgcgt
     5041 gcagaattac cggtacaaag gaataaaact ctgggctttt gcatatcaat atcctcgtat
     5101 cttatccaaa ccgtcctgtg acgtaatctt ccgtacgctt gtctttgggg ttggtgaata
     5161 ttcttttcgt atcatcgaac tcgatcactg ttccagaact gctttcggtc gtcaacatca
     5221 tcatggcagt gtagtcagaa acacgtgccg cctgctgcat gttatgggtc acaatgacga
     5281 tggtgtaatt tttcttgatt tcctgcatca actcttcaat cttgagcgtg gcaatcgggt
     5341 ccagagcgga ggcgggttcg tccatcagaa tgacttcggg tttgatggaa atcgcccgcg
     5401 cgatgcacaa gcgttgctgc tgaccacccg agagcgataa cccggattgg tgcagtttgt
     5461 ccttgacttc atcccagatc gcggcttgcc gcagtgagtt ttccaccaat tcatccatgt
     5521 tgcctttgaa gccgtggatg cgggccgtcc aggcgatgtt ttcgtagatg ctctttggga
     5581 acggattggg cttttgaaag accatgccga tccggtaacg aacctccacc gggtccacct
     5641 cccgggcgta gatatcctcg ccgttaaaag tgattttccc tttgacggaa gttccggcat
     5701 atagctcgtt catacggtta aaagaacgca gcagagttga tttgccgc
//
LOCUS       DASTJP010000351         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_5085,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000351 DASTJP010000000
VERSION     DASTJP010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5085"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1731..>2852)
                     /locus_tag="VFQ23_10475"
     CDS             complement(1731..>2852)
                     /locus_tag="VFQ23_10475"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9907059.1"
                     /translation="FITFESFASLVAENQLDFHPIRGNAQTVVTNGGANLFGLIRSFG
                     SLAEGYARDLSAPYLGTTDLIINQLPAGLYGNDLAEKYGVPMVMASVIPLARTKMFPL
                     MGFPRLRMPGYNKATYYLGEQIAWQMFRSVINRWRKETLCLPPLPVKGYFGQVGTRQI
                     PIVNGFSQHVVPRPRDWNEHIHVTGYWFPENKHWQPSSDLSEFIEAGKPPVFIGFGSM
                     PIKDPQRTTKIILEALKNSEQRGILHMGWGGIGNQSLPDYVFKIDYAPYDWLFPRMAM
                     VIHHGGSGTTAFGLRSGIPSCVVPFVFDQFYWGERIAQLGVGPKPIPFKELNVKHLTE
                     AIRSGVGNLQIKRNAFALGQRIQAEKGIENALNVFEKMM"
BASE COUNT          764 a          717 c          639 g          732 t
ORIGIN      
        1 atttttgttc tgaaacttgc ggacagcgct tcgtcctcgg cacgtacctt ttcaacatcc
       61 aacatgcctg ttggcggatt aaggagacgc cgtatctgaa aaattgctct gatcaggcgg
      121 aattgccagc ttggcataac gatcattctc ctttcattga atatccttag gccgcccaac
      181 ggctcacgtt atgccccttc aggggtaccg gcgggcgaga cgagacaacg ctattttgac
      241 ggaaccagct tcaagccacg caaagtgccc gaaaacgcgc acgccgcgaa gcggcagtcc
      301 caccaccatg gtttcggtgc ccttccgagg gtcgggtgca ggctatgttg ggtggcttca
      361 tggatcgcaa gacttctcac ctgaaaaaga caccaccgcg aatatgaaat gattttacac
      421 acattttgac tttggtaagg ctctaaaatc tgtttgacca aaactattga cccgactgga
      481 ttgcgaagaa caccagaaag agaagcgcaa tcaaatagac tcctatgctc agcaacatca
      541 aaaggcttcc agcaattgaa tatacccatc gtctgagggt agtaacgttc ctgatcgact
      601 taagttttgc acctgcatag cccaaaaccg cgagcaccat accaattaga agagggaaga
      661 gaaggaagta gagccaccaa tcgggatcgt tgacaattct gtcgtaagca agccagcgaa
      721 aagggtgagc tccagagaat aaaacaaata ggaaaccaac caagaaagga agtatcacca
      781 agcctgctgg agccaagaac atccatttat atctagaagc gctgggcaga attggagtca
      841 aagcttgtga tttcctcatc gtgtgatttc gtgtctctct gttgactgtt tagagccacc
      901 caccagctcg cgttatgccc cttcaggggt actggtgggt ggggcgggac cacgccattt
      961 tgacggaacc aagtccaagg ccacacaata cgcttgaaaa cacgcacgcc gcgaagcggc
     1021 agtaccatca ccacggtttc ggttctcttc cgagggtcgg gtgcacgttg tgttgggcgg
     1081 ctttttattc atcaattgaa ttttactcgg aacaattcca tctttgtcca cacgaataag
     1141 caaacatact tatcttgttt gtcgaaacct ttcccttaac attggaaatc cctgccccgt
     1201 ccagaatccc ggagcgacat gcccgcttga catacctccg tgattatatc gttcgacaaa
     1261 aactgaatgt gactccttaa ttgacgctcc agttaattgt tcatacgttt gctcaaggag
     1321 aatgttgaac tcttcttctg tttccgggta ggcaagatct cccaatacgc ctttcatctc
     1381 tcgccaaaga tatggatctc cgcgtagtcc ccagcgagac ggtccctcct ggaaaatctg
     1441 cgacaatgtc ttttgttttg acataccagt tagatacctt ttgctgtgtt ttatttcaat
     1501 tttagctgca acgcaaggac catctttatt gtggatgatc ttgatttgtc ttttgccatt
     1561 tacttttcac ccatattttc gaagccgccc aacggctgcg ttaccggcgg ccggggagaa
     1621 aaaagctcgg aagcggaaaa ctgccatact tcagaacaat ctcaaaaaaa cgcgccgcgt
     1681 acccatctgt ccactgcaga ctgtgtcagg cgcatgcact gaacgccagt ttacatcatt
     1741 ttttcaaata cgttcagcgc attctctatc cccttttctg cttgaatcct ctgcccaagc
     1801 gcaaaggcat ttcgttttat ttgcagattg ccaaccccag agcgaatggc ttccgtcaaa
     1861 tgtttgacat tcaattcctt aaatggaata ggtttgggac ctactcccaa ttgggcaata
     1921 cgttctcccc aatagaactg gtcgaataca aatggcacta cgcaggatgg tatgcctgaa
     1981 cgaagaccaa aggcagtggt accggagccc ccatgatgga tgaccattgc cattcgtgga
     2041 aataaccaat catagggagc ataatcaatc ttgaacacgt agtcaggtaa cgattgattt
     2101 ccaatgccgc cccaacccat atgcaatatt ccacgttgtt cgctgttttt cagggcttct
     2161 aaaatgatct tcgtagttcg ttgcgggtct ttgatcggca tgcttccgaa accgatgaac
     2221 acaggcggtt taccggcttc aataaattct gataaatcac tggatggttg ccagtgctta
     2281 ttttctggaa accaatagcc tgtgacgtgg atatgttcat tccaatcccg tggacgtgga
     2341 acgacatgct gactaaaccc attcacaata gggatttggc gtgttccaac ttgaccaaag
     2401 taacctttca caggtaatgg aggcaggcaa agagtctctt tccgccaacg atttatgaca
     2461 gagcggaaca tttgccatgc tatttgttcg cccaaatagt atgtggcttt gttgtaacca
     2521 ggcatacgga gtctgggaaa tcccatcagt gggaacatct tggttcgagc caatggaatt
     2581 acagacgcca ttaccatagg cacaccatat ttttcagcca aatcattacc atatagtcca
     2641 gccggtaatt gattgatgat caagtctgtt gtacccaaat agggggcaga tagatcgcgg
     2701 gcataacctt ccgccagaga gccgaacgat cgaatcagcc caaacagatt tgcgcctccg
     2761 ttggtgacca cggtttgggc gtttcctcga atcgggtgaa aatctaattg gttctccgca
     2821 accagagaag caaaactctc gaaagtaatg aa
//
LOCUS       DASTJP010000352         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1163836, whole genome shotgun sequence.
ACCESSION   DASTJP010000352 DASTJP010000000
VERSION     DASTJP010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163836"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFQ23_10480"
     CDS             complement(<1..401)
                     /locus_tag="VFQ23_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907060.1"
                     /translation="MSYEIRRATAADKPEWLRMRQGLWPDAPIEYLDYDLDEILGDPD
                     QAVFIAAQADGKPVAFIETGLRSHVEGCETSPVGYIEAWYVDPHVRGQKLGREMMRTA
                     EQWAREKGMSEMASDTWLENEVSIAAHLKLG"
     gene            complement(398..784)
                     /locus_tag="VFQ23_10485"
     CDS             complement(398..784)
                     /locus_tag="VFQ23_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HET9907061.1"
                     /translation="MIEKLKSQARTLKNEVYILYLAAKDARTPWYAKALIFFVVAHTF
                     SPIDLIPDFIPVLGYIDDLIITPGGIWLAVRLIPPEVVAEARAAATTQGIDRYIAIVG
                     AGIILLVWILAIIWVAIIILRLVEKT"
     gene            846..1355
                     /locus_tag="VFQ23_10490"
     CDS             846..1355
                     /locus_tag="VFQ23_10490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907062.1"
                     /translation="MAAEKIIFTVRRAEPDDYKALHQIFASPQAVRGTLQLPFPTVEL
                     FRKRLTEPPEGTFNLVVCVEDEVIGQLGLHTFPNHPRRRHVGQIGMAVRDDWQGKGAG
                     TALMQAMIELADRWLNLSRLELEVYTDNEPAIRLYKKFDFVIEGTHTDFAYRDGTYVD
                     AHFMARLRK"
     gene            complement(1358..2326)
                     /locus_tag="VFQ23_10495"
     CDS             complement(1358..2326)
                     /locus_tag="VFQ23_10495"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET9907063.1"
                     /translation="MTNFPADEVRFFSGSSNPALAGNIAKELGVPLDSTSVTRFSNDN
                     MYLQLGASVRNRRVYIVQSLSQPVNDHLMELLMMLDIARSAAASEVHAIIPYYSFGRS
                     DKKDAARISITARLVADLIQTAGASHVMSMVFHSPQVHGFFGLPTDPLSTRPVFGKHM
                     AERDLTGFIVVAPDMGQAKSAARFAKSLALPIAAGNKERVSDNEVRISGLVGNQVRGH
                     ERALVYDDEIATGSSVLELSRMLVNEGIKEIIVMCTHGVFTRGGLEKLGDMPHISEII
                     TTDTVYIPPEKQHPKLKILSVASVFADAIRHNFNRESIGNLFVYGE"
     gene            2475..>4204
                     /locus_tag="VFQ23_10500"
     CDS             2475..>4204
                     /locus_tag="VFQ23_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9907064.1"
                     /translation="MDLLERDLQLKALRSALNEAGHEGRVAFVYGEAGIGKTALVEHF
                     IKENEKSWRILHGACDSFFTPRPLGPLYDIALQTERDLSAFLESESNRGRLFSACLSE
                     LQTQKTILVIEDIHWADEATLDLLKYLGRRIRQTKALLILTYRDDEIGTDHPLRLLLG
                     DLASTHALHRISVSLLSKNAVQKLANHKNVDSLQLHRLTNGNPFFITEVLAAESGIPE
                     TVRDAVLARAARLSPSARALLEAAAVIGSRLEAWLLSEIVGADIVSVEECIARGLLQL
                     QGDYYTFRHELSRQTILESISSQRKITLHRKTLNALQESSVTRESMTRLAHHAEAIND
                     PDAVLEFAPLAAWWASTTSSHREAVALYELALRFAKSLPPANHAQMLEAYMVELEFMN
                     RFSDEIEACQSAIALWHSIGNRLREGKNLATLAGLCMNVGRKSETEKSIERAITILED
                     LSPSAELAQAYTEQCYIKMMYRDCEEAITWGEKATTLAERFDDFDALARAYDYMGCAA
                     LIIDYDRGLKLLERSLVIAREKKLPFAVGGTLGNLGGMLLEVRQVTLANQYLAESIDY
                     ATQQDNDYHL"
BASE COUNT         1034 a         1063 c         1063 g         1044 t
ORIGIN      
        1 tatcccagtt tgagatgcgc ggcgatactc acttcatttt caagccatgt gtcagacgcc
       61 atctcagaca tccccttttc acgggcccat tgttccgcgg ttcgcatcat ttcccggcca
      121 agtttttgac ctcgcacatg cgggtccaca taccaggctt caatgtagcc taccggactg
      181 gtctcacagc cttccacgtg acttcgtaat ccggtctcga taaaagcgac cggttttcca
      241 tccgcttgtg cggcgatgaa cacggcttga tcaggatccc ccagaatttc atcaaggtca
      301 taatccaaat actcaattgg cgcgtcgggc cataatccct gacgcattcg tagccattcg
      361 ggcttatctg ccgcagtagc acggcggatc tcataactca tgttttctcc accaaacgaa
      421 ggatgatgat tgcgacccag attatagcca atatccagac cagcaggatg attcccgcgc
      481 cgactatggc gatataccga tctatacctt gcgtggttgc ggctgcccgt gcttctgcta
      541 ctacctcagg aggaattaat cgcactgcca gccagattcc tcccggcgtg atgatgagat
      601 cgtcgatgta cccaaggaca ggaatgaaat cagggatgag atcaatcggc gaaaaggtat
      661 gcgccaccac gaaaaatatc agtgcttttg cataccaggg tgtccgtgca tccttcgccg
      721 ccaggtagag gatatatact tcgttcttaa gagtacgtgc ctgtgatttt agtttctcta
      781 tcataaagat tgttttcaaa attgatttgt tttatactga cgacaaagaa gttgaggtaa
      841 ttaacatggc agctgaaaaa ataattttca cggttcgtcg cgcagaaccc gatgactaca
      901 aagcgctcca tcaaatattt gcaagtccac aggcagtgcg gggaactctg cagctgccat
      961 ttccaactgt tgaactattt cgtaagcgcc tgaccgaacc gccagaagga acattcaacc
     1021 tggtggtttg tgtggaagat gaagtgattg gtcagttggg actacataca tttcccaacc
     1081 atccccgccg ccgtcacgta ggacaaattg gtatggctgt gagagatgac tggcagggga
     1141 agggcgcagg caccgcgctg atgcaggcaa tgatcgagct ggcggaccgt tggctaaatc
     1201 tgtcgcggct cgaattggaa gtttatacag acaacgaacc cgccattcga ctctataaaa
     1261 agtttgattt tgttatcgaa ggtacgcaca cagactttgc ctatcgtgac ggaacgtatg
     1321 tggatgcgca cttcatggcg cggttgagga agtaaatcta ctcgccgtag acaaacaaat
     1381 taccgatcga ctcacgattg aaattgtgtc ggatggcatc cgcgaagacc gaagcaaccg
     1441 aaagaatttt cagttttgga tgctgtttct caggcggaat gtaaaccgta tccgttgtta
     1501 tgatctcgct gatgtgcggc atgtctccca gcttttccaa accgcctcgc gtgaaaaccc
     1561 catgcgtgca catgacgatg atctctttga tgccctcgtt gaccagcatg cggcttaact
     1621 ccagaacgga gctgcctgtt gcaatctcat catcgtagac gagcgcgcgt tcatgacctc
     1681 gcacctggtt gccgaccaag ccactgattc ttacttcgtt atctgaaaca cgttccttat
     1741 tccccgcggc gatgggcaaa gccaggctct ttgcaaagcg cgcggcagat tttgcctgtc
     1801 ccatatctgg ggcaaccacg atgaaaccgg tcaggtcacg ttctgccatg tgttttccaa
     1861 aaaccggacg agtgctgaga ggatcggtcg gaagaccaaa gaagccatgc acctgtgggg
     1921 agtggaacac catgctcatc acatgcgagg ctccggcagt ttggatcaga tctgcgacca
     1981 atcgagccgt aatggaaata cgtgcggcat cttttttatc tgagcgcccg aatgagtaat
     2041 aggggataat ggcgtgcact tcagatgcgg ccgcactgcg ggcaatatcc agcatcatca
     2101 gtagttccat aagatgatca ttcaccggtt gcgaaagcga ctgtacgata tacacgcgtc
     2161 ggttacgcac gctggcgcca agttgaagat acatattgtc attgctgaag cgagtgacgc
     2221 tggtgctatc caacggcaca cccagctctt tggctatgtt gcctgccaat gccgggtttg
     2281 aactgccgct gaaaaatcgg acctcatctg ctggaaagtt tgtcatcctg gctcccgtgg
     2341 caaaatcatt aggattttat aaccccatct tacactgact tctcttgaat tgaaagcaca
     2401 tccgatgcac aggatcgccg gtttaactat gtcaggccca gagggctgtg ctatactgaa
     2461 tcgagggatt tttcatggac cttctggaac gcgatttgca attgaaagct ttgcgctccg
     2521 cgctgaatga ggcgggtcac gaaggacgtg tggcattcgt ctatggtgag gcgggaatcg
     2581 gcaagactgc attggttgag catttcatca aagaaaatga aaaatcgtgg cggatactgc
     2641 atggagcctg tgactcgttt tttacacccc gcccgttagg tccgctgtat gatatcgctc
     2701 ttcagacaga gagagatttg tctgcgtttc ttgaatcaga atcgaaccgc ggaagacttt
     2761 tttctgcctg tctgagcgaa ctgcaaaccc agaagacaat actcgtcatc gaagatattc
     2821 actgggccga tgaagccacg ctcgacctgc tcaaatacct ggggcgtaga attcgccaga
     2881 caaaagcatt gctgatccta acctatcgcg atgatgaaat cggcactgac catcctttgc
     2941 ggctcctgct gggagacctc gcctccacac atgcgcttca tcgtatttct gtttccctgc
     3001 tctccaaaaa tgctgtccaa aagctggcaa atcataaaaa tgttgactcg ctgcaacttc
     3061 atcgcctgac gaacggcaat ccgtttttca tcacggaagt gctggctgct gaaagtggaa
     3121 ttcctgaaac ggtgcgcgat gcagtgctgg cgcgtgccgc acggctctcg ccttcggcgc
     3181 gcgctctctt ggaagccgcc gccgtgatcg ggtcacgttt ggaagcgtgg cttttgtcag
     3241 aaatcgttgg tgcagatatc gtatctgtag aagagtgtat tgcaagagga ctgcttcaac
     3301 tgcaaggtga ctattacaca tttcgtcacg agctttcgcg gcagacgatt cttgaatcca
     3361 tttcctcgca aagaaaaatc acattacatc gcaagacctt gaacgccctc caggaatcgt
     3421 ctgtcacgcg ggaaagtatg acgcggctcg ctcatcatgc tgaagcgatc aatgatcctg
     3481 atgcggtcct tgaatttgcc ccgctcgcgg cctggtgggc ttcgaccacc agttctcatc
     3541 gcgaggcggt cgcactgtat gaactagcct tacgctttgc caaatctctc ccgccggcca
     3601 accatgcgca gatgctggaa gcgtatatgg ttgagttgga gtttatgaac agattctctg
     3661 atgaaatcga ggcttgccag agcgcgatcg cgctttggca ttcgatcggt aaccgattgc
     3721 gggagggcaa gaatttggca acgttggcgg gactctgtat gaatgtcggt cggaagtcgg
     3781 aaactgaaaa aagtattgaa cgcgccatca ctatccttga agacctttcc ccaagcgccg
     3841 aattggcaca ggcctataca gaacagtgtt acatcaagat gatgtatcga gattgtgagg
     3901 aggcaattac ctggggagag aaagccacta cacttgcgga gcgattcgat gatttcgatg
     3961 ccctggccag agcatacgat tacatgggtt gcgcggcttt aatcattgat tatgatcgtg
     4021 gcctgaaatt acttgaacga agtctcgtta ttgcacgcga aaaaaaactt ccgttcgcgg
     4081 taggcggtac gctgggaaat ttaggcggaa tgttattgga ggtgcgccag gtcacccttg
     4141 ccaatcaata cctggcagaa agtatagact acgctactca gcaggataat gattatcacc
     4201 tgtt
//
LOCUS       DASTJP010000353        14230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_322125, whole genome shotgun sequence.
ACCESSION   DASTJP010000353 DASTJP010000000
VERSION     DASTJP010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14230
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322125"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..877)
                     /locus_tag="VFQ23_10505"
     CDS             complement(206..877)
                     /locus_tag="VFQ23_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HET9907065.1"
                     /translation="MEIKVKPTHEPLGDKGIYNAFFRWALLAAEEVAGKQGLTIVLRE
                     NGLERFIDNYPSEEMNASAGALQKDYTALCTGLLTFFGRAGRGGLIRIGKLSARMFIN
                     NYMDSMGMSAMVAAARLLPAGMKQKGGLESNLNIFKKLYKDVGIDWHAHIEDRGEKFA
                     YIVEDCTVCIDREADAAICTVFTGNISEATLWLMEKEYDVQEVECRAMGAKACVWEIS
                     KKPKA"
     gene            complement(1091..1657)
                     /locus_tag="VFQ23_10510"
     CDS             complement(1091..1657)
                     /locus_tag="VFQ23_10510"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9907066.1"
                     /translation="MRNETFDRRIQRTRKLLQEALLELILEKEFEAVTVQDVIQRANV
                     GRSTFYSHFKDTEDLFLSGFENLWSLFDQHLAAQAAQKASVWELSLIVFEHAQAYGGI
                     YRALVGRPGEKLMSAHMHRYLAALMRDSLKVQWTKDRQIPLEVVVYHLVSSLVSLLIW
                     WVNHDMSDPPQLMNEMYQLLTRPAIEKL"
     gene            1811..3406
                     /locus_tag="VFQ23_10515"
     CDS             1811..3406
                     /locus_tag="VFQ23_10515"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET9907067.1"
                     /translation="MENKNTAVVKSFLDALFSGGTAEASEILADNLKVDNFFPHQSSK
                     KAFLRAFQQVLKAMEGVQVQLNALQGTENMVTAGFQVSGIHAQPLDFAFMKLPVVQPT
                     HKTVAWPALHWDFTISNGKIVNIRNTASLQKGMPGILKSFGVKMPAAHKFGSVLKALA
                     PAGKLKTNPKRFLRFLPLIPLTVILTTIFFVPSPINAAAWTPQPVPQAKGPLTTNYEL
                     TEKGELLGASQLQHAEDLAFDMDGRIYAGSDDGNIYRLTLDPAGQVSQFEAFATTGGF
                     PLGLQFDGSGQLIVAVKDIGLMSISKDGTAALLTDQVEGTPITYANAIAINADGLIYF
                     TDSSTKFDRGWPYDVLEARPHGRLLSYDPETGESSLIKDGLYFANGIFLAPDESYLLI
                     NESSRARISRYWLTGNQAGELDTFAENLPLLPDNISVDENGNYLLGGMRRLPMMDKLQ
                     PNDWLKNQIAKLPLPMLSKFPSLKMNRYGMILILDQNGKIKESLQDPTGKIFAISSAR
                     AYDGYIYISTLLGSDIARYPYHR"
     gene            complement(3624..4409)
                     /locus_tag="VFQ23_10520"
     CDS             complement(3624..4409)
                     /locus_tag="VFQ23_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907068.1"
                     /translation="MNQIPDEKQIEELLESIAPHTTSRLETKLADAPWSEGVVNRRRS
                     LQAISLAVLLVASVFAASPQGHAFAQDVLQFFTRTQSDSYYEPVSNLTFEETTPFHQQ
                     CGIPIRPTCSVEEIRSMVDFEVKELGTLPGDLYYVGATGGPDWVDLHYEYPNRLAGGI
                     GILVEATGRPAPGALHIVPPSAYVEKVNVGNLPAEYYQGVLFQDDQGNVTWQPNDPTV
                     TLRWADGGSSYTLFYFFQNQEDPLTMEELIAVAESMTTEPVAK"
     gene            complement(4390..4977)
                     /locus_tag="VFQ23_10525"
     CDS             complement(4390..4977)
                     /locus_tag="VFQ23_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9907069.1"
                     /translation="MNLFRQRNSAIASPASFRALYELNRLPVFRYTYALTNGSQADAE
                     DLTAETFLRAWKARHQFEGEMDSAIGWLIGIAKRLVIDEYRRTARASRNLPMDPDPVS
                     TPEQTAIHAEQQNFILRLVADLPYEQREIMVLRYLLGWRVKEIARYLGASENKISLTI
                     HRTLSRLKEQWINTDSETLSAVFTQKEITYEPNSR"
     gene            complement(5177..6727)
                     /locus_tag="VFQ23_10530"
     CDS             complement(5177..6727)
                     /locus_tag="VFQ23_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9907070.1"
                     /translation="MTYNSGRSRNLASYSRVWTCSILLFTLSACAQATQDVTVTEPPP
                     ATLSPTIEPTSLPPTSTAIIPSPTSPPLPESNLSFEAFAVPEGEVIALVNGRVIDGTG
                     APAWLNWTVLIQGTDIIEAGPNVVIPEGARRVDLTGQTLLPGLFDMHGHLYSYNGEKL
                     KAEFITYPRLYLAGGVTTLFTAGDFDPRGAISLREKIRNNEAVGPQILTAGPYFSGGS
                     APSWMLTARTPEEMRALYEVWNDQIDGVKTYTRIPEDQFVALLEAAHADGLKVTGHLE
                     SISGLRAIELGIDGIEHGLFSMSEFFPNLPQSVSFRAQYCAISQLDMTSSEVTVIVDA
                     LVNKGIYIDPTIVVFQPELPDFVPLVADWEKYLDPGAVMPLRRALRSIIEPTCLPTAL
                     EKQSQFVKAVHDRGGLIVTGTDPVLPILLPGYSLHRELQNLVNAGLSPLEAIKAATLN
                     AAIATGLDSQKGTIAAGKIADLVIVTGNPDEDITAIGNTVLVFKEGMPYLPEALRKSV
                     EGQIGKDD"
     gene            complement(6891..8126)
                     /locus_tag="VFQ23_10535"
     CDS             complement(6891..8126)
                     /locus_tag="VFQ23_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907071.1"
                     /translation="MDKKNIQAILKDELEMKIPSTDVRLWHAVKEDLVAGKTFQQGEK
                     MNTIYSRRIPRVLFVLLILVALFSLAFVTPQGRAFAQSILQFFTRAESNTFPLQPSQV
                     GQEGTDPSIPTAEPPAPILTMAEVEAQAGFDAAELPFIPKGFNYLGTRLYGQAISIEY
                     EAQGGGGNLIIMQSQAGFIQSEWDSVPAESIVPVKIGETDAEFAQGTFVVYPGETLAK
                     WNPDAPILRLRWVKDGIAFEVTKFGNVEAIQYLDQSALIQLAESLSNKPFAAPLSEVE
                     KRAGFDILEAAQPPDGMKFLGASFDPIYSLVSLSYGYSADDRRILIKQQPVSSGENCD
                     LCGIVGASAAVQIVQIGEVAGEYAEGVWELTDNGPVWRDDPYLKTLRWQKDGMAFELI
                     FMGVELEKDDLVDIATNLK"
     gene            complement(8129..8686)
                     /locus_tag="VFQ23_10540"
     CDS             complement(8129..8686)
                     /locus_tag="VFQ23_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9907072.1"
                     /translation="MDDQIAISRLKQGNLNGLEVLVNRYQAQAVRAAYLILYDRALAE
                     DVAQTAFVKAAERIHQFDEARPFAPWFFRIVVNDALKSAQHQRHISPLDDLDEPAARL
                     ADWLTDPVPRPEQQLEEKEIRENLLRAIHSLPPEQRAVIVMQYYLDLSEKDISTKMDR
                     PLSTIKWWLRDARKRLRDLLAYDRS"
     gene            8845..9765
                     /locus_tag="VFQ23_10545"
     CDS             8845..9765
                     /locus_tag="VFQ23_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9907073.1"
                     /translation="MNNNDAPKPDLTIVVPVYNEAESLTELLSTLVPTCRLHGWLLIL
                     INDGSRDGSDKILSTIETDPVVTILHHKVNRGYGGALKTGISAATTPYVITMDADGQH
                     DISDILKLFALAQTKNADMVIGARTNTKNVSLYRELGKWLIRRITNILVPLPIRDLNS
                     GLKLYQTKLVQRYLSLCPDSMAFSDVITLLFLNQRHLVLECEINIRHRTTGSSSVSTR
                     TAFQTVIEILSVVMLLNPLRIFLPLSILCVTAGLLWGIPIILLGRGVSVGSMLAIVLG
                     ALFFFLGLIANQLSAIRLHLAGMSKNENPE"
     gene            9749..11611
                     /locus_tag="VFQ23_10550"
     CDS             9749..11611
                     /locus_tag="VFQ23_10550"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HET9907074.1"
                     /translation="MKILSNIHRSRVELSLFLILLTVGVPMVLLIPPGAGYDEEDHLV
                     RVWEMSFFSFIPGQIPPQAMQYPKVFRDFAYRHQAGGMIDSDYWQNYSGVSLYERGSV
                     SREINTKSVYSPALLLPQAMVLRYVGRPADLPALTIFYLCRFAGLISYLVLIWFAIRL
                     IPFGKWILLVLALSPIAMFQATTISADAISNGLGFLFIAGCLNVAESKEINWKECGAL
                     VFLVFLLFLAKLNLVPLILLPFLLLLPSKFTNKGIYFFFLAMVFVLFVIEVAAWNRIA
                     SLQLDPVLANEANVTEQILYIFGHPFRFLQIIITDFITNGWAYFQGWINGYGYYYWTP
                     PAIVSLFYLLSLGSVLFVDSAAHFVEKRFQFAFILTFIASYFATILSLYLTFTPVGAD
                     EILGVQGRYFVPLVLLLFFLLSGVSGLKKFTVPSSKWTVGFLALALCLNILGIALAFY
                     VSCGTTYYQTGLCYEPFHKDFPAEARLSPPISDDMEVTQETQITCNGLTELRVLLKPS
                     LKETQGSTRFIFQDPLTGDNLFDESVSNDQIIKEDWYNLSFDPVWDSAGKQYHLIILG
                     TNTPPGQGFQLLYTPQPEFDLGTLYENGQPLEENIALQYGCVTGLRKIWLTRKP"
     gene            11649..12551
                     /locus_tag="VFQ23_10555"
     CDS             11649..12551
                     /locus_tag="VFQ23_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9907075.1"
                     /translation="MKTVLKWIGIVLGSLIGVVLFAGVVLYFIGNGRLNKTYEIPPSN
                     LVLPTDAASIEYGKHRVETLCQGCHGDDLSGINDWFSAPPIGTIDSANLTAGEGGVGA
                     EFTTEDYVRAIRHGIDREGKPIFMTAVPATAHLSDEDLASIIAYLKTIPPVDHQLDGR
                     YFTPLAKIMLAAGVLGKLPAEEVSHDVHVTAPERGVSTEYGKYLVDTNDCRVCHGPEL
                     NGGPFPDPTITKISPNLTPGGEVGFWSEEDFINALRTGTTPSGHQLDSEFMPWEAYRL
                     FYDDELKAIYLYLQSLPKLEQYTE"
     gene            12669..13247
                     /locus_tag="VFQ23_10560"
     CDS             12669..13247
                     /locus_tag="VFQ23_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907076.1"
                     /translation="MPASDPLEKLKNGRAAAIELIPILRRKLASRDGSVHPGTILSAA
                     AWLTGTSLYRAFHPKDESPPATTIKSDEVNQEWESLMYLFEQYNFQKADIPVGYLIMA
                     ALASDEAHKPKVEMIYVQRELQDQYNAIMRKHGFNYADAARAGVVLCSLLSQLHSTNL
                     KEIDLSVAAGIVAQGILEAAKTVPPPLKTEGR"
     gene            13255..13611
                     /locus_tag="VFQ23_10565"
     CDS             13255..13611
                     /locus_tag="VFQ23_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907077.1"
                     /translation="MTSTAKSIFYYSFYMISVGLGLLFVPGLILNVVGFEPTNDLWIR
                     ILGLFAFCAGMLYFYCGQTNQTGFFRISVPERIVFFLGMVGIVLFLPANPLLFLIGSV
                     DLFGALWTALTLRSST"
     gene            13664..>14230
                     /locus_tag="VFQ23_10570"
     CDS             13664..>14230
                     /locus_tag="VFQ23_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907078.1"
                     /translation="MRIQNFSSDVRAWLDDLPIPDPVDRRMAALLQFILLGFMGIILL
                     ALILNPILGADLSPQDLQTTMVANSIGVVVLALPMVLLRRGYFHASVLIVIGILFVFS
                     TLSVVLAMSLRETNGTLFPLTFSLILAGLLVGKRALLVTFVLSIGVLMLGASLERSSD
                     PELRLNNIAIAVNFVLFNGLMCLFLHRFG"
BASE COUNT         3482 a         3462 c         3483 g         3803 t
ORIGIN      
        1 tgtgccgggc aaaggctcca cattttattt cacactgtca cgcgggtagt gccaaaatgt
       61 acactggcaa ttgggttgat tacgaaacga tgatgcaggg gaaattgggg aaagactttg
      121 actcccacac cgagtgaagt acagaacgtt gaaaagaaaa aataatagac cagcgacatt
      181 cgccggtcta ttacacaagg gctggttatg cctttggctt tttgctgatt tcccatacgc
      241 aggctttggc gcccatggcc cggcattcca cttcctgcac atcatattct ttttccatga
      301 gccatagggt tgcttcgctt atgttgccag tgaatacggt acaaatcgcg gcatcggctt
      361 cacggtcaat acataccgta cagtcttcca caatgtatgc gaatttttcg ccgcggtctt
      421 caatgtgggc gtgccaatcg atgccaacgt ctttatagag cttcttgaaa atgttgaggt
      481 tggattccaa cccgcccttt tgcttcatcc cggcgggcaa cagccgcgct gcggctacca
      541 tggcgctcat gcccatcgaa tccatataat tgttgatgaa catgcgcgcc gaaagtttgc
      601 caattcggat gagaccgccg cgtccagcgc gtccaaaaaa ggtgagcaac ccggtgcata
      661 atgccgtgta atctttttgc agagcgccag ctgaagcatt catttcttct gatggataat
      721 tgtcaatgaa acgctccagt ccgttctcgc gcaaaacaat agtcaaaccc tgcttgcccg
      781 ccacttcttc ggccgcgagc aaggcccaac gaaaaaacgc gttgtatatt cctttgtcgc
      841 ctaatggttc atgtgtgggt ttgactttga tttccatatc ccattctcct tctgttgaaa
      901 atcgaagcgt ctatgagagt atactccttt tagtaagttt ttcaagactt tatagtcgtc
      961 attctttgta gtcaacggga ttgggttggt tatgatggga agcgaaggac ttttcggcaa
     1021 ctggtcccat tacgagaagt tcaaatacta cattaaactg acggtcacgt ttgaacgtga
     1081 ccgtcagttt ttataacttc tcaatcgcag gccgggtcag gagttggtac atttcattca
     1141 tcagctgggg cgggtcggac atatcgtgat tgacccacca gatcagcagt gataccagag
     1201 aactgaccag atgataaacg accacctcca gggggatctg tctgtctttc gtccactgga
     1261 ctttcaatga atcccgcatg agcgctgcaa gataacgatg catatgggca gacattagtt
     1321 tttcacccgg cctgcccacc agcgcgcgat agataccacc atatgcctga gcatgctcga
     1381 acacgatcag gctgagttcc caaacgctgg ctttttgagc cgcctgggcg gctagatgct
     1441 gatcgaacaa cgaccagaga ttctcgaatc cgctcaggaa caagtcctcg gtgtccttga
     1501 agtgcgaata gaacgtggaa cgcccgacgt tggcgcgctg gatcacatcc tgcaccgtga
     1561 ccgcctcgaa ctccttttcc aggatcaact ccagcagggc ctcctgcaat aatttgcgcg
     1621 tgcgctggat ccgtcgatca aaagtctcat tccgcatcct tgtctctcct tgttcctgga
     1681 caaaacaggc tggaatgtcc ggtattgaac acttccattt tccatgtttg ttgacggtca
     1741 gagtcccggt cagtataaca aaaaggaaca ccgggttcaa aaccaaatca tcttacagga
     1801 gatacaaatc atggaaaaca agaacacagc agtggtgaaa agttttttgg acgctctctt
     1861 ttcgggcggt actgcagagg catctgaaat tcttgcagac aatctcaagg tcgataactt
     1921 cttcccacac cagagtagta agaaagcctt tctgcgggcg tttcaacagg tattaaaagc
     1981 catggaaggt gtacaggtcc aattgaacgc cctgcagggc accgaaaata tggtcaccgc
     2041 tggtttccag gtcagcggga ttcatgccca accgcttgat ttcgctttta tgaaattacc
     2101 ggtcgtccaa cccacccaca aaaccgttgc atggcctgcg ctgcactggg actttaccat
     2161 ttcaaacggg aagatcgtga acattcggaa cacagcctcc ttgcagaagg gcatgccggg
     2221 aattttgaaa tcgttcggtg tcaagatgcc ggccgcgcat aaatttggaa gcgtgctcaa
     2281 agccctcgct ccagctggca aactcaaaac gaaccccaag cgttttctgc gcttcctgcc
     2341 attgattcct ttaactgtga tcctgaccac gatatttttc gtcccctccc cgatcaacgc
     2401 tgccgcatgg acaccgcaac ctgtcccgca ggcgaagggt ccactgacta caaattatga
     2461 actgacagaa aagggcgaat tgcttggggc gagtcagcta cagcatgccg aagacctcgc
     2521 ctttgatatg gatggcagaa tctacgcagg ctccgatgac gggaatatct atcgactcac
     2581 tttggatccc gcgggtcaag tctctcaatt tgaagccttt gccacgaccg gcggctttcc
     2641 cctcgggctc caattcgacg ggtccggcca gctgatcgtg gccgtcaagg acatcggctt
     2701 gatgtcaatt tcaaaagacg gaacagctgc cttgctaaca gatcaagtag aaggaacgcc
     2761 catcacgtat gcgaacgcca ttgccataaa cgctgatgga ctgatctact tcaccgactc
     2821 aagcacaaaa tttgatcggg gctggccgta tgatgtgctg gaagcccgcc cacatggccg
     2881 cttgttatct tatgacccgg aaaccggcga aagctccctc ataaaagacg ggctgtactt
     2941 tgcaaacggt atctttttgg cacctgatga aagttatctg ttgatcaacg aatcgtcacg
     3001 cgcgcgaatt tcccgctact ggctaacggg caaccaggca ggcgagctgg atacttttgc
     3061 ggaaaacctg cccctgcttc cagacaatat ctcggtggat gaaaatggaa actacctgct
     3121 gggcggcatg cgacgcctgc caatgatgga taagttgcag ccgaatgact ggctcaagaa
     3181 tcagatcgcg aaactccctt tacccatgct tagcaaattc ccgagcctca agatgaatcg
     3241 ctatggaatg atcctcatct tggatcagaa tggcaagatc aaggaaagtt tgcaggaccc
     3301 caccggaaaa attttcgcca tcagttcggc gcgagcctac gatggatata tttatatcag
     3361 cacactgctt ggcagtgata ttgcccggta tccatatcat cgctaaaagg aatcgatcga
     3421 gccggtctca ttacgagacg acgatgcaca aaagtattga aagactctaa cgtcgcagcg
     3481 agtacaatag cggacgttga aacggtaaac caaagtggca gtcaccaacg cgttgactgc
     3541 cactttttaa ttcagaccgt gtgatagcaa ttaacttgtc ggagaagaag agatgaggac
     3601 cttccgctcc tctctttgac tatttatttt gcaaccggtt cggtcgtcat actttctgcc
     3661 accgcgatca gctcttccat cgtcaaaggg tcttcctgat tttgaaagaa gtagaagagg
     3721 gtgtaactgc tgccaccatc cgcccagcgt aacgtcacgg tagggtcatt cggctgccat
     3781 gtgacattgc cctgatcatc ctggaaaaga acgccctggt aatattcggc gggcaggttt
     3841 ccgacgttta ccttttccac ataggcactg gggggaacga tgtgcagagc accaggagca
     3901 ggtctgccgg tggcttccac gagtataccg atcccacctg caagtctgtt cggatattca
     3961 taatggaggt caacccaatc cggtcctccc gtggcgccca catagtacag gtcaccagga
     4021 agagttccca gttccttgac ttcaaaatcc accatgctgc ggatctcttc gacggaacat
     4081 gtggggcgga tgggaatgcc gcactgctga tgaaatgggg tcgtttcttc aaaagtcagg
     4141 tttgataccg gctcatagta tgaatcgctc tgtgtacggg tgaagaactg cagaacatcc
     4201 tgcgcaaagg catgaccctg tgggctggcg gcaaagacag aagctaccag gagcacagca
     4261 agggatatcg cttgcaagga acggcgacga ttgacgactc cttcgctcca tggagcatcg
     4321 gccagtttcg tttcgaggcg cgaagtagta tggggtgcta tgctttcgag gagttcttcg
     4381 atttgttttt catctggaat ttggttcata agttatttcc ttttgtgtga acaccgctga
     4441 aagagtttct gaatcggtgt ttatccattg ttctttcaac ctggaaagag ttcggtggat
     4501 ggtcagggag atcttgtttt ctgaagcgcc caggtatcgg gcgatctcct tcactcgcca
     4561 gcccaacagg tagcgtaaga ccataatctc acgttgctca tagggaaggt ccgccacaag
     4621 gcgaaggatg aagttctgct gttctgcatg aatagcagtc tgttccggcg tggacacagg
     4681 gtcaggatcc atcggcaggt tgcgggaggc gcgagctgtt ctacgatact catcgatgac
     4741 cagtcgtttg gcaatgccga tcaaccagcc gatggctgag tccatctcgc cctcaaattg
     4801 atgccgcgcc ttccaggcac gcaggaaggt ttcggcggtc aggtcttctg catccgcttg
     4861 ggaaccgttc gtcagggcgt acgtatagcg aaagaccggt aatcggttga gctcatacaa
     4921 tgccctgaag ctggcggggg atgcaatcgc ggaatttcgt tgacggaata gattcataaa
     4981 tctcctgttg aacgctgaaa cgcgcgtgtt tcactatgaa aacgctgatc tcgagaaaat
     5041 ctttctaggc agggtactga attattggct catagtgaaa aacagttctt gagaactgca
     5101 attaggcggg agagttatga gattggtaat atcccccata tgaacacata cagattatca
     5161 acttgagacg acccgtttag tcgtctttcc cgatctgccc ttccaccgac ttgcgcaatg
     5221 cctcgggcaa atacggcatc ccttctttga aaaccagaac tgtgttgccg atggcggtaa
     5281 tatcttcatc cggattgccc gttacaataa ccaggtcggc gatctttccg gcggcgattg
     5341 tgcctttttg cgaatccaat ccggtcgcaa tggcggcatt aagagttgct gccttgatcg
     5401 cttccaaagg ggacaaacca gcattcacta gattctgtag ttcacgatga aggctataac
     5461 ctggcaggag gataggaagt accgggtccg ttcctgtaac gatcagccca ccgcgatcat
     5521 ggacggcttt gacaaactga gattgttttt ccagcgcggt aggcaggcag gtaggctcaa
     5581 taatggaacg aagcgctctg cgtaacggca ttacggcgcc agggtcgaga tacttttccc
     5641 aatcggccac cagcggcacg aaatcaggca actcgggttg aaacaccaca atcgttgggt
     5701 cgatgtagat acccttattc acgagcgcat ccacgataac cgtgacttcg gaagatgtca
     5761 tgtccaattg gctaatggcg caatactgtg cccggaatga aacactttga ggaagattcg
     5821 gaaaaaattc agacatggaa aaaagcccat gctcaatacc atcaataccg agttcaatag
     5881 ccctcaggcc cgatatcgat tcaaggtgtc cggttacttt caatccgtca gcatgtgcag
     5941 cttcaagcag cgcaacgaac tggtcttccg ggattctggt ataggttttt accccatcga
     6001 tttgatcgtt ccacacctca tataaggccc gcatctcttc gggagttcgg gcggtcagca
     6061 tccaggaggg agcgctgcca ccactgaaat atggaccggc tgttaagatt tgtggtccca
     6121 cagcctcgtt gttcctgatt ttttctctta atgagatggc gccgcgtgga tcaaaatcgc
     6181 ccgctgtaaa gagcgtggtg acgccgcctg ccagatagag ccggggatag gtaatgaatt
     6241 ctgcttttaa cttctcaccg ttatatgaat aaaggtgccc atgcatatcg aacaatcctg
     6301 gcaggagagt ttggcccgtc agatctacac gtcgggcccc ttctggaatg acgacgttcg
     6361 gacccgcctc tataatgtct gttccttgaa tgagaacggt ccaatttaac catgctggag
     6421 cacccgtgcc atcgatcacc cttccgttga ccaaagcgat gacttcaccc tcaggtacag
     6481 caaacgcctc aaatgagaga ttagattcag ggagcggagg agaagtggga gacggaataa
     6541 tggcagtgct tgtcggtggc aggctggtcg gttcaatcgt gggagatagg gtagctggtg
     6601 gtggttcggt gaccgtaaca tcctgcgttg cctgggcaca cgcggaaagt gtgaataaaa
     6661 ggatggaaca cgtccataca cggctgtagc tggccaggtt tcgagatcgt ccagagttgt
     6721 aagtcatcgc taataagttc cagaaggtta ttcggatgcg tcgaatgtaa tgttacccca
     6781 gaatgatttc gtttgcctgc aggaagtaat tactgatggc cttttgcgaa tgtaatcgtt
     6841 tgttaaaaat agacagtcac ttgagtggct gtctattttt attttgctgg ttacttcaaa
     6901 ttcgtggcga tatcaactaa atcatctttt tctaattcca cgcccataaa gatgagttca
     6961 aaagccatgc catctttttg ccagcgcaga gtcttgagat acgggtcatc tcgccagacc
     7021 ggaccgttat cggtcaattc ccaaacgcct tccgcgtatt cgccagctac ttccccaatt
     7081 tgaactatct gcacagcagc gctggcgccc acgatgccac ataagtcaca attctcacct
     7141 gagctcaccg gctgttgttt gatcagaatg cgacggtcat ctgccgaata cccataggaa
     7201 agcgaaacca ggctatagat ggggtcaaag gatgcgccga ggaatttcat tccatctggg
     7261 ggctgggctg cttcgaggat atcgaatccg gcccgttttt caacttcact caggggcgcc
     7321 gcgaagggtt tgttgctaag gctctcggcc agttggataa gagcactctg gtcgagatat
     7381 tggatggctt ctacatttcc aaactttgtt acttcgaatg cgataccgtc tttcacccag
     7441 cgcaggcgca gaatgggcgc atcaggattc cattttgcta aagtctctcc gggatagacc
     7501 acgaacgtgc cttgcgcgaa ctctgcgtcc gtttcaccga tctttaccgg aacaatagac
     7561 tcagctggaa ccgaatccca ttctgattga atgaagcccg cctgcgattg cataatgatg
     7621 agattaccgc cacctccctg tgcctcatat tcaatactga ttgcctgacc atacaatcgt
     7681 gtgccgaggt aattgaaccc tttcggaatg aagggcaatt cagcggcatc aaaacctgcc
     7741 tgcgcttcta cttcagccat agtgaggatc ggggctggtg gttcagccgt cgggatggat
     7801 ggatcagttc cctcctgtcc aacttgcgaa ggttgcagcg ggaaggtgtt actctccgca
     7861 cgggtgaaga actgcagaat gctttgagcg aaggcgcgtc cctgtggtgt gacaaaagcc
     7921 aacgagaaaa gcgcgacgag gatcaacagt acaaacaaaa cgcgcggaat gcgacgtgaa
     7981 taaatggtat tcattttttc tccttgttga aaagtttttc ccgcaacaag gtcttctttc
     8041 acagcgtgcc agagacgaac atcggtggaa ggaattttca tttccagttc atccttcaaa
     8101 atggcttgga tgtttttctt atccatggtt aactcctatc gtatgcaagc aggtcacgaa
     8161 gtcgtttacg tgcatctctc agccaccatt tgatggtgct caaaggacga tccatttttg
     8221 tggagatatc cttttcgctc aaatccagat aatactgcat cacaatcact gcacgctgtt
     8281 caggcggaag gctatggatg gctcttagga gattttcacg gatttccttt tcctccagtt
     8341 gttgctcagg ccgaggaaca gggtcagtca accagtctgc gagccgtgcc gcaggttcat
     8401 ccaaatcatc caatggagat atatgccttt gatgttgcgc agatttcaac gcgtcgttca
     8461 caacgattcg aaaaaaccag ggagcaaacg gacgtgcttc atcgaattga tggattcgtt
     8521 cggcggcttt tacaaacgct gtttgtgcaa catcctctgc cagagcgcga tcatagagga
     8581 taagatacgc ggcacgtact gcctgtgcct gataccggtt aacgagcact tccagaccgt
     8641 tcaagttgcc ttgttttaag cgggaaattg caatttgatc atccatgcat ggacctttgg
     8701 gagaattctc tacttatact atcccttccg ggggaaaaag atagcagctt tgccaaactt
     8761 catagcgtgg gcgtagcaag caaaagaatc aggatcgatg aagggctgac tacaaaacat
     8821 tgttagaggt agaatcacct cgacatgaac aataacgacg ctccaaagcc ggacctgaca
     8881 attgtggtcc cggtctataa tgaggcagaa tccctgactg aacttttgtc tacactcgta
     8941 ccaacctgtc ggctccatgg ctggctgctt attttgataa acgatgggtc aagggatggt
     9001 tccgacaaga ttctctctac aattgaaacg gatcctgttg taacgatcct gcatcacaaa
     9061 gtcaatcgag gatatggagg cgcgctaaaa acagggatat ctgctgcaac caccccgtat
     9121 gtgatcacca tggacgcaga tggccagcat gatatttctg atatccttaa attatttgct
     9181 ttggcgcaaa cgaaaaatgc agatatggtg attggagccc gtacgaatac aaaaaatgtc
     9241 agcttgtatc gggaactggg gaaatggtta atacgaagaa ttaccaatat tcttgtacct
     9301 cttccgataa gggatttaaa ttccgggctc aagctttatc aaaccaaact ggtgcagcga
     9361 tatctttcct tatgcccgga ttccatggca tttagtgatg tcattacgct tttgtttctg
     9421 aaccaaagac atcttgtcct ggaatgtgaa atcaacatac ggcatcggac gaccggttcc
     9481 agctccgtca gtacgcgcac tgcatttcag acagtgattg aaatcctcag tgtagtgatg
     9541 ttgttgaatc cgttaaggat attcctgcca ttgtcgatac tttgtgttac tgcagggctg
     9601 ttgtggggaa ttccgatcat attattaggt cgtggggtca gcgtggggtc gatgctggcg
     9661 atcgtgttgg gagcgctttt tttcttttta gggcttattg caaatcaatt atcggcaata
     9721 aggcttcatc ttgccggcat gagcaagaat gaaaatcctg agtaacatcc atcgttcgag
     9781 agtcgaactc tccctttttt taatattatt aacagtcggg gtgcccatgg ttttattgat
     9841 cccaccgggt gcgggatatg atgaagaaga tcatctcgtc cgtgtctggg agatgtcctt
     9901 tttctctttc ataccggggc aaatccctcc gcaggccatg caatatccaa aagtattccg
     9961 agattttgca taccggcatc aggccggtgg catgatcgat tccgattact ggcaaaacta
    10021 ttccggggta tcgctatatg aacgcggctc ggtaagcagg gagatcaata ccaaatcggt
    10081 ttattcccct gcgcttttgc tgcctcaggc gatggttctg cgttacgtgg gtcgcccggc
    10141 tgacctaccg gcgcttacta tattttattt atgccgcttt gcaggtttga tcagctattt
    10201 ggtcctgata tggtttgcga ttcggctgat cccgttcggg aaatggattc tgctggtttt
    10261 ggcactctct cccattgcga tgtttcaagc caccaccatc agtgcggatg ccatctcaaa
    10321 cggcctaggg tttttgttta ttgcgggatg cctcaatgtg gcggagtcga aagaaatcaa
    10381 ttggaaggaa tgcggggctt tagtttttct tgtgtttctg ctttttctcg cgaaactaaa
    10441 tctggtgccg cttatcttgc ttccattcct gctgcttctg ccctcaaaat ttacgaataa
    10501 gggaatttat tttttcttcc ttgccatggt ctttgttctc tttgtgattg aagttgcggc
    10561 atggaaccgg attgcatcgt tacagttgga tcctgtgctg gcaaacgaag cgaatgtcac
    10621 tgaacaaatc ctctatatat ttggtcatcc gtttagattt cttcaaatta tcatcactga
    10681 cttcatcaca aatggatggg catacttcca gggctggatc aacggttacg gttactatta
    10741 ctggactccc cctgcgattg tgtccctttt ctatctcctg agcctgggct ctgttttgtt
    10801 tgtagactca gctgcacatt ttgttgagaa acgctttcag tttgcattta ttctgacatt
    10861 tatagcaagc tactttgcta cgatcctgtc actatattta acctttacgc ctgtgggtgc
    10921 agatgaaatc ctgggtgtgc agggacgcta ttttgtaccg ctggtattac tgctcttttt
    10981 cctcctttca ggtgtttcgg gtttaaaaaa attcactgta ccttcctcca aatggacagt
    11041 tggtttttta gctctggctc tttgtttaaa tatcctggga atcgccctgg cattttatgt
    11101 gtcatgtgga acaacctact atcaaaccgg gttatgctat gagcctttcc ataaggattt
    11161 ccccgcagag gcgcgcctct ctccacccat ttcagatgat atggaggtca ctcaggaaac
    11221 ccagatcaca tgtaacgggt taacggaatt acgcgtgttg ttgaagccct ccttgaagga
    11281 aactcaggga agtacgcgct tcatctttca ggacccattg actggtgata acttattcga
    11341 cgaatcggtt tcaaatgatc agataatcaa agaagactgg tacaacttga gctttgaccc
    11401 ggtttgggat tcagcgggga aacaatatca tttgattatt ctcggcacaa atacaccacc
    11461 tggccaggga tttcaactcc tttacacccc gcaaccagaa tttgaccttg gaacactcta
    11521 tgaaaacgga caacccctgg aggagaacat cgccctgcaa tatggttgtg ttaccggttt
    11581 acgaaaaata tggctgaccc gcaagccata aatagaaaat cattttttaa taaaggagaa
    11641 caacgatagt gaagacggtg ttgaaatgga tagggatagt gttaggttcg ttaattggag
    11701 tagtgctttt cgctggtgta gttctatatt tcatagggaa tgggcgcctc aacaagacat
    11761 atgaaattcc accctccaat ctggttctcc ctactgatgc agcaagcatt gaatatggta
    11821 aacatcgcgt cgagaccctg tgccagggtt gtcatggtga tgatctaagc ggaataaatg
    11881 attggtttag cgcgccgccg attggcacaa ttgattcagc aaacctgaca gcaggcgaag
    11941 gcggagtcgg agccgagttt acaaccgaag attacgtccg ggcgattcgg catggcattg
    12001 accgtgaggg caagccaatc ttcatgaccg ctgtccctgc gactgcccat ttgagcgacg
    12061 aagatttagc ctccattatt gcctacctta agaccattcc acctgtagat catcaactgg
    12121 atggaaggta tttcactcct ttggcaaaga tcatgctagc agcaggtgtg ctgggaaaat
    12181 taccggcgga agaagtgagt cacgatgtcc atgtaactgc gcctgaacgc ggcgtcagca
    12241 cagaatatgg taaatatctg gttgatacga atgattgccg tgtttgtcac ggtccggagt
    12301 tgaatggtgg cccattccct gaccccacta tcacaaaaat atccccgaat ctgactccgg
    12361 gcggagaagt cggcttttgg agcgaagaag atttcattaa tgccctccgc accggcacaa
    12421 cacccagcgg ccatcaactg gattcagagt ttatgccctg ggaagcgtat cgtctgtttt
    12481 atgacgatga attgaaagct atctatctgt atttgcaatc tctgcccaag ttggaacaat
    12541 atacggaata gagagcaact aaagcttttc tgatcgtggt cggagtattg atttattcca
    12601 taaagtttgg aataatcact tcgtctcaat tagtgagatt tcagtccaca tgtggaggag
    12661 tatctttcat gccggcctct gacccgctgg aaaaacttaa aaatggtcgt gccgcggcaa
    12721 tcgaacttat tccaatctta cggcgtaaac ttgcttcccg ggatggtagt gtccatcccg
    12781 ggacgatcct ctctgccgca gcctggctca ccggcactag cctctaccga gcctttcacc
    12841 ccaaagatga aagtccgccc gccacaacca taaaatcaga tgaggtcaat caagagtggg
    12901 aaagtctcat gtaccttttt gagcaataca attttcaaaa agcagatatt ccggtggggt
    12961 atttgattat ggcggcttta gccagtgatg aagcacataa gccaaaggta gaaatgattt
    13021 atgttcagag agaactgcaa gatcaataca atgcaatcat gagaaagcac gggtttaatt
    13081 acgccgacgc tgcccgggct ggtgtggtgc tttgttctct tttatctcag cttcactcaa
    13141 ccaatctcaa agagatcgat ctctcggtcg cggcgggaat tgtcgcgcag ggcattttgg
    13201 aggccgcaaa gacagttcct ccacctttga aaaccgaggg acgttaagaa cctgatgacc
    13261 tccacagcga aaagcatctt ttattacagt ttttacatga tcagtgtggg gctgggattg
    13321 ttatttgttc caggtctgat tctcaacgtt gttggttttg agccgacgaa cgacctctgg
    13381 attcgcattc ttggactgtt tgctttttgc gcgggcatgc tttattttta ttgtgggcag
    13441 acaaatcaaa ctggtttctt tcgtatttca gttcccgagc gaatcgtttt ctttctggga
    13501 atggtaggga tcgttctgtt cctgccagcc aatccgctac tctttctgat cggtagtgtc
    13561 gatttattcg gcgcactttg gacagcgctc acgctgcgta gttcaacgta atcacaataa
    13621 atactagcag gttcatacaa ggtaaaattt cgggcagagt tcgatgagga ttcaaaactt
    13681 ctcgtctgat gtacgcgcct ggctggatga tcttcccatt cctgatcctg tggatcgccg
    13741 gatggcggct cttctccaat tcatcctgct cggcttcatg ggcattattc tcctcgcgct
    13801 tattctaaac cccattcttg gtgccgatct ttctccgcag gatttacaaa ctaccatggt
    13861 tgcaaattcg attggcgtgg tggtcctggc gctgccgatg gtattactgc gccgtggcta
    13921 ctttcacgcc tctgtgctga ttgttatcgg gattctgttt gtcttttcta cgctgtcggt
    13981 ggtcctggcg atgagtcttc gcgagaccaa cgggacattg tttccgctga cattttccct
    14041 gatcctggca gggcttcttg taggcaaacg tgcgcttttg gtcacctttg tgctaagcat
    14101 tggcgtgctc atgctgggcg cctctctgga acggagttcc gaccctgagt tgagacttaa
    14161 caatatcgcc attgcagtga attttgtttt gtttaacggt ttgatgtgtc tctttcttca
    14221 tcgtttcgga
//
LOCUS       DASTJP010000354         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_641108, whole genome shotgun sequence.
ACCESSION   DASTJP010000354 DASTJP010000000
VERSION     DASTJP010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641108"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..2024)
                     /locus_tag="VFQ23_10575"
     CDS             complement(123..2024)
                     /locus_tag="VFQ23_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET9907079.1"
                     /translation="MNPSFRQKFQYHFDNLMSRGTPAMVGMLFLLSLIIVSIAGAIIT
                     IAGFVQEGETARLSFGEATWESLMRTLDSGTMGGDTGAGFRGVMLFVTLGGIFIVSAL
                     IGVLSNGLEAQMDRLRKGRSLVLENNHSLILGWSAQIFTVINELMVANENQGNARIVI
                     LADKDKVEMEDEIRERVEIRGKTRIICRSGSPIDPNDIEIASPHHAKSIIVLPPENAD
                     PDTDVIKTVLAITNNPNRRAEKYHIVTQIRHAKNLNVLKMVGANDNVQAILTGDLIAR
                     IVAQTSRQSGLSVVYTELMNFGGDEIYFKHEPGLAGKTFGEALLAYEDSCVMGLRKAD
                     GRILLSPPMDSQIESGDQIFAIAADDDTIRLSGSGPAPVDESIIRSSREPLNPKPEKC
                     LILGWNRSGTTIVHELDNYVPSGSQVTVVADVYNIEKQIRAQGGKLKNQKLVVQEGET
                     TDRDLLNELHVEDYDHVIVLAYSTLEPQEADAKTLVTLLHLRDMAEKDATPFSIVSEM
                     LDLRNRELAEATQVDDFIVSEHLVSLMMSQLSENAALFDVFTDIFDPEGAEIYLKPVS
                     DYVATGQPVNFYTVIEAARRRGETALGYRIESESSDSEKAYGVHTNPKKSEKVRFAAE
                     DKIIVIAEE"
BASE COUNT          537 a          599 c          543 g          553 t
ORIGIN      
        1 gttgaacgtt ctctactaat aatatcccca gggattgaaa aacgatagtt tgccggtggt
       61 tccaagctgt tcaataattc cccaaagaaa aaagagcgag gccgcggcct cgctcttcaa
      121 gattactctt cggcaattac gatgatctta tcttcggcgg caaaccgcac cttctccgat
      181 ttctttggat tggtgtgtac tccatacgct ttttctgaat cactcgattc agactcaatt
      241 cgataaccga gagctgtttc ccctcgtcga cgcgcggcct cgatcacagt ataaaaattg
      301 acaggctgcc cggtcgccac ataatcactc accggtttga gataaatctc tgcgccttca
      361 gggtcaaaga tatctgtgaa gacgtcaaat agcgcagcat tctcagagag ttgagacatc
      421 atcaaactga caagatgttc gctgacgatg aaatcatcca cttgagtggc ttccgctagt
      481 tcgcggttgc gaagatcgag catttcgctg acgatcgaga agggagtggc atctttttct
      541 gccatatcgc gcaaatgcag taaggtgacg agcgtcttcg catcggcttc ctgtggctcc
      601 agcgtgctgt acgccagcac gatcacgtgg tcataatcct ccacatgaag ctcattcagc
      661 aggtcgcggt cggtggtctc gccttcctgt acgaccagtt tctgattctt cagcttccca
      721 ccctgtgccc ggatctgttt ttcgatgtta tagacatccg ccacaaccgt cacctgcgaa
      781 ccagagggaa catagttatc aagttcatgc acgatggtgg taccactgcg gttccagccg
      841 aggatcaggc acttctcggg tttgggattt agcggttccc ggctggagcg tatgatcgat
      901 tcatcaaccg gagccggacc agaacctgaa agacgaatcg tgtcgtcgtc agccgcgatg
      961 gcaaagatct ggtcaccgct ttcaatttga gaatccattg gtggactgag cagaattcgt
     1021 ccatctgctt ttcgcaaacc catcacacaa gaatcctcat aagccaacag tgcctcaccg
     1081 aacgttttgc cggccagacc cggctcatgt ttgaaataaa tttcatcgcc gccgaaattc
     1141 atcagttcgg tgtaaaccac cgagagaccc gattggcgcg acgtctgcgc gacgatgcgc
     1201 gcgatcaaat caccggtaag gatagcctgc acattgtcat ttgcaccgac catcttgagc
     1261 acgttgagat tctttgcatg acgaatctgc gtgacaatgt gatatttttc cgcgcggcga
     1321 ttgggattat ttgtgatcgc cagcacggtc ttgatcacgt cggtatccgg gtcggcgttt
     1381 tcgggcggca atacgataat ggatttggcg tgatgcgggc tcgcgatctc gatgtcattg
     1441 gggtcaatgg gacttccact gcggcaaata atacgggtct tcccgcggat ttcgacccgc
     1501 tctcgtatct catcttccat ctccactttg tctttatctg ccaatataac aatgcgcgcg
     1561 ttgccctggt tctcgttcgc gaccatcagt tcattgatca ccgtgaagat ttgcgccgac
     1621 cacccgagga tcagcgaatg attattttca aggaccaacg aacggccctt gcggagccga
     1681 tccatctgtg cctcgagccc attactcaag acaccgatca acgcgctgac gataaaaatc
     1741 ccacccagtg tgacgaacaa catcacgccg cggaagcccg caccggtgtc gccgcccatc
     1801 gtgccggagt caagggtgcg catcaggctt tcccaggtgg cttcgccaaa gctgaggcga
     1861 gcggtttcac cctcctgtac aaacccggca atcgtaatga tcgcgcccgc aatggaaaca
     1921 atgatcaggg aaagcaggaa caacattccc accatcgccg gcgtgccgcg agacatcagg
     1981 ttatcaaagt gatattggaa cttctgacgg aacgatggat tcataaggac tccgtgtcac
     2041 ctaattaata taataattct atcttatcaa aggagactga tatctctgca aaattaacat
     2101 ggtatggaca tgccacactt ggactcgaaa cgggtggaca taaaattctt gtagatccat
     2161 ttgaggaagg gctggacctg gccgtcgttc ctatcggtga taatttcaca atgggtgtcg
     2221 atgacgcatt gc
//
LOCUS       DASTJP010000355         9614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1410684, whole genome shotgun sequence.
ACCESSION   DASTJP010000355 DASTJP010000000
VERSION     DASTJP010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410684"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..938
                     /locus_tag="VFQ23_10580"
     CDS             177..938
                     /locus_tag="VFQ23_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET9907080.1"
                     /translation="MKIMTWNVNGLRAVLGRNGLDWAWAAQPDILCLQEVKARPEQLK
                     EEYRNFPGYDVIWNPALKAGYSGVATFLRSASLETQLGMDAPLFDVEGRIISTLHPGF
                     RLFNVYFPSGQRGMDRVNYKLDFYAHLLDVCDRLHAKGESVVIAGDFNTAHMPIDLKN
                     AKQNSKTSGFLPEERAWVQKIIDHGFVDAYRSLYPDRIQYTWWTYRLSARERGIGWRL
                     DYFLVSEKLMPRVRDVIIHDSVAGSDHCPVELVLE"
     gene            1080..2960
                     /gene="ftsH"
                     /locus_tag="VFQ23_10585"
     CDS             1080..2960
                     /gene="ftsH"
                     /locus_tag="VFQ23_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9907081.1"
                     /translation="MNPRNQSFVVTFLLIVAAIAMVVMAVNRESTTEESLTINEVAAD
                     IIAGRVARVVIEDGDSLRVIYKTGGEAQTRTGLESYKEANSTLVEQLLALGVSAEQLS
                     AENVTIEVRPPSQWAGILSGALYILPVIFMAGVLWFIFRQAQGSNNAAMSFGKSRARM
                     FSGEHPTVTFQDVAGVEESKQELAEVVEFLKEPQKFIQLGARIPKGVLLVGPPGTGKT
                     LLAKAVSGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIVFVDEIDAVG
                     RQRGAGLGGSHDEREQTLNQMLVEMDGFDTDTNIIIIAATNRPDILDPALLRPGRFDR
                     RVTLDRPDMKGREAILKVHVKGKPLEPAVDLGSLARGTPGFVGADLENLVNEGAILAA
                     RRNKKSIGQQDLEEAIERVVMGPERKSRLISDEEKRIIAYHEAGHAVVMNAIPEADPV
                     QKITIVGRGQAGGLTWYRPEDDRILTSRKKMIAALAGLLGGRVAEELVFDDVTSGASS
                     DIERVTQMARTMVTRLGMSNDMGLMTYGQKEELIFLGREISEQRDYSEAIAEKIDAEV
                     RKLVDDAYKLTKKLLLKYRDRLDAVAQKLLEVESLTREEFEGIFPPPFPKKSGTPQVI
                     AM"
     gene            complement(3054..4757)
                     /locus_tag="VFQ23_10590"
     CDS             complement(3054..4757)
                     /locus_tag="VFQ23_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET9907082.1"
                     /translation="MNEKPWLAHYDKGVPHTVEIPSAPLFHLLEESARKYPDRACTIF
                     KGAVITYKEMNELTDRVAAALVDMGVKRGDRVGIFMPNTPQFVMAYYGILKAGGSVVA
                     TNPLYTPPEIEHQASDAGIEVMFVMTNFYKTIKKAQPNTKIKKMIVTNLKETLPPVMR
                     LLFTLLREKKGGFRIEGGLQEGEVWLQDLLAKYKGKSRPNIPIDPEDTALFQYSGGTT
                     GVSKGAVAAHRNVLANTLQMRSWFVNMEEGKEVFLMGIPLFHVYGMVAGMNLAMATGA
                     TMVMVPNVRDLKDVLENISKYRCTIFPGVPALYNGINNHPEVKAGKYDLSSIKACISG
                     SAPLMRETKEQFEKLTGGKVFEGYGLSEAPTGTHCNPLMGVNKTGSIGMPLPNMECRV
                     VSLDDGETDLAQGEVGELLLHGPQVMKGYHNMPTETANSLRMGKDGKIWLYTGDIVRM
                     DEDGYFYIVDRKKELIKPGGFQVWPREVEEAIASHPKVLEVGVGGIPDPNRGETVKAW
                     VVLKPEETLSVEELRAYCKERLAPYKVPTHIEFRSELPKTTVGKILRRELIRQHKEAV
                     Q"
     gene            4845..6185
                     /locus_tag="VFQ23_10595"
     CDS             4845..6185
                     /locus_tag="VFQ23_10595"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9907083.1"
                     /translation="MDEWTTCLYALLPHPDQLLVLSSDKDAGKAILPGIRHIGRIWVP
                     DTQTLMPLLEELTGVPVNILRYVAYYRDEEVHQSYCIYLLEPRGTMPDTDLWQPLAKI
                     LADSDIPPALKEGLARWGDEQRLLVTPERRAPWAKTGWHAKVEGWIEDQVSSSGRGNV
                     LRIEPIKSWSISCVLKITTETGVLYFKVPRDLPLFVNEGVVLSRLAEIFPDRVPVPVA
                     LDATKGWMLLDDFGTPAGADASLDYQTNLMQDFARLQIESSRNIERLLSAGCIDRRLE
                     KLLAQIEPLFDDEIVLQTLTPKEREKLKQVPIRLRPLLAELFALPIPVALLHGDLHAG
                     NVVLRGNSFLYFDWTDAAISHPFFDMIHIFMEEDEARKISLQEAYLSIWEENFPKNDV
                     RRAWVLASALYGFYHAVSYQYIVRGIEEIVRSELNFAFYFLRKLLEGIESLDTE"
     gene            complement(6198..7157)
                     /gene="miaA"
                     /locus_tag="VFQ23_10600"
     CDS             complement(6198..7157)
                     /gene="miaA"
                     /locus_tag="VFQ23_10600"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET9907084.1"
                     /translation="MQLPNQKDRADLKQPGRSPLILIVGPTAVGKTEIAIQLAQRLDG
                     EIVSADSRLFYRGMDIGTAKPTQAELATVPHHLIDIANPDEILSLAVFQQKAHEAIAK
                     LHTRNKLPFLVGGTGQYVRAVTEAWSPPEVEPNERLRRELERMKGERGIYWLHEKLKF
                     LDPMSAEKIDARNYRRTIRALEVIFTTGNKFSEQRGQSESPYHLRSIGLMRPRAELYE
                     RVDQRIDAMFAQGFLDEVKSLLAKGYSPTLPTLSAIGYRECIKVISGELSEEQAKAEI
                     RRATRVFVRRQANWFKESDPQIRWFQVTGGVTEEIEKYIRHSL"
     gene            complement(7144..7497)
                     /locus_tag="VFQ23_10605"
     CDS             complement(7144..7497)
                     /locus_tag="VFQ23_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET9907085.1"
                     /translation="MKHNQRIGQWGEDAAATYLIEHGLTIVERNARTPYGEIDIVAKQ
                     GEITVFVEVKALQSSRNFFPEQNVTQRKREHMLAAAESYAAEHKIDHWQIDVISVEGK
                     PASASKITHFENAIT"
     gene            complement(7494..8702)
                     /locus_tag="VFQ23_10610"
     CDS             complement(7494..8702)
                     /locus_tag="VFQ23_10610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9907086.1"
                     /translation="MRIGMMTDTYKPYVSGVTNYIDLNKRLLEDAGHEVYVFTFGELD
                     YQDDDPRVVRSPGMQLGDTGFYLSMRYKAQARKLLQTMDVVHVHHPFLSGRLALRYCR
                     SRQIPIIYTNHTRYDMYAHARVPIIPEELAQSALQAYMPHFCEAVDLVISPSEGMKKV
                     LRDFKVESHIEVVPNGVDLKRFYSASPHDRADFGYKEKDILLVYAGRIAPEKNLEFLL
                     QSFAGVAHVVPNVHLLIFGSGQKDHEEAILPLPEKLGITDRVRFFGMIDYSELPSYLA
                     MCNAFVTASVTEVHPLSVIEAMGAGLPILGIDSPGVGDSVTDGESGLLAHTEDIAAFT
                     AKLTQLCLNNALRKKMGVAAREASKQFDIKRTAKIMLKHYGRLQTNARPVKKRSLDEH
                     LMTILRESLK"
     gene            8807..8998
                     /locus_tag="VFQ23_10615"
     CDS             8807..8998
                     /locus_tag="VFQ23_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9907087.1"
                     /translation="MVKLILREKEFEVKPGMTLLSALKKSDIVPESVIATREGEMILE
                     DEILKDGEVIKLIAVISGG"
     gene            9141..>9614
                     /locus_tag="VFQ23_10620"
     CDS             9141..>9614
                     /locus_tag="VFQ23_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginosuccinate synthase"
                     /protein_id="HET9907088.1"
                     /translation="MKCKKCGEKASVNMRQHKLALCKEHYLEWVPEQTERFIKKYDMF
                     TRDQRILVAVSGGKDSLGLWDILIRLGYQADGLYLGLGIDGGIDYSHESQRLTETFAD
                     QHNLKLHIVDIEKEYGEPIPVLSQISHRGYGKPCAVCGLTKRHEMNRIARDLGYDV"
BASE COUNT         2307 a         2321 c         2447 g         2539 t
ORIGIN      
        1 catctttatg ccttcaatgc ctcccgcggc acctttcact tcaaagccac ggcgacccaa
       61 gataagacgc atcagatcga tcatctcagg ttcatcttca acgcataaaa tacatttcat
      121 tttggcgttc atattgtgga agttaccttt ttttagttta ccataaagtc caaattatga
      181 agataatgac atggaatgtc aacggtttgc gtgcggtgct gggcagaaat ggtttagact
      241 gggcctgggc cgcacaacca gacatacttt gtttacagga agtcaaagca cggcctgagc
      301 aactgaaaga agaataccgt aacttcccag gatacgatgt catttggaac cctgcgctga
      361 aagccggata tagtggggtc gcaacatttc tacgctcggc gtctctcgag actcaacttg
      421 gcatggatgc acctcttttt gatgtagagg gacgcatcat ttccacgctt caccctggct
      481 tccgtttgtt caacgtttac tttcccagcg gacagcgcgg catggatcgg gtgaactata
      541 aattggattt ttatgctcac ctgttggacg tgtgcgaccg actccatgcc aaaggtgaaa
      601 gtgtggtgat agcgggcgat ttcaataccg cacatatgcc cattgactta aagaacgcaa
      661 aacaaaattc caaaacttca ggctttctgc ccgaagagcg cgcctgggtt cagaaaatta
      721 tcgaccatgg ctttgtggat gcctaccgtt ctctttatcc cgatcgtatt caatatacgt
      781 ggtggacgta tcgcctctct gcgcgtgaac gcggcattgg ctggcgcctg gattacttcc
      841 tcgtttcaga gaaactgatg ccgcgggtga gagatgtgat tattcatgac tcggtcgctg
      901 gctccgatca ctgtccggtg gaactggtgc tggagtaaga gtaaggcgat tttcctcatc
      961 cagactgtat tcggctcgaa aaatcatgta ttagagaaat atattaagtt atccagtgga
     1021 ctattcctta ataattcctg ttggtataat tccctttgga attatgatat ggaggcacag
     1081 tgaatcctag aaatcagtca tttgttgtaa ccttcttatt gatcgtggca gccatcgcga
     1141 tggtggtaat ggccgtaaac cgagagtcga caacagaaga atctctgacg atcaatgaag
     1201 ttgccgcaga tattattgcg ggtcgagtgg cccgcgtagt catcgaagat ggtgacagtc
     1261 tgcgtgtcat ttataaaacg ggtggcgaag cccagactag aacagggctc gagtcctaca
     1321 aggaagccaa ttccaccctg gtggaacaat tgctggccct tggcgtgtca gcagaacagc
     1381 tttccgcaga aaacgtgacc atcgaagtca gacctccttc ccagtgggct ggcatcctta
     1441 gtggcgcgct atatattctc cctgtaatct ttatggcagg cgtgctatgg tttattttcc
     1501 gacaggcgca gggctcgaac aatgcagcca tgtcgtttgg taaatcccgc gcccgtatgt
     1561 tcagcggcga gcatccgacc gttacatttc aggatgtggc gggcgtggaa gaatccaagc
     1621 aggaactggc agaagtcgtg gagttcctga aggaacctca gaagttcatt caactcggcg
     1681 cgcgtattcc gaagggcgtt ttgctcgtag gtccccccgg aaccggtaag accctgcttg
     1741 ccaaagcagt atccggagaa gccggggttc ccttcttctc gatctctggt tctgaatttg
     1801 tagaaatgtt tgtgggtgta ggagccagcc gcgttcgaga cttgttcgat caggcaaaac
     1861 gacattcgcc ctgtatcgtc tttgtggatg aaatcgatgc ggtaggtcga cagcgcggtg
     1921 ctggtctggg tggttcacat gatgaacgtg agcagacgct caaccagatg ctagtcgaaa
     1981 tggatggctt cgataccgat acaaacatta tcatcattgc cgctacgaac cgtccggaca
     2041 tcctggatcc cgcgctgctg cgccccggtc gtttcgaccg ccgtgtaacg ctggatcgcc
     2101 cggatatgaa gggccgtgag gcgattttga aagttcatgt caagggcaag ccattggagc
     2161 cagcagttga ccttggctca ctggctcgtg gtacgccagg ctttgttggt gcagatttag
     2221 aaaatctcgt gaacgaaggc gctattttgg cggctcgtcg caataagaag tcgattgggc
     2281 agcaggatct cgaagaggcg attgaacgcg tagtcatggg accagaaaga aagtcccgtt
     2341 tgatctctga tgaagagaag cgaatcatcg cctaccatga agctggtcat gctgtcgtga
     2401 tgaatgccat ccccgaagcc gatcccgttc aaaagatcac cattgtcggt cgcggtcagg
     2461 ctggtggact cacctggtat cgccctgagg atgatcgcat cttaacctca cgtaagaaga
     2521 tgatcgctgc gctggcaggt cttctgggcg gacgcgttgc ggaggaactt gtctttgacg
     2581 atgtcacctc tggcgcatcc agcgatattg agcgcgtcac gcaaatggcc cgcacaatgg
     2641 tcactcgcct gggcatgtcc aatgatatgg gtctcatgac ctacggtcag aaggaggaat
     2701 tgatcttcct gggacgtgag atttctgaac agcgcgatta ttcagaagcc atcgctgaga
     2761 aaattgatgc agaggtccgc aagctggtag atgatgccta taagctgacc aagaagcttc
     2821 tgttgaagta tcgagacaga ctcgacgccg tagcccaaaa gcttctcgaa gtcgaatcac
     2881 tgacccgcga agagtttgag ggcattttcc caccgccatt ccccaagaag agcggaaccc
     2941 ctcaagtgat cgccatgtaa atagatcgcc cgacagtctg accgtcgggc gatttttatt
     3001 ttaaagagag cttcccaatt tgggaggctc tcttgttaca attaacctgc ggtttactga
     3061 acagcttctt tgtgttgccg aatcagttca cgccgaagga tcttccctac ggttgttttt
     3121 ggtaactcac ttctgaattc gatatgggtg ggaaccttat aaggtgcaag gcgctccttg
     3181 cagtacgctc tgagttcttc gaccgacaac gtttcttcag gtttcagcac cacccaggct
     3241 ttgaccgtct cacctcgatt tggatcagga atgccgccaa cgcccacctc gaggaccttt
     3301 ggatgtgacg caatggcttc ctccacctca cgcggccaga cctgaaaacc gcctggtttg
     3361 atcagttcct tcttgcgatc tacgatataa aaatacccgt cctcatccat acggacaatg
     3421 tcgccggtat aaagccatat ttttccgtct ttacccatgc gaagtgaatt tgccgtttca
     3481 gtcggcatgt tgtgatagcc cttcatcact tgcggtccgt gcagcagaag ttctccaacc
     3541 tcaccctgtg ccagatcggt ttcaccatcg tcgaggctga ccactctgca ttccatgttg
     3601 ggtaatggca taccaatcga accggtcttg ttgaccccca ttaatggatt gcagtgggtc
     3661 cctgtggggg cttcggagag tccatagcct tcgaagactt tgccgcctgt tagcttctca
     3721 aactgttctt tcgtttcgcg catcaacggt gcggagccag aaatacaagc cttaatggat
     3781 gaaagatcat atttgccagc cttgacctca ggatgattgt tgatgccatt gtacaaagcg
     3841 ggtacacccg ggaagatggt acagcgatat ttggagatat tttccaatac atccttgaga
     3901 tcgcgcacgt tcgggaccat caccatcgtc gcgccagttg ccatcgcaag attcatgcct
     3961 gctaccatcc catacacatg gaatagtgga atgcccatca ggaagacttc cttaccctct
     4021 tccatattga cgaaccagct gcgcatctgt aatgtgttag ccaggacgtt gcgatgggca
     4081 gccactgcac ctttcgaaac gccagtcgtt ccacctgaat attggaatag ggcggtatct
     4141 tcgggatcaa tcgggatatt gggccgagat tttcccttgt atttcgcaag gagatcctga
     4201 agccaaactt cgccttcctg caaaccgccc tcgattcgaa acccaccttt cttttcacgt
     4261 aacagggtga agaggagcct cattaccggg ggcaaggtct ccttcaagtt ggtcacaatc
     4321 attttcttga tcttcgtgtt gggttgtgcc ttcttgattg tcttatagaa gttggtcatt
     4381 acaaacatca cttcgatgcc tgcgtcactt gcctgatgtt cgatttcagg aggcgtgtaa
     4441 agaggattgg tcgctacgac cgagccacct gccttgagaa tgccataata agccattacg
     4501 aactgcggcg tgtttggcat gaagatgccg acccggtcgc ctctcttcac gcccatatcc
     4561 acaagtgcag cagctactcg atcggtaagt tcattcattt ccttataagt aatgaccgcg
     4621 cctttgaaaa tggtacaggc gcggtctgga tattttcgtg cagactcctc aagcaaatgg
     4681 aataacggtg cactcggaat ttcaacggta tgagggaccc ctttatcgta atgagccagc
     4741 cacggttttt cgttcatggt atctcctctt cactgatttg ttaaagtata ttcaatgcaa
     4801 aatcaaaagt caattgtaaa agggcatttg ctataatttt gggtatggat gaatggacta
     4861 cgtgccttta tgccttactt cctcacccag accagttgct tgttctctcc agcgacaagg
     4921 atgcagggaa agccattctt ccaggcatcc ggcacatcgg gaggatctgg gtcccagaca
     4981 cacagacgtt gatgcctttg ttggaagaat tgacaggcgt gccggtcaat attctccggt
     5041 atgttgccta ttaccgcgat gaggaagtgc accagagcta ttgtatctat ttgctggagc
     5101 cgcgtggaac catgccagat actgacctct ggcagcctct ggcgaagatt cttgctgata
     5161 gcgatattcc gcccgcgctt aaagaggggt tagcgcgctg gggagatgag caacggttgc
     5221 ttgtgacgcc cgagagacgc gcgccctggg cgaaaactgg ctggcacgcc aaggttgaag
     5281 gctggattga agaccaggtc tcgagcagtg ggcgcggcaa tgttttaaga attgaaccga
     5341 ttaagagctg gagtatttcg tgcgtcttga agataacaac ggaaacaggg gttctctatt
     5401 tcaaagtgcc gcgcgatcta cctctcttcg taaacgaagg ggttgtgctt tcccgactgg
     5461 cagagatttt ccctgatcgt gtgccggtgc cggtcgcgct ggatgcgacc aaaggctgga
     5521 tgttactaga cgatttcgga actccagcgg gcgctgatgc ttcgctcgac tatcagacaa
     5581 acctgatgca ggacttcgcg cgcttacaga ttgaatcatc tcgaaatatt gaacggttac
     5641 tcagtgctgg ttgtatcgat cgccggcttg aaaaactgtt ggcacaaatt gaaccattgt
     5701 ttgatgatga aattgttctt cagacgctca cgccgaaaga acgcgaaaag cttaaacaag
     5761 ttccgattcg cctacgccca ttgcttgcag agttgtttgc gttgccaatc cccgttgcac
     5821 ttttgcatgg tgacttgcat gctgggaatg ttgttttgcg aggcaattca tttttatact
     5881 ttgactggac cgatgccgct atcagccatc ccttcttcga catgatccac atctttatgg
     5941 aagaggatga ggctcgaaaa atctccttac aggaggctta cctgagcatc tgggaagaaa
     6001 actttccaaa aaatgatgtc cgccgcgcct gggtccttgc aagcgcattg tatggtttct
     6061 atcatgctgt cagctatcaa tatatcgtga gagggattga agagatcgtt cggtctgagt
     6121 tgaattttgc tttttacttc ctgcgaaaat tgctggaagg aattgagagc ctggatactg
     6181 agtgaaaaag attcacatta caacgagtgg cggatatatt tctcaatctc ttccgtgact
     6241 ccaccggtga cttgaaacca cctgatctga ggatctgatt ccttgaacca atttgcctgc
     6301 ctgcgcacaa atacgcgcgt ggcacgtctt atctcagctt tggcttgttc ctcactaagt
     6361 tcgccgctga tgactttgat acattcgcgg tatccaattg cagacagcgt tggcagggtg
     6421 ggggagtagc ctttggctaa taaacttttc acttcgtcca aaaagccttg tgcaaacatg
     6481 gcgtcaatcc gctgatcgac tctttcatat aactcagctc gcgggcgcat caaaccaatc
     6541 gatctcaagt gataaggaga ttcactttga ccgcgttgct cagagaattt gttgcctgtt
     6601 gtaaaaatta cttcgagggc gcgaatggtt ctccggtaat tacgcgcatc aattttttct
     6661 gcactcatag gatccaggaa ttttagcttt tcgtgtagcc aatagatccc tctttcccct
     6721 ttcattcttt ccaattctct tcgtaatcgc tcattgggtt caacttccgg cggactccat
     6781 gcctccgtga ccgctcgaac atattggccg gtgcctccaa cgaggaacgg caatttattt
     6841 cgcgtatgaa gtttagcaat tgcttcatgg gccttttgtt gaaaaacagc aagactcaaa
     6901 atctcgtctg ggtttgcaat atcgatcaaa tgatggggga cggttgcaag ttctgcctga
     6961 gtcggttttg ccgtgccgat atccataccc cggtaaaaga gacgcgaatc ggccgacaca
     7021 atttcgccat ccaatcgttg tgccaattga atagcgattt ctgttttgcc gaccgctgtt
     7081 ggtccaacaa tgagaatgag gggagatctg ccgggttgct taagatctgc ccggtctttt
     7141 tgattaggta attgcattct cgaaatgagt gatctttgaa gcagatgcgg gctttccctc
     7201 cactgaaatc acatcaattt gccaatgatc gattttatgt tctgccgcgt aggactctgc
     7261 tgccgcgagc atgtgttcgc gtttgcgctg cgtgacgttt tgctccggaa agaaatttcg
     7321 gctggattga agagctttta cttcaacaaa aacggtaatc tcaccttgtt tcgctacgat
     7381 atcaatttcc ccgtaaggcg ttctggcatt gcgttccacg atggtcaggc cgtgttcaat
     7441 caggtatgtc gcagcggcgt cttcgcccca ttggccaatg cgctgattat gtttcacttg
     7501 agggattctc tcaggatggt catcaggtgt tcatccaggc tgcgcttctt aaccggtctg
     7561 gcgttagtct ggagtcgacc ataatgctta agcatgattt ttgcagttcg tttgatatcg
     7621 aattgcttgg atgcttcacg cgcggccacc cccatttttt ttcgcagggc attgttgagg
     7681 cacagttggg taagcttggc ggtgaatgca gcgatatctt ctgtgtgagc aaggagaccc
     7741 gattctccat cggtcactga gtcgccgacg ccaggggagt cgatgcccag aattggaagt
     7801 ccggctccca tggcttcaat caccgaaagc ggatggactt ctgtgaccga ggcggtcaca
     7861 aatgcattac acatggccag gtaagatggc aattcactgt aatcgatcat tccaaaaaat
     7921 cgaactcgat cagtgatgcc aagtttttca gggagtggca ggattgcttc ttcgtggtct
     7981 ttttgcccac tgccgaaaat caaaaggtga acgtttggaa ctacatgggc gacgcccgca
     8041 aaggactgaa gtaggaattc cagatttttc tcgggtgcga tgcggcctgc atacacaagc
     8101 agaatatctt tctctttgta gccgaaatct gcgcggtcat gaggactggc ggaataaaat
     8161 ctttttaagt ccacgccatt gggcacgact tcaatgtggc tctccacttt gaaatctcgc
     8221 agaacctttt tcataccttc cgatggcgaa atcaccagat ctaccgcttc acaaaagtgc
     8281 ggcatatacg cttgaagtgc gctctgggct aattcttcgg gaatgatcgg gacacgggcg
     8341 tgagcgtaca tatcatagcg agtatggtta gtatagataa tcggaatctg ccggctacgg
     8401 caatagcgta aggcgaggcg cccgctcaga aatggatgat gaacgtgaac cacatccatg
     8461 gtttggagga gcttgcgtgc ctgggcttta tagcgcatcg acagataaaa gcccgtatcg
     8521 cccaattgca ttcccggtga tcggaccacg cgcgggtcat cgtcctggta gtcaagctct
     8581 ccaaaggtaa agacgtaaac ctcatgtccc gcatcttcca gcagacgctt gttcaggtcg
     8641 atgtaatttg taacgccgct cacgtagggc ttgtaggtgt ctgtcatcat tccaatacgc
     8701 atacgtgaat cgtaaaggtt tgcagcacac ttgtcaagcg atttctctac gtgatatccc
     8761 gactatgacc ggatcgctat ggtagaatga ttcgtgagga tcaagcatgg ttaagttgat
     8821 tttgcgtgaa aaagaattcg aagtaaaacc aggtatgacc ctgctctcag cgctgaagaa
     8881 aagtgacatc gtacccgagt ctgtgatcgc aacaagagag ggagagatga ttctggagga
     8941 tgaaattttg aaagatggag aggtgatcaa gctgatcgct gtgatttctg gaggctaagg
     9001 tcattgcgag gagggttgag gcaagctagt gcctaaatcc ctacgaagca atctcaagta
     9061 acctctaaat aaaagcttgg tggtacgacg agattgccac gtcgccgctt cgcggctcct
     9121 cgcaacaaca tgaaaaaact atgaagtgta aaaaatgcgg tgagaaggcc tcggtcaaca
     9181 tgcgtcaaca taagctggcg ctatgcaaag aacactatct cgaatgggtt cccgagcaaa
     9241 ccgagcgctt tattaaaaaa tatgacatgt tcacacgcga ccagaggatt ctcgtcgcgg
     9301 tatcgggcgg caaagattcg ctgggattgt gggatattct tatccggctg ggctatcaag
     9361 ccgatgggct ttatcttggc ttaggtattg acggtgggat agattattct catgagtcgc
     9421 aacggctcac agaaacgttc gcggatcagc ataatctgaa attgcacatt gtcgatatcg
     9481 aaaaggagta tggggaacca atccctgtgc tttcgcagat ctcccatcgc ggctatggca
     9541 aaccttgcgc ggtctgcgga ttaacaaagc gtcacgagat gaatcggatc gcgcgggacc
     9601 ttgggtatga cgtt
//
LOCUS       DASTJP010000356         4029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_21515, whole genome shotgun sequence.
ACCESSION   DASTJP010000356 DASTJP010000000
VERSION     DASTJP010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21515"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..676)
                     /locus_tag="VFQ23_10625"
     CDS             complement(290..676)
                     /locus_tag="VFQ23_10625"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HET9907089.1"
                     /translation="MTYTQLAYLHLGTVLPAFVIGTFQLLTRKGTRSHKLLGRIFMLL
                     MLATGLITLLMPAKVGPQFLNHFGFIHIFSFFAVFGVPLSYLAARRGFIRAHRFTMVS
                     LYVGGILVAGAFAFSPGRMLHEWLFG"
     gene            complement(797..1333)
                     /locus_tag="VFQ23_10630"
     CDS             complement(797..1333)
                     /locus_tag="VFQ23_10630"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9907090.1"
                     /translation="MKPWKTKSRQTVLSQRPWLVVENHTVELPHGRLIPNWPWIITPD
                     YVNVVAVTEDKRFICFRQVKYGVDGTTLGIVGGFVEEGEEPMIAARRELLEETGYEST
                     DWTPLGSYRVDPNRGIAMGHFYLVRQARYVTPPNADDLEEQEILLLTRLEVETALARE
                     EFKVLAWAACLALALRQL"
     gene            complement(1482..2045)
                     /locus_tag="VFQ23_10635"
     CDS             complement(1482..2045)
                     /locus_tag="VFQ23_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907091.1"
                     /translation="MKKLVCWELYILSAALLAACAMATPERAEEMLKPGDKLGNMTVE
                     EHAMSTKYPDIWDYCAYQPEEKEPGTQTIDCDVPLISRMEIGFGWGAKDVAILDSNWS
                     AMAWELHIDGYQINLDEFEQWSENGRPDLGVRQAARGWILDLKNLSPGKHTLRYLWTS
                     EISIDDGFDIYTPGTREYIVNSTVLEK"
     gene            complement(2092..2388)
                     /locus_tag="VFQ23_10640"
     CDS             complement(2092..2388)
                     /locus_tag="VFQ23_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907092.1"
                     /translation="MTMYVRLITFHLEPGMYKTAEKLADRFAPILRARRGLITAIFFV
                     NDAAGEYGALTIWESKEDVEVEATIVIPRLQDALAGISAAPPLIRLYEVYEPQT"
     gene            complement(2397..2945)
                     /locus_tag="VFQ23_10645"
     CDS             complement(2397..2945)
                     /locus_tag="VFQ23_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907093.1"
                     /translation="MKIFFPLRFNLLMIAVVIACQSTEQFPAKVMLKPGDRIHGMTLT
                     NGTAEAVPLWAFCSSVQKNEQMLTTECHIPLSTRLAIGQVFFPTDKALTELDWSEFTW
                     ELSIDGRSLDLDAFGTYDYVSPAISHPPCPIREVFIKSTGWDIVLSELKPGTYLFRGQ
                     AQSETESYTWIINLIVEPSEAL"
     gene            3153..>4029
                     /locus_tag="VFQ23_10650"
     CDS             3153..>4029
                     /locus_tag="VFQ23_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9907094.1"
                     /translation="MAEVSFGEWVKRRRRARGLTQEQLARQLSCSTSALRKIEAEERR
                     PSEQMVERLAEVFSIPSNEQIAFLHFARGNWRFAPAEVMIDAPWRESNASPRSNLPTP
                     LTSFIGREKEQAVILKLVSKYRLVTLVGSGGVGKTRLSLKIGEQVLEEYVNGVWFVDL
                     APILDPLLVPRITAIAIGLRDEPQRPVIDMLSDYLREKKMLIIIDNCEHLLDVCAQLT
                     DTLLKNCPNLKILATSREALGILGEAVYHVPSLELPDMQQLLEKFRGYESVRLFEERA
                     QLAQVDFSLTLENASS"
BASE COUNT         1006 a         1022 c          988 g         1013 t
ORIGIN      
        1 acggcataag ggttgggacg cagctgccga taggtcttcc tgaacccgag agtttcgtag
       61 aatgctatgg attcatcgag ttcgcgacag ggtagacacg gataagtacg ttcattcgcc
      121 ataaacagac tccttatata cttcttgccg ggttattttc attatagaag gttggtgaac
      181 agtgggaggg tttggagtca ggacgggata aacaagccgc gttctggtcg cggcttgtgt
      241 gagcgaaaaa ctctacatgt gctggaaacc tgcttacgaa ctggaatatc tatccaaaca
      301 gccattcatg caacatacgc cccggtgaga aggcgaacgc tcccgcgacc aggatgccgc
      361 ccacatacag cgagaccatc gtaaaccggt gggctcgaat aaaacccctg cgcgcggcaa
      421 ggtaggagag aggaacgcca aaaactgcga agaaactgaa aatatggatg aacccgaagt
      481 ggttcaaaaa ctggggtcct accttcgctg gcatgaggag cgtgatcaaa ccggtcgcca
      541 gcatgagcaa catgaagatc cttccaagca gtttatgact tcgagtgcct ttccgggtga
      601 gcaattgaaa agtaccaatg acaaacgccg gaaggacggt ccccagatgc aagtaggcaa
      661 gttgtgtgta cgtcatttcc atactcctat ataagctatg atcattaact tgaggttttg
      721 ggtgtttata taggcgctaa aaagagcatg cagctatgcg ggctcgtaaa ggattcctgc
      781 agcatttatc ctagcactag agttgacgta gcgcaagcgc gaggcatgcc gcccaggcca
      841 acaccttgaa ttcttcccga gccaaagcag tttctacttc caaacgtgtc agcagcagga
      901 tttcctgttc ttccaagtcg tccgcgtttg gtggcgtcac ataccgcgcc tgacggacca
      961 ggtaaaagtg tcccatggcg atcccccggt tgggatctac gcggtaactt cccaacggag
     1021 tccagtccgt tgattcgtag cctgtctcct ccagcagctc gcggcgggcc gctatcatgg
     1081 gctcctcccc ttcttccaca aaccctccca cgatgcccag ggttgtacca tccacaccat
     1141 acttgacctg ccggaagcag atgaaacgct tatcctcagt cactgccact acattgacgt
     1201 aatccggcgt aatgatccac ggccagtttg gaatgagacg tccatgcggc aattccaccg
     1261 tatgattttc cacaacgagc catgggcgct gagataggac agtttgacgg cttttggtct
     1321 tccagggttt cataatcatc tcctggaata ggataatacc aaacgaataa gccgcatctc
     1381 accggtgctt tgatcttatt gcttgttgga ttactaacct gtcctcattg aaataagact
     1441 gctccttatt caatcggcat cgtacgctgt caggttcgtt cttacttttc cagaactgtg
     1501 gagtttacaa tgtattcacg cgtacctgga gtgtagatat cgaagccatc gtcaatcgat
     1561 atctccgatg tccataaata acggagagtg tgcttgccgg gagaaaggtt cttcagatcc
     1621 aggatccaac ctcgtgcagc ctggcgcacg ccgagatcgg gcctgccatt ctcgctccac
     1681 tgttcaaact catcgaggtt aatctgataa ccatcaatgt gcaattccca tgccatggca
     1741 ctccagttcg agtcaagaat ggcgacgtct ttggcgcccc atccaaagcc gatttccatc
     1801 cttgaaatca gtgggacatc acaatcaatc gtttgagtgc cgggttcttt ttcttcgggt
     1861 tgataggcac aatagtccca aatatccgga tattttgtgc tcattgcatg ctcttccacc
     1921 gtcatattgc caagtttgtc gcccggtttg agcatctcct ccgcacgttc tggcgtagcc
     1981 attgcacagg ccgccaatag ggcggcagac aatatataca gttcccaaca gacaagcttt
     2041 ttcatggttg ttctcctcgt aatcgaattg gaaaatgatc aggcgtgcct ttcatgtttg
     2101 aggctcgtag acctcataca gacgaattaa gggcggcgca gcactgatac ccgcaagtgc
     2161 atcctgtaaa cgtggaatca caatagttgc ctctacctca acatcctctt tggactccca
     2221 aatcgtcagc gcgccgtatt ctcccgccgc gtcattcacg aagaagatcg cagtaatcag
     2281 cccgcggcgc gccctaagga tcggcgcgaa gcggtctgcc agcttctccg ctgtcttgta
     2341 catccctggt tccagatgga aggtaatcaa gcggacgtac atggtcatgt atcctgctac
     2401 aacgcctctg acggttcaac aataagattt atgatccagg tgtaagattc ggtttcagac
     2461 tgcgcctggc cgcgaaataa atatgtgccg ggtttcaatt cgctcaaaac gatatcccaa
     2521 cccgtagact tgataaacac ctcccggatg gggcaaggag gatgtgatat ggctggcgag
     2581 acataatcgt acgtaccaaa agcatccaaa tctaaacttc gaccatcaat ggatagttcc
     2641 catgtgaact ctgaccagtc caattcagtc agtgccttgt ccgttggaaa gaaaacctgg
     2701 ccgatcgcca gcctagttga caatggtata tggcactcgg tggtaagcat ctgctcgttc
     2761 ttttgaacgg aggagcagaa agcccataat ggcacggcct ctgcggtacc attcgtcaga
     2821 gtcatgccat ggatacggtc gcctggctta agcatgacct tggcaggaaa ttgttctgtt
     2881 gactgacagg cgatcacaac agcgatcatc aataggttga acctcaatgg gaagaaaatt
     2941 ttcatttctg gctccttgtt tttcaccgat ttcagatcac actccttgtg cactttgaaa
     3001 acctgtcctg tattcctaga gtatgtaaag gtaggagatg gttcggaaca gcgatggtat
     3061 acttcgtttg acaattagac tctacaacgt atccaataca cgtgggtgtc actcagcgcc
     3121 accaaaagtg acacagttta tggagaaatg cgttggctga ggtatctttt ggagaatggg
     3181 taaagcgccg acgtagagca cgaggcttga cgcaggaaca acttgcccgg cagctcagtt
     3241 gctcgacgag cgccttgcgc aagatcgaag cggaggaacg ccgtccatcg gagcagatgg
     3301 ttgagcggct ggcggaagta ttcagtattc cctcaaatga acaaatagcg tttctacatt
     3361 tcgcacgtgg caattggcga tttgctccgg ctgaggtgat gatagatgct ccctggcgtg
     3421 aatcaaacgc gtctccgcgc tcgaatttac cgactcctct cacaagtttt attggccgtg
     3481 aaaaagagca ggcagtaatt ctcaaactag tcagcaaata tcgtcttgtc acgttagtcg
     3541 gttcaggcgg tgtcggcaaa acgcgtttat ctttgaagat cggcgaacaa gtgttagaag
     3601 agtatgtaaa tggcgtatgg tttgtagacc tcgctcctat tcttgaccct ctgctggttc
     3661 cacgtatcac tgcgattgct attggcctgc gtgatgagcc gcaacgcccc gtcatcgata
     3721 tgctttccga ttatctacgt gagaagaaaa tgctcatcat tatagacaac tgtgagcatt
     3781 tgttggacgt ttgtgcgcag ttaaccgata cgttattgaa gaattgccct aacctgaaaa
     3841 ttcttgccac cagccgcgaa gcgttaggca ttctggggga agccgtgtat cacgttccct
     3901 cccttgagtt gcctgatatg cagcagttgc ttgaaaaatt tagaggctac gaatccgtcc
     3961 gcttgtttga agagcgcgct caactcgctc aggtagattt ttcgctaacc ctggagaatg
     4021 cttcctcta
//
LOCUS       DASTJP010000357         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_870104, whole genome shotgun sequence.
ACCESSION   DASTJP010000357 DASTJP010000000
VERSION     DASTJP010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870104"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFQ23_10655"
     CDS             <1..687
                     /locus_tag="VFQ23_10655"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9907095.1"
                     /translation="EDPIQARTLNTDLPRELATACVKRNIRFAHISTDAVFDGTKEGF
                     YMEEDEPNPQGIYSQSKLEGEHAVQNANPQAIIARVNFFGWSLTNRRSLGEFFVNNLS
                     AGKEVNGFTDVIFCPMLVNHTARLLLKMVEKNLSGLYHVVGAQAMSKYQFGLEIARKF
                     GLRESLISPKSVEESELTAKRSHNLRLSIHKLSTALGNPIHAFSTGLDEFYTQYQQGY
                     PQKIRGYQQP"
     gene            756..1106
                     /locus_tag="VFQ23_10660"
     CDS             756..1106
                     /locus_tag="VFQ23_10660"
                     /inference="COORDINATES: protein motif:HMM:NF025445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HET9907096.1"
                     /translation="MKNLLKLEELFLFGLALFLFSQLDYGWGWYALLFLAPDLSMVGY
                     LANPRLGSWTYNLIHHKALAVALYVLGYLLSIPWLMFAGTLLLGHSSFDRVFGYGLKY
                     PDSFQNTHLGKIGR"
     gene            1206..2273
                     /locus_tag="VFQ23_10665"
     CDS             1206..2273
                     /locus_tag="VFQ23_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HET9907097.1"
                     /translation="MEIKFGNRTIGLNHPTYFIADIAANHDGDLDRAKLLIRLAKEAG
                     ADAAKFQNFDAPKIVSDYGFSHMNSQVSHQATWKKSVTEVYSAASIPFDWSMTLVEEC
                     QDVGIDYFSSPYDFNAIDFLDQYVPVYKAGSGEIDWIEALERMASKGKPFFVATGAST
                     IGEVQKAVHAILKINKQLVLMQCNTNYTASPDNYDHLHLNVLKTYASMFPDVILGLSD
                     HTHAVAPVVGAVTLGARVIERHFTDSNNREGPDHKFAMDPEKWARMVEETRLLERSLG
                     SSDKFIAGNEQETAVVQRRCLRAARDIKAGEVFTRDMIDVLRPATIGAIKPDQIENVI
                     GTKALHDFKYGQELRWTDLGT"
BASE COUNT          648 a          640 c          607 g          577 t
ORIGIN      
        1 gaggacccaa tccaagcaag aacattaaat acagatttgc cccgggaact ggcaactgcc
       61 tgcgtaaaga gaaacattcg cttcgcgcat atttccacag atgccgtctt cgatggcaca
      121 aaagaaggct tctatatgga agaggatgaa ccaaatccac agggaatcta ttcgcagtcc
      181 aaacttgaag gtgagcacgc tgttcagaat gctaatccac aggcgatcat agcccgtgtc
      241 aatttttttg gatggagtct caccaaccgc cgcagcctcg gagagttttt cgtcaacaac
      301 ctgagcgcag ggaaagaggt caacggattc acagatgtca tcttttgccc aatgctcgtc
      361 aaccatacgg cccgacttct tcttaaaatg gtagagaaaa acttgagcgg cttatatcat
      421 gtagttggcg cccaggcgat gagcaagtat cagttcgggt tggagatcgc gcgaaagttc
      481 gggctaaggg aaagcctcat ttcaccgaaa tccgttgaag aatccgagtt gaccgccaaa
      541 cgttcccaca atttacggct atctatccac aagttatcca cagcgctggg gaatccaatc
      601 cacgcgtttt ccaccggttt ggacgagttt tatacacagt accagcaggg ttatccacag
      661 aaaatcaggg gttatcaaca accgtagggc gggttgacaa tccgtctttc acatattgcc
      721 cacagaaaac gacgagttat ccacaggatt tccacatgaa aaaccttctc aaacttgaag
      781 aactattttt gtttggactt gcactctttc tcttttcaca attggactat ggatggggct
      841 ggtacgcact gttgtttttg gcgccggacc tgagcatggt cggctatctg gcgaatcccc
      901 gcctcggctc ctggacctat aacctgatcc accacaaagc actcgccgtc gcgctttatg
      961 ttttgggcta cctgctctcc atcccctggc tgatgttcgc agggactctc ctgctcggcc
     1021 actccagctt cgaccgcgtc ttcggctacg gcttgaaata ccccgattcg tttcagaata
     1081 cgcatttggg gaagattgga agatagaatg ataccaataa ggtgacagtc acttctgcga
     1141 agcatccctg aagcgcacgc aaaggggaca tagtgactgt cacctataga taattggagg
     1201 acaacatgga aatcaaattc ggcaatcgaa ccatcggctt gaatcatcca acttacttca
     1261 tcgcagatat cgccgccaat cacgacggcg acctggatcg ggcaaaactg ctcatccgcc
     1321 tggcaaaaga agcgggcgcc gacgccgcca aattccagaa ctttgacgcg ccgaagatcg
     1381 tctcggacta tggcttctcg catatgaact cgcaagtcag ccatcaagcc acgtggaaga
     1441 aatccgtgac cgaagtctac agcgccgcgt cgattccctt cgattggtcg atgacgttag
     1501 tcgaggaatg tcaggatgtg ggcattgatt acttctcctc gccgtatgac ttcaacgcga
     1561 ttgatttcct cgaccaatat gtgcctgttt ataaggctgg ctctggcgaa attgactgga
     1621 tcgaagcgct ggagcgaatg gcaagcaaag gcaaaccgtt cttcgttgcg accggtgcat
     1681 ccacgatcgg tgaggtgcag aaagctgtcc atgcgatttt gaagatcaac aaacagttgg
     1741 ttctgatgca gtgcaacacg aattacactg ccagccctga taactacgat cacctgcatt
     1801 tgaatgtact aaaaacctat gcaagcatgt ttccagatgt cattctgggg ctatctgacc
     1861 atacgcacgc ggtcgccccg gtggtcggcg ctgtgaccct gggcgcgcgc gtcatcgagc
     1921 ggcatttcac ggatagcaac aatcgtgaag gtccggacca caagttcgcg atggatcccg
     1981 agaaatgggc gcggatggtc gaagagacgc gcctgctcga acgctcgctt ggctcctctg
     2041 ataagttcat cgctgggaat gagcaggaaa ctgctgtggt acaacgtcgc tgcctgcgcg
     2101 ccgcgcggga tatcaaggcc ggcgaggtct tcacccgcga catgatcgat gtgcttcgtc
     2161 ccgccacaat cggcgctatc aagcctgacc aaattgaaaa tgtcatcggc acgaaagcgt
     2221 tgcatgattt caaatatggt caagaattaa gatggactga cctcggcacg tgaaaacatt
     2281 agccacagag tcacggagac tctgagatta aaaaactccg cgactctgtg tctctgtggc
     2341 aaagttatga gaattatcta ctttagtaaa aactatactc ctcacgatta tcgctttctc
     2401 gcttcattat cgaagacaaa acatgagatt tattatctga agctggaggc gaacacgcgc
     2461 caagtggaag at
//
LOCUS       DASTJP010000358         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_852556, whole genome shotgun sequence.
ACCESSION   DASTJP010000358 DASTJP010000000
VERSION     DASTJP010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852556"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VFQ23_10670"
     CDS             <1..1119
                     /locus_tag="VFQ23_10670"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HET9907098.1"
                     /translation="GNQVWLDSNANGLHEVWERGVGGICVNLYDASLSLLQQTTTDSN
                     GYYGFNVERGTYFVEFLKPAGMEFTQRDAEADEQDSDVDQAIGRSELLEFSTSQLDLD
                     AGLLAHDSLPAASELAESKIGPVRSGRLVYADVQAFFPDSCLIYAFASDEVLAELPKC
                     GFVTHDVQGGGYMFGIQEMISLAESRKNPNAIVNYTSNVYSEMPPEGGIPASRLHVYM
                     AWLNQSAWAYDPLAQSWWRYVDDASEQNAGVVHPETDRLTRRQLLFENVIVLFAYHDV
                     ISPTNLDIHLEHDWVGNATLFRDGKKYDIRWSTRATTEEVETGRRKPIQFYYPDDTTL
                     FPLKPGHTWVLVVTPETAVTEQSSGEWLLQFSQPPGSK"
     gene            1142..>2562
                     /locus_tag="VFQ23_10675"
     CDS             1142..>2562
                     /locus_tag="VFQ23_10675"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HET9907099.1"
                     /translation="MFRRTFSICLYFLVLTGCGGNLVIESTPTHTMTPLLTVSPTITT
                     TPAPTFTPQPYLAPISYGPDLEDFPSSVNPLTGQEVADPSLLNLPAVLVSISNMPVTT
                     RPQAGPGFVPWIFEIFIGEGTTRFMGVFYGDNLRHVPNVSGGCSVREEIIRPDGKWIG
                     NRVWLDENENGQQDAWELGVGGVCVRLLDAISREVIAETSTDSNGYYAFDIPNTEAII
                     QFAKSDSYTFTQPNVDDEDRDSDADVNTGETHSLELNSTESFWDAGLILVNEPVPTTS
                     PVVTGTPPNWYIPSEAYVGPIRSGRLTYNQIGGMFPNSCLVYASAAPDIGERLDACEI
                     VYGVDRTTPNSALLTLKRVRELAKENLNPRQPVNYSGNAFSDEVPAGGLPADTINVYY
                     HLYSQSGWQYDPISRTYLRFTDRADTTGELFPATERLTGRQQAFENVIVVFAEHGIFR
                     PGQFEVNLSQGQKQLAYLFRDGQA"
BASE COUNT          621 a          654 c          664 g          623 t
ORIGIN      
        1 ggaaaccagg tctggttgga ttcaaatgcc aatggacttc atgaagtgtg ggagcgcggc
       61 gttggaggta tttgtgtcaa cctttatgac gcgagtctat ccctgttgca acaaacgacc
      121 accgatagta atgggtatta tggattcaat gtagaacggg gaacatactt tgttgagttt
      181 ctgaaaccag ccggaatgga atttacccaa agagatgccg aagcggacga acaggatagc
      241 gatgtggatc aggcaatagg gcggagcgag ctgttggaat tttccacttc acagcttgat
      301 ctggatgccg gacttcttgc ccacgacagt cttcccgctg cttcggagtt agctgaatcg
      361 aaaattggac cggttcgttc cggacgcctg gtctatgccg atgttcaggc cttctttccg
      421 gactcgtgcc tgatctacgc ctttgcttcg gatgaagtgt tggcagaact acccaaatgt
      481 ggatttgtca cccatgatgt gcagggcggc gggtatatgt tcgggattca ggaaatgatc
      541 tcactggcag agtcacgaaa aaatccgaat gcaatcgtga actatacaag caatgtctat
      601 tcagaaatgc cgcctgaggg tggaatccct gcgtcgcgtc ttcatgtata tatggcctgg
      661 ctcaatcaat cggcatgggc gtatgatcca ctggctcaat cctggtggcg gtatgtggac
      721 gatgcaagcg aacaaaacgc cggggttgtt caccccgaga ctgatcggct gacacgccgt
      781 cagttgcttt ttgagaacgt gattgttctg tttgcctatc atgatgtgat ctccccgacg
      841 aatctagaca ttcacctgga acatgattgg gtcggcaatg ccacattatt ccgtgatgga
      901 aaaaaatacg acattcgctg gagcacgcgc gcgacgacgg aagaagtgga aacgggtcgc
      961 cgcaagccga tccaatttta ttatcccgat gacactacac tcttcccact taaaccaggt
     1021 catacctggg tgctggtcgt cacgcctgaa actgccgtga cagaacaatc atcaggtgaa
     1081 tggttgttgc aattcagcca accgccaggc tcgaagtaaa ccagaagtag agatttgaga
     1141 attgtttcgt cgaacctttt cgatttgtct ttatttcctg gttctcactg gctgtggtgg
     1201 taatcttgtc attgaatcga ctcccacaca tacgatgaca cccctactca ccgttagtcc
     1261 cacaataacg accacgcctg cgccaacttt caccccacaa ccttacctgg ccccaatctc
     1321 atacgggcct gatcttgagg attttccatc gagtgtcaat ccactgacag gtcaggaagt
     1381 ggcagatcca tcactgctca atttgccggc tgtgctggtc tcgatcagta atatgcccgt
     1441 gaccacgcgt ccgcaggcgg gaccaggctt tgttccgtgg atctttgaga tcttcatcgg
     1501 tgaaggcacc acccgattca tgggcgtctt ctatggggac aacctgcgac atgtacccaa
     1561 tgtcagcggc ggatgtagtg tgcgcgaaga gatcatccgc cctgacggaa agtggatcgg
     1621 caatcgcgtc tggcttgatg aaaacgaaaa cggtcagcag gatgcctggg aactaggtgt
     1681 tggcggagtg tgcgtcaggt tgctggacgc aatcagccgc gaggtgatcg cggaaacctc
     1741 gaccgattca aatggttact acgcgtttga tattccgaat acggaggcaa taatccaatt
     1801 tgccaaatct gattcttata ctttcacaca gccgaatgtc gatgacgaag accgtgatag
     1861 cgatgcagac gtaaatactg gggaaacgca ctctttagag ctgaactcca ccgaatcatt
     1921 ctgggatgcc gggctgatcc tggtgaatga gcccgtgcct acaaccagcc cagtcgttac
     1981 cggaacaccg cccaattggt atattccctc agaagcttat gttggtccca tccgctccgg
     2041 caggctcacc tacaaccaga tcggcgggat gttccccaat agttgtcttg tgtatgccag
     2101 cgcggcaccc gatatcggcg agcggcttga tgcctgtgaa attgtctatg gtgtggatcg
     2161 gacgacaccc aatagcgcct tactgaccct taagcgtgta cgcgaactgg cgaaagaaaa
     2221 cttgaatccc aggcaacctg tcaactactc aggcaatgcg ttttctgatg aagtccctgc
     2281 gggcggactg cctgctgata cgatcaacgt ttattatcat ttgtacagcc agtctggctg
     2341 gcagtatgac ccgatttcac ggacctatct gcgtttcaca gatcgcgcgg ataccaccgg
     2401 cgagttattc cccgcgaccg aacgactgac tggcagacaa caagcctttg aaaatgtgat
     2461 cgttgtcttt gccgaacatg gaattttcag acccggccaa tttgaagtca acctgagcca
     2521 gggacaaaaa cagcttgcct atctgttccg cgatggacag gc
//
LOCUS       DASTJP010000359         4480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_450477, whole genome shotgun sequence.
ACCESSION   DASTJP010000359 DASTJP010000000
VERSION     DASTJP010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450477"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..549)
                     /locus_tag="VFQ23_10680"
     CDS             complement(157..549)
                     /locus_tag="VFQ23_10680"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HET9907100.1"
                     /translation="MNTKPRFSEEIIATLHKDRIMGIRAGGNSDHRVIGIWVVVVNGR
                     VFVRSYTMKQGGWWRTFLEDPYGEIFVSKRKRGIKVRAVQTRSEKVKDAVSAAYKEKY
                     NTRGSVGYVEEMSKKPLRDTTTELVAIK"
     gene            complement(576..902)
                     /locus_tag="VFQ23_10685"
     CDS             complement(576..902)
                     /locus_tag="VFQ23_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907101.1"
                     /translation="MILTVLITIIIEGTVVLGYSFWHKKPFRPILYTSIVANLITQSL
                     LWVLLNLFFQHYLISLFLAEILIWIIESAMLYYVPANKLLLLEATFLSLLMNLASFGL
                     GWFLPI"
     gene            complement(899..1426)
                     /locus_tag="VFQ23_10690"
     CDS             complement(899..1426)
                     /locus_tag="VFQ23_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907102.1"
                     /translation="MASKLIKPFVVLLLLQAFLPIQVAFADTGPKPTMEFQIRFESGV
                     EESGIVSGILFQCNESDCSDATPLEEVGPQRLYCEPQSCRAIGYGFAPYSLLELEFSD
                     GVTRRSNIFEQAGFDSRYTVYVRPEDLMVETKFSLVPSLSLGILEVLACICALIGLGL
                     IAGLGIFLIRRSKTT"
     gene            complement(1459..1809)
                     /locus_tag="VFQ23_10695"
     CDS             complement(1459..1809)
                     /locus_tag="VFQ23_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907103.1"
                     /translation="MKKLRFFVLSVVFLVLVAGCGPGTNVQVTTPNSTMQLSAPGPNP
                     LMNQPDSTGRIARAGAGLWHGIISPATLLVSFFNSDVRMYQVHNAGSEYDLGFLVGVA
                     IVISIISLLIRMRR"
     gene            complement(1984..3108)
                     /locus_tag="VFQ23_10700"
     CDS             complement(1984..3108)
                     /locus_tag="VFQ23_10700"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HET9907104.1"
                     /translation="MNNLYEALEVCLQEIELGTDIETSLLRYPDIADELRPILETSVH
                     AKGLVVSVPSNDMLQRNRAKLLQRAAELRESQLKSAQTPTWFISLRRAAIALAIIAIA
                     FVSGTGLVRASSTALPGDNLYSVKRTGENLFLLLTFDSQRRDSLEVEYETERLSELQE
                     LIAIGRSVEVDFVGIVTQQNGNDLLVAGVPVRITSQTELHDGAVSLGDAVRVFGITQS
                     DKSVLAERIDQLPEGANLPDAGNSGNGSGSGSNSGSGSGDSGNQNDSSGPGNGNDNSN
                     ENNGEPGNGNDNINDSNSGPGGGNDNSNDNNSGPGGSDNSNSDSSGSESGTENQNNSG
                     SGSSRDQSNDNGSEDNSGSGGGNDNSNEDNSGPGGGGGDD"
     gene            complement(3101..3754)
                     /locus_tag="VFQ23_10705"
     CDS             complement(3101..3754)
                     /locus_tag="VFQ23_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9907105.1"
                     /translation="MLGAKVEIIEDVILNAQAAGIYLTAILAWGAAQQPMSTYNEQQA
                     LHGLRKLDSSAIGAIYDQYFAEVYRYIRYRIADDRIAEDLASDVFVRLLEAAQKNQGP
                     QTSLKGWLIATASNTVNDYLRQKYRRPVEQLSDSMPDGQMSVLAEVDLREQTQLVQRA
                     YAQLTHEQQDVLALRFGQGYSLEETAVHMKKNVNAIKALQFRALASLQRQIGEMSHE"
     gene            complement(3764..>4480)
                     /locus_tag="VFQ23_10710"
     CDS             complement(3764..>4480)
                     /locus_tag="VFQ23_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907106.1"
                     /translation="LIAFASGTLALGLAGRKMDVRTAFAFLTSLLFGLMLFQARRFVE
                     YFPPFALIFAAFAWAPLFDVRLGSVISTDSAALQRRFSWRDRIPVILLSAAVAVSIAR
                     SIPQTREAINSSKSYDLYAGASAWLEDNTPPNSRVFQTDWDDFPRLFFYNTHNTYLIG
                     LDPTYMQLYDPDLYELWVEITQGDVENPSQVIASRFGSQYIHTDLNHGDFLQVAAEDP
                     GLKEVYRDDQSVLFEVRALP"
BASE COUNT         1158 a         1152 c         1050 g         1120 t
ORIGIN      
        1 gtggcttgcg tgccaaccgg cgcgaaaaca ggtgtgagga ggtctccatg aggcgtagcg
       61 aagatgcctg tgcgggcgcg gttatttctg gccgttattt gaaattgaaa catcggggga
      121 attatacgat actcgttatt cgagacagga aatcttttac tttatggcca ccaactccgt
      181 tgtggtatcc cttagcggct ttttagacat ttcctccaca tacccaaccg accctcgtgt
      241 gttgtatttc tccttatagg ctgcgctgac tgcgtctttt actttctcac ttctcgtctg
      301 cactgcacgg actttgattc cccgctttcg cttggaaaca aagatctccc catacgggtc
      361 ttcgagaaac gtccgccacc agcctccctg tttcatcgtg taagaacgga caaagacacg
      421 cccattcaca acgacaaccc agataccgat gacgcgatga tcggaattgc ccccggcgcg
      481 gatgcccata attctgtcct tatgcagggt tgcgataatt tcttcactga atcgtggttt
      541 ggtgttcata aggaaatccc ttttgagttg gtaaattaga ttggcaaaaa ccatccgagc
      601 ccaaaactcg ccagattcat caacaagctc agaaatgtag cttctagtaa taggagcttg
      661 ttcgccggga cgtaatatag cattgcactt tcaataatcc agatcaatat ctcggcaagg
      721 aacagggaaa tcaaataatg ctggaagaag agatttaaca aaacccacaa taaggactgg
      781 gtgatcaggt ttgcaacgat gcttgtatat agaatcggtc gaaatggttt cttgtgccaa
      841 aatgaatatc caaggacaac cgtgccttcg atgatgatcg tgattaggac ggtaaggatc
      901 aagtcgtttt ggaacgtcga attagaaaaa tgccaagccc tgcaatcaaa cccagaccga
      961 tcaaagcgca gatacaggca aggacttcca ggattcccag agagagagaa ggcacaaggc
     1021 taaatttggt ttcaaccatc aggtcttccg ggcgaacgta gactgtgtaa cgagaatcaa
     1081 acccggcctg ttcaaatatg ttgctccgcc gcgtcacacc atccgagaat tcgagttcaa
     1141 gcaaggaata cggagcgaaa ccatagccaa tggcacggca actctgtggt tcacagtaaa
     1201 gtcgttgtgg tcccacctct tccagtggcg tggcatcact acaatcagac tcgttgcatt
     1261 ggaacaggat ccctgaaacg ataccagact cttccacccc tgattcgaat cgaatctgga
     1321 actccatcgt tggtttggga ccagtatcgg caaatgcaac ctggatcggc agaaatgcct
     1381 gcagcaataa cagcactaca aacggtttta tcaatttaga tgccataatt ctcaggggtg
     1441 ttaattaatc gttcgggttt aacgtctcat acgtattagg aggcttatta tggaaataac
     1501 aatggcgacg cccacaagaa atccaaggtc atactcactc ccggcattgt ggacttgata
     1561 catccgcaca tcagagttga agaaggaaac cagcagagtt gccggagaga tgattccatg
     1621 ccatagtcca gctccggcac gcgcgatccg gccagtcgaa tccggctgat tcatcagagg
     1681 gttgggacca ggcgcgctaa gctgcatggt tgaattcggt gtcgtgactt gtacgttcgt
     1741 gccaggtcca caacctgcga ctaaaacaag aaagacaaca gacaaaacga aaaagcgtag
     1801 tttcttcatc tctctgcctc cgtaatattc aattgggatt ttctattctt tgctgcaact
     1861 gcgtagagat ggttgcttcc tgatcgacat aaatcccctg ggcgtctatt caggcacatc
     1921 catagaactt attatacgat aaattgattc cccatcaaag tcgttatcta tacctgcgtt
     1981 ccattaatca tcgccgccgc caccaccggg accgctatta tcttcattgc tattatcatt
     2041 tccgccgcct gagccactgt tgtcttcact tccattgtca ttgctctgat cgcggctgct
     2101 acctgacccg ctattattct gattttcagt accactctct gaaccgctgg agtcgctatt
     2161 tgaattatca ctgccaccgg ggccactatt gttatcgttg ctattgtcat tgccgccacc
     2221 tggcccgctg ttactatcgt tgatgttgtc atttccattg ccaggttcac cattattttc
     2281 attactattg tcgttcccat tgccgggtcc agaggaatca ttttgatttc cactatcgcc
     2341 tgaaccgctg ccgctgttcg atccactacc cgacccgttc cctgaattcc cggcatccgg
     2401 cagatttgcg ccctccggca attggtcaat gcgttctgct aaaaccgatt tatcgctttg
     2461 tgtaatgcca aagacgcgga cggcgtctcc tagcgagaca gccccatcgt gcagttcagt
     2521 ctgagaagta atccggaccg gcactcccgc caccagcaaa tcattcccgt tctgctgcgt
     2581 gacgatccca acgaaatcca cctcgacaga tcggccgatt gcgatcagtt cttgtaattc
     2641 agatagtctt tcagtctcgt actctacttc gagagaatca cgtctttgcg aatcaaacgt
     2701 aagaagtaga aagaggttct cacccgttcg tttgacagaa tacaggttat cacccggcaa
     2761 agcagttgac gaggcgcgta ctaaaccagt accgctgaca aaagcgatgg cgatgatagc
     2821 cagtgcaatc gcggcccggc gcagggatat aaaccaagtg ggcgtctgag ctgactttaa
     2881 ttgagactcg cgcagttcgg ccgcgcgctg cagaagcttt gcccgatttc tctgcagcat
     2941 atcgttggac ggaacagata caaccaggcc cttagcatgg acggaggttt cgagaatcgg
     3001 acgtagttca tccgcaatgt ccggataacg tagcaaagaa gtctcgatat ctgtgccaag
     3061 ttcaatctcc tgcaaacaga cttccaaagc ttcatacagg ttattcatga ctcatctctc
     3121 cgatctggcg ttggagagag gccaacgcgc gaaactgaag ggctttgatc gcattcacat
     3181 tttttttcat atgcactgca gtttcttcaa gtgaatatcc ctgcccaaat cttaaggcca
     3241 gcacatcctg ttgttcgtgg gtgagttgag cataggccct ctgcaccaac tgagtttgtt
     3301 cgcgcaaatc cacctcggcg agaacactca tttgtccatc aggcatcgaa tcggacaatt
     3361 gctccaccgg tctgcgatat ttttggcgca aatagtcatt gacggtgttc gatgccgtcg
     3421 cgatcaacca acccttcagg ctggtctgag gtccctgatt cttttgcgca gcttctaaca
     3481 ggcgcacgaa aacatcgctc gccagatctt cggcgatcct gtcgtctgcg atgcggtacc
     3541 ggatatagcg atagacttca gcgaaatatt gatcgtaaat cgcgccgatg gctgaggaat
     3601 ccagttttcg cagaccgtgc agcgcttgtt gttcattgta cgtagacata ggttgctgtg
     3661 cagcccccca cgctagaatt gctgtcagat aaattcccgc ggcttgcgca tttagtataa
     3721 catcctcaat gatttccact tttgctccga gcatgcaact ctcctacggc aaagcacgca
     3781 cttcgaaaag aacggactga tcatcgcgat aaacttcctt caagccaggg tcttctgcgg
     3841 caacctgcag gaagtctccg tgattaaggt ccgtatggat atattgagaa ccaaaccgac
     3901 tggcaataac ttgtgagggg ttttccacat ctccttgcgt gatctcaacc cagagctcgt
     3961 aaaggtcagg gtcataaagc tgcatatacg tagggtcgag gccaattaga tatgtattgt
     4021 gcgtgttgta aaaaaacaga cgtgggaagt catcccagtc agtttgaaag acgcgtgaat
     4081 tggggggagt attgtcctcc agccaggcag aggcaccagc gtacaagtca tagcttttgg
     4141 atgaatttat cgcctcccgc gtctgtggaa ttgacctggc gatgctcacg gcaacagcag
     4201 ccgatagaag gatcacggga atgcgatcgc gccaactaaa tcttcgttgc aaagcagcag
     4261 aatccgttga tataacggaa ccgagtcgaa cgtcaaatag aggtgcccag gcaaaagcag
     4321 caaagatcag cgcaaagggc ggaaagtatt ccacaaagcg ccgcgcttga aagagcatga
     4381 gtccaaaaag caaagaagtg agaaatgcga aagccgtgcg cacatccatt tttcgccccg
     4441 caagtccgag ggcaagtgtt ccgcttgcaa acgcgatcag
//
LOCUS       DASTJP010000360         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_684745, whole genome shotgun sequence.
ACCESSION   DASTJP010000360 DASTJP010000000
VERSION     DASTJP010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684745"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2494
                     /locus_tag="VFQ23_10715"
     CDS             <1..2494
                     /locus_tag="VFQ23_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HET9907107.1"
                     /translation="FWSGTQMVYGNLFGFARADDVVGHELTHGVTQYESNLFYYYQSG
                     AINESFSDLWGEYYDQTNSQGTDTAAVKWQIGEDVTGYGAFRNMSDPAAFGDPDRMSS
                     PNYHTGDDDNGGVHHNSGLNNKAVYLMVDGGSFNGKTVTALGWDKTAAIYYEAQTNLL
                     LSGSDYSDLYYALQQACSNLTGQHGITSANCLEVKDAIDAVEMNSQPSAGFNTDAPLC
                     SNSSDFATITFSDDIEAGSGKWTFSSIPNALHWQVDSAYFGPYAQSGLHSLYADDIPA
                     EPTDARARFASFVVPSNGYLHFSQAYGFENFEFFSEYYDGGVLEYSTNAGSTWLDAGS
                     LIEVNGYDGTIYSGFGNPLGGRSGFVGSSHGYISTRLNLASLAGQTVTFRWRMGLDEA
                     VAAWGWWVDNIKVYRCGPTNLDVHIGGALMGHYFVPPGSSIRQDFPGIDNGPVKIVGT
                     TTAKILAAMRVIWREPGPRTSYSEMLGLPKEQLSTEYWFPWYNNAVPSSMDQGLRIGN
                     VNATSTAIQVWVGNSLIDSFTLNPGASVRVGYPIDNGPIRVVCSDCSGSEKIIAAMRV
                     IWKEPGRRFSYSEMMGLPKEQLSNEYWFPWYNNATPASVDQGFRIANVSSTENNTVEV
                     RLGNSLLDTITLSMGASVRVAYNVDNGPIKILCTTCTNTGTDKIIAALRVIWKEPGFR
                     ASYSEMMGLPKEQLSSQYWFPWYNNAVPASMDQGFRIANISTTESNTVEVWVGSTQLA
                     TLPLAAGGSTRVGSNVDNGPIKIICTTCTNTGTDKILAALRVIWKEPGYRSSYSEMMG
                     LPHEALSTEYWFPWYNFAAPNSMDQGLRISVP"
BASE COUNT          798 a          748 c          785 g          846 t
ORIGIN      
        1 gttctggagc gggacccaaa tggtttatgg taatctcttc ggctttgccc gagctgacga
       61 tgtggtgggt cacgagctca cgcatggtgt aacgcaatat gagtcgaact tgttttatta
      121 ttaccagtcg ggcgccatca atgaatcttt ttcagacctg tggggcgagt attacgatca
      181 aaccaatagt caaggtacag ataccgctgc ggtaaagtgg cagatcggcg aggatgtcac
      241 cggctacggc gcattccgaa atatgagtga tccggctgcg tttggagacc cggatcggat
      301 gtccagccct aattatcata ccggtgacga tgataatggc ggtgtccacc ataatagcgg
      361 tcttaacaat aaagctgtgt atcttatggt agatggcggg tcgtttaacg gcaagacagt
      421 aacagcgctt ggttgggata agacagccgc gatttattac gaagctcaga cgaacctgct
      481 cctttccggc tctgattact cagacctgta ctatgcactc cagcaagcct gctcaaacct
      541 gaccgggcaa catggcatca cttcggcgaa ttgtcttgaa gtaaaggatg cgattgatgc
      601 cgtggaaatg aattctcagc cttctgcggg tttcaacacg gatgctccgc tttgtagcaa
      661 ttcgagcgat ttcgccacga ttacgttttc cgatgatatc gaagcaggtt cggggaaatg
      721 gacgttttca tcaattccga atgcactgca ctggcaggtg gactcggcct atttcggtcc
      781 ttatgcacaa tctggcctgc attctttgta tgcggatgat ataccagcag aacctaccga
      841 tgctcgggca cgatttgcat ccttcgttgt acctagtaat ggatatctgc atttttccca
      901 ggcttatggc ttcgagaact tcgaattctt ttccgaatat tacgatggcg gtgttttgga
      961 atatagcacg aatgctggtt caacctggct ggatgcaggc tcgctcatcg aagtcaatgg
     1021 atacgatgga acaatctaca gtggctttgg caacccgctg ggcggacgct ccggattcgt
     1081 cggcagcagt catggataca ttagcacccg gctaaatttg gcatcattgg cgggacaaac
     1141 tgtcaccttc cgatggagaa tggggctgga tgaggccgtc gcggcctggg gttggtgggt
     1201 agataacatc aaggtttatc gatgtggtcc aacgaatttg gatgtacaca tcgggggtgc
     1261 gcttatgggc cactactttg ttccacctgg cagcagtatt cgacaggact ttcctgggat
     1321 agataatgga ccggtgaaaa tcgtaggcac taccacagcg aaaattctgg cggcaatgcg
     1381 cgtgatctgg agagagcccg ggcctcgcac cagctattcc gagatgctgg ggcttcccaa
     1441 agaacagctt tccacggaat attggttccc ctggtacaac aacgcagttc catcgtcgat
     1501 ggatcaggga ctccggatcg gaaatgtcaa tgcgacctcc actgcaatac aagtatgggt
     1561 agggaattcg ttgattgaca gtttcaccct caaccccggc gcgagtgtac gggtgggata
     1621 tccgattgat aacggtccaa tccgtgtcgt atgcagtgat tgttccggca gtgaaaagat
     1681 cattgcagcg atgcgcgtga tctggaagga acccggacgg cgttttagtt attcagaaat
     1741 gatgggctta cctaaagaac aactttccaa tgaatactgg ttcccctggt ataacaatgc
     1801 tacccccgcc tcggtggatc aagggttccg tattgctaac gtcagctcca ctgagaataa
     1861 taccgtcgaa gtgcggctgg gcaattcact tttagatacg attaccctta gtatgggggc
     1921 gagcgtccga gtagcttaca acgtggataa cggtccgatc aagatccttt gcacaacctg
     1981 tacgaatacc gggaccgaca agatcatcgc tgccctgcgc gtcatttgga aggaacccgg
     2041 ttttcgcgca agttattcag agatgatggg gttgccaaaa gaacaacttt ccagtcaata
     2101 ctggttcccg tggtacaaca atgcagttcc tgcatcgatg gatcaaggct tccggatcgc
     2161 taacatctcc accactgaat ccaacacagt tgaggtctgg gtcggcagca ctcagttggc
     2221 aacccttcca ctcgccgcag gaggcagcac acgcgttggg tctaacgtgg ataatggtcc
     2281 aattaagatt atctgcacca cctgtaccaa cacaggtacc gataagatcc tggctgcgct
     2341 gcgtgtaatt tggaaggagc cgggttatcg atccagttac tcagagatga tgggtctgcc
     2401 ccacgaggcg ctttccacag agtattggtt cccctggtac aactttgctg ctcccaactc
     2461 catggatcag ggtctcagga tcagtgttcc atagagtctt cggacctgaa aattcatctg
     2521 taatctccct ttgaatttca caagcatcca tcactggcaa tgattctgag tcattgccag
     2581 tgattctatt ttgactgcta tcttcatcct cgatgctcta ttttagataa atagttgact
     2641 tttaagttgg attcattata atgaatccag cacactgcac tgtgatgact gaaacgcatt
     2701 aaataaatag agactaggag gagaatatta acgataagtc taattacttt ccaagctgtt
     2761 gtcattggac accaacctga caaatacagg tttacataat ctttaattct ccagtacatg
     2821 agaatgacgg tcaattcctg tttcggtggg tgttgcaaac tctgcgataa cctatctctg
     2881 agtcacaaac tcaggactat atttttggtt tcccggatac atctcaaact tctttggaga
     2941 aaggaatatt aatttatcgt attgtagaag cccatttctt ataaaccagc aaagcgttgt
     3001 ttttcaagct gatggggcaa cggcgctgga ttcaaggatc aataggagct actatgaaca
     3061 ttcaaccttc tcaaattttc ctacgcaagt ttctattctt cgcgttgttg gtaacaatca
     3121 gtctttcact gattatcgta aacacatctt cttccacagt gggagcacaa gcagcct
//
LOCUS       DASTJP010000361         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1497683, whole genome shotgun sequence.
ACCESSION   DASTJP010000361 DASTJP010000000
VERSION     DASTJP010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497683"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1254
                     /locus_tag="VFQ23_10720"
     CDS             160..1254
                     /locus_tag="VFQ23_10720"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HET9907108.1"
                     /translation="MNNDTQHGYLLLADISGYTSYLVGTELDHARDVLTELLELIVQR
                     FKPVLNIAKLEGDAVFAYIPKEKIIRDETLLEMLESTYLDFRDRVEGIHRRTTCQCNA
                     CKLIPSLDLKFIMHFGAYLLQNVSGIRELVGSDVNLVHRLLKNHVSESTGWKAYALFT
                     DAAITQMNMKLENLHKQVESYEHLGEVKTFNMNLHERYKELKEVQRVFISPEEADWAS
                     TRTIAAPPHVVWEWMNDIQRRLLWETLHDVQPLLRPGGRMGTGAQNHCAHGKNVFVET
                     IVDWRPFKTFTIDTGSAFMSRYLEDHGGKTRMNIYMKMKLQMPRWVTRPLVGLMLRIG
                     KVDQQFDTLVRLIETEANKSGKVNDEQNRP"
     gene            1232..1537
                     /locus_tag="VFQ23_10725"
     CDS             1232..1537
                     /locus_tag="VFQ23_10725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907109.1"
                     /translation="MMSRIDPENNEPRALLEFADFSDKHVLEIGCGDGRLTWRYADHA
                     AHVTAIEPSAEQIKLAKDNLPSHLRGRVEFHSATLEDFAAKSKPSIVGLVILSYSLC"
     gene            1553..1996
                     /locus_tag="VFQ23_10730"
     CDS             1553..1996
                     /locus_tag="VFQ23_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907110.1"
                     /translation="MVHALEEIHRLLKPGGFLIDIHPVAEHSSVEIHQNGKIDLVGQL
                     EVSQWCVDFQEADKALDEIIQRGIFTIEQKGMFETLTYYDSATEMGTAFKQSIQKYVR
                     EAEPVKDEVSHVEMLAAQTEELMRAAGGAQLLLRERDHISRLRPT"
     gene            2087..2455
                     /locus_tag="VFQ23_10735"
     CDS             2087..2455
                     /locus_tag="VFQ23_10735"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9907111.1"
                     /translation="MAIQAIIINQDQQVLLLSSPTRKQGWQVVSGALKAGETLLAGTL
                     REVYEELGKNIQVRPLGTVHIETFHYDEQVRFMVSSYYLFAYQGGRIIPGDDMKGSKH
                     RWWSMKKTGPIFTVRPNPGY"
     gene            complement(2537..3349)
                     /locus_tag="VFQ23_10740"
     CDS             complement(2537..3349)
                     /locus_tag="VFQ23_10740"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9907112.1"
                     /translation="MDKRFFSDNLWYHFLTGEFKVPPPMFIRVLSGTVGKVYKILPSN
                     PHCSECGIPMAGAGGNSLRFMGSAPSSFSSRLCSGCERAIRKQEGGAEVELTMVFADV
                     RDSTPLAEEKGVTGFKDIINRFFKESSKVLIEHNAMVNRLVGDQVIGLFVPRFAGKNH
                     AQTALEATMDLLRVTGHGQGKDPWISVGAGIHTGPAYVGAVGSANGVNEVAVLGSSAN
                     LCARLSSKAAAGEILISTDSVKFGDLDVDGLETRSLELKGVSQPVSVSVVKV"
     gene            complement(3647..4444)
                     /locus_tag="VFQ23_10745"
     CDS             complement(3647..4444)
                     /locus_tag="VFQ23_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9907113.1"
                     /translation="MSFEFFTTDDHVKLGYTDKGKGVPVILMAGYSAPATSWYREEKS
                     LVKHGYRVIAFDRRSHGSSENPPFGQDMVTHGKDLAALIRHLQIKKAFLVGQSQGASC
                     MWSYISQFGTEHLYGVISVDQTPKMINDESWPYGMTGLTRETHPTFFDLPLPKPNKKS
                     LGIFLIGVLVRGKKYLNFDHAGTKPLLLDHADADWRETLKKTDVPALFVAGEESPFWP
                     KDHAATSAALCPQGESAVVAGAGHAVNWECPKEFSAIMLEFLSRVSK"
BASE COUNT         1241 a         1080 c         1084 g         1112 t
ORIGIN      
        1 ggaggttggt cgcgcgggcg acatatgagg cagaagggga tgacaaaact ctcttgacca
       61 tgaacgttga attccctgat ttagatcaac tggatacaga aatgctggtc aacgcgatgc
      121 aaaatagcat ccgcaatatc aatcaactcc tcatggacaa tgaataacga cacgcaacac
      181 ggctatttac tattggcaga tatctctggc tacacatcct atttggttgg aacggaattg
      241 gatcacgcac gggatgtatt aaccgaactt ttggagttga tcgtccaacg ctttaagcca
      301 gtgctcaaca tcgcaaagct cgagggtgat gctgtctttg cctatattcc caaagagaag
      361 atcatccgcg atgagacact gctcgagatg ttggagtcaa cttatcttga ttttagggat
      421 cgtgtggaag gcatccaccg ccggacaacg tgtcaatgca atgcttgtaa gttgattccc
      481 agcctggact tgaaatttat catgcatttt ggtgcgtatc tattgcaaaa tgtatcgggg
      541 atcagggagc ttgtcggctc ggacgtaaat cttgtccatc gcttgctgaa aaaccatgtc
      601 agtgagtcaa cgggctggaa ggcatacgct cttttcaccg atgcggcaat cacgcaaatg
      661 aatatgaaac tggaaaatct acataaacag gttgagagct acgaacacct gggcgaagtc
      721 aagacgttca acatgaacct gcacgaacgc tacaaggaac ttaaagaggt ccagcgtgtt
      781 tttatttcac ctgaagaggc tgactgggca tctacgcgca caatcgctgc gcctccgcat
      841 gttgtgtggg aatggatgaa tgacattcaa agacgcttgc tgtgggaaac cctccatgat
      901 gtccaacccc tgcttcgacc cggcgggcga atgggcacag gtgctcagaa tcattgtgca
      961 catggaaaga atgtctttgt cgaaacaatt gtggattggc gtccctttaa aacgttcaca
     1021 attgataccg gttcggcttt tatgtcgcgg tatctagagg atcatggtgg aaagactcgg
     1081 atgaatatct acatgaaaat gaaattgcag atgcctcgct gggtgacccg tccgttggta
     1141 ggattaatgt tgaggatagg gaaggtcgat caacaattcg atacattggt gaggttgatt
     1201 gagacagagg cgaataagtc tggaaaggtt aatgatgagc agaatagacc ctgaaaacaa
     1261 cgaacctcgt gctttactcg aattcgcaga tttcagcgat aagcatgtgc tggagatcgg
     1321 ctgtggagat ggtcgcctga cctggcgtta tgcggatcat gccgcgcacg tcaccgcgat
     1381 tgaacccagc gccgagcaga ttaaactggc aaaggacaat ctgccgagtc acctgcgggg
     1441 cagggttgag tttcattctg ccacgctcga agactttgcc gctaaaagca aaccctcgat
     1501 agtcggcctc gtgatcttgt cctactcatt atgttgaatg gaatatggga gcatggttca
     1561 tgctcttgag gaaattcatc gcctgctgaa acctggtggt tttctaattg atatccatcc
     1621 tgtagctgag cattcgtctg ttgagataca ccaaaatgga aaaatcgacc tggtggggca
     1681 attggaagtt agtcaatggt gtgttgactt tcaagaggca gataaagcat tggacgagat
     1741 cattcagcgc ggaatattta caatcgagca gaaagggatg tttgaaacgt taacctatta
     1801 cgactctgca acagagatgg gtacagcgtt caaacaatcc atccagaaat atgtcagaga
     1861 ggcggagcct gtaaaagatg aagtatcaca cgtagagatg ttggctgcgc agacggagga
     1921 actgatgcga gccgcaggcg gagcacagtt gctgttacgt gagagggatc acataagtcg
     1981 cttaagaccg acctgagaaa tgattggatg aaaaggtgta atctcaagta aacaacccat
     2041 catagtcacg cttggttcaa aacctcgcca attcgccact tcggctgtgg caattcaagc
     2101 aatcataata aaccaggatc aacaagtgct tcttctttcc agtccaaccc gcaaacaagg
     2161 ctggcaggtt gtcagtggcg cactcaaagc tggcgaaacg ctcctggcag gcacgcttcg
     2221 tgaagtttat gaggaattag gaaagaatat ccaggtgcgt ccgctaggca cagtgcatat
     2281 tgaaaccttt cactatgatg aacaggtccg atttatggtg agcagctatt atttatttgc
     2341 ttatcaggga ggtcgcataa tccctggcga cgatatgaag ggtagtaaac atcgctggtg
     2401 gtcgatgaag aaaacaggac cgattttcac cgttcgacca aaccctggtt attaaaacgg
     2461 gctatcgagc tttataacct gtgggaaaga gaaaatgacc tgccattaca ggaagcttta
     2521 tagctgacac ccttttctaa accttcacaa cgcttactga aacaggttga cttacaccct
     2581 tcaactcaag cgaacgggtt tccaatccgt ccacatccaa atccccaaat ttcacactat
     2641 ctgtactgat caaaatttcc cccgccgcgg ctttggatga caaacgagcg cacaagtttg
     2701 ccgaacttcc caagaccgca acttcattta caccgttggc tgatcctact gcaccgacat
     2761 atgccggccc tgtgtgaatc cctgcaccga ctgaaatcca tggatctttc ccttgaccat
     2821 gtcccgtaac tcgtaaaaga tccattgttg cttcaagggc agtctgcgca tggttcttcc
     2881 ccgcaaagcg cgggacgaac aaaccaatta cctgatcccc gaccaagcga ttgaccatcg
     2941 cattatgttc gatcaaaacc ttgctgcttt ccttgaaaaa tcgattgata atatccttga
     3001 accctgtcac ccctttttcc tctgccaaag gtgtggaatc gcgcacatct gcaaacacca
     3061 tggtcaactc aacctccgcg ccgccttcct gcttacggat agccctttcg caaccactgc
     3121 aaaggcgcga actgaaactt gagggagcag agcccatgaa acgcagggaa tttcctcccg
     3181 ccccagccat gggaatgccg cattcagaac aatgcgggtt acttgggaga atcttgtata
     3241 ctttccctac agtacctgaa agcacccgta tgaacatggg aggaggaacc ttgaactcac
     3301 ccgttaagaa gtgataccac aggttatctg aaaagaacct cttatccatt aaaattctcc
     3361 acttcaatat taccatgcaa aacaagagga ttttacttat taagattacc tgattggcga
     3421 gtaatccgtg ggaacagaaa aattaaacaa gcgatggatg tgggaaggac atcatcctgg
     3481 atggggtgat taatctgcgt ggtgatttgt cccttcccaa tccccttcgg ggatgtggcg
     3541 agggaagagg aggagatgga agtgtaagat atgaagattt ttatcgaact taggcatgta
     3601 attgcaatgt acgctgtctg atcgaaaacg agaacatgct tcccagctac ttacttaccc
     3661 gggataggaa ctccagcatg attgcactaa attcctttgg acattcccag ttgaccgcat
     3721 gacccgcccc tgctaccacg gccgattcac cctgcggaca tagcgcggcg ctcgtggcag
     3781 catggtcttt gggccagaag gggctttcct ctcccgcgac gaacagagcc ggcacatccg
     3841 ttttcttgag cgtttcccgc caatcggcgt ccgcatgatc taacaacaac ggttttgttc
     3901 cggcatggtc aaagttcaaa tacttcttcc cgcgcaccaa caccccaatc agaaatatgc
     3961 ctaaagattt cttgttaggc tttggaaggg gtaaatcaaa gaaggtggga tgtgtttccc
     4021 tggttagacc cgtcatgccg tacggccaac tttcgtcatt gatcattttg ggtgtctggt
     4081 caacactgat cacgccatac agatgttccg taccgaattg tgaaatgtag gaccacatac
     4141 aggaagctcc ctgagattga ccgaccaaaa acgctttctt gatttgcagg tgtcgaatca
     4201 acgcagcaag gtctttgcca tgcgtcacca tatcctgccc aaacggtgga ttctcagaac
     4261 tgccatggga gcgtcgatca aaagcgatga cacgatagcc atgctttact aaagatttct
     4321 cctccctata ccatgaagtg gcaggagcac tgtacccagc catgaggatc accggtacgc
     4381 ccttgccctt atcggtatac cctagtttca catgatcatc cgtcgtgaaa aattcaaagc
     4441 tcatgatgac ttccttttat gaagacctaa aagtatgttc taagcagcaa ccgttggtcg
     4501 agtaggcggt gcatttc
//
LOCUS       DASTJP010000362         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_217609, whole genome shotgun sequence.
ACCESSION   DASTJP010000362 DASTJP010000000
VERSION     DASTJP010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217609"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFQ23_10750"
     CDS             <1..626
                     /locus_tag="VFQ23_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907114.1"
                     /translation="DLVESVLVRFRSMNIRKLSWLTHENVPSAEIHKVLLAHGLTFSD
                     TFATEMAVDLSLLPDGLPTHPGLRIVPVDGEHVLKQWIHVAGIGFRIEESFEKVWFDF
                     FADAIFDRRFQTYLALLDGKPVATSQLFLSEGVAGIYNVSCVPDVRGQGIGSAVTLAP
                     LLEARQMGYRIGILQASKGGYNAYRRLGFQDYGKLSVYLWENDRGVD"
     gene            662..1105
                     /locus_tag="VFQ23_10755"
     CDS             662..1105
                     /locus_tag="VFQ23_10755"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9907115.1"
                     /translation="MGKFTVSVFINRSQQDVFDFLSNPAIKPKWMPMLESASWTSSGE
                     PGVGSTVRAMMKMAGKETEMRLEITQWDVPNRYGYKVLTVSFPMKAMAHSFRLEPEDR
                     GTRVSQVGEFEMVSIMRFAAGWMGKIAAKLNGSELKTLKQLLEAG"
     gene            1152..2318
                     /locus_tag="VFQ23_10760"
     CDS             1152..2318
                     /locus_tag="VFQ23_10760"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HET9907116.1"
                     /translation="MSTNTQHGYLVIADISGYTSFVAKTELEHSQEILTELLELLITR
                     LQPLMTISKLEGDAVFVYAPEEIFVRSETLIDFIESIYVAFRDKQLSIKRSTTCTCNA
                     CRNIPSLDLKFFIHCGDYIQQTIANIKELIGSDVNLVHRLTKNHVTEATGWRAYMMFT
                     EQCMKHNGLSLTDAHTQMEVYESLGEVRTFNIDLHTRYKEICEARCIVLEENDADFIF
                     QIDFLTPPSVTWEWLHNPEKRNIWGGVPNAWSVGDRPLGRPGVGSSNHCAHGKSFSTQ
                     VVVDWHPFEYYTIHSLEKGKHTFTETLRFEALPNGGTRVRHVGKMHMPLPRFIRRAAL
                     KFVLITQHHYDKLIARASQLASEEFTKTAGDHDAISSTSVSILLQEAKGKHFST"
     gene            2402..>2702
                     /locus_tag="VFQ23_10765"
     CDS             2402..>2702
                     /locus_tag="VFQ23_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9907117.1"
                     /translation="MSTENEMRIKVIVELEARPGKRAELKSLLESVVAKYGPSQPGFL
                     DSTRYEVLDNPNILVEIADWASADVRASAMQRAMADGAYAPLEDLLAAPFRATVIR"
BASE COUNT          706 a          647 c          698 g          651 t
ORIGIN      
        1 acgacttggt tgaaagcgta ctggttcgct ttaggtctat gaatatcagg aaactctcct
       61 ggctgactca tgagaatgta ccatccgctg aaatacataa agttctgctt gctcatggtt
      121 tgacattcag cgatacgttt gccactgaga tggcggtcga tctctcgctg ttaccggatg
      181 gtttgccgac tcatcctggc ttgagaatcg tacctgtgga cggagaacat gtattgaaac
      241 agtggatcca tgttgcaggt attggtttca gaatcgagga gagttttgag aaggtttggt
      301 ttgatttttt cgcagatgcg atttttgaca ggcggtttca aacctatctg gccctgctgg
      361 atggaaagcc agttgccaca tcgcaattat tcctctctga aggcgtcgcg ggaatctata
      421 atgtttcctg cgttccagat gttcgtggtc aggggattgg ttcggccgtc acattggctc
      481 cgctgctcga ggcgcgccag atgggatatc gaattggcat tctacaggca tccaaagggg
      541 gctacaacgc ataccgccgc ctgggatttc aagattatgg caaattgagc gtttatcttt
      601 gggaaaatga tcgcggtgtt gactaataag atgagagtag ggattcaaag gagaacagac
      661 aatgggcaag ttcacagtaa gcgtttttat taaccgttca cagcaggatg tattcgactt
      721 cttgagcaat cctgcaatca agcctaaatg gatgccgatg ttggaatcag catcgtggac
      781 gtccagcggg gagcctggtg taggttcaac tgtcagagca atgatgaaga tggcgggcaa
      841 agagacggaa atgcggcttg agattactca atgggatgtg cccaaccgtt acggctataa
      901 ggtgctcacc gtgtcgtttc ctatgaaagc catggctcat tcatttaggc tggaaccaga
      961 ggaccgcggt actcgagtat ctcaggttgg cgagtttgaa atggttagca taatgaggtt
     1021 tgccgcagga tggatgggta agatagctgc gaaattgaac ggaagcgaac tcaaaacctt
     1081 aaagcagttg ctggaagcgg gctgatgcgg cacacagaac accttctagg cttcgtgggc
     1141 atgttttgaa tatgtcaaca aacactcaac acggctatct tgtcattgca gatatctctg
     1201 gctacacatc gtttgtggca aagacagagt tggaacactc tcaggagatt ctcacagaac
     1261 tgctcgaatt gctaatcaca aggctccagc cgttgatgac gatctccaaa ctcgagggcg
     1321 atgctgtatt tgtatacgcg cccgaagaaa tattcgtgcg cagcgaaaca ttgatcgatt
     1381 ttatcgaatc catctatgta gcttttcgcg acaaacagct ttccataaaa cgctcgacaa
     1441 cctgcacctg caatgcctgc cgtaacattc catcccttga tctgaaattc ttcatccatt
     1501 gcggcgacta cattcaacaa acgatcgcca acatcaagga actcattggc tcggatgtga
     1561 acctggttca tcgcctcacc aaaaaccacg ttacagaagc gaccggctgg cgggcatata
     1621 tgatgttcac cgaacaatgc atgaaacaca acggtttgag tctcaccgat gcccacaccc
     1681 agatggaggt gtatgaaagc ctcggcgagg tcaggacctt caatattgat ttacacacgc
     1741 gttacaagga aatttgcgaa gcccgctgta ttgtgcttga agagaatgat gcggacttca
     1801 tctttcaaat agatttcctc acgcctcctt ctgtgacctg ggaatggctc cacaacccgg
     1861 agaagcgcaa catctggggc ggtgttccca acgcctggtc ggtaggtgac cgaccgctag
     1921 gacgaccggg tgtcggttca agcaatcact gcgcacacgg caaatctttt agcacacaag
     1981 tggtggtgga ctggcatcca tttgaatatt acaccatcca ttcattggag aaaggcaaac
     2041 ataccttcac agaaacgctg cgttttgaag ccttgcccaa tggtggcaca cgcgtccgtc
     2101 atgtgggcaa gatgcacatg ccactgccgc gtttcatccg cagagctgcc ctgaaattcg
     2161 tgctgatcac ccagcatcac tacgacaaac tgatcgcccg cgcctcgcaa ttagccagcg
     2221 aagaattcac gaaaaccgca ggcgatcatg acgcgataag ttcaacgagc gtgtcgatcc
     2281 tgcttcaaga agcaaaagga aagcacttct cgacatgaca ggtctgatcc atcctgactg
     2341 attgtctgca acacgaccct atgcggtatg aaagagatcc aatttctcat aggaggaacg
     2401 tatgtcaaca gaaaatgaaa tgagaatcaa agtgatcgtc gaacttgagg ccagaccagg
     2461 caagcgggct gaactcaaga gcctgctcga aagcgttgtg gcgaaatatg ggccaagcca
     2521 gcctggtttt ctggacagca cacgttacga ggtgcttgat aaccccaaca tactggttga
     2581 aattgccgat tgggcgtcgg cggatgtgcg ggcatcagcc atgcagcgag ccatggccga
     2641 cggtgcctat gcacctcttg aagacctgtt ggctgcgcct ttcagggcaa cggtcatccg
     2701 gc
//
LOCUS       DASTJP010000363         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1374423, whole genome shotgun sequence.
ACCESSION   DASTJP010000363 DASTJP010000000
VERSION     DASTJP010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374423"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="VFQ23_10770"
     CDS             complement(<1..1625)
                     /locus_tag="VFQ23_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907118.1"
                     /translation="MKAYWEKFKVNLSSPRSVPIVLFIIAFLTYGLFFWERGFYWDEA
                     PWTWIYYRLGPDALTKTFSTSRPFWGMIYQITMPLLGPHPWRWQLLMVIMRWLTAVLA
                     WLLLRQVWQKDERPALWTSLLFLVYPGLGQNFISLMYTHFYIVLNCFLLSLYLSVLAL
                     GTERSDSEDEARMWRKPLWLTLAALAFSIINLLTMEYFYFMEFLRFILFWIVLDGEWQ
                     QRLRRAVMLFLPYFGVMIGVTIWRLFFFENQNASYSYVTLDLIRQNLLLGISTFFRYI
                     LRDLWVTVPQAWAFPFVPTSASELGTRVTLVSAALALILTPLIGIYLYYYSNRKQTER
                     AQTAELFLLGFFTWLFAGVSFWLVGIEPQLHFSADRFTMPFMLGSSLILVAFIRLLGS
                     KPLLPYGMLAVLIAFSVGKQFETNVAYVRDWDLHHNFFWQMNWRIPALERNTTILTND
                     LPVTYFSDNSLSGPLNWIYGREDEMDHILYFASVRINRGLPDLAPGLAIEQNYLARTF
                     YGNTSQMVVLNYSPPGCLRVLDSDIDFDNRLLDP"
     gene            complement(1622..2227)
                     /locus_tag="VFQ23_10775"
     CDS             complement(1622..2227)
                     /locus_tag="VFQ23_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907119.1"
                     /translation="MDYELVPFSQVNEGDLKQLALLHHSVMHTLLSDLGPPIVLRYYQ
                     IARSDKSVVGMYAINSSGEITGWAMGSPHPDRINSRLRTPLLWFAVQMLRVMFTRPSV
                     LLQLISSLLSSSAETEMKNDGVELTYIGVAPDQRGKGLGKTLLRAFVEASRSGGYRSV
                     VLSVETDNTAAIRLYEKSGFRLTNTFSEGHYQRHRMELILE"
     gene            complement(2233..>2427)
                     /locus_tag="VFQ23_10780"
     CDS             complement(2233..>2427)
                     /locus_tag="VFQ23_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907120.1"
                     /translation="NEVFKMKWKPRKQLLAYWYALSPGHALPELNQKNPKFEMAIWLW
                     KRLPSFVVRTLGPFLIKGLA"
BASE COUNT          751 a          585 c          555 g          536 t
ORIGIN      
        1 ggatcgagta acctgttatc aaaatcaata tcagaatcaa gtactcgcaa acaccctggc
       61 ggtgaataat tgagaacaac catttgtgat gtgtttccgt aaaacgtccg cgccaggtag
      121 ttctgttcga tagccaagcc gggcgcaaga tctggcaagc cgcgattgat tcgtacagag
      181 gcaaagtata ggatgtgatc catctcgtct tcgcgcccat agatccaatt cagaggtcca
      241 gataatgaat tatcactgaa ataggtcacc ggtaaatcat tggtgagaat cgttgtattc
      301 cgctccaggg cagggatgcg ccagttcatc tgccagaaaa agttgtgatg gagatcccaa
      361 tcacggacat acgccacatt cgtctcaaat tgctttccca cagaaaacgc gatgagcacg
      421 gctaacatgc cataaggcag aaggggtttg gaacccaaca gacggatgaa cgcgaccagg
      481 atgagactcg accccagcat aaaaggcatc gtgaaacggt ctgctgaaaa atgtaattgc
      541 ggctcgatgc ccaccagcca aaacgatacg ccggcaaata gccatgtaaa gaaacccaat
      601 aaaaacaact ctgctgtttg agctcgttct gtttgtttgc gattgctgta ataatataaa
      661 taaatgccaa ttagaggagt aagaattaac gccaaagcag cagagactag agtgacacgc
      721 gtacctaatt cactcgcact ggttggtaca aaaggaaacg cccacgcctg tgggacagtt
      781 acccacaaat cgcgtaggat gtagcggaaa aaagttgaga tcccaagcag cagattttga
      841 cgaattaagt ccaacgtaac ataactgtaa ctggcgtttt gattttcaaa aaagaataac
      901 cgccagatcg ttaccccgat cattactcca aaataaggaa gaaaaagcat cacagcacgg
      961 cgaagtcttt gctgccattc tccatccaac acaatccaga ataagatgaa acgcaggaat
     1021 tccatgaaat aaaaatattc cattgtcaac aggttgatga tggaaaaggc aagtgcggcc
     1081 agggtcagcc aaagaggttt tctccacatc cgcgcttcgt cttcgctatc gctccgctca
     1141 gtgccaaggg caagcacaga caaatatagc gacaacaaaa aacaattgag aacgatataa
     1201 aagtgtgtat acatcagaga gatgaaattc tgccccaacc ccggatacac aagaaataaa
     1261 aggcttgtcc acaatgcggg gcgctcatct ttttgccaga cctgtcgcaa taacagccag
     1321 gccaaaaccg cggtcagcca gcgcatgatg accatcagca actgccaccg ccagggatga
     1381 ggtccgagca gaggcatggt gatttgatag atcatccccc agaacggacg gctcgtcgaa
     1441 aacgtctttg tcagtgcatc ggggccgagt cgatagtaaa tccacgtcca gggcgcttcg
     1501 tcccagtaga agccgcgttc ccagaagaat agtccgtagg tgagaaaagc aataatgaaa
     1561 aggacaatcg gtacgctacg cggcgacgat aaattgacct tgaacttttc ccaatacgct
     1621 ttcattccaa tatcaattcc atgcgatgac gctgataatg accttctgaa aaagtgttcg
     1681 tgagcctgaa ccctgatttc tcatacaagc gaatcgctgc tgtattatct gtttcaacac
     1741 tgagtacaac ggaacgatag ccaccggaac gactcgcttc cacaaatgcg cgcagtagag
     1801 ttttccctaa tcctttgcct ctttgatctg gcgcaactcc gatgtacgtc agctcaaccc
     1861 catcattttt catttctgtc tcagccgagg aggaaagcaa cgaagagatc aattgcaata
     1921 acaccgaggg gcgagtgaac ataactcgaa gcatttgaac agcaaaccag agcaaaggtg
     1981 tacggagacg tgagttgatt ctatcaggat ggggtgagcc cattgcccat ccggtaattt
     2041 cgcctgaaga attgatcgcg tacataccaa ctacagattt gtcactacgc gcaatctgat
     2101 aatatcgcaa tacgattggc ggccccaggt cggaaagaag ggtgtgcatc accgaatgat
     2161 gaagcaatgc caattgtttc agatccccct cgttgacctg agagaatggg acaagctcat
     2221 aatccataaa ttttaagcga gacccttaat aagaaacgga cctagcgtgc gcacaacaaa
     2281 ggaaggcaag cgtttccata accagattgc catttcgaat ttggggttct tttgatttag
     2341 ctccggaaga gcatgacccg gcgaaagggc ataccaatac gccagcaatt gcttgcgggg
     2401 cttccatttc attttaaata cctcatt
//
LOCUS       DASTJP010000364         7208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_119548, whole genome shotgun sequence.
ACCESSION   DASTJP010000364 DASTJP010000000
VERSION     DASTJP010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119548"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFQ23_10785"
     CDS             complement(<1..184)
                     /locus_tag="VFQ23_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907121.1"
                     /translation="MPETDQFNCQAQDAVIWIGLDAFRELSDWIMAANDHRLLRNRPE
                     VLSQEVSVIKRAMGDGV"
     gene            complement(139..1680)
                     /locus_tag="VFQ23_10790"
     CDS             complement(139..1680)
                     /locus_tag="VFQ23_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9907122.1"
                     /translation="MANPAIQELIENINDDLPRSTLYGEKAPALMEAFDVERMTPVLQ
                     EALLNSNPSQYSILKCVPGKALYLPDHIINMQYKLTIQHHINKQTAETLINAQLFPDL
                     VECTTYLEKILMPSAARMTERPEIKPFARPVAIIEHLHMALSVFPIDGLIPTLLDATD
                     PAKMASILEEALPEALSGEFVIKDVRLLPAHYGRYKRCVLRYSVDGIQTETQTPQNVT
                     VYGKIDANGLGEQIGPILSLLREYLQKPDLPYRFRVPHSLGYLPDLKLLLMEALPGKP
                     FFKELLKTWTGTRDGKPSTERDVALEQAIRACAWIAAILHGTNIKLGLQNTLETQVAN
                     LLEEVDVLCQALPEVGAEVRSWIDQTVEFAQAYPAMPLCFSHGDFRYTQLIFDGTNGG
                     LVDFDSICQAEPAQDLGQYLAYQRLSIVRVRDPNSPLSPEASEHLCAIFLDAYINASE
                     GWITDEEVFRGRVAIYELISLIRLALHSWEKLKGSRLKQTLSLLEERIECQKQISLIA
                     KHKMP"
     gene            complement(1689..3719)
                     /locus_tag="VFQ23_10795"
     CDS             complement(1689..3719)
                     /locus_tag="VFQ23_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907123.1"
                     /translation="MSKKSSDDQKGSSSGLNIRPLVGMITRFSGNSRRIFILSLVMLI
                     LESATAVLIPLLVPGAVVNLAAESLAALDVNSVVTTLIFITLGLIILTMLNSLCDSLS
                     AIYLAQGGRKVGYNMRVFLYNHLQKLSLSFHGQSRTGDILSRVTSDVAALEDFIISNL
                     SDFLGSILNIVILMFAMCLGAVTVWRPSLLQDSCNRWIPSTFANVCLNLKPPEEVVGT
                     VGTPTVFIIPLVALAIIPVMALITNYFTGRIKSASKRLRSTEGELASAAQEMLASIRV
                     VQVYGQGDYEQSLFSGQSQKAMNAALEAAAYQARFKWAMCVLAAVVTAIIIWIGVGII
                     RANPGSIQPGILLTFIVWVDNMFKPAKKLISQWNAFGKLYASLERIGDLLDLTPEVHD
                     KPDALTAPSFHGRIEFRNVVFSYPSVETSKKKGEGKARPTLNGLSFDVRPGQVVALVG
                     HTGAGKSTVAQLLPRLYDPDEGQILIDGHDIRDYSLESLRAQMSMVLQESILFTGSIL
                     ENIGYGRPDATGIEIIEAAKDANAHEFISKFSDGYYTVLAERGSNLSGGQRQRIAIAR
                     AFIRDTPILILDEPSTGLDAESTDLVLQALRKLMKDKTTIIISHELNLIRNADQIIVI
                     KEGQIEQIGTHDELIRAGGLYANLYIMQSSQRAMENNPLPISETADPKLGKG"
     gene            complement(3758..5026)
                     /locus_tag="VFQ23_10800"
     CDS             complement(3758..5026)
                     /locus_tag="VFQ23_10800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9907124.1"
                     /translation="MVESLSNKQIGYLLRSYPRLSQTFILNEILALEKIGVSIQIFAL
                     TNPHEKVVQMQVDQVQAPVHYLDESIQSRRFGNMLKEHMEVSMRHFTGYFRSLLYVVM
                     HPVIDQGYTASSRWECFHQAVHLIHVLRVQERETGKKIDHLHAHFAHDPTLIAYLANL
                     MTGLPYSFTAHARDLYQIPEQILTDRIRTSQGVITCCRVNMDYLNQVAPSEQSKFSLI
                     YHGVNLDDFRASSSNGANSTSEVPIILSVGRLVEKKGFLDLLQALLMVKEGGLQFQCT
                     IYGGGPLSEKLEQWIEEHDMADDVRLMGDITQQELISIYQNATIFALTPMQTEDGDRD
                     GIPNVLVEAMAVGLPVVTTAVAGIPELVDDNLNGLLYQPHDVNGISSGILELLRDAEK
                     RRQLGNAGAKKVNEQFDIALAANRLQNLFA"
     gene            complement(4948..7146)
                     /locus_tag="VFQ23_10805"
     CDS             complement(4948..7146)
                     /locus_tag="VFQ23_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9907125.1"
                     /translation="MQRRFSELPAFAGFGDQVRLWEIFPVRYKPGLHCVIRYTARTPS
                     GNKMFYGKSFTGDAEQLYKTIIDLHTCSEENPEMPLISAPVAVWPDLEMIVQAAVPNG
                     IEFSHFAYDQAYDASLREDWFVKAGRALGVFQDNSTVPGETKTVYDDLQDLHEYTVII
                     GKVRADLAAKYEEVIQHINTKVSQFQEPKPVASHGAMRTDQYIVQGDRLAMIDLDSYC
                     WANPARDLGNFLAYLCWKAIRQPEHAQFVERAGRAFLEGYLSVHADIDERWLSVYQAA
                     SLLKIAGRRFRSLTYLEWPLIIHLIQAAHSAITEDLANLEAHTITDLKGTLVAHLRTA
                     TGKTKFPHTFQDVPFPALWSALNAEIMNSNLTPMLSDICSATSSRNVHRVKLLAYKPG
                     KRGVIRYDLDQIECQKHDEVFGKLYPEPHLSERAYKVLKTLHDEVFRDATDLGVPEPL
                     GVIPDLSMLVFIPAGGKLLGDYIAKRSLDGDDVIRAMQLSGKWLAQLHTHRFPLEKEF
                     KVENEIDNIRDWAELISQKYPKERQAATHIAEYLVQRTTELDFSAHAPIHKDFHYEHI
                     LIDEGLNLKVFDFDEMRLGDPNFDLAHFCANFYLLAYRNNEHTAKFTDLQNLFLDAYS
                     SGTGWQWDEKFLFFYIYSCLKIAKQLCKFRGPRPWPEGDEQQAQVWLMIEQGLIMGES
                     AMTRASQKNIEVPVYEFAEIKKATRWSKAYQISKSGTSSGAILVSHRPLF"
BASE COUNT         1792 a         1824 c         1729 g         1863 t
ORIGIN      
        1 gcacaccatc acccatcgct cgtttgataa cgctcacttc ctgactcaac acttctggcc
       61 gattccggag aagcctatgg tcattggccg ccattatcca atcagacaat tcgcggaacg
      121 catcaagtcc tatccagatc acggcatctt gtgcttggca attaaactga tctgtttctg
      181 gcattcgatt ctctcctcga gcaaagaaag agtttgtttt agtcggctac ccttcagctt
      241 ctcccaactg tgcaatgcga ggcggatgag actgatcaat tcataaattg caacccgccc
      301 cctaaagact tcctcatcag tgatccagcc ttcagaggca ttaatatatg catccaagaa
      361 aatggcacat aaatgctcgc tggcttcggg agaaagcggg gaatttggat cccgaaccct
      421 aacgatactt agcctttgat acgctaggta ttgccccaga tcctgtgctg gctccgcctg
      481 gcaaatggaa tcaaaatcga ccaggccgcc atttgttccg tcaaagatca actgagtgta
      541 tctgaagtca ccgtgactaa agcataatgg catggctgga taggcttgcg caaattcgac
      601 ggtttggtca atccaggatc tgacctccgc accgacttca ggaagggcct ggcacaaaac
      661 gtccacctct tccaataaat tagctacctg cgtctccaat gtgttttgca atcccaactt
      721 aatattggtt ccatgaagaa tggcggcaat ccacgcgcaa gccctgatgg cctgttccag
      781 cgccacatcc ctctcggtcg aaggttttcc atctctagta ccagtccatg ttttcaacaa
      841 ttccttgaag aaaggttttc ccggcaaagc ttccatcagg agtaacttta agtctgggag
      901 ataacctaac gaatgtggga cgcggaatcg atatggtaga tcaggttttt gtaaatactc
      961 tcttaataga gacaggatcg gtccgatctg ctctcccaat ccattcgcgt ctatttttcc
     1021 atacacagtt acattttgtg gggtttgtgt ttccgtttgg ataccatcca cagaatatcg
     1081 caaaacacaa cgtttatagc gcccgtaatg tgcagggagc aatcgcacat ctttaattac
     1141 aaattcaccc gacaacgctt ctggcaaagc ttcttctaaa atactcgcca tcttagctgg
     1201 atctgtcgcg tctagtagag tgggaatcag accatcaatg ggaaagacgg aaagagccat
     1261 atgtagatgt tcgatgatcg cgacaggtct ggcaaatggc ttgatttccg gtcgctctgt
     1321 catcctcgca gcacttggca tcagtatttt ttccaggtaa gttgtgcact ccaccaggtc
     1381 cgggaaaagc tgcgcattaa tcagcgtttc cgcggtctgc ttattgatgt gatgctgaat
     1441 ggtcagcttg tattgcatat tgatgatgtg gtccggcagg tagagtgcct ttccaggaac
     1501 acatttaagg atcgaatact ggctcggatt agagtttaga agcgcctctt gcaatacggg
     1561 agtcatccgc tcgacgtcaa aggcttccat cagcgccggg gccttctctc catacagcgt
     1621 gctgcgtggc agatcatcat tgatgttttc aatcaattct tgaatcgccg ggtttgccat
     1681 acctgtcttc aaccttttcc cagctttgga tctgccgtct cggagatagg aagagggtta
     1741 ttctccattg ctctttgact ggattgcatg atgtacagat tggcatacaa accacccgcg
     1801 cggatcagct catcatgcgt gccgatctgc tcgatttgtc cttctttgat gacgatgatc
     1861 tgatctgcat tgcgaatcaa attcagctca tgcgagataa taatggtggt cttgtccttc
     1921 atcaatttac gtaatgcctg caggaccagg tctgttgatt ctgcatccaa cccggtggag
     1981 ggctcatcca ggatcagaat aggtgtgtca cggataaagg ctcgggctat cgcgatccgc
     2041 tggcgctgac cgccagataa gttcgacccg cgctccgcca gtacagtgta atacccatct
     2101 gaaaacttgg agatgaattc atgggcattg gcatccttgg ccgcctcgat gatttcgatc
     2161 cccgtggcat ccgggcgtcc atatccaata ttctccaaga tgctgcccgt gaataagatg
     2221 gattcctgga gcaccatgct catctgtgcg cgcaacgatt ccagactgta gtcgcggata
     2281 tcatgcccat cgatcaatat ttgtccttca tccggatcat agaggcgagg aagcagttgt
     2341 gcgacggtgc ttttccctgc tccggtatgc cctaccaacg ccacaacctg gcctggccga
     2401 acgtcaaagg aaaggccgtt taatgtagga cgtgcttttc cttctccttt tttcttcgaa
     2461 gtttcgacgc tcgggtagga aaagacaaca tttcgaaact caattcgccc atgaaaagac
     2521 ggtgccgtaa gtgcgtctgg cttatcgtgt acctcaggcg tcaaatctaa aagatcaccg
     2581 atccgttcca gactggcata cagcttgcca aacgcgttcc actgggagat cagtttcttc
     2641 gcgggcttga acatattatc tacccagacg atgaatgtaa gcaagatacc aggttggatg
     2701 ctgcccggat ttgcgcgtat gatcccgact ccgatccaga tgataatagc ggtaacgacg
     2761 gcagccagaa cacacattgc ccatttaaag cgtgcctggt acgccgcagc ctcgagggca
     2821 gcattcatcg ctttctggct ctgtccggaa aagagggatt gttcgtagtc tccctgacca
     2881 tatacctgta ccactcggat cgaggctaac atttcctgcg ccgcggaagc cagctcacct
     2941 tcggtggagc gcagccgttt gctcgccgat ttgatccttc cggtgaaata attcgtgatc
     3001 aaggccatca ctgggatgat cgccagagcg accagcggaa taataaacac agtaggtgtt
     3061 ccaactgtcc ccacaacttc ctcaggtggc tttaggttca agcacacatt tgcgaacgtg
     3121 gatgggatcc atctgttgca ggaatcctga agaagtgagg gtctccagac cgtcacagcg
     3181 cccaggcaca tagcaaacat aagaataaca atgttcaata tgctgcccag gaaatccgaa
     3241 agattagaaa tgatgaagtc ttccagagct gccacatcgg aggttaccct gctgagaata
     3301 tcgcctgtgc ggctctgtcc atggaaggag agtgaaagtt tttgcaggtg gttatacagg
     3361 aaaacgcgca tattgtaacc aaccttacga ccgccctggg cgaggtagat cgcagacaag
     3421 gagtcgcaca ggctgttgag catggtcagg atgatcaggc caagcgtaat gaagatcagg
     3481 gttgtcacga cggaattaac atcaagtgca gccaggctct ctgccgccaa atttacaaca
     3541 gcacctggca ccaaaagggg aatcaatact gccgtagccg actcgagaat caacatgacc
     3601 agggagagta tgaagatccg ccgtgaattg cccgaaaatc gcgtaatcat tcccactaat
     3661 gggcgtatat tgagtccgct cgaagaacct ttttggtcgt cagaagattt tttactcata
     3721 gctcaccaac ttaatcataa gaataaaggt cataatttca cgcaaacaag ttttgtaagc
     3781 gatttgccgc cagggcaatg tcaaattgtt cattcacttt tttggcgcct gcattaccca
     3841 actgtcgccg tttttccgcg tcgcgtaaaa gctcgagaat ccccgaggaa ataccattaa
     3901 catcgtgtgg ttggtataaa agcccgttca ggttgtcatc taccagttcc ggaatcccgg
     3961 ctacggctgt agtaactacg ggcagtccca cagccatagc ctcgaccagc acatttggga
     4021 taccgtcccg gtcgccatct tctgtttgca tcggcgtgag cgcaaagatg gtggcgtttt
     4081 gatagatgga aatcaactcc tgctgggtga tgtcgcccat cagccggacg tcgtctgcca
     4141 tgtcatgttc ctcaatccat tgctctaact tctcagaaag aggtccacct ccgtaaatcg
     4201 tgcattggaa ttgcagtccg ccttctttga ccataagcaa tgcctgcaat aaatcgagga
     4261 accccttctt ctccacgagt cgccctaccg acaagataat gggaacctct gacgtggaat
     4321 ttgcgccatt gctggaggat gctcgaaagt cgtcgagatt cacaccgtga tagataagcg
     4381 aaaattttga ctgctctgac ggcgcaacct ggttcaggta atccatgttt acacgacaac
     4441 acgtgatcac accctgggat gttcggattc gatctgttaa aatttgctcg ggtatctgat
     4501 aaagatcacg ggcatgcgcg gtaaaactat agggaagacc ggtcatgagg tttgccagat
     4561 aggcgattaa tgtggggtca tgcgcaaaat gggcatgcag atgatcgatc tttttcccgg
     4621 tttctcgttc ctgcacgcgg agcacatgaa tcagatgaac agcttgatga aagcattccc
     4681 aacggctact ggcggtatat ccttgatcaa taactggatg catcacaacg tataacaggg
     4741 aacggaagta tcccgtgaaa tggcgcattg atacttccat gtgttccttg agcatatttc
     4801 cgaaccggcg cgattgtatc gattcatcca gataatggac cggagcctga acctgatcga
     4861 cttgcatttg tacaaccttt tcatgcgggt tggtcagggc aaagatctgg atcgaaaccc
     4921 cgatcttttc caaagcaaga atctcgttca gaataaaggt ctgtgagaga cgaggatagc
     4981 tccggaggag gtacccgatt tgcttatttg ataggctttc gaccatcttg tcgccttctt
     5041 tatttctgca aattcataaa caggaacttc gatgttcttt tgcgaggccc tggtcatagc
     5101 gctctcgccc attatcaagc cttgttcgat cattaaccag acctgcgcct gttgttcatc
     5161 tccttccggc cagggacgcg ggccacggaa tttgcataat tgcttggcta ttttgagaca
     5221 ggagtagatg tagaagaaca ggaatttctc gtcccattgc catcctgttc cactggaata
     5281 agcatctaaa aataggtttt ggagatctgt aaacttcgcc gtatgttcgt tattacggta
     5341 tgccagcagg tagaaattgg cgcaaaaatg agctagatca aaattcggat cgcccaggcg
     5401 catttcatca aaatcaaaca ccttgaggtt taagccctca tcgatcagaa tatgttcgta
     5461 atgaaaatcc ttatggatgg gggcatgagc gctaaagtca agctcggtcg tcctttggac
     5521 cagatactcg gcaatatgag tagctgcctg tctctccttt ggatatttct gggagatcag
     5581 ctcagcccag tcgcgtatgt tatcgatttc attttcaacc ttgaattcct tttcgagcgg
     5641 gaatcggtgt gtgtgcaatt gcgccagcca ttttccggaa agctgcatag cgcggatcac
     5701 atcatcgccg tctaatgagc gtttcgcgat gtaatcgccg agcaacttac ctcccgccgg
     5761 aataaaaacg agcatcgata aatcggggat cacccctaat ggttccggca cacctaaatc
     5821 tgtggcatca cgaaatactt catcgtgcag cgttttcaag accttataag ctcgttccga
     5881 taaatgcggt tccggataga gtttgccgaa gacttcatca tgtttttggc actcgatctg
     5941 gtccaggtca taacgaatga caccacgctt ccccggttta tacgctaaga gctttacccg
     6001 gtggacattt cgcgaggaag tagcagaaca tatgtcactg agcataggtg tgagattact
     6061 attcatgatt tcggcattga gtgcgctcca caatgccgga aatggcacat cctgaaacgt
     6121 atgtggaaat ttcgttttgc cggtagctgt ccggagatga gcgaccagtg tccctttgag
     6181 gtcagtgatt gtatgtgcct ccaagtttgc taagtcttct gtaattgcag aatgggctgc
     6241 ttgaatcagg tgaataatga gcggccattc gagataggtc aggcttcgaa accgacgtcc
     6301 ggcgattttc agcaatgagg ctgcctggta gaccgataac caccgttcat cgatatctgc
     6361 atgcacgctc aagtatcctt ccaggaatgc ccgacccgca cgttcgacaa actgggcatg
     6421 ttctggttgg cggattgcct tccaacacaa gtacgccagg aaattcccca gatcccgcgc
     6481 cgggttggcc caacagtaac tatccagatc gatcatcgcc agacgatctc cttgtacgat
     6541 gtactgatcc gtgcgcatcg ccccgtggct ggctactggc ttcggttctt gaaactgact
     6601 tactttggtg ttaatgtgtt gaatgacctc ttcatatttg gccgccaagt ccgcccggac
     6661 cttgcctatt atcacggtat attcatgcag gtcttgcaga tcgtcgtaca cggttttcgt
     6721 ttcaccggga acggtcgaat tgtcctggaa gacccccaag gcacgacctg ctttcacgaa
     6781 ccaatcttca cggagtgaag cgtcataggc ctggtcgtaa gcaaaatggg aaaattcaat
     6841 gccgttagga accgcggctt gtacgatcat ctccaggtcc ggccagaccg ctaccggcgc
     6901 ggagattagt ggcatctccg gattttcctc actgcacgta tgcaagtcta tgattgtctt
     6961 atacagctgt tctgcatctc cggtaaatga tttcccataa aacattttgt taccagaagg
     7021 tgttcgcgcg gtataacgga taacacaatg caggccgggt ttatagcgaa cagggaagat
     7081 ctcccagagc cttacctgat cgccaaagcc tgcaaaggcg gggagctctg aaaagcgtcg
     7141 ctgcatttca ttcaaatccg ttgccagggc caaccacgga agtcgttcgt ccgcggtaaa
     7201 aaggcgtg
//
LOCUS       DASTJP010000365         5421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1439241, whole genome shotgun sequence.
ACCESSION   DASTJP010000365 DASTJP010000000
VERSION     DASTJP010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439241"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFQ23_10810"
     CDS             <1..479
                     /locus_tag="VFQ23_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9907126.1"
                     /translation="IVLKTAKYKFPNSLIFVFSRNPNEREFALSLGAVWAGAIDDSPP
                     QRLDAIIDTTPVWRPISAALRYLNPGGRLVINAIRKEESDKEVLLKLDYPSQLWMEKE
                     IKSVANVTRADVREFLHLAAEANIRPEFQEYDLQNANQALLEMKQGKIRGAKVLRI"
     gene            476..772
                     /locus_tag="VFQ23_10815"
     CDS             476..772
                     /locus_tag="VFQ23_10815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907127.1"
                     /translation="MTIQRDPEPKEIKYLHKFADFSEKRVLEIGCGEGRLTWQYARKS
                     KITIGFDVDADALRVASIERPSDLQSKVLFSRAESEYLPFHKETFDMALLSWSF"
     gene            827..1210
                     /locus_tag="VFQ23_10820"
     CDS             827..1210
                     /locus_tag="VFQ23_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907128.1"
                     /translation="MPDGVLIDLRPMLDRWQVQVVSARQVQDTGRLQDFPTGLADDNA
                     ANQAMAKAERDGWFTRENEEFFLIDYSWDTPREMEEWIKDEWHDFIDLDEEARRFTRS
                     AWAVGDADSLVRVRVKMLITRWRKT"
     gene            1267..2370
                     /locus_tag="VFQ23_10825"
     CDS             1267..2370
                     /locus_tag="VFQ23_10825"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9907129.1"
                     /translation="MSESSKITRRNFLQILLAAGAGGLTAGVLRALNSSAPISTPIKN
                     IIVFIQENHSFDSIFADFPGANGMYAGKDCPDALSTDPPHKHSDAFAPQGATTDEARC
                     SYAEADGPNYWEMARSFTLCDNYFADVRGPSHPNFLMLIAAQSPIIEPPRPDVCPDFC
                     LDIEVLPHRLDAAGLTWQDYGGIFTSTKSLVGRPEVMDYRDAKFFVDAAEGTLPNVGW
                     LNSSFLYEGYPKSGHPPSGLCEGENYAVEVLNAVMNSPQWESTALFLVWDEWGGFYDH
                     VEPPVVEHWTDGRASRYGHRVPCLVISPYARPGHIAHTLYSHVSILRFAETVLDLEPL
                     TERDANADNMFDCFDFDQTPLPPISLVPRDCPT"
     gene            complement(2367..3542)
                     /locus_tag="VFQ23_10830"
     CDS             complement(2367..3542)
                     /locus_tag="VFQ23_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907130.1"
                     /translation="MTKIQLRNVNLTYPAPLQDPANRIFKIGHDRLQEDLPLETEAGV
                     RALDHVNLKIPNGRTFVIVGPSGCGKSTLLRVIAGLINDYSGEVMYDEEDMRNIPPKD
                     RYIGMVFQNYALYPNFKNEGNLSFFFKMHKITDEETYERIRYTSELMGVGFKDLLPRK
                     PGSLSGGERQRVAIARAIVRAPRLLLFDEPLSNLDAKLRVQTRTEIKRLLRRFSITTL
                     YVTHDQVEAIALADQIIVMHEGRIEQTGTYQNLIENPINMFVAGFFGLPTMNLFSGGF
                     VSGTHLTLGDNSVALPERVLPLVQIDQSVTLGIRPEAVTVTTEHQPTNEIQLSGEVEA
                     LESDFVHHTQLVHIRTAQWTYWGYCSLNLSLQIGQRVYAMLDAERLYFFDTVSGTRI"
     gene            3651..4208
                     /locus_tag="VFQ23_10835"
     CDS             3651..4208
                     /locus_tag="VFQ23_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET9907131.1"
                     /translation="MLNTQHLSMVELETGMDHIRNSPKDDGVLKLIVRRPSVDAREVI
                     YAGQLNPTDGLEGDTWKTRGSSHTADGSANISAQITVMNSRAIALLAQDEERWSLAGD
                     QLYVDMDLSDENIPAGTQLRIGSAIIEVSAQPHSGCKKFSSRFGVEAMKFVNSPEGKR
                     LHLRGINARVVQAGTIRVGDIIEKI"
     gene            4237..5058
                     /locus_tag="VFQ23_10840"
     CDS             4237..5058
                     /locus_tag="VFQ23_10840"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HET9907132.1"
                     /translation="MRVNPNHQLILDRFITACQTDDRIAAVLLLGSYVKGMADEHSDL
                     DLYVITTDEGYEDCTANRSAFVESLGEPLFAENFDIPDFVFLIFADGSEVEISFARES
                     QLSQVLSASYKVLLDKRNITAYISLHTPTVDPEAQTEKLRRLIHWFWHELSHFTTALA
                     RKELWWAQGQLGALRLCCINLTRLESDFFDQEIGEEGYFKIEKAIPIQRLSALNSTYC
                     PMEEGAMLESAFIIVQFFRDLAPGLASKHGINYPEQLDKVIVQKLGNVKRLNTNG"
     gene            5051..>5421
                     /locus_tag="VFQ23_10845"
     CDS             5051..>5421
                     /locus_tag="VFQ23_10845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907133.1"
                     /translation="MDNQSSNAADFHSQTDDVFGRIATRYDLLCDLFSLGIHRLWKRR
                     VANLIAQEPWNCLLDAASGTGDVVLRLVKQQPLRPEQIIITSDISPQMLAVAKQRAGP
                     FGSSLDFRVLDANSMPEIPNA"
BASE COUNT         1364 a         1296 c         1427 g         1334 t
ORIGIN      
        1 acatcgtatt gaaaactgca aagtacaagt tccccaactc tctaattttt gtgtttagta
       61 gaaatccgaa tgagcgagaa ttcgcgcttt cgttgggagc ggtctgggca ggcgcgattg
      121 atgacagtcc acctcaaaga ttggatgcga tcattgacac gacaccggtt tggcgtccaa
      181 ttagtgcagc gttgaggtat ctaaatccgg gtggaaggct cgtcatcaat gccatccgca
      241 aagaggaatc tgacaaagag gttctactga agctggatta tccatctcag ttatggatgg
      301 agaaggaaat caaatctgtt gccaatgtga cccgggcaga cgtgcgcgag tttctgcact
      361 tggcagcaga ggcaaacatc cgacctgaat ttcaggaata cgatctgcag aatgcaaatc
      421 aggcattgct ggaaatgaag caaggtaaaa ttcggggagc aaaagtgctg cggatatgac
      481 catccaacga gaccctgaac ccaaagaaat taaatatctt cacaagtttg cagacttcag
      541 cgaaaagcgt gtgctggaga tcggctgcgg cgaaggacga ttgacctggc aatacgcacg
      601 caagtcaaaa atcactattg ggttcgatgt agacgccgat gcgttgcgcg ttgcatccat
      661 cgagcgccct tcagacttgc agagtaaagt gctcttttca agagcagagt cagaatattt
      721 gccatttcac aaagaaacgt ttgatatggc attgctctcc tggtccttct gatgaattgc
      781 gcatgcgggc atggttcatg ccctaagcga aattcgaaga gtgcttgtgc ctgatggagt
      841 cctgatcgat ttgcgtccca tgctggatcg ctggcaggtt caagttgtat cggctcggca
      901 agttcaggat acgggccggt tgcaggattt tccgactggt cttgcggatg ataacgctgc
      961 aaatcaagcc atggcgaaag ccgaacgaga tggctggttc actcgtgaaa atgaggagtt
     1021 cttcctgatc gattattcgt gggacacgcc ccgcgaaatg gaagaatgga tcaaggatga
     1081 atggcatgat tttattgatc ttgacgaaga agcgcggcga ttcacccgct ctgcatgggc
     1141 agtgggtgat gcagacagcc tggtccgtgt aagagtgaag atgctgatca cgcgctggcg
     1201 gaaaacataa tttcgttgat ccgaaggccg aagtgaggtt tgcggcgagg attcccgctt
     1261 aattaaatgt ccgagtcttc gaaaattact cgccggaatt ttcttcaaat cctgctggca
     1321 gccggcgcgg gtggacttac cgcaggcgta ttgcgcgcac tcaattcatc tgcgccgatc
     1381 tccactccga ttaaaaacat catcgttttc atacaagaga atcattcgtt tgatagcatc
     1441 ttcgcagatt tccccggtgc aaatgggatg tacgccggaa aagattgtcc ggatgccctg
     1501 tccacagatc ctcctcacaa acatagcgac gcctttgctc cacaaggggc aacgacggac
     1561 gaggcgagat gctcctatgc ggaagcggac gggccaaatt actgggaaat ggctcgctcg
     1621 tttactttat gcgataacta ctttgcggat gtgcgcggtc catcccaccc taactttttg
     1681 atgctcatcg cagcgcaatc gcctatcata gagccgccac gtcctgatgt gtgcccggat
     1741 ttttgtctgg atattgaagt tttgccacat cgtctggatg cagcgggact cacctggcag
     1801 gattatggag gaatatttac gagcacaaag agcctggtcg gtcgccccga agtgatggat
     1861 tatcgcgatg cgaaattctt tgtggatgcc gccgagggaa cgctgccaaa tgtcggctgg
     1921 ttaaattcaa gctttttgta tgaaggatat ccaaaaagtg gtcacccacc ctccggcttg
     1981 tgtgagggag aaaattatgc agtagaggtt ttgaatgcgg tgatgaatag tccacaatgg
     2041 gaatcaaccg cgctctttct cgtatgggat gaatggggcg ggttttacga tcacgtggaa
     2101 ccacccgtcg tggagcactg gaccgatggg agagcgtcgc gttacgggca tcgcgtgcca
     2161 tgtctggtga tcagtccgta tgcgcggccg ggtcatattg cacatacact ttactcacat
     2221 gtgagtattc tgcgctttgc tgaaacggtc ctcgacctgg agcccctcac cgagcgtgac
     2281 gcgaacgcgg ataacatgtt tgattgtttt gatttcgatc agactccgtt accaccaatt
     2341 tctctcgtgc cacgggactg tcccacttag attcgagtgc ctgatacagt gtcgaagaaa
     2401 tacaaacgct cagcgtccaa catggcgtag actctttgcc cgatttggag actaagattc
     2461 agcgagcaat atccccagta ggtccactgt gcagtccgga tgtgaacgag ctgcgtatga
     2521 tgcacgaaat ctgattcaag cgcttcgacc tcaccagaga gctggatctc gtttgtaggc
     2581 tgatgctcag tggtgaccgt caccgcttcg gggcgaatcc ccagggtcac agactggtcg
     2641 atctggacaa gtggaagaac acgttcaggg agcgcaaccg aattgtcgcc cagggtcaga
     2701 tgcgtcccgg aaacgaatcc gcctgaaaac aaattcattg tgggtagccc gaaaaaccca
     2761 gcgacaaaca tattaatcgg attttcgatc aggttctgat aagtaccggt ttgctcgatc
     2821 ctcccctcgt gcatcacaat aatctgatca gccagtgcta tcgcctccac ttgatcgtgc
     2881 gtgacgtaaa gggttgtgat gctaaagcgc cgcaacaacc gtttgatctc ggtgcgggtt
     2941 tgtacgcgta gtttcgcatc caggttggaa agtggctcat caaagagaag cagccgcggc
     3001 gcacggacga ttgcacgggc aatagcaact cgctggcgtt ccccacccga gagggagccg
     3061 ggcttgcgcg gtagtaagtc cttaaaacca acgcccatta actctgatgt gtaacggatg
     3121 cgctcatagg tctcttcgtc ggtgatctta tgcattttga agaagaacga gagattccct
     3181 tcgtttttga agtttggata gagcgcataa ttttgaaaga ccatgccgat gtaacgatct
     3241 ttcggtggaa tgttgcgcat atcttcctcg tcatacatca cttctccgga gtaatcattg
     3301 atcagcccgg caatcactcg cagcagcgta cttttcccgc acccggaagg tcccacgatg
     3361 acaaaggttc gcccgttggg aattttcaaa ttcacgtgat cgagcgcgcg gactcctgcc
     3421 tctgtttcca ggggcaggtc ctcctgcagc cgatcgtgtc cgatcttaaa aatacgattt
     3481 gcgggatcct gcagtggagc gggataggtc aaatttacat ttcgcagttg aatttttgtc
     3541 ataatctgat ttttgtccga taagatttta tcattggcga ctgctggcgg atggaaaatc
     3601 acctgagccg aggcttcaaa tgctatactt gaagtagaaa ggaaaccagg atgttaaaca
     3661 cacagcactt atcaatggta gaacttgaga ccggcatgga tcacattcgc aattcgccaa
     3721 aagacgatgg cgtgttgaaa ttgattgtgc gacgtcccag tgttgacgcg cgcgaagtca
     3781 tctatgcagg tcaactcaat ccaaccgacg gactggaagg ggacacatgg aaaacgcgcg
     3841 gcagcagcca tacagccgat ggatctgcaa atatcagcgc tcaaatcaca gtcatgaatt
     3901 cgcgtgcgat tgccctgctg gcgcaggatg aagagcgctg gtcacttgcg ggagatcagc
     3961 tctacgtcga catggatctg agcgacgaga atatccctgc cggcacacag ttgaggatcg
     4021 gttcagccat cattgaagtc tctgcgcagc cacattctgg ctgcaagaag ttttcgtcgc
     4081 gcttcggggt ggaagcgatg aagtttgtca attcgcccga gggaaagcga ttacatctgc
     4141 gcggtattaa tgccagagtg gttcaggccg gaacgattcg ggttggggat attatagaga
     4201 aaatctaaaa aaaactccga cagaaggcag gtagttatgc gagtcaatcc aaatcatcag
     4261 ctcattttgg atcgtttcat cacagcctgc cagactgatg atcgtatcgc agctgtcctg
     4321 cttttggggt cctatgtgaa aggaatggct gatgaacatt ctgacctgga tctgtatgta
     4381 atcaccacag acgagggcta tgaggactgc accgccaaca ggtctgcatt tgtagaaagc
     4441 ctcggcgaac cactgtttgc agaaaacttc gatattccgg acttcgtgtt cctgatcttt
     4501 gcagatggct cggaagttga aatctcattc gctcgtgaaa gccagcttag ccaggttctt
     4561 agtgcatctt ataaagttct tttggataag cggaatatca ccgcctatat ttccttacac
     4621 actcctaccg ttgacccgga agcacaaacc gaaaaactgc gccgcctcat ccattggttt
     4681 tggcatgagc tatctcattt caccaccgcg ctggcacgta aggaactctg gtgggcacag
     4741 ggtcaacttg gggcactacg gctttgttgc atcaatctaa ctcgactaga aagtgacttc
     4801 tttgatcagg agatcggtga agaaggctat ttcaagattg agaaagctat ccccatccaa
     4861 cgattgtctg cgctgaactc cacctattgc ccgatggagg agggcgcaat gttggagtct
     4921 gcctttatca ttgtgcaatt ctttcgggat ctggcgccag ggcttgcctc aaagcatgga
     4981 attaattatc cggaacaact tgacaaagta attgtccaaa aactggggaa tgtaaaacga
     5041 ttgaacacga atggataatc aatcgtcgaa tgccgcggat tttcatagtc agacagatga
     5101 tgttttcggt cgaatcgcca cgcgctacga tttgctgtgt gacttattta gtctcggcat
     5161 tcatcgactc tggaagagaa gggtcgcaaa ccttatcgca caagagccat ggaactgctt
     5221 gctggatgcc gcgtcaggca cgggggatgt ggtcttgcga ttggtgaaac agcaaccctt
     5281 aagacccgag caaatcatca ttacttcaga catcagccct caaatgctgg cggttgctaa
     5341 gcaaagagcc ggaccgtttg ggtcatctct cgattttcgt gttctggacg ccaactcgat
     5401 gccggaaatc cctaacgcca g
//
LOCUS       DASTJP010000366         5343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_10820, whole genome shotgun sequence.
ACCESSION   DASTJP010000366 DASTJP010000000
VERSION     DASTJP010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10820"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VFQ23_10850"
     CDS             complement(<1..1142)
                     /locus_tag="VFQ23_10850"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9907134.1"
                     /translation="MASSQQAAPNDRREIFGWAMYDWANSAFSTTVGSVFLGLYVAAL
                     AREAAAASSDGVARFLGLPVAPTAVIPFAISLSVVFQAGLLPILGAIADYSHRRKQML
                     QLFATIGAIATASLFFVTQNTWQLGPLLFIIANLSFGAALVFYNAYLPDIATEEERDR
                     VSSYGFALGYLGGFILLALNLALFLLHENFGITESMSVRINLASAGLWWLGFSTITWS
                     RLRSRHGARSMPTGENLFSIAFKQLSETMEASPRLIAFLLLSPLLIFLWAPLITPYVT
                     RLIEINQLDPDLATLPIFIPMFGPVIMLIIFLRNKYRTVPETSKFLTSYLIYNDGIQT
                     VIAVASTFAAAPLIEGGLELTQDTLIAVILMIQFVAFGGALFWGKLA"
     gene            1386..5282
                     /gene="rpoB"
                     /locus_tag="VFQ23_10855"
     CDS             1386..5282
                     /gene="rpoB"
                     /locus_tag="VFQ23_10855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9907135.1"
                     /translation="MSSSLPRKTYARIPVNIKLPNLIEVQLDSFERLKREGLGDLFHE
                     VSPIESYNKGMKLYFPSRSPESKQWGLKYWFGEPKHTIEECVERDLTYSSPLYVSVLL
                     AGPDVPEPIKQDIFLGDFPEMTDKGTFIINGTERVVVSQLIRSPGVYFEAPTDRATGR
                     SLAMSKLIPDRGAWMEFETRKSDYIILKFNRKRTVPITVFLRALSAVDDGIKNSMLTT
                     GSDEEILSLFQDVDNNPERLFIASTLKQEPEWEITSKQTIAEAALIEFFRKMRPGDPA
                     TLDNARQFLEEQLFDQRHYDLERVGRYKLNQKLDLDIPIPHRTVSKGDVIRLVRRMIQ
                     INNGVEPPDDIDHLGNRRVKTVGELIQNKLRIGLRRMERVIKERMSIRDQEQLSPVTL
                     VNIRPVVAALREFFGSSQLSQFMDQTNPLAELRHKRTLSALGPGGLRRERAGFDVRDV
                     HHSHYGRICPIETPEGPNIGLIGRLASFSRVNEYGFIETPYRKVFRVMQADDERLEGR
                     TLRSNVVDPKSEEVLFKAGELIDAKILKRISKTGSPVDIVPYVSDQFDYLSADAEDKY
                     VIAQANAPLTENKQFVRERVSCRYHSGFIFSTPDAVDYMDVAPHQVVGISAALIPFLE
                     HDDANRALMGSNMQAQAVPLVRPEIPLVSTGMEYHAALDSGQVVVAEADGEITSVTGN
                     SITVKERGGIRNYVLRKYQRSNQSTCIDQRPAVVKGQRIKKGDIIADSSSTESGQLAL
                     GQNVVVAFLSWEGGNFEDAILISERLVQEDRFTSVHIEKYEVEARDTKLGPEEITRDI
                     PNVGEDAIKDLDENGIIRIGAEVGPNDILVGKITPKGEKELTPEERLLRAIFGEKSRD
                     VKDTSLRMPHGERGKVVDVKVFTREENSDLSAGVDMMVRVSVAQRRKLTAGDKMAGRH
                     GNKGVVSKVVPVEDMPFLEDGTPVDIILNPLGVPGRMNIGQVLEVHLGWAAKRLGYRA
                     VTPVFDGASEEEIEAELARAWIIDQAWKETAATSWEWIKQQEYDPNSIQDDDEVRRLY
                     LEEWLGERDYDPYDLNDVDYARHATASEWLRERGYDPDTIFHPDDADPRSRESYNAAA
                     ISACLRLWIESHGHGGRIAEARVAEVAQELTSSTGHPLPILGKQTLRDGKSGIPYDQP
                     VTVGVMTILKLHHLVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYG
                     AAYTLQEMLTVKSDDVQGRVNTYEAIVKGEPIEEPSIPASFRVLVKELQSLGLAVEAI
                     NEGGDVVKFGKDEEKQHPPKLNTGLLGIGDNLMTKQ"
BASE COUNT         1399 a         1221 c         1395 g         1328 t
ORIGIN      
        1 gcaagtttgc cccagaatag agccccgcca aaagccacga attggatcat taagataact
       61 gcgattagtg tgtcttgcgt aagctcaagc cctccttcta ttagtggggc ggctgcaaag
      121 gtggatgcta ccgctatgac cgtttgaata ccatcgttat atatcaagta tgaggtgaga
      181 aatttcgagg tttctggaac tgttctatat ttgttgcgga ggaaaataat caacatgatc
      241 acgggcccga acattgggat gaaaattggc agagtggcaa gatccgggtc taattgattt
      301 atctctatta gtcttgttac atagggggta atcaaaggag cccataaaaa tatgagcaga
      361 ggcgaaagca aaaggaacgc aatcaacctt ggtgatgctt ccatcgtctc cgaaagttgc
      421 ttgaaagcaa tgctaaataa attttctcca gtcggcatgg aacgggctcc gtgccgtgaa
      481 cgaagcctgc tccatgttat tgtggaaaac ccaagccacc ataaacctgc tgaggccaga
      541 ttgatgcgga cagacatact ctcggtaatt ccaaaatttt catgtagtaa gaataacgct
      601 agatttagcg cgagcagtat gaatcctcca agataaccta atgcgaagcc atacgaagag
      661 acacggtcgc gctcttcttc ggtcgcaatg tctggcaagt aggcattgta aaagacaagc
      721 gccgcgccaa aggaaaggtt agcaataatg aataatagag gtccaagttg ccatgtattc
      781 tgagttacga agaacaatga agcagtcgct attgcgccga ttgtggcgaa taattgcaac
      841 atttgcttgc ggcggtgaga ataatcagcg atcgcgccaa gaatcggcaa gagaccagcc
      901 tggaagacga ctgacaagga aattgcaaat ggaataacag cagtaggagc tacgggaaga
      961 ccaagaaacc gcgctacccc atcgctggaa gcagctgcag cttctctagc tagtgctgcc
     1021 acgtaaagcc caagaaagac tgatccaact gttgtgctga aggctgagtt tgcccaatca
     1081 tacattgccc agccgaaaat ttcacgtcga tcgtttggtg ccgcctgctg agatgaagcc
     1141 atattgcctc ccgcacgttg ggatagtatt acttgtctaa accccgtttg gttctctcaa
     1201 gttgcgaaaa tttggaattg tgatagaatc ttgttaacat tgaatgtgaa ggggtaaaat
     1261 cagttgaaaa tcccttgcgt atatataaac attgatatat aatcatcgtt tgctatcccg
     1321 ctaaaacagc gggatgggtt tgtgtcgtta aacgctgaat aggtcccagg tcaggagtaa
     1381 accgaatgtc ttcttcttta ccacgtaaaa cttacgcgcg tattcccgtt aatattaaac
     1441 tacccaatct gatcgaagtc cagctggatt catttgagcg cctaaagcgc gagggactcg
     1501 gcgatctctt ccacgaggtt tcaccgatcg agtcctataa caaggggatg aaattatatt
     1561 tcccgagccg cagccccgag tcaaagcaat ggggtttgaa gtactggttt ggtgaaccca
     1621 aacatacaat tgaagaatgt gtcgaacgtg acttaacata ttccagccct ctttatgtct
     1681 ctgtattgct ggctggacct gatgtacccg agccaatcaa gcaggatatc ttcctgggcg
     1741 acttcccgga gatgacggat aaaggcacgt tcatcattaa tggaaccgaa cgtgttgtcg
     1801 tttcacaatt gatccgttcg ccgggcgttt actttgaggc gcccaccgat cgcgccaccg
     1861 ggcgctcgct tgccatgtcc aagctgatcc ccgatcgcgg cgcgtggatg gaatttgaaa
     1921 cgcgtaaatc tgattacatc attttgaagt tcaatcgcaa gcgcacggtg ccgatcacag
     1981 tattcctgcg tgcactctca gcagtggatg atggcataaa gaactctatg ctcactactg
     2041 gtagtgatga agaaatttta tctttgtttc aggatgtaga taataatcct gaacgtttat
     2101 tcattgcttc aacacttaag caggaacccg aatgggaaat tacaagcaag cagacaattg
     2161 ctgaggctgc attgatcgag tttttccgca agatgcgccc tggcgatccc gcaacacttg
     2221 ataatgcccg tcaattcctc gaagaacaac tcttcgatca gcgtcactat gacctcgaac
     2281 gcgtgggtcg ttacaaatta aatcagaaac tggatctgga tatccccatc ccgcaccgaa
     2341 cggtttccaa gggcgatgtg attcgactcg tccgacgcat gattcaaatc aataacggag
     2401 tcgaaccacc ggatgatatc gatcatcttg gaaaccgacg tgtcaagacg gtgggcgagc
     2461 tgattcagaa caagctgcga atcggcctgc gacgcatgga gcgcgtgatc aaagagcgca
     2521 tgtccattcg tgatcaggaa cagctctcac cggtcacgct ggtcaacatt cgtcctgtcg
     2581 ttgcagcatt gcgtgagttt tttggttcat cccagctttc gcaattcatg gatcagacca
     2641 atccgttggc tgaactacgc cacaaacgaa ccctgtctgc tctcggtccg ggtggtttgc
     2701 gtcgtgagcg cgccggcttc gacgtgcgcg atgttcacca ctctcactac ggacgcattt
     2761 gtccgattga aacaccggaa ggtccaaaca tcggtttgat cggtcgcctg gcttctttct
     2821 ctcgtgtaaa cgaatatggt ttcatcgaga cgccgtatcg aaaagtattt cgagtcatgc
     2881 aagccgatga tgagcgtctt gaaggtcgca cgcttcggtc aaatgttgtt gaccccaagt
     2941 ctgaagaagt attgttcaaa gccggtgaat taattgatgc gaagatattg aagcggattt
     3001 ccaagacagg cagtccggta gatatcgttc cctatgtttc cgaccagttt gactatctct
     3061 cggctgatgc agaagataaa tatgttattg ctcaggcgaa tgcgccgttg actgagaata
     3121 agcaatttgt gcgtgagcgc gtctcgtgtc ggtatcactc tggcttcatc ttctcaacgc
     3181 cagatgcagt agattatatg gatgtagcac ctcatcaggt tgttggtatc agtgcggcgc
     3241 tcattccatt ccttgagcat gatgatgcga accgtgcatt gatgggctca aacatgcagg
     3301 ctcaggccgt tccgcttgtt cgcccggaaa ttcctctggt ctcaaccggt atggagtatc
     3361 atgcagcgct tgattccggt caggtggtcg tggcagaagc cgatggtgag atcacttctg
     3421 ttaccggcaa ttcgatcaca gtcaaggaac ggggcggaat ccgtaattat gttctccgca
     3481 aatatcagcg ttctaatcag agcacttgca ttgatcagcg tcctgcagtt gtaaagggac
     3541 aaaggatcaa gaagggcgat attatcgctg actcttcgtc caccgaaagt ggtcagttgg
     3601 cacttggcca aaacgtagtg gttgccttcc tctcgtggga aggtggcaac ttcgaagatg
     3661 cgatcctgat ctcagaacga ctggttcagg aagatcgatt tacttctgtt catattgaaa
     3721 agtatgaagt ggaagcacgc gatacgaagc tgggtcctga agagatcaca cgcgacattc
     3781 ccaatgtcgg tgaagatgcc atcaaggatc ttgatgagaa cggcatcatc cgcattggcg
     3841 ctgaggtggg tccgaacgat attctggtgg gcaagatcac accaaaaggt gaaaaggaac
     3901 tgacaccgga agaacgtctc ttgcgggcga tcttcggcga aaagtcgcgt gatgtaaaag
     3961 atacctctct gcgaatgccc catggcgaac gcggtaaggt tgtagatgta aaagtcttca
     4021 cccgtgaaga gaactctgat ctttctgctg gtgtggatat gatggtgcgc gtttccgtcg
     4081 ctcagcgccg aaaattgacg gcgggcgaca agatggcagg tcgccatgga aacaagggcg
     4141 tggtctcgaa ggttgtgcct gtggaagaca tgccattcct tgaagatggt acaccagttg
     4201 atatcatttt gaatccgttg ggcgttccgg gtcgtatgaa tatcggtcag gtcctggagg
     4261 tccatctcgg atgggccgcg aagcgacttg gctatcgcgc cgttactccc gtttttgacg
     4321 gtgcatcaga agaggaaatt gaagccgaac tcgcgcgcgc ctggattatt gatcaagcct
     4381 ggaaagagac cgcggcgaca tcctgggaat ggatcaagca gcaggaatat gatccgaact
     4441 ccattcagga tgatgatgaa gttcgtcggc tctatctgga agaatggctg ggcgaacggg
     4501 attatgatcc gtatgatctg aacgatgtgg attatgctcg tcacgcgacc gcttctgaat
     4561 ggctccgcga acgcggctat gatccagata cgattttcca ccctgacgac gctgatccgc
     4621 gttcccgtga atcatacaac gctgcagcaa tcagcgcgtg tcttcgattg tggattgaaa
     4681 gccatggaca tggtggacga attgcggaag cgcgcgtcgc cgaggtagca caggagctta
     4741 cgagttcaac gggtcatcct ctgccaattc ttggcaagca gactctgcga gatggtaagt
     4801 ctggcattcc ctatgaccaa cctgttacgg tgggtgtgat gaccattttg aagttgcacc
     4861 acctcgtgga agataaagta catgcccgct ccaccggtcc atacagcttg gttacacaac
     4921 agccactcgg tggtaaggca cagtttggtg gtcaacgatt tggtgaaatg gaagtatggg
     4981 ctttggaggc gtatggcgcc gcttatactt tgcaggaaat gttgaccgtc aaatcagatg
     5041 atgtgcaggg acgtgtcaat acatatgagg cgattgttaa aggtgagccg attgaagagc
     5101 caagtatccc cgcctcgttc cgcgttctgg tcaaagaatt gcagtcgttg gggttggccg
     5161 ttgaagccat caacgaaggt ggcgatgtcg ttaagtttgg taaggatgaa gagaagcagc
     5221 atccgccgaa gctcaataca ggattgttgg gcattggtga taatctgatg accaagcagt
     5281 aaagtaatct tgacgataat tcaagtgagt gataaaatct catttcgttg ccaatagaaa
     5341 aag
//
LOCUS       DASTJP010000367         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1012256, whole genome shotgun sequence.
ACCESSION   DASTJP010000367 DASTJP010000000
VERSION     DASTJP010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012256"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            680..>2955
                     /locus_tag="VFQ23_10860"
     CDS             680..>2955
                     /locus_tag="VFQ23_10860"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9907136.1"
                     /translation="MAVPMLATKLYIPPPRPGVVSRPRLIERLDNGLAKGSRLMLISA
                     SAGFGKTTLVSEWVANCEQPIAWLSLDEGDSDPVRFISYLITALQTIRAGLGEGLLAA
                     LQSHQPPSTEAVLTALLNEIVTVSNDFLFVLDDYHVIDSRSVDETLSFLIEHQPPQMH
                     LVIATREDPTLPLARLRARGQLTELRATDLRFTPAETAEFLNRLMGLNLSDGDIVALE
                     THTEGWIAGLQLAALSMQGREDIASFIQAFTGSHRFVLDYLAEEVLQRQPEHIRSFLL
                     QTSVLDRFCVSLCDAVTTREDAKEILDTLERRNLFLIPLDDQRQWYRYHHLFAEVLQT
                     HLLETQPEQVSILHQQASAWYEQNGLQSDAIRHALVAKDFERAADLIELAGSATEEGS
                     AQSATWLGWVKVLPEELIRARPVLNGWYAYALLGKGDLEAAESRFDDAERWLESADTI
                     ETRPATLAPHAHRPGGSVPGSAGESQSSCGESNRVMVVIDQEQFKSLPATIAIGRAYI
                     AQALGNIPDTVRYASRVLELTEADPFRHSQASMMLGLTHWASGDLEAADGVFADYTRK
                     LRTSGNIPDTISTSVVLADIRLALGRLHEAINTLEQLLQFVMDRGEPIPLDAADLHRE
                     LSKLHLEQGDLEAAAQHLQRSKELGEKAQLPVSRYRLCIDQAWLKTAQGDLEGALALL
                     DEAQRLYIRTPLPDFCPISAMKARIWVAQGGLTKPQGWVREQGLSVDDVPSYLREFEH
                     VTLARVLIARHKSDRETGS"
BASE COUNT          675 a          775 c          785 g          720 t
ORIGIN      
        1 tttccaaagt tggttttctc tcgggctcga taaccagata ccgcagtgtg cctggggcga
       61 gtccgcattg atgtgcaact gctgtcaacg tcaaccttcg gttgtcggct agctgtatta
      121 gccaggtggc aaagggtgaa aggtttttat tttttagcga catgtgtact ccttgaaaat
      181 gcaaacaaaa aacgcctctc ttttcaggga ggcgtttttg gatttagtct tcgggcaaaa
      241 ccgagctttt accttccagg tggattttca gcagcagcag tgcttcctgc accgatgtct
      301 gcggagggat tatcagaaga ggttcgcggc tgtcaggtaa tctcccgtcg ggaccgccaa
      361 atgaacaaca aaaaatcgac ccttttgggg tcgatttttt gttgttttgc ctcaaaaccc
      421 ctcaaaataa taagcccctt agtagttacg cgtcgcgatc tccaagaatc actgtgtttt
      481 atcgaacccg cgatccaaac gcggtcagca atatcctaat ttcagtcttc aaatctgttc
      541 tgtttattaa cattcacgcg acaagctcct tggtttgatg tgccctataa attacgataa
      601 aggcgatttg ttttgaaagc ctaacctcct aatacattgt ccacaccaga tttgtttgat
      661 ttcgtcttat aatgaatcta tggccgtacc aatgctggct acaaaactgt acataccacc
      721 gccccggcca ggtgttgtct ctcgcccacg gctgatcgag cggttggaca atggcttggc
      781 gaaggggagc aggctgatgc tcatttccgc ttccgctggc tttggcaaaa ccacgctggt
      841 aagtgaatgg gttgccaact gcgagcaacc gatagcgtgg ctgtctctgg atgaagggga
      901 tagtgatccg gtacgcttca tctcgtatct aataacagca ttacagacca taagggcggg
      961 acttggcgag gggctcttgg ctgcactcca gtctcatcaa ccgccctcca ccgaagcggt
     1021 tttgacagca ctgctcaatg aaatcgtcac cgtctcgaat gattttctct ttgtcctgga
     1081 tgactaccat gtgatcgatt ccaggtcagt tgacgaaacc ctttcctttc tgatcgaaca
     1141 ccagccaccg cagatgcacc tggtcatcgc cacgcgcgaa gacccaaccc tgccactggc
     1201 tagactacgt gcccgcggac aattgaccga actgcgcgcc actgacctgc gttttacccc
     1261 tgctgagaca gccgagtttc tcaaccggtt gatgggacta aatctctcag acggggatat
     1321 tgtcgcattg gaaacccaca ctgaaggctg gatcgctggt ttgcagttgg ctgcactctc
     1381 catgcaaggc cgagaagaca tcgccagttt tatccaggct ttcaccggta gtcaccgctt
     1441 cgtgctggac tatttagccg aagaagtgct gcaacgccag cctgaacata tccgcagttt
     1501 cttattacag acttcggttc ttgatagatt ctgcgtctct ctgtgcgatg cagtcactac
     1561 gcgcgaagat gcaaaagaaa tactggatac tttggaacgc cgcaaccttt tcctcattcc
     1621 gttggatgac caacgccaat ggtatcgcta tcaccatctc ttcgctgagg ttctgcagac
     1681 ccacttgttg gaaacccagc ctgagcaggt atcgatcctg caccagcagg caagtgcgtg
     1741 gtatgagcag aacggtctgc aatccgatgc catccgccac gctctggttg cgaaagattt
     1801 cgagcgcgcc gcggacctga ttgagctggc aggatccgca acagaggaag gaagtgctca
     1861 gtccgccact tggcttggct gggtgaaggt gctgccagaa gagttgatcc gcgcccgtcc
     1921 agtgctcaat ggttggtacg cctatgcatt attggggaaa ggtgacctgg aagccgccga
     1981 gtctcgattt gatgatgccg aacgatggct ggaatcggcg gatactattg agacacgtcc
     2041 tgcgacactt gcaccgcacg cacaccgccc cggtggttcg gtgccgggga gtgcaggtga
     2101 gtcccagagt tcttgtgggg agagtaaccg agtgatggtt gtgatagacc aggaacaatt
     2161 taagtctctg ccggcgacaa ttgccattgg ccgcgcctat attgctcagg cactcggcaa
     2221 tattccggac acggtgcggt atgccagccg ggtacttgag ctcaccgaag cggatccttt
     2281 cagacacagt caggcatcca tgatgctggg gttgacccac tgggcaagtg gggacctgga
     2341 agctgcagac ggggtcttcg ccgattacac caggaaactg cggacctccg gaaacatccc
     2401 agatacaatc agcacgtccg ttgttctggc tgatatcagg ctggcattgg ggcgtcttca
     2461 cgaggcgatc aatacgctcg aacagttatt acaatttgta atggatcgtg gtgaaccaat
     2521 tcctctcgat gctgctgact tgcacaggga attaagtaag ctgcatcttg aacagggtga
     2581 tctggaggct gccgcgcaac acttgcagag aagcaaggaa ctgggcgaaa aagcgcagtt
     2641 acctgtttcg cgataccgtt tgtgtattga ccaggcctgg ctcaagacag cacagggcga
     2701 cctggaaggg gctctcgcac ttttggatga agcgcaacgc ctgtatatca gaacaccctt
     2761 gcctgacttc tgtcccatat ctgctatgaa agcccggata tgggtcgcgc aaggcggatt
     2821 gaccaaacct caggggtggg tacgcgagca gggcctttcc gttgacgatg tgcccagtta
     2881 cctgcgtgag ttcgagcacg tgacgctggc tcgagtgctc attgcccggc ataaaagcga
     2941 ccgggagact ggctc
//
LOCUS       DASTJP010000368        12808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_553810, whole genome shotgun sequence.
ACCESSION   DASTJP010000368 DASTJP010000000
VERSION     DASTJP010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12808
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553810"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VFQ23_10865"
     CDS             complement(<1..1190)
                     /locus_tag="VFQ23_10865"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET9907137.1"
                     /translation="MLTIDIETARRFILGKQGLWPGRRWRGVRGTEKAMLAMEYLQLD
                     PLQIIARSQDIQLQSRVLDYSPDMWEIPTYKQRKFFDWGGWLAARPMDELPHWRTVMH
                     RERDGNYGDSRIRKMAHEHAAAIIEMRDILQERGIVSNRDFEMATRTRTESYRGRKDS
                     ALALYYLWRTGEVMTHHRDRFERVYALTDQVAPADLIYESSEEETDRFLIEKEISFGG
                     LWRISRTGDSWGRGEPDRAAKKLLGGMLADGKIIEIKIEGWKAVQYALGKYAKMLRDL
                     SAGRIPKAWTPLETTTTEEVLFLAPLDHVSARGRAKALFGFDYVWEVYKPAHLRKFGY
                     YTLPILWGDRLVARFDSKLDRTTNTFIILGLWLENKALGKDEAFAEALARGFARFVKF
                     LGAN"
     gene            complement(1228..1953)
                     /locus_tag="VFQ23_10870"
     CDS             complement(1228..1953)
                     /locus_tag="VFQ23_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907138.1"
                     /translation="MSDHYLLGEFWEAFYIQGYQVGVLNRTTASAADFPNVLMSKLQV
                     VLIVAGEITKYEHVYYFYNRDGFPSHSYLFDAGQGAPVHVRFVDNQMICQVDDDIFTE
                     KVPGDARPSYGYYPLVVTIPFTAGYNLPFTRVDDASCSIEGPAQFVSHGWETVNLQGE
                     RLSLWRVDEYARDRLTNQYWLDEARRIRQSKWLGAVSYWVPTKEKALSGFSPKWSTAV
                     TKTLENSTKIDWSSEVISWLTDK"
     gene            complement(2006..2509)
                     /locus_tag="VFQ23_10875"
     CDS             complement(2006..2509)
                     /locus_tag="VFQ23_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET9907139.1"
                     /translation="MKKTELLDWLQEEYQKFEAFLDQIGETRMEQRGVAGDWSMKDIL
                     AHLTDWNRWLVIRLEAALRSEPNPPPPWPAQAQDDEDINAWIYESNRSRSLHEIRDEM
                     HQVHQQLLAFIEGLPDDVRIERDWRVVWLGDQRFAAGEFFDHFHDDHEPDIRAWLAQQ
                     DDSVSNS"
     gene            complement(2529..3023)
                     /locus_tag="VFQ23_10880"
     CDS             complement(2529..3023)
                     /locus_tag="VFQ23_10880"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9907140.1"
                     /translation="MNADAFRHFYNYHFAENRKIWERYITPLTHEKFTQKVDYSHGSV
                     RDQIVHLLDVEDMWFSELRGANPLEPIPQSDTDDRDFIRTHWDRVEQQIREYLSALQD
                     EMLFTKPIREPEEDQVLFTWQVLLHVVNHATDHRAQILRAVNDLGVETSYQDYIFYVY
                     ENLI"
     gene            3207..4712
                     /locus_tag="VFQ23_10885"
     CDS             3207..4712
                     /locus_tag="VFQ23_10885"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET9907141.1"
                     /translation="MRALLAFLLIVVTALACNRLNPTPTLIPTIIPSATQAVPATEQV
                     ETVEPLDPDAGSSGLGDSLFPGFGNGGYDVTHYTLDITVNDVSTSDLTARTTIEAKAT
                     QTLSSFNLDFVGFEISDISIDDIAAEFERDGQELTITPSTPLEENQSFTVTIEYSGSP
                     DRFVSKSIPFPTGWVRFEGGSYVLSEPDGAASFFPTNDHPLDKASYTFRVTVPEPFEV
                     AANGVLIETVDNGDTNTFLFEARDPMASYLTTINIDEFDIETMESETGTPIRNYYATG
                     LPEGMRKPFARQGEMIDYFGELFGPYPFEIYGSLVLDAQFGAALETQTLSVYGRDMVS
                     ENYVEGTELVVAHELAHQWFGDSVSVADWRDIWLNEGFATYAEGLWVEHRDGREALDA
                     WIKSRYLTLVGMREGVTPPGKPTARSLFNGGVYYWGGITLHALRLEVGDDVFFEILRT
                     YQERYKGSNATTDGFIAIAEEVSGKDMHVFFENWLYSEKIQPIPELGLTAD"
     gene            4794..5333
                     /locus_tag="VFQ23_10890"
     CDS             4794..5333
                     /locus_tag="VFQ23_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907142.1"
                     /translation="MNSKQRIEVDIRPWAEGDLALEERLMGDPAMTEHLGGPESPEKI
                     RERHARYLRLSDSGKGRMFAIVVGPQRVAAGSIGYWEREERGQQVWETGWSVLPEFQG
                     LGVATQATILVLEHARAEGKHRYVHAYPSVDNAPSNALCRKAGFTLQGDEEFEYPPGH
                     FMRCNNWRFDLFPDSSDDQ"
     gene            5323..6405
                     /locus_tag="VFQ23_10895"
     CDS             5323..6405
                     /locus_tag="VFQ23_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907143.1"
                     /translation="MTNKVITSIDQVSVEWLTSALMKSGALAKGEVQSFKFGQGQGNW
                     STSANLFVKYTEDATGSLPERLFLKMVNTDLDDEYFGDSEVTFYTRDYVDVPEAPLLR
                     CYDAAYSKDLHRYHILLDDVSETHIEAAEKEPTLEYGLALADGLAILHARWWGEQKLA
                     EAGAPIHAEGHIQRFVDIAEPGVEHIVKGFSSELKPNWPEAMRELFEMHPKAIIQRAR
                     DLNGFTLIHGDVGSANILVPRHSDRPIYIIDRQPFDWSLTTWLGVYDLAYAIVLDWET
                     ETRRKYEKPILEQYHNQLMRNGVTDYPWEQLHNDYRLCVAMCVYIATEYCRGGVIERL
                     IPAWLPMLQRALTACDDLDCRTLWID"
     gene            6438..6848
                     /locus_tag="VFQ23_10900"
     CDS             6438..6848
                     /locus_tag="VFQ23_10900"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HET9907144.1"
                     /translation="MENLYLVLCYLTISVALIIFGLIHWYRTKRFIETAQRTTGTVID
                     LVSISPKKTTYSPKVRFKAMDGSEILFTEPLATNPPGYELNEQVTVLYDPHDYQRARV
                     FKSKWRIYYFALLFGGLGIIFFIVYMIVLFTIGN"
     gene            6897..7442
                     /locus_tag="VFQ23_10905"
     CDS             6897..7442
                     /locus_tag="VFQ23_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907145.1"
                     /translation="MTLSTTLTWDRTEDLNSAVRDSIIRVCVEAHQEEDFKNLFSYIS
                     SGGLHFLAYHGDQLVSHAAVTTRWLQPEDRPLLKTAYVDAVATLPSHQGRGYGSEVMR
                     QLEREIDREYEIACLETERESFYERLGWKVWRGPLAGRSENGLVPTPHQTGIMILRLS
                     RTPALDLDKALSIECQPDRIW"
     gene            7473..8216
                     /gene="map"
                     /locus_tag="VFQ23_10910"
     CDS             7473..8216
                     /gene="map"
                     /locus_tag="VFQ23_10910"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9907146.1"
                     /translation="MTADSTADIQYLKAIGRICAETLRKMMGVVRVGMTTRELDEIGR
                     AFLEAEGARSAPQAMYKFPGATCISVSPVIAHGIPNERVLREGELIHIDVSAELDGYY
                     ADTGASMVVSKSDRNLERLIEATKAALTKALRAAKAGSPLNGISRSVQTEARKRGYNV
                     IYDLTGHGIGRKLHEEPREILNYYDPKDRRILNEGLVLAIEPFLTTGVGRVVEERDGW
                     SLRTSDHAIAAQFEHTVIVTKNEPIILTA"
     gene            8213..8896
                     /locus_tag="VFQ23_10915"
     CDS             8213..8896
                     /locus_tag="VFQ23_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HET9907147.1"
                     /translation="MINLHLLSTPGDRDIRWVLDACRPYLEGKSEATVAFLPQAWLNV
                     NHWLDYTARSFEKLARVELIDTEQMDLPTMEAIIRRAHVVYISGGNTFLLNHRLHISG
                     LMPYLRKKVQAGLPVIGFSAGMILCGPNILTAKDLNSIGTTHFSSLNASPFNFFAHYV
                     SNTYGESEHDGWLADYHVFHDNPVILLSDGAYVKVDGKKTTLARGDAWILRRGEEKEK
                     LEEGKLIVS"
     gene            9076..9579
                     /locus_tag="VFQ23_10920"
     CDS             9076..9579
                     /locus_tag="VFQ23_10920"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET9907148.1"
                     /translation="MEDEQLVPKNKAELMSAITQEWNLLMDVVTKLQEKDKLTTLDEG
                     GWSPKDNLAHLAEWMNALMGYHMDRRPSHEVFGVPAEVTRDWNMEVINPVLFERNKNR
                     STQDVLDELKRVYEELVAKLNATPFEDLLTPRHADDPERRPVLMWVLGDTTEHFAEHR
                     ETIEKLL"
     gene            complement(9616..9999)
                     /locus_tag="VFQ23_10925"
     CDS             complement(9616..9999)
                     /locus_tag="VFQ23_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9907149.1"
                     /translation="MPTYDFICNNCTQRFDIFLTFAEYGKKTVTCAHCGSKNVRRRMT
                     KVRIAKSEESRMDSMADDFSGFEGLENDPQAMGRMMRKMGKEMGEDMPPEFDEVVDRL
                     EKGQSPEDIESSMPDLGNDMGGDDE"
     gene            10077..10697
                     /locus_tag="VFQ23_10930"
     CDS             10077..10697
                     /locus_tag="VFQ23_10930"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9907150.1"
                     /translation="MAKNYLEGLLGENEEIILETHQHWFVLFGKIFLELLLIAILIGG
                     SLALSVMYPVAIYAVGLVLVPLVGMFNDILVWRNKAYIVTNRRVMQISGVFNKDVVDS
                     SLEKVNDVKMSQSFFGRMFGYGDIEILTASELGVNLFHEINDPIGFKTAMLNAKERMG
                     FDEAGLELRHMSDIPTLIARLDDLRKRGVLSETEFQQKKAELLAKI"
     gene            10872..12671
                     /gene="lepA"
                     /locus_tag="VFQ23_10935"
     CDS             10872..12671
                     /gene="lepA"
                     /locus_tag="VFQ23_10935"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET9907151.1"
                     /translation="MTDHIRNFCIIAHVDHGKSTLADRLLQLTGTISERDMSEQMLDT
                     MDLEREKGVTIKASAVRMYYTAKDNQKYEINLIDTPGHVDFGYEVSRALKACEGAVLV
                     VDATQGIEAQTLANLYQALDADLAIIPVINKIDLPSAHPDEVAEDVGALLGIEPESVI
                     KISAKEGVNVDQILEAIVTRVPPPKDADDVPLRALIFDSHYDAYKGVIAYVRVVEGSF
                     KSNDILRMFATKVDMRPVEIGIFAPGMKPVESLGSGEVGYIATGFKTVHECRVGDTIT
                     RASAPASEPLPGYRTPKPMVFAGIYPVEADDYSDLRESLDKLQLNDASLTFQPETSQA
                     LGFGFRAGFLGLFHMEIIQERIEREYLLDVLFTAPSVEYQAVMIDGETIPVDSPAELP
                     DESKIVEIREPWMNIEIITPTDYYGPIMELVTKRRGIFKQQEYPAPHRVQLDYEIPLS
                     EIIVDFFDDLKSRTKGYASLDYQFLEYRPDELQKLEILVNGEPVDALAAIVHKKDAFY
                     KGQRLITKLKELIPRQLFDVAIQAAAGGRVISRANVKATRKDVLAKCYGGDITRKKKL
                     LEKQKKGKKRLKMVGNVEIPQDAFMAVLKLEEE"
BASE COUNT         3176 a         3205 c         3291 g         3136 t
ORIGIN      
        1 ttgttggcgc cgagaaattt aacgaagcga gcgaaaccac gcgccaatgc ctctgcaaac
       61 gcctcgtctt ttccgagagc cttattctcc agccataagc ccagaatgat aaaggtgttc
      121 gtcgtccggt cgagtttgct atcgaagcgc gccacaagcc tgtcacccca cagaatcggc
      181 agcgtgtaat agccgaactt gcgaaggtgc gccggcttgt atacctccca aacgtagtca
      241 aagccaaaca aagcctttgc acgcccgcgt gcgctcacat ggtcaagcgg cgcgaggaag
      301 agtacctcct ccgtcgtggt cgtttctaat ggcgtccatg ctttcgggat gcgccctgca
      361 cttaaatcac gcagcatttt ggcgtacttg cccagcgcgt actgcacggc tttccaacct
      421 tcgatcttta tctcgatgat cttgccatca gctaacatcc ctccaagcaa cttctttgcc
      481 gcgcggtctg gttcgccgcg tccccacgaa tcacctgtac ggctgattcg ccacaagccg
      541 ccaaagctga tttccttttc gatcaggaaa cgatcggtct cctcctcgct gctctcgtaa
      601 atcaagtccg cgggcgcgac ctgatctgtg agagcataca cgcgctcgaa gcggtcgcgg
      661 tgatgcgtca tcacttcgcc agtgcgccac aaataataca aagccagcgc gctgtccttt
      721 cgaccgcgat aactttcggt gcgtgtgcgc gtcgccattt cgaagtcgcg gttacttacg
      781 atgccgcgtt cctgcaaaat gtcgcgcatc tcgatgatgg ctgccgcgtg ttcgtgtgcc
      841 attttgcgaa ttcgtgagtc gccatagttg ccatcgcgct cgcggtgcat aaccgtacgc
      901 caatgcggaa gttcgtccat cggccgggcg gccagccagc caccccagtc gaagaacttg
      961 cgctgcttgt acgttgggat ttcccacatg tctggactgt aatcgagcac gcgactttgc
     1021 aattggatgt cctgactgcg ggcaatgatt tgcaatggat cgagttgaag gtattccatc
     1081 gccagcatgg ctttctcagt gcctcttacg ccgcgccaac gacgccccgg ccacaatccc
     1141 tgtttgccaa ggataaaacg ccgagccgtt tcgatgtcaa tggttaacat gatgtttaaa
     1201 tgactcctga ataagggggc aaaagaacta cttgtcagtc agccaggaga tgacttctga
     1261 tgaccaatcg attttcgtag agttctccag cgtctttgtc accgcggtgc tccacttcgg
     1321 tgaaaaaccc gataaggctt tttctttcgt cgggacccaa taggaaactg cgcccagcca
     1381 ttttgattgt cgaatgcgac gcgcctcatc gagccaatac tgatttgtca gccgatccct
     1441 ggcatactca tcgactcgcc aaagtgaaag gcgttctccc tgcaagttga ccgtttccca
     1501 gccgtgcgac acaaattgcg caggaccttc gatactacac gacgcatcgt ccacccgagt
     1561 gaagggcaaa ttataaccag cggtaaacgg aattgtaacc accagcgggt agtatccgta
     1621 acttgggcga gcatctcctg ggaccttctc tgtgaagatg tcatcatcaa cctgacatat
     1681 catttgattg tctacaaagc gcacatgaac cggcgctccc tgcccggcat caaatagata
     1741 ggagtgagag ggaaaaccat cgcggttgta aaaataataa acatgttcat acttcgtgat
     1801 ttcacctgca acaatcaaca caacctgcag tttgctcatc agaacgttag gaaagtctgc
     1861 ggcagaggcg gtggtacggt ttagaacccc cacttgatag ccctggatgt aaaaggcttc
     1921 ccagaattcg ccgaggaggt aatggtcgct catagaaaca gatcctgatc gttatggtgg
     1981 cgatgctctt aagttacgaa acaagctacg agttggacac cgaatcgtcc tgctgcgcga
     2041 gccaggcgcg tatatctggc tcatggtcat catggaaatg atcaaagaat tcaccagcgg
     2101 cgaagcgctg atcgcctagc cacaccacac gccagtctcg ctctatgcgc acatcgtccg
     2161 gtaagccttc aatgaaggca aggagttgct gatgtacctg atgcatttca tcccgtatct
     2221 catgtagcga gcgcgaacga ttggactcat agatccaggc gttgatatcc tcatcgtctt
     2281 gtgcttgagc cggccagggc ggtggcgggt tgggttcact tcgcagcgca gcctctaagc
     2341 ggatgacaag ccagcgattc caatcggtaa gatgcgccag gatgtccttc attgaccagt
     2401 cgccagccac gccgcgttgt tccatgcgag tttctccgat ctggtcaaga aatgcttcaa
     2461 atttctgata ctcttcctgc agccaatcta aaagttcggt tttcttcatc catcactcct
     2521 ttacaagctc agattaagtt ctcgtaaacg tagaagatat aatcctgata ggaggtctcg
     2581 actccaaggt cattcactgc ccggaggatc tgggctcgat ggtcagtggc gtgattcacc
     2641 acatgcagaa gcacttgcca tgtaaagagg acctggtctt cctcgggttc cctgatcggt
     2701 ttggtgaaca acatttcgtc ctgtaacgcg gacaaatatt cacggatttg ttgctcgacg
     2761 cgatcccagt gggtgcgaat gaaatcacga tcgtcagtgt cactttgggg aatcggttcc
     2821 aacggattcg cacctcgcag ctcgctgaac cacatatctt ccacgtcgag caggtgaacg
     2881 atttggtctc gaacagaccc atgagagtaa tctacttttt gcgtgaactt ttcatgagtt
     2941 agcggagtga tataacgctc ccagatcttg cggttttcag cgaagtgata attgtagaaa
     3001 tggcgaaagg catctgcgtt catcttttag ttactcctct atcggcgaat aatagaatta
     3061 taatagtacg gatgttctaa taatgtcaaa gtatattcta ttcgcctgct gtgtcaatac
     3121 agccaaaggt tttccggtat gaagacaatt cctgaaaaaa taatccctgg attcctgtgc
     3181 taaactcaag gtctggaagg agattcatgc gcgcgcttct tgcttttctc ttgatcgtcg
     3241 tcacagccct ggcttgcaat cgactcaatc caaccccgac tttgatacca acgatcatac
     3301 cgtcggcaac ccaggcggtt cctgccaccg agcaggtcga aacggttgag ccgctagacc
     3361 cggacgcggg ctcctccggg ctgggcgatt cgctctttcc gggatttggc aacggtggct
     3421 atgacgtgac tcactacacc ctggatatca cagtaaatga tgtgtccacg agtgatctca
     3481 cggcaaggac aacgattgaa gccaaagcga ctcagactct cagcagtttc aacctggact
     3541 ttgtcgggtt tgaaatttca gatatcagca tcgatgatat tgccgcggaa tttgaacgtg
     3601 atgggcagga attgacgatc acaccttcca cgcctctgga agagaaccaa tcgtttactg
     3661 taacgatcga atattcaggc tcgccagacc gattcgtttc caagtccatt ccatttccaa
     3721 caggctgggt aaggttcgag ggtggtagtt atgttctcag tgaaccggat ggtgcggcta
     3781 gttttttccc cacaaacgat catccactgg ataaagcctc gtataccttc cgcgtgacgg
     3841 tacctgaacc gttcgaagtg gctgccaatg gagtactgat agaaaccgtt gataacggcg
     3901 atacgaacac gttcctcttc gaagcgcgcg accctatggc aagttatctg accacgatca
     3961 acatcgatga attcgacatt gaaaccatgg aatcagaaac tggaacgccc atccggaatt
     4021 actacgcgac ggggttacca gaaggcatgc gcaagccttt tgcgcgccag ggtgaaatga
     4081 tcgattattt cggtgagttg tttggaccct atccctttga gatctacggt tcactggtgt
     4141 tggatgctca atttggtgca gccctcgaga ctcaaacgct gtccgtctac ggacgcgaca
     4201 tggtgtccga aaactacgtg gaaggcacag agctggtcgt cgcccacgaa cttgcgcatc
     4261 aatggtttgg ggacagtgtc agtgttgcgg attggagaga catctggctc aacgaagggt
     4321 tcgcgaccta tgctgaaggt ctatgggtgg aacaccgcga tggtcgcgag gcactggatg
     4381 cttggatcaa atcacgctat ctgacgctgg tcggcatgcg tgaaggtgtc actccgcccg
     4441 gcaagcccac agcgcgtagt cttttcaatg gtggtgtgta ctattggggt gggatcactc
     4501 ttcacgcctt aaggcttgaa gtgggtgatg acgtcttttt tgagatcctg cgaacgtatc
     4561 aggaacgata caaaggcagc aatgcaacca ctgatggttt tatcgccatt gcagaggaag
     4621 tcagcggaaa agatatgcac gtgttctttg agaactggct gtacagcgaa aagatacagc
     4681 ctatcccaga gctgggattg accgccgatt aagagcgttc aatatctgtt ccgagcgaac
     4741 agagcgagca atctagtcgt tgtttgtgat ataggtctaa tgaggagaat aatttgaaca
     4801 gtaaacaacg aatcgaagtg gacatccgac cctgggcaga aggtgatctt gcactcgaag
     4861 agcgactaat gggtgacccg gcgatgactg agcatctggg tgggccagaa tctcctgaga
     4921 agattcgcga acgccatgcg cgttatctgc gcctttcaga ctctggaaaa ggtcggatgt
     4981 ttgccatcgt agtcggaccg cagagagtcg ctgccgggtc aattggctac tgggagcgag
     5041 aagagcgggg ccagcaggtt tgggaaacag gctggagcgt gcttccggag ttccaggggt
     5101 taggtgttgc gacgcaggct accatcctcg tgctcgaaca cgcccgcgca gagggaaagc
     5161 atcggtacgt acatgcctat ccgtcggtgg ataacgcgcc ctcgaacgcc ctctgccgaa
     5221 aggcaggttt tacactccag ggtgatgaag aattcgaata cccgccgggt catttcatgc
     5281 gttgtaataa ctggcgcttc gatttgttcc cagactcttc agatgaccaa taaagttatc
     5341 accagcatcg accaggtttc cgtcgaatgg cttacgtccg cactgatgaa gagtggtgcg
     5401 ttagcaaagg gtgaggtcca gtcatttaaa ttcggtcaag gccaggggaa ctggtcaaca
     5461 agcgccaacc tcttcgtgaa atacacagag gatgctacag gttcactgcc agagcgatta
     5521 tttctaaaaa tggtcaatac ggacctggac gatgaatact ttggcgattc agaagttacc
     5581 ttttacaccc gcgattatgt ggatgtgcct gaagccccgc tccttcgttg ttatgatgca
     5641 gcatattcga aagacctcca tcgatatcac attcttttgg atgatgtttc agaaacccat
     5701 atcgaagccg cggagaaaga accgactctc gaatatgggc ttgccctcgc ggacggatta
     5761 gcaatccttc atgctcgctg gtggggtgaa cagaaacttg ccgaagcagg cgcgccgatt
     5821 cacgctgaag gtcatattca gcgtttcgtg gatattgccg agcctggggt tgaacatatc
     5881 gtcaaaggat tctcttcgga attgaaacca aactggcccg aggctatgcg cgagttattt
     5941 gaaatgcatc caaaggccat tatccaacgg gccagagacc tgaatggctt caccctcatt
     6001 catggtgatg ttggctctgc caatatcctt gtgcctcggc actcggatcg cccgatctat
     6061 atcattgatc gccagccgtt cgattggagt ttgaccacct ggctgggtgt ttacgacctg
     6121 gcgtatgcca tcgtgctgga ttgggagacc gaaacgcgaa gaaaatatga aaaacccatt
     6181 cttgagcagt atcacaacca gcttatgagg aacggtgtga ctgactaccc ctgggaacaa
     6241 ttacataacg actacagatt atgtgtggca atgtgtgttt atattgcaac ggagtattgt
     6301 cggggtggcg tgattgagcg attgatccct gcctggctgc cgatgctgca gcgagcgctc
     6361 acggcctgtg atgacctgga ttgtagaaca ctttggatag attgagtaca cgttgcgttt
     6421 gatgccttcg caaaataatg gaaaatctct atctcgttct ttgttacctg accatttccg
     6481 tagctcttat tatttttgga ttgatccatt ggtatcgtac aaagcgattc attgaaactg
     6541 cccaacggac caccggcacg gtcattgatt tggtgtcaat ttccccgaag aaaaccacct
     6601 attcgccaaa ggttcgtttc aaagcgatgg atggaagtga gatcctgttt acggaacctc
     6661 ttgccaccaa tccgccgggg tatgagctca acgaacaagt gactgtactt tatgatccac
     6721 acgattacca gcgtgcgcga gtattcaaat caaaatggag gatttactac ttcgcgcttc
     6781 ttttcggagg tcttggaatt atttttttca ttgtttatat gattgtactg tttacaattg
     6841 gcaattaata tcagtgatct tagaaacaaa aaaatcatta gtaggaaatt taaagaatga
     6901 ctctatcaac caccttaacc tgggatcgca cggaagacct gaattcagcg gtacgagatt
     6961 caattattcg agtatgcgtg gaggcacatc aggaggaaga tttcaagaat ctcttttctt
     7021 atatctcctc gggtggcttg cattttcttg cttatcacgg tgatcaactg gtgagtcacg
     7081 cagcagtgac cacacgctgg ctccaaccgg aagatcggcc tcttttgaaa acagcctatg
     7141 tggacgcggt ggcgacactg ccgtctcatc aggggcgcgg ttatggaagt gaagttatgc
     7201 gccagttgga aagggaaatt gatcgtgaat acgagatcgc gtgtttggag accgagcggg
     7261 aatcatttta tgaacgcctc ggctggaaag tctggcgcgg tccactggca gggcggagtg
     7321 agaatggatt ggttcccacc ccacatcaga ccggcatcat gattttgcga ttgtcgcgaa
     7381 cccctgcgct tgatttggat aaggcgctat ccattgaatg ccaaccagat cgcatctggt
     7441 agtgtttgtt cgaactgcat tacaatcaaa ccatgactgc cgactctaca gctgatattc
     7501 aatatctcaa agccattggg cgaatttgcg cggagaccct gcgcaaaatg atgggagtag
     7561 tacgtgtggg aatgaccacg cgcgaactcg acgaaatcgg gcgcgccttt ctcgaagccg
     7621 agggagcacg ttctgcaccc caggctatgt ataaatttcc cggtgccacg tgcatcagcg
     7681 tttctccggt catcgcacat gggattccaa atgagcgtgt attacgcgag ggcgagttga
     7741 tccacatcga cgtttctgcg gaactggatg gttattacgc cgatactggc gcatcaatgg
     7801 tcgtttcaaa aagcgatcgc aacctcgaac gattgattga agcaaccaaa gccgccctca
     7861 caaaggcttt gcgcgcagca aaagctggaa gtccgctaaa cgggatcagc cgctccgtcc
     7921 agacagaagc aaggaagcgc ggctataacg tcatctatga tttgacgggt cacggcattg
     7981 ggcgcaaact gcatgaagag ccgcgcgaaa tcctgaacta ttatgaccct aaagaccgcc
     8041 gcattctgaa cgaaggcttg gtgctggcaa ttgagccgtt ccttaccacg ggcgtcggac
     8101 gcgtggtcga agaacgggat ggctggtccc tgcgcacctc ggatcatgcc attgccgctc
     8161 agttcgagca tacggtaatc gtcacgaaga acgagccgat cattttgacg gcatgatcaa
     8221 cctccatctt ctttccacgc ccggcgaccg agatatacgt tgggttttgg atgcgtgtcg
     8281 cccgtatctc gaagggaaat cagaagctac ggttgcattt ctgccgcaag cctggttgaa
     8341 tgtcaatcac tggctggact ataccgctcg atcttttgaa aagctggcac gtgtcgaatt
     8401 gatcgacacc gaacaaatgg atttgcccac tatggaagct attatcaggc gtgcccatgt
     8461 ggtttacatt tcaggtggaa atacatttct gctcaatcac cgcctgcata tcagcggctt
     8521 gatgccttat ctgcgtaaaa aagttcaggc aggcttgccc gtgattggct tcagcgctgg
     8581 gatgattttg tgcggaccaa atattctcac agcaaaagat ttgaactcca ttgggacgac
     8641 ccatttcagc agcttgaacg cgtcgccctt caatttcttt gcccattacg tttcaaatac
     8701 ttatggagag tctgaacatg atggctggct ggctgattat cacgtctttc atgacaaccc
     8761 ggtgatcctg ctgagtgatg gtgcgtatgt aaaagtggac gggaagaaaa caactctcgc
     8821 gcgcggcgat gcgtggatac tgaggagggg agaagagaaa gaaaagcttg aagaaggaaa
     8881 gttgattgtt tcctaaagta accatcgtca ttgcgaggag cccgacactt gcgctggcac
     8941 gctagtgcag gtgtagggcg acgtgacaat ctcctaatta acgcggagat tgcttcgggc
     9001 gaacaagcat cgtcctcgca acacttgcac cgcatgcaag tgcaggtgtg acaataatgt
     9061 attcggaggt ttattatgga agatgaacaa ttggttccca aaaacaaagc agagttaatg
     9121 tctgccatca cacaggagtg gaatctcttg atggatgtgg taacaaaact gcaggaaaaa
     9181 gataaattga cgacactcga tgaaggtggc tggtctccaa aagataacct tgcccatctc
     9241 gccgaatgga tgaacgccct gatgggctat cacatggacc ggcgtccgtc gcatgaggtg
     9301 tttggcgtcc ccgcagaggt gacgagggat tggaatatgg aagtgatcaa cccggtctta
     9361 ttcgaaagaa ataagaatcg ttcgacgcag gatgtgctgg atgaactgaa acgtgtctat
     9421 gaagaactcg ttgcgaaact gaacgccaca ccgtttgaag acctgctaac gccgcgccat
     9481 gcggatgacc ccgagagacg ccccgttttg atgtgggtgc tgggcgatac gaccgagcat
     9541 tttgcggaac accgcgagac gatagagaaa ttattatgaa ttttgtaggg gcacgacatg
     9601 tcgtgcccct acggtttatt catcatcccc gcccatatca ttccccaaat ccggcatcga
     9661 cgattcgata tcctccgggc tttgaccttt ctcgagtcga tccacaactt catcgaactc
     9721 gggcggcata tcctcgccca tctctttccc catcttgcgc atcattcgac ccatggcttg
     9781 cgggtcattt tcaagccctt caaagccgct gaagtcatct gccatcgagt ccatgcggct
     9841 ctcttcagat ttcgcaatgc ggaccttcgt catgcggcga cgcacatttt tgctgccgca
     9901 gtgcgcacag gtgaccgttt tctttccata ttcggcgaag gtgaggaata tatcaaaacg
     9961 ctgagtgcag ttattgcaga taaaatcata agtaggcata cgtctatttt aacggaatct
    10021 ggctgcatgt tacaataatc atgtcatatg ctctcttaac aatcaggaga aaaataatgg
    10081 caaaaaatta ccttgaaggt ctgctgggtg agaacgagga aatcattctt gaaacacacc
    10141 agcattggtt tgtgctcttt ggcaagatct ttcttgagct cttgttgatc gcgatcctga
    10201 tcggggggag cctggcgctc tctgtgatgt acccggttgc catctacgcg gttggacttg
    10261 ttttggttcc attggtcgga atgttcaatg acattctggt gtggcgcaac aaggcgtata
    10321 tcgtgacgaa ccgccgcgtg atgcagatat caggtgtttt caacaaggat gtggtggact
    10381 cctcgcttga aaaagtgaat gatgtaaaga tgagtcagtc ctttttcggg cgaatgtttg
    10441 gctacgggga tattgagatc cttactgcca gtgaattagg tgtaaatctt ttccatgaga
    10501 ttaatgaccc gatagggttt aaaacagcga tgttgaacgc caaggaacgg atgggctttg
    10561 atgaagcagg tctggaactc cgccacatga gcgacatccc aacgctcatc gcaagattgg
    10621 acgatttgcg caagagaggg gttctctccg aaacggaatt tcaacagaag aaggcagagt
    10681 tattagccaa gatttaatat ctgtaaatgg cacagtaggg gcacagcgaa acctgtccgg
    10741 attttgacaa aaaatcgtct cgctgtgcct ctagtcaatt aaaaagtctc tgcgctctcc
    10801 cctggcaccg cccgccaggg caagtgtgtg gctcagtggc agattatttc ggttatactt
    10861 tccccgctat tatgacagac catatccgca atttttgtat catcgcacat gtagaccatg
    10921 gcaaatcaac gctcgctgac cgcttgcttc aattaacggg cacgatctct gaacgcgaca
    10981 tgagcgagca aatgctggac acgatggacc tcgaacgtga aaaaggcgtc acgatcaaag
    11041 cctccgcagt ccgtatgtat tacaccgcga aggataatca gaaatacgag atcaatctca
    11101 ttgatacgcc cggtcacgtg gactttggtt acgaagtcag ccgcgcgctc aaggcttgtg
    11161 agggcgcagt gcttgtagtg gatgctaccc agggcatcga ggcgcagacg ctggcgaacc
    11221 tctaccaggc actcgatgcc gacctggcaa tcattcccgt catcaacaaa attgatctgc
    11281 cctctgcgca tcccgatgaa gtggcggaag acgtaggcgc tttgttaggg attgaacctg
    11341 agagcgtaat taaaatctct gcgaaggaag gcgtcaacgt tgatcaaatc cttgaagcga
    11401 ttgttacgcg cgtgccgcca cccaaagatg cagacgatgt acctctgcgc gccctcatct
    11461 tcgactcaca ctatgacgcc tataaaggag tcatcgccta tgtacgcgtc gtggaaggct
    11521 cgttcaaatc caacgacatt ctgcgcatgt tcgcaaccaa agtggatatg cgtccggttg
    11581 agattggcat cttcgcgcca ggcatgaagc ctgttgaatc tttgggatca ggcgaagtag
    11641 gttacatcgc cactggattc aaaaccgtgc atgagtgccg cgtgggagat acgatcacgc
    11701 gtgcctcagc tcccgcctct gaacctttgc ccgggtatcg aaccccgaag ccgatggtct
    11761 tcgcggggat ttatccggtc gaagcagatg actacagcga cctgcgcgaa tctcttgata
    11821 agcttcaact caacgatgct tccctgacct ttcagcctga aacttcgcag gcattgggat
    11881 tcggtttccg cgcgggcttt ctgggtttgt tccacatgga gatcatccag gaacgcattg
    11941 agcgcgaata tttgttggat gttctcttta ccgctccctc ggtcgaatat caagccgtca
    12001 tgatcgacgg cgaaacgatt cccgtggatt caccagcaga actcccagat gaatcaaaga
    12061 tcgttgagat ccgcgaaccg tggatgaaca tcgagatcat cacgcctact gactattatg
    12121 gtccgattat ggaactcgtc accaagcggc gcggtatctt caagcaacag gaatatcccg
    12181 ctcctcaccg cgtgcagttg gattatgaaa ttccgctttc tgaaattatt gtggatttct
    12241 ttgacgattt aaagtcgcgc acgaaaggct atgcttcact ggattatcaa ttcctggaat
    12301 atcgcccgga tgaattgcaa aagctggaaa tactggtcaa tggcgaacca gtcgacgccc
    12361 tcgcagcaat cgtccacaaa aaggacgcat tctacaaggg tcagcgactc atcaccaagc
    12421 tcaaggaatt gattccgcgt caactgttcg atgtggccat ccaggccgca gccggcggac
    12481 gagtgatctc gcgcgccaat gtcaaagcca cgcgcaagga cgttctggcg aaatgctatg
    12541 gcggtgacat cacccgtaag aagaaactgc ttgagaagca gaaaaagggc aagaaacgtt
    12601 tgaagatggt cgggaatgtc gaaatcccac aagacgcctt catggctgtt ttgaaattgg
    12661 aagaggaata aaacgtaggg gcggggtttt cccgcccagg gcggcatgac gccgccctac
    12721 gaggaaaccc catgaaagat gcaaaacgcc ttttgcctgg cgcgatcatc agcatattat
    12781 taattgctgc aatcctctat tttgtcga
//
LOCUS       DASTJP010000369         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_118606, whole genome shotgun sequence.
ACCESSION   DASTJP010000369 DASTJP010000000
VERSION     DASTJP010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118606"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFQ23_10940"
     CDS             <1..895
                     /locus_tag="VFQ23_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9907152.1"
                     /translation="FAGGIATVLGYFLSSTVTDRIHLLQAAAEQLAKGDLKTRVPVQG
                     RDEVAMLANAFNRMAEQLQAMDQKQRELENMRRDLIAWVSHDLQTPLTSMRAILEALS
                     DGVVDEPEMVNRYLNTAQRDVRSLSALIDDLFQMAQLDAGGFPLSRAQASLSDLVSDT
                     LESFSELAYRDSITLQGKVEPNVDPVFMDTQAIGRVLNNLISNALRHTSAGGKIHVSA
                     RRMVEGVELTVSDTGEGIRAEDIPHIFESFYRGEKSRNRGTGGSGLGLAITRGILRAH
                     GGDIRVESEPGKGTRFIFHIP"
     gene            1029..1583
                     /locus_tag="VFQ23_10945"
     CDS             1029..1583
                     /locus_tag="VFQ23_10945"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9907153.1"
                     /translation="MKQLLIGLTAFILFGCAPGTTMRQQTDSAMPMIDSLPDLGPAPE
                     LTNEIWLNVDSPLRLADLRGKVVILEMWTFGCINCQNVMPSLKEWHSKYKDQGLVIIG
                     NHFPEFSYEADLANLKDAIARNDIQYAVAQDNDGATWRAYRNHYWPALYLIDKQGHIR
                     YVHIGEGRYKETEENIKTLLEENY"
     gene            1592..>2045
                     /locus_tag="VFQ23_10950"
     CDS             1592..>2045
                     /locus_tag="VFQ23_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9907154.1"
                     /translation="MNKIKSVPVKSSEITSYSQYLSRRDFLKAAGIMTGSALLAACAP
                     SGTESTEALDVPTSNQTDEFGDPVNSFTDITNYNNFYEFSTNKEAVAPLSKNFNPSPW
                     TVEVYGLVNNPKTYGIEDLLSKFTQEERIYRLRCVEAWSIVIPWTGFPL"
BASE COUNT          551 a          498 c          492 g          504 t
ORIGIN      
        1 ctttgcaggc ggaattgcca ccgtgctcgg gtattttctc tccagcaccg tcactgaccg
       61 gattcatcta ttacaagcag cagctgaaca actggcaaaa ggagatttga aaacgcgcgt
      121 ccccgtgcag ggacgtgacg aagttgccat gctggctaat gcattcaatc gaatggcaga
      181 acaacttcaa gctatggatc aaaaacagcg cgagttggaa aacatgcgcc gcgatctcat
      241 tgcatgggtc agccatgacc tgcaaactcc ccttacgtcc atgcgagcca tcctggaagc
      301 gctttccgat ggcgttgtgg acgagcccga aatggtaaac cgttatctca acaccgccca
      361 gcgcgacgtg cgctccctct ccgctctgat cgatgacctg tttcaaatgg cacaactcga
      421 cgcaggtggt tttccgctga gtcgtgcaca ggcatcactc agtgaccttg tttcagatac
      481 tttggaaagt ttctccgagc ttgcgtatcg agacagcatt acactccaag gtaaagtaga
      541 gccaaatgta gatccggttt ttatggacac acaggccatt ggcagggtat taaacaacct
      601 gattagcaat gccttacggc atacatccgc cggtgggaag attcatgtca gtgcgcggcg
      661 aatggtcgaa ggcgttgaac tgactgtcag cgacaccggc gaaggcatcc gcgcggaaga
      721 catcccacat atctttgaga gtttttatcg tggtgagaag tcgcgcaacc gcggcacagg
      781 cgggtcaggc ttgggcttgg cgattacgcg cggcatccta cgtgcccacg ggggtgatat
      841 ccgagttgaa agcgaaccgg gaaaaggcac tcgttttatt tttcatattc cctgatcttg
      901 agaccctaaa gttttacctt ttcactttgt tcccggggtg cttcgtaaaa cctttagggt
      961 ctattaattt aatcgtaatc ctttcgtaag gaagtttact tagaatgaag tcatcaagga
     1021 ggctgacaat gaaacagcta cttatcggat tgactgcctt cattctattt ggatgtgcgc
     1081 caggaaccac tatgagacaa caaacagatt ccgcaatgcc catgatcgat tcactaccgg
     1141 acctcggtcc tgctcccgaa ctaacaaacg agatttggct gaatgttgat tcgccgctgc
     1201 gtctcgcaga tttacgcggc aaagttgtca tcctcgaaat gtggacattt ggctgtatca
     1261 actgtcagaa tgtgatgcct tcattgaagg agtggcactc gaaatataaa gaccagggat
     1321 tggtcatcat cggcaatcac ttccctgaat tttcgtacga agcagatctc gccaatctga
     1381 aggacgcaat tgctcgcaat gatattcaat atgcggttgc gcaggacaat gatggcgcaa
     1441 cctggcgagc ctatcggaat cattattggc cagctttata tctgattgat aagcagggtc
     1501 acatccgtta cgtgcatatc ggcgaaggaa gatataaaga aacagaagaa aatattaaga
     1561 cacttttaga agagaactat tagaggtaat tatgaacaaa atcaaatctg taccggtcaa
     1621 atcatctgaa attacatcct actcgcaata tctctcgcgc cgcgactttc tcaaagccgc
     1681 gggcataatg actggttccg cgctattggc ggcctgtgca ccgagcggaa cagagtcaac
     1741 ggaggcactg gacgtgccga cctcgaatca aacggatgag tttggagacc ccgtcaactc
     1801 atttactgac attacaaact ataataattt ttacgaattc agcacaaata aggaagctgt
     1861 tgcaccgctc tccaagaatt tcaaccccag cccatggacc gtcgaagttt atggattggt
     1921 caacaatcca aaaacctatg gcattgagga tctgttaagt aaattcaccc aggaagaacg
     1981 catctatcgg ttaagatgtg tggaagcctg gagtattgtc atcccatgga ccggttttcc
     2041 gcttg
//
LOCUS       DASTJP010000370         5613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_971105, whole genome shotgun sequence.
ACCESSION   DASTJP010000370 DASTJP010000000
VERSION     DASTJP010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971105"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFQ23_10955"
     CDS             <1..608
                     /locus_tag="VFQ23_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907155.1"
                     /translation="GLDEVVIGEMMCSHKSGIRYIASPRYPIAADTFSNEILKHFMDG
                     LKSHNQFIVVDTSHDFSDMTIQMLTASNTILLVMAPEMASLRTTMSALEIYDRLGISL
                     DKVRIVLNNNSSNPAIKQAQLEKVLKRAVDFVLPFEAGEVNRALNFGRPFILENPDLP
                     ICVNLEKIAYELSDQAYKSLPPAVPTSTWKRVTSQLSNKQG"
     gene            608..2503
                     /locus_tag="VFQ23_10960"
     CDS             608..2503
                     /locus_tag="VFQ23_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907156.1"
                     /translation="MRKKNGQSAYLILFILGIGLPGFVGILGVQAQAEAVELHSASSN
                     LQTTNTATPTPTQVPSTFTSPALIATDFPSSNLATPAGIFSPGLYAFIQAPNGSVANP
                     YVILSAFSSLSGSKDVTIRGFINSAEFICTQSPCIIPVPSNARFVFRAFSDSGVSSNE
                     VIATVRVSQVSDGYLVTIESVTQFATFIDSCSQAWGVQDETNSTWASFVQFPFQLNTR
                     KTLHTLATQLILNGIVDTSNCPAGGLNLSLSWPTACGLERASTEMIAWQNQFDEYIWL
                     ASKNYGIPPKILKTLIEVESQFWPGNSRFYLDEYGLGQLNQLGGDVLLRRDPVLYQQA
                     CAGVFADCLRPYQSLEPSQQALIRGAVVNMADASCPTCQYGIDLEKAKESVSLIAQLL
                     RANCQQVDVILNIAARSFIDEDADAATATAAVATATAAGGGLSRTTYEDLWRFTLVAY
                     HSGLSCLQTAVTTIEKARLPVTWENVSGEIKCRGGEDYADGFMNNLFSFDLYLNQFSE
                     ADIVLAAPTFVPTQTPIPTPTVYISTAQIRVEVYIDRNNNGAPENGEWIDAMSVLLRT
                     STNDQITQRTQNGIAIFDMTGYPPGIDIHLSLPGLYRDETFVLPEQGEVLVRFVFEQP
                     TLPTVLP"
     gene            2516..3772
                     /locus_tag="VFQ23_10965"
     CDS             2516..3772
                     /locus_tag="VFQ23_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907157.1"
                     /translation="MSKKNRKQDRAAKLFDKTEIESIEAHVESKESAEAANMDDSPEL
                     IETEEITETSRSEEPNSETLSNSENGDVSVDEQPEAQEAETSTDLLFDDVRQSLIEDE
                     TVQQNKKSKKWWRRVAKGLQKEKSPEAVSTPVEENSVVPVNQESDVTETKAEEVPSDV
                     DEIDELINMLETESAEVSFTAPVKTEPEVAVEPEIEVDIEELKKQALRPRGEIEEQET
                     ITEVRSVALEDGEEVFVEVESTPQDPLEERLSALENALRPYRRYIYFGFTVFGIVLAL
                     IASAVLFNVYRQSRPAAPTEVSNLPYPTAIRLPGGLNFTLGKGSLQAGQWNPQGPEWL
                     QGTEVCRWIAIPWSTQIEAVIRTLNPEDPIELTMSNNDHLVYKVYSIQQLTNEELQRL
                     PSDSPCLMLILAKAETETRWVLTALP"
     gene            4180..5358
                     /locus_tag="VFQ23_10970"
     CDS             4180..5358
                     /locus_tag="VFQ23_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HET9907158.1"
                     /translation="MFIKKFHRQVHFAGFRAQAIVEFAIALPILMALLVGIFEVGRMV
                     FIYSAVTNASREASRYGSAVGLDDTGSYAKYQYCDGIRAMARRSAYFLNLADSDILIE
                     YDNGPSTSVFDTCTSNVDTAVHVNSGAEPDRVIVTISTDYSPMITLIPISSRTITSSS
                     ARTILGILDLNSGNSVVPTTGPTNTSAPTVPATALPSDTPTVKPTGPSDTPTATEPGG
                     DLITFTPLPSSTMTDTPTITPTFTATFTPTMTFTPTVTFTPTTTSTPVPGCGNILAGT
                     INTANSSNLMTMTITNPHDAVTVSNIHVTWNSTTGASGSKPLTLLSVSLGTTFWTGNS
                     TAGSGLTITPSTTLTIPGNNRISTIIFSFDNKYHNPIGNSITINLSTIGCESNPIQRP
                     "
     gene            5390..>5613
                     /locus_tag="VFQ23_10975"
     CDS             5390..>5613
                     /locus_tag="VFQ23_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET9907159.1"
                     /translation="MKPIHSERGQALIILALAAIGIFGIVGLAIDGSAKFSDRRHAQN
                     AADSAALAGALALVNEDTTWVLDALNRALE"
BASE COUNT         1474 a         1355 c         1353 g         1431 t
ORIGIN      
        1 atggactcga cgaggtggtc atcggagaaa tgatgtgctc ccataagagc ggaattcgtt
       61 atatcgcctc acctcgatac ccaattgcag ccgatacctt ttcaaatgag atcctgaagc
      121 acttcatgga tggactaaaa agccataatc aattcattgt cgtggataca tcccatgatt
      181 tttcggacat gacgattcag atgttgacag cttccaacac aatactatta gtgatggcac
      241 ccgaaatggc ttcgctgcgt acgacgatga gtgccctgga gatttacgac aggcttggga
      301 tatctttgga taaggtcaga atcgtgctga acaataattc ttccaatccg gctatcaaac
      361 aggcacagct tgagaaggtt ctgaagcgcg ctgtggattt tgttctgccc tttgaggcag
      421 gggaagtgaa ccgtgctctg aattttggcc gaccgtttat tttggaaaat cccgatttgc
      481 ccatttgcgt aaatttggag aagatagcct acgaattaag cgaccaggca tacaaatcgc
      541 ttccgcctgc ggttccgaca tcaacgtgga aacgggtaac cagtcaactt tcgaataagc
      601 aggggtaatg cggaaaaaaa acggtcaatc cgcttacctg attctcttca ttcttggaat
      661 tgggcttccg ggcttcgttg ggattttagg agtccaggcg caggcagagg ccgtcgaact
      721 ccactcggca agctcaaatc tccaaactac caatactgcc acgccgacac ccacgcaggt
      781 cccgtccacg ttcacctctc cggcattgat cgcaaccgat ttcccatcca gcaatctggc
      841 cactccggct ggaatttttt ccccaggctt gtacgcattt attcaagcac ccaacggatc
      901 ggtcgccaat ccctatgtga ttctgagtgc tttttcttcc ttgtcaggta gtaaggatgt
      961 aacgatcagg ggctttatca attcggccga atttatttgc acgcaatctc cttgtattat
     1021 tcccgttccg tccaatgcac gatttgtttt cagggctttt tctgattcgg gggtttccag
     1081 caatgaagtg attgctaccg tgagagtctc tcaagtctcg gacggatatc tggtcacaat
     1141 tgaatcagtt actcagtttg ccacttttat cgattcctgc tcacaagcct ggggcgttca
     1201 agatgaaaca aattccacat gggcctcgtt cgttcagttc ccatttcaac ttaacaccag
     1261 gaaaaccctt catacattag ccacacaact catcttgaat gggattgttg atacaagtaa
     1321 ttgccctgct ggtggactta atctaagttt aagttggcca actgcctgcg gattggagcg
     1381 tgcttcaacc gagatgatcg cgtggcagaa tcagtttgac gagtatattt ggttggccag
     1441 taagaactac ggtatacccc caaaaatcct aaaaaccttg attgaggtcg agtcccaatt
     1501 ttggcccgga aattcacgtt tttatctgga tgaatatggt ctcggccagc tcaatcaatt
     1561 gggcggggat gtgctgttgc ggagggatcc ggtactatat cagcaggcct gtgccggggt
     1621 ctttgcggac tgtctaaggc cttatcaaag cctggaaccg tcacagcagg ctttaattcg
     1681 aggggccgtt gtaaacatgg ccgatgccag ttgcccaacc tgccagtatg gaattgatct
     1741 cgaaaaagca aaggaaagtg tttcacttat tgcgcagttg ctccgggcca attgccagca
     1801 ggtggatgtc attctcaata ttgccgcaag gagctttata gatgaagatg cggatgcagc
     1861 taccgcaacc gccgctgtcg cgaccgctac agctgccgga ggcggtcttt ctcgaacgac
     1921 gtatgaagat ctttggcgtt ttacgttggt cgcttaccat agcggactca gctgtttgca
     1981 aacggccgtg acgaccatcg agaaggcaag attgcctgtt acctgggaaa atgtgtcggg
     2041 cgagatcaag tgcaggggag gagaagatta tgccgacgga ttcatgaata atcttttttc
     2101 ctttgatctc tatcttaatc aatttagtga ggctgacatt gtgctggcag caccaacctt
     2161 tgtgcccacg cagacaccga ttcccactcc gaccgtttat atatctactg cgcaaatccg
     2221 agtcgaggtt tacatcgatc gaaataacaa tggtgctccc gaaaacggcg aatggattga
     2281 tgcaatgtcc gttcttcttc ggacatccac aaatgatcaa atcacgcaaa gaacacagaa
     2341 tggcattgca atatttgata tgacaggtta tccgccaggg attgatatcc acctgagctt
     2401 gcctggtctg tatcgcgatg agacctttgt tttaccagaa cagggcgaag tcctggtgag
     2461 gtttgtgttt gaacagccca cattacccac agttctgcca tagtgagtaa ataccatgtc
     2521 aaagaaaaat agaaagcaag atcgtgccgc aaagctattt gataagacgg aaatcgaatc
     2581 cattgaggcg catgtcgaat cgaaggaatc agccgaagcc gcgaacatgg acgatagtcc
     2641 cgaactcata gaaacggaag aaatcacgga aacgtctcga agtgaagaac caaattctga
     2701 gacgttgtca aattcggaaa acggtgacgt ttccgtcgat gagcaacctg aagcgcagga
     2761 agcagaaact tccactgatt tgctttttga tgatgttcga cagtccttga ttgaggacga
     2821 aactgttcag caaaacaaaa agtcaaaaaa atggtggcgc cgggttgcca aaggccttca
     2881 aaaagaaaaa agccctgaag cggtatcgac gcccgtggaa gagaactctg tggtgcctgt
     2941 taaccaggag agtgatgtta ccgagacgaa agccgaagaa gttccttcgg atgtcgatga
     3001 aattgacgaa ctcatcaata tgctggaaac cgaatcagcc gaagtatcgt tcacggctcc
     3061 ggtgaagacg gaacctgagg tggctgttga gcccgagatt gaagttgata ttgaggagct
     3121 gaaaaaacaa gcgctccgcc cacgtggtga aatcgaagag caggaaacta tcaccgaggt
     3181 tcgttccgta gcgttagaag atggagaaga agtattcgtg gaagtggagt ccacgccgca
     3241 ggatcctctg gaggaaaggc tctcagcctt agaaaacgct ctgcgcccct atcgacggta
     3301 tatctacttt ggatttaccg ttttcggaat agtgctggcg cttatcgcct cggctgtctt
     3361 attcaacgtg tatcgccaga gtcgtccggc ggcgccaaca gaagtctcga atcttccata
     3421 tcccactgcc attcgtctgc cgggcggact gaactttacc cttggtaaag gatcgctgca
     3481 agctggccaa tggaatccac agggaccaga gtggcttcag gggacagagg tctgtcggtg
     3541 gatcgcgatt ccatggagca ctcaaataga agctgtgatt cgaacattaa acccggaaga
     3601 cccgatcgag ctcactatga gtaacaacga tcatttggta tacaaggtct attcaattca
     3661 acagttgacc aatgaagaat tgcaaaggtt gccttcggat tctccatgcc ttatgctcat
     3721 actagcaaag gcagagaccg agacgcgctg ggttcttaca gcgctaccct gaggtttcat
     3781 aaaaatcggc gcaccaagaa gagcctgctg aaggaaatgg ttccgttgac ttaatatcaa
     3841 aaaggttaca aaagaacgac aatatttggc tgcttttctg tttttcgtga agttttatct
     3901 acatatgggt gtcattgagg aacttgtaga tctccaacca ggtaactttt cgattatcgc
     3961 ttcgtcttgc attcactgaa taagcaggta tttgctacta ctgtgcatga tcaaatttct
     4021 caatggaatt cgattttggg atgcagagag ctggtgacaa aatctggtcg ccatcctcgc
     4081 attgagtgta gtcactggtg agcgatagcg aaaccggcct gcaatttggt gggtttttgc
     4141 tttctccaga ttgaatcgat ttctaggact caaaaattga tgtttatcaa gaaatttcat
     4201 cgacaagttc atttcgccgg atttcgtgcg caagccatcg ttgagttcgc gatcgctttg
     4261 ccgatcctca tggctctctt agtagggatt tttgaggtgg ggcgcatggt tttcatttat
     4321 tctgccgtta ccaacgccag tcgcgaagcc tctcgctatg gttcggctgt ggggcttgat
     4381 gatacaggta gctatgcaaa ataccaatac tgtgatggga ttcgagcgat ggcgcgacgg
     4441 tctgcttatt tcctgaacct ggcagatagt gatattctca tcgaatatga caatggtccc
     4501 agcacctctg tttttgatac gtgtaccagc aacgtcgata ccgccgtgca tgttaattcg
     4561 ggagctgagc cggatcgcgt gattgtcacc atttcaacgg actatagccc catgataaca
     4621 ctcattccca tcagctcgcg gactatcaca tcctccagcg cgcgaacaat cctcggtatt
     4681 cttgatctga attcaggaaa cagcgtggtc cctactacag gtccgacgaa cacgtcagca
     4741 ccaacggttc cagccacagc tcttccatct gatactccaa cagtgaaacc cacaggtcct
     4801 tccgatacac cgaccgcgac cgagccgggt ggtgatttaa ttacctttac gcccttgcca
     4861 agcagtacga tgacagatac tccgacgatt acgccgacat ttacagcaac ttttacacca
     4921 acgatgacct tcactcccac ggtgaccttt acaccgacca ccacttcgac gccggtgccg
     4981 ggttgtggca atattctggc cgggacaatc aatacggcca atagcagtaa cctgatgact
     5041 atgaccatca ccaatccgca tgacgcagtt actgtctcaa atattcatgt aacctggaac
     5101 agcaccacgg gagcgtctgg tagcaaaccc ttgacgttat taagtgtaag cctgggaact
     5161 accttctgga cgggtaacag cactgcagga agtggtttga cgatcacacc ttctacaacc
     5221 cttacgatcc cgggaaacaa tcgaatctca acgataattt tctcgtttga caacaaatat
     5281 cacaatccca tcgggaattc gatcacgatc aacctttcaa ctattggttg tgaatctaac
     5341 ccaattcaaa gaccctgatg cggttcgtta cctggaaaga gagagaaata tgaaaccaat
     5401 acatagcgaa cgcggacagg cattgattat ccttgctctt gcggcgattg gaattttcgg
     5461 aattgttggc cttgccatcg atgggagtgc caaattctca gaccgccgcc atgcacaaaa
     5521 tgccgctgat tctgctgcgc tggcgggcgc cctggccctg gttaacgaag atacgacatg
     5581 ggtacttgac gctttgaatc gtgccttaga gaa
//
LOCUS       DASTJP010000371         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_2546,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000371 DASTJP010000000
VERSION     DASTJP010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2546"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..508
                     /locus_tag="VFQ23_10980"
     CDS             74..508
                     /locus_tag="VFQ23_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907160.1"
                     /translation="MKKNILKSIGAVIAGFIAVFILSYGTDAILGIMSILPSGPLPLT
                     GSEFLVLTVLTYRNIYNVFGCYLTARLAPNHPMRHALILGVLGFLGSIGGALGGQGLA
                     PAWYSWMLVVLSLPCAWLGGKLYELNIKKRKVHESGTPVSQS"
     gene            667..1506
                     /locus_tag="VFQ23_10985"
     CDS             667..1506
                     /locus_tag="VFQ23_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9907161.1"
                     /translation="MTKDNFHSLQSTRPPDNTCAHLRIPVADTILDCNDSRGSGHPVI
                     FLNGAFGSQRDWKKVLSRLDSDYRTITYDERARGKSEKSSDYSFEGCLDDLSAVIAAT
                     GVQHPLLVGWSLGAAIAVRYTADHPGDVAGLLLIDGAYPIAMFTEAEKEKVRSAFQKM
                     RPFLPILARFGKAARMSADQAANVNIELDDILGKLGSSYENIHCPTYFICASKRSMGG
                     TDEQFRKMRASVVPLAARQQNIAIFKTLPCTHLEILSRYPDTIVAAINDLTRRSLSTN
                     NGK"
BASE COUNT          521 a          521 c          517 g          523 t
ORIGIN      
        1 gtcggatggc atcaatcaat ggacaagctt gctgccagct tacttaacct aattgtctga
       61 gtaaggataa atcatgaaaa aaaacattct taagagcatt ggagcggtga ttgcaggatt
      121 tattgccgtc ttcattctgt catacggtac cgatgccatt ctaggaataa tgagcatttt
      181 gccgagtggt ccactaccgc taactggctc ggaattcctg gtgctcaccg tgttgaccta
      241 ccgcaacatc tataacgtgt tcggatgtta cctcacagca agactggcac caaaccatcc
      301 gatgcgccac gcgctgattc taggcgtcct tggatttctt gggagcattg gcggcgcatt
      361 aggtgggcag ggacttgctc cagcctggta ttcatggatg cttgtcgtgc tttctctgcc
      421 ttgcgcctgg cttggcggca agctatatga attgaacatc aaaaaacgta aagtccatga
      481 atctgggacg cctgtgtctc aatcatagtt gagcctgttt acaaatgacg gttcgtttgg
      541 acgttgtgcg ataaggtcaa acgaaccgtc aaaagcatgc gaccgacgga tgggtatttt
      601 gccgatttag atgatatcgg tgccaaaaag aacggcttag aataaagttg atcgatcagt
      661 aacgatatga ccaaggacaa ttttcattct ttgcaatcaa cccggccgcc tgataacact
      721 tgtgcccatc ttcgaattcc tgttgcagat acaattctcg actgcaacga cagcagagga
      781 agcggtcatc ctgttatctt tctcaatggg gcgttcggca gccagcgtga ttggaaaaag
      841 gtactcagca ggctcgatag tgattatcgc accatcactt atgatgaacg tgctcgcggt
      901 aagtccgaaa aatcctctga ttactccttt gagggatgtt tggatgacct ctctgccgtt
      961 atcgctgcca ctggcgttca acatcccttg ctggtgggct ggtcccttgg ggccgctatc
     1021 gctgttcggt acaccgctga tcaccccggc gatgtcgcgg ggctactgct cattgatggt
     1081 gcatatccaa tcgccatgtt caccgaggct gagaaagaaa aagtgcggag cgcctttcaa
     1141 aagatgagac cgttcctgcc catcctggcc aggtttggga aagcagcgag aatgtctgca
     1201 gaccaggcag cgaatgttaa tatcgagctc gatgatattt taggcaagct aggctcctcg
     1261 tatgaaaaca ttcactgtcc gacctatttc atctgtgcat ccaagcgtag catgggaggt
     1321 acagatgaac agttccgtaa gatgcgcgct tctgttgtgc cgctcgctgc acggcaacaa
     1381 aacatcgcta tttttaagac cttaccctgc acccatttag aaatactttc caggtatccc
     1441 gatacgatcg tcgcagccat taacgacctg acccgacgct ccttatcaac gaacaacggg
     1501 aaatagtcaa tatccgatct accgattccg atggcccaac gctgaaaacg tgatgagggt
     1561 atattgcacg cgcctggccc aatatcggaa gaacggtcat tccgatcttc aaagttgtcg
     1621 gggcggcgaa accgagcgtt agtctcttcc tgaacggact aatcctaaac ggatcgccag
     1681 aatggccgcc tgtgttcgat cggtaacacc gagttttgag aaaatgatgc tgacgtaatt
     1741 cttcacggtg ccttccgaga ggaataagtc ccttgcaatc gcagaatttc gcaaaccctc
     1801 cgccagcaat cgaagcgcct cgcgctcacg tttgcagttc cagggggcag gtaaaacgac
     1861 ttaagggaca acctcagcct tgggatcgat agatacagga ataccagccc tggttccagc
     1921 aattggattg gtgtccatgt tattgcccgg ggccgcgact gaaaaaagca aattcctgtg
     1981 tgatcacgcc cagcgcagtg atggcaaact gcacacctgt aaagagtcga aaggttggca
     2041 ggatacccag ttccatttct cgcggggttt catgttttta tc
//
LOCUS       DASTJP010000372        11016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1152429, whole genome shotgun sequence.
ACCESSION   DASTJP010000372 DASTJP010000000
VERSION     DASTJP010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152429"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFQ23_10990"
     CDS             <1..1149
                     /locus_tag="VFQ23_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907162.1"
                     /translation="ALAGALALVNEDTAWVLQALNRALENGYNDNHLTNEVWVYRCHE
                     VDVTSPVDCGPYNGNPDYVQVAIESRVTTYFSRVIGIQETVNTVQAVTYAKEKGPFYD
                     GNLIVALNPNPCSGQSGNIVLGGNSDVTLSGGGAFVNSGGSDCGMEQGGSCPTFQNGG
                     AIGSPGDGNINLDGCANVPAPTYGADPYAFPPEMPAEPAECTSPMGTWSSNGSTQITT
                     LSPGRYNEFPPKSTQSLTVYDTVIMNPGIYCVNDVVKLSDHHLDLYGHDVTIFIREGY
                     DFDIQGGRIRLDAPDTGDYAGYLLIVDSNFTGQVPNCIINGDSENEYTGTIFAPYCDI
                     TINGGNDTTTYNAQMIGYTVSILGNAHTNLFYDTNNTAQSTPKIGLMR"
     gene            1243..2529
                     /locus_tag="VFQ23_10995"
     CDS             1243..2529
                     /locus_tag="VFQ23_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET9907163.1"
                     /translation="MSKKLAAQAVPGITDPDLLLPRVLKHLLELTEETPPLGERYEEL
                     LRGLIFQAYEIANGDLGQGEREFLDEVFDNIAGYGPMEEFFDDPQISEIMVNGPNQIF
                     IEKNGKLILTDARYESDLQLRFAINHIINPFGRYVNYKHPMVDAHLKDGSRINVVIPP
                     VAQQGSCLSIRRFLKDKLAVQDLIDKDALTQGMADFVAVCVAARLNVIVAGNTSSGKT
                     TFLNILARSIPDHERIVTIEDSVELQMMQTHKVSLEARPPDYNGEGQVTIRDLVKNAL
                     RMRPDRIVVGEIRGGEALDMLQAMNTGHDGSMTTVHSNSPRDTLSRLETMTLMAGFEL
                     PVLAIRKQISAALDLIIYLTRFPDGTRKVTQISEVGGMESDVITLTDIFLFQQNGTSP
                     EGKPLGQFRPTGLRPMFAPKLEAMGFRLDRQMFMTR"
     gene            complement(2593..3525)
                     /locus_tag="VFQ23_11000"
     CDS             complement(2593..3525)
                     /locus_tag="VFQ23_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9907164.1"
                     /translation="MTEVSTETRFLHAIEMGLGAWQWGDRIVWQYGHNYGPEEVRQAF
                     QVSANEGIRFVDTAEIYGNGRSERLLGQFLKETDQPILIATKFFPLPWRFTKGALPRA
                     LKGSLARMGVDSVDLYQIHWPSLVMSTDSLMEGLVECVKSGITRTVGVSNYAQSRMLA
                     AYSSLARHNIPLASNQVHYSLLNREVEKNGTLARCKELGIRLIAYSPIEKGLLSGKYS
                     ADNPPPGSRAAGSSAILPKIGPLLKLMTEIGQDHGGKSNTQVALNWTICKGTLPIPGA
                     KNAEQAQQNAGALGWKLTDEEVARLDETSDFILK"
     gene            3678..4418
                     /gene="rnc"
                     /locus_tag="VFQ23_11005"
     CDS             3678..4418
                     /gene="rnc"
                     /locus_tag="VFQ23_11005"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET9907165.1"
                     /translation="MALPEFREAPVTSLFSPRDFEPASDLSRRLGLPFSNLALLTRAL
                     THRSFVNENPSAIEDNERLEFLGDAVLDFIVGAWVYHRFPEMPEGDLTKMRSALVRND
                     QLADFARHLNLGVALRLGRGEFASGGHQRDGLLGSAFEALVGALYLDSGLNSVELFVH
                     PLLEQARDAIVNKVNDPKSQLQEWAQSQKLGAPRYKTVATSGPDHAREFDVQVEIGGQ
                     VVGQGHGKNKHAAEHVAAKHALKTLGIE"
     gene            4438..8049
                     /gene="smc"
                     /locus_tag="VFQ23_11010"
     CDS             4438..8049
                     /gene="smc"
                     /locus_tag="VFQ23_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET9907166.1"
                     /translation="MPLRLKSLELQGYKTFASKTTFEFASGITAIVGPNGSGKSNIAD
                     SLRWVLGEQSYALLRGKKTEDMIFNGSEHRPRAGMASAHILFDNSNGWLPVDFTEVGM
                     TRRAFRDGHNEYLLNDQHVRLRDLNELLAASGLSERTYTILGQGLVDASLALKADERR
                     RLFEEAAGVGLYRVRREEALKRLDNTTRNLERVLDIMSELEPRLRSLERQAKRAIEFT
                     RAQSNLKVILREWYGYHWHRAQQELTEAREHVKAQDARVLQAREVHQKAQEEYNKFRE
                     RFTGTRSQLNTWHHQSAELHNQREEVSRALAVLEERRRSLHSAQSSLLADKDRTSDEE
                     RIARERLIEADQDAGRLQTEYDEARRQFSEAQQSLQARQTERTLVEEKLQAARAEIEK
                     RAAQRAEYQARLEELEARLNGFQVRMESTRELVESAEVTVREAGEKHGQARKVREDLE
                     RALIKTEEEAENARREIERIENERRAKADQRSNVLAEYTRLKIQREVLEQAEQSLAGY
                     AEGARFLLDAVRQSRLKGARGALSASLDVPAELEIAVTAALGDTLDAILLDASEVDEA
                     LILLESDDSGRAALLPLGNNSRSHLTPPNDEDSLGIASELVHAPDELRDAVHLILGQT
                     LIVRDRKAARRLIENMPTHARIVTLRGEVFRGDGLIIAGKSTSMSGSALSRPRQKREL
                     TESLGVLTGEIETLNSEVDSLSAMITNAQRDVGQAEAAVRESRLRLEEAQATEQQTRF
                     EAESVRRQWEWQKSQFEQLQSEVREAESVRQNLMQSQSEMENQSAESMSHVHTLSEQL
                     GGMDLMEAQEQASYWGTRVAVVERAVEDVLAKKEERVKEAARFDSRNTELAERLQETE
                     TSLSNLEIEKSALRERETVLHTQIEELRVKMDPAEKDLESAEQEETRLQDAEANTQRS
                     LATAERLAGQQQLEQLRKQEGLDNLRQRITDDFGLVMFEYAEDIAGPVPLPFEGMVEE
                     LPVVTEISPELESQLTHNRVQLRRMGAINPEAKQEFDTESERYSFMKTQVDDLRKAEE
                     DIKQVVAELDELTRREFSKTFDAVDKQFRAMFTRLFGGGSARLSLTDPDNLVETGIEI
                     EARLPGRREQGLALLSGGERSLTAVALVFALLKVSPTPVCVMDEVDAMLDEANVGRFR
                     DLLQELSKETQFIIITHNRNTVQAADIIYGITMGRDSASQIISLRLDQVTDDMLKQA"
     gene            complement(8126..9439)
                     /locus_tag="VFQ23_11015"
     CDS             complement(8126..9439)
                     /locus_tag="VFQ23_11015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9907167.1"
                     /translation="MAKPGPTPKIVTKKHIARLERERQQVRLIRAIAIGGIVAVVLLL
                     AYGYLNLNLFQLQRPVAEVNGEVITTREWQERVRFQRANLLNLYNTYIFYQQSFGMDT
                     TQQQQEVMFYLQTPEALGQQVIDQMIDEELIRQEAEKRGITVSAEEIQKEIENEYGFF
                     PNGTPTPTVTPTEFIALTLSAAQLTIYPSTLTPTEAPTSTAAPTSTPDPAVTQTPTST
                     VAPPTPTFVQDLPTATATPFTEEGFNEQYASTLDEFKSYGVTEATLRSVYENRLLREK
                     LLEEIAADVPRTEEQVWARHILVEDDETSGIVRSLLLSGKDFGEVAKQYSKDTGSGAL
                     GGDLGWAPRSKYVTEFADAAFSQEIGAIGEPVRSQFGIHIIQVLDRQERPLDATTYEQ
                     SREAALTDWLTQTRAEATEAGTLITYEGWSAFVPTEPSALNPQTP"
     gene            complement(9520..9804)
                     /gene="rpsR"
                     /locus_tag="VFQ23_11020"
     CDS             complement(9520..9804)
                     /gene="rpsR"
                     /locus_tag="VFQ23_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET9907168.1"
                     /translation="MAMDRRPQEGTEGGGERRFFAKPKFCQFCADKTLTIDYKKTDIL
                     RKYVTEDGKIRPRRQTGACARHQRAVAAAVKQARHIALLPFTGRHLEDTR"
     gene            complement(9940..10371)
                     /locus_tag="VFQ23_11025"
     CDS             complement(9940..10371)
                     /locus_tag="VFQ23_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET9907169.1"
                     /translation="MSRGLNKVQIIGHLGRDPEMRYTPSGRPVTTFTVAVSRQWNTGD
                     GERHNETEWFNVVAWGNLAEICKQYLNKGQQVYIEGRLQTRRWDDKEGVKHTSVEVVA
                     NEMMMVGDRADRRDQNSQAPHPMEAGPNDNDLTNNEDEFPF"
     gene            complement(10420..10701)
                     /gene="rpsF"
                     /locus_tag="VFQ23_11030"
     CDS             complement(10420..10701)
                     /gene="rpsF"
                     /locus_tag="VFQ23_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET9907170.1"
                     /translation="MRKYELVCIVQPELDETAFKGVVDRVSGWVTETGGSVDKVDVWG
                     RRKLAYIIHKQREGQYVLMNITLDPKATGELERNIRYLEPVLRHMLSAV"
BASE COUNT         2724 a         2662 c         2936 g         2694 t
ORIGIN      
        1 gctctagcgg gtgccctggc actggtgaac gaagatacgg catgggtgct tcaggctttg
       61 aatcgtgccc tagagaacgg ttacaacgac aaccatctta ccaacgaagt ttgggtttac
      121 cgttgtcatg aggtggatgt cacctcaccg gttgattgtg gtccttacaa tggcaatcct
      181 gattatgttc aggtggcaat tgagtcgcgg gtcaccactt atttttcgcg agtcattggc
      241 attcaggaga ccgtcaatac ggttcaggct gttacatatg caaaggaaaa gggaccgttt
      301 tatgatggga atctgatcgt agcgctaaat cctaaccctt gctcaggtca atcgggcaat
      361 atcgttttgg gtggtaatag cgatgtcacc ctctctggag gaggagcgtt tgtcaattca
      421 ggcggaagtg actgcggaat ggaacagggt gggagctgcc cgacctttca aaatggcgga
      481 gcaattggtt cacctggcga cggcaatata aacctggatg gttgcgcaaa tgttccggcg
      541 ccaacctacg gcgcggatcc ttatgcgttc ccaccagaga tgcccgctga gccagctgaa
      601 tgcacatccc cgatggggac ctggtcaagc aatgggtcaa cccaaatcac aacactgtcc
      661 ccgggcagat acaatgagtt tccaccgaaa tctacccagt ctcttacggt atacgatact
      721 gtaatcatga accctggcat ctattgtgta aacgacgtcg tgaagcttag tgatcaccac
      781 ctggatcttt atggtcacga tgtcacaatt tttattcgtg aaggctacga ttttgatatt
      841 cagggcggtc gaattagact cgacgctcct gatactggcg actacgccgg atatctcctg
      901 attgtggact cgaactttac cggtcaagta ccgaactgta ttattaatgg tgattcagaa
      961 aatgaatata caggaaccat atttgcccct tattgcgata tcacgattaa cggtgggaac
     1021 gataccacca cctataatgc acagatgatt ggatacaccg tctccattct gggtaacgcg
     1081 catacgaatc tattttatga taccaacaat actgcgcaaa gtactcccaa aatcggtctg
     1141 atgcgataga ttatttcata agggcgagcc aggaaaacga agctcgcctt ctttattatt
     1201 tcaaaaccct gaacaaattc aactcaagct ctggagaaca ctatgtcgaa gaagctagct
     1261 gcgcaagccg tgcccgggat cacagatcct gatttgctgc tgccgagagt tctaaagcac
     1321 ctcctcgaac taactgaaga aactcctcct ttgggagagc ggtatgaaga gctactaagg
     1381 ggattaatct ttcaagctta tgagatcgca aacggcgacc ttggacaggg cgagagagag
     1441 ttcctggacg aagttttcga taatatagca ggctacggtc cgatggaaga gttcttcgat
     1501 gatccacaga tcagtgagat catggtcaac ggtccaaatc aaatctttat tgaaaaaaat
     1561 ggaaagctaa tcctaaccga tgcccgatat gagagcgatc ttcagctgcg atttgcaatt
     1621 aatcatatta ttaatccatt tgggcggtat gtgaattaca aacacccgat ggtcgatgcg
     1681 cacctgaagg atggctcgcg tatcaacgta gtgattcctc ctgtagccca acaaggctct
     1741 tgtctttcca ttcgtcgatt tttgaaggat aaactggctg ttcaggatct cattgataaa
     1801 gatgcactta cccaaggcat ggccgatttt gtggctgttt gtgtagctgc gcggctcaac
     1861 gtcattgtgg ccggcaacac cagctccggg aaaacaactt ttcttaacat tcttgcgcgc
     1921 tcaattcccg accatgaacg gatcgtgacc atcgaagatt cggtagagtt gcaaatgatg
     1981 caaactcata aagtttcact ggaagccagg ccgcctgatt acaacggcga ggggcaggtc
     2041 acaattcgcg atctcgtaaa gaatgcgctc cgcatgcgcc cggatcggat tgtggtcggt
     2101 gaaatccgtg gcggcgaagc ccttgatatg cttcaagcca tgaatacggg tcacgatggc
     2161 tcgatgacaa ccgtgcactc caattcaccg cgtgacacac tctctcgtct ggaaaccatg
     2221 acgttgatgg ctggcttcga acttcccgtg ctggccattc gtaaacagat ttcagcagct
     2281 ctcgatttga tcatctatct aactcgtttt ccagatggaa cacgaaaggt aactcaaatt
     2341 tctgaggtgg gtggcatgga gtcggatgta atcactctga ccgacatttt tctttttcag
     2401 cagaatggaa ccagcccaga aggaaagcca cttgggcaat tccgtcccac tggtttgcga
     2461 cccatgttcg cacccaaact ggaagcaatg ggcttccgtc tggatcgtca gatgttcatg
     2521 actcgataat cagagtcgga aatgcccagc aaactttgct gggcattttc attacaggac
     2581 atgacactca actcacttta gaataaagtc actggtttca tccagcctcg cgacttcctc
     2641 atcggttaac ttccagccga gggcgccagc attttgctgt gcttgttctg cattcttcgc
     2701 tccgggaatg ggtagcgtgc ctttgcagat cgtccaattc agcgccactt gcgtattgga
     2761 ctttcctccg tggtcttgtc cgatttcagt catcagcttg agaagtggcc cgattttagg
     2821 caggatggct gaggaaccag ccgcgcgcga accgggcggc ggattgtcgg cgctgtactt
     2881 gccggaaagc aatcccttct caatggggct gtaagcgatc agccgaattc caagttcttt
     2941 gcaacgcgcc aaggtgccat tcttttccac ctcacgattt aataaactat agtgcacctg
     3001 gttggatgct agagggatat tatgacgtgc aagcgaagaa taggctgcca gcattcggct
     3061 ttgcgcatag ttcgaaacgc caacggtccg cgttatgcca ctctttacac actctaccaa
     3121 accttccatc aagctatccg tactcatcac gaggctgggc caatggatct gatataaatc
     3181 tacgctgtca acgcccatgc gagcaaggct acccttcagt gcccgcggca gagcgccttt
     3241 cgtaaacctc cagggaagag ggaaaaattt cgtcgcgatc aaaatcggtt gatcggtctc
     3301 ttttagaaac tgcccaagga gacgttctga gcgtccgttg ccatagattt ctgcagtatc
     3361 aacaaaacgt attccttcgt tcgcagaaac ttgaaatgcc tgacgcactt cctctggacc
     3421 atagttgtgc ccgtattgcc acacgatccg gtcaccccac tgccacgcac ctaatcccat
     3481 ttcaattgca tgtaaaaagc gggtttccgt gcttacttct gtcaaattgg tcctccagac
     3541 ggaacaaatc gcattgtatc ttaaaatatg attgtataat agcgcgtgaa ttgaattcga
     3601 ttagaattca acctacttaa tgcattggaa ctgatggaac gcgatgccct ccgatctatc
     3661 tccgaaagga gaggggtatg gcgctccctg agttccggga ggctcctgtg acatctctgt
     3721 tctcaccgcg agacttcgaa ccagcttcgg acctaagtcg gcggctgggt ttgccctttt
     3781 caaatttggc gttgctaacg cgtgcactga ctcatcgttc cttcgtgaac gaaaatccca
     3841 gcgcgatcga agacaacgaa cggctcgagt tcctgggtga cgccgtgctt gattttatcg
     3901 ttggcgcgtg ggtatatcat cgtttccctg aaatgccaga aggcgatcta acaaaaatgc
     3961 gatctgcgct ggtgcgcaat gatcagctgg cagatttcgc gcgacatcta aatctgggtg
     4021 tggccttgcg attggggcgc ggtgaatttg cctctggagg ccatcagcgt gatggcttgc
     4081 ttggatctgc atttgaggcg ttggtcggtg ccttgtatct ggattcagga ttgaattcgg
     4141 tagaattatt tgtacatcca ctattagaac aggcgcgtga cgcgatcgtt aataaggtga
     4201 atgatccaaa gagtcaatta caggaatggg cgcagtcgca aaagctggga gcgccgcgtt
     4261 ataaaacggt cgcgacgagc ggtcccgacc acgccaggga attcgacgtg caagtggaga
     4321 tcggtgggca ggtggtgggg cagggccatg gtaaaaacaa acatgctgcg gaacatgtag
     4381 ccgcgaagca tgcactgaag acgttgggaa tcgaataatc aaaattgaga ctatgaaatg
     4441 cctttaagac ttaaatccct tgaattgcaa ggctataaaa ctttcgcctc aaaaaccaca
     4501 tttgagtttg cgagcggcat caccgccatc gtcgggccaa acggttctgg caaatcgaac
     4561 attgccgatt cactgcggtg ggtattgggg gaacaatcgt atgcactttt gcgcggcaag
     4621 aagactgaag acatgatctt caacggttcg gagcaccgac cgcgcgccgg gatggcttcg
     4681 gcacatatcc tcttcgacaa tagcaatggc tggctcccgg tggacttcac cgaagtcggc
     4741 atgacgcgcc gtgcttttcg cgacggtcac aatgaatatt tgttgaatga tcaacacgtg
     4801 cgattgcgcg atttgaatga attgcttgct gcttcgggct tatcagagcg cacgtatacc
     4861 attcttggtc aaggtctcgt cgacgcctcc cttgccttga aagcggatga gcggcggcgt
     4921 ttgtttgaag aagccgcggg tgtgggattg tatcgcgttc gccgcgagga agcgctcaaa
     4981 agactcgata acacgacacg taaccttgaa cgtgttcttg atattatgtc tgagctcgaa
     5041 ccgcgtttac gcagcctgga acgtcaggca aagagggcga tcgaatttac tcgcgctcag
     5101 tccaatctaa aagtgatcct gcgcgaatgg tacgggtatc actggcatcg tgcccagcag
     5161 gaattaaccg aagcgcgcga acatgtgaag gcgcaggatg ctcgcgttct ccaggcccgc
     5221 gaggtccatc aaaaggcaca ggaagaatac aacaaattcc gagagcggtt cacgggcacg
     5281 cgttcgcaac tgaacacatg gcatcatcaa tcggcagaat tgcacaacca gcgagaagag
     5341 gtgagtcgtg cgcttgcggt gcttgaagaa cggcggcgat ccttgcattc tgcgcaatcc
     5401 tctctgcttg ccgataaaga ccgcacttcc gatgaagaac gaatcgcgcg tgagcggtta
     5461 atcgaagcag accaggacgc agggcggtta cagacagagt acgatgaggc aaggaggcaa
     5521 ttttccgagg cccagcagtc cctgcaagcc aggcaaaccg agcgcaccct cgtggaggaa
     5581 aaacttcaag ccgcgcgcgc cgaaattgaa aagcgagcag cgcagcgcgc tgaatatcag
     5641 gcacgtttag aggaattgga agcgcgcctg aatggtttcc aagtgcgaat ggaaagcacc
     5701 agggagttag ttgaatctgc agaggtaact gtgcgtgaag cgggtgaaaa gcacgggcag
     5761 gctcgcaaag tgcgcgagga tcttgaaaga gcattaataa agaccgaaga agaagcagaa
     5821 aacgctcgaa gagaaatcga aagaatcgaa aatgagcgca gggcaaaagc cgatcagcgt
     5881 tcgaatgtac tggcagaata cacccgtcta aaaattcaac gggaagtgct ggaacaggca
     5941 gagcaatccc tcgctggcta tgctgagggt gcacgatttc tcctggatgc cgtacgtcag
     6001 tcacgactga agggtgcgcg gggtgccctg agcgcctcac tggatgtacc tgcggagttg
     6061 gaaatcgctg tgactgccgc attgggtgat acgcttgatg caatcctgct ggatgccagt
     6121 gaggtggatg aagcgctgat cttacttgag tctgatgatt cggggcgcgc agcattgctt
     6181 ccgcttggga ataattcccg gtcgcatctc acgcctccga atgacgaaga tagcctgggc
     6241 attgcgtcag agctggttca cgccccggat gaattacggg atgcagtgca tctcatcctg
     6301 gggcaaactc tgatcgtccg agatcgtaaa gccgcgcggc gtctgatcga aaatatgcca
     6361 acgcacgcgc gcatcgtgac gttgcgcggc gaggtgttcc gtggggatgg gctcatcatt
     6421 gccgggaaat ccacttcgat gagtggctca gcattgagtc gtccgcgcca gaaacgcgag
     6481 ttgacggaat ctcttggggt actaaccggg gagattgaaa ccttgaatag cgaggtcgat
     6541 tcgctttctg ccatgatcac aaatgcgcag cgcgacgttg ggcaggcaga agctgcagta
     6601 cgagagtcac ggcttcggct ggaagaggct caggcaactg aacagcagac ccggttcgag
     6661 gctgaatcgg ttcgccggca gtgggagtgg cagaagagtc aatttgagca actgcaatct
     6721 gaagtccggg aggcagaatc ggtccgtcag aatttgatgc aatcccagtc cgagatggaa
     6781 aatcagtctg cagagtcgat gagtcatgtg catactttgt cggaacaact gggtggaatg
     6841 gatttgatgg aagcacagga acaggcctct tactggggaa cccgcgtggc agtggtggaa
     6901 cgtgccgttg aagatgtgct agccaaaaaa gaagagcggg tgaaagaagc cgcaaggttc
     6961 gattcaagga acaccgaact ggcggagcgc ctgcaggaaa ctgaaacgtc attaagtaat
     7021 ttggaaattg agaaatctgc attgcgagag cgggagacgg tactacacac tcaaattgaa
     7081 gaattacgcg tgaaaatgga tccggccgaa aaggatcttg agagtgcaga acaggaagag
     7141 acgcgcttgc aggatgccga agcgaacaca caacgatctc ttgcaacggc agagcgattg
     7201 gcgggtcaac agcaattgga gcagttaaga aagcaggaag gattagataa ccttcgccag
     7261 cgaattacag acgatttcgg tttggtgatg tttgagtatg cagaagatat cgctggaccg
     7321 gtaccactgc cattcgaagg aatggttgag gagttaccgg tggtcacaga aatttcaccg
     7381 gagctcgagt cgcagcttac ccataatcgt gttcaattgc gtcgtatggg agctatcaac
     7441 ccggaagcaa aacaggagtt cgataccgag tctgagcgtt attcgtttat gaaaacccag
     7501 gtggatgatt tgcgtaaagc cgaggaggat atcaaacagg tcgtcgcaga gctcgatgaa
     7561 ttgactcgac gcgaattctc aaaaacgttc gatgccgtgg ataaacaatt ccgagccatg
     7621 ttcacgaggt tgttcggcgg aggatctgcg cggctctcac tcacggatcc ggataatctg
     7681 gtggaaaccg gaatcgagat tgaggcgcgg ctgccgggac gccgcgaaca agggctggcc
     7741 ttactttctg gaggagaacg gagcctgact gccgttgcac tggtatttgc attgcttaag
     7801 gtttcgccta ccccagtctg cgtaatggac gaagtggatg cgatgctgga cgaagccaat
     7861 gtggggcggt ttcgtgatct gttgcaggag ttaagtaaag agactcaatt catcatcatc
     7921 acccataacc gcaatacggt tcaagccgca gatatcatct acggtattac gatgggccgc
     7981 gattcggcga gtcagatcat cagcttgcgg ttagatcaag tgactgatga catgctcaaa
     8041 caggcgtgac catcaacgtt gttatgaaat aaaattgccc gccttatgaa tggcgggcaa
     8101 tttttgtttt attttctaaa ccggtctacg gcgtttgtgg atttaatgca gagggttcag
     8161 taggaacaaa ggcactccaa ccttcatagg ttatgagggt acctgcttct gttgcctccg
     8221 cgcgagtttg cgtaagccaa tcggtaagcg cagcttcgcg actttgctca taggttgttg
     8281 catccagagg gcgctcttgg cgatcgagta cctggatgat gtggatacca aattgcgacc
     8341 ggacgggttc gccaatcgcg ccgatttcct ggctgaaggc ggcatctgcg aattcagtca
     8401 catacttact gcgtggagcc cagcccaggt cgccaccaag agcaccggag ccggtatcct
     8461 tggaatattg tttggcaacc tcgccgaaat ctttcccgct gagcaagagg gatcgaacaa
     8521 ttccggacgt ttcatcatcc tctaccagaa tatgccgtgc ccatacctgt tcctccgttc
     8581 gaggaacatc cgctgcaatt tcctccaaca gtttttcgcg aagtagcctg ttctcgtata
     8641 cggagcgcaa cgttgcttca gttacgccat acgacttgaa ctcatccagc gtagaggcat
     8701 attgttcgtt aaagccttcc tctgtgaacg gtgtagctgt agctgtcggc aggtcctgaa
     8761 cgaatgtagg cgtggggggc gcgaccgtgg aagtcggtgt ttgcgtcaca gcgggatcag
     8821 gtgtacttgt gggtgccgct gtggaggtcg gtgcttccgt aggcgtcagg gttgaaggat
     8881 aaatggtcaa ttgggctgca gaaagagtga gggctataaa ttcagtcggc gtaacggtag
     8941 gtgtaggtgt tccattcgga aagaaaccgt actcattttc aatttccttt tggatttcct
     9001 ccgcgctaac cgtgatgccc cttttttcgg cttcctggcg aatcaattct tcatcaatca
     9061 tctggtcgat gacttgttgc cccagggctt caggtgtctg caggtagaac atcacttcct
     9121 gttgctgctg ggtggtgtcc attccaaaac tctgctggta aaaaatgtac gtattgtaaa
     9181 ggttgagcag gtttgctcgc tgaaagcgca cacgttcctg ccattcacga gttgtgatga
     9241 cttcgccgtt gacttctgca accggtcttt gtaattgaaa taaatttaga ttcaaataac
     9301 catacgccag aaggagaaca accgcaacaa tgcctccaat ggcgatggcc cgaatcagtc
     9361 taacttgttg tcgctcccgt tccaggcgag cgatatgttt ttttgtgaca atctttggtg
     9421 ttgggcctgg tttagccatt caatttctcc atgacgcaaa gacggggcat ggcgcgaatc
     9481 attgtgcgcg ttccatgccc cgtacctgcg ttcgggggat cagcgcgtat cttccagatg
     9541 ccgtccggta aacggcaata aggcgatatg acgggcttgc ttcacagcag cagcgaccgc
     9601 gcgctggtgt cttgcacagg ctcctgtctg gcggcgtgga cggattttgc cgtcttctgt
     9661 cacgtacttc cgcaggatat ccgtcttttt gtagtcaatt gtcagagtct tatcagcaca
     9721 gaactgacaa aatttgggct tggcaaagaa gcggcgttct ccgcctcctt ctgtaccttc
     9781 ttgtggtctt cggtccattg ccatttgtta ctccagttta tttttctagg cagaccctag
     9841 agacttgtca tcggaacaag aagtgatttc taacgaaagc gcacccagat cttgcagcag
     9901 tgctattcat aggaaacccg tagggtctga ggtaatttct taaaacggga attcatcctc
     9961 gttatttgtt aagtcattgt cgttgggacc tgcttccatc ggatgaggtg cctggctgtt
    10021 ttggtcacgg cggtctgccc gatcaccaac catcatcatc tcattggcaa cgacttccac
    10081 gctggtgtgt tttacacctt ctttatcgtc ccagcgacgg gtttgtaatc gtccttcgat
    10141 ataaacctgc tggcctttgt tcagatattg tttacagatc tctgccaggt tgccccatgc
    10201 aaccacattg aaccactccg tctcattatg ccgctcgcca tctccggtgt tccattgacg
    10261 gcttacggcg acagtaaacg tcgtgacggg ccgcccggac ggggtataac gcatttcggg
    10321 gtctcgcccc aggtgaccaa taatctgaac tttgttcaat cctcggctca tatcaatctc
    10381 cttaatacaa gtaaagcggg aaattcaccg aatcttttat tacacagccg ataacatgtg
    10441 gcgcaggacg ggttcgagat agcggatatt tcgctccaat tcgccagtgg cttttgggtc
    10501 aagtgtgatg ttcatcagca cgtattgccc ttcgcgttgt ttgtggatga tgtaagccaa
    10561 ttttcgacgt ccccaaacgt ccactttgtc tacgcttccg cctgtttctg ttacccagcc
    10621 agagacacgg tccactacac ctttaaatgc agtttcatcc agttctggct ggacaatgca
    10681 aacgagttca tatttgcgca tgagggattc ctcctttcca tgggattgct ccagttcaaa
    10741 ccgcaggtct gccaggagcg gaaaggcacg tattagacgt acctgttgca aacgggctgt
    10801 agtttatcac gaattccaga attgcggagg gttataattc cggtagaaaa ggaagcaagt
    10861 aagcacgtaa acaggagaat tatgttcgac ctcgcggatg atgaaatttt gcagttaaca
    10921 aaggaacacg tattttttac ctggtcagcc cagtcgaagg taaatcccat tgcggtgaag
    10981 cgagcccagg gagtttattt ttgggataca gacgac
//
LOCUS       DASTJP010000373         6540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1414237, whole genome shotgun sequence.
ACCESSION   DASTJP010000373 DASTJP010000000
VERSION     DASTJP010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414237"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFQ23_11035"
     CDS             complement(<1..467)
                     /locus_tag="VFQ23_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907171.1"
                     /translation="MPHPRVSKKPVGRLTRGKTASNRLRRVDNFILLYEPSLLTRTEG
                     LFADSLFVDLGYGFDARTTLESALRFRRINPGLQVLGVEIDKERVEAALPYADKKTFF
                     RLGGFNLPLKDGEHVRLIRAFNVLRQYEEKDFAPAYDRLAAYVLPHGLMIEGTS"
     gene            complement(528..4778)
                     /gene="rpoC"
                     /locus_tag="VFQ23_11040"
     CDS             complement(528..4778)
                     /gene="rpoC"
                     /locus_tag="VFQ23_11040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET9907172.1"
                     /translation="METKGLTALRISLASPQTIMSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPQRDWQCYCGKYKNPRYKGIVCDKCGVEVTRAAVRRERMGHIALATPVAH
                     IWYTRRIPSYLGMLLDISRRNLDRVLYFAQYIVTYVDEEARNKALKRIEDEISVTERE
                     QAAGINAKIVEAKTERDRTLNEINQKRASLEQGYDEVIAEKLDPVIKEGQKLEKLLQG
                     QMGEHAKKAVIFELTDEKILDAGDKVAAKHLTQVQKIVQKNLEAVENELKDQRAKELE
                     DLKMDAGRVKADAEMKMEGLRSQLEDQTSVSSNQSSRMRDELMELRPFTFISEIRYRE
                     LKQRWGQVFRADMGAEAFYDILERLDLDKLSVELWHEVRTTKSKQKRKKATTRLKVVE
                     AFRRSGNRPEWMILTVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPDVIIRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVVGPQLKLYQCGLPKSMALELYRPFVIARLVQNNYAANVKGARR
                     LIERNRPEVWEALEGVIGERPVLLNRAPTLHRLGIQAFEPILIEGSAIQLHPLVTTAF
                     NADFDGDQMAVHVPLSQKAVQEARDLMLASKNLLKPADGEPIISPSKDMVLGVYYLTM
                     AQKANHKGDGRAFADIDEVEMAYVLGQVEVHSEVKLRAKTWYDEKGDRLPEAETRIID
                     TTVGRVLFNRILPEEVQFVNEKLDKGGVKDLIADVYEVCGQEKTTEVADQIKALGFEY
                     AMRSGTTLAVADISIPPERKGIIDEALKQVELVQRDFRRGLLTEQEKNEREIEIWQGT
                     TDRVADAVKKHMDPDGNLSTMASSGATKGGFSTISQLAGMRGLMADPSGRIIPMPIRS
                     NFREGLTAQEYFISTHGARKGLADTALRTADAGYLTRRLVDIAQDIIINEHDCGTHDG
                     VYIRRRDDVAGQALSSRLYSRLLAENVIDPKTGEVIGEYNDIINQELARKIAATGVEE
                     VKVRSALTCELQHGICSKCYGLDLGRGAMVDLGSAVGIVAAQSIGEPGTQLTLRTFHT
                     GGVAAGGADITTGLPRVEELFEARKQPKGEAVVAEISGTVRVNQSEKYADLREITIEH
                     SELVSDEYSIPEEWKFAVKDEAEVKAGDVLATQDDAKIVAQHGGRVRVEKKERKVIVS
                     YDQREEVSYEIPTTSRLLIKSNEKIEAGQPLTEGSLNPHRVLKIQGREACQMYLMTEV
                     QKVYRSQGQNIHDKHFEVIIRKMMSKIQVTRPGDTKFLPGDAVDRLELRKINEQLLAD
                     GKSPAKFSEVLLGVTKASLSTDSFLSASSFQHTIKVLAGAAIGSTTDPLFGLKENVII
                     GKLIPAGTGFIHGRFANEEGNGNASQWTEISDATAADD"
     gene            complement(4992..6395)
                     /locus_tag="VFQ23_11045"
     CDS             complement(4992..6395)
                     /locus_tag="VFQ23_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9907173.1"
                     /translation="MTTPDPDFVSFLRAYPAYPSTKIIDELRAVEYARLDLGGHIYLD
                     YTGGGLYADSQLRQHNRLLAEHVFGNPHSSNPTSHAATQMVEHAREYVLKFFNADPDE
                     YLAIFTTNASGALKLVGESYPFRGGRYLLTFDNHNSVNGIREFAHARGAEVTYIPVAL
                     PDMRVDESQLERELARTSKNGHNLFAYPAQSNFSSVQHPLDWIEKAHQKGWDVLLDAA
                     AYVPTCQLDLSQVKPDFVPISFYKMFGYPTGLGALIARKEALGQLQRPWFAGGTITVA
                     SVMGDKYYMADGASAFEDGTIDYLNIPAVEIGLKHIESIGYDVIHERVHSLTGWLLDN
                     LTSMKHSTGIQLTRVFGPVTTDGRGGAVTVNFYDKDNQLIYHRLIEQKANEANISLRT
                     GCFCNPGAGEVAMGVSRVELDVCFTSPGNEERLSQDDFRMCFDGKSMGAVRISVGMVT
                     NFNDVQNVLSFARGFLS"
BASE COUNT         1524 a         1780 c         1644 g         1592 t
ORIGIN      
        1 gacgtccctt cgatcattaa tccatggggg aggacatacg ctgcaagtct atcataggca
       61 ggagcaaaat ccttttcttc atattgacgt aagacattga aggcgcggat caaacggaca
      121 tgttcgccat ctttcaaagg aagattgaac ccacccaggc gaaaaaatgt ctttttatct
      181 gcgtacggca gggcagcctc tacacgctct ttgtcaattt caacacccag cacctgcaaa
      241 ccagggttta tgcgcctgaa acgcaacgcg ctttccagcg tggtccgcgc gtcaaagccg
      301 tagcccaggt caacgaacaa cgaatcggca aacaggccct cggtccgtgt gagaagcgac
      361 ggctcataga gcaggatgaa attgtcaacg cggcggagtc gattcgaagc tgtcttaccc
      421 cgcgttagcc gtccgacagg cttttttgaa acacgagggt ggggcatata cagtatttta
      481 aatcaaaaag gcgaccgatc ttcggtcgcc ttttattaat tctggggtta gtcgtctgct
      541 gcggtggcgt ctgaaatttc tgtccactgc gaggcattgc cattgccttc ctcgttggcg
      601 aaacgcccgt ggatgaaacc ggtgcctgcc ggaatcagct tgccgatgat cacattctct
      661 ttcagcccga agagcggatc ggtcgtggaa ccgattgccg cgcccgccag caccttgatg
      721 gtgtgctgga aggaagaggc tgagaggaac gagtcggtgc tgagggaggc tttggtcaca
      781 cccagcaata cctcagagaa cttggcgggc gacttgccgt cagcaagcaa ttgctcgtta
      841 attttgcgaa gctcaagccg gtccactgca tcgccgggca ggaacttggt atcgccgggg
      901 cgtgtgacct gaatcttgct catcatcttg cggatgatca cttcaaaatg cttgtcgtga
      961 atgttctgac cttgtgagcg atataccttc tgcacttcgg tcatcaaata catctggcag
     1021 gcttcgcgtc cctggatctt caacactcga tgtgggttga gcgaaccttc cgttagcggc
     1081 tggccggctt cgatcttttc attgctcttg atcagcaggc gggatgtggt tgggatctca
     1141 taagagactt cttcacgctg gtcatacgaa acgatgactt tccgttcctt cttctccacg
     1201 cgaactcggc caccatgctg ggcaacgatc tttgcgtcgt cctgggttgc cagcacatcg
     1261 ccagccttga cctctgcctc atccttgacc gcgaatttcc attcttcggg gatgctgtac
     1321 tcatcgctga cgagttcact gtgttcgatc gtgatctcgc gcaggtcagc atatttttca
     1381 gattggttga ctcggacggt tccactgatc tctgccacaa ccgcttcacc tttcggctgt
     1441 ttgcgcgctt cgaagagttc ctccacgcgg gggagaccgg ttgtaatatc tgcaccgcca
     1501 gcggcaacac caccggtgtg gaatgtgcgc aatgtcaact gcgtaccagg ctcaccgatg
     1561 gattgagcag cgacgatacc gactgcagag ccaaggtcaa ccatggcacc gcgtcccaga
     1621 tcaagaccat aacacttgga acaaatccca tgctgcaatt cgcaggtaag cgcagaacgg
     1681 accttgacct cttcaacacc agtagctgcg atcttgcgag ccagttcctg attgatgatg
     1741 tcgttgtact cgccgatgac ttcaccagtc ttcgggtcga tcacattctc tgccagcaat
     1801 cggctataga gacgactgct caaggcctga ccagcaacat catcacgcct gcggatatac
     1861 acgccatcgt gggtgccgca gtcatgttca ttgatgatga tgtcctgagc gatatccacg
     1921 aggcggcgag tcaggtaacc tgcatccgct gtacgcagcg ctgtatccgc cagacctttg
     1981 cgagcaccat gcgtcgaaat aaagtattcc tgcgctgtaa gaccttcacg gaagttcgaa
     2041 cggatcggca tcgggatgat gcgcccggat gggtcagcca taagaccgcg cattccggct
     2101 agctgcgaga tggtcgagaa accacctttc gtcgccccag aggaggccat ggtggagagg
     2161 ttgccatctg gatccatatg tttcttgaca gcatcggcca cacggtcggt tgtgccctgc
     2221 cagatttcga tctcgcgttc gttcttttcc tgctcggtca agagaccgcg gcggaagtcc
     2281 cgctggacca attccacctg tttgagggct tcgtcgataa tgccctttcg ctccggtggg
     2341 atggaaatat ctgcaacagc cagtgtcgtg ccagacctca tggcatattc aaagccaagg
     2401 gctttgatct gatctgccac ttccgtcgtc ttttcctgac cgcacacttc atagacatca
     2461 gcaatcaggt ctttcacgcc acctttgtct agtttctcat taacgaactg aacttcttcc
     2521 ggcaggatgc ggttgaaaag tacacgtccg acggttgtat caataatacg cgtttcagct
     2581 tctggcaggc gatcaccctt ttcgtcatac caggtcttgg cacgcagctt gacctcagag
     2641 tggacttcca cctggccaag cacatacgcc atttctactt cgtcgatatc cgcgaaggcg
     2701 cgtccatcgc ccttgtgatt ggctttctgc gccatggtca gatagtaaac gcccagcacc
     2761 atgtccttgg aaggcgaaat aatcggttca ccgtctgcag gcttgagcag gttcttggaa
     2821 gcaagcatta agtcacgcgc ttcctggacc gccttttgtg aaagcggcac atggacagcc
     2881 atctgatcgc cgtcgaagtc cgcgttgaac gctgtggtca ccaacggatg taattgaatg
     2941 gctgaacctt cgatcaggat cggctcaaag gcctggatac ccaggcgatg cagtgttggg
     3001 gcacgattca aaagaacagg tcgttcgcca atcacgcctt ccagcgcttc ccaaacttct
     3061 gggcgattgc gctcgatcaa gcggcgcgcg cccttcacat tcgccgcgta gttattctgc
     3121 accaggcgtg caatcacaaa cggacgatac aactcaagag ccatgctctt gggcaaacca
     3181 cattgataca gcttgagctg cggaccgacc acaattacgg agcgaccgga atagtctacg
     3241 cgtttgccca acaggttacg gcggaagcgt cccttcttgc ccttgagcat atcgctcaac
     3301 gacttcagtt cgcgacgacc acgtcgactc agcgctttgc cacgctgtga gttgtcaatg
     3361 aggctgtcaa ccgcttcctg caacatgcgt ttctcattgc ggatgatgac atccggcgcg
     3421 ccgagttcga gcaatctctt caagcgattg ttgcggttga tcacgcgacg atacagatcg
     3481 ttcaaatcgg aggtcgcgaa acgtccacca tcgagctgca ccatcgggcg caggtctggc
     3541 gggatcacag gcagaacggt gaggatcatc cactcagggc ggttgccaga gcgacggaac
     3601 gcctctacaa ccttcaagcg agtggtagcc ttcttgcgct tctgcttgga cttggttgtg
     3661 cgcacctcgt gccacagttc aacggagagt ttatcgagat cgaggcgctc gagaatatca
     3721 tagaaagctt ctgcgcccat atcggcacgg aagacctgtc cccagcgctg cttgagttca
     3781 cgataacgga tctcgctgat gaatgtgaat ggacgtaatt ccatcagttc atcgcgcatg
     3841 cgggaagact ggttggatga aacagaagtc tgatcctcaa gttgggagcg gagaccttcc
     3901 attttcattt ctgcatcagc cttgacccgc ccagcgtcca tcttgaggtc ttccagttcc
     3961 ttggcgcgtt gatccttcag ttcgttctca accgcttcga gattcttctg aacgattttc
     4021 tgaacttgag taagatgctt ggcggcgact ttatcgcccg catccaggat cttttcatca
     4081 gtcaattcaa agatgacagc cttcttggca tgctcaccca tttgaccctg aagcaacttt
     4141 tcgagcttct gaccctcttt gatgacaggg tcgagctttt cggcaatgac ttcatcgtag
     4201 ccctgctcaa ggctggcgcg cttctgattg atctcattca gcgttcgatc gcgctcggtt
     4261 ttagcttcca caattttggc attgatgccg gctgcctgct cgcgctcggt aacagagatt
     4321 tcatcctcaa tacgcttgag agccttgttg cgggcctctt catcaacata cgtgacaatg
     4381 tattgcgcaa agtagagcac acgatccagg ttgcggcgcg aaatatcaag cagcattccc
     4441 aggtaggagg ggatgcgccg cgtataccaa atgtgtgcaa caggtgtagc cagggcgata
     4501 tgacccatgc gctcacggcg gacagccgcg cgcgtcactt ccacaccgca cttatcacag
     4561 acgatgcctt tgtaacgggg gtttttgtat ttaccgcagt aacactgcca gtcacgttgc
     4621 ggtccaaaaa tggcttcaca aaataagcca tctttttcag ggcgcaagcg gcggtagttg
     4681 atcgtttccg gcttgagcac ctcaccataa gaccatgaca tgatcgtttg aggtgatgcc
     4741 aggctaatac ggagtgcagt caatcccttg gtttccacta ggtactctcc ttaataaata
     4801 attcaaaaag agcatgtctc tgcttttccc ggtgatttca gagacaaaca gaagcaaacg
     4861 cttcgagaag ttctctcaag cgcttgctgt gtttttactc agttttattt gatccaacga
     4921 tgaatattat acgcctgcga aggaaaaagt caagcccggc tccataagag ttatatctga
     4981 attcccctgt atcaggataa aaaccctctt gcaaacgaca ggacgttctg aacatcattg
     5041 aaattcgtga ccatcccaac ggaaatacgc acggcgccca tggatttgcc atcaaaacac
     5101 atgcggaagt cgtcctggga cagacgttct tcgttgcctg ggctggtaaa acaaacatcc
     5161 agttctacgc gtgagacacc catggcaact tccccagctc caggattgca gaaacagccg
     5221 gtgcgcaagg aaatattcgc ttcattggct ttttgctcaa tgagacgatg ataaatcaac
     5281 tggttgtctt tatcataaaa attcactgtc actgccccgc cgcgtccgtc tgtagtgacc
     5341 ggtccaaaga cacgcgtcaa ttggatccct gtactgtgct tcatggatgt caggttatcc
     5401 agaagccagc ccgtgagcga gtggacgcgt tcgtggatca catcatagcc aattgattca
     5461 atgtgcttca gcccaatctc aaccgccggt atattaagat aatcgatcgt tccatcctca
     5521 aaggcagagg cgccatctgc catatagtat ttatctccca tcacagaagc taccgtaatt
     5581 gtcccaccgg cgaaccaggg ccgttgcaat tgtcccaagg cctctttacg agcaattaat
     5641 gcccccagac ccgttggata accgaacatt ttataaaatg agattggaac aaagtctggc
     5701 ttgacctggc ttaaatcaag ctggcatgtc ggaacataag cagccgcgtc aagcagaaca
     5761 tcccagcctt tttgatgagc cttttcgatc cagtcgagcg ggtgctgaac cgaggaaaag
     5821 ttggattgtg caggataggc aaatagattg tgaccgtttt tcgaagtccg cgccaactca
     5881 cgctccagtt gtgactcgtc gactcgcatg tcaggcaaag caactggaat ataggtcacc
     5941 tctgcgccgc gggcatgggc aaattcgcga attccattga cggaattgtg attatcgaac
     6001 gtcaacaaat aacgcccccc gcggaacgga taggattcgc caaccagttt caaggcccca
     6061 ctggcgttgg ttgtgaagat cgccaggtat tcatcagggt ccgcattaaa aaacttcagt
     6121 acatactcac gggcgtgttc taccatttgt gtggccgcat gagaggtagg attactcgaa
     6181 tgcgggttgc caaagacatg ctctgcaagc aaccgattat gctggcgcaa ttgagaatca
     6241 gcgtataggc cgcccccggt gtaatcgaga taaatatgcc cgccaaggtc aaggcgggca
     6301 tactcgacag cgcgaagctc gtcaataatc ttagtgctcg gatacgcagg atacgccctg
     6361 agaaatgaaa caaaatcagg atcaggtgtg gtcatctggc ctccaccgtt aatttaacca
     6421 cagatatgcc gtcagatgca aacgcttata attacctcat gccccgtatt tgcatcgtac
     6481 caaaagttga tgggctggct ggagtcgcct cgtttcgtct caagtttgaa gacggacttc
//
LOCUS       DASTJP010000374         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_665891, whole genome shotgun sequence.
ACCESSION   DASTJP010000374 DASTJP010000000
VERSION     DASTJP010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665891"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1628)
                     /locus_tag="VFQ23_11050"
     CDS             complement(96..1628)
                     /locus_tag="VFQ23_11050"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET9907174.1"
                     /translation="MRVRFDTTDMNSPAQDSSSKIHTLLAKKTLILASNRGPVTLHEN
                     ERGELKYQRGSGGLVTALAGVIQHTNARWIACARTTEDRDWQQGIAPLGHGDEQVWMR
                     FLTPSEEAYEGYYRVIANPLLWFLQHSMWNIFREPTINREIWEHWENGYVGVNRLFAE
                     AIAHEITNAPHPALVMLQDYHLYLVPSLIRYQFRPTASYTLAHFVHIPWPGAEDWGFL
                     PAKMRLAILEGLCAVDLLGFQTREDALNFIRTCETHLPGSHVNYRHGKVRFRNHTTHI
                     RDFPISIDVDGLRRFAESPEVDSYRDQLLQLHGDWQIILRIDRIEPSKNIVRGFQAFD
                     ELLELHPEHRGKVKFLALLVPSRLGVNEYGSYLDEVMGVAGRINAKYGGNDWEPIRVF
                     VEENFPRAVACLQIYDVLLANSIADGMNLVAKEGPIVNCRDGVLVLSERTGAYQQLEP
                     GVLVVSPLDIYGTAEAMHQALTMSTEERQERTERLRSLIESQDIGDWLRQQLYAIMNL
                     NL"
     gene            1888..>2300
                     /locus_tag="VFQ23_11055"
     CDS             1888..>2300
                     /locus_tag="VFQ23_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9907175.1"
                     /translation="MPTTIHDVAQKLNISIYTVSRALNGYDDVADQTRQLVIETANKM
                     GYEANRAARQLRRQKTETIGFIIPSISERFDEPFFAEFISGLGAELSRKNFDLLVSNA
                     TTDDGERALYHRWTNSHKVDGFILNRIRKHDWRVGL"
BASE COUNT          605 a          574 c          528 g          593 t
ORIGIN      
        1 taaacgagcg tgagtcagaa ttacattctc tacgcctcca ataactggag gagccgaata
       61 gtgtaaaaga ccaatccttg ttaatttttt tgtcatcata gattcaaatt cataatcgcg
      121 tagagttgct gacgcagcca atcgccaata tcttgactct caattagaga gcgcaagcgt
      181 tcagttctct cttgccgttc ttcagttgac atggtcagtg cctgatgcat ggcttctgcc
      241 gtaccgtata tgtccaaagg tgaaacgacc aacaccccag gctcaagttg ttggtatgca
      301 cctgttcgtt ccgagagtac caagaccccg tcgcggcaat ttacgatagg gccttctttg
      361 gcaacgagat tcataccatc ggcaatggaa ttcgcaagca acacatcata gatttgcaga
      421 catgccacgg cgcgaggaaa attctcttct acaaatactc ggataggctc ccaatcatta
      481 ccaccatatt tcgcattaat ccggcctgcg acccccatca cttcatccaa ataactgcca
      541 tattcgttta caccgagacg ggaaggcaca agcaatgcca ggaacttaac tttgccgcgg
      601 tgctctggat gaagttcgag caactcatcg aatgcctgaa agccgcggac gatattttta
      661 ctcggctcaa tgcggtcaat gcgcagaata atctgccaat ctccgtggag ttgcagtaac
      721 tgatcacggt acgaatctac ttcgggagac tcggcaaatc gtctgagacc atctacatca
      781 atcgagatag gaaaatcgcg gatgtgggtg gtatggttac ggaaccgaac tttgccatgt
      841 ctgtagttga catgggaccc tggcaaatgg gtttcgcagg ttcgaatgaa gttcagtgca
      901 tcctcgcgcg tttggaagcc aaggagatcc actgcgcata agccctcaag gatcgccagc
      961 cgcatttttg ctggcaggaa tccccagtcc tcggcgccag gccaaggaat gtgtacaaag
     1021 tgagctaagg tatagcttgc cgttggtcgg aactgatagc gaatgaggct gggtactaaa
     1081 tagagatggt aatcctgcag catcaccagg gccggatgtg gagcgttagt gatctcatgt
     1141 gcaattgcct ctgcaaaaag cctattgacc ccaacatatc cattttccca gtgttcccag
     1201 atctctcgat tgatcgtggg ctcgcgaaat atgttccaca tggaatgctg caaaaaccat
     1261 agtaatggat tagcaattac tcgatagtaa ccttcatagg cttcctctga aggtgtaagg
     1321 aagcgcatcc agacctgttc atccccgtgc cccagaggtg ctattccttg ttgccaatct
     1381 cgatcttccg ttgtccgggc gcaggcaatc caacgagcat tggtatgctg gatcacccca
     1441 gccagagcag taaccaaccc gccacttcca cgctgatact ttaactctcc acgttcattt
     1501 tcgtgaagcg taaccggacc ccgattggaa gcgagtatta atgttttttt ggccagaagt
     1561 gtgtgaattt ttgaactcga atcttgagca ggggaattca tatctgtagt gtcaaaacgc
     1621 actctcatct ttccgtctcc tggactggaa actttccagt tccataagaa caatgtggat
     1681 ggacccagat cgtcatcata ttcgtgcatc tgtgtatcgc cattattgtt ttttggttca
     1741 tactttctcc tgggattgta gtagcccgat gtcacgcccc cacgtaactt ggccgtagat
     1801 ttcaattttg tataggattt tccctaaacg tttaggtaaa ttataccata tcgtggtaca
     1861 ataccctaaa cgtttaggag aacaaatatg ccaaccacta tacatgacgt tgcccaaaaa
     1921 cttaatatct cgatctacac tgtctcgcgc gccctgaatg ggtacgatga tgtggccgat
     1981 cagacccgtc aacttgtcat cgaaacggcg aacaaaatgg ggtatgaggc aaatcgcgcc
     2041 gcgcgacagc tgcgtcgtca aaagaccgaa acgataggtt tcatcattcc gtcgatttca
     2101 gaacgatttg atgagccgtt ctttgcagag ttcatctctg gactgggagc agaactatcc
     2161 agaaaaaact ttgacttgct ggtttcaaat gcaaccacgg acgatggaga gcgtgccctt
     2221 tatcaccgat ggacaaacag ccacaaagtc gatggattca tcctgaatcg aattcgcaaa
     2281 cacgattggc gggtcggcct
//
LOCUS       DASTJP010000375         6703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_561506, whole genome shotgun sequence.
ACCESSION   DASTJP010000375 DASTJP010000000
VERSION     DASTJP010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561506"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFQ23_11060"
     CDS             <1..353
                     /locus_tag="VFQ23_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HET9907176.1"
                     /translation="HRLNELTLDNLLSGKVSSSDFGITADGLRLQATIAERAGRPNLA
                     QNLRRGAELVEIPDDLVLKIYELLRPGRAQSVDELRASANELRENYGANETALLMEEA
                     ALAYERRGIFQRRF"
     gene            431..3133
                     /locus_tag="VFQ23_11065"
     CDS             431..3133
                     /locus_tag="VFQ23_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9907177.1"
                     /translation="MTQIYDVAIIGAGAVGSAIARELSRYELDIVLLESNSDIGMGTS
                     KASTALWHTGYDATPGSLESILLKRSYPLMEKFMVEVGSPFERLGGLLLAWTQEQLES
                     LPYLLEKAHQNGDADVYIISREELYQREPNLGEGALGGMFVPGEGILCTFTIPLACAT
                     QAVVNGVTLKLNFTVGSIKRSDDVSLISNGAEEIQAKWVINAAGLYSDQINNHFGYED
                     FKVTPRRGELIVFDKLARPLVNHILLPVPTAITKGVLISPTIYGNVLLGPTAEDLPDK
                     TATNTSASGLHSLLDKGQKILPALLNEEVTATYAGLRAATEHSDYQIALHADQKYICV
                     GGIRSTGISGSLGIAEYVADLLKEGGVALKPKAEFKTIRIPNIGEAFLRPYRNAEWIE
                     KNPSYGQIVCHCERVTLGEIHDAMYSDIPATTLDALRRRTRATQGRCQGFNCHASLVV
                     SLRGAAARKQSAVRTENPLATSLRLRSTPLSAVDVLIVGAGPAGMAAALELKKLGIDN
                     VLVAEREPEAGGIPRMCGHIGFGLTDLYRVLTGPSYARKYRDLADRAGIKIQTSTTVT
                     SWNDPSLSNGEKKYALDFTSPNGLGTIEAKAVLLATGVRERPRSARLVPGHRPQGIYT
                     TGSLQRFVYEHELPVGKRAVIIGAERVSLSVVLTLMHAGVHVLNMITELPHHQLYLPL
                     FLPAKIFFADILARAPILTNQRVTNIFGRPRVEGIEITDTASGKTRIIECDTVVFTGD
                     WIPENELARKGNVHTRRPALGPQVDSQFRTSQPGIFAAGNLLRGVETADWAALEGRGA
                     AHSIAKFLGNAKWNESRLEVHAEPPLDWVYPNVLSPDALPSRFFIRAHNFTDQGALNI
                     TQGGKVLHRQKFRRLLANTTLTLSGEWVKQVDFSGEPLKIEIAE"
     gene            complement(3203..4012)
                     /locus_tag="VFQ23_11070"
     CDS             complement(3203..4012)
                     /locus_tag="VFQ23_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9907178.1"
                     /translation="MSKVILIMSDALRYDVAKSHMGFLGHLVETKQATLYKIVGELPS
                     MSRPMYETIHTGIPTSEHGIIANTILRRSTKPNVFQSVVEAGKVTAAVAYYWVSELYN
                     RAPFDWIDDKEQDDDSSLVQHGRFHRTDDYPDEESFSSAAHLIRKYSPDYLLLHPVMM
                     DYMGETYGSDSKQYRRQAIKQDVLIGTVFTEWQERGYTILVTGDHGINSDGHHGGTAS
                     EQRKVPLYIISPDGKGKGDTGETVSHLQIAPTILKLLDVPIPSTMKMKPLS"
     gene            complement(4275..5126)
                     /locus_tag="VFQ23_11075"
     CDS             complement(4275..5126)
                     /locus_tag="VFQ23_11075"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET9907179.1"
                     /translation="MNAELVLDAKAMLGEGPAWDAKTQTLYWLDILGMRVYAGTRTLA
                     QLEELVGCLAPCKNGHLILGTRFDLVDLEPDSGQLQTLACLDSEAPTNRINDGKCDPA
                     GNFLVGTMDMNEKDPVGTLYSFNGKAVTAFFGGVTISNGLAWSTDHKTLYYIDTPSRE
                     VKAFDYDTATGKASNMRIVIRVEEALGWPDGMTSDMAGNLWIAMWGGAQVTKWNPNTG
                     ELLEQIAVPALHTSSCVFGGRDMNELYITSARTGLSNRDLIKYPLSGGLFKVMTNTTG
                     MKTFEFG"
     gene            complement(5170..6174)
                     /locus_tag="VFQ23_11080"
     CDS             complement(5170..6174)
                     /locus_tag="VFQ23_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9907180.1"
                     /translation="MEYRRLGRSGVLVSPICLGTMNFGGATNQADSFTIMQSAVDAGI
                     NFFDTANVYNNGESERITGEFLKEKGLRDQIFLATKVYNRTGNQPNDWGGTRYHIIKA
                     CEDSLRRLQTDHIDLYQLHRPPMTHSQEETLRALDDLIHAGKVRYIGCSTHPAWMVME
                     ALAISERFGLNRYISEQPPYNLLDRRIENELIPLCQKYDIALIPWSPLAMGILAGRYT
                     EATSYPQDSRAQLWDNHMVKARVNQRGIDVGTAVSKMAQERGLTSSQLALLWIKDQPG
                     ITAPIIGPRTLAQLEDATGILERKFDEADRALFDDLVHPGTAVADFHNSNDWMKARIQ
                     "
     gene            complement(6502..>6703)
                     /locus_tag="VFQ23_11085"
     CDS             complement(6502..>6703)
                     /locus_tag="VFQ23_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907181.1"
                     /translation="NDIILRAVADPTVSTPTVPPITTDVHPSVSTKSTADRLQEIDDL
                     HKLKFLTDPEYQEQRAKVIAEL"
BASE COUNT         1638 a         1698 c         1707 g         1660 t
ORIGIN      
        1 gtcatcggtt gaatgaactg actcttgaca acctcctgtc cggaaaagta tcttcctcag
       61 acttcggcat aacagccgat ggattgcggc tccaggcgac gatcgccgaa cgggctggac
      121 gaccaaacct ggctcagaac ctcaggcggg gtgccgaact ggttgagatt ccggacgatc
      181 tcgtgttgaa gatctatgaa ctgttacgac cgggccgagc tcaaagcgtg gacgagcttc
      241 gcgcttcagc aaatgaactc cgtgagaact atggagcaaa cgaaactgcc ttgctcatgg
      301 aagaggcggc cttggcgtat gaacgacgcg gtatttttca gcgtcgcttt tgagtattgc
      361 taattctttt ggcttaccct cttattgaaa atcggatcat aaataattct tcccagaata
      421 aagaaaactt atgacacaga tctacgatgt agctatcatt ggcgcaggtg cggtgggctc
      481 cgcgattgcg cgagaactct cacgttatga actggatatc gtcctccttg aatccaattc
      541 agatatcggg atgggcactt caaaagccag cactgcgctc tggcatactg gctatgacgc
      601 gacgcccggt tcccttgaat caatattatt gaaacgcagt tatcctttga tggaaaagtt
      661 tatggtggaa gtgggaagtc catttgagcg attgggtggt ctgttgcttg cgtggactca
      721 ggaacaactc gagtccttac cgtatctcct cgagaaggcg catcagaacg gggatgcgga
      781 tgtgtacatc attagcagag aagagctata tcaacgtgag ccaaatctgg gcgaaggcgc
      841 attgggaggg atgtttgttc ctggcgaagg cattctctgc acgtttacca ttcctcttgc
      901 ttgcgcgaca caggccgtgg tcaatggggt gacgctcaaa ctgaacttta ctgtcggttc
      961 gatcaaaaga tcagatgatg tttccctgat ttcgaatgga gctgaagaga ttcaagccaa
     1021 atgggtcatc aacgctgcgg gtctatactc cgatcaaatc aataaccatt ttgggtatga
     1081 agacttcaag gtcacgccac gccgcgggga attgatcgtg ttcgacaagc tggcgcgtcc
     1141 gcttgtcaat cacattcttc tgccggtgcc gacggcaatc acaaagggtg tgctaatcag
     1201 cccgaccatt tatggcaatg ttctgctcgg gcccactgcc gaagacttgc ctgataaaac
     1261 ggctaccaat accagcgcca gcggattaca ttctctgctt gacaaaggtc aaaagattct
     1321 gcctgcactg ctcaacgaag aagtgacagc gacgtatgcg ggtctacgcg ccgccacgga
     1381 gcatagcgat tatcagatcg ctcttcatgc cgatcagaaa tatatttgcg tgggtggaat
     1441 tcgttcaaca ggcatctcgg gaagcctggg cattgcagaa tatgttgcag acttgctgaa
     1501 agaaggcggc gtcgcgttaa aacccaaagc tgaattcaaa acgattcgca tcccgaatat
     1561 cggggaagca tttctccgac cctatcggaa tgcagagtgg attgaaaaga atcccagcta
     1621 tggacaaatc gtttgtcact gtgagcgcgt aacgctgggg gaaatacacg atgccatgta
     1681 ttctgatatc ccagcgacta cgctggatgc cttgcgtaga agaacgcgcg ccacccaggg
     1741 acgatgccag ggatttaatt gccatgcttc tctagttgtg tcattgcgag gagcggcagc
     1801 gagaaagcaa tctgctgtca ggacggaaaa tcctttagca acttcgcttc gacttcgctc
     1861 cactccgctc agcgcggtag atgtcctcat cgtcggagcc ggaccagcag gcatggccgc
     1921 agcattggaa ttaaagaagc tagggattga caacgtcctg gttgcagagc gtgagccaga
     1981 agccggcggc atcccgcgca tgtgtgggca tatcggcttt ggtctcacgg atctataccg
     2041 agtgctgaca ggtccctcct atgcacgcaa atatcgggat ctggcagatc gggctgggat
     2101 aaaaattcag acgtcaacga cagttactag ttggaatgat ccctctctct ccaatggaga
     2161 gaagaaatat gctctggatt tcacttctcc aaatgggctc ggaaccattg aagcgaaagc
     2221 tgttttgctt gcaaccggcg tgcgcgagag accgcgttcc gcgcggctgg ttccggggca
     2281 tcgcccgcag ggaatataca ccactggctc actgcagcgc tttgtctatg aacatgaatt
     2341 gcctgtaggg aaacgcgcgg tgattatcgg tgccgagaga gtgagtcttt cggttgtgtt
     2401 gactctcatg cacgctggag tgcacgttct gaacatgatt acggaactgc ctcatcacca
     2461 actttatctc cctttgttct tacccgccaa aatattcttt gccgatatcc tggcgcgtgc
     2521 accgatcctc accaatcaac gcgtaacgaa tatttttggc cgtccgcgcg tggaagggat
     2581 cgaaataaca gatacggcct ctggcaaaac tcggatcatc gaatgtgaca ccgttgtttt
     2641 cactggcgat tggatccctg aaaacgaact agcccgaaaa ggaaatgtac acacgcgcag
     2701 acctgcgctc ggcccccagg tcgattcgca atttcgcacc tcacagccag gcatcttcgc
     2761 cgccgggaat ttactccgtg gtgtcgagac ggcggactgg gcagctttgg aagggcgcgg
     2821 cgcggcccac tccatagcaa agtttttggg gaatgccaaa tggaacgaga gcaggctaga
     2881 ggtccatgcc gaaccgccac tcgattgggt atatccgaac gtgctcagcc cagacgcatt
     2941 gcccagtcgc ttcttcattc gtgctcataa tttcacggat caaggtgcct tgaacattac
     3001 gcagggagga aaagtgttgc atcgtcagaa attcagacga ctacttgcaa atacaacact
     3061 aactctttca ggtgagtggg tgaaacaggt tgatttttct ggcgagcctt tgaagatcga
     3121 gattgccgag tgatgtatta agcaaaacaa aagccttgtg aatgatcttc acaaggcttt
     3181 ttcatttctc gttttctgcg tgctatgaca gaggtttcat cttcatcgta gaaggaatag
     3241 gaacatcgag cagctttaaa atagtcggcg cgatttgcag gtgagagaca gtctctcccg
     3301 tatcgccttt gccctttcca tccggtgaga tgatatataa cggcaccttg cgctgctcag
     3361 atgcagtccc accgtgatgc ccatcgctgt tgatgccatg atcgcccgtg acgaggatcg
     3421 tgtagccacg ttcctgccat tctgtaaaaa cggtaccgat gagaacatcc tgtttgatcg
     3481 cctgcctgcg atactgcttt gaatcggagc cgtaggtttc gcccatgtag tccatcataa
     3541 cggggtggag cagcaaataa tcaggcgaat attttcttat gaggtgcgcg gctgatgaaa
     3601 acgattcttc gtctggataa tcatcggttc ggtgaaaacg accatgctgg accagtgacg
     3661 aatcatcatc ctgttcctta tcgtcgatcc aatcaaacgg cgcgcggtta tacaattcag
     3721 acacccaata atacgccacc gcggccgtga ctttgcccgc ttccaccaca ctttgaaaga
     3781 cgttgggctt cgtcgagcgc cttaggatag tattggcaat gatcccatgc tcactggtgg
     3841 gaatcccggt gtgaatagtt tcgtacatag ggcgtgacat actgggcaat tccccaacga
     3901 tcttatacag cgttgcctgc ttagtttcca cgagatgccc taaaaatccc atatgggact
     3961 tcgcgacgtc atagcgcagg gcatcagaca tgatcaggat tactttgctc attgttgtgt
     4021 tacatgtcct ttcaaaaaga atactttcct gagaattgag caattatatc tcagtgccag
     4081 gcggcactta atgtatagct ttgctggtgg caatagagat aaaatgacta tctgaggttg
     4141 atctcaatta gggaatcagt attgaagtaa aatctggggt tatggcagaa cgccccgacg
     4201 ggattcaaac gtttggacag tgggtcaagc accgaaacac aaccgtgcct gatggattcg
     4261 ggctcacggt aatcctaccc aaactcaaag gtcttcatcc ccgtcgtgtt cgtcatgact
     4321 ttgaacaacc ccccagaaag cggatatttt attaaatccc tgttgctcaa gcccgttcgt
     4381 gcagaggtaa tgtaaagctc gttcatgtct cttccaccaa acacacagga agaagtatgg
     4441 agcgcaggca ccgcgatctg ttctaataat tcccccgtgt tcggattcca ctttgttacc
     4501 tgagcaccac cccacatggc gatccatagg ttcccagcca tatcggatgt cattccatcg
     4561 ggccagccca aagcttcctc cacacgaatc acgatccgca tattggacgc ttttccagtt
     4621 gccgtatcat aatcaaacgc cttgacttca cgcgaagggg tatcaatgta atacagcgtt
     4681 ttgtgatcgg tactccaggc gagcccattc gaaatggtga ccccgccaaa aaatgccgtg
     4741 accgctttcc cgttgaagga atatagcgtt ccaaccgggt ctttttcatt catatccatc
     4801 gtgccgacaa ggaagttccc agcggggtcg catttgccat cgttgatgcg attggtaggc
     4861 gcttcagaat ccagacaggc gagagtttga agctgaccag aatccggttc caggtctaca
     4921 agatcgaagc gtgtcccgag gatgagatgt ccgtttttgc agggggccag gcagccgact
     4981 aactcctcca gttgggcaag agtccgcgtg cccgcgtaga cgcgcatgcc aagaatgtcg
     5041 agccagtata aggtttgtgt ctttgcatcc cacgccggac cttcacccag catggccttt
     5101 gcgtcgagga ctaattccgc gttcatcacg ataaccaatt ccgatctaaa gcagatgccg
     5161 tgtgtgcctt cactggatgc gcgccttcat ccaatcattg gaattatgga agtcagcaac
     5221 tgcggtgccg ggatgcacaa ggtcgtcaaa caaagcacgg tcagcctcat caaatttcct
     5281 ctccagaatt ccagtggcat cttccaactg cgcgagggta cgcggcccga taatgggcgc
     5341 ggtgatccct ggctgatctt tgatccacaa gagagccagt tgcgaagagg ttaatccacg
     5401 ttcctgcgcc attttgctta cagctgttcc aacatcgata ccgcgctggt tgacacgcgc
     5461 cttgaccata tgattgtccc aaagctgtgc gcgcgaatcc tgcggatagc tcgtggcctc
     5521 ggtgtaccgt ccggccagaa tgcccattgc cagtggtgac caggggatga gcgcgatatc
     5581 gtatttttga cataagggaa tgagttcatt ttcgatgcgg cggtccagta aattataagg
     5641 aggctgttcg ctgatataac ggttgagccc aaagcgctca ctgatcgcca gcgcttccat
     5701 caccatccag gcgggatgtg tggaacaacc aatatagcgg acctttccgg catggatcag
     5761 atcgtccagc gcccgaaggg tttcctcctg agaatgtgtc atgggcggcc gatgcagttg
     5821 gtaaaggtca atgtgatctg tttgcaagcg tcgcagcgaa tcttcacaag ccttgatgat
     5881 atgataacgc gtcccgcccc agtcattggg ctggtttccc gtgcggttgt aaacttttgt
     5941 agcaagaaag atctggtcac gtagcccttt ttctttcagg aactcgccag tgatccgctc
     6001 gctctctcca ttgttataga cattcgccgt gtcaaagaaa tttatgccgg catccacagc
     6061 gctttgcatg atagtaaaag agtctgcctg gtttgttgct ccgccgaagt tcatcgtccc
     6121 taagcagatg ggagaaacca atacgccaga acggcccagt cttctgtatt ccatgtctat
     6181 cacctcacta ctttgaaatt tttacagaag acattatagc ggactcgcat gatactttat
     6241 cccttcaatg agctgaccaa ccagcaacag acatgccaaa gtgtaaagct tcttgttcat
     6301 tgaggctaga tttggcgttc cccctatcca aaacacaaac cagtcagcat ctcctactcg
     6361 gaagtctgac tggcctcagc aactttaatt gccccgacgc ggacgtgttc caaactacct
     6421 tccacacata aggaggtttg atcgtgtacg gtttggcgga aacgctcagg attgagtcag
     6481 ctatggtgac tcaatcctgg ttcatagctc ggcgataact ttggcgcgtt gctcctggta
     6541 ttccgggtca gttaggaact tcagtttgtg caaatcatca atttcttgaa gtcgatctgc
     6601 agtgcttttt gtggaaacag atgggtgaac gtcggttgta ataggcggaa ctgtaggcgt
     6661 agaaacagta ggatcggcaa cagcccgtag aatgatgtca ttt
//
LOCUS       DASTJP010000376         4241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_805728, whole genome shotgun sequence.
ACCESSION   DASTJP010000376 DASTJP010000000
VERSION     DASTJP010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805728"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFQ23_11090"
     CDS             <1..507
                     /locus_tag="VFQ23_11090"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9907182.1"
                     /translation="VVSQFTLTAAAFTPTSSIPTNTSAPAVTAPPAATATATGPVLAQ
                     VTNASGTPEDLCDSMTYDVATVDVNVPDNTVMSPGQDFIKTWKVRNNGSCEWGAGYVL
                     AYSGYTDQMSGQFVALTDVVLPGEDVEVSVLFTAPSQAGTYLSAWQMRNPAGVTFEEI
                     IFVKIIVQ"
     gene            513..1616
                     /gene="queG"
                     /locus_tag="VFQ23_11095"
     CDS             513..1616
                     /gene="queG"
                     /locus_tag="VFQ23_11095"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET9907183.1"
                     /translation="MSQELKQSIKEKARLLGFTLAGVTTPEAPPHYATFENWLAREHH
                     GTMNYLATDKARARRANPREILPECKSILVLATPYSPPLPASSSLPLRSARVGEGRGE
                     VRGRVASYAWGEDYHDVLPARMKELVQFIEAQVGGPVKNRYYTDTGPILERDLAQRAG
                     IGWIGKNTCLINPKQGSYFLLSEIFLDLELEPESPFVTDHCGTCTRCITACPTQCILP
                     DRTLDARRCISYLTIELKEDIPAELRSLMGDWVFGCDVCQMVCPWNRFAVEGDPVFGD
                     DPPSSIRATQALTDELTLTPQEFNQRFKGTPLKRTKRRGYVRNVAVALGNMGDMHALP
                     VLNQALTDPEPLVREHAKWAIQEIHKKEISSRK"
     gene            1971..2741
                     /locus_tag="VFQ23_11100"
     CDS             1971..2741
                     /locus_tag="VFQ23_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9907184.1"
                     /translation="MKAEKRSKRQERRDRMQQRERRNRLVTIGIITLVAAFLVFAIVW
                     PQIRPIAEVVSVETSPRSNVDRNAVGDPNAPIKIEEFADFQCPFCERFFEQTEPLLMQ
                     HYVDTGKVQFVYRSMGNFVSQNMGGARTESQDAAAAAYCAADQGKFWEMHDALYFNVL
                     GEDAGSFTDRRLGAIAESVGLDMNEFNDCYSSGKYADQVQQDFADGQAAGVTGTPGFV
                     VTYTVNGETKTTMIAGAQPFSEFQVQLEAILNEIEAAS"
     gene            3208..>4241
                     /locus_tag="VFQ23_11105"
     CDS             3208..>4241
                     /locus_tag="VFQ23_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET9907185.1"
                     /translation="MKRRLVLSLNCFLLFALILTSCAGGPGLAAFQSPTPTVVTAAPT
                     TVPQAFPLALVETDPPPNSLIGHLSPITFYFNQAMNRTSTESALSGLPAGTFAWNDDS
                     TLLFTPNQPYAPDTTLNLSISNTVQSASGFGIAEPIELAFPVSDYLRTTNILPKADAE
                     DVVVDAAIAVSFNQPVVALGGDPASQPAAFNIQPAVVGRGEWINTSTFVFYPEPAMAG
                     GTEYTVSLNQDLKTVTGVGLPETGGEGNSWSFTTSRPRVVALEPVSDQLLDIEPEIKL
                     TFNQPMDKASVESNFVFSGTEGTVNGSFTWNDDATELIFAPENPLARDTGYILNVGAA
                     ARSQNGEITL"
BASE COUNT         1009 a         1179 c         1097 g          956 t
ORIGIN      
        1 gtggtctcgc agttcacact tacggctgcg gcgttcacac ccacttcatc catccccacc
       61 aacacctcag ctcccgcggt gactgcccct cctgccgcga cggccaccgc cacaggaccc
      121 gtccttgcac aggtgacgaa cgcctccggc acgcccgagg atttgtgcga tagcatgacc
      181 tatgacgtcg ccacagtgga cgtgaacgtc cctgataaca cggtgatgtc gccagggcag
      241 gatttcatca agacctggaa ggtcagaaat aacggttcct gtgaatgggg cgcgggatac
      301 gtccttgcct attcaggcta taccgatcaa atgtctggtc agtttgtggc acttaccgat
      361 gtggttctgc cgggagagga cgtggaagta tccgttctat ttacagcccc atcgcaggcg
      421 ggcacctacc tcagcgcctg gcagatgcgc aacccggccg gcgtcacctt cgaggagatc
      481 atcttcgtga agatcatcgt gcagtaagat ttgtgtctca agaattaaaa caaagtatca
      541 aagagaaagc acgcctgctt ggcttcaccc tggcaggcgt gacgactccg gaagcacctc
      601 cccattacgc gacattcgaa aactggctgg cacgcgagca tcatggcacg atgaattatc
      661 ttgccacaga caaagctcgc gcccgccgcg cgaatccacg cgaaatcctg cctgaatgca
      721 aatccatcct tgtgctggct acaccgtata gtcctcctct ccccgcttcg tcttcgctac
      781 cgctccgctc agcgcgagtg ggagagggtc ggggcgaggt cagagggcgg gtagcctcct
      841 acgcctgggg cgaagattat cacgatgtac tccccgcgcg gatgaaagaa ctcgttcaat
      901 ttatcgaggc gcaggtgggc ggtccggtga agaaccgcta ttacaccgat accggtccga
      961 tcctcgaacg cgacctcgcc cagcgtgcag gcatcggctg gatcgggaaa aacacctgcc
     1021 tgatcaatcc aaagcagggt tcatactttt tgctctcaga aatttttctt gatctcgagc
     1081 tcgaacctga atcccccttt gtaaccgatc attgcggcac atgcacccgc tgtattaccg
     1141 cctgccccac acaatgcatc ctgcctgatc gcacgcttga tgcacgtcgc tgcatttcgt
     1201 atctaacgat cgagctcaaa gaagatattc ccgctgagtt gcgttctctg atgggcgatt
     1261 gggtcttcgg gtgtgacgtc tgccagatgg tctgcccgtg gaatcgattc gcggtcgaag
     1321 gtgacccggt atttggggat gaccctccgt cttcgatcag ggcgactcag gctctgaccg
     1381 atgagctgac tctgactccg caggaattta atcagcgctt caaagggact ccgctcaaac
     1441 gcaccaagcg acgcggctat gtgcggaacg tggcggtggc attgggcaac atgggagata
     1501 tgcacgcgct acccgttctc aaccaggcgc tgacagaccc ggaaccattg gtgcgcgaac
     1561 atgcaaaatg ggccatacag gaaatccaca agaaggaaat ttcttcccgg aaatgaatag
     1621 ttcttcttat gatttttgtc taaaccctga cagtttgtct caaaaacgat ccgttggtcg
     1681 agtgccgaag gcgtacagtt gcatgattta ccaagcaaat cacacgcaag tgtacgcccc
     1741 tccgcttaca ttcgggacta ctcgaccgac ggaactcgcg agaaatctaa aactgcaggt
     1801 aactcgattt tcattaaacc tcccttactt actactagac aaaacaatat gggcatggga
     1861 aaatcggcgt cggattaaga gtagtaacga ctttgcgaag catccctttg ggatcagtcg
     1921 tttagcgact aaagtcgcta ctacaagtat ttttttatca aggagttttc atgaaagccg
     1981 aaaagagaag taagagacag gaacgacggg atagaatgca gcagcgggag aggcgaaatc
     2041 gcctggtgac catcggaatt attacacttg ttgcagcatt tctggttttt gcaatcgtct
     2101 ggccccagat cagacccatt gctgaagttg tctcagtaga aaccagccct cgatcgaatg
     2161 tagatcgcaa tgcggttggt gacccgaacg cgcccatcaa aattgaggaa tttgctgatt
     2221 tccaatgtcc attctgtgaa cggttctttg aacagaccga acccttgctc atgcagcatt
     2281 atgtcgatac aggcaaggtt caatttgtct atcgctcgat gggtaacttt gtcagccaga
     2341 atatgggcgg cgccaggact gaatcacagg atgccgcggc agccgcttat tgtgcagcag
     2401 accagggcaa gttctgggaa atgcatgatg ctttgtattt caacgtgctg ggtgaggatg
     2461 ccggttcatt caccgaccga cgacttggtg ccattgccga aagtgtaggg ctggacatga
     2521 acgaattcaa cgattgctac agcagcggta aatatgcaga tcaggtccag caggattttg
     2581 cagatggcca ggccgcgggt gtgaccggta cgccaggttt cgtcgttacg tatacggtta
     2641 acggtgaaac aaaaacaacg atgatcgccg gtgctcagcc attcagcgaa ttccaggtgc
     2701 aactggaagc gatcctgaac gaaatcgaag cagcgtccta gtcaacacag caaaaggaca
     2761 tggattgttc catgtccttt ttgattctgc gaatgtcatt gcggtctctg ttggtcgagt
     2821 agccctgtcg cgctgagcga agtcgaagcg tcggggcgta cacctgcact gcagcgaacg
     2881 cagtgcggtg caagtgtcga gaccaaaggc gagcaatctc ctcattaagg atgacgctgc
     2941 acggcttcgg gctaatgccc tcgcaattac acaatcccgg agtgtaggtg gatcactgag
     3001 ttcatcgacc ccgcatgatt tgcttggcaa atcacgttcg gggcaggtgg aaaaaactca
     3061 aaagactctg tgggctctgt ggctaagccc aagaaattag acactcccca caatcaacaa
     3121 gtgtataatc atttggaatc ccattttgat tacgccatcc gcagtgaccg gaaaactgtt
     3181 tcgagcagat tttattgagg tccccgaatg aaacgtcgcc tcgttctctc cctgaattgt
     3241 ttcctcctat ttgcattgat ccttacctca tgcgccggcg gtcccggtct ggcagcattt
     3301 caatcgccta cccccactgt cgtcactgca gcaccgacaa cggttccaca ggcgttccca
     3361 ctggcactgg tggaaacgga cccacccccc aacagcctca tcgggcacct ctctccgatc
     3421 acattttatt tcaaccaggc aatgaaccgg acctccacag aatctgcctt gagcgggttg
     3481 cctgccggca cctttgcgtg gaacgatgac tcgactcttt tgtttacacc caaccagccc
     3541 tatgcgcctg atacaacact gaatctgagc atctcaaaca cggttcaatc agccagcggg
     3601 tttggaattg ccgagccgat cgagcttgcg tttcccgttt cagattattt gcgcaccacg
     3661 aacatcctgc caaaggcaga tgcggaagac gtggtcgttg atgccgccat cgcggtttcc
     3721 tttaaccagc cggtcgtcgc ccttggaggt gacccggcct ctcaacccgc cgctttcaac
     3781 attcagcctg ctgtggtcgg gcgtggcgag tggatcaata caagtacctt cgtcttttat
     3841 ccggaacccg ctatggcagg cgggacggaa tacaccgtca gcctgaacca ggacttgaaa
     3901 accgtgacag gggtcggctt gcccgaaacc ggaggcgaag ggaactcctg gtcgttcaca
     3961 acctcccgcc cgcgcgtggt cgcgttggaa ccagtcagcg accaattgct cgacattgaa
     4021 cccgaaatca aactgacctt caaccagccc atggataaag ccagtgttga atccaacttt
     4081 gtgttcagtg gaacagaagg cacggtgaac ggctccttca cctggaacga cgatgcaaca
     4141 gagttgatat ttgccccgga gaatccgctc gctcgcgaca ctggttatat cctgaatgtg
     4201 ggtgcagcgg cccgttcgca aaatggggag atcacccttg a
//
LOCUS       DASTJP010000377         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_973016, whole genome shotgun sequence.
ACCESSION   DASTJP010000377 DASTJP010000000
VERSION     DASTJP010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973016"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFQ23_11110"
     CDS             <1..790
                     /locus_tag="VFQ23_11110"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET9907186.1"
                     /translation="LPNGQVLREYNITALNKNIEVVPGIEYPAWTYNGRIPGPTLRAT
                     EGDRIRINFSNGSNHPHTMHFHGFHPGNMDGVPGSGPGGNVLPGGSFVYEFDAEPFGL
                     HLYHCHAFPLARHIAKGLYGAFIVDPKETRPPVDHEYIMVMSGFDVDFDDANDFYAVN
                     AIPFHYQNHPIQIKVGESVRVYLVNILEFDLINSFHLHANFFQYYPTGTSLTPAEFTD
                     TIIQGQAQRGILEFTYKYPGMYMFHAHVTEFAELGWNGMFEVTE"
     gene            800..1987
                     /locus_tag="VFQ23_11115"
     CDS             800..1987
                     /locus_tag="VFQ23_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907187.1"
                     /translation="MTTSTTPTSRFSLRTLILLLIPIILLAGVIWLFLATGGGLNLEA
                     PVPVEDLNIERYTLTRDVIELHVRNTGPDELTIAQLAVNEAIMPFTVTPSTTIPRLGQ
                     AVVHIPYPWSDGEAYGVTVFTTNAIAFAVDIPVAFITPQPTNETFWGFTLIGLYVGVI
                     PIFLGIFWFPALRSLGRRTMTFLMAVTAGLLIFLGLDTLAEALEFAAAVPGPFQGIGL
                     IGIGAVATFLLLDAITKQQTALTGSEADRRLAVAFMIAVGIGFHNLGEGLAIGAAYNV
                     GEIALGTFLVVGFIIQNITEGLGIIAPVLRDRPSLGRLALMGLVGGAPAILGAWIGGY
                     TPSPFLAVLFLAIGTGAIFEVVYEIAKLIQKDTQREAMPMTVFSGVLVGMLLLWVTGL
                     LIK"
BASE COUNT          522 a          592 c          540 g          593 t
ORIGIN      
        1 cctgccaaat ggacaggtct tgcgggaata caacatcact gcacttaata agaacatcga
       61 ggtcgttccg ggaatcgaat atccagcatg gacttataac ggtcgaattc ctggtccgac
      121 gctgcgcgca accgaaggtg accgcattcg catcaacttc agcaacggaa gcaatcatcc
      181 gcatacgatg cacttccatg gctttcaccc cggcaatatg gacggtgttc cgggttcagg
      241 tccgggaggg aatgttttac caggcggatc attcgtgtac gagtttgacg ctgagccatt
      301 cggtttacat ctgtatcact gccatgcttt tcctctcgca cgtcatatcg caaaaggact
      361 ctatggggca tttatcgttg atccaaagga aactcgcccg ccggttgacc atgaatacat
      421 catggtcatg agcggctttg acgtggactt cgatgatgcg aacgattttt acgcagtcaa
      481 tgccattcct tttcattatc aaaatcatcc tattcaaatc aaggtcggcg aatcggttcg
      541 cgtgtatctc gtcaatatcc tggaatttga tttgatcaat tcattccacc ttcatgcaaa
      601 ctttttccag tactacccca ccggaaccag cctgacacca gctgaattca ccgatacgat
      661 cattcagggg caggcccagc gcggcattct tgaattcacc tacaaatatc ctggcatgta
      721 catgttccat gcacacgtga cagagtttgc tgaactcggc tggaacggca tgttcgaagt
      781 gacggaatag agacctatca tgactacctc aaccactccg acctcacgct tcagcctcag
      841 gactcttatc ctgcttctta tcccgattat tctcctcgct ggggtgatct ggcttttcct
      901 cgctaccggc ggaggcttga atctggaagc acctgtgcct gtagaagacc tgaatattga
      961 acgttacaca ctcaccagag atgtaattga attacacgtc cgcaataccg ggccagacga
     1021 actcacgatt gcgcagctcg cagtaaatga agccattatg ccatttactg ttacaccaag
     1081 cacaacaatt ccgcgtctgg gtcaagctgt ggtccatatt ccgtatccgt ggtcagatgg
     1141 tgaagcgtat ggcgtcaccg tttttaccac gaatgctatt gcgtttgctg tggatatccc
     1201 tgttgcattt attacgcctc agccaacgaa cgagactttc tggggcttta cccttattgg
     1261 tttatatgta ggtgtaattc ccatttttct cggtattttc tggttccctg cgcttcgctc
     1321 actcgggcgc cgcacaatga cgtttctcat ggcggtaacc gcaggcttat tgatctttct
     1381 tggcctggac actctggccg aggcactgga gtttgccgcg gccgttcctg gtccatttca
     1441 ggggattggc ttgatcggca tcggagcagt tgcaacattc ctattgttgg atgccattac
     1501 gaaacagcag accgcgctca ccggcagcga ggctgaccga agactcgcgg tcgcttttat
     1561 gatcgcagta gggattggtt tccataacct tggcgagggt ctggcgattg gtgcagccta
     1621 taacgttggt gagattgctc tgggcacctt ccttgtggtg ggcttcatta ttcaaaacat
     1681 cacggaaggt ctcggcatca tcgccccggt cctacgcgac cgaccgagtc tgggcagact
     1741 cgcactcatg ggtctagtcg gcggcgcacc agcgatactc ggtgcctgga tcggtggata
     1801 taccccatcg ccatttttgg cagttctctt tcttgcgatt ggaaccggag caatcttcga
     1861 ggtggtttat gagatcgcca aactcattca aaaagataca caacgcgaag caatgcctat
     1921 gaccgtcttt agtggcgtgc tagtcggaat gttattgctc tgggtcacag ggttgcttat
     1981 taaataacgt cactgcgagg agctgcaaag cagcgacgtg gcagtctcaa ctaatctaca
     2041 aaaaagcggt taattgagca acgagattgc cacggcgggc aagcaccgcc tcgcaacgac
     2101 gtctgtaacg gaaaaataaa catggaaaac atcattactc cccttcgaat cacgggctta
     2161 ctcgctttgc ttggtgcctt cttatatgca atcggcgatg tgcttttact ggccagcaaa
     2221 gtcaatctcg atgactatcc aaagctc
//
LOCUS       DASTJP010000378         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1074913, whole genome shotgun sequence.
ACCESSION   DASTJP010000378 DASTJP010000000
VERSION     DASTJP010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074913"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..665
                     /locus_tag="VFQ23_11120"
     CDS             63..665
                     /locus_tag="VFQ23_11120"
                     /inference="COORDINATES: protein motif:HMM:NF025757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HET9907188.1"
                     /translation="MKTNNQFQGRHWETGSIHNALALQGVKAPHTGKPYSEALLLGIS
                     GGITFGYFTFEYKGYLPHVAMLMRNTFSPFSTTVERLGIAQDIQQTNKAEIAEKNLRN
                     ALDSGLFPLLWADQFSLPYNGLPSNEPMWGMMPILAVESDGKSVAIADRSSQPFHLSM
                     AELTKARGRVKDDKFRLITLDAPQTAKLAGAVHKGILPGD"
     gene            604..1272
                     /locus_tag="VFQ23_11125"
     CDS             604..1272
                     /locus_tag="VFQ23_11125"
                     /inference="COORDINATES: protein motif:HMM:NF027495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HET9907189.1"
                     /translation="MHLRLPNWPVRSIRAFCQAIKLFTEEPPRGGRDNFGFAAYEKLA
                     HLLVNTRNKQSWERFFAPGVRMYHALAGSPVQPGAYHWVMTWGSADGAERGLYADFLD
                     EAAQILKKPGLKESAEKFRESYQLWLAFADALLPDNIPLLGESKKLIQKKQDLFINKG
                     ESALADVQKINSRLNELLEVSKTDFPLSDAQAAEFRSNLRNILLKISAVEQQAIDFLQ
                     NAIL"
     gene            complement(1317..1730)
                     /locus_tag="VFQ23_11130"
     CDS             complement(1317..1730)
                     /locus_tag="VFQ23_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907190.1"
                     /translation="MKRWQGSCIGALLVLLIGGGSLFFFVPVGGAMLVGIPPVFDRLT
                     GWVVCPGALSISQQEYNSGPVTTSPSGGTGHQEEWTCTFEDGSQKVVPNEEIALKGIG
                     ASFTAAGICGGLVVLLLIIVAAAIGARLVKPRDDL"
     gene            complement(1965..>2172)
                     /locus_tag="VFQ23_11135"
     CDS             complement(1965..>2172)
                     /locus_tag="VFQ23_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907191.1"
                     /translation="NTHVTDGTFDCAYNLPAGGGVAIVNANSHETLTEILSAYPLQPW
                     VEYEIHSLSDLKHSFDLTIKALGG"
BASE COUNT          579 a          527 c          538 g          528 t
ORIGIN      
        1 cttcaagttt acttacacac gtcagacatg caatagatag gcggccaaat ttggagatac
       61 atatgaaaac aaacaatcaa tttcaaggtc ggcactggga aacgggcagc atccataatg
      121 cgctggcttt gcagggtgta aaagccccac acaccggcaa gccttattca gaggcattat
      181 tgcttggcat cagcggagga atcacatttg gttattttac ttttgaatac aagggatact
      241 tgccgcatgt ggctatgctc atgcgcaaca cgttcagtcc tttttcgacc acagtcgaac
      301 gattaggtat cgcacaggat atccagcaga ccaacaaagc cgaaattgca gagaaaaatt
      361 tgcgaaatgc gctggacagt ggattgttcc cgttgttgtg ggcagatcag ttcagtcttc
      421 cttataacgg acttccttct aatgaaccga tgtggggcat gatgccaatc cttgccgttg
      481 aatcagatgg aaaatctgtg gctattgcag accgatccag ccagccgttt catttatcta
      541 tggcagagtt gacaaaagcg cgcgggcgcg tcaaggacga taaatttcgg ctcattaccc
      601 tcgatgcacc tcagactgcc aaactggccg gtgcggtcca taagggcatt ttgccaggcg
      661 attaagttat tcacagaaga accgccgcgc ggtgggcgcg ataacttcgg cttcgcggcg
      721 tatgaaaagc tggctcattt attagtcaac acacgcaaca aacagagctg ggaaagattc
      781 tttgcgccag gtgtgcggat gtatcatgcg ctggcaggtt ctccagtgca gcccggagcg
      841 tatcactggg tgatgacctg gggatccgca gacggagcgg aacgcggtct gtatgcggat
      901 ttcctcgatg aagccgctca gatcctcaag aagccaggtt tgaaagaatc ggctgagaag
      961 tttcgcgaaa gttatcagct atggcttgcg tttgcggatg cattgctgcc ggacaatatc
     1021 cctctgttag gcgagagtaa gaagttaatt cagaaaaagc aggatttgtt catcaacaaa
     1081 ggcgagagcg cactggcaga tgtccaaaaa ataaattcgc gattgaatga gctgttggaa
     1141 gtatctaaaa ctgatttccc gctttcagat gcacaagcag ctgagttccg gtcaaattta
     1201 cgcaatatcc ttttgaagat cagcgcggtt gagcagcagg ctatcgattt tttacagaac
     1261 gcgattttat aatctgaagc cagagttcgg aagttttgaa aacttccgaa ctctttttat
     1321 aaatcatccc tcggcttgac caaccgcgcc ccaattgctg ctgcaacgat gatcagtaat
     1381 aacacaacca acccaccaca aattccagcc gcggtgaacg aggcgccaat acctttgagc
     1441 gcgatctctt catttggcac tactttctgg ctgccgtctt caaaggtaca agtccattct
     1501 tcctggtgac cggtcccgcc agatgggcta gtcgttacgg gccctgaatt gtattcttgt
     1561 tgagaaattg aaagcgcgcc cgggcaaacg acccagcctg ttaatctgtc gaacacgggt
     1621 ggaattccga cgagcattgc gccacccacc gggacaaaga aaaacaacga accgcctccg
     1681 atcaataaaa caagcagcgc accaatacag gatccctgcc agcgtttcat attctctcct
     1741 tgaacaagag atatttatag agagattata gcaatagaaa gagaaacgtg cagagcctgg
     1801 cattgatcaa gaagcatggt actcgcgtga aaatttcctc ccacgcgatt cattacgttt
     1861 gggagggatt ggggtcgtcc ctcccgtatt attcgataca cttgcctgcc gcagcctgag
     1921 tcccgctcag atgcaccaaa gatgactgtc ctaaaccctg aggtctatcc acctagagcc
     1981 ttgatggtca ggtcgaaact gtgtttgagg tcagacagag aatgtatttc atattccacc
     2041 catggttgaa gcgggtaggc gctcagaatt tctgttagtg tttcatgtga attcgcattg
     2101 acgattgcca caccgcctcc ggccgggagg ttgtaggcac agtcgaacgt accatccgtc
     2161 acatgggtgt tg
//
LOCUS       DASTJP010000379         4085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_982821, whole genome shotgun sequence.
ACCESSION   DASTJP010000379 DASTJP010000000
VERSION     DASTJP010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982821"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="VFQ23_11140"
     CDS             complement(<1..1944)
                     /locus_tag="VFQ23_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9907192.1"
                     /translation="MRSANNPSLPTGTVTFLFTDIEGSTKLAQQYPDAMPALLARHHE
                     ILRQVIQAQNGYIFQNEGDSLAVAFHSAMDALIAASCAQQLLQSEAWTPAPIKVRMGI
                     HTGTAQLNEPSAPTIYTGYTTLAMASRVMSCGHGGQVLLSRATHALVHNSLPADTELI
                     GLGEKRLKDLLQPEHLYQLNTAGLQMSFPALKTMDVSLTNLPLQLSTFIGREKEVQQI
                     KKRLEKNRLVTLTGSGGVGKTRLSIRVAEELLNQYPNGVWLVELAPVTDPDLVTQTIC
                     AALDVTPQGNTPALTVLTNYLKQKKILLVVDNCEHLIDSCAQLCEALLHTCPNLQIIA
                     SSREALGIDGENAYRVPSLSLPNLKDGLSTIEQSEAVKLFVERAAATLPEFKITKANA
                     AAIAQICQRLDGIALAIELAASRVKLLKVEQIASRLDDAFRLLTGGSRTALPRQQTLR
                     ALIDWSYNLLSEEEKTALRRLSVFVGGWTLEAAEHVCDNSNILDLLTHLVDKSLVAID
                     LEHGDEPRYYFLETIRQYTREKLFDSNEMKATRQRHLTYFLAFAEMKGTETQGRDQIT
                     ALQKLDDEYENIHEAMDWAIETGQMEEATQITVALAWLYWWSRGLTQEAYEKINLILD
                     HPATTKEKLFRAVALILAALYAPA"
     gene            complement(2077..>4085)
                     /locus_tag="VFQ23_11145"
     CDS             complement(2077..>4085)
                     /locus_tag="VFQ23_11145"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9907193.1"
                     /translation="IAGTAETVVNGIPLRVWAMRGKEHLGTFAMEYTARLLPWYEEYF
                     GAPYYYDKYDQVAVPGFAAGAMENSGLVLFRQELLIMEPGNVSWRQEKSIAHVIAHEF
                     AHMWFGNLVTMQWWDDLWLNEAFAEWMSYRVISELSPEYRLWDDFGGDKNYTLGLDAL
                     ETTHPIYSPVETPAQAEELFDAITYIKGCAVMRMLENFLGTGSFRDGLRTYMQEFATR
                     NARGADLWRHLQAASQQPVTQIMESWILQGGHPCVTALLETDDKNQLQLSQKRFFENP
                     QAPKDNRQLWLVPLVIRYEDDSGTHVLRHVLAEASETLTLPVNGDLHWCYLNADQIGF
                     YRQNLSSTLFDNILTHVGQLTASEQMGLLGDQWALVRSGLQPISQFLDVLTAMSSQSD
                     NYNLLSEIVEHLQTLEKMVEDLEDASILDQFRSWVGGLFKERLNALGFEPQTDEPIEV
                     SQQRVSVLRAMTALAHDQAAIAQVRRLAEREAEDPNSVDPNLAASIVAINAKFGDDRL
                     FRRHVDIYQARKANGAPPQTVTRYLYSFAYFREPELVTQTLNLFDEGIAPTEGLMLVL
                     NQMLYARHSQLEAWQYIKDNWSTIKEIGLGSSELIKSAGRLPYRMRSDFVAFCEAYVK
                     GVEDLSYAQALETMDLLAEFQARTKDDLITWLNNVSMRSQRQAG"
BASE COUNT          974 a         1034 c         1039 g         1038 t
ORIGIN      
        1 ggctggagca tacaaagcag caagaatgag tgctacggcc cggaatagct tttctttggt
       61 cgtcgcggga tgatcaagaa taagatttat cttctcatat gcttcctgag ttaatccacg
      121 actccaccaa taaagccagg caagggcaac agttatttga gtcgcttctt ccatctgacc
      181 tgtctcaatt gcccagtcca ttgcctcgtg aatattttcg tattcatcgt caagcttttg
      241 tagagcagta atttgatctc tgccttgtgt ttcggtgcct ttcatttcgg cgaaagcaag
      301 gaagtatgtc aaatggcgct gtcgggtggc tttcatttca ttcgaatcga acagtttctc
      361 acgcgtgtat tgacgaatcg tttcaagaaa ataatagcgc ggctcatcgc cgtgttcgag
      421 atcaatcgcg accagggatt tatccacaag gtgagtgagc agatccaata tattcgagtt
      481 atcacaaaca tgctctgcgg cttcaagagt ccaaccgcca acaaaaacgg ataaccgccg
      541 caaggcggtc ttttcttcct ctgatagaag gttgtaactc cagtcaatca aggcgcgtaa
      601 ggtttgttgg cgtggcaggg cagtacggct tccaccggta agcagccgaa aggcatcgtc
      661 gaggcgggat gcgatttgtt ccactttcaa tagtttcacg cgcgaagcag ccaattcaat
      721 cgccagggca atgccatcca gccgctgaca gatttgggcg atagcggctg cgttcgcttt
      781 tgttattttg aattcgggca aggtggcggc ggcccgttcc acgaataatt tcaccgcttc
      841 cgattgttca atggtggata gtccgtcctt tagattcggc agcgagagcg aagggacgcg
      901 ataggcattt tcgccatcaa tccccaacgc ttcacgactg ctggcgatga tctgcagatt
      961 gggacaagtg tgtaagaggg cttcacaaag ctgcgcacaa ctgtcaatca gatgctcaca
     1021 gttatccacc accaataata ttttcttctg tttcaaataa tttgtgagga cggtcaatgc
     1081 gggagtgttg ccctgtggag tcacatccaa agcggcgcat atggtttggg ttaccaaatc
     1141 aggatctgtg actggtgcaa gttcaaccag ccaaactccg ttaggatatt ggtttagtaa
     1201 ttcttctgcg acccggatgg agaggcgagt cttccccacg ccacctgaac ccgtcagcgt
     1261 gacgaggcga ttcttttcca gccgcttctt gatctgctgc acttcctttt cacgtccgat
     1321 aaaggtgctg agttgcagtg gcaaattcgt tagtgataca tccatggttt tgagcgctgg
     1381 aaagctcatc tgcaaacccg cagtgttcaa ttgatataaa tgttcgggtt gtagtaagtc
     1441 tttcaaacgt ttctcaccca gaccgatcag ctcggtatcc gcagggagtg aattgtggac
     1501 caaggcgtgg gttgcccttg aaagcaaaac ctgcccgcca tgtccgcagg acatgacccg
     1561 cgaagccata gccagcgtgg tatagccggt atagatggtc ggcgcggatg gttcattcaa
     1621 ttgagcggtg ccagtatgta tccccatgcg cactttgatc ggggcgggag tccatgcttc
     1681 actctgaaga agttgttgtg cacatgatgc ggcaatcaat gcatccatcg cggagtgaaa
     1741 tgctacggcc agtgaatcgc cttcgttctg aaagatatat ccgttttgag cttgaatgac
     1801 ttgtctcaaa atctcatgat gtcgcgccag cagagcaggc atagcatcgg gatattgctg
     1861 agccagtttg gtgctgcctt cgatatcggt gaagaggaag gtgacagtgc cggttggtaa
     1921 tgaggggttg ttggctgatc gcattttgta taaataatag cccgtcacgg caggctatac
     1981 aagtagtcgg atcagaagac caggtcaccg aggacagttg ggtttgacca gaacatcaac
     2041 gcaactccgt ccaattttga tgttaacgag tacgttctac cctgcctgcc tttgcgagcg
     2101 catactgacg ttgttcagcc acgttattaa atcgtctttt gtccgcgctt gaaattcagc
     2161 cagcaggtcc atggtctcca atgcctgggc atagcttaaa tcttctacgc ccttcacata
     2221 agcctcacag aaagcgacga aatcgctccg catcctgtaa gggagccttc cagcagattt
     2281 gatcagctca ctactgccga ggccgatctc tttgatggtc gaccagttgt ctttgatgta
     2341 ttgccaggct tccaattgag aatgacgggc ataaagcatt tggttcagga caagcattaa
     2401 tccctcggtc ggtgcaatcc cttcatcaaa tagattcagg gtttgcgtca ccagctcagg
     2461 ttcccggaag taggcaaagc tatagagata gcgagtgacg gtttgtggag gggcgccgtt
     2521 cgctttacgc gcctgataaa tgtcgacgtg ccttctgaac aaccggtcat caccaaattt
     2581 ggcgttgatg gcaacaatgg aagccgccag gtttgggtct acgctattcg ggtcttcggc
     2641 ttcgcgctcc gccaatctgc gcacctgcgc gatggcagcc tggtcatgcg cgagcgcagt
     2701 cattgctctg agtaccgaaa cacgttgctg tgaaacctcg atgggctcat ctgtttgggg
     2761 ttcgaagcca agagcgttta gtcgttcctt gaacaggccc ccgacccagg atcggaactg
     2821 atcaaggatt gacgcatcct caagatcttc gaccattttt tccagtgttt gcaggtgttc
     2881 aacgatttca gacagcagat tgtaattgtc actttgggaa gacatagccg ttaacacatc
     2941 caggaactgc gagataggct gcagcccact gcgcaccaaa gcccactgat ccccaagtag
     3001 tcccatttgt tcgctggcag tcagttgacc gacgtgagtc agaatgttat cgaataacgt
     3061 acttgagaga ttttgccggt agaaaccaat ctggtcagcg ttgagataac accagtgcag
     3121 atccccattt accggaaggg ttaatgtttc agaggcctcc gccagcacgt ggcggaggac
     3181 atgcgttcct gaatcatcct catagcggat gaccaggggg accagccaaa gttgacgatt
     3241 atctttcggg gcttgtggat tctcaaagaa gcgcttctga ctcaattgaa gctggttctt
     3301 gtcatccgtc tcaagcaagg ctgtcacaca tggatggccg ccctgtaaga tccagctttc
     3361 catgatttgg gttacaggtt gttgggaagc tgcctgtaga tgccgccata ggtcagcgcc
     3421 gcgcgcgtta cgtgtggcaa attcctgcat atacgtcctc aatccatcgc gaaacgaccc
     3481 ggttcccagg aagttctcca gcatgcgcat gacagcacaa cctttgatgt aggtaattgc
     3541 gtcaaacagc tcctccgcct gggcgggcgt ttccaccgga ctgtaaatag gatgtgtagt
     3601 ttcgagagcg tcaagcccga gagtgtaatt tttgtctcct ccaaaatcat cccagagacg
     3661 gtattcaggc gaaagctccg aaatgacccg atacgacatc cactcggcaa acgcctcgtt
     3721 gagccacaag tcgtcccacc attgcatggt cacgagattg ccaaaccaca tgtgcgcaaa
     3781 ttcatgtgcg atcacgtgcg caatggattt ttcctgtctc catgaaacat tccccggttc
     3841 catgatcagg agttcctgtc gaaacaatac cagaccagag ttttccatcg caccggctgc
     3901 aaatcccggt acagcgacct gatcatattt atcgtaataa taaggagcgc caaagtattc
     3961 ttcataccag ggcaagagtc gggcggtata ttccattgca aacgtgccca ggtgctcctt
     4021 gccgcgcatt gcccagaccc gcaacggaat gccattgacc accgtttcag ccgtcccggc
     4081 gatat
//
LOCUS       DASTJP010000380         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_740469, whole genome shotgun sequence.
ACCESSION   DASTJP010000380 DASTJP010000000
VERSION     DASTJP010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740469"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /gene="cydB"
                     /locus_tag="VFQ23_11150"
     CDS             complement(<1..239)
                     /gene="cydB"
                     /locus_tag="VFQ23_11150"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET9907194.1"
                     /translation="MSYEFLQTLWFILIAVLWIGFFFLEGFDFGVGMLLPFLGKKDIE
                     RRAIINTIGSVWDGNEVWLLTAGGATFAAFPHWYAT"
     gene            complement(255..1658)
                     /locus_tag="VFQ23_11155"
     CDS             complement(255..1658)
                     /locus_tag="VFQ23_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET9907195.1"
                     /translation="MDPLILSRWQFGLTTIYHFLFVPLTLGLGWFVAYMQTRYYQTRD
                     EDYRKMAKFWGKLFLINFAIGVVTGIVQEFQFGMNWSEYSRYVGDIFGAPLAIEALLA
                     FFLESTFLGIWIFGEGKVPEKVHLASIWIVAMASNFSALWILLANGWMQHPVGYVINE
                     INGRAELVDFFALITNPKGWLFFWHTIAAGFATASFVILGVSAYHIARGQNLRIFKES
                     FQMAALVGLIAGVLVFLGGHTNGQYMYETQPMKFAAIEAHWETSDPASFSILTIGDLT
                     GKREVWSIRVPRVLSLLACNNLTCEVRGVDDIQKEYESLYGAGDYIPYIVFTYWSFRS
                     MMTVGFIMIALSAFFLFMTRGSYENAKWMKWVPWVIALPYIANTSGWILTEMGRQPWI
                     VQGLLKVEDAVSPNLTTVDLLISLIGYTVVYGSLGVAMFYLMKKFAIAGPDATLHESV
                     DVEEDSLSEALPIGAQG"
     gene            1801..>2609
                     /gene="lgt"
                     /locus_tag="VFQ23_11160"
     CDS             1801..>2609
                     /gene="lgt"
                     /locus_tag="VFQ23_11160"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9907196.1"
                     /translation="MTAIQFPEVYMIDPVIFTIRLFGMEFPLRWYGVIVMAGVIVGSM
                     IVEREIRRRGENGERIWDALLWVLPIGIIGARLWFVLNATLGGNRFYIENPASIIRIW
                     EGGLHIFGGFLFGAAALLWYLRQNKLDPWLFLDAAGPALLIGQGIGRLANFINQELYG
                     PPTTLPWGIPISAEHRLPEFQDLSLFPVETTRFHPTFAYEMLWNFTTAGLLLWLSRRY
                     EKELKPGAIFAGWLIAAGLGRVIIEFFRPDQPKIPDLGISYTSIISALMAIA"
BASE COUNT          696 a          660 c          609 g          644 t
ORIGIN      
        1 gtggcatacc agtgcggaaa tgcagcaaag gttgctccgc cagcggttaa taaccagact
       61 tcattgccat cccacaccga tccaatcgtg ttgatgatag cgcggcgttc gatatccttt
      121 ttgccgagga aaggcaggag cattcctacg ccaaagtcaa aaccttccag gaagaagaag
      181 ccaatccaca atacggcgat cagaataaac caaagggttt gtaaaaattc gtacgacatg
      241 atttgtctcc ttacttagcc ctgggcacca attggcagtg cttcagacaa actatcttct
      301 tctacatcaa cggactcgtg caaggtggca tctggtccgg caatggcgaa ttttttcatc
      361 agatagaaca tggctactcc caatgacccg taaacaacag tgtaaccaat cagggaaatc
      421 aacaaatcta ccgtggtgag atttggagag accgcgtctt caaccttcaa caaaccttgt
      481 acaatccagg gttgccgtcc catttcggtc aaaatccagc cgctcgtgtt ggcgatatac
      541 ggtagcgcga tgacccacgg tacccatttc atccatttgg cgttttcata ggatccgcgt
      601 gtcatgaata agaagaaggc cgagagcgcg atcatgatga accccaccgt catcatggag
      661 cggaaggacc aataggtgaa aacgatgtaa ggaatataat ctccggctcc gtagagagat
      721 tcatattcct tctgaatgtc atccacaccg cgaacttcac aggtcaggtt attacaagcc
      781 agcagactta gtacccttgg aacacgaatt gaccacactt ctcgctttcc cgttaggtcg
      841 ccgatcgtca gaatggagaa agaggcaggg tctgaggtct cccagtgtgc ttcgatggcg
      901 gcaaacttca ttggttgagt ctcatacatg tattgaccat tagtgtgtcc gcccaaaaac
      961 accagaacac cggcgatcaa gccgaccaga gctgccatct gaaaggattc tttaaagatc
     1021 ctcaggtttt gcccgcgagc gatgtgatag gcactcacgc ccagaatcac aaaggaagcg
     1081 gtggcaaagc cagccgcaat cgtatgccag aagaacaacc agcctttggg attggtgatc
     1141 aatgcaaaga aatccactaa ctcggcccgt ccattgattt cattgattac atatccaacc
     1201 ggatgctgca tccaaccgtt cgcaagcagg atccatagtg cggaaaaatt cgaggccatt
     1261 gccacaatcc aaatggaagc caggtgaacc ttttcgggca cttttccctc tccaaagatc
     1321 catatcccca gaaaagtcga ctccaggaaa aatgccagta acgcctcaat ggcaagtggc
     1381 gcgccgaaga tatcacccac atatcgtgag tattccgacc agttcatgcc aaactgaaat
     1441 tcctgaacaa tccccgtaac tacaccgatg gcaaaattga tgagaaataa ctttccccag
     1501 aacttcgcca ttttgcggta atcttcatcc cgcgtttgat aatagcgggt ttgcatgtac
     1561 gccacaaacc accctaggcc caatgtaagc ggaacgaaaa gaaaatgata gatcgtggtt
     1621 agtccaaact gccatcttga aagtataagt ggatccataa agtctccttc tggctatatt
     1681 gtgaattata tcacaagcta ctgctcggtc aaggtttatg acctgacact ctcaatttgc
     1741 gacatgaatc attttagttc tcgatgagtt tatgatttgt aactgtacaa aacgattata
     1801 atgaccgcaa tacagttccc cgaggtatac atgattgacc ctgtaatatt tacgattcgg
     1861 cttttcggca tggaatttcc tttacgctgg tacggcgtga tcgttatggc tggtgtcatc
     1921 gtaggttcga tgatcgttga gcgtgagatc agacgccgcg gcgaaaacgg cgagcgaatc
     1981 tgggatgctt tgttatgggt cttgcccatc ggtatcattg gcgcacgcct gtggtttgta
     2041 ttgaatgcaa cgctgggtgg gaatcgcttc tatattgaaa accccgcaag cattattagg
     2101 atatgggaag gcggtttaca tatctttggc ggattcttgt tcggagcagc cgccctcctt
     2161 tggtatctgc gccagaacaa actcgacccc tggctcttcc tggatgcggc ggggcccgcg
     2221 ctgctgatcg gtcagggtat cgggcggctt gcgaatttca tcaatcagga gctatatggc
     2281 cccccaacca cactgccgtg gggaattccc atttcggccg aacatcgtct tccagaattc
     2341 caggacttga gcttgttccc cgttgagaca acccgcttcc accctacatt tgcttatgaa
     2401 atgttatgga atttcactac agcgggcttg ttgctatggt tatcgcgccg ctacgaaaaa
     2461 gagttaaagc caggagcaat cttcgcaggc tggctgattg cagcgggact tggacgtgtg
     2521 atcatcgagt tcttccgccc ggatcagccg aaaatccctg atcttggaat cagctatacg
     2581 agtattattt cagccctcat ggcaattgc
//
LOCUS       DASTJP010000381         5418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_125078, whole genome shotgun sequence.
ACCESSION   DASTJP010000381 DASTJP010000000
VERSION     DASTJP010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125078"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VFQ23_11165"
     CDS             <1..821
                     /locus_tag="VFQ23_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907197.1"
                     /translation="TKEHIGRIIYTCTRGDFNQLCSVNADGTGFQQMTEVEAHNYYPV
                     YSPLGGSIVYSSNQNGGVFDLFLFIFEGQRLIRLTEAIGNVVSPSFSPDGTTILFANR
                     AAEGPTSLWTIENTGENSKLLYAGPNTIVAADWSPDGNTIAFAMAVDQPDAYEIFLMG
                     ADGSNVRQLTYGLAGIGGSLDWSPDGKYLLIYAGPQGDKDIFRIDVEAKTAVQLTDGG
                     NNAASSFSPDGQWIAFNSLRNNDQADIFIMRPDGSSLRQVTTDPEPDWQPQWEP"
     gene            918..1601
                     /locus_tag="VFQ23_11170"
     CDS             918..1601
                     /locus_tag="VFQ23_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907198.1"
                     /translation="MKRFVHIAVLLSLLSGCSSLPISIPFLQSPTPTSSPAPTATPFS
                     LPASNTPDLFVINTSEPTSTASNGLPLSTNTPLPIFSPTIRSTITLEPITPNLFTPSP
                     NIFLSVQKSTTQLVWGSTCDGARSIRFTAQVMPVRRLRFVTLWYRLQDKYSGRNTEWG
                     GGAILSDNDQGTYFYDLDLSQITGYRFYEDAWFQYQLVASTITRRVLGRSVVSRSEIS
                     LTHCTVFNP"
     gene            1625..2326
                     /locus_tag="VFQ23_11175"
     CDS             1625..2326
                     /locus_tag="VFQ23_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907199.1"
                     /translation="MKKTVHILLLSIFLSGCTALQNINLGQLIASPTAPPPADTETPF
                     VSATPLPTQNLFATSTSTPLTFTPTVTAIGAELFTPTNTETPAPTAGLPLDAFSGNYF
                     TPKNEGFLAVLISNNLMYWNEGPCSPRSVKVSAFVADAVNTDKVLLFTRLREKRNTLN
                     VTEWNSGAIMIKADNGSFNYDVHTFNLRRYFYFREAWLEYQLVALTEDLEVIGRTGIY
                     DRNISLVRCLPVPVP"
     gene            2323..3060
                     /locus_tag="VFQ23_11180"
     CDS             2323..3060
                     /locus_tag="VFQ23_11180"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET9907200.1"
                     /translation="MTFPAELISLLRESSHLVALTGAGVSQESGLRTFRDAQTGLWAQ
                     YKPEDLASPQAFRRDPKLIWDWYAWRREAVKGVRPNPGHYALVELETNLPKFTLITQN
                     VDGLHRMAGSQNVLELHGNIQRVRCSECYTFAETWGDDTESVPQCSVCNGLLRPDVVW
                     FGEALPRDQLEAAVEAARSCDGFFSIGTSGVVQPAASLAHAARNRGAVVVEINAEPTP
                     LTSKANYFFQGKSGEILPELVQSAWGY"
     gene            3100..3468
                     /locus_tag="VFQ23_11185"
     CDS             3100..3468
                     /locus_tag="VFQ23_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907201.1"
                     /translation="MLNGETKLKTGLIVVSLAVLLAVLNVASLKGLDLKRRSSWWYGV
                     ISFICGISVIMVLNGNLNDGQIAGIICAFLTMLGGATTRWHKQEFEGKFRSLVIEYGK
                     EDDPSLFAKLVRKLFGKYKN"
     gene            complement(3675..4556)
                     /locus_tag="VFQ23_11190"
     CDS             complement(3675..4556)
                     /locus_tag="VFQ23_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907202.1"
                     /translation="MNGLNLISCLTIIVSLALVGLVILAIKPNNSRVAPNTSAKVPGR
                     TRHGWQSWFAFISLALVISIWTIPGALFFLIIVCLVRLPARKSSSFLVSDKEKISARR
                     IYTWLLFSPMLTIPVFVVLLIGLSYGASNTDTRVLAALIPWVFHVPLLAGLTSKNAFI
                     FRHTQQGILLLAIRAGIAMIASSIGASSNTGFWIFLFGNGSLWLFGSMWGSSQVNRGE
                     CWWMEQNRENVASSEPITTVKHQMDHELEDLLKSLNTDDKVAAKSRALQTFRVGAPET
                     RKRAVEVLSKLGEVEMF"
     gene            complement(4562..>5418)
                     /locus_tag="VFQ23_11195"
     CDS             complement(4562..>5418)
                     /locus_tag="VFQ23_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907203.1"
                     /translation="SLVYATVFFGILAVGSLLTYIQVPFGNVIAFFSGIGFGLYLLAR
                     VLEQISSRVRPLSVWVTPLTYSSIFLTAAAVIVNLPFVAAYMTANAVSLAFAGALFIT
                     IAYREQQFPLGYLGVALLEVAWGLLLYTNEIRHPQLYAIPGGLYFLGIAYLEMQRNRR
                     GYALAIELLGLGVLLITSFAQSLNEQTGLMYFVLLMIESLLVVWWGVLQKRKVPFFTG
                     IGASAINIAAQVIILISVHDIHRVNRWLVAFGAGILITAIAVIAELKREQLRARSRQL
                     SEMLESWE"
BASE COUNT         1400 a         1372 c         1317 g         1329 t
ORIGIN      
        1 cgaccaaaga acatatcggg cgtattatct atacatgcac gcgcggtgac ttcaatcaac
       61 tctgctcggt caatgccgat ggcacgggct tccagcaaat gacggaggtg gaagcgcata
      121 attattatcc tgtctattcg ccgctcggtg gttcgatcgt ttattcatcg aatcaaaatg
      181 gtggcgtatt cgatctcttc ctgttcatat tcgaaggaca gcgcctgatc cgcctcactg
      241 aggcgattgg taatgtcgtc tcaccgagct tttcgccgga tggaaccacc atcctctttg
      301 ccaaccgtgc cgcggaagga ccgacatcct tatggacgat tgaaaacacg ggcgagaatt
      361 cgaaactgct ttacgcaggt ccgaatacga tcgtcgctgc agattggtca cctgatggga
      421 acaccattgc ctttgccatg gcagtggatc aacccgatgc ttacgaaatc tttctcatgg
      481 gagcagatgg ctcgaatgta cgacaattga catatggtct ggcaggaatc ggtggtagcc
      541 ttgattggtc gccagacggc aaatacctgt tgatctatgc aggtccgcag ggtgataagg
      601 atatattccg catcgatgtg gaagcaaaaa ccgcagtgca gcttaccgac ggaggcaata
      661 atgccgcttc ctcgttctcg ccagatggtc agtggattgc ctttaactct ttgcgaaata
      721 acgatcaggc tgatatattt atcatgcgcc ccgatggttc cagtctgcgc caggtgacta
      781 ccgatcccga acccgattgg cagccacaat gggaacccta agatcacttt cacttcgcga
      841 agcgaccgga atggggattg tggttgcagc acaaagtatg gcagaatata ttatgtaaag
      901 tgaagtacag aggaaccatg aaaagatttg tccacattgc agttctactc tctctgcttt
      961 caggctgttc ctccctgccc atttccatcc ctttccttca atcgccgacg ccgaccagtt
     1021 caccggcgcc cactgcgact ccattttctc tgccagcttc caatacaccc gacctgtttg
     1081 tgattaacac ctcggaaccc acctccacgg cttcaaacgg tctaccccta agcacgaaca
     1141 cgccgttgcc catcttttca ccgaccatcc gttccactat cacgctggaa cccattacac
     1201 ccaatctttt caccccttcg ccgaacattt ttctttcggt tcaaaaaagc acaacacaac
     1261 tggtctgggg cagcacgtgt gatggcgcgc gttcaatccg ctttacggct caggtgatgc
     1321 cagtgcgcag attgagattt gtgacgttgt ggtatcgcct gcaggataaa tacagcgggc
     1381 gtaacaccga atggggaggt ggcgcgatcc tgagcgataa cgatcaggga acgtattttt
     1441 atgatctcga cctgagccag atcaccggtt atcgattcta tgaagacgcc tggttccagt
     1501 atcaacttgt tgcatctacg atcacgcgca gagtgctcgg tcgatctgtc gtatctcgaa
     1561 gtgagatttc ccttacacac tgcacggtat tcaatccttg aatttgagtc gcaaagggtc
     1621 taccatgaaa aaaacagttc acatcttgtt gttatccatt ttcctttcgg gttgcaccgc
     1681 tttgcagaac attaaccttg ggcagctgat cgcctctccc acggcgccgc cgcctgcaga
     1741 tacagagact ccgtttgtga gcgcaacacc gcttcccacg caaaacctct tcgccacctc
     1801 caccagcaca cctctcacat ttacccccac ggtcacggcg attggcgctg aactgttcac
     1861 gcccaccaat acggaaactc ctgctcccac ggccggccta ccgctggatg catttagtgg
     1921 caattacttt acgccaaaga atgaaggttt tctggccgta ttgatttcga ataatctgat
     1981 gtactggaat gaaggtccat gttcaccgcg cagcgtcaag gtctcggcct ttgttgcaga
     2041 tgccgtcaat acagacaagg tcctcttgtt tactcgattg cgtgaaaaac ggaacacact
     2101 taatgtcact gaatggaact cgggcgccat tatgatcaag gcagataatg gatcattcaa
     2161 ctacgatgtc cacaccttca acctccggcg ttatttttac ttcagagaag cctggctgga
     2221 atatcaactg gttgccctga cggaagacct ggaggtcatc ggtcgtacag gcatttatga
     2281 tcggaatatc tcactcgtgc gctgcctgcc tgttcccgtc ccgtgacgtt tccggctgaa
     2341 ctcatctcgt tactgcgcga aagctcccat ctggtcgcct taaccggagc tggagtctcg
     2401 caggaaagcg gattgcgcac cttccgcgac gctcaaaccg gactttgggc gcagtacaaa
     2461 ccagaagact tagcctcgcc tcaagccttt cgccgtgacc caaagttgat ctgggattgg
     2521 tacgcctggc gacgtgaagc tgtcaagggt gtgcggccta accctggaca ttacgcgctg
     2581 gttgagctcg aaacaaacct tcccaaattt accctcatta ctcagaatgt agatggcttg
     2641 catcgcatgg cagggagtca aaatgtgctg gagctacacg gaaatattca acgcgtccgt
     2701 tgttctgaat gctatacgtt tgcagaaaca tggggtgatg atacagaatc tgttcctcaa
     2761 tgttctgttt gcaatggatt actccgcccg gatgtcgtct ggtttgggga ggcattgccg
     2821 cgcgaccagc ttgaagctgc cgtcgaagcc gcgcggagtt gtgatggttt cttttcgatt
     2881 ggcacatcgg gtgttgttca accggcggcg tctttggccc acgccgctcg taatcgaggc
     2941 gcggtggttg tagagatcaa tgccgaaccg acaccgctta cctcaaaggc gaattacttc
     3001 tttcaaggaa agtcaggtga gatattgccg gagttggtac agtcagcatg gggctattag
     3061 acattccttc tttaacgagt ggatctttat agaatattga tgctcaatgg agaaacgaaa
     3121 ttgaaaacag gtttaattgt agttagtctc gcggtacttc tggctgtact aaatgttgca
     3181 tctcttaaag gtcttgattt gaaaagaagg tcaagttggt ggtatggcgt aataagcttt
     3241 atttgcggca tttcagtaat tatggtgtta aatggaaatc tgaatgatgg acaaattgca
     3301 gggataattt gtgcgttcct gactatgctt ggcggtgcga caacgcgctg gcataagcag
     3361 gaatttgaag gtaagttcag atctttggtg atagagtatg gtaaagaaga cgatccatct
     3421 ttatttgcaa aactggtcag aaagcttttt ggtaaatata agaactgagt tgtattcacc
     3481 gtaaacggct aaacaatgca gtttatttat atttgcaaag gcaacgcatt gtattgtcgg
     3541 ggtaagacga gcgaagaaga tccactagat tggaatgctg atcgctcaaa ttgataagac
     3601 acgttttcat taccgataat tttgtgatgg attcccaacc aacatcaaca atgccatcgg
     3661 aatataattc cacactagaa catctccact tctcccaatt tagacaagac ttcaacggct
     3721 cgttttctcg tttcaggtgc accgactcga aacgtctgaa gggccctcga tttggcagcc
     3781 accttgtcat ctgtgttcag cgacttgagc aagtcttcca actcgtgatc catttgatgt
     3841 ttgacggttg ttatcggttc agaagaagcg acattctcgc ggttttgttc catccaccag
     3901 cattcgcccc ggttgacctg gctcgacccc cacatgctgc cgaaaagcca cagtgaaccg
     3961 ttgccgaaca ggaatatcca aaacccagta ttcgatgaag ctccgatact gcttgcaatc
     4021 atggctatcc ctgcccggat tgcaagaagc aggattcctt gttgggtatg gcgaaaaatg
     4081 aacgcgtttt tcgaggtcaa cccggcaaga aggggtacat gaaaaaccca gggaatgagc
     4141 gcggcaagaa cgcgagtatc cgtattggat gcaccataac tcaagccgat cagcaatact
     4201 acaaaaacgg ggatggtaag catcggtgag aaaagcaacc aggtatagat tcgtctggca
     4261 gatatctttt ccttatcact caccaagaaa gatgaactct ttcgggcggg tagtcgcaca
     4321 agacaaacaa tgatcagaaa aaagagcgcg ccagggatcg tccagatgga aatcaccagc
     4381 gcgagactaa taaaggcaaa ccaactttgc catccatgcc tggttcgtcc tggcacctta
     4441 gccgatgtgt tcggcgcgac tctcgaattg ttcggtttga tggcaagaat caccaaacct
     4501 acgagcgcca gagacacgat tatcgtcaga caggaaatga gattaaggcc attcatgtgt
     4561 gtcactccca gctttcgagc atttcgctca actgtcgtga acgcgcccgt aattgttctc
     4621 gcttgagctc ggcgataacc gcaatggccg taatgaggat gcctgctcca aaggccacca
     4681 accagcggtt gactcgatgg atgtcatgaa cggaaatcaa aatgatgact tgcgctgcga
     4741 tattgatggc gctggccccg atgccggtaa agaacggcac tttccttttt tgcaggacgc
     4801 cccaccaaac caccaataat gactcaatca tcaagagtac gaaatacatc agcccggttt
     4861 gttcgttcag ggattgggca aaggatgtga tcaatagcac gccgagtccc aacagctcga
     4921 ttgccagggc gtatcctctt cgattgcgtt gcatctcaag ataggcaatc cctaaaaagt
     4981 agagtccgcc gggaatggca taaagctgtg gatgacgaat ctcattcgta tacaaaagga
     5041 gtccccaggc aacttcaagc aatgccacgc cgagatagcc cagaggaaat tgttgttcgc
     5101 gataagcgat cgtgataaat aatgcacctg cgaaggcaag cgaaactgcg ttcgcagtca
     5161 tataggcagc tacaaatggg agattaacga tcaccgccgc ggcggtcagg aaaatagagg
     5221 agtaggttag gggagtgacc caaacactca gcggcctgac gcgagatgag atctgttcca
     5281 aaacgcgggc taatagatag agcccgaagc caattccgct gaagaaagcg atcacatttc
     5341 cgaacggaac ctgaatatac gtcagtaaag aacccacggc cagtatccca aagaacacag
     5401 tggcataaac cagcgacg
//
LOCUS       DASTJP010000382         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1131630, whole genome shotgun sequence.
ACCESSION   DASTJP010000382 DASTJP010000000
VERSION     DASTJP010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131630"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFQ23_11200"
     CDS             complement(<1..893)
                     /locus_tag="VFQ23_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907204.1"
                     /translation="MYPIQRNYQSEEDYWRIRELLRQVSLYNNRHDFSWSLLRWDYWR
                     WHINENIFHFNLQDVITIWEVNAQIVAVLNPDRDGEAFFQVHPEFQDRVFMSEMLEVA
                     EEKLSRINGRDKKELIVWVNATDTSTKSLFGEKGYARSEFSAEHMRHRFISQSTASLG
                     GIHDSISKMGYTVRALGDERELPARSWLSWKVFHPDEPDEKYESWEWYYNVQRVPLYR
                     RDLDIVAVAEDGELAAFCTVWFDDVTRTAVFEPVGTHPNHQKRGLGKAVMSEGLRRAQ
                     RLGATLATVSSYSTAAHALYES"
     gene            complement(954..1766)
                     /locus_tag="VFQ23_11205"
     CDS             complement(954..1766)
                     /locus_tag="VFQ23_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9907205.1"
                     /translation="MTDEKNPCLSGTGLTKIFGFGQNKTVAVDHVDFDFHEGEIVSIV
                     GESGSGKTTLAKMILGLINATEGEIYFQGKKLNLNTYRKKQEYWKNIQAIFQDPFSSY
                     NIFNKIDSVLLDCINMRGGRKLSHEKKVMLMTDACSFVNLKFEELTNKYPFELSGGQM
                     QRLMIARVFLLKPKILLADEPTSMIDASCRATILGMLMKLHMETGMTLIFITHDVGLA
                     YYVSDKVYIMEHGKFIESGTPDDVILHPKEAYTQRLISDVPKMHEVWDLSTV"
     gene            complement(1759..2781)
                     /locus_tag="VFQ23_11210"
     CDS             complement(1759..2781)
                     /locus_tag="VFQ23_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907206.1"
                     /translation="MSKVILDVKELTTKYITRYQEDVYAVDHVSLKVEEGKSLGIAGE
                     SGCGKSTLALSLMGYYFPPLYYTSGEINIDGRNISGMDPDNVRKSILGSEIAYIPQSA
                     MNALNPTRKIINFIEDVIHAHDEYVPRKEIYELARGSFELLGLSADVLQKYPVELSGG
                     MKQRTVIAVSVILYPKVLIADEPSSALDVTSQKMVIKMLRDLMEKGLIKSLIFITHEL
                     PLLKNVTDDIMVMYAGQIVEKGTAGETVFDPIHPYARGLMGSIIVPEKGLRECELAAI
                     PGVPPNLKNPPAGCRFAERCKYARPECKVLSIPLREADGGRAFRCLVSENELREIYAR
                     EACKND"
     gene            complement(2791..>3835)
                     /locus_tag="VFQ23_11215"
     CDS             complement(2791..>3835)
                     /locus_tag="VFQ23_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9907207.1"
                     /translation="LDDAAANRIASKLSSEDRIAMQEWLVNFGIPENEIDIQDTEKLL
                     EQWENNFDPDLNVKGMTYARRRYYQRVDAAIEGILSTEGATIAVENPETGALEPKDRV
                     EQSDYVNVSQVPSVRVLPLGTDNFGRDVLTELVAATRVSLQIGFVAGAIATLIGLVLG
                     LLAGYVGGPVDDAIMFVTNLFTVIPTIVLLILISFSIGQDMRGAFTIAVVIGLTAWFW
                     TARAVRAQVISLRNRDHVNLSKLSGHSISYIIIKDILPYIASYVVMALILQVSGAILA
                     EATLSIIGLGPRTTELPTLGLMMQWAMLYQAPLQGSWWAYFPVVLTIALIAFSMNLMN
                     TGLDQVFNPSLRE"
BASE COUNT          954 a         1015 c          888 g          978 t
ORIGIN      
        1 gattcataga gggcgtgcgc ggctgttgaa taggaactga ctgtggccag tgttgcaccg
       61 agccgttggg cgcggcgcaa gccttcgctc atgacagctt tgccgagccc gcgtttttga
      121 tgattcggat gcgtaccgac aggttcgaag accgcagtac gggtcacgtc gtcgaaccag
      181 actgtgcaga acgcggccaa ttcgccgtcc tcggcaaccg caacaatatc caggtcgcgt
      241 cggtagaggg gaacgcgttg cacgttgtaa taccactccc aactctcata tttttcgtcg
      301 ggctcatccg ggtgaaaaac tttccacgaa agccaactgc gcgcgggaag ttctctttcg
      361 tctcccaatg cgcggacggt gtatcccatc ttagagatgg aatcatgaat cccaccaaga
      421 gacgcggtcg actgtgaaat gaagcgatgg cgcatgtgtt cagctgaaaa ttccgaacga
      481 gcgtagcctt tttcaccgaa aagactcttt gttgatgtat ccgtcgcgtt cacccatacg
      541 atcaattctt ttttgtcacg gccgttgatc cttgagagct tctcttcggc tacttccaac
      601 atctcggaca tgaacactcg atcctgaaat tccgggtgga cctggaaaaa tgcttcgcca
      661 tctcgatctg ggttgagcac ggcaacgatc tgcgcgttca cctcccaaat tgtaatcaca
      721 tcctgtagat tgaagtgaaa gatattttcg ttgatgtgcc agcgccagta atcccaacgc
      781 aagagcgacc atgaaaaatc atgacgattg ttgtaaaggg atacctggcg cagcaattcg
      841 cggatgcgcc aatagtcttc ttcggattga tagtttcgtt gaattggata catagtcctc
      901 ttaggagaca gtcacttcga aagtgactgt ctcctggtat tccgaatcaa acctcacaca
      961 gttgagagat cccatacttc atgcatcttt ggaacgtcac taattaagcg ctgcgtgtat
     1021 gcctctttcg gatgcaaaat tacatcatca ggagttccgc tttcaataaa ttttccgtgc
     1081 tccataatgt agaccttatc cgatacgtaa taggccagac ccacatcatg cgtgatgaaa
     1141 ataagcgtca tgcctgtttc catatgcagt ttcattagca taccgaggat agtggcgcgg
     1201 caagaggcat cgatcatgga ggtgggctca tctgccaaca ggatcttcgg tttcagcagg
     1261 aagacccggg caatcatcaa acgttgcatc tgcccacccg agagctcaaa aggatatttg
     1321 ttggtcaatt cttcgaactt taagttgaca aagctgcatg cgtctgtcat cagcattact
     1381 tttttttcgt gggagagttt tcgaccgccg cgcatattaa tgcaatcgag cagtacggaa
     1441 tcaatcttat taaagatgtt ataggaagaa aatggatcct ggaagattgc ctgaatattt
     1501 ttccagtatt cctgcttctt ccggtatgtg ttgagattga gcttttttcc ctggaagtaa
     1561 atctcaccct ccgtggcgtt gatcaatccc aggatcattt tggccaacgt ggtctttccg
     1621 ctgccagatt cgcccacgat cgagacgatt tcgccttcat gaaaatcgaa atctacatga
     1681 tcgaccgcca ctgtcttatt ctgaccgaac ccgaaaatct tggtcagccc tgttccgctc
     1741 aggcatggat tcttctcatc agtcattttt acaggcctct ctcgcgtaaa tctctctcag
     1801 ttcgttttcc gacaccaggc agcgaaacgc ccgaccaccg tctgcctcgc gtaaagggat
     1861 ggaaagtacc ttacattccg gccgagcata tttgcaacgt tcggcaaacc gacagccggc
     1921 gggtgggttc ttaagattgg gtggtactcc aggaatagca gccagttcac attcgcgcaa
     1981 gcctttttcc ggtacgatga tcgagcccat gagcccccgc gcatacgggt ggattggatc
     2041 aaacaccgtt tctcctgcgg tacctttttc tacaatctgc ccggcataca taaccataat
     2101 gtcatccgtc acgtttttga gaagtggaag ctcatgcgtg ataaagatca acgatttgat
     2161 cagtcccttt tccatcagat ccctcagcat cttgatcacc atcttctgtg aggtgacatc
     2221 gagcgccgag gaaggctcat cagcaatcaa cacttttgga tagagaatca cggacacggc
     2281 gattaccgtt cgctgcttca taccgcccga aagctcaacc ggatattttt gcagcacatc
     2341 tgccgaaagc cccagaagct caaaacttcc tctcgcaagc tcataaatct cttttctggg
     2401 aacgtattca tcatgggcat gaattacatc ttcaataaag ttaataatct tccgggtagg
     2461 gtttaacgcg ttcattgcgg attgagggat ataggcaatc tccgatccca ggatcgattt
     2521 acgaacatta tcgggatcca ttcccgatat gttccgccca tcaatgttga tctctccact
     2581 cgtatagtac agtggaggaa agtaataacc cataaggctg agagccagtg tggattttcc
     2641 gcagccagat tcgcctgcaa tgcccagcga tttcccctcc tcgaccttca gcgaaacatg
     2701 gtcaaccgcg tacacatcct cctggtaacg ggtgatatat tttgtcgtca gttccttcac
     2761 atccagtatt acttttgaca tattactcac ctactctctc agggagggat tgaatacctg
     2821 gtcgagacct gtattcatca agttcatcga gaacgcaatc aaagcgatgg tgagaaccac
     2881 agggaagtat gcccaccagc ttccctgaag gggcgcctgg taaagcatcg cccattgcat
     2941 catcaaaccc aatgtaggca attctgtcgt ccttggtccc aatccgatga tcgacaaggt
     3001 ggcctcagcc aggattgcgc cagagacctg caggatgagc gccataacaa cataggaggc
     3061 aatgtagggc aagatgtctt taatgattat gtatgagatg gaatgcccgg ataatttcga
     3121 cagattcaca tggtcacgat tgcgcagcga aatcacttgc gcacgcaccg ccctggcggt
     3181 ccaaaaccag gcggtcaacc cgatcacgac cgcaatcgtg aaagcgccgc gcatatcctg
     3241 cccaatactg aacgaaatca ggatcagcaa cacgatggtg gggatcacag taaagaggtt
     3301 ggtgacgaac atgatggcat catccactgg accgcccacg taacctgcca gcaatcccag
     3361 aactaaaccg atcaatgttg caatcgcacc tgcgacaaag ccaatttgca gagaaacgcg
     3421 cgtggccgcc acaagctccg ttagcacatc cctgccgaag ttatccgttc cgagcggcag
     3481 gacccgtaca ctcggaacct ggctaacatt cacataatca gattgctcga ccctatcttt
     3541 cggttccagg gcgcctgttt ccgggttttc tactgcaatg gttgctccct ccgtagacaa
     3601 aattccttcg attgcggcat cgacccgctg gtagtatctt cgtctggcat aggtcatccc
     3661 cttaacgttc agatcggggt caaaattgtt ctcccactgc tccaggagtt tttcggtgtc
     3721 ctgaatatcg atctcatttt cgggaatgcc aaagttcaca agccattcct gcatggcaat
     3781 gcgatcttcg ctgctcaatt tgctggcaat tcgattggcg gcagcatcgt caaga
//
LOCUS       DASTJP010000383         3519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_556008, whole genome shotgun sequence.
ACCESSION   DASTJP010000383 DASTJP010000000
VERSION     DASTJP010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556008"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFQ23_11220"
     CDS             complement(<1..664)
                     /locus_tag="VFQ23_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907208.1"
                     /translation="MKTYILKPALFLWIVFSLLTGACGASPATEVPTSTATSTLVPTI
                     TPTITLTPTKTPTPTATPNLTATQQYNDFAALVQKIYDAGQISTTEGSYARVDDFSDA
                     LAMDYGYSWFPTGIQAKDFILRAQFDWEVANQKNFSGCGYIFRQESDQYYYLIVLDAI
                     NGVFLSYTKQGIASTGAGGIVNYTLAAAKKERLPDMGANPYQAEFTLVVNDNSAYTYV
                     NGD"
     gene            1091..2056
                     /locus_tag="VFQ23_11225"
     CDS             1091..2056
                     /locus_tag="VFQ23_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907209.1"
                     /translation="MRTIRLVALVIAATALVTPVTVFAPYFLHVQHFEGNPAHGYQAD
                     FYLYISPHAKQAAREGKLITILVQPNNSGINSDDPHVHRRDAWWTGFERRTIADELGV
                     ALLVPAFIRPREDWQIYTHTLDRDTLTTIRDDLRRVDLQLIAMIDYARSELATQNIQT
                     DPKILIQGFSASGMFANRFTILHPDRVRAATIGSPGGWPLVPVATFNDEPLPYPAGVA
                     DLELLTGIPFDFEVYNSIPQLIYMGSVDDNDSVDFTDGWDEPDAQLVDQLFGEDPLSR
                     WEDAESIFREAGANVQFLLIDGAGHDRKALQKYSTEFFKKVLSER"
     gene            2081..3022
                     /gene="mmuM"
                     /locus_tag="VFQ23_11230"
     CDS             2081..3022
                     /gene="mmuM"
                     /locus_tag="VFQ23_11230"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HET9907210.1"
                     /translation="MNPITSILNQHPALVIDGALATELERRGHNLKDSLWSAKILLEQ
                     PEAIKQLHYDYFKAGADCAITASYQATIEGFVKRGLNEKEAIGLIQKSVKLAIEARDE
                     FWADESHRIGRSKPFVAASVGPYGAFLADGSEYRGNYGLTERELMDFHRPRMKALIAA
                     GADMLACETIPSLIEAQALAKLLKEFPNRTAWFSFSARDETHISEGAPFEDCVRELEG
                     NAQVAAIGINCTSPKYIPSLIQEGKKSTAKPILVYPNSGESYNAVNQDWNGDPPHESF
                     GEEAREWYEAGARLIGGCCRTTPEDIQVIASWARGKL"
     gene            3092..>3519
                     /locus_tag="VFQ23_11235"
     CDS             3092..>3519
                     /locus_tag="VFQ23_11235"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9907211.1"
                     /translation="MKSVRMIEAGKPLELQETPIPEVGAKDILVRVRAAGICHSDAHY
                     RSGRSAMGFLPITLGHEVAGEVEGVGSEVTTVKVGERVCLHYNLTCGDCYYCSTGNEQ
                     FCDTVKMLGHHVDGGYAEYIVVPARNAIPLPKEIRYEEGAT"
BASE COUNT          935 a          860 c          845 g          879 t
ORIGIN      
        1 aatcaccgtt gacgtaggtg taggcagaat tatcatttac aaccaatgta aattctgctt
       61 gataaggatt tgcacccata tcaggcaatc tctctttttt ggcagctgcg agggtgtaat
      121 tcacaatacc tcccgctccg gtagaagcaa ttccctgctt ggtgtatgac aggaagacac
      181 cgttgatcgc atccaggacg atcaaataat agtattgatc tgattcctgt ctgaaaatat
      241 aaccacaccc ggaaaaattc ttctggttgg caacctccca gtcaaactgc gctcgaagga
      301 tgaaatcttt ggcttggatg ccagtcggaa accagctata cccatagtcc atagccagtg
      361 catccgaaaa atcatcaact ctcgcatacg aaccctcggt agtggatatc tgaccagcat
      421 cataaatctt ctggaccagt gccgcaaaat cgttgtattg ctgcgtagcc gtcaggttgg
      481 gagttgccgt tggcgtgggc gtcttggtcg gcgtcagagt aatcgtcggg gtgatcgtgg
      541 gcacaagcgt actggtggcg gtggaagttg gcacttccgt tgcagggctc gctccacagg
      601 ctccggtcaa tagactgaac acgatccaca ggaacagggc aggtttaaga atataggttt
      661 tcatttgatt ctccttttga tgaaatcttg acttgttcag attgtttcac cgccgcactg
      721 ccaattcaag atcattaact gccaaaaacc accacattga tcaaatgacc tgcgatcaat
      781 gcaaccacgt catgaaaaca gctataaatt gaatagttcc gggcgatatt gattcatagt
      841 cacctcctaa tcaatcaaga caacgataca cccaccatct gtaggataca actttctgtt
      901 tccaattaca agctcaaata tctcgtatat agttggctca ccgaatctga ttcaacataa
      961 aaaaaccgtt ccaacgaaaa tcatgaatct ttgaacagaa tggacgccat ggtctgaaag
     1021 tcctgatttg ttttaatggt atcctttggc agacgaatcc actctctgat aactggtcga
     1081 ggataattct gtgagaacca tcaggctcgt tgctctcgtc attgcggcca ctgcgctggt
     1141 tactcccgtc actgtgtttg ctccatattt cttacatgtg caacactttg aaggaaaccc
     1201 tgcccatgga tatcaggctg acttttatct ttatatctct cctcacgcga agcaggcagc
     1261 gcgagaaggc aagttaatta caattcttgt tcaacccaat aactctggaa ttaactcaga
     1321 tgaccctcac gtccaccgca gggacgcgtg gtggacaggt tttgagagac gaacaatcgc
     1381 tgatgaacta ggagttgcac ttctcgttcc tgcttttatc cgcccccgtg aggattggca
     1441 aatctacact cacacactag accgtgatac gctcacaacc atacgcgacg atcttaggcg
     1501 ggttgatctc caattgattg caatgattga ctatgcgcgc tctgagctgg caacgcagaa
     1561 tattcaaact gaccccaaga ttttgattca gggattctct gcaagcggca tgtttgccaa
     1621 tcggtttaca attttgcatc ccgaccgtgt cagggctgca acgattgggt cgcccggtgg
     1681 ctggccacta gtgcccgttg caactttcaa tgatgagcct ctcccctatc ctgcgggtgt
     1741 cgccgatctc gaactattga ctggtattcc atttgacttt gaggtctata actcaattcc
     1801 gcaattaata tatatgggct ccgttgatga caatgactcg gtagatttca ctgacggctg
     1861 ggatgaaccc gacgcgcaac tcgtagacca gctctttggc gaagatccgc tttcgcgctg
     1921 ggaggatgcc gagtcgattt ttcgagaggc tggagcaaac gtccagtttc tacttattga
     1981 tggagccggc catgaccgca aagctctaca aaaatactca acagagttct tcaagaaggt
     2041 actaagtgag cggtaggtga ctgtcacctg tttaaaaatt atgaacccca tcacctctat
     2101 tctcaaccaa catcctgccc tcgtaattga cggcgcgctc gcaaccgaac tcgaacgccg
     2161 cggtcataac ttgaaagatt cactatggtc tgcaaaaatt ttgttggagc aacctgaagc
     2221 catcaaacag ctccattatg attacttcaa ggcaggcgcg gattgtgcca tcaccgctag
     2281 ttatcaagcc acaatagaag gttttgtgaa gcgtggtttg aatgaaaaag aagccattgg
     2341 gttaattcaa aaatccgtga aattagctat cgaagcacgt gacgaattct gggccgacga
     2401 atctcaccga attgggagat cgaagccctt tgttgccgcc tccgtcggac cctacggggc
     2461 atttctagcc gacggctcag agtatcgcgg caattacgga ttgacagaac gggaattgat
     2521 ggactttcac cgaccgcgta tgaaggctct gatcgcagcc ggtgcagata tgctggcttg
     2581 tgaaaccatt ccttctctca tcgaagcaca ggcactggca aagttactca aggaatttcc
     2641 gaacaggaca gcctggttca gcttttccgc acgagatgaa acgcacattt cagaaggtgc
     2701 gccctttgaa gattgtgtaa gagaattgga agggaatgcg caagttgcag ccattggaat
     2761 caattgcact tcaccaaaat atatcccgtc tttaattcag gaagggaaga aatcgacggc
     2821 aaaaccaatt ctggtctatc ctaattcagg cgaatcatac aatgcagtca accaggattg
     2881 gaatggcgac cctccgcacg aatcatttgg tgaggaagca cgagaatggt acgaggcggg
     2941 cgcgcgccta atcggcggct gttgccgcac aacgcccgag gatattcaag tcattgcgag
     3001 ctgggcaaga ggcaaattgt agggaccggc agctcgcgaa gtaccctgat gagggtgccc
     3061 gtccctacaa ttgataatta ggagaaaaca gatgaaatcc gttcgaatga tcgaagcagg
     3121 caagcccttg gagttgcagg aaaccccaat accggaggtc ggtgcaaagg atattctcgt
     3181 gcgcgtgcgt gctgcgggaa tctgtcattc ggatgcgcat taccgctccg gccggtcagc
     3241 catgggattc ctgccgatca cactcggaca tgaggtcgcc ggtgaggttg aaggagtcgg
     3301 ttcagaggta accaccgtaa aagttggaga gcgtgtttgc ctgcattaca acctcacctg
     3361 cggcgattgt tattattgca gcacagggaa cgaacagttc tgcgacactg taaagatgct
     3421 tggtcatcat gtggatggtg gttatgcaga atatattgtc gttcccgccc gcaacgcgat
     3481 ccctttacca aaagaaatta gatacgaaga aggtgcaac
//
LOCUS       DASTJP010000384         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_875134, whole genome shotgun sequence.
ACCESSION   DASTJP010000384 DASTJP010000000
VERSION     DASTJP010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875134"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFQ23_11240"
     CDS             complement(<1..722)
                     /locus_tag="VFQ23_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HET9907212.1"
                     /translation="MTVTAVIGSQWGDEGKGKVVDYLAERADYVARFNGGNNAGHTVI
                     NEFGTFKIHLVPSGIFARNAVGLIGGGVVIDPSVLIEEIDLLNNAGVGVEGRLWISPR
                     SHLIMPYHKILDGLYEEAKGAGATGTTRRGIGPVFADKVSYNGIRWSDFASKAFESRL
                     QVQLELKNKIIAALGGEALKYEEVRDTYREYYQRLKPYIRELFSLVQDGLKAEKHFLL
                     EQAMGTFLDTDWGTYPFVTASTT"
     gene            complement(745..2079)
                     /gene="purB"
                     /locus_tag="VFQ23_11245"
     CDS             complement(745..2079)
                     /gene="purB"
                     /locus_tag="VFQ23_11245"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET9907213.1"
                     /translation="MTLHALSPLDGRYENEVAPLREYFSEFAYLRARVRLELDYLSAL
                     TKIGVISSLSRSDWQSDLQGFTEEDALKIQEYEKTTRHDVKAIEYYLRERLPSELHQY
                     IHFGLTSEDVNNIAQAIALRDSRDRVLLPAFDQLLNSLRDFAQKYRALPMLARTHGQP
                     ALPTTLGKEIAVYLARLNKCRDEIAAYKFEAKLTGAVGNFNALQAAAPQVDWISFSKE
                     FIESLGLEPNLITTQILPYDNWIRYFDSTKLANAILIDYTQDVWRYISEGYLKQKAVE
                     GEVGSSTMPQKVNPIDFENAEGNLGIANALLTHYSQKLPISRLQRDLSDSTVRRTFGV
                     ALGHTLLAWNNITRGMSRVDADEERIKSELNGHWEVVGEGAQTILRAAGQSDAYESLK
                     SQTRGLVLTEADFKSWIKTLDIDDQTRIRLEGLSPESYLGLAIQIVDDILDS"
     gene            2159..3598
                     /gene="guaB"
                     /locus_tag="VFQ23_11250"
     CDS             2159..3598
                     /gene="guaB"
                     /locus_tag="VFQ23_11250"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET9907214.1"
                     /translation="MKILPEMALTYDDVLLVPQYSEVDSRRALSTKGCLTKKIALQVP
                     IVSANMDVVTESEMAITMAREGGIGMIHRFMTIAEQARQIERVKKAESFIVDKPIKMT
                     EAHTVGDVKRIVEETGTGGILIVDKHEKLIGIVSTRDLLFEEDDDKPVTEIMSREVHS
                     AAPDTTLKEAERLLHEFRVEKLPLVDTDGKVAGLVTLKDIMKITQFPKATKDARGRLS
                     VGAAVGVRDKEMRRVEAVLEAGADCIVVDIAHGDSHLEIEMIKNIRTHFPNAQIIGGN
                     VATSEGTKRLIEAGVDAVKVGVGPGSICITRNVAGSGVPQLTAVIECARAAKPYGVPI
                     IADGGIRQPGDVVKAIAAGAHTVMIGSMLAGTDESPGMIMTRRGHRYKASRGMASLEA
                     NINRNRREGNDLTHEEIEDYVAEGVEAAVPYRGRAREVLNQLVGGLQSGMSYSGAHSL
                     DELQEKAIFVRMTGAGLRESGPHDVEVIG"
BASE COUNT          918 a          881 c          952 g          930 t
ORIGIN      
        1 gtcgttgaag cagtcacaaa tgggtaggtg ccccaatctg tatctagaaa agttcccatg
       61 gcttgttcga gcaagaaatg tttttctgct ttcaagccat cctgcaccag cgagaacaac
      121 tcgcgaatat atggcttcag gcgttggtaa tattcacgat aggtatcgcg cacttcctcg
      181 tatttcagcg cttccccgcc taacgctgcg atgatcttat tcttcagctc cagctgcact
      241 tgcaagcgtg actcaaatgc cttgctcgca aaatcgctcc agcggatgcc gttgtagctc
      301 accttgtctg caaaaaccgg cccaatgccg cgccgcgttg tcccggtggc gcccgcgccc
      361 tttgcctctt cgtacaagcc atccaaaatt ttgtgatagg gcatgatcaa atgcgagcgc
      421 ggtgaaatcc ataaacgacc ttcgacgccg acacctgcgt tgttcaacaa gtcaatctct
      481 tcaatcaaca cggaagggtc aatcaccaca cctccaccga tcaaaccgac cgcattgcga
      541 gcaaaaattc cggaaggcac aaggtggatc ttgaatgttc caaactcatt gatcaccgtg
      601 tgccctgcat tattcccgcc gttgaaccgc gctacataat ctgcgcgctc cgctaagtaa
      661 tctacaactt ttcccttacc ttcatcaccc cattgcgaac cgatcactgc tgttactgtc
      721 atctcgtctc ctagtctact tgtctcaact atctaaaatg tcatccacaa tctgtatcgc
      781 tagtccaaga taggactccg gtgataatcc ttccaaacgg attctcgttt ggtcatcaat
      841 atccagcgtt ttaatccagg atttgaaatc tgcttccgtt aaaacgagac cgcgcgtttg
      901 agactttagt gactcgtacg cgtcactttg cccggcggcg cgtaaaattg tctgcgcgcc
      961 ttcgcctaca acttcccaat gtccgttcaa ttcacttttg attctttctt catctgcatc
     1021 cacacgactc ataccgcgcg tgatgttatt ccatgcaagt agagtgtgac ccaaggcgac
     1081 tccgaacgtt cgccgaactg ttgaatccga tagatctctc tgcaatcgag agatcggcag
     1141 cttttgacta tagtgggtga gaagtgcgtt tgcgattccg agattgcctt cagcgttttc
     1201 aaagtcaatc ggattcacct tttgaggcat cgtcgaagac cccacttcgc cttcgaccgc
     1261 tttttgcttt aagtatcctt cactaatgta tcgccacacg tcttgcgtat agtcaataag
     1321 tatggcgttg gctagtttgg tggaatcaaa ataacgaatc caattgtcat aagggaggat
     1381 ctgagttgtg attagattgg gttccagtcc aagactttca ataaactctt tgctgaacga
     1441 aatccaatcc acttgtggtg ctgcagcttg cagcgcgttg aagttgccaa ctgcacccgt
     1501 gagctttgcc tcgaatttgt aggcagcgat ttcatcgcgg catttattca aacgcgccag
     1561 gtaaactgcg atttcttttc ccaatgttgt cggcaaagca ggctgaccgt gagtccgtgc
     1621 cagcatgggg agggcacgat atttctgtgc gaagtcccgt agcgaattga gaagctgatc
     1681 gaatgcgggg agtaggacgc gatcgcgcga gtcgcgtaag gcgattgctt gcgcgatgtt
     1741 gtttacatcc tcagaagtga gtccgaagtg aatatattga tgaagttcac tggggagtct
     1801 ttctcgtaag taatattcta tggctttgac atcatgtctt gttgtctttt cgtattcctg
     1861 aatcttgagc gcgtcttcct cggtgaaacc ctgtaggtcg gattgccaat ccgacctact
     1921 caaggaagag ataacgccta ttttagtgag agcagataaa tagtccagtt cgaggcggac
     1981 gcgggcacgt agataggcga attcagaaaa atactcgcgc agaggtgcga cctcgttctc
     2041 gtaacgcccg tccaggggag aaagcgcatg cagtgtcatt tgcaaggatt ataattcgtg
     2101 cggtttattc taataagact tccgaagtct ccaagacttc ggaagtctgg aggttgaaat
     2161 gaaaattttg cccgaaatgg ctttgactta cgatgatgtt ttattggtac cgcaatattc
     2221 tgaagtcgac tcgcggcgcg cgctttcaac caaagggtgc ctcacgaaaa agattgcttt
     2281 gcaggtgcca attgtctccg ctaatatgga tgtagttacc gagagcgaga tggccatcac
     2341 tatggcgcgc gagggcggca ttggcatgat ccatcgcttt atgacaattg cagaacaggc
     2401 gcgacagatc gagcgtgtaa aaaaggccga gtctttcatt gtggataaac ctatcaaaat
     2461 gaccgaggca catacggtcg gtgatgtgaa gcgcatcgta gaggagacag gtacgggcgg
     2521 aattttgatt gtagataaac atgagaaatt gattggcatc gtctcgacac gcgatttact
     2581 tttcgaagag gacgatgaca aacctgtgac cgaaatcatg tccagggagg tccatagtgc
     2641 tgcacctgat accacattga aagaagctga acgtttgttg catgaatttc gggttgagaa
     2701 gctgccgctt gtggatacag atggcaaggt agctggtttg gtcacgttga aagatattat
     2761 gaagatcacg cagttcccga aagcaacaaa agatgcgcgt ggtcgcttgt ccgtgggtgc
     2821 tgccgtgggc gtgcgcgaca aggaaatgcg ccgcgtggaa gctgtgctgg aggccggggc
     2881 agattgtatc gtcgtggata tcgcgcacgg cgattcgcat cttgagattg aaatgatcaa
     2941 aaatattcgc actcactttc cgaatgccca gattattggc gggaatgttg ctacctcaga
     3001 agggacgaaa cgcttgatcg aagccggagt cgatgctgta aaagtcgggg tgggtccagg
     3061 ttctatttgc attacacgga acgtggcggg ctcaggagtc ccgcaattga cggctgttat
     3121 agaatgtgcc cgagccgcca aaccgtatgg cgttccaatc atcgcagatg gagggattcg
     3181 ccagccaggt gacgtcgtaa aggccatcgc ggccggtgcg cacactgtga tgattggttc
     3241 catgctggca ggtacggatg aaagccctgg aatgatcatg acccgcaggg gacaccgtta
     3301 taaagcctcg cgggggatgg catcgcttga agccaatatc aatcgcaatc ggcgcgaagg
     3361 caacgacttg acccatgagg aaatcgagga ttatgttgca gaaggtgtgg aagccgctgt
     3421 cccttatcgc ggcagggcac gcgaagtgtt gaatcaattg gtaggtgggc tacaatcagg
     3481 aatgagctat agcggtgcac attcgttgga cgagttgcaa gagaaagcaa tctttgtcag
     3541 aatgaccggc gcgggattga gggagtcagg cccgcacgat gtggaagtaa ttggatgatc
     3601 cgatttcctg atacatcaat cggaatgacg actaaaacgg agacaacatg accaaccagg
     3661 ctacagcaac ttggatcgaa a
//
LOCUS       DASTJP010000385         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_956536, whole genome shotgun sequence.
ACCESSION   DASTJP010000385 DASTJP010000000
VERSION     DASTJP010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956536"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFQ23_11255"
     CDS             complement(<1..737)
                     /locus_tag="VFQ23_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907215.1"
                     /translation="MKIKIPLFLYCLLTLCVAGCSIDVAQSVVPTPSLPVGSILPTQS
                     VSSENGTPSVAGTSVPVTWAGLNLTGKLLYASVPLTSVTPISIHTLDLATGEIQTLFT
                     TTGDAWVYYMTVSPDGKQVIMSYAPPSEGPIPSSRALYTMPTDGSREPELLFQPPTPD
                     DHYIHVEWSSDGKYIYYVHYNNTERAAGELYPAYELLRMTYPDGQPESIAQKAFWPRL
                     SADSSRLVYVSLDPATGLNELFLANADG"
     gene            complement(803..>2137)
                     /locus_tag="VFQ23_11260"
     CDS             complement(803..>2137)
                     /locus_tag="VFQ23_11260"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET9907216.1"
                     /translation="AATEVRPLIPMLGDWLHLIGMTFWLGGLIYLFTGLRKLRQLDTS
                     GNAKLISSLTSRFSVNAIFFVALIGVTGFYSAYLRVGSWSAALTSLYGHVLLIKQIFV
                     AGLLTIAATNLLVISPKLKSNAIQEVAEPSIVTRFGKLLALELTLAGLLLGSVSFLTY
                     IPPAKAVSFNTDLTDRTQVDDLRMDVNISPGRVGQNTFTLHLSTEGQPLGSAKEVLLR
                     FTPTQANVPPSELELINTGDGSFTAKGVHLSLQGDWQVQAVVRREDKFDAFANFNFTV
                     RNPGSAESSSMVPRQAAWLILLIGLLCSALTASVKAHPALRLGIGAPLAILMLGLGIF
                     YVRLPVSIVNVANPIPPNSESIATGQAIYTTHCASCHGPEGNGDGPIGVTLNPRPADL
                     SQHAIPGVHMDAQLFEWITNGFPGSRMPAFKTALSDTDRWHLVNFIRTFAPK"
BASE COUNT          577 a          537 c          574 g          449 t
ORIGIN      
        1 ccgtctgcat tggcgagaaa caattcattg agacccgtgg cggggtcaag ggatacgtaa
       61 accagccggg acgagtcagc agaaagtcgc ggccagaatg ctttttgcgc gatcgattca
      121 ggctgaccat ccggatacgt catgcggagc agttcatacg ccgggtaaag ctcgcctgcc
      181 gccctctccg tgttgttgta atgaacataa taaatatact ttccatctga cgaccattcc
      241 acatgaatgt agtgatcatc cggggtggga ggttgaaata gcaattccgg ttctctcgat
      301 ccgtcggtag gcattgtata caaggcccga ctcgaaggaa tcggaccttc tgatggcggt
      361 gcataggaca tgatcacttg tttcccatcc ggggaaacag tcatgtaata gacccaggca
      421 tcacccgtgg ttgtaaataa agtctggatc tcccctgtgg cgagatccag ggtgtgaatg
      481 ctgatggggg taacacttgt tagcggcacg ctggcataca ataattttcc ggtcaagttc
      541 aggcctgccc atgtgaccgg aacagacgtg ccagcgacag aaggagtgcc attctccgag
      601 ctcacagact gcgtaggcag gatggatcca acgggtaacg acggtgtcgg tacaactgac
      661 tgagcaacat caattgaaca accggctaca caaagtgtca acaagcaata cagaaacaga
      721 ggaattttta ttttcatatt aagcgatcca gataagtagg gcgaggaaac cgcgtaattc
      781 agaagcgaga aattttctga gattacttcg gcgcgaaggt acgaatgaaa ttgaccagat
      841 gccagcgatc cgtatcggag agcgccgttt tgaaggcagg catgcgcgac ccgggaaagc
      901 cattcgtgat ccattcgaat agctgtgcat ccatgtgaac accagggatg gcatgttgac
      961 tgagatccgc ggggcgtggg ttaagagtca cgcctatggg cccatccccg ttcccttccg
     1021 ggccgtggca ggaagcacaa tgggtggtgt atatggcctg gccggttgcg attgattcgc
     1081 tgttcggggg aattgggttc gccacgttca caatggagac tggcaatctg acataaaaga
     1141 tacccaggcc cagcatcaaa atggcaagcg gtgcgccgat ccccaaacgt aacgcgggat
     1201 gcgctttgac ggaggcagtg agcgcgctac ataggagccc gatcagcaaa atgagccagg
     1261 ccgcctgccg cggaaccatt gaagaagact cagcacttcc gggattgcga acagtaaagt
     1321 tgaaatttgc aaaagcatca aacttatcct cacgccgcac gactgcctga acctgccaat
     1381 cgccctgcaa actgaggtgc acaccctttg cagtaaaact gccatcccct gtattgatca
     1441 attccagttc cgagggcgga acgttcgcct gggtgggtgt gaagcgcaat aagacttctt
     1501 ttgcagatcc cagcggctga ccctctgttg acaaatgtaa agtgaacgta ttctgaccaa
     1561 cccgacccgg agaaatgttg acatccatgc ggagatcgtc tacttgtgtt cgatccgtga
     1621 ggtctgtatt aaaagaaacc gcttttgcag gtggaatgta cgtgaggaaa cttacgctac
     1681 ctaatagcaa accagccagg gtcagttcga gcgccaacag ctttccaaag cgggtcacaa
     1741 tcgaaggttc cgcgacctcc tggattgcgt tactcttaag ctttggcgaa atcaccagaa
     1801 ggttagtcgc cgcaatcgtt agtaaacctg cgacaaatat ctgcttgatc agcaggacat
     1861 gtccatagag actggtgagc gcagcggacc aggaacccac ccgcaaatag gcggagtaaa
     1921 aaccggtgac cccaatcaac gcgacaaaga aaatcgcgtt gactgaaaat ctcgaggtca
     1981 gcgaagaaat caatttcgcg tttccactgg tatccagttg ccgaagttta cgaagcccgg
     2041 taaacaagta aatcagcccg cccagccaaa aggtcatgcc aatcaaatga agccaatcgc
     2101 caagcattgg gatcagggga cgcacctcag tggcagc
//
LOCUS       DASTJP010000386         4321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1316914, whole genome shotgun sequence.
ACCESSION   DASTJP010000386 DASTJP010000000
VERSION     DASTJP010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316914"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..448
                     /locus_tag="VFQ23_11265"
     CDS             92..448
                     /locus_tag="VFQ23_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907217.1"
                     /translation="MDDYKSIGIYLEKEEKERVDTFLIHVDHLFRLTLSEKQKKIYDA
                     YRATQIFKDNGMTIKGYLEFIRVTSELTILTGIPFSLESDFAGLDSLREWDAVITKAF
                     EAAKVDQKRKKRKTTK"
     gene            546..1172
                     /locus_tag="VFQ23_11270"
     CDS             546..1172
                     /locus_tag="VFQ23_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET9907218.1"
                     /translation="MIDPAKFILFIGVSWALIIAPGPDMIYVITRGVTQGRRAGMLSA
                     VGVICGILVHTTAAAFGLTLILQTSALAFMLVKYLGAIYLIYLGIKAWGDKSTFRLDP
                     STTTVSFRKIFWQGVLSNVLNPKIAIFFLAFLPQFVDQGNGVTLQMVVLGLTFAFFGL
                     CFLSVVGYSSGTIGSWLTHRPRIGQILGRFAAGILIALGIRLALTERT"
     gene            1262..1483
                     /locus_tag="VFQ23_11275"
     CDS             1262..1483
                     /locus_tag="VFQ23_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907219.1"
                     /translation="MAYTLDTKVGEILDDTNAVEVLERYVPGISKNPMLAFAKGMALK
                     SILDVPQAKEAGITEEMVLKVLAEINARK"
     gene            1619..2305
                     /locus_tag="VFQ23_11280"
     CDS             1619..2305
                     /locus_tag="VFQ23_11280"
                     /inference="COORDINATES: protein motif:HMM:NF039337.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HET9907220.1"
                     /translation="MPSSTAYSLLEACRAPGCPVCRLEQRAVERYLDNKFYESVNSPA
                     WRDELRQSFGFCHEHSWLAVTERLGDALGFSIISRDLVNSIQRQLTEEMSSSASTGRR
                     TSVLNRILAALTPRKRCPVCEHRDETTRRIISALVEELDQPNMTDALESSDGLCLPHL
                     RLVLEQVKDVSDREALLTIHLDKLESLRAELDEFIRKSEYQAVREGFGSEGNAWQRAV
                     SMIVGARKEK"
     gene            2309..3442
                     /locus_tag="VFQ23_11285"
     CDS             2309..3442
                     /locus_tag="VFQ23_11285"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9907221.1"
                     /translation="MANEEQLRILKQGVEAWNQWRKDHADTKVDLSGSDLSGLNLTGV
                     DLRDARLTRVDLREANLNSARLSLSNLSRASLIGASLHQATLRETRLSQTIIMKSDLR
                     DADLSGAYLQKTVLASNDLTGAYLYNAQLSDVYLNETKFTNAAFAATTFGDTDLSIAN
                     DLETASHHGPSTIGLDTIHKSKGNIPIGFLRGCGLSDADIEFAKLSNPDFSNEQINKI
                     LYRVYELRASQAVQISPLFISYSHADGLFVDKLDEHLNKKGIRFWRDVHDMKSGKIET
                     QIDRAIRQNPTVLLILSEHSLQSDWVQHEVRTARELEKDLSHDVLCPVALDDSWKNSS
                     WPRRIMEQVMEYNILDFSAWKEDDKFGVTFKKLIDGLQLFYKG"
     gene            3445..>4321
                     /locus_tag="VFQ23_11290"
     CDS             3445..>4321
                     /locus_tag="VFQ23_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HET9907222.1"
                     /translation="MTKQIHTLKGAIDVDSLGLILPHEHLFTDLRGPLVDGYAQAEPS
                     EVARLVGPFLADAFEAGVTTLVECSTLGVGRNLAVLKHLAEASPIHIVAPTGVYRDAF
                     IPPALRVMNETDLARLWIDELTKGIENTSIRAGFIKLAMSDDGPTDLEKRNLRAAAQA
                     SKATGAVIASHTIGGKVAKEEMDVLEAAGHDLQRFIWIHAQTEPDVTVLKEAAARGAY
                     IELDSVGAPNQSDAELADTTLALMEAGYLDHILLSHDAGWYNPGRPDGMPDHGFHGYT
                     ALIREFLSALSARGIS"
BASE COUNT         1159 a         1001 c         1096 g         1065 t
ORIGIN      
        1 acgcctgaag ttctcagtaa tgaacttgtc atgactttgc atttgtagga gattgcggtt
       61 tcttgtatgc tctccataga ggcatacaga aatggatgac tacaagagca taggaattta
      121 cctggaaaaa gaagaaaaag agcgagtcga tacctttttg atccatgtgg accatttgtt
      181 tcgattgacg ctttctgaaa aacaaaagaa aatttacgat gcctaccgag ccacgcaaat
      241 atttaaagat aacggcatga cgattaaggg ctatctggag ttcatccgtg tcacaagcga
      301 actcacaatt ctcaccggca tcccattttc attagaatcg gattttgcgg gcttggattc
      361 gcttcgcgag tgggacgccg tgatcaccaa agcctttgaa gcagccaagg ttgaccaaaa
      421 gcgcaagaag cgcaagacaa ccaaatagat aattactccc cctaagcgac agcacatcca
      481 gcagcgactt tcagagttac taaagttgag gtacgattag atattgtttt cgcgtatgag
      541 gcagcatgat tgatccggca aaatttatct tattcatcgg cgtctcctgg gcgttgatca
      601 ttgcaccggg acccgacatg atctatgtca tcacgcgcgg tgtgacgcag ggacgcagag
      661 ccgggatgct ctccgctgtc ggcgtgatct gcgggattct cgttcacaca actgccgccg
      721 cctttggcct gaccctgatc ctgcaaacat ccgcactggc ttttatgctg gtcaaatatc
      781 ttggagcgat ctatttaatt tatcttggta tcaaagcctg gggagataag agcacgttcc
      841 gtctggatcc ttcaacgaca accgtcagtt ttcgtaagat cttctggcag ggagtcctat
      901 cgaatgtgct caacccaaaa atcgccatct tctttttggc attccttcct cagtttgtgg
      961 atcaaggcaa cggtgtaacg ctacaaatgg tcgtactggg tttaacattt gccttctttg
     1021 ggttgtgctt cttatccgtt gtgggatatt catccggaac cattggcagc tggctcaccc
     1081 accgaccccg tatcgggcag atcctggggc gtttcgccgc tggtattttg atcgcgctcg
     1141 gaatacggct ggcgctcaca gagcgaacgt aagtccgtga gcattgaata gacgatctta
     1201 aagcacgtat aatacgatta gtatttgatg ttctaaaaat caatcaaacg aggagagcaa
     1261 aatggcatac acattggaca caaaggtcgg tgaaattctg gacgacacga atgccgtgga
     1321 ggtcctggaa cgctatgttc ctggcatttc caaaaatcct atgcttgcgt tcgcaaaagg
     1381 aatggcatta aagtccattc tggatgtgcc gcaggcaaaa gaagcgggca tcactgaaga
     1441 aatggtgcta aaagtattag cagagattaa cgctcggaag taacgaagtc agttccgcac
     1501 aaattttgtg tttttgatca ccttgagcgc tgtccctttc ggagtagcgc tcaattcttt
     1561 tcacaacaat tgaagtaaac tctagtctga acacgcccag gaaaggaaat aatatctttt
     1621 gccaagctct actgcctaca gcctattgga agcctgccgc gcaccgggct gccctgtttg
     1681 caggttggaa cagcgcgccg tagaacgcta tctggataat aagttctatg aaagtgtgaa
     1741 ctcacctgcc tggcgtgacg aactgcgtca gagcttcgga ttctgtcacg aacattcctg
     1801 gctggcagtt actgaacgac tgggcgacgc gttgggattc tcgattattt cccgcgatct
     1861 ggttaattcc atccaaaggc aattgactga ggaaatgagt tcgtcagcga gcacaggacg
     1921 acgaacatct gtgctcaacc ggattcttgc cgcgttgact cctcgtaaac gctgtcctgt
     1981 ctgtgagcat cgagacgaaa caactcgcag gatcatatct gcattggtgg aagagctgga
     2041 tcagccgaat atgacggatg ctcttgaatc gtccgatgga ctttgtctgc cacatttgag
     2101 actggtgcta gagcaggtga aggatgtatc tgacagagaa gcacttctca cgattcacct
     2161 tgataaactg gagagcttgc gcgccgaact ggatgagttc atccgtaaaa gtgaatatca
     2221 agccgttcgc gagggctttg gttcagaagg aaacgcctgg caacgagccg tcagtatgat
     2281 tgtcggcgcg agaaaagaaa aataagatat ggcaaatgag gaacagctaa gaattttgaa
     2341 gcaaggcgtc gaggcatgga atcagtggag aaaagaccat gccgatacca aggttgattt
     2401 aagtgggagt gaccttagcg ggctgaacct gacgggagtc gatctgagag atgcccgtct
     2461 cacacgcgtg gaccttcgag aggcaaatct gaatagcgct cgcctttcgc tatcaaacct
     2521 gagcagagcg tcattaatcg gcgcatcgct acatcaagcg accctcaggg aaacgcgtct
     2581 atctcaaaca atcattatga aaagtgatct gcgggatgct gatctttcag gtgcttatct
     2641 gcagaaaacc gttctcgctt caaacgacct cacgggggca tatctctaca acgcccagct
     2701 gagtgatgta tatctcaatg aaacaaaatt tacaaatgct gcatttgcag ctacaacatt
     2761 cggtgacact gatcttagca ttgcaaatga ccttgagaca gcgagtcatc acggaccatc
     2821 caccattggg ctggatacaa tccacaaatc aaaagggaac atcccaatcg gttttctgcg
     2881 cggctgcggt ctcagcgatg cagatattga atttgcaaaa ctttcgaatc cagattttag
     2941 taatgagcag atcaataaga tcttatatcg tgtctacgaa ctaagggcga gccaggcagt
     3001 tcagatttct cctttgttca tttcatacag tcatgccgat ggcctctttg tggataaact
     3061 ggatgagcat ctaaacaaga aaggcatccg tttttggcgg gatgtgcatg acatgaagtc
     3121 cggcaaaatc gaaacacaaa tcgatcgcgc catccggcaa aaccccacgg tgttgttgat
     3181 cctctcggaa cattcacttc aaagcgactg ggtgcagcat gaggtccgca cggcgcggga
     3241 gctggaaaaa gacctatctc acgatgtgtt gtgtccggtg gcattggatg acagttggaa
     3301 gaacagctct tggcccaggc ggattatgga acaagtaatg gaatataaca ttctggattt
     3361 ttccgcatgg aaagaagatg ataagtttgg ggtaacattc aaaaaattga tcgatgggct
     3421 tcaattattt tataagggct gaccatgacg aaacaaatac acacgctcaa aggcgcgata
     3481 gatgttgact ctcttggctt gatcttgccg catgaacatc tgtttacaga cttgcgcggt
     3541 ccgctcgttg atggctacgc tcaagctgaa ccttcagagg tggctcgcct cgtagggcct
     3601 tttttagccg acgcgtttga agcaggtgtg actacattag tggaatgttc gactctaggc
     3661 gtcggacgaa atctcgcagt attgaaacat ctggcagagg cttcaccaat tcatatcgtc
     3721 gcaccgactg gcgtgtatcg ggatgctttc atcccgccgg ccctccgtgt aatgaacgaa
     3781 actgacctgg cgagactttg gattgatgaa ctcacaaagg gaatcgaaaa tacttcgatt
     3841 cgagccggtt ttatcaaact ggccatgagc gatgatgggc ccacagacct ggagaaacgg
     3901 aatctgcgag ccgcggctca ggcaagcaaa gcgacaggcg cggtcatagc cagccataca
     3961 atcggcggaa aagttgccaa ggaagagatg gacgttctgg aagcggctgg gcacgattta
     4021 caacgattta tctggatcca tgcccaaaca gagccagacg tgaccgtgtt gaaagaggca
     4081 gccgcgcgcg gcgcatacat cgaattggat tctgtaggag cgccaaatca atcggatgca
     4141 gaattagccg atacgaccct ggctcttatg gaagcgggtt atcttgatca tattttgcta
     4201 tctcacgatg caggttggta taacccgggt cgccccgatg gaatgccaga tcatggattc
     4261 cacggttata cggcactgat aagggagttt ctctcggcat tatctgcgcg gggaatcagc
     4321 g
//
LOCUS       DASTJP010000387         7195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_350994, whole genome shotgun sequence.
ACCESSION   DASTJP010000387 DASTJP010000000
VERSION     DASTJP010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350994"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VFQ23_11295"
     CDS             <1..1129
                     /locus_tag="VFQ23_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907223.1"
                     /translation="RMNKRHILYHCTVIVLAMNLSCTTQSPTSLPVEQPTAVSTALAS
                     STPPSEATQENASVATVTAVIAVTGHLMKPAELAPKPGNLIYDVESSGIAAPYGDLFK
                     LNRLERPFLADMTYVSDMDITSFNLSEDSDWYYVSIELSGNDPNNSIGINYGVEIDLD
                     FDGFGDYILWSQPPYSTSWETTNLKVFKDSDHDSAGLSGTQSDTGLGGNGYDELVFDG
                     EGGQNDDPDLAWVRVNTEPNAVVQFVFKKSLVGSFFMLGVVSDAGLKDISKFDYNDSF
                     EEAEAGSPDKNKAYYPLGSLYGVDNTCWEAYGIQTTGYEPKLCQPILQPTPTFQPFQP
                     LQPSIPTDEGFDCIPDVPPDSCGFDEETGAPYYNPATCTCT"
     gene            complement(1193..5002)
                     /locus_tag="VFQ23_11300"
     CDS             complement(1193..5002)
                     /locus_tag="VFQ23_11300"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907224.1"
                     /translation="MNDANDKDEAKVQADDHSIAIGGISVGGNVGDIRVGHTIGFTAD
                     QVSVLLTQIKSEFQVKPFDGRCPYKGLDVFEEEDAEFFFGREKLVDELVTRVKESRTL
                     FVTGPSGSGKSSLVRAGLIHALKEGALKNSQRWMYETMKPGRDPIGELGRVASSLASS
                     TNAEDEIRAKASADPTILARWCEIALKDSRDKRAVIFIDQFEEVFTQINKEEERKAFL
                     NLLTHTASVENGRVILLFAMRSDFLSNCATYTDLNALLNEQFIQIGAMQPDELVSAMA
                     QPALRVGLRIDPDLIAQVINDMKGEPGALPLMQFTLKDLFDSQHAKGGLISLTLDDYL
                     RHGGIQKSLERHADAAFAKLELNEQELARSIFTGLIEIGRGTQDTRRTALFDELIPAN
                     ANAQVVEVIVQKLADARLIITDEQAGKDTVTISHEKLIDAWPWLKKLVNDNREVIALQ
                     NQIAEDAKEWDEQKRDPSYLYSGARLVNVQEQLATEKLVLSNMAQEFIRAGNVKRRRN
                     QAAVVGGVSTIIALLLIAVVVFINQSNRYLKIAGTAQAASTEAVSQRATAESNAEEAQ
                     RQQQIAHVREIVAQSTALRERNLALSLLLGMEAFKNENTYQTRSVLLENLQFNSQLLE
                     FLVGHSDSVTSVAFSPDGRMLASGSTDKTIILRDAITRERIGPPLISPGAVLSVAFSP
                     DGKMLAAGSDDNTITLWDVNLYQAIDQPLTGHTAQVGTVAFSPDGRILASAGDDNIII
                     LWDVAASQRIGDPLTGHLGSVHTVAFSPNSRWLASASEDKTILVWDVATRSPVGEPLT
                     GHVGAVHSVAFSPDSQILASGSNDNTIILWDMNTRQPIGRTLGRHSNLVSSLAFSPDG
                     KTLASASYDNSIILWDVQTQQPISRPLKGHTDWILTLAYSPDGTKLASGSADKTVIVW
                     ETSIQQRIDHLLLGDASSVNSVAFNPTGMILASGSHNNTINLWDVTSQLPIGQPLRGH
                     TKPVRSVTLSSDGVLLASGSDDGRVILWNVATQPPIGQPLKGHTGPVYSVAFSPDGSL
                     LASGSADQTLRLWDVRKGEPIGEPLEGHEGPIYSVAVSPDGKVLASGSGDRTIILWDV
                     ATQQLIGQPLKNQSDQIKTVAFSPNGKWLVSGSFNGTIILWNMSTQPPSGQALATDLG
                     FIAEVAFSPDGTMLAAGLYDTTIVLWDVEARQRIGQPLAAHTGSIHGIAFSPDGMTLA
                     SGSVDNNIVLWDINLETWLVKSCQRAGRNLTRVEWEQYFPSEEYRKTCEQWSLEPEES
                     SGSSTAP"
     gene            complement(5190..5927)
                     /locus_tag="VFQ23_11305"
     CDS             complement(5190..5927)
                     /locus_tag="VFQ23_11305"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9907225.1"
                     /translation="MPDYENEVFVSYAWGGEREEIVNQIDKSLQERGIKMIRDKRALG
                     YKGSISQFMERIGQGNCVIVVISDKYLRSPNCMFELVEIAEGKQFHDRIFPIVLGDAD
                     IYDPVKRLGYVKHWEAKRAELAAAMKDVDPANLQGIREDMDLYDRIRDKVSGLTSILK
                     DMNTLTPDMHRDSDFGEIYDGIEKRMKEGPAASTKEASNSEERSKPASNVSASNNSIG
                     IGGISIGGNVSGNFVIGNNNQVNNEEK"
     gene            complement(6002..6496)
                     /locus_tag="VFQ23_11310"
     CDS             complement(6002..6496)
                     /locus_tag="VFQ23_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9907226.1"
                     /translation="MYQELVNSTEMIRASIKDIDQEQAQVRPDAESWSILEVICHLYD
                     EEREDFREHLDFILHRQHEEWHRIDPEGWVTERKYNEQSLVEITDHFFAEREKSLEWL
                     MEISVADMETTYTSAYGSMKAGDMFSSWVAHDSLHVRQLTELRRQYIERITQPYDLQY
                     AGDW"
     gene            complement(6527..7159)
                     /locus_tag="VFQ23_11315"
     CDS             complement(6527..7159)
                     /locus_tag="VFQ23_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET9907227.1"
                     /translation="MNFHGKRVLFLGAHPDDIELGCGALLHHIAKHTDVLCVTLSDNQ
                     ANPELKNVQEEHYESMAVLGVTKEHIIFGPFRTRVFPNARQDILEYFLKLRREFNPDL
                     IFTHSKQDVHQDHNTMTDESLRAFRGITVLGFDVVRSSYGFFPHFLVEVTEEDVNKKM
                     EALSQYETYRDRYYFNSELTRSIMVRHGALAEIPFAEGFDILRIVGKFGE"
BASE COUNT         1694 a         1902 c         1663 g         1936 t
ORIGIN      
        1 aagaatgaac aaaaggcaca tcttatatca ctgtacggta attgtcctgg ctatgaattt
       61 gtcttgtacc actcaatccc ctacgtcttt gcctgtcgaa caacccactg cggtttccac
      121 tgcccttgca tcctcaacgc caccaagtga ggctacccaa gagaatgcct ctgttgctac
      181 cgttacagcg gtgatcgcgg ttacaggtca tttgatgaaa ccggccgaat tggcaccgaa
      241 gcccgggaat ttgatctacg atgtagaatc atcaggaata gctgctccgt atggggatct
      301 gtttaagctt aatcgcctcg aacggccatt ccttgccgat atgacttacg tctctgacat
      361 ggatatcaca tccttcaacc tgagcgaaga ttcagattgg tattatgtct ccattgaatt
      421 gagtgggaat gatcccaaca attccattgg aatcaattac ggcgttgaga tcgatctgga
      481 ctttgacggt tttggcgatt acatcttgtg gtcacagccg ccgtattcaa cttcctggga
      541 aacgacgaat ctgaaggtat ttaaggattc agaccatgac tcagcgggac tctccggcac
      601 tcaatctgat accggtctcg gcggcaatgg atatgatgag ttggtgtttg acggtgaagg
      661 cggtcaaaac gatgaccccg acctggcatg ggtacgtgta aatacagagc cgaacgccgt
      721 cgttcaattt gtattcaaga aatcgttagt aggcagtttc tttatgttgg gcgtagtttc
      781 agatgccggg ctaaaagaca tctccaaatt tgattataac gactcatttg aagaagctga
      841 ggctggttcc cctgataaaa ataaggcgta ttatccctta ggatcgcttt acggggtgga
      901 taatacctgt tgggaagcct acggtattca aacgactggt tatgaaccaa aactatgcca
      961 gcccatttta cagcccacac ctacgtttca accttttcaa cctttgcaac cttcaatacc
     1021 aacagatgaa ggtttcgatt gtattcctga tgtcccgccg gatagctgtg gatttgacga
     1081 agaaactggc gcaccttatt acaaccctgc cacctgcaca tgtacataac tctgtattgc
     1141 aggtgtgcac gaagtagagc ggcataacca tacataaata tcccacaaat gcctatggcg
     1201 ccgtactgga gcctgagctt tcttcaggct ccagagacca ttgctcacag gtctttcgat
     1261 attcctccga cggaaaatat tgctcccatt caacgcgagt cagattgcgg cctgcgcgtt
     1321 gacaggactt gaccagccac gtttctaaat taatatccca caggacaatg ttgttatcta
     1381 cactcccgga tgcaagtgtc atcccatctg gactgaatgc aatgccatga atggagcctg
     1441 tatgcgccgc taagggttga ccgatgcgct ggcgagcctc cacatcccac aaaacgatgg
     1501 ttgtgtcata caaccctgca gcaagcatcg ttccatccgg actaaaggcc acctcagcaa
     1561 taaaccccaa atctgtggcc agagcctgac cggatggcgg ttgtgttgac atattccata
     1621 gaatgattgt gccgttaaaa ctgcccgata ctaaccactt tccatttgga ctgaaggcga
     1681 ctgtttttat ctggtcagat tgattcttga gcggttggcc gatgagttgc tgagttgcca
     1741 catcccacaa gataatcgtc ctgtcgccgc tgcccgatgc gagcaccttt ccatctggac
     1801 tcactgctac gctgtaaatc ggaccctcat gtccttcaag tggctcacca atcggttcac
     1861 ctttgcgaac gtcccataat ctcagtgttt gatcagcact gcccgaagca agcaaactcc
     1921 catccgggct gaatgccaca ctatacactg gccccgtgtg cccttttagc ggttgcccga
     1981 tcggcggctg agtggccaca ttccatagaa ttacacgacc atcatcactg cctgaagcga
     2041 ggagaactcc atccgaactc aaggtcacac ttcttacagg ttttgtatgg cccctgagcg
     2101 gctggccgat tggtaattgt gatgtgacat cccaaaggtt tattgtgttg ttatgactcc
     2161 ccgacgcaag aatcatgcca gtgggattga atgccacact attgacggag ctcgcgtccc
     2221 ccaaaagcag atgatcgatc cgctgctgaa tcgaagtttc ccacactatg acagtcttat
     2281 cggcactgcc cgaggcaagt ttggttccat ccggactata tgcaagcgtc agaatccaat
     2341 ctgtatggcc ttttagtggc cggctgatag gttgctgggt ttgcacatcc cataggataa
     2401 tggaattgtc gtaacttgct gaggcaaggg ttttcccatc cgggctaaac gcaaggctcg
     2461 aaacaagatt cgagtgacgc ccaagagttc gcccaatggg ttgacgggta ttcatatccc
     2521 acaggatgat cgtgttgtca ttgcttcccg aagcaagaat ttggctatcc gggctgaagg
     2581 cgacagaatg gacggcaccc acgtgcccgg tcagaggctc accaactgga ctgcgtgtcg
     2641 ccacatccca caccagaatt gttttgtcct cgctcgccga agcaagccat cggctatttg
     2701 ggctgaatgc gaccgtatga acgctaccta aatgtccggt tagaggatcg ccaattctct
     2761 ggcttgcagc aacatcccat aagatgatga tattgtcatc ccctgcagag gcaagtatcc
     2821 ttccatcggg actgaatgcc acggtcccta cttgagcggt atgtccagta agaggttgat
     2881 caattgcctg ataaaggttc acatcccata aagtgattgt gttgtcatcg ctccccgcgg
     2941 caagcatctt gccatccgga ctgaatgcca cgctcaagac cgcccccgga cttatcagcg
     3001 gcgggccaat ccgttcccgg gtaattgcat ctctcaggat aatggtttta tcagtgcttc
     3061 cggaagcgag catcctccca tccgggctga atgccacgct ggtgacagaa tctgaatgcc
     3121 ccaccaggaa ctccaatagc tgagagttga actgaaggtt ttctaataag acgctgcgcg
     3181 tctggtacgt gttctcattc ttaaaggctt ccatccctaa tagcaaagag agcgccaaat
     3241 tcctctcccg caacgcggtg gattgtgcca cgatttctct cacatgtgct atttgttgct
     3301 gacgttgtgc ttcctcagca tttgactccg ctgtagctct ttgcgagact gcctctgtgc
     3361 ttgccgcttg cgcagtaccc gctattttca gatatctatt cgattgatta ataaacacca
     3421 cgactgcaat cagcaacaaa gcaataatgg tcgatacgcc tcccactacc gctgcctgat
     3481 ttctccgtct ttttacattt ccagcccgaa tgaattcctg tgccatattg cttagcacaa
     3541 gtttttctgt ggcaagctgt tcctgcacat tgaccaatcg cgcgccagaa taaagataac
     3601 ttgggtctcg cttttgctca tcccattcct ttgcatcttc agcaatctgg ttctgcaaag
     3661 cgatcacctc gcgattgtcg ttcaccagtt tctttagcca gggccaggcg tcaatcaatt
     3721 tctcatgcga gatcgtcacc gtatctttgc cggcctgctc atccgtaata attaagcgtg
     3781 catctgccag cttctgcacg atgacttcca caacctgggc gtttgcattc gccgggatga
     3841 gttcatcaaa aagagccgta cgccgtgtgt cttgtgtgcc ccgtccgatt tcgatgagtc
     3901 cagtaaagat ggaccgcgcc agttcttgct cattgagttc taatttcgca aatgcggcat
     3961 cggcatgacg ctctagcgat ttttgtatcc cgccgtgtcg aaggtaatca tcaagcgtga
     4021 gagagatcaa cccccccttg gcgtgctggg aatcaaacaa gtcctttagc gtgaactgca
     4081 tcaacggaag cgcgccgggt tcgcctttca tatcattgat tacctgggcg atcaggtcgg
     4141 ggtcgattcg caagccgaca cgtaaggcag gctgtgccat cgcgctgacc agctcatcgg
     4201 gctgcatagc accgatttga ataaattgtt cattcaggag agcattaagg tcagtatatg
     4261 ttgcgcagtt cgagaggaag tccgagcgca tggcaaacaa gagaatcaca cgtccatttt
     4321 caacagaggc ggtatgcgtg agcaaattga ggaacgcctt gcgctcttcc tctttattga
     4381 tctgtgtgaa tacctcttcg aattggtcaa tgaagatcac agcgcgtttg tctcggctgt
     4441 ctttcaaagc aatctcgcac cagcgcgcga gaatcgttgg atctgcgctt gctttggctc
     4501 ggatttcgtc ttccgcattg gtggaacttg ccaaactgga tgcgacccgt cccaattccc
     4561 cgatcggatc gcgtcccggc ttcattgttt catacatcca gcgctggctg ttcttcagcg
     4621 ctccctcttt caaagcgtga atcaatcccg cccgtaccaa tgaagatttc ccgctacctg
     4681 acggtccggt gacaaacagc gttcgagatt cttttactcg cgttacaagt tcatctacca
     4741 acttctcccg cccaaagaag aactcggcat cctcttcttc aaagacatcc aagcccttat
     4801 atgggcagcg cccatcgaat ggtttgacct gaaactcaga tttgatctga gtgagaagga
     4861 cggagacctg gtcggctgta aatccaatcg tatgaccaac gcgaatatcc ccgacatttc
     4921 caccgacact tattccaccg atggcgatgc tgtggtcgtc tgcctgaact tttgcttcgt
     4981 ccttatcgtt cgcgtcgttc ataccatgcc gtatattaaa atagtctgtc tatcgaacct
     5041 ttatgccact agtgcgccgc tataagtgag cggagtttgg tcttggggat aggcatcggc
     5101 tttggaagtc tatcatgaaa tgaagccatc ataacccgcc tgcgggtctg caacgatcat
     5161 tgattcgtcc ccgcgtttgc tgctggaatt tacttctctt cgttatttac ctggttatta
     5221 tttccgatca caaaattccc actgacattt ccgccgatac taataccgcc aatgccaata
     5281 ctgttattcg aggcggaaac attcgatgcg ggtttggagc gttcctccga attagatgct
     5341 tctttggttg atgcagcagg accttccttc atcctttttt cgataccatc atagatctca
     5401 ccgaaatcgg aatcacggtg catgtcgggt gtgagcgtat tcatatcttt caagatgctt
     5461 gtaagacccg agaccttgtc tctgattcga tcatacaaat ccatatcttc gcgaatgccc
     5521 tgtaaatttg cggggtccac gtctttcatg gctgcggcta attctgccct ttttgcttcc
     5581 caatgtttta catagccgag tcgttttaca ggatcataaa tgtcagcatc acccaaaaca
     5641 atcggaaaga tacgatcgtg aaattgtttg ccttcggcaa tttctacgag ttcgaacatg
     5701 cagttgggag agcgcaggta tttgtcgctg atcacgacga tcacgcagtt accttggccg
     5761 atgcgttcca tgaactgact gatcgagcct ttgtatccca acgcccgctt atcgcggatc
     5821 atcttgattc cgcgttcctg cagtgatttg tcaatctggt ttacgatctc ttcgcgttcc
     5881 ccgccccatg cgtaggacac aaaaacttca ttttcgtaat caggcattag atatctcctc
     5941 gagctcaccg ccaatccgtg cggtgacttg cattatatgt ttcccagacg gcacttgtca
     6001 attaccaatc cccggcatat tgaaggtcat atggctgcgt aatccgctcg atgtactggc
     6061 gcctcagttc cgtgagttgt cgcacgtgca ggctgtcatg tgccacccag cttgagaaca
     6121 tatctcctgc tttcatagaa ccgtatgctg aagtataagt tgtttccatg tctgccacag
     6181 agatttccat cagccattca agagacttct ctcgttcggc gaagaaatga tcggtaattt
     6241 caactaagct ttgctcgtta tattttcttt ctgtcaccca gccttcgggg tcaattcgat
     6301 gccattcctc atgctggcga tgcaggatga agtccaaatg ctcacggaag tcttctcgtt
     6361 cttcatcgta caagtggcag ataacttcca ggatggacca ggattccgca tccggcctga
     6421 cctgagcctg ttcctgatca atgtctttga tcgaagcccg aatcatttcc gtgctgttga
     6481 ccaattcctg atacaaagcc ttgaagtgta tctgatcctc gattctttac tctccaaatt
     6541 tccccacaat gcgcaggatg tcaaatcctt ctgcaaatgg aatttctgcc agcgcgccat
     6601 gccgcaccat gatggagcgc gtcaactcgc tgttgaaata gtatcggtcg cgatacgtct
     6661 catattgcga tagtgcttcc atttttttat ttacatcttc ttcggtgacc tcaaccaaaa
     6721 agtgaggaaa aaaaccgtag gaggatcgaa cgacatcgaa gcccaggacg gtgatgcctc
     6781 ggaacgcgcg cagggactca tcggtcatgg tgttgtggtc ctgatggacg tcctgcttgg
     6841 aatgagtgaa aatcaggtcg ggattaaatt ccctgcggag tttgagaaag tactcgagaa
     6901 tgtcttgcct tgcattcggg aagacgcgcg tccgaaatgg accaaagatg atgtgctctt
     6961 tcgtcacccc aaggactgcc atggattcgt agtgttcttc ctggacgttt ttcaactcag
     7021 ggttagcctg attgtcagag agcgttacac acaagacgtc cgtgtgcttc gcaatgtgat
     7081 gcaacagcgc tccgcatccc agctcgatat catccgggtg cgccccgaga aagaggactc
     7141 gcttaccgtg gaaattcatg ttcaagccct gtcattgcga cgatgagtga agcga
//
LOCUS       DASTJP010000388         7845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1446628, whole genome shotgun sequence.
ACCESSION   DASTJP010000388 DASTJP010000000
VERSION     DASTJP010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446628"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFQ23_11320"
     CDS             <1..568
                     /locus_tag="VFQ23_11320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907228.1"
                     /translation="IFYSPDATFLDYGGGNGMFVRMMRDKGFNYYWYDKFASNQFAAG
                     FEASQEQKFSLITAFEVFEHLLQPLEAVDEMLRYSDAFIFSTRLLPRWKIMPSEWWYF
                     TLDTGQHVSLYSRDSLKVIAQKFNLNFSSNGISLHVLSKIAIPSLLLQALSYPPFSAI
                     LSGLLNLRRPSLLEQDYYRITGVRLDQK"
     gene            565..1740
                     /locus_tag="VFQ23_11325"
     CDS             565..1740
                     /locus_tag="VFQ23_11325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9907229.1"
                     /translation="MRILKVHNFYLQPGGEDTVFKAETALLRDYGHEVIEYVESNQQI
                     ESLSKVSVALGTLWSQPSYQKLKQLLEKLRPDVVHFHNTFPLISPSSYYACQDLGVSV
                     VQTLDNQRLMCPAASFYRDGKLCLDCLGKTPPWPGVVHACYHDSHLHSAIVASMLTLH
                     RWIRTWQTKIDIFLCSTNFYRDLFVRAGLPSQKIVVMPHFVHERNVDISENREGDYAL
                     FVGRLDPEKGINTLLDAWCQLDFPLKIRGSGRMDGDARTFVREHNMDHVEFVGRLEEY
                     ELSNLIRNARFLVMPSEGYYETFGMVIIEAYSRGVPVVASNIGVVPELVVAGQTGLLF
                     ESGDAIDLANKAKWMWQHPSEANVMGRNGRNMYKERFTQDQCYKTLIEVYEGLINSK"
     gene            1737..2480
                     /locus_tag="VFQ23_11330"
     CDS             1737..2480
                     /locus_tag="VFQ23_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET9907230.1"
                     /translation="MTFIVGTRVNSTTYSDATEAVVDWSHRRDSRYVCIANVHMLMEA
                     SDSNEFKRLVNAADLIVPDGMPLVWILRVKGKRSQQRVYGPTLMLYVMEFAARDGIPV
                     GFYGGKPEVLESLTKRMQARYSKLSVVFSCSPPFHVMSPEEDASIVEQINESGARILF
                     VGLGCPKQEIWMAEHRGRVKAVMLGVGAAFDFHAGVKPQAPEWIQKIGLEWLFRFFHE
                     PRRLWKRYLYHNPRFIVLALADIFGLLKN"
     gene            2488..3897
                     /locus_tag="VFQ23_11335"
     CDS             2488..3897
                     /locus_tag="VFQ23_11335"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9907231.1"
                     /translation="MLRRFSVNFALFSMILDGMTVVFCLWFSVAIRPSLSMFSFLKNI
                     DAPISAPYPLYVLFPLIWILIFSSLSIYDGKKFLRAADELAVLMVASIIASVSTAGIL
                     YFSYRDVSRALFVVFIVSTFCVCLLWRALARTYFRTRRESPAISRRLLVVGTGPLGQS
                     VGQQIQNSPTENLKLVGFVDDGYSGENMAPSLGRTEELKTLIEDYQITDVVIALPYSQ
                     YHKMAEITLYLEKMPAGVWIALGFFDLALYRTNIEDFAGIPMLDLRASAIDDYQRLTK
                     RAFDIVFSFIALIIAFPLLALSALLVFLEDGLPILLRQKRVGENGRLFEMLKFRTMVR
                     DAEHLRDQVEKRDRHGNLIHKSKDDPRVTRVGRLLRRLSLDELPQLFNILAGTMSLVG
                     PRPEMPYLVENYQPWQRKRFAVPPGLTGWWQVSGRSDKPMHLHTEDDLYYIQHYSIWL
                     DLQILVRTVWVVLFGKGAY"
     gene            3911..5194
                     /locus_tag="VFQ23_11340"
     CDS             3911..5194
                     /locus_tag="VFQ23_11340"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9907232.1"
                     /translation="MVTRELRFNLTVVIVVLVFFLANLRALMFFALHPELSSIFGFAW
                     IEIGLWFLVAAGVSYSLIRDDLVSRYLSAWRFNWLVGLFVFLALISALWSIAPIVTLY
                     RALELFFAAFVAAYIGIRYSPTQLMEILFWFGAILLILSLGLVFAAPKTGTMYWAPFD
                     GAWRGMYWHRNHLGSITALINMVYLCRAITAIEESDKRGYLDGFFYVFSLVVIYFSES
                     ATGYILILFLHFLVCCIWIWLRVHRHLQSRHYYATLGVFTVGLILIFSNLEFVFGLFN
                     RSTTLTGRVGLWDNLLKYFVPERTWWGYGFGAAWNLDSFREEVRKHVGWTSQPLIADN
                     GFLEILLHLGVVGLLLFVGILLVALIRAFRYAYSQKTLTGFFPLLFLLYILIANIPFS
                     LFAETEVFVWLLIVTTLFMTTSPSEGLMSSDQVAT"
     gene            complement(5200..7743)
                     /gene="mutS"
                     /locus_tag="VFQ23_11345"
     CDS             complement(5200..7743)
                     /gene="mutS"
                     /locus_tag="VFQ23_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9907233.1"
                     /translation="MYNDDVSPARQQYLDIKRDYPNTILFFRLGDFYETFDEDAEITA
                     RELDIVLTSKPVGKGIRAPLAGIPYHAVENYLARLIEKGYHVAICEQMGETPVKGIFP
                     RKVIRVVTPGTVTEPALLPGDSNNYLASLILDEKTASVSYVDITTGEFAVTELPIEAL
                     RAELTRLHPAEILFPDNQTINDGIQSHKTPYPAWRFEPGKCEEILLSHFKASTLQGFG
                     IKGRGLAMRAAGAILQYLRETQPDSLLLLNSLRSYSINEFMTLDAATRRNLELDETLR
                     GERKGSLLGTLDQTVTPMGKRMMHQWVSQPLLNVERIKRRQNGVQAFFDDGMKRTELR
                     AALKPLADLERLVNRVLAGYAQPRDLVAMRDTLARLPKILEIIEHGQISALPKVELAN
                     DELTLLQSSIDEDPPSTLQNTGIIRPGFSQELDSIIEASQHARDWIANLEAVERDRTG
                     LKTLKVGYNKVFGYYIEISRGAADKAPTNYIRKQTLVNAERFITPEMKEYETLVLNAE
                     ERIREIELRLFKETCTILGKSASKLLQTARSLAVLDVLSSLAETAALGGYVRPEVHEG
                     MELEIHEGRHPVVEQLLQGGRYIPNDVVFENGEIVRVITGPNMSGKSTYLRQTALIAL
                     MAQMGSFVPADSAKIGLVDRIFTRIGAQDEIHAGQSTFMVEMVEAANILHHATSRSLL
                     ILDEIGRGTSTYDGLSIAWAVIEYIHNHPHLRAKTLFATHYHELTQLADLLPGIRNYN
                     VAVSEADGKVVFLHKIVSGGADRSYGIHVAQLAGLPSPIIQRASEIMAELEKTSGRAV
                     KIDPHAAQQAMLFPESSPLLDELKELDVNSLSPIEALNKLFEWQRKFIK"
BASE COUNT         1836 a         1738 c         1943 g         2328 t
ORIGIN      
        1 gatattttac agtcccgatg caaccttttt ggactatggg ggcggaaacg gtatgtttgt
       61 cagaatgatg cgtgacaaag gttttaacta ttattggtat gataaatttg cttcaaatca
      121 atttgctgcc ggctttgagg cctctcagga gcaaaagttt tcattgatta ctgcttttga
      181 ggtctttgaa cacctgctgc agccactgga agcagttgat gaaatgcttc gttactctga
      241 cgccttcatt ttttcaactc gattgcttcc gcgttggaag attatgccta gcgagtggtg
      301 gtacttcaca ctcgataccg gacaacatgt ttcactctat tccagagact cattgaaagt
      361 gatcgcccag aaatttaacc taaatttttc atcaaacgga atttccttgc acgtcctgtc
      421 caaaatagcc attccttccc ttctgcttca ggccctaagt tatcctcctt tttcagcgat
      481 actgtctggt ctattgaatt tgagacggcc atctttgctt gaacaagatt attaccgcat
      541 tacgggggta aggttggatc aaaaatgaga atacttaaag tccataattt ttatcttcaa
      601 cccggtggag aagatacagt ttttaaggca gagacggctc tccttaggga ttatggacat
      661 gaagttatcg agtatgtgga atcgaatcaa cagattgaat ccttgagtaa ggtttcggtc
      721 gccctgggaa cactttggtc acaaccctca tatcaaaaac taaagcaact actagaaaaa
      781 cttcgacccg acgtggttca ctttcacaac acattcccac ttatctctcc ctcttcatac
      841 tatgcctgtc aagacttagg tgtttccgtc gtacaaactc ttgataatca aaggttgatg
      901 tgccctgcag cgagctttta tagagatgga aagttatgtt tggactgtct tggcaagacc
      961 cctccgtggc ctggtgttgt acatgcttgt tatcatgatt cccatttaca ttccgcgatt
     1021 gtcgcgtcga tgttgacatt gcatcgttgg atacgaacgt ggcagacaaa gatcgatatc
     1081 tttctatgct ccacgaattt ttatcgtgat cttttcgtgc gtgcgggact tccatctcag
     1141 aagattgttg tgatgccaca ttttgtccac gaacggaatg tggatatatc tgaaaaccgg
     1201 gaaggtgact acgcattatt tgtcggtcgc ctcgatcctg aaaaagggat aaatacactt
     1261 ctggatgcat ggtgccaact ggattttcca ttaaagatac gaggaagcgg acgtatggat
     1321 ggagatgccc gaacattcgt gagagaacat aatatggatc acgtcgaatt tgtaggccgt
     1381 ttggaagaat atgaattgtc gaacctgatt agaaatgccc gctttcttgt catgccatca
     1441 gaaggatatt atgagacatt tggcatggtt atcattgagg catattcaag aggagttccg
     1501 gtggtggcat cgaatattgg agtggtgcca gagttggtcg tcgccggtca aacaggatta
     1561 ctgtttgaat ccggtgacgc cattgacctg gcgaataagg caaaatggat gtggcaacat
     1621 ccttcggaag caaatgtaat gggtagaaat ggacgaaata tgtataaaga gcgttttaca
     1681 caggatcagt gttataaaac tttaatagag gtatatgagg ggttaatcaa ctcaaaatga
     1741 catttattgt tggcacacgt gtgaattcta caacctattc tgatgccacg gaagcagttg
     1801 tcgattggtc gcatcgacgg gatagccgtt atgtctgcat cgcaaatgtt catatgctaa
     1861 tggaagcatc cgattccaat gaatttaaac gactggtaaa cgcggctgat cttatagtgc
     1921 cagatggtat gccgttggtt tggattcttc gagtgaaggg aaaacgtagc cagcagcgag
     1981 tctatggacc aacgctaatg ctgtatgtta tggaattcgc tgcgcgtgat ggtattcctg
     2041 tgggttttta tggaggcaag cctgaagttt tggagtcgct gactaaacgg atgcaggccc
     2101 gttattcgaa gctgagcgtt gtgttttcat gtagtccgcc ttttcatgtg atgagcccag
     2161 aggaggatgc ctctatcgtg gagcagatca acgagtctgg cgcgcggatt ttatttgtgg
     2221 gactggggtg cccaaagcag gaaatttgga tggcagagca tcgcggcagg gtgaaggcag
     2281 taatgcttgg tgtgggcgcg gcgtttgact ttcacgccgg agtcaaaccg caggctcctg
     2341 aatggattca gaagatcggg ttggaatggt tattccgctt cttccatgaa ccacgtcgat
     2401 tgtggaagcg atacctttac cacaacccac gatttatcgt tttggctctc gccgacattt
     2461 tcggactact gaagaattga ttgcactatg ctcaggcgct tttcagtaaa tttcgccctg
     2521 ttttcgatga ttctggatgg catgacggtc gttttttgcc tctggttttc tgttgccatc
     2581 cgaccatccc tgagtatgtt ttcattcttg aaaaatatcg atgcgccgat atcagcaccg
     2641 tatccgttat atgtattgtt tccattgatc tggatcctta ttttttcctc cttatcaatc
     2701 tatgacggta aaaaattttt gcgagcggcg gatgaactgg ccgttcttat ggttgcctct
     2761 atcattgcct ccgtatcgac tgcgggtatt ttatattttt cttatcgcga tgtctcgcgt
     2821 gccttatttg ttgtatttat cgtctctacc ttttgcgttt gcctgctttg gcgcgcgctg
     2881 gcaaggacgt acttccgcac ccgtcgcgag tccccggcaa tctcccgccg cctgctcgtt
     2941 gtgggcacag gtcccctggg gcaaagtgta ggccagcaaa ttcagaattc cccgaccgaa
     3001 aacctgaagc ttgtgggttt tgtggatgat ggctatagtg gcgagaatat ggccccctct
     3061 ctcggcagga ctgaagagct aaaaaccctg attgaagatt accaaatcac agatgtcgtg
     3121 atcgcgcttc cgtactcgca atatcataag atggcagaaa tcacgctgta tctggaaaaa
     3181 atgcccgcgg gcgtttggat tgcccttgga ttttttgatc tggcgttgta tcgcacgaat
     3241 attgaggact tcgcaggaat tcccatgctc gatctgcgcg cttctgccat tgatgattat
     3301 cagcgactaa caaaacgtgc ctttgacatt gtttttagtt tcattgcgtt gatcatcgca
     3361 ttcccacttt tagcgttatc tgctttgctg gtgtttttag aagacgggtt gcctatatta
     3421 ctacgacaga aacgagtggg ggagaacggg cggctgtttg aaatgctgaa atttcgaaca
     3481 atggttcgcg acgccgaaca cttgcgggac caggttgaga agcgagatag acatggaaat
     3541 ctgattcaca aatcaaagga tgaccctcgc gtcacgcgcg tggggcggct gcttcgacgc
     3601 ctcagtctcg atgaacttcc tcaacttttc aatattctcg ccggaactat gagtctggtt
     3661 ggaccgcgcc ccgaaatgcc ctatttggtt gaaaactatc agccgtggca acgaaagcga
     3721 tttgcagttc cacccggttt gaccggatgg tggcaggtga gtgggcggag cgacaagccg
     3781 atgcacctgc acactgagga tgatctgtac tacattcagc attattccat ctggctcgat
     3841 ttacaaattc ttgtgcgaac tgtgtgggta gtgctcttcg ggaaaggtgc atactaagag
     3901 aaaatcaata atggtcacac gagaacttcg attcaatctt actgttgtca ttgtggtttt
     3961 agttttcttc ctggccaatc tgagggcatt aatgtttttt gcattgcatc cggaactatc
     4021 ctcgattttc ggttttgcct ggattgagat tgggctctgg tttctggttg cagctggcgt
     4081 ttcctatagc ctaattcgcg atgatcttgt ctcaagatat ttgtctgcct ggcgattcaa
     4141 ttggctagta ggtctattcg tttttctcgc gttaatctcc gcgctctggt cgattgctcc
     4201 catcgttact ctataccggg cccttgaatt attcttcgca gcttttgtag ctgcctacat
     4261 tggaattcga tacagcccaa cccaactcat ggaaatcctt ttttggtttg gggcaatatt
     4321 gcttattcta tctttgggac tcgtttttgc tgctcctaaa actgggacca tgtactgggc
     4381 tccgtttgat ggcgcgtggc gtggaatgta ttggcatcga aaccatttag gtagtattac
     4441 cgccttgatt aatatggtct atctttgccg cgcaatcacg gcgatagaag aaagtgataa
     4501 gcggggctat ttggatggat tcttttatgt cttttctctg gtcgttatat atttttcaga
     4561 atcagctacc gggtatatcc tgatactctt tctgcatttt ctcgtatgtt gtatatggat
     4621 ttggctcaga gtgcatcgcc atttgcagag tcggcactac tatgcaactc ttggagtctt
     4681 cacagtagga ctgattttga ttttctcaaa tttggagttc gtttttggct tattcaatcg
     4741 aagcaccacg ttaacaggca gagtgggtct atgggataac ttgttgaagt attttgttcc
     4801 tgaacggacg tggtggggat atggatttgg agcggcttgg aatttggatt catttcgcga
     4861 agaggtgaga aaacacgtcg gctggacatc gcaaccctta attgccgata acggattttt
     4921 ggaaatccta ttgcaccttg gagttgttgg attactgcta tttgttggga ttctactggt
     4981 tgcgctgatc cgcgcctttc gatatgcata ttctcaaaag acattgacgg gattttttcc
     5041 tctgttgttt ctgctgtata tattgatcgc aaatatcccg tttagtcttt ttgcagaaac
     5101 tgaagtgttt gtctggcttt tgattgttac tactctgttt atgacaactt ccccctcaga
     5161 aggattaatg agttcggatc aagtagccac ataggcagtt cactttatga acttcctctg
     5221 ccattcgaat agtttattga gcgcctcaat gggagagagg gagtttacat ccaattcctt
     5281 tagttcgtcc agaaggggac tggactctgg aaacagcatg gcctgttgtg cggcgtgagg
     5341 gtcgatcttg actgctcgtc cggacgtttt ctcaagttcc gccatgattt cgctggcgcg
     5401 ttggataata ggagatggca aaccagccag ttgcgcgacg tgtattccgt aggaacggtc
     5461 tgcaccccct gaaacaatct tatgcagaaa gacaactttt ccatcagcct cactgacagc
     5521 tacattgtaa ttgcgaatcc ctggtagcaa gtctgccagc tgggtcaatt catgataatg
     5581 cgttgcaaag agggttttcg cgcggagatg cggatggttg tggatgtatt cgatgacagc
     5641 ccatgcaatc gaaaggccgt cgtaggtgga tgtgccgcga ccgatttcgt ccaggatcaa
     5701 aagcgaacgt gacgtggcgt gatgcaggat attggctgct tctaccatct cgaccatgaa
     5761 cgtggattgc cctgcgtgaa tttcatcctg tgcgccaatg cgggtaaaga ttcgatcaac
     5821 aagtccgatt tttgcggagt ctgccggaac gaacgagccc atctgtgcca tcaaggctat
     5881 cagggctgtc tgacgcaaat aagtggattt gccggacata tttggacccg taattacccg
     5941 cacgatttcg ccgttttcaa aaaccacgtc attcgggata tatcgtccac cctgtaataa
     6001 ctgttccacg accgggtgcc ggccttcatg aatctccaat tccattccct catggacttc
     6061 gggccggacg tagcccccga gggcagctgt ttctgccagg gaggaaagca catcgagcac
     6121 cgccagcgac cgcgcggttt gcagcaattt tgacgctgac tttcctagaa tcgtacaggt
     6181 ttccttgaac agacgaagtt caatttcgcg gatgcgctct tcggcattca acaccagtgt
     6241 ttcatactct ttcatttctg gcgtgatgaa tcgctccgcg ttgaccagag tctgcttgcg
     6301 gatgtaattt gtgggcgctt tatccgccgc gccgcgcgag atttctatgt aatagccgaa
     6361 tactttgttg tacccaactt tcaaagtctt gagcccggtt cgatcccttt cgacggcttc
     6421 aaggttagca atccaatccc gcgcgtgttg tgaagcctcg atgatcgaat caagctcctg
     6481 tgaaaatcct ggccgaatga tgcccgtatt ttgtaacgta gagggagggt cctcatcaat
     6541 agaggattgt aataacgtca attcatcatt tgcaagttcg accttcggta aggcagagat
     6601 ttgcccatgc tcgatgatct ccaggatctt tgggaggcgt gcaagtgtat cccgcattgc
     6661 aaccagatca cgcggctgcg cataaccagc cagcactcga ttgaccaatc tttccaggtc
     6721 cgcaagtggc ttcagggctg cgcgcaattc tgtgcgtttc attccatcgt caaaaaacgc
     6781 ttgtacgccg ttctgccggc gcttgatcct ttctacgttc aataacggtt ggctgaccca
     6841 ttggtgcatc atgcgtttgc ccatgggcgt taccgtttga tccagggtgc caagtaggga
     6901 acccttgcgt tcgccgcgca aggtttcatc taattccaga ttgcggcggg tggcagcatc
     6961 cagggtcata aattcattga tgctatacga tcgcaggctg ttgagtaaaa gcagggaatc
     7021 aggttgtgtt tctcttaaat attgtaggat ggcgcccgct gcgcgcatgg caagtccacg
     7081 acctttgatg ccaaatcctt gcagcgtcga ggctttgaaa tgcgaaagca gaatctcttc
     7141 gcatttgccg ggctcgaacc tccatgccgg gtagggcgtt ttgtgacttt gaattccatc
     7201 attgatggtt tgattatcag gaaacaggat ttctgccggg tgtaaacgcg tcagctccgc
     7261 acgcagtgct tcaatcggta gttctgtcac cgcgaactct ccagtggtga tatcgacata
     7321 ggaaacagaa gcggttttct catccagaat gagtgatgcc agatagttat tcgagtcccc
     7381 aggtagtagc gctggctcag tcacagtccc aggagtcaca acgcgaatta ctttgcgagg
     7441 gaaaattccc ttgaccggcg tttctcccat ctgttcgcaa atggcaacgt gatagccttt
     7501 ttcgatcaat cgtgccagat aattttcaac ggcatgatac ggaatgccgg ctaatggcgc
     7561 gcgaattcct tttccaactg gcttggaggt taatacaatg tcgagttcgc gtgccgtgat
     7621 ttcggcatct tcgtcgaagg tctcataaaa atcgccaagt cgaaaaaaca aaatcgtgtt
     7681 tggatagtcg cgtttgatat caagatattg ttgtcgggcg ggggagacgt cgtcattgta
     7741 catagtaagg ttttattgta ccaagagata attgctggat aaatttaaag tgtgctatta
     7801 tgcttgtagg gtatagaaca attgtactat atttgtaata atcta
//
LOCUS       DASTJP010000389         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_321610, whole genome shotgun sequence.
ACCESSION   DASTJP010000389 DASTJP010000000
VERSION     DASTJP010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321610"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFQ23_11350"
     CDS             <1..542
                     /locus_tag="VFQ23_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9907234.1"
                     /translation="PGQKILIHGASGGVGTFAVQIAKAFEAEVTAVCSTMNLDMVRSL
                     GADHVIDYKREDFTQTSQRYDLILAVNGYRPISSYLRVLNPEGSYVVAGGSMLQLFQA
                     ASARRKNLQNLRKRVSIASLVQNQNDLIFLKELLESGKLVPVIDGCYPINKTAEALWY
                     FEKVHPKGKVIIIVGQNSR"
     gene            complement(1112..1423)
                     /locus_tag="VFQ23_11355"
     CDS             complement(1112..1423)
                     /locus_tag="VFQ23_11355"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9907235.1"
                     /translation="MSTENEMRIKVIVELEARPGKRAELKSLLESVVAKYGPSHPGFL
                     DSTRYEVLDNPNILVEIADWASADVRAAAIQQAMAAGAYAPLEELLAAPFRATVIRQL
                     P"
     gene            complement(1514..2620)
                     /locus_tag="VFQ23_11360"
     CDS             complement(1514..2620)
                     /locus_tag="VFQ23_11360"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HET9907236.1"
                     /translation="MSAPTQHGYLIIADISGYTSFVAKTELEHSQEILSELLELMVSR
                     FQAMMTISKLEGDAVFAYAPTEVFVRSEMLIDFIESMYVAFRDKQLSMKRATTCTCNA
                     CRNIPTLDLKFFLHCGEYIQQTVANIRELVGSDVNLVHRLTKNHVPDATGWRAYMMVT
                     EQCLNHINLNLANAYVQMEQYEHLGEIKTFNVNLHHRYKEITEGRRVILDDKDADFTL
                     QIDFPTPPAVTWEWIHNPDKRNLWNGGHVTWFAGDRPQGRPGIGSSNHCAHGKSVSTE
                     VMVDWHSFEYYTAHSFVKGKHAFTETMRFEALPDGGTRVRDALRMHSPIPRFIRSAAL
                     KHVMITQHHYDKALALAAHLAHDEFTKTRAILTL"
     gene            complement(2643..3077)
                     /locus_tag="VFQ23_11365"
     CDS             complement(2643..3077)
                     /locus_tag="VFQ23_11365"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9907237.1"
                     /translation="MDAIELSIVIRWPVAEAFAFLANLENDVKWHSAFVEVRKTSGGA
                     RFLVFEGVLGRRTPGTEYEVTEYEPNRIVAWKTVSGPLQLKFWRTFERVQSGTRFAVR
                     YEGEPRGFFKLAWPLITRMVKRQQGGDMRKLKELMEVRTLSV"
BASE COUNT          768 a          767 c          773 g          806 t
ORIGIN      
        1 agccagggca aaagatctta attcatggcg cttccggcgg ggtgggaaca tttgcagtcc
       61 agattgccaa agcattcgag gcggaagtaa ctgccgtatg cagcacgatg aatctggata
      121 tggtgcgctc gcttggggcg gatcatgtca tcgattacaa gcgggaagat ttcacccaaa
      181 ctagtcaacg ctatgatctg atcttggctg tgaacggata tcgtccgatt tcaagctatc
      241 tgcgcgtatt aaaccccgaa ggtagttatg ttgtcgctgg aggatcgatg ttgcaactct
      301 tccaggcagc aagcgctaga agaaaaaatc tccagaatct aagaaagagg gtctccatcg
      361 cttcgctagt gcaaaatcaa aatgatttga tctttctgaa agaactcctt gaatccggaa
      421 agcttgtacc ggttatcgat ggatgttatc caataaataa aactgccgaa gccctctggt
      481 actttgagaa ggtacatccc aaggggaaag taattattat cgtgggtcaa aatagcagat
      541 gacgcttcaa tcttctaagc aaagttagat acaacagcat gggaaagaac catcaagagt
      601 tttttgttgg gtccactgtc aaagtctaaa tcttgttgat ctacaaacga tacagtaatc
      661 tcccctgcgg tataatcaaa acatcattca atttactgag cgcccaagta gttacgcgtc
      721 gcgaacccta atcccgtcgg gaccgccaga tgtataacaa aaaatcgacc caaaacgggt
      781 cgattttttg atctaaaaga tatccaaata ctgagcccct tagtagttac gcgttgcgag
      841 gtcgaagaat cggtatgttt tatcgaaccc gcgatccaaa tcgctactca tgtatcctct
      901 aaccttcatg cttgttctaa ttttccaaca agcatcattt gtatgaagtg ctcaaatgag
      961 tgattcaggg atttggagag aaagacaatt ggacgcaagg gggatcatgt tgctgggctt
     1021 gacccgccaa tacgaactgc ggacataaat agaatgcagt gacgtttgga ttctctttta
     1081 taagtaacga tttgaaattt ttttggttac atcaaggcag ttgcctgatg acagtggccc
     1141 tgaacggcgc ggctaacaac tcttccaggg gtgcataagc gcccgcggcc atcgcctgct
     1201 gaatagccgc tgcccgtacg tccgccgacg cccaatcagc aatctccaca aggatgttgg
     1261 ggttatcaag tacttcgtag cgcgtgctgt ctagaaaacc agggtggctc ggaccgtatt
     1321 tcgccacgac gctctcgagc aggctcttga gctcagcccg cttgccgggt ctggcctcga
     1381 gttcgacgat cactttgatt ctcatttcat tttctgttga catacgttcc tcctacgata
     1441 aattggatct gtttcatata tctataatcg tttgctttct acttcttgca ggatgatcga
     1501 caggctcctg cacttatagc gttaggatcg ccctggtttt agtgaattca tcatgggcta
     1561 aatgcgcagc cagcgccagg gctttgtcat agtggtgttg ggtgatcatt acgtgtttca
     1621 gggcggcgct acggatgaaa cgcggaattg gactgtgcat cctgagcgca tcacggacgc
     1681 gtgtgccgcc atccggcaag gcttcaaaac gcatcgtctc cgtaaatgcg tgtttgccct
     1741 tcacaaagga atgcgcggtg taatactcaa aagaatgcca gtccaccata acttccgtac
     1801 tgacggattt gccgtgtgcg cagtgattac tcgagccaat gcctggtcgc ccctggggtc
     1861 ggtcgcctgc gaaccacgtc acgtgaccac cgttccacag gttgcgcttg tccggattgt
     1921 ggatccattc ccaggtcaca gcgggaggcg tgggaaagtc aatttgaaga gtaaaatccg
     1981 catccttgtc atcgaggatg acgcggcgtc cttcggtgat ttccttgtag cgatgatgca
     2041 aattgacatt aaaggttttg atctcgccga gatgttcata ctgctccatc tggacataag
     2101 cattggcaag attcaaattg atatggttca ggcattgttc tgtgaccatc atatacgcgc
     2161 gccagcctgt cgcatctggg acatggttct tggtgaggcg atgaaccagg ttcacatccg
     2221 agccgacgag ttccctgata ttggcaaccg tttgttggat atactcgccg cagtgcagaa
     2281 agaatttcag atcaagggtt gggatgttgc ggcaggcatt gcaggtgcag gtggtcgcgc
     2341 gcttcatgga aagctgttta tcgcgaaacg cgacatacat ggattcgata aaatcgatca
     2401 gcatctcgct gcgtacaaat acttctgtag gtgcatatgc aaagacagcg tcgccctcca
     2461 gtttggagat ggtcatcatg gcttggaagc ggctcaccat tagctcaagc agctcagaga
     2521 ggatctcctg cgaatgttcg agctctgttt tggctacgaa cgatgtgtag ccggagatat
     2581 ctgcgatgat cagataacca tgttgggtag gtgctgacat tttcaactct tgcgtggcga
     2641 tctcatactg acaaagtgcg gacttccatc aattccttca gcttgcgcat atcgcctcct
     2701 tgctgtcgct tgaccatcct ggtgataagc ggccaggcca acttgaagaa gccgcgtggc
     2761 tctccctcat atctgacggc gaatcgtgtg ccactttgga cgcgctcaaa tgtcctccag
     2821 aattttagct ggagaggtcc cgacacggtt ttccaggcca cgatccggtt cggctcgtac
     2881 tcggtaacct cgtattcggt cccaggagtc cgtcggccga gaaccccctc gaacacaaga
     2941 aacctggcgc ccccagatgt tttcctaact tcaacaaacg cactatgcca cttcacgtca
     3001 ttctcaaggt tagccagaaa tgcaaaggcc tccgcaacgg gccacctgat cacgatgctc
     3061 aactcgattg catccatgtt agcccgcttc cagcaattgc tttactgtgt tgag
//
LOCUS       DASTJP010000390         4167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_671376, whole genome shotgun sequence.
ACCESSION   DASTJP010000390 DASTJP010000000
VERSION     DASTJP010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671376"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="VFQ23_11370"
     CDS             complement(<1..1741)
                     /locus_tag="VFQ23_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9907238.1"
                     /translation="MSSFPVAPKRPYEITQHGETRVDDYFWLRYRDDPEVLKYLTAQN
                     DYLDEVMGHTKSLQEKLFAEMKGRIKETDSTVPEKRGEYLYYNRTEAGKQYPIFCRKK
                     DSPEGPEEILLDQNELAEGKIFCSVSAFSVSPDGNKLAYGLDVDGNEVYKIYIKNLAD
                     DTYYPEVIENTFGSVYVRTGIEWANDSENLFYVTLDSAQRPNMLYRHQVGTDLAQDKL
                     IFHEVDNSYYLYFHKSRDDAYILTTHHSTLTTETRFLSADDLDSELKAVSPRKQGIEY
                     FAAYHEGHFCIATNEDARNFKLMKASISNLDREQWQEVVPHREDVMIEWIDTFKNYLV
                     LYERKDGLKQIRISAANGQNDVHYVSFPEEDYYFEIESNPEYDTNILRFKYSSLTTPF
                     SIIDYHMDTREWEQKKEEEIPSGYDKSQFISRRIYATAMDGKLIPISIAHKKDLKLDG
                     NNPTLLHGYGAYGAVLDAEFTPNRLALLERGFIYAIAHIRGGSDMGRDWYDSGKVLQK
                     KNSFTDFIACAEHLIKEGYTSSNKLAIYGVSAGGLLVNASMVMRPDLFKAVIGKVPFV
                     DVINSISDPSIPLT"
     gene            complement(1853..3157)
                     /locus_tag="VFQ23_11375"
     CDS             complement(1853..3157)
                     /locus_tag="VFQ23_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HET9907239.1"
                     /translation="MPNYRVTANVLRIRSGPGTSYEAIGALFRNNIVQGNEINGDWAH
                     VTAADNKVGWCHRGFLELIDESPPPPSQTSYRVDAATLNLRQGPGLNHAVIGSLRRGE
                     IVEGLAVSADNHWAQVRKSGGTTGWASLNYLTRIAASPPPSPTDVEMIVTTDTLNVRS
                     GPGTGYPITGQVHRGETVIYLNASPAWDWVNIKTQENRTGWCSSRYLMERTELFASPE
                     DYQATGLHRALSDSLPMRAGPGENHPIVVDLKFNRVVNVDQLSHDGRWKHCTNAWGES
                     GWYPVERLAKLGTVALQQPTEEFSYIPLAFAEFGTREIRGSQHNPRILEYIRSTDLSR
                     YPSLPDETEWCAAFVNWCIEKVGIPSTNSAVVSPWRNWGVKPPSIRRGCIITFRWDDG
                     WDHISFYLGEVGNYVICLGGNQSDAVWISVYHKKYITSFRIP"
     gene            complement(3288..>4167)
                     /locus_tag="VFQ23_11380"
     CDS             complement(3288..>4167)
                     /locus_tag="VFQ23_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9907240.1"
                     /translation="AVRSLTFHMEAGELLGFIGPNGAGKSTTIKMLTGILHPTGGEAT
                     VLGFVPWRDRQKLAYHIGTVFGQRPQLWYHLPAIDTFTLFGKIFEMEDRDTKLRIGQL
                     AEAFEIKDLLETPVRKLSLGQRMRCEVAASLLHRPRLILLDEPSIGLDVVAKQRIRDA
                     IRDMAQLENVGVLLTSHDAGDLEALCRRVIIVNHGQIVYEDKVSNLKRKFLTSKLVEV
                     RYGEEVPKTFEVSGTELLKTGKHGVKLRFDTRTTPVEKVLSHLTDAGSVVDITISDPP
                     LEEVIAKIYQTQAVVE"
BASE COUNT          978 a         1057 c         1031 g         1101 t
ORIGIN      
        1 tggtgagtgg aatgcttgga tcactgattg aattgattac atccacaaac ggcacttttc
       61 cgatcacggc cttgaacaag tccgggcgca tgaccataga tgcattaacc agcagcccac
      121 cggcagacac gccgtaaatc gcaagcttat tactggatgt atatccctcc ttgatcagat
      181 gctccgcaca ggcgatgaaa tcggtaaaag aattcttctt ttgcagcact ttgccgctgt
      241 cgtaccagtc acgtcccatg tctgaaccac cacgaatatg agcaatggca tagataaagc
      301 cacgctccag caaggcgagg cgattgggcg tgaattcggc gtccaaaacg gcgccatagg
      361 ctccatatcc gtgcaggagt gttggattgt ttccatccag cttaagatct tttttatgag
      421 cgatggaaat ggggatcagc tttccatcca tggcagtcgc gtaaatgcgt cgggagatga
      481 actgcgactt gtcataacca ctggggatct cctcctcctt cttttgctcc cattcgcgcg
      541 tgtccatgtg ataatcaata atcgagaatg gcgtcgtcaa ggaggaatac ttgaaccgca
      601 aaatgttcgt gtcatattcg ggatttgact cgatttcaaa gtaataatct tcttccggga
      661 agctcacgta atgaacatca ttttgcccat tcgctgcact gatgcgaatc tgtttcaacc
      721 catctttccg ttcatagagc accagataat ttttgaatgt atcgatccat tcgatcatca
      781 cgtcttcgcg atgtggaacg acctcctgcc attgctcgcg atctaaattt gagatcgaag
      841 ctttcatcaa tttgaaattt cgcgcgtctt cattggttgc gatgcagaaa tgtccttcgt
      901 gatacgccgc aaagtactca atcccttgtt tgcgcgggga aaccgccttt aactcgctat
      961 cgagatcatc tgctgacaga aacctggtct cggtggtaag cgtgctgtga tgagttgtca
     1021 atatgtaggc atcgtcgcgt gatttgtgaa agtaaagata ataactgttg tcaacttcgt
     1081 gaaagatcaa cttgtcctga gccagatccg tgcccacctg atggcgatac agcatgttgg
     1141 gtcgctgcgc cgaatcgagc gtaacgtaaa acaggttctc actatcgttt gcccactcga
     1201 tccccgtacg gacgtacaca ctgccgaacg tgttctcgat cacttccggg taatatgtgt
     1261 catctgccag gttcttgata tagattttgt agacttcatt gccatctacg tccagtccat
     1321 acgccagttt gttgccgtct ggactcacac tgaacgcgct gacactgcaa aaaattttcc
     1381 cttcggcaag ttcgttttga tccagcagaa tttcctcagg accctccggc gaatctttct
     1441 tgcgacaaaa aataggatat tgctttccgg cttctgtccg attgtaataa agatattccc
     1501 ctctcttctc agggacagtg gaatcggttt ccttgatgcg gcccttcatc tcggcgaata
     1561 atttttcctg caaggacttg gtgtgcccca tgacctcgtc caaatagtca ttttgggcgg
     1621 tcaggtattt cagcacttcc gggtcgtcgc gataacgcag ccagaaataa tcatcgacgc
     1681 gcgtttcgcc atgctgggtg atctcataag gtcgctttgg agcgacaggg aaggatgaca
     1741 tgcttacctc tatactctct attgatgtta tttctctgat tgtttgtaag aatagactga
     1801 tggagttctt tcgttattac tgatattctc gtgtggtcat ggtgtgtcgg cgctagggaa
     1861 tacgaaagct cgttatatat tttttatgat aaacggaaat ccagacggca tcactttgat
     1921 tgccgcccag gcaaatcaca taattaccga cctcgcctaa ataaaacgag atatggtccc
     1981 atccatcatc ccagcgaaac gtgatgatac agcccctccg tatggacggt ggtttcacac
     2041 cccaatttcg ccagggacta acaaccgcag agttggtgga gggaatgcct accttttcaa
     2101 tacaccagtt cacgaacgcc gcacaccatt ctgtttcatc gggcagagat ggatatctcg
     2161 acagatctgt gcttcggatg tattccagaa tcctgggatt atgttgacta ccgcgaattt
     2221 ctcgcgtacc aaactccgcg aaagcgaggg gtatgtagga aaattcctcg gttggttgtt
     2281 gcagggcaac tgtgcctagt ttcgctaatc gttcaaccgg ataccagcca gattctcccc
     2341 aggcatttgt gcagtgtttc cagcgtccat catgtgagag ttgatccaca ttgacgacgc
     2401 ggttgaattt caagtccacg acaatagggt gattctcacc gggtccggct cgcatcggaa
     2461 gcgaatcaga taaagcacga tgaagaccgg ttgcctggta atcttcgggg gatgcgaaca
     2521 gctcggtcct ctccatcaga tagcgtgacg aacaccagcc ggttcggttt tcctgcgttt
     2581 tgatgttcac ccaatcccag gcaggcgagg cgttgagata gattacagtc tcgccgcggt
     2641 ggacttggcc ggtaatggga taccccgtgc ccgggcctga gcgcacattc aaggtatctg
     2701 tcgtgacgat catttctacg tcagtgggac tgggtggggg agacgctgcg attcttgtaa
     2761 gatagttgag tgatgcccag ccagtggttc ctccgctctt acgaacctgt gcccaatggt
     2821 tatcagcaga gaccgcgaga ccttccacta tctcgcctct tcttaaggag ccaatgacag
     2881 cgtgattcaa tccgggtccc tgacggaggt ttaaagtggc tgcatctact cgataggatg
     2941 tttgtgaagg tggcggtgga gactcatcaa tgagttcaag gaaaccccgg tggcaccaac
     3001 ccactttgtt gtctgcggct gttacatgcg cccaatcgcc attgatctca ttgccttgta
     3061 ctatgttatt tctgaacaag gcgcctatcg cttcatagga tgtgccagga ccagagcgaa
     3121 ttcttaatac gtttgcagtg acacggtaat ttggcataac aattccttat aacaactaat
     3181 ccgttggtcg aggagtccat actcttccgc cgtattgaga ccaactctaa tcaaaagaac
     3241 tcttggaact gggtgatttc gatacgtccc gccaagagcg ggcgagacta ctcaaccacc
     3301 gcttgtgtct gataaatctt ggcaatcacc tcttcgagcg gcgggtcaga gatggtgata
     3361 tccacgacgc tccctgcatc ggtgaggtgt gataagactt tctcgaccgg cgtcgtccgc
     3421 gtatcgaagc ggagtttgac tccgtgcttt cctgttttta gtagctccgt tccgctcact
     3481 tcaaacgttt tgggtacttc ttctccgtag cgtacttcga ctagcttcga cgtgaggaac
     3541 ttgcgtttga gattagacac cttatcctca tacacaatct gcccgtgatt gacaataatg
     3601 acgcgccggc acaaggcttc cagatctccg gcgtcatgcg aagtgagaag gacgccgacg
     3661 ttttcaagct gcgccatgtc acggatcgcg tctcggatgc gttgtttggc gaccacgtcc
     3721 aggccaatgg atggctcatc gagcaggatc agcctggggc ggtgaagaag agatgccgca
     3781 acctcacatc gcatccgctg gccaagcgaa agcttgcgta cgggggtttc cagcaaatcc
     3841 ttgatctcga aggcttcagc cagctgacca atccgcagct ttgtatcgcg gtcttccatc
     3901 tcgaaaattt ttccaaagag ggtgaaggtg tcgatggcag gcaggtgata ccaaagctga
     3961 gggcgctgac cgaaaacagt gccgatgtga taggcgagct tttgccggtc tctccaggga
     4021 acgaaaccca ggaccgtagc ttcaccaccc gttgggtgca aaatgccggt gagcattttg
     4081 atggtcgttg atttgcccgc accattcggg ccgataaagc caagtagttc gccagcctcc
     4141 atatgaaagg taaggctgcg aactgct
//
LOCUS       DASTJP010000391         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_217757, whole genome shotgun sequence.
ACCESSION   DASTJP010000391 DASTJP010000000
VERSION     DASTJP010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217757"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VFQ23_11385"
     CDS             <1..1242
                     /locus_tag="VFQ23_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9907241.1"
                     /translation="DAHLTGVVLRLNTDGTAPEDNPFFAAGAEMGDEVGENIQKIFSY
                     GHRNSFGMTVDPTSGGIWLQENGDDTFSELNLVEPGLNGGWIQIMGPAERIEEFKEIE
                     TTFFAPPSTAPALQQLRWPPANLADSPEEAMDRLFMLPGATYGDPEFSWKWEVAPGGM
                     GFVNGKGLGPQFQGDLFVGAATPALEGGYLFHFNLTGNRDQIAVDDPLLEDRVADNLA
                     KHDLTESESLLIGRNFGAVTDIESAPNGNLFLVSLTNGAVYEIFRTSGGGGGGKGEDL
                     EFETETNLTGDQEVPPVMTDMTGNAELVFEGDRMRVRLNIRNNTNDITAAHIHCAPPG
                     ENGPVGVTLFMGSFTDRRGTLVKEFFTAPDAGNECGWASIDDVAQAILDGNAYVNVHT
                     TEESGGFPSGEIRGDIPLPRK"
     gene            1763..2317
                     /locus_tag="VFQ23_11390"
     CDS             1763..2317
                     /locus_tag="VFQ23_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET9907242.1"
                     /translation="MKKIHRTVLIVFVALFATAGIARAAVANYRTHLSGDHEVPPAGV
                     VIETNAQGQAIFQLSPDGSELSYKLIVANIENVFQAHIHIGPPGVNGPIAVWLYPSTT
                     PGAGPLGQGRIQGPIAEGTITSANLVNQGATGITTLAQLIAAIEAGNAYVNVHTNDGV
                     APPNTGPGDFPGGEVRGPLDGHDH"
     gene            complement(2407..2844)
                     /locus_tag="VFQ23_11395"
     CDS             complement(2407..2844)
                     /locus_tag="VFQ23_11395"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HET9907243.1"
                     /translation="MAEEIVIFDIDGTLADVSERLHHIRKKPKDWDAFFRGIPQDKAI
                     HSMVRLCNMLYASGVKILLCTGRREKDRAETVKWLSQQGVNYHELILRPDGDRRSDVI
                     VKREMLAGIDRSKILFVVEDRSGVVEMWRSEGLVCLQCAPGEF"
     gene            complement(2856..3296)
                     /locus_tag="VFQ23_11400"
     CDS             complement(2856..3296)
                     /locus_tag="VFQ23_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907244.1"
                     /translation="MERRFYPAAERFFHRRYIQPAISFLITGFIIEIFYLFRGLQPTG
                     FILGNFLGVWFLNIVIITLTEGFRGEMLAIKVTPDSIYGPIRVGRDMPIPFNEIDFEK
                     TRKMKGIHSIHGDEIRFETALFSSNDVAETWTIIDKFEQKHRNL"
BASE COUNT          897 a          854 c          813 g          874 t
ORIGIN      
        1 gacgcccatt tgacaggtgt tgtgctgcgt ctcaataccg atggcacagc tccagaagat
       61 aatccgtttt tcgccgcggg tgcagagatg ggcgatgaag tcggtgaaaa tatccaaaag
      121 attttctcct atgggcatcg taacagcttt ggcatgaccg tggacccgac ctctggcggc
      181 atctggctcc aggagaacgg cgacgatact ttcagtgaat tgaatcttgt tgagcccgga
      241 ctgaacggcg gctggatcca gatcatggga ccggcggagc gcatcgaaga attcaaggag
      301 atcgaaacaa cgttttttgc acctccttca acagctcccg ccttacagca attacgttgg
      361 ccgcccgcga acctcgcgga ttctccagaa gaggctatgg atcgtttatt catgctccct
      421 ggtgccacat acggagaccc ggaattcagt tggaaatggg aagtagcccc aggtgggatg
      481 ggttttgtga acggcaaggg attaggtccg cagttccagg gtgacctgtt tgtgggcgcc
      541 gctacacctg ctctggaagg aggctatctc ttccatttca atctcactgg caatcgagac
      601 caaatcgctg ttgatgaccc gcttttggaa gaccgtgtgg cagataacct tgcaaaacat
      661 gacctcaccg aaagcgaaag cctgttgatc ggtcgtaact ttggtgccgt caccgatatt
      721 gaatctgccc cgaacggcaa tctgttcctg gtctcgctta ccaacggagc cgtttatgaa
      781 atcttccgca cctcaggtgg aggaggcggt ggtaagggtg aagacctgga atttgaaacc
      841 gagacgaatc tgaccggtga tcaggaagtc cctcctgtta tgacagacat gacaggaaat
      901 gctgagctag tgtttgaagg tgatcggatg agagttcggc tgaacatcag aaacaacacg
      961 aatgacatca ctgcagctca tattcactgc gcgcctccag gtgaaaatgg accggtcggt
     1021 gtaaccctgt tcatgggatc attcacagac cgaagaggta ccctggtgaa ggagttcttc
     1081 acggctcctg atgccggcaa tgagtgcggc tgggccagta ttgatgacgt cgctcaggca
     1141 atcctggatg gaaacgcata tgtcaatgtt catactactg aagaatcagg cggttttcca
     1201 tctggtgaga tccgaggtga tattcccctg cctcgtaagt aactgaaata agcaggcttc
     1261 tgtaacgtca cgatggacgt gatgaccgta gagaagccag atacaaacac caaagtggag
     1321 tcaacgcctg ttgactccac tttttttgat tccatacgcc aggccgtttc gaatatcaat
     1381 actccacaaa aaagagttgg aaatacctaa aatgaaatgg gtaggggttt ccctactctc
     1441 tgaataaatc cgcaaacctc tgaaatccgt aaggattaat aagctaaagg tgtatcgata
     1501 gctcggatat aactttgctt atttttgacg ggtagcggcc gagcttgtca ccttattaaa
     1561 caatactcta tctacacttc aattctttca acgatcgccg agtaccacgt tggctacgat
     1621 caagtcgcct tgacgttccc tttgcagaat tctggtcttg gaaggaggtg atcataattt
     1681 acagcgtgca caagagattt gtccatcttc atcattattg tatccgttcc tgataaattt
     1741 acaaaaagaa aggtgaatac aaatgaaaaa gatccacaga accgttttga tagtcttcgt
     1801 agccctgttc gccacagcgg gaatcgcccg ggcagctgtg gccaattacc gcacccatct
     1861 cagcggagac cacgaagtgc cccccgcggg ggtcgtcatt gagaccaacg cccaggggca
     1921 ggcgatcttc cagctcagcc cggacgggag tgaattatct tacaagctca tcgtcgcgaa
     1981 catcgagaat gtctttcagg cgcatattca tattggacct cctggagtca acgggccgat
     2041 tgcggtgtgg ctctatccaa gcactacgcc cggggcagga ccgcttggcc agggcaggat
     2101 ccagggtccc atagccgaag gaacgatcac ctccgccaat ttggtcaacc agggtgccac
     2161 cggcattacg acactcgcac aattgattgc ggcgattgaa gccgggaatg cctatgtcaa
     2221 cgtgcacacc aatgatggcg tagcgccgcc caacacagga ccgggcgatt ttccgggggg
     2281 tgaggtccgc ggcccgcttg acggccacga ccactaaatt attcccgtag gaatagctat
     2341 agaagtacaa agcccagtgg caaagttgcc gctgggcttc ttcttgataa gaactggttt
     2401 atccggctag aactcacccg gcgcgcactg caaacagaca agaccctccg aacgccacat
     2461 ttcgactacc ccactgcggt cctccacgac gaatagaatc ttgctgcgat caatccctgc
     2521 cagcatctcg cgtttgacga tgacatcgct tcgcctgtca ccgtcaggtc gtaatatcaa
     2581 ttcatggtaa ttgacacctt gctgcgacaa ccatttgacg gtctctgctc gatccttttc
     2641 ccttcgccct gtgcacaaaa ggatttttac tccggatgcg tagagcatat tacataatct
     2701 caccatggag tgaatagcct tgtcctgggg gatgcctcga aagaacgcat cccagtcctt
     2761 tggctttttc ctgatgtgat gcaaacgctc agagacatct gccagagtgc catcgatatc
     2821 gaagattacg atttcctcag ccactgtata ttcccttaca aatttctgtg cttttgctca
     2881 aatttatcga taatggtcca tgtctcagca acatcattgg aactgaataa tgcagtttca
     2941 aatcttattt catctccatg aatgctatgt attcctttca ttttacgcgt cttttcaaaa
     3001 tcaatttcgt taaacggaat gggcatatcc ctgccaactc tgattggacc atatatcgaa
     3061 tcaggtgtta ctttgattgc cagcatttct cctcgaaagc cttctgtaag agtaattatc
     3121 actatattta gaaaccaaac accaagaaaa tttcctaata taaacccggt gggctgaagt
     3181 ccgcgaaata aatagaatat ctcaatgata aaacccgtaa tcagaaagga aattgctggt
     3241 tgaatatatc tgcgatgaaa aaatctttca gccgctgggt aaaatctacg ttccataaat
     3301 tgattatata cttcttctct tcttgataaa aatatacttc attgttgatt tggatgttgt
     3361 agtccacatc aaccatcatt tccaaacttg acgataggtt tgataactat agtattttgg
     3421 tcgggtatcc agcctgat
//
LOCUS       DASTJP010000392        11586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1462462, whole genome shotgun sequence.
ACCESSION   DASTJP010000392 DASTJP010000000
VERSION     DASTJP010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462462"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..380)
                     /locus_tag="VFQ23_11405"
     CDS             complement(75..380)
                     /locus_tag="VFQ23_11405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907245.1"
                     /translation="MEIDLEKIDVAHNEADGQFETWIDGQLSKLDYMLDGNNIVMTHV
                     GVHPAHRGQGVAGKLTQVALEYAKEKSFRVIPMCPYIAAYIRRNTKYLELTKQQVDG"
     gene            complement(482..1378)
                     /locus_tag="VFQ23_11410"
     CDS             complement(482..1378)
                     /locus_tag="VFQ23_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907246.1"
                     /translation="MGFRFRNAVILTLLLTFAAVIPVFAGGWAVITLDELPSDVAAGT
                     PLTIGFTVLQHGKTPMPDLEPTVTAQLSNGKPITFFAAPAEKPGHYTATLTFPEEGNW
                     EWSIQAFTMDQKMPDLNVGAPAAVSVSQPAVPAEAATARSSSPLLVVGILAIGIGVVG
                     LVSAFRYKSRFTLGLTAVCLLIGVGALLAASSAPAKVEAQSSSEAISLSSLSQVELGE
                     QLFVAKGCITCHVNTKVTDYSDYWTIGFEGATNLSNFSAHPDVLRMRLKDPTSVKSDT
                     QMPNLDLTAVEIEALVSFINSK"
     gene            complement(1424..2236)
                     /locus_tag="VFQ23_11415"
     CDS             complement(1424..2236)
                     /locus_tag="VFQ23_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907247.1"
                     /translation="MSELERELFEDQLRSIAKGLDYPRTPDIAGSVTTRLRASTRPRF
                     VSRKLAWSLTMIVVLLSSLMLIPPVRAAVVEFIQIGIVRIFPRPTESPIDSIRTATPD
                     SIRPETATPAIPSAPLLLSMSRIAGETTLANAQETVGYSILLPSYPSELGLPDHVFLQ
                     DAEGVMTILVWLDPADPDRVLMSLHIIPEGSWGFEKSEPRTIEETDVNGQRAIWAVGP
                     YPLRLVNGNLDFTRLIDGNVLIWTDGELTYRLETNQTLEEAIKTAESLEPIP"
     gene            complement(2233..2793)
                     /locus_tag="VFQ23_11420"
     CDS             complement(2233..2793)
                     /locus_tag="VFQ23_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9907248.1"
                     /translation="MDESTLIRHAANGDASAWEPLVLAHQEAVFRLAYLLLGDPDDAE
                     DTAQETFLRAWNYLKRFDQTRPLRPWLLSIAANLARNRRRSAGRYLAALTRAFRNEPL
                     PASVEEKSTKTMEANDLWKAVQNLSLPDQQIVYLRYFLDLSVSETAEVLQIAEGTVKS
                     RLSRALEKLRAIIKQDFPVFSEGNES"
     gene            3121..4458
                     /gene="ablA"
                     /locus_tag="VFQ23_11425"
     CDS             3121..4458
                     /gene="ablA"
                     /locus_tag="VFQ23_11425"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HET9907249.1"
                     /translation="MSTPFVSKRAPVYADVPDEKWNDWRWQLSHRLNNVEEIEKILPL
                     NESERKALQTQGLFRVDITPYFISLIDPDDPDDPIRKQVIPLSEEMQSFTAMMEDSLA
                     EDRHSPVPGLVHRYPDRVLMLVTTQCASYCRYCTRARIVGDPSQTFSRQEFEMQIEYL
                     KRTPQVRDVLLSGGDPLVLAPKILEEILSRLRAIPHIEIVRLGTRVPVFMPMRVTQDL
                     CDMLAKYHPLWVNIHVNHSNEISLELEQACDRLSRAGIPLGNQSVLLAGVNDNVHIQR
                     KLVQDLVRIRVRPYYLYQCDLVEGAGHFRTPVAKGIEIMEGLRGHTSGYAVPQYIVDA
                     PGGGGKIPVMPNYLLSMSDHKIILRNYEGYVASYEEPTDYMPSEAAKFKGERRSEPGQ
                     TGVHGLLEGDQMFIKPEGFDETHDRHGIQHRLKDDKKWQPLGIGDGSTKSNEE"
     gene            4478..5113
                     /locus_tag="VFQ23_11430"
     CDS             4478..5113
                     /locus_tag="VFQ23_11430"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9907250.1"
                     /translation="MNRSTKRILFSVTAVIFLLAACVPAAQPTVDPNEVVNQVATSVA
                     LTVAAQNTQAALERPSDTPVPPPTETAIPSPTLVPDIPTATPFVVVPPTTVAGGGGGG
                     SPVQQEYDCDIIRRRPFDNTFFRPGDEFDIRWTIVNTGTRNLRAGTDVKFSIGSQMVG
                     VTMVELPEMRPGDQYEIIFDAVAPETEGNHIMTWVVEGPLCYPYTAIIVEK"
     gene            5195..5851
                     /locus_tag="VFQ23_11435"
     CDS             5195..5851
                     /locus_tag="VFQ23_11435"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9907251.1"
                     /translation="MLTYRAKVILWLVVVGTVLACVPSLATPLPPVDANAVNTFIAQT
                     VNAASTQTSAALPTSTQTATYTPTPRNTDTPTPTSTATVIFILSTPTSVVIPTFTVVS
                     SGGGSSSDNFACQVLSVSPANGTTFGSRGDFDAVWRVRNNGQRAWDRSEIDYRYTSGD
                     KIHKVEVYDLSGNVPRNGTTDIVVDMVAPRNAGTYSTTWNLYRGARSFCTMTLRIVVQ
                     "
     gene            complement(5928..6212)
                     /locus_tag="VFQ23_11440"
     CDS             complement(5928..6212)
                     /locus_tag="VFQ23_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907252.1"
                     /translation="MDKRQTGLIATITTALLCGCPGLLAFCWGSLSAVISFVPGADIN
                     IGGSSDPSSALYAGLGSLCAGILFIAIPVAVGYVTLRKRPDSNVGLPPEA"
     gene            complement(6279..8879)
                     /locus_tag="VFQ23_11445"
     CDS             complement(6279..8879)
                     /locus_tag="VFQ23_11445"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET9907253.1"
                     /translation="MKIPLSWLKDYIDLDGLTVEEIARKLTLAGLEVDEIKYAGLEMP
                     AYKDGTPHEFKTTGISWDKDKLVVAEIREVMPHPNADRLTLLDLFDGKQQQTVLTGAP
                     NIFHLKGTGKLAKPIKAAYAKEGTTLYDGHADGLQLTTLKRTKIRGVESYSMVCSEKE
                     LGISEEHEGIILLEEDAPVGMALADYMGDAVLDISILPNMARNASVIGVARELAALTG
                     RQLKKPTITLKTEGELVENYVSIEITEPELNPRFVLGLIREVEIKPSPYQIQRRLRLA
                     GMRPINNIVDATNYAMLDLGQPLHAFDYDVLKHRAGKDNVKIITRSAKDGEKLTTLDG
                     NERKLTSVNVLVCDQKGPLALAGVMGGAESEVYDASDEVLDAQGMELTSGGMQQGKVS
                     VRGQSTTTILLEGAAWNFINIRRTAKQHNLPSEASFRFSRGVHPALAEQGVMRGLQLM
                     AEWSGGKIAPGFVDEYPLKPTDPTVTVTPKDVKRLLGIDLTAKQIAELLTRLEFECTV
                     EKNSVKAKTPPHRVDIGEGVVGVADVLEEVARIYGYDNIPETRMADALPPQIGNPVHE
                     WEEHLRDTLVSLGLQEVVSYRMTSPEREGRLESFSKYVAIANPITPERSVLRRSLLAS
                     VLEAAEKNSRAESIAMYEIGSIFEPGQDALPNEPRKLALVMTGQRMATAWDVKDSPVY
                     DFFDMKGRLELLLSGLRYSNVTYNPIDSIPYLHPGKAADVSINGQKVGVFGELHPLVK
                     EKYELGEAPVIVAEFDLDTLRSATPKYGIKPVSEFPPVYEDIAVIVDESVPAGRVESL
                     IRQTGGKTVTDVHLFDVYRGEQIGAGKKSLAYSLTYQSDKTMTDAEAASIRTKIVKRL
                     EHEISAKLRS"
     gene            complement(8888..9946)
                     /gene="pheS"
                     /locus_tag="VFQ23_11450"
     CDS             complement(8888..9946)
                     /gene="pheS"
                     /locus_tag="VFQ23_11450"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET9907254.1"
                     /translation="MLEKLNEIEKSALASLDAVKDPDALEAWRIANVGRSSPLMQVFS
                     ELGKLSKEERPTVGAAANRVKVALESALAERAELVKAAALAKSLEEDALDVTLPGRDI
                     HLGRLHPSTQQLRRVLDILADMGFQVYTSREVETDEVNFQLLNFPPNHPARDMQDTFF
                     VEAEGRGDNPVLLRTHTSPGQIHAMKEYAAMNPENPPPIRIALPGMCFRYEQITARSE
                     IQFNQVEGLVVGEHITLSDLKGTLIDFARRMFGQNVRTRFRASYFPFTEPSAEMDVEC
                     FVCGGKGCQVCKNSGWLEILGCGMVHPIVLQNGGYDPTRYSGFAWGMGPERQLMLRYK
                     IDDIRYFWGNDLRFLEQF"
     gene            complement(10369..11145)
                     /locus_tag="VFQ23_11455"
     CDS             complement(10369..11145)
                     /locus_tag="VFQ23_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907255.1"
                     /translation="MPSFKQTFWKIWYQYLSGRIGSDPVTFLNYGYWPPEGETIELKP
                     QDEPNRPAIQLYHHVASGWDLNDKDVLEVSCGHGGGASFVKCYHQPNTYTAIDQNPKA
                     IEYDRKTHGALGIDFRVGDAQALDFPEGNFDAVINVEASHCYPRQDAFFKSVYRVLRK
                     GGRLLYADFRTPATADQLDRDITNAGFNIESKTDITSFVLSALKRTSQRYRDLVHGLT
                     PAILHPLMETFAAVEGSGVYNSFVNRERIYYSYRLVKAEN"
     gene            complement(11145..>11586)
                     /locus_tag="VFQ23_11460"
     CDS             complement(11145..>11586)
                     /locus_tag="VFQ23_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9907256.1"
                     /translation="IVAGDKVIIEWLRQRGRPFLFSSAVTAPDAAACLAAVDLLEEST
                     ELVDKLWANAKYFKEEMKKLGFDTGVSETPITPVMLGEAPLAQQFSRELFDEGVFAMA
                     IGFPTVAKGKARIRVMISAAHDNDDLGQGLDAFAKVGKKLGVIS"
BASE COUNT         2812 a         3063 c         2845 g         2866 t
ORIGIN      
        1 gatcatcatc ggcaagcaaa cgaacgatgg atggcaaatc atctctcgtg gcaatccgga
       61 agatgattag tttctcaccc atctacttgt tgcttggtaa gctccagata ttttgtgttt
      121 cggcggatat aagcagcaat gtagggacac atcggaatca cccgaaatga tttttccttt
      181 gcatattcca gtgcaacctg agtcagctta cccgcgacgc cctgtccgcg atgcgcgggg
      241 tgaacaccca cgtgcgtcat cacaatatta ttgccatcca gcatgtaatc caatttagac
      301 agctgaccgt cgatccatgt ttcaaactgg ccatcagctt cgttgtgagc tacgtcaatc
      361 ttttccaggt caatttccat aagattatcc ttgtcgattt acaaacaatt gtatcttata
      421 tcccttaaat caaaaacgga cggtatgatg tctcaccgtc cgttcgataa tcaggtggct
      481 attattttga gttgataaac gacaccaggg cttcgatctc taccgcggtt aagtccaggt
      541 tgggcatttg ggtgtcagac ttaacggacg tggggtcttt caatctcatt cgcaatacat
      601 ccgggtgggc tgagaaattg gagagatttg tagcgccttc aaatccgatg gtccaataat
      661 cactgtagtc tgtcactttg gtgttgacat gacacgtaat acaacctttg gcaacaaata
      721 actgttcgcc cagctcaact tgtgaaagcg acgatagact gatcgcttct gaggaggact
      781 gcgcctctac tttagccggt gcactggatg ccgcgagcaa agccccaact ccaattaaca
      841 gacacactgc ggtcaatcct aaagtaaatc tactcttata gcgaaatgct gatacaaggc
      901 caaccacacc tatcccaatt gccagaatac caacgaccag cagcggtgag ctgcttcgag
      961 ccgtggcagc ctcagccgga accgcgggtt ggcttacgga tacggccgcc ggcgcaccaa
     1021 cattgaggtc gggcatcttc tgatccattg taaatgcctg aatggaccac tcccagttcc
     1081 cttcctcagg gaaggttaga gtagcagtgt agtgaccagg tttctcagcg ggtgcagcga
     1141 agaaagtgat tggttttccg ttggatagtt gcgcagtgac agttggctca aggtcgggca
     1201 tcggcgtttt accatgttgc aggactgtaa acccgatggt taggggtgtg ccggctgcaa
     1261 cgtctgatgg cagttcatcc aatgtaatga ctgcccatcc cccggcaaat acaggaatga
     1321 cagccgcaaa cgtcaacagc agagtcaaaa tgactgcatt tcgaaatctg aatcccattt
     1381 gtgcctcctt tggtgaatgt aaattcaata ggatacacct ctcctacggg atgggttcca
     1441 gtgattcggc agttttgatc gcttcttcga gagtctggtt cgtttcgagt cggtatgtga
     1501 gttcaccatc agtccagatt aatacatttc catcaataag acgtgtgaaa tccaaattac
     1561 catttacgag tcggagcggg taaggtccaa ctgcccaaat cgcgcgctgt ccgttgacgt
     1621 ccgtttcttc aatggttctt ggctcagatt tctcaaatcc ccagctccct tccgggatga
     1681 tgtggaggct catcaggact cggtcaggat cggcagggtc gagccagacc aggattgtca
     1741 ttacgccttc tgcatcctga aggaaaacat gatcaggcaa acctaattca gatggatacg
     1801 aaggcagaag aattgagtag cctactgtct cttgagcgtt cgcaagcgta gtctcgccag
     1861 caatgcgact cattgagaga agcaaaggcg cagacggaat tgcgggcgtg gcggtttctg
     1921 gtctgatgga gtcgggtgtc gcggtgcgga tgctatcaat cggtgactcg gttgggcggg
     1981 ggaagattcg tacgataccg atttgaataa actcgaccac cgcggcgcgt accggcggaa
     2041 tcagcatgag gcttgagagg aggaccacga tcatggtcag tgaccaggcc agtttccggg
     2101 aaacgaaacg cggacgagtc gaagcacgca gacgcgttgt tacagatccg gcaatatccg
     2161 gcgttcgagg atagtccaat cctttggcga tggatctcaa ttgatcttca aagagttcgc
     2221 gttccaactc gctcatgact catttccttc ggagaaaaca gggaaatcct gcttaataat
     2281 tgctcgtaat ttttcaagcg cacggctgag ccgtgatttg acggtaccct ccgcgatttg
     2341 tagcacttcg gctgtttcac taaccgataa atccaaaaaa tagcgcagat aaacgatttg
     2401 ctggtctggc aggctcaggt tttgaaccgc cttccacaaa tcattggctt ccatggtttt
     2461 cgtgcttttc tcttccacgc tcgccgggag cggctcgttg cgaaatgcac gcgtcaacgc
     2521 cgcaaggtaa cgccctgcgg aacgacgccg attgcgagcc agattcgcag caatgctcaa
     2581 tagccacggc cgcaacgggc gcgtttgatc aaagcgcttg aggtaattcc aggcgcgtaa
     2641 aaaggtttcc tgggcggtat cttctgcatc atcgggatcg ccaaggagta gataagccag
     2701 ccggaaaacc gcctcctggt gcgccagcac caacggctcc caagccgacg cgtcgccgtt
     2761 tgcggcgtgt cgaattagag tggactcatc caatcatgcc tctccggaga ggacctgttt
     2821 tcataggtta tacaccgtta aatgaaaccg ggttccactt ttcttattat tcacccggtt
     2881 cttggaaccg aatttcggtg aggaaacaat atattacata aacgaatcct ggattcataa
     2941 ccagtccaaa gggacattga cattgaccct atttttgttt gctcttacaa ttcaaatcat
     3001 gataaacgcg tgtatctcta atgctggctt ttatcggcca gatcatgatc ctccgtgcca
     3061 tgcattttgc gcgtcaattt atcgaatcaa ctaactaaaa caatctcgga ggaaaatttt
     3121 atgagtacac cttttgtttc aaaacgtgcc cccgtttatg cggatgtacc tgatgaaaag
     3181 tggaacgact ggcgctggca attaagccat cgtttgaaca atgtagaaga gatcgaaaaa
     3241 atcctgccgc taaatgagtc tgagcgaaaa gcgctccaga cccagggcct gtttcgtgtg
     3301 gacatcacgc cgtactttat ttcgctgatt gatccggatg atccggatga cccgattcgg
     3361 aaacaggtca ttcccctttc ggaagagatg caatctttca ccgcaatgat ggaagactcg
     3421 cttgcagaag acagacactc cccagttccg ggtcttgttc atagatatcc agacagggtg
     3481 ctgatgctgg tcacgactca atgtgcgtcg tattgtcgct attgcacgcg ggcgcgcatc
     3541 gtcggtgacc ccagccaaac gttctcacgc caggaatttg agatgcagat cgaatatctc
     3601 aagcggactc cacaagtccg tgatgtgctt ctctccggcg gcgaccccct ggtactggcg
     3661 ccaaaaatat tggaagaaat cctcagccgc ttacgcgcaa tccctcatat tgaaattgtt
     3721 cgcctgggga cgcgcgtgcc cgtcttcatg ccgatgcgcg tgacccagga tttgtgtgat
     3781 atgcttgcga agtaccatcc cctgtgggtc aatatacacg tgaaccattc caatgaaatt
     3841 tcgctggaac tagaacaagc ctgcgaccgt cttagtagag caggtattcc gcttggaaac
     3901 cagtctgtgt tacttgctgg tgtgaacgat aacgtccata ttcagcgcaa attagtgcag
     3961 gatctggtcc gaatccgagt tcgtccctac tatctctatc aatgtgacct ggtggaaggt
     4021 gcggggcact tccgcactcc ggtcgcgaaa gggatcgaaa tcatggaagg tctgcgcggc
     4081 cacacctcgg gttatgctgt cccacaatac attgtggatg ccccgggtgg aggcggcaag
     4141 attcccgtca tgccaaatta tttgttgagc atgtctgatc acaaaatcat cttgcgtaat
     4201 tacgaaggct acgttgcctc ttacgaggaa cccaccgatt acatgcccag tgaagcagca
     4261 aagttcaaag gcgagaggcg ttccgaaccc ggtcagaccg gcgttcacgg attgctggaa
     4321 ggcgatcaga tgttcattaa acctgaaggc ttcgatgaaa cacatgatcg tcacggcatt
     4381 caacatcgcc tgaaggatga taagaaatgg cagcccctgg gtatcggtga tggctctacg
     4441 aaatcaaacg aggagtaagt aaacgaagga gatatgcatg aaccgatcta caaaacgaat
     4501 ccttttctct gtgacggctg tgatcttttt gttggccgcc tgtgtgccag ctgcacaacc
     4561 caccgtagat ccaaatgaag ttgtgaacca ggttgcgacc tcggtggccc tgaccgtcgc
     4621 tgcccaaaat actcaggctg cactggagcg tccttccgac acacccgttc cgcctccaac
     4681 ggaaacggcc attccttccc caactctagt ccctgatatt ccgacagcaa caccgtttgt
     4741 ggttgtacct ccaacgactg tggcgggtgg tggcggtggc gggtcgcctg tccaacaaga
     4801 gtatgactgt gacattatcc gccgccggcc attcgacaat accttcttcc gccccggcga
     4861 tgaatttgac atccggtgga caattgtcaa cacgggcacc agaaacctgc gggccggcac
     4921 agatgtaaaa ttttcaattg gttctcaaat ggtcggagta acgatggttg aacttccgga
     4981 aatgagaccc ggtgaccaat atgaaatcat tttcgatgca gttgcaccgg agacggaagg
     5041 caaccacatc atgacctggg ttgtagaagg tccattgtgt tatccgtata cagcgatcat
     5101 tgtggagaaa tgatagaacg tatctggaag aatacgctga gctagctatc gttgcgagaa
     5161 gtgtttctct ggcttcggat gtgcaagagg aaatatgtta acttatagag caaaagtaat
     5221 tttatggctt gtcgtggttg ggaccgtatt ggcttgtgtc ccttcgcttg cgaccccatt
     5281 gccacccgta gatgccaacg cagtcaacac attcatcgcg caaacggtca acgctgcatc
     5341 gactcaaaca tcggcagcct taccgacttc cacgcagact gcaacctata ctcctacccc
     5401 gcgcaatacg gatacgccaa caccgacttc cacagcaaca gtaatattca tcctctccac
     5461 tccaacgtct gtggttattc caacgtttac cgttgtgagt tcaggtggag gcagcagttc
     5521 cgataatttt gcgtgtcagg tgctgagtgt ttcaccggcc aatggtacaa catttggttc
     5581 gcgtggagat ttcgatgccg tctggcgtgt gagaaacaac ggccagagag catgggaccg
     5641 ctctgagatc gattaccgtt acacaagtgg agataagatc cataaagtgg aagtctatga
     5701 cctgtctgga aacgtcccca gaaatggaac aaccgacatc gttgtagata tggttgcacc
     5761 ccgcaatgct ggcacctact caacaacgtg gaatctgtat cgcggtgcca gatccttctg
     5821 caccatgacc ctgcgtatcg tggttcagta aattcaaaag taatccatcc aataaaagaa
     5881 agagcggcga cctggacagg tcgccgctgt gttttgaacg tgtagtccta ggcctcgggt
     5941 ggcaggccca cgtttgaatc tggtcttttg cgcaaggtaa cataaccaac cgcaactgga
     6001 atggcgataa acaaaatgcc agcacataat gagcccaagc ctgcatacaa cgcagagctt
     6061 gggtcgctgc tgccgccgat attgatatct gcgccgggca caaaactgat aaccgccgaa
     6121 agacttcccc agcagaatgc cagcaacccc gggcacccac atagtaacgc cgtagtaatc
     6181 gtggcaatta agccggtctg tcgtttatcc atatgaattc tcctttgagt agatatgact
     6241 gagtagattt tggtccatat aatacaagcg caagtagatt aactccgtaa ctttgcgctg
     6301 atttcatgtt ccagacgctt gacaatcttg gtccggatcg aggcggcttc ggcgtcagtc
     6361 atggtcttgt ctgattggta ggtcagacta tacgccagcg acttcttgcc cgcgccgatc
     6421 tgttcgccgc gatagacatc aaacaaatgg acatcagtga cagtctttcc acctgtttgc
     6481 ctgatgaggc tctcgacccg ccccgccggc accgactcat caacgatcac tgcgatatct
     6541 tcatagacgg gcggaaattc tgataccggt ttgattccat acttgggggt agcgctccgt
     6601 aatgtgtcca ggtcaaactc agcaacaatc acgggcgcct caccgagctc gtatttttcc
     6661 ttcaccagtg gatgcaattc tccgaagaca ccaactttct gtccgttgat tgatacgtct
     6721 gcagctttgc caggatgcag ataaggaatg gaatcgattg gattgtaagt aacgttcgaa
     6781 taacggagac cactcaaaag gagttcgagc cgtcccttca tatcgaagaa atcatagact
     6841 ggcgaatctt taacatccca ggcggtcgcc atacgttgac cagtcatcac cagcgccagt
     6901 ttgcgtggtt cattgggtaa agcatcctga cccggctcaa aaatcgagcc gatctcatac
     6961 atggcaattg actcggcgcg cgaattcttc tctgccgctt caagcaccga tgccagcagg
     7021 ctgcgacgaa gaacacttcg ctcgggcgtg atggggtttg caatcgcgac atacttactg
     7081 aatgactcaa gcctgccttc gcgctcaggc gacgtcatgc gatagctgac tacctcctgc
     7141 aaaccaaggc taacgagagt atcgcgtaag tgctcttccc attcatgcac ggggttacca
     7201 atttgcggtg gcaatgcatc tgccatgcgc gtttcaggga tgttgtcata tccatagatg
     7261 cgggcaactt cttcgagcac atctgcaacg cccacgactc cttcgccaat atcaacccga
     7321 tgtggcggcg ttttcgcttt gacggaattc ttctcaacgg tacattcgaa ttcaagccgc
     7381 gtcagcagtt ccgcgatctg tttcgctgtg agatcgatgc ccagcaatcg cttcacatcc
     7441 ttcggagtga cagtaaccgt tgggtctgtg ggtttgagcg gatattcatc cacaaatccg
     7501 ggagcgattt tgccccccga ccattccgcc atgagttgaa gcccgcgcat tacgccttgc
     7561 tcggccagcg cggggtgaac accgcgcgag aatcggaagg aggcttcaga aggaagattg
     7621 tgctgtttcg cagttcgacg aatattgatg aaattccacg ccgctccttc cagcaggatc
     7681 gtggtggtgc tctggcctcg aacactcacc ttgccctgct gcataccacc agaggtcagc
     7741 tccatgcctt gtgcatcgag aacttcatcg gaggcatcat agacttcact ttcagcgcca
     7801 cccattacgc cagcaagcgc cagaggtcct ttctgatcgc agaccaaaac gtttacagag
     7861 gtgagcttcc gttcgttgcc atccagcgtt gttagttttt cgccgtcttt tgccgaacgt
     7921 gtaattattt ttacgttatc tttgccagcg cgatgtttta gcacatcgta atcaaacgcg
     7981 tgaagcggtt gaccgaggtc cagcatcgcg tagttcgtcg cgtccacgat gttgttgatc
     8041 gggcgcattc ctgccaacct taaacgccgc tgaatttgat aagggcttgg tttgatctca
     8101 acctcgcgta tcaagccaag cacaaaacgc ggattgagtt ccggttcggt aatttcaatg
     8161 gaaacgtaat tctcaaccag ctcaccttca gttttgagtg tgatggttgg ctttttgagt
     8221 tgacgtcccg tcaaggcggc aagctcgcgc gccacaccaa tgacgctcgc attgcgcgcc
     8281 atattcggaa ggatgctgat atcgagcaca gcgtcgccca tgtaatctgc cagcgccatt
     8341 ccaaccggag catcctcttc gagcagaata atgccctcat gttcctcaga gatgcccagt
     8401 tccttctctg agcagaccat tgaatatgac tcaacgccac ggatttttgt ccgtttgagc
     8461 gtcgtcaact gcaagccatc cgcatgccca tcgtatagcg tcgtaccttc tttggcatac
     8521 gcggctttga tgggtttggc tagtttgccc gtgcctttca agtgaaagat atttggcgca
     8581 ccggtcagaa cagtctgttg ttgcttgccg tcgaatagat ccagcaacgt taatcgatcc
     8641 gcgttgggat ggggcatcac ctcgcgaatc tctgcaacca ccagcttgtc tttatcccag
     8701 gaaatgcctg tcgttttgaa ttcgtgaggt gtgccatctt tatatgcagg catttccagc
     8761 ccggcgtatt taatctcgtc aacttcgagc ccggccaggg taagtttgcg ggcgatttct
     8821 tcaaccgtca aaccatcaag gtcaatataa tctttgagcc atgaaagagg tattttcact
     8881 ctgcctccta gaattgttca aggaaacgca aatcgtttcc ccagaaatag cgaatatcgt
     8941 caatcttata gcgcagcatc aactgacgtt caggtcccat gccccaggcg aagcctgagt
     9001 agcgcgtggg atcatatccg ccgttttgca aaacaatcgg atgcaccatg ccacacccaa
     9061 ggatttcaag ccagcctgaa tttttacaga cctggcaacc cttgccacca cagacaaaac
     9121 actccacgtc catctctgca gatggttctg tgaacgggaa gtacgaggct cggaaacgtg
     9181 tccgcacgtt ttgaccgaac atgcggcggg cgaaatcgat cagtgtccct ttcaagtcag
     9241 acagtgtgat gtgctcacct accacgagcc cttccacttg attgaactga atctctgaac
     9301 gagctgtgat ctgctcgtaa cggaagcaca ttccaggcag cgcaatgcga atcggtggag
     9361 gattctcggg gttcatcgcg gcatactctt tcatcgcatg gatctgcccc ggcgaggtgt
     9421 gtgtacgaag taaaaccgga ttgtcaccgc gtccctccgc ttcgacgaag aaggtatcct
     9481 gcatatctcg cgccggatga ttgggcggga agtttaataa ctggaagttg acttcatcag
     9541 tctcaacctc gcgcgacgta tacacttgaa aacccatgtc cgcgagaata tccagcacgc
     9601 gccgcagctg ctgcgtggat gggtgcaagc gccccaggtg gatatcccgc ccgggaagtg
     9661 tcacatccag ggcatcttcc tcaagggatt ttgccaaagc ggcggctttg accaactcag
     9721 cgcgttccgc taaagccgat tcaagcgcta ccttcacgcg gttcgcggcc gcgccgaccg
     9781 ttggtcgctc ttcctttgag agttttccca gttccgaaaa gacctgcatc aggggcgagc
     9841 tccgcccgac attggcgatt cgccaggctt ccagtgcgtc tggatccttg accgcatcca
     9901 aagatgcgag cgcggacttt tcgatctcgt tcaatttttc taacatatac acctccaata
     9961 acatctacat tcaaaacaaa aacctcccgt ccacaaatgg gacgagaggc acttaagcat
    10021 ctcgcggtac cacccacgtt gaccatcctc cgcaaaggac agtccgcttg gagctgacta
    10081 acatcagcct ctcatgtaac gttgagaaaa cggttcgcac tactgtaggg caggatggaa
    10141 tcctgccgca catgttcaca caaacggctc gagagggaac ttcgactggt tttggacgag
    10201 tgtaattttc agcaggttcg gtattacaca tctctggcgg cttccgccag tctactttcc
    10261 tctgtcattg ccttttattg gggtcgcctg ttagccttta ttatctgcga aaacggaacg
    10321 gtgtcaaggc tggggttttc ctgagtattt catgcggagc gaaccacatc aattttccgc
    10381 tttcaccaaa cgataggaat aataaatccg ttcgcgattc acaaagctgt tgtacacgcc
    10441 tgatccctct actgcagcaa acgtctccat caacgggtga agaatcgcag gcgtaagtcc
    10501 atgaacaagg tcgcgatacc gctgactcgt tcgtttaagc gcgctaagga caaatgaagt
    10561 gatatccgtt ttggattcaa tattgaagcc cgcatttgta atatccctat caagttgatc
    10621 agcggtggcg ggtgtgcgaa agtcagcata caggaggcgt ccgcctttgc gaagtacacg
    10681 atagactgat ttgaaaaagg catcctggcg cgggtaacaa tgcgaggctt ccacattaat
    10741 aacggcatca aaattccctt ccggaaaatc cagcgcctgg gcatcgccca cgcgaaaatc
    10801 gatcccaagc gcgccatgag ttttgcggtc gtattcaatc gctttgggat tttgatcaat
    10861 tgccgtatag gtattgggtt gatgatagca cttcacaaag gacgctcctc ccccatgccc
    10921 acaactgact tcaagcacat ccttatcgtt gaggtcccag ccactcgcca cgtgatgata
    10981 caactggatg gcaggtcggt tgggttcatc ttgcggtttc agttcaatcg tttcgccttc
    11041 aggcggccaa tacccatagt tgagaaatgt gaccgggtca cttccaatgc gcccgctgag
    11101 atattggtac cagattttcc agaaggtttg tttgaaggat ggcattacga aatcaccccc
    11161 aacttcttcc ctaccttcgc aaatgcatcc agcccttgac ccagatcatc attatcatga
    11221 gcagcagaga tcatcacacg gatgcgcgcc ttgccctttg ccacagttgg gaatccgatt
    11281 gccatggcaa aaacgccttc atcaaataat tcgcggctga attgttgggc aagcggtgct
    11341 tcgcccaaca tcacaggcgt aatgggtgtt tcgctgacac ctgtgtcaaa gccaagcttt
    11401 ttcatctctt ccttgaagta ctttgcgttt gcccacagtt tatccactaa ctcagttgat
    11461 tcttccaata aatctactgc tgccaaacac gccgccgcgt ctggtgctgt gactgccgag
    11521 gagaataaga agggtcgtcc acgctgtcgc agccactcga tgatgacctt atcccccgcg
    11581 acgatg
//
LOCUS       DASTJP010000393         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_532646, whole genome shotgun sequence.
ACCESSION   DASTJP010000393 DASTJP010000000
VERSION     DASTJP010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532646"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFQ23_11465"
     CDS             <1..1109
                     /locus_tag="VFQ23_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9907257.1"
                     /translation="VLEFYRDAVDKGRELEAAWDANFDAYKKAHPALGADLDRRFKGE
                     LPADWDKDWPVFPVDEKGMATRVSSGKVLNVLSEKLPELIGGSADLTPSNNTRFDEAG
                     DFQKDNPMGRYLRFGVREHGMGAALNGINLFGGLIAYGGTFLVFSDYMKPAIRIAAIS
                     QIRSIFVFTHDSIGLGEDGPTHQPIEHLAALRAVPHLVVIRPADANEVADAWKYAISH
                     QNTPTVLALTRQNLPTLAETNRGLLNKGAYVLKDFGEAPQLILLASGSEVSLIYSAAA
                     KLFEEGISSRVVSFPSWELFEEQDEAYRESVLPKTLTARLAVEAGATLGWERYARSTI
                     GLDHYGASAPYKVIFEKFGFTVENVVAKAKELIA"
     gene            1164..1631
                     /gene="rpiB"
                     /locus_tag="VFQ23_11470"
     CDS             1164..1631
                     /gene="rpiB"
                     /locus_tag="VFQ23_11470"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET9907258.1"
                     /translation="MKIAVGFDHAGFPLKETVLKAIQNAGHELIDMGTNSPEPVDFPD
                     ITEKVGRAVQTGDAERGILVCGSGIGACIAANKMKGVYASICHDTYSAAQGVLHDDMN
                     VLCLGGRVIGPELAKALIPAFLNAKYLGNDSGGERLARRVEKIHKMENGESIL"
     gene            1640..3595
                     /locus_tag="VFQ23_11475"
     CDS             1640..3595
                     /locus_tag="VFQ23_11475"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HET9907259.1"
                     /translation="MPSAIEKLTSLGQSLWYDNIQRKLLETGELKAMIERGDIRGVTS
                     NPTIFNNAIAKSNDYDSALTPLAWAGWDAEKIFWQLAVEDIKAACDAFAPLYEESNGG
                     DGFVSLEVNPTLAHDTDKTVAQAEQLWVRVARPNLMVKIPATKEGIPAIRKAIAAGLN
                     INVTLIFSLKRYAEVMDAYLSGLEDRANAGHPIDHIASVASFFVSRVDSKIDPKLPED
                     SPLRGKAAIANAKLAYDEFMKTFAGMRWEKLKYKGARVQRPLWASTSTKNPNYQDTIY
                     VDNLIGAETVNTVPPATLDAFRDHGVAAMTIMRDLDEAQSSIEQLEAAGISMDLVTQE
                     LEDEGVKSFSEAFEQLLNTIDERRKGAISSLGPLAESVAKRLAQLEADSVPARLWKHD
                     PTLWTQDPAGQAEIKIRMGWLDSPEKARKLVSEYQAFAEEIKQSQIDRVLVLGMGGSS
                     LTAEVFSELLASHSSPDGRGDGGEGLRLAILDSTDPDQVAQAAAQYPPEKTLYIVASK
                     SGGTAEVMAAFDYFWKLSNEDGSHFIATTDPGTSLEALARNHNFRKIYSSDESVGGRF
                     SALTDFGLVPAALLGIDLNRLLDRVDWMKAQCGEHVPAARNPGLALGAVVGESALNGR
                     DKLTVLSDMALSPFAGWIEQIIAESSG"
BASE COUNT          888 a          910 c         1024 g          889 t
ORIGIN      
        1 atgtgctcga attctaccga gatgcagttg acaagggccg cgaattggaa gcggcctggg
       61 acgcaaattt cgatgcttac aagaaggccc atcccgcctt gggtgctgac ctggaccgtc
      121 gcttcaaagg agaattacca gcggattggg acaaggattg gccagtcttt ccggtcgatg
      181 agaaggggat ggctacccgc gtttcctcgg ggaaagtgct gaatgtatta tcggaaaagc
      241 ttccggaatt gattggcggc tcagctgatt tgactccctc taacaataca agatttgacg
      301 aagccggcga ttttcaaaag gataacccca tggggcgtta tcttcggttc ggtgtacggg
      361 aacatggcat gggggcagcc ttgaacggaa tcaacctgtt cggtgggttg atcgcgtatg
      421 gcggcacctt cctggtcttt tctgattaca tgaagccagc gattcgaatt gctgccatct
      481 cccaaataag gtcgatcttc gtgttcacgc acgatagcat tgggctcggc gaggatggcc
      541 caacacacca gccaattgaa catctcgcgg cgttacgcgc tgtgccacat ctggttgtta
      601 ttcgacctgc tgatgcgaac gaagttgcag atgcatggaa gtatgccatt tcacatcaga
      661 atacccccac cgtgctggca cttactcgtc aaaacctgcc gaccctggct gaaacgaatc
      721 gtggattatt gaacaaaggt gcgtacgttc ttaaggactt tggcgaggct cctcaactca
      781 ttttgctggc atctggatct gaagtcagcc tgatttacag tgcagcagcc aaactctttg
      841 aggaaggaat ttcttcgcgc gtggtgtcct tcccgagctg ggagttgttt gaagaacagg
      901 acgaagccta tcgcgagtcg gtgctgccga agactctcac cgcgcggttg gccgtcgaag
      961 cgggagccac acttggctgg gaacgctatg ctaggtccac tatcgggctt gaccattatg
     1021 gcgcgtctgc gccatataaa gtgatctttg aaaaattcgg gttcacagtt gagaatgtcg
     1081 ttgcgaaggc gaaagagtta attgcgtagt agcgacttca ttcgctgaca ttggacgact
     1141 gaagtcgacc ttacggagta agtatgaaaa ttgccgttgg atttgaccac gcggggtttc
     1201 cactcaagga aactgtttta aaagcgatac agaatgccgg gcacgaactc attgatatgg
     1261 ggacgaatag ccctgagcct gtagacttcc ctgacatcac agagaaagtt ggacgtgcag
     1321 ttcagactgg ggatgcagaa cgaggaattt tggtctgtgg ctcggggatt ggtgcgtgta
     1381 tcgccgccaa caaaatgaaa ggtgtgtacg catccatctg tcatgacact tattccgccg
     1441 cccagggcgt tctccatgac gatatgaacg tgctttgcct tggagggcgc gtaattgggc
     1501 cagagcttgc caaagccctg atccctgcgt ttttgaatgc gaaatatctt ggaaacgatt
     1561 ccggaggaga aagacttgcc cgtcgtgtcg aaaaaatcca caaaatggaa aacggcgagt
     1621 ctatcttata gaggtccaca tgccttctgc tattgaaaaa ctaacatctc ttggccagtc
     1681 cctttggtac gacaatattc aacgcaagtt gcttgagact ggtgaactaa aagcaatgat
     1741 cgagcgcggc gatattcgcg gggtgacttc caacccgacg atcttcaaca atgcaatcgc
     1801 caaatccaac gattacgatt cggctcttac gccactggcc tgggcaggct gggatgcaga
     1861 gaagatattc tggcagctgg cggtggaaga tatcaaagcc gcttgcgatg ccttcgcgcc
     1921 gctttacgaa gagtccaatg gtggtgatgg atttgtcagc ctggaggtca acccgacgct
     1981 tgcacatgat acggataaga cggtcgcaca agctgagcaa ctctgggtac gcgtcgctcg
     2041 cccgaatctt atggtgaaga tcccggccac taaagagggt atccctgcga ttcgcaaagc
     2101 gattgcagca ggtctcaata tcaacgtcac tttgattttt tcgctcaagc gttacgctga
     2161 agtgatggat gcttatctca gtggacttga agatcgcgcc aatgccggtc accccattga
     2221 tcatatcgcg tcggttgcgt ccttttttgt gtctcgcgtc gactcgaaaa ttgacccaaa
     2281 acttccggag gattcgcctt tgcgtggcaa agccgccatt gcaaatgcga agctcgctta
     2341 cgatgagttt atgaaaacat ttgccggtat gcgctgggaa aaactgaaat acaaaggcgc
     2401 gcgggtacag cgccctctat gggcaagcac cagtacaaaa aacccgaact atcaggatac
     2461 gatttacgtc gataacttga tcggcgcgga aacagtcaac accgttccgc ccgccaccct
     2521 ggacgccttc cgtgatcatg gcgttgcagc catgaccatc atgcgcgatt tagacgaagc
     2581 acagtcctcc atcgagcagc tcgaagccgc aggcatctcg atggatttag tgacacagga
     2641 attggaggac gaaggcgtca agtcattttc agaggccttt gaacaattgc ttaatacgat
     2701 cgatgaacga aggaaaggcg ccatttcttc acttggacct ctcgctgagt ctgttgccaa
     2761 acgccttgcg cagcttgaag cggactccgt ccctgcacgc ctttggaagc atgatcccac
     2821 cctatggact caagaccccg cggggcaggc agaaatcaag atccgtatgg gttggttgga
     2881 ttcacctgag aaagcgcgca aacttgtttc agagtatcaa gctttcgccg aagaaatcaa
     2941 gcaatcacag attgatcgcg ttttggtctt gggcatgggt ggttcatcac tcacggcaga
     3001 ggtattcagt gaattgcttg cctcgcactc ctctcctgac gggagagggg atgggggtga
     3061 gggactgcgt cttgccatct tagattccac tgaccccgac caggttgcgc aagccgcagc
     3121 gcaatatccg ccagagaaga cgttatacat tgttgccagc aaatcaggcg gcacggcaga
     3181 agtgatggca gcctttgatt atttttggaa gttaagcaat gaagacggct ctcatttcat
     3241 tgctacgact gaccccggca catcgctgga ggcactggct cgcaatcaca actttagaaa
     3301 gatatactcg tcggacgagt cggtgggtgg tcgtttctct gcgttgacgg attttggttt
     3361 ggttcctgcc gcgttgctgg gaatcgattt gaaccgtctg cttgaccgcg ttgactggat
     3421 gaaggcacag tgcggcgagc acgtccctgc tgcccgtaat ccggggctgg cgctgggagc
     3481 tgtcgtcggc gaatccgctt tgaatggccg cgataagctc acagtcctga gtgatatggc
     3541 tttatccccg tttgcaggct ggatcgagca aatcattgcc gagtcaagcg gctagaacgg
     3601 gaaggggatt ctgccggtgc cgttggaacc gttggctgat ccaaatgatt acggtgacga
     3661 tcgattgttc gtttatttac gtcaaacggg tgatctggat gaaagcatga a
//
LOCUS       DASTJP010000394         5831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1347596, whole genome shotgun sequence.
ACCESSION   DASTJP010000394 DASTJP010000000
VERSION     DASTJP010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347596"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..971
                     /locus_tag="VFQ23_11480"
     CDS             123..971
                     /locus_tag="VFQ23_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET9907260.1"
                     /translation="MPPRSGLGKGLDALIPTGKTTTSGDGNGITQVALDLIQRNPRQP
                     REKFDLEELENLAASIREHGVIQPLIVSPGKNGIYVLIAGERRLQAARKAGLRTVPVV
                     IRHATDQQLLELALIENVQRADLNSIEEAEAYQHLEKEFKLSHDVIASRVGKNRSTIT
                     NSIRLLEACAAVKQALVNGQITGGHARAMLKLSAKAQEELLNKIITLDLSVRTTEMLS
                     KKYLGQKPVTKKRTSRSANVNDLERRLQSSLGTRVALKHGKKGGTVTIYYYSDEELDT
                     LLEKLT"
     gene            complement(1053..2102)
                     /locus_tag="VFQ23_11485"
     CDS             complement(1053..2102)
                     /locus_tag="VFQ23_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9907261.1"
                     /translation="MKSIKVVLADDHSVVRAGLRNALEGLPNLEIVGEVGNGRELMDM
                     LLGVHPDLLVLDASMPEFEPISAVHQIKIKYPKLKILIVSAHHDETYVVGLLNAGVDG
                     YHFKDQPLADLQLAAQRILNGDRWISGSLIGILTSRRSSSVQPNTPWLTRRQRELLRL
                     LTQGADNRNIALTLDLSIKTVENHLTALYRVLGVDSRLKALNYALSHPELFATSGQEM
                     IETPKAKNGQELTVLVVDDNSRYRQQLCHLIGKIYPASLIFEAENSAEALLLGEKTQP
                     QLGLIDVVLEDEDGILCASRLRAVSPLTRMVVISAYPDREFKRRALSAGAIAFLDKKE
                     LDTASIRQVIEDALR"
     gene            2202..3872
                     /locus_tag="VFQ23_11490"
     CDS             2202..3872
                     /locus_tag="VFQ23_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9907262.1"
                     /translation="MAEVLLRVNGLSKTFDRLPALRDMTFTVAPGEVIGLVGRQGAGK
                     STLFNILSGTMSPSTGTIYFDGSQRRFSNRIQAQTCGIETIFQPSGPVNRFDVTNNLL
                     WDRDLGLETTRRASGLVEKFDVVSNIFLGREIVKSTPLGVIDQSRMIEISRELLAEFG
                     LSPELFREQVRNLSDEQRQILMLVRALYRPCKLFLLDDIISALSFQRQTILLEKIRHL
                     ASRGTAVIIISDNLNHLFSITDRILVLYEGRLVADRRTAASAPREIVELIVGSSRQEQ
                     VSPLIWALESYHAAQQQAEELRRMQASLQESLYAQDSLNRQLVERLRTQVLALDKLNV
                     AWQATQRRLITEREDERKALSRELHDQIIQDLIGFNYRLEEIESVEKSAVSQKAVFSI
                     RQGIRNVVEELRQMCSDLRPPTIDHHGLSAAIKSLAHEWADRNDIQLQLEIDPSLGRL
                     PEMIELSVFRIVQEGLSNVRKHAAARHVRLSLQRTASANLMVRLEDDGMGLSAPANLA
                     SLTANKHFGLVGISERVALLGGLMNIESLHGGGMILQVEIPSPSPIVA"
     gene            4319..>5831
                     /locus_tag="VFQ23_11495"
     CDS             4319..>5831
                     /locus_tag="VFQ23_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9907263.1"
                     /translation="MSKKAWNSLLAVFVIAATILSACAPAATATEAPSSELGVGIVLP
                     TKDEPRWIQDETRFRDALTSAGYDVEILFSEGDSAKERANVDSLITKGIKVLILTPQD
                     GSAAAAAAEAARAAGVKVISYDRLILDTDAVDYYVTFDSIAVGEAQAQYLVDKASGTG
                     NPLYLYAGAASDNNAFLFFEGAWNILQPKIADGTFVIKNSSEAEALQDKATLTRDEMG
                     QIIGQITTNWDFNTAKSLAESNLTAASADDKGDVFILAPNDGTARAIADAFAADSDVS
                     SYIVTGQDAEVASVQYIIDGKQSMTVLKDVRTLVSDAIAAAVTFLDGSTPEQTTTYNN
                     GAIDVPAKPSEVITVDQSNVQEAIIDSGYWPASEFTGLDTLGAAATEPPAAAASGEVE
                     IFSWWVGGGEAAGLTAMTEVFAQEYPDIEFVNAAVAGGSGTNARAVLATRLQAGDPPD
                     SWQAHAGQEIIGTYVAADQVAPLDDFFAETGFADVLPETLLPLISQDGSPYSVP"
BASE COUNT         1448 a         1430 c         1487 g         1466 t
ORIGIN      
        1 tgttggcgcg caagcatatc aatcgctcgc aaaagaattg ctccatggtg atggagttgc
       61 tgaaagataa ttggtataaa tggtcaccag cccatctcta tactgaatct taaggagaaa
      121 tcatgcctcc acgttccggt cttggcaagg gactagacgc tcttatcccc actggtaaga
      181 cgacaacatc aggggatgga aatggtataa cacaggtcgc tctggacttg atccagcgca
      241 acccgcgcca gccgcgcgaa aagttcgatt tggaggaatt ggaaaatctt gcggcctcca
      301 ttcgtgaaca tggggttatc caacctttga tcgtttcgcc gggcaaaaac ggaatctacg
      361 ttcttatcgc aggtgaacgt cgcctgcaag ctgcgcgcaa agcagggtta cgaaccgtcc
      421 ctgtcgtgat acgccacgcc actgaccagc aattacttga actggcattg attgaaaacg
      481 ttcagcgtgc tgacctgaac tcgattgaag aagcggaagc gtaccagcat ctggaaaagg
      541 aattcaaact ttcacatgat gtgatcgcgt cccgtgtcgg gaaaaaccgt tctacgatca
      601 ccaactccat tcgactactt gaggcctgcg cagcagtgaa acaagcattg gtaaatggtc
      661 aaatcactgg gggtcatgcg cgtgctatgc tcaagttatc tgccaaggca caggaagaat
      721 tactcaataa gatcattact cttgacctta gtgtgcgcac aactgaaatg ctctccaaaa
      781 aatatttggg gcagaagcca gtgaccaaaa aaagaaccag cagaagcgcg aatgtcaacg
      841 atctcgaacg ccgcctgcaa tccagcctgg ggaccagagt tgcgctcaag catggcaaga
      901 aaggtgggac agtcactatt tattactatt ccgatgaaga actggatacc ttgctggaaa
      961 agttaacgtg atttcttggt gcaaatgatg gaaaggtaac aagaaatcac ttgttaaatc
     1021 cccttcgatc attgagaaga aaccctctta tatcatctca gcgcgtcttc gataacctgt
     1081 cgaatcgacg cggtgtccag ttcttttttg tctaaaaatg caattgcacc ggcagacaat
     1141 gcacgtcgct taaattccct atccgggtac gcgctaataa ccaccatgcg cgtgagtgga
     1201 gaaacggctc gcaagcggct tgcgcagaga attccatctt cgtcttccag aaccacatcg
     1261 atcagtccaa gttgaggttg ggttttttcg cccaacagga gcgcctcagc cgaattctct
     1321 gcttcgaaga ttaaggatgc ggggtaaatc ttgccaatga gatgacaaag ttgctggcgg
     1381 tagcgcgagt tatcgtctac taccaatacc gttaattcct gcccgttttt ggctttgggc
     1441 gtctcgatca tttcctgtcc gcttgttgca aacaattcag ggtggcttag ggcataattt
     1501 aaagccttga gcctactatc cacgcccagc acgcgatata aggctgtcag gtggttttca
     1561 acagttttga tacttaagtc taacgtcaat gcaatgttgc gattatccgc gccctgcgta
     1621 agcagccgaa gcaattcgcg ttgtcgccgg gtaagccagg gagtgttggg ttgcaccgac
     1681 gaagaccttc gactggtcag tatcccaatc agacttcccg agatccaccg atctccattg
     1741 aggattcgct gggcggctaa ttggagatca gcaaggggtt ggtccttaaa gtgatagcca
     1801 tccacaccag cattgagcaa tcccactaca tacgtctcat cgtggtgggc actgacgatc
     1861 aggattttca atttaggata cttaatcttg atctggtgga ccgcggaaat cggttcgaac
     1921 tcaggcatgc tggcatcgag aacgagcaaa tccgggtgga caccaagcaa catgtccata
     1981 agctcacgac cattcccaac ctcgcccaca atttcaagat tcggtaagcc ctccagggca
     2041 tttcttaacc cagcccggac caccgaatga tcatcagcca gcactacttt gatggacttc
     2101 atgaagctat tatgccgcaa gtgatatcaa ctgactagga gagttagact cctggttctt
     2161 tgcatttttt cttttctctg aattcaggtt aaaatgattg catggctgaa gtactcttgc
     2221 gtgtaaacgg tttatcaaaa acgtttgatc gtttgcctgc attgagagat atgaccttta
     2281 cggtcgcgcc aggggaagtg atcggtctgg tgggccgtca gggagccggg aaatctacat
     2341 tatttaacat attgagcgga acaatgtctc cgtctacggg gacgatatat tttgatggtt
     2401 cgcagcgtcg cttctccaac cgcattcagg cgcaaacatg tggtatcgaa accatatttc
     2461 agccatctgg tccggtcaat cgcttcgatg tgaccaacaa tctcctttgg gaccgagatc
     2521 ttgggcttga gacaacgcgc cgcgcctccg gtttggtgga aaagttcgat gttgtctcca
     2581 atatctttct tggtcgtgag atcgtgaaat caactccgct gggggtgatt gaccagagcc
     2641 gcatgattga aatttctcga gaattgctgg ctgagtttgg gctgtcgccg gaattattcc
     2701 gggaacaggt gcggaatctc tctgatgaac aaaggcaaat cctgatgctg gtccgcgcgc
     2761 tgtatcggcc ctgcaagctt ttcctgctgg atgatattat ctcggctttg agttttcagc
     2821 gtcagaccat cttattggaa aaaatcaggc atcttgcctc ccgggggaca gctgtcatca
     2881 tcattagcga taacctcaac catttgtttt ctataacaga ccggattttg gtgctctacg
     2941 aaggacgatt ggtcgcggat cgacgcacag ctgcctctgc gcctcgtgaa atcgttgagt
     3001 tgatcgtcgg ttcgtcgagg caggaacaag tctcgccact aatctgggcc ctggaaagct
     3061 atcatgctgc acaacaacag gcggaggagc tgcgtcggat gcaggcctcg ttgcaggaaa
     3121 gtctgtatgc acaggattct ctgaacaggc aactagtgga gcgtttgcgt acgcaagtgc
     3181 tcgctcttga caagctaaat gttgcctggc aggctacaca gcgcaggtta attactgaac
     3241 gtgaagatga gcgaaaagct ttgtcccgtg aattacatga tcagattatc caggatttga
     3301 tcggttttaa ttaccgtctt gaagaaatag agtctgttga gaaatctgcg gtttcgcaaa
     3361 aagcagtttt ctccattcgt caggggattc gaaatgttgt agaagaatta agacaaatgt
     3421 gcagcgattt acgtcctccg acaattgatc atcacgggct ttcagccgca atcaagtcac
     3481 ttgcgcatga atgggccgac cgcaacgata ttcaactcca actggaaata gatccttctt
     3541 tagggcgttt gcctgaaatg attgaactat ctgtgttccg gattgtgcag gaggggctga
     3601 gcaatgtgcg taagcatgcg gctgcccgtc acgtgcggct ctcattgcag cgtacggcat
     3661 cagccaacct tatggtccgc ctggaagacg atgggatggg cttatcggcc ccggccaacc
     3721 ttgcatctct cactgcaaac aaacactttg gcctggtggg aatcagtgaa cgggtggctt
     3781 tactgggcgg gctgatgaat attgagtcgt tgcacggagg gggaatgatt ctgcaagtgg
     3841 agattccaag tccatcccca attgtggcat aatagccctc cgaaaacagt ctgagctttt
     3901 tctgggtctc aatccaattc cggcacaatt agggggtttc ccctaaccct taaaggggta
     3961 tactcttatt gaacccaggg atttggatat atgcaggcaa cggataagaa ataaaataat
     4021 agggtcgagc atagttaagt gagtttggtc tttttttgac taagtggcgt tctgtcaaat
     4081 tattcgactc atggatttcc ctggttcctg ttccactctc atgaattatg cataacttct
     4141 caacccacct ggaactacat ttgatttttc cacgccgaaa catatcccgt tcatgttaac
     4201 gggttgtttc actttcatag ctgattggtc attttatata aaggaggtga tggctgacaa
     4261 gaaatccgta tggtagtaat ttgactctat attcaacaat ctagataagg agaagaaaat
     4321 gtctaagaaa gcttggaata gcctcctggc tgtttttgtc atcgccgcga caatcctctc
     4381 tgcctgtgct cctgcagcta cggcaacaga ggcgcccagc tccgaactgg gagtcggtat
     4441 tgttcttccg acgaaggatg aaccgcgctg gatccaggat gaaacccggt tcagagatgc
     4501 gctcacctcc gccgggtatg atgtggaaat cctcttcagc gaaggcgatt ccgcgaaaga
     4561 acgcgcgaat gtcgattcac tgatcaccaa aggcattaaa gtcctcatcc tcaccccgca
     4621 ggatggttct gctgccgcag cagcggctga agccgcccgc gccgccggtg tcaaggtgat
     4681 ctcgtatgac cgtcttattc ttgatacaga tgctgtggac tattatgtaa cgttcgacag
     4741 cattgcggtg ggtgaggctc aagcgcaata cctggtggac aaggcgtccg gcacagggaa
     4801 cccgctgtat ctttatgcag gcgcggcctc tgacaacaat gccttcctat tcttcgaggg
     4861 tgcctggaat atacttcagc ccaagattgc agatgggacg ttcgtgatca agaattccag
     4921 cgaagcagaa gctctgcagg acaaagctac gttgacacgt gacgaaatgg gccagatcat
     4981 tggtcaaatc accaccaatt gggatttcaa caccgcgaag agtttggcag agtccaactt
     5041 aactgcagca tctgctgacg ataaaggcga tgtcttcatc ctcgctccaa acgatggtac
     5101 tgcccgcgcg attgctgatg cttttgcagc agatagtgat gtgagcagct acatcgtgac
     5161 cggtcaggat gccgaagttg cctccgtcca atacatcatt gacggaaaac agtccatgac
     5221 cgttttgaag gatgtccgca ctctggtgtc agatgccatt gctgctgccg tgacattcct
     5281 cgatggaagc acacctgagc agaccacgac ctacaataat ggcgcgattg acgttcctgc
     5341 gaaaccctcc gaggtgatta cggtggatca gagcaacgtt caggaagcca tcattgactc
     5401 ggggtattgg cccgccagtg agttcaccgg cttggataca ttaggcgctg ctgccacaga
     5461 accccctgct gctgcagcca gcggcgaggt ggagattttc tcgtggtggg ttggtggtgg
     5521 tgaagcagca ggcttgaccg ccatgaccga ggtcttcgcc caggaatatc cagatattga
     5581 gttcgtgaac gctgcggtag ctggcggctc tggtaccaat gctcgcgccg tccttgccac
     5641 ccgtttacag gctggcgacc cgcccgattc atggcaggcg cacgctggtc aggaaattat
     5701 cggtacgtac gttgcagcag atcaggtagc cccactggat gatttcttcg ccgagacggg
     5761 ttttgctgat gtcctgccag aaacactcct gcctttgatt tcacaggatg gaagtcctta
     5821 ctcagtgcca g
//
LOCUS       DASTJP010000395        18275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_992737, whole genome shotgun sequence.
ACCESSION   DASTJP010000395 DASTJP010000000
VERSION     DASTJP010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992737"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1178)
                     /locus_tag="VFQ23_11500"
     CDS             complement(228..1178)
                     /locus_tag="VFQ23_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907264.1"
                     /translation="MNINRSALFWGILLTAAGLLALGDQLGYTDTFSPQTWIIIFGVT
                     SLVGLVSYALSGWKEWAWLFPAGIFGGLALIVGLAESGSENAAVASPLFFGLLIPFVA
                     AYLTDRTRNWWAVIPGGVMLFLALTTLLVDSVRGEWVGALFLFLIALSFLAVYLNDRK
                     RIWAVLVAYIFGVLSIAPMMASGGETVAYYGPIFLFAVALPFFILYLRSGNYWWAIIP
                     AGAVTTVSVIAALAIAGLIQSETEGGFVSAIMMSGLAVPFAVLWLRHAKQWAKIATIG
                     LLVLALGSIFFATYTQILWPLAIVVVGMYLFFTALRPRHA"
     gene            complement(1239..2168)
                     /locus_tag="VFQ23_11505"
     CDS             complement(1239..2168)
                     /locus_tag="VFQ23_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907265.1"
                     /translation="MRTAASLHLSPIQTVGKAFSVFLRFVILVILYFALWSMGGRIIA
                     TYIPQNPAEPGPVPLLTGLLIACATSVLVIMLMISSSRWYGWKLMISMSVAFYLVMTL
                     VTQLESWYFLYGITIGSQLMLGLFLQGLPLAFVFVPIAVLLMGRFRRTEHQADDSKIV
                     SMPISEWFWKLGLIYVAYLVLYFTAGYFIAWQNPELRAFYGSPGEALPFFQHMRNVFL
                     TDPWLTPYQFLRTLIWVAGAYLIIRGSRLPLWQTALIVGLVLSVPQNIAHILPNPLIP
                     LNSVRMSHLLETASSTFIFGLIVTWLLYPKRTC"
     gene            complement(2434..3429)
                     /locus_tag="VFQ23_11510"
     CDS             complement(2434..3429)
                     /locus_tag="VFQ23_11510"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HET9907266.1"
                     /translation="MLGNFINQTPLSKPSPSFYVREIPIYGDTVLAPMDGYSDWPFRS
                     ICRALGSAMSYTEFVKVEKILSRSKEPAKRLYYEEAERPITFQIYGDDPDLILKAALR
                     VEKWKPDIIDINMGCPAKSIADRGAGVGMMPTPLKIARTFKKLTSALKVPVTGKIRLG
                     WDKDKNYKLIARVIEENGGSLIAIHGRTKEQRYSGNANWDAIAEVKSLVKIPVTGSGD
                     VRTVADIERMKQHTNCDAVMIGRGAIANPWIFMGFDREQVPNELLKETVHEHLQKSIQ
                     FYGEEDGQRLFRKYAVQYLLLRTLDRDARKEILKERPTGEFLEILNQVYTLTARL"
     gene            3501..4442
                     /locus_tag="VFQ23_11515"
     CDS             3501..4442
                     /locus_tag="VFQ23_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9907267.1"
                     /translation="MKYIVILTLGLFVVASGCAPKTDTPLIEVAPTVSGESSGFFFDD
                     FTYSALDEMTANGWIVRSGSGWPGVTGATFRLENVSFVDYPDLAGNRLLRMTSSTDGT
                     SANTFQTQVCHQRKYLEGTYAARVRFSDTPTFGRDGDQIVQTFYTITPYDRPFNPAYS
                     EMDYEYLPNGGWGGPELIFHVTTWETVQIEPWDADNKSNSILGSLAGWHTLVTQVMGG
                     TVRYFIDGDLIAQHGGAYYPDAQMSINFNLWFIEGGLVQSEGVREYQEDVDWVFYQAG
                     ILLMPEDVEAKIDELRDASVNFEDTVESGIQVSPCDL"
     gene            complement(4505..5056)
                     /gene="msrA"
                     /locus_tag="VFQ23_11520"
     CDS             complement(4505..5056)
                     /gene="msrA"
                     /locus_tag="VFQ23_11520"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET9907268.1"
                     /translation="MNTNLQTATLAGGCFWCLEAVFDEVKGVQGVESGYAGGHVDNPS
                     YRAVCNGDTGHAEVVQVHFDPSIVSYRDLLNVFFAIHDPTTLNRQGADVGTQYRSAIF
                     YHDDEQKKVAEELIKDLNTQKIWDRPIVTQVEKLDKFFMAEDYHQEYFVRNQYQPYCM
                     AVVAPKVSKFRKHFLELLKKQPA"
     gene            5185..5994
                     /locus_tag="VFQ23_11525"
     CDS             5185..5994
                     /locus_tag="VFQ23_11525"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9907269.1"
                     /translation="MEKSSYDKVWHWFLTGEKNGKFPLSVELWLKFSHTFYKWFPGDP
                     RCFECEMPLAGLSSVLLRPMGSRPSSFSSRLCSACENFVRQHEGGAEVEMTLLFADVR
                     DSTPLAEAKGTLGFKDIINRFYKEASDVLVAHHAMVNRLMGDQIIALFVPRFAGEAHA
                     KVALHATQELLRVTGHRNADGPWVPVGAGIHTGLAYVGAVGSAKGVNEIAVLGSAANM
                     CARLSSKAATGEILISEDSVKSGNLDVKDLESRSLELKGVSQPVSVRAIKV"
     gene            6084..6953
                     /locus_tag="VFQ23_11530"
     CDS             6084..6953
                     /locus_tag="VFQ23_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9907270.1"
                     /translation="MTILSTPHPIEADTETQMKVRRARQGLLVFACLLIPLSLFGYWF
                     YVNFPDAPLVLPSLPLMLSPGLAAILTRFLRREGFADVSFRLRGPRIERAYLLAFALP
                     LVVGGAAYGLAYLLGLARFDPPPFPFAVSSPIAQFALNVIFSLILIILLLPSAGGEEI
                     GWRGYLLPRMIDSGIQKPILLTSLIWGAWHLPVVFAGVYAVGPSPLLSAAGLMIATLA
                     FGSILAWIRLGTGSIWPCIIAHAAWNAIINGAFTPATQNATENAWVGETGILVAVALV
                     VAAVWLGRSWGSR"
     gene            complement(7059..8375)
                     /locus_tag="VFQ23_11535"
     CDS             complement(7059..8375)
                     /locus_tag="VFQ23_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HET9907271.1"
                     /translation="MTKIAFIGAGSTVFAKNLIGDILSFPELADVSISLMDINPERLH
                     TSEIVANKVANFLGAKPQIEATSDRRRALDGADYAISMFQVGGYDPGTIVDFEVPKRY
                     GLRQTIADTLGIGGIMRGLRTIPVFLDICRDMEELCPDVMFLQYVNPMAMNTWAISRA
                     SKIKTVGLCHSVQGTAEALAKDMGLPYDEINYICAGINHMAFYLRFEHNGQDLYPRLQ
                     QVLEAKSYGRRSDGLADHVRYEVFRRTGYFVTESSEHFSEYVPWFIKRDRPDMLEQFE
                     IPLDEYIRRCELQIAGWKAVKDSLERGEISEIQHERSHEYGSLIIHSMETGEPRVVYG
                     NVPNHGLIDNLPQGCCVEVPCLVDKSGLQPTKIGLMPPHLAALQQTNINVQSLVVEAA
                     LTGKREHIYHAAMLDPHTAAELSLDQIWSMVDELIETHGDMLPAYH"
     gene            complement(8482..10965)
                     /locus_tag="VFQ23_11540"
     CDS             complement(8482..10965)
                     /locus_tag="VFQ23_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET9907272.1"
                     /translation="MAGMERFTQRARRVLSLAHQEAERARNNNIGTEHLLLGLMDEEG
                     GVAGRVLRELGMTSDRVREVIRRVTNSSTSFDPSRVELASDTQQVLEHAVDEARRLGH
                     HYIGTEHILLGLVRVDSAAMEVLRRLGVTAEQIRRQTRRVLNESTSSSPTPAGSGSSR
                     SSAANPKTPLIDQLASDLTTKAEEKKLDPVIGRQMEIERVIQILARRTKNNPALIGEP
                     GVGKTAIVEGLAQRIVDGDIPAPLMNKRLLQLDVGSLVAGTMYRGQFEERLKRVIDEL
                     KQSGAILFIDEVHMLVGAGAAGSSVDAANILKPALSRGELQVIGATTMDEYRKYIESD
                     AALERRFQPIQVDEPSEEETIEILKGIRSAYEEHHHLVISDEALEAAAHLSSRYVTER
                     FLPDKAIDLIDESSSRVRMYKSDAAKEAKELFGQLRSARQNRSLAQEEGNSEDVKEWE
                     DRELELGEQIERLRTGWDRASSPVVSAEDIAEVVSMWTGVPLMQLAEEESERLLHMEA
                     ELQKNIIGQDEAIGAISRAVRRARAGLKDPRRPIGSFIFLGPTGVGKTELTKTLAKFM
                     FGSEDAAVQLDMSEFMERHTASRLVGAPPGYIGYEEAGQLTEALRRRPYSIVVFDEVE
                     KAHPEVHNMLLQIMEEGHLSDAKGRKVDFRNAIIVMTSNIGADVIKRQSSLGFALKRD
                     EVTEERLSYEDMRKKLSESLKRAFRPEFINRLDSVVIFRSLNKEDIQHIVALELDKVA
                     ERIKEHNLVLTATPEALSALAEQGYDAEFGARPLKRVIQQKVENPLSDRLLNGEFQHG
                     DTVRVILNAEGEIALERDREPEAAEAPAV"
     gene            11077..11667
                     /locus_tag="VFQ23_11545"
     CDS             11077..11667
                     /locus_tag="VFQ23_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET9907273.1"
                     /translation="MTVIKAICIFCGSSDSVHADYLTAARSMGLTLAERGIRLIYGGG
                     KTGLMGAVAKGTLEAGGEVIGVIIPSMNTISLAYDGLTRMDVPPDMHARKARMHELAD
                     AYIALPGGFGTFDELFETVTWAQTGAHEKPVGLLNVRNYYAPLLAAIDHAVEEGFVFK
                     EHRDSLFIDSDPNTLIDKLTEYIHPHDAVKRWMRED"
     gene            complement(11678..13399)
                     /locus_tag="VFQ23_11550"
     CDS             complement(11678..13399)
                     /locus_tag="VFQ23_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907274.1"
                     /translation="MLTTLDNRGNAVFTPPSWVVILLIGCILVVETFVFLLPAPIEFP
                     MDDTYIHFVYADNLVSEGKLFFSDVNEKGVGATSPLWVFLLAGFKLLGIPLFVSAKAL
                     GMIGLAVVSGGIYSLFRPVWKSPFLLLAVVLLSISGNLIWFSLSGMETMLFLALAILS
                     LLAYREQKWKLFGILLGLLILVRPEGVILAASVALVDIWAQRSLRRELVFAFLIGFII
                     SVPWFIYLYLRTEHFMPTSAIGKRFTFDLGLDYVAAQNPYLSSFVQLRSLVYPLAWLA
                     YRLVFALGGKSLPAPYIAENSGFGISSYSPSYWSILGWLVVIFPLLLAASRWFVVRRE
                     WAGWVERSNARPLMIFALWFLLHNLAYMLFMPILGTASRYGALNHVVVWIILAFGVSR
                     LAQPGYLARFMTVGLLIIAVANNLYWNRVYDANIEHMVQVRIATAHFVRDSLPEKDHC
                     AVFDIGAIRYFSQQPIVDIGGLTDPDEYEWFIANKTDLFLMEHGVTCLILPGQADIAG
                     EGWLDFLHIMGIDDSPYFKLQQIATFEMDAERWLVGYLPTSNQQKSVVVYRLIEAKSG
                     LPTEVTP"
     gene            complement(13520..14779)
                     /locus_tag="VFQ23_11555"
     CDS             complement(13520..14779)
                     /locus_tag="VFQ23_11555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9907275.1"
                     /translation="MRIAYLAQSYPPMVSGAAIAAEQLANNMAMRGHQVLVIAASDKD
                     QPYITVQENIQVLRLKSMYNPMRVGQRFLLFPRYPVKRALEEFQPDLIHCHEPLQIGL
                     LGLAYARRAQVPSSLTVHQLPWFVAAYLPSFLRTAVEKSLWSYARWLLKRFTSIIAPT
                     KSISTLIHQMTGLEAKTIGYGLDLNVFHPPFSMDMTIEIRQKWDLPAHVPLMLHVGRL
                     DTEKHVDHVILAAALAMQHTDAHLLIVGDGRQKQHLMKICRDLNMEGRVHFTGYVSVQ
                     QGLPELYRIACMFVTASEIETQGIVLLEAAASGLPLVAVDATCIPEIVYDGANGYLTK
                     AGDAYAMGQSMIRILSDPWLAGSMGRVSRTLAAGHPKQATFDEHERMYQQMITEYGTK
                     STSARQSAHLQWKQFKKRLIWAFTGWF"
     gene            complement(14737..16086)
                     /locus_tag="VFQ23_11560"
     CDS             complement(14737..16086)
                     /locus_tag="VFQ23_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET9907276.1"
                     /translation="MKNNYLFKFILFLFGLLILWISRDSISSLWAWIGDRETVTSSME
                     KAGLWGPVVLFVLFVLQVFLAFIPGQALMVACGYLYGFWGGFLLSWLSLVIGGEIAFL
                     LARHHGRGFAEKWISKDVLTRWDRSAEGQGIVFFTLSLVIPFLPNDAMCYVAGLGKIS
                     HRRFSLANLLGRGLACIVMSWIGAFGMQAPPHFWMIVIVLCAGVVFLMLCKKAGISPQ
                     IKKKLANAAGMLIVRTYQRIFRLRYEVKGLESLPAGPKIIAINHTNVTDIVFLPLALS
                     SIPRFVVQGDLFEVPVLGEILKGTGQIPVNAGDRPKAFEQAHEFLKQGETILIFPEGQ
                     LVPFGERVKAWTGTVRLALATGAPIIPLGIYADPQNVAALKIHRNGQARKGHYQFRGN
                     CYLRFGAAWQPDPSKKKPVQVHAQTDELMDKIYSLVAEAQKESQCVSPTLLNPIPQW"
     gene            complement(16222..18060)
                     /locus_tag="VFQ23_11565"
     CDS             complement(16222..18060)
                     /locus_tag="VFQ23_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907277.1"
                     /translation="MTLYQVTFTVNFVALIASLWLGMYLVTRNPRVPTAWLTALTLWS
                     LGGLFFNVLLALNPPPDPTFRPAGLRFLFPFWPSGEVEDKATAWLQGWSVIPSIAFWH
                     HATLLMRPGKMNPWRWTRVVFTYIVAIAAIAVQTFTQILFSVEGGDPLYLNSLRAGPL
                     YSVFGVALIALTIMSIINLARSAYVAPTEIVRRQLLTLVGATVFTGLIGPVSLVSSGL
                     DLFPMPMVVMSGVLAVFVAMIGYGVARYSALVEKRTIDRDFFYNLIGVIVMVSIYTLA
                     AWVLVFAYQAPRVIVIVFPLLGVLTHTGLNAASIFLDWFFYRGETRRLRFNLHSLRRL
                     AGESENMKILLEQILGALCVPIKATYALVLTFEDATARQVADFRWKNPRLELPSGMLT
                     ADDVLHLTPKQFPAPLEDAALLIPLYIESEQIGALVLGRPTNGLRYAHADLERLLYPS
                     DQIAEALFLSRQNTERLEKVALLADAQPAPPAREFNIPVNVVENALRNLFDYAYLADT
                     PMAEMELVRARLAAGKKTHLERGKTVQAVLLEALEHMRPSSEVPRDPPPREWYPYVII
                     HDAYVNGKQNRDVMSRLYISEGTFNRTRRAAITSLARALVEMEHPS"
BASE COUNT         4667 a         4608 c         4547 g         4453 t
ORIGIN      
        1 ttatccgata cattgcgggc tgcttctgct tgccacctgc tgtagtattc acctgctggt
       61 atttgtttcg cttctctatc tgatagcagg cgagcaatgt tgcctttcat atatttatcc
      121 atggaagacc tccttccatt cttaggatag ccatcttgaa caaaagactc tcccatcact
      181 gggagggtct tctctgtccg attagacagt gtggtcgcat agacagatta agcatgtctc
      241 ggacgcagcg cagtgaagaa cagatacata cccaccacta caattgccag cggccacagg
      301 atctgtgtat aagtagcgaa gaagattgaa ccgagtgcca gcaccagcag cccgatggtg
      361 gcaatcttag cccattgctt cgcatgtcgc agccagagga ctgcaaaggg tacagccagc
      421 ccgctcatca tgatggcact gacgaatcca ccttctgttt cactttggat cagtccagcg
      481 attgccagcg ctgcaatgac tgacaccgtt gtcactgcac cggccgggat gatcgcccac
      541 cagtaattcc cacttcgcag ataaaggatg aagaacggaa gggccaccgc aaagaggaaa
      601 atggggccat aatacgccac cgtttcgcca ccggaagcca tcatcggcgc aatactcaat
      661 actccaaaaa tataggcgac gagtactgcc catatgcgct tgcggtcatt caggtacaca
      721 gccaggaagc tgagcgcgat caggaagagg aacaacgcgc caacccattc accgcgcacc
      781 gaatcaacca gcagcgtagt caatgccagg aagagcataa cgccgccagg gatcactgcc
      841 caccagtttc gtgtgcgatc ggtgagatat gctgcaacga atgggatgag caaaccgaag
      901 aacagcggcg aggcaacggc tgcgttttcg gatcctgatt cagccagacc aacgatgagc
      961 gccagaccac cgaagatgcc agctgggaac aaccatgccc attctttcca cccactgagg
     1021 gcatagctaa ccagccccac cagacttgtg acaccaaaaa tgatgatcca tgtctgtgga
     1081 gagaaggtgt cggtatatcc caattgatcg cccagcgcca gcaagcccgc ggcggtcagc
     1141 aggatacccc agaataaagc cgatcgattg atgttcatga gattctcctt gcgatttgaa
     1201 tatgcttcga tagaaatgcg attcgcgtgc agtggaggtc agcatgtgcg tttagggtac
     1261 aaaagccagg tcacgatcaa gccaaagatg aaggtcgagg atgccgtctc gagtaaatga
     1321 ctcatgcgta cactatttag tgggatcaaa ggattcggca ggatgtgagc gatgttttgc
     1381 ggcacactca ggactagccc aacaatgagc gcggtctgcc agagcggcag gcgtgaccca
     1441 cgtatgatca ggtaagcacc agcaacccaa atcaaagtgc gcagaaactg gtacggagtc
     1501 agccacggat cagtcaggaa cacatttctc atatgctgga agaaaggcag tgcttcgccc
     1561 ggcgagccat aaaacgcacg tagttcagga ttctgccagg cgatgaaata gcccgctgtg
     1621 aagtagagca cgaggtaggc aacatagatc aaaccaagtt tccagaacca ctccgatatg
     1681 ggcatggaaa caatcttcga atcgtcagct tgatgttctg tacggcggaa acgtcccatt
     1741 agcaggacag caattggtac aaaaacaaat gccagcggta atccttgtag gaacaacccc
     1801 agcatgagct gggagccgat agtgattcca tataggaagt accaggattc gagttgggtt
     1861 acgagagtca tcacaagata gaacgcaacc gacattgaaa tcatcaactt ccatccatac
     1921 catcgtgaac ttgatatcat tagcatgatc accagaacgc tggtggcaca agcgatcaac
     1981 agaccagtca gcagcggtac gggtcccggc tcggccggat tttgaggaat atatgttgcg
     2041 ataatccgcc cacccatgga ccagagtgca aagtaaagga tgaccaggat tacgaatcgt
     2101 aaaaaaacgc tgaaagcctt tccaacggtt tggatgggcg agagatgcaa actggctgct
     2161 gttctcatgg tatgctcctt aatagatgga ttggatttcg acaacataag tttaatgaga
     2221 tagaaataaa gactcccctc ctcacagggg agtcttttac tagtcaagtg gacagccttg
     2281 tctggattct ttttcaaggc atgcggcact catcagccgc gcattcgcac cttgccacct
     2341 caggcgcaat gctcgggttt gtagttatga tgattttgga tgttgcgttg ggattaagac
     2401 tgcgtttctt cgcgtacctg gtgaactttg cttttacaac ctcgcggtta acgtatatac
     2461 ctggttcaga atctccaaaa attctccggt aggtcgttcc ttcagaattt ccttgcgtgc
     2521 atcgcggtcc agcgttctca acaggagata ctgcacggcg tatttgcgaa agagcctttg
     2581 accatcctct tcaccataga attggatgct tttttgtaaa tgttcatgaa ccgtttcttt
     2641 cagtagttca ttgggaacct gttctcgatc gaagcccata aagatccagg gattggcaat
     2701 cgcgccgcgc ccgatcatga ctgcgtcaca gttcgtgtgt tgcttcattc gttcgatatc
     2761 ggctacagtt cgtacatcgc ctgagcctgt caccggtatc ttgaccagtg actttacctc
     2821 ggcaatggca tcccagtttg cgttgccact ataacgctgt tctttcgtcc gcccatgaat
     2881 ggcgatcagc gagccgccat tttcttcgat gacccgcgcg atcaacttat agtttttatc
     2941 cttatcccat cccaaccgaa tctttccggt gacagggact ttgagcgcag aggttaattt
     3001 cttaaacgtg cgagcgatct tcaagggcgt gggcatcatc cccacacccg cgccacgatc
     3061 tgcgatgctt ttcgccgggc aacccatgtt aatgtcaatg atgtctggct tccacttttc
     3121 cacccgcagt gcggctttca ggatgagatc agggtcgtca ccataaattt gaaaggtaat
     3181 tggacgttct gcttcttcgt agtagagtcg ttttgcaggt tccttcgacc gactcaaaat
     3241 cttttccacc ttcacgaact cggtataact cattgcagag cccagggcgc ggcaaataga
     3301 acggaagggc caatccgaat agccgtccat gggtgcaagt actgtatctc cgtaaatagg
     3361 gatctcgcgc acataaaaag acggagatgg ttttgacagc ggtgtttggt tgataaaatt
     3421 acctaacata gccttatgag tataacagaa tgtctgcccg tcggaattca gagctggtat
     3481 caggaatacg gatgataaaa atgaaatata tcgttatcct cacccttgga ctttttgttg
     3541 tagcaagtgg ctgcgctcct aaaactgaca cccctctcat cgaagtagcg ccgacagtat
     3601 caggcgaatc ctcaggattc ttcttcgacg atttcaccta ctccgcattg gatgagatga
     3661 ctgcgaacgg ctggatcgtc cgtagtggct ccggttggcc cggagtaacc ggcgcaacct
     3721 ttcgacttga gaatgtttcg ttcgtcgact atccggatct ggctggcaat cgtctcctgc
     3781 gaatgacatc ctccacagat ggcacaagcg caaatacgtt tcagacgcaa gtttgtcatc
     3841 agcgtaaata tctggagggt acgtacgctg cccgagtccg ctttagtgat actcccactt
     3901 tcggaaggga tggcgatcag attgttcaaa cgttttatac catcactccg tatgatcggc
     3961 cgttcaaccc ggcctatagt gagatggatt atgaatacct gccaaatggc gggtggggag
     4021 gcccggaact gatatttcat gtgactacct gggaaacggt gcagatcgag ccgtgggatg
     4081 cggataacaa aagcaattcc attctgggaa gcctggcggg ctggcataca ctggtcaccc
     4141 aggtaatggg cggaacggtg cgttatttta ttgacggcga tttaatcgcg cagcacggtg
     4201 gagcttacta tccggatgct cagatgtcca ttaatttcaa tttatggttt attgaaggtg
     4261 gattggttca atcagaaggt gttcgcgaat atcaggagga tgttgactgg gtgttctatc
     4321 aggcaggcat tttgctcatg ccggaagacg tcgaggctaa gatcgacgag ttaagagacg
     4381 cctccgtaaa ctttgaggac actgtggagt ctggaattca ggtgtcgcct tgtgatctat
     4441 gatataaaat ttttgtccct taggattttc gaaaatccta agggactttt tgacttttag
     4501 acttttacgc aggctgcttc ttcaacagct ccaggaagtg cttgcggaac tttgatacct
     4561 tcggcgctac cactgccata caatagggct gatattgatt tctaacgaaa tactcctgat
     4621 ggtaatcctc cgccatgaag aacttatcca gcttctccac ctgtgtaaca atcggacgat
     4681 cccagatctt ctgggtattc aaatccttga tcagctcctc agccaccttc ttctgctcat
     4741 cgtcgtgata aaagatagcg gagcggtact gggttccaac atcagcaccc tgtcgattca
     4801 atgtggtcgg atcgtgaatg gcgaagaaga cgttgagcag atcgcgatac gagacaatgc
     4861 tcgggtcaaa atggacctga acgacttcgg cgtgtcctgt gtcgccgttg caaacagcgc
     4921 gataggatgg gttatccaca tgcccaccgg catagcctga ttccaccccc tgaactccct
     4981 ttacctcatc gaaaacagct tcgagacacc agaaacaccc ccctgccagc gtcgcggttt
     5041 gaagatttgt gttcatatga ttactccttt tcttgatctg cggcttagac aacataagag
     5101 agtaaaatct tattaagaaa gtattacggc tattttcagg gttatatagc tctcataccc
     5161 cttcctacga aatgagaaat gtgaatggaa aaaagttcct acgataaggt ctggcattgg
     5221 tttcttacag gcgagaaaaa cggaaagttt cccctctctg tggagttgtg gttgaaattc
     5281 agccatacct tttacaaatg gtttcccggg gatccgcgat gtttcgaatg tgaaatgcca
     5341 ttggctggcc tgagcagtgt attgttgagg cccatgggaa gccgcccatc cagttttagt
     5401 tcacgccttt gcagcgcctg tgagaatttt gtccgccagc acgaaggcgg cgctgaagtg
     5461 gaaatgaccc tgcttttcgc ggatgttcgg gattcaactc ctttggcgga agcgaaaggc
     5521 acactcgggt tcaaagatat catcaatcgt ttttacaagg aagcaagcga tgtgctggtc
     5581 gcacaccacg cgatggtcaa ccggttgatg ggagatcaaa ttatcgcgtt gtttgtccca
     5641 cgttttgcag gagaagctca cgcgaaggtc gcgcttcatg cgacgcagga gttattgcgg
     5701 gtgacaggtc atagaaatgc ggatggaccc tgggttcccg ttggcgcagg aatccatacg
     5761 ggcctggcat atgttggtgc ggtgggatca gccaaaggcg tcaatgagat cgcagtcctg
     5821 ggaagcgcgg cgaatatgtg tgcgcgtcta tcatcgaaag ctgcgaccgg ggagattctg
     5881 atcagtgagg atagtgtgaa atctggtaac ctggatgtga aagatttgga gagccgttcg
     5941 cttgagttga agggagtaag tcagccagtc tcagtaagag cgataaaagt ttagtaatgt
     6001 atttgaggga accaaacccg taaaacagtg ttttacagaa caatccagtc catcatacat
     6061 ttgttcagaa aaggtgactt gaaatgacaa tccttagcac gccccatcct attgaagcag
     6121 acacagaaac ccagatgaag gttcgccgtg cccgacaagg attgctcgtg tttgcatgct
     6181 tgctgatacc gctgtcactg ttcggctatt ggttttatgt gaacttcccg gatgcccctc
     6241 ttgttttgcc ttctctgccc ctgatgcttt cgcccgggct tgctgcgata ctcacgcgat
     6301 tcttaaggcg cgagggtttt gcagatgtat catttcgctt gcgaggtccc aggatcgaac
     6361 gtgcttatct gttggcgttt gcacttccgc tggttgtcgg cggagcggcg tatggcttgg
     6421 catatctcct cggtcttgcc agatttgacc cacctccgtt tccattcgcc gtcagttcac
     6481 cgattgcaca atttgcgctg aatgtaattt tctctttaat cctcatcatc ctgcttctgc
     6541 cctcagcagg aggggaagag attggttggc ggggttatct attaccccgc atgattgatt
     6601 ctggtatcca aaagcctata ttgctcacat cattgatctg gggtgcatgg catttgccag
     6661 ttgtatttgc cggtgtctat gctgttggtc catcaccgtt gctctcggca gcaggactga
     6721 tgatcgctac cctggcattt ggttcaatcc tggcctggat aaggcttgga acaggtagca
     6781 tctggccctg tatcattgcg cacgccgcct ggaatgccat tatcaatggt gcattcactc
     6841 ccgcgacaca aaacgcaact gagaacgcat gggttggaga gactggtatt cttgtggctg
     6901 tggccttagt agttgctgcg gtctggttgg gaagaagctg gggatctagg taatctgaca
     6961 gtcgcttgtt ccatagtgta tatcgatttt tcttgatctg cggcttagac aacatcatac
     7021 ggcgaaatcc tattaagaaa gtattgcaga cttctttgtc agtgataggc aggaagcata
     7081 tcaccatgtg tctcgatcag ttcatctacc attgaccaga tctgatcaag gctcagttcg
     7141 gctgccgtat gtgggtcaag catcgcggcg tgatagatat gctcacgttt ccctgtgagc
     7201 gcggcctcca caacaagaga ttgaacattg atattagttt gctgcagcgc ggcaagatga
     7261 ggcggcatca atccaatctt ggtgggttgc agtccgctct tatccaccag gcacgggact
     7321 tccacgcagc agccctgcgg caaattatcg atcaggccgt gatttggcac gttgccatat
     7381 accacgcgcg gctcacctgt ctccatcgag tgaatgatca acgaaccgta ttcatggctg
     7441 cgttcatgct ggatttcact gatttcgccg cgttccagag agtccttcac tgctttccac
     7501 ccggcaattt gcagttcgca tcgcctgata tattcgtcga gggggatttc aaattgctca
     7561 agcatgtctg ggcgatcgcg cttgatgaac caggggacat actcgctaaa gtgctcgctg
     7621 gattccgtga cgaaatatcc ggtacggcga aagacttcat aacgcacatg gtcagcgaga
     7681 ccatcagaac gacgtccata ggattttgct tccaatacct gttgaaggcg cgggtacaaa
     7741 tcctggccgt tgtgttcaaa tcgtaggtaa aaggccatgt ggttgatacc cgcgcagatg
     7801 taattaatct catcgtaggg tagccccata tcttttgcga gggcctccgc ggttccctgc
     7861 acactatgac acaaaccaac cgtttttatt ttgctggcgc ggctgatggc ccaggtgttc
     7921 atggccatgg ggttgacgta ttgaaggaac atcacatctg ggcaaagttc ttccatatct
     7981 ctgcaaatat ccagaaatac aggaatcgtc cgaagcccgc gcatgatgcc accgatgccg
     8041 agggtgtcgg caatcgtttg tcgcaagcca taccttttag ggacttcaaa gtcaacaatt
     8101 gtgccggggt catatccacc cacctggaac atgctgatgg catagtctgc gccatctaac
     8161 gcgcggcgac gatcagaggt ggcttcgatc tgcggctttg cacccaggaa gttcgctact
     8221 ttgttggcga caatttctga ggtatgtaat cgctcagggt tgatatccat gaggctgatg
     8281 gatacatcag ccagttccgg aaaactcagg atatcaccga tcaaattctt tgcaaaaacg
     8341 gtactccccg ctccaatgaa tgcgattttc gtcatgatat tctcctggtg aaatttgctt
     8401 cgagcattat agacaaattc cttccaaata aaaagagagc agagaccacg gtctctgctc
     8461 tctcgttttc catcttattt actacacagc cggtgcttcc gctgcttcgg gttccctgtc
     8521 acgctcaaga gctatttcac cctcggcatt cagaataact cgtacggtgt caccatgttg
     8581 gaattcaccg ttcaacaaac gatctgacaa gggattctcc actttctgct gaatgacacg
     8641 cttgagtgga cgtgcaccaa attctgcatc atatccctgc tctgcaagag cggaaagggc
     8701 ttctggagtg gcggtcagta cgaggttgtg ctccttaata cgctccgcaa ccttatccaa
     8761 ttccagggcg acgatgtgct gaatatcttc cttgttgagc gaacggaaga tcacgaccga
     8821 gtcaagccgg ttgatgaatt ctggtcggaa agcacgtttg agtgattcgc ttaacttctt
     8881 gcgcatatct tcataggaaa gccgctcttc agtgacttca tcacgcttga gcgcaaaacc
     8941 aagcgaagat tgtcgtttga tgacatccgc accgatattt gacgtcatca caatgatcgc
     9001 gttgcggaaa tccaccttgc gtccctttgc atcggagagg tgtccctctt ccatgatctg
     9061 caagagcata ttgtgaactt cggggtgcgc cttctcaact tcatcaaaga caacaatgga
     9121 gtatggacgg cgtcgcaatg cctcagtcag ttgacctgcc tcctcgtagc cgatatatcc
     9181 cggaggcgca ccgaccaaac gagaagcggt atgccgttcc ataaattcgg acatgtctaa
     9241 ctgaactgct gcgtcttcac tgccaaacat gaatttggca agcgtcttgg tcaactcggt
     9301 cttgcccacg cctgtaggtc caaggaaaat aaatgaacca atcggccgcc ttgggtcttt
     9361 caagccagca cgggctcgtc ggactgcacg tgagatcgcg ccgatggctt catcctgccc
     9421 gataatgttc ttttgtaatt cggcttccat gtgtagcaag cgttcagact cttcctctgc
     9481 aagctgcatc agcggaaccc cagtccacat ggagacaact tcagcgatat cttctgctga
     9541 cacaacaggg ctgcttgcac gatcccagcc tgtgcggagg cgttcgatct gctcacccag
     9601 ttcgagttcg cgatcttccc attccttcac gtcttcgctg ttgccttctt cctgagccag
     9661 cgaacggttc tgacgagccg atctcaattg accaaatagc tctttggctt ctttcgcagc
     9721 gtcactcttg tacatgcgga cgcgtgacga agactcatcg ataagatcaa tggctttgtc
     9781 ggggaggaat cgctccgtta cataccgaga ggataaatgc gccgcggctt cgagcgcttc
     9841 atcggaaatc accagatgat gatgctcttc atatgccgag cgaatacctt tgaggatttc
     9901 aatggtctct tcctcggatg gctcatccac ctgaatgggc tggaagcgcc tttccagggc
     9961 agcatcggac tcgatatatt tgcgatactc atccatcgtg gttgcgccga tcacctgcaa
    10021 ttcaccgcgg gataatgcgg gcttgaggat attcgccgca tccacagacg agccggccgc
    10081 ccctgcgcca accagcatat ggacttcgtc aataaaaaga attgcgccag actgtttgag
    10141 ttcatcaatc acgcgtttga ggcgttcctc aaactgaccg cgatacatgg tgcctgccac
    10201 cagcgaacct acatccagtt gcaataaccg tttgttcatc aacggtgcgg ggatatcacc
    10261 atccacaatt cgctgggcga gcccttcaac gatcgcagtt ttaccgacgc ccggttcccc
    10321 gatcaaagcg gggttgttct tcgtgcggcg tgcgaggatt tgaatgaccc gttcgatttc
    10381 catctgacgg ccaatgacag gatccagttt tttctcttct gcttttgttg tcaagtcaga
    10441 agcgagttga tcaatcagtg gagtttttgg attggccgcg gagctccgtg aagagcccga
    10501 accagccggt gtcggggaag acgatgtgga ttcattgagt acacggcggg tctgacggcg
    10561 gatttgttct gccgtaacgc ccagacgtcg taaaacttcc atcgccgcgc tatccacgcg
    10621 gaccaatccg agcaaaatat gctcggtacc aatataatga tgacccagcc gacgggcttc
    10681 atcgactgcg tgttcgagta cctgttgcgt atcggaggca agctccacgc gacttggatc
    10741 gaaactggtg gaactattcg tcacgcgccg gattacttcc cgtacgcggt cagacgtcat
    10801 ccctaattca cgcaggaccc gtccagctac tccaccctct tcatccatta aaccgagcaa
    10861 aaggtgctca gtacctatat tattattccg agcgcgttca gcttcttgat gtgctaggct
    10921 gagaacgcgc cttgctctct gtgtgaaacg ctccattcct gccatatttt tctcctgaat
    10981 aagattattc taccaaaaag gcgtgaaact cactgtagga aaaagattag agtttgttta
    11041 caacactgat acaatatcat aacagttctg taaaccatga cggtaatcaa agcaatatgt
    11101 atcttctgtg gatcatctga ttccgttcac gcggactatt tgactgccgc gcgttcaatg
    11161 ggtttgacac ttgccgaacg aggaatccgt ctcatttacg gaggcggaaa aacgggactg
    11221 atgggtgccg tggcaaaagg tactcttgaa gcaggcggtg aagtgattgg tgtcattatt
    11281 ccatctatga acacgatctc tcttgcatat gacggactca cccgaatgga tgttccacct
    11341 gacatgcatg cccgcaaagc ccgaatgcat gagcttgcag atgcctatat tgcccttccc
    11401 ggtggttttg gtacttttga tgagctgttt gaaaccgtta cctgggcgca aacgggcgca
    11461 catgagaaac ccgttgggtt gttaaatgta aggaattact acgctccatt actggctgcc
    11521 attgaccatg ccgtggagga agggtttgtc ttcaaagagc atcgcgattc cttgtttatc
    11581 gactctgatc ccaacacatt gatcgataaa ttgacagaat acatacatcc gcatgatgca
    11641 gtgaagagat ggatgaggga ggactaatgc atgtgcatca gggggtgacc tcagtaggta
    11701 aacctgattt tgcttcaatc agccgataga cgacaacaga tttttgctga ttgctggtag
    11761 gtaagtaacc aaccagccag cgttctgcgt ccatttcgaa cgtggcgatt tgttgaagtt
    11821 tgaaatatgg cgaatcgtcg atccccataa tatgaagaaa atcaagccag ccctctccag
    11881 caatgtcggc ttgtcccggc aggatcaggc aggtgacgcc atgttccatt aaaaagaggt
    11941 ccgtcttatt tgctatgaac cattcgtact cgtcggggtc tgttaagcct ccaatatcca
    12001 caatgggttg ctggctgaag tagcgtatgg caccaatgtc aaagaccgcg caatgatcct
    12061 tctcggggag tgaatcgcga acaaagtggg ctgtggcaat acggacctgg accatatgct
    12121 cgatattggc atcatagacc ctgttccagt acaagttgtt tgcaactgca attatcaaca
    12181 gtcctaccgt catgaaccgc gccaggtatc ctggttgtgc aagtctggaa actccaaagg
    12241 caaggatgat ccacaccaca acgtgattca atgccccata tcgacttgcc gtacccagga
    12301 tcggcataaa aagcatataa gccaggttat gcaatagaaa ccataaagca aatatcatca
    12361 gcggacgcgc gtttgacctt tccacccaac ctgcccattc acgacgtaca acgaaccaac
    12421 ggcttgccgc taataaaaga ggaaaaataa caactaacca gccaaggatt gaccaataag
    12481 acggactata actcgaaatc ccaaatccgc tgttttcagc gatataaggg gcaggtagag
    12541 actttccacc gagtgcaaac accagacgat atgccagcca ggccaggggg taaaccaatg
    12601 accgaagctg gacaaaggat gataagtacg gattctgtgc tgctacgtaa tccaagccaa
    12661 gatcaaatgt aaagcgtttt ccgatcgcgc tggtgggcat gaaatgttcg gtgcgcaggt
    12721 agagataaat gaaccaggga actgaaataa taaaaccgat cagaaacgca aaaaccaatt
    12781 cgcgccgaag agaacgctgt gcccatatgt ccaccagcgc caccgaagcc gcgagaatca
    12841 ctccctcagg tcgcaccaga atcagcagcc ccaggagaat gccaaagagt ttccattttt
    12901 gctctcgata ggcaagcaac gaaagaatcg ccaatgccag aaacagcatg gtttccatgc
    12961 cactcagaga aaaccagatc aaattcccgg agatggatag cagaacgacg gcaagtagca
    13021 ggaagggcga cttccatacg ggacgaaata gagaataaat ccctccactc acaacagcca
    13081 aaccgatcat gcccaatgct tttgccgata caaataatgg aatgcccaat aacttaaagc
    13141 ctgccagcag gaacacccaa agcgggctgg tggcgcccac gcctttttcg ttgacatcgc
    13201 taaaaaacag ctttccctcc gaaactaaat tatccgcata aacaaaatgg atataagtgt
    13261 catccatggg gaactcgatc ggtgccggca gaagaaaaac aaaagtctca accacgagga
    13321 tgcatccaat caacaggatg accacccagg aaggtggggt gaatacagcg ttgcctctat
    13381 tgtccagggt ggtaagcatt aaatacctct ataaaagttt tctttaaaaa aatgcgagtc
    13441 gaataagagt aatgtaaatt tacctggaaa acaaacgtga gttatgtgat ttgactgctt
    13501 acatagcaat tttgtttctt taaaaccaac ctgtaaatgc ccagatcaat ctctttttga
    13561 attgtttcca ttgaagatgt gcgctttgtc tggctgaagt cgatttcgtt ccgtattccg
    13621 tgatcatttg ctgatacata cgttcatgtt cgtcgaacgt tgcctgtttg gggtgcccgg
    13681 cggctaatgt acgactgact cgtcccattg aacctgccag ccaggggtca gacaggattc
    13741 gaatcatgga ctgacccatc gcataggcat cacctgcttt cgtcaagtac ccattcgccc
    13801 catcataaac gatttcgggg atgcaggtgg catcaacagc caccaggggt agtccactgg
    13861 cggcggcctc cagcaagaca atgccctggg tttcgatttc ggatgccgtt acaaacatgc
    13921 aggcaatccg gtacaactca ggtaaacctt gttgcaccga gacatagccg gtaaagtgga
    13981 ctcgtccttc catgttcaga tcacgacaga tcttcataag gtgttgtttt tgacgcccat
    14041 cgcctacgat taataagtga gcatccgtat gttgcatggc cagggcggcc gcaaggataa
    14101 catgatctac gtgcttctcg gtatccagac gtccaacatg gagcataaga ggaacgtgtg
    14161 cggggagatc ccatttctga cgtatttcta ttgtcatgtc cattgaaaat ggtgggtgaa
    14221 atacattgag atccagcccg taaccaatgg tctttgcttc aagccccgtc atctggtgga
    14281 tcaatgttga aatcgacttt gtcggtgcaa tgatggaggt aaaccttttg agaagccagc
    14341 gagcgtagga ccacaaactt ttttccacgg ctgttcgaag gaaacttgga agataggccg
    14401 cgacgaacca tggaagctga tgaaccgtta atgaactggg aacctgcgct cggcgtgcat
    14461 aggcaagacc cagcaatcct atttgcaacg gttcgtgaca atgaatgaga tctggttgaa
    14521 attcctccaa cgcccgcttt acaggatagc gtgggaacaa gaggaatcgc tgtcccacgc
    14581 gcattgggtt atacatcgat ttcaagcgca agacctgaat gttctcttga actgtgatat
    14641 atggctgatc tttgtcgctg gctgcaatca cgagaacctg atgcccgcgc attgccatgt
    14701 tgtttgccag ttgttcagcg gcaattgccg cgccgctcac cattggggga taggattgag
    14761 caaggtaggc gatacgcatt gtgactcctt ttgggcttct gcaactagtg aatagatttt
    14821 gtccatcaat tcgtcggtct gcgcgtggac ttgaactggt ttctttttag atggatctgg
    14881 ttgccaggcc gcgccaaagc gcagataaca attgcctctg aattggtagt gtcctttcct
    14941 tgcctgaccg tttctgtgaa tcttcaaggc tgctacattt tgtggatcgg cgtaaattcc
    15001 cagcggtatg attggtgcgc ccgttgcaag cgccagccgc actgtacctg tccatgcttt
    15061 gacgcgttca ccgaacggca caagctgacc ttcaggaaaa atcaagatgg tctcgccttg
    15121 tttcaggaat tcatgtgcct gttcaaacgc cttaggacga tctccagcgt tgaccggaat
    15181 ctggcctgtc cctttgagga tttctccgag cacgggaact tcaaacagat caccctgtac
    15241 gacgaagcgg ggtatgctgg aaagagccag tggcagaaaa acaatatccg ttacattggt
    15301 gtgattgatt gcgatgatct ttggtccggc tgggagtgat tccagacctt tcacttcata
    15361 tcggagccga aagattctct gatacgttcg aactatcagc atccctgccg cgttggcaag
    15421 cttctttttt atttgcggag aaataccagc cttcttgcag agcatcaaaa aaaccacgcc
    15481 tgcgcaaagg acaatcacga tcatccagaa gtgtgggggt gcctgcatcc caaatgcacc
    15541 gatccagctc atcacgatgc atgccaggcc gcgccctaac aggttggcga gtgaaaaacg
    15601 gcgatgagat attttcccga gacccgccac ataacacatt gcgtcgttag ggaggaaggg
    15661 aattacaagg gataacgtga aaaaaacaat tccctgacct tccgcagatc gatcccagcg
    15721 tgtcaataca tcctttgata tccatttttc agcaaagcca cgtccgtggt gacgagccaa
    15781 taggaaggca atttctcctc cgatcacaag gctgagccat gagagcagga agccacccca
    15841 aaagccatag agatagccgc aagccaccat cagcgcctgc ccgggaatga atgccaaaaa
    15901 gacctgtaga acgaaaagga caaataaaac cacgggtccc cagagtcccg ccttctccat
    15961 ggacgacgtc actgtttctc ggtcaccgat ccatgcccac agggaggaga ttgaatcgcg
    16021 cgatatccag aggatcaaca gtccaaaaag aaataaaatg aatttgaata aataattgtt
    16081 tttcatgatg gtctcctttg ttgccccact atcgcatctc cggactgcca attccagaga
    16141 tcgaactgcc aaaaaccgcc attaaacaaa aaaaagaccc taaaggactc aaagtccttt
    16201 agggtctctc ataaaaagta actaactcgg gtgttccatt tcaaccaacg ctcgcgccag
    16261 agaggtgatc gcagcgcgtc gggtgcggtt gaacgtacct tctgagatat acagccggct
    16321 cataacgtct cgattttgtt tgccgttcac atacgcatca tggatgatca catacggata
    16381 ccactcccgc ggcggcgggt cgcgtggcac ctcactactg ggcctcatgt gttcgagagc
    16441 ttccaacagg acggcttgta cagttttccc tcgttcgaga tgggtcttct ttcccgcggc
    16501 caggcgtgct cgcacgagtt ccatctcagc catgggcgta tctgccagat aagcataatc
    16561 aaaaagattg cgcagcgcgt tctccaccac attgaccggg atgttaaact cacgcgccgg
    16621 tggtgcaggt tgtgcatctg caagcagtgc caccttttca aggcgttcgg tattttgccg
    16681 gcttagaaat agagcctcgg caatttgatc ggatggataa agcaagcgtt ccaggtcagc
    16741 gtgcgcgtaa cggagtccat tcgtgggacg ccccagcacg agcgcgccaa tttgctcgga
    16801 ctcaatgtac agaggaataa gcagtgccgc atcctccagc ggcgcaggga attgtttcgg
    16861 tgtgagatgc agaacatcgt ctgcagtaag catgccagag ggtagttcga gacgtggatt
    16921 tttccagcga aaatctgcga cctgtcgcgc cgtcgcatct tcaaaggtaa ggacgagtgc
    16981 atacgtcgct ttgatcggta cgcacaaggc gccaaggatt tgttcgagca gtatcttcat
    17041 attctcgctt tccccggcga ggcgcctcag actatgaagg ttaaagcgta agcggcgcgt
    17101 ttctcctcga tagaagaacc agtccaaaaa aattgaagcg gcatttaaac ccgtgtgagt
    17161 caggacgcct aacagaggaa agacaatcac aatgacacgc ggtgcctgat atgcaaacac
    17221 aagaacccag gcggcaagtg tataaatgct taccatcaca atgacaccga tcaggttgta
    17281 gaagaagtcg cggtcgatgg tgcgtttttc gaccagtgcg ctgtagcgtg caactccata
    17341 tccaatcata gcgacaaaaa cagccagcac gccagacatc actaccattg gcatgggaaa
    17401 aagatccagc ccggaggaga ccagcgatac cgggccaatg agcccggtga aaaccgtcgc
    17461 tccaacaagc gtcagtaatt gtcgccgaac gatttcagtc ggagcgacat aagcagagcg
    17521 cgcaaggtta ataatgctca taatggtgag cgcaattaat gcaactccga acacagaata
    17581 gagcggtccg gcgcgcaagc tgttgagata aagagggtcg ccgccttcta cagaaaacag
    17641 aatttgtgtg aaggtctgta ctgcaatcgc ggcaatcgcg acaatatagg taaagacgac
    17701 gcgcgtccaa cgccaggggt tcattttccc gggccgcatc aaaagggtgg catgatgcca
    17761 gaaggctatc gaagggataa cagaccagcc ctgcagccag gcagtcgcct tatcctccac
    17821 ctctcccgat ggccaaaacg gaaagaggaa gcgtaatccg gccgggcgaa aggtcgggtc
    17881 gggcggcgga ttcagtgcca gcaatacatt aaaaaaaagc ccacccaaag accagagggt
    17941 gagcgcagtc agccaggctg tggggacgcg gggattacgc gtgacgagat acatccccag
    18001 ccacagggac gcgataagag ccacaaagtt aactgtgaag gtgacctggt aaagtgtcat
    18061 tgtcccacat aagaataatg attgttgcgt acactatact tattttttaa caagaggtca
    18121 atagggaaca aacaaaaacc tcttaattac ctcttcccat actttgcgcg atcttcgggg
    18181 catggacaca acgcgcgcag ataatgccag ttgaagattc catagtcatg tccatcttcc
    18241 cagacgattg tcagcgcgta ggaacccacc gcgac
//
LOCUS       DASTJP010000396         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_637440, whole genome shotgun sequence.
ACCESSION   DASTJP010000396 DASTJP010000000
VERSION     DASTJP010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637440"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFQ23_11570"
     CDS             complement(<1..1178)
                     /locus_tag="VFQ23_11570"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HET9907278.1"
                     /translation="MTTNLDRSRLEALLKQEEQTFHRNHSKSYELYQRARKSLHGGVP
                     MLWMIRWAGSFPVFVREAKGAHFTDVDGHDYIDLCLGDTGAMTGHSPDATIKGIQEQM
                     QKGITLMLPYEDVIWVGEELQRRFGLPYWQFALTATDANRFVLRMARLITGRPKILVF
                     NYCYHGSVDETLIVLDEEGTPISRPNNMGPQVNPLLTTKVIEFNDIAALETALSAQDV
                     AAVLAEPVMTNIGIIHPEPSYHEALRELTRKYGTYLIIDETHTICTSPGGYTASFGLQ
                     PDFLTIGKPLAGGVPAAVYGFTEEVSQQFLSRLAVDDADVGGIGGTLAGNALSIAAMK
                     ATLQNVLTDEFYAKSIQLQERFTAGVEAVIQEFGLPWIVKRLGNRSEYWFRPSPPRNG
                     G"
     gene            1268..2368
                     /locus_tag="VFQ23_11575"
     CDS             1268..2368
                     /locus_tag="VFQ23_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9907279.1"
                     /translation="MEEPVVGNLLNAYSTFGHFEVTAYYSICFGIIPSSLKLDLPVER
                     SKDEIDSILQSMELEQFGFTTVFKDWIPDDAKNTKPFEDLAVEYLFKSDSNRMLVHFT
                     CQVNTFDIKFLYDIDRPESEKWVLETSRKLQDKFRTTKTPKFKVLIQQDGMFTTEDVE
                     IANFEAINIQELYNDDFVEMDTIITESMQKRESGMVLLHGEPGTGKTTYIKHLICKYQ
                     NKQFIFIQNDFVKDLLKPSFISFLLQNKNAILIIEDAEKVVVSRADSSDDSVVSTILQ
                     LTDGLFSDFLNIKIICTFNTNIDHIDKALLRKGRMIAKYNFAPLAAEKASALAKKLGY
                     EHVTGSLTLADIFGMDKRGFDRSAKRTIGFSQ"
     gene            complement(2436..>3498)
                     /locus_tag="VFQ23_11580"
     CDS             complement(2436..>3498)
                     /locus_tag="VFQ23_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9907280.1"
                     /translation="QANLVIHKPMLQLIEELRTIVPPSIDSFYFANSGAEAVENAIKI
                     ARVATGRQNVIVFSGSFHGRTAGTMVLTTSKTVYRTGFGPLPSGVFVAPFPYAFRLKM
                     TEAEASEYALEQLEYLLASQTAPKETAAILVESVLGEGGYVVPPKSFIKGLREICDKH
                     KIMLIFDEVQSGFGRTGKWFALEHFEVEPDIITAAKGLASGMPLSGVFTRTDIMLKMD
                     VGSIGGTYGGNAVACAAAVASIRALREDKMIENARERGIQLMTGLRKYQEEYPEIGDV
                     RGLGLMIGTEFIVDGRADKAKQLVKDVIHSAEDKGLLLLSCGTYDNTLRWIPPLNVTS
                     EQINDGLRIFGESLKEVVK"
BASE COUNT          918 a          888 c          818 g          874 t
ORIGIN      
        1 ccaccatttc gaggagggct ggggcggaac caatattcgg accggttgcc gagtcgctta
       61 acaatccatg gcagaccaaa ttcctggatc accgcctcaa ccccggcggt gaagcgttcc
      121 tgcaactgga ttgactttgc gtaaaactcg tctgtcagca cattttgaag cgtggccttc
      181 attgctgcaa tagaaagcgc gttgccggcc aaagtcccgc cgatgccgcc tacgtccgcg
      241 tcatcaactg caagcctgga taagaattgc tggctcacct cttccgtaaa tccgtagact
      301 gctgcgggca cgcctccagc cagcggcttg ccgatggtga ggaaatcggg ctggagtccg
      361 aatgaggccg tgtatccgcc gggactggtg caaatcgtgt gggtctcatc aataatcagg
      421 tatgttccgt acttgcgagt caattcccgc aatgcctcat gataactagg ctctggatga
      481 atgataccaa tgttagtcat aacaggctcc gcgaggactg cagcaacatc ctgcgcagaa
      541 agtgctgttt caagtgcggc aatatcattg aactcgatga ctttggtagt aagaagggga
      601 tttacttgtg gtcccatatt gttcggtctt gagatgggtg ttccctcttc gtcgagaaca
      661 atcaacgttt catccactga gccatgataa caataattga aaacaagaat ctttgggcga
      721 ccggtaatca ggcgcgccat ccgcaagaca aaacgattcg catcggttgc ggtcagcgcg
      781 aactgccagt acggcagacc aaaacgacgc tgcaattcct cccccaccca aatcacatct
      841 tcatacggaa gcatgagggt gatgcctttt tgcatctgtt cctgaatccc ctttatggta
      901 gcgtccggcg aatgtcccgt catcgcgccc gtgtcaccga ggcacaggtc aatgtaatcg
      961 tggccgtcca catctgtgaa atgtgccccc ttcgcttctc ttacaaatac gggaaatgaa
     1021 ccagcccagc gaatcatcca cagcatgggt acgccgccat gcaacgattt gcgggcgcgt
     1081 tggtagagct cataggattt ggaatgattc ctatgaaatg tttgttcctc ctgtttcagg
     1141 agagcttcga gcctggatcg gtctaaatta gtggtcatgt ttcgtttaat atattgtcaa
     1201 aatgtctgaa ccgcattata gtaccatgcc atgtgggaaa aatttagtgt cactggagat
     1261 agcacaaatg gaagagcctg ttgttggaaa cctcttgaac gcttacagca ccttcggtca
     1321 ctttgaagta actgcatatt attcgatttg ttttgggatc atcccttcca gcctgaaact
     1381 tgacctgccc gtggaacgat ccaaagacga gatcgactct atcctgcaaa gcatggagct
     1441 cgagcagttt ggttttacca ccgtgttcaa ggactggatt ccggatgatg cgaagaatac
     1501 caagccattc gaagaccttg cggtggaata tcttttcaag agcgattcca accgaatgct
     1561 ggttcatttt acctgtcagg tgaacacatt cgatattaaa tttttatatg atattgatcg
     1621 gcctgaatct gagaaatggg ttctcgaaac gagtcgaaag cttcaagata aatttagaac
     1681 aacaaaaacg ccaaagttca aggtcctaat ccagcaggat gggatgttca caaccgaaga
     1741 tgtggaaatc gccaactttg aggccatcaa tattcaggaa ttatataacg acgatttcgt
     1801 tgagatggat accatcatca cagaatccat gcaaaaacga gaatccggga tggtgttact
     1861 tcatggtgaa ccagggactg gcaaaaccac ctatatcaaa cacttgattt gtaaatatca
     1921 gaacaagcaa ttcattttta ttcaaaatga cttcgtcaag gacctgctca aaccgtcctt
     1981 catttcattc cttctccaaa acaagaatgc catcttaatt attgaagatg cagagaaggt
     2041 agttgtttct cgtgcagact catccgacga ctcggtggtt tcaaccatcc ttcaactgac
     2101 cgatggactc ttcagcgact ttctcaacat caaaattatc tgtacgttca acaccaacat
     2161 tgaccatatc gacaaggcgc tcctccgaaa aggaaggatg atcgcgaaat ataactttgc
     2221 tccgctcgct gctgaaaaag cgtccgcgct ggcgaaaaaa cttggatacg aacatgtgac
     2281 aggtagcctc acgctggcag atattttcgg catggacaag cgcggcttcg ataggtctgc
     2341 aaagaggaca atcggttttt cacagtagta aaacgcacaa ataaaaaagc acggaaaaag
     2401 attcgtgtcc atttccgtgc cgatcgtttt acaaattact tcacgacttc ttttagcgat
     2461 tccccgaaga tccgcaatcc gtcattgatc tgctcggagg taacgtttaa tggcggaatc
     2521 cagcgcaggg tattgtcgta ggtcccacag gaaagtaaca ataaaccttt atcttcagca
     2581 gagtgaatta cgtccttgac aagctgtttc gctttgtctg cgcgtccatc gacaatgaac
     2641 tccgtcccga tcattagccc cagcccgcgc acatcaccaa tctccggata ttcttcctga
     2701 tatttgcgta gaccggtcat caattgaatg ccacgctctc gcgcattttc aatcatttta
     2761 tcttcgcgca atgcccgtat gctcgcgacc gctgctgcac aggcaaccgc gttgccacca
     2821 tacgttccac cgatggagcc gacatccatc ttgagcatga tatcggtgcg agtgaatact
     2881 cctgagagag gcatgccaga cgcaagtccc ttcgcggcgg tgatgatatc aggctccacc
     2941 tcgaaatgtt ccagcgcaaa ccattttccc gtgcgcccaa aaccagattg gacttcgtcg
     3001 aagatgagca tgattttgtg cttatcacaa atctcacgca agccttttat aaacgacttc
     3061 ggtgggacga catatccacc ttcgcccaaa acggattcca ctaaaatggc cgccgtctcc
     3121 ttcggcgcgg tctgtgaggc aagcaagtat tccaattgtt cgagtgcgta ctcagaagcc
     3181 tctgcctccg tcattttcag gcggaatgcg taggggaacg gcgcaacaaa cacacctgaa
     3241 ggcaatgggc caaacccggt gcgatacacc gtcttggagg ttgtaagcac cattgtcccg
     3301 gcagtgcgtc catggaagga gccgctgaaa acaatcacat tctgtctgcc ggtcgcgacc
     3361 cgcgcgatct tgatggcgtt ctcgactgct tctgcgcccg agttagcgaa atagaaggaa
     3421 tcaatggaag gcgggacgat agttcgtagt tcttcgataa gttgcagcat cggtttgtga
     3481 attacaagat tcgcctgt
//
LOCUS       DASTJP010000397         6448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_298086, whole genome shotgun sequence.
ACCESSION   DASTJP010000397 DASTJP010000000
VERSION     DASTJP010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298086"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFQ23_11585"
     CDS             complement(<1..479)
                     /locus_tag="VFQ23_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET9907281.1"
                     /translation="MKPAPFEYHAPDSIEQAVALYSQYGGEAKILAGGQSLVPAMNFR
                     VVQPGVLVDLNRVAQLSYIREEGEVIRIGSMARERHLEFDSSIATHLPLLHEAVPFIA
                     HPQIRNRGTIGGSIVHSDPAAELPVLMTALNARLKAKNASGERWIGAKDFFVGMFTTA
                     "
     gene            complement(643..1683)
                     /locus_tag="VFQ23_11590"
     CDS             complement(643..1683)
                     /locus_tag="VFQ23_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9907282.1"
                     /translation="MQTDFYDKFQELRNTGNPFAIATVIRAEKPTSAKVGAKALITAD
                     GTLSGWIGGSCAEPTVKREAKKALGDGQPRLIRLCPPEKLGTAPQEGVIEIALTCISG
                     GTLEIYIEPQLAQPHLVVIGHLATAEALVNLGKDMGWRVSLMGLDVTRERFPGLDLIV
                     DELDFSKLSIAKNSYIVVASHGNYDEDMLVAALQSEAPYVALIASKKRASAILQYLNE
                     ANLTQEQIARLKYPAGLDFGAVTPEEIALSILAEIIQRRRQTSASGGLDMSENVIRST
                     RPPSVSSLELPIAPSPATEAIDPVCGMTVEIATAHFISEYNGRTYYFCAAGCKRSFDK
                     EPGKYVQPEASL"
     gene            complement(1707..2132)
                     /locus_tag="VFQ23_11595"
     CDS             complement(1707..2132)
                     /locus_tag="VFQ23_11595"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9907283.1"
                     /translation="MSTEQNKEVVILWLEERNKGNWDIISELFAPDYVCHLASDPEPV
                     RGRDKVKMMNVGSRWGFDIYPTHEPDLLIAEADLVVHRETVRYKAKDPFRGFPPTEKE
                     AIVSIVDIYRIVDGRIVEQWNHLDRFGMMYQFGNIPTPG"
     gene            complement(2231..4579)
                     /locus_tag="VFQ23_11600"
     CDS             complement(2231..4579)
                     /locus_tag="VFQ23_11600"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HET9907284.1"
                     /translation="MTEIHGLGSSRKRVEDARFIRGQGNYIDDFKLPGMLHLDIVRSP
                     YAHAKIKKIHTEKALATPGVLAVIDGPTLQKYNVHNMPTLMSDTQMVLPIDTVKYQYQ
                     EVAGVLATDRYTAADGVAAVEVDYEALPVVVDPFKALEPGATKVRDDKESNHIWHWEW
                     GDKEKTNKDFESAEVTVKENIYIPRIHVSSIETCGCVAKWDATNGHLTLWMTTQAPHA
                     IRTVVALITGHLGLSEEKIRVISPDLGGGFGGKVPAYPGYVIAVAASIVTGAPVKWIE
                     DRTENLAADSFGRDYHITAELAGTKDGTLTSLRFKTIADHGYTDAAAQPSKMPAGLFH
                     ICTGSYDFKNAFLEVDGVYTNKPPGGIAYRCSFRVTEAVHAIERMADVYAQKIGMDPA
                     EFRMKNFIKPEQFPYTSALGWEYDSGDYHTALRKAMDMVGYEDLRKEQAEKRARGEFM
                     GIGISSFTEIVGAGPSKHFDILGLKMFDSCEIRIHPTGKAIARFGTKSQGQGHETTYA
                     QIIAEELGIPADHIKVEEGDTDTAPYGLGTYASRSTPTAGAAAAMASRKIREKSKKIA
                     AHLLEANEEDLEWERGRFFVKGSPDQGKTIQEIAFAAYTNHPQGMEGGLEAVNYYDPP
                     NLTYPFGSYICVVDIDRGTGQVNIRRFVAVDDCGVRINPMIVEGQIHGGLTMGFAPAM
                     MEEISYDENGNIHGTNFMDYLLPTAVETPKWELGETVTPSPHHPIGAKGVGESATVGA
                     PVAFVNAVVDALSHLGVTTIDIPMTPRKVWELMHKHGATLEE"
     gene            complement(4581..5066)
                     /locus_tag="VFQ23_11605"
     CDS             complement(4581..5066)
                     /locus_tag="VFQ23_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9907285.1"
                     /translation="MSTHHINVTINGKAHEADVESRLLLVHFLRENLNLTGTHIGCDT
                     TSCGACTVQLNGKPVKSCAVLAVQADGMEVKTVEGLAQNGTLHFIQEGFHEKHGLQCG
                     FCSPGMMMTSSALLEHNPHPTEDEIRHAISGNLCRCTGYMNIVKAVQYAADKMAEKVE
                     A"
     gene            complement(5068..5937)
                     /locus_tag="VFQ23_11610"
     CDS             complement(5068..5937)
                     /locus_tag="VFQ23_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET9907286.1"
                     /translation="MIPPTFDYVAPKTLSEAVAALDGNENAKVLAGGQSLIPLMRFRL
                     AGPTMLVDLTRVEGLSYIEEKDGWLRIGAMTRQSAVEHSQLVSEKYSLLADTVKVLSD
                     PVVRNMGTVGGNIANADPVNDEPAAMLAYGAEVVAVGPNGERVIPISSFYLDLYQSAL
                     ERDEILTEVRVPSPKPRSGGAYLKLERKVGDYATAAVAVQVELDEGGNFASVGIGLTN
                     VGVTAIKATAAEEFLQGKAASDENIKEAAKLAAEAAQPIADVRGSEDYKRSLVKTYTV
                     RALRLAVQRAKGG"
BASE COUNT         1469 a         1716 c         1597 g         1666 t
ORIGIN      
        1 gccgtggtga acatgcccac aaaaaagtct tttgcaccaa tccagcgttc ccccgaagca
       61 tttttggctt tcaaccgcgc gttcaaggca gtcattagta cgggtaattc ggctgccggg
      121 tcagaatgga caatgcttcc accaatggtg ccccgatttc gaatttgagg atgcgcgatg
      181 aaaggcacgg cttcatgcag caacgggaga tgcgttgcaa tggaagagtc aaattccaaa
      241 tgtcgttctc gcgccatgga accgatccga atcacctctc cctcttcgcg gatatagctc
      301 aactgtgcca ctcgattcag atcaactaac acaccgggct gaacgacgcg aaaattcatg
      361 gcaggcacca ggctttgtcc accggcgagg atcttggctt ccccaccgta ttgactgtat
      421 aaggccaccg cttgttctat ggagtcagga gcgtgatatt caaagggagc aggcttcatc
      481 aatagtcctc aaaacaagaa tcacaagctc tacaactgtt aacaccgaaa tcgcactggg
      541 gtctctgccg ccggatgcag cagtttttaa ttttatcccc tcttggtact ttggaaaatc
      601 cttctcagga tattactgca tgttgaacct acgaaaaacc cattatagag atgcttcggg
      661 ctggacgtat ttccccggct ctttatcgaa agagcgcttg catcctgccg cacagaaata
      721 gtaggtccgg ccattatact cactgatgaa atgagccgtc gcaatttcga ctgtcatacc
      781 acagacgggg tcgatggctt cggttgccgg agagggagca atgggcaatt cgaggctaga
      841 taccgatggt ggtcgagtag agcgaatgac gttttcgctc atatcgagac caccacttgc
      901 tgaagtttgt cggcgtcgtt ggataatttc agcaaggata ctcagcgcta tctcttccgg
      961 agtcacggct ccgaaatcca gccctgcagg atatttgagc cgtgcaattt gttcctgggt
     1021 caagttggct tcgtttaagt actgcaagat cgcgctggcg cgtttcttgc tggcgatcaa
     1081 cgcgacatag ggtgcttctg attgaagcgc ggcgaccagc atgtcctcat cataattacc
     1141 gtgactcgcc acaacgatat aggaattctt tgcgattgat agctttgaga aatcaagttc
     1201 atcgacgatc agatccagtc cggggaatcg ttcacgcgtc acatccagac ccataaggct
     1261 gacgcgccaa cccatgtctt tgcctaaatt gactaaggct tccgcggtgg cgagatgacc
     1321 gatcacaacc agatgaggtt gcgcgagttg aggctcaata tagatctcca acgtgcctcc
     1381 gctaatgcag gtgagtgcga tttcgatcac cccttcctgc ggagccgttc ccagtttctc
     1441 cggagggcat aagcgaatca agcgtggctg accatcgccc agcgctttct ttgcctcgcg
     1501 tttaaccgtt ggttccgcgc agcttccacc aatccaacca gagagagttc catccgcagt
     1561 aatgagagct ttcgcaccga cttttgcgga tgttggtttt tccgcgcgga taaccgtcgc
     1621 aatcgcaaag ggattgccag tatttcgtaa ttcttgaaat ttgtcgtaga aatccgtttg
     1681 cattttcttt tcgtgttgtc ttagtcttac ccaggtgtgg ggatgtttcc aaactggtac
     1741 atcataccga acctatccag gtggttccac tgttctacaa tcctgccgtc tacaatccga
     1801 taaatatcta caatcgaaac aattgcttcc ttctccgttg gagggaatcc gcgaaatggg
     1861 tctttagctt tatatcgcac cgtctcgcgg tgtaccacca agtcagcctc tgcgatgagc
     1921 aagtcaggct cgtgtgtggg gtagatatca aagccccatc tggagccaac attcatcatt
     1981 ttgactttat cgcgaccgcg gacaggttca ggatcactgg caagatggca cacgtaatcc
     2041 ggtgcgaata gttcactaat aatgtcccag tttcctttgt tgcgctcctc gagccagagt
     2101 atcacaacct ctttattttg ttcagtggac atggtttttc cttacggagg atgtccttct
     2161 gaacgaagcg aagaatctct ccttttgttt cagagaccct tcgctatcgc tcagggtgac
     2221 attactaaaa ttactcttcc agcgtcgcac cgtgcttatg catcaattcc caaaccttgc
     2281 ggggcgtcat tgggatatca atggtggtaa ctccgagatg cgagagggca tccaccacgg
     2341 cgttcacgaa ggcgaccggc gcgcccactg tagccgattc gcctacgccc ttagcgccaa
     2401 tgggatgatg cggtgatgga gtaactgtct cgccgagttc ccacttggga gtctcgacag
     2461 ccgtcggcaa cagatagtcc atgaaatttg tgccgtgaat atttccattt tcatcgtagg
     2521 atatttcttc catcatggcc ggcgcaaagc ccattgtaag tccaccgtga atttgtccct
     2581 cgacgatcat tgggttgata cgcacaccac aatcgtccac cgccacaaag cggcggatat
     2641 ttacctgacc cgtaccgcga tcaatatcca caacgcagat ataggaacca aaaggatagg
     2701 tcagattcgg tggatcgtag taattgactg cttcgagtcc accttccatg ccttgtggat
     2761 gattagtata tgcagcgaac gcaatctcct gaatcgtctt gccttgatct ggcgaaccct
     2821 ttacgaagaa acgcccacgc tcccactcaa gatcctcctc attcgcttca agcaaatgag
     2881 cggcaatctt ctttgatttt tcgcggattt tgcgtgacgc catcgcagcg gcagctccgg
     2941 cagtcggcgt actgcggctg gcataggtgc ccaagccata cggagccgta tcggtatcac
     3001 cttcttcgac tttgatgtga tcggcaggga tgccaagttc ttcagcaatg atctgggcgt
     3061 aggtggtctc gtgtccctgt ccctgtgact tggtgccgaa gcgggcaatc gccttgccag
     3121 tagggtgaat gcgaatctca caggagtcaa acatctttag tccgagaatg tcgaagtgct
     3181 tcgatggacc cgcgccaacg atttcggtga atgacgaaat gccgatgccc atgaattcac
     3241 cgcgggcgcg tttttctgcc tgctcctttc gcaagtcttc ataaccgacc atgtccatcg
     3301 ctttgcggag ggcggtatga taatcaccgc tgtcatactc ccagccgagc gcggatgtat
     3361 aggggaattg ttcaggcttg atgaagttct tcatgcgaaa ttcagcggga tccattccaa
     3421 ttttttgcgc gtacacgtct gccattcgtt caatggcgtg gacggcctcg gtcacgcgga
     3481 acgaacaacg atacgcaatg ccgcccggcg gtttgttcgt gtacacgccg tccacttcaa
     3541 ggaaagcatt cttgaagtcg tacgaccctg tgcaaatatg gaataacccg gcaggcatct
     3601 tcgatggttg ggcggcggcg tctgtatacc cgtgatctgc tatggtcttg aagcgcagcg
     3661 aggtgagcgt tccgtctttc gtgccagcca gttcagcggt gatgtgatag tcgcgtccga
     3721 atgaatccgc cgcaaggttt tcggtacggt cttcaatcca cttgacgggt gcaccagtta
     3781 cgatcgatgc cgcgaccgcg atcacatatc ccggatacgc cggaaccttg ccgccaaatc
     3841 ctccaccaag atccggagag atcactcgta ttttctcctc cgataatccg aggtgcccgg
     3901 tgatgagtgc gacgacggtg cgaatggcgt gcggcgcttg cgttgtcatc cacaacgtaa
     3961 ggtgcccatt ggtagcatcc cacttggcaa cgcagccgca ggtttcaatg gaagaaacat
     4021 ggatacgcgg gatatagatg ttttccttga ccgtcacttc cgcagattcg aaatctttgt
     4081 tcgttttttc cttatcgccc cattcccaat gccagatgtg attgctctct ttgtcatcgc
     4141 ggacctttgt cgcgccgggc tccaatgcct tgaatggatc cacgacgact gggagggctt
     4201 cgtaatccac ctcaacggca gcgactccat ccgccgcggt gtagcgatcc gtcgcaagaa
     4261 cccccgccac ttcttgatac tgatacttga ccgtatcaat cggcaaaacc atttgcgtgt
     4321 cactcatcaa tgtaggcatg ttgtggacgt tatatttttg aagcgtaggc ccatcaatga
     4381 ctgcgagaac accgggtgtt gccaatgctt tttcagtatg gatttttttg attttcgcgt
     4441 gggcatacgg gctgcggacg atatccagat gcaacatccc cggaagtttg aaatcgtcga
     4501 tgtagttgcc ttgtccacgg atgaaacgcg cgtcttcgac gcgttttctt gaagaaccca
     4561 ggccatgtat ttcagtcatg tcatgcctct actttttctg ccatcttatc ggcggcatat
     4621 tggacagctt tgacgatatt catatatccc gtgcaacggc aaagattgcc tgaaatcgcg
     4681 tggcgaattt catcttcagt tggatgtggg ttgtgttcca ataatgcaga ggaggtcatc
     4741 atcatcccgg gcgaacaaaa accacattgc aatccatgtt tctcatggaa gccttcttga
     4801 atgaaatgaa gtgtgccgtt ctgcgcaagt ccttccaccg tcttgacttc catgccatcc
     4861 gcctgaactg ccagaaccgc acacgacttg actggtttcc cattcaattg aaccgtgcag
     4921 gctccacatg aagttgtatc acaaccgatg tgtgtgcctg tcaggttgag gttctcgcgc
     4981 aggaaatgaa caagcaataa ccgagactca acatcggctt cgtgagcttt tccattgatg
     5041 gttacattga tatgatgagt gctcatttca acctcctttt gcgcgttgaa ctgcgagccg
     5101 caatgcccga actgtatacg tttttaccag cgagcgcttg tagtcttctg agccgcgcac
     5161 atccgcaata ggctgggcag cttcagcagc cagcttcgcg gcttccttga tattttcatc
     5221 tgaggcggct ttaccttgca ggaattcctc cgccgcggtg gctttgatag ccgtcacgcc
     5281 aacgttggta agtccgatgc ctacggatgc gaaattacca ccttcatcca actccacctg
     5341 tacggccaca gcggcagttg cgtaatcacc gaccttgcgc tcgagtttca gatatgcgcc
     5401 accgctgcga ggtttgggtg aggggacgcg cacctctgtg agaatttcat cacgctcaag
     5461 cgcggactga tacaggtcca gatagaacga agaaatgggg atgacacgct cgccattggg
     5521 tccgactgca acaacctccg caccataggc taacatcgcg gcgggctcgt cgttgaccgg
     5581 atcggcattc gcgatgtttc ctccgacggt gcccatattg cgcacaaccg gatcggaaag
     5641 caccttgact gtatcggcaa gcagggaata tttctcactg actaattggg aatgttcgac
     5701 agcagactgg cgcgtcatcg cgccgattct cagccagccg tctttttcct cgatatatga
     5761 caatccttcg acgcgggtta agtccactag catcgtgggt cctgccagcc taaaacgcat
     5821 aagcggaatc aggctctgcc cgcctgcaag cacttttgca ttttcattcc cgtccagggc
     5881 cgccaccgct tcggaaagtg tcttcggagc tacataatca aacgtaggtg ggatcataat
     5941 tgtctcctaa gcattcgaat ttgaagtacg gagaatcgtt cggggtagag gaaatgatga
     6001 gcagtaactc ctttcagtga gccaggttgg ggatcggact caccaaagaa aatgcgcgtc
     6061 atgatgacgc gcaaaatcag ggtgttgcga ttgatatgaa ctttctgaaa ccgaccatcc
     6121 taccgtaact gagccatcaa gcagcattga tgttctccac tatgcaaacg gcatttatga
     6181 tgatgaaatt ttcagtctga ctctctctgt aggaacgatt gtatattatt tgattgccgt
     6241 tgtttcgtca agcattacgg aaaagtttcg atgaaagcgt tgatccttaa gacgtacgta
     6301 tcttttgcag ttgtggattt gccatttcat ccacgttatt ccgcgccgcc acgatctcgg
     6361 ccataataga catggcaatc tcttcagggg tacccgcgcc aatctcaagc ccgatcgggc
     6421 cgtgcagtcg attcatctgt tcttcggt
//
LOCUS       DASTJP010000398         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1285381, whole genome shotgun sequence.
ACCESSION   DASTJP010000398 DASTJP010000000
VERSION     DASTJP010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285381"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFQ23_11615"
     CDS             complement(<1..377)
                     /locus_tag="VFQ23_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907287.1"
                     /translation="MNQLFKMAFRDLGRNKRRSILSALAVSMATTLLIFMASVVRGEF
                     RGALQNGIRLQTGHLQVRSASYDENKVSLAWEDLVDSPTQVADQLKALPQVVDATPRL
                     IASGILSLGDNSRGVQVLGIDPGA"
     gene            complement(468..1697)
                     /locus_tag="VFQ23_11620"
     CDS             complement(468..1697)
                     /locus_tag="VFQ23_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9907288.1"
                     /translation="MSLYLRLAWRNIWRHRRRTVIIVLAMGLALAMMMMYDGLVDGFN
                     NAIYGNAIRVLGGNIQVRAEGYREKVDSNPLIPLPNDEAVVKAALAHPEVTAAFRRIQ
                     TGGLISNREGAFPLSIIGIEPEAEAPVSLVAENVVDGRWLTATDEDSLLIGRGLADVM
                     DIQVGDRITMVGSDAHKQNRQRTMTVVGVYDLGMPSIEKQTVYISLAEAQSLFDLRGQ
                     STEVQINLKNLGEENIVVDSLVPALPGYEVASWAENYPELSTAITSKNFVMDIFSVII
                     QLIAGIGVLNLLLMAVFERTREIGLLGAMGLKPRQIATLFILEGTMIGIVGVVAGVLI
                     GITFNSGFGQVGFDYSSFAGVADYMALISGRVYPSLGLGKIVWRAITILIITTLAAWI
                     PAREASRREPAQALHYV"
     gene            complement(1711..2373)
                     /locus_tag="VFQ23_11625"
     CDS             complement(1711..2373)
                     /locus_tag="VFQ23_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9907289.1"
                     /translation="MPKQTFFNLPLEKRETIMNAAVEEFAEYGLENASTNRIVKNSGI
                     AKGSFYQYFEDKQDVFMHMLDVIEQKEMEFFKDRHPPSNNMDTFQYFRWMVKTGMEFG
                     LAHPRAIQAAWRVLLGEGLYYGTNLAGYREKTIKALTQMIKHAIERGEVDPSVDVELA
                     VMVMETWGNAITTYVLNEGMKQKDILKWMHSRKTQETIDKLLYVMEYGLRKTESEFTA
                     GI"
BASE COUNT          596 a          693 c          660 g          632 t
ORIGIN      
        1 gcgccaggat caatgccgag gacctgtacg cctctggaat tatcgcccag gctgaggatg
       61 ccgctggcga tcaggcgggg ggtagcgtca acgacctgcg ggagcgcttt tagctggtca
      121 gcaacctgtg tggggctatc gaccaggtct tcccacgcca ggctgacctt gttttcatca
      181 taggatgcag agcggacctg aagatgcccg gtttgtaaac gaatgccgtt ctgtaaggca
      241 ccgcgaaact caccccgcac aacagatgcc atgaagatta acagcgttgt ggccatactg
      301 accgcgagag ccgataagat cgaacgccgt ttgttgcggc cgagatcgcg gaaggccatc
      361 ttgaatagtt gattcatagt aattctcttt gtcgtttcgc ttctttcctc tttcttcttg
      421 cgtgaatttc caattgagaa aagacgaaag aagaaagaaa ggagcgatta aacataatgt
      481 aatgcctgcg cgggttcgcg gcgggaagcc tcgcgcgccg ggatccaggc agccagtgtg
      541 gtaatgatca atatggtaat agcgcgccag acgatcttgc ccaatcccag cgacgggtag
      601 acacgaccgc tgatcaatgc catatagtcg gcgacgccgg cgaacgagct gtaatcgaaa
      661 ccgacctgtc cgaaaccgct gttgaaggtg atgcctatca gtacaccagc cacaacgcct
      721 acaattccga tcatcgtgcc ttcgagaatg aacagggttg cgatttgacg cggcttgagt
      781 cccatggcac ccaacaaacc gatctcgcgc gtgcgttcaa agactgccat aagcaacagg
      841 ttcagcacgc cgataccggc gatcaactgg atgatgacac tgaaaatatc catcacgaaa
      901 ttcttggaag tgatggcggt ggacagctcc ggatagtttt ccgcccacga tgccacctca
      961 tagccaggca gggcgggaac aagtgaatcc acgacaatgt tctcctcccc gaggttcttc
     1021 aggttaatct gcacttccgt ggactggccc cgcaagtcga acaaggactg tgcttcagcc
     1081 agggaaatgt agaccgtctg tttctcgatc gacggcatgc ccagatcata aacgccaacc
     1141 acggtcatgg tgcgctggcg gttctgcttg tgagcgtcac tgccgaccat ggtgatgcgg
     1201 tcaccgacct ggatgtccat cacatccgcc aggccgcgcc cgatcaggag agaatcctcg
     1261 tccgtggcgg tcagccagcg cccatcgaca acgttttcag caaccaggct gacaggggct
     1321 tcggcttccg gttcgatgcc gatgatgctg agggggaagg cgccttcacg attgctgatc
     1381 agtccgccgg tctgtatgcg gcggaaggca gcggtcacct ccgggtgtgc cagagctgct
     1441 ttcacgacgg cttcgtcatt cggcaatggg atcagcggat tgctgtccac tttttcgcga
     1501 tagccctcag cgcggacctg gatattgccg cccagcacgc ggatggcgtt gccgtaaatt
     1561 gcattgttaa atccatccac cagcccgtca tacatcatca tcattgccag tgccagaccc
     1621 atggccagca cgatgatgac ggtgcggcgg cggtgccgcc agatattacg ccatgcgagg
     1681 cgcagatata aactcattgt gacttccttt ctaaatgcct gctgtaaatt ctgattctgt
     1741 cttacgcaaa ccgtattcca ttacatataa aagtttgtcg attgtttcct gagtcttccg
     1801 tgagtgcatc catttcagga tatctttttg tttcatgcct tcattcaaaa cgtatgtggt
     1861 gatcgcattt ccccaggttt ccatgaccat gacagcgagt tccacatcca cgcttgggtc
     1921 tacttcgcca cgttcgatag catgcttgat catttgcgta agtgctttta tggtcttttc
     1981 gcgatacccg gcaagattcg taccgtaata caaaccttcg ccaagcaata cacgccatgc
     2041 ggcttgaatg gcacgcgggt gggcgagacc aaattccatg cctgttttca ccatccagcg
     2101 gaagtactga aaggtatcca tgttattact tggtggatgc ctgtccttga agaattccat
     2161 ttctttctgc tcgatcacat cgagcatgtg catgaagaca tcctgcttgt cttcaaagta
     2221 ctggtagaaa cttcccttgg caatgccgct gtttttgacg atccggttgg tggaagcgtt
     2281 ctccaaacca tattcggcaa attcttccac tgcagcgttc atgatcgtct cacgtttttc
     2341 gagggggaga ttgaaaaaag tttgttttgg cataaaaata tgacctccag gtcatatgac
     2401 ttgcaggtca cattatagga caaattttgt ccgtgtcaat atgattatgg gtgatttaag
     2461 tcacttttcg atggatttac cgctcggtgc cagccgaaac tggttcaggt ttcaataagc
     2521 ctagtcttcc cccaagcatt ttcagcatca aacctgcccg ccaaaacgcg accgcggcca
     2581 t
//
LOCUS       DASTJP010000399        10986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1422531, whole genome shotgun sequence.
ACCESSION   DASTJP010000399 DASTJP010000000
VERSION     DASTJP010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422531"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1721)
                     /locus_tag="VFQ23_11630"
     CDS             complement(6..1721)
                     /locus_tag="VFQ23_11630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9907290.1"
                     /translation="MKSFFSNRRGLVQLFAVTVLPLSALLVIIVFWSVNMQQQNMRAL
                     VGERDQRAIQSAALALESELDKRADAISSLALLANSFSSDSLDSLIREIDDGPSANFD
                     GGIAFFDANGVLIASTDQSLLWETTSPEFATEPLDSYADGKAQFFSPITDPDQGKSFI
                     VISAFSSKSNLIVAGGISIESLAHDTLSASYPIGSNVTVYLVDSNGKLLFVNGVLGPH
                     SLTPDHPGIAEALNGETGTLFVEEGKAEHVVAFSPISTTNWGLITEEEWRAVISTSLQ
                     ATQMVPLVLAPAFILALIALWFGARQIVQPLQRLESKATSLAWGDFESIMEPVGGISE
                     VQHLQYELTEMARKVQAAQDGLHDYIAAITSAQEEERMRLARELHDDTIQAVIALKQR
                     VQLAQKSVKGSNGRHALNELETLAEQTIENLRRLTRALRPIYLEDLGLVTALDMLVHE
                     TNDHLRSPEVKFYKAGKEQRLGHDVELALYRIAQEALNNVVHHSQAKHANLSILFEGD
                     RVQLEVSDDGVGFQMPKSPTDFTPSGHFGLMGIRERADLIGARLEVKTEVGNGTRLSV
                     LLDNI"
     gene            complement(1829..2515)
                     /locus_tag="VFQ23_11635"
     CDS             complement(1829..2515)
                     /locus_tag="VFQ23_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9907291.1"
                     /translation="MIKPSKKTNTKIRIILADDHNIVRAGIRQLLESARDIEVLAEAG
                     NGEDAQVLIQTLRPDVAVIDIQMPKISGIDVTHWVREHVPEVGILILTAYNDDPYVKA
                     VIKAGANGYVLKTANPEELIQAVRDVKDGNTALAPAIARKLMLGRFQQEDKGFEELTE
                     RELEVLNLAAKGHTNKAIGIQLSISDRTVQVHLAHIFAKLRATSRTEAVMRGVALGWI
                     AQSTTPVANE"
     gene            complement(2706..4616)
                     /locus_tag="VFQ23_11640"
     CDS             complement(2706..4616)
                     /locus_tag="VFQ23_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907292.1"
                     /translation="MFKTVHFFLRVAFASVMAFSLLGAPAALAHPDDQHGPLGGHLVG
                     SGAFGNIDLVSVTELTSTPEIVADVAISPDGQWAFLANWGEPDCAGPETGGQNTPDAG
                     AWVVDISDLENPVQVGFIPAHQDSRPGEGMQVVNVTTRQFNGNILVMNNEHCGKNGKG
                     GVSLWDVTNPRVPKKLSEHFGDNANLSRGDANDIHSAFAWDAGDNAYVVIVDNFETAD
                     VDILDITNPKRPRLIRELDLDTMFPGITQVQLGLTQIFLHDVVVKNIDGDWIMLLSYW
                     DGGYVQLNVNNPANPTLVGDTDFNNPDPELFESTGASHLPEGNGHQAEFTADNQHFIG
                     TDEDFAPYGATNFAIADGPFAGAYSSVPVPGAAPIVILDDQFLNGPVVYGGYGCPASA
                     PIPSPGSISGYEASLLPGEEKIIVLQRGPTGDPSAPEGACFPGEKAHQAVLAGWEAVV
                     FVQRHGGTENPPFCGSGAFVDQVVGVCTNHEAYHNMFNTPVSFGPYPDGPAIGSVGNK
                     IEATAAFDGWGYVHLFSNTLVGGKFAELDTFAIPEAHNEAFAVGFGDLTVHEVATHPT
                     DPSRAYLSYYSGGLRALEIQGTELVEAGGYLDPQGNNFWGVEVFVRDGCTYIAGSDRD
                     SGLWIFRDNDSC"
     gene            5317..6855
                     /locus_tag="VFQ23_11645"
     CDS             5317..6855
                     /locus_tag="VFQ23_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907293.1"
                     /translation="MEIPKPKAGYKSWRPERARAGSALIWLLLLSLILMPFSATALTT
                     LEDAVSTFSYTQNLHPIGYSEREVPLDNTVPGQSQINSDLAFWGRTAYQGSYVGFRII
                     DVSDPENPVEILDYEDCSPGSTVGNQGDVIVWGNILVRSWNSNAPAGATCDGEPVSVG
                     FEGLHVFDVSNPLDPDLVASVDLAQGSHTATGVPDLANDRLLVYNSSSSGANAGIDIV
                     EVPLSAPQNAAFLRLEPAGRSCHDTGVILGDVMRAACAGGNGFTVWSLDPADGGSLED
                     PIQLYSRVVTGVTIGHSASFTWDGSMLIFGHEPGGGTQARCQATSSVVDRTLFFFDTE
                     TGTLLGSFLHPRPQTNLENCTWHNYNVVPTNKRHILVSGNYQSGVSVIDFTNPANVYE
                     VAFADPAPLVDPNPPVGIETGGDWSTYWYNGLIYESDITRGVIIWNLSDNTVAGAMRL
                     DHLNPQTQETSFPFKGTAFGRNQSPANQQGFVIFGESAATDASGGTGSDEGFSGDGDG
                     FSSP"
     gene            complement(7161..8276)
                     /gene="dnaJ"
                     /locus_tag="VFQ23_11650"
     CDS             complement(7161..8276)
                     /gene="dnaJ"
                     /locus_tag="VFQ23_11650"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9907294.1"
                     /translation="MSNDNYYETLGIGKNASDDEIKAAFRKLARQYHPDVNKEPDAEE
                     KFKEINEAYGVLSDKDKRARYDRFGKAGLGNTGGYHDYTEDFGDIFEDLLRGFGFSTG
                     RGNRRSPRRGRDLQLQVVLTFEEAVFGVEKEIEFQREETCSRCNGSGAEPGTTPIRCA
                     TCQGQGEVRQVRQTFLGQMVQSAPCPTCNGRGETIASPCKTCHGNGLERKTVKKKVQI
                     PAGVDGGTQIRLAGEGGPGVYGGPNGSLFIVLDVRSHKFFKRRENDIILNLDINVAQA
                     VLGAEVEVPTLDGDEKLKIPAGTQPGKVFNLKNKGVPFLRRSGRGEQMVIVNVALPTK
                     LTKEQRELFEKLAESLGTAVKPQEKGFLDWLNEALGG"
     gene            complement(8392..10284)
                     /gene="dnaK"
                     /locus_tag="VFQ23_11655"
     CDS             complement(8392..10284)
                     /gene="dnaK"
                     /locus_tag="VFQ23_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET9907295.1"
                     /translation="MAKIIGIDLGTTNSVAAVMQGGEPIVIPSAEGERLVPSVVAVNK
                     NNERLVGRVARNQAITNPQNTIFSIKRFMGRKSDDAEVERTRKRVPYTVTEAPNGDVR
                     VSLGEKEYSPPEVSAFILQKIKRDAEAYLGEAITQAVITVPAYFNDAQRNATKDAGKI
                     AGLEVLRIINEPTASSLAYGLDKKKNEIIAVYDLGGGTFDISILDVGDGVFQVRATSG
                     DTFLGGDDFDLRIMDYLIEQFKKDNGIDLQGDRQALQRLKEASEKAKIELSTTMQTEI
                     NLPYLTADASGPKHLVMTLSRSKLEQLTADLVDRTIAPLKQALSDAGLTAGDIHEVVM
                     VGGMTRMPAVQDRVRAFFGKEPHKGVNPDEVVAIGAAIQAGVLGGDVKDILLLDVTPL
                     SLSIETLGGVATSQIERNTTIPTRRSQVFSTAADSQTQVEIHVLQGERPMAADNKSLG
                     KFVLDGIPPAPRGMPQIEVTFDIDANGILKVTAQDKATGRSQHITITASSGLSESEVE
                     KMRKDAEAHAEEDRKRKELIEARNHADNTVYAAEKAIRDFGDKVPAEIKAEIEEKSAE
                     VKKAAEGEDVAAIKLATEALGAVIQKIGGAMYEQQPNAAPGNESGSTEPNPDAEPDVV
                     DGEVKE"
     gene            complement(10409..10960)
                     /gene="grpE"
                     /locus_tag="VFQ23_11660"
     CDS             complement(10409..10960)
                     /gene="grpE"
                     /locus_tag="VFQ23_11660"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET9907296.1"
                     /translation="MTDKKKHDHEEEMNEKQTEAVAMSENMEQLSSEIEALKRQVEES
                     QAKLAETVQGWQRATADFQNYKKRIERDNELNYASMKGEIIKKVLPALDDLERALQNR
                     PADDAWANGIELIARKLQNLLNSEGLKRIEAEGIPFDPNFHEAISHEHNEDFESGQVI
                     AVVQNGYMLGERVIRPALVRVAQ"
BASE COUNT         2631 a         2942 c         2593 g         2820 t
ORIGIN      
        1 ggcttctaga tgttatccaa cagcaccgac aaccgagttc cattgccaac ttccgttttg
       61 acctcgagac gagctccaat tagatccgct ctttctcgta tgcccatcaa gccgaaatgc
      121 ccgctgggag tgaaatcagt agggcttttc ggcatttgaa acccgactcc gtcatcactt
      181 acttccagct gaaccctgtc accttcaaag agaatagaca agtttgcatg ttttgcctga
      241 gagtgatgca cgacattgtt gagagcctcc tgggcgatcc gatacaaggc cagctccaca
      301 tcgtgaccca ggcgttgctc cttacccgcc ttgtagaact taacctctgg acttctcaaa
      361 tggtcgttcg tttcatgtac aagcatatcc aatgctgtca cgaggcctaa atcttccaga
      421 tagataggcc gcagcgcccg cgtcaggcga cggaggtttt ctatggtttg ctcggcaaga
      481 gtctcaagct cattcagggc gtgtcgtccg tttgaacctt tgaccgattt ctgtgccagc
      541 tgcacgcgtt gcttcaaggc gatgacggcc tgaatcgtat cgtcgtgcaa ttctcgggcg
      601 agccgcatcc gttcttcttc ctgcgcagag gtgattgctg ctatgtaatc atgtagacca
      661 tcctgcgcgg cctgcacttt acgcgccatt tcggttagct catattgcaa atgctgtact
      721 tctgatattc ctcctacggg ttccataatg gattcaaaat caccccacgc cagagaagtc
      781 gcttttgatt ctaaacgctg caggggctgt acgatttgtc tcgcgccaaa ccataaggca
      841 attagcgcaa ggataaatgc gggcgccaat acaaggggca ccatttgcgt ggcctgcaaa
      901 gaggtactga tcaccgctct ccattcctct tcagtaatca acccccagtt cgtcgtggag
      961 attgggctga aagcgactac atgctcggct tttccctctt ccacaaataa cgttccggtt
     1021 tcaccattga gagcttcagc gatgcccggg tggtcaggag ttagactgtg aggtcccagt
     1081 actccattga caaagagtaa tttgccattt gagtcgacta aatacactgt aacgttgctt
     1141 ccgatgggat aagaagcaga aagcgtatcg tgtgcaagac tttcgattga gattccacct
     1201 gccacaatca aattactctt agaagaaaaa gcggaaatca caataaacga cttaccctga
     1261 tcgggatccg taattggtga aaagaactga gccttcccgt ctgcatagga atccagaggt
     1321 tccgtggcga actccggaga cgtcgtttcc cacaacaggc tctgatcggt acttgcgatg
     1381 agaacaccgt ttgcatcgaa aaaagcaata ccaccgtcaa agtttgcaga cgggccgtcg
     1441 tcaatctcac gtataaggct atccaaagaa tcactggaaa aagagttcgc tagcagagca
     1501 aggctcgaga tcgcatcggc tcgtttgtca agttcagatt caagcgcgag tgccgccgat
     1561 tgtatggccc tctggtcacg ctccccaacc agagcgcgca tgttttgttg ttgcatgtta
     1621 acactccaga agacaatgat gaccagcaaa gctgagagtg gtaacacggt cactgcaaat
     1681 aactgcacca acccacgcct gttagaaaaa aaacttttca ttgatcagtc aaagaaagtt
     1741 caagtacggg aaaacaaatt tgccactaaa tgaaaacatc aatgaggacc tgttgaaatg
     1801 tttcctgatt tctgacttca atgccttctc attcattggc taccggtgtc gtactttgag
     1861 caatccatcc taacgcgacg cctcgcatga ccgcttctgt acgactcgtc gctcgtaatt
     1921 tggcgaagat gtgcgcgaga tggacctgta ccgtgcggtc actaatgctc agttgaatac
     1981 caatggcctt atttgtatgt cccttggcgg ccaggtttaa cacttccaac tcacgttccg
     2041 tcagctcctc aaacccttta tcctcctgtt gaaacctgcc cagcattagc tttctcgcaa
     2101 tcgcaggggc gagcgcggta ttgccgtctt tgacatcccg cacagcctgg ataagttcct
     2161 cggggttagc cgtcttgaga acatacccgt ttgcgccggc cttaattacc gctttcacgt
     2221 agggatcatc attatatgcg gtaaggatga gtataccaac ctcaggtacg tgttcacgga
     2281 cccaatgggt cacgtcaatg ccgctaattt ttggcatttg aatatcaatt acagcaacat
     2341 ccggtcgaag agtctggatg agcacttgcg catcttcccc gtttccggct tctgccagga
     2401 cctcaatatc ccttgcgctt tccaacagct gtcgaattcc tgcccgcaca atgttgtgat
     2461 catcagccag aataattcga atttttgtat tggttttttt tgatggcttt atcataaatc
     2521 gatttgccta tcttctggtt cagaatatca gaacctattg tattttgcca tgatgttcga
     2581 atttcagaca gtgcgcttct caaatacagt gccgtcccac acggttggtg ggacggcatg
     2641 aggagatggg atgttaagct tcttcatccc gtgtcagttg tgcgtaccaa ctgacctatc
     2701 cggtatcagc aggaatcgtt atctctgaag atccataaac cgctgtcacg atcacttcct
     2761 gcaatgtatg tacagccatc gcggacaaaa acttcgacac cccagaagtt attgccttgc
     2821 ggatccagat agccacccgc ttcgactaac tcagtcccct ggatctccag ggcacggagc
     2881 cctccagagt aatacgagag gtaagcacga gagggatccg ttggatgagt tgcgacttca
     2941 tgtaccgtta gatcaccaaa gccaactgca aaagcttcat tatgtgcttc tggtatcgca
     3001 aaggtatcaa gctcagcgaa cttgccacca actaatgtat tgctaaacag gtgcacgtag
     3061 ccccagccat cgaaggccgc ggtcgcttct atcttgttgc caactgaccc aatggcgggt
     3121 ccgtcaggat atggaccgaa gctaacaggt gtattgaaca tattatgata ggcctcatgg
     3181 ttcgtgcaca cgccaacgac ttgatccaca aatgcgccgg agccacagaa cggtggattc
     3241 tcagtaccac catgtcgctg cacaaagacg acagcttccc aaccagccag gactgcctga
     3301 tgtgccttct caccggggaa acaggcaccc tccggggcgc ttgggtcacc tgtggggccg
     3361 cgctgaagga cgatgatctt ctcttcgccc ggcagcaacg atgcctcgta gccagagatg
     3421 gaccctggtg agggaatcgg tgccgaagcg ggacaaccat agccaccata cacaaccggc
     3481 ccattcagaa actgatcatc cagaataacg atcggtgccg ctccaggaac aggcacggaa
     3541 gagtatgctc cagcaaatgg cccatctgcg atcgcaaagt tcgttgcgcc atagggtgca
     3601 aagtcttcgt cggtaccgat aaagtgttgg ttatctgctg tgaattccgc ctggtgccca
     3661 ttaccttccg gcagatggct cgcgccggtc gattcgaaca gctcggggtc cgggttattg
     3721 aagtcagtgt caccaaccag ggtcggattg gccgggttat taacgtttaa ttgaacgtaa
     3781 ccgccgtccc aatatgaaag cagcatgatc cagtcaccgt cgatgttttt aactaccaca
     3841 tcgtgcagga agatctgagt gagcccaagt tgaacttgtg tgatgcccgg gaacatcgta
     3901 tccaggtcca actctctaat gagtcgcggg cgtttcggat tggtgatatc aaggatatca
     3961 acgtcggctg tctcaaagtt gtccacgatc actacatacg cattgtcacc agcatcccat
     4021 gcaaacgcgc tgtgaatgtc gtttgcatcg ccgcgcgaga gattcgcatt gtcaccgaaa
     4081 tgctctgaaa gctttttggg gacgcgcggg ttggtaacat cccaaagtga aacaccgccc
     4141 ttgccgttct taccgcagtg ctcgttgttc atcacgagaa tattaccgtt gaactgcctg
     4201 gtggtgacgt ttaccacctg catgccctca ccggggcggc tgtcctgatg ggcaggaatg
     4261 aagccaacct gtacagggtt ttcgagatct gaaatatcta ctacccacgc gccggcgtcg
     4321 ggagtgtttt gtccgccagt ttctggtccg gcacagtcag gctctcccca gttcgcaagg
     4381 aatgcccatt ggccgtcagg tgaaatagca acatcagcaa cgatttctgg agtactcgtc
     4441 aactcggtta cgctgaccaa gtcaatattt ccaaatgctc cagaaccaac cagatgacca
     4501 cccaggggac catgctgatc atccggatgg gccaatgcgg ctggtgcacc gagcagcgag
     4561 aatgccatca ctgaagcaaa agctactcgc aagaaaaaat gcactgtctt gaacataaaa
     4621 attctcctcg taggcttaag tgtgaaatcg ccactgcgac atgggccggt gtgcagtaca
     4681 gctcaaagca agcaggaaac gaatttgccc gcgctctcct ttcgtatcgc caaaacaaaa
     4741 acgtactgtc cctaaaggta ttgagccaga ccaatctgca gcttcgcatt tgatgtcccg
     4801 aaaggcgtgc atttatccaa ttgaagggtt ggataaaagt cctgatatat acgaagctta
     4861 tatgaaaatc atagcaaacc gctcgttatt ttcatataag cataattgcc tatatctcaa
     4921 gaaatcgttc tgaatagaat tgactttgtc ggtcacagta tcggagcatc gcattacgtc
     4981 cgcaccgacc tatgagtgga gcgaaaggag gtccaatcag caaatcagtc aaacgctcat
     5041 cctggtaaac ggtaatcaca ccaacttctg aggagaaata gtgtcgtacc gtaatttcca
     5101 catcgcttgg ctgctcctac gacaaggtgg aacatacgga ccatcaaaaa ttcattaatt
     5161 cacatattta ggatcttgcg agcatctatc ggaatgtata ccgggcaaat attattacaa
     5221 ggggatagag cgccaggatc ccgagtattt cccagcgagc ctgatcatcg tgacttaatg
     5281 attattcatc agatcgacag aggaaaaatg catccattgg aaatacccaa acctaaagct
     5341 ggctacaaat catggcggcc ggagcgagcg cgtgcaggtt ccgcgctgat ctggcttttg
     5401 ctgctctcat tgatcctgat gccgttcagc gcaacggctc tgaccacact ggaggacgcg
     5461 gtaagcacct tctcttacac ccagaacctg catccaatag gttattctga gagagaagta
     5521 ccgttggaca ataccgtccc cggccaaagc caaatcaatt ctgatctggc cttctggggc
     5581 cgaacggcat accagggatc gtatgtagga ttccgaatta tcgatgtctc tgatcctgaa
     5641 aacccggttg aaattctgga ctatgaggac tgttcccctg gttcaactgt tggaaatcag
     5701 ggtgatgtca tcgtttgggg aaacatcctg gtgagatcct ggaattccaa tgcaccggct
     5761 ggtgcaacct gtgacgggga accagtctct gtcggattcg aaggcctgca tgtgttcgac
     5821 gtcagcaacc cccttgaccc tgatctagtt gcctccgttg atctcgctca gggatcacat
     5881 accgccaccg gtgtaccgga tcttgcaaat gatcggttgc tggtttacaa ctcgtcgtca
     5941 agcggcgcca acgccggtat tgacatcgtt gaagttccac tcagtgcccc tcaaaatgca
     6001 gcttttctgc gactcgagcc cgcgggacgc agttgtcatg acacaggtgt gattctgggc
     6061 gatgtcatgc gagccgcctg tgccggtgga aacggcttta ccgtgtggag cctggatcct
     6121 gcggatggtg gttcgctcga ggatcctatt caactctatt ctcgcgtggt cacaggcgtt
     6181 acgatcgggc attctgcttc ctttacctgg gatgggagta tgctcatttt cggtcatgaa
     6241 ccaggtggtg gaacgcaggc gcgctgccag gcgacaagtt ctgttgtgga tcgcaccctc
     6301 tttttcttcg atacagaaac aggaacactg ttaggcagtt tcctacaccc caggcctcaa
     6361 acaaatcttg agaactgcac gtggcacaat tataacgttg tgccaaccaa taaacgtcat
     6421 atcctggttt ccggaaatta tcagtctgga gtttctgtga ttgacttcac caaccctgcg
     6481 aatgtttacg aagttgcatt tgcagatccc gccccgcttg ttgacccgaa cccacccgta
     6541 ggaatcgaaa ccggcggaga ttggtccacg tattggtata acggactcat ctacgaatcg
     6601 gatatcactc gcggagtgat catctggaat ctaagtgaca acaccgtagc tggggcaatg
     6661 aggttggatc acctcaatcc acagacgcag gaaacgtcct tcccgtttaa gggaacagca
     6721 tttggtcgaa accaatcgcc tgccaaccag cagggctttg ttatctttgg tgaaagcgca
     6781 gcgactgatg ccagcggcgg aaccggaagc gatgaaggct tctcaggcga cggcgatggt
     6841 ttcagcagcc cctaactctg atgcattaga ttaacctggg caatcgaggt cggtgagaca
     6901 aatgccactt tgcccggcaa accaaaatac taatatccca gatcacattg gctgatctgg
     6961 gatattaatt ctaatcttgc tacggcaaac acattatttc cataaaagac ttcggttttt
     7021 acatcgctgg gttcgaggca ctcaacgggt gtgctattca agtcttctgc aaggagctct
     7081 tgagtgaaat aaattgccct tagccgtagc taagggcaat tttgtatgtt catcggcgca
     7141 cgaatttttg cttctctatt tcatccccca agggcttcat tcaaccagtc taaaaatcct
     7201 ttttcctgcg gcttgactgc cgtacccaac gactcagcca gcttttcaaa taactcacgt
     7261 tgttccttgg tcagtttggt tggcaaagcc acattgacaa tgaccatctg ttccccacgt
     7321 ccgctacggc gaagaaatgg aacgccttta tttttcagat taaaaacttt gcccggttga
     7381 gtacctgccg gaatcttcag cttctcgtcg ccatccaggg taggcacttc gacttccgcg
     7441 ccaagcacgg cttgagcgac gttgatatcg aggttcagga taatatcatt ctcgcgtcgc
     7501 ttgaaaaact tatgcgaacg gacatccaga acaataaaca aacttccatt cggtccaccg
     7561 tagactccgg gtccgccctc ccctgccagg cggatctgtg tgccaccgtc cacacccgca
     7621 ggaatctgca ctttcttttt cacagtcttt cgttcaagac cattcccatg acaggtcttg
     7681 cagggagatg caattgtttc accgcgaccg ttacaggttg ggcaaggcgc gctctgaacc
     7741 atttggccca ggaacgtctg gcgcacctgg cgaacttcac cctgtccctg acacgtagcg
     7801 cagcggatcg gcgtggtgcc cggttcagcg ccagaaccgt tacatcttga acaggtttcc
     7861 tcgcgctgga attcgatctc tttttccaca ccaaaaacgg cttcttcaaa ggtgagcaca
     7921 acttgaagct gcaagtcacg cccacggcgc ggagaacgac ggtttccacg tccagttgaa
     7981 aagccaaagc ctcttaataa atcttcaaag atatcgccaa agtcttccgt ataatcatgg
     8041 tagccgcctg tatttccgag cccggctttg ccaaaacgat cataacgagc gcgcttgtcc
     8101 ttatccgaaa gcacaccata ggcttcattg atttccttga atttttcctc ggcgtctggt
     8161 tctttgttta catccgggtg atactggcgc gcaagcttac ggaacgcagc tttaatttca
     8221 tcatccgatg cattctttcc aataccaagc gtttcgtaat aattgtcgtt gctcataata
     8281 tccaaaaggt ggtcgagtag gcggtggtcg ttcgccgtat cgagaccaca ctatcatatt
     8341 tgctagttga gtagtcacgc atgatttttg cgggactact caactagtga gttattcctt
     8401 cacttctcca tccactacat caggttcggc atccggattt ggctcggttg aacccgactc
     8461 attcccaggg gcagcgttgg gctgctgctc atacattgcg ccgccaattt tttgaatgac
     8521 tgcacccaac gcttcggttg ccaatttgat cgcggcgaca tcttcacctt cagcagcttt
     8581 tttcacctca gcagacttct cttcaatttc agccttgatc tcggcaggaa ctttatcacc
     8641 aaaatctcgt atcgcttttt ctgcagcgta aaccgtgtta tctgcgtgat tgcgtgcttc
     8701 gattaattcc ttgcgcttgc ggtcttcctc ggcatgtgct tcagcatcct tgcgcatctt
     8761 ttctacttca gattcagaaa gacccgagga ggctgtaatg gtgatgtgct gtgaacgtcc
     8821 cgttgcttta tcctgtgcgg ttacttttag aatgccattt gcatcgatat caaacgtcac
     8881 ctcgatttgt ggcatgccgc gcggcgcggg aggaattcca tcgagaacga acttgcctaa
     8941 cgacttgtta tctgctgcca tgggacgttc gccctgcaac acatggattt caacctgggt
     9001 ctggctatcc gcagcagtgg aaaagacctg actgcgccgg gtggggatcg tcgtattacg
     9061 ctcgatctgt gaggtagcaa caccgcccaa cgtctcgatc gaaagcgaaa gaggagtcac
     9121 atccaaaagg agaatgtcct ttacgtctcc acctaacaca cccgcctgga tcgctgcacc
     9181 gatcgccaca acttcatccg gattgacgcc cttgtgcggt tccttcccga aaaaggcgcg
     9241 gacacgatcc tgcactgcag gcatacgagt cattcctcct accatcacca cctcatggat
     9301 gtcgcccgcg gtcaagccag cgtcagacaa ggcttgctta aggggcgcaa tcgtccggtc
     9361 gacgaggtca gcggtcaact gttcaagctt cgagcgactc aacgtcatca ccaaatgttt
     9421 tggtccgctg gcgtctgcgg ttaaatacgg aagattgatc tcagtctgca tggtagtgga
     9481 aagctcgatc ttggcctttt cagaagcctc tttcaaacgc tgcaatgcct gacgatctcc
     9541 ctgtagatca atgccattat cttttttgaa ttgctcgatg aggtaatcca taatgcgcag
     9601 gtcgaagtca tcgccaccca ggaaggtatc accactggtg gcgcgcacct ggaagacacc
     9661 atcgcctacg tcgagaatgg aaatatcaaa cgtaccgccg cccagatcat agacggcaat
     9721 aatttcattt ttctttttat caagaccata agccaacgag gaagctgtgg gttcattgat
     9781 aatacgaaga acttccaaac ccgcgatctt gcccgcgtcc ttggtggcat tgcgctgcgc
     9841 atcgttgaaa taagcgggga ccgtgatcac ggcctgagta atcgcttcac ctaaataagc
     9901 ttcggcatcc cgtttgatct tttgcaggat gaaagccgag acttccggtg gagagtattc
     9961 cttttcccca agtgaaacac gcacatcacc atttggagcc tcagtgacgg tgtagggcac
    10021 tcgtttcctg gtacgctcca cttcggcatc gtctgatttg cgccccataa aacgcttaat
    10081 cgaaaagatc gtattctgcg ggttcgtaat tgcctggttc cgagccacac gcccgaccag
    10141 acgctcatta ttcttattga ccgccacgac ggaagggaca agccgctcgc cctctgcaga
    10201 tggaattaca atgggttcgc cgccctgcat gactgccgcg acagagttgg tcgttcctag
    10261 atcaatgccg atgattttcg ccatatttac tgttctcccg tagatgatat gtcactgcga
    10321 gggctttttt tgcccgaagc aatctcaagt taacaaggag attgcttcgt cgggacaaac
    10381 gccctcctcg caatgacatc ctatgatttt attgtgctac tcgtaccaag gcaggtcgta
    10441 tgacgcgctc gcccagcatg tatccattct gaacgaccgc gatcacttgc ccgctttcga
    10501 aatcttcgtt atgttcgtgc gaaatcgcct catggaagtt ggggtcaaat ggtatgcctt
    10561 ccgcttcgat gcgtttcaag ccttcagagt ttagtagatt ttgcagcttg cgcgcaatga
    10621 gctcgatccc gttagcccag gcgtcatctg cagggcgatt ttgtaacgcg cgctccaggt
    10681 catcgagcgc aggtaatacc ttcttgatga tctccccttt catcgaggca tagttcagct
    10741 cattatcacg ttcgatgcgc tttttgtaat tctggaagtc tgcagtagct cgctgccagc
    10801 cctgaactgt ttctgccagt ttcgcttgag attcttccac ttgacgtttt aacgcctcaa
    10861 tctcagatga tagctgctcc atgttttctg acattgcaac agcttcggtt tgtttctcat
    10921 tcatttcttc ctcgtggtca tgcttctttt tatcagtcat actcaattct caccttacaa
    10981 gtttta
//
LOCUS       DASTJP010000400         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_953674, whole genome shotgun sequence.
ACCESSION   DASTJP010000400 DASTJP010000000
VERSION     DASTJP010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953674"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFQ23_11665"
     CDS             complement(<1..584)
                     /locus_tag="VFQ23_11665"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9907297.1"
                     /translation="MARIEKTVFISYRRADVYTALAVYQDLKSKGYDVFFDYTSIPSG
                     DFEQIIVSNIKARAHFLLILTPTALDRCSEPGDWLRREIETAIDEERNIIPLFFKGFK
                     FGSPSVAEKLTGKLENLSRFNGLNVHEDYFQAAMDRLSTQFLSVPLDAILQPYSTKVR
                     EIVKEEQIAAENALQQGWEEIKEILKPAKGKSGVS"
     gene            complement(610..>2288)
                     /locus_tag="VFQ23_11670"
     CDS             complement(610..>2288)
                     /locus_tag="VFQ23_11670"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9907298.1"
                     /translation="MARIEKTIFISYRRKDISWALAVYQYLTSQKYDLFFDYTSIPSG
                     DFEQIIVSNIKARAHFVLILTPTALDRCSQPGDWLRREIETAIDEKRNIIPLLFDGFN
                     FGSPSIIEKLTGKLDAIPRYNALDIPSGYFMEAMERLRSRYLNVPLNAVIHPVPTEVR
                     KVVKEEQVAADKALEEKKEDIEELVKPAEEKLIESGKIQTRKATVSSLPFGMGKTATL
                     RPYGIGAGILLIAALGIFAINLLIQNAVEKETLMPTFTSQLILTATNASVPITQTQLN
                     STNTEMSVAPTPTPPIAAGSTMISPIGDMTLINIPAGQFTMGLTSEQVETLRPLCNSI
                     AGCEELLMLSHTYNVELSEFWIYQTEVSNGNYALCVNSGGCTEPQSQSDEYSNSNYYA
                     LSNFSNYPVVWVSWTQAKEYCNWAGGDLPSSAQWEYAARGKDGRLFPWGNNLPDSSLA
                     NVNYFYQQLKPVNDFKNFPSPFGLLNTSGNVWEWTLDWYDPNLYDPNTTLIDPLVDSD
                     QNLVNGAPAKVIRGSSAWYLSALASVAVQDWEYPNSPNFGIGFRCVVDGQ"
BASE COUNT          595 a          534 c          502 g          657 t
ORIGIN      
        1 gatacaccgg actttccttt ggctggcttc agaatttctt taatctcttc ccatccttgt
       61 tgaagagcat tctcagcagc aatctgttct tctttgacta tttctcgtac ctttgtcgag
      121 tacggctgca ggatagcgtc cagaggaaca ctcaggaact gtgtgctcaa tcgatccatc
      181 gctgcctgga aataatcctc atgtacattc aagccgttga agcggctcag attttctaat
      241 ttacctgtca atttttctgc aacagatgga gatccgaact tgaagccttt gaagaacaaa
      301 ggaataatgt tccgctcttc gtcaatggca gtttcgattt cgcgccgcag ccagtcaccc
      361 ggctcactgc aacggtccag agcagttggt gtcaatatca acaagaaatg tgctcgcgcc
      421 ttaatgttgc tcacgatgat ctgttcaaaa tccccactcg ggatacttgt gtaatcaaag
      481 aatacgtcgt accccttgct cttcaaatcc tgatacactg ccagcgcggt gtaaacgtcc
      541 gcgcggcggt aactgataaa aacagttttc tcaatacgcg ccatagactt actcccacta
      601 ctctaaactt tattgcccat ctacaacaca ccgaaaaccg ataccaaaat ttggactgtt
      661 ggggtattcc caatcctgca ccgctacact agccagtgcg ctgagatacc aggcagaact
      721 tcctctgatt accttggcgg gcgctccatt gactaaattt tgatccgaat cgaccaacgg
      781 atcgatcaaa gtcgtattgg gatcatataa atttggatca taccaatcca aggtccattc
      841 ccaaacattt cctgatgtat tgagtaagcc gaagggactt ggaaagtttt tgaaatcatt
      901 cactggtttg agttgttgat aaaaatagtt cacatttgcg agactagagt caggtaggtt
      961 atttccccag ggaaagagac gtccgtcctt tccacgcgca gcatattccc actgagcgct
     1021 ggacggcaga tctcctccag cccaattgca gtattccttt gcctgcgtcc aacttaccca
     1081 aacaacagga taattgctaa agttagaaag agcataataa ttgctgtttg aatactcgtc
     1141 actttgcgac tgaggttctg tacaacctcc cgagttaaca cataaagcgt aattgccatt
     1201 actgacttct gtttgataga tccaaaattc actgagttct acgttgtaag tatgtgaaag
     1261 catcaataat tcttcgcatc ccgctataga attacataat ggtctaagcg tttcgacttg
     1321 ctcactagtt agccccatcg taaattgacc agcgggaata tttatgagag tcatgtcgcc
     1381 tatgggtgaa atcatcgtgc ttcctgcggc tattggggga gtgggtgtgg gagcaacaga
     1441 catttccgta tttgtcgaat tcaattgtgt ttgagttatt ggcacagatg cattggtagc
     1501 cgttaggatt aattgcgaag tgaaggttgg catcagcgtt tctttttcaa cagcgttttg
     1561 aatcaacaag ttgatggcga atatcccgag cgccgcaatt aacaaaatac cagcaccaat
     1621 tccgtagggg cgcagagtag cggttttgcc catcccaaat gggagcgagg aaacggttgc
     1681 ttttctagtt tggattttcc ctgattcaat aagtttttct tcggcaggtt tgacgagttc
     1741 ttcgatatcc tctttctttt cctcaagcgc cttgtccgca gcgacttgtt cttccttcac
     1801 caccttccgt acttcggtgg gaaccggatg gataaccgca ttcaatggaa cgtttaagta
     1861 tctgctgcga agcctttcca ttgcctccat aaaataaccg gaaggaatgt cgagggcgtt
     1921 gtatcgtgga attgcatcaa gttttccagt cagtttttca atgatggatg gtgagccaaa
     1981 attgaagcca tcaaagagca gcgggatgat attacgtttt tcgtctatcg cagtctcgat
     2041 ctcacggcgc aaccagtcac caggctgact gcaacggtcc aaggctgttg gagtgaggat
     2101 gaggacaaag tgcgcacgtg ccttgatgtt gctaacaatg atctgttcaa aatctccact
     2161 tgggatgctt gtgtaatcga agaaaagatc atatttctgg cttgtcaggt attgatatac
     2221 ggcaagtgcc caggatatat ccttgcgacg ataacttatg aaaatggtct tttcaatgcg
     2281 cgccataa
//
LOCUS       DASTJP010000401         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_237676, whole genome shotgun sequence.
ACCESSION   DASTJP010000401 DASTJP010000000
VERSION     DASTJP010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237676"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFQ23_11675"
     CDS             <1..559
                     /locus_tag="VFQ23_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907299.1"
                     /translation="NLMNKNFTILSIVAVLFALAFSALVVQAQPAEKVAPDAAAYQTV
                     LGKPLNDRDVAEFIASNNCSGTNQFQLCSAAGVALQVGQGQKVENVYLYPNKTNEFSA
                     YRGALPLNLIASDTMANVEEKFGQPEVPQAPQAGWELGLPDWSGTPDHVYYWAVYKRF
                     GLTIIYNSPSATDKAATIHAILVSK"
     gene            683..1513
                     /locus_tag="VFQ23_11680"
     CDS             683..1513
                     /locus_tag="VFQ23_11680"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907300.1"
                     /translation="MNVITAEMTPEMEALKTRLKATWMSGDYGLFATYLEPGALEFLS
                     RISIEPGMRVLDVACGAGQTAIPMSRAGAKVTGVDIATNLIEQARARAQAENLDTRFE
                     EGDAEMLPFEDGSFDIVISLIGAMFAPRPDFVAAELKRVCRPGGKIIMGNWTPSGFVG
                     QMFKVHGKYVPSPAIMAPPVKWGDEETVRERLSDGISHLKLSRRSYPFRYPFSPSEVV
                     DFFRTYYGPSLKAFAALDAEKQSVMREELEQLWTEHNTATDGTTFIEPEYLEVVAIRQ
                     "
     gene            complement(1919..2353)
                     /locus_tag="VFQ23_11685"
     CDS             complement(1919..2353)
                     /locus_tag="VFQ23_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907301.1"
                     /translation="MGIFTEFISTFAAKPRSSEPEVIYLSGDGSYKMDIIGEAEHQAA
                     LEALCGPRLPEGINQVETASLLVTDNKTGPNKDAVRVEIRGSLVGYLNPEDAIHFRGY
                     LNTKGKPNAHGQCRAVIKGGWVYPDGSQGPYSVALDILALAS"
     gene            2826..>3073
                     /locus_tag="VFQ23_11690"
     CDS             2826..>3073
                     /locus_tag="VFQ23_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907302.1"
                     /translation="MLSKVVKISIVLLSLLALVAPVPAKSAAADTPPAEAVASTELST
                     ASLPIGESTSVIVKLNNIPMDGYKSAEFTCTYDGALVE"
BASE COUNT          760 a          838 c          719 g          756 t
ORIGIN      
        1 gaatctcatg aacaagaatt tcactatcct tagtatcgta gcagtacttt tcgcccttgc
       61 ctttagtgcc ctggtcgttc aggcacaacc ggcagaaaaa gtagcgccag atgcggctgc
      121 ctatcaaacg gtgcttggca agccgttgaa tgatcgagac gtggccgagt tcatcgccag
      181 caataactgc tctggcacaa accagtttca actgtgcagt gctgccggag tagccctgca
      241 agtgggtcag ggtcaaaaag tcgaaaacgt ctatctgtat cccaacaaga ccaatgagtt
      301 cagcgcctac cgaggtgcct tgccgctgaa cctgatagcc agcgacacga tggcgaatgt
      361 cgaagaaaaa tttggccagc ccgaagtgcc acaagcccca caggcaggct gggaactggg
      421 tctgcccgac tggagcggaa caccagacca tgtttactac tgggcagtat acaaacgctt
      481 tggactgacc atcatctaca actcaccgtc agccacagac aaagccgcca ccatccacgc
      541 cattttggtc agcaagtaac tcaacatgct tgcaacgctg gaacattccg caggtctttc
      601 taccgatgtc tttcaccctc atggatgact tgcggaatgt tctaccaaaa aaaattatca
      661 aatcactcaa aaggagaacc caatgaacgt tataactgct gaaatgaccc ctgaaatgga
      721 agcgctcaag acccgcctga aagccacctg gatgtctggc gattacggcc tcttcgctac
      781 gtatctggaa ccgggggcat tggaattttt atcccgcatt tccattgagc cagggatgcg
      841 cgtgctggat gtagcctgcg gcgccgggca aaccgctatc cccatgtcac gcgctggggc
      901 gaaagtcaca ggcgtagata tcgccacaaa tttgatcgaa caggcccgtg cgcgtgccca
      961 ggcggaaaac ctggatactc gcttcgaaga aggggacgcg gaaatgcttc cctttgagga
     1021 tggctcgttc gatatcgtca tcagcctgat cggcgccatg ttcgcgcctc gtcctgattt
     1081 cgtggctgca gaattgaagc gcgtctgccg gcccggtgga aagatcatta tgggcaactg
     1141 gactccttcg ggtttcgtcg gccagatgtt caaagtccac ggaaagtatg taccttcccc
     1201 cgccatcatg gctccgcctg tgaagtgggg agatgaagaa accgtccgcg agcgtttgag
     1261 tgacgggatc tcacatctga agcttagccg gcgctcgtat ccgtttcgct atcccttctc
     1321 gccctccgaa gttgtcgact ttttccgcac ctactatggg ccttccctca aagcgttcgc
     1381 cgcgttggat gccgagaagc aatccgttat gcgcgaagag ctcgaacaat tgtggacaga
     1441 gcacaacact gccaccgatg gaacaacgtt tattgaacct gaatatcttg aagtggtcgc
     1501 catccgccag tgaacatccg caccgaaaac ctcaaaagtc accccggacg attctgaata
     1561 agcattcaca ttgaacgtcc ggggttgcga caaccatgat cgttgctgtc tacctacccc
     1621 cgttcttttt aggcggagat ttaggttggc tccccgcaac gaggaaaact catccgatat
     1681 acctcgtcgt cttcaaaact aacaattttt tcgagctcac aaacattctt cagctcatca
     1741 accccataaa gatatccttt ccaccaatct gtattaaaca aacaatataa agcatgccct
     1801 ggcattctgt ctctattgaa atgccaatga tgcgatatgg ctgcaataat tagataactc
     1861 atcttaataa gaaaatcctc ctcagcttga ggaggatttc gcgttgacgt ggattctttt
     1921 aggaagctaa cgccaggata tccagcgcca cggaataggg tccctggctt ccatcaggat
     1981 agacccatcc acccttgata accgcgcgac actgaccgtg agcatttggc ttaccctttg
     2041 tattgaggta gccgcgaaag tgaatggcgt cttcagggtt gaggtacccg accaggctgc
     2101 ctcgaatttc aacacgaact gcgtccttgt tagggccggt cttattatct gttaccaaca
     2161 gcgaagccgt ttcaacctgg ttgatacctt ccggcaaacg cggtccgcac agggcttcca
     2221 gagcggcttg atgttctgcc tctccgataa tatccatttt atagctgcca tcccccgata
     2281 agtagattac ttctggctcg ctggatcgag gctttgcagc aaatgttgaa atgaactccg
     2341 taaatatacc catggaacat tcctttgcat gaagattgga actgctggca tatcgtagtt
     2401 gccagccctt tgtacccaag actacactct tagaagttgg ttactaacag ctatgttagg
     2461 gaagagatgc cacgtcatta tgcatactca ttgcacattc ttttcctttg cctctatttc
     2521 gataaagcgt atttataaga ccctccatct ctttccacca ggcgagggct cgcgctggca
     2581 tctaaacgag ccggtatagc ctgttttttt cacctttcaa ttcttttagt ggatttaccc
     2641 tgcggtcgtc agtacctcag tatgggcgta ctttagtatc ccccgcacct aacaaaaaca
     2701 tgtaggactc gactccattt tttgtttccc aatgcaggta gagcaggtaa ccttcgcccc
     2761 agtctgcatt tgagcatgcc gaatctgcga tgcttgagta gtgatatcaa aaatgaggag
     2821 tcgccatgtt atccaaagtc gtcaagatct ccatcgtgtt gctgagtttg ctggctcttg
     2881 tagcgccggt tcctgcgaaa tccgcagccg ctgacactcc gcccgcggag gcggtggctt
     2941 ccacagagct cagcaccgcc agccttccaa ttggtgaatc cacttcggtg atcgtcaaac
     3001 tgaataacat ccccatggat ggatataaga gcgcagaatt cacttgtacc tacgatggag
     3061 ccctggttga aaa
//
LOCUS       DASTJP010000402         5744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_435378, whole genome shotgun sequence.
ACCESSION   DASTJP010000402 DASTJP010000000
VERSION     DASTJP010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435378"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..2053)
                     /gene="guaA"
                     /locus_tag="VFQ23_11695"
     CDS             complement(389..2053)
                     /gene="guaA"
                     /locus_tag="VFQ23_11695"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET9907303.1"
                     /translation="MDSIAILDFGSQYAQIIARRVREAQVYCELFPWDAPMKDVLGIR
                     PKGFILSGGPKSVYEKDAPFIQEFIFQTGLPILGICYGMQALTHALKGEVSVSQTREY
                     GHAEIQSLVADSIVSSLSKVWMSHGDRITRMPEGFIALASSGNSPFAAIGDMNRKYFG
                     VQFHPEVNHTQNGSQLLRHFILDVCAVRPNWTPASIVDEAITRIRQQVGLEPVLAAVS
                     GGVDSTVAAALVHKAIGSQLIAVFVDTGLMRKDEGEQVAMAFRNNLNAELITVDASDE
                     FLSALKGITDPEQKRRIVGEKFIRIFEDQAKSLGQPRFLVQGTIYPDVVESSAPDRNK
                     AAKIKTHHNVGGLPENMQFELVEPLRYLFKDEVRAVGEELGLPRILVWRQPFPGPGLT
                     VRCLGEITRERISRLQAADAILIEELSKAGYLGRRIPSNHSNAGRTAPLTSRQTGPLQ
                     TGTTGPLTTGRTGSLTPELITPVAQAFVVLLPVRSVGVMGDQRTYQETAAIRAVTTED
                     FMTADWARLPHDLLAKVANRIVNEVDGINRVVYDITSKPPATIEWE"
     gene            complement(2065..2439)
                     /locus_tag="VFQ23_11700"
     CDS             complement(2065..2439)
                     /locus_tag="VFQ23_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET9907304.1"
                     /translation="MRNFLRHFAPLIGWIFAHMSFVIPVERLRETKTLMAFHHPRPSY
                     AFHVLLVPKKSVSSLKEFDSSDTTFLSDLYATVQSLIDEFHLVAYRLIVNGGEYQDFP
                     QLHFHLVSPSPLAPRTEGESLG"
     gene            complement(2534..3112)
                     /gene="xpt"
                     /locus_tag="VFQ23_11705"
     CDS             complement(2534..3112)
                     /gene="xpt"
                     /locus_tag="VFQ23_11705"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET9907305.1"
                     /translation="MNELEERILRDGKNLGGGILKVDSFVNHQVDPLLMDTCGKEFAR
                     RFASVQATKVLTAEISGIAPALTTAIHLGLPVVYARKTKPITMPDQVYLTLAPSHTKG
                     RMVELIVSPEYLANNEKVLIIDDFLASGQTILGLARLAEASGSRIVGIGALIEKTFEG
                     GRDALKTLGVPIEALARITSMEDDKIVFGESS"
     gene            3359..4066
                     /locus_tag="VFQ23_11710"
     CDS             3359..4066
                     /locus_tag="VFQ23_11710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET9907306.1"
                     /translation="MLEFLPVAEKLWLVLASSSPRRRQLLSLASWPFSIAVADVDESQ
                     FENEVPADYVLRLAESKARTIASQTYDHQIILAADTTVVDGDDILGKPGNSAEAVQML
                     TRLRGHAHQVYTGIALLRLSDGLLLKDLCVTDVPMRAYSDEEIQAYVQTGDPLDKAGA
                     YAIQHPHFHPVAEMHGCYASVMGLPMCHVVRLIRQMDISPNANVPVNCQLLLDYHCPV
                     SRAILNGTMSENLKGKR"
     gene            4063..>5744
                     /locus_tag="VFQ23_11715"
     CDS             4063..>5744
                     /locus_tag="VFQ23_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9907307.1"
                     /translation="MKLFRWLSGVVILTFLTSCGGGGGISIFPTETPLPQPIVTVMGA
                     PDAGAALSSYLDAFKADDYNTMYGSISKVSQDTITLEDFARRNRDALNEMSAGSFDYE
                     ILSSLVSPYSSEVAFRVTYHTALVGDVQREMIARFALENNAWKLQWDDSLILPELAGG
                     NVLRMDYSSPSRGNIYDRDGDVLVAQSDAHAFYIIPGNVTDESIDTLLSEVQRLCGNS
                     MEALAQEIFNTPAQFPIPLCEASSQESERIRSVFPSGLQWTDYNTRYYFQQGVGSNII
                     GYTTFISEEDMETYRRLGYAQDARVGADGIELWAEDYLAGERGGDLYVINPTTGQPVT
                     KIGEKEPKAADSVYLTIDDNLQFYAQQAIKGFTGAVVVLEVNTGRVLAMASSPSFDSN
                     IFSSNNPNGGRAAELVSGSLLNRAAQGQYPLGSVFKIITMAAGMESGLFEAQSTLDCQ
                     YDWTRLTDTVRHDWTWEHCQRDLQAGATECTRPDNSPSGVLTLPQGLMRSCNPFFWEI
                     GYVLDQNNRSSDIAKMARAFGLGSPTGIGQITEASGQIVDPPTVIDVVNQTIG"
BASE COUNT         1433 a         1524 c         1425 g         1362 t
ORIGIN      
        1 attgctgttg aaattcgaac cgccttgtcc gcagctcgcc aaaacaccaa agatccataa
       61 aaccctgttt ttccaaacaa tctgccaggc acgtgtgagt acttcaccga aattgaaatt
      121 catgttgcct ccactatgtt gttgccgttt gtaaagctgg tggtgtcgct gagctgcgaa
      181 tcaaacgcaa atagatgctt aaataagttg cattcataaa ggtaattgta atcccagcgc
      241 ttccccaacg tcaaaattca tcgttcatcc tcaatcacgc ttcgtctgcg ttcagacgat
      301 gaaactgtcc tcgctccgct cagcgcgaaa tcataaatcg caaatccaaa acacttgcac
      361 cgcacgcaag tgcaggtgtc gtaaatcatt actcccattc aatggtcgca ggcggtttac
      421 tggtgatatc atacactacc cgattaatcc cgtccacttc gttcactata cgatttgcca
      481 ccttcgccag caggtcgtgg ggcaggcgcg cccaatccgc agtcatgaaa tcctcagtgg
      541 tcacagctcg aatcgcagcg gtttcctgat acgtccgctg atcgcccatc acccccaccg
      601 atcgaacagg gagcaatacg acaaacgcct gcgctacagg agtgatcagc tctggggtaa
      661 gagacccggt gcggcccgtt gttaatggac cggtcgtccc agtctgtaaa ggtcctgttt
      721 gcctgctggt tagaggggcc gtcctgcctg cgttggaatg attggatgga atgcgccggc
      781 ccaaatagcc cgctttcgat agttcttcga tcagaattgc atccgctgcc tgtaaacggg
      841 atatgcgttc gcgcgtgatc tccccaagac agcgcaccgt cagacccgga cccggaaacg
      901 gctgccgcca aaccaggatg cgcggcaacc ccagctcttc tccaaccgcc cgcacttcat
      961 ccttgaataa ataccttaac ggctccacga gctcgaattg catattctca gggaggccgc
     1021 caacattgtg gtgagttttt attttcgccg ctttatttcg gtccggtgcg gaagactcaa
     1081 ccacatcggg ataaatcgtc ccctgcacta aaaaccttgg ctgtcccagg ctttttgcct
     1141 gatcttcaaa gatgcggata aatttctcac cgacaatcct gcgcttttgt tcagggtccg
     1201 tgatgccttt gagggcggat aaaaattcgt cgcttgcatc caccgtgatc aattcggcat
     1261 tcaagttatt tcgaaacgcc atcgcaactt gttccccttc atccttccgc atcaggcctg
     1321 tgtccacgaa cacagcgatg agttgactcc cgatcgcctt atggacaagc gccgccgcta
     1381 cggttgaatc gacgccgccc gataccgcgg caaggacggg ttcgagccct acctgctgac
     1441 gaatacgggt aatggcttcg tcaacaatgg aggctggggt ccaattcggt cgtacggcgc
     1501 aaacatccag gatgaagtgc cgaagcagtt gcgagccatt ctgtgtatga tttacttccg
     1561 ggtgaaactg tacgccaaaa tattttcggt tcatatcgcc gatcgcggca aagggactat
     1621 tcccactgct ggctaacgcg atgaaccctt ctggcattcg ggtaatgcgg tcaccgtgag
     1681 acatccagac tttagatagt gaggaaacaa ttgagtcggc gacaagggac tgaatttcgg
     1741 catgaccata ttcacgtgtt tgtgaaacgg agacttcacc ttttagggca tgcgtgagag
     1801 cctgcatgcc ataacaaatg cccagaatgg gcaacccggt ttgaaagata aactcctgga
     1861 tgaagggtgc atccttttcg taaaccgatt tcggtccgcc tgaaaggata aagccctttg
     1921 gacggatacc aagaacatcc ttcattggcg cgtcccaggg aaacaactcg caatagacct
     1981 gtgcttcacg cacacgtcgc gcgatgatct gtgcatattg tgaaccgaaa tcgaggatgg
     2041 cgatggagtc cataaagtaa tccctcaccc caaactctct ccctcagtgc gaggggcaag
     2101 aggtgaggga gaaacaagat gaaaatgcag ctgtggaaaa tcctgatact ctccaccatt
     2161 gacgatcagg cggtacgcga ccagatgaaa ctcatcaatc agactctgta cggtagcgta
     2221 tagatcagag agaaacgtcg tatcgctgga gtcaaattcc ttaagagaag aaaccgattt
     2281 ttttggcaca agcaatacat gaaaggcata ggatggcctg gggtgatgga atgccatcaa
     2341 cgtcttcgtt tcccgcaatc gttcaacagg aatgacaaaa ctcatatgtg caaagatcca
     2401 tccaatgagt ggtgcaaaat gccgaagaaa gtttcgcatg ataactcccc ctctcccgcc
     2461 gggagactgc gacacttgta cctgcgtgca agtgcaggtg agcgctccgc agaagaggat
     2521 agggcgacac taattacgac gattccccaa acacaatttt gtcatcttcc atggaagtga
     2581 tgcgcgccag ggcttcaatc ggcacgccta aggtcttcaa tgcatcacgt ccaccttcaa
     2641 aggtcttctc gatcagggcg ccaatgccaa cgatcctcga accagatgct tcggcgagtc
     2701 gcgcaaggcc taagatcgtt tgtccactgg ccaggaagtc atcaatgatc aacacctttt
     2761 cattgtttgc cagatactcc ggcgagacga tcaactcgac catgcgcccc ttcgtgtgcg
     2821 acggggcaag ggtcaaatag acctggtcgg gcatggtaat cggctttgtc ttgcgtgcat
     2881 acaccaccgg aagaccaagg tgaatcgcag ttgtcagggc aggtgcaatg cctgagattt
     2941 cagcagtcag caccttggtt gcctgaaccg aagcgaatcg acgcgcgaac tcttttccgc
     3001 aggtatccat caacagtgga tccacttgat gattcacaaa actatccact ttcaaaattc
     3061 ccccacccag attcttccca tcgcgcaaaa ttcgctcttc cagttcgttc acacgcgcct
     3121 ccatgaccag acaaatctat cgcattttac cattcccacc catcgcattg ggcacctccc
     3181 ccaaattggg agtggctaaa agagtgagtg aaaatctacc tagccacaga gagcactgag
     3241 tcctttgaga aaaaaactca aaactctgta ggctcggtgg cttacacact aaattcgcca
     3301 ctccccccaa aattctgcct gaagttcgtc ggaatttgga ggagggctgg ggaggggtat
     3361 gttagaattt ctccccgtgg ctgaaaaact ctggctggtg cttgcatcca gttcgccgcg
     3421 gcggcggcag ttgctgtccc ttgcgagctg gcccttcagc atcgcagtcg cggatgtgga
     3481 tgaaagtcag tttgagaacg aagtgccggc cgattacgtc ttaaggcttg cagaatcaaa
     3541 agcgcgtact atcgcaagcc aaacttatga tcatcaaatc attctcgcgg cggataccac
     3601 cgttgtggat ggtgatgaca ttcttggtaa accaggtaat agtgctgaag cagttcaaat
     3661 gctgacacgc ctgcgtggtc acgcacatca ggtctacacc ggaattgcgc ttctcaggct
     3721 gagcgatgga cttctgctca aggatctctg cgttacagat gtgcccatgc gcgcttattc
     3781 tgatgaagag attcaagcct acgtccagac tggtgaccct ctcgacaagg ccggcgcata
     3841 cgccattcag catccccact ttcaccctgt ggcagagatg cacggatgtt atgcaagcgt
     3901 catgggtctt ccgatgtgcc atgttgtccg cctgataagg caaatggata tatcgccaaa
     3961 cgcgaatgtg ccagtaaatt gtcagctgct attggactat cattgccctg tttcgagagc
     4021 gatcttgaat ggcacaatgt cagaaaattt gaaaggtaaa cgatgaaatt atttcggtgg
     4081 ctaagcggtg ttgtgatcct gacatttcta acctcatgtg gaggtggtgg aggaatttcg
     4141 atcttcccca cagagactcc attgccccaa ccgattgtaa ccgtcatggg cgcgccagat
     4201 gcaggtgccg cgttgtcgtc ttatcttgat gcttttaaag cggatgacta caacaccatg
     4261 tacggctcga tcagcaaggt cagtcaggat accatcacac tggaagattt cgccaggaga
     4321 aaccgggatg cgctgaacga gatgagcgct ggttcctttg actacgaaat tctttcctcc
     4381 ttagtcagcc cttattcttc agaagttgcc tttcgggtta cttatcacac tgcactggtc
     4441 ggggacgtcc agcgcgagat gattgctcgc ttcgcattgg aaaacaatgc ctggaagctt
     4501 caatgggatg attcgcttat ccttccagag ctggcggggg gcaatgttct gcgaatggat
     4561 tacagctctc catcacgcgg caatatttac gatcgtgacg gcgatgtgct ggtcgctcaa
     4621 tctgatgccc acgcctttta tatcatccca ggcaatgtga cagacgaaag catagacaca
     4681 cttctttcag aagtccagag actgtgtggc aacagcatgg aagcgctggc gcaggaaatc
     4741 ttcaatacgc cggcacagtt cccgatacca ctttgcgaag catcctcaca ggaatcagaa
     4801 cgcatccgat ccgtctttcc aagtggttta cagtggacag attacaacac ccgctattat
     4861 tttcaacagg gggttggttc taacattatt ggctatacca cattcattag tgaagaagac
     4921 atggaaacct atcgccgtct tggttatgcc caggacgccc gtgtgggtgc ggatggaata
     4981 gaactctggg cagaggatta cctcgcggga gagcgcggcg gcgacttata tgtgatcaac
     5041 ccgaccaccg gccaacccgt gacaaagatc ggtgaaaaag agccaaaagc ggctgactca
     5101 gtgtatctga ccatcgatga taatttacag ttttatgctc agcaggctat taaagggttt
     5161 acaggtgccg ttgtggtgct tgaagtcaat accgggcgtg tgcttgcgat ggcgtcttca
     5221 cctagttttg attcgaatat attctcgtcc aataacccaa atggaggccg ggccgcagaa
     5281 cttgtatcgg gatccctcct caaccgcgcc gcacagggac aatatccgct tggctctgtg
     5341 ttcaagatca tcaccatggc ggctggcatg gaaagcggct tgtttgaggc ccagtcaaca
     5401 ttggactgtc aatacgattg gacccgactc actgataccg tgcgacatga ctggacgtgg
     5461 gagcattgtc agagagattt acaagcaggt gcaactgaat gcacaagacc cgataacagc
     5521 ccatccggcg tgctaaccct tcctcaagga ttgatgcgtt cctgtaaccc gttcttttgg
     5581 gagatcggtt atgtgctgga tcaaaacaac cgcagtagtg acattgcgaa aatggcacgc
     5641 gccttcgggc tgggcagccc gacaggcatc ggacagatca cggaggcttc cggtcagatc
     5701 gttgatcccc caactgtcat tgatgtagtt aaccagacca tcgg
//
LOCUS       DASTJP010000403         7575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_650035, whole genome shotgun sequence.
ACCESSION   DASTJP010000403 DASTJP010000000
VERSION     DASTJP010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650035"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFQ23_11720"
     CDS             <1..363
                     /locus_tag="VFQ23_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET9907308.1"
                     /translation="SMRIHEMREMGIEVMILSSEPNRVVAARAKKMGVEAIHGVGIHD
                     KGRVMREILEAKHVHAENVIYIGNDRNDLPCFEIAGWSVAVADAYPEVIHAADHVLTK
                     PGGHGALRELCDLILSKV"
     gene            382..744
                     /locus_tag="VFQ23_11725"
     CDS             382..744
                     /locus_tag="VFQ23_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907309.1"
                     /translation="MNCFYHPRVIAVATCKFCQKGLCMDCAVDLEHGVACQQHQAEVI
                     ELYDIHFMHKQSAQNMSRIYKQSAVAMSFMGIAGVLGGLVIGRTGIILVAIGIGCIFL
                     AIAFSMYANRMQKEKIKN"
     gene            777..1658
                     /locus_tag="VFQ23_11730"
     CDS             777..1658
                     /locus_tag="VFQ23_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HET9907310.1"
                     /translation="MSREIRIGNRLVGDGHPTYVIAEIGINHNGDLNIAKRMIDAAVH
                     AGADAVKFQKRTPNVATPPEQQHQMRETPWGYITYLEYRYKVEFSEEQYAEIDSYCRQ
                     RAIPWLVSVWDEPSVDFMEKFDTPAYKIPSASLTDFNLIRKARATGKPLILSSGMSTM
                     EQIKMGVAIAGEENLILMHCTSTYPCEPEELNLKLVETLRQEFPRVPIGYSGHEVGLV
                     PSAVAVAFGACMVERHLTLDRAMWGSDQAASVEPGGFERLVKYIRVTESSLGDGVKKV
                     YDSEKSSLRKLRRVAGN"
     gene            1715..2569
                     /locus_tag="VFQ23_11735"
     CDS             1715..2569
                     /locus_tag="VFQ23_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9907311.1"
                     /translation="MRIDQRIRRTLRPAGKTALAILRWKRLTFNDAPPIFGNSKPKSG
                     SHLLIQILAGLCKIAPYKYVTADPVRTITKGGGRNEKEKILHELQGISRGVIGWGYVE
                     ATPENVTFLCQPGRVNYFVYRDPRDLLVSQVFFATNMYEDHGMHEFYKSLPDVGARLK
                     VAITGIRENGLHMVSVKQRYEGVFQWLEQKQVMCIRFEDLIDNRDATLNAMLDEVEKT
                     GYQIPMPRETALSILVDAIQPAKSHTFRSGRTGGWKTYFHDEHKKLFKDVAGDLLVRL
                     GYEKDNDW"
     gene            2692..3630
                     /locus_tag="VFQ23_11740"
     CDS             2692..3630
                     /locus_tag="VFQ23_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9907312.1"
                     /translation="MPVHGTHNAVQDDRNESILIYVNGELFPRNEAKISVFDSGYLVG
                     DGIWEALRLHEDVLVFLDEHLNRLWQAAATVGMDLKMTREELTDKLWQTLRANDMHDG
                     VHVRFMVTRGTKKTPSQDPRMVLSGPNLVIIPEYKQASSDTRNRGVTLFTSTIRRGSP
                     DYLDPRLNCHSKLHEVIALVQAIEAGADEALMLDIHGFVATCNATNFFMVKNGEVWTS
                     TGQYCMNGITRGKIIKVSVDNGIPCYQKNFSLFDVYGAEEAFVTGTFGGVTPVVKVDG
                     RVVGDGKPGAMSEKLSALYEAEVHRVVLAAKGAKQL"
     gene            3627..4361
                     /locus_tag="VFQ23_11745"
     CDS             3627..4361
                     /locus_tag="VFQ23_11745"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907313.1"
                     /translation="MSPDITRICLWSGPRNVSTALMYSFAQRDDTSVFDEPLYAHYLS
                     KTPARAYHPGAEEVIATMENDGEKVVDDLILGDHDTPVVFFKHMTHHLYELDLDFLDH
                     TVNILLTRDPVEMLPSFAAWVEAPSLRDVGYALHLDLLHHLQSRGQNPPVLDSKQILL
                     NPRKVLNELCERIGIPFHETMLSWQAGPRPEDGSWAKYWYKSVHESTGFGEYVKKSAP
                     FPKHLKPLLGECIPYYEKLHEVAIQA"
     gene            4548..5423
                     /locus_tag="VFQ23_11750"
     CDS             4548..5423
                     /locus_tag="VFQ23_11750"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HET9907314.1"
                     /translation="MSRLTRLWKKWITPTQITIFSLLSMLSLIFPSVSFAMQSQTASG
                     ETIYAIVERNRIIQFSSGNSCTVTSSVRIKGLQPGERLEGIDFRPLTGQLYGLGSKSR
                     LYTIDLASGVATQVGAPFSTPLNGTLFGFDFNPTVDRIRVVSDTGQNLRLHPDTGAVA
                     AVDFSLAYASTDVNAGASLFVNGAAYTNPDNDPTTGTTLYDIDTSLDILASQVPPNDG
                     VLNTIGTLGLSVNEPAGFDISLSGMAYAALQTSDTSGKCAPTKLFTIDLTTGTATLIG
                     GIGVSEHILGIAVAP"
     gene            5650..>7575
                     /locus_tag="VFQ23_11755"
     CDS             5650..>7575
                     /locus_tag="VFQ23_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9907315.1"
                     /translation="MVSSSSKPDLIAPTRIRLWVSAFRYLAGKALTILVTIFMGVFIT
                     MLIVNYPPGRGAEPTKSPFQVRLESEIDRFVQSMLYSGTIPFQGPADFEAQYESLSNQ
                     LRAEAGLDLPFWPRNLLWTFNALTFNWGELDSTFIQSLGTGGGFETDVYGNAVLHFLP
                     NTLLLVGISYLLVFLLGMPLALYLARNYGSRLDRLVTILSPISSVPSWVFAVLLIALF
                     AVQLRWLPIGGMSDIFYTPKTPIENALTVLKHMVLPVTALVLSLLFQLVYAWRTFFII
                     YSEEDYVELARAKGLDNKTLERQYILRPALPYIITSFTTSLIGFWQLTVALESVFRWP
                     GIGLLYIETLPNYWGERVAIGDLMIVIQIVVVFAYLLGMLVLILDIVYVLIDPRLHLT
                     PKNTSSLNNARVKTKATNPKRLFSTARIKDRNSDRASRVKVPGERWVFSPSRTIRAAF
                     DSIREMQSRSRLFFQELRRYPSAIFGLTIILILLAGSLYAVVALPYEEFGRSYNEDRV
                     RGFNLRPRVAAPNWFNLFSPTPRLSTLILKEDNAGASVSTSALDNGWTQKTVTFTFEY
                     PYGEMPSDVFLYLDPTYVEKFPFVSMVWKTPDGRALNLKPVGVGNAINYDFKDGVQVP
                     KFLNQNPAWKDWFAAEGQ"
BASE COUNT         1905 a         1772 c         1952 g         1946 t
ORIGIN      
        1 agtatgcgca tccacgaaat gcgtgagatg ggaatcgaag tgatgatcct ttcctcggag
       61 ccaaatcggg ttgtggcggc gcgcgcgaaa aaaatgggtg tggaagccat tcatggggtt
      121 ggcattcacg ataagggtcg cgtgatgcgc gaaatccttg aagcgaaaca tgttcacgca
      181 gagaatgtca tttatatcgg caacgatcgg aatgaccttc cctgctttga aatcgcaggc
      241 tggtcggtgg ctgtggcaga tgcatacccc gaagtgatcc atgcagcaga ccacgtcctc
      301 acaaagccag gcgggcacgg cgcgttgaga gaattatgtg atttaatatt gagcaaagtt
      361 tgaagataag aggcctaaga aatgaattgt ttttatcatc cgcgcgtgat cgcggttgca
      421 acgtgcaaat tctgccaaaa gggattatgc atggattgcg cggtagacct cgagcatggg
      481 gtggcgtgcc agcaacatca agctgaagta attgagctct acgatattca tttcatgcac
      541 aaacaatcgg ctcaaaacat gagccgaatc tacaaacaat ctgccgtagc catgagcttt
      601 atgggaattg ctggcgtcct gggcggactg gtgattggca gaacaggcat tatcctggtg
      661 gccattggga ttggctgtat tttcctcgcc atcgcttttt cgatgtatgc caatcgcatg
      721 caaaaagaaa agataaagaa ttagctttat atttctcaat gtataaggaa tagacaatgt
      781 cgcgtgagat aagaattgga aaccgtttgg tgggtgatgg acatcctacc tatgtgattg
      841 ctgagatcgg cataaatcat aacggtgacc tgaatattgc aaaacgtatg atcgatgcag
      901 ctgtgcatgc gggcgcggac gcagtgaagt tccagaaacg cacacctaat gtcgcaaccc
      961 cgcctgaaca acagcatcag atgcgtgaga caccctgggg ctatattaca taccttgaat
     1021 atcgttacaa agtggaattc agtgaagaac aatatgccga gatcgattcc tactgccgtc
     1081 agagggcgat cccatggctg gtctctgtat gggatgagcc ttcggtggat tttatggaga
     1141 agttcgatac gcccgcctat aagattccat ccgcctctct tacggacttc aacttgatcc
     1201 gcaaagcccg ggcgacgggc aaacccctga ttctttcttc ggggatgtcc accatggagc
     1261 agatcaaaat gggtgttgcc attgcaggag aagaaaattt gatcctgatg cattgcacca
     1321 gtacctatcc ctgtgagcca gaagagctaa atttgaagtt ggtggaaacc ttacgtcagg
     1381 aattcccaag ggtgcccatc ggatactctg gccacgaagt agggcttgtg ccatccgcag
     1441 tcgccgtcgc ctttggggca tgtatggtgg agcgccatct cacgctcgac cgcgccatgt
     1501 ggggtagcga ccaggccgcc tcggtggaac cgggcggttt cgaacgattg gtgaagtaca
     1561 tccgtgtgac tgagtcctca ttgggcgatg gtgtgaagaa agtgtatgat agcgaaaaat
     1621 cttcactcag gaaattgcgc cgcgtggcag gcaattaact ttcaccgcga agagcgccaa
     1681 gcacgcgaag atgtgcttgg cagttcaaga tattatgaga atcgaccaac gaatccgccg
     1741 cacgctacgc cctgccggga aaaccgcttt agccattttg cgatggaagc ggctgacctt
     1801 caacgacgcg ccacccatct ttggcaactc caagcccaaa agcggatcgc acttgctaat
     1861 acaaatcctc gccgggttat gtaagatcgc accttataag tacgtgactg cagacccagt
     1921 gcgcaccata accaaagggg gaggaagaaa tgaaaaagag aaaattctcc atgaattgca
     1981 ggggatttca cgtggcgtga ttggctgggg ctatgtggaa gcaacgccgg agaacgtcac
     2041 gtttttatgt cagcccgggc gcgtcaatta ttttgtctat cgcgatccac gcgatctgtt
     2101 ggtctcgcag gtattttttg caaccaatat gtatgaagac catggcatgc acgaatttta
     2161 caaatcgctg ccagacgtgg gagcaagact caaagtagca attacgggca tccgtgaaaa
     2221 tggattgcac atggtcagtg tgaagcagag atacgaaggt gtattccaat ggctggaaca
     2281 gaagcaagtg atgtgcatcc gttttgaaga tttaatagac aatcgcgatg ccaccttgaa
     2341 tgccatgctt gatgaagtgg aaaagacggg gtatcaaatc cctatgccgc gagagacggc
     2401 attatccatc ctggtagatg cgattcagcc ggcaaagagt cacacttttc gttcggggag
     2461 gacgggagga tggaaaacat atttccacga tgagcacaaa aagctgttca aggatgtggc
     2521 aggagatttg ctggtacggc tggggtatga gaaggataat gattggtaag tggagacagt
     2581 aggggtttaa ttaggctctg taattcattc acaacgatct tgtcttaacg aaattacctt
     2641 gctgaatttc caaatcccaa ttctcagact tcacaaattt tggagtagac aatgccagta
     2701 catggaaccc ataacgcagt acaggatgat cgtaacgaat cgattctaat ttatgtaaat
     2761 ggcgaactct tccctcgcaa cgaagcaaag atatcagtct ttgatagcgg ctatcttgtc
     2821 ggcgatggga tttgggaggc actccgtttg catgaggatg tgctggtctt cctggatgaa
     2881 catctcaatc gcttatggca agcggctgct acagtcggca tggatctcaa gatgacgcgc
     2941 gaagagctaa cggacaaact ctggcaaact ctgcgtgcta atgatatgca cgatggtgtg
     3001 cacgtccggt ttatggtgac gcgcgggacc aaaaagactc catctcagga tccgcgtatg
     3061 gtgttgagcg gacccaacct ggtcattatc cctgaatata aacaggctag ttccgacact
     3121 cgaaatcgtg gtgtgacttt gttcaccagc acgatccggc gcggctcgcc agattatctc
     3181 gacccgcgtc tgaattgtca cagcaagctg cacgaagtga tcgctttggt acaagccatc
     3241 gaagcaggcg cagacgaagc cttgatgctg gatatccatg ggtttgtcgc cacgtgcaat
     3301 gccacaaatt ttttcatggt gaaaaatggc gaagtgtgga cctccaccgg gcaatattgc
     3361 atgaacggga tcacacgcgg aaaaatcatc aaagtctccg ttgacaatgg cattccctgt
     3421 tatcaaaaga acttttcgct attcgatgtg tatggcgcgg aggaagcctt tgtcacaggc
     3481 acgtttggtg gcgtgactcc cgtagtcaaa gtggatgggc gagtggtcgg cgatggaaag
     3541 cccggggcga tgagtgagaa actctcggca ttgtatgagg ctgaagttca ccgggtagtt
     3601 ctggcggcga aaggggcgaa gcagttgtga gtccagatat cacgcgcata tgtttgtgga
     3661 gtggtccgcg caacgtatcc actgctttga tgtattcgtt cgcgcagaga gatgatacga
     3721 gtgtctttga tgagccgttg tatgcgcatt atctttccaa aacgccggcg cgtgcctatc
     3781 atcccggcgc ggaagaagtg atcgccacca tggaaaatga tggggagaaa gttgtggatg
     3841 atttgatatt aggggatcat gatacgcctg tcgttttttt caaacatatg acgcatcatt
     3901 tatacgaact ggatttagac ttccttgacc atacagttaa tatcctgttg actcgcgacc
     3961 cggttgaaat gctccccagc tttgccgcgt gggtcgaagc gccttcgctt cgggacgttg
     4021 gttacgcgct ccatttggat ttgctgcatc acctgcaatc gcgcggacaa aacccaccgg
     4081 tgctggactc caagcaaatt ttattaaacc cgcgaaaagt gctcaatgaa ctgtgtgagc
     4141 ggattgggat cccatttcac gaaaccatgt taagctggca ggctggtcca cgacctgaag
     4201 acggttcgtg ggcgaaatac tggtacaagt ctgtacatga atcaactggc tttggagaat
     4261 atgttaaaaa atccgcgccg tttccaaagc atctcaagcc tttgttgggg gaatgtatcc
     4321 catattacga aaagctacat gaggtggcaa ttcaggcgta atgtttagtg gggggtgaaa
     4381 agaaaaatat tccattcgct ttgatagatt caaattcctt gccgttccgt agttaatagt
     4441 agatgctgtc tggtcatctg actgcagcag atgttagcat cacctccgtc actttctgtg
     4501 gacggtcatc ccctatttag gttttaaaga gaagaaagga atatctcatg tcgcgtttaa
     4561 ctcgtttatg gaaaaagtgg ataacgccca cccaaattac gattttcagt ttgctcagta
     4621 tgctcagcct catatttcca tccgtgtcat ttgccatgca atcacagact gcctccgggg
     4681 aaacaatcta cgcgattgtg gagcgaaata gaatcatcca attttcaagt ggtaactcat
     4741 gcacggtgac ttccagtgta agaattaaag gattacagcc tggcgaacga ttggaaggga
     4801 tcgattttcg tccgctgacc gggcaattgt atggactcgg tagcaagagc cgtttgtaca
     4861 ccattgatct tgcgtctggg gttgcgactc aagttggtgc cccatttagc accccactta
     4921 acggaactct ttttggcttt gactttaacc ccacagtgga tcgcattcgc gtggtaagcg
     4981 acactggcca aaacttgcgc ctgcatcccg acacgggtgc ggtcgcggca gtggatttct
     5041 ccctggcgta tgcttctaca gatgtcaacg cgggcgcatc tctttttgtt aatggtgctg
     5101 catacaccaa cccggataat gatccaacca caggcactac tctatatgat attgacacca
     5161 gtctggatat cctggcatcc caagttccac ccaatgatgg tgttctcaac acgattggta
     5221 cgttgggcct cagtgtcaac gaacctgctg gcttcgatat atcactttct ggaatggctt
     5281 atgcagccct tcagaccagc gatacgagcg gtaaatgcgc acccacaaaa ttgtttacaa
     5341 ttgatttaac aactggcact gccacactga tcggcggcat tggagttagt gaacacattc
     5401 tcggaattgc tgttgctcca taagaaaaag actctttgat cgatggcctc acgttttgac
     5461 acgaacgtga ggctttttct tatgaattat ctaacggggc caatggagtc cctgggcttt
     5521 cgcctgggtc cattcaaaat aaccacagtt tcgaaggtgt ctatacgcct caaaaaccag
     5581 tatgcaggta ttctcgcttc aattccgcag aagggtcaca tcccgccggg aggattgtat
     5641 acttccggca tggtttcctc atcgagcaag ccagatttaa ttgcaccaac acgcatccgc
     5701 ctttgggtga gcgcgtttcg atatctggct ggcaaggcat tgacgatcct tgtcaccatt
     5761 tttatggggg tatttatcac gatgctgatc gtgaactatc ctccgggaag gggcgccgaa
     5821 ccaaccaaat caccattcca ggtgaggctg gaaagtgaaa tcgaccggtt tgtacagagc
     5881 atgttgtatt ccggaactat tcctttccag ggtcccgcag attttgaagc acaatatgag
     5941 tctctctcga atcaactgcg tgcggaagca ggcttggacc tccctttctg gccgcgtaat
     6001 ttgctctgga cgttcaacgc actgaccttc aactggggcg aattagattc gaccttcatc
     6061 cagtcgttgg gtacgggcgg agggtttgaa acagacgtgt acggcaatgc tgttttacac
     6121 ttcttaccaa ataccctatt gctggtgggc atctcttatc tgctggtgtt cctgctggga
     6181 atgccgttgg cattgtacct ggcaagaaat tacgggagcc gcctggaccg tcttgtcacc
     6241 attctctcgc cgatctcatc cgttccgagc tgggtatttg ccgttttgtt gattgccctt
     6301 tttgctgttc aactacgctg gctgcccata ggcggaatgt ctgatatttt ttatacacca
     6361 aaaacaccga tcgaaaatgc gctcactgtc ttaaaacata tggtgttgcc agtcactgcg
     6421 ctggtcctga gtcttctgtt ccagttggtc tatgcctggc gcaccttctt tattatttat
     6481 tcggaggaag attacgtcga gctggcgcgc gccaaagggc tggacaataa aaccctcgaa
     6541 aggcaataca ttctgcgacc tgctctgccc tacatcatta ccagtttcac gaccagcctg
     6601 atcggctttt ggcaattgac tgtagcactc gagagcgtat ttcgatggcc tggcattggc
     6661 ttgttatata ttgaaacatt gccaaattat tggggagaac gggtcgccat tggtgatctg
     6721 atgattgtga ttcagatcgt tgtcgtcttc gcttatttgc ttggtatgct ggttctcatt
     6781 ctggatatcg tgtacgtgct catcgatccg cgccttcacc ttacgccaaa aaacacttca
     6841 tcacttaata atgctcgcgt aaaaactaaa gctaccaacc caaaacgtct attctcaacc
     6901 gcacggatta aagatagaaa ttcggatcgc gcgtctcggg taaaagttcc aggagagaga
     6961 tgggtgttct ctccgtccag gacgatacgg gccgcgttcg attccatccg cgagatgcag
     7021 tcgcgcagca ggttattctt ccaggaattg cgcagatatc cgtctgccat ctttggattg
     7081 accatcattc ttattctgct ggcaggttcg ctttatgcgg tggttgctct gccctatgaa
     7141 gaatttggca ggagctataa cgaagaccgc gtgagagggt tcaacctgcg gccgcgcgtg
     7201 gcagctccga attggttcaa cctttttagc ccaaccccgc ggctgtccac gctgattctg
     7261 aaggaggaca acgcaggcgc gagcgtgtcc acaagcgcgc tcgataatgg ctggactcaa
     7321 aaaacagtca ctttcacctt tgaatatcca tatggcgaga tgccaagtga tgtgttcctg
     7381 tatctcgatc ccacctatgt tgaaaagttt ccctttgtat ccatggtatg gaaaacaccc
     7441 gatggtcgcg cgctgaatct taaacccgtt ggcgtcggca atgcgatcaa ttatgatttt
     7501 aaggatgggg ttcaggttcc taaattctta aatcaaaatc cggcgtggaa ggattggttt
     7561 gcagcagaag gtcaa
//
LOCUS       DASTJP010000404         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_425721, whole genome shotgun sequence.
ACCESSION   DASTJP010000404 DASTJP010000000
VERSION     DASTJP010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425721"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..774
                     /locus_tag="VFQ23_11760"
     CDS             247..774
                     /locus_tag="VFQ23_11760"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HET9907316.1"
                     /translation="MSTNRFFIPIIVIAVVVLLVMRGIGLAVSRGSPVSGDFITTSTI
                     INNVTQDKFNKIFDLSSTSTYTGTLEGTSTLHGTLTVRRDGSASFRGVENFTGLVNGT
                     PGTLTFKFEGSNDLYQEIQLDNLIIGSTGELDGLQGELSKTGIIKDNGPVGTYTGQFN
                     TTSNPNVEVEESDEY"
     gene            819..1373
                     /locus_tag="VFQ23_11765"
     CDS             819..1373
                     /locus_tag="VFQ23_11765"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907317.1"
                     /translation="MFNKAPFHMLVVITVLVITACAPQVPATSSTQPPTEPVTLSLAV
                     ADQVDRASDPYVREFIEQTKTLSNGSITIQPIWDAGAYTTPSFEEGIIKALKQGRYDL
                     GLVAARAFDTQGITSFQALQAPFLITDDALAKAVAASDISAGMLDRLSSAGMVGLTLW
                     HQNTAFREDKAVWEAKPWQKIADQ"
     gene            complement(1438..>2831)
                     /locus_tag="VFQ23_11770"
     CDS             complement(1438..>2831)
                     /locus_tag="VFQ23_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907318.1"
                     /translation="NAEAFINFHLARPGGFFLMVNLFLLLANLICVFVFYKIAVTTLE
                     NHRLLAGTAITLLYFILHPYSFPSLTFWSHNSFNFPFGTLWLLWLYSNLRKDIEIAPR
                     LLLLMGLAAGVLAAAQMYFIVWLVSGTITIFIFSWRLGKTFKESLFSSVYMVSGGIVG
                     LLLMLIPVYKELPRFAGWLTRILTHQGLYGTGERGIYTLNLIPAAINYWLVTIPLMMI
                     VLLLTLATLGAFAIWIRKASIKVSPGTYAMVIGLLFHIAFVLLVMSKAVLRLRFSLSL
                     AAILPVLILLVLKISEITPWRVSKLKPVLYTMTIFGVVSVLVQQINLQSTRAAVERDA
                     PLAKEQAVSRLSQKTNVSEEDIVVVYAFSVPLKCPGLLLASSWTGSFGSEIQSICPNQ
                     YAILDVNEGLDAEFNISGTIPHITEIDWDLVVWPGNGSNLPEYLYSIGAINIPNSWHV
                     RRDKWFFIHPIDE"
BASE COUNT          833 a          662 c          678 g          658 t
ORIGIN      
        1 actccctggc tacagatctt tcgtacattg caggcaaata cgaggataga tatgaccgta
       61 tgaacgacct aggtacagag ctcgcgtaca ttatgggcaa acatgaagac aggtatgact
      121 tcatgaatga cttcccaggc actaaccgct gatttgaaac acagtagcga ccgggtattg
      181 gtcagtgcct tgtgccagaa aatgctagcg cagggcatga aaatttgtag actacaggag
      241 cacgaagtgt ccaccaaccg ttttttcatt ccgattatcg taattgcagt agtggttctg
      301 ctcgttatga gaggcattgg attagccgta tctcgaggtt caccagtgag cggagacttc
      361 atcacaacat ccaccataat caataatgtc acacaagaca aattcaacaa gatttttgat
      421 ctcagtagta cgagcaccta taccggtacg ctggagggta catcgacact gcatggcaca
      481 ttgactgtcc gccgcgatgg tagcgcaagc ttcagaggtg tggagaactt taccggattg
      541 gtgaatggaa cacctgggac actcaccttc aaatttgagg gaagcaatga tctataccag
      601 gagattcagc ttgacaatct catcatcggc agcactggtg agcttgacgg cctgcagggt
      661 gagctttcaa agacaggcat cattaaagac aatggtcctg taggaactta cacagggcaa
      721 ttcaatacga cttcaaatcc aaatgttgag gtcgaggaat cggatgaata ctaaatctgt
      781 attcattagc aaatctacct tgtaaaaagg agaccaaaat gttcaacaag gctccattcc
      841 acatgttagt cgtgatcaca gtacttgtca tcacagcgtg tgcaccgcag gtcccagcaa
      901 cgtcttccac acagcctcca acggaacctg tgacactgag cctggccgtt gccgaccagg
      961 tagatcgcgc gagcgatcct tacgtgcgcg agttcatcga acaaaccaag acgctctcga
     1021 atgggagtat tacgatccaa cccatctggg atgctggcgc atataccacg ccgtcatttg
     1081 aggaaggtat catcaaagca ctgaagcagg gtcgatacga tcttggtctg gtagctgcgc
     1141 gtgcgtttga tacgcaaggc atcacaagtt ttcaagcctt gcaggcaccg ttcctgatta
     1201 cggatgacgc cttagcaaaa gccgtggcag ccagcgacat ttccgcagga atgttggata
     1261 ggctgtcatc ggctggcatg gtggggttga cactttggca ccaaaacacc gcatttcgag
     1321 aggataaagc ggtttgggag gcaaagccct ggcagaagat tgccgatcaa taatctgctt
     1381 gctccatggg aaaagataac agcctcgcgg atgaaaagtc cgcgaggctt tcatgcccta
     1441 ctcatcaatt ggatggataa aaaaccactt atccctgcgc acgtgccagg aattcgggat
     1501 attgatcgca ccgatcgaat ataaatattc aggcagatta ctgccgttcc cgggccagac
     1561 gaccaggtcc caatcgatct ctgtgatatg tggaatcgtg ccggaaatat tgaactctgc
     1621 gtccagccct tcgttgacat cgagtatggc atattggttt gggcagatgc tctggatttc
     1681 acttccaaac gatcctgtcc aactgctggc gagcaacaag cctgggcact tcaatgggac
     1741 tgaaaaagca tagactacta cgatatcttc ttcacttaca tttgtctttt gagataaccg
     1801 ggatactgcc tgctccttgg caagcggcgc gtcccgctcg acggcggctc gcgtgctttg
     1861 aaggttgatt tgttgtacaa gtaccgatac aactccaaag attgtcattg tataaagaac
     1921 gggtttcaat ttactcacgc gccagggagt tatctcagag atttttaata ccaacaaaat
     1981 caagacaggt agtatcgcag ccagcgacaa ggaaaatctc aatcgcaaaa ccgccttgct
     2041 catcactaat aaaacaaacg caatgtgaaa tagcaaacca atgaccatag cgtaggtacc
     2101 aggggaaact ttgatggagg cctttcgaat ccaaattgca aacgctccca gagtggccaa
     2161 agtcaataaa agaacaatca tcatcaaagg aatcgtgacc aaccaataat tgatcgcagc
     2221 cggtatcagg ttgagggtat aaatgccgcg ttcgcctgtg ccgtagagtc cctggtgggt
     2281 gaggatgcgc gttagccagc cagcaaaacg tggtaattct ttgtacacag gaatgagcat
     2341 gagcaaaaga ccgactattc cgcctgagac catatacacg ctggagaaga gagactcttt
     2401 gaaagtcttg ccaagtctcc atgaaaatat gaaaatagtt attgttccgc tcaccagcca
     2461 aacgatgaaa tacatttgcg cggcggcaag aacacctgcg gcaagtccca tcagcagtaa
     2521 taagcgcggc gcgatttcta tatcttttct taaattacta tacaaccaga gcagccataa
     2581 ggtaccaaat ggaaagttaa aggaattatg cgaccagaac gttaacgatg ggaagctata
     2641 cggatgaaga atgaaataca gtaaagtaat agccgttcct gccagcagac gatggttttc
     2701 gagtgtcgtc acggcaattt tgtaaaacac aaatacacaa atcaaatttg ccaggagtag
     2761 aaataaattc accatcagaa agaagccgcc tggtctggcg agatggaaat tgatgaacgc
     2821 ttcagcgttg t
//
LOCUS       DASTJP010000405         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_824372, whole genome shotgun sequence.
ACCESSION   DASTJP010000405 DASTJP010000000
VERSION     DASTJP010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824372"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFQ23_11775"
     CDS             <1..322
                     /locus_tag="VFQ23_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907319.1"
                     /translation="FAAVVLLMREANTLVDVSSFLVQGLSGANFPVKSLPYWLIPISL
                     MLPLTYGFDAVRGFLLKTNTLLPLPMEVALLIVFMFVMLWFGSWVFYKIERRVRTLGT
                     LGQH"
     gene            complement(423..911)
                     /locus_tag="VFQ23_11780"
     CDS             complement(423..911)
                     /locus_tag="VFQ23_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9907320.1"
                     /translation="MSKDKVEAGVVVSMEYTLHVDGELLDSSDGQGPLQFLVGYGNII
                     SGLEEEMMGMKIGDSKDVVVQPKDGYGEFDDEAFMEVPRNDFPKEMPIEVDAELTVRD
                     DAGNARYARVDRIEGDTVTLNFNHPLAGDELHFHVKVLELREPTAEELDHGHVHDAGH
                     QH"
     gene            1184..>3026
                     /locus_tag="VFQ23_11785"
     CDS             1184..>3026
                     /locus_tag="VFQ23_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9907321.1"
                     /translation="MSKVVRYSLSAVVILTLVLGSMAMTPAARSTATVHLKSGSFIPA
                     QGVSAQGSQKYFIVQFSGPVQEAWKESLTAEGAEVLDYIPDYAFKVLMNPAIANRVGQ
                     NAFVSAVVAYRPEFKLGTDLKRDGELNVYRVRIERGSDFGQVRALVAQTGAQILGFEG
                     DLMAVAAPGSFVDAIAAVDDVASISNFALNSIQLAEPTKEKANESAQDIIGANAAHSR
                     GYDGASQIAAVSDTGIGGGTAATAHTDIPSGRVVAIFNWPGVTDTCFQSITNDGAIDV
                     DSGHGTHTAGSVLSDGGAGGIGEGIAPAARLVFQATENWAVISNFCQMFGGWPANGYF
                     LTGLPADLKTMYQQAYNSGARIHSNSWGSALAGDYTLDSANTDQFVWTNRDMVITFSA
                     GNEGIDANNNGVVDNDSIGSPATAKNVITVGASENVRADNWACDTSLTYTSHDAYQPG
                     QTCNSMGGQNLLGTWGQRYGTDFDVAPLANDLTAGNSGQMASWSSRGNTDDNRIKPDV
                     VAPGTWILSAYSDLYQEGYEGDPVNPQNGIHQWDGWGMPYNNDYKYMGGTSMSNPIAA
                     GAATIVRDYYNEAKSHPNSSAALIKATLINSAVDMADENNDGANDNDF"
BASE COUNT          724 a          813 c          759 g          730 t
ORIGIN      
        1 gttcgcggcg gttgtcctcc tgatgcgcga ggcaaataca ctcgtcgatg taagcagttt
       61 cctcgtacag ggactctcgg gtgcaaactt cccggtcaaa tccctaccat actggctgat
      121 ccccatttca ctgatgctcc cgctgaccta tggctttgat gcagttcgcg gtttcttatt
      181 gaagacaaac acccttctgc ctctccccat ggaagtagcc ttgctcatcg tctttatgtt
      241 tgtaatgttg tggttcgggt cgtgggtttt ctataaaatc gaaagacgcg tgcgcaccct
      301 gggaacactg ggtcaacact aatataaaaa aagacatctc ctcactacaa gttcgatgtc
      361 tttaaatggc ttccccttct ccctgcgtcg tagggagaag gggaaggggt gagcaacctg
      421 acttaatgtt gatgccccgc atcatgaaca tgtccgtggt ccagttcttc ggctgtaggc
      481 tcacgcagct ccaacacttt cacatgaaaa tgcagttcat ccccggctaa tggatggttg
      541 aaattcaagg tgacagtatc accctcgatt cgatcgacgc gcgcatagcg ggcattgccc
      601 gcatcatcgc ggacggttaa ttcagcatcc acttcaatag gcatttcttt ggggaaatca
      661 ttgcgcggca cttccatgaa ggcctcatca tcaaattctc cataaccatc tttcggctgg
      721 acaaccacgt ctttgctgtc gccgattttc attcccatca tctcttcttc cagtccagag
      781 atgatgttgc catatccaac taaaaactgt aatggtccct gcccatctga agagtccagc
      841 aattcgccat ctacatgaag tgtgtattcc atggaaacaa ccacgccagc ttctacttta
      901 tctttgctca tcgtacttcc ttcttccttt caaacttgag tttgccttga ggcacgtcca
      961 ttgtaatccg tttttgtaac ctactacaat ttatcgtctg atacaaaatg gtaaacttcg
     1021 cgtctggttt aaaagaagaa tcgtgattga cagaaaataa atccagagta aaatcgtcaa
     1081 tctaagattt tctgtcaacc aataaaattc acataccaac tttgtgttaa acgttgatgc
     1141 gtagatcaac gttttctaat ttcaaccaca gaggagagtg cgtatgagca aagtagtcag
     1201 gtatagtttg tctgctgttg tgattctgac ccttgtgttg ggatcgatgg caatgacacc
     1261 cgccgcgcgt tcaactgcca ccgtgcattt gaagagcggc tcttttattc ccgcgcaggg
     1321 agtcagcgcg caggggagtc aaaagtactt catcgttcag ttctcaggcc cagtccaaga
     1381 agcatggaaa gaatcgttga ctgccgaggg cgcagaagtg cttgattaca tcccagacta
     1441 tgcattcaaa gtgctaatga acccggcaat tgcgaatcga gtgggtcaga acgcctttgt
     1501 cagcgccgtg gtcgcatatc ggcctgagtt caagctcggt actgatttga agcgcgacgg
     1561 cgaattaaat gtctaccgtg tgcgtatcga acgcggatcc gatttcgggc aagtccgcgc
     1621 gctggtcgcg cagacaggtg ctcagatcct cggcttcgaa ggggatttga tggccgtagc
     1681 agctcccggc tcctttgtgg atgccattgc tgctgtagat gatgtcgctt cgatcagcaa
     1741 tttcgcattg aacagcatac aacttgcgga gcccaccaaa gagaaggcta acgagtctgc
     1801 acaggatatc atcggcgcga atgccgcaca ctctcgcggt tatgacggcg cgtctcagat
     1861 cgcggcagtc tctgatacgg gtatcggtgg cggaacggca gcgacagctc atacggatat
     1921 tccatcgggc cgagtggtcg ccatcttcaa ctggccgggc gtaacagata cttgtttcca
     1981 gtctattacc aatgacggcg caatcgatgt agattccggc catggcacgc acactgccgg
     2041 ttctgttctt agcgatggcg gcgccggtgg aattggtgaa gggatcgcac ccgcggcacg
     2101 tctggtcttc caggctacgg aaaactgggc cgttatttcg aacttctgcc agatgtttgg
     2161 cggatggccc gcaaacggtt atttcctcac tggcttgcct gccgatttga agacaatgta
     2221 ccagcaggcg tataactcag gcgcacgtat ccattccaac tcatggggct cggccctggc
     2281 tggcgactat actctggata gtgccaacac cgatcaattt gtgtggacca accgcgacat
     2341 ggtcatcacc ttctcagcag gcaatgaagg catcgatgcc aacaacaatg gcgtggtaga
     2401 caacgactcg attggctcgc ctgcgactgc aaagaatgtg atcacagtgg gcgccagcga
     2461 aaacgttcgc gctgataatt gggcttgcga tacaagcctt acttacacct cgcacgacgc
     2521 ctaccagccc gggcagacct gcaacagcat gggcgggcaa aacctgctgg gcacgtgggg
     2581 tcagcgctac ggaactgatt ttgatgttgc cccgctcgcc aacgatctga ctgctggcaa
     2641 cagcggacaa atggcatcct ggtcgagccg cggcaatacc gatgacaatc gcatcaaacc
     2701 cgatgtcgtc gcaccgggaa catggattct ctccgcctac tcggacctgt atcaggaagg
     2761 ttatgaaggc gacccggtca acccgcagaa cggcatccat cagtgggatg gctggggtat
     2821 gccctacaac aacgattaca agtacatggg cggcacatcc atgtcgaacc cgatcgctgc
     2881 gggcgcggcg accatcgtca gggattacta caacgaagcc aagagccatc ccaactccag
     2941 cgcggcgctg atcaaagcca ctttgatcaa ttcagcggta gatatggcag acgagaacaa
     3001 cgatggcgcc aacgacaacg acttcc
//
LOCUS       DASTJP010000406         6554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1310685, whole genome shotgun sequence.
ACCESSION   DASTJP010000406 DASTJP010000000
VERSION     DASTJP010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310685"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFQ23_11790"
     CDS             complement(<1..264)
                     /locus_tag="VFQ23_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9907322.1"
                     /translation="MEAPKGYLLVVEDIPDILKLLEATLTFKGYRVVTAKNGEEALAA
                     IERERPLLVVTDILMPKMDGFSLVHRLRINPDTRNIPVVFLSAT"
     gene            659..1480
                     /locus_tag="VFQ23_11795"
     CDS             659..1480
                     /locus_tag="VFQ23_11795"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9907323.1"
                     /translation="MKKVTLAIVLALTMVFSTIGFASAITNGQPDGDNHPYVGLAVFD
                     VLDENGETVPSHRCSASLLTPTVVLTAGHCTDGTSAARIWFDEIVQGNPEYPFSGATS
                     YDGVAYTFPGFCVGCGNGLPGFAAGDVGIIVLSEPVPTSVVSEYAELPDENLIDTLQN
                     KTGIDFVGYGVQEQIVGGGPPVWAGLRNRLFAPSELVSGKFKHGDQFMKLALNPGGGS
                     GGTCFGDSGGPDLLAGTNIVLAVNSYVTNSNCTGVGYGARVDTPDVLAWINTFLQ"
     gene            1636..2859
                     /locus_tag="VFQ23_11800"
     CDS             1636..2859
                     /locus_tag="VFQ23_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907324.1"
                     /translation="MRTIFALILKFVAFILAFFAIFATIFALLFLSFNRILLAPQTYK
                     QAFIENKVYEKLPEVTATEFALIKGHLVEPCMEAIIADSCIDSVVNSPTVETGKLGME
                     GSNLIQVLHQEQWNGLVFYLLTPDAVQRSLDAGVNEVIAYFKGETASAGIPLSDVKEE
                     LASIRGNELATIFLNQQPSCTLEQQTRIMSGALDEIGSTAVFCSATGGTSDLLLLDLQ
                     RRSNSIASELPEQVVLLKPPSPSNPAGLQRIIGKDLQATLQAIQINAQYIPVLPGVLL
                     LLVTIINVRSLRGLLRWWSLPVFIGSLVALIVGVILFFLFQQMWLNYVLSDSSPILTS
                     GFGDILYSTTHSLARNFSQHLMTQAGIATLITLSVLLISNRLPAPPDPSLPPLAPPGT
                     PGGPVLNPNQKKKKW"
     gene            complement(2941..4740)
                     /locus_tag="VFQ23_11805"
     CDS             complement(2941..4740)
                     /locus_tag="VFQ23_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907325.1"
                     /translation="MTEIPFTEKVPSLPAWRVIWEMIRFRPWLWFVDLLSVALIRFCW
                     QIAPALIIKAFFDMLTGAAPLTFGIWAIVAFALTSWIGRVLASYGFYYADVPIFADMP
                     TLLRKNLLKHILRRPGAATLPDSAGEAVSRFKKDVEEIPLFVILINDVMVGIGIIAYS
                     IFLMSSISSSVTVMALIPLLIVGLIANLATSRIEHYRTASRQATGQVTGFIGEFFGAV
                     QAVKVAAAETNVIGHFHRLNDERRKLTIREKLFDDVLGSIYRNTSTFGTGVILILVGQ
                     SMRAGTFTLGDFSLFVYLLQSMGDLTTFAGMLAARYKQLNVSVQRMYRLMENAPLDAL
                     VEHSKVDLEGDLPEVVYPMKSTSDRLDVLVATKLKFHYPNSTNGIDDVDLTLKRGTVT
                     VITGRIGSGKTTLIRSLLGLLPIESGEIRWNGNLISSPGDFFIPPRCSYTAQVPRLFS
                     NTLRNNILLGLNRSDDDVYRATKLAVMDRDLEDLDDDLETMVGARGVKLSGGQAQRTA
                     AARMFIREPELLVFDDLSSALDVETERQLWDRIFTQQDKTCLVVSHRRPLLRRADHIL
                     VLKDGRVESEGKLEMLLETSAEMQELWNLEKNL"
     gene            complement(4751..6514)
                     /locus_tag="VFQ23_11810"
     CDS             complement(4751..6514)
                     /locus_tag="VFQ23_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907326.1"
                     /translation="MKLPFKAYWDLLSQHIRPQKGRFVLLSILLLGSIGLRIYAPQVM
                     RMFIDSALAGEALQTLTLTAVAFIGIALVQQIAAVSVTYLGENVAWTATNALRAELAW
                     HALNLDMRFHNDHTPGELIERIDGDVTELATFFSQFALNLVSNGLLLVGILTVLFIED
                     WRAGLAFSVFSLATILILGRLKDIAVPHQKARREAEAQLYGFVEEQLAGTEDIRSSGA
                     VDYSIRELFRHQGIILGHNRKAHFKRWIIENAMGLALTTGSLLALTSGYWLFTAGLVT
                     IGTVYLFVHYINLLEEPFWAMTHEIESFQTIGACVERLTEFRNFKAEVLDGSLEPVLA
                     AAGEANKPLAIEFDKVTFSYNGRDSVLNQLSFDLTPGSVLGLLGRTGSGKTTLVRLVF
                     RLYDPQAGCIKINGLDLRDMKIESLRRSVAMVTQDVQLFRASIRDNLTFFDRSISDDN
                     ILATLEELELGDWYRSLPNGLDTELDMGARSLSAGEAQLLAFTRVFLRNPGLVILDEA
                     SSRLDPATEQRLERAIDKLLKNRTAIIIAHRLGTVQRADEIMILESGDVIEYGERKQL
                     AAESQSRFYGLLQTGLEEVLA"
BASE COUNT         1680 a         1659 c         1569 g         1646 t
ORIGIN      
        1 ggttgcagag agaaatacca ctggtatatt tcgcgtgtct ggattgatgc gcagacgatg
       61 caccagacta aacccgtcca tcttcggcat caggatatcg gtgacaacga gtaacggccg
      121 ctctcgctca atagcagcca atgcctcttc tccattcttt gctgtgacta cgcgatatcc
      181 cttgaatgtg agtgtggctt ccagcaactt taggatatcg ggaatgtctt ctacaaccag
      241 taaataaccc tttggagctt ccataattct attctaatcc gtatttcctc tttacgaaat
      301 gatactatgg ttctgctatt ttacagaaac cagacccgta cgtactccga gctcttctaa
      361 gatatctctc agataacact tattcattta gggttggcac atgagcaaag tgcttgcctt
      421 ttttatatcc gtggcatata gtgcagatag attccgggag tgatgaagaa tcagaaagtg
      481 tcaatggatt tgcatggctt gagaccgtca gaatactgga gcttttctcc atcctggaat
      541 cccatccgaa aggaggcgat gcactaaccc ttaatcctac tgactgatcc tttcgcccac
      601 cgctcattcg tgagaaggaa tgtaaaggat caaacacaaa cctaaggaga taaaacgaat
      661 gaagaaagta actcttgcga ttgtgcttgc cctgactatg gtctttagca caatcggctt
      721 tgcaagcgca atcacaaacg gtcaacccga tggtgacaac catccgtatg tagggctggc
      781 ggtgtttgat gtgttagacg aaaatggtga aacggtgccc tcgcaccgtt gttctgcctc
      841 cttgcttacc ccgactgttg tcctcacagc gggacactgc acggacggga catctgccgc
      901 gcgcatctgg tttgatgaga tcgtccaggg gaatcctgaa tatcccttta gcggagccac
      961 atcctatgat ggtgttgctt ataccttccc cggtttttgt gttggctgtg gaaatggttt
     1021 gcccggtttt gccgctggcg acgtgggcat catcgttttg agtgagcccg ttccaaccag
     1081 cgttgtgagt gaatatgcag aactcccaga tgaaaacctg attgataccc tgcaaaacaa
     1141 aaccggtatt gactttgttg ggtatggcgt ccaggaacag atcgttggcg gtggacctcc
     1201 agtttgggcc ggacttcgaa accgactctt cgctcccagt gaattggtct ctggaaaatt
     1261 caagcacggt gatcagttta tgaaactggc attgaatcct ggtggtggct ccggtggaac
     1321 ctgcttcggt gactcgggtg gtccggatct cctggctggc acaaatatcg tccttgcagt
     1381 caattcgtac gtgactaact ctaactgcac aggcgttggc tatggtgccc gtgtagatac
     1441 acctgacgtg ctggcttgga ttaacacctt cctccagtaa gttagcttct cctgaaaaac
     1501 gatggagccg ccatctggcg gctccatttt tgagtttttt aggcaaattc tgcataaatc
     1561 tcaatctaca aaaacctaac aaccctcgca ttttgactcc ttttgctata tgatttaacc
     1621 ggaaggagaa tgataatgcg gaccatcttt gccctgattc ttaaatttgt ggcatttatt
     1681 ctggcttttt tcgctatttt tgccacaatt ttcgctctgc ttttcttgag tttcaatcgt
     1741 attttgcttg caccccaaac ttataaacaa gccttcatag agaataaagt ttacgaaaaa
     1801 ctccctgaag tgacggcaac ggaatttgcg ttgatcaaag gtcatttagt agaaccatgt
     1861 atggaggcca taattgctga ctcttgtatc gactctgttg tgaattcacc gaccgtggaa
     1921 acgggtaaac ttggaatgga gggaagcaac ctcatccagg tgcttcatca ggaacaatgg
     1981 aatggtctcg tcttctattt actcacacca gatgctgttc agaggagcct ggatgcaggc
     2041 gtcaacgaag tcattgcata tttcaaaggc gaaacagctt cggccggaat acctctgagt
     2101 gatgtgaaag aagaactggc gagcattagg ggtaatgagt tagcaacaat atttttgaat
     2161 cagcagccta gttgtacctt ggaacaacaa acacggatca tgtctggtgc gctcgatgaa
     2221 atcggcagca cggcggtttt ctgctcggcg acaggtggaa catcagacct cctcctcctg
     2281 gatttgcaaa gacgatcaaa tagcattgcg tcggaattac cagagcaggt agttcttctc
     2341 aagccaccct ctccatcaaa cccagccggg cttcaaagaa tcattggaaa agatcttcag
     2401 gccacattac aagcaatcca aattaatgca cagtacattc ctgtgctacc aggggttttg
     2461 cttttgttgg tcacaattat taacgttcgt tcattgcgcg gcttgttacg ctggtggagt
     2521 cttcccgttt tcatcggcag cctggtagca ctaatcgtgg gagtcatatt attttttttg
     2581 tttcaacaaa tgtggttgaa ttatgtgttg agcgattcgt cacctatact tacatccggt
     2641 tttggagaca ttctttacag taccacacat tccctggcaa ggaatttttc tcaacacctg
     2701 atgacgcaag cggggattgc gactctgata acgcttagcg ttctgctgat ttcaaatcgt
     2761 ttgcctgctc cgccggatcc gtctcttcca cctcttgctc ccccggggac cccaggcgga
     2821 ccagttttga atccaaacca gaagaagaaa aagtggtgat cgattactca ccaaataaga
     2881 cgaattaaaa tgctgaccca cattacatta actgtcttat ttggtgaggg cacatgagat
     2941 ttacaaattt ttctccaaat tccaaagttc ctgcatttcc gcgctcgttt ctaataacat
     3001 ctctaattta ccttccgatt cgacacgtcc atctttcagt accaggatgt gatccgcgcg
     3061 gcgcaacagc gggcgccgat gggagacaac gagacaggtc ttgtcttgct gtgtaaagat
     3121 tctgtcccag agttggcgct ccgtttctac atccagtgcg ctggagagat catcaaacac
     3181 caacagttcg ggttcacgga tgaacatgcg ggccgccgct gtacgttggg cctgaccgcc
     3241 agataactta acgccgcgcg ccccaaccat cgtttcaaga tcatcatcca agtcctcaag
     3301 gtcacgatcc atcaccgcga gtttcgtcgc ccgataaacg tcatcatcgc tgcgatttaa
     3361 tccgagcagg atgttgtttc gcagcgtatt actaaacagg cgcggaactt gggccgtata
     3421 tgaacaacgc ggcggtatga aaaaatctcc gggtgacgag atgaggttcc cgttccagcg
     3481 aatctctccg gattcaatcg gaagcaggcc caacaaggag cgtatgagcg ttgtcttacc
     3541 ggaaccaatt cgaccggtga tcacggtcac ggtgccgcgc ttcagggtca gatctacgtc
     3601 gtcgatgccg ttggtcgaat tggggtaatg gaatttcaat ttggtggcga ccagcacatc
     3661 gagtcggtct gaagtgcttt tcatcggata gaccacctca ggcagatcgc cctctaaatc
     3721 cactttgctg tgctccacta atgcatccaa cggtgcgttt tccattaatc gatacatgcg
     3781 ttggacagat acattcaatt gcttgtaacg cgcggcgagc attcctgcaa aggtagtaag
     3841 gtcgcccatg ctctgcagga gatagacaaa caaagagaaa tcgcccagcg tgaacgttcc
     3901 cgcgcgcatg gactgaccca caaggatcaa aatcacaccc gtcccaaacg tagacgtatt
     3961 ccgatagatc gatccgagta catcatcgaa taacttttca cggattgtga gcttgcggcg
     4021 ttcatcattg agtctatgaa agtgaccaat gacattcgtt tctgcagccg cgactttgac
     4081 ggcctgtact gccccaaaga actcaccgat aaaaccggtt acctggccag ttgcctgacg
     4141 cgatgccgtc ctgtaatgct caatacgact ggtggcaaga tttgcaatga gtccgacgat
     4201 gagcagcgga attagcgcca ttacggtaac cgacgagctg atgctggaca tcaggaatat
     4261 cgagtaagca atgatgccga taccgaccat cacatcattg ataaggatca caaagagagg
     4321 aatctcctcc acatcctttt tgaaacgact gacggcctca cccgctgaat cgggcagtgt
     4381 agccgcgccg ggccttcgca gaatatgctt taggagattc tttcgcaaca gcgtaggcat
     4441 atcggcgaag atgggtacat cggcgtaata aaaaccgtaa ctagcgagta cacggccgat
     4501 ccagctggtt aacgcaaatg cgacgattgc ccagatgcca aaggtcaatg gtgcggcgcc
     4561 tgtcagcatg tcgaagaaag ctttgataat gagggccgga gcgatctgcc agcaaaagcg
     4621 gatcaacgct acggagagca agtccacgaa ccaaagccag ggccgaaagc gtatcatttc
     4681 ccagatgacg cgccaggcag gcagggacgg aactttttcg gtaaagggta tttcagtcat
     4741 gatatgcctc ttatgccaac acttcttcca acccagtttg tagcagccca taaaaacgcg
     4801 attgcgattc tgcagctagt tgcttacgtt caccatattc gatgacatct ccgctttcca
     4861 ggatcatgat ctcatcggcg cgctgtacgg ttcccaaacg gtgagcaatg atgatcgccg
     4921 tgcgattctt taacagcttg tcaatcgctc gttccaggcg ttgttccgtt gccggatcca
     4981 aacgggatga agcctcgtcc aggatcacaa gccccggatt gcgtaagaag acgcgtgtga
     5041 acgccagcag ttgcgcctcc cctgcggaca aggaccgggc tcccatatcc agttcagtat
     5101 ccaggccatt cggcaaagaa cgataccagt cacccaattc caattcctca agagtagcca
     5161 gaatattgtc atccgaaatt gatctgtcaa agaacgtcag gttatcgcga atgcttgcgc
     5221 gaaacaattg gacgtcctgc gtaaccatcg caacgctacg tcgcagcgac tcaattttca
     5281 tgtcgcgcaa gtccagtccg ttgattttta tgcaaccggc ttgcgggtca tataatcgaa
     5341 agaccaatcg aaccaaagtg gttttgccgc tcccggttcg acccagcaac cccaacacgg
     5401 aaccaggagt caggtcgaag gagagttgat ttaacaccga gtctctcccg ttatatgaaa
     5461 atgtgacctt gtcgaattcg atagcaagcg gtttatttgc ttctccggca gcagccaaga
     5521 cgggctcgag gctgccatca aggacctcgg ctttgaaatt gcggaactcc gtcagccgtt
     5581 cgacacaagc accaatggtc tgaaaacttt cgatctcgtg cgtcatcgcc cagaagggtt
     5641 cttccaggag gttaatgtaa tgcacaaaca gatacaccgt gccaatcgtg accagccctg
     5701 cggtaaataa ccaatatcca ctggtgagcg cgagcagcga tccggttgtg agcgctaatc
     5761 ccatggcgtt ctcaatgatc cagcgcttga aatgagcctt gcggttatgc cccaggataa
     5821 tgccctggtg ccggaataac tcgcgaatgg aataatccac tgctccgctg gagcggatat
     5881 cttctgtccc ggcgagttgt tcctccacaa aaccatagag ctgtgcttcc gcttcccggc
     5941 gcgctttttg atgcggaacg gcaatatctt tgagtcgtcc gagaatgaga atggtggcaa
     6001 gtgagaagac agagaaagcc aatccggctc gccaatcctc gataaagagc acagtgagaa
     6061 tgccaaccaa caataggcca ttgctgacaa gattcaaagc aaactgcgaa aagaacgtcg
     6121 ccagttcggt cacatcacca tcgattcgct cgatcaattc gcctggtgtg tgatcattgt
     6181 ggaagcgcat atcaagattc aacgcatgcc aggcgagttc ggctcgcaga gcattggtcg
     6241 ccgtccacgc cacattttcg cccaggtaag taacactgac tgccgcgatc tgctgaacaa
     6301 gcgcaatgcc gatgaaagcc actgcggtga gcgttagggt ttgtagtgct tctcctgcca
     6361 gcgccgaatc gatgaacata cgcatgactt gtggagcata aatacgcaag ccaatgctgc
     6421 caagcaataa aatgctcaag agcacaaacc gtcctttttg cgggcgaatg tgttgagaaa
     6481 ggagatccca gtaggctttg aatggtagtt tcataagtac tctctagatt tcatcataat
     6541 taacaaaaaa ccac
//
LOCUS       DASTJP010000407         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_387971, whole genome shotgun sequence.
ACCESSION   DASTJP010000407 DASTJP010000000
VERSION     DASTJP010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387971"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFQ23_11815"
     CDS             complement(<1..392)
                     /locus_tag="VFQ23_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907327.1"
                     /translation="MAKVKKNIVMSGLSGSLGPDHYARITRDGRTIISAKPDFSNRQF
                     SEGQLSHQTRMQQAAAYAKAAKDNSVYIKKAEGTAKNAYNVALGDWFKPPVIDRIQWQ
                     EGKVRVSAHDDVMVTRVTVTILGAEGQCL"
     gene            1069..1683
                     /locus_tag="VFQ23_11820"
     CDS             1069..1683
                     /locus_tag="VFQ23_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9907328.1"
                     /translation="MPRRTTPFVPDIYYHFYNRGNNRQAVFFESDNYLYFLRGLKKYL
                     LPVAHILAYCLMPTHYHILVRIRQTSEVLETSEVSKQVSLAMQKFLISYTKAINKRFQ
                     RVGALFQGQFQAKPIQSYPHLLNLCIYIHGNPVKDGLVKAPEDWIYSNYLEWLGQRDG
                     PLVDREFIQEHFSSPAEYRELVTPFVKTRYLTDDVRKYLQSLEA"
     gene            1739..3262
                     /locus_tag="VFQ23_11825"
     CDS             1739..3262
                     /locus_tag="VFQ23_11825"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HET9907329.1"
                     /translation="MPITLDGSSLTIEKLVAIARHNEKVELAPEALERIKVCRAMLEE
                     KLAKKEVMYGTNTGIGEFSETMLNDEQVKEFQKYLIYNHAAGIGEPAPIEHVRAALAG
                     RVNVHAHGNSGCRPEITLTLVEMLNKGVTPVVCQKGSVGASGDLAPMAQAALLLMGEG
                     EAFYQGERLAGAEAMQRAGIEVPGLQARDGLAAINGSNLLTAMSALHIYDMERFLKQA
                     EIAAAMTIEALLGNLKPYNTKLHELRGFSGAVTSAKNIMKVLEGSDLTTGKMKTKVQD
                     AYSMRSTPQVIGAARDAVAYARSQVEIELNGVGDNPIFVPELNLTLTGANFQGTPVSL
                     PMDMAGIAITMISVLSERRMNRLTNPALSQGLPDFLAHEPGFYSGMMLSQYTADHLIV
                     EQRILSAPASIQSIPAAADQEDFVSMGLNTALKNGQILDNAYGVLGIEFMAAAQALDF
                     REFTPGKGTNKAREVIRKYVEHLHVDRPLYNDHNAMKKLVKSGEILEEVEKVVGELG"
BASE COUNT          821 a          835 c          815 g          838 t
ORIGIN      
        1 aggcactgcc cttcggcacc taagattgtg acggtaactc tcgtcaccat cacatcgtcg
       61 tgcgcactca cacggacttt tccctcctgc cactggatgc ggtcaatgac cggcggcttg
      121 aaccagtcac caagcgcgac attataggca ttcttggctg tgccctcggc ttttttgatg
      181 taaacagaat tatcttttgc agccttcgcg tacgcggccg cttgctgcat acgtgtctgg
      241 tgactaagct gcccttcact aaactgtcgg ttgctaaaat cgggcttcgc actgataatc
      301 gtcctgccat cgcgtgtaat gcgggcataa tgatcgggac caagtgaccc actcaacccc
      361 gacatgacaa tgttcttttt taccttcgcc atccgtttgc ttttcccttt ctgtttcatc
      421 gaaaaataac cagcccggct atatcctggc tatatcgcgc tcatatcccg cccatatcgt
      481 agccggtgtg tagcttcgct tttccttgtc tgacgcttcg attgtacatg taggctactc
      541 ttacagactt tggagtcagc ttacagttac agcccacttc gttatggatt gcacctaatc
      601 aacagcggag accgatttgg tctccgcttt ctgttttccc ataacataag tgactgtcaa
      661 ctattgctcg cgctgcaatt ctagcgccag ggttcggcat cttctggccc gcacaaaatc
      721 tgtcgtaagg tttttcccgg cagaggcgaa ccgccgcctt ttctgtactc accgtattgg
      781 attacttcat ccggttcgtc aagttgtaat tgttcttcag acctaggatg atgccctcat
      841 aaaacctggc gtcggtattc aatatagcaa cagttcattc ggagtacaat attaattagc
      901 tgaccccctc cgcccttcgg acacctcccc caaatgcgac aaagttcagt cgcatttggg
      961 gaggatgggt gggggtctta acctcaatta ggagcctgcc catgcccata accctcgatg
     1021 gtttacaaga gacttcggaa gtctaaatac cagagtgcgg agagcatcat gccgcgtagg
     1081 acaactccct ttgtcccgga tatttattac catttctata atcgcggtaa taatcgtcag
     1141 gcggttttct ttgaatcaga taactatctg tacttcctga gagggttgaa gaagtatctt
     1201 cttcctgtcg cacatattct tgcttattgt ttaatgccga cccattatca tattttggtg
     1261 aggataagac agacttcgga agttttagaa acttccgaag tctcgaagca agtttccctc
     1321 gcgatgcaaa agtttctgat ttcctacact aaggcgatca acaagcgctt tcaacgagtt
     1381 ggagcgctat ttcaaggaca attccaggcc aagccgattc agagctatcc ccatctgctt
     1441 aatttgtgta tttatattca tggcaatccc gtgaaagatg gactcgtgaa agcgcccgaa
     1501 gattggatat actccaacta tctcgaatgg ctcggtcaga gagatggacc tttggtagat
     1561 cgtgagttta ttcaagagca ttttagttcg cctgctgaat atcgcgaact tgtcactccg
     1621 tttgtcaaaa ctcgttattt gactgatgat gtgagaaaat atttgcaatc attagaagcc
     1681 taaaaaacca gacttccgaa gtcttggact tcggaagtct aattactagg aggagcccat
     1741 gcccatcacc ctcgatggat catcccttac catagaaaaa ctcgttgcga ttgcacgaca
     1801 taacgaaaaa gtagagcttg cccccgaagc actcgagcgc atcaaagtct gccgcgccat
     1861 gctggaggag aagcttgcca agaaagaagt catgtatggc acgaacaccg gcatcggtga
     1921 gttttcagag acgatgctca atgatgaaca ggtaaaagag tttcagaagt atctcattta
     1981 caatcacgct gcaggcatcg gcgaacccgc gcccatcgaa catgtgcgtg cggcgctggc
     2041 agggcgggtc aacgtccatg cgcatgggaa ttccggctgc agacctgaga tcaccttaac
     2101 actggtggag atgctgaaca aaggcgtcac accagttgtt tgtcagaagg ggtcagtggg
     2161 cgcgagcggc gacctcgctc cgatggcaca agccgcgtta ctgctcatgg gcgaagggga
     2221 agctttttat cagggcgaac ggctggccgg tgcggaagca atgcagcgag cgggaatcga
     2281 agtcccgggc ttgcaggcaa gggatggtct ggcggcgatc aacggatcca atcttctgac
     2341 ggcaatgtcc gcgttgcaca tctacgacat ggaacgcttc ctcaagcagg cagagatcgc
     2401 ggcggcgatg actatcgaag ccctgctcgg aaatctcaaa ccctataaca ctaaactgca
     2461 tgagttgcgc ggttttagcg gcgcggtcac atctgcaaaa aatatcatga aggtactcga
     2521 aggctctgat ctcacaaccg gcaagatgaa gaccaaagtg caggatgcct attcgatgcg
     2581 ttcgacgccg caggtgatcg gcgcggcgcg cgatgcggtg gcgtatgcaa ggtcacaggt
     2641 tgaaattgaa ctcaatggtg ttggtgataa cccaatcttc gtgcctgaac tcaacctgac
     2701 tctcaccggc gcgaattttc aagggactcc cgtttcattg ccaatggata tggcaggtat
     2761 tgcaattact atgatttccg ttttatcaga gcgacgtatg aaccgcttga cgaacccggc
     2821 tctttcgcag ggactgcctg actttcttgc ccatgagcct ggcttctact ccgggatgat
     2881 gctcagccaa tatactgccg accatttgat cgtggagcag cggattttat ctgcgcccgc
     2941 cagcattcaa tccatccctg ccgcggcaga tcaggaagac ttcgtctcga tggggttgaa
     3001 cacggctctc aagaatggac agatattaga taacgcatat ggcgttttag gaattgaatt
     3061 catggctgcc gcccaagcac tggacttccg tgaattcacc cctggcaaag gaacgaacaa
     3121 agcgcgggaa gtcatccgca aatacgtgga gcatttgcat gtggatcgtc cgctgtacaa
     3181 cgatcataac gcgatgaaga aactggtgaa gagtggagag attttggaag aagtcgagaa
     3241 ggttgtagga gagttgggat aagaaagatt cgtctctgga ggactcctag attaatccaa
     3301 aaaaggaca
//
LOCUS       DASTJP010000408         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_960749, whole genome shotgun sequence.
ACCESSION   DASTJP010000408 DASTJP010000000
VERSION     DASTJP010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960749"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1311)
                     /locus_tag="VFQ23_11830"
     CDS             complement(28..1311)
                     /locus_tag="VFQ23_11830"
                     /inference="COORDINATES: protein motif:HMM:NF025665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HET9907330.1"
                     /translation="MTVPTPRLIAFYLPQFHPIPENDLWWGKGFTEWTNVTKAKPLFE
                     GHRQPHLPADFGFYDLRVRETRRDQIDMAKRYGVDGFCYHYYWFSGKRLLNFPLDDML
                     ADPESDMPFCLCWANENWTRRWDAADHEILIAQKYMPEDDLQFIQSLVPFFKDKRYIH
                     VDGKPYLIVYCPQHLPDARRSVDIWRNHCRSVGVGEIHVSAALTRGNDEYAKFGFDSG
                     VEFPPHNMRVQNVNAQVKFYGPFWGNVMQYAEITRSYLDRTYKDERIFKTVVPSWDNT
                     ARTKERAVVALNGVPDNYEFWLASTIDLVGQSGRTDELIFINAWNEWAEGCHLEPDRW
                     FGHGFLQATLNAKNGMRRFTTSPETSVPSEGVVTQQALLKDIGRVVQYHIGIRFGGFK
                     LAINRWPWLRAGLIPFVKAVNVFRAKARQLMQRDQ"
     gene            complement(1407..2129)
                     /locus_tag="VFQ23_11835"
     CDS             complement(1407..2129)
                     /locus_tag="VFQ23_11835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9907331.1"
                     /translation="MNCHPPKISVGIVVYNGVGHIRHALDSIIRQTYKNIELVIVDGG
                     SDDGTQAILNEYAQFITVLICEPDKGIYDAMNKVCSIATGDWLIFLGCDDVLLDSLGK
                     MASLLNRADTVYYGDVIKRSSGKIYGGKFSKFRLMRHNICHQALMYPKSVYRKYSYSL
                     EYPARADHAYNLRLVGDEVPFVYTGVVVSIYNDEGRSASGDANFNRDQIKLIRASCGS
                     MYALIESARRLRFQLVKRMRSV"
     gene            complement(2254..>2435)
                     /locus_tag="VFQ23_11840"
     CDS             complement(2254..>2435)
                     /locus_tag="VFQ23_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907332.1"
                     /translation="LKVMGYDLSDLHRELDSAKMSWQHVISDGVALLNMKNRTANFLR
                     GIWRGSFSPAGSPRL"
BASE COUNT          640 a          673 c          524 g          598 t
ORIGIN      
        1 catcacagac cgcaatcaca gatcaggtca ttgatcgcgc tgcataagct gccgggcctt
       61 cgctctaaac acgttcaccg cctttacaaa tggaatcaga ccagcgcgta gccacggcca
      121 acggttgata gctagcttaa aaccaccaaa ccggatgcct atgtgatatt gaacaacacg
      181 gccgatatcc ttcaacaacg cctgctgagt gacaacaccc tcagacggta cgcttgtctc
      241 aggggaggtt gtgaatctac gcatgccatt ctttgcgttc agtgtcgctt gcagaaaacc
      301 atgaccaaac caccggtccg gctcaagatg gcaaccctcc gcccattcgt tccaggcatt
      361 gatgaatatc aactcatcgg ttctgcccga ttgccctaca aggtcaatgg ttgaggcaag
      421 ccaaaactca taattgtcag gcactccatt gagagcaacc accgcccgtt cctttgtccg
      481 tgccgtattg tcccaggagg gaactactgt tttaaagatg cgttcatctt tatacgttct
      541 gtccagatat gaccgtgtaa tctcagcgta ttgcatgaca tttccccaaa atggaccgta
      601 aaacttgacc tgtgcgttga cattctgcac acgcatatta tggggaggga actcgacgcc
      661 actatcaaaa ccgaacttgg catactcgtc gttgccacga gtgagggcgg cactgacatg
      721 aatttccccc acccccactg accgacagtg attacgccaa atgtcgaccg atctgcgagc
      781 atcaggaaga tgctgcgggc aatacacgat aagatatggc ttcccgtcca catggatata
      841 gcgtttgtct ttaaaaaatg ggacgaggga ctgaatgaat tgaaggtcgt cttccggcat
      901 gtacttctgt gcaatcagaa tttcgtgatc agccgcatcc cagcggcgcg tccaattttc
      961 atttgcccag cagaggcaaa agggcatatc gctttccgga tcggccagca tatcgtccag
     1021 cggaaaattt agcagtcgtt taccggagaa ccagtaatag tggtaacaaa acccgtccac
     1081 gccataccgc ttagccatat caatctgatc gcgtcgtgtc tcgcgcacac gaagatcgta
     1141 gaaaccaaag tcagcgggta aatgcggctg gcgatggcct tcaaacagcg gcttcgcttt
     1201 cgtgacgttc gtccattcag taaacccttt cccccaccag agatcattct caggtatagg
     1261 atgaaactga ggaaggtaaa acgcgatcaa ccgaggtgtc ggaactgtca tgtatagaat
     1321 ctatccggtt caggatggca tttctgcaac ggcggagcag gacttaaaac gattagtcat
     1381 ctgtgacgac agctcatctc tgaaagtcac actgaacgca tacgtttcac aagctgaaat
     1441 ctcagtctac gcgcactctc aataagtgcg tacatgcttc cgcatgacgc tcgtatcaac
     1501 ttgatttggt ctctgttaaa attggcgtcg cctgacgctg accggccctc gtcgttatat
     1561 attgacacga ccactcctgt ataaacgaac ggcacttcat cgcccactaa tcgtaggttg
     1621 tatgcatgat cagcccgcgc tggatattcc aggctataag aatacttcct gtagacggat
     1681 tttggataca tcaatgcctg atgacaaatg ttatggcgca tcaatctgaa ttttgaaaat
     1741 ttaccgccat aaatctttcc actggaccgc ttgataacgt ccccataata gacagtatcg
     1801 gctcgattta agagacttgc catcttacca agagagtcga gcaaaacatc atcgcaacca
     1861 agaaaaatca gccagtctcc cgtcgcaatc gaacacacct tgttcattgc gtcgtatatc
     1921 cccttatccg gctcgcaaat cagcacagtg atgaattggg catattcgtt caggatagct
     1981 tgagtgccat cgtctgaacc accatccaca atcaccaact caatgttctt gtaggtctgt
     2041 ctgataatgc tgtccaacgc atgcctgata tgaccgaccc cgttatacac cacgataccg
     2101 accgatatct taggaggatg gcaattcatt gaagtcaacc aatgatgcgc ggcctacttc
     2161 cgtcttcctc cgccccacgg gctcacaccg tttgtccctt tgaatacgag ctgtgccatc
     2221 ctgctatgca ctgccttgtt cacattctcg cttttacagg cgaggacttc ccgctgggct
     2281 aaaactaccg cgccagatcc ctcttaagaa attcgcggtt ctattcttca tattcaacag
     2341 cgcaacacca tcagaaatta catgctgcca gctcattttc gctgaatcga gctcacggtg
     2401 aagatcgctt aaatcgtacc ccataacctt cagac
//
LOCUS       DASTJP010000409         4079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_550354, whole genome shotgun sequence.
ACCESSION   DASTJP010000409 DASTJP010000000
VERSION     DASTJP010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550354"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /gene="rarD"
                     /locus_tag="VFQ23_11845"
     CDS             complement(<1..822)
                     /gene="rarD"
                     /locus_tag="VFQ23_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HET9907333.1"
                     /translation="MNKGILNGIGAYALWGFFPIYWKLLHHVPALQLLGHRILWSFLL
                     LFGVIFATKQLDEFRATLNMRTFGIYFIAALLIGVNWLTYVWAVNAGYIIETSLGYFI
                     NPLFSVLMGVVFLGERLRFGQWIPVILAAIGVGYLTFAYGQLPWIALLLAFSFGFYGL
                     VKKLAPLGSLQGLTLETGILFLPALIYLGFVQSNDSAAFLHSGITSDFLMVGAGLVTT
                     VPLLMFASAAKQIPLSMIGVLQYLAPTIQFLIGIFIYKEAFDQSRLIGFSIVWLAL"
     gene            complement(912..1295)
                     /locus_tag="VFQ23_11850"
     CDS             complement(912..1295)
                     /locus_tag="VFQ23_11850"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET9907334.1"
                     /translation="MPQFFTLDEANHAIKTIRPLMDEVQTIRRKILASQPEAWPAIEK
                     SAGNGGNRALSAMVQDFEKLDALIHQIQDAGAQIKDINTGLLDFSALKDGREVYLCWQ
                     YGEDDIAFWHEVDAGFAGRQPIELF"
     gene            complement(1361..3802)
                     /locus_tag="VFQ23_11855"
     CDS             complement(1361..3802)
                     /locus_tag="VFQ23_11855"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9907335.1"
                     /translation="MTDTKQLTLPITGMTCANCVATVERNLKKLDGVNTAVVNLSSER
                     ATVEFEPTKLGLPEMIARVQRAGYGVASGEADLVIKRLADDNDARRLEKVLTTLEGVL
                     EAQVNYTAEKARVRYVPTVVSQAEIRAAVSKAGFEAVELGSDAEDVEAQAREHEINEQ
                     RRLLIIGLIFTVPLFIFSMLRDFEILGHWAHESWVNVTMMLVAAPVQFYVGWQYYVSA
                     FKALRNGSANMDVLIAMGSSVAYIYSIAIVLGLVAGHVYFETAAVIITLIRLGKFLEA
                     RAKGRTSEAIKKLMGLRAKAAHVIRSGLEVEVPVDEVRVGDIVLVKPGEKIPVDGVVI
                     EGRSPVDESMLTGESLPVEKKPGDAVIGATLNKLGSLKFEATKVGKETALAQIIKLVE
                     DAQGSKAPIQKLADQVSAVFVPIVIAIALLTFAVWYFIVPQFTNYQFPESPFTTALIN
                     MVAVLVIACPCAMGLATPTAVMVGTGKGAELGILFKNSEALERAGNVNVVVLDKTGTI
                     TKGQPAVTDIINNSKLPTDDLLRLAASVEKGSEHPLGEAIWAEATTRGLNLAEATGFK
                     AEPGHGVQAEVEGRSVVVGNMRMMNARGYSMNGLEPEVARLQSEAKTAMLVAVDGEVY
                     GVIAVADTVKDGSQAAISELHQMGLQVAMITGDNQKTAEAIAKQVGIDQVLAEVLPEG
                     KSSEVKKLQGENGQPSAINVVAMVGDGINDAPALAQADVGIAIGTGTDVAIATAPVTL
                     ISGDLRAVARAISLSRKTLATIKQNLFWAFFYNVILIPAAAFGYLNPMLAAGAMAFSS
                     VFVVTNSLRLRKSRI"
     gene            complement(3862..4044)
                     /locus_tag="VFQ23_11860"
     CDS             complement(3862..4044)
                     /locus_tag="VFQ23_11860"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HET9907336.1"
                     /translation="MTTTFKDPVCGMDVSPDEAAGTSEYKGVTYYFCAPGCKRSFDKD
                     PEKYIGTNEETQDHSS"
BASE COUNT         1074 a         1089 c          908 g         1008 t
ORIGIN      
        1 cagggcgagc cagacgatac tgaaaccgat caagcgcgac tgatcgaatg cttccttata
       61 gatgaaaatg ccgatgagaa attgaatcgt cggcgcgaga tattgcaata caccgatcat
      121 cgaaagtgga atttgttttg ccgcagatgc gaacatcaga agcggaactg tcgtgacaag
      181 accggcgcca accataagaa aatcagatgt gatcccagag tggagaaacg cagcggaatc
      241 attggattgt acaaatccaa ggtagataag tgctggtaaa aagagaatgc cagtttcaag
      301 cgtgagtccc tgcaacgaac cgagaggtgc cagctttttt acgagtccgt aaaagccaaa
      361 tgaaaacgcc agcaaaagtg caatccaggg tagctgacca taggcaaacg tcaggtatcc
      421 aacgccaatt gcagcgagta tcaccggaat ccattgacca aaacgaaggc gctcgcccag
      481 gaaaaccacg cccatcaaca cactgaatag tggattgata aaataaccca ggctggtttc
      541 aataatataa ccggcattga cagcccacac gtacgtcagc caatttactc caatgagaag
      601 cgccgcaata aaataaatgc caaacgtgcg catgttcagc gtagcacgaa actcatccaa
      661 ctgtttagta gcaaaaatca caccaaaaag caacaggaat gaccacagga tacgatgccc
      721 cagcaattgc aaagctggga catgatgcag aagtttccaa taaatcggaa agaaccccca
      781 taacgcatac gcgccaattc catttaggat acctttattc attattttcc tttgaaatgt
      841 taaacatagg aagcactcgg agtagacgcg gcagagccaa atctactcgc actacgatgt
      901 tgttttatta atcaaaagag ttctattggc tggcggcctg caaaccccgc gtcgacctcg
      961 tgccagaaag caatatcatc ctccccatat tgccagcaca aataaacttc tcttccatct
     1021 tttagtgcag aaaaatccaa taagccggta ttgatgtctt tgatttgcgc gcccgcatcc
     1081 tgaatttgat ggatcagtgc atccagtttt tcgaaatcct gcaccatagc acttaaggcg
     1141 cgattcccac cattccctgc ggatttttca atggcaggcc aggcttcggg ttgacttgcg
     1201 aggattttcc ggcgaattgt ctgaacttca tccattaatg ggcggatggt tttaatggca
     1261 tgattcgctt catcaagagt aaaaaattgt ggcatgatcg ttgtatagtt cgagtataca
     1321 ccaaataaaa aaggacgacc ttttcggtcg tccctgagat tcagatcctt gattttctca
     1381 atcgcaaact gtttgtcacc acaaaaacac tactgaatgc catcgctcct gcagcaagca
     1441 tggggttcaa gtatccgaat gctgcagcag gtatcaggat gacattgtag aagaatgccc
     1501 agaacaaatt ctgcttgatg gtagcaagtg ttttccgaga gagagaaatg gcgcgggcta
     1561 ctgcacgcag gtcaccacta atcaacgtca ctggcgcggt agcgattgcc acatcggtgc
     1621 cggtcccaat tgcaataccc acgtctgctt gtgcgagcgc gggagcgtca ttgatcccgt
     1681 cccccaccat agcaactaca tttatagctg atggttgccc attttcgccc tggagtttct
     1741 tgacctcgct cgattttcct tcaggcagga cttcagccaa tacctgatca atgccaactt
     1801 gtttcgcaat ggcttcagcc gttttttggt tatctccagt gatcatggca acctgtaagc
     1861 ccatttgatg caattcacta atcgctgcct gtgaaccatc ctttacggtg tctgcaaccg
     1921 caattacacc ataaacttct ccatccactg cgacgagcat ggcagtcttg gcttcggatt
     1981 gcaggcgtgc tacttcgggt tcgagaccgt tcatggagta cccgcgcgca ttcatcatgc
     2041 gcatgttgcc taccacaaca cttcgtcctt cgacttctgc ctgcacgccg tgcccaggct
     2101 ctgctttaaa ccctgtcgct tctgccagat tcagacctcg cgttgtcgcc tctgcccaga
     2161 tcgcttcgcc aagagggtgt tcacttcctt tttcgacact tgccgcgagt cgaagaagat
     2221 catcagtggg aagtttggaa ttattaatga tgtctgtaac agcaggttga cccttcgtaa
     2281 tcgttcccgt tttatccagc acgaccacat tgacgtttcc tgcacgctcc agcgcttcac
     2341 tattcttgaa tagaattcct aattctgcac ccttgccagt ccccaccatc accgccgtgg
     2401 gtgtagcaag gcccatggcg cagggacagg caatcaccaa aactgctacc atattgatca
     2461 aggcggtagt gaagggtgac tcaggaaatt ggtaattcgt aaattgagga actataaaat
     2521 accagacagc gaatgtaagt aaagcaatgg caattacgat cggcacaaac accgcggaaa
     2581 cctgatccgc aagtttttgg atcggagcct tgcttccttg tgcgtcttcc accaatttaa
     2641 tgatctgggc cagcgcggtt tctttgccaa cttttgttgc ttcaaatttc aggctaccca
     2701 gcttattcaa ggtcgcgccg atcacagcgt caccgggctt cttttcgact ggcaaggact
     2761 cacccgtcaa catggattca tcaactgggc ttcgtccttc gatcacgacg ccatccacag
     2821 gaattttctc gccaggtttc acgaggacaa tgtcgcccac gcgcacttcg tccacgggca
     2881 cttcaacctc aagtccagat cgaatgacgt gagcagcctt cgcacgcaat cccattaact
     2941 tcttgattgc ttcgctcgtg cggcccttcg ctcgggcttc caaaaatttt ccgagtcgaa
     3001 ttaaggtaat gatgaccgcc gccgtctcaa aatacacatg accagcaacc aaccccagca
     3061 caatggcgat cgaataaata tacgctaccg atgaacccat tgcaatgagc acatccatat
     3121 tggcggatcc attgcgcaaa gccttgaacg cgctgacata atactgccag cctacataga
     3181 attgaacagg ggcggcaacc agcatcatcg taacattgac ccagctttcg tgcgcccagt
     3241 gtcccaggat ctcgaaatca cgcaacatcg aaaagatgaa cagcggaaca gtaaagatca
     3301 agccaatgat cagcaaacgg cgctgttcat tgatctcatg ttcgcgcgcc tgagcttcaa
     3361 cgtcttcagc atcgctgccc aattccacgg cttcaaatcc tgcctttgaa acggcggctc
     3421 gaatttctgc ctggctaacg acggtaggca catatcgtac gcgcgctttc tcagctgtgt
     3481 aattcacctg cgcttccaat acgccctcga gggtcgtcag caccttctca agacgtcgtg
     3541 catcattgtc atctgccaga cgcttgataa caaggtcagc ttccccactc gccactccat
     3601 agccagcgcg ttggacgcgc gcaatcatct ccggaagacc cagtttggtg ggctcgaact
     3661 cgaccgtggc gcgctcagat gataagttga caacggccgt gttcacacca tccaattttt
     3721 tcaggttgcg ttcaacggtc gcgacgcagt ttgcacaggt catgccggta ataggcagag
     3781 ttaattgttt tgtatcagtc ataatgttcc agtttcggct tttttcggcg cgaatataac
     3841 tgcgccaact caaggataag tttaagacga atgatcttgc gtttcctcat tcgtaccaat
     3901 atatttctcg gggtctttgt caaatgaccg cttgcagcca ggggcgcaga agtagtaggt
     3961 cacgcctttg tattcagagg tacctgcagc ctcatccggg ctgacatcca taccacatac
     4021 ggggtctttg aacgttgttg tcattattta ttctccttag ataagttagt agttcaatt
//
LOCUS       DASTJP010000410         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_86479, whole genome shotgun sequence.
ACCESSION   DASTJP010000410 DASTJP010000000
VERSION     DASTJP010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86479"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VFQ23_11865"
     CDS             <1..566
                     /locus_tag="VFQ23_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907337.1"
                     /translation="VFQMGKGYRDDTGGQELYQLSVEAYEKCLSLKPNDAQWHAGFAD
                     LLAERAMWDSWFGDPTPEAFRALEEIHTALELAPNDPIVREIAQNITYLIPDAITQNG
                     DSFDFPWLTATPTALPPTPTMPPPEESTPTPPTSPAATETSPAQPTPVGPTQTNPAPV
                     PEASNPLCGSAGLLPLIAIIWFVWKRR"
     gene            652..1236
                     /locus_tag="VFQ23_11870"
     CDS             652..1236
                     /locus_tag="VFQ23_11870"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HET9907338.1"
                     /translation="MQNPLNLPVIVLFILTACSSTRDVSTKPTALPQPTATRVFPTPS
                     APGDPVLWRTLRVSMDQPEITDTFINEFGSQRVPSAGQKFLWIHLTLENVGTEETLLP
                     EPENFSVLYAESEIKPIYGHRQEYADYSDSGATLFPGQELDAWLRFDIPAAADLSELW
                     FVFLPTSSQVGASPSSPNYPYAENKPTYVWKCEP"
     gene            1447..1665
                     /locus_tag="VFQ23_11875"
     CDS             1447..1665
                     /locus_tag="VFQ23_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907339.1"
                     /translation="MKRHGCLVTYLLYTAIYYIYFILIGKFLSSLSLGIQSDDLLAFT
                     LLMSSLLALATAILVMNWKRILSLLKLK"
     gene            1783..>2430
                     /locus_tag="VFQ23_11880"
     CDS             1783..>2430
                     /locus_tag="VFQ23_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907340.1"
                     /translation="MDTESKIRTSYTTEYVVITGYVLFFCILIFACLGFFYINRSAYF
                     PKPPAANEFAEALPPTTPTPHITSDHQFEKDSILFQEDFNVNQNHWLGGDNDTKERLQ
                     GGQLFFQSAYEGNYAIISCESCPYLDTPYYLEASLSTSMATDESYGIIFNRSYTLDDF
                     FLFMINPESMNYSLFHHTQDGWTHRASGASSQIQPYPESTTLGVYANKDSLELYIN"
BASE COUNT          599 a          660 c          549 g          622 t
ORIGIN      
        1 aagtgttcca gatgggcaaa ggttatcgtg acgatactgg tggacaggag ttataccagt
       61 tgagcgttga ggcatacgag aaatgcctgt cgctcaagcc gaacgacgcc cagtggcacg
      121 ccggctttgc cgatctgctg gcagagcgcg ccatgtggga ctcatggttt ggtgacccga
      181 ctcccgaagc attccgcgcc ctcgaggaaa tccacaccgc gctcgaactg gcgcccaatg
      241 acccgatcgt tcgagagatc gctcaaaata ttacttatct gattcccgac gcgattactc
      301 aaaacggaga tagcttcgac ttcccctggc taaccgcaac gccaactgcg cttccgccca
      361 cgccgaccat gcctcctccg gaagaatcaa ctcccacgcc tccaacctcg cctgccgcga
      421 ccgagacatc tccagcgcag cctactccgg tgggccccac gcaaacgaat cctgcacctg
      481 ttcccgaagc atcgaatccc ctttgtggat ctgccggact cctgccattg attgctataa
      541 tttggtttgt ctggaaaaga agataaagga tgctctagga aaccaattcc catcaattga
      601 cggggaaacg gatacgctcg tccatacgta tgaagagtgg atgtgtttcc tatgcaaaat
      661 ccactcaatc ttccagtcat tgttttgttc attttgactg cctgctcttc cacacgcgat
      721 gtatccacca agccaacagc tcttccgcag cctacggcga ccagagtttt cccgacaccc
      781 tccgcaccag gcgaccctgt cctctggcgt accctgcggg tgagcatgga tcagcccgag
      841 atcaccgaca ctttcatcaa tgaatttggc tcccaaaggg tgccttccgc cgggcaaaaa
      901 tttctctgga tccatcttac actcgaaaat gttggaaccg aggagaccct tttacctgag
      961 cctgaaaatt tcagcgtgct gtatgccgaa tccgagatta agcctattta tggccatcgc
     1021 caggagtacg ccgactattc cgattcaggc gccaccttgt tcccagggca ggagctggat
     1081 gcctggctgc ggttcgatat tcctgctgct gcagatttga gcgagttgtg gtttgttttc
     1141 ctgcctacga gttcgcaggt gggcgcctcg ccatcttccc cgaattatcc ttatgcagag
     1201 aataaaccga cgtatgtgtg gaaatgtgag ccctgacttt agatggggct tcgtaacctc
     1261 tcaggaccca ggcatttttt gaatgtgcgt caaccgaagg tctacgacca agggtcatgc
     1321 tctgattcac ccgaaagcga agataaaaat cacttcccac cagtgctctg ctatgctagc
     1381 atgttggcgc tgtttgggat gtaatcacag cccggctagc gtggagcatc acaaaggttg
     1441 ttttggatga aacgacatgg ttgtctggtg acgtatttac tatacacagc catatattac
     1501 atctatttca ttttgatcgg caaatttttg tcaagcctat cgctgggaat tcagtctgat
     1561 gacttgcttg cctttacgct tttgatgtct tccttgctgg ctcttgcaac agcgattctg
     1621 gtgatgaatt ggaaaaggat tttgagcctt ctgaagttga aataaaggcc gacaacccat
     1681 tcacctcctg ctgcttctga ttgcgtccag ccttcatttg cgacaggtaa aaccaactgc
     1741 atatcaagat tctcactgct gaacactgac aggtggaagt caatggacac tgaatcgaaa
     1801 ataagaacaa gttacacaac agaatatgtt gtgataacgg gatacgtact tttcttttgt
     1861 atattaatat ttgcctgcct tggatttttt tacatcaaca ggtctgcata ttttccaaaa
     1921 cctcccgcgg caaatgagtt tgcggaggct ctgccaccca caaccccaac accccatatc
     1981 acgtccgatc atcaattcga gaaagactcg atcctgtttc aggaagattt caacgtcaat
     2041 caaaatcact ggcttggcgg ggacaatgat acaaaagaaa gacttcaggg cggccagcta
     2101 ttttttcagt ccgcgtacga ggggaactac gcgatcataa gctgtgaatc ctgcccctac
     2161 ctcgatacgc cctactacct tgaagccagt ctctccacca gcatggcgac agatgaatca
     2221 tatgggatca tattcaatcg cagctatacg cttgatgatt tttttctctt tatgatcaat
     2281 cctgaatcca tgaactattc tttatttcat cacacgcaag acggttggac tcatcgtgcg
     2341 tccggcgcaa gcagccagat ccagccttat ccggaaagca cgacacttgg ggtgtatgcc
     2401 aacaaggatt cactggaact ttacatcaat
//
LOCUS       DASTJP010000411         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1050488, whole genome shotgun sequence.
ACCESSION   DASTJP010000411 DASTJP010000000
VERSION     DASTJP010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050488"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFQ23_11885"
     CDS             <1..381
                     /locus_tag="VFQ23_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET9907341.1"
                     /translation="GYCHLRQDLRSFRLDRITDIETLNAHFKRPEGFDPLAHMMQAIA
                     TMPRKYPFELLLKTDINTAHKEVFDVLGVLEPLKDGVIMRGSVEDLDWLARQISIFSF
                     DFIVLAPEELKAELRMHATKLAAV"
     gene            complement(459..866)
                     /locus_tag="VFQ23_11890"
     CDS             complement(459..866)
                     /locus_tag="VFQ23_11890"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9907342.1"
                     /translation="MSKNPSVKDWLANLTPELRAITKPLRAMARKQMPGTHEFIYHDA
                     IGYSVSDSPFDRICYIAPQKKSYVNFGFFFGAGLPDPKKLLIGEGKRLRHIKVWSVDD
                     AKNPALAKLVKATWKSAPESISKIRPGRKQTQA"
     gene            complement(899..2185)
                     /locus_tag="VFQ23_11895"
     CDS             complement(899..2185)
                     /locus_tag="VFQ23_11895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9907343.1"
                     /translation="MRKFVIIWIGQLISTIGSGLTGFALSIWIFQQTGQAAPFVYTAF
                     FNSLPVVLFSLFAGALVDRLNRRWVMILTDVGAALTTLGIFLLYTSGNLEVWHIYVSS
                     FFASLFATFQLPAFTASITLLVPKEHLTRANGMVQTGGSLEGLLAPLLASLLVGLIGV
                     GGMFLIDFVTFFAAVGTLLLVRIPQAEATEEKKAQKILQDIKQGWSFLSARPGLVALA
                     GYIGIVNILSTSVIALTTPLILSFSNERTLGLTQMISSLGLLLGGVLISSWGGTKRKM
                     TGIYLGVLLGGLGLMFGGLRSVFWWIALGIFFFLFPIPTVNAQLRSIIQVKSPPDLQG
                     RVFSVVFMIARLGPPIGFLISAPLADRIFEPGMLPGGSLASVFGPLFGVGAGRGVGLM
                     MSLAGVGFWLTTALMYSYPRLRQIEDELPDVVSAPE"
     gene            complement(2306..2794)
                     /locus_tag="VFQ23_11900"
     CDS             complement(2306..2794)
                     /locus_tag="VFQ23_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907344.1"
                     /translation="MRAILQQIIEDSRYLKNIEYGEPRPGHPEGKVKYHIAEMEENLE
                     VLRSRGISDEQHWKLKFLIHVHDCFKAEAIPNSAILNPNSHASLARKFASEFTHDSDL
                     LNMIQYHDVNFALWKQLKSTGRYDVERFSTLLQEIKDWDLFLLFLILDGSTKGKDPEK
                     VR"
     gene            complement(2804..>3252)
                     /locus_tag="VFQ23_11905"
     CDS             complement(2804..>3252)
                     /locus_tag="VFQ23_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907345.1"
                     /translation="DDEELASYLSMLRQTVQMANGIDLWTLAHHLQKKMQSSISAGDH
                     FVAAQFAESLMQFITERKAFRLSATALNYNEAVTIDAGYGKIKVTAVHGFPPIFDLGP
                     ELFAQAHIFNNQLFMDFAYLEADMTRDESQAILEEIRTVLAHAVGK"
BASE COUNT          900 a          813 c          735 g          804 t
ORIGIN      
        1 ggatactgcc atctgcgcca ggatttacgc tcgtttcgac tggatcggat tacagatata
       61 gaaactctga atgcgcattt caaacgcccg gaaggttttg atccgctggc tcacatgatg
      121 caagcaattg caaccatgcc gcgtaaatat cccttcgaac tccttctcaa aacggacatc
      181 aataccgcgc acaaagaagt ctttgacgta ctgggtgttt tagagccact gaaagatggg
      241 gtgatcatgc gaggcagcgt ggaagacctc gattggctgg caaggcagat atcaattttc
      301 tcatttgact ttatcgtatt agcaccagag gaattgaagg cggaactaag aatgcatgca
      361 acaaaattgg ctgcggtgtg aaacatgtct ttccaaagac ttcacctcta aatttcgttc
      421 agcgcgaaat tgagtgtgtt tcccggggtg attgcttctt atgcttgggt ttgtttccgt
      481 ccagggcgga ttttgctaat cgattcagga gcacttttcc aggttgcctt gacgagtttt
      541 gccagcgcgg gattttttgc atcatccacg ctccagactt tgatatgccg caaacgtttt
      601 ccttctccaa ttaatagctt tttagggtca ggcaaacccg cgccaaagaa aaagccgaaa
      661 ttcacataac tctttttctg gggagctata tagcatatcc ggtcaaacgg cgaatcgctg
      721 acagaatatc caattgcatc gtgatagata aattcgtggg tacccggcat ttgttttctg
      781 gccatggcgc gaagtggctt tgtaattgct ctcaactcgg gagtcaaatt cgcgagccaa
      841 tctttgacgc ttggattttt actcattcgt gtgctccgtt gaaacacatt tcccaaggtc
      901 attccggcgc cgaaacgaca tcaggcaatt cgtcttcgat ctgtcgcaga cgcgggtagg
      961 aatacatcag ggctgtggtc aaccagaaac cgacccctgc cagactcatc atcagcccaa
     1021 cgccacgccc tgctcccacg ccgaataacg gcccaaatac agatgccaga gaacctcctg
     1081 gcagcatccc gggttcgaat atccgatcgg ccaaaggcgc ggaaatcaag aacccgatgg
     1141 gaggtcccag ccgcgcgatc ataaagacaa ctgagaacac ccggccctgt aaatcaggcg
     1201 gtgacttgac ctggatgatg gaacgcaatt gtgcattcac ggttgggatt ggaaacagaa
     1261 agaaaaaaat tccaagagcg atccaccaga aaaccgaacg cagtccgcca aacatcaatc
     1321 ccagtccacc gagaagcacg ccaagataga taccggtcat cttgcgtttt gtaccgcccc
     1381 aggaactgat caaaactcct cccagcagca ggccaagact cgaaatcatt tgagtcagcc
     1441 ctaacgttcg ttcattggaa aatgacagaa ttaagggtgt cgtcaaggca attacagacg
     1501 tggaaagaat atttacgatg ccaatatatc ctgccagggc caccaacccg ggtcttgcag
     1561 aaaggaaact ccacccctgt ttaatatcct gaagaatctt ctgagctttt ttctcttcag
     1621 tggcttctgc ctgaggaatg cgaaccagta acaaagtacc aactgctgca aagaatgtca
     1681 caaaatcaat caggaacatc ccgccgacgc caatcagacc gaccaataaa ctagccaaca
     1741 gtggtgcaag caagccttcg agagatcccc cggtctgaac cattccattt gcgcgcgtca
     1801 gatgttcctt tggcaccaac agagtgatcg atgcggtgaa agccggtaac tgaaatgtgg
     1861 caaacagaga tgcaaaaaat gaagacacat aaatatgcca gacttccaga tttccgcttg
     1921 tgtaaagaag aaaaattcca agagttgtca gcgccgcgcc tacatcagtg agaatcatca
     1981 cccaacgacg gttcaaacga tcgaccaacg cgcctgcaaa taaactaaat agtacaaccg
     2041 gcaacgaatt aaagaaggca gtatacacaa agggagcagc ctgcccggtc tgctgaaaaa
     2101 tccaaatgga aagagcaaaa ccggtcaggc ccgaaccaat ggtcgagatt aattggccga
     2161 tccaaataat aacgaatttg cgcatggatg cctcaaagtg aattcttagg gattaatctg
     2221 ctccctccta accgatattt acgaattata taacccagct ttcatcgatt cgggtttctt
     2281 tgtatttctt cacttcctta atgaactagc gaactttctc aggatctttt ccttttgtac
     2341 tgccatccaa aattaggaac aggaggaaaa gatcccaatc ttttatttct tggagcaagg
     2401 ttgaaaatcg ctcgacgtca taacgccctg tgctctttaa ctgtttccaa agtgcaaagt
     2461 taacatcatg gtactggatc atattgagta aatcactgtc atgtgtgaat tctgaggcga
     2521 atttcctggc cagagacgca tgactgttcg ggttcaggat tgccgagttc ggaattgctt
     2581 ctgccttgaa gcaatcatgt acgtggataa gaaatttcaa tttccaatgc tgctcgtccg
     2641 agataccgcg cgatcgtaga acttccagat tttcttccat ctctgcaatg tgatatttaa
     2701 ctttcccctc ggggtgcccc ggacgcggct caccatattc aatgtttttc agatatcgag
     2761 aatcttcgat gatctgttga aggatggctc tcattcgatt gcttcatttc cccactgcat
     2821 gggccagaac ggttcggatt tcctccaaaa tcgcctggga ctcatcgcga gtcatgtcgg
     2881 cctcgagata ggcaaaatcc atgaacaact gattgttaaa gatatgagcc tgcgcaaaga
     2941 gttcaggtcc cagatcaaag ataggcggaa agccgtgcac ggcggtaact tttatctttc
     3001 catagccggc atcgatcgtc acagcctcgt tgtagttcaa cgcagtcgcg ctcaagcgaa
     3061 atgcctttcg ttccgtgatg aactgcatca gcgattctgc aaactgcgca gccacaaaat
     3121 gatcaccagc actgattgag ctttgcattt tcttttgcag gtgatgggcc agcgtccaca
     3181 ggtcgatgcc gtttgccatc tgaactgtct gccgaagcat ggagagatag ctggcaagtt
     3241 cttcatcatc ca
//
LOCUS       DASTJP010000412         4020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1391474, whole genome shotgun sequence.
ACCESSION   DASTJP010000412 DASTJP010000000
VERSION     DASTJP010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391474"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VFQ23_11910"
     CDS             complement(<1..1145)
                     /locus_tag="VFQ23_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET9907346.1"
                     /translation="MSETFEFPKGFLWGTATAAHQVEGNNTNSDIWLMEHAPESPYVE
                     ASGDACDHYHRYRQDIALLAELGFNSYRFSIEWARIEPEEGEFSLAVLEHYRDVLRAC
                     HEHQLTPIVTFHHFSSPRWLIASGGWESADTPDKFARYCERATRYLGDLIGAACTINE
                     ANIASLAAEWGWLPPARQLHKAAWWAGTAKSLGISPRRLSPFMYATSSKATEVILTAH
                     RRAVEAIKSASSAFPVGITLALQDIQAAEGGERRAARMRKVINDIYLESLNGDDYVGV
                     QIYSRVRSGPLGVLPPEDGIELTQMGYEFWPEALEAAIRDAIAKSGLPVIVTENGIAT
                     TDDTRRIAYVQRALQGVINCLNDGLDVRGYTYWSAFDNYEWSLGYRPT"
     gene            complement(1175..1825)
                     /gene="pgmB"
                     /locus_tag="VFQ23_11915"
     CDS             complement(1175..1825)
                     /gene="pgmB"
                     /locus_tag="VFQ23_11915"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HET9907347.1"
                     /translation="MIRAFIFDLDGVITDTAEYHYRGWKRLADEEGLSFTREDNEHLR
                     GISRRESLMMIIKDKVYSEEKIQEMMEHKNGYYLDFIKEVTPRDLLPGAKELLEEIRT
                     AGLKNALGSASKNAGEVIERLGIRHLFDAISDGHSVERQKPAPDLFLHAARQLDLTPA
                     DCVVVEDAAAGIEAARAGGFRSVGLGPGERVGQADALFPSLAGVRLSDLLKIFDLQ"
     gene            complement(1822..3246)
                     /locus_tag="VFQ23_11920"
     CDS             complement(1822..3246)
                     /locus_tag="VFQ23_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET9907348.1"
                     /translation="MVTPRPRYHFTPPQNFMNDPNGLVFYDGEYHLFYQHNPFGNVWG
                     HMSWGHAVSTDLIHWEHLPVALVEDDQGMIFSGSAVVDWQNTSGFGNTDQPPLIAIYT
                     SHSETEQTQNIAYSLDHGRTWTKYEGNPVIAIGARDFRDPKVFWHESTKRWIMVSALS
                     DQHKIRFDSSPDLKHWTHLNDFGPAGAVDGMWECPDLFPLPVENAPKKIKWVLKVDAL
                     KGTGGQYFVGDFDGTRFIHDGPDDQILRLDYGNDFYAAQSWSDIPEVRRIWVAWLNHW
                     NYANLIPTSLWRGLFSIPREVQLRQYPEGVRLIQKPIEELKGLRKSIFHATESDIQRI
                     NSQITRLELDNSLELYLEFSVERGSQVGIKLGMGEAEETVIGYDSEAQELFLDRSQAG
                     DSSFSSEFAAVHRASLAPEQGKISLSIFVDSCSVEVFANDGRTVISDLIFPTTQNTHL
                     QLFASHGDIQINKIDIWSLVIENK"
     gene            complement(3293..>4020)
                     /locus_tag="VFQ23_11925"
     CDS             complement(3293..>4020)
                     /locus_tag="VFQ23_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9907349.1"
                     /translation="YSLFLNCKGTGRPTVILDSGLDSDSNAWLTVMPRVQGSARICAY
                     DRAGLGRSDPAPKTPRTSMDMVVDLHTLLNNAHLGGGYVLVGASIAGFNALLYTNQYP
                     QEVVGIVLVDASHPDQFARLLAALPPQSPDESTELKDLRNSLSVPIQSIEGMDVETSA
                     EQVRATGSLGNLPLTVLTAGSPSLSEIDIGLGEVWLEMQMELAGLSANSAHIVVPDAT
                     HCIQCDAPEAVADAILNVVNSAR"
BASE COUNT          965 a         1049 c         1000 g         1006 t
ORIGIN      
        1 gtggggcgat agcctaagga ccattcataa ttatcaaagg cggaccaata cgtatagccg
       61 cggacatcga gtccgtcatt caaacaattg atcacgccct ggagtgcacg ctgcacgtac
      121 gcgatgcgac gtgtatcatc cgttgtagcg atgccgtttt cagtcacgat tacgggaagt
      181 ccactttttg caatggcatc cctgatcgca gcttcgaggg cttccggcca gaattcgtat
      241 cccatttgag tcagttctat gccatcttct ggtggcaata cgccaagtgg accggagcgc
      301 acacgagagt agatttgtac gccaacataa tcatctccat taagagattc cagatagata
      361 tcattaatga ctttacgcat ccgcgccgcg cgtctttcac caccttcggc agcctggatg
      421 tcttgaagtg ccagggtaat cccaactggg aacgcgctgg aggcagactt gattgcctcc
      481 accgcgcggc ggtgagcagt tagtatgacc tctgtcgctt tggatgacgt ggcatacatg
      541 aacggagata agcgccgagg tgagattccc aaggattttg cagtgccggc ccaccacgct
      601 gctttgtgga gctgccgcgc aggaggcagc catccccatt ctgcagccag cgaggcgata
      661 ttggcttcat tgatcgtaca tgccgcgccg ataagatcac caagatatcg agtggcgcgt
      721 tcacaatagc gggcgaattt gtccggtgtg tccgcgcttt cccagccgcc ggaagcgatc
      781 agccagcgcg gtgaactgaa atgatgaaac gtgacgatcg gcgtaagctg atgctcatga
      841 caagctctga gcacatctcg atagtgttca agaactgcca gcgagaattc accttcttcc
      901 ggttcgatgc gcgcccattc aatggaaaag cgatacgaat tgaatccaag ttcggctaac
      961 agtgcaatat cctgtcgata acgatgataa tgatcgcagg catcaccaga agcttccacg
     1021 tacggcgatt cgggcgcatg ctccatcaac catatatcgc tgttggtatt gtttccctcc
     1081 acctgatgtg ctgcggtcgc ggtcccccag agaaagcctt tcgggaattc aaatgtttcg
     1141 ctcatttcat ctcctctttg agaaccggct ctggctattg caaatcaaaa atcttcaaca
     1201 ggtcagataa gcgaacgcca gcaaggctgg ggaacagcgc atcggcctgt ccgacgcgct
     1261 cgcccggacc cagtcccacg gaacgaaatc cgccggcgcg cgcggcctcg atgcctgccg
     1321 ccgcatcttc gacgactaca caatccgcag gagtcagatc cagttgacgg gctgcatgta
     1381 gaaaaagatc aggcgccggt ttttgacgtt ccacgctatg accatctgat atcgcatcaa
     1441 acaaatgtcg tatgccaagc cgctcgatca cttcccctgc atttttgctg gcggaaccca
     1501 acgcgttttt caaacctgcc gtacggattt cttccaacag ttccttggcc ccgggtaata
     1561 gatcgcgggg cgtaacttct ttgatgaagt ccaaatagta tccgttcttg tgctccatca
     1621 tttcttgaat tttttcttcg gaataaactt tgtctttgat aatcatcatt agcgattcgc
     1681 gccgcgagat tccgcggaga tgttcgttat cttcgcgcgt aaaggacagt ccttcttcat
     1741 ctgccaaacg cttccagccg cgatagtgat actcggctgt gtcagtgatc acgccatcca
     1801 ggtcaaagat aaatgctcgg atcatttgtt ctcgatcacc aaactccaga tgtcaatctt
     1861 gttgatttga atgtctccgt ggctggcaaa aagttgcaga tgtgtatttt gagtcgtggg
     1921 aaaaatcaaa tcactaatga cggtgcgtcc atcgttcgcg aaaacttcga ccgagcatga
     1981 gtcaacaaat atactcaggc tgattttacc ttgctccggt gcaagagaag cacgatgcac
     2041 agcagcaaat tcgcttgaaa agctactgtc acccgcctga ctccgatcta aaaacaattc
     2101 ctgtgcttca gagtcataac cgataaccgt ttcttcggct tcgcccatgc caagtttgat
     2161 tccgacttga ctgcctctct ctaccgaaaa ttccaaatat agttccagag aattatcgag
     2221 ctcaagccgg gtaatttgag aattgatcct ttgaatatct gattcagtcg catggaaaat
     2281 tgacttacgc agccctttca attcttcaat cggtttttga attaacctaa cgccttcggg
     2341 atattgcctc aactgtactt cacgcgggat agagaagagt ccgcgccaga gtgaggtggg
     2401 gatgaggttg gcataattcc aatggttcaa ccatgcgacc cagatgcgac gcacttcagg
     2461 gatatcgctc caggattgtg ctgcgtaaaa gtcattgcca tagtcgaggc gcaggatctg
     2521 atcatccggg ccatcatgga tgaatcgagt gccgtcgaaa tcgcccacga agtattgccc
     2581 gcctgtgcct ttaagtgcat ccacctttag gacccatttt atttttttcg gagcattttc
     2641 caccggaagg ggaaacagat cagggcattc ccacattcca tccactgcgc ccgccggacc
     2701 gaaatcgttt agatgagtcc aatgcttgag gtccggtgaa gaatcgaacc ggattttgtg
     2761 ctgatctgac aacgcagaga ccatgatcca gcgttttgtt gattcatgcc agaagacttt
     2821 ggggtctcgg aagtcgcgtg ctccaatcgc aatcaccgga ttgccttcat attttgtcca
     2881 cgttcggccg tgatccaaac tataggcaat gttctgtgtt tgttctgttt cagagtggct
     2941 ggtataaatt gcaataagag gtggctggtc ggtattgcca aacccggatg tgttttgcca
     3001 atccacgacc gcactgcctg agaagatcat cccctggtca tcctcgacca gcgcgacagg
     3061 caaatgttcc caatgaatga gatcggtgct gactgcatgt ccccaactca tatgtcccca
     3121 tacattgccg aatggattat gctggtagaa cagatgatat tctccatcgt aaaataccaa
     3181 cccgttcggg tcgttcataa aattctgcgg cggtgtgaaa tgataacgag ggcgcggcgt
     3241 gaccatgatc agcgccgtag attgtggaga attcgattgg acattgagtc gcttaccgtg
     3301 cggaattcac gacattgagg attgcatcgg ccacagcctc tggcgcgtcg cattgaatgc
     3361 aatgagttgc atctggtacc acgatgtgag cggaattggc tgaaagacca gcgagttcca
     3421 tttgcatttc cagccagacc tcgcccaacc caatgtctat ttcagataaa gatgggctcc
     3481 ctgccgtgag gacggtcaat ggaagattgc ccagcgagcc cgtcgctcgc acctgttctg
     3541 cacttgtctc gacgtccatt ccttcaatgc tttgaatcgg aacggaaagc gagtttctca
     3601 gatcttttaa ttcggtgctt tcgtcgggtg attgcggagg cagcgcagcc agcaatcggg
     3661 caaactgatc gggatgtgag gcatccacca gaacgattcc gacaacttct tgtggatact
     3721 ggttggtata gagtagggcg ttaaagcctg cgatcgatgc tcctaccaat acatatcctc
     3781 caccgagatg ggcattgttc aatagagtat gcaggtcaac taccatatcc atgctggtgc
     3841 gcggtgtttt aggagcggga tcacttctac ccagacccgc gcggtcatag gcgcaaattc
     3901 gcgcagagcc ctggactctt ggcatgactg tcagccaggc attcgaatcg gagtccagtc
     3961 ctgagtcaag aattacggtt gggcggcccg tacctttgca attaaggaag agactatatc
//
LOCUS       DASTJP010000413         8325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_61329, whole genome shotgun sequence.
ACCESSION   DASTJP010000413 DASTJP010000000
VERSION     DASTJP010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61329"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /gene="treS"
                     /locus_tag="VFQ23_11930"
     CDS             <1..1393
                     /gene="treS"
                     /locus_tag="VFQ23_11930"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET9907350.1"
                     /translation="DYYNVAETFGTLEDFKTLIQEAHNRNIRLIADLVLNHTSDEHPW
                     FKAARADRNSPYRDYYVWSDTDQKYQDARIIFLDTEPSNWTWDEVAGQYFWHRFYSTQ
                     PDLNFDNPKVQEEMINIARFWLDLGIDGFRADAVPYLFEREGTNGENLPETHVYLKKL
                     RAFMEQNYPGRILLCEANQWPEDVRPYFGDGDEFHIGFHFPIMPRIFMALKKERADDM
                     IDILNRTPAIPENCQWCIFLRNHDELTLEMVTEEERQWMWQQYAPEPRMKLNLGIRRR
                     LAPLLDNDRRKIELINSLLFTLPGSPIIYYGDEIGMGDNIELFDRNGVRTPMQWDDSE
                     NAGFTTGKPFAEVIRGDFGFEKVNVASQLADKSSVFYAIKKLIQIRKQYRAFGRGTME
                     WVETGNPAVAAYLRKYESESLLILSNLSGSPQTINLPSEHHRDATDLLTEIQVRLEAT
                     LELPPYSYHWLKQ"
     gene            1431..3419
                     /locus_tag="VFQ23_11935"
     CDS             1431..3419
                     /locus_tag="VFQ23_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylosucrase"
                     /protein_id="HET9907351.1"
                     /translation="MFNITNIERESIITLKRLLPRVEREFSAAMASDSAGWIEFSERL
                     QMQFPSLFQLYFALYKDRYDFFFHLEDLLFSLARAWLQRPDDLRKLDKEREKNPVWFQ
                     SNQMVGGVCYVDLFANDLEGIKSKIPYFEEIGLTYLHLMPLFKAPEEENDGGYAVSSY
                     REVHPPLGTMDQLASLARELRQAGISLVVDLVFNHTSDEHIWAERAKAGDAEYMDFYR
                     IFPDREIPDAYELNLREIFPDEHPGAFTFFPELFPSPISEGNEGRARDGGWVWTTFHT
                     YQWDLNYANPAVFNRMAEEMLFLANQGVEVIRLDAVAFIWKELGTSCENLPGAHTLIR
                     AFNTVARIASPALIFKSEAIVHPDDVVKYIAPEECQISYNPLLMALLWNALATRKVRL
                     LSQALATRFRIQPETAWVNYVRVHDDIGWTFSDEDAAILGINASDHRQFLNEFYRGRF
                     PGSFSRGLPFQENPKTGDCRISGTCASLAGLEKALKEEGPEQVELAIKRILLMHGVIL
                     TVGGIPLLYLGDEIGTLNDYGYQDDPAHQADSRWVHRVKADLDLYEKRSDPNTIQGRV
                     FQGLLKLIKLRKANPAFAGGDLEMVPTSNDHVLGFIRRTTDEQAIVFANFSEVTQTVP
                     SLVFENHKLEIKEKLHGDSNYVAGQEFAIEPLEFLVFN"
     gene            complement(3416..4870)
                     /gene="pyk"
                     /locus_tag="VFQ23_11940"
     CDS             complement(3416..4870)
                     /gene="pyk"
                     /locus_tag="VFQ23_11940"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET9907352.1"
                     /translation="MTRKAKIVATIGPASESAELLEELILSGVNVARMNFSHGTHEQH
                     AERIALIRQLEEKLGAPVGILQDLQGPKIRVGALTKIIQLAEGEIVVLHATGMPPPDE
                     SGTMIPVDFRQLFDSVRISDRLLLDDGRLTLEVVSVDRSHYALKAKVIVGGPLTSHKG
                     INLPGVRLRISGFTEKDEADLAFGIAQNVDAVAISFVRTAEDIKKVRYAMERLSNGKR
                     LPLLIAKLEKPEALDNLDAILDLVDGVMVARGDLGVELPPEKVPVFQKHIIHTANARA
                     KLVITATQMLESMIMNPLPTRAEASDVANAVFDGTDAVMLSAETAAGKYPVESVQMMD
                     RIVREAETHFLEWGTEQHVYDFEQSDAASMARAAQALANDKNVTAVACFTTQGKTAWL
                     MSKIRPRVPIMAFTPSQDTYRRLAFMWGVRPQKIDFAKTLEEMIDHVDAALMRSDVVH
                     PGDQVVLVCGFPVGALRPPNMALLHDVGERITSP"
     gene            complement(4933..6135)
                     /gene="rocD"
                     /locus_tag="VFQ23_11945"
     CDS             complement(4933..6135)
                     /gene="rocD"
                     /locus_tag="VFQ23_11945"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HET9907353.1"
                     /translation="MNTQDYIALEEQYGAHNYHPLDVVIERAEGVWVYDVDGKKYLDC
                     LSAYSALNQGHVHPEILNALLGQAKKVTLTSRAFRNDQLPLLYQELSEMTGYEMSLPM
                     NSGAEAVETALKLARKWAYQVKGVPRHQAEIITAEGNFHGRTISIITFSSEPSYREDF
                     GPFTPGFKTVRYGDASAIEAAITPNTAAVLIEPIQGEGGVIIPPDGYLKQVAEICKKH
                     NVLLMADEIQTGLARTGKLFACEYEGVRPDVTIVGKAMAGGFYPVSAVLADAAILGLF
                     KPGEHGSTFGGNPLAAAVARASLRVIREEKLAERSQQLGEYFMEQLSEIPSPHVKEVR
                     GKGLLIGVELKESAGGARRFCEALQVNGILAKETHDNVIRFAPPLVIDKETIDWALPS
                     IREVLNMN"
     gene            complement(6190..7113)
                     /gene="argF"
                     /locus_tag="VFQ23_11950"
     CDS             complement(6190..7113)
                     /gene="argF"
                     /locus_tag="VFQ23_11950"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET9907354.1"
                     /translation="MKHFLAVSDLSSGEVQDLLDLAIRLKEEYFKQGNAPLFKGKVLG
                     MIFQKPSLRTRISFDMAMRHMGGDALYLSPNEIGLGKRESIADIARVLSGYLHALMAR
                     TFDHAHVLELAKWSEIPVVNGLSDYNHPCQGIGDALTIQEKFGTARGLNVAYVGDGNN
                     VAVSLMHVCAKLGWNFTIASPEEYDLSPKAVELADDIVKETGSKLTFLRDPHVAVRGA
                     HVIYTDTWTSMGQEEESAKREKVFPPYQVNAQLVSEADKDVIVMHCLPAHRNHELTDE
                     VADGPHSVIFPQAHNRLHAQKAILARLFGVA"
     gene            7298..7915
                     /locus_tag="VFQ23_11955"
     CDS             7298..7915
                     /locus_tag="VFQ23_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907355.1"
                     /translation="MSNNIPAGAWLVGGGSKDEMVDAFREKGAIALDWPDIGDLALIS
                     SLDEFKMRLLQTFSGNAPDHIREELKYLLWFMRLIAVDDYALIDKKQTDELIVGQVTS
                     AVEYNPELFGVRYPYIRRMKWLKHIPRDLFTLEAQQDLYSILPIEDIRSHRYEIHKHI
                     TGKDYSEKFGPYQDIGVLKAFVRELAGETDDFLQSIWNIYSGGTR"
     gene            7949..8305
                     /locus_tag="VFQ23_11960"
     CDS             7949..8305
                     /locus_tag="VFQ23_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907356.1"
                     /translation="MNWRKLFNKPKDLAAQYFLQYYLRRYITRMLELSIDNKTRKIHA
                     VVELKGEDQPIMIDVQYDLAIQKNTDEALVKANSITISKEWLPLIAQEVIGREFSIQG
                     KDSTNMIQLIKNLGIL"
BASE COUNT         2116 a         2113 c         2029 g         2067 t
ORIGIN      
        1 cgactactac aacgtggcgg agacgtttgg cacactggaa gatttcaaaa ccctgattca
       61 agaagcgcat aatcgaaata tccggctcat cgcggacctc gtgttgaatc acacatccga
      121 tgagcatccc tggttcaaag ccgcgcgtgc cgatcgcaac tcgccctacc gcgactatta
      181 cgtttggagc gacaccgatc aaaaatatca agacgcacga atcatctttc ttgacaccga
      241 gccatccaat tggacatggg atgaagtagc cggtcaatac ttctggcatc ggttctattc
      301 aacccagcca gacctgaact ttgacaatcc caaggtgcag gaggaaatga tcaacatcgc
      361 gcgcttctgg ctggacctgg gaattgatgg attccgcgcc gacgcggtcc cctatctttt
      421 cgaacgcgaa ggcaccaatg gcgagaattt gcctgaaacg catgtttact taaagaaact
      481 gcgcgccttt atggagcaga attatcccgg gcggatcctt ctgtgtgaag ccaatcaatg
      541 gcctgaggac gtgcggcctt actttgggga tggcgacgaa tttcacatcg ggtttcactt
      601 cccgatcatg cctcggatct ttatggcgct gaagaaggaa cgcgccgacg atatgattga
      661 cattttgaac cgtacacctg ccatccctga aaattgtcag tggtgcatct ttctgcgcaa
      721 tcatgatgag ttgacgctcg aaatggtcac agaagaggaa cggcaatgga tgtggcagca
      781 atacgcgccc gagccgcgta tgaaattgaa cctgggtatt cgccgccgac tggcaccctt
      841 actggataat gatcgtcgaa agatcgaact gattaattcg ttgttattta cgctccccgg
      901 ttcccccatt atttattatg gcgatgaaat tgggatgggc gataacatcg aattattcga
      961 tcgcaacggc gtccgcacac ccatgcaatg ggatgattca gaaaatgcag gtttcacaac
     1021 cggaaagccg ttcgctgagg tcattcgcgg cgatttcggt tttgaaaagg tcaacgtagc
     1081 cagccagctg gcagataaaa gctccgtgtt ctatgcaatc aagaaattga tccaaatcag
     1141 aaaacaatat cgcgcctttg gacgagggac gatggaatgg gtggaaactg gcaacccggc
     1201 cgtggcagcg tatctccgta agtacgaaag tgaaagcctg ctaatcctca gcaacttaag
     1261 tggctcccca caaaccataa accttccttc agaacatcac cgtgatgcaa cagatctctt
     1321 aaccgaaatt caggtcaggc tggaggcgac tctggaactt ccaccatatt cgtatcattg
     1381 gctaaaacaa taaacgaaga aacgtcacag gcgattgaag gtcctcactt atgttcaata
     1441 tcaccaacat cgagcgcgaa tcaataatca ctttgaagcg tttgttgccg cgcgttgaac
     1501 gggaattttc ggctgcgatg gcttcagact cagcagggtg gatagaattc tctgagcgct
     1561 tacaaatgca gtttccatca ctattccaac tctatttcgc tttgtacaag gatcgttatg
     1621 attttttctt ccacctggag gatttactgt tcagcctggc gcgtgcctgg ctccaacgac
     1681 ctgatgattt gaggaaactg gataaagagc gcgaaaagaa tccggtgtgg tttcaatcca
     1741 atcagatggt cggtggagta tgctacgttg atttgttcgc aaatgacctt gagggcatca
     1801 aatccaaaat tccgtatttc gaagaaatcg ggctcactta tcttcatctg atgccgctat
     1861 tcaaagcgcc agaggaagaa aacgacggag gctatgccgt cagcagttat cgtgaagtcc
     1921 atccacccct cgggacgatg gaccagcttg catcgcttgc gcgtgagttg aggcaagctg
     1981 gcattagcct tgtagttgat ctggtcttca atcacacctc cgatgaacac atctgggcag
     2041 aacgcgccaa ggcgggcgat gcagaatata tggatttcta tcgtatcttt ccagaccgtg
     2101 aaatacccga tgcctatgaa ttgaatctac gggaaatttt ccccgacgaa cacccgggag
     2161 cttttacttt ttttcctgaa ctgttccctt ctcctatttc cgaaggaaat gagggaaggg
     2221 ctagggatgg gggttgggtt tggacgactt tccacactta tcaatgggac ttgaactacg
     2281 ccaaccccgc ggtcttcaac cgcatggccg aagaaatgtt atttcttgcc aatcagggcg
     2341 tcgaagtgat acgcctggat gcggtcgcat ttatctggaa ggagcttgga acaagctgcg
     2401 aaaatcttcc tggcgcgcat acgctcatcc gcgctttcaa taccgtggcg cggatcgcat
     2461 cgccggcctt gatcttcaaa tcagaagcta tcgttcatcc ggatgacgtt gtcaaatata
     2521 ttgcaccaga ggaatgtcaa atttcctaca accccctctt gatggcactg ctttggaatg
     2581 cacttgccac gcgaaaggtg aggctgcttt cacaagccct cgccacgcgc ttcaggattc
     2641 agcccgaaac ggcctgggtc aattatgtgc gcgtccacga tgatatcggc tggacgttct
     2701 cggacgaaga tgcggcaatc ctgggaatca atgcttcaga tcaccgtcaa tttctcaacg
     2761 aattttatcg cggtaggttt ccgggaagtt tttcacgcgg gcttcctttt caggaaaatc
     2821 caaaaaccgg cgactgccgc atcagcggga cttgtgcttc cctggctggg ctggaaaagg
     2881 cactcaagga agaaggtcca gagcaagtgg agttggccat caagcgcatc ttactaatgc
     2941 acggagtgat cctgacggtg ggcgggattc cgcttttgta tctcggggac gagattggta
     3001 cattaaatga ttatggctac caggatgacc ccgctcatca ggcagacagc cgttgggtac
     3061 atcgtgtaaa agcagatttg gatctttatg aaaaaagatc agatccaaac accattcaag
     3121 gccgggtatt tcagggtttg ctcaaattga tcaagttgcg aaaagcaaat ccggcctttg
     3181 caggtggtga cctggagatg gtgccgacaa gtaatgatca cgttcttggg ttcattcgcc
     3241 gaaccacgga cgaacaagca attgtttttg cgaatttttc cgaagtcact caaaccgtcc
     3301 ccagccttgt gttcgagaat cacaaacttg aaatcaaaga aaaattacac ggggatagca
     3361 actatgtggc cggccaagaa tttgcaattg aaccccttga gtttttagta ttcaattagg
     3421 gtgatgtgat tcgttcgcca acatcgtgta agagagccat gtttggtggc cttaaagcac
     3481 cgacgggaaa tccacagacc agcacaacct gatctcccgg gtggacaaca tccgaacgca
     3541 taagggcggc atccacgtga tcgatcattt cttccagcgt tttggcaaaa tcgattttct
     3601 gaggtcgcac cccccacata aaggccaggc gccggtacgt gtcctggctg ggtgtaaacg
     3661 ccataatcgg cacacgtggc ctgatcttgg acatcagcca cgcagttttg ccctgtgtgg
     3721 taaagcaggc aactgcggtc acatttttat cgttggcaag ggcctgagca gcacgcgcca
     3781 tggatgctgc atcgctttgc tcaaagtcat agacatgttg ttcggttccc cattccagaa
     3841 agtgagtctc ggcttcgcgc acaattcgat ccatcatttg caccgactca accgggtatt
     3901 tgccggcggc tgtttccgcc gacagcatca cagcatccgt gccatcaaaa actgcgttcg
     3961 ccacgtcaga ggcttctgcg cgcgtcggca gcggattcat gatcatcgat tcgagcatct
     4021 gtgttgcggt gatgaccagc ttggcgcgcg catttgccgt gtggatgatg tgcttttgga
     4081 aaacggggac tttttcaggc ggaagctcca cgcccagatc tccgcgtgcc accatcacac
     4141 catccacaag gtcaaggatt gcatcgaggt tatctaacgc ttcaggcttt tcgagtttgg
     4201 caataaggag aggaaggcgc tttccatttg aaagcctttc catggcatag cggaccttct
     4261 taatatcttc ggcggtgcga acaaacgaga tagccactgc atctacattt tgagcgatgc
     4321 caaacgccag gtcagcttca tccttttctg taaaaccaga aatgcgcaat ctgactccgg
     4381 gaaggttgat tcctttatgg gaagttaagg gtcctcccac aatcactttc gccttcaacg
     4441 cataatgaga tcgatccacg gatacaactt ccagtgtaag acggccatcg tcaagaagca
     4501 gccggtcaga gattcgcacg gagtcgaaga gctgacgaaa atccactgga atcattgtgc
     4561 cactttcatc cggaggaggc atgccagttg catggagcac cacaatctca ccctcggcaa
     4621 gttgaataat ctttgtcaac gcgccaactc gaatcttggg tccctgcaaa tcctgcagaa
     4681 ttcccaccgg ggcaccgagt ttttcttcaa gctggcgaat caacgcgatt cgttcggcgt
     4741 gttgctcatg agtcccgtgt gaaaaattca tgcgcgccac gttcacaccg gagagaatca
     4801 actcttcaag tagttcagcg ctttcgctgg ccggaccaat ggtggcaaca atttttgctt
     4861 tacgcgtcat aatgaattcc tatcgcgccg agcagaggca tcgaacgcga taatgattat
     4921 acgccaccta tcttagttca tattcagcac ttcgcgaatg gaaggtaatg cccaatcaat
     4981 tgtttcttta tcaatgacca gaggcggtgc aaaacggatg acgttatcat gcgtctcttt
     5041 ggcaaggata ccgttgacct gcaaagcctc acagaatcgg cgcgcaccac ctgcagattc
     5101 cttcaactcc acaccgatca atagtccttt gcctcgcact tctttgacat gcgggctggg
     5161 aatctcagat aactgttcca taaaatactc gcctaattgt tgagagcgtt cggctaattt
     5221 ttcttcccga atcactcgta acgaggcgcg tgctaccgcg gctgccagcg gattcccacc
     5281 aaaggtagac ccgtgttcgc cgggtttgaa caaaccgaga atcgcagcgt cggcaagcac
     5341 agcagatacc ggataaaagc ctccggccat cgccttgccc acaatggtca catccgggcg
     5401 gacgccttcg tattcgcagg cgaacagttt tccggtccgc gcaagaccgg tttgaatctc
     5461 gtctgccatc agaagcacgt tatgcttctt gcatatttca gctacctgtt tcaggtatcc
     5521 atcaggcgga atgatcaccc cgccttcgcc ttgaattggc tcgatgagca cagccgctgt
     5581 gttaggcgtg atggctgctt caatcgcgct cgcatcccca tagcgcacgg tcttaaagcc
     5641 cggcgtgaaa ggaccgaagt cttcgcgata ggaaggctca gaagaaaacg taatgatcga
     5701 gattgtgcgc ccgtggaagt tgccctctgc tgtgataatc tctgcctgat gacgcggcac
     5761 gcctttgact tggtaggccc atttacgcgc aagcttgagc gcggtttcca ctgcctctgc
     5821 cccgctattc atcggcaggg acatttcata accggtcatt tcggaaagtt cttgatagag
     5881 cagtggcaat tggtcgtttc ggaacgcgcg tgaggtcagc gtgacctttt ttgcctgtcc
     5941 gagcaaggca ttcaaaattt caggatgcac atgtccctgg ttgagggcgg aatacgccga
     6001 aagacaatcc agatatttct tcccatctac atcgtagacc caaacgccct cggcacgctc
     6061 gatcaccaca tccagcggat gataattatg tgcaccatat tgttcttcca aagcaatgta
     6121 atcttgtgta ttcatttttt ccttattcaa tcatgtaggg acaggcagct gcctgtccct
     6181 acgtatattt tatgccacac caaacaaccg cgccagaatc gctttctgag catggaggcg
     6241 gttatgcgcc tgcgggaaga tcaccgaatg cgggccgtcg gctacctcat cggtcaactc
     6301 atggttgcga tgggcaggca ggcagtgcat cacgatcacg tccttatcgg cttcgctgac
     6361 taactgcgcg ttgacctggt aaggcggaaa caccttttcg cgtttggcag attcttcttc
     6421 ctgccccatg gatgtccatg tatcggtata aatcacatgc gcgcctctga cagctacgtg
     6481 cgggtcccgt aaaaaggtca atttgcttcc cgtttctttg acgatatcgt ctgcgagctc
     6541 aaccgctttt ggactgagat catattcctc cgggctcgca atcgtaaaat tccagcccag
     6601 tttcgcgcac acatgcatga gcgaaaccgc cacattattt ccatcaccta cataagcgac
     6661 attcaagccc ctggcggttc cgaatttctc ctggatagta agtgcatcac cgattccctg
     6721 gcacggatgg ttgtaatcgc tcagtccatt gacaaccgga atctctgacc atttcgccaa
     6781 ttccagcacg tgcgcatgat caaacgtgcg ggccatcagc gcatgcagat agcctgaaag
     6841 cacacgcgcg atatcggcaa tcgattcgcg tttgcccaag ccgatctcat tcggcgaaag
     6901 atacaacgca tctccgccca tatggcgcat cgccatatca aacgagatgc gcgtccgcag
     6961 gctgggcttc tggaagatca tgcccaatac tttccctttg aacaagggcg cattgccttg
     7021 cttgaaatat tcttccttca aacgaattgc caggtcgagc aaatcctgca cctcccctga
     7081 agataaatcc gagaccgcga gaaaatgctt catgtaagct ccttggtgaa ttttgccctg
     7141 tgagcagcat attattttgg acaattatac agaattaatt ctggtcagtt ctgaatttgt
     7201 aacttctcct ttctacggct gatagacagc aagatatacg cgtataattc aatggtatta
     7261 actccaagga cgagttggat ggagacttgt ttaactgatg agcaataata ttccggctgg
     7321 tgcgtggctg gtaggcgggg gtagtaagga tgaaatggtg gatgcattcc gggagaaagg
     7381 cgccatcgcg ctggattggc cagatatcgg cgacctcgca ttgatatcat ccctggatga
     7441 attcaaaatg cgcctgcttc agacgttttc tggcaatgcg ccagatcata ttcgggagga
     7501 gttaaagtat ctattgtggt tcatgcggct gattgccgtg gatgattatg cgctcatcga
     7561 taagaaacaa acggacgaat tgatcgttgg acaggttact tctgctgtgg aatacaatcc
     7621 tgaattgttt ggagtcaggt atccgtacat tcgacggatg aagtggctca aacatatccc
     7681 ccgagatctg tttacgctag aggctcaaca ggacttatat agtattctac cgattgaaga
     7741 tatccgatct catcgatatg aaatccataa acatatcaca ggcaaagatt atagtgagaa
     7801 gtttggaccc tatcaggaca ttggtgtcct taaggcgttt gtgcgtgaac tggccggaga
     7861 aactgatgat tttttacaat ccatctggaa tatctactcc ggaggaactc gataagcagc
     7921 tcggcacgag tggagtacga ttatctctat gaactggcga aagcttttca acaaacccaa
     7981 ggacctggct gcccaatact ttcttcaata ttacttgagg agatatataa cgcgtatgct
     8041 cgaactatcc attgataata aaaccagaaa aatccatgca gtggtggaac tcaaagggga
     8101 ggatcagccc atcatgatcg acgttcaata tgacctcgct attcaaaaaa atacggatga
     8161 ggcgctcgtc aaagccaaca gcatcaccat ctcgaaggaa tggctccctc tcattgcgca
     8221 ggaagtaatt ggccgggaat tttccattca gggaaaagac tcgaccaaca tgattcaatt
     8281 gataaaaaat ttggggatac tataagatag cgataagatc ctgaa
//
LOCUS       DASTJP010000414         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1377258, whole genome shotgun sequence.
ACCESSION   DASTJP010000414 DASTJP010000000
VERSION     DASTJP010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377258"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFQ23_11965"
     CDS             <1..286
                     /locus_tag="VFQ23_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907357.1"
                     /translation="VEDAIGEIARVLKPSGELIFCELGLAPDPSVQSWQRLLHPIYHW
                     LFQGLHLTRNMPDLIKQGGFRIRQLEAGYLASFPKSPSYYWSGIAQLKEQ"
     gene            complement(273..860)
                     /locus_tag="VFQ23_11970"
     CDS             complement(273..860)
                     /locus_tag="VFQ23_11970"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9907358.1"
                     /translation="MHTKVKTDPRVRRTRRILQEALVSLVLEKEYSAISIKDITHRAE
                     VAYITFFRHYDGIEELLMEVLDEGLAELLRHIATLAGEADGPANELEGKLIFEYVKQK
                     SDLFRILLKSQSVKRIRKSVIENIAAIFQNSCGFLQRPNSLMPANMASNHMATSLLAL
                     IEWWLEHKMSPPPLQMGKIYKALIIDSTRDTVIAL"
     gene            1014..2090
                     /locus_tag="VFQ23_11975"
     CDS             1014..2090
                     /locus_tag="VFQ23_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907359.1"
                     /translation="MKTLVLNRLQSKTGLPDYASVSLRFEWLFILASLWLFTGLYLDG
                     WAHNNIPEQIDSFFTPWHMVLYSGYGVAAAILGAQYLLNIRKGYSWLKALPSAYMTSL
                     LGAIIFATAGNLDFIWHAVFGFEEDVEALVSPSHLSLAVGGVMIMSGPLRAAWRQSHS
                     KEETITSRALLSLFILIGVFTFFTQFSNAFAHPQVFVGRSPLGDTYFWDVALISYVLI
                     PTLILMAAIIMVMLRWRLPLGSLTLLITGNSVLMFLMAWRYSHAQWPVLIAALFGGFL
                     ADVLLLGLKPSTQRVKALRWFSFLTPALLFLAYFVALFLTDQVWWNSNMWLGMIFFSG
                     ILGLGLSWLAVPPYESDTNLSVEN"
     gene            2398..>2988
                     /locus_tag="VFQ23_11980"
     CDS             2398..>2988
                     /locus_tag="VFQ23_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907360.1"
                     /translation="MYKLKFLSLVLFLVLLSSCAPILATASPAPASTMAPPTVSAAVA
                     QSVEVQILDGEPLQVNAIIRGQLPDGGCTTISSVDQVRNGNTFNIQLMTTTDPLAICT
                     LTATPFEQILPLQVSNLPPATYTVNANGVEATFELLPRDPSAFQQVLVDALNRRDYEL
                     QKILMGRSFMIGYWLSEGTENTPDDAIQQLQTNLLSP"
BASE COUNT          703 a          741 c          696 g          848 t
ORIGIN      
        1 cgttgaggac gctattggag aaatcgcccg tgttctcaaa ccaagcggcg agctgatttt
       61 ctgtgaactt ggtcttgccc cggacccatc ggttcaatcc tggcaacgac ttttgcatcc
      121 gatttaccat tggctgtttc aggggctaca tcttacaaga aatatgccag accttattaa
      181 gcaaggtggt tttcgtatca ggcagctgga agcaggatat ttggcatcat ttcccaaatc
      241 accatcctac tactggagcg gtattgcaca gcttaaagag caatgacagt atctctcgtt
      301 gagtcgatga ttagagcttt atagatcttt cccatttgca gaggaggggg gctcatcttg
      361 tgttccaacc accattcgat aagcgcgagc agagaggtag ccatgtggtt tgacgccatg
      421 ttggccggca taaggctgtt cggtcgctgg agaaacccac aggagttctg aaatatcgcc
      481 gctatatttt cgattacaga cttgcggatg cgcttcacgc tctgactttt gagcaggatc
      541 cggaagagat cggatttttg tttaacgtat tcaaaaatga gtttgccctc caactcattt
      601 gccggaccgt cggcttcacc agccagtgtg gcgatgtgac gcagtaattc ggccaagccc
      661 tcatccagaa cctccatcag caattcctca attccatcat agtgcctgaa aaaggtgatg
      721 taagcaacct cggcacggtg cgtgatgtct ttgatggaaa ttgccgagta ctccttttcc
      781 aagactaacg aaaccaatgc ctcctgcaaa atacggcggg tgcgccgaac tcttggatct
      841 gttttcactt tggtatgcat ctttatgtcc taaacctggg gagctacttt tgttacaaaa
      901 cagagatctt agttacttat gttatagcga gccaccattg attattgaca tggaggcaac
      961 aatagatata ctcaaacaca tatgttatag agtataacac aaggagaaat caaatgaaaa
     1021 ctttagtatt aaaccgatta cagtcaaaaa cagggttacc ggactatgcc agtgtaagtc
     1081 tccgttttga atggctgttc attttggcat cactttggct ttttaccgga ctttacctgg
     1141 atgggtgggc acacaacaac atccctgagc aaatcgatag cttctttacg ccctggcaca
     1201 tggtcttgta cagcggctat ggagtggccg cagcaatttt gggtgcacag taccttctca
     1261 atataaggaa gggctatagt tggttgaaag ctctcccgtc cgcgtatatg acctcgttgc
     1321 ttggagcgat catcttcgcc accgccggta atttagactt catctggcat gcagtatttg
     1381 gttttgaaga agacgtagaa gcattggtca gcccctcgca tctcagtctg gctgtcggtg
     1441 gagttatgat aatgagtggt ccactgcgtg cagcttggcg gcaatcgcat tcaaaggaag
     1501 aaacaataac ctcgcgcgca ttgctttcat tgttcattct catcggtgtt tttacgtttt
     1561 ttacccagtt ctctaatgcc tttgcccacc ctcaggtgtt tgtagggcgg tcccctctgg
     1621 gagacaccta tttctgggat gtagcactga tttcctatgt attaattcca accctgattc
     1681 tcatggctgc cattatcatg gtcatgctac gctggagatt accattaggc agcctcacgc
     1741 tcctcattac gggaaattct gttcttatgt tcctcatggc atggcggtat tcgcacgccc
     1801 aatggccggt tttgattgcc gcgctattcg ggggcttcct ggctgatgtg ctgttgctcg
     1861 gccttaagcc atctacccaa agagttaaag ccttacgttg gttcagtttt ttgacgcccg
     1921 cgttgctgtt cctcgcctat tttgtggctt tgttcctaac ggatcaggta tggtggaact
     1981 caaacatgtg gctcggcatg attttcttta gcggcatttt aggtcttggc ctgagctggc
     2041 tggctgttcc cccctacgaa tcggatacaa acttaagcgt ggagaactga tcagagcttc
     2101 aacgtacttc aaaactgttt tgcatgcaat ttctgttgag gtcgcagctt cattgcgacc
     2161 tttttttgtt taaatacagg aactccgtcc aggtcattcc cggttggtcg ctggccgatc
     2221 aatccttgcc gaaggaggcc aagcactgcc cggactcaca ttggaaatca agatcgtgcc
     2281 gaacgctttg tgtcactttt tgtccatgtt cttgtagagt ttttccatac caacaaagga
     2341 accttttttt gtataaagtc atcatcactc aactcaagag agagaaggag actttatatg
     2401 tacaagctta aattcctatc ccttgttttg ttccttgttc ttttatcgag ctgtgcaccg
     2461 atcctggcaa cagcttcccc agctcctgct tccaccatgg cacctccaac tgtaagtgca
     2521 gccgttgccc agagcgtgga agtccaaatc ttggacggcg agcccttgca ggtcaacgcc
     2581 atcatccgcg gacaactgcc cgatgggggt tgtacaacta tttcgtctgt cgatcaggtg
     2641 cgtaacggga ataccttcaa tatccagctg atgaccacta cagacccgct cgcgatctgc
     2701 accctgaccg ctacaccctt cgaacagatt ctaccgttac aggtgagtaa cctgcctccc
     2761 gccacatata cagtgaatgc gaacggtgtg gaagctacat ttgaattact accgcgcgat
     2821 ccctcggcgt tccagcaggt ccttgtggat gcgctgaatc ggcgtgatta cgaattgcaa
     2881 aaaatcctga tgggtagatc cttcatgatc ggttattggc tctccgaagg aacggagaac
     2941 acgccggatg atgcgatcca gcaattacag acaaatttac tcagcccc
//
LOCUS       DASTJP010000415        12681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_998318, whole genome shotgun sequence.
ACCESSION   DASTJP010000415 DASTJP010000000
VERSION     DASTJP010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12681
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998318"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..876)
                     /locus_tag="VFQ23_11985"
     CDS             complement(223..876)
                     /locus_tag="VFQ23_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9907361.1"
                     /translation="MLRFRYLGDYYFAHRRGWLDIFADTSEIYAKIHRKHGLPTIVAH
                     WGATPQWYKNLDLERDIDVLWMGTRGTKRRSLILDRVRSKLESSGVHMHIADGKENPF
                     IFGEERIHYLNRSKITLNITRTWYDDNFSRIALAAPNRSLVVSESILPHCPSLITGIH
                     FVSSPIEQLAETILYYLAHERERLRIIDNAYQFVTTSLLFRESVRSIMDAVNHLYQS"
     gene            complement(1339..2916)
                     /locus_tag="VFQ23_11990"
     CDS             complement(1339..2916)
                     /locus_tag="VFQ23_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9907362.1"
                     /translation="MVTPKALFELLVNSAKIDPDKTAIIEPGQGSITYGEFDALSDQL
                     SDRLYHLGVRQGDRVGVYLHKSIDSLVTIMGILKIGAAYVPVDPGAPPSRNAYIFADC
                     QVKALLIEDRFVNDIKAEWGASGHTSQLLILNQTGGGKYLKEMLDQAQTLEPVSPRAG
                     YVASLNDLAYILYTSGSTGKPKGVMLTNRAAISFVDWCSEAFEPHSDDRFSSHAPFHF
                     DLSILDIYVPVKHGASLVLISEEIGKDPIRLASVIAESKITIWYSAPSILGFLAQYGK
                     LEQYDYSNLRTVLFAGEVFPVKHLRALKKLLPNPRYFNLYGPTETNVCTFYEIPLFVP
                     DDRTQPYPIGIACSNYRIRVLDELGNEVLPGHEGELCASGSGMMIGYWNLPERTANAF
                     LVDSAGDKWYKTGDIVIEGADGNYIYVARRDRMVKKRGYRIELGEIEAGLYRHPAIKE
                     AAVVATQNDEGVKIRAFVSHHKDNRPSIIELKQFCAQNLPAYMVPDLFSFLDFLPKTS
                     TDKIDYQKLKEFSSTVV"
     gene            complement(3111..3293)
                     /locus_tag="VFQ23_11995"
     CDS             complement(3111..3293)
                     /locus_tag="VFQ23_11995"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HET9907363.1"
                     /translation="MKNFIRSMGSNFGVVGELLSFLWQRKLWWLIPMVVVLLVFGLLL
                     VFASASGIGPFIYTLF"
     gene            complement(3378..5168)
                     /locus_tag="VFQ23_12000"
     CDS             complement(3378..5168)
                     /locus_tag="VFQ23_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HET9907364.1"
                     /translation="MYILGISCYYHDSAAALLHDGILVAAAEEERFTRVKHDYSFPSR
                     SIDFCLRQAGITANDLDYVVFYEKPLQKFERILMSTLQTFPQSWGVFRESMITWFNDK
                     LWVKGHILTTLNIPDEKLLFVEHHLSHAASAVFCSPYDEAAVLTIDGVGEWTTATIGK
                     ATADWDGNGKNQIDLFGEVKFPHSLGLLYSAFTAFLGFQVNEGEYKVMGMAPYGKPTR
                     VEDVYKLFEVGDDASFRLNMEYFSFHHSTSRTFNGNFTKLFGDPRVHESDFYTPTTHP
                     KKDHPQWDESTAKLNQHYADIAASIQYATEETMLKMANYAHQRTGLKKLCMAGGVALN
                     SVANGRILRETPFEDVYIQPAAGDSGGALGAALYVYHVLLGKPRKFVMEHAYWGQAYT
                     PEETKRDIEATGRSYELVEDPNRRAEMMVDDMLAGKVIGLFQDRFEWGPRALGNRSIL
                     ADPRSAEMKNIVNARIKFREPFRPFAPVILEERASEFYSGTGDPTRNFPLRYMLMVCP
                     TRDGCGDQIQAVTHEGGTGRVQSVQRESNPLYYRAIELFGQATGVPVLLNTSFNLRGE
                     PIVTTPANALNTFGNSDIDTLYVNDFVVRK"
     gene            complement(5168..5398)
                     /locus_tag="VFQ23_12005"
     CDS             complement(5168..5398)
                     /locus_tag="VFQ23_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907365.1"
                     /translation="MEALQKFWHAWKRFGQFMGDLIGRLVLTVFYFTLFMPFGLGVRF
                     FGDPMALHPKGPSKWLQRTTKDLTIDDTRRLY"
     gene            complement(6327..7358)
                     /locus_tag="VFQ23_12010"
     CDS             complement(6327..7358)
                     /locus_tag="VFQ23_12010"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET9907366.1"
                     /translation="METSSHRRLNPLTGEWVLVSPQRANRPWHGQVESVPLESLPEYD
                     PDCYLCPRNQRVGGTRNPDYSSTFIFDNDFSSLLPAENFVQTVDHSLLTSSNESGICR
                     VVCFTPRHDLTLPELALGEIENIINTWKQQTLELSAKDFVKYVQIFENKGAMMGCSNP
                     HPHCQIWGTQYLSNEPAKELEYQTTYLSQHGRPLLADYLHEEQVRNKRILFSNDHFTV
                     LVPYWAAWPFETMIIAHRKVAWLSELTLEEIGALAETLQRITSRYDNLFESSFPYAMG
                     FHQAPFDGRSHPEWILHAHFYPPLLRSATVRKFMAGYELLVMPQRDITAEAAAERLRS
                     ISDLHYKLH"
     gene            7935..8423
                     /locus_tag="VFQ23_12015"
     CDS             7935..8423
                     /locus_tag="VFQ23_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HET9907367.1"
                     /translation="MQSKPRKEVLLRERLRIQRIEAYLPSIRVNPVNPRARKEQPFFP
                     GYLFIHADLEKFPISEFRRIPGSIGLVCYGGEPAHIGDALIREIQKQVEEISDLTTNY
                     VDRFRPGDLVVINDGPFASYKAMFDCRLSGSDRVRVLLELLQDQKMRVELSGKQLQLL
                     TS"
     gene            8590..10098
                     /locus_tag="VFQ23_12020"
     CDS             8590..10098
                     /locus_tag="VFQ23_12020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9907368.1"
                     /translation="MSIQTQIEHSSIIAASKKIKAASSIIAPHLQWKLYITGLMTSDL
                     LVTFAAFWLSYNSRFEWFDQYFVPTEGVPFATYRFLLYTVPFLWLVIFAINGLYIRSN
                     LLGGTREYSKVFSSATTGFLLIIIVSFLDPTLIIARGWLLLTWGSTFLLVTLARLLLR
                     RIVYRLRRHGFFVTPAVIVGANEEGRSLAEQLRRWKSSGLHLVGFVDKKTPATIPVFH
                     QLACLGSVDQLGEIISRYQIGEVILASSAFSTRDYLLDIFRAYGLTDRVNIRMSSGLY
                     EILTTSLTVSEYAYVPLVYVNKVRLTGSDAFLKLIFDYILTLAGLIILLPFILLIAIW
                     IKISSPGPVFHKRLVMGLNGKRFYALKFRTMAINGNEILEQYPELKDELARNHKLKTD
                     PRITQIGAFLRKYSLDELPQLVNVVKREMSLVGPRMISPEEVSMYKQFDMNLLTVLPG
                     ITGLWQVSGRSDISYEERVRLDMYYVRNWSLWLDLQLIFQTLPAVLRAKGAY"
     gene            10150..11130
                     /locus_tag="VFQ23_12025"
     CDS             10150..11130
                     /locus_tag="VFQ23_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HET9907369.1"
                     /translation="MIIVQTPLRVSFFGGGTDFRAFFMQEGGCVLSSAIDKYIFITIK
                     KRFDNKLRIGYTQTEMVDEVDQIHHELIRESLRITRISHGVEITTMGDIPSEGSGLGS
                     SSTVTVGALHAMYAYLGEMVPAERLAHEACEVEIEILKRPIGIQDQYIAAYGGLRFFE
                     FLPDGRVTAESIKLSPEAQRALNNNFMLFFTGISRTSSSILKEQVNNLKDRLSELREI
                     KNMAREARFAIESENFDTLGVLLHQSWELKKRLAGTISNGQINEMYEASRRAGAIGGK
                     VAGAGGGGFLLLHVPIERQDKVRAALHGLQELPFRLEADGTKVIFNYRRL"
     gene            11153..11767
                     /locus_tag="VFQ23_12030"
     CDS             11153..11767
                     /locus_tag="VFQ23_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9907370.1"
                     /translation="MEYIQEYITTLQLTMDQLPRQLIADVIDVLQQARRKGSQVFILG
                     NGGSASTASHFACDLAKNTRQTGLPHFRVIGLTDNMAMFSALANDEGYENVFSEQLAG
                     LARPGDIVIAISASGNSKNVIYAAEAAHRYEATVIGFTGFDGGRLAQLADINIHVNSN
                     VIEHVEDIHLMLEHMIVKTIKEQVQTSVIMTTSLAQIPAENFGD"
     gene            complement(11764..12342)
                     /locus_tag="VFQ23_12035"
     CDS             complement(11764..12342)
                     /locus_tag="VFQ23_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9907371.1"
                     /translation="MYPSIFLDRDGVLIENRSDYVRDWSQVKIIPEAIRALSLAPMKK
                     YKVVIVTNQSAVGRGLLLLKTAQEINQRLIELIHDHGGQIDSVYMCPHRPEDGCSCRK
                     PLPGLLLQAAHDLSLDLRRSWMIGDAWTDIQAGEAAGTRGTILLKTGRGINQLLQGQP
                     DKISENLVFDNLALALKAIFSIDDDPAIRSHS"
     gene            12445..>12681
                     /locus_tag="VFQ23_12040"
     CDS             12445..>12681
                     /locus_tag="VFQ23_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907372.1"
                     /translation="MPTATMLKYIRLYLDRQASSLLRYFYEQAFLVSLSWIPTVVGIG
                     LRGVFYPFILQMEGLAAIENNVRLRFADHIRLGHG"
BASE COUNT         3510 a         2738 c         2893 g         3540 t
ORIGIN      
        1 tgccttcatt cttgggctcc ggctagctta gactcaatta agccttgcct ttgacgcaga
       61 ttcaaattag ttccctaaag caagttgagg atatcagaca aggatggctt tgaatcgtgc
      121 tagtgtaagt aaatcttacc aagtgagcta tgttgctcgc ccaaagttca aaatccattt
      181 ttttgtggtc acctggataa ttcatgagaa tttagaatag agctaggatt gatagaggtg
      241 atttacggca tccataatgg acctaacact ttctcgaaac agcagtgaag ttgtaacgaa
      301 ctgataagca ttgtcgataa tccgtaatct ctctctttca tgcgccaaat agtataaaat
      361 tgtctcagca agttgttcga ttggagatga cacgaagtgt attcctgtaa tcagacttgg
      421 acaatgaggg agaatgcttt cggaaacaac caatgaccgg tttggagctg ctaaagcaat
      481 tcgggaaaag ttgtcatcgt accaggtgcg ggtaatatta agtgtgatct tggatcgatt
      541 tagataatga atccgctcct cgccaaagat gaatggattc tctttgccgt cagcgatatg
      601 catgtgaact ccgctcgact caagtttgct tcgcacccga tccaaaatca aactccgtct
      661 ttttgtgccg cgggttccca tccacaaaac gtcaatatct cgttccaaat cgagattttt
      721 gtaccattgg ggcgtggctc cccaatgggc cacaatggtt ggtaacccat gtttgcggtg
      781 tatctttgca tagatttccg atgtatcagc gaagatatct aaccaacctc gacgatgtgc
      841 aaaatagtaa tctccaagat aacgaaaacg caacattcgg gtctcgatta aagagagaag
      901 cctttctgtt ccgggtattg ttgcagaact attccgagaa tcccgaactc ttcctaacca
      961 tgaacgccat ctggagatcg aactcatgat tttccagggg attctcagat caggaattcc
     1021 ttcagtgttc caatggacga aaattggctt ctgagaagag ggaattttcg ataattgact
     1081 cgggatagtt aagaactttc cgtatggacc aaaagagaaa actacctcgg ctttttgagg
     1141 tatgacagag ccaatttgaa aatggatggg ctggtaaccc aaagcattta gttcatcggc
     1201 gattacttca ttgataccgt ctatatcgtt ttggtgtgga atcgcaactg tcactggcct
     1261 atccacgctc ggtattaggg gattcatttt tggtccaacc gcatcgaagt ccattttagt
     1321 tatctcctcc caaagatgct acacaacagt tgaggagaac tcctttagtt tctggtaatc
     1381 gattttatct gtggatgtct ttggaagaaa atccaggaaa gaaaagaggt caggcaccat
     1441 gtaagctggc agattttgcg cgcaaaattg ttttaattca atgatcgaag gacggttatc
     1501 cttgtgatgg ctaacgaagg ctcttatttt taccccttcg tcattttgcg tggctaccac
     1561 ggcagcttct ttaattgccg gatgtctgta taaacctgct tcgatttcac ctaactcaat
     1621 acgatatccc cgctttttga ccatgcgatc tcgccgggct acataaatgt aatttccatc
     1681 tgccccctca attacgatat cgcctgtctt ataccattta tcccctgcgg aatcaacgag
     1741 aaatgcattt gctgttcttt caggcaggtt ccagtaaccg atcatcatcc ccgaaccgct
     1801 ggcgcataac tcaccctcat ggccgggcaa cacttcgttg ccaagctcat caaggactct
     1861 aattcggtag ttggaacacg ctattccgat agggtagggt tgtgttcggt cgtccggaac
     1921 gaacagcgga atttcataga atgtacaaac gttggtttct gtcggaccgt aaagattgaa
     1981 atacctggga tttggtagca gtttcttcaa agcgcgaaga tgcttcactg gaaagacctc
     2041 cccagcgaac aggaccgtcc gaagattgga ataatcgtat tgttccaatt tcccgtactg
     2101 ggccaggaaa cccaggatgg agggtgccga ataccaaata gtaatcttgc tttcggcaat
     2161 cacactagcc aaacggatag gatctttccc aatttcctcg gagatcaaaa ctagtgacgc
     2221 accgtgttta actggcacat atatgtccaa gatcgaaaga tcaaaatgaa aaggcgcgtg
     2281 agaagagaaa cggtcgtcag aatgaggttc aaaagcctcc gagcaccaat ctacgaagct
     2341 gattgctgct cgattggtga gcatgacccc tttgggctta ccagtagaac cagatgtgta
     2401 aagtatataa gcaagatcgt taagagaagc aacatagcca gctcttggtg ataccggctc
     2461 cagcgtttga gcttgatcta acatctcttt aagatatttg ccgccgcctg tctggttcaa
     2521 tattaataat tgagaagtat gcccactcgc accccactcg gccttaatgt cattgacgaa
     2581 gcggtcttca atgagtagag cctttacctg gcagtcagca aaaatataag cattcctgga
     2641 aggaggggca ccgggatcca ctggcacgta agcagcgcct atcttcaata tccccataat
     2701 cgttaccaag gaatcaatgg atttatgcag ataaaccccg acacgatctc cttggcgaac
     2761 acccaggtga tatagtcgat cacttagttg atcagaaagc gcgtcaaatt caccatacgt
     2821 gattgagcct tggcccggtt caatgatggc ggttttatcg ggatcgatct tggcagaatt
     2881 tacaagaagc tcaaacaaag cctttggggt taccacagtg ctacttcctt atttaaacca
     2941 agatgacgat ggcaatagag acgattcgcg cttagacgat gcctactctt cccaaggtca
     3001 attgtttatg gactcgcagt ccgatcaggc aagctccatc ggctaggtat ttgtggtagc
     3061 tttcgcattc tatcgcggct gaacaaatgc ctgaccggac tgcaataaaa ttagaaaaga
     3121 gtgtagataa atggtccgat gccggaagcg gaagcaaaga caagtagtaa tccgaatacc
     3181 agcagaacaa ctaccatggg gatcagccac caaagcttgc gttgccatag aaatgacaat
     3241 aactcaccta ccactccaaa gttggaaccc atgctgcgaa tgaaattctt cattgtaacc
     3301 attcctttct tttgtctgtg tttttattcc aataacaagt aatcagatta tttactggaa
     3361 acgctctcaa ctaaagttta tttcctgacc acaaagtcgt ttacataaag ggtatcaata
     3421 tcgctattcc caaaggtgtt caacgcattt gcaggtgtgg ttacgattgg ttcaccacgc
     3481 aagttgaagc tcgtattcaa taggacaggt acgccagtag cctgcccaaa caactcgatc
     3541 gctcggtaat ataaggggtt actttcgcgt tggacactct gtacacgtcc tgtaccgcct
     3601 tcatgtgtta cggcctggat ttgatcgcca catccatccc tggtcgggca gaccatcagc
     3661 atatatctca acggaaaatt acgggtgggg tcgcctgtgc cagaataaaa ttcactagcg
     3721 cgttcttcaa gaatcactgg cgcgaagggc cggaagggct cgcggaattt aatgcgggca
     3781 ttgacgatat tcttcatctc agcgctgcga ggatcagcca aaatagagcg atttcctagc
     3841 gcccgaggtc cccactcaaa gcgatcctgg aaaaggccaa ttaccttacc tgcgagcatg
     3901 tcgtctacca tcatttccgc gcggcgattg ggatcttcga cgagttcgta agaacggccg
     3961 gtagcttcaa tatccctctt cgtttcttca ggagtatagg cttggcccca gtaggcgtgc
     4021 tccatgacga atttcctggg tttgcccaac agcacgtgat agacatagag agccgctccg
     4081 agagcaccgc ccgaatcgcc tgccgcgggc tggatataaa catcctcgaa tggtgtctca
     4141 cgtagaattc ggccattggc gacggagttc aaagcgacgc cccccgccat acataatttc
     4201 ttgagaccgg tacgctgatg agcgtaattc gccattttca acatggtctc ttcagtagca
     4261 tattgaatac tggcggcgat gtctgcgtag tgctgattta gcttcgcggt ggattcatcc
     4321 cattgtgggt gatccttctt agggtgggta gtgggtgtgt agaaatctga ctcatgcacc
     4381 cgtggatcac caaataactt cgtgaaattt ccattgaagg tcctggacgt ggaatgatgg
     4441 aaagagaaat actccatgtt caggcgaaag ctagcgtcat caccgacctc gaaaagtttg
     4501 tagacatcct caacacgagt gggttttcca taaggtgcca tacccattac tttatactca
     4561 ccttcgttga cctggaaacc caaaaatgct gtaaacgctg aatataagag tccaagagaa
     4621 tgagggaatt tcacctctcc aaataggtcg atctgattct taccattgcc atcccaatcg
     4681 gccgtagctt ttcctatagt tgcggtagtc cactccccaa cgccatcgat cgtcagaaca
     4741 gcagcctcat cgtaaggaga acagaaaaca gcgctggcag cgtgacttaa atggtgctcg
     4801 acgaaaagca gtttttcatc tggaatattc agagtggtga ggatatggcc tttgacccac
     4861 agtttgtcat taaaccaggt gatcattgac tcccgaaaaa ccccccagga ttgagggaaa
     4921 gtctgcagcg tactcatcaa gatccgctca aacttttgaa gcggtttctc atagaagact
     4981 acgtaatcca ggtcgttggc tgtaataccg gcctggcgca ggcaaaaatc aatggaacgg
     5041 cttggaaagc tgtagtcatg ctttacgcgc gtaaagcgct cttcctcggc ggcagccacc
     5101 aatataccgt cgtgcagtag agccgcggct gagtcgtgat aataacaaga tatacccaag
     5161 atgtacatta gtagagcctc cgtgtatcgt cgatagtcag atctttagtt gtgcgttgca
     5221 gccattttga agggcccttt ggatgaagcg ccattgggtc accgaaaaaa cgcaccccca
     5281 aaccaaacgg catgaacaag gtaaagtaga aaacagtcag gaccaggcgg ccgataagat
     5341 cgcccatgaa ttggccaaac cgcttccagg cgtgccagaa tttttgtaat gcttccattt
     5401 tcctaccact ccatatgaaa aaaattatcc tccagtaatt ccctacgaat gaaaattctt
     5461 caccgggctt tactaaaggt gttgcacgat aaacttccga aattggactt gcttagagag
     5521 aacagatatg ttgcgggctt caccaggttt gtttattatg aaaatccccg caacttgact
     5581 actgtataca tggctaagat gtctcttgta gtgcttatac tggagctagc tcttttagta
     5641 aatcaggaaa tgagcgggga attcctctcc ctgggattac ttttcaaaaa ctcaaatcgc
     5701 ttactttgag tataaaacta gttcttcaaa ttttttattg acgcgctccg cccttccgac
     5761 aagagaaatt cgagatttgc atatttactt gtttgaaaag caataaatct gattcaattg
     5821 tcgaaacgaa gcagctggca aactacttcc acagtgttgc acttgagacc atgacgggta
     5881 ttttcgaaaa aggttgcaag tgtcgtggaa aaacttttca tgcgcccacg cgtgacaagt
     5941 cggtatgctg ggttttaaac ttaatagatt cttaagaggt aaagcttttg aaaaatggac
     6001 caacttcaat aatgtcatga actcaggatt tcggtcttaa gacaaaaaga tcatatcagg
     6061 tttatttaac cgggtcaata aatcataaag caattgtaat gtgatcgatt taaaatgagg
     6121 catgcaaaac tttcgttttc tgtttctacc atctatccca attgaaaatc tgaaagtgaa
     6181 atcacgagga ccatctctgt aaatctaagg agtctgaaat ttcttcaatt tggatctgtt
     6241 atggcgttga ccttagttga gtacagcatg gttgtccgca agcgattccg atacacataa
     6301 attacagcag gcacaattac aggcatttag tgaagtttgt aatgcaaatc tgagatcgag
     6361 cgcaagcgtt cggctgcagc ttcagcggta atatcgcgct gaggcatgac cagcaattcg
     6421 tatcctgcca taaatttacg cacggtggca gaccgaagca atggagggta gaaatgtgcg
     6481 tgaagtatcc attcgggatg agatcgtccg tcaaacgggg cttgatgaaa tcccatggca
     6541 taagggaagg atgattcaaa gagattatcg taacgtgaag taatacgttg gagcgtttcg
     6601 gctaatgccc cgatctcttc gagtgttagt tcactcagcc aggcaacctt tcgatgagca
     6661 ataatcatag tttcgaatgg ccatgccgcc caatatggga caagcacagt aaaatgatcg
     6721 ttcgaaaata aaatccgctt gttgcggact tgctcttcat gcaagtaatc tgctaaaagc
     6781 ggacgaccat gttggctcaa ataagtcgtt tggtactcaa gttcttttgc tggttcatta
     6841 gacagatatt gagttcccca aatctggcaa tgcggatgtg gattggaaca gcccatcatc
     6901 gcgcctttgt tttcaaaaat ctgtacatat ttaacgaaat cttttgcgct aagctccaaa
     6961 gtctgctgtt tccatgtatt tataatgttc tctatttcgc ctagtgcaag ctcgggcaat
     7021 gtcagatcat ggcgaggggt gaaacaaaca actcgacata ttccactctc attggaagat
     7081 gtaagaagag aatgatcaac cgtttgaaca aaattttcgg ctggcaagag cgacgaaaag
     7141 tcgttatcga aaataaatgt ggatgaataa tcaggatttc ttgtacctcc gactctttga
     7201 tttcgtggac atagataaca gtctgggtcg tattccggca gactttcaag aggaacactt
     7261 tctacttgac catgccacgg gcggttggcg cgttgaggcg agaccagaac ccattcgccg
     7321 gtcaggggat tcaatcgacg atgtgaagaa gtttccatga tgtgaaacaa tccgtttaat
     7381 ttagtatggt caccggagat tcattttact cgacaaagtg atgctttgta tgatgtggtc
     7441 gtcaggtcag tgacgttgct ttgctcttaa ttttgcgtgc ttacaacctt taagaagagg
     7501 gtatgctaac gggttatttt cagccaaaac gatattagaa gatctcataa agtctaaaaa
     7561 aattcgcacg tactgataaa gaccaaaatt gtaatcaaaa tttataacct tcaaaaacaa
     7621 gttaagtaag agcctcttgc tacagaattt aaactgctaa caagaatcgc agctttaaga
     7681 gaagaacttt ttgctgaaat caaatccgtt acctttttac cagttcgccg tcttgcatac
     7741 gaataacaag tatcgagaag taagaaattc agttcaaaac agaatgtaac ctattacgac
     7801 ttatatgacc tgttttgcga ctagttatga ccatgcgtat gctaggataa aaaggttatc
     7861 agaccatctg ggtcttattt tttttcaaca ccaaaataca ggtaaaggcc aattcaatga
     7921 actgctggta tgtgatgcaa agcaagcctc gaaaagaagt gttattgcgc gaacgccttc
     7981 gaattcaaag aatcgaagcg tacttgccgt cgatcagagt aaatcccgtg aatccacggg
     8041 ccaggaaaga acagccgttt ttcccgggtt atctttttat tcacgcagat ttggagaaat
     8101 ttccaatttc tgaatttcgg cgtatacctg gttcgatcgg gttggtatgc tatggaggtg
     8161 aaccggctca tattggcgat gcgctgattc gagaaattca aaaacaggtc gaagagattt
     8221 ctgacctgac taccaattat gttgaccgat tcagacctgg ggacttggtt gtgattaacg
     8281 acgggccgtt tgcctcttac aaagcaatgt tcgattgccg attgtccggt tctgatcgag
     8341 tgcgtgtact tttagagtta ctccaagatc agaagatgcg tgtagaactg tccggcaagc
     8401 aattgcagct tttgacgtcg tagtgacgct cgctcgccca accgaataac agtttcggct
     8461 gatcacagcc tcaatagaat tcgaaatgat tgtcggaatg gcggagcatt ggtcttgaaa
     8521 gacaattatt catatcatta aaaaatcctg tagtagcggt atttctcctc tctaagaggg
     8581 tgattattta tgagcatcca aacacaaatc gaacattcct ctatcattgc cgcctccaaa
     8641 aagatcaagg ctgccagttc aattatcgct ccacacttgc aatggaaact ttatatcact
     8701 ggccttatga cctctgatct gttggtaacc tttgccgcat tttggctgtc ctataattct
     8761 cgttttgagt ggtttgatca atattttgtt ccaaccgaag gtgtaccttt tgccacatat
     8821 agatttctac tctatacagt tccttttctt tggcttgtga tcttcgcgat taatggtttg
     8881 tacattagaa gcaatttact gggtggtact cgggagtatt cgaaagtttt tagttctgca
     8941 acaaccggct ttttattaat tattatagta agtttccttg atccgacgct gataattgca
     9001 cgcggctggc tgctattgac atggggtagc acatttcttc ttgtaacgct ggcccgtctg
     9061 ctattaagaa gaattgtgta tagacttcgg cgacatggct tctttgtgac gccagcagta
     9121 attgtcgggg caaatgagga agggcgctcg cttgcggaac aactgcggcg ttggaagtcg
     9181 tcaggactgc atctggtggg ttttgtcgat aagaagacac cagcaacaat tccagtattt
     9241 caccaacttg cctgtttggg ttcggttgat cagttgggag agataatatc gaggtatcag
     9301 atcggtgaag tgatactggc gtctagtgca ttctcaactc gtgattattt gctggatatt
     9361 ttcagagcgt acggcttaac ggacagggtt aatattcgta tgtcttccgg cttatatgag
     9421 atcctgacaa cgagtttgac agttagtgaa tatgcttatg tgccattggt ttacgttaat
     9481 aaggttcgcc tgaccggctc cgatgccttt ctgaaattga tctttgatta tattctcact
     9541 ttggctggtc tcataattct cttgcccttc attcttttga ttgcaatttg gataaaaata
     9601 agctccccag gtcctgtttt tcataagaga ttagtgatgg gactcaatgg aaaacgattt
     9661 tatgccttga aatttcgaac aatggcgatc aatggaaatg agattttaga acaatatcct
     9721 gaattaaagg atgaattggc aagaaatcat aaattgaaaa ccgatcctcg cattactcag
     9781 attggtgctt ttttacgcaa atacagtctg gatgaactgc ctcaattggt taatgttgtc
     9841 aaacgtgaaa tgtccctagt tggtccccga atgatttcac cggaagaggt atcaatgtat
     9901 aagcaatttg acatgaacct tctgactgtt ttgccgggga tcacaggact atggcaagtc
     9961 agtggacgat cggatatttc ctatgaagaa cgtgtacgct tggatatgta ctatgtccgt
    10021 aattggagtc tatggcttga tttacagctt attttccaga cacttcctgc cgtgctcagg
    10081 gctaagggcg catattaatt cgtaaatttg gcgtattaat tatcttttaa gagcttggga
    10141 gaaaacccag tgatcattgt tcagacacca ttgcgtgtca gcttcttcgg tggaggtacg
    10201 gattttcgtg cattctttat gcaggagggc ggatgtgtcc taagctctgc aattgataaa
    10261 tatattttca tcaccattaa gaaacgattt gataacaaac tgcgcatagg ctatacacag
    10321 actgaaatgg tagatgaagt tgatcagatc catcatgagc taattcgaga gtctctacga
    10381 attacaagaa tcagtcatgg agtcgaaatt acaacgatgg gagatattcc ctcagaaggc
    10441 tcaggtctgg gctcgtctag tactgtgacg gttggtgctt tacatgcaat gtatgcctat
    10501 ttgggagaga tggtgcccgc cgaacgactg gcgcatgagg catgtgaagt agaaattgaa
    10561 attctaaaac ggccaatagg tatacaggat cagtatattg ctgcctatgg aggtcttcgt
    10621 tttttcgagt tcttgcctga tggaagggtt actgcagaaa gcattaaact ttcgccagaa
    10681 gcacaaaggg cgctgaacaa caacttcatg ttattcttca cagggataag ccgtacctct
    10741 tcttcaatac ttaaggaaca ggtcaataat ctcaaggacc gcctaagtga attgcgcgag
    10801 atcaaaaaca tggctcgtga agcgcgattt gctatagaat cagagaattt tgacaccctg
    10861 ggcgtcttgt tacaccaatc ctgggagttg aaaaaacgcc tggcaggaac cattagcaac
    10921 ggccaaatca atgaaatgta tgaagcgtcg cgcagagccg gtgcgattgg cggcaaggtc
    10981 gcgggagcag gcggcggagg ttttcttctg ctgcatgtgc ctattgaacg ccaggacaaa
    11041 gttcgagcag cattacatgg cttacaggag cttccctttc gattggaagc cgatgggacg
    11101 aaagtaatct tcaattatcg acgcttataa ggctgattaa ggattaacca cgatggaata
    11161 cattcaagag tacataacaa cattacaact caccatggat cagcttcctc gccagttaat
    11221 agctgatgtc attgacgtgt tgcagcaggc tcgtcgtaag ggaagccagg tatttatttt
    11281 ggggaatggc ggttctgcat cgactgcctc tcattttgca tgtgatctgg caaagaatac
    11341 gcgtcaaaca ggtcttccac attttcgcgt aattggtctc acagataata tggcaatgtt
    11401 ctcagcactc gccaatgatg aaggatacga gaatgtgttt tcggagcagt tggctggtct
    11461 agcccgacct ggggatattg ttatagcaat ttcagccagt ggaaattcta aaaatgtaat
    11521 ttacgccgcg gaagccgccc atcgatacga ggcaacagtc attggattta ctggtttcga
    11581 tggaggacgt ttggcacaat tggcggatat caatattcat gtaaatagta atgttattga
    11641 acatgtagag gacattcacc tgatgctgga gcatatgatt gtcaaaacga tcaaagaaca
    11701 ggttcaaaca tcggtaatta tgaccacttc acttgctcag attccagcag aaaatttcgg
    11761 tgactaagaa tgcgagcgta ttgctggatc atcatcgata gaaaagatcg ctttaagggc
    11821 taaagctaaa ttgtcaaaaa ccaaattttc tgagattttg tccggctggc cttgtagcaa
    11881 ctggttaata ccacggccgg tttttagaag aatcgtgcct cgcgtgcctg ccgcttctcc
    11941 agcttgtata tccgtccatg catcaccaat catccatgag cgccggagat ctaatgacag
    12001 atcgtgggca gcttgtagta ataaacccgg taatggcttg cgacaagaac aaccatcttc
    12061 gggtcgatgt gggcacatgt agacactgtc gatttgcccg ccatgatcgt ggatcaattc
    12121 gattagtctt tggttgattt cttgagcagt tttaagcaag agaagccctc gacctacagc
    12181 agactggttg gtgacgataa cgactttgta ttttttcatc ggagcaagag ataaggcacg
    12241 gatggcttct gggataattt tgacctgtga ccaatctcga acataatccg accggttctc
    12301 gattagtaca ccgtcacgat caagaaatat tgatgggtac atagggttat tatacattcg
    12361 ggcccaatgt accaggatgc acatatcttt ttactgctta cactcgaccc gatgcaaatc
    12421 gcattcgtgt cggaatagcg gagcttgcca actgcaacaa tgctgaaata catacgacta
    12481 taccttgatc gtcaggcctc ttcccttctt cgttattttt atgagcaagc ctttttggtc
    12541 tcattgagct ggatacccac tgttgtcgga attggattgc gtggagtttt ctatcctttc
    12601 attctgcaga tggaagggct tgctgctatc gaaaataatg tacggttgcg atttgctgat
    12661 cacatcaggc ttggccatgg t
//
LOCUS       DASTJP010000416         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_367405, whole genome shotgun sequence.
ACCESSION   DASTJP010000416 DASTJP010000000
VERSION     DASTJP010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367405"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFQ23_12045"
     CDS             complement(<1..551)
                     /locus_tag="VFQ23_12045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9907373.1"
                     /translation="MTDQYFVAVIGAGPAGLFGARELVNLGARVALFNRDIKPGGLAE
                     YGIYPNKYTMKNGLRKQFRQIMDLPNLDYYGNVTVGSHGDLTLDDLRGLGFQAILVTA
                     GAQGTKWLGLPGEELEGVYHAKDVVYYYNNLPPYSQKSFRFGKRCAVIGAGNVMLDIA
                     HFLSREEKVEEVVAVVRRGPSEI"
     gene            909..1667
                     /locus_tag="VFQ23_12050"
     CDS             909..1667
                     /locus_tag="VFQ23_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907374.1"
                     /translation="MTQPVSNQNEPSKLKQLLNGGVDESIKSRLPRAEGMRAAPPEPI
                     AIPVEETESTPAAEPEPKRRQFKFLPAFWTIASVMSFTVNIVMLIVVLVLLQMLGTIQ
                     FTAGDQISGLLGGLYNNFVKMDEASIRTSIHVEKEIPVNFTLNVSGPTNVTLSSDVTI
                     DGALVTVQTGGLNIVNARARIVLPAGSLLPINIENLAVPVDQKVLAVLDVPVDIPLNQ
                     TELHEPFVGLQQVVKPWYCLIQPNALLNNVQVCR"
     gene            1695..>2827
                     /locus_tag="VFQ23_12055"
     CDS             1695..>2827
                     /locus_tag="VFQ23_12055"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9907375.1"
                     /translation="MTKNHLTQISLSNGLKVLLKEIHTAPIISSWLWYRVGSRDEVQG
                     RTGISHWVEHMQFKGTPQFPSNVLDKTISREGGSWNAFTYMDWTAYFETMPADKIDIA
                     LRLESDRMINSQFDPNEVASERTVIISEREGNENEPLFLLGEAIQQAAFRIHPYHHEV
                     IGDMADLHTITRDDLYNHYRAFYVPNNAVMAVAGDFDTQTMLGRIKELFEPIPSGSEP
                     ARLARDEPTQKGEVRLSVEGPGSTTYVQVCYRFPSASHPDFYPLSVLDSLLAGPSNIN
                     MFSGGISNKTSRLYRALVDKEFAVSIHGGAQATIDPFLYSFSMTVHPKRKPEEALAAL
                     DKEIQRVKEEKVSKQEITRAIKQARALFAYGSENITNQGFWLG"
BASE COUNT          741 a          726 c          682 g          678 t
ORIGIN      
        1 ttgatttcag acggaccgcg tcgaacgacc gcaacaactt cctccacctt ttcttcacgg
       61 ctgagaaagt gagcgatatc gagcatgaca ttgccggcac caattacagc acagcgtttt
      121 ccaaagcgga atgatttctg actgtacgga ggcagattgt tgtaatagta cacgacgtct
      181 tttgcatgat agaccccttc gagctcttcc ccgggcaggc ccagccactt ggtaccctgc
      241 gcgccggcag tcaccaggat cgcctggaag ccaagccctc gaagatcgtc gagtgtgaga
      301 tctccgtgtg agccaaccgt cacatttcca taatagtcaa gattgggcag atccatgatc
      361 tgacggaact gcttgcgcaa gccatttttc atggtgtact tgtttgggta aattccatat
      421 tcagcaagcc cacctggctt gatatcgcgg ttgaacaacg caacccgcgc tcccagattg
      481 acaagttcgc gcgcaccaaa taaacccgcg ggtccagcgc cgatcacggc tacgaagtat
      541 tgatctgtca caatgcctcc gaaagtttct accctaaata ataagtgcgg gctgattata
      601 gctaaaagga aattcaccta caagacggtc tctcattctt ttggtaacat aaaaccagga
      661 ttgccgaaaa agcgtctctc gaaatactat atctatgtta agattgcggc gtagtggcca
      721 cacgtggcat cggctcttct accttttcag tgaatttcca gtctataatt tacctggaaa
      781 agtttagcaa atgatgagat cagcatagca cgccgcccgg cggtagtgcg gacaagccat
      841 cacttactac ttgtcagtag cagacagatt caaaatgcaa gcagtcgttc tctgatcgag
      901 gaggtcttat gactcaacca gtttccaatc aaaacgagcc cagcaaactg aaacaactgc
      961 tgaacggtgg agtggacgaa tccatcaaat cacgcctgcc gcgggcagag ggaatgcgcg
     1021 ccgcgccacc ggaaccgata gcaattccag ttgaagaaac tgagtccacg ccggcggctg
     1081 agccagaacc caaacgacga caattcaagt tccttccggc attctggacg atcgccagcg
     1141 tgatgtcgtt taccgtgaat atcgtaatgt tgatcgtggt actggttttg ctccagatgc
     1201 ttggaacgat tcaatttacc gcaggtgatc aaatctccgg tttgcttggc ggtctctaca
     1261 acaacttcgt caaaatggac gaggcaagta tacgaaccag tatccacgtc gaaaaagaga
     1321 tccccgttaa cttcacgctg aatgttagtg gaccgacaaa tgtcacgtta agtagtgacg
     1381 tcacaattga tggcgccctg gtcactgtgc agacaggtgg cttgaacatc gtaaatgccc
     1441 gggcaaggat tgtccttcca gcaggttcgt tattgccgat caatattgaa aatctggctg
     1501 tcccggtcga ccagaaagtt ttggcagtgc tcgatgtccc tgtggatatt ccactcaatc
     1561 agacggaact ccacgaaccc ttcgttggtt tgcaacaggt ggttaagccc tggtactgcc
     1621 tcattcagcc aaatgctttg ttaaataatg tgcaggtttg ccggtaactc aactgactca
     1681 tggagtaagc aaatttgacg aaaaatcatc ttacacaaat cagtctgtct aacggattga
     1741 aagtattgct gaaggagatt cataccgccc cgatcatttc gtcatggctg tggtatcggg
     1801 tgggttcacg cgatgaggtg caaggtcgaa cgggtatctc acactgggtg gagcatatgc
     1861 aattcaaagg caccccacaa ttcccatcca atgtgctgga taagaccatc tctcgggaag
     1921 gcggctcgtg gaatgcattc acgtacatgg attggaccgc ctactttgaa accatgcctg
     1981 ccgacaaaat cgacatcgct ctgcgtctcg agtcggatcg gatgatcaac agccagttcg
     2041 atccaaacga ggtggcttct gaacgtaccg taatcatctc ggagcgggag ggcaacgaga
     2101 atgagccact ttttcttctg ggtgaggcta ttcaacaagc ggcatttcga attcatccat
     2161 atcatcacga agtcatcggc gacatggcag atctccatac aattacccgc gatgatcttt
     2221 acaatcatta tcgtgcgttc tatgtgccga ataatgctgt gatggcggtc gcaggtgatt
     2281 ttgatacgca aaccatgttg ggtcgaatta aagaattgtt tgaaccgatc ccttccggaa
     2341 gtgaacccgc gcggctcgcc cgtgacgaac cgacacaaaa aggagaagtg cgtctgtcag
     2401 tggaaggacc cggctcaacg acctacgtcc aggtttgcta ccgtttccct tccgcatcgc
     2461 atcccgattt ctacccccta agcgtgctgg acagtttact tgccggcccc agcaacatca
     2521 acatgtttag cggcggtatc tcgaataaga cctctcgtct gtatcgtgca cttgtggata
     2581 aggaattcgc ggtcagtatt catggtggtg cccaggcgac aattgatcca ttcttatatt
     2641 cattctcaat gaccgttcat cccaaacgca aaccggaaga agccctggct gctttggata
     2701 aggaaattca acgggtaaaa gaagagaaag tctcaaagca ggaaatcact cgcgcgatca
     2761 aacaagcgcg cgccctgttc gcctacggca gtgagaacat caccaaccag ggattctggc
     2821 tgggtta
//
LOCUS       DASTJP010000417         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1029209, whole genome shotgun sequence.
ACCESSION   DASTJP010000417 DASTJP010000000
VERSION     DASTJP010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029209"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VFQ23_12060"
     CDS             complement(<1..1194)
                     /locus_tag="VFQ23_12060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9907376.1"
                     /translation="MKLFSSDQSTMDQIAKADLAHVLIMQLDDSSAVVMLSPALRALR
                     EALPHAQLTLLTTEAGSQMAPLLPWVDHVMVDQAMWQDGTGSRLINPREEIAFIERLR
                     RHDFSIALIFSSVSQSPLRAAFACYLAGIPYRVGFAKGMSGSILSHSLLPPADELHQV
                     DRNLNLLRAIGISEADQRMELNIPQNVENRANELLGLAGLKLNTPYVVLAPGSMGTLG
                     PYAPDHFAAVARILAAQTEQQLVIVGNSAEAKTIQPVLKVVHENLYGNVHSLVEKTTL
                     PELAAIIRQANLTIANQSSVSMHLADVFRCPMVILHSEMDTVNQWMPRNSSVRLLSRP
                     AACAHCNQVNCMHGINCLDVRPEEVAIAALELLTKQTYNQSEYKGIFNYKMEAERNQQ
                     PSAREI"
     gene            1508..2491
                     /locus_tag="VFQ23_12065"
     CDS             1508..2491
                     /locus_tag="VFQ23_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907377.1"
                     /translation="MNSTTDRNRSGDVVQAQDLTKMFGEEAAVDSLTMHVPRGTIFGF
                     IGPSGCGKTTTVRLLTGVHKPTSGQVTVLGKNPYDFSKSDREKIGYLIQNFVLYPELT
                     VWENLNFAASFYGVSLLGRAKRFNHLLDFVDLKEDKGKLASALSGGMKRRLSLAATLV
                     HNPELLFLDEPTAGIDPLLRRKFWDYFKELQNEGHTLFVTTQYVGEAAYCDLVGVMYD
                     GRLLMVETPDGLRKKAFGGEIVSIKTSEWIKFESRKKLESLPFVHGKIKVISDQEIEV
                     IVDEASTAMPALIEACKAERIELETIEEISPPFDDVFVKLIEMETNNEKVV"
     gene            2475..>2743
                     /locus_tag="VFQ23_12070"
     CDS             2475..>2743
                     /locus_tag="VFQ23_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907378.1"
                     /translation="MRRLSKFLIRISSFVSKELTEILRQPRLILTLVFGPFLIMFLFG
                     LGYPEQNRTLRTTFVAEDPSALQGNMELFTGSASPAIADQDIESD"
BASE COUNT          708 a          662 c          714 g          659 t
ORIGIN      
        1 tatttcgcga gcggaaggct gctgattcct ctctgcttcc attttataat tgaatattcc
       61 cttatattca gattggttat aggtttgttt ggtcagtaat tccagggccg cgatggcaac
      121 ttcctcagga cgaacatcga ggcagttgat cccgtgcata cagtttacct ggttgcagtg
      181 tgcgcaggct gcaggccggc tcaacaatcg cacagaggaa ttgcgcggca tccactggtt
      241 gaccgtgtcc atctcagaat gcaggatgac catggggcag cggaatacat ctgccagatg
      301 catgctaacc gatgattgat ttgcaatcgt caggtttgcc tgccgaataa tggctgccag
      361 ttccgggagc gtcgtctttt cgaccagcga atgcacattc ccatacaaat tttcatgtac
      421 caccttgaga acaggttgga tcgtttttgc ctctgccgag tttccaacaa tgacaagctg
      481 ctgttctgtt tgggcagcca ggatgcgagc tacagctgcg aaatgatcgg gagcatacgg
      541 tcccagagtc cccatagaac ccggcgccag cacgacatag ggagtgttca gctttaagcc
      601 tgcaagaccc agcaattcat tggctctgtt ttccacattc tgcgggatat tcagttccat
      661 ccgttgatct gcctcagaga tgccaattgc ccggagcagg ttgaggttcc tgtccacctg
      721 atgaagctca tccgccgggg gcagcaggga atgagaaaga atggagccgc tcattccctt
      781 cgcgaaccca acgcgataag gaatgcctgc cagatagcag gcaaaggcag cccgtagcgg
      841 cgactgcgaa acactactaa agatcaaggc tatggaaaag tcatgacggc gcaatcgttc
      901 aataaaagca atttcttccc gggggttaat caaacggctt ccagtaccgt cctgccacat
      961 cgcctgatca accatcacat gatccaccca cggcagcagc ggagccatct ggctgcctgc
     1021 ctccgtggtc aggagcgtca attgagcatg aggtagagcc tcgcgtaacg cgcgtagcgc
     1081 aggactgagc atgacaactg cgctgctatc gtccagctgc atgatcagca cgtgcgccag
     1141 atcagctttt gcgatctgat ccatggttga ttggtctgag ctaaaaagtt tcataacacc
     1201 ttcatatctt cagcatcccg ataacgtcct gccaactata tgattacttt tcattcttat
     1261 caagcaagaa tgtcccctga aacaggcagg gttttcccgt aacgaagaat cgcatcagaa
     1321 tagtgttttt ggttttgcaa atcaattagg gaaatcccta accatggata gaaacatccc
     1381 ttattgatgg ggttgaaggc gatgataata tttacagtgc gggtaacaaa acatgtgttg
     1441 ggttagcaag acaaacggat atctcgaaca atcattgttc gatcttcaaa acataagata
     1501 ggaaaaaatg aactctacaa ccgacagaaa tcgttcaggg gatgttgtgc aagcacagga
     1561 tttgaccaag atgtttggag aagaggcggc cgtcgattct ctgaccatgc acgtgccgcg
     1621 tggcaccatt tttggtttta ttggtcccag cggatgtgga aaaacaacaa cggttcgctt
     1681 gctgaccggg gtccacaaac cgacatccgg acaggtgact gttctgggta agaaccccta
     1741 tgatttttcc aaatcagacc gggaaaaaat cggctatttg attcaaaact ttgtcctgta
     1801 tccggaactt acggtgtggg aaaacctgaa ttttgccgct tctttttatg gcgtgagcct
     1861 tttgggtcgg gcgaaacggt ttaaccacct cctggatttt gtggacctga aggaagataa
     1921 aggaaaactg gcgtcggcgc tctcgggtgg catgaagaga cgcttgagcc tggcggccac
     1981 cctggtgcac aatcccgaac tcctgtttct ggacgagcca accgcgggca tagacccgct
     2041 tctacgacgc aagttctggg attatttcaa ggaacttcaa aacgaaggtc atacgctatt
     2101 cgtcaccacc cagtatgtag gcgaagctgc ctactgcgac ctggtaggcg tgatgtacga
     2161 tggaaggctt ttgatggtgg aaactcccga tgggttgcgt aagaaggcct ttggtgggga
     2221 gatcgtgagc atcaaaacct ccgaatggat aaagtttgaa agccggaaaa agctggaaag
     2281 cctgcccttt gtgcacggaa agatcaaagt gatctccgat caggagattg aggtcattgt
     2341 ggacgaggca agcacagcga tgcctgctct gatcgaggcg tgtaaagcgg aacgaatcga
     2401 attggaaaca atcgaagaga tatccccacc tttcgacgat gtatttgtga agctgataga
     2461 aatggagaca aacaatgaga aggttgtcta agttcctgat ccgaatctcc tccttcgtga
     2521 gcaaggagct gacggagatt ctgcgacagc cgcggctcat cctcaccctg gtgttcggtc
     2581 cgtttttgat catgttttta ttcggattgg gctatccgga gcaaaaccgc acactgagga
     2641 caaccttcgt tgcggaagat ccgagcgctc tccaggggaa tatggaattg ttcacaggct
     2701 cagcaagccc cgcgatcgcg gaccaggata tagaaagtga caa
//
LOCUS       DASTJP010000418         4625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1348485, whole genome shotgun sequence.
ACCESSION   DASTJP010000418 DASTJP010000000
VERSION     DASTJP010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348485"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VFQ23_12075"
     CDS             complement(<1..1214)
                     /locus_tag="VFQ23_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907379.1"
                     /translation="MNQLKINSNSMRLLLMVCLCAQLVMFSPVKAQELMQQNNLPAAA
                     PNVGSCPMFPENNIWNTAIDNLPIHTRSDQWVNSIGRNTGFHMDFGSGTWNGGPIGIP
                     YNIVGGSVPKVPVSFYYPGESDPGPYPIPSNPLIEHGSDHHILMVDNSTCTLYELFDA
                     SYSGGSWHAGSGAIWNLNSNALRPDTWTSADAAGLPILPGLVRYDEILSGEINHAIRF
                     TASNTNGYIWPARHLTSNNPNTPYIPPMGARFRLKASFNVSSYPPAMQVILNAMKKYG
                     IILADNGSNWYVSGAPDSRWNNDMLHLLDDLTGNDFEAVNISQLMVNYNSGATGAIIS
                     GNVDLQGVRLSYDDGGARTVTSSEDGSYSFPVSYNWSGTVTPTHPCFTFNPPSVTYSN
                     VTTNQTRQNYTAT"
     gene            complement(1459..4578)
                     /locus_tag="VFQ23_12080"
     CDS             complement(1459..4578)
                     /locus_tag="VFQ23_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907380.1"
                     /translation="MINFLKTKTVIIRIFTLMALILPVLIPVKSWVLAENEVRYFSSD
                     NATYTSDFGVENPEGMAYFSGTNSFVVWGMNKNERMISLRTETSKKTNFTELITNPLA
                     ASFDNRSNNLFSLDAGNKELQKIKVDSKNAQPLLNESARFKIESLNLSNIDGMAVDPA
                     TGQLFVLDAGSQQILALSPDSANGSTYQDAANYLSLANLSKSGLRGVAFNPTNSHLYV
                     GDPTARKVYEVTKTGDLVSTYDISYLELKSPSAMLFAPSQDATDDPNIMSLYILDSGQ
                     PVQSDANIAADQRNTSERGQIVELSLQAPAALPSGTTLLPATLVRTFDTSNQAWNPSS
                     PDPAGIDYWPAANRLVISDSEVEEMRPYWQGSNVFLSTTSGNLTGTCSTMAYTGEPTG
                     VAVNPNNNHIFIAADYQDLLFEISLGQDGQYCTSDDVVTETSLVNAYGVTDAEDVAYG
                     NNTVFIAGGDDAEVFVIPLGADGVVGGGDDGPMTHWDTNSLGFADAEGIGFNHDAGTL
                     FIVSTKRTDRYLGEFSTSGTLLKAYNLSFMPDVGNIRSDVTYAPGSQNASVKNIYIAS
                     RGIDNDSDRTENDGKVWEIAISNAVVPTATTVIVPTATNVTVPTPTNVVVPTPTTASG
                     SANVQVNIGNNHVGGFDIPHVGSINPSFVANNGPVKITSSNNVPILASMQAIWREPGY
                     RSSYSELMGLPKEQLSTEYWFPWYNNLYPHIMDQGFRIANINATSPNTIEIWMGNTLL
                     DRFDLGIAASTRVGYAVDNGPIRVICTTCTGNEKILAAMRVIWREPGQRTSYSELMGL
                     PKEQLSTEYWFPWYNNAVPASLDQGFRIANVDSSTHTIQVRVGTTLLDSFALDAGASV
                     RTSYFIDNGPIRVVCTDCGGGGKILAAMRVIWQEPGFRFSYSELMGTPKEQLSKEYWF
                     PWYNNAAPASMDQGFRIANVDTSIHTIQVWAGTTLLENFTLTGGASARKAYTVANGPV
                     RIICTTCTSSGKILAAMRVIWQEPGFRSSYTEMMGLPVEALSTEYWFPWYNNVDTPSM
                     NQTFRISVP"
BASE COUNT         1178 a         1087 c         1114 g         1246 t
ORIGIN      
        1 gtcgctgtgt agttttgtcg cgtttggttg gttgtcacat tgctgtacgt cacactcgga
       61 gggttgaacg tgaagcaggg atgcgtgggt gtcaccgtcc cgctccagtt gtatgacacc
      121 gggaaggaat agctgccatc ctcagacgat gttactgtcc ttgctccccc gtcgtcatag
      181 ctcaacctca cgccctgtaa atctacgttt ccagaaatta tggctccagt cgcccctgaa
      241 ttgtagttaa ccataagttg agatatattc acagcttcaa aatcgttacc cgttaagtca
      301 tcgaggagat gcaacatatc gttattccaa cgcgaatctg gtgcacctga aacgtaccag
      361 ttcgatccgt tatctgccag gatgatccca tattttttca ttgcattcaa gatgacctgc
      421 attgctggtg gatagcttga aacattaaac gaagctttta accgaaaccg cgctcccatc
      481 ggaggaatat agggtgtatt gggattattg gatgttagat gccgcgcagg ccagatgtaa
      541 ccgtttgtgt tcgaagcagt aaagcggatg gcatgattaa tttcaccact gagaatctcg
      601 tcgtagcgca ccaaaccagg cagaattggt agtcctgctg catctgcaga tgtccatgta
      661 tcgggacgca atgcgtttga gttcaagttc cagatcgctc ctgaacccgc atgccacgaa
      721 ccgcctgaat aacttgcatc gaatagctca taaagtgtgc aagtgctgtt atccaccatc
      781 agaatatgat ggtcactacc atgttcgatc aagggattcg atggaatcgg atacggtccc
      841 gggtctgatt cacctggata gtaaaaagaa acaggaactt tgggaacgct acctcctacg
      901 atgttatatg ggataccgat tggacctcca ttccaggtgc ctgaaccaaa atccatatgg
      961 aagcctgtat ttcgtccgat ggaattgacc cattgatcag agcgtgtatg aataggcaaa
     1021 ttgtctatgg cggtattcca gatgttattt tccgggaaca ttggacagct accaacattg
     1081 ggggcagcgg caggtagatt gttttgctgc ataagttcct gtgctttgac tggactaaac
     1141 atcacaagct gtgcacataa gcagaccatt agtaaaagac gcatactatt tgaattgatc
     1201 ttcaattgat tcataataac ctctggatga tgctaacata tcgaccactt cagtcaatat
     1261 tttgtcatct cgggcaatac gtcaattttg taaggaatgg tttccatcaa ggtgaaaatg
     1321 ttattagcaa tacgtgagcg aaccaaacga atattcgatc gtttcttgaa atctcttaaa
     1381 gagttgaaac tgcttgcaga gaaagaaaat acgggcaatt acaattgccc gtattttccg
     1441 tgaagacagt aaaagttatt agggtacgct gattctaaat gtttggttca ttgagggcgt
     1501 atcaacgttg ttgtaccagg ggaaccaata ttctgtggaa agtgcctcaa caggcagacc
     1561 catcatctca gtgtagctcg aacgaaaccc gggttcctgc cagatcactc gcattgccgc
     1621 caggattttt ccgctgctgg tgcaggtggt gcagatgatt cggaccggac catttgctac
     1681 agtatatgcc ttacgtgcac tagctccgcc agtaagggtg aaattttcca gtaatgtagt
     1741 gcctgcccaa acttgaatcg tatggatgga tgtgtccaca ttcgcaatgc ggaagccctg
     1801 atccatagaa gcaggggcgg cattgttata ccaggggaac cagtattctt ttgaaagctg
     1861 ttcttttggc gtccccatta gctcagagta actgaaacga aacccgggtt cctgccagat
     1921 cactcgcatt gccgccagga tttttccgcc gccaccgcag tctgtgcaaa caacgcggat
     1981 tggaccatta tcaatgaaat agcttgtcct tacgctcgct ccagcgtcga gtgcgaagct
     2041 atccaggagt gttgtaccga cccgaacctg gattgtgtga gtggaggaat ctacatttgc
     2101 aatgcggaag ccctgatcca gggaagccgg aactgcattg ttgtaccagg ggaaccaata
     2161 ttctgtggag agttgttctt ttggtagtcc catcagttcc gaataactcg tccgctgtcc
     2221 tggctctctc cagatcacgc gcatggcggc caggattttt tcattgcctg tacaggtggt
     2281 acagattaca cgaatcggac cgttatccac agcatagcct actctcgtac ttgcggctat
     2341 gccgagatcg aatctgtcca gtagagtatt gcccatccaa atttcgatcg tattggggct
     2401 ggtagcattg atattcgcga tgcggaaacc ttgatccatg atgtgtgggt agagattgtt
     2461 gtaccagggg aaccagtact cagtggacaa ctgttctttt ggcagaccca tcagttcaga
     2521 atagctggat cgatatcctg gttctctcca gattgcctgc atggaagcca ggatcggaac
     2581 attattcgaa ctggtgattt tcaccggtcc attgttggcg acaaagctcg gatttatgct
     2641 tccaacatgg gggatgtcaa aaccgccaac atgattattt ccaatattta cttgtacatt
     2701 tgcagaaccg gaagcagttg tcggagttgg cacaacaaca ttagtgggtg tgggcacagt
     2761 aacattggtg gcagtcggaa caatgacagt agtggccgtg ggcacaacag cattggaaat
     2821 ggcgatttcc cacacttttc catcgttctc agttcgatcg ctatcattat caataccgcg
     2881 actggcaata tagatgtttt tgacagaggc gttctgactt cccggggcat aggttacatc
     2941 cgaacggata ttgcctacat ccggcatgaa cgataagtta taggccttca gcagcgttcc
     3001 cgaggtggaa aactcaccca gatacctatc tgtgcgtttt gtactgacga tgaatagcgt
     3061 gccagcatca tggttaaagc cgattccctc cgcgtcggca aagcccagag agtttgtgtc
     3121 ccaatgggtc attggaccat catctcctcc gccaactacg ccgtctgccc cgagcggaat
     3181 gacaaacact tccgcatcat caccaccggc aatgaagacc gtgttattcc cataggctac
     3241 atcttcagca tcggtcacac cgtaggcgtt gacaaggctt gtttctgtta ccacatcatc
     3301 agaggtgcag tactgcccat cctgacccag gctgatctcg aacaatagat cctgatagtc
     3361 ggcagcgatg aagatatggt tgttgttcgg gttgactgct accccggtcg gttctccagt
     3421 gtatgccatc gtgctgcatg ttccagtcag attaccggaa gtcgttgata ggaatacgtt
     3481 cgatccctgc cagtaaggcc gcatctcctc cacttcgctg tcgctgatca cgagccgatt
     3541 tgcggcaggc cagtaatcaa tgccagccgg gtctggggaa gaggggttcc aggcctggtt
     3601 ggatgtatca aaggtacgca ccagggtcgc tggcagtaaa gtcgttccgg atggcaaagc
     3661 cgcaggagcc tgcagtgaca gctcgacaat ttgtccccgt tcagaagtgt ttcgttgatc
     3721 agctgctata tttgcgtccg attggacagg ctgtccacta tccagaatat aaagagacat
     3781 aatatttgga tcatctgtcg cgtcctggct gggggcaaac agcatcgcag atggagattt
     3841 caattccaaa tagctgatat cgtatgtgga aaccagatct ccggtttttg tcacttcata
     3901 aacttttcgg gcagtgggat cgccaacgta caaatggctg tttgtgggat tgaaagccac
     3961 accgcgcaag ccggacttgg atagatttgc aagactgaga taattcgcgg cgtcctgata
     4021 cgtagatccg ttcgcagaat ctggagacaa tgccaaaatt tgttggctgc cagcgtccag
     4081 aacaaaaagc tgtccggtag ccgggtcgac tgccatacca tctatattgc tcaaattaag
     4141 ggattcgatt ttgaagcgag cggactcatt aagtaaaggt tgagcgttct tgctatccac
     4201 cttgattttc tgtagttcct tattcccagc gtccaaagag aataaattat tgctacggtt
     4261 atcgaaggat gcagccagcg gattagttat aagctcagta aagtttgtct tcttacttgt
     4321 ttcggtgcgc aaagaaatca tccgttcatt tttattcatt ccccacacca caaatgaatt
     4381 tgtgcctgaa aaatatgcca ttccttcggg attctctaca ccaaaatctg acgtataggt
     4441 tgcattatcc gaagaaaagt accgcacttc attctcagcc aaaacccagg atttcacggg
     4501 tattagcaca ggcaaaatca gtgccatcaa tgtgaaaata cggataataa cagtctttgt
     4561 ttttagaaaa tttatcattc ttcaggtctc ctcattctcc gttcgtagtc tggcgcgtga
     4621 gatca
//
LOCUS       DASTJP010000419        10603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1001568, whole genome shotgun sequence.
ACCESSION   DASTJP010000419 DASTJP010000000
VERSION     DASTJP010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001568"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VFQ23_12085"
     CDS             complement(<1..1207)
                     /locus_tag="VFQ23_12085"
                     /inference="COORDINATES: protein motif:HMM:NF028221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907381.1"
                     /translation="MKRELGAFERALVIADQYAPFHIISVLRVEGAPSPQIVRNALKV
                     LQSQHPFLSARLLQEKGRQYFVKLIDPGLLLRVLPRWNDAHWVQVVEVEMGLRIDVLN
                     GPLFRCIYLYDATHERAEIILTFYPAIADSASIGKFMHDLLKACASFTDEKTVPVRVT
                     SAAPPVESRFPSPYHGLRLALHRVRYELRQISDEIFYRIQTRGKRTPPIHQTSTRGRI
                     LSIQLPRDLLEALLHRTRQEEVSLSSALHAAMILAVHRELYAGQPGPMHLLALINLRP
                     HVEPPLDDEELASYLSMLRQTVQMVGGIDLWTLAQHLQKKMQSSIRAGDHFVAAEFAE
                     SLMQFITERKSFRLSATALNYNEAVTIDAGYGKIKVTAVHGFPPIFDLGPELFAQAHI
                     FNNQLFMDFA"
     gene            complement(1207..2637)
                     /locus_tag="VFQ23_12090"
     CDS             complement(1207..2637)
                     /locus_tag="VFQ23_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9907382.1"
                     /translation="MATATSLNFNDEIPGPSPMPIFGWLPFAFQFAFSPLSTLEDLRR
                     QYGNLIKLKIKNYPVVIAFDPEYNRQVLRDPSAFYSYDLELVPVPFPRESSLARLTTG
                     MPLMNGGRHHDHRSALLPYFHKKFITRYHEGCVITTEKTLASWKIGAKVNMRTEMEQL
                     AMKLATSPVLGLDPERYGEVFGRQLERVMNLIMNPFVLLFPYDIPGMPFRKLLRTANE
                     MERIVKDVIQHKKEEGLTGDDILSVMIRMHEEDPERLSERELFGHTTTMFRGGYNPNG
                     MALYWTIFLLIQHPEKLRVLLHELDEKLGGNIPTPEQLEDLPYLEGAIKETMRLFPAG
                     TWTGRLAMQPFQLDSHAIPKGTWVVMSPYITHRIPEVFPEPDQFQPERWQSIHKSAYE
                     FMPFSAGPRYCIGTSLGMMQLKIALAMIFQRNSFQLEPGTVVDCIGLNSIRPKNGLPM
                     LVNSYNTDSRKTPFKGNVHKIVRFNP"
     gene            complement(2678..6985)
                     /locus_tag="VFQ23_12095"
     CDS             complement(2678..6985)
                     /locus_tag="VFQ23_12095"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9907383.1"
                     /translation="MSNPALTELLASYVPKLIQNRIVADPRPIESPVTEELPAAILFA
                     DISGFTPLTERLAETGPTGVETLARVLNQYFGQLIDIIHAYGGDIVKFAGDALIAVWP
                     IVSDLPISPQQANINQTSGSKENVVSRADQWQWTMRVAECALKIREQLNNYRVEDSNL
                     YLKLAISTGNITTAHVGGEFNRWEFLLTGAPLVELGIANNLAHAGEIIITPSSWKLIR
                     NDCVAAPLEFELKEAIAQGGLLTGLNKPSSIFSSKTKLDIPAGAERSLRAYIPGAIIN
                     RLSAGQSSWIAELRRVTVLFINLPDLDQNTELMTAQSIAQLIQSSVYRYEGSINKINV
                     DDKGITIVAAFGLPPFAHEDDPTRGVQAALLIRKKLTSRKVRSNIGVTTGRIFCGSIG
                     NEERREYTTIGNAVNLSARLMGAAGMQDELISKYGVPILCDRLTYDSAKDVVEFEALL
                     PQHVKGRTDAVEVFHPLDQKKSVIRPKTELIGRQEEKTMIANALQELARGTEHQTIIL
                     QGEAGIGKSRLFEDLARQAETLNVHMLNGAGDAIERTTPYHAWRPIFNRILDIDTITG
                     GGELTEEDKGAIQDQVLATLNSIDADLVRYAPLLSVVLPIPLPDNELTSAMSGEIRGG
                     NIREVLTRLLAYETAQAPLLIVMEDLHWFDSASWSLLVDVQQKVRPILLALNTRPISD
                     PVPLQFKQLQESSQARLIRLEAMMLDDVEALVCQRLGVKSIPPVIGRLIREKSEGHPF
                     FAEELAYAMREAGILKIENQECQIDPDLNNFEDLTLPDTLQAAITNRIDSLDPSQQLT
                     LKVASVIGRIFALRVLEAVHPIEADKPALQDYMETLTRLSLTLVESETPELAYIFKHA
                     VTQEVAYNLMLYSQRRQLHKAVAEWIEQSNEMNIESYYALLAYHWTQAAEVTEAAEKD
                     YAVYKAVDYLEKAGEKAMQTYANKEAVQFFTQALDWDSKLPKPEDKQASYRRQIRQAR
                     WHGQLGLAHYSLGSLTDCNKHVREALRLLESPIPASRAQFAVGLVPQIIRQIFHRLFP
                     SRYIGSISNEQERQVAIDVSRLYELMSRIYFYSNETLPIMYCILRLLNTAERAGPSAE
                     LASGYAGMAVLSGFAQLHTLAQSYVDRALKVAKAVNQPSNLITVNVVTGVYELGIGKW
                     DSVRAKGLEAQAICEQLGDYRQWGDATVLLAENALISSNIEYALNIQRILLEDAHKRR
                     NPLQQGWGLFGVAANSIRLGNVTEAIPMLEEALQILEELPNLASSINTNGQLALAHLR
                     LGQHGQAVAYASAVLELAAGISPTVYSMDIGFAAVADVYYELWEKALQDSNRHFEADK
                     YELLAEKALKLLVAFQKVFPIGQPVTLYYQGWYEWLTHKPQAAIKTWNKGVEAAKKFN
                     MPYQEGLIRVKLGTSSQEDLHKRREHFDRAIRIFEKMGAMHELTIAQEAGKQAGLQE"
     gene            7145..8071
                     /locus_tag="VFQ23_12100"
     CDS             7145..8071
                     /locus_tag="VFQ23_12100"
                     /inference="COORDINATES: protein motif:HMM:NF019272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET9907384.1"
                     /translation="MNRSLLSLLVLFFFTSSCGPKSQPSPGDTWTQLKSMPTARSENA
                     AAAVGDIIYVSGGFGGEQKLEAYDTTENTWRTLADLPEPRHHLMSASHNGKVYIFGGA
                     ASIVNWAPRADAWVYEPETDSWSRVALMPERRLAGAAVTLGDYIYVLGGTGGSNALLR
                     YDPAQDEWTKLAQLSQSREHTTATTFDGRLYTLGGRWSGIGELTSVEIYDPASDSWTS
                     AQTARAGFATVTVNGKIYVMGGEVLTGANQALKSVEVFDLASGRWEFAPDLPLALHGV
                     PAVNVDDVIYVLGGADRAGAISNQGLVFSFTP"
     gene            8145..8465
                     /locus_tag="VFQ23_12105"
     CDS             8145..8465
                     /locus_tag="VFQ23_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907385.1"
                     /translation="MQSFNINDAVSFRLATQQEDTGAKMQVFFGVLLGVILRWIVVDV
                     IKNQVPQNIPVIIARVVVALIVTIFVFSGYWQKVKEQPPGMRFLNALAYGVSIDALVG
                     PWAF"
     gene            8478..8828
                     /locus_tag="VFQ23_12110"
     CDS             8478..8828
                     /locus_tag="VFQ23_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907386.1"
                     /translation="MDSFSFNDALRFRLATQQEDTNAKIQVFIGVFLGVILRWIYSVA
                     VDVIRNGGSWCFGSLTNVLIRLAVALGITFFVFSGYWQKVKDQPASMRFWNAVAYGIS
                     IDVIVSSWLPSGPC"
     gene            complement(8906..9226)
                     /locus_tag="VFQ23_12115"
     CDS             complement(8906..9226)
                     /locus_tag="VFQ23_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9907387.1"
                     /translation="MPVYVYRCASCGHQFERHQFFHDEPLKICPSCRKKTLKKVITPS
                     KVIFKGSGFYSTDNKSPSGDKPSSGNNSSSTSKSKEGKSEKKEESKSSESKSSETKSS
                     ETSE"
     gene            complement(9310..9687)
                     /locus_tag="VFQ23_12120"
     CDS             complement(9310..9687)
                     /locus_tag="VFQ23_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907388.1"
                     /translation="MDTKLLFGIGLMILILSTCSIDATPADSGIEGQVLIGPMCPVVQ
                     EGQECPDQPYQATLTVNSLSGKKIVQVQTDEQGHFKIPLMPGQYILHPESLNGIPYAG
                     EQTFRVEAGAFTQIIVNYDSGMR"
     gene            complement(9803..>10603)
                     /locus_tag="VFQ23_12125"
     CDS             complement(9803..>10603)
                     /locus_tag="VFQ23_12125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9907389.1"
                     /translation="TATVFISPTSTPTETLTPVPTATLAPQWVMRGPNEVTVPILLYH
                     HIGIPKSESLYYVPPYEFERQMFLLREWGYQTISVELLVRAINQGAELPPKPIILTFD
                     DGGEGTFINALPILQKYGFTGTAYVVHNYIGIGGYMNAEQIRTLYAAGWEIGSHSVSH
                     VDLTVRTDRQKDEIVDSRIKLQAMLGVPILTFAYPFGAYNTDSVHYAHFAGYIGAVGL
                     GNESLQGSRNLFYLYRRTVSGTQTLESFALLLPWREDLSILPAITIVP"
BASE COUNT         2622 a         2610 c         2608 g         2763 t
ORIGIN      
        1 aggcaaaatc catgaacaac tgattgttga agatatgagc ctgcgcaaag agttcaggtc
       61 ccagatcaaa gataggcgga aagccgtgca cggcggtgac ttttatcttt ccatagccgg
      121 catcgatcgt cacagcctcg ttgtagttca aagcagtcgc gctcaagcga aacgactttc
      181 gttccgtaat gaactgcatc agcgattctg caaactccgc agccacaaaa tgatcaccag
      241 cacggattga gctttgcatc ttcttttgca gatgctgtgc cagcgtccac aggtcgatgc
      301 cacctaccat ctggactgtc tgccgaagca tggagagata gctggcaagt tcttcatcat
      361 ccaagggtgg ctccacgtgg ggacgcaaat tgataagggc caagaggtgc atgggacctg
      421 gctggcccgc gtatagctct cggtgaaccg ccaggatcat agcggcatgc aaggcactgc
      481 tcagagatac ctcctcctgg cgagtgcggt ggagaagagc ttccagaagg tccctgggta
      541 attggatgga aaggattcgt ccgcgagtgg atgtctgatg gatcggcgga gtccgtttac
      601 cgcgtgtttg aatacgatag aaaatctcgt cgcttatctg gcgcagctca tatcgtacac
      661 ggtgcaaagc cagccgcaat ccatgatagg gagatgggaa acgggattca actggaggcg
      721 cagcagaagt cactctaacg ggaacagttt tctcatccgt gaatgaggcg caagccttca
      781 agagatcgtg catgaacttc ccaatcgagg ccgaatccgc gatcgcggga taaaatgtca
      841 gtattatctc tgcccgttcg tgtgtggcgt catacaagta aatacagcga aatagcggac
      901 cgttcagcac gtcgatccgc agtcccattt ccacctctac aacctgcacc caatgcgcgt
      961 cgttccagcg cggaaggact cttagtaaaa gcccagggtc tatcagtttt acaaaatatt
     1021 gtcgtccctt ttcctgcaaa aggcgtgcag agaggaaggg atgctgactc tgcaaaactt
     1081 tcagtgcatt tcggacgatt tgtggggaag gcgcaccctc tacgcggaga acactgatga
     1141 tatgaaacgg cgcatattgg tccgcgataa caagcgctcg ctcgaacgcc cctaactcac
     1201 gcttcattat ggattgaacc gtacaatttt gtgtacattt cctttgaatg gggtctttct
     1261 cgaatcggta ttataactgt tcactagcat tggcaatcca ttcttagggc ggattgaatt
     1321 caggcctatg caatcgacca ctgtaccagg ttcaagttga aatgagtttc tttggaagat
     1381 catcgccagg gcgatcttga gctgcatcat acctaaagag gtcccaatgc aataacgagg
     1441 acctgctgag aatggcataa actcatatgc tgatttgtga atggattgcc agcgttcggg
     1501 ttgaaattga tcaggctcag ggaacacctc aggaatgcga tgcgtaatgt agggactcat
     1561 aacaacccac gtgccttttg ggatggcatg ggaatccagc tgaaatggct gcattgcaag
     1621 gcgacctgtc catgtgccgg ctgggaaaag ccgcatcgtt tctttgattg ccccttcgag
     1681 ataaggcaag tcttccaact gctctggtgt cggtatattc ccccccaact tttcgtccaa
     1741 ttcatggagg aggactctta atttttcagg atgttgaatc aaaaggaaga tcgtccaata
     1801 taaggccatc ccattggggt tatatccgcc gcggaacatt gtagtggtat gcccaaacag
     1861 ttcacgttca ctcaaccgtt cgggatcttc ttcgtgcata cggatcatca cagaaaggat
     1921 gtcatccccc gtcaagcctt cttctttctt atgttggatc acatctttca cgatacgttc
     1981 catctcattt gccgtcctta gcagcttacg aaatggcatt ccagggatat cgtaaggaaa
     2041 caggagcaca aacggattca tgatcagatt catcacgcgt tcaagttgcc gcccgaacac
     2101 ttctccatat ctttcaggat caagcccaag cacaggactg gttgccagct tcatggcgag
     2161 ctgttccatt tcagtgcgca tgttcacttt cgcaccgatc ttccaggaag caagagtttt
     2221 ttcagtcgtg atcacacaac cctcatgata acgggtaata aatttcttat ggaaatatgg
     2281 gagaagtgca ctgcgatggt catggtgacg cccaccgttc atgagcggca tcccggttgt
     2341 caggcgagca agtgagcttt ctctcggaaa aggaacaggt accagttcca gatcgtagct
     2401 gtaaaaggca gaggggtcac gcaggacttg ccggttgtat tcgggatcaa acgcgatgac
     2461 tacaggatag ttcttaatct ttagcttgat cagattgcca tattgcctgc gaagatcctc
     2521 caatgtagag agaggcgaaa aagcaaactg gaatgcaaaa ggcaaccagc caaagatcgg
     2581 catgggtgat ggacctggta tctcatcgtt aaaatttaaa gacgttgctg ttgccatcct
     2641 accctccaaa atattcactt agtatcaaat taaaaattta ctcctgaagc ccagcctgct
     2701 taccggcttc ctgtgcaatc gttagctcat gcattgcacc catcttttca aagatccgaa
     2761 ttgcacgatc gaaatgttca cggcgcttgt gtagatcctc ctgagatgat gttcccaatt
     2821 tgacgcggat caacccttcc tgataaggca tgttgaattt cttcgcagcc tctacgcctt
     2881 tgttccaggt tttaatcgca gcctgtggtt tatgtgtcag ccactcatac caaccctgat
     2941 aataaagggt aacgggctgc ccaatcggga aaaccttctg gaacgcgacg agtagtttga
     3001 gggctttttc ggcaagcagt tcatatttat cagcttcaaa atgtctgttc gaatcctgaa
     3061 gcgctttttc ccataattca tagtatacgt ctgccaccgc ggcaaaccca atatccatgg
     3121 aatatactgt aggcgatatc cctgctgcca gttcaagaac tgcgctggca taggcaacgg
     3181 cctgtccgtg ttggcccagg cgcaaatggg ccagggccag ctgtccgttt gtgttgatgg
     3241 aagacgcaag gttgggaagc tcttcaagga tttgaagggc ctcctccagc atggggatag
     3301 cttctgttac gttccccaac cgaatactat tggcagccac accgaacaat ccccaaccct
     3361 gttgcaacgg attccgtctc ttatgcgcgt cttcaaggag tatcctttgt atgttcagcg
     3421 catattctat attgctggag ataagtgcat tttcggctaa tagaacagtt gcatcccccc
     3481 actggcggta atcgccaagt tgttcgcaaa ttgcctgggc ttccaatccc ttggctcgta
     3541 cgctatccca ttttccgatc ccaagttcgt acacacccgt gacaacgttc acggtgatca
     3601 gattggacgg ttggttaact gctttcgcga cttttagtgc ccgatccaca taggattgag
     3661 ccaatgtatg taattgggcg aatcctgaca gaacggccat acccgcgtaa cctgacgcca
     3721 gctcggcaga gggtccggct cgttcagctg tattcaacag acggagaata caatacatga
     3781 tcggaagcgt ctcattggaa taaaaataaa ttctgctcat taattcatag agtcgggaaa
     3841 cgtcaatcgc gacctggcgc tcctgctcat tggaaatgga gccaatatac cgggatggga
     3901 ataagcgatg aaatatctga cggataattt gaggaaccag ccctacagca aactgggcac
     3961 gcgaggctgg gatagggctt tccaacaatc ggagcgcctc gcggacatgc ttgttacagt
     4021 ctgtcaggga cccgagacta tagtgagcca gccctagctg gccatgccaa cgtgcctgac
     4081 gaatttgacg gcgataggat gcttgtttgt cttctggttt cggcagtttc gaatcccaat
     4141 ccaaagcctg tgtaaaaaac tggaccgctt ccttattggc ataggtttgc attgccttct
     4201 cccctgcctt ttcaagataa tccactgcct tatatacagc gtaatccttt tcagcagcct
     4261 ccgtaacttc agcagcctga gtccagtgat aagccagcaa ggcataataa gattcgatat
     4321 tcatctcatt gctttgttca atccattccg ccactgcctt atgcagttga cggcgctggg
     4381 aataaagcat gaggttatag gcaacctcct gagtgaccgc atgtttgaag atataggcca
     4441 actcgggtgt ttcagattca accaacgtca gactcaggcg cgtcaatgtc tccatataat
     4501 cctgaagagc cggcttatca gcttcgatgg gatgaaccgc ctccaggaca cgcagcgcaa
     4561 agatgcgccc aatcacgctg gcaaccttga gcgttagctg ttgcgaagga tcgagactat
     4621 cgatacggtt tgtgatcgct gcctggagcg tgtccggaag agtcaaatct tcaaaattgt
     4681 tcaggtctgg gtcaatctgg cattcctgat tttcaatttt caggataccc gcctcccgca
     4741 tcgcatacgc gagttcctcc gcaaagaagg gatgcccttc tgatttttca cggatcagcc
     4801 tgccgatcac gggcggaatg gatttgactc ccaaacgctg acacaccaac gcttcgacat
     4861 catccagcat catcgcttcc aaacggatca agcgtgcttg tgaagattcc tgaagttgct
     4921 tgaactgaag tgggacgggg tctgagatcg gtcgggtatt gagcgccagc aagatcggcc
     4981 gtactttctg ttgaacatct accaacaatg accaggaggc cgaatcaaac caatgcagat
     5041 cttccatcac gatcagcaaa ggtgcttgag cagtttcata agcaagcagg cgtgtcagta
     5101 cttcgcgaat gttgccgccg cgtatttcgc cagacatggc agaggtcaat tcattgtctg
     5161 ggagaggaat tggcaaaacg acactaagca agggtgcata acgcaccaga tcagcatcaa
     5221 tgctgtttaa agtcgccagg acttgatctt gaatcgcgcc cttatcttcc tctgtgagtt
     5281 cccctcctcc ggtaattgta tcgatatcga ggatacgatt aaaaatgggc cgccaggcat
     5341 ggtatggcgt ggttctctcg attgcatcgc ccgccccatt gagcatatgt acgtttaagg
     5401 tctcagcctg acgggcgaga tcctcgaata atcgtgactt tccaatcccc gcctctccct
     5461 gcaaaataat cgtctggtgc tctgtaccgc gcgccaattc ctgcagggca ttggcgatca
     5521 ttgtcttttc ttcctgacga ccgatcagtt cggtcttcgg gcgaatgaca cttttctttt
     5581 ggtccagcgg atgaaaaacc tctactgcgt ctgttcgacc ttttacatgc tgaggcaaaa
     5641 gcgcctcgaa ttccaccaca tctttcgcac tatcatacgt aagacgatcg cacagaatcg
     5701 ggacaccata cttactgatc aattcatctt gcatccccgc cgcacccatt agacgcgccg
     5761 agagattcac tgcatttccg atcgttgtat attcgcggcg ttcttcgttg ccgatcgatc
     5821 cgcaaaagat tcggccggtc gtgacaccaa tattgctacg aacttttcgg ctagtgagtt
     5881 tctttcgaat cagcaacgcg gcctgcacac cccgggttgg gtcatcttca tgagcaaagg
     5941 gcggcaaacc gaaggctgcc acaatcgtga tgcctttgtc gtctacattg attttgttga
     6001 tgctaccttc ataacgatag acagaactct gtattagttg tgcgatgctc tgggcggtca
     6061 tcagttccgt attctgatca agatccggca ggttgataaa gagcacggtt acgcgtctta
     6121 gttctgcgat ccaactgctt tgaccggccg aaagtcgatt gatgatcgca ccgggaatat
     6181 acgcccgcaa cgaacgttca gccccggctg gaatatccag ctttgtcttt gaagaaaaaa
     6241 tggaagacgg tttgttcaag ccagttaata gtccaccctg cgcgatggct tccttcagct
     6301 cgaattcaag tggcgctgca acgcagtcat ttcggatcag tttccatgag gatggagtaa
     6361 tgatgatctc acctgcatgt gcaaggttat tagcaatacc caattccaca agaggcgcac
     6421 ctgtgagcag aaattcccaa cgattaaact cgccccctac atgagcagtc gttatattac
     6481 cggtgctgat cgctaatttg aggtacaggt ttgagtcttc aactcgataa ttgttaagtt
     6541 gttcacggat ttttagagca cattccgcta cgcgcattgt ccattgccat tgatcagctc
     6601 tggagacaac attttcctta ctacctgagg tttgattaat attggcctgt tgtggtgaaa
     6661 ttggaagatc tgaaacaatc ggccagacgg caatcaatgc atcgcctgca aatttcacaa
     6721 tatctccccc atatgcgtgg ataatatcga tcaactgtcc gaaatactga ttcagaacgc
     6781 gagcaagcgt ctcaacccca gtcggacccg tttcggccaa acgctccgta agcggtgtaa
     6841 agcccgagat atcagcaaac agaatggcgg ctggtaattc ctccgtgacc ggcgactcaa
     6901 tcggccttgg gtcagcaaca attcgatttt gaataagttt tggcacgtag ctggcgagta
     6961 attccgtcag cgcggggttc gacatgcaag gctcctagat tcccaaacac gatcaaattt
     7021 cttaagccat gatacaccaa atcgtattta tttgaaatag ggttcacaat acaatggcct
     7081 aatttgaatt gtcgtccctc taaatctcta tagctcggaa tgctataatc cgttgaaagt
     7141 gttcatgaac agatcgttgt tatcgctact cgttcttttc ttctttacgt cctcctgtgg
     7201 ccctaaatct cagccctctc caggtgacac ctggacacaa cttaagtcga tgccaaccgc
     7261 acgttccgaa aatgccgccg ctgccgttgg cgacattatc tatgtttcgg gtgggtttgg
     7321 tggggaacag aagctggaag cctatgacac tacagagaac acctggcgca ctcttgccga
     7381 cctccctgag ccgcgccacc atttgatgtc cgcatcgcat aacggaaaag tgtatatttt
     7441 cggcggagcg gcctcaattg tcaattgggc tccccgcgcg gatgcctggg tgtacgaacc
     7501 agaaacagat tcctggagcc gtgttgccct catgccggaa cgacggctgg ctggtgcagc
     7561 ggtcacattg ggagattaca tttacgtgct aggaggtacg ggaggatcga atgcgctgtt
     7621 acgttacgat cctgcccaag atgaatggac taaactggcg caactatccc agtctcgtga
     7681 acataccacc gccacaacgt ttgacggaag actctataca ttgggcggac gctggtctgg
     7741 aatcggtgaa ttaacctctg ttgagattta tgacccagcg agtgactcgt ggacatctgc
     7801 gcaaactgca cgggcggggt tcgccacggt caccgtaaat ggaaaaatct atgtgatggg
     7861 aggtgaagtg ctcaccggcg cgaatcaggc attgaaaagt gttgaggttt ttgatctcgc
     7921 aagtggaaga tgggaatttg cgccggactt gcctttggca cttcatgggg ttccggctgt
     7981 taatgtcgat gatgtgatct atgtcttagg gggagcggat cgagctggtg caatttcgaa
     8041 tcagggtttg gtgttttctt ttacaccatg aatgacattc aacattggaa gcattataat
     8101 gactctgcca tttcagttcg gtgaaaacaa acaggagaat ccacatgcag tcattcaata
     8161 tcaatgatgc ggttagcttc aggctggcaa cccaacagga ggacaccggt gccaaaatgc
     8221 aggttttctt cggtgtgctt cttggtgtta ttcttcgttg gattgtggta gatgtcataa
     8281 agaatcaagt ccctcaaaat atacctgtga tcattgcacg tgtcgtagta gctttgatcg
     8341 tgaccatctt tgttttttcg gggtactggc aaaaggtgaa ggaacagccc cctggcatgc
     8401 gctttctgaa cgcgctggcg tatggggttt ccattgatgc tcttgtcggt ccctgggcat
     8461 tctaagagga gaatgacatg gactctttca gtttcaatga cgcattacgt ttcaggctgg
     8521 caacccaaca agaggacacc aatgccaaga tccaggtgtt catcggcgtt ttcctgggtg
     8581 taatcctgcg ttggatctat agtgttgcag tcgatgtgat tcgaaacggt ggctcgtggt
     8641 gttttggatc tctgactaac gtcttgatcc gccttgctgt tgccctgggc atcacgtttt
     8701 tcgttttttc agggtattgg caaaaagtga aggatcagcc tgccagtatg agattctgga
     8761 atgcagtagc gtacggtatt tccattgatg tcatcgtgag ctcgtggctg ccttcgggtc
     8821 cgtgttagct aagtcataaa tattttagaa attaaagcaa aagagagaag gttttaagcc
     8881 ttctctcttt tatttatact tgatcctact cactcgtttc actgcttttt gtttcactgc
     8941 tcttggactc gctggatttg ctttcttcct tcttttcgga tttcccttct ttcgatttgc
     9001 tggtggagga agaattattt ccggaggatg gcttgtcccc tgaaggggac ttgttgtcag
     9061 tggagtagaa ccccgacccc ttaaagataa ctttggaggg agtgatgact ttctttagcg
     9121 ttttcttgcg gcacgacgga cagatcttca acggttcatc gtgaaaaaat tgatggcgtt
     9181 caaattggtg accgcaggat gcacaacgat atacataaac gggcattaac ttctccttgc
     9241 attcaaaata gcgtgaggat tatagccccc ataacaatcg aaggcaaggg acactccgta
     9301 tgaagtgcgt taacgcatgc cgctgtcgta gttcacaatg atctgcgtga aggcaccagc
     9361 ctcaactcga aaggtctgtt ctccagcgta cggaatcccg tttagagact cggggtgcag
     9421 gatatattgg cccggcatga gcggaatctt gaagtgaccc tgttcatccg tctggacctg
     9481 cacgatcttt ttgccgctca aactgttgac cgttagcgtg gcctgatatg gctggtcggg
     9541 acattcctgc ccctcctgca ccaccgggca catcggcccg atcaatactt gtccttcaat
     9601 gccactgtcg gcgggagtgg catcaatgga gcaggtcgaa agaatcaaaa tcattaaacc
     9661 aataccaaac aataattttg tatccatggc tctctccttc tttctatact atcaactttc
     9721 agagagccag atttgttccc atgatcgttt taagttgttc tagccactag ggacggctga
     9781 gtttacagga acataatatt gcttatggca cgattgtaat tgcgggcaaa atactaaggt
     9841 cttcgcgcca cggaaggaga agggcaaacg attcgagagt ttgagtcccg ctgaccgtcc
     9901 ttctatacaa ataaaataga ttcctgctcc cctgaagcga ctcattcccc agtcctacag
     9961 ctccgatata accagcaaaa tgagcataat gaacagagtc ggtgttgtag gctccaaagg
    10021 gataggcaaa agtcaaaata ggcacgccaa gcatagcctg gagctttatg cgtgagtcga
    10081 cgatctcatc cttttgacgg tcggtgcgga ccgtcagatc gacgtgactg acgctatgac
    10141 tgccgatctc ccagcctgcg gcatatagcg tgcgaatctg ctcggcattc atgtaaccgc
    10201 caataccgat gtagttatga acaacataag ctgttccggt aaacccatat ttttgaagta
    10261 tgggcaatgc gttgataaag gtgccctcac cgccatcgtc gaaggtcagg atgattggtt
    10321 tgggaggcag ttctgcaccc tgattgatgg ctctgacgag caactccact gaaatcgtct
    10381 ggtaacccca ttcacgcaga aggaacattt gccgttcaaa ctcatagggc ggaacgtaat
    10441 aaaggctctc gcttttcgga ataccgatgt gatgataaag aaggatcgga acagtgactt
    10501 cgtttggacc ccgcatcacc cactgcggtg caagtgtagc tgtaggcacg ggtgtgaggg
    10561 tctcggtggg agtcgaagtt ggcgagatga aaacggtggc tgt
//
LOCUS       DASTJP010000420         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_215132, whole genome shotgun sequence.
ACCESSION   DASTJP010000420 DASTJP010000000
VERSION     DASTJP010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215132"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFQ23_12130"
     CDS             complement(<1..679)
                     /locus_tag="VFQ23_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9907390.1"
                     /translation="MVGKKILVLDENATSRNFLANTLREKQFKVFEAASGKEALILAW
                     RDEPDLVLFDPVLSDIKEREFIQKLRSDSRTSTFPLIALSSDPSSNRKEACLSSGVNE
                     YFIKSSQALSALDESFSRLFVEPQTSDVPGKNPGLLIVFLSAKGGTGTSSLCANLAMN
                     IKQSQPEASVVVADLVLPIGSIGQIVGYDDRPNLVSVADLTAAQTNIAYFRNNLPEPE
                     LWQFQLLA"
     gene            complement(695..1390)
                     /locus_tag="VFQ23_12135"
     CDS             complement(695..1390)
                     /locus_tag="VFQ23_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9907391.1"
                     /translation="MAKRILIVDDEPRYLRLLEANLRTEGYEVVTAQDGMQAVSVFSD
                     QPIDLVLMDVMMPKLDGFGATQRIREFSSIPIIILTAKGDEQDRVRGLDLGADDYLVK
                     PFSATELLARVRAVLRRAQPPSEVGQSRFFTHDNLKIDFARAEVWRGETPVSLSATEY
                     RLLLQFAHHVGKIMTSEDLLTSVWGPEYRTDKEILWVSIARLRQKLEDDAHTPKHIVT
                     RSGLGYLMPPIED"
     gene            complement(1409..>2365)
                     /locus_tag="VFQ23_12140"
     CDS             complement(1409..>2365)
                     /locus_tag="VFQ23_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9907392.1"
                     /translation="TFAGQVLKSGQLLLQDPKSDAPLDDRLSRAYLLGLPLRSDIAIK
                     GALIFVRFGGPVYESEHITLASFAADLISNLFERAAWKETISELSELKRQMQLQEDFV
                     STISHELRTPLGFIKGYSTSLLRPDTSWDAETQKEFLTIIDEEADRLSLLIENVLESA
                     RLQSKTLPLRFQPLRLDSVLRDVIMRLRTRHKDLDVSMDLSPAPPILGDGVRLSQVFE
                     NLFTNAIKYAPGAPIVITLNQEGESILISFKDHGPGIPTESLPLIFERFYRVRIEKTV
                     TGTGLGLYICKQIIRAHRGKIWAESTLGQGTTFFIELPINSS"
BASE COUNT          617 a          588 c          576 g          584 t
ORIGIN      
        1 ctgccagcaa ctggaattgc cataactctg gctccggcaa attattacga aagtaagcta
       61 tattcgtttg tgcggcagtg agatctgcca ccgagaccag atttggccta tcatcatagc
      121 caacgatctg accgatggag ccaatcggca aaaccagatc agcaaccacg acgctggcct
      181 ccggttggct ctgcttgata ttcatcgcaa gattggcaca taatgacgac gttcctgtgc
      241 cacctttcgc gctgagaaag acgatcaata gtccggggtt tttacccgga acatcagagg
      301 tttgtggctc aacgaacaag cgcgaaaaac tttcatccag cgcggaaagg gcttgggagg
      361 atttgatgaa atattcgttc acgcctgaac ttaagcaggc ttcttttcgg ttggaagagg
      421 gatcgctgga aagggcaatg aggggaaacg tactggtgcg cgaatcgctc cgcagttttt
      481 gaataaattc ccgctcttta atatcagata ataccgggtc aaataacacc aggtcaggtt
      541 catctcgcca ggcgaggatg agcgcttctt ttcccgaagc ggcttcaaaa accttgaatt
      601 gtttttcgcg aagagtattg gcaagaaaat tacggctggt agcgttctca tcaagaacga
      661 ggattttctt accaaccata aaacatccaa aagctcaatc ttcgatgggg ggcatcaaat
      721 atcccagccc cgagcgcgtc acaatatgtt ttggtgtatg agcgtcgtct tccagcttct
      781 ggcgcagacg cgcaatgctc acccataaaa tttccttatc cgtgcgatac tcaggacccc
      841 atacactcgt cagaagatct tccgaggtca taatcttgcc gacgtggtga gcaaattgta
      901 aaagcaaacg atattctgtg gcggaaagtg aaaccggggt ttccccgcgc catacctcgg
      961 cgcgtgcgaa atcgatcttt agattgtcat gcgtaaagaa gcgggactgt cccacttcac
     1021 tgggaggctg tgccctacgc aacacggccc ggactcgcgc taataattct gtcgctgaaa
     1081 aaggtttgac cagataatca tcggccccca ggtcaagacc acgcacacga tcctgctcat
     1141 ctcctttcgc cgtgagaata atgatcggta tgctggagaa ttcacggatg cgctgcgttg
     1201 ccccgaagcc atccagcttt ggcatcatca catccatcag aacaaggtcg atgggttggt
     1261 ctgaaaaaac gcttaccgcc tgcataccat cctgcgctgt gacaacttca taaccttcag
     1321 tgcgtagatt ggcttcgagc aatcgtagat aacggggctc atcatctact ataagaatgc
     1381 gttttgccat gtggcctcct tcgcgttatt atgatgaatt gatgggcagt tcaataaaaa
     1441 atgtagttcc ctggccaagc gtggattctg cccaaatctt accacgatgt gccctaatta
     1501 tctgcttgca aatgtagagt ccaagcccgg tgcccgttac ggtcttttca atgcggacgc
     1561 gatagaaacg ttcaaaaata aggggcaacg attctgttgg aattccaggt ccatgatcct
     1621 taaatgaaat aaggatgctc tctccctcct gattcaatgt gataacgatg ggtgcgccgg
     1681 gcgcatactt aatcgcattc gtaaacagat tttcaaaaac ttgtgatagt cgaacgccat
     1741 cgcccagaat cggaggcgca ggagatagat ccatacttac atccagatcc ttgtgccgag
     1801 tgcggagacg catgatcacg tcgcgcaaca cagaatcaag cctgaggggt tggaagcgaa
     1861 gcggtagagt cttgctttgc aatcgcgcgg actcaagcac attctcgatc aataacgaga
     1921 gccggtcggc ttcttcatca atgatcgtca ggaattcctt ttgggtttct gcatcccaac
     1981 tggtatcggg ccttaagaga ctcgtgctat agcctttgat gaaacccagt ggtgtgcgca
     2041 attcgtgtga gatcgtgctg acaaaatctt cctgcagttg catctggcgt tttagttcgc
     2101 ttaattctga aatggtttct ttccatgccg cccgctcgaa caagtttgaa atcagatccg
     2161 cggcaaagga agccagtgta atatgttcac tttcataaac gggaccgcca aagcgtacaa
     2221 aaatcagcgc acctttgatg gctatgtctg agcgaagagg caaacccagc agataggcac
     2281 ggcttaaacg gtcatccagc ggcgcatctg actttggatc ttgaagcagt aactgaccgc
     2341 ttttgagtac ctgaccggcg aatgt
//
LOCUS       DASTJP010000421        17873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_248565, whole genome shotgun sequence.
ACCESSION   DASTJP010000421 DASTJP010000000
VERSION     DASTJP010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17873
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248565"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFQ23_12145"
     CDS             <1..596
                     /locus_tag="VFQ23_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907393.1"
                     /translation="ADGLSAATDHRIDFVNLTTNGGTPESLLKSIQTSEKTRAAVIAA
                     DIIIISTGMNDLEPTIDLYSAGKCGGADNFNCFRQVAEGWRLSFNEILTEINNLRNGK
                     PTAIRLVTNSNEFLSDRELMQLFGGPEFGPKGGALITNLHHDVLCEVAAKHGAVCVDL
                     RPVLNGPNLDKPQDANTQEAMQAVAGALLASGLDELR"
     gene            727..1620
                     /locus_tag="VFQ23_12150"
     CDS             727..1620
                     /locus_tag="VFQ23_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9907394.1"
                     /translation="MFTNRLFCLSIVIAILVVAACTPQVEVTPTATSLPETNTPQVEM
                     TATEPPSPTLTVTSEPHWNLVVVGDSIPYNSPNDCPGCTSFVDRYAEAITVATGKAVK
                     VQNLSTHTGLQIDGLLQMLASSKMQESIANADIIIVGIAHNDAAMNSNDDLCDGPNGD
                     NPDWSKYNAECAIASAELFQPKFESVYSQIVALRDGKPTIFRTINRYNDWNGWPGHDL
                     SPEGIKATKEVIDAWNEMICKAAEDNGFVCADIYSEFNGSDGLTPSGDLVARDYTHPS
                     DKGNEVIAQVLTEIGFAPLVP"
     gene            complement(1892..4471)
                     /locus_tag="VFQ23_12155"
     CDS             complement(1892..4471)
                     /locus_tag="VFQ23_12155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9907395.1"
                     /translation="MLSLRTLLRHPIQFAIMLLGIALGVAVMVSIDLANASAERAFDL
                     SMTAVTGRATHAIVGSNQGVDESVYVSLRTDPQWRTAVESAPLVIAYAVSPQLGDIPF
                     TVLGIDPFAEAPFRGYLGNGEGTRPETIAALMMSPNSVVLSASTAQRYGLQPCQRSED
                     PYVVSPTDACRITLSINGEPREVYLTGLLEPSDDFARRALETLILTDIATAQSLSNTN
                     GHLTQIDLILPTEFDIASLSAVLPPGTLLIPSELRNDQVNEMTSAFRFNLTALSLLAL
                     LVGIFLIYNAMTFSVVQRRAVFGTLRSIGYTREQVFGMVVGEAALVGALGSGLGLALG
                     ILLGQGAVQMVSQTINDLYFAVSVRGVQIPVSSLLKGGVAGLTASIFAAAAPAWEAAS
                     IAPRLALSRVGLESKIESIVNRVAQIGVLIALVGVLILVIPTRDLVISFSGTFAVVMG
                     LAALTPFVTITVMRLLSKPLARFFGLVGRLAPRNVIRSQSRTAVAVAALMIAVAVTIG
                     VQVMISSFRTTVQIWLEQTMRGDIYISTQGLNATRLDTPLDPQIISLAQEYPEADSTI
                     AIRVVTAESDHGPVELLAVHAQRPMDPRLFLASQGNPEQTQQMMEEGAILLSEPLANR
                     LGISSADGSLALLTPNGWQSFPIAGIYSDYTSTRGTVRMDLDVYRRLWNDNDLNGVAL
                     ILEPNANVDEVSADLRTRLTDFPNIQVNPSSALREEAMVVFDRTFAITTAMQLITTLV
                     AFIGVLSSLLAMQIEKAREIGILRAIGLTLAEMRRLTLWETGLLGASAGLLALPTGYI
                     LAWILIFVINQRSFGWTLQMHLELAPFMQAFLLSVSAALLAGLYPAWRLSHMQVAEAL
                     RGE"
     gene            complement(4473..5153)
                     /locus_tag="VFQ23_12160"
     CDS             complement(4473..5153)
                     /locus_tag="VFQ23_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907396.1"
                     /translation="MSSFLRFHDLTKSYYEGDVQRIVLQNAHAEFQAGEITAVLGKSG
                     SGKSTLLNLISGIDAPDSGQVWVDGQDLTAMSERDRTLFRRARIGFVFQFFNLIPTLT
                     VGENVSLPLELNHVPRAQAHKKAQDFLETVGLQDRWKTYPEKLSGGEQQRVALVRALI
                     HDPVLILADEPTGNLDEETGKQVITLLARLTREQNRTLLIVTHSPEVTLLADRVMRLS
                     HGQLVEER"
     gene            5233..5394
                     /locus_tag="VFQ23_12165"
     CDS             5233..5394
                     /locus_tag="VFQ23_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907397.1"
                     /translation="MLFSSLRIPFTFLLISILLASCAPASVPSAELQVPTVVAINGNS
                     SPDFAAAND"
     gene            complement(5668..8706)
                     /locus_tag="VFQ23_12170"
     CDS             complement(5668..8706)
                     /locus_tag="VFQ23_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9907398.1"
                     /translation="MNRLVSLLNVRADEGRLVALVAILFACIQAGQGMGDNAATALFL
                     LRYGVDFLPYMYLFLGALTFAATLAYSAALGRFEKGRFFSTLVIGFIGLLLFERAAIL
                     SKLAILYPILWLTINGAGMILGTFVWNIAGEVCDARQAKRLFPLFTSAGILGSVVGNA
                     VTGVVARLLGTDNLLILYAGLLFVAFYLTRTIALTYFRKEKISKTKSNFWNDLRAGFD
                     YVRASSLLRLIAYASILFSVLFFAVSFPFSEVVTKSFSDEAGVAGFFGLFNSITTAVT
                     FFVSLFLASRIYTKLGIINGVFLMPLTYIFAFAVFALFYNLNGASTARFAQQVILSGI
                     AGTAWNALFNVVPSQKRGQVLAFNNGVPSQIGVVLSGVLLIIAKRSLTTQQIFLMGTV
                     LAILCGVLIWRMRAAYAQALVDALRAGRVEVFSKNETSFSGLQGDAAAFHVAIRALDD
                     SKASTRRLAAEMLGRMELDSAIPHLTRLLADPEPGVRASAVSSLGVLFADSAIDEILS
                     LLNDEDEQVRQEVLRAIGRLHIAANAEQLKQIRQLAENDPALEVQTKAQIALFKLGET
                     ETALSSLIARLKSDDQQIRISALGAFAEILSALKNSVDINPILNALKDSSANVRRAAV
                     MALGKIKDESIIKTLVDSLFDPDPGVRNAAAEVLRNRSGQSRAYVLEIIKSKNGSIDS
                     ALDALAPGNPESLFPLREYARRELLHAKTLRSQSASIPASGKISKFLSDCLKMQSGLC
                     EGRLIKTVGLFGDMHTMELVRKSLRGTNMENRAAALEALDTIGDKQLAKSVVSLLEEE
                     PTRSDPSDVIALLLKDEDPWLRTLAIRSTSELGLSEFIPLLHQLKSGSDVFMREAALG
                     ALIQFGEEQPMSTLQTVSIFERILLLREIPIFAELSPEDLKLVADIAREEWYPQNTDI
                     FHQGDDGSAMFVIVEGRLQVLVSANGTERELAQRRPGEFVGEMAIIESVPRLATLRAQ
                     TDVRLLSIDGDTFKQILQERSDVSFAVLRSLSRRLREMSS"
     gene            complement(8709..9122)
                     /locus_tag="VFQ23_12175"
     CDS             complement(8709..9122)
                     /locus_tag="VFQ23_12175"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9907399.1"
                     /translation="MSLEKNKNIVRRYQEIYNSNHLDSLTEVVSEDLRTPKIMPGIPS
                     GIEGAKVAHQIMVTGFPDYQTVIDDLFAEGDKVVARITMSGTHLGNFMGMPATGKHVE
                     FTGIYIARIANGKIVEHWGEEDGVSLLSQLGVLKM"
     gene            complement(9192..12725)
                     /locus_tag="VFQ23_12180"
     CDS             complement(9192..12725)
                     /locus_tag="VFQ23_12180"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9907400.1"
                     /translation="MNCGTSLAQGAGARDGSRLIPKEPVATGEAKSTNFQLDRYLPKE
                     LVTKLESARSRNEMVGERRVVTMLFCDVKGSTAAAEKVDPEIWSEIMNGVFEFMIRPI
                     YKYEGLVPRLMGDAILAFFGAPITHEDDPQRAVLAGLEIQEGIKAYAEEIRLKYGLAF
                     GLRVGINTGLVVVGEIGSDLRMEYTALGDAINLAARMEQTAQVGTIQISDETYKLVAP
                     FFDVEALGEIEVKGKSAPIKTYRVLGAKSTPGNLRGLEGLSSPLVGRDAQLTALKDRL
                     KDLDRGRGSVVAVVGEAGLGKSTLIAELKKSDEGARFIWLRGDSLSYTSSISYYPWRQ
                     IIRQSVEAHENDSPAEVRHKLGYVCECCTLPGGDIPFLEAMLAVESEESAKVVMGFQG
                     DALKQRMIDATRGFMCGLSVESPLVIVFDDLHWTDEASLNLLLNVADLNAEQPILFIC
                     MSRPDKTAASWDSIQKMREKLGDAFHSIDLAPLQAEQTETLLNNLLDANELPISLRNL
                     IISKGEGNPFFIEEIIRSLIETKQIIREENHWKAMVDEAKISLPNTLRGVLSARVDRL
                     AETPKHVLQNAAVIGRLFDLRVLRRLTDLSGGLDPQIRYLQDVSLIEALLGEYAFRHV
                     LIQEATYESILLKKRAELHRQIGEALEEIHANRIEEFAPLIAHHFYRAGDDRSLKYDL
                     ISGEKSARLYANAEAATHFRRALEVAKRTTADLKQIAHLYTQLGTVLELSGKYQQALA
                     NHDEMLSLAIERHERGMELSALMAKATVYSTFTEVHNPELSEKTLIRALEVSQALGDI
                     SAQARLNWNLMLTYLFSKRLNQALPYGEAALDLARKSGNTEYLAFVLNDLCRLHTCRG
                     EFEKAHTFVRQARELWISLDHQVMLADSWGSEAEAYFNGGEFELGIECCEKALKISEE
                     INNLWGQAYNMMLMSFVYFENGELGRGIQLAERSVQLADQAGLIASSISLRSELGWAY
                     AYCGAFEKGLSLTIQPLQIADARQPAWRSFPLASRARMYIMKGDLELAGETIGDKPLQ
                     PTTIPYARYTIFVCLANLEFAYAKGKYAEVLKLADELMAEVKSLTRVDVPDVLRLKGL
                     ALLGLNRLDEALQILTEACSLAKEIDANLQLWSSLAGLADVQSKLGKQKEAEDSLVEA
                     RKIVAQVAESLREIGLNESFLNQPQVRKLMR"
     gene            12894..14276
                     /locus_tag="VFQ23_12185"
     CDS             12894..14276
                     /locus_tag="VFQ23_12185"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET9907401.1"
                     /translation="MAKYRIMYGKHIPQSCTVEGNERTIKKQLSQKIQGKIYAYSMAE
                     GLTSTSDYAAQYKRGAVRFNNEKTQLTYNDKMKKSAFYFCIVWLEILTACAPATVPAV
                     ELQVPAVTALNGNPSPDFAAADGSRRLAFPKDFGPHEEFRTEWWYYTGNLQTSEGRHF
                     GFELTIFRVSLLPPTIELPSESQWYDHSLYFAHFAVSDIANEKFYAFERYSRPGPGLA
                     GAQVEPYRVWIEDWNIIEQVPGIYHLQAAQTDIAIDLTLTDEMGVVLHGESGYSRKGE
                     DVTSASYYYSQPRLRAEGFLEVDEVRYSVTGLVWKDHEFSTSVLDENQIGWDWFSLQF
                     EDGKALMLFQLRERDGGTSASSSGTLIDMNGTPQHLQKTNFEIIVRDQWQSPHTQGVY
                     PSAWEVNLKDLGCRLEIHPWMADQELHFPTVTYWEGAVRFEGTCENGESVRGNGYVEL
                     TGYSGNVPLP"
     gene            complement(14365..15501)
                     /locus_tag="VFQ23_12190"
     CDS             complement(14365..15501)
                     /locus_tag="VFQ23_12190"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET9907402.1"
                     /translation="MKRISSLFLPGVVAVVLLAAACAPVEEQTLTPAAQAETSSPIAV
                     VTDTSATPLATATDTTLTPSIPVTGLELIQLDCQYCVDTIAHAQLVIPREASFRVTSP
                     PELANDDDDDGDGDGGHRRTAQLSGAAEVPGPGDPDGSGTAMIRLSLEADEMCFDLTA
                     SNIGPATAAHIHEGAEGVAGEVVVPLDPAPAEGSSSGCLSDVDNALLQNIIENPEQYY
                     VNVHNEEFPDGAIRGQLARVDNDEDDDDNEDRLGCRVIDRFRDKKLVLCRAEENTTIT
                     MNVCMDENNCTEVQLTLQACALVTGTPTVTSTLVTTATASTTTLTPIVITATATSLTS
                     TATSTLIVPPTGSATPTSSLTNTPVASPTSTATSTFTPTSTPTP"
     gene            complement(15862..16599)
                     /locus_tag="VFQ23_12195"
     CDS             complement(15862..16599)
                     /locus_tag="VFQ23_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907403.1"
                     /translation="MLVNELLSYLFDGKPNPLTGPMTSWLSSSRRFTTFVNAHHTKIR
                     KKIRTVQGSENLRDLQLELETAYLLVREPTLGVAYEPQHPDLGRSPDFAVAFTTSLTF
                     MVEVTRLRSIGTRTSPGAEKQADGLPWMDRFTDMLCSKLGQLLSKRSNLLLVGLEAYH
                     LTQSDLHAIMLSIQKRAETNDPNVLQRHGFRDRADFFNHYQRLSAILIREIPLESEKP
                     VVLWNNPQGKYPLPSKVHTVLSRSHTI"
     gene            16955..17650
                     /locus_tag="VFQ23_12200"
     CDS             16955..17650
                     /locus_tag="VFQ23_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9907404.1"
                     /translation="MNLQAMPLDDLAQRCAQETILYFKSRIHDTSYCFELFRRAVTER
                     SELAWSAILAQYKPLVARWVNRQADKHPDFPLAREEEQDFVDEAFIRFWHYFTSEKFH
                     NSKGLDGILKYLKMCVNSAVLDILRKAQHVQFEQKPEGEEEDDETNQGESGPTPEESL
                     QSDEVWKAIKARLKDEKEHVVIYASFFLGLSPREIFMEYPDVFRSIDEIYQCKANVLA
                     RLQRDSELRDLFK"
BASE COUNT         4614 a         4492 c         4451 g         4316 t
ORIGIN      
        1 atgcggacgg cttgtcagcc gcgacagatc accgcatcga cttcgttaat ctcacgacga
       61 acggcggaac accggagtca ctgctcaaga gtatccagac atcggagaag acccgcgctg
      121 cggtgatagc tgccgacatc ataataatct cgactgggat gaacgacctc gaaccgacaa
      181 tcgatctcta ttcagcagga aaatgtggcg gggctgacaa tttcaattgt ttccggcagg
      241 tcgctgaagg ttggcgttta tctttcaatg aaatcctcac tgagataaac aatcttcgca
      301 atgggaagcc aaccgcgatc cgacttgtta cgaactctaa cgagtttcta tctgaccgtg
      361 agctaatgca actcttcggg gggcctgaat ttggaccgaa gggaggcgct ctcattacca
      421 acctgcatca cgatgtcctg tgcgaggtcg ccgccaagca tggggctgta tgtgttgatc
      481 ttcggccagt cctcaatgga ccaaatctcg acaaacccca ggatgcgaac acacaagaag
      541 caatgcaagc cgtagcaggc gctcttcttg caagcggtct cgacgaactc cgctaactat
      601 tgtagttcat ggaagtcgct aatcggtttt cagctacttt atagtattaa gtcggtaaag
      661 cctcgcggat gatcactttg taaggtgttg ctgttatatg aagtatatta tctgaaggag
      721 atgaaaatgt ttaccaatcg tttgttctgt ctgtcaattg tgattgcaat tcttgtagtc
      781 gcggcctgta caccacaggt tgaagtaacg ccaacagcaa cttccctgcc ggagacaaac
      841 acaccgcaag ttgaaatgac ggcaacagaa ccgccctccc caactttaac agtgacgtca
      901 gagccgcatt ggaatctagt agtagttggc gactcgatcc cttacaacag tcccaatgac
      961 tgccccggct gcaccagctt cgttgaccga tatgctgaag cgatcacagt tgccacaggt
     1021 aaggctgtga aggttcaaaa tctctccaca catactggac ttcagatcga cggtcttctt
     1081 caaatgttgg caagctccaa aatgcaggag tccattgcca atgcagatat catcattgtg
     1141 ggaatcgccc acaatgatgc tgctatgaac agtaatgacg atttgtgtga tggaccaaac
     1201 ggagacaatc ctgactggtc gaaatacaat gcagagtgcg ccattgcttc cgccgagctt
     1261 ttccaaccca agtttgaaag cgtttattct cagatcgtgg cgttgcgaga cggtaaacct
     1321 acaatctttc gaaccatcaa tcggtacaac gactggaatg gttggcccgg acatgacctg
     1381 tcacccgaag gcataaaggc aacaaaagaa gtgattgatg catggaacga gatgatctgt
     1441 aaggcggcgg aggacaatgg tttcgtctgt gcagatatct acagtgagtt caatggatct
     1501 gatggactca cgccttcagg agatttggtg gcaagggatt acactcaccc ttcagataaa
     1561 ggcaacgaag tgatcgcgca ggttctaaca gagattggtt ttgctccact agtaccctag
     1621 ttgagttttt caaatcatag taacagcctc gcggacgaaa tgttcgcgag gctgttggtt
     1681 atatgagtag tgtggctgat cttctccctc ttttatatcg ctagcctgaa gaaggcacct
     1741 cagaagtgac aaacttgccc caaaacaatc catgtcgtga gtttaatcga gttaccccgt
     1801 tgtacattta gtgaaggcga gattcgcgaa ggcttggtcg gtagtcacaa cggcatgata
     1861 ccagcaccat aaaaaaagtt aatgagacaa tttattcccc acgcaaagct tctgcaactt
     1921 gcatatgact caatcgccag gcggggtaaa ggccggcgag gagggcggcg ctgactgata
     1981 gtaaaaaggc ttgcataaag ggcgccagtt caagatgcat ttgcagggtc cagccaaacg
     2041 aacgctggtt gataacgaag atcaggatcc atgccaggat gtatcctgtg ggaagtgcca
     2101 gcagtccggc ggatgcccca agtagaccgg tctcccaaag cgtgaggcgg cgcatttcag
     2161 cgagggtcag cccgatcgct cgcaggattc cgatttcgcg tgctttctca atttgcatgg
     2221 cgagcagaga acttagcaca ccgataaatg ccaccagggt tgtaatcaat tgcatcgctg
     2281 tagtgattgc gaaggtgcgg tcgaatacca ccatcgcctc ttcacgaagt gcgctgctgg
     2341 ggttgacttg tatattggga aaatctgtca gccttgtacg cagatcagcg ctaacctcat
     2401 ctacgtttgc atttggctct agaataagtg cgacaccgtt caggtcgtta tcattccata
     2461 agcgacggta aacgtccagg tccatacgaa ccgtgccgcg cgtggatgtg tagtcagaat
     2521 aaatcccggc gatcgggaac gactgccatc cgtttggggt gagaagagca agcgacccat
     2581 ccgcagaaga aatgccaagc cgatttgcca gcggttcaga caacaaaatt gcaccctctt
     2641 ccatcatttg ctgggtctgc tcagggtttc cctgtgaagc gagaaacagc cgcggatcca
     2701 tcggacgttg cgcgtgaacc gccagaagtt ccactggacc atggtctgat tccgcagtga
     2761 caacgcgaat cgcaatcgtt gaatcagcct caggatattc ctgggcaaga gaaataattt
     2821 gaggatcaag cggtgtatca aggcgcgttg catttaaccc ctgcgtggaa atgtaaatat
     2881 ctccgcgcat cgtctgctcc agccagatct gaaccgtcgt gcggaagcta ctgatcatca
     2941 cctgtacgcc aatggtgaca gccacagcaa tcatcagcgc ggcgaccgca acagccgttc
     3001 ggctttgtga gcgaatgaca ttgcgcggcg caaggcgacc caccagtcca aagaaccggg
     3061 cgagaggttt actcaataga cgcatgacgg taatcgtcac gaaaggggtg agcgctgcca
     3121 gccccatgac taccgcgaag gtgccactga aactgatgac gagatcgcgc gtcgggatga
     3181 caagaatgag aacgccaacc agcgcgatta aaacgcctat ctgggctacc cggttgacga
     3241 ttgattcgat tttgctttct aatccaacac gagagagtgc cagccgcggg gcaatggacg
     3301 cagcttccca ggccggcgcg gcagcggcga agatactggc cgtgagacct gccactccgc
     3361 ctttaagcaa gcttgaaaca ggaatttgaa cgccgcgcac actgacggcg aaataaagat
     3421 cgttgatcgt ttgggacacc atctgcaccg cgccctggcc gagaagaatg ccaagtgcaa
     3481 gtccgagtcc agagcccaat gcacccacca gcgcggcttc gccaaccacc atgccaaaga
     3541 cttgttcgcg cgtgtagcca atacttcgca acgttccaaa cacagcgcgt cgctggacga
     3601 ctgaaaacgt catggcgttg taaatgagga aaatgccgac gagcaaagcc agaaggctga
     3661 gagccgtgag attgaaacga aaagcggatg tcatctcatt cacctgatca ttgcgcaatt
     3721 cggaagggat aagcaatgtg ccaggcggca gcaccgcgga gagtgaagca atgtcgaact
     3781 ctgtaggtag gatcaggtcg atttgagtca aatgtccgtt ggtattactg aggctttgag
     3841 cagtagcgat atctgtcaga atgagagttt caagtgcacg tcgggcaaag tcatctgaag
     3901 gttccagcaa gcccgtcaga taaacctcgc gcggctcccc attaatcgaa agcgttattc
     3961 ggcatgcatc cgtgggcgaa acaacatacg gatcctctga acgctggcag ggctgtaatc
     4021 cataacgttg ggcggttgat gcagaaagca ccaccgaatt gggtgacatc ataagcgcgg
     4081 ctatcgtttc agggcgtgtt ccttcgccgt tacccaggta accgcgaaat ggtgcttcag
     4141 caaagggatc gatacccaac accgtaaatg ggatatcacc taattgtggg ctgaccgcat
     4201 atgcaataac cagcggcgca ctctcgactg cggtccgcca ttgcggatct gttcgcaggc
     4261 tcacataaac ggactcgtcc acgccctggt tactgccgac gatggcatga gtcgcgcgtc
     4321 cggtcacggc ggtcatactg aggtcgaagg cgcgttctgc actggcattg gcaaggtcaa
     4381 tgctgaccat aactgctaca cccagagcga tgccaagaag catgatcgcg aactggatgg
     4441 gatgtcgaag caaagtgcgc agcgaaagca tgtcagcgct cctcgacaag ttgaccgtgt
     4501 gaaagtcgca tcacacggtc cgcaagcaag gtaacttccg ggctgtgcgt aacgatcaat
     4561 agtgtgcgat tctgttcccg tgtgagacga gcaagcaaag tgatcacctg ttttccggtt
     4621 tcttcatcca gattacctgt gggctcatct gccaggatta gaactggatc atgaatcaac
     4681 gctctcacca gcgcgacgcg ttgctgctca ccgccggaaa gtttttcggg ataggtcttc
     4741 cagcgatcct gcagcccaac agtttcaaga aaatcctggg cctttttgtg agcctgcgcg
     4801 cgagggacgt ggttaagctc caggggaagg ctgacatttt cacctacagt gagagtggga
     4861 attaagttaa agaattggaa gacaaagccg attcgggcgc gccgaaagag agttcgatca
     4921 cgttcagaca tggcagttag atcctgacca tccacccaaa cctgtccact gtcaggggcg
     4981 tcgatgccgc tgatcaggtt gagaagtgtg cttttaccac tgccgctctt gcccaaaaca
     5041 gccgtgatct caccggcttg gaattcggca tgcgcgtttt gcaaaacgat tcgctgtacg
     5101 tcgccttcgt aataagattt cgtcaggtca tggaaacgga gaaatgagct cataacggtg
     5161 attgtattcg gcgtgcatta gagaagtatc agtttgaatc gcacgtgcca taagtgaaca
     5221 tataatattg aagtgctctt ctcttccctt cgtattccgt tcacgttcct tctcatatcc
     5281 attttactgg cttcgtgtgc gccagcgtcg gttccatctg cggagctaca agtgcccact
     5341 gtcgtggcga tcaatggaaa ttcctcgcca gattttgccg ccgccaacga ttgaacttcc
     5401 ctctgattct cagtggtacg accgcagcct gtactttgct cactttgcag ttagcgatat
     5461 tgcgaatgag acaatgcgtt tgaacgttat tctcgtctgg gtcctggcct ggcaggcggg
     5521 caggctgaag cctatcgtgt ctggatcgag gattggaaca tccagtcgat ctcgaatgcg
     5581 gctatgcagg gggcgagtta tactcgattg attttcatgt aatccgtatt gtttcggttt
     5641 tttccgtgag agtggttcca gtggtgttta gctggacatt tctctcaagc ggcgtgagag
     5701 actgcgaagg acagcaaagg acacatctga gcgttcctgg agaatctgtt tgaaagtatc
     5761 gccatcaatc gacaacaaac gcacatcagt ttgagcgcgg agagtcgcca ggcgcggaac
     5821 ggattcgatg atcgccattt caccaacaaa ttcgccgggc ctgcgttggg caagttcacg
     5881 ttctgttcca ttcgcggaaa ccaatacctg gaggcgacct tctacgataa caaacatcgc
     5941 gctgccatca tcgccctgat ggaagatatc tgtgttttgg ggataccact cttcacgagc
     6001 gatatctgcc acaagcttca aatcttccgg tgaaagttca gcaaagatgg gaatctcccg
     6061 caacaataaa atacgttcaa aaatggagac ggtttgtaga gtgctcatcg gttgctcctc
     6121 accaaattgg atcaaggctc ccagcgctgc ttcgcgcata aagacatcag agcctgattt
     6181 cagttgatgc aataggggaa taaattcact caagccaagt tcggaagtag atcgaatcgc
     6241 cagagtccgc agccagggat cttcatcctt caatagcaag gcaatcacat cagacggatc
     6301 agatcgcgtg ggttcttctt cgagtaatga aaccacgctc ttcgccagtt gtttgtcgcc
     6361 gatcgtgtcc aatgcttcca gcgcggcagc gcgattttcc atatttgtac cacgcagact
     6421 cttacgcaca agttccatcg tgtgcatgtc accgaacaaa cccacggttt tgatcaatcg
     6481 cccttcgcaa agcccagact gcattttcaa gcagtcgctc aggaatttcg aaatttttcc
     6541 cgaagctggg atgctggcag attggcttct tagtgtcttt gcatggagga gttcacgtct
     6601 ggcgtattca cgcaaaggaa aaagtgactc aggatttccg ggtgccaacg cgtcgagggc
     6661 ggagtcaatc gatccgtttt tggatttgat gatttccaga acatatgcac ggctttggcc
     6721 gctgcgatta cgcaatactt ctgccgcggc attacgcaca cctgggtctg gatcaaacaa
     6781 agaatcaacc agtgtcttga ttattgattc atctttgatt ttgccaagtg ccataactgc
     6841 agcgcggcgg acattggcag aagaatcttt gagagcgttt agtattggat ttatgtcgac
     6901 tgaatttttt agtgcactca aaatctcagc aaaagccccc agtgcggaga tgcgaatttg
     6961 ttgatcatcc gatttcagac gagcaattaa gctcgataaa gcagtttcgg tttcaccgag
     7021 tttgaacagg gcaatctgtg cctttgtctg aacctcaagc gcaggatcat tttctgccag
     7081 ttgcctgatt tgttttagct gctcggcgtt tgcagcaata tgcaaacgcc caatggccct
     7141 caacacttcc tggcggactt gttcatcttc atcgttgagc agggaaagaa tttcatcgat
     7201 cgctgagtct gcgaataaaa cccccagcga agaaacggct gaggcgcgga caccaggctc
     7261 cgggtcggcg aggaggcgcg tgagatgagg gatggcagag tccaattcca tgcgaccgag
     7321 catttcagcg gcgagtcggc gcgtggaggc ttttgaatca tccaacgcac gaattgcaac
     7381 atggaaggcg gcagcatcgc cctgaagccc gctgaatgag gtttcattct tgctgaacac
     7441 ttccacacgg cccgcacgga gagcatccac gagggcttga gcatacgcgg cacgcatgcg
     7501 ccaaatcaga acgccacaga ggattgcgag gacggtgccc atcagaaaaa tttgttgggt
     7561 agtcagcgat cgtttagcga tgatcaacaa aacaccggaa agcaccacac caatctgcga
     7621 ggggacgccg ttattgaaag cgagcacctg tccgcgtttt tgcgaaggga cgacgttgaa
     7681 gagcgcgttc catgccgtgc ccgcgatgcc tgagagaatc acctgctgtg caaaccgagc
     7741 agtggaggca ccgttcaggt tataaaaaag ggcgaagacc gcaaacgcaa aaatataggt
     7801 gagcggcatg aggaacacgc cgttgatgat gccgagtttg gtgtagatcc gactggcaag
     7861 aaacagcgaa acaaaaaatg taacggcggt cgtgatgctg ttgaacaaac cgaagaagcc
     7921 agcaacaccc gcttcatcgg aaaacgattt ggtgactact tcgctgaacg gaaatgaaac
     7981 tgcgaagaac aacactgaaa ataaaatgga ggcatatgct atcaatcgca ggagtgatga
     8041 agcgcgcaca taatcgaagc cagcacgcaa gtcattccag aagttggact ttgttttgga
     8101 aattttttcc ttgcggaagt atgtgagtgc gatcgtgcgc gtcagataga acgcaacaaa
     8161 caacaaaccc gcatacagaa tgagaagatt gtctgttccg agcaggcggg caacaacacc
     8221 tgtaacagcg ttgcccacaa cactgccgag aatccctgca ctggtgaaaa gcggaaagag
     8281 acgtttcgcc tgccgtgcat cgcagacttc gcccgcgata ttccacacga atgtgccgag
     8341 gatcatccct gcaccattga tggtcaacca taaaatggga tatagaatcg caagcttcga
     8401 taggattgcg gcgcgttcaa aaagtaaaag cccaataaag ccgataacca gagtggaaaa
     8461 gaatctgcct ttttcaaagc gccccagcgc tgctgagtag gcaagcgtgg cagcaaacgt
     8521 tagagcccct aaaaacaaat acatgtatgg cagaaagtcc acgccgtagc gcaacaaaaa
     8581 caacgccgtc gccgcgttat cgcccatgcc ctgccccgct tgaatgcacg caaacaggat
     8641 ggcgaccagc gcgaccagtc gtccttcatc agcgcggacg tttaataacg aaacaagtcg
     8701 attcatattt acattttcaa aacgccaagc tgactcaaca gactaacgcc gtcctcttcg
     8761 ccccaatgtt ccacaatctt gccatttgcg atgcgagcta tgtaaatccc ggtaaactca
     8821 acatgtttcc cagtcgcagg catgcccata aaattcccta agtgagtgcc actcatggta
     8881 atacgcgcca caactttatc gccttccgcg aacaaatcat cgatgactgt ttgataatcg
     8941 ggaaaccccg tcaccatgat ttgatgcgca actttggctc cttcaattcc ggagggaatc
     9001 ccaggcatga ttttgggagt acgcaaatct tcagacacca cttcagtcaa cgaatccaga
     9061 tgattactgt tgtaaatttc ctgataacgg cggacgatgt ttttattttt ttcaagggac
     9121 ataacgcttc tcctctttat catgttagca agtgtcgcgc ggcgccacgg cgaccctggc
     9181 tgactccaga tttagcgcat caatttccgg acttgaggct gattcaaaaa cgattcattg
     9241 agtccaatct cacgcaggga ttctgccacc tgtgcgacga tcttgcgagc ttcgacgaga
     9301 ctgtcttcgg cttccttttg tttgccgagc ttcgattgaa catctgcaag tccggctaac
     9361 gaagaccaaa gctgaaggtt ggcatcaatt tcctttgcca gcgaacaggc ttcggttaag
     9421 atttgaagcg cttcgtcgag tctgttcaat ccaagtaggg cgagtccctt caatcgcaaa
     9481 acatcgggaa catccactcg agtcaacgat ttcacctcag ccattaattc atcagcaagt
     9541 ttcaacacct cggcatattt gccttttgca tatgcaaact caaggtttgc aagacagaca
     9601 aaaatggtat agcgcgcata tggaatggtc gtaggctgta gcggcttatc accaatcgtt
     9661 tcaccagcta attcaagatc gcccttcatg atgtacatgc gtgcccggct tgccaaaggg
     9721 aatgatctcc atgcgggctg tctggcatct gcaatctgca agggctgaat ggtaagagat
     9781 aaacctttct cgaatgcacc acaataggca taggcccagc ccaactctga acgcagactg
     9841 atgctactgg caatcagtcc tgcttgatcg gcgagttgaa cactgcgttc ggcaagctga
     9901 atgccgcgac ccaattcgcc attctcaaaa tacacgaacg acatcaacat catgttatac
     9961 gcctgccccc aaagattatt gatttcttcg ctgatcttca gagccttttc acagcactcg
    10021 atgcctaatt caaattctcc accattgaaa tatgcctctg cctccgaacc ccaactatct
    10081 gccaacatga cttggtgatc gagcgatatc caaagttcac gcgcctgacg aacaaaagta
    10141 tgtgcctttt caaactcgcc tcggcaggta tgcagacggc agaggtcatt caatacaaaa
    10201 gcaagatatt cggtgttgcc agatttgcgt gccaaatcga gcgcggcttc accgtacggc
    10261 agggcttgat tgagccgctt ggaaaacaaa tacgtcagca tcaaattcca gttgaggcgc
    10321 gcctgtgcag aaatatcgcc aagcgcttgt gaaacttcaa gcgctcgaat taaagtcttc
    10381 tcactcagtt caggattatg cacttctgta aacgtggaat acaccgtggc ttttgccatg
    10441 agtgcggaca attccatgcc gcgttcatgg cgctcaattg caagagaaag catctcatca
    10501 tgatttgcca aagcctgctg atactttccg cttaattcca aaaccgtacc aagctgagta
    10561 tagagatgag caatctgctt caaatcagca gttgtccgtt ttgccacttc gagagcacgt
    10621 cgaaagtgcg ttgcggcttc ggcattggca tacagccgcg cagatttctc gccagatatc
    10681 agatcatatt tcaaagaacg atcatctccc gcgcggtaaa aatgatgagc gattagcggc
    10741 gcgaattctt cgatgcgatt cgcatgaatc tcttccaacg cttcgccaat ttgacgatga
    10801 agttcagcgc gtttcttgag caggatcgat tcgtaggtcg cttcctgaat caacacgtgt
    10861 cggaaagcgt attcgcccaa caacgcttca atgagactca catcctgcaa atatcggatc
    10921 tgtggatcaa gtccaccgct taaatccgtg agacgtcgga gaactcgcag gtcgaacaaa
    10981 cgcccaatca cggcggcatt ttgtaaaaca tgcttaggag tttcagccag gcgatcaacg
    11041 cgcgcgctca acacaccgcg caacgtattt ggcaatgaga tcttcgcttc atcaaccatc
    11101 gctttccaat ggttttcctc gcggatgatc tgttttgttt cgatcagcga gcgaatgatc
    11161 tcttcaataa aaaacggatt accctcacct ttgcttatga ttaaatttct aagggaaatc
    11221 ggcaactcat tagcatccag caggttgttc agcaatgtct cagtctgctc cgcctgcaag
    11281 ggcgcgagat caatcgagtg aaaagcatcc cccaatttct cacgcatctt ttggatcgag
    11341 tcccaactcg ccgcagtttt atcaggtcga gacatgcaaa taaaaaggat tggttgttct
    11401 gcgttcaaat ctgcaacatt gagcagcagg ttcaacgaag cctcatccgt ccaatgcaaa
    11461 tcatcgaaca cgatcaccaa gggactctcc accgataagc cgcacataaa tccacgtgta
    11521 gcatcgatca tgcgttgttt gagcgcatcg ccttgaaaac ccatcacaac ctttgcgctc
    11581 tcttcacttt caaccgcgag catcgcttct aaaaacggga tgtcaccgcc gggcaatgtg
    11641 cagcactcac acacatatcc caacttgtgg cgcacttccg caggcgaatc attttcatgc
    11701 gcttcaaccg attgccggat aatttgccgc catggataat aactgatcga actcgtatac
    11761 gataacgaat ctccccgcag ccagataaaa cgcgctcctt catcggattt tttcaactcc
    11821 gcgatcaatg tggactttcc aagccctgcc tcccctacta cggcgacgac tgatcctctt
    11881 ccccgatcca aatccttcaa ccgatcttta agagccgtca attgcgcgtc acgccctacc
    11941 aagggcgagg acaaaccttc aagtccgcgc aggtttccgg gcgttgattt tgctcccaac
    12001 acacgatacg ttttaatcgg tgccgatttc cctttgactt cgatctcacc cagggcttcc
    12061 acatcaaaga atggggcgac taatttatat gtttcatcgg aaatttgaat cgtgccgacc
    12121 tgcgcggttt gctccatccg cgccgcgaga ttgatcgcat caccgagcgc agtgtattcc
    12181 atccgcaaat ccgagccgat ttccccgaca acgaccaggc cagtattaat tcccacacgc
    12241 aggccaaagg caagcccata cttaaggcgg atttcctcgg cataggcttt gatcccctcc
    12301 tgaatttcca accccgccaa cactgcgcgc tgcggatcat cctcatgcgt gatcggcgca
    12361 ccgaagaagg caagaatcgc atcacccatc aggcgcggca cgagcccttc atatttgtag
    12421 atcggtcgga tcataaactc aaacacaccg ttcatgatct ccgaccagat ttcagggtcg
    12481 actttttcgg cggcggcagt cgagcccttc acatcgcaga acaacatcgt gacgacgcga
    12541 cgctcaccaa ccatttcatt ccgcgagcgt gccgattcaa gttttgttac caattccttt
    12601 gggagatacc gatccaattg aaaatttgtg ctcttcgctt cccccgttgc aactggctcc
    12661 ttcggaatca aacggcttcc atcccttgca ccagcaccct gcgcaagaga cgttccgcaa
    12721 ttcatgcaga atcttgcagc gtcaggattc gtcgtctgac aatttgggca tttcatattt
    12781 taccaattac ttaatcgatt ctagcacaat gcaacattct caccattgac cagctgcacg
    12841 tcacccggac gatccccttc gaaaattata taaaatttga caaggactat cccatggcaa
    12901 aatatcgcat catgtatggg aagcatatcc cgcaatcatg tactgtggaa ggtaatgagc
    12961 gcaccattaa gaaacagctc tcacaaaaga ttcaaggcaa aatctatgca tactcaatgg
    13021 cggagggctt aacgtccaca tcggattatg cagcgcaata caaacgaggg gctgtgagat
    13081 tcaacaatga aaaaacacaa ctaacatata atgacaaaat gaagaaatcg gctttttact
    13141 tctgtatcgt ttggttagaa attttgactg cctgtgcgcc agcaacggtt ccagctgtag
    13201 agttgcaagt gcccgcggta acggctctta atggaaatcc ctcaccggat tttgctgccg
    13261 cggatggctc tcgccgactt gcctttccta aagacttcgg tccccatgaa gaattccgta
    13321 ccgagtggtg gtattacaca gggaatttgc aaacgtctga aggtcgacat tttgggtttg
    13381 aattgacgat ctttcgcgtc agtttgctgc cgccaacgat tgaacttcct tctgagtctc
    13441 agtggtatga ccacagcctg tactttgccc attttgccgt cagcgatatt gcgaacgaaa
    13501 aattctatgc ttttgaacga tattctcgtc cgggaccggg attggcaggc gcgcaggttg
    13561 aaccctatcg agtttggatc gaggattgga acatcattga acaggtgccg ggcatatatc
    13621 acttgcaagc ggctcaaacg gatattgcca tcgacctaac cctgaccgac gaaatgggag
    13681 tcgtgttaca cggagagagt ggttatagtc gcaaagggga ggatgtcacg agcgcttcgt
    13741 attactacag ccagccgcgt ctgcgtgctg aggggttttt agaagttgat gaagttcgat
    13801 attcggtcac aggattggta tggaaggatc acgagtttag taccagtgta ttggatgaaa
    13861 atcaaatcgg ctgggactgg ttctccctgc aattcgaaga tggcaaagca ctgatgcttt
    13921 ttcaattgcg cgagcgtgat ggaggaacat ccgcttcctc cagtgggacg ttgatcgaca
    13981 tgaatggaac ccctcaacat ttgcagaaga ccaactttga gattatcgtg cgcgaccagt
    14041 ggcagagtcc acatacgcag ggagtatatc cctctgcctg ggaggttaat ttaaaggatc
    14101 tgggttgccg attggaaatt cacccctgga tggcagatca ggaattgcac ttcccaacag
    14161 taacctattg ggaaggggca gtacgttttg aaggaacctg tgaaaatgga gagtccgtgc
    14221 gcggaaacgg gtatgtggaa ctgacgggct attctggtaa tgtaccgctg ccttgaagta
    14281 attgattaaa agaagaccct actttgaaaa gctgtgaaga acattttatt tcttcacagc
    14341 ttttcaagta taccgactat tatattaagg tgttggtgtg cttgtcggcg taaaagtcga
    14401 tgtggcggta gaggttgggg aagcaaccgg cgtgttcgtt aaactactgg tgggcgttgc
    14461 cgacccggtt ggagggacga taagcgtaga tgtggcagta gaagtcagag aagttgccgt
    14521 tgccgtgatg acgatcggcg taagcgttgt agtggaggcc gttgccgtcg ttaccagggt
    14581 cgatgtaact gtaggtgttc cggtcactag agcgcaggct tgcaaagtaa gctgcacttc
    14641 tgtgcaatta ttctcatcca tacaaacatt catggtgatg gttgtgttct cttctgcacg
    14701 gcaaagcacc agcttcttgt ctctaaatct gtcgattacg cggcagccca gacgatcctc
    14761 gttatcatca tcgtcttcat cgttatctac acgagccagt tgtcctcgta ttgcaccatc
    14821 tggaaattct tcgttgtgga cgttgacgta gtattgctca gggttttcta tgatattttg
    14881 gagtaaagca ttatctacat cgctaaggca tccactcgat gagccttctg ctggtgcggg
    14941 atcaagcggc accacgacct cccctgcgac gccttcggca ccttcatgaa tatgcgcggc
    15001 cgtggccggc ccaatattgg acgcggtgag atcaaagcac atttcatctg cctccaggct
    15061 taatctgatc atggcagttc cgctaccatc cggatccccc gggcccggta cttctgccgc
    15121 gccactcaac tgtgcggtgc gccgatggcc accatcaccg tcaccatcgt catcatcatc
    15181 gtttgccaac tctggcggtg aagtgactct aaagctggct tctcttggga ttaccagttg
    15241 ggcatgggcg atcgtatcca cacagtattg gcaatccaat tgaattagct caagtccggt
    15301 cactgggatg gacggggtca gagtcgtatc tgttgcggtg gccagtggtg tagcacttgt
    15361 atcggtcacc acagcgatgg gggaactggt ctccgcttgc gcagccggag ttaatgtctg
    15421 ttcctccact ggagcacagg cggcggccaa tagaacgacc gccactactc caggtaggaa
    15481 taagctcgaa atccttttca tgtattgttt tctcctttcc cgtgcagagg ctgctgttcg
    15541 tattgggcgt gctgatatct ttacagatgg gtaaaaggtg aacgaccccc tcaggtgtgt
    15601 cgtgcaaaac ccaatttcaa ctttgggttt tatagatttt gcttagatat cgaaggtcag
    15661 tgtgtgcaac cggcagtaga tccctgccag ggatgtattt gtttcaggga tactatcgga
    15721 aaccgaaccc gccgacaatt aggggtttcc ctgtataggg tagggatgtc ctgccctctt
    15781 ccttccattt ctcacaaact gtgggagaaa gttcttagaa gtctttaagg tcagcctgat
    15841 ttctaatgta agttctaatt cttagatagt atgacttcga gaaagcaccg tatgtacttt
    15901 ggaaggcaac ggatatttcc cctgcggatt attccagaga acgactggtt tttcggattc
    15961 caacggtatc tctctaatga gaattgcact cagccgctgg taatgattga agaaatcagc
    16021 acggtcgcga aagccgtgcc tttgcaaaac gtttgggtca ttggtttccg ctcgtttttg
    16081 gatgctgagc ataatggcat gcagatcact ttgagtgaga tggtaagcct caagaccgac
    16141 cagcagaaga ttgctgcgct tcgagagcaa ttgtcccaat ttgctgcata gcatatcagt
    16201 gaaacggtcc atccaaggta acccatccgc ctgtttctcc gctccgggag atgttcttgt
    16261 tcctattgaa cgcagccggg tgacctccac cataaaagtg aggctggtgg tgaatgctac
    16321 tgcaaagtca gggctgcgcc ccagatccgg atgctggggt tcataggcca cgcccagagt
    16381 cggctcccgc acgagcaaat aagctgtttc caattccaac tggagatcac gcaggttttc
    16441 agatccctgg accgtgcgaa ttttcttccg aatttttgta tggtgggcgt taacaaaagt
    16501 cgtgaatcga cgcgaggaac ttaaccatga ggtcatgggt ccagtgaggg gattgggttt
    16561 cccatcgaag aggtaactaa gcaattcatt gaccaacatc gccgcgttcg ctcaaaggag
    16621 gattatcggt taaccataga tggattgatc caaaattctt cacgttctca caatttggat
    16681 ttacaatggg gcttcactcg cggacagtgg acaattggga tgcttgaatg aacttgagcg
    16741 gttggagtag ccatgagtga tgatggtacg ccagtaatca tatccaaaaa atcttcttga
    16801 atagcttcat gatacctgtt ttccgagtcg cttcatcaat gcgactcaga aattgccgta
    16861 cctgattgga attgtcaggg ttaagcatct tgcaaaagga gcggggtaaa tcccgccata
    16921 atttaatggc cccgggacct tcatgactca cgaaatgaat ctacaagcta tgccgttgga
    16981 tgatcttgcg caacgatgcg cgcaggagac aatcctttac tttaaatctc gcatccacga
    17041 taccagctat tgttttgaac tgtttcgccg ggcggtcact gaacgcagtg aactggcatg
    17101 gagcgcaata ctcgcccaat ataaaccgtt ggtggcacgc tgggtgaatc gacaggcaga
    17161 taaacatccg gattttccgc ttgccaggga ggaggagcag gatttcgttg atgaggcgtt
    17221 tatccgattt tggcattatt tcacttctga aaagttccat aattccaaag gactagacgg
    17281 tattctcaag tatctgaaaa tgtgtgtaaa tagcgcagtt ttggatatcc tgcggaaagc
    17341 gcagcatgtg cagttcgagc agaaaccgga aggtgaagag gaggacgatg aaaccaatca
    17401 gggtgaaagc ggacctactc ccgaggaatc gcttcaaagc gatgaggtat ggaaagcgat
    17461 caaggcgcgg ctaaaagatg agaaagagca cgtagtcatc tatgcctcat tttttcttgg
    17521 actatcgcca cgtgagatct ttatggagta tcctgatgta ttccgtagca tagacgagat
    17581 ttaccagtgt aaggcgaacg tgctggcgcg gcttcagcgt gattcggaac ttcgagattt
    17641 gttcaaataa gatgccagaa aaaatcaaaa agcggcgttt ataagatcag aagtgagaaa
    17701 aagtttctta cttgaacggg tctaatttgc cataaccgta tgtcggccac ctgccatcca
    17761 tatcaaaggc agccagggcc aacccgataa gaggtagctt atgaaatgta tcacttcgcc
    17821 cgagttggat gataccaaaa tagtcagtta cgtagagggc gaagctgatg cag
//
LOCUS       DASTJP010000422         9771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_779516, whole genome shotgun sequence.
ACCESSION   DASTJP010000422 DASTJP010000000
VERSION     DASTJP010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779516"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFQ23_12205"
     CDS             <1..272
                     /locus_tag="VFQ23_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907405.1"
                     /translation="NQRFFDNFLFANIGKVLQKGKPHLQRIGLSNPGEQIMSSVLEDG
                     HNIALNVILLPAIRDVTPSIVVKPNYGWAQHGHADSSRLYKLRQI"
     gene            complement(325..909)
                     /locus_tag="VFQ23_12210"
     CDS             complement(325..909)
                     /locus_tag="VFQ23_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9907406.1"
                     /translation="MPFKFDPKKHHRRSIRLKGYDYSQPGAYYVTIVTWHREFLFGEV
                     VNGEMRLSKFGLVAKQQLEKLSKRFPNIEFGAFVIMPNHIHVIIFITDGSRGTAGRFT
                     VKITRTLRRAPTPERYQRPVKGSIPTIIRSYKSAVAYRINLMRRTEGVPVWQRNYYEH
                     IIRNEQDLQNKTDYIEANPMLWDEDDENPINVLP"
     gene            930..2072
                     /locus_tag="VFQ23_12215"
     CDS             930..2072
                     /locus_tag="VFQ23_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9907407.1"
                     /translation="MTTPLNIVQTQIPTGMIDLGLGNPPLSLLPLDMIRLAAQEQLSK
                     NDTSFLQYGTEQGDGYFRTALGNFLSKGYGFPVEPERLFITTGISNGLDLLCSLFTKA
                     GDTIFVEEPSYFLALKIFADHQLQVVSVETDDDGIILEALKEKLAQHKPRLLYTIPTY
                     QNPSGHTLTQERRARIVELSQQFDFLVLADEVYQLLSYEGNPPKSFGVFAEAENVIAL
                     GSFSKILAPGLRMGWIQAHPNRISRMSGSGLLDSGGGLNPFTSALLRGVIESGNLEKN
                     IDNLIDVYRSRIQIMDEALREHLPEAKYALPQGGYFFWLRMPEDVDGTELRKKARAFN
                     VDLRQGALFSSRNALKNYIRLCFVFHEVDEIKEGIRRIKECLKSSD"
     gene            complement(2082..4412)
                     /locus_tag="VFQ23_12220"
     CDS             complement(2082..4412)
                     /locus_tag="VFQ23_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9907408.1"
                     /translation="MTQMENPVPTSEPKNRSTGAVVLFLLLVVPMPLCLLIYHFTLWT
                     TEQSAIASGSLASLAWAGLLGLAAQAVVLSGAIAALWYFTKDDRFKPVYSGWLAASLI
                     AFPALLLRLLGPNNDQPGSLLQIFICVIASLIFARVRNARIDLRGNNKSFAFFLAALG
                     VAPFAMDGALGSPTDALLNLLAGLSFGWLAALLMEATTENKFLDAFGMGVVLSLLGSA
                     IGYDGAQLILLAILPSFAFAASALMPSRVAGMILTGLLAAAGLIFFDPTELTIVLGDI
                     AGIALKAVSFAVGIGLVVGLIALVIRYITDANQGSNITRILGAVSAVAAWVVLVILFF
                     ATGNRGFYGDRLFVILNDQADLSEISQIQDIDERRTAVYQTLTAHANETQAELRNTLN
                     SVGVTYTPYYLVNAIEVRGGTLVRLFLSTRPEVDRVISSPRLRPASQGESPITNISDF
                     TPTGVQWNVSMIGADKVWEEFGARGEGIVVGQSDSGADLNHPALNETYRGNNEGDDYN
                     WLDPWNGQPSPYDVGGHGTHTLGTVLGQNGIGIAPDATWFACANLVRNLANPALYLDC
                     MQFMLAPYPQDSDPFVDGDPTRAADVLNNSWGCPELEGCDPNALLAGANNLRAAGIFV
                     VVSTGNDGPNCSTVDAPLSLYDSVFSVGAMDQLGNVADFSSRGPVTADGSGRIKPDII
                     APGVDVLSALPGGSYGSNSGTSMAGPHVAGAVALLWSADPTLIGDIDRTEQLLIDSAN
                     PYTGNRNGCFEGTTPNAAFGYGVLDVYEAVKMALGR"
     gene            complement(4464..5114)
                     /locus_tag="VFQ23_12225"
     CDS             complement(4464..5114)
                     /locus_tag="VFQ23_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907409.1"
                     /translation="MLKPVRWNTFLRDFIVIQIGFAIFGLSIALMIRSHLGTTPWAVL
                     EVALSQLTGIRPGRMSILVGAVVLIGALALREKIGWGTISNILFIGLWEDMFLSYIPP
                     VQDNLILQIAMLLGAILMMGFSTAIYIGVNAGAGPRDSLMLAFHRTTGISIRLGRAII
                     EVIVVAIGWLMGGPLGFGTVVFALTIGPAVQWGFKVFRVRPHKPEEIIEPAEAAAD"
     gene            5327..5782
                     /locus_tag="VFQ23_12230"
     CDS             5327..5782
                     /locus_tag="VFQ23_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907410.1"
                     /translation="MPQIIEVQREQFTISTDPARLDTDVIVDFLARAYWAKGRPRERT
                     ERALTNSLVFGLYEGKKQIGLARVISDYAVFAYLCDVFIHEDYRAYGLGKWLMETVHA
                     YPDLQGLRRWTLATRDAHGLYRQFGWDALANPESWMEILRPFPGEGQDS"
     gene            5779..6996
                     /locus_tag="VFQ23_12235"
     CDS             5779..6996
                     /locus_tag="VFQ23_12235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907411.1"
                     /translation="MNVILKPGREKSLLRRHPWIFSGAIHAADEHIPSGSTVDLLSST
                     KQFLAHASYSPNSQIRARVWTFDDEKVDKGFFQKRIRAAIAVRSPLLLGGGLGVRSDS
                     YRLIYAESDGIPGLIVDRYGDVLVLQSLTAGSEFWKETFADILIEETGLTTIYERSDA
                     DVRELEGLGPKVGLLRGHLRSLRPGSGQAFMFPITEHNLKFNVNLQSGHKTGFYLDQK
                     MNRLRVRELAKDKDVLDCFCYTGGFTVNALAGGAKSVLSIDSSEDALALARENIVLND
                     LPSKKATFLDGDVFQLLRKFRDENRSFDMIILDPPKFAPTAAQAERAARGYKDINLYA
                     FKLLRPGGLLITFSCSGGIDAGLFQKIVAGAALDAGVDAQILEHVSQNADHPISLNFP
                     EGAYLKGLVCLRK"
     gene            7137..8315
                     /locus_tag="VFQ23_12240"
     CDS             7137..8315
                     /locus_tag="VFQ23_12240"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9907412.1"
                     /translation="MKHYKYLIIGGGLAGDGATRGIRELDTDGSIGLISAEPDPPYMR
                     PSLSKGLWKGRPVEKVWRKTEERAELHLNRKAAQLDPTKKHVRDDGGDEYTYDKLLIA
                     TGGSPRHLPFGNGNIIYYRNFQDYKHLRNLTESGNHFVVIGGSFIGSEIAAALTMVGK
                     KVTMIFPGDSISDKIFPIGLSLFLNDYYRQKGVEVLANESAASVQKDGNRLMVRTGSG
                     RAIEADGVVAGVGIQPNLDLAKEVGLKIDDGIIVNEYLETSIPDIYAAGDAANFFYSA
                     LEKRTRVEHEDNAVAMGKLAGRNMAGATDPYTHTPMFYSDLFELGYEAVGEMSSKMKT
                     IEDWQEEPFKKGVVYYMDDDRVRGVLLWNVWKKVEDARALIKEEGPFKEDELKGRITG
                     "
     gene            complement(8351..9160)
                     /locus_tag="VFQ23_12245"
     CDS             complement(8351..9160)
                     /locus_tag="VFQ23_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907413.1"
                     /translation="MKLYKFWTVEKQKVLIHGEEQIITCYGGSNVSEEEARSRAKDKA
                     EKIRRKIAGERHVFDRYEAEIREEILQMVDDHSAITRNRYGAQVLNTENLMILDIDKP
                     KPAAGGLGGLFKKKDTRSPKEQIFEMVRNLATSAKYKGYGFRTYETFQGARVIVLGGS
                     FDPRSSPSKDMMDEFNCDPLYTTLCNKQGCFRARLTPKPYRMKMKPYKVNFPRESTDP
                     EFQRWLSDYEGESRNYSVCKFIEQVGASHYIDKVVQIHDEITGVGYPQRLA"
     gene            complement(9194..>9771)
                     /locus_tag="VFQ23_12250"
     CDS             complement(9194..>9771)
                     /locus_tag="VFQ23_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907414.1"
                     /translation="MPRPAKENEIRARFYALFEQINQQLFEMATEHPGALAPETTLIV
                     CQLREFESNKYLYWSSFGDSYLLLLRDNKLIQLNTLNAYWLGMLSRMSETSLTRQLTL
                     KGLFGESTYIGVADGLETGIEKLQSGDMIILCTDGLIGSDQGIPEGVEMAFTAILTGL
                     ERPENKVKNLLQSALDRFEIDNISCILLQID"
BASE COUNT         2554 a         2485 c         2380 g         2352 t
ORIGIN      
        1 tcaatcaacg ctttttcgac aatttccttt ttgcaaacat cggcaaagtc ctgcaaaaag
       61 gaaaacccca tcttcagaga atagggctca gtaatccggg cgaacaaata atgtcctcgg
      121 ttctcgaaga tgggcataac attgctctca atgtcattct actaccagct atacgagacg
      181 taacaccgtc cattgtcgtt aaacccaact atggatgggc acaacacggg catgcagata
      241 gctcacgctt gtacaaactt cgacaaatct gagatgatgt tatgtggcaa ggtgaaatga
      301 cgaataaacc tcggtgggtc gcccttatgg taagacattt atcggattct catcatcctc
      361 atcccataac atggggtttg cttctatgta atcggttttg ttttgcaaat cttgttcatt
      421 gcgaatgatg tgctcgtaat aattcctttg ccaaacagga acaccttctg ttctacgcat
      481 caaattgatt cgataggcaa ccgcagattt atatgaacgg ataattgttg gaatagaacc
      541 ctttacaggt ctttgatatc tttccggcgt aggggcacgg cggagagttc gagtgatttt
      601 gacggtgaac cgtcccgccg tgcccctact accgtccgta atgaaaataa tcacatgaat
      661 atgatttggc ataataacaa atgcaccaaa ttcgatattt ggaaatcgtt ttgacaattt
      721 ttccaattgt tgctttgcta ccaagccgaa cttgctcaac ctcatttccc cattcaccac
      781 ctcgccaaag agaaattcgc gatgccacgt aacaatcgtc acataatacg cacctggttg
      841 ggaataatca tatcccttca accttatgga gcgcctgtga tgcttcttcg gatcgaactt
      901 aaagggcata atgcgattat actttcgcca tgaccacacc ccttaacatc gtgcaaacgc
      961 aaatcccgac gggcatgatc gatcttggcc tggggaaccc accgctctcg ttgcttccac
     1021 tggatatgat acgccttgcc gcgcaggaac agttatcgaa gaacgacacc tcctttctgc
     1081 aatatggaac tgaacaaggc gacggatatt ttcgaaccgc tctcggaaat ttcctgagca
     1141 agggatacgg ttttcctgtc gagcccgaga gactattcat taccacaggc atatcgaacg
     1201 ggttggacct gctgtgcagt cttttcacga aagcaggcga tacgatcttt gtagaggagc
     1261 catcctattt cctcgccctg aaaatctttg cggaccatca gcttcaggta gtttccgttg
     1321 agacagacga tgatggaata atccttgaag cgctcaaaga aaaactggcg caacacaaac
     1381 cgaggctttt atatacgatt cccacctatc agaatccaag cgggcatacg ctcacacagg
     1441 aacgccgcgc acggatcgtg gaattgagtc agcaattcga ctttttggtt ctggcagatg
     1501 aggtctacca gcttctaagc tatgagggga atccgccaaa atcgtttgga gtctttgccg
     1561 aggcagaaaa tgtgatcgcg ctgggatcgt tttcgaagat ccttgcgccg ggtctacgga
     1621 tgggctggat ccaggctcat ccgaaccgaa tcagccgtat gtcaggcagc ggattgctcg
     1681 atagcggcgg agggttgaat ccgttcactt ccgcactttt gcgcggggtg atcgaatcgg
     1741 gcaacctgga aaagaacatt gacaatctga tcgatgttta tcgctcacgc attcagatca
     1801 tggatgaggc cctgcgcgag catcttcctg aggcaaaata tgctcttcca cagggcggat
     1861 atttcttctg gcttcgtatg cctgaagatg tagatggaac ggaattgaga aagaaagcgc
     1921 gagccttcaa tgttgacctt cgacagggcg cgctgttttc cagccgtaat gcgttaaaga
     1981 attacattcg tttatgcttc gtatttcacg aggttgatga aatcaaagaa ggcattcggc
     2041 ggataaaaga atgcttgaaa agcagcgact aagtacgtcg cttatctacc cagcgccatc
     2101 ttcaccgcct cataaacatc caaaactcca taaccaaacg cggcgttggg ggtcgttcct
     2161 tcaaaacaac catttcgatt gccggtatac ggatttgcac tatcaataag aagttgttcc
     2221 gtacgatcga tatccccgat caacgttggg tcggcagacc acagcaaggc caccgcgcct
     2281 gctacgtgcg gtcccgccat ggatgtgccg ctattcgagc cgtacgatcc accgggcaac
     2341 gcggaaagaa cgtccactcc gggagcgatg atatccggct tgattcgacc cgatccatcc
     2401 gcggtgacag gtccacggct gctgaaatca gctacatttc ccaactgatc cattgctccc
     2461 actgagaaaa cagaatcata caaagataac ggcgcatcaa ccgtgctgca attgggtcca
     2521 tcattccctg tcgaaaccac gacaaaaatg ccagcggcgc gcaggttgtt cgctccagca
     2581 agaagcgcgt tcgggtcaca gccctcgagc tcagggcagc cccatgaatt gttcaagacg
     2641 tccgcggcgc gggtggggtc accatccacg aacgggtcgc tgtcctgcgg ataaggtgcg
     2701 agcatgaact gcatgcaatc cagatacaag gcgggattgg caagattacg aactaaattt
     2761 gcacacgcaa accaggtggc atctggagca atgccgatcc cattctgacc caggacagtg
     2821 cccagggtat gtgtgccgtg cccgccaaca tcgtagggtg agggctgccc attccacggg
     2881 tcgagccagt tgtaatcatc gccctcatta ttaccgcgat acgtttcatt caaagcggga
     2941 tgattcagat cagcgccact atccgactgc cccaccacga ttccttcgcc gcgtgcccca
     3001 aattcctccc agactttgtc agcaccgatc atggacacat tccactgaac gccagttgga
     3061 gtaaagtcac ttatgttagt gatgggactc tcaccctgtg aagccgggcg caggcgcggg
     3121 cttgaaatga cgcggtccac ttcaggacgc gtcgaaagga aaagacggac gagagttccg
     3181 ccgcgcactt ctattgcatt gacaaggtaa tagggagtat atgtcacgcc tacgctgttt
     3241 aacgtatttc gtagttcagc ctgtgtttca ttggcatgcg ccgtgagcgt ctgatagacc
     3301 gcagtccgtc gttcatcaat atcctgaatc tgacttatct cggaaagatc cgcctgatcg
     3361 ttcaaaataa caaacaaacg gtcaccataa aaaccgcggt tgcccgttgc aaagaaaagg
     3421 atgacgagaa ccacccatgc ggctactgcg cttaccgcgc caagtattcg tgtgatattg
     3481 gaaccctgat tggcatctgt aatgtaccga atgaccaaag cgatcaggcc aactacgagc
     3541 ccgatcccaa cagcaaacga gacggctttc aacgcgatcc ccgcgatatc gcccagcaca
     3601 atcgtgagtt ccgtggggtc aaagaaaatc aatcccgccg cggctaatag acctgtcaga
     3661 atcatcccgg cgactctgga tggcatcagt gcagacgctg caaaggcaaa tgagggaaga
     3721 atagcaagca gtatcaattg cgcgccgtca tatccgatgg cggaccccag cagcgacagc
     3781 accacaccca ttccaaatgc atcgaggaat ttattttcag tggtggcttc catcaacaac
     3841 gcggcaagcc atccgaaaga caagccagcg agaagattga gaagtgcatc ggtcggcgaa
     3901 cccagcgcgc catccatagc gaatggagcc acgccaagtg ccgcaaggaa aaatgcaaac
     3961 gacttgttat ttcctctcag atctattctt gcattacgga ctcttgcaaa gatcaaagac
     4021 gcgatcacac atatgaaaat ttggagaagg gagccaggtt gatcattgtt aggtccgaga
     4081 agccgcaaca acaaagcagg gaatgcgatg agcgacgcgg caagccaacc ggagtaaacc
     4141 ggtttaaagc ggtcatcttt tgtgaaatac cataacgcgg cgatcgctcc gcttaacacg
     4201 accgcttgag cagcaagacc tagtagtccc gcccacgcca ggctggccaa actgccagat
     4261 gcaatagcag attgctccgt ggtccaaagc gtaaaatgat aaataagcaa acacaatggc
     4321 atgggaacta ccagaagcaa aaacagaacc actgcaccgg tggaacgatt ttttggttca
     4381 gatgtgggaa ccgggttttc catttgggtc ataaagtctc caggattccc tgattgtaca
     4441 acaacgggac cagcgccgaa tggctaatca gccgcagcct ctgccggctc aatgatttct
     4501 tcgggcttat gtggccgtac ccggaatact ttgaatcccc actgcaccgc gggaccaatc
     4561 gtcaaggcaa agacaacggt tccaaatccc aatggtccac ccatgagcca tccgatggca
     4621 acaacaatga cttcgataat ggcgcgtccc aggcgaatgc tgattcccgt cgtgcgatgg
     4681 aacgccagca tcaggctatc ccgcgggccc gcaccggcat tcacgccgat ataaatggca
     4741 gtggaaaacc ccatcatcaa gattgcgccg agtaacattg caatttgcag aatcaagttg
     4801 tcttgaacag gcgggatata agataaaaac atatcttccc atagtccaat gaagaggata
     4861 ttcgaaattg ttccccaacc gatcttctcg cgcaaagcga gcgcgccaat caacacaaca
     4921 gcgccgacga gtatactcat ccggcccggg cggattcccg tcagttgaga taacgccact
     4981 tcgagcaccg cccagggagt cgtccccagg tgcgaacgga tcatcaaggc aatcgaaagc
     5041 ccaaagattg caaaaccgat ttgtatcacg ataaagtcac gtagaaaagt attccaacga
     5101 acaggtttca gcattcagtc tccttatatc gcgttgagca gagctaaagg cgctgacgaa
     5161 acacaagttg cctacatact cgcgcttcga ctgcatgcct cgacttcacc tgcactcccc
     5221 ggcaccgacc aaccggggcg gtgtgcacac aggtgcaagt gtcactcggc acttcgctca
     5281 gcgtgaaata taactttgac gataacacgt atgatagact tacgccatgc cccaaatcat
     5341 cgaagttcag cgcgagcaat tcacaattag tacagatccc gcgcggcttg acacggatgt
     5401 gattgtcgat tttctggcgc gtgcctattg ggcaaagggc cgtccgcggg aacgcacgga
     5461 acgcgcacta accaactcgc ttgtatttgg gctctacgag ggaaaaaagc agatcggtct
     5521 tgcacgcgtc atcagcgatt atgcagtttt cgcctatctt tgcgatgtgt tcattcatga
     5581 agattatcga gcgtacggac ttggcaaatg gttaatggaa acggtccacg catacccgga
     5641 tcttcaaggt ttgcgccgct ggactctcgc aacacgcgac gcgcacggac tatatcgcca
     5701 attcggttgg gatgcgctgg caaaccccga aagctggatg gaaatcctgc gaccctttcc
     5761 cggtgaaggg caggattcat gaacgtgatc cttaagccgg gacgtgaaaa gtccctgctt
     5821 cgccgccacc cctggatttt ttcgggcgcg attcacgctg ctgacgagca tattccatcc
     5881 ggctcgaccg ttgacctgct ctcctctact aaacaattcc tcgctcacgc ctcctattca
     5941 ccgaattccc aaatccgggc gagagtatgg acgtttgatg atgaaaaagt tgataaggga
     6001 ttctttcaga agaggatacg cgctgcaatc gcagttcgtt cccctctcct tttaggaggg
     6061 gggttagggg tgaggtcaga ttcctatcgt ttgatttatg ctgaatcaga tggaatcccc
     6121 gggttgatcg ttgatcgcta tggcgatgta ttggttctac aatcccttac ggcgggcagt
     6181 gaattctgga aggaaacctt tgcggatatt cttatcgaag aaactgggct cacaaccatc
     6241 tacgaacgct cggatgccga tgtgcgcgaa ctcgaagggc ttggaccaaa ggttggttta
     6301 ctacgagggc atcttcgctc ccttcgaccg ggctcagggc aagcttttat gtttccgatt
     6361 accgaacaca atttgaaatt taatgtgaat ttacaaagcg gacacaaaac cggcttttat
     6421 ctcgatcaga aaatgaaccg tctgcgtgta cgcgaactcg caaaagacaa ggatgtgctg
     6481 gattgttttt gctacactgg cgggttcacc gtcaacgcgc tggctggcgg ggcaaagtcg
     6541 gtactttcaa tcgattcctc tgaagatgca cttgcgctgg ccagggaaaa tattgtattg
     6601 aatgatttgc cttctaaaaa ggcgacattc cttgacggtg atgtgttcca gctcttgcga
     6661 aaatttcggg atgagaatcg ttccttcgat atgatcattc tcgacccacc caaattcgct
     6721 cccaccgccg cacaggcaga acgagccgcg cgtggctata aagatattaa tttatacgcc
     6781 ttcaaactac tgcgacccgg tggactattg atcactttct cttgttccgg cgggatcgat
     6841 gcgggattat ttcaaaaaat cgtggcggga gctgcgttgg atgcaggtgt tgacgcgcaa
     6901 atccttgagc atgtatcaca gaacgcagat catcctattt cgttaaactt cccagaaggg
     6961 gcctatctca aaggattggt ttgtttgagg aagtaactta tcattatgtc attgcgagga
     7021 gcgaacgaca gtaagcgacg aagcaatctc atgatttcat tcaagtatta tattgcaggt
     7081 gagttgaggt tgttcgggct tcgcgctcgc aatgacctct agaagaggat aaacccatga
     7141 aacattataa atatttgatt atcggtggtg ggctggcggg tgatggggca acccgcggga
     7201 tccgtgagtt ggataccgat ggttcgattg gcttgatcag cgcagaacca gaccctccct
     7261 atatgcggcc cagtctctcc aaaggtttgt ggaagggtcg ccctgttgaa aaagtctggc
     7321 gcaaaaccga agagcgcgct gaactccatt tgaatcgcaa ggccgctcaa ctcgacccaa
     7381 ccaagaagca cgtgcgtgac gatggcgggg atgaatatac ctatgataaa ttattgatcg
     7441 ccacaggcgg ctcacccaga catttgccat ttggaaacgg caatattatt tattatcgaa
     7501 attttcagga ttacaaacat ctgcgcaacc tgaccgagag tgggaatcat ttcgttgtga
     7561 tcggcgggag ctttatcggt tctgagattg ccgccgcgtt gaccatggtc ggcaaaaaag
     7621 tcacaatgat ttttccgggt gattccatca gtgacaagat cttcccaata ggtctctcgt
     7681 tgttcctgaa tgactactat cgtcaaaaag gtgtagaggt gctggccaat gagtctgcgg
     7741 ccagcgttca aaaagacggg aacaggctca tggtccggac cgggagcggg cgcgccatcg
     7801 aagcagatgg agtggtggcc ggcgtcggca tccagccaaa tttggatctg gcaaaggaag
     7861 ttggtctgaa aatcgacgat ggcatcatcg tgaacgaata cctggaaaca tccattccag
     7921 atatctacgc agctggtgac gcagccaact ttttttattc tgcgctggag aaacgtaccc
     7981 gcgtggagca tgaagataac gccgtggcaa tgggaaagct tgcgggccga aacatggcag
     8041 gcgcaactga cccatatacg cacactccaa tgttttattc agatctgttt gagcttggct
     8101 acgaggctgt cggcgagatg agcagcaaaa tgaaaacgat tgaagactgg caggaagagc
     8161 cgttcaagaa aggcgtcgtc tactatatgg atgacgatcg cgtgcgaggc gtgttgttgt
     8221 ggaacgtttg gaaaaaagtc gaggatgcgc gtgcactgat aaaagaggaa ggaccattta
     8281 aagaggatga gttgaagggg aggataactg gttaatggta gggacacagc gccgctgtgt
     8341 ccctactaaa ttacgccaac ctttggggat atccgactcc cgtgatctca tcgtgtatct
     8401 gtacgacctt atctatataa tgactcgcgc caacctgctc gatgaacttg caaacactgt
     8461 aattcctgct ttcgccttca tagtcagata gccagcgctg gaactcaggg tccgtactct
     8521 ctctggggaa attaacttta tacggtttca tcttcatacg gtagggtttg ggcgtcagcc
     8581 tggctcggaa acaaccctgt ttgttgcaca acgtcgtgta gagcgggtcg caattaaact
     8641 catccatcat atcttttgat gggctgcttc gcgggtcaaa acttcctccc aacacaatga
     8701 ctcttgcgcc ctgaaaagtt tcatatgtac gaaaaccata ccctttatat ttcgcgctcg
     8761 tggcaaggtt cctgaccatt tcaaagattt gctccttggg cgaccgcgtg tcctttttct
     8821 tgaacaatcc acctaatccg cctgcagccg gtttgggttt atcaatgtcc aggatcatca
     8881 gattttcagt gttcaaaacc tgggcgccat agcgatttcg agtgatggca gaatgatcat
     8941 cgaccatttg caggatctct tcccgaattt ccgcctcata tctatcaaat acatgccgct
     9001 ctccggcgat ctttcgcctg atcttttcgg ctttgtcctt cgctctggat cgggcttcct
     9061 cttcagaaac atttgaccca ccgtagcagg taattatctg ctcctcacca tggatgagaa
     9121 ccttttgctt ttcaaccgtc cagaatttat agagcttcat tgtttctctc ctgtaagata
     9181 ggcgtgatga gggctaatca atttgaagca gtatacagga tatgttgtct atttcgaacc
     9241 tatcgagagc agactgcaat agattcttta ctttattttc agggcgctcc aggccggtca
     9301 aaattgctgt aaaggccatt tctacgcctt cgggaatacc ttgatcagaa ccaattaatc
     9361 catcggtaca taatataatc atatcgccag actgcagctt ttctatcccg gtttcaagcc
     9421 catcggcaac accaatgtac gtggactcgc caaacagtcc tttcaaagtc agttgtctcg
     9481 tcaggctggt ttctgacatt ctggaaagca ttcccagcca ataggcatta agtgtgttaa
     9541 gttggattaa tttattatcg cgcagcaaca acaggtacga gtcgccaaaa cttgaccaat
     9601 agagatactt gttggactca aactccctta attgacaaac aataagcgtc gtctccggtg
     9661 cgagagcgcc aggatgttcc gtcgccattt caaataattg ctgattgatc tgttcaaaca
     9721 gtgcataaaa acgcgctcga atttcattct ctttggctgg gcgaggcatc a
//
LOCUS       DASTJP010000423         5748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_952536, whole genome shotgun sequence.
ACCESSION   DASTJP010000423 DASTJP010000000
VERSION     DASTJP010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952536"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFQ23_12255"
     CDS             <1..632
                     /locus_tag="VFQ23_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907415.1"
                     /translation="MADQFSLRVYSYLDRMQPQYAAFVGTITQGDLPTEGMASLYVEV
                     APGNEVFRLVDIAVKSTEAKPGAQLVEREFGMFEVHSRSQSEVLEAGRIVLDRLGLKP
                     EERIKPFIASVQIITNVDPYQAQLLNRFRRGSMLVPGETMLVLECAPAAYINFACNEA
                     EKGAAIKILHVSSVGRFGRMWLSGSEAEILTARNSAVKALEGLDGRPEK"
     gene            734..1072
                     /locus_tag="VFQ23_12260"
     CDS             734..1072
                     /locus_tag="VFQ23_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907416.1"
                     /translation="MPKIFYTERDIDDLKARGITSLDVTDNIVLTDLAVERAMKHEIK
                     VNRVEHSAPMASYSPSVNTYAAYPRETNNSVNAELKEKIKAAVLARLNGQVDAALLDA
                     VITKVLAGMK"
     gene            1247..2335
                     /locus_tag="VFQ23_12265"
     CDS             1247..2335
                     /locus_tag="VFQ23_12265"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HET9907417.1"
                     /translation="MANNRSTKAKAGPTPEEPPEEPVWTYRGYRLRTSEFVTAMVHFF
                     RAEVQRANVWRQRLDTTTNWAVVATGATLSVAFSQPEIHHGIIILNTLLVMWFLFIEA
                     RRYRYYELWSYRVRLMETDFYAAMLVPPFHPSPEWAESLAENLLMPHFPISMWEALGR
                     RLRRNYFWIFLILYASWVAKIWLFPDPAPTWAVFIERSAVGPLSGQFMFVLGLLFYTS
                     LALLAIFTITMTRASGEVLPRFGSDDTFFHSVPHGAHQPSTGLRSWLASRQRRRKFLA
                     LIITGSPKEVSTRILSDLGRGVTAIDGRGMYTGADRTILMCALTVTEVHNLKTAVAKE
                     DPRALVIVSPTQEILGGGFDPLAEETKA"
     gene            complement(2355..2999)
                     /locus_tag="VFQ23_12270"
     CDS             complement(2355..2999)
                     /locus_tag="VFQ23_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907418.1"
                     /translation="MLNITKKIGTCIITSVYIASCQVIGPVSNAQLAQTLVSNPNYLD
                     QCKSLNPIKLSEQISYKNVWPGKTKASEVESILGTPQEKSSFDGGTNLVYGKSEFFVS
                     VENGLVTYIVVDPNPETNLSITLEEVILRHGCPDVILAFNTTEDQSGYNSVRLIYSTI
                     GMEISFANYPTSLGSSALNTSYFPPMTVQEFFDTYSWTEMRFFTQPVEWSEAIK"
     gene            3635..5740
                     /locus_tag="VFQ23_12275"
     CDS             3635..5740
                     /locus_tag="VFQ23_12275"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9907419.1"
                     /translation="MNRLSTVTSPYLLQHAYNPVDWFPWGEEALNKARKENKPIFLSI
                     GYAACHWCHVMAHESFEDNEVAAFMNEHFINIKVDREERPDLDGIYMQATMAMTGSGG
                     WPMSVFLTPDLKPFYAGTYFPPVRRYSMPAFRDVLAGIARTWKEDRAEVDRVSTQLSD
                     HLQQALNINIKTEATFSQEQLDAAAKSLVDSYDWENGGWGAAPKFPQPMAIEFLLRRA
                     ATNRAERRESEDEASLTPTPSPTERGELKVAIHALKAMARGGMYDVVGGGFSRYSVDQ
                     FWRVPHFEKMLYDNAQLVRAYLHAWQITGDDFFKRIVVETLDFIEREMTHPDGGFYSS
                     LDADSEGEEGKYYVWTLEEIREVLAEDSEFFEAAYGITARGNWEGKTVLQRALDDASL
                     STRFKPDLETVPEKLAELHSKLLTARAVRIRPGTDDKILTSWNGLMLATMSEAARVLS
                     LEAGRLQNKYYILATRNAEFLLNALRPDGRLKRSWRAEKVTNEVFLEDYAGLVLGLLE
                     LYQTDFNTKWFVSAKELADEMIELFSDSNGGFFDTSKDSEEILLRPKDLQDNATPSGN
                     ALACEALLKLAAFTDDGNYRDLAEKSLGLSAVMAAQYPTAFSRWLSAADFALSNVKQV
                     AMVYESEDGQATELIQLVEAAYRPNVIVAASAYPPSNTAPPLLMDRPLKDGRATVYVC
                     EGFVCKNPINTISELKELL"
BASE COUNT         1489 a         1411 c         1416 g         1432 t
ORIGIN      
        1 tcatggctga tcaattttca ttacgtgttt attcatatct tgaccgcatg caaccgcaat
       61 acgcggcatt cgtcggcacc atcacgcaag gtgatcttcc caccgagggc atggcgtctc
      121 tgtatgtaga ggtggcaccg ggcaacgagg tattccgcct tgtcgatatc gcggtgaaat
      181 ctacagaagc caagcctggc gcacagctag tggagcgcga gtttggtatg ttcgaagttc
      241 actcgcggtc acagtccgaa gtgttggaag ccgggcgtat cgtgctggac cgtctcggtt
      301 tgaagcccga agaacggatc aaaccgttca ttgcctcggt acagatcatt accaacgtgg
      361 atccatatca ggctcagtta ttgaaccgct tccgccgcgg ctcgatgctg gtcccgggtg
      421 agacgatgct cgtgctcgaa tgcgctcctg ccgcctatat caacttcgcc tgtaatgagg
      481 ctgaaaaggg cgcagcgatc aagattctac atgtgtcgtc cgtgggacgt tttggacgca
      541 tgtggttatc cggttcggag gcagaaattc tgacagcgcg caactcggca gtgaaggcgc
      601 tcgaagggtt agatggaaga ccagagaaat gacctgccac tgagtcacgg agtcgcaaag
      661 ccttttgatt ctccgtttct cagcgtcgtt atgatcccca ctcggggact gtggcaagtc
      721 ttaaggatac cttatgccaa agatcttcta tactgaacgc gacattgatg acttgaaagc
      781 ccgcggtatc accagtctgg atgtgactga taatattgtc ttaacggatc tggcagtcga
      841 acgcgccatg aagcatgaga tcaaggtaaa tcgtgtggaa cactccgcgc caatggcatc
      901 ctatagtccg tccgttaata cgtacgcggc gtatccaaga gagacaaata attctgtcaa
      961 cgcggaacta aaggaaaaaa tcaaagccgc ggtcctcgca cgtctaaacg gacaagtgga
     1021 cgcggcgctg ctcgatgcag tcatcaccaa agtactggca ggaatgaaat aattaaggta
     1081 tatacttaac gagcatcgcc agcgcgtgag ctcgagcacc ttgactagaa tactttattt
     1141 gaacgtgacc atattaatcg ggggaaacga tagccataat tcattatgca gcgacactgg
     1201 caaatgccgc gttggcaatc ccatagcatg tgaggcataa acgtatatgg caaacaatcg
     1261 atcaacaaaa gcaaaagctg gacccacacc agaagaaccg cctgaggagc ccgtctggac
     1321 ctatcgaggg tatcggctca ggaccagtga atttgtcacc gcaatggtgc atttcttccg
     1381 cgccgaagtc cagcgagcga acgtctggcg tcagcgactg gataccacca ccaattgggc
     1441 tgtggttgcc actggcgcaa cgttgtcagt ggcctttagc cagcccgaaa ttcatcacgg
     1501 aattatcatc ctcaatacat tgttagtgat gtggtttctc tttatcgagg cacgccgata
     1561 tcgttattac gaactctgga gttaccgcgt ccgactcatg gaaactgact tctatgcagc
     1621 gatgcttgtc ccgccattcc atccatcgcc tgaatgggcg gaaagcctgg cagaaaattt
     1681 gctcatgcct catttcccca tctcgatgtg ggaagcatta ggtcgccgtc tgcgccgcaa
     1741 ttatttctgg atcttcctga tcctttatgc atcctgggtt gctaaaatct ggctctttcc
     1801 ggacccagct cccacctggg cggtatttat cgaaagaagc gcggttggtc ctctatctgg
     1861 tcagttcatg tttgtgctgg gactgttgtt ctacacatcc ctggctttgc tggcaatctt
     1921 taccattacg atgacccgcg cctcgggtga agtgctgccg cgctttggga gcgacgatac
     1981 gttcttccac tccgttccgc acggggcaca tcaaccctca accggattac gctcctggct
     2041 cgcctcaaga cagcgcaggc ggaagtttct ggcgctgatc atcaccggca gcccaaaaga
     2101 ggtctctacc cgcatcctct cggacctggg gcgaggagtc accgccattg acggacgcgg
     2161 catgtacacc ggcgcggacc gaacgatcct catgtgcgcg ctgaccgtga ccgaggtaca
     2221 caacctcaaa accgcggtcg caaaagaaga cccgcgcgcg ctggtcattg tttcacctac
     2281 acaggagatt ttaggtggcg ggttcgatcc gttagcggag gaaacgaagg cataaaattc
     2341 gatgcgtgct caaattattt tatcgcctcg ctccactcaa caggttgggt aaagaatcgc
     2401 atctcggtcc aactgtatgt gtcaaaaaat tcttgaactg tcatcggagg gaagtatgag
     2461 gtgttcaagg cactgctgcc caggctagtt ggataatttg caaaactgat ttccatgcct
     2521 attgtgctgt aaatgaggcg aacactgttg tagccagatt ggtcttccgt tgtattaaac
     2581 gccagtatga catcgggaca accatgccgg agaataactt cttcaagcgt tattgataaa
     2641 ttggtttctg gatttgggtc cacaacaatg tacgtaacta gaccattttc aacgctcaca
     2701 aagaattcag atttgccgta caccaaattt gtaccaccat caaatgaaga tttttcttga
     2761 ggtgttccca aaatggactc gacttcagat gcttttgttt tgcctggcca aacatttttg
     2821 taggaaattt gttctgataa ttttatagga tttagagatt tacactgatc taaatagttt
     2881 ggattcgaaa ccaatgtttg agctaattga gcatttgaaa caggtccaat tacctgacag
     2941 gaagctatat atacagatgt aattatgcat gttccgattt tcttagtaat gtttagcatt
     3001 tgaagttcct cttcatcaaa gaaattctag caagcgcaga aagatcttgg accaatcagg
     3061 agattttaga gggaggtttt ggctggcaga gaaaggcaaa tgtaactgac tggcagtgac
     3121 aagcgatccg ctagtttcgt ggcgcgcctt gttgtacgtc atggtttctt cttgttacaa
     3181 gtaacccaat ccagatcgct aatgaataca tgctagttaa ggcaagaaaa aatacatcct
     3241 gtggtcgcgg ccagaatatt cctagcatta gtattgcata taggatatag ctgggtaatt
     3301 tactaacttt agaacctgag gcatctggtt taacatggtt tctcttatat tcaatcagaa
     3361 atgtaattat ccccaggagt gctccaatac ctacggctaa taggtaattt aaactagcat
     3421 acctttcttc aatcagggtt aatatgagac ccaccagaaa catcaagaga taaaaattcg
     3481 ccggtgcaaa aatattctta aacttttgca tgatttaccc aagtcactgt gatacgctaa
     3541 ttcagtgaat accgcagtcg cgagaacaga agaggaaggg aatagtcgtg gataagcatt
     3601 gattggtatt ccacgcatta taattacttt acccatgaac aggctatcaa ctgtaacctc
     3661 cccttactta ctccagcacg cctacaaccc cgtggattgg tttccgtggg gcgaagaggc
     3721 tttgaataaa gcacgtaaag aaaacaaacc gattttctta tcaataggct acgccgcatg
     3781 tcactggtgt catgtaatgg ctcacgagtc ttttgaagat aacgaagtcg ccgcgttcat
     3841 gaatgagcac tttatcaaca tcaaagtgga tcgcgaagaa cgccccgacc tggatggcat
     3901 ctacatgcaa gccaccatgg caatgaccgg ctcaggcggc tggcctatgt ccgtttttct
     3961 cacgcctgat ctcaaaccat tttatgccgg cacctacttc ccacccgttc gtcgctacag
     4021 catgccagcc tttagagatg tactcgcagg catcgcgcgc acatggaagg aagatcgcgc
     4081 cgaagtagac cgcgtcagta cacagctctc tgatcatttg cagcaagcct tgaacataaa
     4141 catcaaaaca gaagcgacat tttcccaaga gcagcttgat gccgctgcaa aatctctggt
     4201 agattcctat gattgggaaa atggcggctg gggcgccgcg ccgaaatttc cgcagccaat
     4261 ggcaatcgag tttttgttac ggcgggcggc tacaaatcgc gctgagcggc gcgaaagcga
     4321 agacgaagcg tccctcaccc ctaccccctc tcccaccgag agaggggaac ttaaagtcgc
     4381 catccacgcc ctcaaagcga tggcgcgcgg tggcatgtac gatgtagtag gcggtggctt
     4441 ctcgcgttac agtgtcgatc aattctggcg cgtcccccat ttcgagaaga tgctttacga
     4501 taatgcccag cttgtacgag cttatctcca tgcctggcag atcaccggcg acgatttttt
     4561 caagcgcatc gtcgttgaaa ccctggattt catcgaacgc gaaatgacac atccggatgg
     4621 tgggttttac tccagcctcg acgccgattc agaaggggaa gaaggtaagt attacgtttg
     4681 gacgttagaa gaaattcgag aagtacttgc agaagattct gaattcttcg aagcggctta
     4741 tggaatcacc gcacgcggaa actgggaggg caaaacggtc cttcaacgtg cgctggatga
     4801 tgcatctctc tccactcgtt tcaagcctga cctcgagact gtccccgaaa aactcgctga
     4861 actgcattct aaactattaa ccgcgcgcgc cgtgcgcatc cgccccggca cagatgacaa
     4921 gatcttaaca tcctggaatg gactcatgct cgcgaccatg tctgaggcgg cacgtgtctt
     4981 atcgttagag gctggcagac tccaaaacaa atactacata ttggctacac gtaacgcaga
     5041 gtttttattg aatgcgctca gacccgatgg gagacttaag cgaagttggc gtgcagagaa
     5101 agtaaccaac gaggttttcc ttgaagatta tgctgggctc gttcttggct tgctcgagtt
     5161 gtatcaaacc gacttcaata ccaaatggtt tgtgtctgcc aaagaacttg ctgatgaaat
     5221 gatcgagctg ttcagtgatt ccaatggcgg cttcttcgat acgtcaaagg atagtgaaga
     5281 gatcttactg cgcccgaagg atttacagga caatgccacc ccatccggga acgctctggc
     5341 gtgtgaagcg ttactaaagc tcgcggcatt cacagatgat ggaaactatc gcgatctggc
     5401 agaaaagtcg cttgggcttt cggcagtcat ggctgcacaa taccctactg ctttctcaag
     5461 gtggctatcc gcagcggatt ttgctctgag caatgtgaag caggttgcaa tggtgtatga
     5521 atcggaggat ggtcaagcaa ctgagttgat ccagttggtc gaggctgcat atcgaccgaa
     5581 tgtgatcgtt gccgcatccg cttatccccc atcaaatacg gcaccgccgc tgttgatgga
     5641 tcgcccacta aaggacggaa gagcaacggt gtatgtgtgt gaaggtttcg tttgtaaaaa
     5701 tccgattaat acaatttcag aactaaagga gttgctgtaa aatcagac
//
LOCUS       DASTJP010000424        11304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_437231, whole genome shotgun sequence.
ACCESSION   DASTJP010000424 DASTJP010000000
VERSION     DASTJP010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437231"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFQ23_12280"
     CDS             <1..184
                     /locus_tag="VFQ23_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907420.1"
                     /translation="TLVWQNGAVVGSTSVACEGHGLQVTVSYDHQVFMPFMSQFIGGV
                     VPLNATVTDTILSPLC"
     gene            381..1607
                     /locus_tag="VFQ23_12285"
     CDS             381..1607
                     /locus_tag="VFQ23_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET9907421.1"
                     /translation="MHNLLQRRGQALIVVALALAGLIGIVGLVVDGGNAFLDRRKAQN
                     AADAAALAGALARIRGEDMITTAFASAAQNGYNNDSVTNRVEFFSPPKDGPHSDNVEY
                     IQIIITSHIDTYFARIVGRSQITNVVQAVARTKGSEYKQLLSGMAVVSLAPSSNCGNQ
                     KSFWIHGNATLDISGGGVFINSNNETCALLQHGNGSLRVNDGYRIDVVGGASIQKPLL
                     FTPGVVVGAVPVNYPPPFFMPEVDCEGETAQVSADGTSMTPGIWKDEFPPPGVMHLES
                     GNFCLENGMNINGPLQGSDVVFKVMDGDVRFGTANIILDAPDSGDNAGLLIYLPQENN
                     NRVVLDGGSGSIISGTILAPASPIVINGNSSSSGFHSQIIGYTVEAGGSGDVVIVYND
                     AENFNSHTMPEIQLSE"
     gene            2094..3935
                     /gene="glmS"
                     /locus_tag="VFQ23_12290"
     CDS             2094..3935
                     /gene="glmS"
                     /locus_tag="VFQ23_12290"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET9907422.1"
                     /translation="MCGIVGYIGPRDATPIIINGLKRLEYRGYDSAGVAVLNEDQLEV
                     RKDAGKLSQLIDLVGKSPINGAPGIGHTRWATHGAPNARNAHPHLGQTKRVVVVQNGI
                     VENFLELKDELTAEGVVFQSETDTETIVHLVEHYLATGIGLVEAARKTFNEIQGANVV
                     VLMSADEPDKIVTARIGNAGGVVLGLGEGENYIASDTPAIMEHTRNVIFLESRQMAVV
                     TKSTITVETLDGAEVKPHVHTVSWDPVAAEKGEYRHFMQKEIHEQVRALTDTLAGRVD
                     FANGKIRLPDLNLTEELAKKIEKIYITACGTAAYAGMVGKYLIEKIARVPVEVVIASE
                     FRYSDPILNDRTVVLAISQSGETADTLAAIEEGRRKGAIIWSIVNAIGSQAMRVADGF
                     ISMQTGPEIGVASTKAFTAPLVDQYMLAILLADLRGTIDDKTRNKLVSDLRLIPDLVG
                     RTLERDCEVEAVAHALKDIRDCLYLGRGINMPIAYEGALKLKEISYIHAEGYPAGEMK
                     HGPIALIDEHMPVLCLAPKDPWHEKMISQIQQAKARDGVVIAVATDGDELIEGMADHV
                     LWVPETPWMLSPVITVLPLQLLAYHIAAIRGLDVDQPRNLAKSVTVE"
     gene            4147..5379
                     /locus_tag="VFQ23_12295"
     CDS             4147..5379
                     /locus_tag="VFQ23_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HET9907423.1"
                     /translation="MKINSLEKGQALIVIALAAVVLFGFAALAIDGSMVFSDRRHAQN
                     AADTAALDAALSKTRGGSWQQEGLDRATSNGYNDNGTSNEVEVYSPPIDGNYAGNSEY
                     IQVKITSHVNTVFARVIGYQQLTNKVEAVARSVPGTSSPMFDGHAVVGLAPHECKAIM
                     YQGNANTTVEGGGIFVNSDCATSAFFNNSSSAQLTAPCLNAVGGINYKPGAIDIPGCI
                     NSGASPFNYPPDNMVFPNIVCPSGESQNGTTLNPGTYTSGNKFPPNGVTHLNGGTYCV
                     HNDFSVQGGDTLIGHNVIIIMVEGDVTFNGGATIELSGPAGPQSAENPYGGLFLYMPM
                     SNSGTININGNSESGFTGTILAPAANISINGTGDNGLHGQIIGYTVDLSGTSDTNIVY
                     DNDENWDAPVPPQIELSQ"
     gene            5652..7097
                     /locus_tag="VFQ23_12300"
     CDS             5652..7097
                     /locus_tag="VFQ23_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HET9907424.1"
                     /translation="MFYKKSEKGQALVLIALAAVGLFGFAALSIDGGMVFSDRRHAQN
                     AADTAALAAALARVRAASNPDAAAIAAGEARAITNGYGDPNDPDSTVEVNFCNETGII
                     CEGLPAGADPADYIRVKITSIVRLTFARIIGRQTLTNILTAVAKAEVGGLAPLFDGSA
                     LVALAPTGPETVSGQGNVWLTINNSGVFDNSNSDCAMSEVGNITYWVETAYNVVGTHC
                     PASNPNSSYTGPVQQGAEQVEYPPDIDIPTPSITCSGNAPAPTQDATPGHFTIHPGNY
                     PSGLTINGGTYKFAGNGKFCFHGNVNVNGNAKVIANYADILLTSGSITFNANSTFTCS
                     YVLVHIDGGSGMHFNGNASNVCDGVTFFAETGSVTWNGNVSNTFKAPYESPTHPLGSY
                     PEYQNVLIYMPYGNTSPLTINGNAGNQLTGSIIAVSSDVTISGNSGTTGLNSSITGYT
                     ITLAGNSNTTINYVPEDQFQQVDPSSLGLTK"
     gene            7369..9756
                     /locus_tag="VFQ23_12305"
     CDS             7369..9756
                     /locus_tag="VFQ23_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9907425.1"
                     /translation="MKGQLHLRSFFLSLTIIAVLLFSAMGTTAAYADDGTKTDPAPTE
                     ETTGGGATDEASAESASSESAEGESKDSQTDVTTEVSVDSSEVAAVEEVTTAESATGA
                     ATVDTPILEQVPENTTVAVLDAQGQPQPLATAASADAIAVTSDPIWCPAGQAPTPGAN
                     GCTASFTSFDALLTELAGNATYTGAGTIYVQQGAYAGGESTIDFNSYNLSNISGSDLT
                     VQGGWNTSTGDTSSTSTFNISLILGSSANPWGGSVTLNNLSFNGTNGTGITVYSQANI
                     NLTNVQSSASVTGSGAELTAGGDVNINDSSFDRNREAGAIVNAGGNVSVANSSFSNPT
                     SARLQMTGLDITSGGNVSLLDVIADGNREVGAIIDAGGRVSIFGTIDLVNGTSTTSFS
                     GTKSSTTTTCPGSPSQFCGFGLQVTTPGSIDLQGVIGNDNFLWGANLNAGQDVNIVDS
                     IFNANSTEVPTFIDDTGIFITAGGNVSLNNVTANDNRLFGASIDAVGNVSVNNSNFLS
                     NNGVTVSGSGATQYDGIGLQVNSDSGIFINNTNASGNSLFGADLTAGGDVAIANSIFS
                     NTTTGDTAAPLGMGLQIVSGQNVFIDTVTLDGNQVDGASIQATGDVFLDFITATNNGA
                     NGVEVDAACTNLNSGTYSGNGEYGLSLVNPTLNQLVPPVFSGNGLGDINPAAPGACPP
                     PVFPVVDGGTTNGGATGGVSTLSGSISNRNASFVSMSNSSYKNLGLINSGALTLNNLF
                     AGNYQLLGVSSTSNGNVTALGIFTGKYAYVHSAQGLQIVFLTQPAIFSGLWSNES"
     gene            9837..10385
                     /locus_tag="VFQ23_12310"
     CDS             9837..10385
                     /locus_tag="VFQ23_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907426.1"
                     /translation="MKKFMLLLLPSILLVGMLISGCGNVQTAIPTNTQEALPSPSTST
                     PTITFTPDPCGTENIQAEVDKVHRHMREFDDASILASSTPRDQLSTAIADLQRIRRAA
                     EDEVTASCLGDLKRYQIQHMNLVIDTLIAFLSGTEQAVLDQGIAAARQQHDQYTLELA
                     RILGITVVPATPAVVPSPTPTP"
     gene            complement(10454..10651)
                     /locus_tag="VFQ23_12315"
     CDS             complement(10454..10651)
                     /locus_tag="VFQ23_12315"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET9907427.1"
                     /translation="MTTQQKRSLRRSRSNSMIAGVCGGLAEFFGISAFWFRLGFIIAF
                     IPGGVPGVLVYLLMWIMIPKE"
     gene            complement(10714..>11304)
                     /locus_tag="VFQ23_12320"
     CDS             complement(10714..>11304)
                     /locus_tag="VFQ23_12320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9907428.1"
                     /translation="CHENRGLTPHIEDVTRRFAKAGYVGLAVDLLSRQGGSTVVGEGN
                     VPGALGNILPDQFVEDFKSGWQYLTNLSFADTARVGMTGFCFGGGVTWRVATQMSELL
                     AAIPFYGPPPPVEDVPNINAAVLAIYGELDSRINGSIPQIEEAMAANGKIYAKMIYPN
                     ADHAFHNDTGTRFNAEAAQDAWQKTLDWFDQYVRNA"
BASE COUNT         3052 a         2679 c         2720 g         2853 t
ORIGIN      
        1 cacattagtt tggcaaaatg gtgcagttgt aggctcaacc agtgttgcct gtgaaggtca
       61 tggacttcaa gtaaccgttt catacgatca ccaagttttt atgccattca tgtctcaatt
      121 cattggtgga gtagtcccgc tcaacgctac agtaacagat acgatactat cgccgctttg
      181 ttaatatcca ttcttatatt agtctttgtt cggaattaaa ttcaattggt aactttacct
      241 ttgacaacta ttggtccaag atgtggattt atgtgttgga ttctgcactc aagactttca
      301 tgcatcactg aacacataag ttaacaaacg ttcatttaga tatatgggga tatgaagtag
      361 aataattgca ggagacgatc atgcacaatc ttttacagag acgtggtcaa gctttaatcg
      421 tggtcgcgct tgccctcgcg ggcttgatag gtatcgttgg cttggtcgtt gacggcggta
      481 atgcattcct ggaccgtcgc aaagcgcaaa atgcagcaga tgctgcagcc cttgctgggg
      541 cattggctcg catccggggg gaagatatga tcactacggc gtttgcatct gctgcccaga
      601 acggctataa caatgatagt gtaacaaatc gtgttgagtt ctttagtcct cctaaagacg
      661 ggccccattc agacaatgta gaatatattc agatcattat cacctcacat atcgatacgt
      721 actttgcgcg tattgtaggc cgcagtcaaa ttaccaacgt tgtccaggct gtggcacgca
      781 cgaaaggatc tgaatacaaa caattattaa gtggtatggc ggttgtcagt cttgcaccgt
      841 ccagcaattg tgggaaccag aagtcttttt ggattcatgg aaatgccaca ctggatatca
      901 gcggcggtgg tgtttttatt aactcgaaca acgagacgtg tgcactttta cagcatggaa
      961 acggcagcct tcgcgtcaat gatggatatc ggatcgacgt tgtaggcggt gcatctatcc
     1021 aaaaacctct ccttttcaca ccaggggttg tggtgggtgc ggtcccagtc aattatccac
     1081 cacctttctt tatgccagag gttgactgcg agggagaaac tgctcaagtg agcgcagatg
     1141 gaacatccat gacacccgga atatggaagg atgaatttcc accaccgggc gttatgcatt
     1201 tagaatctgg caatttctgt cttgaaaacg gcatgaatat caatggtccg cttcagggat
     1261 ctgatgttgt ctttaaagtg atggatggag acgtccgctt cggtacggcg aatattattc
     1321 tggatgcccc cgacagcgga gataatgccg gcctgctgat ctatctgcca caagaaaata
     1381 ataaccgggt tgtactcgat ggtggatcag gatctatcat cagcggaact attttggcac
     1441 ctgcctcacc catcgtgatt aacggaaata gttcttcaag tggattccat agccaaatta
     1501 ttggctacac agtcgaagcc ggtggaagtg gagacgtggt aattgtttat aacgacgcgg
     1561 aaaactttaa ctcgcatacc atgcccgaaa ttcaattgtc agagtagaag cgctcgaatt
     1621 tgatacgttt ctcttaggac tactgagacc agaaaatatg atttagagcg taacaataat
     1681 tgcaagattc cgtcatatta acggtctcga tagattatct gactgaaaat attcgccatg
     1741 cacaatatga aaggacggtg atgctaacaa caaaataatt gaggaaagac agcgcatgat
     1801 cctgaagcct gccgcatgcg gcaggttgag gatgacgagg acatgggttc tccatcgcag
     1861 gatggagtta acctcggcaa aatttgccga gggaaaatcg acaggtcagc ggaagcccat
     1921 cgggtgcgcc atcacccgtt ttatctttcc ctccgaacat aaaccttgca acaatacctc
     1981 atgagcaatt gtgtgggcaa agctgcaaag aatggtacac gaaattggag aatagaattc
     2041 cattagtttc tgctctctaa ttatcaaatt tctaatacta tcggaggatc cccatgtgtg
     2101 gaatcgtagg atatatcggg ccgcgtgacg cgacaccgat tattatcaat ggcttgaaaa
     2161 ggctggaata tcgcggctat gactcagctg gagtcgctgt attaaacgaa gatcagcttg
     2221 aagttcgaaa agatgcgggg aaactatctc aattgatcga ccttgttgga aaatcaccta
     2281 tcaatggtgc gccagggatt gggcatacac gttgggctac gcatggagca ccgaacgccc
     2341 gcaacgccca tccgcatctc gggcaaacca aacgcgtcgt tgtcgtgcag aatgggattg
     2401 ttgaaaactt ccttgagcta aaagatgaac taacagcgga aggtgtggtt tttcaatccg
     2461 agactgacac cgaaacgata gtccatcttg ttgagcatta tctggcaacc ggcattggct
     2521 tagtggaagc tgcaagaaag acattcaatg aaatacaggg agcaaatgtt gttgtgctca
     2581 tgtctgcaga tgagcccgat aaaattgtaa ccgcacgcat tggtaacgca ggcggtgtag
     2641 ttttgggtct gggggaaggc gaaaattaca ttgccagcga tacaccagca atcatggaac
     2701 atacgcgcaa tgtaatcttt ttagagtcaa ggcaaatggc tgtggtaacg aaaagtacaa
     2761 ttacggtcga gacactggat ggagccgaag tcaagccgca tgtacatacg gtttcgtggg
     2821 atccggtcgc tgctgaaaaa ggtgaatatc gccacttcat gcagaaggaa atccatgagc
     2881 aagtgcgggc gctgacagac acattggcag gccgcgtgga ctttgcaaat ggaaaaatcc
     2941 gtctaccgga tctaaattta accgaagagc tagcgaagaa aatcgaaaaa atttacatca
     3001 cggcctgcgg gacagctgcg tatgcaggaa tggtcggaaa atatttaata gagaaaatcg
     3061 ccagagtgcc agtcgaagtc gtcattgcct ccgagtttcg ctacagtgat cccatcttga
     3121 acgatcgaac agtggtattg gccatttcac aatccggtga aacagcagac actctggcgg
     3181 cgattgagga aggacgtcgt aagggtgcaa tcatctggag catcgtaaac gcaatcggtt
     3241 cacaggcgat gcgcgtggca gatggattca tttccatgca aaccggaccc gagatcggcg
     3301 tcgcttcgac aaaggctttc accgccccgc tcgttgacca gtatatgctg gcaatcttac
     3361 ttgctgactt gcgcggtacc atcgatgata agacacgtaa caaactggtt tccgatctgc
     3421 gtcttatccc tgatcttgtt ggccgaacgc ttgaacgaga ttgcgaagtg gaggcggtgg
     3481 cacatgcatt gaaagacatt cgtgactgcc tttatttggg tcgcggtatc aatatgccca
     3541 tcgcctatga gggagcattg aagctaaaag aaatttcata tattcacgcc gaagggtacc
     3601 cggcaggtga aatgaaacac ggtccgatcg ccttaatcga tgagcatatg cccgtgttat
     3661 gtcttgcgcc gaaagatccc tggcatgaga aaatgatttc acagatacaa caggccaagg
     3721 cgcgcgatgg cgtagtcatt gccgtagcca ctgacggaga tgaacttata gaaggaatgg
     3781 cagatcatgt gttatgggtg cctgaaacac catggatgtt atctcctgtc attactgtgt
     3841 tgcctttgca attgctggca taccacattg cggcgattcg agggctcgat gtggatcagc
     3901 ccaggaacct cgcaaaatct gtcactgtag aataatgaat atcaataacc taaagtaaac
     3961 ggtaccgaat tctcaattag gttacagaga gacgatgaga ggcgcacagg tgtgcgcctc
     4021 tcatgtttta aagctttcaa taaagcaaat ctgtcagaga cctatcatgt ttgtatggcg
     4081 atttgatgta tcaaagaaga agggggcttc gtcatgcgtg cagtcactga aaaaggagtt
     4141 gacgctatga aaattaattc tttagaaaaa gggcaggcgc taatcgttat tgcgttggca
     4201 gcagtcgtac tattcggttt tgctgcgctt gcgattgacg gtagtatggt attttcggat
     4261 agacgccatg cacagaacgc agcagataca gctgccctag acgcagctct ttcaaagaca
     4321 cgcggcggat cctggcaaca ggaagggcta gaccgtgcaa ccagtaatgg ctataacgac
     4381 aatgggacat cgaatgaagt tgaagtctat agcccaccga ttgatggaaa ctacgctggt
     4441 aatagtgaat acattcaagt caagatcacg tcccatgtaa atactgtttt tgcacgtgtg
     4501 attggctacc aacaactaac gaataaggtg gaagccgtgg cccgttctgt tccggggaca
     4561 agctctccaa tgttcgatgg gcatgccgtt gttggtcttg cacctcatga gtgcaaagca
     4621 atcatgtatc aggggaatgc caatactacg gtcgagggcg gcggaatctt cgtcaactca
     4681 gattgtgcaa cttcggcttt tttcaataat agttcatccg ctcagttaac ggcaccatgt
     4741 ctgaatgcgg ttggaggtat caactacaag ccgggagcca ttgatattcc aggctgtatc
     4801 aatagcggtg cgtcaccatt taactatcca ccagataata tggttttccc caatattgtc
     4861 tgtccttccg gcgaatcaca aaatggcact accctcaacc ccggaactta cacctctggc
     4921 aacaaatttc cgccaaatgg tgtaactcat ctcaatggtg gtacctattg tgtacataac
     4981 gacttcagcg tccagggtgg agatacgttg attggacaca atgtgattat catcatggtg
     5041 gaaggcgatg ttacatttaa tggtggagca accattgagc tcagcggacc cgcggggcca
     5101 caatccgcag aaaacccgta tggtgggctt ttcctgtata tgccaatgtc taactccgga
     5161 accataaata tcaatggcaa ttctgaatcg gggttcactg gcaccatact ggcgccggct
     5221 gctaatatct caattaacgg tactggtgat aacggccttc acggccagat catcggatat
     5281 accgtggatc tttctggaac ttcagacaca aacatcgttt atgataatga tgaaaactgg
     5341 gatgctccgg tgccccctca aattgagcta tcacaataaa catagcgaaa atatgggtca
     5401 tacagcgaaa aagcaacgtc aaaagcgttg cttttttgat tcgaaggaat cctaccttga
     5461 gtcttagata gcacataaaa tcggcttgcc tttgagatgg atcactcacg tgaagttaca
     5521 gtaggtcatc ccccactagt actaaatctt ggtacggttt tcacaattgt atattgcact
     5581 ttcgaaaaga aaccatataa taaagacaga cttacgagat acctgtttta gatgaaagta
     5641 aggagagtag aatgttctac aaaaaatctg aaaaaggaca agcgctcgtc ctcattgcat
     5701 tggctgcagt ggggcttttt ggttttgcgg cgctttcgat tgatggtggt atggtgtttt
     5761 ctgataggcg tcatgcccaa aatgctgccg ataccgctgc ccttgcagct gcacttgcaa
     5821 gggtaagagc tgcttccaat ccggacgctg ctgctattgc tgctggtgag gctcgtgcca
     5881 tcacgaatgg ttatggtgat ccgaacgatc ccgactccac cgtcgaagtt aatttctgta
     5941 atgagacagg tatcatctgc gaaggtttgc ctgccggcgc cgaccccgct gattatataa
     6001 gggtaaagat tacgtcaatc gtgcgcctta ccttcgcacg gattataggc agacaaaccc
     6061 taacaaatat tcttacagcc gttgcgaaag ctgaggttgg cgggttggcc ccattattcg
     6121 atggatcagc cttagttgcc ctcgcaccaa ccggtcctga aaccgtcagc ggacagggaa
     6181 acgtctggct cacgattaat aacagtggcg tttttgataa ctcaaatagc gattgtgcca
     6241 tgagtgaagt tggtaacatc acttactggg tggagactgc ttacaacgtt gttggtacac
     6301 attgtcctgc ttcaaatccc aattcctcgt atacgggtcc agtgcagcaa ggtgccgaac
     6361 aagttgaata tcccccagac attgatattc caacaccttc cataacttgt tctggtaatg
     6421 cgccggcccc tacgcaggat gcaacgcctg gtcactttac gattcatcct ggaaattatc
     6481 cttccggact caccatcaat ggtggaacgt ataaatttgc tgggaacggt aagttttgct
     6541 tccacgggaa tgtaaatgtc aatggtaatg ccaaggtaat agcgaattac gcggatatcc
     6601 ttctcacttc gggaagcatc actttcaacg cgaacagcac cttcacttgt tcctatgtgc
     6661 tggttcacat tgatggcggt tcaggaatgc atttcaatgg aaatgccagt aatgtatgcg
     6721 atggtgtgac tttctttgca gaaaccggca gtgtgacctg gaatggtaat gtatccaata
     6781 cctttaaggc gccctatgaa agccccactc acccactggg ctcctaccca gaatatcaga
     6841 acgtgctgat ctatatgccc tatggcaata cttctcctct caccatcaat gggaatgccg
     6901 gcaatcagct cacgggctcg atcattgccg tttcttctga tgtgaccatc tctggcaaca
     6961 gtggaacgac aggactcaac agttcaatca ccgggtatac gattacactg gctggtaaca
     7021 gcaacaccac tattaactat gtacccgaag atcagttcca acaagtggat cccagttcgc
     7081 tgggattaac caagtaacaa tttcagaatg accatgagac cgtgtatgca cacggtctct
     7141 aatttttttt attatgttcg agtaggaggt ggccaacaat ccttatctca tcgcagaact
     7201 aagattctaa tgatagtaca catccgatgt gtaacctttt gtaatctttg caagttgact
     7261 tcgataaggg caattcttat aataaaatca acacgaatgc tattgcgttc gtgcattaga
     7321 tgatttatgg ggtagtaccg caaataatat tgttacgagg agagtattat gaaaggtcaa
     7381 ttgcatttac gttcattttt tctttcattg acaatcattg ctgttttact ttttagcgcg
     7441 atgggaacca ctgctgcata cgccgatgat ggtacaaaaa ctgacccagc ccctaccgag
     7501 gagacaacag gtggaggcgc cacagacgaa gcatccgcgg agagtgccag ttcagaaagt
     7561 gctgaaggag aatccaaaga ttcgcaaaca gatgtcacaa ctgaagtatc tgtcgattct
     7621 tccgaagtgg ctgctgtcga agaagtgacg actgcagaaa gtgcgaccgg agcagccaca
     7681 gtggatacac cgatccttga gcaggttccc gaaaatacca cggtcgcagt tttggatgcc
     7741 caggggcaac cccaaccatt agccaccgca gcttccgcag acgcgatcgc tgtgaccagt
     7801 gacccgatct ggtgtccagc cggccaggca ccgactccag gcgcgaatgg atgtacagcg
     7861 tcttttacaa gttttgatgc cttactaact gaactggcgg gcaacgcgac atatacgggc
     7921 gcaggcacca tttatgttca acaaggcgct tatgctggcg gtgagtcaac gatcgacttt
     7981 aattcatata acctgagcaa tatcagcggc tcggatctga ccgtgcaggg tggttggaac
     8041 acaagcactg gagacacttc cagtacttcc acgttcaata tctcccttat ccttggctca
     8101 agcgccaatc cctggggcgg ctcagtgacg ctcaacaacc tgagtttcaa tggcacgaac
     8161 ggaactggca ttacagtgta ttcacaggct aacataaacc tcaccaatgt tcagtcttca
     8221 gcttccgtca caggctcagg cgcagaactc acagcgggtg gcgatgtaaa catcaatgac
     8281 tcaagttttg atcgcaaccg agaagcaggt gcaattgtta atgcgggcgg caacgtctct
     8341 gtggccaaca gcagcttcag caatcctact tcagcccgtc ttcagatgac agggttagat
     8401 atcaccagcg gcggaaatgt ttccttactg gatgtgattg ccgatggaaa tcgtgaagtc
     8461 ggtgcaatca tcgacgcagg tggcagagtt tccatttttg gcacaataga cttagtcaat
     8521 ggcacaagta ccacatcctt ctcagggaca aagtcatcca caacaacgac ctgtcccggt
     8581 agccccagcc aattctgcgg ttttggcttg caagtcacaa caccgggctc gatcgatcta
     8641 cagggagtga tagggaacga taacttcctg tggggcgcca atttgaatgc cggccaggat
     8701 gtaaacattg tggatagcat cttcaatgcc aactccacag aagtaccaac atttatcgat
     8761 gatacgggca tattcattac agccggtgga aacgtttccc tcaacaacgt gactgccaac
     8821 gataaccgac tcttcggtgc gagcatcgat gctgttggaa atgtttccgt caataacagc
     8881 aatttcctga gcaacaatgg cgtgaccgtt tcaggcagtg gcgccactca gtatgatggt
     8941 attggtctgc aggtcaattc tgacagcggc atattcatca acaacacgaa cgcatctgga
     9001 aattcactct ttggtgccga cctgaccgct ggtggcgatg tagccattgc gaacagcatc
     9061 tttagtaaca ccacaacagg tgataccgca gctcctttag gcatgggctt acaaatcgtc
     9121 agtggccaga acgtattcat cgacacggtt actctggacg gtaatcaagt ggatggagca
     9181 agcatacagg ctaccggcga tgttttcctg gacttcatta cagccacgaa taacggggca
     9241 aacggcgtcg aagtggatgc cgcatgcacc aacctaaata gcggcacgta tagtggcaac
     9301 ggagagtatg gtttaagtct cgtcaacccc accctgaatc agttagttcc cccagtattt
     9361 tccggtaacg gcttgggtga tataaaccca gcggctcccg gtgcgtgccc gcctcccgta
     9421 tttccagtgg ttgacggcgg aaccacgaac ggtggagcaa ccggcggcgt tagcaccttg
     9481 tctggtagca tctctaaccg gaacgcctcg tttgttagca tgagcaattc cagctataaa
     9541 aacctggggt tgatcaatag tggtgcgctc acgcttaata atttgttcgc cggtaactat
     9601 caactgcttg gtgttagcag cacatcaaac ggaaacgtca ctgccctcgg cattttcacc
     9661 ggcaaatatg cctatgtgca ttcagcgcaa ggacttcaga ttgttttcct gacacagcca
     9721 gctattttca gcggcttgtg gtccaatgag tcctgagtaa aaaaactttt atattctctg
     9781 ctacaattga gaaacggcta tatgccgttt ctcaattttt aaggacggaa ccgcttatga
     9841 aaaaatttat gctgcttctg cttccatcca tcctgcttgt gggcatgctg atcagtggat
     9901 gtggaaatgt gcagaccgcc ataccaacta acacacaaga ggcgttgccc tctccctcta
     9961 catcaactcc caccatcaca tttactcctg acccatgtgg aaccgaaaac atccaggcag
    10021 aagttgacaa agttcatagg catatgcgcg aatttgatga tgcatccatt ttggcatcca
    10081 gcacgccgcg tgatcaatta agcactgcga ttgccgattt acaacgaatc cgccgagcag
    10141 cggaagatga agtgacagca tcgtgcctgg gcgatctaaa aagatatcag atccagcata
    10201 tgaatttagt gattgatacc ttaattgctt ttctgagtgg tactgaacaa gctgttcttg
    10261 atcaggggat tgccgcggcc cgtcagcagc acgatcaata tacgcttgaa ctcgcacgta
    10321 ttcttgggat taccgtagtc cctgcgacgc cagccgtcgt tccatcacca accccaaccc
    10381 cttagtaatt tctccgaatc caaaacgaaa aaaccacgcc ttgtgagcgt ggtttttttt
    10441 gcgtcggtcg agtttattcc ttgggaatca tgatccacat aagcaggtac acgagcacgc
    10501 cgggaacacc gcctgggata aatgcgatga tgaaccccag tctgaaccag aaggcactaa
    10561 ttccgaaaaa ttcagccagc ccgccacaga ctccggcaat catactattg ctgcgcgaac
    10621 gacgcaggga cctcttttgt tgtgttgtca ttttgcactc ctatcttttt atgtcaattc
    10681 tttatacgca ggtaatccag aaaagtagca aaattaggca tttcgaacat attggtcgaa
    10741 ccaatccagc gttttctgcc aggcatcctg agcagcttcg gcgttgaaac gtgtgcctgt
    10801 atcgttgtga aatgcgtgat cggcattggg ataaatcatt ttcgcataaa tcttcccatt
    10861 tgcagccatg gcttcttcga tctgcggaat gctgccattg atacggctat ccaattctcc
    10921 gtagatcgcc agaacggcag cgttaatatt cggaacgtct tccaccggcg gaggaggacc
    10981 ataaaaagga atggccgcca ataactctga catttgggtg gccacccgcc aggtaacacc
    11041 tcctccaaag caaaatccgg tcatgccgac cctcgccgta tctgcaaagg ataaatttgt
    11101 cagatactgc cagccactct taaaatcttc aacgaattga tccggtaaaa tgttacccag
    11161 cgcaccgggc acatttcctt cgccaacaac agtgctgcct ccctggcgcg acaagaggtc
    11221 caccgccagc ccaacatacc cggctttcgc gaatcgtcgt gtcacatcct caatgtgggg
    11281 agtgagtccg cggttttcgt ggca
//
LOCUS       DASTJP010000425         5597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_363847, whole genome shotgun sequence.
ACCESSION   DASTJP010000425 DASTJP010000000
VERSION     DASTJP010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363847"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFQ23_12325"
     CDS             complement(<1..229)
                     /locus_tag="VFQ23_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907429.1"
                     /translation="MNGLTLSKDTKLEPRVYLLPEGISIDSDNVTLDGQGATIIGVDK
                     TGEGIKIFGRKNVTIKNLRVMNYYHGISIKKS"
     gene            537..1790
                     /locus_tag="VFQ23_12330"
     CDS             537..1790
                     /locus_tag="VFQ23_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9907430.1"
                     /translation="MTNFLIRNGTLIDGTGTTPLADAAVLVKENRIDSVGRFGSIRLP
                     DAQVTEIDAGGGYILPGLIDTHVHVMLEGVNIARDIVTPFSMRFFNAVNYLKNTINAG
                     ITSVRDAGGADAGVKQAVESGVIAGPRMQISISVLTITGGHGDGWMRSGIDFELFPAY
                     PGFPACRVDGVEDCRRKVREVLRAGAEIIKICSTGGVLSPTDHPEFTQFSPEELDVIV
                     REAAYRRGVKVMAHAQGAEGIKNAIRAGVHSIEHGIYLDEEIIDLMLKHGTFLVPTLL
                     APLAVLEAGEKGGMPEYGVRKSREVVEIHSESISRAHKAGVKIAMGTDAGVMPHGTNL
                     RELGLMVNIGMKPMESIVATTKIAAECLGWQDQVGTIEPGKLADIIITKTNPLQDIRS
                     LENVDNIPFVMKDGKVFKDLRSKAN"
     gene            complement(1811..5152)
                     /locus_tag="VFQ23_12335"
     CDS             complement(1811..5152)
                     /locus_tag="VFQ23_12335"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9907431.1"
                     /translation="MYRPKLASLVVAVALLITQTVPRAMADSCDSAGFVSDLTVPDGA
                     VFAPGASFTKTWRLMNSGTCAWTTSYMITFAGGDLMSAPQSVRLPVNIPAGQMVDISI
                     GLKAPTSSGHYKGLWKLSNASGIQFGIGASGNDAFWVDINVVDVSAVIFDFVANAPYA
                     QWKSGAGVLPFPGTSGDSRGYSFQINTPHLEDDSIDSSPALLTVPQNKLNGYIQATYP
                     EFQIQSGDKLQTLVNCEFGATGCYVTFRIDYLTSTGVQKTLWSWREAYDRRFYRANLD
                     LSSLAGQKVRFIFMLLSSGVASGDRAIWGSPRIVRTGTVQPPAPPSTLTPLPALTPTS
                     TPFIQPPPVISPSGCDKASFVTDVTVPDGTLFAPGAAFTKTWRLKNAGSCTWTTAYKF
                     LFYSGEQMSAPTSINIPSTVRPSGTVDLTVNMVAPGSPGKFRGNWVLSNANGALFGIS
                     TSATMPIWVEINVAGDSPIGSGYDFTANACSAVWKSGAGILPCPGTDNDAKGFVLKQD
                     APKLEDGSTGTPGLLTFPQNRYNGQIQGLYPTFTVQPGDRFQSVVGCEFGISCYVTFR
                     LDYMNANGVVKTFWSWREQNDRRPYAANIDLSPLVGQSVRFILTILATGYSTNDRALW
                     VSPRISRAGGTSPTVTPTSPTNDWLTHTNSTYGFQFRYPMGSVVAAGGNDNFTRIDLP
                     FTQGTNLADKYLEVIVNENANPCQSPLPTNHPSETVLINGISFLKQTGDEGAAGNRYQ
                     WVAYSTLRNNACISMNFILHSLAAGAFTPPVPEFDYAAETTVIPQMMGTFALLAPTPT
                     PTQTLTAVPPTATATSPIGDWLAYTNSYYGFQLRYPPRLEDLPGSNPNYVRIDLSFAQ
                     GTNLREKYLEVFVQENANPCQSPLPSPFSETVTINGLTFLKQTGEVGGAGQLHQWIAY
                     STQRDNICVSLDFILHSLNPGNLPTPPPVFDFATEAAVFEQMVATFTWLPSTPVPTAT
                     ATLIPPVTVPPPVTMTALVDALNAQNYDRLKSMMNQTFIFAFWQSQGYATTPEVAIET
                     LKTTYLGPTTHLSPDPGKDLVALLGGLDPYAVMGLNSSNSEIVFVSGWGPSGTDEAIL
                     YLNRWPDGTVFWHGVLIAPGGFVHP"
BASE COUNT         1386 a         1385 c         1490 g         1336 t
ORIGIN      
        1 ctgatttttt gatcgatatt ccatggtaat aattcatcac gcgcaggttt ttgatcgtta
       61 cgttttttcg accgaatatt tttatccctt cgccagtttt atcgactcca attatggttg
      121 caccctgtcc atccagggtc acattatcgg aatcaatcga aatcccttcc ggcaataaat
      181 agaccctggg ttccagtttt gtatctttac taagcgtaag cccattcata aagttaacct
      241 gtgactgtag atttgctgat agtatagcgc caatcattcc aaaatcaatg aactgtttac
      301 atggggagtg tcaaaaagga tgtgctaata gccgttttgc cacagagctc gcggagtctt
      361 ttgagttttt ttctcaagga actcagtgat ctctgtggct aagcccacga aattagacca
      421 tccctttaca tagcgaaacc tcaccttcaa cctctctccc agatggagtt tgtatttcac
      481 aggcgtgcta tactcccgac atcagattaa ctccacaatc caatagaggt atccacatga
      541 caaactttct gattcgcaac ggtacgttga tcgatggaac gggaacgacg ccactagcgg
      601 atgccgcggt gctcgtcaag gaaaatcgca ttgattcagt aggacggttc ggtagtattc
      661 gcctgccgga tgctcaggtc actgaaatcg acgcgggcgg aggctatatt ctgccgggac
      721 tcatcgacac gcatgttcat gtgatgctcg aaggtgtgaa tatcgcccgc gacattgtca
      781 caccgttctc gatgcgcttt ttcaatgctg tcaattactt gaagaatacg atcaacgccg
      841 gcattaccag cgtgcgcgat gcgggtggtg cggacgcggg cgtcaagcag gcagtggaaa
      901 gcggagtcat cgcgggtccg cgtatgcaaa tcagcatcag cgtgctcacc atcacaggcg
      961 gtcacggcga tggctggatg cgctccggga tcgacttcga attatttcca gcgtacccgg
     1021 gtttccccgc ttgccgcgtc gacggcgtag aagattgtcg tcgtaaagta cgtgaggtgt
     1081 tacgcgccgg agccgaaatc atcaaaatct gttcgacggg tggtgtactg agccccaccg
     1141 atcacccgga gttcacacag ttctcgcctg aagagttgga tgtgatcgtg cgcgaagcag
     1201 cctaccgaag aggtgtaaag gtgatggcac atgcacaggg cgcagaggga atcaaaaacg
     1261 cgatccgcgc cggagtccat tcaatcgaac atggcattta ccttgacgag gaaatcattg
     1321 atctaatgct caagcatggc acgttccttg ttccaacctt actcgcaccg ttggccgtac
     1381 tggaagccgg tgaaaagggt ggtatgcctg aatacggggt gcgcaagtcg cgcgaagtgg
     1441 ttgaaatcca cagcgaaagt atcagtcggg ctcacaaggc gggcgtcaag attgccatgg
     1501 gaaccgacgc gggcgtgatg ccgcacggca cgaatctgcg cgaactgggt ttgatggtca
     1561 atatcggaat gaagccaatg gaaagcattg tcgcaacaac aaagatcgcg gcggaatgcc
     1621 tgggctggca ggatcaggtg ggaaccatcg agcccgggaa actcgcggat atcatcatca
     1681 cgaagacgaa tcctttacag gatatccgtt cgctggaaaa tgttgacaat atcccattcg
     1741 tgatgaagga tggcaaagtt ttcaaagatc tgcgcagcaa agcgaattga gcgctggaag
     1801 ctttctctaa tcacggatgc acgaaaccac ccggcgcgat gagcactcca tgccagaaga
     1861 ctgtgccatc tggccaacga tttagataaa ggatagcttc gtctgtacca gacggtcccc
     1921 atccggagac aaacactatc tctgagttgc tggagttcag acccatgact gcatatgggt
     1981 cgagtccgcc taatagcgca accaagtcct tgccaggatc agggctgaga tgcgtagttg
     2041 gtccaaggta agttgtcttc aatgtctcaa tagccacttc aggcgtggtt gcgtatccct
     2101 gggattgcca gaaagcgaag atgaatgttt gattcatcat cgatttcaac cgatcataat
     2161 tttgcgcgtt caatgcgtcg accagggccg tcatagttac cggtggaggg accgtcacgg
     2221 gtggaatgag cgtcgcagtt gcagttggaa ctggtgttga aggcaaccag gtgaaggttg
     2281 caaccatctg ctcaaagaca gccgcctcgg ttgcaaagtc gaagaccggt ggtggagtgg
     2341 ggagattacc cgggttgagt gaatgcagga taaagtccag gctgacgcaa atattatctc
     2401 tctgcgtgga atacgcaatc cattgatgga gctgacctgc tccgcctacc tctcccgtct
     2461 gcttcaggaa agtgagtccg tttattgtta cagtctcgga gaatggagaa ggcagtgggc
     2521 tttgacaggg attcgcattt tcctggacaa agacttcgag gtatttctca cgcagattcg
     2581 ttccctgtgc gaatgataag tcgatgcgaa cgtagttggg attcgagccc ggcaggtctt
     2641 cgaggcgtgg tggatacctc agttggaaac cgtaatacga gttggtatat gctaaccagt
     2701 cgccaatggg agaggtcgct gtagcggtcg gaggaaccgc cgtgagtgtt tgggtaggcg
     2761 ttggcgtggg agccaaaagc gcaaacgtcc ccatcatttg agggatcacc gtcgtttcag
     2821 ccgcgtaatc aaactctggt actggaggcg tgaaagcacc tgcagccagc gagtgcagga
     2881 tgaaattcat gctgatgcag gcattgttgc gcagcgttga ataagcgacc cactgatatc
     2941 tgtttcctgc ggcaccttcg tccccggttt gtttgaggaa ggaaatgccg ttgatcagga
     3001 ctgtctcaga cgggtgattc gtcggaagtg ggctctgaca cgggttagcg ttctcgttga
     3061 cgatcacttc cagatatttg tccgccaggt tcgttccctg tgtaaagggc aggtcaatgc
     3121 gcgtgaagtt gtcgttccca cccgcggcga ctacactccc cattggatat ctgaactgga
     3181 agccatatgt agagttggtg tgagtcagcc aatcatttgt cggtgaggtc ggggtaaccg
     3241 tgggagacgt gccacctgcg cgcgagatgc gcggtgatac ccacagagcg cgatcatttg
     3301 tcgaatatcc tgttgcgaga atagtcagga tgaagcgtac gctttgcccc accagcggac
     3361 ttaaatcaat atttgcagcg taggggcgcc ggtcgttttg ttcacgccag gaccagaacg
     3421 ttttgaccac accgttggca ttcatataat caagccggaa ggtaacataa caactgatcc
     3481 caaattcgca gcctacaaca gactgaaaac gatcaccggg ttgaacagtg aaggtgggat
     3541 acagcccctg aatctgaccg ttatatctgt tttgggggaa ggtcaataaa ccgggtgtac
     3601 cggttgaccc atcttcaagt ttaggcgcgt cttgtttgag gacaaacccc tttgcgtcgt
     3661 tgtccgtgcc cgggcaggga agaatgcccg caccactctt ccaaaccgct gaacaggcat
     3721 tcgctgtgaa atcatagccc gagccgatcg gcgaatcgcc cgccacattg atctcaaccc
     3781 agatcggcat cgttgcactg gtactgattc cgaacaatgc gccattggcg ttgcttagta
     3841 cccaattgcc gcgaaatttt cccgggctgc cgggagcaac catgttgacc gtgagatcaa
     3901 ctgttccgct aggtcgtaca gttgatggaa tgttaataga ggtcggcgcg ctcatctgct
     3961 ccccactgta gaacaagaac ttgtatgcag tggtccatgt gcatgagcca gcgttcttca
     4021 aacgccatgt cttggtgaag gcagcgcccg gcgcgaacag ggtcccatca ggcacggtga
     4081 catctgtaac aaaggaggct ttatcacaac cggatggtga aataacaggc ggaggttgaa
     4141 tgaacggcgt ggatgttggc gtcaatgccg ggagcggggt aagagtcgac ggaggtgcgg
     4201 gaggttgaac agtcccggtt cgaacgatgc gcggcgatcc ccagatggcg cggtcaccac
     4261 ttgctactcc actggaaagc agcataaata taaagcggac tttttgcccg gcgagcgaac
     4321 tcaagtccag gttagcgcga taaaaacgtc tgtcgtatgc ctccctccag gaccagagtg
     4381 ttttttggac accagtggag gtcagataat caatgcggaa cgtgacgtag cagcccgttg
     4441 ccccaaattc acaattgacc agcgtttgta atttatctcc cgactgaatt tggaattccg
     4501 gataggtggc ttgaatatat ccatttaact tattctgcgg aaccgttaat aaagctggag
     4561 atgaatcaat cgaatcgtct tcgagatgcg gcgtgtttat ctgaaaggaa tacccccggc
     4621 tatcgccgct ggtacctggg aaaggcagca cacccgcgcc gcttttccat tgagcgtagg
     4681 gcgcgttcgc aacgaagtcg aagatcactg cactgacgtc aaccacgttg atatccaccc
     4741 agaacgcgtc gttgccagac gcaccaatgc caaattgaat gccagacgca ttacttagct
     4801 tccataagcc tttatagtgt ccgcttgatg ttggtgcctt caatccgatc gagatatcca
     4861 ccatttgccc tgccggtata tttactggaa gccttacaga ttgtggcgcg ctcattaggt
     4921 cgccaccggc aaaagtaatc atgtaggacg ttgtccatgc acacgtaccg ctgttcatca
     4981 aacgccaggt cttggtaaag gatgcccccg gcgcaaagac cgctccatcg ggaacggtta
     5041 aatcagaaac aaagcctgct gaatcacagg agtccgccat agcacgcgga acagtttgtg
     5101 taatcaacaa tgctacggca acgacaagac tggcgagctt tggtctatac atgtcatctc
     5161 ctgctgtttg cccactgaaa acggcttctt cctgattaat gcatcggtag gtgaaaaacc
     5221 acctctatcc tcgcgccatt cttcctatta aaagatgaca acttcgattc aatatttaga
     5281 gtctcctgcg cgcgtggtag catcgtttgg gccatcgtgc gggttgcact agctgggtta
     5341 acgaattaac agaattgacg attgtgttcc tgtgaaaagt aagtaagata ggaaacgatt
     5401 ttcctggttt caagcaatac agagggaaaa ttaatatata acaaggagga attaacgatg
     5461 aaacgttttc cgaattttgt cttattggtt ctactatttc tcacagcctg tggaggtagt
     5521 ccagccttaa atcctacggc cactcctgct ccaactcaga ctctcgaacc aacccaaacc
     5581 ccacaaccca ccgagac
//
LOCUS       DASTJP010000426         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_9229,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000426 DASTJP010000000
VERSION     DASTJP010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9229"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFQ23_12340"
     CDS             <1..671
                     /locus_tag="VFQ23_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9907432.1"
                     /translation="GIAFDNYIEAFSKLAPSLGNSFLMVIPSALLSSALGSLNGYILA
                     KWKFRGADVIFPLILFGMFIPYQSILIPLVQFMNAIGVTGLPGLALAHIIYGIPITTL
                     TFRNYYAGLPDELLEAAKIDGASMLGIYRHILLPLSIPSFVVVLIWQFTSAWNDFLFA
                     VVLTGNQDWPITVALNNMAGSQIIAWNVQMAGSLLAALPTLLVYIFLGRYFLRGLLAG
                     SLKG"
     gene            complement(726..>2205)
                     /locus_tag="VFQ23_12345"
     CDS             complement(726..>2205)
                     /locus_tag="VFQ23_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907433.1"
                     /translation="KMMLTDFPAAIGMALVVLLVCEWLKNPQQKWYYALWIGGVIGLA
                     IMLRTNTLLILLLIPLFSIFSFAQNWRMWFASSFLLILAVFAITLPWELRNQSLGGQM
                     YSSFVTKFQSVIDLRYQSPLLPESSVPQPDVLSSVLLRQTTVLVDIYKDIELIREPSC
                     NTVVCFVSNHFFHNILTSVLVLPTSPVLDDLRHTVKESYPYWRPDWDGSFTIGSLLFF
                     VLNIFFIGLGITVAWKQHRLTGLVPLAIFVFYNLSNGFARTSGGRYIVPIDWIVTIYF
                     LIGVFHVILSLASSLGFRWTLFAESQEQDPPKQSSMNRSDLLRGLGILAILLFAGSFI
                     PIAEMLHTDRYENFDVAKALSEHESQIASAGLSLLEIDSFLKNPNAEISVGRALYPRY
                     YIEFEGEVHFYPVVVMGFPRTTFTLIGPDGEKGIVLPGEKPDSFPHAVDTIVLGCKEE
                     FYVDALAVIVLDETGSVYTRSPASPLQCPLKQPVCDNNHNCN"
BASE COUNT          570 a          515 c          560 g          560 t
ORIGIN      
        1 gcggcatcgc gtttgataat tacatcgaag cgttttccaa actggcgcca tctctgggaa
       61 atagtttttt gatggtgatt ccctcggctc ttctctcctc cgcgctgggc tcactgaacg
      121 gatatatcct tgccaaatgg aaattccgcg gcgcggatgt gatcttccct ctgatcttgt
      181 tcgggatgtt catcccctat caaagtatcc tgattcccct ggtgcagttc atgaatgcaa
      241 ttggtgttac cggcttgcca gggctggcgc tggcgcatat catttatggc attccgatca
      301 cgacccttac tttccgcaat tattatgccg gtttgcccga tgagttgctt gaagctgcga
      361 agatcgatgg cgcgagtatg cttggcattt atcgccatat tttgctgccc ctgtccattc
      421 caagctttgt tgtggtcctg atctggcaat ttacttctgc ctggaatgat tttctcttcg
      481 cggttgttct cacagggaat caagattggc cgattacagt ggcgctcaat aacatggcgg
      541 gcagccagat cattgcgtgg aatgtgcaga tggcgggttc tctcctcgca gcactgccga
      601 ccctgctggt ctatattttc ctcggtcgct acttcctgcg gggcttgctg gctggttcgc
      661 ttaagggata aataatcgct tcaaaatgga gcaggcgata tgcggcagcc ctggcagggg
      721 gctctttagt tgcaattatg gttgttgtcg cagactggtt gtttaagggg gcattgcaga
      781 ggggatgcgg ggctgcgggt ataaacggat cctgtttcgt caaggacgat cacggccagg
      841 gcatccacat agaattcttc tttgcagccg agaacgatcg tatcgaccgc gtggggaaaa
      901 gaatcaggct tttcacccgg caggacgatc cccttctctc catcaggacc gatcagggtg
      961 aacgtggtac gtggaaatcc cattacgacg acggggtaga agtggacctc gccttcgaac
     1021 tcgatgtagt accggggata taaggcacgt ccaactgaaa tttcagcatt tggatttttt
     1081 agaaacgaat cgatttccag cagactcaac ccggcgcttg cgatctgtga ctcatgctcg
     1141 gaaagcgcct ttgcgacatc aaaattttcg tagcggtctg tatgcagcat ttccgcgatg
     1201 ggaatgaatg atcccgcgaa gagcaggata gcaaggatac ccagacctct cagcaaatcg
     1261 cttctattca tcgagctttg cttcggagga tcctgttctt gggattccgc gaaaagagtc
     1321 catctgaagc ccaaagaact tgcaagtgaa agaattacgt gaaaaacacc gatcaggaag
     1381 taaatcgtaa cgatccaatc tatggggaca atgtatcttc cacctgatgt gcgtgcaaaa
     1441 ccgttggaca gattatagaa aacaaaaatc gccagcggca cgagccctgt caagcggtgt
     1501 tgtttccagg caactgtgat tcccaaccct ataaaaaata tgtttaacac aaagaagagg
     1561 agggagccaa ttgtaaacga tccgtcccag tctggtcgcc agtacggata gctttctttt
     1621 accgtgtggc gtaaatcgtc gaggaccggg gatgtgggca aaactaaaac ggatgtcagt
     1681 atattatgga aaaaatggtt cgacacaaag caaacgacag tgttacagga aggttctcga
     1741 attaattcga tatccttata aatgtccacc aggaccgttg tttgtcttag caaaacagag
     1801 gacaaaacgt ccggctgagg aacagaactc tcgggtagta ggggcgattg atatcgcagg
     1861 tcgataacac tctggaactt ggtaacaaag gagctgtaca tttgcccgcc cagagattga
     1921 tttctcaact cccagggtaa tgtaatagca aatacggcaa ggataagaag aaatgaactg
     1981 gcgaaccaca ttctccaatt ttgtgcaaat gaaaaaatgg agaaaagggg tatgagcaac
     2041 aaaatcaaaa gcgtatttgt cctcaacatg attgccagtc caatgactcc gccgatccat
     2101 aaagcgtaat accacttttg ctgagggttc ttgagccact cgcagaccaa caagacaacc
     2161 agcgccatgc cgattgcggc tggaaaatca gtgagcatca ttttg
//
LOCUS       DASTJP010000427         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_9791,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000427 DASTJP010000000
VERSION     DASTJP010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9791"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..2196)
                     /locus_tag="VFQ23_12350"
     CDS             complement(163..2196)
                     /locus_tag="VFQ23_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907434.1"
                     /translation="MTQTIQRLFKNESFIAALLILLTTVITYGVSIPNLGYYHDDWFV
                     LWSGQARGADSLIPLFSTDRPFMGVVYSVVYRLLGDSIVNWHLYALLWRFIGGMAFFW
                     ILRLIWPEQKYLTTVMTVLFLVYPGFLSQPNANTKQNHLYGFGTALLSIALMLQAMKT
                     DKQGWKIVYSVLSLILTANYLWIYEYMIGFEGTRLLLLGYAMFQKGFRQFRSLAIETL
                     KRIWPYWLVTAAFLYWRVFIFQGERNATDVSGLAGSYLSNFRYMSIRLILETVKDFLD
                     TTVFAWFVETYHLFSIAPYSNLVFALLIAAVVAALLWLYTSLFKKQWGNDVNEHETPR
                     LMKDLLWMGALIILFAISPVIFSDRHIDLYDPYKSYGLHPIPGVVLFILGLLLMLQPR
                     FRNWALVALVGISVSAQILNADYWERYWDIQRDMAWQLTWRAPDIQDDTLVMASISGG
                     FNPQQDYEIWGPINLIYNPGPAKAPAIQAEVLNADTAYSILRQEVKNNHVRDIRLRRD
                     FTSLLLITYPSSVSCFHVIDGNLPAYSGSESLLVRQVGAYSQADRILSAGTSPVPPAS
                     IFGSEPERGWCYYYQKAALARQSGDWEEIGRLYDQVRALGLETDDKSEIIPFFEGLVN
                     LGRYEDAKDLYREQIKGQTEVRFPLCSSLANDPGYAPAFRYDYQKINELLCKS"
BASE COUNT          628 a          627 c          626 g          561 t
ORIGIN      
        1 tggcgcaaac gcgtcatttt atcgagatcg ttcgcgggga gtctgaaccg gtctgcactc
       61 tcgaagatgg gatgtcggct ttacgcctcg cgctggcggc gtatgaatct cagaaaaccg
      121 gaagcctggt caaaatcgcg aaagcggtct gagaattcgc tttcacgatt tgcacagcag
      181 ctcattgatt ttttgatagt cgtatctaaa ggctggtgcg tagccgggat cgttggctaa
      241 agaactgcac aacgggaagc gtacttcggt ctgccctttg atctgttctc tgtaaaggtc
      301 tttggcatct tcatatcgcc ctagattcac aagcccttca aagaacggga ttatttccga
      361 cttgtcatct gtttcgagac ccagagcgcg cacctgatca tataatctgc cgatctcttc
      421 ccagtcgccg ctttgccggg caagggcagc tttttgatag taatagcacc agccacgttc
      481 aggctcgcta ccgaaaatgg atgccggtgg aacgggcgac gtcccagcgg agaggatccg
      541 gtccgcctgc gaatatgcgc cgacctgccg cacgagaagc gattcgctcc ctgagtaggc
      601 aggcaagttt ccatcaatca cgtggaaaca ggacacggaa gagggataag tgatcaagag
      661 cagactggtg aaatccctgc gaagccgaat atcacgaacg tggttgttct tcacttcttg
      721 ccgcaagatg ctataggctg tatccgcgtt gagcacttct gcctggatgg cgggggcttt
      781 cgcgggtcca ggattgtaga tgagattgat cggtccccag atctcgtaat cttgttgggg
      841 gttgaagcct cccgagatgg atgccatcac aagcgtgtca tcctggatat ccggtgcgcg
      901 ccaggtgagc tgccatgcca tatcgcgttg aatatcccag tagcgttccc aataatcggc
      961 gttcaagatc tgggcggaga ccgagatgcc gaccaacgcg accagtgccc aattgcgaaa
     1021 ccttggctgg agcataagca acaacccaag aatgaatagc accacgcccg ggatgggatg
     1081 aagcccgtaa ctcttgtagg gatcataaag gtctatgtga cggtccgaaa atatcacggg
     1141 cgagattgcg aacaagatga tcagcgcgcc catccagagg agatctttca tcaagcgcgg
     1201 tgtctcatgt tcattcacat cgtttcccca ttgtttcttg aacagcgatg tgtaaagcca
     1261 aagtaaggcc gctacaactg cagcaatgag tagggcaaac accaaattgg agtagggcgc
     1321 gatcgaaaac aggtgatagg tttccacgaa ccaagcaaaa acagtcgtgt cgagaaaatc
     1381 cttcacggtt tccaggatca gccggatgga catgtaacga aaattactga gatagcttcc
     1441 cgctagccct gagacatctg tcgcattgcg ctccccctgg aagataaaca cgcgccagta
     1501 aagaaatgcc gctgtcacga gccaataggg ccagatcctc ttcagggtct ctattgctaa
     1561 agagcgaaac tgcctaaacc ctttttgaaa catagcatag ccaagcagca gaagacgcgt
     1621 cccctcaaag ccgatcatgt actcgtagat ccataagtaa ttcgcggtca ggatcaagga
     1681 gagaacgctg tacacgattt tccacccctg cttatcagtc ttcatcgctt gcaacattaa
     1741 ggcaatcgag agcagggctg ttccgaaccc gtaaagatgg ttctgttttg tgtttgcgtt
     1801 tggctgggaa agaaaccccg ggtagacgag aaaaagcaca gtcattaccg ttgtgagata
     1861 cttttgctcc ggccagatca gacgcaggat ccagaagaac gccataccgc cgataaaacg
     1921 ccagagcagc gcgtacagat gccagttgac gatcgaatcg cccagcagac gatagacgac
     1981 cgaatagacc acgcccatga aaggccgatc tgtggaaaac aatgggatga ggctatctgc
     2041 cccgcgtgcc tgaccagacc agagcacaaa ccagtcatcg tgatagtaac cgaggttggg
     2101 aatgctcacc ccgtaggtaa tgacagttgt cagcaagatc agcagagcag caatgaagct
     2161 ctcatttttg aacagtcttt gtattgtttg cgtcatccgt ggttcctggc ttcgccaaaa
     2221 ttgtaacatg acgcggctta caggctgttc agacaccctg ctcaattcat acaattcctg
     2281 gcaatttcgc gatgatataa tgacctcccg aactgcgtca tgaaaactca atcgctgccg
     2341 agaaccaagt ggaattcatt gggggatctt cttttgcctg tcctcgttgc tctgatcttc
     2401 ctcgctttca cattttcgta ttacccgttt cgcgagaaac tt
//
LOCUS       DASTJP010000428         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1117349, whole genome shotgun sequence.
ACCESSION   DASTJP010000428 DASTJP010000000
VERSION     DASTJP010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117349"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFQ23_12355"
     CDS             complement(<1..339)
                     /locus_tag="VFQ23_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HET9907435.1"
                     /translation="MTELWTLTEDTFDPKKQYHKETIFTIGNGYLCTRGAFEEGFPND
                     HRATFIHGVFDAAPIVFTELANAPDWLPLTVFLNDERFSLDSGTIETFQRNLDLQTGV
                     LTRTVRWRSPS"
     gene            complement(345..1265)
                     /locus_tag="VFQ23_12360"
     CDS             complement(345..1265)
                     /locus_tag="VFQ23_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HET9907436.1"
                     /translation="MKLQRHPANPLLLPDPTSDWETYNVFNPSVIYHNNLFHMHYRAQ
                     GMDWISRVGYAVSADGIHWNRLRRPVVAPQDSSDSRGIEDPRVTEIDGRFYMCYTAYS
                     SEVAEGVKSTHAGGGVTPTIAVSDNLIRWERLGSIVRGEDNKDHVLFPRRINGRYVAL
                     HRRPPQVWLAESDDLLHWPEEHMRPIFGPRPGNGWDEKRVGGNGVPIETEHGWLMLYH
                     AYNNDHVYRLGACLLDLEDPSRVINRPKDLIFEPKELWELRGDVPNVVFSCANPVVND
                     TVYVYYGGADHVIALATCTLDDLLNFARHG"
     gene            complement(1272..2072)
                     /locus_tag="VFQ23_12365"
     CDS             complement(1272..2072)
                     /locus_tag="VFQ23_12365"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9907437.1"
                     /translation="MEALRLYWRLVGLSMQARLQYRADFIMGILNVIALNAVNLGLIG
                     IIVYRFVDLNGWVVWELFFLYCLWLLGHSLYSLFFWHFWEMEEYLIQGTFDQFLLRPA
                     SPFVQFLGREVQYIGFADMSVAIAGLVLAYINLELQWSLVQWLYFPIIVISGTVIETA
                     LTLMTACIAFWTGRSDMAINTIMEFNVLVQHYPIDIFGVWFRVIVTGIIPVAFMNYYP
                     ALMLLGKLEANSSMAWLGYISPLVALILTALASGVWHVALRRYSSSGG"
     gene            complement(2076..>2564)
                     /locus_tag="VFQ23_12370"
     CDS             complement(2076..>2564)
                     /locus_tag="VFQ23_12370"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9907438.1"
                     /translation="HMLARSTGEMMIGVSVLAIPALIIGFLFLGLQFPPDLPTALLSI
                     VSVLLGFLLFFHLNFILGSLAIVALDIRHISWAYFSIVRFLGGQVVPLWLFPPVVAAI
                     AEVLPFKGTYYIPISIYVGRLTGVDAIRGLEFQIVWLIALAVVSRMLWGWAHRRLVVQ
                     GG"
BASE COUNT          710 a          665 c          662 g          527 t
ORIGIN      
        1 tgagggtgaa cgccagcgga cagtacgagt tagtacccct gtttgaagat cgagattcct
       61 ttgaaacgtc tcaatggtac ctgagtccag agagaaccgc tcgtcgttga ggaaaacagt
      121 tagtggaagc caatcaggcg cgttggcgag ttcggtaaag acaatcggcg cggcatcgaa
      181 cacgccatga ataaatgtag cacggtggtc atttggaaag ccttcttcaa atgcgccgcg
      241 cgtacaaaga tagccgtttc caatcgtaaa tatggtttct ttgtgatatt gctttttagg
      301 gtcgaacgtg tcttcagtaa gcgtccagag ctcagtcatt atccttatcc atggcgagca
      361 aaattcagca agtcatccag cgtgcaggta gcgagtgcaa tcacgtgatc tgcgccgccg
      421 taatatacat acacggtgtc atttacgaca gggttcgcac aggaaaacac cacgtttggc
      481 acatcgcctc gtaactccca caattccttt ggttcgaaga tcagatcttt tggacgattg
      541 atcacgcgtg acggatcctc gagatcaaga agacaggctc ccagcctgta cacgtgatca
      601 ttgttgtagg cgtgataaag catcagccag ccgtgttctg tttcgattgg cacgccgttg
      661 ccgccaacgc gtttttcatc ccagccattg ccgggccgcg gaccgaaaat ggggcgcatg
      721 tgctcttcgg gccaatggag caagtcgtca ctttctgcga gccacacttg aggcggccgc
      781 cgatgcaaag ccacataacg tccattgatg cgtcgaggaa acagcacatg gtctttgttg
      841 tcttcgccac gcacaatcga gcccaggcgt tcccaacgaa taagattatc gctgaccgcg
      901 atggtgggag tcacgccccc gcccgcgtga gttgacttca caccctcagc gacttcgctg
      961 ctataggcgg tatagcacat atagaatcgc ccgtcgattt cagtcacacg cggatcttcg
     1021 attccgcgcg agtctgagga atcctgtggc gcaacgactg ggcgtcgcaa tcgattccag
     1081 tgaatgccat ctgcgctcac tgcgtatccg acacggctga tccagtccat gccctgggcg
     1141 cgatagtgca tgtgaaacag attgttatga tagatcacgg aagggttgaa gacgttgtag
     1201 gtctcccaat ctgaggttgg gtccgggaga agtagaggat tggcgggatg gcgttgaagt
     1261 ttcattggaa gttatcctcc ggagctggag tagcgtcgaa gcgccacatg ccaaacccca
     1321 gatgcaagcg cggtcagaat caaagcgacg agaggtgaaa tatagccgag ccatgccata
     1381 gagctatttg cctccagttt tcccagcagc atgagggcgg gatagtaatt cataaaagct
     1441 accggaatga ttcccgttac gatcacgcgg aaccaaacac caaagatatc aatcgggtaa
     1501 tgttgtacaa gtacattgaa ttccatgatg gtattgatgg ccatatctga acgccccgtc
     1561 caaaaggcaa tacaagccgt catcaacgtc agggcggttt cgattacggt acctgagatg
     1621 acgattattg gaaagtacag ccattgaacg agactccact gcaattcaag attgatatat
     1681 gccaggacaa gcccggcgat ggccaccgac atatccgcga aaccgatata ttgaacttca
     1741 cgtccgagaa actgaacaaa tggacttgct ggccgcagca ggaactgatc gaacgttccc
     1801 tggatcaaat attcctccat ttcccagaaa tgccagaaga acaggctgta gagactgtgc
     1861 ccgagcaacc agagacaata caggaagaag agttcccaaa ccacccagcc attcaaatca
     1921 acaaagcgat agacaataat accgatcagc cccagattga ccgcgttcaa cgcgatcacg
     1981 ttgagaatgc ccatgatgaa gtcggcgcga tactgcaggc gcgcctgcat ggaaagtccg
     2041 acgagtctcc agtaaagacg aagagcttcc atgaatcagc ctccctgtac cacgagccgt
     2101 cggtgagccc atccccagag catgcgactg acaaccgcca gcgcgatgag ccacacgatt
     2161 tgaaattcca acccacggat tgcatccact ccggtaagcc ggccgacata aatggagatt
     2221 ggaatgtaat aggtcccctt gaaaggcagt acctccgcga tcgccgcaac aactggcgga
     2281 aataaccaca atggcacaac ttgcccgccg aggaagcgaa caatgctgaa ataagcccaa
     2341 ctgatgtgcc gaatatcgag ggcaacgatc gccagagagc caaggataaa attaagatga
     2401 aaaaacagca gaaaccctaa cagaacactg acgatggata ataacgcggt aggcagatcg
     2461 ggtgggaact gtaaaccgag aaacaggaag ccgatgatca aggcagggat agccaatacg
     2521 ctgacgccaa tcatcatctc accggtgctg cgtgccagca tgtg
//
LOCUS       DASTJP010000429         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_765327, whole genome shotgun sequence.
ACCESSION   DASTJP010000429 DASTJP010000000
VERSION     DASTJP010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765327"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFQ23_12375"
     CDS             complement(<1..791)
                     /locus_tag="VFQ23_12375"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907439.1"
                     /translation="MPQEYIELRGARENNLKNVSLRIPKRKITIFTGVSGSGKSSIVF
                     DTIATESQRLLNENFSMFVRNFLPKYSQPDADAIENLSMSIVVDQKRMGGGSHSTVGT
                     ITDINTILRMMFSRIGQPHVGPTNVFGFNDPQGMCPNCNGLGRKLDVDLDKFLDKSKS
                     LNEGAILFPEYVVDSWYWSNLINTGLFDPDKKISKYTQKELNDLLYSEPIKVKTKVGA
                     KDFNMTYEGIVNRFTNKYIKRDLKTMSERTQKSVEPYMTMGPCSE"
     gene            complement(826..1302)
                     /locus_tag="VFQ23_12380"
     CDS             complement(826..1302)
                     /locus_tag="VFQ23_12380"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HET9907440.1"
                     /translation="MSFQAYLDNIEEKTGKTPKQFIAEAKKKKITAFKDIIAWLKTDY
                     GLGLGHARALAYVIQHGPNFEVKQTTGTHRDASGTLKLDGKANKKQAASLPDKLGAPA
                     ERALANAGIKNLKQLTKFSEAEIKQLHGVGPNAIGKLGQALAEAGLSFAGKKKSKK"
     gene            complement(1331..1906)
                     /locus_tag="VFQ23_12385"
     CDS             complement(1331..1906)
                     /locus_tag="VFQ23_12385"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9907441.1"
                     /translation="MGKVVLGMTMSLDGFINDRNGSVDALYPDLAAWRETESGKESIQ
                     NTGAVVMGRNSYAMSEDPDWFAGNYEYQVPIFVLTHAPPEKHPKETEQLTFTFVTDGI
                     QSAIQQAKVAAGDKEVNIIGAASTARQCLKERLADELHVDVMPIFLGSGLRVFEDLGT
                     ETIRLERIKVMEFPGGRTHLRFRIIKVDEGT"
     gene            complement(1918..2715)
                     /locus_tag="VFQ23_12390"
     CDS             complement(1918..2715)
                     /locus_tag="VFQ23_12390"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9907442.1"
                     /translation="MNENKSVDAGTVWWFWFTTNAFAVDKRLRRIMRILPHDPRCKFC
                     NTPFQGIGAMIARVLFGKQRSALNPRYCNLCEIASREFPGGAEVPMSMLFIDIRGSTA
                     LSEKMTPTEFSQIISRFYAAATKVIIEEDGLVEKLAGDEVAAFWGAGFAGPDYVHRTI
                     DVAQKLLQIMERQKIPVGIGVHSGVAYFGSLATAEGLTDISAKGEEVNMAARLASKAG
                     VGEIIVSEPALQAAGIDNSRLEARSLELKGISGPVNIRVMNGSSSFQ"
BASE COUNT          663 a          794 c          689 g          790 t
ORIGIN      
        1 cattcggaac agggacccat cgtcatgtaa ggctctaccg acttctgagt ccgctcggac
       61 atggttttta aatcccgctt gatgtacttg ttggtgaagc ggttgacgat gccttcgtag
      121 gtcatgttga agtcttttgc ccctactttg gtcttgacct tgatgggttc gctatacaaa
      181 aggtcgttca gttccttctg ggtgtatttg gatattttct tatcagggtc gaacaaacca
      241 gtgttgatta aattactcca ataccaactg tccacgacgt attccgggaa tagaatagcg
      301 ccctcgttca gagatttgga tttgtccagg aacttatcca ggtccacatc cagtttgcga
      361 ccgagtccat tgcagttcgg gcacatcccc tgcgggtcgt tgaaaccgaa tacgttggtg
      421 ggtcccacgt ggggctgccc gatgcgcgag aacatcatgc gcaggatcgt gttgatatcg
      481 gtgatcgtcc caacggtcga gtgagacccg ccgcccatcc gtttttgatc caccacgatg
      541 gacatgctca ggttttcgat ggcgtccgcg tcaggctggg aatacttcgg caggaagttc
      601 cgcacgaaca tgctgaagtt ttcgttgagc agccgctgcg actcggtggc gatcgtatcg
      661 aacacaatcg atgatttgcc ggaaccggag acgcctgtaa aaatggtaat cttgcgtttt
      721 ggaatgcgca atgaaacgtt ctttaaattg ttttcgcgcg caccacgtag ttcgatatat
      781 tcctggggca ttaaattctc ctataaacgt ccgagaccgc gagccctact ttttcgactt
      841 ctttttcccg gcaaacgata aacctgcttc tgccagagcc tggccaagtt ttccaattgc
      901 attaggacca acaccatgga gttgcttaat ctcggcttcg ctaaatttgg tcagctgctt
      961 gagattcttg atacctgcgt tggctaatgc acgttcggca ggcgcaccta gcttgtctgg
     1021 aagactggct gcctgctttt tgttcgcctt accatcgagt tttaatgtac ctgaagcatc
     1081 acggtgcgtt cccgtggtct gcttcacttc aaaatttgga ccatgctgaa tgacatacgc
     1141 cagtgccctg gcatgcccta aacccaatcc atagtctgtc ttaagccagg cgatgatgtc
     1201 cttgaatgca gtaattttct tcttctttgc ctccgcaata aactgcttgg gggttttgcc
     1261 tgttttttct tcgatattgt ccaaatatgc ttgaaatgac atggtttctc caatctggtt
     1321 tgtaagagtt ttatgtgccc tcatctactt taatgatacg gaatcgcagg tgtgttctgc
     1381 cgcctggaaa ttccatcacc tttattcgct ccaatcgaat cgtctcggtg ccaagatcct
     1441 cgaatacacg caaaccgctg ccgaggaata tcggcatgac atccacgtgc aattcatcgg
     1501 cgagtctctc tttcaggcat tgccgcgccg tgcttgcggc accaatgata ttcacctcct
     1561 tatcccccgc agccactttc gcctgctgta tcgcactttg gataccatcg gttacgaagg
     1621 taaaggtcag ttgctccgtt tcctttggat gcttttctgg cggcgcatgc gtaagaacaa
     1681 agattggaac ttgatattca tagtttcctg caaaccagtc cggatcttca gacatggcat
     1741 aagaattgcg tcccatcacc actgctccgg tattttgtat agattccttc cctgactcgg
     1801 tctctcgcca ggcagccagg tcaggataca gcgcatccac gctgccattg cgatcattga
     1861 taaacccatc caatgacatg gtcataccca gcacaacttt acccataaag actccgttta
     1921 ttgaaatgac gatgagccgt tcatcacacg gatattcacc ggcccgctga ttccttttaa
     1981 ctccaggctg cgcgcctcca ggcgactgtt atcgatacct gctgcctgga gggctggctc
     2041 gctgacgatg atctcgccca cgcctgcctt cgaagccagt cgagccgcca tattgacttc
     2101 ctcccctttt gccgagatgt cggtcaatcc ctcggctgtt gccagggaac caaagtaagc
     2161 aacacccgaa tgcacgccaa tgccaaccgg gatcttctgg cgctccatga tctgcaatag
     2221 cttttgggca acatctatcg ttctatggac ataatccgga ccggcaaacc cggcgcccca
     2281 gaacgctgcc acttcatcgc ccgccagctt ttccaccaat ccatcttctt cgatgatgac
     2341 ctttgttgcg gcagcataaa acctactgat gatttgacta aattccgtgg gggtcatttt
     2401 ctctgagagc gccgtcgagc cacggatgtc gatgaaaagc atagacatcg gaacctcagc
     2461 accccctgga aattcgcgcg aagcgatctc gcacaagttg cagtagcgag gattcaaagc
     2521 agagcgttgt ttaccaaaca acactcgagc aatcattgcg ccaattccct gaaaaggagt
     2581 attgcagaat ttacagcgcg ggtcatgcgg aagtattcgc atgattcgac ggaggcgctt
     2641 gtccacggca aacgcgttcg tcgtgaacca aaaccaccag acggtgccag catctacaga
     2701 tttgttctca ttcatgactg aagcctatca tcatgcctac gattaaaatt tgttcgtctt
     2761 tcgatgcctg atcttcgatg acaagcatta tgacacaaag aatccgaacc tggcaatccg
     2821 ctaaataagt acaggcgtcc ggtgatcggg gtgaccggct ggatcgagta cccaatgttc
     2881 ctgggcgatg gcgcgataca ggcgatatgg agctggcgcc cgtacatctt ccggtg
//
LOCUS       DASTJP010000430         8745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_142520, whole genome shotgun sequence.
ACCESSION   DASTJP010000430 DASTJP010000000
VERSION     DASTJP010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142520"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFQ23_12395"
     CDS             <1..409
                     /locus_tag="VFQ23_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907443.1"
                     /translation="FLIPGDGSYGIQPVFVEDLADLAVEGVYSQSNYIIDAVGPDAYT
                     FKELIQRIGKTIGAERPIISVPPRLALLAAQFLSLFVGDVILTPEEVDGLMANLLVSK
                     EPGRGKTAFKDWLEANKDIVGKKYASEIKKHYE"
     gene            complement(850..1473)
                     /locus_tag="VFQ23_12400"
     CDS             complement(850..1473)
                     /locus_tag="VFQ23_12400"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HET9907444.1"
                     /translation="MKLPDQKSINFKVPDQLAERLPYRLRETFLSTPELALLNALQEM
                     AGRHYVICPKVALNDIFYIVRPNENVHFFNKIFRKHVDFLLCETPGFKPAIGVELVKP
                     VSRNETRDTDQFMEDLFVSAGLPLVHVPSNERYLQDDLVELFQLAITKVKKTGPLRAT
                     TKSDSVPMCPVCGKMMVLRIHRNGFGGGKRYYGCMDSPKCNGIVPID"
     gene            complement(1502..2272)
                     /locus_tag="VFQ23_12405"
     CDS             complement(1502..2272)
                     /locus_tag="VFQ23_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9907445.1"
                     /translation="MNLQSYLTFARQLAYRAGRITLSYYNKGIQHDMKQDDSPVTAAD
                     QATEEFIRGEIEKNYPTHALVGEEYGEKAGEGNPFRWIVDPIDGTKSFLKGVPFYSVL
                     IALEIEGVSRVGVVCFPALDEILYAADGLGAWCNGRRVHVSGVSKLKESVFCYTSWSA
                     FRTEKKVDVFERLHKECFFGRGWSDAYGYHMVATGRAEIMADPHIQLWDVAPFPPIFR
                     EAGGFFGSWDGKEGHTHGEALATNGEIKSKVMKLMRSV"
     gene            complement(2269..3588)
                     /locus_tag="VFQ23_12410"
     CDS             complement(2269..3588)
                     /locus_tag="VFQ23_12410"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET9907446.1"
                     /translation="MEKDHSNLLENSLDGLHLELLRLFASQAVALQTPLYLVGGVVRD
                     LLLGRAIKDFDLVVEGDITAFAESILKKYGGKILVHSRFGTAKWVLNESTLKRLDFPV
                     SRFPDFELSFDLVSARSETYTQPGALPTVKQSSIDDDLQRRDFTINAMALRLDGNHYG
                     KLLDPLGGEAALESGTIRVIHENSFIDDPTRIYRAVRYEGRYGFQMDDETLALIPNAK
                     AIIAELSAERIRHELDLILAEPNAVFMLARLAELDLLRPIHPALVFDAPANARLTNIQ
                     SFRALRRISPWNLDRGEGNKNALGWLLWLMPLSHNDIGSLNVRLQFTSDFLASLFAAS
                     TMFSNLSSFTEITPSQFVERVGLYPLNAVEAVYFIAQDEKIKDIFFKYLSEWRHVKPH
                     ITGDDLQKHGLEPGPKYGEILRRLRAAWLDGEISSTEQESTLLKELL"
     gene            3911..4216
                     /locus_tag="VFQ23_12415"
     CDS             3911..4216
                     /locus_tag="VFQ23_12415"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HET9907447.1"
                     /translation="MDIKQLTEEMHHFVRSKGWYADDSKRPQTPRNLAISLALEAAEI
                     LEHFQWNDQVKDKKELSSELADVALYLLQLASIEEIDLEKAILDKLEINKTRTWDQE"
     gene            4213..4740
                     /locus_tag="VFQ23_12420"
     CDS             4213..4740
                     /locus_tag="VFQ23_12420"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HET9907448.1"
                     /translation="MKDSVFGGSQPKEGSEAYAEAQELGRLLAEDGHTVLTGGYIGVM
                     EAVSRGAREAGGHVIGVTCEDIEAWRPIKANSWVMEEIRKKTLLERLHTLIHECDAAL
                     AMPGGAGTLTEISLLWNLMIVESLHRRPLILVGRGWQSTFDQFFQEFDSYTPAPQREI
                     LVFAQDVQTAVKLLK"
     gene            4806..6245
                     /gene="proS"
                     /locus_tag="VFQ23_12425"
     CDS             4806..6245
                     /gene="proS"
                     /locus_tag="VFQ23_12425"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9907449.1"
                     /translation="MAEEKLPTRAEDFAEWYNQLVVRADLADYAPVRGCMVVKPYGWA
                     LWENITSWLDAEFKKTGHVNAAFPTLIPLSFFEKEKEHVEGFSPQLAVVTHGGGEELE
                     EKLVVRPTSETIIGYMYSKWIKSWRDLPLLIVQWGSVMRWEIRTKLFLRTAEFYWHEG
                     HTAHVDAEEAKEEMYRILDIYEEFCLNEAAIPVFKGAKSETERFPGAQITTSIEAMMW
                     DKRALQSGTSHYFGQNFAKAFDIQYLAQNNTLQYCETTSWAISSRIIGAMIMVHGDDQ
                     GLVLPPRLAPIQAVVVPIFKNDAEKSKVMEVAERVFKELKDAGIRIKMDDRDNVSSGF
                     KFNDWELRGVPVRVEIGPKDVEKGSVALARRDKPGREGKTFVSQSGLASTVSGLLVEI
                     QDSLLQRATEYRDANIHEPKDYDDLKQIVTEGWAYAFWCESTECETKVKEDTKATTRN
                     IPLNQPEQDGTCVVCGKPSKRKVYFARSY"
     gene            6311..7108
                     /locus_tag="VFQ23_12430"
     CDS             6311..7108
                     /locus_tag="VFQ23_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907450.1"
                     /translation="MNLEYYQTQREFILAEIIEKLSTDERFVAGWLTGSLSRNEADSL
                     SDIDLTLVVSDKFSTSLCTRLEQVSAQTSPERLTLFSQFGTPALLHENNNNAPEGGTF
                     TFVLYAKSAIMIDWILVPQSVAIRPIQSRLLFEKTILPMEPAPQPEDFDLSKKSVAEQ
                     WAFFWMMVIITIKYIQRGDGVFAAEWIEHLHGLMYDIERRLNRDVWRYKRGSHSKLQS
                     TREKQLESIRELCRRMQGLESRVTEFTGLRPVTPSAEIEVFLTFAQK"
     gene            7109..7906
                     /locus_tag="VFQ23_12435"
     CDS             7109..7906
                     /locus_tag="VFQ23_12435"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET9907451.1"
                     /translation="MPAIDLARLKKQTAQLADLFDQPQLFLRQHRDVLDFYVNHSLRS
                     QGVAPSSVLPTYRTPTVVLRQIETELGQIAEKQPLQALELADALWDEGWLETRLLAAF
                     LLGRIPPQEERLLARLTAWTQAVRDPSVRSALLTTSLARLRKETPDLFLVLVKEWLHP
                     ARQRMWSNGIQALMPLISSPDFDNLPPIFEIIEPIIRAAPATLQFDLQELIVLLYEAS
                     PDETTYFIQQILKSTKSPMLAVALRRMSSEFPAELQSNLREMLYKKR"
     gene            7948..8454
                     /locus_tag="VFQ23_12440"
     CDS             7948..8454
                     /locus_tag="VFQ23_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907452.1"
                     /translation="MLVSRAVLGAILLFLGRELNFLFAGGMAALIAIELTPRLPPEWP
                     AWFNPAFILGLGVLAAAIPAINERAGYFLSGFLAGGYYLSEYYAPGVFEVTLLPFIVG
                     GVIGGLIMGIFTEWALMIVSCLVGALYVTDLFRLTPTARTLMQGGLFVLGALTQVILM
                     RMQRHSDR"
     gene            8482..>8745
                     /locus_tag="VFQ23_12445"
     CDS             8482..>8745
                     /locus_tag="VFQ23_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907453.1"
                     /translation="MVGLINILLGGALLLAGRKLFWLFVGAIGFVTGLQFATSFWQGP
                     DNLALVFGLIVGVIFALLAVFLQSIAIGIAGFFAGGYVLTALAG"
BASE COUNT         2270 a         2142 c         2151 g         2182 t
ORIGIN      
        1 ctttcttatt ccgggggatg gatcgtatgg aatccagcct gtttttgtcg aagaccttgc
       61 tgacctggct gttgagggtg tttacagcca gtccaattac atcatcgatg cggtcggacc
      121 cgatgcctat acgttcaaag aactcattca gcgcattggc aaaacgattg gagcagagcg
      181 acccattatc tctgtgccac cacggcttgc cttacttgcc gcgcagtttc taagtctctt
      241 tgtgggggat gtaatcctca ctccggaaga agtggatgga ctcatggcaa acctgcttgt
      301 ctcaaaagaa ccaggacgag gcaaaactgc attcaaagat tggctggagg caaataagga
      361 tatcgttggg aagaagtacg cgtctgaaat caagaagcat tatgaatgat gaaaaaatta
      421 accatcaaag tgtcatactc ttcgatggta tctgcaatct ctgtaatagc tcagtgttat
      481 ttattttgaa gtgggagaag tcttcgtttt atcagtttgc ctcgatacaa tcagaggctg
      541 ggaaagaatt attgaaatgg tgcgggttgc caatgggcta tagcgaagca atcgtaatga
      601 tcgataacgg aaagatatat ttagggtcta ctgccgcgtt aaaaattgga caagaactca
      661 aattcccctg gtcagtctta tcttcgatcg gcttattgtt tccgaaattc cttcgagatt
      721 gggtttatcg tcaaatttca caacacagat atcaatggtt tggtaaaaaa gatatatgca
      781 tgttgccaac ggaaggatta agatcgagat tcatatgaat aaaaacgtgg agcacctttg
      841 ctccacgttt caatctatcg gtacgattcc gttgcatttg gggctatcca tacaaccata
      901 ataccttttc cctccaccaa atccgttgcg gtgaatgcgc agaaccatca tctttccgca
      961 aacaggacac attggcacag agtcagattt cgtggttgct cgaaggggtc cggtcttctt
     1021 taccttcgtg atggccagct ggaataattc caccaggtca tcctgcagat atcgttcatt
     1081 ggaggggaca tgtacaagcg gtaggcctgc actcacaaaa agatcctcca taaattgatc
     1141 ggtgtcgcgc gtttcgttac gagagaccgg cttcaccaat tccaccccga tggccggttt
     1201 gaacccaggg gtttcgcaca gcaaaaagtc cacgtgcttg cgaaagattt tgttaaagaa
     1261 atgcacgttc tcattggggc gcacaatgta gaaaatgtcg ttcagcgcca ctttcgggca
     1321 gatcacgtaa tgccggcccg ccatttcctg taaagcattc aggagtgcca gttccggtgt
     1381 ggatagaaaa gtctcgcgca ggcgatacgg tagacgctcg gcaagctgat ccggcacctt
     1441 aaaattaatg gatttttgat ctggcagttt catatttcca ccttattata gcaggagacg
     1501 cctatacgct ccgcatcaat ttcatgactt tactcttgat ttcaccgttc gtagccaacg
     1561 cctcgccgtg tgtgtgtcct tctttcccat cccaggagcc aaaaaaaccg cccgcctccc
     1621 gaaaaatcgg cggaaatggc gctacatccc aaagttggat atgtgggtca gccatgattt
     1681 ctgcccgccc cgtcgccacc atgtggtatc cataggcgtc gctccagcct cgcccaaaga
     1741 aacattcctt gtgcaagcgc tcaaacacat ccaccttttt ttctgtgcgg aaggcagacc
     1801 agcttgtata acagaacaca gactctttaa gtttggacac acccgataca tggacccgtc
     1861 ttccattgca ccatgccccc agcccatctg cggcatacag gatttcatcc agggctggga
     1921 agcaaactac gccaactcgt gaaacacctt cgatctcaag ggcgatcaat actgaataaa
     1981 atggaacgcc cttgagaaag gattttgtgc catcgatggg atccacgatc cacctgaacg
     2041 gattcccttc gccggctttc tcgccatatt cctcgccgac cagcgcatga gtaggataat
     2101 tcttttcgat ctcgccgcgg ataaattcct cggtggcctg atctgcagca gtgacgggag
     2161 agtcatcctg cttcatatca tgctgaatgc ctttgttgta atagctaagc gtaatgcgcc
     2221 cggctcgata agcaagctgc cgggcaaaag taagatagga ctgaaggttc acaatagctc
     2281 ttttagtagt gtagattcct gctctgttga actgatctcc ccgtctaacc aagcagcacg
     2341 cagacgtcgg aggatttcgc cgtatttcgg accgggttcc aaaccatgtt tctgcaggtc
     2401 atcgccggtg atatgtggct tgacatgccg ccactcggaa agatatttga agaatatatc
     2461 ctttatcttt tcatcctgcg caataaaata gacagcttct acagcattga ggggatatag
     2521 accgactcgt tcaacaaatt gactgggagt tatttcagtg aaggaggaga gatttgaaaa
     2581 cattgtggac gccgcaaaca aggatgccag aaaatctgac gtgaattgca atcgaacatt
     2641 gagggatcca atatcattgt gggatagtgg catcagccaa agcagccagc ccagggcatt
     2701 tttgtttcct tcccctctat ccaaattcca gggagaaatt cgtcgtagcg cgcggaagga
     2761 ttgaatatta gtgagcctcg catttgcagg tgcatcaaag accagagccg ggtgaatcgg
     2821 cctgagcaaa tccagttcag caagccgcgc cagcataaat acggcgtttg gttccgcaag
     2881 gatcaaatcc agttcatgtc gtatgcgttc tgcagagagt tcagcaatga ttgcctttgc
     2941 attgggtatg agggccagtg tttcatcatc catctggaat ccgtagcgcc cttcataacg
     3001 gactgcacgg tagatacgcg tgggatcatc aataaatgaa ttttcatgaa taacgcgtat
     3061 cgttccactt tcgagagccg cctccccacc taatggatcc agcagcttgc cataatgatt
     3121 cccatctaat cgaagagcca tggcattaat cgtaaagtcg cggcgttgca agtcatcgtc
     3181 gatgctagat tgtttcactg tgggtaaggc gccgggctga gtgtatgtct cagagcgtgc
     3241 tgaaacgagg tcaaaggaaa gttcgaaatc cggaaatcgc gataccggaa aatcaagccg
     3301 tttcagcgtt gattcgttca aaacccattt ggccgttcca aaacgggaat gaaccaggat
     3361 cttcccgcca tactttttca aaatggattc agcaaaagcg gtgatatcgc cttccaccac
     3421 aagatcaaaa tccttgatgg cacgaccaag gagcagatca cgtacgacgc cgcctacaag
     3481 atagagagga gtctgcaggg caacagcctg gctggcaaac agacggagca attcgaggtg
     3541 caagccatcc agtgaatttt caagcaggtt gctatggtcc ttttccatcg gtgtatttta
     3601 gcagagaaaa tcgcactgca aaaaacacct gggatgatag tggtataatc tccctcacta
     3661 tctacaggat cacttcactt atgttgaacc cgttgccgtc accccgcaat catcaacctg
     3721 cctggtctgg ctgaggttca tttcatcatg tctgatggac ggttcccgga ctcggcgagc
     3781 cgtctttttg tttatctcac ctggaccctc atggttttgt cttgattaag tttaggttca
     3841 aagcaaaaga gaatctggtt gttaagtggt ttactttgcc acggaaacca ctaaggtctc
     3901 gactcgaaac atggatatca aacaacttac ggaagagatg catcatttcg tccggtcgaa
     3961 aggctggtac gcggacgaca gtaaacgtcc acaaacaccg cgtaatttgg cgatctcgtt
     4021 ggcattggaa gcggcagaaa ttctggagca ttttcaatgg aacgatcaag tcaaagataa
     4081 aaaagaatta tcctcagaat tggcagatgt ggcgttatat ttgctgcagt tagcctctat
     4141 tgaagagatt gatttggaaa aagcaattct ggacaagtta gaaattaata agactcgcac
     4201 atgggatcaa gaatgaaaga tagtgtattt ggtggctcac agcctaagga aggttcagaa
     4261 gcctacgcag aagcacagga gcttggcagg ttgttagcag aagacggaca taccgtgctc
     4321 accggtggct acattggcgt catggaggcg gtctcgcgcg gtgcgcgtga agcaggcgga
     4381 cacgtgatcg gcgtgacttg tgaagatatc gaagcctggc gcccgatcaa ggcaaactcc
     4441 tgggtcatgg aagaaatccg aaagaagact cttctcgaac gattgcacac gcttatccat
     4501 gaatgtgatg cggcgcttgc gatgccaggc ggtgcaggca cgcttacaga gatctctttg
     4561 ctttggaatt tgatgattgt ggagtcgttg caccggaggc cgttgatact ggtcgggcga
     4621 ggctggcagt ccacgttcga tcagttcttc caggagtttg actcttatac gcccgcacct
     4681 caacgcgaga ttttggtctt cgcgcaagat gtgcaaactg ccgtgaaatt attaaaataa
     4741 ttcagtggtt tcgatacgcc ttcggctact caaccaccga agtacatcaa caacaagagg
     4801 aaaaaatggc tgaagaaaaa ttacccaccc gcgcagaaga tttcgccgaa tggtacaacc
     4861 aactcgttgt ccgagcagac ctggcggatt acgcgcccgt gcgtggttgt atggtcgtaa
     4921 aaccctacgg ctgggcgctc tgggagaata ttacatcctg gttggatgca gaattcaaga
     4981 aaacgggtca cgtcaacgcg gcgttcccca ctctgattcc actttccttt tttgaaaagg
     5041 aaaaagaaca tgtggagggc ttttcgcccc aacttgctgt cgtcacacat ggaggtggcg
     5101 aagagcttga agagaaattg gtggtccgcc ccacatccga aaccatcatc ggatatatgt
     5161 actccaagtg gataaaatcc tggcgcgatt tacctttgct cattgtccaa tggggttcgg
     5221 tcatgcgctg ggagatacgc accaagctct tcctccgcac cgctgaattc tactggcacg
     5281 aaggtcacac agcacacgtg gacgcagaag aagccaaaga ggaaatgtat cgcatcctgg
     5341 atatctacga agagttttgc ctgaacgaag cggcgattcc tgttttcaaa ggcgccaaga
     5401 gtgaaaccga gcgcttccct ggcgcacaga tcacaacctc cattgaagct atgatgtggg
     5461 ataagcgcgc cctgcaatcg ggcacatcgc attacttcgg gcagaatttc gcgaaggcat
     5521 tcgatattca atatcttgcc caaaataata cattgcagta ttgcgagact acatcgtggg
     5581 cgatttcaag ccgtattatc ggggcgatga tcatggttca cggggatgac cagggactcg
     5641 ttctacctcc tcgccttgct cctattcagg ctgtcgtggt gcccattttc aagaacgatg
     5701 cagagaaaag caaagtgatg gaagtggctg agcgcgtctt caaggaactc aaggatgcag
     5761 gtatccgcat caaaatggat gatcgtgaca acgtcagctc gggattcaaa ttcaatgact
     5821 gggaactgcg cggcgtaccc gtccgtgtag agatcggtcc caaagacgtg gaaaaaggtt
     5881 cggtggcact tgcccgccgc gataagcctg ggcgggaagg caaaaccttc gtttcacaat
     5941 caggtctggc atcgactgtg agcggacttc tcgtcgagat ccaggattca ctcctccagc
     6001 gcgcgactga atatcgcgac gccaatatcc acgaacccaa agattacgat gacctcaagc
     6061 aaatcgttac agaaggctgg gcctatgcct tttggtgtga atccacagag tgcgaaacaa
     6121 aggtcaagga agataccaaa gccacgacgc gtaacattcc attgaaccaa cctgaacagg
     6181 atgggacgtg tgttgtgtgt gggaaacctt ccaaacgcaa agtatatttc gcgagatcct
     6241 attaaaagca ccgtgcgttt cagtgtcacg tgtcaggaaa tactggcacg tggcaccttt
     6301 ctgaatcaag atgaatttgg aatattatca aacgcagcga gaatttatac ttgctgagat
     6361 cattgagaaa ctgtcaacag atgagcgatt tgtcgccgga tggctgaccg gcagcctcag
     6421 tcgaaatgaa gcagattctc ttagcgacat cgatctgaca ttggttgtgt cagacaaatt
     6481 cagcacgagc ctttgcacgc ggctcgaaca agtcagcgcg cagacctctc cggagcgtct
     6541 cactcttttc agtcaatttg gaacccctgc tttacttcac gagaacaata ataatgcacc
     6601 ggagggtggg acgtttacat ttgtccttta cgcaaaatcg gcaatcatga tcgattggat
     6661 attggtccct caaagcgtag caatccgccc aattcaatca cgcctgttgt tcgaaaagac
     6721 aatcttgcca atggagccag cgccccagcc tgaggatttc gacctcagca aaaaatctgt
     6781 ggcagaacaa tgggcatttt tctggatgat ggtaattatc acgatcaaat acattcaacg
     6841 aggcgatggt gtatttgcag ctgaatggat tgagcatcta catggtctta tgtacgacat
     6901 cgaacgacga ctcaatagag acgtctggcg ctacaagcgt ggctcgcaca gcaaactaca
     6961 atcgacacgc gaaaaacagt tagaatcaat ccgtgaacta tgccgcagaa tgcaggggct
     7021 cgaatccagg gtaactgagt ttacaggtct tagacctgtc accccttcag cagaaatcga
     7081 agtgttcctc acatttgctc aaaaataaat gcccgctatt gatttggcac gtctaaaaaa
     7141 acaaaccgcc caattggctg atctattcga ccagccacag ctctttttgc gccagcaccg
     7201 cgacgttctg gatttttacg tcaaccattc cttgcggagc cagggggtgg ctccgtcgtc
     7261 agttcttccc acctatcgaa caccaactgt ggtgttgaga cagattgaaa cggagctggg
     7321 tcaaatcgca gaaaagcagc cgcttcaggc ccttgaatta gcggatgcct tatgggatga
     7381 aggctggctc gaaacacgac tgctcgccgc gtttctgtta ggacgcatcc ctccacagga
     7441 ggagcgcctg cttgcacgcc tcacggcctg gactcaagcc gtccgtgacc ccagcgtccg
     7501 ctcagccctc ctcacaacca gcctcgcccg acttcgcaaa gaaacaccag acctgttttt
     7561 agttctggtg aaggaatggc tgcatcctgc gcggcaacgg atgtggtcga atggcataca
     7621 agccctcatg cctctaatct cctcaccaga tttcgacaat ttacctccaa tcttcgaaat
     7681 catagaaccc ataattcgtg ctgcaccagc cacgttacaa ttcgacttgc aggaattgat
     7741 cgttttgctg tatgaagcct ccccagacga aacaacatac ttcattcagc aaattctcaa
     7801 aagcaccaag agtccaatgc tggctgtggc tctacgacgt atgtcgtctg aattcccggc
     7861 agaactccag tcgaatctgc gcgagatgct gtacaagaaa agataagttc caaagcagcg
     7921 tgtcttggat caggaaagga gaattgcatg ttggtttcca gagcagtcct tggagcaata
     7981 ttgctctttc ttggacgtga acttaatttt ctctttgcag gtggaatggc agctttgata
     8041 gcaatcgaac tcacgccgcg tcttccacct gaatggcccg cctggttcaa cccagcgttc
     8101 atccttgggc ttggagttct tgccgcggcg attcctgcga tcaatgaacg cgcgggttat
     8161 ttcctctccg gttttttagc gggcgggtat tatctttcag aatactatgc accgggtgtt
     8221 tttgaggtga cattattacc ttttattgtc gggggagtta tcggcgggct aatcatgggt
     8281 atctttaccg aatgggcact gatgatcgtt tcgtgcctgg tcggcgcact ctatgtaacc
     8341 gacttattca ggctaacgcc caccgcgcga accttaatgc agggaggctt attcgttctc
     8401 ggtgcactga ctcaggtcat cctgatgcgg atgcaaagac actctgatcg atagaacaat
     8461 gcatcatacc tggagatgga aatggtcggg ctgatcaaca tacttctcgg tggtgccttg
     8521 ctgcttgcag ggcgcaaact tttctggcta tttgtcggtg caattgggtt tgttaccggt
     8581 cttcaatttg caacaagttt ctggcaaggc ccggataatc tggctctcgt tttcggtttg
     8641 atcgtgggcg tgatcttcgc cttactggct gttttccttc agtccatcgc gatcggaata
     8701 gccggcttct tcgcgggcgg atatgtgctg acggcgctag caggt
//
LOCUS       DASTJP010000431        10429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1077112, whole genome shotgun sequence.
ACCESSION   DASTJP010000431 DASTJP010000000
VERSION     DASTJP010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077112"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VFQ23_12450"
     CDS             complement(<1..1236)
                     /locus_tag="VFQ23_12450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9907454.1"
                     /translation="MKASSQTHLPSLFSSLLFLLGALFFLSISLVTGVSALISLLTGE
                     TVQANLTILFLVLGFEGVLLLIATFVSILKFLNKQSAEQNIIFFLSVKQILLCIIVAG
                     ISIIFGSLISSNDSVNWIFLPILTIPAVVLPLWLLLSLTARNLSLGMRWQTWNVFGLG
                     MTLGPLILITLEVAVLIFGFLISIFYVVSQPELLSELERLSNELVILGPNSEDAANLV
                     APYLTRPGVILGALAYFALIIPALEEIFKPIGVWFLARKLDSQAQGFALGALSGAAFA
                     LAETFGVSGQTAEWGILLFTRIGTGILHITTSALMGAAIVLAWRERRYWQLLRNYILV
                     VSLHGVWNALALTSTFSTLMELFDQQGLLRNIQLPISIGMSLLAVGMFVFLIFYNRKM
                     RDVQSKIQGLGAAENLNSDP"
     gene            complement(1237..2457)
                     /locus_tag="VFQ23_12455"
     CDS             complement(1237..2457)
                     /locus_tag="VFQ23_12455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9907455.1"
                     /translation="MQKFSSFILLTFIITACAPAPAAPATTTSPTPNIIIPTPPSCTA
                     ISAEPTPGPDTPSVFPPQSETDRVRGSSNPTVTILDYSDYQDSKSGFFEEQLNQLLKE
                     HPEDVRVISRVFPLIAVNDKAALAAQAAEAAAEQNKFWEVHNLLFAQQQNWVDLSVQD
                     FEQWISAQVSALGMDVDQFKSDLKREDIVARIQKAWEDGQAMGLPGTPLVLINGQIYG
                     GPRDYSSLNDILQLLLLGKRQFASCPPLTVQRNRQYITTLHTEKGDVVIQLFPDKAPI
                     TVNSFLFLARNGWYDDITFHRVIPDLFAQTGDPSGTGKGNPGYYIITEIDPSLKFLGP
                     GMVAMVNSGPDTSGSQFFITYGAVPDYDGQFTIFGQVLRGMDVVQALTPRDAQPGTFT
                     PPGDTLIDITIEEK"
     gene            complement(2491..3780)
                     /locus_tag="VFQ23_12460"
     CDS             complement(2491..3780)
                     /locus_tag="VFQ23_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907456.1"
                     /translation="MFYRIARIVDFLALTLAIMIAAGGDTPRLTGESDQVRSFTRDIE
                     FDYPNWVWDATWLKIEQSTIYAPYVFERGTTKEIVFEYLRVTQRLMETEFSIEQIFAD
                     PNVADKNLASESLRAQRDELIARQNDLAPFAESTLQSQISQALADLGLTAVGQPLPPT
                     LYHVSSTPLALIVAPRDHIHQIANVSVLPTLTLDEQIKLEDDVAQKLNVSTLVVGIGG
                     VGVYPTMVTETTDLRWMLETIAHEWTHNYLNLRPLGLNYSTTPELRTMNETTASIAGN
                     EVGAYVLGEYYPEMLGSASSIALNPSQSLISFNPSDSSSNALDDPPPFDFRAEMHETR
                     VTADEMLAQGKIEEAEEYMEARRLVFWENGYLLRKLNQAYFAFHGAYADSPGGAAGED
                     PVGPAVRELRQQSASLEEFINTIAWMTSFEELQEAIK"
     gene            complement(3870..4517)
                     /locus_tag="VFQ23_12465"
     CDS             complement(3870..4517)
                     /locus_tag="VFQ23_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907457.1"
                     /translation="MNSKSKKIVFLFGVLIILFPILLAGNTLVTNENSSKLDRQEFRD
                     QMRKLWVDHSSLTRQFMISTVSNLPSQGEAAEHLNQNQNEIGALFALFYGEEAGDELK
                     KLLADHTAISTTLFQAAGKADVQVFEESIMLWYDNGVQIAEFLSDLNPENWPPKETRP
                     MLRTYLDLTLEQAVAYWNGYSSISGGAYQKVQDHAVTIADVLSEGIINRFRHRFK"
     gene            4854..6137
                     /locus_tag="VFQ23_12470"
     CDS             4854..6137
                     /locus_tag="VFQ23_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9907458.1"
                     /translation="MREFFRKNRSVLIILLIVGVAASAFLYVRGSRAETNTQFQTATI
                     ERGNLTATIGATGTVRAKQTAILIWQAAGTVDTVNVNAGDVVSANDELASLLKTSLPQ
                     SVILAEADLVDAQKTLDELLTSETSQAEAVIALREAQKAYDRAANWRKELNGRIHIKQ
                     IVYKKIGNRTIPTLKEFRGYADAETIAKADEDVALAKAKLDDAQRTLDNLTNGNSPEL
                     IAAQARVDAAQATLNLAHITAPFAGTVTESHSLAGDQVSAGATAFRLDNLTSLFVDVE
                     VSEVDINSVTVDQTVSLSFDAILDKDYHGKVVEVAKAGNDVNGVVSFTVTVELTDADE
                     QVKPGMTAAVNITVREMQDVILVPNRAVRLVDGSRVVYLLVDNQPVKTEISLGSSSDT
                     ASAIANGDISEGDVVILNPPVEFGPGGGPGGGFGN"
     gene            6148..6825
                     /locus_tag="VFQ23_12475"
     CDS             6148..6825
                     /locus_tag="VFQ23_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907459.1"
                     /translation="MENVIEARDLRKVYQMGEVQVEALRGVSFEIKRGEVIAIMGPSG
                     SGKSTLMNTLGCLDRPSSGEYFLDGEPVASLNDDQLASIRNRKVGFVFQSFNLLSRQT
                     AITNVELPLRYSGAMEGRRARAIEALKAVGLGERLTHRPTELSGGQQQRVAIARAIVN
                     TPAIIMADEPTGNLDSKVGKEIMNLLLNLNKDSGTTLIIVTHDPNIADQTQRVIRLRD
                     GLLESAS"
     gene            6822..8051
                     /locus_tag="VFQ23_12480"
     CDS             6822..8051
                     /locus_tag="VFQ23_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9907460.1"
                     /translation="MTLAQAFLEALESLSGNKLRSGLTVLGIVIGVAAVIAMLAVGRG
                     AEASITGSISGIGTNLLFVFRGGQDASVRNERPITLGDADAIADPFAAPSVEAVAPIL
                     QSNRELSYGGERTTVEVNGVTPAYFAVQNYTLTEGEFINEEHLLGRASVVLIGPEAAD
                     TLFGRHDGITGETIRIEGQPFRILGVLASKGGGLFGSQDNVVAIPFSTAQARLIRRNT
                     RDQVDLLLVQAVSGDAVLMATEEIAQILRQRHRTPVGADDFTIFTQQDLLTTFQSITG
                     ILTIFLGGIAGISLLVGGIGIMNIMLVSVTERTREIGLRKALGARRRDILFQFLTESS
                     LLSLIGGIIGIMFGWLISFAVGQIAVASGTAFSPVVGYDAVALATIFSAAVGLFFGIY
                     PANRAASLEPVEALRYE"
     gene            8111..9052
                     /locus_tag="VFQ23_12485"
     CDS             8111..9052
                     /locus_tag="VFQ23_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HET9907461.1"
                     /translation="MSDFNILITDGLDQSGQSILRATTKVEDQSGISADDLVKIIPNY
                     DALIVRGRTKVTPSVMDVASRLKVIGRAGVGVDNIDLEAAKKHNITVVNAPMSTSLAV
                     AELTFGLLLALARELPRADAGMKAEQWLKKELEGVELNGKTLGVIGFGRIGVEVGKRA
                     GAFGMNVIAYDPLISEDEIQQRGAEPVSIQDLYAWSDFISLHLPLNVQTRDMVGPLAF
                     SQMKNGVRIVSAARGGIIAESALLDALNSGKVAGAALDVFEQEPPGLTEVVAHPRVIA
                     TPHIGAQTAEAQSRASEDIANEVLSALHNKPLRWKVN"
     gene            9142..10050
                     /locus_tag="VFQ23_12490"
     CDS             9142..10050
                     /locus_tag="VFQ23_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907462.1"
                     /translation="MYRNFIFIIFLLALLAACAAPTASPLPTPYPPEFLPTVIAQTAE
                     AASVEGTQTSIALTPTGIPTRTRFPTFTPTIPPSPTVTDFPWHKPAAIEIQAPGPMSR
                     VISPINLRMDIVAGESEKVQIDLYGEDGRLLSRTVKTVRTSREGVPQNIRIPFEIRAT
                     AEVGRITVSTQDEAGRMQALNSVRVLMLSSGANEINPAGNPAEPIGVFSPLPEEAVSG
                     GVLNVKGDLWPFNLQPVILELVGPDGRSIGLRILTVEHINPQLFETTIPYKVDEPTLA
                     RLTIYQDDDRINGLFYVYSQEVLLNP"
     gene            10193..10266
                     /locus_tag="VFQ23_12495"
     tRNA            10193..10266
                     /locus_tag="VFQ23_12495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10227..10229,aa:Pro,seq:cgg)
BASE COUNT         2607 a         2526 c         2720 g         2576 t
ORIGIN      
        1 aggatcgctg ttcaggtttt cagcggcacc aagtccttga atcttagact gtacgtctct
       61 catctttcga ttgtaaaaaa ttagaaatac aaacattcca actgccagaa gactcatccc
      121 aattgagatc ggcaattgta tatttcgcaa gagaccttgt tgatcgaaca attccatgag
      181 ggtggaaaat gtagaagtaa gcgccagggc gttccagaca ccatgcagtg aaacaaccag
      241 gatatagttt cggagcaatt gccaatagcg acgttcacgc caagcaagga caatcgctgc
      301 acccatcagt gctgaggtcg tgatatgcag aatccctgtg ccaatgcgcg taaaaagtag
      361 aatcccccat tcagcagttt gcccgcttac tccaaacgtt tctgccagtg caaaggctgc
      421 tccgctcagt gcaccgagag caaatccctg agcctgcgag tccaattttc gggccaaaaa
      481 ccagaccccg attggtttga atatctcctc cagcgccggg atgatcaacg caaaatatgc
      541 cagagcccca agaatgactc caggacgggt cagatatggg gcaaccagat tcgccgcatc
      601 ttctgaattt ggcccgagga tcacaagttc attcgacaac cgttcaagtt cgctcaacag
      661 ctccggttgc gaaacaacat aaaagatcga aatcaggaat ccgaagatca ggactgcgac
      721 ttccagcgtg atcaagatca atggaccgag agtcattcca agaccgaaga cgttccacgt
      781 ctgccagcgc atgccgagcg agaggttccg tgctgtcaga ctgagcaaca gccacaaggg
      841 aagtaccacc gccggaattg tgagtatggg gagaaagatc cagtttacgg agtcgttgct
      901 actgatcagg cttccaaaaa tgatcgaaat accagctacg ataatgcaga gaaggatttg
      961 ttttactgag aggaaaaata taatattttg ttcagcagac tgtttgttga gaaactttag
     1021 tattgataca aaagtcgcaa tcaacaacaa tactccttca aatcctaaga cgagaaaaag
     1081 tattgtcaga tttgcctgga ccgtttcgcc tgttagcaac gaaatcagtg cagagacacc
     1141 cgtaaccaaa ctaatactta ggaagaacaa agctccaagg agaaacaata atgagctaaa
     1201 tagagaaggg agatgtgtct gtgaagatgc tttcatttat ttttcttcaa ttgtgatatc
     1261 tatcaacgta tccccgggtg gcgtaaatgt tccaggttgt gcatcgcgcg gcgtcagggc
     1321 ttgcaccacg tccatgccac gtagcacctg cccgaagatt gtgaattgcc cgtcataatc
     1381 tggaactgca ccgtatgtga taaaaaattg actcccgctg gtatctggcc ctgagttgac
     1441 catcgcaacc attccgggtc cgaggaattt cagagatgga tcgatttcgg taataatgta
     1501 atagcctggg ttgcctttgc ctgttccgct cgggtcgccg gtttgcgcaa agagatcagg
     1561 aatcacgcga tgaaacgtga tatcgtcata ccagccattg cgagcaagaa acaggaacga
     1621 gttcacggtg atcggtgctt tatcggggaa caattgaatg actacatcac ctttctctgt
     1681 atgcaaggta gtaatgtatt gtcggttacg ttgaacggtc agtggtgggc acgatgcgaa
     1741 ttgacgttta cccagaagca gtaattgtaa aatatcgttg agactgctat agtctcttgg
     1801 gccaccataa atttgtccgt tgatgaggac cagaggtgtg cccggcagac ccatcgcctg
     1861 cccatcttcc catgcctttt ggattctcgc gacgatatct tcgcgtttca ggtcggactt
     1921 gaactgatcc acgtccatgc ccaaggcgga gacctgcgcg ctgatccatt gttcaaagtc
     1981 ctgcacggat agatctaccc aattctgttg ctgagcgaaa agcaagttgt gaacttccca
     2041 gaatttattt tgttccgcag cggcctcggc agcctgggcg gccagcgcag ccttatcgtt
     2101 gacggcaatc aaaggaaata cacgacttat cacgcgtaca tcctctggat gttctttcag
     2161 taattgattc aattgctctt caaagaatcc gcttttcgaa tcctgataat cactgtagtc
     2221 caggatggta acggttgggt tggatgagcc tcgcacccga tctgtctcgc tttgcggcgg
     2281 aaaaaccgac ggtgtatcgg gacccggtgt tggttcagca ctaatcgctg tacacgaagg
     2341 tggcgttgga atgatgatgt ttggagtggg agaagtcgtc gtggctggtg ccgccggcgc
     2401 gggcgcacag gcagtaatga taaaagtcaa cagaataaac gaagaaaatt tttgcataac
     2461 ggctttatta agcagaactc cccatctttg ttatttgatt gcctcttgaa gctcctcaaa
     2521 agacgtcatc caggcgatcg tattgatgaa ctcttccagc gaagcacttt gctggcgcag
     2581 ctcacggaca gcaggaccga ccgggtcttc accggctgcg ccgcctgggg aatctgcata
     2641 agcaccatga aaggcaaagt acgcctggtt gagcttgcgg agcaaatatc cgttttccca
     2701 gaagactagc cggcgtgcct ccatatattc ttcagcttct tcaatttttc cctgcgccag
     2761 catttcatcg gctgtgacgc gagtttcatg catttcggcg cggaaatcga acgggggtgg
     2821 atcgtccagc gcgttggaag aagagtcgga tggattaaac gagatcaggc tttggcttgg
     2881 gttgagcgcg attgaagagg cagagccaag catctccggg taatattctc ccagtacata
     2941 agctccgact tcatttcctg caatggatgc ggtggtctcg ttcatcgtgc gtagttcggg
     3001 agttgtgctg taattgagtc ccagcggacg gagatttaag tagttatgag tccattcgtg
     3061 tgcgatcgtt tcaagcatcc agcgcagatc ggtggtttcc gtcaccatgg tgggataaac
     3121 gcctacaccg ccgattccaa caaccagggt ggagacgttc agtttttgcg ccacatcatc
     3181 ttcgagcttg atttgctcgt cgagtgtgag agtcggcagg accgaaacgt tcgcgatctg
     3241 atggatatga tctcgcggag caacaatcaa cgcgagtgga gttgatgaaa catgatacag
     3301 agtgggaggc agcggctgac ctaccgctgt gaggcctaaa tctgcaagcg cctgactgat
     3361 ctgcgactgt aacgtcgatt cagcgaaagg agccaaatca ttctgccggg caatgagctc
     3421 gtcgcgttgg gcgcgaagtg actcactggc taaatttttg tcagcgacat ttggatccgc
     3481 aaagatttgt tcgatgctaa attccgtttc catgagccgt tgagtcaccc gtagatactc
     3541 gaacacgatc tccttggtgg tgccacgttc gaagacgtat ggagcataga tcgtgctttg
     3601 ttcaattttc aaccaggttg catcccaaac ccagtttggg tagtcgaatt cgatatctcg
     3661 tgtaaaggat cggacctgat ctgactcacc ggtcagcctt ggtgtatctc ccccagccgc
     3721 gatcataatc gccagggtca gtgccaggaa atcaacgatg cgcgcaatgc gatagaacat
     3781 tcgaagatta taatcagaaa cgggaaccag gagatggaac ctctcagttc ccgttaatcc
     3841 atcatcagtc tgatgtgtgg ctgggatact tacttaaatc gatgccggaa tctattgata
     3901 attccttcac tcaagacatc ggcgatcgta accgcatgat cctgaacctt ctgatatgca
     3961 ccaccactga tgctggaata gccattccaa taagccacag cctgttcaag ggtcagatca
     4021 agatatgttc tcaacatagg tctcgtttcc ttgggtggcc agttttccgg attcaggtct
     4081 gacagaaatt cagcgatctg aactccattg tcataccaaa gcatgatgga ctcctcaaat
     4141 acctgaacat ccgctttccc ggcggcctgg aacagggtgg tactgatcgc tgtatggtca
     4201 gcgagcaatt tttttagctc gtccccggct tcctctccat aaaagagagc gaacagggct
     4261 ccaatttcat tttggttttg gttcaggtgt tccgccgcct cgccttgtga aggaagattc
     4321 gaaactgtgc taatcatgaa ctgtcgtgtc aaactgctgt gatctaccca aagctttcgc
     4381 atttgatcac gaaattcctg ccgatccaat ttggatgagt tttcgtttgt aactaaggta
     4441 ttaccagcga gcaggatggg gaacaaaata atcagaactc caaataaaaa aacaatcttc
     4501 ttgctcttgc tattcatctt ttctcctcag atttgatgaa gttttgggct cttcaattta
     4561 aattaaaaaa tctattaact ccttaacgaa cattgatgat gttttggctg gcgatgatga
     4621 actgttgctg gattgtgcta ctatctataa cgaatatcaa caagtttttg tttaactgag
     4681 ttttcgcgga attcccgccg tagaccgaaa gtcggtgatg agtgaacagg aaagcagtcg
     4741 ccagttgata attttgttaa ttttacaaaa ccctgacgca aaaattcatt gccaattcat
     4801 atttgtagtg tagaattccg ccgttaatga aaatagccct caaaggagga ttaatgcgtg
     4861 agtttttcag gaaaaatcga agtgttctga ttatactgct gattgttggg gttgcggcgt
     4921 cagcatttct ctatgtacgc ggttcaagag ccgaaacgaa cacccagttc cagacggcca
     4981 ccatcgaacg tggcaatttg accgcgacca tcggtgccac cggaactgtg cgcgccaaac
     5041 aaacggcaat actcatctgg caggcggcag gaaccgtgga tacggtcaat gtaaacgctg
     5101 gtgatgttgt cagtgcaaat gatgaactgg catcgttgtt aaaaacatcc ctgccccaaa
     5161 gtgtgatact ggcagaggca gatcttgtgg atgcacaaaa aacgctggat gaactgctca
     5221 cctctgaaac gtcacaggcc gaagcggtaa tcgccttacg tgaggcgcaa aaagcttatg
     5281 atcgggcggc aaactggcgc aaggaattga atgggcgtat ccatatcaaa cagatcgtct
     5341 ataaaaagat tggcaatcgg accattccca cgcttaagga atttcgtgga tatgccgacg
     5401 cggaaacaat cgcgaaagcg gatgaagacg tggccctggc gaaggcaaaa ctggatgacg
     5461 cccagcgcac tctggataac ctaacgaacg gtaactcacc tgagcttatc gctgcacagg
     5521 ctcgtgtgga tgcagctcag gcgacactca acctggcgca cattactgct ccgttcgccg
     5581 gcaccgtgac ggaatctcat tctctagctg gtgatcaggt aagtgcagga gcaacagcct
     5641 ttcgcctgga taatctcacc agtctgtttg tggatgtgga agtttcagaa gtggatatca
     5701 atagcgtaac tgtggatcaa actgtttcgc tttcttttga cgccatcctt gacaaggatt
     5761 atcacgggaa agttgttgaa gtggcgaaag ccggtaatga tgtcaatggt gttgtcagct
     5821 ttacggtgac agtggaactg actgatgcag atgagcaggt aaagcctggt atgacagcgg
     5881 ctgtgaacat cactgtcaga gagatgcagg atgtcattct tgttccaaac cgggccgtgc
     5941 gcctagtgga tggatctcgt gttgtatatc ttttggtcga taaccaaccg gtcaaaacag
     6001 agatcagctt gggttcttct tctgatacgg cgagcgccat tgccaatgga gatatcagtg
     6061 aaggcgatgt tgtgattcta aatccgccag ttgaattcgg accaggcgga ggtcccggcg
     6121 gtggcttcgg gaactagaaa tatgtctatg gagaatgtta tcgaagcccg cgatcttcgc
     6181 aaggtctatc aaatgggcga agtacaagtg gaagcgctgc ggggcgtatc gtttgaaatt
     6241 aaacgcggcg aagtcatcgc cattatgggc ccttccgggt cgggcaaatc cacactgatg
     6301 aatacgctgg gctgtttgga tcgacccagc tccggcgaat atttccttga cggagagccg
     6361 gttgcttcct tgaatgatga ccagcttgcg agtattcgca atcgcaaagt tgggttcgtc
     6421 tttcaaagtt tcaacctcct gtcgcggcag acagccatta ccaatgtgga actgccgctg
     6481 cgttattccg gcgccatgga gggacgtcgg gcgcgcgcca tcgaagcatt aaaggcggtt
     6541 ggccttggcg agcgtctgac acatcgtccc acagaattat cgggcggaca gcaacaacgg
     6601 gttgcgattg cacgcgccat tgtcaatacc ccggcgatca tcatggcgga tgaacctacc
     6661 ggcaaccttg attcaaaagt aggcaaagag atcatgaatc tcctgttgaa cctcaacaag
     6721 gacagcggta cgaccctaat catcgtaacg catgacccaa acatcgcgga tcaaacgcag
     6781 cgcgtaattc gcttgcgaga tggcttactg gagagtgcct catgactctg gcacaggcat
     6841 ttcttgaagc gctcgaaagt ttgagtggaa acaaactgag atctgggctg accgtgcttg
     6901 gtatcgtgat cggtgtggca gcagtgatcg ccatgctggc agtggggcgc ggtgcagaag
     6961 catccattac gggctccatc agtggaattg gaacgaatct cctgtttgtg tttcgcggtg
     7021 gacaggacgc ctcagttcgt aatgaacgcc cgatcacgct gggtgatgcc gatgcaattg
     7081 ctgatccgtt tgctgcccct tctgtggagg ccgttgcccc gatcttgcaa tcaaatcgtg
     7141 aattatccta tggtggagag cgcacaacag tagaggtgaa cggtgtgacg ccggcttatt
     7201 tcgctgtgca gaactataca ttgaccgaag gcgagtttat caatgaagag catctactgg
     7261 gacgcgcatc cgttgtgttg atcggaccgg aggctgccga tacgttattc ggccgccacg
     7321 atggcatcac gggcgaaacg atacggatcg aaggccagcc attccgaatc ctgggcgtcc
     7381 ttgcctcgaa aggcgggggc ttgttcggaa gtcaggataa cgtcgtcgcg attccattca
     7441 gcaccgcgca agcacgcctg attcgccgca atacgcgcga ccaggttgac ctcctgctgg
     7501 tccaggctgt aagtggagat gctgtgctga tggcaaccga ggaaatcgca caaatccttc
     7561 gccagcgaca tcggactccc gttggtgcag atgatttcac catttttacg caacaggacc
     7621 tgctaacgac ctttcaatcg attaccggga ttttgacaat tttcctgggt ggcatagcag
     7681 gtatttcgct tttagtgggt ggcattggta ttatgaatat catgcttgtg tctgtgaccg
     7741 agcggacgcg cgagatcggt ttgcgtaagg ccctgggggc gcggcgccga gacatcctct
     7801 ttcaatttct aaccgaatct tcattattaa gcctgattgg tggcatcatt gggatcatgt
     7861 ttggctggct catttccttc gccgtgggtc aaattgccgt ggcaagtgga actgcctttt
     7921 cgccggtcgt cggttatgat gcagttgctc ttgctaccat attctcggcg gcagtcggac
     7981 tcttctttgg catttatcca gcaaatcgtg cagcatcgct ggaacctgtt gaagccttga
     8041 ggtatgaata atatttgcat ataggggatg gccacgtccg tcccctacat gcccatgata
     8101 aaatatttcc atgtctgatt tcaacattct tattacagat ggactggacc agagcggaca
     8161 gtccatcttg cgtgctacta caaaggtgga ggatcaaagc ggcatctctg ccgatgatct
     8221 ggtcaaaata attcccaatt acgatgcgct catcgttcgg ggccggacga aggtgacacc
     8281 ttccgttatg gacgtggcct cgcggctgaa agtgatcggt cgtgcgggcg tcggtgtgga
     8341 taacattgac ctcgaagccg caaagaaaca taacatcact gtggtcaatg caccaatgtc
     8401 cacctcgctt gcggtcgcag aattgacgtt tggtcttttg ctggcgcttg cgcgtgagct
     8461 ccctcgtgcc gatgcaggga tgaaagcaga gcaatggctc aagaaggaac tggaaggcgt
     8521 cgaattaaac ggaaagacgt tgggtgtgat cggctttggt cgaatcggtg tggaagtggg
     8581 taaacgcgcc ggcgcgtttg gaatgaatgt gatcgcgtat gacccattga tctcagaaga
     8641 tgaaattcag cagcgcggcg cagagccggt ttcgatccag gacctgtatg catggtccga
     8701 cttcatttca ctccatttgc cgctcaacgt tcaaacgcgc gatatggtgg gtccattggc
     8761 tttctctcag atgaagaatg gcgtgcgaat tgtttccgcg gcacgcggtg gcattattgc
     8821 cgagtctgcg ctgctggatg ctctgaatag cggaaaggta gcaggtgccg cgttggatgt
     8881 cttcgagcag gaaccacctg gtttaacgga ggttgttgca catccacgcg tgattgccac
     8941 tccacatatc ggagcgcaga ccgcagaagc acaatcccgt gcctcggaag acatcgcgaa
     9001 cgaagtgtta tctgcattac acaataaacc gttgcgctgg aaagtaaatt aagatgtcat
     9061 tgtgagcctg agcaagtggt gaggcaatcg cctgataagt tgagattgct tcccgtaggc
     9121 tcgtaatgac atctccaaat catgtatcgc aatttcattt ttatcatttt tcttctcgca
     9181 ttattagctg cctgcgccgc gccgacggca agccctctcc caacacctta cccgcctgag
     9241 tttctgccga ccgtgatcgc gcagactgcc gaggccgcaa gcgttgaagg gacgcaaacc
     9301 tctattgccc tcacaccaac gggcataccc acgcggacgc gcttcccgac ctttacgccc
     9361 actataccgc cgtcccctac ggtaaccgat tttccctggc ataagccagc cgcaatcgag
     9421 atccaggcac caggaccgat gtccagagtg atttcgccga tcaatcttcg catggacatt
     9481 gtcgccggcg aaagtgaaaa agtgcagatc gatctgtacg gcgaagatgg tcgtttgctg
     9541 tcgcgcacag taaagaccgt gcgcacttcc cgagaaggcg ttcctcaaaa catccgcatt
     9601 ccttttgaga ttcgagccac tgctgaagtg ggacgaatca ccgtcagcac acaagatgag
     9661 gctggcagga tgcaggcttt gaactcggtg cgtgtgctga tgctttcgag cggggcaaat
     9721 gagatcaacc cggctggcaa tccggctgag ccgatcggtg tgttcagtcc cctgccagag
     9781 gaagccgttt cgggcggtgt gttgaatgta aaaggagatt tgtggccgtt caacctccag
     9841 cccgtgatcc ttgaactggt agggcccgat ggcagatcga tcgggctgcg aatccttacc
     9901 gtggaacata tcaacccaca attgttcgaa acgaccattc cttataaggt cgatgaaccc
     9961 acgcttgcac gcctgaccat ttatcaggat gatgatcgta tcaacggatt gttttacgtc
    10021 tatagtcagg aagtgctgct aaacccataa tttttaaaca cgaaagacac aacggtcgcg
    10081 aagaaaaacc tttagatatt tatcttagtg ttaccttagt gggcgtcgtg tttaggattc
    10141 tttgagcatg cttgcaaaat gaaagtgcgg acgttataat tcgcaggcta ttcggggtgt
    10201 ggctcagacc ggtagagcgc acggttcggg tccgtgaggt cccgagttca aatctcggca
    10261 ccccgacagt agtccacatg gttagcctaa aaggctaacc atgtggactt aaataggctg
    10321 cgaatcaggg ggcttgaaac tgcggtccac atggctctta tccgtgacga ctgaagctca
    10381 tctggtccgg ccagaatcat agaccttacg gcttgcccat aaaactaac
//
LOCUS       DASTJP010000432         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1381670, whole genome shotgun sequence.
ACCESSION   DASTJP010000432 DASTJP010000000
VERSION     DASTJP010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381670"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2438
                     /locus_tag="VFQ23_12500"
     CDS             <1..>2438
                     /locus_tag="VFQ23_12500"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907463.1"
                     /translation="GAMNNKELRRAIEEPAQRGHWEIEPGLVDVLLRDVGQEPGALPL
                     LSHALMETWQRRRGRTMTLGGYASSGGVHGAIAETAETVFTDRFTHEQQAIARRIFLR
                     LTELGDETSAVDTRRRAKLDELILRPEEGDITRAVLKSLADARLITLDENSAEVAHEA
                     LIREWPALRGWLEENRDGLRLQRQLTEAAQAWQELDHATDLLYRGARLDQFESWSQET
                     ELALTQLEIDFLQASLDYERERERRAVEQKRTQRNLQRSFVGTLIIGLIVSIVLSVMA
                     LAARNNATRSAAEFRSIALASGAQEELDEGFPDRALALALEANNMEHPPPRAQQMLFT
                     AATSTWMRQRFEHGAAIRRVAYSPDGKHLLTTGENHTAALWDIETGQVVKSFEHNETI
                     WGSAFQPGGDLIAMAGYDGRIILWNPVLDEISYLNVSDTFLRDIIFSADGNRILAGGE
                     DALITLWDVETRSLVRTYEGHTSIVYGLDFNSDESLFISGSNDNTARIWDIATGQTLM
                     VLEMPEDEELSDERGVFGVQFLPDSQRVVTGDLGGFLYLWDITTGEVLRQYEDVDGVI
                     YPVALNNDGEMVLMGTSSGEAYINLYDVENGKLLRSYFGHTIGIYSLAFSPDGKTIAS
                     GSIDGTAAIWDLGVPGVLYKVELPASGSGSEIAMHPDGRSFFEVGFDGIIRMREVATG
                     QIIRTFDDNAGRWSLSVAVSPDGRYVISGDYVTAEPFDEQNVYLWDIQTGELLHTFDQ
                     NFGWIFTIAFSPDGQKVAVGETEGKRILMYEVSTGRLLQTFEGHTNWVQNMVFNPDGQ
                     KLYSAGRDLTIREW"
BASE COUNT          595 a          557 c          699 g          587 t
ORIGIN      
        1 ttggcgcgat gaataacaag gaactgcgcc gtgccattga ggaacctgcc cagcgtgggc
       61 attgggaaat cgagcctggg cttgtcgatg ttctgttgcg cgatgttgga caggaaccgg
      121 gggcgctgcc tttgctgtcc catgcgctca tggagacatg gcagagacgc cgtggtcgca
      181 cgatgaccct gggtggttat gcttcgtcag gcggcgtgca tggggcaatc gcggagacgg
      241 ccgagaccgt cttcacggat cggttcaccc atgaacagca agcgattgca cgcaggatct
      301 ttctgcgatt aacggaactc ggtgatgaaa cttctgctgt cgatacccgg cgacgggcaa
      361 aacttgatga actgatcttg agaccggagg aaggtgacat cacgcgcgct gtgttgaagt
      421 ctttggctga tgcacgcttg attactctcg atgagaattc agccgaggtg gcacacgagg
      481 ctctaattcg tgagtggcct gcgctacgag gttggctgga ggaaaaccgc gatggtttac
      541 gcctgcaaag acagttgacc gaagccgcgc aggcgtggca ggaattggac cacgcaacag
      601 atctacttta tcgcggcgcg cggcttgatc aatttgagtc ctggagtcaa gaaacagaat
      661 tagccctgac acaattggag atcgattttt tacaagcaag cctggattat gaaagggaac
      721 gtgaaagaag agcagtagag caaaaacgga ctcaacgtaa cttacaacga tcttttgtgg
      781 gaacgttgat tatcggactt atcgtttcta ttgtgctttc tgttatggca cttgctgcac
      841 ggaacaatgc aaccagaagc gctgcagaat tcaggagcat agcgctggca tccggcgctc
      901 aggaagagtt ggacgagggg ttcccggacc gagcattggc gctggcactc gaagccaata
      961 atatggaaca tccacccccg agagcacaac agatgctctt taccgcggct acatccacct
     1021 ggatgcgaca acgcttcgaa catggcgcag ccattcgcag ggtggcttac agccccgatg
     1081 gaaaacattt gcttacaacc ggtgaaaatc atacagccgc tctctgggat attgaaacag
     1141 gccaggtcgt gaagtccttc gaacataatg aaacgatttg gggttctgcg ttccagcccg
     1201 ggggtgatct tattgccatg gctggctatg acggacgtat cattctatgg aacccggtcc
     1261 tggatgagat aagctacctt aacgtgagtg atacgtttct gagagatatt atcttttcgg
     1321 cagatggaaa tcggattctg gccggcgggg aagatgctct tatcactctg tgggatgtgg
     1381 aaaccaggag cttggtccgt acctatgaag ggcacaccag cattgtgtat gggctggact
     1441 ttaactcaga tgaatcactt ttcatttccg gttcaaatga caacacggca cgcatttggg
     1501 atatagccac aggccaaacc ctgatggttc tagagatgcc agaagatgaa gaattatcgg
     1561 atgagcgagg ggtgtttggt gtccagtttt taccggacag ccaacgtgtc gtcaccggtg
     1621 atttgggtgg ttttctgtat ctatgggata tcacaaccgg agaagttctc cggcaatatg
     1681 aagacgtcga cggggtaata taccctgttg cgctcaataa tgatggcgaa atggttctta
     1741 tgggcacatc cagtggtgag gcgtatataa atttgtacga tgtagaaaat ggaaaattgc
     1801 ttcgctccta tttcggccac acaatcggta tctatagtct tgctttcagc ccggatggga
     1861 aaacaattgc ctcgggctca attgatggaa ctgcagcaat ctgggacctg ggggtccccg
     1921 gtgtcctcta caaagttgag ctccccgcat ccggcagtgg ttcggagatc gccatgcacc
     1981 cggatgggag atcatttttt gaggttggct ttgatggaat catccgcatg agggaagttg
     2041 ccactggtca aatcatccgt acgtttgatg ataatgccgg gcgctggtct ttgagtgtgg
     2101 cggtcagccc ggatgggcgc tatgttatct cgggcgatta tgtgaccgcc gaaccatttg
     2161 atgaacaaaa cgtttatctg tgggacattc agacaggtga acttctgcac acattcgacc
     2221 agaatttcgg gtggatcttc acaatagctt tcagccccga tgggcagaaa gttgcggttg
     2281 gagagacgga aggcaaacgt atattgatgt acgaggtctc aactggtcgc ttactgcaaa
     2341 ctttcgaagg gcataccaac tgggttcaaa atatggtatt caacccggac ggccagaagt
     2401 tgtattctgc cggacgtgac ctgaccatcc gtgaatgg
//
LOCUS       DASTJP010000433         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1105359, whole genome shotgun sequence.
ACCESSION   DASTJP010000433 DASTJP010000000
VERSION     DASTJP010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105359"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2596
                     /locus_tag="VFQ23_12505"
     CDS             <1..2596
                     /locus_tag="VFQ23_12505"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9907464.1"
                     /translation="LYAQNHHQHLSDETAREFVDQTSGWITGMVLSNMPDMPRVSGID
                     TFAYLGRQVLDQQPAYVREFLMRTSLSDEFSADFCETTLGPLYAQRQNWYELMSLILE
                     KNLFVLPLGSDGRWLRYHHLFRDFLQTRLREERPHEVTPILERMVQAYENAGEWEKAY
                     FTCKQLNDLDALAGVVERAGTHMLQNARITLEGWINSLPPSLLRTRPGLISLRGPILV
                     AKGNLQEANNILDKAVTIYRKKHDIPGLTLALIRRANTLRFLGNYSASILDTEEALQL
                     AESDTAFQSLYAEALRLKGLNLHRMGESRHAVESLEHSLSLYSALNETDTIPTVLMET
                     GMAHHAVGDIDSARSSYQEALKLRRAEKNVYSQAEILNNLAVLYHQLGEYELASETFE
                     DGLVCARKSRNQRTESLILAGLGDLYSEIEEFDAALQAYQQAKAIASELDASFISNYL
                     IIAKGILAILREDWKSATQILTTHKRGIKVSQSVYERGLWSLFEGRYYLLKGEARKAI
                     RILQECKNFFTQDGRDLEIQWSMIWLGAAFEQAGQRNLARAEFKELLSVIVSPDHAFL
                     VAFRPAAEWVMDLQNDTEIGHQVKALLEKSQRLALRLPSIRRMVRRHTQLVQMPSASI
                     IIRTFGRGEVSVNGQDITISDWRTKSVRDLFFYFLFKQDSLTKEQIAEVLWPDVSDPA
                     SIKKRFKDAIYWIRRAVGKNVIVFDQEYYRLNRTLDYEYDVEAFESYLRRARKNKDRT
                     ERINWYQKATNLVRGPYLADVDAPWAASERERLGQIYVSALEEWARLYLDANQLDSCL
                     STCQLALEQNPYNELIYQFEMRVYAALGDRSSIVRCYQSCKSALEEGLGLPPSPEMEL
                     LYQELVNR"
     gene            2695..2874
                     /locus_tag="VFQ23_12510"
     CDS             2695..2874
                     /locus_tag="VFQ23_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907465.1"
                     /translation="MNNNFAQISVSGFSPTDGGHGPRGFSPTDGGHGPRGFSPTDGGH
                     GPRGFSPTDGGHGPR"
BASE COUNT          752 a          687 c          745 g          751 t
ORIGIN      
        1 tttatacgcg cagaatcacc atcaacatct ttccgacgag acagcccgag aatttgttga
       61 ccaaacaagc ggctggatta ctggcatggt gctttccaac atgccagata tgccgcgcgt
      121 ttccggtatt gataccttcg cctatctggg gcggcaggtg cttgatcaac aacctgccta
      181 tgtacgggaa ttcctaatgc gtacatcctt gtcagatgag ttcagtgccg atttctgcga
      241 gacgacgctg ggtccactgt acgctcaacg acaaaactgg tacgaactga tgagcctgat
      301 cctcgaaaag aatttattcg ttctgcctct tggctcggat gggcgttggc tgcgttacca
      361 tcacctcttc cgtgatttcc tgcaaacgcg gttgcgagag gaacgccctc atgaagtaac
      421 tcctatcctt gaacgaatgg tccaggcgta tgagaatgcg ggcgaatggg aaaaagccta
      481 tttcacatgc aaacaactga atgatttgga tgctctggca ggggtcgttg aaagagctgg
      541 cacacacatg ttgcaaaacg caaggattac cctggaaggt tggatcaaca gcctgcctcc
      601 gtccctgcta cggacgcgtc caggcttaat ctcattacgc ggaccaattc tggtggcgaa
      661 aggtaacttg caggaagcta ataatattct cgataaggca gtcactattt atcgaaagaa
      721 gcacgatatc cccgggctca cacttgcatt gatccgtcgt gccaatacgc tcagattcct
      781 ggggaattac agcgcatcca tccttgatac ggaggaggct ttgcaactgg ctgagtctga
      841 cacagcattt caatctctct acgccgaagc cttgcgcctc aaagggttga atctgcatcg
      901 gatgggtgaa tcacgtcatg cagttgagtc attggagcat tctctgtctt tgtacagcgc
      961 attaaatgaa acagacacga tcccgaccgt gttaatggaa acgggcatgg cccatcacgc
     1021 ggtcggcgat attgattcgg cccggagttc atatcaggaa gcactcaaat taaggcgggc
     1081 tgaaaaaaat gtgtattccc aggcagaaat actgaataat ttggcagtgc tctatcatca
     1141 actgggtgag tatgaactgg cctctgagac attcgaagac ggcttggtgt gtgcacgcaa
     1201 gagtcgcaac cagcgtaccg aatccctcat ccttgctggt ctgggtgatt tatatagtga
     1261 aatagaagaa ttcgacgcag cactgcaagc ctaccaacag gcaaaagcga ttgcgagcga
     1321 attggatgcc tcgtttatct cgaattattt gatcattgca aagggaatct tagccatcct
     1381 aagagaagat tggaaaagcg ccacccagat actgactaca cataaacgag gcataaaagt
     1441 aagtcagtcg gtgtatgaac ggggtctctg gtctttgttc gaaggtcggt attatcttct
     1501 gaaaggggaa gcgcgaaaag ccatccgtat cttgcaggag tgtaagaact tctttacgca
     1561 agatgggaga gatcttgaga ttcaatggag catgatctgg ctgggtgcag catttgagca
     1621 agccggccaa agaaacctgg ctcgcgcaga attcaaggag ctcttatctg tcatcgtcag
     1681 ccctgatcat gccttcctcg tcgcgtttcg cccggcggca gagtgggtaa tggatttgca
     1741 gaatgataca gaaattggac atcaggtgaa agccttgttg gagaagtcgc aacggctggc
     1801 gttgagactg ccttccatcc gtcgaatggt gcgtcgccat acccaactgg tccaaatgcc
     1861 atctgccagc atcattatcc gcacatttgg gcgtggtgaa gtaagtgtca acggtcagga
     1921 tattacaata tccgattggc ggacaaaatc tgtgagggat ctattcttct atttcttatt
     1981 taagcaggat tctcttacaa aagaacaaat cgccgaagtt ctttggcccg atgtgtccga
     2041 tcctgcgtca atcaagaaac gcttcaagga tgcgatttac tggatacggc gcgccgtcgg
     2101 aaaaaatgtg attgtttttg atcaggaata ttatcgtttg aaccgcacgc ttgactatga
     2161 gtatgatgtg gaggcctttg aatcatatct gaggcgcgca cgtaaaaaca aggatcgaac
     2221 tgaacggatt aactggtatc aaaaagcaac gaatctggtt cgtgggccct acctggctga
     2281 tgtggatgcc ccctgggctg cctctgagcg agaaagactg gggcaaatct acgtctctgc
     2341 gctggaggaa tgggctcgtc tgtatctgga tgcgaatcaa ttagacagtt gtctatccac
     2401 ttgtcagctt gcccttgaac aaaatcctta taacgaattg atctaccaat ttgagatgcg
     2461 tgtctatgcc gcccttggag atcgctcttc tattgtccgg tgctaccagt cgtgcaaatc
     2521 agctttggaa gaagggcttg gtttgccacc ttctccagaa atggagcttc tttatcaaga
     2581 attagtcaat cgctaaatcc attgtgatcg gggggaatcc tgcaaagttt tgcagcctgt
     2641 tttgcagcct gggggggagt atcatggaca catcaatcaa aaaagaggtg tttcatgaac
     2701 aacaatttcg ctcaaatttc tgtgtccggt ttttcaccca ctgatggcgg acatggccca
     2761 cgcggttttt cacctacaga tggcggacac ggtccacgcg gtttttcacc cactgatggc
     2821 gggcacggtc cacgcggttt ttcacctact gacggcggac atggtccgcg ttaatttagt
     2881 tataaaggag agttaactga ctatgctcac ttttgatgct taccctactt cagcg
//
LOCUS       DASTJP010000434         8407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1277923, whole genome shotgun sequence.
ACCESSION   DASTJP010000434 DASTJP010000000
VERSION     DASTJP010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277923"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFQ23_12515"
     CDS             complement(<1..658)
                     /locus_tag="VFQ23_12515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9907466.1"
                     /translation="MINRFRKIYHEFPQLFWIIVIARFIDALGGTILFPFFALYITQR
                     FGVGMAEAGVLLGMSSLFGLFGSMVGGALADRFGRRRLIVFGLVFSALSSLLFGLAND
                     FQVLYPLVIVVGLLSSVAHPAHDAMLADILPENKRQEGFGILRVVFNYAWIFGTAVGG
                     LIATRSFFALFMIDAVLSCIVAALIYRLLPETKPAAIDGENEEKSLWGTVVGYRLVLR
                     D"
     gene            complement(668..1477)
                     /locus_tag="VFQ23_12520"
     CDS             complement(668..1477)
                     /locus_tag="VFQ23_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907467.1"
                     /translation="MMKLKNLFFLILLIIPNFACNLSNQIPTAFPSAVTEHTATTILP
                     SETLTQTLTVASTFTLLPTDTALPSTPTEAPTSIPVPTVPSLRATVTADRLSCRYGPG
                     PEYLYLFALRQTANIKLIGRVDAENWDWFMVENQVNCWVKAEFLDIQGEPLSLPIVYP
                     NEAKLPVTPYYPPSEVLRAERDNITHEVTVSWVEVPVSLGDYEDDSMQTYIIEVWRCE
                     GGQLIFDPLATRFTAIKFMDEPGCAELSHGRVFVQEKHGYAGPAEIPWPLP"
     gene            complement(1672..2520)
                     /locus_tag="VFQ23_12525"
     CDS             complement(1672..2520)
                     /locus_tag="VFQ23_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907468.1"
                     /translation="MTIELVSAEQFTIQELTDLYNQTRVDYLVPMPMNASRLAEYVHD
                     YDIDLSKSCVARGEDGQTLGLCMLGVRPHQAWITRLGVLPATRRGGVGSALMDCMIEN
                     AVSTGVQKIQLEVIKNNTPAYQLFSCKGFEEASEYLVMRRAPRPSSEPPRGTVDWWNS
                     AAALDALKNYPNHLTWINDLASMQNAPDIEGLHLTLPNGSMGWLVYRNTKFTLRSTLS
                     HLVIHTINGNPVEVGTQLLLNLHTRYAHHDTYAENILKEDPHLLAFHALEYFENFSRI
                     EMFHSL"
     gene            complement(2517..2891)
                     /locus_tag="VFQ23_12530"
     CDS             complement(2517..2891)
                     /locus_tag="VFQ23_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9907469.1"
                     /translation="MAKILYIEDIQDNVTLVEKIITSRGHEFISAENAEMGLEMAFEG
                     HPDLILLDLGLPDADGQTLSVWMRNDPALEKIPIIVLTAWPEEVVRHTVRAYKLDGYL
                     CKPFTLAELVKIIDGTLSKKSS"
     gene            complement(3052..3435)
                     /locus_tag="VFQ23_12535"
     CDS             complement(3052..3435)
                     /locus_tag="VFQ23_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907470.1"
                     /translation="MKTISIFLALINSLLAGLLIIYSLSASEIRHVVAWWLVIKILAG
                     LAVIAAGALAWIGSVKPIRSSVMILSSLLLVALGAATLMWSLHLGLVTSEIEYYMFLY
                     GGSLIIQGTASLFGNSGTSENMTVA"
     gene            complement(3525..3962)
                     /locus_tag="VFQ23_12540"
     CDS             complement(3525..3962)
                     /locus_tag="VFQ23_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HET9907471.1"
                     /translation="MPLPPKLQEIVDDFSTMTREDKIETLIAFAESFPELPEWLRAER
                     DKMEPVPECMTPVRLFGEKQQNGGITFHFDIPPQSPTVRGLAAILANGLNGLKPEEIL
                     SVPADFFVPMKLEDAITHQRINGFIGVLAHMKHAALGLMDSKS"
     gene            complement(4007..4846)
                     /locus_tag="VFQ23_12545"
     CDS             complement(4007..4846)
                     /locus_tag="VFQ23_12545"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET9907472.1"
                     /translation="MSYVHPEYLVDTEWVAAHSDDPNIRIIESDEDPLLYAIGHIPNA
                     VQVDWFSTLQHPLRRDFLTKDKFEEEASKLGIGNDTTVVFYGDKSNWFACYALWIFQY
                     YGHQNVKIMNGGRLKWEQEGRPLVKDVPSFEKTDYKATDADKSIRAFRDEVLQQSESK
                     KPLVDVRSPKEYSGELISMPNYPQEGAQRGGHIPNAVSIPWAQAVNEADSTFKSADDL
                     RALYEGKGITPNGDVIAYCRIGERSSLTWFVLKYLLGYPNVKNYDGSWTEWGNLVDAP
                     IEK"
     gene            complement(5189..6271)
                     /locus_tag="VFQ23_12550"
     CDS             complement(5189..6271)
                     /locus_tag="VFQ23_12550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9907473.1"
                     /translation="MKEYDSSNPFLRVDMLTGCNNLVSFSKSLENNFDNTMYERTSLI
                     VVDLYQLREINQKKGFDHGDRLLRWLGIAIKDETGSVVYRISGANFIAVLIGGKHKTH
                     EATARKLFDRLNSEARQLEVDTPVARMAVIHFPDSAALNPPVVWRNINEKLELTSAET
                     PFQAFQADTSEEDTVTLHAIDLMAKRIETLGGTLQYTFKLAYTDPVSNAPNMLAIQRR
                     LDLALSEAILEQKPLSLCMIDGDNLKLYNSQGYQAGDEVIRKITNTLSGALRPSDFLG
                     RWRMGDEFIIILPSTSIEQASVVAERLCASIESASMGWLYPTTVSIGISYYPKHGETA
                     NKLLEVAEKGLRLAKMSGKNRVILAP"
     gene            complement(6369..6854)
                     /locus_tag="VFQ23_12555"
     CDS             complement(6369..6854)
                     /locus_tag="VFQ23_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HET9907474.1"
                     /translation="MYRFSSFGDFRCTKCGAFVTSAHQFSGVNNRNHCPYCLWSSHLD
                     LYTSGDRLSACKGPMKPIGLTMKKSRNKYRPESSGELMMIHECIECTAISINRIAADD
                     DTDSAFIVFRESLIMLHRLHRLCKPYEIVPLDHRDLETVETQLYGRNTIRSSLMWEAT
                     P"
     gene            complement(6854..7831)
                     /gene="rsgA"
                     /locus_tag="VFQ23_12560"
     CDS             complement(6854..7831)
                     /gene="rsgA"
                     /locus_tag="VFQ23_12560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET9907475.1"
                     /translation="MNTDNEDDIGIVFRKTTGHYTVHANGREIDCELSSLLHKQLIYP
                     TADPTSLRHKVQEVRELDHVDPVAIGDRVRFTDASEGRGMITEVLARRNKLSRPANQR
                     AFEQVIVANADLVVPVFAAASPTPKWGLLDRYLVSAESSDLPALIVINKIDLAWKNST
                     LDEDLDVYRQIGYPILSVSAVTGEGIEELQRFLQGKLSVLVGKSGVGKTSLLNAIQPG
                     LGLRVRAVSQGELGKGRHTTTHLEMFTLEFGGALVDTPGIRELGLWNIAREELAYLFP
                     EMADYVGKCKFGLSCHHDREPDCAIRTAVMSGDISPHRYKSYMQLREEL"
BASE COUNT         2133 a         2044 c         2108 g         2122 t
ORIGIN      
        1 gatcgcgcaa gaccaaacga tacccaacga cggttcccca aagagatttc tcttcgttct
       61 ccccgtcgat ggctgcaggc ttggtttcag gcaggagtcg atagatgagc gccgccacga
      121 tacaacttag aacggcatcg atcataaaca aagcgaagaa agaccgcgta gcgatcaagc
      181 caccgactgc ggtgccaaag atccacgcat aattaaacac gactcttaaa atgccgaagc
      241 cctcctgtcg tttgttttct ggcaggatat cggcaagcat ggcatcatgt gcagggtggg
      301 caacactgga gagaagcccc actacaatta ccaggggata taacacttgg aaatcattgg
      361 cgagtccaaa aaggagactg ctcaacgcac taaagaccag cccaaaaacg atcaggcgcc
      421 ggcgcccgaa tcgatctgcc aacgcaccac caaccatact tccaaataaa ccgaaaaggg
      481 atgacatgcc aagcagtacc cctgcctccg ccatgccaac tccaaatctc tgtgtaatgt
      541 aaagagcaaa aaatggaaaa agaatggtgc cacccagggc atcgataaaa cgtgcaatca
      601 cgatgatcca aaacaactgg ggaaattcgt gataaatctt tctaaagcgg ttgatcatga
      661 tgtggcttta tggcagcggc caggggattt ctgcgggacc agcgtagcca tgtttctcct
      721 gaacgaaaac tcgtccatga gatagttcgg cacaacccgg ctcatccata aacttaatcg
      781 ccgtgaacct tgtggcaagt gggtcgaaga tcagctgccc gccttcacaa cgccagactt
      841 caataatata ggtttgcatc gagtcatctt cataatcgcc caggctgacc ggaacctcca
      901 cccacgagac ggtaacttca tgagtgatat tgtcgcgttc tgcgcgcagt acttcagatg
      961 gcggataata tggagtgacg ggcagcttcg cttcattggg gtatacaatg ggaaggctta
     1021 gtggttcacc ctgaatatca agaaattcag ctttgaccca acagttgact tgattctcca
     1081 ccatgaacca atcccagttt tccgcatcga cccgaccaat gagtttaatg tttgcggtct
     1141 ggcgaagtgc aaagagatat agatattcgg gcccgggccc gtagcgacag ctcaatcgat
     1201 ctgctgtcac ggttgccctg aggctaggga ccgttgggac cggaatcgat gtaggggctt
     1261 cagttggtgt gctggggagt gccgtatccg ttggtaggag agtaaacgtg gaagcgacag
     1321 taagagtctg cgtcagcgtt tcagagggca gaatggttgt tgccgtatgt tcagttacag
     1381 cagatggaaa agcggttggg atttgattgc tgagattgca tgcgaaattt gggatgatca
     1441 ggagaataag gaaaaacaag ttcttgagtt tcatcatccc attgtactaa gtacctgccg
     1501 aaaaatcgtt gtaaatgagt tgctctcggc aggacgaatt tacgctcacc tttaagtagt
     1561 aatgcccagc ctgagggggg caggctgggc attactagac ataataatat cacagtcatt
     1621 caattttcgt caacgagatt tgataagaaa attattaaag ctttcaatga ttcaaagtga
     1681 atgaaacatt tcaatccgcg aaaagttttc gaaatactcc agagcgtgga aggcaagcag
     1741 gtgtggatcc tctttcagga tattttcagc atatgtatca tgatgtgcgt aacgggtgtg
     1801 caggttcaat aagagttgtg tgccaacctc aactggattc ccatttatcg tgtggatgac
     1861 aagatgacta agtgtggatc gaagcgtaaa ctttgtattg cgatagacca gccagcccat
     1921 gcttccgttt ggtaaggtca gatgcagacc ttcgatatcc ggcgcgtttt gcatcgatgc
     1981 caggtcatta atccaggtga gatggtttgg atagtttttc aaggcgtcca gcgcggcggc
     2041 agaattccac caatcgacgg ttccgcgcgg cggttcggaa gatggacgag gtgcacgtcg
     2101 catgaccaga tattcactgg cttcttcaaa tcctttacag gaaaacaact gataggcagg
     2161 tgtattattt ttgatcactt ccagttgaat cttttgtacc ccggtcgaaa ctgcgttttc
     2221 gatcatgcaa tccatcaagg cggagccaac gccgcctcgg cgcgtcgcag gcaaaactcc
     2281 aaggcgggtg atccatgcct gatgtggacg cactcccagc atgcacaggc cgagcgtctg
     2341 accatcttct ccgcgcgcga cacaagattt tgatagatca atgtcgtaat catgaacata
     2401 ttccgccagg cggctggcat tcattggcat gggaaccagg tagtccacac gggtctggtt
     2461 gtatagatct gtcaattcct ggattgtaaa ttgttcggcc gatacgagct caattgtcat
     2521 gatgattttt tacttaatgt accatcgata attttcacaa gctccgccag ggtgaacggt
     2581 ttacataaat acccatccaa tttatacgct cgtacggtat gacgtactac ttcctcgggc
     2641 caagcggtca gcacgataat agggatcttt tccagggcgg gatcatttct catccaaacg
     2701 gatagggttt gtccatcggc atctggcagc cccaaatcca gcaggatcag gtcgggatgg
     2761 ccttcgaatg ccatttcgag tcccatttcg gcattctctg ccgaaataaa ctcgtgccca
     2821 cgcgaagtga tgattttttc cacgagggta acattatctt ggatatcttc aatgtaaaga
     2881 atcttcgcca tgacctactc cttctttcta ctggctgcaa ccggcgagaa tttttacgag
     2941 gatctggcag attaacatga tatggctggg caattgtaat ccatccctcc atgaaaattt
     3001 ccgagccctg aattaaaaag aaagatcccg cttcggagcg ggatccgcgt cctacgctac
     3061 agtcatattt tcagacgttc ctgaatttcc aaagagcgag gcggtgccct gtatgatcag
     3121 gcttccgccg tacaagaaca tgtaatattc gatctcactc gttaccagac ccagatgcag
     3181 actccacatc agtgtggcgg cgcccagcgc tacaaggagc aaactactca aaatcatgac
     3241 acttgaacgg atcggcttaa cgctaccaat ccatgcaagc gctccggccg caatcacagc
     3301 cagcccggcc agtattttga ttactagcca ccatgcaact acatgacgta tctcactcgc
     3361 cgacaaacta taaataatta acagacctgc cagcaacgaa ttaatcaggg caagaaatat
     3421 ggaaattgtt ttcatcgcag tacctccttt tgggtataaa aaaaatacct ctgtgagact
     3481 atcaaagtct acacagaggc tatcaattcc cgtaagaagg tatcttaact tttgctatcc
     3541 attaagccga gcgccgcatg tttcatatgt gcgagcactc caatgaagcc attaatgcgc
     3601 tgatgtgtaa tggcatcctc cagtttcatc ggcacgaaaa agtctgcagg gacagaaagg
     3661 atctcttcgg gtttcaagcc gttcaatcca ttcgcaagga tggcggccag tccacgtacg
     3721 gtcggagatt ggggagggat atcaaagtga aatgtgattc cgccattttg ctgtttttca
     3781 ccaaaaagac ggacaggcgt catacattcg ggaacgggtt ccatcttgtc gcgttctgcc
     3841 cgaagccatt ctggcagctc tgggaatgac tcagcgaacg cgatcaaggt ttcgatcttg
     3901 tcttcgcgcg tcatggtgga aaagtcgtcc acgatttctt gaagtttggg tggtaaaggc
     3961 atattattac tgtaggggcg gacagctgtc cgcccctgca aaccatctat ttctctatcg
     4021 gtgcgtcaac taggttgccc cattccgtcc aggaaccatc atagttcttg acgttgggat
     4081 agcccaggag atatttcagc acgaaccagg tgagtgagga acgttcgccg atgcgacagt
     4141 aggcgattac atcaccgttt ggtgtgatcc ctttgccttc ataaagggcc cgcagatcat
     4201 cggcactctt gaaggtgcta tcggcttcat tgacagcctg tgcccaggga atgctgactg
     4261 cgttggggat atgcccgcca cgctgcgcac cttcctgcgg atagtttggc atagagatca
     4321 actcaccgga gtattccttg ggagagcgca cgtccaccag gggtttcttc gattcgctct
     4381 gctgtaagac ctcatcgcgg aaggcgcgga tggatttatc ggcatcggtg gctttgtaat
     4441 cggtcttttc aaaggatgga acgtctttca cgagaggtcg cccttcctgt tcccatttga
     4501 ggcgcccgcc gttcatgatc ttcacattct gatgtccgta atattggaaa atccataatg
     4561 cataacaagc gaaccagttg gatttgtcac cataaaacac gaccgtggta tcgttaccaa
     4621 tccccaattt cgaagcctct tcctcaaact tgtctttcgt gaggaaatcg cggcgtaatg
     4681 gatgctggag ggtgctgaac cagtctacct gcacagcgtt gggaatatga ccaatggcat
     4741 acagcaaagg atcttcatcc gattcgataa tgcggatgtt cgggtcgtcg gaatgtgcgg
     4801 cgacccattc cgtgtctacc agatactcag ggtgaacgta ggacatgttt gattttcctt
     4861 tttgaattgt gattttgcgt taagtggaac tcattaaaaa taatagcagc caacaaataa
     4921 atcaagggac agtgaacaat cctgcgcaga aggcatgtgg gtgcgcgggg gtcgttccac
     4981 catcctgagt tgttgactgc tgatcggata ccgatgtctc aggttgtggt tgcgagcccc
     5041 gctatagaca cgagcaatag ttaaggttaa tcatgggata tgaacaatga cgccgctatt
     5101 ttacaattct tacatgtttt gtcaagtcaa aaattatcag tgagaaggtt caaacgaccg
     5161 tccaactgcg tcaggaaccg cgtcggcttc aaggcgcaag gatcacccgg ttcttaccgc
     5221 tcatcttggc aagccgtaac cctttctctg ccacttcgag tagtttgttg gccgtctcgc
     5281 catgctttgg gtaataggaa atgccaatcg aaactgttgt ggggtacagc catcccatgg
     5341 atgctgattc aatcgacgca caaaggcgtt cggcaaccac actggcttgt tcaatactgg
     5401 tggaaggcaa aatgataata aattcatcac ccattcgcca ccgccccagg aagtcgctcg
     5461 gacggagcgc tccgctcagc gtattggtta tcttgcggat gacttcatct ccggcctggt
     5521 atccctggct gttgtaaagc ttgaggttat ctccatcgat catgcaaagg ctcagcggtt
     5581 tttgttcgag gatggcttcg ctcagtgcca ggtcgagccg gcgctggatc gcaagcatgt
     5641 ttggggcatt gctgaccggg tccgtataag cgagtttaaa tgtatattga agcgtgccac
     5701 ccagtgtttc aattctcttg gccatgaggt caatggcatg taatgtaact gtgtcttcct
     5761 cagatgtatc cgcctggaag gcctgaaagg gtgtttcggc actggtcagt tccagttttt
     5821 cgttgatgtt tctccacacc accggcggat tcagagctgc gctgtcggga aagtgaatga
     5881 ctgccattcg agcgaccggt gtgtccacct ccaactgcct ggcttcactg ttcaaccgat
     5941 caaacaattt tcgggcagtg gcttcgtggg ttttatgctt tcccccaatt aaaacggcta
     6001 taaagttcgc ccccgaaatt cgatagacaa cagaaccagt ttcgtccttg atggcgatgc
     6061 ccagccagcg aagtaaccga tcgccatggt cgaagccctt tttctggttg atttcgcgca
     6121 gttgatacag gtcaacaacg ataagcgaag tccgctcata cattgtgtta tcgaaattgt
     6181 tctcaagcga cttggaaaag ctgaccagat tgttgcaacc tgtcagcatg tcgacccgca
     6241 gaaaagggtt gcttgaatcg tattctttca tttgactccc agtagtgtaa gcgacgacat
     6301 tttctattat agggcaaaaa acctcacgta aacgtgaggt tttgttacag taaccgattt
     6361 gttattattt agggcgtcgc ctcccacatg agtgaagatc ggattgtgtt gcgcccatat
     6421 agctgtgttt ccacggtttc caggtcacga tggtcgagtg ggacgatctc gtacggcttg
     6481 cacaagcgat ggagcctatg gagcatgatc agagattctc gaaacacgat aaacgctgaa
     6541 tctgtatcat catctgccgc gatccgatta atggaaatcg ccgtgcactc aatgcattca
     6601 tggatcatca tcaactcgcc gcttgactcc gggcggtact tgtttctgct ctttttcatg
     6661 gtcaacccga tcggcttcat cggccctttg caggcggaca aacgatcacc cgaggtatac
     6721 agatccaggt gagacgacca taagcaatat ggacagtggt tgcggttgtt tacgcctgag
     6781 aattggtgtg cggaggttac aaatgccccg cattttgtac aacggaaatc gccgaaggag
     6841 gaaaagcggt acattagagc tcctcccgca gttgcatata acttttatag cgatgcggac
     6901 tgatatcccc agacatcact gccgttcgaa tggcacaatc tggttcgcgg tcatggtggc
     6961 aactcaagcc aaacttacat ttgccgacat aatctgccat ctcggggaaa aggtaagcca
     7021 gttcttcacg ggcaatattc cacagaccta attcgcgtat gccgggcgtg tcgactaacg
     7081 cgccgccgaa ctccagcgtg aacatctcga gatgagtcgt agtgtgccgc cctttgccca
     7141 gctcaccctg gctgacggcc cgaacacgca ggccgagccc cggctggatg gcattgagta
     7201 gggatgtttt ccccacgccg gatttcccga ccagcacaga tagcttgccc tgcagaaaac
     7261 gttgtagctc ttcgattcct tcaccggtca ccgcgctgac agaaaggatg gggtaaccaa
     7321 tctgtcgata gacatccaaa tcctcatcca gagtggaatt cttccaggcc aggtcaattt
     7381 tgttgatcac gatcaaagct ggcaggtcag aggattctgc ggagacgaga taacggtcga
     7441 gcaatcccca ctttggggtg ggactggctg ccgcaaacac gggcacgacc aggtccgcgt
     7501 ttgccacgat gacttgttcg aaggcacgtt ggttcgcggg ccgtgaaagt ttgttccgcc
     7561 ttgccaggac ctcggtgatc atgccgcggc cctcacttgc atccgtaaag cggacgcggt
     7621 caccaatcgc tacggggtcg acatgatcca gctctcgcac ttcctgcact ttgtgacgca
     7681 gggaagtcgg gtcggctgta gggtaaatca gttgtttatg taataaggac gaaagttcac
     7741 aatcgatctc tcggccgttt gcgtggacag tgtaatgtcc ggttgttttt cgaaagacga
     7801 tacctatatc gtcttcgttg tctgtattca ttgtcagggt ttcctgtaaa tgcgctccat
     7861 ttggatcgaa tgaggatcgc gatgtgcaca taagtttgat tgaaaggaat ttgcgatagc
     7921 tgcttgcatt gaactgcgcg agcaggaatg tgttgagggt attgattaac ttgagcgact
     7981 tgtgctgaca taaaattctt gctcctttct gaaaataaaa aaccggctac ggagattccg
     8041 tggccggtaa aaattttgca gcaggcgtct agggctgctt cccagggtag gtgtgcagtg
     8101 caaggtgata gacgcggctg ttaaataaaa acgccacgga caagggtacg tctgtggcaa
     8161 ttccgtttag gtaaatcgaa aaactggctt aactgctatg cagagacgcg cccatgggtt
     8221 tcttcgctaa caagcaccga tgatattttg aacataaaac gcgtctcctt tctttgagat
     8281 aaagttcaac gatacgaagt ttaccaacag ccaaaaaggc tgtcaaggta aattcagtaa
     8341 atcaccatgg ttttctcaag aaccagaata ggtggttttt gtacacggat ttcacggaag
     8401 ggacgga
//
LOCUS       DASTJP010000435         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_322128, whole genome shotgun sequence.
ACCESSION   DASTJP010000435 DASTJP010000000
VERSION     DASTJP010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322128"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="VFQ23_12565"
     CDS             <1..1773
                     /locus_tag="VFQ23_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9907476.1"
                     /translation="IPMAHLYLVTKPVKDQPPSMPTMRDPDNLVYFRGTGSGMIAGGY
                     ERQPAPWGLNGVPQDWNFKLLEPDWPRFTPLMESAIKRVPSLETAEIVQLLNGPEGFT
                     PDSEYLLGPTAVRGFWLACAFCAHGIAGAGGIGKVIAEWIIDGHPEWDMWKLDARRFG
                     NQYASQSFTLARTIETYGKYYDIHMPYEERESARPLRTSPAYHRLKDLGAVFGEKGGW
                     ERPNWFTRNEEDARATSLPDLSGWSLDNWSPAIAAETRATREAAGLFDETSFSKIEVI
                     GPGACEFLQKLCGNDIDKPVGSVTYTQMLNARGGIECDFTVSRLANDRFMIVTGTAFG
                     THDMTHLRRYMPLDGSVYINDLTSARTCFGLWGPRARDILQKVTRDDVSNSAFPYMTA
                     KQITIGNIPALALRVTYVGELGWEFYAPSEYGEQLWDTLWEAGKEFGMVAAGYKAIES
                     LRLEKGYRYWSADITPDYTPYEAGLGFAVSLNKNSDFIGKQRLQGQKAEGVRLKLCCL
                     TLDDPNTIVFGSEPIRYEDNVVGWVTSGGFGYSINKSIAYAYLPVELAKAGTRLSVEC
                     FGAQIPAEVQKEPLFDPKGEKIKA"
     gene            1770..>2025
                     /locus_tag="VFQ23_12570"
     CDS             1770..>2025
                     /locus_tag="VFQ23_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907477.1"
                     /translation="MMSAHIKAIIFDYGNVLIEWNPRLVYRRFFPNDEAGMESFLSEV
                     QFMEWNAQQDKGRTFEEGVTILSEQFPQYAELIRAYHENWV"
BASE COUNT          483 a          504 c          559 g          479 t
ORIGIN      
        1 atccccatgg ctcacttata tctggttaca aagcccgtga aagatcagcc accatccatg
       61 ccgaccatgc gcgacccgga taacctggtc tatttccgcg gcaccggcag cgggatgatc
      121 gcgggtggct atgagcgtca acctgcaccc tggggcttga atggtgtacc gcaggattgg
      181 aacttcaaat tgcttgagcc cgattggccg cgcttcacac ctttgatgga aagcgcgatc
      241 aaacgcgtcc cgtctctcga aacggcggag atcgtccaac tgctcaacgg acccgaaggc
      301 ttcacgcctg attcagaata tctgctaggt cccaccgccg tgcgcggctt ttggctggcg
      361 tgtgcatttt gtgcgcatgg catcgcgggc gcgggcggca tcggtaaggt aattgccgag
      421 tggatcattg atggtcatcc cgaatgggat atgtggaagc tcgacgcgcg ccgctttggc
      481 aatcaatatg cgagtcagtc gtttacgttg gcacgcacga ttgaaaccta cgggaaatat
      541 tacgacatcc acatgcccta tgaagagcgc gagtcagcgc gtcccttgcg gacctcccct
      601 gcttatcatc gcctgaagga tttgggcgcg gtcttcggcg aaaaaggtgg ctgggaacga
      661 ccgaattggt tcacgcgaaa tgaagaggat gcacgagcca cctccctacc ggacctctcg
      721 ggctggtcgc tcgacaattg gtctcctgcc atcgctgcag agactcgtgc cacgcgcgaa
      781 gccgccggac tcttcgatga gacgtcattt tcgaagatcg aagtgattgg tcccggtgcg
      841 tgcgaattct tgcaaaagct atgtggcaac gacattgata aacctgttgg ctcagtgaca
      901 tacacccaaa tgttgaatgc acgtggaggt atcgaatgtg acttcacagt ctcgcggctt
      961 gccaatgatc ggtttatgat cgtcacaggg acagcctttg gcactcacga tatgacgcat
     1021 ctgcgccgtt acatgcctct ggatggatcc gtttatatca acgatctcac ttccgcacgg
     1081 acttgttttg ggttatgggg tccgcgcgcc cgcgacattc tgcaaaaagt cacgcgcgat
     1141 gatgtttcca attcagcgtt tccatatatg acggccaaac agatcacgat tgggaatatt
     1201 cctgctctgg ctttgcgcgt tacgtacgtc ggtgaacttg gctgggagtt ttacgcgccc
     1261 agtgagtacg gcgaacaatt atgggatacg ttgtgggaag ccggcaagga gtttggcatg
     1321 gtcgccgcag ggtacaaggc cattgagtca ctgcgccttg aaaaaggcta tcgctattgg
     1381 agcgcggata tcacacctga ctatactccg tacgaagcag gtttggggtt tgctgtttcc
     1441 ctgaataaga attcagattt cattggcaag caaagacttc aaggacaaaa ggcagaaggt
     1501 gtaagactaa aattatgttg tctcacgctg gacgatccaa acacaatcgt ttttggaagc
     1561 gaaccgattc gctatgaaga caatgttgtt ggctgggtca catcgggtgg attcggttac
     1621 tcgatcaata aaagtatcgc ttacgcgtat ctccccgtcg aactcgcaaa agcaggaacg
     1681 cgcctgagcg tggaatgttt tggggcgcag attccggcgg aggtccagaa agagccgttg
     1741 ttcgatccca agggcgaaaa gatcaaagca tgatgtcagc acatataaaa gcaatcattt
     1801 tcgattatgg caacgtcctg atcgagtgga atccaagact tgtttatcgt cgcttctttc
     1861 cgaatgacga ggcaggaatg gaaagctttc tgagcgaagt ccaattcatg gaatggaatg
     1921 cgcagcagga taaaggtcgc acttttgaag agggcgtaac aattctttcg gagcaatttc
     1981 cacaatacgc cgagctaatt cgggcttacc acgagaattg ggtcg
//
LOCUS       DASTJP010000436         6350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_992956, whole genome shotgun sequence.
ACCESSION   DASTJP010000436 DASTJP010000000
VERSION     DASTJP010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992956"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VFQ23_12575"
     CDS             complement(<1..1196)
                     /locus_tag="VFQ23_12575"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9907478.1"
                     /translation="MSSQTTNKFQVPLWRQLVVYGLVILVVSTLTSRLISLQVMGEQT
                     WNAQAVDNYTNTISDPAPRGIIYDRNGYILARNVASYNVVITPAGLPDDDADIQHIYR
                     ELSELIDVPAGGPVTEASLEQAKLFAACVPGPPIADMVALGDSLAPYSPVRIKCNIGE
                     QLARIVSERSVDWPGVSVEIEPIRDYPTGSLTANVVGFLGPIPASQEEAYSNRGFIPN
                     RDKIGYSGVETSLDDVLIGRNGSRVVQIDVAGQVLRNIEAPIPSVAGNNVVLTIDTRL
                     QKAAEAALVDEINYWNTFFGNIRISSGIIIAMNPKTGEILAMVSYPTFENNRMARFIP
                     GYYYEQLIQDPRRPLVNNAISGIFPPGSTFKLSTATGAFNEDVVGPDQIIDTPGRLVL
                     CERFSP"
     gene            complement(1193..1693)
                     /locus_tag="VFQ23_12580"
     CDS             complement(1193..1693)
                     /locus_tag="VFQ23_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907479.1"
                     /translation="MRNVVALPVIVLAVILQTAIVSRVYLLSGIADLPLVMLAAWALQ
                     EEVETAWHWAVATGLLVGFVSALPMLAPLIGYFAVVGLAQILQGRVWQAPLLAMFSVT
                     FLGSVIASLFSFAVLSLSGIPLPVGDVLGVLTLPSVLLNMLLAIPVYAVMRDLARWVY
                     PTPEVE"
     gene            complement(1698..2537)
                     /gene="mreC"
                     /locus_tag="VFQ23_12585"
     CDS             complement(1698..2537)
                     /gene="mreC"
                     /locus_tag="VFQ23_12585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET9907480.1"
                     /translation="MRNLFSRTLQSTIIFLVVGGVMALALGGYFSSASNVFTGSLIDL
                     QAWFSSRFVAVQDFITAPRDMASLRQRNIELEADNAELQAQVIQLQQQVGETEVLAAL
                     VDFSRARPENTYRAAAVIGRDPSPFLHYIIINRGSNDGILRGMPVVTDQGLVGRIDAV
                     IADAARVQLITDPASTVNVRLQNAEVDASLTGSVTGDVELDLIPQDTLIEIGDLALTS
                     GLGGGYPPDLIVGQVINVRSRDFDLFQQATVQPVVDFNHLSIVLVIVNFQPVDIAPLV
                     PLP"
     gene            complement(2700..3770)
                     /locus_tag="VFQ23_12590"
     CDS             complement(2700..3770)
                     /locus_tag="VFQ23_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9907481.1"
                     /translation="MAFNPINWLLGLFSLDIAIDLGTANTLVNVRGKGIVINEPSWVT
                     IDKRLRQPLAIGLEAKAMMGRTPGNVAVIRPLRDGVISEFDITQVMLEYFIGRVHEQS
                     VVPLPRPRVVVGIPTGVTEVEKRAVYDAVMASGARAAYLIEEPIAAALGAGLPVADVR
                     GSMVVDIGGGTTEVAVMSMSGVVASRSLRVAGDEMDQDIVQYLRNKYNLLVGEGIAEQ
                     IKWRIGSAYSLQPEKTMEVRGRNLVTGLPESIEVSSIEMREALAGSVQVIVDTIRDAL
                     DEIPPEIVADLMDIGICLAGGGGLLQGLADRLTDELRLRVWVAEDPLTCVARGAGMVF
                     ENFDVLSRYLVGLERGSTRHTM"
     gene            3908..4762
                     /locus_tag="VFQ23_12595"
     CDS             3908..4762
                     /locus_tag="VFQ23_12595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET9907482.1"
                     /translation="MSQIAVVTDSTTYLPPELVKKHNISIAPQVLIWGDKTYKDGVEI
                     QSEEFFTKLKTAKVMPTTSQVAVVSFQEIFQGLVDKGFEVLAILVSSKLSGTIQSAAQ
                     AKELMGSAGEKVHIVDSQSVAMALGFQALAVARALESGASLKEAIALGEKSHEYTGVY
                     FAVDTLEFLHRGGRIGGAQRFLGTMLNMKPILAIHDGKVEGIERIRTKSKAHDRVLEL
                     VLEKTKGKTPVRLATLHANATEDAKALLARAEKQLSPIESIFTEVSPTVGTHAGPGTV
                     GLAFMAGM"
     gene            4835..5458
                     /gene="udk"
                     /locus_tag="VFQ23_12600"
     CDS             4835..5458
                     /gene="udk"
                     /locus_tag="VFQ23_12600"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:TIGR00235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET9907483.1"
                     /translation="MKSPDPLVIGIAGGSGSGKTTVVQEILQRVGRERIAFLQHDSYY
                     KNLTGLPPTQRASVNFDHPNSLETELLIQHVASLRDGKPVEVPIYDFSTDSRTGQTFT
                     VEPRRVILVEGILIFTEAALREMFDVKIFVDTDSDLRFIRRLQRDINERGRTTESVVE
                     QYLSTVRPMHLEFVEPSKRYADVIIPEGGFNTAALDMVVARVNALLK"
     gene            5500..6012
                     /locus_tag="VFQ23_12605"
     CDS             5500..6012
                     /locus_tag="VFQ23_12605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9907484.1"
                     /translation="MAKQWSTPPAMQIDAKKKYKAHMETDKGTMVIELFADKAPKTVN
                     NFVFLSREGYYEGVMFHRVISNFMAQGGDPTGRGSGGPGYKFEDEFHPSLRHDKQGIL
                     SMANAGPGTNGSQFFITHGPTPHLDNRHTVFGQVVEGLDVLMSIPPRDPGNVNAPAVK
                     INRVTIEETE"
     gene            5987..>6350
                     /locus_tag="VFQ23_12610"
     CDS             5987..>6350
                     /locus_tag="VFQ23_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907485.1"
                     /translation="MSPSKKPNKRPASKRSNSAQKENPKSSAPADSRSSSQTSSVAQG
                     THSQNQKAPRKTNLRTTILRVLAILAVISITLYTYTLRDRIAEFQALGYPGIFLVALI
                     ANATILLPAPGAALVAAVG"
BASE COUNT         1706 a         1711 c         1537 g         1396 t
ORIGIN      
        1 ttcggagaaa atcgctcaca gagcaccaac ctgcctggcg tatctatgat ttgatctggg
       61 ccgaccacgt cttcgttaaa ggctcctgta gcagtcgaaa gcttaaaggt cgagccagga
      121 gggaaaattc cggaaatcgc attgttgacc aaaggacgtc ggggatcctg aattaattgc
      181 tcatagtagt aaccgggaat gaaacgcgcc atccgattat tctcgaaggt tgggtaagaa
      241 accattgcca aaatctcacc cgtcttggga ttcatggcaa tgataattcc gcttgaaatt
      301 cggatattgc caaagaacgt gttccagtag ttaatctcat ccaccagcgc ggcttcggcg
      361 gccttttgca gccgtgtatc aattgtcagt acaacgttat ttcccgctac agaagggatc
      421 ggagcctcaa tattccgcag cacctggcct gccacgtcga tttgaacgac tcgcgatccg
      481 ttccttccga tcagaacatc atccagggag gtctcgacgc cggaataccc gatcttgtcg
      541 cggttgggga taaagccgcg gttgctatat gcctcttcct gagaggcagg aatcggtcct
      601 aaaaagccga ccacgttggc agtcagcgag ccggttgggt aatcgcgaat cggttcgatt
      661 tctacagaga ctccgggcca atctaccgag cgttcactga caatgcgagc cagctgttca
      721 ccgatattgc actttatacg cactgggcta tacggtgcaa gcgagtcgcc aagcgcaacc
      781 atatctgcaa tcggcggacc aggtacacac gccgcaaaga gcttcgcctg ctccagcgag
      841 gcttcggtta ctggtccacc cgccggaaca tcgatcaatt cggataattc gcgatagata
      901 tgctggatat ccgcgtcatc atcgggcaaa cctgccggtg tgatgacgac attataagag
      961 gccacatttc gagcaaggat atagccattg cgatcataaa taatgccgcg tggtgcgggg
     1021 tcactgatcg tatttgtgta attgtcaacc gcctgagcat tccaggtttg ctcacccatc
     1081 acttgcaatg agatcaatcg ggatgtaagt gtactcacga ccaatatcac caacccgtat
     1141 acaaccaatt gacgccaaag cggaacctga aacttatttg ttgtttgcga actcactcaa
     1201 cctccggagt gggatatacc catcttgcca aatctcgcat caccgcatag accgggatag
     1261 cgagcagcat attaagcaga acgcttggca aagtaagcac acccaaaaca tcacctactg
     1321 gtaaagggat accagaaagg ctcaaaacag caaaggaaaa taggctggca atgaccgatc
     1381 caagaaacgt aacactgaac atcgccagca agggggcctg ccacactcga ccctgcaaga
     1441 tctgtgcaag tcccactact gcaaaataac cgataagcgg cgctagcatg ggcaatgccg
     1501 aaacaaaccc cacgagtaaa cctgtcgcta ccgcccagtg ccatgcagtt tcaacttcct
     1561 cctgtaaagc ccaggcggca agcatcacta acggtaaatc tgcaatccct gaaagcaaat
     1621 agacccggct cacaattgct gtttgtagaa taacagccag gacaattaca ggtagtgcaa
     1681 cgacatttcg cattcgatta cggaagagga actagcggag caatatccac tggttgaaaa
     1741 ttgacaatca ccagaacaat ggaaaggtga ttaaaatcca cgacgggttg gactgtcgcc
     1801 tgttggaaga gatcaaaatc acgcgagcgc acatttatca cctgcccgac aatcaaatca
     1861 ggagggtaac ctcccccgag accagaggtc agcgcgagat ccccgatttc aatcaaggta
     1921 tcttgcggaa tcaaatccaa ctctacatca ccggttacag agccagtaag cgatgcatcc
     1981 acttcagcat tttgaagacg aacattcacc gtagaggcgg ggtctgtgat caactgaacg
     2041 cgagcagcgt cggcgatcac cgcgtcgatg cggcccacca gtccctgatc cgttacaacg
     2101 ggcatgccgc gcaagatccc gtcatttgag ccgcgattga tgatgatata gtgtaggaat
     2161 gggctgggat cgcgcccgat cacagccgca gcacgatagg tgttttcggg acgtgcccgc
     2221 gaaaaatcga cgagggccgc aagcacttct gtctcaccga cctgctgctg taattgaatc
     2281 acttgcgcct gcaattcggc attatccgct tccaactcta tgtttcgctg gcgcaacgag
     2341 gccatatcgc ggggcgcggt gataaagtcc tgcacggcca caaagcgtga cgaaaaccag
     2401 gcctgcaaat cgatcaagga tccagtgaat acattggatg cactgctgaa gtaccctccc
     2461 agtgccagcg ccatcactcc ccccacgacc aaaaaaatga tcgttgattg tagcgtacgg
     2521 gaaaataaat ttctcatatt acaaagtgcc gcattaccat ccaacccaga ttttgaacga
     2581 aacgtagaat tgtttcaaca aaacctggta ataagtgaaa caagcggtag tgtgctgctg
     2641 acctgattac tcaaaccgcc gccatccgcg aattgcaggt caccgagagc aaataaccat
     2701 cacatggtgt gacgtgtact accgcgttcc aaaccaacca gataccgact caggacatca
     2761 aaattttcaa agaccatgcc agcaccacgc gcaacacagg tcagcggatc ttccgcaacc
     2821 cacacgcgga gtcgaagttc atcggtcaaa cgatcagcca ggccctgcaa taaaccacca
     2881 ccgcctgcca ggcagatacc gatgtccatt agatctgcca caatctcagg tgggatctca
     2941 tccaacgcat cacggatcgt atccacgatc acctggacgg aacccgctaa ggcctcgcgc
     3001 atttcaatgg aagagacctc aatactttca ggaagtccgg tgacaagatt gcggccgcgg
     3061 acctccatcg ttttttccgg ctggagcgaa taggccgatc caattctcca tttgatttgt
     3121 tccgctatcc cctccccaac caggagatta tatttgtttc gcaaatattg cacgatatcc
     3181 tggtccatct cgtcgcctgc gacccgtaac gaacgcgagg cgaccacgcc actcatagac
     3241 ataactgcga cttccgtggt gccgccgccg atatccacga ccatacttcc gcgcacgtca
     3301 gcaaccggta gaccggctcc cagggccgcc gcaatgggct cctcgatcaa atatgcagca
     3361 cgcgcgccag aagccatgac tgcatcataa accgcgcgct tctcaacttc ggtgacacca
     3421 gttgggattc ccaccacgac ccgcggtcgc ggaagcggga cgacactttg ttcgtgaact
     3481 cggccgataa aatattccag cattacctgc gtaatatcaa attcagagat cacaccatcc
     3541 cgtaacgggc gtatcacagc cacatttccg ggtgtacgac ccatcatagc tttggcttcc
     3601 aggccgattg ccagaggctg tcgtaatcgc ttgtcgatgg tcacccagga aggttcgtta
     3661 ataacgatcc ctttgccgcg cacattcacc aaggtattgg cggtccccag atcaattgca
     3721 atatcaagtg aaaacaggcc taacagccag ttaatcggat taaacgccaa attcggctcc
     3781 tcgatgacgg tctcaaagca ataggtttgc taagttatta tacctaacaa ttcgtatcct
     3841 cgtgcctgat aagcgtgtta aaataccaag gattcgagcg taaaatctga aaatttatgg
     3901 aggagccatg tcacagatcg cggtagttac cgatagcact acttatcttc cccccgagct
     3961 cgtcaaaaag cacaatattt ccatagctcc acaggtacta atttggggtg acaagacgta
     4021 taaagatggc gtagagatcc agtctgaaga attctttacc aaactcaaga ctgcgaaagt
     4081 gatgcctact acttctcagg ttgccgtggt ttcctttcag gaaattttcc agggtctggt
     4141 ggacaaggga tttgaagtgc tggcgatcct ggtttcctcc aagctatcgg gaacgatcca
     4201 atctgccgct caggcgaaag aacttatggg gtcagccgga gaaaaggtcc atatcgtgga
     4261 tagtcagtcc gttgcgatgg cacttggctt tcaagcgctg gctgtggctc gcgcccttga
     4321 gagtggggca agtctcaagg aagcgatcgc actcggagaa aaatctcatg aatataccgg
     4381 ggtttacttc gcagtagata cattggaatt cctgcatcgc ggtggacgta tcggcggcgc
     4441 gcaacgattt ttgggcacca tgttgaacat gaaaccaatc cttgccattc atgatggaaa
     4501 ggtggaaggc atcgaacgca tccgcacaaa aagtaaggca catgaccgcg tgcttgagct
     4561 ggtgctcgaa aagacaaaag gaaaaacccc ggttcggctt gcaacattac atgccaatgc
     4621 gacagaagat gcaaaggcat tgctggcgag agcagaaaag caactcagcc ccattgagag
     4681 tattttcact gaagtaagtc cgactgtcgg aactcatgcg ggccctggta cggtcgggct
     4741 tgcattcatg gcgggaatgt aaacttttca ctactacttt ccctctcccc gcttggcagg
     4801 gagagggatt tgtttatccg tgtataattc aaatatgaag agtcctgacc ctctggtgat
     4861 cggcatcgca ggcggatcgg gctctggtaa gacaacggtc gttcaggaaa ttctgcaacg
     4921 ggtcggacgc gagagaatcg catttcttca gcacgactct tactataaga acctgaccgg
     4981 cttaccgccc acgcaacgcg catccgtcaa cttcgatcat cccaattcac tagaaactga
     5041 actactgata cagcatgtcg ctagcctgcg cgacgggaag ccggtggagg tgccaatcta
     5101 cgatttctcg accgacagcc gcacgggaca aaccttcacg gtagaaccac gccgggtaat
     5161 tttggtagaa gggattttga tctttaccga agctgctttg agagaaatgt tcgacgtgaa
     5221 aatatttgtg gacacggatt ctgatcttcg cttcatccgc cggctccagc gcgacatcaa
     5281 cgaacgtggg cgaacaacgg aatctgtggt cgagcaatat ctatccacgg tgcgccccat
     5341 gcatttggaa tttgtcgaac cctcgaagcg ctatgctgat gtgattattc ccgaaggtgg
     5401 tttcaatacc gcggcgctcg atatggttgt cgcaagagtg aacgcattgt taaagtagag
     5461 cacacaaaat ttgaacggat tctaaaaagg agatattaaa tggcaaaaca atggtcaacc
     5521 ccacctgcaa tgcagattga cgcaaagaaa aaatacaaag ctcacatgga aacagataag
     5581 ggcacaatgg tcatcgagct atttgctgat aaagcaccaa aaactgtgaa taactttgtt
     5641 tttctttcgc gagaaggata ttatgaaggc gtgatgtttc atcgcgtgat ctccaatttt
     5701 atggcgcagg gcggcgaccc aaccgggcgc ggctctggag gtccaggtta taaatttgaa
     5761 gatgaatttc atcccagcct tcggcatgac aagcagggca tcctctccat ggcgaatgcc
     5821 ggcccgggca ccaatggctc tcaatttttc attacgcatg gtcctacacc ccacttggat
     5881 aacaggcaca ctgtatttgg tcaggtcgtg gagggactgg atgtgctcat gtcgattccg
     5941 ccgcgcgatc ccggtaacgt caatgcaccg gctgtgaaga tcaaccgtgt caccatcgaa
     6001 gaaaccgaat aaaaggcccg cttcaaaacg aagcaactct gcgcagaaag aaaatccaaa
     6061 gtcgtctgcg cccgctgatt ctcgtagtag ctcccaaacc tcgtcggttg ctcagggcac
     6121 tcattctcaa aatcaaaagg ctcctcggaa gacaaacctt cgcacaacaa tcctgcgcgt
     6181 tcttgccatc ctggccgtaa tttcgattac gctatacacg tacacccttc gcgaccgcat
     6241 cgccgaattc caagcgctcg ggtatcctgg aatcttcctg gtggctctta tcgctaatgc
     6301 gacgatcctg ctccccgcgc cgggtgccgc actggtggcc gcggtgggtg
//
LOCUS       DASTJP010000437         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_761415, whole genome shotgun sequence.
ACCESSION   DASTJP010000437 DASTJP010000000
VERSION     DASTJP010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761415"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFQ23_12615"
     CDS             complement(<1..258)
                     /locus_tag="VFQ23_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907486.1"
                     /translation="MFAGLEVGLGLIIIATVLFLSVLFVVLLRRLPRNSLLAPDSVTS
                     FVSTDTSNSNESILIIQPGGRVEYLNDVARELFGLRSDEPTD"
     gene            complement(273..1172)
                     /locus_tag="VFQ23_12620"
     CDS             complement(273..1172)
                     /locus_tag="VFQ23_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9907487.1"
                     /translation="MGKARLLVVEDDVDIGNMLKIYFSGMEFDVDVAVRGSDALEKTK
                     QSLPHLIVLDIMLPDIDGYEVCRNLRTNLRTSHIPVIFLTQKDERSDKLQGLELGADD
                     YITKPFDIEELKLRVQGAIKRSERESLTDPRSGLPAGRLIENRLREIIREKGWALLDA
                     GINHFDAFKNVYGFVAGDDVLRFSAIMIGEIVDEVGNPTDYIGHAGGDNFIIITTEEK
                     AEIMKSRLKERFDQEVQTHYNFIDRQQGFVQTPMSDGTTVKAPFMTMTIGVVSPSQHS
                     FADIREITELSAEARRQDSAAGG"
     gene            complement(1188..2288)
                     /locus_tag="VFQ23_12625"
     CDS             complement(1188..2288)
                     /locus_tag="VFQ23_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907488.1"
                     /translation="MDTFAFIIHPIDPKRDVSRKFPLLGRFLNERQIDFFSTFFPPVY
                     ISEIEGIKSEATGKEIKGWFIACPYTPRRMMELPEQTVYRKIIETGRLAEKLGAQILG
                     LGAFTSVVGDAGITIANALDVPVTTGDSYTVTVAVDALREAAYVMDIPLQGTTAAVVG
                     ATGAIGQVCADLLADDVERLYLIGRRQDALEELRDRLKVHARAELILSTTMDVLSDAQ
                     LILTVTSSIHDVIRPEHLQPGSVVCDVARPRDVSAMVAAARDDILVIDGGMVDVPGPV
                     DFHFNFGFPAGKAYACMAETIALALEGRFEDYTLGKHLTRARVEEIDSIAKKHGFRLS
                     GFRSFEREVTEAQIETVRRNAKLARKLHAAQT"
     gene            complement(2292..>3278)
                     /locus_tag="VFQ23_12630"
     CDS             complement(2292..>3278)
                     /locus_tag="VFQ23_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HET9907489.1"
                     /translation="AFKEHALPLSNSYAPAGEHYLADLWYYPRGPVADVEPLVINLEA
                     REIGYYHVPTYMADQSGDLVFQVPLRALIAIDSWVHIFIVDVVFGLFSRGARFEKRLG
                     EDLGFKLKILGKAIYEGRQILECSELVKVGKNCVIDPHAIIHGPTTIGDNVTINAGAV
                     IENCIIGNNVNISQDVQLMLCVVGDGAFLPFRAALFMTTVMENSMVAQNTCLQMCVIG
                     RNTFIGAGSTFTDYNLIPAPIRALDGQGKLNISNRPVIGSAVGHNCRLGSGMIVYPAR
                     TIESDVVLAASKDRRVIDKDIRYEDSDHHKLKSASLHRRLYPRPGEGELESW"
BASE COUNT          870 a          882 c          742 g          784 t
ORIGIN      
        1 atctgtcggc tcatctgaac gtaagccgaa cagctcgcgc gcaacatcat taagatattc
       61 cacccgccca cccggctgga tgattaagat cgactcattt gaatttgaag tatcagtgga
      121 aacaaacgat gtaacagaat caggtgctaa aagtgaatta cgcggcaggc gtcgcaggag
      181 gacaacgaac agtactgaaa gaaacagcac tgttgctatg atgatcaacc cgaggcccac
      241 ttccagcccg gcaaacatta tccgatatca ccttatccgc cagccgcaga atcctggcgg
      301 cgcgcttcgg cagacaattc ggtaatctcg cgaatatcag caaaagaatg ttgactcggc
      361 gaaaccacgc cgatggtcat ggtcataaac ggcgctttca ctgtagtgcc atcagacatg
      421 ggtgtttgga caaatccctg ctggcgatcg ataaagttat agtgcgtttg cacttcctga
      481 tcaaacctct ctttgaggcg cgacttcatt atttctgcct tttcctcggt cgtgatgatg
      541 atgaaattat caccacccgc gtgaccgatg taatccgtcg gatttccaac ttcatccacg
      601 atttcaccaa tcatgatggc agaaaagcgc aaaacatcat caccggcaac gaagccatag
      661 acgttcttga aagcatcaaa atgatttatg cctgcatcga gcagagccca ccccttctcg
      721 cgaataatct cacgtaatcg attctcaatc agccgtcccg cgggaagccc ggaacgcgga
      781 tcggtgagac tctcccgctc cgagcgcttg attgcacctt gtacgcgaag tttcagttcc
      841 tcaatatcaa agggcttggt gatgtaatca tctgccccca gttccagacc ctgtaattta
      901 tcgctgcgct catctttttg cgtgaggaaa atgaccggaa tatgactggt acggaggttg
      961 gtacgcaagt tgcggcatac ctcataccca tcaatatccg gtaacataat atcaagcacg
     1021 atcagatgag gcaaagactg ctttgtcttt tccagtgcat ctgacccgcg cacggcgaca
     1081 tcaacatcga attccatacc gctgaaatag atcttcagca tgtttccgat gtcgacatcg
     1141 tcttccacga ccaacaggcg ggcttttccc ataagcggcc tccaaaatta cgtttgtgcc
     1201 gcatgcaatt tgcgagcaag ttttgcattg cggcgtactg tttcgatttg tgcttccgtc
     1261 acttcgcgct cgaacgaacg aaaaccactc aagcggaaac catgtttctt tgcaattgaa
     1321 tcaatttctt ccacacgtgc tctggtcaga tgtttaccca gagtgtaatc ctcaaatcga
     1381 ccctccaggg caagggcaat tgtctcagcc atgcaggcat aggcctttcc ggcgggaaaa
     1441 ccaaagttaa aatggaaatc aacgggtcct ggaacatcaa ccattccgcc gtcaatcact
     1501 aatatatcat ctcttgccgc tgctaccatt gcagaaacat cacgaggacg ggctacatca
     1561 cacacaacac tgccaggttg cagatgctcc ggtcgaatca catcgtggat ggaacttgtg
     1621 accgtcagaa ttagctgtgc gtccgacaaa acatccatcg tggtgctcag gattaattcg
     1681 gctcgtgcgt ggactttaag cctgtcgcgc aattcttcaa gggcatcttg tctgcgcccg
     1741 atcaaatata gccgttccac gtcatcggca agcaggtcag cacaaacctg cccgatcgcg
     1801 cccgttgcgc cgacaacggc agccgtggtt ccctgcagcg ggatatccat gacataggct
     1861 gcttcacgca gagcatccac cgccactgta accgtgtacg agtcacccgt cgtaaccggc
     1921 acatccaaag cattggcaat tgtaatcccg gcatccccta ccacagaggt aaacgcgccg
     1981 agtccaagta tttgtgcacc cagtttttct gccagacgac cagtttcaat gatcttgcga
     2041 tataccgtct gttcgggtaa ctccatcatt cgcctcggcg tatatggaca ggcaatgaac
     2101 catcccttga tttctttgcc ggttgcttca gatttgatcc cttcgatctc agagatgtaa
     2161 actggcggaa agaaggttga gaagaaatcg atctgccttt cattaagaaa acgacctagt
     2221 agaggaaact tgcggcttac atcccgtttt ggatcgatcg gatgaatgat aaatgcaaac
     2281 gtatccacca gttaccaaga ttccagttcg ccctcaccgg gacgcgggta taagcgccgg
     2341 tgcaggctag cagacttaag tttgtggtga tcgctatctt cgtaacggat gtccttgtca
     2401 atgacgcgcc gatctttgga tgcagccagg accacgtctg attcaatcgt gcgagccggg
     2461 tagacgatca tccctgaccc aaggcgacaa ttgtgaccta ctgcactccc gataaccggt
     2521 cggttcgaaa tattcaattt gccttgtcca tccaaagcac gaatcggtgc agggatcaag
     2581 ttgtaatctg taaaggttga accagcgcca ataaatgtat ttcgcccgat gacgcacatt
     2641 tgcagacagg tattttgtgc caccatactg ttttccatca cggtcgtcat gaacagggcc
     2701 gcgcgaaatg gaagaaaggc gccatctccc accacgcaca acatcaattg aacatcctga
     2761 gagatattga cgttattgcc aataatacaa ttctcaatta ctgcaccggc gttgatcgtg
     2821 acattatccc caattgtggt tggtccgtga ataatggcat gtgggtcaat gacacaattt
     2881 ttacctacct tgacgagttc agagcactcc aggatttgcc gcccttcata gatggccttc
     2941 cctaatattt tcagcttaaa acccaaatct tcgccgagac gcttctcaaa ccgtgcgccg
     3001 cgcgaaaaca aaccaaatac gacatcaaca ataaaaatat gtacccacga atcgatcgca
     3061 atcagcgcgc gcagtggtac ttgaaaaaca aggtcaccgc tctggtctgc catgtaggtg
     3121 ggcacatgat agtaaccaat ttcccgagct tcaagattaa taacaagcgg ttccacatcc
     3181 gctacggggc cgcgagggta ataccataaa tcggcaaggt aatgttcacc agcgggagcg
     3241 taggaatttg aaagaggaag cgcgtgttcc ttaaaagc
//
LOCUS       DASTJP010000438         5297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_328217, whole genome shotgun sequence.
ACCESSION   DASTJP010000438 DASTJP010000000
VERSION     DASTJP010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328217"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFQ23_12635"
     CDS             complement(<1..313)
                     /locus_tag="VFQ23_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907490.1"
                     /translation="MIKGDVQSEISGCSVYITMHPHILVTVFMIFWLGFVGFFFLGFL
                     YSLVSSFTEPHTTDLTLLLIPGGMFFFGYAMLLGGFKVESIKSKKFFRDLFEAREVEE
                     MG"
     gene            complement(605..982)
                     /locus_tag="VFQ23_12640"
     CDS             complement(605..982)
                     /locus_tag="VFQ23_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HET9907491.1"
                     /translation="MKNTEKHHQRIANLTFASVYPMYIEKVEKKGRTKAELHQVIEWL
                     TNFDQKKLQQLIKENATFETFFQRASLNPNARLITGVICGVRVEEIDNPLTQQIRYLD
                     KLVDELAKGRKMEKILRQPVASP"
     gene            1068..1283
                     /locus_tag="VFQ23_12645"
     CDS             1068..1283
                     /locus_tag="VFQ23_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907492.1"
                     /translation="MRRLFVEIDFFLSRSTARFSGWRVNNLSFRALRTRQSAARRRSP
                     ERSDGGCVGRGLEDTSLSESQIDLDYG"
     gene            complement(1274..1840)
                     /locus_tag="VFQ23_12650"
     CDS             complement(1274..1840)
                     /locus_tag="VFQ23_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907493.1"
                     /translation="MNEKQCEQRLLAGTQKLDPVLNPLGFVFRISSRGISSPGPYAAG
                     FYEKENKKIGLIYRSISGPGGLGAVIYEDGQVGVDHSTLMRYLGKKGFNKLRYDENRL
                     SSYSYLAGGDVFDALAYDIQNFGVEFLTLSEDQFSITLKEARNTPEPEANPESKEASR
                     RTQKIFVLIGLIILLMNLIPDLINSLQP"
     gene            complement(2566..3045)
                     /locus_tag="VFQ23_12655"
     CDS             complement(2566..3045)
                     /locus_tag="VFQ23_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907494.1"
                     /translation="MLNTIIEYTCTSIFLLVGGYLLYRYVIDRPKNNQAEKVYADYKG
                     ILDSKLIPLGFERKNEYADAREKGITYGRNNLKITLRTEHPFAYTVVHASSGKKITLE
                     ERNNGLPPEIRNKMKYINEQDKNQLVDTADFGIELSGSEEVKAQFLQTIEKWLTENQ"
     gene            complement(3427..4437)
                     /locus_tag="VFQ23_12660"
     CDS             complement(3427..4437)
                     /locus_tag="VFQ23_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9907495.1"
                     /translation="MTSRYSGWLRIALIVGSVMGIVCCIGFGIYWFLLRTTANVVQTS
                     NPWVRSLAWSKGGASLFVGSADRSITYWDVESAKLLYTVNRSASVRDIALSPDGMMLA
                     VGDQTGAINIWYPSTGDVEQLAKEYEQDALGFLAWSPDGSQLAYTGDKGKIMIWDVAH
                     RQKSVFAQAKNGITGISWSPDGTRLATSSTDGTVSIWDRQTGQSIWIIEAHEGWANDV
                     DWALDGSLLASGGDDSQLKVWDAKTGKLVNQTSGSDQIFSVAWSPRANQIAWASAAGQ
                     VAITDFEKPDIHFNAGTGQVWVVAWLRDGQQLAAGTDSNTIFIWETATGQQIQLLTLP
                     MP"
     gene            complement(4681..>5297)
                     /locus_tag="VFQ23_12665"
     CDS             complement(4681..>5297)
                     /locus_tag="VFQ23_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907496.1"
                     /translation="QTSIPTNTVQPTLASIATETPASSTSMGACPTETADLKLFMNAD
                     DGYCLLYPTVHTAIPPYMIVINPTGLAGDMPGDAFEEATQRAIQEERGFRPRDMPGDA
                     WVYINVEAASGRTATQVADGQIAEAGAGWNITRSEILIDGKQAIVVDGLPRPVPLRAV
                     FIVANDRLYTLLFMRMSSNSEVFAQLEELYAVVIDTFHFLPPTP"
BASE COUNT         1362 a         1231 c         1181 g         1523 t
ORIGIN      
        1 atcccatttc ttccacttct ctagcttcga ataggtctcg aaaaaacttc ttcgatttga
       61 tcgactcaac tttaaaccct cccaggagca ttgcataacc aaaaaagaac atgcctcccg
      121 gaattaatag cagggtaaga tcagtcgtat gaggttcagt aaatgacgaa acaagggagt
      181 atagaaaacc caaaaagaag aaccccacaa agcccagcca aaaaatcatg aatactgtaa
      241 caagaatatg ggggtgcatt gtaatataga cagaacaacc gctgatttca ctctgtacat
      301 cccctttaat catgggtaaa aatgaatttc tataaccaat aattcgcgag acttcaaaat
      361 gcgagccttc gatattccct tgatacggtt tcgttcctga cccggagaat cgaaagtggc
      421 gtctgggttc gacggaatcg cctaatcgct ggagtacctc tgcactgctc aatgccgaag
      481 taattttcag tcgttcatac ggcaagaatt tcatccttcc atctctcgtc ttcgttgaaa
      541 ttctatcgat ctgagtgggg tagcgacagg tggatggcgt gtgctcacta atcgcggtag
      601 tttttcacgg cgaagcgaca ggctgccgca gaatcttctc cattttcctg cccttcgcca
      661 actcgtccac caacttatcc aaatagcgaa tctgctgtgt caaaggattg tcgatttctt
      721 ccacacgaac cccacagatt actccagtga tgaggcgcgc attggggttt agcgaagccc
      781 gctggaagaa tgtttcaaag gtcgcatttt cctttatgag ttgctgaagt ttcttctgat
      841 cgaagtttgt taaccactcg atgacctgat gcaattctgc tttggttctg cctttcttct
      901 ccactttttc gatatacatt ggataaacgg aagcgaaggt caggtttgcg atgcgttgat
      961 ggtgtttttc tgtatttttc atgtttacct ttatatcctg tattgtagcg ttattttttc
     1021 gattcgaaca ttttgccaat gactttacaa tctgtcggga tttgcgtgtg cgaagactgt
     1081 ttgttgaaat tgatttcttc ctaagccgct caacggctcg ctttagcggt tggcgggtta
     1141 ataacctatc ctttcgtgcc ttgcgtaccc gccaatccgc tgcacgtcga agatccccgg
     1201 agcgcagcga tgggggctgt gttgggcgtg gactcgaaga taccagcctt tcggaaagcc
     1261 aaatcgacct tgattatggc tgaagagaat ttattagatc agggatcaag ttcattaaaa
     1321 gaatgatcaa gccgattaat acgaatatct tttgagttcg ccttgaagcc tctttggatt
     1381 cagggttcgc ttcgggttca ggagtattgc gggcttcttt caaagtgatg ctaaattgat
     1441 cttcactcaa tgtgagaaat tctactccga aattctgaat atcatacgca agtgcgtcga
     1501 agacatcgcc accagctaaa tatgaataag acgaaagcct attttcatcg tacctcaact
     1561 tgttgaaacc ttttttccct aaataacgca taagggtgct atgatcaacc cccacttgcc
     1621 catcttcata aataacagcg cctaatcccc ccggtcccga aatcgaccga tatattaggc
     1681 cgattttttt attctctttt tcatagaatc ctgcagcata ggggccgggt gatgatattc
     1741 ctctacttga aattctaaaa acaaacccta gtggatttag gacggggtca agcttttggg
     1801 ttcctgctag caaacgttgt tcacattgtt tttcattcat gcagaagctc cttactctag
     1861 ctgctgggtc gataggcatt tcaaaactat actttggatg tgtggttact ccaaatgttt
     1921 acgggcttca tttattgcat tcacaatgtt ctcaacacgc gatttatcct ctgattgata
     1981 aatagatata ttttttgatt gataaataat tcttacattg tatgtcgaag gtgttgatgt
     2041 tctggatgtg tagacgaccc atcctactaa gatgaatcca agtattgaaa aggctatgag
     2101 atcaccatta atagtaaagg tacagccgaa cgcgatcatt agaaggccaa tcactccgac
     2161 tataccccgc gcaattttag ctggtacggg tgaaaaagaa agcatcatag cttgtgcaat
     2221 atcggatgta ttgtaggatt ttccaaatat ggttaccttt agattcgtaa cttttatgtt
     2281 cttgctcttg aaaaatgttg tttcattaac gtttttcata gcatcccaga ccttttacca
     2341 tagtgtaatc tgactaacgg ctagcgttat tggcagtggg cgggctgaga ctagccgtcg
     2401 aaacaggaag gactcaaagc cgcgaaaatg cttgaatacg cggcgcgtac ctacctgccc
     2461 actgctcgtc gaagatcccc ggaccgcagc gatgggggcc atgctgggct gtttatgatc
     2521 ccgaagactc gtgaaaatat tgttgcgcca atggtggaac aagaattatt gattttcggt
     2581 tagccatttt tcaattgttt gtaaaaattg tgcttttacc tcttcggatc ccgaaagctc
     2641 tatcccgaaa tcggctgtgt caaccaactg gtttttatct tgctcattta tgtacttcat
     2701 tttatttctg atctctggcg gtagtccgtt gttacgttct tctaatgtaa tctttttgcc
     2761 gctgcttgca tggactacag tgtaggcaaa aggatgttca gtgcgcagcg taattttgag
     2821 attgtttcga ccataagtaa ttcccttctc cctcgcatct gcatactcat ttttacgctc
     2881 aaagcccagt ggtattaatt tgctgtctaa aattcctttg taatcagcgt agactttttc
     2941 cgcctgatta ttttttggtc tatcaatcac gtatctataa agcaaatacc cacctacaag
     3001 cagaaaaatt gatgtacaag tatattcaat tatagtgttc agcatattat ccactccttt
     3061 gagtcgttac tgcggggtga aagatttgtg attgcgaaat agcttctcat taggttgtcg
     3121 ctatctcctc tccatagatg gtacaaacat tgaaattcct gtcgagacta atttctgcgg
     3181 gtactttttc tagtttaccg cccaacggtg agcgttaccc gtcgcgtagc accctggtga
     3241 gggctggtgg gcgggttgag ataacgcgat tttgacggaa ccaacttcaa gccacaaaaa
     3301 gtgcctgaaa acgcgcagac tcccagacgt cgggtgcacg cattgttcgg cggttttgga
     3361 atttgaacgg aatacacaaa gccttcgtaa aatacacatg tcaccaattg gcgtagtttt
     3421 gcaatattac ggcatcggta aagtcagaag ttgaatctgt tgacctgtcg cggtctccca
     3481 tatgaaaatc gtgtttgaat ctgtaccagc agcaagctgt tgtccgtctc gcaaccacgc
     3541 tacaacccac acttgacctg ttccggcgtt gaaatgtata tcgggtttct caaagtccgt
     3601 aatggcgact tgaccagcag cgcttgccca tgctatctga tttgctctag gcgaccaagc
     3661 tacgctaaat atctggtcag agccgctggt ttggttaacc aatttgccgg tttttgcatc
     3721 ccaaactttg agttggctat catcccctcc agaagcaagc aagctaccat caagtgccca
     3781 gtccacatcg tttgcccagc cttcatgggc ctctatgatc caaatggact gacccgtctg
     3841 acgatcccaa atgctcaccg ttccgtcagt tgaggaagta gccaagcgag tgccgtcagg
     3901 tgaccaggaa atgccagtaa taccattttt tgcttgtgcg aaaacagact tttgtcgatg
     3961 ggctacatcc catatcataa tttttccctt gtctccggta taagctagtt gagaaccatc
     4021 cggcgaccag gcgaggaatc ctaaggcatc ctgctcatat tctttagcta gttgctccac
     4081 atcacctgtg gagggatacc aaatgttgat agcaccagtt tggtcaccta cagccagcat
     4141 cattccatct ggcgataatg ctatgtctcg tacagacgca ctgcggttaa cggtgtacaa
     4201 aagttttgca gactcgacgt cccaatacgt gatggagcga tctgcggagc caacaaagag
     4261 gctggcgcca cctttcgacc atgcaagtga tcgtacccag ggattgctgg tttggactac
     4321 attagctgtc gtacgaagga ggaaccagta aattccaaac ccaatacaac acacaatccc
     4381 cataacggac cctacaatca gggcaatacg caaccatcct gaatacctgc tggtcatatt
     4441 acctctcttt cgcggtgtac ggtaagacga tagccatcgt taatggtgtg ataagagaca
     4501 gacttcaacg ttgaaccgcc gaacggctgg cgttaccggc ggctggggag aaacaactta
     4561 ctaaacggaa aactgccaaa gctcagaaca atctccaaaa aacgcgcaga ctcccaccag
     4621 tcgggtgcac gctgtgttgg ctggcgtttt ggttttctga gtgagaagtc tcatcttttc
     4681 ttatggtgta ggtggtagaa aatggaaggt gtcgatcact actgcgtaca attcttctaa
     4741 ttgagcaaag acttcactgt ttgacgacat acgcatgaac aagagtgtgt atagacggtc
     4801 gttggcaaca ataaaaactg cacgcaaagg aacagggcga ggaagaccgt ccactacaat
     4861 tgcctgtttt ccatcgatca gaatttcact gcgtgtgatg ttccagcccg ctcccgcttc
     4921 tgcaatttga ccatctgcca cttgggtagc agtacgaccg gatgctgctt ctacgttgat
     4981 atatacccaa gcgtctcctg gcatatctcg cggccgaaag cccctttctt cctgtatggc
     5041 gcgctgggtc gcttcttcga aagcgtctcc tggcatatct ccggccaagc cagttggatt
     5101 gatgacaatc atatagggtg ggattgcggt gtgcactgtg gggtagagga gacaatatcc
     5161 atcatcggcg ttcatgaaaa gtttcaaatc tgccgtttca gtgggacatg cgcccatgga
     5221 agtagaacta gcgggcgttt ctgtcgcgat ggatgccaat gtgggctgta cagtgttcgt
     5281 ggggatggat gtttgaa
//
LOCUS       DASTJP010000439         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_952922, whole genome shotgun sequence.
ACCESSION   DASTJP010000439 DASTJP010000000
VERSION     DASTJP010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952922"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..500)
                     /locus_tag="VFQ23_12670"
     CDS             complement(96..500)
                     /locus_tag="VFQ23_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9907497.1"
                     /translation="MEKKLQLIIFMIGILLLVACGTSQTEGEATLASVPTEFAGLTNP
                     FGPEAGSAGAEVFQANCELCHGPQGHGDGVAGESLEPKPKNLAELQTMVGDDYLFWRI
                     YEGKPGTSMVAWRGILTDEQIWQVVSFIRTLK"
     gene            complement(625..1038)
                     /locus_tag="VFQ23_12675"
     CDS             complement(625..1038)
                     /locus_tag="VFQ23_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9907498.1"
                     /translation="MTSLPRAKLFWRMNVMQEALIIDDNRTTADALSQMLKVLGFKSR
                     VAYGSRAAMSVLGTGFTPMFVCLDINMPGLDGTEILAYLRREPRLIPVPVFIITSDDQ
                     PETRRKVMKLGATVMIIKPATIDALEDALKKAKLS"
     gene            complement(1082..2425)
                     /locus_tag="VFQ23_12680"
     CDS             complement(1082..2425)
                     /locus_tag="VFQ23_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9907499.1"
                     /translation="MTNQATATWIEKDKRQLHPIYHPKSHAAPLVIERGEGVWLYTTD
                     GQKILDGMAGLWNVSVGYGREELAQAAYDQMKEMAFTSNFAGMTNLPSIQLADKLAGF
                     AYEGLNTTFFTSGGSEANDTAFKTARYYWKRKGKPTKYKVIARRGAYHGVTLAATFAT
                     GLEKYHTMFGPAVDGFVHIPAPNPYRFDGELKSGETVGQAAARALEEAILREGADTIA
                     AFIAEPVMGVGGVIVPPDDYFPLVRAVCDKYEILFIADEVITGFGRTGEWFALKHWGV
                     KPDILSFAKAITSGYAQLGGIQISDEIRATMESAAESETYMHGYTYSGHAMACAVGVK
                     NLELMEKENLPARSKELGKRLLEGLKTLSEFSFVGDVRGLGLLCGVEIVSNKESRSPD
                     AALAMKIFKAAQERGLRTRPVGTALAFSPPLSINEDEVDEIVKRLGAAMDSMSST"
     gene            complement(2475..>2928)
                     /locus_tag="VFQ23_12685"
     CDS             complement(2475..>2928)
                     /locus_tag="VFQ23_12685"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET9907500.1"
                     /translation="YGVPIIADGGIRQPGDVVKAIAAGAHTVMIGSMLAGTDESPGMI
                     MTRRGHRYKASRGMASLEANINRNRREGNDLTHEEIEDYVAEGVEAAVPYRGRAREVL
                     NQLVGGLQSGMSYSGAHSLDELQEKAIFVRMTGAGLRESGPHDVEVIG"
BASE COUNT          706 a          846 c          645 g          731 t
ORIGIN      
        1 ccgcaggaag cggcacggct ctctcctcgt caggttgggg ctattataaa caatattttc
       61 ggtgagtagg atatttattg aaaaactgaa tgttattact tcaaggttcg aatgaaggaa
      121 acaacttgcc agatttgctc atccgtcaga atgcctcgcc atgcaaccat agaggtcccg
      181 ggcttgccct cgtaaatccg ccagaacagg taatcgtcac cgaccatcgt ctgtaattct
      241 gccagatttt taggcttggg ctcaagcgat tcgccagcca caccgtcacc atgaccttgc
      301 ggcccatgac acagctcaca attggcctgg aatacttcag cacccgcgga accagcctcc
      361 ggaccgaacg gattggtcag accggcaaac tcagtcggga cagaagcaag ggtagcctcg
      421 ccttccgtct gggatgttcc gcaggccacc aacagcagga tacctatcat gaaaataatg
      481 agttgcagtt ttttttccat atactccaca agattcaatc tttttcgaat agattatgct
      541 gtgttgtgaa aaccattaaa accgtccgat aaaaaagaga ccgtccacag cggcggtctc
      601 atgaattacg agacggacga attactatga cagcttcgcc tttttcaagg catcttccaa
      661 cgcatctatc gtcgctggct tgataatcat tacggtcgcg ccaagcttca tcacttttct
      721 tctcgtctct ggctggtcat ctgacgtgat aataaacact ggcacgggga tcaggcgtgg
      781 ttcacgtcga agataggcaa ggatctccgt gccatccagg cccggcatat tgatatccaa
      841 acacacaaac atcggtgtaa aaccggtccc caatacagac atggccgctc gggagccata
      901 ggccacccga gatttaaacc ccaacacttt caacatttgg ctaagggcat cggcagtggt
      961 gcggttatca tcaatgatca gtgcttcctg catcacattc atcctccaga ataattttgc
     1021 ccggggcaga gatgtcataa cccatcctcc aagccaccgg cttcaatgca gaatcagctc
     1081 atcaagtact gctcatacta tccatcgccg cgccaagccg ctttacaatt tcatccactt
     1141 cgtcctcatt gatcgaaagt ggaggtgaga aagccagtgc agttccgacc ggccgcgtcc
     1201 gcaatccgcg ttcctgggct gctttaaaga ttttcattgc cagtgcagca tcgggagacc
     1261 ggctctcttt gttcgagaca atctccactc cgcacagcag ccctaaaccg cgcacatccc
     1321 cgacaaatga gaactcagat aaagttttca ggccttccag cagacgcttt ccaagttcct
     1381 ttgaacgtgc tggaagattt tccttttcca tcagttcaag gtttttgact cccactgcac
     1441 aagccatcgc gtggcctgaa taagtgtagc catgcatgta cgtttcgctt tctgcagcag
     1501 attccattgt cgcgcggatt tcatctgaga tttggattcc gcctaattga gcgtacccac
     1561 tcgtaatggc tttggcgaat gacaggatat ccggtttgac tccccaatgc ttcagtgcga
     1621 accactcacc ggtgcgacca aaccctgtga tgacttcatc cgcgataaaa aggatttcat
     1681 acttatcaca aacggcgcgc accaggggga aataatcatc cggcgggacg atcactccgc
     1741 ctacccccat caccggctct gcaataaacg ccgcgatcgt gtcggcacct tctcgcagaa
     1801 ttgcctcttc cagcgcccgc gctgccgcct ggccaacggt ttcacccgat ttcaattcac
     1861 cgtcaaaacg gtatggattc ggcgcgggga tatgaacaaa cccatccacc gcaggaccaa
     1921 acatcgtatg atatttttcg agaccggtcg cgaaggttgc cgcaagagtt accccgtgat
     1981 atgcccctcg acgagcaatc accttatatt ttgttggctt tcctttccgc ttccaataat
     2041 aacgtgcggt cttgaaggcg gtgtcgttgg cttcagaacc accggaggta aagaaagtcg
     2101 tattcaagcc ttcatacgca aaaccggcaa gcttatccgc taactggatc gatggcagat
     2161 ttgtcatccc ggcaaaattg gaagtgaaag ccatttcttt catttggtca taggccgcct
     2221 gcgcaagttc ctcgcgccca tatcccacgc ttacgttcca taaacctgcc atcccatcca
     2281 gaattttttg cccgtcagtc gtatagagcc aaaccccttc cccacgctca ataactaaag
     2341 gtgccgcatg agatttcgga tgatagatgg gatgtaattg tctcttatcc ttttcgatcc
     2401 aagttgctgt agcctggttg gtcatgttgt ctccgtttta gtcgtcattc cgattgaagt
     2461 atcaggaaat cggatcatcc aattacttcc acatcgtgcg ggcctgactc cctcaatccc
     2521 gcgccggtca ttctcacaaa aattgctttc tcttgcaact catccaaaga atgtgcaccg
     2581 ctatagctca tacctgattg tagcccaccc accaattgat tcaacacttc gcgtgccctg
     2641 ccgcgataag ggacagcggc ttccacacct tctgcaacat aatcctcgat ttcctcatgg
     2701 gtcaagtcgt tgccttcgcg tcgattgcga ttgatattgg cttcaagcga tgccatcccg
     2761 cgcgaggctt tataacggtg tcccctgcgg gtcatgatca tgccagggct ttcatccgta
     2821 cctgccagca tggaaccaat catcaccgtg tgcgcaccgg ccgcgatggc ctttacgacg
     2881 tcacctggct ggcgaatccc tccatctgcg atgattggaa cgccatac
//
LOCUS       DASTJP010000440         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_935566, whole genome shotgun sequence.
ACCESSION   DASTJP010000440 DASTJP010000000
VERSION     DASTJP010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935566"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFQ23_12690"
     CDS             <1..707
                     /locus_tag="VFQ23_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET9907501.1"
                     /translation="IRKTLEVSVLFPQVQIVQLPQPAPDLIGATVIVSGSGCRLRSIA
                     EEPVSNMACIIVDVDGKTGRMFSAPSRHFQRVTSEFNSHLAIHVDQMRARDMQLHTVV
                     HGQPIHLTYLSHISRYQDWRYLNNHLLRWQPETIINFPDGFGVLPFIMPGSEEQMSAT
                     VQVMRASKMLLWSQHGVVARSDDSILHALDLIEYAETAAHYEYLNLLADEKSDGLSPQ
                     QIRSICEFWNVRQSVF"
     gene            1000..1437
                     /locus_tag="VFQ23_12695"
     CDS             1000..1437
                     /locus_tag="VFQ23_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9907502.1"
                     /translation="MATVRDMIRRKGEEVFSVAPDATVLEAIRLMAEHNIGALLVMTE
                     GDADGIVSERDCMRKVELEGKSSKDTPVSTIMTRKLITVNCDQPLEECMELMLDKNIR
                     HLPVYDGKKLVGVLSVRDVLKEVVNVQKIMLSELERYITGGGR"
     gene            complement(1854..2180)
                     /locus_tag="VFQ23_12700"
     CDS             complement(1854..2180)
                     /locus_tag="VFQ23_12700"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9907503.1"
                     /translation="MAKATPELKRVVKRYRLQLEKMGIHPTRVLLYGSQAAGTAREGS
                     DIDLIVISGDWKKYNHRQRLELLGIAAARILEPVQAQGFTPSEIRSKKVTAFWQQVIK
                     EQAIAI"
     gene            complement(2147..2539)
                     /locus_tag="VFQ23_12705"
     CDS             complement(2147..2539)
                     /locus_tag="VFQ23_12705"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HET9907504.1"
                     /translation="MRKDTANWVALADYDIETANHMLATERYLYVIFLCHLALEKLLK
                     AHVAEVTQTVPIKTHDLIYLVKKCELDLPENHLDFIGKINTASIPTRYPDDLQRALKE
                     YPKSVAKNYLKQTTELVQWLKQHQNLSE"
     gene            complement(2782..>3552)
                     /locus_tag="VFQ23_12710"
     CDS             complement(2782..>3552)
                     /locus_tag="VFQ23_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907505.1"
                     /translation="EQEQQRQKEQQIELEKKLGLRLRRLTYFLIGVFTIAFIALFLWL
                     DRVVGDLAVNSVQDQMLAIAETAISFVDGDEYERLINSYEEDDVAVLEDEYYQWLAAI
                     VDRIVSANENVDGDMGIYFVAPTSGENQVLAIYADFDNFKAVWDVGSHTVMLNGMNAT
                     SANTQPFDDPYGTWVSACTPILNSDENSVGALCVDLEASIVRETRRQSTNILWTAFLV
                     IYPAMILVVILTTRSLSRARLKAIQTQPEIAATELGPS"
BASE COUNT          884 a          817 c          892 g          959 t
ORIGIN      
        1 gtattcgtaa gacattggaa gtcagcgttc ttttccctca ggtgcaaatc gtccagcttc
       61 cccagcctgc tccggatctt atcggcgcaa cggtaatcgt ttccggctca gggtgtcgct
      121 tgcgatcaat tgcagaagaa ccagtatcca acatggcctg tatcattgtt gacgtcgatg
      181 gaaagactgg cagaatgttc agcgctccca gccgccattt tcagcgtgta accagcgagt
      241 tcaattcgca tttagcgatc catgtggacc aaatgcgcgc gcgcgacatg caactgcata
      301 cggtcgttca cggtcagccc attcatctga cgtatctcag tcatatctct cgctaccagg
      361 attggcgata tctaaacaat catcttcttc gctggcagcc agagacgatc atcaattttc
      421 cggatggctt tggcgtcctg ccattcatta tgccagggtc tgaagagcaa atgtctgcga
      481 cggtgcaggt catgcgcgcc agtaaaatgt tgctctggtc acagcatggc gttgtcgcga
      541 gaagtgatga ctccattctg cacgccctgg atttgatcga atatgccgaa acggcggcgc
      601 attacgaata tttgaacctg cttgcagatg aaaaaagcga cgggctcagc cctcagcaga
      661 tccgttctat ttgtgaattt tggaatgtgc ggcaaagcgt cttttgaccc gcttcttttt
      721 cgacgaattg gtgtttgttg atatagcaag tacgagcaag gtaacttgcc cctacttaag
      781 gctttctcaa ggttgccgtg agatttgcaa gcccacacaa ttaaaccgct aagcacgcga
      841 agcccgcaaa gttttacgat tttcttggcg ttcttagcgg gcttcgcggt tcagagactt
      901 tgtctgacac taatttgaga aagtcctggc ccctacttgc aagactgccc atttgagtct
      961 atactctaat caatcttttt cacaagagga gaggcagcca tggctaccgt tcgagatatg
     1021 atccgtcgga aaggggagga agtcttctcg gttgcacctg atgccacggt gttggaggcg
     1081 atcaggctga tggcagagca caatatcggt gcgttgctgg tcatgacgga gggggatgcg
     1141 gatgggatcg tttcagaacg tgactgcatg cgcaaagtgg aactggaggg gaaaagttcg
     1201 aaagatacgc ctgtttcaac gatcatgacc aggaaactca tcacggtgaa ttgtgaccag
     1261 ccgcttgagg aatgtatgga gctcatgctc gataaaaata tccgtcacct gccagtgtat
     1321 gatggaaaga aattagtggg cgtgctctcc gtccgtgatg tgctcaaaga ggtagtgaac
     1381 gttcagaaga taatgctctc tgagctggag cgttatatca caggcggcgg acggtagctt
     1441 gttcatgttc ttacagtagt tgaactgctg taagaacgag gaaactggat gaggttgcct
     1501 gagtggacaa agctgaactg caggaaaaga tgaaatccgg cgagttgacc gcgcgtcaac
     1561 tcgctgtttt gtatctgggc cagatcgatg ccgttgacaa aagtggtccc gcgtgaatga
     1621 tcgacctcgt caatgacgat gcaaacatct gggatatgga aagccgttcg gcccctcccg
     1681 ccgctatcgc ggcaccctcc tcaagtccga aaataggttg gttcataatt tttcaattct
     1741 tcaaattcga acttggggag ggacgggatg cggcttcaaa gatctgttcg tcggatttgg
     1801 aagagaagag gtaagtgaaa ttttggacga aaatcggagc cattcgactc cgattagatt
     1861 gcaattgcct gttcttttat tacttgttgc caaaacgccg ttactttttt ggatcgtatt
     1921 tcgctggggg taaatccttg tgcctgaaca ggttcaagga ttcttgccgc ggcgattccc
     1981 aacagctcta gtcgctgacg atgattgtat tttttccaat cccccgaaat cacgatcagg
     2041 tcaatgtcac tgccttcgcg tgccgtgcca gcagcttggg agccgtaaag cagaacacgc
     2101 gtgggatgga tccccatttt ttcaagctgc aagcgatagc gtttaactac tcgcttaagt
     2161 tctggtgttg ctttagccat tgaacaagtt ccgtagtttg ttttaaataa ttcttggcga
     2221 ccgattttgg atattccttt aatgcgcgtt gtaagtcatc agggtaacga gtaggaatgc
     2281 tggctgtgtt gattttacca atgaaatcaa ggtggttttc tggtaagtca agttcacatt
     2341 tcttcaccag gtaaatcaag tcatgcgttt ttatggggac ggtttgagta acttctgcaa
     2401 catgagcttt gagtaatttc tccagcgcaa ggtggcaaag aaagatgaca tacagatatc
     2461 tttctgttgc caacatgtga ttggcagttt caatatcgta atcagcgagt gcaacccagt
     2521 tggcagtgtc tttgcgcaca gttgaaaacc tttcctaaat tataccctct gcgtttcagg
     2581 tagaaatgaa aacagtctat tcgatgagcc ccgtcatgcg aggtctcctt ggaggggctt
     2641 ataaaagaaa aggataaaga cctgacaggt ctcactgcgc ggttgttcac aaagccgcat
     2701 gcgtggtcaa agacaccgcc tgacgaaatt tctcggagac ctgtcaggtc tattgaattt
     2761 tggccttgcg caaggaaggt actaagacgg cccgagttct gttgcagcaa tctctggttg
     2821 agtttggatg gctttgagac gtgctctgga aagcgagcgt gttgtaagga tcactaccag
     2881 aatcatcgcc gggtagatta ccaaaaaagc tgtccatagg atgttggtgg actggcgtcg
     2941 tgtttctcgt actatggaag cctcaaggtc cacacataac gcaccgacag agttttcgtc
     3001 cgaatttagg atgggcgtgc aagctgagac ccaggtgcca taaggatcat cgaatggctg
     3061 ggtgttcgcg ctggttgcat tcattccatt tagcataacg gtgtggctcc caacatccca
     3121 tacagcctta aagttgtcga agtctgcata aattgccaga acctgatttt caccgctggt
     3181 tggcgcaaca aaatagattc ccatgtcacc atcgacattt tcgttggcag acacgatacg
     3241 atctactatt gcagcgagcc attgataata ttcatcctct agcactgcta catcgtcttc
     3301 ttcataagag ttgatcaatc tctcgtattc atctccatcc acaaaagaga tggcagtctc
     3361 tgcgatagcc aacatttgat cctgtactga gtttacagcg agatctccca ccactctgtc
     3421 cagccagagg aataaagcaa taaaggcaat tgtaaaaacg ccgatcagaa aataagtaag
     3481 ccgccgtaaa cgcaagccta atttcttctc cagctcgatt tgttgctctt tttggcgctg
     3541 ctgttcctgt tc
//
LOCUS       DASTJP010000441         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_11606, whole genome shotgun sequence.
ACCESSION   DASTJP010000441 DASTJP010000000
VERSION     DASTJP010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11606"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /gene="murA"
                     /locus_tag="VFQ23_12715"
     CDS             <1..1261
                     /gene="murA"
                     /locus_tag="VFQ23_12715"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9907506.1"
                     /translation="GGTPINGEITPAGNKNAALPLLAACLLTEEPVILRNIPQIKDVN
                     VMRQLLESLGASVEDVDASSWRITARELRPADLNPDLCRQIRASILIAGPMAARIGKF
                     KLPSPGGDVIGRRRLDTHILALRALGADVTYDRGFSFQANGLHGADFLMDEASVTATE
                     NAIMAAVTARGDSVIHNAASEPHVQELCLFLNLLGAQIDDIGSNTLHIHGVPKLHGGE
                     FTIGPDYIEVISFVGAAAVTRGSVRIKNAGVKNLKMIHHIFERMGVTWEVEGDDLIVP
                     AEQELTIIPDLDGAIPEVKTNVWPAFPTDLMSIAITVATQANGSIMFHDWMFSGRMYF
                     TDKLVGMGAKIILCDPYRCLIQGPNQLYGENLESPDIRAGMSMLLAALAAKGQSTIRN
                     IGQIERGYQDVDKKLRLLGANIVRMQE"
     gene            1368..2309
                     /gene="trxB"
                     /locus_tag="VFQ23_12720"
     CDS             1368..2309
                     /gene="trxB"
                     /locus_tag="VFQ23_12720"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET9907507.1"
                     /translation="MSSNGNAKHVKVLVLGSGPTGYSAALYAARAELQPVVLTGMQLG
                     GQAALTYTIENYPGFPDGVGGAQLGELFQKQAEHFGAVTEFASANSVDLSQRPFRVES
                     DNGDYLAESLIVGTGASPNHLNIPGETELTGRGVSYCATCDGWFFKDKKVVVVGGGDS
                     ALEEGLFITRYASSVTIIHRRDEFRASPILQNRAKEHPKINFILDTIVTDVIGTDKLE
                     ALKLKNVKTGDEAIYETDGLFIFIGHTPNTGMFQGQLDMSDLGYITINEKMETNVPGV
                     FAAGEAADPHFRQVVTSAGMGAAAAIQATRFLESESG"
     gene            2485..3420
                     /gene="mdh"
                     /locus_tag="VFQ23_12725"
     CDS             2485..3420
                     /gene="mdh"
                     /locus_tag="VFQ23_12725"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9907508.1"
                     /translation="MNKISIIGAGMTGATTAHWLAEREIADLVLVDVVEGMPQGKGLD
                     LLEAMPVIGRDVNVVGSNDYSATHDSDIIIITAGIARKPGMSRDDLLKTNSEIVGNAA
                     TETLKHSPNAIYIILTNPLDTMAYLAMKKTGLPRERVIGQAGILDSARMRAFVALETG
                     VSVENIDCYVLGGHGDEMVPLTRHSNIAGVALKEYVPADKLSAIVERTRKGGGEIVNL
                     LKTGSAFYAPAAACAQMAEAILKNKHLIVPCAAYMNGEYGLNDMYFGVPVMLGRGGLE
                     RIIEYKLDEEEKAMFDKSAAAVKETHEALKNLVKF"
     gene            3474..>4472
                     /locus_tag="VFQ23_12730"
     CDS             3474..>4472
                     /locus_tag="VFQ23_12730"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HET9907509.1"
                     /translation="MILHEKISEQLPKWRDRIRSLAKEHADVVVDTVTIGEVVGGMRD
                     TKSLLTDISYVDPAEGIRFRGMTIPEVLKALPKARGGKMPMVGGLYYLLIVGEVPTKE
                     QALDVEAEWAKRATVPDYVFKMLKTMPKETHPMTLLSQAVLALQTESVFARKYHSMKK
                     DAYWDAALEDSLNLTAKLPLVAAFIYRMKYFGETGKPKYNPRQDYGLNFARMMKVSNK
                     KEYADLMRLYFIIHSDHESGNASAHATHLVGSTLSDIYYSFSGGLNALAGPLHGLANQ
                     ESLAWLLDVHKQFNGVPSRDDLYKFAWDTLNSGHVIPGYGHGVLRVIDPRFVTQMEF"
BASE COUNT         1151 a          963 c         1249 g         1109 t
ORIGIN      
        1 aggcggaact ccaattaacg gagagattac acctgcagga aacaaaaatg ctgcgcttcc
       61 cttgttagct gcctgcctgc tgacggagga gccagtgatc ttgcggaaca tcccgcagat
      121 taaggatgta aatgtgatga ggcagctctt agaaagcctg ggcgcgagcg ttgaggatgt
      181 agatgccagc tcgtggcgta tcacggcgcg ggaactccgg cctgcagatt tgaaccctga
      241 cctgtgccgc caaattcgcg cgtccatctt aattgcggga ccaatggcag cgcgcatcgg
      301 aaaatttaag ctgccatctc caggggggga tgtgattggt cgacgacgat tggatacaca
      361 tattcttgcg ttgcgcgctc tcggtgcgga cgtcacgtat gatcgaggtt tttcttttca
      421 agccaatggt ctacatggcg cagatttttt gatggatgaa gccagtgtca ctgcgacgga
      481 aaatgccatc atggcagccg tgacagccag gggagactca gttattcaca atgccgcttc
      541 tgaacctcat gttcaggaat tatgtctttt tctaaattta ctgggtgcac agattgacga
      601 catcggttcg aatacgcttc atattcatgg cgtgccaaag ctgcacggtg gcgaatttac
      661 gattgggccc gactatattg aagtgattag ttttgtgggg gcggcggcgg tgacgcgtgg
      721 cagtgtgcgg attaagaacg ctggtgtgaa aaatttgaaa atgatccatc atatctttga
      781 gcggatgggg gtgacctggg aggtcgaagg cgacgacctg atcgtccctg cggaacagga
      841 gcttaccatc atccccgatc tggatggcgc aattcccgag gtcaaaacaa acgtttggcc
      901 cgcttttccc acggacctga tgagtattgc gatcaccgta gccactcagg caaatggatc
      961 catcatgttt catgactgga tgttttcagg acgtatgtat ttcaccgata agctggtggg
     1021 catgggcgca aagattatcc tatgcgaccc ctatcgctgt ttgatccagg gaccgaatca
     1081 actgtacggt gaaaatttgg aaagcccgga tatccgcgcg gggatgtcga tgttattagc
     1141 tgcattagcc gcaaagggac aatccacaat caggaatatc gggcaaattg agcgcggcta
     1201 tcaggatgtg gataagaagc tgcgtttgct gggtgcgaat attgtaagga tgcaggaatg
     1261 aaaactgctt catcaaatga agtctttaag acagtttcac tgtaaaatac tggtgaaacg
     1321 agtcgtcttg attgtgaaac aatattaagc gaggaaatga ctaagttatg tcttcgaatg
     1381 gaaatgcaaa acacgtaaag gtattggtcc tggggtcggg gcctacaggc tattcagcgg
     1441 ctttgtatgc ggcacgcgcg gaacttcaac cggttgtgtt gaccggtatg caattgggcg
     1501 gtcaggccgc gctgacttat acaattgaaa actatcccgg attcccggat ggggttggag
     1561 gcgcgcaact tggcgagttg ttccaaaaac aggcggaaca cttcggggct gtgaccgagt
     1621 tcgcttccgc caattcggtg gacctatctc aacgtccgtt ccgagtcgaa tctgataacg
     1681 gcgattatct ggcagagtca ctgattgttg gcactggcgc gagtccaaat cacctcaata
     1741 tccctggtga gacggagcta acaggtcggg gcgtttccta ctgtgcaact tgtgacgggt
     1801 ggttcttcaa ggataagaaa gtggttgttg ttggcggagg agatagcgcg ctggaggaag
     1861 gattgtttat cacacgatat gcctcgtctg tgacgataat tcacaggcgc gatgaatttc
     1921 gtgcaagccc aatcttacaa aatcgagcga aggaacaccc gaaaatcaat ttcatcctgg
     1981 acacgatcgt cacggatgta atcggcactg ataagctaga agcgttgaaa ttgaagaatg
     2041 tcaaaacggg tgacgaagcg atctatgaaa cggatggcct attcattttt attggtcaca
     2101 ccccgaacac ggggatgttc caaggtcaat tggatatgag tgacctggga tatataacga
     2161 tcaatgaaaa aatggaaacg aatgtacccg gggtttttgc ggccggggag gcggctgatc
     2221 cgcatttccg gcaggtcgtc acatccgccg ggatgggtgc tgcggctgcg attcaggcga
     2281 ctcgtttcct tgaatcagag tcgggctgag atgagtcaga tcatgtatga tcgagtttat
     2341 gtaagcccaa attgacagga taatgaacaa gacttccgag gtggaagtct tgttttctaa
     2401 gtattacagt aacatcaatt tgcactgata ggtctgaaat tttcggataa aattgattgg
     2461 atacatatct tggatggagg ataaatgaat aagatttcaa tcattggcgc aggcatgacg
     2521 ggcgccacga ccgcgcactg gcttgccgaa cgtgaaattg ctgaccttgt tttggtggat
     2581 gttgtcgagg gaatgccgca gggtaagggg cttgatctcc tggaagcaat gccggtgatt
     2641 ggaagagatg tgaacgtggt tggctcgaat gattattccg ctacgcatga ttccgatatc
     2701 atcatcataa ctgcggggat tgctcgaaag ccaggcatga gccgtgatga tttgttgaaa
     2761 acaaattccg aaatcgttgg caacgccgcc acagagacac tcaaacattc acctaatgca
     2821 atctacatca tattgacgaa tccgctggat acgatggcct atcttgcaat gaaaaagacc
     2881 ggactgccgc gcgaacgcgt tattgggcag gcaggcattt tggattcggc gcgaatgaga
     2941 gccttcgttg ccctggaaac aggcgtgagt gttgaaaaca tcgattgtta tgtattaggt
     3001 gggcatggcg acgaaatggt ccctctgaca cgtcactcga atattgcagg cgttgcacta
     3061 aaggaatatg tccccgccga caagttgagt gcaatcgtcg agcgcacgcg caaaggagga
     3121 ggcgagatcg taaacctgct taagaccggt tctgcatttt atgcacccgc ggcggcctgt
     3181 gcgcagatgg cagaagccat tctcaagaac aagcatttga tcgttccctg cgcggcgtat
     3241 atgaatgggg aatatggttt gaacgatatg tactttggcg tgccggtcat gttaggcagg
     3301 ggagggctgg agcggattat tgaatacaag ctggatgaag aagaaaaagc catgtttgat
     3361 aagtccgctg ctgctgtgaa ggaaacgcat gaagcattaa agaatttagt gaagttttag
     3421 catcgcttac tcccgtcctt tctgagagaa ttgacggatt attggaggcc tttatgatcc
     3481 tgcacgaaaa aatttctgag caattaccca aatggcgtga tcggattcgc tcacttgcca
     3541 aagagcatgc ggatgtcgtg gtagatactg tgacaattgg agaggtcgtg ggtgggatgc
     3601 gcgataccaa atctctgctg acagatatct cctatgtgga cccagcggag ggtatccgtt
     3661 ttcgagggat gacgatccct gaagtcttga aggcgttacc gaaagcgcgc ggtggcaaga
     3721 tgccgatggt gggaggctta tattatcttt tgatcgtggg cgaagttccg accaaggaac
     3781 aagcattgga tgtggaagcc gaatgggcga agcgcgctac cgttccagat tacgttttca
     3841 agatgctgaa gacaatgccc aaggagactc atcccatgac tctcctgtcg caggctgtgc
     3901 tggcattaca aacggaatcc gtctttgcac gaaaatatca tagtatgaag aaagacgcgt
     3961 attgggatgc ggcacttgaa gacagtctca atctcactgc gaagctaccc ttggtcgccg
     4021 cgttcatcta tcgcatgaaa tacttcggag aaacgggcaa gccaaaatac aatcctcgtc
     4081 aggattacgg tttgaatttt gcacggatga tgaaggtttc caataaaaag gaatatgcag
     4141 atttgatgcg cctttacttc atcattcaca gtgatcacga gtcgggtaac gcatcagcgc
     4201 acgcgaccca tctggttggt tccacactgt cggatatcta ttactccttt tcaggcggat
     4261 tgaacgcgct cgcgggaccg ctccatggac tcgcgaatca ggaaagcctc gcctggctgt
     4321 tggatgtgca caagcaattt aatggcgtac cttcacgaga cgatctatat aaatttgcat
     4381 gggatacact taacagtggg catgtaattc cgggttatgg acacggtgtt ctgagagtca
     4441 ttgatccgcg ttttgttacc caaatggaat tt
//
LOCUS       DASTJP010000442         6405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1164345, whole genome shotgun sequence.
ACCESSION   DASTJP010000442 DASTJP010000000
VERSION     DASTJP010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164345"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /gene="tuf"
                     /locus_tag="VFQ23_12735"
     CDS             <1..142
                     /gene="tuf"
                     /locus_tag="VFQ23_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET9907510.1"
                     /translation="EMVMPGDNVNLTVELIVPVALEQGSKFAIREGGLTVGAGVVTKI
                     LE"
     gene            163..333
                     /gene="rpmG"
                     /locus_tag="VFQ23_12740"
     CDS             163..333
                     /gene="rpmG"
                     /locus_tag="VFQ23_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET9907511.1"
                     /translation="MASKKKDVRPVITLQCNDCKERNYTTEKNRRNDPNRLEFSKFCP
                     RCRKHTTHRETK"
     gene            398..470
                     /locus_tag="VFQ23_12745"
     tRNA            398..470
                     /locus_tag="VFQ23_12745"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:431..433,aa:Trp,seq:cca)
     gene            544..756
                     /gene="secE"
                     /locus_tag="VFQ23_12750"
     CDS             544..756
                     /gene="secE"
                     /locus_tag="VFQ23_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET9907512.1"
                     /translation="MAVKDKGARPNAFQAFFRETAGELRKVSWPTWPEARQLTGLVLI
                     VMIIMGIILGLTDGGARALLNLLLGF"
     gene            1075..1578
                     /gene="nusG"
                     /locus_tag="VFQ23_12755"
     CDS             1075..1578
                     /gene="nusG"
                     /locus_tag="VFQ23_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET9907513.1"
                     /translation="MHCYSGYENKVRHNLEQRIETMGMKDKIFDVVIPTQEEIEVKDG
                     KRRTVERHIFPGYVLVNMLLSEESWYVVRNTPGVTGFVGMGNNPTPLRPEEVAQIVKR
                     MEAEAPTVKVTFKVGERVRIVDGPFNDFRGLVSEIDMERTKVRVMVSFFGRETPVELD
                     FLQVEKA"
     gene            1686..2111
                     /gene="rplK"
                     /locus_tag="VFQ23_12760"
     CDS             1686..2111
                     /gene="rplK"
                     /locus_tag="VFQ23_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET9907514.1"
                     /translation="MAKKFKAIVRLQLEAGKANPAPPVGPALAGHGINIMAFCKEYNA
                     RTSNRPGEVLPAEITVYTDGSFTFILKTPPAAVLLRKAAKVAKGSGVPNKEKVGRVTR
                     AQVKEIAEIKMKDLNAINLEGAIKQVEGTARSMGINIVG"
     gene            2183..2887
                     /gene="rplA"
                     /locus_tag="VFQ23_12765"
     CDS             2183..2887
                     /gene="rplA"
                     /locus_tag="VFQ23_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET9907515.1"
                     /translation="MAKHGKKYNAALAKVDLEKEYSVRDAVALAKETSTTKFDSTVEV
                     HMRTALDPRQADQQVRDVVVLPHGLGKTVRVLVFAQGEGAATARSSGADLVADDDETL
                     KKIEGGMLDFDVAIATPDAMGRVGRLGRVLGPRGLMPNPKAGTVVNAADLPRAIKEAK
                     AGRVEFRLDKTANIHVSVGKVSFTTDQLSDNFTALMDAVHKARPAGAKGEYIKRITLT
                     TTMGPGIKVSPGDTHS"
     gene            3129..3668
                     /gene="rplJ"
                     /locus_tag="VFQ23_12770"
     CDS             3129..3668
                     /gene="rplJ"
                     /locus_tag="VFQ23_12770"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET9907516.1"
                     /translation="MAITKERKEEVLATYADWMKRSQAVILVEYTGAKMKDLDAIRAK
                     VRESGGEFHILKNTLARRAFANSGMEFPQDYLVKSTAVSFAFTDPASTAKALNEATKG
                     KEFVKVKGGFMSGQSLNAAQVKALADMPPLPVVRAQLLGMLQAPAGKLVRTIAEPARG
                     LAAVVKAFAEKTSEVSAAA"
     gene            3732..4115
                     /gene="rplL"
                     /locus_tag="VFQ23_12775"
     CDS             3732..4115
                     /gene="rplL"
                     /locus_tag="VFQ23_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET9907517.1"
                     /translation="MAGNLDQIVEELSTLSVLEAAELVKKLEEKWGVSAAAPVAMAAA
                     PGGAAAAAEPVEEKTEFDVVIKETGAKKIDVIKVIRQLTNLGLGEAKTMAETAGSKVL
                     TGAGKDAANEAKKKLEEAGATVVLE"
     gene            complement(4184..4492)
                     /locus_tag="VFQ23_12780"
     CDS             complement(4184..4492)
                     /locus_tag="VFQ23_12780"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9907518.1"
                     /translation="MYGTIARFRIKPGVKDDFIKAMDDLGGATIDGWVADYYFQMNKD
                     PDEFYLVAIFKDRGTYDANADSAEQHERYLVFRSFLEDDPEWHDGFILSGTGPGSVVK
                     "
     gene            complement(4601..5284)
                     /gene="lexA"
                     /locus_tag="VFQ23_12785"
     CDS             complement(4601..5284)
                     /gene="lexA"
                     /locus_tag="VFQ23_12785"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET9907519.1"
                     /translation="MMVRKSKGLGERHQRILDFLSEYQRENRYPPSIREIGNKTGITS
                     TSVVNYYLDQLEKKGMIERDRKISRGVRLSGVNGSADTLRVPILGPIAAGLPLPELDP
                     NISYMNDNEANAVDIARSLLPSKEKGDKLFALEVQGNSMIDAMINDGDIVVMKPASDA
                     RNGEMVAVWLPRDNEATLKYFFKEKDRYRLQPANPTMKPIFVKKSEPLEIKGKVVIVI
                     RRLDRPLAA"
     gene            complement(5400..>6405)
                     /gene="corA"
                     /locus_tag="VFQ23_12790"
     CDS             complement(5400..>6405)
                     /gene="corA"
                     /locus_tag="VFQ23_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET9907520.1"
                     /translation="GGRMIRSLYYLPGKPIRKDVPPQEFPELIRDRRALLWVDFVSEP
                     PETCLPILESFGFHPLAIDDALQETHVPKLDDWGDYLYIALNYMNVSHQQHPNHSWDT
                     DVDELDIFLGKNYIVTHHDHPITAIENTWASCDRDQRTIQNGADHILYKITDNLVAGY
                     MPAVEKIDEAIDQIEDQVFDRPTPRTLERLFELKRVLLALRRIVLPQREVLNKLARDD
                     YKVIDPKDRVFFRDIYDHLVRLHDLNESLRDLVGGAQDTYLSVVNNRMNEIMKTLTII
                     TVLFMPLTFLTGYFGMNFFEPLGNLRSWTTDAVFYLTLAIIIMMPLGMYVWMRRRTWI
                     "
BASE COUNT         1680 a         1516 c         1659 g         1550 t
ORIGIN      
        1 ggagatggtg atgccgggtg acaatgtgaa cctgaccgtc gagttgatcg tgcccgtcgc
       61 tctcgagcag ggatccaagt tcgccatccg tgaaggtggt ctcaccgtcg gcgcgggtgt
      121 cgttaccaaa atattggaat aagagaaagt tggtacgtaa gaatggcttc taagaaaaaa
      181 gacgttcgac cagtcatcac gctgcaatgc aatgactgca aggaacgaaa ttacacgacc
      241 gagaaaaatc ggcgaaatga cccgaaccgt cttgagttca gcaagttttg cccgcgctgc
      301 agaaaacaca cgacgcatcg cgaaaccaaa tagatttgta gttaggtagt cgcttagtta
      361 gcaaattacc aaaacctgta aactacctaa cccatttagg ccagtagctc aattggtaga
      421 gcactcgtct ccaaaacgag cggttgtggg ttcaagtcct gcctggcctg taaaaaataa
      481 atggcaacgc cgctgtgtat gtagggcggc gttttagccg taagaatgac caaggagtgg
      541 attgtggctg taaaagataa aggagcaaga ccgaacgcat ttcaagcgtt cttccgtgaa
      601 actgctggtg aactccgcaa agtttcctgg cccacctggc ccgaagcgcg gcagttgaca
      661 gggcttgttt taatcgtgat gatcatcatg ggcattatcc tgggcttgac cgatggtggt
      721 gctcgcgcac ttttgaattt gctgctcgga ttctaaagaa aggaaaagaa agtcctcatg
      781 tcagtgcagg aaccgcaaga tcagtttgaa cgcgaaacag cggaagcaac tgaggaagaa
      841 ttgcagaatc cttcagtcga atccactgta tctgattcgg atgcatttga atcatcaagt
      901 gaatccgaga ctgttgacgt tcctatgact caggatgaat tcgaagaatc atccgatgat
      961 tccgattcgg ttgaagagtt ggatgaagta cctgctcctg cctccaaaag cgactcaccg
     1021 gcttcggatg aactccctca ccccgaagcg gcagagggac ctgcctggta tgtaatccat
     1081 tgttattcgg gctacgagaa taaagtacgt cataacctgg agcagcgcat cgaaacgatg
     1141 ggcatgaaag acaagatctt cgacgtagtc attccgactc aggaagagat cgaagtcaag
     1201 gatggcaagc gtcggacggt ggaacgacat atcttccccg gctacgtgct tgtcaacatg
     1261 ctcttatccg aagagtcatg gtatgtagtc cgcaatacac ctggcgtcac tggcttcgta
     1321 ggcatgggaa ataaccccac tcccctgcga cctgaagaag ttgcccagat cgtcaagcgc
     1381 atggaagccg aagcgccgac tgtcaaggtc acgtttaaag ttggtgaacg agtccgcatt
     1441 gtggatgggc cgttcaatga cttccgcggt cttgtttcag agatcgatat ggagcgcacc
     1501 aaggtccgag tcatggtttc gttttttgga cgcgaaacgc ctgtagaatt ggatttctta
     1561 caggtagaaa aagcgtaaat aaaatcatta caggctgcaa cttgcgcgaa gcgaaatcct
     1621 actctgcagc ctgaaacagt gggagtggcg caaaaacgcc acgctaaaac caccaaggag
     1681 taaaaatggc aaagaagttt aaggcaatcg ttcgtctcca gcttgaagct ggaaaagcca
     1741 accccgcccc tcccgtggga cctgcgttgg ctggtcatgg tatcaatatc atggcctttt
     1801 gcaaggaata caatgcccgc acctcgaacc gaccgggtga agtgcttccg gcagagatca
     1861 ctgtgtacac agatgggtca ttcacattca ttttgaagac ccctccggct gcagtgctgc
     1921 tgcgcaaagc cgcaaaggtc gcaaaaggtt caggcgtgcc gaataaggaa aaggtcggcc
     1981 gggttactcg ggctcaagtg aaagagatcg cagaaatcaa gatgaaagat ttgaatgcga
     2041 tcaacctcga aggcgcgatc aagcaggttg aaggtacagc ccgctcaatg ggaattaata
     2101 tcgtaggata attttgtggg agggtcatgg agagacggta actgccgtct gctacgggcc
     2161 tgtttgcacc acggaggata aaatggctaa acacggaaaa aaatataacg ccgctctggc
     2221 gaaggtggat ttggaaaagg aatactccgt acgcgatgca gtcgcactcg ctaaggaaac
     2281 tagtaccacc aaattcgatt caactgtaga agttcatatg cgtactgcgc tggacccacg
     2341 tcaggctgac cagcaggtgc gggatgtcgt cgtattgccg cacggattgg gtaaaactgt
     2401 ccgtgtgctg gtatttgcac agggtgaagg agcagcgact gctcgttctt ccggcgctga
     2461 tctcgttgct gacgatgacg aaaccttgaa gaagatcgaa ggcggcatgc tcgattttga
     2521 tgtcgctatc gcaacgccgg atgcgatggg acgcgtgggt cgactcggac gagtgctcgg
     2581 tccgcgcggg ctgatgccga acccaaaagc cgggacagtt gtcaatgccg cggatctgcc
     2641 gcgcgccatc aaagaagcga aggccggtcg tgttgagttc cgtctggata agacggctaa
     2701 cattcatgtt tcggtaggca aagtttcttt cactacagac cagctttcag ataatttcac
     2761 tgcgttgatg gatgctgttc acaaagcgcg tcctgcaggc gccaagggtg aatacatcaa
     2821 acgtatcact cttacaacca cgatgggtcc cgggatcaaa gtaagccccg gagatacaca
     2881 ttcttgaggt gaccgggtat aatatcgccc gataaactaa atagccactt cgccaatgaa
     2941 ggcgggtgtc ccgagtttag gtctcgatac gtgctttgca ctactcgacc tccctccggg
     3001 acttaatttc ctgctcaggt gaggacccaa agcaatctcc ccctcctatt aggtcgggtt
     3061 ttgcctgaag tctttgcctc gcgaaacgag tggcaaagac ttttttattg aaaggaggtg
     3121 agtgtccctt ggcaattaca aaggaaagaa aagaagaagt gctggctacc tatgcagact
     3181 ggatgaagcg tagtcaggct gtgattctgg tggagtacac tggtgcaaaa atgaaagatc
     3241 tggatgccat ccgcgcgaag gtccgtgaat ctggcggtga attccacatc ttgaagaaca
     3301 ctttggcgcg tcgtgctttt gcaaacagtg gaatggaatt cccacaggat tatcttgtga
     3361 aaagtaccgc cgtttcgttt gcttttaccg atccggcttc cactgcaaaa gcactaaacg
     3421 aagctacaaa aggcaaagaa ttcgtcaaag tcaaaggcgg attcatgagt gggcagagtt
     3481 tgaatgccgc tcaagtcaaa gccctggctg atatgccgcc gctgccggtt gtccgcgcac
     3541 aattgcttgg catgttgcag gctcccgctg gcaaactggt ccgtaccatt gctgaacccg
     3601 ctcgcggctt ggctgctgtt gttaaagctt tcgcagagaa aacgagcgaa gtaagcgccg
     3661 cggcgtaatc ggcatgcgca ttacgcaatc aaaaataaaa attatttagg ttagtaagga
     3721 gtaactatac aatggctgga aatctcgatc aaatcgtgga agaactgtcc acactgtccg
     3781 ttcttgaggc ggctgagctc gtgaagaaat tggaagaaaa gtggggcgtc tctgctgccg
     3841 cgcctgtggc aatggctgcc gctcccggtg gcgctgctgc ggcagctgaa cccgtcgaag
     3901 agaagacaga gttcgatgtg gtcatcaagg aaactggcgc caagaaaatt gacgtcatca
     3961 aggtcatccg tcaattgacc aatctgggtc tcggcgaagc caaaacaatg gctgagaccg
     4021 ccggttccaa ggtacttacg ggtgctggta aggatgccgc gaacgaagcc aagaagaaac
     4081 ttgaagaagc tggcgcgaca gttgttctcg agtaagattt gcgcttagaa actgacaaaa
     4141 gacggctcga ttgagccgtc ttttttattg aaatcctgca attttatttt acgacggagc
     4201 ctggacctgt cccagacaaa atgaacccgt cgtgccattc tggatcatcc tctaagaacg
     4261 atcgaaagac aagatagcgt tcatgctgtt cggcgctgtc tgcattggca tcgtacgtcc
     4321 ctctatcctt gaagatcgcg accagataaa actcatcagg gtccttgttc atctgaaaat
     4381 aataatccgc cacccagcca tcaatggtcg cgccgcccaa gtcgtccata gctttgatga
     4441 agtcatcctt tacgccaggt ttgatacgaa agcgcgcaat ggttccgtac attagacact
     4501 cctttttgat caaattataa gccttgatgt tcatcgggtg aatcaaaaga gacgaccttt
     4561 tggtcgtctc tttgtttctt ctttacttcg ccttactatg ttatgcagcg agcggtcgat
     4621 ccaatcttcg gataacgatc actactttac ctttgatctc cagcggttca ctctttttga
     4681 caaagatagg cttcatcgtt gggttggcag gctgaaggcg ataacgatct ttttccttga
     4741 aaaagtactt cagggtcgct tcgttatcac gtggtagcca gactgctacc atttctccgt
     4801 tgcgtgcatc ggaagctggt ttcatgacaa cgatatctcc atcgttgatc atggcatcga
     4861 tcattgaatt tccctgtact tcgagtgcga agagtttatc gcctttttct ttcgagggca
     4921 ggaggctgcg tgcgatatca actgcatttg cctcgttgtc gttcatataa cttatattcg
     4981 gatcaagctc ggggagggga agaccagcag caatcggtcc aaggataggt acgcgtaagg
     5041 tatcagcaga tccgtttacg cctgaaagtc ggacaccgcg cgaaatcttg cggtcgcgct
     5101 cgatcattcc tttcttttca agctgatcga gatagtaatt cacaactgag gtggatgtga
     5161 tcccggtctt attgccgatc tcccgaatgg aaggtggata ccggttctca cgttggtatt
     5221 ccgaaaggaa atccaaaatc ctttggtgtc gttcgcccaa acctttactt ttgcgtacca
     5281 tcataatagc tccttctgcc agaaatttct gacatgcatg agcattattc gctcatttgt
     5341 gctatgaatg ataaccgaac gtctgttctg tgtcaagtgc gcgaggaatg agtttttgcc
     5401 taaatccagg ttcggcgccg catccaaaca tacataccaa gcggcatcat aattatgatg
     5461 gccaatgtca gataaaacac cgcatccgtc gtccaacttc tcaagttccc gaggggttcg
     5521 aagaagttca ttccgaaata tccggtcaga aaggtgagcg gcatgaatag aacggtgata
     5581 attgttaacg tcttcatgat ttcgttcatg cgattgttga ctacagataa atacgtatcc
     5641 tgcgctccac cgaccaggtc gcgcaggctt tcgttgagat catgcaaacg caccaggtga
     5701 tcgtagatat cacgaaagaa cacgcggtct tttgggtcga tcactttgta atcgtcacgc
     5761 gcaagcttgt tcaacacttc acgctgtggg agaacgatcc gcctgagtgc cagtaaaacg
     5821 cgctttagct cgaacagccg ctccaacgtc cgaggggtgg gccgatcgaa gacctgatct
     5881 tcaatctggt caatggcctc gtcaattttt tccacggccg gcatataccc cgcgaccaga
     5941 ttatcggtga tcttataaag aatgtgatcg gcgccgttct gaatcgtacg ttgatctcgg
     6001 tcgcaggaag cccacgtgtt ctcaatagca gtaatcggat gatcgtgatg agtaactata
     6061 tagttttttc caagaaagat atcaagttca tccacgtcag tatcccagct atggttagga
     6121 tgttgctgat gcgagacatt catatagttg agggcaatgt aaagataatc tccccagtca
     6181 tccagttttg gaacatgagt ttcctgaagg gcatcgtcaa tcgctagagg atgaaaaccg
     6241 aatgattcca ggatgggtaa gcaggtttcg gggggctcgc ttacaaaatc cacccataaa
     6301 agcgcccggc gatcacggat cagttcaggg aattcctgag gaggaacatc ctttcgaatg
     6361 ggtttgccgg gtaaatagta taacgaacgg atcatacgtc ctcca
//
LOCUS       DASTJP010000443         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1412787, whole genome shotgun sequence.
ACCESSION   DASTJP010000443 DASTJP010000000
VERSION     DASTJP010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412787"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFQ23_12795"
     CDS             complement(<1..560)
                     /locus_tag="VFQ23_12795"
                     /inference="COORDINATES: protein motif:HMM:NF039255.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HET9907521.1"
                     /translation="MYHPTTRVLAVLALLQAHGRMSGAELAERLEVNIRTLRRYIIML
                     QDLGIPIEAERGRNGAYVLSAGFKLPPMMFTNEEALALTVGLISARRLNLADTDRAVE
                     SAFAKLERVMPLDLKSRVQALTETITLDQKAASSAAPSEVVLSEMSSAAQLQQRVHIQ
                     YRSISGEDSERDFDAYGLTYYLQKWYV"
     gene            complement(585..1328)
                     /locus_tag="VFQ23_12800"
     CDS             complement(585..1328)
                     /locus_tag="VFQ23_12800"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HET9907522.1"
                     /translation="MSKPTYSPIVDLRMYTHQPNKRDDFIKLFEDEFIETQEAVGIQV
                     IGQFYDRDDPDRCIWLRGFNDMPAREQSLTAFYNGPIWKAHREATNATLVDSDNVLLL
                     RLVHPTSGFSLNRHRPPFGSRATQDGFVTATIYYFNRYVDSDFIHYFENTIHPLLMDT
                     DAQLLAYFVNEDGPNTYPRLPVREGEHVFVWFAGFQSHEAYEHHNSEFARSTLWSQEI
                     SKYLKRRLMRKPEVLKLSPTPRSRLTGII"
     gene            complement(1325..1801)
                     /locus_tag="VFQ23_12805"
     CDS             complement(1325..1801)
                     /locus_tag="VFQ23_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907523.1"
                     /translation="MAEENDGRDDFDFLIGTWRVHHRRLKERLKGSTEWEEFEGETVD
                     RKILNGIGNQDENILHRKTGSIHAISLRLFNSESKEWSIYWSTNVTGTLDVPMVGGFK
                     ERTGEFYSQEIFEGQHIYNRFIWTKITANSCQWEQAFSEDGGKTWETNWIMEFERV"
     gene            complement(1805..2287)
                     /locus_tag="VFQ23_12810"
     CDS             complement(1805..2287)
                     /locus_tag="VFQ23_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907524.1"
                     /translation="MQTENDGRNDFDFLVGKWIMRHRRLKERLKGCTEWEEFEGTAVG
                     EKLLNGLGHCDEVIMNRESGPNHGYTLRLFDVNSKEWSIYWAAGASCLLDVPMIGKFK
                     DGVGEFYSQEVYEGRHIFCRFTWSKITANSAQWEQAFSIDGGKTWETNWMNTFERIED
                     "
     gene            2422..2994
                     /locus_tag="VFQ23_12815"
     CDS             2422..2994
                     /locus_tag="VFQ23_12815"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9907525.1"
                     /translation="MKPTELPIVPFENKKTLATWLAKEHEKSKGLWLKLAKKNSGIPS
                     ITYEEALDVALCYGWIDGQKRSFDNLYWLQKFTPRGLKSIWSKINTEKAERLIASGDM
                     KPAGLKAIEAAKQDGRWEAAYSSQKNSSIPEDFQAALDKNKKAKAFFTTLKSAERYSF
                     LFRIQTAKKAETRARRIEQFVEMLERNEKL"
     gene            3049..>3455
                     /locus_tag="VFQ23_12820"
     CDS             3049..>3455
                     /locus_tag="VFQ23_12820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET9907526.1"
                     /translation="MPTTIDEAEIHQIVKNVVQQALGTVERGQAAVASSSTKKIVAIG
                     ADHGGYELKETLKNDLSTLGFDVMDVGTVSKEAVDYPDFAHAVAQSVGTGKAWRGIMI
                     DGAGIGSCIVANKVPGVRAGMAYDVSSAINSRE"
BASE COUNT          889 a          858 c          820 g          888 t
ORIGIN      
        1 acataccatt tttggagata atacgtcagc ccatacgcgt caaaatctcg ttccgaatct
       61 tctcctgaaa tcgaacgata ttgaatatgc acgcgttgct gaagttgtgc tgcactactc
      121 atttcgctga gtaccacttc acttggagca gcggacgaag ccgctttctg atccagggtg
      181 atcgtctcgg tgagtgcctg gacacggctt tttaaatcaa gtggcatcac cctttccagc
      241 ttggcaaagg cgctttcaac cgcgcggtcg gtatccgcga gattaagtcg ccgcgcggaa
      301 attaatccga cagttagtgc cagagcctcc tcgtttgtga acatcatcgg agggagttta
      361 aaacccgccg aaagcacata cgcgccattc cgaccacgtt ccgcttcgat gggaatgccg
      421 agatcctgca gcatgatgat atatcgccgc agggtgcgga tgttcacttc gagccgctct
      481 gccagctctg cgccggacat gcggccatgg gcctgtaaca aagccaatac cgccagcacg
      541 cgcgtcgtgg ggtgatacat acgccgcgag tatacaccag ccgtttaaat gatccctgtc
      601 agcctggaac gcggggttgg agataatttc aagacttccg gttttctcat caatcggcgt
      661 ttcaggtatt tcgaaatttc ctggctccag agggttgatc gcgcaaactc gctgttgtgg
      721 tgttcatatg cttcgtgact ctgaaagccc gcaaaccaca cgaagacatg ttcgccctcc
      781 cgaacgggca accgtggata ggtatttgga ccatcctcat taacaaagta ggcgagaagt
      841 tgagcatcgg tgtccatcaa tagcggatgg attgtgtttt caaagtagtg gatgaagtca
      901 gagtccacat accggttgaa atagtaaatc gtagctgtca caaacccatc ttgcgttgcg
      961 cggctgccaa agggcggacg atgtctattg agtgagaatc ccgaagtcgg gtgaacgaga
     1021 cgcaaaagca gaacattatc tgaatcgacc aaagtagcat tggtcgcttc acgatgcgct
     1081 ttccaaattg gtccgttata aaaggcagtg agggattgtt cacgggcggg catgtcgttg
     1141 aaaccgcgca gccaaatgca tcgatctgga tcgtcccggt catagaactg tccaatgacc
     1201 tggatgccca cggcttcctg tgtttcgata aattcatctt caaagagttt gataaagtca
     1261 tcgcgcttgt ttggctgatg ggtgtacatc ctgaggtcaa cgatggggga gtaggttggt
     1321 ttgctcatac tctttcaaat tccatgatcc agttggtttc ccaggttttg ccaccatcct
     1381 cagaaaatgc ctgttcccat tggcaggagt tcgctgtaat cttagtccag atgaaccggt
     1441 tataaatatg ctgaccttca aagatttctt gtgaataaaa ttcaccggtt ctttctttga
     1501 aaccgcctac catggggacg tcgagagttc ctgtcacgtt tgtagaccaa taaatgctcc
     1561 actcttttga ctcagagttg aacaaacgta agctaatcgc gtggattgaa cctgtttttc
     1621 tgtgcaggat gttttcatcc tggttgccga tcccattcag gattttcctg tcaaccgtct
     1681 caccttcgaa ctcctcccac tctgttgaac ctttcaatcg ttccttcaat cttcgatgat
     1741 gaactctcca cgtgccgatc aggaaatcga agtcatcacg tccatcattt tcttcagcca
     1801 tgctttaatc ctctattctc tcgaaggtat tcatccagtt cgtttcccag gttttcccgc
     1861 catcaatcga aaatgcctgt tcccactgcg ccgagttcgc tgtgatcttg gaccaggtga
     1921 aacggcagaa gatatgcctg ccctcgtaaa cttcctgcga ataaaattca ccaaccccat
     1981 ctttgaactt accgatcatt ggaacatcca acaggcaact tgcgcccgct gcccaataga
     2041 tgctccattc ttttgagttc acatcgaaaa gccgtagcgt gtaaccatga ttgggacctg
     2101 actctctgtt cataatcact tcgtcacaat gacccagtcc gttcaaaagc ttttcgccga
     2161 ctgccgttcc ttcaaattcc tcccactcgg tacagccttt caggcgctct ttcaatctgc
     2221 ggtgacgcat tatccacttc ccgaccagaa agtcaaaatc atttcgtccg tcgttttctg
     2281 tttgcatgct ctgctcctaa tacgatatgt tgtacatatt cagtatagga agtaatcagg
     2341 acaggattgt tcctaattta ggattagaaa acggctctct ttccccgacc ttttgcctag
     2401 tgttgtaaaa ttcataaact tatgaaaccg actgaacttc ctatcgtgcc gtttgagaac
     2461 aaaaagacat tggcaacctg gcttgccaaa gagcacgaaa aatcgaaggg gttgtggctg
     2521 aagcttgcca agaagaactc aggaatacct tcaatcacgt acgaggaagc actggatgtt
     2581 gctctatgct atggctggat cgatggtcag aagcggagtt ttgacaatct atattggctt
     2641 cagaaattca cgccccgcgg attaaaaagt atttggtcga agatcaacac cgaaaaggca
     2701 gaaagactca ttgccagcgg cgatatgaag cccgccggac tcaaggctat tgaagccgca
     2761 aaacaggacg gtcgctggga ggcggcgtat tcctcgcaga aaaacagttc cattcccgaa
     2821 gattttcaag ccgcgttgga taagaacaag aaggcaaaag ctttctttac cacattgaag
     2881 agcgcggaaa ggtattcgtt tttattcaga attcaaaccg cgaaaaaagc ggagactcgt
     2941 gcaagacgaa tcgagcagtt tgtggaaatg ctggagcgaa atgagaagct ataaataata
     3001 gccctccact tgacgcaatc acttttcacg aagataatcg aaccaatcat gcccaccact
     3061 atagacgaag cggagatcca tcaaattgta aagaatgtcg ttcagcaagc attggggacc
     3121 gttgaacgcg ggcaggctgc ggtagcatcc tcttctacaa agaagatcgt tgccatcggc
     3181 gccgatcacg gtggttatga gttaaaagaa accctcaaaa acgatttgag cacactgggg
     3241 tttgatgtaa tggatgtagg tacggtcagc aaagaagctg tggattatcc cgattttgct
     3301 cacgcggttg cgcagtctgt aggcactggc aaagcctggc gcggcatcat gattgatggg
     3361 gcagggatcg gtagctgcat tgtcgcgaac aaagtccctg gagtgcgcgc gggcatggca
     3421 tatgatgttt catccgccat caacagccgc gaaca
//
LOCUS       DASTJP010000444         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1226740, whole genome shotgun sequence.
ACCESSION   DASTJP010000444 DASTJP010000000
VERSION     DASTJP010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226740"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VFQ23_12825"
     CDS             complement(<1..1568)
                     /locus_tag="VFQ23_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9907527.1"
                     /translation="MMQTIGQKLKAAREEKRLTLEKVFEDIRIRIPYLQALEADDLSI
                     MPSPVQARGYLRNYAQYLDLNFDQLLNELRAETQTTAEIIGPAEDASTLRPSLRLEES
                     ISPTSQPESQIINSAEAPQIEDAVEETPLPNPPKPARRKKKDSPPQSLEVAADNPDTV
                     QLPKRRGRKKVVPDPEPTIPIVESVPEQIEEPVTSTDSVQDVAEVPIEEVQPELLQEE
                     PKEDINDNLWQSWMNRLGSVLSARSRRGTLVRRDTTIEEIDKESIAVVPEISREEATQ
                     FDHSEIKNNASVDWAEIFREIGRELRQRRELLSLHLEEVERNTHVKAHYLDALEKGAI
                     DQLPSTVQTRGMLSNYATFLDLDVDALLLRFADALQAWHRERNPQKPARKPGQPIIAP
                     IPSIRSFIAGDMIFGIGIAILLVGFSIWGVSRVLTIQSQQEVQPTAPSISDVLLASPE
                     ASEFTATSEALPAQIPGEATVTIVIPTQNLDVNVQVNLVAVERTYMRVIVDGEEVFNG
                     RVVPGTAYPYQAET"
     gene            complement(1669..2196)
                     /gene="rplI"
                     /locus_tag="VFQ23_12830"
     CDS             complement(1669..2196)
                     /gene="rplI"
                     /locus_tag="VFQ23_12830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9907528.1"
                     /translation="MKVLLLKDVYKLGRAGDIKKVADGYGRNFLLPQKLAVLATAGAL
                     KQIEKIRSQAEVRRTEQNSELKDLATQINGVTLVFSAKAGETGKLYGSITTQDVATAL
                     QEKVRYEVKKQQIDMQPIRNLGEFSAFVRLTMDLVPEVKIIVHREGEAIEEGESTPSE
                     TPVEEPAPAAEQAEA"
     gene            complement(2314..>2813)
                     /locus_tag="VFQ23_12835"
     CDS             complement(2314..>2813)
                     /locus_tag="VFQ23_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HET9907529.1"
                     /translation="RVREFDHLRQRAEDLSDSPVQVIHQEDTFFNTEKGRLKLRVLAP
                     DRAQLIYYLRSDQEGPKRSNYHIFETPDPENLKRVLELAYGIRGVVKKTRYLYMAGQT
                     RIHLDEVDGLGHFMELEVVMQPGQSDADGQEIAEKLRVKLGVEKADLLEGAYMDLLES
                     TEQSE"
BASE COUNT          627 a          713 c          711 g          762 t
ORIGIN      
        1 tgggtctcag cctgatatgg ataggcagta ccgggcacca ctcggccatt gaaaacctct
       61 tctccatcta caattacacg catataggtg cgttccaccg caaccagatt gacctggaca
      121 ttcacatcca ggttctgagt cgggatgacg atcgtcacag tagcctctcc gggaatttgc
      181 gcgggcaacg cttcggaggt ggctgtaaat tcggacgcct cgggggatgc cagcaacacg
      241 tctgaaatgg acggggcggt tggttgtacc tcttgctggc tttgaattgt taatacgcgg
      301 ctcactcccc agattgaaaa tcccactaac aggatggcga tgccgatgcc aaaaatcata
      361 tcgcctgcga taaaacttcg tatcgaggga atgggtgcga taatgggctg accaggcttg
      421 cgcgcaggtt tttgtgggtt tctctcacga tgccaggctt gcagcgcgtc tgcaaagcgc
      481 agcagcaagg cgtcaacatc caggtcgagg aaggtcgcgt agtttgacaa cataccgcgc
      541 gtttgcacgg tcgatggcag ttgatcgatc gcaccttttt caagggcatc cagataatgc
      601 gccttgacat gtgtattacg ttctacttct tcaaggtgca ggcttaacag ttcccggcgc
      661 tggcgaagtt cacggccgat ttccctgaaa atttcagccc aatcgacaga tgcattattc
      721 tttatttcgc tatggtcaaa ctgtgttgct tcttcccgtg atatttctgg cacgactgcg
      781 attgattctt tatcaatctc ctcgatcgtt gtgtccctcc gaaccaaagt accgcgtctg
      841 gaacgagcgg acaagacaga tcctaatcga ttcatccacg attgccagag attatcgttg
      901 atatcttctt taggttcttc ctgcaggagc tcgggttgca cttcttcgat tggaacttca
      961 gccacgtctt gaacagagtc tgttgaagta acaggctctt caatctgctc tggaactgat
     1021 tccacaatgg ggatggttgg ctcaggatcc gggacaactt tctttcgacc acgtcgcttg
     1081 ggaagttgaa cggtgtccgg gttgtcggcg gcaacctcca gcgattgagg cggcgagtcc
     1141 tttttcttgc ggcgagccgg cttaggcgga ttaggaagcg gcgtttcctc tactgcatct
     1201 tcgatttgcg gcgcctccgc ggaattaatt atttgtgact ccggttggct ggtgggcgaa
     1261 atactctctt ccaaccgaag ggagggccga agcgtcgacg catcctctgc agggccaatg
     1321 atttcggcag ttgtctgagt ctccgccctc agttcgttta acagctgatc aaagttaagg
     1381 tccagatatt gagcatagtt ccgcaaataa ccccgcgctt gaaccgggga aggcatgatc
     1441 gaaagatcat ctgcttccag cgcctgtaga tatggaattc gaatacgaat gtcttcgaag
     1501 accttttcaa gggtaaggcg cttttcttcg cgggcggctt tgagcttctg gccgatagtt
     1561 tgcatcatgc tacacgtagc aattctgtgg ataggcggat aaacgaaaca tcaccagttc
     1621 cccatctatc gggaatgaac tggtgatgtc atgggcggat aggtttgact atgcttcggc
     1681 ttgttcagcc gctggagcag gttcttcaac cggagtttca gaaggcgttg actcgccttc
     1741 ttcaatcgct tcaccttccc ggtgtacgat gattttgacc tcggggacaa ggtccatagt
     1801 gagccgaaca aaagccgaaa actctccaag attgcggatc ggctgcatat cgatctgctg
     1861 tttcttgact tcgtaacgga ctttctcttg aagagcagtt gctacatctt gtgtggtgat
     1921 ggagccatag agtttgcccg tttcgccggc cttggcggaa aagactaaag taactccgtt
     1981 gatttgcgtt gccagatctt taagctcaga gttctgttcc gtgcgacgga cttcagcctg
     2041 cgagcggatt ttctcgatct gtttgagagc gccagcggtg gctagcacag ccagtttctg
     2101 cggaagcaaa aagttgcggc cgtagccatc ggcaactttt ttgatgtcgc ccgcccggcc
     2161 cagtttgtaa acgtccttga gaagcaatac tttcattgtt taagcccttt ctttcaaaat
     2221 ggtgattttt ttcgagcgaa gggtactacg ctcggcggcg tattttacca caggttccat
     2281 gaagtcacac cgactttggc caaacttctt caattattca gattgttccg ttgattccaa
     2341 caaatccatg tatgcaccct cgagcaggtc agccttttct accccaagtt tgacccttaa
     2401 tttttcggca atttcctgcc catccgcgtc gctttgccca ggctgcatca caacttccaa
     2461 ttccatgaaa tgacccaacc catccacctc gtccaaatga atgcgagttt gcccagccat
     2521 atacaggtaa cgagttttct taaccacccc gcgaattcca taggcaagct ccaacacgcg
     2581 tttgagattc tcagggtctg gtgtttcaaa aatatgataa ttcgaccgtt ttggaccttc
     2641 ctggtcagag cgtaaataat agattaattg tgcgcggtca ggcgcaagca ctcgaagttt
     2701 caaacgtcct ttttctgtgt tgaaaaaagt gtcttcctga tggatgacct gtacggggga
     2761 atcgcttaag tcctcagcac gctggcggag atggtcaaac tcgcggacac gtg
//
LOCUS       DASTJP010000445        10312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_83699, whole genome shotgun sequence.
ACCESSION   DASTJP010000445 DASTJP010000000
VERSION     DASTJP010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83699"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFQ23_12840"
     CDS             complement(<1..504)
                     /locus_tag="VFQ23_12840"
                     /inference="COORDINATES: protein motif:HMM:NF018008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9907530.1"
                     /translation="MMPRQLFTAEDIRRLARDGAETLLLAPDDIVTHEAVDVAVALGL
                     KLIRETESSSSLRRETPPTASNLPPLKVVKMSGVQVEPFLEGKTTPGTNVWLKDVVVT
                     QDRSPMGAGYMSLDKGEMQWTLTYDEIDIVLEGELVITRGNEQVRGKAGDVIYIPKGS
                     SITFGTPN"
     gene            complement(654..986)
                     /locus_tag="VFQ23_12845"
     CDS             complement(654..986)
                     /locus_tag="VFQ23_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HET9907531.1"
                     /translation="MPVDVSMIALGMVETKGLIGAIEAADAMVKAANVTLIGSEYVGG
                     GYVTVMVRGDVGAVKAATDAGAAAAKRVGELASVHVIPRPHSDVELILPQNSRGSFGG
                     RAGGGKEK"
     gene            complement(1212..2723)
                     /locus_tag="VFQ23_12850"
     CDS             complement(1212..2723)
                     /locus_tag="VFQ23_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9907532.1"
                     /translation="MTDLDNDLLSIQEARTLATQARDAQRKFLHATQAEVDRICAAMA
                     QAAADAAVQLGKMASEETGYGVPEHKTLKNLLSSQLLWEAIKDIPTVGVIRSDPARKT
                     YDIAWPMGVVAALTPSTNPTSTTMFKILISVKARNGIVVAPHPFAAKCCVETARIMAE
                     AGERAGMPKGLVNCMSRITLPGTQELMKHKYVALILATGGSDMVRAAHSVGKPAYGVG
                     PGNVPVYVDRSADVPRAAKYIVASKAFDHSVICATEQAVVADRPIAAQLEQLMKAEGA
                     YFVDDNIKQILAKNLFVGHLPNPKGVGKSPQQLAQQYGFSVPDWARILVVRLNSVGRD
                     EPLSGEKLTTVLGWYEVDGWEAGCERSIEMINYGGRGHSLVIHSRDENVIMQFGLEKP
                     VFRIVVNTMGTLGTTGFTTGVMPSMTLGSGGVGGAITGDNITVHHMYNVKKLAYETRV
                     APEAALSPGSTDDPAQRRLFTGGAPVSTAATLSSASNAQVEEIVRKVLLELKK"
     gene            complement(2882..3538)
                     /gene="eutL"
                     /locus_tag="VFQ23_12855"
     CDS             complement(2882..3538)
                     /gene="eutL"
                     /locus_tag="VFQ23_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HET9907533.1"
                     /translation="MPILDPLYGTPLAVQLIPQVDRNFAEHLKLRDDQRSIGLLSVDN
                     DDATYVAIDEATKMANVEVVYARSFYAGAKHASGKWSGEIMAIIAGPDPAEVRAGLSA
                     AVDYIKTQAIWYSANEDDSIAFFPHVVSRTGTYLSQVCNIPVGTPIAYLVATPNEGLV
                     ALDAALKAADVSIVALTMPPSETNYMGVMLTGDQPACKAAAVAFQNKVLEVAINPINY
                     "
     gene            complement(3548..4504)
                     /gene="eutC"
                     /locus_tag="VFQ23_12860"
     CDS             complement(3548..4504)
                     /gene="eutC"
                     /locus_tag="VFQ23_12860"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HET9907534.1"
                     /translation="MDDTQLAAIVDAIVRELKESGAVKPGASSSDNSPSPVSTTLSSA
                     SVVPVKPRATVTGGDLQIDLPDPATSEARNTPRVKNPKDAQGLRALMASTTARIGVGR
                     AGPRYSTASLLLFQGDHAVTQDALYRDVDQRLLDEFNLFTVQTRITGGKQEYLLRPDL
                     GRLLNDDAKRTINEKCQKNVNVQLVVGDGLSAAAVEANLRQMFPVIKQGVQTAGLTLG
                     TPFFIKYARVGVMNDVGELIKPDVVILLIGERPGLGRAESMSAYMGYKPKYGDTDADR
                     DVVCNIFENGGTNPLEAGAFVVQIAQKMRKHQASGVKLKLAK"
     gene            complement(4507..5874)
                     /locus_tag="VFQ23_12865"
     CDS             complement(4507..5874)
                     /locus_tag="VFQ23_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HET9907535.1"
                     /translation="MLLRTKLHGKTYEFPDIRLLMGKANEEKSGDRLAGVGAETAAER
                     VAAKLVLAEVPLWVLHDNPAVPYEEDEITRVIMDAVDQSIYNEIKGWTVGEFREWILA
                     DTTSTDMIRRVSNGLTAEMIAGVTKLMSNLDLMYAAKKIPVSAHCNNTIGAPGTLSSR
                     NQPNHPTDSPEGIRAEIYEGLSYGSGDSVIGINPVDDSYGSVARLLDMTYDVIKTWNI
                     PTQNCLLSHVTTQMKCMQSGSPVGLVFQSLAGSQKGNDSFGISVGMIDEADEMAKKYC
                     FPKGPNYMYFETGQGSELSADAHNGWDQLVLEARCYGFAKRYSPYQVNTVVGFIGPEY
                     LYDARQIQRAGLEDHFMGKLTGIPMGCDACYTNHARADQNAIENLAVMLTAAGCNYFM
                     GVPMGDDSMLSYQCTSFHDAPTLRQLFKLRPAPEFEKWMVDLGLMKDGVLTEKAGDPT
                     IFLKR"
     gene            complement(5903..6538)
                     /locus_tag="VFQ23_12870"
     CDS             complement(5903..6538)
                     /locus_tag="VFQ23_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET9907536.1"
                     /translation="MFESSQLYLFLIASFALLITPGPAVLYIVARSMNQGKMAGIASV
                     LGVETANFLHASAAALGLSAILLSSALAFNLVKYLGAGYLIYLGVRKILSKGEETKAK
                     LAQQEKLSRIYSQGFIVNLFNPKTALFFFAFLPQFVNTSLTNVTLQMFLLGIMFVLMA
                     LITDSTYALVSSSLADKLNSNDNFARNQRYFAGLIYIGLGVLTAFTGSRNK"
     gene            complement(6731..7147)
                     /locus_tag="VFQ23_12875"
     CDS             complement(6731..7147)
                     /locus_tag="VFQ23_12875"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9907537.1"
                     /translation="MKISAKVENTKDNHHVALQTIDNVHSIVIPPKSTRYGSSTNGGE
                     LLFLALATCYCNDVYREAAKRNIQVDHIEVNVEGEFGAEGEPAKNVTYQAKVFSQASE
                     DEVREIMKFTDSVAEIQNTLRVLTPVKLDQIEVIKV"
     gene            complement(7282..8721)
                     /locus_tag="VFQ23_12880"
     CDS             complement(7282..8721)
                     /locus_tag="VFQ23_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HET9907538.1"
                     /translation="MSDSRALLSVGLDVGTTTTQVIFSRINLQDVARAGQIPRINITD
                     RKVIYQSPIVFTPLIDAETIDADRLNDIVRGEYASAGIDPSQVETGAVIITGETAKKK
                     NADEILRALSGLAGDFVVSVAGPNVESLISGRGAGAAEYSQKNYVTVTNIDIGGGSAN
                     SVTFQSGNPIGAAAMNYGGRILEIEHTSGRVRHIADPAKHILHDIGFTLAIGDSPSLD
                     ELRRFTDRMADMTVELVEGTSSPLAQKLYLTPPVGVSGKGTVLMFSGGIGHYFYNPIQ
                     INSVADATIHDDVGPLLAESMRKHSILNSYEIVQPSETLRATVLGAGTQTVTLSGSTI
                     WAEQEILPIKNMPVIRPALPATLEPLAISTAISEAVRRWDVNLVTDPFAIALELNRAL
                     DYTLLTQVANGLREFSSTMPADRPLVVIIERDYAQALGQTLKGLAPDRSLLVIDQVGL
                     SEGDYIDIGTPLMDGRVVPLSVKTLIFYH"
     gene            complement(8776..9066)
                     /locus_tag="VFQ23_12885"
     CDS             complement(8776..9066)
                     /locus_tag="VFQ23_12885"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET9907539.1"
                     /translation="MRIALVIGSTISTIKDEVIRGRKLLIVRNADTEGNVTGDPYIAV
                     DTVSAGAGDLVMVTDGSSARYTNQTANAPVDAVIVGVIDSLELGGKVSYRKE"
     gene            complement(9205..>10312)
                     /locus_tag="VFQ23_12890"
     CDS             complement(9205..>10312)
                     /locus_tag="VFQ23_12890"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET9907540.1"
                     /translation="LLLDSYFSAPKLKWVFENDPEIRKQAHAGKLKFGTTETWVLWSL
                     TNGKWHVTDPSTASRTLLFDMNRLEWDEELLTLFDVPRSILPEVKPSASFIGDVDFGN
                     GKPLPLQALLVDQQAALFGQACFNAGEMKCSFGTGSFLLMNIGDQPKLSQNGLVTTVG
                     WNFNGHTAYAFDGGIFVTGSAVQWLRDNLKLIPDAAASNNAALKSTDRGVVFVPALQG
                     LAAPHWRTDVRGAMFGLNRSTSSDDIVRATLDGIACRVYEVVHAMAQDAGQNPPHLKV
                     DGGPSGNPYLMQMIADLLELEVHVSAALEATAIGIANLAGVSALGTSLDELASHWKAE
                     LIYTPNMKAEERVEKLALWNKALNAAKAFHRDAG"
BASE COUNT         2429 a         2782 c         2560 g         2541 t
ORIGIN      
        1 gtttggggtg ccaaacgtga tgctcgatcc tttcgggatg tagatcacat cgccggcctt
       61 gccgcgcacc tgctcgttcc cacgggtgat caccagttcg ccttccaaca cgatatcgat
      121 ctcgtcatac gtcagtgtcc actgcatctc gcctttgtcg agtgacatgt atcccgcgcc
      181 cattggcgaa cggtcttgcg tcacgaccac atccttgagc cagacgttcg tgcccggtgt
      241 agttttgcct tcgagaaaag gctccacctg cacacccgac attttgacca ccttaagcgg
      301 aggaaggttg gatgctgtgg gcggcgtttc tcttcgtaga ctggaagatg attccgtttc
      361 ccgaatcagc ttcaaaccca gagcgaccgc cacatccact gcttcatggg tgactatgtc
      421 atccggcgcg agcaaaagag tctccgcgcc atcacgcgcg agacggcgga tatcttccgc
      481 agtgaataat tgacgcggca tcattttgcc tgtcactctg atggagcgac agcgaccgaa
      541 gaatctccat aaaacctagt agacacttcg ctacgctcac accgtcccga agcggcacct
      601 gagccgaccg cagctacggt gtgtcccttc ggaagagtga cggaagcaga atcttatttc
      661 tctttgccgc caccggcgcg tccgccgaac gagcctctcg aattttgcgg aaggatcaac
      721 tcaacatctg aatgaggtct tgggattaca tggaccgatg caagctcacc gacgcgcttt
      781 gccgcggctg caccagcatc cgtcgcagct ttgacagcgc caacgtcgcc gcgcaccatc
      841 acagtgacgt atccgccgcc aacatactca ctgccgatca gcgttacgtt tgcggctttg
      901 accatggcat cagcagcttc gatcgcaccg atgagtcctt tggtctcgac cattccaagg
      961 gcaatcattg aaacatctac gggcatggct aacttctcct ttggatcaaa ttttccgatt
     1021 agacatcgga tgtcagggtt gaaagcgttt caagacctga gatctgagtt ctaattcatc
     1081 ctctgcggtg gtcgagtagg gctgagccca tatcgagacc acaagttagt aaaatacttc
     1141 ctgaaatatg ttggtttcga tacgccctcg caacgaacgc tcggggctac tcaaccaacg
     1201 aattactcag gttatttctt caactccaac aatacctttc tcacaatttc ttccacctgc
     1261 gcattggatg cggaggataa agtagctgca gtcgatacag gggcgccgcc ggtaaacagt
     1321 cgtctttgcg cagggtcatc tgttgagccg ggagacagcg cggcctctgg agcgacgcgg
     1381 gtttcatatg ccagcttttt gacgttatac atgtgatgca cggtgatgtt atcgcccgtg
     1441 atcgcgcctc ccacgccgcc cgagccgaga gtcattgaag gcatcacgcc tgtggtaaag
     1501 ccggttgttc caagcgttcc cattgtattg accacaatgc gaaatactgg cttttccaaa
     1561 ccgaactgca taataacatt ttcatcccgc gaatgaatca ccagggaatg gccgcgaccg
     1621 ccatagttga tcatttcgat actgcgctca caacctgcct cccagccatc cacttcatac
     1681 caaccaagca ctgtggtcag cttttcgccg gaaagcggtt catcacgtcc cacgctattt
     1741 aagcggacaa caagaattcg cgcccaatcg gggacactga atccatactg ctgtgcgagc
     1801 tgctgcggac tcttgccaac ccctttcgga ttcggtaaat gaccgacgaa caaattcttc
     1861 gccaggatct gcttgatgtt atcgtcaacg aaatacgcgc cttctgcctt catcaactgc
     1921 tcaagctggg cagcaatcgg tcgatctgcc acgacagcct gttctgttgc gcaaatgacg
     1981 gagtgatcaa acgccttgga cgccacaata tacttcgcgg cgcgcggcac atctgctgat
     2041 cgatcgacat agaccggcac attccctgga ccaacgccat acgccggctt gcccacagag
     2101 tgcgcggcgc gcaccatatc tgagccgccg gtcgccagga tcaacgccac atatttatgc
     2161 ttcatcaatt cctgtgtgcc tggcaaggta atcctgctca tgcaattgac cagccccttt
     2221 ggcatcccgg ctcgttcgcc ggcttcagcc atgatgcggg cggtttctac gcagcatttc
     2281 gctgcaaaag gatgcggcgc cacgacgatg ccgtttcgcg ccttcacaga gatcaatatt
     2341 ttgaacatcg ttgtggaggt cgggtttgtg gacggagtca atgcagcgac cacgcccatc
     2401 ggccaggcga tgtcgtatgt cttcctggca gggtcactgc gtatcacacc tactgttgga
     2461 atgtctttta tcgcttccca caacaattgt gaggataaca ggttcttcaa tgtcttgtgt
     2521 tcaggaacac catatcccgt ttcttcgctc gccatttttc caagctgtac ggcggcgtcg
     2581 gcggcggcct gagccatcgc cgcgcagatg cgatccacct cagcctgggt tgcatgcaaa
     2641 aatttgcgct gtgcatcccg cgcctgggtt gccagggttc gcgcttcctg aatggagaga
     2701 agatcgttat caaggtcagt cataattatt caacctcggt ggtcgagtag cgagtgttcg
     2761 tgcgagccta tcgagaccac catgttacaa gagcattttc tcaaacataa attttttgct
     2821 tcatagatgg tctggatacg cccctttaca gtgcattcgg ggctactcga ccatcagcat
     2881 tctagtaatt tatcgggtta atggctacct ctaaaacttt attctgaaac gcaaccgctg
     2941 cggctttgca ggcgggttga tcaccggtaa gcatcacgcc catgtaattg gtttcggacg
     3001 gtggcatggt cagagcgacg atactcacgt ctgccgcttt aagcgccgca tccagcgcaa
     3061 ccaagccttc gttgggtgta gcgaccagat acgcaatcgg ggtcccgacc gggatattgc
     3121 agacctgtga taggtaggtt cccgtcctcg aaacgacatg cggaaaaaac gcaatcgagt
     3181 catcctcatt tgcagagtac cagatggcct gtgttttgat gtaatcgacc gcggcactga
     3241 gcccggctct cacttcagcc gggtctggtc ccgcgatgat cgccatgatc tcacctgacc
     3301 attttccgga cgcgtgtttc gcacccgcat aaaaggacct ggcgtacaca acctctacat
     3361 ttgccatctt tgtggcttca tctatggcta cgtacgtcgc atcgtcattg tccacgctca
     3421 gcagaccgat cgagcgttgg tcgtcccgaa gtttgaggtg ttctgcaaag ttacgatcca
     3481 cctgtgggat gagctgaact gcgagagggg tgccgtaaag agggtcgagg atgggcatga
     3541 aggatcctta ctttgccagc ttcaatttta cgccagaagc ctgatgcttg cgcatctttt
     3601 gcgcgatttg cacgacgaac gctccagctt ccaacggatt ggttccgccg ttctcaaaaa
     3661 tgttgcagac aacatcgcga tcagcgtccg tgtctccata cttgggtttg tagcccatgt
     3721 aggcgctcat ggactcagcg cgccctaagc cggggcgttc accgatcagc aggatgacga
     3781 catctggctt gatcaattca cccacgtcgt tcatcacacc gacgcgcgca tatttgataa
     3841 agaagggcgt acccagtgta agtcctgccg tctgtacacc ttgcttgatc accgggaaca
     3901 tctgacgcag gtttgcttca actgcggcag cagataaacc gtcgcccacc acaagctgaa
     3961 catttacatt cttctggcat ttctcattga tggtgcgttt ggcatcgtca ttaagcagtc
     4021 gtccgagatc gggtcgaaga agatattcct gttttccgcc agtgatcctg gtttgcacgg
     4081 taaacaggtt gaattcatct aacagccttt ggtcgacgtc tcgatacagc gcgtcctgag
     4141 tgacggcgtg atcgccttgg aacagtaaca gtgaggccgt gctgtaacgc gggcctgcac
     4201 gaccaacgcc gatacgagct gtcgtggatg ccatcagcgc ccgtaaacct tgagcatctt
     4261 ttggattctt gacgcgcggt gtgttacgtg cttcactcgt agcagggtcg ggaaggtcga
     4321 tttgcaaatc accgccagtg actgtggcgc gcggtttgac cggaacaact gacgctgatg
     4381 ataacgtcgt tgaaacaggc gaaggagaat tgtctgaact cgaagcgccg ggcttcacag
     4441 ctccggattc ttttagctca cgtacaatgg cgtcgacgat cgcggcaagt tgagtgtcgt
     4501 ccataattat ctctttaaga aaatggttgg gtcgccggct ttttccgtaa gtacgccatc
     4561 cttcatcaac cctaaatcta ccatccattt ttcgaattcg ggggccggac gaagcttgaa
     4621 gagctggcgt aacgtgggtg cgtcatggaa ggacgtgcat tgatacgata gcattgagtc
     4681 gtcgcccatg ggcacgccca taaaataatt gcaacctgcg gcggtaagca tcacggcaag
     4741 attttcgatc gcgttctggt ctgcacgggc atgattggta tagcaggcat cgcaacccat
     4801 gggaatgccg gtgagtttgc ccatgaaatg atcttcaagc cccgcacgtt ggatctgacg
     4861 agcgtcataa aggtattctg gaccgatgaa gcccacgaca gtgttgacct gatacgggct
     4921 atatcgtttt gcaaaaccat agcaacgtgc ttccagcacg agctgatccc agccattgtg
     4981 ggcatcggca gagagttcgg aaccctgacc ggtttcaaag tacatgtagt tgggaccttt
     5041 ggggaaacaa tatttcttcg ccatctcgtc tgcttcatcg atcataccaa ccgagatgcc
     5101 gaacgagtcg tttccttttt gtgaacccgc aagtgattga aagaccaacc ccactggcga
     5161 accagactgc atacacttca tctgcgtggt gacatgtgaa agcaagcagt tttgggtcgg
     5221 gatgttccag gttttgatga catcataggt catgtcgagc aggcgagcca ctgatccata
     5281 cgaatcgtcc accgggttga tgccaattac cgaatcgccc gagccatagg acaagccttc
     5341 ataaatttcc gcgcggatac cttccggcga gtcggtgggg tgattgggtt gattccgcga
     5401 cgataatgta cccggcgcac caattgtatt gttacaatgc gcgctgacgg gaatcttctt
     5461 cgcagcgtac attaagtcga gattcgacat gagtttggtg acaccggcga tcatctcagc
     5521 cgtaagcccg ttcgaaacgc gacggatcat gtcagtggag gtggtgtcgg caagaatcca
     5581 ttccctgaat tcccccacgg tccagccttt gatttcgtta taaatcgatt ggtccacggc
     5641 gtccatgatg acgcgggtga tttcgtcttc ttcatatggc acggcggggt tgtcatgcaa
     5701 gacccacagg ggtacttctg ccagcaccaa ttttgcagcc acgcgttcgg ccgccgtctc
     5761 tgcgccgaca cccgcgagac gatcacctga tttctcctcg ttggctttgc ccatcaggag
     5821 gcgaatgtct ggaaattcat aggtcttgcc gtgcagtttc gtgcgcagaa gcataaaggc
     5881 tccttggacc tactagagta tcttacttat ttcttgagcc ggtaaaagct gtaagaacac
     5941 ccaaaccaat ataaattaac cctgcaaagt acctctggtt tctggcgaaa ttatcgttgg
     6001 agttcagttt atcggcaagc gaactggaaa ccagagcata cgtgctgtct gtgatcagtg
     6061 ccatcaagac gaacataatt ccgagcaaaa acatctgcaa cgtgacattt gtcagcgatg
     6121 tgttcacaaa ctgaggaaga aatgcgaaaa agaacaatgc ggttttcggg ttgaataaat
     6181 tgacgataaa gccttgcgaa taaatgcgcg agagtttttc ttgttgagct aatttggctt
     6241 tggtttcctc ccccttcgac aaaattttcc gaacgcccag gtaaatgaga tagccagcac
     6301 ccagatattt aaccaggttg aaggccagcg cagaggacag caatatcgcc gagagaccca
     6361 gcgcggcggc agaggcatgt agaaaattgg cagtctcgac gcccaggacg gaagcgatgc
     6421 cagccatttt tccctgattc atgctgcgtg ccacgatata taaaaccgcg ggaccagggg
     6481 tgattaggag tgcaaaagat gcgatgagga acaaatatag ctgcgatgat tcaaacattc
     6541 aatagcctca ggtggtcatt gttcccgccg atttggagcg aagaacgaac acttgcacct
     6601 cacgcacacc gccccggtgg ttcggtgccg gggagtgcgg gtgtccagac cacacactca
     6661 aagtttctta tttgcttgtt atttctggtc tcgactactt gaatttcgac cagaggatga
     6721 tgcaaaaaaa ttagaccttg atgacttcaa tctgatcgag tttcactggc gtgaggactc
     6781 gcaatgtatt ctgaatttca gcaacgctat ccgtaaactt cattatctca cggacttcgt
     6841 cttcgctggc ttgggaaaag actttggctt gataagtcac gttcttagca ggctcgccct
     6901 ctgcaccgaa ctcaccttcc acgttgactt cgatgtggtc aacctggatg ttcctttttg
     6961 cagcttcgcg gtaaacatca ttacagtagc aagtggctaa cgccagaaaa agtagttcgc
     7021 ctccgttcgt gctggaacca tacctggtgg atttcggagg gataacaatc gagtggacat
     7081 tgtcgatggt ttgcagcgcc acatgatgat tgtccttggt gttttctact tttgcgctaa
     7141 ttttcatatc ttaatttcat atggtggttg agtagtccca tgttctttgg ggcgtatcga
     7201 aaccacactt tgcaatttct tgtttacttg taattggtag tctcgataca ggcttcgccc
     7261 tactcgacca ccgatatata cctaatgata aaaaatcaac gtctttacac tcaacgggac
     7321 cacgcgtcca tccattagcg gagtaccaat atcaatgtaa tcgccttctg acaagccaac
     7381 ctgatcgatg acaagcagtg agcggtctgg cgcgagccct ttcaaggtct gacccagcgc
     7441 ctgagcataa tccctttcta taataacaac cagcggtcta tcggctggca tggtgctgga
     7501 aaattctcgc aagccattcg ccacttgtgt gagcaaagta tagtctaagg cgcggttgag
     7561 ttcaagcgca attgcgaagg ggtcggtgac gaggttcaca tcccagcggc ggactgcttc
     7621 agaaatggct gtggatattg ccaaaggctc aagcgtcgct ggtagcgccg gccggatcac
     7681 aggcatattt ttgatcggga ggatttcctg ctctgcccag atggtcgaac ctgagagggt
     7741 caccgtctgg gtacctgcgc ccaataccgt ggcgcgcaac gtttcgcttg gctgaacaat
     7801 ttcataacta ttcaaaattg aatgtttgcg catggattcg gctaacaacg ggccgacgtc
     7861 atcatgaatg gtggcatcgg caacagaatt gatctggatg gggttgtaaa agtaatgccc
     7921 aatgccacct gaaaacataa gcactgtacc ttttccagag acgcccaccg gcggcgtgag
     7981 atacaacttt tgggcaagcg gcgacgaagt accctcgact aattcgacgg tcatatcagc
     8041 catgcggtct gtaaatcgtc gtaactcatc cagcgaaggc gagtccccaa tagctagtgt
     8101 aaagccaata tcatgcaaga tatgttttgc agggtcagcg atgtgcctta cacggcccga
     8161 ggtatgttct atttctaaaa tccgcccgcc ataattcatt gccgcagcgc caatgggatt
     8221 gccgctttga aacgtaacgc tgtttgcgct tccaccgccg atgtcaatat tggtcacggt
     8281 cacataattt ttttgtgaat attcagccgc gcccgcaccc cgccctgaaa tcagactttc
     8341 cacattcgga cccgccacac tcacaacgaa atcgcccgcc agaccagaaa gggcgcgcag
     8401 gatctcatcg gcgttctttt tctttgcagt ctcacccgtg ataatgaccg cgcctgtttc
     8461 tacctggctc gggtcaattc cagcggaggc atactcgccg cgaacgatat cattcaggcg
     8521 atctgcatca atcgtttctg catcaatcag cggagtgaag acgatagggc tttgatagat
     8581 gaccttccga tccgtgatgt tgatgcgcgg gatttgtccc gcccgtgcca catcctgaag
     8641 atttattcgc gaaaaaataa cttgagtcgt ggtagtgccg acatccagtc cgacggacaa
     8701 aagggcgcgg gaatcgctca taagtgaaat aaagtatgca gcggatggca gctgccgtcc
     8761 gctgcatgga aaaatctatt cttttctata ggaaaccttg ccgcccaatt ccagtgaatc
     8821 aatcaccccc acgatgacgg cgtcaactgg cgcgtttgct gtttgattcg tgtagcgggc
     8881 agaggatcca tcggtgacca taacgagatc ccctgcacct gcgcttaccg tatccactgc
     8941 gatgtaagga tcgccagtta cattcccttc cgtatctgca tttcgcacga tgagaagttt
     9001 ccgcccccgg atcacttcat cttttatcgt cgaaatggtc gaaccgataa caagtgctat
     9061 tcgcatagaa gctcccaaaa ctgaattgga ctgcctcaat caattacatc taatttacca
     9121 tagaataaaa acacgaacaa tgtccagggt tagaagaaac aaccatatcc tacgcgcgac
     9181 ccggggaatg cctgaaacca aacttcatcc tgcatcccta tggaatgcct tggcggcgtt
     9241 cagcgcctta ttccatagag ccaatttctc aactctttct tcggctttca tgtttggtgt
     9301 ataaatcaac tctgctttcc aatggcttgc taattcatcg agtgaggtgc ccagtgcgga
     9361 tacgcccgcc agatttgcaa tgccgatggc tgtcgcctcc aatgcggcag atacatgcac
     9421 ctcaagttcc aacaggtccg cgatcatttg cataagatac ggattgcctg acgggccacc
     9481 gtccactttc aggtgcgggg gattttggcc tgcatcctgt gccatggcat ggaccacctc
     9541 atacactcga caagcaattc catccagcgt cgcccggacg atatcatccg aactcgtact
     9601 tcgattaagt ccaaacattg cgccccgcac gtccgtgcgc caatgcggcg cggcgagtcc
     9661 ttgtagggcc ggaacaaaga caacacctct gtcagtggat ttcaacgcag cgttgttact
     9721 ggcggcggca tccgggatta atttcaaatt atctctcaac cactgcaccg ctgagccggt
     9781 gacaaagatc ccgccatcaa aggcgtaggc agtatgccca ttgaaattcc aaccgacggt
     9841 agtaaccaat ccattttggg ataattttgg ttggtcaccg atattcatca acaggaaact
     9901 acctgttccg aaagaacatt tcatttcccc tgcgttgaag caggcctgcc caaacagggc
     9961 ggcttgttga tcaacaagca gggcctggag cggcaacggc tttccgttgc caaaatctac
    10021 atcaccaata aaactggctg atggttttac ttcgggcaat atgcttcgtg ggacatcaaa
    10081 taacgtcagc aactcctcat cccactcaag ccgattcatg tcgaacaaaa gtgtacgcga
    10141 cgccgttgac ggatcggtga catgccactt gccattggta agagaccata gcacccaggt
    10201 ttctgtcgtg ccaaatttga gtttcccagc atgtgcctgt ttgcgaattt ccggatcgtt
    10261 ttcaaatacc catttcagct tgggcgcgga gaaatagctg tcgagcagta gc
//
LOCUS       DASTJP010000446         8432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_683143, whole genome shotgun sequence.
ACCESSION   DASTJP010000446 DASTJP010000000
VERSION     DASTJP010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683143"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2204
                     /locus_tag="VFQ23_12895"
     CDS             <1..2204
                     /locus_tag="VFQ23_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET9907541.1"
                     /translation="ALTHYEKALSLAIAQQETRNIARIESSIAEIAQAQGHYRRALAL
                     LNSGLERVQTRLIFDATLIQHHIVECYLALNRNEEARKLAKTVVENCRTINAQYELAR
                     TLMFLATIEASLSNFSAAAAALKEAETIFDSRGATSWIATIRLWRGRIALKQGNPMLA
                     YQESLAAASAFEADKQQVNHALANLLNGQSLFAMGDLLASENAGNQTRHFAKRHNVPT
                     LRYEAYLLLGQISEARGSHMNAIHCYKAAAATIERVQRELTITLRPGFLEDKGEALRR
                     LISLYLQTGRTVKAFETLENAKAQNWLSYLNNRERLHWSREDATSRALIEKLEGLRAE
                     HQWFYQLSHHQPGNTEEFPKAVSQEKARNEMNDREQQMRVITEQLYLISEDHPYSKQA
                     VAPPFREIQRALENDTTLIEYYNDGESIWAFKLNKHEIQVHHLPLSVNVLSDLVERWQ
                     VNVSAALKMDPYAADTRKMTMLARRILQRLHALLIQPLELKRKSGRLLFVPYGALHFL
                     PFHLLHDGNEYLIEKHEILTLPAAGLVARKASKRDPGVLALAHSWDGKLPHTTNEAQG
                     VQKLFGGRIYAEEQASRDALQARPVQILHIATHGQHRLDQPDLSFLQLANGQLYADDV
                     MQEDLSYELVTLSACETGRARVMASDDLIGIGRSFLYAGAGALVSSLWQVGDKATLGL
                     MERMYAALYAGASKPSALRQAQMSILAENRDIHPAFWGAFQLVGNADPLSR"
     gene            2231..3913
                     /locus_tag="VFQ23_12900"
     CDS             2231..3913
                     /locus_tag="VFQ23_12900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HET9907542.1"
                     /translation="MIAPNIGNNEHERYVFVADEVVFQVLHPPVTDRESEAAMRARII
                     AAIVNFLRPGGGIEIGLQLAENDWKNRLAPPTANSRIVTFSSLTQLEGSERFSLIPIK
                     LIRPDPQSFTQADVINLLKDIYTDLRDSFIASNGDDDNDDEDGNDDKKDNREGKDDRE
                     EERDEEDRGNKDDRLRPIPLDDTGEISLKSISPNWLASDLHHGGATGGPGSLPASKPA
                     PSAVPPQFLLEGPIDSLANYRRPDAQIAILDTMQPANFPASVQAIFSANPSMNVVPYI
                     YPDLTPLSSYAQLPHHYQMPDHGTFIASIIRAIVPTAQVYLYEVLNSYGVGCFISIAQ
                     GVVDAVIQRGTNTPALILNCSFMLDDDLQDLDIEMEILKRRNIRNLLRTSMRDVFDWV
                     TRTSNLNVVVVASAGNDSLQGNRKDARYPAAFNDVVSVAALPKNYPKSSGKYRYASYS
                     NRSHSREPNKGSAYSIATFGGELISGSSSPREGVLGVYIGEVPTQNSDGTFSQTPPAN
                     VTSWAEWSGTSFSAPIISGLLALPHIPQVTATTSSDPYLTTDDGHVIRVRQG"
     gene            4058..5701
                     /locus_tag="VFQ23_12905"
     CDS             4058..5701
                     /locus_tag="VFQ23_12905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HET9907543.1"
                     /translation="METNVLGIVIVILVVIIVALLFWRLGRQEPDHPKPERPTPTKEY
                     YFSPDEVVFKIRHSEAALPQGIETTVVAAVRGFLGGDKSTERASVNFTRPTSERWKEM
                     LEMPQAATANVITLPFTNERGMPFSLVRVPVKAEFLEKDSDQTVREILKGAYADLQQN
                     NLPDLAGGYSIEDFSLNWLFSSLHHGGATGGPGGPPTEATPPTGGVQSFRVVSQITSL
                     ANAINMPTQTNAYVAILDTAPTSEAVVDTYHNKWPKHLGIERLFGTNFTGGRLTIHPA
                     NLATLYSMEHFSPMLHNYLMPDHGTFVAGIINSGAPDADLHLYQVLNRYGVGTFTSIA
                     QGIRDAIRDLAHLGRPLIINCSFQFCLPDGTYQAALITDLGRPLAGLTYSMRQLFEVE
                     ILKYSNIMVVASAGNNSDNSANPPNRVDARFPAAFNNVIGVGALPNGNPTTNNIYTPA
                     SYSNFADDPHGTGFMTLGGEPGTGNGIRGIYISDFPGSTNNNQTGWAWWAGTSFAAAI
                     ITGWLAGELSSNSGFLPPTSTPQLGQTLDGEAVILIQQP"
     gene            5836..6036
                     /locus_tag="VFQ23_12910"
     CDS             5836..6036
                     /locus_tag="VFQ23_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907544.1"
                     /translation="MNNRQEKDIWLYRIVVVVLGLILVASVVGALALVVSGQSTPQVI
                     VASAVIGGLAGLLASSSLNRNG"
     gene            6158..6304
                     /locus_tag="VFQ23_12915"
     CDS             6158..6304
                     /locus_tag="VFQ23_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907545.1"
                     /translation="MLRQILITLVMGTLLLGAAATGTSLPVLMLMGSLLLIALMETSA
                     RRMR"
     gene            6507..7028
                     /locus_tag="VFQ23_12920"
     CDS             6507..7028
                     /locus_tag="VFQ23_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907546.1"
                     /translation="MNKYDGKLEATFPEPYLYGDVSAKIYEKDGAKPTTIIRTDQEWG
                     VKIYWELKGSLAQYICGEWCIRVCLESIGHGPERNWEYRIPLDPCGNGQYYYDFKFKP
                     GDITADYCSTPYKPVVTVTYNSVCHVPGPVAGFVELPILQFYEVDRPNYGNNGHDSYP
                     DNREYEVEQEQAR"
     gene            7419..8366
                     /locus_tag="VFQ23_12925"
     CDS             7419..8366
                     /locus_tag="VFQ23_12925"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9907547.1"
                     /translation="MILVTGATGFIGRALVRHLSETGEQVRVLLRSSPNSPRLPRGVP
                     VEVAVVSLNDERGVRAALRGVDHIYHLASAGSQGRRGNLFNTDIEGTRVLAQAAKDAG
                     IKRLIYPSHIGADRASAFPIQKAKGIAEEHIRRSGVPYTIVRSSIVFGPEDRFTNNLA
                     RLLRTVPGFFPIPSDGRTLLQPLWVEDLVTCLTWALHNSEMVNQTYEVGGSEYFTLRQ
                     ILETLMNVTHARRMFLPLPTPYMRALLVLLDNFIPNFNISTYWLDYVAVNRTCPVDNL
                     PRTFGLMPARFAYRLEYLARKPWYSDFAKRIQDAFRLSR"
BASE COUNT         2201 a         1946 c         2098 g         2187 t
ORIGIN      
        1 aggccctgac acattacgaa aaggccctgt ctttggctat tgcccaacag gaaactcgta
       61 acatcgcccg aattgagtcg agcatcgctg agattgcaca agcacaaggt cattatcgtc
      121 gtgctcttgc tttgttgaat agtggattag aaagagttca gaccaggttg atatttgatg
      181 ctacgctaat tcaacatcac atagtggagt gttatcttgc gctgaaccgc aatgaagagg
      241 cgcgtaagtt ggcaaagaca gtagttgaga attgccgaac tattaatgca caatatgagc
      301 ttgcccgaac attgatgttc ctggcaacca tcgaagcttc cttgagcaac ttttccgctg
      361 ctgctgcggc gttaaaagaa gctgaaacca tcttcgattc tcgtggtgca accagttgga
      421 tcgcaacgat tcgtctgtgg cgtggtcgca tagcacttaa gcaaggtaac ccgatgttag
      481 cttatcaaga atcattagct gccgcatccg cttttgaagc ggataaacaa caggtcaatc
      541 atgcactggc aaacttattg aacggccagt ccctcttcgc gatgggggat cttctcgcct
      601 ctgaaaacgc cgggaaccaa actcgtcact ttgctaaacg tcataatgtt ccgaccttgc
      661 gttatgaagc ttatctgttg ctcggacaaa tttctgaggc gcgtggcagc catatgaatg
      721 ctatacattg ctataaggca gccgcagcaa ccattgaacg tgtgcagcgc gaattgacga
      781 tcaccctccg ccccggtttt cttgaagaca aaggggaagc tttgcgtcgc ctgatttctt
      841 tgtacttgca aacaggtcga acggtgaaag cctttgaaac attagagaac gccaaggcac
      901 aaaattggtt gtcgtattta aataatcgtg agcggctgca ttggtctcga gaggatgcta
      961 cgagccgggc gttgatcgaa aagttggaag gtttacgggc cgaacatcaa tggttctatc
     1021 agttgtcaca tcatcagccg ggcaacactg aagaattccc caaggcagtt agtcaagaaa
     1081 aggcgcgcaa tgaaatgaat gatcgtgaac agcaaatgag agtgatcacg gaacaattgt
     1141 atctgatcag tgaagatcat ccttactcta aacaagcagt tgcaccgcct ttcagagaaa
     1201 ttcaacgagc gttggaaaat gacacgactt tgattgaata ttacaacgat ggagagtcaa
     1261 tctgggcatt taaattgaac aagcatgaga ttcaagtcca ccatttacca ttgtcagtta
     1321 atgtcttaag tgacttggtt gaacgatggc aagtaaatgt ttcagccgcg ctaaaaatgg
     1381 atccatacgc agctgatacc cgaaagatga ccatgcttgc tcgacggatt ctgcagagat
     1441 tacatgcttt gttgattcag cctcttgaat tgaagcgtaa aagcggtcgt ttgttgttcg
     1501 tgccctatgg tgccttgcat tttttgccat ttcacttact acacgatggg aatgagtatc
     1561 taatcgagaa acatgaaatc ctaaccctgc ctgctgcagg attggtggct cgcaaggcct
     1621 caaagcgtga tccgggagtg ttggcgcttg cacactcatg ggatggaaaa ttaccacata
     1681 cgacaaatga agcgcagggg gtgcagaaat tgtttggtgg aagaatctat gcggaggagc
     1741 aggcaagccg agacgcctta caggcaaggc ctgtgcagat tttgcacatt gccacacacg
     1801 ggcaacaccg cctagaccag ccagatctgt cttttttgca gctcgccaat ggtcaactat
     1861 atgccgatga tgttatgcaa gaggacttga gctacgaact ggtgactctc agtgcctgtg
     1921 aaacaggtcg tgcgcgtgtg atggcctcag atgatttaat cggaatcggt cgtagctttt
     1981 tatatgcagg tgccggtgca ttagtttcta gtctctggca ggtaggcgac aaagctacac
     2041 tcgggttaat ggaacgtatg tatgccgcgt tgtatgctgg tgcatccaag ccttctgcac
     2101 tgcgccaggc gcaaatgtct attcttgctg aaaacagaga tatccacccc gcgttctggg
     2161 gtgcatttca attagtgggg aatgcagacc cgctatctag ataatcgact caaacggaag
     2221 gaaaaatatc atgatagctc ccaatatagg aaataatgag cacgaaagat atgtttttgt
     2281 agcagatgag gttgtgtttc aggtacttca tccaccggta actgaccgtg aaagtgaagc
     2341 ggcgatgaga gcgcgaatta ttgctgcaat agttaacttt ttacggcctg gcggaggaat
     2401 tgaaattggc ttgcaactgg cagagaatga ttggaaaaac agactcgcac cacccaccgc
     2461 gaattcgaga atagtaactt tttcctctct aactcaactt gaaggttccg agcgattttc
     2521 tttgattcct attaagttaa taagacctga tccgcagagt tttacgcagg ctgatgtgat
     2581 caatttgctg aaagatatat atactgacct gagagacagc tttattgcaa gtaatggaga
     2641 tgatgacaat gatgatgaag atggcaatga tgataagaag gataacaggg aaggaaagga
     2701 tgatcgtgaa gaagaacgag atgaagaaga tagaggaaat aaagacgacc gattacgacc
     2761 gattccatta gatgatactg gcgaaatcag tttgaaatcc atctcgccta attggcttgc
     2821 tagtgatctt catcacggag gtgccactgg tggaccgggt agtttgcctg ccagcaaacc
     2881 tgcaccatca gcagttccac ctcagttcct tcttgaggga ccgatcgata gcttggcaaa
     2941 ttatagacga ccagatgcac aaattgccat cctcgacacg atgcagcctg ctaattttcc
     3001 agccagtgta caggctatat ttagtgctaa tccaagtatg aacgttgttc cttatattta
     3061 cccggattta actcctttga gctcatatgc tcaactgcct catcattacc agatgcccga
     3121 tcacggcact ttcattgcga gcattattcg ggcgattgtc ccaactgcgc aggtttatct
     3181 ctacgaagtc cttaattcgt atggcgttgg ttgtttcatt agcattgcac aaggggtagt
     3241 ggatgctgtc attcaacgcg gtaccaacac tccggcactg attctcaatt gtagttttat
     3301 gcttgatgat gatttgcaag atctagatat cgagatggaa atacttaaga ggcggaatat
     3361 ccgtaaccta cttcgaacct ctatgagaga tgtatttgac tgggtgacga gaacaagtaa
     3421 cctaaacgta gttgttgtgg cttcagctgg taacgattct ttgcaaggaa atcgaaaaga
     3481 tgcgcgatat cctgctgcgt ttaatgatgt tgtgagtgta gctgcacttc caaagaacta
     3541 tcctaagagc agtggaaaat atagatatgc ctcttactcc aaccgatctc attcacgcga
     3601 accgaataaa ggtagcgcat acagcattgc aacttttggt ggcgagctga taagtggatc
     3661 gtcatcacct cgagagggtg ttttgggggt gtatattgga gaagtcccaa cgcagaacag
     3721 tgatgggact ttttcgcaaa cgccacctgc taatgtgaca agctgggcag agtggtcagg
     3781 cacatccttt tcggctccaa taatctcggg actattggcc ttacctcata ttccccaggt
     3841 cactgctacc acgagctctg atccgtattt gactactgat gacggacatg tcatccgtgt
     3901 acgacaaggt tgatatgctt ttccaccggc cacctacaag aattcgcttg tgggctggct
     3961 tttatctcgg cgcatgaaaa aaatggaaac ttgcgttcat agagatgact aaaccatgta
     4021 atgcgacatc ttagatcatt caggaaggag aaggatcatg gaaaccaacg tgcttggtat
     4081 cgtcatcgta atactcgtag taataatcgt tgcgctcttg ttttggcgcc ttgggcggca
     4141 agagcctgac catccaaagc ctgaacggcc aacgcctacg aaagagtatt atttttcgcc
     4201 tgacgaggtc gttttcaaga tcaggcatag cgaagccgcg ttaccgcagg gtatagaaac
     4261 taccgtggtg gccgcagttc gaggtttctt gggtggtgac aaatccaccg agcgggcctc
     4321 cgtgaatttc actcgaccga ccagtgaaag atggaaagaa atgctcgaaa tgccgcaagc
     4381 agctacagct aatgttatta ctcttccgtt cactaatgaa agaggcatgc ctttttcgct
     4441 tgtccgtgtt ccggtgaagg ctgaattcct tgagaaggat tcggatcaga ccgtacgcga
     4501 gattttgaag ggcgcctatg ctgatctaca acaaaataat ttaccggacc tagctggtgg
     4561 ctatagtatt gaggattttt ctctcaactg gcttttcagt agtcttcatc acggcggtgc
     4621 tacaggcgga ccaggtggtc caccaactga ggccaccccc ccaacaggtg gcgtacagag
     4681 cttccgagtg gtcagtcaga tcaccagctt agctaatgct atcaatatgc ccacgcaaac
     4741 taatgcgtac gtagctatcc tcgataccgc gccaactagt gaagcagtag ttgatacgta
     4801 ccataataaa tggccgaaac atttggggat tgagagattg tttggcacaa acttcacagg
     4861 tggaagattg acgattcatc ctgccaatct agccaccctc tatagtatgg aacattttag
     4921 tcccatgtta cataattacc tgatgccaga tcatggcaca tttgttgctg gcattattaa
     4981 cagtggtgcg ccagatgcag acttacatct atatcaggta ttgaatcggt atggcgtggg
     5041 gacctttact agcattgctc aggggattag agatgcaatc cgagatttag cccatttagg
     5101 tcgtccgctt attattaact gcagtttcca gttttgtctt cccgatggaa cttatcaagc
     5161 tgccctgatt accgacctgg gtcgaccatt ggcgggattg acatattcaa tgcgacaact
     5221 attcgaagtg gaaattctta agtactcaaa cattatggtt gttgcatctg caggtaataa
     5281 ttcggataac tcggctaatc caccgaatag agtcgacgca cgttttcccg ctgcttttaa
     5341 taatgttata ggtgtaggcg cgctaccaaa tggtaatcct accactaata acatttacac
     5401 tccggcatcc tattccaact ttgcagatga cccgcatggg accggattca tgacgctagg
     5461 cggcgagcct ggcacgggca atggaattcg agggatatac atcagtgatt tcccaggatc
     5521 aacgaataac aatcaaacag gctgggcctg gtgggcgggc acatccttcg ctgcagcgat
     5581 tatcactggc tggctggctg gcgaattgtc gagtaatagt ggatttcttc cccctacttc
     5641 tactccccaa cttgggcaaa cactggatgg tgaagctgta attctcatac agcaaccata
     5701 acctgaaaga tccaaccaaa ggtaatttgc cagcccggaa gattcgcttc tgggctggtt
     5761 cgtttaagtt atgcaatgcc tgaaaaccat ccaaactttc gttcatactg atagacagaa
     5821 tcaaaaggaa ctgcaatgaa taaccgacaa gaaaaagata tctggctgta ccgcatagtt
     5881 gtggtagtgc tgggcttaat cttagtagcc agcgtcgtgg gtgcgctcgc attggtagta
     5941 tctggccaat ccacaccgca agttattgtt gcatcggctg tgatcggtgg cttggcgggg
     6001 ctcttggctt cctcatccct aaacagaaat ggataatagc gatgaaaaga gcaaccgcct
     6061 gggagcccct gatcagccaa caggggcttc atctttttaa gtctcgatac ttgaaaacga
     6121 gacgttcctt cgttgaatgg agagaggaga aattgacatg ttacgacaaa tcttgatcac
     6181 gcttgtaatg ggaacgctgc tgttgggcgc agcggccacg ggaacttcgt tgcccgttct
     6241 gatgctgatg ggcagcctgc tcttaattgc actgatggaa acctctgcgc gacgaatgcg
     6301 ataacaaagc agcagacacc gacatataga ggatttcctt tatttttgaa aaagaatatt
     6361 ggaaatcctc tgagccctgc gtttatgaaa gtagacaacg tgaatgtgct gaggagtgaa
     6421 cgggcccttc atgaggcggc acgggaacgt gcgagaagac tcgccagagt catcaacatt
     6481 tctgatatca aaaatggagg attactatga acaagtacga tggcaaactt gaagcgacat
     6541 ttcctgaacc ttatttgtac ggagacgtgt ccgcgaagat ctacgagaaa gacggcgcga
     6601 agccgacgac catcattcgc accgatcagg agtggggagt caagatctac tgggagctga
     6661 aaggctcact tgcccagtac atctgcggtg agtggtgtat tcgcgtgtgc ctggaatcga
     6721 tcggtcatgg accggaacga aactgggaat atcgcatccc tctcgatcca tgcggtaatg
     6781 gtcagtacta ctatgacttc aagttcaaac ccggggatat caccgctgat tactgctcca
     6841 ccccctacaa gcccgtcgtg acagttacct acaattcggt gtgtcacgtt ccaggtccgg
     6901 ttgctggctt tgtggaactg cctatcttgc agttctatga ggtagatcga cccaactatg
     6961 ggaataacgg acatgattcg tatcctgata atcgtgaata cgaggtggaa caggaacagg
     7021 cgcggtaacc cagtctgccg cacgaccctg cctgcgtagg caggcagggt cattttatta
     7081 tttgccgtga tgctttcaaa ggcttcacgg ctttttttaa gcacataatg cacgaaactg
     7141 gttatatcta tggcctttca ttttcaggag agtgtctaat ttcgtgggct tagccacaca
     7201 cctgccctgg ggggcggtgc caggggagcc cacagagtct tttgagtttt ttccacctgc
     7261 cccgaagcag gtgcggggtc aaggaactca gtgatccacc tgcactggca tccaggtgcg
     7321 agtgtctgtg gcaaaacaac tattagccca tactattgga cactcccctt ttcagcggct
     7381 ccaaagccga tacccgctcc tctgctataa tctttgccat gattttggtc actggcgcga
     7441 cgggttttat tggtcgtgct ctggttcggc acttatctga aacgggagaa caggtgcggg
     7501 tgctcctgcg ctcctcaccc aattctccgc gtctgccgcg tggggttccc gtggaggtgg
     7561 cagttgtcag tttgaacgat gagcgcgggg tgagggctgc actacggggc gtggatcaca
     7621 tctatcacct ggcgagcgcc gggtcacagg gacggcgagg aaaccttttc aacacagata
     7681 ttgaaggaac gcgtgtatta gcgcaggccg caaaagatgc aggcatcaag cgcttgatct
     7741 acccaagtca tattggggca gaccgcgctt cagccttccc aattcagaaa gcaaaaggga
     7801 ttgcggaaga acatatccgc cgcagtggtg tgccctatac catcgttcgt tcgtcgattg
     7861 tttttggccc cgaggatcgc tttacaaata atctggcgcg tctgttgcgc accgtgcctg
     7921 ggttctttcc gatccccagt gacggaagaa ccttacttca acccttatgg gtggaggatc
     7981 tggtcacttg cctcacctgg gcattgcata attccgaaat ggtcaatcaa acctatgagg
     8041 tcggtggtag tgaatacttt accttacgtc aaattcttga aacgcttatg aatgtgactc
     8101 atgcgcgccg gatgttcctg ccgttgccga caccctatat gcgcgctttg cttgtgttgc
     8161 tcgacaattt cattccaaat ttcaatatct ctacctactg gctggattac gttgctgtca
     8221 accgcacgtg cccggtggat aacctgcccc gcaccttcgg tttgatgccg gcgcggtttg
     8281 cctatcgcct ggaatacctc gcccgtaaac cctggtattc tgattttgcc aaaaggattc
     8341 aagatgcgtt tcgactctcc cgttaagagg gtagagacct taatgactta tttaagtcga
     8401 ccggatgcaa atatacaatg aaaggttgct tg
//
LOCUS       DASTJP010000447         6173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_264408, whole genome shotgun sequence.
ACCESSION   DASTJP010000447 DASTJP010000000
VERSION     DASTJP010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264408"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1812)
                     /locus_tag="VFQ23_12930"
     CDS             complement(160..1812)
                     /locus_tag="VFQ23_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9907548.1"
                     /translation="MPDYDLIVIGSGAGGLTAAVALAQAGRKVLVLEQHDRPGGWTHS
                     FTLNGYRFSPGVHYIGDLHEGGRLRNIYEGLGVSQDLTFCELNPDGYDHIFIGEKKID
                     FPKGKENLVERLKSHFPQEAKGIDSYFADVINMMESISRIGRLSNPFQALLSVPTVIK
                     WARASGVDLINAHINDPILRGVLAGQSGDHGLPPSQVSAFMHAAITHHYLDGGYYPLG
                     GAFTLPRAFVRALKRAGGEIRLKSRVKRIMLEGRKVIGVELESGEEIRAGVVISNADP
                     EVTFGQLIGKDKLSPRLKRKVDSARYSTSCLSLFFATDMDLRHDAGLDSGNMWFYDHA
                     DVDKIYSNGLTDAVLSDETPSGMFLTATTLKDPSKIHSGHHTCEAFAFVGYEAFEKWA
                     HTKYGSRPTDYDGMKEDLAWRMVRGLEKRIPGLSKHIVYYSLGTPLSNEHYLNATRGN
                     LYGIEKSSSQVGPWSFSPRTEFDGLYLCGASTLSHGVAGVTSSGIDAAKAVLHCRTRD
                     LLKQGGANLTFLPSEDVSVWPEYLRRKMERGEVAREEEEMEV"
     gene            complement(1843..2343)
                     /locus_tag="VFQ23_12935"
     CDS             complement(1843..2343)
                     /locus_tag="VFQ23_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907549.1"
                     /translation="MKNIAIITLATIMIFLSSCGLLKPIVLTDSEILNEFAGDYAFST
                     GYWSYSLSIRSDNTFRAEVFTDLGDHLVYNGGISIQNSQITLLLDDEASNFFDEVYLP
                     TRWERRKYLIDLDKIVYFCESIQSSELRTEMLNSPFGSFFLKRGVLEKYAGGVPISPS
                     GEAICH"
     gene            complement(2372..3238)
                     /locus_tag="VFQ23_12940"
     CDS             complement(2372..3238)
                     /locus_tag="VFQ23_12940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9907550.1"
                     /translation="MKPGVTVRKVSKPVWIVLGVLLLSLVAFLDYITGVEFSFSLFYL
                     LPISLISWAINARIGLVFAFLGLCVWIVVDIWSGNRISNLFAYIWNSTARLGFFLLPV
                     FMIRLNRALQREQELARTDFLTGVLNARFFHELAQMEIDRSIRYKRPFTIAFIDVDNF
                     KTINDTFGHTEGDAVLQAIAMNIKAHLRKTDIVTRVGGDEFVVLLPETSAQTAPVVIS
                     NMQRALLNEMNENGWFVTFSIGVLTLGDPRLSVDEMLGRADQLMYVVKNSGKNNIQYA
                     IHPAEESYALKS"
     gene            complement(3241..4233)
                     /locus_tag="VFQ23_12945"
     CDS             complement(3241..4233)
                     /locus_tag="VFQ23_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9907551.1"
                     /translation="MLKRTLGKSGLEVSALGMGCWAIGGPWTWAQPGREPYPAGWGST
                     DDAESIRAIHAALDSEVNFFDTAANYGAGHSEVVLGKALKGKRDKVVIATKFGHIVNE
                     QAKTVYGDPAQIIKNVRTDVENSLRRLQTDVIDIYQLHDGGYDSKLALELQSVLEELV
                     SAGKIRWYGWSTDIVDSARSFASGAHCTSIQFRLNAIYDNAEMRKVCADFNLAGINKD
                     PLNKGVLTGKFSSTSTFPTNDIRSRENFSNPEVMKRLKIVDEIRDILTSNGRTMAQGA
                     LAYIWGLDERMVPIPGFKSVKQVEDNAGAMEFGPLTEAQVKEIRTIVGKYELKS"
     gene            complement(4362..4444)
                     /locus_tag="VFQ23_12950"
     tRNA            complement(4362..4444)
                     /locus_tag="VFQ23_12950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4408..4410),aa:Leu,seq:caa)
     gene            4549..5607
                     /gene="ychF"
                     /locus_tag="VFQ23_12955"
     CDS             4549..5607
                     /gene="ychF"
                     /locus_tag="VFQ23_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET9907552.1"
                     /translation="MKLGIIGLPQSGKTTIFNALTRGHAPTTASAGRFEVHTAVVDVP
                     DPRVTTLSDMFKPKKTIYAKVTYADIAGLETGSAKSGISGQLLNQLTQMDGFILVVRS
                     FIDDSVHHPLGSVDPARDADVMLSELLLNDLIAVERKLERLTEERKKGGTDKADNERQ
                     TALFQKLNAALSENTVLRKVELSAEENKILSSFGLLTRKPVLIVFNMGEGQTAPEREF
                     ETPSVSLQGKLEMEIAQLSAEDAEVFMQEYGIEELSLNKMISLSYDLLQVQSFFTVGE
                     DEVRAWETKRGATAQESAGEIHTDLQRGFVRAEVVAYDDLISLGGMNEAKSKGKLRLE
                     GKEYLVKDGDIVHIRSSL"
     gene            5731..>6173
                     /locus_tag="VFQ23_12960"
     CDS             5731..>6173
                     /locus_tag="VFQ23_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET9907553.1"
                     /translation="MATVKSYTIKKWNLDELFTGFDSPELEGAFDNVEEQVTSFEGVR
                     SKLNPDMDPYTFLEVVRASESMSRLVNRIYAFAGLSFAEDTQNQSAQSLLGRVQQFAA
                     EMQNRTLFFGLWWKDLDADNAERLMSASGDYRYYLEEMRHFKPHT"
BASE COUNT         1615 a         1605 c         1418 g         1535 t
ORIGIN      
        1 atttaaagaa acaccctgct tcttggcaag ccgttctgcg tcccttttga gttcattggg
       61 gagattaaga gcgtatcgag tcatagcaac ctctgtttat gatgtcgtat atgacatcat
      121 aaatgatgtc tcatttttgt caacagttaa agaagctccc tacacctcca tctcctcttc
      181 ttccctcgcc acctcgcctc tttccatctt cctccgcaaa tactcgggcc atacgctcac
      241 gtcttctgac ggcaaaaaag ttaggtttgc gccgccttgc ttcaacagat cacgcgtgcg
      301 acagtggagc actgccttcg ccgcgtcgat gcctgaagag gtgacgcctg ctacgccgtg
      361 gcttaatgtg ctcgcgccac atagatacaa accatcgaac tcagtgcgcg gcgagaatga
      421 ccagggtcct acctgcgatg agcttttctc gatgccgtat aaattgccgc gcgtggcgtt
      481 gagataatgt tcattcgaga gcggcgtgcc gaggctgtaa tacacaatgt gtttgctcaa
      541 gccggggatg cgcttctcca gcccgcgcac catacgccag gccaggtctt ccttcatacc
      601 atcgtagtcg gtagggcgcg aaccatattt cgtgtgcgcc cacttctcga acgcctcata
      661 gcccacgaat gcaaacgcct cgcaggtgtg atgtccgcta tggatcttcg atgggtcttt
      721 caacgtcgta gcggtcagaa acatccccga tggagtttca tcactcaaca ccgcgtcggt
      781 gagtccattt gaatatattt tgtccacgtc cgcatgatcg taaaaccaca tgttgcctga
      841 gtcgagccct gcgtcatgcc gaaggtccat gtctgtggcg aagaacaggc taaggcagga
      901 tgtcgaatat ctggcggaat ctactttccg cttgagacgc ggcgagagtt tgtcctttcc
      961 gatcaattga ccgaacgtca cttcagggtc cgcattggag atgacgacgc ccgcgcgaat
     1021 ctcttcgcct gactccaact ccacgccgat cactttcctc ccctcaagca tgatccgctt
     1081 cacgcgagac ttcaatctga tctcaccacc cgcgcgtttc aacgcccgga cgaaggcgcg
     1141 cggcaacgtg aacgccccac ctaatggata atatccgccg tcgagataat gatgcgtgat
     1201 agctgcatgc atgaacgccg agacctgcga aggcggcagt ccgtggtcac ccgattgacc
     1261 cgccagcacg ccgcgtaaaa ttgggtcgtt gatgtgcgca ttgataagat ccacgcccga
     1321 agcgcgcgcc cacttgataa cggtcggcac gctcagtagg gcttgaaatg gattgctcaa
     1381 cctgccaatg cgagaaatcg actccatcat atttattaca tcggcgaagt acgaatcaat
     1441 cccttttgcc tcctgcggaa aatgactctt caaccgttca actaaatttt ccttgccttt
     1501 ggggaaatcg attttcttct ccccgataaa aatatgatca taaccatcgg ggttgagttc
     1561 acaaaatgtt aggtcttgcg atacacccag tccctcataa atattccgca aacgaccacc
     1621 ctcatgcaag tcaccgatgt aatggacgcc cgggctgaag cgatagccat tcaacgtgaa
     1681 tgaatgtgtc cacccaccgg ggcgatcatg ttgttcaagc accagcactt tcctgcctgc
     1741 ctgcgctagc gccaccgctg cagttaaccc gccagcgccc gaaccaatga caatcaaatc
     1801 atagtctggc atgaaactct ccaaaattct tgcagaaaga gtttaatggc aaattgcttc
     1861 acctgatggt gaaataggaa ctcctccagc atatttttcc aaaactcctc tttttaagaa
     1921 gaaagatcca aagggactat tcagcatttc tgtgcgtaac tctgatgatt gaatactctc
     1981 gcaaaaatac actatcttat ccaaatcaat aaggtacttt ctgcgctccc atcttgtggg
     2041 tagataaact tcatcgaaaa aattggatgc ttcatcatct agtaataatg taatctgact
     2101 gttttgaatg gaaattcctc cattgtaaac aaggtgatcg cccaggtctg tgaatacttc
     2161 agcacgaaac gtgttatctg atcggataga cagagaataa gaccagtatc cagtactaaa
     2221 agcataatca cccgcaaatt cgttcaagat ctcagagtct gtgagaacaa ttggttttaa
     2281 taacccacaa ctacttagga atatcataat agttgcaaga gttatgattg ctatgttttt
     2341 catttaattt tccctgttat ttatcgtacc ttcaagattt tagtgcatac gactcttctg
     2401 cagggtgaat tgcatactga atgttatttt taccgctatt tttgactaca tacatcagtt
     2461 gatcagcgcg cccgagcatc tcatccacgg agaggcgcgg gtcgcccagc gtgagaactc
     2521 ctatgctgaa tgtgacgaac cagccatttt cattcatctc attcaataag gctcgctgca
     2581 tattggaaat tacaaccggg gcagtctgcg cacttgtttc aggcaataaa acaacaaact
     2641 cgtccccgcc cacccgtgtg acgatatccg ttttacgcaa atgcgctttt atgttcatgg
     2701 cgatggcctg caggactgca tccccttcag tgtgtccaaa tgtatcattg atggtcttga
     2761 aattgtcaac gtcgataaac gcaatcgtga agggtcgttt atatcgaatg gaacgatcaa
     2821 tttccatttg agccagttca tgaaaaaagc gggcgtttaa aacacctgta agaaaatctg
     2881 tgcgcgcaag ctcctgctcg cgttgcaggg ctctgttcaa ccgtatcatg aaaacaggca
     2941 gcagaaagaa tccgaggcgg gctgtagagt tccatatata ggcaaacaaa ttggatattc
     3001 tgttacctga ccagatatct actacaatcc agacgcacaa accaaggaac gcaaatacca
     3061 gtccaatcct tgcattgatc gcccaggata tcaaactgat agggagcaag taaaacaacg
     3121 agaatgaaaa ttccaccccg gtaatgtaat cgagaaaagc caccagactt aagagcaaaa
     3181 ctcccaacac gatccatact ggcttgctga cttttctcac agtcacgcct ggtttcatga
     3241 tcaagatttc aactcatatt ttccgacaat cgttcgaatc tctttcacct gcgcctccgt
     3301 caagggacca aattccatcg cacctgcatt atcctccact tgcttcacag acttgaaccc
     3361 tggaatggga accatgcgct catccaaacc ccaaatgtaa gccagagcac cctgagccat
     3421 tgttcgccca ttggaggtga gaatatcgcg gatttcatct acgattttca atcgtttcat
     3481 cacttcggga ttcgaaaaat tctcacgtga tcggatgtca ttggttggga atgtggatgt
     3541 tgaactaaac tttccggtca agacgccttt attaagaggg tctttattga tgcctgccag
     3601 gttaaagtct gcacatactt tccgcatttc agcgttgtca taaatggcgt tcaaacggaa
     3661 ttgaatggag gtgcaatgcg ccccgcttgc gaaagaacga gcactgtcaa caatatctgt
     3721 gctccagccg taccagcgga tcttgcccgc cgaaaccagt tcctctaaaa ccgactgcaa
     3781 ttccaacgcc agtttggagt catagcctcc atcgtgcaat tgatagatgt cgatcacatc
     3841 cgtttgcaaa cgacgcaggc tattttctac atcagtccgc acgtttttga tgatttgagc
     3901 agggtcacca tagacagttt tcgcctgctc attgacgata tgtccaaact tagtggcaat
     3961 caccacttta tcgcgcttgc ctttaagcgc ctttcccaaa acaacttcgc tgtgacctgc
     4021 cccataattt gcggcggtat caaagaagtt gacttcagaa tctaacgcgg catgaatcgc
     4081 gcgaatcgac tctgcatcgt ctgtacttcc ccagcccgcc gggtatggtt ctcttccggg
     4141 ctgggcccat gtccatggac cgccaatcgc ccagcatccc atgcccagtg cgctcacttc
     4201 caatccactt ttgcccaggg ttcgttttag catgtttaga tatcctttta taggttgttc
     4261 gtacatttat acatatttac aaaaaatcct ccgtttcagg gaggatgttt tgcaccaggt
     4321 gctcatgagg gtataatgcg agcgaaacca aactcagcga gtgccgaagg agggaatcga
     4381 accctcattc ccgtagggaa cacgattttg agtcgtgcgc gtctgcctgt tccgccactt
     4441 cggctcttta atttgcgttc gcaagtatac cactcccaac atgaaggtca agacaaaatc
     4501 ttgtacggcg gaatgcgcga agcgtcccgc ccaattgcaa aggatcacat gaaactcgga
     4561 attatcggat tgcctcaatc aggaaagacc acgatcttca acgcattgac gcgcggtcac
     4621 gcgcccacga ccgcctccgc aggacgtttc gaagtccata ccgcggtggt ggatgtcccc
     4681 gatccgcgcg tcactaccct ctcggacatg ttcaagccga agaagacgat ctacgccaag
     4741 gtgacgtatg cagatatcgc agggctggaa acgggttccg ccaaaagcgg catctcaggc
     4801 caactgctga atcaactgac tcaaatggac ggttttatcc tcgtggtacg tagttttatc
     4861 gacgacagtg tccatcaccc actggggagt gtagaccctg cgcgggatgc ggacgtgatg
     4921 ctcagtgaat tactgttgaa cgatctcatt gccgttgagc gcaagctgga acgcctgact
     4981 gaagaacgga aaaaaggggg cactgataag gcggacaatg aacgccagac cgccctcttt
     5041 cagaaattaa atgccgcgct ttctgagaac actgtgttga gaaaggtaga actttccgca
     5101 gaggaaaata aaatcctttc cagttttggt ttactcacgc gcaagccagt cctgatcgtc
     5161 ttcaacatgg gggaagggca aaccgcaccg gaaagagaat tcgaaacccc atccgtctcg
     5221 ctgcagggca aactcgaaat ggagatcgcc cagctctctg ccgaagatgc ggaagtgttt
     5281 atgcaggaat atggcattga agaactcagc ctgaataaaa tgatcagtct gtcgtatgat
     5341 ttgctccagg tacagtcatt ctttacagtg ggcgaagatg aggtccgagc gtgggagacc
     5401 aagcgcggcg caaccgcgca ggaatccgcc ggtgaaatcc acaccgacct gcagcgcgga
     5461 ttcgttcggg cggaagtggt tgcgtatgac gacctcatca gcctcggggg gatgaacgaa
     5521 gcaaagtcca aaggcaagct acgactcgaa ggcaaggaat atctcgtcaa agacggtgat
     5581 atcgtccata ttcgatccag cttataaaat acacctcaaa atacatattt taaatctacg
     5641 aaagagtttg actttatggt aaaaatcata tatgtcattg cgagggagcg atagtgaccg
     5701 aagcaatgac atgagtcaaa ggagaaatat atggctacag tgaagtccta tacaatcaaa
     5761 aaatggaatc tggatgaact ctttacgggc ttcgatagcc cggaactgga aggcgcgttc
     5821 gataatgtgg aggagcaagt gacctctttc gagggcgtgc gcagcaaact caacccggat
     5881 atggatccgt acacctttct cgaggtggtg cgcgcttccg agtcgatgtc gcgcctggtt
     5941 aatcggattt atgcctttgc cgggctttcc ttcgcagaag atacacagaa tcaaagtgcg
     6001 caaagtttac tgggacgtgt gcagcagttt gcagccgaaa tgcagaatcg tacgttgttc
     6061 ttcggcctgt ggtggaagga tttggatgct gacaatgccg aacgactgat gtctgcttcg
     6121 ggtgactatc gctactatct cgaagagatg cgtcacttca aaccccatac att
//
LOCUS       DASTJP010000448         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1124636, whole genome shotgun sequence.
ACCESSION   DASTJP010000448 DASTJP010000000
VERSION     DASTJP010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124636"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1311)
                     /locus_tag="VFQ23_12965"
     CDS             complement(55..1311)
                     /locus_tag="VFQ23_12965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9907554.1"
                     /translation="MFLKLKLDATRVYLFMEMALAVFTCMVFVTNSLYQVTIAGLTPL
                     QLVLIGTTLEVSAFVFEVPTGVVADAYSRRLSIIIGYILIGLGFLIEGFFPAFWPIVL
                     AQVVWGLGYTFTSGAKQAWITDEIGEANANQLFLRAARFGSLAWLFGLGVTFLIGADN
                     VAIPIRGGAIGIILTGIALAFIMPENGFHPTPKEDRNTWQHMWHIFKEGLGAVRSQPR
                     LVNIVFIGLFYGLYSEGFDRLRVKHLLETFELPILFGSNQLAFFVVLDVIGTIISMFV
                     IRFVEKRIHVGNPIAIGRAMLFVTASITTAMIGFALSPVLGLSILSMLAVDVLRGVSG
                     PLQSTWINHKLDSRVRATIHSMFGQVDAIGQMTSGPTIGLIANALSVKLAISFSALLL
                     TPALFFTRRANSQAVNEMEVEPAPAD"
     gene            complement(1626..2957)
                     /locus_tag="VFQ23_12970"
     CDS             complement(1626..2957)
                     /locus_tag="VFQ23_12970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907555.1"
                     /translation="MVAETKKIIRDLGDGLILRHASSEDADSLSQFNGEIHAENEVDK
                     PYLMAWTRDLLTRPHPTLKPEDFTIIEEASTGRIVSSLNLIPQTWSYDGIQFGVGRPE
                     LVGTLQEYRGRGLVRIQFDEIHRWCEERGYRVQAITGIPYFYRQFGYEMAIDLDGRRY
                     GFESHVPTLKDGEEETYHVRLARESDLPFIAKTYDEIRKRNLLVCERTPEVFQYELSR
                     QEIEHSFAQTCVIEDTMGALIGYLKHLCFVSHGRLACIFYELKPDASWLDVTPSVVRY
                     LWQTGQAYAQRDGRVCNSFGFAMGEAHPVYEVIKDRLPDVRGSYAWYVRLPDLPGFLH
                     HIKPALEKRLTESIARGCSREIKISFYRSGLRIVVEKGHLTTIESWKPAPKDEGDIAF
                     PNLTFLQILFGYRSYEELHYAFPDCWCDSEEARVVINSLFPKRHSQIFPIA"
     gene            complement(3108..3704)
                     /locus_tag="VFQ23_12975"
     CDS             complement(3108..3704)
                     /locus_tag="VFQ23_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET9907556.1"
                     /translation="MSIKAVFFDLGGVIVRTEYQAPRQHLAERLGMEYDDLVKIVFDS
                     ESGYQASIGAVTPATHWESILKRLKRPAAELESIRHEFFAGDVIDRTILDFLRSLRGK
                     YKTGLISNAWGDLREYLLREKMDDAFEHMVISAEVGVVKPAAKIFQIALEQAGVKPKE
                     MVFVDDFAVNIEGCEKLGIKGIHFKDPVTAMKQLRALL"
BASE COUNT          979 a         1000 c          892 g          905 t
ORIGIN      
        1 tggagttcat gggcaaccca tgaactccac tgcctgctta ctcaaattgt tttgtcaatc
       61 agcaggagct ggttccacct ccatttcatt cacagcttgt gagttcgcgc gtcttgtaaa
      121 gaacaacgca ggtgtcagga gcaatgcaga aaatgagatt gccaatttaa cggagagggc
      181 gttcgcaatg agtccgatag taggtccact tgtcatttga ccaatggcat ctacctgtcc
      241 gaacatggaa tgaatcgtgg cgcggactcg cgaatccaat ttgtggttaa tccatgtgga
      301 ttgtaacggt ccactgacac cgcgtagtac gtcgacggcg agcatggaca aaatagaaag
      361 accaagaaca ggtgagagtg caaaaccaat catcgccgtt gtgatggagg cagtgacaaa
      421 caacattgca cgcccaatcg caatcgggtt ccccacatga atgcgctttt ccacaaaacg
      481 aatcacgaac attgaaatga tcgtcccgat cacatccaac acgacgaaaa atgccagctg
      541 gttactccca aacaggatcg gcaattcgaa ggtttcaagc aggtgcttca ctcgtaagcg
      601 atcaaaacct tcactgtata gaccataaaa caggccgatg aacacaatgt tgaccagtcg
      661 aggttgtgaa cgcacggcac ctaacccctc tttgaagatg tgccacatgt gctgccaggt
      721 attccggtct tccttcggag tcggatgaaa gccgttttct ggcatgatga acgccagtgc
      781 aattccggtc aatatgattc cgatcgcacc acctcgaatc gggatcgcga cattgtccgc
      841 gccgatcagg aacgtcacac caagcccaaa gagccatgcc agcgagccaa agcgcgcagc
      901 gcgcaggaat aactgatttg cattcgcctc gccgatttca tctgtgatcc aggcttgctt
      961 ggcaccactg gtaaatgtat acccgagccc ccagacaacc tgcgcaagga caattggcca
     1021 aaaagccggg aagaagcctt cgatcaggaa gccaagtccg atgaggatat agccaatgat
     1081 gatcgagaga cgccgggaat acgcgtctgc tacaacaccg gtgggcacct cgaacacaaa
     1141 ggcggaaacc tccagcgttg taccgatcaa tacaagttgt agtggtgtaa gcccggcaat
     1201 cgtgacctgg tatagcgagt tagtcacaaa aaccatacac gtgaagaccg cgagggccat
     1261 ctccatgaag agatagaccc gcgtagcgtc taactttaat ttgagaaaca tatatacctt
     1321 tgtagagtgg gcgcaggtcg ggttgagaac ccgaccgacc ataaaccaag ccgtaatcct
     1381 ctacaaggat tgaaggtaaa gaattgcggc agcgtcaggc aggagttgat tcttccatct
     1441 gccaggactt tgaagttgag aatagagttt tcattttttc tccagaaccc acccatccat
     1501 ccttgtcctc tccaaattcg actggcgttc gccggatttg ggaaggatgt cttgcaccgc
     1561 acgtgattta tgaaatgcgt acgcatttca tggcaaatca tgcaggtgag gacgggtggg
     1621 gtaggttatg caattggaaa tatttgcgag tgcctcttgg gaaagaggct gttgatcacc
     1681 acgcgtgcct cttcactatc gcaccagcaa tcgggaaagg cgtaatgcag ctcttcataa
     1741 ctgcgatacc cgaagagaat ttgcaggaag gttaaatttg gaaatgcaat atctccctca
     1801 tcttttggag ctggcttcca cgattcaatg gtcgtcaggt gacctttctc gaccacaatg
     1861 cgcaatccac tgcgatagaa actgatcttg atctcacggc tacatcctct cgcgatcgat
     1921 tcggtcagcc gtttctcgag agcaggtttg atgtgatgga ggaaaccagg caaatcaggt
     1981 aagcgcacat accacgcgta ggagccgcgt acatcaggca gacggtcttt tatcacctca
     2041 tagacaggat gtgcctcacc cattgcgaaa ccaaatgagt tacagactct gccatcgcgt
     2101 tgcgcgtagg cctgccccgt ttgccagaga tagcgaacca ccgatggggt cacatccagc
     2161 caggaggcat caggcttcaa ttcatagaaa atgcaggcga gccttccgtg actcacaaaa
     2221 cataaatgtt tgagataacc tatcagcgcg cccattgtat cttcgatgac acacgtctga
     2281 gcaaaggagt gttcgatctc ctgcctgctc agttcatatt ggaaaacttc cggtgtgcgt
     2341 tcacaaacga gcaggttccg ctttcgaatc tcgtcatacg ttttggcaat gaatggcagg
     2401 tccgattcgc gcgccaggcg aacatgataa gtctcttcct caccatcctt caatgttggc
     2461 acatgggact caaacccata tcttcgccca tccagatcga tcgccatctc atagccgaac
     2521 tggcgataga aatatggaat acctgtgatg gcttgcaccc tgtatccacg ttcttcacac
     2581 cagcgatgga tctcatcgaa ttgaatccgc acgagtccgc ggccgcgata ttcctgtaag
     2641 gtgcccacga gttccgggcg ccccacccca aattggatac cgtcatagct ccacgtctgt
     2701 ggaatcaaat tcaatgatga aacgatgcgc cccgtcgagg cttcttcaat aatcgtgaag
     2761 tcttccggtt tgagcgtcgg gtgtggacgt gtgagcaagt cgcgcgtcca tgccatgagg
     2821 taaggtttat ctacctcatt ctcagcatga atctcaccat tgaactgcga caatgaatct
     2881 gcatcttcag atgaagcatg acgcaggatc aatccgtcgc ccaggtcacg tatgattttt
     2941 tttgtttctg ctaccatgtg ttgcctttac acagcgccgc ataaatgagt caacttcaag
     3001 attcgattcc agtcgacaaa tccgtctaaa aagtttactt acgcatagac catgcgtgag
     3061 acaagcggca ggtatcctac aaccgccctt aaacgacaga gcagttttta taaaagggct
     3121 ctcaattgtt tcatggcagt cacgggatcc ttaaaatgga tgcccttgat accaagcttt
     3181 tcacagcctt cgatgttgac cgcgaagtca tccacgaaca ccatttcttt cggcttcact
     3241 ccggcctgtt ccagcgcaat ttggaagatc ttcgctgccg gcttgaccac tcctacctct
     3301 gcagagatga ccatatgctc aaaggcatcg tccatcttct ctcgcaagag gtattctctt
     3361 aaatcgcccc atgcgttcga gatgagtccg gttttatatt tgccgcgcag ggaacgcaga
     3421 aaatccagaa tggttcgatc aatcacgtca cccgcaaaga attcatgacg gatcgactcc
     3481 aattccgctg ccggacgctt caaccttttt aagattgact cccaatgtgt agctggtgtg
     3541 actgcaccaa tcgaggcctg ataccccgat tcgctgtcga agacgatctt gacgaggtca
     3601 tcgtactcca tacccagccg ctccgcgaga tgctgacgcg gcgcctgata ttcggtgcga
     3661 acgataaccc cgcccaaatc gaaaaagact gcctttatgc tcatttattt ctcttttttc
     3721 cctgcgaggg tgtatttttc gccggtggtt ttctgccttt ctcagctctt gcagca
//
LOCUS       DASTJP010000449        11478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_328587, whole genome shotgun sequence.
ACCESSION   DASTJP010000449 DASTJP010000000
VERSION     DASTJP010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11478
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328587"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFQ23_12980"
     CDS             <1..989
                     /locus_tag="VFQ23_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9907557.1"
                     /translation="ESNLPIDLLENPQASSGEILMKAIVCTRYGPPEVLQLKEVKKPM
                     PRKNEVRIKIFATAVTASDCLIRGLKFRPRARLLIRLLFGVKAPRQPILGMVLAGEID
                     SVGRDVSAFKPGDPVFGISERRYGTYAQFVCWPASGLLAVPPANLTFEEAAALPYGGL
                     LALYFIRKAEIKAGQKVLVYGASGAIGTAAVQLIRHLGANVTGVCSTANLQLVESLGA
                     DAVIDYTREDFTSRPERYDVIFDAVGRRKSRGALADSHLALLPNGKVVSVDDESPKMT
                     AADLVILQELAESGTLKPVIDRIYPLEETAEAHRYVDGGHKKGNVIITVTHP"
     gene            complement(1302..3143)
                     /locus_tag="VFQ23_12985"
     CDS             complement(1302..3143)
                     /locus_tag="VFQ23_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907558.1"
                     /translation="MKTWFIRFRFLVMCIVILSLLIPQGTVHAWKPRTHVFLAEIALQ
                     DALDDGMLTIYATDYANGRVLNDLGGKPRIIGSYPVDPVILTALKSNAAQFRAGVLGP
                     DAYPDIATGQQIIHPAGKDTPGQPSGVDINAGGPGSGPWLEYLYSRAFTGCNPDGSAC
                     NAADKTPANQAFVMGFLAHAAGDMYGHTFINYYTGGPFHFSPKTENAIKHIVLEGYIE
                     KRTPQISYDANIDAGVDQFIYRNMIKAAPNSYLVQKLLTGTSTEYSLPYIYSQLRNQL
                     QKDIDAYYATKKNYDKQAAEIQAKINACGWLDWSCSNIALGAQLSWIQTQKGYYVTQN
                     AAQITYKEAWRDDIDQGLAALPQMSHKLAKALFFNPNEMDKAQAQAIIEDYVLNHLTS
                     MGGAPDFAGQSIYWVKGVITQVLNALGITWLKDAIDQAERDLLNYLMMNALGFTVDDI
                     EKYLKDPATQFDPTLNSSTFNVEGTGHLISLQSFNADQLHITDTGNNNPAERFDYQKF
                     PAAFNTVVMIKLSLMSQAGIRQLMTDLGDRGVQGVQPPGAVIKPNILLGYIETLDGGN
                     QWIVNGHSSPGVAYLSLEMIFARSCQLYQKIFMQQVGASDLMSACQL"
     gene            3641..4237
                     /locus_tag="VFQ23_12990"
     CDS             3641..4237
                     /locus_tag="VFQ23_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907559.1"
                     /translation="MNRVREEKGFRTIDEIYELAKENTIFNPFSVLISRSVSLGKGNI
                     IYPNVVIENMAGIIVIGDHNNLFNGTTIRNLSGKIEISNDNEIGENAISIKNSQGKIE
                     IKDNCRLMNGAQILDDCYVGNGAQVLGNIKMRGCILADGESYKEKNPNRRGGVLKGFG
                     TANNLQIEIGMVINGNGSMSADMIERQETYHPNWAEEK"
     gene            complement(4272..4928)
                     /locus_tag="VFQ23_12995"
     CDS             complement(4272..4928)
                     /locus_tag="VFQ23_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9907560.1"
                     /translation="MTPIRLMIADDHTLFREGLKALLAVTDDIDIVAEAEDGDSALKK
                     CKELQPDVILMDINMPVLNGLQVTQQIVEAFPQIGIIMLTMLEDDASVFHAMRAGARG
                     YLLKGAHPQEVLSVIRAVSEGQALFGSAIATRLMNFFRELSSKPVAAQSKVNFPELTE
                     RELEVLRLISRGLNNAEIAQKLVLSPKTVRNHITNIFSKLQVADRAQAIVRAREEGVQ
                     "
     gene            complement(4925..7252)
                     /locus_tag="VFQ23_13000"
     CDS             complement(4925..7252)
                     /locus_tag="VFQ23_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9907561.1"
                     /translation="MEQVSAPSLINTKLEKPWLLPLRLLWLAASLIALGLFVAGLPLR
                     AREIHNWYGGNIEAGLTENQAGEVVVSTWPGSASAQAGVLQDDVLLAVDGVPVVSLEQ
                     AKTLTEGVIGSQVTVSVRTGNFPPRQLMVTRGSLAGEVLLNYGLSSQFAVGFVLTSEI
                     LFALLCVGIAGVIFWYRPDDWMALFSALIIIVILVGLSLPVLALADSLQNGGYPLWID
                     TWYALGFGSLILFFYLFPVGRFTSYVTFGLAAVLGLWLTLGLFNRSLLLWYQPRLTFV
                     SLGLAWVASGVASLVLRYRQSESVQRQQILWIVWGASASAVGLMLQIFPTLFELSPST
                     RLLYDFVLYPLGQILKACLPLSIGFAILRYRLWNIELVLNRLLVYGSLSVLTMLGYLG
                     AVFILQALFTGLSNPITSFFATGVVAVLFEPLRRRLQRAVNRMMYGERDDPYVVLTRL
                     AGTLEHISTTNEVLPSIAQTIGQTLKIPYVAILLDQDGAERLAASFGEPVHELLSFPL
                     VYHSEVIGRLQLARRAPNEQFLNTDLGLIESIAHQAGAAAQTVRLNAELIRSRTQIVN
                     EREDERLRIRRDLHDELGPMLASQSFKLAAARQVIRTKPETAESLVDEIIQQSQQTIA
                     DVRRLVHGLRPPALDQLGLVEAVRDLVRNEADGLNFDVSAPTGGLPSLPAAVEVNAYR
                     IVLEALNNAVKHARASHCIVRFSSPMTDSLVIHIEDDGVGMPKEYRPGVGLRSMRARA
                     EEIGGELHIESLSPRGTQLVARLPLVSAQEREYVK"
     gene            complement(7297..8571)
                     /locus_tag="VFQ23_13005"
     CDS             complement(7297..8571)
                     /locus_tag="VFQ23_13005"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9907562.1"
                     /translation="MKARHVLFVLLVVSLSACNAFAPAATPTPIPLPPPQHAESAFQW
                     LETHAVLKDNVDWATLRSDTADIITNAKTTTDTLPAICLAIRNLKDGNAWVLVPGLET
                     PNFYTGYQTLYPENNVIIRVIPDSPAEKAGLQVGDLIEQVNGRAPIPYEEMDAFPPCN
                     AEEIDTATEEHLVINRNGGSIQMTVERVTLTAGVDPYLPATGERFGADASGIGYIELN
                     TESGMHFEFATDVQKLMRGMDKTPVCGWMIDLRRNLGGDIWSYIAAVGPILGEGDLGG
                     FEYLDGTREGWTYRNGQVLWNNEYRDESEIDGAIYTPKQVTPVALLTSPATQAAGELM
                     VVAFEGRPAVQSFGEPTRGLPTLVTHTELSDGSALYVSGANSFDRNGTIYSGPIAPDV
                     FIETDWSKFGTEQDPVLLAAMDWLHSQSACVP"
     gene            complement(8591..8899)
                     /locus_tag="VFQ23_13010"
     CDS             complement(8591..8899)
                     /locus_tag="VFQ23_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907563.1"
                     /translation="MNIHFQQVEKKSQMFSYILAAFAVFTIVMLALSFSRHEKLHLRM
                     PSESVQVIHSENISLPVAIPAPLPPAEQTQSGATPEPSGNSQTALVPQAIAAPMPSVP
                     "
     gene            9161..9874
                     /locus_tag="VFQ23_13015"
     CDS             9161..9874
                     /locus_tag="VFQ23_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907564.1"
                     /translation="MTNPSPLLYNTYYHIYNRGVNGENLFREERNYDLFLRLYERHLS
                     PVTDLFAYCLLRNHFHISVRVKSEEEIIETKKTQHKETLKVSAINMRHNRVKSLPNEQ
                     IGKPGRPLGSGSLSSRYVSDQFSNFFNAYAKTINKAYGRTGSLFEHPFERVCITTEKQ
                     FWNVLAYIHQNPQKHGFVKDFRDWKYSSYGLILSRKPAIINREEVLKWFGTREDYLSL
                     HNQWVTDAQSKWIIEKDLD"
     gene            complement(10046..10549)
                     /locus_tag="VFQ23_13020"
     CDS             complement(10046..10549)
                     /locus_tag="VFQ23_13020"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9907565.1"
                     /translation="MSNVTLPKEDAVVIERTFNAGVKLIWQMWTDPEHFKHWYGPTGF
                     TVPVADMELRVGGKRLICMASPDGRMKMWTVGEYKEIIPNVRLVYTESPADENGNVVS
                     PSAMGMPDGYPTTTEVTVLLEDLGGRTRMLMKHAGVPAESGANRGWEQAFEKLVEHIE
                     TTLKNNS"
     gene            complement(10546..10893)
                     /locus_tag="VFQ23_13025"
     CDS             complement(10546..10893)
                     /locus_tag="VFQ23_13025"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9907566.1"
                     /translation="MMRTSNQKLDLTFAALANSTRRAILDRLAKGEATVNELAAPFNM
                     SLPAISKHIKVLERAGLITQGQKAQYRPCAIDAAPLQEISRWTEQYRHIWETRFDQMD
                     DYIHQLNQKGDTQ"
     gene            11298..>11478
                     /locus_tag="VFQ23_13030"
     CDS             11298..>11478
                     /locus_tag="VFQ23_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907567.1"
                     /translation="MGKVIFEISMSLDGFIAGPRDDSNPDRELGGLDFLHDWRFGNQS
                     PGEAEAYELEKFKPMG"
BASE COUNT         2936 a         2914 c         2888 g         2740 t
ORIGIN      
        1 cagaaagcaa cttgccaata gacttgcttg aaaatccaca agcatcttcc ggagaaatcc
       61 ttatgaaagc aatcgtatgc acacgttatg gtccccctga agttcttcag ctcaaagagg
      121 tgaagaaacc gatgcctcga aagaacgaag tacgcatcaa aatttttgcc accgctgtca
      181 cagccagtga ctgcctcata cgaggcttaa agtttcgccc ccgggcccgc ctcctgattc
      241 gattgttgtt cggagtcaaa gcgccgcgcc agcccatcct tggcatggtg ctcgccggag
      301 agatcgactc agtcgggcga gacgtatcag cgttcaagcc tggtgacccg gtgttcggca
      361 tcagcgaacg ccgatacggt acctacgcgc agtttgtttg ctggcccgcc agcggattgc
      421 tcgcggtgcc acctgcgaac ctgacctttg aggaagcggc tgcactcccc tacggcggtc
      481 ttctggcgct ttacttcatc cggaaagcag agatcaaggc tgggcagaag gtgctggtat
      541 atggcgcgtc gggcgccatc ggcacggccg ccgtgcagct gattcgacac ctcggcgcaa
      601 atgtgaccgg ggtgtgcagc accgccaacc tccagctggt cgaatcactc ggagccgatg
      661 cggtcattga ttacacacgc gaggatttca cgagtcgacc agaacgctat gacgtcatct
      721 tcgatgccgt gggacggcgc aagagcagag gcgctctcgc agactcccac ctcgcattgc
      781 tgccaaatgg caaagtcgtc tcggtggacg atgaatctcc aaaaatgacc gcagcggatc
      841 tcgtcatcct ccaggagctg gcggagtcag gtaccttgaa gccggtgatc gatcggatct
      901 atccgctgga agagacggcc gaggcgcacc gctacgtgga tggcgggcac aaaaagggga
      961 atgtgatcat tactgtgaca catccctgat ttcatttact caccgaaggc ctggcctggc
     1021 aaaattacta tccggacacg ctggagaaca ccgctgctca ttcaaattcc gtacgccagc
     1081 gcttccccag ccggccgaat cgtgccgaaa gatggagcag gatttcctat tggacgtagg
     1141 caatggaacc aagcctgaat tcgaaaacaa attttaacgc ggcgagaact ccgtcattat
     1201 ttatccactc aatacggaac cctttcaagg tttaagcagg ggtttgagcc ctgaggtcca
     1261 cagccaaacc agaaaactta cacctgctga tctcccaacg gtcataattg acatgctgac
     1321 attagatcag aagctcctac ttgttgcatg aaaatcttct ggtagagctg acaacttctt
     1381 gcaaatatca tttccaagct aaggtaagct acgccgggac ttgagtggcc gttgacaatc
     1441 cattggttac cgccatccag agtttcaata taacctagca ggatattggg cttaatcaca
     1501 gcccctgggg gttgaacccc ttggactcct ctatcgccca gatcagtcat gagttggcga
     1561 attcccgctt gactcatcaa cgacaacttt atcatgacga ctgtgttaaa cgcggcaggg
     1621 aatttttgat aatcaaaacg ctcagccggg ttattgttgc ctgtgtcagt gatgtgcagc
     1681 tgatctgcat tgaacgactg gagactaatg aggtgccctg ttccctctac gttaaaggta
     1741 gagctgttga gtgtgggatc aaattgcgtc gccgggtctt tgagatattt ctcaatatca
     1801 tcaactgtaa atcccagtgc attcatcata aggtaattga gcagatcacg ctccgcttga
     1861 tcaatagcat ccttgagcca tgtaataccc agggcattga gcacttgcgt tatcactccc
     1921 tttacccaat agatcgactg gccagcaaaa tctggagcac ctcccataga ggtcagatgg
     1981 tttaagacat aatcctctat gattgcctgc gcttgagctt tatccatctc gtttgggttg
     2041 aagaacaatg cctttgcaag cttatggctc atttggggca atgcagctag accctgatcg
     2101 atatcatcgc gccaggcttc tttgtaagta atctgtgcag cgttctgggt cacataatat
     2161 cccttctgag tctgaatcca ggatagctgg gcgcccaacg ctatgttgct gcaggaccag
     2221 tccaaccaac cacatgcatt tatctttgct tgtatttctg cagcctgttt atcatagttc
     2281 ttttttgtgg catagtatgc atcgatatct ttctgcagct gatttcggag ttgtgagtag
     2341 atgtaaggca gcgaatattc tgtactggtg ccggtcagca atttttgtac cagatacgag
     2401 tttggtgcgg ccttgatcat gttccggtag atgaactgat ctacgccagc atcaatgttc
     2461 gcgtcataag agatttgcgg tgttcgcttc tcgatatatc cttcgagtac aatgtgcttg
     2521 atcgcatttt ccgttttggg tgagaagtga aagggcccgc cagtataata gtttatgaat
     2581 gtgtgtccat acatatctcc tgcagcatga gcaaggaatc ccatgacaaa cgcctggttt
     2641 gccggagtct tgtcggcggc attgcaggct gaaccatcag ggttacaacc ggtgaaggcg
     2701 cggctataga gatattctaa ccaggggcct gatccgggtc ctccggcatt aatatccact
     2761 ccgctcggtt gaccgggcgt atctttcccc gccggatgga tgatttgttg acccgtggca
     2821 atatccggat aggcatccgg gccgagcact cctgccctaa attgtgccgc attggatttt
     2881 agcgctgtca gtatcaccgg atcaactgga tacgaaccga ttatcctggg cttaccaccc
     2941 aagtcgttga gcacacgacc gttggcgtaa tctgttgcat agatcgtaag catcccatcg
     3001 tcaagcgcat cttgcagagc gatctctgcc agaaaaacat gggtcctggg cttccaggcg
     3061 tgtacagtcc cctgagggat caggagagac aaaatcacaa tacacatgac aagaaaccta
     3121 aacctgataa accaagtctt cattgttgcc tccatgaaat aggttatccc caacgggaat
     3181 acgcttcccg ttgatcttgt tagcatgtat ttaatgtacg atcagcagtg agaattattc
     3241 ggcttcccca caggaaacct atcctacctc tctttcctgg gtccacttgt tcgaccttac
     3301 ttaacggacc agagaattgg taactggatt atagactaat gaggattgat gtcaaggttt
     3361 tttgtaatct attcgacttg agaactgcct ggaaggagtc aaataagatt gttgtgttaa
     3421 taaaggcgtc tagcggaata acgaacaaaa caaactacgc ttctgtccca actcgttgct
     3481 ccaaaaccag gtatccctat cctcggccgg cgtagccgtg ccgaggatgg ggagctttct
     3541 ctctgttgat agagaagagc acgtataccg cgaatgctat aataatttca gagccagtct
     3601 ttggagtttg cacatggatg tagagcagta tattaaaaac atgaacaggg taagggaaga
     3661 aaaaggattt cgaacgattg acgagatcta tgaactggca aaagaaaata cgattttcaa
     3721 tccgttttct gtgctcatta gcagatctgt ctctctgggg aaagggaata ttatttatcc
     3781 caatgtagtg attgaaaata tggctggaat aattgtgatt ggagatcaca acaacctttt
     3841 caatggaacg acgatacgaa atctttctgg caaaattgaa ataagcaatg acaacgaaat
     3901 tggtgaaaat gccatcagta tcaaaaacag ccagggcaaa atagagatca aagacaactg
     3961 caggctaatg aacggggctc aaattttgga tgactgttat gtagggaatg gagcgcaagt
     4021 attggggaac ataaaaatga gaggctgtat cctggcggat ggggaatcgt ataaggagaa
     4081 aaacccgaac aggcgcggcg gtgtgttaaa aggatttgga acagccaata acctgcagat
     4141 tgaaatagga atggtgatca atgggaatgg cagtatgagc gcggatatga tcgaaaggca
     4201 ggaaacatat cacccaaact gggcggaaga aaaataaagg ggaatagcta aaagcctgca
     4261 atagatgaac ctcattgcac gccctcttcc cttgcgcgga caattgcctg ggcacgatca
     4321 gctacctgaa gtttgctgaa aatgtttgtg atgtggtttc gcacggtttt cgggctgagc
     4381 accagcttct gcgcaatctc cgcgttattc agccctcgcg agatgaggcg gagaacttcc
     4441 agctcacgtt ccgtcagttc ggggaagtta actttgcttt gagcagcaac cggttttgaa
     4501 ctcagctccc tgaagaagtt catcagccgg gtagcaatcg ccgaaccaaa caaagcttgg
     4561 ccttcagaca cggcacggat cacgcttaag acttcctgcg gatgggcacc cttcaagaga
     4621 taccctcgag ctcccgcgcg catggcatgg aaaacggaag catcgtcctc cagcatcgtg
     4681 agcatgatga tgccgatctg cgggaacgct tctacgatct gctgggtgac ctgcagacca
     4741 tttagcacgg gcatgttgat gtccatcaga atgacatccg gctgcagttc tttacatttc
     4801 ttcaatgcgc tatcgccatc ttctgcctca gcaacaatgt caatatcatc tgtgacagcc
     4861 agcaaggctt tcagaccttc gcgaaatagt gtgtggtcgt ctgcgatcat caatctgata
     4921 ggagtcattt aacgtactct ctttcttgtg cggagactaa cggcaatcgg gcaacgagtt
     4981 gtgtgccacg gggcgaaagc gattcaatat gcaattcccc tccgatttct tccgcccgcg
     5041 ctcgcatgga tcgtagtccc actcccgggc gatattcctt tggcattcca acaccatcgt
     5101 cttcaatgtg gatcactaac gaatcggtca tagggctact aaatcgtaca atgcaatggc
     5161 tggcgcgggc gtgcttcact gcgttgttca aagcttctaa aacgatccga taggcattga
     5221 cctccacggc ggcgggcagg gaaggcaagc cgcctgtggg cgcagaaaca tcaaaattga
     5281 gtccgtcagc ttcgtttcgc acaaggtcgc ggacggcttc cactaatccc aattgatcca
     5341 atgccggcgg acgtaatcca tgcaccaatc gtcgcacatc ggcgatggtc tgctggcttt
     5401 gttggataat ttcatcgaca agactctctg ccgtttctgg cttggtgcga atgacctggc
     5461 gggcggctgc gagtttgaag ctctgactgg caagcatggg acccaattca tcgtgcaggt
     5521 ctcggcggat tcgcagacgt tcgtcttcgc gctcgttgac aatctgcgtg cgagagcgaa
     5581 ttaattctgc attgagccga acggtttgcg cggcggcacc cgcctgatgc gcaatactct
     5641 cgatcagccc caggtcggta ttgaggaatt gttcattggg ggcccggcgg gcaagttgta
     5701 atcgtccgat cacttcgctg tgataaacca gtggaaacga aagaagctca tgcaccggct
     5761 cgccaaagga agccgccaat cgctctgccc catcctggtc cagcaggatt gccacatacg
     5821 gaattttgag cgtctgcccg atggtttgcg caatggacgg caatacttcg ttcgtcgtgg
     5881 aaatgtgctc gagggtccct gccaggcgtg tcagcaccac atacggatca tcgcgttcgc
     5941 catacatcat ccggttgacc gcacgctgca atcgccgtcg cagaggttca aacaaaaccg
     6001 caacgacccc ggtcgcgaaa aaggaagtga tagggttgga taagccagtg aagagtgcct
     6061 gcagaataaa gaccgcaccc agataaccca gcatcgtcaa cacagaaaga gaaccgtaca
     6121 ccagcaggcg gttgaggacg agttcgatgt tccacagtcg ataacgcagg atggcaaagc
     6181 caattgacaa tggcagacag gctttgagaa tttgccccag tgggtacagc acaaagtcgt
     6241 aaagcaggcg cgtggaagga gagagctcga atagtgtcgg gaatatctga agcatcagac
     6301 ccaccgcgct ggcggaagct ccccatacga tccagaggat ttgctggcgt tgaacagact
     6361 cagattggcg ataccgaaga accagcgaag cgactccgct cgccacccag gccaagccta
     6421 atgagacaaa ggtcaatcgc ggttggtacc acagcagcag cgaccgattg aaaagcccca
     6481 gcgtgagcca gagccccaga acagcagcca gtccgaacgt cacgtatgac gtgaagcgtc
     6541 caacagggaa aagataaaag aacaggatca acgatccaaa gccaagtgca taccaggtgt
     6601 cgatccatag cggatatccc ccgttttgca ggctatccgc gagcgccaat acgggaagtg
     6661 ataatccgac caggatcacg atgatgatga gcgctgagaa gagtgccatc cagtcatcag
     6721 ggcgatacca aaagatcacg ccagcgatcc caacgcaaag gagagcaaaa aggatctcgc
     6781 tggtcaacac aaagcctaca gcgaattgac tggagagtcc atagttcaac agaacctcgc
     6841 ctgccaaact tccacgcgtg accatgagct ggcgcggggg aaaattacct gtccgcacgc
     6901 tgacagtcac ctgcgagccg atcacgcctt cggtaagagt ttttgcctgt tccaacgaaa
     6961 cgactggaac gccatctact gccagcaaaa catcatcttg aagcacaccc gcttgtgcag
     7021 aagcggagcc tggccaggtc gatacaacca cctctccagc ctggttttcc gtcagcccag
     7081 cctcaatatt cccaccgtac caattgtgga tctcccgggc acgcaaaggc aaaccggcaa
     7141 cgaacagccc cagcgcgatc agagatgcag ccagccaaag gaggcgcaga ggaagaagcc
     7201 agggcttttc aagtttcgtg ttgatcaaag aaggagcgga tacttgttcc atatttacct
     7261 tttgtgggtg tcacgtagct gcattggcaa atcgcgctag ggcacgcagg cagattgaga
     7321 gtgaagccaa tccatcgctg ccagcaggac gggatcctgc tcggttccaa acttcgacca
     7381 atccgtctct ataaacacat ccggagcgat cgggccactg taaatcgtcc cgttgcgatc
     7441 gaaagaattt gctccgctca catacaacgc ggagccatcg ctgagctcgg tatgcgtgac
     7501 gagggttggt agtccgcgcg tgggttcacc aaaggactgc acagctgggc gtccctcaaa
     7561 ggcgacgacc atgagttcac ctgccgcctg tgtcgcagga ctggtcaata acgcaactgg
     7621 tgtcacttgt ttcggagtat agatcgcgcc atcgatctcg ctctcgtcac gatactcatt
     7681 attccacaac acttgaccgt tgcggtaggt ccagccttcc cgcgtgccat cgaggtattc
     7741 aaacccaccg aggtcgccct cccccaggat cggcccgacc gctgcaatat atgaccagat
     7801 atctccaccc aggtttcgtc gcagatcaat catccagccg cagacgggcg ttttatccat
     7861 gcccctcatt aatttctgga cgtccgttgc aaattcaaaa tgcatgccac tttccgtgtt
     7921 caattcaatg taaccgatgc cgctcgcatc tgcaccaaag cgctcacctg ttgcaggaag
     7981 ataagggtca acccccgcag tcaatgtgac cctttcaaca gtcatctgta tggatccgcc
     8041 atttcggttg atcaccaaat gctcttcggt ggcagtatcg atctcctccg cgttgcacgg
     8101 tggaaaagca tccatctctt cataaggaat gggtgcacga ccattgacct gctcgatgag
     8161 gtctcccacc tggagccctg ccttttctgc cgggctgtct ggaatgaccc ggatgatcac
     8221 gttgttttca ggatagagcg tctgataacc ggtataaaaa ttgggcgtct ccagccccgg
     8281 gactagtacc caggcgttgc catccttcaa gttgcggatt gcaaggcaaa tggcaggaag
     8341 ggtatctgtc gttgttttgg cattcgtaat gatgtcggct gtatcactgc gcagcgtagc
     8401 ccaatccacg ttatctttca gcacggcgtg ggtctccagc cattggaacg cgctttcggc
     8461 atgttgcggc ggtggcagag gaattggcgt tggggtcgct gcgggagcga acgcgttaca
     8521 ggcagataaa ctcactacca gcaaaacgaa aagtacatga cgtgctttca tttccatctc
     8581 ctatcatcta ttatggcaca gaaggcatcg gtgcggcaat agcctggggc accagggcag
     8641 tttgagaatt cccggacggt tcgggtgtgg caccgctttg ggtctgttcg gcgggtggca
     8701 atggggcagg gatagcgaca ggcaaactga tattttctga atggataact tgcaccgatt
     8761 cggacggcat ccgcaggtga agtttctcat gacggctgaa gctgagggct agcatcacga
     8821 tcgtgaaaac tgcgaatgca gccaggatat acgaaaacat ttgtgacttc ttttcgacct
     8881 gctgaaaatg gatgttcatg gctacctctc aatgtatcgt ttttgtgaga ctgatacaga
     8941 tactatacaa gtcagcctgt cctgttgtca tgagacccat gtcccaaacc agtcctgctt
     9001 ttggctggga ctgacgtctt attttgccat cgaacaaaag aggacttagt ggcataacta
     9061 agtcctcttt cctctgaacg gctacgaaga gtctcggttt tgaaattgaa aagaatcact
     9121 aaggatgctt acgcccctca acatgtagaa tttaaatatc atgaccaatc cctcgccttt
     9181 gctctacaac acctactatc acatctacaa tcgcggcgtg aacggcgaaa atctattcag
     9241 agaagaacgg aattatgacc tttttctgag gttatatgaa aggcatcttt caccagttac
     9301 tgacttgttc gcatattgtc tgttgagaaa tcacttccat atttcagtgc gagtgaaatc
     9361 ggaagaggaa ataatagaaa cgaaaaagac ccaacataaa gagaccctaa aggtctctgc
     9421 cataaacatg cgtcataata gggtaaagag ccttccaaat gaacaaatag gtaagccagg
     9481 aagaccttta gggtctggga gtttaagttc cagatacgtc agcgatcaat tctccaactt
     9541 cttcaacgcc tacgccaaga cgatcaacaa agcctatgga cggaccggca gtttgttcga
     9601 gcaccccttc gagcgtgttt gcattaccac ggagaaacag ttctggaacg tgctcgctta
     9661 tattcaccag aaccctcaaa agcacggatt tgtgaaagac ttccgcgact ggaagtactc
     9721 atcctatggg ctcatcctga gcaggaaacc tgcgatcatc aatcgagaag aggtcctgaa
     9781 atggttcggg acaagggagg attacctatc gcttcacaac caatgggtga ctgatgccca
     9841 atccaagtgg atcatagaga aggatttgga ttgacattaa tttttttttg cagacccgtc
     9901 ataaaaatgt cagagacatg aatctcctcc caaaggactg tggcgaaacg actgtgcaaa
     9961 aattttcagg gcaacctttt ggtgaatcac cgtggttgcc ctgaaataat ctcaggtact
    10021 gtcaaaacga ccgaagcatc atgccttatg aattgttttt gagggtggtt tcgatatgtt
    10081 cgactagttt ttcgaaggcc tgttcccaac ctcggtttgc acctgattct gcaggtacac
    10141 cagcgtgttt catcaacatt cttgtacgtc cacctaggtc ttccaaaagc acggtgactt
    10201 cggtcgtggt gggatagccg tccggcatgc ccatcgcaga cggcgaaact acattgccgt
    10261 tttcatcagc cgggctttcg gtgtagacca ggcgcacatt aggaatgatt tccttgtatt
    10321 caccgaccgt ccacattttc atacggccat ccggcgaggc catacagatc aggcgtttcc
    10381 caccgacgcg cagctccata tcagcaaccg gcaccgtgaa gcctgttggt ccgtaccaat
    10441 gtttgaagtg ttcggggtcc gtccacattt gccagataag ctttacgccc gcattgaacg
    10501 tgcgttcgat cacaactgca tcttctttgg gtagggtcac attactcatt gggtatctcc
    10561 tttttgattg agctgatgga tatagtcatc catttggtcg aagcgcgtct cccaaatatg
    10621 gcggtattgt tctgtccacc tggaaatttc ctgcaggggc gctgcatcaa tggcacaggg
    10681 acgatattgc gctttttgac cctgcgtgat cagcccggcg cgttccaaaa ccttgatgtg
    10741 cttggaaata gcaggcagac tcatattaaa tggtgctgcc aattcgttca cggtcgcttc
    10801 cccttttgcc agacgatcca ggatcgcccg ccgcgtggag ttcgccaggg cagcaaacgt
    10861 caggtctaac ttttgattac tggtcctcat catattgaat ttccttattt taccgattgg
    10921 ttaattaacc tatcagtata atacaattat tggttccatt tgtcaagtgc acaaccctgc
    10981 gacaggcgta cgcgccttcg ctatgaatat ccctatccgc accgccctgc cgaaggatgg
    11041 gaagggcttc gatttatttt ccttctgacg gatgctttcc agggaagact cgcgcctgta
    11101 cggatttcat gtatatggca accagtcaaa agggaatgca ataatcctgc aaaaaagggg
    11161 agttttcgtc ccacgtgtga acgcgcgggc gaagctaaag accagttcgt tacataggac
    11221 tttgccgcag atttagaaag attacgattg gactatgagc gatcaaaaaa caataaggca
    11281 cttaaaagga gatcctcttg ggtaaagtta ttttcgaaat tagtatgtcg ctggatggtt
    11341 tcattgcggg accgagagat gacagcaatc ctgaccgcga attaggagga ctggatttcc
    11401 tccacgactg gaggttcggc aaccaatctc cgggggaggc ggaagcctat gaactggaaa
    11461 aattcaaacc aatgggag
//
LOCUS       DASTJP010000450         4588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_660097, whole genome shotgun sequence.
ACCESSION   DASTJP010000450 DASTJP010000000
VERSION     DASTJP010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660097"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..673)
                     /locus_tag="VFQ23_13035"
     CDS             complement(200..673)
                     /locus_tag="VFQ23_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9907568.1"
                     /translation="MNTKIALSPIIVQPGAGKDLHAFGDIMTVMLGGEQTGNQLSVLF
                     GMTPPGGGPPLHVHHREDEIFLVVEGRISYCVNGEWTEVGPGGVIYLPREVPHCYRNV
                     GETPSRHWIITTPSGFEIFFARCAEEFAKPGGPDMGRILEIINEHGGALLEGGQT"
     gene            815..3172
                     /locus_tag="VFQ23_13040"
     CDS             815..3172
                     /locus_tag="VFQ23_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9907569.1"
                     /translation="MEKHSFGYWLRLRRKALDLTQDALADRVGCSVGMIRKMESEERR
                     PSAQIAERLAQIFNIPPGEHLDFLRFARGDVKSTPVETEEGLPWRASGKSPSSNIPAT
                     TTSLIAREKEIAEVHAYLAQDDIRLVTLLGPPGIGKTRLSIESAHRAQPDFSDGAFFV
                     ALAPLDNAGLIAQTIAQSLGFVGATNLHVEEQLKQGIGKRHILIVLDNCEHLIEDVAR
                     LAYDLLSACPRLSILATSREVLRVPGEWLYAVPAFELPTERAALDVEGASTYPALTLF
                     SERARAVRSDFALSPENIQSIAAICAQLDGLPLAIELIAARIRLMSPQSLLERMSGHF
                     VLTADGMRIASGRQKTLRSAIDWSYNLLPPEEQKLFAYLSVFSGGFTLAAAEAIFSRS
                     FTEKSVPELLALLLDKSLVRRVSSESREDRYEMLVTVEEYAHERLQHWGEEAEVRNWH
                     LDYFLSLAEKADNELRGPNQPEWLNRLNVMHDNVRTALDWGIETGQTETALQLASRLW
                     WFWLMRSEFNEGRQWLGRVLAMPDVTSFPYLHAAILTQLANHTWLQSESDEARPIIEQ
                     ALPVARSLGDPQTLAYTLMVFALVLTSEEDFAAAQSTFEKSMTLFREGGDKWGYAMAV
                     MCLASVAHRNDDQVKALALNEQALAGFRELGETYFQSVCLWASGHLRAKLGDWKAGLE
                     ELRESLTLSRRLDSKLEVASVLLRLAEVEGHLEDPARAVRLYWAARNVYDSIGVRYTT
                     DNPNLEKRLVSCRTALGEAAYAEAMEQGRLMTREQAIAYALEDQE"
     gene            complement(3244..3729)
                     /locus_tag="VFQ23_13045"
     CDS             complement(3244..3729)
                     /locus_tag="VFQ23_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907570.1"
                     /translation="MKKLIVSTTLAPLLVVALAGTTLAAPAKAGKKELPFKGTLQSVE
                     TYAVTPPIMAVTANGSGNATHLGQYAISYEVEVNLETLAGVGSAQLVAANGDILYANM
                     SGQATPTGTTDVFNVVEEFTITGGTGRFANAGGSFTLNREVNITTGVTSGTMSGAIIL
                     P"
     gene            3890..>4588
                     /locus_tag="VFQ23_13050"
     CDS             3890..>4588
                     /locus_tag="VFQ23_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9907571.1"
                     /translation="MQAHSFGNWLKIRRKARDLTQAELANQVGCSAAAIRKLEAEERR
                     PSAQIVERLAEIFEIPQNEKANFLRFARGEMRSAPAETTEDSPWQVSGGLTRSNLPIT
                     VTSLIGREKEIAEVRNYLSKDDMRLVTLIGPPGIGKTRLGIESAGTLLPNFPEGVFFV
                     ALAPLEDPAMIAQAVAQSLGYVGTRNIPVTEQLREGIGRKQMLLMLDNCEHLIEDVAA
                     LASSLLSACSGLKIL"
BASE COUNT         1068 a         1191 c         1232 g         1097 t
ORIGIN      
        1 ttgaccgtca tatccaaatt gcgcgtgagc aactgggcgc agataaattt gaaaacctgg
       61 caagagaggg tcatgccatg acgatggaac aggccatcgc gtatgctctt gaagatcagg
      121 aatgaaatag tggtcggcta tgccgaccac tacccataat gctgcgatta aattggtgat
      181 attgctggtc cagcggttat caggtttgcc cgccttcaag caaggcaccg ccatgttcgt
      241 tgatgatttc gaggattcgc cccatgtctg gcccgccggg tttcgcgaac tcctccgcgc
      301 aacgagcgaa gaagatttca aatccagagg gggtcgtaat gatccaatgg cggctcggcg
      361 tctcgccaac gttacggtag caatgcggca cctcgcgcgg caggtagatc accccgcccg
      421 gtccgacttc tgtccattcg ccattcacac aataactgat gcgaccctca acgacgagaa
      481 atatttcgtc ttctctgtga tgcacatgca aaggtgggcc gccgcctgga ggcgtcatgc
      541 cgaacagaac cgagagttga ttgccggttt gttctccgcc aagcatcacc gtcatgatgt
      601 ctccaaaggc gtgtaggtcc ttgcctgcac caggctgaac aataatgggg ctaagggcta
      661 ttttcgtatt catggttcct ccaatactgg tttgaccaga tataatttaa gaaacccgga
      721 ttggtctctg tgtccacaat gtagcaaaga ccctttccct tttggtcgcg taaagtgcca
      781 caaaaagcgc gacaaaattt gtcacttgcc ttgtatggaa aaacattcgt ttggctactg
      841 gctcaggctg cgacgcaaag cacttgattt aactcaggat gcgttggctg atcgcgtggg
      901 atgttccgtt gggatgattc gcaagatgga atccgaggaa cgccgtccat cggcacagat
      961 cgctgagcgg ttggcacaaa tattcaacat tccaccgggt gaacatctgg atttcttacg
     1021 tttcgcgcgc ggggacgtga aatctacacc ggttgaaacg gaggaaggtc tgccctggcg
     1081 cgcttcgggt aaatcgccct cttcgaatat ccccgccaca accacatcct tgatagcccg
     1141 tgaaaaagag attgctgaag ttcacgcata cctggcgcag gatgatattc gcctcgtcac
     1201 cctgctcggt ccgccgggca tcggcaagac gaggctcagc atcgagtcgg cgcacagggc
     1261 tcagccggat ttctccgacg gtgcgttttt cgtcgcgctc gctccgctcg acaacgccgg
     1321 cttgatcgcc cagaccattg cccagtcgct tggctttgtg ggtgcaacga acctgcacgt
     1381 cgaagaacaa ctcaaacagg gcatagggaa gaggcacata ctgatcgtgc tggacaattg
     1441 cgagcacctg atcgaagacg ttgcgaggct cgcttatgat ctcctttcag cctgcccccg
     1501 tctatcgatc cttgccacca gccgcgaagt tttgcgcgtc cccggagaat ggctgtatgc
     1561 cgtccctgcg tttgaactgc ccaccgaacg tgctgctctc gatgttgaag gcgcctcgac
     1621 atatcccgcg ttgacgttat tttccgaacg cgctcgggcg gtgcgttcag atttcgcgct
     1681 gagccctgaa aacatccagt ccatcgcagc catttgtgcg cagctcgatg gcttgcctct
     1741 ggcgatcgaa ctgatcgcgg cgcgcatccg attaatgtca ccgcagtcgt tattggaacg
     1801 catgagcggc catttcgttt tgactgcaga cgggatgcgt atcgcttccg ggcgccagaa
     1861 gacactgcga agtgcaattg attggagtta caacctgctt ccacccgaag agcaaaaact
     1921 atttgcttac ctctctgtgt tttctggcgg cttcaccctg gcggctgccg aagcgatttt
     1981 ttcgcgcagt tttaccgaga agtccgtgcc tgaactactt gccttgcttc ttgataagag
     2041 cctggtacgg cgagtatcca gtgaatcgcg tgaagatcgt tatgagatgt tggtgaccgt
     2101 tgaggagtat gctcacgagc gtttacagca ctggggcgag gaagccgaag tccgtaattg
     2161 gcatctggat tattttctta gtcttgccga aaaggcagat aatgaactac gcggaccgaa
     2221 tcaacccgaa tggctgaatc gccttaatgt aatgcacgat aacgtgcgca ccgcattgga
     2281 ttgggggatc gaaacgggtc aaacagaaac tgcactacaa ctggcaagca gactatggtg
     2341 gttctggtta atgcgctcag aattcaatga agggcggcaa tggctgggac gcgtcctggc
     2401 gatgcccgat gtaacatcat tcccttacct gcacgccgcg attctgacac aactagcgaa
     2461 tcatacctgg ctccagagtg aatctgatga agccaggccg attattgagc aggcgctccc
     2521 ggttgcacgc tcactggggg atccacaaac gcttgcatat acactgatgg ttttcgctct
     2581 ggttttgacg tcggaagagg attttgccgc cgcccaatcc acatttgaaa aaagtatgac
     2641 gctctttcgc gaaggtggcg acaaatgggg atatgcgatg gctgtgatgt gcctggcttc
     2701 cgtagcccat agaaacgatg atcaggttaa ggcactcgcg ctgaacgagc aggcactggc
     2761 aggtttccgc gaactcggcg aaacctattt tcaaagtgtc tgcctctggg cgagcggtca
     2821 tcttcgcgca aagctgggcg attggaaggc cggattggaa gagttacgag aatcgctgac
     2881 cctatcacga agattggaca gtaaattgga agtcgcctcg gttttattac gtctcgcaga
     2941 agtcgaagga catctggaag acccggctcg agcagtgcgg ctgtactggg cggcaagaaa
     3001 cgtgtatgac tctattggag tccggtatac gacggataat cccaatttgg aaaaacggct
     3061 agtgtcttgc cgcaccgcgc taggcgaagc cgcgtatgcc gaagcgatgg aacaaggccg
     3121 ccttatgacg agggaacagg cgattgcgta cgctctcgaa gatcaggaat gaaatagtgg
     3181 ccggacgtat ccgaccacta ccctgtgcat tcgtttctat tctacctact cacagttctt
     3241 gtcttatggg aggataatag ccccgctcat cgtgccggag gtcacacctg tggtgatgtt
     3301 tacctcacga ttcagggtga agcttccacc ggcattcgca aaccggcctg ttccgcctgt
     3361 gatcgtaaat tcttcaacaa cgttgaagac atcagtggtt ccggttgggg ttgcctgtcc
     3421 ggacatattt gcataaagaa tgtccccatt cgcagcaaca agttgagcgg atcccacacc
     3481 cgccagggtc tcaaggttga cttcgacctc ataactaatg gcatattgcc caaggtgcgt
     3541 ggcgtttcca gagccatttg cagtcacagc catgatcggc ggggtgacag cataggtttc
     3601 aacagactgc aatgtgcctt tgaaaggcag ctctttcttc cctgctttcg caggtgcagc
     3661 gagcgtagtg cctgccagag caacgacgag caatggggca agggtcgtag atacgattaa
     3721 ctttttcatg gttcctccaa ttctggtttg accagatata atttgcagag atcgaattga
     3781 tctcttgatt gataatgtag caaatccatg atttgcattg gtcactctgg gcgttacaag
     3841 aatcgtgaca gcttgagcga caatacaccg gaatcgaact gacttattta tgcaagcgca
     3901 ttcctttggc aactggttga aaatcaggcg caaagcccgt gatttgacac aggcagaatt
     3961 ggcaaaccag gtgggctgtt cagcagccgc catccgcaaa ctcgaagcgg aggaacgacg
     4021 tccttctgct cagattgttg aacgtcttgc ggaaattttt gaaattccac agaacgaaaa
     4081 agcgaacttc ctgcgctttg cacgcggtga aatgcgatct gcgcctgcgg aaacaacaga
     4141 agattcaccc tggcaggttt ctggcggatt gacgcgttcg aatctaccga taaccgtcac
     4201 ctcgctgatt gggcgcgaaa aggaaattgc cgaggttcgt aactatcttt cgaaggatga
     4261 tatgcgcctc gtcactctga tcggtccgcc gggcataggc aagacgcgcc tcggcattga
     4321 atcggcaggc acactcctcc ccaattttcc cgaaggtgtc ttcttcgttg cgctggctcc
     4381 gctggaggat ccagctatga ttgcgcaagc cgttgcacaa tcgcttggct acgtggggac
     4441 aagaaacata cccgttaccg agcaactcag agagggtatt ggcaggaaac aaatgcttct
     4501 tatgttggat aactgtgaac atttgattga agatgtggct gcactcgctt cttccctcct
     4561 ttcggcctgt tcaggtttga agatcctt
//
LOCUS       DASTJP010000451         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_215237, whole genome shotgun sequence.
ACCESSION   DASTJP010000451 DASTJP010000000
VERSION     DASTJP010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215237"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VFQ23_13055"
     CDS             complement(<1..1457)
                     /locus_tag="VFQ23_13055"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9907572.1"
                     /translation="MFTAYKLLNTGILILFMLVGPFNAGSTQQLVKNSRVLTNNETLI
                     QTSIDSSKLVFEPVISGLSQPVFITNADDGSGRLFVIERIGRIRIGENNMLLPTPFLD
                     MESIVRSTSSEQGLLALAFHPQFESNGYFYTVHTNSSGSIVLSRFTVSPSNSNQASFG
                     SRVELLAIPHPTYTNHNGGTLAFGPDGYLYWSTGDGGGGGDPFNNAQSRNSLLGKILR
                     LDVNSASPYAIPPTNPFFNNPDPAVREEIWSYGLRNPWRFSFDRLTHDIYIGDVGQGN
                     IEEIDFQAASSSGGENYGWDVMEGSNCYEPSSGCNQSGKVLPIAQYSHSLGCSVTGGY
                     VYRGSKYPSLYGHYFYGDYCTGRLFSIYKDPVLGWQPAVELLDTTFNISTFGEDEQGE
                     IYLANYSTGVIYNIGYEDLLTISGNVGMNGVRLSYDDGGSKTAASTGDGSYSFPVSYN
                     WSGTVTPTHPCFTFNPPSVTYSNVTTNQTRQNYTAT"
     gene            1757..>2110
                     /locus_tag="VFQ23_13060"
     CDS             1757..>2110
                     /locus_tag="VFQ23_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9907573.1"
                     /translation="MAPKQYDTIIIGAGHNGLVAAAYLAKQGKKVLVLERRSIIGGSV
                     VTESFGEGFVADSVQTGGTLRHEIIRDLKLSLPTVTERPEFISIQHDGNQLTLNAESI
                     RRFSEKDAARWPEFLR"
BASE COUNT          590 a          513 c          465 g          542 t
ORIGIN      
        1 gtcgctgtgt agttttgtcg cgtttggttg gttgtcacat tgctgtacgt cacactcgga
       61 gggttgaacg tgaagcaggg atgcgtgggt gtcaccgtcc cgctccagtt gtatgacacc
      121 gggaaggaat agctgccatc cccagtcgat gccgcagtct tcgatccccc gtcgtcatag
      181 ctcaacctca cgccgttcat ccctacattc ccgctgattg ttaggagatc ttcataacca
      241 atgttatata ttactccggt cgaatagtta gccagataga tttctccctg ctcatcttca
      301 ccaaacgtag agatattgaa ggtagtatcc agcagttcaa cggctggctg ccagccgagc
      361 acaggatctt tataaatgct aaagagcctg ccagtacagt aatcgccata aaaataatga
      421 ccgtaaagag atggatactt tgatccgcga tacacatacc ctccggtcac cgaacacccc
      481 aggctgtgac tatattgtgc tattggcaaa acttttccac tttgattgca tccactggat
      541 ggttcataac aattactacc ctccataaca tcccagccat aattttcccc acccgaacta
      601 ctggctgcct gaaaatcgat ctcctcgata ttgccttgtc caacatcgcc aatataaata
      661 tcatgagtga gccggtcgaa ggaaaaccgc caggggttac gtaaaccata ggaccaaatc
      721 tcctcacgca cagcaggatc cgggttattg aaaaatgggt ttgtcggtgg gatggcatac
      781 ggcgaggcgg aattgacatc caggcgcaat attttgccaa gcaaagaatt tcggctttgc
      841 gcattgttaa aaggatcgcc acctccgcct ccatcaccgg ttgaccaata caaatatcca
      901 tccgggccga atgccagggt gccaccattg tgattagtat aagtcgggtg cggaatcgcg
      961 aggagttcaa ctctgctgcc aaagcttgcc tgattcgaat tcgatggaga gacggtaaac
     1021 cgcgataaca caatcgagcc ggaggaattt gtatgaaccg tataaaaata tccgttagat
     1081 tcaaattgag gatgaaacgc taaagcgagt aagccttgct ctgagctggt cgaacggact
     1141 atcgattcca tatcaaggaa cggtgttggt aagagcatat tattttcgcc tattcggatt
     1201 ctcccaattc gttcgataac aaataggcgg ccagaaccat catccgcatt tgtaataaaa
     1261 acaggttggc taagtccact gatcacaggc tcaaaaacca atttcgacga gtcaatgctg
     1321 gtttgtatca aagtttcgtt attagttaat acacgtgaat tttttactag ttgttgggtt
     1381 gagccagcat tgaatggtcc caccagcatg aaaagaataa ggatccctgt attaagtaat
     1441 ttataggcag taaacatata gactcctgag gaacggacag tttctctggt tggatattgg
     1501 atttttcttt tgggatggac aggaaaatta tagtgacaac aacccaattt aagctaccca
     1561 ccaaatagcc cactgcttga cccacacttt cgtaaatacc tcgtaagtta acaactgttt
     1621 cgaagaacaa agcaatgcat acacgaaacc cacatactat tcaggcgatt ccctctccat
     1681 ggcaatcaga caattacgag ttgattcaac aggatgtata atcttttcat tccagctacg
     1741 gaagttgaga attttcatgg cacctaaaca atacgacacg atcatcatcg gcgcgggaca
     1801 caatggactg gtcgctgctg cctatctcgc caaacaaggt aaaaaggttc tggttcttga
     1861 gcgccgctcc atcatcggcg gctcggtggt gaccgaatca tttggtgaag gatttgtagc
     1921 cgactctgtg caaacaggcg gaactctgcg tcacgagatt atcagggatc tgaaactttc
     1981 acttccaact gttacagaga gacctgaatt catttctatc caacacgatg gaaaccagct
     2041 taccctcaat gccgaatcca tcaggcgctt ctctgaaaag gacgcggcac gctggcctga
     2101 atttctccgt
//
LOCUS       DASTJP010000452         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_111963, whole genome shotgun sequence.
ACCESSION   DASTJP010000452 DASTJP010000000
VERSION     DASTJP010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111963"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="VFQ23_13065"
     CDS             complement(<1..1386)
                     /locus_tag="VFQ23_13065"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET9907574.1"
                     /translation="MSVLKIRTEHIIKDYPGTRALDDVSVCFDSGRVHALVGKNGSGK
                     STLVKIFAGAIKPSSGEFYLNDERLHFNSTSDAYHKGIVTVYQEMSLVPGLSVAENIF
                     LGRLPKKNQAIDWRKTYQMASLLLQQMKANIDPHEQVSRLSMWQMQVVEITKALSFNP
                     KVIMLDEPTSALAQNEVQNLFTAIRALKDQGVIVIYITHKLQELPQIADTVSVLRDGK
                     LVGTVQMNEVQQKDIINMMFGEVKIQNRPTDIKVRDEFIMEVKNFSRHGWDRDVNFKL
                     KRGEVLGIAGMLGSGRTELLRGIFGADPVDSGEVTVEGKTYKSANPIKMVKAGIGLTP
                     ENRKNQGLILIHSIKDNLCYASMNITADGWLENKRRRNALAERQVKELEIKVNDIDAP
                     CESLSGGNQQKVVVGNWLNTSPKIMLYDEPSRGIDVSAKQQIFEIMWDEARKGVSTIF
                     VSTELEELLEVC"
     gene            complement(1574..2575)
                     /locus_tag="VFQ23_13070"
     CDS             complement(1574..2575)
                     /locus_tag="VFQ23_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9907575.1"
                     /translation="MYSKRIFMVMSLLALFALLMTACGTAATPAEGNTELTIAGVVFQ
                     DDQFMKSMVQGYEDAGAKYGIKILTANTNNDQAKEAELIQTYIAQSVSGIAIAPLSQD
                     ASIPNLKEATEKGIQVAITNLNLTGDPSWLAGGYTSDDATNGKIVGTNAAEFIKATIT
                     GPVNVAQVDFDHQIPEQSKARWSGFYAGLDEAGVEYAKVAAVSANMQDDALAKVTDML
                     TAHPEINVIWASNDGGTIGAAMAVQQAGLAGKVFVFGYDGGDQQTSMILSGDNILVGV
                     VAQDPYAQGYNAVKSLALTLLDQANADAGKITVVPGTYLSVSDLDAVKTWRTDNGLK"
BASE COUNT          611 a          735 c          641 g          754 t
ORIGIN      
        1 gcagacttcc aggagttctt ccagctcggt cgagacgaaa atggttgaaa cacccttgcg
       61 cgcctcatcc cacatgatct cgaagatctg ttgttttgca ctcacgtcga tcccgcgcga
      121 gggttcatcg tagagcatga tcttggggct ggtattcagc cagttcccca ccaccacttt
      181 ctgttgattg ccacctgaca atgattcgca gggcgcatca atatcattta cttttatttc
      241 caattccttc acttgtcgtt ctgccagggc attccgtcgg cgtttattct cgagccatcc
      301 atcggctgtg atattcatgc tggcgtaaca caggttatct ttgatggaat gaatcaggat
      361 caagccttga ttttttctgt tctcgggcgt aagcccgatt ccggctttca ccattttgat
      421 gggattcgcc gacttgtatg tcttcccttc taccgtcact tctccgctat ccaccggatc
      481 cgctccaaaa attccccgca ataactcggt ccggcccgaa ccgagcatcc cggcaattcc
      541 caatacttct ccgcgcttca gtttaaagtt gacatcccgg tcccaaccgt gccgactaaa
      601 gttttttacc tccatgatga attcgtcccg gacctttatg tcggtgggcc ggttctgaat
      661 tttgacttct ccgaacatca tattgataat atccttctgc tgcacctcat tcatttgcac
      721 agtcccaacc aattttccgt cccgcagcac agacactgta tctgcaatct gtggcagttc
      781 ctgcagtttg tgcgtgatgt aaatgacgat gactccctga tctttcagcg cacggatcgc
      841 agtaaaaaga ttttggactt cgttttgggc cagggctgaa gttggctcat ccagcatgat
      901 taccttggga ttgaaactca aggcttttgt aatctcaacg acctgcatct gccacatgct
      961 caaacgggac acttgttcat gcgggtcaat attggctttc atttgttgaa gcaaaagact
     1021 cgccatctga tatgtcttgc gccaatcgat ggcttgattc ttcttcggca accgacccag
     1081 gaagatgttt tctgcaacag aaagacccgg cacaaggctc atctcctgat atacagtgac
     1141 gattccctta tggtaggcat cggaggtgga attgaaatgg agcctctcat cgtttagata
     1201 gaactctcca gaagagggtt tgatcgcccc cgcgaatatt tttactaatg tagatttccc
     1261 ggatccattc ttcccaacca gggcatgtac ccgaccactg tcaaaacaga cactgacgtc
     1321 gtcaagcgca cgggtgccgg gatagtcttt gatgatatgt tccgttcgta ttttgagaac
     1381 gctcatcttt ttacctcaag actttgctat accgcctgtt acataaatta ctaaaccttt
     1441 ggataggaat tacaggtaag tagagtaatt acagtttagt catttatgcg gcacgttgta
     1501 gagaggaaca gggattcctc ctgagaagtc tccctgttcc attgtttcaa tcaatcatct
     1561 tatggtgctg aaactatttc aaaccattat ctgttcgcca cgttttcaca gcatccagat
     1621 ctgagacgct gagatacgtg ccggggacaa cggtgatctt gccagcgtcc gcgtttgcct
     1681 ggtccagaag cgtcagagcc agggatttca ccgcgttata gccttgcgca tacggatcct
     1741 gagccaccac accgaccagg atgttatccc cgctgaggat catactggtc tgctggtcgc
     1801 cgccatcata accaaagacg aacaccttgc cagccagccc cgcctgctgc actgccatgg
     1861 ctgcaccaat ggtgccgccg tcattggaag cccagatcac attgatctcg gggtgagccg
     1921 tcaacatgtc agtcactttc gcaagagcat catcctgcat gttggcactg actgccgcaa
     1981 ccttcgcata ctccacaccg gcttcgtcca gaccagcgta gaaaccactc cagcgtgcct
     2041 tggactgctc agggatctga tggtcgaagt ccacttgcgc cacattgaca ggtccagtga
     2101 tagtcgcttt gatgaactcc gcggcgttgg ttcccacgat cttgccgttg gtcgcatcat
     2161 cggaggtgta gccaccggcc agccaggagg ggtcgcctgt cagattcagg ttggtgatgg
     2221 caacttgaat ccccttctcc gttgcctcct tcaggttggg gatggaagca tcctggctca
     2281 gaggagcgat ggcaatgccg cttacgctct gggcaatgta ggtctggatc agttcggcct
     2341 ctttggcctg gtcgttgttg gtgttggcgg tcaggatctt gatgccatac ttggcaccag
     2401 cgtcctcata gccctgcacc atgctcttca tgaactggtc atcctgaaat acgacacctg
     2461 cgatagtgag ttccgtgttc ccttcggcag gggttgcggc tgtgccacag gccgtcatca
     2521 gcagagcgaa tagcgccaag aggctcataa ccatgaaaat acgtttgctg tacatgttct
     2581 tctccttgaa ttagtacggg aatagatggg aatgtttaat tgagaatgaa agaggtattt
     2641 tgtggtcttt tgactccttt tctaccaggg tgagtagaag atggaataag tcgaacagca
     2701 tttcacgcca tttgtttaca gagcatcaaa atcccattac c
//
LOCUS       DASTJP010000453         5270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1455773, whole genome shotgun sequence.
ACCESSION   DASTJP010000453 DASTJP010000000
VERSION     DASTJP010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455773"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFQ23_13075"
     CDS             <1..541
                     /locus_tag="VFQ23_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9907576.1"
                     /translation="QRGQTVLINGASGSVGTYAVQLALYFGAEVTGVCSTANVELVKS
                     LGAHKVIDYTKEDFTRNGGTYDIIFDAVGKSSFSRCKNSLTQKGIYVTTVPMPDFLLG
                     VLLTSKSKGKKAAFVAPGLRSSSDKAKDLNFLKELIEAGKLKSVIDRRYPLEKIVEAH
                     RYVEGGHKKGNVILTLRSI"
     gene            complement(730..2505)
                     /locus_tag="VFQ23_13080"
     CDS             complement(730..2505)
                     /locus_tag="VFQ23_13080"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9907577.1"
                     /translation="MMNHTADTTSAPYCFISYCRQEVTFADSFSRELEKRGIRTWVDF
                     RNLVPGRPWQDQLDDGVHNAKTILLVVSKASMASLPVKDEWTKSLATGKRIILILFEP
                     CKLDPGLTGLAWVDFTKPFDKAMSKLIGLLARSTQPAPSTLLQKWIHLPSSVMVFASL
                     SLLLVLLCAISIPVAVYSGVQLWVETVVPTSSLTYRLYSTIRFLSLFFIWLPTILNFA
                     WLPIQLFRRTHNAQKIRNTLNALFYSGLLLITLPVILYFQAIIQDLSTSAFFNDFLPM
                     IAVNLVTIVVCLYLNRLLVSNEMYRWSGPNGVLIQSRAPDLTGHTDNGAHMRVAVEYA
                     PQDRLYAQELKASIRKAGHFYTDHVHSADVILCLLSVYKTGSDFDPETKRVIPTLMQV
                     CQLDQHLSRVQWIDLRHGKVSMDAVAHLLDEPAELLRILGVLPVRTTILPNAVKWLSI
                     LLSFFLTAGLVWTATEAAAVVAAFRFIKNTGMAPESLQLDIDMGASILPVVPFLGLYF
                     LRRYIVNRKLRYFPFMSYWWTVGFAILIALLAMSTSGFIQLGWPLLLIPLLMLHKDIR
                     LWLPVSAKKRVREGQRSEVQPAASQ"
     gene            complement(2804..4435)
                     /locus_tag="VFQ23_13085"
     CDS             complement(2804..4435)
                     /locus_tag="VFQ23_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET9907578.1"
                     /translation="MPQYDVIIVGAGAGGGVAAGVLAEAGKRVLLLERGPHLAFRDNR
                     RDHLRNQRFSRYGHNAGPEIEGNPRVAMDAQGQALVVRPHQNGYHNNAAVVGGGTRVY
                     GAQAWRFLPQDFRMASLYGVPSGSSLADWPITYDELEPYYERAEWEIGVAGDSPASAR
                     HWPRRKGYPMPPLPVNQQGQLLRAGASKLGWETLPVPALINSVPYNARPACIQCGYCV
                     GFTCPVEAKNGTHNTLILRALATGRCELVTEAMVVSIDTDSSGHVSGVTYFDQAGQRH
                     SVRSEVVICAAGAIETARLLLNSTSSQHPHGLGNQHDQVGRHLQGHYYPAAIGLMSES
                     VYDGLGPGISTATVQFNHGNSEVLGGAMLADEFIELPVIFWARQLPPDLPRWGMANKR
                     FMRENYTRVMRVTGPVQEIPNPQARVTLDPTVHDRWGIPVARLSGTTHPETVRTAAFM
                     FESAREWLEASGAIRVWGQPPGLYLSAGQHQAGTCRMGDDPLTSVVDQWGRVHGHDNL
                     FVTDGSVHVTNGGFNPVLTILALAFRASEHIAQTW"
     gene            complement(4438..4842)
                     /locus_tag="VFQ23_13090"
     CDS             complement(4438..4842)
                     /locus_tag="VFQ23_13090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HET9907579.1"
                     /translation="MLTALHWNTLRALMNRIIPPDDFPDAWDAGVGDYLARQFELDLR
                     SQLEMYHLGLEALEAESFASAGTGFTELDPSAQDAILSRLEAGEAMSSWTVDPAEFFR
                     TVTEHVVEGYYSDPGNGGNRDSIAWRMIGFEK"
BASE COUNT         1346 a         1370 c         1341 g         1213 t
ORIGIN      
        1 ccagcgcggg caaactgtcc tcatcaacgg cgcttcgggg tcggtcggta cgtatgctgt
       61 tcagcttgcc ctatactttg gggccgaagt aaccggcgtg tgcagtaccg ccaatgtaga
      121 gttggtgaaa tcactgggcg cccacaaggt cattgattac acaaaagaag actttaccag
      181 gaacggtggg acctatgaca ttatcttcga tgcggttggc aagagttcat tttcgcgctg
      241 taaaaactcg ctcacgcaaa agggcatcta cgttaccaca gtgcccatgc ccgattttct
      301 gcttggcgtg ttattgactt caaaaagcaa ggggaagaag gcagcctttg tggccccggg
      361 tttacgttcg tccagtgaca aggcgaagga cctaaacttc cttaaagagt tgatcgaggc
      421 gggaaagcta aaatcagtca ttgaccggcg ctaccctctg gaaaaaatcg tcgaggctca
      481 tcggtacgtt gaaggagggc acaaaaaggg aaatgtgatc ttaacgttgc ggagtatcta
      541 acatacaaat gattttgtca ttctccaaag gacaccgctt tggacggtgt gaacgaccgt
      601 agggagcgaa gggtgacatg ttccctctct ccttgagttt agatacatac tctctaactt
      661 tggaaaataa taaaccgtga accatctgcc accgtccctc ggcgtggatc tccccaagcg
      721 atgcgctctt tactgagagg ctgcaggctg gacctcagat cgctgaccct cgcggacccg
      781 ttttttggca ctaactggaa gccagagtcg aatgtctttg tgtagcatca acaacggaat
      841 aagcaatagg ggccatccca attggataaa tccggaggtg gacatggcta gtagggcaat
      901 caatatagca aaccccactg tccaccagta ggacatgaaa ggaaaatacc ttagcttgcg
      961 gttaacgatg taacgcctga gaaaatagag tccaagaaac gggacaacgg gcaatatcga
     1021 tgcacccata tctatatcta gttgaagact ctcaggagcc attccagtat tcttaatgaa
     1081 acgaaaggcg gcgacaactg ctgcggcctc tgttgcggtc cacaccaatc ctgcagtcag
     1141 gaaaaacgaa agtaaaattg acagccattt gacggcgttg gggagaatgg tcgtacgaac
     1201 cggtagaaca ccgagaatac gcaataactc ggcaggttca tctaacaaat gggcaaccgc
     1261 gtccatggat acctttccat gacgcaggtc gatccattgc acgcgcgaca aatgttgatc
     1321 taactggcag acctgcataa gggttggtat cacacgtttg gtttctggat caaagtccga
     1381 acctgtcttg tatacagaaa gcaggcagag aatgacatcc gcgctatgaa cgtgatccgt
     1441 gtagaaatgc cctgcttttc taatggatgc tttcaactct tgagcatata gcctgtcttg
     1501 tggagcatat tcaactgcca cgcgcatatg agctccattg tcggtatgtc ctgtcaggtc
     1561 aggcgctcta ctttgaatga gaacgccgtt gggtccggac cagcgataca tctcattcga
     1621 aacaagcagg cgattgagat agaggcatac cacaatggtg actagattta ccgcaatcat
     1681 tggaagaaaa tcattgaaaa atgctgacgt ggataaatct tgaatgattg cctggaaata
     1741 taggattaca gggagtgtta ttaataacaa accggaataa aacaatgcat taagcgtatt
     1801 cctgatcttc tgagcattgt gagtgcgccg gaacaattgt atgggtagcc aggcgaaatt
     1861 taggatggtg ggcaaccaaa tgaaaaacaa agataggaat cggatagtgc tataaagcct
     1921 ataagtaagg gatgaggttg gcaccacggt ctccacccac agttgcactc cagaatatac
     1981 ggctacgggt atgctaatag cgcacagaag gactaaaagg agacttaggg aagcgaatac
     2041 catcacagaa gacggtaggt gtatccattt ttgcagcagc gttgacggag ctggttgggt
     2101 tgatcgggct agaagtccga tcagtttgct catggccttg tcaaagggtt tggtgaaatc
     2161 tacccatgcc aaacctgtca aacctgggtc taatttgcat ggctcgaaga ggatgaggat
     2221 gatccgtttg cctgttgcga gggacttcgt ccattcatct ttcactggca gggaagccat
     2281 cgaagctttt gaaactacta atagaatagt ttttgcatta tgtaccccat cgtccagttg
     2341 atcctgccag ggtcgcccag ggacaagatt gcgaaagtcc acccaggtac ggatgccgcg
     2401 tttctccaac tccctcgaaa agctatctgc gaacgtgact tcctgccggc aataactgat
     2461 gaagcaatat ggggcagacg tagtatcggc tgtgtggttc atcattcctc atggcttttc
     2521 tccaacctct ggcttttaag gacccgtcct tgccgctgag cgcagcgctc aatctatcca
     2581 tcttgtccct gtcgctgcgt gcattaaaaa ataacccatc tcccctcatt tcgtcgcgag
     2641 cagatcctct tcctcaatcg ccaaccaatt tcccgcttcg tcacgcgatg taaactgtaa
     2701 tgtttgcgct acgcgaaaat cccgaagggt ccagagcaat ctgccgatca taagcattgt
     2761 cacaactagt ataagggttc acatttcgaa aacttagtgc gcattaccaa gtctgcgcga
     2821 tgtgctccga cgcgcggaaa gccagtgcca gaatggttag caccgggttg aaaccaccat
     2881 tggtgacatg gaccgaccca tccgtcacaa agagattgtc gtgcccatgt acccgtcccc
     2941 attgatccac taccgaagta agcgggtcgt cacccatgcg gcaggtccca gcctgatgct
     3001 gaccagcgct caaatacaga ccaggtggct ggccccacac gcggatggcg ccagaagcct
     3061 ccagccattc gcgtgcgctc tcaaacatga aggccgctgt gcgcacggtc tcgggatgag
     3121 ttgtgccaga cagtcgcgcc actggaattc cccaacgatc atgcacggtg gggtcaagtg
     3181 tcacgcgcgc ctgcgggttg gggatctcct gtaccgggcc agtcacgcgc atcacgcggg
     3241 tatagttctc gcgcatgaag cgcttgttcg ccatacccca gcgtggcaga tctggtggca
     3301 attggcgtgc ccaaaagata accggcaatt caatgaactc atctgcaagc attgcgccgc
     3361 cgagtacctc actgttgcca tgattgaatt gaaccgttgc ggtagagatg cccggtccga
     3421 ggccatcgta gaccgattca gacatcaggc cgatcgcggc tgggtagtaa tggccctgca
     3481 agtgacgtcc cacctgatca tgctgattac cgagtccgtg tggatgctgt gatgacgtcg
     3541 agttcaataa gagacgggcg gtttcaatcg caccggcggc gcaaatcacc acctcagatc
     3601 gtacgctatg ccgttgtcct gcctgatcaa agtacgtaac accgctgaca tggccggaag
     3661 aatccgtatc gatgcttacg accatggcct cggttaccag ttcacagcgc ccagtggcta
     3721 aagcgcgaag gatgagggtg ttatgcgtgc cgttcttcgc ctccaccggg caggtaaacc
     3781 caacgcaata tccgcactga atgcatgccg ggcgagcatt gtatggcacg ctgttgatca
     3841 gcgctgggac aggcagagtc tcccaaccaa gtttggacgc gccagcgcgc aataactggc
     3901 cttgctgatt cactggtaat ggcggcatcg ggtaaccttt gcgacgtggc cagtggcggg
     3961 cgctcgccgg gctatcgcct gccacaccga tctcccactc cgcgcgctca tagtagggtt
     4021 ccagctcatc ataagtaatc ggccaatctg ccagggaact gcctgatgga acgccataga
     4081 ggctggccat gcggaaatcc tggggcagaa atcgccaggc ctgcgcgccg tatacccgcg
     4141 tgccaccgcc taccacagcg gcattgttgt ggtagccatt ctgatgaggc cgcacgacaa
     4201 gcgcctgtcc ctgggcatcc attgccacgc gtgggttgcc ttcgatctct ggtccggcgt
     4261 tgtgtccata gcgggaaaaa cgttgattcc ggagatgatc gcgtcggttg tccctaaatg
     4321 ccaaatgagg tcctcgctcc aacagcagca cacgcttgcc tgcctccgcc agcacgcccg
     4381 ctgcgacgcc accgcccgcc ccagcaccta caatgatgac atcgtattgt ggcattgtta
     4441 tttctcaaaa ccaatcatgc gccaggcaat ggagtcgcga ttaccaccgt tgcccgggtc
     4501 actgtaatag ccttctacca catgctcagt cacagtgcgg aagaattcgg caggatccac
     4561 cgtccatgac gacatggctt cgccagcctc aaggcggctg aggatcgcat cctgcgctga
     4621 aggatccagt tcagtgaaac ctgtacctgc ggaggcaaaa gactctgctt ccaatgcctc
     4681 cagcccaaga tgatacatct ctaactgcga gcgcaagtcg agttcaaact ggcgtgcaag
     4741 atagtcgcct actcctgcgt cccacgcatc cgggaaatca tccggtggga taatacgatt
     4801 catcaaggca cgcaatgtat tccagtgaag tgctgttagc ataagattaa atgcaaaatc
     4861 aattgtagac aaaacgcgct caacaaaggt ctgtaatcga cttttcttgc tatggcagtg
     4921 tccaaaagga taggataaat ttttctcagc cacagagcac acggagttca tggagaaaac
     4981 ctcaaaattc tccgtggtct ctgcggttaa gcccacaaaa ttagacaccc tgcttgctat
     5041 tagaaaaagt ggccttgtta gccttcttgt attataggca tttctatgtg tgggacaatg
     5101 cctttcatta ggttaacttt agttgcacaa agtaaaaagg gacagtcaac tcatgattga
     5161 ctgtcccttt ttacttctct catcgcacca aaaacctctt cacatcatca ggcaggcctt
     5221 ggctaatcag atattggaat atgtattatc aattagagac gggttgatcc
//
LOCUS       DASTJP010000454         9550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_434468, whole genome shotgun sequence.
ACCESSION   DASTJP010000454 DASTJP010000000
VERSION     DASTJP010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434468"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VFQ23_13095"
     CDS             <1..742
                     /locus_tag="VFQ23_13095"
                     /inference="COORDINATES: protein motif:HMM:NF040422.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HET9907580.1"
                     /translation="LLPSADSVITKHDIEVEAVHVEVGVGSPIPVHAIAEMNLPGTCA
                     QLGEIRMHREGNTFFVRLIADVSERADCIVDSIPFRLEIPLNIVNLPEGPYEVNINGT
                     TASFDPRTTPVSDSPLITLERTACFGTCPIYTLAIFEDGRVEYIGQDFVTITGEQTGS
                     ISTEQVKELLAAFESADYFNLQDQYTAPITDLPTTITTLSFEGKTKTISNYGGCMPDL
                     LIPAPQALCDLEIMIDEITNSAQWVGNS"
     gene            946..1416
                     /locus_tag="VFQ23_13100"
     CDS             946..1416
                     /locus_tag="VFQ23_13100"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9907581.1"
                     /translation="MKSQTARATRIINAPAETIYNIIADFRNQHGQILPTQYFQALEV
                     EEGGFGAGTITRFQMTLLGQTKSFRTLITEPEPGRVLIETDIQSGTPTTFTVSPLEDG
                     DGSRVEIATELRSENMAENLIGKFMLQKVYRAELELLARQAEKQGLLVNSASAN"
     gene            1450..1824
                     /locus_tag="VFQ23_13105"
     CDS             1450..1824
                     /locus_tag="VFQ23_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907582.1"
                     /translation="MNKMNASHTIQALGAGWQSLEKLELPVNSDLETILEIRLMEILA
                     SLKLQSDFQNRILRSTLEASRRVVASINQENHNRHIHIHIFIPENTEVNGKTWGFFRI
                     QRLEVQAELSKHTIELYLYKED"
     gene            complement(1864..4437)
                     /locus_tag="VFQ23_13110"
     CDS             complement(1864..4437)
                     /locus_tag="VFQ23_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9907583.1"
                     /translation="MTTEISFGAWVRRRRKSMDLTQQQLAQRLGCSISLIFKIEADER
                     RPSRQIADLLVKHLEIPEEQRELFLRVARQERTIDHLEEASSPTSPQAGAASTNIQPG
                     LPNPLTAIIGREHELRAIVQQLESPACRLLTLTGPGGIGKTHLSLEVAHQMKNMYSDG
                     VCFVSLVGTGTAEFIVPAIAEALGVSFSGAFELKNQLFNYLKDRHMLIVLDNLEHLLN
                     GVELLDELLERAPRVKLLTTSREQLNLHAEWIFEVQGFPLPVGLQFESADSNSAVAFF
                     VQRAKQTHSDFNLTPDNLPYVERICQLVEGLPLGLELAAAWMRVLSVEEVAHEIERSM
                     DFLTTSTRDLPHRHRSMRAVFDYSWNLLSSEEQNVLRRLSVFKGGFQRAAAEQVAGAN
                     LRLLSSLVSKSLIHRHTAQAGRYHQHELVRQFAASYLMKDVPKDMAVRSRHAEYYLAF
                     WYDSENQLQSARQREILSELMADINNFRAAWDQAMAQNQWELLGQALRVLLLVYDLRG
                     WHTEGVERLRPIVQALESDPDPHRKNGNVLGTALSFLGWFHFRRGQLLEARQFLERGL
                     AILRTRSEKQGLAEALTLLAPVFTSLGEGKQALANVSEALEVARAGGDKWHEAYALMM
                     QGGILSGWGEYDQAYASSEEALKQFRLIGDIRLIIVTLNTLGFVSLRSARYAEAREFL
                     KESLALITPADDPWTAATAYGNLGIVELAEKNYAEARDLFQKSIPIFTNLGMNGDVAF
                     YLTYLGETCAAMGATKEAEGHWLNAIHIAHETQAVPTILANLMRLAQLYAERGDIARA
                     YEWTTQILSHPSAWEETKSRATKFSAELGSQLSSQQIQDAQADAKSKALEAIVHELGM
                     S"
     gene            complement(4503..7076)
                     /locus_tag="VFQ23_13115"
     CDS             complement(4503..7076)
                     /locus_tag="VFQ23_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9907584.1"
                     /translation="MDDSFGTWIKRRRKSLDMTQQELAKRVGCSNSLIFKIESDERRP
                     SRQIAELLVRHLEIPNEQRELFLKVARQERSVEHLPGKLASPASQPVSIPVSAKLPHP
                     LTSLIGREHELRAIMQQLQDPDCRLLTLTGPGGVGKTRIALEVAHQDHEYEPCFVSLA
                     GTSASEYIIPAVADAIGFAFSGATELKKQLFQYLKDQRVLIVLDNLEHLLDGIEVLDE
                     LLVVAPHAKLLTTSREPLGLQAEWVFEVQGLPVPANVESDKLETNSAVALFVQRAKQA
                     DAKFMPSAENLSAVIRVCQLVEGLPLGLELAATWVRVMSVQEIASEIERNMDFLTTTA
                     RDMPQRHRSMRAVFDYSWELLTEHEKRSLQQLAVFRGGFTREAAERVTGVTLQQLSAL
                     VDKSLLRHTNTQSGRYDFHELIRQYAEAKLEGLPDEYAQTRERHSEYYADLLYRWKDP
                     LQGSAQQSILPQTSLEIDNLRLAWDWIVTNRQITKLDKSLESMFVLHDVRNWLHQGAT
                     LFAQAVAAVKSLDRQDEKGNENDRLLGSFMVAQGHMCWHLGYMQDARELLQQGLQLLR
                     PFGSSHMLSESLLYLSILEQSQGNYQTARGLADECVALNEKQGRPSGSGYALSNLGMI
                     CLLQGEYESAYAYIEKGTALMRSIGYARGIAINQTRLAAASLRLGRVAEARELLEDSL
                     HTTRSLRDRWGIGNALNYMGLVESARGNFGQAEMFLREGATLFQEDGDQIQFVSILTD
                     LGFLLIERRNESESRQVFGQALEKALQSQAIPVALSSLVGFGKLYAREDATEFALELV
                     LFAENHPSSSQQTKDRAGMLHRTLEGRLTSDQMRVVQIRAWNTTFETLRQKILNRQFR
                     N"
     gene            7233..7868
                     /locus_tag="VFQ23_13120"
     CDS             7233..7868
                     /locus_tag="VFQ23_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9907585.1"
                     /translation="MNSQQQLQNLDEETLLSSASNGDLEAFNQVVLNYQNLAFSHAFS
                     MLGDSDLAEDATQDSFIKAFQALSSFRGGSFRGWLLKIVTNSALDLLRRARRHPNQPL
                     FPQDENGEEIESASWLVDPAASVQEVVEEKEFSRDIYKILDGLPEVFRSVITLIDINE
                     LDYSEAAEALMVPIGTVKSRLARGRMQMQAKLRNCLGNGTVLGVRDLSLCV"
     gene            complement(7942..8148)
                     /locus_tag="VFQ23_13125"
     CDS             complement(7942..8148)
                     /locus_tag="VFQ23_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907586.1"
                     /translation="METNNASNQFEPWDVEVAKRIACAVTSYQAGVPYESLWEQTKNQ
                     EESIGTFWLSIAKRIREEMPKKSK"
     gene            complement(8135..8356)
                     /locus_tag="VFQ23_13130"
     CDS             complement(8135..8356)
                     /locus_tag="VFQ23_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907587.1"
                     /translation="MSKTTARWIYWGMFFYILATISVAVLVIAFHIHPTYSAPLMCTY
                     TLSIFMLMIVHPFKRWVTRERKKLFYGDQ"
     gene            complement(8802..9188)
                     /locus_tag="VFQ23_13135"
     CDS             complement(8802..9188)
                     /locus_tag="VFQ23_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907588.1"
                     /translation="MLIPAEIILDTILEERADIPINAIHMYSGLKADLYPVREGDDLR
                     QSAFQRREQVDYGPPIGKVYIHSPEDLILYKLMYFGLSQQSKHSRDIAAILKSKKSEL
                     DLDSIEGWAARLGLSSLWKEMLDNIS"
BASE COUNT         2362 a         2461 c         2342 g         2385 t
ORIGIN      
        1 tttgcttccg tcagccgatt cggtcattac caaacacgat atcgaggtgg aagcagtcca
       61 tgtggaagtc ggtgtggggt cacccatccc tgtacatgcg atcgcagaga tgaacctgcc
      121 gggcacctgt gcgcagttgg gtgagatccg catgcaccgt gaagggaata cctttttcgt
      181 ccggctgatc gccgatgttt cagaacgcgc ggattgcata gtagatagca ttccattccg
      241 tctggagatt ccgttgaata ttgtcaatct tcccgaaggt ccatatgaag tcaacatcaa
      301 tggaacgacc gccagttttg acccccgcac cactcctgtt tctgacagtc ccctgatcac
      361 actcgaacgc actgcctgct tcggtacttg tccaatttac acgctcgcta tttttgaaga
      421 cggtcgcgtg gagtatatag gccaggattt tgtgactata acgggcgaac agactggcag
      481 catttcaact gaacaggtga aggaattgct cgcagcgttt gaaagtgcag actacttcaa
      541 tttgcaggac cagtacaccg ctccgatcac cgacttgccc acaaccatca caacgcttag
      601 ctttgagggc aaaaccaaaa cgatcagcaa ctatggcggc tgtatgccag acctgctcat
      661 cccggcaccc caggctctat gcgacctgga aatcatgatc gatgagataa cgaattcagc
      721 acaatgggtg ggcaattctt aagggcaatt tgagtgtgca atacgaaaga ctccccagta
      781 gggagtcttt ttttattccg cgtcgaacag cgatttcctg attgaagtta atggatgtat
      841 gactttttgt ctgtgttttt gtacagcttt tccagaccta tgacgaaact tttttttgta
      901 taactgcgtt atgactacga tcaatattca cagaggagat agatcatgaa atcacaaacc
      961 gccagagcga caagaatcat caacgccccc gccgaaacca tctacaacat catcgcagac
     1021 tttcgtaacc agcatgggca gatcctgcca acgcaatatt tccaggcgct ggaagtggag
     1081 gaaggagggt ttggcgcagg gacgatcaca cgctttcaaa tgactctgct tgggcagact
     1141 aaatcctttc gtaccctcat tacggagccc gagccggggc gagtgcttat cgaaaccgat
     1201 attcagtccg gtaccccaac cacctttacc gtttcacctc tggaagacgg agatggcagc
     1261 cgggtcgaga tcgccaccga gttaagaagt gaaaacatgg cagagaatct catcggaaaa
     1321 tttatgctgc aaaaagtgta ccgcgccgag ctggaactgc tcgcaaggca ggcagaaaaa
     1381 caaggtctgc tcgtaaattc agcttctgca aattagcgca tcattgctca acatgaattc
     1441 ggaacgaacg tgaacaagat gaacgcatct cataccatcc aggctctggg agctggctgg
     1501 cagtcacttg aaaaactcga attgcctgtt aattcagatc tggaaacgat tcttgaaatc
     1561 cgcctgatgg aaatactggc ttcgctcaaa ttgcagtcag attttcagaa taggatattg
     1621 agatctactc tggaagccag ccggcgcgtc gtggcgtcga tcaatcaaga gaatcataat
     1681 cggcacattc acatacatat attcattcca gagaacaccg aagtcaatgg caaaacctgg
     1741 ggtttcttcc gaattcaaag actggaggtc caggcagagt tgtctaagca cacgatcgag
     1801 ctatatcttt ataaagaaga ttgacacgaa gttagtcctt tgtgcatgca aagaatgcat
     1861 caactaactc atccccagtt cgtgcacgat ggcttccaac gccttagact ttgcatctgc
     1921 ttgtgcgtct tggatttgtt gggaggaaag ttgcgatcct aattcagccg aaaattttgt
     1981 ggcacggctt ttcgtctctt cccaggcgga aggatggctt aagatttgag tcgtccattc
     2041 ataggcgcgg gcaatatcac cccgttccgc gtagagctgt gctaaccgca tgagattggc
     2101 aaggatcgta ggcacggcct gagtttcatg agcgatatgt atcgcattca accagtggcc
     2161 ttcggcttct ttggtcgcgc ccatcgcagc gcaggtttcg ccaaggtacg ttaaataaaa
     2221 cgcgacatcg ccgttcattc ctagattcgt aaaaatggga atactctttt ggaacagatc
     2281 gcgcgcctcc gcatagttct tttcagccag ttccacaata cccaaatttc cgtacgctgt
     2341 tgcagcagtc cagggatcgt cggccggggt gatgagggcc aggctctcct tcaggaactc
     2401 gcgcgcttca gcataccgag ctgatctcaa tgagacaaag cccagggtgt ttaaggtcac
     2461 gatgatcaga cgtatatctc cgatcaagcg aaattgtttc agcgcctcct ctgagcttgc
     2521 ataagcctgg tcgtactccc cccagcctga gaggataccg ccctgcatca tgagtgcata
     2581 tgcctcatgc cacttgtccc cgccggcacg tgccacctca agtgcctcac ttacattcgc
     2641 taaggcctgc ttgccttccc caaggctagt aaaaacgggc gccaacaaag tgagcgcttc
     2701 agccagccct tgtttctcac tccgggtacg gaggatcgcc agtccgcgct ccaggaattg
     2761 gcgcgcctcc agcaactgcc cacgccgaaa atggaaccaa cccagaaaag ataaggccgt
     2821 ccccaggaca ttgccatttt ttctgtgtgg atcgggatcg gactcaagag cttgcacgat
     2881 cgggcgcagg cgttcaacgc cctccgtatg ccagccgcgc agatcataaa ccagaagaag
     2941 cacgcgcaag gcctgcccca ggagctccca ttgattctga gccatagctt gatcccaggc
     3001 cgcgcggaag ttgttgatat ctgccatgag ttcagacaat atttcgcgct gcctggcact
     3061 ttgtaattgg ttttcactgt cataccagaa cgcaaggtaa tattccgcat gacggctacg
     3121 cactgccata tcctttggca catccttcat gagataagag gcagcaaatt gccgaaccag
     3181 ttcgtgctga tgataacgcc cggcttgtgc cgtatggcga tggatgagtg atttgctcac
     3241 cagagaagat aacaatcgca gattggcccc ggcaacctgt tcagcggctg cgcgttggaa
     3301 tccgccttta aaaaccgaaa gcctcctgag cacattttgt tcctcgctgg aaagcaggtt
     3361 ccacgagtaa tcaaatacag cccgcatgga ccggtgacga tggggcaagt cgcgcgtgga
     3421 cgttgtgaga aaatccatgc tacgctcgat ctcgtgcgca acttcctcga ccgaaaggac
     3481 gcgcatccag gctgcggcca gttcaagccc gagcggtaag ccttccacga gctggcagat
     3541 gcgctccacg taaggaaggt tatcaggcgt gaggttgaaa tcagaatggg tttgttttgc
     3601 gcgttgcacg aagaacgcca cggcgctgtt cgagtccgcg ctttcaaact ggagtccgac
     3661 tggcaaagga aaaccctgca cctcgaagat ccattctgcg tgtagattca attgttcacg
     3721 agaggttgtc agcaatttga cgcgcggcgc tcgctccaga agctcatcca gtaattcaac
     3781 cccgttcaac aaatgttcca ggttgtcaag cacgatcagc atatgcctgt ctttaagata
     3841 gttgaacaac tgatttttca attcaaacgc gccagagaac gaaactccca gtgcctctgc
     3901 aatggccggc acgataaact cggctgtgcc tgtcccaacc agggagacaa agcagacgcc
     3961 atctgagtac atgttcttca tttggtgcgc cacttccagc gaaagatgcg ttttaccgat
     4021 gccaccagga ccggtcagag tcaatagacg acaggctgga gactctaact gctggacaat
     4081 tgctcgcagt tcgtgctcac gcccgataat cgcggttagt ggattcggca ggccaggctg
     4141 gatgtttgta gatgcggcac cagcttgcgg tgaggtaggc gaagaagcct cttcaaggtg
     4201 atcgattgtc cgttcctgcc tcgcgactct taagaacaat tcgcgctgct cctcgggaat
     4261 ctccagatgt tttacaagca aatcggcgat ctggcgcgac ggacgccgtt catccgcttc
     4321 gatcttgaag atgagcgaaa tagagcaacc caggcgttgt gccagctgct gttgggttag
     4381 atccatcgat ttgcgcctgc gcctcaccca ggcaccaaaa ctaatttcgg tggtcatggt
     4441 ttttctaaag caactatatt tgattttcca aaatttggga actcaatcgg ggtgagtaaa
     4501 tttcaatttc taaactggcg gttgagaatt ttttgccgaa gcgtttcgaa cgttgtattc
     4561 caggccctga tttgtacgac tctcatttgg tctgaagtta atcgcccttc gagtgttcga
     4621 tgcagcattc ccgctctgtc ctttgtctgt tggctgctgg acggatggtt ttcagcgaac
     4681 aaaaccagct ccagggcgaa ctcggttgca tcttccctgg catacaactt tccaaatccc
     4741 accaggctgg ataaagcaac cggaatcgcc tggctttgaa gggccttttc aagggcttgc
     4801 ccaaatacct gacgggactc agattcgttc ctgcgctcga tcagtagaaa acccaaatct
     4861 gtgaggatgg atacaaactg gatctggtca ccgtcctcct gaaaaagtgt cgcgccctca
     4921 cgaagaaaca tttcagcctg tccgaaattc ccgcgcgcag actctaccag ccccatataa
     4981 ttgagtgcgt tcccaatccc ccagcggtca cgcaggcttc gtgtcgtatg gagactatct
     5041 tcaagcagtt cacgtgcttc ggcgaccctg ccaaggcgca aagaggctgc cgctagacga
     5101 gtttggttta tggcaatgcc gcgtgcatac ccgatggaac gcatcagcgc tgtccccttt
     5161 tcgatgtatg cataggcaga ctcgtactct ccctgtaata ggcagatcat gccgaggttg
     5221 ctcaacgcat agcctgagcc agatggtcta ccctgctttt cattcaacgc aacacattca
     5281 tctgcgagcc ctctggcagt ttgataattg ccctgtgact gttcaagaat cgaaagatag
     5341 aggagggact ccgagagcat gtgagagcta ccaaacggtc tgagaagttg aagaccctgt
     5401 tgaagcaatt cccgagcatc ttgcatataa cctaaatgcc agcacatatg cccctgggca
     5461 accataaaag aacccagcaa acggtcgttt tcatttcctt tttcatcctg gcgatccagt
     5521 gacttgaccg cagcgacggc ttgtgcgaaa agagtcgcgc cctgatgaag ccagtttcgc
     5581 acatcatgca ggacgaacat actttccaga gatttatcca gcttggtgat ctgacgattt
     5641 gtcacaatcc agtcccaagc caggcgtaaa ttgtcgattt caagactggt ctgcggcaag
     5701 attgactgct gcgcggaacc ttgcagggga tctttccaac gatacagcaa atctgcataa
     5761 tattcgctat gccgttccct cgtctgggca tattcgtcag ggagtccttc gagttttgcc
     5821 tccgcatact gacgaatcag ttcgtgaaag tcatatcgcc cggactgcgt gtttgtatga
     5881 cgcagcaacg atttatctac cagcgcagaa agttgttgaa gtgtcacccc ggtgacgcgc
     5941 tcagccgcct cacgcgtgaa acctcctcga aataccgcca gctgctgtag ggaacgtttt
     6001 tcatgctcgg tcagaagttc ccaggagtag tcgaagactg cccgcatcga acggtggcgt
     6061 tggggcatgt cgcgcgctgt ggtcgtcagg aaatccatgt tccgttcgat ctcgctggca
     6121 atttcctgca cagacatcac tcgcacccag gtagccgcga gttcgagccc caatggtaag
     6181 ccttccacga gctggcagac gcggatgacc gccgataagt tttccgccga aggcatgaac
     6241 tttgcatctg cctgttttgc ccgctggaca aataatgcta ccgcgctgtt cgtctccagc
     6301 ttgtcggatt caacgtttgc aggcacgggg agtccctgca cttcgaatac ccattcggct
     6361 tgcagtccga gtggttcacg cgaagtcgtc aacaatttgg catgcggcgc gaccacgagc
     6421 aattcatcaa gaacctcgat gccatcaagt agatgctcga ggttatcgag cacgatcaag
     6481 acgcgttggt ctttcaagta ctgaaaaagt tgttttttca attccgtggc accggaaaac
     6541 gcgaacccaa ttgcatccgc cacagcagga atgatgtatt ccgaggcgct ggttccggcc
     6601 aacgagacaa agcaaggttc gtattcgtgg tcttgatgtg ccacttcaag tgcaatccgc
     6661 gtcttgccaa ccccgccggg tccagtcagt gtaagcagac gacagtcggg atcctgcagt
     6721 tgttgcatga tggcgcgcaa ctcatgctca cggccaatca gggaagtgag tggatgaggc
     6781 aacttcgccg agacgggaat tgaaacaggc tgggatgcag gcgaagccag ctttccgggg
     6841 aggtgttcaa ccgacctctc ctgacgggcc actttcagga ataactcgcg ttgttcgttt
     6901 ggaatctcca ggtgcctgac gagcaattcc gcgatctgcc gcgaagggcg gcgttcgtct
     6961 gattcgatct tgaagatcaa tgaattggaa catcccaccc gttttgccag ttcctgctgg
     7021 gtcatatcca gtgactttcg ccgccgcttg atccaggtac cgaaggaatc atccatatat
     7081 catcacttga agttccaatt atgggctttt gcgtaattgt actatttttt gtccagtgat
     7141 ttgtggaggt tattcacacc aacgattccg aaaaaagaat aaatttggga tgcgttcaat
     7201 cgcacatcgg cagcattcac gaggagaccg tcatgaactc acaacaacaa ctccaaaatt
     7261 tggatgaaga gactttgctt tccagcgcat caaacggaga tctggaagcc ttcaaccagg
     7321 tcgtactgaa ctatcagaat ctggcattta gccacgcctt ttccatgctt ggtgactctg
     7381 atttagctga agatgccaca caggatagct ttatcaaggc atttcaagcg ttgtcgtctt
     7441 ttcgtggggg ctcgtttcgc ggctggctgc tcaaaattgt taccaattcg gcgctggatc
     7501 tcctgcggcg cgcccgcagg cacccgaatc agcctctatt tccccaggat gagaatggag
     7561 aggaaattga atccgcaagc tggcttgttg accctgctgc gtcggtgcag gaggttgtgg
     7621 aggagaaaga attctccaga gatatttaca aaatcctgga tggtctgcct gaggtattcc
     7681 gcagcgtcat tacattgatc gatatcaatg agcttgatta ttcagaggct gcagaagctt
     7741 taatggttcc aatcgggacg gtcaagagtc ggctggcgcg tggccgtatg caaatgcagg
     7801 cgaagcttcg aaattgtctg ggcaacggaa ccgttctcgg cgtcagagat ttgagtctgt
     7861 gtgtgtaaag gattaacaaa aaaggctccc attccgggag tctttttatt gtagcttgaa
     7921 gcagaatcat tgcttgccta tttattttga tttctttggc atctcttccc gtattcgttt
     7981 tgcaatggaa agccaaaaag tgccaatgga ttcttcctga tttttagttt gttcccataa
     8041 gctctcatac ggaacgcctg cctgataaga ggtcacggca caggcaatgc gttttgcgac
     8101 ttccacgtcc cacggctcga attgattgct tgcgttattg gtctccataa aacaatttct
     8161 tcctttctcg ggtcacccag cgtttgaacg ggtgaacgat catcaacata aaaatactga
     8221 gcgtatatgt acacatcaac ggcgcactat acgtggggtg aatgtgaaag gcaataacca
     8281 atactgcaac tgaaattgta gccagaatat aaaaaaacat tccccaataa atccagcgag
     8341 ctgtggtctt cgacattcgg aaattgttca taatccagtt ctctttgttg atccactttg
     8401 ttgaaaacgt acaaccgatt gaacaataac acattctccc cccgggggac attgaacgtt
     8461 agtaggatta ttcaggtaca gttcttgcgt agtttgaacc gggcacctca tgtaccgatt
     8521 actgacggta tccgactccg cctcaagtcg gggtgccgag atttgcgaag tcccagaagg
     8581 gggaactcgg gacctcacga acccgaacac tttcaaaaaa ttcgaactgt agtccacatg
     8641 gtcaggtggt tttttatagc cgtgaggtca acctttttgg gtaaccatgt ggactaggct
     8701 gagattaaaa caatactctg ttgggtcgaa aattgaattc cgagtacagc aatattacca
     8761 ctttttgaaa tttgacgttc catcgtacat attactggat ctcaagaaat gttgtcaagc
     8821 atctccttcc atagcgaact caatcccaag cgggctgccc agccttcgat agaatccagg
     8881 tcaagttcac ttttctttga tttgagaata gccgcgatat ctcgcgagtg tttcgattgc
     8941 tggctgagtc caaagtacat gagcttatag agaatgagat cctctggtga atggatgtac
     9001 acctttccaa tgggcggacc ataatccact tgttcacgtc gttgaaatgc gctttgccga
     9061 aggtcatccc cctctcgtac tggatacaaa tccgctttga gaccactgta catgtggatg
     9121 gcattgatcg ggatatctgc acgctcttcc aagattgtat ctaagatgat ctcagccgga
     9181 atgagcatgt ctctcttctt caactccttc gataacttgt tgatagattt aattgggatt
     9241 ttgactacga gatcaagatc ttgagtggcg cgcggctctc cccatgccta ttctgcaatc
     9301 gcaccgccaa ttagatattc gaccccgaca gccttcagcg cttcgatcac taacttgaga
     9361 aaaccagcaa tatccaatgg tttcttcgtc attggttata cgctctatcc agtgcgatcc
     9421 gatttgcctc agcgagtgac aatcgtgggt tttcctgatg aatgcggtaa gccacgacct
     9481 tgcgagcgat atcactgatg gaacaaccct ggtgaaagcg ttctgcaaag gataatttgc
     9541 ggtaaatgtc
//
LOCUS       DASTJP010000455         8120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_962740, whole genome shotgun sequence.
ACCESSION   DASTJP010000455 DASTJP010000000
VERSION     DASTJP010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962740"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFQ23_13140"
     CDS             complement(<1..777)
                     /locus_tag="VFQ23_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9907589.1"
                     /translation="MAKLDFLTQEIAGLKEQGLYNNIRTIGSAQGARLIVDGKDVLNF
                     CSNNYLGLANHPKIVEAAKDATKKYGVGPAAVRSIAGTTDLHVQLEERLAKFKGAEDV
                     ITFQSGFTANLGAISALVGKEDVIFSDRLNHASIIDGSRLSGAKIVAYEHNDAGALED
                     VIKEHASKYRRSLIITDGVFSMDGDIAPLPDLVEVAKKYDILIMVDDAHGEGVLGKGG
                     RGIVDHFDLHGQVDIEIGTMSKAFGVVGGIVAGDKVIIEWL"
     gene            complement(1007..1852)
                     /locus_tag="VFQ23_13145"
     CDS             complement(1007..1852)
                     /locus_tag="VFQ23_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9907590.1"
                     /translation="MASLKGKVAIVTGASRGVGKGIALGLGEEGATVYVTGRSTEEKT
                     DVEKLGGTVFSTAEAVTAMGGKGIALHCDHREDSQVEDAFKRIAKESKRIDLLVNNAW
                     GGYEKMREGRAFTYFKPFWEQPFWRWDAMFDAGVRAAYTASALAARLMTKKKSGLIVN
                     ISFWSAQVYMNNTQYGVSKAAVDKMTEYMAHELKKYKVAVLSLYPGLVRTESVMRSAK
                     HFDMSNSESPQFIGRVVAALARDPHIMKKSGQVLVAAQEALEYGIQDIDGKQPRPVTL
                     EDFKK"
     gene            2007..5156
                     /locus_tag="VFQ23_13150"
     CDS             2007..5156
                     /locus_tag="VFQ23_13150"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9907591.1"
                     /translation="MSAKPLHEQIANLKIAIEAQETMRSILGDAVVETTLGVLRDQLE
                     ELEKQLTQQDMANQARRKLVTVLFADVSGFTAFSENMDAEDVANLVNHLWVRADRIIF
                     EYGGHIDKHIGDAVMAIWGADAAREDDAERAIRAALKMQEIFKENLGQLPVGMKVGIN
                     SGQVLLSAIGTQGEFTAIGDTVNTASRLVTSANPGEILISHDTYQLVRGVFDIQIQSP
                     IMVKGKREPLQTYVVQRVRPRAFRIGRRGVEGVTTHMVGRGAEFQVIQSALEQCVKDR
                     HVTVVIVKGEAGLGKSRLIYEFEAWLDSHPTDFYLFKGRADEESMSLPYSLVRDVFSE
                     RFQIRDSDSQAEAREKLVKGMSAMIGSAQEERAAFIGEMLGFDFSESAYVRGIRNDAR
                     QIRDRAFSNIAQFFEELTHDSPVILLLDDIHWADNGSLELIEYLAKLETDLPIFIMCA
                     TRPRLDESHPNWGSSFPLCEVITIKSLDLSASRELVNEILQNVSDLPEELVETVAKNA
                     EGNPFYLEELVKVLIEDGVIVKGEEIWQIIPQRLTQLRVPATLTGVLQARLDRLPSRE
                     NEVLERAAVIGRTFWDAAVEAMHGSETAMVLTGGADVHSALSALKNKELVFSRQPSTF
                     ATAQEFIFKHAILREVTYERVLKAKRKLYHRLAADWLIQQSGERVDEYLGLIAQHYEL
                     AGDTVSAVEFLERAAEQSMRLSAYREALSASERALGILAASEDTNPAMRARLLLTIGS
                     AHLWLTDHATATARFEECIALAHTIHDQSLESKALARLGRIGLEQGKFEQSERHLQES
                     LAIAQELNDIDVIAYTLAHLGYISHYQSRYENAQRYGVESYEFAKKTGDAIAQAFSLN
                     MLAMISVNNNQFEQARQYHLQAIEISKNAGDRYGLARAYSNLSELIRIQRKYAEAKPY
                     TLEGIKLGRELGNRYMIPIMLVNLVYSQVGLGEIREAYASVREALQFNVEGDSTSWIL
                     FSLVGYANILAAEGKREQALEVLGLCMHHPDTNSDTHRDIQLVLEDLSKIRSDDIEVG
                     LERGRSLDIKDVVITALSG"
     gene            complement(5153..6751)
                     /locus_tag="VFQ23_13155"
     CDS             complement(5153..6751)
                     /locus_tag="VFQ23_13155"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HET9907592.1"
                     /translation="MPDFFDSLLQKDLGFLRIIAELWGLELDSKNADAAREKLTIALL
                     DPNLFAELIDSLSPEAEAAIDALVNAGGRIPWPTFTRRFGEIREAGAGKRDRTKPHLN
                     PTSAAEVLYYRGLLGRSFFDTEKGPQEFAYLPDDLLSLLSREEREEKKEKLGSDPAPL
                     GRPASPGEKGQEIPATDSILDDATTLLAALRTGKSDGSSDRRLYELLKSAKLIENHVL
                     HPEAVKSFLEASREESLKMLQDAWVESIQFNELRLMPGIICEGEWRNQPQETREFLLN
                     LLDAIPEGKWWSLNAFVRDVKQKYADFQRPAGDYDSWFIKRVSDEQYLRGFESWDEVD
                     GALVRFFITGILHWLGQVDVAIADGAKAPTSFRITSIVHQKEERGKITVASSGKITIP
                     RDAPRVVRYQISRFCEWEEPKSDVYRYHLTTNSLNKAKEQGLKVEHLLALLARHSDAG
                     VPPVIVKALKRWEANGTEARVQTQVILKVSRPEILEEMRKSKAAKYLGEVLSPTAVIV
                     KSGAIQKVIDALTELGLLSEVITD"
     gene            complement(6796..7713)
                     /locus_tag="VFQ23_13160"
     CDS             complement(6796..7713)
                     /locus_tag="VFQ23_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9907593.1"
                     /translation="MPRKVKIILNPMADMGNAWRVARDLRSITVEHGSVDWSGTVYPG
                     HAIELAKQASIEGYDMVIAMGGDGTVHEVVNGIMQAPENKRPILGVVPVGSGNDFAHG
                     IGASKNSTQALTRALTGEVSTVDLGLMTDENGRKEYFDNTLGMGFGAIVTIRSHKLPV
                     LRGFLMYLAAVIQTIIIDHNPMRMQIETDEQKWEQSVIYLVLCNGPREGGGFLIAPDA
                     KIDDGIIHYAMITDVSRPMMFRIVPEVMKGTHGRFKQVRMGTCRQLTLTSNRPLYIHA
                     DGEIFSGPGSDIHKVRVEILPNALKVIRG"
     gene            complement(7837..>8120)
                     /locus_tag="VFQ23_13165"
     CDS             complement(7837..>8120)
                     /locus_tag="VFQ23_13165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9907594.1"
                     /translation="LILTQSDMSSAQIVAEKIVEGMGKLKYKLPDGRKLKLGISGGIA
                     IFPVNARSGPDLLRAADAALYQAKKHRRGAFEVARGITGPLATHRIGAE"
BASE COUNT         1945 a         2152 c         2018 g         2005 t
ORIGIN      
        1 cagccactcg atgatgacct tatcccccgc gacgatgccc ccaaccacac caaaggcttt
       61 ggacatggtg ccaatttcga tgtcgacttg gccgtgcaaa tcaaagtgat cgacaatgcc
      121 acgtccgcct ttgccaagca cgccttcacc atgcgcatcg tccaccatga taaggatgtc
      181 gtatttcttc gcgacctcga ccagatcagg aaggggcgca atgtcaccat ccatgctgaa
      241 gacgccgtcg gtgatgatca gggaacggcg atactttgaa gcatgctctt tgatgacatc
      301 ttccaaagct ccagcatcat tgtgttcata cgcaacaatc ttcgcgcctg agagtcgact
      361 gccgtcgata atcgacgcgt gattcaagcg atcagaaaaa atgacatctt ctttcccgac
      421 cagcgcggaa atggccccaa gattcgccgt aaacccagat tgaaacgtaa ttacatcttc
      481 ggcacctttg aatttggcga ggcgctcctc cagttggacg tgtaaatccg ttgtccccgc
      541 gatcgaacgc acggccgcgg gaccgacacc atattttttg gtcgcatcct tagcggcttc
      601 aacgattttt ggatggtttg ctagcccaag atagttattg gagcaaaagt tgagcacatc
      661 cttaccatcg acgatcagcc gcgcaccctg cgccgaacca atcgtgcgga tgttgttgta
      721 taaaccctgt tctttcaaac cagcgatctc ttgcgtgagg aaatcgagtt tggccataag
      781 gactcctgtg tttgttatac cggtggttga gacgcaagca gtcgaaacta ccctatttca
      841 aggaatgttt tgcttggaga ttacgtgctg gtctcgatat ggactcacct gcactgcacc
      901 aaacaaagtg caggtgcaag tgtcgtccta ctcgaccagc gggttatatt tttcattata
      961 gcaacttaac aaaaaagcga ctgccgcgac tttctggaac agtggactat tttttaaagt
     1021 cttcaagcgt cacaggtcgc ggctgtttgc catcaatatc ttgaatgccg tactccagtg
     1081 cctcctgtgc agccaccaaa acctgaccac tctttttcat gatgtgcgga tccctggcca
     1141 gagcagcaac cactcgacca ataaactgtg gcgactctga attgctcatg tcaaaatgtt
     1201 tcgcgctccg catgacactt tctgtacgaa cgagacccgg atacagcgaa agtacagcca
     1261 ccttatattt tttcagttca tgcgccatat actcggtcat cttatctacg gcagctttgg
     1321 aaactccata ttgtgtatta ttcatataaa cctgcgccga ccagaaagaa atgttaacaa
     1381 tcaagccgct ctttttcttt gtcatcagtc gggctgccaa tgcagaggct gtgtatgccg
     1441 cgcgcacgcc tgcatcgaac atggcgtccc agcgccagaa tggctgttcc caaaagggtt
     1501 tgaaataggt aaaggctctc ccctcacgca tcttttcata tccgccccag gcattgttga
     1561 ctaaaaggtc gattcgttta ctctcttttg caatacgctt gaacgcatcc tctacctgtg
     1621 agtcctcacg atggtcacag tggagcgcga ttcccttgcc gcccatcgcc gtcacagcct
     1681 cagcggtcga gaagacagtg ccgcctaact tttcgacatc ggtcttctcc tcggtcgagc
     1741 ggcccgtcac atagaccgtt gcaccttctt cgcccaatcc caacgcgatt cccttaccca
     1801 cgccgcggct cgctccagtt acaattgcaa ctttcccttt cagagaggcc ataccaactc
     1861 cttgataatt aatgtttggg tcgctgatta aaacaggctt tgaacgttaa agcaagggag
     1921 ccacaaattg caatgaaagt gttcgtgttg agttagttac agaattgaga catgccagga
     1981 aattcacttt ataattttta cagactatga gcgccaaacc tctgcacgag cagattgcca
     2041 accttaaaat tgctattgaa gcccaggaga ccatgcgctc gattctgggg gatgctgtcg
     2101 ttgagacgac tctgggagtc cttcgcgacc aactggaaga actggaaaag cagcttacgc
     2161 aacaggacat ggcaaatcag gctcgccgca agctggtgac cgttttgttt gcggacgtct
     2221 ccggttttac cgcgttctcc gaaaacatgg acgcagaaga tgtggccaac ctcgtcaatc
     2281 acctgtgggt gcgagcggac cgcatcattt tcgaatatgg cgggcacatc gacaaacaca
     2341 tcggcgacgc ggtcatggca atctggggtg cggatgcggc ccgggaggat gacgcagaac
     2401 gcgccatccg cgccgcgcta aagatgcagg agatcttcaa agagaacctg ggccagttgc
     2461 ccgtcggcat gaaggtggga attaacagtg ggcaggtttt attaagcgcc attggcaccc
     2521 agggcgaatt caccgctatc ggcgatacgg tcaacacagc cagtcgtttg gtgacctccg
     2581 ccaacccggg tgaaatcctg atttcccacg atacctatca actcgtacgc ggcgtgttcg
     2641 atatacaaat ccagtcgccg atcatggtca aagggaagcg ggagccgctg caaacctatg
     2701 tggttcagcg cgtgcggccg cgcgccttcc gaatcggacg gcgcggcgtg gaaggggtaa
     2761 caacacacat ggtgggccgt ggagcagaat ttcaggttat tcaatccgca ctggagcagt
     2821 gtgtgaagga tcgccacgtc actgttgtca tcgtcaaagg cgaggccggg ctgggcaaga
     2881 gtcgtctcat ctacgagttc gaagcctggc tcgattcgca cccaaccgat ttttatttgt
     2941 tcaagggccg cgccgatgaa gagtccatga gcctgccata tagcctggtg cgcgatgtat
     3001 ttagtgagcg ctttcagatt cgagatagcg actcgcaggc cgaagcgcgt gaaaagctgg
     3061 tcaagggcat gtcagccatg atcgggtcgg cacaggagga acgagctgcc tttatcggag
     3121 aaatgcttgg gtttgatttt tccgagagcg cctatgtacg cggcatacga aacgatgccc
     3181 gccagatccg tgatcgtgct ttcagtaata tcgctcagtt ttttgaagaa ctcacgcatg
     3241 attcaccggt catccttttg ctggatgata ttcactgggc cgataatggt tcgctggagc
     3301 taattgaata tctggcaaaa cttgaaacag atttaccaat atttattatg tgtgcaacgc
     3361 gcccgaggct ggacgaatcg cacccaaatt ggggcagcag ctttccgctc tgcgaagtga
     3421 ttacgatcaa aagcctggat ctctctgcca gccgtgagtt ggtcaatgaa atcctgcaaa
     3481 atgtctctga cctgcccgag gagttggtgg aaaccgttgc aaaaaatgca gagggcaatc
     3541 cattctatct ggaagagttg gtcaaggtgc tcatcgaaga tggggtgatc gtaaaaggcg
     3601 aagaaatctg gcagatcatc cctcagcgac tgacccaact gcgcgtcccg gccacactaa
     3661 cgggtgtatt acaagcccgt ctcgacaggc tcccttcgcg ggagaatgaa gtgcttgaac
     3721 gcgcggcagt gatcggccgc accttctggg atgcagcggt ggaggcaatg cacgggtcag
     3781 agacggccat ggtgctcacc ggtggtgcgg atgtgcattc cgctctcagc gcgcttaaga
     3841 acaaggagct tgttttctca cgtcagccat cgacttttgc aaccgcccag gaattcattt
     3901 ttaagcatgc gattttgcga gaggttacct atgaacgcgt gcttaaagcc aagcgtaagc
     3961 tctatcaccg tttggctgca gattggctga ttcaacagag cggtgaacgc gtggatgagt
     4021 atcttggatt gatcgcgcag cattatgaat tagcgggcga taccgttagc gcagtggaat
     4081 tccttgagcg cgccgcagaa caatccatgc gactcagtgc ctatcgggag gcattgtcgg
     4141 cgtcggagcg tgccctggga atccttgccg cttcagaaga tacgaacccc gccatgcggg
     4201 cgcggctctt gcttaccatc ggctctgcgc atctctggct gaccgaccac gccacagcca
     4261 ctgcacgttt tgaagagtgc atcgccctgg cacataccat ccatgaccag agtcttgaat
     4321 ccaaagcgct ggcgcggctc ggacgcattg gtttggaaca gggcaaattt gaacaatcag
     4381 agcgacattt acaggaaagc ctggcgattg cacaggaatt gaacgatatc gatgtcattg
     4441 cctacacgct agcacatctt ggatatatta gtcactacca gagccgatac gaaaatgcac
     4501 aacggtatgg cgtggaaagt tatgagttcg ccaagaaaac gggcgatgcc attgcacagg
     4561 ctttctcact gaacatgctg gcgatgatca gtgtaaataa taatcagttt gaacaagcgc
     4621 gccaatacca tttgcaggca attgaaattt ctaaaaatgc cggtgaccgt tatgggcttg
     4681 cacgcgccta tagtaacttg agtgaactca ttcgcattca gagaaaatat gcagaagcca
     4741 aaccctacac cctggaaggc atcaagctcg ggcgcgaact tggcaaccgc tatatgatac
     4801 cgatcatgct ggtcaatctc gtttattcac aggtggggtt gggagaaatc cgcgaggctt
     4861 atgcctcggt acgggaagcg cttcaattca acgtagaagg tgattcaacg tcgtggatct
     4921 tattttcact tgtgggttac gcaaatatcc tggcggctga agggaaacgc gagcaggcgc
     4981 ttgaagtcct gggcttatgc atgcatcatc ccgacaccaa cagcgatacc caccgcgaca
     5041 ttcaacttgt tcttgaagat ttgagcaaaa tacggtcgga tgatatcgaa gtggggctgg
     5101 aacgcggcag atcgctggac atcaaagacg ttgtaattac cgccctgagc ggctagtctg
     5161 taatcacttc actcaataat cccaactcgg tcaaggcgtc aatcactttt tggatggctc
     5221 cgcttttaac gatcacggca gtcggactta atacttctcc caaatatttc gccgctttcg
     5281 attttcgcat ctcctccagg atctcgggcc ggctgacttt tagaataacc tgagtctgga
     5341 ctcgcgcttc ggtcccgtta gcttcccagc gtttgagggc tttgacaatc accggaggaa
     5401 ctcctgcatc ggaatgcctt gccagcaatg caagcagatg ctccactttc agcccttgtt
     5461 ccttcgcttt gttcagcgaa tttgttgtaa ggtgatagcg atagacatcg ctctttggtt
     5521 cttcccactc acaaaatctt gaaatctgat aacggaccac gcgtggtgca tcgcgcggaa
     5581 tcgtgatctt cccgctggat gcgaccgtga tctttccgcg ttcttccttc tggtgaacga
     5641 ttgaggtaat acgaaaggag gtcggcgcct tggctccatc ggcaatcgct acatcaacct
     5701 gccccagcca gtggagaata cctgtaatga agaatcgaac gagcgcgcca tccacttcat
     5761 cccagctttc gaagccgcgt aagtattgtt catcagagac ccgtttgatg aaccaggagt
     5821 cgtagtcacc ggccggtctc tgaaagtctg catatttttg cttgacatcg cgaacaaaag
     5881 cattcaggct ccaccatttg ccttcaggaa ttgcatccaa taaattcaat aggaactcgc
     5941 gcgtttcctg gggctgattc ctccactccc cttcacagat gatgcctggc atgagtctta
     6001 actcattgaa ctgaatactt tcgacccacg catcctgaag catcttcaac gattcttcgc
     6061 gtgacgcctc cagaaaggat ttgacggcct ccgggtggag cacatggttt tcaatcagct
     6121 ttgctgactt gaggagctca taaaggcgcc gatcggatga cccatcagat ttacctgtac
     6181 gcaacgcagc cagcaacgtt gttgcatcat caagaataga atcagttgcc ggaatttcct
     6241 ggcctttttc gcccggagag gcaggtctac ccaacggcgc cgggtcagat cctagtttct
     6301 ctttcttttc ctcgcgctct tcgcgactca gaagagaaag taagtcatcc ggcaggtacg
     6361 caaattcctg tggtcctttt tctgtgtcaa aaaacgatcg acccagcaag ccacgataat
     6421 acaatacctc tgctgcagaa gtcgggttga gatgcggttt tgttcggtcg cgtttgcccg
     6481 cgccggcttc ccgtatctcg ccgaatctgc gtgtgaacgt gggccacggg attcgtcctc
     6541 cggcgttgac cagtgcatca atggcagcct ccgcttcggg cgaaagagag tcgattaact
     6601 cggcaaacag atttggatcc aggagagcaa tggtcagttt ttcccgcgct gcatctgcat
     6661 ttttggagtc caattccagt ccccatagtt ctgcgatgat gcggagaaat ccaaggtcct
     6721 tttgtaggag tgagtcaaag aaatcaggca ttctagcaag acagggaaag acggctgcct
     6781 atccctgcgg caattttatc cgcgtatgac tttgagcgcg ttcggtaaaa tctcgacgcg
     6841 taccttgtga atatcactac cggggcccga aaagatttct ccatcggcat ggatgtacaa
     6901 gggccggttt gatgtaagcg tgagctgtct acatgttccc attcgaactt gcttaaatcg
     6961 accatgtgtg cctttcatca cctccggcac aattcgaaac atcatcgggc ggctgacgtc
     7021 tgtgatcatg gcgtaatgaa taatgccatc atcaatcttt gcatccggtg cgatgaggaa
     7081 gccgccgcct tcacgcggac cattgcaaag tactaaatag atcacacttt gttcccactt
     7141 ctgctcatct gtttcgatct gcattctcat cgggttatgg tcaatgataa tcgtttgaat
     7201 cactgcggca aggtacatca ggaagccacg taacactggc aacttgtgtg agcgaatcgt
     7261 gacgatcgcg ccaaaaccca tgccgagcgt attatcaaaa tattccttac gcccgttttc
     7321 atcagtcatc agcccgaggt ctactgtgga tacttccccg gtcaatgcgc gtgtgagcgc
     7381 ctgggttgaa ttcttcgatg cgcctatgcc atgtgcgaag tcattcccgg aaccaaccgg
     7441 gacaacgcct aaaattggtc gtttattttc aggggcttgc ataatgccat tgaccacctc
     7501 atggacagtt ccgtccccac ccatggcgat gaccatgtca tacccttcta tactcgcctg
     7561 tttcgccaat tcaatcgcgt ggcccggata caccgttcca ctccaatcta cactcccatg
     7621 ttcgaccgta atcgatcgga ggtcgcgtgc aacgcgccag gcattgccca tatctgccat
     7681 cgggttcaga atgattttaa ctttgcgtgg catgagattt ctccgtgata ataaaacccc
     7741 aaaggttctg cttgccgatt gttgtgttta cggacgaaac ctttagggtc tatgattttt
     7801 ttctttttat tataaccccg caaaaccgat gagatgctat tcagctccaa tacgatgggt
     7861 tgccaatgga cctgtaatcc cgcgtgcgac ctcaaaggcg ccacgcctat gttttttggc
     7921 ttgataaagg gcagcatccg ccgcacgcaa tagatccgga ccactgcgag cattgacagg
     7981 gaatatggca atcccccctg aaatgcccaa tttgagtttt cttccatcgg gcagcttata
     8041 tttgagctta cccattcctt cgacgatttt ctcggcgacg atttgggcgg acgacatgtc
     8101 tgactgagta aggatcaggg
//
LOCUS       DASTJP010000456         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_773883, whole genome shotgun sequence.
ACCESSION   DASTJP010000456 DASTJP010000000
VERSION     DASTJP010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773883"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFQ23_13170"
     CDS             <1..394
                     /locus_tag="VFQ23_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="putative C-S lyase"
                     /protein_id="HET9907595.1"
                     /translation="EVVRGLRVHVASLGMRAAQAAFSGQCDDWLKELRCYLTGNRDFL
                     IDYVTRNMPDVRITVPRATYLAWLDFTQLDLDKTPHEFFLQKAKVALSDGAIFGENGK
                     GHVRLNFGTSRRILKQGLDRMSRALHSG"
     gene            complement(655..1104)
                     /locus_tag="VFQ23_13175"
     CDS             complement(655..1104)
                     /locus_tag="VFQ23_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907596.1"
                     /translation="MKITKTGTLSGCVVWIIGFGILSACLLPTAMMIGGVTSVSNFAM
                     QTIGPLICPEGTVGRSYSYATTTTDEYGNSQPSTAYELHCVDSNGEVVKEDPVAYAFI
                     WIGSLAAIGLVIAALLAFALAAPAGVLIARLLDRNKKTNIAANIEPE"
     gene            1189..1701
                     /locus_tag="VFQ23_13180"
     CDS             1189..1701
                     /locus_tag="VFQ23_13180"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9907597.1"
                     /translation="MRLIIFSGLPGTGKSTLAEAVGRGLSVPVFAKDWLEATLLRSGL
                     KATDDRALGFAGYELLTILAERQLMLEQSVILDSVAASQTIRSTWHKLSDQYAAQYRV
                     IECICSDEELHRSRLHGRKRNIPGWHELEWSEVERVKRYYFPWEGERLVLDMTQSFTE
                     NFLKAKTYCE"
     gene            1756..>2259
                     /locus_tag="VFQ23_13185"
     CDS             1756..>2259
                     /locus_tag="VFQ23_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HET9907598.1"
                     /translation="MTTIDLTIEKSRRANAIKRAKERNIVIPTYAQMKDPSRIPAKVK
                     DELKNIGLWDIHPRNLFRINWHNEPVASGGTYGGVNYLELPSSLTGVPARIVVLVGKW
                     FPTGAHKVGAAFSCLVPRLVTGQFDPTTQKAVWPSTGNYCRGGAYDSALLACDSIAIL
                     PEGMSKER"
BASE COUNT          575 a          548 c          586 g          550 t
ORIGIN      
        1 agaagttgtc agaggtttac gggtgcacgt agccagcctg ggaatgcggg ctgcacaagc
       61 cgcgttctcc ggtcaatgcg atgactggtt gaaggaattg cgctgctatc taactggtaa
      121 tcgggacttc ttaatcgatt atgtgacaag aaatatgccg gatgtgcgta taactgtgcc
      181 ccgggccaca tatcttgcct ggcttgattt cacgcagctt gatctcgaca aaactcccca
      241 tgaatttttc ttgcaaaaag caaaggtagc tctgagtgac ggcgctatat tcggtgaaaa
      301 cggcaaagga catgtgcgtc tcaacttcgg cacgtcgcgt cgtattctca agcaggggct
      361 ggaccgtatg tctcgcgcac ttcactccgg ttaaaaaagg acggatgggt attaaccatc
      421 cgtcaattgc taaatttgct gttcaggtaa ggagtgggga ttgccacctt cctatcgtta
      481 ggagaacgtc tggttcaaag cggagcttta gccagttcag ggcgtcccag tcctaccttg
      541 ggtaaacatt tcaggtggtg actctatggg tacctccttt ctactatgca tccatcatac
      601 aacgaacaga agccccttac attaaaatta ctttacaagt ttgttataaa ggtatcattc
      661 aggctcaatg tttgcagcaa tgttggtttt tttgttgcga tccaataatc gtgcaatcag
      721 cacacccgcg ggcgcagcca gtgcaaaggc aagcaaggca gcgatgacta gaccaatagc
      781 tgccaggctt cctatccata taaatgcata tgcaacaggg tcttccttca caacttcgcc
      841 attcgaatcc acacaatgga gttcgtaagc agtactgggt tgggaattgc catactcgtc
      901 ggttgtcgtt gtcgcatagg aataggatcg tccaactgtt ccttccggac aaatgagcgg
      961 tccaatggtc tgcatggcaa aattactgac cgaggtaaca ccaccaatca tcattgcagt
     1021 cgggagaaga caggcgctca agatgccaaa accaatgatc cagacaacac atccggataa
     1081 agtccctgtt tttgtgatct tcatgatttc tccttgatga ggcgaaaata acaagatcca
     1141 tctagtttat cctcatgttc tgcaaagtaa aagcggagtg gagccaaaat gagattgatc
     1201 atcttctctg gattgcccgg aacggggaaa agtacactgg cagaagcggt agggcggggt
     1261 ctcagcgttc cagtatttgc gaaggattgg ttggaagcaa cgcttcttcg cagcggtttg
     1321 aaagcgaccg atgatagagc gctcggattt gctggctacg aattgttgac aatattggcc
     1381 gaacgccaac tcatgttgga gcaatctgtg atcctggaca gtgttgcggc gtcacagacc
     1441 attcgcagca cctggcacaa gttgtcagac caatatgcgg cgcagtaccg cgtgatcgag
     1501 tgtatctgct ctgatgaaga gcttcatcgt tcgcggctgc atggacgaaa gcgcaacatc
     1561 ccgggctggc atgagctgga atggtccgaa gtggagcggg tgaagcgata ctattttccc
     1621 tgggaggggg agcgattggt gctggatatg acacaatcct ttactgaaaa ctttttgaaa
     1681 gctaaaacct attgtgagtg aatcgattgt gagatataat acaagttgtc tgacaacttt
     1741 gcttaggaga actccatgac aaccatcgat ctgaccatcg aaaaatctcg ccgggcaaat
     1801 gccatcaagc gagccaaaga acgcaatatc gtcatcccta cctacgcaca aatgaaggat
     1861 ccttccagga tccctgcaaa agtaaaggat gagctgaaga atattggtct ttgggatatt
     1921 catccgcgca acctcttccg catcaactgg cataacgaac ctgtcgcttc aggcgggacc
     1981 tacggcgggg tcaactacct tgagcttccc tcctcactga caggcgttcc ggcgcgcatc
     2041 gttgtgttgg tcggcaaatg gttcccgacc ggcgcgcata aagtgggtgc agcgttcagt
     2101 tgtttggtgc cgcgcctggt cacaggtcag tttgacccga ccacgcaaaa ggcagtctgg
     2161 ccctccacag gcaattattg ccgcggcggc gcgtatgact ccgcactgct ggcatgcgac
     2221 tcgatcgcga tcctgcccga aggcatgtcg aaggaacgg
//
LOCUS       DASTJP010000457         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1065278, whole genome shotgun sequence.
ACCESSION   DASTJP010000457 DASTJP010000000
VERSION     DASTJP010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065278"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFQ23_13190"
     CDS             <1..451
                     /locus_tag="VFQ23_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907599.1"
                     /translation="EATLQNQQIHHIQKGLSSSFVKDVTLSGLVPELKERLEKEGIRT
                     ALDISEEAIARIVVVDAANCEALLQWRSSLHAQFEATQPVKLPDHQLAYIQQKFNRSH
                     ARNDEKERNLKEILQQFEGELQSVQHRLEQFAPITFRGYFLHTLTSR"
     gene            complement(834..>2098)
                     /locus_tag="VFQ23_13195"
     CDS             complement(834..>2098)
                     /locus_tag="VFQ23_13195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9907600.1"
                     /translation="HTENQLRQNQESLRHYAEEIAMTNRELAEARDRALEASQLKSTF
                     LATMSHEIRTPMNAILGMGELLLNTDLNEEQREFASIIDASARNLLNLLNDILDFSKI
                     EAGKIVIRSEAFSLRAAVQETVRLFQPQANEKQLTVELIVDANLPETLVGDAGRIRQV
                     LSNLISNAIKFTEIAGAITISLSGTCVHSNVLMATLSVQDTGSGIPDGLRPYLFEPFT
                     QADGTNTRKHGGSGLGLAISQRLVRLMQGEIGYESMEGNGSTFWFRLPLSIRPEPKMS
                     IVCPPPSHQPPAYRQYAGHKPILVVDDSLINRDLVRLQLKEFGLRSQSATNGQEAVEL
                     LKVDHDSYAMVLMDLYMPEMDGFTAAQLIRQNEAGKSTPIPLVALTASVLSDCRESCS
                     AVGMNDFLRKPVSLADLDRLLAKWLKDV"
BASE COUNT          494 a          559 c          525 g          520 t
ORIGIN      
        1 ggaggcgacg ctgcaaaacc agcaaatcca tcacattcaa aaaggcctgt cttccagttt
       61 tgtcaaagac gtaacccttt ccggactggt cccggagctg aaagaacgcc tggaaaagga
      121 aggcattcgg accgctctgg atatctcaga ggaagccatc gcccgcatcg tggttgtgga
      181 cgcagcgaac tgtgaggctc tgcttcaatg gcggtcatcc ctgcacgcac aattcgaggc
      241 gacccagccc gtcaaattac ctgatcatca actggcatat atccagcaga aattcaaccg
      301 gtcgcatgcc cggaatgacg agaaggaacg gaaccttaag gagatcctgc aacaattcga
      361 aggcgagctg cagtccgttc agcatcggct cgagcagttc gcgccaatca cctttcgggg
      421 ctatttcctc catacactga cgtcccggtg atataccgca aacgacaaga aatctgctca
      481 acgagcgggt ttttgattta accacgtggg agcctgtatt tgtcatgcat cgcggaggtt
      541 cggcattctc cgccgtcgcg atgccaattg tgacccggca ctacacgatg ctttgacaaa
      601 gacaaccccc tcctgcctcc atctgcagtg ggaggggtat gctctggctt tcataaacag
      661 cgcaattgtc tcttgtttca tcccaggttt attccccttc cctttacgaa gcactctgta
      721 gagtacatct gcactttgat ttttggatcc ctactctcct gtaattccag gcctgcagga
      781 ggaagcagcc agaaaatgga tgctatgatg agttggtaaa ccttgggtat cccttacaca
      841 tccttcagcc attttgccag taatctatcc agatcagcca gtgagacagg cttgcggagg
      901 aaatcattca ttcccactgc tgaacaggat tccctgcagt ctgataaaac acttgctgtt
      961 agtgctacta acggaattgg cgtactctta cctgcttcat tttgccggat cagctgtgct
     1021 gctgtgaacc catccatttc cggcatgtag aggtccatca agaccatggc ataggaatcg
     1081 tggtcaacct taagcagctc tactgcctcc tgaccattcg tggcgctctg tgacctaaga
     1141 ccaaattcct ttaattgcag tcggacaagg tcccggttga tgagactatc gtcgacaacc
     1201 aggataggtt tgtgcccggc atactgccga taagctggcg gttggtggga tggcggtggg
     1261 cagacaatgc tcattttcgg ttccggacgg atggaaagtg gcagacggaa ccaaaaggtt
     1321 gagccatttc cctccatgga ttcataacca atctccccct gcataaggcg gacaagccgc
     1381 tgggagattg caagccccag accggaaccg ccgtgtttgc gtgtgttcgt gccatctgcc
     1441 tgcgtaaacg gttcgaagag ataaggacgt agcccatcag gtatgccgct cccggtatcc
     1501 tggacgctca gtgttgccat gaggacgttc gaatggacac aagtaccgga aaggctaatg
     1561 gtaatcgctc cagctatttc ggtgaatttg atggcattac tgatgagatt acttagaacc
     1621 tgtcgtatcc gacctgcatc tcccaccaga gtttcgggaa gattcgcatc gacgattagt
     1681 tccacggtga gctgtttttc atttgcctga ggctgaaaca gtctcacggt ttcctgaaca
     1741 gcggcacgca ggctgaatgc ttcagagcgg atcacaattt tcccggcttc gatcttggaa
     1801 aaatcgagga tgtcattcag cagattgagc agatttcggg cggatgcatc aatgatgctg
     1861 gcaaactccc tctgttcctc gttcaggtcc gtattcagca acaattcgcc catccccagg
     1921 atggcattca tcggggtccg gatctcgtga ctcatcgtag caagaaaagt actctttaat
     1981 tgcgaagcct ccaaagcccg gtcgcgtgct tccgccagtt cccggttcgt catggcgatt
     2041 tcttctgcat aatgacgtaa cgattcctgg ttctgacgaa gctggttttc ggtatgga
//
LOCUS       DASTJP010000458        18122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1297701, whole genome shotgun sequence.
ACCESSION   DASTJP010000458 DASTJP010000000
VERSION     DASTJP010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297701"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /gene="tuf"
                     /locus_tag="VFQ23_13200"
     CDS             <1..142
                     /gene="tuf"
                     /locus_tag="VFQ23_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET9907601.1"
                     /translation="EMVMPGDNVNLTVELIVPVALEQGSKFAIREGGLTVGAGVVTKI
                     LE"
     gene            151..459
                     /gene="rpsJ"
                     /locus_tag="VFQ23_13205"
     CDS             151..459
                     /gene="rpsJ"
                     /locus_tag="VFQ23_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET9907602.1"
                     /translation="MTKQKIRIRLKAYDHRVLDQSAKRIVETAERSGAHVVGPVPLPT
                     KTERFTIRRSAFIDKDSHEHYEIRTHNRLIDVLDPDSKTIDMLMRLNLPAGVDIEIKI
                     "
     gene            647..1270
                     /gene="rplC"
                     /locus_tag="VFQ23_13210"
     CDS             647..1270
                     /gene="rplC"
                     /locus_tag="VFQ23_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET9907603.1"
                     /translation="MKGLIGKKIGMTQIFDEKGVAYPVTIIEAGPCYVTQVRLPERDG
                     YSAIQLGFGEIHPKKLAGGELGHLKANEISPLRFLREFRSKETANVGDKLTVDVFSVG
                     ENVDVIGTSKGKGFAGSVKRHHFKGGKKTHGQSDRHRAPGSRGAGTTPGRVYKNARGA
                     GHMGNERVTVQALKVVLVDAERNLLGVHGAVPGGKGGLVVIKEARKQ"
     gene            1298..1927
                     /gene="rplD"
                     /locus_tag="VFQ23_13215"
     CDS             1298..1927
                     /gene="rplD"
                     /locus_tag="VFQ23_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET9907604.1"
                     /translation="MKVDVLNVEGKKLREVDLPSAIFEAPINIDLMHQAYVRQMANAR
                     LGTHDTKVRGEVAGGGAKPWKQKGTGRARQGSRRAAQWVGGGRIHTPHPRSYEQRMPK
                     KMRQAALRSALSAKAAEAGVVVVDEFLLSEVKTRVMAGALKNLVGDSTALVVLPEKDQ
                     AYESVMRSTDNLADAKVLLAKYLNIRDLFGYDKLVLPVKTLDALAAFLG"
     gene            1936..2238
                     /gene="rplW"
                     /locus_tag="VFQ23_13220"
     CDS             1936..2238
                     /gene="rplW"
                     /locus_tag="VFQ23_13220"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET9907605.1"
                     /translation="MTTIYDVLRRPLVTEKSSYQSGKLNQYSFIVADSASRTQIKDAV
                     ETLYDVTVVSVNVMNTPAKRGRRLRSRRLLVRKPGHKKAIVTLAEGQTLEIFEGVQ"
     gene            2238..3074
                     /gene="rplB"
                     /locus_tag="VFQ23_13225"
     CDS             2238..3074
                     /gene="rplB"
                     /locus_tag="VFQ23_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET9907606.1"
                     /translation="MAVKKYKPVTPGTRGMTGYTFEEITKSKPERSLLVPLRKSGGRN
                     SYGRVTVRHRGGGHRRFIRVVDFKRDKREISARVAAIEYDPNRTARLALLHYADGEKR
                     YIVAPVDLRVGDSVMSGAGAEIRSGNALPISNIPVGTLIHNIEVKAGRGGQMVRSAGG
                     AAQLLAKEGDFAQIRMPSGEVRLIHQVCYATIGQIGNLDHGNVKLGKAGRKRHKGIRP
                     TVRGSAMSPRDHPHGGGEGRQPIGLAGPKSPWGKPTLGHKTRRNKKTDQLIVRRRSKK
                     SR"
     gene            3093..3374
                     /gene="rpsS"
                     /locus_tag="VFQ23_13230"
     CDS             3093..3374
                     /gene="rpsS"
                     /locus_tag="VFQ23_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET9907607.1"
                     /translation="MSRSLKKGPFIEPKLLKRIEAMNQKGEKKVLRTWSRASVIFPQM
                     VGHTIAVHDGRRHVPIYITENMVGHRLGEFAPTRTFRGHMAAEKAATAG"
     gene            3388..3729
                     /gene="rplV"
                     /locus_tag="VFQ23_13235"
     CDS             3388..3729
                     /gene="rplV"
                     /locus_tag="VFQ23_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET9907608.1"
                     /translation="MQDIQAHLRFLPMSAQKVRLVVDLVRGKNANDALEMLRFVHKAA
                     ALPVRKLLASAVANAEENYGVSRDDLFVAKIFADEAPTRKWRRFGARGRFKPILRRNS
                     HVTVVLRERGS"
     gene            3747..4496
                     /gene="rpsC"
                     /locus_tag="VFQ23_13240"
     CDS             3747..4496
                     /gene="rpsC"
                     /locus_tag="VFQ23_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET9907609.1"
                     /translation="MGRKVHPIGMRLGINQGWQGRWFAEGSAYRQQLHQDFDIRSLLQ
                     GIESKGGAAKSAKVRVLRKDLPDTVKNGKAGISRIEVERYPGKIRIAIHTAKPGILIG
                     RKGEGVKKIRLALETLVGKKVDLDIKEVGSPDTDATLVARNVADQLERRIAYRRAMKR
                     AIQQAMRQGALGIKIMVGGRLGGAEMSRDVWLRDGRVPLQTLRANVDFAKTEAHTTYG
                     QIGVKVWIYKGEAVPETEEKAEATEGVYVSE"
     gene            4528..4953
                     /gene="rplP"
                     /locus_tag="VFQ23_13245"
     CDS             4528..4953
                     /gene="rplP"
                     /locus_tag="VFQ23_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET9907610.1"
                     /translation="MLMPKRVKWRKQMRGRMRGKALRGAEVSFGEFGLQALEPGWVTA
                     RQIEAARRTIVREMKRRGKVWIRIFPAKPYTHKPPETRMGSGKGNVEYYVAVVKPGRI
                     MFEVGGLDAETAVQALKNAQYKFSIKTKVVARHDAGELA"
     gene            4950..5171
                     /gene="rpmC"
                     /locus_tag="VFQ23_13250"
     CDS             4950..5171
                     /gene="rpmC"
                     /locus_tag="VFQ23_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET9907611.1"
                     /translation="MKSVKPSELRNLKSGEIETKLADSREELMKLRFQQVSGQLTDSS
                     RIRILRREIARMQTILREQVAAETVEGEA"
     gene            5168..5491
                     /gene="rpsQ"
                     /locus_tag="VFQ23_13255"
     CDS             5168..5491
                     /gene="rpsQ"
                     /locus_tag="VFQ23_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET9907612.1"
                     /translation="MNNRRRMTGVVTSNKMTKTVVVEITRVFRHPLYRKVVHSSKRVK
                     AHDEIGCQIGDQVQIVESRPLSRDKRWVVENIVKHEIRTADTGVADLKVETVVEETEA
                     SDDSA"
     gene            5478..5849
                     /gene="rplN"
                     /locus_tag="VFQ23_13260"
     CDS             5478..5849
                     /gene="rplN"
                     /locus_tag="VFQ23_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET9907613.1"
                     /translation="MIQHESRLKVADNSGARELLVIHVLGGSTRRYGSIGDVVVASVK
                     SASPQGGVKKSEKVRAVIVRCAKEWRREDGSYIRFDDNAAVILDADGVNPRGTRIFGP
                     VARELREKGFMKIVSLAPEVL"
     gene            5868..6200
                     /gene="rplX"
                     /locus_tag="VFQ23_13265"
     CDS             5868..6200
                     /gene="rplX"
                     /locus_tag="VFQ23_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET9907614.1"
                     /translation="MKVKIRKGDTVEIISGRLEDKGKRGEVINVLLENRRVVVQGVNI
                     RTKHQKQVQTQAGRNLNPGRIQFEGPIAISNVMLICPKCNEPTRVGVQRDEDGARRVC
                     KNCQALID"
     gene            6218..6766
                     /gene="rplE"
                     /locus_tag="VFQ23_13270"
     CDS             6218..6766
                     /gene="rplE"
                     /locus_tag="VFQ23_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET9907615.1"
                     /translation="MNRMQEKYNQEVVPALRKAFDFKNIMQVPRINKVVVNIGMGEAL
                     DNPKSLESAVSDLTIITGQKPVMTKARKSIANFKLREGRLIGTKVTLRGDRMWAFLDR
                     LMNTALPRVRDFRGVSANAFDGRGNYTLGLRDQLIFPEIEYDKIDKLRGMEVTIVTTA
                     KDDDQARVMLQLLGMPFSKKEA"
     gene            6769..6951
                     /locus_tag="VFQ23_13275"
     CDS             6769..6951
                     /locus_tag="VFQ23_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET9907616.1"
                     /translation="MAKKAMQYREERRQHATQVRNRCVRCGRPRGYIRRFGLCRICFR
                     ELALQGKIPGVVKSSW"
     gene            6970..7383
                     /gene="rpsH"
                     /locus_tag="VFQ23_13280"
     CDS             6970..7383
                     /gene="rpsH"
                     /locus_tag="VFQ23_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET9907617.1"
                     /translation="MTVIDPIADMLTRIRNAVMAGHTLVAMPSSKLKVEIAKIMKDEG
                     YLEGFELVENEESSQKVLRMKIKYVGERRARRSVISGIERVSKPGRRVYTKKQDIPWV
                     LSGIGVAILSTPKGVMTGARARQLGVGGEILCKVW"
     gene            7400..7948
                     /gene="rplF"
                     /locus_tag="VFQ23_13285"
     CDS             7400..7948
                     /gene="rplF"
                     /locus_tag="VFQ23_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET9907618.1"
                     /translation="MSRIGRLPVEVPNGVQVNVQGSDVHVKGPKGELKRTFSNLIEIA
                     LENGQVVIKRQSDNPTERALHGTTRAVIANMVRGVSSGFELVLQVDGVGYRSEMQGKD
                     LALFVGYSHPIKVEPPAGISFEVDQKTRQIKVLGFDREVVGQTAAEIRRVRPPEPYHG
                     KGIRYLNEKIRRKAGKAGKGAK"
     gene            7951..8316
                     /gene="rplR"
                     /locus_tag="VFQ23_13290"
     CDS             7951..8316
                     /gene="rplR"
                     /locus_tag="VFQ23_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET9907619.1"
                     /translation="MAKNSRSIGRARRHGRVRKQLAGTAERPRLNVFRSLSAIYAQVI
                     DDQSGHTLASASTVDGDLREQMKGLKKSEQAKLVGQTVAERAKTKDIESVVFDRGGYR
                     YIGRIKALADGAREGGLKF"
     gene            8334..8885
                     /gene="rpsE"
                     /locus_tag="VFQ23_13295"
     CDS             8334..8885
                     /gene="rpsE"
                     /locus_tag="VFQ23_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET9907620.1"
                     /translation="MAEFDKKTEQQGFEGQDQFEERVIEIARVAKVVKGGRRFQFRVT
                     VVVGDKKGTISMGVGKANAVPDAMRKASERARKQMQVVNLAGTTIPHEVLGKVAGARV
                     MLKPASPGTGVIAAGGVRAVLEVAGVRDILTKSQGSANVLNVVQATFDALGQLKSPQE
                     EAARRGKNVKDLLPFWERRKQNA"
     gene            8878..9075
                     /gene="rpmD"
                     /locus_tag="VFQ23_13300"
     CDS             8878..9075
                     /gene="rpmD"
                     /locus_tag="VFQ23_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET9907621.1"
                     /translation="MPNKDTADKVIRVTLVRSPIGYTKDQKATVLALGLRRMHQTIEH
                     RDNPALRGMIQKVIHLVQVEE"
     gene            9081..9536
                     /gene="rplO"
                     /locus_tag="VFQ23_13305"
     CDS             9081..9536
                     /gene="rplO"
                     /locus_tag="VFQ23_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET9907622.1"
                     /translation="MKLHDLVPNQGSKKNRKRVGRGISAGQGKTAGRGTKGEGARSGS
                     GGKAYRQGGNLPFYRRLPFMRGQGFTPRNQVEYNEVNLDQLKESFKADAEVTPEALEK
                     AHLLRDSRNPVVILGRGEITVALKVRAHRVSAGAKEKIEKAGGSVELIS"
     gene            9560..10945
                     /gene="secY"
                     /locus_tag="VFQ23_13310"
     CDS             9560..10945
                     /gene="secY"
                     /locus_tag="VFQ23_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9907623.1"
                     /translation="MPEKKQSAWRFLWRSEDIRRKILITLGILILFRIAANIPVPGAD
                     REALAAFFQAQGGQGGFIGFLNLLSGGTIANFSLLSMGVYPYITAQIILQLLIPIIPA
                     LQRRMQEDQREGRRWMEKWTYYLCVPMAMLSAIGQINIFNSLSLQAIGREIIAFGFSQ
                     NSWLQSTMILLVMTAGTMFAIWLGELISEYGIRGQGLSLVIFAGIVSQIPANIQSLLL
                     DEQNRWILLSIMILVIVLTVIAIVYVQQGRRNVPIMYPHKSQVFYAMQRGKSVKMPQP
                     ILPLMVNLSGMIPLIFASAILQFPAIVASYFVRNESERVSAIATSIQNFFNATGTNSW
                     GYWALYFLMVVAFTYFYTTVIFEQQNYGDNLKKSGAQIPGVHSGAATQKYLSRIQSRI
                     TLPGALLLGVVAIMPFLLQLILPAAANSVGLFLVTSSGLLIVVGVVRDTFMNIEAELK
                     LHGYQEKLLIS"
     gene            10954..11604
                     /locus_tag="VFQ23_13315"
     CDS             10954..11604
                     /locus_tag="VFQ23_13315"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET9907624.1"
                     /translation="MTTFIVLLGPPGVGKGTQAEILSKKTGLAHISSGDLFRENLKNQ
                     TELGKLAQTYMHEGELVPDDVTIAMIRDRLHRPDCKEGAILDGFPRTPTQAAALEYML
                     EEFNGQVDTVPVIIVADDELVERLSHRWTCRASGHIFNDKSNPPEVAGKCDFDGSELY
                     QRDDDKAETVKHRIQVYMDQTLPLISYYRNLGKLVEVDGMQPIDDVTFELMKVLKK"
     gene            11609..12379
                     /gene="map"
                     /locus_tag="VFQ23_13320"
     CDS             11609..12379
                     /gene="map"
                     /locus_tag="VFQ23_13320"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9907625.1"
                     /translation="MSWARQIHLKSSAELQIMREAGRINASVLAAVRECIKPGVSTAD
                     LNAAAEEVLKKHGCVSPFKGYGHPPFPTSITASINQELVHGIPSKKRKLKAGDIVSID
                     CGTVLDGFVADSAFTAGVGEISHTAQELLDVTEGALKAAIQVMCVGNRTGDVSAAIQQ
                     YVESRGYYVTREYTGHGVGRQMHEGPQVPNYGKPGTGLPLKAGMTIAIEPMVLVGTAE
                     TRVLPDEWTVVSADGSLTAHFEHSVAVTEGEPLLLTVP"
     gene            12491..12604
                     /gene="rpmJ"
                     /locus_tag="VFQ23_13325"
     CDS             12491..12604
                     /gene="rpmJ"
                     /locus_tag="VFQ23_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9907626.1"
                     /translation="MKVSPSIRKRCAKCRIIRRKGIVFVICENPKHKQRQG"
     gene            12611..12997
                     /gene="rpsM"
                     /locus_tag="VFQ23_13330"
     CDS             12611..12997
                     /gene="rpsM"
                     /locus_tag="VFQ23_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9907627.1"
                     /translation="MARIEGVDLPRNKRVEIGLTYLFGIGPTRAQAILTHTKVNPDTR
                     VKDLSEADVAAIREYISKNYKVEGDLRREVQMSIKRLIEIGSYRGMRHRRNLPARGQR
                     TRTNSRTRKGTKKTVAGRGRRRGAKK"
     gene            13012..13416
                     /gene="rpsK"
                     /locus_tag="VFQ23_13335"
     CDS             13012..13416
                     /gene="rpsK"
                     /locus_tag="VFQ23_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9907628.1"
                     /translation="MAQSVRSTRRNTGAKKARRAISAGQVHIFASFNNTIVTVTDTEG
                     NTVAWGSAGSAGFKGSRKSTPFAARLAAEQAIKAAQQLGIQEVDLFVKGPGPGRENAI
                     RAVQAMGLRVRSIADITPVPHNGCRPPKKRRV"
     gene            13440..14090
                     /gene="rpsD"
                     /locus_tag="VFQ23_13340"
     CDS             13440..14090
                     /gene="rpsD"
                     /locus_tag="VFQ23_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET9907629.1"
                     /translation="MSKNLNPVCKLCRREGEKLYLKGERCFTPKCSFERRSFAPGQHG
                     RTGSRGGGNRTGRESDYLRQLRAKQRARRVYGVLERQFRRYYEIANQRLGITGTNLLQ
                     ILESRLDNVVYRLGFASSRAQARLLVTHGHFTVNGRRTDVPSMLLRDGDKVAVRDGSR
                     GLEYFKVLSDLADARTSPSWLDRDAKQLSGAIQRLPERTEIDGSLNEQLIVEYYSR"
     gene            14158..15126
                     /locus_tag="VFQ23_13345"
     CDS             14158..15126
                     /locus_tag="VFQ23_13345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET9907630.1"
                     /translation="MTGITNMVMPKIEREAEARNYGKFVISPLERGYGVTLGNALRRV
                     LLSSLEGAAVSSIRIADVLHEFSEIPGVREDVIQVTLQVKQLRLKLDGVDSTRMHLEV
                     RGEGTVTAADIITPAEVEVVNPELYLFTVDNPKTKLDLEFTVERGRGYSPANERSGHM
                     PIGELPIDAIFSPVKRVNWEVTSARVGQSTNYDKLTLEIWTDGTVSPERSLSTAAKLI
                     IDHLRYIAGVSEETLAISIEKEVGGSRITSELADTPVEALDLSVRVFNSLKRTGITTV
                     GDVLDLLEKGETAVMSIRNFGEKSLDELRQKMREKGYMKDESSSAE"
     gene            15133..15510
                     /gene="rplQ"
                     /locus_tag="VFQ23_13350"
     CDS             15133..15510
                     /gene="rplQ"
                     /locus_tag="VFQ23_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET9907631.1"
                     /translation="MRHQVSGYRLGRTTGARLALRRNLIKQFFTHERIQTTRAKAAAI
                     RGEAERLITIARNSADGSDIEKVNARRLVISKLGDNQVIKRLFDEIAPRFANRNGGYT
                     RVLKLGPRMGDSAEMVILELVEE"
     gene            15562..16326
                     /gene="truA"
                     /locus_tag="VFQ23_13355"
     CDS             15562..16326
                     /gene="truA"
                     /locus_tag="VFQ23_13355"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET9907632.1"
                     /translation="MARYQLTLVYDGTDFFGSQRQAKSRTVQGELEKALNKLGWIGRS
                     VILSGRTDTGVHATGQVASFDLDWSHSEAKLIGALNSELPPDLSVQTARIVHDKFHPR
                     FDATSRSYQYRLFCQPIRDPIREHFAWRVWPAINPDALGHFAKLFLGTHDFSAFGSRT
                     TPKGGTVRTVVKAEWTQTGEAKDEWRFEVQADAFLYRMVRKLVFVQVAAAQGKVLAET
                     ITSALVKPATAKIPAGLAPAHGLTLVQVTYDESIGK"
     gene            16349..16801
                     /gene="rplM"
                     /locus_tag="VFQ23_13360"
     CDS             16349..16801
                     /gene="rplM"
                     /locus_tag="VFQ23_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET9907633.1"
                     /translation="MYKSYYPKASEIDRKWVLVDANGQTLGRIATRIAHVLLGKHKPS
                     FTPGVEMGDFVVVINAERVTVTGTRTSTKMETKMYHRHSNYPGGYKAISLRDQLQQHP
                     DRVLRAAVWGMLPHNRMGRALLKRLKIYAGPSHPHGTNNPEPLATPQE"
     gene            16819..17214
                     /gene="rpsI"
                     /locus_tag="VFQ23_13365"
     CDS             16819..17214
                     /gene="rpsI"
                     /locus_tag="VFQ23_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9907634.1"
                     /translation="MSVQYYEAVGRRKESTARVRVMGGSGRFIVNDKEATVYFSRLGD
                     LHDILRAFTAVGQDSKKYDITVKVNGGGVTGQTEAVRLGVARALVLLNGEWISALRKN
                     GLLTRDARIKERKKPGLKKARKAPTYTKR"
     gene            17297..>18122
                     /gene="mazG"
                     /locus_tag="VFQ23_13370"
     CDS             17297..>18122
                     /gene="mazG"
                     /locus_tag="VFQ23_13370"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET9907635.1"
                     /translation="MDLSLIAKVFDALGFSPPSRLILLEAETLSSAHVPSFPPDIPAL
                     LIGVDSPDMALHLKTVLLTTYPAEHIVHIFQNGMTKEERLNDFEFSENSCIYVPPLAE
                     GTSFESFAEIIAHLRAPNGCPWDREQTHETLRKHLLEESYETIAAIDSGDFKSMREEF
                     GDLLLQVVLQAQIANEAGQFNVNQVIQGIYSKIIRRHPHVFGDLKLEGVEGVLANWEK
                     LKEQERGEKKDGKGLLDGVPGALPALSQAQEYQDRAARVGFDWPEIEGVLDKVKEEI"
BASE COUNT         4612 a         4135 c         5042 g         4333 t
ORIGIN      
        1 ggagatggtg atgccgggtg acaatgtgaa cctgaccgtc gagttgatcg tgcccgtcgc
       61 tctcgagcag ggatccaagt tcgccatccg tgaaggtggt ctcaccgtcg gcgcgggtgt
      121 cgttaccaaa atattggaat aggtgcaggc atgaccaaac aaaaaatccg tattcgcctc
      181 aaggcttatg accatcgtgt gttggatcag tctgcaaaac gaattgttga gacagcagaa
      241 cgaagtggtg cacacgttgt gggtcctgta cccttgccaa ccaagaccga acggtttaca
      301 attcggcgct ctgcattcat agataaagac tcacacgagc actatgaaat tcgtacacac
      361 aatcgattga ttgatgttct agatccagac tcaaagacaa tcgatatgtt gatgcggctt
      421 aatctgcctg ctggtgtaga tattgaaata aagatttagt tcgtctttag agagttgaga
      481 acgtaattat tgctctctag tttcgacaat acaagaacgg ttccctccgg gataacaaaa
      541 acacttagca tcccgacaaa ccgtttactg tgttgcctgg agatgatgca gccgaagccc
      601 cggctgacag tctcataaat tcttttgaag gagaaaaccg agtatgttga aagggcttat
      661 tggaaagaag attggcatga cccagatctt cgatgagaaa ggtgtcgcat accctgtgac
      721 aatcatcgaa gctgggccat gttacgttac acaggtccgt cttcccgaac gcgatggata
      781 ttcagccatc caactggggt ttggtgaaat tcatcctaaa aaacttgctg gtggtgagtt
      841 agggcacttg aaagcaaatg aaatatcacc tctgcggttt ttgcgagaat ttcgttcgaa
      901 ggaaactgcc aatgtggggg ataagctgac agtagatgta ttttccgttg gtgaaaatgt
      961 tgatgtgatc ggaaccagca agggcaaggg ttttgcaggt tcggtcaagc gtcatcattt
     1021 caaaggcgga aagaagaccc atggccaatc tgatcgtcat cgtgcaccgg gctcacgcgg
     1081 cgctggaaca acaccaggcc gtgtctataa gaatgcacgt ggagctgggc acatgggaaa
     1141 cgaacgcgtt acagtccagg cactcaaggt ggttttggtt gatgctgaac ggaatttgct
     1201 tggcgtacat ggcgctgttc ccggtggcaa aggcggactg gttgttatca aagaagctcg
     1261 aaagcaataa tgctgaggaa attggagcta ggtaatcatg aaagttgatg ttctgaatgt
     1321 agaaggtaag aagcttcgtg aggttgatct tccctcagcg atctttgagg cccccattaa
     1381 tatagatttg atgcatcagg cttacgtgcg ccaaatggca aatgcacgcc ttggcacgca
     1441 tgatacgaaa gtcagaggtg aagtggcagg tggcggcgcg aagccctgga agcaaaaggg
     1501 aacgggacga gcccgtcagg gttctcgccg agccgctcag tgggttggtg gtggacgtat
     1561 tcatacacca caccctcgca gttatgagca gcgcatgccc aagaagatgc gccaggccgc
     1621 actacgatca gctctgtctg ccaaggctgc tgaggctgga gtcgttgtgg tggacgagtt
     1681 cttgctctcc gaagtcaaga ctcgcgtcat ggctggtgcc ctcaagaatc tcgtaggcga
     1741 ctccaccgct ctggttgttt tacctgaaaa ggatcaggct tacgagagtg tcatgcgctc
     1801 aacagataat ctggcagacg caaaagtttt gctggcgaaa tatttgaaca ttcgggattt
     1861 gttcggatat gacaagctgg ttttaccggt caaaacgctg gatgcccttg cggcgttctt
     1921 aggataggtg aggcaatgac tactatttat gatgtcctcc gccgccccct ggtaactgaa
     1981 aagtccagtt accaatctgg caaattaaat cagtattcct ttattgtggc cgatagtgcc
     2041 tcgcgaacgc agattaaaga cgctgtcgaa acactctatg atgttacggt tgtgagtgtg
     2101 aatgtaatga acacaccagc caagcgaggg cgccgtcttc gtagtcgccg cctgctcgtt
     2161 cgaaagcccg gacacaagaa ggctatcgtt acgctggctg aagggcagac acttgaaatt
     2221 tttgagggag tgcagtaatg gctgttaaga agtacaaacc ggtaactccc ggcactcgcg
     2281 gaatgaccgg ttacacattt gaggaaataa ccaagtccaa accggaacga tctttactgg
     2341 tgccgcttcg caagagtggc ggacgcaact catacggacg cgttaccgtc cggcatcgtg
     2401 gtggggggca ccgccggttt atacgtgttg tggatttcaa acgcgacaag cgcgagattt
     2461 ctgccagggt cgcagcgatt gagtacgatc cgaaccgcac cgcacgactt gccctgttgc
     2521 actatgccga tggcgagaag cgttatatcg tggcaccagt cgatctcaga gtgggtgatt
     2581 cagtcatgtc gggcgcaggt gccgagatcc gctccggaaa tgcccttccg atttccaaca
     2641 tccctgttgg taccttgatc cacaatattg aggtcaaagc ggggcgtggt ggtcagatgg
     2701 tccgttcagc gggcggggct gcacaattgc ttgccaagga aggtgatttt gcacagatcc
     2761 gaatgccttc tggcgaagta cgcctgattc accaggtttg ttacgcgacc attggtcaga
     2821 tcggaaatct tgatcatgga aatgtcaaac ttggaaaggc tggccgaaaa cgccacaaag
     2881 gtattcgccc aacggttcgc ggctcagcga tgagtccacg cgatcatcct catggtggtg
     2941 gtgaaggacg tcaaccgatt ggcttggccg gtccgaagag tccgtggggt aaacccaccc
     3001 tgggtcataa gacccgtcgt aacaagaaaa ctgatcagct tattgtgcgt cgtcgcagta
     3061 agaagagccg ctaattcgta tagaggtacg taatgtcacg atcattgaaa aaaggcccgt
     3121 ttatcgagcc caaactgttg aagagaattg aagcgatgaa ccaaaaaggt gagaagaagg
     3181 ttttgcgaac ctggagtcgc gccagcgtga tcttcccaca aatggttggt catacaattg
     3241 ccgtccatga tggacgccga catgtcccga tctatatcac cgaaaacatg gtcggtcatc
     3301 gattgggcga gtttgcgccc acgcgaacat tccgcggcca catggctgct gagaaggccg
     3361 caacggctgg ttagaggaga gtaaatcatg caggatattc aagcccattt gcgcttcctc
     3421 ccaatgtcgg cccaaaaggt tcgcctggtt gtcgatttgg tgcgcggcaa aaatgcgaac
     3481 gatgcgctgg agatgcttcg ctttgtccac aaggcagccg ctttaccggt ccgaaaattg
     3541 ctggcttcgg ctgttgcgaa tgctgaggag aattatggcg ttagccgcga tgatttgttt
     3601 gtcgcgaaga tttttgctga cgaggccccg actcgcaagt ggcgtcggtt tggcgcacgc
     3661 ggtcgtttca agccgatact ccgccgaaat tcccacgtga ctgtggttct gcgcgagcgc
     3721 gggtcatagg aaggtaagga gaaatcatgg gtcgtaaagt tcatccaatt ggtatgcgtc
     3781 ttggaatcaa ccagggctgg cagggacgct ggtttgctga gggttcagca tatcgccagc
     3841 aattacatca ggattttgac atccgtagct tgttacaggg aatcgagtca aaaggcggcg
     3901 cagcaaaaag tgcgaaggtt cgcgtgcttc gcaaagacct gcctgatacc gtcaagaatg
     3961 gcaaagcagg aatttctcgt atcgaagttg agagatatcc gggcaagatt cgaattgcta
     4021 ttcatacagc gaaaccgggt attttgatcg gccgtaaagg cgaaggcgta aagaagattc
     4081 gtctggcgct tgagacactt gtcggtaaaa aggtggatct tgatatcaag gaagttggct
     4141 caccagatac cgatgctaca ctcgttgcac gaaatgttgc agatcagctt gagcgacgta
     4201 ttgcataccg ccgtgcgatg aagcgcgcaa ttcaacaggc aatgcgtcag ggcgccctgg
     4261 ggatcaaaat tatggtagga ggtcgtttgg ggggcgcaga aatgtcgcgc gatgtctggc
     4321 ttcgtgacgg acgcgtcccg ctgcaaactt tgcgtgcaaa tgtggatttt gcaaaaactg
     4381 aagcgcatac aacctatgga cagatcggcg tgaaagtttg gatttataag ggtgaggcag
     4441 tacctgagac tgaagaaaaa gcggaggcaa ctgaaggcgt atacgtgagc gaatagcccg
     4501 cggttgtctt ttgaagaggt agttactatg ttgatgccaa aacgtgtgaa atggcgcaag
     4561 caaatgcgcg gtcgaatgag gggcaaggcc ctgcggggcg ccgaggtctc cttcggtgag
     4621 tttggtctgc aggcgcttga acctggttgg gtaacagccc gacagatcga ggcagctcgt
     4681 cggacgatcg tccgcgaaat gaagcgtcgc ggtaaggtat ggattcgcat tttccctgcc
     4741 aagccctata ctcataagcc acctgagact cgtatgggtt ccggtaaagg caatgttgag
     4801 tactatgtgg cagttgtgaa acccgggcgt attatgtttg aggtcggggg gctggacgca
     4861 gagactgcgg tgcaggcact caagaatgcg caatataaat tttcgatcaa gactaaggtt
     4921 gtggcccgtc acgatgcagg agaactggca tgaagtccgt aaaaccaagc gaattgcgca
     4981 acctgaagtc aggtgaaatt gaaacaaagt tggcggactc acgagaagag ctgatgaaac
     5041 ttcgctttca acaggttagc ggccaactga cggacagcag ccgtataaga attttacgcc
     5101 gcgagatcgc cagaatgcag accattcttc gtgaacaagt tgcggctgaa actgtagaag
     5161 gtgaagcatg aataatcgtc gtcgcatgac tggtgtggta accagcaaca aaatgaccaa
     5221 gacggtagtc gtcgaaatca cgcgtgtatt tcgccatccg ttgtatcgta aagtggttca
     5281 ctccagcaag cgagtgaagg ctcatgatga aattggctgc cagatcggcg atcaggttca
     5341 gatcgtagag tctcgaccac tttcccgtga caagcgctgg gtggtggaaa acattgtcaa
     5401 gcatgagatt cgaactgccg atacaggtgt cgcggatttg aaggtggaaa cggtggttga
     5461 ggagacggag gcaagcgatg attcagcatg agtctcgtct taaagttgct gataattccg
     5521 gagcccgcga actgttagtc atccatgtgc tgggtggctc cacccgtcgt tacggaagca
     5581 ttggtgacgt ggtcgttgcc tccgtgaaat ctgcatcacc gcaaggtggt gtgaagaaaa
     5641 gcgaaaaagt acgcgctgtc attgtgcgtt gtgctaaaga gtggcgacgt gaagatggct
     5701 cttatattcg ttttgatgac aatgcggccg tcatattgga tgctgatggg gtgaacccca
     5761 gaggcacccg tatttttgga cctgtcgcca gagagcttcg tgaaaaaggc tttatgaaga
     5821 tcgtttcact tgctccggaa gtgctgtagg cggaggagta tttagatatg aaagtcaaga
     5881 ttcgtaaagg tgatacagtg gaaatcatca gcggacgcct ggaagataag ggcaaacgcg
     5941 gtgaggtcat caacgtatta cttgaaaatc gacgcgtggt ggttcagggt gtaaacatcc
     6001 gcaccaagca tcagaaacag gtacagaccc aggcgggtcg taatctcaac ccgggccgga
     6061 tccagtttga agggccaatt gccatctcaa acgtaatgct gatctgcccg aaatgcaatg
     6121 agccaacacg tgttggtgtt cagcgtgatg aagacggtgc tcgtcgtgtc tgtaagaatt
     6181 gtcaggcact gattgattag acgcggagta attatcgatg aatagaatgc aggaaaaata
     6241 taaccaggaa gtggtgccgg ccctgcgcaa ggcatttgac ttcaaaaaca tcatgcaggt
     6301 tccgcgaatc aataaagttg tggtgaacat tggcatgggt gaggcgttgg ataatcccaa
     6361 gtctctggaa tcggctgttt ctgatctgac cataatcaca ggtcaaaaac ccgtgatgac
     6421 caaagcccgt aagagcatcg cgaatttcaa acttcgcgag ggacgcttga tcggaaccaa
     6481 ggtgaccctg aggggtgacc gcatgtgggc ctttctggat cgcttgatga atacggcgct
     6541 cccgcgtgta cgtgattttc gtggtgtctc tgccaatgcg tttgatggcc gcggcaacta
     6601 caccttgggc ttgcgtgatc aattgatctt tccggagatc gaatatgaca aaatcgacaa
     6661 actgcgcggc atggaggtca caattgtgac cactgcaaaa gatgatgacc aggcacgtgt
     6721 aatgctacag ttgctcggca tgccgttcag caagaaggaa gcgtaaatat ggcaaagaaa
     6781 gctatgcaat atcgagaaga acgtcgccag catgcaactc aggtacggaa tcgctgtgtt
     6841 cgttgtggtc gcccgcgcgg ctatattcgt cgtttcggtt tgtgccgaat atgcttccgt
     6901 gaactggcat tgcagggcaa gatccctggt gtcgtgaaat cttcttggta accaggtaga
     6961 ggtaatcgaa tgacagttat agatcctatc gctgatatgt tgacccgcat acgcaatgcg
     7021 gtgatggctg gccatacact ggttgcgatg ccaagctcga agctcaaggt tgagattgcc
     7081 aagatcatga aagatgaagg ctatctcgaa ggctttgaat tggttgagaa cgaggagtcg
     7141 agccagaagg ttttgcgaat gaaaatcaag tacgttggcg aaagacgcgc ccgccgttcc
     7201 gttatctcgg gaatcgagcg tgtcagcaaa cccggacgac gtgtttacac caagaaacag
     7261 gatatcccct gggtgctttc aggaattggc gttgccatcc tttccacacc caagggtgta
     7321 atgaccggcg cgcgcgctcg acaactgggc gtaggcggcg aaatcctgtg caaggtgtgg
     7381 tagaaggcag gaggaagtaa tgtctcgtat tggacgttta ccagtggaag ttccgaacgg
     7441 tgtacaagtc aatgtgcagg gttccgatgt ccacgtgaaa gggccgaaag gcgaactgaa
     7501 gcgcacattt tcgaacctga tcgaaatcgc cttggaaaat ggtcaggtgg tgattaagcg
     7561 ccaatcagat aatcccaccg agcgcgcgtt gcatggcacg actcgtgccg tgattgctaa
     7621 catggttcgc ggagttagca gtggatttga gcttgtactt caggtggatg gcgttggtta
     7681 tcgctctgaa atgcaaggga aagacctggc tttatttgtc ggttattctc atcccataaa
     7741 agtggaaccc cccgccggga tttcattcga agttgaccaa aagacgcgtc agatcaaagt
     7801 gcttggattt gatcgtgagg ttgttggcca gactgccgcc gaaattcgac gtgttcgacc
     7861 tcctgagcca tatcatggca aagggattcg ctatctcaat gagaagatcc gtcgaaaagc
     7921 cggtaaagct gggaaaggag ccaagtaagc atggcgaaaa attctcgttc cattgggcgt
     7981 gcacgtcggc atgggcgcgt ccggaagcaa ttagcgggaa cagccgaacg tccgcgtttg
     8041 aacgtttttc gcagcctgtc cgcaatctac gcgcaggtga tcgacgatca atcgggtcat
     8101 acattagcat ccgcttcaac ggtggatggc gatttgcgcg agcaaatgaa aggcctgaag
     8161 aaatcggaac aggcaaaatt ggtcggtcag accgttgcag aacgtgccaa aaccaaggat
     8221 attgaatccg ttgtctttga ccgcggcggc taccgctata tcggacgtat caaagcgctt
     8281 gccgatggtg cgcgtgaagg cggcctgaaa ttttaggctg atgtggagta gaaatggcag
     8341 aatttgataa gaagacagaa cagcagggct ttgaagggca ggaccagttc gaagagcgcg
     8401 tgattgaaat cgcccgggtt gccaaggttg ttaaaggcgg ccgccgcttt caatttcgcg
     8461 tcactgtcgt ggtcggcgat aaaaaaggta cgatttccat gggtgttggc aaggccaatg
     8521 ctgtgcccga tgccatgcga aaggcttccg aacgcgctcg caaacagatg caggtcgtca
     8581 acctggctgg cactaccatc cctcatgaag ttttaggtaa agttgccggg gcgcgcgtca
     8641 tgctcaaacc cgcttcacct ggaactggtg ttatcgctgc aggtggtgta cgtgcggtgc
     8701 tcgaagtggc gggcgtccgt gatattttga ccaagtcaca gggcagcgcc aatgttttga
     8761 atgtggttca ggcgaccttt gacgcgctcg ggcaattgaa gtcacctcag gaagaggctg
     8821 ctcgtcgcgg gaagaatgta aaggatctgc tgccgttctg ggagcggagg aaacaaaatg
     8881 cctaataaag ataccgcaga taaagtgatc cgcgtgacgt tggttcgcag cccgatcggt
     8941 tataccaagg atcagaaggc aactgttctg gcgctgggtc tccgccgaat gcatcagacc
     9001 attgaacacc gagacaaccc tgccttgcgc ggaatgattc aaaaggtcat tcacttagtt
     9061 caagtagaag agtagaaacg atgaaattac acgatcttgt acccaatcag ggttctaaaa
     9121 agaatcgaaa acgtgttgga cgtggtatct ccgcgggtca agggaaaacg gccggacgtg
     9181 gtaccaaagg tgaaggcgcg cgctcagggt ctggaggcaa agcctatcgt cagggtggta
     9241 atcttccgtt ctatcgtcgt ctccccttta tgcgcggtca gggattcacg ccacgtaatc
     9301 aggtggagta caacgaagtc aatttagatc aattgaaaga gtcattcaaa gctgacgctg
     9361 aagtcactcc tgaagctttg gaaaaggcac atttacttcg agattcccgc aatccagtgg
     9421 tcatcttagg ccgcggcgag atcacggtgg cgctgaaagt gcgcgctcat cgggtcagtg
     9481 ccggtgccaa ggaaaagatt gaaaaagctg gtggtagcgt ggagttgatt agttagaatg
     9541 aatcgaagag gattttacaa tgcctgagaa gaaacaatcc gcctggcgct ttttatggcg
     9601 atctgaagat atccggagga agatactgat cactctgggt atcttaatcc tgttccgcat
     9661 tgcagcgaat atacctgtcc ccggcgctga ccgtgaagct ctggcagctt tctttcaggc
     9721 tcagggtggc cagggaggat ttattggctt tttgaacctt ctctccggag gcacaatagc
     9781 taacttctcc cttctctcga tgggcgttta tccctatatt actgcccaga ttattttgca
     9841 actgctgatt cccatcatcc cggctttgca gcgccgaatg caggaagacc aacgggaagg
     9901 gcggcgctgg atggaaaaat ggacatatta cttatgtgtg ccaatggcca tgctatcggc
     9961 gatcggtcaa atcaatatct ttaattcgct ctccctgcag gccatcggcc gagaaatcat
    10021 cgcgtttggt ttctctcaaa attcatggct gcaatccacc atgatcctcc tggtgatgac
    10081 ggctggtacc atgttcgcca tctggctggg cgagctgatt tccgaatatg gcattcgtgg
    10141 gcagggtcta tcacttgtta tttttgccgg gatcgtgtct cagattcccg caaatattca
    10201 gagcctgctg ctcgatgagc aaaatcgttg gattttgctg tcgatcatga tcctcgtgat
    10261 tgtcctaact gttattgcga ttgtctacgt tcagcaggga cgccgcaatg tgccgatcat
    10321 gtatccgcat aaaagccagg ttttttatgc aatgcagcgc ggtaaatctg tgaagatgcc
    10381 tcagccaatt ttgccgttga tggtgaatct ctctggaatg atcccactta tctttgccag
    10441 cgcaattttg caatttcccg cgattgttgc aagttatttt gtgagaaatg aaagtgagag
    10501 agtaagcgct attgcaacca gtatccaaaa tttctttaat gccacgggta caaattcctg
    10561 gggatactgg gcattgtatt ttctgatggt ggtagccttt acttatttct ataccacggt
    10621 gatttttgaa cagcaaaatt atggtgataa tctgaagaaa tcaggcgccc aaatccccgg
    10681 cgtgcatagt ggtgcggcaa cgcaaaaata tttgagtcgt attcaaagtc ggattacttt
    10741 gccgggtgct ttattgttgg gagttgtggc gatcatgcca ttcctgttgc aattgatctt
    10801 acccgcggct gcaaattcgg tgggcctatt tttggtgact tcttccggct tgctgatcgt
    10861 tgttggcgtt gtccgggata cctttatgaa tatcgaggcc gaactaaaat tacatggcta
    10921 tcaggaaaag ctgctgatta gctaggagtg taaatgacga ccttcattgt tctcctcggt
    10981 cctccgggtg ttggcaaggg tacgcaggca gaaatattat ccaagaagac tggtttggca
    11041 catatctcat caggcgactt gtttcgcgag aaccttaaaa atcagacaga gcttggaaag
    11101 ctggctcaaa cgtatatgca cgagggcgaa ctggtgccag atgatgtgac gattgcgatg
    11161 attcgggacc gccttcatcg cccggattgt aaggaaggtg ccattctgga tggttttcct
    11221 cgcactccaa cccaggccgc ggcattggaa tacatgctgg aggagttcaa cggtcaagtt
    11281 gacaccgttc cggtgattat tgttgcagat gacgaacttg tggaacggct gagtcatcgt
    11341 tggacctgcc gggccagtgg gcatattttt aatgataaat ccaatccacc cgaggtagct
    11401 ggcaaatgcg actttgatgg atcggagcta tatcaacgtg atgatgataa agctgaaacc
    11461 gtaaaacatc gtatccaggt atatatggat cagaccttgc cgttgatatc ttattatcgc
    11521 aatcttggga aactggttga agtcgatgga atgcaaccca ttgacgatgt aacctttgaa
    11581 ttgatgaaag tgttgaagaa gtaagtttat gagttgggca cgccagatcc atcttaaaag
    11641 ttcggccgaa ttgcagatca tgcgcgaagc tgggcgcatt aacgcaagtg ttctggcggc
    11701 tgtcagagaa tgtataaaac ccggtgtttc tactgccgac ctcaacgcgg ctgctgagga
    11761 agtgctaaag aaacatggat gtgtatcgcc gttcaaggga tatggtcatc caccattccc
    11821 gacttcaatt acggcaagca tcaatcagga actggtccat ggtattccga gcaagaaacg
    11881 gaaactgaaa gccggagata ttgtatcaat cgattgcgga acagttcttg atggttttgt
    11941 agccgattca gcattcaccg ctggtgtggg agaaatatct catacggctc aggaattgct
    12001 ggatgtcacc gaaggtgcat tgaaggctgc gattcaagta atgtgtgtag gtaaccggac
    12061 cggcgatgtt tcagcggcaa tccagcaata tgttgagagc cgcggctact atgtcactcg
    12121 tgaatatacc ggtcacggcg ttggaaggca aatgcacgaa ggacctcagg tcccaaatta
    12181 cggcaaaccg ggtacagggt tgccccttaa agcaggtatg accattgcta tcgagccgat
    12241 ggtccttgtt ggaacggcag aaacacgcgt cctgccagat gaatggacag tcgtttctgc
    12301 agacggatca ctgacagcgc attttgaaca ttctgtggct gtcaccgaag gagaacctct
    12361 actcctgact gtcccatgat ccgcaaattg tttcgtgtaa tctagacgaa aaaaagacga
    12421 acgtgtataa tcaaaaactt ggctacgcat caagttaaag tgaaaatgtg agtttgcaag
    12481 gagcattgta atgaaagttt caccgtcaat acggaagcgc tgcgctaaat gcagaatcat
    12541 caggcgcaag ggtatagtgt ttgtgatctg cgaaaatcca aaacacaaac agcgacaagg
    12601 ataggaagct atggcgagaa ttgaaggtgt cgatctgccc cgcaataagc gggtggaaat
    12661 cggtctgacc tacttatttg ggatcggacc cactcgcgcg caagcgattc ttacgcatac
    12721 gaaggtcaat cctgatacaa gagtgaagga cctttcggaa gcggatgtcg ctgcgatccg
    12781 cgaatatata agcaagaatt ataaagtgga aggcgacctg cgccgtgaag tgcagatgag
    12841 catcaagcga ctgatcgaga tcggttcgta tcgcgggatg cgtcatcgcc gcaatctgcc
    12901 ggctcgcggt cagcgcacac gaacaaattc ccgtactcga aaaggtacga aaaagactgt
    12961 tgccgggcgc ggacgtcgcc gcggcgcgaa gaaataagag gtttagaaat tatggctcaa
    13021 agtgttcgtt ctacgcgtcg aaatacgggt gccaaaaaag ccaggcgcgc catatcggcc
    13081 gggcaggttc atatctttgc ctccttcaac aatacgattg tgaccgtgac cgacacggaa
    13141 ggcaatacgg ttgcgtgggg aagtgcagga tccgctggtt tcaaaggctc tcgtaaaagc
    13201 acaccgttcg cagcacgcct ggcagctgag caggccatta aggccgcaca gcaattagga
    13261 attcaagaag ttgatttgtt cgtcaaaggt cccgggcctg gccgtgaaaa tgcaattcgg
    13321 gccgtacaag ccatgggact ccgagttcgt tcaattgctg acattacacc agtgccgcat
    13381 aacggttgcc gtccgccgaa aaagcgacgc gtgtagttct atttagagga aagtaattta
    13441 tgtcgaagaa tcttaatccg gtttgtaaac tttgccggcg cgaaggcgaa aaactctatt
    13501 tgaagggtga gcgttgcttc actccaaagt gttcgtttga aagacgcagc ttcgcaccgg
    13561 gacaacatgg acgcacggga agccgcggtg gtggtaaccg aactggccgc gagtctgact
    13621 atttgcgcca gttgcgcgcc aagcaacgcg ctcgccgcgt ctatggtgta ttggaacgtc
    13681 agttccgcag atattatgaa atcgccaatc agcgcctcgg tatcacgggc acaaatctgt
    13741 tgcagatttt ggaatcgcgc ctggacaacg tcgtctaccg acttgggttt gcctccagcc
    13801 gcgcacaggc acgtttactt gtgacccatg gtcattttac agtaaacggt cgccgcacag
    13861 atgtcccctc gatgcttctc agagacggtg acaaagtcgc cgtacgagat ggatcgcgcg
    13921 gtttggaata cttcaaagtg ttaagtgatc tggcagatgc tcgcacttca ccgagttggt
    13981 tggatcgcga tgcgaagcag ttgtcaggtg ccatccagcg tcttcctgaa cgcactgaaa
    14041 tcgatggcag tttgaatgaa cagctcatcg tcgaatacta ctcacggtag tagttggttt
    14101 tcctttggct ggtgttccag cgaagtttat tctgtttcta acagggagag gtgaaccgtg
    14161 actggtatca cgaatatggt tatgccgaaa atcgagcgcg aggctgaggc tcgtaattac
    14221 ggaaaatttg ttatcagccc actggagcgc ggttatggcg ttaccttagg taatgcatta
    14281 cgccgggtgc ttctttcttc attggaggga gcagcggtct catccatccg tattgcggat
    14341 gttttgcatg agttcagcga gatcccgggt gttcgcgaag atgttatcca ggtgacactg
    14401 caagtcaaac aattgcgact gaaattggat ggtgttgata gtacccgtat gcacctggaa
    14461 gtccgtggcg aaggcaccgt gactgcagca gatatcatca cacctgcaga ggttgaagtt
    14521 gtcaacccgg agctttattt atttactgtg gataacccga agaccaagct tgatcttgaa
    14581 ttcacagtgg agcgcggtcg cggctattcg ccagccaacg aacgcagcgg gcatatgccg
    14641 attggcgagt tgccgattga tgccatcttc agcccggtca agcgcgtgaa ttgggaagtg
    14701 acgtccgcac gagtgggtca aagcacgaac tatgacaagc tgaccctgga gatctggacg
    14761 gatggcacgg tttctccgga aagatctctt agcaccgccg ctaaattgat cattgaccac
    14821 ctgcgatata ttgccggagt tagtgaagaa accctcgcca tttccatcga gaaggaagtg
    14881 ggtggcagcc gcatcaccag cgaactggct gacacaccgg tcgaagccct tgacctttca
    14941 gtgcgagtgt tcaattcgct caagcgaacc ggcatcacca ctgttggcga tgtgctggac
    15001 cttttggaaa aaggcgaaac agccgtcatg tccatccgca acttcggcga aaagagcctc
    15061 gatgaactac gccagaaaat gcgagaaaag ggctacatga aagacgaatc gagttctgcg
    15121 gagtaatttg caatgcgaca tcaagtatca ggttaccgac ttgggcgaac cacaggtgca
    15181 cgcctggcgc ttcgccgcaa tctaataaag cagtttttta cacacgagcg aattcagacc
    15241 acacgtgcca aggctgccgc gattcgcggt gaggccgaac gcctcattac aatcgcacga
    15301 aacagcgccg atggcagcga tatcgaaaaa gttaatgcgc gtcgcttggt gatctccaag
    15361 ctcggagata atcaggtcat caaacgtctt tttgatgaaa ttgcgccgcg ttttgcaaat
    15421 cgcaacggcg gttatactcg tgtgctgaag ctgggtcccc ggatgggtga ttcagctgag
    15481 atggttattt tggaattagt tgaggaataa gttctactta tcctgtagtt cgattttccc
    15541 ggcatcttgt gctgggtgac aatggcacgt taccaattga ctctcgtcta tgatggtacc
    15601 gactttttcg gaagccaacg gcaggcgaaa tccagaacgg tacaaggcga acttgaaaaa
    15661 gccttgaaca agttaggttg gatcggtcgc tcagtcatac tgtccgggcg gactgatacg
    15721 ggcgttcacg caacggggca ggtggcttcg tttgatcttg actggtctca cagcgaggct
    15781 aagctcatcg gtgcattgaa ctccgagttg cctcctgatc tgtccgttca gacggcgcgg
    15841 atcgtccacg ataaatttca tccgcgtttt gatgctacct cgcgctctta ccagtatcgg
    15901 ttgttttgtc agccaatacg tgacccgata cgagagcatt tcgcatggcg tgtttggcca
    15961 gcgatcaacc ccgatgctct cggccacttc gcaaagcttt ttcttggcac gcacgatttt
    16021 tctgccttcg gttcccggac tactcccaaa ggcggaacgg tgcgaaccgt agtgaaagct
    16081 gaatggacgc aaacaggcga agcgaaagac gaatggcgct ttgaagtaca ggctgacgcc
    16141 tttttatatc gaatggttcg gaagctggtg ttcgtccagg tggcggccgc acagggaaaa
    16201 gttttagctg agacaattac cagtgcttta gtcaagccag ctactgccaa aatcccagct
    16261 gggttagctc cagcacacgg gctgacctta gtccaggtaa catatgatga gtcaataggt
    16321 aaataagtaa agaaacggag atcagaaggt gtacaagtcg tactatccga aagcaagcga
    16381 aatcgatcgc aaatgggttt tggtggatgc caatggtcaa acccttggac gtattgccac
    16441 gcgcattgcc cacgtcctgc ttggtaagca caagccgagt ttcacgcctg gcgttgagat
    16501 gggtgatttt gtggtggtga tcaatgcgga gcgcgtaacg gtaacgggca cacgcaccag
    16561 cacgaagatg gaaacaaaaa tgtatcaccg acattccaat tatcccggtg gctataaggc
    16621 gatctcgtta cgcgaccaat tgcagcaaca tcccgatcgc gtgctgcgtg ccgcagtgtg
    16681 gggcatgctc ccacacaacc gcatgggacg cgctttgctc aaacgtttga aaatttacgc
    16741 tggcccaagc catccgcatg gcaccaacaa ccctgagccg ctggccactc cgcaagagtg
    16801 acgaaagagg aagagaatat gtctgttcag tattatgaag cagtaggtcg tcgcaaggaa
    16861 agcacagcac gcgtccgcgt gatgggtggt tctggccgat tcatcgtcaa tgataaggaa
    16921 gccaccgtgt atttctctcg gctcggcgat ctgcacgata tcctgcgcgc gttcacggct
    16981 gtgggccagg attcgaagaa gtatgatatt accgtgaagg tcaacggtgg tggcgttacc
    17041 ggtcagacgg aggcggttcg cctgggtgtc gcacgcgcac tggtgctcct caacggtgaa
    17101 tggatttccg cattgcgcaa gaatggcttg ttgactcgtg atgctcgcat caaagagcgc
    17161 aagaagccag gcctcaagaa ggcccgtaag gctccaacat acactaaacg ctagaaacat
    17221 tattttaaac ggatcaacgt aatgtgtaat cgcggtatga ttacgcatta cgttttgttt
    17281 ttaactggag tcctttatgg atttgtcact gatcgcgaaa gttttcgatg cccttggttt
    17341 ttcaccgcct tcgcggttga ttcttcttga agccgagact ctttcctcgg cgcacgttcc
    17401 gtccttcccg ccggatatcc ctgctttgct aattggggtt gattcaccgg acatggctct
    17461 gcatctgaaa actgttttgc tcacaaccta tcccgctgag catattgttc atatttttca
    17521 aaacggaatg acaaaagaag aacgactaaa tgattttgaa ttttccgaaa attcttgtat
    17581 ttatgttcct cctctagccg aaggaacatc ttttgaatcc tttgccgaga tcatcgccca
    17641 tctacgcgcg ccgaatggat gcccttggga tcgtgaacaa actcatgaga cgttgcgaaa
    17701 acacctgctg gaagaatcct acgaaaccat cgctgcaatt gattcgggcg acttcaaatc
    17761 catgcgcgaa gaatttggag atttgttatt gcaggttgta ttgcaggcac agattgcaaa
    17821 cgaagctgga caattcaatg tcaatcaagt gattcaggga atctattcga aaatcatacg
    17881 ccgccatccc catgtattcg gtgacttaaa gttggaaggc gtggaaggtg tgcttgcgaa
    17941 ttgggaaaag ctaaaagagc aggaaagagg agagaaaaaa gatggaaagg ggctactgga
    18001 tggcgttcct ggtgcgttgc ccgctttaag ccaggcgcag gaatatcagg accgagccgc
    18061 ccgtgtgggt tttgactggc cagagattga aggtgtactt gataaggtga aggaagaaat
    18121 tg
//
LOCUS       DASTJP010000459         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_497430, whole genome shotgun sequence.
ACCESSION   DASTJP010000459 DASTJP010000000
VERSION     DASTJP010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497430"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1250
                     /locus_tag="VFQ23_13375"
     CDS             195..1250
                     /locus_tag="VFQ23_13375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9907636.1"
                     /translation="MSRKSIISNPEIEIAPKRPLTDEEKLILSAKASADAIYDWDMVA
                     GVTTWNHGLHSLFGYEGDSVKAHTWWEEHIHPDDQERVIKSITAASQARSDYWTDEYR
                     FRRADGTYAFVVDRGYFIFDDQKPLRQIGAVIDITSRVALAEAQAQSAMEERQRLARD
                     LHDSVSQTLYSLTLIAEATRRLAQNGQLDQVIAQTERLRELSQQSLKEMRLLIFELRL
                     PTLEREGLAKALQQRLDAVEKRAGVDAKLIVENVDRLPVLVEEGLFYMAYEALNNSLK
                     HAAATKMTVSLRAIEDIVELQVVDNGKGFDVQSVSETGGMGLTNLNHRAQKLMAQLEI
                     TSIPEKGTSVKIVVHLT"
     gene            1267..1914
                     /locus_tag="VFQ23_13380"
     CDS             1267..1914
                     /locus_tag="VFQ23_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9907637.1"
                     /translation="MTEMIRIFIADDHAIVREGLRALLATEPGLELVGEASNGLQAVN
                     KVHDIRPDVILLDMVMPQMDGLEAILEIRKNWPEAKIIVLTSFSDDEKVFSAIRSGAL
                     GYLLKESSPQDLLQAIHSVARGEGFLSPTIASKVMRELNQPPKLPPTKDPLTEREVEI
                     LQLVAQGLTNDEIAEKLVVSERTVRTHVSNILAKLQLANRTQAALYALKEGLASL"
     gene            2087..2491
                     /locus_tag="VFQ23_13385"
     CDS             2087..2491
                     /locus_tag="VFQ23_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907638.1"
                     /translation="MSDQITIVIIATRSLPLADGLDALLKAIPQIEKVEIIRTIEQAM
                     QRVEEAKPRALLLDLALAGKKPEAFLEKIILHSPETLRVLLVDDVQDMKWQPQFAEAI
                     LIKGASPSAFATTLTNLLALKRDESEQIQSQE"
     gene            2565..>3116
                     /locus_tag="VFQ23_13390"
     CDS             2565..>3116
                     /locus_tag="VFQ23_13390"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9907639.1"
                     /translation="METKANTYRTTAKVVGALYILGFVVGIAGSALGAPGQLDSVSTR
                     GMMIAIGALLWVITAAGDAAHGVMMFPILKQNSERIALGYFGARIIEAAVIAISALFI
                     LLQIPLGAEFLKASASETSSLQSLSVLFIQSQASAYQIGMVTLGIAGLMLCYGFYRAK
                     LVPQFFVIWGFLGYVSFLGGSMLE"
BASE COUNT          863 a          741 c          767 g          745 t
ORIGIN      
        1 cttcatttga gctaattata tcgctgcgat gactctgcgg tttgccttca agcgagaacc
       61 tgggaccgac aagccctatg aagaaagcaa caaaaatcca aacggagtct tcccgatttt
      121 gcggcactct tgttcgttta attgcttagc cgttgaaaat ggagtggaat ctatccggag
      181 tataataatc ttcaatgtcg cgcaaatcaa tcatatccaa cccagaaatc gagattgcac
      241 caaaacgacc ccttaccgat gaggaaaaat taattcttag tgcaaaggcg tctgcggatg
      301 ccatttacga ctgggatatg gtcgcaggcg taacgacatg gaatcacggt ttgcactcct
      361 tatttggcta tgaaggcgac agcgtcaaag cccatacctg gtgggaggaa catattcatc
      421 ccgatgacca ggaacgagtg atcaagagta ttaccgcggc cagccaggca cggtccgact
      481 actggacaga tgaataccgc ttccgccgcg ccgatggaac atatgccttt gtagttgatc
      541 gcggatattt tatttttgac gatcagaagc cgcttcgcca gatcggtgcg gttatcgata
      601 tcaccagccg cgttgcactg gctgaagcac aagcccaaag cgccatggag gaacggcaga
      661 ggctggcccg tgacctgcat gattctgtct cgcaaacttt atacagtctc acgctgatag
      721 cggaagccac acgccgtctt gcccaaaacg gacagttgga tcaagtgatt gcccagacag
      781 aaagattgag agaactttcc cagcaatcgc tcaaagaaat gcgtttgctc atatttgaat
      841 tgcgcctacc gaccctggag cgagagggat tagcaaaagc ccttcaacaa cgtctggatg
      901 cagtggaaaa acgtgccggc gtagatgcaa aactgatcgt tgaaaacgta gacaggttgc
      961 cggttctggt cgaagaggga cttttttata tggcatacga agcactaaac aattccctaa
     1021 aacatgctgc cgccacaaaa atgacagtat ctttgcgagc gattgaggat atagtagaac
     1081 tccaggtggt cgacaatggc aaagggttcg atgttcaatc agttagcgaa accggtggaa
     1141 tgggattaac aaatctaaat caccgcgccc aaaaacttat ggcacaactg gagattacat
     1201 ctattccgga aaaagggaca agtgtcaaaa tagtggtgca tctcacctga attaggaaaa
     1261 cagaaaatga ctgaaatgat ccgaatcttt atcgccgatg accatgccat cgtccgtgag
     1321 ggcctgcgtg cgctgctcgc aacagaaccg ggactcgagc tggtcggcga agcgagcaat
     1381 ggtttacagg ccgtcaacaa agttcatgat attcggccag acgtgatcct gctggacatg
     1441 gtcatgccgc agatggatgg gctggaagca atactcgaga tcaggaagaa ttggccggaa
     1501 gctaaaatta ttgttttaac ttcgttttct gacgacgaaa aagtcttttc agcgatccgc
     1561 agtggcgctt tgggatacct gcttaaggaa tcctcccctc aggatttatt gcaggctatt
     1621 catagcgtgg cgcgcggtga aggcttcctc tcaccgacga ttgcatccaa ggtgatgcgc
     1681 gagcttaacc agccgccaaa attgccaccc acaaaggacc ctttgacaga aagagaagtg
     1741 gagatcctgc aacttgtggc gcagggcctg accaatgatg aaattgctga aaaactcgtg
     1801 gtcagtgaac gaacggttcg tacgcatgta agtaatattc tcgcaaaact acaactggca
     1861 aatcgcaccc aggccgcgtt gtatgcgttg aaagagggac tggcttcctt ataacgccat
     1921 aagtaattca tcattcaaaa caaaaaccac ttcggtggtt tttttgtttt ccagggcatc
     1981 cgtcgtcgaa ccgaatacgt catttgtcgt atttcaaatc taactatccg ccgatgcgat
     2041 aagagatcca aaacgataaa ctattgaaca taggaaaaaa caaagaatga gtgatcaaat
     2101 cacaatcgtc atcattgcaa cccgctccct tcccctggcg gatggactgg atgccttact
     2161 gaaagccatt ccacagatcg agaaggtgga gatcatccga acaatcgaac aggcgatgca
     2221 acgagttgaa gaagcaaaac cgagggccct cttattggat ttggcactgg caggcaaaaa
     2281 gcccgaggca tttctggaga aaattatttt acattctccc gaaacattgc gggtactgtt
     2341 ggtagacgac gtgcaagata tgaaatggca gcctcagttc gccgaagcca tcctgattaa
     2401 gggagcctca ccctctgcgt tcgccacgac cttgaccaat ttattagcct tgaagcgaga
     2461 tgaaagcgaa caaatccaat cccaagaatg acttttaaaa acgacaaacg attttgaact
     2521 tgctatcgat caatcaacat tattcaatca aaggagaaaa tatcatggaa acaaaagcta
     2581 acacatacag aacgacggca aaagtggtgg gcgcgctata tattctggga ttcgttgtgg
     2641 gcattgcggg atcggcactt ggtgcacctg gtcagcttga ctcggtttct accagaggca
     2701 tgatgatcgc aattggtgcg ttgctttggg tcatcactgc cgctggcgac gccgctcatg
     2761 gggtgatgat gttcccgatt ttgaagcaga atagcgagcg catcgcgctc ggctacttcg
     2821 gtgctcgaat tatcgaagcc gcggtcattg ctatctcggc gttattcatc ctccttcaaa
     2881 tcccacttgg cgccgagttc ctgaaagcca gcgcttccga aacttcttct cttcaatctc
     2941 taagcgttct gttcatacag tcacaggctt ctgcctacca gatcggcatg gtcacgttgg
     3001 ggatagctgg tctgatgctt tgctacggtt tctatcgggc aaaattggtg ccgcagtttt
     3061 tcgtcatctg gggcttcctc gggtacgtca gctttttggg tggatcgatg ttggaa
//
LOCUS       DASTJP010000460         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1374742, whole genome shotgun sequence.
ACCESSION   DASTJP010000460 DASTJP010000000
VERSION     DASTJP010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374742"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFQ23_13395"
     CDS             <1..470
                     /locus_tag="VFQ23_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9907640.1"
                     /translation="REFSLVNDLKVIAYCLMPTHYHILVRQDGDKPAGNLPQSVFNSY
                     SKAYNNMYSRSGTLFEGRFRAKPVQTSAHLLQLCRYIHGNPVKDGLVRDPADWKYSNY
                     LNWIDEKHTSLVDRDFIESQFDGVEGYKKFLFQYLKTRDLPDDVKMFLADLER"
     gene            complement(544..2001)
                     /locus_tag="VFQ23_13400"
     CDS             complement(544..2001)
                     /locus_tag="VFQ23_13400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9907641.1"
                     /translation="MSEADSPRFPIRALLFWGVGLIMALGLFTVVRDLTACWRLTSLP
                     GIPPASCAGPQVDAVEGPVIVNTKTAPTSTPEASVPEEITYPTWDGASRINILFVGLR
                     GGDPLEGDCPFCTDTLMLLTVDPLSKTAGMLSIPRDMWVNIPGFGFSRINTAWTLGRG
                     SKLPGGGPALAMKTVSHFIGVPVDYYVQVDFDTFVDVIDVLGGVDIQNDETLFLDPVY
                     HDREYPKVKLTCCGMRHLNGRVALAYARCRHAEQGCDDGDIGRARRQQKVIFAIRDKV
                     LNPENFPRLLSQSTKLYGTFYRGIQTNMTLQDAVKLAALMQDIPRGSIRNEVIDNKMI
                     GFGNVTLGGVNASIIRPLPDQIRILRDQLFTTRGALRPLATGDVITLMLADRARLRIL
                     NGTSTPDLDTRTANFFTSQGIVTIEVGKIPAINRTTIILYSPKLYTLRYLVDLFGITN
                     STPIRIRPDPAETVDIEIRLGPEWVKKLPAGELEK"
     gene            2611..2979
                     /locus_tag="VFQ23_13405"
     CDS             2611..2979
                     /locus_tag="VFQ23_13405"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9907642.1"
                     /translation="MGAILSFIIAVIVAAVVLMIVSRLNLGLSVANFTSAIIAALVIA
                     LVSLVVNWLLGALGIGIGGGFLGLIINLIVAALVLMISDRFVTGMTVGGFGGALVAAI
                     AIAVVNWLVGLLLAQLGLVA"
     gene            3278..>4053
                     /locus_tag="VFQ23_13410"
     CDS             3278..>4053
                     /locus_tag="VFQ23_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907643.1"
                     /translation="MNRTKQSFHPVFYVLMILYLIFAYSTLGWGESWAASLYPEDHYF
                     ENVGAVSFLAATVISLFVYMRAVTKRYITQMHWVKLSVYAVLALIYFFGAGEELSWGE
                     RIFLPPVLDSANAPEELKIQNLAIIENNEFLTGDNIYTLFWMGFAVAIPVLSRVWKRF
                     KGFAETFTPIVYWGIGVLFLLNYFFAQLANLFYRTAFNSQTILFPQALQEVKESNYQL
                     LFVFLSLLVLWDLNSRVFPKSNYGSPYRLPELNRVRTTDQP"
BASE COUNT          950 a         1003 c         1018 g         1082 t
ORIGIN      
        1 tcagggagtt tagcctggtc aacgatctca aagtaatcgc ctattgcctg atgcctactc
       61 attaccatat cctcgtacgg caggatggag ataagccagc aggtaatctg ccgcagtcgg
      121 ttttcaatag ctattcaaaa gcctataaca atatgtattc ccgcagcgga acgctctttg
      181 aaggacggtt ccgtgccaaa cctgttcaaa ctagcgcgca tcttttgcaa ttgtgccgtt
      241 atattcacgg aaatcccgtg aaagacggat tggtcaggga tcctgccgat tggaaatatt
      301 caaattatct gaattggatt gatgagaaac acacttcact cgttgatcgt gactttattg
      361 aaagtcaatt tgatggtgta gagggttata agaaattcct ctttcaatat ctgaaaaccc
      421 gcgatttgcc cgatgatgtg aagatgttct tagcggactt ggagaggtag aaagaagcga
      481 cacctcgaga tacataaagt gacagtcacc ttcaaggtga ctgtcacttt aaatctttac
      541 aagtcacttt tctaactccc ccgcaggcaa tttcttgacc cactccggac cgagccttat
      601 ctcgatatct accgtttccg cggggtcagg gcggatgcgg atcggcgtgc tgtttgtgat
      661 cccgaacagg tccaccagat atcgcaatgt atagagcttc ggtgaataga gaatgatagt
      721 ggtgcggttg atggctggta tcttgcctac ctcgatcgtg acgattccct gcgacgtaaa
      781 aaagtttgcc gtgcgcgtat caagatccgg tgtggatgtt ccgttcagaa tgcggaggcg
      841 tgcacggtca gccagcatca acgtgattac gtcaccggtt gcgagtgggc gcagcgcgcc
      901 tcgtgtggta aagagctggt cacgcagaat gcggatctga tcgggaaggg ggcggataat
      961 gctggcattc actccaccca gcgtaacatt accaaacccg atcattttat tatcaatgac
     1021 ttcgttacgg atgctccccc gaggaatatc ctgcatcaaa gctgctaatt tgacggcatc
     1081 ctgcagcgtc atgttggttt ggatacccct gtaaaaggtg ccatatagtt tggtcgactg
     1141 tgaaagtagc cgggggaagt tttccgggtt caataccttg tcgcggatgg caaagattac
     1201 tttctgttga cgcctggcac gcccgatgtc accgtcgtca cagccctgtt ctgcatgcct
     1261 acagcgcgca tacgccagcg caaccctgcc attgagatgc cgcattccac aacaggtgag
     1321 cttgactttg gggtattccc tgtcatggta aacagggtca aggaacagcg tttcatcatt
     1381 ttgaatatcc accccgccga gcacatcgat gacatccaca aaggtatcga aatccacctg
     1441 cacataataa tccaccggca ccccgatgaa gtgagagacg gttttcatcg ccagcgccgg
     1501 gccgcctccg gggagttttg aaccgcgtcc gagtgtccag gcggtgttga tgcggctaaa
     1561 gccgaagccc gggatgttga cccacatgtc gcgcgggatg gaaagcatcc cagccgtttt
     1621 tgagagggga tctaccgtca gcagcatcaa cgtgtcggta cagaacgggc aatccccttc
     1681 aagcggatcg ccaccgcgca gccccacaaa caggatgttg attcggctcg cgccatccca
     1741 tgtgggatag gtgatttctt ccggcacact cgcttcgggt gttgaggtgg gcgcggtttt
     1801 tgtattgaca atgaccggac cttccaccgc gtccacttgc ggacccgcac aactggcagg
     1861 cggaatacca ggcagggaag tgagccgcca gcatgcggtg aggtcacgaa cgaccgtgaa
     1921 aagacccaga gccataatca atcccactcc ccagaaaagc agggcacgga ttggaaagcg
     1981 tggggagtcg gcttcagaca ttacgcttgt aattgtatct tccttccggc ggtatgggag
     2041 tcactttgag actgcccagg aacgtgccga agcaggtatg gtgtgcgtag cgtgtcgtac
     2101 gccgaatcct gtactagtgc tgtactcgtc ctgtactgac cctgtactat gcttatgtat
     2161 tatccggaga cggttctata cctgtctttc aatatccatc caaattccaa atccccgtac
     2221 tcgtgtgccg ttttggaagg ataacttgca aagtgtagca tagcgtgtgg gcaagcagat
     2281 ttacagatgg ccaacaggaa ctgaatatag tatgtccctt acggcttccg ttgcaggcga
     2341 ttaaacttta caaggagaga ttcaacccag accgaaacgc gctcacttct tcttaattga
     2401 ttgccactcg agttctactt cttgtcggcg taacgaacgt ccaggtgagg cgttatataa
     2461 ctaaacacag cattacatta ttacattgtt gttggactgt ccgctacacg ctgtctgtat
     2521 ccggatattg ctgcccacca cacaatcgca atatctagat tcgaacctcg ctgtccattt
     2581 ccacacctac aaaaaaagga gatttgcaaa atgggtgcta ttttaagttt tatcatcgct
     2641 gtcatcgttg ccgccgttgt cctcatgatc gtgtccagat tgaacctggg attgagtgtc
     2701 gccaacttta cctcggcaat cattgcggca ttggtgattg ctttagtcag tctagtggtt
     2761 aactggctgc tcggtgcgtt gggcattggc attgggggag gcttcctcgg cctaattatc
     2821 aatctgatcg tggctgccct cgtgctgatg atcagcgata gatttgttac cggaatgacg
     2881 gtcggcggct ttggcggagc cctcgtcgct gccatcgcaa tcgccgttgt gaactggctg
     2941 gtgggcttgc tgctcgccca gttaggcctt gtcgcctagt ttactctcac agaaagcctc
     3001 aggcttacta ttgttcctgt ttatcctctc cggagaaaag acaggagcta aaaaaaaggc
     3061 aatccgcccg actcgagtcg ggcggattgc atcaggggaa gaatttgctc ctcctgttat
     3121 gttccgctgt aaatcattct tgtgaagttc gcggcttaac cttcctcact acgttgtgca
     3181 acttttgcga aatatcagcg tcatgtgcta gactgctcga ttggcaaact tgccggcgtt
     3241 tttccagtca gtcgaggtcc aagaggagat aggagcgatg aacagaacga aacaatcttt
     3301 ccatccagtt ttttacgtcc ttatgatttt gtatcttatt ttcgcctata gcaccctggg
     3361 gtggggtgaa agttgggctg ccagcctgta cccggaagac cactactttg aaaatgtagg
     3421 tgcggtttcg ttcctggctg cgactgtcat ctcactcttt gtgtatatgc gagccgtgac
     3481 caagcgttac atcacccaaa tgcactgggt caagttatct gtttacgcgg ttctggcgtt
     3541 gatctacttt ttcggcgcgg gagaggaact cagttgggga gaaaggatat tcttaccgcc
     3601 cgttttagat tcggcaaatg ccccggaaga attgaagatt cagaatttgg caatcattga
     3661 aaataatgag ttcctgaccg gagataacat ctacaccctg ttttggatgg ggtttgccgt
     3721 agccattccc gtactcagcc gcgtttggaa gcgctttaaa ggattcgctg aaacgttcac
     3781 gccgattgtt tattggggaa ttggagtact tttcctgctc aattatttct ttgcgcaact
     3841 cgccaatcta ttttatcgaa ctgcattcaa ctctcaaacg atcctatttc ctcaggcact
     3901 tcaggaagtc aaagaaagca attatcagct tttgtttgtg tttctttccc tgcttgtgct
     3961 ttgggatcta aattcgcgtg tatttccaaa atcaaactat ggctccccat atcgcttgcc
     4021 ggagttgaat cgagtccgaa ctacggatca acc
//
LOCUS       DASTJP010000461         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1259795, whole genome shotgun sequence.
ACCESSION   DASTJP010000461 DASTJP010000000
VERSION     DASTJP010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259795"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFQ23_13415"
     CDS             <1..582
                     /locus_tag="VFQ23_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907644.1"
                     /translation="SYEIPFPDLQFQILLGTQQVQEIDGQIAIVFTTETGQTFVEFQA
                     TTDQLNLSIIGREGDLIHNEVLLVPDETFGSYPVARVFSAGMAISPKDGQPLELPLTA
                     NRIPEFDAAGIPEEPNTLRPSLIIESVELVYFVNNPYYQVSDPNYERRSTYIQPVWHF
                     QGHYENGDAVDVLVQALRREFLSPEISPGITPG"
     gene            658..1416
                     /locus_tag="VFQ23_13420"
     CDS             658..1416
                     /locus_tag="VFQ23_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET9907645.1"
                     /translation="MITTYHRPKTLDETLTLLAQPNVLPLGGGTLLASSKPTSAPVEV
                     VDLQSLGLDAIKQSGNNLELGATLTLQALLESEHCPDALKSALKLEAPLNIRNAATVA
                     GTLVSGDGRSTLATILLALDAKIHATSKQKPEETINIGDFLPLREQIRGKLITKIIMP
                     LNVKLAFEYVARTPADKPIVCAAVAKWNSGRTRLALGGYGASPMLGMDGTESEGLETA
                     ARNAFHEATDDWASAEYRMDVAATLTKRCWDNIR"
     gene            1430..>2752
                     /locus_tag="VFQ23_13425"
     CDS             1430..>2752
                     /locus_tag="VFQ23_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907646.1"
                     /translation="MTKEQLDKKANQLGYLLVAIPLIIYWIYWQLNTSYWFNADPAAV
                     YFLDSLSVFVGRSYTYVDHPGTPIQLIGTLFLALTYPFLGRQEAFIDFHLARPGAFFL
                     MTNLFLLGMNIVTGFVFYKTVITTLNVDRLLGGVSIALAYFALHPYSFPSLTLWSHNS
                     LNFPFGTLWLIWLFVELRKEQEIPRFKLILLGVAAGILLMAQMYFAAWLVSGIFTIFI
                     FSFRLDRSISKAITSGSYLLAGGIIGIIAMLIPIYKELPRFFSWLTRIITHQGLYGSG
                     EAGIYSLAFIPIAINFWWANIRPMILVLLATLIVVTLIAYAKNKSTEKVSPSMFAMVS
                     GLVFQILLILLLMTKAALKLRYSLSLAAILPLLILLTVKLLETMPWRTILFRRVFYVL
                     LLTGVGFNLVNQVVEQRQRAFVEADAALAKSQAVTHLAREKNVLEDDIV"
BASE COUNT          670 a          668 c          653 g          761 t
ORIGIN      
        1 agttacgaga tcccattccc ggatttgcag ttccagattc tgttgggaac ccagcaggta
       61 caggaaatcg atggacaaat tgccatagta tttacgacgg aaaccggaca aactttcgta
      121 gagttccagg cgactacgga tcaactaaat ctgtcgatca ttgggcgcga aggtgacctc
      181 atccataatg aagtgctgct cgtcccggat gagacttttg gttcgtaccc ggtcgctcga
      241 gttttcagcg ctggaatggc gattagcccg aaagatggcc agccgttgga attgccactg
      301 actgcgaatc gaattccaga atttgatgct gcggggattc ctgaagagcc caatacgctt
      361 cgacccagct tgatcattga atcggttgaa cttgtatact ttgtcaataa tccgtattat
      421 caagtgagtg atcccaatta cgagcggcgc tccacctata ttcaacccgt ttggcatttt
      481 caaggacatt acgagaatgg agatgcggtt gacgtgctgg ttcaggcatt aagacgggag
      541 ttcctctcac cagaaatctc acccggtatc acacccggat gatcaaatcc ctaccgccat
      601 cacaacgatg gcggtaactt tttaacttat aataaagcta tccctcggag ataacttatg
      661 atcaccactt atcatcgccc caagacgctt gacgaaacgc ttacgcttct tgcccagccg
      721 aacgtattgc cgcttggagg tggaacgctt ctcgcttcaa gcaagccaac ttctgctccc
      781 gttgaagtgg tcgatcttca atcacttggg ttggatgcga tcaagcaaag cggaaacaat
      841 cttgaacttg gcgcgactct tacacttcaa gcgttacttg aaagtgagca ttgtcctgat
      901 gccttgaaat cggccctgaa acttgaggcg ccgctaaaca ttcgcaacgc tgccacagtt
      961 gccgggactc tcgtttccgg cgatgggcga tcaacgcttg caacaattct gcttgcactg
     1021 gacgccaaga tccatgcaac tagcaagcaa aaaccagaag aaactattaa tattggagat
     1081 tttcttccgc tgcgagagca gatccgcggc aagttgatca cgaagatcat tatgccgttg
     1141 aacgtcaagc ttgccttcga atatgttgct cgtaccccag cagacaagcc gattgtttgc
     1201 gctgcagtgg ccaaatggaa ctcaggtcgc acgaggctgg cgttgggcgg atatggagca
     1261 agtcccatgc tgggaatgga tggaaccgag tctgaagggt tggaaaccgc cgcgcgcaat
     1321 gccttccatg aagcgaccga tgattgggcc agcgcggaat atcgaatgga tgtcgctgcc
     1381 acattgacaa agagatgttg ggacaacata cggtaggtca cgccttcgca tgacgaaaga
     1441 acagttggat aagaaggcaa atcagcttgg ctacctcttg gtagccatcc ccttaatcat
     1501 ttactggatt tactggcaac tgaatacaag ctactggttc aatgcagatc cggctgccgt
     1561 ttatttttta gactcgctgt ctgtatttgt cggcagatcc tatacatatg tcgatcatcc
     1621 cggtacgccg atccaactca ttggcacatt attcctggcg ctgacctatc catttctagg
     1681 ccgtcaggaa gcctttatcg attttcacct tgccagaccg ggcgcatttt ttctcatgac
     1741 aaatttgttc ttgttgggaa tgaatattgt cacagggttt gttttctaca aaactgtgat
     1801 caccaccctg aatgtagatc gattattggg tggcgtgtcg atcgctttgg cctattttgc
     1861 gctccatccc tacagctttc catcgctgac gttatggtcc cataactctc tcaattttcc
     1921 atttggaact ctttggctga tctggttgtt cgtggaactg aggaaggaac aggaaattcc
     1981 acgtttcaaa ctaattcttt tgggggttgc agcaggcatc ttattaatgg cgcaaatgta
     2041 tttcgccgcc tggctggtga gcggtatttt taccatattt atcttttcat ttcggctgga
     2101 ccgatccata agtaaagcca ttacttctgg ctcgtatttg ctggctggcg gcatcatagg
     2161 gattatcgcg atgctgatcc ctatctacaa agagctcccg cgctttttct catggctcac
     2221 tcgtatcatc acgcatcagg gtctttatgg aagcggtgaa gccgggattt attcacttgc
     2281 attcattccg attgcgatca acttctggtg ggccaatatt cgtcccatga ttttggttct
     2341 ccttgccacc ctgatcgtgg tgaccctaat tgcttatgcg aagaataaat ctacagaaaa
     2401 agttagcccc agtatgttcg caatggtctc cgggttggtg tttcaaattt tattaatcct
     2461 gctcttgatg accaaagccg cgctgaagtt gagatattcg ttatccctgg cagctatact
     2521 tcccctgctg attttgctta ccgtgaaatt actcgaaacg atgccgtggc gaactatttt
     2581 attcaggcga gtattttatg tgctcctgct cactggtgtg ggcttcaatc tagttaacca
     2641 ggtagttgaa caaagacaga gagcatttgt cgaagcagat gcagcccttg ccaaatccca
     2701 ggctgtgacg cacctggcgc gagaaaagaa tgttttagag gatgacattg ta
//
LOCUS       DASTJP010000462         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1069421, whole genome shotgun sequence.
ACCESSION   DASTJP010000462 DASTJP010000000
VERSION     DASTJP010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069421"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1519
                     /locus_tag="VFQ23_13430"
     CDS             131..1519
                     /locus_tag="VFQ23_13430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9907647.1"
                     /translation="MNSSRLTGMPAFAIIWSGQVLSLIGTALFSFAIGIWLYQQTGLA
                     TTFTTMIFFSNIPRIVLSPFAGVLVDRWNRKLTMMVSDLASGMTTLIILFLMWSGSLE
                     IWHLYVLLALSSAFESFQFPAFSSSITLLVEKKHYARTSAMMSLAEEGSRVLAPLLAA
                     ALIVVIKLEGIILIDVISFIVAIGTLLLVPIPQPPQTETGEKARNGILKDSVYGFRYF
                     LANPSLLGLQFNFLMVNLLAMAAALRTPMILARTGNNETILGTVASISAIGGVAGGLV
                     MSIWGGPKRRILGVLGGLILVNVGRAAMGLGHEVVVWSISGFFAVFFIAVCNSSNQAI
                     WQAKTPPDVQGRVFAARRFTGQLSLPLGALIAGPLSDRLFEPAMAVKGSLTPIFGGLV
                     GTGPGAGMSLLIFLAAIIGILVPALSYAIPVFRNVEDIIPDNDIASSHLSKSADIVRN
                     EPKERIPRPIAK"
     gene            1516..1947
                     /locus_tag="VFQ23_13435"
     CDS             1516..1947
                     /locus_tag="VFQ23_13435"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HET9907648.1"
                     /translation="MTNWLLLHYKLPNKPSALRVYIWRKLKRLGAILLHEAVWIIPDQ
                     PRTAEQVQWLTAEIQEMGGEAYSWKATAILGESDESITQQFKEQVDAVYSDLLKKLAK
                     KRVELQEVSRAYQQAAAQDFFHSELGLRVREKLTSKRGEKL"
     gene            1944..>2353
                     /locus_tag="VFQ23_13440"
     CDS             1944..>2353
                     /locus_tag="VFQ23_13440"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HET9907649.1"
                     /translation="MKWVTWENIGVDRMASAWLIRRWIDPEAEFSFIPMGEKPLPENG
                     EPFDIPGERFSHHGGHCTFYALLKENKIKNPILARIAQIVDEADEVQEVMVEPAASGL
                     DLICRGLKQISKDDFEAMERGGLIYEALYAQLEL"
BASE COUNT          585 a          566 c          609 g          593 t
ORIGIN      
        1 aataagcgca gtaacgtttc gaataccagc tttataatag ggtcagggac tacataagcg
       61 aacatatttg gtttgccctc ttccaaaaaa acaagtataa tgtaaccact gttacatttg
      121 gagacatgga atgaattcct cgcgtttgac cggcatgcct gccttcgcca tcatttggtc
      181 tggtcaagtt ctctcattaa ttgggacagc tttattttcc tttgcgatcg ggatctggct
      241 atatcagcaa accgggctgg caaccacatt cacgaccatg atcttctttt cgaacatacc
      301 cagaatcgtg ctcagcccat tcgcgggcgt attggtggac cgctggaatc gtaaactgac
      361 aatgatggtc agcgatctcg cgagcggcat gacgaccctc ataattcttt tcctgatgtg
      421 gagtggctcg ctggaaatct ggcatttata tgtgttgctg gctttgtcga gcgcgtttga
      481 gtcgtttcag tttccagcct tttcgtcatc gattactttg ttggtcgaaa agaaacacta
      541 cgcacggacc agcgcgatga tgtcgctggc ggaggaaggc tcccgtgtgc tggcgcctct
      601 actggcggcg gcattaattg tcgtgatcaa attggaaggg atcatcctaa ttgatgtcat
      661 ctcctttatc gtggcaattg gaaccctact tcttgtgcca atcccgcaac cgccccagac
      721 ggaaacgggg gaaaaagcca ggaacgggat cttgaaggac tctgtatatg gctttcgtta
      781 ttttcttgca aacccgagcc tgctgggtct gcaatttaac ttcctaatgg tcaatctact
      841 ggcaatggct gctgcgttgc gcacaccaat gattctggcc cggacaggaa acaatgaaac
      901 catccttggg acagtcgcat cgatttcagc aataggtggt gtggcaggcg ggctggtgat
      961 gagtatctgg ggcgggccta aacgaagaat actcggggta ttgggcggct taatcctcgt
     1021 caatgtggga cgcgctgcga tgggactggg acacgaagtc gtagtatggt caatttcagg
     1081 attcttcgct gtatttttta tcgcggtgtg caattcatcc aaccaggcga tatggcaggc
     1141 aaagacgcct ccagacgtac aggggcgcgt ctttgcggcg cgacgattta cgggccagct
     1201 ctcgctccca ttaggcgctt tgatcgcagg acccttgagt gatcggttgt ttgagcccgc
     1261 aatggctgtg aaggggagcc tcacccccat tttcggagga ctggtgggca ccggccccgg
     1321 cgcaggtatg tcgcttctga tattccttgc cgcaataatc ggtattcttg ttccagcact
     1381 aagctatgct atacccgtct ttagaaatgt cgaagacatt atccccgata atgatattgc
     1441 ttcgtctcat ctatcaaaat ctgcagacat cgtccgcaat gaaccaaaag agaggatacc
     1501 gagaccaata gcaaaatgac caactggctc ctccttcact acaaactacc caataaaccc
     1561 tcagccctcc gcgtttatat ctggcgaaag ctaaaacgcc ttggtgcaat ccttttgcac
     1621 gaggcggtct ggatcatccc cgaccagccg cgcacggcgg agcaggtcca gtggctaacc
     1681 gcagaaatcc aggaaatggg cggcgaggct tattcgtgga aagccactgc gatcttaggg
     1741 gaaagcgatg aatcgattac acagcaattc aaggagcagg ttgatgcagt ctattcggat
     1801 ctcttgaaaa aacttgctaa gaaaagagtc gagttgcagg aagtttcgcg ggcatatcag
     1861 caagccgcgg cgcaggattt cttccattca gagcttggat tgcgtgtacg cgaaaaatta
     1921 acctcaaaac gcggagagaa attatgaaat gggttacatg ggaaaatatc ggtgtagaca
     1981 ggatggcttc tgcatggctc atccgccgct ggattgaccc ggaggctgaa ttctctttca
     2041 ttcctatggg cgaaaaacca ctgcctgaaa atggtgaacc gtttgatata ccaggtgaac
     2101 gtttttcgca tcatggcggt cattgtacat tttatgctct tctaaaagaa aacaaaatca
     2161 agaatcccat cctcgcccga attgcacaga tagtggatga agctgacgaa gtccaggaag
     2221 tcatggtcga acccgctgcc tcagggctgg atctgatctg ccgcgggctg aaacaaatca
     2281 gtaaggatga tttcgaggcg atggaacgtg gcggtttgat ctatgaagct ttgtacgccc
     2341 aactagaact gga
//
LOCUS       DASTJP010000463         8284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_842527, whole genome shotgun sequence.
ACCESSION   DASTJP010000463 DASTJP010000000
VERSION     DASTJP010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842527"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="VFQ23_13445"
     CDS             <1..1599
                     /locus_tag="VFQ23_13445"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9907650.1"
                     /translation="SPHKYSRIFVYSFSLLALVISSCAGAGPSNAYPTYDPFAPISGA
                     GTQIAPVQPGQVIIEAGTTQTGPTPALAPVSVTLAPTDVDPSFSSPTPDQAHVLPAPR
                     DVVDQYTVQPGDTLGSIAQGYGITLEELMQANGLNESSILSVGTLLNIPPISADLNPG
                     SSFKIIPDSELVYGPSSVGFDLDAFLENKGGYLTNYVQDLNGEFVSGSEIIMRVAQNY
                     SVNPRLLVALIEYRSGWVTNPVPQNIDYPLGNFDNYYAGLYRQMTWTADNLNRGYYLW
                     RVGGISTLPLSDGSYVPFDPTINAGTAGVQYFLSLYNSRTFWDQDVGILGLFQTYRDL
                     FGYPFDYSVVPLVPQNLTQPPFQLPFEVGVTWSFTGGPHGGWDSGSAWAALDFAPPGE
                     PAGCVISDAWVVASANGRIVRAEDGAVVQDLDNDGYEQTGWSILYMHIETRDRVRPDS
                     YVYAGEIIGHPSCEGGFSNGTHVHIARRYNGEWIPADGSLPFNLDGWISSGNGIEYDG
                     FLTRGETTVEAWEGVFEGLNQIAR"
     gene            1706..3334
                     /locus_tag="VFQ23_13450"
     CDS             1706..3334
                     /locus_tag="VFQ23_13450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9907651.1"
                     /translation="MKRAIIALIISSSLLASCVGNASLWGQYSTPTPLGGVPPTASPI
                     PQIFPTQTPVPIDPLSFGITTTNTPSPTLVNDAFVTQAVTSVVETPTFAVDGQSILYY
                     SQNGDWLPAVANRFGVAVSEITSPKILPQAGYLDTGTLLIIPDRSEDTMQLTQSLQII
                     PDSEFVFSATAVDFDISAYVRDAGGYLSTYREYLGTTGWTTGAGVIERAAYENSVNPR
                     LLLAILDFEAHWVRGNPENEFRTEYPMGYENFRNKGMFLQLAWAVNQMSMGYYGWRAG
                     SFTELTFRDGSILRLDPTLNAGTVAIMMLFSRMHSLNEWLRIMDQTSGFMSFYQNMFG
                     DPWVRAEASGPIFPPGLTQPEMALPFETHTPWVFTGGPHSAWGTHRPGEPNGALAALD
                     FAPASEKTGCDPSTTWIVSTAPGLVVRTGRGFVVVDMDGDGSEQTGWNILYLHVANQS
                     RVSEGQWVETNDLIGHASCEGGVSTGTHLHVARKYNGEWVVADGPISFVLSGWTVLAG
                     DKPYEGKLVKGNKIIVADVYGQSRALIFREDEGQ"
     gene            3342..4847
                     /locus_tag="VFQ23_13455"
     CDS             3342..4847
                     /locus_tag="VFQ23_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9907652.1"
                     /translation="MNSKLNSLRKHKRQEAIVCLSTAFLIVSLVISSCAPQVTHGNLE
                     SIITPLAPTSMPAPTSGRPEYPPGELVDYTAQNGDSLPALAARFNTTVAEIREANPII
                     PLDATTMPPGMPMKIPIYYLPLWGTAYQSIPDQAFVNGPAQVGFSTSAFVASTNGWLK
                     NYRIYAGEHMRTGAEVVDYVATNYSVSPRLLLALLEYQAGALTQPEIPSGKYLLGFRR
                     SFYEGPYLQLVLAANMLNNAYYNWRGGKLTEFELLDESLFRPDPWQNAASVSLQYYFS
                     RLYSGDKYFTSIGPDGFASVYRDLFGDPWADPITFIPGSLQQPSLRFPFLAGHIWAYT
                     SGPHTGWGTGEPLAALDFAPASDTSGCFTVARDLYTTAMADGLIVRATVDGVIVDLDK
                     DGDERTGWVLFYLHTATDSRIFVGKEVKAGDPIGYPSCEGGSSTGTHVHVARKYNGEW
                     ILAAGAVPFNLEGWVAGGGEVSREGTLRRGPLTVVACECSDFGTQVQSEIK"
     gene            complement(4876..5352)
                     /locus_tag="VFQ23_13460"
     CDS             complement(4876..5352)
                     /locus_tag="VFQ23_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9907653.1"
                     /translation="MKATVTWQSNMVFVGQPASGFPISMDSDASYGGTNSGIRPMEMI
                     ALGLAGCIAMDVLSILQKKRQQVTHFEVRLDAPRSPEYPMVFTSAAITYVVTGKNINE
                     EALLRSIDLTATKYCPAQIMLEKAFPMTLHYEIYEAGSDGNKELKFQGIWQKTALE"
     gene            complement(5377..5943)
                     /locus_tag="VFQ23_13465"
     CDS             complement(5377..5943)
                     /locus_tag="VFQ23_13465"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9907654.1"
                     /translation="MIESKTITQSMKSMPVVLVILGIVLVAVATFFILRDLPPQTSFR
                     TVPVKVNYPSPDLTLKDIQGISHTLADYRGQIVLINLWATWCIPCREEMPALQSFYNK
                     YKDSGFVVIAINDGDAEQEVLEFAQEYDLSFPIWLDPTYLATEQAFKTMNLPSSFVID
                     REGTVQLRWVGGISRQMLETHVTPLIQK"
     gene            complement(6057..6695)
                     /locus_tag="VFQ23_13470"
     CDS             complement(6057..6695)
                     /locus_tag="VFQ23_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907655.1"
                     /translation="MELRRFDPRLWLGVLLVFGGLLALLDAMGVISNAGGIFWGLIFA
                     AAGALFLYILINDRDNWWAAFPAFTLFGLAAQAFLPSSLEAFDGLVFFAGISLAFWWV
                     YFTDRSRWWAIIPAGVLLTLGATSVLDTLVAGDSDGGLFFLGLGLTFILVAILPGGKD
                     RQWAYFPGVALLIFGALLGTPISGFSEYFWPAALILLGGYFVVRFFMSRSSE"
     gene            complement(6743..7525)
                     /locus_tag="VFQ23_13475"
     CDS             complement(6743..7525)
                     /locus_tag="VFQ23_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907656.1"
                     /translation="MKRDNLFWGAALILFGFLLFLQQSGIIANVFTFFWPVTLILVGG
                     WLILSVYWRGDQSPDETFSIPLGTAKRVDYKFSHGAGQIEISGGTSASTALVGTTGMG
                     MNHKNRLNGESLEVHVEAGPSLVPFIGPSQGIWRFQLTKEVPVTLSVEAGASSIDINL
                     LDVQASRIELSTGASSSNVTLPARGMSFLEVESGAAAVNIRVPEATAARIHVEEGVSA
                     INVDTNRFPRLSSSLYQSSDFDTAIDRAEINIESGLGSVNVK"
     gene            complement(7512..8048)
                     /locus_tag="VFQ23_13480"
     CDS             complement(7512..8048)
                     /locus_tag="VFQ23_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907657.1"
                     /translation="MRQNRTNLVLGILLILIGGWLFATRQIPALQEWIDSNFAWPMWT
                     IGAGVLVLLIGLITGAPGMAVPASIIAGIGGILYYQNATNDFSSWSYMWTLIPGFVGV
                     GTILAGLLGESTRANLARGTSLLVTSAVLFLVFATFFGGLNILGEYGAPVILILLGLY
                     VLARGFLRSGSGGQNETR"
BASE COUNT         2013 a         2168 c         2070 g         2033 t
ORIGIN      
        1 tccccccaca agtactctcg aatatttgtt tattcttttt cgctacttgc tctggttatt
       61 tcatcctgcg cgggtgccgg accatccaac gcgtatccca cttacgatcc atttgcgcct
      121 atcagtggag cgggtacaca aatcgcgccg gtccaaccgg ggcaagtcat tattgaagcg
      181 ggaacaactc aaactggccc cacaccagca ctcgcaccgg tttctgtcac gcttgcgcca
      241 acagatgtgg acccttcctt ctcctcccca acacccgatc aggctcatgt actgcctgca
      301 ccgcgagacg ttgtcgatca atacaccgtc caaccgggag atacacttgg tagtatcgcc
      361 cagggatatg ggatcacgct tgaggagttg atgcaggcaa atggattgaa tgaatcgagc
      421 attctatcgg ttggcacgct gttaaatatc cctccgatca gcgcagacct caaccctggt
      481 tcttccttca aaataattcc tgattcagag ttggtctatg gaccttccag cgtgggtttt
      541 gatctggatg cattcctgga aaataaaggt ggttatctca ccaactacgt gcaggatctg
      601 aatggtgaat ttgtaagcgg cagtgagatt atcatgcgcg tcgctcagaa ctattccgtc
      661 aatccacgct tattggtggc gctcatcgaa tatcgaagtg gatgggtaac caaccccgtc
      721 ccgcaaaata ttgattaccc tcttggaaat ttcgataact attatgccgg gctatacagg
      781 caaatgacct ggacggcaga taacctgaac cgcggttatt atctctggcg cgtgggcggc
      841 atctccaccc ttccgttgag cgatggaagc tatgtccctt ttgacccaac tatcaacgcc
      901 gggacggccg gcgttcaata ttttctttcc ctatacaaca gccgcacgtt ttgggatcag
      961 gacgtgggca ttttgggatt gttccaaacg tatagggacc tcttcggcta tccatttgac
     1021 tacagtgtag ttcccttagt tcctcaaaat ttgacgcagc ctccatttca attgccattc
     1081 gaagtaggtg ttacctggtc ctttacgggt ggacctcatg gaggttggga ttctggctcc
     1141 gcctgggcag cattggattt tgctccgcct ggtgaacctg cggggtgtgt aattagcgac
     1201 gcctgggtgg tggcctccgc caatgggcgc atcgttcgcg ccgaggatgg ggctgtggtt
     1261 caggatctgg ataacgatgg atacgaacag accggctgga gcatccttta catgcacatt
     1321 gaaacgcgcg atcgagtgcg cccggatagc tatgtgtatg caggtgagat catcgggcat
     1381 ccatcctgtg aaggcggatt ttcaaacgga acccacgtgc acatcgcccg ccgctataac
     1441 ggcgagtgga ttcccgcaga tggcagtctg ccattcaacc tggatggctg gatttcaagc
     1501 ggaaatggca tcgaatacga tggcttcctt acgcgcggcg aaacaacggt cgaagcgtgg
     1561 gagggagtat ttgaaggact gaatcagatt gcgagatgat tctctcgtaa atttgaactt
     1621 ctcatctcca ctttattatc atttgttact ctcctgtata gagaacccct caatacatgt
     1681 ataattccgt gaacggattg aaatcgtgaa gcgcgccatt attgccctga ttatttcgtc
     1741 ttcattgctt gcgtcttgtg ttgggaatgc ctcgctgtgg ggacaatatt ccacaccgac
     1801 tccgcttggc ggcgttcctc caacagcctc acctattcct caaatattcc ccacgcaaac
     1861 gccagtcccg atcgaccctc tctcctttgg aatcacaacg acaaataccc cgtcgcctac
     1921 tctcgtaaat gacgcctttg tgactcaggc ggttacctcg gttgtcgaaa ctccaacctt
     1981 cgcagtggac ggacaatcca ttctatatta ttcacaaaat ggagattggc ttccagctgt
     2041 cgcgaatcga tttggtgtgg cggtgagtga gatcacctct ccgaaaatcc tcccgcaagc
     2101 agggtatctt gacacgggta cattgcttat tatccctgat cgtagcgaag atactatgca
     2161 gttaacgcag tcgctgcaaa tcattccgga cagtgagttt gtcttttcgg cgactgccgt
     2221 cgatttcgat atctcggcct atgttcgaga tgccggcggt tatctttcca cctatcgtga
     2281 gtatctcggc acgacaggct ggaccacggg agccggtgtg atcgaacgcg ctgcttacga
     2341 aaattcagtg aacccgcggc tcttgcttgc aatccttgat tttgaagcgc actgggtgcg
     2401 cggcaaccct gaaaacgagt ttcgtactga gtatccgatg ggatatgaaa acttccgcaa
     2461 taagggcatg ttcctgcaat tggcgtgggc tgtcaatcaa atgtccatgg gctattatgg
     2521 ctggcgggcc ggcagtttta ccgaactcac gttccgtgat ggaagcattt tgcggctgga
     2581 tcctactctc aatgcgggaa ccgttgctat catgatgttg ttttcacgca tgcacagcct
     2641 caatgaatgg ctgcgtatta tggatcagac cagtggcttc atgtcttttt accaaaatat
     2701 gttcggtgac ccctgggtac gcgctgaagc cagcggaccg atcttcccgc ctgggctcac
     2761 gcaacctgaa atggctctgc cttttgaaac gcataccccc tgggtcttta cgggcggacc
     2821 gcatagtgcc tggggaacac atcgccccgg ggaacctaac ggggccctgg cagccctgga
     2881 ctttgctccg gcaagtgaga aaaccggctg cgacccatca acaacctgga tcgtatccac
     2941 cgcgccaggg ttggtggtcc gtaccgggcg aggttttgtt gttgtggata tggatggtga
     3001 tggttccgag caaactgggt ggaatatttt atatttgcat gttgccaatc aaagccgtgt
     3061 gtccgaaggt cagtgggtgg aaacgaatga tctcattgga catgcctcct gtgaaggcgg
     3121 cgtctcaacc ggcacacact tgcatgtcgc gcgaaaatac aacggggaat gggtcgttgc
     3181 agatggtcct atttcctttg tgctcagcgg ttggacagtt ctcgctggcg ataagcctta
     3241 tgaaggaaag cttgtcaaag gcaataagat tatcgttgct gatgtatatg gtcaatcacg
     3301 ggcattgata ttccgtgaag atgaaggtca ataattcccg aatgaactca aaacttaata
     3361 gtttgcgaaa gcacaagagg caagaagcca tcgtgtgtct ttcgacagct tttttgatcg
     3421 tttctcttgt catctcatcg tgtgcgcccc aggtcacaca cggtaatctt gagagtatca
     3481 tcactccttt agcgcccacc agcatgcctg ctccaacctc cggccgaccg gagtacccac
     3541 cgggcgaact tgttgattac accgcgcaaa atggtgattc gctcccggct ctcgcggcgc
     3601 gtttcaatac gaccgtggcg gagatccggg aggcaaaccc gatcatccca ctcgacgcga
     3661 caacgatgcc gccgggcatg ccgatgaaga ttcccattta ttaccttcct ctctggggga
     3721 ctgcgtatca aagtattcca gaccaggcct tcgtcaacgg tccggcacaa gtgggtttca
     3781 gcacatcggc gtttgtagct tcgacgaatg gatggttgaa gaattaccgc atctacgccg
     3841 gagaacatat gcgtaccggg gcagaggtgg tggattatgt tgccaccaat tacagcgtca
     3901 gccctcggct cttgctggcg cttcttgaat atcaggcggg cgcactcacc cagcccgaaa
     3961 tcccctccgg aaaatacctc ctgggtttta ggcgatcttt ttacgaaggt ccatatttgc
     4021 aacttgttct cgcagcaaat atgctcaata acgcctacta caattggcgc ggagggaaac
     4081 tcaccgaatt tgaattgttg gatgaatctc tattccggcc agatccgtgg cagaacgcgg
     4141 cttccgtttc gcttcaatat tatttttcga gattgtattc tggagataaa tacttcacat
     4201 ccatcggacc ggatggtttc gcgagcgtgt atcgggattt attcggcgac ccgtgggctg
     4261 accccattac ctttattccc ggtagcctac aacagccatc cttgcgattc ccgtttttgg
     4321 caggtcatat ctgggcatac accagcggac ctcacaccgg ctggggaaca ggtgagcctt
     4381 tggctgccct ggactttgcg cctgcatcgg atacctcggg ttgtttcact gtggcgcgtg
     4441 atctctacac aactgctatg gcagacggct tgatcgtacg cgcaactgtg gacggtgtga
     4501 tcgttgatct ggataaggat ggagatgaac gaacgggctg ggttcttttc tacctgcata
     4561 ctgcaaccga ttcgcgcatc ttcgtcggaa aagaagtcaa agctggagat ccgatcgggt
     4621 atccctcctg cgagggcggt tcctcgacag gcacgcatgt gcacgtggca agaaaatata
     4681 atggagagtg gatcctggct gctggcgcag tcccctttaa tctggaggga tgggttgcag
     4741 gtggcggaga agtgtcgcgc gaaggaacgc ttcggcgcgg accgttaacc gtggtggcgt
     4801 gtgagtgttc tgattttgga actcaggtgc aatcggagat aaagtagact acaagtaaga
     4861 agctctgatc gttgtttatt ccaacgcggt tttttgccag ataccctgaa actttaattc
     4921 tttgtttcca tccgatccag cttcatagat ttcataatgt aacgtcatag ggaatgcctt
     4981 ctcgagcatg atctgtgccg ggcagtattt tgttgctgtc agatcgatgg atcgaagaag
     5041 cgcctcttca ttgatatttt tccccgtcac aacgtaagta attgcagcac tggtaaagac
     5101 catcggatac tccggcgagc gcggcgcatc cagtctcact tcaaaatgag taacctgctg
     5161 acgttttttc tgcaatatgg aaagcacatc catcgcgatg caaccagcca gacccagcgc
     5221 gatcatttcc atcggccgaa tcccgctatt tgtgccgcca tacgatgcgt ctgagtccat
     5281 tgatatggga aagccagatg caggttgacc aacaaaaacc atattgcttt gccaggtgac
     5341 agttgctttc atggtttctc catgttttaa aagattctat ttctggataa gtggcgttac
     5401 atgtgtttcc agcatttgac ggctgattcc cccaacccac cgtagttgaa ctgttccctc
     5461 tctatcgata acaaatgaac tgggtagatt cattgttttg aaggcctgtt cggtcgcgag
     5521 atacgttggg tcaagccaga tcgggaaact caaatcgtac tcttgcgcaa actctaatac
     5581 ctcctgctca gcatcaccat cgttaatagc aatgaccaca aatccgctat ccttgtactt
     5641 gttgtaaaac gattgcagag ctggcatctc ttctctgcat ggaatacacc aggtcgccca
     5701 taaattgatg agcacaatct gcccccgata atcggcaagg gtatgcgaga tgccttgtat
     5761 gtcttttaac gtcaggtcag gcgatggata atttaccttt accggaactg ttctaaaact
     5821 ggtctgtggc ggaagatccc ggagaatgaa gaaggtggcg acggcaacca gaacgatgcc
     5881 caggatcaca agaacaacgg gcatactttt catcgattgt gtaatcgttt tggattcaat
     5941 catgattgca ataagtatac ctcttcgaat aattcattca ttacgcaaaa aaacggggcg
     6001 cctgccagca tcccgttttt tttgaacacc gattcgaaat cttttgtagc ccgcagctat
     6061 tctgatgacc tgctcataaa aaaccgaacg acgaaatatc cgcctaacag gatgagcgcg
     6121 gccggccaaa aatattctga gaatccgctg atcggtgtgc ccagcagcgc tccaaagatg
     6181 agcagtgcaa cgccggggaa gtacgcccac tggcgatctt ttccgccggg caaaatagcg
     6241 accaggataa atgtgagccc cagccccagg aagaataatc caccatcact gtcaccagca
     6301 acgagcgtat cgagtaccga ggtcgcgcca agcgtaagca aaacgccagc cgggatgatg
     6361 gcccaccaac gtgatcggtc ggtgaaatac acccaccaaa aggcaaggct gatgccagcg
     6421 aaaaagacca gaccatcaaa agcttccaga gaactcggta gaaacgcctg tgcggccagt
     6481 ccgaacaaag taaatgccgg gaatgccgcc caccagttat cgcgatcatt gatcagtatg
     6541 tacagaaaaa gcgcgccagc ggccgcaaag atcaaacccc agaaaattcc gcccgcattt
     6601 gaaatcacgc ccatcgcatc caatagtgca agtaagcctc cgaataccag taaaacgcca
     6661 agccataagc gaggatcaaa tcttcttaat tccatcttca tactccttta taatttgctc
     6721 aagccgctgc cgagaactaa agttacttca catttaccga acccagcccg gattcaatat
     6781 tgatttctgc gcggtcgatc gctgtatcaa agtcggacga ttggtataag ctcgaactca
     6841 gacgcgggaa tcgattcgtg tccacgttga tcgcactcac gccttcttcc acatggattc
     6901 gcgcggcggt ggcttcaggg acgcgaatgt ttacggcagc ggcacccgat tcgacctcca
     6961 gaaatgacat gccgcgcgcg gggagagtca cgttggagct actggcgcct gtgctcaatt
     7021 cgatgcgcga ggcttgtaca tccaatagat taatatcgat ggaacttgcg cccgcctcca
     7081 cactcagcgt gaccggcacc tctttggtta attggaacct ccagatgccc tgcgaaggac
     7141 cgataaatgg aacgagcgag ggacccgctt ccacatggac ctcgagactc tcaccgttta
     7201 gccgattttt gtgattcatc cccatgccgg ttgtgccgac cagcgccgtg ctggcagacg
     7261 ttccaccact gatctcaatt tgtccggcac catgtgaaaa tttataatca acgcgctttg
     7321 ctgttccaag gggaatcgag aaggtctcgt ccggcgattg atccccgcgc caatacacgc
     7381 ttaagatcag ccaaccgccc accaggatga gtgtaacggg ccagaagaac gtgaaaacat
     7441 tggcaataat cccgctttgt tgaagaaaga gcaggaaacc aaacaggatc agtgccgctc
     7501 cccaaaagag gttatcgcgt ttcattttgt cctccacttc cactgcgcaa gaaaccgcgc
     7561 gcaaggacat ataagccaag caagatgaga atgaccgggg cgccatattc gcccagtata
     7621 ttcagcccac caaagaaggt cgcgaagacc aggaacaaaa ctgcactggt caccagaagg
     7681 cttgtgcctc gcgcaagatt ggcgcgggtg ctttcaccta acaaaccggc caggattgtg
     7741 ccgactccca caaagccggg aatgagtgtc cacatgtaag accaggagct gaaatcattg
     7801 gtcgcgtttt gataatagag aataccgcca atcccagcga tgatggaagc tggaacagcc
     7861 atgccgggtg ctccggtgat caaaccgatg agaagcacca acacaccggc gccgatcgtc
     7921 cacatgggcc aggcgaaatt actgtcgatc cattcctgaa gcgcggggat ttgtcgcgtg
     7981 gcaaacagcc agccaccgat caggattaga agaataccga gaaccagatt cgtacggttt
     8041 tgacgcattg gaagactctc ctttttgagg ttaattccat attacttaac gtcaaaacag
     8101 ccattaagtt gcttatttat gggacgggct ggggatcagt ctcaaagacg ataatatcat
     8161 tcggaatcca cttcttctgg cggataatgt caataaacgc gtaacgatag atcagccgag
     8221 ccgtaattgt cgcatttcca ttggcgggaa ccgcaaaaga atattcactt tgaaccggct
     8281 catg
//
LOCUS       DASTJP010000464         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1045164, whole genome shotgun sequence.
ACCESSION   DASTJP010000464 DASTJP010000000
VERSION     DASTJP010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045164"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFQ23_13485"
     CDS             <1..348
                     /locus_tag="VFQ23_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907658.1"
                     /translation="QWDQPMERIRLVDGDTELLPGLELIETNGHVPGHQSVLVRLPKT
                     GAVLLTIDAVPFAEGFTRDAQDDGSDPDAAATHASTIKLIDLVEQDHIGLVIFGHDAA
                     QWETLKKAPEFYD"
     gene            complement(415..657)
                     /locus_tag="VFQ23_13490"
     CDS             complement(415..657)
                     /locus_tag="VFQ23_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907659.1"
                     /translation="MPRKREIIVGKFYVSSDRRIAREVLSTSLQTVRFNTYHLDTGNS
                     CGTPSECLIQDFVRWATREASPKELAFLQNQGIETP"
     gene            1077..1340
                     /locus_tag="VFQ23_13495"
     CDS             1077..1340
                     /locus_tag="VFQ23_13495"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HET9907660.1"
                     /translation="MANEERRKLLEEFRNQVAQAESLDEEGRTLLLSLDKEIERLTDD
                     EAADTPDTVLEQLQESIERFEESHPTLTMTLSQIMTVLSNAGI"
     gene            1470..2162
                     /locus_tag="VFQ23_13500"
     CDS             1470..2162
                     /locus_tag="VFQ23_13500"
                     /inference="COORDINATES: protein motif:HMM:NF006976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET9907661.1"
                     /translation="MPKRHYPWLWFDADGTLFDYNLAEVTALKKTFESLRLRYEDDYL
                     KIYQEINHGLWQALERQEIRPDVLRVRRFQLLLANLKLDGIAEVMSTAYIEQLGLCTD
                     LINGAYEILDALHGKCRFAIVTNGLQAVQHSRLSLSKIHKFIDVIIISEEVGAAKPHS
                     AFFEIASERTGHPAKSEVLIIGDSLSSDIRGGVDYGIDTCWYNPAAAARPDALPITYE
                     IKHLKDLLEIVE"
     gene            2181..2414
                     /locus_tag="VFQ23_13505"
     CDS             2181..2414
                     /locus_tag="VFQ23_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907662.1"
                     /translation="MKNGTCPNCNSATVYTKRQGVSFASDDYFYVRISSERMSRSLKE
                     IDHYVCTTCGYLETYIEDKAKLDAIAKDWKKIG"
     gene            2417..2782
                     /locus_tag="VFQ23_13510"
     CDS             2417..2782
                     /locus_tag="VFQ23_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907663.1"
                     /translation="MKKMSPYWALIIGLLVVAAQIISFYVRFGRWNTNAFFLDYLLMF
                     LSGALGGLILIYFRNQQSSVDGRFIVTIAFLVASPLALIMMIAGGLLGVLGILLFPQI
                     PWALFSWIGSVVGKRVSRR"
     gene            complement(2885..3319)
                     /locus_tag="VFQ23_13515"
     CDS             complement(2885..3319)
                     /locus_tag="VFQ23_13515"
                     /inference="COORDINATES: protein motif:HMM:NF039385.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HET9907664.1"
                     /translation="MQVQIASDKTILKEGNLTVTDRRVTVGAKTYATANIASVRIQEN
                     EPRFFLPLFFMLITGICLALIAASDMQDLSYLLNWGMYLSVGAFFLLLFSQKTKYSVL
                     MRMRGSVDEMSLLQTPNRDYAEKVVKMLREAIKHGEKLSDDM"
     gene            complement(3770..3949)
                     /locus_tag="VFQ23_13520"
     CDS             complement(3770..3949)
                     /locus_tag="VFQ23_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907665.1"
                     /translation="MPVQHPTIEILLAITLSFSILLILFVAVGAAVASLLSEEQTKVE
                     PPLELELQPSDQTEL"
     gene            complement(4100..4360)
                     /locus_tag="VFQ23_13525"
     CDS             complement(4100..4360)
                     /locus_tag="VFQ23_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907666.1"
                     /translation="MKKCPYCSEEVQEEAILCRHCLRDLWPAPRRTFHNTRQLMSSFE
                     YVLSWLKVNDPDLYQTIRERLAEAENKPIPVRIPRKHLQTTD"
     gene            complement(4441..>5085)
                     /locus_tag="VFQ23_13530"
     CDS             complement(4441..>5085)
                     /locus_tag="VFQ23_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907667.1"
                     /translation="VGTGTGRISVPLLERGADLIGCDISSNMLARLKDKFPSARIAKA
                     DASLLPLPEAHFDVVLTVHVMHLIPPWRAALREFRRVLKPRGKYLNVRTWAPGGASIR
                     DELRHHWRQWVEAQGVDVRHVGIQDLSELLQELKVLDGSMSEIEVMRYTLPFTLREEL
                     ERFGARSSSETWDVPDVTFDASMSDLRAWVDQHFGDLDQQLEEEVRFVIDVVQF"
BASE COUNT         1318 a         1252 c         1265 g         1250 t
ORIGIN      
        1 caatgggatc agccaatgga gcgcattcgg ctggtggatg gggacacgga actattgccg
       61 gggctggagc tgattgagac gaacgggcat gtgccgggac atcaatcggt gctggtgcgg
      121 ctgcccaaaa caggggcggt cttattgacg attgacgcgg tccccttcgc cgagggcttt
      181 acccgtgacg cgcaggacga cggcagcgac ccggacgctg cagcgaccca cgccagtacg
      241 attaaactga ttgatctggt ggaacaggat catatcgggc tggtgatttt cggtcatgac
      301 gcggcgcagt gggaaacact caaaaaagcg ccggagtttt acgattaaat tagagcgcaa
      361 cgtgaaaagt ttagtggagc agggaaggta gtaatccttc aacgattcaa atgcctatgg
      421 agtttcgatc ccctgatttt gaagaaaagc cagttctttg ggcgaggctt ctctggtcgc
      481 ccaacgtaca aaatcctgaa tcaaacactc actgggtgta ccacatgaat tacccgtatc
      541 caaatgatac gtgttgaatc tgacggtctg caagctggtg gataatactt cgcgggcgat
      601 ccttcggtcg ctgcttacat agaactttcc aacgatgatt tctcgttttc gtggcatagg
      661 ctgctccaag cttaaagtgc gcacaagtac cgatgcgagt gcggattctc aggttgcgac
      721 agaggtgaca gattggaacc gggaaattat tttagccgcg ataaaattcc tgtggtgaaa
      781 ataacttaag tagtcaattt gtaaatatta atctgatcga tttctagcag atgacaaagc
      841 ccagcctttc ctaaaaaaca atcagattgg gggattttcc tggtggggta gtacagagct
      901 gatggaacga aaatttcgcg gctctaagta aaataccggt ggtgaaacgc gtgtcaaagg
      961 tcattctgta gaaaccccta cagactccct tcaggagagg gcgggggtga ggtcaaaacg
     1021 ctgtacaata tgagtagaca gaaattcttg catatcgcaa gaaggaggta tttcctatgg
     1081 cgaacgaaga aagacgcaaa cttttagagg aatttcgaaa tcaggtcgcg caggctgaat
     1141 ccctggatga agaggggcgc accctcctgc tcagcctcga taaggaaatc gagagactga
     1201 ccgatgatga agcggcggac actcccgata cagtcctcga gcaattgcag gaaagtatcg
     1261 aacgttttga ggagtcgcat cctacgctga caatgaccct ctcacaaatc atgaccgtgc
     1321 tgagcaacgc cggaatctaa aacaaggtat taacatcacg gtagatctgc taccgaggtc
     1381 acagagttct ttgggaaaaa atctcagatt ctctgtggtc tctgtggcta agtctattct
     1441 tttcaccaca tccttcctta acaaaatcta tgccgaagag acactacccg tggttatggt
     1501 tcgacgccga tggcacactg ttcgactata acctggcgga agtgaccgcg ctcaaaaaga
     1561 catttgagtc cctgcgcctg cgctatgaag atgattatct gaaaatctat caagagatca
     1621 atcacgggtt atggcaagca ctggaacgac aagaaatcag gccggacgtt ttacgcgtcc
     1681 ggcgttttca attgttactg gcgaatctaa aactggatgg cattgctgag gtgatgagca
     1741 cggcatacat cgagcaattg ggactctgta cggacctcat caatggcgcg tatgaaattc
     1801 tagacgctct gcatgggaaa tgtcggtttg cgatcgtgac caatggtcta caagccgttc
     1861 agcacagccg cctgtcgctt tcgaaaatac acaaattcat tgatgtaatt attatttcag
     1921 aagaagtcgg cgctgccaaa ccacattccg ccttctttga gatcgcctct gaacggacag
     1981 ggcatcccgc caaaagcgaa gtgctgatca tcggcgatag cctcagctca gacattcgcg
     2041 gcggcgtgga ttatggcatt gatacctgct ggtacaatcc agcagcggca gctcgaccag
     2101 atgcactgcc catcacatat gaaatcaaac acttaaaaga cttactggag atcgttgaat
     2161 aacaggccat aggagtttca atgaaaaacg gtacatgccc caactgcaat tcagctacag
     2221 tctataccaa acgacaggga gtcagcttcg ccagtgatga ctacttctac gtgcgcatct
     2281 ccagcgaacg gatgtcacgg tcgcttaagg agatagatca ctacgtctgc acgacctgcg
     2341 gttatcttga aacatatatc gaagacaaag ccaagctgga tgccatcgca aaggattgga
     2401 aaaaaatagg ctgaagatga aaaagatgtc tccatattgg gcgttgatca ttgggctgct
     2461 ggtagtcgcc gcgcagatca tcagcttcta tgtccgattt ggacgatgga acacaaatgc
     2521 cttcttcctc gattatctgc tgatgttcct gtccggcgcg ttaggtggct tgatcctgat
     2581 ctacttccga aaccaacagt cttcggtgga tggtcgcttc atcgtaacca ttgcatttct
     2641 ggtggcatct ccgctggcgc tgatcatgat gatcgctggt ggcttgctgg gggtcttggg
     2701 aatcctgctc tttcctcaaa tcccgtgggc gctgttttca tggattgggt ctgtggtggg
     2761 gaagagagtt tcgagaaggt gaagtacggt ttcggtttcc cacgctgact gaacaagaca
     2821 tggtagagcg aaaaaaaaca cccagccatt tggttgggtg ttcctgttct tttccattag
     2881 aggcttacat gtcatcggaa agtttctcgc catgtttgat tgcttcacgc aacatcttga
     2941 ccactttttc tgcataatcc cgattgggtg tttgcagcag gctcatttcg tccaccgagc
     3001 ctctcattcg catcagcaca ctatattttg ttttttgaga gaaaagcagc aggaaaaacg
     3061 ctcccacgct caaatacatt ccccagttga ggaggtacga aagatcctgc atatcggagg
     3121 ctgcgatgag agccagacaa atccctgtaa ttaacatgaa gaaaagcgga agaaaaaatc
     3181 tcggctcatt ctcctgaatc cggacggatg cgatattggc ggtcgcgtac gtcttcgccc
     3241 caaccgtcac gcgtcgatcg gtcaccgtta gattcccctc cttcaaaatt gttttgtcac
     3301 ttgcgatttg aacttgcata cataccttac ttactttctc actacatgtc taattgagac
     3361 attctccggc tcttaacccg aacacccctg ccagcaacca aaaaaacgga gagatgaagg
     3421 aagccaaaaa agcaaaaatt aacgaaatcg ccagatagct cggcccgagt tgtctgcgct
     3481 gctctttccg gcaatccttg cagacgtgcc catggagctc gtactttgta aaaaggctgg
     3541 tttcacagat tttgcatttc atacgctgat tttacaaacc ggaattttgc ggctccattt
     3601 cattaaatct tctttcaggc tgaatcataa acgccttcca tctggtgctg ggcccggtgg
     3661 aaggcgtttc ttgctaacct ggatgctcag gaagaaagtg gtggaagtcc tgagtgcccc
     3721 aggtacatcc attttacaga aacccttcgt gcgagaacat tgcaccttgt tacagttcag
     3781 tttgatcact gggctgtaac tccaactcca gcggcggttc gacctttgtc tgctcctcag
     3841 aaagtaggga tgccacagct gctcctacgg cgacgaacag gatgagcaga atggaaaaag
     3901 aaagtgtaat cgccagcagg atttcaatgg ttgggtgttg aacgggcatg atccatctct
     3961 taatagaata ttaattttca cctacaagac gccaccccgg gaattaagtt accattggga
     4021 aggttacact ctggaaagtt agaaaacctc ccatttcggg aggttttcgc tgagaaccta
     4081 tggtagctgg agccggattt cagtcggtcg tctgcaagtg tttacgcgga atccttacgg
     4141 gaattggctt gttctccgcc tctgccaatc tttcgcggat cgtctggtac aggtccgggt
     4201 cgttgacctt cagccagctc aggacgtatt caaacgagct catcaactgt cgcgtattat
     4261 ggaacgtacg gcgcggtgcg ggccaaagat cacgcagaca atggcgacag aggatcgctt
     4321 cttcctggac ttcttcagag caatacgggc attttttcat cttcggcgtc tgttgtcttt
     4381 cttgttaaca agacgacaat gaccgaacaa tattcattgc agatgaattg aaaacccgcc
     4441 ttaaaattgc acgacgtcga tcacaaaccg tacctcttct tcgagctgct gatcgaggtc
     4501 tccaaaatgc tggtccaccc aggcgcgcaa gtcactcatg ctcgcgtcaa aggtaacgtc
     4561 ggggacatcc cacgtctctg agcttgatcg agcaccaaag cgctccagtt cttcgcgtaa
     4621 agtgaaggga agtgtataac gcatgacttc gatctctgac atacttccat cgaggacttt
     4681 taactcctgc agtaattccg ataaatcttg aatcccaaca tgacggacgt ccacgccctg
     4741 cgcctccacc cattgacgcc agtggtggcg gagctcgtca cggatggaag ctcctcccgg
     4801 ggcccaggtc ctcacattga gatacttccc tctcggctta agcacccgcc tgaactcgcg
     4861 taaggctgca cgccagggag ggatcaggtg catcacgtgc acggtcaaca ccacgtcaaa
     4921 gtgtgcctcc ggcaagggta gcagcgatgc atccgctttc gcgatgcgcg ccgatgggaa
     4981 tttatctttt aatcgagcta acatattgga tgagatatca catccaatga gatcagcacc
     5041 tcgctctaaa agcgggacac tgattcgccc cgtgccagtc cctac
//
LOCUS       DASTJP010000465         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_969172, whole genome shotgun sequence.
ACCESSION   DASTJP010000465 DASTJP010000000
VERSION     DASTJP010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969172"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFQ23_13535"
     CDS             <1..737
                     /locus_tag="VFQ23_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907668.1"
                     /translation="IFFGHNLLDPIRGTGISTLDLIWYALHQPNTLFLGNSLISMVYP
                     VLPWLGLMAMGYGFGMLYRKDFNKVKRQRWLLVLGSGAILLFLVLRGFNLYGEPRPWS
                     PQNSSIFTIMSFLNTTKYPPSLHFLLMTIGPALMLLALSEGFRAGIAIPVIVFGKVPF
                     FFYVLHLYLIHSLAMLALILTGRDWHEYILSAGALGSGALNNFGFSLGTVYVIWIGVI
                     VILYPLCQWYQKYKEHHPSAWWLSYV"
     gene            804..1712
                     /locus_tag="VFQ23_13540"
     CDS             804..1712
                     /locus_tag="VFQ23_13540"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HET9907669.1"
                     /translation="MEHQSKHRNTTYITSELKTEEEINLDNQGLFSVPNEVQQLTNLK
                     SLSLYKNELTDLPETIWQFTDLEVLNIADNKFSDLPEAIGNLTNLRMLDAGHNALTRL
                     PDALGKLASLKFLYLSNNHLSSVPRSLEYLQKLIYLNITDNLLPSLPENIGKLTSLIE
                     LRLYNNEIRSIPDDIENLIHLRELHIMNNQLTSLPASIGKLKNLKKLILQGNAIEALP
                     NSIGDVSGLTDLDLRFNQLTEIPETIGKLTHLRFLDLRANQLSTLPSIMVNLKNLEKL
                     DLRWNKFQSIPDWFSGLEQHGCVIYT"
     gene            1838..>2863
                     /locus_tag="VFQ23_13545"
     CDS             1838..>2863
                     /locus_tag="VFQ23_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9907670.1"
                     /translation="MKNLLSYSSALIVLSFIATACGASPVTSAPVATDVPPSTSTPLP
                     LPTALSTETLIPTAIPTVTPVPFPLQIIAMRAREYPGSDVVIESVLDPGVNYNRYYVS
                     YLSEGLKIYALMTVPNGERPETGWPVIIFNHGFIPPDVYVSTERYIAYVDLIARSGYI
                     VFRSDYRGHGNSEGEAGGAYSRPDYTVDVLNAVSTMKRFPEADPNRIGMWGHSMGGYI
                     TLRSMVVTGDIKAGVIWAGVVASYPDLLYRWRRGTSASVPTTPRPGSWRTTLVETYGS
                     PEENPEFWNSISANSYLADLSGPIQLHHGTLDEDVPLEFSETLFYQMLEAQQYVELYK
                     YDGDNHNI"
BASE COUNT          753 a          682 c          606 g          822 t
ORIGIN      
        1 ttatcttttt tgggcacaat ctcctggatc cgattcgagg gactggcata tcaacgctcg
       61 atcttatctg gtatgcgttg catcaaccaa acaccctgtt tttaggtaat tcactcatca
      121 gtatggttta cccggtgctg ccgtggctgg gactcatggc gatgggatat ggatttggaa
      181 tgctttatag aaaggatttc aacaaggtta aaagacagcg atggttgctg gtccttggct
      241 caggtgcgat attgctgttt cttgttcttc gcggattcaa tctgtatggc gaaccgagac
      301 cctggagccc gcaaaattcc tcgatattta cgattatgtc atttctgaac acaacgaaat
      361 atcctccatc tctacatttt ctacttatga cgataggacc tgccctgatg ctccttgcgc
      421 taagtgaggg ctttcgggct ggaatcgcaa ttccagttat tgtatttggc aaggttccat
      481 ttttctttta tgttttacac ctttatctaa ttcattctct tgcgatgctc gcacttattt
      541 tgacaggacg cgattggcac gaatatatcc tttcagcagg cgcgctggga tcgggcgcct
      601 tgaataattt cggatttagc ctcggcacgg tttacgtcat ctggattggg gtgatcgtta
      661 tactctatcc actttgccag tggtatcaga aatataaaga acatcacccg tcagcgtggt
      721 ggttgagcta tgtatgatgt gtagtttctg aagattaaat tcaaggttta gacgggattg
      781 tattttgcgc tagaataaca tctatggaac atcaatccaa acaccgcaac accacgtata
      841 ttactagtga gctgaaaact gaggaagaaa taaacctgga taatcagggc ttattttctg
      901 tcccgaatga agtgcaacaa ctcacaaatc tcaagtccct cagtttatac aaaaatgaat
      961 tgaccgattt accggaaacc atctggcagt tcacagacct tgaagttttg aatatagccg
     1021 ataataaatt ctctgatctt cccgaagcga ttggaaatct tacgaacctg cgtatgctgg
     1081 atgcaggtca taatgctctg acacgtttgc cagacgcgtt aggcaaactg gcatcgttga
     1141 aatttcttta tttgagtaac aatcatctta gctccgttcc ccgatcgctg gaatatttgc
     1201 aaaagctcat ttatctcaat atcacggata atctcttgcc ttcgcttcct gaaaatatcg
     1261 gaaaattaac gagcttgata gaacttagac tctacaataa tgagatacgc tccatccccg
     1321 atgacatcga aaatttgatt caccttcgtg aacttcatat aatgaacaat caattaactt
     1381 cgcttcctgc atccattggc aaattgaaaa accttaaaaa actaatactg cagggaaatg
     1441 ccattgaagc actgccaaat tccattggcg atgtatctgg ccttactgac cttgacctac
     1501 gattcaatca attgacagag atcccagaga caatcggcaa gctaacacac ctcaggtttt
     1561 tagatttgag agcaaatcaa ctctctactt tgccgtcaat tatggtcaac ctgaaaaacc
     1621 tggaaaaact tgacttgcgc tggaacaaat ttcagtcgat accagactgg ttctccgggc
     1681 tcgaacagca cggttgtgta atttacactt aagcccaacc ttttttacca tcgtctcatc
     1741 gtttactaaa ctacttaaag taaattgtag gataagcttt tgtgttaatt tgtgctaaat
     1801 cgaaatacct ttacctgcgc aacacgagga ttaccccatg aaaaatttac tgtcgtattc
     1861 atcggcgttg atcgttttat cgttcatcgc cactgcctgc ggggccagcc cggtaacttc
     1921 tgcacccgtc gctactgatg ttccaccgtc aacatctacg ccacttccgt tgccaactgc
     1981 actttccact gaaactctca ttcctacggc aatccctaca gtcacacctg ttccatttcc
     2041 gctacaaatc attgccatgc gcgcccggga ataccccggc agtgatgtag tgatcgaaag
     2101 cgtccttgac ccgggtgtca attacaaccg ctattatgtt tcgtatctct ccgagggctt
     2161 gaaaatctat gcgctcatga cagtaccgaa cggtgaaagg ccggagaccg gatggcccgt
     2221 catcattttc aatcacggat ttattccacc cgatgtgtac gtctccacag agcgatatat
     2281 cgcctatgta gatttgatcg cccgcagcgg ctatattgtc tttcgttctg attatcgagg
     2341 ccacgggaat tcagagggcg aggccggagg tgcttacagc cgacccgatt acaccgtgga
     2401 tgtcctcaac gcggtttcaa cgatgaaacg attcccagaa gcagatccga accgtattgg
     2461 gatgtggggc cattccatgg gtggatacat cacacttcgt tcgatggtgg tcacaggtga
     2521 catcaaagca ggtgtgattt gggccggggt tgtcgcatcc tacccagacc tactgtatcg
     2581 ctggcgacga ggaaccagtg cttccgttcc aacgaccccg cgcccaggtt cctggcgaac
     2641 gacgttggta gaaacgtacg gttcgcccga agagaaccct gaattttgga attcaatttc
     2701 ggctaattcc tatcttgcgg atctatcagg acccatacaa cttcatcacg ggactttgga
     2761 tgaagatgtg cctttggaat tttcagaaac gcttttttac cagatgcttg aagctcaaca
     2821 atatgttgag ttatacaaat atgatggcga taatcataat atc
//
LOCUS       DASTJP010000466         4369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_882324, whole genome shotgun sequence.
ACCESSION   DASTJP010000466 DASTJP010000000
VERSION     DASTJP010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882324"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="VFQ23_13550"
     CDS             <1..1703
                     /locus_tag="VFQ23_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9907671.1"
                     /translation="WQKELEDSFPYVETDDQKRALADIKRDMESTRPMDRLLCGDVGY
                     GKTEVALRAAFKAVMDGKQVGILVPTTVLAQQHYETFLQRLAAFPVKVEMLSRFRTAR
                     EQTSIIHGLAIGEIDIVIGTHRLISSDVQFKELGLVIIDEEQRFGVTHKEHLKKLRTE
                     VDVLTLTATPIPRTLYMALTGVRDISNLNTPPEERLPIVTHVGPYSPKLVRQAILREL
                     ERGGQIFFVHNRVNTIDAMKVHLSQLVPEAQVDIGHGQMPETQLADVMHRFNAGETDV
                     LLSTTIIESGLDIPNANTLIVDRADTFGLAQLYQLRGRVGRGAARAYSYFFRHGKLSP
                     TQEGQQRLEVIAENTQLGAGYSIAMRDLEIRGAGDLLGTRQHGHIQAVGFHLYTRLLA
                     DAVRQIRRIEAAKKNGGSGEESVKMDSVFANLTQPISMPVNVDLPLAVGIPAAYIADQ
                     DLRLRLYRRIADLRDETEIDALVSEFQDRFGELPEMVENLFYQMRVKLRAEKAGLSAI
                     NWESGQIVLRYPASSEEKEGRRLVDLGPGIRGGRNAYWCSFGENWQVKLLEALEMIKP
                     "
     gene            complement(1808..2071)
                     /locus_tag="VFQ23_13555"
     CDS             complement(1808..2071)
                     /locus_tag="VFQ23_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9907672.1"
                     /translation="MGLISWIIVGLIAGWLANQMMRSGRGNAVTDIVVGIIGGLVGGF
                     LAGELFGVPNAVNGINITSIIVAFLGAIAAIAIVRALPGRSPL"
     gene            complement(2302..2844)
                     /locus_tag="VFQ23_13560"
     CDS             complement(2302..2844)
                     /locus_tag="VFQ23_13560"
                     /inference="COORDINATES: protein motif:HMM:NF020544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HET9907673.1"
                     /translation="MSVEEKLIRLSTSDFASLQIQPGALSDLIEDLYGADYFDSRSVY
                     KRPVNFDIPAPHMIHIDEFTIWLLTDRNELTALCDAVAAWRDNQELIGAQHGQGFAYA
                     TFQSPEAVSAIARGLEIYPQEELSKRFFSQAESFRQAAAPYFGEDEAILAYQRDFFEL
                     LHKFYQEASSGREFVLHLIV"
     gene            complement(2893..3774)
                     /locus_tag="VFQ23_13565"
     CDS             complement(2893..3774)
                     /locus_tag="VFQ23_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9907674.1"
                     /translation="MNTAATRQKRQDLMVFIEQELVSEPAVQAVVGIGSIANGLARPD
                     SDIDAIVFLDPFDWYVTPAEFLWNPAENTFHSIFSEAPSKERWMQFDFARLDLARWAA
                     PSFEWSEERCAELQEGWLAFDRSGRVATLIETRTAYPNAIRISKLDEAITWLDQHLSD
                     DGVQARWESLGPVIAYDRLQAAYEYLVQALFAYNRRWRPWRNREMSSLLALAWLPDHF
                     ADQVPGALGASSIDYAGYMSRVHCLRGLFQDITQRLVSEGEYGVDIIGEAFIRSHDEP
                     GRAWNMNEWNRKHAIGR"
     gene            complement(3884..>4369)
                     /locus_tag="VFQ23_13570"
     CDS             complement(3884..>4369)
                     /locus_tag="VFQ23_13570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907675.1"
                     /translation="DRQVAEREGLVIKTIEGDAADLSVFANESFDLIFNPVSTVFMPD
                     VRAVWKECSRVLRQGGVLLTGFMNPVHYIFDLYKADEGVMEVANSIPYSDLTSLPKEY
                     LDELIEKGLPLEFGHSLTDLIGGQCDAGLAITDMYEDYMLESLVHNYHPNYMATRAKK
                     S"
BASE COUNT         1133 a         1116 c         1065 g         1055 t
ORIGIN      
        1 aatggcaaaa ggaactggaa gattcattcc cctatgtgga aacggacgat cagaagcggg
       61 ctcttgctga tatcaagcgc gacatggaat ccacacgtcc gatggatcga ctgttatgtg
      121 gcgatgtcgg atatggcaaa acagaagttg cgttacgcgc ggcgttcaaa gcagtaatgg
      181 acggcaagca ggtcggtatc cttgtgccaa cgactgtact ggcgcaacag cactatgaga
      241 ccttcctgca gcgactggcc gccttcccgg tcaaagtgga gatgctctcg cgctttcgaa
      301 cggcccgcga gcaaacttca atcatccatg gtctggcaat cggcgagatc gacatcgtta
      361 tcggaacgca tcggttgatt tcatctgacg tgcaattcaa ggaacttggg ctggtcatca
      421 tcgacgaaga gcagcgcttc ggtgtgacac acaaagagca tcttaagaag ctccgcaccg
      481 aagtggatgt gcttacgctc accgcaaccc ccatcccgcg cacgctttac atggcactga
      541 ctggcgtacg tgatatttca aacctcaaca cgccgccgga ggaacgattg ccgattgtga
      601 cgcatgtggg tccgtattcg ccgaagcttg ttcgtcaggc gatcctgcgt gagttggagc
      661 gcggtggcca gatcttcttt gtacacaacc gcgtcaacac cattgatgcg atgaaagtcc
      721 atttgagtca gcttgtgcct gaggcgcagg tggatatcgg gcatgggcag atgcccgaga
      781 ctcaacttgc ggatgtgatg catcgcttca acgccgggga gacagacgtt ttactttcga
      841 cgacgatcat cgaatcgggc ctggatattc cgaacgctaa cacgctcatc gtggatcgcg
      901 ctgatacgtt tgggctggca caactttacc aactgcgggg tcgagtcggg cgaggtgccg
      961 cgcgtgccta ctcctacttt ttccgtcacg gaaaattgag ccccactcag gaggggcagc
     1021 agaggctgga agtgatcgcc gaaaacacac agcttggagc aggatactcc attgccatgc
     1081 gcgacctgga aattcgcggc gcaggggatc tgttgggcac gcgccagcat ggacatatcc
     1141 aagccgttgg gtttcatctc tatactcgct tacttgcaga tgcagtgagg caaattcgcc
     1201 gaattgaagc tgccaagaaa aatggtgggt caggcgaaga aagcgtcaaa atggactctg
     1261 tgtttgccaa tctcactcag cccatctcca tgccggtcaa tgtggatttg ccactcgcgg
     1321 tgggaatccc cgcagcttac attgctgacc aggatttacg tttgcggctt tatcgtcgca
     1381 ttgctgatct gcgcgatgag acagagatcg atgccctggt ttctgaattc caggatcgtt
     1441 ttggtgaact gcctgaaatg gttgagaatt tgttctacca gatgcgtgtc aaattacgcg
     1501 cggaaaaggc tggcctgtct gccatcaatt gggaaagtgg gcagatcgtg ctgcgatacc
     1561 cggcttccag tgaagagaaa gaggggagac gactggtgga tttaggcccc ggtatacgag
     1621 gcgggaggaa tgcctattgg tgttcgtttg gcgaaaattg gcaggtcaag ctgctggaag
     1681 ctctggagat gataaagcca taagcagggt ttttatcgac caatggactg tctctccaag
     1741 tgaattccca gagagaatca aaactgggtt gccgaaggca acccagtttt ttcattccat
     1801 agtaacacta aagcggggaa cgcccgggta aggcacggac aatcgctatg gctgcaatcg
     1861 cacccaaaaa tgcgactatg atactggtaa tgttgattcc gttcactgca ttcggaacgc
     1921 caaacagttc tccagccagg aagccgccta ccaggccacc gatgatacca acaacgatat
     1981 cggtgactgc gttaccacgg ccgctcctca tcatctgatt ggcaagccaa cctgcgatga
     2041 gccccactat aatccaagat ataagaccca tttcgtattc tccttttgta acttattgtt
     2101 gagcgcgtgt cgtagcattt cgctctacta taaattctaa cgtgaatata aaaatcatgt
     2161 tatgggggat aaccctaaag gatttgctgc gtgtaaacat caagagtact tgatacgtat
     2221 tctgagcttt agctatccgc gcgaaaaata actgtagtaa gtttatgaaa acttttgatt
     2281 cttacgggaa gtcaaattcc actaaacaat gagatgcagc acaaactctc gtcccgacga
     2341 tgcctcctgg tagaacttat ggagaagttc aaagaagtcc ctctgataag caaggattgc
     2401 ctcatcttcc ccgaaatagg gcgcggctgc ctgacggaac gattccgcct gcgaaaagaa
     2461 ccgctttgat aactcctctt gtgggtagat ctccagcccg cgagcaatcg cgctcaccgc
     2521 ttcgggactc tgaaacgtag catacgcaaa accctgacca tgttgcgcgc cgataagttc
     2581 ctgattatcg cgccaggctg caactgcatc acataacgca gtaagttcgt tgcgatccgt
     2641 taataaccag atcgtaaatt catcgatgtg aatcatgtgc ggtgccggaa tgtcaaagtt
     2701 aacgggcctc ttgtagacac tgcgtgaatc aaaataatcc gcgccgtata aatcttcgat
     2761 gagatcgctc aacgccccgg gttgtatttg caaagacgcg aagtctgaag tggagaggcg
     2821 gatcagtttt tcttcaacgg acatacagcc tgttttatca ttatcacaat cctatcaaca
     2881 tgacctttca tgctagcgac ctattgcatg ctttctattc cactcgttca tattccacgc
     2941 acgcccaggt tcatcatgac tgcggatgaa tgcttcacca atgatatcca ctccatactc
     3001 accctcactg actagtcgct gggttatatc ctgaaacaaa cctcgcaaac aatgaacccg
     3061 gctcatatat ccagcataat caattgatga agctcctaat gctcctggaa cttgatcagc
     3121 aaaatgatcc ggcaaccatg ccaatgccag caaagaagac atttcccggt ttcgccaggg
     3181 acgccaacga cggttatatg caaatagagc ttgcaccaaa tactcatagg cagcttgcag
     3241 acggtcataa gctatgaccg ggccaaggct ctcccaacga gcctgtactc catcatccga
     3301 taaatgctga tccaaccatg ttattgcctc atccagtttg gatattcgta tggcattggg
     3361 gtaggcggtg cgtgtttcta tcaacgtagc aacccggccg gatcgatcaa acgcgagcca
     3421 gccctcctgc agttctgcac aacgttcctc agaccattca aaggaaggtg cagcccatcg
     3481 tgccagatcg agacgggcaa aatcaaactg catccatctc tcttttgagg gcgcttcgct
     3541 gaaaatgcta tgaaaagtgt tctctgctgg attccatagg aattcagcag gagtgacata
     3601 ccaatcaaat ggatcaagaa agacgatggc gtcaatatca gaatctggtc gagctaaccc
     3661 attggcaata ctgccgatgc ccacgacagc ctgcaccgct ggttcggata caagctcttg
     3721 ctcaatgaaa accatcaagt cttgtcgttt ctgacgggtt gctgcagtat tcaaaattac
     3781 ctcattgaat ttcttaatat ggtcgtgatg ccagcggctg tcaaaagtat gcgtcgttaa
     3841 atactcttcc aacatcgatg gccaaccgcg agccaatcaa gtttcatgat ttctttgccc
     3901 gcgttgccat atagttggga tggtaattgt gcaccagcga ttcgagcata tagtcttcgt
     3961 acatgtctgt gatggctaaa cccgcgtcac attgaccgcc gatcaaatcg gtgaggctgt
     4021 gaccgaattc cagcggtaat cctttttcga taagctcatc gagatattct ttgggcaggc
     4081 ttgttaagtc tgaatagggg atcgagtttg caacttccat tacaccctca tcagctttgt
     4141 ataaatcaaa gatatagtga accggattca taaagccggt cagtaacacg ccaccctgac
     4201 gaagcacccg agaacattcc ttccacaccg cgcggacatc aggcataaag accgttgaga
     4261 cagggttaaa gatcaggtca aacgactcat ttgcgaaaac cgataaatct gccgcatcac
     4321 cttctattgt tttgataacc aacccttctc tttccgcaac ctgtcggtc
//
LOCUS       DASTJP010000467         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_718727, whole genome shotgun sequence.
ACCESSION   DASTJP010000467 DASTJP010000000
VERSION     DASTJP010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718727"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFQ23_13575"
     CDS             <1..854
                     /locus_tag="VFQ23_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907676.1"
                     /translation="TGKPVVIYFIGYPAPARKIGNLHFAISLSETAELAVNGNVESTP
                     EQVLPIPKAYLRGLFSGGTLAYETLLGLQASLSPIYSNAPITAHQLLKDPLHSEYHTI
                     IDLGDEFFMVGRLHPMIDNDLRIRRMKQEAADAQVRMILFDVVLGEGSHMNPAGELIP
                     AIKEILEERPGELEFVSIVIGTDDDPQNVQSQIDQLKDAGVIVFRTVTEAVEYISLRF
                     GASKESKDALIDLSQLNQPLAAINVGLESFHDSLTSQGALSVHVDWRPPASGNERLAS
                     LLAKMKK"
     gene            905..2149
                     /locus_tag="VFQ23_13580"
     CDS             905..2149
                     /locus_tag="VFQ23_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HET9907677.1"
                     /translation="MDIDHANTTAVNRMMEARPILKAVAAARDVIPAMKDNLFLHAGP
                     PIEWARMSGPLRGAIIGAMLFEGLAADETQAIAMAEQGEVEFDSCHHHGAVGPMAGVI
                     SASMKVYVVENVEHGNKSFSNLNEGYGKVLRYGAYSEDVLKKLRWMNDVLGSALADAL
                     AQSNGIDLRALIAEALHMGDEGHNRNKAGSLIYLKLLSPLIAKVVKDDAMEAEVLQFI
                     GDNALSVLNPVMAACKAMTDAAHGIECSTIVTSMARNGTDFGIRVSGLGEKEWFTAPA
                     EIPVGLFFSGFTQDDANPDIGDSAITETAGIGGFAMATAPAIVTFVGGTPKDAMNATL
                     EMYEITFAESKFFTMPSLDFRGTPTGIDLRKVVELGITPRINTGIAHKNAGVGQVGAG
                     LVRPPLKIFEDALVAYAEKYQL"
     gene            2193..2678
                     /locus_tag="VFQ23_13585"
     CDS             2193..2678
                     /locus_tag="VFQ23_13585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9907678.1"
                     /translation="MPTSLEDVRVLKNVEQVADAIFKIACRWDEFAQDVVGKQIARAA
                     DSIGANIAESFGRFHFGEKIQFLYYARGSVFETKYWLNRAATRGLMQSTDSQNYVMHL
                     TDIARQLNLYVSSLKGQRSGEITVAKTVKESAVEYVTSRLPDEFPSILFDDTDLAWLE
                     S"
BASE COUNT          743 a          681 c          790 g          687 t
ORIGIN      
        1 ggacaggcaa acctgtcgtc atctatttca ttggttatcc cgcgcctgcg agaaaaattg
       61 gcaatctgca ttttgcaatc agtctgagcg aaacggcaga gcttgccgta aacgggaatg
      121 tggagtccac acctgaacaa gtattgccga ttcccaaggc ctacctgagg ggtttgtttt
      181 caggtggaac acttgcctat gaaactctgc tcggtttaca ggcaagtctt tccccaattt
      241 actcaaatgc gccaattaca gctcaccagt tactcaagga tcctctgcac agtgaatatc
      301 acaccattat cgacctggga gatgaattct tcatggttgg gcgcctgcat ccaatgatcg
      361 acaatgatct gcgaattcgc cgtatgaaac aggaagccgc ggacgcgcag gtgaggatga
      421 tcttgtttga tgtcgttctc ggggaaggtt cccatatgaa ccctgcggga gaattgatcc
      481 ctgcgatcaa ggaaattcta gaggagagac ccggagaatt ggaatttgtt tcgatcgtga
      541 tcggcacaga cgatgatccg cagaacgtgc aatcgcaaat tgaccagtta aaggacgcgg
      601 gcgtgatcgt tttccgcaca gtgaccgaag ccgtggaata tatcagtttg cgatttggtg
      661 caagtaagga gagtaaagat gccctcatag atctaagtca gttgaaccag cctcttgctg
      721 caatcaatgt tgggctcgaa tcctttcatg acagcctgac ctcccagggc gcgttgtccg
      781 ttcacgtgga ctggcgcccg ccagcaagcg gaaacgaaag actcgcttca ctactcgcaa
      841 agatgaaaaa gtaataggta agtagcaatt cctatttacc cattacagat gctaggagat
      901 aaatatggat attgaccacg ccaataccac tgctgtcaat cgaatgatgg aagcaaggcc
      961 aatcttaaaa gccgtggcgg ctgcgcgaga tgtgatccct gctatgaagg acaacttgtt
     1021 cttgcacgcg ggcccgccga tcgagtgggc gcggatgtcc ggtccgctgc gtggggcaat
     1081 tatcggggcg atgctgttcg aggggttggc agcagatgaa acgcaggcaa tcgcgatggc
     1141 cgaacagggt gaggtcgagt tcgattcgtg tcatcatcac ggcgcggtcg gcccaatggc
     1201 gggagtcatt tccgcttcga tgaaagtcta tgtggttgag aacgtagagc atgggaataa
     1261 atcattctcc aatcttaacg agggttatgg aaaagtcctg cgctacggtg cgtatagtga
     1321 agatgtgctt aagaaactgc gctggatgaa tgatgtgttg ggcagtgcgc tggcagacgc
     1381 gctcgctcaa tccaatggca ttgacctgcg ggcgctgatt gccgaggccc ttcacatggg
     1441 cgacgaggga cataaccgta acaaggcggg ctcgctgatc tacttgaagc tgctctcccc
     1501 gctcatcgca aaagttgtca aagacgatgc aatggaagcg gaagtcctgc aattcattgg
     1561 tgacaacgcc ttgagtgtct tgaacccggt catggcggca tgcaaggcga tgactgacgc
     1621 ggcacacggt attgaatgca gcacgattgt caccagcatg gcgcgcaacg gcacagactt
     1681 tgggatccgt gtgagtgggc tgggagagaa ggaatggttt accgcccctg cggaaatccc
     1741 ggttggtttg ttcttttcag gctttacaca ggatgatgca aatcctgata ttggcgatag
     1801 tgccatcacc gaaaccgcgg gcattggcgg ctttgccatg gcgaccgcac ccgcgattgt
     1861 cacgttcgta ggtggcacgc cgaaagacgc gatgaatgca acactggaaa tgtatgagat
     1921 cacatttgcg gaaagcaaat tcttcaccat gccctccctc gatttccggg gcacgccgac
     1981 aggcatcgac cttcgcaaag tcgtcgagct gggaattaca ccgcgtatca atacaggtat
     2041 cgcacacaag aatgctggtg tgggtcaagt aggcgcggga ctggtgcggc cgccgctgaa
     2101 gatttttgag gatgcgttgg tggcatacgc ggagaaatac caactgtaaa aagagaatta
     2161 gagaagtaga ggattagacg ttggaggtta atgtgccgac atcgttggaa gatgtgagag
     2221 tcttgaagaa tgttgagcaa gtcgcggatg caatattcaa aatagcctgc cgttgggatg
     2281 aatttgctca agatgtggtt ggcaagcaaa tcgcccgcgc cgccgattcg ataggagcca
     2341 atattgctga gtcatttggg cgttttcatt ttggggagaa aatccaattt ctgtattatg
     2401 cccgtgggag tgtgttcgag acaaagtatt ggttaaaccg agcagcaaca cggggtttga
     2461 tgcaatccac tgattcgcag aattatgtta tgcacttgac cgatattgcg cgacaactta
     2521 atttatatgt ttccagtctc aagggtcaac gatctggtga aatcacggtt gcaaagactg
     2581 tcaaagaaag tgctgtcgag tacgtcacat cacggctccc tgatgagttt ccgagcattc
     2641 tctttgacga tactgacctt gcttggctcg aatcataacc attcaattct tcaatctcta
     2701 attctcaaat tgacaaagga gttccccatg accaaaatct acgacctatc ccaagacctc
     2761 aaccaggatt gttccttctg gccgttctac ccgccgttcg aggtgaaata catcaaacgc
     2821 aaatcggagc agggagtcaa tgcacagtat atccagactt cgaatcacat gggcacccac
     2881 ctcgatgcgc cgcggcattt t
//
LOCUS       DASTJP010000468        12736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_207631, whole genome shotgun sequence.
ACCESSION   DASTJP010000468 DASTJP010000000
VERSION     DASTJP010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207631"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFQ23_13590"
     CDS             <1..655
                     /locus_tag="VFQ23_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HET9907679.1"
                     /translation="DGIEMQTGMEGGLFSGFKRAVTGESFFITTFMNNDQRRRHVSFA
                     APYPGKIIAFDLDQFNGKVLCQKDSFLCAARGTEIEIAFTKRLGAGFFGGEGFILQKL
                     TGNGMVFVHAGGTIIEKELGAGETLRVDTGCIVAFATTVDYDIQFVGGFKNALFGGEG
                     LFLAKLTGPGKVYLQSLPFSRLVDRIKRGMTFTQSGGEQRGVAGIGGDIIKNIISGS"
     gene            849..6230
                     /locus_tag="VFQ23_13595"
     CDS             849..6230
                     /locus_tag="VFQ23_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET9907680.1"
                     /translation="MTTIKVLGNRNESLKQVSFGKELEGFEVSDAIQFSTDRSVSAYQ
                     VIDKLKANDLIELTFEGDIHRWVTVEELERDYKNQLSRGSEQGVLEIPALLPTGDASR
                     GATALALKILRVIKFDPVEEGAKKFAEFWDKRIMREPGLYRFDRGLDNSKKSGKIEHL
                     KMKQGKPILLFIHGTFSETASGFGGLTPEVWTLLQEHFGSEIYGYDHYTLSKSPIENA
                     LDLVKKLPPNVKLHIVTHSRGGLIGELLCRSGWKGKHGPFDDIDRKLMGENAKMASDL
                     DELSAVLKTKKISVERFVRVACPSRGTTLASKQVDRWLEILINVFGKLVPAGTPWAMG
                     YGVLTDLLLDFKKQGMNPEAIPGIAVMNPESNFIKMINRLDVELDVDLSVIAGDVEKT
                     GVIGQLATFFTDRFYSGDHDLAVQTRQMYGGPTRKNGRYFFHKGPGVWHCSYFSLKRT
                     AEIIKDALTLEPKKLEDRGFRPLEEAYARAVPEFQVQLTSRSFQKRSNLPQPVVYVLP
                     GVMGTHLAERGDRVWLDVADMAQGKMVNLNINSKSVEAESLVHLAYGNLVEYLSATHE
                     VIPFPYDWRKSILDEGNRFGEALEAKLKTLKETKSDQPIRILAHSMGGLVTRAMIALR
                     PKLWQEICKHNGARFVMLGTPNKGSYMIPRLILGRERIFRMLAMLDIKNSSEQLLELI
                     VRFPGILQLLPMDDPKWNFTEAKTWDIFPNLAKHKWVKPREEDLKHARDFHALLAKEE
                     NKFKEDDPVIYVAGYDRGAPVALDFDEKNEFIFRGTNQGDGSVPWGSGILPELEKDKT
                     YYMPVPHGDLPNHTDSFAALYDLLTDGRTTRLSKTPPRYDRGQEDYYPLRDEGVEIYP
                     SQLDLETTVLRITHAAQTVLSAKPVRVSVVHGNLSFSEHPVAVGHYEGDSLYSAERHL
                     DHYLDGKLSARHQLGLYPGPDGTAEVVLNDRGKKPGGAIIVGLGKAGELSPHRLTSSF
                     ANALREFGVKSVENEKVGEDGELKISTLLIGTGGMGLTVTSSIDAILAGVIQANKSFS
                     QLAETGQGENQRFNNIRIAEIKFIELYKDQAILAIKALNNPLLNRDGFDIKPRLEKMQ
                     GGWQRAAYEEPAGWWPRLYVRAGENADSSLVFSVPTARARSEESLLDIQRRNFDRLIA
                     QAVKNPNWDQALATAMFELMIPNRLKSSFKDLTNVLFVVDEKAAGYPWELLYDRRTGQ
                     EKPLVIQMGMIRQFSTATFQERVIDVTNKNIMVVGNPADTPDNFPNLPGAEQEGRLVA
                     EKFKEFQYDVRAAIYADSSTIMSDLFSQDYRVLHLAGHGVFEYEYKDKEDSEPEVFTG
                     MVLGNGVFLTANEIQNKMHIPELVFINCCHLGKISSRSKQPPDYAYNEFAASLSRKLI
                     DMGVKAVIAAGWAVDDAAALTFAEVFYDHLLKGASFGESVKSARQETYDLHKDRTNTW
                     GAYQCYGDPDYRFAASSSGGQRSKEKFVDVEEVILAIRRQHEISKTTAVLGLKKIRND
                     LRNLQKEIKEQNESWLEDALVADAFGAAFAEAFCFEEAIESYELATGSGKSTAAIKAL
                     EQTVNCRIRLAVQNVQTDPRNYGESIKSIKEQIRKLIRLMDAIGETPERLSMVGGGYK
                     RLARLTRDKSPDESASALDKMEKYYARAWDFNKEEVYPLTNLALAKIIRALWNGKPSA
                     KQLSEIEAQIKEARRLAVIEQKRSPDDFWSAMGLTDIKMLEHLLKYLKGSQRSFSEKV
                     HDELVEEYKKAWGQYGSAREINSVIEHYAFLLAVIKKPGSQKDVHKALHNVLDNILDS
                     LTATYEKVG"
     gene            6274..6846
                     /locus_tag="VFQ23_13600"
     CDS             6274..6846
                     /locus_tag="VFQ23_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907681.1"
                     /translation="MEKVWQVIRAIGLPIVVLLSGAMVMSILSGHGYVMEQLRENADS
                     KDRKPLNMRILGYDTNDVARHWAALDERALQSERRFLELDLIFPTFYGAALAFALWQA
                     WRVTGFVFSPAWVIVPVAITIIADWTENLIHLSQLHRYIESGEAGLQTASIQIASGAT
                     IVKLAFFAGILLFSAWKSVQNFQSSSVRRP"
     gene            complement(6890..7411)
                     /locus_tag="VFQ23_13605"
     CDS             complement(6890..7411)
                     /locus_tag="VFQ23_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907682.1"
                     /translation="MTSVEETNQSQANILCKSCGLCCTGHLFIWSKLKASELDSAESL
                     GLNVFRSVPSQRGFSQPCPLWQGVCTIYTSPHYPQFCRTYKCKLLKQLLDETTSLPDA
                     LAVIGQAKASIHELEMNLPVSPNPNFRERLVAQLEENSDEGFRLKARELLSLYENVFG
                     VKDLFDESFEDEK"
     gene            7520..8935
                     /locus_tag="VFQ23_13610"
     CDS             7520..8935
                     /locus_tag="VFQ23_13610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET9907683.1"
                     /translation="MDLSEWIESNRSMFIELSNEVWGYAELGYKEFESSKTLVDAFEE
                     AGFTLERGVADIPTAFVATYGNGEGPVIGILGEFDALPGLSQDCVPYRKPLKDGAPGH
                     GCGHNLLGVAGLASVMAIKQAIDTGEVKGTIKYFGCPAEEGGAGKAFMANAGVFDGTD
                     ICLTWHPDTFNGTMYANFLANYRVTFKFRGKTAHAAADPFNGRSALDAVELMNVGVNY
                     LREHMIPDARVHYVTIHGGGVAPNVVPDYAASLYSVRAPRTDQLDALFERVKNIARGA
                     ALMTETEVEIEVISGMSNMLPNETVNEVLQSKLEDVGAPKFTEEEHAFARELSKSIPP
                     DSLETSAYVYGLDSKAVAALKHKVLFEDILPPFKSEVILPGSTDVGDVSWVVPTGQIV
                     TTCWTLGSPGHSWQIVAQGKMGIGQRGMLYAGKVMASAALEFMQNDSLREKARNEFKE
                     KRAAANYISPIPPGIKPPLNI"
     gene            complement(8941..10203)
                     /locus_tag="VFQ23_13615"
     CDS             complement(8941..10203)
                     /locus_tag="VFQ23_13615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9907684.1"
                     /translation="MTPTYDKISLLRLAFKGLDDSELQEMADLTQLRVYPPEHVLCQE
                     GAYEDVFYIVAEGSLVITKKMLDEPGERVLRVGGRGDLVGEMALIQNAPRAATVRTIT
                     ECTMLEMGKRDFESILSKSPRMAIDIIRITLDRMRANDHMAIADLQRTNNILRQLDRN
                     KMDFIQVVAHELRTPLTVLNGYIKILRSSPDIKANESLGMMIEGIIKGTDRIHEVVNL
                     MLDVTRIDTETLRVVAEPVPLQQLIQELVQNMAPAMEGRKLEIIIDYGAETPQMFGDP
                     TLIVKALYQLVINAIKYTPDGGKVTISTSPCKIEDEIPGVQIIVSDTGIGIDAEHHEL
                     VFEKFYQVGSVTIHSSGKTTFKGGGSGLGLAIVRGVARAHGGKAWVESSGHNETNFPG
                     STFYLQLPIHPINPVGPSSTTTLRTSRL"
     gene            10202..10612
                     /locus_tag="VFQ23_13620"
     CDS             10202..10612
                     /locus_tag="VFQ23_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907685.1"
                     /translation="MINTRSITTAMAYNEKSNHITMIKNTNNPQKLASWCFLNLGDAI
                     MAAGPIAEIEEAFLQAFNKAGKPSTMAVFTRAESEGRLHCEVMAYFTPAAQGVARAFD
                     AQPCTKPARAGLELVSGDPECWSVLFHEKDKVHS"
     gene            10641..11192
                     /locus_tag="VFQ23_13625"
     CDS             10641..11192
                     /locus_tag="VFQ23_13625"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9907686.1"
                     /translation="MTKIFLLRHATPDSNFKHIPYFIHPGPPLSSKGEQEAEALAEFL
                     DAQGVVKLYHSPFERSSKTARIVSMQNDIPGIEDLRLTEWRSEDEKAEKVSERMSLAF
                     EEVSRESAVIGPIGLVSHGGPIATLLAKLGMEKEKLSEFRKKFDGSNPMPPAGVWAVE
                     WDDDNQLWNLDLIFVPSIREEAQ"
     gene            11254..11646
                     /locus_tag="VFQ23_13630"
     CDS             11254..11646
                     /locus_tag="VFQ23_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9907687.1"
                     /translation="MEITTKEFQHIELITIKGRLDSVVASQLAEVLDAAHHRGKYKIV
                     IDMSQLEYMSSAGFRALGDAQRNGKRLNRGEVVLAHVPDNIREALELVGFTEYFKILN
                     DVSSALEFAENLPTNDSTTSSPLASPKN"
     gene            12277..>12736
                     /locus_tag="VFQ23_13635"
     CDS             12277..>12736
                     /locus_tag="VFQ23_13635"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET9907688.1"
                     /translation="MKFTLNQTGPRLAAAVLGYIVLLIFLLTWNPFYLALPDEVNFSL
                     QIGPRDAIRNIILFLPVGFLYRLMQGRISTAILLGFVISGVAEVVQLFMPARTPSFID
                     LATNTLGAWLGAVLHDLVASRIAMSPRMVGRLALEVPLMGLLYLLVPLLWV"
BASE COUNT         3342 a         3017 c         3322 g         3055 t
ORIGIN      
        1 ggacggcatc gagatgcaga ccggtatgga gggtggtcta ttctccggtt tcaagcgggc
       61 ggtcaccggc gaaagtttct ttatcacaac cttcatgaac aacgatcagc gcagacggca
      121 cgtatcgttt gcggcgccgt atccgggcaa aatcatagcg tttgacctgg accagtttaa
      181 tgggaaggtt ctttgccaaa aagattcctt tctgtgtgcc gcgcgcggga ccgaaatcga
      241 gatcgccttc acaaaacgcc tgggcgccgg gttcttcggc ggtgaagggt ttatcctgca
      301 gaaattgact gggaatggaa tggtttttgt ccatgcgggc ggaacgatca ttgagaagga
      361 acttggtgcc ggcgaaactc tgcgcgtgga caccggctgt atcgtggcat ttgccaccac
      421 tgtggactat gacattcagt tcgttggcgg gttcaaaaat gcactctttg gcggcgaagg
      481 gttgttcctt gccaaattga ccggaccagg caaggtctat ttgcagagcc tgcctttctc
      541 gcgcctcgtg gaccgcatca agcgagggat gacgttcacc cagtctggcg gtgaacagag
      601 aggcgtagcg ggcatcggtg gcgatattat caaaaatatc atcagcggca gttgagaaat
      661 ccccactcga caaacgagtc tttgacggcc atgagaaagc ttgtcatctg actcgtttta
      721 atttaaactg aaagcgttcg cctgaaaagg ggctttttct gcatacttct tattgaatga
      781 ttgagattgg gtgggtatcg gttcacatgt cttggaatct aacgatcaat aacgggtaag
      841 gaggcaagat gaccacgatt aaagtcctgg gaaatcgcaa tgaaagcctg aagcaagtca
      901 gcttcgggaa agaacttgaa ggctttgaag tttcagatgc cattcaattc tcgaccgacc
      961 gctcagtctc cgcgtaccag gttattgaca aattaaaagc aaacgatctg atcgagttga
     1021 cctttgaagg cgatatccat cgctgggtca cagtcgagga actcgaacgg gattacaaaa
     1081 accagttatc acgcgggagc gagcaaggtg ttctggagat cccagcgttg cttcccacag
     1141 gcgatgcatc tcgtggggca accgcgctgg cgcttaagat cctgcgcgtt attaaattcg
     1201 atccggtcga agaaggggcg aaaaaattcg ccgagttctg ggataaaaga attatgcgcg
     1261 aaccgggctt atatcggttc gacaggggcc ttgataatag taaaaagagt ggaaaaatcg
     1321 aacacctcaa gatgaaacag gggaaaccaa tcctattatt cattcatggt actttttcag
     1381 aaactgcaag tgggtttggc ggcctaacgc ccgaggtatg gacgctcctg caggagcatt
     1441 ttggcagtga gatctatggc tacgatcatt acactttatc caaaagcccg atcgagaatg
     1501 ccctcgatct agttaagaaa ctcccgccga atgtaaaact tcacattgtc acccactcgc
     1561 gcggcggact aatcggcgaa ttgctctgcc gtagtggctg gaagggcaag cacggtccct
     1621 tcgatgatat agaccggaag ttgatggggg aaaacgcgaa aatggcaagc gaccttgatg
     1681 aattatcggc tgtgttgaag acgaaaaaaa tcagcgtgga acggtttgtc cgtgtggctt
     1741 gcccgtcgcg tgggaccaca ctggcttcca agcaggtcga tcgctggctg gaaatcctta
     1801 tcaatgtgtt tggaaagtta gttccagccg ggacgccctg ggcaatggga tacggggtgc
     1861 ttacagacct gctgctcgat ttcaaaaaac aggggatgaa cccggaggcg atccccggaa
     1921 tcgccgtcat gaacccggaa tcgaacttta tcaagatgat taaccgactg gacgtggagc
     1981 tcgatgttga cctgagcgtg atcgcgggcg atgttgagaa aaccggagtg atcggccagt
     2041 tggcaacatt tttcaccgat cgtttctact caggcgatca cgatctggca gtgcagacga
     2101 ggcagatgta tggaggcccc acgcgtaaga atgggcgcta tttctttcat aagggcccgg
     2161 gagtctggca ctgcagttat ttctccctaa aacggacggc agagatcatc aaggacgctc
     2221 tgacactcga gccgaaaaaa ctggaagata ggggtttcag gcctctcgag gaagcgtatg
     2281 cacgcgccgt gccagaattc caagtccaat tgacgtcgcg ctcttttcaa aaacgctcca
     2341 atctgcctca gcctgtagta tatgttcttc caggtgtgat gggcacacat cttgccgaac
     2401 gaggagatcg agtctggctg gacgtggctg acatggcaca ggggaaaatg gtaaatctga
     2461 atatcaacag taaaagcgtc gaggctgagt ccctggttca tcttgcctat ggaaacctgg
     2521 tggagtatct aagtgcaacg catgaagtga tccctttccc atacgattgg cggaagtcaa
     2581 ttctggatga agggaatcgt tttggggaag cgctggaagc aaagctaaag accttgaagg
     2641 aaacgaagtc tgatcagccc attcgcatcc ttgctcattc gatgggcggt ctcgtcactc
     2701 gggctatgat cgcgctcaga ccgaaactgt ggcaggagat ctgcaaacat aacggtgccc
     2761 gttttgttat gttgggaact ccaaataaag gttcgtacat gatcccgcgc ttaatcctgg
     2821 gacgggaacg aatattccgc atgttggcca tgctggatat caaaaattcc tcagaacaac
     2881 tgttggaatt aattgtacgt ttccctggca tcctccaatt attacctatg gacgatccga
     2941 aatggaattt caccgaagcc aaaacctggg acatatttcc gaaccttgcc aaacacaaat
     3001 gggtaaaacc tcgcgaggag gatctcaaac acgcaagaga tttccacgct ctactggcaa
     3061 aggaagagaa taaattcaag gaggatgatc cggttattta cgtggccgga tatgaccgcg
     3121 gagcacccgt agctctggat ttcgatgaga agaatgaatt tatcttccgg gggacgaatc
     3181 agggtgatgg ctcagttccc tggggatctg gcatattgcc ggaactggaa aaagataaaa
     3241 catattacat gcccgtgcct catggcgatt tgccgaatca tacagactcg tttgcggctc
     3301 tctatgattt gctgacagat ggaaggacca cgcgcctatc gaaaacaccg ccaagatatg
     3361 atcgcggtca ggaagactac tacccgctgc gtgatgaagg cgtggaaatc tatccgtccc
     3421 aactggattt ggaaaccacc gttcttcgaa tcacacacgc ggcccagacc gtgttatctg
     3481 caaaacctgt ccgcgtatct gtggtacatg gcaacctatc gttttctgaa catccggtgg
     3541 cggtcggcca ttatgaaggt gatagtttat atagcgcaga gaggcatctg gaccattatt
     3601 tggatggcaa actctcagct cgacaccaac tcggcctgta tccgggtcct gatgggacgg
     3661 cagaagtcgt attgaacgac aggggcaaaa agccaggcgg agcaatcatt gtagggctgg
     3721 gcaaggccgg tgagctttct ccacacaggt tgaccagttc ttttgcgaac gccttgcgcg
     3781 agttcggtgt caaatccgtt gaaaacgaga aagtaggcga ggacggcgaa ctgaagattt
     3841 ccactctgtt aattggaaca ggtggtatgg ggcttaccgt caccagttca atagacgcga
     3901 tcctcgcagg ggtcattcaa gccaacaaaa gcttcagtca gcttgctgaa acaggtcagg
     3961 gagaaaatca gcggttcaac aatattcgta ttgctgagat caaatttata gagctctata
     4021 aggaccaggc catcctggct atcaaagctc tcaacaatcc cctgctaaac agagatgggt
     4081 ttgacatcaa accgagattg gaaaaaatgc agggaggctg gcagcgtgcc gcctacgagg
     4141 aacccgccgg ttggtggcca cgcctttatg ttcgagcagg agaaaacgca gacagttcgt
     4201 tggtcttttc ggttccgacg gcgcgcgccc ggtcggagga atccctgctg gatatccaac
     4261 gacgaaattt tgatcggctc atcgcgcagg ctgtcaaaaa tcccaattgg gatcaggcgc
     4321 ttgccactgc catgttcgaa ctgatgattc ccaatcggct gaaaagttct ttcaaggatc
     4381 tgaccaacgt gcttttcgtg gtagacgaga aagctgcagg ctatccctgg gagttgctct
     4441 atgaccgccg gaccggtcag gagaagccgc tggtgattca gatggggatg atcaggcaat
     4501 ttagcaccgc cacgttccag gagcgcgtga tcgacgtcac gaataagaat atcatggtgg
     4561 tcggcaaccc cgccgatacg cctgataatt ttccgaacct gcccggcgcg gagcaggaag
     4621 ggagactggt cgccgaaaaa ttcaaggagt ttcaatacga cgtgcgcgcc gcaatctacg
     4681 ccgactccag caccattatg agcgacttgt tctcacagga ttatcgcgtc cttcatctgg
     4741 caggtcatgg ggtctttgaa tatgagtaca aagacaaaga agattccgaa ccggaagtat
     4801 ttaccgggat ggtgttggga aacggtgtgt tcctgactgc gaatgaaatt cagaacaaaa
     4861 tgcacatacc agagctggtc ttcattaatt gttgtcatct gggcaaaatc tcttcaagat
     4921 cgaagcagcc accagattat gcatacaatg aattcgcagc cagcctctcg agaaaactga
     4981 tcgatatggg cgtcaaggca gtcatcgccg ctggttgggc cgtggacgat gccgctgctc
     5041 ttactttcgc cgaagttttc tacgatcatc tgctgaaagg cgcgtccttc ggcgagtctg
     5101 tcaaatctgc acgacaggag acatatgatc tgcacaaaga ccgaaccaat acctggggag
     5161 cctatcaatg ctatggtgac cccgattatc gattcgctgc atcatcttcg ggcggtcaaa
     5221 gatcgaaaga aaaattcgtt gatgtcgagg aagtcatttt ggcgatcagg cgccagcatg
     5281 aaatttcaaa gactacagca gtgctcggac tcaaaaagat ccgtaatgat ttgagaaatc
     5341 tccagaagga gataaaagaa caaaatgagt cgtggctcga agatgcgctg gtggcggatg
     5401 catttggcgc tgcatttgcc gaagccttct gcttcgagga ggctattgag tcgtatgagc
     5461 tcgcaaccgg aagtggcaaa tccacggctg ccatcaaagc actcgaacaa acggtcaatt
     5521 gccgcatccg cctggcagta cagaatgttc aaaccgatcc gcggaactac ggtgagtcaa
     5581 taaagtccat caaagaacag attcgaaagc tcatccggct gatggatgca attggtgaaa
     5641 cgccggagcg gctgtcgatg gttggaggcg gatacaaaag gcttgcccgc ttgaccaggg
     5701 ataagtcgcc agatgaatcg gcttcagctc ttgataagat ggaaaagtat tatgcccgcg
     5761 cctgggattt taataaagag gaagtatatc cgctgacaaa tttagcgctt gcgaaaatca
     5821 tacgagctct gtggaatggc aagcccagtg caaaacaatt gtctgagata gaagcgcaga
     5881 ttaaggaagc ccggcggttg gctgtgatcg aacagaaaag atctccggat gacttttggt
     5941 ccgccatggg actcacggat ataaaaatgc tggagcatct cctaaaatac ctgaagggca
     6001 gccagcggag ttttagtgag aaagtccatg atgaactggt ggaggaatac aaaaaggctt
     6061 ggggtcaata tgggtctgca cgcgagatca attctgtcat tgaacactat gcctttctac
     6121 tggccgtcat caagaaacca ggctcacaga aagatgtaca taaagctctg cacaatgtgc
     6181 tggacaatat tctggattct ctcacagcaa cctacgaaaa agtggggtaa tcagacagga
     6241 tagtaagttt tagcgtatcg ggagatatgt gtaatggaga aagtctggca agttatccgt
     6301 gccattgggc tgccaatcgt tgtactctta agtggtgcaa tggtgatgtc gattctcagc
     6361 gggcatggtt acgtcatgga acaactgcga gaaaacgcag attcaaagga ccgaaaacct
     6421 ctcaacatgc gcattctcgg atacgatacg aacgacgtag cccggcactg ggctgcgctt
     6481 gacgaaaggg ctctgcaaag cgaacgccga tttttggaat tggatctgat ctttccgact
     6541 ttttacggtg cagcactggc ttttgcatta tggcaggcct ggagggtcac cggatttgtc
     6601 ttctcccctg cctgggtaat agtccctgtt gccatcacca taatcgcaga ttggacggag
     6661 aacctcattc atttatctca actgcaccgt tatatagaga gtggcgaggc tggcttacag
     6721 actgcatcta ttcaaattgc aagtggggca acgattgtca agcttgcatt ctttgcgggt
     6781 atattgctgt ttagcgcctg gaagtcggtc cagaattttc agagttcgtc agttcgtcgc
     6841 ccgtaatgcc ggccattttg agtaatcact ggaagagtgt ggacctgctt cacttttcgt
     6901 cctcaaacga ctcgtcaaag agatctttta cgccaaagac gttctcataa aggctcaaca
     6961 attcgcgcgc tttcaggcga aaaccttcat cagaattttc ttccaattgg gcaaccaatc
     7021 gttcccgaaa attagggttg ggtgaaactg gcagattcat ctctagttca tggatcgacg
     7081 cttttgcttg tccaattact gcaagtgcat ctggaagtga agttgtttca tcaagcaatt
     7141 gtttcagcaa tttacactta taggtgcggc aaaactgggg atagtgagga gatgtataaa
     7201 tcgtacatac gccctgccag agcgggcacg gctggctaaa gcctcgctgg ctgggcacag
     7261 atcgaaacac attcaaccca agcgactcag ccgagtccaa ctcggatgcc ttaagctttg
     7321 accagataaa taaatgcccg gtgcagcaca acccacagga cttgcataga atatttgctt
     7381 gagattgatt tgtttcttca acagatgtca tgccaggata ataacatgga tgatggatgg
     7441 aaaccattga ctggtgggga tttttcttat cgacagaata taataaagca cagatccaaa
     7501 cctaaccaag gagaagacca tggacctttc tgagtggata gaatccaacc gttcaatgtt
     7561 cattgaattg agtaatgaag tctggggata tgccgaactg ggatacaagg aatttgaatc
     7621 ttccaaaaca ttggtggacg cgttcgaaga ggcaggcttt acgttggaac gcggcgtcgc
     7681 cgatattccc acggccttcg tcgccaccta tggaaacggc gagggacccg tgatcggcat
     7741 ccttggggaa ttcgatgctc tgcctggtct cagtcaggac tgcgtgccgt accgaaaacc
     7801 tttgaaagac ggcgctcccg gtcacggctg cggacacaac ctgcttgggg tcgccgggct
     7861 ggcatccgtg atggcgatca agcaagccat cgacacaggt gaggtaaaag gcacgatcaa
     7921 gtattttggc tgccctgctg aagaaggcgg cgcgggcaag gcttttatgg caaatgcagg
     7981 cgtgttcgac ggaaccgaca tttgcctgac atggcatcct gataccttca atggcaccat
     8041 gtatgccaac ttcctggcaa actatcgcgt cacctttaag ttccgcggca agaccgctca
     8101 cgcggccgca gatcccttca acggacgcag tgcgctggat gcggtcgagt tgatgaatgt
     8161 gggagtcaat tatttgcgcg aacatatgat ccccgatgcg cgcgtgcatt atgtgacgat
     8221 ccatggcggt ggagtcgcgc cgaatgttgt ccctgattac gccgcgtcgt tatacagcgt
     8281 ccgcgcgcct cgcaccgatc aactggatgc gctcttcgaa cgcgtcaaga acattgccag
     8341 gggtgccgcg ctgatgaccg aaacggaagt agagattgaa gtgatctcgg gcatgtctaa
     8401 tatgctgccc aatgaaactg taaatgaagt gcttcaatcg aaattggagg atgtgggtgc
     8461 gcctaaattt acggaagaag aacatgcttt tgcgcgcgag ctgtccaagt caattccgcc
     8521 agatagcctt gaaacgagtg catacgtcta tggtttggat tcaaaggccg tcgcggcgct
     8581 gaagcacaag gtcctgttcg aggacatttt gcctccgttc aaatctgaag tcatactccc
     8641 cggttcgacg gatgtcggcg atgtctcgtg ggttgttcct accggacaga tcgtcactac
     8701 ctgttggact ctgggttcgc caggtcattc ctggcaaatc gtagcacaag gcaaaatggg
     8761 cattggtcaa cgcggcatgt tatatgccgg caaagtaatg gcatcggctg cgctggaatt
     8821 catgcaaaac gatagtctac gcgaaaaagc acgaaatgaa ttcaaagaaa aacgtgcagc
     8881 tgctaattac atttccccga tcccacctgg gatcaaacca cccctcaaca tataagggac
     8941 ttacagtcgc gaggttctga gtgtggttgt gctcgagggt cccacggggt taattgggtg
     9001 gatgggtaat tgtagataaa atgtgctgcc agggaagttc gtttcattat gacccgagct
     9061 ctccacccag gctttgccac catgtgcccg cgctacaccg cgcacaatcg ccaatcctaa
     9121 ccctgagcca ccgcccttga acgtcgtttt gcccgaggaa tgtatggtga cgctgcccac
     9181 ctgatagaac ttttcaaaga caagttcatg atgctccgca tcgattccga tgcctgtgtc
     9241 actcacgata atttgcacac cggggatctc gtcttcaatt ttgcatgggc tggtgctgat
     9301 cgtcaccttg ccgccatcgg gagtgtattt gatggcgttg attacaagtt gatatagtgc
     9361 cttgacgatc agtgttggat cgccaaacat ctgtggcgtc tcggcgccat agtcaatgat
     9421 gatttcgagt ttgcgccctt ccatagccgg cgccatattt tgcactaact cctgtatcaa
     9481 ctgctgcagc ggcacgggtt cggcgaccac cctcaaggtc tcggtatcga tgcgtgtcac
     9541 atccagcatc aaattaacca cctcatgaat ccgatctgtg cccttgatga tgccctcaat
     9601 catcatgccc agggactcat tcgctttaat atcaggagac gaacgcaaga tcttgatgta
     9661 tccgttcaag actgtgaggg gcgtgcggag ttcatgagca accacctgga taaaatccat
     9721 cttgttgcga tccaactgtc gtaggatgtt gttggtgcgc tgcaagtcgg cgatggccat
     9781 atggtcattg gcacgcatgc gatccagcgt gatccggatg atatctatcg ccatccgtgg
     9841 acttttactc aggatcgact cgaaatccct tttccccatc tccagcatgg tgcattcggt
     9901 aatcgtccgc acggttgctg ctctgggggc attctggatc aatgccattt cacccactaa
     9961 gtcaccccga cctcctacac gtaagactct ctcgccgggc tcatccagca ttttcttggt
    10021 gattaccagg cttccctccg cgacgatata aaatacatct tcgtatgcgc cttcctggca
    10081 caaaacatgc tccggcgggt agacgcgaag ttgagttaga tctgccatct cttgcaattc
    10141 gctatcatcc agccctttga atgcaaggcg cagaagagaa atcttatcgt aggtaggagt
    10201 catgatcaac acccgtagta taacgacagc aatggcgtat aatgagaaaa gtaaccatat
    10261 tacaatgatt aagaacacaa ataaccccca aaagttggca tcctggtgct tccttaatct
    10321 cggtgatgca atcatggctg ccggcccaat cgccgaaatt gaagaagcct ttctgcaagc
    10381 attcaacaag gctgggaagc catccactat ggctgtcttt acgcgcgctg aatcagaagg
    10441 tcggcttcat tgtgaagtga tggcttattt cacaccagct gcacagggag tagcacgggc
    10501 tttcgatgca caaccctgta caaaaccagc gcgagcaggg ttagagttag tgtctggtga
    10561 cccggaatgt tggtcagttt tatttcacga aaaagataaa gtacattctt aaagggagat
    10621 ttatcaataa ttcatccaat atgacaaaga tttttctcct aagacatgcc accccagatt
    10681 cgaacttcaa gcatatcccc tattttattc acccgggacc tcccctgtca tcgaagggag
    10741 aacaggaagc agaagcgctc gcagaatttc tggatgcgca aggtgttgtg aaactgtacc
    10801 atagcccctt tgagcgtagt agcaaaaccg cgcgcattgt ttccatgcaa aatgacatcc
    10861 cgggcatcga agacctccga ttaactgaat ggcgatctga agatgaaaaa gcagagaagg
    10921 tcagtgaacg aatgtcgctt gcgtttgagg aagtctcccg ggaaagcgcg gtcattggac
    10981 ccattggact ggtatcccac ggaggtccga tcgctacttt actagcgaag ctgggcatgg
    11041 aaaaggaaaa actatcagaa tttcggaaaa aattcgacgg gtccaacccg atgcctccgg
    11101 caggggtgtg ggcggtagaa tgggacgatg ataaccagct ctggaatctg gatttgatct
    11161 ttgtcccttc aatacgtgaa gaagctcagt gaaagtttag cattgagccc ttgtatcttc
    11221 gaacaatcag tctacaagtt tggaggataa gccatggaaa tcacaaccaa agagttccag
    11281 catattgaac tgattacgat caaaggtcgc ctggatagcg ttgtagcttc acaactggcc
    11341 gaggtgcttg acgctgctca ccatcgtggc aagtataaga tcgtgatcga catgagccaa
    11401 ttggaataca tgtccagtgc gggtttccgt gctctcggag acgctcaacg taatggcaag
    11461 cgtctgaacc gcggtgaagt ggtgctggcc catgtgccgg acaatattcg tgaggccctt
    11521 gagcttgtcg gattcactga atatttcaag attctgaatg acgtctcttc tgctctggaa
    11581 tttgcagaga atttgccaac gaacgattct acaacgagtt cgccactggc atctcctaaa
    11641 aactaaagta taaaactctc cagcttgagt tgaatcttgc gatcaagttt caagctggtt
    11701 tgatttcctc aatcgcatac gggcatcgat ctacaggaga ttccaatgaa agttctcgcc
    11761 aagagcgcaa ggggtcagag ctaacccatc gattcgcgtg cagcggattg aatgggcaga
    11821 taagttgcta acagagtgaa ggaaggaatt gtcagatcgc tcgttctgcg ctatccttat
    11881 tatgttgtcc aagacggata ccaatgcttg aacaagaaag gagaaaaatc atggctggaa
    11941 atggaaaaaa agataaaggc aacaaggaaa aaaagaaaaa gccgaagctt aatattaagg
    12001 agaagcgaaa acagaagaat gaaaagaaaa ccaagtaatt gagatcttta aagaccgacc
    12061 tgcccaatca ggtcggtcaa cgagttcaaa gactagcttg gaataattat ggggataagg
    12121 ttgaggacga tgattcccaa aagaagtacc cacagcaatg aaccacgatg ttttatcctt
    12181 cttgcagcag agtaatttcg cacgtgcgaa gttctagccg cttcagcttg cctgatcaaa
    12241 gctatcgacc tcctgtaaat atctatattc cataaaatga agttcacctt aaatcaaacc
    12301 ggaccacggc tggccgcagc agtgcttgga tatattgtcc ttctgatatt tcttctcacc
    12361 tggaatccgt tttatcttgc cctgccagac gaggtgaact tttcgcttca aatcggaccc
    12421 cgtgatgcga ttcgaaacat tatcttattt ctgccggttg gctttctcta tcgtctgatg
    12481 cagggcagaa tctcaaccgc aatccttcta gggtttgtga ttagcggtgt cgccgaagta
    12541 gttcagcttt ttatgccagc tcgcacaccc tcctttatcg atctggccac aaatacatta
    12601 ggtgcctggt tgggggctgt gttacatgac ctggttgcga gtcggatcgc gatgtcacca
    12661 cggatggtcg gccggctggc gctggaagtc ccattgatgg ggcttctgta tttactcgtg
    12721 cctttgcttt gggtca
//
LOCUS       DASTJP010000469         3856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1280728, whole genome shotgun sequence.
ACCESSION   DASTJP010000469 DASTJP010000000
VERSION     DASTJP010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280728"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..2989
                     /locus_tag="VFQ23_13640"
     CDS             452..2989
                     /locus_tag="VFQ23_13640"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9907689.1"
                     /translation="MPIPKTKLRPPTLRQPMVPRPRLTSSFTDRRPLTVVSAPAGSGK
                     TTLALEWLASKKGKVAWLSLDSDDNDPIRFINGFSAALHAAGISFKAPSGQRNLKTII
                     TELINQLEDVDVEAVTLVLDDYHLITEESIHSTIAYLLDHIPASLQLVIVTREELPFS
                     VARLRARGQLREFRIDELRFNLEETKLFLNKVMGLNLSGEQIQSLEAHTRGWIAGLQM
                     AGISIQSNTDLAVSDANERQFIAEYLLTEVFNHQSKEVQGFLLNTSLLDRFSAPLCKV
                     IYSGNANKMLSHLEKSNLFISVVDAWYQYHPLFREFLQAQLQKKHHERIQTLRRKVSE
                     WLEANGFIAEAIPHTFAISDHEAAARLIAVLAPDYFRRGELVTLRRWLDQIPESVIWN
                     NPRLCLTQIWLLLDSNRQNDAQTYFDRLGHFFEKNLRGEFLAVRALHAAMTYQPELAL
                     KFAQQAQKTPEAKDPFIQTYVSFGLGAAQKMGLHFFQAEGSFRQALALADSADNSYIA
                     ISSLANLADVLYLQARLDEAEKVCQDALQRFQETIPEASDWYWTLARIAYQRNQLDEA
                     FKFINISIDLCADSQEIHLHARALTQRAIIEFAIGDKDAAYIDLDSADGLARGLQNKT
                     SLRLVIRQKIIFAVENGDNASALQWLKRLAEFGEQPFPFFYAFAKGRVLFAEKNFKEA
                     NAAFESALKSLNEVDYALFRIEILAWHAICLNKLGQTNESAQVLRNAIKAAQAGRVIR
                     PFVEVYNHAPALMEQIGRGEFAWVLDEILPNGQQVQVEAGYESEDSDLTRREKEILNL
                     IELGMSNREMAERLVIAEGTLKRHVANIYQKLGVHNRTQAIKQFYRQ"
     gene            3227..>3856
                     /locus_tag="VFQ23_13645"
     CDS             3227..>3856
                     /locus_tag="VFQ23_13645"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907690.1"
                     /translation="MSKTIRSRDEIPAKYKWNAASVYPSDEAWDSAADALLAKLEEGK
                     KLVGKVGKSAKKVADALDLSSAIQEELGKLATYAVISQAVDNADQSAARIYGKIQSVY
                     GQAFAGLSFIEPEILAIDRKQLNKWRKEEKRLKPYAQYFDNLLRQQAHVRSAEVEEVF
                     GLALDPLGTLSTNMNLMTNSEMHYPNAQTSDGKELTVTQGSLDEILASPD"
BASE COUNT         1022 a          959 c          941 g          934 t
ORIGIN      
        1 ccgcagtggg ttgttggaca ggcaaagacg taggggattg agggctacaa gacaaattca
       61 tagccaagac aagtaccgta cagtgatata agatgtgcct tttattcatt cttcctcctt
      121 tgcgtttagg gagcattgca ctactacctt taacgactat tttggtgatt ttccgccaca
      181 aatcaggttt cggcctacga aatcgtggtc aattattctg accttactat agcaaatttc
      241 ccatgccaac gggtcacgaa aactgttaca agaagtgtga ctattttagt acttaaagtt
      301 ccaaacgtca gatcaaaagg ggaccgtttt ctgaatagat gaggcaaggc aatagaagtc
      361 gtgtttgggc gcgaaatcag aacagagtag agtagttccc gaggatgggt atgaattttt
      421 catttaatga taaactccca aagcactgct tatgcctatc cccaagacca aactccgccc
      481 gcccacgttg cgtcagccga tggtgccgcg tccgcgcctg acgagctctt tcactgacag
      541 acgcccgcta acggtcgtct ccgcgcccgc tggttccggc aagacaaccc tcgcgcttga
      601 gtggttggcg tctaaaaagg gcaaggttgc ctggctgtca ttagactcag atgataacga
      661 cccgatccgt tttataaacg gtttcagcgc cgcacttcat gccgccggta tatccttcaa
      721 agcgccttcg gggcagcgca acctcaaaac catcattacc gaactcatca atcagctgga
      781 agatgtcgac gttgaagcgg ttacgcttgt gctggatgat tatcatctca tcactgagga
      841 atcaattcat tcgacaatcg catatcttct tgaccatatc cctgcgtcgc ttcaactggt
      901 gatcgtcacc cgcgaggaac ttcccttctc tgttgcgcga ttacgcgctc gcggacaatt
      961 gcgcgaattc cgtattgatg aactgcgttt caatctcgaa gagacaaaac tattcctgaa
     1021 taaggttatg gggttgaatt tatctggcga gcaaattcaa tctctcgaag cacatacgcg
     1081 gggatggatt gccggcttac agatggcggg catttcgatt caatccaaca cagacctggc
     1141 cgtttctgac gcgaacgaac gtcaattcat tgcggaatat ctgctcaccg aagtcttcaa
     1201 ccaccagtcc aaagaggtgc agggcttctt gttgaataca tcccttctgg atcgattctc
     1261 cgccccacta tgcaaagtta tttactcggg caatgcaaac aagatgctat cccatctcga
     1321 aaaatcaaat ttattcatca gcgttgtgga tgcctggtat caataccatc ccctctttcg
     1381 tgaattcctg caggcacagt tgcaaaagaa acaccatgag cggatccaga ctttgcgtcg
     1441 caaggtctcg gagtggctcg aagcaaacgg attcatcgcc gaagcgattc ctcatacctt
     1501 cgcgatttcg gaccatgaag ctgccgccag gttgatagca gtattagcgc cagattattt
     1561 tcgacgcggc gaacttgtca cgctgcggcg ctggctggac cagatacctg aatcggtaat
     1621 ctggaacaat ccgcgcctct gcctgaccca gatctggctt ttgctggatt ccaaccgcca
     1681 aaacgacgcg cagacgtact ttgaccggct cggtcatttc ttcgagaaaa acctgcgcgg
     1741 tgaatttttg gcagtccgcg cgcttcatgc agccatgacg tatcaaccag agctcgcctt
     1801 gaaatttgca caacaggcgc agaagactcc cgaagcgaaa gatccattta ttcaaaccta
     1861 tgtttcattt ggattggggg ccgcgcaaaa gatgggactg cacttcttcc aggcagaagg
     1921 cagttttcgt caggcattgg ctttagccga ctcggcagat aattcttaca tcgccatttc
     1981 ttcgctggcg aatctcgctg atgttttgta cttgcaggcg aggcttgatg aagcggaaaa
     2041 agtctgccag gacgcacttc aacgttttca ggaaactatc cccgaagcca gcgattggta
     2101 ttggacactt gcacgcatcg cctaccagcg caaccaactc gatgaagctt tcaagttcat
     2161 taacatctca atagatctat gtgccgattc acaagagatt catcttcatg cgcgggcact
     2221 tacgcaacgt gcaatcattg aatttgcgat tggtgataaa gacgccgcgt atatcgattt
     2281 agattccgca gacgggttgg cgcgtggctt gcagaacaaa acttctctga ggctagtgat
     2341 acgacagaaa ataatattcg cagttgaaaa tggagacaac gcctctgcac tacaatggtt
     2401 aaaaagactc gccgaatttg gtgaacaacc cttccccttc ttttatgcat ttgccaaagg
     2461 acgagtctta ttcgcagaaa agaatttcaa ggaagccaat gcggcctttg agtctgcatt
     2521 gaagagtctc aatgaagtgg attacgcgct gtttcgcatt gaaatcttag cctggcacgc
     2581 catttgtttg aacaagcttg gtcaaacaaa cgagtccgcc caagtgttga gaaatgccat
     2641 caaagctgcg caggcgggca gggtgatccg tccttttgtg gaagtctaca accatgctcc
     2701 agcgctgatg gaacaaatcg ggcgcggcga atttgcctgg gtgttggatg agatccttcc
     2761 aaacggtcag caggttcagg tcgaggcggg atatgaatct gaagattccg atttgactcg
     2821 gcgcgagaag gaaatcctga atctgatcga actggggatg tcgaaccgtg agatggcaga
     2881 aaggcttgtc atcgcggagg ggaccttgaa acggcacgtc gccaacatct atcaaaagtt
     2941 gggggtgcac aaccgcacac aggcgattaa acaattctat cgacaataga atcttgagac
     3001 cctaaaggat tcgtggcaag caaagacaag attcaactag tggatcctgg caatttagtc
     3061 cacgattgct cggaaatcct ttagggtctc tttcaataaa ttaaaaatac atacaaaatt
     3121 acatatttcg gctcatgccc aaatagcgtt ccgctgttaa agtaaaggtg acggtcaatt
     3181 cccgcgaagt gattgactgt cacctttcaa cttaaacgga ggagctatgt ccaaaacgat
     3241 tcgctcacgg gatgaaatcc ccgccaaata taaatggaac gcggcaagtg tttatccatc
     3301 tgacgaagcc tgggatagcg cagctgacgc gttgcttgct aaactggaag aaggaaaaaa
     3361 gctggttggt aaggtcggga aaagtgcaaa gaaggtggcc gatgcgctag acctgagttc
     3421 tgccattcag gaggaactcg gcaaacttgc cacctatgca gtcatctcac aggccgtgga
     3481 caatgctgac cagtctgccg cgcgcatcta tggaaaaatc cagagcgtgt acggacaggc
     3541 tttcgcgggg ttgtcgttta tcgaaccgga aatcctggcg atcgatcgca aacagctcaa
     3601 caagtggcgc aaagaggaaa aacgattgaa gccctatgcc caatatttcg ataacctgtt
     3661 gcgccagcag gctcatgttc ggtctgcgga ggtggaagag gtattcggtc tcgcgctgga
     3721 tccgctcggc actctctcca ccaatatgaa tttgatgacc aacagcgaga tgcactatcc
     3781 caacgcgcag acctctgacg gaaaagagtt aaccgtcacg cagggctcac tcgatgagat
     3841 cttggcctcg cccgac
//
LOCUS       DASTJP010000470         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_699136, whole genome shotgun sequence.
ACCESSION   DASTJP010000470 DASTJP010000000
VERSION     DASTJP010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699136"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFQ23_13650"
     CDS             <1..201
                     /locus_tag="VFQ23_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907691.1"
                     /translation="AAKRFGLAVAETNLQNILYQGSVFATIIVLEFHWLQDEDDCKKL
                     RESSFQEVNIKTFIEQVLLGRL"
     regulatory      182..285
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            488..1306
                     /locus_tag="VFQ23_13655"
     CDS             488..1306
                     /locus_tag="VFQ23_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907692.1"
                     /translation="MKTHTLNIGSIFILLALVFVPLSGRVHAQEPALELELLTKFETS
                     ESLGFSPDDVTYSPPHAHLFITSNGPKKTGIFEVTLQGELVRHIPFFQTPVGSCCAGF
                     SITRAHSGPKVGHFFLADLIDQTTLVHEFDSNWNWINAFPVTASYPDSHPGDGIAFNT
                     ISKNIVVAEEVHGELIEVTTGGQFLRTFPAVNVQGITYNEPTGTYFGVHVSRWLREIS
                     SDGIILRQFDLTPYGIQQPVGVASGQGKLFIADELDVANTGGYIYIFRSPKKEK"
     gene            complement(1631..2317)
                     /locus_tag="VFQ23_13660"
     CDS             complement(1631..2317)
                     /locus_tag="VFQ23_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907693.1"
                     /translation="MGNTCRQGIIALEYLEEGKAKAARAKKAVEVLQKYTAGFQQIWD
                     EKQQVEARRTAYSEKLEKINSFRSQLEKRNKEYCQLAISSNSQERGYSLEAILKQLFD
                     LFDLDPKASFKIEGKQLDGAFTFDGADFLFEAKWQTELVRANALDSLASKVQRKLDNT
                     LGLFLSVNGFSPDGIAAHSTGRKVVLLMDGSDVMAVLDERIGFPELLKRKRRHAAQTG
                     EIYLRYQDML"
     gene            complement(2761..>3251)
                     /locus_tag="VFQ23_13665"
     CDS             complement(2761..>3251)
                     /locus_tag="VFQ23_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9907694.1"
                     /translation="PVTYLMGANTYRLMSSFAGGEPPPGTAEMEAEEEASVDELTRTP
                     KVVFSASLEEPLTWANTTLVRGDAVQAVRAMKEEGSGILSTLGSVSLCRSLLKAGLVD
                     RFRVVVFPVITGATGAERIYDGYPDVALDMVSTRTFDGRIQLLEGVPRVLAGPPGVKN
                     DP"
BASE COUNT          766 a          798 c          818 g          869 t
ORIGIN      
        1 gccgccaaac gatttgggtt agctgtggct gaaaccaatc tgcagaacat cttgtatcaa
       61 gggtctgttt ttgctacaat aattgtgttg gaatttcact ggctccaaga tgaggatgat
      121 tgtaagaaac taagagagtc gtctttccaa gaagtgaaca tcaagacctt cattgaacag
      181 gttttattgg ggaggctatg aaccgctgat ctaatttggt cgcttaatca tcgcaagatg
      241 ggcaaggtca gcggagagcc aatagcgaaa ccggtcacca tccggtttcg tttcttttta
      301 aggcgatttg ccctgttggg gtggtgtccc ggcgggatgt ttgatgggag caagtagcgc
      361 tctgtgtgaa tttacacaag atctaccagt acttgggtga gccccatgct ttttgaaagg
      421 aggtgtcaaa agaatataga atttttgagt gttccgtcgt tttcagcata tagaaaagga
      481 gaacgaaatg aaaacgcata ccttgaacat tggctcgatc ttcatcctgc tggcgctggt
      541 tttcgtgccg ctttcgggga gagtccatgc ccaagaaccc gctctcgaat tggaattgct
      601 gacgaagttt gaaacctctg agtctctggg tttcagccct gacgatgtga cctatagccc
      661 acctcatgct cacttgttca taacatctaa cggtcctaag aaaactggaa tcttcgaggt
      721 gactctgcaa ggagaactcg taaggcatat tccgtttttt caaactccag ttggcagttg
      781 ctgtgcagga ttcagcataa ctcgagcgca cagtggacca aaagttggtc acttttttct
      841 agcagacctc atagatcaaa caactctcgt tcatgagttc gactcgaatt ggaactggat
      901 caacgctttt cctgttacag cgtcttatcc ggattcgcac cccggtgatg gaattgcttt
      961 taacaccatc agtaagaata ttgttgtcgc cgaggaggtt catggggaac tcatagaggt
     1021 tacgacaggt ggacaattcc taagaacatt tcctgcggtt aatgtgcagg ggatcaccta
     1081 taatgagcct accggcacct attttggagt ccatgtttca agatggctgc gggagatttc
     1141 aagtgatggc ataatacttc ggcagtttga cctaactcct tacggaattc agcagcctgt
     1201 tggcgtagca tcgggtcagg ggaagctgtt tattgctgac gagttggatg tggccaacac
     1261 tggcggctac atctatattt tcagatctcc aaagaaggaa aagtagccct catttcatat
     1321 tgtgcaagag tacagtgcag atgctgcggg gttggatctg cagctcttcg aagctaagat
     1381 gggtcatgca tccagcttcg aaaacgggat tttactcaag agttgtgctt gtcagaaaat
     1441 cacggttcaa tcagataaac cgctgggcat aaatcttctc aagcagagaa atgcccaacg
     1501 gctcgcgttg gtggcaggtg ggagcgtgga ttcgctgtcg aaacgggagt ccgcgcaagc
     1561 caggaacatg ctcaaaaacg cggcgcgtac cccaagtcgg gtgcacgctg tgttgggtgc
     1621 gtttttgttt tcaaagcata tcttgatacc tgagatatat ttcgccagtc tgggcagcat
     1681 gccgccgttt tctttttaag agttctggaa agcctattcg ctcatctaat accgccatta
     1741 catcgcttcc gtccatcaac aatactactt ttcgccccgt agaatgggcg gcgattccat
     1801 caggcgaaaa gccatttact gataagaata aaccaagggt attatccaat tttctttgca
     1861 cttttgatgc aagggaatca agcgcgttgg cacgaactaa ttcagtttgc cattttgctt
     1921 caaacagaaa atctgcgcca tcaaatgtaa acgctccgtc tagttgcttg ccttcgattt
     1981 tgaaagatgc ttttgggtca aggtcaaaca gatcaaagag ttgtttcaat attgcttcaa
     2041 gactataacc acgttcttgc gagttgctag atattgcaag ttggcaatat tctttgttcc
     2101 gtttttctaa ctggctcctg aatgagttaa ttttctccaa cttttcagaa taggcagtgc
     2161 gtcttgcttc tacctgctgt ttttcatccc aaatttgctg aaatcccgca gtgtattttt
     2221 gcaagacttc tacggctttt ttcgcccgcg cagctttcgc tttcccctct tctaaatatt
     2281 caagggcgat tataccctga cgacacgtat tccccaaaag ttgtccctat atacgtgcaa
     2341 tgagaggata atcaccaatt attttcacca actgcgttca ataagagttc ttgcgatgat
     2401 tgttctattc gagcgggcgg ggcaagccgc ccctacgcgt cagatcatat gcttcgtagg
     2461 ggttgcggac tgcccgacct gcattaacag ttcaatcagt ctgaaatacg atgtaagtca
     2521 ccttacgcag atgaatatct gtggagagga caggacaatt tcccgatgat gcgcatcagt
     2581 cgcgcgacat agccatacag gttggcgagc cgacctgagc catgctgctc tgggctttgt
     2641 ccataatcgg gcagactttc agcacctata tcacggtgcg tactccgggc gtccgttggg
     2701 ccgatagact tggagcgttt tgcccttcgg tagcctacag tgtgttaatg ttccctgact
     2761 tcagggatcg tttttcacgc ccggcggacc ggcaagcact ctggggacgc cctccagcag
     2821 ctgaatcctg ccgtcgaacg ttcgggtgct aaccatgtcg agcgcgacat cgggataacc
     2881 gtcgtagatg cgttccgcgc cggtggcacc ggtgatcacg ggaaagacga ccactcggaa
     2941 acggtctaca agacccgcct tgagcagcga ccggcacagg ctgacgctgc cgagcgtgct
     3001 gaggatgccg gatccctcct ccttcatcgc ccggaccgcc tgcacggcat cgccgcgcac
     3061 gagggtcgtg ttggcccacg tgaggggctc ctcgagcgaa gccgagaaca ccaccttcgg
     3121 cgttcgtgta agctcgtcga cgcttgcctc ctcctcagcc tccatctcgg cggttcccgg
     3181 cggcggctct ccgccggcga agctggacat cagccggtac gtgttcgcgc ccatcaggta
     3241 cgtcaccggt g
//
LOCUS       DASTJP010000471         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1175352, whole genome shotgun sequence.
ACCESSION   DASTJP010000471 DASTJP010000000
VERSION     DASTJP010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175352"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFQ23_13670"
     CDS             complement(<1..793)
                     /locus_tag="VFQ23_13670"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9907695.1"
                     /translation="MSEKGFLGKVGMLFVALSLLMSFGATTFANAQEAGPEMLDPQLG
                     VRTVVSGLNLPTTMAFIGPDEFLVLEKNTGTVQHVMNGAVGQTVLDLAVNNFSERGLL
                     GIALDPDFASNSYVYLYWSCIATPPAAEDPFFPTQDECAPEPELGEDSEDVLAVPLLG
                     NRVDRFVWDGATLTFDHNLIMLRAFQNDASPEPPGQGDETQPPRGNHDGGVLTFGQDG
                     KLYIFIGDVGRRGQLQNLPCGPIDVYDCPGEDEMVPDDQFGGPEPD"
     gene            1118..1966
                     /locus_tag="VFQ23_13675"
     CDS             1118..1966
                     /locus_tag="VFQ23_13675"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET9907696.1"
                     /translation="MTNIKLPASLVSVHWLAKNLHYENLVILDASIKSVTPIKDDKQD
                     EPAYIPGALRFDIDHEMSDQDTSLPHMMLPADVFTERVETLGINQDSAIVAYDRAGIY
                     SSPRAWCMFRGMGHDQIAVLDGGLPEWIAAGYETVPTLAVPKERGDFTSQPQSQWFVD
                     SNYVLRALTDPTFTVIDARSEGRFRGLEPEPRAGLRSGHMPDAVNIPFTSVLENGKML
                     PKSKLQSIFEKYRDKKMIFSCGSGVTACVVAFAAAQAGHKNLQVYDGSWSEWGQHDSG
                     LPVETG"
BASE COUNT          513 a          486 c          517 g          511 t
ORIGIN      
        1 catccggctc aggtccaccg aattgatcgt ctggaaccat ttcgtcctcg cccgggcagt
       61 cataaacatc tatcggccca cagggaaggt tttgcagttg gccgcgccta cccacatctc
      121 caatgaagat atagagcttg ccatcctgtc cgaaggtaag tacaccacca tcatgattcc
      181 cgcgcggcgg ctgtgtttca tcgccttgac cgggcggttc aggtgacgcg tcattctgga
      241 aagcacgcag catgatcaaa ttgtgatcga aggtgagcgt tgcaccatcc cagacaaaac
      301 gatctacacg atttcccagc agtgggacag ctagtacgtc ttcgctatct tcaccaagtt
      361 ctggttccgg tgcacattca tcctgtgtag ggaaaaatgg atcttcagct gctggaggcg
      421 ttgcaatgca actccagtac agataaacat agctattgga cgcaaaatcc gggtcgaggg
      481 cgatgcccag caggccgcgc tccgagaaat tattgacggc caaatcaagg acggtctgtc
      541 ctacagcgcc attcataacg tgttgtaccg taccggtatt cttctccagt accaggaact
      601 cgtcaggacc gataaaagcc atcgttgtgg gaagattcaa tcctgagacg actgtccgta
      661 cgcccaactg cgggtccaac atttctggcc ctgcttcctg ggcgttggca aaagtggttg
      721 cgccgaaact cataagcaac gaaagcgcaa cgaagagcat tcccactttg cctaaaaaac
      781 ctttttcact catttgaagt tactcctttt aaagcaagat aggtttcacc ttgatggaca
      841 ccaaggtgac ttgactgtag ccaatattga gctctttgag ctggctgaaa tgagattaat
      901 gaagggttag atggggaggc tagcccggta aattattttc taccggcggt atcagtcaaa
      961 agtaagcaaa agccgtgtct gatcaagcaa cacacctcct gcttactaaa ctttacgcat
     1021 tgtagaagat tgtggattta tttagagggt aggactatcc ctacccttct cattttcttt
     1081 ttatcaaggt ctgactataa tttgactggt tttgggtatg acaaacataa aacttcccgc
     1141 ctccctggtt tcggtccatt ggctggcaaa gaatctgcat tatgagaatc ttgtcatttt
     1201 agatgcaagc ataaaatcgg tgacacctat caaggatgat aaacaagatg agcccgcgta
     1261 cattccgggt gcattacgtt ttgatatcga ccatgaaatg agcgatcagg acacatctct
     1321 gccgcatatg atgctgcctg ccgacgtgtt tacagagagg gtagaaacct taggcatcaa
     1381 tcaggatagc gccattgttg cctatgaccg cgcgggaatt tactccagcc cacgcgcctg
     1441 gtgcatgttt cgagggatgg gacacgatca gatcgctgtg ctggatggag gcttgcctga
     1501 gtggatagca gccggatatg agactgtgcc gactcttgcg gtaccaaagg agcgcggtga
     1561 ctttacgtct caaccgcaaa gccagtggtt tgttgattcg aattacgtgt tacgggcact
     1621 gactgaccca accttcaccg tgatcgacgc gcgctctgaa ggacgcttca ggggtctgga
     1681 accggagccg cgagcggggc tgaggagtgg tcatatgccg gacgcggtca atattccatt
     1741 tacgagtgtg ctggaaaatg ggaagatgtt acccaaatca aaacttcaat ccatctttga
     1801 aaaataccgt gataagaaaa tgatttttag ttgtggttca ggtgtgacag cctgtgttgt
     1861 ggcgtttgcg gctgcgcagg cggggcataa aaatcttcag gtttacgatg gctcatggag
     1921 cgaatgggga cagcacgatt caggccttcc tgtagaaaca gggtagtctt tcttattttt
     1981 tcccaatcag caggctcata tcgaaaccga gggagttcat cgtaggt
//
LOCUS       DASTJP010000472         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_7147,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000472 DASTJP010000000
VERSION     DASTJP010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7147"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFQ23_13680"
     CDS             <1..389
                     /locus_tag="VFQ23_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907697.1"
                     /translation="QDQAANIRQYLLPNMQLLGQPLATDEKYQKEDGTYDLQGRLVDL
                     QSTEESQLDPVATFARADLFFSQQRWLVVGSIFLAISLFWLALAEVSGERRRGWQFTL
                     GTAVYFLGILWFVGVELVFLYLRRGG"
     gene            390..1091
                     /locus_tag="VFQ23_13685"
     CDS             390..1091
                     /locus_tag="VFQ23_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907698.1"
                     /translation="MSESSAPPTPAQQNTDQYKTLIVVLTILTTVLAAALAALQSDAS
                     IRADIANRDSQYLAILATGESTRAGLESNYELIVFGDHLKNLQESTILELTALEQEER
                     GDSDAAQASRQLAGIAQSQALEGEKFSIFYTDPRYAPQNENDLPKMDQYGADVFQKAI
                     ELSLQQQDVAERYNIWDRKADSYITALTVLAVAFFLFGLGQALKEARLRLIFAAFGVV
                     VIAFSLLITVTTLLQ"
     gene            1093..1686
                     /locus_tag="VFQ23_13690"
     CDS             1093..1686
                     /locus_tag="VFQ23_13690"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9907699.1"
                     /translation="MLSLLLPILGYLAGSLPFSIWVTRYVKNVDVRDAGSGHATTTNT
                     IRQAGFGWGALVLMLDLAKGFIPTFLAIRYTNEIWVVALTAGLAVVGHCWPVFAQFRG
                     GMGLATLGGALLAANWFSFIVGLGLLLTLTITIRHSARASVFTGIFLSPLFWLIDLKG
                     IEIWIALAGGIVIAYRFLIDWNRQYRELWLDREKPQP"
     gene            1683..>2426
                     /locus_tag="VFQ23_13695"
     CDS             1683..>2426
                     /locus_tag="VFQ23_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET9907700.1"
                     /translation="MKLGIVTDSTSDLPNYLIEQYALEVVPSILILEGKEYADGVGIS
                     REEFYTRLPSFQTQPTTAAPSIGDFSVRYDSLLRRGCDHILSIHATGKLTTIINSARQ
                     AAQDFPTKITIIDSLSMSLGLGFQVLAAAETAELGLQAALDAAESARQRLQVFAAFDT
                     LEYAKRSGRLPSVFATLGGLLNIKPLIELIDGEVKPVGVVRTTKQANEKIMNFLLEMG
                     PLERLAIMHTGAESRAKEFLNAVMQQASQS"
BASE COUNT          569 a          602 c          630 g          625 t
ORIGIN      
        1 tgcaggatca agctgccaat atccgacagt atctcctccc caatatgcag ttgcttggcc
       61 agcccctggc aaccgacgaa aaataccaga aagaagacgg gacgtatgac ttacagggac
      121 ggctggttga cttgcaaagc acggaggaaa gccagctcga cccggttgcg acctttgcaa
      181 gagccgattt gtttttttct caacaacgct ggctggtcgt cggaagcata ttcctggcaa
      241 tcagtctctt ctggctggcg ctggcggaag tcagtggcga gcgccggcga ggttggcaat
      301 tcacgttagg cactgccgtt tattttctgg gcattctatg gtttgttggt gtggagttgg
      361 ttttcctgta tttacggcga ggtggataaa tgagtgaatc aagcgcaccc cctacacctg
      421 cacagcaaaa cacagatcaa tacaagactc tgatcgtggt acttacgatt ctcaccaccg
      481 tactggccgc ggctttggct gcgctgcaat ccgatgcaag tatccgtgcc gatatcgcca
      541 accgcgattc gcaatatctc gctattctcg ctacaggcga aagcacccgc gcgggtttgg
      601 aatccaacta tgaattaatt gtatttggcg atcacttgaa aaatttgcag gaatcgacca
      661 tattggaatt gaccgccctg gaacaagagg agcgaggcga cagtgatgcg gctcaggcaa
      721 gccgccagct ggctggaatc gcacaatcgc aggcacttga aggcgagaaa ttctccatct
      781 tttacaccga cccgcgttat gcaccacaaa atgaaaacga tttgccgaaa atggaccagt
      841 atggggcaga cgtatttcag aaagccattg agctcagtct gcaacaacag gatgtcgcag
      901 agagatataa catctgggat cgtaaagccg attcatatat caccgcatta actgttcttg
      961 cagtggcctt cttccttttt ggtttggggc aggcgttgaa agaggcgcga ttgcgcttga
     1021 tctttgcggc gtttggtgtg gttgtcattg ctttttcact gttgatcacc gtaacgacct
     1081 tgctccaata aaatgttgag cctgctcctg cccattcttg gctatctcgc aggttcgctg
     1141 ccattttcga tttgggtcac gcgctatgta aagaatgttg acgtgcgcga tgcaggttca
     1201 ggtcatgcga caacgacgaa tacgattcgt caggcaggct ttggctgggg cgcgctggta
     1261 ttgatgcttg accttgcaaa aggattcatc ccaacttttc ttgcgatcag atatacaaat
     1321 gaaatttggg ttgtggccct cacggctggt ctggctgttg tcggtcactg ctggcctgtt
     1381 ttcgctcaat tccgcggcgg catggggctg gcgactctgg gtggggcact cctggcagca
     1441 aactggtttt catttattgt tgggctggga ctattgctaa cgcttaccat aacaattcgt
     1501 cacagtgcgc gggcgagtgt ttttacaggc attttcctct ctccgctgtt ttggctcatc
     1561 gatctaaaag gtatcgaaat ttggattgcc ctggcaggtg ggattgtgat tgcgtatcgt
     1621 ttcctcatcg actggaatcg acaatatcgc gaactatggc ttgacagaga gaagcctcaa
     1681 ccatgaaact cggcatcgtc acggattcaa cgtctgatct ccccaactat ctcattgaac
     1741 aatatgcgct tgaagttgtg ccttcgattc tgatactcga agggaaagaa tatgcggatg
     1801 gagttgggat ttcgcgcgaa gaattctata cgcgcttgcc ttcgtttcag actcagccga
     1861 ccactgccgc gccctcgatc ggcgacttct ccgtccgata tgactcgctg ttacgccgcg
     1921 gatgtgacca catcctatcc atccacgcca ccgggaagtt gaccaccatc atcaactcgg
     1981 ctcgccaggc tgcacaggac ttcccgacaa agatcaccat catagacagc ctctcgatgt
     2041 cgctagggct tggttttcaa gtgctcgccg cggctgaaac tgcagaattg ggtttgcaag
     2101 ccgcgttgga tgcagccgaa tcagcgcgtc agcgcttaca agtctttgct gcattcgaca
     2161 cattggaata cgcgaagcga agtgggagac tcccttctgt ttttgccacc ctgggaggat
     2221 tgcttaatat caaacctttg atcgaattga tcgatgggga agtcaaacct gtcggtgttg
     2281 ttcgtacaac gaaacaagcc aacgaaaaaa ttatgaactt tttattggaa atgggtccac
     2341 tggaaagatt ggcgatcatg catacgggcg ctgaatcacg tgcaaaggaa ttcctaaatg
     2401 cggtgatgca acaagcaagc cagtct
//
LOCUS       DASTJP010000473         2937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1139599, whole genome shotgun sequence.
ACCESSION   DASTJP010000473 DASTJP010000000
VERSION     DASTJP010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139599"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFQ23_13700"
     CDS             complement(<1..281)
                     /locus_tag="VFQ23_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907701.1"
                     /translation="MTPKPSGTVAFLFTDIEGSTVLAQRHPDKLVVGDSFSAAFHSAS
                     DALGAALDAQIQLHYEDWGDAPIKVRMGIDTGIAELADDPHTQGAYSGYA"
     gene            836..1216
                     /locus_tag="VFQ23_13705"
     CDS             836..1216
                     /locus_tag="VFQ23_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907702.1"
                     /translation="MNLNETHSGNPSASNWQVLGQLELMADSQAHGYLRDQLVEILKP
                     LHLHENFLNKLLLSAEEYTTRALSSITALGHGHLHLVIFTQPECVSASESWGFFRIEK
                     IDNAEQNPVHPDHVVEFYLYLEGL"
     gene            complement(1192..>2937)
                     /locus_tag="VFQ23_13710"
     CDS             complement(1192..>2937)
                     /locus_tag="VFQ23_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9907703.1"
                     /translation="DAAALREICQLVDGMPLAIELAASWTRMLTCAEIAHEIQSDVNF
                     LATSSGDTSERHRSVRATFEHSWKLLSLGEQTVLQRLSIFKGGFTREAAEYVTGANLT
                     LLSALVSKSLLHRTEQEHYDLHELVRQYSLEYLKMNESEYMETQDRHSEYYSGLLKKR
                     GERFKSADQPTVARELAAEIANLRQAWRWAGDRVQAMQVGQAADTLFWLYESRCDCRE
                     GVTLFGYVAHRLETAASADETQLITLARVMSYQAFFCLRQGMHPQSKDLLERSIAILR
                     PMAENGSLAARDALSDTLAFLGMLTVSLGEYTSGNRFLNEGLEIKRANKDGWGIAFCL
                     RQLGVLGFYQGAYDEADRLLNEGLQVSRSLGNAWAIAYSLDFLSTAAYARGAYTEAER
                     LLREGLVLSQQVGDRFTTAYALNGLGMVKHSLGEHAEAQRLLEDSISIWREIGDLASM
                     AQSLINLGNVFLATNTHLEAQNCFREALSVAKDAGLVPVMLDAMIGEAEIRAASDDIK
                     PAFEAAWMVSQHPSSSHATRTRAGVMCAKLEPQLSHDEVGHIKANKGTLESMIHEIPA
                     VLTEYPLGYSPSRYK"
BASE COUNT          718 a          776 c          712 g          731 t
ORIGIN      
        1 gcgtatcctg aataagctcc ctgagtatgg ggatcatctg ccaattcggc aatccccgta
       61 tcaatcccca ttcgcacttt gattggagca tccccccaat cctcatagtg aagctgtatt
      121 tgtgcatcga gtgccgcgcc caatgcatcg ctggcggagt ggaacgctgc tgaaaatgaa
      181 tcccctacca ccaacttgtc aggatggcgt tgagcgagga cagtgctgcc ttctatgtcc
      241 gtgaaaagaa aggcgacggt tccactcggc ttaggggtca taatccatgc tgttgagaaa
      301 tcgtatcatg acataaataa ttccttttgg aaacggaatt gctactcgtt tcatggctgg
      361 accttcaatc ccgtggagac gaacagtaga gcagatatcg acaaccacta atatcggtgg
      421 aatgtgtagc ggactaaaag ataaatatga acgtttgaga tcaaaaattg gagagtatct
      481 gaccagtcgc aagtgtggcc tccagcggac ttcggtgccg gtctgttttt tcgtttatta
      541 ggatggattt gtactagttt ttgtccgatt ttctgtccaa agaaaataca ccccgcagga
      601 ctttagtgtt tgtataaagg ggacagttca atgcaataaa ggagaaaaca ccatgtccac
      661 tgttgccggc accatgtcca aggaaaaagg attcataaca tttgtccgaa atcacccgct
      721 cgtttcattt ttctttctcg tctacggggc atgaggatcg tgatctgttg ccgggtcatg
      781 aatagacttg gacttcagca tgttccaaag ccaaatattg actctagtga actagatgaa
      841 cttgaatgaa actcattccg ggaacccgtc cgcctccaac tggcaggtac tcgggcaact
      901 tgagctgatg gcagactcgc aggcacatgg atatctccgt gaccagctcg tcgagatcct
      961 caagcccctc catttgcatg aaaactttct gaacaaactg ctgctatcgg cagaggaata
     1021 tactacacgc gccctgagct caattacagc tttagggcat ggtcatctgc atctggtcat
     1081 ttttactcag ccggaatgtg tctccgccag tgaatcatgg ggattcttcc gcatcgagaa
     1141 gatcgacaat gccgaacaaa acccggttca tcccgaccat gtagtggagt tctatttata
     1201 tctggaagga ctatagccca aaggatactc tgttagtacg gcaggaattt catggatcat
     1261 ggattcgagt gtgcctttgt tagccttgat gtggccaact tcatcgtggg aaagttgtgg
     1321 ttccagcttg gcgcacatta caccggcacg cgtgcgcgtt gcatgagaac tggagggatg
     1381 ttggctgacc atccaggctg cttcgaacgc gggttttata tcatcactcg ctgcgcgaat
     1441 ttctgcttca ccgatcatag catccagcat tactggaacc agcccggcgt ctttcgcaac
     1501 ggataaagct tcgcggaaac agttttgggc ttcaaggtga gtgtttgttg ccaaaaatac
     1561 attgcccaag ttgatcaacg attgcgccat acttgccagg tcgccgatct cgcgccaaat
     1621 ggaaatgcta tcttcaagca gccgctgcgc ctcggcgtgc tcgcccaaac tatgtttcac
     1681 cataccaagt ccgttgagcg cgtacgctgt cgtgaagcga tcgccaacct gctgactcaa
     1741 caccagtccc tcgcggagaa gcctttccgc ttctgtgtac gcgccgcgcg catatgccgc
     1801 tgtgcttaga aaatccagcg aataagcaat cgcccacgca ttacccagtg agcggctgac
     1861 ctgcaaccct tcgtttagca aacggtctgc ttcatcatac gcaccctgat aaaagcccag
     1921 cacgcccaat tgtcgcaggc agaacgcgat tccccaacca tccttgtttg cgcgtttgat
     1981 ttccaggcct tcattcagga aacgattgcc gctcgtgtac tcacccagcg agacggttag
     2041 cattcccagg aatgctaacg tgtcggagag tgcatcacgc gctgcaagtg aaccgttctc
     2101 tgccatgggg cgcaatattg caatactgcg ttccaacaaa tctttggatt gtggatgcat
     2161 gccctgccgc agacagaaga aagcctgata tgacatcaca cgtgccaacg tgataagctg
     2221 cgtctcgtca gcgctggcag cagtctctag acgatgcgcc acatatccga agagcgtaac
     2281 accctcgcga caatcacaac gcgactcgta caaccagaag agggtatcgg ccgcttgacc
     2341 aacttgcatt gcctgtacac ggtcccccgc ccagcgccat gcctgccgca aattggcaat
     2401 ttcggcggca agctcgcggg ccacagttgg ttggtcagcg ctcttgaacc tttcacctcg
     2461 ttttttcaac aaaccggaat aatattcact gtgacgatcc tgcgtttcca tgtactctga
     2521 ctcgttcatt ttcaaatact cgagcgagta ttggcgcacc aattcatgca aatcgtaatg
     2581 ttcttgctcg gtacggtgca gaagggattt ggataccagg gcagatagca atgttaggtt
     2641 tgcgcctgtc acatactccg ctgcctcgcg tgtgaaacct cccttgaaaa ttgacaatcg
     2701 ctgtaaaacg gtttgttctc ctaaggaaag gagcttccat gaatgttcga atgtggcccg
     2761 aacggaacga tgcctttccg atgtgtcgcc tgaggaagtc gcaaggaagt tgacatcgga
     2821 ttggatctcg tgtgcaatct ccgcacaggt gagcattctg gtccatgagg cagccaattc
     2881 gatcgctagc ggcatcccgt ccacaagctg acaaatttct cgcaaggccg ctgcatc
//
LOCUS       DASTJP010000474         7673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1126819, whole genome shotgun sequence.
ACCESSION   DASTJP010000474 DASTJP010000000
VERSION     DASTJP010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126819"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFQ23_13715"
     CDS             complement(<1..404)
                     /locus_tag="VFQ23_13715"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET9907704.1"
                     /translation="MTVSPAMRRYAAEIYRLQQDHEQVSLSLLSSHVEASAQAISTMV
                     KRLNKNGFLVHEPYRGVRLTPDGERMAMPSLRRHRLTEVFLVKVMKYDWASAHELSDV
                     FERGVNEELEDRMDELAGYPTRCPHGEPIPSK"
     gene            601..1647
                     /locus_tag="VFQ23_13720"
     CDS             601..1647
                     /locus_tag="VFQ23_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9907705.1"
                     /translation="MGKIKISIIFILITILASACGAAPTATAAPIAESGVISGDLVIY
                     TGRTEPLLQPVIDAFKTKYPNVNILLKAGSNSELANALLEEQANPQADVFITTELFTV
                     QSLAGQGIFQAYKPVGADQLPAEFLGADNLWTGLTRRARVIIYNADLVSQDELPTSIF
                     DLTDPKWKGQIAAAGSTNGSMQTQVASLRQLIGEEETETWLKGLLTNEVTFFGGHTDV
                     RKAVGAGEFKLGLVNHYYYHLQKAEGSNVGIIFPDQGEGQIGLITNATAAGIVAGAPH
                     LPAAQAFLDFLVSPEGQQLFAEQNYEYPLLSGVALHSDVLSLDGLRLAEVDVVKAAED
                     FEATFDLMEKVALP"
     gene            1697..3337
                     /locus_tag="VFQ23_13725"
     CDS             1697..3337
                     /locus_tag="VFQ23_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET9907706.1"
                     /translation="MEATDRHRFNLLRLRSGTISPRLAIAAGLVALFVAIPLAYIFIR
                     ALGAEPEAWGRLLQNRIWKLLGNTLVLVIAVTSGALVTGVSMAWFTERTDLPGRKIFR
                     WMLALPLAIPAYIGGIVHLALLRPRGGYIPQALENIFGQPVFTPSPLGFWGAAFILTL
                     FTFPYVYLLSGAAFRSLHASLEEAARTFGRTPFQTLLQVTLPALRPGLAAGALLVALD
                     ILAEYGTVALLRYETFSSAIFVQLSGRYDRSAASVLSGILVALAILILWSELRWQGQA
                     RFTQMESNWRPAPPAILGRWRVPAFLLVLGVVSASLLIPVIVLIVWSVQAFRDPGTLV
                     TVFRTGSQGFGSYLWNSLWSSGLAAFIAVILSMPVALLSVRYPNRFSRFISRFCQVGY
                     AIPGVVIALSLVLLVNRLLPFLYATPFVVVIAYVLRHMPQAVRASEAALNQLSPSLEE
                     ASRTLGRTSIQTFMQVTVPLILSGLLAGGSLVFLTSLKELPATLLLRPAGFDTLAVRV
                     WMWATEGYYLQAAPVALLLVFASSLPLYFLLRREKIFR"
     gene            3334..4374
                     /locus_tag="VFQ23_13730"
     CDS             3334..4374
                     /locus_tag="VFQ23_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907707.1"
                     /translation="MTALEVRNLVKHFQGTPQSAVNNISFELDGGEILALVGPSGCGK
                     TTTLRIIAGLERPDSGTVCLNDRFVVSESVFIPPEKRGVGMVFQDHALFPHLTVSDNI
                     AFGLRGQTPAEIRQTVGEMLHLVGLLPLSKRYPHALSGGERQRVALARALAPRPVLVL
                     MDEPFSSLDADLRVEMREHVRRILKSRQATVIFVTHDQDEALYMGDRLAVLQNGQIEQ
                     IGTPEEIFHESNTRFVSEFMGDSDFLIGKVTRRGILTEIGLLNQAVDLPVSTPVEIAL
                     RADDVDFMVDGSGNSVIVERFFRGAFNLYRLRLDSGQTLHASTSHTQILPVGKRVRAF
                     ISAEHPLTIFSE"
     gene            4567..5646
                     /locus_tag="VFQ23_13735"
     CDS             4567..5646
                     /locus_tag="VFQ23_13735"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HET9907708.1"
                     /translation="MVFKSKFYRAAIILLLLLSACAPAVTPDTHTDHTQNDLAGIKTY
                     LLEKSSELTTSSKLLKDASDTYYELAQSAGFDYATLWESSPAEVSTALTDAKSAWMAA
                     SPLYEQMEGIVAGTPSLAEYDVILDAGTSAEEDPEGAVPFDLTLPDGRVLLKPGNLFG
                     VLESTLWGTYADFTTGIEADLDRNGSVDFGEKLPDAKVLKAAADALDQYTTELGAASS
                     EWEPTESDAFTALVVMVPTMSEYFNSWKNSRFIMGDASEQRDFVVISRLADIQNILGS
                     LQVVFGEVKPLVESTDSAQAAQIEQSLADLKSFVGDVYNKEQGGYQHSPEEADVLGAE
                     AQNRATAIAGQVAQIAAKLNISIAE"
     gene            5646..>7673
                     /locus_tag="VFQ23_13740"
     CDS             5646..>7673
                     /locus_tag="VFQ23_13740"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HET9907709.1"
                     /translation="MHKFLNHFLISTLLATLAVSSFLGVAHAATLSPAQVAETIRASL
                     VQAQLSLPGDPNHSADLLQDAEAAYQTRLSAHISDSHPDAHLRILSAFAALTDSISNS
                     DATSFAAARAQVWTGILAGSYAIVEEAIQSGDGLTAQEWLPLREFRTATRFSRPNVAA
                     TVAVESLISGETSADDALLSIQADVLDTYQARMTESLRDLQTADTNGFASRRAELATL
                     AEGYFFILSPAYLEQRGTASLSTVEIAFSELRTSAIENPELLKDKLTEVETALDNFRA
                     APLSPAEQSRRAGQLLRFLSLVPVEYGRGVVDGRVTRDIEIQEAITFHNGAYAAFSDL
                     RDLLDMLDHTTTDQANLLFVSLGKQLSETGTGKSVASPESIRSQTDELSTLVETLIPA
                     EWLSNSAQGDFDVIASMLDQMLSAVRNGEYDLAESSRLDAYAIMETGPEARLIVFAPE
                     LKLQLEELFWNGQGEHKGLAYLIKNRAAYSEIKTTHTQLDVSLTEAQAVLGRNTAPAA
                     IAMNAGIIVFREGLEAVVILASLMSSLKSIEERKYRKPMWLGVWLALLASALTWLLAH
                     GILQSLARYGEKLEAIVSVIAIAVLLLIMNWFFHNTYWTSWLANFHSQKRRLLSQETG
                     LWLGLITLGFTSVYREGFEIVLFLQALVLEGGVGVVVIGVLLSLAAVNLVGM"
BASE COUNT         1751 a         1918 c         2018 g         1986 t
ORIGIN      
        1 tctttactgg ggatgggttc tccatgaggg cagcgagttg gatatcccgc caattcatcc
       61 atgcgatctt cgagttcttc attgacgccg cgctcaaata cgtctgatag ctcatgagct
      121 gacgcccaat cgtatttcat caccttgacc aaaaagactt ctgtcaggcg gtggcggcgc
      181 agagagggca tggccattct ttcgccgtca ggggtaagac gaacgccgcg gtagggttcg
      241 tgcactaaaa atccattctt attcaaacgc ttgaccatcg tggaaattgc ttgcgcggag
      301 gcctccacat gcgaacttaa tagcgaaagc gatacctgtt catgatcttg ttgcaggcgg
      361 taaatctcag cggcgtaacg ccgcatggcg gggctcacag tcataccgtt aatcaaactc
      421 cagaaaacat caaccaggtt gaaaactggt tgcaatggga tatagggtaa atttaataca
      481 aatatctaga ttattccgat tgtgaaaatt atactacgcg ccgtaagtag ccgatgaata
      541 ctgctcggtt tattgcagat atttgttcaa tattcaaaat tctcaaggag aacttctctc
      601 gtgggtaaga tcaaaatttc cattattttc atcctgataa ccatcctggc gagtgcctgt
      661 ggagcggctc caactgcaac ggcggctcct atcgcggaat caggcgttat ttccggcgat
      721 ctcgtcattt acacagggcg taccgaacca ctgcttcagc cagtgatcga cgcgttcaag
      781 acgaaatatc ccaatgtcaa tattctgctc aaggcgggga gtaacagtga actggccaac
      841 gcgctgctcg aagaacaggc aaatccacag gccgacgtgt tcattacgac ggagttgttc
      901 acggtgcaat ctcttgccgg gcagggcatt tttcaggcat acaaaccagt tggtgcagat
      961 cagcttccgg ctgagttcct gggcgcagac aacctgtgga ccgggcttac acgtcgggcg
     1021 cgggtgatca tctataatgc cgatctcgtt tcacaggatg aacttccgac ctccattttt
     1081 gatttgaccg atcccaaatg gaagggccaa attgccgcgg cgggttcgac aaatggttcc
     1141 atgcaaacgc aggttgcctc tctcaggcaa ttaattggcg aagaagagac agaaacctgg
     1201 ttgaaaggct tactgaccaa tgaggtgacc ttttttggcg gtcacacgga cgtccgcaag
     1261 gccgtgggtg ccggcgaatt caaactaggg ctggtcaacc actattatta tcatctgcaa
     1321 aaggcagagg gaagtaatgt gggaatcatt ttccctgacc agggcgaggg gcaaattgga
     1381 ctgatcacca atgcaactgc cgctggaatt gtcgctggtg cgccgcatct cccggcagcg
     1441 caggcctttc tggattttct ggtctcaccg gaaggacaac aattgtttgc cgaacaaaat
     1501 tatgagtatc cacttttgag tggtgtggca ctgcattcag atgttctttc actggatggg
     1561 ctccgtcttg cggaggtaga tgtcgtcaaa gctgcagagg atttcgaagc cacattcgat
     1621 ctaatggaga aagttgccct cccatagacc gatggtttcc taaatatcca ctaaaattgt
     1681 ccttcggata actgacgtgg aagcaaccga tagacatcgc ttcaaccttc tgcggttaag
     1741 atcaggtacc atcagcccca ggcttgccat cgccgcgggg ctggtggctc tattcgttgc
     1801 tatcccactt gcttatattt tcatccgcgc gcttggcgca gaacccgagg catgggggcg
     1861 attattacaa aatcgcatct ggaaattact gggtaacaca ctggtgcttg tcattgccgt
     1921 gacgagtgga gccctagtaa caggagtgag catggcgtgg ttcaccgagc gcaccgacct
     1981 gcccggcaga aaaatatttc ggtggatgct cgccctgccg cttgccatcc cagcctatat
     2041 cggtgggatc gttcacctgg cactgctgcg cccacgtggt ggatacattc cccaggctct
     2101 tgaaaacatt tttggtcaac ccgtgttcac gccgagcccg cttggttttt ggggtgcagc
     2161 atttattctc actctattta catttcccta tgtttacttg ctcagcggcg cggcgttccg
     2221 ctccttgcat gcttctctgg aagaagcagc gcgcaccttt ggtcgaactc cgtttcagac
     2281 gctcctccag gtgaccctgc ctgcgttacg ccccgggctc gcagcgggcg cattgctggt
     2341 ggcgttggat attttggcag agtatgggac agtcgctttg ctgcgctatg aaaccttttc
     2401 ctccgcaatc tttgttcaac tctcaggacg gtacgacagg tcggcagcgt ctgttttaag
     2461 tggcattctg gttgccctgg cgatattaat tttgtggagc gaattacgtt ggcagggaca
     2521 agcacgcttt acgcaaatgg aaagtaactg gcgccccgcg cctccagcca tactcggtag
     2581 atggcgcgtg cccgcgtttt tgttggttct tggtgtcgtc tctgccagtc tgcttatccc
     2641 agtgattgtg ctgatcgtat ggagcgtcca ggcttttcgc gatccgggaa ccctggtaac
     2701 ggtatttcgg actggctcgc agggtttcgg cagttatctg tggaacagcc tgtggagttc
     2761 agggctggca gcttttatcg ctgtgatctt atccatgcct gtggctttac tgtctgtgcg
     2821 gtatccaaat cggttctcca gattcatttc ccgtttttgt caggtgggat acgcaatccc
     2881 aggtgtagtc attgctctca gcctggtatt gctggtcaat cgtctgcttc cgtttctgta
     2941 tgccacgccc tttgtcgttg tcattgccta tgttctaaga catatgccac aggcggtacg
     3001 cgccagcgaa gcagccttga atcaactttc accgtcgctc gaagaagctt cacgcacgct
     3061 gggacgcaca tcgattcaaa cgttcatgca agtgaccgtg cctctcattc tttccggatt
     3121 attagccggt ggatcgctgg tctttcttac atcgctcaag gaattgcctg ctaccttatt
     3181 gttgcggcct gctggctttg ataccttggc ggtccgtgtt tggatgtggg caacagaagg
     3241 ctactattta caagccgcac cagttgcgtt gttacttgtt tttgcgtcgt cactcccgtt
     3301 atacttcctc ctgcgaagag agaaaatatt tagatgacag cattggaagt acgcaatctc
     3361 gtcaagcatt ttcagggcac cccacaatcg gccgtgaaca atatctcctt tgagttggac
     3421 ggcggcgaaa tcctggcgct agtgggtccc agtggatgtg gaaaaaccac taccctgcga
     3481 attatcgctg ggcttgagcg cccagattct ggaacggtat gcctgaatga ccgatttgtg
     3541 gtatctgagt cggtttttat tcctccggaa aaacgtggag tcggtatggt ctttcaggat
     3601 catgcattat ttccacatct aaccgtttca gacaatatcg cctttggctt acgagggcaa
     3661 actcccgcgg agatccggca aacggttgga gaaatgctgc acctcgtcgg gttgctgccg
     3721 ttgtcgaaac ggtatccgca tgccttgtct ggcggtgaac gacagcgagt tgccctggcg
     3781 cgggcgttgg cgccacgccc ggtgctggtg ctcatggatg aaccgttcag cagtctggac
     3841 gccgaccttc gcgtggaaat gcgcgagcat gtgcgccgca ttctcaaatc caggcaggcg
     3901 acggtgatct ttgtcacgca tgatcaggat gaagcccttt acatggggga tcgtctggcc
     3961 gtgctgcaaa acggacagat tgaacagatc gggactccgg aagaaatttt tcatgaatcg
     4021 aatacgcgct ttgtttccga gtttatggga gatagcgact ttctcatcgg caaagttaca
     4081 cgtagaggaa ttctgactga aattggtctg ttgaaccagg cggttgactt gcctgtttcc
     4141 acgcccgttg agatcgcact tcgcgccgac gatgttgact ttatggtgga tggcagtggg
     4201 aacagcgtaa tcgttgaacg gttttttcgc ggggctttca atctttatcg cctgcgcctg
     4261 gactccggtc agaccctgca tgcttccaca agccatacac agatcctgcc ggtgggcaag
     4321 cgcgttcgcg cctttatcag cgcggaacat ccgttaacaa tcttttcgga gtgacaacgc
     4381 tgaagttagt catatttcaa aatcatcaac caggttgaaa atggattcca agggggtata
     4441 gagcgaattt aataccaaca cctagattat ttttcttgtg aaaactatac tgcacaacgc
     4501 ttgagcgaaa ctcactataa caggctgagt ttcgctcaaa gttgttcatt taagaggaga
     4561 accataatgg tttttaagtc aaagttttat cgcgcagcaa ttattcttct actacttttg
     4621 agcgcgtgtg cacctgcagt tactccggat acacataccg atcataccca aaacgacctg
     4681 gctgggatca aaacctattt actggagaaa tcctcagagt tgacaaccag ttcgaagtta
     4741 ctgaaagacg cgagcgacac atattatgag ctggcgcagt ccgctggttt tgattacgca
     4801 actttatggg aatccagccc ggcagaggtg agcacggctc tcacagatgc gaagtccgcc
     4861 tggatggcgg ccagcccact gtatgagcaa atggaaggca ttgtggctgg cacacccagc
     4921 ctcgctgagt atgatgtcat tctcgatgcg gggacctctg ctgaagaaga ccccgagggt
     4981 gcagttccct ttgacctgac tctgcccgat gggcgtgttc tgctcaaacc aggtaacctc
     5041 ttcggtgtgt tggaaagtac gctgtgggga acttatgctg atttcacaac tggtatcgaa
     5101 gctgacctgg ataggaatgg ctcggttgac ttcggtgaaa aactaccgga tgcgaaggtg
     5161 ctcaaagctg ccgcggatgc cctggatcaa tatacaactg aacttggagc cgcttcttcc
     5221 gagtgggagc cgaccgagtc cgatgctttc acggcgctgg tggtaatggt acccaccatg
     5281 agtgaatact tcaactcatg gaagaactcc cgatttatca tgggtgatgc ctcggagcag
     5341 cgcgactttg tagtgatttc acgtctcgct gatattcaaa atattcttgg tagtttgcag
     5401 gtcgttttcg gtgaagtgaa gccgttggtt gaatcaacag actcagcgca agccgcgcaa
     5461 atcgaacagt cgttagcaga cttgaaaagt tttgtcgggg atgtatataa caaagaacag
     5521 ggcggctatc agcactcgcc tgaggaagcc gacgtgctgg gtgcggaggc acagaaccgg
     5581 gcaactgcca ttgccgggca agttgctcaa atcgccgcaa aactaaatat ttccatcgca
     5641 gaataatgca taaatttctt aatcattttc tgatttctac gctgcttgca acgctggcgg
     5701 tttccagttt cctgggagtc gcacatgcgg cgactctctc accggcgcaa gttgcagaaa
     5761 cgattcgagc ttctttggtg caggcacagc tttccttgcc aggtgacccg aatcattctg
     5821 ccgatttgtt gcaggatgca gaagcggctt atcaaaccag actttcagca cacatctctg
     5881 attcacatcc ggatgcccat ctgagaatcc tttctgcttt tgccgcacta acggatagta
     5941 tttcaaatag cgatgcgacc tcctttgctg cagcccgcgc acaagtctgg acaggaatcc
     6001 tcgctgggag ctatgcaatt gtggaagaag caattcaaag tggcgatgga ctgactgcac
     6061 aggaatggct gcctttgcgc gaatttcgca ctgcgacgcg tttctcacgc ccgaatgtag
     6121 cagcaacggt tgctgtggaa agtctgattt cgggggaaac atctgccgac gatgctttat
     6181 tatctatcca ggccgatgtg ctggatacgt atcaggcacg catgaccgaa tccctgcgtg
     6241 accttcaaac agcagacacc aacggatttg cttcgcgtcg cgccgaacta gccaccttag
     6301 ccgaaggata tttctttatc ctttcgcccg cttatcttga acaacgaggc accgcttctc
     6361 tttcaaccgt ggaaattgca ttctctgaat taagaaccag tgcaattgag aatcctgaat
     6421 tgctgaagga caaattgaca gaagtagaga ctgcactgga caatttccgc gccgcgccgc
     6481 tcagcccagc cgagcagtct cgccgtgccg gacagttact gcgatttttg tcactggtgc
     6541 ctgtcgaata tggacgcggc gtcgtggacg gccgcgtcac gcgtgatatt gagatccagg
     6601 aagccattac attccataac ggggcctatg cagccttctc agatttaaga gatcttcttg
     6661 atatgttgga tcacactact accgatcaag ccaatttgct ttttgtgtca cttggaaaac
     6721 aactttcgga aacaggaaca ggcaaatcgg ttgccagccc tgagtccatc cgatctcaaa
     6781 cggatgagct ttctactctt gtggaaacac taatccctgc cgagtggctc agcaacagtg
     6841 cacaaggtga ctttgatgtg atcgcatcca tgctggacca gatgctgagc gcggtcagaa
     6901 acggcgaata tgaccttgct gagtcatccc ggctggatgc ctacgccatc atggaaaccg
     6961 ggcctgaagc caggctgatt gtgttcgcac cagaactgaa attgcaattg gaagaattgt
     7021 tctggaacgg gcagggtgaa cataaggggt tagcctatct catcaagaat cgtgctgcct
     7081 attcagagat caaaacgacg catacacaat tggatgtatc gttgaccgaa gcacaggcgg
     7141 tgctaggcag gaacactgca cccgccgcga tcgcgatgaa tgcgggcatc atcgtcttcc
     7201 gggaagggct ggaagctgtg gtaatccttg cttcgttaat gagcagtcta aagtccatag
     7261 aggagcgcaa atatcgcaaa ccgatgtggc tcggggtctg gttggcgttg ttagcttctg
     7321 cattaacgtg gctgttggcg catggcatcc tgcaatcact ggcacgctat ggagaaaagc
     7381 tggaagccat tgtctcggtt attgccattg ctgttttgct cctgatcatg aattggtttt
     7441 tccacaatac ctattggaca agttggctgg caaatttcca ttcacagaaa cgcagactgt
     7501 tgagtcagga gaccgggctt tggctggggt tgatcactct tggttttacc agcgtctacc
     7561 gcgaaggatt cgagatcgtt ttgttcctcc aggcgctcgt cctggaaggt ggcgtggggg
     7621 tggtggtcat cggtgtgctt ctgtctctgg cggctgtaaa tcttgtggga atg
//
LOCUS       DASTJP010000475         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1415319, whole genome shotgun sequence.
ACCESSION   DASTJP010000475 DASTJP010000000
VERSION     DASTJP010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415319"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFQ23_13745"
     CDS             <1..418
                     /locus_tag="VFQ23_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9907710.1"
                     /translation="LEMGADDYVTKPFSLRELVSRIRAQLRRAYGDYASTESSVLLVA
                     DLALDQITGQLRRGDQAINLTPIEFRLLSHLARHRGQALTRGQIVEAVWGYAPDLESE
                     KTVNVHIRRLREKIELKPEEPAIILTVPGIGYRLVG"
     gene            734..1915
                     /locus_tag="VFQ23_13750"
     CDS             734..1915
                     /locus_tag="VFQ23_13750"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9907711.1"
                     /translation="MSKRALFIFILVGILAISGAGYFGLATTQEPVPTPTPQTVSVEM
                     CDVDQTVTAPGNLVNVNEAEVSMPATGKLSAVNVRVGDRVQEGQALAELDPLTTSQAQ
                     LSLLEAQEELEKLQRQRTALDYPRATNDFIKDYRKRVKAAKDTVDSLNDSYRNTEDPM
                     MRSQILAALTNARTELKTLESNLNWYVSNPTESEIVQADSELALAQAKYDVAKALLEN
                     LEIKAPFDGIVFEVSAESGQTYQAESVLFKIGDPKALEVIANITEEDYPIVSVGQVVE
                     VFFDARADVTVTGKVDRIIPKRIEGDRPRYNIFITLDEVPDGLADGMTADTAITIQKR
                     EAVLCLPRSVVRASGVDEVSLKVWANNAIETRLVTVGLRGDSNVEILSGLQEGDQVVI
                     Q"
     gene            1912..2625
                     /locus_tag="VFQ23_13755"
     CDS             1912..2625
                     /locus_tag="VFQ23_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907712.1"
                     /translation="MNDSSNSVIRTAGLTREYVMGQAILKALRGVDLTVRQGEFLALM
                     GASGSGKSTLLSLIGLLDRPTSGQYWLENVEVSSLSRDQLATIRGRRIGFIFQNFNLL
                     PRFSAWENVALPLAYRKGEFRNDQQYKKAMEVLTRVGLDRRAGHQPMELSGGERQRVA
                     IARALVTQPAVILADEPTGNLDSKTGKEIMSLLGELHAEGRTIIIVTHDMNIARHANR
                     ICIMQDGILMEEESLHVAG"
     gene            2612..>3079
                     /locus_tag="VFQ23_13760"
     CDS             2612..>3079
                     /locus_tag="VFQ23_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9907713.1"
                     /translation="MSLADLIRTSLRGVSANKLRAALTMLGIIIGVASVIAMLALGNG
                     ARAAVDARFRFLGANQVQISAMMAIDDEQFVSAGKNLSYEDGLLMPGEVELVGSVDMS
                     IGAAAKARFGRATLDINISGITANALERIVSAGEVQPVNRQDGEALTIDSYIAG"
BASE COUNT          733 a          774 c          859 g          713 t
ORIGIN      
        1 gctggaaatg ggcgcggatg actacgtcac caagccattc agtttgcgtg aacttgtctc
       61 acgaatacgc gctcaattaa ggcgcgccta cggcgattac gcttcgaccg aatcttctgt
      121 tttattggta gcagacttag cgctggatca aatcactggg cagttgcggc gcggcgatca
      181 agccataaat ttaactccca ttgagtttcg tttattgtcg catcttgcga gacaccgcgg
      241 gcaggcattg actcgcggtc agatcgttga agccgtgtgg ggatatgcac cagatctaga
      301 aagcgagaaa accgtcaacg tgcacatccg acgtttgcgt gaaaagatcg aactcaagcc
      361 cgaagaacca gcgattattc tcactgtccc tggaatcggc tatcgtttag taggatagga
      421 tcgtcctccc ctaaattccc tctttggaat ttgagggagg gtcccgccga aggcagggac
      481 ggaggggggt ggtgattaca aaatcttaat cacctcacaa ttcagttgaa acctgggcaa
      541 tatagactta cagacaaggc aagtccttgt ctgtaatttt tttccgttgg tcgagtagcg
      601 cgtagtgcat atcgagacca acatgttatc ggaaaatttt tgatttgctt tccaagctgt
      661 ggtctcgata tgacactctt tagcattcgt gtctactcga ccaccgaggc caacttttct
      721 cgggagtttc atcgtgagta aacgcgctct gttcattttt attttggttg gcatcctggc
      781 aatttccggg gccggatact tcggcctggc aacaacacag gaacctgtgc caactcctac
      841 ccctcagact gtttccgttg aaatgtgcga tgtggatcaa acagtcaccg cgccgggcaa
      901 tctggtcaat gtgaatgagg cagaagtgag catgccagcc acggggaaat tatcggcggt
      961 caatgtacgc gtcggggata gggtgcagga aggtcaggcc ctcgcagaac ttgatccgct
     1021 gacgacttca caggcacagt tgagtctgct cgaagctcag gaagaattgg aaaaacttca
     1081 aagacagcga accgcgcttg actatccacg tgccaccaac gatttcatca aggattaccg
     1141 caagcgagtc aaagccgcca aggacacagt cgactcattg aatgactcct acagaaacac
     1201 agaggatccc atgatgcggt cacagatcct ggcggcgctc accaacgcca ggaccgaact
     1261 gaaaaccctt gaatcaaatt tgaactggta tgtcagtaat cccaccgaaa gcgagattgt
     1321 tcaggctgat tcggagctgg cgctcgccca ggcaaaatat gacgttgcca aagccctgct
     1381 ggagaacctc gagatcaagg ctccatttga tggcattgtc tttgaagtct ctgccgaatc
     1441 agggcagacc tatcaggccg aatccgttct attcaaaatc ggcgacccaa aggcactgga
     1501 agtgatagcc aatattacag aagaggatta tccgattgtc tcagtaggcc aggtggtgga
     1561 agtgttcttc gacgcgcgcg ctgatgtcac cgtcactggc aaagtggatc gcattatccc
     1621 taaaagaatc gaaggcgacc gcccacgcta taacatcttc atcaccttag acgaagtgcc
     1681 agacgggctg gcggacggta tgactgccga taccgctatc acgattcaaa agcgggaagc
     1741 ggtgttgtgc ctgccgcgtt ctgtggtccg tgcatcgggt gtggatgaag tttcgctgaa
     1801 ggtttgggcg aacaatgcga tcgagactcg gctcgtcact gtcggcttgc gcggcgactc
     1861 aaacgtggaa atcctctcgg gattgcagga aggcgaccag gtagtgattc aatgaacgat
     1921 tcgtcgaact ccgtcattcg gaccgcgggc ttaacccgcg agtacgtgat gggtcaggca
     1981 attctgaagg cattgcgagg cgtagacctg acggtgcgcc agggtgagtt cctggcattg
     2041 atgggtgctt ccgggagcgg caaatcaacc ctgttgagtc tgattggctt gttagatcgc
     2101 cccacctccg gtcaatattg gcttgagaat gtggaagtct cctctcttag tcgcgatcaa
     2161 ttagcgacga tacgcggccg gcgcatcgga tttatttttc aaaacttcaa tttgcttcca
     2221 cgtttttcag cctgggaaaa tgtcgcgcta ccgctcgctt atcgaaaagg ggaatttagg
     2281 aatgaccagc aatacaaaaa agcgatggaa gtgcttaccc gcgtcgggct ggaccgtcgc
     2341 gccggtcacc aacccatgga gctttccggt ggcgaacggc agcgcgttgc gatcgcgcgt
     2401 gcacttgtga ctcaacctgc cgtgatcctg gcagatgagc caacgggcaa tctggatagc
     2461 aagactggca aagagatcat gtcgcttttg ggtgagctgc atgccgaggg ccggaccatc
     2521 atcatcgtga ctcacgatat gaacattgcg cgccatgcca atcgaatctg tattatgcag
     2581 gatggaatcc tgatggaaga ggaatcgctg catgtcgctg gctgacctga ttcgtacttc
     2641 cttgcgcggc gtttcagcga ataagctacg cgccgctctc acgatgttgg gcatcatcat
     2701 cggcgtggca tcggtcattg cgatgctggc tctggggaat ggcgcccgcg ctgccgtgga
     2761 tgcacgcttt cgctttttgg gagccaacca ggtgcagatc agcgcgatga tggcaatcga
     2821 cgatgaacaa tttgtctctg cgggaaagaa tctcagctat gaagatggct tgctgatgcc
     2881 cggtgaagtt gagctggttg gcagtgtcga tatgtcgatc ggtgccgctg caaaggcccg
     2941 cttcggacgc gccacgctgg atatcaatat ttctgggatc accgccaacg cactggagag
     3001 aatcgtcagc gcgggcgaag tgcagccggt caatcgacag gatggcgagg cattgacgat
     3061 cgatagctat atcgccgga
//
LOCUS       DASTJP010000476         6091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571 k141_4010,
            whole genome shotgun sequence.
ACCESSION   DASTJP010000476 DASTJP010000000
VERSION     DASTJP010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4010"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFQ23_13765"
     CDS             complement(<1..408)
                     /locus_tag="VFQ23_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907714.1"
                     /translation="MANKFLKINQGLLAGILIYCAFVYILYLRMKSAEKLLVDYSVAR
                     LCILTSNCREIIKAEILDYGSSKISFANYGSRGIPLESGSFEEYLFTLSVKNSETRIL
                     KVLPNTPSNTNGFDVKNIYIPTKSDEYFLKGNIF"
     gene            complement(401..1588)
                     /locus_tag="VFQ23_13770"
     CDS             complement(401..1588)
                     /locus_tag="VFQ23_13770"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HET9907715.1"
                     /translation="MPINFQQVYTRIKEIADGADDTKRILEERRFQARIWLGEFGADL
                     DYLKYKVEQAKLVDANIRCAAPFKDPLTAIFPPPANVSDVTVIAADGSQINPDRHAAV
                     QFCIINVGVIGMRLSSGEAPDVSIETELLYGDDLLRNGSPLSDGMVAMRRDISERIKL
                     DEFSRDMTGPVVNLTDGTLELWGAKGDDPQAYANFVEQYLGVLSRLHSRGVVTAGYVE
                     KPTADLVVRLLEIASADTEQMQRIREYQPLRGVSDRWLYGEREDPILPPYHRSAVFVL
                     QSGSEKKYKGALALHFFYLNVGSEGHPWPVRVEIPQWVAEDNKKLDILHRVLIEQCKM
                     MGSRPYPYLLHRAHETAVVRYEEKGQVEQLIALELRRIRADVGDSSNKSSAKALKGKS
                     KYG"
     gene            2121..2852
                     /locus_tag="VFQ23_13775"
     CDS             2121..2852
                     /locus_tag="VFQ23_13775"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET9907716.1"
                     /translation="MNNKSIFVIGALLLALLVSACGGPTTINQAAAPAIRTVSVSGSG
                     QAFLEPDIAYIYLGVHTEKLTASEAVAENNSQTETMIQALRDFGIDAKDIRTTNFSIW
                     PSDKYDPATGLPTGEKTYMVDNTVYVTVRELDRLGDLLDTVVTAGANTVNSVQFDVAE
                     KDAALKDARAQAVKDAQAKAQELAEAAGLSLGEIHSIEFFDNQPYPLFEGKGGGGRDV
                     AAAAVPIQPGQLTFTVTVNVSYVLQ"
     gene            3063..3947
                     /locus_tag="VFQ23_13780"
     CDS             3063..3947
                     /locus_tag="VFQ23_13780"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9907717.1"
                     /translation="MLKVGYIGLGLMGKSIARNILKAGFPLVVHNRSRGAVDELVAEG
                     ATAASSPKEVASQVDMVFTNLPDTPDVEKVVLGENGMVEGAHAGLIYIDNSTIKPASA
                     RMLAEELSKKNIFALDAPVSGGDIGARNATLTIMVGGDASALEKAMPVLLAMGKTVTH
                     VGDSGAGQVAKAANQIMVAAQMVAMGELLVFSKKAGVDPRKVVDAIKGGAAQCWTLDV
                     KPPRLFEGNRSPGFKAHMQLKDLGIILDTAKEYDIPVSATIENTKLFQEMIDQGMGEL
                     DNSAVVGVMEKEAGVHIV"
     gene            4097..4987
                     /locus_tag="VFQ23_13785"
     CDS             4097..4987
                     /locus_tag="VFQ23_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET9907718.1"
                     /translation="MKLYWPLIRDAVLIVLAAFIQALSLRLFFIPSKLAFGGVSAIAQ
                     LINHFTDWPIGLMIFIGNVPLFALGWRYLGGRAFVIRTAIAIFTYSMLTDLLLQTPLF
                     APNGPATRLINDLQGDIFLNALYGAIVSGIGYGLVYRARGTSGGSDILARILNNWRGI
                     PITQSYLIVDTAVILGAGIVFGWKEALYALIALYVSGLVAETTLEGGGTIRTAMIITS
                     EADAVSQRVLEELERGVTVLEGVGAYTGADRPVLYCVVTRAEVATLKAIVHGVDPHAF
                     MVIGTAHEALGAGFKPLKER"
     gene            complement(5029..5952)
                     /gene="era"
                     /locus_tag="VFQ23_13790"
     CDS             complement(5029..5952)
                     /gene="era"
                     /locus_tag="VFQ23_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET9907719.1"
                     /translation="MNDFRSGYIAIIGRPNVGKSTLVNALLGQKVAAVSPKPQTTRKR
                     QLGILTADSYQLVFVDTPGIHMAKHKLGQFLNQEAQASLEGVDAILWLVDVSVKPTEE
                     DKQIGSLLNSIPRRTPLVLAANKMDLVPAEALTARLEAYQASLKKEARLIFISAAQNQ
                     NLDQLLELLILLSPIRPPEFDEEQVTDSYERDIAADLIREACLHHLRDEVPHGVGVRV
                     DEFAERDNGMLYIAATIFVERNSQKGILIGEGGRMMKAIGSGARKEIEEMSGRKVFLE
                     LRAKVLKDWRNDENAMQRLGYRIKNKKKSRR"
BASE COUNT         1426 a         1559 c         1577 g         1529 t
ORIGIN      
        1 aaatatattt cccttcagga agtattcatc tgactttgtt ggaatataga tgttctttac
       61 atcaaagcca ttagtatttg atggagtatt aggtagtacc tttaagattc tggtttctga
      121 attttttact gatagggtaa ataagtattc ttcaaaactc ccactttcca gtggtatgcc
      181 tctggatcca tagttggcaa aagatatctt gcttgatcca taatccaaaa tctctgcctt
      241 gataatttca cgacaatttg aagttaatat acataaccga gccacggaat aatcaacaag
      301 caatttttcc gcactcttca tgcgcaagta aagaatgtaa acgaaagcac aatagattaa
      361 gattcctgcc agcaaaccct ggttgatttt gagaaatttg ttagccatac ttggatttcc
      421 ccttcaatgc ttttgcagaa ctcttgtttg aactatcacc cacatctgca cggatgcggc
      481 gtaattccag ggcgatcaac tgttccactt gtcccttttc ttcatatctc actaccgcgg
      541 tctcgtgggc gcggtgcaga agataaggat agggtctgct ccccatcatt ttgcattgtt
      601 cgatcaggac gcgatggagg atatccagct tcttattgtc ttctgccacc cattggggga
      661 tttccacgcg tacgggccag ggatgtccct cggagccaac gttcaaatag aagaagtgca
      721 atgcgagcgc gcctttatat ttcttctcgg agccggactg aagcacgaac accgccgaac
      781 gatgataggg aggcaggatt ggatcctcac gttcgccgta caaccatcgg tcgcttacac
      841 cgcgcaaggg ttggtactcg cgtattctct gcatttgttc cgtatccgcg ctggcaattt
      901 ccagcaaacg cacaaccaga tctgcagttg gcttttctac ataaccagcg gtgacaacgc
      961 cgcgtgagtg caaccgcgac aggactccca gatattgctc gacgaaattt gcataggctt
     1021 gcgggtcatc ccccttggct ccccataatt ccaacgtccc atcggtgagg ttgactacag
     1081 gtccggtcat gtcccgtgag aattcgtcaa gcttgattcg ctcactgatg tcacgacgca
     1141 tggcgaccat gccgtctgat aagggacttc cgtttcgtaa gagatcatcc ccatacagca
     1201 gctcagtttc gatagacacg tcaggtgcct ccccggaact cagcctcatc ccaatcaccc
     1261 ccacgttgat gatgcaaaac tggaccgccg cgtgccggtc cgggttgatc tgcgaaccat
     1321 cggcagcgat gacggtcacg tcactaacat tggcgggtgg ggggaagatg gcggtgagcg
     1381 gatccttaaa cggggcggca cagcggatat tcgcgtccac gagcttggcc tgttccacct
     1441 tgtatttcag gtaatccaga tcagccccaa attcgcccag ccagatgcgc gcctgaaagc
     1501 gccgttcttc cagaatccgt tttgtgtcat cggctccgtc ggcgatctct ttgatgcggg
     1561 tgtagacttg ctgaaaattt atcggcatat tagctacctg tcacacctgc atgatttgct
     1621 tagcaaatca cgtgcggcgc aagtgttgcg agccttgcaa agcaaggcgt ggcaatctcc
     1681 ttattacgag gagattgctt cgtcgggcta aggccctcct cgcaatgaca taaatggtta
     1741 gaacaaatgt accaagattg tacagggaat cagtccaaaa tacaagactt ttagcaccac
     1801 atctgggcat tgaaatgctt tggatactct atttaggcat aggaataggg ggaattttct
     1861 cagtttttgg agaaaactaa ccacctggat attgtttcaa aaacaaaccg ctggtcgagt
     1921 aggcggatgc tttcgcgaag cggccgtatc gagaccaaca gtatcttaca atcagaagat
     1981 tttctgcaaa tctttggtct cgatataccc ctcggcttac ctctggggct actcgaccca
     2041 cggaatttgc tgctatgcgg gaacttttca gtatgatggg cgtctatcgt gtagtagatt
     2101 attgcacaag gagcttatga atgaacaaca aatctatttt cgttatcggc gcgttactgc
     2161 tggcattgct ggtaagtgca tgcggtggac cgacgaccat taatcaggcg gctgcgcccg
     2221 ccatccgcac cgttagtgtt tcaggttcgg gccaggcatt cctggaaccc gatattgcct
     2281 acatctatct gggcgtgcat accgagaagc tgactgcttc cgaggctgtc gcggaaaaca
     2341 attctcagac cgagaccatg atccaggcgt tgcgcgactt tggcatcgat gccaaggata
     2401 tccgcaccac caacttcagc atctggccga gtgacaagta tgacccggcg accggcttac
     2461 ccaccggcga gaagacgtat atggtggata acaccgttta tgtgacggtg cgcgaactgg
     2521 atcggcttgg cgacctgttg gacacggtcg ttacggctgg agcaaatacg gtcaacagtg
     2581 tccaattcga tgtggctgaa aaggacgctg ccctcaagga tgcccgtgcc caggctgtga
     2641 aagatgccca ggcaaaagca caggaactag ccgaagcagc cggactctca cttggtgaaa
     2701 tccactccat cgagtttttc gataaccagc cgtacccact ctttgaaggc aagggcggtg
     2761 gtggcagaga tgtagctgca gcggcggtgc cgatccagcc cggacaactg accttcaccg
     2821 tgacagtgaa cgtctcgtat gttctccaat aattagagca ctcttctcct cagattggac
     2881 ctgcttccct aaaggaagca ggtcctttca ttatgacccc caccgtcctt tggacacctc
     2941 cctcaaatac gccaatgata gatgcttgat gattgtcaaa acttttgtgg cggatttggg
     3001 ggaggctggg aggggggcgc gttataatcc ccaaatcctc ttcacgttcc ataggagtca
     3061 ttatgctcaa agtcgggtat atcgggttgg gtctcatggg caagtctatt gctcgcaata
     3121 tcctcaaggc cgggtttccg ctggtcgtac acaatcgttc gcgcggcgca gtagatgaat
     3181 tggtagctga gggagcaact gccgcgagtt ctccgaaaga agtggcttct caagtggata
     3241 tggtctttac caacctaccc gatactcccg atgttgaaaa agtggtgctt ggcgaaaacg
     3301 gaatggttga gggcgcgcat gctggcctca tttatattga caactccacg atcaagcccg
     3361 cctcggcgcg gatgctcgcg gaagaattat cgaagaagaa tatatttgca ctagatgctc
     3421 ccgtctctgg tggggatatc ggcgcgcgca atgcgactct caccatcatg gtcggcgggg
     3481 acgcttcggc gcttgaaaag gcgatgcccg ttctactggc aatgggaaag acggtcacgc
     3541 acgtgggcga ctcaggtgca gggcaggttg ccaaagccgc caatcagatc atggtcgctg
     3601 cgcagatggt agcgatgggt gaactgctgg ttttctcgaa gaaggctggt gtcgatccac
     3661 gcaaggtggt ggatgcaatc aagggtggcg cggctcaatg ctggactctg gatgtcaagc
     3721 cgccgcgtct gtttgaaggg aatcgcagcc cgggcttcaa ggcacatatg caattgaagg
     3781 atctgggaat catcctggat accgcgaaag aatatgacat tcctgtctca gcaaccatag
     3841 aaaacacaaa actcttccag gagatgatcg atcaaggtat gggcgaactg gataattcgg
     3901 cagttgtagg tgtgatggag aaagaggcgg gggtacacat tgtataaaaa aaccgttggt
     3961 cgagtaggac gagcgcaatt ctcgtccata tcgagaccaa cagttacaag caaacaaaaa
     4021 ctatcttgaa aatgtggtgg tctcgatacg tcctctaaaa gtattcggac tactcgacca
     4081 cctgatggtt caaactatga aactatactg gcccctcatc cgtgatgcag ttctgattgt
     4141 tcttgccgca tttatacagg ctctatcttt gcggttattt tttattccgt ccaagctggc
     4201 gtttggcggg gtgagcgcga tcgcgcagtt gatcaatcat tttaccgact ggccgattgg
     4261 cttgatgatc ttcataggga acgtgccatt gtttgctttg ggctggcgtt atctcggcgg
     4321 gagggcgttt gttatacgga cagccattgc tatctttact tattccatgc tgaccgacct
     4381 cttgctgcaa acacctctct tcgccccgaa cggcccagca acccggctta tcaatgatct
     4441 tcagggcgac atttttctga atgccctcta tggagcgatc gtcagcggta tcggctatgg
     4501 actggtctat cgggcgcgcg gcaccagcgg cggttcagat attctggcgc gtatcctaaa
     4561 taactggcgg ggcattccca tcacgcaaag ttatttaatc gttgatacgg cagttatttt
     4621 gggagcggga atcgtctttg gctggaagga agccttatat gcactgatcg ccctctacgt
     4681 gagcggcctg gtcgcggaaa cgacactgga agggggtggg acaatccgca cggcgatgat
     4741 tatcaccagt gaagccgatg ccgtttcaca acgcgtgctc gaagaactgg aacgcggtgt
     4801 gaccgtgctc gaaggggtag gtgcctatac aggagcagat cggcctgtgt tgtattgtgt
     4861 tgttaccaga gcagaggtcg caaccctgaa ggcaatcgtc catggagttg acccgcatgc
     4921 gttcatggtt atcggtacag cacatgaggc attgggtgcg gggttcaagc ctcttaagga
     4981 aaggtaaatt acttgtaggg gcgagccccg ggctgggtta ccctgcccct accgacgcga
     5041 ttttttctta ttctttatcc gataccccaa tcgctgcatc gcattctcgt cattgcgcca
     5101 gtctttcaga acctttgcgc gtaattcgag aaacaccttt cgcccgctca tctcttcgat
     5161 ctctttgcgc gccccggaac caatcgcctt catcatcctg ccgccttcgc cgatcaaaat
     5221 ccctttttgc gaattacgct ccacaaagat cgttgctgca atgtaaagca tcccgttgtc
     5281 gcgctcggcg aattcatcca cgcgcacgcc gactccatgc ggcacttcat cccgtagatg
     5341 atgcagacat gcctcacgga tcaggtccgc ggcgatgtcg cgctcgtagg aatctgtgac
     5401 ctgctcttcg tcgaattcag gcgggcggat gggactgagc aatatcaaca attccaacaa
     5461 ttgatccagg ttttgattct gcgctgcaga gatgaatatc agcctcgcct cttttttaag
     5521 cgacgcctgg tacgcttcta gacgcgcggt caacgcctca gcggggacca ggtccatttt
     5581 gttcgcggca agcacgagcg gagtccgacg cgggatgctg tttaaaaggg acccgatctg
     5641 cttgtcctcc tcggtcggct taacggaaac atccaccagc cacaggatcg catccacgcc
     5701 ttccagtgat gcctgtgctt cctggtttag gaactgcccc agtttatgtt ttgccatgtg
     5761 aatacccggc gtatccacaa acaccagttg atacgaatct gcagtgagaa tcccaagctg
     5821 acgtttgcgc gtggtctgcg gtttgggcga gacggctgcc actttttgcc ccagcagcgc
     5881 attgaccagt gttgacttgc ccacattggg tcgaccaatg atggcgatat aacctgaacg
     5941 aaaatcgttc atcgaatcct ttcataaatg cgaatcgctg gattcacgaa ggtgatgttc
     6001 atgcgcggaa ggtagggcgg cagcgtatag gaaaactctg ctaccagttg gtagtgatcg
     6061 gatcgtcctg tttccaactg cctgaaaaaa t
//
LOCUS       DASTJP010000477         4107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1044193, whole genome shotgun sequence.
ACCESSION   DASTJP010000477 DASTJP010000000
VERSION     DASTJP010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044193"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFQ23_13795"
     CDS             <1..554
                     /locus_tag="VFQ23_13795"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9907720.1"
                     /translation="LPFHTPLPDKQNPEVALFSRYPILEAEQLNLPFSDRSWKTIVDL
                     GEVNINVIVLHLTPTRAEEVPMSQWTTRITERQIVRMEQVERVIESVRNSPEPDLVLC
                     DCNFTEFSDAYARLDEFLDDGFARAGWGFGHTIHPVGIDIRLQRVDYVWYSSGFNAIS
                     AKVIKDGKSDHNPLVVELDLLLE"
     gene            610..1611
                     /locus_tag="VFQ23_13800"
     CDS             610..1611
                     /locus_tag="VFQ23_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907721.1"
                     /translation="MTTHSTSSVIRWLLDSDPSIRWQVIQDLLDTSDEDVAVERAKVA
                     TEGWGAKLLSLQGEDGSWAGVAWNHGWDSTMHVLMLLRDMGLDPASEQAQRAVDLVRD
                     HVHWQGWDWDGTWRGMDFVGNTFFAGEVEPCINGQVGAAGAYFGQDTQRIIDRLMNEQ
                     LPDGGWNCEAENGSTRSSFNTTICVLEALLEYELAGGANSDVMNARLRGQEYLLERRM
                     FRRLSTGEVIERDRKGETVWSRFAFPTWWHYDVLRGLEYMRRARVVPDERVTEAIEMV
                     ESRRDREGRWHLDVQYPGQMPVEINEGEGQPSRWITLRALRVLDWYDSRHKTNTDDS"
     gene            1640..2125
                     /locus_tag="VFQ23_13805"
     CDS             1640..2125
                     /locus_tag="VFQ23_13805"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9907722.1"
                     /translation="MSKFKAKIEIIGINPFVFLPERVLQKVFKQSGKDKGKIPVRMKI
                     DGHEFPQTLIKWSGAWRLYLNTPMRKAAKKDVGDIANFEIAYDSRERVFPMHPKFEKA
                     LRENKEAKKIFDSLRPSLQLEINRYLDSLKTESSVDRNVTKAIQFLLGKERFVASDHP
                     K"
     gene            2211..3050
                     /locus_tag="VFQ23_13810"
     CDS             2211..3050
                     /locus_tag="VFQ23_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET9907723.1"
                     /translation="MDLSICSYSFHRMLEEGQQDVFKFISDCQSLGCTQLDLWNGHLP
                     SLLDDATRSASSFSPDYAQLSADELDYLSRIKAAADDASLPFGCLAVDGAHIYEPTVE
                     ARQAQQIKAYRWLNIAEKLGTQQIRIDSGGTAEMPEEVFDIIVSGYNDLIPRAGEKGM
                     EIVIENHWGASHIPANVIKILDAVEGLGLLFDTGNWPKELRETGWTECARYARATHIK
                     TFAFDAEGNETTVDIPRAMRMLQETGYQGSWGIESVPREGDEIEAARKSVALVRRVLG
                     ESK"
     gene            3072..>4107
                     /locus_tag="VFQ23_13815"
     CDS             3072..>4107
                     /locus_tag="VFQ23_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9907724.1"
                     /translation="MNSSNHRPVRIGLIGAGQRGGHHLENYQQIPGAEIVAIADINET
                     LTKRVAAQYNIPNVYTDYHELLQRDDLDAVDICLHNNLHRPVAVAAMEAGKHVYCEKP
                     MAGSYADAKIMYDTAQSLGRKLQIQLFTLFRPETRAAKLLIDEGHLGELYHARSTGFR
                     RRGRPYVDGYGTPTFVQKRHSAGGALYDMGVYHIAGLLYLLGNPSVARITGKTYQKTP
                     MDSDREKDSGYDVEELGLGFVHFANDVTLDIIEAWAVHLDNIEGSSILGTLGGIRLEP
                     FGFFHNIGDLEINSTAEVDKIPYRWQNVHFGGDVYNSSEHHWVAALQGRVDLLPSAEL
                     ALNTMLISEGI"
BASE COUNT         1037 a         1003 c         1125 g          942 t
ORIGIN      
        1 atctcccatt tcatacgcct ctcccagaca agcaaaatcc tgaggtcgcg ttatttagtc
       61 gatatccaat cctggaggca gagcagttaa acctcccgtt tagcgatcgt tcctggaaga
      121 ccattgttga ccttggtgaa gtaaatatca acgtcatcgt acttcatctt actccgaccc
      181 gagcagagga agtgccgatg agtcaatgga ccactcgaat tacggaacgt cagattgtgc
      241 gcatggagca agttgagcgc gtaattgagt ccgttcgcaa tagcccggaa cccgatttgg
      301 ttctatgcga ttgcaacttt acggagttct cagatgccta cgccagactg gacgaattcc
      361 tggatgatgg ttttgcacgg gccggctggg gatttggtca taccatccat ccagttggca
      421 tcgatattcg gctccagcgg gttgattacg tttggtattc ctcgggcttt aacgcgatat
      481 cggccaaggt catcaaagat gggaagtcgg atcataaccc attggtcgtt gagttggatc
      541 tcttgttaga ataataatca aggtcgttag actacagact gtgtaagtag aagtaaggaa
      601 aacagacaga tgaccactca ttcaactagc tccgtcatac gctggctcct tgattcggac
      661 ccatcgattc gctggcaagt gatacaggac ctgctcgata catcggatga agatgtcgcg
      721 gtcgagcggg caaaggtcgc cacagagggc tggggcgcga aactcctctc actccaaggg
      781 gaagacggct cgtgggcagg cgtggcctgg aaccacgggt gggactctac catgcatgtg
      841 ctgatgctcc tccgtgatat gggtctcgac cccgcgagcg aacaagccca acgcgcggta
      901 gatctcgttc gcgaccacgt gcactggcag ggctgggatt gggatggcac atggcgagga
      961 atggactttg ttggcaacac cttcttcgcg ggcgaggtgg agccttgtat caacgggcag
     1021 gtgggagcag ccggcgcgta cttcggtcag gatacgcagc gaatcattga ccggctcatg
     1081 aatgagcaac tgcctgatgg tggctggaac tgcgaagctg agaacggttc gacgcggtcg
     1141 tccttcaaca caacgatctg tgtccttgag gctcttctgg agtacgaact ggcaggcggc
     1201 gccaattcag atgtgatgaa tgcgcgtctg cgtgggcagg aatatctcct cgagcgtcgc
     1261 atgtttcgaa ggttatccac gggcgaggtg atcgagcgcg accgcaaagg cgaaactgtc
     1321 tggtctcgct ttgccttccc aacctggtgg cactatgatg tgctgcgcgg gctcgagtac
     1381 atgcgccgcg cccgcgttgt accagatgag cgagttaccg aggcgattga gatggtcgaa
     1441 tccaggcgcg accgggaagg ccgatggcac ctcgatgttc aatatcccgg tcagatgcca
     1501 gtcgaaatca acgagggtga aggccagccg agtcggtgga tcacactgcg cgccttacgc
     1561 gtgttggact ggtatgattc ccggcacaaa accaacactg acgacagtta agtagagaga
     1621 caagttgccg cccatggaca tgtctaaatt caaggccaaa atcgaaatca ttggcatcaa
     1681 tccttttgtg tttctccctg aaagggtatt gcaaaaagta ttcaagcaat ccgggaagga
     1741 caaaggaaaa atccctgtac gaatgaaaat cgacggacat gaatttccgc aaactctcat
     1801 aaaatggagt ggtgcctggc ggttgtatct caacacaccc atgcgaaaag ctgccaaaaa
     1861 agatgtaggg gatattgcaa actttgaaat tgcatatgat tctcgcgaga gagtctttcc
     1921 catgcacccg aaatttgaaa aggcactacg agaaaataag gaagcaaaaa aaatattcga
     1981 cagcctgcgc ccatctttac agctggagat caaccgatat ttagattctt taaaaaccga
     2041 atcttcggtg gatagaaatg tgaccaaagc cattcagttc ctattaggga aagaaaggtt
     2101 tgttgcaagt gatcatccca aataggttaa agtaagggat acaataattt tcacctcaag
     2161 gagacgtcac atgaccaaaa gcgagcaatc tcagaagcac ggagcaatac atggatctta
     2221 gtatttgttc ttacagtttt caccgaatgc tggaagaggg tcagcaggat gtcttcaagt
     2281 tcatcagtga ctgtcaaagc ctgggatgca ctcagcttga tttatggaac gggcatctac
     2341 cttcgttgct ggacgatgcg acacggtctg catccagctt ctcgccggac tatgcacagt
     2401 tatccgctga cgagttggat tatctctcga gaatcaaagc tgcggcggat gatgccagtt
     2461 tgccttttgg ctgtctggct gtggatgggg cacatatcta cgagccaacc gtggaggctc
     2521 ggcaggcgca gcagattaaa gcctatcgct ggttgaatat tgcagagaaa ttaggtacgc
     2581 agcagattcg aattgactcg ggtggtacgg ctgaaatgcc agaggaggtc tttgacatca
     2641 tcgtcagcgg atacaacgat ttgattccgc gcgctggcga aaaagggatg gaaatcgtca
     2701 tcgagaatca ttggggcgct tcacacatcc ctgcgaacgt cataaagatt ctggacgctg
     2761 ttgagggatt aggcttactg ttcgacaccg ggaattggcc caaagaactg cgtgaaacag
     2821 gctggacgga atgcgcccgc tatgctcgcg ccacccatat taagacgttt gcttttgacg
     2881 ctgagggcaa cgagacgacc gtggatatcc cgcgggcgat gcgtatgtta caggagacag
     2941 gttatcaggg cagttggggc attgagagcg ttcctcgtga aggcgatgaa attgaagccg
     3001 cccgaaagag cgtggctctt gttcgccgcg tattgggtga gtccaaataa tatagcagtg
     3061 gagcagatca gatgaattca tcaaatcacc gaccggttcg gatcgggttg atcggcgccg
     3121 ggcagcgagg cggacatcat ctcgaaaact atcaacagat tcccggcgct gagatcgtcg
     3181 ctatcgccga tatcaacgaa accctgacga agcgcgtcgc ggcccaatac aacattccta
     3241 atgtatatac cgattaccat gagctcttac agcgtgatga cctcgatgcg gtagatattt
     3301 gtctgcataa caatcttcat cggccggttg cagtcgcagc catggaagct gggaagcatg
     3361 tctattgtga aaaaccaatg gctggctcat atgctgatgc gaagatcatg tacgacacgg
     3421 cccagtctct tgggcgcaaa ctccagattc aactcttcac gttatttcgc cctgaaacgc
     3481 gcgccgcaaa actgctcatc gacgaaggtc acctggggga gctctatcac gcgcggtcaa
     3541 caggattccg tcggcgcggc cgaccatacg tggacggcta cggcactcct accttcgtgc
     3601 aaaagcgaca ttcagcaggc ggcgccttgt atgacatggg cgtctaccac atcgccggac
     3661 ttctttatct gctgggtaac ccgagcgtgg cgcgcatcac cggcaagacc tatcagaaga
     3721 ctccgatgga ttccgaccgt gaaaaagaca gcggttatga cgttgaagaa cttggcctgg
     3781 gtttcgtcca tttcgccaat gatgtcacgt tagacatcat cgaagcctgg gcggttcacc
     3841 tggataatat cgaaggctca agcattctcg gaacgctggg tggcattcgc ctcgaaccgt
     3901 ttggtttctt tcataacatt ggtgatcttg aaatcaacag tacggccgag gttgataaaa
     3961 ttccatatcg ttggcaaaac gtacacttcg gtggtgatgt ctataatagc tccgagcatc
     4021 actgggtggc tgcgttacag ggacgcgtgg accttctgcc gagtgccgaa ctggccctaa
     4081 acaccatgct catctctgaa ggcatct
//
LOCUS       DASTJP010000478         9542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1216350, whole genome shotgun sequence.
ACCESSION   DASTJP010000478 DASTJP010000000
VERSION     DASTJP010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216350"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFQ23_13820"
     CDS             complement(<1..529)
                     /locus_tag="VFQ23_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9907725.1"
                     /translation="MTIKNPPKISIEQVAELSANVVQEVEKAVVGKREVLNLMMAAFL
                     SAGGHILLEDYPGLAKTLIANSFAKALGMEFKRIQFTPDLLPGDITGGYVFDSEHNRF
                     TLRKGPIFANILLADEINRASPKTQSALLEAMQEYQVTLEGETLELAEPFIVIATQNP
                     IEYEGTFPLPEAQLDR"
     gene            complement(495..1166)
                     /locus_tag="VFQ23_13825"
     CDS             complement(495..1166)
                     /locus_tag="VFQ23_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907726.1"
                     /translation="MHRSQRFILVFLVAALVVFLGMLFWPFVLNNILQPTALTLWLLL
                     RILVLSIHQQYFWYTVILVAAVVLVRLLRETQPERPPQASFETNTALSIISDWRDLLL
                     YNGQEALEDKTLKRQLANLLTSLYALKQGASNDFRLYDDLQRGTIPLPENIHTFLFAP
                     EPASGGPLQKFFQSIQKAPRRWIRQWTGQAKAEHHKMIDDVLNFIETSLEINSDDKKP
                     SQNKH"
     gene            complement(1159..2031)
                     /locus_tag="VFQ23_13830"
     CDS             complement(1159..2031)
                     /locus_tag="VFQ23_13830"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HET9907727.1"
                     /translation="MTGSTKRKTIVLLGLVMLLTIVVAANLQQVEFQPGMPLPRLEQG
                     QLVVGAINDPSFSISAPSFILILIALVLTAATLYTAYQIFRGADWQLILSVLRFTLII
                     SVSISCLVFMVMLFPNSNIDTPAEITMPPIPPQPVVTSPVASAPAWLLWLVGAGLLII
                     TVLVALWSFRPVRQADQLDLLGLEAERARRALMSGAELKEVIINCYREMSLTMKQERG
                     IEREEFMTTREFETVMESVGLPPEPIHRLTRLFEAVRYGHWQPNATDEQQAIQSLEAI
                     MVNSRTVRAKNRNA"
     gene            complement(2256..5528)
                     /locus_tag="VFQ23_13835"
     CDS             complement(2256..5528)
                     /locus_tag="VFQ23_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907728.1"
                     /translation="MIKKLLLTLAICSGLVLGSLHPAPGRAFAQEEVPEATATEAPTE
                     LPPTLEPIATETALPPATETQVQAPTIQATATAMPSLPATVTTTSVPTFTATAITVQN
                     APDAIFADSFEAGNFSAWTSNSVDSGDLSISNAAALSGSRGIQAVIDDNNSLYIRDDS
                     PNAEPRYRARFYFDPNTIQMANGNKHFLFKGFVGTSTEVLQIEFSKTSTAYQMRAGLL
                     NDGSTWTYTTWFNVSDASHVIEFDWRAASGVGANNGGLTFWIDGAQKADLSGVDNDTR
                     RIDRIRLGVLGIDSGTRGTYYFDAFESRRQSYIGPAGNTAPQAPASTTIPPTVAVTVT
                     KTPTAVAATATKTSTVPALATNTSAPQVTSTATPVTGTSDTIFVDGFEAVNLTTWTSS
                     ITDPGRLSISPAAALVDRQGLQIVLNDNTSTFVTDETPSAEPRYRVRFYFNPNSIGMA
                     NDNAHFIFRGIMGTSTEVMRVEFRFSNGSYQLRAAAATDSSDRLNSNWFTITDSTHFI
                     ELDWRAASSGSASNGGLTFWLDGTQQADLVGIDNDTLRIDRARLGAVAGIDSGTRGTY
                     YFDAFESRRQSYIGASTGTTNPDTLPTVSVPTSTSVAPTSTSILPTSTTLPAITPGVV
                     QPPTGPVVAFPGAEGFGAQSLGGRGGRVIEVTNLNDSGPGSLRECVNAQGPRTCVFRI
                     GGTITTQSEIIVTNPYLTIAGHTAPGGGITLRAAQNYHEEPFIINTHDVIIRYVRFRA
                     GAAAVPGSSRRSLTISNGAYNIMIDHCSFSWATDQPLLLIDGVRNTTIQWSIVSEALA
                     NSTHMEEGVFQEHSTGLSVSGKNYRSTQRTGNITIHHNLLAHNGGRNPQNAGYGLEDV
                     VNNIIYNWGGQGFTTHDLQANVPSNIVANYFKAGVNSTGAEIRASHTNDANTGPQIYV
                     YGNIGPRRPSDTLPHINSVSSGDQIYVVPNRFPAPAVTTTSADVAYRNVLLKAGVRIP
                     MLDAVDKRILEEVKNGTGKIIDCVSANELTSPINCASRVYVSPADYTRYGISDPLDSK
                     GWPVLAAGTPPQDTDHDGMPDTWESARGLNPNTNDSAQDRNGDGYTNIEEYLYELAAA
                     FQ"
     gene            6056..7354
                     /locus_tag="VFQ23_13840"
     CDS             6056..7354
                     /locus_tag="VFQ23_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907729.1"
                     /translation="MKIKFVHGISLIAIMGFVLAACGPAETSTAMPATEMPHDMPTEM
                     ATEAPTEMPTEAPTEAATEMPVTTSDTGAADLRVALNTLLGEHVLLAASATNAALGGR
                     NDEFEAAAAALDTNSVDLSEAIESVYGADAGEAFLALWRSHIGFFVDYTTGVATQDQA
                     KQDKAVEGLTGYATDFGAFLSGANPNLTQDAVAGLVSEHVLTLKDVVDAQASGDATAA
                     FTSLRMAYGHMQMVADALSGAIVEQFPDKFDGQTDTGAADLRVALNLLLAEHTSLAAS
                     ATSAALGGRNDEFEAAAAALDTNSVDLSEAIESVYGADAGEAFLALWRSHIGFFVDYT
                     TGVATQDQAKQDKAVEDLTGYATDFGAFLSGANPNLTQDAVAGLVTEHVLTLKDVVDA
                     QASGDATAAFTSLRMAYGHMQMVADPLAEAIVQQFPENFQ"
     gene            7419..7832
                     /locus_tag="VFQ23_13845"
     CDS             7419..7832
                     /locus_tag="VFQ23_13845"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET9907730.1"
                     /translation="MFSSKRIYSAFITPLFAIMLAACGSAASNQGTATPVATTVSAAM
                     EPTSAEVQVEINLFNFSEDEITIPAATTVVWVNHDNIEHSVTSGTPENPDGAFDSDFF
                     VKDESFRMVFDTPGEYPYFCRRHNHMRGVIIVTAP"
     gene            complement(8094..8498)
                     /locus_tag="VFQ23_13850"
     CDS             complement(8094..8498)
                     /locus_tag="VFQ23_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9907731.1"
                     /translation="MIIRVALADDHPRVRSVLRLLLSMNKDIEFVCEANNGEEAVQCV
                     KQFEPDVLVMDVSMPIMDGFEATQQVTELEVSTQVILISFDIEEINVKKASEIGARGF
                     LPKDIVATELFPAIEAVSRGETFFKEKSDLTK"
     gene            9062..>9542
                     /locus_tag="VFQ23_13855"
     CDS             9062..>9542
                     /locus_tag="VFQ23_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907732.1"
                     /translation="MKKHWLNLCLIVFLMAGHNVQSVKADPTPVHTYVPIGSGYSAQT
                     LQLFARQAVQKDTDGIVELLVIPITFATDAFNISNGERQQNLTLAENRRGLVENACNA
                     VKLPGQTCRVVLAPVLVRADAFLQSNLDLFPADLDGMYILGGDQTVAMQVVANTPFEV
                     "
BASE COUNT         2185 a         2393 c         2586 g         2378 t
ORIGIN      
        1 agcgatccag ctgcgcttcc gggagcggga aggtgccttc gtattcaatc ggattctgag
       61 tggcgatcac gatgaacggt tccgccagtt caagggtttc gccttccagt gtcacctgat
      121 attcctgcat ggcttccagc aaagccgatt gggtctttgg cgaagctcgg ttgatttcat
      181 cggccagcag aatgttggca aagatcggac ctttgcgcag cgtgaagcgg ttatgctcac
      241 tgtcaaatac gtagccgcct gtgatgtcgc cgggcagcag gtcaggtgtg aactggatgc
      301 gcttgaactc catgcccagt gcctttgcaa acgagttggc gatcagggtc ttggccagac
      361 cgggatagtc ctccaacagg atgtgacctc cggctgaaag aaaggccgcc atcatgagat
      421 tgagtacctc gcgctttccg acaacggctt tttcaacctc ctgaactacg ttggcagata
      481 gttcggcgac ctgttcaatg cttattttgg gagggttttt tatcgtcact gttgatctcc
      541 agagatgttt ctatgaaatt gagtacgtca tcaatcattt tatgatgttc agcttttgcc
      601 tgccccgtcc actgtctgat ccatcgtcgg ggagcctttt gaatggactg aaaaaacttc
      661 tgtagcggtc cgcctgaggc tggctcgggc gcgaatagaa acgtatggat attctcgggc
      721 aaggggatcg tgccccgctg aagatcatca tacaggcgaa aatcattcga ggcaccttgt
      781 ttcaaggcat acaggctggt cagtagattg gcaagttgcc gtttcagggt tttgtcttcg
      841 agggcttcct gcccgttgta aagaaggagg tcgcgccagt cgctaatgat ggaaagggcg
      901 gtgttggtct cgaaggatgc ctgtggggga cgttcgggct gcgtctcacg gaggaggcgt
      961 accagaacaa ctgccgccac caaaataacg gtataccaaa aatattgctg atgaatgctc
     1021 aacaccagga ttcgtaacag aagccagagg gtaagcgctg tgggttgaag gatattgttc
     1081 aggacaaacg gccaaaaaag cattcccaga aaaacgacca gtgctgcaac gaggaacaca
     1141 aggataaaac gttgcgatct atgcatttct gttctttgcc ctgacggtgc ggctgttgac
     1201 catgatcgct tccagagact ggatggcctg ctgttcatcc gtggcattcg gctgccaatg
     1261 tccgtagcgg accgcctcaa aaagccgtgt caaacggtga atgggctccg ggggaagtcc
     1321 aacggactcc atcaccgttt caaattctct ggtggtcatg aactcttctc gctcgatccc
     1381 ccgttcctgc ttcatggtca ggctcatttc acggtagcag ttgatgatca cttccttcaa
     1441 ctccgctccg ctcatgagcg cccgccgggc cctttctgct tccagcccca gaaggtcaag
     1501 ctgatctgcc tggcgaacgg gtctgaaact ccacagtgcc acaagcacag taatgatcag
     1561 caaaccagcc ccgaccagcc agagcagcca tgcaggcgct gatgccacag gggaggtgac
     1621 caccggttgc ggcggtatgg gtggcattgt gatttccgca ggcgtgtcga tgtttgaatt
     1681 cgggaacaac atcaccatga aaacaaggca gctgatggac acactgataa tcagcgtgaa
     1741 gcgtagaaca gacaggatga gctgccaatc agccccgcgg aagatttgat acgccgtata
     1801 cagggttgcc gcggtgagta caagagcgat caagattaga ataaaacttg gtgccgaaat
     1861 agaaaaagac gggtcattga tcgcaccgac caccaactga ccctgttcca gccggggaag
     1921 cggcattcct ggctgaaact ccacctgttg cagattggct gcgaccacta tcgtgagcag
     1981 catcaccagc cccagtaaga caattgtctt gcgttttgta ctgcccgtca tctacaataa
     2041 gtctctcccg gctgttattg tacgaccaaa ctttatcaaa cgtaagaatg tgtttaccgt
     2101 gcatttgcaa atggagagga tttgagttca agtgccccct ccctaaccct cccccaaatc
     2161 ttttggattt aggggaggga actgaaacag acctcctgat accatatcag gaggtctgtt
     2221 ttattgttta tctagccggt tccggcttag gtatcttact ggaaagcggc tgcgagttcg
     2281 taaagatatt cttcgatgtt agtatatccg tcgccgttgc gatcctgggc actatcgttg
     2341 gtgttcgggt ttaagccacg ggcggactcc caggtatcgg gcatgccatc atggtcggta
     2401 tcctgcggcg gggttcccgc tgctaacacg ggccaaccct tgctatcgag cggatcacta
     2461 attccatacc tggtgtaatc agccggactc acataaaccc gcgaggcgca attgatcggg
     2521 ctggtcagtt cattggcgct gacacagtcg atgatcttgc ccgtgccgtt cttgacttct
     2581 tccaggattc gtttatctac ggcatccagc attgggatgc ggacgccagc tttgagtaac
     2641 acattccgat aagcaacatc ggccgaggtt gtcgtcacag ccggagccgg gaagcgattc
     2701 ggaacgacgt agatctgatc gccggagctc acgctgttga tatgcggcag ggtgtcggag
     2761 gggcggcgag ggccgatatt gccgtagacg tagatttgcg gaccggtatt cgcatcgttc
     2821 gtgtgacttg cccggatttc cgcccccgtt gaattgacgc ctgctttgaa gtaatttgct
     2881 acgatattgc tgggaacatt ggcctgtagg tcgtgggtgg tgaagccctg gccaccccag
     2941 ttgtaaatga tgttattgac aacatcttcc aaaccatagc cagcgttctg agggttgcgt
     3001 ccaccattgt gtgccagcag attgtgatga atcgtgatat tgccggtccg ttgcgttgac
     3061 cgatagttct tgcccgatac ggataagccg gtgctgtgtt cctgaaatac accttcttcc
     3121 atatgggtgc tattggcgag cgcctctgag acgatagacc attgaatcgt ggtgttgcgt
     3181 acgccatcga tcagcagcaa aggctggtcg gtcgcccaac tgaacgagca gtgatcgatc
     3241 atgatattgt aggcgccatt ggaaattgtc aaactgcgac ggctggaacc cggtacggcg
     3301 gcggcaccgg ctcggaagcg cacgtagcgg atgatcacat catgcgtatt gatgatgaaa
     3361 ggttcttcgt ggtaattctg tgcggcgcgc aacgtaattc cgccgcccgg tgcggtatgc
     3421 ccggcgatcg tcagataagg gtttgtaacg atgatctcgc tctgtgtggt gatcgtcccg
     3481 ccgatgcgga agacgcaggt gcgaggtccc tgagcattga cacactcccg cagactgccc
     3541 ggaccgctgt cattcagatt cgtgacttcg ataactctgc caccccgtcc tccgaggctc
     3601 tgcgccccaa acccctctgc gccagggaag gccactacgg gacctgtagg aggttgtacg
     3661 actcctggtg taatcgctgg gagcgtcgta gaggtgggca agattgatgt agaggtcgga
     3721 gcgactgaag tcgatgtcgg aacggaaacc gttgggaggg tatccggatt ggttgtgcca
     3781 gttgatgcac cgatatagct ttgtcgtcgt gactcgaagg catcaaagta ataggtgccg
     3841 cgcgtaccgc tatcgatccc tgcgacggca cccaggcggg cacgatcgat tcggagtgtg
     3901 tcattatcga taccaaccag atcggcctgt tgggttccat cgagccagaa ggttaagccg
     3961 ccgttgcttg cactcccgct ggaggctgcc cgccaatcca gttcgataaa gtgggttgaa
     4021 tcagtgatcg tgaaccagtt ggagttgagg cggtccgacg aatcagtggc tgcagctgcc
     4081 cgcaattgat agctcccgtt cgagaaacgg aattcgacgc gcatcacttc ggtggaggtg
     4141 cccatgatgc ctctgaagat gaaatgggca ttgtcattgg ccatgccgat cgagttcggg
     4201 ttgaaataaa agcgtacgcg gtagcgaggc tctgcgctgg gggtctcgtc ggtgacaaag
     4261 gtcgaagtgt tatcgttcag gacgatttgc agaccctgtc tatctacgag cgcggccgcc
     4321 gggctaatgc tgagccggcc cggatcggtg atgcttgagg tccatgtagt gagattcacg
     4381 gcttcaaagc catcgacgaa gatcgtgtca gaagtaccag tcaccggagt cgcggtagaa
     4441 gtcacctgag gcgcggaggt attggtggcg agggccggga cggttgatgt tttcgtggcg
     4501 gttgcagcca ctgcggttgg tgttttcgtg accgttacgg ctactgttgg tggaatcgtc
     4561 gtcgaggcgg gtgcttgagg cgcggtattc cctgccggac caatataact ctgacggcgc
     4621 gattcgaacg cgtcaaagta atacgtgccg cgcgtgccac tatcgatacc gagaacgccc
     4681 agccgaatgc ggtcgatacg gcgggtgtcg ttgtccacgc ctgagaggtc agctttttgc
     4741 gctccatcaa tccagaaggt cagtccaccg ttgtttgcac caacgccaga ggctgcgcgc
     4801 cagtcaaact cgatcacatg ggaggcatca ctgacattaa accaggttgt gtacgtccag
     4861 gtggagccgt cgttgagcag accggctctc atctgatacg ctgtggatgt tttgctaaat
     4921 tctatctgca gcacctctgt acttgtaccc acaaagcctt tgaagaggaa gtgcttgttt
     4981 ccgttggcca tctggatggt gttcgggtcg aagtaaaaac gcgcccggta gcgcggctcg
     5041 gcattggggc tatcatcgcg aatatagagc gaattgttgt cgtcgatgac agcctggatg
     5101 ccccggctgc cggacagagc tgctgcattg ctgatgctga gatcgccact atcgaccgag
     5161 ttggacgtcc aggcagagaa attgccagcc tcgaaactgt cggcaaagat cgcatctgga
     5221 gcattttgca ccgtgatagc agtagcagtg aatgtgggga ccgaggtggt tgtgaccgtg
     5281 gctggcaacg aaggcatggc cgtggccgta gcctggattg tgggtgcctg cacctgcgtc
     5341 tcggtggctg gaggcaacgc ggtctccgtt gcgatgggct ccagtgtagg tggtagttcg
     5401 gtaggggctt cggttgcagt ggcttcgggg acttcttctt gcgcgaacgc acgcccgggt
     5461 gcgggatgca ggcttcccag cactaagccg ctacagattg cgagcgttaa taagagtttt
     5521 ttgatcatgg ttctaccttc ttcttctttg attttttctt gcagtgtgat caaacagcct
     5581 tttctgtttg ctttctctcg ttgtgtctgg aggagagagt ggacatcatt cgagtccccg
     5641 ccgtagtgcg cctaagatat tattcggcgc tagcacatct caccagcgtt cgttacagtt
     5701 tttccctttc agaacagtta gctttccatg gattaacaaa atagaaagtg gtttacggga
     5761 cgcaaaaaaa agacctgcca tttggcaagt ctttttttgt ttattcaata aaaacggagg
     5821 tcaattcacg agtccttttt tagtaccacc accgccgccg gtgtgcggtc gttgacgccc
     5881 agtttgatga ataaactgct tacgtaattg catcgtcccc tccaaaatga atcattttta
     5941 atagtacagg tggcaaggat gtgctctgcc ccttgaatta gcgtttgctc attttcgcaa
     6001 atattcagat gtgaagaaat cgaagcaccg tagttagata tgaaggagaa agaaaatgaa
     6061 aatcaaattt gtacatggaa taagtctaat tgctattatg gggtttgtct tagctgcttg
     6121 tggacctgcg gaaacttcca ccgcaatgcc cgcaactgaa atgccgcatg acatgccaac
     6181 cgaaatggcc acggaggcac cgaccgagat gccgacagaa gcgccgactg aagcagcgac
     6241 ggaaatgccc gtaaccactt cagatactgg tgcggcggac ctgcgcgtcg ctttgaacac
     6301 attgcttggc gaacacgtcc tgctggcagc gagtgcaaca aatgctgcgt tgggtggacg
     6361 gaacgatgaa tttgaagcgg ctgcggctgc tctggatacc aactcggttg atctctcgga
     6421 ggcgattgag tctgtttatg gcgcggatgc aggtgaggcc tttttagctc tctggcgatc
     6481 tcacattggt ttctttgtag attacaccac gggtgttgct acacaggatc aagccaaaca
     6541 ggataaagca gttgaaggtc tgaccggcta tgccactgac ttcggtgcct tcctcagtgg
     6601 tgccaacccg aacctcactc aggatgcagt ggcgggtctg gtaagcgagc atgtgctgac
     6661 gttgaaggat gttgtcgacg cacaagcaag tggtgatgca acggccgcct tcacatccct
     6721 acgcatggcg tatggtcaca tgcaaatggt ggccgatgca ttgtctggag ccattgtcga
     6781 acagttccct gataagttcg acgggcagac ggataccggc gcggcagatt tacgtgtcgc
     6841 attgaacctg ctgctggctg aacatacctc tcttgcggcg agtgcaacaa gtgctgcgct
     6901 gggtggacgg aacgatgaat ttgaagcggc tgcggctgct ttggatacca actcggttga
     6961 cctctcagag gcgattgagt cagtctatgg cgcggatgca ggtgaggcct tcctggctct
     7021 gtggcggtct cacatcggtt tctttgtaga ttacaccacc ggtgttgcta cacaagatca
     7081 agccaaacag gataaagcag tcgaagatct cacgggctat gccactgact ttggtgcctt
     7141 cctcagcggt gccaacccga acctcactca ggatgcagtg gcgggcctgg tgacggagca
     7201 tgtgctgacg ttgaaggatg tagtggatgc acaggcgagt ggtgatgcaa ctgcagcctt
     7261 cacatccctg cgtatggcgt atggtcacat gcaaatggtg gctgacccat tggctgaagc
     7321 gatcgtccaa caattccctg agaacttcca ataagctgtt ttgaagattg aacaagcctg
     7381 ggtgaccagg cttgttcaat ttacaggtag aggtatttat gttttcaagc aagcgaattt
     7441 atagcgcgtt tattactccc ttatttgcga tcatgctcgc agcctgcgga agtgcggcaa
     7501 gcaatcaggg gactgcaact ccggttgcca caactgtatc tgctgcaatg gagcccacct
     7561 ccgcggaggt ccaggtggaa atcaatctct tcaattttag cgaagatgag atcacgatcc
     7621 ctgccgccac aacggtagtc tgggtaaacc acgataacat tgagcattct gttaccagcg
     7681 gcacgccgga gaatccggat ggcgcatttg actcggactt tttcgtcaag gacgagtcgt
     7741 tccgtatggt gttcgacacg cccggcgaat atccttattt ctgccgacgg cataaccata
     7801 tgagaggtgt aatcattgtg accgcgccgt aaggcaatag tgtaaaaaca attcgacaat
     7861 tcttattctc gacgaattgt cttttgactc ggttgacctc atttcactgg ctcggccagg
     7921 aactcttcgg accgaaccat acttaatcgc acatcccccc ggctcatgca ccgaggggat
     7981 gtgctgcata ccgcggatgg attcctgcgg attgctatct ctatcttaca tcgtttgcac
     8041 ggagcgaacg ttgcatgata gttacactaa acgattcaaa acaaatccat acgctatttc
     8101 gtcaagtcac tcttctcttt gaaaaaggtc tcgcctcgac taacagcttc aatggccggg
     8161 aataactccg tcgccacgat gtcttttggt aaaaatcccc tggcccctat ttcactggcc
     8221 tttttgacat tgatttcttc aatgtcaaac gagatcaaaa ttacctgtgt ggaaacctct
     8281 aattcggtaa cctgctgtgt agcttcaaaa ccatccatga tgggcatgct gacatccatc
     8341 acaagcacat ctggctcgaa ttgtttaacg cattgcacag cctcttcacc gttgtttgct
     8401 tcacaaacaa attctatatc tttgttcatg ctcaacaaca gtcgaaggac agatcgaact
     8461 cgtggatgat catccgctag agcaacacgt attatcatga tattgtcatc aaaaaatctt
     8521 ttctatctgc ttaccggaga cccccatcga taagttacta caattggtgc taattgattt
     8581 taatgaatgt cgagaaacct gtctattagg ctacttaccc attccctctg ggtaggttgc
     8641 cccatagcca ctttggagaa ataaagggtg attttcaaag cagcctgcct gtgctgagcc
     8701 caaccctgac cattgacatt ccgcaggaaa tcgattttct ccatacgccg cccattatct
     8761 gctacgttcg gagctttcga tttcgtgact ttaagctgta ctatgtttag ggtactaaaa
     8821 aatcttaaca gacgtataaa aaactgttaa acatgaaaag atgttaataa gttacatttg
     8881 gcttgccatt ttatagagag tcacataaaa tggacagcct ctggggaaac ttatggttga
     8941 tcaatagcac gtaagcgcac ttgaggaggt gcctcgcgtt cgtgaaaacc aaacttcaca
     9001 ccaagcttat gccgaaatgg cgtgttttca ctatcttcgc ttatctcccg aaggacaata
     9061 gatgaaaaag cattggctca atttgtgttt aatcgtattc ctaatggcag gtcataacgt
     9121 tcaatctgtt aaggcagatc caactccagt acatacctat gtcccgatcg gcagcggtta
     9181 ttccgcccag acgttacaac tcttcgcgcg ccaggcagtt caaaaagata ctgacggcat
     9241 tgttgagctc ctcgttattc ccattacgtt tgcgactgat gcattcaata tctcaaacgg
     9301 cgaacgccag cagaatctga cgctcgccga aaatcgccgc gggttagtag agaatgcctg
     9361 caacgcagtc aaacttcccg gtcagacctg ccgcgtggtt ctcgccccgg tcctcgtccg
     9421 tgcggatgcg tttttgcaga gcaatctcga tcttttccct gctgacctgg atggcatgta
     9481 catccttggc ggtgaccaga ccgttgcgat gcaggtcgtt gccaacacgc catttgaagt
     9541 ca
//
LOCUS       DASTJP010000479         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1016478, whole genome shotgun sequence.
ACCESSION   DASTJP010000479 DASTJP010000000
VERSION     DASTJP010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016478"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VFQ23_13860"
     CDS             complement(<1..1066)
                     /locus_tag="VFQ23_13860"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET9907733.1"
                     /translation="MKDSGKRYIGLDVHKYYLIAIGVDDELNVVLPARRVELSHLDSW
                     MKKTLTKRDAVVLEMTTNTWQIYDELCAYAGSVLVVHPPHVALITRSQVMNDKIAASI
                     LARLLAKGLLVGVWVPPQDIRELRGLVTQRKKMTGLATQAKNRLHAVLQRHHLLPPEG
                     NPFHQSQQDWWLALPLGTLEKVNVESDLETLRFAQRQQNRLTSIMEEIANEQEQVTRL
                     LQLPGFGVVTAVTVWAAIGDIQRFADPQHLVGYAGFGTRVHDSGMTSRSGRITKAGRR
                     DLRVVLIEAAQVAANSHPHWKAELARLQPRLGRNTPALAGGAREAIVAIARKLLVAVW
                     YILAQHKTDRFAQPEAIAQKF"
     gene            complement(1368..1508)
                     /locus_tag="VFQ23_13865"
     CDS             complement(1368..1508)
                     /locus_tag="VFQ23_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907734.1"
                     /translation="MPNGLRYRLVGGTRQRDFDGTNFKPRKLLENAHAAKQQSHQSGV
                     SM"
     gene            1665..2825
                     /locus_tag="VFQ23_13870"
     CDS             1665..2825
                     /locus_tag="VFQ23_13870"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HET9907735.1"
                     /translation="MTQQGFLIISDITGYSKYVNESELEHARDSLTALLNILIDHTQS
                     PLVLSKLEGDAVFSYAPAGGFLQGQSLLDMIESTYASFRKALELMVINTTCTCNACRN
                     LPNLDLKFFVHFGSYMTQKLGTFTELVGNDVNLVHRLAKNHIKEKTGFKAYAAFTQSV
                     MDALGLVEFQNSLTPHRETFADVGEVQMYVHDMHKVWERLKDRVRIEVRPEAAMVVIK
                     YEFPVPPSLLWEYVTKPEYRVILLGASKLELRNQAQGRTGVDSVFYCYHGNMETPQLI
                     LDWSPFEQFTTDDANPWGLTTRITCKLESTQAGTRLIMLMGKPRGTRLRVMLFSSIAK
                     LVFVPQMGKAAETLHQRILQDLADGTAFVSPPIEVDKAQITSLTTQALTHGH"
     gene            3155..3556
                     /locus_tag="VFQ23_13875"
     CDS             3155..3556
                     /locus_tag="VFQ23_13875"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9907736.1"
                     /translation="MSNRNVVHTEIPTADSNKVDSWFERLDHPLKDALTEVRKIILAV
                     DPRIKESIKWGTPTFSFNGDIVSIQSNAKKFVSLMFHRGAEIPGKHEGLLGTARLVRT
                     MKFIDLSEVKRRRKELESVIKAWCRWKSTGE"
BASE COUNT          866 a          958 c          950 g          856 t
ORIGIN      
        1 agaacttctg ggcaatcgct tcgggttggg caaagcgatc cgtcttgtgc tgcgccagaa
       61 tgtaccagac cgccaccagg agcttgcgcg caatagcaac aatagcttcc ctggcaccgc
      121 ccgccagggc aggtgtgttg cgtccgaggc gaggctgcag gcgagccagt tctgccttcc
      181 agtgaggatg cgagttggct gcgacctgtg cggcctcgat caggaccaca cgcagatccc
      241 gtcgtcccgc tttggtaatg cgtccactgc ggctggtcat gccactgtca tgaactcggg
      301 tcccaaaacc agcatagccc accaggtgtt gtggatcggc aaagcgctga atatcgccaa
      361 tcgcagccca gacggtcact gccgttacga ccccaaaacc aggtaattgc agcaaacggg
      421 tgacctgctc ttgctcgtta gcgatctctt ccatgatgga ggtcagacgg ttttgttgtc
      481 tttgtgcaaa ccgcagagtc tccagatcac tttcgacatt caccttttcc agagttccca
      541 gaggcagagc cagccaccaa tcctgttggg attgatggaa gggattccct tccggtggca
      601 ggagatgatg tctctgcaat actgcatgca ggcggttctt cgcctgggtg gccaatcccg
      661 tcatcttttt gcgttgggtc accaagccgc gcagttcacg aatgtcctgc ggaggcaccc
      721 acactcccac cagcaatccc tttgccagta agcgtgccag gatcgacgcg gcgatcttgt
      781 cgttcataac ttgagatctg gtgatcaagg ctacgtgtgg tggatggacc accaacaccg
      841 aaccggcata cgcacacaac tcatcgtaga tctgccaggt gttggtcgtc atttcgagca
      901 ccacagcgtc ccgcttcgta agtgtcttct tcatccatga atcgaggtgg gacaattcca
      961 cccggcgagc aggcagaacg acattcaatt cgtcgtccac accgatggcg atgagatagt
     1021 acttgtgcac gtccaatcca atataccgtt ttccagaatc tttcatgggg gtatactcct
     1081 gctgaaactg gcggacctcg ccaggagaag gccgggcacg tttctttttg gggccactgt
     1141 ttcactcaca gatgcgggct gtggcgcttc ttcgccattc ccagtttagc aaatgcgaac
     1201 ggggcagcca aacacccgaa cgagctttgg cgctcaaacg ccattctcaa gcacgtctcg
     1261 gctggaccct cacgcagcgt tcccggatac gcgtgcccgt tcactgaaca aacggagcat
     1321 atccaaaaac gtgcccgacc gcaacccgcc ctcatggcgg atcgcgctta catgctaaca
     1381 cccgactggt gggactgctg cttcgcagcg tgcgcgtttt caagcagttt gcgtggcttg
     1441 aagttggttc cgtcaaaatc gcgttgtctc gtcccgccca ccagccggta acgcaagccg
     1501 ttgggcaacc ccgtgcaaaa gtaaataatg agagatatgc ttccaataaa tgattacgac
     1561 gcggggacat tcacagctat agcaagcatt tagcaaatta atatgattgg gtttgacatt
     1621 gcgtcggagg ctgtttttca gtaacctgtg atataaaccg agggatgaca caacaaggct
     1681 tcttgatcat ttctgatatt acaggctatt cgaagtatgt caatgagtcg gagctcgaac
     1741 acgcgcgcga cagcctgacc gccttgttga atatattgat cgaccacaca cagtcccccc
     1801 tggtattatc caagctggaa ggcgacgcgg ttttttcata tgcgccagca ggcggatttt
     1861 tgcagggaca aagcctgctg gatatgatcg aatccaccta tgcctccttc cgcaaggcgc
     1921 tagaacttat ggtgatcaac accacctgca cctgcaatgc atgccgaaac ctgcccaatc
     1981 tcgatttgaa gttcttcgtt cattttggtt catatatgac tcagaaattg gggaccttca
     2041 ctgaactggt ggggaacgat gtcaatttgg tgcatcgcct ggcgaagaat cacatcaagg
     2101 agaaaacggg cttcaaggcg tacgctgctt ttacccagtc ggtgatggat gcgttggggc
     2161 ttgtagaatt tcaaaatagc ctgaccccgc atcgtgagac ctttgcggat gtgggggaag
     2221 tgcaaatgta cgtgcacgat atgcataaag tgtgggagcg cttgaaggac agggtgcgga
     2281 tcgaggtccg acctgaagcg gcgatggtcg ttattaaata tgagttcccc gttcctccgt
     2341 ctctcttgtg ggagtacgtg acgaagcctg aatatcgcgt catcctgctc ggagctagca
     2401 aactggagct tcgcaatcag gcacagggac gtacaggagt cgactcggtt ttttactgct
     2461 accatggcaa tatggaaacc cctcaactta ttttggattg gagccccttc gagcagttta
     2521 caacggatga cgccaaccct tgggggttaa ccaccaggat cacatgcaaa ttggagtcca
     2581 cccaggcagg gacgcgtttg attatgttga tgggcaagcc acgcggaaca aggcttcgag
     2641 ttatgctatt cagctcgatc gcaaagttag tttttgtccc acaaatgggc aaggcggcgg
     2701 agacactgca tcagcgcatc ctacaagacc ttgccgatgg aaccgcgttt gtctctccgc
     2761 ccattgaagt tgacaaggct cagatcactt cacttacaac ccaagcgctg acccacggcc
     2821 attagtttca gcaggtcctg ttctaaacca ttgtcaaaat caaagttgct tgtaaaaatt
     2881 gggcgtgtca atagtcgtat ctttttccaa gcggcgtttt ggtaagcaag caggttgccc
     2941 aacacagcgt gcagctgacg gtgggtacgc gccgcgtttt ttggcgtttt tcgtggcttt
     3001 gggtttttcc cgtttcgacg gcaagtcccc ctcccaccca ccgcagctaa cgcacaccgt
     3061 tgggcggcag aaaatagtcc tgcggtaggt ttgtaaaaac agaaaacatg ttctaaactg
     3121 ggcgatattt atccaactcg aaaaggagaa taccatgtcc aatcgaaatg ttgtccacac
     3181 cgagattccc accgctgact ctaacaaagt ggacagttgg ttcgagcgat tagatcaccc
     3241 gcttaaagac gccctaaccg aagtgcgaaa gatcatcctg gctgtggatc cgcggatcaa
     3301 agaaagcatc aagtggggca ctccgacatt ttcatttaac ggtgatattg tcagcataca
     3361 atcaaacgct aaaaagtttg tgagcttgat gtttcatcga ggtgccgaaa tccccggcaa
     3421 gcatgaaggg ttgctgggaa cggctaggct agttcgcacc atgaaattca tcgatctgtc
     3481 ggaggtgaaa cgcagacgca aagaacttga atccgttatc aaagcgtggt gtaggtggaa
     3541 gtcaacgggt gagtaatcag ttattgaatc gatctgggtt gtcgtcccgt atgccgccca
     3601 ccgattaaca gcagtgtcaa gaggtgagca
//
LOCUS       DASTJP010000480         8045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_437432, whole genome shotgun sequence.
ACCESSION   DASTJP010000480 DASTJP010000000
VERSION     DASTJP010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437432"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VFQ23_13880"
     CDS             complement(<1..1514)
                     /locus_tag="VFQ23_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9907737.1"
                     /translation="MNTVDYSFGNWVKRRRKSLDLTQEELAHHVGCSTSLIHKIESDN
                     RRPSRQIAKLLVEYLEIPPDQREIFLKVARQEKAANHLDSLTLPFTAEPVYQSSQQPS
                     LPLALLPIIGREHELHAIGQQLQNPACRLLTLTGPGGVGKTRLALEVGHQQNNSFHHG
                     ACFVSLVGTSTPELIVPAIADGLGFAFSGMTDLKLQLFNYLKEKHILLLLDNLEHLLN
                     GIELLDELLERAPHVKILATSREQLNLRSEWTFDVQGLPIPPSDVKEPESNSAVALFI
                     QRARQMQRDFKPTANDLVSVTRICQFVEGLPLGLELAAGWVKMMPPSEIAREMEQGMD
                     FLTTRARDVPARHRSLRSVFDHSWGLLSLEEQSVLKGLSIFRAGFTRDAAEKVINATT
                     PILSSLVDKSVIRRSDAHVGRYDLHELVRQYAATHLQEAEKTSTSRKHAEYYLTLLEA
                     REPALRSQLQKETLADLRPEIDNLRAAWDFAVASGEIDLLRHATGPLFYFYELH"
     gene            complement(1880..3343)
                     /gene="gtfA"
                     /locus_tag="VFQ23_13885"
     CDS             complement(1880..3343)
                     /gene="gtfA"
                     /locus_tag="VFQ23_13885"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET9907738.1"
                     /translation="MKNKVQLITYADRLGRGDLKTLHQLLKGPLAGLFGGVHLLPFYN
                     PIHGADAGFDPIDHTEVDPGLGGWTDINEIGQDVDLMADLIVNHVSTSSPQFLDYSEK
                     GDSSIYKNMFLTMASVFPKGATEEDLTTIYRPRPGLPFSYTVLKNNEKRLLWTTFSKE
                     QIDINVTDPQGKAYLQSVLQTLHANGIRMVRLDAIGYAIKKPGSSCFMIPETLDFIEY
                     LARQAAALDMEVLVEIHSYFQKQIEIAHQVNYVYDFVLPPLVLHAIFNRTAQHLKQWL
                     EISPRNAITVLDTHDGIGVIDIGADSSDPQTRRGILSPEELDALVEKIHTNSNGQSRL
                     ATGIAASNLDLYQINCTFYDALARNDRDYLLARAIQFFAPGVPQVYYVGLLAGENDMT
                     LLARSGVGRDINRHYYDSEEIYESLQRPVVQSLFELIRFRNCHPAFDGLFSMPETQEP
                     GINLRWDNGVHWAMLEVDFASGSYSISYSPFEALEIK"
     gene            complement(3478..4452)
                     /locus_tag="VFQ23_13890"
     CDS             complement(3478..4452)
                     /locus_tag="VFQ23_13890"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET9907739.1"
                     /translation="MPLTFDLPLEQLGTYQGKNPCPTDFDSFWDMSLSEMRSMDPKVE
                     FVAAEFQAPHVDCYHLFFTGVGGARVHAKLLRPTNASTPHPALLMFHGYTGDSGDWSD
                     KLGYVHAGFTVAALDCRGQAGLSQDGGSVSGNTLHGHIIRGLDDALLGMPEKLLFRQL
                     FLDTAQLASIVMKMPDVDPDRIGVMGGSQGGALAVACAALEPRIKRAAPLFPFLSDYL
                     RVWEMDYAKDAYKELQEYFRHSDPMHKQESITFEKLGYIDIQFLAPRIRANVLWGIGL
                     IDTICPPSTQFAVYNKITSPKQMSVYPDFGHEPLPGFQDQAFRFFMDL"
     gene            complement(4551..5375)
                     /locus_tag="VFQ23_13895"
     CDS             complement(4551..5375)
                     /locus_tag="VFQ23_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9907740.1"
                     /translation="MKAPRGEIWAGRTLLLVLMVITILPFISIFLTALHPSNTVPNGL
                     DWPEDPQWGNFVEAFRVAEMPKTLSSSIFIVLAVVPISLIISTMAGFAIGMLRIPGAN
                     VLLFLFVFGLTLPFPGIIIPIYYLERAMHIYNTRLAIVLPLIALYMPFAVFWMRAHFV
                     NMPAELSEAARIDGATTWDLFWRIHVPLARPPIASLGILMSIWTWNQFLLALVLVEDP
                     TQRTMAGALGYFQGHYATDVPLLCAATILILLPTLGVFLLFQRQVITALLQGSVKG"
     gene            complement(5372..6304)
                     /locus_tag="VFQ23_13900"
     CDS             complement(5372..6304)
                     /locus_tag="VFQ23_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9907741.1"
                     /translation="MTSDDRLVAPTLSRQNYTRKKVRRESVIGWLFVLPALLMYSVFV
                     LLPLLLTVVYSFFRWNGIGPMTWVGLKNYITVFEVPNLLGTITNAFWLVLWFSLIPVG
                     LGLIVASIIHRVATGRFGAIARTVLFLPQVIPLVAAGIIWGWLLSLPGLINQMLKTFG
                     LGAITRAWLGDFDWALPAVGLIGIWVLLGFCIVLLLTGMTKIDPALYESARIDGASWF
                     NEFIMITVPLLRYEIGVCLTVTVIAALAAFDIIWVSTAGGPGNVTSVPGIQIYVLAFT
                     QRSVGLASALAVMLMLLVVVVIIPIQWLSRERTV"
     gene            complement(6358..7791)
                     /locus_tag="VFQ23_13905"
     CDS             complement(6358..7791)
                     /locus_tag="VFQ23_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9907742.1"
                     /translation="MYKKSVIWSLASLVVLVSLLLSACGAPATPAATEAPAITEAATE
                     APVATEAATEAPTEAPVATEAPVSAGPSCGTEPVVLNAYFETGFTIPFDLAEEFTKQF
                     PNVTWDIKQDQFANLITTTPLLLAGDNPPDLLRLPTMVTFAKDGLLMNLDGYAEAFGW
                     DQWPVPQLDQNRVAEDGTRGSGTLYAMGLNYSLTGIFYNKELATQIGMTEPPKTLAEF
                     DALLAAAKEAGLQPIMEWGSAKSGMGLAFPLQNLMAAYGPVGPINDWIFQKPGATIDT
                     PSNLLAAEHLERWIQAGYFPPDINAIEYTDAASRFTQGEGVFTFNGDWQNAGYDTGMP
                     ENVGFFLMPPAEEGGAPAAMSAPLTYGIAANAKNADCAAFFFNWVATNDAARTINVNI
                     GGSNPGGPLGAMMPEAAPGSVTNETLAAGPVVGEAGNSMDFIANATSSIFAQGWTPEL
                     QKMVAGEQDAAGLLEAVQAEYLRELAQ"
BASE COUNT         2043 a         2032 c         2128 g         1842 t
ORIGIN      
        1 tgatgcaatt cgtaaaaata aaacaatggt cccgtggcat gccgcagcag gtcaatctcg
       61 ccgctggcaa ccgcaaaatc ccatgcagcg cgcaagttgt caatttcggg tcgtaggtcg
      121 gcaagagttt ctttttgaag ctgacttctc aaggccggct cgcgtgcctc aagcagggtg
      181 agatagtatt cggcgtgttt cctgctcgtg ctggttttct cagcttcctg taaatgagtc
      241 gccgcatact ggcggaccaa ctcgtgaagg tcataccgcc ccacgtgcgc gtcgcttcgg
      301 cggatcactg atttatcgac caacgaggaa aggatgggtg ttgtcgcgtt gatgaccttt
      361 tccgctgcat cgcgcgtgaa gccagcgcga aagatggaaa gccccttcaa cacactttgc
      421 tcctcgagcg aaagcaaacc ccacgagtga tcgaacaccg agcggagcga gcgatgccgg
      481 gcaggcacat cacgcgcgcg ggtggtgagg aaatccatgc cttgctccat ctcgcgcgcg
      541 atctcgctgg gtggcatcat cttcacccag cccgcggcga gttcgagccc caggggcaag
      601 ccttcgacga attgacagat gcgtgtgacg gagacaagat cgttggcagt aggtttgaaa
      661 tcccgttgca tttggcgggc gcgctgaatg aacagcgcaa ccgcactgtt ggattctggt
      721 tctttcacat cgcttggggg tataggcaat ccctgcacat cgaaggtcca ctcactgcga
      781 aggttgagtt gttcgcgtga ggtggcgagt atcttgacgt gaggtgcgcg ttcgagaagc
      841 tcatccaaca attcgatgcc gttcagcagg tgttcaagat tatccagcag gagcaggata
      901 tgtttttctt tcagatagtt gaataactgc agcttcaaat cggtcatgcc cgagaaggca
      961 aaacccaacc catcggcaat cgccggtacg ataagttcag gcgtgctggt gccaaccagc
     1021 gaaacaaagc acgcgccgtg atggaacgaa ttattttgtt gatgccccac ttccagcgcc
     1081 aggcgcgtct tgcccacacc tccagggccg gtgagtgtga gcaggcggca agctgggttc
     1141 tgaagctgct ggccgatggc gtgcagttcg tgttcgcgcc cgataatggg aagtagggca
     1201 agaggcagac ttggctgttg agaagattgg taaactggtt cggcagtgaa aggaagcgtc
     1261 agcgagtcga ggtggttagc cgccttttcc tgcctcgcta ccttcaggaa tatttcacgc
     1321 tggtctggcg ggatctccag atactcaacg agcaactttg cgatctggcg cgaaggtcgg
     1381 cggttatccg attcgatctt gtgaatcaac gaagtcgaac atcccacgtg gtgagcaagt
     1441 tcctcctgcg tcaaatcaag agacttgcgc ctgcgcttga cccagttccc aaaggaataa
     1501 tccacagtat tcacggtcga accacaatag cccaatcaga atggcggaat tttactacta
     1561 tttatttggt gaattggcag ggagaatcaa aggatgcgat gatacgctcg cgccgataac
     1621 tgcatctggc tcatcgctgc tcacatcaaa ctctgacctt cgtcctgcaa tttgcaaaac
     1681 tgatgtatct ccccaggcgc atatctgcat tactgttgcc aagggccggc acaacacctc
     1741 tatttgtcag ctatggaatc tacatggaca caaatacgcc cacagaatat tgaaaagtcg
     1801 ttaataagta attacagccc tccgtgaatc ataaagtgtg gcactgattt catattacct
     1861 gtagactttc aatatcctgc tatttgatct caagtgcctc aaatgggctg taagaaatgg
     1921 aataggaacc ggaagcaaaa tccacctcca gcatcgccca atgaactccg ttgtcccagc
     1981 gcaggttaat tccaggctcc tgcgtttcag gcatactgaa caatccatca aaagctggat
     2041 ggcagttacg aaagcgaatt aattcgaata gtgattgaac gaccgggcgt tgaagggact
     2101 cataaatttc ttcagagtcg taatagtgtc ggttgatatc ccgcccaacg ccgctcctgg
     2161 ctagcaacgt catgtcattt tcacctgcca gcaagcccac ataatatacc tggggcacgc
     2221 cgggcgcaaa gaattgaatc gcccgtgcca gcaaatagtc acgatcattt cgtgccaagg
     2281 catcgtagaa tgtgcaattt atctgataca aatccagatt ggatgctgct ataccagtcg
     2341 ccaagcggct ttgtccgtta ctgttggtgt ggattttttc aaccagggca tccagttctt
     2401 caggtgagag tatgccccga cgagtttgcg gatcggaact atcagctcca atatcgatta
     2461 caccaatgcc atcgtgggta tccaatactg tgatggcatt gcgcggactg atttccagcc
     2521 actgtttcag gtgctgagcg gtacgattga atatggcgtg caataccagg ggaggcaaca
     2581 cgaaatcata gacgtaattc acctggtggg caatttcaat ctgtttttgg aagtaggaat
     2641 gaatttcgac cagtacttcc atatccaacg ccgccgcctg cctggcgagg tactcaataa
     2701 aatccagggt ttcgggaatc atgaagcaac tgctacctgg ctttttgatg gcatacccta
     2761 tggcatccag acgcaccatg cgaatgccat tggcatgcag agtttgcaac acagattgca
     2821 agtatgcttt gccttgcgga tcggttacat tgatgtcaat ctgttctttc gaaaaagttg
     2881 tccatagtag gcgtttttca ttgtttttca gaaccgtata ggaaaaaggc agccctggtc
     2941 gtgggcgata gatggttgtt aggtcttctt cggtcgcacc ttttggaaag acgcttgcca
     3001 tagtcaggaa catgttttta taaatggatg aatcaccctt ttctgagtaa tccaaaaatt
     3061 gtggggatga agtggataca tgattaacga ttaaatctgc catcaaatcc acgtcctgcc
     3121 cgatctcgtt aatatctgtc cagcctccca gccccggatc aacttcggtg tgatcaatag
     3181 gatcaaagcc ggcatcggcg ccatggatag gattgtagaa cggaaggaga tgaacaccgc
     3241 cgaaaagtcc agccaggggt cctttcaaca attgatggag tgtctttaaa tcgcctcggc
     3301 ccaggcgatc tgcgtatgtg atgagttgta ccttgttttt catgctgggc atctctgggg
     3361 tcgtgttttg tttttgatat ccagggtctg cattctgaga tgtgacgttt ctatctcgca
     3421 aatctctttt ccccaaggca gtttctgggc gaatcaataa agggatgaaa aatcgaacta
     3481 caaatccatg aagaatcgaa atgcctgatc ctgaaaaccg ggtagcggct catgtccaaa
     3541 atcaggatat accgacattt gcttgggcga ggtgatcttg ttataaacag caaactgtgt
     3601 ggaaggcgga cagattgtgt ctattagacc aatgccccaa agcacattgg cacgaatacg
     3661 aggagccaga aattgaatgt caatgtaccc gagcttctca aatgttatgg actcctgttt
     3721 atgcatggga tccgaatggc gaaaatactc ctgcagttct ttgtaagcat cctttgcgta
     3781 atccatttcc caaacccgta ggtaatcact tagaaaagga aataagggcg cagcacgttt
     3841 gatacggggc tccaaagcgg cacaagcgac ggctaacgcg cctccctggc tgccacccat
     3901 gacccctata cgatcagggt ccacatcagg cattttcatc acaatgctgg caagttgggc
     3961 ggtatcgaga aatagttgcc ggaaaagcag cttttcaggc ataccgagta gggcatcatc
     4021 cagtccacgg atgatatggc catgaagggt atttccgcta acacttccac catcttgtga
     4081 taaccccgct tgtccccggc agtccagagc agcaacggta aaacccgcat gaacatagcc
     4141 aagcttatct gaccagtcac ccgaatcgcc tgtatatcca tggaacatca ataaagcagg
     4201 atggggagtt gacgcgttgg tggggcgtaa caatttggca tgcacgcgcg cgcctccaac
     4261 gcctgtaaaa aacaaatgat aacagtcaac atggggtgcc tgaaattctg ctgcaacgaa
     4321 ttcgacctta gggtccattg atcgcatttc agacagactc atatcccaaa aggaatcaaa
     4381 atcggtcgga catggatttt tcccttgata tgttccaagc tgttcaaggg gtaaatcaaa
     4441 ggtaaggggc attttcggct cctgttgtgt cagaaaatgg aaagaaagtc agcaactgtt
     4501 ggtctatcgc gcgtttcacg gttcctgtac tagcctgggc acagcgcggc ttatcccttg
     4561 actgaaccct ggagcaacgc cgtgatcacc tgtcgctgga acaaaagaaa tactccaagc
     4621 gtaggtagca ggatcagaat tgtagcggcg cacaggagag ggacatcagt tgcatagtgg
     4681 ccctgaaaat aacccaacgc gccagccatc gtgcgctgag taggatcttc aacgagtacc
     4741 aacgccagga gaaactggtt ccaggtccag atggacatta atatcccgag cgatgcaatc
     4801 ggaggacgag cgagcgggac gtgaatgcgc caaaacagat cccaggtggt cgcgccatcg
     4861 atacgggccg cttcagagag ctcggcgggc atgttaacga agtgggctcg catccagaag
     4921 acggcaaaag gcatgtataa ggcgatcagc ggcaacacaa tggcgaggcg ggtattgtag
     4981 atgtgcattg ccctctccaa gtagtagatc gggatgatga tcccgggaaa agggagtgtc
     5041 agcccgaaca caaacagaaa gaggagcacg tttgctccag ggatccggag cataccgatg
     5101 gcaaacccag ccatcgtcga gatgatcaga gatatgggta caaccgccaa tacaataaat
     5161 atgcttgagc tgagtgtttt aggcatttcc gccacacgga atgcttctac gaagtttccc
     5221 cactgcgggt cctctggcca gtcaaggccg tttggaacgg tatttgacgg atgcaaagcc
     5281 gtcaggaaaa tactgataaa gggcaggatt gtaatcacca tcagtaccag gagcaaagtc
     5341 cgacctgccc agatttcgcc gcgaggagcc ttcatacggt tctctcccgg ctcagccact
     5401 ggatcgggat gataaccaca acaacaagga gcataagcat gactgccaac gccgatgcca
     5461 ggcccactga ccgttgtgta aaagccagaa catagatctg gatcccaggg acagaagtta
     5521 cattgccagg gccaccagcg gtggagaccc agataatatc aaatgcggcg agcgccgcaa
     5581 ttactgtgac cgtcaggcac acaccgatct cgtaccgcag gagcggaact gtgatcatga
     5641 tgaactcatt gaaccaactg gcgccatcga tccttgcaga ctcatagaga gcggggtcta
     5701 tcttggtcat gcctgtcaac aacaggacga tacaaaaccc cagcagcacc cagatgccaa
     5761 tgagcccaac ggcagggagt gcccaatcga agtcacccag ccaggcgcga gtgatcgcac
     5821 caagtccaaa agttttgagc atctgattga ttaaccccgg cagcgatagt aaccagcccc
     5881 agatgatacc tgcagccacc agtgggataa cctggggcag gaagagcaca gttcgcgcga
     5941 tcgccccaaa ccgacccgtt gccactcggt gaattatgct cgcgacgatg aggccgagtc
     6001 ctactggaat gaggctaaac cacaaaacta accagaacgc gttggtaatc gtccccagca
     6061 agttgggaac ctcgaaaaca gtgatgtagt tttttagtcc aacccaggtc atcggaccga
     6121 taccgttcca gcgaaagaac gagtacacca cggtcaacag caaaggcaaa agcacgaaga
     6181 ccgaatacat gagtagggcc ggcagaacaa acaaccagcc aatgacactc tcccggcgga
     6241 ctttttttct ggtgtagttc tgccgggaga gtgtgggagc cacgagacga tcatctgacg
     6301 tcattgtgtt accactatta acttgtaagg cgcagacatc gtcacgtgga ctcaatatta
     6361 ttgtgctaat tcgcgcaggt attcggcctg aacagcttcg agcaatccag cagcatcctg
     6421 ctcaccggca accatcttct gaagttcggg cgtccagcct tgagcgaaga tcgaactcgt
     6481 cgcattggcg atgaagtcca tggaattgcc agcctcaccg acgacgggtc cagcagccag
     6541 cgtttcattc gtgaccgagc cgggcgcagc ttcaggcatc atcgcgccca ggggaccacc
     6601 agggtttgag ccaccaatgt tgacgttaat cgtgcgtgcc gcgtcgttcg tggcgaccca
     6661 gttgaagaag aacgcagcac agtcggcgtt cttggcattc gcggcgatgc cgtaagtgag
     6721 cggagcagac atcgcggcag gtgctccacc ttcctcagct ggcggcatca ggaagaaccc
     6781 gacattctct ggcataccag tgtcgtagcc ggcgttctgc cagtcgccgt tgaaagtaaa
     6841 gacaccttct ccctgtgtga atcgggaagc cgcatcagtg tactcaatcg cgttgatgtc
     6901 aggagggaag taaccggcct ggatccatcg ttcgagatgc tcagcagcga ggaggttcga
     6961 gggggtatcg attgttgcac ctggcttctg aaagatccag tcattgatcg gtccaactgg
     7021 gccgtaggca gccatcaagt tctgaagcgg gaaggccagc cccataccac ttttggcact
     7081 accccactcc atgatcggtt ggagtccagc ctccttggcg gccgcaagca gtgcatcgaa
     7141 ttcagcgagc gtcttaggcg gctcggtcat gccaatttgg gtcgccaatt ctttgttata
     7201 aaaaattcca gtcaagctgt agttgagccc catggcatat agcgtcccac tgccgcgagt
     7261 gccatcttcc gccacccggt tttggtccag ctgtggaacg ggccactggt cccagccaaa
     7321 tgcttccgcg tagccgtcca ggttcataag gagaccgtcc ttcgcgaacg tgaccatggt
     7381 cggcaggcgg agaaggtcag gtgggttgtc gcccgcaagc agaagcggag ttgtagtaat
     7441 gagattcgcg aactggtcct gtttgatatc ccaggtcacg ttcgggaact gcttggtaaa
     7501 ctcttcagcg agatcgaacg ggattgtaaa tccggtttcg aagtaggcgt tgagcacgac
     7561 gggttctgtc ccgcagctcg ggcctgcact taccggagct tcggttgcga caggggcttc
     7621 agtgggagct tcagtagcgg cttccgtggc gacgggagct tccgtggcgg cctctgtgat
     7681 agcaggggct tctgtggcag caggcgtagc gggagcgccg cacgcggaaa ggagcaggga
     7741 taccaaaact accagacttg ctaaagacca tattacagat ttcttgtaca tttctctttc
     7801 tccttttttt ggtaaagcat ggtcgatatg cgtcttaata tctgattgtt gaattgaatg
     7861 aatattaggg tagacctcct gggatgtaag ttggaaaaga aatacgaagc ttgtgttagc
     7921 aataccttga aagcactttc atatgctagt ctacaacatt tcaagattag agtcaatatt
     7981 tagtttatgc agtttggcgt tctattagcg tcaaaggaag tttgacatgc ctgggtggtc
     8041 tggac
//
LOCUS       DASTJP010000481        15183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1333918, whole genome shotgun sequence.
ACCESSION   DASTJP010000481 DASTJP010000000
VERSION     DASTJP010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333918"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..525)
                     /locus_tag="VFQ23_13910"
     CDS             complement(196..525)
                     /locus_tag="VFQ23_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HET9907743.1"
                     /translation="MDEDNFPIEFNNEIDDVKKSENEYYTLAVDRLSKLAEGHNDISG
                     GVVNLKQPAQGHQTPYIYEVTIVVYRGSDHIAATEKGEQFESTLDGALDAVERQVRES
                     RKQQRNY"
     gene            complement(616..1773)
                     /locus_tag="VFQ23_13915"
     CDS             complement(616..1773)
                     /locus_tag="VFQ23_13915"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907744.1"
                     /translation="MENLTLDQLKNLAQQTASPSISIFLPTHRAGQDTQQDPIRFKNL
                     LRDAEKQFLSSGMGPREVSALLQLAQALLDDSYFWMHQYEGLAVFMTSEDFHSYRLPF
                     KVEELLIIAQSYYIQPVLPLFTNNGHYYILAISQDEVRLFEGTRHGVGQIDLPDGTPE
                     NLNEALRLDDPQKQLQMHTGSSQSATGHGMFHGQGAGDEEQKVRIERYLNLVDTGLKE
                     IFREQKAPLVLAGVDYLLPIYRKVSEYANIMQEGITGSPEHLRPEELQEQAWPIVEAY
                     FRQETVIAVEQYEQHAAMDKATDDVKEIVAAAFNGRVDKLILSVETQVWGTFNPTTRE
                     VILDSDGQGKQNSLALLDFAVMKTLQNGGTVYALSQEEMPTESPIAVVFRY"
     gene            complement(1828..2001)
                     /locus_tag="VFQ23_13920"
     CDS             complement(1828..2001)
                     /locus_tag="VFQ23_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907745.1"
                     /translation="MNQLLRSYMTQTAAHQVITLTAGDLFPGYNGCVAILHVPDQELE
                     VVACWRAETRSIQ"
     gene            complement(2042..2389)
                     /locus_tag="VFQ23_13925"
     CDS             complement(2042..2389)
                     /locus_tag="VFQ23_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9907746.1"
                     /translation="MSTERKSQPIIDELGLPKNGIFDRERKRIELEESEVRYRRLFET
                     AKDGILILDGDTGRITDVNPFLQDLSGYSKTQLIGMALWEIGAVKDIAASQEAMRHLQ
                     HSEYRQRTNGFGR"
     gene            complement(2539..3399)
                     /locus_tag="VFQ23_13930"
     CDS             complement(2539..3399)
                     /locus_tag="VFQ23_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9907747.1"
                     /translation="MTSYERRLSLRDYQYRRPEVRLPETPEAPENIAGQTHAPFDDLQ
                     RSNVQLREHINALSTRNKELEDYAHMVAHDLKEPLTVLILTADLIKDVPDLTGEELSE
                     WLLQIRSTAYEMKSIVKNLLFFAEVSKVEAPRGSVHMSQVVANVKSRLSHMIQEKQAQ
                     LILPEVWPDAFGYGPWIEEVWANFLSNALKYGGRPPRVELGASTSPGGMLRFWMRDNG
                     PGIPPEARTRLFIRSNQINRINTLGHGLGLSIVGTIIKKLGGQVGVESEVGKGSLFFF
                     TLPAGISSTE"
     gene            complement(3657..4682)
                     /locus_tag="VFQ23_13935"
     CDS             complement(3657..4682)
                     /locus_tag="VFQ23_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET9907748.1"
                     /translation="MNFAMWNKALKVIPQVSRAEWNHLDFVSKWLISTRAAVLVMTLI
                     SAMLAGLFAIRDNLFQPLPWLALAFGLIMAHASNNLFNDYTDYVRGVDQKNYYRAMYG
                     PQPLVDGLMTKGQNLIYFVISACLALASGLYLTWYNNFDTLLWILIGLGAFFILFYTW
                     PLKYLALGEVAVVIVWGPLMIGGGYYVLTHNWDWNVVWATLPYALGVTTVIFGKHIDK
                     IEADQRKNIRTLPVLFGERVARQTVIGMMILPYFLLVYLVATKYFTPLLLIVLLSIPR
                     LRMILPAFLTPKPATRPQDFPEGQGGWPLYFAPLAFVYNRSFGTLFVLGLIADVLIRR
                     LLPAYWR"
     gene            complement(5000..5755)
                     /locus_tag="VFQ23_13940"
     CDS             complement(5000..5755)
                     /locus_tag="VFQ23_13940"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9907749.1"
                     /translation="MNNKLFEDKVGLVTGGASGIGEACALTFARGGAKVAVSDLNTEL
                     GEKVVAEIKQAGGDAIFLKVDVSDSQAVEKMVTDTVTAFGKLNIAVNNAGIGGEQNST
                     GNYSIDGWNKVMNVNLNSVFYCMRYEIPEMLKAGSGAIVNMSSILGSVGFAGSPAYVA
                     AKHGVLGLTKSAALEYGKQGIRINSVGPAFIKTPLLNTLDESTQAYLSGLHAVGRMGT
                     SQEVADLVAFLCSDQASFMTGGYYLVDGGYTAQ"
     gene            complement(5945..6217)
                     /locus_tag="VFQ23_13945"
     CDS             complement(5945..6217)
                     /locus_tag="VFQ23_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HET9907750.1"
                     /translation="MSAKKHFTMEEAREIGGKLGIDWSRFDVEQFRMGLDVELEHGLH
                     DPSTNVTGNDPIITGKIALAHLNEFADYYTRLEKMEQAAEEKPVQN"
     gene            complement(6321..7541)
                     /gene="nirK"
                     /locus_tag="VFQ23_13950"
     CDS             complement(6321..7541)
                     /gene="nirK"
                     /locus_tag="VFQ23_13950"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HET9907751.1"
                     /translation="MEVISYLWQYIVEVFGLFIAYVFAAIFIFLVLRQFHSRKITAAL
                     VAIFIGISIAFYVPHGMPEELAYPLSLNSFGPGERPDLPIRNAFRFFQNFKNFERISD
                     IAKHPADLPKAVVYGDDGVVEINLTTREVIAEMADGTTFNYWIFDNTVPGPFLRVRVG
                     DTVRMTIHNDETSLHPHNIDLHAVTGPGGGAAATIVAPGESKTLTFKALNAGLFIYHC
                     AFGNPGLHMTHGMYGLILVEPEGGLPAVDKEFYIVQGEFYSSSTLGRKGLQLFDTQDY
                     LDGKPQYIVFNGRTGALTEGMTANVGDIVRMYVGNGGVNLVSSFHVIGEIFDRVYREG
                     DLVSLPAEGLQTTLIPAGGAVMVEFKVDYPGNYVLVDHALARVDRGAWGVLHVEGEAD
                     SSIFDGTIQSGDGH"
     gene            complement(8131..8385)
                     /locus_tag="VFQ23_13955"
                     /pseudo
     CDS             complement(8131..8385)
                     /locus_tag="VFQ23_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
     gene            complement(8567..9214)
                     /locus_tag="VFQ23_13960"
     CDS             complement(8567..9214)
                     /locus_tag="VFQ23_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9907752.1"
                     /translation="MTTSPSKPIRVMLVDDHTMVRRGLAAFLKVFDDLQLAGEAESGA
                     EAIQLCATILPDVILMDMVMPDMDGATATRAIRQGFPQVQVIALTSFKEGDLVKNALK
                     AGAIGYLLKDVSADELVWAIRAAHAGRATLSPEAAQALVETANQPAAPGLDLTERERE
                     VLTLMIEGLNNTQIAGKLTVSPSTIKSHVSNILAKLGVASRTEAVTLALRNRIVS"
     gene            complement(9211..10776)
                     /locus_tag="VFQ23_13965"
     CDS             complement(9211..10776)
                     /locus_tag="VFQ23_13965"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9907753.1"
                     /translation="MKQVPDRRLTSEEEYRNIFEAASDGLIIYDIGTDLVVEANPAAC
                     EMHGYTRQEFIGLNPVVFLPPESHDLFREFVRTAEPGSVFDSLIIHLRRDGSPFHAEV
                     RRSVINYRGRPCLLSAIRDVSQRIQTEKNLSEEIEARVREQATLLAISHTLASTLEFQ
                     PGLILDQLREIIEYSHGGIFLLQDSTLTTMAMRGTPRLEQSSPFHIPLQAPETLAALF
                     NVNKPIRIAEVWNDNPQARLLRALLDDEAAVLLDGMHSWMWVPLAVKGRIIGGVGVAH
                     ETKNYFTPHHADLALSVANQAAITMVNAELYKDAQTLAVLEERQNLARNLHDAINQSL
                     FSAGLIAEVLPRLWEREPEEARRALEDLRRLTRGALAEMRSLLVELRPSALTDTDLGD
                     LLRLLGNAFTGRTNIPVSVKVKGDGSLPAKVQVVFYRICQEALNNIAKHAKASQVEID
                     VQHEPDSLELSIRDDGRGFDTAPQIPSGHYGLSMMRERAETVGALLTLTSQPGHGTEL
                     MIRWSQAGRKEVV"
     gene            11052..11495
                     /locus_tag="VFQ23_13970"
     CDS             11052..11495
                     /locus_tag="VFQ23_13970"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9907754.1"
                     /translation="MNKREIVQTLLDSVQKGDFETAKSMLADDFQFSGPIPEPINRDA
                     WLAMSSSLRTAFPDLDYHFKVIGTDGEFVRSTSQLSGTHRGAFDLTNMNMGVIPATHQ
                     SFASKVEKTKITVKEDKITSWAVEPTDGAGLKAILDQLGVKVPTM"
     gene            11637..11912
                     /locus_tag="VFQ23_13975"
     CDS             11637..11912
                     /locus_tag="VFQ23_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET9907755.1"
                     /translation="METKIYVGNLAKSTSLDDLNSLFAQAGQVMLVDMIKDRKSGEAK
                     GYAFVTMSAQSEADKAISMFNAYSLREQELKVNLAKPREQRGFDTKN"
     gene            complement(12790..12861)
                     /locus_tag="VFQ23_13980"
     tRNA            complement(12790..12861)
                     /locus_tag="VFQ23_13980"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12826..12828),aa:Arg,seq:ccg)
     gene            complement(12884..13870)
                     /locus_tag="VFQ23_13985"
     CDS             complement(12884..13870)
                     /locus_tag="VFQ23_13985"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET9907756.1"
                     /translation="MALLGAFFFGFVPMFLFAAFVNWLDRYEKEPKLLLGAAFLWGVV
                     IAGGGAFVLNTLFGISIYALTGSEGAAEFGTTSIVAPIIEEALKGLAVLVVFVLFRKE
                     FDSILDGIVYGAITAMGFAAIENVLYIYRNGFLESGWEGFWVLVFIRVVLVGWMHPFF
                     TAFTGIGLAIGRMSKNILVKLVAFPAGYSVAVLTHAFHNTFSSVVGGGGGFILGLLAD
                     YFGYALMSVFIIWMIIHERNILKKNLREEVASGSISQQQYNTAISFFQTNAHLSALSS
                     GSFGPTRRFYQVLGELAHKKEQLAQVGDEGGNTQLINKLRSELVQLAPLAKA"
     gene            complement(13897..14529)
                     /locus_tag="VFQ23_13990"
     CDS             complement(13897..14529)
                     /locus_tag="VFQ23_13990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HET9907757.1"
                     /translation="MRIRITFAKQGALRYTGQLDLHKLWERAARRAELPLAYSQGFHP
                     QPKINIAAALPLGFSSRCEVMDMKLEQEIPLEGLREKLQGTLPTGIQVLNVESTEERA
                     PSLQTQVIAAEYEVTLTEPVEGSDLKRKVDSLMESESIIRERRGKKYDLRPLIESLTT
                     LPEGEEQGTTVFMRLAAREGATGRPEEVLDVLGIAFEGTRIERTRLIFQS"
     gene            complement(14529..15062)
                     /locus_tag="VFQ23_13995"
     CDS             complement(14529..15062)
                     /locus_tag="VFQ23_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907758.1"
                     /translation="MTTKKQFISKLLQERDKFELLLNRVGYSRRMTLKGVSGKWSIKD
                     VLAHILAYEQYIADRMNEILHGEQYTPCRTQTALDAFLDEFGYPDFGSSLLDDDGPNA
                     WIVEKYQRVSLEDVVSQELQAFSSVISTLEKLSDDQIQQHNLFERVANNTYKHYQEHI
                     RDIRNWLKTNAINSNKS"
BASE COUNT         3793 a         3857 c         3645 g         3888 t
ORIGIN      
        1 tgggtgattt agtttgcaat aatcatccaa gcgcgtcctt cagacttggc gcgataaacc
       61 gcatcgccac atatttagcg atgaactccc acggtcgatt attattcatg gctgtactcc
      121 ttctgtgaat catcgtaacc aggcctcgtc tacttcagtg tgattgaccg cgttaacgcg
      181 gtcaatcacg ataatttaat agttacgctg ttgtttacgc gattcacgaa cttgccgttc
      241 aaccgcatct agggcaccat ccagcgtgga ctcgaactgc tcaccttttt cggtagctgc
      301 aatatgatct gatcccctgt agactacaat cgtcacttcg tatatatacg gtgtctgatg
      361 cccttgggca ggctgcttca aattaacaac tccgccggaa atgtcgttgt gaccttctgc
      421 taatttgctg agccggtcta cagccagggt atagtattcg ttctcacttt tcttcacatc
      481 atcaatttca ttattaaact caataggaaa attatcttcg tccatttttc ttcctttcct
      541 tcggcctttt gaataacgat ttcttatcct gatagccgtg tgattttttc tcgagtttca
      601 accagattgc tagccctaat agcggaaaac aactgcgatt ggcgattccg taggcatttc
      661 ttcctgagaa agtgcgtaaa ccgttccacc attttgaagg gtcttcatga ccgcaaagtc
      721 caatagcgcg aggctgttct gtttaccctg tccatccgaa tcaagaataa cttccctggt
      781 cgtgggattg aatgtacccc acacctgtgt ctctacggat aagatgagtt tgtccacacg
      841 accattaaac gcggccgcga caatctcctt aacgtcatct gttgccttat ccatagcggc
      901 atgctgctca tactgttcca cagctattac cgtctcctga cggaaatagg cttccacaat
      961 cggccaggct tgttcctgta attcttcagg ccgcaaatgc tcagggcttc cggtgatacc
     1021 ttcctgcatg atattggcat attcgctgac cttgcgataa atcggcagca gataatcaac
     1081 cccagccaaa accagcggtg ccttctgttc acgaaagatt tccttgagtc cggtgtccac
     1141 taggtttaga taccgctcaa tccggacctt ctgttcctca tcgccggcac cctgtccatg
     1201 gaacatgcca tgacctgttg cgctttgcga agatcctgtg tgcatctgca actgcttttg
     1261 tgggtcatcg agcctgaggg cttcgttcag attctcaggt gtcccatctg gcagatcaat
     1321 ttgacccacg ccgtgacgtg ttccttcaaa caatcgaacc tcatcctggc tgattgccag
     1381 gatgtaataa tggccattgt tagtgaagag cggtagaaca ggttggatgt aataggattg
     1441 agcgatgatg agcagttctt cgaccttaaa tggaagacga taggaatgga aatcttccga
     1501 cgtcataaat acagccaatc cctcgtattg atgcatccag aaataggaat catccaataa
     1561 tgcctgtgcc agttggagta acgcacttac ttcgcgaggt cccatcccgc tactcagaaa
     1621 ctgcttctca gcatcacgaa gaaggttttt gaaccgtatc ggatcctgct gggtatcctg
     1681 accggcacga tgtgtcggca gaaaaatcga tatacttggg ctcgccgttt gttgagccag
     1741 attcttgagt tgatctaaag tcaagttttc cattactaac tccttcaatt tttcatgcat
     1801 agcaaactat gctgcggatc gaccacttca ctgaattgac cgcgtctcag ccctccaaca
     1861 cgccaccact tcaagctcct gatccgggac atgcaaaata gcgacacagc cgttgtatcc
     1921 gggaaataaa tctcctgcag tcagagtaat tacctggtgc gctgcagtct gtgtcatata
     1981 ggatcgcagc agctgattca tgtgattgaa taactgcatt gatctgtccc gccattgagg
     2041 tttatctacc aaagccattc gttcgctgtc tgtactcgct gtgttgcaag tgccgcatgg
     2101 cttcctggct ggctgcgatg tccttgacgg cgccaatctc ccagagtgcc atgccgatca
     2161 actgggtctt agagtaacct gacaaatcct gaaggaatgg gttaacgtcg gtgatccgtc
     2221 ctgtgtcccc atccagaatc aggattccgt ctttggctgt ctcgaacagc cggcggtagc
     2281 gaacctctga ttcctccaat tcgattcgtt ttcgctcacg gtcgaaaata ccattcttcg
     2341 gcaacccgag ttcgtcgata atgggttgcg attttctttc agttgacatt gtagtggcct
     2401 atctattttc gaggtgccag ccaagtaggc cctgccagta aattcatttt atagcaacgc
     2461 gccctggttc gcatcgacca gtcggggaat tcctatctcc gccacttggg ggagattcgg
     2521 agcatcagag taaaggcctt actcagtgga agatatgccg gctggcaagg taaagaaaaa
     2581 cagactgccc ttgcctactt cgctttcaac gccgacttga ccacccagtt tcttgatgat
     2641 ggtgcccacg atggacaagc ccaatccatg ccccagagta ttgatgcggt tgatctggtt
     2701 ggaccttatg aatagacgcg tgcgtgcctc aggtgggatg cccgggccgt tgtcgcgcat
     2761 ccagaaacgg agcataccgc ctgggctggt tgaagcgccg agttcgacgc gcggcggacg
     2821 ccctccatac ttgagggcat tactgagaaa gttagcccac acctcttcga tccatggccc
     2881 gtagccgaaa gcatctggcc agacctccgg gagaatgagt tgagcctgct tttcctggat
     2941 catgtggctc agccgggact tgacgtttgc cacaacctga gacatatgca ccgatccgcg
     3001 cggtgcttcc accttgctca cttcggcgaa gaacaataag tttttaacaa tacttttcat
     3061 ttcgtatgcg gtggacctga tctgaagtag ccattcgctc aattcctccc cggttagatc
     3121 ggggacatct ttgatcaggt ctgctgtcag aatcaggacg gtcaatggtt ccttaaggtc
     3181 atgggcaacc atgtgcgcgt aatcttccag ttccttgttg cgcgtgctca gggcatttat
     3241 atgctcgcgc agttgcacat tcgagcgttg aaggtcatcg aagggcgcat gtgtctggcc
     3301 cgcaatattc tctggggctt caggtgtctc tggcaacctt acctcgggcc ttcggtattg
     3361 ataatctcga agcgagaggc gtcgttcgta ggatgtcatt gtcacggtgg caaccctttc
     3421 tagatgtcag attaaaattg caaacttcag taaatttata ataaaacaaa acttcccctg
     3481 ccacatccat cagtcgggga atttttatct cccccagttg ggggatgctc cgtttatagt
     3541 tctttttcaa ctattgcaat actcaaagat gaccgcgcat tatcgtaccg ataaaccatc
     3601 ccatatgcat cccgcacctg agatggttgt tctgagccac ttggaggaga aggcgattaa
     3661 cgccagtatg ctggcagcaa tcgtcgaatg agcacatccg caatcaggcc aaggacaaag
     3721 agcgtaccga acgaacggtt gtacacgaac gccagcggtg cgaagtaaag gggccagccg
     3781 ccctgaccct ccgggaagtc ctggggacgt gttgccggtt ttggcgtgag gaaggctggt
     3841 aatatcatgc gtagacgcgg gatagatagc agaacgatca atagtagcgg cgtgaaatat
     3901 ttggtcgcaa ccaaataaac caacagaaaa taaggcagga tcatcattcc aatgaccgtc
     3961 tggcgggcga ccctctcacc aaacaggaca ggcagagtac ggatattttt tcgctggtca
     4021 gcctctatct tgtcaatatg cttgccaaag atgactgtgg ttacccctaa agcatagggc
     4081 agggtggccc aaaccacgtt ccagtcccag ttatgggtaa gcacataata tccaccgccg
     4141 atcatgagcg gtccccacac gatcacaacc gccacttcac ccagagccag gtacttcagc
     4201 ggccaggtat agaagaggat gaagaacgca cccagcccaa tcagaatcca gagcaatgta
     4261 tcgaagttgt tataccaggt caggtatagg ccactcgcga gagctaggca tgcactgatt
     4321 acaaagtaga tgaggttctg cccttttgtc atcagaccat ccacaagagg ttgaggacca
     4381 tacatggcgc gataataatt tttctgatcc acaccacgca cataatcggt atagtcattg
     4441 aacagattat tactggcgtg tgccatgatc agaccaaagg ctaaggccag ccacggaaga
     4501 ggttgaaaaa gattgtcgcg tattgcaaag agacctgcca gcatcgcgga gatcagggtc
     4561 atcactagca cagcggcgcg ggttgagatc agccattttg agacaaaatc cagatgattc
     4621 cattctgctc gtgaaacttg cgggatcact ttcagcgctt tattccacat tgcgaagttc
     4681 atactgaaat ctcctttcgt atccattctc ttttaggaaa gctgtttctc agggttcata
     4741 tgccgaattt gagagattaa ttacgcaaaa ccgagggcgt gatgaaaggg caaggttggt
     4801 aaattatcta atccaaaaat gcctcaacat aagggcagcc agattatcca tgagattaag
     4861 tatgcaattc cgaaatatgt aagtaattgt ttcatcctct tccttatcct ggcgttcttc
     4921 ggcttcctgt tctatccata tcaaagcgtt cttttggcat cgggtggtca cagcaaaagg
     4981 atgatcgcaa ttactattgc tattgagcag tatatccacc atccacaaga taatatccac
     5041 cagtcataaa cgatgcctgg tcagagcaga gaaaggcaac caagtcagcc acttcctgcg
     5101 acgtacccat acgtccaacg gcgtgcaatc cactgaggta ggcctgcgta gattcatcca
     5161 atgtattcag cagtggagtt ttgataaaag caggacctac agagttgatt cgaattccct
     5221 gtttgccata ttcaagtgct gcgcttttgg tcagccccaa aactccgtgc tttgctgcca
     5281 catatgctgg agagccggca aacccaactg agcccagaat ggatgacatg ttaacgattg
     5341 caccagagcc cgccttcagc atctctggga tttcatagcg catgcaatag aaaacgctgt
     5401 tgagatttac gttcattact ttgttccagc catcaatgct atagttgcct gtagaattct
     5461 gctcaccgcc tatcccagca ttattgactg cgatatttaa tttaccaaag gctgtaactg
     5521 tatctgtaac cattttctcc acagcctgtg agtctgatac gtccactttg aggaagatgg
     5581 catcgccacc agcctgcttt atttcagcga cgactttttc gccaagctca gtatttaaat
     5641 cactaactgc cacctttgcg cctccacgtg caaaagtaag tgcacaggct tcgccgatac
     5701 cagaagcacc accggtaact aatccaactt tatcttcaaa cagtttattg ttcataggtt
     5761 ttctcctggt caggctgaac cacctgacga taagataata acgaattttt tgcttgtagt
     5821 ttatgctagg taagcaacag agcaatcatc ccctagatca gtatttacct taggcatgtg
     5881 gatagtatta caaagaactg aatgacgtgt ttatttcaga tactgataat cttctttgat
     5941 agtgttagtt ctggacaggc ttttcttcgg ctgcctgttc catcttttcg agacgagtat
     6001 agtaatcagc gaactcgttc agatgagcaa gcgcgatttt gccagtgatg atagggtcat
     6061 tacctgttac gtttgtcgat ggatcgtgca agccatgctc caactcaaca tccaaaccca
     6121 tgcggaactg ctccacatcg aagcggctcc aatcgatacc taatttgccg ccgatttcgc
     6181 gagcttcctc cattgtgaag tgctttttag ctgacatttt cttctccttt ttgatatttc
     6241 tttaggttcc tgatgtatcc attcatggtc tgatgagtga gcattttgtc gaaagcttcc
     6301 tggcacgggt tgacaataat ctaatgacca tctccgcttt gtatcgtgcc gtcaaaaata
     6361 gatgaatctg cttctccttc tacatgcagc actccccagg caccgcggtc aactcttgcc
     6421 agcgcatgat ccacaagaac ataatttccg ggatagtcaa ccttgaattc caccatgacc
     6481 gcgccgcctg ctggaataag cgtggtttgc aaaccctctg ccgggagact caccaaatcg
     6541 ccttcgcgat agacacggtc aaatatttcg ccgatgacat ggaaacttga aactagattg
     6601 actccgccgt tgccgacata cattcgaact atatctccaa cgttcgcagt catgccctca
     6661 gtaagtgctc cagtccttcc attgaagacg atgtattgcg gcttcccatc cagataatct
     6721 tgtgtgtcaa aaagttgaag gccctttctg cctaatgtac tggaactata gaactctcct
     6781 tgcacgatgt aaaattcctt gtcaacggca ggcagtcctc cttctggctc aaccagaata
     6841 agcccgtaca taccgtgagt catgtgcaga cccggattcc caaatgcaca gtggtagata
     6901 aataacccgg cgttcagtgc cttgaatgta agtgtcttgc tctccccagg tgcgacgatg
     6961 gtcgcagctg cgcctccgcc ggggcccgta accgcatgga gatcaatatt atgaggatgc
     7021 agactggtct catcattgtg gatcgtcata cgaaccgtgt ctcccactcg tacgcgtaga
     7081 aacggaccgg gaacagtgtt atcaaagatc cagtagttga aggtggtgcc gtcagccatc
     7141 tcagcgataa cttccctcgt ggtcaaattg atctcaacga caccatcgtc accataaaca
     7201 acagcttttg gcaggtctgc tggatgcttg gcgatatcag atattcgctc aaaattcttg
     7261 aagttctgga agaagcgaaa cgcgtttctg atgggcaggt ctggtctttc gcccggaccg
     7321 aaagagttca gggaaagagg ataggccaat tcttctggca tgccatgagg tacataaaac
     7381 gcgatggaaa tcccgataaa aattgcaacc agggcagcag ttatcttgcg ggaatgaaat
     7441 tggcgaagca ccagaaagat gaaaatcgct gcaaatacat aggcaatgaa gagcccgaac
     7501 acttcaacga tgtattgcca aaggtatgag attacttcca tctgttctcc ttgttgggct
     7561 gaattgcctg acgaacgaat aacagggatt ttgtcggtat gggtggagac acccgacatg
     7621 tagataatac aattcctttg tctgccttgc caaattcagg ctgaaatcag gattggcagt
     7681 gttgattggc tgctgggcgc gctaacggat ggatttacca taaagatcgt ccacgccata
     7741 ttccatcaca acataacgca catggttaca gctggtcacc acgcctgcac cttgcttcag
     7801 ttagttaccc ttctttcatc ctacaacaaa cactccccaa ccacatcaac cggtcggggg
     7861 gtgtttgtct ccgtcacttg ggggagacag gaggctaggt tttctttgcc gcgtatatat
     7921 gtagcagcaa gagcgcgtca tcctcagtgg gcagaatcaa ccaagcataa atttgcccga
     7981 gcggtgacgt acaaaaccat catggagtga aatttaagtt gcggcagaaa tgttaggcaa
     8041 acgccctagt ttaacttcca gcttgacggg agctctccct ttagattcac ttgacaactc
     8101 agttgtatca gaagtactgg agattggccc cggctcgaca accagcttga tcgctgttct
     8161 cacttgatct tcaatggtca cggcggcgaa ttcaggcacg catctgacat agatttcatc
     8221 cagccgaaga aaactggttg ctaaaatgag cgctttaatc gcctgattga ccgcggctgc
     8281 accgatggct tgaacttctg cccgctgatg ttcgcgaact acgccggcaa ttgcgccagc
     8341 aacagcagct gtgcgcgaag tggcagatac tttgatcata ttcatgggag gtctcctcaa
     8401 cagtgaaaaa tggttttggt atggtgcttg gcttttgatc tcagaaggtg gaaagactgg
     8461 cagagtaaat cacaaaatat tttcctaatg ttataacaaa cccttcccag ccacatccat
     8521 caactgggga ggatttgtct ccgccacttg ggggaggtgt ggcggttcag gaaacgatgc
     8581 gattccgcaa tgccagtgtt acggcctcgg tgcgactagc aacgcctaat ttggccagga
     8641 tgttactgac atgcgatttg atcgtggatg ggcttaccgt cagttttccg gcgatctgcg
     8701 tgttgttcaa tccctcgatc attagggtta ggacttcccg ctcacgttcc gtcaggtcga
     8761 gtccgggcgc tgctggttga ttggctgtct cgaccagggc ttgcgccgct tctggggaaa
     8821 gtgtggcccg accagcatgt gcagcacgga tggcccacac tagttcatct gccgagacat
     8881 ccttgagtaa atagccaatc gcaccggctt ttagagcatt cttcaccagg tccccctcct
     8941 taaaagaggt caatgcaatc acctgcactt gcggaaatcc ctggcggatg gcgcgggttg
     9001 cagttgcccc gtccatatct ggcatgacca tatccatgag gatcacgtcc ggcagaatcg
     9061 tggcgcagag ttggatggcc tctgcaccac tctcggcttc gcccgccagt tgcaggtcat
     9121 caaagacctt aaggaaggcc gctaggcccc ggcgcaccat ggtatggtca tccaccagca
     9181 tgacgcgaat tggtttggaa ggggaggtgg tcatacaact tcctttcttc cagcctggct
     9241 ccagcggatc atgagttcgg tgccatggcc aggctggctc gtcaatgtca acaacgcgcc
     9301 gacagtttcg gcacgctcac gcatcatact caagccataa tgacccgatg gtatttgcgg
     9361 cgcggtgtca aaaccgcgcc cgtcatcccg aatggatagt tccaatgagt ccggctcgtg
     9421 ttgcacatcg atctcgacct gactggcttt tgcatgtttg gcaatgttgt tcagggcttc
     9481 ctgacaaata cggtaaaaca ccacctggac tttagccggc agtgagccgt cacctttcac
     9541 tttcacagaa accgggatgt tcgttcttcc tgtgaaagca ttacctagca atcgcagcag
     9601 atctcccagg tcagtgtccg tcagggcgga gggtcgcaat tcgaccagca aggagcgcat
     9661 ttcggccagc gcaccgcgtg tcaaccgacg taaatcctcc agtgcacgcc gggcttcctc
     9721 cggttcacgt tcccataaac gcggcaggac ttcggcgatc agaccagctg agaagagcga
     9781 ctggttgatg gcatcgtgca ggttgcgcgc caaattctgg cgttcctcca ggacggctaa
     9841 cgtttgcgca tctttataaa gttccgcgtt gaccatggtg atggcggcct ggttggcgac
     9901 gctgagagcc aggtccgcgt gatggggcgt gaagtaattc ttcgtctcgt gtgccacacc
     9961 tactccgccg ataatgcggc ctttgaccgc cagcggtacc cacatccagg agtgcattcc
    10021 atccagcaaa acagcagctt catcgtccag aagcgcgcgc agaagtcgtg cctgcgggtt
    10081 gtcattccat acctcggcga tgcgaatggg tttgtttacg ttgaatagcg cagccagagt
    10141 ttcaggagcc tgcagcggga tgtgaaaagg tgacgattgt tctaatcgcg gcgttccgcg
    10201 cattgccata gtggtcagcg tggagtcttg caataagaat attccgccat gactgtactc
    10261 aatgatctct cgcagttggt cgaggatcaa acccggctgg aattcaagcg tggaggccag
    10321 ggtatgcgag atggcgagca gcgtggcctg ttcgcggacc cgcgcttcta tctcttcgct
    10381 taggtttttt tcggtttgaa tgcgctggct aacatctcga atggcgctca acaggcacgg
    10441 ccggccccgg tagttgatca ccgatctgcg aacttcagcg tgaaacggtg agccatctct
    10501 ccgtagatgg atgataagag agtcgaatac actgcccggt tcagccgtcc ggacaaactc
    10561 ccgaaacaag tcatggcttt caggaggaag gaagaccacg gggttcagac cgatgaattc
    10621 ctggcgggtg tagccatgca tctcgcaggc cgcggggttg gcttcaacta ctaggtccgt
    10681 cccgatatca tagataatca acccatcgct tgcggcttca aagatgttcc ggtactcttc
    10741 ttcagaagtt aaccggcgat caggcacttg cttcatcagg cttacctctt gctttctgat
    10801 gtccttcagt tatccattca ttgtactgca atagataagt gtcatttgga atgaaaacgt
    10861 gaaagtcgca aagaatgtcc agtttcatag ttccgcgtat tatgacccat tactgtgtta
    10921 gactaataca ggcgtatcgt aaacaagttg tttatcatgt tgggggaaga ggagggtgtt
    10981 ccatagggaa catccattat tatccaggcc catttgctct aaaactgctc gacgaaaagt
    11041 aaaggattag aatgaataag agagaaatcg tacaaaccct attggattcc gttcagaagg
    11101 gtgacttcga gactgccaag tctatgctgg cggatgattt tcaatttagc ggacccatcc
    11161 ccgaacctat caacagggac gcctggcttg caatgagttc cagcttgcgg accgccttcc
    11221 ccgacctgga ctatcatttc aaggtcatcg gcacagatgg cgaattcgta aggagcacct
    11281 cacaactgag tggaactcac cgtggcgctt ttgacctgac gaacatgaac atgggtgtga
    11341 tccctgctac gcatcaatcc tttgcatcga aggtcgaaaa gacaaagatc accgtcaaag
    11401 aggataagat cacttcatgg gctgttgagc cgacggatgg ggcgggactg aaagcgattc
    11461 tggatcagct gggtgtcaaa gtaccaacga tgtagtcctg cacgagtttg aggttgaaac
    11521 ctggatatac agttttgaac tgtatgactg gtgtaatcta gtatctcagc cgctcttcaa
    11581 aaatgagccg tagctgtgtt tttgagccgg tgatcccgga gaaagtagtt ttcacgatgg
    11641 aaaccaagat atatgtagga aatctggcga agtccacgag cctggatgac ctgaatagtt
    11701 tgtttgcaca agccggacag gttatgctgg tggatatgat caaggaccgt aaaagcggtg
    11761 aagcgaaggg atatgccttc gtcacgatga gcgcgcaaag cgaggcagac aaggccatca
    11821 gtatgttcaa tgcatattcc ctcagagagc aggaattgaa ggtaaacctg gctaaaccga
    11881 gagagcaacg tgggtttgac acaaaaaatt aagttttgtg aaccaacctt ccccgcctca
    11941 cggcggggtt tcttttcaaa ggaagaataa tgatagatgt taacatgaat acccggatca
    12001 atcgatcccc gaaggaggtc tttagctttc ttgtggcaca tgagaatgat tctcaatggc
    12061 agtatggaac atttgcgtca gcccggattc ctcgacgcat caacgataca aaaaactttt
    12121 ttcgaagtat aggtcatttg atgggccgta ggaacctcag cacctttgag gtaacggaat
    12181 tcgagccgca taagaggtac ggattcaagt ccctcactgg tctattggat gtacaagcaa
    12241 cgtataccct cgagggagtc tttggcgaga cgaagctgaa tacctcagta caagccaatc
    12301 tgctcaattt tggccccgtg aaggaaagca ttctggaaaa gatgataaat gaacagctaa
    12361 cgaaaaattt ggaaaagctt aaatctcttc tcgaatcaag gcctcctatg cccagctctg
    12421 agcgagttta cctatcaggt gggtgatgaa tccctactct cgaggcgatg gccccgacag
    12481 ggcgtgcctg atctcaattc ttaccgttgt gagagcggac tcacgacgtg acagtcagat
    12541 gactgttttt tttcttagat aaggggggta gcaggtctgt cgcttaaggc caaagagctt
    12601 acaagcgttc ttcaccagga tatatgctcg tactttcttc tatcaaattg atcaattaaa
    12661 gatcacatcc caaaccaatc acgtcctgtt ggggagcagt tcgtccgatt ttcctgcatg
    12721 ctcaaatcgg cgcgtcgaaa tgaacgggtc ctcaactaag gtgtgtagtt cgggatgggt
    12781 tagatcacgt acccctgcac agactcgaac tgtgctctac ggctccggag gccgtcgctc
    12841 tgtccactga gctacagggg cgggttgaaa aattttatca caatcaggct ttcgccaaag
    12901 gcgccaattg cacaagttct gagcgtaact tattgatgag ctgtgtattt ccgccctcgt
    12961 caccaacctg ggcaagttgt tccttcttat gggcgagttc gcccagcacc tggtagaaac
    13021 gtcgtgtggg accaaaactt cctgagctca aggcagagag atgtgcattt gtctgaaaga
    13081 acgagatggc cgtgttatat tgttgttgag aaatcgagcc gcttgctact tcttcccgca
    13141 aattcttttt gagaatattg cgttcgtgaa tgatcatcca aatgatgaac acagacatta
    13201 aagcgtagcc aaagtaatca gcaagcaggc ccaggataaa cccgccgcca ccgccgacta
    13261 cactgctgaa ggtattgtgg aatgcatgag tcagaactgc gactgaatac cctgccggaa
    13321 atgcaaccag ctttaccagg atattctttg acatacggcc gatggccagg ccaatcccgg
    13381 tgaatgcagt gaaaaatgga tgcatccagc ccacgagcac gacgcggata aataccagaa
    13441 cccaaaaacc ttcccagcca ctttcaagaa atccatttcg atagatatat aagacgttct
    13501 cgatcgccgc gaatcccatt gcggtgatgg cgccatagac aattccatcc aggattgaat
    13561 cgaattcctt gcggaacaaa acgaacacca ccagcactgc cagcccttta agggcttctt
    13621 cgatgatggg tgcgacgatg gatgttgttc cgaattctgc cgcgccctcc gagccagtca
    13681 acgcgtatat gctgatgcca aaaagggtgt tcaaaacaaa ggcaccgcca cctgcgatga
    13741 ccactcccca aaggaatgcc gcacccagca ggagtttcgg ttccttttca taacgatcaa
    13801 gccaattcac aaacgctgcg aataagaaca tcggtacaaa cccaaagaaa aaagccccaa
    13861 gcaatgccat gatgcctccg attaacctta agaaggtcaa gactgaaaaa tcaaacgagt
    13921 tcgttcaatg cgcgtccctt cgaacgcgat gcctaacaca tccaacactt cttctggtcg
    13981 accggttgcg ccctcccgcg ccgcgagacg catgaaaact gttgttcctt gttcctctcc
    14041 ctcaggaaga gtggttaggg actcaatcaa cggacgcagg tcatatttct tcccgcgacg
    14101 ctcacggatg atggattcag attccatgag agaatcaact ttccgcttca aatcggaccc
    14161 ttcaactggt tccgttaaag taacttcata ctccgctgca atcacctgag tctgcaacga
    14221 gggtgcacgc tcctctgtgc tttccacgtt caatacctga attccggtgg gtaacgtccc
    14281 ttgaagcttt tctctcaagc cctctaaagg aatttcctgt tccagcttca tatccatcac
    14341 ttcacagcgc gaagagaatc caaggggtag tgcggcggcg atgttgatct tcggctgggg
    14401 gtgaaatccc tgtgaatacg ccaggggaag ttccgcacgg cgtgcggcgc gttcccacag
    14461 tttgtgcagg tcaagttgac cggtgtagcg caatgcgcct tgttttgcaa atgtgatgcg
    14521 tatccgcatt aagatttatt ggaattaatc gcgttcgttt tcaaccagtt ccggatatcg
    14581 cggatgtgct cctggtagtg tttataagtg ttattggcaa cacgttcgaa cagattgtgc
    14641 tgctgaatct ggtcatcaga taatttttcc agtgtagaaa tgacagagga aaacgcctgc
    14701 aattcctgcg agaccacatc ttcgagagaa acccgctgat acttttcaac aatccaggca
    14761 tttgggccat catcatccag cagggaggag ccgaagtccg ggtagccgaa ctcatccagg
    14821 aaagcatcca gggccgtctg cgttctgcac ggcgtgtatt gctcaccgtg taggatctcg
    14881 ttcatgcgat ctgcaatata ttgttcatag gcgagaatat gagccagaac atccttgatg
    14941 gaccactttc ctgatacgcc cttcaatgtc atgcggcgcg agtaacccac acgattcaag
    15001 agtaattcga acttatcacg ctcttgtagc agctttgaaa tgaattgttt ctttgtcgtc
    15061 atgctcttta tgataccctg ttctacttcc tgcttcaatg aataccaggc ttgtaaactg
    15121 atccgcaggt tataagtcta atgtccagtc taaaacctgg gcggcactcc gtcgatttct
    15181 ttt
//
LOCUS       DASTJP010000482        12266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1572891, whole genome shotgun sequence.
ACCESSION   DASTJP010000482 DASTJP010000000
VERSION     DASTJP010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12266
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572891"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFQ23_14000"
     CDS             <1..768
                     /locus_tag="VFQ23_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907759.1"
                     /translation="VLLRGKPLKMPLTAFVSQSSIKSSLPWLMLISRAVFFLVFQSLI
                     ALIFVVAGNTSAWNESARWWTIIATLANFVSIYLLVRLHNAEGRRFHDLFKFSRTEWK
                     KDLLWFLGLSIIAMPIVDAPREPLAKAIFGDSMIATTMLFRPLPTWAFVLSFLFPLTI
                     AFAELPTYFAYCMPRLSKQINNWLAWIIASFFLAAQHMFLPLIFNSGYLLWRLGTFLP
                     FALFTGLALKFRPSLLPYFLVTHALMDVATVLVYLMI"
     gene            complement(1098..1289)
                     /locus_tag="VFQ23_14005"
     CDS             complement(1098..1289)
                     /locus_tag="VFQ23_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907760.1"
                     /translation="MNQTSLTPALNDQTSMVSPGNYSSVTVDMQDILGAIFLGILSCI
                     LLIGWMRAEARIHALNKQL"
     gene            complement(1316..2227)
                     /locus_tag="VFQ23_14010"
     CDS             complement(1316..2227)
                     /locus_tag="VFQ23_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907761.1"
                     /translation="MKRLIFFLSLILIVNLACDVSVNLAPSNEVATAAETPVLRDPST
                     PTPEVFISLTQAVPATAIIQPSLSANTLVTIGRLTLEIPSSVADGASGSDIPRADEQD
                     LPTWGKTPGHTEIKLEGYTLQGKFHEPRIYVYPSLDYATLVPAAFESMHRLRNIMNPG
                     APITSDRLPNVPFFNATQVFASNLQSITFQNGSGFRFLAEYAQYFAPVNNNELVYHFQ
                     GFSADGEYYIIAILPITAPALAETSDVEAVVPAGGIAFPDIANPVVDVQTYYASITDL
                     LNATDPEAFTPPISQLDALIQSMWVAP"
     gene            complement(2227..2475)
                     /locus_tag="VFQ23_14015"
     CDS             complement(2227..2475)
                     /locus_tag="VFQ23_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907762.1"
                     /translation="MKKWIQNHQLLSFFTLTYAIAFGTTFTYIYLQPGKPFEQWSLDW
                     FLNAFSPTISALILWWIIGGWNETRRLLSGFTLWKVGE"
     gene            complement(2599..3522)
                     /locus_tag="VFQ23_14020"
     CDS             complement(2599..3522)
                     /locus_tag="VFQ23_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907763.1"
                     /translation="MYTVGSATSKTRNRPLPEKRTQSKSGTIAIRLASFIVPLALIAA
                     VAGVFWQGTGEPYPFETLRGETVMIRGYGLYRYDTVNSSSQEIATDIVTLLIGIPLLI
                     TGIFLSRQGSLRGQLLLTGALGYFLYTYGAMCFLTAFNPLFLVYVALFSLSLFGFILS
                     ISNLDVEEIARHISSGFPRRAVAAYFMIVAIFLTLAWIGLVASPSLTWSPPVGLESAI
                     TMVIQALDLGVIVPTSFITATLLFRKLPWGYALSSVLLLKILTMGAALIAMIIAQRLA
                     GVPIDPITSTIFIIISLSGITLGIISLKNIR"
     gene            complement(3541..4419)
                     /locus_tag="VFQ23_14025"
     CDS             complement(3541..4419)
                     /locus_tag="VFQ23_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907764.1"
                     /translation="MKHYPALNWLVAIISICALVAVGFGLFTPSNGSLISFRTIREET
                     VEIWGQGLYKYDTPIGAIGFTAADLITLVLAIPILLVSIRMYRKGSLKGGLLLSGVLA
                     YFLYNYTSMGFGTAYNNFFLTYTVIFSASFFGLILTLISFDLKSLPAHFKSGLPRNGM
                     GVFLIVSGVILSLIWLLLSILPALIADKAPPEAYYYTTFTTGIIDIGIVSPALILAGV
                     LIRRGAPLGYLLASAMLIFTCILGANLSAAGVIQVVKEVITVGQAMAFTVPFVMLSLI
                     AVWFSVLLFRNVSDTV"
     gene            complement(4448..4939)
                     /locus_tag="VFQ23_14030"
     CDS             complement(4448..4939)
                     /locus_tag="VFQ23_14030"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9907765.1"
                     /translation="MSKLDDRVQDFLAQKKIAVVGVSDQRDTGCNLAYKRFKESGYQV
                     YPVNPRITAYDGATCYPDLKSIPETPDAVFILASPRVTEQIVQQCVDLGIHRVWMHCM
                     MGTRPGLASGMTSVSEDAVRLCRLNGITVIPGGCPNQFLKPDLGHALMRTMWRTLGFY
                     ELN"
     gene            complement(5127..5588)
                     /locus_tag="VFQ23_14035"
     CDS             complement(5127..5588)
                     /locus_tag="VFQ23_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907766.1"
                     /translation="MSSLLISLSSWLHTIGTIVFIGYYLLLSLIFIPALKNERTSILS
                     EISKRSRLWMYVSLLIFALTGSYLTLVDSNYLGLANFGNFWGILMLVKHLLILGMIGM
                     GFWFNAILRVGPMMRSNSGAEQARQRFRLYVNLMAISGVLVLLLTALAQVE"
     gene            complement(5591..6268)
                     /locus_tag="VFQ23_14040"
     CDS             complement(5591..6268)
                     /locus_tag="VFQ23_14040"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET9907767.1"
                     /translation="MKKPLLLCGMFASLVYVGTVILGGLIRPGYSHLSEAISELVADG
                     APNRSLLSSLFLVYNVLLSAFGIGLFFKAKERRRARIMGSIGSWTLILVGMAGISMEL
                     VFPQDPGGIPTTVAGTMHLVMAGFASLGTMIAVLLLALWFRNFGALSDLVAYSWFSVV
                     IIFLSGGLTAAAMVNYYPLFGVIERVTIGTFILWLFVISRRMLQLENIASRHTHVEMV
                     GSVPKGR"
     gene            complement(6378..6938)
                     /locus_tag="VFQ23_14045"
     CDS             complement(6378..6938)
                     /locus_tag="VFQ23_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907768.1"
                     /translation="MKENQMTLASLTLKTIVAHTLTYFIVGLIAFNLFDYAAQFAEDE
                     LRTFMRQTDDPMVALGPALQPIRGILFALAFYPLREILFKRKNGWLITWWLLVSLGIL
                     STFAPGSGSVEGIIYTTLPIAGQFSGGMLEILTQSFLFSALLYYWVNNPEKRWINWVL
                     GILFVLAVLMSVMGYLIASGAIVVPA"
     gene            complement(6950..8440)
                     /gene="crtI"
                     /locus_tag="VFQ23_14050"
     CDS             complement(6950..8440)
                     /gene="crtI"
                     /locus_tag="VFQ23_14050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HET9907769.1"
                     /translation="MKPKSVIVIGAGVGGIAAAAHLARHGLRVTVFEKNPLPGGRCNR
                     FSRDGHHFDTGPTLLIMPMIYEAEFAALGASLHEMLDLHRVDPSYHLIFDDGHRLELT
                     SDMNKMHEQLEAFEPGSFGGFLRYLDEGFQHYRLGVEKLVNHDFRRASDFFRLDNLPL
                     FYQLKPLVNHYQHMSAYFDDPRLKAAFTFQDVYMGLSPFEAPATFSMMSYTELVHGVW
                     YPKGGMYSVVQALMDLAQRAGVEFQFDAQVDRIEVDENQARRIILTDGRPVTADVILA
                     NADLPYVYKNLLPKDRETERLVHKKYSCSVISFFWGLDQSYEQLNPHTLFLADDFSEN
                     FDSIIQELELPVNPSLYIHAPARLDSSMAPTGQDTIIAIVPVGHLGEKETQQWGEIQD
                     CARQHVFRRLSAIGITDIQDHLKFEVSYSPLSWLKRYNLVKGSTHGLSHNLMQLGYFR
                     PHNRHAQYLNLYFVGASTHPGTGIPTALVSGRLAAQRILDDLHRLN"
     gene            complement(8437..9357)
                     /locus_tag="VFQ23_14055"
     CDS             complement(8437..9357)
                     /locus_tag="VFQ23_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HET9907770.1"
                     /translation="MLITPAAVITKAASKQAYYTIRFLADRERADDAYRAYGYFRWVD
                     DIVDSGFDSNSERSAFLERQKVLLDGCYRGERMQAATAEENMLIQLVQHDHEKCSGLQ
                     VYLRNMMKVMQFDAGRRGRLISQVELDRYTHQLSCAVTEAMHYFIGHDDFAPHTQSRY
                     LAVAGAHIVHMLRDAYEDLQMGYYNIPREVLESGQIRPQDIQSRAYRAWVQSRIQLAR
                     MYFRAGKRQIAQIENMRCRLAGYAYVARFEWVLNTIEREAYVLRPDYSDRKSFKAKYR
                     MGLLAVSSIFNSKSRLLIYQPARSQLWRKR"
     gene            complement(9717..10139)
                     /locus_tag="VFQ23_14060"
     CDS             complement(9717..10139)
                     /locus_tag="VFQ23_14060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET9907771.1"
                     /translation="MSLPIPESHFDLITRPVHGVLTTMMPSGQPQSSLVWCDYDGECV
                     CINTTRERQKGRNMLHNPKVSLLIVDPENTGRYLELRAEVEITTENVLEQLDRVTRKY
                     TRHPQFYGYVYPLEQMERETRIICRIHPHKITMDAIHK"
     gene            complement(10120..10569)
                     /locus_tag="VFQ23_14065"
     CDS             complement(10120..10569)
                     /locus_tag="VFQ23_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9907772.1"
                     /translation="MNKIPDAFLDLVNCPRVAALTTVMPDGQPQTTVVWCDFDGRYVR
                     VNTMRGFRKEKNMRVNSRVTLLCFDPCEPLRSLEVRGIVIKMTERGALEHLDSLSERY
                     VGRKPYFGNCVPVEFKAKEIPVLCKILPMHVVTLDARKEKAHEPANP"
     gene            complement(10562..11191)
                     /locus_tag="VFQ23_14070"
     CDS             complement(10562..11191)
                     /locus_tag="VFQ23_14070"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET9907773.1"
                     /translation="MEFLINPNFAYVLIVTGVMLSLWTFNEPKSTRQKAGMVVCFVAV
                     GYELFYLNWNPLALLIVAISPLPLFLAIRQARFQSPLFLLTILMLTMGSVFLFVDRNN
                     LPVVNYGLAAIVSVFCATFIWIGIGRMRNADGIRPSTDPDSVVGLMGVVQREIELYSA
                     GSVLVDGELWQARSKKPIPAGATVRVLRQDGFWLTVKEVQKLLQKNEDE"
     gene            complement(11205..11519)
                     /locus_tag="VFQ23_14075"
     CDS             complement(11205..11519)
                     /locus_tag="VFQ23_14075"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HET9907774.1"
                     /translation="MKQNDSLFGPLFLIAAGVIWLLIKSGNIPASNLWALTHSWPFLL
                     IAAGLGLLLKPYWQYTSLVLDVIIIGGLILAIVYAPALGWDRPSSIFVISAIVCTGRA
                     AF"
     gene            complement(11540..12097)
                     /locus_tag="VFQ23_14080"
     CDS             complement(11540..12097)
                     /locus_tag="VFQ23_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907775.1"
                     /translation="MQTNRPNNAALIGGVLLIAFGLMSLAVRFLRDVGWGYLWSFAVI
                     GLGALFFVAMLAGGKQFAALAIPGSFISGIGLILLFQIITGHWELMTYFWTLILLFIG
                     LGIYIMGIYAVDENQRQSGLRVMKAALILFLIFGTFFEMIFSSFNHTLFPMLLIGLGA
                     YIVLKRTGLFGRESKHSSDSLTHPT"
BASE COUNT         3184 a         3132 c         3050 g         2900 t
ORIGIN      
        1 gtcctgctcc ggggtaaacc tctcaagatg ccgctcacag cctttgtttc acaatcaagc
       61 attaagtctt cacttccctg gctcatgctc atctcgcgcg ctgtgttctt cctggtcttt
      121 caaagcttga tcgcgttgat cttcgtcgta gcgggcaaca cctctgcctg gaatgaatcc
      181 gcgcgctggt ggacgatcat agcaaccctg gccaacttcg tgtccattta tctgctcgta
      241 cgtttgcaca atgccgaagg cagacgtttc catgatctct tcaaattttc gcgcaccgaa
      301 tggaaaaaag acctgctctg gttccttggg ttaagcatca tcgccatgcc catcgtcgac
      361 gcgccgcgtg agcctcttgc caaggcgatc ttcggtgatt ccatgatcgc cacgaccatg
      421 ctcttccgcc ctctgccaac ctgggcattc gtgctaagct ttctcttccc actgaccatt
      481 gcctttgccg aactgccaac ctattttgca tactgtatgc cgcgtctcag caaacaaatc
      541 aacaattggc tggcttggat catcgcctcc ttcttcctcg ccgcgcagca catgttcctg
      601 ccgcttatct tcaatagcgg atacttgtta tggcgactgg gaacgttcct gccctttgct
      661 ttattcaccg ggcttgcctt gaaattccgc ccttcgctct tgccctactt tttggttacc
      721 cacgcgttga tggatgtcgc gacagtgctg gtctatttga tgatataggc agtttcactt
      781 ttaccagcac ggattccaat ccggcaagtt catgtattgg agtattctct tgcaagccat
      841 tccccgtctt caagtttgcc gctggcctga tgaggaacct gaagtgtatt ctgaacaggc
      901 tcagtgtatg ttcccgatac gctttgaata tcgcgttggt cactgattaa ctcaaaccct
      961 ttcgccaccc gttagggcag gtgaaacaca gctgatttct tactaacccg gcatctgtcc
     1021 tcgtcgcgat tcccgaaccg gaattcacat ggtacgaatt agaagatgag caattaacat
     1081 atctgaagcc aggttggcta caattgtttg ttcaatgcgt gaatacgtgc ctctgcgcgc
     1141 atccagccga tcaacaagat gcaggaaagg atgcccagga atatggcgcc aaggatatct
     1201 tgcatgtcca cagtcaccga gctgtaattg cccgggctga ccatggaggt ctgatcattc
     1261 agagccggtg taagtgaggt ttggttcata gcatcctcct gttagagtca atggtttaag
     1321 gagcgaccca catggactga atcaacgcgt ccaattggct gatcggggga gtgaaagctt
     1381 cggggtccgt ggcattcaac aggtctgtaa tcgaggcata ataggtttgc acatccacaa
     1441 cgggatttgc aatatcggga aaagcgattc cgcccgcagg gacaactgct tccacatcac
     1501 tggtctctgc cagggctggc gcagtgatcg gcaaaatcgc gatgatgtaa tattcaccat
     1561 cggcgctgaa gccctgaaag tgatacacca gttcattgtt gttgaccggc gcgaaatatt
     1621 gagcatactc agccagaaag cggaagccgc tcccattttg gaaggtgatg ctttgaaggt
     1681 tcgatgcgaa tacctgggtg gcattgaaga atgggacatt ggggagtcga tcggaagtga
     1741 tcggcgcgcc aggattcatg atgttgcgaa gacgatgcat actttcaaat gcggcgggga
     1801 ccaatgttgc ataatccaac gacggataca catatatcct gggctcatga aatttccctt
     1861 gcagggtgta gccctcaagt ttgatttccg tgtgaccggg agttttcccc caagtgggaa
     1921 ggtcctgttc atcggcgcgc gggatgtcgc tgccacttgc gccgtcggca acgctcgatg
     1981 gtatttcaag agtcaagcgc ccgattgtga caagggtatt tgcactcaga gatggctgaa
     2041 tgatagccgt cgctggaact gcctgtgtca atgagatgaa aacctcaggc gtaggtgtgg
     2101 aagggtccct caaaacggga gtttccgcag cagtggcaac ttcgttgctg ggcgcaaggt
     2161 tcaccgaaac atcacacgcc agattcacga tgaggatcag agagaggaaa aagattagtc
     2221 ttttcattac tcacccacct tccagagggt aaaccctgag agcaaacgcc tggtctcatt
     2281 ccatccgccg atgatccacc agaggatcaa cgcgcttatt gtggggctga aggcattcag
     2341 gaaccagtcc agcgaccact gttcgaacgg ctttcccggt tgcaggtaaa tataggtaaa
     2401 tgttgtgcca aacgcgatgg cgtaggtcaa agtgaagaac gatagtagct gatgattttg
     2461 aatccacttc ttcatggcag actcctcatt gaggtttgat cagctctact gaagattggc
     2521 cgccaccagc aaaatgccgc ggatgatgat caaagttgtt ttcgctgatt tgtccgtagg
     2581 aatctccttt cggaatcact atcgaatatt tttcaggctg ataattccca gggtgatgcc
     2641 ggaaaggctg atgattatga agatcgtgga agtgatggga tcgatgggaa ctcctgctaa
     2701 tcgttgagca attatcatgg cgatcagcgc cgcgcccatc gtcagtattt tgaggagcag
     2761 cacagaggag agcgcataac cccagggaag tttcctgaag agtaaagtgg cagtgatgaa
     2821 actcgtcgga acgattacac ccaggtcgag cgcctgaatc accattgtga tcgcggattc
     2881 caggcccacc ggtggggacc atgtcagcga gggagaggca actaatccga tccaggcgag
     2941 ggtgagaaag attgctacga tcatgaagta cgcggcgacc gcgcggcgtg gaaatccgct
     3001 tgagatgtga cgggcgatct cttctacatc cagattactt atggagagaa taaatccgaa
     3061 gagactcaac gagaacagcg caacataaac aaggaataac ggattgaatg ccgtgaggaa
     3121 acacatcgcg ccataggtat acaggaagta gcccagtgca cctgtaagca gaagttgacc
     3181 gcgcaaactc ccctggcggc tcaggaagat gccggtaatg agcaggggaa taccgatcag
     3241 taatgtgaca atatctgtcg cgatctcttg agaggaggag ttaaccgtat catatcgata
     3301 caacccatat ccacggatca tgacagtttc gccgcgcaag gtttcaaatg gatacggttc
     3361 gccggttccc tgccagaaga cacctgcaac ggccgcaatc aatgcaagcg gtacgataaa
     3421 agatgctaag cggatcgcaa tcgttccgga tttcgattgc gttctttttt ccggcaaagg
     3481 ccggttgcgg gttttgcttg ttgcagaacc gactgtatac atggaatact cccttctttg
     3541 ttaaaccgta tctgatacgt tccggaaaag cagcaccgag aaccagacag cgatcaatga
     3601 cagcatcaca aatggaacag tgaatgccat ggcttgaccg actgtgatca cttccttcac
     3661 cacctggata accccagctg cagagaggtt ggctcccaaa atacaagtga agatcaacat
     3721 tgcggaggcg agcagataac ccaatggcgc gccgcgccgg ataagaactc ccgccaggat
     3781 cagcgcggga gaaacaattc caatgtcgat gatccctgtg gtgaatgttg tgtagtaata
     3841 cgcttcgggt ggtgctttgt ctgcaatcaa ggccgggagg atggacagca gcagccatat
     3901 caaactgagt atgacgcctg acacgatcag gaagacaccc attccattac gcggcaagcc
     3961 agacttaaag tgtgcaggca gactcttcag gtcgaaggag atcagggtca ggatcaatcc
     4021 gaaaaagctg gctgaaaaga taacggtata cgtcaggaag aagttgttat aggcggtgcc
     4081 aaagcccatc gatgtgtagt tgtaaaggaa atacgcaagc acaccgctca ataacaatcc
     4141 acctttgagc gaacccttgc gatacatgcg gatggaaacc aatagaattg gaatggcaag
     4201 aacgagggtg atcaaatctg cggctgtaaa gcctatggcg ccgatcggtg tatcatattt
     4261 gtatagccct tgtccccaaa tttctaccgt ctcctcgcgg atcgtgcgga acgagatcag
     4321 gctgccgttg cttggtgtaa agagcccgaa gcccacggcg acaagcgcgc aaatcgaaat
     4381 gatcgcgacc agccagttga gcgcgggata atgtttcatc ggttctctac gacttttttc
     4441 tttgacctca atttagttcg taaaagccca gggtccgcca catcgttcgc atcaacgcgt
     4501 gaccgagatc cggcttcagg aattgatttg gacagccgcc cggaatgact gtgattccgt
     4561 tcaatcgaca taaacgtacc gcgtcttcag agacgctcgt catgcctgac gccaatccgg
     4621 gcctcgtccc catcatacaa tgcatccaga cacgatgaat tccaagatcc acacactgct
     4681 ggacgatctg ttctgttact ctggggctgg caagaatgaa gacggcgtct ggtgtttcgg
     4741 gaatggattt gagatccggg taacatgtag caccatcata ggccgtgata cggggattca
     4801 ccggatacac ttgatatcca ctctctttaa atctcttgta tgccagattg catcccgtat
     4861 cgcgctgatc tgagacacct accactgcga tttttttttg agccagaaaa tcttgtacac
     4921 gatcatcaag tttggacatt ggaactcctt tccatttgct tttgtcttaa ttggaagcta
     4981 caaaacctgt tcagccaatt tccttgaaaa agacggcgat aagtccgatc tcgattccaa
     5041 tcactgagaa ggctggagaa gattttcata aatggctgtc ctttggggct tgggaacttc
     5101 aggttcgtca caattgtttc atctacttat tcgacctgtg ccaaggccgt cagaagtaag
     5161 accaacacgc cagagatggc catcaggtta acatacaggc gaaagcgttg acgcgcctgt
     5221 tcagctccgc tgttcgaacg catcatcggt cccacgcgca ggatggcgtt gaaccaaaag
     5281 cccatcccga tcattcccag gatcagaaga tgctttacca gcatcaggat tccccagaag
     5341 tttccgaagt ttgcaagtcc taaataattg gaatccacaa gcgtgagata gctgccggtc
     5401 agcgcgaata tcaatagaga tacatacatc cagagtcggc ttcgctttga gatttcactg
     5461 agaatggatg tacgctcatt cttcagagcc gggatgaaaa taagagagag caatagataa
     5521 tagccaatga aaacgattgt cccgatggta tgcagccaac tggaaagaga gatcaatagg
     5581 gatgacatcg ttatctgcct tttggtactg agccaaccat ctctacatgc gtgtggcgcg
     5641 aagcgatatt ttccagttgc agcatcctgc gactgataac aaacaaccat aggatgaagg
     5701 tgcctatggt gacgcgttcg atcactccaa agagcgggta ataatttacc attgcggcag
     5761 cagtcagccc acctgaaagg aagataatta cgacgctgaa ccaggagtag gcaactaaat
     5821 cactcagggc gccgaaattc ctgaaccata gtgcaagtaa cagcacggca atcattgttc
     5881 ccagggatgc aaatcctgcc atgacgaggt gcatggttcc ggcaacggtt gtagggattc
     5941 ctcccggatc ctgcgggaat acaagctcca tggaaatgcc cgccataccg actaggatta
     6001 aagtccacga tccaatggag cccatgatcc gtgcacggcg ccgctctttt gctttaaaga
     6061 acaagccaat tccaaaggcg cttaatagca cgttgtacac cagaaacaag gaactaagca
     6121 gcgaccggtt cggcgcgcca tctgcgacca gttcactgat cgcttcagat aaatgactgt
     6181 agccgggccg gatcaaaccg ccgagaatta cggttcccac ataaacgaga gatgcaaaca
     6241 ttccgcagag cagaagaggc tttttcattt tgagaaattt cctgcttgga gtttagcgga
     6301 ggagataaaa agactcccct gccacgaggg gagtcttcac tatccattcg gacagtgtgg
     6361 ttcggaggat gggtggctta ggctggcact acgatggcgc cgcttgcgat caaatatccc
     6421 atcaccgaca tcagcacagc caatacaaaa aggatgccca atacccagtt gatccagcgc
     6481 ttttccgggt tgtttaccca gtagtagaga agcgcagaga agaggaagga ttgagttaag
     6541 atttccaaca tgccgcctga gaattgtcca gcgatcggca gagtagtata gataatgcct
     6601 tccacagaac cggaccctgg agcaaaggtg gaaaggattc ccaaagagac gagcagccac
     6661 catgtgatca gccagccatt ctttctctta aacagaattt ctctcaacgg ataaaaagcc
     6721 agcgcaaaca ggatgccgcg aatgggctgg agtgccggtc ctagcgcgac cattggatca
     6781 tcggtctgtc gcataaacgt tcttaactca tcttccgcaa actgcgcggc ataatcgaag
     6841 aggttgaagg cgatgagccc cacaatgaaa tacgtgagcg tatgcgctac gatggttttg
     6901 agcgtcaagc tcgctaatgt catctgattc tctttcattc tgactccttt cagttcaaac
     6961 gatgaaggtc atccagaatc cgttgcgccg caagacgacc cgacacaagc gcggttggaa
     7021 tacctgttcc cgggtgtgtg ctcgcaccca caaaatagag attaagatac tgtgcatgcc
     7081 ggttatgagg acggaaataa cccaattgca tcaggttgtg ggaaagccca tgtgtagagc
     7141 ccttcaccag attatagcgt ttcaaccagg ataggggcga atagctgact tcaaatttga
     7201 ggtggtcctg gatatcagtg atgccgatcg cgctcaagcg gcgaaagaca tgctgacgcg
     7261 cgcaatcctg aatctctccc cattgctgtg tctccttttc acccaggtgt cccacgggca
     7321 caatcgcgat gatggtatcc tgccctgtgg gagccatgga ggagtccagt ctggctggag
     7381 cgtggatata aagactgggg ttaacaggca gttcgagctc ctgaatgatg ctatcgaaat
     7441 tctcgctgaa atcatccgca agaaatagcg tatgagggtt caattgctca tacgattgat
     7501 ccaagcccca gaagaagctg atcactgaac aggaatattt tttatgaacc agccgttctg
     7561 tttcgcgatc cttaggtaat agattcttat agacataagg aagatccgca ttggccagaa
     7621 taacatcagc tgtgacagga cgaccatcgg tcaggatgat acgccgggct tgattctcgt
     7681 ctacttcgat tcgatctacc tgcgcgtcaa actgaaattc cacaccggca cgttgagcca
     7741 gatccatcag cgcctgtaca acagaataca tccctccttt ggggtaccag acgccgtgga
     7801 ccaattctgt gtaagacatc attgaaaatg tagctggagc ctcaaaggga cttagcccca
     7861 tgtatacatc ctgaaatgtg aatgctgcct ttaaacgtgg atcatcgaaa taagcagaca
     7921 tatgctggta atgattgacc agcggcttca actggtaaaa caatggcagg ttgtcaagac
     7981 gaaagaaatc agacgccctt ctgaaatcat ggttgaccaa tttctcgacc cccaaacgat
     8041 aatgctggaa accttcatcc aagtagcgca aaaatccgcc aaagctgcca ggctcaaaag
     8101 cttccagttg ctcgtgcatt ttattcatat cagaagtcag ttccaggcgg tgcccgtcgt
     8161 caaagatcag gtgatagctc gggtcgacgc gatgaagatc cagcatttca tgcagcgagg
     8221 cgcccaacgc cgcgaactcc gcttcataga tcattggcat aataagcaga gttggacctg
     8281 tgtcaaagtg atgcccatcg cggctgaagc ggttgcagcg tccgccgggg agcgggttct
     8341 tctcaaacac agtgaccctt aaaccatgac gcgccaggtg agccgccgcc gcgattccac
     8401 ccactcccgc accaatgaca atgacagatt ttggcttcat cgcttcctcc ataattgtga
     8461 acgcgcaggc tggtagatca gaagcctgct tttcgaattg aatatcgaag aaactgccag
     8521 caatcccatt cggtatttgg ctttgaaact tttccggtca ctgtaatctg gccgcagaac
     8581 gtacgcttca cgttcgatgg tgttcaatac ccattcgaag cgtgctacat aagcatagcc
     8641 tgccagtctg cagcgcatgt tctcgatctg ggcaatttgc cttttccctg ccctgaaata
     8701 catccgcgcc aattggatgc gactctgaac ccaggcgcga taggccctgc tttgaatatc
     8761 ctgtggtctg atctgcccgg actccagcac ttcacgtggg atgttgtagt atcccatttg
     8821 aaggtcttca tacgcatctc gcaacatatg tacgatgtgc gcgcctgcca ccgccagata
     8881 acgcgactga gtatgtggtg caaaatcatc gtgcccgata aagtaatgca tcgcttcagt
     8941 cacggcacag gaaagctggt gtgtgtatcg atctaattcg acttgtgaaa tcagcctgcc
     9001 tcgccgaccc gcatcgaatt gcatcacctt catcatattg cgtaaataga cctgtagtcc
     9061 actgcatttc tcatgatcgt gctgaaccaa ctggatcagc atgttctcct cggctgtcgc
     9121 ggcctgcatc cgctctcccc gatagcagcc atccagcaaa actttttgcc gttcgagaaa
     9181 ggcacttcgc tcggagttcg agtcgaaacc tgagtcgacg atgtcgtcta cccagcggaa
     9241 gtagccatag gcccgatagg catcgtcggc ccgttcgcga tcagccagga atcggattgt
     9301 gtaataggct tgtttgctgg cagccttcgt aatcactgcg gcgggcgtta tgagcatctg
     9361 attcaccctg ttatcctttc cgggataccg tgcttgatct tcgttactct ctcagactca
     9421 ctgatcgagg ttaatacgat gggcattgcc atagagtctg atcgtatacc gcagcctggg
     9481 cagaccgggc gcgctgggaa gattcgagaa ccgcagtttc ggcaaattga accttccagg
     9541 cggtaacgtt gtgctttcaa tctccagtga caagggattt ccatacgaat tattcactcg
     9601 tgttccagcc ccatcatata tccggtaaac aaaagactcc tcagcctgca gaggagtctt
     9661 cactatccac tcggacagtg tgatcagatg gattctatta ttccccgctt cgtttcctat
     9721 ttgtggatgg catccattgt tatcttgtgt gggtgaatac ggcagatgat gcgcgtttcc
     9781 ctttccattt gctcgagcgg atagacataa ccataaaact gaggatgacg ggtatatttc
     9841 cgcgtaacac ggtccagctg ttccagtaca ttctccgtag tgatctccac ttcagcgcgc
     9901 agctcgaggt aacggcctgt gttctccgga tccacgatga gcagggatac tttcgggttg
     9961 tggagcatat tcctgccctt ttgacgttcc cgagtcgtat tgatgcagac acactcacca
    10021 tcatagtcgc accacaccag gctggattga ggctgaccac tcggcatcat tgtggtgagg
    10081 acgccatgaa cagggcgcgt gatgagatca aaatgactct cagggattgg caggctcatg
    10141 ggctttctcc ttgcgcgcgt ccagcgtgac cacatgcata ggcaggatct tgcacaacac
    10201 agggatctcc tttgctttga attccacagg aacacaattt ccaaagtaag gcttcctgcc
    10261 cacgtaacgc tccgagaggc tatccaggtg ttccagtgcg ccgcgctctg tcattttgat
    10321 gacgatgccg cgcacttcaa gagagcgtaa cggctcgcat gggtcgaagc agagcaaggt
    10381 cactctcgaa ttgacacgca tattcttctc tttgcgaaag ccacgcatcg tattgacgcg
    10441 tacgtaccgt ccatcgaaat cacaccacac gacggtggtc tgaggctgcc catcgggcat
    10501 aacggtcgtc aacgcggcaa cacgcggaca atttacaagg tcaaggaaag catcgggaat
    10561 tttattcatc ttcgtttttt tggagcagtt tctgcacttc tttgacggtc aaccaaaaac
    10621 catcctgtct taacacgcgt accgttgcac cagcagggat cggtttttta cttcgggcct
    10681 gccacagttc tccatccacc aagaccgagc cagcggagta cagctcaatc tctctctgaa
    10741 caactcccat gagtcccacg acggagtcag ggtcagtgct tggccgtata ccgtccgcgt
    10801 ttcgcatccg accgattcca atccagataa acgtggcgca gaatacagaa acgattgcag
    10861 cgagaccata gttaaccact ggaaggttgt tcctatctac gaacaggaaa acggagccca
    10921 ttgtgagcat caagatggtg aggagaaaga gagggctttg aaaacgggcc tgccggatcg
    10981 cgagaaacag cggcagtggg ctaattgcaa cgatcagcaa cgccagggga ttccaattga
    11041 gatagaacaa ctcgtacccg accgcgacaa aacaaactac cattccagct ttctgcctgg
    11101 tagattttgg ctcattgaat gtccacaacg acagcatcac accagtgaca atcagtacat
    11161 aggcgaaatt cgggttgatc agaaattcca ttttctcctc cgtatcagaa tgcagcccgg
    11221 cccgtacaaa ctattgcgct gatcacaaag atagaagatg gacgatccca gcctagagcg
    11281 ggtgcataaa cgatggccag tatcaatcca ccaatgatga tcacatccaa aaccagggag
    11341 gtatattgcc agtagggttt cagcagaagc cccagtccgg ctgcgatgag cagaaagggc
    11401 caactatgag tcaacgccca aagatttgaa gcggggatat ttcctgattt gatcaacaac
    11461 cagattacac cagctgcaat cagaaacaaa ggaccgaaga gagaatcatt ttgtttcatg
    11521 attattttac ctttcaatat tatgttggat gtgtgagtga atcagatgaa tgcttactct
    11581 cacgtccgaa caggccagtt cgtttcagga cgatatacgc gcccaggcct atcaaaagca
    11641 ttggaaagag ggtgtggttg aaggatgaaa agatcatctc gaaaaacgtg ccgaagatga
    11701 ggaaaagaat caatgcggct ttcatcacac gcaatccaga ttgcctttga ttttcgtcaa
    11761 cagcatagat gcccatgatg tagatgccca ggccgatgaa caggaggatt aaggtccaga
    11821 agtaggtcat caattcccaa tgtcccgtga tgatttggaa caacaaaatc aaaccgattc
    11881 cactgatgaa acttccaggg atggcaagcg ccgcgaattg tttcccaccc gccagcatgg
    11941 caacgaaaaa cagcgcgccc aaaccgatca cagcgaaaga ccaaaggtaa ccccagccca
    12001 cgtctcgcag gaacctcacc gcgagagaca tcagaccgaa ggcgattaaa agcacacccc
    12061 cgatcagggc ggcattgtta ggacgattgg tttgcatttt ttcattctcc tttcagatgg
    12121 gtcttatgag attgaacaac ttaacgattc ctgattgatc accgacatca gcctgttcag
    12181 gatcgtatct ggcgccgcgc ccttgtgaat gaaacctgca ccggcgttcc tggcgatcga
    12241 ttcgtcattt gcatccaggc tgagca
//
LOCUS       DASTJP010000483         4863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_20555, whole genome shotgun sequence.
ACCESSION   DASTJP010000483 DASTJP010000000
VERSION     DASTJP010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20555"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFQ23_14085"
     CDS             <1..279
                     /locus_tag="VFQ23_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907776.1"
                     /translation="ELGNTCQEMQFNDDQITEVCTAYFGNATADKIARMKLNMIMSDV
                     GWGLWAAIQAKISTIDFDFWGWALERWGRAVEKMDSREFEGWIKDIQA"
     gene            464..2481
                     /locus_tag="VFQ23_14090"
                     /pseudo
     CDS             464..2481
                     /locus_tag="VFQ23_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2629..2883
                     /locus_tag="VFQ23_14095"
     CDS             2629..2883
                     /locus_tag="VFQ23_14095"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET9907777.1"
                     /translation="MVLDNDEYTQIYGGEAVYHEGRVLTRIRSGGYGYTLKKNILYAY
                     LPMELSKPGSRVEVELIEGRYIAEVTAAVLYDPKSEKLRA"
     gene            2932..4029
                     /locus_tag="VFQ23_14100"
     CDS             2932..4029
                     /locus_tag="VFQ23_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET9907778.1"
                     /translation="MPKSAPDYVGFGMLTPVEIMVLEKFPRLNTGAIVKDVNQFIFDD
                     AAIVACLLRQWEVSAGIIGTAVGDDLRGRALAKQLKEWGVQGKVRFTNEYKTPIEVNV
                     SDRRGARTYFWQRTPQILKTLDTADLSLLRGAKIMYADWYDGDHILRAMDEAKRLDVP
                     VFVNLEHGHRKAETLKKFAERATICQAVTDAAQLGGKQALVGTAKKLLKSGIQTAIIT
                     LAKGGCLVAQGDEIVRVFAPQVRAVDASGAGATFSAGFIYGYLKGWNLEASARFATAA
                     STLKVTRAGLQMFPVEQILGLAAVLKVEHWAFRNNRFEIIDRILSIPRQMADQGKRAQ
                     QKILRELRFPKKQPGPRNVETGQSKTRRKTV"
     gene            4121..>4863
                     /locus_tag="VFQ23_14105"
     CDS             4121..>4863
                     /locus_tag="VFQ23_14105"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HET9907779.1"
                     /translation="MSFAELLTQEQVERVHEASLEVLQEVGLKVRYAPAREVFAQHGA
                     KTEADSEFIKLPRDVVEKYRKMAPPKFTFRGRDPKFDKTIPDDSPVIVTASSAPDIID
                     PVTGKERRAESGDIARIAHLINELPGYDMFSISTLADDAPRDQFTISRLYPALKYCLK
                     PIRVTTTDHKDTLAMMEMAYIVAGGKDAYMEHPFLTHHYCPTVSPLSMDVLSTENVMY
                     FAGEGLPVYPTIVPNAGLTSPMSMAGTLV"
BASE COUNT         1268 a         1143 c         1322 g         1130 t
ORIGIN      
        1 gagttgggca atacgtgcca ggaaatgcaa ttcaacgatg atcaaatcac tgaagtatgt
       61 acggcatact ttggcaatgc tactgccgat aaaattgcgc gcatgaagtt aaatatgatc
      121 atgtcagacg tgggttgggg attgtgggcc gcgattcagg cgaagatatc cacaattgat
      181 tttgacttct ggggttgggc cctcgagcgc tggggcaggg ctgtggagaa gatggattca
      241 agagagtttg aagggtggat aaaagatatt caagcataat cggcggttga ttagccctga
      301 gcgtttattg cgaagagcgt acacttgcac cgcacggcgt ttgttgcctg gcaggtgtcg
      361 aaatcaccag gttgcaagga aatctcatat ttttacgtaa aggttggttt cgatacggcc
      421 gaagaacacc gcctactcaa ccaacggcga taaaggtaac cgaatgaaat ctgaagcaga
      481 aatcgtagtg atcggcgggg ggatttatgg agtaagcgtt gcgtatcatt tattcaagtt
      541 ggggaaaaaa gacattgtcc ttttggaaaa gagtgatatt gccagtggag agtcggcgca
      601 cgcggcaggc gtggtgaccc agtttgccac gtctaaaacc atgatgcaat ttcggaaata
      661 ctccatcgag ttgtggagtg aactgggttt gttcaatcat gttgggagtt tgcgggtcgc
      721 ctccagcaaa gagcagctta aggagttaga acgaagtgtg agcagggcaa aggccatcgg
      781 catggaggtg gagatcatat cgcctgccga agctctgaaa gtcatgccgc agatcacgga
      841 taaagattta tacggtgcaa tctatctgcc acgcgatggg catctgattc catatgacac
      901 tacaacggca accgcacggc tcattaaaga gatgggggtg gaagtcaaca cacatacccg
      961 cgtcaccggg tttgaactct caccaaaggg tgagattcaa gcggtgctca cggacaaagg
     1021 aaaaatcaaa acgaatattg taatcaacgc ggccggtatg tgggcgccgc gcatcgctgc
     1081 gatggttggt gtgactttgc ctaccactcc ggttgaccac cagcacatca cacttcaagc
     1141 ggttccgggg catgagttct cgaagaatac gccatgtttg cgcgacccag ataacctggt
     1201 ttatatgcgc gaagagcagg gcgggcttgt cattggtggc tatgagccag acccgccggc
     1261 gcgctggatt gacggtacac cttgggaaca tgccaataca actgtgcctc atgattatca
     1321 tcgctttgaa atgctgctcg agggcgcgat ccgccgtata ccgttcctga aggatgccgg
     1381 catcattgac atcacctgtc accccggtgc gtatacgcct gactgtcagc ccctgctggg
     1441 accgatccct ggtgtgcgcg gcatgtggat gctggccggc atctcgctca acgggtatgg
     1501 tggcgcgggc gggatgggca aactgctggc agagtggatt atcgaaggcg aagcagcaat
     1561 ggacgtaaat gcatttcgcg ccacgcgctt cgggaattac tttactgatc cgcaatatgc
     1621 tgctgagcgc acgcgggaga gcgtcaaata ttattatgcg atccgcttcc ccaacgatga
     1681 aaatgaatgg gcgcgtccac atcgaatgag cccagtctat catcgtactc aggaactcgg
     1741 agcggtgttc ggagaaaaat ttggctggga gcgtgttctg tattatcaac caggcgagac
     1801 atcgcggcgc gcaggagcag atcaaaagga atggggctgg aacaagccgc cctacttcga
     1861 ccgcgtcggc gaagagcatc aagccacgcg tgaacgtgtc actctctttg acctgacctc
     1921 tttcggcaaa attgaagtca ccggacccgg cgcgttgagt ctcttacaac gagtttcaga
     1981 tagcaacatg gataaaccgg taggcagcgc cacatataca caatttttga atactcgtgg
     2041 tggcgtcgaa gcggacgtga ccgtcacccg ctttgcagag gataaattct ggttgtgacc
     2101 ggatccggtt ttatcgcaaa cgatctggcg tggctacaga tgcatcatgg atcggatgac
     2161 gtgaagattc gcgacatcac gcaggattac gcatgtctcg cgttttgggg accaaaagcc
     2221 aggcacgttc tcgcaaaaat aacccaggac gatgtcacca acgaagggtt tccatacctg
     2281 tcagcgagat acattgaaat taacggagtt aatgtctggg cacagcgcgt tagctatgtc
     2341 ggtgagctgg gctgggagtt atacatccca aacaaccgcg cttcgataat ttgggacttg
     2401 ttgcgcaaag caggaaaaga atttgatatg gaagtcggcg gttataaagt tctcgattca
     2461 ttacggctcg aaaaaggcta tcggtattac acgaccgatg taacgccttt ggaaactccc
     2521 tatgaagcag gcctgggctt ttgtgtccat ttggataaag gagactttat tggcaaagat
     2581 cagctaatcg agaaaaagaa aaacggcttt tcaaccaagt tatgtacttt ggttctggat
     2641 aatgatgaat acacacagat ttatggcggt gaagctgtct accatgaagg cagagtgtta
     2701 acgcggattc gcagcggggg gtatggatat acactgaaga aaaatattct ctacgcgtat
     2761 ttaccgatgg aactctcgaa gccgggttcg cgcgtggaag tggaattgat tgagggaaga
     2821 tatattgcag aagttactgc agcggtcttg tatgacccca aaagtgaaaa actacgagcc
     2881 tgatgttcct tcaatatcct gacctttgaa gttaaaagat ataatacgtc catgcctaag
     2941 agcgctcctg actacgtcgg atttgggatg ctaacgcctg ttgagatcat ggtgttggag
     3001 aaattcccca ggctcaatac cggcgccatc gtaaaagacg tcaaccagtt tatatttgat
     3061 gatgcagcca ttgtggcatg tttgttgcgt caatgggaag tgtccgcggg aatcattggg
     3121 acggccgtag gcgatgacct gcgtggtcgt gcacttgcca agcaattgaa agaatgggga
     3181 gtacagggaa aagttcggtt tacgaacgag tataagacac ccattgaagt caatgtatct
     3241 gatcgaagag gcgcgcgcac ctacttttgg cagcggaccc ctcaaatatt gaagaccctc
     3301 gacacagcgg atttgtctct gttacgcggc gctaagatca tgtatgccga ttggtacgat
     3361 ggtgaccata ttctgcgagc catggatgaa gccaagcgat tagatgtgcc cgtatttgtc
     3421 aatttggaac atggacatag gaaagctgaa actttaaaga agtttgccga gcgcgccacc
     3481 atctgtcagg ccgtgactga cgctgcgcag ttgggtggca agcaagccct tgtgggaaca
     3541 gcaaaaaaat tattgaaatc gggtatccag acggccatca tcaccctcgc aaaaggtggc
     3601 tgtctggttg cgcaaggtga tgagatcgtc cgcgtgtttg ctccgcaggt cagggcagtg
     3661 gatgcctctg gcgcgggcgc gaccttttca gccggtttta tttatggtta tcttaaggga
     3721 tggaatcttg aggcgtctgc ccgttttgca actgccgcgt ctactttaaa agtaacacgc
     3781 gccggtttac aaatgttccc tgtcgaacag attttgggcc tggctgccgt actaaaagtg
     3841 gaacactggg cattcagaaa taatcgcttt gagatcatcg atcgaatact ttccatccca
     3901 cggcagatgg cggaccaggg caagcgcgcc caacaaaaga ttttacgcga gttgcgtttc
     3961 cccaaaaagc agccagggcc tcgaaatgtt gaaactggac aatcaaaaac cagacgaaag
     4021 actgtttagg tactacttcg tcgcgcgcac accgcaacaa aaaaaattaa ttgccgcgac
     4081 actgtgactc tgtagcagaa aatttttcag gatgtgacac atgtcatttg cagaactcct
     4141 cacacaggaa caagtcgaac gtgttcatga agcctccctg gaggttctcc aggaagtagg
     4201 cttaaaagta cgctatgcac cagcgcgtga ggtcttcgcg cagcatggtg cgaaaaccga
     4261 agccgatagt gaattcatca aacttccgcg ggacgtcgta gaaaaatatc gaaagatggc
     4321 gccgcccaag tttaccttcc gtggccgcga tcccaagttc gacaagacca ttccggacga
     4381 cagcccagtc attgtgaccg cgagctctgc ccccgatatc attgacccgg tcactggcaa
     4441 ggaaagacgt gcggaatcag gtgatattgc acgcatcgct cacctcatca atgaactgcc
     4501 cggctatgac atgttctcta tttcgaccct ggccgatgat gcacccagag atcaattcac
     4561 gatttccagg ttgtatcctg cgctcaagta ttgcctcaaa cccatacgcg tcaccaccac
     4621 tgaccacaaa gacacgcttg ccatgatgga gatggcttat attgtcgcgg gtggcaaaga
     4681 tgcttatatg gaacatccct tcctcacgca tcattactgc ccgacggttt caccgctttc
     4741 catggatgtg ctctccacgg aaaatgtgat gtacttcgcg ggcgaaggct tgccagtcta
     4801 cccaaccatc gtgcccaacg cgggactcac atcgcccatg tcgatggctg ggacgttagt
     4861 gca
//
LOCUS       DASTJP010000484         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_876438, whole genome shotgun sequence.
ACCESSION   DASTJP010000484 DASTJP010000000
VERSION     DASTJP010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876438"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..886
                     /locus_tag="VFQ23_14110"
     CDS             74..886
                     /locus_tag="VFQ23_14110"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET9907780.1"
                     /translation="MTQWRYDRLTWTEMKDVIARDPQPVVCVPFGTVEDHGTHLPIST
                     DNDILEAVLSECGRRAEGDLLITPTIPVGLDEHHMDFPGTLSVDMETCLAYVSQVAIS
                     VARHGFTHIFIVNGHGSNQSICELAARKVVLETGVICANTTINAAVNPALVADVLDAN
                     RKGGWGSVGHACEYETAMMLYSYPELVQMDKATRDVGQLKLKYFNWDFPEPSAYSWQD
                     WWSRFSKEGVCGDASAATAEFGQLMFETTVTRFIEMAREFRTIPIKARVDHH"
     gene            1048..1524
                     /locus_tag="VFQ23_14115"
     CDS             1048..1524
                     /locus_tag="VFQ23_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907781.1"
                     /translation="MSYELLNPYTTISRTFSRQFWSKALALAELYGWQPMGTEPPSEH
                     DFDEINAEWDGTYLTNDGQMIKAQDALSLAFALQNALKDIPGAKIPVALNAGFWMEAD
                     LPDWFSPDELEMVDDGLENELLDFNGTPPLEFFAGDEKEHLQQFIRFCRLGNFVIS"
     gene            1668..3089
                     /locus_tag="VFQ23_14120"
     CDS             1668..3089
                     /locus_tag="VFQ23_14120"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET9907782.1"
                     /translation="MPPSETPPPTWTPFPTEVLATFTPTVFPTLTPELERPRYVIDLQ
                     LNYSAKAAVVSQTITYPNWTGEALTNLVLAVEPNLWSGGFNLSSLSVDEQPITNYTIT
                     NSNQRLEFPLLEPLPPSGTITITMTYGLILPQMQAYSDPNEVRPQIYGYSDKQINFVD
                     WYPFVVPYIPGQGWVLHNPWFYGEHLVYDVADFDVKVTLTDGTQPQIAASGVELQQSP
                     TESGRRFVLEAGRTFALSISTDFKVAMQVVGDVTVYSYYFGFYDEAGQALLQTTVQAL
                     QVYSERFGPYRHRTLTAVQGDFNDGMEFSGLYFISRDYFNLYKGTPENYLVTIGAHET
                     AHQWWFDAVANDQGLEPWLDEALATYSEVLYYESIYPELVDWWWEYRYFEFMAPGSVD
                     TAVYDGGGQRPYWDKVYLTGARFFQDLRDRLGDDIFFAFIKDYYAQYAGRRATAVDFF
                     RVLREHTAADISDLMAQYFKNTY"
     gene            3119..>4205
                     /locus_tag="VFQ23_14125"
     CDS             3119..>4205
                     /locus_tag="VFQ23_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907783.1"
                     /translation="MNKKKIITLCVLFGFILSSCSLPVAPPAAGSGQNFVLVTEDSGA
                     LPTSTPFQPVTATIGPLESPTLIPTFTPLPPSDTPLPTLEFTATTLPSPTASPPSSRT
                     QYTMFALLDYYGRQLAVDETVKYTNQTGVALGEVVMAVEPNLSGGFTLENILLDGTPL
                     NYDLSGQRLTVYLPQPLAPAAQLTLAMRFRIAIPSKAREHPYGYDANQVNLTDWYPFI
                     VPYNNGWVLHDEWHLGEHLVYDASDFEVNARTTDPGITFAASGVGEPNGEWTRYRLTG
                     ARTFALSASDQYLMVDSAVGAAAIRAYYFPGYDYEGLAILNAAVRSVGLFESLFGPYP
                     YGSLSVVQSDLNDGQEYDGLVFLGSKFY"
BASE COUNT          978 a         1017 c         1083 g         1127 t
ORIGIN      
        1 cggagacaca gagaaaatac ttaaaatctt tgtgaacttt gtttttaaat aaggataaac
       61 cttggaggca tacatgacac aatggcgtta tgaccgtttg acctggactg aaatgaaaga
      121 cgtgatcgca cgagatcctc aaccagttgt ttgcgtcccg tttggcacag tggaggacca
      181 tggaacacac cttccgatct cgaccgacaa tgatattctc gaagctgtgt tatccgagtg
      241 cgggcggcgg gcagaaggtg acctgctcat cacgccgact attcccgtcg gtttggatga
      301 acatcacatg gacttccccg gtacgttatc ggtagacatg gaaacctgtc tcgcttatgt
      361 atcacaggtc gcaatttctg tggctcggca tgggttcacg catatcttta ttgtcaatgg
      421 gcatgggtcg aatcaatcga tctgcgagtt ggcagcgcgt aaggttgtgc ttgaaacggg
      481 agtcatttgt gcaaacacca cgatcaatgc tgcggtcaac cctgcgctgg tggcggatgt
      541 tttggatgca aatcgcaaag gcggctgggg cagcgtcggt cacgcctgcg aatatgagac
      601 ggcaatgatg ctgtatagtt atcctgaact ggttcaaatg gataaggcca cgcgcgacgt
      661 tgggcaactc aagttgaaat atttcaattg ggatttcccc gagccaagcg cctatagctg
      721 gcaggactgg tggagtcgct tctcaaagga aggggtttgt ggagatgcct ccgcggcgac
      781 ggcagagttt ggtcagttga tgttcgagac caccgtgacg cgttttattg aaatggcgcg
      841 cgagtttcgg actattccga ttaaggcgcg cgtggatcat cactaattct tgccacagag
      901 tcgccgagag aacataatcc tccgcgtcac catggcaggt tggtctattt cttcagatag
      961 ctgtccaatt taatcgtgaa ttggaatttg ctttagacgc aaaggattta taattctgca
     1021 gcttgcacag caatcatcga tacgactatg agttacgaac tacttaatcc ctataccacg
     1081 atttccagaa cgtttagccg gcagttttgg tcaaaggcgc tggctttggc tgagctttat
     1141 ggctggcagc cgatgggaac agagcctcca tctgaacatg attttgacga aataaatgct
     1201 gaatgggatg ggacgtatct gacgaacgat ggtcagatga taaaggctca ggatgcatta
     1261 tcgctcgctt ttgctttgca aaatgcactg aaggatattc ctggtgcgaa aatccccgtg
     1321 gctttgaatg caggattctg gatggaggct gatctcccgg actggttttc gccagatgaa
     1381 ctggaaatgg tcgatgatgg gctggaaaat gaactgctcg atttcaatgg aacgccgccc
     1441 ttagagtttt tcgcgggtga tgaaaaagag cacctccaac aattcatcag attttgcagg
     1501 ctggggaact ttgtgatttc gtgaatcgtc ccaataccag acggggatag cacgatatca
     1561 aatgagataa aatacgctcc atgtttcgcc ttgctatttc tcgtatagtt ctttcctctt
     1621 gcctctttct tttcatctcc tgttcttcgt taaccccttc aactgtggtg cctcctagtg
     1681 aaacgcctcc tcctacgtgg acacctttcc caacggaggt gctggccact ttcactccca
     1741 cggtctttcc gacgctcacg cctgaattgg aacgcccgcg ttatgtgatt gacttgcagc
     1801 tcaattactc agctaaagcc gcggtggtta gccagacaat cacctaccct aactggacgg
     1861 gtgaagccct tacaaatctt gtgcttgcag tagagccaaa cttatggagc ggtggattca
     1921 atctcagctc tctgtcggtc gatgagcagc caatcacaaa ttacacgatc acgaactcca
     1981 atcaaagact cgaatttcca ttgctggaac cattaccgcc aagcggaacg atcactatca
     2041 caatgaccta cggtttgatc cttccgcaaa tgcaggccta cagcgatcca aacgaagtac
     2101 gcccgcagat ctatggatat tcagacaaac agatcaattt tgtggattgg tatccgtttg
     2161 ttgtgcctta tattcccggg cagggctggg tgctgcataa cccgtggttt tatggcgaac
     2221 atcttgtcta tgatgtggct gacttcgatg taaaagtcac tctgacggat gggactcagc
     2281 cgcagatcgc tgcgtctgga gtggaactcc aacaatctcc gacggaatca ggccgccgct
     2341 ttgttcttga agcggggcga acatttgccc tatccatcag cacggatttc aaagtcgcga
     2401 tgcaggtggt tggtgatgta actgtctata gttattactt tggcttttac gatgaggcgg
     2461 gccaggctct cttgcaaacc accgtacagg cgttgcaagt gtacagtgaa cgctttggtc
     2521 cataccgtca caggacgttg acagccgttc agggtgattt caatgacggg atggaatttt
     2581 cgggtttgta tttcatcagc cgcgattatt tcaatttata taaaggtaca ccggaaaatt
     2641 atctggtgac gatcggtgcg cacgagaccg cgcatcaatg gtggttcgat gcagttgcga
     2701 acgatcaggg actcgagccc tggctcgatg aagcgctcgc cacctatagt gaagtgcttt
     2761 attacgaatc aatttatccc gagctggtgg actggtggtg ggaatatcgc tattttgaat
     2821 ttatggcgcc gggcagtgtg gatacagcag tctatgacgg tggcggtcag cgtccctatt
     2881 gggataaagt ctacttgacg ggtgcgcgct tttttcagga tttgcgtgac cgactgggcg
     2941 acgacatttt ctttgccttc atcaaagatt actatgctca atatgcgggc agacgcgcaa
     3001 cggctgttga ttttttccgt gttttgcgcg aacacactgc ggcggatatt tcagatttga
     3061 tggcgcaata ctttaaaaat acgtactgat gctcagcata ttcttatcac tgaggtcaat
     3121 gaataaaaag aaaataatta ctctctgtgt tctgttcggt tttatcctat cctcatgtag
     3181 cttaccggtg gcacctcccg cggcaggctc ggggcaaaac tttgtgctgg tcaccgaaga
     3241 tagcggcgcg ttgcctacct ccacgccgtt tcagccggtg accgcgacca taggaccgct
     3301 tgaatctcca acgctgatcc caacgtttac gccactcccg ccaagtgata cgcctcttcc
     3361 taccctcgaa ttcactgcca cgactcttcc atctcccaca gcctctcctc cctcttcccg
     3421 aacacaatat acgatgttcg ccttgcttga ttattatggt cgccagctag ccgtggatga
     3481 aacggtcaaa tataccaatc agaccggggt tgcgcttggg gaagtcgtaa tggctgttga
     3541 gccgaatctt agtggtggct ttacactcga aaacattctc ctggacggca cgcccctgaa
     3601 ctatgatcta agcggtcagc gactgacagt ttacctgcct caacctcttg ccccagctgc
     3661 tcagctaact cttgccatgc gttttcgtat tgcaatccca tcgaaagcca gagagcatcc
     3721 gtatggttat gatgcaaacc aggtcaacct gacggactgg tatccgttca tcgttccgta
     3781 taacaatggc tgggttttgc atgatgagtg gcatttgggt gagcaccttg tttatgatgc
     3841 gtcagatttt gaggtgaatg cccgaacaac ggatcctggc atcacgttcg cggcaagcgg
     3901 agtcggtgag cccaacggcg aatggacgcg ttatcgtctg actggtgcgc gtacgtttgc
     3961 cctatcagcc agtgaccagt atctgatggt ggattcagct gtcggtgcgg cggcgatcag
     4021 ggcgtattat tttccggggt acgattacga agggctggca atcctgaacg cggctgtgcg
     4081 ctcggtgggg ctgtttgaat cccttttcgg accctatcca tacggaagtt tgagcgttgt
     4141 tcaatctgac ctgaacgatg ggcaggaata tgatgggctt gtatttcttg gctcaaagtt
     4201 ttacg
//
LOCUS       DASTJP010000485         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1312742, whole genome shotgun sequence.
ACCESSION   DASTJP010000485 DASTJP010000000
VERSION     DASTJP010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312742"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2196
                     /locus_tag="VFQ23_14130"
     CDS             <1..>2196
                     /locus_tag="VFQ23_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9907784.1"
                     /translation="KGKSEPVLAYQALNRKYESGRTRGIEGLNAAMVGREEELSALRN
                     TLADLKQGLGRIVFVLGEAGLGKTRLVSEAHEVFKTLGHTQTLWAETICLSYETNQAY
                     GLFQRLIRRIGGIDYNDSPTVLHEKLSALVEDMNEAQRVRASRVFEVLFGLESGKNGV
                     PMDDETFRRDLYEAMYLWWKSRFSLQPAVLVFDDMHWCDAASAELLRQLLPLTSESPL
                     VLLFAMRAERNAPAWQIKSNADEEYNHLYSELVLHSLSEAEGNELVNRLLENPQLPIT
                     LRAGILEKSGGNPFFIEEVVRTLIDNGVLIAERQALNGRTKRVWRATQASADFAIPDN
                     LQSLLAARMDRLEESTRGTLQLASVIGRSFYHRVLQEVDEASHDLDKHLGTLLRMELI
                     REAARLPEVEYAFRNPLTQEAVYKTILIKRRRAFHGRVGEAMETIYADRLDGLFGLLA
                     HHFTLANDPVKAIDYCRKAAWQAVSLFAYDDAIQNLRSALALIEEGKQIEIHTTLLEE
                     LGDVYSLLRDIGQAIATYHDSLHLWESLPQRDEITAIRLHRKIVQLATEAKWNVDLAM
                     YQHVKDLTLESLSELELKLSILKYEPHAEVVRALATLSFEAWRSRMPPDWERAQKFAQ
                     SAVDMAEELNEPVVLSRALGALCSVLDGRSLLSEHLQFALRRLEVSQDPRVDNPIELI
                     DAISSAGMALMYVGEYEQAIPHLQEAEKLASRAQAIGQQTAAIGLQSQCA"
BASE COUNT          518 a          536 c          632 g          510 t
ORIGIN      
        1 gaaggggaag agtgagcccg tactggcgta tcaggctttg aatcggaaat acgaatcggg
       61 ccgcacacga ggcatcgaag ggctgaacgc ggccatggtg ggccgcgagg aagaattatc
      121 agccctgcgc aatacgttgg cagatctcaa acagggtctg gggcgtatcg tattcgtatt
      181 gggtgaggca ggtctgggca agacccgcct ggtcagcgag gctcatgagg tatttaaaac
      241 gctcggacat acccagaccc tctgggctga aacgatctgc ctttcttatg aaaccaatca
      301 ggcgtatggg ctattccaac gtctcatccg ccgtatcggg ggcattgact acaacgattc
      361 accgaccgtt cttcatgaaa aattaagcgc cctggttgaa gatatgaatg aagcgcaacg
      421 agtacgcgcc tcacgagttt tcgaagtctt gtttggattg gaaagcggga aaaatggcgt
      481 accgatggac gatgaaacgt tcagacgtga cctgtacgag gccatgtacc tgtggtggaa
      541 atcgcgcttc tcactccagc ccgccgtatt ggtgttcgat gatatgcatt ggtgtgacgc
      601 tgcttcggct gagttgcttc gccaattgct gccactgacg agtgagagcc cgcttgtgct
      661 tttgttcgct atgagggcag aacggaacgc gccagcctgg caaattaagt cgaatgcgga
      721 tgaagaatac aatcacctct attcagaact tgttttgcac tcgctttcag aggcggaggg
      781 caacgagttg gtcaaccgtc tgcttgaaaa ccctcagctt cctattactc tgcgggcggg
      841 cattctcgaa aagtctggtg ggaatccatt cttcattgaa gaagtcgtgc gcacgttaat
      901 cgacaacggg gtcctgatcg ctgaacgaca ggccttgaac ggccggacga aacgagtctg
      961 gcgtgccacg caggccagtg cagattttgc cattccggat aatttgcaat cgctgcttgc
     1021 ggcgcggatg gaccggctcg aagaatccac gcgcggcacg ctgcaactgg cctccgtcat
     1081 tggacgatcc ttttatcatc gcgtcttaca ggaagtagat gaagccagcc acgacctcga
     1141 taagcatctt ggcacgctgc tgcggatgga gttgattcgc gaggcggctc gcctgccaga
     1201 ggtcgaatat gcctttcgta accctcttac ccaggaggct gtttacaaaa ccattttgat
     1261 caaacgcagg cgcgcatttc atggccgcgt cggtgaagcg atggaaacca tttatgcaga
     1321 ccgtctggat ggacttttcg gtttacttgc ccatcacttt actctggcca atgatccggt
     1381 gaaagcgatt gattattgtc gcaaagccgc ctggcaggcg gtctcgctct ttgcgtatga
     1441 tgatgcgatt caaaacctgc gcagcgcact ggcactcatt gaagaaggca agcagattga
     1501 gattcacaca acgttgctgg aagaattggg agatgtgtac agtctgcttc gagatatcgg
     1561 gcaggcgatt gctacctatc atgactcact tcatctctgg gaaagtctgc cgcagcggga
     1621 tgagatcacc gcgatacggc tgcatcgcaa aatcgtacag ctcgccacgg aagccaagtg
     1681 gaacgtagat ctggccatgt atcaacatgt gaaagacctc acgttagaat ccctatctga
     1741 actggagttg aagctctcga ttttgaagta tgagccgcat gctgaagttg tccgtgcgct
     1801 ggctactctt tcgtttgaag cctggcggag tcgtatgcct cccgattggg agagagctca
     1861 aaagtttgcg caaagcgcag tggatatggc agaggaactg aatgaacccg tcgttttgtc
     1921 acgtgcgctg ggcgcgctgt gcagtgtgtt ggatgggcgt agtttactga gcgaacattt
     1981 gcagttcgca ttgaggcggc tggaagtgag ccaggaccct cgtgtggata accccattga
     2041 actcattgat gcaatcagca gcgcagggat ggcgcttatg tatgtaggtg aatacgagca
     2101 agctattcca catttgcagg aagcggaaaa attagccagc cgcgcgcagg cgattggtca
     2161 gcaaacggcc gcgattgggc tgcaaagtca atgcgc
//
LOCUS       DASTJP010000486         5977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_747085, whole genome shotgun sequence.
ACCESSION   DASTJP010000486 DASTJP010000000
VERSION     DASTJP010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747085"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFQ23_14135"
     CDS             complement(<1..422)
                     /locus_tag="VFQ23_14135"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9907785.1"
                     /translation="MKIAVTGAFSFTGKYIARRLLDRGEEVITLTGHPNRPDAFGGKV
                     KAYPLDFDEAAMARSLQGVEVLVNTYWVRFDKGENTQPRAVENTRKLINAAKTAGVKR
                     VVHISIANPSADSHLPYYWGKAANEKTVMESGMTYAILR"
     gene            complement(541..1104)
                     /locus_tag="VFQ23_14140"
     CDS             complement(541..1104)
                     /locus_tag="VFQ23_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907786.1"
                     /translation="MKSFLLALFLLCLVLLNACTLPLTPTSLPPTVTATSTQTATATP
                     PTYGGCGYQWANQPLPELSAEFQNAIQLLQPEAQANAFAFGEDCVHADGTTRTFIAME
                     TDFNVTLQVNDLSDESALGDWIVKIMDVIENIPPEKIMGPRPGRVSVAFQVGSEQKYV
                     NFYVDQYQALPSGLSSTEIYQALQTPQ"
     gene            complement(1168..2367)
                     /locus_tag="VFQ23_14145"
     CDS             complement(1168..2367)
                     /locus_tag="VFQ23_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9907787.1"
                     /translation="MPNFLEQAEELFPYTQSLRRDFHMHPELGFREVRTGGIVAKELE
                     ALGIEVTKGVGKTGVVGLLEGAKPGPTLLLRFDMDALPITEDTGAEYSSQNPGVMHAC
                     GHDGHTAIGLTVAKMLHANRDELAGNIKFCFQPSEEGVNGEEMGGNLMMIRDGVLDGP
                     TVDKTLSLHLWNDKPLGWINVAKGPVMAGAEMFFIKLTGKGGHGAAPHTTIDPIVAAS
                     QIVSALQSITSRNVPPLKSSVVSVTSFHAGTAFNVIPQTAELGGTIRTFDLAVRKTVL
                     ERFQQIVRGIAESMGCEVEITLKQVTPTVMNNDRVASSVLNTAQTIFPQTEIDTESYL
                     TMGAEDMGYMQEKVEGCYFFVGSANEEKNLIYNHHHPKFDFDEKALISGAALMATAAM
                     DILNSSS"
     gene            complement(2391..3434)
                     /locus_tag="VFQ23_14150"
     CDS             complement(2391..3434)
                     /locus_tag="VFQ23_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9907788.1"
                     /translation="MSGVNLRFINTLILSLLLVLTLTGVYGLFFPFPSVLFEIHRITG
                     WSLILLIPWKAMISLRSLGRGVDKRLDRNVMIVISVVASIATIIIIIFGLLWKWNLGE
                     YYLWIAGYGYSAIGWHWGIALYLLVPLFVIHAWRRWPHPKKSDFTGRRQMLKLLGLGA
                     ASLATWRISEVVAKNLQDKDVPRRFTGSREEGSFAGNDHPVTSGPDQGKIKLDPSTWN
                     LKLTGEVKTALTLNYADLLALSTSEVTAVLDCTGGWYTTQNWRGVRLTDLLKSAGLQE
                     PAAAVVLKGVSEYTANFTLEQAQEILLATHVGDEVLNHVHGYPLRAVVPSRRGWHWVK
                     WLTEIEVVTLSNR"
     gene            complement(3517..4245)
                     /locus_tag="VFQ23_14155"
     CDS             complement(3517..4245)
                     /locus_tag="VFQ23_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET9907789.1"
                     /translation="MIEQTDQWENSQKILVILAHPDDPEFFCGGTLARWARAGHEITY
                     VLLTCGDKGFNPATQADMTPEKLCEIRYEEQQNAAKIIGAKSIHFLGKADGYLTPDLN
                     LRRDVVRVIRQHKPDILVTCDPQTLFAIYGINHPDHRAAGQVVLDAVFPAAGNLTFFP
                     ELIEEGYPPHMPKEVWCSLTMQPNTAIDVTDMWDIKLNAILEHKSQVQDVEKLLERLR
                     SRYTEDSTIENPRYEEKFRVVKYS"
     gene            complement(4229..5161)
                     /gene="ricT"
                     /locus_tag="VFQ23_14160"
     CDS             complement(4229..5161)
                     /gene="ricT"
                     /locus_tag="VFQ23_14160"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HET9907790.1"
                     /translation="MQPNIIGVRFTKVGKIYHFDSTAVPDLGIGEHVIVDTSRGRHLG
                     EVVQVVKETPPKPEGGWRSVERRATPRDLLLLQSWQSKQTEAMINCRARAAELRLKEI
                     KIVAAEYNYDGSRLAFLFSTESEEKVDLKSLKKDMHDLYPNTHIEMRQIGPRDVAKIL
                     GGMGACGLETRCCSKFLTDFSPISIKMAKDQGISLTPNEITGMCGRLRCCLIYEYEQY
                     VEARKQLPKRNKRVITPKGEGKVIDVLPMNNKVLVLIEGEEGKRFTETFDREDLQPWD
                     ELEALRRKSEAPCDRHEGGGCTCGKARKRNDRAN"
     gene            5364..>5977
                     /locus_tag="VFQ23_14165"
     CDS             5364..>5977
                     /locus_tag="VFQ23_14165"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9907791.1"
                     /translation="MNPKRPFLYALTAIVTILTLVLSGCGATPAPATEVPPGELTGTL
                     SVLDWAGYDAEDFWIDFKNTYPNVTVNFEIGISDADIYAKMAAGNQADVFHPYTGWLQ
                     FYVDEGLVEEIDTSKLTNWDKVPDHFKQIGQINGKQYFIPWDWGFTSVLYRTDMIPDG
                     VDSWDALMDPALAGHISMWDDGPGAVTISSYIHGYDETNITAEQL"
BASE COUNT         1441 a         1460 c         1507 g         1569 t
ORIGIN      
        1 cgtaggatag catacgtcat acctgattcc atcacagttt tctcattagc tgccttaccc
       61 caatagtaag ggaggtgtga atcggcagat ggatttgcaa tgctgatatg cacaactctt
      121 tttacgcccg cagttttcgc agcgttaatc agtttacggg tgttttccac tgcacgtggt
      181 tgagtgttct cacccttatc aaaacggacc cagtaggtat tgaccagcac ctctacgcct
      241 tgcagactgc gtgccatggc cgcttcgtcg aaatcgagcg ggtaggcttt gacctttcca
      301 ccaaatgcgt cagggcgatt tggatgtccg gtcaaagtaa tgacctcctc gccgcggtcg
      361 agcaatcttc ttgcgatgta ttttccagta aaactaaaag cacctgtaac agcaatcttc
      421 attttggctc catcttgtga aatatatcac aattgaatta taagaatatc cacctgattt
      481 gtcaagattc aaaaaaagcc gctactcgtt gaagtggcgg cttaaaatcg tggatgaagt
      541 ttattgcgga gtttgaaggg cttgataaat ttctgtgcta ctgaggcctg aggggagggc
      601 ctggtactga tcaacataaa aattcacata cttttgttcg cttccaactt gaaaagcaac
      661 acttactcta cccggccgtg ggcccataat tttctcgggt ggtatatttt ctatcacatc
      721 catgatcttt acaatccaat cgccgagcgc cgattcatct gacaggtcat tgacctggag
      781 cgtaacgttg aagtccgttt ccatcgcgat gaatgttctg gttgttccgt cggcatggac
      841 acagtcttcg ccgaaagcaa aagcattcgc ttgtgcctcg ggctgtaaca gttgaatggc
      901 attctgaaat tccgcagaaa gttcgggcaa gggctgattt gcccattgat atccacagcc
      961 gccgtaggtg ggcggcgtgg cagtggcggt ttgggtggat gttgccgtca cggtgggcgg
     1021 caatgaagtt ggcgttaggg gtaaggtaca cgcatttagc aaaaccaggc agagaagaaa
     1081 aagggcgaga aggaagcttt tcatatatga aaccaacgat tccttctctg cctttgttcc
     1141 caccattcat tcaacagggt agctgtttca tgaactggaa ttcaaaatat ccatagctgc
     1201 ggtcgccata agtgcagcgc cgctgatcaa tgctttttcg tcaaagtcaa attttggatg
     1261 gtgatggttg taaatgagat tcttttcctc atttgccgag ccgacgaaga aataacagcc
     1321 ttctactttt tcctgcatat atcccatatc ttctgcaccc attgtaagat aggattcggt
     1381 atcaatttct gtttgaggga agatggtttg agcggtgttc aagacgcttg aagcgacacg
     1441 atcattattc ataaccgtgg gcgtcacttg tttcagagtg atttccacct cgcaccccat
     1501 cgactcagcg atgccgcgca caatttgctg aaagcgttcc aggacagttt tgcgtaccgc
     1561 gagatcgaat gtgcgtattg tgccgccaag ttcggctgtc tgcggaatga cgttgaaggc
     1621 cgtgccagca tgaaaagatg tcacgctgac gaccgatgat ttcaagggtg gaacgttgcg
     1681 tgatgtgatc gactgtaacg ccgaaacgat ctgagaggca gcgacaatag ggtcaattgt
     1741 tgtatgaggt gccgcgccat gcccgccttt cccggttagc tttatgaaga acatttctgc
     1801 gcccgccatc accgggcctt ttgcgacgtt gatccagccg aggggtttgt cgttccacaa
     1861 atgcagtgac agagtcttat ccaccgtagg accatctaaa acaccgtcgc gtatcatcat
     1921 cagattgcca cccatttctt ccccgttgac gccctcttca gatggttgaa agcagaactt
     1981 gatattgcct gccagttcgt cgcggtttgc gtggagcatt ttcgccacag taaggccgat
     2041 tgccgtatgg ccgtcgtgtc cgcaggcgtg catcaccccg ggattttggg atgaatactc
     2101 cgcgcccgta tcttccgtga tggggagggc gtccatgtcg aagcggagga ggagagtcgg
     2161 tccgggtttc gcgccttcca gcaaaccaac aacgcccgtt ttcccgacac ctttcgttac
     2221 ttcgatccca agcgcttcca attctttcgc gacgattcca cccgtgcgga cctcccggaa
     2281 gccaagttct gggtgcatgt gaaagtcgcg acgcagggat tgagtgtatg gaaataattc
     2341 ttcggcttgt tcgagaaagt taggcatgtg tgggttcctt tagaatgtaa ttatcgattt
     2401 gaaagggtga caacttcgat ttctgtcagc cattttaccc aatgccagcc gcggcgtgag
     2461 gggacgaccg cgcgcaaagg atagccgtgc acatgattta gcacctcatc accgacgtgg
     2521 gtagctaaca aaatttcctg cgcctgctcc agagtaaagt tcgctgtgta ttcagaaaca
     2581 cctttcaaca caaccgcggc tgccggttct tgcagcccag cgcttttcaa tagatcggta
     2641 aggcgaacgc ctcgccaatt ctgagtagtg taccagccgc cggtacagtc caaaacagca
     2701 gtaacttcag aggtagacaa agcaagcagg tctgcataat tcaacgtcag cgccgttttc
     2761 acttcgccgg ttagttttag attccacgtt gatggatcca gcttgatttt gccctgatcc
     2821 ggtccactcg tgaccggatg atcattccca gcaaaggaac cttcttcgcg ggaaccagtg
     2881 aaacgacgcg gtacatcttt gtcttgaagg tttttggcta ctacttctga gatacgccat
     2941 gtcgccaagg atgccgcacc caatccaaga agcttaagca tctgccgacg acccgtgaaa
     3001 tccgatttct ttggatgcgg ccagcgacgc caggcatgga tgacaaagag tggcactagt
     3061 aaatacagcg caatgcccca gtgccagccg atagcgctgt atccatatcc cgcgatccag
     3121 agataatatt cgccgagatt ccatttccaa agtaagccaa aaataataat gatgattgtt
     3181 gcaatggagg ctacaaccga gatgacgatc atcacatttc gatcgagacg tttgtccacg
     3241 ccgcgtccca acgagcggag tgagatcatc gccttccagg ggataagaag tattaatgac
     3301 caacccgtga tgcgatggat ttcaaataag acagatggga acgggaaaaa cagaccatag
     3361 acgcctgtga gagtgagaac cagcaacagg ctaaggatca atgtgttgat gaagcgtaga
     3421 ttcacaccgc tcatcaaggt ctcctctgaa ccgcaaagta cgccaagccc acgaagattt
     3481 agtttataaa cgctttgagt acgttccggt taataatcaa ctgtatttca ccacgcggaa
     3541 tttttcttcg tagcgtggat tctctatggt gctgtcttcg gtatagcgag atcgaagacg
     3601 ctccagcagt ttctccacat cctgcacctg ggacttgtgt tcgaggatgg cattgagctt
     3661 gatatcccac atgtcggtaa catcaatggc cgtattgggc tgcatcgtta gggaacacca
     3721 cacctctttg ggcatgtgcg gcggatatcc ttcttcgatc agttccggga agaatgtgag
     3781 attccctgcc gctggaaaaa ccgcgtcgag taccacttga ccggcagcgc ggtggtccgg
     3841 atgattgatg ccgtatatgg cgaataacgt ctgagggtcg caagtgacaa ggatatctgg
     3901 tttgtgctgg cggatcacgc ggaccacgtc tcggcgtagg ttaagatctg gagtcaggta
     3961 accgtctgcc ttgcctagaa aatgaattga tttagcacca atgattttgg ctgcattttg
     4021 ctgctcttca taacgtatct cgcataactt ttcaggagtc atatctgcct gcgtggcagg
     4081 attaaagcct ttgtcaccgc aggttagcag tacatacgtg atctcatgtc ctgcgcgagc
     4141 ccaacgggca agtgttcctc cacaaaaaaa ttctgggtca tcaggatggg ccagaattac
     4201 cagaattttt tgagagtttt cccactgatc agtttgctcg atcatttcgc tttcttgctt
     4261 tgccacaggt gcagccgccg ccttcgtgac ggtcacaggg cgcttcggat tttcgccgca
     4321 gcgcttcgag ctcgtcccag ggttggaggt cttcacgatc aaatgtttca gtaaaccgtt
     4381 tgccttcctc gccttcgatc agcaccagta ctttattgtt catggggagg acgtcaatca
     4441 ccttgccctc gccctttggc gtgatcacgc gtttattgcg cttggggagc tgtttgcgtg
     4501 cctcaacata ttgttcgtat tcataaatca gacaacagcg aaggcgccca cacatgcccg
     4561 tgatctcatt aggcgtaagt gaaatgccct gatcttttgc catcttgatc gaaatggggg
     4621 agaagtcggt gagaaatttg gaacagcagc gggtttcaag tccgcatgcg cccatgccgc
     4681 cgaggatctt tgctacatcg cgcgggccga tttgtctcat ttcaatatgc gtattgggat
     4741 aaagatcatg catgtccttt ttgagggact taagatcaac cttttcttca ctttccgtgc
     4801 tgaacaggaa ggcgagccgc gatccatcgt aattgtattc tgcagctaca atcttgattt
     4861 ccttaagacg aagctcagcc gcgcgggcgc ggcagttgat catggcttct gtctgtttgg
     4921 attgccagga ttgaagcaaa agcagatcgc gcggggtagc gcgtctctcc acggacctcc
     4981 agccaccttc cggttttgga ggcgtttcct tcaccacctg aacgacctca cccaggtgtc
     5041 tgccgcggga tgtatccaca attacgtgct cgccgatgcc caggtcaggg acggcggtag
     5101 agtcgaaatg gtaaatcttg ccaactttgg taaatcggac accgatgata tttggttgca
     5161 taacgagaat tataccgtat ggatgaatag cacggaaata agggatttcg caattgcaac
     5221 ggtgctataa tcatttgaat tattcattga aaattgatga ggttggtacg ccaggaggga
     5281 ggtgctttcg gcttaagtag tgcgctgttc gatttggttg gagaaagatt ctttggtact
     5341 ttataaaaac gaggagaagg aaaatgaatc ccaaacgacc ctttctgtac gctttgactg
     5401 caatagttac tatactgacc ttggtgctct ctggctgtgg agccacacct gcacctgcca
     5461 ctgaagttcc tcccggggaa ttgacaggta cactttccgt cctcgattgg gccggatatg
     5521 acgccgagga cttctggatt gactttaaga acacctaccc aaatgtaacc gtaaactttg
     5581 aaattggcat ctcagacgct gacatctacg ccaaaatggc agcgggcaat caggcagatg
     5641 tcttccatcc ctatactggt tggctgcagt tttacgtcga tgaaggtctg gtggaggaaa
     5701 tcgatactag caaattgacg aactgggata aggtcccgga ccacttcaag cagatcggtc
     5761 aaattaacgg caaacaatat ttcatcccct gggattgggg ctttacgtct gttctgtatc
     5821 gcaccgatat gattccagat ggtgttgatt catgggacgc gttaatggat ccggcactgg
     5881 caggacatat ctccatgtgg gatgacggcc ctggtgcagt gacaatttcc tcctatattc
     5941 atggatatga tgaaaccaat atcaccgcag aacagct
//
LOCUS       DASTJP010000487         6267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_992340, whole genome shotgun sequence.
ACCESSION   DASTJP010000487 DASTJP010000000
VERSION     DASTJP010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992340"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFQ23_14170"
     CDS             complement(<1..340)
                     /locus_tag="VFQ23_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907792.1"
                     /translation="MNPIFQHPRYLLKRQAIALTGKFRFYDPNGTQVMFSEQKMFRLR
                     EDIRVYSDESKTQEVLSIKARQIMDFSASYDVVDTALNQTVGVLRRRGLRSLLRDEWE
                     ILDANDRMVGL"
     gene            complement(411..1442)
                     /locus_tag="VFQ23_14175"
     CDS             complement(411..1442)
                     /locus_tag="VFQ23_14175"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9907793.1"
                     /translation="MKLNPQLTRRDFLKLVSAGTLAFALKDLRIDRALAQSTIKYGRI
                     TWSGIPLYDAPNFIANKIHHFGADQVLDITGVEENGEQGNPFNSAWYLVNGGYVYSGW
                     IQPVEKNYQKPIFSIPEKGQVGEISVPFSITRKAPYVYADNGYRLYYGSTHWVQRVIV
                     QREEKSIWYEIYDYYLKKHFYVSSHDMRLIPNDELSTLSPEVPDEEKHIVVDLSTQMV
                     TAFEGEKLVFSERCASGVQGTDTPKGEFRTYHKGPSVHMTNEGDAVENESVYSLPGVP
                     WCAFFTGAGNAFHGTYWHNDYGRPRSHGCVNLPSESAKFLYRWSKPDVPPDEDYVHLP
                     GQGTRIQIF"
     gene            complement(1553..2575)
                     /locus_tag="VFQ23_14180"
     CDS             complement(1553..2575)
                     /locus_tag="VFQ23_14180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9907794.1"
                     /translation="MSRSSLSRRDFLKLSTYGLMGMYLPDLSPYLNELENLQARIIDR
                     ILWAYDEPSPKAKRTKVYWHDLILPITNTTISDDEAAYNRVWYQVDDGGYVYSGSIQP
                     VRTILNQPQQISLRGALGEITVPFTEARLEPDANAEVVYRLYFETVHWAAASAIHPVD
                     NSVWYALLDDKFDKYYYIPAEHLRLIPDSELTPLSSHIPNENKRIEVRLDEQLLLAYE
                     NRQLVFATRTSTGGKRLSGKYITPVGEFITYHKRPTRHMANGDIASNGFDLPGVPWVL
                     YITESGISFHGTYWHNDYGRPRSHGCINLTPQAAKWLYRWTMPTVAPDKYLVYGYVGT
                     KVEIKV"
     gene            complement(2627..3811)
                     /locus_tag="VFQ23_14185"
     CDS             complement(2627..3811)
                     /locus_tag="VFQ23_14185"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9907795.1"
                     /translation="MNTKFIEARENVIKARDALRRGDKKSARQLGEQAALLAPEMEDV
                     WLVLAASDPNLNEALAYARKALQINPHSTRARQGIEWVSKQLKQSETPNAPVVNLSSS
                     DAAGSKTQPKRVYREAIAAPVLRRTGPSWLLPALLLGAGFVIFALAALFVLTRPSTAS
                     LINTLGVPAFAQDNNWASVEVAKPTVTPIAVNALPVAQVADTPTSVPTSVPTKKTPLS
                     PTDAPTSAPTDAVTATAESSPTPAATETPGTMAMEIIADTPTSEAPSTISGGPYPEKG
                     NGIRWIDVNLSEQRVYAYEGDIVVNSFIVSTGTARTPTVTGTFKIWIKLKSTTMSGPG
                     YYLTNVPYTMYFFKGYGLHGTYWHNNFGTPMSHGCVNLSIPDAQWLYNWAFEGTEVNV
                     HY"
     gene            complement(3920..4627)
                     /locus_tag="VFQ23_14190"
     CDS             complement(3920..4627)
                     /locus_tag="VFQ23_14190"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9907796.1"
                     /translation="MPANRHNRFPLLLTLCLLLCGGLVTFAITTSPVRASIFSTVTPT
                     QENLWGYANIPKPTYTPISSPLGTNIPVSVPTTVPVAAEATSAMMMEIVSGSQASAGG
                     QAPVIAYNGSKYILVDISEQHMYVYESDALVYSFVASTGIGNSTRVGSFQVQTKLPNA
                     YGSTWNLWMPNWLGIYYSHGLENGIHALPILPNGATLWEGFLGSPVSYGCVVLGTYES
                     RLLYDWADMGTPVEIIW"
     gene            complement(4633..5418)
                     /locus_tag="VFQ23_14195"
     CDS             complement(4633..5418)
                     /locus_tag="VFQ23_14195"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET9907797.1"
                     /translation="MESTPSSPVRRSRPLRAVQSTLAIAILLATLFTALPSRGLVAGG
                     LYDRLGLILTPQSVENAQVSSQPQLRVGIVAGHSGNDSGAVCVDGNGNVTLTEADVNL
                     EIAALVQQQLTEIGYQVDLLREFDTRLNGYRALAIVSIHNDSCEYVNDEATGFKVSAA
                     LNTYDVNRANRLTACLVDRYQSATRLNFHAGSITPDMREYHAFREIDPSTVAAIIETG
                     FLNLDREILTQQTDRVAAGVVAGILCFSNNENVEPTAIPNLIP"
     gene            5551..6051
                     /locus_tag="VFQ23_14200"
     CDS             5551..6051
                     /locus_tag="VFQ23_14200"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET9907798.1"
                     /translation="MTLDSETLRRAMRAWSTGVAVVTAKYEDEQYGMTVNSFNSVSLE
                     PPVVSIALKQLTHTHTLVLKSGLFAVTILSAEQKELSDRFAGKLPGVTDRFEEVQIGR
                     LSIDAPIIKNGLAFFNCRVMSSTPIGENTLFIAEVIDARGEGEGNPLVYHNRMFWKLT
                     QPSPKP"
BASE COUNT         1545 a         1547 c         1608 g         1567 t
ORIGIN      
        1 acaaacccac catgcgatca ttggcatcca ggatttccca ttcatctcgc aacagggaac
       61 gcaaacccct ccgccgcaat acaccaaccg tttgatttaa cgccgtatct actacatcat
      121 acgaagcaga gaaatccatg atctgccgcg ctttgatgct caacacctct tgtgttttgg
      181 actcatcaga gtacacgcga atatcctcac gtagacggaa catcttttgt tcgctgaaca
      241 tcacctgagt tccattgggg tcataaaaac ggaatttgcc ggtcagcgcg atggcctgac
      301 gtttaagcag gtaacgcgga tgttgaaaaa tggggttcat agcatactcc ttatgaacgt
      361 agtggcgact tcagtcgcct ttgaatgact gaagtcatta ctacgctggg tcaaaagatt
      421 tgaatccttg ttccctggcc cggtaaatgg acataatcct catcgggagg gacatctggc
      481 ttggaccagc gataaaggaa ctttgccgac tctgaaggga gattcacaca accatggctg
      541 cgcggccgcc cataatcgtt atgccagtag gttccatgga aagcgttgcc tgcgccagta
      601 aagaatgcac accacggcac gccgggcaga ctatacacgg actcgttttc aacggcgtcg
      661 ccctcatttg tcatatgtac agaaggtcct ttatgatacg tacgaaactc acccttcgga
      721 gtatcagtcc cctgcacgcc gctggcacag cgttccgaaa atacaagttt ctctccttca
      781 aaggccgtga ccatctgcgt gctgagatct acgacgatgt gtttttcttc atctggcacc
      841 tcaggtgaca acgtagatag ttcatcattg gggatcaggc gcatgtcgtg cgatgagaca
      901 tagaaatgtt ttttgagata ataatcatag atctcgtacc agatactctt ctcttctcgt
      961 tgcacaatca cccgttgcac ccaatgtgtg ctgccataat acaggcggta gccattatcc
     1021 gcatatacat agggtgcctt ccgtgtaatg ctgaacggga cgctgatctc acccacctga
     1081 cctttttcag ggatgctaaa gattggtttt tgataatttt tctcgaccgg ctggatccag
     1141 cctgaataga cgtagccccc attgaccaaa taccatgcgc tgttaaaggg attgccctgt
     1201 tcgccgtttt cttccacacc ggtaatatca agcacctgat cggcgccaaa atgatggatt
     1261 ttgttggcaa tgaaatttgg cgcatcatag agtggaatgc cgctccaggt gatacgacca
     1321 tatttgatcg tcgattgggc aagagcgcgg tcgatgcgca gatcttttag tgcaaaggca
     1381 agcgtccctg cactgacaag cttgagaaag tcacgacggg taagttgagg gttgagcttc
     1441 atttcgagaa atttccaggg attagattct ctgtttttta cataaaactg caaataacag
     1501 atactaagtt taaccgcaaa gggaaggaat atgttccatt attacctttt tcttatactt
     1561 taatttccac ctttgtcccc acgtaaccat acactaaata cttatccggt gcgacggtgg
     1621 gcatcgtcca gcggtagagc cattttgcag cctgaggcgt caggttgata cagccgtggc
     1681 tgcgcgggcg gccatagtcg ttatgccaat aggtgccgtg gaaggagatt ccactctcgg
     1741 tgatgtacag cacccagggt acgcccggca ggtcaaagcc gttcgaggcg atgtcgccat
     1801 tcgccatatg ccgcgtggga cgtttgtgat atgtgataaa ttctcccacc ggcgttatgt
     1861 attttccgct cagccgtttc cctccggtag aagtacgcgt ggcaaagaca agttgacggt
     1921 tctcgtacgc cagcaggagc tgttcatcca ggcggacttc gatacgtttg ttttcgttgg
     1981 gaatatgaga agataacgga gtcagttctg aatcggggat gagacgaagg tgttcggcgg
     2041 ggatatagta atatttgtca aatttatcgt cgagaagcgc ataccatacg ctgttatcca
     2101 caggatggat ggcgctggcg gcagcccaat ggacggtttc aaaatagagg cgatacacaa
     2161 cttccgcgtt ggcatccggt tcaagccgcg cttcggtaaa cggtaccgtg atctctccca
     2221 gcgcgcctct caatgagatt tgctgaggct ggttcagaat cgtgcgaaca ggttggatgc
     2281 tgccggaata aacatagcca ccatcatcca cctgatacca tacccggttg taggcggctt
     2341 catcgtcact gatggttgtg ttcgtaatgg gaagaataag atcatgccag tacactttgg
     2401 tgcgtttggc tttcgggctg ggttcatcat aggcccagag gatgcgatca atgatgcgtg
     2461 cctgtaagtt ctctaattca ttcaggtaag gcgaaaggtc aggcagatac atccccatca
     2521 agccatacgt gcttagctta agaaaatcac gccgcgataa actcgacctg ctcattactc
     2581 atcacttccc tctcccaatg ggagagggct agggtgaggg tcattgctaa taatgtacgt
     2641 tcacttctgt gccttcgaag gcccagttgt acagccattg cgcatcggga atcgacaggt
     2701 tcacacagcc atgactcatc ggtgtgccaa aattgttatg ccagtaggtg ccatgcaagc
     2761 catacccttt aaagaaatac atggtgtaag gcacgttggt caaataatat ccgggaccag
     2821 acatggtggt ggatttgagt ttgatccaaa tcttgaacgt gccagtgact gtcggcgtgc
     2881 gcgctgtgcc agtggagaca atgaacgagt tgacaacaat atcaccttca taggcataga
     2941 cacgctgctc tgaaagattc acatcgatcc agcgtatgcc gttgcctttt tcaggatacg
     3001 gtcctcctga gatggtcgaa ggcgcttcgc tggttggcgt atccgcgata atttccatgg
     3061 ccatggtgcc gggtgtctca gtcgcagcag gggttggcga agattcagct gtcgcagtca
     3121 ctgcatcggt gggtgctgaa gtgggcgcat ccgttggaga taatggggtc tttttggtcg
     3181 gaacagatgt tgggactgaa gttggagtat ccgctacctg tgcaacaggg agagcattga
     3241 ctgcaatcgg cgtcactgtg ggcttggcga cctctaccga tgcccaatta ttgtcctgtg
     3301 cgaaggcagg cacaccgagg gtattgatca gtgatgcggt tgagggccgg gtgagaacga
     3361 ataatgccgc gagagcaaag atgacaaaac cagctccaag taataaagcg ggaagcagcc
     3421 aacttggacc cgtgcgccgc aaaactggcg cggctattgc ttcgcggtat acacgttttg
     3481 gttgagtctt gctgcctgcg gcgtcactac tactcagatt caccactggt gcattcggag
     3541 tctcagactg tttcaattgt ttcgataccc actcgattcc ctggcgcgcc cgcgtgctat
     3601 gagggttgat ttgcagtgcc tttcgggcat atgcaagcgc ttcattgaga tttggatcag
     3661 atgcagccag caccagccat acatcctcca tctcaggcgc cagcaaggca gcctgctctc
     3721 ccagttgcct ggcagatttt ttgtcgccgc ggcgcagggc atcgcgggct ttgatgacgt
     3781 tctcacgagc ttcgatgaat ttagtgttca taaatgcttc ccgtcatggc gagccaggta
     3841 tttagcaaag caatctttag ataagtttga gattgcttcg tcgccgcttt gcggcttctc
     3901 gcaacgacat ttgtattttc taccatataa tttctacagg tgtgcccatg tctgcccaat
     3961 catagagcaa ccgcgattca taggttccca gcacgacaca gccataagaa acaggactgc
     4021 ccagaaaccc ttcccaaagc gttgctccgt taggcaaaat gggcagagca tggatgccat
     4081 tttcaagtcc atgtgaataa taaatgccca gccagtttgg catccaaaga ttccaggtag
     4141 agccataggc attgggaagt tttgtctgta cctggaagga tcccacgcgc gtggagtttc
     4201 cgatccctgt ggaggcgaca aaactataaa ccagcgcgtc gctttcataa acgtacatat
     4261 gctgttctga tatatccacg aggatatact ttgaaccatt ataggcaatt acaggagcct
     4321 ggcctccagc agaggcttgt gaaccgctga cgatttccat catcatggca ctcgttgctt
     4381 cggcagctac gggaaccgtt gttggaactg aaactggaat attggtcccc aatggggatg
     4441 atatcggggt ataggttggc tttggaatat ttgcatagcc ccagagattt tcctgggttg
     4501 gagttacagt actaaatatg gaagctctga caggcgaggt ggtaattgca aaagtaacga
     4561 gtcccccgca gagcagcaga cacagggtga gaagaagtgg gaagcgattg tgtctgttgg
     4621 ctggcatggc gtttatggga tgaggttagg gatggcagta ggttcgacgt tctcgttgtt
     4681 ggaaaaacac aaaattccag ccacgactcc ggctgcaacg cggtccgtct gctgcgtcag
     4741 gatctcgcgg tcgaggttca ggaacccggt ttcgatgatc gcggcaaccg tgctggggtc
     4801 gatttcgcga aatgcatgat attcgcgcat gtctggtgtg atacttccgg cgtggaagtt
     4861 caggcgggtt gcgctttgat aacggtcgac cagacaggcc gtgagacgat tggcgcgatt
     4921 gacatcatag gtgttcaacg ccgcggagac tttaaagcca gtagcctcgt cattgacgta
     4981 ttcacaagag tcattatgaa tggagacaat tgccagcgcg cgatatccat ttagccgggt
     5041 atcgaactcg cgcaatagat ccacctgata accaatttca gttaattgct gttgaaccaa
     5101 tgcagcaatt tcaagattta catctgcttc tgtaagtgtg acatttccat tcccatccac
     5161 acacactgca cctgagtcat tgcctgaatg ccccgcaaca atacccactc gcaactgagg
     5221 ttgcgatgaa acttgagcat tctccacact ctgcggtgtg aggatcagac ccagccgatc
     5281 ataaagacca cccgccacca gtccacgcga aggcagtgct gtaaagagcg ttgccagcaa
     5341 aatggcaatg gcaagggtgg actgtacagc gcgcaacggg cgcgaacgcc gtaccggact
     5401 ggagggagtc gattccatgt cgatttcgtg taatagtacc ttaacaggga agtaatttac
     5461 aagggtttgc gcatggcagg acgcgaacaa tttttatctt gacgcccctc cccctcaacg
     5521 ttatgatgaa tttttactgg agaaacacct atgacactcg actctgaaac actccgccgt
     5581 gcgatgcgcg catggtcaac cggcgtagca gtcgttactg caaaatatga agatgaacaa
     5641 tacggcatga cggtcaattc attcaattct gtttcactcg aaccgccagt cgtctctatt
     5701 gcacttaaac aattaacgca cactcatacc ctggtcttaa aatccggttt atttgccgtc
     5761 accattctaa gcgctgaaca gaaggagctt tctgatcgtt ttgcagggaa gctccccggc
     5821 gttaccgatc gattcgagga agtgcagatt ggcaggctat ctatagatgc accgatcatc
     5881 aaaaacggac tggcattttt taattgccgc gtgatgagct caacccctat cggtgagaac
     5941 acgttattta tcgcagaagt catcgatgcc cgaggggaag gcgaaggtaa tccgttggtc
     6001 tatcataatc gtatgttttg gaagctgacg caaccctctc ccaaaccctg accccacccg
     6061 tccctgcctt cggcgggaca ccctccccaa atacgacagt cgtatttggg gaggggtatg
     6121 aattatggaa gataaactaa ccctcgaaga acagcaaatc ctgcttcgcc tggcgcgtga
     6181 ggcaatggaa cgcggggtga acggtgagaa gctgtctcct cttaatgaaa gctccctgcc
     6241 tcagaatttg cgcgagaaag gatcttc
//
LOCUS       DASTJP010000488        10188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_989896, whole genome shotgun sequence.
ACCESSION   DASTJP010000488 DASTJP010000000
VERSION     DASTJP010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989896"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFQ23_14205"
     CDS             complement(<1..631)
                     /locus_tag="VFQ23_14205"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HET9907799.1"
                     /translation="MNKYFPISYEDSRARFLQSLHLIQQKWPLTQLESHPLKDFPDLS
                     IDWLWAHARSKKTLLIISTAEHGIEGYVGYAMMKIFIEEFAPRLDPENTGLLLLHGLN
                     PWGMKHHRKVNENSVDLNRNFVVNGNFDPKINPEFYQVQSLINPQRRIQSVGFENIQF
                     WTRVIRALFTAGSAVITKAALLGQHHTPNGFYYGGTSYQEGTIVAMDLYR"
     gene            complement(695..2146)
                     /locus_tag="VFQ23_14210"
     CDS             complement(695..2146)
                     /locus_tag="VFQ23_14210"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET9907800.1"
                     /translation="MEQKAGAQISQRAFIQSILILFVLMMVAGILTLVIPAGSYTRIE
                     VDGRETIDPDSFQFVEKPNYPIWRWFTAPLEVLGSESGLTVIVIIVVLFFAGGAFAVL
                     DKTGTIRAFIGNIVRRFQDRKYTLLYMVCLAFMFLGATLGTFEEVVLLVPVMIALSYS
                     LGWDALVGLGMSILATNMGFSAAIANPYTLGVSQQLAGLPLFSGMWFRVIIFVVIYFI
                     FMAFITAYARKIDRDPKASLVHGEDSAEREKYKSANNSSFTAIDPKQNRAINFFGIFL
                     LFILVIMLAGPFVPAISDYALPIVGILFLIGGLGAGFLSGAGSKTVWQGLREGLIGLA
                     PSVPLILMAASIRYIIDSGGITDTILHAAAEPFTNLGAFPAALVVYALALGIEFFIAS
                     GSAKAFLMIPIVMPLADLIGVTRQTAVLAYIFGDGFSNLAYPTNPVLLICLGLTVVSY
                     PKWIRWTAKLWLWVILATVAFLGIAVAINFGPF"
     gene            complement(2373..2945)
                     /locus_tag="VFQ23_14215"
     CDS             complement(2373..2945)
                     /locus_tag="VFQ23_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9907801.1"
                     /translation="MPRDYTNQSPVSFQRRPHLTRDDGWIRMFLKIAKVGHIASSRDD
                     QPFLNPTMFWFDEENHQIVFHSNVAGRVRSNIETNPKVCLEVSELGKFLPSNVALEFS
                     LQFRSVIVFGNARLVTEPQEARQLLYGLIRKYFPQMTAGQEYREITDKELRATSIYAI
                     QIEEWSGKENWADRADQSDEWTPLDEKWFE"
     gene            complement(2947..3534)
                     /locus_tag="VFQ23_14220"
     CDS             complement(2947..3534)
                     /locus_tag="VFQ23_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907802.1"
                     /translation="MPEGSAPRVELDELLASEYEYITHTANQANEDRARVASFYLIAV
                     GSLVAALFGTQLFDADKFTQSVKVMFSGLFVLLTLLGTSTIMQLARLRGAWYEAMQAM
                     NQIKDFAMKQNPELTDAFRWTKDTLPPKYKKGSVSYFQAVEVSLISALMFGAAVFFLQ
                     QAINPTITLTHWVIASLCGVLTIYIQLFIYRRVLN"
     gene            complement(3550..4815)
                     /locus_tag="VFQ23_14225"
     CDS             complement(3550..4815)
                     /locus_tag="VFQ23_14225"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET9907803.1"
                     /translation="MSSNPGHQELLQKYAEAIVRVGLNIREGQRLIITNATSRGVPPA
                     GRALVHEVTKAAYAAGARYVEVIWGDEEMLRIRLQNAPADSFSEYPKWHVSGVMDMIK
                     NGDALLSVYANDPDALHGLDTDRVNAMQKSHLENYRQIGENVSRNAINWCVVASGSPA
                     WAAKIFPDLEIKEAEEKLWQAIFETTRANLPDPVAAWQDHIQNLKKRSKYLQDKTYSA
                     LHYKGPGTDFTMGLPRGHKWISAQSLAENGVIFTANMPTEEVFTLPDRHRAEGVVSST
                     FPLSYGGSLIEDFSVTFENGRIVKVQAKKNEAILQKLVDTDEGSTRLGEVALVPASSP
                     ISKRGHLFYNTLFDENASCHIAIGRAYRFSLIGGEELTDEEFNTAGGNTSLTHVDFMI
                     GSPQMDIDGIKEDGTREPVMRAGEWAFEA"
     gene            4918..6048
                     /locus_tag="VFQ23_14230"
     CDS             4918..6048
                     /locus_tag="VFQ23_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907804.1"
                     /translation="MLSIEKVDTTNKEQVKRFVELPYRLYKECPQWVPPLYVDSYAYL
                     DRKKHPFHDHSIVEFFLAVRDGQDVGRIAAIENKPFNKYHNTRKANFYFFDCENDPEA
                     ATLLFNRVIAWSKAHELNEVVGPKGMGPLDGYGMLVSGFEHLQTMTMLNYNYPYYEKM
                     IEAQGFTKEVDFVSCYLPADKFQIPPRVEQIAQRVMQRGGLRSQRFKNKRQLIAWADR
                     IGKAYNEAFVNNWEYYPLSAREIDFVVKNIMTIADHRLIKIITHGDEVVGFLFAFHDV
                     SAALQRARGRLFPFGLIDILREIKRTDTVAVNGMGILPKHQGHGGNAILYSEMGHTLL
                     DFKQFRHVEMTQVAETTEQMRADLKNLNGVEYKNHRVYKKEL"
     gene            complement(6099..8372)
                     /locus_tag="VFQ23_14235"
     CDS             complement(6099..8372)
                     /locus_tag="VFQ23_14235"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9907805.1"
                     /translation="MSPQTLNLILLFVLAALYVPVLINLIERRTGQEEAATYLGIFLV
                     IGMLLDIGEGLWRGGRLPIATAQVANDFQLYGALALAFLLTLTVLSFTRRDAKTWLIL
                     GAVWVLGFVIILPNVLRLPAVIWANGRFALTLERLAPTWAVLGWIVFILGGIISVRTA
                     YGRARQPLLRNRLNYWIPVFLLVILNDIFIILRFPLPGNPIRLGAAALATFIIVTHDP
                     ADLREIARRVLTYIITTIVIVAFYIAGFSASQTVFRALPNFDPLLVGAGIALLLSFIF
                     TPLLSLVRRWVNRWLNLQEYHPSRTLHAYSEQISNILDMQKLANVAVGLIIEAMDISR
                     GFLFLVDSELLNEGQRSYRLRAVRNQGERQIRVVNLGQDHPIVRQLTVEGRPLLQYDL
                     DLLPTFRSVTSSEREWFNHLEAEVYLPIFSKREWIGLLALGSKISGNRYTAEDLVTLS
                     ALANQTAVALENARLVDNLVQLNTELRQAYRSLDKANQDLERLDQTKSDFISIASHEL
                     RTPLTTMVGYTEMLIEDITLPTHVHDMLKGIAKGTKRLHEIMDSMFDIAQIDTRTLQL
                     HLISVDTATLVKEVGERLVQSFMERDQTLSINIPPLPMVRADPHLLKKLFQHLFTNAI
                     KFTPNGGRITVTAAPHASHNSEMPSGGVEIIVGDSGVGVDPDVSEIIFSKFYHPGDLN
                     KHSTSKTRFKGSGAGLGLALSKGIVEAHGGRIWVESAGYDEINFPGSKFHVLLPLSNL
                     DKGEETMMGTELDYQLG"
     gene            8420..>10188
                     /locus_tag="VFQ23_14240"
     CDS             8420..>10188
                     /locus_tag="VFQ23_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9907806.1"
                     /translation="MLITKASRDTYQFDSEFFGPSGRLTFDDFSSARRFASRMSENRS
                     QPVSASDVYAMQLIDEAMRMLVRRHAPDVLMSDALSYAEQNVGADSVNATQERFVSEF
                     PPDDVYRGRVKVEDYLERLLASTDSNGRVRTVEELMFIYLHNTNPAVSPLLELVDDQS
                     LEETAYKNLIGSLDSFFSQTAKESAEGGDAPESLFEILSAPAKASPNSLDGQLQFIFD
                     KWGILLGETFIARVVRGMDFLREDVMHEKLAHGDFTPDIPVQTFVGGEYAEYEQYSPD
                     TDWMPRLVLLAKNSYVWLEQLSRKYGTWIKTLDQIPDEELDVLRDRGFTGLWLIGLWE
                     RSRASQRIKQRMGATDAVASAYSLYSYDIAEDLGGWAALENLRGRAWQRGIRLSADMV
                     PNHMGIDSQWVIEHPDWFLSVPYPPYPSYSFKSENLSDDTRVGVYLEDHYYDKTDAAV
                     VFQRRDHYTGDVRYVYHGNDGTSFPWNDTAQLDYSMAEVLEAVIQTILHVARNFPVIR
                     FDAAMTLAKKHIQRLWFPEPGAGGAIPSRSEHGMTRAEFDAAIPEEFWREVVDRVAAE
                     VPDTLLLAEAFWLLEGYFVRTLG"
BASE COUNT         2607 a         2490 c         2568 g         2523 t
ORIGIN      
        1 ggcgatacag atccattgcg acgatcgttc cctcctgata acttgtccca ccataataaa
       61 atccgttggg cgtgtggtgt tgccctaata aggcggcctt tgttatcact gctgaacctg
      121 ctgtgaataa ggctcgtatc acgcgtgtcc aaaattgaat gttttcgaag ccgacggatt
      181 ggattcttcg ttgtgggtta atcaggcttt gtacttgata aaactcggga ttgatcttcg
      241 ggtcaaaatt gccattaact acaaagttgc gattcaaatc cacgctattc tcattgactt
      301 ttcgatgatg cttcataccc catggattca atccgtggag gagaagcagt ccagtatttt
      361 caggatcaag gcgcggcgca aattcctcga tgaagatttt catcattgca tagccaacat
      421 agccttcgat gccatgttcg gcggtagaaa tgatgaggag agtcttcttg cttcttgcgt
      481 gtgcccaaag ccagtcaatg ctgaggtcgg ggaagtcctt tagcggatga gactctagtt
      541 gagtcaatgg ccatttctgc tggatcaagt gcaggctttg taagaagcgc gcgcgtgagt
      601 cttcataaga gattggaaaa tatttattca tcgcaacgag gggatcgtgt aacaatggcg
      661 cttcgactgt gcctccggct ccgctcagcg cgatctagaa gggaccgaaa ttaatcgcca
      721 cggcaatccc taaaaacgcg acagttgcaa gaatcaccca gagccaaagt ttggcagtcc
      781 agcggatcca tttagggtaa ctgaccactg taagtccaag acagataagc agaaccgggt
      841 tcgtcgggta tgcaagattg gaaaagccat ctccgaagat gtaggccagc accgcagtct
      901 ggcgcgtcac gccgatcagg tccgccagtg gcatcacgat gggaatcatc agaaaggctt
      961 ttgcagaacc ggatgcaata aaaaattcta tgcccagcgc gagtgcgtac acaaccagcg
     1021 cagcggggaa agcgccaagg ttcgtaaagg gttcagccgc agcgtgcagg atggtatcgg
     1081 tgatgccgcc gctatcaata atataacgga tgctcgccgc cataagaatc agcggtacag
     1141 atggggcgag cccaataaga ccttcgcgca gaccttgcca tactgtcttt gacccagcgc
     1201 ccgacaggaa ccccgcaccc agcccgccga tcaggaagag aataccaacg ataggcaagg
     1261 catagtctga gatggctggc acaaacggac ctgcgagcat aatgaccaga ataaagagta
     1321 gaaagatacc aaagaagttg atggctcggt tttgttttgg gtctattgct gtgaaagagg
     1381 aattattcgc acttttgtat ttttcgcgct ccgccgaatc ttcgccgtgt accagcgagg
     1441 ctttggggtc acggtcaatt ttgcgtgcat aggccgttat gaatgccata aaaataaaat
     1501 agatcacaac aaaaatgatg acgcgaaacc acatgccaga aaagaggggg agacctgcaa
     1561 gttgttgtga tacacctagg gtatacggat tcgcgattgc tgcagagaag cccatgttcg
     1621 tggcaaggat ggacatgcca agccccacga gcgcgtccca gccgagggaa taagacagcg
     1681 cgatcattac cggcacaagt aagaccactt cctcaaacgt gccgagcgtg gcaccaagga
     1741 acatgaacgc caggcagacc atgtagagta gagtgtattt acggtcctga aatcgccgga
     1801 cgatattacc tatgaaggcg cggatcgtgc ctgttttatc caatacagcg aatgcacccc
     1861 ctgcaaaaaa caatacgacg atgatgacga taactgttag tccgctttca ctgccaagca
     1921 cttcgagagg agcagtgaac cagcgccaga tcgggtaatt cggcttttcg acaaattgaa
     1981 acgagtcagg gtcaatggtt tcgcgtccgt ctacttcgat gcgggtgtag gacccagcgg
     2041 gaataacaag cgtgaggatt ccagcgacca tcatcaacac aaacaaaatc aggatggatt
     2101 gaatgaatgc ccgctgactg atctgcgctc ctgctttttg ttccatgtat ggctcctttg
     2161 tcatttcgtt cccacggcag ttgtactgcc gtatatgttt tttgaagaaa tttagattac
     2221 ttcaccatca gtaaatctgt tattggatag gtttcttctc caagaaggta agcacgcgcg
     2281 ctggaatagg gccattcatc tgccgtcata accaaccctg ctttgacagg atttaagtga
     2341 atttaatcca ttttatgccc cacgaacata atttattcaa accacttctc gtccaaaggt
     2401 gtccattcat cgctttgatc ggcgcggtct gcccagtttt ctttcccgct ccactcttca
     2461 atttggattg cgtaaattga tgtggcacgc aattctttat ctgtaatttc acgatattct
     2521 tggccagctg tcatttgtgg gaaatatttt ctgatcagac catacaaaag ctgacgggct
     2581 tcctggggtt ctgtgaccag tcgggcattt ccaaaaacaa tcacgctccg aaattgaaga
     2641 gaaaactcca gcgccacatt ggatggaaga aacttcccca actcgctcac ttccaaacag
     2701 actttcgggt tggtctcgat attggagcgg actctgccag cgacgttgga atgaaaaacg
     2761 atctgatgat tttcttcgtc gaaccagaac atcgtagggt tgaggaaagg ctgatcatcg
     2821 cgcgaagagg caatatgccc tactttggca attttgagaa acatgcggat ccacccgtca
     2881 tcgcgcgtga ggtgaggtct gcgttggaac gaaacaggag attggtttgt gtaatcacgc
     2941 ggcatattag tttaagactc gtctataaat aaataactga atataaatgg tgagcactcc
     3001 acacaggctg gcgatcaccc aatgtgtcaa agtaatagtc ggattgatag cctgttgcaa
     3061 gaagaatact gccgcgccaa acattaacgc actgatcagt gatacctcaa cggcttgaaa
     3121 atacgaaaca ctgccttttt tgtattttgg tggaagggta tctttcgtcc atcgaaaggc
     3181 atcagtgagc tctggattct gcttcatggc aaaatctttg atctggttca tcgcctgcat
     3241 cgcttcatac caggcaccgc gcagccgtgc cagttgcatg atcgttgagg tacccagcaa
     3301 cgtcaataaa acaaacaagc cactgaacat gactttcaca gactgagtaa atttatccgc
     3361 atcgaaaagt tgtgtgccaa ataatgcagc aaccagagac cccactgcaa tgagatagaa
     3421 cgatgctaca cgggcgcgat cttcattcgc ctggttggct gtatgagtaa tatactcata
     3481 ttcagaggcg agcaactcat ccagttcaac gcgcggagca gacccttcag gcatgagagg
     3541 ctcttatcct tacgcctcga atgcccattc acctgcgcgc atcacgggct cgcgtgtgcc
     3601 atcttctttg atgccatcga tatccatttg gggagagccg atcataaagt ctacatgtgt
     3661 caggctggta ttgccgcctg cggtgttgaa ctcttcatcg gtcaactctt cgcctccaat
     3721 aagtgagaag cgatacgccc gtccgatggc gatatgacag gacgcatttt cgtcaaacaa
     3781 tgtattgtag aaaagatgtc cgcgtttgga gatgggggaa ctggcaggca caagcgccac
     3841 ctcgccgagc cgcgtggacc cttcgtctgt atctaccaat ttttggagga tggcctcatt
     3901 cttttttgcc tgcactttga cgatccgtcc gttctcaaac gtcacgctaa aatcctcgat
     3961 cagactcccg ccatagctga gtggaaaggt tgatgacact accccttccg cacgatgacg
     4021 gtctggcagc gtaaagacct cttccgttgg catattagct gtgaagataa cgccattttc
     4081 tgccagcgac tgagcactga tccatttgtg cccgcgcggt agacccattg taaaatcggt
     4141 gccgggacct ttgtagtgca aggctgaata cgttttatcc tgcaaatatt tggatcgttt
     4201 tttcaaattt tgaatatgat cctgccacgc cgcaacaggg tcaggcaaat tcgcccgtgt
     4261 ggtttcgaaa atagcctgcc agagtttctc ttcggcctct ttgatttcta aatcggggaa
     4321 tattttagct gcccaggctg ggctaccaga ggcgacgaca caccaattga tggcgttacg
     4381 gctgacgttc tcgccgattt gcctgtagtt ctccaggtga gacttttgca tcgcattgac
     4441 gcgatcagta tcgagcccat gcagggcatc cgggtcgttg gcgtagacgg aaagtaatgc
     4501 gtcgccattt ttgatcatat ccatgacgcc acttacatgc cattttggat attcgctgaa
     4561 tgaatccgcc ggtgcgtttt gcagacggat gcggagcatt tcttcgtcgc cccatatcac
     4621 ttccacatac cgcgcccctg cggcatatgc ggctttggtc acttcatgca caagtgcccg
     4681 accagccggt ggaacacctc gcgaggtcgc attcgtgata atgagtcttt gtccttctcg
     4741 aatgttcaac ccaacccgca caatggcttc ggcatatttc tgtagcaatt cctgatgacc
     4801 cgggtttgat gacattgaag actcctgttt gctccgtttt tcgtaagtat agcacgaacc
     4861 atttccgtaa aatttgtctt atacttaccc aaaatcaccg cttcagggaa gttttccatg
     4921 ctcagcatcg aaaaagtgga cacaaccaac aaagagcaag tgaaacggtt tgtggaactg
     4981 ccatacaggt tatataagga atgtccgcag tgggtgccac ctctttatgt cgactcgtat
     5041 gcgtatctgg atcgcaaaaa gcatcctttt catgaccatt cgattgtaga gttctttttg
     5101 gcagttcgag atgggcagga cgtgggtcgt attgctgcga ttgaaaataa acccttcaat
     5161 aaatatcaca acacccggaa agcaaacttc tatttcttcg attgcgagaa tgatccggag
     5221 gctgcgaccc tcctgttcaa tcgtgtcatt gcgtggtcga aggcacacga acttaatgaa
     5281 gtggtggggc caaaaggcat gggtccgctg gatgggtatg gaatgcttgt gtctggtttc
     5341 gaacatctac agacgatgac catgctcaac tacaactatc catactatga aaagatgatt
     5401 gaagcacagg gcttcacaaa agaggtggac tttgtatcgt gctatttacc tgccgataaa
     5461 ttccagattc cgccacgcgt cgagcaaatt gcccagcgag tcatgcagcg tggtggattg
     5521 cggtcgcaac gcttcaaaaa taaacgacag ttgatcgcct gggcagaccg gattggcaaa
     5581 gcctacaatg aggcctttgt gaataattgg gagtattatc ctctttctgc ccgtgaaatt
     5641 gactttgttg taaaaaatat catgaccatt gccgatcatc gcttaatcaa aatcatcacc
     5701 cacggggatg aagttgtcgg attccttttt gcatttcatg atgtttctgc cgctcttcag
     5761 cgtgccaggg gacgtctttt cccgtttgga ctgattgata ttctacgtga gattaagcgt
     5821 actgataccg ttgccgtcaa tgggatgggc atcctgccga agcatcaggg ccatggtggc
     5881 aatgcaatcc tatattctga aatggggcac acgcttcttg atttcaaaca attcaggcat
     5941 gttgaaatga cacaggtcgc agaaacaacc gagcaaatgc gcgcggattt gaagaatctg
     6001 aacggtgttg aatataagaa tcatcgcgtg tataaaaaag aattatgaaa tcaatttgga
     6061 atcaaaaaag agctggcgat tgacgctggc tcttttattt atccgagctg ataatccagt
     6121 tcagtcccca tcattgtttc ttccccttta tccagattac ttaatggcaa caacacatgg
     6181 aacttactgc cagggaagtt gatctcatcg tagcccgcgc tctccaccca gatacgccca
     6241 ccatgagcct ctacaattcc ttttgaaagc gccagtccaa gcccggcacc gctgcctttg
     6301 aagcgcgtct tgctggtgga gtgcttgtta aggtccccag gatgatagaa cttgctaaaa
     6361 atgatttcac tgacatccgg gtccacgcct acgcccgagt cgccgacgat aatctcaacg
     6421 ccaccgcttg gcatttcaga attatgtgat gcgtgaggcg cagcagtgac agtgatgcgg
     6481 cccccattgg gcgtaaactt aatggcgttc gtaaacaagt gctggaagag tttctttagc
     6541 agatgagggt ccgcgcgcac catgggaaga gggggaatat tgatactcag ggtctgatcg
     6601 cgttccatga acgattggac aagacgttca cccacttcct tgaccagggt ggcggtatca
     6661 acggatataa gatgcagttg cagagtacgc gtgtcgatct gtgcgatgtc gaacatggaa
     6721 tccatgattt catgcaggcg tttggtccct ttggcaattc ccttcaacat atcgtgtaca
     6781 tgtgtgggaa gcgtgatatc ttcaattaac atctcggtat agcccaccat ggttgttagg
     6841 ggagttcgca gttcatgcga ggcgatactg ataaagtcag acttggtctg gtcgagtcgt
     6901 tcgagatcct ggttggcttt atctaaggag cgataagctt gcctcagttc cgtgtttagc
     6961 tgaaccaggt tatcgaccaa acgcgcgttc tcaagtgcca cagccgtctg atttgcaaga
     7021 gctgataacg ttacaagatc ttccgctgta taacggttgc cgctgatctt tgaaccgagg
     7081 gcgagcaagc caatccattc gcgttttgaa aaaataggaa gatacacctc tgcttccagg
     7141 tgattgaacc actcacgttc ggaagaagtg accgatcgga atgttggaag caggtcgaga
     7201 tcatattgaa gaagcggacg accctccacc gtcagttgcc gcacgatggg atgatcctgc
     7261 ccgaggttaa cgacccgaat ctgacgttca ccttgattcc gcactgcgcg cagcctgtaa
     7321 gatctttgtc cttcgttcaa tagttctgaa tcgaccagga ataggaagcc gcgcgagata
     7381 tccatggctt cgataatgag cccgaccgcc acgttggcga gcttttgcat atcaagaatg
     7441 ttactgatct gctcactgta cgcgtgcagt gtacgactgg gatgatattc ctgaagattt
     7501 aaccagcgat tcacccagcg tcgcacaagg gatagcaaag gcgtgaagat gaacgagagc
     7561 agcagggcga tgcctgcacc aaccagtaac ggatcgaaat ttggcagggc tcgaaatacg
     7621 gtttgtgacg ccgagaagcc tgcaatgtaa aaggcgacaa tgacaatggt ggtgatgata
     7681 tacgttagta cacggcgtgc gatctcgcgc aaatcggctg gatcatgtgt gacaataata
     7741 aatgtggcta atgccgctgc gcccagccga attgggttgc ccggcagagg aaatctgaga
     7801 atgataaaga tgtcattgag aatgaccaga agaaataccg ggatccaata gttcaaacga
     7861 ttgcgcaaca ggggctgacg agcgcgtcca taagcagtgc ggacgctgat gatcccaccc
     7921 aatataaaaa caatccaacc cagtaccgcc caggtcgggg cgagtcgttc gagagtgaga
     7981 gcgaagcggc cgttcgccca aatcactgca ggaagtcgga gcacatttgg caggatgatg
     8041 acaaatccca gaacccagac agcccccaga atcaaccacg tttttgcatc gcgccgtgtg
     8101 aacgaaagga ctgtcagcgt gagcaaaaag gcgagcgcca gtgccccata taactgaaag
     8161 tcgtttgcga cctgtgccgt tgcaatggga agtcgcccac cgcgccagag tccttcgcca
     8221 atatccagca gcatgccaat cacaagaaaa atgcccagat aagtggcagc ctcttcctgc
     8281 cctgtgcgac gctcaattaa attgatgagc accggcacat acagagccgc aagcacaaag
     8341 agcaatatca aattgagagt ctggggcgac atcgctgcaa agtatactcg aaatcattta
     8401 atgaggttat cattgcaata tgcttatcac aaaagcctcc cgcgacacgt atcaattcga
     8461 ttcagagttc tttggaccaa gtggacgcct gacctttgat gatttttcct cggcccgaag
     8521 gtttgcgtcc cgaatgtcag agaatcggtc tcagcccgtt tctgcctcag atgtatacgc
     8581 gatgcagctc atcgacgagg cgatgcgcat gctcgtacgg cgtcatgcgc cagatgtatt
     8641 gatgagcgat gcgctatcct acgcggaaca aaatgtaggg gcggattccg tcaacgcgac
     8701 gcaggagcga tttgtttccg aatttcctcc cgatgatgtg tatcgaggaa gagttaaagt
     8761 ggaggattat cttgagaggc ttttggcttc aacagacagc aatggacgtg tgcgaactgt
     8821 tgaagagtta atgtttattt acctgcacaa tacaaacccg gcggttagtc cattgctgga
     8881 attagtggac gatcaatcac tggaagagac agcttacaag aatctgatag gttctctcga
     8941 ctcgtttttt tctcaaacgg caaaagaatc cgctgagggg ggagatgcgc cagaatcctt
     9001 atttgaaatc ttaagcgcgc cggccaaagc ttcaccgaac tcgctggatg ggcaacttca
     9061 gttcatcttt gataaatggg gaatactcct gggtgaaacg ttcattgctc gtgtcgtccg
     9121 cggcatggac ttcctgcgcg aagatgtaat gcacgagaag ttggcccatg gcgatttcac
     9181 gcccgatatc cctgtgcaaa cgtttgtcgg cggcgaatat gcagaatacg aacaatatag
     9241 cccagacaca gactggatgc cgcgtcttgt tttactggcc aagaactcct acgtgtggct
     9301 tgagcaactc tcgcgaaaat atgggacgtg gatcaaaacg ctcgaccaaa tccctgatga
     9361 agaattggat gtcctgcgcg atcgaggctt caccggcctg tggctgatcg gcctctggga
     9421 gcgttcacgt gcatctcaac gcattaagca acgcatgggt gcaaccgatg ctgtcgcttc
     9481 ggcctattcg ctttattcct atgatattgc cgaagatctg ggtggatggg cagcattgga
     9541 gaatctaaga ggtcgcgcct ggcagcgcgg catacgactc tccgcggata tggtcccgaa
     9601 tcatatgggc atcgactcgc agtgggtgat tgaacaccca gattggtttt tgtccgttcc
     9661 atatccaccc tatccttcct attcatttaa atctgagaat ctttcagatg acactcgcgt
     9721 gggggtttat ctggaagatc attactacga taagaccgat gcggcagtcg tcttccagcg
     9781 tcgcgatcat tacacaggtg atgtccgtta cgtgtatcac ggtaacgatg ggacatcctt
     9841 cccgtggaat gacactgccc agcttgacta ctccatggcg gaagtgctcg aagcggttat
     9901 tcaaaccatc ctgcacgtgg cgcgcaactt cccggtgatc cgtttcgacg ccgcaatgac
     9961 tctcgccaag aaacatattc agcgcctgtg gttccccgag cctggtgcgg gtggtgcgat
    10021 tccctcccga tctgaacatg gcatgacgcg cgcagagttt gacgcggcca tccccgagga
    10081 gttctggcgc gaagttgtag atcgcgttgc ggcggaagtg ccggatacct tgcttctcgc
    10141 tgaggcattt tggctgcttg aggggtactt cgtccgcacg ctgggaat
//
LOCUS       DASTJP010000489         3395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_426256, whole genome shotgun sequence.
ACCESSION   DASTJP010000489 DASTJP010000000
VERSION     DASTJP010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426256"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="VFQ23_14245"
     CDS             <1..1388
                     /locus_tag="VFQ23_14245"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HET9907807.1"
                     /translation="DLYEIREAIKAAKETCELPVVASVTFTRDDRTLLGDDPTKVART
                     LNEAGVDVIGVNCSGGPAQLLRILKQMKQAVPNGKFWVKPNAGWPEQVGGRIMYPADE
                     DYFGEYALSFRDAGASVVGGCCGTTPQHIAAMRKALDSSVYSSIQVQASEVAETSEVP
                     DATPPTQFAQKLTAGKFAISVEMDPPRGLSTHKLLAGASLLCDAGADVINVADSPMAR
                     MRMSAWAVCDVVQRKVGVETTLHFPTRGRNLLRVQGDLLAAHALGIRNVFVVMGDPTS
                     IGDYPEAMDNYDLVPSGLIKLIKQGFNEGIDHSGTSIGQPTNFFVGAALNLCPQDMDT
                     EIKNLHRKIKAGADFFLTQPVYRNDDGPKLLEAYEAKHGKLEKPILVGILPLVNIRHA
                     TFLHNEVPGIFIPDETRKRIEDAGEDGVKVGVEVAVNLIDQLKAWAGGVYIMPQFHKF
                     DMVAEIIEAVK"
     gene            1523..2293
                     /locus_tag="VFQ23_14250"
     CDS             1523..2293
                     /locus_tag="VFQ23_14250"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET9907808.1"
                     /translation="MLLTIDVGNTNLTLGLYNGNVLGSHWRLATDHNRMPDEYGLQFQ
                     GLLKNAEHTINDVKGICLASVVPQLTSRVVQACREYLECDPFIVDVGIKTGVKVRYED
                     PKAVGADRIADAVAVMYLYGGPACVIDFGTATTFNAITKDGEYLGGAITAGINIATEA
                     LFTHAAKLPRIDLQRPPSVIGRNTVHAMQAGLLFGYVSMVEGMVERFRKELGLAMKVI
                     ATGGLAPIIAQETDVIQIIAPWLTLDGLRILWDLNHPL"
     gene            2296..3267
                     /locus_tag="VFQ23_14255"
     CDS             2296..3267
                     /locus_tag="VFQ23_14255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9907809.1"
                     /translation="MRKPFAFILFLTLLSIGCGGLTFLAGPAPTLVIEPTGTASPPPT
                     PFQPAPASPTLFPTESSTASPSPTLVPTDTFTPTLTLEPQWILQGPNDVIVPILLYHH
                     IGVPQSASPYYISPIDFEGQMYLLREWGYQTISVELLVRAIKQGAELPPKPIILTFDD
                     GGEGTFTNAFPIMQKYDFTGTAYIVFNYVGIRAHMNVDQIHGLYAAGWEIGSHSTSHV
                     DLTVRTERQMDEIVESRRKLQALLNVPIMTFAYPFGAYDSDSVHYAHYAGYIAAMGLG
                     NETLQGDKNLFYLYRMTVDSSQSLESFALRLPWRGDLTNLPVITTVP"
BASE COUNT          864 a          843 c          850 g          838 t
ORIGIN      
        1 gtgatcttta cgagatccgc gaagccatca aagcagcaaa agagacctgt gagctgccgg
       61 ttgtagcatc cgtgacgttc acacgcgatg atcgtacctt gctcggcgat gatccaacca
      121 aagttgcacg gacgctgaat gaagcggggg tggatgtcat tggggtgaat tgttccggcg
      181 ggcccgcaca gctcctgcga atactcaaac aaatgaaaca agccgtgccc aacggaaagt
      241 tttgggtcaa acccaacgca ggctggcccg aacaagtcgg tgggcgcatt atgtaccccg
      301 ccgatgaaga ttactttggt gaatatgctt tgtcatttcg tgatgccgga gcgagcgtag
      361 tcggtgggtg ctgcggaacg accccgcaac atatcgccgc catgcgaaag gctcttgatt
      421 cgtcggtata ttcttcaatt caggtgcagg cttcagaggt agccgaaact tcggaagtcc
      481 ctgatgcaac acctccaaca cagtttgcac aaaaactgac ggcgggcaaa tttgcaatct
      541 ccgttgaaat ggatccgccg cgcgggcttt cgactcacaa acttctcgct ggcgcgtccc
      601 tgctgtgtga cgcgggcgca gacgtgatca atgtggccga ctctcccatg gcgcgcatga
      661 gaatgtctgc ctgggcagtg tgtgatgtgg tacagcgtaa agtgggagtt gaaacaacac
      721 tacactttcc aactcgtggt cgcaacctct tacgcgtgca gggagattta ttggccgccc
      781 acgcgcttgg aattcgcaac gtatttgtcg tcatgggaga tccaacctca attggcgatt
      841 accccgaagc aatggataac tacgacctgg ttccctctgg attgatcaaa ctgattaagc
      901 aggggttcaa tgaaggcatt gatcactcgg gtacatccat cggacagcca accaatttct
      961 ttgtcggcgc ggcgttaaat ctatgtccgc aggatatgga tacagagatc aaaaacctgc
     1021 accgcaagat caaagccggt gctgactttt tcctgacgca acctgtctat cgaaacgacg
     1081 acggaccgaa actgctcgaa gcctatgaag caaagcacgg aaaactcgaa aagcccattc
     1141 tcgttggcat tttgccatta gtgaatatca ggcatgcaac cttcctgcac aatgaagtgc
     1201 cgggaatatt tatcccggac gaaacccgga aacgaatcga agatgcgggg gaagatggcg
     1261 tcaaggttgg ggtggaggtg gccgtcaatt tgatcgatca gctcaaggcg tgggcaggag
     1321 gtgtctacat catgccgcag ttccacaagt ttgatatggt tgcagagatc attgaggcag
     1381 tcaagtaaat tgaagatgcg tatatattct taagcccttc ttatatacct cgttgcacgg
     1441 acggcacaag tcatgtaacg agaaaagatg aacgagtggt aaaatccaca aatcgttcat
     1501 cttttttgtt taaggacttc tcatgcttct tacaatagat gtcggtaata cgaacctcac
     1561 actcgggcta tataacggga atgtccttgg ttcacactgg cgacttgcca ccgatcacaa
     1621 ccgcatgcca gatgaatatg gactgcagtt tcaaggacta ttgaagaacg cggaacatac
     1681 cattaatgat gttaagggga tttgccttgc atcggtcgtt cctcaactta cgagcagggt
     1741 tgtacaagcc tgccgtgaat atctcgaatg tgatcccttc attgtggatg tgggaataaa
     1801 aaccggtgta aaagttcggt atgaagatcc aaaggcagtt ggcgcggatc gtattgctga
     1861 cgccgtagct gtgatgtacc tctatggagg tcctgcctgt gtgatcgatt tcggaacggc
     1921 aacgaccttc aatgccatta caaaggatgg tgaatacctg ggcggagcga tcactgccgg
     1981 catcaacatc gcaaccgaag ccctgttcac ccatgctgcc aaactccccc gcattgacct
     2041 gcaaagaccc ccatccgtta tcggaagaaa cactgtgcat gcaatgcagg ccggactgtt
     2101 atttggatac gtcagcatgg tggaaggcat ggttgaacga ttccgcaagg agctgggact
     2161 ggcgatgaag gtcattgcga cgggtgggct tgcccccatc atcgcacagg aaacggatgt
     2221 aattcaaatt atcgcgccgt ggcttacgct tgatggactg cgaattctct gggatctcaa
     2281 tcatccttta taggcttgag aaaacctttt gctttcatat tgttcctgac tctcttatcg
     2341 attggatgtg ggggattaac attccttgcc ggaccagcac caactcttgt aattgaacca
     2401 acaggcacag cgagtccgcc tcctacgccg ttccagcctg cccccgcttc cccaacgctt
     2461 ttcccgactg aaagctctac tgcatcccca tcaccaacac tcgtcccgac agatacattt
     2521 accccaacgc tcacccttga accgcaatgg atcctgcagg gtccgaatga tgtaattgtt
     2581 ccaattcttc tttatcatca catcggtgtt cctcaaagcg cgagtcctta ttacatttct
     2641 ccaattgact ttgagggaca aatgtacctg ttgcgtgaat ggggttacca gaccatatca
     2701 gtggaattgc ttgtcagggc aatcaaacaa ggtgcagagc tacctcctaa acctatcatc
     2761 ctgacttttg acgatggcgg cgagggtacg tttacaaacg ccttcccaat catgcaaaaa
     2821 tatgacttca caggtacagc ttatattgtt ttcaactacg tgggtatcag ggcacatatg
     2881 aacgtagacc agattcacgg gctgtatgct gcaggctggg agatcggcag tcactccacc
     2941 agccacgttg atttgacagt ccgcactgag cgtcaaatgg atgaaatcgt cgagtcgcgc
     3001 agaaagcttc aggcgctgct gaatgttccc ataatgactt ttgcgtatcc cttcggcgct
     3061 tatgacagcg actcagtcca ttatgcccat tatgccggtt atattgccgc aatggggttg
     3121 ggcaacgaga cactccaggg agataaaaat ctgttttatc tttatagaat gaccgtcgat
     3181 agcagtcaaa gtttagagtc cttcgctctt cggcttccat ggcgcggcga tctgaccaat
     3241 ttacctgtaa tcacaaccgt gccgtagatg cgggaacaaa tctggctctc ggaaagttga
     3301 tagtatagaa agaaggagag agccatggat actaaattgc tttttggtat tggtttaatg
     3361 attttgattc tttcgacctg ttccatcgat gccac
//
LOCUS       DASTJP010000490         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1316369, whole genome shotgun sequence.
ACCESSION   DASTJP010000490 DASTJP010000000
VERSION     DASTJP010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316369"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3560
                     /locus_tag="VFQ23_14260"
     CDS             <1..>3560
                     /locus_tag="VFQ23_14260"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET9907810.1"
                     /translation="ASDTTSGLASFTCTLDGTSLGSCNSIAVNGDGAHTLVLTARDHA
                     GNNRTITQNASLDTQNPLINASLNGTLGSNNWYTNATLNAAASDPAPGSGLSTFEYNL
                     DNSSWISFPVSGVLTLPEGKHSIDVLARDNAGRTVSSSKSFWLDSVSPQVTVDPTGRL
                     GSNNWYTTNLNLSASTSDATSGIDVFEYSLDSGAWLTYSTPLTITDGSHNVSFWSQDA
                     AGLVTQVDGTYKVDTKIPQIAGNLSGTPGTNGWYISDVTLTASASDPASGSGLEAFNY
                     ILNSGTETPYTNALVLSDGEHSVEFNALDKAGLTYSLEQSLKVDTTNPSLSIHTAFPN
                     WIKETVTVNGTASDSGSGLSKVEISTNSGQSWQAMTGITSWSYVWDTSDTPGGIYEVH
                     IRATDNAGLTTRHAINAGVDNYGPKISVPDSWFQWDAVTLDVWDNHSGLSEVRVEISD
                     PEGRWPTRKINLDPENFPLEFKWDRRFGDETVAPLGTYGVKVIAFDKLGNLTRQNASI
                     NILLGILPAGPAATTQPYVRDESTPMPVYASTPIVSSILTQAPVMSAFGSTPEPNLPV
                     EVIPETIPTPRSTPTQTNVLDWLQSVFVPYAGEETVTEIGLAEETQTSREAVASRSNV
                     LWGASAAAMIGAVTAYALEERRKQQEEKAKKAAMEAEEEERREKRQAQKMEKLEAKWA
                     QEQAWEEAQLEAEGREQYLNSERNSSLPVLQPEKSSLEPEEKAWLEKKVQAEKPPKPK
                     DGLQTGLAAYYNAMREGEKETSNSQTNWWESTKSFVSEKIIQPFNSNVYQPYLKPGIE
                     KSQEAMAIGASWLDKNLYQPLIKPAIEKQKQDLANDFEWINENIYQPHLKPAFENTKQ
                     FVISESKWINEKIVQPYVSPILEKVTEKVSNGISWANEKIYQPYLKPVIEKVQEESAK
                     YVSWVNESVYEPYIHPVVTEIKEKVYEPIVEPIVNDINKYIVQPYVKPAVDEASEWWN
                     ETWDQYGEWVHGALDAVGFIPGLGEIADGLNGLIYLGEGRYLDASLTFMSMVPIVGDL
                     NKVAKWSLKAGQEIVETAAEKVTQIVAETFITKVTKESLDEVLEVAIQEAAEELAEKT
                     VREASEELIETTSQTVAEKTLKESTEQFANHVAKESVEEIAEKTVKETSEEVVEAVSQ
                     TVTEQSAKEILDEALANTVEDAIVEEAVEETLEQINEKAV"
BASE COUNT         1091 a          790 c          881 g          798 t
ORIGIN      
        1 gcttcagata ccacctcggg attagccagc tttacgtgta ctcttgacgg cacttcgctt
       61 ggctcatgca attcgatagc agtcaatggt gacggggccc atacactcgt tctgaccgcc
      121 cgcgaccacg ctggaaataa ccgcaccatt actcaaaatg cttcccttga tacacaaaat
      181 cctctgatca atgcaagtct caatggcacg ttgggatcaa acaattggta tacgaatgcc
      241 acattgaatg ccgctgcatc agacccggcg cctggctccg gtttgtccac ctttgaatac
      301 aacctggata acagcagttg gataagcttc cctgtttcgg gtgtacttac actgccagaa
      361 ggtaagcata gcattgacgt ccttgccagg gataatgctg gacggactgt ttcctcctcc
      421 aaatcgttct ggctcgatag tgtttcgcct caggtcacag tagatccgac ggggagattg
      481 ggatcaaaca attggtacac gacaaatctc aacctcagcg catccactag tgatgcgact
      541 tcaggaattg atgtattcga atatagtctt gacagtggtg cctggttaac ctattcgact
      601 cctttaacca tcaccgacgg ttcccataac gtttcctttt ggtcacaaga tgcagccggt
      661 ttagtgacac aagtggatgg tacttataaa gttgatacga agattccaca aattgcagga
      721 aacctcagtg gaactcccgg aacgaacggc tggtacattt cagatgtgac tcttaccgcc
      781 tctgcatcgg acccggcgtc gggatccggt ttagaagcgt ttaattacat cctgaatagc
      841 ggaacggaaa caccctatac aaatgccttg gtactttctg atggagaaca ttcagtcgag
      901 ttcaatgcgc tggacaaagc tggcctgacg tactctcttg aacaaagctt gaaagtggat
      961 accacgaatc cttccttgag cattcataca gcatttccaa attggattaa agaaactgtc
     1021 acagtaaacg gtacagcatc agacagcggc agtggacttt caaaggtaga aatatctaca
     1081 aatagcgggc agtcctggca agcaatgact ggtattacat cctggagtta tgtctgggat
     1141 acatccgata ctccgggtgg aatttacgaa gtgcatatcc gcgctacaga taatgccgga
     1201 ctaacaacca ggcatgctat caacgccgga gtagataact acggaccgaa aataagtgtg
     1261 ccggactcct ggtttcaatg ggacgcagtg accctagacg tgtgggataa ccatagtggt
     1321 ctctctgaag tgcgtgtcga gatcagcgac ccagagggac gctggcccac tcgtaagatt
     1381 aatcttgatc ccgaaaactt tccgttggaa tttaagtggg atcgccgctt cggcgatgag
     1441 actgttgccc cattgggtac atacggcgta aaagtgatcg cattcgacaa attgggcaat
     1501 ttaacacgtc agaatgcttc aatcaacatc ttactgggaa tcctacctgc tgggccagcc
     1561 gcgacaactc agccttacgt acgcgatgag tcaacgccga tgcctgtata cgcatcgacg
     1621 ccaatcgtat cgtcgatact gactcaggcc cctgtaatga gtgcatttgg ttccacgcca
     1681 gaaccaaatc tgccagttga agttataccg gaaactattc ccacccctcg tagcacacca
     1741 acgcaaacca atgtactgga ttggttacag tcggtgtttg tgccctatgc aggcgaagaa
     1801 acagtgaccg agatcggatt agcggaggaa acgcagacca gccgagaagc tgttgcatca
     1861 agatctaacg tcctatgggg tgcaagtgct gcagcaatga tcggtgcggt aactgcgtat
     1921 gcgctcgaag aaaggcgcaa acagcaagaa gaaaaagcca aaaaagctgc tatggaggcg
     1981 gaggaagagg aacgccgcga aaaaagacaa gcgcaaaaaa tggagaagct ggaggctaaa
     2041 tgggcacaag agcaagcctg ggaagaagcc cagttagaag ctgagggacg tgaacaatat
     2101 cttaacagcg aacgaaattc aagccttcca gttctgcagc cagaaaaatc aagtctcgaa
     2161 ccagaagaaa aagcctggct cgaaaagaaa gttcaggctg aaaaaccccc gaaaccaaag
     2221 gatggcctgc agacgggttt agcggcctat tacaatgcca tgcgcgaggg cgagaaggaa
     2281 acatccaatt cacaaacaaa ttggtgggaa agtaccaaat cttttgtgag tgagaagatc
     2341 atccagccat tcaattcgaa tgtctatcag ccatacctca agcccggaat agagaaaagc
     2401 caggaagcga tggcaattgg cgcatcttgg ttggataaaa acctatatca acctcttatt
     2461 aagcccgcaa ttgaaaagca aaaacaggat cttgctaacg attttgagtg gatcaatgag
     2521 aatatttacc agccccatct caaaccggca tttgaaaaca caaagcagtt tgtgattagt
     2581 gagagtaaat ggatcaacga aaagattgtt cagccttatg taagtccgat actggagaaa
     2641 gttactgaaa aagtatcaaa tggcatttcg tgggcaaatg aaaagatcta tcaaccctat
     2701 cttaagccag ttatagaaaa agtccaagag gaatctgcaa agtatgtttc gtgggtgaat
     2761 gagtcggtct atgagccgta tattcacccc gtcgtgactg aaataaagga aaaggtttac
     2821 gagccaatag ttgagccgat cgtaaacgat attaacaaat atattgtcca accttatgtc
     2881 aagcccgctg tggacgaggc atcggagtgg tggaatgaaa cttgggatca gtatggggaa
     2941 tgggtacatg gtgctttaga tgcagtcggt tttattccag ggctcggtga aatcgcagat
     3001 ggactcaatg gtctgattta cctgggcgaa ggtcgctacc tggatgcgtc tcttactttc
     3061 atgtcgatgg tgccgatcgt tggtgatcta aacaaagtcg caaaatggag tcttaaggcc
     3121 gggcaggaaa tcgtggaaac agccgcggaa aaggtaacgc aaattgtggc tgaaactttt
     3181 atcacgaaag tcaccaagga atcattagac gaagtcctag aagtggcaat ccaagaagca
     3241 gctgaagaat tagcagagaa aacggtaaga gaagccagtg aagagttaat agagacgact
     3301 tctcaaacag tagcggagaa gacgctcaaa gaaagtaccg aacagtttgc caaccatgtt
     3361 gccaaagaat cagttgaaga aattgcagaa aagactgtaa aagaaactag cgaagaggta
     3421 gtagaggcgg tttctcagac ggttacggaa caatcggcga aggaaatcct agatgaagct
     3481 ttggcaaaca ctgttgagga tgcaattgta gaagaagctg ttgaagagac tctggaacaa
     3541 atcaatgaaa aagctgtcca
//
LOCUS       DASTJP010000491        12414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1062483, whole genome shotgun sequence.
ACCESSION   DASTJP010000491 DASTJP010000000
VERSION     DASTJP010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12414
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062483"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFQ23_14265"
     CDS             <1..744
                     /locus_tag="VFQ23_14265"
                     /inference="COORDINATES: protein motif:HMM:NF028211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HET9907811.1"
                     /translation="KMNEAMVHQFLDQINSAHPRLQLSIYDIQQVTWGFLPVNRADAN
                     KQPVKLARDGLAIDHQKKDGISGLISILGVKYSTARVVAEQAVNLAVNQLGVKANKCQ
                     THMTPIKGGKIDDFRAFLRRALLKVPRVINERTINHLVYTYGSEYHGLVETMLRQPAL
                     ARRIDWPWPVSLAEVKHAVRHEMALTLADVIRRRTELGSTGLPSMATLQKCASIMASE
                     NQWDFNRQQQEIDLVIQAYRFKQAENYAN"
     gene            734..1546
                     /locus_tag="VFQ23_14270"
     CDS             734..1546
                     /locus_tag="VFQ23_14270"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9907812.1"
                     /translation="MPIDRLPWQIEMFRRSIKKQQKLKALLDFVGETTGKKCLLVTCG
                     DNSGALNWHFRTHGGEWTWADVSGENNDQISSLLNEPVYAFKEDEFPLPSEKFDCVVS
                     IDVLEHLKLDQPFLRELKRVLKPGGKAVVTVPNGDERLLANKIKWRIGMTPEMYGHTR
                     AGYTVSELSDALRRSGLEPKDTGGYSRLATEMIELVINFGYVRVLAKRHGVAKPGHIA
                     PVSAGELKTHGLAYRIYSFIFPIARAISLLDHLMPAGMNNAVIVSAEKEGQK"
     gene            1543..2520
                     /locus_tag="VFQ23_14275"
     CDS             1543..2520
                     /locus_tag="VFQ23_14275"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9907813.1"
                     /translation="MRVLITGAAGFIGSHLVDSQLEHGHQVRAVDLHLEPLMHHEAHP
                     RLEAIRGDITQKDLVQKLVEGVDLIYHLASAHLDVSLSDEHYRRVNVGGTLLLMEAAR
                     KAGVGRFVHCSSVGVIGDVKSPPADETTECYPINIYERTKLEAEDVVLQFARRTGFPI
                     VVVRPAWVYGPRCPRTAKLFRTISKGRFPIFGSGRNMRHPVYITDAICGLELCVEKPD
                     IDGEVFIIAGEAPVETRALINMISKELHVRKPNIHLPIFLGQAAGLTMELAFKLLGRR
                     PPFSRRSMDFFLKHNAYTIAKAQSQLGYRPQVDLLTGIKKTVQSLTASL"
     gene            2532..3338
                     /locus_tag="VFQ23_14280"
     CDS             2532..3338
                     /locus_tag="VFQ23_14280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907814.1"
                     /translation="MNEKLASTVEEVHDYWNTHTLGFQYVTNYRVKPGTPEFFAHIRP
                     WMNPYKFTWIMERIDREAKLLKGKHLLEIGCGMGYDSLEFLKRGVRVTATDLTPNAVE
                     LTRKHFEIEKVKAEETRTANALELPFENDTFDAVWSNGVLHATGDTRRAIQEARRVLK
                     PGGRAIISHFYRKPSWMYAINRLGRENIEYKEEDPPVNEFYTDEEVLDFFQGYEVIEA
                     VHEHERLLPARRDGIKSFLYHKLLMPAYNRLPLPVAMKYAYKISVTAIKT"
     gene            3348..5282
                     /gene="asnB"
                     /locus_tag="VFQ23_14285"
     CDS             3348..5282
                     /gene="asnB"
                     /locus_tag="VFQ23_14285"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9907815.1"
                     /translation="MCGICGKYSPTGVQSAELNHMLDTIAHRGPDDSGCYVRGNIGLG
                     NRRLSIIDLQSGKQPISNEDRTIWVVYNGEMYNFPQLRRQLESKGHTFHTNSDTEAIV
                     HLYEEFGEGCVEHISGMFAFALWDEREQKLLLARDRIGQKPLFYSLDGLDFYFGSEIK
                     SSLALHSQTTELDMLAMHDYLSLRFISPPRTIFKHIQKLAPAHTLVFQYGQIRLRRYW
                     QLSFREKLQISESEILDALQQQIRVTVSSHLLSDVPIGAFLSGGLDSSLIVAVLAQDL
                     GLKPQTFSIGVDESDFDETPYARMVSEHHQTNHVEERVAANLVTSLPELIWHLDEPSD
                     PIAACMFQAARLASRHVKVVLGGDGGDELFAGFDRYVGGRYIDLYNAMPLNLRQSLIG
                     PILERLPDSFTYKSFTQKLRWVHHLSLQSTAAEQYAEATCFFRFTHEGKRSLYSSQLW
                     KELGNRNSTVAITEPFNQAHADDLLDRMLYTDFITRLPEHSLVLTDRMSMAHGLETRS
                     PFLDHELVEFLAKVPAKIKVQNNQPKYLMRKLAANYLPTPILQREKQGFMLPIAYWFR
                     TDLFPVASQMLQSSYFVKEGWFKTETIQKLLDEHRSRRYDHHVRLWMLLTLELWHQLY
                     IQGVDREVLREKLQELCLPQ"
     gene            5842..7590
                     /locus_tag="VFQ23_14290"
     CDS             5842..7590
                     /locus_tag="VFQ23_14290"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET9907816.1"
                     /translation="MIVLGIIDSKPSSAAILKDGQILSAMAEERLCRMKMASGMPRKA
                     IHQVMADASISAKDIDVVAVAQKVSVFEPEPIPWNGWFDANQAGKPRPFEKISGTLAP
                     IAGYIPFAHKAHHQLKDLLSRKRLAQIPELLEREYAITAPVKYYDHHFCHATSAYFTS
                     NYRKALVVTLDGGGDGLSGSIYQGEGGRLTRLSVVDSFNSLGNFYSYITHLCGFKAES
                     HEGKITGLAALGKPSYIGILREFVSYEEPGQIQYSVPMYHRSALRQIAARLPADFDKA
                     DLAASVQLLLEEIGIEFIRFWLRKTGNRSISVAGGVFSNVKFNQRVHELEEVDNFFVH
                     PAMDDSGLAVGGAFAALSEEPGADPGRLLQRLKNVYFGTSYGNEEIRKCIEGFGLKST
                     YEPQITTVVARLLAEGYVVARFTGRMEYGPRALGHRSILYQTTDPSINDWLNAHLLRT
                     EFMPFAPATLQDYADECFDRLNGARDAARYMTITFKCTEKMRVQSPGVVHVDGTARPQ
                     ILDPETAPDLYQIAKEYHKLTGIPSLINTSFNMHGEPIVCSPQDALRSFQQGKLDYLA
                     IGNWLVVNPELQKNRK"
     gene            7623..8741
                     /locus_tag="VFQ23_14295"
     CDS             7623..8741
                     /locus_tag="VFQ23_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9907817.1"
                     /translation="MRIGIMLRSITEMGGVGVYTRNIVQELLNLDKKNEYVLFYANRS
                     DLGMFSHLENVNEVWVKARHKALWDQIAIPRVCRREKIDVLFHPKFTVPLLAPCKAVM
                     VVHGADWLIPEQAQFYPWWDVKYMRIMLPFYFRKASVVISVSQETTDNFNQILRLPVD
                     KIHTIYFAPARHFKRITDPDVLQRVRARYQLPDKFILTLTKRKGGERKNLGQVLRAYA
                     GYHEKVLSPHKLIVGGKDCHLFRAEYEIPEDGYGKDILFPGWIDQADLPAVYSLADLY
                     LYPSNLEAFPIPLTEAMACGTPILTSNVNGLKEIAGDAALLVEPNETERISMGMAEIL
                     SNAEMRESLSRRGLKRSSLFSWDRCAEGTLELLEQAAR"
     gene            8738..9586
                     /locus_tag="VFQ23_14300"
     CDS             8738..9586
                     /locus_tag="VFQ23_14300"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET9907818.1"
                     /translation="MIDLIQDLDLQVLTGAVVIVAPHMDDEALGCGGLISKLPYKDFI
                     HIIYATDGMKSPAPIISGRDKITPDLGKNRMQESIAAMKVLGVPEQNLHFLCLPEAQL
                     KNHLSALKNLLREKISSISPTHIFIPFRYDRHADHLAVNRAVTSDLGYYNSPVQLIEY
                     FIYYRWRLMPGRDIRRYIRSEFLFKLEIKDVAQQKRQALDCFTSQTTIYYSWQTRPIL
                     TSILLNEECQNPEIFLVSNHASVGAAVFSNSVLWIRIVHRLEPFLQRWKYQIGATLKR
                     FLQTNV"
     gene            9579..10388
                     /locus_tag="VFQ23_14305"
     CDS             9579..10388
                     /locus_tag="VFQ23_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HET9907819.1"
                     /translation="MSDHQVEVNRPIRVVMFGGGPKLKQDAKKFILRLEEDPEIEFLG
                     AFCQAQSQSLVGVLFDLWKRRRWLAIPLFFILVINKATQILVHPREEIAFQKSLGKIL
                     DRIHFVTDIHSQTVIAQVRALNPDLGLIYGSPILKPELFEIPRLGTLGIHHGKVPEYR
                     GNKTTFWAMYNGENVAGVTIQKVNQGLDTGSIVKSAEVDTQGRTYQAVVHDLEKVGLD
                     LFLQAILEVKDGTAEYKPQFGVKGTLYRNPKPLDFLKFWCRQLKRRVRMAK"
     gene            10385..11593
                     /locus_tag="VFQ23_14310"
     CDS             10385..11593
                     /locus_tag="VFQ23_14310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9907820.1"
                     /translation="MKICIFTETYYPVIGGGETQSRLLAEKLIDDGHSVIILTRRSDK
                     ALRKHEHYGELDVFRLAPVGSGQLKKWGLVLSSIPALIRLRDQYDLIFVSGYRIIGLT
                     AVLIGKLFRKPIVLKADSQGEMSGEFFESGLRNFRISRSSLVFRLFLKIRNAILKKAD
                     AFSAISPDIAAEWTSSGVSLHKIRLIPNGVDTDCFVPASAEQKTILRQKLSLPETALI
                     AIYTGRLVSYKGLPLLLKAWNEIRRKHQNALLILAGTGGLDIHNCEDALREYVRVNRL
                     EQYVLFTGAVQNVAEYLQASDLFAFPTEDDAFPSSVVEAMACGLPVVTTPVGAIKHIV
                     KDGDTGVLVQPSNFTQLFEALDIILSNKEIGSRLAKTASRSVQDLYSAEIVSKNYLTL
                     FQGLIDPTKA"
     gene            11692..>12414
                     /locus_tag="VFQ23_14315"
     CDS             11692..>12414
                     /locus_tag="VFQ23_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907821.1"
                     /translation="MPFVAQREQWTIGIYRGNSPFHFNEFQGWINPLFRAEDITDVSA
                     KFVADPFLIKEKETWNLFFEVYDNDTQQGDLAVATSNNTWKWNYEKVILDEPFHLSYP
                     YVFKSEDRYYLIPESFEDNSIRLYKADEFPTKWSFVKTLVSGRDYVDNSIVFYNNKWW
                     LFSSVTSNDKLYLHYADSLEGKWKEHPQSPIVVGDVHKSRPSGRLLVYDGRLFRFTMD
                     IDPPVGTHQVMVYEITEITSTSY"
BASE COUNT         3234 a         2863 c         3030 g         3287 t
ORIGIN      
        1 aaaatgaacg aagccatggt ccaccaattt cttgatcaaa tcaatagcgc tcatcccaga
       61 ctccagcttt ctatatacga tatccagcag gtcacctggg gattcctgcc agtaaatagg
      121 gcagacgcaa acaagcaacc cgtcaagctg gcgcgtgatg gtctcgcgat tgaccaccag
      181 aaaaaagatg gaatatcagg tctgatcagt attcttggtg ttaagtacag cacggcgcgt
      241 gtcgtcgctg aacaggctgt aaatctggcg gtgaaccagc ttggcgtgaa agcaaacaaa
      301 tgtcagactc atatgacgcc aattaaaggt gggaaaattg atgattttcg tgcttttctg
      361 cgaagggcct tacttaaagt tccacgcgtg attaatgagc gtacaattaa tcatttggtg
      421 tatacctacg gctcagagta ccatggactg gttgaaacga tgcttcgaca gcctgcgtta
      481 gctcgcagaa ttgactggcc ctggccagtt agtttagcag aggtgaaaca tgcagttcgc
      541 cacgaaatgg cattgacact cgccgatgta attcggcgac gaacagaatt gggttcaaca
      601 ggtctgccgt ccatggctac cttgcaaaaa tgtgccagca taatggctag tgaaaaccaa
      661 tgggatttca atcgtcagca acaggagatc gatttggtca ttcaagccta cagattcaaa
      721 caggcagaaa actatgccaa ttgatcgact tccttggcag atcgaaatgt ttcgaaggtc
      781 gatcaaaaaa cagcaaaagt tgaaggccct tttagacttc gttggcgaaa caactggtaa
      841 aaaatgctta ctggtcacct gcggcgataa cagcggagcg ctgaactggc acttccggac
      901 ccacggcggt gaatggacgt gggccgacgt gtctggtgaa aataatgatc agatctccag
      961 ccttctcaat gaacccgttt acgcattcaa ggaagatgag tttccgcttc cgagtgaaaa
     1021 gttcgactgt gttgtttcca tagatgtgct agagcatctc aaactagatc agccttttct
     1081 acgagagcta aaaagagttc tcaagccagg cggcaaagcg gttgttaccg tcccaaatgg
     1141 ggatgagagg cttctggcaa acaaaatcaa atggcgaatc ggcatgacgc ccgagatgta
     1201 cgggcatacg cgcgcaggct atacagtttc cgaactgtct gatgctcttc gcaggtcggg
     1261 gctggagccg aaggatactg gcgggtattc gcgtttggcg acagagatga tcgagttggt
     1321 gattaatttt ggttacgtgc gcgtgcttgc taagaggcac ggagttgcga aacctggtca
     1381 cattgcgccc gtttctgctg gtgaacttaa gactcacgga ttggcctatc ggatatattc
     1441 attcattttc ccgatcgcgc gcgcaattag tttactggat cacctgatgc ccgcagggat
     1501 gaacaatgca gttattgtga gcgcggaaaa ggaaggtcag aaatgagagt tctgatcact
     1561 ggagctgccg gtttcattgg aagccacctg gtggatagcc aactcgaaca cggccatcag
     1621 gttcgtgcag tcgaccttca ccttgaaccg ttgatgcatc atgaagcaca ccctcgtctg
     1681 gaagcgattc gtggtgatat cacccaaaaa gatttggtcc aaaagcttgt tgagggtgta
     1741 gatcttattt atcatcttgc aagtgctcat cttgatgtga gtctttcaga tgaacattat
     1801 cgccgtgtga acgtgggtgg cactctgtta ttgatggagg cggcaagaaa ggccggcgta
     1861 ggccgctttg tgcactgcag cagtgtgggc gtgatcggtg atgtgaaaag tcctcccgca
     1921 gatgagacca cggaatgtta cccgatcaac atctacgaga gaaccaagct cgaagcggaa
     1981 gatgtggtat tacagtttgc cagacggaca ggctttccca tcgtggttgt tcgcccggct
     2041 tgggtgtacg gaccgcgatg cccgcggact gcaaaattgt tccgcacgat ctcaaaggga
     2101 cgcttcccga ttttcggtag cggcagaaat atgcggcatc cagtctatat cactgacgca
     2161 atctgcggcc tggaattgtg tgttgaaaag ccggatattg atggcgaagt gtttattatc
     2221 gccggcgaag ctcccgtaga aaccagggct ctcataaata tgataagcaa ggaactccat
     2281 gttcgaaaac ccaatatcca tcttccaatt tttctcggtc aggcggcagg tttgactatg
     2341 gaactcgcgt ttaagcttct tggacggcga cctccttttt cgcgccgaag catggatttt
     2401 ttcttgaaac acaacgcgta tacaattgct aaggctcagt ctcaattggg ttatcgcccg
     2461 caggtggatt tactcacggg catcaagaag actgtccaaa gtttaactgc tagcctgtag
     2521 gataacaaaa catgaatgaa aaattagcct caacagttga agaagttcac gactattgga
     2581 atacacatac cctgggtttc cagtatgtga ctaattacag ggtaaagcct ggtactccgg
     2641 aattctttgc ccatatccgt ccctggatga atccctacaa gttcacatgg attatggaac
     2701 gtattgatcg ggaagcgaaa ctgttaaagg gaaaacacct gctcgaaatt ggctgtggca
     2761 tgggatacga cagtcttgag ttcctaaagc ggggagttcg ggtcacagcg accgacctga
     2821 cacctaacgc agtagaactg actcgcaagc attttgaaat cgagaaagtc aaagcggaag
     2881 agacacgcac tgcgaatgcg cttgaacttc cattcgaaaa tgatacgttc gacgcagtct
     2941 ggtcgaacgg cgtgttgcat gccacgggcg acacacgccg cgccattcaa gaagcccggc
     3001 gagtcctcaa gcccggcggg cgtgcaatca tttcgcactt ctatcggaag ccttcttgga
     3061 tgtatgcgat taatcggtta ggtcgtgaga atattgaata caaggaagaa gatccgccag
     3121 tcaatgaatt ctacaccgat gaagaagtcc ttgatttctt ccagggttat gaagtgatag
     3181 aggccgttca tgaacatgaa cgtctgcttc ctgcgcggcg cgacggtatc aagtcctttc
     3241 tctatcataa attgctcatg cctgcttata acaggcttcc cttaccggtt gcaatgaagt
     3301 atgcatataa gatttcagta actgcgatca agacttagaa ctgttatatg tgcggaattt
     3361 gcggtaaata cagtccaact ggcgttcaat ctgcagagct gaatcacatg ctcgatacga
     3421 tcgcgcatcg tggtcctgat gattcaggtt gctatgtgcg cggcaacatt ggcttgggca
     3481 atcgccgtct cagtatcatc gatttgcaaa gtggaaagca gcccatcagc aatgaagacc
     3541 gcacaatctg ggttgtctat aatggcgaga tgtataactt tccgcagctc cgcaggcaac
     3601 ttgaatcaaa aggtcatacg ttccacacga attctgatac cgaagccatt gtgcatcttt
     3661 acgaggaatt tggtgaggga tgtgtagaac atattagcgg catgtttgcc ttcgcacttt
     3721 gggatgaaag agagcaaaaa ctcctgcttg cgcgggatcg catcggacaa aagccgctct
     3781 tctattcatt ggatggtttg gatttctatt ttggctcgga gataaagtcg agcctggcac
     3841 ttcattccca aactacggaa cttgatatgc ttgcgatgca cgactatctt tccttgcgtt
     3901 ttatctctcc cccgcgcacg atcttcaagc atatccagaa actcgcgcct gcccacacac
     3961 ttgtatttca atacggacag ataaggctac gccgctactg gcaactttcc ttccgcgaaa
     4021 aacttcaaat atcggagtcc gaaatactgg atgcgctgca acagcagatc agagtgacag
     4081 tgagttcaca tttgctcagt gatgttccca ttggggcatt cctcagtggc ggactcgact
     4141 ccagcttgat cgttgctgtt ctcgcccaag accttggact caagccacag accttctcca
     4201 taggcgtgga tgaaagtgac tttgatgaaa ctccatatgc gcggatggtt tcagagcatc
     4261 atcagacgaa ccatgttgag gagcgcgttg ccgccaatct tgtcacatcc cttccggaac
     4321 tgatctggca cctcgacgaa ccctcggatc ccattgccgc ctgtatgttt caggcagcgc
     4381 gcttggcatc tcgacatgtg aaggtagtgc tagggggcga cggaggcgat gaactctttg
     4441 ctggatttga ccgttatgtt ggcgggcgct atatcgattt atacaatgca atgccgctca
     4501 atttgcgaca aagcctgata ggaccaatct tggagcgctt gcctgatagc tttacgtaca
     4561 aaagttttac acagaaactt cgttgggtac atcatttgtc cctccagtcc accgccgcag
     4621 aacagtatgc agaagcaact tgtttttttc gtttcacaca cgaaggaaaa cgctctcttt
     4681 actcttctca actctggaag gaactcggca atagaaattc cacggtcgca atcactgagc
     4741 cttttaatca agcccacgcc gacgacttgc tggatagaat gctttacacc gactttatca
     4801 cccgtttgcc agagcattcc ctggtcctaa cggatcgaat gagcatggcg cacgggctgg
     4861 agactcgctc gccctttctc gatcatgaat tagttgaatt tctcgccaaa gttccggcaa
     4921 aaatcaaggt acaaaataac cagccaaaat atctcatgcg caaactcgct gcgaattatc
     4981 ttcctacccc gatccttcag cgcgaaaaac agggctttat gctgccgatt gcgtattggt
     5041 ttcgaaccga cctctttcca gtagccagcc agatgcttca aagttcttat tttgtgaagg
     5101 aaggctggtt caagaccgaa accatccaga aattattgga tgagcatcgc agtcgtcgct
     5161 acgatcatca tgtgcgttta tggatgctgt taactcttga gctttggcat caactttata
     5221 tccagggcgt tgatcgcgaa gtcctaagag aaaaattgca ggaattatgc cttccccaat
     5281 agcgcatctt ggcatggggt atgcaatata tcgtttttac aaagataaat tgccacctga
     5341 ccggaggcgc gtttggaaat tgccttttca agtgatcctg ctaactggaa tgtcgatttt
     5401 gccagatctg gatgtaattc cggcaattgt cttcagagac atgaagactt atcataataa
     5461 tttctcgcac agcatccttt ttgcagttcc ggtagctcta ttgattgctg gaatcttcca
     5521 taagctttat cgatctaact tttggttgtg gttcctgatt tgcctcgtct catatgattt
     5581 gcatattctc atggatgtgc tgacagcaga aaggggcgta atgttgtttt ggccatttac
     5641 ccagaatcgt tttgcttctc ccataaagat tttctatgga ctacagtggg gcctcgggtg
     5701 gtttagcatt tggcacttat ggaccattct tactgaatca ctctttgtga tgataatgat
     5761 tggtgcccta aattatcttg ggaaccgaag aaaaacgtac acgccactct ttcacagacg
     5821 aacgaaagaa agacgcaaaa tatgattgtc ctaggtatta tagacagcaa accctcgtct
     5881 gccgcaatct tgaaagatgg gcagattctc tcagctatgg ccgaagagcg cctttgccgg
     5941 atgaagatgg caagcggcat gccccgaaag gccattcatc aagtcatggc agatgcgagt
     6001 atttcagcaa aggatattga tgttgtggca gttgcccaaa aggtgagtgt tttcgagccg
     6061 gaaccaatcc catggaatgg ttggttcgac gctaatcaag ctgggaagcc gcgtcccttt
     6121 gaaaaaatca gcggaacact ggccccgatt gccggatata tcccatttgc ccacaaggcg
     6181 catcatcagc tcaaggacct tctctcgcgc aaacgactgg ctcagattcc cgagttatta
     6241 gaaagggaat atgcgattac tgcaccggta aagtattatg atcaccattt ctgtcatgca
     6301 accagtgcct atttcacgag taattaccgg aaggcgctgg tggtcacgct tgatggtgga
     6361 ggggatggat tgtctgggag tatctatcag ggtgaaggag gtaggctgac tcggctctcg
     6421 gtagtcgata gtttcaattc actagggaac ttttactcct atatcactca tctgtgtgga
     6481 tttaaggcgg aaagccatga gggcaagatc accggcttgg ccgccttagg aaaaccttct
     6541 tacatcggca tcctgcgtga atttgtcagt tatgaggaac ctgggcagat tcaatattcc
     6601 gtgccaatgt atcaccgttc cgcattgaga cagattgccg ctcggctccc cgctgatttt
     6661 gataaagcgg acctggcagc cagtgttcaa ctcctgctgg aagaaatagg catcgagttt
     6721 atccgtttct ggctgaggaa aacaggaaat cgttccattt ctgtcgctgg cggtgtattt
     6781 tcgaatgtca agttcaatca gcgagttcat gagcttgagg aggtcgacaa cttcttcgta
     6841 cacccggcca tggatgatag cggactggct gttggtggtg cctttgctgc actttctgag
     6901 gaacccggtg ccgatcccgg gcgtctgctc caacgactga agaatgttta tttcggaacc
     6961 agctatggga acgaggagat tcggaagtgt attgaagggt ttggacttaa atcgacatat
     7021 gaaccgcaaa tcaccactgt cgttgccagg ttgctggctg aaggatatgt ggtcgctcgc
     7081 tttaccggca gaatggaata tggaccgcgc gccctgggcc accgctctat cctatatcaa
     7141 accactgacc cctccattaa cgactggttg aatgcgcact tgttgcgcac agaattcatg
     7201 cctttcgccc cggccacctt acaggattat gctgatgaat gctttgatag attgaatggg
     7261 gcccgtgacg ccgcgcgcta tatgacgatt acgttcaaat gtaccgaaaa gatgcgtgtt
     7321 caatctcctg gtgtcgtcca cgtcgatggg accgctcgtc cgcaaattct tgatccggag
     7381 accgcccctg acttatatca gatcgcgaag gaatatcata agctcacggg gattccatct
     7441 ctgattaaca ccagttttaa catgcacgga gagcccattg tttgtagccc tcaagacgca
     7501 ttacgttcct ttcaacaggg caaactagac tatctcgcaa tagggaactg gcttgttgtc
     7561 aatccggaat tgcagaaaaa tagaaagtag taggcactga tttttattcg gtgaacaatt
     7621 acatgcgcat tggcatcatg ctgcgatcca taactgaaat gggtggagtg ggagtgtaca
     7681 ctcgcaacat tgttcaggaa cttctgaatc tcgacaaaaa aaacgaatat gttttgttct
     7741 atgccaaccg atccgatctg ggtatgtttt cgcatcttga gaacgttaat gaagtctggg
     7801 tcaaggccag acacaaggca ctatgggacc agattgccat tccccgtgtg tgccgccggg
     7861 aaaaaatcga cgttcttttc caccctaaat tcactgtacc actccttgct ccatgcaaag
     7921 ctgttatggt agtccatggt gcagattggc taattccaga gcaggcgcaa ttctacccat
     7981 ggtgggatgt gaaatacatg cggatcatgc tgcctttcta cttcagaaag gcgtcggttg
     8041 taatctcggt ttcgcaggag acgaccgata acttcaacca aattctaagg ttgccggtag
     8101 acaaaattca tactatttat tttgcacccg caaggcattt taagcgaatt actgatcctg
     8161 atgttttgca acgagttcga gcgcgttatc agctccccga caaattcatc ctcacgttaa
     8221 ccaagcgcaa gggtggcgag cgcaagaatc tgggacaggt cctcagagct tatgccggtt
     8281 accatgaaaa agtgctttca cctcacaagc tgattgttgg tggaaaggat tgtcacttgt
     8341 ttagggctga atacgaaatt ccggaagacg gctacgggaa agatattctt tttcctggct
     8401 ggatcgatca ggcagacctt cctgcggtgt acagtttggc cgatctttat ctctatccat
     8461 ccaatcttga ggcattccct attccgctca ctgaagccat ggcctgcggc acacctattt
     8521 tgacatcgaa cgtcaatgga ttgaaggaaa ttgcagggga cgcggcacta ttggtggaac
     8581 cgaatgaaac agaacggata tccatgggta tggctgaaat tctttctaat gccgaaatgc
     8641 gagaatccct ctctcgtaga gggttgaagc gttcctctct tttcagttgg gaccgctgcg
     8701 cagaaggcac tctggaactc cttgaacagg ctgccaggtg attgacctaa tacaggatct
     8761 tgatctacaa gtcctgacgg gcgcagtagt aatagtagcg ccccatatgg atgatgaagc
     8821 tcttggttgc ggcggattga tttccaaact gccgtataag gattttattc acattattta
     8881 tgccacagat gggatgaaat ctcctgcacc catcatctct ggacgcgaca aaataacgcc
     8941 tgatcttgga aaaaatcgta tgcaggaatc gatcgcagct atgaaggtat tgggcgtccc
     9001 ggaacagaat ctacattttc tttgtttgcc agaagctcag ctaaaaaacc acctgtccgc
     9061 attgaagaat ttgctcaggg aaaaaattag ttcaatatca ccaacgcata tttttatccc
     9121 atttcgatat gaccgacatg ccgatcactt ggccgtcaat agagcagtga catcagacct
     9181 gggatactat aatagcccag ttcaacttat tgaatatttt atctattacc gctggcggtt
     9241 gatgccagga cgtgacattc gaagatatat ccgttcggaa tttctcttca aattggaaat
     9301 aaaggatgta gctcagcaaa agcggcaggc gctggattgt tttaccagtc agactacgat
     9361 ttattattcc tggcagaccc gccctatctt gacatcgatt ctgttgaatg aggagtgcca
     9421 gaacccggaa atttttcttg tttcaaatca tgcctcggtt ggggcagcgg tcttttcaaa
     9481 ctctgtgctt tggattcgca tcgtgcatcg ccttgagccg tttctacaaa gatggaaata
     9541 tcaaattggc gccacgctga aaaggttcct tcaaactaat gtctgatcat caagttgagg
     9601 tgaaccggcc gatccgagtg gtgatgttcg gcggaggacc caaattaaag caggatgcca
     9661 aaaagtttat cctgcgattg gaagaagatc ctgagattga atttctgggt gcattctgtc
     9721 aagcacagtc ccagtcattg gtcggggtgt tatttgactt gtggaagcgt cgaagatggc
     9781 tggcaatccc gttgtttttt attttggtta tcaacaaggc aacacagatt ctcgtccatc
     9841 ctcgtgagga aattgctttt caaaaaagcc tgggaaaaat cctggaccga atccattttg
     9901 ttaccgacat ccattctcag acggtaatag cgcaagtgcg tgcgctcaat cctgatctgg
     9961 ggctgattta cggcagtcct attttgaaac cggagctctt tgaaattcca aggcttggca
    10021 ctttaggtat tcatcacggt aaggtccctg aatacagagg caacaaaaca acattctggg
    10081 cgatgtataa cggtgaaaac gtcgctggtg tcaccataca gaaagtcaat cagggattgg
    10141 acaccgggag cattgttaaa tcagccgaag ttgacactca gggccgaaca tatcaggcgg
    10201 tcgttcatga tttagaaaag gtcggtctcg atttatttct gcaggcgata ctcgaggtca
    10261 aagatggaac cgcggaatac aaacctcaat ttggtgtaaa aggaacacta tatcgaaatc
    10321 caaagcctct tgactttctt aagttctggt gcagacaact caaaagacga gtgagaatgg
    10381 cgaaatgaag atctgcatct tcactgagac ctattatcct gttatcggcg gaggcgagac
    10441 ccaatctcga ttgcttgccg aaaaattaat tgacgatgga cattccgtca ttatactgac
    10501 tcgacgatcg gataaagcat tgagaaaaca tgaacattat ggtgagttgg atgtttttcg
    10561 tcttgcccct gtgggaagtg ggcagttgaa gaagtggggc ttagtgcttt ctagcattcc
    10621 agctctgatc cgactgcgcg atcagtatga tctgatattt gtgtcgggtt atcggattat
    10681 cggactgaca gctgtactga ttggaaagct gtttcgaaag ccaatcgttc tcaaggcaga
    10741 cagccagggt gagatgtctg gtgaattttt cgaaagtggg cttaggaatt ttaggatctc
    10801 tcgctcatcg ttagtctttc gattatttct gaaaattcgc aatgccattt tgaaaaaagc
    10861 agatgcattt tcagctatct cacctgacat cgctgctgaa tggacatcta gtggagtatc
    10921 acttcacaag attcgcctaa tcccaaacgg tgtggatact gattgttttg ttcccgcaag
    10981 cgcggagcag aaaacgattc tgcgacagaa gctcagcctg cccgaaaccg ctctcatagc
    11041 catttatacc gggcgactcg tttcctacaa agggttgcca ctgctcctaa aagcatggaa
    11101 tgagattcga cgtaagcatc aaaacgccct tctgatcttg gctggtacag gtggcttgga
    11161 tatacacaac tgtgaagatg cactcagaga atatgtgagg gtcaatcggc tcgaacagta
    11221 tgtgctattc acaggtgcag tgcaaaatgt ggccgagtac ctgcaagcct ccgacctttt
    11281 tgcttttccc accgaggacg atgcttttcc atcctctgtt gtagaagcga tggcgtgtgg
    11341 tttgcccgtt gtcacaacgc ccgtgggagc gataaagcat attgttaaag acggcgatac
    11401 aggagttctg gtccagccct ccaatttcac tcagcttttt gaggcattgg atatcatact
    11461 atccaataag gaaataggct cccgcctggc gaaaaccgcc tctcggtctg ttcaggatct
    11521 gtattcggct gaaattgttt caaagaacta tctgacatta tttcagggtc ttattgaccc
    11581 taccaaagcg tgaattccat gagaaacctt cttagaaaaa taccctggct cgcgttcttc
    11641 tttctgttcc tcgggttttt gttcggcgtg attgtcggga tcaaacgggg ggtgcctttt
    11701 gtcgcccagc gtgaacagtg gacgattggc atctaccgtg gtaattcccc ctttcatttc
    11761 aatgaattcc agggatggat aaaccccttg tttcgagcgg aggatatcac cgacgtttcc
    11821 gccaaatttg ttgcggaccc gtttctgatt aaagagaagg aaacttggaa tctctttttt
    11881 gaggtgtatg ataacgacac ccaacagggt gatctggcgg ttgctaccag caataatact
    11941 tggaagtgga attatgaaaa ggtgatactt gatgagccgt tccatttatc atacccctat
    12001 gtgtttaaat ctgaagatag atattatttg ataccggaga gttttgaaga taattcaatc
    12061 cgactgtata aagcagatga atttcctacc aaatggtcat ttgtcaaaac gcttgtgtca
    12121 ggcagggact atgtagacaa ctccatcgtc ttctacaaca ataaatggtg gttgttctct
    12181 tccgtcacca gcaacgataa actctacctc cattatgccg actctctgga aggcaagtgg
    12241 aaggagcatc cccaaagccc gattgtggta ggcgatgttc acaaatctag acccagtggc
    12301 agactgcttg tttacgatgg caggcttttt cgctttacca tggatatcga tccaccagta
    12361 ggcacgcatc aggtgatggt gtatgagatc acagaaatca caagcacgag ctac
//
LOCUS       DASTJP010000492         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_978482, whole genome shotgun sequence.
ACCESSION   DASTJP010000492 DASTJP010000000
VERSION     DASTJP010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978482"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFQ23_14320"
     CDS             <1..279
                     /locus_tag="VFQ23_14320"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET9907822.1"
                     /translation="DPTVQYALGYDILAQTWWKNPLSLQDLQFNSPYNTYIYVGLPPS
                     PIANPSLESLRAVAFPAETTYYFFRAKCDGSGFHLFAVSFEEHLANAC"
     gene            401..1408
                     /locus_tag="VFQ23_14325"
     CDS             401..1408
                     /locus_tag="VFQ23_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HET9907823.1"
                     /translation="MSHFPSLEYSAALIGWGSDVLGYDDAQSTDHNWGPRFQLFLAQE
                     GYEQVQAALHSVLSRKLPSEYLGQPVRFPIVGGEHQQKKAGEASHNIEIQTIKSYFGS
                     YLGCDPYREMEVADWLTFPEHKLLGVTSGEVFHDGLNELELIRQKFRYYPRDVWLYLL
                     AAQWEKISEEQAFVGRCGHVGDELGSALIAARQIKNLMRLCFLMERKYAPYSKWFGTA
                     FNRLACADELSPIFVKVIQAREWMTRQEFLAKAYEVVARMHNALRITVPLQETAAPYF
                     GRPYLVIGDERYTAELRKLVTNEEVRKIAHNLGSVNQFIDSTDQINDLKIREKLKEVY
                     A"
     gene            complement(1413..>2373)
                     /locus_tag="VFQ23_14330"
     CDS             complement(1413..>2373)
                     /locus_tag="VFQ23_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907824.1"
                     /translation="LLFFNIERKAFTSATYKKAFENQNLYVRMPAILANAVTTVVAQT
                     PNLPTFVKTLTVTNWETTIVSVLPPQEVKAITDQALDSVFDYINRRTNSVVISVIPLK
                     SRLVGPSGVEVVKQVLRAQPACTPEQLQAIALGFLSGGDILLCSPPEDVMALLTPVIE
                     SQIPALAFAIPDQITLFPEALSGTPADPRVRINNVRLLLLFSPLLPVTFLLAVTLFAV
                     RGLRDWLIWWGLPLSLTGAGSLLVAVLGAPVVGLSVRGFIQARGASFIPPIFLSTLQE
                     TASSVTSEILNPVAIGGLILAVVGVTMTAAALFVVRKTPEIVY"
BASE COUNT          636 a          545 c          599 g          593 t
ORIGIN      
        1 gaccccactg tgcaatacgc gctcggctac gatatccttg cacaaacctg gtggaagaat
       61 ccactgagcc tgcaggattt gcagttcaat tcaccgtata acacatacat ttacgttggg
      121 ctgcctcctt cgccgatagc aaatcccagc ttagagtcgt tacgcgcggt tgccttccca
      181 gctgagacaa cctattactt ttttcgtgct aaatgtgatg gttcgggatt tcatctgttt
      241 gcagtgagct ttgaggagca ccttgcgaat gcttgttagc cattaaccat aaatacagaa
      301 cacaactccg ttaaatttcg ggaggaattt catgcaggcg tttatgaaag gtttaaagtt
      361 taatcatctt ttttatacgg aagtggtgaa acctatatta gtgtctcact ttccaagcct
      421 ggagtattcg gcagcgctta tcggctgggg atcggatgtt ttgggatatg atgatgctca
      481 atcgactgat cataattggg gaccgagatt tcaattattc ctagcccagg agggttatga
      541 gcaagttcag gctgcccttc atagtgtatt gagcagaaag ctgccttcag aatatttagg
      601 tcagcccgtc aggttcccga tcgttggggg agaacatcaa caaaagaaag cgggtgaagc
      661 ctctcacaat atcgaaatac aaaccatcaa atcctatttt ggcagttatt taggttgtga
      721 cccatatcgt gaaatggagg tggcagattg gttgacattc ccggaacata agcttctggg
      781 tgtcacaagt ggtgaggttt tccatgatgg cttgaatgaa ctggaactga ttcgccagaa
      841 atttcgttac tatcccaggg acgtttggct ttatttgctt gccgcacagt gggaaaagat
      901 ttctgaagaa caagcgtttg tggggagatg cggtcatgtg ggcgacgagc tgggttcagc
      961 tttgattgct gcgaggcaga tcaagaactt aatgagactt tgttttctga tggaacgtaa
     1021 atatgcaccc tatagcaaat ggttcgggac tgcattcaat cgtttggctt gcgccgacga
     1081 attaagccca atatttgtga aggtaataca ggcccgggag tggatgacgc ggcaggaatt
     1141 tctcgccaaa gcctatgaag ttgtcgctcg aatgcacaac gctttgcgga tcaccgttcc
     1201 gttacaggaa acagcagccc cttattttgg acgaccctat ctggtgatcg gtgatgaacg
     1261 ctatacggcg gaattaagaa aattagtgac caatgaagaa gtcagaaaaa tagcccacaa
     1321 tctgggctca gtcaatcaat tcatcgactc aactgaccag atcaacgatc taaaaatccg
     1381 cgaaaagttg aaagaagtat atgcctaata aattaataga cgatttcggg cgtttttctt
     1441 acaacgaata acgcggcagc agtcatggtc acacccacca ccgccaggat taatcctcca
     1501 atcgcaaccg gattaagtat ctcgcttgtg accgaactag cagtttcctg aagcgtggag
     1561 agaaagatgg gtggaatgaa gctggctccc cgggcctgga tgaaccctct cacgctcaac
     1621 ccgaccacag gcgcacccag gacagccacc aataaactcc ccgcgccggt tagagaaagg
     1681 ggcagtcccc accaaatgag ccagtcccgt agcccgcgca ccgcaaagag ggtgacagct
     1741 agcaaaaacg tcacaggcag aagtggagaa aacaataata gcaggcgcac attgttaatt
     1801 cttacgcggg gatcggccgg tgtgccgcta agagcttcgg gaaacaaagt aatttgatcg
     1861 ggaatagcaa atgccagtgc tggtatttgt gactcaatca caggagttaa caacgccatc
     1921 acgtcctcag gcggactgca gaggaggatg tcaccgccag ataagaatcc cagcgcgatt
     1981 gcctgcagtt gttctggtgt acacgcgggc tgggcgcgca gtacctgttt cactacctct
     2041 acacccgatg ggccaaccag gcggctcttg agcgggatga ctgagatcac aactgaattt
     2101 gtgcgcctgt tgatgtagtc aaacacggaa tcaagcgcct gatctgtaat ggccttcact
     2161 tcctgtggcg gcaaaacgga tacgatggtc gtttcccagt ttgtcaccgt cagcgtcttt
     2221 acaaatgttg gtagatttgg ggtttgtgca actacagttg tcaccgcatt ggccaggatg
     2281 gccggcatcc tcacatacag attttgattc tcaaaggcct ttttataggt cgctgaagtg
     2341 aaggcctttc tttcgatgtt gaaaaacaaa aga
//
LOCUS       DASTJP010000493         6916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1105994, whole genome shotgun sequence.
ACCESSION   DASTJP010000493 DASTJP010000000
VERSION     DASTJP010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105994"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..713)
                     /locus_tag="VFQ23_14335"
     CDS             complement(207..713)
                     /locus_tag="VFQ23_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907825.1"
                     /translation="MNTTGPVLELGCGLYSTIMLHTICAKQGRRLHTVDHTLSWLEKF
                     TYLKSQSHTFEHLACGSLNVTVYEFMYRLSQHWEKKIDQSWDQVGQGEKWGLVFLDQL
                     PLSRRVKDIKRLRASTDVFVIHNTNMFGNWLSGFWPYLQTFAYTYIFPAFPATAVCSD
                     KIDVRSFF"
     gene            complement(1091..1588)
                     /locus_tag="VFQ23_14340"
     CDS             complement(1091..1588)
                     /locus_tag="VFQ23_14340"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9907826.1"
                     /translation="MNSKLPPHLYLDGLNIPYEARSFPPETEKGAANVARVLGFAERQ
                     AVKTLIFQVDTGERVLVMLGGDQSAISGNLKKAIGSRNIQMASPDVVKEVTGYVIGSI
                     PAFGWQPPNFRSFLEASLINEPILGTGAGQWGEEIMISPENMIRASHAIVVNLTDREK
                     SVFAA"
     gene            complement(1585..2628)
                     /locus_tag="VFQ23_14345"
     CDS             complement(1585..2628)
                     /locus_tag="VFQ23_14345"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HET9907827.1"
                     /translation="MLNFESALNYHPPASWLKITTIDAHTGGEPFRVIIDGFPELKGD
                     TILERRRHAKENYDHLRTALMWEPRGHADMYGCILTPAVTATADFGILFIHNEGFSTM
                     CGHGIIGVVTAVLETGMLPMLSPVTQIKIDSPAGLITAHAHIQNNEVRNVSFQNVPSF
                     VIALDEIIEVPELGKVRYDLAFGGAFYAYVNAADIGLTCTPKDYRHLIEKGMAIKRAI
                     MENRPIRHPFEDDLGFLYGTIFTGPPTQKASYSQNVCIFAEGEVDRSPTGTGVSGRLA
                     ILHRRGEIRVDQPIVIESILESTFTGKVIGETTFGDYPAIIPEVEGQAHLIGKNEMWI
                     DPNDPLREGFILR"
     gene            complement(2640..3059)
                     /locus_tag="VFQ23_14350"
     CDS             complement(2640..3059)
                     /locus_tag="VFQ23_14350"
                     /inference="COORDINATES: protein motif:HMM:NF014091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HET9907828.1"
                     /translation="MDELTIAGKPVISGSAKGIALVSSEPLSFWGGYDWKSGEITDRR
                     HPLSGSIAKGTILAIPFTRGSSTTTAVLLEAIRAETAPAAIITTETDFFFALASVVAD
                     ELYAKPLPLVAISESDFANIQTGNQIDIEDGIITIRR"
     gene            complement(3047..4312)
                     /locus_tag="VFQ23_14355"
     CDS             complement(3047..4312)
                     /locus_tag="VFQ23_14355"
                     /inference="COORDINATES: protein motif:HMM:NF016310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HET9907829.1"
                     /translation="MTLQLTDKDDEMLRGDHGAATKMAMSILVRMAEVSNATELIDIT
                     GAHIDSTVYIGEAGLEFAERLASLGAKVAVPTTLNVSGLDEHHWKEWSVSSDWARQAH
                     RQMVAYQSMGTIPTWTCAPYQTETRPALGQQIAWGESSAIVFANSVLGARTERYPDLF
                     DICCAITGRAPNIGLHRTENRAGQLLLNVKEIVETVQLRDDFFPILGHLIGKVSSNRI
                     PVIEGICIIPSEDQLKALGAAAASSGAVALFHMVGITPEAPNRKAAFQGNSPGETINV
                     DMDMLRNSRRELTHTDEEKLDIVVLGSPHFSLSEFKSLARLVTGKQKNPDVKFLVTSS
                     RAVTQLANQAGFLEPIQDFGAQFTVDTCILTTPMLPPDMKYLMTNSAKFAYYTPGLLE
                     RKITFGSLKDCVNSAIEGRVIRDESVWTN"
     gene            complement(4316..5050)
                     /locus_tag="VFQ23_14360"
     CDS             complement(4316..5050)
                     /locus_tag="VFQ23_14360"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HET9907830.1"
                     /translation="MMKLQSIAIASLCFLLGCAKPTPSAISSQPIGVPTQPGIDVSAT
                     EPINDPNYFRDDFTSTLAEGWQWLNEDSNNWSLSEVPGALKINVDGGQVSDETIRNLL
                     LRAAPAGNFQIETKVTFKPQADFQIAGLIVYESPPNIIQAGHAFCDLPDVCVGEGLYV
                     DYYNNGNFVTPNFAAAYTNSDVYLRLVRQGDTYTFQSSSNGSEWILRGGTVSTMNPLQ
                     IGLVAGQNSVDLVPALFDYFEVRSLP"
     gene            complement(5338..5913)
                     /locus_tag="VFQ23_14365"
     CDS             complement(5338..5913)
                     /locus_tag="VFQ23_14365"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET9907831.1"
                     /translation="MNKNRLEAFSDGVIAILITIMVLELTVPHSTDFAALQELLPVFL
                     SYVLSFVFLGIYWNNHHHLFQVAKHVNGNVLWANLHLLFWLSLIPFATAWMGENNFAQ
                     LPIALYGFVLWMSGLAYYILTRTLIRLHGNDSLLATALGRKVKEIASLVFYTLAIIFS
                     FISPGITLAIYVFVACIWLVPDQRMERVLSR"
     gene            6096..6695
                     /locus_tag="VFQ23_14370"
     CDS             6096..6695
                     /locus_tag="VFQ23_14370"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET9907832.1"
                     /translation="MAFELPKLAYAYDALEPHIDARTMEIHHTKHHNTYVTTLNGAIE
                     KTPELAGKSLEDLLSDLNAVPESVRTVVRNHGGGTYNHNLFWEIMGPNAGGAPSGDLA
                     KAIDSAFGSFDAFKEEFTKSATTRFGSGWAWLVKKGDGLAVVSTANQDNPLSDGATPI
                     LGIDVWEHAYYLNYQNRRPDYISAWWNVVNWDAVATKYK"
BASE COUNT         1802 a         1655 c         1649 g         1810 t
ORIGIN      
        1 tttatacaaa cccctccaat taccgtgcgg cgttcgacga tggcaacatg cttctggagc
       61 tttgctgcct gaatggctgc ccgctgacct gcgggtcctg agccgatcac gagcaggtca
      121 tagtcatatt cgcccatgtg cacctccgat gatatgaaat tatacttctc ttatgctaaa
      181 tcgcagggtg aatatttcta ctgacactaa aaaaatgatc ttacatcaat cttgtcactg
      241 caaacagctg tcgcggggaa tgccgggaag atataggtat atgcaaatgt ttgaagatag
      301 ggccagaatc cggatagcca attgccaaac atattggtgt tgtgtattac gaatacatcg
      361 gtagaggcgc gtagtctttt gatatccttc acccgcctgc tcaaaggcaa ttgatcaaga
      421 aatactagtc cccatttttc accttgcccg acttgatccc aggattgatc gattttcttt
      481 tcccagtgtt gagaaagcct gtacatgaat tcatacactg tcacattcag cgatccacac
      541 gcgagatgct cgaaggtgtg tgactggctt ttcaggtacg tgaatttttc tagccaggac
      601 aaggtgtgat ccacggtatg cagtcgtcga ccttgctttg cacagatggt atgcagcatg
      661 atggtggagt agagtccaca acccagttcc aggaccggac ctgttgtatt catacagcta
      721 attgctaatg gaacactatg agtgcctgta ttactgctac tgagtgacat gatcatatga
      781 tgacattccc tttcctattg tggttttctg gcgggcagcc atattctaat atcaattcca
      841 aaatgatgaa gagaagaacg gaaataccag gatgtcaagt gctgcaggcg ttgggataaa
      901 ttaagttaga gttctgtacc gcgtcgaggc tgccgatagg cgtcgttgtg atttgataac
      961 agaaaaagcg gcgtgagttg gactgattta gaaaagaaaa ttacagacac caaacagtca
     1021 actggacagt ttgagcttgt atacagatta aattaagaac tcaattggtt tgcgtctaaa
     1081 ttatttatcc ctacgcggcg aacactgatt tctctctatc cgttaaattg accacgatcg
     1141 catggcttgc ccggatcata ttctcaggcg aaatcataat ctcttcaccc cattgacctg
     1201 caccagttcc gaggatgggt tcattaatca ggcttgcttc caaaaaggat cggaagttcg
     1261 gtggctgcca gccaaaggcc gggatggagc cgataacata gcctgtgact tccttgacaa
     1321 catccggtga cgccatttgt atattgcgcg aaccaatggc tttcttgaga tttcctgaga
     1381 ttgcactttg atcgccccca agcatcacta agacacgttc tccggtatcc acctgaaaaa
     1441 taagtgtctt cactgcctgg cgttctgcaa agcccaaaac ccgcgccaca tttgccgcac
     1501 ctttttccgt ttccggagga aaactacgcg cttcgtaagg aatgtttaga ccgtccagat
     1561 ataaatgggg agggagcttc gaattcatct caaaataaaa ccttctcgca gcgggtcgtt
     1621 tggatcaatc cacatctcat tttttccaat gaggtgggct tgcccctcaa cttcagggat
     1681 aatggctggg taatcaccaa aggtagtttc tccaatgact tttcctgtaa acgtgctctc
     1741 gagaatactc tcgatgacaa tcggctgatc caccctgatt tctccgcgcc gatgaagaat
     1801 tgccaatcgt ccactcacac ccgtacctgt cggacttcga tccacttcgc cctctgcaaa
     1861 aatacataca ttctgactat atgacgcttt ttgcgttggc ggtcctgtaa aaatggttcc
     1921 atataagaat cccaggtcat cttcaaaagg gtgcctgatg ggtcggtttt ccataatggc
     1981 tcgtttaatt gccattcctt tttcgattag gtgacggtaa tctttcggcg tgcaggttag
     2041 cccgatatcg gccgcgttga cataggcata aaatgcacca ccgaatgcga ggtcatatct
     2101 tacttttccc agttcaggta cttcaatgat ctcatcaaga gcgatgacaa atgatgggac
     2161 gttttggaat gaaacgttcc ttacttcatt gttttggata tgtgcatggg ctgtgattaa
     2221 cccggccggg ctgtcaattt tgatctgcgt gacaggtgaa agcatgggta acatacctgt
     2281 ttccaacact gctgtcacca caccgatgat cccatgcccg cacattgtgc tgaagccttc
     2341 gttgtgaata aacaggattc caaaatccgc agttgcggtg acggctggcg tgagaatgca
     2401 tccatacata tccgcatgcc cgcgcggttc ccacatcaat gcagtgcgca aatggtcata
     2461 attttctttc gcatgccgcc tgcgttcgag aatagtgtcc cccttcaatt ccgggaagcc
     2521 atcgattatc acacggaatg gttcgccacc ggtatgggcg tcaatagtcg ttatcttcag
     2581 ccagctcgca ggtggatgat aattcaatgc agactcaaag ttgagcatat cgtttctctc
     2641 tagcgacgga ttgtaatgat cccatcttca atatcaattt gattgcctgt ttgaatgttt
     2701 gcaaaatccg attctgagat ggcaaccaaa ggcagcggtt tggcgtagag ttcatccgca
     2761 acgaccgatg ccagcgcgaa gaaaaagtcg gtttctgttg tgatgatcgc ggccggtgca
     2821 gtctctgcac ggatggcttc cagcaacaca gcagtggttg tggatgagcc gcgcgtgaat
     2881 ggaatggcga gtattgtgcc ctttgcaatt gacccggaaa gtggatgtct gcggtcggtt
     2941 atttcgccgc ttttccaatc gtacccaccc cagaatgaaa gtggctcgga actaacgagc
     3001 gcaattccct tcgcggaacc cgaaatgacc ggtttgccag cgatcgtcag ttcgtccata
     3061 ctgattcatc tcgtatgact cggccctcaa ttgctgaatt gacacaatcc ttcaagctgc
     3121 caaacgtgat tttccgttca agcagccctg gtgtgtaata agcaaacttt gcagagtttg
     3181 tcattagata tttcatgtcc ggtggcagca tgggcgtggt aagaatacaa gtatccacag
     3241 taaattgcgc accgaaatcc tgtatcggtt cgagaaaacc cgcctgattt gccagctgcg
     3301 ttacggctcg gctggatgta accaaaaatt ttacgtctgg gttcttttgc ttgccggtta
     3361 ctagccgggc aagtgactta aactcagaca gcgaaaaatg cgggctaccc aagactacga
     3421 tatccaattt ttcctcatcg gtatgagtaa gctcgcggcg tgaattacgc agcatgtcca
     3481 tgtctacatt gatcgtttcc ccgggagaat tcccttgaaa cgcggctttc cggttggggg
     3541 cctcgggcgt gatgccgacc atatgaaaga gcgcaaccgc tccggatgag gcagctgccg
     3601 cacctaaggc ttttagttga tcttcactgg gaataatgca gatgccttcg atgacaggaa
     3661 ttcggttcga ggaaacctta ccaatgagat gaccaaggat cgggaaaaaa tcatctcgca
     3721 gttggacagt ttctacaatc tcttttacat tcaacagcaa ctgcccagcg cggttttctg
     3781 tacgatgtaa gccaatattt ggagcgcgcc cggtaatggc acagcaaata tcgaacaggt
     3841 ccggatatcg ttccgtgcgt gcacccagga cggaattggc gaacacgatc gcgctggatt
     3901 caccccaggc aatttgctga cctagtgcag gccgtgtctc ggtttggtag ggcgcacagg
     3961 tccaggttgg aatcgtcccc atactttgat atgcgaccat ctgtcgatgc gcctgtcttg
     4021 cccaatctga tgacaccgac cattctttcc aatgatgttc gtccaatccg cttacgttta
     4081 gcgtggtggg cacggcaact ttcgcaccca ggctcgcgag tcgttcggcg aactcaagcc
     4141 cggcctcacc aatatagacc gtgctgtcaa tatgagcacc agtgatgtcg atcagttcgg
     4201 tggcattgga aacttcggcc atgcgaacaa gaatggacat cgccatctta gtcgcggcgc
     4261 cgtgatctcc gcgcagcatt tcgtcatctt tatctgttaa ttgaagagtc atgggttatg
     4321 gcaaactgcg gacttcaaaa taatcgaata aagcgggaac caggtcaacg gaattttgcc
     4381 cggccaccag tccgatttga agcggattca tggtactaac cgtcccgccg cgcagaatcc
     4441 attcactgcc gtttgaactg ctttgaaacg tatacgtatc gccctgacga accaggcgaa
     4501 gatagacgtc gctgtttgta taagcagcag caaagttggg tgtgacaaaa ttcccgttgt
     4561 tgtaataatc cacatacagt ccttcgccga cacacacatc gggcagatcg caaaacgcat
     4621 gtccggcctg gataatattt ggcggagatt catatacaat taagcctgca atctgaaaat
     4681 cggcttgtgg tttgaaggtg accttggttt cgatttgaaa gtttcctgct ggagccgctc
     4741 gcagcagcag gtttctgata gtctcatcac tgacttggcc gccgtctaca ttaattttga
     4801 gagcacccgg cacctcactg agactccaat tattagagtc ttcattgagc cactgccagc
     4861 cctccgccaa cgtcgacgtg aagtcatcac gaaaatagtt tgggtcatta atcggttctg
     4921 tggcggagac atctatgcca ggctgagtgg gaacaccaat gggttgtgat gaaatagcag
     4981 agggtgttgg ctttgcacat ccgagaagaa agcacaggga tgcaatcgcg atagattgta
     5041 atttcatcat atcactcctt tgcgtgagtg attataagtt atgttgaata agatggggtg
     5101 cagccgctgc gatggaatca aaagcccctg atgagaaatc aggggctcgg tttgaaacct
     5161 gtctttatgg cagtggcgga gtattctctg cgaaatactc gtgactgtca gcatttctca
     5221 ccgcactgaa ccgaactctg cttctgttaa tctctgttct cttacagcgc caaaggtgac
     5281 tgtatcggag caaaacacca attcagcgtc aagccggaat tggttacggc tctgagacta
     5341 ccgggacaat accctctcca tgcgctgatc cgggacaagc catatacagg caacaaatac
     5401 atagattgcc aatgtaatcc cagggctgat aaaagaaaat atgatagcca gggtatagaa
     5461 aacgagtgaa gcaatttcct taacttttcg accaagagcc gttgccagca gcgagtcgtt
     5521 gccgtggagt cgaataagtg tacgcgtgag gatgtaatag gccaaccccg acatccataa
     5581 tacaaaccca tacagggcaa tcggcaattg agcaaaatta ttttctccca tccatgcggt
     5641 ggcgaacgga ataagcgaca accagaaaag tagatgcagg tttgcccata acacgttccc
     5701 attcacatgt tttgccacct ggaacaaatg atgatggtta ttccagtaaa taccgagaaa
     5761 gacgaagctg agcacatagc taagaaatac tggcagcagc tcttgcaatg cagcaaagtc
     5821 tgtgctgtgt ggaaccgtca gctccaatac catgatcgtg atcagaatgg cgatcacacc
     5881 gtcgctgaaa gcttcaagtc tatttttgtt catagagata aggataaccc aagagtgctg
     5941 gtctgtgcaa gagaaatctc cttttggaaa aggagcactt tattgaatat ctttcttgac
     6001 atgcgctcgt cacagtacta caatttttat cgttgttatt cataacaatc tgttgggggc
     6061 agatcaaact catgtaatct taaaggagac ggaaaatggc ttttgaactt ccaaaactgg
     6121 catatgccta tgacgcgctg gaaccccata tcgatgcgcg tacaatggaa atccatcaca
     6181 caaaacatca caatacgtat gttacgacgc tcaatggagc gatcgaaaaa acaccagaac
     6241 tggctggtaa atcacttgag gacctattaa gcgatttgaa tgctgtacct gaaagcgttc
     6301 gcacggtcgt ccgtaatcat ggcggtggga cctacaacca caatttgttc tgggaaatta
     6361 tgggacccaa tgcgggtggc gctcccagcg gggatttggc aaaagcaatt gattcagcat
     6421 ttggttcgtt cgacgcattc aaagaagagt ttacaaaatc agccacgact cgttttggct
     6481 ccggctgggc atggctggtt aagaagggcg atggacttgc ggtggtttcg actgccaacc
     6541 aggacaaccc actttcagat ggagcgaccc cgattcttgg catcgatgtt tgggaacatg
     6601 cttactattt gaactatcag aaccgccggc ccgattacat ctctgcctgg tggaatgtgg
     6661 tcaattggga tgctgttgca acgaagtata aataaacttc attttgcaac gttttagaag
     6721 caagatataa tagtgtaaac tcagtaaaac cacatttcta tctaggagat gttccatgac
     6781 ccatatcatc acaagtttat gcgtacgcga tcgcggttgc attgatgtct gcccggtgga
     6841 gtgtatgatc cccggtcacc caatcgaaga atggccctgg atctacattg atcctgatac
     6901 atgtatcgat tgtgga
//
LOCUS       DASTJP010000494         5808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_954406, whole genome shotgun sequence.
ACCESSION   DASTJP010000494 DASTJP010000000
VERSION     DASTJP010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954406"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..615)
                     /locus_tag="VFQ23_14375"
     CDS             complement(58..615)
                     /locus_tag="VFQ23_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907833.1"
                     /translation="MPTFRIPQVVHLSPEAVARAAAFAEAVIDTVNYRDSNQALKKKI
                     RDDHYISKLGEEAVSTIFKSRNCEVKGPDYDIYAGKQKSWSADLVINALDVAVKTQRR
                     SAGNRYGLSWTFQDSPRRRDPILDLPDAWVCLVVFEDLKPDLEFVVYPLRRIKQLIFE
                     PPRLSKLLGKKQAVYLETLQKKKVI"
     gene            complement(602..1498)
                     /locus_tag="VFQ23_14380"
     CDS             complement(602..1498)
                     /locus_tag="VFQ23_14380"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9907834.1"
                     /translation="MSKIQPAQNRSVLEQIFISPDQPRLRAGWRLLIQTILFFLFGTI
                     VVVIAASLGIEIDSIVFGNILNLIATTASIYIARRWLDKMSFESLGLKLNPQAAVDVL
                     VGIGITVVQMGLIFVAMLGLGWLTFEGFAWEFDPISTVITGVLTFFIGFIIVGWNEEL
                     LSRGYHLQTIASGINLFWGVVISSAVFALLHLANPSASWVSTTGIFFAGIFLAYGYIR
                     TKQLWLPIGLHIGWNFCEGVVFGFPVSGTETYKLTRIEVTGPEIWTGGAFGPEAGLIV
                     LPALFVGVLLIYLYTADRNANI"
     gene            complement(1703..2479)
                     /locus_tag="VFQ23_14385"
     CDS             complement(1703..2479)
                     /locus_tag="VFQ23_14385"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET9907835.1"
                     /translation="MTEYSSAELMIINAARLLRDGDVVFVGVGQPNLACNLAKRTHAP
                     NLVMIYEAGVIGAEPARLPLSIGDPTLVSGALSVVSMYDIFSNYLQRGNVDVGFMGGA
                     QIDKYGNINATVIGGYDHPKVRLPGSGGSQEIAAWANRCYIMTPHQKRRFPEKVEFMT
                     SAGYISGKGDRQKAGLRGGGMLGVVTDIGFMEPDDSREMILTALHPGKTAEQAKENTG
                     WDLKIAATLRTTEPVTEKELKILREELDPTGIYLKGGGGA"
     gene            complement(2476..3342)
                     /locus_tag="VFQ23_14390"
     CDS             complement(2476..3342)
                     /locus_tag="VFQ23_14390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9907836.1"
                     /translation="MVATSLQEQIKSAFVEYGGIGLPLHFLHANGYPFHCYEPLLNLL
                     HRDYHVFGMLLRPLWEGSKPDEVKDWHIFSDDLLRFLDEYNTAPVIGVGHSIGATVTL
                     RAAIREPKKFRALVLIEPVLFPQGRMIAWNFIRAIRLGNRLHPSIPGALKRRRSFDNY
                     DQIFDRYRNRNVFRYMSDENLRLYIEGITRPAVAGGYELVFSPEWEARIYYTGLRDFD
                     IWRDLPKLQVPVLFLRGAETDTFWEQAANLIIRKQPNARMETLDKSTHLLPLEHPNMV
                     FDILQSFLKEVQ"
     gene            complement(3329..4231)
                     /locus_tag="VFQ23_14395"
     CDS             complement(3329..4231)
                     /locus_tag="VFQ23_14395"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET9907837.1"
                     /translation="MSKRIALSEAIARFVNDGDTVYAAGFTHLIPFAAGHEIIRQGKR
                     DLTLSRATPDLIYDMLVAAGCARKVIFSYMGNPGVGSLRIVRAALEQGKLDWEEYSHF
                     GMITRLQAGASGLPFLPMNQTGVTDLEHANPNIKRIPDPYGGKDVIVVPALNPDVAIV
                     HVQRADENGNAHLWGIIGEQKEAAFAAKKVILTAEEIVDEAVIRSDPNRTMIPGIVVS
                     AVCHVPYACHPSYAQGYYDRDNEFYLAWDKISESTDSVKAWLDEWVYAVKDRNEYWEK
                     LGIETHKRLEIPPLLSQPINYGRY"
     gene            complement(4231..4479)
                     /locus_tag="VFQ23_14400"
     CDS             complement(4231..4479)
                     /locus_tag="VFQ23_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907838.1"
                     /translation="MGSELTYRVGTFLLVLGIGLVVFFFLSESSGNPDFNFFCWGTVS
                     LTLAFVFRAQYKKSVDPSGRFSIVKRLMPKSKEDKGKK"
     gene            complement(4481..5245)
                     /locus_tag="VFQ23_14405"
     CDS             complement(4481..5245)
                     /locus_tag="VFQ23_14405"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9907839.1"
                     /translation="MPWDPTQYHKFQAERSAPFFDLLALVEVRPNLNVVDLGCGTGEL
                     TRQLAETLPNSTVTGIDSSPQMLDAARASLHSQPGLHFEQGDQSQLEGNWDLIFSNAA
                     LHWSEYHSELIPRLYERLNRGGQLAVQVPSNHNHISHQIYRETASEEMFKFILNGFQR
                     YAPVLSIDDYARILFKCGAEQIVVFEKVYAHVLEDADAVVEWISGTALVPYFERLGEH
                     KDEFVRVIREKMRAAMPDSPVFYPFRRTFFSARKPG"
     gene            complement(5297..>5808)
                     /locus_tag="VFQ23_14410"
     CDS             complement(5297..>5808)
                     /locus_tag="VFQ23_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9907840.1"
                     /translation="LPFEHRLWQQEMFLPISTIGKVQNLDEAMKIANNVNYGLTAGFY
                     GSPSETDWFFDNIEAGVTYANRPQGATTGAWPGFQPFGGWKGSGSSGKNGGGYYYVQL
                     YMHEQIRTLVKPASANASKSNGKAAAARRTPPKKLPKGKGSPPRRTSTRQATTRTAKK
                     TTKKPARGK"
BASE COUNT         1457 a         1497 c         1394 g         1460 t
ORIGIN      
        1 caaaagaaaa aggtgattta ataaagcctc gtggaccata tggtccacga ggctttatta
       61 aatcaccttt ttcttttgaa gtgtttccaa atacaccgcc tgctttttcc caagcaattt
      121 actcaagcgc gggggctcaa agatcaattg cttgattctt cgcaagggat agaccacaaa
      181 ttcaagatct ggcttcaaat cctcaaagac aaccaggcat acccaggcat ctggcaggtc
      241 gaggattggg tcgcggcgcc tgggagaatc ctgaaaggtc caggaaagac cgtagcgatt
      301 cccagctgag cgacgctgtg ttttcactgc gacatccaga gcattgataa ccagatccgc
      361 agaccacgac ttctgtttcc ccgcgtaaat gtcgtaatcc ggacctttca cttcacagtt
      421 acgtgactta aaaatcgtag aaaccgcttc ttcccccagc ttgctgatgt agtgatcatc
      481 acggattttt ttctttaggg cctggttgct atcccgatag ttaacagtat ctatcactgc
      541 ctcagcaaaa gctgcagcgc gggcaacagc ttccggtgaa agatgaacaa cttgcgggat
      601 cctaaatgtt ggcattccga tcggctgtgt aaagatagat taagagcacc cctacaaaca
      661 aagccggcaa gacgatcaaa cctgcctcag gaccaaacgc gccacccgtc caaatttcgg
      721 ggcctgtgac ttcaattcga gttaacttat aagtctccgt ccctgagacg ggaaatccaa
      781 aaacgacgcc ttcacagaaa ttccagccga tatgaagccc aataggaagc cacaattgtt
      841 tcgttcgaat atacccgtaa gcgaggaaaa taccggcaaa gaaaatgccc gttgtgctga
      901 cccatgaggc gctggggttg gcaaggtgga gcagcgcaaa tactgccgat gaaatgacga
      961 caccccagaa tagatttatt ccactggcga tcgtctgtaa gtgatagccg cgtgagagca
     1021 actcttcgtt ccacccgacg attatgaagc caataaagaa ggtcaacacg ccggtgatga
     1081 cggtactgat cggatcgaac tcccacgcaa aaccttcaaa cgtcagccac cccagcccga
     1141 gcatcgctac aaaaatcagt cccatttgca cgaccgtaat gccgattccc acaaggacat
     1201 ccaccgcagc ttgaggattt agttttaagc cgagactttc aaacgacatc ttgtccaacc
     1261 agcgtcgggc aatataaatg gaagccgttg tcgcaatgag gttgaggata tttccaaaaa
     1321 cgatggaatc aatctcaata ccaagagagg cggcaatcac cactacgatt gtcccgaaga
     1381 gaaaaaatag gattgtttga atcagcaatc gccagcctgc ccgtagacgg ggctgatcgg
     1441 gcgaaataaa tatttgctca agaactgatc ggttttgtgc aggttgaatt ttggacatac
     1501 gccgactcct ttgtcagctg ggattcgaat gctccgggtc tcacgtgaac gcggcttcgt
     1561 tttacttact ttccccatca gtctcactca gcgcgagcag agaatcaagg attcgatata
     1621 ttgtattcac ttttcaaata cccggccggg aaaaactatc aaattgaccc gtggctttat
     1681 catccgaggg gctgaagcga atttacgcgc ctccgccacc tttgagatag attcctgtgg
     1741 ggtcaagttc ctcgcgcaaa atcttgagtt ctttttcggt gacgggctct gtggttctca
     1801 acgtagctgc gattttcaaa tcccagcctg tgttttcttt ggcctgctcg gccgttttac
     1861 ctggatgtaa tgcggtgaga atcatttctc tggaatcatc aggctccatg aagccgatgt
     1921 ctgttacaac tccaagcatc cctccgccgc gcagcccggc tttctgtcgg tctcctttcc
     1981 ctgagatgta acctgctgaa gtcataaact ctaccttctc cggaaagcgt cgtttttgat
     2041 gcggggtcat aatgtaacag cgattggccc aggccgcgat ctcctgcgag ccgcctgaac
     2101 caggcaggcg cactttcgga tggtcatagc caccgatcac ggtcgcgttg atgttgccgt
     2161 atttatcgat ctgcgcgccg cccataaagc ctacatccac gttgccgcgc tggagatagt
     2221 ttgaaaagat atcgtacatg ctaacgacgg aaagtgcgcc gctgacaagc gttgggtcac
     2281 caatggatag tggcaggcgg gctggttccg caccgatcac gccagcttca taaatcatca
     2341 caaggtttgg cgcatgcgtc cgcttcgcga gattacatgc cagatttggt tgccccaccc
     2401 cgacaaagac cacatcgcca tcgcgtaata aacgcgcagc attgataatc atcaactcag
     2461 ctgatgaata ttctgtcatt gcacttcctt caaaaaagat tgcagaatgt cgaaaaccat
     2521 gttcggatgt tccagtggca ggagatgcgt ggatttgtcc aacgtctcca ttcgagcgtt
     2581 tggttgtttc cgtataatga gatttgcagc ctgttcccag aacgtatctg tttccgcacc
     2641 gcgcaaaaac aggacgggca cctggagttt cggaagatcg cgccagatgt caaaatcgcg
     2701 cagaccggtg taataaatac gcgcttccca ttcgggcgag aacaccaatt cgtagccacc
     2761 agcgacagct ggtctggtga ttccttcgat atacagcctc aaattttcat cactcatata
     2821 ccgaaacaca tttcgatttc tataacggtc aaagatttga tcataattgt caaatgatcg
     2881 tcgtcgtttc aacgcgccag ggatactcgg gtgtagtctg tttcctaacc tgatcgcgcg
     2941 gatgaaattc caggcgatca ttcgcccttg cgggaaaagc accggttcaa tcaataccag
     3001 agctcgaaac tttttgggct ctcgtattgc ggctcgcaat gtaacagtcg ccccgatgga
     3061 gtgcccaacc ccgatcacag gagcggtatt gtactcatca agaaatcgta acaagtcatc
     3121 tgagaatatg tgccagtctt ttacttcatc aggcttggaa ccttcccata agggccgaag
     3181 cagcattccg aaaacatgat agtctcgatg taagagattt agaaggggtt cataacagtg
     3241 aaaaggatat ccgttagcgt gcaggaaatg cagcggtagc ccaataccgc catattcaac
     3301 gaaggcactt ttaatctgct cctggaggct agtagcgacc ataattaatt ggctgactta
     3361 atagcggtgg aatttccagt cgtttgtggg tttcaattcc caatttttcc caatactcgt
     3421 ttcgatcttt caccgcatac acccattcgt caagccacgc tttgactgag tctgttgatt
     3481 cgctgatttt gtcccaggcc aggtaaaact cattgtcacg atcataatat ccctgtgcat
     3541 aagagggatg acaggcatag ggcacatggc aaactgcact gacaacgatg ccgggaatca
     3601 tggttcggtt tgggtcggaa cggatgacgg cttcatctac aatttcctct gccgtaagga
     3661 tgactttttt cgccgcgaac gcagcttcct tttgctcacc aatgattccc cacaaatgag
     3721 catttccgtt ctcatcggcg cgttgcacat gcacgatggc tacgtccggg ttcaacgccg
     3781 ggactacgat gacatccttg ccgccgtatg ggtccgggat gcgtttgatg ttcgggtttg
     3841 catgttccag atctgtgacg cccgtctggt tcatgggaag aaaaggtaaa cctgacgcgc
     3901 cagcctgcag gcgtgtgatc attccaaaat gggaatattc ctcccagtca agttttccct
     3961 gttccagcgc cgcacggaca attcgaagcg agccgacacc cgggttaccc atataggaga
     4021 aaatgacttt tcgtgcacag ccggctgcta ccagcatatc gtaaatcaga tcaggagtgg
     4081 cgcgcgagag cgttaaatct cttttgccct gacgaatgat ttcatgtccg gctgcaaaag
     4141 gaatgagatg tgtgaaccca gccgcatata ccgtatcacc atcgttcaca aatctcgcga
     4201 tggcttctga aagcgcaatg cgtttgctca ctatttcttt cctttgtctt ccttcgattt
     4261 gggcatcagc cttttgacga tgctgaagcg cccgctgggg tctacagatt tcttgtactg
     4321 tgctcgaaaa acaaaagcca gcgtcaggct aaccgtcccc caacaaaaaa aattgaaatc
     4381 gggatttcca gaggattctg ataaaaagaa gaaaaccacc agaccaatcc ctagcaccag
     4441 aagaaacgtt cccacccgat aggtcagttc tgaacccatg tcaacctggc tttctcgcag
     4501 agaaaaaggt tctgcggaac ggataaaaca cagggctatc aggcatggcc gcgcgcatct
     4561 tctcacgtat cacgcgcaca aattcatctt tgtgctcgcc aagtctttca aagtaaggaa
     4621 ctaatgcagt gcctgaaatc cattccacga cggcatcggc atcctccaaa acgtgggcgt
     4681 atactttttc aaagacaacg atttgctctg ctccacactt gaacaggata cgggcataat
     4741 catcaatgga aagtactgga gcatagcgtt gaaaaccgtt caggataaat ttgaacattt
     4801 cttcgctggc agtttctcga tagatttgat gtgaaatatg attgtgattc gaaggaacct
     4861 gtacagccag ctgaccgcct cggttcaatc tctcatacaa tcgcgggatc agttcggaat
     4921 gatattcact ccagtgcaag gcggcattgc tgaagatcag gtcccaattt ccttcaagct
     4981 gagattgatc cccttgttca aagtgcaggc ctggctgcga atgcagcgat gcccgcgcag
     5041 cgtccagcat ttgtggggag ctatcgatgc ctgttacagt gctgttcgga agagtctctg
     5101 cgagttggcg ggtcaattcg ccggtgccac atccgagatc aaccacgttg agattcgggc
     5161 gcacttcgac aagagccaat aggtcgaaga atggcgccga ccgttcggct tgaaacttgt
     5221 gatattgagt tgggtcccag ggcatagttt gaattccctc tcccgcaagg tgtgggagag
     5281 ggcaataatt tttgttttat ttaccgcgtg cgggtttctt tgtggttttc ttggctgttc
     5341 ttgtcgttgc ttgcctcgtg gacgttctcc ttggcgggct gcctttccct ttgggcaatt
     5401 tctttggagg ggttctcctg gccgcggcag ccttgccatt ggatttggaa gcatttgcag
     5461 aggcaggctt aaccagcgtg cggatttgtt catgcatgta gagttgaacg taataatatc
     5521 cgccaccatt cttgccgctc gatcctgaac ctttccagcc tccgaagggc tggaagccag
     5581 gccaggcgcc ggtcgtcgca ccctgtgggc ggtttgcata agtgacgcct gcttcgatat
     5641 tatcgaagaa ccaatcggtc tcgctgggcg atccgtagaa accagcggtg agaccataat
     5701 ttacgttgtt ggcgattttc atcgcctcat ccagattttg aaccttaccg atcgtgctga
     5761 tcggcaggaa catctcctgc tgccacagcc gatgctcaaa cggcaggt
//
LOCUS       DASTJP010000495         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_970216, whole genome shotgun sequence.
ACCESSION   DASTJP010000495 DASTJP010000000
VERSION     DASTJP010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970216"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="VFQ23_14415"
     CDS             complement(<1..1861)
                     /locus_tag="VFQ23_14415"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET9907841.1"
                     /translation="MPSTLPILRARRERRLARQRVSDFRSRNTFLSVGMFISLLLAVC
                     IFAIGFSYANLTRDLPAMETLQGLLNPPDGLLLQPTRIYDRTRENILFTFSPTDSSRR
                     YIPISSTNPQYLPKSLQNAVIATSDPNFWEHAGFTFAEITNYDLHPTIAQRLVFELLL
                     FNEPPSFRRALRERILAAQITAIYGRTQILEWYLNSAHFGRYAFGADAAAQLYFGKSA
                     TELTTSESAILAAVSDSPSLNPYDAPQTALERGRETISLMKIQGLLSEEASANALDES
                     PFFQTPPPPTPQPAAAFTNFLLSQLDSQFSRTRIERGGLTIISTLDFDTQRQASCVTA
                     FYAARLAGLPDPIIECQSLRFLPALPPGLTITDSSTSAVITDPRTGQILAVVGETFQA
                     QEAPLITAHRPGSVLDTFTYLTGFTRGLSPASLIWDIPGRTDVQNFDGEYHGPIRLRV
                     ALANDYPAPAAQILAQMGIENVDKITQSFGLSRDDEVQLSLVHAAGAYGVFAQQGVYF
                     GHQVHETFGPVTILRVEGNDGSVWLDWGTPQAKPVVTPGLAFLMNHALSDESARLNPL
                     RSSGVLEVGRIAAVKLGQTDDRLDAWAIGYSPSRVAVVWTGARQEGALSPRVPA"
     gene            complement(1905..>2122)
                     /locus_tag="VFQ23_14420"
     CDS             complement(1905..>2122)
                     /locus_tag="VFQ23_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907842.1"
                     /translation="IFTGSFNFPIIRMLVLQFSVSAQDFWQAAPDLLILTLDLFIRIA
                     IIYFPLKALEQILRILMEMEFNSRKAT"
BASE COUNT          557 a          533 c          575 g          457 t
ORIGIN      
        1 cggcgggcac gcggggcgat aacgctcctt cctgccgcgc gccggtccac accacagcta
       61 ctctggacgg cgaataacca attgcccatg catcaagccg atcatcggtt tgacccaact
      121 tgacagctgc aattctccca acctccaaca caccggaact tcgaagcgga tttagtcgcg
      181 cagactcatc actcaatgca tggttcatta aaaatgccag cccgggcgtc actaccggtt
      241 tcgcctgcgg ggttccccaa tccagccaca cacttccatc attcccttct actcttaata
      301 ttgtgacggg tccaaatgtc tcatgcacct gatgcccaaa gtagacaccc tgttgagcaa
      361 acaccccgta cgcgccggca gcatgcacca gcgataattg aacctcatcg tcacgcgaca
      421 gaccgaatga ctgagtaatc ttatccacat tctcaatgcc catctgtgca aggatctgag
      481 ccgccggtgc ggggtaatca ttcgccagtg ccacccgcag gcgaattgga ccgtgatact
      541 ccccatcaaa gttctggaca tctgtcctgc cggggatgtc ccatatcagt gacgcgggac
      601 tcagtccacg agtgaagcct gtcagatagg tgaacgtatc cagcacggac cctggccgat
      661 gtgccgttat cagcggcgct tcctgcgcct ggaatgtttc accgacaaca gcgaggattt
      721 gccccgttct tggatcggta ataactgcgc ttgttgatga atcggttatc gtgagccctg
      781 gcggtaaagc tgggagaaac ctcaacgact gacattcaat gatagggtca ggtaagccgg
      841 ccaggcgcgc agcataaaat gcggtgacac aggacgcctg cctttgagta tcaaaatcca
      901 gcgtggagat gatggtcaat ccgccgcgct cgattcgggt gcgcgagaac tgagaatcca
      961 attgagataa aagaaagttt gtgaatgccg cggcaggctg aggcgtaggc ggaggtgggg
     1021 tctggaaaaa cggagattca tccaacgcat tggcactcgc ctcctccgaa agcaatcctt
     1081 gaatcttcat caaactgatt gtttcgcgtc cgcgttcgag cgcggtctga ggggcatcgt
     1141 atggattcag actcggtgag tcgctcaccg cagccaggat cgcagattcg ctcgttgtaa
     1201 gttcagtggc cgatttaccg aagtaaagtt gtgccgcagc atccgcgccg aaggcgtaac
     1261 gaccaaaatg agccgagttg agataccact caaggatttg agtacgtcca tatatggctg
     1321 tgatctgagc cgcgaggatg cgttctctca aggcgcggcg aaaggaagga ggttcgttga
     1381 agagcagaag ctcaaaaacc agacgctgtg caattgtggg gtgcaaatcg tagttggtaa
     1441 tctctgcaaa tgtaaatccg gcgtgctccc aaaagtttgg gtcagaggtc gcgatcactg
     1501 cattctgaag agactttggc agatattgag gattcgtact actgattgga atatatcgac
     1561 gtgatgagtc agtcggggaa aacgtaaaca aaatattttc ccgagtgcgg tcatagatgc
     1621 gggtaggttg aaggagcagc ccatcgggag gattcaataa gccctgcaaa gtttccatcg
     1681 ccggcaggtc acgagtgaga ttggcgtatg aaaagccaat tgcgaagata caaacagcaa
     1741 gaagaagaga gatgaacatc cccacgctca ggaaagtatt ccgtgagcga aaatcactga
     1801 cccgctgcct ggctagccgc cgttcacgcc gcgcacgcag gatagggaga gtagaaggca
     1861 tgaggataag tatacacgcg ggagaggcgg aagaattgaa aagcttatgt ggcttttcgg
     1921 ctgttaaatt ccatttccat caggattcgc aggatttgct ccagggcttt gagagggaag
     1981 taaattatcg cgattcgaat aaagagatcc agggtaagta taagtaaatc tggcgcggct
     2041 tgccagaagt cctgagcact tacagaaaac tgcaatacta gcattcttat aatcggaaag
     2101 ttaaacgagc cagtgaagat at
//
LOCUS       DASTJP010000496         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_730598, whole genome shotgun sequence.
ACCESSION   DASTJP010000496 DASTJP010000000
VERSION     DASTJP010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730598"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFQ23_14425"
     CDS             <1..467
                     /locus_tag="VFQ23_14425"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9907843.1"
                     /translation="GPIALRSVKNPRYCTGCHFFTTQFPGGAEIELAMLFVDVRGSTT
                     MAETMNNTEFSHLMNRFYEATINVLVQADAFIDKLVGDEVTALFLPGYAGKDHARKAV
                     QAGRNYYAPQAMGSRAVHGCPLVSGYIPARRGWDPLQGRAGVPPISPRWATM"
     gene            923..1324
                     /locus_tag="VFQ23_14430"
     CDS             923..1324
                     /locus_tag="VFQ23_14430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9907844.1"
                     /translation="MNKSDRRVKGLGEVSICVRDLDAMHKFYEEVVGLQVLRRDESFV
                     FFKVAEGYGGHSQNLALFEASKLNPQESTLHHIALNIALEDFESEKIRLEGLGLEVNA
                     TEHEWLHVRSLYFYDPEGNHMEFVAYDESVR"
     gene            1614..2090
                     /locus_tag="VFQ23_14435"
     CDS             1614..2090
                     /locus_tag="VFQ23_14435"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9907845.1"
                     /translation="MDIVVNGKKYPVDETPDRILLWVLRDELGLTGTKFGCGAGICGS
                     CTVHVDGEAVRSCLTTISSVAGKEIRTLEGLASENPDGTLQLHHVQEAFIELQVHQCG
                     YCMSGQIMQAVAFLERNPNPTEEEIIAGMDQNLCRCGAYPRIRKAVSRAAEIAAEG"
     gene            2092..>2632
                     /locus_tag="VFQ23_14440"
     CDS             2092..>2632
                     /locus_tag="VFQ23_14440"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9907846.1"
                     /translation="MTETSNNENMKGGRRKLSRRDFLMVVGAAGGLLVGVKLGVLPFA
                     QLKLAEYLDSAGGPPSNIDAAPLAWFEIGTDNQVKIYIPKVEMGQGIHTALAQAAADE
                     LDVPWEFISVLNVGTGKGLDDPVGTAASNSTSSLFPIVRQAGATVREMLRSEGAKQLN
                     VNLNDAIAENGMVALKNDAT"
BASE COUNT          708 a          603 c          754 g          567 t
ORIGIN      
        1 gtggacctat cgctctgcgc tcggttaaga acccccggta ttgcactggc tgtcattttt
       61 tcacaaccca attccccggc ggcgcggaga tcgaattggc catgttgttc gtggatgtgc
      121 gcggctcgac caccatggca gaaaccatga acaataccga attcagtcat ttgatgaatc
      181 gattctacga agccacgatc aatgttctcg tacaggcgga tgcgttcatt gataaattag
      241 tcggggatga agtcactgct ttgttcctcc caggttatgc aggcaaggat catgcacgca
      301 aggcagtgca agccggcagg aattattacg cgccacaggc tatggggagc cgggcggttc
      361 atgggtgccc attggtgtcg gggtacatac cggcgcggcg tgggtgggat ccattgcagg
      421 ggcgagcggg agtgccgccg atttcaccgc gctgggcgac aatgtaaaca tcgctgcacg
      481 ccttgcttca aaagcaggag caggagaagt tctagtcagt gacgcaacat acactgcatc
      541 taatttggat ttgggcaggt tggaacatcg tcaacttgaa ttgaaaggga aaagttcatc
      601 aatcggagtt catgtcctga atggcgacta ggcgacagaa aaagccgatg gttagacaca
      661 gcccccttgc ggggatcaaa cattaacttg gaaattcaac agccccatca accgacaagc
      721 ccacttccca tttgggaagg tcgcgaagca cgccgaaggc gacgggggga taggtccaac
      781 ggacggacac gccgatcaat cgtcacaatc ggggtgaggt ccaaatcaaa aaaggacgga
      841 taagaaaaat tattaggtta tataattcga tacgttgaag agtgcatata tccagatagg
      901 ccatcgcacc aaaggagtag ccatgaataa atcagatcgc cgtgtcaaag gactgggaga
      961 agtttccatc tgcgtcaggg acctggatgc catgcataag ttttacgaag aagtggtggg
     1021 actccaagta ctgaggcggg acgagagttt tgtgttcttc aaagtcgctg agggctatgg
     1081 cggtcatagc caaaatcttg ccctctttga agcgtctaag ctgaatccac aagagtcgac
     1141 gctccaccac attgcgctca atatagcgct ggaagatttt gaatctgaga aaatacgact
     1201 tgagggcttg gggttggaag tcaatgcgac cgaacacgag tggttgcatg tccgctcgct
     1261 ttatttctac gatcctgagg ggaatcatat ggagtttgtt gcgtatgatg agagtgtgag
     1321 ataagtaatt gacaccgaga acatttcatc accccccttt cctctctact cccaaaaggg
     1381 gacaccaatg cttcttgaaa caaaaccttg acttggaaaa tcagcaaaac catcaaggct
     1441 agcgcccttc cttcaggttt tatgtgagta agaacgtcca agcctgatga cgtgggaggt
     1501 tttctaataa ggtaaagcag acctgagaga agacaatgtt ccaatgcaga tgaaatgagg
     1561 ggaaaaccca ccgtagtttt acaggatagc aatttatatt tggagaaaca gaaatggata
     1621 tagtagtcaa cggtaaaaaa tatccagtgg acgaaacgcc tgacagaata ttgttgtggg
     1681 tcttgcgcga tgaactaggg ttaaccggaa caaaattcgg ctgcggcgca gggatctgcg
     1741 gatcgtgtac ggttcatgta gacggtgaag ccgtacgctc gtgcctaaca acgatttcat
     1801 cggtcgcggg caaggaaatt cgcacactgg aagggctggc aagtgaaaac ccagatggga
     1861 cgctgcaact ccatcacgtg caggaggcat tcatcgagtt gcaagtgcat caatgcggct
     1921 actgtatgag tggacagatc atgcaggcgg tcgcatttct ggaaagaaac cccaacccga
     1981 cagaagaaga gatcattgcg ggcatggatc aaaacctttg ccggtgcgga gcgtatcctc
     2041 gcattcggaa agcagtgtcg cgcgctgcag aaatcgcggc ggagggttga tatgactgaa
     2101 acctctaaca atgagaatat gaaaggcgga cgccgaaaac tcagccgccg tgatttctta
     2161 atggtcgtcg gcgcggcagg gggattgctg gttggggtca aattgggcgt gttgccattc
     2221 gcgcaactta aactagccga gtaccttgac tctgcgggag gcccgccgtc gaacattgac
     2281 gcagccccgc tggcatggtt cgagatcggc acggataatc aggtgaagat ttacattcca
     2341 aaagtggaga tgggacaagg gattcatacg gcgctggcac aggcggctgc agatgagttg
     2401 gatgtgccct gggaattcat cagtgtattg aacgtcggca ccggcaaggg cttagacgac
     2461 cccgtgggaa ccgcggcgag caattcgact tcctcgctct ttccgatcgt caggcaggcg
     2521 ggagcgactg tccgcgagat gctacgcagc gaaggcgcga aacagctcaa tgtgaatctg
     2581 aacgacgcca tcgcggagaa tggaatggtc gcgctgaaga atgacgcaac gg
//
LOCUS       DASTJP010000497         5339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_616328, whole genome shotgun sequence.
ACCESSION   DASTJP010000497 DASTJP010000000
VERSION     DASTJP010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616328"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VFQ23_14445"
     CDS             complement(<1..1155)
                     /locus_tag="VFQ23_14445"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9907847.1"
                     /translation="MPLTLEESLAYEFARHWRDENYRPDLHNIDWTRFARLLTHNRMA
                     VLAAPILERVNAQIPPEAQKLLREQKEKYKRSAASLGSALVTYLKAAKTREIPTIILK
                     GLWLCEKIYDNSSMRPGGDIDIIVPKNKVEACLALLNEQGIGEYWPNLLKDEYFNKHH
                     LHQQRSSPDLSIWFEIHWALDHPYTLLTVDYESIFERSKPAQLLGTPIEEMSTPDLLL
                     SLAIHLVKHAIYLPSLFDREDLPRIILADGMLMYYLDVAEVLKKSRDVDWNLTIQLAN
                     QWGAVDILGSVLQVCKKYFDAPIPQEVIAALPVRGPWQVTRRLMARAAEQELGTYEGK
                     PVNRFWQLMLASNGAFILRPVRLLETASYFFPPADFLKRLYGKANPAIRIF"
     gene            complement(1307..2197)
                     /locus_tag="VFQ23_14450"
     CDS             complement(1307..2197)
                     /locus_tag="VFQ23_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9907848.1"
                     /translation="MTTQVASTPPRLFSTRRVLQQQGRTRHLLVLSSWIGMVMLIILL
                     GLAVFPDAVAPYDSKERVALPLQRPTAENILGTNDIGQDLFSELIAGTRASLFTGLIV
                     SFLSVLFGTLVGLLSGYLGGWTDSLLMRLTDLILVLPFLPLVILLSVYLGPNQRNVIL
                     VLALFFWAAPARLIRAQVLRLREDTYIEAARALGANSLQIMSGHLWSGVRPLVLAQIV
                     LVASAAILAESSLSFLGLGDPSGKSWGTMLYFARASGAFLSDAWQWWVLPTGLMITLT
                     VLSLGLIGYSLEQRISNSVS"
     gene            complement(2194..3162)
                     /locus_tag="VFQ23_14455"
     CDS             complement(2194..3162)
                     /locus_tag="VFQ23_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9907849.1"
                     /translation="MTRRFIEFGITFLLAISINFFLPRLMPGDPLKLIAGSAAPQLGQ
                     ERVDALRAQYGLDKPLPEQYVIYLQQLARGDLGTSFRYSGNKTVVEVLTENFRWTMLI
                     VLISLTLALLIGSTLGIWSATRRGDPLDLGMLSGIYVLRSIPPFWIAMLLIPILAIQW
                     QLFPVGDSYSIPRREGWENVKDVARHAVMPVIVLTMAYIPTAFSIMRASMFGIMESDY
                     IRTARAKGVRNVWVVLKHALRNALLPVLTSFMIDLGQLLGGVTVIEAVFNYRGIGNMM
                     FEAVKSRDYPLLQGGFLLFTVTILILNIITEFIYPYLDPRLRQASA"
     gene            complement(3217..4239)
                     /locus_tag="VFQ23_14460"
     CDS             complement(3217..4239)
                     /locus_tag="VFQ23_14460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9907850.1"
                     /translation="MQIFEPTLDLLRQVFGNSIQNCQAELIKVALGWSRVYRVTLFQQ
                     GHSRHESLVVKTIDPNGPASVLEAERELRFYQAIHPDLPIPKPKIYYLATDEATGFHV
                     IIMEDLAETHSIPTHPYQWSRAELESVLLAYAKLHTSKIESLNYPWLAPRHEKDLDLD
                     SIAEQAEIVQRAGVWGDLPNLGEVVEYARESIEKYADAQVTLIHGDTTPANALLPQDL
                     TQPAVLIDWQDVGTGMPEFDLAYLDLQPFDSARLVPRTNVLSLYWEFRARIDPDVPSL
                     EERRARQLHADLVNTLWLVRSASRVALHPYKEGTYPHMHWASQFGIVYNRLRLLIQEI
                     NTVSTL"
     gene            complement(4248..>5339)
                     /locus_tag="VFQ23_14465"
     CDS             complement(4248..>5339)
                     /locus_tag="VFQ23_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9907851.1"
                     /translation="VSGELSQFQGMVGTGAYKLVEVEPTQFYRFEANPDYFRGEPVVP
                     EIIAKIVKDRTQQFNQLRAEEASAVLSSVPPAFVADLESNPDIELAKGSDFFNYIFYT
                     NGSREPFSDPAVRQAIAKAIDTKTLVEVVLLGQGVELPLNWYHPDLAWSINIPHVYDP
                     EAAAAELEAAGLVDSDGDGIRELNGANTDFGILCDVNNPIEVRATELIAGFLNEVGLG
                     SHQNCLDIDSSVALIWPNFVAVAAPDYDMGIWGWSSGPQFQRGFIKFLTNCEFGTVGW
                     GNLTGTCDPTLDERVAALVSNTDASQTEGLSTALQERFAENLPFIPLMSPGGNFAYRP
                     AAYDGWVYMKGTGIMTIWSFLPEGAQDIP"
BASE COUNT         1327 a         1381 c         1456 g         1175 t
ORIGIN      
        1 aaatattcgt atggcgggat tcgctttgcc ataaagcctc tttaagaagt ccgccggcgg
       61 gaagaagtaa gaggcggttt ccaggagtcg aacaggacgc aggatgaatg ctccgtttga
      121 tgccagcatc aactgccaga agcgattgac cggtttgcct tcataggtcc ccagttcttg
      181 ctctgccgcg cgcgccatca gcctacgcgt gacctgccag ggtccacgca cggggagtgc
      241 agcgatcacc tcctgaggga tgggcgcgtc gaaatatttt ttgcacactt gaagtacaga
      301 gcccaggata tccaccgcac cccattggtt cgcaagttga atcgtcaaat tccaatccac
      361 atcccgagat ttcttcaaaa cctcggcaac atccaggtaa tacatcagca tgccgtccgc
      421 gaggataatg cgcggcaggt cctcacggtc aaacaagctt ggcagataga tggcatgctt
      481 gaccagatga atcgccaggc ttagcaatag atctggggtt gacatttctt cgatgggtgt
      541 gccaagtagc tgtgcaggct tcgaacgttc gaagatactt tcgtaatcga cggtaagcag
      601 cgtatacgga tgatccaacg cccagtgaat ctcgaaccag atgctgagat cggggctgga
      661 acgctgctgg tgaagatgat gtttgttgaa atattcatcc ttgagcaaat tgggccagta
      721 ttcacctatg ccctgttcat tcaacaaggc gagacaggcc tcaactttgt ttttcggcac
      781 aatgatgtca atatcgccac cgggacgcat ggaggagttg tcatagattt tttcgcacag
      841 ccacaagcct ttgaggataa tggtcggaat ctcgcgagtt ttcgcggctt tgagataagt
      901 gacgagtgcg cttcccaaac tggcggcgga gcgtttgtat ttctcttttt gctcgcgcag
      961 tagtttttgt gcttcgggtg gaatctgcgc gttcacgcgc tccaggatcg gagcggcaag
     1021 cactgccatg cgattgtgag tgagaagacg cgcgaaacga gtccagtcga tgttgtgcaa
     1081 atcgggacga taattttcat cgcgccagtg acgggcgaat tcgtaggcga gggattcttc
     1141 gagggtgaga ggcatagttg tttctgattt tatgaacaat cggtggtcga gtagggcgag
     1201 tgattgtgtc gcccgtatcg agaccacaaa gctttacaag gtttccttgt catagaaatt
     1261 acctacaact ggtggtttcg actgctcgcg ctcaaccacc gaactttcat gaaacagaat
     1321 ttgatatcct ttgctcaagg ctatatccga tcaagccgag gcttaatacc gtgagcgtga
     1381 tcatcaagcc agtggggagc acccaccact gccacgcgtc agataaaaag gcgccgcttg
     1441 cgcgagcgaa gtacaacatc gtcccccaac tcttcccgga cgggtcacct aaacctaaaa
     1501 agctcaggct cgattcagcc aggatggccg ccgatgcgac aagcacgatc tgtgccagga
     1561 ccaggggtct gacgcctgac cagagatgtc cgctcataat ttgtaacgaa ttcgctccca
     1621 atgcgcgcgc ggcttcgatg taggtatctt cacgcagccg caggacttgt gcgcggatca
     1681 agcgcgccgg agccgcccaa aagaacaaag ccagaaccag aatcacattc cgctggttgg
     1741 gacccagata gacactgaga agaatcacga gcggcaaaaa cggcaatacc agaattaggt
     1801 ccgtgagacg catcagcaac gagtcggtcc aaccgccaag gtagccgctg aggagtccaa
     1861 ccagggttcc gaacaatacc gaaagaaacg agacgatcaa accagtaaac aacgaagcgc
     1921 gagtccctgc gatgagttcg ctgaacagat cctgtccaat gtcatttgta ccgaggatat
     1981 tttccgctgt cggtctttga aggggcaaag cgactcgctc ctttgagtca taaggcgcca
     2041 cagcatccgg gaacaccgcg agaccaagca ggatgatcag catgaccata ccgatccaac
     2101 tcgagaggac gagcaggtgc cgagtgcgcc cttgctgttg aaggactcgc cgcgtggaaa
     2161 ataaacgcgg cggagtcgat gcgacctgtg tcgtcatgcc gatgcctgcc gcagacgcgg
     2221 atccagatag ggataaatga attccgtgat gatattgagg atcaaaatgg taacggtgaa
     2281 caacaggaat ccgccttgca acaagggata atcacgtgac ttgacggctt caaacatcat
     2341 attaccaatg cctcgataat tgaacaccgc ttcgatcaca gttaccccgc cgagcagttg
     2401 accgagatcg atcatgaacg aggtcaaaac cggcaacagc gcgtttcgca aggcgtgctt
     2461 taacacgacc cagacattcc ttacaccttt ggcgcgcgcc gtgcgaatgt aatcagattc
     2521 catgatgcca aacatggacg cgcgcatgat tgaaaatgca gttgggatgt aggccatggt
     2581 gagcacgatc accggcatca ccgcgtgacg cgctacatct ttgacatttt cccagccttc
     2641 ccgccgcgga atggaatacg agtcgccgac tgggaaaagc tgccactgaa tcgccagaat
     2701 cggaatcaac aacatcgcaa tccaaaacgg cggaatagag cgcagaacat agatgccgct
     2761 gagcataccc agatccagcg gatcgccgcg ccgcgtcgca gaccagattc caagggtgga
     2821 cccgatcaat agagcgagag tcaagctgat gaggacaatc aacatcgtcc agcgaaagtt
     2881 ttccgtcaag acttcgacga ctgtcttatt tccactatat cgaaaggatg tgccaaggtc
     2941 gccgcgtgcc agttgctgga ggtaaataac atattgttct ggtaaaggct tatcgagtcc
     3001 gtactgtgcg cgcaaggcgt caacacgttc ctgacccaat tggggcgccg cgctgcctgc
     3061 gatcaacttc aacgggtccc cgggcatcag gcgcggcaaa aagaaattga tcgaaatcgc
     3121 gagcaggaag gtaataccaa attcgatgaa gcggcgtgtc atgaaaaaga ggcgtccgaa
     3181 ttgaaatctt cgcgcggagg ccggtccttg ttatggctat agagttgaga cggtgttgat
     3241 ttcctggatt aataatctca agcgattata gacgataccg aattgcgaag cccagtgcat
     3301 atgcggatag gtcccttctt tgtatggatg gagtgcgacg cggctggcgg aacgtaccag
     3361 ccatagcgta ttcaccagat ccgcatgaag ttgccgcgcc cggcgctctt caagcgaagg
     3421 aacgtccggg tcgatcctgg cgcgaaattc ccaatacagc gagagcacgt tggttcgtgg
     3481 aacgaggcgg gctgagtcga agggctgcag atcgagatac gccaggtcaa attcgggcat
     3541 gcccgtgcct acatcctgcc agtcaatgag aacggccggc tgtgtcaagt cctgcgggag
     3601 cagtgcattg gcaggcgtgg tatctccatg gatcagcgtg acttgagcgt ctgcatattt
     3661 ctcaatgctt tcgcgcgcgt actcgaccac ctccccaaga tttggcaagt cgccccaaac
     3721 cccggcgcgt tgaacaatct cagcctgttc cgcgatgctg tccagatcca aatctttttc
     3781 gtgccgcggc gcaagccagg gataattcag cgattcgatt ttacttgtgt gaagttttgc
     3841 gtatgccagc aggacagact ctaattcagc gcgcgaccat tgatatggat gcgtcggaat
     3901 ggaatgagtt tccgccaggt cttccataat gatgacgtga aagcccgtgg cctcatcggt
     3961 agccagataa taaatcttcg gtttgggaat gggcaaatct ggatgaatcg cctgataaaa
     4021 acgcagttcg cgttctgctt ccagcacact ggctgggcca tttggatcta tcgtcttaac
     4081 gacaagggat tcgtgcctcg agtgaccctg ctgaaagagg gtcactcgat agacacgact
     4141 ccaacctaag gcaactttaa ttaattctgc ctgacagttc tggatgctgt ttccaaatac
     4201 ctgtcggagc agatcgagag taggctcgaa gatttgcacg ttggtaacta tggaatatcc
     4261 tgtgcgcctt cggggaggaa ggaccagatc gtcatgatgc cggtgccttt catgtagacc
     4321 cagccatcat aggcggctgg acggtaggca aagtttccac cgggcgacat gaggggaatg
     4381 aatggcaggt tctctgcgaa tcgttcctga agtgctgtcg agagaccttc ggtctgggat
     4441 gcatctgtgt tcgatacaag cgccgcaacg cgttcatcca gggttgggtc gcatgtaccg
     4501 gtcaggttgc cccatccaac ggtgccaaat tcgcaattgg tgaggaattt gatgaacccg
     4561 cgctggaact gcggacctga tgaccagccc cagataccca tatcgtaatc gggtgcagcg
     4621 accgctacaa agttcggcca gatcaacgcc accgacgaat cgatatcaag acaattctga
     4681 tgcgaaccaa gtcccacctc attgaggaag ccggcgatta gttcggtcgc acgcacttca
     4741 atcgggttat tgacgtcaca taggatgcca aagtctgtat tggccccgtt gagttcgcga
     4801 atcccatcgc cgtctgaatc caccagccca gccgcttcga gttcagcagc cgcagcttca
     4861 gggtcataga catgggggat attaattgac caggctaaat cggggtgata ccagttcagt
     4921 ggcagttcaa cgccctgtcc aagcagaaca acctctacca gggttttggt atcaatggct
     4981 ttggcaattg cctgtctcac tgccgggtcg ctgaacggtt cgcgcgagcc gttggtgtag
     5041 aaaatgtaat tgaagaaatc ggaacccttg gcgagttcga tatctggatt gctttccaga
     5101 tccgcgacaa acgcaggtgg cacagaggat agcacagcac tggcttcttc tgcgcgcagt
     5161 tgattgaatt gctgggtgcg gtctttgacg atcttggcga tgatctccgg aacaaccggc
     5221 tcaccgcgga agtaatcggg gttggcttcg aatcggtaga actgggttgg ctcgacctca
     5281 acgagtttat aagcacccgt gccaaccatt ccctgaaact ggctcagctc gccactgac
//
LOCUS       DASTJP010000498         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_238637, whole genome shotgun sequence.
ACCESSION   DASTJP010000498 DASTJP010000000
VERSION     DASTJP010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238637"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="VFQ23_14470"
     CDS             complement(<1..1773)
                     /locus_tag="VFQ23_14470"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET9907852.1"
                     /translation="MGNLPARGRQMPRRIRMIQIILLLLVSIISYGALVLPLALRPTA
                     LPLQPGDVSPRDFQAQEPRDYISEVRTEELRDAAENAVPDVYAPPDPSIPRIQIEKLR
                     AALQYITLVRNDEFATPEQKISDIASLGEVSLNSQTIEKIVGLPDSRWEGLDLEALSV
                     LEQVMRGEIRDDDLQTVERTIPSLVSLALNEDQVGLVAELVTAFVIPNSIYSENLTAT
                     AREAARDAVEPFIVSYKAGETIIPNGDIITPVQFEALQVYGYIDEPNPWQEYAGAGAL
                     VLMLAVFVNLYFSRRKMQFLYDSRSLAVIALIFVLFIVSARAAIPYRTILPYAFPLPA
                     MGLLIATLFGMEVGVIFSLMIALLVPYSLPNTMDLMPYYIISSLTGVMLLGTARRVWT
                     FFRAGMAIALAGIAMLAAFRFPFTETDELGLISLGGAALFNGLASASIALLLQYFLAQ
                     SLGLTTALQLIEISRPDFPLLQLFLRNAPGTYQHSLQVANLAEQAAEQIGADALLTRV
                     GALFHDVGKSTNPMFFIENQPQGQINTHEDMDPTEAAETIIAHVLDGVSLARKYRLPR
                     RIDDFILEHHGTMITRYQYTHALAA"
     gene            complement(1898..2347)
                     /locus_tag="VFQ23_14475"
     CDS             complement(1898..2347)
                     /locus_tag="VFQ23_14475"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET9907853.1"
                     /translation="MTIKNQLNDSMKDAMKSGDEVRKRTVRMVLAAVKQAEVDKRIEL
                     DDAAVMNLIQKEMKNRRESMEEAKKANRADLIEANEAEIKVLEAFLPKAMPAEELRAI
                     VQSIIDETGASTPADMGKVMKAIMPKVAGRAPNDIVSATVRELLQPK"
     gene            complement(2416..3399)
                     /locus_tag="VFQ23_14480"
     CDS             complement(2416..3399)
                     /locus_tag="VFQ23_14480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9907854.1"
                     /translation="MHYRELGRTGWNVSTISFGAWAIGGTWGNVKDDESLSALHRALD
                     LGVNFFDTADVYGDGRSEQLLAKLRKERSEPFYVATKAGRRLSPHTATGYTKENLTAF
                     VESSLKNLEVEAIDLLQLHCPPTQVYYMPEVFDALDGFVKEGKLRYYGVSVEKVEEAL
                     KAIEYPNVQSVQIIFNIFRQRPSDLFFSEAQRRSVGILARLPLSSGMLTGKMDRTSSF
                     EADDHRHFNRQGAGFDRGETFSGLDYEIGLQAVEELRNLLPATMTLTQLALRWILMSP
                     TVTCAIPGAKRTSQVEENISAVDLPALSDETMSKVREIYDRLVRPQVHHYW"
     gene            complement(3550..4425)
                     /locus_tag="VFQ23_14485"
     CDS             complement(3550..4425)
                     /locus_tag="VFQ23_14485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907855.1"
                     /translation="MAELQQDIWSRWLLNRRFGGDPERLTHVLDYLYPVREKVLSHIN
                     LEGGRTLLDIGCGDGLIAFGALEKFEGSRAIFTDISDDLLYHVQSLARHMNLLHRCEF
                     VRASADDLGMFEDESIDAVTTRSVLIYVYAKLQAFKEFHRVLKPGGELSIFEPINRFS
                     YPEPEGSFSGYDVTPISSLAEKLKAVYRRIQPPDTDPMTDFDERDLSICAEKAGFSEI
                     HVELQIEVKPPENSDWAKFLHVPGNPKIPSIEEAMQQTFTLGETETFTSYIRPLVESR
                     HGVHRSAVAYLWAVK"
BASE COUNT         1140 a         1173 c         1161 g         1118 t
ORIGIN      
        1 ggctgccaac gcatgcgtgt attggtagcg tgtgatcatg gtgccgtgat gttcgaggat
       61 aaagtcatca atacgacggg gcaggcgata tttacgtgcc agcgaaaccc catccaaaac
      121 atgggcaatg atcgtttctg cggcttctgt cggatccata tcttcatgcg tattgatctg
      181 tccctggggc tggttttcga taaagaacat gggattcgtg gacttgccca catcgtgaaa
      241 gagggcgccc acgcgtgtga gtagggcgtc cgcgccgatc tgttcggcag cctgttccgc
      301 caggttcgcc acttgcaggc tatgctggta ggttcccggc gcattgcgca aaaagagctg
      361 aagcaaaggg aaatcgggtc gcgagatttc gatcaactgg agggcagtgg tcaaccccaa
      421 actttgcgcc aggaaatatt ggagcagaag agcaatgctt gcggatgcca atccgttgaa
      481 gagtgccgca ccgcccaaac taattaaacc aagctcgtcc gtttcagtga aaggaaaacg
      541 gaacgccgcc agcatggcga ttcccgcgag ggcgattgcc atccccgccc ggaaaaatgt
      601 ccagacgcga cgtgcggtgc ctaacagcat cacaccggtc agggaggaaa taatatagta
      661 gggcatcagg tccattgtgt tcggtaaaga gtatgggaca agcaatgcga tcatcaacga
      721 aaagatgaca cctacctcca tcccgaacaa cgtcgcgatc aacaaaccca tggctgggag
      781 tggaaatgca tagggaagaa tggtgcgata cggaatcgct gcccgggcac ttacgatgaa
      841 cagcacaaag atcaaggcga tcacagccag gctgcgacta tcgtacagga attgcatctt
      901 ccgtcgtgaa aaatacaaat ttacgaaaac tgcgagcatt aataccagcg caccggcacc
      961 ggcatattcc tgccagggat tgggttcatc aatatacccg tagacctgta aagcttcaaa
     1021 ctgaacaggc gtgatgatat ctccgttggg tatgatcgtc tcgcctgctt tgtacgatac
     1081 gataaaaggc tccacggcat cccgggccgc ctcgcgggct gtggctgtca ggttctcgct
     1141 gtaaatgcta tttggaatga cgaacgctgt cacaagctct gccaccagcc ccacctgatc
     1201 ttcattcagg gcgaggctga ccagagaagg aatcgtgcgt tcaacggtct gcagatcatc
     1261 gtcacggatc tcaccgcgca ttacctgttc aagcacactc agcgcttcca ggtcgaggcc
     1321 ttcccagcga ctatctggca gcccaacgat cttttcgatg gtttgtgaat taagcgaaac
     1381 ctcacctaac gaagcaatat ccgatatttt ttgttccggc gtggcaaatt catcgtttcg
     1441 gacaagcgtg atgtattgca gggctgcgcg aagcttctcg atttggatgc gaggaatgga
     1501 tgggtctggt ggcgcgtaaa catccggaac cgcgttctct gccgcgtcgc gcaattcttc
     1561 agttctcacc tcactgatgt aatcgcgtgg ttcctgagcc tgaaaatctc ttggtgaaac
     1621 atctccgggc tgaagaggga gggcagtcgg gcgcagcgcc agtggcagca ccaaagcccc
     1681 atacgatatg atgctcacca aaaggagcaa aatgatttga atcatgcgga tgcgccgagg
     1741 catctgacga ccacgcgcag gcaaattccc catcttagtt caaaagaatt aaccgcagtg
     1801 aaatgccgtc actgcaattc tttaaaagaa tgtccctgtg tcttcgtatt cccgagatgg
     1861 ggcgtgccag agcggcaaaa gatcgtgggg acatgtatta tttgggttga agcagttcgc
     1921 gcacggtggc actgacgata tcattcggtg cgcgccctgc gacctttggc atgatggctt
     1981 tcattacctt ccccatatct gcaggcgtgg acgcgcctgt ttcatcaatg atggattgaa
     2041 caatggcgcg taattcttct gcaggcatgg ccttgggcag gaaggcttcc aggactttga
     2101 tctccgcttc gtttgcttca atcaaatccg cgcgattcgc tttctttgcc tcctccatcg
     2161 actcgcggcg gttcttcatt tccttttgaa tgagattcat tacagccgca tcatcaagtt
     2221 cgatgcgctt atccacctcg gcttgcttga ccgcggccaa caccatgcgc acggtccgtt
     2281 ttcgcacttc gtccccgctt ttcatcgcgt ctttcataga gtcgttcagt tggttcttga
     2341 tagtcatgat aaagaattat actagagaag ggtaggagaa ataaattggt tgattcccca
     2401 gcaggagatt tgggatcacc agtaatgatg cacctgcgga cgaaccaggc gatcataaat
     2461 ctcgcggacc ttgctcatcg tttcatcgga aagtgcaggc agatcgacag cgcttatatt
     2521 ttcttcgact tgtgacgtgc gttttgcccc tggaatggca caggtcaccg tgggcgacat
     2581 caagatccag cgcaatgcca gttgagtcag agtcatagta gcggggagta gattccgcag
     2641 ttcctccaca gcctgcaatc caatttcgta gtccagtccg gaaaacgttt caccgcgatc
     2701 aaaaccagca ccctgacggt tgaagtgacg atggtcatct gcttcaaatg aactcgtgcg
     2761 atccattttg ccggtcaaca tgccagaaga cagcggcaat cgggcaagga ttcctacgct
     2821 tcgtcgctga gcttcgctga agaataaatc cgatgggcgc tggcggaaga tgttgaaaat
     2881 gatctgcacc gattgcacgt tcggatattc aatcgctttg agtgcttctt ctaccttttc
     2941 aacactcaca ccatagtaac gcagcttgcc ctctttgacg aaaccatcca gcgcatcgaa
     3001 tacctctggc atgtaataga cttgtgtggg cggacaatgt aactgcagaa gatcaatggc
     3061 ctccacttca aggtttttca gactgctttc cacgaaggcg gtcaggttct ctttggtata
     3121 tcccgtggca gtatgtggac ttaatctgcg gccggctttg gtcgctacat aaaatggttc
     3181 actgcgttct ttgcggagtt ttgcgagtag ttgttcactc cgcccatccc catagacatc
     3241 cgccgtatcg aagaaattca cgcccaggtc gagggcgcgg tgtaatgcag aaagggactc
     3301 gtcatctttt acattccccc acgtcccgcc gattgcccaa gcgccaaaac taatggttga
     3361 gacgttccaa cctgtgcgtc caagttcgcg ataatgcata ctacctctaa tgatatatgt
     3421 aaagataagc tgattaataa taacagttaa ccggcgcttt cgagaatagt caaccaaagc
     3481 cgccagttaa cgaataaaag atatttgatt tatcttatgt tagtcccacc tgatgcattt
     3541 tctactttac tacttcacag cccacaagta agccaccgca gagcgatgga cgccgtgtct
     3601 tgattccacc agcgggcgaa tatagctggt aaatgtctct gtttctccca gggtgaaagt
     3661 ctgttgcatt gcttcttcaa tgctgggtat tttggggtta ccgggaacat gcagaaactt
     3721 tgcccaatct gaattttcag gtggcttgac ctcgatctgg agttcaacgt ggatttctga
     3781 aaacccggcc ttttctgcgc agatggacag atcacgttcg tcaaaatctg tcatcgggtc
     3841 agtatctggt ggttggatgc gtcggtagac ggccttgagt ttttccgcaa gggatgaaat
     3901 gggtgtcaca tcatagccgg agaagctgcc ctccggttcg ggataagaga agcggttgat
     3961 cggttcgaag atcgacaact cgccgccggg cttgagcacg cgatgaaatt ccttgaaggc
     4021 ttgcagcttc gcatacacat agatcaatac cgaacgagtc gtaaccgcat caatggattc
     4081 atcctcaaac atgccaaggt catccgcgga ggcacggaca aattcgcaac gatgcaaaag
     4141 attcatgtgc cgcgccaggc tctgaacgtg atatagcaag tcatccgaaa tgtcagtaaa
     4201 aatagccctg gaaccctcga atttttccaa ggcgccaaag gcgatcagcc catcaccgca
     4261 gccgatatcc agcagggtcc ggccaccctc cagattgata tgacttaaca ccttttcccg
     4321 gaccggataa agataatcca gcacgtgcgt caaacgttcg gggtcaccgc caaagcgccg
     4381 attgagcagc cagcggctcc agatatcttg ttgtaattct gccattacat tctccagagg
     4441 tcgttcatgt tatcaattta cagattcaaa agattccata gtaggatgaa tcatgagtgc
     4501 acaagcgcag ttccatccac tggtgtttga aggtattgaa gtgtgttcga aatgtgtact
     4561 tctgaatcgg acttcataaa ctaaaaatgg cc
//
LOCUS       DASTJP010000499         6234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_649293, whole genome shotgun sequence.
ACCESSION   DASTJP010000499 DASTJP010000000
VERSION     DASTJP010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649293"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFQ23_14490"
     CDS             complement(<1..196)
                     /locus_tag="VFQ23_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907856.1"
                     /translation="MPSMIPAIVIPAYNRPDVLSRLLASLALAKFPSDFKVPLVISID
                     LEQDGPNHGVRTVAESFDWQY"
     gene            complement(196..906)
                     /locus_tag="VFQ23_14495"
     CDS             complement(196..906)
                     /locus_tag="VFQ23_14495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907857.1"
                     /translation="MDPELYLRVREKEGRLYSDQIVAGLPSVPDGHPLVSEWRARSES
                     ARRLLRYISSRQSPFFVLDLGCGNGWLSNLLAQPGQVVLGLDQNRYELRQAARVFRSN
                     PNLSFLEADIFSAPFPAGSFDLIIVASALQYFPNATNLLTMLLDYLKPNGEIHILDTP
                     LYSKRERETAVLRSREYYASLGCPEMIDHYFHHRISDLDSLHPKMLYDPGSLTRRLKR
                     WIGQIDSPFPWVVIRKQN"
     gene            complement(896..1696)
                     /locus_tag="VFQ23_14500"
     CDS             complement(896..1696)
                     /locus_tag="VFQ23_14500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907858.1"
                     /translation="MLDLNAVDLGFSRKAEVYDEYCENHPVIRWARDVIRHEAVQRLP
                     GNASILELNAGTGSDAAYFVQQGYRVHATDIASGMVAAIQKKIALLQRADQFTVQKLS
                     FTELDLVEGGPYDLVFSNFGGLNCIPALDGVVNTIPRLLKSGGHVLCVIMPPICPWEL
                     AQVLRGKFRLATRRLHREGVLANVEGAQLMTWYYHPEQVIKAFGHNFRLVKQQSISLF
                     CTPSYMDQFPHRFPSFTRFLLQMDEHFGRRAPFNRWGDFVAYTFQYGP"
     gene            complement(1689..3176)
                     /locus_tag="VFQ23_14505"
     CDS             complement(1689..3176)
                     /locus_tag="VFQ23_14505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9907859.1"
                     /translation="MTHILLGQSYYLRFDPKLWDAMQPYPPLGTLYAASYLRQNGYEV
                     ALFDAMLAESEDEWGTSLENHRPQYAIIYEDNFNYLSKMCLLRMREAAFKMIEMAKRI
                     GCTVILCGADATDHPEEYLRRGADYCLLGEGEETLIELLDQMSGKREVALESIKGLAW
                     PGLQTMDDGPLTIVDRPSSIVQNPRRPDITNLDKLPFPAWDLVDVQAYRSIWMERHGF
                     FSMNMVTTRGCPYHCNWCAKPIWGQRYNSRSPENVAAEMKWIKENFAPDHIWFADDIL
                     GLKPHWLEKFAALLQEMDSVIPFKCLQRADLVNEKTAAALAKAGCKTVWVGAESGSQK
                     ILNAMEKGDTVDDIYEAARLLRSNGIEVGFFLQFGYPGETWEDVQKTLRMVRECAPDD
                     IGISVSYPLPGTKFFERVKLELGEKRNWQDSEDLAMLYRGPFPTEFYRILHGRVHYEF
                     RLRQTILKRDPKRWIRIPYYLIGLLFSEMRLKQYANTSRNLVVNA"
     gene            complement(3184..4101)
                     /locus_tag="VFQ23_14510"
     CDS             complement(3184..4101)
                     /locus_tag="VFQ23_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907860.1"
                     /translation="MLDHSSRDLSRAILQTVAYSDLFDYPLSAREIHRYLTGIKASLE
                     EVVRALREDGVVGQTDSYFTLHGREEIVRIRMQRVFHSSLLLPSAIAYGRMLGNLPYI
                     RMVALTGSLAVMNVSGNPDFDYMLVTTPGRLWIARAFALALNRLARLQGYTLCPNLLV
                     SELALEWPEKDLYSARELCQMIPIAGFDTYRRLLQVNRWIERFLPNAYAELEKTLPAV
                     GKPAGAFQALLEFVLLGNFGSRFEQWEMNRKIARFSKQAGFGEETVFTAEVCQGNFHH
                     HRKWTREMFENKLSVLEKESAVTFKVTVT"
     gene            4180..5367
                     /locus_tag="VFQ23_14515"
     CDS             4180..5367
                     /locus_tag="VFQ23_14515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9907861.1"
                     /translation="MHLTIVSPFPPTITGIGQYGYHVTRSLAKSGSFSRVTVLAGSNG
                     NGEQPNHLGTTELDYCWEPGQLNARRAIVSRIKRLRPELVWFNLGASVFGKSPWTNLS
                     GLFTPMLIQRLGIPTVITLHELAELSDLRALNAPGGPFAHLGARVITGIATRADVTCL
                     TMRNYADWLSVKQITCTHIPIGAYHEPNLLEESDSQELLFFTTLAPFKGLELLLEAFR
                     VLRREFPALRLTIAGTEHARFPNYAHELRTHFNGTEGIHWRGQIAEDHVMELFRKAQI
                     VVLPYTASTGSSSVLYQAATWGRPVVVSDLKEIRALAKESNLLLEFFKNGDLQSLCQS
                     LRALLNSPEKRRRQAWANFTSIQHARPEVTCRKYIEAFNYALRKRGNENRIRIPSTTL
                     ETG"
     gene            5364..>6234
                     /locus_tag="VFQ23_14520"
     CDS             5364..>6234
                     /locus_tag="VFQ23_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907862.1"
                     /translation="MIPRRVKIAILIAFLFHGLFILTGRYRLSYDAYTHILFANHYAE
                     NWFSLWEPRWYTGFTVVSYPPLIHQLIALFIPLLGFEGAFALMLWVVTTLYPIGLYGL
                     SRVFVGKSASSYAALFSAALLPLYVVAHIFGQLPFLASTLIALFGAVSLNRYLREGGI
                     LNLLLTVSLITTSIAAHHATLMIHPFLVFAVLIKNIANHRSINRLQEGRSQIFVTWWF
                     ALFSRLMLFASLAVLTSCIVIWPFWEWGMRQTMQTPIDHPSRHNFFTDPLASAIFFWP
                     LYGPLVLIIPFIFQ"
BASE COUNT         1632 a         1596 c         1432 g         1574 t
ORIGIN      
        1 catattgcca atcaaaggat tcagcaacag ttcgaacgcc gtgatttggt ccatcctgct
       61 ccaaatcaat ggaaattacc aacggcactt tgaaatcaga aggaaatttt gccaacgcca
      121 gcgaggcgag cagccggctc aaaacatcag ggcggttata agcgggaata acgatcgcag
      181 gtatcatgct gggcatcaat tttgtttccg aatcacaacc caggggaaag gcgaatcgat
      241 ttgtccaatc cagcgcttca accgccgcgt caaactgccc ggatcgtaaa gcatctttgg
      301 atgcaaggaa tccagatcag agatgcgatg atggaagtaa tggtcaatca tttcggggca
      361 acccaggctg gcataatact cacggctcct taaaaccgca gtttctcttt ccctcttcga
      421 ataaagcgga gtatccagaa tgtgaatctc accattgggc tttagataat ccaataacat
      481 agttagtaaa ttcgtggcat tcggaaaata ttgtaaagca ctggcaacaa tgatcagatc
      541 gaaggatcca gccggaaatg gtgccgaaaa tatatccgct tcgagaaatg acaaatttgg
      601 atttgagcga aacacgcgcg cggcctgccg aagttcataa cgattttgat ccaatccaag
      661 cacgacctgc cctggctggg caagtaaatt agaaagccac ccatttccac agccgagatc
      721 gagaacgaaa aatggactct gccgagaaga gatataacga agcagacgtc gtgcggattc
      781 ggagcgagcg cgccattcag aaacaagcgg atgtccatct ggcacagatg gcaaaccagc
      841 tacaatttga tcagaatata accttccctc tttttcgcgg actctcaaat agagttcagg
      901 gtccatactg aaaggtatat gcgacgaaat ctccccaacg attgaaaggt gcacgacgcc
      961 cgaaatgttc atccatttgt aataggaatc gagtgaatga aggaaagcga tggggaaact
     1021 gatccatata ggaaggtgtg caaaacaatg aaatcgactg ttgtttcacg aggcgaaaat
     1081 tatgcccaaa tgctttgatc acttgttcag ggtggtaata ccacgtcatc aattgtgcac
     1141 cttcaacgtt agccaggact ccctcgcgat gcagtcgtcg cgtcgcaagg cgaaacttgc
     1201 ctcgcaaaac ctgagccagt tcccaggggc aaataggagg catgatcaca cacagcacat
     1261 gacctcctga tttaagcaac cgaggaatag tatttacaac gccatctaat gccggaatac
     1321 aattcagtcc accgaaattt gaaaatacca aatcatacgg tccgccctcg accaggtcta
     1381 actcggtgaa tgatagcttt tgaactgtaa actgatcagc gcgctggagt aatgctattt
     1441 ttttttggat ggcggcgacc ataccgctgg cgatgtcggt ggcgtgcact cgatatccct
     1501 gttgaacaaa atatgcggcg tcactgcccg tccctgcgtt aagctcaagg atggaagcat
     1561 tacctggcag gcgctgaact gcctcgtgcc gaatgacgtc ccgcgcccaa cgaatgactg
     1621 gatggttttc acagtactcg tcgtaaactt ccgctttgcg ggaaaagcca aggtcaacag
     1681 catttaagtc aagcattgac taccaaatta cgagacgtat ttgcgtactg tttcagtcgc
     1741 atttccgaga ataataatcc gatcaaatag taaggtatac gtatccagcg ttttggatcg
     1801 cgcttcaaaa tcgtctgtcg caggcgaaat tcatagtgaa cgcgtccatg cagaatacgg
     1861 taaaactccg ttgggaaagg cccgcgatag agcatcgcca gatcttcaga atcctgccag
     1921 tttcgttttt cgcccaattc caacttcact cgttcgaaaa actttgtgcc cggcaacgga
     1981 tacgatacag aaattccgat gtcatccggt gcgcactcgc gtaccatcct cagggtcttc
     2041 tgtacatcct cccacgtttc accgggataa ccaaattgca aaaagaatcc aacctcgatt
     2101 ccgttagagc gcaagagtcg ggctgcttca taaatatcat cgactgtgtc gcctttttcc
     2161 atggcgttta gaatcttttg tgagccagac tcggcaccaa cccacacggt cttgcatcca
     2221 gcttttgcca gagcggcagc cgttttctcg ttgaccaggt cggcgcgttg caaacacttg
     2281 aaagggataa ctgaatccat ctcttgtaat aacgccgcaa acttttcaag ccaatgcggc
     2341 ttcaaaccga gaatgtcatc ggcgaaccaa atgtggtcgg gcgcaaaatt ttccttgatc
     2401 catttcattt ccgcggcgac gttttcaggt gagcgcgagt tgtaacgctg gccccagatg
     2461 ggtttggcac accagttgca atgataggga caaccacgtg tggtcaccat attcatcgaa
     2521 aaaaaaccat gacgctccat ccagattgaa cgataggctt ggacgtccac cagatcccaa
     2581 gccggaaacg gcagtttatc gaggtttgtg atatccggac gacgcgggtt ctggacgatg
     2641 gacgatggac gatcaacgat ggtcaatggt ccgtcgtcca tggtttgtag tcccggccaa
     2701 gccaaacctt tgatcgactc tagagccacc tctcgcttgc ccgacatttg atccagcaat
     2761 tcgatcagcg tctcctcccc ttcgccgagc aggcaataat cagctccgcg cctgagatat
     2821 tcttcaggat ggtcggtcgc gtcggcaccg cataatatga cggtacatcc gattcgtttt
     2881 gccatctcga tcatcttgaa tgcagcctcg cgcattctca acaggcacat cttcgagaga
     2941 tagttgaaat tgtcttcgta gatgatcgca tattggggac gatgattttc caacgacgtc
     3001 ccccattcgt cttcggactc agccagcatc gcatcgaaca gagcgacctc gtatccgttt
     3061 tggcgcagat aactcgcagc atataatgtg ccaagtggcg ggtagggctg catcgcatcc
     3121 cacagtttgg gatcaaaccg aaggtagtac gattgaccaa ggaggatatg ggtcatcgta
     3181 aatttaggtg acagtcactt taaaggtgac tgccgactcc ttttcgagaa cagacaattt
     3241 attttcaaac atttcccgag tccacttgcg atgatgatga aaattaccct ggcaaacttc
     3301 ggcagtaaaa accgtttctt caccaaaccc ggcctgtttt gagaaacgcg caattttgcg
     3361 attcatttcc cactgttcga agcggctgcc aaaattgccc agcagaacaa attcaagcaa
     3421 agcttgaaaa gcaccagctg gcttacccac tgctggaagc gttttctcaa gctctgcata
     3481 agcattaggt agaaaccttt cgatccacct atttacctgc agtaacctgc gatatgtatc
     3541 aaatccagca atcggtatca tctggcataa ttcacgtgcc gagtacagat ctttttcggg
     3601 ccactcgagc gccagctcag agacaagtag gttcggacac aaggtatagc cctgcaaacg
     3661 cgccagccga tttaatgcta aggcaaaggc gcgagcgatc cataaccggc ccggggtagt
     3721 aacaagcata taatcgaaat ccgggtttcc tgagacattc atcacggcca gtgaaccagt
     3781 caacgctacc atgcgaatat aaggcaaatt acccagcatt cgtccatatg caatggcgct
     3841 cggcaacaac agagaggaat ggaatactcg ttgcatacga attcttacaa tttcttcgcg
     3901 accatgtaag gtaaaataac tatctgtttg cccaacgacc ccatcttctc tcaaagcgcg
     3961 aaccacttct tccagcgagg cttttatacc cgtaagatat cgatggattt cacgcgcgct
     4021 caacggataa tcaaatagat ccgagtaggc aactgtctgc aaaatcgcgc gcgataaatc
     4081 tcgtgaagaa tggtccagca taaaaatcca attacgcaga attaacgtaa atagtacata
     4141 gaactataca acgctcaaga ggttctgtca aaccacatca tgcacctgac gatcgtcagc
     4201 ccgttccccc ctacaattac cggcattggg cagtatggat atcatgtcac tcggtccctg
     4261 gcaaagtcag gctcgttttc gcgcgtcact gtactggcag gttcaaacgg taacggagaa
     4321 caaccgaatc atctcggcac aaccgaattg gactactgct gggagccggg tcaattaaat
     4381 gcgcggcgcg ccatcgtatc ccgcataaag cgactgcgtc cggaactcgt ctggttcaat
     4441 cttggagcaa gtgtctttgg aaaatctccc tggaccaact tatctggctt gtttacaccc
     4501 atgttgatcc agcgtctggg gatacccacc gtgatcactt tgcatgaatt agcagagcta
     4561 tctgaccttc gggcgttgaa cgcgcccgga ggtccttttg cgcatttagg ggcgcgcgtg
     4621 attactggta ttgcaacgcg ggcggatgtg acctgcctga cgatgcgcaa ttatgcagac
     4681 tggctgagtg taaaacagat cacctgcaca cacattccaa ttggtgcata tcacgagcca
     4741 aatctgttgg aggaatctga ttcacaggaa ctgttgtttt ttacaacatt ggctccgttc
     4801 aaagggctgg agttgttatt ggaagccttc cgtgttttga gaagggaatt tcctgcattg
     4861 cgattgacca ttgcaggcac ggaacatgcc cgctttccga attatgccca tgaattgaga
     4921 acacatttca acggaaccga aggaattcat tggcgaggtc agattgcaga agaccatgta
     4981 atggagctat ttcgcaaagc ccagatcgta gtcctgccat acacagcctc gactggctca
     5041 tccagcgttc tgtatcaagc cgcgacctgg ggacgccccg tcgtagtatc cgatttgaaa
     5101 gagatccgtg cattagctaa ggaaagcaat ttgctgctgg aatttttcaa gaacggtgat
     5161 ctccagagct tatgtcagtc tttacgtgcc ttactcaatt cccccgagaa gcgccgcagg
     5221 caggcctggg ctaatttcac atccattcaa catgcacgcc ctgaggtcac ttgcagaaaa
     5281 tatatcgagg ctttcaatta cgccctgaga aagcgcggaa atgaaaaccg cattcgtatc
     5341 ccatctacta cattggaaac aggatgattc cccggcgcgt taagattgca atcctcatcg
     5401 cttttctatt tcatgggctt ttcatcctta ctggtcgtta tcgattgagt tatgacgcat
     5461 atactcacat tctttttgca aatcattacg ctgagaactg gttctcgcta tgggagccgc
     5521 gctggtatac gggctttacg gtcgtctcct atccccctct gattcatcaa ctgatcgcgc
     5581 tgtttattcc tttgctcggc ttcgagggag cctttgcgct catgctttgg gtagtcacaa
     5641 cgttataccc cattggtctt tacggattaa gccgagtttt cgtaggaaaa tccgcatcgt
     5701 cgtacgccgc gcttttttcg gcagcgctct taccgcttta cgtcgttgcc catatttttg
     5761 gtcaattgcc ctttctggcg agtacgctga tcgctttatt tggcgcggta agccttaacc
     5821 gatatcttcg tgaaggcgga attcttaatt tactgttgac cgtttctctc ataacaacct
     5881 cgattgcggc tcatcatgca acgctcatga ttcatccgtt tctggttttc gctgtactga
     5941 taaaaaatat agcgaaccac agatcaatta accgtcttca agaaggccgc tcccagatct
     6001 ttgtgacctg gtggtttgcg ttgttcagtc gactgatgct gtttgcttca ctggcagttc
     6061 tcactagttg cattgtcatt tggcccttct gggagtgggg catgcgccaa accatgcaaa
     6121 cgcctattga ccatccgtcg cgccataact tttttactga tccgttggcg tccgcgatat
     6181 tcttttggcc tctatatggt ccacttgtac tcatcatccc atttattttt caac
//
LOCUS       DASTJP010000500         7419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_926766, whole genome shotgun sequence.
ACCESSION   DASTJP010000500 DASTJP010000000
VERSION     DASTJP010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926766"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..561)
                     /locus_tag="VFQ23_14525"
     CDS             complement(133..561)
                     /locus_tag="VFQ23_14525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET9907863.1"
                     /translation="MTVCIFCQIVEGILPASKVYEDEICLAFMDIQPVNPGHILVIPK
                     VHFEDLADLPGDTGAHLFQVAQRIVLSLPKTDIKMEGANLFLAHGEAAGQEVFHVHLH
                     VIPRFASDGFGFRFGPNYSNLPERHQLDAIATQIKQQLEN"
     gene            complement(558..1592)
                     /locus_tag="VFQ23_14530"
     CDS             complement(558..1592)
                     /locus_tag="VFQ23_14530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9907864.1"
                     /translation="MKLDAALPPVGLRDVPAIAEAAEEIGFDALWTQETQHDAFLPCT
                     LIAEHSTRLNFGTAIAVSFARSPANLAYTAWDLAAQSNGRFILGLGTQVKAHIERRFG
                     QPWPESPVKKLREQIQVIRAFWDCWQNGTKLNFRGEYYKITLMSPFFRPPPLPSPIFL
                     ENGGGWGGGIPIYIAGVNTGLARLAGELCEGFHVHPFHSTRYLKEVILPGIQEGAQKA
                     GRDRSDVSISVTAFAASSSEEINFARAQISFYASTPSYRPVMDLHGWGSVAEKLSTHA
                     SRGEWAEMPMLIADEILSEFCLMTSEDKLADELKKRYIGLADRLALYTPFVPGEKDKW
                     WKGLSGEFNL"
     gene            complement(1703..2749)
                     /locus_tag="VFQ23_14535"
     CDS             complement(1703..2749)
                     /locus_tag="VFQ23_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET9907865.1"
                     /translation="MSLPPINQEYFTDFLVKLLNIASPTGFAEPAIAFVENELSQFSQ
                     LTLSRTRKGALVAKWEVESDLPPVALTAHVDTLGAVVKEIKGNGRLKLSRIGGIQWNT
                     VETEGVWVFASKGEKVRGSVLIDTASGHVHGAAGSETKRNDEHMEVRLDVKTISEKET
                     RALGINVGDCVAFDPRVEVTNGFIRSRFLDDKACVANLISAIKSLVDAGQSPTRTVYF
                     HISNYEEVGHGAAAGIPPEVAELVTVDMAVVGAGQESDEFHATLCTKDGGGPYHFGLN
                     NKMRALAEKHNIPYKTDVYVYYGSDGEAFWSAGGDVALALIGPGIDASHNYERAHMDG
                     LNATTNWIMAYLLD"
     gene            complement(2890..3327)
                     /locus_tag="VFQ23_14540"
     CDS             complement(2890..3327)
                     /locus_tag="VFQ23_14540"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9907866.1"
                     /translation="MSQFRFYHPVEVRYGDLDPQGHVNNAKHLTYFEQARIAYLLELG
                     LFTKDQSFMEIGVILADLHITYLEPVYFGQNIKVGVKVAKLGYKSMNWKQNIIDADAD
                     KEIAKGEIVMVTYDYREKKTIPIPQAWRETISRFENLSVVKKE"
     gene            complement(3361..4125)
                     /locus_tag="VFQ23_14545"
     CDS             complement(3361..4125)
                     /locus_tag="VFQ23_14545"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET9907867.1"
                     /translation="MFIQPNKTKAKLRAGQPAFGVLSTTDDPQLAELFGIAGFDYYML
                     DAEHGLMDPAQVMNVIRTCELTDMTPMIRIGSKDPKIVLQYLDAGMMGIMMPGLETPE
                     EVRMLVDAMKYAPIGKRGLGFSRASGHMAYTSTATEYINFSNEQIMVIIQFEDEKLLK
                     IFPELLSVEGLDACVVGPRDLSNNMGFADGPNHPEVQAVIDDAIGIINKSGVIAGITA
                     GTRADAENQVARGATMILAAAQFLVLSGASAFLPEK"
     gene            complement(4157..4855)
                     /locus_tag="VFQ23_14550"
     CDS             complement(4157..4855)
                     /locus_tag="VFQ23_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9907868.1"
                     /translation="MVDFKKDDTRNWLSYIKLFEGLTPTQLDWVAQRAHRRVFEAGRN
                     VLTIEQPGEAVFIILHGTVKIHIEQSERDVILAILGSGDMLGEMSLIDSVGRSASAVT
                     LESSLLLWMDKTTFNYILDNFPPVARNLVKIMSARVRLSDQLIQALATLDVNGRVARQ
                     LLAFAEKYGEEKNGGTKISIVLTQSDIADLVGASRKRVNQAMVSFKELGLIDTDANGR
                     IEIKDSEQLAKFCK"
     gene            complement(4868..5842)
                     /locus_tag="VFQ23_14555"
     CDS             complement(4868..5842)
                     /locus_tag="VFQ23_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9907869.1"
                     /translation="MSNELKVKFWGVRGSYPTPGAGTIKYGGNTASVEVRAGERTIIL
                     DAGTGIIPLGRELARTKRAGQIMLLLSHLHHDHTQGFPFFVPAYLPNTKIHIFGPDGT
                     HESMKNVLERNQSSETFPLGLRDMASFKDIQAVRESQVIVCEENGIRFAESGAGVSDE
                     AVVVRIHKSYAHPGSVYVYRISWRGKSIVYATDTEGYVGTDRKLVQFARDADVLIHDA
                     QYLEEHYRGQLVGFPSTQGFGHSTVTMASEVALASDAGRLVLFHHDPSYTDEIIAGME
                     TTARSLFADSVAAFEGLEIVLRDEKKMAMQLQTSSPLPLSHRERRQWK"
     gene            6036..6272
                     /locus_tag="VFQ23_14560"
     CDS             6036..6272
                     /locus_tag="VFQ23_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907870.1"
                     /translation="MAVIRSSDIGNYLYCRRAWWYKKQGLVSENQSELSAGTELHRKH
                     GRQVVASGISRSIGLILLMVALVMLVAYCTSQLL"
     gene            6329..6958
                     /locus_tag="VFQ23_14565"
     CDS             6329..6958
                     /locus_tag="VFQ23_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907871.1"
                     /translation="MSVLNRLAHSLGRRDEVPNQELARDLVAKKDKQGIQEIAENLWN
                     KDKNIQADCIKVLYEVGAIEPSLITNYTEDFVKCLRNKNNRLAWGGMTALAEVAKTDP
                     DSVFKHLDVIKKAKETGSVITVDNAISTLAYTAAANKKYNDAIFPYLLQHLAGCRPKE
                     VPQHSEKTMPAVNVANKTEFVRVLEKRMEDVSGSGLSRVKKVIKQANQI"
     gene            6967..7419
                     /locus_tag="VFQ23_14570"
     CDS             6967..7419
                     /locus_tag="VFQ23_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907872.1"
                     /translation="MSKIIEFFLGSILIAVFTIALIVFSALHFVFKAGGIIDCEWHGS
                     ARTWIDLNGDGFVNNDEPPLGNVNIHVDEIHNQRAIVGWPAVTNKDGIVQLQVPMPGC
                     PNMDFEVYVADTPEGYRLTTAPRFDVNRDSWESLDSNRVYYFGFIPEQ"
BASE COUNT         1834 a         1874 c         1755 g         1956 t
ORIGIN      
        1 tcttgatctt tcctgcaact tctcccgcct cattgaccaa tccaagtgtg ggatagataa
       61 caccatgccc gatgactggg tatttggctg tagcgcgtga tttagtttga taatcgttga
      121 agttcatgtt agttaatttt ccaattgttg tttaatttgc gtagcaatcg catctaattg
      181 atgtctttcc ggtaagttcg aataattcgg accaaatctg aagccaaatc catcgcttgc
      241 gaatctggga atcacatgca ggtgcacatg aaaaacttct tgcccggccg cttctccgtg
      301 cgccaggaaa agatttgcac cctccatctt tatatcagtc tttggcaggc tcaaaacaat
      361 gcgttgtgcc acctgaaata gatgtgctcc agtatctcct ggaaggtcag caagatcctc
      421 aaagtgtact ttcggtatga caaggatatg ccccggattg acaggttgga tatccatgaa
      481 cgccaggcag atttcatctt cgtaaacttt actggcaggt aagatacctt ccacaatctg
      541 acagaaaata caaactgtca taagttgaat tctccagaca gacctttcca ccacttatct
      601 ttctcaccag gaacgaaagg tgtatataag gctaatcgat cggcgagtcc aatgtagcgt
      661 ttcttgagtt catcagctag tttatcttca gatgtcatca gacaaaactc actcaagatc
      721 tcatctgcaa ttagcattgg catctcagcc cactcgccgc gcgacgcatg agtagagagc
      781 ttctctgcca cactgcccca accgtgcaga tccatcaccg ggcgatagga gggcgtagaa
      841 gcgtagaagg agatttgggc gcgagcgaaa ttaatctctt cggaagatga agccgcaaaa
      901 gccgtaacgg atatagagac atcacttcga tctctgcctg ctttttgtgc accttcttga
      961 attccaggca ggatcacttc tttcaaatat cgcgtgctgt gaaacggatg cacatggaag
     1021 ccctcacaaa gttcccccgc gagcctggca agccctgtat tgacacccgc gatgtaaatt
     1081 ggaatgcccc caccccaccc tcccccattt tcaagaaaaa tgggggaggg caagggaggg
     1141 ggccgaaaaa acggtgacat caaggtgatc ttgtaatatt cccctcgaaa gttcaatttc
     1201 gttccgttct gccagcaatc ccaaaaggcg cggatgactt gaatctgctc acggagtttt
     1261 ttcaccggcg attcgggcca cggctgaccg aaccgccgct ctatatgtgc tttgacctgg
     1321 gtgccgagtc ctagaatgaa tcgtccgttg gattgggcgg ccaaatccca ggcggtgtag
     1381 gcgaggttcg ctggggagcg cgcaaaggaa accgcgatcg ccgtcccgaa gttgagacgt
     1441 gtgctatgtt ccgcgattaa tgtgcagggc aggaaggcat cgtgttgagt ctcctgagtc
     1501 cacagcgcgt cgaatccgat ctcctccgca gcttcggcaa tcgcggggac gtctcttaat
     1561 ccaacgggtg gaagggcggc atcgagtttc atactattgt tctgtcattg cgaggagcga
     1621 accgctttac ggtgagcgac gaagcaatct cgtggttata ggttagttga gattgctacg
     1681 ggctatcgcc ctcgcaatga cattagtcaa gcagatacgc cataatccaa tttgttgtcg
     1741 cgttcaatcc atccatatgt gcgcgttcgt aattatgtga cgcgtcaatg ccgggaccga
     1801 tcaacgccag cgccacatcg ccacctgcgc tccagaaggc ttcaccgtct gaaccgtagt
     1861 acacatacac gtcagtctta tacggaatgt tgtgtttttc agcgagcgca cgcattttat
     1921 tattcaatcc aaagtgatag ggtccgccgc cgtccttggt gcacaacgtg gcgtgaaact
     1981 catctgactc ctgtccagcg cccaccactg ccatatccac cgtgaccagt tccgccacct
     2041 cgggtgggat gcctgccgca gcaccatgcc ccacttcttc ataattggat atatgaaaat
     2101 aaacggttcg tgtgggactt tgacctgcat ctaccaatga tttgatcgcg ctgatgagat
     2161 ttgccacgca tgctttgtcg tccaggaagc gggagcggat aaagccgttc gtgacttcca
     2221 cgcgcggatc aaaggccaca caatcgccga cgttgattcc cagtgcgcgc gtctctttct
     2281 ctgatatggt ttttacatcc aaacggactt ccatatgttc atcgttgcgt ttggtttcgc
     2341 tccctgccgc tccatggaca tgaccggagg ctgtgtcgat caacaccgag ccgcgaacct
     2401 tctcaccttt ggaagcgaac acccagacgc cttccgtttc tactgtattc cattgaattc
     2461 ccccgatccg acttaactta agccgcccgt ttcccttgat ctctttcacc actgcgccaa
     2521 gagtatccac atgtgcagta agggcaacgg gaggcagatc ggactctact tcccatttcg
     2581 cgacaagagc ccctttgcgt gtgcgcgaca aggtcaattg gctgaactgt gatagttcat
     2641 tctctacaaa tgcaatcgca ggttcggcaa agcctgtcgg cgaggcgatg ttcagtaact
     2701 tcacgagaaa atcagtaaaa tattcctgat taataggtgg caacgacata gttttcctct
     2761 tttccaaaat ggaccgttgg tcgagtaggg cggagcccat atcgagacca acagatttca
     2821 aaagtaatct tataacaaga attgttttgt ttggtggtgg tttcgatacg cccttgacta
     2881 gcactcaggc tactcttttt tgaccaccga tagattttca aacctggata tcgtttccct
     2941 ccatgcctgt ggaataggaa tggtcttttt ttctcggtaa tcataggtca ccataacgat
     3001 ctcccctttg gcgatttcct tgtctgcgtc tgcatcaata atattttgct tccagttcat
     3061 cgacttatac cccaactttg caaccttgac tccgactttt atgttctgcc caaaatatac
     3121 aggctccaga taggtgatat gcaggtcggc caggatcacg ccgatttcca tgaaggattg
     3181 gtcttttgtg aacaatccta attccagcaa ataggcgatg cgggcctgtt caaaatacgt
     3241 aagatgtttt gcgttattga catgcccctg tggatccaaa tccccatacc ggacttcgac
     3301 aggatgatag aagcggaatt gtgacattcc gcgattatac ctatcttcga ctgtagattg
     3361 ctatttctct ggcaaaaaag ctgaggcgcc tgacagcaca aggaattgtg ccgcagcaag
     3421 gatcatcgtg gcaccgcggg caacctgatt ctctgcatcg gcgcgagtgc ctgcggtgat
     3481 cccggcaatg accccactct tattgatgat gccaatcgca tcatcaataa ctgcctgcac
     3541 ttcaggatga ttgggtccat cggcgaagcc catattgttg gaaaggtcgc gtggtccaac
     3601 gacacatgca tccaaacctt caacagacaa taattcagga aatatcttga ggagtttttc
     3661 atcttcaaac tggataatga ccatgatttg ttcattcgaa aagttgatat attcagttgc
     3721 cgtgcttgta tacgccatat gaccggaggc gcgagagaaa cccaggccgc gcttgccaat
     3781 cggagcgtat ttcatagcat ccacaagcat cctgacttcc tccggcgtct ccaaccctgg
     3841 catcatgatg cccatcatgc ctgcatcgag atattgcaaa acaatttttg gatcttttga
     3901 gccaatgcgg atcatgggag tcatgtctgt caattcacat gtgcgaatta cattcataac
     3961 ctgcgcagga tccatcaatc cgtgttcggc atccagcatg taataatcga agccagcgat
     4021 gccgaagagt tcagcgagct gtggatcatc ggttgtagag agtacgccga aggcgggctg
     4081 cccggctctc agttttgctt tggttttatt tggttgaatg aacatgagat tccagccgga
     4141 ccctttctct tttgttttac ttacaaaatt tcgctaactg ttcgctatcc tttatttcaa
     4201 ttctgccatt tgcatcggta tcaatcaagc ccagctcttt gaacgacacc atcgcctgat
     4261 ttactctctt ccgcgatgcg ccaacgagat ctgcgatatc gctctgggtc agtacaatgc
     4321 tgatctttgt gccgccattt ttctcctcac catacttttc ggcaaatgcc agcaactgac
     4381 gcgccacgcg tccgtttacg tccaaagttg ccaacgcctg gatcaactgg tcagaaaggc
     4441 gcacccgggc cgacatgatt ttcaccaaat tacgcgccac aggcgggaag ttatcgagaa
     4501 tgtaattgaa ggttgtcttg tccatccata agagcaaact gctttccagc gtcacagcac
     4561 tggctgaacg gccgacgctg tcaatcagac tcatctcacc gagcatatca ccgctcccca
     4621 gaatcgcgag aatgacatcg cgttccgact gctcgatgtg aatctttacg gttccatgca
     4681 aaatgataaa cacggcttcg ccaggctgtt cgatggtcaa tacgtttcgt ccggcttcga
     4741 atacgcggcg atgcgcgcgc tgtgcaaccc aatccaattg agttggcgtg agtccttcga
     4801 acaacttaat atacgaaagc cagttgcgag tatcgtcttt tttgaagtca accattctca
     4861 gcatatttta cttccattgt ctgcgttccc tgtgggagag gggaaggggt gaagacgttt
     4921 gtaattgcat tgccattttc ttttcatcac gcaacacaat ctccaatcct tcaaaggctg
     4981 caaccgaatc agcgaacagg ctcctggcag tagtctccat gccagcgata atttcgtctg
     5041 tgtaggatgg gtcgtggtgg aacaggacca atcgtccagc atccgaggcg agtgctactt
     5101 cagaggccat agtcacggtg gagtgtccga atccctgcgt ggatggaaac ccgaccaatt
     5161 gaccgcggta atgctcttcc aaatattgtg catcatgaat caacacatcc gcgtccctgg
     5221 cgaattgcac cagcttccgg tccgtgccca catacccttc cgtgtctgtg gcgtacacaa
     5281 ttgatttgcc gcgccaggag atgcgataaa cataaacact gccggggtgc gcataggatt
     5341 tatgaatacg aacgacgacc gcctcatcac ttacgcccgc acctgattcc gcgaaacgga
     5401 ttccattctc ctcacagacg atgacctgcg actcgcgcac ggcttgaata tccttgaagg
     5461 atgccatatc gcgcaaccca agagggaacg tctctgatga ttggttgcgt tccaacacat
     5521 tcttcatgga ctcgtgcgtg ccatccgggc caaagatatg tattttggta tttggcagat
     5581 aagctgggac aaagaagggg aaaccctggg tatgatcatg atgcaggtgg ctcaaaagca
     5641 acatgatttg acctgcgcgc ttggtccgtg ccagttcgcg tcccaatgga ataatccctg
     5701 tgccggcatc gagaatgatc gtgcgttcgc cggcgcgtac ttcgacgctg gctgtattgc
     5761 ctccatattt gatcgtcccc gcgcccggtg tgggataact cccgcgtaca ccccagaatt
     5821 tgacttttag ttcgttagac atcacagcac ctccgtgtcg ctttctgttt ttattatcct
     5881 cactaaaaga ttttcctcaa ggaaagtttt cgcaacggaa gtgggaagtt tttcacaaga
     5941 agagttacag tttttgcgtt taatatatat gcctacagtg caggggcttt agatcccaaa
     6001 ggaaaaaatt agaaaatttg tgctatactt taccgatggc tgtgattcgc tcgtctgata
     6061 tcgggaatta tctctattgt cgccgcgcct ggtggtataa aaaacaggga ttggtttcag
     6121 agaaccagtc ggaactctct gccggaacgg aattacatcg caagcacggg cgtcaggttg
     6181 ttgcctccgg aatttctcgc tccattggct tgatcttatt gatggtggca ctggtcatgc
     6241 tggtcgccta ttgcacgtca caactacttt aagggtgggg ttttcccatc ctagggcata
     6301 cgttgggtgt tgcccctacg gagaaaatat gtctgtcctt aaccgtctcg ctcattcgtt
     6361 aggtcgacgt gacgaagttc ccaatcagga actggcacgg gaccttgtcg cgaaaaaaga
     6421 caaacagggg attcaagaaa tcgcggaaaa tctatggaac aaagacaaaa atattcaagc
     6481 tgattgcatc aaggtcttgt atgaggtggg tgccattgag cccagcctga tcacaaacta
     6541 caccgaagat ttcgtgaaat gcctcaggaa taaaaataat cgtcttgcct ggggaggaat
     6601 gactgccctg gcagaggtcg caaaaactga tccggattca gtcttcaaac acttggatgt
     6661 gatcaaaaaa gcaaaagaga cgggctcggt cattacggtg gataacgcaa tttccactct
     6721 ggcttataca gcggctgcaa ataagaaata taacgatgct atttttccgt acttgcttca
     6781 gcatttggcg ggctgccgtc caaaggaagt gccgcagcat tcagagaaga cgatgcctgc
     6841 tgtgaatgtt gccaataaaa ctgagttcgt ccgagtcctt gaaaaacgga tggaagatgt
     6901 gtctggttct ggtttatcgc gcgtaaagaa agtcattaaa caagccaatc aaatttaagg
     6961 ttcgatatga gcaaaataat tgaattcttt ttgggttcaa tattgattgc ggttttcacc
     7021 atcgccctga tcgtcttctc ggcccttcac tttgtattca aggctggtgg catcatcgat
     7081 tgtgaatggc atggttccgc caggacgtgg atagacctca acggggatgg atttgtcaat
     7141 aatgacgaac caccgcttgg aaatgtaaat attcacgttg atgaaataca caatcaacga
     7201 gcaattgtag gctggccggc tgttacaaat aaagatggaa ttgttcaact ccaggtgccg
     7261 atgcccggat gtcccaatat ggactttgag gtgtatgtgg cagacacacc cgagggctat
     7321 cgtctcacca ccgcacctcg ctttgacgta aatcgggatt cctgggagag tttggattca
     7381 aatcgtgttt attattttgg ttttatccct gaacaatag
//
LOCUS       DASTJP010000501        13298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_121380, whole genome shotgun sequence.
ACCESSION   DASTJP010000501 DASTJP010000000
VERSION     DASTJP010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121380"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFQ23_14575"
     CDS             <1..580
                     /locus_tag="VFQ23_14575"
                     /inference="COORDINATES: protein motif:HMM:NF014787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907873.1"
                     /translation="TPSAAPLSSIVPAQALKELARVANDAEEPIYMVLPKGRGQVVFR
                     VKDVEIVSQLIDGTFPDYQQIIPRSYKSRTLVSTSSLLKACKQAEIFAREGSNVARFN
                     IKSAQGEMQPSEVEISATSEETGKNETIVEATVDGGGLLIAFNVKFLREALEVIRTPN
                     VALETSAPNAPGVVKPVGDDQFLHVIMPMHLG"
     gene            complement(629..1873)
                     /locus_tag="VFQ23_14580"
     CDS             complement(629..1873)
                     /locus_tag="VFQ23_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9907874.1"
                     /translation="MTSTGRLRDLEGQQQQLLRLVELSVTLNSTLNLETLLQVITGTA
                     TELLDCEGASILLYDEKNPRLYFAAATGSDPAKLAEIPVPMEGSLAGTIFRTNRPIII
                     NNAEQDPRHFALVSEHIKFKVKTLLGVPMPIKDRTVGVLEAINKRNGVFDERDATILS
                     VTAAHAAIAINNAQLLRTTQMALEKVKDANELKSNFLALASHELRTPLGIIIGYSTFL
                     KEDAKGELSEHATQVLNAAGQMRSILDEMNNLAMLKSDQMNLKPQRIVMQDVLTYACE
                     GIKELAAARDQKLTYVLPEEPFFVYVDIDRTALALGNVLNNAIRFSPPGSKITVGTIK
                     QGNQILAWVQDQGIGIPTDKLQKIFEEFYQIEPPNTRNYGGLGIGLTIAKGLIEAQGG
                     KIWSESEGLGTGATFKVMLPVV"
     gene            complement(1918..3372)
                     /locus_tag="VFQ23_14585"
     CDS             complement(1918..3372)
                     /locus_tag="VFQ23_14585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9907875.1"
                     /translation="MGELRNPFLIIILLLSVVIGSSHLTRGHEWGDDFASYIMQSESI
                     VNGEMQEFVEHNNITIHQSSSQTGPVAYPWGYPLILTPAYMIKGISPLTLKIPALIFF
                     AGFSICLFLLVRNHFTRIESLLFVSLFAFNPLLINFLDQILSDIPFLFFSTLALLLMT
                     KENKRGIPHHVLIGVLIAFAFFIRTVGILLLASFLIVETYRAWSDRKNRQLFIKILQN
                     IFMVCMSFGVLWLMYSLIFPGGSESYFDQLRGFKLEIALQFAIDYFQLFSAFFGESRV
                     WQYLYYLLFIFFLIGLWSRRRDDFALIVFFALWMLMLITWPIWQGPRFIFPLIPIFIY
                     FTFQGMKLVIKSLPEASHQTAQKAFLGFWILLAGIFLVNSSLGAAVNLRNSRSINGPF
                     DPFSLQMYDYIREKTSSDSVIVFFKPRAMRLMTDRDAIMSTECSRMSLGDHIVLSRKV
                     EENQQISPEEIASCNLPLEEVFRNRRFIIYDILK"
     gene            complement(3502..3723)
                     /locus_tag="VFQ23_14590"
     CDS             complement(3502..3723)
                     /locus_tag="VFQ23_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907876.1"
                     /translation="MSEEINQWEYRVQTIGSTFGTKDEDIEATLDAWGEEGWEAINVY
                     TPYGSGKVTIIAKRPLTGRARRMRTMPQS"
     gene            complement(3736..4527)
                     /locus_tag="VFQ23_14595"
     CDS             complement(3736..4527)
                     /locus_tag="VFQ23_14595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9907877.1"
                     /translation="MKTILNWRVFFILWIASIVSSIAVLPYVLELQGAALEAMDLPLS
                     LPALLALQIVQAAILFAIMIVAGMFFASRVGLGTPILDSLTRRESVSDKIRALLPISI
                     ALGVLAGMLIIGIDYFLQPALLKELGDSASALDSQNIHPAAWKGFLASFYGGIAEEIL
                     LRLLVMSFFAWLGRFISKTSEGKPSATVFWVANIFAAVLFGLGHLPATAMLLPLTPLV
                     VTRAILLNGIGGVIFGWLYWKRGLEAAMIAHFSADIVLHVLLASL"
     gene            complement(4571..5221)
                     /locus_tag="VFQ23_14600"
     CDS             complement(4571..5221)
                     /locus_tag="VFQ23_14600"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HET9907878.1"
                     /translation="MSTRTTTILVLVMLIGATIAGLLLWNRLPEQVASHWNFNDEVDG
                     YMPRFWGVFLMPLITLGMLVLFLVIPAIDPLKANIAEFREAFNLFIVLIIAFLLYVYG
                     LTLAWNVGYTGFRISTSLLPAIGLLFIFIGFMLRQARRNFFIGIRTPWTLSSDRVWEK
                     THRIGSTLFMLAGAFAIIGSFFGGATAFWMLMIPIFGSTIFLVIYSYILYKQETNV"
     gene            complement(5246..6190)
                     /locus_tag="VFQ23_14605"
     CDS             complement(5246..6190)
                     /locus_tag="VFQ23_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET9907879.1"
                     /translation="MDSKFIKSVNQKKTSKDKDLHVSGVAGTIFGIFLTIAVLGAILM
                     DGRVNGAWIGLHVLGMSLIGTYILFALKVASQWEKAIVLRLGKFLRLRGPGLFWIIPI
                     IDAIPAWIDHRVMVTPFAAQRTLTKDTVPVDVDAVLFWVVWDAEKAALEVENYRAAVD
                     WAAQTALRDIIGRTMLAEILVGRSVIDKELQQIIDERTTPWGVTVQSVEIRDIVIPQD
                     LEDAMSRQAQAERERQARVILGESEKQIADSFSQASQAYIDNPTALHLRAMNMLFEGL
                     KEKGALVIVPSSAVDTMNLGAMSGLVSIGQNNLPKDNK"
     gene            complement(6205..6627)
                     /locus_tag="VFQ23_14610"
     CDS             complement(6205..6627)
                     /locus_tag="VFQ23_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9907880.1"
                     /translation="MPSQEPLTLQLDFRSGLPIYIQIMNQIEGQVLGGTLKPGDQLPT
                     VRALASELRVNFNTVARAYRMLDEARIISTQQGRGTYITEVPAPEVTERLRKEMLEAL
                     ARKYIGEALRLEFSKKEIGQMVRGQLKDREESQMSDSE"
     gene            complement(6753..8225)
                     /locus_tag="VFQ23_14615"
     CDS             complement(6753..8225)
                     /locus_tag="VFQ23_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9907881.1"
                     /translation="MRIPLDRQSSIPLYQQIETYLRQGIISGSLPADTRLPASRQLAR
                     DLGVNRITVENAYAELETEGLIYSRMGSGTYVLPTNSLLTLSKDGPNATWPLWQQSVE
                     LQDKSSKNKPQNKASRHKTSHRDPIHFDSGIGDAHLFPAEEFRKVLQTVMRRDGIAAL
                     DYGEPNGHAPLRGTIAHILTSQGVQTRAENVLITAGSQQALSLVSQLLLKPGDVILVE
                     SPTYSGALDLFRSLGFKVVGIPVDAQGMQVEMLEKLLQQHHPKLIYTIPNFHNPTGTC
                     LSSPRRRDLIVLADRYNVPILEDDFVGDLRYEGRTQPALKALDPGGRVIYINSFSKIL
                     MPGLRVGFLVAEGPIYDALVNFKRVNDLATSTLVQRALEAYVTVGRYQAHLRRSCQIF
                     RKRRDAMLAAVKQYLPAEIHLDIPQGGLFLWLQLPDDLASEKLLPVALEEGVVFAPGN
                     EFFPDRVGGINWVRLNFVAQAPDQIDEGIKRLGIAIRSFV"
     gene            8464..9351
                     /gene="pdxS"
                     /locus_tag="VFQ23_14620"
     CDS             8464..9351
                     /gene="pdxS"
                     /locus_tag="VFQ23_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET9907882.1"
                     /translation="MEQQTGTFAVKKGLAQMLKGGVIMDVVTAEHAKIAEDAGACAVM
                     ALERVPADIRANGGVARMSDPDLILKIMDSVTIPVMAKCRIGHFVEAQILEAIGVDYI
                     DESEVLTPADEANHILKHNFTVPFVCGCRNLGEALRRVGEGAAMIRTKGEAGTGDVVE
                     AVRHARTVLGDIRRLTTMADEELMSYAKEIGAPYELIKETKELGRMPVVNFAAGGVAT
                     PADAALMMQLGVDGVFVGSGIFKSGDPSRRAAAIVKAVTHYQDANILADVSKDLGEPM
                     VGRNVSQMPESEKIAQRGW"
     gene            9429..10007
                     /gene="pdxT"
                     /locus_tag="VFQ23_14625"
     CDS             9429..10007
                     /gene="pdxT"
                     /locus_tag="VFQ23_14625"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET9907883.1"
                     /translation="MKIGVLALQGDFAEHIAMLKNINADAVEVRLPDQLKGLDGLIIP
                     GGESTTIGKLAVAYHLMEPLKEFGQRHAIWGTCAGAIFLSKDVSRDQPLLGLMDIKVE
                     RNAFGRQVDSFEADLDVPELKQATGTDEDYHAVFIRAPIIESVSGDAKILASAPDGRI
                     VAAQQGHLLATSFHPELTNDTRFHQYFLSLVD"
     gene            10166..11203
                     /locus_tag="VFQ23_14630"
     CDS             10166..11203
                     /locus_tag="VFQ23_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET9907884.1"
                     /translation="MHYKLAFIGFGNVARSLVRLLERKRDLLKREYDITYSITGIATG
                     RHGFAVNPDGIDEQQALALVESGKSINPLSTFQVDDSLAVIQHAQANVMFENSPVNYE
                     TGQPAIDHTKAALNAGMHVSTANKGTVVHAYQELTSLAKSKGKKFMFESTVLGGTPVF
                     STFREAMPLAELMSFKGIINATTNVILSRMEDGESFEDAVKYCQSIGIAETDPSGDVD
                     GWDASIKVAALATVLMNTPLKPQDVERKGIREITPEMVNEAKANGRRWKLVASAERVG
                     SQIRARVAPELVDASSPLYGMMGSSTGLTFRTDVLPDYSITVSEREGMKGGPEETAYG
                     LFADFVNAVKS"
     gene            complement(11198..12025)
                     /locus_tag="VFQ23_14635"
     CDS             complement(11198..12025)
                     /locus_tag="VFQ23_14635"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907885.1"
                     /translation="MREVEGIPGYSKEEVIHQVELNLWETWANFGRGPGCTLHDEDDA
                     LWFETPIPTMPYNTVLRFQVEKDIDQRIGSLVKGYADRNVTQLWIVHPTALPTDLPQR
                     LVEHGLQEIEIAPCMARGLDDLPEAPPVPPGVEIREAIEESDLIELYGLAAWRWGVPE
                     EYRLQLRKMIERFEIGERNSNTRMWLALKDGVPISKIGLYNGSGSAGIYGVATKPEAR
                     GLGIASILMNVAMKAAKEMGHKLAVLDSSPLAEKLYLRLGFITVAPLRLYSPIAAYL"
     gene            complement(12102..12878)
                     /locus_tag="VFQ23_14640"
     CDS             complement(12102..12878)
                     /locus_tag="VFQ23_14640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9907886.1"
                     /translation="MSDRNNAFNFEDHFSKQAETYAQYRPQYPDEIYAYLASVSPGLS
                     LAWDCGTGNGQAAIGLAKHFDKVHATDASAEQISRAYQKEKVEYRVEPAEHVSLNDSS
                     TDLVTVAVAIHWFNFDEFYREVKRVLKPNGVIAAWTYNVTEITPEIDQLTEEYYKGIL
                     DGYWPERIRYLEEKYETLPFPFEEIVRPAFVMEIHWNLIQLAGFFNSWSATQRYKADK
                     GQHPLEIIWPKLTAAWGDENEPRLIRWPLHFRVGRHRIAS"
     gene            complement(12891..>13298)
                     /locus_tag="VFQ23_14645"
     CDS             complement(12891..>13298)
                     /locus_tag="VFQ23_14645"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HET9907887.1"
                     /translation="RFWPTLYQSGFTPRLLLPAEERELLEFGYGITNLVARTTATADE
                     LSPEELVAGQRQLKAKVKKYQPQVVAILGIGAYRTAFAQKVVTLGKQSEPLVNASVWV
                     LPNPSGLNAHYQLADLAKHFQLLREAVEINQSS"
BASE COUNT         3402 a         3249 c         3332 g         3315 t
ORIGIN      
        1 cacgccttct gcggctcctc tttcctcgat tgtcccagct caggctctaa aggagctcgc
       61 ccgcgttgca aacgacgcag aagaaccgat ttatatggtg ttgccaaaag ggcgtggcca
      121 ggtcgtgttc cgtgtcaaag atgtggaaat cgtctcacag ttgatcgacg ggacattccc
      181 cgattatcag cagatcatcc cgcgctcgta taagtctcgt actcttgtat caacttcatc
      241 cttgcttaag gcctgtaagc aggctgagat ctttgcacgc gaaggttcaa atgtggcacg
      301 tttcaatatt aagtctgcgc agggtgaaat gcagcccagc gaagtcgaga tttctgccac
      361 gtcggaagaa acaggtaaaa acgagacgat tgtggaagct actgtggatg gcggggggtt
      421 gctgatcgcg tttaatgtca agttcctgcg tgaagctctg gaggtaatcc gcactccaaa
      481 cgttgcgctg gagacttctg cgccgaacgc gccaggggtg gtcaagccag tcggcgatga
      541 ccagttcctg catgtgatca tgcccatgca tctgggataa aaatatgaat atgtagggag
      601 cggcagctgc ccctccctac gtgtttaatt acaccactgg caacatcact ttgaaggtcg
      661 cgccagtacc caacccttct gactctgacc agatctttcc tccctgtgct tcgatcagtc
      721 cttttgcaat ggtgagccca atccccaacc ctccataatt acgcgtattg ggtggctcga
      781 tctgatagaa ctcttcaaat attttttgta atttatcggt gggaattcca atgccttgat
      841 cctgcaccca tgccaggatt tggttaccct gttttatcgt gccaactgtg atcttgctgc
      901 caggcggcga aaagcggatt gcgttgttca ggacatttcc gagtgccaac gctgttctat
      961 caatatccac atagacaaaa aatggctctt cgggaagtac ataagtcaat ttctggtccc
     1021 gagccgccgc caactctttg atcccttcac aggcataggt gagcacatcc tgcatcacga
     1081 tccgttgagg cttgagattc atctgatccg atttgagcat ggccaggtta ttcatctcat
     1141 ccagaatcga cctcatctgc cccgcggcgt tcagcacctg cgtagcgtgt tcagatagct
     1201 cgcctttggc gtcttccttg aggaaagtgg aataaccaat gatgatccct aaaggtgtgc
     1261 gcaactcatg agaagccaat gccagaaaat tactcttaag ttcgttcgca tccttaactt
     1321 tttccagcgc catctgtgtg gtacgaagga gttgcgcatt attgatagca attgcagcat
     1381 gtgccgcagt caccgatagg atggtcgcgt cgcgttcatc gaacacgcca ttccgtttat
     1441 tgattgcctc cagcactccg acggtccgat ctttgatcgg catgggaacg ccaagcagtg
     1501 tttttacttt aaacttaata tgctctgaaa cgagggcaaa gtgacgcgga tcctgttctg
     1561 cgttgttgat aatgatgggg cgatttgtac ggaagatggt tcctgctagg ctgccctcca
     1621 tgggaaccgg gatctctgcc aattttgccg gatctgaacc tgtagcggca gcgaaataca
     1681 ggcggggatt tttttcgtcg tataaaagaa tggatgcgcc ttcgcaatcc aacagctctg
     1741 tggctgttcc agtgattacc tggagaaggg tttccagatt cagcgttgaa ttaagcgtta
     1801 cgcttaattc aaccaggcgt aagagttgct gttgttgacc ttcgagatca cgaagtctgc
     1861 ctgttgacgt catgaaagta gtgtactgtt tttgggatta acctacaagt agcaaattca
     1921 ttttagaata tcataaatga taaatctgcg atttcggaaa acttcctcca agggcaggtt
     1981 gcaggatgct atctcttcgg gggagatctg ctgattctct tcgacttttc tactcaaaac
     2041 gatatggtct cccaaagaca tgcgactgca ttctgtgctc atgattgcgt cacgatcggt
     2101 cattagtcgc atcgcccgcg gcttgaaaaa gacgatgaca ctgtcggagg atgttttctc
     2161 cctgatgtaa tcatacatct gtaagctgaa aggatcaaaa ggtccgttga tcgagcgact
     2221 attacgtaaa ttcaccgccg cgccaaggct tgaattgacc aggaaaatcc ccgccagcaa
     2281 aatccagaat ccaaggaagg ctttttgagc agtctgatga gatgcttcgg gaagtgactt
     2341 tataaccaat ttcataccct gaaatgtgaa atagatgaaa atggggataa gcgggaagat
     2401 aaagcgcggt ccctgccaga tcggccaggt gataagcatg agcatccaaa gggcaaagaa
     2461 gacaattaaa gcgaaatcgt ctcttcgcct tgaccaaagc ccaatcaaga aaaatatgaa
     2521 gagcagataa tacaaatatt gccagaccct ggattcacca aaaaaggcgc taaacagctg
     2581 aaaataatca attgcgaatt gcagcgcgat ctcaagcttg aatcctctga gttgatcaaa
     2641 ataagattca ctcccgcccg gaaatatcag ggagtacatc agccacaaga ctccgaaact
     2701 catacacacc ataaagatat tctggagaat cttgatgaac agttgtcgat tttttcgatc
     2761 gctccatgct ctgtaagttt caacgattag aaagctggca agcaacagga ttcctaccgt
     2821 acggatgaag aacgcaaaag ctatgagtac tcctattagc acatgatgcg gaatacctcg
     2881 cttgttttcc tttgtcatca atagcaaagc cagagtcgag aaaaacagga aagggatatc
     2941 tgaaagaatc tgatcgagaa aattaatcag caaggggttg aaagcaaaga gagaaacaaa
     3001 gagcaggctt tcgatccgcg tgaaatgatt ccttacaagt agaaacagac agatcgaaaa
     3061 ccccgcaaag aaaattagag ccgggatttt caacgtaagt ggactaatcc ctttaatcat
     3121 ataagcggga gtgagtatca atgggtaacc ccagggataa gcgacgggcc cggtttgaga
     3181 agaagattga tgaatggtaa tgttgttatg ctcaacaaat tcctgcatct ccccatttac
     3241 tatactttct gattgcataa tataggatgc aaaatcatcg ccccattcgt gtccgcgggt
     3301 gaggtgtgac gaaccgatca ccacactgag taagagaatg atgatcaaaa acggattgcg
     3361 gagttcaccc ataaaaccat tttatcgtta ataaaaacag aacatttgtt ctatttttca
     3421 caaataaaat atccgatgtc taagtcggat attttcacac ttctcgcgca gggagtcccc
     3481 tttattgtca caacgacagg ctcaactttg tggcatagtc ctcatgcgac gggcccggcc
     3541 tgttaatgga cgtttagcga tgatggttac tttgccactt ccataaggag tgtacacatt
     3601 gatggcttcc cagccttctt cgccccatgc gtcgagtgtc gcttcgatat cttcatcctt
     3661 tgttccaaac gtactgccaa ttgtttgtac tcgatactcc cattgattga tctcttcaga
     3721 cattttatta atctcctaga gcgaagcaag taatacatgc aggacaatat ctgccgaaaa
     3781 gtgcgcgatc attgcggctt ccagtccacg tttccaatac agccagccaa atatcactcc
     3841 gccaattcca ttcaacagga tggctcgtgt aacgacaagc ggcgtaagcg gaagtaacat
     3901 tgcagtcgct ggaagatgtc caaggccaaa taaaaccgct gcgaagatgt tggcaaccca
     3961 gaataccgta gcggacggtt ttccttccga tgttttgctg atgaaccgtc caagccaggc
     4021 aaaaaaagac atcaccagta atcgcagcaa aatctcttct gcgatacccc cgtagaagga
     4081 tgccagaaat cctttccatg cggcaggatg gatattttga gaatcaagtg cgcttgccga
     4141 atcacctagt tcctttaata acgcaggctg caggaaataa tcgataccaa taatcaacat
     4201 tccggcgagc acaccaagcg caatactgat gggcagaagc gcgcgaattt tgtcagacac
     4261 agattcacgg cgagtcaggg aatccaggat tggagttccc aggccaactc ggcttgcgaa
     4321 gaacattcca gcaacgatca tgatcgcaaa cagaattgcg gcttgtacga tctggagggc
     4381 gagcaacgcg ggaagggata agggcaggtc cattgcttcg agcgctgcgc cttgcaactc
     4441 cagtacgtat ggcaggactg cgatgctgga aacgatggac gcaatccaca ggataaaaaa
     4501 gactcgccag ttaaggatgg ttttcattgg tcaatgtttc acgtgaaaca atcggggtgg
     4561 ggggcacaga ctatacattc gtttcctgct tgtagaggat gtaggaatag atcaccagaa
     4621 atatcgttga gccgaagatc ggaatcatta acatccagaa agccgttgca ccgccaaaga
     4681 aggagccgat gattgcaaat gctccagcca gcatgaacaa ggtcgagccg atccgatgag
     4741 ttttctccca gactcggtca ctcgaaagcg tccatggcgt gcggatgccg ataaagaagt
     4801 tgcgcctggc ttgccgtagc atgaaaccga taaatatgaa gagcaagcca atcgctggca
     4861 acaaggatgt actaattcgg aagcctgtgt atcctacgtt ccacgcaaga gtcagcccgt
     4921 agacgtagag caggaaggcg atgatcagca cgatgaacag gttgaacgcc tcgcgaaatt
     4981 ctgcgatgtt tgccttgagc gggtcaatgg ctggaatgac aaggaacagc accaacatgc
     5041 ccagcgtaat gagaggcatc aggaacacac cccagaaccg gggcatatag ccgtccactt
     5101 cgtcgttgaa attccaatgg gacgccactt gttcgggcaa gcggttccag agcaggagac
     5161 ccgcaattgt ggctccaata agcattacca ggaccaggat ggtcgttgta cgagttgaca
     5221 ttctttctcc tttcttcaac aaaatttact tgttgtcctt tggcaaatta ttctgaccga
     5281 tcgagacgag tccgctcatg gcaccgagat tcatcgtgtc cacggcagaa ctcggcacaa
     5341 ttaccaaggc acctttttcc ttgagtcctt cgaagagcat attcatggca cgcaagtgca
     5401 aagcggtggg attatcaata taagcctgcg atgcctggga aaatgaatcc gcgatctgtt
     5461 tttcagactc accaaggatc acacgggcct gtcgttcacg ttcggcctgt gcctggcggg
     5521 acatggcatc ttccaggtcc tgcgggatga cgatatcgcg tatttcgacg gattgaactg
     5581 ttacacccca gggcgtggtg cgctcatcga tgatttgttg caattctttg tcgattacac
     5641 ttcgtcccac gaggatctct gccagcatgg tacgcccgat aatgtcccgt aaggcggtct
     5701 gggcggccca gtctacagcc gcgcgatagt tttcgacttc cagagcggcc ttttctgcgt
     5761 cccacacgac ccagaataga accgcgtcaa catccacggg aactgtatct ttcgtcagtg
     5821 ttcgctgcgc ggcgaacgga gtgaccatca cgcggtgatc gatccacgct gggattgcgt
     5881 ctatgattgg gataatccaa aataggccgg gaccacgcaa cctgagaaac tttccaagcc
     5941 gcagaacaat cgccttttcc cattgagacg caactttcag cgcgaacaag atataggtgc
     6001 caatcagcga cataccaagt acgtggagtc cgatccaggc tccgtttacg cgtccatcca
     6061 taaggatcgc acccaatacg gcgatggtta gaaaaatgcc aaatattgtc cctgccacac
     6121 cagaaacatg aagatcctta tctttggatg tcttcttttg attcacactt tttataaatt
     6181 ttgaatccat tcggttctcc ttgattactc actatctgac atttgcgatt cttcccggtc
     6241 tttgagctgg cctctgacca tctgaccgat ttctttcttt gagaattcca gccgtagtgc
     6301 ttcaccaata tattttctgg ccagggcttc aagcatttct tttcgcaagc gttccgtcac
     6361 ctctggtgcc gggacttcgg tgatataggt tccgcgtcct tgttgtgtcg agataatacg
     6421 tgcctcatcc agcattcgat aggcccgcgc caccgtgttg aaattcactc tcagttcaga
     6481 ggccagcgcg cggacggttg gcaattgatc gcctggtttc aatgttcctc ctaacacctg
     6541 tccttcaatc tgattcatga tttgaatata aattggaaga ccggaacgaa agtcaagttg
     6601 aagggtcagc ggttcttgtg aaggcatatt gtatctttgt atgattgtac taattgtatc
     6661 attgtcataa ttgtatccaa atggcgcgtt ttgtcaagcc cctcctgaac ggggggcacg
     6721 tatcgcttca ctataagaat tacgtattga gcttaaacaa agctcctgat ggctatcccc
     6781 agccttttaa tgccttcatc aatctgatca ggtgcctgcg caacaaagtt gaggcgtacc
     6841 cagtttatgc ccccaacgcg gtccgggaag aactcgtttc cgggagcgaa gacgacgcct
     6901 tcttccaacg ccacaggcag gagtttctct gaagccaaat catcggggag ctgcaaccag
     6961 agaaacagtc caccctgagg aatatccaga tgaatctcag ctggcaggta ctgcttgacg
     7021 gctgccagca tggcatcgcg acgcttacga aaaatctggc acgaacggcg caggtgggcc
     7081 tgataccgtc caaccgttac atacgcttca agcgcccgtt gcaccagggt cgaagttgca
     7141 aggtcgttga cccgtttgaa attaaccaga gcgtcataga tcggcccctc ggcgaccagg
     7201 aagcccaccc gcaaaccagg catcagtatt tttgaaaatg agttgatata gatgacccgg
     7261 ccgcccgggt ccagggcttt gagggctggt tgagtgcgtc cttcgtagcg caaatccccc
     7321 acaaagtcat cctccaaaat aggaacatta tagcgatccg ccaggacaat caagtcgcgg
     7381 cggcgcggac tgctcaggca cgtaccggtt gggttatgaa aattagggat cgtgtagatc
     7441 agttttgggt ggtgttgctg gagaagtttc tcgagcattt caacctgcat tccctgcgcg
     7501 tcgacgggaa tacccaccac cttgaagccg agcgagcgga atagatcgag ggcacctgaa
     7561 tatgtgggac tttctaccag aattacatct cctggtttta gcagaagctg agataccagg
     7621 gacaaggctt gttgggatcc agcggtgatg aggacgtttt cagcgcgggt ttgcacaccc
     7681 tgactcgtta aaatatgagc aatggtccct cgcaatggag cgtgtccgtt cggctctcca
     7741 taatccagag cggcaattcc atcgcgccgc atgacggtct gaagcacctt acgaaattcc
     7801 tcagcgggaa aaagatgcgc atctccaatt ccgctatcga agtggatcgg gtcacgatga
     7861 ctcgttttat gcctggaagc cttattctgt ggtttgtttt tgctggattt atcctgaagc
     7921 tctacgctct gctgccagag aggccaggta gcattcggac catctttgga aagtgtcaac
     7981 agcgaattgg tcggcagaac gtacgttccg ctgcccatcc tcgaataaat caacccttcg
     8041 gtctcaagtt cggcgtacgc gttttcaact gtgatgcgat taacacccag gtcacgtgca
     8101 agctgacggc tggcaggcaa acgcgtgtct gcaggcaggc tgcctgaaat gattccctgt
     8161 cgtaaatacg tttcaatctg ttgataaaga gggattgagc tttgtcgatc cagtggaatg
     8221 cgcatggtac acctgagtaa agaattggta tggctggtat aacataattt gaagaatttc
     8281 catagagcca atttgccttc gattatcata atcgaataaa ttggtctata attttttcgt
     8341 gaatattggc tcttaactta tgccaatttt ctcctagaat ataggtatct gaaacatgtc
     8401 cattctgacg tttggatgta cgctctcaat ttattagtaa tcaggagtaa tacaatggca
     8461 actatggaac aacaaacggg tacctttgct gtaaagaaag gcttggctca aatgctgaaa
     8521 ggcggcgtca tcatggatgt ggtgacagcc gagcatgcca agatcgcaga agacgccggc
     8581 gcctgtgccg tgatggccct cgaacgcgtc cctgcagata ttcgtgccaa cggcggcgtg
     8641 gcgcgcatgt ctgacccgga cttgatcttg aagatcatgg attcggtcac tattccggtg
     8701 atggcgaaat gccgtattgg tcactttgtc gaagcgcaaa tactcgaggc gatcggggta
     8761 gattacatcg atgaatcaga agtactgacg cccgctgatg aagcaaatca catactcaaa
     8821 cataacttta ctgtcccgtt tgtgtgtgga tgtcgcaatc tcggcgaagc tttacggcga
     8881 gtcggtgaag gcgcggcaat gatccgcacc aagggagaag ccggaacagg ggatgtcgtc
     8941 gaagcggttc gtcacgcgcg gacggtgctt ggggatatcc gtcgactgac cacaatggct
     9001 gatgaagagt tgatgagtta tgcaaaagag atcggcgcgc cctatgaact catcaaagag
     9061 acaaaggaac tcgggcgcat gcctgtggtc aactttgcgg ctggtggagt cgcaactccg
     9121 gccgacgcgg cgttgatgat gcagcttggt gtagatggcg tgttcgttgg ctccgggatc
     9181 ttcaagagtg gcgatccttc tcggcgtgcg gcagctattg tcaaagctgt gactcattat
     9241 caggatgcaa acattcttgc ggatgtgagc aaggatctgg gcgagccaat ggttggacgc
     9301 aatgtgtcac aaatgcctga gtcggagaag atcgcacaac gcgggtggta aatttatcgt
     9361 aggggcacag caaaacataa agcggtttta cacattgatc gtcccgctgt gcccttacag
     9421 gatcaaacat gaaaatcggt gtactcgctt tacagggcga ttttgctgag catatcgcca
     9481 tgctcaaaaa tattaatgca gatgcggtgg aagtccgttt acctgatcag ctcaaaggat
     9541 tggatggcct gattattccc ggtggtgaat cgacgacgat cgggaagctc gctgtggctt
     9601 atcacctgat ggaaccgttg aaagaatttg ggcagcgcca cgcgatctgg ggaacctgcg
     9661 cgggcgcgat ctttctttct aaggatgtga gccgtgacca gcctctactg ggactcatgg
     9721 acatcaaagt cgaacggaat gccttcggcc gccaggtgga ttcctttgaa gcagacctgg
     9781 atgtgcccga acttaaacaa gccactggaa cagacgaaga ttatcacgcg gtcttcatcc
     9841 gcgctccgat cattgagtcg gtaagcggtg atgcgaagat tctcgcctct gcaccggacg
     9901 ggaggattgt cgctgcacaa cagggacatc tcctggctac ttcgtttcac cccgaattga
     9961 caaacgacac gcgcttccac cagtattttc tgtcgctggt ggattagcat tacctgtcat
    10021 tgcgagccgt cgtttgagcg aagcgaaggc ggcgtggcaa tctcaactga ttaataaact
    10081 tttgtttaga tgttacttga gattgttgcg ttgctcccgc gaagcgagtc acatctcgca
    10141 atgaaatctc taaaaaggaa tcaatatgca ctacaaactt gccttcatcg gattcggtaa
    10201 tgttgcccgt tctttggttc gattgctcga acgaaagcgt gatctgctaa agcgagaata
    10261 cgatattaca tattccatta ccggcatcgc cacggggcga catggttttg cagtcaatcc
    10321 tgatggaatc gatgaacaac aagctctggc tttagtagaa agtggaaagt ctatcaatcc
    10381 actgtccact ttccaggtgg acgattctct tgccgtaatt caacacgcac aagccaatgt
    10441 catgttcgaa aactccccgg tgaattacga gacgggtcag ccagcgattg accatacgaa
    10501 agcggctttg aatgcaggta tgcacgtcag cactgcgaac aaagggacgg ttgttcacgc
    10561 ctatcaggaa ttaacttcac ttgccaaatc caaaggcaag aaattcatgt tcgagtcgac
    10621 ggtgttgggt ggcacgcctg tattttcaac ctttcgcgaa gccatgcctc ttgcagaatt
    10681 gatgtccttt aaaggcatta tcaatgcgac cacaaacgtt attctttcaa gaatggagga
    10741 tggcgagtca tttgaggatg ccgtgaaata ttgtcagagc attggcattg cagagacaga
    10801 tccctccggt gatgtagatg gctgggacgc gtcgatcaaa gtggcggcat tagcgactgt
    10861 gttgatgaat accccactca aaccgcagga tgtggagaga aaaggcattc gggagatcac
    10921 gcctgagatg gtgaatgaag cgaaggcgaa tggaaggcgc tggaagttgg tcgcatcggc
    10981 tgagagagtg gggagtcaga tccgagcgcg cgtcgccccc gaattggttg acgcctcctc
    11041 gccgttgtat ggcatgatgg gttcatcaac cgggctaacg tttcggacgg atgtattgcc
    11101 tgattactcg ataaccgtct ctgaacgcga aggcatgaaa ggcggaccgg aagagaccgc
    11161 ttatggactc tttgccgatt ttgtcaacgc agtgaaatca taaataagct gcaatagggg
    11221 aatataaacg aaggggagcg acggtgataa agcccaatcg taaatagagc ttttctgcaa
    11281 gcggtgatga gtcaagcacc gccagtttgt gtcccatctc tttggctgcc ttcatcgcga
    11341 cgttcatcaa aatacttgca atcccaagcc cgcgcgcctc aggcttggtg gcaacaccgt
    11401 agattcctgc cgagcctgaa ccgttataca gaccgatctt tgaaatcgga acaccgtcct
    11461 tcaatgccag ccacatgcgc gtgttcgaat tgcgctctcc aatttcaaat ctttcgatca
    11521 tctttctcaa ttggaggcga tattcctccg ggactcccca gcgccaggcc gcgagcccat
    11581 acagttcgat caggtcactt tcttcgatcg cttcgcgaat ctccacccct ggcggcacgg
    11641 gcggcgcttc aggaagatcg tccaggccgc gcgccatgca cggggcaatt tcaatttcct
    11701 gcagaccatg ttcgaccaag cgttgaggca ggtcggtcgg taaggccgtt ggatgcacga
    11761 tccaaagctg tgtgacattt cgatcagcat agcctttgac cagtgagcca atccgttgat
    11821 cgatatcctt ttccacttga aatcgcaaga cggtgttgta cggcatggtg ggaatgggtg
    11881 tctcgaacca gagcgcgtcg tcctcgtcgt ggagggtaca gcctgggcca cgtccgaagt
    11941 tagcccaagt ctcccaaaga tttagttcca cctgatgaat cacttcctct tttgaatatc
    12001 ccggtattcc ctcaacttcg cgcatgaatg cttctccgca taaaagattg gcgaatttta
    12061 cacgttaatt cctcagagaa atcaagaatg caatgaattg cttaggaagc gatgcgatgt
    12121 cgcccaactc gaaagtgcag gggccaacgg atcaggcgcg gctcattttc atcgccccag
    12181 gcagccgtca gttttggcca aatgatctcc agtggatgct gtcccttatc ggctttatat
    12241 cgttgcgtgg cagaccagct gttgaaaaag ccagccagtt ggatcagatt ccaatggatt
    12301 tccattacaa atgctggacg cacgatctcc tcaaatggga agggaagggt ttcatacttt
    12361 tcttcaagat aacgaatccg ttccggccaa tatccatcta aaatcccttt gtaatattct
    12421 tcggtcagtt gatcgatctc gggagtaatt tcagtcacgt tgtaggtcca ggcggcaatg
    12481 acaccattgg gcttgagtac gcgtttgact tcgcgataga actcgtcaaa gttgaaccag
    12541 tgaatggcca ccgcgactgt gaccaggtcc gtgctggagt cgttgagcga gacgtgttct
    12601 gcaggttcca cacgatattc aactttctct ttctgatagg cacgcgaaat ttgttctgca
    12661 ctggcgtctg tcgcatgaac tttgtcgaaa tgttttgcaa gcccaatcgc ggcctgaccg
    12721 ttaccggttc cacagtccca ggcaagtgac aggcccggag aaacggaagc aaggtaagca
    12781 taaatttcat ccggatattg tgggcgatac tgtgcatacg tctcagcctg tttcgaaaaa
    12841 tggtcttcaa agttaaaggc gttatttctg tctgacatag cgggttcctt ctaggatgat
    12901 tgatttattt ctactgcctc gcgtagcaac tggaaatgct tggctaaatc tgccagttga
    12961 taatgagcgt tcagtccgct ggggttgggc aatacccaaa cagatgcatt caccagcggt
    13021 tcggattgtt taccaagcgt aactactttc tgagcgaaag ccgttcgata ggcaccgata
    13081 cctaagattg ctacgacttg tggttgatac ttcttcactt tagctttcag ctgcctttgc
    13141 cctgctacca gctcttccgg acttaactcg tctgctgtag cagttgtgcg cgcaacaaga
    13201 ttcgtgatcc cgtatccgaa ctccagcagt tcgcgttcct ctgctggtaa cagcagacga
    13261 ggcgtaaaac cagactgata cagcgtgggc caaaaacg
//
LOCUS       DASTJP010000502        19287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_696079, whole genome shotgun sequence.
ACCESSION   DASTJP010000502 DASTJP010000000
VERSION     DASTJP010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696079"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFQ23_14650"
     CDS             complement(<1..636)
                     /locus_tag="VFQ23_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9907888.1"
                     /translation="MNKRFHRATLLILLITVMAACASPIIQPSSSEEVATGLPQTSEA
                     ATIEPGDMPEASSNLLPRSLYFLGIDGGGTRQVYRMERDGKMITQLTFESVDVSAYDV
                     ALLDGRIAYGAGNQLILSDANGSNRQVLVDGRAGPDAQGFSSLAFSPNGKTLAYASEG
                     LNLYDVSTGVSSLAPQSAFARPVKFSPDSTKLLVTVSPPNTDAAHDEIYFPA"
     gene            complement(777..2006)
                     /locus_tag="VFQ23_14655"
     CDS             complement(777..2006)
                     /locus_tag="VFQ23_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907889.1"
                     /translation="MNRRFPFLFTLLILAVLIAACNVPGTNTTQPASGDPLATAVVQT
                     LQALTPVVETGETPEPEAPADGLLPHTLYYLGTDAAGLKQVFRLERDGTTQRQLTSEP
                     VDVTSFGASPVDGSVAYVANNQLQLINADGSDRRMLVDGGVMDENNPFLNVINNPVFS
                     PNGQTLAYGYKGLNLYALSSGVSNLVIENQLDDSTGSGFIFPRELYWPERYSPDGSKL
                     LITLGYYEGASAAIFDPASGGLVRLTGGEGALICCDETEWSADGSSIYSASRSAGMFT
                     SGMWKVDPSTGVVTTLFTSNYDTSDFNYADEPYLGPDGQLYFFYAKANTEINSRTPLQ
                     LVRSAPDGITGRTVLRPETFETMNEVLWAPDASFAVVALANTPDTYQGGRAEIVYIDG
                     RPNVVLVPFAQQMRWGP"
     gene            complement(2044..2307)
                     /locus_tag="VFQ23_14660"
     CDS             complement(2044..2307)
                     /locus_tag="VFQ23_14660"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HET9907890.1"
                     /translation="MKMIILIVKDTDADTITQELTSAQYRVTRVASTGGFLRKGIVTL
                     LLGTRDERVEAALELIRAKLTPLPSGEKRVTLFVVPVERFEQV"
     gene            complement(2368..2706)
                     /locus_tag="VFQ23_14665"
     CDS             complement(2368..2706)
                     /locus_tag="VFQ23_14665"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HET9907891.1"
                     /translation="MDTHSIKLLMLAVVQEQDRDTASHALEHLKLPVVYLFSSGGFLG
                     RRNATLLIGLREGHVEEAIQTLEESCRQRVEYLTLPLEGSPLPMPTPVPVTVGGATVF
                     TLPVELFEEI"
     gene            complement(2791..4596)
                     /locus_tag="VFQ23_14670"
     CDS             complement(2791..4596)
                     /locus_tag="VFQ23_14670"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HET9907892.1"
                     /translation="MRKPAEFIPINTVLIPLVYEGPGTQALELALCLDANITLVGVVV
                     VPSHESLSVGAVSARELRRQLRQYGKEPRITSKAQIIVSYEPWQELSKLLQDEQPDLL
                     CLEWDAHFDALGVSVSEVLTRPPCNVALMRGKLINPPKQVLVPVRGGPHAELALRVGL
                     GLQSKGVTALHLRHPDDPKAGSDAPFKGLERVLKQMPEVQKEFAVTNDAAGLILEKAK
                     QADAIVLGTTAQPLTSSVSLGPFADQILRDAPGAVIAVKSARPLPQAEFDESSGAQAI
                     SILVDKWFAENTFHADEFTQLDELVRLKQEQGLTISAALPALNEEESVGNVIDTIRDA
                     LMKEVPLLDEMVLIDSNSTDRTREIAEERGIPVYIHQEILPQYGARPGKGEALWKSLY
                     CTTGDILIWIDTDIVNIDPRFVYGLIAPLLHRPEIHFVKGFYRRPLKVGNKIQAGGGG
                     RVTELTARPLLNLFYPELSGIIQPLSGEYGGRRTALERLPFFSGYGVEIGLLIDLFET
                     FGLSAIGQVDLRERVHHNQTLEALSKMSFAIIQAVIRKLEKRYDQKFLENVNKTMKLI
                     RYERERFFLDVDEIAEGERKPMLEVEEYKIKFGKL"
     gene            complement(4677..5897)
                     /locus_tag="VFQ23_14675"
     CDS             complement(4677..5897)
                     /locus_tag="VFQ23_14675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9907893.1"
                     /translation="MNIAVLHYSVPPVVGGVESVIAHHARLMTKGGHAVRLIAGRGSA
                     LGDDIPLVSLPLVDSQDPRVLAVKEQLAGGDVTEDFDSLRDELATQLRNALEDVHVLI
                     VHNACSLNKNLALTAALYKLHAERQLPGLILWHHDLAWTTPRYLPELHDGYPWDLLRT
                     DWGNSRHVVVSELRRRELTELMKIDPPSVYVVPNGVDVARFYKLQLQTQTLLEQTKLL
                     DAAPILLLPVRITRRKNIELALETLSKLRNSFPQAALVVTGPLGPHNPLNREYFDELK
                     MLRTQLGLQDSVYFLAELSDSFLPDEVIADFFRIADALIFPSREEGFGIPLIEAAFSH
                     LPVFCADIPPLHELGLDDAYYFSPDEDPAILANAIASYFQSNLPARLAMRARTLFRWE
                     AIYEEHIAPLLNDS"
     gene            complement(5894..7237)
                     /locus_tag="VFQ23_14680"
     CDS             complement(5894..7237)
                     /locus_tag="VFQ23_14680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9907894.1"
                     /translation="MKPRSHHIGFISTRFAGTDGVSLETEKWATVLQQLGHDCFYFAG
                     ECDRPAERSLVVPEAFYRHPEIDAINVQAYSGSWLVTKAAREAHPEIAKLYKDFFSIY
                     IRPPAMTRRVNELKEYFKVKLYEFAHRFELEILIVENALTIPLNLPLGLAITEFVAET
                     GFPTIAHHHDFHWERQRFHVNCVGDYLAAAFPPVLPSIRHVVINSIQAQQIASRHGVT
                     SRIIPNVMDFDSPPPDLDPYTESVRADFGIGPGEYFFLQPTRVIQRKGIEHAIELVHR
                     LGLPAKLVISHAAGDEGSDYEQRVREYAQLMDVTVRFESDQVQDERGLTEDGRKIYTL
                     GDVYPHADLVTYPSSIEGFGNAFLEAVYYHRLILVNNYSIYEVDIKPKGFRTVWFDGF
                     ISRNTLDMVREVLQNPERAKEWADENYQLAKRYFSYTVLEHRLQSILADCLGYQV"
     gene            complement(7234..8916)
                     /locus_tag="VFQ23_14685"
     CDS             complement(7234..8916)
                     /locus_tag="VFQ23_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HET9907895.1"
                     /translation="MTDLLSQLTFLYGADAAPHVLTGVQNILAAYVDRIPVRNGALTE
                     KDSILITYGDQVQTSSEKPLQTLSAFCKRYLKDVVGGVHFLPFFPWTSDDGFSVVDYR
                     KIDPALGDWEHVSAMQSDFRLMFDAVINHISSQSDWFQAFLKDDPHYRDYFVVVNGSP
                     DLSQVVRPRALPLLTSFTTPSGEKRVWTTFSDDQIDLNVQNPEVMMEILDILLMYAER
                     GATFIRLDAIGYLWKEIGTSCIHLPQTHGVIQFLRAALNEVAPHVHLITETNVPHVDN
                     ISYFGDGTNEAQLVYNFALPPLTLHAFHTGNARVLSDWAKTLTLPSDKTTFFNFLASH
                     DGIGLNPARGILSNADIDSLVHKTLTHSGLVSYKHNSDGTQSPYEMNINYFDALSDPL
                     GTEPLRLQIDRFMTSQAIMLSIVGVPGIYFHSLFGSRGWLDGVGQTGRNRTVNREKCD
                     LQSLQRELEDPQSLRGQVFAHYRELLRARSSSAAFHPHGRQKILDVHPSVFALERISP
                     DGTSRALCLHNVSNKEVAFSTTYESGIDLLTNQPVKSAQITLNPFQISWMKL"
     gene            complement(9178..9825)
                     /gene="ruvA"
                     /locus_tag="VFQ23_14690"
     CDS             complement(9178..9825)
                     /gene="ruvA"
                     /locus_tag="VFQ23_14690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET9907896.1"
                     /translation="MPFAASAQGYSTSIIYCGKNAFMIATLRGEISQIEETAIILEVG
                     GVGLRVFVPAPVRTRAKAGESVLLFTHLVVREDSLTLYGFESQAERELYNTLLGVDGV
                     GPKVALAVLSTLTVDAIQRAVFADESEVLNRVPGVGKKTAQKMALHLKDKLKPTDALA
                     GVAAMADYDSEVLAALTALGYSVVEAQSAIQSLPRDAPTDVEERLRVALQYFTGR"
     gene            complement(9906..10388)
                     /gene="ruvC"
                     /locus_tag="VFQ23_14695"
     CDS             complement(9906..10388)
                     /gene="ruvC"
                     /locus_tag="VFQ23_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET9907897.1"
                     /translation="MTLALGIDPGTATTGYGLVRMEPDGTLVAVKYGVILTPKDATAS
                     ARLVMLYNELQELLHEYKPQLAAVEKLFFARNVTTGIAVGQARGVVMLAIEQAGVAVF
                     EYTPKEVKNAVAGYGSADKRQVQEMVRALLQLDSIPKPDDAADALAIAITHLNTKRYD
                     "
     gene            complement(10439..11194)
                     /locus_tag="VFQ23_14700"
     CDS             complement(10439..11194)
                     /locus_tag="VFQ23_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9907898.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGAIFTRLAREIVLSARDGGGDPD
                     SNFRLRLAVDKARAENMPKDNIERAIRRGTGEDKDSAAFEQITYEGYAPHGVAVMVET
                     VTDNRNRTISDLRHAFSKAGGNMAELGAVGWQFDRISYFSFPSSAMNFDKAFELGIEA
                     GANDVIEEEGHIEIIAPVESFKIIADALHKANVQPEEAGLRMSPKQEMELGPEDSVQV
                     LKALESIEDLDDVQNVYSNLKVSDEAIAALEAA"
     gene            complement(11299..12585)
                     /locus_tag="VFQ23_14705"
     CDS             complement(11299..12585)
                     /locus_tag="VFQ23_14705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9907899.1"
                     /translation="MSADHSRYRWFVVAVFFFFMLLHQTDKLLIGPLESQIRDSFQIT
                     KTQMGAVLTGSLIVGTIFYPLWGYLYDRYARSKLLALASFIWGATTWLSSVVRTFPQF
                     VATRASTGIDDSSYPGIFTLVADYFGPNLRGKVYGLLQLSQPIGYLFGTVLALMIAPG
                     LGWDWRTIYIATGSLGIVLAILIFFGIKEMPRGKAEPEFENMAEMTSFHFSWEEARQI
                     FKKRTMWFVFLQGFAGVFPWNVIVYWFFTYLEEERGYDQNSILFTMAPVILVLAAGYF
                     VGGALGDYLFKRTNKGRILISCVGVLLGAIFLFLAINTPVEARNQFFILMVITAIFIP
                     LSSANVIATVYDVTVPEVRSTAQAVEYFIENTGAAFAPFLAGIIADATNLKTSILLIC
                     VTAWILCFCFYLGALFTIDKDTNNLRNQMAERAKAM"
     gene            complement(12697..12846)
                     /locus_tag="VFQ23_14710"
     CDS             complement(12697..12846)
                     /locus_tag="VFQ23_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907900.1"
                     /translation="MRGLKWILLGIYLVIVGLSLLGVSLGGNVLNLIAGICALIAGIL
                     FIINR"
     gene            complement(12925..14157)
                     /locus_tag="VFQ23_14715"
     CDS             complement(12925..14157)
                     /locus_tag="VFQ23_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HET9907901.1"
                     /translation="MKILHFADAHIDMANYGRHDPETGLPLRVLDFLKSLDTIIDAAI
                     SEQVDMVIFAGDAYKDRSPAPTFQREWEKRIVRLSQAKIPTLLLVGNHDLSPAIGRAH
                     AIQEFDTLQVPYIRVLQKPEFLLSKDLWGLPVQVIAMPWVSRSGLMAAMASSATETSE
                     IFSKIENRISELVEDWIKEADPGLPLILTAHASVEGAVFGAERTVMLGSDLVLPVSLV
                     KDKRLDYVAMGHIHKWQDVNRGEHPPVIYPGSIERIDFGEAHDDKFFVIAEITRGYST
                     VEWRKLTGRQFVDKWIVLESNENVTDVLKAALPDPRNMANAIVRLTVEYPREMDVLID
                     ESALREYTVGAFEFHLAKKPKVEARVRLPEGQTVGSLSPLELLDQYWRATKTGDSEYL
                     QQLAQEIISDETFGTANP"
     gene            complement(14308..15657)
                     /locus_tag="VFQ23_14720"
     CDS             complement(14308..15657)
                     /locus_tag="VFQ23_14720"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET9907902.1"
                     /translation="MQNRFNRKWVIPIVILAGIGLFFLPPVYSRAMPRIDGWMADIKY
                     RINPPDQAVFQPAQQAQIDLAVTEMMQTAIASLTPEVPTVTTTPTRGPTLTPTLSPTP
                     LPASVKLAGFRYEDQKNRWNYCGPANLSMALNFVGWDGDRDDVARAIKPGVPDPKLDF
                     IQQGRSDVNVMPFEMVDFVNDETEFRALSRLGGDLELAKSLLAAGFPFIAEKGYYEKD
                     YTGRVAWLGHYQFVTGYDDARQELTVQDTWNDGPNFRIPYDEFMASEAWLSFSNLFIV
                     VYPPEQEAKLLATLGPYADANWAARQALSIAEENIRGTNGINLYFAWFAKGSSHVALQ
                     EYADAAAAYDQAFSIYDSIGEDDKQRPYRMMWYQTGPYWAYYYSARYQDVVNLANITL
                     TETISKPTLEESIYWRGRAKYMIGNTQDAVDDFREALRLHPNWPPAIQALQDLGFQP"
     gene            complement(15765..17066)
                     /locus_tag="VFQ23_14725"
     CDS             complement(15765..17066)
                     /locus_tag="VFQ23_14725"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET9907903.1"
                     /translation="MQNRYSRIWAIPVFILLIIGIYYIPPVHSRLAWRLEFVRTQIKY
                     FLNPPEEAVFQPQQQSPAEIAATQTMQSPKTQTPIPSPTVRPTITTTPLPASVLLENV
                     KYEQQHGRRNYCGPANFSMALTYWGWQGDRDVIGKVVMPINEKDKNVMPYELQDYITE
                     NVPGMTSIIRNGGDIATLKRLVSAGFPVIVEIGVYEVDINGKNSWLGHYGFVTGYDDA
                     NQMIIYQDSYQPEPTTNPGPNREISYEKLTQRWRAFNYVFVVVYPIEKQNEVLTLLGP
                     LADEEQANRHALEVARAESQTLTDVDEYFAWFNAGTSHVALFEYTDAAIAYDRAFNIY
                     ANLQVDSSIRPYRMMWFQTGPYKAYYYTDRFSDVISLADTTLNDTIAEPVLEESLYWR
                     GMAYYMAGETDLAVRDYRAALKVHPKWSPATQALQDLGIQP"
     gene            complement(17137..19080)
                     /gene="dnaK"
                     /locus_tag="VFQ23_14730"
     CDS             complement(17137..19080)
                     /gene="dnaK"
                     /locus_tag="VFQ23_14730"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET9907904.1"
                     /translation="MRKQIEISARTNRSISMSKIIGIDLGTTNSVVAVMEGGSPTVIS
                     TAEGGRLAPSVVAFNKNGERLVGQTAKRQAVVNPENTIYSIKRFIGRHFGETEAERKM
                     VSYDVVQGPSDDVRVKIPVTNREYSPQEISAMVLGKLKADAEAYLGQAVTQAVITVPA
                     YFNDSQRQATKDAGKIAGLEVLRIINEPTASALAYGLDKKKNETILVFDLGGGTFDVS
                     ILEVGEGVIEVKATNGDTHLGGDDWDQRIVNWAADEFKKDQGIDLRSDRQALQRLREA
                     AEKAKIELSTVMETEINLPYITADATGPKHLQLKLSRAKFEQMTEDLLERCRKPFEAA
                     LKDAGINAGKLNEVVLVGGSTRMPMVQDLVRKLTNGKEPNKGVNPDEVVAIGAAIQGG
                     VLGGDVKDILLLDVTPLSLGVETLGSVMTKMIERNTTIPVRKTETYSTAADNQTAVDI
                     HVLQGERSMAADNMSLGRFRLDGIPPAPRGIPQVEVTFDIDANGILNVTAKDKASGKE
                     QKVTITASTNLDKSDIDRMVQEARSHEADDKKRRELIDAKNTADGLVYQTEKALRDLG
                     DKVPSAERGEIETKINDLKTAAQGDDLSRIQQASEAVQQAFHALSQQLYAQGQPEPEA
                     AGGPSTPPSGSGDDDVIEGEVKE"
BASE COUNT         4869 a         4981 c         4748 g         4689 t
ORIGIN      
        1 cgcaggaaag taaatttcgt catgggcagc gtcagtgttt ggagggctaa ccgttacaag
       61 aagctttgta ctgtccggtg aaaattttac aggtcgagca aatgcagatt gcggcgctag
      121 actggaaaca cctgtggaaa catcataaag gttcaagcct tcactggcat aggccagggt
      181 ctttccattt ggggaaaatg cgaggctgga aaacccttgc gcatctggtc cggcgcggcc
      241 atccaccaaa acctggcggt ttgagccatt tgcatctgac aggatcagtt ggttccctgc
      301 tccataggca atacgtccat ccaacaacgc cacatcatag gctgatacat caacagattc
      361 aaatgtaagc tgggtgatca ttttgccatc gcgttccatg cgatagactt gtctggtacc
      421 gccaccgtcg atgccaagaa agtacaagct gcgcggaagc aaattcgacg aggcttcagg
      481 catatccccg ggctcgatcg tggcagcctc ggatgtctgg ggaagacccg tcgctacctc
      541 ttcagaggaa gacggctgga ttattggaga ggcacaggct gccatgacag ttatcaagag
      601 aatcaatagc gtggcgcggt gaaaacgttt gttcatagaa acctcatatc gacaagaatt
      661 acttcaaaat caattattgt gatcgactga gtttctaaaa cgcaaccgtg actaattttg
      721 ttccaactaa atttgtgaaa tcgtagggaa cggcggccgc cgttccctac attaatttat
      781 ggtccccatc tcatctgctg ggcaaacgga acgagtacta cattcggtct gccatcgata
      841 tatacgattt ctgctcttcc accctggtag gtatctggcg tattcgcaag agctaccacg
      901 gcaaaacttg catctggcgc ccagagcact tcgttcatgg tctcgaaggt ctctggtcgg
      961 agcacagttc gtccggtgat gccatctggt gcagagcgga caagttgcaa tggcgtgcgg
     1021 ctgttgattt cagtgttggc ttttgcatag aagaaatata actgaccatc gggtccgaga
     1081 tagggttcat cagcataatt aaaatcgctc gtatcataat tactggtaaa gagcgtagtt
     1141 accacacccg tagatggatc cactttccac attccagaag tgaacattcc ggcgctgcga
     1201 ctggctgaat aaatggagga gccatccgct gaccattccg tttcgtcgca gcagatcaga
     1261 gccccttcgc caccggtcag ccgaaccagc cctccgctcg ccgggtcaaa tatcgcagca
     1321 gacgcacctt cgtaatatcc aagtgtgatc agcaatttgc ttccatcggg ggagtaacgc
     1381 tccggccaat ataactctcg cgggaaaata aatccactgc cagtcgagtc atcaagctga
     1441 ttttcgataa cgagattaga aacgccgctc gaaagggcat acagattcaa gcctttataa
     1501 ccatacgcaa gggtctgccc attcggggaa aaaacaggat tatttatcac attcaggaac
     1561 ggattgttct catccatcac ccctccatcc accagcatac ggcgatctga tccatcggca
     1621 ttgatcaatt ggagttgatt gtttgcgaca tacgcaacac taccatctac aggtgaggca
     1681 ccgaaactgg ttacgtccac tggctcagag gtcagttgcc tttgggtcgt tccgtcgcgc
     1741 tccaggcgaa agacctgttt caggcctgcc gcatccgtac cgagataata taaagtatga
     1801 gggagcaatc cgtccgcggg cgcttcgggc tcaggagttt cgcccgtctc aacaacgggt
     1861 gtgagcgcct gcagggtctg aacaacagcc gtggcgagcg gatcaccgga cgcaggctgg
     1921 gttgtatttg tgccaggaac attacaggcg gcgatcagaa ctgccagaat caacagagta
     1981 aaaaggaatg gaaaacgtct attcatgaat atgcagacta gtctggcgtc aattttgttc
     2041 cgcttatacc tgttcgaatc gttccacggg caccacaaac agggtgaccc gtttttcgcc
     2101 agagggcaaa ggggttagtt tggcgcggat caactccagc gcagcctcaa cgcgctcatc
     2161 gcgtgtaccg agtagaagag tcacaatgcc ctttcgtaag aatcccccgg tggaggcgac
     2221 gcgagtgacg cgatattgag cagaggtcag ttcctgggtg atggtatcgg cgtcggtgtc
     2281 tttgacgatc aatatgatca ttttcatgat gtcatctcct catgtaaaag accctaacgg
     2341 tcttatagac cgttagggtc tctaaaatta gatttcctcg aagagttcca caggtaacgt
     2401 gaaaacagtc gcgccgccca cggtaacggg aacgggcgtc ggcatgggaa gaggggatcc
     2461 ctccaggggc agggtgagat attccacccg ttgacgacag gattcctcca gagtctggat
     2521 tgcttcctcc acatgtcctt cgcgcagccc gatcagcagc gtggcgttgc gtctgcccag
     2581 gaagccaccc gaactgaaca agtagacgac tggcagcttc agatgctcca acgcgtgtga
     2641 tgcagtatca cgatcctgct cctgaaccac ggcaagcatc agcaatttga ttgaatgagt
     2701 atccatggca acctcactaa atggattaaa accatctacg gaatagtata caggatactg
     2761 ttaaatgcac aagacattta tggaatttat ttagagtttc ccaaacttta ttttgtattc
     2821 ttccacttcg agcatgggct tgcgttcccc ttctgcaatt tcgtccacat ccagaaagaa
     2881 tcgttcacgt tcataccgaa tgagtttcat cgtcttgttc acgttttcca gaaacttttg
     2941 gtcgtatcgt ttctccagtt ttcttatcac cgcctggatg attgcaaacg acattttgct
     3001 cagcgcctct aacgtttgat tgtggtgaac gcgctcccgc aaatccacct gtccgatcgc
     3061 gctcaaaccg aacgtttcaa acagatcgat caatagcccg atttccacac cataccccga
     3121 aaagaacggc aggcgctcca gtgctgttcg ccgcccacca tattcacctg atagcggctg
     3181 aatgattccg gaaagttcag gataaaaaag attaagcaat gggcgcgcgg ttagctctgt
     3241 gacccggccg ccaccgcctg cctgtatttt gttcccgacc ttcaatggac ggcggtaaaa
     3301 gcccttaaca aaatggattt caggtctgtg caataaagga gcgatcaaac catagacgaa
     3361 acgcggatca atatttacga tatccgtatc gatccagatg aggatgtcgc ctgttgtgca
     3421 atacaggctc ttccacaggg cttccccttt acccggacgt gcgccatatt gtggaagtat
     3481 ctcctggtga atatacacgg gaataccacg ttcttctgcg atctcgcggg tccgatcggt
     3541 tgaattcgaa tcgatcaaaa ccatttcgtc caataaggga acttctttca ttaacgcatc
     3601 tctgatcgtg tcaatcacat tccccacaga ctcttcctca ttcaaagcag gtagagcagc
     3661 gctgatcgtc aaaccctgct cttgcttgag tctcacgagt tcatccagtt gagtaaattc
     3721 atctgcatga aatgtatttt ccgcaaacca tttatcgaca aggatggaaa tcgcttgtgc
     3781 acccgaactt tcatcaaact ccgcctgagg tagcgggcgg gcgcttttca ccgcaatgac
     3841 cgcgccgggt gcatcacgta ggatttggtc cgcaaatgga cccagcgata ctgaactggt
     3901 gagaggctgg gcagttgtac ccagcacgat cgcatcggct tgttttgcct tttcaaggat
     3961 caagcctgcc gcatcattcg tgactgcaaa ctccttctga acctcgggca tttgcttcag
     4021 aacgcgttcg agccctttga acggcgcatc gcttcccgcc ttagggtcat cagggtgacg
     4081 cagatgaaga gccgtcacgc ctttggactg cagacccaga ccgacgcgta acgccagttc
     4141 cgcgtgcgga cctcctcgta ctggaactaa aacctgtttc ggcggattga tcagtttccc
     4201 gcgcatcaaa gcgacgttac atggaggacg cgtcaatact tcgctgacac ttacgcccaa
     4261 cgcgtcgaag tgcgcatccc actccaaaca taacaagtcg ggttgttcat cctgaaggag
     4321 ttttgacagt tcctgccagg gttcgtacga gacaatgatc tgtgctttgc tggtgatacg
     4381 cggctctttt ccatactgac gtaattgcct gcgcaattcg cgcgcactaa ctgcccccac
     4441 actaagcgac tcgtgagacg gcacaaccac cacgcctacc agcgtaatat ttgcatccag
     4501 gcagagcgcc aattccaggg cctgtgtgcc aggaccttca tatacaagag gaatcaaaac
     4561 cgtattgatc gggataaatt ctgcaggctt tctcatgaat tattttcctt gactcatcaa
     4621 atcttgccgt gatgagccgc aatttcggca tgccaaagtg aagaaaatga gtcgcgctat
     4681 gaatcgttca acagcggtgc gatatgttcc tcgtaaatag cctcccaacg gaacagggtg
     4741 cgggcacgca tggcaaggcg cgcgggcaaa ttggattgaa aatagctcgc aatcgcattc
     4801 gcgagtatag ccgggtcttc atccggtgaa aaataatacg catcatccaa gcccaactca
     4861 tgcagaggcg gaatgtctgc acaaaagaca ggcaaatgac tgaatgcagc ttcaatcaaa
     4921 ggaataccaa acccttcttc acggctggga aagatcaatg catcggcgat acgaaaaaaa
     4981 tcagcgatca cttcatcggg taaaaaagaa tctgacaact ccgcaagaaa atacacagaa
     5041 tcctgcaaac ccaattgggt acgcaacatt ttgagttcat cgaagtattc acgattaagc
     5101 ggattatgtg gtcccagggg gccagtcacc acgagcgctg cctggggaaa actatttcgt
     5161 agttttgata gtgtttccag ggccagttca atgttttttc gacgagtgat tcgaaccggc
     5221 aaaagcagaa tcggtgcggc atccaaaagt tttgtctgct cgagcagcgt ttgagtctgt
     5281 agctgaagtt tgtaaaaccg cgcgacatcc acgccattgg gaacaacgta tacagacgga
     5341 ggatcaattt tcatgagttc cgtcaattcc ctgcgtcgta attctgagac aacaacatgt
     5401 cgactgttgc cccagtctgt tcgcaataag tcccatggat agccatcatg aagttccggg
     5461 agatagcgcg gcgtagtcca tgcaaggtcg tggtgccata ggatcagacc cggaagttgc
     5521 cgttcagcgt gtaacttgta aagcgcggcc gttaacgcaa ggttcttgtt taacgaacag
     5581 gcgttgtgga caatcaaaac atgtacatct tccagggcat ttcgcagttg cgtcgccagc
     5641 tcatcgcgca gcgaatcaaa atcctcggtt acatcgccac cagccaactg ttcctttaca
     5701 gccaacacgc gcggatcttg tgaatccacc agcggtagcg aaaccaaagg aatgtcatcg
     5761 cccagcgcgg atcctcgccc ggcaatcaaa cggacggcat gtccaccctt tgtcattaag
     5821 cgcgcgtgat gtgcaatgac actttccacg ccgccgacca ctggcggaac cgagtaatga
     5881 aggacagcga tgttcaaacc tgatatccca ggcaatctgc caggatggat tgcaaccgat
     5941 gctcaagaac tgtataagag aaataccgct tcgcaagttg ataattctca tctgcccatt
     6001 cttttgcgcg ctcagggttt tgaaggacct cacgaaccat gtcaagtgtg ttcctgctaa
     6061 tgaacccatc gaaccagacc gtacggaagc ccttgggctt gatatccact tcataaatgg
     6121 agtaattatt gaccaggatc aagcgatgat aatacacagc ttcaagaaac gcattgccaa
     6181 acccttcaat gctcgaagga tacgttacga gatctgcatg cgggtacaca tctccaaggg
     6241 tatagatctt gcgtccatct tccgtgagcc cgcgttcatc ctgaacctga tctgattcaa
     6301 agcggacagt cacatccatc aattgtgcat attcgcgtac acgttgttca taatcagaac
     6361 cttcatctcc tgcagcatgg gagatcacca gtttggcggg gagcccaaga cgatgcacaa
     6421 gttcaatcgc gtgctcgatc cctttgcgct ggatcacacg cgtgggctgc aggaaaaaat
     6481 attccccggg cccaatgcca aagtccgcac gcacagattc agtataggga tccaaatcag
     6541 gtggaggcga atcgaaatcc attacattcg ggataattcg agaggtcacg ccatggcgcg
     6601 atgcgatctg ctgcgcctga attgagttga tgaccacatg tcgaatggac ggcaggaccg
     6661 gaggaaacgc cgcagccaga tagtcaccca cacagttgac atgaaaacgc tgtcgctccc
     6721 aatggaagtc atggtggtga gctatggtcg ggaagcccgt ttcggcgaca aactcagtaa
     6781 tggcaagacc taacggcaga ttgagaggaa ttgttagcgc attctccaca ataaggattt
     6841 cgagttcaaa acggtgggca aattcatata attttacttt gaaatattcc ttaagctcat
     6901 tgacgcgccg ggtcatggca ggcgggcgga tatagatgga aaaaaaatcc ttatacaact
     6961 tcgcgatctc aggatgagcc tcgcgtgctg ctttggtcac aagccaactg cctgagtagg
     7021 cttgtacgtt gatcgcatca atctctgggt gccggtaaaa ggcctcgggc acgacgagac
     7081 ttcgctctgc cgggcgatca cattcgccgg caaagtaaaa acaatcgtgc ccaagctgtt
     7141 gcaataccgt tgcccatttt tctgtttcca gcgagacacc gtcagtccct gcgaaacgag
     7201 tggaaataaa accaatgtga tgtgagcgag gtttcatagc ttcatccatg aaatttgaaa
     7261 ggggttaaga gtaatctgtg cactctttac gggttgattg gtgagcagat ctatccccga
     7321 ttcgtacgtt gttgagaatg cgacttcctt gttgctgaca ttatgcaaac ataaggcgcg
     7381 tgacgttcca tctggtgaaa tacgttccag tgcaaagacg gaaggatgta catccaatat
     7441 tttttgcctg ccatgcgggt gaaatgccgc agaactactc cgcgccctga gcagctcgcg
     7501 atagtgagca aagacttgcc cgcgcagtga ctgtgggtct tcgagttcac gctgcaagga
     7561 ttgaagatca cacttctcac gattgaccgt gcggttgcga cccgtttgtc ccacaccatc
     7621 cagccagccc cgcgaaccaa acaggctgtg gaaataaatc ccgggcacac cgacaatgga
     7681 tagcataata gcttgtgaag tcataaatcg gtcgatttgc agcctcaaag gttcagtacc
     7741 caacggatca gaaagcgcat cgaagtaatt gatgttcatc tcatacgggc tttgcgtgcc
     7801 atctgaatta tgtttgtagg aaaccagtcc actatgggta agtgttttgt gcacaagcga
     7861 gtcgatatca gcgttggata aaatgcctcg agcgggattc agcccaattc catcgtggga
     7921 ggcgaggaag ttaaagaaag tcgttttatc agaaggaagc gtcagcgtct ttgcccaatc
     7981 cgacaagact cgtgcattgc ctgtatgaaa ggcatgcaga gtcaacggtg gcaaagcaaa
     8041 gttgtagacc aattgagctt cattcgtgcc atcgccaaag taggaaatat tatccacgtg
     8101 tggcacattc gtctcggtga tcaggtgaac gtgtggggca acttcgttta acgcggcgcg
     8161 caggaattgg atcacgccat gagtttgagg gagatggata cacgatgttc caatttcctt
     8221 ccacagataa ccaattgcat ccaggcgaat aaacgtcgcg ccgcgctcgg catacatgag
     8281 taggatatca aggatctcca tcatcacttc gggattttga acgttcaggt caatctggtc
     8341 atcgctgaag gttgtccaaa ctcgtttttc accagaaggt gtcgtaaatg atgtcagcaa
     8401 tggcagggcg cgcggacgaa ctacttgtga aagatccggc gaaccattca caacgacaaa
     8461 gtagtcacga taatgtggat catccttcaa gaaggcttga aaccagtcac tttgcgagga
     8521 gatgtggttg atcaccgcgt cgaacatcaa gcggaaatca ctctgcatgg cagagacatg
     8581 ctcccaatcg cctagcgccg gatctatttt gcgataatcc accacagaga agccgtcatc
     8641 agaggtccac ggaaagaacg gcaggaaatg aacgccgcca acgacatctt taagatatcg
     8701 cttgcaaaac gcgctcaagg tttgcagggg tttttcgctc gaggtctgca cctggtcacc
     8761 gtaagtgatc agaatggagt ctttttctgt gagcgcgcca tttcggacgg ggatgcgatc
     8821 aacatacgcc gcgaggatgt tttgaacgcc cgtcagcacg tgcggtgcgg catccgctcc
     8881 ataaagaaaa gtgagttgag ataagagatc tgtcattgtt gattcgattc tgattactcc
     8941 gtggtttctt acaacggaat aatttttgtg attcgtacat tctcccctat gggatgccag
     9001 aggcattcct tatatttttt taccacaatt cgtatgaatt aagattaatc cagaatgtta
     9061 acatgaacga aaattatcgc cgaaccgtac aatgcaacga attcccttca aaaatgtgat
     9121 ctcacgcaca agattaagtg aattttcagt ttggcgaggc ggggagccac atgacttcta
     9181 gcggcccgtg aaatactgca aagcgactct cagtcgctct tccacatctg taggtgcatc
     9241 tctcggcaga gattgaatcg cggattgtgc ttctacaacc gagtaaccca gcgcagtgag
     9301 cgcagccagg acttcactgt cataatctgc catggcagcg acgcctgcca gggcatcggt
     9361 gggcttgagt ttgtctttaa gatgaagcgc cattttttgc gcggtctttt ttccaacccc
     9421 tggaacacga ttcaagactt cgctctcatc tgcaaagacc gcccgctgaa tggcatccac
     9481 agtaagggtg gataatacag ccagcgcaac cttgggacca acaccatcca caccgagcag
     9541 ggtgttatac agttcgcgtt ctgcttgaga ctcaaagcca tacaaggtaa gcgagtcttc
     9601 gcggacgacg agatgcgtga acaaaagaac agattcaccc gccttggcgc gggtccggac
     9661 cggcgcgggg acaaaaacgc gcagccccac gcctcctact tcaaggatga tggccgtttc
     9721 ttcaatttga gagatttctc cgcgcagggt tgcgatcata aatgcatttt taccacagta
     9781 aataatgctg gtcgagtagc cctgagcgct tgcggcgaag ggcatatcga gaccagtcca
     9841 taatcaacgg tatcctgtac tgtgttggtc tcgatacgga agatcgccta ctcgaccaac
     9901 ggacctcaat catatctttt ggtattcaag tgggtaatgg cgatagcaag cgcatctgct
     9961 gcatcatctg gtttcgggat agagtccagt tgtagcaagg cgcgcaccat ctcctgaacc
    10021 tgtcttttat ccgcgctgcc atatccggca accgcgtttt tcacttcctt gggggtgtat
    10081 tcgaaaacgg caacgccggc ttgttcaatg gcaagcatca ccacaccgcg cgcctgcccg
    10141 acagcgattc cggtcgttac atttctcgcg aagaacaatt tttcaaccgc agccaattga
    10201 ggtttatatt catggagcaa ctcttgcagt tcattgtaga gcatcaccaa ccgcgcagag
    10261 gcagttgcat cctttggggt gaggattaca ccatacttaa cggcgaccag agtcccatcc
    10321 ggctccatgc ggacaagtcc atatccagtt gtagcagtgc cggggtcaat ccctaatgcg
    10381 agggtcattt taaataattg taggggcacg acgtgtcgtg cccctacgaa atttcaaatt
    10441 aagcagcttc gagcgccgct atggcttcat ctgagacttt aagattcgag tatacgtttt
    10501 gcacatcgtc cagatcttca atggactcaa gtgccttgag cacctgcact gaatcttcgg
    10561 ggcccagttc catttcctgt ttggggctca ttcgcaagcc agcttcttca ggctggacgt
    10621 tcgctttatg gagcgcatcc gcgatgatct tgaacgactc aaccggtgca ataatttcaa
    10681 tatgaccttc ttcctcaatg acatcatttg cgcctgcttc gatgccgagt tcgaaggctt
    10741 tgtcgaaatt catcgcactg gaaggaaacg agaagtagga gatgcggtca aactgccagc
    10801 cgaccgcccc aagctccgcc atgttgccgc ctgccttgct gaatgcatga cgtaaatcag
    10861 agatcgtacg gttacgatta tcggtaacgg tttcgaccat cactgccaca ccgtgtgggg
    10921 catagccttc gtaggtgatc tgttcaaatg ccgcgctgtc tttgtcttcg ccggtaccac
    10981 gcctgatggc gcgttcgata ttatccttgg gcatgttttc agcacgggct ttgtccactg
    11041 ccaggcgtaa acggaaattg gaatcggggt cacctccacc gtctcgggcg gaaagcacaa
    11101 tttcgcgcgc aagacgcgta aaaatcgcgc cgcgcttggc atctgccgcg ccttttttgc
    11161 gtttgatggt tgaccacttt gaatgacctg acatggggag ctccttacac tatgaacact
    11221 attgtgtgaa tttacacttg tatacgtgaa ttataccatt tggaaaagaa gggcgagacc
    11281 ttggtctcgc ccttctggtt acatcgcctt cgcccgctct gccatttgat ttcgcaagtt
    11341 attcgtatct ttatcgattg tgaacagtgc accgagatag aagcaaaaac acaatatcca
    11401 tgcagtgaca cagatcaaga gaatggaggt cttgaggttg gttgcatctg caatgatacc
    11461 cgctaaaaac ggcgcaaagg ccgctccggt gttctcgatg aaatattcaa cagcctgcgc
    11521 cgtcgaacgc acctcaggga ctgtcacatc atagacagta gcaatcacgt ttgctgaaga
    11581 aagcgggata aaaatggccg tgataaccat cagaataaaa aactgattgc gtgcctcgac
    11641 cggcgtgttg atcgccagaa acaaaaagat cgcgcccagc aatacaccca cgcatgagat
    11701 caggatgcgt cccttgttgg tgcgcttgaa gaggtaatct cccagtgcgc cgcccacaaa
    11761 gtagccagcc gccagcacca ggatcacggg tgccattgta aagagaatac tgttctggtc
    11821 atagccacgc tcctcttcca gatacgtgaa gaaccaatag acgatcacat tccatgggaa
    11881 gactccagca aacccttgaa gaaaaacgaa ccacatcgtt ctctttttga agatctgcct
    11941 ggcttcctcc caggagaaat gaaagcttgt catttccgcc atattctcga actcaggctc
    12001 ggctttgccg cgcggcattt ccttgatgcc gaagaaaatc aggatggcaa gcacaattcc
    12061 cagcgaccct gttgcgatat agatggtccg ccaatcccag cccagccctg gagcgatcat
    12121 cagtgccagt acggtgccaa acagatagcc aatcggctgg ctcaattgca gcaaaccata
    12181 gactttgccg cgcaaatttg gaccaaaata gtcggcgacc agggtgaaaa tacccggata
    12241 agatgaatca tcaattcctg tggaggcgcg tgttgcaaca aactgcggga acgtcctcac
    12301 gacagaactc aaccaggtag tcgcccccca aatgaaggag gcaagcgcaa gtaattttga
    12361 acgggcatat ctatcatata aataccccca tagcggataa aaaattgtcc ccacgatgag
    12421 cgagcctgtc aacacggcgc ccatctgtgt tttggtaatt tgaaacgaat ctcgaatctg
    12481 actttccagt ggaccgatca gcaacttgtc tgtttgatgc aaaagcatga aaaagaaaaa
    12541 gacagcgacg acgaaccagc gatagcgtga atgatcggct gacatagtta cctccactat
    12601 aaagaagtat gaatagttta taacagaaca agcaggcggt caatcaaacg aacaacctcc
    12661 cgggcgggag gttgttcagt tgaaacgtca ttggaattat cggttgatga tgaatagaat
    12721 gccggcaatc aacgcacaga ttccagcaat gaggttgagc acgtttcctc ccaggctcac
    12781 accgagcagg gaaagcccaa cgatgaccaa atatattcca agaaggatcc attttagtcc
    12841 acgcatctta ttctcctttt gttttgaaca gcaattacaa agatgtcaaa ctctgtgaat
    12901 aagaacgttt tatgacccca gaagttacgg atttgccgta ccgaacgttt cgtcagaaat
    12961 gatttcctga gccagttgct gcagatattc tgaatcgccc gtctttgttg cgcgccagta
    13021 ttggtccagc aattcaagtg gactcaaact gccgaccgtt tgaccttctg ggaggcggac
    13081 gcgggcttcc acttttggct tctttgccag atgaaactca aaggctccca ccgtatactc
    13141 ccgtagcgcg gactcatcga tcagcacatc catttctctc ggatattcaa cagtcagccg
    13201 gacgatggca ttcgccatat ttctgggatc tggaagagcg gcttttagaa cgtccgtcac
    13261 attctcattg gattccaaaa cgatccattt atcaacgaac tgacgacctg tcagttttcg
    13321 ccattcgacg gttgagtagc cgcgtgttat ctcggcaatg acaaagaact tgtcgtcgtg
    13381 cgcttcgccg aagtcgatac gttcgatcga accgggataa atcaccggcg gatgttcgcc
    13441 gcgattgaca tcctgccact tgtggatatg ccccatcgcc acataatcca gccgtttatc
    13501 tttcactagc gaaacaggca ggaccaggtc acttcccagc atgacggtgc gctctgcacc
    13561 gaacaccgcg ccttccaccg aagcatgggc tgtaagaatt aatggcaggc cgggatccgc
    13621 ttccttgatc cagtcttcga ccagttctga gattcgattt tcgatctttg agaaaatctc
    13681 gctggtctcc gtggcgcttg aagccatcgc cgccatcagt cctgatcgcg acacccaggg
    13741 catggcgatc acctgcacag gtaagcccca caaatctttt gataataaga attccggctt
    13801 ttggaggacc cgtatatatg gaacttgaag cgtatcaaac tcctgaatcg catgggcgcg
    13861 tccgatcgct ggagagagat cgtgattacc caccaaaagc aaggttggaa ttttggcttg
    13921 tgacaaacga acaatgcgct tctcccactc acgttggaac gtaggtgcag gtgagcggtc
    13981 tttgtatgca tctcccgcga agatcaccat atccacttgt tcagagatgg ccgcgtcgat
    14041 gatcgtatcg agcgatttca gaaaatccag tacgcgtaag ggcaacccgg tttccgggtc
    14101 atggcgtccg taattggcca tatcaatgtg ggcatcagca aagtggagta tcttcatagt
    14161 ttaataaaac tccggtggtt cagtagacag cgatgttcgt ccgctggcat atcgaaacca
    14221 ccaataacct tcaagcaaga tatttgatgt taccaggtgg tttcgacacg tcccgcgaaa
    14281 agcatgctga actactgaac caccgaacta aggttgaaat cctaaatcct gcaaagcttg
    14341 aatggccggg ggccaatttg gatgcagtcg aagggcctca cggaaatcat caacggcgtc
    14401 ctgtgtatta ccgatcatat atttcgcgcg accgcgccaa tagatacttt cctcgagagt
    14461 aggcttggag atcgtctctg ttagtgtgat attagccagg ttaactacat cctgataacg
    14521 agccgaataa tagtacgccc agtagggacc tgtttgatac cacatcatcc ggtatgggcg
    14581 ctgtttatca tcttcgccaa tggaatcgta aatggaaaaa gcctgatcat acgcagcggc
    14641 tgcatccgca tattcctgta aagctacatg gctggagcct ttggcaaacc aggcaaaata
    14701 caaattaatt ccgttcgtac cccggatatt ttcctctgcg atactgagcg cctgcctcgc
    14761 agcccagttg gcatccgcat agggaccaag cgttgccaat agtttagcct cctgctcagg
    14821 cgggtacacc acgatgaaaa gattactgaa agaaagccat gcctcactcg ccatgaattc
    14881 atcataagga atgcgaaagt tgggaccatc gttccaggta tcctgaacgg tcagttcctg
    14941 cctggcgtca tcatacccgg tgacaaattg ataatgcccg agccaggcaa cccggccggt
    15001 gtaatctttt tcataatagc ctttttcggc aatgaatgga aaacccgccg ccaacagact
    15061 ctttgccaat tcaagatcgc ctcccagccg ggataaggcg cgaaactcgg tttcgtcgtt
    15121 aacgaagtcc accatctcga acggcatgac gttaacatct gaccggcctt gttggatgaa
    15181 gtccagtttc ggatccggca caccgggctt gatggctctg gcgacatcgt cgcggtcacc
    15241 atcccagccg acgaagttca gtgccatact taaattggcg ggaccgcaat agttccagcg
    15301 attcttttga tcttcgtacc ggaagcctgc taacttaacg gaagccggca aaggcgtcgg
    15361 tgatagtgtt ggcgtcagtg tggggccacg tgtgggagtt gtcgtaacag taggcacttc
    15421 cggggtcaaa gatgcaatag cagtctgcat catctccgta acggcaaggt cgatttgcgc
    15481 ttgttgcgca ggttgaaaca cagcctggtc aggcggattg atcctgtatt tgatatctgc
    15541 catccatcca tcgatgcgcg gcatagcacg actgtacaca ggtggcagga agaagagacc
    15601 aatccccgct aaaataacaa tgggaatgac ccatttgcgg ttaaaacgat tttgcatgag
    15661 gaggattata aaacaaatcg acctctatac tttacagccc ctacccgtct ctaatgtatc
    15721 tattattctt tgatttcgcg cgattcatcg tcagaattgt ttttttatgg ctgaattccc
    15781 aaatcctgta atgcctgtgt agcgggactc cactttgggt gaactttcag cgcggcacga
    15841 tagtcccgga cagcaaggtc ggtttcgccc gccatgtaat acgccattcc gcgccagtac
    15901 agggattctt ctagcacggg ctcggcaatg gtgtcgttca aggtagtgtc cgccaggctg
    15961 atgacatctg agaatcgatc agtgtaataa taggctttgt acggaccggt ctggaaccac
    16021 atcatgcggt atggacgaat ggaactatcc acctgaagat tggcgtagat attgaatgca
    16081 cgatcgtaag cgatggcagc atcggtatat tcgaacaggg cgacatgact cgtgccggca
    16141 ttgaaccagg caaagtattc atcaacatcg gtcagagttt gtgattcggc acgcgcaact
    16201 tccaaagcgt gacgattggc ctgttcctcg tctgccaggg ggccgagcag agtcagcacc
    16261 tcattctgtt tttctatagg atagaccaca acaaatacat aattgaaggc gcgccagcgt
    16321 tgggtcagct tttcataact gatctcccgg ttggggcccg gattcgttgt gggttcaggc
    16381 tggtagctat cctgatagat gatcatctga ttagcatcgt catacccggt cacaaaaccg
    16441 taatgcccta gccagctatt tttgccattg atatcgactt cgtacacccc gatttcgaca
    16501 atcaccggga accctgcaga gacgagccgc ttgagcgttg cgatatcccc accgttacga
    16561 atgatggaag tcatacccgg tacgttctcg gtgatgtaat cctgaagttc ataaggcatt
    16621 acatttttat ctttttcgtt gattggcatg accaccttgc cgatcacatc gcgatccccc
    16681 tgccagcccc aataagtgag agccatcgag aaattggcag gtccgcaata attacgtcga
    16741 ccatgctgct gctcatattt gacattttcc aacagaacgc tggcaggcag aggcgtcgta
    16801 gtaatcgtgg gtcggactgt agggctaggg attggcgttt gagtctttgg agattgcatc
    16861 gtctgagtcg cggcaatctc agctggcgat tgttgttgag gttggaaaac ggcttcctca
    16921 ggcgggttga gaaaatattt gatctgtgta cgcacaaact ccagccgcca tgcaaggcgg
    16981 ctgtgaacag gcgggatata gtagattccg atgatcagca gaatgaaaac agggattgcc
    17041 cagatacggc tataacggtt ttgcacgacg aggattatat gacaaaaaag acccttaaga
    17101 ctttgaaagt cctaagggtc tcactttgac ttttaactat tcttttactt cgccttcgat
    17161 cacatcgtca tcgcctgatc cagagggagg cgtggatgga ccaccggcgg cttcaggctc
    17221 aggctgtccc tgtgcgtaga gctgctggct caaggcatgg aaagcctgtt gtacggcttc
    17281 tgaagcttgc tgaatgcggc tcaggtcatc gccctgtgca gcggttttga gatcgttgat
    17341 cttcgtctcg atttcaccgc gctcggcaga ggggacctta tcgcccagat ctcgcagggc
    17401 cttctcagtc tgatagacga gtccatctgc agtgttcttc gcatcgatca gctcacggcg
    17461 cttcttatca tcggcttcat gagaacgagc ttcctgaacc atgcggtcga tatcgctctt
    17521 gtccaaattg gtcgaagcgg tgatcgtgac cttctgctcc ttgcccgatg ccttatcctt
    17581 cgccgtcaca ttcaggatgc cattggcatc aatatcgaac gtgacttcca cttgcgggat
    17641 gccgcgcggg gccggtggga ttccatccag tcggaatcgt ccgagagaca tattgtcagc
    17701 cgccatcgag cgctcaccct gcaggacatg aatgtccacc gcggtctgat tatcagcagc
    17761 agtcgagtag gtctccgtct tgcggacggg gatcgtcgtg ttgcgctcaa tcatcttggt
    17821 catcacgctg ccaagagttt caacgccgag ggaaagcgga gtgacgtcga gcagaaggat
    17881 gtctttgaca tcaccgccga gcacaccacc ctgaatggcc gcgccgatcg cgacaacttc
    17941 atcaggattg acgcccttgt tgggttcctt gccattggtc aacttgcgga ccaggtcctg
    18001 aaccatcggc atgcgtgtcg aaccaccgac gagcaccact tcattcagct tgccggcatt
    18061 gatgccagca tccttgagag cggcttcgaa cggcttgcgg cagcgctcga gcaggtcttc
    18121 cgtcatctgc tcaaatttag cgcggcttaa cttcaactgt aaatgtttcg gaccggttgc
    18181 atcggccgtg atataaggca gattgatttc cgtctccatc actgtggaga gttcgatctt
    18241 ggctttctct gccgcctcgc gcaaacgctg gagcgcctgg cgatcactgc gcaggtcaat
    18301 gccctgatct ttcttgaact catctgcagc ccagttgacg atgcgttgat cccagtcgtc
    18361 accaccgagg tgtgtatcac cgtttgtggc tttgacttcg atcacgcctt cgcccacttc
    18421 aaggatggac acatcgaagg taccaccacc aaggtcaaag accaaaatgg tttcgttctt
    18481 tttcttatcg agaccataag ctaacgcaga ggcggttggc tcgttgataa tgcgcagcac
    18541 ctcgagacca gcaattttgc ccgcgtcttt tgtagcctga cgctggctgt cgttgaagta
    18601 tgcaggaact gtgatcacag cttgcgtcac agcctgacca agataggcct ctgcatcagc
    18661 cttcaactta cccaggacca tcgcagaaat ttcctgtgga gaatattcgc gattggtaac
    18721 gggaatcttt acgcgcacat cgtccgatgg gccctgcaca acatcatagg aaaccatctt
    18781 gcgctcggcc tcagtttcgc caaagtggcg gccgatgaat cgcttgatgg agtaaattgt
    18841 attttcaggg tttaccactg cctggcgctt cgcagtctga ccaaccagcc gttcaccgtt
    18901 cttattgaac gctacaactg acggtgcaag tcgtccacct tctgcagtgg agatcacggt
    18961 cggcgaaccg ccttccatga ctgcaaccac agagttcgtc gttccaaggt cgataccaat
    19021 aattttgctc atagagatac tcctattcgt tcttgcgctt atttcaattt gcttgcgcaa
    19081 tgacggatat tattttcaat gtttccatct tacctgctat gtgcaagaat actgttaaca
    19141 aaatgtagaa tttagattgg atttacccaa ccccctccgc ccttcgggca cctcccccaa
    19201 actcgcattt ggaactgcat acattatcca aacttatgtt gcgtatttgg gggagattgg
    19261 gtgggagtta aggttcacgc actgtga
//
LOCUS       DASTJP010000503         9276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1497232, whole genome shotgun sequence.
ACCESSION   DASTJP010000503 DASTJP010000000
VERSION     DASTJP010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497232"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3122)
                     /locus_tag="VFQ23_14735"
     CDS             complement(<1..3122)
                     /locus_tag="VFQ23_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907905.1"
                     /translation="MVNDTTSTARLLRVIIIALLIFPVMMPKRARALTRAESAGESAV
                     VQEGAFGISRQGRQDSGLLAPLAGILPPSTPFLPSVLVNTIDTSVWDHPSSDPAGIAY
                     QASTGKLLISDIDVEETPQTFWEGFNIFQSTLTGSLSGNCTTFTSTPLNLSSYNNFSD
                     EPAGITINNSNNHIFFSDDNASRIFEVAPGTDGVYCTSDDVVTYVAVGVPPYSIPDTE
                     DLAYGQNKIYITSAGDGNKTIYTYDLGANGVLGGGDDSSGPLPSFSTDALGFGDTEGI
                     AFNSVAGTLYILSTTGPDTYLGEVTTSGTLLTAYDLTYLGSANRSGLTVAPASGDPST
                     NNIYIVSRGKDNGSATDFNDGKIWEIDITNPVMPDMIFKDGFESGNFSAWTSSTINTG
                     NLNVDPSAALLGSYGMRAQIINTIGMEVTDDRPTLEKHYRARFYFDPNSIMLNGDIHN
                     IFQGQKYDPYTSSPGAYNTTVFAIQFQSSGGVYQIRGRAFSDTAAVLSTGWQTMTDAP
                     HFIEVEWSAATAAGANNGNFWVWLDNVHVLSINNIDNDTRVIDRARLGPTSVIAFASG
                     TYYFDAFESRRQSYIGPVVSPTVLSVLRAGSNPTSASSVNYAVTFSESVTGVDSSDFQ
                     ITPTVVTGATVSGVSGSSTTYTVTVNTGTGTGSLRLDVVDNDTIKDSEGNFLVGGFST
                     GESYTIVEDAAVAVNIAQVNAGNFNIPVHTGDRYGFPGVDNGPAEVRSTNGVDILPAL
                     RVIWREPGIRTSYSEMMGLPVEQLSSEYWFPWYNNMDVASMDQGFRIANVDTVNSNTV
                     EVWVGTSKLDTVTLTPGESIRKGYAANNGPARVVCTSCNPLGASDRILAALRVIWKEP
                     GSRYSYSEMMGLPVEQLSTEYWYPWYNNLDVASMDQGFRIAIVNGSGDNTVQVLVGGV
                     QVGTNITLVPGASVRVGYPVNDGPVQVRCTTCSGAEKIITSLRVIWKEPGFRATYSEM
                     MGMPLEQLSTTYWFPWYNNADVNSMDQGFRIAIVNGSGDNTVQVFVAGALQESITLTE
                     NESVRVGYNA"
     gene            complement(3471..4718)
                     /locus_tag="VFQ23_14740"
     CDS             complement(3471..4718)
                     /locus_tag="VFQ23_14740"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9907906.1"
                     /translation="MDSQKDVVIIGGGVIGVCSAYYLAQKGIGVTLLEKGEIASGCSY
                     GNGGLIVPSHAVPLASPGALGNGLRWLFDSESPFYIKPRLDLDLINWLVRFAFASRHN
                     QMLQAVPVMRDLLFASRALYEELAKTAGFDFGFEGTGSLLVYLSKEKLQHEMEEAHLL
                     ERFNIPIEAMDQTQVQEREPALLPDVIGGIYYPRDGHINPHRFVTGLAEKAQRLGAQI
                     CVQTEVLGFESSQGKIKRIKTTDGMIAPKQVVLATGSWSPDVARDLKLRIPIQAAKGY
                     SITLENPQVSPKIPLSFGEARVVVNPLGDSLRLAGTLEMAGMDSSINSRRIDTMRKAS
                     NGYLRGLEYAKVIETWRGLRPCTPDGLPIISRSQRFENLIVAAGHAMLGMSLGPVTGK
                     MVSQLIHEEKTDVDIFPFRMERF"
     gene            complement(4721..5449)
                     /locus_tag="VFQ23_14745"
     CDS             complement(4721..5449)
                     /locus_tag="VFQ23_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907907.1"
                     /translation="MTAIECEKDIVKVSDLHFAYPDGHAALRGVSFHLCAGDKVALVG
                     PNGAGKSTLMLQLNGILNGQGEIEISGMRLTRDNLPVIRALVGLVFQNPDDQLFSPTV
                     FEDVAFGPLHMGLPEEEVLARVDSALEAVRMASYRDRLSHHLSVGEKKRIAIATVLSM
                     NPSILILDEPSAGLDPRARRTLINLLRDLPITMLISTHDMRMVQELFPRTIVMDGGQI
                     VADGMTNEILDDEKFLNEHGLEKP"
     gene            complement(5446..6240)
                     /gene="cbiQ"
                     /locus_tag="VFQ23_14750"
     CDS             complement(5446..6240)
                     /gene="cbiQ"
                     /locus_tag="VFQ23_14750"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HET9907908.1"
                     /translation="MHSDVFDRYHHGHSLIHDLDPRIKVVVAIAFIISNALLPDGAWT
                     AFALAWSLVLIANVLSGLGISFTFRRSFIALPFALAAITVLFSIPGEPLVTVRLLMMN
                     LTITDAGFLRFVSIVIRSWLSVQMAILLVATTEFPKIVHALNHLRVPTILTVIISFLY
                     RYLFVLTDEVLRMLRARQARSAAVSGINAGGNMLWRARIAGHMAGQLFLRSYERSDRI
                     YNAMLSRGYKGELMTIHPHHFHNSDLWIGAFAILVLILIQVAGRLS"
     gene            complement(6321..7271)
                     /locus_tag="VFQ23_14755"
     CDS             complement(6321..7271)
                     /locus_tag="VFQ23_14755"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HET9907909.1"
                     /translation="MHFFIAPHSPEPLHIPDGFLNLVVSLICWAITALTLSMAISRTN
                     KSLGEKQVPLMGIMAAFIFAAQMINFPVAGGTSGHLLGGALAAIVLGPWAGMLVMTAV
                     IAVQALILQDGGLLVMGANILNMGLLTAAIGYGLYRSVSGSSRGAKLAVAGVAAWLSV
                     MAGALATALQLWLSGTSNLQTVVIAMLGVHALIALGEALITVAALGFILQTRPDLLDE
                     KSVSAQGGRGWVVAGIFISLIVVLLSPFASANPDGLERVAQDLGFISIAQDAPFQILP
                     DYTVPFLGETSLSTIVAGLIGALVVLGIAIFAARSLQKKT"
     gene            complement(7291..7707)
                     /locus_tag="VFQ23_14760"
     CDS             complement(7291..7707)
                     /locus_tag="VFQ23_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET9907910.1"
                     /translation="MSCAVDYVPQLRARGFRMTPQRLAILHVLHHEGTHLSPTEIYKR
                     AKKDFPRLTEPTVYRTLEFLTQNGLVRPSHSHGRHLTYQIAGDDHHHVVCRICGSEIE
                     VDHALVENLYRKLESTSGYLRIDSHVTFFGICPQCQ"
     gene            complement(7786..8514)
                     /locus_tag="VFQ23_14765"
     CDS             complement(7786..8514)
                     /locus_tag="VFQ23_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907911.1"
                     /translation="MKSRLSTIALLASMLLLTACGSPAPVATSTLAQLATAVTLPPTN
                     GTLIPTATPPLSLPTSISSTVIPTLTLSVPTSAASDLCADPQVLALIDSLKTAALNSD
                     GPLLSSLVSPQRGLDVARFRDGTVINYRPDQAKFLFETTFEANWGPAPGSGLEEIGSF
                     HDVVVPELVKAFNQPYTLHCNELKHGGATYEVSWPYEGGFYSVHFPGTEVNGFMDWHT
                     WAVGIEHVNNKPYLYALIPFFWEP"
     misc_binding    8666..8982
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2473 a         2397 c         2259 g         2147 t
ORIGIN      
        1 ttggcattgt aacccacacg cacactctca ttctccgtca gcgtgatgct ttcctgcaag
       61 gctccagcga cgaagacctg gacggtgtta tcgccactgc cattgacgat ggcgatgcgg
      121 aagccttgat ccatggagtt gacatcggca ttgttgtacc aggggaacca gtaggtggtg
      181 gagagctgct cgagcggcat gcccatcatc tcggagtagg tagctcggaa accaggttcc
      241 ttccagatga cacgcagaga ggtgatgatc ttctcggctc cactgcaggt ggtgcagcgc
      301 acttgaaccg gaccatcatt gaccggatag cccacacgga cacttgctcc cggcaccagg
      361 gtaatgttcg tccccacctg aacgcccccc accaacacct ggacggtgtt gtctccactg
      421 ccattgacga tggcaatgcg gaagccttga tccatggagg cgacatccag gttgttgtac
      481 caggggtacc agtattcggt ggagagttgt tcgacgggca gacccatcat ctcggagtag
      541 ctgtagcgag aacccggttc tttccagatc acccgcagcg ctgccaggat ccggtcactg
      601 gcacccagcg gattgcaact cgtgcacacc acccgagctg gtccgttgtt ggcggcatag
      661 cctttgcgga tactttctcc gggagtgagg gtgacggtat ccagtttgga tgtgcccacc
      721 caaacttcta ccgtgttgct gttcacagta tccacattcg cgatgcggaa gccctggtcc
      781 atcgaagcga catccatgtt gttgtaccag gggaaccagt attcagacga cagttgttcc
      841 accggcagcc ccatcatctc cgagtagctc gtgcgaatcc ccggttcccg ccagatcacg
      901 cgcaacgctg gcaggatgtc caccccattt gtacttctga cctccgctgg cccgttgtcc
      961 acacccggaa aaccatatcg gtcgcccgtg tgaactggaa tattgaaatt gcctgcattt
     1021 acctgtgcga tattaactgc aaccgcagca tcttcaacga ttgtgtatga ctcaccagtg
     1081 ctaaacccac ctaccaggaa attgccttcg ctatctttga tagtgtcatt gtcgacgaca
     1141 tccagtcgaa gtgagccggt gcctgtccct gtattgaccg ttaccgtgta tgttgtactc
     1201 gagccactca ccccgcttac tgtcgcgccg gtcacaaccg tgggtgtaat ttggaagtcc
     1261 gatgaatcca caccagttac agattcagag aaggtcaccg cgtagttcac actggatgca
     1321 ctggttgggt ttgaacctgc acgcaagacg ctgaggacag ttggagagac gaccggccca
     1381 atataactct gccggcgaga ctcgaaagca tcgaaataat aggtgccact tgcaaaagca
     1441 attacagatg tcggaccaag tcgggcgcgg tcaatgaccc gtgtgtcgtt atcaatattg
     1501 tttatgctaa gaacatgaac attatccagc catacccaaa agtttccatt gtttgcaccc
     1561 gcagcagtcg cagccgacca ttccacctcg atgaagtgcg gagcatctgt catggtttgc
     1621 caacccgtgc tgagcacggc cgcagtgtcc gagaaggctc tgccgcgtat ttggtacact
     1681 ccaccgctgg actgaaattg gatggcaaaa actgttgtat tgtatgcgcc cggtgagctt
     1741 gtatacggat catacttttg gccctgaaaa atattgtgga tatctccatt cagcataatg
     1801 gaattggggt caaagtaaaa acgcgcccgg tagtgttttt caagagtagg acgatcatcg
     1861 gtcacttcca taccgatagt gttgatgatt tgagccctca tcccgtaact tcccagcaac
     1921 gcggccgaag gatccacatt caggtttccg gtattgatcg tgcttgacgt ccaggctgaa
     1981 aaattgccag attcgaatcc atccttgaag atcatatcag gcatgaccgg gtttgtaata
     2041 tcaatctccc agatcttgcc atcattgaaa tctgtggcac taccattgtc ttttccgcgg
     2101 ctgacaatgt agatattgtt tgtggatgga tctccactgg ccggcgcgac cgtgagacct
     2161 gaacggttcg cagaacccaa ataggtgaga tcataggccg ttagtagcgt gccactggtt
     2221 gtcacctccc ccaggtatgt atccgggccg gtagtactga ggatgtaaag cgtccctgca
     2281 acggaattaa aagcaattcc ttctgtatcg ccaaagccca aggcgtctgt gctaaaactg
     2341 ggcaatgggc ctgagctatc atctcctcca cctaacacac catttgcgcc cagatcatag
     2401 gtatagattg ttttgtttcc gtccccagcg ctggtgatat aaatcttatt ttgtccatag
     2461 gccaaatcct cggtgtcggg aatgctgtaa ggaggaaccc ccaccgcaac atacgttacc
     2521 acatcatccg aagtgcaata tactccatcg gtgcccggcg caacttcaaa aatcctgctt
     2581 gcattatcat cggaaaagaa aatatggtta ttactgttat tgatcgtgat tcccgccggt
     2641 tcatctgaaa aattattata agagctgagg ttgagaggcg ttgaggtaaa ggtggtgcaa
     2701 ttgcccgaga ggctgcctgt cagtgtggac tggaaaatat tgaaaccttc ccaaaatgtc
     2761 tgcggcgttt cttccacatc gatatcgctg atcaaaagct ttccggtcga agcctgatac
     2821 gcaatcccgg ctgggtccga acttggatga tcccaaacgg aggtatcgat tgtgtttacc
     2881 agcacgctgg ggaggaacgg tgtgcttgga ggaagaatac cagccagcgg cgccaataag
     2941 ccagaatctt gtctgccttg acgagagatc ccaaatgcgc cttcctggac aactgcgctc
     3001 tcacctgccg actcggcccg cgtcaacgcc cgggcacgct tcggcatcat aacagggaaa
     3061 atcagtaatg cgataatgat gacgcgcagc aagcgcgccg tactcgttgt atcgttgacc
     3121 atggcatacc tttactattt ttaggattat gacagcttgc aaccggatcg taactggctt
     3181 tccagcgtcc caatttgttg ttatacaaac ttcaattcag ttgtgattaa ttattcttct
     3241 taatcaaggc caaccttgcg tggctggtat ctcaacattc gcaccattac atggttacaa
     3301 gaccatcacc ctatagttag gttacaagca tcgtcacatg gattggttac gcaagagaac
     3361 aagaacaggc atcagtgaaa aactgatgcc tgtttcgttt aatacgacta tggataagat
     3421 tgattttggt gtaaggactc aaaagcttca gattgttgcg aaaaatgaac ttagaatctc
     3481 tccatccgga atggaaaaat atccacatcc gtcttttcct catgtattag ctgagaaacc
     3541 attttcccgg tgacggggcc taatgacatg cccagcatgg catgccctgc ggcgacgata
     3601 agattctcaa atctctgtga acggctgatg atcggtaaac catctggcgt gcaggggcgg
     3661 agtccgcgcc aggtttcgat aactttagca tactcgagac cgcgtaaata tccgttagat
     3721 gctttacgca tagtatcgat gcgacgtgag ttgatcgagg agtccatgcc agccatttca
     3781 agcgttcctg cgaggcgtag cgaatcaccc agcggattca cgactactcg agcttcgcca
     3841 aaggagagag ggatttttgg tgacacttgt ggattttcaa gtgtgatgct atatcccttt
     3901 gccgcttgaa taggtatgcg aagtttaagg tcacgcgcca catccggaga ccaggatccg
     3961 gtggcaagaa ccacctgctt tggggcaatc attccgtctg tggttttgat tcgttttatt
     4021 tttccctgcg aggattcaaa acccagcacc tcggtctgga cacaaatctg cgcgccgaga
     4081 cgttgagcct tttctgcaag ccccgttaca aatcggtgcg gattgatatg tccatcgcgc
     4141 ggataataaa ttcccccgat gacatctgga aggagagccg gttccctttc ctggacttgt
     4201 gtctgatcca ttgcctcaat cggaatatta aacctttcca acaagtgcgc ctcttccatt
     4261 tcatgttgaa gcttctcttt ggaaagataa accagtaaag aacctgtccc ctcaaaccca
     4321 aaatcaaacc ctgctgtctt cgcaagttcc tcatatagag cgcggcttgc aaatagtagg
     4381 tcacgcatga ctggaactgc ttgaagcatc tgattatgac gactggcaaa tgcaaaacgc
     4441 acaagccaat ttattaagtc caaatcaaga cgcggcttga tgtaaaaagg gctttccgag
     4501 tcgaagagcc agcgcaagcc atttcccaac gcacccggtg aagctaaagg gacggcatgg
     4561 ctcggcacga tgagccctcc attcccatag gaacagccag atgcgatttc tcccttttca
     4621 aggagagtaa ctcctattcc tttttgcgca agataatatg ccgaacaaac gccgatcacc
     4681 ccaccgccga tgataacgac atctttttgc gaatccatga ctatggcttc tctaacccat
     4741 gttcattcag gaacttttca tcatccagaa tttcgtttgt catcccatcc gccacaatct
     4801 gaccaccatc catcacgatc gtgcgtggga agagttcctg caccattcgc atgtcatgcg
     4861 tcgaaatcaa cattgtgatc ggaaggtcgc gcagtaaatt aattaacgtc cgtcgggccc
     4921 gcgggtcgag ccctgcggag ggttcatcca aaatcaaaat actcggattc atggataaaa
     4981 cggtggcgat ggcgatccgc ttcttttcac caacgctcaa gtgatgtgaa aggcggtcac
     5041 gataggaagc catgcgcacc gcttcgagag ccgagtcaac tcgagcaagc acttcctctt
     5101 ctggcaaacc catgtgaagg gggccaaaag cgacatcttc gaacacggtc ggtgagaaga
     5161 gttggtcatc cgggttttgg aatacgagtc caaccagtgc acggatgaca ggcagattgt
     5221 cgcgcgtgag gcgcataccg ctgatttcaa tctcgccctg cccattcaga attccgttca
     5281 gttgcagcat caacgttgat ttgcctgcgc cgttcggtcc gaccaatgct accttgtccc
     5341 cggcgcataa atggaacgaa acgccccgca gtgccgcgtg cccatcaggg taagcaaaat
     5401 gtaagtcact gacttttaca atatcttttt cacattcgat agctgtcatg agagccgtcc
     5461 tgcaacttgt atgaggatga gtaccagtat ggcgaaagca ccaatccaca gatcactatt
     5521 atgaaagtga tgcggatgaa tcgtcatcag ttcaccttta tacccccgag atagcatcgc
     5581 gttatagatg cggtcacttc gctcatagct tcgcaggaac aactgacctg ccatgtgacc
     5641 ggcaattcgc gcccgccaga gcatgttccc gcccgcattt attccggata cggctgccga
     5701 acgcgcctgc ctcgccctga gcattcgcag cacctcatcc gtgagcacaa aaaggtaacg
     5761 atacagaaaa gaaataatga ctgtaagtat ggtgggaaca cgtaaatgat tcaaggcgtg
     5821 gacaatcttg ggaaactcag ttgtcgccac cagcagaatt gccatttgca cagacagcca
     5881 ggaacgaatg acaatactta caaaacgaag aaagcctgcg tccgtaatgg tgagattcat
     5941 catcaacaaa cggacagtga caagaggctc accaggaatg gaaaaaagaa cagtgatagc
     6001 cgcaagagca aaaggcaagg caatgaagga gcgcctgaag gtaaaactga tgcccagtcc
     6061 tgaaagcaca ttggcaatta acactaatga ccatgcaagg gcaaaggctg tccatgcgcc
     6121 atctggcaga agggcatttg aaatgataaa cgcaatcgcc accacgacct tgatacgagg
     6181 atcaagatcg tggatcaggc tgtgcccatg atgataacga tcaaacacgt cggaatgcat
     6241 ttttactcta taagttgacg gcgcgctcag cagagaatca ttgcaagcaa tcggttctcc
     6301 gtccagcgcg atcgacagga ctaggtcttc ttctgtaaag agcgagctgc aaagattgca
     6361 atccccaaca ctacaagtgc gccgatgagt cctgccacaa tggttgaaag agaagtttca
     6421 cccaggaacg gaacagtgta atcggggagg atctgaaatg gcgcatcctg agcgatgcta
     6481 atgaaaccca aatcctgtgc aactcgctcc agtccatccg gattggcaga cgcaaacggc
     6541 gatagtagca caacgatcaa ggagataaag ataccggcta cgacccaacc ccgcccaccc
     6601 tgcgcggaaa cagatttttc atctaataga tcaggacgcg tttgcagaat gaaacccagg
     6661 gcggcaacgg taataagagc ttcaccgagt gcaattaagg cgtggacgcc gagcatcgca
     6721 atcacaaccg tttgcaaatt gctcgtaccg ctcaaccata actgaagggc cgtggcaagg
     6781 gcacccgcca ttacagacag ccaggccgca acaccagcaa cggcgagctt cgcgccacgc
     6841 gaactcccgg acacactacg atacaggcca tagccgattg ccgcggtgag caaacccatg
     6901 tttaaaatat tcgcgcccat tacaagtaag ccgccatcct gaaggatcaa tgcctgcaca
     6961 gcaatgacag ccgtcatcac cagcatgccc gcccatggac ccagcacaat ggccgccaac
     7021 gcacccccaa gcaaatgacc agacgttccg cctgcgaccg ggaagttaat catctgtgcg
     7081 gcgaagataa atgccgccat aatgcccatc aacgggactt gtttttcacc caacgatttg
     7141 ttggttcggg aaatggccat gcttagcgta agagcagtaa tcgcccaaca aatcagggaa
     7201 accacaagat taagaaagcc atccggaatg tgaaggggtt caggtgagtg aggggcaata
     7261 aaaaaatgca tctttttctc cttttctcct ttattgacac tgcgggcaga tgccgaagaa
     7321 cgtcacatga ctatcaatac gcaagtagcc gctggttgat tctaattttc ggtaaaggtt
     7381 ctccaccagc gcgtgatcaa cttcgatttc agaaccacat atgcggcaaa ccacatgatg
     7441 atgatcatca cccgcgatct gataggtgag atgccgtcca tgcgaatggg aggggcgcac
     7501 taagccgttt tgagtcagga actcaagggt tcgatacact gttggctcag tcagacgtgg
     7561 aaaatccttc ttggctcgct tatagatttc ggtcggcgag agatgagtcc cctcgtggtg
     7621 aagaacatgc aggatcgcca accgttgggg tgtcattctg aatccgcgcg cgcggagttg
     7681 gggaacataa tcgacagcac aggacataaa tcaaccttat tgcaactttt tgcaataact
     7741 gcatctagta tacaacaaat gttctatttt tacgatgaga aaagattaag gttcccagaa
     7801 gaatggaatc aaagcgtata gatagggctt attgttcaca tgctcaatac ccaccgccca
     7861 cgtatgccag tccataaaac cattcacttc ggtgccaggg aaatgaacag aatagaaacc
     7921 gccctcatac ggccaggaaa cttcataggt cgcgccacca tgtttgagct cattacaatg
     7981 aagtgtataa ggttgattga acgctttcac taattcgggt actactacat catggaatga
     8041 cccaatctcc tccaggccac ttccgggcgc gggtccccaa tttgcttcaa aggtggtttc
     8101 aaagagaaac tttgcctgat ccgggcggta attgatcaca gtaccatcgc ggaaccgagc
     8161 cacgtctaaa ccgcgctgtg ggctgaccaa cgagcttaac aaaggaccat ctgaatttaa
     8221 cgcagcagtc ttaagtgagt cgatcagtgc taacacctgt gggtcagcac atagatctga
     8281 agcagcagag gtaggaacgc taagagtcag ggttgggatt acagtagacg aaatcgatgt
     8341 cggcaaggat aaaggtggag ttgccgtggg aattaaagtc ccgtttgtgg gcggtaatgt
     8401 gacagcagtc gcaagctggg cgagggtaga agttgcaaca ggggctggtg agccgcaggc
     8461 tgtcagcaaa agcatgctcg caagtaaagc gatcgtactc aatctggatt tcatcttcac
     8521 ctctattgac aattatatcc cttgtcatat aatcaccgaa aattaaataa cgccgcaaac
     8581 ctatcaatca taggtaccca aatttgaact tggaagttaa acgtgcaagc gtgtttaaaa
     8641 gttcagtaat taatggagca agcggtataa ggctacgacg aggacaagta ggtactacac
     8701 gacggcacag agagcaggtg aatcagtgaa agcctgcccg aaacgacact gctgaatgga
     8761 cctcagagcc gcaagagcaa aagtcttcta ttgggttatt cagacacaag tagctcaagc
     8821 cgggaagagc acccgttatc acagctgagt cacgtagcga ctggaagacg ataaactagt
     8881 cgacgaactg actacgtaga gtgaggcagc tttcttaaaa tgaaagcatg cctaacttgg
     8941 gtggcaccac ggacgccaaa gcgaacagcg ttcgtcccat gatgggatga acgctgtttt
     9001 tttgttttga cctcacccca accctctcct caaggagagg gagttaagga gaaatatggt
     9061 tgacatcatt tttcatccac cttgaagagg atagcaatac acagtttgcc attgggttat
     9121 ttattcaaaa aggagaagat catgtctgac caaacccgtt tcatcctgca tgaaagtgac
     9181 ctgccgaaat tctggtacaa catcaatgcc gatagtccag ttccaccaac gcccgtcctg
     9241 catcccggca ccctggagcc tgtcactcca gatttc
//
LOCUS       DASTJP010000504         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1323959, whole genome shotgun sequence.
ACCESSION   DASTJP010000504 DASTJP010000000
VERSION     DASTJP010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323959"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFQ23_14770"
     CDS             <1..226
                     /locus_tag="VFQ23_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907912.1"
                     /translation="LQPIADRIQPRDIAVFHGHIDADKINFIEKWAIKSLVKKPFGDF
                     RDWEAITAWATSIAKTLKEKDTEPSLLSLP"
     gene            302..790
                     /locus_tag="VFQ23_14775"
     CDS             302..790
                     /locus_tag="VFQ23_14775"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9907913.1"
                     /translation="MVNIFKKQHGMNIVGQGGKIILFTLPSLIATIWIHLYLPQIAAL
                     PESLRLIQPVGYVLLVMGLIFWASAVIQLLMGFSQGKLVTTGAYGIARNPIYSSATFF
                     ILPAVALLTLTWVYVVVSVFLYAGVMVFIGTEEKQLKQAFGKEYEGYMTSVDRLVPFK
                     RH"
     gene            complement(799..1215)
                     /locus_tag="VFQ23_14780"
     CDS             complement(799..1215)
                     /locus_tag="VFQ23_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907914.1"
                     /translation="MTARVWDVETGKVIFRFDYPDHVTSVAFSPDGKIIAASGGIPET
                     AKTTLWDALTGKELFSLLGHTDMIGQIVFSPDGTRLATASRDGSARIGSMISASTFMG
                     LGMHPHADTAAFCHGLLIGNYLARLITLARACAPTP"
     gene            complement(1207..1296)
                     /locus_tag="VFQ23_14785"
                     /pseudo
     CDS             complement(1207..1296)
                     /locus_tag="VFQ23_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
     gene            complement(1349..2407)
                     /locus_tag="VFQ23_14790"
     CDS             complement(1349..2407)
                     /locus_tag="VFQ23_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907915.1"
                     /translation="MRVNFKTCFTFGLGLLVTACNPQTVQAPVTTATSAPVISSTPLS
                     IVPLEERLLESINTDEHPLSTQTMIGNHPEEIVFAKGFIWIHLVNGHLVQVDPATNTM
                     VRSIKVDTTNDLNHYCQGLGTDGENIWVCSASGDEDRTIDVVRVDPSSQSVVATFGVG
                     KIFDQLYMPFAQNQIWVLTGDGSKLIGIDVTNNQPNPAIDLGTRCFHLAALGNILYAS
                     CGLDNLVIKIDLEKKEVLARQTLQNHPKFIAAAKSGLWVSQDKSVTRLDPESLNPIIT
                     IPGITPSDINATDTAVWVWEYGKGVLYKIDPATNEVVELIKPDKPFISGGGVLPTSDS
                     IWLTVDENDLLLRLSLND"
     gene            2561..>4865
                     /locus_tag="VFQ23_14795"
     CDS             2561..>4865
                     /locus_tag="VFQ23_14795"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9907916.1"
                     /translation="MSDRPFTPDSFHSFGDLLKYLRRRERLTQLELSILVGYSEAQMT
                     RLEKNQRRPDLTALKALFIPALHLEKEPEWIARLLELAQSVRQEEAPVQGMPPYKGLL
                     FFDESDTHLFFGREALTAQLADRVVNLNMDASSRFLAIVGASGSGKSSLVRAGLAVTL
                     KLAGWDVRTFTPGTDPPRALEMQLELERAKSEAERLLILVDQFEEIFTLCHDESKRAL
                     FVERLLELACKPFRKTTIVIALRADFYSHCAQYPFLRQAVAAEQEYIGQMTLEELRRA
                     IEEPAKRSGWEFEYGLVDVLLHDIGAQGMNQPEPGALPLLSHALLATWERRRGRTLTL
                     DGYRASGGVRSAIAETAESVFTDQLDQAQQEMARNVFLRLTELGQGTEDTRRRATLNE
                     LVRQSQEAHQLRAVLNTLADARLITLNEDSAEVAHEALIREWQRLHEWLTQDREGLLL
                     HRHLTETAQEWKVRDHDAAELYRGARLAQAREWAATHQESLNTLEQAFLTSSVEQEQH
                     DALEREAQRKRELEAAQELADAQSHAASQLRRRAILLAGALVLALVLAGVAIFFGNEA
                     RQNALEAESRELAAAALTNLDDDPERSILLAMQAISTADTLEAENALHHSILASRLRI
                     VLQHDAEVWGVSFSPDGKRIATASQDNTARVWDAETGQLLLTMNGHTAPVNSVVFSHD
                     GERIATASEDSSAKIWDSESGTLLFTLSGHSASIHNIVYNLDGTRLATASNDNTAKVW
                     DADSGEELLTLSNHGNAVFDVAFDPDGN"
BASE COUNT         1227 a         1222 c         1235 g         1181 t
ORIGIN      
        1 gctgcagcca atcgccgatc gcatccagcc tcgggatatt gccgtttttc atggccatat
       61 cgacgcagac aaaatcaact tcatcgaaaa atgggccatc aaaagccttg ttaaaaaacc
      121 cttcggtgac tttcgcgatt gggaggccat cactgcctgg gcgacatcga ttgccaagac
      181 tctgaaagaa aaagataccg aaccatcctt attgagtttg ccttgagaac ccaattgcta
      241 acatagcgac aagaaaaagg aagaaaacat tccagcaaat tcaatccaga ggaagccgat
      301 catggtcaat atcttcaaaa aacaacacgg gatgaacatc gtcgggcagg gtgggaaaat
      361 catccttttt acgctgccgt ctttgattgc caccatatgg atccatcttt atttaccgca
      421 aatcgcagcc ttgcccgaaa gccttcgatt gatccaacct gtgggatatg tattactcgt
      481 gatggggctc atcttttggg cctctgcagt gatccaatta ctgatgggct tttcccaagg
      541 gaaacttgtc accactggcg catacggcat tgcccgaaat cccatctact ccagtgcgac
      601 gttctttatc ctgcctgccg tcgcgctgct gactctgacc tgggtctatg ttgttgtctc
      661 cgtctttttg tatgccggtg tgatggtttt catcggcaca gaggagaagc agctcaagca
      721 ggcctttggc aaggaatatg aaggatatat gacaagtgtg gatcggctgg tcccattcaa
      781 aagacattga tcgtataact atggcgttgg tgcgcaagct cgtgcaaggg tgattaatct
      841 ggctaaatag ttgccaatga gaaggccgtg gcagaatgcc gcagtatctg catgtggatg
      901 catgcccaac cccataaatg tagatgcact gatcatgctg cctattctgg cgctgccatc
      961 gcgactggca gtcgccaggc gcgtgccatc ggggctgaag acaatttgcc caatcatatc
     1021 cgtatgaccg agcagcgaga acagttcttt cccagtaagg gcatcccaga gtgtcgtttt
     1081 ggctgtttca ggaattccac ctgaggcagc gataattttg ccatccggac tgaaggctac
     1141 actcgttacg tgatctgggt agtcgaacct aaagatgacc ttgccggtct caacatccca
     1201 tactcttgcg gtcaaatcct cactggcagt agagatccgg gaaccgtccg agctaaatgc
     1261 aacgccgagc acaggatatt cgtggcccaa taaggtttgt aattccctgc cggtaaatca
     1321 ttctcaggcg gcaatcaaat cccatgcttc aatcattgag actaagccga agcaatagat
     1381 cgttctcatc gacagtcagc cagattgagt cagacgtggg tagcacacct cctccactaa
     1441 taaagggctt atccggtttg atcagttcca caacttcatt cgtggccgga tcaattttgt
     1501 ataagacgcc ttttccatat tcccataccc aaactgcggt gtccgttgca ttaatatcac
     1561 taggcgttat tccaggaatc gtaataattg gattcagtga ttcagggtcg agacgagtga
     1621 ctgacttatc ctgagatacc cataatccac tcttcgccgc ggcaataaat tttgggtgat
     1681 tttgtagcgt ttgtcgcgct aagacttcct ttttctctag atctatcttg attacaagat
     1741 tatccaagcc acatgatgca taaaggatgt tacccaatgc ggctaaatga aaacaacggg
     1801 ttccaagatc tatagctgga ttaggctggt tgtttgttac atctattcca atcaatttgc
     1861 ttccatcccc tgtgagcacc cagatttggt tttgagcaaa gggcatgtac aactggtcaa
     1921 aaatctttcc cacaccaaaa gttgcgacaa cactctgcga tgatggatca acgcggacaa
     1981 catcaatcgt ccggtcttca tcaccacttg ccgaacatac ccagatattt tctccgtccg
     2041 taccaagccc ttgacagtag tgatttagat cattagttgt atccactttg atggaacgta
     2101 ccattgtatt ggtggcagga tcaacctgaa ccaaatgacc gttgactaga tggatccaga
     2161 tgaaaccttt tgcaaataca atttcttccg ggtgattacc gatcatcgtt tgggtagaaa
     2221 gtggatgttc atcggtgtta atcgattcca gtagccgctc ctccaatggc acaatggata
     2281 agggagtcga agaaattact ggtgctgatg tcgccgttgt tacaggcgcc tgcacggtct
     2341 gagggttgca tgcggtaact agcaagccaa gcccaaaggt aaagcatgtt ttgaagttaa
     2401 ctctcatatt cgttctcctg ggtaatttcg attattttca cttatgcagg ttaacattcc
     2461 ttcgaagata aaacctgaca tttcctccca aattgtgtac atgaactacc agagtcttga
     2521 atttgctgct tcccggctat aaaataaaca caataagatt atgtctgatc gacctttcac
     2581 tccggattcc ttccactcgt ttggtgattt attgaaatac ttgcggcggc gtgaacgcct
     2641 tacgcagctc gagctctcga ttttggtcgg ttacagtgaa gcacagatga cccgcctgga
     2701 aaagaaccaa cggcgacccg acctcaccgc gctcaaggca ttgttcatcc ccgcgttgca
     2761 tctcgaaaaa gagccagagt ggatcgcacg tttgttagag cttgcccaat cggtccggca
     2821 ggaggaggca cccgtgcagg gtatgcctcc atataaaggc ttattgttct ttgacgaatc
     2881 cgatacgcac cttttctttg gacgggaagc attaacagcg cagcttgcgg accgcgtcgt
     2941 aaacttaaat atggatgcat cttcccgttt cctggctatc gtcggtgcgt ctggaagcgg
     3001 aaaatcgtcg cttgtgcgcg ctggtctggc ggttacgctc aaacttgcag ggtgggatgt
     3061 ccgaaccttt acacccggta ccgatccacc aagagcgttg gagatgcagc ttgagttgga
     3121 acgagccaag tctgaggcgg agcggctgtt gatccttgtg gatcaatttg aagagatatt
     3181 cacgttgtgc catgacgaat caaaacgcgc cttattcgtt gagaggttgc tcgagctcgc
     3241 gtgcaagcca ttcaggaaaa caacgatcgt cattgcctta cgcgcagatt tctattcgca
     3301 ctgtgcacag tatccgttcc tgcgccaggc ggtcgctgca gagcaggaat atatcggcca
     3361 gatgacactt gaagaattgc gccgtgcgat cgaagaaccc gccaaacgca gtggatggga
     3421 gtttgaatat gggctggtag atgtattact gcacgacatc ggtgcacaag ggatgaacca
     3481 gcccgaacct ggcgcgcttc cgttactgtc acacgcgttg cttgccacat gggaacgccg
     3541 acgcgggcgg acgctgacgc tggatgggta tcgtgcttcg ggcggcgtgc gcagcgcaat
     3601 tgcagagaca gccgagagcg tgttcacaga ccaactcgat caggcacaac aggaaatggc
     3661 gcgcaatgtc ttcctgcgtc tcaccgagtt gggccagggt acggaggata cacgccgccg
     3721 cgcaacgctg aatgaattgg tgcgccagtc gcaggaggcg catcaactga gggcagtgct
     3781 gaacacactg gcagatgcca ggttgatcac actcaacgag gatagtgctg aagtcgcgca
     3841 tgaagcgctg atccgtgaat ggcaaagatt gcatgaatgg ctcacacagg accgcgaggg
     3901 gttgctcctc catcgtcatc tcacagagac cgcgcaggaa tggaaagtgc gcgatcatga
     3961 tgcagctgaa ctttatcgtg gagcacgtct ggcgcaggct cgtgaatggg cagcaacgca
     4021 ccaggagagt ctaaataccc tggaacaggc ctttctcaca tcatccgtcg agcaggaaca
     4081 acacgatgct ttggagcgcg aggctcagcg caagcgcgaa ctggaagctg cgcaggaact
     4141 cgctgacgcg caaagccacg ctgccagtca attgagacga cgtgcaatct tattggctgg
     4201 ggccctggtt cttgcactgg tgttggctgg tgtggcgatc tttttcggaa atgaggcaag
     4261 gcagaacgct ctggaagcag aatccaggga actggcagcc gcggcactca ctaatctcga
     4321 cgacgatccc gagagaagca tcctgcttgc catgcaggct atctcaactg ccgacacgct
     4381 ggaggctgaa aatgccctgc atcattccat acttgcctca cggttacgaa ttgtccttca
     4441 acatgatgct gaagtgtggg gcgtcagttt cagcccggat ggaaaacgta ttgctaccgc
     4501 gagccaggac aacaccgcaa gagtctggga tgctgagacc ggacaactat tgctgacaat
     4561 gaacggtcat accgcccctg taaacagtgt tgtatttagt cacgatggag aacgcattgc
     4621 caccgcgagc gaagactcgt ccgcaaagat ttgggattcg gagtcgggta cattgctgtt
     4681 cacattaagt ggtcacagcg cgtccataca taacattgtc tacaatctcg atggaacgcg
     4741 cctggccaca gcgagtaacg acaacacggc aaaggtctgg gatgcagaca gcggagagga
     4801 gttgctcact ctttcaaatc atggaaacgc cgttttcgat gttgcgtttg atccagatgg
     4861 aaacc
//
LOCUS       DASTJP010000505         5761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_967344, whole genome shotgun sequence.
ACCESSION   DASTJP010000505 DASTJP010000000
VERSION     DASTJP010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967344"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..556)
                     /locus_tag="VFQ23_14800"
     CDS             complement(143..556)
                     /locus_tag="VFQ23_14800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907917.1"
                     /translation="MSKIALSAKAHWGYPERWMQIWTPLLTYRAEYFDENECWAAVLD
                     NSPIGFYTLHEKNGNAWIEDLWVQPEYIGKGIGRQLFFDAISRARQRGHKSLQLEADP
                     HAVGFYEKMGMYKIGERHSEVDGQPRILPIMEMKL"
     gene            complement(717..1823)
                     /locus_tag="VFQ23_14805"
     CDS             complement(717..1823)
                     /locus_tag="VFQ23_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9907918.1"
                     /translation="MTQPRFEKLNTSLQTSDLDAVILNPGPTLTYLTGLHFHLMERPV
                     VFLFARDQDPAIVLPELELQKVASLPYNLHVFSYPENPAEWDNAFRKAVQALGLDGKQ
                     IGVEPRQLRLLEFRHVKNGAPEADFPDASNILSGLRLRKDRAEVDAMRQAVKIAQDAL
                     EATIPSIKIGMTEREVASELVVQLLKHGSDSEMPFAPIVSAGPNSANPHASPTDRELQ
                     TGDLLVVDWGASHAGYISDLTRTFAVGNVDEEYEKIHRIVQESNAAGRAASKPGVPCA
                     NVDNAARDVIEKAGYGVYFTHRTGHGIGMEGHEEPYMRGDNRQLLEPGMAFTVEPGIY
                     LPGRNGVRIEDNVVITEDGADVLSDMPREMRVVG"
     gene            complement(1842..2654)
                     /locus_tag="VFQ23_14810"
     CDS             complement(1842..2654)
                     /locus_tag="VFQ23_14810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9907919.1"
                     /translation="MAITLQGKVVLITGASSGFGEDAARLFAREGCKVVLSARRLEKL
                     QTLASDIQDSGGEALAVPVDVTERAEIELMVQTVLDLYRKIDILFNNAGFGAVDWFEK
                     FKPERDIETMIRVNLIGTMLVTRTVLPHMLSRREGHIINMASVAGLIAAPTISTYSAS
                     KYGVRAFTDSLRREVSPFGIRVSGIYPGPAKTEFGQKLERTSSRETLRKIRYPHLTSE
                     YVASQVVEVAKRPRRTLVIPGWFRVITTFDTLFPVIVDWILYWFSKIKHELD"
     gene            complement(2803..5283)
                     /gene="lon"
                     /locus_tag="VFQ23_14815"
     CDS             complement(2803..5283)
                     /gene="lon"
                     /locus_tag="VFQ23_14815"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9907920.1"
                     /translation="MSHPKEWNEEEDNFDLTLSQRTEELYRIPNRDPNAEGLIEAAVL
                     PLRDLVVFPRMVSPIFVGREASLLAVQEAQNKEQTVIGLTQTDADLDNPGVGDFLPVG
                     VELAVGRLLQMPDGSHSALVQGRRRVEVVEFIRTKPYLMVRARVVVEPQVADRQTQAL
                     MRSALDLFDRCVQLDRSIPEEAHLFAMNISEPGWLADMLATSLALNYEAKQSLLLLLD
                     VRGRLQALNKILAQEVDVLELEDEIHSRVQNEVDKSQREFYLREQMRQIQNELGEGDI
                     FTRDVTDLKKKVEAANLPDEPKLVALKELDRLNQMPPMAPEMGIIRTYIDWIVELPWI
                     TSSPDNLDVKNAAKVLDRDHYGLKKAKERILEYIAVRSLKPKKERQPILCFVGPPGVG
                     KTSLGRSIAESLGRKFVRVSLGGVRDEAEIRGHRRTYIGALPGRIIQTMKRAGTANPL
                     FMLDEIDKLYSDFRGDPASAMLEVLDPEQNYAFSDHYLELPFDLSKVMFVTTANNLGT
                     IPLPLLDRMEVIEFPGYIEEEKLEIANRYLIPRQIEENGVDELNLTFEPEAVRRIIRE
                     YTYEAGVRNLEREIGRICRKIARMKAEGRKYPIIIIAESIDKFLGPQQVFQTEAERSD
                     EVGVVTSLAWTENGGEIMPVEVAVLEGKGGLQMTGQMGEVMQESGQAALTYIKSRGPQ
                     LNIDLDVFERMDIHVHMPEGAIPKDGPSAGITMATAIISALTGRPVFKHVGMTGEITL
                     RGRVLPIGGVREKVLAAHRAGLKTVILPEKNLKDLVDLPKTAKSELKIIPVKHMDEVL
                     QIALAREATIEPPRPKKPLKEETQDDSE"
     gene            complement(5270..>5761)
                     /locus_tag="VFQ23_14820"
     CDS             complement(5270..>5761)
                     /locus_tag="VFQ23_14820"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9907921.1"
                     /translation="IQKGFAQVHWPARFEVVRRDPPVILDSAHNQDSFARLRETLEEH
                     FPDKKVYLIFGASEDKNIPGMFAEMQPKVNKLIATRADHPRALEPEVIIELADQAGLK
                     SEAVSPVESAFARALDLSEKDGSIVLSAGSMFVTAEVMKAWNKLALTRPEVSGEGRIH
                     ESP"
BASE COUNT         1368 a         1569 c         1385 g         1439 t
ORIGIN      
        1 ccaaatggac tctgtgatcg gcttaatttc ggcgatcgat caagcagttg tttttgatga
       61 aacctatacc ccgccagttt gtgcggctac atagagcttg aagccatcag aagctgagaa
      121 cgaggatgat ctcaacgacg gatcatagtt tcatttccat gatcggtaat atacgcggtt
      181 gaccatctac ttcagaatgc cgctcaccga ttttgtacat ccccattttc tcgtaaaaac
      241 caactgcatg cgggtcagct tcaagctgca acgatttatg tcccctttgg cgagcacgcg
      301 agatagcatc gaaaaataac tgcctgccga tacctttacc tatgtattca ggctgtaccc
      361 agagatcttc tatccacgcg ttaccattct tctcgtgcag agtgtaaaac ccaatcggac
      421 tgttatccag gacggccgcc cagcattcgt tttcatcgaa atactctgca cgatacgtca
      481 gcaacggagt ccagatttgc atccagcgct ccgggtaacc ccaatgggct ttggctgaca
      541 aagcaatttt gctcattaca ttagcttctt ccggaagaac acggcgaatc aaaatcatgt
      601 cttgagcaga tcgttttgat gaagcatttc tctaatactc cattctatcg gagtacgtct
      661 ttgccagagt tgcgcatcaa gcaaatccgg gtatttgata attacatcat ccaaaattat
      721 ccaacgactc gcatctcacg cggcatatcg gataatacat cggcgccgtc ctcggtaatc
      781 acaacattat cctcaatccg cacgccgttt cggccgggaa ggtaaattcc gggctcaacg
      841 gtaaatgcca tgccaggctc aagcaattgc ctgttgtcac cgcgcatgta cggctcttca
      901 tgaccttcca ttccgattcc atggccagtt cgatgagtga agtaaacgcc atagccggct
      961 ttctcgatca cgtctcgtgc agcattatcc acgttggcac agggaacacc gggtttcgaa
     1021 gcagcgcgac ccgcggcatt tgactcttga acaattctgt gaatcttttc gtattcttcg
     1081 tcgacgttcc ctaccgcgaa ggtgcgcgtc aggtcagaga tataacctgc atgtgaagcg
     1141 ccccaatcca cgacgagtaa gtcacccgtc tgcagctcgc gatcagtggg tgaggcgtgt
     1201 ggattcgcgg agttgggtcc cgcagagacg atcggtgcaa atggcatttc agagtctgaa
     1261 ccatgtttga gtaattgcac gaccagttcc gatgcgacct ctctttcggt cattccgatt
     1321 ttgatggatg gaatcgtcgc ctccagcgca tcctgagcga tcttcaccgc ctggcgcatg
     1381 gcgtccactt ctgctcgatc tttcctcaaa cgcagacccg ataagatatt gcttgcatca
     1441 ggaaaatccg cttcgggcgc gccgttcttt acatgccgaa attccagcaa acgcagttgc
     1501 cgcggctcaa caccaatttg ctttccatcc agacccagcg cctgcacggc ctttcgaaac
     1561 gcgttatccc attctgccgg gttttccgga taagaaaaga catgaaggtt ataaggcagg
     1621 gatgctacct tctgcaattc caattcaggc agaacaatgg caggatcctg gtccctcgcg
     1681 aacaggaaga caacaggtcg ttccattaaa tggaagtgca agccagtgag atacgtcagc
     1741 gtggggccgg ggttgagaat aacggcatcc aggtcggacg tctgaagtga ggtgttgagt
     1801 ttttcaaaac gtggttgagt catgtgcagc tcctgaaaag tctaatccaa ctcatgtttt
     1861 attttcgaaa accaatataa aatccaatca acgataacag ggaaaagtgt atcaaatgtt
     1921 gtgataaccc ggaaccagcc tgggatcacg agagttcgac gtgggcgctt tgctacttcc
     1981 acaacctggc ttgctacata ctcagacgta agatgtggat aacgaatttt tctaagcgtt
     2041 tcacgcgaag aagtccgttc aagtttttga ccgaactcgg tctttgcagg gccagggtaa
     2101 atccccgaga cacgaatccc aaacggtgag acttcgcgcc ggagcgagtc agtgaaggcg
     2161 cgcacaccat acttgcttgc cgaataggtc gaaatcgtcg gcgcggcgat gagacccgcg
     2221 acagaagcca tattgataat atgcccttca cgacgagaca gcatatgcgg gagaaccgtc
     2281 cgcgtgacta acatggtgcc aatcaggttg actcgaatca tcgtttcgat atcacgttcc
     2341 ggcttgaatt tttcgaacca gtcgacggcg ccgaatcctg cattattaaa aagaatgtca
     2401 atctttctgt acagatcgag tacggtctgc accatcaatt cgatttctgc ccgctccgtc
     2461 acatccaccg gcacggcaag ggcctcacca cctgaatcct ggatgtcaga cgccagcgtt
     2521 tgcagctttt caagacgacg cgctgaaagc actactttgc agccttccct cgcgaagaga
     2581 cgtgcagcat cttcaccgaa tcccgatgat gcgcccgtaa tgaggacaac cttgccttgg
     2641 agagtgatcg ccataatttc gatagagaaa ggggttcacg aaagacgcaa agcgcgagtg
     2701 cgttttgcgt ctttgattcg agtcaatttt accgccgagc gtttgtgaat ctacggggct
     2761 atttcatatt aaattttagt tccagcaaag gcagtttgcc gcttattcac tatcgtcttg
     2821 tgtttcttct ttgagtggct tcttcggccg gggcggctca atcgtcgctt cacgggcgag
     2881 agcgatctgc aacacctcat ccatatgctt gaccgggatg atcttcaatt cagatttcgc
     2941 agtcttgggt aaatcaacca aatcttttag attcttttcg ggcaaaatta cagtcttcaa
     3001 gccagctcga tgcgctgcta aaactttttc gcgcacgccg ccaattggca gcacacgtcc
     3061 tcgcagcgtg atctcaccgg tcataccaac atgcttgaag accggtcgac ctgtcaacgc
     3121 cgagataatg gcggtcgcca tcgtaatccc ggcagatggt ccatcctttg gaatggcacc
     3181 ttcaggcata tggacgtgaa tatccatgcg ctcgaaaacg tcgaggtcaa tattgagctg
     3241 tgggccgcgc gacttgatat aggtcagcgc agcttgccca gattcctgca tcacttcacc
     3301 catctgaccg gtcatttgca ggccgccttt gccttcgagc acggccactt ccacaggcat
     3361 gatctcgcca ccgttttccg tccaggcaag gctggttacg actcccactt catcgctgcg
     3421 ttctgcttca gtttgaaaga cctgttgagg gcccaggaac ttatcaatcg attcggcaat
     3481 gatgatgatg ggatactttc tgccctccgc tttcatgcgc gcgattttgc gacagatgcg
     3541 cccgatctcg cgctccaaat tgcggactcc tgcctcgtag gtgtactctc gaataatacg
     3601 ccgcaccgcc tcaggctcga atgttagatt gagttcatcg acgccatttt cttcaatctg
     3661 acgaggaatc aaataacgat tggcgatttc taatttctct tcttcgatgt agccagggaa
     3721 ctcgatcact tccatacggt cgagaagtgg caacgggatc gttcctagat tattggcggt
     3781 tgttacaaac atgacctttg agagatcaaa tggcagttcg agataatgat cgctgaacgc
     3841 atagttctgc tcaggatcca gcacttccaa catggcggac gccggatccc cgcggaagtc
     3901 ggaatacagc ttgtcgatct catccagcat gaaaagcgga ttggctgtac cggcgcgctt
     3961 catcgtttga ataattcgtc cgggcagcgc accaatatag gttcttcgat gcccgcgaat
     4021 ttcagcttca tcacgcaccc cacccagtga gacgcgcacg aacttgcgcc caagcgactc
     4081 ggcaatcgag cgccctaagg acgtttttcc aacaccggga ggaccaacaa aacaaaggat
     4141 tggctgtcgt tccttctttg gcttcagtga acggaccgcg atgtattcaa ggatgcgttc
     4201 cttggctttt ttgagaccgt aatggtcacg gtccaatacc ttcgctgcgt ttttcacatc
     4261 cagattgtct ggtgaggaag tgatccacgg caattcaacg atccagtcaa tgtaggtgcg
     4321 gatgataccc atctctggcg ccatgggcgg catttggttg agtcgatcaa gctccttgag
     4381 cgcaaccagc tttggttcat cgggaagatt tgcggcttcc acttttttct tgagatcggt
     4441 gacatccctc gtaaagatat caccttcgcc caactcgttc tggatctgcc gcatttgctc
     4501 acgcagataa aactctcgct gactcttatc cacttcgttt tgcacgcggg aatgaatttc
     4561 atcttccagt tcgagaacat ccacttcctg cgccaggatt ttgttcaatg cctgcaaccg
     4621 tccacgcaca tccaaaagca gcagtaagct ttgcttggct tcgtaattaa gcgccagtga
     4681 ggtggccagc atatctgcca gccagcctgg ctcggagata ttcatcgcga acaaatgggc
     4741 ttcttcagga atcgagcggt cgagttgcac gcaccgatcg aagaggtcca aagcggaacg
     4801 catcagcgcc tgagtctgac gatcagcaac ctggggttcc acgacgacgc gggcgcgcac
     4861 catcagatag ggtttcgtgc gaatgaattc cactacttcc acacggcggc gtccctgcac
     4921 cagcgcggaa tgagagccgt ctggcatttg caacaggcgc cccacagcca gttccacccc
     4981 aaccggcaga aaatcgccga cgccgggatt atccaaatcg gcatccgtct gtgtgagccc
     5041 gatcacagtt tgttctttgt tttgtgcctc ttgtacagcc agcagtgaag cctcacgccc
     5101 gacaaagatc ggcgagacca tacgcgggaa aaccaccaga tcgcgaagtg gtaaaacggc
     5161 tgcctcaatc aaaccttctg cattggggtc gcgattggga atacgatata actcttccgt
     5221 ccgttgcgaa agggttaaat caaaattatc ttcttcttca ttccattctt tagggtgact
     5281 catgtatcct cccctcacct gacacttcgg gtcgggtaag ggctaattta ttccatgctt
     5341 tcattacttc cgcagtcaca aacatcgagc ccgcggagag gacaatgcta ccatcttttt
     5401 cggataaatc cagcgcgcgg gcaaaggcgg attccaccgg actcaccgcc tctgacttca
     5461 gccctgcctg gtcggccaac tcgatgatta cctccggctc gagcgcacgt gggtgatctg
     5521 cacgcgttgc aatgagttta ttgacctttg gctgcatctc cgcgaacata cccggaatgt
     5581 tcttatcctc cgaggcgcca aagatgagat agaccttttt atccggaaaa tgttcttcca
     5641 atgtttcgcg caatcgtgcg aaggagtcct ggttatgtgc tgagtccagt atcaccgggg
     5701 gatcgcgtcg cacaacttcg aagcgagcag gccaatggac ctgggcaaat cctttttgaa
     5761 t
//
LOCUS       DASTJP010000506        10408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_862724, whole genome shotgun sequence.
ACCESSION   DASTJP010000506 DASTJP010000000
VERSION     DASTJP010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862724"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFQ23_14825"
     CDS             <1..248
                     /locus_tag="VFQ23_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET9907922.1"
                     /translation="SHELLFDEDEIDSDVLVTGTSKEAREETIKLVEAAGLEGWDAGP
                     IENSVVIEGLVSVLININKRYGSTHSGVKITGVVKKS"
     gene            268..1062
                     /gene="cofE"
                     /locus_tag="VFQ23_14830"
     CDS             268..1062
                     /gene="cofE"
                     /locus_tag="VFQ23_14830"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HET9907923.1"
                     /translation="MSLTLTPLSRIPFIRRDDNLADILVKSLSDSNIVVQDDDILVLA
                     QKIVSKAEGRSVNLNTVSPSQRAQELALETNKDPRLVELILQESNEVLRTRPGVIIVE
                     HRLGFVCANAGIDHSNVGPSTTHEESAEGTAVREDTVLLLPENPDGSALALHRQMREK
                     TGKQIGILIIDSHGRAWRNGTVGIAIGMACLPGIEDLRGKPDLFGYTLQVTQVGVADE
                     LAAAASLVMGQAAEGTPAVHVRGFPYPLRESSLRELIRPKDQDLFR"
     gene            1116..2087
                     /locus_tag="VFQ23_14835"
     CDS             1116..2087
                     /locus_tag="VFQ23_14835"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET9907924.1"
                     /translation="MSNSKSDRLLDSVLGAIGNTPLVELSRITRGLDGRILAKLEYLN
                     PGFSKKDRIALQMIEEAEAEGKLQPGQTVVELTSGNTGTGLSIVCAVKGYRFVAVMSK
                     GNSTERARMMKALGAEVILIDQLPESKPGQVSGGDLALVEEAAQRITIERNAFRADQF
                     HLESSARAHYLHTVPELLQQSNGEIDAFCDFAGTGSSFGGCAAAFKEHNQNILCYIVE
                     PAGAAILGGQPVSNPNHRIQGGGYSMTELSLINKTHIDGYLQVTDEEAMEFARRLAKD
                     EGIFAGFSSGANVAAAIQLLNTTCKGKTIAVLLSDSGLKYLSTDLWP"
     gene            2228..2620
                     /locus_tag="VFQ23_14840"
     CDS             2228..2620
                     /locus_tag="VFQ23_14840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET9907925.1"
                     /translation="MNFPEGFFDLLNDQSKALLYLATIMPDGSPQVTPVWFDTDGEHI
                     LINTNEGRVKDRNMKARPRVAMTIQDPNDPYRYLGVRGEVVNYTTTGADEHINKLSLK
                     YDDEPWTYRESQRRIIFTIKPLHFDEHN"
     gene            2604..3215
                     /locus_tag="VFQ23_14845"
     CDS             2604..3215
                     /locus_tag="VFQ23_14845"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9907926.1"
                     /translation="MTNTTDLQDFLRTRRSVRRFKPDPVPIAVIQTILTTATYAPSAH
                     HRQPWRFVVVTDIGVKARLADAMAIDFERDLARDQLPVEKIQAQIKRSKERITSAPLA
                     ILLCLDMSEMDSYQDEKRNKAEYMMAVQSVATAGLQLLLAAHAEGLGGVWACWPLFAQ
                     ATIQSTLNLSEAWEPQGMFFLGYYETAPEVRIRKPLQDIVVFR"
     gene            3212..4168
                     /gene="cofD"
                     /locus_tag="VFQ23_14850"
     CDS             3212..4168
                     /gene="cofD"
                     /locus_tag="VFQ23_14850"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HET9907927.1"
                     /translation="MKIVALAGGVGGAKLAHGLAQILKPEELTVIVNTGDDFEHYGLY
                     ICPDLDTVCYTLAGIANPETGWGRAGESWNAMENLSKLGGLDWFRLGDKDLGTHLERT
                     RRLKEGFSLSQITRDFCKAWGIQFAVLPMSDQPVRTYVETDEGELAFQEYFVHRRCEP
                     RVKGFWFDGVERAEPARGALEAIQAADAIVICPSNPWVSIDPILKVLSLTPAPLPLGE
                     ERSSEGKRVYAVSPIVGGQAIKGPAAKMYTELGIEPSAFAVANHYRGLITDFVLDQID
                     EQLNESVKGLSMQTHVTNTLMKSHEDRKRLATDLLKFIGSDL"
     gene            4165..4776
                     /gene="cofC"
                     /locus_tag="VFQ23_14855"
     CDS             4165..4776
                     /gene="cofC"
                     /locus_tag="VFQ23_14855"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HET9907928.1"
                     /translation="MTLWAIVPVKPLRRGKSRLSGILTEDERADLNRKLLQHTLETLN
                     ELKEIEEVLVVSRDPQVLTIARNYGARTVREDGQPDLNTALRRATVIAEVYATRGVLV
                     LPADLPLISREDARALVERAVDPPVVVIAPDRHEKGTNALLISPAGLIEYDFGENSFQ
                     RHCNLVKKAGVRLEVVNLPSLGLDLDSPEDFEIIKNLESTISI"
     gene            4795..5793
                     /locus_tag="VFQ23_14860"
     CDS             4795..5793
                     /locus_tag="VFQ23_14860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9907929.1"
                     /translation="MSQRVALYLQDSHDLRDGLEYARYAEEKGFEAVWQAESRLVRDA
                     IVPMAGYAAVTNRIKVGSGVINNWTRNIGLLAATFLTLDDLAPNRVICGIGAWWDPLA
                     KNVGIERKKPLLAMRETIEVLRRLLNMERVTFHGEFHHVDGIELDVVHGRREPRNIPI
                     MIGATGDLMMELTGEIADGAVLNYCVPPDYNDNALELLEKGLKKSGRKLEDFDRPQLI
                     VCSVDEDHDKAIEYTKELLCQYLAQQPHIAKASRVSQSVINEIQSILGWPATKEQINQ
                     AKHLVPDELVHNITASGTPTEAKAKVEEYKKRGCTCPILYPVGGNFKLLIDTFAQA"
     gene            complement(5790..6320)
                     /locus_tag="VFQ23_14865"
     CDS             complement(5790..6320)
                     /locus_tag="VFQ23_14865"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET9907930.1"
                     /translation="MNRAKYNLHNKFAEVTVTNTLETQIGSMLLERGWKLALAESCTG
                     GLVGSRVTDVSGSSEYFLGGVVSYSNEAKVNILGVSWDTLNTTGAVSRDTVIEMARGA
                     RKLMNADIAVSVSGIAGPGGGTAEKPVGTTWIGLAAHDGEWSRVFEFSGDRVQNKSAA
                     ADAALLMLLDYLQNKL"
     gene            6342..8447
                     /locus_tag="VFQ23_14870"
     CDS             6342..8447
                     /locus_tag="VFQ23_14870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9907931.1"
                     /translation="MADWAGKTLGKVHIDSLVARGGMAEVYTGTHESFGQVAVKVMRG
                     LLERDSDQLARFQREAEVVEELRHPNIVQMFDYTVVDETPCLVMEYIPGPSLHVYLKH
                     LHDNKQRLPIGIVGHILTSMASALDYAHSKGMVHRDIKPANVLLRSRSEPVELNKPLP
                     ADVEPVLTDFGLVRLLDSTMHTTAGSVSGTPTYMSPEQARGEKVDKRTDIYSFGIMLY
                     EMLAGEVPFQSDTTFGMLMKHINDPPAPIKGISKDLQALLDRALAKDPSLRYETAGDL
                     ANEFMALFNGQTISPGTLHIAEMARRAAEASKRGQTSDGVRQGWSPLTIGLGIVAVLG
                     LAFIISTFFSPRQNTLTVTPTPTRDPNFDVGRLRFGDAGDIMDELVISLTNGVQPPAD
                     GTHFEGWLMNDDGTVTRPLGPVIFNDAGVGRLEFRNLGSQNILATFNQLIISAEVGNI
                     EDGAVVTQPSTDIRYSSIFPPEALIPIRRILVSDPDTPDEEALIQGLWFYGAQYINTS
                     INGDSENDPEYISLVAALDAEDEPTFRKRTEEIINQIVGDASEQFLDYDEDGTVEYYL
                     GDAYGSLPAGDRPGYIQATEMNAQAAADAADSTGNIRLFSNNTQICMENMNTWTNELL
                     QLALQLVDMPFGPEMRSTVDRMAELGKWLYDGNDVNNDSQIDPIPGECGITIAYEEAY
                     SMADMLIYPGPDRLQPTGK"
     gene            complement(8500..9198)
                     /locus_tag="VFQ23_14875"
     CDS             complement(8500..9198)
                     /locus_tag="VFQ23_14875"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HET9907932.1"
                     /translation="MNEQPNAPMLPDRPQPMFQTWINALTKPSEETYVQIANSPQAKT
                     GTALLWVFVGSLIQSFIVFLLQGVLMNRMMQGSGFESPMSTSLIGVLCGAPVAAAISV
                     VLFASFTGIVQWIAGLFGGRGSFEKLVYVLAAITVPFTLVSALFTLLSAIPFVGFCFG
                     LVSLLAALYVLYLEVMAVKAVNQFDWGKAAASLLLPGVVIFCCVLVAIGGLFSILGPQ
                     MQDIFNQINQSITP"
     gene            9363..>10408
                     /gene="uvrC"
                     /locus_tag="VFQ23_14880"
     CDS             9363..>10408
                     /gene="uvrC"
                     /locus_tag="VFQ23_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET9907933.1"
                     /translation="MEISEKLQGILATLPAKPGCYLMKNNEGKIIYAGKAISLKNRVR
                     SYFHADASHDTKTRRLVREIVDIEWIVVGSELEALILEMNLIKKHRPKYNVRLKDDKR
                     YPYIKIHWADPFPKVTVTRQMVEDGSRYFGPYTSAWAVYQTLDVLRRIFPYLTCDRNI
                     TGLDQRACLYYDIKLCTAPCIGAVNQAQYRQTISDLMDFLSGHSDDIVARLQADMEKA
                     SEEMRFERAAALRDQLKAIQAIIERQKIVFASDYVDSDVLAMARANGEACVQVFFIRG
                     GKLIGREYFILEGTEDEADSEIMEQFVTQFYTEAATVPPQVMLPEEIEEARIISQWLR
                     SRRGGEKVEFFVPK"
BASE COUNT         2783 a         2561 c         2640 g         2424 t
ORIGIN      
        1 tttcgcatga actactcttt gacgaagatg aaatcgactc agatgtttta gtgaccggca
       61 caagtaagga agcgcgtgag gaaaccataa agctggttga ggcagcgggg ctcgaaggtt
      121 gggatgcagg ccccatcgaa aactcggttg tgatcgaggg tctggtcagt gttctgatca
      181 acatcaataa gcgctatggc tcaacgcatt ctggtgtaaa gatcacaggc gtcgtcaaaa
      241 aatcataatc cattcctcta ctaatgcatg tctcttaccc ttacccctct cagtcgaatt
      301 ccgtttatcc gccgagacga caacttggcg gatattcttg tcaaatcact gtcagactcc
      361 aatatcgttg ttcaggatga tgatatcctg gtcctcgcac aaaagattgt ttcaaaagct
      421 gaaggacgat ctgtaaatct caacacggtg agtccctctc aacgcgcaca agagctggcg
      481 cttgaaacaa acaaggaccc acgccttgtc gaactaattc ttcaagaaag caatgaggta
      541 ctgcggacgc gtccgggagt aattattgtc gaacaccggc tcggcttcgt ctgtgcgaat
      601 gcaggtattg atcattccaa tgttggacca tcaaccactc atgaggagtc tgcagaaggc
      661 acagcggtga gggaagatac ggtgctattg ctccctgaaa accccgatgg ctccgctcta
      721 gcactccacc ggcaaatgcg ggaaaaaacc ggaaaacaaa tcggcattct catcattgac
      781 tcccatgggc gcgcctggcg caatggcacg gtagggattg ccatcggaat ggcatgtcta
      841 ccgggcattg aagatttacg tggcaaacct gatctctttg gctatacgct acaggtcaca
      901 caagtgggcg tggcagatga attggcagcc gccgcttcac ttgtgatggg acaggcggcg
      961 gagggaacgc ctgcggtcca tgtacgaggg tttccgtatc ccctccgaga gagttcgctc
     1021 cgagaattaa tcagaccgaa agaccaggat ttgttccgat aacagcaatg gtgtatgtag
     1081 tttttcattc tctgcactaa agggagatag acataatgag caatagcaaa tcagatcggc
     1141 tgttggattc ggtattggga gccatcggca atactccact tgtagagctc tctcgcatca
     1201 ctcgcggact tgacggccgc atccttgcca aattggaata tctcaaccct ggtttttcca
     1261 agaaagatcg cattgccctg caaatgatcg aggaagccga agcggaaggc aagctccagc
     1321 ccggtcaaac cgttgtcgaa ttgaccagcg gcaacaccgg gacaggactc tccattgtct
     1381 gcgcagtgaa aggctatcgg ttcgttgcgg tcatgtcgaa aggcaattcg accgagcgag
     1441 cccgcatgat gaaagccctg ggagcggagg ttattctcat tgatcaactg cctgaatcca
     1501 agccaggaca ggtttccggg ggagatttag cactggtaga agaagcggcg caacgaatca
     1561 caatagagcg aaatgcattc cgcgcggatc aatttcatct ggagagcagc gcgcgtgctc
     1621 attacttgca taccgttcca gaactacttc aacaatcaaa cggcgaaatc gatgccttct
     1681 gcgattttgc gggcactggt agctcctttg ggggctgtgc ggcggcattc aaggaacata
     1741 accagaacat cctgtgttac atcgtggagc ctgcgggggc agctatcctg ggcggccagc
     1801 ctgtatcaaa ccccaatcat cgcattcaag gcggtggcta ttcgatgacg gagttatccc
     1861 tcatcaacaa aactcatatc gatggctatc tacaggtcac ggatgaagag gctatggaat
     1921 tcgcacgcag actcgcaaag gatgaaggaa tctttgcagg gttctcatcc ggcgcgaatg
     1981 tggctgccgc aatccaatta ctaaacacaa cttgcaaggg caaaacgatt gctgtattgc
     2041 tcagcgattc cggactcaaa tatttgagta ctgatttgtg gccataaact gtaaacacga
     2101 aagcactaac agaaatctta tggacgtttg atacactcac gattatgcac gaaaaaaaca
     2161 ttgaagaaag acaaaacttt caacacgaac agtacacaat gtttatcccg aactcaatgg
     2221 tggattcatg aattttccgg aaggattttt cgatcttctg aacgatcaat ccaaagctct
     2281 cctttacctc gcaacgataa tgcctgatgg ctcgccccag gtcacacccg tctggttcga
     2341 caccgatggc gaacacatcc tcatcaacac aaatgaaggt cgtgtgaaag atcgcaatat
     2401 gaaggcccgt ccgcgagtgg cgatgacaat tcaagatccg aatgatccct atcgctatct
     2461 cggtgtacgc ggtgaagttg ttaattacac aacaacaggt gcagatgagc atatcaacaa
     2521 actttccctt aaatatgacg atgaaccctg gacctatcgt gaatcacaaa gacgaattat
     2581 cttcacaatc aagccattac actttgacga acacaactga cctgcaggac tttttacgga
     2641 ctcgccgttc ggtccgccgc ttcaaaccgg acccggtgcc catagccgtt attcagacga
     2701 tcctcacgac agcaacatac gccccttctg cgcatcatcg tcagccctgg cgattcgttg
     2761 tggtgactga tatcggcgtc aaggctcggc ttgcggatgc tatggcaatt gattttgaac
     2821 gcgacctggc tcgcgatcaa ctccctgtgg aaaaaattca agcacagatc aaacgctcaa
     2881 aagaacgaat tacttctgca cccttggcaa tccttttatg cctggatatg agtgaaatgg
     2941 attcttatca agatgagaaa cgcaacaaag ccgaatacat gatggcggtt caatctgtcg
     3001 caaccgcggg attgcagctg ttgctggctg ctcatgcgga aggcttgggc ggcgtttggg
     3061 cttgttggcc gctgtttgcg caagcaacga ttcaaagtac cttgaactta tccgaagcat
     3121 gggaaccgca gggaatgttt ttccttgggt attatgaaac tgcgcccgaa gtaaggataa
     3181 gaaaaccctt gcaagacatc gtggtctttc gatgaaaata gttgccctcg ccggcggtgt
     3241 cggtggtgcg aaactggccc acggtcttgc ccaaattctc aaaccagaag aattaacggt
     3301 tatcgtcaat acaggcgatg attttgaaca ttatggtctt tatatttgcc ccgatctgga
     3361 tacggtttgt tatacgttag ccggaattgc aaatcccgaa acgggctggg gacgcgcggg
     3421 agaatcgtgg aatgccatgg agaacttgtc caagctcggt ggactcgatt ggttcaggct
     3481 tggcgacaaa gacttaggaa ctcaccttga aagaacaaga cgactgaaag aaggcttttc
     3541 tctcagccag atcaccaggg acttttgtaa ggcgtgggga atccaatttg ctgtgctgcc
     3601 gatgtccgac cagcccgtgc ggacctacgt cgaaacggac gagggtgagc tggcgtttca
     3661 ggaatatttt gtccatcgcc gatgcgaacc gcgcgtcaag ggcttctggt ttgacggcgt
     3721 ggaaagagcg gaacctgccc gcggggcact tgaagcgatc caggctgcag acgcaatcgt
     3781 catctgcccc tccaatccat gggtgagcat tgatccaatt ttgaaagtgc tttccctcac
     3841 cccagcccct ctcccactag gagaggagcg aagcagtgaa ggaaaaaggg tgtacgccgt
     3901 ctcccctatc gtaggcggac aagccatcaa gggtcccgct gcaaaaatgt acacagagtt
     3961 gggaattgaa ccttccgcct tcgcagtcgc aaatcattat cgtgggctga taaccgattt
     4021 tgtgctcgat caaatagacg agcaactgaa tgaaagtgtt aagggtttaa gtatgcagac
     4081 tcatgtaact aacacattaa tgaaaagtca tgaagataga aaaagattgg caacggactt
     4141 gctgaaattc atcggaagtg acttatgacg ctttgggcca ttgtacctgt gaagccactc
     4201 aggcgaggca aatctcgtct gtccggaatc ttgacagagg acgagcgggc ggatctgaat
     4261 cgcaagctct tgcaacatac actggaaacg ctgaacgagt taaaagaaat agaggaagtt
     4321 ctggtggtca gccgtgaccc ccaggtttta accattgcac gcaattacgg cgcgcgcacc
     4381 gttcgagagg atggtcagcc agatttgaac accgcgcttc gacgagcgac cgtcattgcg
     4441 gaagtctatg cgacgcgagg agtgttggtt cttcctgcag atttgccttt gatctcgcgg
     4501 gaggatgcgc gtgcattggt agaacgtgcc gtcgatccac ctgtggttgt gattgccccc
     4561 gaccggcacg aaaagggtac gaacgccctg ctcatctcgc cggctggtct gatcgagtat
     4621 gattttggcg agaactcttt tcaacgtcat tgtaatctgg taaaaaaggc aggtgtacgg
     4681 ctggaagtcg tcaacctgcc ctcacttgga ttggatctgg attcaccgga agattttgaa
     4741 atcataaaaa acctggaaag cacaatctca atatagatca tcaaggagaa tgccatgtct
     4801 cagcgtgttg cactatacct gcaagactcg cacgatttgc gcgatggact ggagtatgcg
     4861 agatatgctg aagagaaagg ctttgaagcc gtgtggcagg cagaatcgcg gctggtgcgc
     4921 gacgccattg tccctatggc agggtatgca gcggtgacaa atagaatcaa ggttgggtca
     4981 ggcgtcatca ataattggac ccgcaatatc ggattgcttg ctgcaacgtt tctcaccctc
     5041 gatgatcttg cgccaaaccg cgtgatctgc ggcattggtg cctggtggga tccattggca
     5101 aagaacgttg gcatcgaacg aaaaaaaccg cttttggcaa tgcgggaaac gattgaggtg
     5161 ttgaggcgtt tattgaatat ggagcgtgtc accttccatg gagaatttca tcacgtggac
     5221 gggatcgaac tcgacgttgt tcatgggcgc cgtgagccgc gcaatattcc catcatgatc
     5281 ggtgccaccg gcgacctcat gatggaattg acgggcgaaa tcgcagatgg cgcggtccta
     5341 aattattgtg tgccccctga ttacaatgac aacgccctgg aattactcga aaaaggattg
     5401 aagaaatcag gtcgcaagct ggaagatttt gatcgcccgc aattgatcgt ctgttcggtt
     5461 gatgaggatc acgacaaggc aatcgagtac accaaagaat tgctctgtca gtatctggcg
     5521 cagcaacccc atattgcaaa agcttcacgt gtttcacaga gcgtgatcaa cgaaatacaa
     5581 tccatcttag ggtggcccgc tacgaaggaa caaatcaatc aagccaagca tctcgtaccc
     5641 gatgagctgg ttcacaatat tactgcatcc gggacgccca ctgaagcgaa agccaaagtg
     5701 gaggaataca agaaacgcgg ctgtacctgc ccgatccttt acccggtcgg tggaaatttc
     5761 aaactactga tcgatacctt cgcacaggct tagagcttat tttgcaaata atcaagcaac
     5821 atcagcagcg cagcatccgc cgcggctgac ttattttgta ctcgatcccc tgagaattca
     5881 aataccctgg accattctcc atcatgcgcg gctaagccga tccacgtagt gcctactggt
     5941 ttctcggctg taccaccgcc aggacctgca attccactca ccgaaaccgc aatatccgca
     6001 ttcatcaact tgcgcgcgcc gcgcgccatc tcaatcactg tatcacggct caccgcgccg
     6061 gtggtattta gtgtgtccca ggagacacca aggatattta cttttgcctc attggagtaa
     6121 gagacaacgc ctcctaaaaa atattccgac gaaccagaaa catcagtgac gcggcttccg
     6181 accaacccac ctgtgcagga ttcagcgagg gccaatttcc agccccgttc aagcaacatc
     6241 gatccgattt gagtctccag cgtgtttgtc acagtaacct cagcgaattt gttatggaga
     6301 ttatacttgg ctcgattcat tctcggttat aatttcctct tatggcagac tgggccggaa
     6361 aaacgctcgg aaaggttcac attgatagcc ttgtagcccg tgggggcatg gctgaagttt
     6421 atacaggcac acatgagtct ttcgggcaag tcgccgtcaa agtgatgagg ggcttgctgg
     6481 aacgagattc agatcaactt gcccgcttcc agcgtgaagc ggaagtcgtt gaagagctgc
     6541 gtcatcccaa cattgttcaa atgtttgatt acacggtggt ggatgaaaca ccgtgcctgg
     6601 tcatggaata tatccctggc ccttcgctcc acgtttactt aaaacatctg cacgacaata
     6661 aacaacggct tcccattgga attgtggggc atattctgac ctcgatggca agtgctttgg
     6721 actatgctca ttccaaggga atggtccacc gcgatataaa acctgcaaac gtccttttac
     6781 gatccagatc tgagcccgtt gaattaaaca aacctcttcc tgcggatgtg gaacccgttc
     6841 ttaccgactt cggtctggtt cgcctgctcg actccacgat gcatacgaca gcaggatccg
     6901 tcagtggcac accaacatac atgagccccg aacaggcccg cggcgaaaag gtggataagc
     6961 gcacggatat ttactcattt ggcatcatgc tctatgagat gctggctgga gaggttcctt
     7021 tccagtctga tactacattt gggatgttga tgaaacatat caatgatcca ccggcgccca
     7081 tcaaagggat ttcaaaagac ctgcaggcgt tgctggaccg ggcgctcgcg aaagacccat
     7141 ctctacgcta cgagacggcg ggcgacctcg caaatgagtt catggcgctg ttcaatgggc
     7201 agaccatttc tccaggcacc ttgcacattg cagagatggc gcgcagggcc gcagaggcca
     7261 gcaagagggg ccaaacctcc gatggagtcc gacagggatg gtcaccgttg accattggtc
     7321 tcggcattgt cgcggttctg gggcttgcgt tcattatttc aaccttcttt agtccccggc
     7381 agaacacttt aactgttacg ccaacgccga ctagagatcc taacttcgat gtgggtcggt
     7441 tgcgcttcgg cgacgcaggc gacatcatgg acgaattagt catctcattg acaaatggcg
     7501 tgcagccacc tgcagacgga actcatttcg aagggtggct aatgaatgat gatggcaccg
     7561 taacacgtcc ccttgggccc gtaattttca atgatgcggg tgtaggtcga ttggagttca
     7621 gaaatctggg cagccaaaat attctcgcca ccttcaatca actcattatt tcagcggaag
     7681 tgggcaatat cgaagatgga gctgttgtaa ctcagccatc aacagatatt cggtattctt
     7741 caatatttcc acctgaggca ttgataccaa ttcgcaggat actggtatct gacccggata
     7801 caccggatga ggaagcgttg attcaggggc tttggtttta tggtgcacag tatattaata
     7861 ccagtataaa tggagatagt gaaaacgatc cggaatatat ctccctggtc gccgcgcttg
     7921 acgctgaaga tgaacctacg tttcgcaagc gcactgagga aatcatcaat caaatcgttg
     7981 gcgatgcaag tgaacaattt ttggactatg acgaggatgg cactgtagaa tattatttag
     8041 gcgatgccta tggcagttta cctgccggag atcgccctgg ttatattcag gctactgaaa
     8101 tgaacgcaca ggccgccgct gatgctgctg attcaaccgg aaatattcgt ttgtttagta
     8161 ataatactca aatatgtatg gagaacatga acacatggac caacgaactg ctccaacttg
     8221 ccttgcagtt ggttgatatg ccttttggtc cagagatgag gtcaacggtt gatcgaatgg
     8281 ctgaactcgg aaaatggctt tacgatggca atgacgtgaa taatgatagc caaatcgatc
     8341 cgatcccggg tgagtgcgga ataacgatcg cctacgaaga ggcttattcg atggcagata
     8401 tgctgattta tccgggccca gatcggctgc aacccactgg aaaataaaat ctcccgcaag
     8461 cgggagattt tattttcggg cgtgctattg aactaagtat tagggggtaa tgctttgatt
     8521 gatctggttg aagatgtcct gcatttgtgg cccaagaatg ctaaacagac caccgatagc
     8581 caccaaaaca cagcagaaaa tcacaacacc tgggagaagc agagaagcgg ccgccttgcc
     8641 ccagtcaaat tggttgacgg cttttacagc catcacttcc agataaagca cataaagagc
     8701 cgccagtaga cttacaagac caaaacaaaa acccacaaac gggatggccg atagcaacgt
     8761 aaacaacgcg ctaacaagtg taaacggaac agtgatggca gccagcacat aaacgagctt
     8821 ctcaaatgac ccgcgaccac caaacaatcc ggctatccac tgcacaatac cggtaaacga
     8881 tgcaaaaagt acgacggaaa tggctgcagc tacaggagcg ccacagagta caccaatcag
     8941 gctggtgctc atcgggcttt cgaagccgga accctgcatc attcgattca tcaagacgcc
     9001 ctgcaacaga aaaacgataa atgactgaat tagggagccg acaaaaaccc atagtaaagc
     9061 cgtaccggtt tttgcttgag gcgaattagc gatttgaaca taagtctctt cactgggttt
     9121 tgtgagggca ttgatccagg tctgaaacat gggttgcggc ctgtcgggta acattggtgc
     9181 attcggctgt tcgttcataa gttcctccaa gaattaaaga aaaatttagc ttattatggc
     9241 acacttaatg accaattgca actctgcaac acacagccta gatgagttct gcgtgctgct
     9301 gcacagaaat gcaggataag tccggatcag taaactcaag acaagcggta aaattattca
     9361 cgatggagat ctctgagaaa cttcaaggca ttcttgccac cttaccggcc aagcctggct
     9421 gctatctgat gaaaaataat gaggggaaga tcatctacgc gggtaaggca atcagcctga
     9481 aaaaccgcgt ccggtcttat tttcacgcgg atgccagcca cgacacgaaa acgcgtcgtc
     9541 ttgtgcgaga gattgtagat atcgaatgga ttgtggtggg gtcggagctc gaggcgctca
     9601 tcctggagat gaacctcatc aagaagcatc ggccgaaata caacgtccgg cttaaggatg
     9661 ataaacgtta tccgtacatc aagatccatt gggcagaccc gttcccgaaa gtcactgtga
     9721 cgcggcaaat ggtcgaagat gggtcacggt attttggacc ctatacctct gcctgggctg
     9781 tataccagac tctggatgtg cttcgacgca tcttccccta tttgacctgc gatcgcaaca
     9841 tcaccgggct ggatcaacgc gcctgcctgt actacgatat caaactctgc actgctccat
     9901 gtatcggcgc ggtcaatcag gctcaatatc gtcaaactat ctctgacctg atggatttcc
     9961 tcagcggaca tagcgacgat atcgtcgccc gccttcaagc ggatatggag aaggcctctg
    10021 aagagatgcg cttcgaaagg gccgccgcgc tgcgagatca gctcaaagcc attcaagcca
    10081 tcattgagcg gcaaaaaatt gtttttgcaa gcgattatgt ggattcagat gtactggcca
    10141 tggcacgcgc aaatggcgaa gcctgtgtgc aggtcttttt catccgcggc ggaaagttga
    10201 tcggacgcga atactttatc ctggaaggta ccgaagacga agcagatagt gagatcatgg
    10261 agcagttcgt gacgcagttt tatacagaag ctgccacggt gcctccacag gtgatgctgc
    10321 ctgaagaaat cgaagaagct cgcattatca gccagtggct acgctctcgg cgcggtggtg
    10381 aaaaagtgga attctttgtt ccaaaaga
//
LOCUS       DASTJP010000507         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_186107, whole genome shotgun sequence.
ACCESSION   DASTJP010000507 DASTJP010000000
VERSION     DASTJP010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186107"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFQ23_14885"
     CDS             complement(<1..579)
                     /locus_tag="VFQ23_14885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9907934.1"
                     /translation="MPRVLYTAFDIVPGPKGASTHILHNIRGLVNGQFDVHLLTPNDG
                     VLPMEDMVEGAHVTRITQDLTQNFLARAAHFGQAVLTHLTLNAGYDVVHYRNIWDGLA
                     VAQNKKHFGYKTLFEVNGLPSIELKYHYPGIDSGLLSKIKEQEIATLHLSDVIICPSN
                     VTRDYIASLGLDRKRVTVIPNGVSPSDFAPSPL"
     gene            705..1778
                     /locus_tag="VFQ23_14890"
     CDS             705..1778
                     /locus_tag="VFQ23_14890"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HET9907935.1"
                     /translation="MANRGYFIITDISGYTEYLTESELDHAHEILQSLFDAQLKQIKF
                     PLKISGFRGDAIFMYTPEACFVNPQSFIETLENLYFEFLESAQQMQFNTTCPCNACRN
                     IHKLDLKMCIHYGEYIIQKLGDREELLGADVIVPHRMLKNHVLEQTGIRSYALFSKAA
                     AQSLELHNLCEPLVPHQESYEHLGEVRMLVHDLRAAWESEQARKKIRVDPNEAWFTLE
                     VDVPFPPSLVWDAITTPALEGPLLGLDYVKRVDDLGGRTRPEAKFHCAHGSGDFFNTV
                     VDWKPFDYFTVYQHVAGLEYFRTISLEYDGSKTKFVMLVSKPEQDAPEGFREFLEMAA
                     RQGYERLPLALQSQMDSSKITTQ"
     gene            1788..2735
                     /locus_tag="VFQ23_14895"
     CDS             1788..2735
                     /locus_tag="VFQ23_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907936.1"
                     /translation="MESFLCVGGLLLVGVFILFSYFLNTARKVAPPTNVAGKKELLPV
                     YISLADKPHSIIQGMEKFRAEAQKTETTGDKWRWVPMLIFFAGIGLMLVDVVLWLLGY
                     SSFIFVLGGISLWIAAIILARSLRKSDLHDFSPRYNGAKEILHTLRDDLRPNSSFLGH
                     LDLTGALVSTKVARTAQDTQKRTTEYYRDEWLALKAKLYDGNILRVSAIQKSKKRKSY
                     WKTSRISGKSKLKPEKYKGSQHDLKVRIVVNPELYKIESSSATFKQGLNIGKFTISQL
                     STEGGMINFVANSPFDEVEHENILTLLQSAYSLLKRKAA"
     gene            2736..>3530
                     /locus_tag="VFQ23_14900"
     CDS             2736..>3530
                     /locus_tag="VFQ23_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907937.1"
                     /translation="MSRRIIPFAVALVLICLVMAVSAVFSFSGAITAEKFGSNVAWSQ
                     PYSTAESMNIINVLGDDQDDLFIQNTSNVSVYDSNGSVVFTKDYQFPKTTLGDINADG
                     MEDIVVFYLGDLGYSVDTIINGETSTLAQTLTIGQPARVAILRFASGPQIVLGDTSGS
                     LLALSMEGAPLWESKLGSSEVRGMDDARIGGQIHLAAATNDGSLGVYDSQGVQVWSSS
                     QEQLRRMRAFDLNGDGNSEVITGGEYGAFFIYSAADGSTVHSASLGQ"
BASE COUNT          935 a          843 c          893 g          859 t
ORIGIN      
        1 caagggcgaa ggtgcaaaat ccgaagggct gactccgttg gggatcacgg tcacccgctt
       61 gcggtccaga ccaagcgacg cgatatagtc ccgcgtcaca ttcgaagggc agataatcac
      121 atcgcttaag tgcaacgtgg cgatttcctg ttcttttatt ttagagagta agcctgagtc
      181 gattccggga tagtgatatt tgagttcaat cgatggcagc ccattcactt caaacagggt
      241 tttatacccg aagtgtttct tattctgggc aactgcgagt ccatcccaaa tgttacgata
      301 atgaactaca tcgtagcccg cattaagagt aagatgtgtg agaacggctt gtccgaaatg
      361 agcggcgcgc gccagaaaat tttgagtgag gtcttgtgta atacgcgtga catgcgcccc
      421 ttcaaccatg tcttccatgg gcagcacacc gtcatttggt gtcagcaggt gcacgtcaaa
      481 ctgaccattg acgagcccgc gaatgttatg caaaatatga gtcgatgccc ccttgggtcc
      541 cggaactatg tcgaaggcag tgtagagcac gcgaggcatt gtagagatta taagctagca
      601 aatgtcattg cgaggagcgt atgctgcgac gaagcaatct tctctttagt ttgagattgc
      661 ttcgggcgga caagcaccac cctcgcaatg acatgaggaa acttatggca aacagaggct
      721 acttcatcat cacagacatc tcgggataca ccgaatacct gactgaatca gaactcgatc
      781 acgcccacga gattttgcaa agcctatttg acgcacaatt aaaacagatc aaatttccgc
      841 ttaagatctc aggtttccgc ggcgacgcga tcttcatgta tacgccggaa gcctgtttcg
      901 tcaatccgca aagtttcatt gaaacacttg aaaatctata ttttgaattc ctggaatctg
      961 cgcagcaaat gcagttcaac acgacctgcc cttgcaacgc ctgccgaaat attcataaac
     1021 tcgatctgaa aatgtgcatt cattatggcg agtacatcat tcaaaaactg ggagaccgtg
     1081 aagaattgct tggtgcagat gtcattgtgc cacatcgcat gctgaagaat catgttctcg
     1141 aacaaacggg aattaggtcg tatgctttat tctctaaagc tgccgcacag tcattggagc
     1201 tacacaatct gtgcgagcca ctagttcctc atcaagagag ctatgaacac ctcggcgaag
     1261 tgagaatgct cgttcacgac ctgcgagcgg cgtgggaaag cgaacaggcg cgcaagaaaa
     1321 ttcgggttga cccaaacgaa gcctggttca cgcttgaagt tgatgttcct tttccgccgt
     1381 ctttagtttg ggatgcaatc actacaccgg ctctcgaagg tcccttgctg ggattagact
     1441 atgtaaagcg tgtggacgac ctcggcggac gtacgcgccc tgaagcaaaa ttccattgtg
     1501 cccacggcag tggcgatttt ttcaacacgg tcgtagattg gaaacccttt gactacttta
     1561 ctgtatatca acatgtggcc ggcttggaat attttcgcac catcagtttg gagtacgacg
     1621 gatcaaaaac gaaattcgtg atgctcgtca gcaaacctga gcaggatgcg cccgaaggct
     1681 ttcgcgagtt tcttgagatg gcagcacggc aggggtacga acgtctcccg ctggcgctac
     1741 aatcgcaaat ggatagcagc aaaatcacaa cacaataagg caactccatg gaatcatttc
     1801 tttgtgtggg cgggcttctt ctcgttggcg ttttcatatt attttcttat ttcctgaata
     1861 cggcgcggaa ggtcgcaccg ccaacaaacg tcgctggcaa aaaagaacta ttgcccgttt
     1921 atatttcact ggcagacaaa cctcattcca tcattcaggg aatggaaaaa ttcagggcgg
     1981 aagcacaaaa gacggagaca actggcgaca agtggcgctg ggtgcccatg ctcatttttt
     2041 tcgcgggtat cgggttgatg ctggtggacg ttgtattatg gttgctgggt tactcatcct
     2101 ttatctttgt gctgggtgga atatcgttgt ggattgcagc gatcattctg gcacgcagcc
     2161 tgcgaaaaag cgaccttcat gatttttctc cgcgctacaa cggagcaaaa gaaatcctgc
     2221 atacccttcg cgatgacctg agaccaaact ccagcttcct cggacatctc gacctgacgg
     2281 gcgccctcgt atcgacaaaa gttgcgcgaa cagcgcagga tacgcagaaa cgcacaaccg
     2341 aatactacag agatgaatgg ctggcgctga aagccaaact ctatgatggg aacattttgc
     2401 gggtgtctgc gattcaaaaa tcaaagaaac gaaagtccta ctggaaaacc agtcggatca
     2461 gtggcaagtc caaactcaag cccgaaaaat ataaaggcag ccagcatgat ctgaaagtgc
     2521 gtatcgtcgt caaccctgaa ctctataaaa tagaatcatc aagcgccacc ttcaaacaag
     2581 ggcttaacat cggtaaattt acgataagcc agttaagcac agagggcggc atgatcaatt
     2641 tcgtcgcgaa ttccccgttc gatgaagtgg aacacgaaaa catcctgacc cttcttcaat
     2701 cggcatactc cctcttgaaa agaaaggcgg cataaatgag tcgcagaatt attccatttg
     2761 ctgttgcgct ggtcttgatt tgtctcgtca tggctgtatc ggctgtgttt tcttttagcg
     2821 gagcaataac agcggaaaaa tttggaagta acgtagcctg gtcacagccg tattcaacgg
     2881 ccgagagcat gaatatcatt aatgtgctgg gcgatgatca ggatgatctg tttattcaaa
     2941 acaccagcaa tgtgagcgtg tatgacagca atggcagcgt ggtctttacg aaggattatc
     3001 aatttccaaa aacaacactg ggtgacatca atgcagatgg gatggaggat attgttgtct
     3061 tttatcttgg tgacctgggc tattccgtgg atacgatcat caacggcgag acgtcaaccc
     3121 tcgcgcagac tttaacaatc ggtcagcctg cccgagttgc catcctccgt ttcgcctctg
     3181 gacctcaaat cgtgttgggt gatacgagcg gcagtctgct tgctttgagt atggaaggtg
     3241 ctcctttgtg ggaatcgaaa ctcggcagca gtgaagtacg cggcatggat gacgccagaa
     3301 tcggtgggca gatccactta gccgccgcaa cgaatgatgg gagccttgga gtctacgatt
     3361 cgcagggtgt gcaggtctgg tcttccagcc aggaacaact ccgccgcatg cgcgccttcg
     3421 acttgaacgg ggatggcaac agcgaagtga tcacaggcgg ggagtatggc gccttcttta
     3481 tttacagcgc cgccgatggg agcacggttc atagtgcatc actggggcag
//
LOCUS       DASTJP010000508         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_229283, whole genome shotgun sequence.
ACCESSION   DASTJP010000508 DASTJP010000000
VERSION     DASTJP010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229283"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFQ23_14905"
     CDS             complement(<1..876)
                     /locus_tag="VFQ23_14905"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HET9907938.1"
                     /translation="MSDETLPPPVWVANQDALQQMAAKLSAHPRIAVDTESNSLHAYR
                     ERVCLVQFSIPNVDFVVDPFAIQDLSSLAPIFASPDIEKIFHAAEYDLICLRRDFGFA
                     FANLFDTMQAARILGYSFVGLDNILLEKFDIKIDKRHQKANWGARPLTWQQIDYARQD
                     THFLFRLRDLLENQLREMGRWEIARDDFALACHVEIPKERGTGSSWRRFAGRKDVSTR
                     ELTILYELCQARDQIAEKLNRPTFKVIADEILLAMARKVPSTNEELIEIGLSPKQVRL
                     WGNLILSAIARGVDAP"
     gene            complement(938..1369)
                     /locus_tag="VFQ23_14910"
     CDS             complement(938..1369)
                     /locus_tag="VFQ23_14910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907939.1"
                     /translation="MELQPATIRDLSAVRRLEKICFEKDAWPVLDLIAALTWPNVIRI
                     KAVDDGTMVGFVAGESRSSNREFWIATLAVHPNYQRRGIGRQLLRACEQQAKLHQVKL
                     TVRISNQAAISLYEKEGYRTVDIWKGYYVDGEDGLVMGKMV"
     gene            complement(1453..>2169)
                     /locus_tag="VFQ23_14915"
     CDS             complement(1453..>2169)
                     /locus_tag="VFQ23_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9907940.1"
                     /translation="DRAVKVDLREQVREVPHQTSITKDNAPISIDFLWYYKVLDPAES
                     VLQVGNFESAAAGIAATTLRAVIGGIPLDDVLSEREHINNMLRTRLDEVTERWGVKVT
                     NVEIREIIPPRDVQDAMNRQMSAERIRRAVVTESTGTREAAVNVAEGEKQSNILRAEG
                     QKQSAILAAEGEKAAQLLRAEGFAQALEQIFNAAKSVDQKTMTLQYFETLKSIGASPS
                     TKYIFPMEFTSMLDNFLGKK"
BASE COUNT          547 a          568 c          487 g          567 t
ORIGIN      
        1 tggcgcatcc actccccgcg cgattgctga caagattaaa tttccccaca aacgaacctg
       61 cttcgggctt aatccgattt caattaactc ttcattggtc gacggaactt tacgcgccat
      121 tgcaagcaga atttcatctg caatcacttt gaaagttggg cggttcagtt tctcagcgat
      181 ctggtcgcgg gcctggcaca attcatacaa tatggtcaac tctcgagtag agacatcttt
      241 gcgccctgca aaccgccgcc atgatgagcc tgtgccgcgc tctttgggta tttctacgtg
      301 acaggcgaga gcaaaatcat ctcgtgcaat ttcccagcgt cccatctcac gcagctgatt
      361 ttccaataga tcacgcagac gaaaaagaaa atgagtgtcc tgccgggcat agtcgatttg
      421 ctgccaggta agcggccgcg caccccaatt ggctttttgg tgccgtttat cgatttttat
      481 gtcgaacttt tccagcaaaa tattatccaa tccgacaaaa gaatacccaa gaatccgtgc
      541 cgcctgcatc gtatcgaaca aattcgcaaa agcgaatcca aaatctcgtc gaaggcagat
      601 caggtcgtat tccgcggcat gaaatatttt ttcaatatca gggctggcaa aaattggagc
      661 gagggaactt aggtcctgaa tggcaaaggg gtcaacaaca aagtcgacgt ttggaatcga
      721 aaattgaaca aggcaaactc tttcacgata ggcgtgcaaa ctattggatt cggtatcaac
      781 cgcgatgcgt ggatgtgcag ataacttcgc agccatttgc tgcagagcat cttgattagc
      841 cacccatacc ggtggcggca atgtttcatc gctcatcaat tgtcattata tcccaccacc
      901 agcgttattt ctcatagcaa tagtctagca aatgacccta taccatcttt cccatgacta
      961 atccatcttc cccgtctaca tagtatccct tccaaatatc cacggtgcga tagccttcct
     1021 tctcgtaaag agaaatcgcc gcctggttgg aaattcgcac cgttagcttc acctgatgaa
     1081 gttttgcctg ttgttcacaa gcacgcagca attgacgccc aataccccga cgctggtagt
     1141 tcggatgaac agcaagtgtt gcgatccaga attcccgatt ggaagaacga gactcacctg
     1201 ccacgaatcc aaccattgtg ccgtcatcga ccgccttaat ccggatcacg ttaggccagg
     1261 tcaaagcggc gatgagatcc aaaactggcc aggcatcctt ctcgaaacaa atcttttcca
     1321 gtctgcgcac tgcgctcaga tcgcgaattg ttgcaggttg tagttccatt aggaagaatt
     1381 ctatcaataa aaaagtagaa agtggaaggt agtctctcca ctttctactg atctcacttt
     1441 atattgaatg gcttattttt tgcccaggaa attatccaac atgcttgtaa attccatcgg
     1501 gaagatatat ttcgtggatg ggctggcgcc aattgacttt agtgtttcga aatattgaag
     1561 tgtcatcgtc ttctggtcaa cggattttgc agcattgaag atctgctcaa gggcttgcgc
     1621 aaaaccttct gcccgcagca gttgagcagc tttttcacct tctgccgcaa ggatggcaga
     1681 ttgtttctga ccttctgctc gcaaaatatt ggattgcttc tcaccttccg ccacatttac
     1741 ggcagcttcg cgcgtacccg tggattcggt aaccacagca cggcggatgc gctcagcgga
     1801 catttgtcgg ttcatggcat cctgcacatc gcgcggcggg atgatctcgc gaatttcgac
     1861 gttggtcacc ttgactcccc atcgttccgt aacttcatcc aaacgcgtac gaagcatgtt
     1921 gttgatatgc tcgcgctcag aaagtacatc gtccagtggg ataccgccga tgaccgcgcg
     1981 taaagtggtc gccgcgatac cggcagccgc cgactcaaaa ttaccaacct gcaacactga
     2041 ttcagccggg tctaaaactt tgtagtacca aaggaagtca atcgagatcg gcgcattatc
     2101 ttttgtgatc gaggtctgat gtggcacctc gcgtacttgt tcgcgcaagt ctacttttac
     2161 agcgcggtc
//
LOCUS       DASTJP010000509         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1086313, whole genome shotgun sequence.
ACCESSION   DASTJP010000509 DASTJP010000000
VERSION     DASTJP010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086313"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VFQ23_14920"
     CDS             complement(<1..1305)
                     /locus_tag="VFQ23_14920"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HET9907941.1"
                     /translation="MPNNIPVSLTSLVGREQEIETLCQLLHRPDVRLVTITGLGGVGK
                     TSLALQVAHELREAFDDGVVFISLAPIRDPTLIIPTIARLLDVTESPNRLMLDSLKDF
                     LRDRQMLLLLDNFEQIVAAAPLLTELLEACPELRMLVTSREVLRLRGEHEFPLLPLAL
                     PDQPSLEIMMQYPSVALFVQRAQASQPDFKLTPENTALVTEICARLDGLPLAIELAAA
                     RIKLLPPQAMLRQLQESPLQMLTGGARDLPARQQTLRGTVQWSYDLLNADEQRLFRWL
                     SVFVGGCTLEAAAKAMGQQATLDNLDSLVNKSLLRQHETDHSPRLSILETIREFGFEQ
                     LNHTHELESARRAHATYYLTFAEDAERELTGADQKTWLQRLEREQDNMRAALHWAIEH
                     SEVEFAQRMAGALQPFWFKRGHWSEGRRWLEDSLVIESNAALN"
     gene            complement(1355..1498)
                     /locus_tag="VFQ23_14925"
     CDS             complement(1355..1498)
                     /locus_tag="VFQ23_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907942.1"
                     /translation="MERFELNTVIDRPIDEAFAVPSNLENDLAVDIDSIYDVSPFDSV
                     KYK"
     gene            complement(1514..1993)
                     /locus_tag="VFQ23_14930"
     CDS             complement(1514..1993)
                     /locus_tag="VFQ23_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907943.1"
                     /translation="MYNWLVFLHVFFAFLFMLTHGAHAAAMLKFRGEPDPERSLTFFN
                     NVPDSKYVRYLTVAMGLFGFAPAFMTVWWRQWWIWLSVLVFLIVSWVMVQYGAGYYGM
                     IFNAAQRLIEARKTNVDLSAAQKEFDEVRNAPNALIVSVTGIVGLAILLWLMRFKPF"
     gene            complement(2127..2618)
                     /locus_tag="VFQ23_14935"
     CDS             complement(2127..2618)
                     /locus_tag="VFQ23_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907944.1"
                     /translation="MSTIATTSPTSRNSLLRLTLIGGFIAGLLHLVVQQGIVFGLILK
                     TPIVSALQFAASGIMGEAAFAGGLATALFGLVLDFIMITIMAGVFVFSVDRIPLLRRN
                     VIVGSILYGFGIFIVMNFIVLPLSAAPALPAPPLWLLIELVLQHNLLIGLPLGILVQR
                     NTR"
     gene            complement(2668..3171)
                     /locus_tag="VFQ23_14940"
     CDS             complement(2668..3171)
                     /locus_tag="VFQ23_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907945.1"
                     /translation="MSTIANKPPTSSNSLLRLSVVTGLVISTADMIIYNWLVVSILFG
                     IPLITFFQYIASGALGESAFAGGIATAFIGVLFYFLISIVVAGVFILGVDRIPLLRRH
                     IIPGSLLYGFGVFIVMNMIVTPLSATPPIPAPTTQELILIIVDHLLFYGLLLGILVRR
                     NINKKES"
     gene            complement(3285..3596)
                     /locus_tag="VFQ23_14945"
     CDS             complement(3285..3596)
                     /locus_tag="VFQ23_14945"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9907946.1"
                     /translation="MSMENEVSIKVIVELHAKPGRRAELKNLLASVVAKYGAVQPGFL
                     GSLQYEVIDNPDILVEIADWASAEVRATAMQQAMNAGAYAPLEELLAAPFRATVIRQL
                     L"
BASE COUNT         1002 a          965 c          993 g          928 t
ORIGIN      
        1 attcaaagca gcgtttgatt caattaccaa tgaatcttcc aaccagcgac ggccctcgct
       61 ccaatgccca cgcttaaacc agaacggttg cagcgcgcct gccatgcgct gggcgaattc
      121 aacctcgcta tgctcaatcg cccaatgcag cgccgcgcgc atattgtcct gctcgcgttc
      181 caatcgttgg agccaggtct tttgatccgc gcccgtcaac tctcgttcgg catcttccgc
      241 aaatgttaga taataggtgg catgagcgcg ccgcgcggat tccagttcgt gtgtatggtt
      301 caattgctca aagccaaact cccggatggt ctccagaatg ctcaagcgcg gtgaatggtc
      361 tgtctcgtgt tgcctcaata gacttttatt tacgagtgaa tcgagattat ccaacgttgc
      421 ctgctgaccc attgcttttg ccgcagcctc aagtgtgcat ccacccacaa aaaccgacaa
      481 ccagcggaag aggcgctgct catccgcatt caataggtca taactccatt gaactgtgcc
      541 gcgcaacgtt tgctggcgcg caggcaggtc acgtgcgccg cccgtgagca tttgtaaagg
      601 tgactcttgc agctgcctaa gcattgcctg tggcggaagc agtttgatgc gcgccgcggc
      661 aagttcaatg gcgagaggca gcccgtcgag gcgggcgcag atctcggtca caagtgcggt
      721 gttctctggc gtcagcttga aatcgggctg gctggcctgg gcgcgttgga cgaataacgc
      781 aacgcttgga tattgcatca taatttcaag cgaaggctga tcgggcaacg caagcggcaa
      841 caacggaaac tcgtgttcgc cgcgcaagcg caagacttca cggctggtga ccaacatccg
      901 caactcgggg caagcttcca ataattctgt caataacggc gcggcggcaa cgatctgctc
      961 aaagttatcg agcaataaca acatctgccg gtcacgcagg aagtctttca ggctgtccag
     1021 catcaaacga ttgggcgatt cggtaacgtc aagcagacgc gcaatcgttg gaataattaa
     1081 ggtagggtca cggatcggcg cgagcgaaat aaaaacgacg ccgtcatcga aggcttcgcg
     1141 taattcgtgg gcaacctgta gtgcgaggct cgtcttaccg acgccgccga gaccagtgat
     1201 cgtgacgaga cggacatcgg gtcgatgcag gagttggcat agtgtctcta tctcctgttc
     1261 ccgcccaaca agtgaggtca gcgatacggg gatgttattt ggcattacag atattatatg
     1321 tggattgttt cgtacttgta gaatcatttg cttcttattt gtacttaact gaatcgaaag
     1381 gggatacgtc gtaaattgaa tctatgtcca ccgccagatc attctcaagg ttggacggaa
     1441 ccgcaaaggc ctcatcgatg ggacggtcga tcacagtgtt cagttcgaat ctttccattt
     1501 tcctccagac aacttaaaac ggtttgaacc tcatcagcca gaggaggatg gccagcccaa
     1561 cgatacctgt aaccgacacg atcagcgcat tcggcgcgtt acgcacctca tcaaattctt
     1621 tttgcgcggc ggacaagtcc acatttgttt ttctggcttc gatcaggcgc tgggcagcgt
     1681 taaagatcat gccatagtaa cctgccccat attggaccat gacccaggat acgatcagga
     1741 agaccagcac tgaaagccag atccaccatt gccgccacca tacagtcata aatgcaggcg
     1801 caaagccgaa gagccccatg gcgacagtga gataccggac gtacttgctg tcgggaacat
     1861 tattgaagaa agtcaggctt cgttcgggat cgggctcgcc gcggaatttc agcatcgcgg
     1921 cggcgtgggc tccgtgtgtg agcatgaaga gaaaggcaaa aaagacgtgc agaaatacga
     1981 gccagttgta catatcagac tccttaacca gcagaacgag tccaggtcat tgcgagcgca
     2041 cagagcgaag tgatctgtgt gcctactgca gcggactcaa gccggatact tcccttgacc
     2101 agtcctctca tcagggattc tcgggttcac cgcgtatttc gctgtacgag gattcccaac
     2161 ggcaacccga tgagcagatt gtgctgcaag acgagttcga tgagcagcca caatggtggc
     2221 gctggcagcg cgggcgcggc gctgagtggc aggacaataa agttcatcac gatgaaaatg
     2281 ccaaacccat ataggatcga gcctacaatg acattgcgac gcaggagcgg aattcggtca
     2341 acgctgaaaa caaaaacgcc agccataatc gtgatcatga tgaaatcaag gaccagccca
     2401 aacaaggcag tggcaagtcc gcctgcaaaa gcggcctcac ccattatgcc gctggctgca
     2461 aattgtaatg cgctaacaat tggagtctta agaatcagcc cgaaaacgat tccttgttgg
     2521 acgacaagat ggagaagacc tgcaataaac ccgccgatca aagttaagcg gaggagtgaa
     2581 ttgcgggagg tgggggaggt ggtagcgata gtagacatgt tattagttcc tttctttgtg
     2641 attaagagag ttaatgtttg ctgccgatca gctttctttt ttgtttatat ttcgacgcac
     2701 aagtattccc aatagcaacc cgtagaatag caggtggtct acgatgatca agattagctc
     2761 ttgcgttgtc ggagctggga ttggaggtgt cgcgctgaga ggcgtaacaa tcatgttcat
     2821 gacaatgaaa acaccaaatc catacaggag cgagccaggg atgatatggc gccgcaggag
     2881 cggaatgcgg tcaacaccca ggatgaaaac gccagcgact acaatgctaa tgaggaagta
     2941 gaatagcact ccgataaaag ctgtagcgat gccacccgca aaggcggatt cacccaatgc
     3001 gccactggct atatattgaa aaaatgtaat taatgggatc ccaaaaagga tacttacaac
     3061 aagccagtta taaattatca tatcggctgt actgataacc aatcctgtga ccacacttaa
     3121 gcggaggagt gaattgctgg aggtgggagg cttgttggcg atagtagaca taataaagat
     3181 tcctttcttt accgcactaa tttgtgaccg agaattgact gaaaattatg gtccatcttt
     3241 ttttctcctt atgatcgtca taaattattg gatgtttgaa cgaattacaa caattgccgg
     3301 atgaccgttg ccctgaacgg tgcagctaac agctcttcaa gcggtgcata agcaccagcg
     3361 ttcatggctt gttgcatggc ggttgcccgc acctccgctg aggcccaatc ggcgatctcg
     3421 accaggatgt cggggttatc gatcacttca tattgcaggc tgcccaggaa tccaggttga
     3481 accgctccgt atttcgccac cacgctcgcg agcagattct tgagctcagc ccgcctgccc
     3541 ggtttggcgt ggagttcaac gatcactttg attgatactt cattttccat tgacatacat
     3601 tactcctgcc gtatattgaa ttgatttgca gtgcaatcgg ttattctgca aacaatcagt
     3661 ggggacggat catggctgtt gtaacaatac gtgtttcctt tctgacgcga atcatgtaat
     3721 aattgtctat gctcgttgct ctttactctt tgatacttta gcgaaatgcc attaattcat
     3781 cattatttgg acacacattg tctaaattga ttttttcttt attgggtagt ttgcagatca
     3841 aacatcgcca attaggtttc attacgctcg ccaaccgcaa agcgattg
//
LOCUS       DASTJP010000510         4505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_489180, whole genome shotgun sequence.
ACCESSION   DASTJP010000510 DASTJP010000000
VERSION     DASTJP010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489180"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VFQ23_14950"
     CDS             complement(<1..1030)
                     /locus_tag="VFQ23_14950"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9907947.1"
                     /translation="MRQLVEHRWFALADLFLVMISAVVWMLIPQFGIGFSLIALLPWV
                     FRFFMRQSLIQWTLLDWMIIIFLLTAWVGYWTAYDKSAAGIKFWLIVNAVLLYYSLSS
                     QPNQNLSILSFLSFCFAFALSLYFCLTYDFSGIGGGIGTWWTTSRPRVDWPAIHYDDV
                     LGPILISAILAFYWLSSSIKKSSGALAAFMQLFLVLGIGIVALVFVLTLSRGIVLIAL
                     FAVGLSTLWKVSTYSMSNVRARALFPTLVLILLVLLILVAYLGPASEDPASAPSDYGT
                     NTRAELFERGSYLLVDYAITGAGLTSFPGLYSQYMLVIPHFYFPNSYNLFLDVAVEQG
                     VIAGLIFIL"
     gene            complement(1061..2725)
                     /locus_tag="VFQ23_14955"
     CDS             complement(1061..2725)
                     /locus_tag="VFQ23_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9907948.1"
                     /translation="MKSNEPASVNLGSRLTPKLHKANFPNADAIVRDGLLSAPRSADA
                     VDIMLVNPPTPDGELWIRTQHRVGRRTRENMVWPQVSLAQMAALLHPVYKVKVVDCNA
                     ERMGWAEFTKLLDQYQPKYYLTQLTAPTLENDMYGCFLAHARGAKTIAFGTHITPIPV
                     ETMRPFPSLDFALVGEPDLTIRDLLDHLENKIDQRSPELQLIFDKHDLMYKPSFNEDG
                     TVNMHGIKGIAWRKNGEIILNFPRPFVADLDDMPIPMHELLPLQKYRMPYIKGPFTFM
                     LTSRGCPAGCTYCIKHVSYQYATRLRSPKLILEEMWYLKKLGINNIHMYADLFTVNRD
                     QVIEMCKLMIEQKIDIHWTSNSRVDFVDEEMLMYMGKAGCRLISWGIESGNEAILKHA
                     RKGADPEKAERALRWAKQAGIMNWGYFIIGLPGETEDTIRQTIDFAKKLPLDIALFHV
                     AAPYPGTPFFFEVVKNKWFRPGTRWEHVDMDKGTVLDYPNMPAERLLYWQKRAFREWA
                     FRPGPAFTYLKMLLYDWSTMKTALNVGLQHLSWASSDEGLPIGGGG"
     gene            complement(2762..3958)
                     /locus_tag="VFQ23_14960"
     CDS             complement(2762..3958)
                     /locus_tag="VFQ23_14960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9907949.1"
                     /translation="MTLLSIVIPAYNEEKGIAEIAHRVLSVGPDLKNAGVDELELLIV
                     DDGSKDRTVEVASKIAGVNLICHPRNKGYGAALKTGFSQARGELIGFLDADGTYPPEY
                     FPQLCRAALNGGDLIIGSRMAGAESKMPLTRRIGNFFFAGLLTLLSRQKVTDSASGMR
                     VFKREILEQVYPLPDGLNLTPVMSTRALHEGINIGEVPIPYSERVGRSKLSVIRDGRI
                     FLQSMVWTVLSYNPVRILGMIGATGIGIAALVFLGFVIARLQGITALGPWGVAALFLA
                     LISSVAGINIFALGVTFNYLVSLFYRKPIRQGLFGKPIFKAPLDLQFGWMGVVAVVLG
                     IAIAVISLALGVQGWTIDRLWFYLLGSALLFLAGIQLSIYWILLRILEELSKREFLTK
                     QDMGLS"
     gene            complement(3979..>4505)
                     /locus_tag="VFQ23_14965"
     CDS             complement(3979..>4505)
                     /locus_tag="VFQ23_14965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9907950.1"
                     /translation="DHIRLGHGVYIDQGAYLHACPQGIDIGDGTIVMHGAVLHVYNFR
                     NLPHSGIRIGRDSLIGEYSVIRGQGGVQIGDRVYTSPFTQIIAVNHVFDDPNRPFVEQ
                     GITAQGIVIEDDVWLGSGAIVTDGVRIGKGAVIAAGAVVTKDVAPHTVVGGVPAKPIK
                     VIDGQSNQSGRQVF"
BASE COUNT         1300 a         1141 c         1025 g         1039 t
ORIGIN      
        1 gaaggataaa tattaagccg gcgattacac cttgttcaac ggctacatcc aggaatagat
       61 tgtaactgtt tggaaagtag aagtgcggaa ttaccaacat atattgggaa taaagaccag
      121 gaaaagaggt aagtccggcc cccgtaatgg catagtccac taataaatag gaacctcttt
      181 caaataattc tgcgcgagtg ttcgttccgt agtcgctggg cgccgaagca ggatcctcgc
      241 tggcgggtcc gagataggca accaaaatca ggagcaccag caaaattaga accagagtgg
      301 gaaaaagggc tctagctcga acattactca ttgaataggt cgaaactttc caaagtgtcg
      361 agagtcctac agcaaacagg gcaatgagaa cgatcccccg ggaaagcgtg aggacgaaca
      421 ccaaagcgac tattccgatg cctaagacga gaaataattg cataaaagca gccaaggcgc
      481 cagaggactt cttaatactg ctcgataacc aataaaaggc tagtatagca ctgatgagaa
      541 tcggtcccag aacatcgtca taatgaattg caggccaatc aacacggggt cggcttgtag
      601 tccaccaggt gccaatccca cccccaatgc ctgagaaatc gtacgtgaga caaaaataaa
      661 gagaaagtgc aaatgcaaaa caaaaagaaa gaaacgacaa aattgagaga ttttgatttg
      721 gctgggaact taaggagtaa tagagcaaaa ctgcgttaac gattagccag aatttaatac
      781 ccgcagccga tttatcataa gcagtccaat agccaaccca agctgtgaga agaaagataa
      841 tgatcatcca gtcaagcagg gtccattgaa taagagattg tcgcatgaag aacctgaata
      901 cccatgggag tagagcaatc aggctgaacc ctatcccaaa ctgtgggatt aacatccaga
      961 ccactgcact gatcattact aaaaagagat cagcgagagc aaaccaacga tgttcaacaa
     1021 gctgtcgcac gatatttttc tccagctgag ttttggattt ttatcctcct cccccaatag
     1081 gcaatccttc gtccgacgat gcccaggaaa ggtgttgaag accaacgttc agggccgttt
     1141 tcattgttga ccagtcatat agcaacatct ttaagtatgt aaacgctggc ccaggtcgga
     1201 acgcccattc acggaatgcc cgcttttgcc agtatagtaa acgttcagcc ggcatgttgg
     1261 gataatccaa caccgtgccc ttgtccatgt cgacgtgttc ccaacgtgta ccggggcgaa
     1321 accacttgtt cttgacgact tcaaagaaga agggtgttcc cgggtatggc gccgcaacat
     1381 gaaagagagc aatatccaaa ggcagtttct ttgcaaaatc aatcgtctgg cgaattgtat
     1441 cttccgtttc gccaggcagc ccgataatga agtagcccca gttcattatg ccagcttgct
     1501 tggcccatcg aagagcgcgt tccgcctttt caggatcggc acccttgcga gcatgcttga
     1561 ggatagcctc gttgccactt tcaatacccc aggagatcaa ccgacaaccg gccttgccca
     1621 tatacattag catttcttca tcaacgaagt ctacgcggct attgctcgtc cagtgaatgt
     1681 cgatcttctg ctcgatcatt aatttacaca tctcgatcac ctgatcgcgg ttgacggtaa
     1741 acaagtccgc atacatgtgg atgttgttga tgccaagctt cttcaaatac cacatttctt
     1801 cgagaatgag tttgggcgaa cgcaggcggg tggcatattg ataactcaca tgcttgatgc
     1861 aatacgtaca acctgcaggg caaccgcgac tggtaagcat aaaggtaaac ggtcccttga
     1921 tatatggcat gcggtatttt tgcagcggca gtaattcatg cattggaatg ggcatgtcgt
     1981 cgagatccgc cacaaatggg cgtgggaagt tgaggatgat ctcgccattc ttccgccagg
     2041 caatgccttt gatgccatgc atattgaccg ttccatcctc attaaacgat ggcttgtaca
     2101 tcagatcatg cttgtcaaag atcagctgta gctcaggaga acgctgatcg atcttatttt
     2161 ccaagtgatc caacagatcg cggatcgtga gatccggttc accgaccagg gcaaaatcca
     2221 aacttggaaa aggtcgcatc gtttccacgg ggatgggggt gatgtgcgtt ccaaaagcaa
     2281 ttgttttcgc tcctcgcgca tgggcgagga aacagccata catatcgttc tcgagtgtgg
     2341 gggccgtgag ctgcgtcaaa tagtattttg gctgatattg gtccagtagt tttgtaaact
     2401 ctgcccagcc catacgctca gcgttacagt ccacgacttt aactttatag acggggtgca
     2461 acaaagctgc catttgggcc agcgaaactt gaggccagac catgttctca cgtgtgcgac
     2521 gccctactcg atgctgggtg cggatccata attcaccgtc aggtgtagga ggattgacga
     2581 gcataatatc tactgcatcc gcagagcgtg gagcagagag aagtccatcg cggacgatgg
     2641 catccgcgtt gggaaaattc gccttatgta gcttaggcgt taagcgactt ccaagattta
     2701 cagatgcggg ttcattcgat ttcatttttc acctttccga aacatcaaca ataaaaaata
     2761 attaactcag tcccatgtct tgctttgtca gaaactctcg tttacttaat tcttccagga
     2821 ttcgaagcaa tatccaataa atactgagtt ggatgccagc caaaaacaac agggcgctgc
     2881 ccaataaata gaaccataag cgatcgattg tccagccttg aacgcccaaa gccagagaaa
     2941 tcactgcaat ggcgattccc aggacgaccg caaccactcc catccaacca aattgcaaat
     3001 ccaggggagc tttgaagatt ggcttaccaa ataatccctg ccggatcggt ttccgataga
     3061 agagcgacac aagataatta aatgtgacac ccagggcaaa tatgttaatg cctgcaacag
     3121 aggaaattag tgccaggaac aaagcagcaa caccccaggg tccgagggcg gtaatccctt
     3181 gcagtctggc aataacgaac cctaaaaaca ctagagccgc gatcccgatg ccggtcgcac
     3241 caatcatacc caaaatccgg accggattat acgaaagaac cgtccaaacc atactctgca
     3301 ggaaaatgcg tccatcgcga attacgctaa gctttgaccg accgacacgc tcgctgtaag
     3361 gaatcggcac ttcgccgatg tttatcccct cgtgtaaagc gcgcgtgctc atcactggtg
     3421 tcagattcag tccatctggc aacgggtaaa cttgctccag aatttcacgc ttaaagaccc
     3481 gcattccact ggcgctgtcc gtcacttttt ggcgacttaa taaagtgagc agtccggcaa
     3541 aaaagaagtt gcctatccgt cgtgtcagag gcatcttact ttcggcaccc gccattcgcg
     3601 atccgataat tagatcacct ccattcaacg cggcacggca aagctgcgga aaatactccg
     3661 gaggatacgt tccatctgca tccaaaaagc caatgagctc gcctcttgct tgagaaaaac
     3721 cagtcttgag tgccgcgcca tagcccttgt ttcgtggatg acaaatcaga ttcacgccgg
     3781 cgatcttcga tgcaacctca actgttcgat ccttagagcc gtcatccaca atcagcagtt
     3841 caagttcatc gacaccagca ttcttcaaat cgggacccac agaaaggacg cggtgcgcga
     3901 tttcagcaat acctttttct tcgttgtatg caggaataac aatagacaat agagtcatat
     3961 acttccatcc ttacctaact aaaaaacttg acgacctgat tgattgctct ggccatcaat
     4021 aactttgatg ggtttcgccg gtacaccacc cacaacagta tgcggcgcaa catccttggt
     4081 tactactgct ccagcagcaa taaccgcacc ctttccgatc ctgacaccgt cagtgacaat
     4141 agcgccagag ccgagccaaa catcatcttc gatcacaatc ccctgtgccg taataccctg
     4201 ctccacaaat ggacggttcg ggtcgtcaaa aacatggttg accgcaatga tttgagtaaa
     4261 cggtgaggta tatacgcggt cgccaatctg gactccaccc tgcccacgga tgaccgaata
     4321 ctcgccaatc aagctatcac gaccgattcg gataccggaa tgaggcaggt ttcgaaaatt
     4381 gtaaacatgt agaacagcac cgtgcattac tatagttcca tctccaatgt caataccttg
     4441 tggacaagca tgtaaataag cgccctgatc tatatagaca ccatggccaa gcctgatgtg
     4501 atcag
//
LOCUS       DASTJP010000511        12957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_554188, whole genome shotgun sequence.
ACCESSION   DASTJP010000511 DASTJP010000000
VERSION     DASTJP010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12957
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554188"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFQ23_14970"
     CDS             complement(<1..682)
                     /locus_tag="VFQ23_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907951.1"
                     /translation="MPTKKKSIAKKIPKEGAEQVTLSVEPGTKITITLDIGETVVDGK
                     VPLTVSIKQVQPQLTTVEESTSSEVQPVIETVAPVVAAVKPARHWGGFEALRSRLRVY
                     DLATWLFIFAVAVYLLTRLIGLTQFPIYFFTDEAIQSLSVMDLIENEYRDPRGVWFPT
                     YFRNGDYYNIGLSVYLQWLPAILFGKSAVATRAMSALVTLIAAIAVGLVLRDFFKLKY
                     WWTGTLFLS"
     gene            complement(735..1904)
                     /locus_tag="VFQ23_14975"
     CDS             complement(735..1904)
                     /locus_tag="VFQ23_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9907952.1"
                     /translation="MTDLALFSLSEEHKMIRDAARDFAQKEIVPIAAEFDESGEFPRD
                     TIKKMGGMGFMGIEIPEQYGGAGMDTLAYVLALEEICKADASHGVIMSVNNSLYCHGI
                     MKFGTEEQKQKFVIPVASGKAIGAYSLTEPQSGSDAGTMKSQAKRDGDHYVLNGRKSW
                     VTSGPVADYYVVFMMTDPHKKQKGVSAFLVEANTPGMTRGKKEPKLGIRASATSELIF
                     EDCRVPTENLLGKEGEGFKIAMTVLDAGRIGIATQALGIAEAAYEAARTYAVEREAFG
                     QPIGAFQGTGFKIADMKTRIEASRLLIYNAAMAKEKSKNSGGRYSLEASMAKLFASET
                     VMYVTHQAVQIHGGMGYSKELPVERYFRDAKITEIYEGTSEIQRLVISRSELGLK"
     gene            2032..2958
                     /locus_tag="VFQ23_14980"
     CDS             2032..2958
                     /locus_tag="VFQ23_14980"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET9907953.1"
                     /translation="MTTTSTAEERIWTVLSHISAVTFGMGILLPVIGWSEQRQKSKYA
                     SFQCLQALGYQSLGFTVWLLTYLVLVVVVLLTLIGSSSGVPTTAGSIDPLFSSALTVV
                     LILNFVLLALYAILPVIAAVACALGRDFRYPILGNRLAGYLKHDSSNEDPLSEDHEFR
                     WVAGMGHFSVIIALWGMLVPLAAWLTQGKQSLFLKFQSIQTLIFHAVALLLFFVAVFI
                     YLFGAILVFASMGLIESQFSASSSMIILILFGAISLIAVLIFFTIPLLHILGQWAGYR
                     VLKGEDYRYPLIGRLVDGWISKPAPNPPGETS"
     gene            2972..3793
                     /locus_tag="VFQ23_14985"
     CDS             2972..3793
                     /locus_tag="VFQ23_14985"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HET9907954.1"
                     /translation="MKILIATDMEGITGVTTWDQVTPGHAEYSRFRVLMTQDVNAAIR
                     GAMDAGADEVIIADGHWNGSNILVEELDPRARLNTGSPSPFSMMQGIDEDVDGVLFVG
                     YHARNGTPNAILDHTWSSSKVVNVWLNNLLTGEYGLNAGVAGHFGVPVIMVSGDQTAC
                     AQVAELLGDIETAVVKQATGRFAAECLAPQVAQELIYRAAERAVRRLKEGNMPKPFVV
                     DTPVRATIEFFTSDMADRATLIPFTEREGTRVSLTAPEMVLAYKGFRAMVTLASG"
     gene            4026..5237
                     /locus_tag="VFQ23_14990"
     CDS             4026..5237
                     /locus_tag="VFQ23_14990"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9907955.1"
                     /translation="MKSKWFALVSIAALVSIVLSACGATTGGEPGGPLQSVGEGEGEV
                     SIISWAGYIERGETDANFDWVTQFEADTSCKVTNKVAATSDEMVALMNEGGFDLVTAS
                     GDSSNRLIASGRVQELNLDLIPSYNTIDPRLQNAPWHTVDTDGDGTAEHYGVPYSSGQ
                     NILMYNTEIFGDQPPTSWDVVFQEMTLPDGETNSGRIQAYDGPIYVADAALYLKYHQP
                     ELGIDDPYSLTREQFDAAINLLREQRKLINRYWHDAFIQMDDFTNEGVAASGSWPFQA
                     NLLKGSGAPIEKVVPIEGATGWADSTMLHVDAPHPNCAYKWLEWSLNKKLQGDLAAWF
                     QNIPVRLDACDGNELLGADGCVNNGLDNFDKIVWWRTPTSDCGDGAGGKEDCVPYHEW
                     VTNYVAVIGGR"
     gene            5311..6348
                     /locus_tag="VFQ23_14995"
     CDS             5311..6348
                     /locus_tag="VFQ23_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907956.1"
                     /translation="MTNSSIPAIRFDKVSRHFGEVKAVDQADFEIRDGEFFSMLGPSG
                     SGKTTCLRMIAGFDRPTSGSIFLYGQDVSDLPPYERDVNTVFQDYALFPHMSVGDNIA
                     YGLMVKKVPKPERMKRVDEMLDLVRLSGFAGRKPAQLSGGQRQRVALARALINHPKVL
                     LLDEPLGALDLKLRQQMQVELKAIQQRVGITFIFVTHDQEEALTMSDRIAVFNEGKIQ
                     QIGTPVEIYERPASPFVAGFVGTSNLVSGEVAKRITGSEQMFSIRPEKIHLGSVNGKS
                     EDEMLFVDGVVRDVVYLGLFTRYLVEIEGGSDLVVVEQNLKTTSMDVLSVKGQKVRLF
                     WDKAHISRVGG"
     gene            6350..7255
                     /locus_tag="VFQ23_15000"
     CDS             6350..7255
                     /locus_tag="VFQ23_15000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9907957.1"
                     /translation="MLTRISSYLYRRPGLVLALLLGPPLVYMLVVYLGSLFNLLINSF
                     FYLEEFTGLIVREFTLRTYLQIFSPANREIFLRTATMATIVTLVDALIAFPLAYYIAK
                     FTSPRLKTWLLIAVTIPLWSSYLVRVYAWKLILAKEGILSWFINLFQLDGVLQWLLSF
                     ESIGGSSLALSPIGMFIVFVYIWLPYMIIPIQTSLERVPGSLLEASSDLGAKPGQTFR
                     NVILPLAFPGVIAGSIFTFSLTLGDFIIPLVLGNSKFFLGQAVFSYQGTGGNIPLAAA
                     MTMGPVVLMIIYLLVARKLGAFDAL"
     gene            7245..8060
                     /locus_tag="VFQ23_15005"
     CDS             7245..8060
                     /locus_tag="VFQ23_15005"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9907958.1"
                     /translation="MPSNSGQKASLGLKIAALGTLLFLHVPMFIIFLYTLTPDETTYT
                     FPLPGITTKWYGIAFQRSDLWAALTLSLQVAAVATVVALVLGTLAAAAVYRSNFFGRE
                     AISFLLVLPIALPGIVTGIALRSAIGGFNIPFSYWTIVIGHATFCVVVVYNNVLARFR
                     RMQGSQIEASMDLGANSFQTFRYVVLPNIATALLAGGMLAFALSFDEVIVTTFTAGQQ
                     STLPIWIFSQLTRPRDRPVTNVVATFVILITFIPIFFAQRISAQSSDTEGETK"
     gene            8499..9029
                     /locus_tag="VFQ23_15010"
     CDS             8499..9029
                     /locus_tag="VFQ23_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET9907959.1"
                     /translation="MTGQFHSYLNPKCDSGTFEEKGGCGVFAREPIKKGELLGLFGGR
                     IAARDELDPTMPNFTQRVLQVEDDFYLITPDPLEPSDCFNHSCEPNAGFTGQIGIVAM
                     RDIKAGEEINFDYAMCDSTPYDEFPCQCGSERCRGSVKGNDWMRHELWEKYEGYFMPY
                     LARRIEALKAQTRIRA"
     gene            9113..10570
                     /locus_tag="VFQ23_15015"
     CDS             9113..10570
                     /locus_tag="VFQ23_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907960.1"
                     /translation="MFRIYIIALVLRLVPILLARGLGIGLDDMFQYDMLARSLAEGNG
                     FRWYAQTDLNQLASFVDFDLSTVREYDPILGIPTSFRAPLYPTFLATVYFFAGTEFSR
                     FFAARLAQAIFLGAPLAPLTYLVSRKLFPARLVGEGSGVRSEPAARTAAWVVAAYPML
                     LVYPLGLGTENPFFVLLLAAFLFLLKTVNQPTPLNFLLAGFFLGLTALTRSVILPFAV
                     LAILWVWFMLYQHRGAILIVITLALTIFPWVIRNSILHHKMTGIETSMGYNLYLGYHP
                     KGNGSFVFGPSLDLLTILDDAERDKIGTQKAIEFIQAQPERFMPLAVNRLGYFFGLEK
                     RVLMYFYSNNIIGYIPLPWLLTISAFLLLPFAIISVSAALSLALLHRRPEHVLLILLF
                     VAYVLPHVFILSEDRFHLALVPYFSILAAQVWIRGFDQLSDRWKESAKGKLLVAFAVL
                     AAVFLVMNWETELLRDATKIAQLLGPNGNQSYFPY"
     gene            10577..11191
                     /locus_tag="VFQ23_15020"
     CDS             10577..11191
                     /locus_tag="VFQ23_15020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9907961.1"
                     /translation="MKYLLGEKLTFDWKVVTITIVSTLLLMVDYYHRLTPYKYWDRVI
                     LYLVIPLLIILLIFRENPKEFGFSLGDWKAGLLMTAIGILFMAPVIYFLGHGNESMKS
                     YYEPFVNGLPWTTFLDLIGWEFLFRGWILFGYARKFGPESLWLQAVPFALMHNGKPEV
                     ETLSTIFGGFAFGWVAYRTKSFLWPFLIHWFIATFIIIVAAGLI"
     gene            11207..11650
                     /locus_tag="VFQ23_15025"
     CDS             11207..11650
                     /locus_tag="VFQ23_15025"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9907962.1"
                     /translation="MTNYTLRPARESESRAIKELIHEVGINPMGVDWKRFIVAVDAQD
                     QIIGIGQLKPHGKDILELASIGVYPEYRGQGIARAIIETLLKDSPRPLYLMCESKNGG
                     LYEKFGFLAISYEEMPRYFQRISKLAGLASTLARHDERILIMKLQ"
     gene            11669..12241
                     /locus_tag="VFQ23_15030"
     CDS             11669..12241
                     /locus_tag="VFQ23_15030"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HET9907963.1"
                     /translation="MRSVLYMASGILLGLFLAVLVWVVARNPTGEPVILRPVPTEKPI
                     IVHITGAVPRPGVYALPQGARVQDAISAAGGFLAEAEKSQINLARLIEDGEKFDIPYI
                     EGASPVIPTPGETVVAATTELIDINIASQAELESLPGIGPTTAQKIIDYREQNGPFLA
                     IEDIVNVSGIGPGTYERIKDLITVTTSTGP"
     gene            complement(12305..12871)
                     /locus_tag="VFQ23_15035"
     CDS             complement(12305..12871)
                     /locus_tag="VFQ23_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907964.1"
                     /translation="MLRRLWTVVVVLILVTIFASSVGAAASENKSVTLISSTFNSGTG
                     IVLLFQTSGLTQKDLKSGSVFAHSNEYRMSCNFKDDSTVVRCVIPGGLTQYAGESFSG
                     TLAGFRFWSQIPERKADELTCPEGQSLWYTINVYFDGELSESGDIPVEFYGFLLDLLA
                     SFPEEFEGITLEIAGQYCGEEVILEIPE"
BASE COUNT         2963 a         3136 c         3188 g         3670 t
ORIGIN      
        1 tcgacaaaaa taatgtccct gtccaccaat atttcagttt gaaaaaatct cgcagaacca
       61 gcccgacggc gatcgcagca atcaaggtca ccagggcaga catggcccgc gttgccacag
      121 cggattttcc gaaaaggatg gcaggtaacc attgcagata aacacttaat ccaatgttgt
      181 aataatcacc attcctgaaa taagtaggaa accaaacacc ccgtggatct cggtattcat
      241 tttcgatcag atccatgacc gataaggatt gaatcgcttc atcggtaaaa aagtaaatgg
      301 gaaattgggt cagtccaatc aggcgagtga gaagatagac agcaacagca aaaataaata
      361 gccacgtggc aaggtcgtaa acgcgcagcc ggcttcgcag tgcctcaaaa ccgccccagt
      421 gtcgggctgg tttgacagca gccactacag gtgcaacggt ttcaattacg ggttgaactt
      481 cagaggacgt gctctcttca accgtagtca gttgtggttg aacctgcttg atgctaacgg
      541 taagtggcac tttcccatcg acaaccgttt cgccaatatc cagagtgatt gttatcttcg
      601 ttcccggttc aacagacaaa gtgacttgtt ctgcaccctc cttgggaatt tttttggcga
      661 ttgatttttt ctttgtgggc atgagttgcc ttgacctcac cttgtatcac ttcgcaaatc
      721 catcaatcga ataattattt caatcccaat tcacttctgg atatcaccaa ccgttgaatt
      781 tcacttgtcc cttcatagat ttcagtgatt ttggcatccc ggaagtatcg ttccaccggt
      841 aattccttgc tatagcccat accgccgtgg atttgaacag cctggtgagt cacatacatg
      901 actgtttcag aggcaaagag tttcgccatg gaggcttcta gcgaatatcg tccaccgcta
      961 ttcttcgatt tttcctttgc catagcggcg ttgtagatca atagacggga agcttcgatg
     1021 cgtgttttca tatcggcgat cttgaaccca gttccctgaa acgcgccgat gggttgacca
     1081 aacgcctctc gctcgaccgc gtacgtgcgt gcggcctcgt aggctgcttc cgcgatccca
     1141 agggcctgcg tcgcgattcc gatgcgacct gcatccagga ccgtcatggc gatcttgaag
     1201 ccctcgcctt ctttccctaa aaggttttct gttggtacgc ggcaatcttc gaagattaac
     1261 tcacttgtcg cagaagcacg gatgcccagc tttggctctt tcttcccgcg tgtcattccc
     1321 ggggtatttg cttctaccag gaacgcacta acgccctttt gcttcttatg cggatccgtc
     1381 atcatgaaga ccacgtagta gtcggcaacc gggccgctcg tcacccagga ctttctccca
     1441 ttcaatacat aatgatcccc atcgcgcttg gcctggcttt tcattgttcc ggcgtcagag
     1501 ccgctttgag gctcggtaag tgagtaggca ccgattgctt ttcctgaagc tacgggaatg
     1561 acaaatttct gcttttgttc ctctgtgcca aacttcatga tgccatggca atacaacgaa
     1621 ttgttgactg acataatgac cccgtgtgac gcatccgctt tgcagatctc ttcgagtgca
     1681 aggacatacg caagcgtatc catgcctgct ccgccatact gctcagggat ttcaattccc
     1741 atgaatccca ttccacccat ttttttaatg gtgtcgcgtg gaaattctcc gctttcatca
     1801 aattccgctg cgatcggcac gatttctttt tgcgcgaaat ctcttgcggc atcgcgtatc
     1861 atcttatgtt cttcactgag agaaaacagt gcaaggtcgg tcatctcgcc tccagaaaaa
     1921 gttcgtttcg cgattatacc gcctggcggt cgctcacaga accgcctgat ttccctctga
     1981 taaaatacac aagagcgcgg cgttgctctc tcagacaagc cttaacagac tatgactact
     2041 acatcaaccg ccgaagaacg tatctggact gtgctttcac atatttccgc ggtcacgttt
     2101 gggatgggta ttctgttgcc ggttatcggc tggtcggaac aaaggcagaa atcaaaatac
     2161 gcctcttttc agtgtttgca ggcattaggc tatcaatcgc tggggtttac cgtttggctt
     2221 ctgacgtatc ttgttttggt cgttgtggta ttgctgactc tgattgggtc aagttccggg
     2281 gtgccaacca cagccggctc tattgatccg ctttttagct cagccttgac cgttgtcctg
     2341 attttgaact ttgtgcttct tgctctttac gctattttgc cagtcatcgc ggccgtggcg
     2401 tgtgcccttg gcagggattt tcgctatcct attttgggaa atcgacttgc aggttatttg
     2461 aaacatgatt cttccaatga agacccgctg agcgaagacc atgaattccg ctgggtggca
     2521 gggatgggac atttcagcgt tatcatcgcg ttatggggaa tgctggttcc tctggctgcc
     2581 tggctcactc aggggaaaca aagtttgttt ttgaaatttc aatccatcca gacgctgata
     2641 tttcacgcag ttgcactgct tctctttttt gttgctgttt ttatatactt gtttggcgcc
     2701 atattggtat ttgcgtccat gggattgatc gagtctcaat ttagtgcatc atcgtccatg
     2761 atcatcctga tccttttcgg tgcgatttca ttgattgcag ttttaatttt ctttacaata
     2821 cctttacttc acattctggg gcagtgggcc ggctatcgtg tcctaaaggg cgaagattat
     2881 cgataccccc tgattggaag gttggtggat ggatggattt ctaaacctgc gcccaacccg
     2941 ccgggcgaaa cctcatagag gagaaacttg catgaaaatc ctgatcgcga ctgacatgga
     3001 agggataaca ggtgttacca cctgggatca agttacgccc gggcatgctg agtactcccg
     3061 ttttcgagtg ttgatgactc aggacgtaaa tgcagccatc cgtggagcaa tggatgctgg
     3121 cgcagacgaa gttatcattg ccgatgggca ttggaacggc tctaatattt tggttgaaga
     3181 actcgacccg cgggctcgac tgaacacagg ttcaccctct ccattttcca tgatgcaggg
     3241 aattgatgaa gatgtggatg gtgtactttt tgttggctat cacgcccgca acgggacgcc
     3301 aaatgccatt ctggatcaca cctggtcttc aagtaaagtt gttaatgtct ggttgaataa
     3361 cttgttgaca ggggagtatg gcttgaacgc aggggtcgcg ggtcattttg gggtgccggt
     3421 cattatggtc tcgggtgatc agacggcctg tgcgcaagta gccgagcttc taggtgatat
     3481 cgaaactgca gtcgtcaaac aggcgacggg tcgctttgcc gcggagtgct tagcaccgca
     3541 agtagctcaa gagttaattt accgcgcagc ggagcgtgcc gtacggcgct tgaaggaggg
     3601 caacatgccc aagcccttcg ttgtagatac acctgtgcgg gccacaattg aattctttac
     3661 ttccgatatg gcagatcgtg cgaccctcat cccgtttaca gaaagggaag ggacgcgtgt
     3721 ctctttgact gctccggaaa tggttttggc atataagggg ttccgcgcca tggtgactct
     3781 ggcatcaggc tgagataatg tgtattaaat ggtgacacgc gtcggtttgt tgggaactga
     3841 tatagccatt tgcatcaaaa tcggaaattc ctgattgacg ttccacccta gttctcaata
     3901 taattgaact acaatgcagt ttccatagat atcctttatc tgattgagag gaggtgcccc
     3961 atcacgcaaa gtcaacggct cgtttgttcg cattgatgat caaacaaatt tccaaggaga
     4021 gagaaatgaa gtcgaaatgg tttgctcttg tatcaattgc tgcgcttgtc agcattgtgc
     4081 tttctgcatg tggtgccacc accggaggtg aaccgggtgg cccgctgcaa tccgttggcg
     4141 aaggtgaagg ggaagtctcg attatttcat gggccggtta tatcgaacgc ggagagaccg
     4201 atgctaattt tgactgggtc actcaatttg aagctgatac cagctgtaag gtgaccaaca
     4261 aagttgctgc aacttccgat gaaatggtcg ctttgatgaa tgagggtggt tttgatctgg
     4321 taacggcctc tggtgattcg tcgaatcgtc tgatcgcttc tggtcgtgtt caggaactca
     4381 atctcgattt gatccccagc tacaacacca ttgatcctcg cttgcagaat gccccctggc
     4441 acaccgtgga cacggatggg gatggcactg ctgagcacta cggtgttccg tattcctccg
     4501 gtcagaatat tctgatgtat aacactgaga tcttcggcga tcagcctccc acaagctggg
     4561 atgtggtatt ccaggaaatg actctgccgg atggtgaaac gaattcgggc cgcatccagg
     4621 cctatgatgg tccaatctac gttgccgatg cagctcttta tctgaaatat caccagcccg
     4681 agcttggcat tgacgatccc tattcattga cccgcgagca gttcgatgcg gcgatcaatc
     4741 tccttcgcga acagcgcaaa ctgatcaacc gctactggca cgatgccttt attcagatgg
     4801 atgatttcac caacgaaggt gtggcggctt ccggttcatg gcccttccag gctaacctgc
     4861 taaagggtag cggcgcgccg atcgagaagg ttgttcctat tgaaggcgca accggctggg
     4921 cagactcaac catgctgcat gtggatgcac ctcaccccaa ttgcgcttat aaatggctgg
     4981 agtggtccct gaacaagaag cttcagggtg acctggcggc ctggttccag aatattccgg
     5041 ttcgcctgga tgcctgcgat ggcaatgagc tacttggtgc tgatggctgc gtgaacaatg
     5101 gtctggataa ctttgacaaa atcgtctggt ggcgcactcc cacttcagat tgtggcgacg
     5161 gagcgggtgg gaaagaggac tgtgttccct accacgagtg ggtcacaaac tatgttgctg
     5221 tgatcggtgg tcgttagtct atcgttttaa catcaggcgg gcgtcaagcg tcccgcctga
     5281 tgtttttacc aacgaaacta catcagcctc atgaccaatt catcgatccc tgcgattcgt
     5341 tttgataaag tcagccgcca ctttggggag gtgaaagccg tagatcaagc cgattttgaa
     5401 atacgtgacg gcgaattttt ttccatgctg ggcccttccg gctccgggaa aacaacctgc
     5461 ctgcgcatga tcgccggatt tgacagaccc acctcgggga gtattttttt atacggacag
     5521 gatgtttccg acctgcctcc ctatgaacgg gacgtaaata cggtattcca ggattacgca
     5581 ttgtttccac acatgagcgt cggcgataac attgcctatg gtctcatggt gaagaaagtg
     5641 ccaaaacccg aacgaatgaa acgcgtggat gagatgctgg accttgtccg cctttccgga
     5701 tttgccggac gaaagccggc tcaactctct ggcggacaac gtcaacgtgt ggccctggct
     5761 cgggcgctga tcaatcatcc aaaagttctc ctgctggatg aaccattagg tgccctggat
     5821 ttgaaactgc gccagcaaat gcaggtggaa ctaaaagcca ttcaacagcg agtcggtatc
     5881 acctttatct tcgtcaccca cgatcaggaa gaagcactga ccatgagtga ccgtatcgca
     5941 gttttcaatg aggggaaaat tcagcagatt ggaacgcctg tagaaatcta cgaaagaccc
     6001 gcttcgccgt ttgtcgccgg gtttgttggt acgtccaatc tggtgagtgg ggaggtagcg
     6061 aagcgcatta ccggttcaga gcagatgttc tcgatccgtc cggagaaaat ccatctgggg
     6121 tcagtgaacg gcaaatcgga agatgaaatg ctctttgttg atggcgttgt aagagatgtt
     6181 gtatatcttg gcttgtttac acgttatctc gtagagatcg aaggtgggag cgatctggtt
     6241 gtggttgaac aaaatcttaa aacgacttcc atggatgtat tgtcagtaaa aggtcagaaa
     6301 gtacgtctct tctgggacaa agcccacatc agccgggtgg ggggctaacg tgttgacacg
     6361 catctcaagc tatctttacc gacgccctgg tttggtgctt gcccttttac tgggtccacc
     6421 cctagtctat atgctggtgg tgtatctcgg ttcattattt aatttattga tcaatagttt
     6481 tttctatctc gaagaattta ccggtttaat tgtccgcgaa ttcacattaa ggacctatct
     6541 tcaaatattc agccctgcaa atcgcgagat ttttcttaga accgccacca tggctacgat
     6601 cgttacgctg gttgatgcct tgatcgcttt tccgctggca tactacatcg caaaatttac
     6661 ctcgcctaga ttgaaaacct ggcttctcat cgcagtcaca attccactct ggtcgagtta
     6721 tttggtgcgg gtctatgcct ggaagctgat cctggcaaaa gaagggatat tatcctggtt
     6781 tatcaatctg tttcaactgg acggggtttt acagtggctg ctgagctttg aaagtattgg
     6841 tggttcatct ctggctctat cacccattgg tatgttcatc gtttttgtat atatctggtt
     6901 gccctatatg atcataccga tccagacctc gctggaacgg gtgccaggtt cattgctcga
     6961 agcctccagc gatttaggcg ccaaacctgg gcagacgttt cgcaatgtga ttctcccgct
     7021 ggccttcccg ggtgtgatag caggctcgat ttttaccttc tcattaacat tgggtgattt
     7081 cattatcccc ctggtgcttg gcaattcgaa gttttttctt ggtcaggccg ttttttcgta
     7141 tcaaggaacg ggcgggaata ttcctctggc ggctgccatg acgatgggtc ccgtggttct
     7201 tatgatcatt tatcttcttg ttgctcgcaa actcggagca ttcgatgccc tctaattctg
     7261 gtcagaaagc ctcattagga ttaaaaattg cggcgcttgg cacgttgttg ttcctgcatg
     7321 tgccgatgtt cattattttt ctttatacac taactccgga cgagacaacc tacacattcc
     7381 cgctcccggg aatcacgacc aaatggtatg gcatcgcctt tcagcgcagt gacctgtggg
     7441 ccgcgcttac gttaagcctt caggtggcgg ctgtagccac ggtcgtcgcg cttgttctgg
     7501 gtaccctcgc tgccgctgct gtatatcgca gcaatttctt cggtagagaa gcaatttcat
     7561 ttttgcttgt cttaccaatc gccctgcccg gcatcgtgac cggaattgcg ctgcgcagcg
     7621 caattggtgg atttaatatt ccgttctcct attggacaat tgtgatcggt catgccactt
     7681 tctgtgttgt ggttgtatac aacaacgtcc tcgcgcgctt tcgccgaatg cagggatcac
     7741 agatcgaagc ctcgatggat ctgggtgcga attcatttca aacgtttcga tatgttgttt
     7801 tgccaaacat cgcaactgcc ttattggccg ggggcatgct tgcgttcgcg ctttcctttg
     7861 atgaggtgat tgttactacg ttcactgctg gacaacaatc caccctgccc atttggatct
     7921 tttcgcagtt gactcgtccg cgtgatcgtc cagtcacaaa tgtagttgct acgtttgtta
     7981 tcctcattac ctttattccc atcttctttg cacaacggat ctctgcgcag agctcggata
     8041 cggaagggga gacgaagtag tgagctgcag cgacagtcac ctttgcgcgg cactcccgaa
     8101 gtgaaaagaa gggacaaaat gacagtcctt ttttgataga gaatccccac aatttcctaa
     8161 tctgcaaaga ttgacatcac gtcaataccc attacaattg cattcaactt taaaaaggag
     8221 cgttcgaaat tctaacgact cgcacgagtg tgaatgaaga aatgcttgtg cactttatct
     8281 aaatatcgcc acgaataaaa tctggcttcc gccgccctcc aactaagact tggtgaggcg
     8341 gcgggactgc ttaacgtgat tgcagtcaac acagtggttc agagctgttg tgctttggct
     8401 gtttttgttt gtctgtttac gaaaaacgtg ttgtcacttt ggcgaaagtt gccaaagata
     8461 ttctgggcga aatgccttaa ccaaaacgaa aggaaagaat gactggtcaa tttcactcct
     8521 atttaaaccc gaagtgtgat tcgggcacct tcgaagagaa gggcggatgc ggtgtgtttg
     8581 cccgcgagcc aattaagaag ggcgagttgc tgggcttgtt tggcggccgg attgcggcac
     8641 gagatgaact cgatcctacg atgccaaact tcacgcaacg agttctccag gtcgaagacg
     8701 atttttactt gatcactccc gacccattgg agccttctga ttgcttcaac cattcctgtg
     8761 agccaaatgc cgggtttacc ggtcagattg gaatcgtcgc catgcgggat atcaaagcgg
     8821 gcgaagagat caattttgac tacgccatgt gtgacagcac accgtatgat gagttcccgt
     8881 gccaatgtgg cagcgaacgt tgccgcggca gtgtcaaagg gaatgattgg atgcgccatg
     8941 aactatggga gaaatatgaa ggttacttca tgccctatct cgcaagacgc atcgaagccc
     9001 tcaaggctca aacgcgcatt cgagcataaa ctcggtgcgc ggcagttcat cgcagtatag
     9061 aaaacggatt gcacggtaaa atggtgcatt ccgttttatg tttaattcgt ttgtgtttcg
     9121 aatttatatc attgcactgg ttcttcggtt agttcctatc ctgcttgcac ggggattggg
     9181 gattggactg gatgatatgt ttcaatatga catgttggcc cgcagccttg cggagggaaa
     9241 tggattccgt tggtacgcgc agacagactt aaatcaactc gcatcgttcg tggactttga
     9301 cctgtccact gttcgtgagt atgatcccat ccttggtatc cccacttcat tccgcgcgcc
     9361 gctatatccc acttttttgg ccaccgttta ttttttcgca gggacggagt tttcacgatt
     9421 ttttgctgca cgcctggctc aagctatctt tctcggtgcg ccgctggcgc cgctgactta
     9481 ccttgtttcc cggaagctgt ttcctgctcg ccttgttggc gagggatcag gtgtaaggtc
     9541 cgagcctgct gcacgaaccg ccgcatgggt tgtggctgca taccctatgt tgcttgtcta
     9601 cccactggga ttgggaactg aaaatccttt ttttgtactc cttctcgcag cttttttgtt
     9661 ccttcttaaa actgtcaatc agccgacccc acttaacttt ttactggcag gtttttttct
     9721 cgggctcaca gccctgacgc gttcggtcat actcccgttt gctgtgttgg cgattctttg
     9781 ggtctggttt atgttgtacc agcatcgtgg tgcgattttg atcgtaatca ctctggcatt
     9841 gacgatcttt ccgtgggtga tccgcaactc aattctgcat cataaaatga caggtatcga
     9901 gacctccatg ggctataact tatatctggg ttatcacccc aaaggaaatg gctccttcgt
     9961 ctttggtcct tctttagatt tattgactat tcttgatgat gccgaacgag acaaaattgg
    10021 tacacagaaa gcgattgaat tcatccaggc gcagccagag cgttttatgc cgcttgctgt
    10081 aaatcgtctc ggttatttct ttggtcttga aaagcgtgtg ttgatgtatt tttattccaa
    10141 caatattatc ggatatattc ctcttccatg gctgttgacc atctcagcat ttttactttt
    10201 gccttttgca atcatatccg tctctgcggc actgtcactg gcactgctcc accgccggcc
    10261 cgagcatgtt ttgttgatcc tgttatttgt tgcttatgtg ttgccacatg tgtttattct
    10321 ctccgaggac cgatttcacc tggcattagt accctatttc tctattctcg cggcccaggt
    10381 ttggatcaga ggctttgatc agctgtcgga tcgctggaag gagtctgcta agggcaaatt
    10441 gctggttgcc ttcgcagtgc ttgctgcggt ttttctggtc atgaactggg aaacggagct
    10501 gctccgcgac gcaacgaaaa tcgcacagtt attgggtccc aacggaaatc aatcttattt
    10561 cccctattga gcaatcatga aatatttact cggcgaaaaa ctgacttttg actggaaggt
    10621 tgtcaccatt accattgtca gcaccctcct gctcatggtg gattattatc atcggctgac
    10681 accatacaag tattgggatc gcgtgatctt atatcttgtg atccctctgt taataattct
    10741 ccttattttt cgcgaaaatc caaaagagtt tggattttct cttggcgatt ggaaagccgg
    10801 attgttgatg accgcgatcg ggattctttt catggctcct gttatttatt ttctggggca
    10861 tggtaatgaa tcgatgaagt cttattatga gccgtttgtg aatggcttgc cctggacaac
    10921 atttctcgat ttgatcggtt gggagtttct ttttcgcggc tggattctct ttggatacgc
    10981 gcggaagttc ggtccagaat ccttgtggct gcaggctgtt cctttcgctt tgatgcacaa
    11041 cggaaaaccg gaagtcgaaa cgttgtccac gatctttggc ggctttgcat ttggatgggt
    11101 agcctaccgg acaaaatcat ttctttggcc atttctaatc cactggttta tcgccacgtt
    11161 tatcattatc gtcgccgcgg gattgatcta gggcctggaa aaacagatga cgaattacac
    11221 actccgcccc gcgcgtgaat cagaatcacg cgctatcaaa gaactgattc atgaagttgg
    11281 gatcaacccg atgggcgtag attggaagcg ttttatcgtc gccgtcgatg cacaggatca
    11341 aattatcgga ataggtcagt tgaaacctca cggtaaggat atccttgaac tggcttcgat
    11401 tggtgtttat cctgaatatc gcggtcaagg aatcgctcgg gcaattatcg aaactctgct
    11461 gaaagacagc ccacgtccgc tgtacctgat gtgcgaatcg aaaaatggtg gtctgtatga
    11521 gaaattcggg tttcttgcaa tttcttatga ggagatgcct cgctacttcc agcggatcag
    11581 caaattagcc gggttggcta gtactcttgc acgccacgac gagcgcattc taattatgaa
    11641 gttgcagtaa aattagctca aggaaaatat gagatctgtc ctttacatgg catcgggaat
    11701 tctattgggt ttgtttcttg cggtgttggt ctgggtcgtc gcgcgtaatc caaccggcga
    11761 gccagtgata ttgcgtccgg tgcccactga aaaacccatt attgttcata tcaccggtgc
    11821 ggtgccacgc ccgggcgtat atgcccttcc acagggtgcg cgtgtgcagg atgcgatttc
    11881 agcggcgggt gggtttcttg ctgaagctga aaaatcccaa atcaatcttg cgcgtttgat
    11941 tgaagatggc gaaaaattcg atattcctta catcgaaggt gcgtcacctg ttattccgac
    12001 ccccggtgaa actgttgtgg cggcgacgac tgaacttatc gatatcaaca tcgcttccca
    12061 ggcggagctc gagtcgttgc ccggtatcgg tccaacgact gcgcaaaaaa tcattgacta
    12121 ccgcgagcaa aatggaccgt ttcttgcaat tgaagatatt gtcaatgtct ctgggatagg
    12181 gccgggcacc tatgagcgta tcaaggactt gattactgta acgacatcca caggaccgta
    12241 ataggaatcc atataacaaa cagcccgact ttctaagtcg ggctgtttgt attaaacatt
    12301 gaatttattc tggaatttct aaaatgactt cttcgccaca gtattgcccg gcgatttcca
    12361 gggttatacc ttcaaactcc tccgggaagc ttgcaagtag gtccagcaag aacccataaa
    12421 actcaactgg aatatctccg gattcactga gttcaccgtc gaaatacaca ttgattgtgt
    12481 accataaaga ttgaccctcc gggcaggtta gttcgtctgc ttttctctcg gggatttggc
    12541 tccaaaagcg gaaaccagcc agcgtgccgc tgaagctttc gccagcatac tgagtcagcc
    12601 cgccaggaat tacacaacgg acaaccgttg aatcgtcctt gaagttacag gacatgcgat
    12661 attcattcga atgcgcaaat actgagccac tcttgagatc tttttgggtt agcccgcttg
    12721 tttggaacag gagcacgatg ccggttccac tattgaacgt agagctgatc aacgtcacgc
    12781 ttttattttc agaagcggca gcccccacag acgaagcaaa aattgttacc agtataagga
    12841 ctacaacaac ggtccacaac cgccttaaca tagggcactc tcctgattag atcgaattaa
    12901 gatcaggacg aagcggctct gggcaagtta ccagaaaggt aactctttgc attacag
//
LOCUS       DASTJP010000512         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_863138, whole genome shotgun sequence.
ACCESSION   DASTJP010000512 DASTJP010000000
VERSION     DASTJP010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863138"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..>2181
                     /locus_tag="VFQ23_15040"
     CDS             327..>2181
                     /locus_tag="VFQ23_15040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9907965.1"
                     /translation="MKSFSFITKVYLILTYCAGSVIFAAHVGRVNLENPWLLLVLCVI
                     ASLTLILKVEGSTNRSHYTFNFLVYGFTFAVFGTSEAILVIVVSNIVEWIWSRPPWFI
                     QLFNAASYLIVMQAASAVYEWFNPAHSIDTWQTALALIFSMATFNLLNHLMVGVIIWF
                     ARGETFRKSGVFDFFPLMLDLSLLYFGASLSIVWNHNALALGLFLVPLYLIYGTLRVP
                     ALERKTEIDSKTGLFNHEYFRNQLNNELPRAKRFDRPLCIVLADLDLLRNINNTYGHL
                     AGDEVLIGVAKVLKAAVRDYDVVCRFGGEEFAILLPETTVAQAYERAELIRKTVEEME
                     FTVPTSVTSIRATMSFGIAQREGFSQTADEIIHNADLALYHSKLSGRNRTHAYSEDAY
                     VDFSTNEPSSHAEPVQEEPESTQQAADLGKSDRTMAALESDASSGTDAAQATPQAHRE
                     KPQKPDYAVGIFIGLLALISVLSLAAVVSWMSLLETGAPFDWPGLLVLSVLIVVSEGF
                     SIDLYFRQNSVSTSALPILVAYLVFGPIGTVSASLILAIALMIKYRSRFSRFVFNFSN
                     HLLAGAFALAMVMLAGGNFIQLSPIYQILLSLASAAILYLTTTSMIAFGMSL"
BASE COUNT          459 a          552 c          573 g          597 t
ORIGIN      
        1 ggtaagagtt tccggaatga tccttttagg ggttgtagtg agcctggcgg tattcatgct
       61 ggtgtacgct cttcaaaacc ctgacttcat ctggttatta ggcactgtga gttggaacgg
      121 ttagtgtttg aggttcctgg aggcaaagcg cttccaggaa cctgtaatgc gatagtaaag
      181 tgcggtcggt gctgacgagc cctacaatca tctggactgc gcgtactgta acgtcgtctc
      241 agatggccgg ctcattcccc tccatggcat tatccagttg tagaagtaaa taccgcgtga
      301 cttcgtctta atggcaggaa gatccaatga aatcattttc tttcattacc aaagtgtatt
      361 taattctgac ctactgcgcg ggcagtgtga tcttcgccgc acatgtaggc cgggtaaatt
      421 tggaaaatcc atggttactg ctggttctct gtgtgattgc ctccctgact ttgatcctga
      481 aagtggaggg atcgacgaat cgatcccatt acacattcaa cttcctggtg tacggtttca
      541 cattcgcggt gtttggaaca tctgaagcca ttctagtcat tgtggtatcc aatattgtgg
      601 aatggatctg gagcaggcca ccctggttca tccagttgtt caatgccgca agctacctca
      661 tcgtgatgca ggcagccagt gccgtgtacg agtggttcaa ccccgcccac tcaattgaca
      721 catggcagac ggccctggca ctcatcttca gcatggcaac gttcaacttg ctgaatcacc
      781 tgatggtggg agtcataata tggtttgccc ggggcgaaac cttcaggaaa tccggtgtct
      841 tcgatttctt ccctctgatg ctcgacttaa gtctgcttta tttcggtgcc agtctctcca
      901 ttgtatggaa tcacaatgcc ctcgccctgg gcttgttcct ggtgccactt tacctgatct
      961 atggcacgct acgcgttccg gctttggaac gcaagaccga gattgacagc aaaaccggac
     1021 tgtttaacca tgaatatttc aggaaccagt tgaacaatga acttccgcgt gccaagcgct
     1081 tcgatcggcc tttgtgtatt gtccttgccg acctggactt gctgcgcaat atcaataaca
     1141 cctacggtca tcttgcaggc gatgaggttt tgatcggagt ggcgaaggtg ctgaaagccg
     1201 ctgttcgtga ttacgatgtg gtgtgccgct ttggcggcga ggagttcgca atactcctgc
     1261 ccgaaacgac agttgcccag gcgtatgagc gtgccgagtt gatccgcaag accgtagagg
     1321 agatggagtt cacagtcccg accagcgtga cttccattcg tgcgaccatg agtttcggaa
     1381 ttgcgcagcg tgaaggcttc tcgcagaccg cagacgaaat catacataat gcggacctgg
     1441 ccctctatca ctccaagctg agtggtcgaa accgtacgca tgcctactcg gaggatgcat
     1501 atgtggattt ttcgacaaat gagccgagct ctcacgcgga accggttcag gaggaacctg
     1561 agtcaactca gcaggcagcg gaccttggga agtctgaccg gacaatggct gcactggaaa
     1621 gtgatgcgtc ttcagggaca gatgccgcac aagcaacccc gcaggctcac cgtgaaaagc
     1681 ctcaaaaacc cgattacgcg gtggggattt tcatcgggct gctcgccctg atttctgttc
     1741 tgtcgctggc agctgttgtg agctggatgt cgctattgga aacgggcgcc ccctttgatt
     1801 ggcccggtct gcttgttctt tcggttttga ttgtcgtgtc ggaggggttc tcgattgatt
     1861 tgtacttcag gcaaaattct gtatccacgt ccgcgctccc gatcctcgta gcctacctgg
     1921 tctttggccc tatcggcact gtttctgcaa gcctgatact ggccattgcc ttgatgatta
     1981 agtatcggag ccgtttcagt cgatttgtat tcaattttag caatcatctc cttgccggtg
     2041 cttttgccct ggcaatggtg atgctggccg ggggcaactt cattcagttg agtccgatct
     2101 atcaaatcct tttgagcctg gcatctgctg ccatcctgta tctcaccacg accagtatga
     2161 ttgcgtttgg tatgagcctt g
//
LOCUS       DASTJP010000513         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1410809, whole genome shotgun sequence.
ACCESSION   DASTJP010000513 DASTJP010000000
VERSION     DASTJP010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410809"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..439
                     /locus_tag="VFQ23_15045"
     CDS             173..439
                     /locus_tag="VFQ23_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907966.1"
                     /translation="MEEILLLTVEHVVELSEDVLITPFLPVDKTGRWLHQIERVKIVK
                     PDHQTAEKEAEFGIPFDADVYILLIPNTKQEEIPIGSQIWIARF"
     gene            complement(446..>2995)
                     /locus_tag="VFQ23_15050"
     CDS             complement(446..>2995)
                     /locus_tag="VFQ23_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907967.1"
                     /translation="WRHIFAQLFGLDFTTSMSGRKEALVSTLDDDLVERAALLNDILQ
                     VDFPHTELTANLDGRVLAENTRAMLTTILHRATRQTPRLITIEDAHWMDSASLALLMD
                     VSRLVNETFLMLIITTRPLTGSTASEAAQLLRLPYARHIKLEPLPAQETLTLVAYKLG
                     VRELPAEVNALILKKAEGHPFFSEELAYALRDTGILLIENGRSLLAPGINNLETLDLP
                     ETVQGVILSRIDRLTPIQQLTLKVASVIGRVFEYATLKAVYPIEAERTMLRPSLVSLE
                     QLDITPLETPDPELAYIFKHSITRDVAYDLMLFAQRRQLHRSAAEWYEQTHAKDLEPY
                     YGLLAHHYALAEESAKAIQYLEKAGDHALSRGAYVEGVNFFDEAIRISEQNRREVHFA
                     PVQRARWEFQLGEAYMGLGTLQEGRKHFEHAATLLGWASPKRKSALLMSVLMQLGWQV
                     TRLIRGKPFRPARDPSRALDAARVHERLGELYYFAQDRLRLLNSTLHTLNLSQKAPPS
                     PELARAYALACSVATIIRLRGLAKRYEEHALQTAAQLNNLPAQARVLSRTGYYNIGIG
                     NFKRAIQLLDQAAEISERLRDYRQWGESASLRAWTAYLIGDFEDNRARYERVFLMAKQ
                     VGNIQYLNWGQWGQAHGLLRMNRLEESRRALASVIKQIESGEYSGAQVLAFGLMSIVC
                     MHLQDWANMLEYGKRLVRTERRPDRISIADFEGYASVAEVFLTLWQQAANTDILKQIN
                     LPLEECQTYARRAIKSMQRFARLYALAKPRSHDLTGWLEWLDGQQQDAIQTWKKGLRL
                     HQTAEMPYEEARLHHILGKHLPSDDPESQAHREQARQLFIRLNAQLDLNKE"
BASE COUNT          703 a          781 c          795 g          716 t
ORIGIN      
        1 aatctttaaa tgaattagcg aaaatatgga agtaatcgta gggcggattg ccaatccgcc
       61 ttacctcaag agtgagccgt gatacaggta attaaataaa ttaacgaagt tgtggaaata
      121 gaaaaagtat ttatgacctg cccaataaaa gtgttgatat ctagattgca atatggaaga
      181 aatactactg ctaacagttg agcatgttgt tgaattaagt gaggatgtgt taatcactcc
      241 ttttctgcct gttgacaaaa ctggacgctg gcttcatcag atcgagagag tcaaaattgt
      301 gaagcctgac catcaaactg ctgaaaaaga agctgagttt ggaattccct ttgatgctga
      361 cgtatatatt ctcctgatac caaataccaa acaggaagag attcctatcg gttctcaaat
      421 ctggatcgca cgtttttaag ccgaattatt ctttattcaa atccaattgc gcattcaacc
      481 gtataaagag ttgcctggcc tgctcgcgat gagcctgtga ttcggggtcg tcgctgggta
      541 aatgtttgcc caatatatga tgcaggcgcg cctcttcgta gggcatttct gctgtctggt
      601 gcaggcgcag gcccttcttc caggtttgaa tcgcatcctg ttgttgacca tccagccatt
      661 ccagccagcc tgttagatcg tgcgagcgcg gcttggcgag tgcatagagt ctggcaaatc
      721 gctgcatgga tttgattgcg cggcgcgcgt aggtctgaca ctcttcgagc ggaagattga
      781 tttgtttcaa aatatctgtg ttggcagcct gctgccaaag tgtgaggaaa acttcggcaa
      841 cgctggcgta gccttcaaag tccgcgatag aaatacggtc gggtcttcgc tctgtgcgca
      901 cgagccgttt tccatattca agcatattcg cccaatcctg caggtgcata caaacgatgg
      961 acatcagacc gaaagctaac acttgcgcgc cgctgtattc cccgctttct atctgtttga
     1021 tcacggatgc caaagcccgc cgggattctt cgaggcggtt cattcgtaac aagccatgcg
     1081 cctggcccca ctgaccccag ttcaggtact gaatattgcc cacctgcttt gccatcaaaa
     1141 agacgcgctc atagcgggcc cggttatcct cgaagtcgcc gatcagatac gccgtccagg
     1201 cccgcagaga ggcactttct ccccactgtc gatagtcccg caggcgttct gaaatctcag
     1261 ccgcctgatc aagcaattga atggcgcgtt tgaaattgcc aataccaatg ttatagtacc
     1321 ccgtgcgcga aaggacgcgt gcctgcgccg gtaggttgtt caactgcgcg gcggtctgca
     1381 aagcatgttc ttcatatctc ttcgccaggc cgcgcaggcg gataatagta gctacactgc
     1441 aggcgagtgc ataggcgcgt gccagctcag gcgagggtgg tgctttctgt gaaaggttca
     1501 gcgtgtgcaa cgtgctgttc aacagacgca aacgatcctg tgcgaaataa taaagttcac
     1561 ccagccgttc atggacgcgg gcagcatcca gagctcgtga aggatctcgc gccgggcgga
     1621 aaggcttgcc tcgaatcagg cgcgtcacct gccagcccag ctgcatgagc acgctcatca
     1681 gcagcgcgga cttcctcttt ggcgatgccc agcctaacag tgttgctgcg tgctcaaagt
     1741 gttttcggcc ttcctgcagt gttcccagcc ccatgtatgc ttcacccaat tgaaattccc
     1801 atcgagcgcg ctgcaccgga gcgaagtgaa cctcccggcg gttctgttcg gagatgcgaa
     1861 ttgcctcatc aaaaaagttg acgccctcca catacgcacc gcgcgagaga gcgtgatcgc
     1921 ctgctttttc caaatactga atcgcctttg ctgattcctc agccagcgcg taatgatgag
     1981 cgagtaaacc ataatagggt tcgagatctt tcgcatgggt ttgctcatac cattccgccg
     2041 cagagcggtg caactgcctg cgctgagcga agagcatcaa gtcataagcc acgtcacgcg
     2101 tgatactgtg tttgaaaata taggcaagct cagggtctgg tgtttcgaga ggggtgatat
     2161 ccaattgttc caatgacacc agtgagggac gcaacatcgt gcgctccgcc tcgatcgggt
     2221 agaccgcttt caatgtggcg tattcgaaga ctctgccaat tacactcgcc actttgagcg
     2281 tcagctgctg tataggcgtc aggcggtcaa tgcggctgag gattacgcct tgcacagtct
     2341 cgggcaggtc aagggtttcg agattgttga tccccggggc gagcagactt ctaccgttct
     2401 caattaagag gattcctgtg tcgcgcagtg cataagccag ttcttcgctg aagaacggat
     2461 gcccctcggc ctttttcagg atcaacgcgt tcacctccgc cggcaattcg cgcacaccca
     2521 acttatatgc gaccagtgtg agcgtctcct gtgcaggcaa aggctccagc ttgatatggc
     2581 gggcgtaggg caacctcagt aactgtgctg cctccgaggc ggtcgacccg gtcagcgggc
     2641 gagtagtgat gatgagcatt aggaacgttt cgttcaccag acgcgaaaca tccatcagta
     2701 acgccaggga tgcactatcc atccagtgcg cgtcctcaat cgtgattaag cgcggagttt
     2761 gtcgcgtggc gcggtgaaga atggtcgtga gcatagcccg agtgttttcc gcaaggacgc
     2821 ggccatccag attggcagtc agctcggtgt gcggaaaatc cacctgcagg atgtcgttca
     2881 ggagagcggc gcgctcaacg agatcgtcat ccaatgtaga gacaagcgcc tctttgcgtc
     2941 cggacatgga tgtggtgaaa tccagcccaa aaagctgcgc aaagatatga cgcca
//
LOCUS       DASTJP010000514        10409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1069388, whole genome shotgun sequence.
ACCESSION   DASTJP010000514 DASTJP010000000
VERSION     DASTJP010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10409
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069388"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="VFQ23_15055"
     CDS             complement(<1..1949)
                     /locus_tag="VFQ23_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9907968.1"
                     /translation="MTQADIILTNAFILTMDEKLTQYSPGAVAVKGDSILAVGSENEI
                     KKEYSATETIDCSGKVLMPGLINAHTHAPMTLLRGLADDLRLDVWLMGYMMPVEREFV
                     SPEFVQLGTLLACAEQIRSGVTTFNDMYYFEEDVAKAAAQAGMRAVCGQTVMKFPAPD
                     AASYEDSLAMAREFIQRWKDHPLIVPAVAPHAPYTCTAEILRATAELAREFDVPLHTH
                     LAETAFEVENMRSENGIPVIPYVKKQGLLEAKVIAAHCVHIDVGEIRTLMHAGAGVSH
                     NPSSNLKLASGFAPVMKMLETGINVGIGTDGPASNNDLDMFEEVRLAAFVAKAVTNDP
                     TSLPASQALLMATRLGAQALHIGHLTGSLEAGKRADLILVDTSPLHNSPSFKRAPDTA
                     YAQIVYASKSTDVSDVMVNGKWLMRDRQLLTVKEDELLTQAAEFAKKIDSFLIAREQS
                     VLSKLIALGGSMEQESFEVQVKVKITNPEPILQAMKRPEIEILRQRHYSEHDIYFFFD
                     DPGQGRLRYREDDFIDDAKGIVTNVRARLTLIGQKREGQFANEVLLSRSRFIAPASQS
                     PRFYREYFKPKSEVQIDKDRLRWHIKYKDTEFFVNLDRVTEPELGYFLEVKSRTWSRK
                     DADNKAHLANELLSLLEASGGETVT"
     gene            complement(1987..2955)
                     /locus_tag="VFQ23_15060"
     CDS             complement(1987..2955)
                     /locus_tag="VFQ23_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HET9907969.1"
                     /translation="MSRQRKRSHTWVYVLWGFNGIVIFGVLSAFAFYVSNQRPAPADD
                     GVQTMQSNATQTVPPTSYFLPTLTPNPNYTPPALETSTPFVIENGPRPTIIGFSLAGR
                     PIEVYTFGAGEREYLIVAGIHGGYEWNTIALANELITHINENPEAIATDATLYIIRNM
                     NPDGEARAHGVDGRVNNNGVDLNRNFPSENWTAEWDRDGCWIYRPTTGGLYGGSEPET
                     RTVMSFIKSKKIVALISYHSAALGVFPGGVPWEEPSIELAKQLSKATRYLYPPIDTGC
                     EYTGTLADWAVENGVGAAVDMELRNHSETDFDQNLRALKVFLSYIP"
     gene            complement(2959..4092)
                     /locus_tag="VFQ23_15065"
     CDS             complement(2959..4092)
                     /locus_tag="VFQ23_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET9907970.1"
                     /translation="MPSAEIITIGTEILLGEIVDTNTRHIARTLRGMGVDLYRTITIG
                     DNTERIAEAIHNSMNRAEIVITTGGLGPTVDDPTREAVARAAGVELEFREDLWQQVVA
                     IISRYGRKPSENQKRQAYVPKGSIGITNPVGTAPCFIVENERNAVISLPGVPNEMEYI
                     LHESIIPYLQKRFNLDQIIKIRILHCAGLGEGLIDEKIDDLEKLSNPTVGLAAHTGVV
                     DIRIAAKAKSETEADDMIASIEKDIRARLGDVVFGADEDKLETITLNEVAKRGWTVTA
                     IESGLDGLLARKIPHTTSFSELNPAALMVALHAARIEANADAALGVALYPEERAAEIA
                     LITPKGEKTHHITYGGPPRSLPRWAVNLALDWLRRRAMDPGGE"
     gene            complement(4195..5184)
                     /locus_tag="VFQ23_15070"
     CDS             complement(4195..5184)
                     /locus_tag="VFQ23_15070"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907971.1"
                     /translation="MLILNANEVRQALPMKQVIESMKEAYASLSGGRAVVPLRTRVQI
                     AKHEAVSLFMPAYMTSDMGEALAIKVVSLFPNNPPRGLAYIQAAVLVLEADTGRAIAL
                     LEGSTLTAIRTGAGSGAAIDILAREDSKRVAIFGAGAQGRTQLEAACNARTIETVFVF
                     NPTLEKANAFAKELAGHNSIPQDVRVAQSPREAIENADIICTATTSSKPVFDDIYVRP
                     GAHISAVGAYTPEMQELPVETVARARIVVDSYATVMEEAGDIVQAIRAGAIQESDIHA
                     ELGEIILGKKTGRESEDEITFFKSVGNAIQDAVAARLALQNARTMGLGTEVDF"
     gene            complement(5290..6003)
                     /locus_tag="VFQ23_15075"
     CDS             complement(5290..6003)
                     /locus_tag="VFQ23_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907972.1"
                     /translation="MPMLELENIHTYYGNIHALKGISLTVEKGEIVTLIGANGAGKST
                     TLRTITGLLKPRNGSVKLDGDDLGRYKAHDIVYKGIAMVPEGRGIFARLTVTENLDMG
                     AYSRTSKAEYDQDVERIFALFPRLKERRNQVAGTLSGGEQQMLATGRALMAHPTLLLM
                     DEPSMGLAPLLVELIFSTIEQINKEGMTILLVEQNALMALSVANRGYVLQSGSIVLQD
                     SAANLKKDPTVQKAYLGME"
     gene            complement(6024..6809)
                     /locus_tag="VFQ23_15080"
     CDS             complement(6024..6809)
                     /locus_tag="VFQ23_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9907973.1"
                     /translation="MSNILNAESVTKRCGGLTAVGKLSIEIPEKSIYSVIGPNGAGKT
                     TFFNCITGFYQPEEGDIIFDGRSILGLSSDQISQRGISRTYQNIRLFSNLTAIENVLV
                     GQHALLKSTLFGPIFGLPSTKREEAEAVTEAKRLLAFVSLARKGDLLAKNLPYGDQRR
                     LEIARALASKPKLLLLDEPTAGMNPNETEDMTKFILRLRDELGITILLIEHDMRVVMG
                     ISEKITVLDYGEKIAEGTPKDIQGNERVIEAYLGRGSAAQITA"
     gene            complement(6837..8540)
                     /locus_tag="VFQ23_15085"
     CDS             complement(6837..8540)
                     /locus_tag="VFQ23_15085"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907974.1"
                     /translation="MENESKTSWWLQALQKGAIGGVVTVLLSLIGMVASFHERDIIAG
                     QITMGQMLLLLIVVAFAYYSSRGTENTTLANRLISGALTGFVTASFIVGLVLLGKAVN
                     LREVFVNASPNLYKILTFDMENVGTAFVYLLLAGTLIGFLTALVHLIPSLWRNAIIGG
                     LTIALLIALLQDLIRITLSPFPAVTEALAWMFGSRGQEGLSVVGTIVVFLVVAAIRYI
                     SSIQGDNVRGRLQSLPKSTQQGLRWFSWIVLLLLLLIWPRVAGTYLSEVTNTVGLYIL
                     MGLGLNIVVGYAGLLDLGYVAFFALGAYAMAVLTTTGGEISANFQWTFWQAFPLAVVI
                     AVIAGVVLGIPVLKIRGDYLAIITLGFGEIIRIMFLSDLLKPYLGGSFGIVEIAAPTF
                     GDLTLNTPQTLYYVILGGCLLALFVGYRLRDSRMGRAWKAIREDEDVAQAMGINLTAT
                     KLLAFATGAAFGGLSGAIFAAKLSTVYPHSFVLLVSINVLALIIVGGMGSLPGVIVGA
                     LLLVGLPELLREFSEFRLLVYGALLIAMMLNKPEGFIPEAVHRRELHEEAEDNVQPAA
                     A"
     gene            complement(8620..9681)
                     /locus_tag="VFQ23_15090"
     CDS             complement(8620..9681)
                     /locus_tag="VFQ23_15090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9907975.1"
                     /translation="MNQRFWNRINFTDIALGVIGISLVGLIVVGSFKTLSEGNYTLRQ
                     WLDFVVFGVAQGSIYALIALGYTLVYGILRMINFAHSEVFMSGAYTAYFAASAMAKSG
                     FLRENPFVAILILFVIAMLTSVAIGVLVERIAYRPLRRAPRLVPLITAIGASFFLQYT
                     FKGLYGDQFKAYPEIAALKGNIVLGDLNITKIQVLVIVSAALLMLGLYLYVQYTKVGK
                     AMRAVSEDKDVAALMGIDVDAIIVRTFVLGAALAGAAGILYALTFRQVNYLMGFIPGI
                     KAFTAAVLGGIGNIPGAMLGGLFLGIFESVGPILFLDGLNVPAPYQLKDVIAFTMLVL
                     VLLFRPSGILGERLSRSKA"
     gene            complement(9761..>10409)
                     /locus_tag="VFQ23_15095"
     CDS             complement(9761..>10409)
                     /locus_tag="VFQ23_15095"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET9907976.1"
                     /translation="FTMGSDWKDPTADEDEKPSHNVLLDSFWMDRTEVTNAMYLRCVS
                     AGACTPPARSSYYEKPEYAEHPAIGISWPQAQDYCTWVERRLPTEAEWEKAARGTDER
                     IYPWGNDLPATQHANFNHNLNETSDVGNFPEGASPYGVLDMAGNAWEWVADGYQPDFY
                     SQSPEKNPITDSPVNRRVLRGGNWDSNADGIRVTNRFWAFPGRNDTDGFRCAQSD"
BASE COUNT         2540 a         2722 c         2536 g         2611 t
ORIGIN      
        1 ttcgtcacag tttctccacc agaggcttca agaagggaga gcaactcatt cgccaggtgc
       61 gctttgttat cagcatcttt acggctccat gtgcggctct tgacctccag gaagtacccc
      121 agttcaggtt cagtgacgcg atccagattc acaaaaaatt ccgtgtcctt atatttaata
      181 tgccagcgaa gcctatcctt atcaatctgc acttcagatt tgggtttgaa gtattcacga
      241 taaaacctgg gggattggga ggcaggagcg atgaagcgac tgcgcgaaag caatacctca
      301 tttgcaaact gaccttcccg tttctgaccg atcaacgtca ggcgggcgcg gacgttggtg
      361 acgatcccct tcgcatcatc aataaaatca tcttcacgat agcggaggcg tccttgccct
      421 gggtcatcga agaagaagta aatgtcatgt tcgctatagt gccgctggcg caggatttcg
      481 atctcaggac gcttcatggc ctggaggatg ggctccggat ttgttatctt gaccttgacc
      541 tgcacctcga acgactcctg ttccatcgaa ccgccgagcg cgatcaactt tgacagcaca
      601 gactgttcac gtgcgatcag gaaggaatca attttcttcg caaactctgc ggcctgggtg
      661 aggagctcat cttccttgac cgtaagcaat tggcgatcac gcatcaacca ctttccgttg
      721 accatcacat cacttacatc cgtggacttg ctggcataca caatctgtgc ataggctgtg
      781 tcgggtgcgc gtttgaagga tggcgagttg tgaagaggag aggtatccac gaggatcagg
      841 tctgcgcgtt tccctgcttc aagggaacca gtgagatgtc cgatatgaag tgcctgcgca
      901 ccgagacgcg tcgccataag caatgcctgt gaagccggca gagaggtggg gtcatttgtc
      961 actgccttgg cgacaaaggc cgccaagcgc acttcttcaa acatatccag gtcgttattc
     1021 gatgccggtc catctgtgcc aattccaaca ttgatcccgg tttcaagcat cttcatcaca
     1081 ggggcgaatc ccgaggcaag cttgaggttc gacgatgggt tatgagacac acccgcgccg
     1141 gcgtgcatca gcgtgcgtat ctcgccgaca tcaatgtgca cacagtgcgc cgcaataacc
     1201 tttgcctcga gtagaccctg ctttttcaca tacggaatga ctgggatgcc attttcacta
     1261 cgcatattct cgacttcaaa cgccgtttct gccaggtgag tgtgcagggg aacatcaaat
     1321 tcccgggcaa gttcagctgt tgcgcgcagg atctctgcag tacaggtata cggtgcgtgg
     1381 ggcgccaccg cagggacgat cagcggatgg tccttccagc gctgaatgaa ctcacgcgcc
     1441 attgccaacg aatcttcata ggatgctgcg tctggcgcag ggaatttcat taccgtttgc
     1501 ccgcaaactg cgcgcatgcc agcctgcgcg gctgctttgg ccacatcttc ctcaaagtaa
     1561 tacatgtcgt tgaaagtggt gacgccgcta cgaatttgct ctgcgcaggc gagcagcgtc
     1621 ccgagttgaa caaactctgg cgagacaaat tcccgctcga ccggcatcat gtatcccatc
     1681 aaccagacgt cgaggcgcaa gtcatcggcc agtccgcgta acagagtcat gggcgcatgc
     1741 gtgtgtgcgt tgatcaggcc aggcatcaga accttgcccg agcaatcaat cgtctctgtt
     1801 gccgaatatt cttttttgat ctcgttttca gatccaacgg cgaggatgct atcgcccttg
     1861 actgccacgg caccgggaga atattgtgtg agcttttcat ccatggtgag gatgaaggcg
     1921 ttggtgagga tgatatctgc ttgagtcatg gtcttatcct ttgggtactt ttattgacat
     1981 gagcattcac ggaatatagc ttaaaaacac cttgagtgct ctcagattct gatcgaaatc
     2041 ggtttcacta tgattgcgca attccatatc caccgccgcg cccacgccgt tctctacagc
     2101 ccagtccgcg agtgtacccg tatattcaca gcctgtatca atgggtgggt ataaatatct
     2161 cgttgcttta gacagttgct ttgcaagttc tatggagggt tcctcccacg gcacaccacc
     2221 cggaaataca cccagtgctg cgctgtggta gctgatcagc gcaactatct ttttcgactt
     2281 aataaaactc attaccgttc gggtttctgg ttctgagccg ccatacaggc cgccagttgt
     2341 cggacgatag atccaacatc catcgcgatc ccattcagcg gtccagtttt cggaagggaa
     2401 gttgcggttc agatccacac cgttgttgtt aactcttcca tccaccccat gtgcccgcgc
     2461 ttcgccatcc ggattcatat tgcgaataat gtagagcgtt gcatctgttg cgatggcttc
     2521 cgggttttca ttgatgtgcg taatcagttc atttgcaaga gcgattgtgt tccattcata
     2581 gccaccatgg atacctgcta cgatgagata ttctctctca ccagcaccaa atgtatacac
     2641 ctcaatcggg cgaccggcca gtgaaaagcc gatgatcgtt gggcgaggtc cgttttcaat
     2701 aacaaatgga gtggaggttt ccagtgctgg tggtgtgtaa tttggattgg gagtaagcgt
     2761 tggtagaaaa taacttgtag gcggcacagt ttgtgtagca ttcgattgca ttgtctgaac
     2821 accatcgtcc gctggtgcgg gccgctgatt ggatacataa aacgcaaagg cgctcaacac
     2881 gccaaagata acgatcccat taaatcccca aaggacatag acccaggtgt gggatctttt
     2941 cctttgccgc gacataggtt attcaccacc tgggtccatg gcgcggcgcc ttaaccaatc
     3001 aagggcaagg ttaacagccc agcgcggcaa actacgcggc ggtccgccat acgtgatgtg
     3061 atgagtcttt tcgcctttgg gggtgatcag tgcaatctct gccgcgcgtt cctcaggata
     3121 tagcgccaca cccagggccg catctgcatt ggcttcgata cgagcggcat gcagtgccac
     3181 catcagtgca gccggattaa gctccgagaa agacgtggta tgaggaattt tgcgcgctaa
     3241 caagccgtcc agaccagatt caatggcagt cacggtccag ccacgtttcg caacttcgtt
     3301 cagagtgatc gtctccagtt tgtcttcgtc cgcgccaaag acgacgtctc ccaaccgtgc
     3361 ccgaatgtct ttctcaatac ttgcaatcat atcgtcggct tcagtttcac tttttgcctt
     3421 ggctgcaatg cgaatatcga ccacgccggt gtgcgcagcg agcccgactg tcggattgct
     3481 caatttttca agatcgtcaa tcttttcatc aataaggcct tcaccaagcc ccgcgcaatg
     3541 caagatgcgt atcttgatga tttgatctaa attgaatcgc ttttgcaaat acggaatgat
     3601 cgattcgtgc agaatgtatt ccatctcgtt ggggacgccc gggagggata tgacggcatt
     3661 ccgttcattt tcaacgataa aacagggcgc cgtgccaacg ggattcgtga tcccaattga
     3721 acctttcgga acatatgcct gccgtttttg attctcggag ggtttccgtc cgtatcgcga
     3781 aatgattgca acaacctgct gccaaaggtc ttcgcggaat tcgagttcaa ccccggccgc
     3841 ccgggcgaca gcctcccgtg tgggatcatc cacagtggga cccagcccgc cggtcgtaat
     3901 gacaatttcg gcgcggttca tcgaattgtg tatcgcttcg gcgatacgtt ccgtgttatc
     3961 accgatggtg atcgtgcgat acaaatccac gcccatgcct cgcaaggtgc gcgcgatgtg
     4021 acgcgtattg gtatctacaa tttcccccag taaaatctcg gttccgattg ttatgatctc
     4081 tgcggatggc ataaattatt gcacgtaagt gtatcatgga tgctcgcgct gcgattgtgt
     4141 tctgacccct cgcgagtgct gtccttactt ccttcaacac gatatgtact taaattagaa
     4201 atccacttct gtgccaagcc ccatggtacg cgcgttttga agtgcgagtc gggctgcgac
     4261 tgcatcctga attgcgttcc caactgattt gaagaaggtg atctcatctt ctgattccct
     4321 acccgtcttt ttacccaaaa tgatctcgcc cagttcagca tggatatcag attcttgaat
     4381 cgcacccgca cggatcgcct gcacgatatc cccggcttcc tccatgacag tcgcgtacga
     4441 atcgacaaca atacgggcgc gtgctacggt ttcaaccggt agctcctgca tctcaggcgt
     4501 gtacgcgccg accgcagaaa tatgcgcgcc gggtcttaca taaatatcat cgaacacagg
     4561 tttggatgaa gtggtggctg tgcaaataat atctgcgttt tcaatggctt ctcttggact
     4621 ttgtgcaact cgaacgtcct ggggaatgga attatgccct gccagctctt tggcaaaggc
     4681 attcgctttt tccagcgtcg gattgaaaac gaaaactgtt tcaatggttc gtgcattgca
     4741 ggcagcctcg agctgagttc gtccctgggc acccgcgccg aaaatcgcta cccgcttact
     4801 atcttctcgc gcgaggatat cgattgcagc tccgctacct gcaccggtac ggatggctgt
     4861 cagggtgcta ccttccagca aagcgatggc gcggccggtg tcagcttcaa gcaccaggac
     4921 ggctgcctgt atgtaagcca gcccgcgcgg gggattattt ggaaataatg aaacaacttt
     4981 gattgccagt gcttcgccca tgtctgacgt catataagct ggcatgaaga gactaacagc
     5041 ctcgtgtttt gcgatctgga cgcgcgtccg aagcggcaca acagccctcc ctcccgagag
     5101 agatgcatag gcctctttca tggactcgat gacctgtttc atcgggaggg cctgcctgac
     5161 ttcatttgca ttgagtatta gcattctatt tccttataat taccgtattt gctgcgacac
     5221 ataatggtaa taaaaagcgc agaggttctg cgttcgcctc tgcgcttcct atgtatgatt
     5281 cggattcttt tattccattc ccagatacgc cttctggacg gtcggatctt tcttcaaatt
     5341 ggctgcggaa tcctgtagca caattgagcc tgattgtaga acataaccac ggtttgcgac
     5401 tgaaagcgcc attaaggcat tttgttccac cagaagaatc gtcatgcctt ccttgttgat
     5461 ttgctcaatg gtggaaaata tgagttccac aagtaaaggt gccagcccca tggagggttc
     5521 atccatcaga agcagagtgg ggtgtgccat aagcgcgcgt cccgtggcaa gcatctgttg
     5581 ctcaccaccc gaaagagttc cagccacttg attgcggcgc tctttgaggc gagggaacaa
     5641 tgcaaagatg cgttcaacat cctggtcata ctccgctttg gaggttcggc tgtaggcacc
     5701 catatccagg ttttcagtga cggtcaggcg ggcgaaaatt ccgcgccctt ctggcaccat
     5761 agctatgcct ttgtatacaa tgtcatgcgc cttgtatctc ccaagatcat cgccatcaag
     5821 cttaacgctt ccattgcgtg gcttcagcag gccggtaatc gtgcgcagag tagtcgattt
     5881 gcctgcgccg ttggcaccaa ttagtgtaac gatttcgccc ttttctacgg taagtgaaat
     5941 gcctttcaag gcgtggatgt ttccataata tgtgtgtatg ttttcaagtt caagcatggg
     6001 catggctgta ttctcctcga ggtttaggca gtgatctgcg ctgcactgcc gcggcccaga
     6061 taggcttcaa tgacgcgctc attgccctgg atatcttttg gggttccttc ggcgatcttt
     6121 tcaccgtaat ccagaactgt tattttttct gaaattccca tcaccacgcg catatcatgc
     6181 tcgatcagaa ggatcgtgat tccgagttca tcgcggagcc gcaggatgaa tttggtcatg
     6241 tcctcggttt cgtttggatt cattcctgcg gttggctcat cgagcagcag taactttggc
     6301 ttgctggcta aggcacgcgc gatttccagc cttctctgat cgccatacgg cagattcttg
     6361 gcaagcaagt ctcctttgcg tgccaacgaa acaaaagcca gaagacgttt ggcttccgtc
     6421 actgcctctg cctcttcacg ctttgtgcta ggcaagccaa aaatcggacc gaaaagggtg
     6481 gattttagaa gagcatgctg acccacgaga acattttcga tggcggtcag atttgaaaag
     6541 agacgtatgt tttggtacgt tctcgagatg cctcgctgac tgatttggtc cgatgacagg
     6601 cccaagatcg aacggccgtc aaagatgatg tctccttctt caggctgata gaaaccggtg
     6661 atacagttga agaaggtggt tttcccggca ccatttggcc caatgacgct atagattgat
     6721 ttttctggaa tttctatgct gagcttaccg acagccgtaa ggccaccaca cctcttggta
     6781 acactttctg cgttgagtat gttagacatg agtaatatcc ttatatggaa ttacgcctag
     6841 gcggcggccg gttgcacgtt atcttctgct tcttcatgta attcgcgccg gtgaactgcc
     6901 tcggggataa agccctccgg tttgttgagc atcatcgcga tcagaagcgc accgtacacc
     6961 agaagtcgga attcggaaaa ttctcttaag agttcgggga gcccaaccaa cagcagtgcg
     7021 ccgacgatca cgccggggag actgcccatt ccaccgacga taataagcgc aagcacattg
     7081 atcgaaacca acaacacgaa actgtgcgga tagacggttg aaagtttggc ggcaaagatt
     7141 gcgccactca atcctccaaa agcggcgcct gtggcgaagg caagcagctt cgttgctgta
     7201 aggttgattc ccatggcctg cgcaacatct tcatcctcgc gaatggcctt ccaggcgcgc
     7261 cccatccgcg agtcgcgcaa gcgataacca acaaaaagcg ccagcaggca gcctccgagg
     7321 attacataat agagcgtctg tggcgtattt aatgttaaat ctccgaatgt gggtgcggca
     7381 atttcaacta tcccgaatga tccgccaaga tatggcttga gcaaatcgga aaggaacatg
     7441 atgcggataa tttcgccgaa tccaagtgtg ataatggcaa gataatcacc acggatctta
     7501 agcaccggga tccccagcac aacccctgca atcacagcga taacgactgc tagcgggaat
     7561 gcctgccaaa atgtccattg aaaattcgca ctaatctctc cgcctgttgt tgtcagcact
     7621 gccatggcgt aggcgcccag agcaaagaat gccacataac caaggtccag caggcctgca
     7681 tatccaacta caatattcaa accaagcccc atcaggatgt acaaaccaac ggtgtttgtt
     7741 acttcactca agtatgttcc ggctacgcgg ggccagatca acaacaagag caaaagcaca
     7801 atccagctaa accagcgtag tccttgttgt gtgctttttg gcaacgattg aagccgcccg
     7861 cgtacattat ccccttggat agaggagata tatcttatgg cggcgaccac caggaagaca
     7921 acaattgttc cgactacaga caagccttcc tgtccacgcg aaccaaacat ccaggcaagt
     7981 gcttctgtga ccgccgggaa tggagatagc gttatccgaa tcaagtcctg aaggagagca
     8041 atcaacaaag cgatggtcaa tccgccaata atggcgtttc tccacaagga agggataagg
     8101 tgaactagtg cagtgagaaa accaatgagc gtgccagcca gcaacaaata cacaaatgct
     8161 gtgcctacat tctccatatc aaaggtgaga attttataga gatttggcga agcattgacg
     8221 aaaacctcac gcaaattaac agcttttccc aacaagacaa gccctacgat aaaactggct
     8281 gtgacaaagc cggtcagggc cccgctgatc aaacggttag caagcgttgt gttttccgtg
     8341 cctcgagagg aatagtaggc aaatgccaca acgatgagca gtagcaacat ctgccccatc
     8401 gtgatctgac ctgcaataat gtccctctca tggaaggatg caaccattcc aatcaggctc
     8461 aagaggacag tgaccacacc gccaatcgcg cctttttgta gagcctgtaa ccaccagctt
     8521 gtttttgatt cgttttccat ggttagcctc ttcgccgtgg tagcgacttt agtcgcactt
     8581 taagaaacga ctgaagtcat tactacactt atattttagt tacgctttac ttcgagaaag
     8641 ccgttcgccc aggatgccgc ttggacggaa gagcaagacc agaacaagca tcgtaaatgc
     8701 aatcacatcc ttgagttgat agggagcagg gacgttcagt ccgtcaagga agagaatggg
     8761 tcccacagat tcaaagatgc cgaggaagag tcctccaagc atcgctccgg gaatgttgcc
     8821 aatccccccc aataccgcag cggtaaaggc cttaatgccc ggaataaaac ccatcagata
     8881 gtttacctgg cgaaaggtga gtgcatacag gattccggca gcacccgcca gcgccgcgcc
     8941 aagcacaaaa gtgcgtacga tgattgcgtc cacatcgata cccatcagtg ctgccacatc
     9001 tttatcttcc gataccgcac gcattgcctt gccaactttt gtatattgaa cataaagata
     9061 aaggcccaac atcagcagtg ccgccgagac gatgactaac acctgaatct ttgtaatatt
     9121 caaatcgccg agaacgatat ttccctttag tgcagcgatt tctgggtacg ccttaaattg
     9181 atcaccatat agacctttga aggtatattg gagaaaaaac gacgctccaa tggctgtaat
     9241 aagaggcaca agccttggag cgcgtcgcaa tggacggtac gcgattcgtt cgaccaatac
     9301 gccaatcgcg acagatgtga gcattgcaat tacgaataag ataagaatgg caacaaaagg
     9361 attttcacgt aagaaacccg attttgccat ggcactcgct gcaaagtacg ctgtataagc
     9421 accactcata aacacttcgc tatgtgcaaa attgatcatt cggaggatac cgtatacaag
     9481 ggtgtacccc agcgcgatta gtgcataaat actcccctgc gcaaccccaa agacgacaaa
     9541 atcaagccat tgacgaagag tgtaatttcc ctcactaaga gttttgaagg agcctacgac
     9601 aatcaaaccg accagcgata taccgatgac gccaagagca atatctgtga aattgattcg
     9661 attccaaaat ctttgattca tgattggctc ccgtcaggca cgaaatatga attgctttgc
     9721 aaatttgtta aggcgctatt atattctatt tcctgtattt ctagtcagat tgggcacagc
     9781 ggaacccatc tgtatcattt cgtccgggga aggcccaaaa gcgattcgtg actctgatgc
     9841 catctgcatt gctgtcccaa ttcccaccac gtaagacgcg acgattaacc ggcgaatctg
     9901 tgatcggatt tttttctggt gattgtgaat agaaatctgg ctgatagcca tctgcaaccc
     9961 attcccaggc gttgcctgcc atatccagca caccatatgg actcgcgccc tctggaaagt
    10021 ttccaacatc acttgtctca tttaggttgt gattaaagtt tgcatgttgt gtggctggta
    10081 aatcattgcc ccatggataa attctttcat ccgttcctcg cgcagctttt tcccattcag
    10141 cttcggtagg caatcgtcgt tccacccaag tgcaatagtc ctgtgcctgc ggccaactga
    10201 taccaattgc gggatgttct gcgtattctg gtttctcgta ataagaggat cgagctggag
    10261 gagtacatgc accagcggaa acacaacgaa gatacattgc atttgtcacc tcggtgcgat
    10321 ccatccaaaa tgaatccaat aatacgttgt gcgacggttt ttcgtcttcg tccgctgttg
    10381 gatccttcca atcgctgccc atggtgaac
//
LOCUS       DASTJP010000515         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_732427, whole genome shotgun sequence.
ACCESSION   DASTJP010000515 DASTJP010000000
VERSION     DASTJP010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732427"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFQ23_15100"
     CDS             complement(<1..276)
                     /locus_tag="VFQ23_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9907977.1"
                     /translation="MSKSRTQQMVDRLRELQASSPDIEASAVVSVDGLSIASALPQGV
                     EEDRVSAMSAAMLSLGERIASELGRGSLEQVYIKGEKGYVVLMSVGEE"
     gene            614..687
                     /locus_tag="VFQ23_15105"
     tRNA            614..687
                     /locus_tag="VFQ23_15105"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:648..650,aa:Asp,seq:gtc)
     gene            1081..1299
                     /locus_tag="VFQ23_15110"
     CDS             1081..1299
                     /locus_tag="VFQ23_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907978.1"
                     /translation="MQVGNIRLYKDRWKAVEEIERQELRAMTFEDHWRKLNAIVRFAL
                     GTGMEIKRDNSEMEVFSRWSKLKSTYEA"
     gene            1289..1795
                     /locus_tag="VFQ23_15115"
     CDS             1289..1795
                     /locus_tag="VFQ23_15115"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HET9907979.1"
                     /translation="MKLDGSLEPFRAAIMSLQRLLETFNERGVIIGGIAVGFLGKPRF
                     TADIDAVFLLSIKDIPQFIDRAQTEDIIPRIPDAADFARKNRVLLLKHSPTDTDIDVS
                     LGILPFEEEMVERAKTQSFGGLSVRLPTPEDLIIMKAIAHRPKDLDDIRTIADKYDQE
                     RYRCVFQN"
     gene            complement(1902..>2143)
                     /locus_tag="VFQ23_15120"
     CDS             complement(1902..>2143)
                     /locus_tag="VFQ23_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907980.1"
                     /translation="TEAAALGAGILAATAAGCFTNAREAAQEMTHILPQPTEPNASVH
                     SFYNRLFEEVYRHLFPAMQLYLDRLASLSNSNQSI"
BASE COUNT          569 a          492 c          516 g          566 t
ORIGIN      
        1 ctcttccccc accgacatca ggaccacata tcctttttcc cctttgatat aaacctgttc
       61 aagtgaaccg cgtcccaatt ctgatgcaat cctctcgcca agtgacagca ttgcggcaga
      121 catcgctgaa acacgatcct cttccacgcc ttgaggcaaa gcagaggcga tgcttaagcc
      181 gtccacgctg accactgcag atgcttcaat atcaggtgaa gaagcctgca gctcgcgtaa
      241 acggtctacc atttgttgcg tgcgtgattt tgacaagaca accctcctac atggagttga
      301 tgccagacga tgcttcgtcg ggtgggcttg aagtgaagtg cggataattt agcatagtgg
      361 actgaatgtg tcaagccagc ttgattatac gctcaaattt aactaacaga aatgcaaatt
      421 agggatccca tgacgctttc cgtacccggc taaacaccaa ccgtggatac gtttctctta
      481 aacgagatca ttgaagcggg agccacattt gttatgcatt tgagcgcccg attttcagga
      541 cactttttta cgtgtgtgta ttgacactta tacacacgca tggtaaactc ctgcccgctt
      601 gtttggaaga actggtcccg tggtgtagcg gccaaacatg cggccctgtc aaggccgaga
      661 tcgcgggttc gaatcccgtc gggaccggaa ttattgacca cttgatgaat tttcatcaag
      721 tggttttctt ttgccgactg gattggcaga atcctgtaag ccagcgagtg atcacatggt
      781 tagttttgat cttccattta ttcactcact attttaacgt tttcaaaaga tggcgtatct
      841 ttgtggcgta aggactgaga caccgcaggg atagtgaggt tatgttgtat tcataggggc
      901 aatccttttc ggcgaaaaag tgaatatcac cttggatcat acaacctttc aaaaaggggt
      961 ggtttctcaa ccgacgcgaa gagtggatgt agcaggctca cacaattcat ctggtgtgat
     1021 gggtattggg aggcagtcca acaaatgtgt ccagaatttg accgtgctat aattgtcatt
     1081 atgcaagttg gcaatatccg cctttacaaa gaccgctgga aagccgtgga agaaatcgaa
     1141 cggcaggaac tgcgcgccat gaccttcgag gatcattggc gaaaactcaa cgcgatagtt
     1201 cgttttgccc tagggacagg tatggaaatt aaaagggata acagcgaaat ggaagtattc
     1261 tcgcgttggt ccaaattaaa atctacttat gaagcttgat ggaagccttg agccatttcg
     1321 agctgcaatc atgtctctgc agcgccttct ggaaacgttc aacgaacgcg gggtgattat
     1381 tgggggcatt gctgttggtt ttttaggtaa gccacggttc acagcagata ttgatgctgt
     1441 tttcttacta tcaatcaaag atatccctca atttatcgac cgcgctcaaa ccgaagatat
     1501 cattcctcgt atcccggatg cagccgattt tgcccggaag aaccgagttc tgttactcaa
     1561 acatagcccc acagatactg atattgatgt ttctttgggt attcttccgt ttgaagaaga
     1621 gatggtcgaa agagcaaaga cacaatcctt cggaggcttg tcggtccgcc tccccactcc
     1681 tgaagactta attattatga aagcgatcgc tcatcgtcct aaagacctgg atgacatccg
     1741 aacgattgct gataaatatg atcaagaaag atatcgctgc gtcttccaaa actagagatt
     1801 tttgtatagc gggattacct atgtggcttg gagtaatata aaactgtggt cggtaaggaa
     1861 cacgtcagtt aaatcctgcc gcgtaacatc aattgctgga gctaaatcga ctgattcgaa
     1921 tttgatagag aagccaatcg gtccaaataa agctgcatgg cagggaatag gtggcgatac
     1981 acttcctcga acaaacggtt gtaaaaagag tggaccgatg cattcggctc agtcggctgt
     2041 gggagaatat gcgtcatttc ttgagcagcc tcgcgagcgt ttgtaaaaca gcccgccgca
     2101 gtcgctgcca gaatgcctgc tccgagcgcc gcagcctctg tca
//
LOCUS       DASTJP010000516         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1338457, whole genome shotgun sequence.
ACCESSION   DASTJP010000516 DASTJP010000000
VERSION     DASTJP010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338457"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /gene="hisF"
                     /locus_tag="VFQ23_15125"
     CDS             complement(<1..746)
                     /gene="hisF"
                     /locus_tag="VFQ23_15125"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET9907981.1"
                     /translation="MLAKRIIPCLDIKDGRVVKGVNFVNLRDAGDPVEQARLYDAQGA
                     DELVFLDISATHEGRRATLELVERVADSVFMPLTVGGGIREVDDMRNLLLAGADKVSI
                     NSAAVKRPELLSDGAARFGAQCIVLAIDARKKESGWEVFVNGGRIPTGLDAIEWAIRG
                     VELGAGEILLTSMDTDGTLAGYNLELTRAISEVVTVPVIASGGAGSPSHFAQVLTDGK
                     ADAALAASLFHDGKLRIPELKAELQRQGLPV"
     gene            complement(736..1482)
                     /gene="hisA"
                     /locus_tag="VFQ23_15130"
     CDS             complement(736..1482)
                     /gene="hisA"
                     /locus_tag="VFQ23_15130"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HET9907982.1"
                     /translation="MKSFTIFPAIDLRNGKVVRLKEGDPARMTSYSDDPSEMARRWLS
                     MGAQWLHVVNLDSAFGERDSENQTALKSILVTAQEFNAQVQFGGGLRSVVSIEQTLNL
                     GVERAVLGTVAVEHPTIVEEALARFGAKRIAVGIDARDGIVRTRGWKEDGGTSANDLA
                     IQMRTLGLVTVIYTDIRRDGLGSGLNIEATRALAEISGLNVIASGGVHTLDDVLAARE
                     AKLAGVIIGRAIYDGSLDLRDALKEVSHAG"
     gene            complement(1479..2093)
                     /gene="hisH"
                     /locus_tag="VFQ23_15135"
     CDS             complement(1479..2093)
                     /gene="hisH"
                     /locus_tag="VFQ23_15135"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET9907983.1"
                     /translation="MSKIILIDAGTGNLRSVQKALESLGAVVERTEDPQKVVSGDRVV
                     LPGVGAFGDFMRGLRARGLEAALDDLVRRGTPLLGICVGMQALFEFGEEMGQHTGLGM
                     LTGNVKRFADSLAVKVPHTGWNQIEIREDVPLFRGIRSGDYVYFNHSFYCQPGNPQET
                     IATTSHGLSFACAVQRGNLFGVQFHPEKSQSVGLQLLKNFMEVT"
     gene            complement(2090..2683)
                     /gene="hisB"
                     /locus_tag="VFQ23_15140"
     CDS             complement(2090..2683)
                     /gene="hisB"
                     /locus_tag="VFQ23_15140"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET9907984.1"
                     /translation="MRIAELSRQTNETQINIHLNLDGTGKHEVTTGLGFLDHMLTHLA
                     VHGLFDLTVQAKGDLEVDAHHTVEDVALSLGQAFDKALGNRQGIRRMGDCFVPMDEAL
                     AHVVLDLSGRPYSVVQAEWHTPYIGPIPTTLFPHFFESFAVTARCNLHARVLYGRDDH
                     HQAEALFKAWARALDYATQLDSRRGDVVPSTKGTLTK"
     gene            complement(2876..>3468)
                     /locus_tag="VFQ23_15145"
     CDS             complement(2876..>3468)
                     /locus_tag="VFQ23_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9907985.1"
                     /translation="MPDAKLIDELLALPLLVVLDEAYIEFADSQLGARLSRISEVPSR
                     ENLVVLRTFSKWAGLAGLRIGYGAFPLWLMATLWKAKQPYNINVAASVAAQVSLTHAD
                     RLGTVVAKLQDERERLSSALQEIPYLQTFPTRANFILCRVLEKDASQLKLTLEREYGI
                     FVRYFNKPGLRDHIRISVGRPEDTDVLLNALKNISE"
BASE COUNT          839 a         1034 c          790 g          805 t
ORIGIN      
        1 acaggtagac cctgcctctg taattctgcc ttcaactccg gaattctcaa cttgccatcg
       61 tgaaacaacg atgcagccaa ggcagcatcc gctttgccat ctgtaagcac ctgtgcaaaa
      121 tgggatggcg accccgcacc accggacgca atcaccggaa ctgttactac ttctgaaatc
      181 gcacgggtta actccaggtt atatcccgcc aaggttccat cggtgtccat gctggtgagc
      241 aggatttcac ctgcccctaa ctcgacgccg cggatggccc attcaatggc gtccagacct
      301 gttggaattc gcccaccgtt gacaaatact tcccagcccg attccttttt gcgagcatca
      361 atcgccagca caatgcattg cgcgccgaac cgtgccgctc catcagacaa taattccggc
      421 cgttttaccg cagccgaatt gatgctcact ttgtctgcac ctgcgagcag gagattacgc
      481 atatcgtcca cttctctgat tcccccgccg accgtcagcg gcatgaacac tgaatctgcc
      541 acccgttcaa ccaactcaag tgtagctctg cggccttcgt gggttgcaga aatatcaagg
      601 aaaaccagct catccgcacc ctgagcatca tacaggcgcg cctgctctac cgggtcacca
      661 gcatcccgca aatttacaaa gttgactccc ttgaccacgc gtccatcttt gatatccaaa
      721 catggaatga ttctcttagc cagcatgaga aacctccttc agcgcgtccc gcaaatccaa
      781 agaaccatca taaattgcgc gcccgatgat cacacctgct aactttgcct cgcgtgcagc
      841 cagcacatca tcaagagtat gaacccctcc ggaagcgatc acattcaacc cactgatctc
      901 agccagggca cgcgtcgcct cgatatttaa tccactgccc aacccgtccc tgcgaatgtc
      961 tgtatagata acggtcacca gtccgagggt cctcatttgg atcgccaggt catttgctga
     1021 cgtgccacca tcctccttcc agccgcgcgt tcggacgatg ccatcgcggg cgtcaattcc
     1081 aacagcaatt cgttttgccc caaaacgcgc cagtgcctct tcaacaatag tggggtgttc
     1141 caccgcgacc gttccgagca cagcccgctc gacgcccagg ttcaacgttt gttcaatcga
     1201 aacaaccgaa cgtaatcctc cgccaaattg aacttgcgca ttgaattcct gagctgtcac
     1261 cagtatggat ttgagcgccg tttgattctc tgaatcgcgt tctccgaatg cactgtccag
     1321 gttcaccacg tgcagccatt gagcacccat gctgagccag cgccgcgcca tttcagaagg
     1381 atcatcactg taagacgtca tacgggcagg gtcgccttct tttaatcgca ccacttttcc
     1441 attgcgtaaa tcaattgcag ggaagatcgt aaatgatttc atgttacttc cataaaattt
     1501 ttcaacagtt gcaagccaac ggactggctt ttttccggat ggaattgaac accaaaaaga
     1561 tttccacgct gaaccgcaca ggcaaacgac aggccgtgac tagttgttgc gatcgtctcc
     1621 tgtggatttc ccggctgaca gtaaaacgag tgattgaaat agacataatc tccggaacgg
     1681 atcccgcgaa acaatggcac gtcctcccgg atttcaatct gattccagcc cgtgtgaggg
     1741 accttcacag cgagcgaatc tgcaaagcgc tttacatttc cagtaagcat gccgagtccg
     1801 gtgtgctgcc ccatctcttc accgaactca aacaatgcct gcatgcccac gcaaattccg
     1861 agtaatggag ttccgcgccg caccagatca tcaagcgccg cttccaaccc acgagcgcgc
     1921 aacccacgca tgaaatcccc aaacgcaccg acacccggca atacgactct gtctccactc
     1981 acaactttct gggggtcctc cgtgcgctcc actactgccc ccaggctctc caaagctttt
     2041 tgcacggaac gcagattgcc cgtgcccgcg tcaatcaaga tgatcttgct catttggtta
     2101 gtgtcccttt tgtggaagga accacatccc cgcggcgcga gtcgagctgg gttgcataat
     2161 ccagggcgcg tgcccaggct ttgaacaatg cctccgcctg atggtgatca tctcgtccgt
     2221 agagcacacg cgcgtgtaaa ttacagcgcg ccgtcacagc gaacgattca aaaaaatgtg
     2281 gaaacaacgt tgtcggaatc ggaccgatgt atggagtgtg ccattctgcc tgcacaacgg
     2341 agtatggacg cccggataga tcaaggacga catgcgccaa ggcttcatcc atcggaacga
     2401 aacagtcgcc catgcgccga attccctggc ggtttccaag tgccttatca aaggcctgtc
     2461 ccagcgagag cgcgacgtct tccacggtgt gatgagcatc tacttcaaga tcaccctttg
     2521 cctgaaccgt taggtcaaat aagccgtgga cagccagatg ggtcaacatg tggtccagaa
     2581 agccaagacc cgtagtcacc tcatgttttc cagtgccgtc caggttgaga tggatgttga
     2641 tctgcgtttc gttcgtttgg cgtgataatt ctgcaatgcg catgtttgct ccaatccccg
     2701 gtggtcgagt agtcccatcg cgctgagcgg agcgatagcg aagtcgaagc gtggttggga
     2761 tgtatcgaga ccaccaggtt tcaatatgta tattgctatt atgtaactga ctggtctcgt
     2821 gatgggccca cctgcactgc gtgcaggcgc aagtatcgcc ctactcgacc agcggttatt
     2881 ctgaaatatt cttcaatgca ttcaataaca catccgtatc ttccggcctt cccacactga
     2941 tgcggatatg atcccgcagg ccgggcttgt tgaagtaacg aacgaaaatc ccatattctc
     3001 gttcgagagt cagttttagc tgagatgcat ccttttcaag tacgcgacaa agaatgaagt
     3061 ttgcgcgcgt cgggaatgtt tgcaagtaag gaatttcctg taaggctgaa gatagccgct
     3121 ctcgttcatc ttgcaatttt gcaacaaccg tccctaatcg atctgcgtga gtcagtgaca
     3181 cttgagcagc aacactcgct gcaacattta tgttatacgg ctgcttcgct ttccataatg
     3241 ttgccatcag ccatagagga aacgcaccat atccaatccg tagaccagcc aggccagccc
     3301 atttactaaa cgtccgcaat accaccaggt tctcgcgcga tggcacttcc gaaatgcgac
     3361 tcaaccgggc gcccaattgg ctatccgcaa actcaatgta ggcttcatca aggaccacca
     3421 aaagtggaag cgccaacagt tcatcgatta attttgcatc tggcattg
//
LOCUS       DASTJP010000517        19345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1363156, whole genome shotgun sequence.
ACCESSION   DASTJP010000517 DASTJP010000000
VERSION     DASTJP010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363156"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFQ23_15150"
     CDS             <1..687
                     /locus_tag="VFQ23_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907986.1"
                     /translation="NIKPDVQERITFSVGNRYTILRDWLLAYREGMPLPLDHFFRKLF
                     GEVLSQAGFGYHSNFDAVRVAGSLVESVKNFRRAMEPSIVNKDRPDFDMGREYIAMLE
                     DGVIAASYLESWKSEDKDAVLVSPAHTFLMMNRPVTYQFWIDPGSNGWYQRLTQPLTH
                     PYVLSREWEPGRLWTDADEVEVNQQSMARLISGLLLRCRERVYLGISELGESGFEQRG
                     ELLRAFQKVL"
     gene            739..1890
                     /locus_tag="VFQ23_15155"
     CDS             739..1890
                     /locus_tag="VFQ23_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907987.1"
                     /translation="MKKISVLLILGIFIVSACTFNVEVLTPEAVIPTPPSPTETLVPL
                     PTPIATRTEPPFLSPTDAASLPQFSNTRFTLDVTTNLFQTVFPARIRRVYAVWDYQNM
                     RPGLIVRRDWYFNNVLWITREEPWDFSKYGENGTVQDISVFDLDVGLASGEYRLELYI
                     DMRSQPIGEGSWPLFTIAEADTESRVTSPDGRWVADVDNPRTLLVRDIGKSVRELYAG
                     NEITNVTWLPDSQHILFVDRDRSLQVKGLDVGILDDLWIVDVASGESHLLYKNTNQLG
                     KFSFSPDGRFAAGFDGSGFGDACLVDLRMIFFEFASDFRSVRTIKQDQFSGISAEPNI
                     VIYPVEEGTWQNETKYLVSLNGTCNFDQNLVGKYVFDLLELNAKKWGNN"
     gene            1901..2971
                     /locus_tag="VFQ23_15160"
     CDS             1901..2971
                     /locus_tag="VFQ23_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907988.1"
                     /translation="MIKPTSIALLFLCSALLFSCQSTDVGNSASANFTEGPVCEWPCT
                     EEDKLTAAAMFETPSALQTDIAPLTNLPALANCPMFPANNIWNARVDSLAVHAMSDAW
                     IDSIGRDEGFHMDFGSGEWDGGLIGIPYNVISGSTISKSTVDFYYPDESDGGPYPIPD
                     NPQIEYGSDHHILVVDTDTCILYEIYDASFDNSQWSGGSGAVWDLNSNALRPATWTSA
                     DAAGLPILPGLVRYDEILAGEIKHALRFTVENTAGYIWPARHFTSDPRDGIPPMGARF
                     RLKAGYDISGFPREMKVILQAMKTYGIILADNGSNWYVSGSPDERWDNGMLHLLDVLT
                     GGDFEAVDTSILMVDVDSGEAK"
     gene            complement(2988..4427)
                     /locus_tag="VFQ23_15165"
     CDS             complement(2988..4427)
                     /locus_tag="VFQ23_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9907989.1"
                     /translation="MTQKLNQSTVFLCLAVVTIGLAVFGYLGFYNRYWADDWCYNDDF
                     KHQGIVQSIGTYFDGENPHRGYPPNRYSFTIVSGLLYLPGIAGTQILATLTILLWVIA
                     LIWIGQNLNRFKSFTTTPVITLAATLLLFYTLNLSPHRFQILYWRSGVLPYSWTILAG
                     LYIVGVITSQLIKAQPSKSIIYMTGLISFFGGGFSEIGSAFLFSSITLLLLVAWFEKR
                     RSKEWALKTFSTIRTAWVFLLLGLIALAVSPSNARYVDLNRNPNDPFVVPFLSLRYAF
                     DFILYSLLGLPLPHAILFAAFVAIGILSGNSNNSRLEIKRSAWFILLTIFVTYLLVTA
                     IEAPTTYLYSAPPDPRGQSLARFVVLTGMSIAAWMIGASMSPKVNGIFTVLPAIILLA
                     VSAYTLRATLNVYKELPGFVERADLWDQRDAQIQDAIGQGITQIEIIPIDTKDINTRD
                     IIRSEAFGKWVTDACGVQYYDAEAMRVAP"
     gene            complement(4424..5239)
                     /locus_tag="VFQ23_15170"
     CDS             complement(4424..5239)
                     /locus_tag="VFQ23_15170"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HET9907990.1"
                     /translation="MQLLYGFLIAALVSILAFRSHSLDRSGALSAAVMGTIVFGLGGL
                     QWAILLLAFFITSSAFSRAFKNRKQGLSEKFSKGNQRDAGQVFGNGGIATVFVLIHAL
                     YPESPVGWVGFAASLAAVNADTWATELGVLNPTRPRVITDLRKHVEKGTSGGISLLGT
                     LASLLGAALIGILAALFNPAEASISLDWSLALIITFAGLAASLFDSFLGATVQAMYYC
                     PTDQKETEKHPLHTCGTRTIHRRGWAWLNNDLVNFACAVFGSVTALILTSTLQ"
     gene            complement(5298..6215)
                     /locus_tag="VFQ23_15175"
     CDS             complement(5298..6215)
                     /locus_tag="VFQ23_15175"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9907991.1"
                     /translation="MNNTRVGHFPPAKRLGLIVHGIIVLLLAAISGWGFFNLTLAPVG
                     PTFVSYLLTALLAFAPIPFFGYRGYALLRADYYIDRDSLAILWGLRVEDIPLTDIEWV
                     RPATDLSHPLVLPRIRLPGAVIGTRRHVDLGPVEFIASDSNKLILIATSKRVFAISPN
                     DMAALVRTFARATELGSLTPAEAKSVYPSFVITQAWDSLIARFLWVSGLILNLGLFVW
                     VGLLIPSLPEIPIGFNSAGIPGEPTSSVRLILLPFVSASMFTIGVFGGLYFYRWEMQR
                     PLAFILWVSSTLCTVLFLLAVLFLVTASS"
     gene            complement(6254..7453)
                     /locus_tag="VFQ23_15180"
     CDS             complement(6254..7453)
                     /locus_tag="VFQ23_15180"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9907992.1"
                     /translation="MKYRDLDIQTQREAPNNARTEGFSFLVRAGYLTRDNTPTKLGEY
                     AINHLRKLSETPSFLSRLSLPVISTDREIFLPIASGNVEVAHCESCKYTSRIELAKFS
                     KSIGVEQVPTLPLEKVFTPNCNTIEALANFLDIPKEKTAKALMYTRLSDSKFVFVVVR
                     GDMQLSEAKLRQQVGDIRPATLEEIEKSGAAAGYASPIGLKDAVIVVDDLVPLSTNLV
                     AGANETGYHFKNTNSGRDYSAEIVADVTQAKAGDKCLTCQNPLSVGSTLTLAANEKYN
                     LEFVLLALAETYHDAKGLTFSHPAAPFDVYLMHIPGKELETGAAATEIYGTLQSAGIS
                     VLFDDRDERAGVKFNDADLIGCPLRVTVGEKGLKDRMVELKPRHIKENQLISIDKVTE
                     EIKSFLK"
     gene            complement(7502..8023)
                     /locus_tag="VFQ23_15185"
     CDS             complement(7502..8023)
                     /locus_tag="VFQ23_15185"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9907993.1"
                     /translation="MFKRNTTPTETQQPIQAVERITSVLGSGVIWHGSINGSGGVRIE
                     GAFEGEIALRGMLVVGETGRVTCQNVRANTVIVAGAVRGNITTQKLEIRGSGRVWGDV
                     VTTAFVTEEGAFLRGQIRMEETVELDLEPAPETTPSEAAQAESIASTPLVIPEQMSGG
                     TTRRTVRKKKEEE"
     gene            complement(8128..8928)
                     /locus_tag="VFQ23_15190"
     CDS             complement(8128..8928)
                     /locus_tag="VFQ23_15190"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HET9907994.1"
                     /translation="MGLLEGKNALIFGLANDKSIAWGITQAFHREGATLGISYAGEML
                     ERRVKPLAEKVGCQWVEECDVTKDDEIAATAEKAASHFGKIDVLVHSIAFAGRDELSK
                     PYYQTSRDGFRNTFDISVYSLVALTNAFLPFMNPGGSIMCLTYYGSVKVAPNYNVMGV
                     AKAALESSTRYLAFDLGPKKIRVNAISAGPIRTLSAAGVGGFRDMYKHFADAAPLREN
                     VTIEDVGNAAVFLASNLSAKITGEVLYVDSGFNTVGVQIAAKEESKEP"
     gene            complement(8975..9559)
                     /locus_tag="VFQ23_15195"
     CDS             complement(8975..9559)
                     /locus_tag="VFQ23_15195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET9907995.1"
                     /translation="MTRILPRSFYNRPTLTVARELIGARLVRILDGVKLVGLITETEA
                     YISQKDLACHAKAGLTQRTAVMFGEPGHAYVYFTYGNHWMLNAVTEREGFPAAVLIRA
                     IQPMEGTEIMLERRSGRDTYGPGKLTQALGITKSENGVDLTESTHGLWIEEGVHVPNS
                     LVTKGPRVGLNNTPEPWLSKPWRFLIKGWKINSL"
     gene            complement(9572..10495)
                     /locus_tag="VFQ23_15200"
     CDS             complement(9572..10495)
                     /locus_tag="VFQ23_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9907996.1"
                     /translation="MNFLITGAAGFLGSSLANQLSREGHQVRGIDDLSTGTPQALAPD
                     VHFTRGDVSDRPKLWTLLQEVDVVYHLAARVSVPESVLYPRDYNNVNVGGTVALMEAM
                     RDVGVRRVVLTSSGAVYGDLAEQPLRESVTPNPRSPYAVSKLAAEYYVRTIGGLWGIE
                     TVSLRIFNTYGPGQHMPPSHPPVVPHYLKQAQRGGTLVAHGDGSQTRDYVYVDDVVSA
                     LIAAATAPDVNGLVINVGSGTETSISDLIKHVLAATNSNPEVLYNATTPGGVSRMRAD
                     LSLAHEKLSYRPSIKLDQGLRLTLKRDQRFK"
     gene            10746..10958
                     /locus_tag="VFQ23_15205"
     CDS             10746..10958
                     /locus_tag="VFQ23_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET9907997.1"
                     /translation="MSERITGTVKWFNGDKGFGFIEREGGKDVFVHFSAIQGDGFRNL
                     TEGQKVEFNVEQGPKGPQASNVTMSN"
     gene            complement(11015..11758)
                     /locus_tag="VFQ23_15210"
     CDS             complement(11015..11758)
                     /locus_tag="VFQ23_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9907998.1"
                     /translation="MRIAVISDIHGNNFALEIVLADIKEKSVDQIVCLGDAIQGGSQP
                     AEVVQNLRALNCPVVMGNADAWLLSGEETGDEGIPPERLKKMGEIRLWSLSKLSEDDR
                     TFISGFQATVTINSDQGPDLLCFHGSPTSFDDIILPHAPKEVFDKYLGAYSKHILTGG
                     HTHAQQVRRNGDQFFFNPGSVGFAYSHYQADGQFKADASAEYAILTFENGKTSLEFRR
                     LPFDAGELFRIFRESGRPFAEEAIAQYQS"
     gene            11902..12822
                     /locus_tag="VFQ23_15215"
     CDS             11902..12822
                     /locus_tag="VFQ23_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9907999.1"
                     /translation="MRKWSIFILLWGFVLSPSTAGAQGGTQLQAVNIELWSEYDQPSM
                     LVIEEFVVTEDTTLPAEVTLRFHNDAHLIAVAFTSEGQLLNAEFDGPRDHGDWQVLDV
                     NVRTRDIYRIEYYQPLIQEGNKRKFSFEWLGDYSVKQFNLSAFIPSDSANIVTAPVLS
                     SQSSLPKGLYLTNEEGFGKIRMGESYEFEIEYTRDSTMLTNATQGVDVQASDPVGPNT
                     PGRTSIDKLPWIIGGFGLALIAVILFLFSYWRSMQSEIHAAATLARTKLGRRRGKKGE
                     NVQIYCHECGTRAQTGDRFCRVCGNKLRVE"
     gene            complement(12838..13350)
                     /locus_tag="VFQ23_15220"
     CDS             complement(12838..13350)
                     /locus_tag="VFQ23_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908000.1"
                     /translation="MDHATWVNSLLDSVVEIGEEGKAAVDFIRTRRTQIGIKKARPNV
                     GAFWTVFGNIRLNSHYYTYETPLDDLRIKTLIIHEARHLQQGLLTALSVYGELDAWQL
                     EFRVYHRIKGKYPHSAIEELIKLPLEYDRAVLKKAVQLMQAYAGKGYRIDLLPLYPLG
                     REIKYWIARK"
     gene            13572..14342
                     /locus_tag="VFQ23_15225"
     CDS             13572..14342
                     /locus_tag="VFQ23_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908001.1"
                     /translation="MLKTKFLSSILLAITILFAQTGIAAAAPALQDTTPITGTIQTMS
                     VETDASGLTTVLVTLLDDQGATQTVRLSVETAVELGLVALDPVTNEPVVDETQIGLTV
                     EVDPTSVIPDEPVEETAHPIAILLATFFEEDVSVVNDYHEDGFGFGVIAQALWMSQNL
                     NEDASLAGLILEAKESGDYSAFTLPDGSTPTNWGQFKKAVLEKKNNLGVVVSGQAETD
                     QDSTLTTTAEDEHGNGKDKDKGKNKNKNNNNRNNGNRP"
     gene            14444..15601
                     /locus_tag="VFQ23_15230"
     CDS             14444..15601
                     /locus_tag="VFQ23_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET9908002.1"
                     /translation="MREVAILGIGQTKIDEHWDLSLREIGGNAAFAAMQDAGMDKVDA
                     LFVGNMLSPMIDGQNQLGAYFSDWIGLWKQEAVKVEAACASGAAALRAGLMAVSAGDI
                     ESALIVGVEKMTDKAGHDITAALATAADADYELEQGISFVGINALIMRRYMHEFGWAH
                     ADFAPFSINAHANAIHNPFARLHQKITVDQFEKSSMVATPINLMDASPIGDGAAALII
                     VPAEKVASLAGKPRVTVAGSASATDSIAVHSRKDPLFLSAAYQSAKRAYEMASVELDE
                     IDVFELHDAFSIMSALSLEACGFAERGQGVRLGLDNEISPNGRVPVCTRGGLKARGHP
                     VGATGIYQIVEVVQQLRGECGATQVDGARVGMAQNIGGSGATIITHILKAN"
     gene            complement(15609..15941)
                     /locus_tag="VFQ23_15235"
     CDS             complement(15609..15941)
                     /locus_tag="VFQ23_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908003.1"
                     /translation="METKLPSRKPILIWLIVSQLLALASLVVWLLMAGLSVMAFDSGV
                     TQEAWNFVIAVWLYPIWPILFAIAAWIAYWRKRDRLAGILTTFTFLPILVLLLLIIGA
                     NSLYFLNT"
     gene            16170..16562
                     /locus_tag="VFQ23_15240"
     CDS             16170..16562
                     /locus_tag="VFQ23_15240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9908004.1"
                     /translation="MPNSNKRKVGRRDFTYYMQVTDDISKQLIGYLSDISTGGFKLDS
                     QKEISPGQDFRLQIELTPDIAEKNSMVFIARSKWCHRDHVDPNTFNVGFEIINISPSD
                     MGIFQRMFDKYGSQTSTRAKGADDFMWR"
     gene            16666..17340
                     /locus_tag="VFQ23_15245"
     CDS             16666..17340
                     /locus_tag="VFQ23_15245"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9908005.1"
                     /translation="MKFQTLRSIIRFFMNLITDIEVHGIEKLPAGNVIAAANHLGRLD
                     TAALLCIIDREDIIMPVAEKYRHHLIYGAMGRAVDAIWLNRFEADYAALRQILERMKK
                     GGMLVIAPEGTRSKTEALQEAKLGVAFLASKSGYPVLPVAVTGTEDRGILENLKRFRR
                     SKIVVKAGELFYIQVPPGKGREEAMRQATDEIMCQIGVLLPEKYRGVYANHPRLKELE
                     RDHLPV"
     gene            17345..18007
                     /locus_tag="VFQ23_15250"
     CDS             17345..18007
                     /locus_tag="VFQ23_15250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9908006.1"
                     /translation="MIRVLRFLIRILLKLIARFEIRGKENVPLTGGMILASNHMGILD
                     ILMVYFGIDRTDLFIPVAEKFEKIGWIRWISKYLNFLFVDRFNPDLKAIRKMIALMEE
                     GKCLVIAPEGTRSLTGSLNEGKPGVAYLAARSGFPVIPIAITGTPDKVVIENVKKFRK
                     SHITLTGGKPFVIPPLPNKDRDVALQKYTDEIMCQIAALLPEQYRGVYADHPRLKELL
                     SE"
     gene            complement(18027..18404)
                     /locus_tag="VFQ23_15255"
     CDS             complement(18027..18404)
                     /locus_tag="VFQ23_15255"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET9908007.1"
                     /translation="MTTKKPVIHCDALVVHCMDYRLQKFLQPWITVRFGYDNFDIISL
                     AGSVHDYEMVLKYVQLAVRIHSIEVVCLINHEDCRAYGREGTYKRHRHDLLDTGAKIR
                     ALFPQLNVETFYLHLDGNFEQIE"
     gene            complement(18407..>19345)
                     /locus_tag="VFQ23_15260"
     CDS             complement(18407..>19345)
                     /locus_tag="VFQ23_15260"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9908008.1"
                     /translation="DDGSIFKALRIARENGMLMMAHCENGDVIETLVAEAIAAGHTSP
                     EYHTLTRPAWGAIEATLRMAAMAEQADSPVYIVHMNAGGEVDMLQYARERGVKVMGET
                     CPQYLFFTIEDLRRADGAKWICSPPMRTRQDNTRLWEGLTDGVMQTIGTDHCPFFFDG
                     SSPIVYEGKEIAIPGKELGKNDFTKIPNGLPGVQDRMPILWTYGVRAGKITPNQFVAY
                     MSTNPAKIFGLYPRKGALMEGSDADIVIWDPEKTVKYGVAHSHHRTDYNLYEGWELTG
                     YPEKVFLRGKRIVDGEQWLGKAGGGQFLKRGHGEVL"
BASE COUNT         5000 a         4627 c         4861 g         4857 t
ORIGIN      
        1 aatatcaagc ctgatgtgca ggaacgcatc acgtttagtg ttggcaaccg atatacgata
       61 ttgcgagact ggcttcttgc ttaccgggaa ggcatgccat tgcctctcga tcatttcttc
      121 cgaaaattat ttggagaggt gttgtcacag gcaggctttg gatatcacag caatttcgat
      181 gctgtgcgcg tggcggggag cttggtcgag tctgtaaaga attttcgcag agccatggaa
      241 ccctcgatcg tgaataagga tcgccctgat tttgacatgg gcagggaata tatcgccatg
      301 ctggaggacg gcgtgatcgc cgcatcgtac ttggaatcct ggaagagcga ggacaaagat
      361 gcggtgctcg tctcaccggc ccatacgttc ctaatgatga atcggccagt gacatatcaa
      421 ttctggatcg atccgggctc taacggatgg taccagcgcc tcacacaacc gctcactcac
      481 ccgtacgtct taagtcgcga gtgggaacca gggaggttgt ggaccgatgc ggatgaggtg
      541 gaagtgaatc aacaatcgat ggctcgcctg atatcgggcc ttctgttgag gtgccgcgag
      601 cgggtctatt tgggaatttc agagttaggg gaaagcggct ttgagcagcg aggagaactg
      661 ctgcgggcct tccaaaaggt actatgagtt gggaacaggg gcgcattgac cgacgtttat
      721 aggcaatacg gagtcaggat gaaaaaaata tctgtcttgc ttattctcgg tattttcatt
      781 gtgtcggcct gtactttcaa tgtggaggtc ttgacacccg aagcggtgat tccaacaccg
      841 ccgtcaccca cagaaacgct ggtcccttta cccaccccaa tcgcaacgag aaccgaaccg
      901 ccatttttat cgccgactga tgctgcatcg cttcctcaat tttcaaatac gcgcttcacg
      961 ctggatgtaa ctaccaacct ttttcaaact gttttccccg cccgaatacg ccgggtgtat
     1021 gccgtctggg attatcaaaa catgcgcccg ggattgatag tgcggcggga ttggtatttc
     1081 aacaatgtgt tgtggatcac acgtgaggaa ccctgggatt tttccaaata tggggaaaat
     1141 ggaaccgtgc aagatatttc agtttttgac ctggatgtag gtcttgcatc tggagagtac
     1201 cgtctcgagt tatacattga tatgcggtcg cagccgattg gagaaggcag ctggccgctg
     1261 ttcacaattg ctgaggctga cacagaatcc agggtcacat ctccggatgg acgatgggtc
     1321 gcggatgtgg acaaccccag aaccctgctg gtcagggata tcggtaagag tgtccgcgag
     1381 ttatatgccg gaaatgaaat tacaaacgtg acctggctgc cagacagcca acatatactt
     1441 ttcgtagacc gggatcgttc actacaagtt aaaggactcg atgtaggaat tttggatgat
     1501 ctttggatcg ttgatgttgc cagtggcgag tcccacttgt tatacaaaaa tacaaaccaa
     1561 ctggggaaat ttagcttttc gcctgacggt cgttttgcgg ctggtttcga tgggtcaggt
     1621 tttggggatg cctgccttgt tgatttgcgt atgatttttt ttgaattcgc cagcgatttt
     1681 cgaagtgtac gaacgatcaa gcaggatcag ttttccggga tttcagctga acccaatatc
     1741 gttatttatc ccgttgaaga aggtacgtgg caaaacgaaa caaaatatct tgtttcatta
     1801 aatggtacat gtaattttga tcagaacctg gttggtaaat atgttttcga cctattagag
     1861 ttgaatgcaa aaaagtgggg caataattaa ggatgtcagc ttgattaagc ccacatccat
     1921 cgcgttgtta ttcttgtgct cagcacttct tttttcctgt caatcgaccg atgttggtaa
     1981 ctcggcatca gcgaatttca cagagggacc ggtttgtgag tggccgtgca ccgaggaaga
     2041 caagctgact gcagccgcga tgttcgagac gccttctgct ttgcaaaccg acatcgcgcc
     2101 gctaaccaat ttaccagccc ttgcgaactg cccaatgttc cctgcgaata acatatggaa
     2161 cgcgcgcgtc gactcacttg cagtccatgc tatgtcagac gcgtggatcg acagcattgg
     2221 gcgcgacgaa ggctttcata tggattttgg atcaggcgag tgggatggcg gcctcatagg
     2281 gataccatac aatgtgattt caggttcaac gataagcaaa tcgacagttg atttttatta
     2341 ccctgatgaa tcagatgggg gaccttatcc gattcccgat aatccacaaa ttgaatatgg
     2401 ttctgaccat catattctcg ttgtggatac agacacttgc atattgtatg aaatttacga
     2461 cgcgagtttt gataatagtc aatggagcgg cggctcgggg gcggtttggg atttgaactc
     2521 caacgcctta cgccctgcca cctggacctc ggccgatgca gcaggtttgc cgattctgcc
     2581 gggtctggtt cgatacgatg aaatcctggc tggcgagatc aaacacgctc tgcgttttac
     2641 cgtcgaaaac acagcagggt atatctggcc cgcgcgacat ttcacatccg atccacgaga
     2701 cggcatccca ccgatgggcg cgcgcttccg tttgaaggct ggttatgata tttcagggtt
     2761 ccctcgtgag atgaaagtga tcttacaggc aatgaaaaca tacggaataa tattggctga
     2821 caacggctcg aactggtatg tgagcggttc accggatgaa cgctgggata acggcatgct
     2881 gcacctattg gatgtactga caggcgggga tttcgaagcg gttgacacct caatcttgat
     2941 ggtagatgtg gattcgggcg aagcaaagta actgatttac ctggcttcta cggtgcgacc
     3001 ctcattgcct cagcatcata atactgaact ccacaggcat cggtaaccca ttttccgaag
     3061 gcttccgaac ggataatatc tcgtgtgttg atgtcttttg tgtcaattgg gataatttcg
     3121 atttgtgtga ttccctgtcc gatggcatct tggatctgcg catctctttg atcccaaagg
     3181 tcggcgcgct cgacaaaacc gggtagttcc ttgtaaacat tcagggtggc tcgcagcgtg
     3241 taagcgctta cggccagtaa gatgatggca ggcaaaactg tgaagatgcc attcactttt
     3301 ggcgacatac ttgcgccgat catccacgcc gcgattgaca taccagtcaa caccacaaag
     3361 cgagccagag actggccgcg cgggtctgga ggcgcgctat agagataagt agtgggagct
     3421 tcaatggcag ttacaagcag ataagtaacg aaaattgtca gcaggataaa ccatgctgac
     3481 cttttgattt ccagcctact attatttgaa ttaccagaca gaatgccgat cgcaacaaaa
     3541 gctgcgaaaa ggattgcatg tgggagtggt aaaccgagaa gagaatacaa aatgaaatcg
     3601 aaggcatacc gcagagaaag gaaaggaact acgaaaggat cgttcggatt ccgattcaga
     3661 tcaacatagc gagcattgga tggtgaaaca gcgagggcaa ttaatcccaa taagagaaaa
     3721 acccatgcgg tacggatggt tgagaatgtc tttagcgccc actcttttga ccttcgtttt
     3781 tcaaaccatg ctacaagcaa caaaagagtg atgctgctaa acaggaatgc cgatccaatt
     3841 tctgaaaagc ccccaccgaa gaaggaaatc aaacctgtca tataaatgat gctttttgaa
     3901 ggctgtgctt ttatcaattg actggtgatc actccaacga tatataaacc tgcaaggatt
     3961 gtccagctgt agggtaaaac tccagaacgc caatacagga tctggaatcg gtgcggagac
     4021 agattcaacg tgtaaaaaag caatagggtg gcagcaagtg tgatgaccgg agtagtcgta
     4081 aacgatttga agcgattcag gttttgcccg atccagataa gtgcgatgac ccataaaaga
     4141 attgtcaaag tcgccaggat ttgagtccca gctattccag gcagatataa caacccagat
     4201 acgatggtaa aagaataacg gttgggtggg tatcctcgat gtggattctc cccgtcgaaa
     4261 taagttccga tcgattgaac aataccctgg tgcttgaagt catcgttgta gcaccagtcg
     4321 tcagcccaat agcggttgta aaacccgagg tatccaaaaa ctgccagtcc gatggtaacg
     4381 actgccaaac agagaaaaac cgtggactga ttaagttttt gggtcattga agcgttgaag
     4441 taaggatcag tgcagttact gaaccaaaca cagcacaagc gaaattgacc aggtcattat
     4501 ttagccatgc ccagccgcgc ctgtgaattg tcctggtgcc gcacgtgtgg agtggatgct
     4561 tttcagtctc tttttgatca gtggggcagt aatacattgc ctgaactgta gcgccgagaa
     4621 atgagtcgaa cagtgaggct gcgagtccgg cgaaggtgat tatcaatgcc agagaccaat
     4681 caaggctgat cgacgcttcg gcaggattga acaacgccgc caggattccg ataagagccg
     4741 cgcccaaaag cgaggccagt gttccaagca gcgagatccc gccagaggtc cctttctcga
     4801 catgctttct taaatctgtg atgacgcgcg gacgagtcgg attcaagact cccaactccg
     4861 ttgcccaggt gtccgcattg actgctgcca atgacgcggc gaagccgacc cagcccacag
     4921 gtgactcggg gtaaagagca tggatcaata caaagaccgt tgcgatgcca ccatttccga
     4981 agacctgccc ggcgtcacgc tgatttccct ttgaaaattt ttcgctgagt ccctgcttgc
     5041 gattcttaaa cgcgcggcta aatgcagagg atgtgatgaa aaaagccagt aacagaattg
     5101 cccattgcaa tccgccaagc ccaaagacga ttgtccccat caccgctgcg gataaggcgc
     5161 cgcttcgatc gagactgtga gaacgaaacg ccagaattga aacaagggct gcgatgagaa
     5221 aaccgtaaag aagttgcata atcttcaatc gagagtcagg gaaattttaa tgtcgctcaa
     5281 cgttgctcga tccttcgtta gcttgaggcg gtcaccagga acaataccgc cagcaaaaac
     5341 aacactgtac agagtgtgct ggatacccaa agaatgaacg caagtggacg ctgcatttcc
     5401 caacgataga aatataagcc gccaaagacg ccgatagtaa acatcgatgc actgacaaaa
     5461 ggaagcagaa ttaaacgaac agaggaggtg ggttcaccag gtattcctgc tgaattaaat
     5521 ccgattggaa tttctggcaa agatggaatc aagagtccta cccaaacgaa gagaccaagg
     5581 ttgaggataa gtccgctcac ccatagaaat cgtgctatca gactgtccca ggcctgcgtg
     5641 ataacgaaag aaggatacac cgacttggct tcagccggtg tgagactgcc aagttctgtg
     5701 gcgcgggcga acgtgcgaac cagggctgcc atgtcatttg gtgagatggc aaaaacacgt
     5761 ttggatgtag caatcaggat cagtttattc gagtcggatg cgataaattc aaccggaccc
     5821 aggtctacat gtcgacgtgt tccgatcacc gcgcccggta agcgaatccg gggcaaaacg
     5881 agtggatgtg aaagatccgt agctgggcgg acccattcaa tatcagtcag tgggatatcc
     5941 tcgacgcgca gcccccataa gatcgcaagg ctgtctcgat caatataata atctgcgcgg
     6001 agaagggcat agccgcggta tccgaagaat gggatagggg caaaagcgag caacgcggtc
     6061 aacaaatagg agacgaacgt tggacctaca ggggcgaggg tcagattaaa aaatccccag
     6121 cccgaaatag cagcaagtag taaaacgatt atcccgtgta cgatcaagcc caatcttttc
     6181 gcaggcggga aatgtccgac tctggtattg ttcataaggg tcttgaagtg gcgaccagcg
     6241 cgctgccact acattatttc aaaaaggatt tgatttcttc agtcacttta tcaatcgaaa
     6301 tcagttgatt ctctttgatg tgacgcggtt tcaattctac cattctatct tttagaccct
     6361 tttctcccac tgtaaccctg agcgggcagc cgatcaaatc tgcatcattg aacttgaccc
     6421 ctgctcgttc gtcgcgatca tcaaaaagta cagaaattcc tgcgctttgc aaagtgccat
     6481 aaatctctgt tgcggctgcg cccgtttcca gttccttgcc agggatgtgc atgagataca
     6541 cgtcaaacgg cgcagcggga tgggagaagg taagaccttt tgcatcatga taggtttccg
     6601 ccagagcgag aagaacaaac tctaagttat atttctcgtt ggcagcaagt gtgagcgtag
     6661 aacccactga aagtggattt tggcaagtca aacacttatc tcctgccttc gcctgagtca
     6721 catctgctac gatttcagcc gagtagtcac gtccagaatt ggtgttcttg aagtgatacc
     6781 ctgtctcatt ggcacctgct accagattcg ttgaaagcgg gactagatca tcgacaacga
     6841 tcactgcatc ttttagtccg attggggagg cataccctgc tgcagcacct gacttttcga
     6901 tctcttcaag agtcgcaggt cggatatctc caacctgttg ccttagcttc gcttcgctga
     6961 gttgcatgtc gccgcgtacg acgacaaaaa caaatttact gtcagagaga cgcgtataca
     7021 tcaaagcctt agccgtcttc tctttcggaa tgtcaagaaa gttggcgagt gcttcaatcg
     7081 tgttgcaatt tggcgtgaag actttttcca acggtagtgt tggaacttgc tccaccccta
     7141 tggattttga aaactttgcg agctctatcc tactcgtgta tttgcaggat tcacaatgcg
     7201 cgacctcaac attcccagat gcgatgggta ggaagatttc acggtcagtg ctgattactg
     7261 gcaacgaaag acgagagaga aaggatgggg tttcagacaa cttgcgtaaa tgattgatcg
     7321 catactcccc taattttgtg ggagtgttat cgcgtgtcaa gtagcctgcg cggacgagga
     7381 aggaaaagcc ttctgttcgg gcattgttcg gggcttcgcg ttgggtttgg atatcaaggt
     7441 cgcgatattt cataaaataa ttagtagggg cgaccagctg gtcgccccta cgatgggata
     7501 attattcttc ttccttcttc tttcggaccg ttcttcgagt ggttccccct gacatctgct
     7561 caggaatcac gaggggtgtg gatgctatcg attctgcttg agccgcctct gaaggtgtag
     7621 tctcgggcgc aggctcgaga tccagttcga ccgtttcttc catccgaatt tgacctctca
     7681 gaaatgcgcc ttcctccgtc acaaaagcag ttgtgaccac atctccccac acgcgaccag
     7741 agccgcggat ctccaatttt tgagttgtga tattgccgcg taccgcgcct gcaacaatca
     7801 ccgtgttagc gcgcacattc tgacaggtca cacggcccgt ttcaccgacc accagcatgc
     7861 cgcgcagggc aatctcgccc tcgaacgcgc cctcaattcg tacaccgcca gacccattga
     7921 tggaaccgtg ccagatcact ccagagccta gcactgatgt tattcgttcg acagcttgta
     7981 tcggttgttg tgtttcagtg ggagtagtat tgcgtttgaa cataatagta taaccacgaa
     8041 ggaattagga tcaccaatga acaaaattgg gcgcgaaacc ttcgcgaccc aattttatca
     8101 tcttagcgtt ctttgcgtcc tggcggttta aggctcttta ctttcctctt ttgcagcaat
     8161 ttgcacgccg actgtattga agccggaatc tacatagagc acttcaccgg taattttagc
     8221 agatagattt gatgcaagaa acacggctgc attgcctacg tcctcgattg tcacattttc
     8281 acgtagggga gcggcatctg caaagtgctt atacatatcg cggaacccgc caacacccgc
     8341 agcggagagt gtgcgaatgg ggcctgcaga aatggcattg acacggattt ttttgggacc
     8401 caggtcaaaa gcaagataac gtgtggagga ctcaagcgca gctttggcga ctcccatcac
     8461 gttatagttt ggagcgacct tcactgaacc gtaataggtc agacacataa tcgaaccgcc
     8521 ggggttcatg aacggcaaaa acgcgtttgt gagcgccaca agcgagtata cgctgatatc
     8581 aaaagtgttc cgaaatccat ctcggctggt ttgatagtat ggcttgctta attcatctct
     8641 gccggcgaat gcaatcgagt gtaccaacac atcgattttc ccaaagtgac tggcagcttt
     8701 ctcagcagtg gcagcaattt catcatcctt ggttacatcg cactcttcca cccactggca
     8761 acccactttc tctgcaagcg gctttactct tcgttccaac atttcaccgg catagctgat
     8821 accaagggtc gcaccctcac gatgaaacgc ctgtgtgatg ccccaggcga tggacttgtc
     8881 atttgccaga ccaaatatca acgcattctt gccttcaagt aaacccattg ttcctccatt
     8941 aatttttcct gtagtatacg cccgctgccg tgcactacaa agaattgatc ttccaacctt
     9001 taattaagaa cctccatggc tttgagagcc agggctccgg cgtattattt aaacccacac
     9061 gcggaccttt cgtcacgagc gaattcggga cgtgtacgcc ctcttcgatc cataaaccgt
     9121 gagttgattc cgttaaatca actccattct cacttttagt gattccgagc gcctgcgtca
     9181 acttgccagg tccgtaggtg tcgcgtccgc ttcgccgctc aagcataatt tctgtccctt
     9241 ccattggctg tatcgcacgg atcaacacgg cagccggaaa tccttcgcgt tcagtgacgg
     9301 cgttcaacat ccaatgattg ccataggtga aatagacata ggcatggccc ggctcgccaa
     9361 acataaccgc tgtacgctgc gtgagtccgg ctttcgcatg gcaagccaga tccttctggc
     9421 tgatgtaggc ttcagtttct gtaatgagac caacgagctt cacgccatcc aaaatccgaa
     9481 ctaatcgtgc accaatcagc tcgcgggcta ccgtcagtgt aggacggtta tagaaggaac
     9541 gaggaaggat tctagtcaca gatttggaaa gttatttaaa tctttgatca cgtttcagag
     9601 tcaatctcaa accctgatcg agtttgatgg atgggcgata gctaagcttc tcgtgtgcca
     9661 ggcttaggtc tgcccgcatg cgtgaaacgc cgccgggggt cgtggcattg tacaacactt
     9721 caggattact attggtagcc gcaagaacgt gtttgatcaa gtcgctaatg cttgtttcgg
     9781 ttccagaacc gacattgatc actagcccat taacatccgg tgcggtcgcg gctgcgatga
     9841 gcgcgctgac cacatcatcc acataaacgt agtcgcgggt ctggcttcca tcgccgtggg
     9901 caacaagagt tcctccacgc tgtgcctgct ttagataatg tggcacaaca ggtgggtgag
     9961 aaggcggcat atgttgaccc ggtccatagg tgttgaagat ccgcaagctg actgtttcga
    10021 ttccccataa cccaccgatc gtgcgaacgt aatattctgc cgccagtttc gaaactgcat
    10081 acggcgaacg cggatttggc gtcaccgatt cgcgaagcgg ctgttcggcg agatcaccat
    10141 agaccgcgcc cgaggaagtc agcacaacgc gtcgcacgcc tacatcgcgc atcgcttcca
    10201 tcagagcaac ggtcccgccg acgttgacgt tgttgtagtc acgtggatac agaacagact
    10261 caggaacgga gacgcgtgcc gcaaggtggt agaccacatc cacttcctgt aacagagtcc
    10321 acaacttggg tcggtcgctt acatccccgc gtgtaaaatg gacatcaggt gcgagcgcct
    10381 gcggagtacc cgtagagaga tcatcgatgc cgcgtacttg atgcccttca cgcgacaatt
    10441 gattggcaag cgaagagccg aggaatcccg cggctcctgt aatgaggaag ttcataagtg
    10501 gtggaaatta tagcaccagg agtatttctc tcttggtaga aggtcaatct aaaaattgat
    10561 cacgaaatta gattgactca tcgctactag tatgttattc ttgttcttgt aggataacgg
    10621 taagttacgt tggtttgaag aacagccaca gctcacggtt tgaatccgtg ggctgttttg
    10681 ttttgacctc cgatggaaca aacaggatgc ctaaaatata taaattgcca aaggaggcaa
    10741 atacaatgtc tgaaagaata actggtacag tcaagtggtt caatggtgac aagggcttcg
    10801 gcttcatcga gcgcgaaggt ggaaaggacg tattcgtcca tttctccgcc attcagggcg
    10861 acggtttccg taacctgacg gaaggtcaga aagtcgagtt caatgtcgaa caaggaccca
    10921 agggaccgca ggccagcaac gtcacaatga gcaactagtt gacaaaagaa gagcctcgcc
    10981 aaacggcgag gctcttttga ttcatccatt caaattagct ttgatattgg gcgatcgctt
    11041 cctccgcgaa cgggcgcccg ctctcgcgaa agatgcgaaa taattcgcca gcatcaaatg
    11101 gcagccggcg gaactcaaga ctcgtcttgc cgttctcaaa ggtcagtatt gcatattctg
    11161 ccgacgcatc cgccttgaat tgaccatccg cctggtagtg gctgtaggca aagcctacgc
    11221 tcccggggtt gaagaagaac tgatccccat tccgacgcac ctgttgagca tgcgtatgac
    11281 cgcccgtcag gatatgtttg gagtaggcgc caagatattt gtcgaacacc tctttggggg
    11341 catgaggcag gataatatcg tcaaaggaag tgggggagcc atgaaagcat aacaggtccg
    11401 gcccctgatc agaatttatc gtgaccgtcg cttgaaagcc cgaaataaaa gtgcggtcat
    11461 cctcactcag tttggacagc gaccacaggc gaatctctcc catctttttg agtcgttccg
    11521 gcggaatacc ttcatcgccc gtttcttcac cgctcaatag ccacgcatct gcatttccca
    11581 ttacgaccgg gcagttcagc gcgcgtaagt tttgaacaac ttctgcaggc tgcgatcccc
    11641 cctggatcgc gtcacccaga cagacaattt gatctacact cttctctttt atgtctgcca
    11701 gtacgatttc aagggcgaag ttattgccgt gtatgtctga gataactgcg attcgcatag
    11761 atactctccc gtgtttttga tccttaagga acagaatatt tagcttagtt ggctttcttt
    11821 aagcggaatt aagtaatctt acctcaactt tattgcatgg taagatatta ggcattctgt
    11881 gaaattttgg aggtcgtgct catgcgcaag tggtccatat tcatcctgct ttggggattc
    11941 gtgctatccc cgtccacggc aggcgcacag gggggcaccc aactgcaagc ggtcaatatt
    12001 gaattatggt ctgaatatga tcagcccagc atgcttgtta ttgaagaatt tgttgtgaca
    12061 gaagatacaa cattgcccgc cgaagttact ttgcgttttc ataatgatgc acacttgatt
    12121 gccgtggcat ttactagtga gggtcagctc ttaaacgcag aatttgatgg tccgagggat
    12181 catggagatt ggcaggtctt agatgtaaac gtacgcacgc gtgacattta tcgcattgaa
    12241 tattatcagc cactgataca ggagggaaac aaacgcaaat tttcgtttga atggcttggc
    12301 gattactcag tcaagcaatt taatctgagt gcttttattc cttcagatag tgcaaatatc
    12361 gtaaccgctc cggtcctttc gagtcaaagt agtcttccga agggcttata tttgacaaac
    12421 gaagaaggct ttgggaagat acgaatgggc gaatcgtacg aattcgaaat tgaatacact
    12481 cgagactcga cgatgttgac aaacgcgacc cagggggtag atgttcaagc ctctgatcct
    12541 gtcggaccca atacgcccgg gcgcacctcc atagacaagt tgccgtggat catcggcggt
    12601 tttgggttgg cattgattgc cgtgatcttg ttcttgtttt cgtactggcg ttccatgcag
    12661 tcagagatcc atgcagcagc gactctcgca cgcacaaagc tgggccggcg gcgcggaaag
    12721 aaaggcgaaa atgttcagat ctattgccat gagtgcggta ctcgcgcaca aactggtgat
    12781 cgattctgcc gggtgtgtgg gaacaagcta cgagtcgaat aaatcgtcgt cagtaaccta
    12841 ttttctagca atccagtatt tgatttctct tcccagaggg tataacggta acaaatcgat
    12901 ccgatagcct ttcccggcat atgcttgcat cagctgtact gctttcttga gtacagcgcg
    12961 atcatattcc agggggagtt ttatcagctc ttcgatcgca gagtgtggat attttccctt
    13021 gatacggtga tacacgcgaa actccaactg ccaggcatcc aattcgccgt aaacagataa
    13081 cgcagtgagc aatccctgct gcaaatgacg ggcttcgtga atgataaggg ttttgattcg
    13141 cagatcatcc agtggcgttt cataggtata gtaatgcgag ttcaggcgga tattgccgaa
    13201 cacggtccag aatgcgccaa cgttcggacg tgccttttta atcccgattt gcgtccgacg
    13261 tgtacggata aaatctacag ccgctttgcc ttcctcgcca atttcgacga cactatccaa
    13321 cagtgaattt acccaggttg catgatccat ttctacctgc ctttaattct tagaaaccca
    13381 aactgtctcg taaagctacg gacagtaccg tgaactggat ttgtggcgtg aattttaata
    13441 cgtgaagcac ttttttgctt cttttacggg gaaatctgac atagtaaatc agtaccgctt
    13501 acagccctgt taagaccaat cgctctccaa atgcttagaa tgagattgat aactttgcaa
    13561 aggagcataa aatgttaaaa accaaatttc tttcaagcat cctactggct attaccatcc
    13621 tgttcgcgca gaccggtata gccgccgccg caccagcttt acaggacact acgccaatta
    13681 ctggaacgat tcaaacgatg agcgtggaaa ccgatgcgag tggtctgaca actgttcttg
    13741 tgactttgtt ggacgatcaa ggtgcaactc agacggtccg gttaagtgtg gaaaccgcgg
    13801 ttgaattggg attggtcgcg ctggatcctg tcactaatga gcctgtggtg gatgaaactc
    13861 aaattgggct gacggtggag gtcgatccta catctgtcat tcccgacgaa cccgttgaag
    13921 agacggctca tccaattgcc attttgctgg ctacattctt tgaggaagat gtcagcgtgg
    13981 tgaatgatta ccatgaagac ggattcggat tcggtgtcat tgcccaggca ttatggatgt
    14041 cccagaatct taacgaagat gcgtctcttg cgggtctgat cctggaggcg aaagagagtg
    14101 gtgactacag cgcatttacc ttacccgatg gttccacgcc cacgaactgg ggacagttca
    14161 aaaaggcagt attagagaag aaaaataatc ttggtgtggt tgtctcaggg caagcagaga
    14221 cagatcagga tagcacgttg accactactg ccgaagatga gcatggcaat ggcaaggaca
    14281 aggataaagg caaaaataaa aacaagaaca ataacaatcg gaataatgga aatagaccat
    14341 agtcaggcgt ttggaaatag cggagcgacg tgcattgcac gtcgctcctt ttttatttga
    14401 tagctcctga gagtctcctg cggtaaaata atttcgaggt gctatgagag aagttgcgat
    14461 tcttggaatt ggacaaacaa aaatagatga acattgggac ctatctctgc gtgagatcgg
    14521 tgggaacgcg gcgttcgcgg ccatgcagga tgcgggcatg gataaggtcg atgcgctgtt
    14581 tgtagggaat atgttatctc caatgattga cgggcagaat cagcttggag cctacttttc
    14641 ggattggatc ggcctctgga agcaggaagc tgtgaaagtg gaagctgcct gtgcctcggg
    14701 ggcggcggcc ctgcgggcag ggctcatggc cgtttcggct ggggatatcg agtcggctct
    14761 catcgtcggt gttgaaaaaa tgaccgataa ggccggccac gatatcaccg ccgcactggc
    14821 aactgcagca gatgcagatt atgagctgga gcaggggatc tccttcgttg gcattaatgc
    14881 gctgatcatg cgacgataca tgcatgagtt tggctgggcg catgcagact tcgcgccctt
    14941 ctccatcaat gcacacgcaa acgccatcca taatccgttt gcgcgcttgc atcaaaagat
    15001 cacggtagat caatttgaaa aatccagcat ggtcgccaca cccatcaact taatggatgc
    15061 ttcgccgatc ggtgatggcg cagctgcttt gatcatcgtt ccggccgaga aggtggcatc
    15121 gctggcagga aaaccacgcg tgactgtggc aggttcagcg tctgcgaccg attcgatcgc
    15181 agtccactcc cgaaaggatc cgttgtttct atctgcggcc tatcagtcag ccaaacgagc
    15241 ttatgagatg gcaagcgttg agctggatga gatcgacgta ttcgaattgc atgacgcgtt
    15301 ctcgatcatg tccgcgcttt ccctggaagc atgtggcttc gcggaacgtg gccagggggt
    15361 gagactcggt ctcgataacg agatcagccc caatggacgc gtgcctgttt gcacccgcgg
    15421 cggcttaaaa gcgcgcggtc atcccgtcgg cgcaacgggc atttatcaga ttgtggaagt
    15481 tgttcaacaa ttacgcgggg aatgcggagc gacccaagtg gatggagcca gggttggcat
    15541 ggcacagaat atcggcggtt caggcgcgac gatcattacc catatcttga aagcaaactg
    15601 agatgctttt acgtattgag gaaatagaga gaatttgcgc cgataattag taaaagcaat
    15661 accaggatgg ggagaaatgt aaatgtggtg agaatgccag ccagccgatc tcttttacgc
    15721 caatacgcaa tccatgcggc gatggcaaag aggatcggcc agattgggta cagccagacg
    15781 gcaatcacaa aattccacgc ctcctgcgta acgcccgagt caaacgccat cacagaaagt
    15841 cctgccataa gcagccaaac cactaaggat gccagtgcaa gaagttgcga gacaatcaac
    15901 cagataagaa ttggtttgcg tgaaggaagc tttgtttcca attccatgct cctgaaggat
    15961 gtttctctga gctacagagt ttggaatttt acccttattc tgcttacttt cgttgtgaag
    16021 ccatgcattt tcctatccgc aatcaaggtg tacctgcgtg ggaggaaatt ttatatcgaa
    16081 ttgtggtata agacaaaatt gcaaacgcat tttcgttgcc catgcgtcgt tgaatccctt
    16141 ttatactgtg tttgcaagga gaatatctca tgcctaattc taataaacga aaagtaggcc
    16201 gccgtgactt tacatactat atgcaagtca ccgatgatat ttcaaaacag ctgattggct
    16261 atctatcgga tatcagtacg ggcgggttta aattggatag ccagaaagag atttcgccag
    16321 ggcaggattt tcgacttcaa attgagctga ctccagatat tgcagaaaaa aactccatgg
    16381 ttttcatagc ccgaagcaag tggtgccatc gagatcatgt ggatccgaat acctttaacg
    16441 ttggatttga gattattaac atctctccca gcgatatggg tatctttcaa cggatgtttg
    16501 ataagtatgg ctcgcaaaca tcaacaagag ccaagggtgc tgatgatttt atgtggagat
    16561 agacaacaca agtcaatggt aggcgggcgg taacaccgcc cgttttttat tcccaagtct
    16621 ctgctcttat ccccaagact gcgcgccttg ttgtaaaatt cagatatgaa atttcagacc
    16681 ttacgctcca tcatccgctt ttttatgaat ctcatcaccg atatcgaagt ccacggaatt
    16741 gaaaaacttc cggcaggaaa tgtcattgcc gcagcgaatc atcttggacg gttggatact
    16801 gcggcactgc tatgtatcat tgatcgtgaa gacatcatca tgccagtggc agaaaaatat
    16861 agacatcatt tgatctatgg cgcgatgggg cgcgctgttg acgccatctg gttgaaccgc
    16921 tttgaggctg actatgcggc gctgcgacag atcctggagc ggatgaaaaa gggcgggatg
    16981 ctggtgatcg ctcctgaagg gactcgctcc aagaccgagg cactgcagga agccaaattg
    17041 ggtgtggcgt tcctggcaag caaaagcgga tatccggttc tgcctgttgc cgtaaccggt
    17101 accgaagatc gcggcatcct tgagaatttg aaacggttta ggagatcgaa gatcgtcgtc
    17161 aaagcaggcg aattgttcta tattcaagtt ccaccaggca aagggcgaga agaagcaatg
    17221 cgccaggcaa ctgatgagat tatgtgccag atcggtgtat tactcccgga aaaatatcgc
    17281 ggtgtctatg caaatcatcc acgattgaag gaattggaga gggatcatct ccctgtatag
    17341 cccgatgatc cgcgtccttc gtttcctgat ccgtattctc ctcaaactga ttgcacgatt
    17401 tgaaattcgc ggcaaagaaa atgtcccatt aacaggtggc atgatcctcg cttccaatca
    17461 catgggaatc ctggatatcc ttatggttta ctttgggatt gaccgaacgg atctgttcat
    17521 cccagttgct gaaaagtttg aaaaaatcgg ctggatccgc tggatttcca aatacttgaa
    17581 ctttctattt gttgatcgat ttaaccccga tttgaaagcc atcaggaaga tgatcgcact
    17641 catggaagag ggcaaatgcc ttgtaattgc tcccgaggga acgcgttctc tgaccggctc
    17701 gttgaatgaa ggcaaaccgg gcgtggcgta tctggcagca cgttcgggat tccctgtaat
    17761 tcccatcgcc ataacaggaa cgcctgataa agttgtcatt gaaaatgtaa agaaatttcg
    17821 caaatcacat attacactta caggtggaaa accattcgtt attcccccat taccaaacaa
    17881 agatcgagat gtagcactgc aaaaatatac tgatgagatc atgtgtcaaa ttgcagcatt
    17941 gcttccagaa caataccgcg gggtgtacgc ggatcaccct cgattgaaag aattgctttc
    18001 agaataacct aacgctgatt ctcgtcttat tcgatctgtt cgaagtttcc atccaggtgc
    18061 aggtaaaaag tttccacatt cagctgagga aatagtgcac gaatcttcgc acccgtatca
    18121 agcagatcgt gtctatgacg cttatacgtt ccttcgcgcc cataagcccg gcaatcttca
    18181 tgattgataa ggcagaccac ttcaatactg tgtatccgaa cagccagttg aacgtatttc
    18241 aaaaccatct cataatcatg cacactacca gccagtgaga tgatatcgaa gttgtcatat
    18301 ccaaaacgga cggtgatcca cggttgcaga aacttttgca ggcgatagtc catgcagtgg
    18361 acaacgaggg cgtcacaatg gattacaggt ttttttgttg tcatattcaa aggacctcgc
    18421 cgtgacctcg ttttaaaaac tgaccgccgc ccgcttttcc cagccattgc tctccgtcca
    18481 caattcgctt gccgcgtaga aaaacttttt cgggataacc tgtcagttcc cagccttcat
    18541 ataagttgta gtcggtacga tgatgggaat gtgccacgcc atatttcaca gtcttttccg
    18601 ggtcccagat cacgatatct gcatccgaac cttccatcag ggcgcctttc cgcggataca
    18661 agccaaagat cttggctggg ttcgtactca tatatgccac gaattggttt ggagtgattt
    18721 tccctgcacg gactccatat gtccatagaa ttggcattcg atcctggacg ccgggtaacc
    18781 cgtttgggat tttggtgaag tcattttttc ccaactcttt ccctggtatg gcaatctctt
    18841 ttccttcata aacgatgggg ctgcttccgt caaaaaagaa tggacaatga tctgtgccaa
    18901 tcgtctgcat cacgccgtcg gtcagacctt cccatagacg cgtgttgtct tgcctggtgc
    18961 gcatcggcgg agagcaaatc cacttggcac cgtcggcgcg gcgtaaatcc tcaattgtga
    19021 aaaataaata ttgtgggcag gtttctccca ttactttaac accgcgttcg cgagcatatt
    19081 ggagcatatc cacttcgcca cctgcgttca tgtgaacgat gtaaactggc gaatcggcct
    19141 gttctgccat ggcagccata cgaagagtcg cctcgatcgc accccaggca ggacgagtca
    19201 acgtatgata ctcgggactt gtatgtcccg cagcgattgc ttccgctacg agggtttcga
    19261 tgacatcacc gttttcgcag tgcgccatca tgagcattcc gttttctctt gcaattctca
    19321 aggctttgaa gatactgcca tcatc
//
LOCUS       DASTJP010000518         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_240306, whole genome shotgun sequence.
ACCESSION   DASTJP010000518 DASTJP010000000
VERSION     DASTJP010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240306"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..848)
                     /locus_tag="VFQ23_15265"
     CDS             complement(111..848)
                     /locus_tag="VFQ23_15265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9908009.1"
                     /translation="MITDEVWKLPEIVNRFLSYRAAIPLAQEQIGVMMSILKSRGQPV
                     ENFLDLGCGDGILGAAILGEFPNARGLFVDFSESMLEQAREQFKDFSGALAFENLDYG
                     HPAWVNQIQTYAPFDAIVSGYSIHHQPDDRKYAIYQEIFSLLKPAGWFVNIEHVASAS
                     QLNVDLFENHYVSTRYAIEQRNGGTRAFDELANEYKNRPDKAANILAPTELQCDWLRE
                     IGYEDVDCYFRIYELAVFAGRRPNLKS"
     gene            complement(1001..>2308)
                     /gene="leuC"
                     /locus_tag="VFQ23_15270"
     CDS             complement(1001..>2308)
                     /gene="leuC"
                     /locus_tag="VFQ23_15270"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET9908010.1"
                     /translation="VHEVTSPQAFTGLRQRGLKVRRPEKTLATMDHSIPTTPLNVPIA
                     DAMAAAQIRQMEVNAAEFGITLHGMDSPHRGIVHVIGPELGRTQPGMTIVCGDSHTST
                     HGAFGAIAFGIGTSEVEHVLATQCLLQTKPKTYEVRVDGKLPVGVSAKDIILALIAKI
                     GVGGGTGHVFEYTGDAIRGLSMDQRMTVCNMSIEGGARAGMIAPDDITFEYLYGREFA
                     PKGNQWDKAVAKWRSLPSDEGAAYDKSITLNAADLEPMITYGTNPGMGMRITERIPTA
                     ESFKESSQKAAFEKALTYMGLQPGQSLLGQKVDVVFIGSCTNSRISDLRLAAAFLKGR
                     KVAEGLRVMVVPGSQDVKKQAEREGLDKVFKEAGAEWREAGCSMCIAMNGDQLQPGQY
                     AVSTSNRNFEGRQGKGGRTFLASPLTAAATALTGKVTDVRTML"
BASE COUNT          552 a          606 c          566 g          584 t
ORIGIN      
        1 agaagcgggc agggatgatc tggtcagtgt ccacatcatt cactggaagt tgcacaacac
       61 gagaggtaag agtagtaaat tgagccatag gatgatttcc aattttagat ttaagatttt
      121 agattggggc ggcgtcctgc aaagaccgca agttcataga tacgaaagta gcaatcgacg
      181 tcttcgtagc cgatctctcg cagccagtcg cattgaagtt ctgtaggtgc caatatgttg
      241 gcagctttat ccgggcgatt tttgtattca ttcgccagct cgtcaaaggc acgcgtgcca
      301 ccgttccgtt gctcgattgc ataacgcgtt gagacataat ggttttcgaa caagtccaca
      361 ttaagctgcg aagcagatgc gacgtgttcg atattgacga accatccagc aggtttcaac
      421 aaggaaaata tctcctgata aatcgcgtac ttgcgatcat ctggctgatg atgaatcgaa
      481 tagcctgaaa caattgcatc aaacggagca tacgtctgaa tttgatttac ccaggctggg
      541 tgcccataat caagattctc aaaggcgagt gcaccagaaa aatctttgaa ttgttcacga
      601 gcctgctcaa gcatcgactc tgaaaaatcc acgaacagcc ctcgtgcgtt tggaaactcg
      661 cccaggattg ccgcgcccag aatcccatct ccacagccca ggtcgagaaa gttttcaaca
      721 ggctgtcctc tcgatttgag aatcgacatc atcacgccga tctgctcctg ggctaatgga
      781 atcgcggcgc gatatgacag aaagcgattc acaatttcag gtagtttcca aacttcgtca
      841 gtaatcatta tgatttttaa tcatctccgt tggttgagca ggcaacgtgg tttttcatcg
      901 ctgtatcgaa accaacaata gctataaatc tgaacgtaca gcctgtggtc tcgatatatg
      961 gctcgctatc gctcgccact actcgaccac cgctagaaaa ttagagcatt gtgcgcacgt
     1021 ctgtcacttt gccggtgagc gcagtcgcag cagcagtcaa gggagaagcg aggaaggtgc
     1081 gtccaccttt gccctggcga ccttcaaaat tgcgattgct ggttgaaact gcatattgac
     1141 cgggctgcaa ttgatctcca ttcatggcga tgcacatcga gcagccagcc tcccgccact
     1201 ctgcccctgc ttctttgaag accttatcaa gaccttcccg ttctgcttgt ttcttcacat
     1261 cctgagaacc cggcaccacc atcacacgca ggccttcagc aacctttcgg cctttaagaa
     1321 atgcagcagc gagtctcaag tctgagatgc gcgaattcgt acaactaccg ataaagacca
     1381 catccacttt ctgccccagc agagactgac cgggctgaag tcccatatag gtcaatgcct
     1441 tttcgaaggc ggctttttgt gagctttcct taaatgactc ggccgtgggg attcgctcgg
     1501 tgattctcat ccccatgcca ggattggttc cataggtgat catgggctca aggtccgcgg
     1561 cattgagcgt aatagatttg tcatatgccg cgccttcatc acttgggaga gatctccatt
     1621 ttgcgactgc tttatcccat tggttgcctt tcggggcgaa ctcacgtcca tataaatact
     1681 cgaaagtaat gtcatccggg gcaatcatgc cggcacgcgc gccgccttca atggacatgt
     1741 tgcaaacggt cattcgttgg tccatgctga gaccacggat tgcgtctccc gtgtattcaa
     1801 agacatggcc tgtccctcca ccgacgccga ttttggcgat tagcgcgagg atgatgtctt
     1861 tggccgaaac accaacagga agtttgccat ctacccgcac ttcataggtt tttggcttgg
     1921 tctgcaggag gcattgggtt gcgagcacat gttccacttc tgatgtgcca attccaaacg
     1981 cgatcgcgcc gaacgcgcca tgtgttgagg tatgactgtc accgcagaca atggtcatac
     2041 caggttgagt gcgccctaat tctggtccga taacgtgcac gatcccgcga tgcggactat
     2101 ccatcccatg cagggtaatg ccgaattcag cggcgttgac ttccatctgc ctgatttgtg
     2161 cagcagccat ggcatcggcg atcggcacgt ttagcggtgt cgttgggatg gaatgatcca
     2221 tggttgcaag tgtcttctca gggcgacgta ccttaagccc tcgctgacgc agacccgtga
     2281 acgcctgcgg tgaagtgact tcatgcac
//
LOCUS       DASTJP010000519         7930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1498036, whole genome shotgun sequence.
ACCESSION   DASTJP010000519 DASTJP010000000
VERSION     DASTJP010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498036"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VFQ23_15275"
     CDS             <1..698
                     /locus_tag="VFQ23_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET9908011.1"
                     /translation="SDILPEIGITAEVQLMRLPLLFEENLLGILWIWGKGILKADLPI
                     MSIFAKQIGISLERARLFQEVQSLAFTDPLTGLHNRRSLFEFSRVEFSRAHRLNRPIC
                     CMMLDLDHFKQINDTYGHPAGDDVLQEFARRCKSSIREIDLIGRYGGEELMIILPETN
                     LATALQIAERICDVVAEQPIKAQDGEINCTVSVGVAEKDENTTHLETLIARADQAMYI
                     AKHKGRNRVALSR"
     gene            complement(704..1723)
                     /locus_tag="VFQ23_15280"
     CDS             complement(704..1723)
                     /locus_tag="VFQ23_15280"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9908012.1"
                     /translation="MAIDTLERIQISLDEKRQNLTDFLQTASEDEKDTCLCDDEHCIE
                     EHLHVIETSLVEIQNSTLGVCEICRGYVEPSRLEMDYTAHVCLDHYSDEERRRLESEL
                     ELSQIVQRALLPQRIPDIKGVELAAFSRPSEIIGGDYFDFFNYRDGAHGLVIADVSGH
                     GVAAGMLMSSLQTALRTMAPDTEVPAEILERINRFYIHNINFTTFITVFLARFDPETR
                     ILTYINAGHNPPVVYRKETGETHWLTRTAPAIGLAEDFRPKTESITLEPGDSLLLYTD
                     GLTEAFNLKQEQFGHERLADLFVRNVNLTMPDILQVVRETVNEFSDRVPLADDATLIA
                     LKISN"
     gene            complement(1801..2526)
                     /locus_tag="VFQ23_15285"
     CDS             complement(1801..2526)
                     /locus_tag="VFQ23_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9908013.1"
                     /translation="MDSSTAARLISLNQDFYTRFGASFSATRHRIQPGVRRVLDSLNG
                     DESILDLGCGNGELARELAKRRHRGSYLGVDFSLPLLQDAESQPDGFSPIFMEVDLTR
                     LSGISKELSGNWSMITAFAVLHHIPSTELRLNLLQTVRELINHDGKFIHSNWQFLNSE
                     RLKARIQPWESASISKSDVDAGDYLLDWRDGGVGLRYVHHFDESELSELAKSAGFKIA
                     DLFYSDGEGGRLSMYQVWEPVFL"
     gene            2556..3326
                     /locus_tag="VFQ23_15290"
     CDS             2556..3326
                     /locus_tag="VFQ23_15290"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET9908014.1"
                     /translation="MLTPLEIEQLSDEFETKTPQEILTWAIDTFWPQIALSSSFQTQS
                     MPLLYMASRIRRDILVLFLDTGYHFWETLTFREQVASAWHLNVLDLYRNSRWDSFARM
                     QTRALPFADPNLCCFMHKVEPMQRALKDMLAWVSGIRRDQTTVRAKARILELQENGLI
                     KINPLLNWTKDDVQRYMQENNLPSHPLLEKGYRSIGCSPCTVAIGINDDERSGRWSKY
                     SKVECGLHTEMFKQTDFSKLKSDFNTDISPVERRRVER"
     gene            3332..4981
                     /locus_tag="VFQ23_15295"
     CDS             3332..4981
                     /locus_tag="VFQ23_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HET9908015.1"
                     /translation="MHIDRQTLWRATHLLTPDKKDLARLVLEEIRRGQDVTKSLRSHP
                     LKDGSGYLNKSMLVSIYHEMVAAGEIEADGRLLERIRMKPMRTLSGVTTVTVLTKPYP
                     CPGKCIFCPTDVRMPKSYLPDEPGAMRGLEHQFDPYAQVRSRLEQLKSVGHPTDKIEL
                     LILGGTWSSYRRDYQEWFVKRCFDAMNETLTRPSDTLSHLERGGSEGELAKVHAFNET
                     THHRNVGLVIETRPDEINPDEIRWLRHLGVTKVQMGAQSLDDHILEINKRGHDVESTR
                     QATALLRAAGFKIVLHWMPNLLGATPESDREDFARLWEDFCPDEIKIYPNQLLANAEL
                     FEYWQRGEFIPYTTQELIDLIADIKPTIPRYCRVNRVIRDIPSTNVVEGNRRTSLRQD
                     VHDEMKRRGTHCQCVRCREVRGKSVDAAMLKLDDAVYQAGGAEEHFISYVTPDDRLAG
                     FIRLSLPGKDSPQTGLIDLDRAALVREVHVYGQSLPVGVEKAGAAQHAGLGTRLLAEA
                     EQVARANGFKRIAVISAVGTRGYYLERGFERGELYLTKTLP"
     gene            5082..5963
                     /locus_tag="VFQ23_15300"
     CDS             5082..5963
                     /locus_tag="VFQ23_15300"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9908016.1"
                     /translation="MDYKDYYKILGVERKASEEDIRKAYRKLAMQYHPDRNPNDKQAE
                     ERFKEINEAYQVLSDPKKRSHYNRLGSDYSNWQRRGTPGDFDWGRYGGFPGGAGTRVD
                     VEDMDEMFGGGFSDFFRTIFGIGNTPGAQRYQQEVAITLDEAFKGTTRLLQGTNGKDK
                     QVRIPAGVRTGSKVRVAGAGPNGLDLYLIVNVQADKRFERRGNDLHTTATVNVFTAIL
                     GGEAEVETLDGKVKLNIPAGTQPEQVFRLAGRGMPSVNKESKDKGDLFVRLKVTIPKY
                     LSSKQRELLDEASRLKF"
     gene            5974..7071
                     /locus_tag="VFQ23_15305"
     CDS             5974..7071
                     /locus_tag="VFQ23_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9908017.1"
                     /translation="MKRSLFFVSILTISMLACQISGLAPLPVNTQMPDTNVQTSPPIL
                     VEAANPVPAEGTLSALYQQVLPGVVTIFTDTGLGSGFVFDGEGHVITNNHVVEGASAV
                     EVDFASGFKAHGTVIGTDADADIAVIKVDAPADQIHPLAIGDSDALLVGQSVVAIGNP
                     FGLNGTMTLGIISGLGRTQPVQAAPEGGGAFSNADIIQTDAAINPGNSGGPLFNLNGE
                     VVGVNQSIRTTNIDETTGNAANSGVGFSISINLVKRIVPGLISNGSYEYPYLGISSRE
                     LSLSEIESLGLNSYTGAFVASVVEGGPAAQAGIRAGEDLITAIDGSPVISFDDMLSYL
                     VTHKSPGDTVVLTVLRDGQPVDVTITLGTRP"
     gene            complement(7164..7808)
                     /locus_tag="VFQ23_15310"
     CDS             complement(7164..7808)
                     /locus_tag="VFQ23_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HET9908018.1"
                     /translation="MFSSFEGADRRKEEERVRNEGRLPPGQALTFKFPVLHYGPVPHF
                     NPATWDFRVWGEVEGEKRWTWDEFNKLPRAKLQMDIHCVTRWSKFDTVWEGVSVKTMV
                     ENNLFRIKPTATHVMQHAEYGFTVNLPLEVVLQENFLLATHFNGEPISPDHGYPLRGV
                     VGFIPGHEDLETPYLWKGAKWLRSLEFMTRDRRGFWEQAGYHNRADVWREERFG"
BASE COUNT         2063 a         1877 c         2036 g         1954 t
ORIGIN      
        1 catcagacat tctgccggag atcggtatta ctgctgaagt ccaattgatg cgacttcctc
       61 tgttatttga agaaaatctt ctgggaattt tatggatatg gggaaaagga attttgaaag
      121 ctgacttgcc cattatgtct atatttgcca aacagatcgg aatttcattg gaacgcgccc
      181 gtctcttcca ggaagttcaa agcctcgctt ttacagatcc tcttaccggt ctacataatc
      241 gccgcagttt gttcgagttt agcagagttg aattttcacg ggcgcatcgt ctgaacagac
      301 ccatttgttg tatgatgctg gacctggatc acttcaagca aatcaacgat acatatgggc
      361 atccggcagg cgacgacgtt ttacaggaat tcgcaagacg atgcaagagt tcaattcgtg
      421 agattgacct gattggcaga tatggcggtg aagaactcat gatcattttg cccgagacga
      481 atttagccac agccttgcaa attgccgaac gtatctgtga tgttgttgcc gaacagccaa
      541 tcaaggcgca agatggtgaa attaactgta ccgtgagtgt gggagtcgct gagaaggacg
      601 aaaacacgac tcatctggaa actttgattg cgcgtgcaga tcaggcaatg tacattgcaa
      661 agcacaaagg tcggaatcgt gtggcactaa gccgataaga tcgctagttt gaaattttca
      721 gggcaatcag ggtcgcatca tcagccagtg ggactcgatc actaaattca tttaccgtct
      781 cgcgcacaac ctgaagaata tccggcatgg ttagattcac atttcggaca aagagatccg
      841 ccagccgttc gtgaccgaat tgctcctgct tcaaattaaa cgcttcggtc agaccatctg
      901 tgtagagtaa gagagaatct cccggttcaa gggtgatgga ctcggtcttc gggcgaaaat
      961 cctctgccag cccgatggca ggcgcggttc gagtgagcca gtgagtttcg ccagtttcct
     1021 tccgatagac gaccggtggg ttatgacccg cgttgatata ggtaaggatg cgtgtttccg
     1081 ggtcaaaacg agcgagaaac accgtgataa atgttgtgaa gttgatgttg tggatataaa
     1141 aacgattgat ccgttccaga atttcggcgg gaacctctgt atctggagcc atggtccgca
     1201 gcgcagtttg caggctgctc atcagcatcc cggctgctac tccatgacca cttacatctg
     1261 caatgacgag tccatgcgca ccatcacgat aattgaaaaa atcgaagtag tcgcctccga
     1321 tgatctcgga agggcggctg aacgcagcca gttccacacc tttgatatca gggatgcgtt
     1381 gtggcaacag ggcgcgttga acgatctgag aaagttccag ctctgattcc aggcgtcgac
     1441 gttcttcatc agaatagtga tccaaacaca catgggctgt gtaatccatc tcgaggcggc
     1501 tgggttccac atatccacga cagatctcac aaacacccag ggtcgaattc tggatttcca
     1561 ccagagacgt ttctataaca tgaaggtgtt cctcgatgca gtgctcatcg tcgcataggc
     1621 acgtatcctt ttcatcttcg ctggctgttt gcaggaaatc cgtcaggttt tggcgctttt
     1681 catccaggct aatttgaatg cgctcaaggg tatcaattgc cattacttcc tccaatgcgg
     1741 tttacaccaa tatagaacat ttgtttagac tccattagtt ccgtaattct tacaaaatta
     1801 ttataagaaa acaggctccc aaacctgata catactcaac cgtccacctt caccatctga
     1861 ataaaagaga tccgcaattt taaagcctgc tgacttagcc agctccgata attcggactc
     1921 atcaaagtga tgaacgtaac gcagaccgac tcctccatct cgccaatcga gtagataatc
     1981 acctgcgtcc acatcggact ttgaaatgga cgcagattcc cagggttgga tccgcgcctt
     2041 gagcctctcg ctgttcaaga attgccagtt tgagtgaata aactttccat cgtggttgat
     2101 caactcgcgg accgtttgta gcaaatttaa tctcaactcg gttgagggga tgtgatgtaa
     2161 aactgcgaag gcagtgatca tggaccagtt accggacaac tccttactga tgccagataa
     2221 cctggtcaga tcaacttcca tgaagatagg ggagaacccg tcgggctggg attcggcatc
     2281 ttgcagcagc ggcaggctaa agtccacgcc gagatatgaa ccgcgatgtc tgcgctttgc
     2341 caactcccgg gcgagttcac cgtttccgca tcccaaatcc aaaatggact catctccgtt
     2401 gagcgaatcg agtacgcgac ggacgccggg ttgaatccgg tgacgtgtgg cagagaacga
     2461 ggcgccgaat cgggtgtaaa aatcctgatt gagactgatc aggcgcgccg cggtacttga
     2521 atccatcaaa atattataac ggttataatc ttgccatgct gacgcccctg gagatcgagc
     2581 agctctcaga tgaattcgag acgaaaaccc cacaggaaat tctcacctgg gcgatcgata
     2641 cattttggcc acagatcgcg ttgagttcat cgttccagac gcaatccatg ccgctgctgt
     2701 atatggcttc gcggatccga cgcgatattt tggtgctgtt tcttgataca ggctatcact
     2761 tctgggagac gttgaccttt cgcgagcagg ttgcttcggc ctggcacttg aatgttttgg
     2821 atttataccg taattcacgc tgggactctt tcgcacggat gcaaacacga gctctgcctt
     2881 ttgctgaccc aaacttgtgt tgtttcatgc acaaagttga acctatgcaa agagctttga
     2941 aagatatgct ggcctgggtc agtggtattc gacgtgacca gaccactgtg cgtgcgaaag
     3001 ccaggattct ggaattgcag gaaaatgggt tgattaaaat caatccgctt ttaaactgga
     3061 caaaggatga tgtgcagcga tatatgcagg agaataattt accttctcat ccattactcg
     3121 aaaaaggata tcgaagtatt ggctgttcgc cttgtacagt tgcaattggt ataaacgacg
     3181 atgaacgttc tggccgttgg tcaaagtata gtaaggttga atgtggtttg cacaccgaaa
     3241 tgttcaagca aaccgatttt tcaaagttga agagtgattt caatactgat atttctcctg
     3301 tggaacgaag acgggtagag agataaatag aatgcatatc gatcgacaaa cgctttggag
     3361 agcaacacat ctccttactc ctgataaaaa agatcttgcg cggttggtgc tggaggaaat
     3421 tcgacgcggg caggatgtga cgaagagcct gcgaagtcat ccgcttaaag atgggagtgg
     3481 ctacctcaac aagtcgatgc tggtttccat ctatcatgaa atggtcgcag ccggggagat
     3541 cgaagcagac ggacgcctgc tcgagcggat tcggatgaag ccgatgcgga cactttcggg
     3601 cgtgaccaca gtgaccgttt tgaccaaacc ctatccatgc ccgggcaagt gcatattctg
     3661 tcccaccgat gtgaggatgc ccaaatccta tctgccagat gaaccgggtg ccatgcgtgg
     3721 gttggaacat caatttgacc cttatgctca agtccgttcg cggcttgaac aattgaaatc
     3781 tgtggggcac cccacagata agatcgaatt gttaatcctc ggtggaacct ggtcctcgta
     3841 tcgacgagat tatcaggaat ggttcgttaa acggtgtttt gatgcgatga atgagaccct
     3901 cacccgtcct tcggacaccc tctcccattt ggagaggggc gggagtgagg gagaactcgc
     3961 aaaagtacac gccttcaatg agaccaccca ccaccgtaat gtgggtctgg tcatcgagac
     4021 gcgaccagat gagattaacc cggatgaaat tcgctggctg cgtcatcttg gcgtgacgaa
     4081 ggtccagatg ggtgcgcaga gcctggatga tcatattctg gagatcaaca aacgcgggca
     4141 tgatgtggaa tccacacgtc aggcgacagc gttactgcga gcagccggct tcaagattgt
     4201 attgcactgg atgccaaatc tattaggtgc cacgccagaa tcagaccgcg aagatttcgc
     4261 tcggctttgg gaagactttt gcccggacga gatcaaaatt tatcctaacc agttgctggc
     4321 gaacgcggag ctgtttgaat actggcaacg cggcgaattc ataccctata ccacacagga
     4381 actgatcgat cttatcgcgg atatcaaacc gacgatccca cggtattgtc gcgtgaacag
     4441 agtgatccgc gatataccgt ccacaaatgt ggtcgaaggg aatcgccgaa cgagcttacg
     4501 acaggacgtt catgacgaaa tgaaacgccg agggactcac tgtcaatgtg tgcgctgccg
     4561 tgaggttcgt ggaaaatccg tggatgcagc tatgcttaag cttgatgatg ctgtctatca
     4621 ggctggcggg gcggaagaac atttcatctc gtatgtaacg ccggatgatc gattagcagg
     4681 ttttattcgg ctttcgttgc caggcaaaga ctcaccgcaa acagggttaa tcgatttgga
     4741 cagagcggcc ctggttcgtg aagttcatgt ctacggacaa tcgcttccgg ttggcgtgga
     4801 gaaagcgggc gcagcgcaac atgcaggtct tggaactcgc ttgttagcag aagcagagca
     4861 agtggcgcga gccaatggat ttaagcggat agctgtcata tcagcagtgg ggacgcgggg
     4921 ttattatctc gagcgtgggt ttgaacgcgg ggaactctat ctgacaaaga cactgccgta
     4981 accaaagcaa gaagaaaaca aaatcttcat aagaattaga gttgtgttag ataaccacat
     5041 gggtgcttga cttgcatccc gcgcagtgta aaaataacac aatggactat aaggattact
     5101 ataagatttt gggcgtggag cgcaaagcca gtgaagaaga tatccgcaag gcatatcgca
     5161 aactggccat gcaatatcac ccggatcgca acccgaatga caaacaggcc gaggaacgtt
     5221 tcaaagaaat caacgaagcc tatcaggtgt tgagcgatcc taaaaaacgc tcccattaca
     5281 accgactcgg atctgattat tcgaactggc agcggcgcgg cactccgggt gattttgact
     5341 ggggacgcta tggtggattc cctgggggtg caggcacgcg cgtcgatgtg gaagatatgg
     5401 atgaaatgtt tggcggaggt ttttctgatt ttttccgcac gatattcggt atcggaaaca
     5461 cgcccggagc gcagcgttat cagcaggaag tggcgatcac cctggatgaa gcctttaagg
     5521 gaacgacacg tctgcttcaa ggcactaacg gcaaagacaa acaggtccgt attcccgccg
     5581 gggtgcgcac cggttcaaaa gtacgcgtgg ctggcgcggg ccccaacggg ctggatctgt
     5641 atttgatcgt gaatgtacag gccgataaac gattcgaacg acgaggcaat gatttgcata
     5701 caactgccac cgtcaatgtc ttcacagcca ttttgggcgg cgaagccgag gtggagacgc
     5761 tggacggcaa ggtcaaactt aatattcccg ctggcacaca acccgaacag gtattccggt
     5821 tggccggacg cggcatgcca agtgtcaaca aagaatcaaa agataaaggc gatctctttg
     5881 tacgactaaa agtaactatc ccaaaatatc tttcgtccaa acaacgtgaa ttattggatg
     5941 aggcttcacg cctcaaattc tagaaaggaa aaaatgaaac gttcgttatt tttcgtatca
     6001 attctgacaa tctccatgct ggcttgtcag atttccggac tcgcgcctct gcctgtgaat
     6061 acgcagatgc ctgataccaa tgtgcaaacc tctcctccaa tcctggtgga ggctgctaat
     6121 ccggttccag cagaaggaac cttatctgca ctatatcaac aagtgctgcc gggagtggtt
     6181 actatcttta cagatactgg actcggctca gggtttgtgt tcgatggtga aggtcatgtt
     6241 attacgaata accatgttgt ggagggtgcc agtgcagtgg aagtggattt tgcttcggga
     6301 ttcaaggctc acggcacagt catcggaaca gatgctgatg cagatatcgc agtgatcaag
     6361 gtggatgctc cggcagatca gattcatcca ttagcgatcg gcgattcaga tgcccttcta
     6421 gtggggcaat cggttgtcgc aatcggcaat ccgtttggat taaatggtac gatgacgctt
     6481 ggtattattt cagggcttgg tagaacgcaa cctgtacaag cggcgcccga gggtggtgga
     6541 gcatttagca atgcagatat catccagaca gacgcagcga ttaatcccgg taactcaggc
     6601 ggtccactct tcaatttgaa tggtgaggtc gtgggagtga atcaatcgat tcgtacaacg
     6661 aacatcgatg aaacgaccgg gaatgcggct aattctggtg ttgggttttc catctcgatc
     6721 aatttggtca aacggatcgt gcccggtctg atcagcaacg gaagctacga atatccatat
     6781 ctcgggattt cttccagaga gttgagtctt tcggagattg aatcccttgg cttaaatagt
     6841 tatacgggtg catttgtcgc cagtgtcgtg gaaggtgggc cagcggcgca agctggtatc
     6901 cgtgccgggg aagatttgat cacagcgatc gatggtagtc cggtgatctc attcgatgac
     6961 atgttaagtt atctggtcac acataaatcc ccgggagata ctgtagtatt gacggtgctg
     7021 cgtgatggtc aacctgttga tgtcacgatt accctgggca cccgccctta aaaaaaatgg
     7081 cgtctcactt tgcagatccg ccgtctcaac ttgatcataa tgagggcagc atgttttgca
     7141 acactgctgc ccttccattt tttctaaccg aatctttctt cacgccacac atccgcccga
     7201 ttatgataac cagcctgctc ccagaaacct cgtcgatcgc gagtcataaa ctcgagcgag
     7261 cgtagccact tggcaccttt ccataaataa ggcgtttcca gatcttcgtg accggggatg
     7321 aaacccacta ccccgcgcaa tgggtaacca tggtcgggcg aaattggttc tccattaaaa
     7381 tgagtcgcga gcagaaagtt ttcctggagc acaacctcga gtggaaggtt tactgtaaag
     7441 ccatattctg catgctgcat cacatgagtg gcagtcggct taatcctgaa gaggttgttc
     7501 tccaccatgg tcttgaccga aacaccttcc cagacggtat caaacttact ccaccgtgtg
     7561 acgcaatgga tgtccatttg aagctttgcc cgaggaagtt tgttgaattc atcccatgtc
     7621 cagcgtttct ctccctccac ttctccccag acgcggaagt cccaggtagc ggggttgaaa
     7681 tggggcacgg gcccatagtg aaggacagga aacttaaagg taagcgcctg cccgggtggc
     7741 aaacgtccct cgttacgaac tcgttcttcc tctttacggc ggtcagcacc ttcgaatgat
     7801 gaaaacacgg caactcctct cgaaaatttc aatttctgta cagaatagac tcttggggaa
     7861 tgcgatttgt tacaatgatt cagtaatcat taatactttt gtcagaaatg caccctttcc
     7921 atgagcagca
//
LOCUS       DASTJP010000520         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_779802, whole genome shotgun sequence.
ACCESSION   DASTJP010000520 DASTJP010000000
VERSION     DASTJP010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779802"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..904
                     /locus_tag="VFQ23_15315"
     CDS             161..904
                     /locus_tag="VFQ23_15315"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9908019.1"
                     /translation="MTEIAMQELRQAITRGTFRPGTQLPTEADLCQMLGVSRTVVREA
                     LRVLEDDGLVSRRHGVGTFVRDHPILKNLNFNFGITEMIESAGLTSGTTQLAVQTETA
                     DAETAEQLRVGLGTPLVTIKRVRTADGRPVVYSLDTLPEALLQRADFDPQLLLTQSIY
                     SLLQTTLGQVIEYGIARLMSVNAPPDVAEKLLLQPNAPTLYIVQTDYSPNDEPLVYSQ
                     EYHLPDAFDFVIWRRGPTRLQPSSSEAEQ"
     gene            901..1731
                     /locus_tag="VFQ23_15320"
     CDS             901..1731
                     /locus_tag="VFQ23_15320"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET9908020.1"
                     /translation="MNPITFSTLACPDWSVETVIAKTSEYGYDGIEWRGGSDGHIPPT
                     ISESQIAALQRTSANAGLFAVAVTAYTSFVSNLASERQTSIDELRRYSDFAAELDAPY
                     VRAFLGELPQGTLLDSSLYENISDDLNTAAAYAESVGVKIAIEPHDDFVRSSTIVPIL
                     NQVQHAALRVIWDLGNTFAADENPTEGFEILKDRIAYVQVKDGIKDHTTWKLCPLGQG
                     NVPLDQAIRLLLANDYQGAFSVEWEYAWHRELDPPEVALPAALKTVRELLSAAQTESA
                     "
     gene            1728..>2839
                     /locus_tag="VFQ23_15325"
     CDS             1728..>2839
                     /locus_tag="VFQ23_15325"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HET9908021.1"
                     /translation="MTVGRTYASEWRDFRDPLTGRRVRQLTDSSAEDYHLYFYNTSVT
                     SDGKYLIFISERTGVSNLFRLDLERGEIVQLTEARPVRAEYWPFTESIKGVGACLPAI
                     GNHGREAFYFEGTDLFAVDVESLKQRQLLSLPADRRPSMLQADATGETLVFATWDESL
                     FMERSQRAYDGEKFPDDQFFQETTSTIMRLDTATGKAEEIFCKEKFWINHVHVNPHNR
                     DLILFCHEYSSMPDRMWLLNVANGQYAPIPGQGVDEWYEHEFWSHDGQRVCFHGGSSR
                     DSTQGFCGWCSPDGSDYIRAYHSTSGRAYAHYNLHPDGQTMVTDGEARPGCISKVHLQ
                     DGQQVFEILCRHDSYTFGDDQRCHPHPSFTPDGNQV"
BASE COUNT          706 a          701 c          721 g          711 t
ORIGIN      
        1 gtggaagttc agaattctta gtttgctaca cgaagtattc ctgtaaaatt agccctaacc
       61 ctttactcag ttttcgattt gtagtactat cagtcatcat gacatcttac aactgattcg
      121 gagcgcaatg ctgaaaccag ccagggtaga aagaaaaaca ttgactgaaa ttgcaatgca
      181 ggagctgcgc caggcaatta cccgcggcac gtttcggccc ggtactcaac ttcccactga
      241 agctgacttg tgccaaatgt taggggtgtc ccggactgtt gtgcgtgaag ccctccgtgt
      301 tttggaagat gatggcttag tctctcgccg tcatggtgtg ggcactttcg ttcgagacca
      361 tccaattctc aagaatctta atttcaactt cggtattact gagatgatcg aatcagcagg
      421 gctgacttcg ggcaccacgc agcttgcggt tcaaactgaa acagccgatg cggaaacagc
      481 ggaacaactg cgcgttggcc tgggtacccc acttgtgacg atcaaacgag tgcgcacggc
      541 cgatggacgc ccggttgtct attcgctaga cacacttccc gaggctttgc ttcagcgagc
      601 ggatttcgat ccgcagcttt tgctgaccca atccatttac agcttattac agaccactct
      661 gggacaagtc attgaatatg gcatcgccag gctgatgtct gtcaatgcac ctccggatgt
      721 agcagaaaaa ctgctgcttc aacccaatgc ccccaccctc tatatcgtcc agactgacta
      781 ttcacccaat gatgagcctt tggtgtattc acaggaatat catttaccag acgcctttga
      841 ttttgtaatt tggcgacgtg gcccaaccag attgcagcca tcctcaagcg aggcggaaca
      901 gtgaacccaa tcacgttcag cacactcgcc tgcccagatt ggtctgtgga gacggtcata
      961 gcaaagacct ctgaatacgg ctatgatgga attgagtggc gagggggttc agatgggcac
     1021 atccccccta caatctcgga atcccaaata gcagctttgc aaaggacctc tgcaaatgca
     1081 gggcttttcg ctgtagctgt cactgcttat acgagttttg tttccaatct ggcaagtgaa
     1141 cgacagacaa gcatagacga actgcggcgc tattcagatt tcgctgccga actggatgca
     1201 ccgtatgttc gagcgttttt gggggaactg ccgcagggaa cactccttga ttcgtctctg
     1261 tacgaaaaca tctcagacga tttgaacaca gcagcggcat atgcggaatc ggttggggta
     1321 aagattgcca tcgagcctca tgacgatttt gttcgctcat ctaccattgt gccgattctc
     1381 aatcaggtac aacatgctgc ccttcgcgta atttgggatc ttggcaatac ctttgctgcc
     1441 gatgaaaatc cgaccgaagg gtttgaaatc ttgaaagacc gcattgcata cgtgcaagtg
     1501 aaggatggaa taaaggacca taccacgtgg aaactctgcc cattaggcca gggcaatgtt
     1561 cctctggatc aggcgattag attattactg gcaaacgatt atcaaggggc attcagcgtg
     1621 gaatgggagt atgcctggca ccgtgaattg gatccaccag aggttgcctt gccggccgcg
     1681 ctgaaaacag ttagagagct attatctgct gcacaaacgg agtcagcatg acggtcggca
     1741 ggacctacgc gtctgagtgg cgcgactttc gtgatccgct gactggtcgt cgcgtgcgac
     1801 aactgaccga ttcgtccgcc gaagactatc acctttattt ttataatacg tcagttacat
     1861 cggatggcaa atacttaatt ttcatttctg agcggaccgg agtgagcaat ctctttcggt
     1921 tagatcttga gcgcggagaa attgtgcaat taacggaagc acgtcccgtt cgcgcggaat
     1981 actggccctt cacagagtcg attaaaggcg ttggcgcatg cttacctgca atcggcaatc
     2041 atggacggga agccttctat tttgaaggaa cagatttgtt tgcagttgat gttgaaagtt
     2101 tgaaacaaag acaattattg agcttgcccg ccgaccggcg tccatcgatg cttcaggcag
     2161 atgccacggg tgaaaccctg gtcttcgcaa catgggatga gagcctattc atggagcgct
     2221 cacagcgcgc ttacgacgga gagaagttcc cggacgacca attctttcag gaaacaacca
     2281 gcaccattat gcgtctggat accgccacag gaaaagcgga agaaatattt tgcaaagaga
     2341 agttttggat caaccatgtg catgtcaatc cacacaacag agacttgatt ttgttctgtc
     2401 atgagtacag ttcaatgcca gatcgaatgt ggcttctgaa tgtcgcgaac gggcagtatg
     2461 cacctattcc aggccagggc gtggacgaat ggtatgaaca cgaattttgg agtcacgatg
     2521 gtcagcgcgt ttgttttcat ggtggatcat cgcgtgatag tacgcaggga ttttgcggat
     2581 ggtgttctcc cgatggttca gactacatcc gtgcctatca ttcgacttcg ggccgcgcgt
     2641 acgcacatta caacttacac cctgatgggc aaaccatggt cacggacggt gaggcgcgcc
     2701 cgggctgtat ttccaaggtg catctgcaag atggacaaca ggtttttgag attctttgca
     2761 ggcatgactc ttataccttc ggggatgatc aacgctgcca cccccatccc tcgtttacgc
     2821 ctgatggtaa ccaggtgat
//
LOCUS       DASTJP010000521         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_723126, whole genome shotgun sequence.
ACCESSION   DASTJP010000521 DASTJP010000000
VERSION     DASTJP010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723126"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFQ23_15330"
     CDS             complement(<1..677)
                     /locus_tag="VFQ23_15330"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9908022.1"
                     /translation="MNTKTFLQSFILITLLLALFASTSPALAASCGTSVTVASGDTVR
                     KIAERCVTTVSALRRANPEIGSGDLIYPGQILLLPGAIINGNNGYNTYIVARGDTLKS
                     LANRFGTSIDTLVSLNSSIVNVNLIYEGQRLVVPSGQGVPVPPPPTGGQVYYAQSGDT
                     LRKIAAKFNTTVDAIVRANPQISNPNVIYVGQAITIPAQASTHVVQKGDTLRIIAARY
                     GTTVDALL"
     gene            912..>2191
                     /gene="eno"
                     /locus_tag="VFQ23_15335"
     CDS             912..>2191
                     /gene="eno"
                     /locus_tag="VFQ23_15335"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9908023.1"
                     /translation="MDTVIESISALEILDSRGNPTVEVEVTLADGSWGRAAVPSGAST
                     GEHEALEMRDGNKKRYLGKGVTKAVANVNGVIADALFGWDASEQKAIDMELLAIDGTP
                     NKSKLGANAILGVSLAVAKAAANSFGQPLYRYLGGVYAHVLPVPMLNIMNGGAHTGWQ
                     STDMQEFMVMPFGAGSFSEGLRWGAEIYHTLKSVLKEKGYPTTVGDEGGYAPSLKANN
                     EAVELILSAIEKAGFKAGRGQDVAIALDPAASELYEEESKTYNLRKEGKKLTGEQMVD
                     FWAKWINDYPIVSIEDGLAQDDWDAWKLMVQKVGNKCQLVGDDLLVTNAERVRRAIKE
                     NAANALLVKVNQIGSLTETIEAVETCQRAGWRAVTSHRSGETEDTTIADIAVALNSGQ
                     IKTGAPARSDRVAKYNQLLRIEAELGETAKYAGWG"
BASE COUNT          570 a          507 c          626 g          488 t
ORIGIN      
        1 agcagggcat ctacagtagt tccatatcgt gcagcgatga ttctgagggt atcgcctttt
       61 tggacaacat gggtggaggc ctgtgcgggg attgtgatgg cctgtccgac gtagatgaca
      121 ttcgggttcg agatctgcgg gttggctctg acaatcgcat cgacagtcgt gttgaacttc
      181 gccgcaatct tgcgcagggt atcaccactc tgagcgtaat agacctgtcc accagttggc
      241 ggaggcggca cgggcacacc ctgccctgag ggcacgacca agcgttggcc ttcgtagatc
      301 agattgacat tgacaatgct gctgttcaga gaaactaggg tgtcaatcga ggttccgaaa
      361 cgatttgcca gcgatttcaa cgtatcacca cgcgctacaa tataagtgtt gtagccattg
      421 ttcccgttga tgatagcacc cggcagcaac aggatttgcc cggggtagat caaatcaccc
      481 gaaccgatct cagggttggc tctccgcagg gcggaaacgg ttgttacaca gcgctcagcg
      541 atcttgcgca cggtatcgcc gctggcaact gtgacgctcg tgccacagct ggcagctaat
      601 gctgggcttg tcgaagcgaa cagggcaagc aacagtgtaa taaggataaa ggactgtaaa
      661 aaagtttttg tgttcatcga aatctccatt tcttgatgtt ccatggagta gatgaatttc
      721 catatttaaa agttcccaaa aggcggcagg cagtactaac ttcctatagt tttcgtcgga
      781 tcaccggatg gagaaatcgg taagtgcaat ttgggctgag tctggaggca aattgaaagt
      841 ggattatact taccgtcgct tgctgaggtg gcttaagcaa gacgtgaaat tatctgacac
      901 tggagaatga aatggacaca gttattgaaa gcatctctgc gctggaaatt ctggattcac
      961 gcggcaaccc gacggtcgaa gtagaagtga cgctggcaga tggctcgtgg ggacgagcag
     1021 ccgttccatc gggcgcatcc actggcgaac atgaagcgct ggaaatgcgc gacggcaaca
     1081 agaaacgata ccttggcaaa ggcgtgacca aggcagtcgc caacgtcaac ggcgtgattg
     1141 cagatgccct ttttggttgg gatgcctccg aacaaaaagc aattgacatg gagcttctgg
     1201 cgatcgatgg cacgcccaac aagtcgaaac tcggggcgaa tgcgatctta ggggtgagtc
     1261 tggcggtcgc aaaggcagcc gcaaattcgt ttggtcaacc gctttatcgg tatctgggtg
     1321 gtgtatacgc gcacgttcta ccagtaccca tgcttaatat tatgaacggc ggtgcacata
     1381 ccggctggca atccactgac atgcaggaat tcatggtcat gccgttcgga gcggggtcat
     1441 tttcagaagg ccttcgttgg ggtgcagaaa tttatcatac gttgaagtcc gtactgaagg
     1501 aaaaaggcta tcctacaaca gtaggcgatg aaggcggata cgcaccatcg ctgaaggcga
     1561 acaacgaagc cgttgaattg atcctcagcg caatcgaaaa ggcaggcttc aaagcaggtc
     1621 ggggtcagga tgttgcgatt gctctcgacc ccgcggcttc tgaactttat gaggaagaat
     1681 caaaaaccta caaccttcgc aaggaaggca agaaattaac cggtgagcaa atggtagatt
     1741 tctgggcaaa gtggatcaac gattacccta tcgtttccat cgaagatggc cttgcacagg
     1801 acgattggga tgcatggaag ttaatggtcc aaaaagttgg aaacaaatgc cagctggtcg
     1861 gtgatgatct tctggtcacc aacgccgaac gggttcgccg ggcgatcaaa gaaaacgcgg
     1921 cgaatgccct cctggtgaag gtaaatcaaa ttggttctct cactgagaca attgaagccg
     1981 tggaaacctg ccagcgggcg ggatggcgag ccgtcaccag tcatcgctcc ggtgagaccg
     2041 aagatacaac cattgccgat atcgccgttg cacttaattc aggacagatc aaaacaggtg
     2101 cacctgcgcg gtcagatcgg gttgcgaaat ataatcagtt gcttaggata gaggctgaac
     2161 tcggtgagac tgccaagtat gcaggctggg g
//
LOCUS       DASTJP010000522         3618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_215134, whole genome shotgun sequence.
ACCESSION   DASTJP010000522 DASTJP010000000
VERSION     DASTJP010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215134"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFQ23_15340"
     CDS             complement(<1..504)
                     /locus_tag="VFQ23_15340"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908024.1"
                     /translation="MSTSTYRLPSHVSPRHYDIQLDAQVGRESFTGTVTIALDIHESR
                     DTIDLHARDLNINHAELNIDGESRTAVISLDPDNERIALQFSQAFPIGEASLKLSFGG
                     KVSQNLKGLYLAQNRPEQVLCTQCEATDARAIFPCFDEPTFKASFAFEITTPDPVVLA
                     NGPLLSVT"
     gene            complement(677..3472)
                     /locus_tag="VFQ23_15345"
     CDS             complement(677..3472)
                     /locus_tag="VFQ23_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9908025.1"
                     /translation="MASLPTGTVTFLFTDIEGSTKLAQEHPEHWESMRERHDAILKSA
                     IESYEGHIFQTTGDGVCAGFHTAKDAVCSVIQSQLDLAHEDWGESAVKVRMGIHTGKA
                     DIQESGEYIGYLTLSRVQRLMSAGHGGQVLVSLATQELIRDDLPEAATLRDLGERRLR
                     DMSHAEHIYQLDIATLPVEFPPIKSIDAALHNIPAPMTSFIGREKELAEIKRTLSENR
                     LVTLTGSGGAGKTRLSLEVGSQSLNEYPNGVWLAEFADVTDPGLVPQTLLSIFNLRED
                     SHRSVSEVLIDYLRAKTTLLLFDNCEHLIDACARISESLLQACPKLRILASSREALGI
                     PGEAAYRVPSLTTPDPAELPSLDVLENLDSIRLFIERAAATKPGFQLTNDNASFVAQI
                     CSRLDGIPLALELAASRAKVLTPEQIASRLDDRFRLLTGGSRTALPRQQTLRAMIDWS
                     YSLLTEQEKTLFRRLAVFVGGWTLEAAELVCADDEQDGILPHDILDLLTRLVDKSLVT
                     IEDVMGETRYHRLETIRQYSREKFFESNEVELIRNWHLNFFVRFAEQVDENLKGRDQV
                     LWQNRMATEQDNLRAALEWALNKNPDAALRIAGASNLFWTAGGYSAEGFRWTQKALEL
                     TEKPPISEGISHEQRLAARARALRGLTRLYLSLGENANAKRAAEESVELYRQSTDRRG
                     LAFALVILAYPLEFLGERERAEAVLHESYDIARAEKDVYVICRSLNILARIVSYLYKD
                     HDLARSYVEESYRLSKEAGLRSQEAQSSEILGFIASDGKDYDTARSYFKESARVYEEI
                     NASFNVILEKSNLGHLERRTGNYERALEYYRETILAFRDMGQTGAVAHQLECFGFIAF
                     AQSQNDRAVQLFSAANALREKAGTPMRPDEQSYFDKQLEALREKMGTSQFDALWSEGH
                     IITTERAIELALEVDNA"
BASE COUNT          892 a          925 c          896 g          905 t
ORIGIN      
        1 ggtcacggaa aggagtggtc cattggcaag cacaacggga tcaggtgtgg taatttcaaa
       61 ggcaaagctg gctttgaaag ttggctcatc aaaacacgga aagatagcgc gcgcatccgt
      121 ggcttcacat tgcgtacaga gcacctgttc gggtcgattc tgtgctaaat aaagtccttt
      181 gaggttctgg ctcaccttac ctccaaagga tagctttaat gaagcttcac caatggggaa
      241 tgcctgagaa aattggagag caattctttc gttgtctggg tcaagcgaga tgaccgccgt
      301 gcggctttcg ccatcgatgt tgagttctgc atggttgata ttaagatctc tggcatgcag
      361 gtcaattgtg tctcgcgatt catggatatc cagcgcgatg gtcacagttc ctgtgaagga
      421 ctcgcgcccc acttgtgcat ccaattgaat atcataatgg cgcggcgaaa catgagaagg
      481 aaggcgatag gtagacgtgc tcatttaatt tctcctgcga gataatgatg tatgtgactt
      541 ggtaagacct tgatgcaccg acattgtttc tccctgtatc catcaattac gtttttagta
      601 tccgttcatg agtgtagatt gcaagtttca gaaggaattc acaaaaaagg aaaaacgact
      661 tgaacacaag aaaacatcat gcattgtcta cttccagtgc caattcgatc gctcgttctg
      721 ttgtaattat atgaccttct gaccataacg catcaaattg actggtaccc attttctcgc
      781 gtaaggcttc caactgtttg tcgaaataac tttgctcatc cggtctcatc ggcgtgccgg
      841 ctttttctcg cagggcattc gccgcggaaa ataattgcac tgcgcggtca ttctggcttt
      901 gcgcgaaggc aataaacccg aaacattcca actggtgggc gaccgcgcca gtttgcccca
      961 tatctcgaaa ggcgaggatc gtctcccgat aatattccag cgcgcgctca taattacctg
     1021 ttctgcgttc cagatgacca agattgctct tttcgaggat cacattgaat gacgcgttga
     1081 tctcttcata gactctggca gactctttga aataggatcg cgccgtatcg tagtcctttc
     1141 catcgctggc aatgaagccc aggatttcag aggactgtgc ttcctgcgaa cgaagacctg
     1201 cttcctttga aagacgataa gattcctcta cataactccg tgcgagatca tgatccttat
     1261 acaagtagga aacaatgcgc gccaggatgt tcaacgaccg gcagatcaca tagacatcct
     1321 tttctgcacg ggcaatgtca tagctttcgt gcaggactgc ttccgcgcgc tcacgctctc
     1381 ctaaaaattc gagagggtat gccaggatca ctaaggcaaa tgccaagcct cgtcgatcgg
     1441 tgctttggcg atacaactca acgctttctt ccgcggcgcg ctttgcgttt gcgttctctc
     1501 ccaaagacaa atatagcctc gtcaacccgc gcagcgccct ggcacgcgct gccaaacgct
     1561 gttcatggga tatgccttca gagataggag gtttctctgt taactccagg gctttctgtg
     1621 tccagcggaa accttctgca gagtagccgc ctgccgtcca aaagagattt gatgctccgg
     1681 cgattcgcag ggcggcatcc ggatttttat tcagggccca ttccagcgca gcacggaggt
     1741 tatcctgctc ggtagccatt ctgttctgcc agaggacctg atctctgcct ttgagatttt
     1801 catcgacttg ctcggcaaat cgtacaaaga aattgagatg ccagttgcga attaattcaa
     1861 cttcattgga ctcaaaaaat ttctcacggg aatactgtcg aatggtttca aggcgatgat
     1921 agcgagtctc gcccataaca tcttctatcg tcaccagcga tttatcaaca agacgtgtca
     1981 gtagatcgag gatgtcatga ggtagaatgc catcctgttc atcgtctgca cagaccaatt
     2041 ccgccgcttc gagcgtccag ccaccgacaa agacagccag cctgcggaac aaggtcttct
     2101 cctgttccgt gagcaggctg taactccaat cgatcatggc gcgcagagtt tgttgccgcg
     2161 ggagcgcagt gcgcgagccg ccagtcaata aacggaagcg gtcatcgagt cgtgatgcga
     2221 tctgttcagg cgttagaact ttggcgcgcg aagcagccaa ttccaacgcg agagggatgc
     2281 catccaaacg ggagcagatc tgagcgacga acgaagcgtt gtcatttgtc aactgaaaac
     2341 ccggtttggt agcggcggct ctttcaatga agagacgaat tgaatccaag ttctccaaca
     2401 catccaggga aggtaactct gccgggtcag gagtagttaa ggaaggcaca cggtaggcag
     2461 cttcacctgg aatccctagc gcctcgcgac tgctcgcaag gatgcgtagt ttggggcagg
     2521 cttgcaacag cgactcgctg attcgcgcac aggcatcgat gagatgttca caattatcga
     2581 aaaggagcag tgtggttttt gcgcgcagat aatcgatcag cacttcagat acactgcgat
     2641 ggctgtcctc gcgcaaattg aaaatggaga gcaatgtttg tggcaccagt cccgggtcag
     2701 tcacatccgc gaattccgct aaccatacac cgttaggata ttcattcaag gactgtgaac
     2761 ccacctctaa gctaaggcgc gtctttcccg cgccgcccga gcctgttagc gtcaccagac
     2821 gattctcgct cagggtccgc ttgatctcag ccagttcttt ttcacgccct atgaaactgg
     2881 tcatcggcgc agggatgtta tgcagagctg catcgattga tttgatggga ggaaattcaa
     2941 ccggcagagt agcgatgtca agctgataaa tgtgctctgc gtgactcata tccctcaatc
     3001 gccgttcgcc caggtctcgc aatgtggcgg cttcaggcag gtcatcgcgg atgagctcct
     3061 gagtagctag tgagactaac acctgccctc catgccccgc cgacatcagg cgctggacac
     3121 ggctcaacgt aagatatcca atgtattcgc cgctttcctg gatatctgct tttccagtat
     3181 ggatccccat ccgcactttg accgcagact ctccccaatc ctcgtgggct aaatcgagtt
     3241 gagactgaat cactgaacaa actgcatcct ttgcggtatg gaaacctgca caaaccccat
     3301 cccctgtagt ttgaaagata tgaccttcat acgattcaat tgcagacttc aaaatcgcat
     3361 catgtcgctc ccgcatggat tcccagtgct caggatgctc ttgagccagt ttggtgctgc
     3421 cttcgatatc tgtaaagagg aaagtaacgg tgccggttgg caagcttgcc ataaagtcag
     3481 tatacagggt gtatgaaata ctttcaagaa gacatccctc cattttgtgc tgaagggagt
     3541 gcaaaatgag gggacaagga agggcgcttg ccttttcgtc tcaaaaacta tacaatgatt
     3601 caagtcatcg gaggactc
//
LOCUS       DASTJP010000523         6536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1075809, whole genome shotgun sequence.
ACCESSION   DASTJP010000523 DASTJP010000000
VERSION     DASTJP010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075809"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFQ23_15350"
     CDS             <1..244
                     /locus_tag="VFQ23_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET9908026.1"
                     /translation="VGNIAQGAVPPYVGPTSLYVLPGYQFRVVDAIITNFHLPKSTLL
                     MLVSAFVGREKILETYKTAVEEGYRFYSFGDAMLIL"
     gene            complement(247..528)
                     /locus_tag="VFQ23_15355"
     CDS             complement(247..528)
                     /locus_tag="VFQ23_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908027.1"
                     /translation="MKNIVFVIKDERHAEIQEGVFEQLSDVLDELERRATIAWDERPN
                     RAPCPNWEKCGRNYEIVKYDQSSVPWMELRKTAALEISANGINWLLPPT"
     gene            complement(525..1607)
                     /locus_tag="VFQ23_15360"
     CDS             complement(525..1607)
                     /locus_tag="VFQ23_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET9908028.1"
                     /translation="MPRTIKVATVQMDAMPAPLTERLARAEKIITKAAQAGAQLVVLP
                     ELFNTGYAYSDDNFERAETLEGLTSTWMKTLSERVSIHLAGAILLLDGGEIYDTMLLF
                     SPSGQRWRYDKNYPWAWERAYFRGRRGMTVAKTELGDLGLMICWDVGHVDIWKQYAGK
                     ADMIVISSCPPDGSAASFEFPNNEKLEMNDLETMSSIKDVGKTFFGQMVDQQAKWLGI
                     PAVNSGASGQVRTHLPRARALLTSLALFAPRILNFLSQAKSLEMSCEMIPSCKVVGAS
                     GNILAARTPNEGEGFVISEVTLTDSKPVPATKQPYPPLPRSTARMAFFNADVMVPFLM
                     RSVYKNGLKKVKAITHQASAQSIFSK"
     gene            1714..2421
                     /locus_tag="VFQ23_15365"
     CDS             1714..2421
                     /locus_tag="VFQ23_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9908029.1"
                     /translation="MADSLRALNQEIIACRKCPRLVAWREEVARVKRKAYRDQEYWGK
                     PVPGFGDSNARVLVVGLAPGAHGSNRTGRAFTGDASGGFLYPALYRAGFANQPTAVSR
                     ADGLVLNDLYIVASARCVPPDNKPTPEELSNCQPYLERDLQILKPKVIVCLGRIAFER
                     ILKMFSVRNSSWKFGHGATYRMDDGVWILCSYHPSQQNTLTGKLTVTMFDEIWAKARD
                     LLQAEPEVIDTANKASV"
     gene            2424..3458
                     /locus_tag="VFQ23_15370"
     CDS             2424..3458
                     /locus_tag="VFQ23_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908030.1"
                     /translation="MPLGQIAFLGSGETSLAGGRIFEALARKINEPLRIALMETPAGF
                     ELNSSQVVGKVGDFMAIRLQNYKPIIDIVAARKKDSPYSPDDPEIIKPLLYANMIFMG
                     PGSPTYAIRQLKDTLAWDVIRARHRLGATLIFASAATISIGAHSLPVYEIYKVGQDVH
                     AVDGLDLFKDFGLHLSLIPHWNNADGGVDLDTSRCFVGMDRFAEWCNLIPSENTTLGL
                     DEHTGIMIDFTNGICEVSGVSSVSLVRKCDPEIYSSGSKFALSELGEISIPDPVEKDI
                     PADVWDMVINVPSLQDDSPSDEVVALAEQRVSARETKNWPESDKLRDQISALGWTVQD
                     SKDGYKLVRN"
     gene            3549..4844
                     /locus_tag="VFQ23_15375"
     CDS             3549..4844
                     /locus_tag="VFQ23_15375"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET9908031.1"
                     /translation="MRAADVIIKKRDKGELSREEIEFFINGFVSGEVPDYQASAFAMA
                     VLLNGMTARETTDLTLSMAHSGQMLDLSDVVDLAVDKHSSGGVGDKTSIAVLPIVAAC
                     GLPVGKMSGRGLGFSGGTLDKLESIPGYRVDLSTDEFKQQLREKGIVLTGQSLDLAPA
                     DGKLYALRDVTGTVQSIPLIASSIMCKKLAAGAQAILLDVKVGNGAFMETLDEARELA
                     NLMVDIGKLAGREVIALLSDMNQPLGNAVGNSLEVIEAIDMLKGQGPEDFREHCLHVC
                     EHLLVLGKRAKDLDEGRALAEKSIADGSATEKLRVLVRAQGGDVSFVDDTSKFPRAEY
                     IEVVESPQDGYLTHILARTVGETSVTLGAGRAKKSDPVNHAVGFLIHHKVGDKVAKGE
                     PLFTIHANDKAKSAEVRELVLAAHQFSDTPVAPLPLFYD"
     gene            4918..>6536
                     /locus_tag="VFQ23_15380"
     CDS             4918..>6536
                     /locus_tag="VFQ23_15380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9908032.1"
                     /translation="MAKVPLRAYNREIETMIDRGQLDEAIAHCHHIFKTFPKHLETYR
                     LLGKAYLEYKRYKEAVDVFSRVLASEPNDFVANVGMSIIRDEENKLDDAIWHMERAFE
                     TQPSNAAIQSELQRLYGRRDGVQPPRIRMTRGALAHMYVQGELYPQAISEIKSVLKED
                     PGRSDMETLLARACYRSGLKNEAADAASRVLRRYPYCLDANRVLMEIFSTDKPDNAQI
                     YRQRVFELDPYAAQVTGTVFQSNEVPDAAISLEHLDWNGQPVGTGAQAAWSENQAISL
                     ESGVSRDEQPEWLRNAYADTTPPTTPSPTRPIDPTTGMPVPAFDTSAQPGSKDDIPDF
                     LRAAGWGQSTGAFDESKPIFGTDEPAAPPLEQGEIPDWVKAMAPSETTESTPAADEEL
                     PDWINKIGTNSLPVPTPSDSGQVDWMSQLGQTSEEASSPQTTGSDLDWMNQLGQASQS
                     MTGESSVNDEPDWMKSSDQPESALSSAAADTAPDWMNQPDQPQQAASMEQSSDDQLDW
                     LKQLDQPESSASDPASEELPEWMKTSDQPMQS"
BASE COUNT         1598 a         1597 c         1712 g         1629 t
ORIGIN      
        1 agttgggaat atcgcgcagg gggccgtgcc accctatgtt ggcccgactt ctctttacgt
       61 cttacccggt taccaattca gagttgtgga tgcgataatt actaattttc atcttccaaa
      121 atcaacgttg ctgatgttag tcagcgcttt tgtgggaaga gaaaagatcc tcgaaaccta
      181 taagaccgcg gtcgaagaag gctatcgctt ttattcgttt ggcgatgcga tgctaatttt
      241 atgatgttat gtgggtggga gtaaccagtt tatgccattg gcacttattt ccagcgcggc
      301 ggtttttcga agttccatcc atggcaccga agattgatca tatttcacga tttcatagtt
      361 gcgaccacat ttttcccagt tgggacaggg agctctgttt ggtctttcgt cccaggcaat
      421 agtagcccgg cgttctaatt cgtcaagtac gtcagaaagc tgctcaaaaa cgccctcttg
      481 aatttcagca tgccgctcat ctttgataac gaacacaata ttcttcattt tgaaaatatg
      541 gattgagcag aagcctgatg ggtaatggct ttgactttct tcaagccatt cttgtaaacg
      601 gatcgcatca ggaaagggac catgacatct gcgttgaaaa acgccatgcg cgccgtactg
      661 cgtggcagcg ggggatacgg ttgttttgtg gccggtacag gttttgagtc agtcagcgtc
      721 acttctgaga taacaaagcc ttcaccttcg ttcggggtgc gagcggccag gatgtttccg
      781 ctggcaccta cgactttaca acttggaatc atctcacagg acatttccag gcttttggcc
      841 tgagacaaaa aatttagaat acgaggcgcg aacagagcca ggcttgtgag aagggccctg
      901 gccctcggga ggtgcgtgcg aacttgtcca cttgcaccag aattcacggc aggtatacct
      961 aaccatttgg cctgctgatc caccatctgg ccgaagaatg ttttgcctac atctttgatc
     1021 gaactcattg tttccaaatc gttcatctct aatttttcat tatttggaaa ctcaaaggac
     1081 gcggcggacc cgtctggcgg acaactggaa attacgatca tatctgcttt gcctgcgtac
     1141 tgtttccaaa tatcaacatg accgacatcc cagcaaatca ttaagcccag gtctcccaat
     1201 tcggtcttgg ccacggtcat gccacgccgc ccgcggaaat aagcccgttc ccatgcccat
     1261 ggataattct tatcatatcg ccacctctgc ccactcgggc taaagagaag catggtgtca
     1321 tagatttctc ccccgtctaa cagcagaatt gctcccgcca ggtgaatgct tacacgctca
     1381 gacaaggttt tcatccaggt gctggtgagg ccttccagcg tctcagctcg ttcgaaattg
     1441 tcatcgctgt aggcgtatcc ggtattgaat agctctggca acacgaccag ttgtgcgccc
     1501 gcctgcgcag cttttgtgat gatcttttcg gcgcgtgcca ggcgttcggt aaggggcgct
     1561 ggcatcgcat ccatttgaac cgttgcgact ttgattgtcc gaggcatgtt gttttcctcc
     1621 aattacaaat acttcagcct gataaaaatt atagcatgat aagtttattg agaaattttc
     1681 atgccgcggg cttttgcgca ttacaattca tccatggctg attcgcttcg tgccctcaat
     1741 caggaaatca tcgcctgccg gaaatgtccg cgtctcgttg cctggcgcga ggaagtagcg
     1801 cgtgtcaaac ggaaggccta tcgcgatcag gaatattggg gcaagcccgt ccccggattt
     1861 ggcgactcca acgcacgtgt actggtcgtg ggtcttgcac ctggcgcgca tggctcgaat
     1921 cgcacagggc gtgcctttac tggcgatgca tcgggtgggt tcctgtatcc cgccctatat
     1981 cgcgccgggt ttgcaaatca gccgacggct gtgtcaagag cggatgggct ggtgctgaat
     2041 gatttatata tcgtagcgtc agcgcgttgt gtacctccgg acaataagcc aacccctgaa
     2101 gagttaagta attgccaacc ctatctcgaa cgtgatcttc aaattctcaa accaaaggta
     2161 attgtctgcc tgggacgaat cgcttttgaa aggatcctga aaatgttttc ggtgcgcaat
     2221 tcatcctgga agtttggcca tggcgctacc tatcgaatgg atgatggagt atggatcctg
     2281 tgttcttatc acccgagtca gcagaacaca ctgacgggta aattaacggt aacaatgttc
     2341 gatgaaattt gggcgaaggc aagggatttg cttcaggctg aaccagaggt aatagacaca
     2401 gccaacaagg catctgttta accatgcccc tcggacaaat cgcatttctt ggctctggtg
     2461 agacatcttt agcaggaggt cgcatttttg aagcccttgc gcggaaaata aacgaacctc
     2521 tccgcatcgc tttgatggaa acgcccgctg gtttcgagct gaattcgtca caggttgtag
     2581 gtaaagtggg tgattttatg gcgattcgct tgcaaaatta caaacctatt atcgatatcg
     2641 tcgcggctcg taaaaaggat tctccctaca gtccggacga ccctgagatc atcaaacctc
     2701 ttttatacgc aaatatgata ttcatgggac ccggcagccc gacctacgcg atccgccagt
     2761 tgaaagatac gcttgcctgg gatgtaatcc gtgcccggca tcgcctcggc gccacgctga
     2821 tatttgcctc cgccgcgacc atctccatcg gcgcccattc actacctgtg tatgaaattt
     2881 acaaagtggg gcaggatgtt catgctgtag acggtttgga tctattcaaa gatttcggcc
     2941 tgcatttatc tctcatccca cattggaata acgccgacgg cggggtggac ctggatacca
     3001 gtcgctgttt tgtgggtatg gatcgttttg cagagtggtg taatctcata ccctctgaaa
     3061 ataccactct gggactggat gaacacaccg gaattatgat cgattttacg aatggtattt
     3121 gtgaagtaag cggtgtgagt tcagtttcat tggttcgcaa atgtgaccct gagatctatt
     3181 cttccgggtc aaaatttgct ttgagcgagc tgggcgagat ctccattcct gatccggtag
     3241 aaaaagatat tcctgcggac gtctgggata tggttatcaa tgtaccctct cttcaagatg
     3301 atagtccatc ggatgaagtg gtcgcattag cggagcaacg cgtctctgcg cgagaaacca
     3361 aaaattggcc tgagtccgat aaattacgag accagatttc ggcgctgggc tggacggttc
     3421 aggattcaaa agatgggtat aagctagtcc ggaactgacc gtttcaagtt tgaatttagt
     3481 ggggagatta gattctgaaa tcacttctgc gataaaataa gattccaatc cttattaagg
     3541 agaatcccat gcgcgcagca gatgtcatca tcaaaaagag agacaaaggt gaactctctc
     3601 gggaagagat cgagttcttc ataaatggat ttgtttcggg ggaagtgcca gactatcagg
     3661 cttctgcatt tgcaatggcg gtcttgctta atggcatgac cgcgcgcgaa acgactgatc
     3721 tcacgctctc gatggctcat tcgggacaaa tgctggactt aagtgatgtc gttgacctgg
     3781 cagttgacaa acattcttct ggcggtgtgg gagataagac gagtattgct gttttgccaa
     3841 ttgtcgctgc atgtgggctt cccgtgggga agatgtctgg gcgcggactt ggtttcagtg
     3901 gcggcacgct cgataagctg gagtccattc ccggataccg cgtggattta tcgacggacg
     3961 agtttaagca gcagctcaga gaaaaaggaa ttgtcctcac gggtcagtcg ctggacctcg
     4021 cgcctgcgga cggtaaactc tatgccctgc gcgacgtgac aggaactgta caatccattc
     4081 cgctcattgc ttcctcaatt atgtgtaaaa aactcgcggc gggcgcgcag gcgattttgc
     4141 tggatgtcaa agtaggaaac ggtgccttca tggaaactct cgatgaagcg cgtgaacttg
     4201 caaacctcat ggtggatatc ggtaaactgg cgggtcgtga agtgattgct cttctttccg
     4261 atatgaacca gccccttgga aatgcagttg gaaactcgct ggaggtcatt gaagccattg
     4321 acatgctcaa agggcaggga ccggaagatt ttcgtgagca ttgtctgcat gtttgtgagc
     4381 atctcctggt gttgggaaaa cgcgccaagg acctggatga gggccgtgct cttgccgaaa
     4441 aatcaattgc agatggctcc gcaaccgaga aactccgtgt gctggtccgg gcgcaaggcg
     4501 gagatgtttc ttttgtagat gacacgtcga agtttccgcg tgcggagtat attgaagtag
     4561 tggagtcgcc gcaggacggc tacctgactc acatcctggc gcggactgtg ggggaaacgt
     4621 ccgtcacgct gggggccggg cgcgccaaga aatccgatcc tgtaaatcac gcagtgggct
     4681 tcctcatcca tcataaagtg ggggataagg ttgcgaaggg tgagccgctc tttacgatcc
     4741 atgccaatga caaggcaaag tctgcagagg tccgcgagct ggtccttgca gcacatcaat
     4801 tcagcgatac tcccgtggcc ccgctccctc tattttatga ctaatatgat gaattaaggc
     4861 tgtagacttg cataaatctt gggaatgggg catacttaat acaggagcaa aagttttatg
     4921 gcgaaggtac ctttgcgggc atacaaccgc gagatagaga caatgataga ccgcggtcag
     4981 ttggatgaag cgatcgcgca ttgtcatcat attttcaaaa cttttcccaa acatctcgaa
     5041 acctatcgtt tgctgggtaa ggcgtatctc gaatacaagc gatacaagga agcagtggat
     5101 gttttttcac gcgtgcttgc ctctgagcca aacgattttg tggccaacgt agggatgagc
     5161 attatccgcg atgaggaaaa taaactggat gatgccatct ggcacatgga acgcgccttt
     5221 gagacccagc cgtcgaatgc cgcgatacaa agcgagctcc agagattata tgggcggcgt
     5281 gatggtgttc aacccccgcg catccgcatg acgcgcggtg cgctggcgca tatgtatgta
     5341 cagggtgagt tgtatccaca ggccatctct gagatcaaaa gtgtcctaaa agaagaccca
     5401 ggccgcagcg atatggaaac attgttggcg cgtgcctgtt atcgcagcgg tttgaagaat
     5461 gaagctgcag atgcagcctc cagggttctc cgtagatatc catattgtct ggatgccaac
     5521 cgcgttttga tggagatttt cagtaccgat aaacctgaca atgcgcagat ctatcgtcaa
     5581 cgtgtttttg aactggatcc gtatgcagcg caggtgacgg gaacggtctt tcaatcaaat
     5641 gaagtcccgg atgcggctat cagccttgaa cacctggatt ggaatgggca gccggtgggc
     5701 actggagcac aagccgcctg gagtgagaac caggcaatta gcctggaaag tggcgtgagc
     5761 cgtgatgaac aaccggaatg gctgagaaac gcctacgccg ataccactcc accgacgaca
     5821 ccctctccaa cccgacccat cgatccaacg acaggcatgc cggtaccggc tttcgataca
     5881 tcagcgcagc ccggctcaaa ggatgatatc ccggactttt tacgcgcagc cggctgggga
     5941 caatcgactg gcgccttcga tgaaagcaag ccaatctttg gcacggatga acctgccgca
     6001 ccgccactcg aacagggtga gatcccggac tgggtcaaag ccatggcgcc ctcagaaacc
     6061 acggagtcta cacctgccgc ggacgaggaa ttgccagatt ggatcaataa gattggcacc
     6121 aattcattgc ccgttccgac accttcagac tcaggtcaag tggattggat gagtcaactg
     6181 ggtcaaactt ccgaagaggc gtcttcaccg cagactacag gcagtgacct ggattggatg
     6241 aatcaactcg gtcaagcgtc acagtcgatg actggtgaga gttcagtgaa cgatgaacca
     6301 gactggatga aatcgtcgga tcaacctgag tctgctctgt catcagctgc agccgatact
     6361 gcaccagact ggatgaatca acccgatcag ccacaacagg ctgcctctat ggagcaatcc
     6421 tcagatgatc agcttgactg gcttaaacaa ttagatcaac ctgaatcttc tgcatctgac
     6481 ccagcctctg aagaattgcc agagtggatg aagacctccg atcaacctat gcaatc
//
LOCUS       DASTJP010000524         4334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1324091, whole genome shotgun sequence.
ACCESSION   DASTJP010000524 DASTJP010000000
VERSION     DASTJP010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324091"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VFQ23_15385"
     CDS             <1..1128
                     /locus_tag="VFQ23_15385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET9908033.1"
                     /translation="FDFHGDPASALPEVLDPEQNSEFRDNYLEVAYDLSQVFFITTAN
                     TLETIPGPLRDRMEIIYLSGYTENEKVSIAKGYLIPRQIRENGLREEEVAFTAEALQK
                     IIREYTREAGVRNLERKIGAVCRKVGTRIAEGKLKKARITPQLVEEYLDHPIFQSTEE
                     LSQRMSIPGVVPGLAWTPYGGDVLFVEATSMPGGKGFQVTGSVGNVMHESARAALSFV
                     RSRAKQLSLDSEFFNRSDFHLHIPAGAQPKDGPSAGVTMATAIVSLVSGRKVKPQVGM
                     TGEITLRGQVLPIGGVKEKVLAAHRNGLRTIILPKRNEQDIDDVPDEIKKVMKFVFVE
                     SMDDVLDAALETNHKHPKSKAKTKKKAAQRKTKANGKNTAR"
     gene            1106..1450
                     /locus_tag="VFQ23_15390"
     CDS             1106..1450
                     /locus_tag="VFQ23_15390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9908034.1"
                     /translation="MAKILLAEDDPTMISLLKTLLKMEGFDVVMLDAYADVPAVVKQE
                     MPDTLLMDVHLGGQNGLQILESIRKNQDLAKVRVVMTSGLNMKEECISRGASAFLLKP
                     FMPDDLISVLKQ"
     gene            1470..1898
                     /locus_tag="VFQ23_15395"
     CDS             1470..1898
                     /locus_tag="VFQ23_15395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908035.1"
                     /translation="MPVQIRQFKFPSDYEQVLELWQNIEKGVRLGKSDSLTEIEKKIS
                     RDPDLFLVASTGGHIIGAVIGGFDGRRGLIYHLAVASSYRGHGIGSSLMEEVESRLRA
                     KGCLKCYLMVTLDNLTVSDYYTQRGWQPMEHVQLFGKELQ"
     gene            1895..2389
                     /locus_tag="VFQ23_15400"
     CDS             1895..2389
                     /locus_tag="VFQ23_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET9908036.1"
                     /translation="MSIRFGILTLSDRSSRGERPDSSGPALARLIEAHGWSVTRQSIL
                     PDDESTIREILIDWADGAELDVILTTGGTGFSPRDLTPEATRAVIERDAPGLAEAMRA
                     ASLQITPHAMLSRVVAGIRKKTLIINLPGSPKGAVENLQVVMPVLSHAIQLLREDPES
                     ETSH"
     gene            2462..3019
                     /gene="efp"
                     /locus_tag="VFQ23_15405"
     CDS             2462..3019
                     /gene="efp"
                     /locus_tag="VFQ23_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET9908037.1"
                     /translation="MIDVNELRKGVTFELDGGLYKVLDYSHNKTGRGNATIRVKARNL
                     LTGANIERTFNSGLSVQDVSLDFHNVSYLYTDGDFYHFMDNETFEQPAIKADMLGDDA
                     LYLKEGMEVKLTFYRGEPLDVEIPSSVDLKVVEAETAIRGDTATGVTKKVTTETGLLV
                     NCPQFVKVGDTIRVNTETGEYVTRV"
     gene            3127..3456
                     /locus_tag="VFQ23_15410"
     CDS             3127..3456
                     /locus_tag="VFQ23_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908038.1"
                     /translation="MKTPAGTECPHFYGDYFRGRSVEECRLLKAAGQEWTRDLCKSCP
                     VPEIARANACQFMQLRPKVGRPLSAAFLRRVQINAFCDKTHKKVSEPQIGCGECHALP
                     FKLEIKS"
     gene            3502..>4334
                     /locus_tag="VFQ23_15415"
     CDS             3502..>4334
                     /locus_tag="VFQ23_15415"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9908039.1"
                     /translation="MSLTLLLDLDDTLLYTNLDAFVPAYFQALAIHTAKYVPANALVN
                     ALGQGLSVMNESEDPTKTLQEVFEADFYPKLGMPKEELWDVFEDFYDNVFPTLAAITK
                     PRPEAASLIEWGLTCGFRIAIATDPFFPRTATYHRLRWAGLDPDQFELISTFEHFHFS
                     KTHPAYYAEILGRLGWPEGPVLMAGNDVRRDLLPAHQLGLKTYFVDGEATSSPDFVVG
                     RGNLSDLRLWLESVDFSSLEPSFKTPDSILGIMKSTPAVLQSVSSPLNDEEWRHEPTR
                     E"
BASE COUNT         1104 a         1015 c         1121 g         1094 t
ORIGIN      
        1 ttcgattttc atggtgaccc tgcctctgcc ttacccgaag tattggatcc ggaacaaaac
       61 agtgaattcc gcgataatta ccttgaagtt gcgtatgatc tctctcaagt cttcttcatt
      121 acaaccgcaa ataccctgga gacaatcccc gggcccttgc gcgaccggat ggagatcatc
      181 tatctttctg gttatacaga aaacgaaaag gtctcgatcg ccaaaggcta cctaattccg
      241 cgtcagattc gcgaaaatgg ccttcgggag gaggaagtcg cctttacagc tgaagcactg
      301 cagaaaatca ttcgcgaata cacccgcgaa gcgggagtaa ggaatctgga gcgaaaaatt
      361 ggagcagtct gtcgaaaagt agggacgcgg attgctgaag gcaaattgaa aaaagccaga
      421 atcacgcctc agctggttga ggaatatctg gaccatccta tttttcaaag taccgaagag
      481 ctcagccagc gtatgtcgat tcctggtgtg gttccagggc ttgcctggac cccctacggc
      541 ggtgatgttt tatttgttga agcgacaagc atgcccggag gtaaaggctt tcaggttact
      601 ggttctgtgg gaaatgtgat gcatgaatct gcgcgcgcgg cgctgtcgtt tgtgcgctcg
      661 cgtgccaaac agctcagctt ggattccgag ttctttaatc gatctgattt tcatttgcac
      721 attccggcgg gcgcccagcc gaaggatgga ccatcggctg gggtaacgat ggcaacggcc
      781 attgtttcac tcgtgtcggg ccggaaggta aagccgcagg ttggcatgac cggtgaaatc
      841 acgctcagag ggcaggtgtt gcccattggt ggcgtaaagg aaaaggtttt ggctgcgcat
      901 cgcaacggct tacggacaat tatcctaccc aagcgaaacg aacaggatat tgacgatgtg
      961 ccagatgaga tcaagaaggt gatgaagttt gtttttgtgg aatcaatgga tgacgtatta
     1021 gatgcggcac tggaaacgaa tcataagcat cccaagtcaa aagcgaagac aaagaaaaaa
     1081 gccgcacaaa ggaaaacaaa ggcaaatggc aaaaatactg ctcgctgaag atgacccgac
     1141 tatgatttcc cttttgaaga cgttgctcaa aatggaaggc tttgatgtag taatgttgga
     1201 tgcctatgca gatgttcctg ccgtcgtcaa acaggaaatg ccggatacct tgttgatgga
     1261 tgttcatctt ggcgggcaga acggtttaca aatccttgag tcgattcgga agaatcaaga
     1321 tcttgcaaag gtccgcgttg tgatgacctc cggtctcaat atgaaagaag aatgtatcag
     1381 ccgcggtgca agcgcattcc ttctcaagcc attcatgcca gacgacctga ttagcgtatt
     1441 gaaacaataa tacagccaca gtgacgccct tgcccgttca aattcgacag ttcaaatttc
     1501 cttcagatta tgagcaggta cttgaattgt ggcagaatat tgaaaaagga gtgcgccttg
     1561 gaaaatccga ttcgttgacg gaaatcgaaa agaaaatcag tcgtgatccc gatctttttc
     1621 tggttgccag tacgggcggg catatcatcg gagcggtaat tggtgggttt gacggccgac
     1681 gtggattgat ctatcacctt gcggtggctt ccagctatcg tgggcatgga atcggttcta
     1741 gtctcatgga ggaagttgag tcgaggttgc gcgcgaaggg ttgcttgaaa tgctatttga
     1801 tggtgaccct ggataattta acggtttcag attactacac acagcgcggc tggcagccga
     1861 tggagcacgt ccagctattc ggcaaggaat tgcaatgagc atccggtttg gcattcttac
     1921 cctttcagac cgatcctccc gcggcgaacg cccagattct tccggccctg ctctggcacg
     1981 ccttatcgaa gcgcacggct ggtctgtaac ccggcaatct attctccctg atgatgagtc
     2041 taccatccgc gaaattttga tcgactgggc agatggggcc gaattggacg taattcttac
     2101 gactggtggc acaggctttt ctccgcgtga cctcactccg gaggccacac gagcagtcat
     2161 tgaacgcgat gctcccgggc ttgcagaggc gatgcgagcg gccagcctac agatcacgcc
     2221 gcatgcgatg ttatcaagag tggtagccgg tatccgcaag aagaccttga tcatcaattt
     2281 acccggcagt ccgaaaggcg ccgtggaaaa tctgcaagta gttatgccag ttctttcgca
     2341 cgccatccag cttttgcggg aggatcctga atcggagacg tcacactgat cttgaggcgc
     2401 tactgcctgg ctctctggta taatcccgcc gcttgaaatc tcactaaagg aaaatgcagc
     2461 aatgatcgac gtaaatgaac ttcgcaaagg tgtcactttt gaattggatg gtggtcttta
     2521 taaagtatta gactatagcc ataataaaac aggtcgtggg aatgccacga ttcgggtgaa
     2581 ggcgcgtaat cttctcactg gcgcaaatat cgaacgcact tttaattcag ggctgtccgt
     2641 tcaggatgta agcctcgatt tccataatgt gtcctacctt tataccgacg gtgattttta
     2701 ccattttatg gataatgaga cgtttgagca gcccgctatt aaagcggata tgcttggcga
     2761 cgatgcctta tatctcaaag agggcatgga agtaaagctg actttttacc ggggcgaacc
     2821 tctggatgtc gaaattccat cctctgtcga tctgaaggtc gtagaagcag aaacagccat
     2881 ccgtggggat acggctaccg gcgtgaccaa gaaggtcaca acggaaacgg gtctactggt
     2941 taactgccca caatttgtaa aagtggggga tacgatccga gtgaacacag aaacgggtga
     3001 atatgtaact cgtgtgtaac cgtcggaacg aaattcaaac ggttttccag atgagtgagt
     3061 ggctatcgta ataaaccagg cctctcgctc atcacttatt tatattatca ctctataatt
     3121 caggttatga aaactccagc tgggactgaa tgtcctcatt tttatggtga ttatttccgc
     3181 ggtcgctcgg tggaagaatg ccgtctgcta aaagctgccg gtcaggaatg gacacgagat
     3241 ttgtgcaagt cttgtcccgt acctgaaatt gccagggcaa acgcctgtca gttcatgcag
     3301 cttcgcccca aggtggggcg tcctttgtct gcagcatttc ttcgtcgagt ccagatcaac
     3361 gccttttgcg ataaaaccca taagaaggta tctgagccac agatcggatg tggggaatgt
     3421 catgctcttc catttaagct cgaaattaaa tcctgaattt tcataaaggg gaaattttat
     3481 cgttggaaag ctaattgact catgtctctc acattattac tggatcttga cgacaccttg
     3541 ctttatacca acctggatgc atttgtgcct gcatactttc aagcgctagc aattcacaca
     3601 gcaaagtatg taccggcaaa tgcacttgtc aacgcgcttg ggcaaggttt gtcggtgatg
     3661 aacgaaagtg aggacccaac gaaaactttg caggaagtct ttgaagctga tttctatccg
     3721 aaacttggca tgccgaaaga ggagttatgg gacgtattcg aagactttta tgacaatgtt
     3781 tttcccaccc ttgctgcgat tacgaaaccc aggcccgaag cagcttcgct gattgaatgg
     3841 ggcttaacct gcgggtttcg aatcgcgatc gctactgatc ccttttttcc acgcacagcc
     3901 acgtatcatc ggcttcgctg ggcgggcctc gaccccgacc aatttgagtt gatttcaaca
     3961 ttcgaacatt ttcactttag caaaactcat cccgcttatt acgcagaaat actcggacgg
     4021 cttggttggc cagaagggcc agtccttatg gcagggaacg atgtgaggcg agatttgttg
     4081 cccgctcacc agcttggatt aaagacttat ttcgtagacg gagaagccac ctccagcccg
     4141 gactttgtag tggggcgggg caatttgtct gaccttcggc tctggttgga atccgttgac
     4201 ttttcttcgc tcgaaccatc cttcaagacc cctgactcga tcctggggat catgaagtct
     4261 acgccggctg tgttgcagag tgtatcctcg ccgctcaatg acgaagagtg gcggcacgag
     4321 ccaactcgcg aaga
//
LOCUS       DASTJP010000525         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1326329, whole genome shotgun sequence.
ACCESSION   DASTJP010000525 DASTJP010000000
VERSION     DASTJP010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326329"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VFQ23_15420"
     CDS             <1..1607
                     /locus_tag="VFQ23_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908040.1"
                     /translation="GATSLRALLYMDEMFGYMPPQRNPPSKQPLLRMLKQARAFGLGL
                     LLATQNPVDVDYKALSNAGTWFVGKLQTEQDKNRLLDGLESAAGGIPRAEFDKLISSL
                     GKRVFVLHNIHAKEPELFQTRWAMNFLAGPLTRNQIPDLNKLAGAVVDTSTVIRGSAP
                     ASLSAFQPVAVVTPHSTPGEAPPQPPMATRPSSMVSRQSEIDGSQTKPPIPAWIKEYF
                     LPQNYSLPEAFSAANQAMPHEVTIEGVVYRPALLASAQVRILDRKLGVDSEITRSILV
                     KSVDKRGVVRWEENPYSGPALDQIDTSPVPGARFSTIDSPLNNTKLMTSLQKDFSDWV
                     FRNSSVRARANQALKVYAGPDVSQADFVRACADAARDARDAEIAKKTAPLERQLKTLE
                     DKLTREERELREDEAELSNRKLEEAGTHLENITGLFGGRRKASRLSSSLTKRRMTEQA
                     KADVEESITAIDQFKQQIAELQKRREEVVAEINNRWGNVVNESTEVTVTPKKTDVFVN
                     LFGVAWTPYYIVQMDGETVEISAFGA"
     gene            1989..>2333
                     /locus_tag="VFQ23_15425"
     CDS             1989..>2333
                     /locus_tag="VFQ23_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908041.1"
                     /translation="MATVSYPYQKTPVVPQIVAALISGVVLFFGLIIAWVLGYQLIYA
                     GRIFPGVSVAGVDLSGMTPSDAALKLNQSLSYPINGKILFRHGEQVWVATPAQLGMVF
                     DPSSSAVTAYELG"
BASE COUNT          564 a          605 c          596 g          568 t
ORIGIN      
        1 gcggcgcgac atcattgcgc gccttgcttt acatggatga gatgtttggt tatatgccgc
       61 cacagcgaaa cccgccttcc aagcagccgc ttctgcgcat gttgaagcag gcgcgagcgt
      121 ttggtctggg cttattgctt gccacgcaga atcctgtcga tgtggattac aaagctctct
      181 ccaacgctgg cacatggttt gttggcaagc ttcaaaccga gcaggataag aaccgattgc
      241 tcgatggtct cgaaagcgcg gcaggcggca tcccacgcgc cgagttcgac aaactaatct
      301 cgtcgctggg caaacgtgtt ttcgtccttc ataacatcca tgccaaagaa cctgagctct
      361 tccaaacgcg ctgggcgatg aactttcttg ccggaccatt aacacgcaat caaattccgg
      421 acttgaacaa actggctgga gcggttgtag atacgtcaac agtgataaga ggatcagccc
      481 ctgcctctct ctctgccttt cagcctgttg ccgttgtaac tccccattcg acgcccggag
      541 aggcaccacc tcaacctccg atggcaacgc gaccatcgtc catggtcagt cgtcaatctg
      601 aaatcgacgg tagccagacc aagcctccca tcccggcctg gatcaaggaa tatttcctcc
      661 cacagaacta cagcctgccc gaggccttct cggcggccaa tcaagctatg ccacatgaag
      721 tgacgatcga aggtgtggtg taccgtcccg cactgcttgc ctccgcgcag gtgcgcatcc
      781 tcgaccgcaa actcggcgtg gatagtgaaa ttacgcgatc gatcttagtg aagtcggtag
      841 ataagcgcgg tgtcgtgcgt tgggaagaga atccatatag cgggcctgct cttgatcaaa
      901 ttgatacgtc cccggtgcct ggcgcaaggt tcagcacgat cgactcaccg ctgaataata
      961 caaagttgat gacctccctg caaaaagatt tttcggattg ggtatttcgc aattcgtctg
     1021 tgagggcacg cgccaaccaa gcgctgaaag tgtatgcggg cccggacgtc agccaggcag
     1081 atttcgtacg agcctgtgcg gatgccgccc gcgacgcgcg tgatgctgag atcgccaaga
     1141 agacagctcc gctggaacgt cagctcaaaa ccctggaaga taaactgacg cgggaggaac
     1201 gcgaactgcg tgaagatgag gctgagcttt caaatcgcaa actggaagaa gccggaactc
     1261 acctcgaaaa tatcaccgga ctcttcggcg gacggcgcaa ggcctcgcga ttatcatcgt
     1321 ctctgaccaa gcgccgtatg accgaacagg caaaagccga cgttgaggaa tctatcacgg
     1381 caatcgatca gtttaagcag caaattgcag agcttcaaaa acgtcgcgag gaagtcgtcg
     1441 ctgagatcaa taacagatgg ggaaatgtcg taaacgaaag cacggaggtc actgtcacac
     1501 caaagaaaac ggatgtgttt gtgaatctgt tcggcgtggc atggactcct tattacattg
     1561 ttcaaatgga tggcgagacc gtagagatat cggcgtttgg tgcgtgaata attttatagg
     1621 tcattgcgag gcgggcgaag cccaacgaag caatccccgt taaggacatt cacttctata
     1681 caaagttttc atcgtataag cggagattgc ttcgtcgcac ccgtgcctga tttgctcgag
     1741 caaatcacgg gcggtgctag ggacgaacgc tcctcgcaat gacagtacaa ctttttgtgg
     1801 aacaaatgat tcttgtattc cgtcttaatc gagtgcttaa tcaagcagca ttactcattc
     1861 cccattgccg ccgtgtcatt aataagtaaa ttttgaatgg atttgagtgc caaattcaaa
     1921 tcttaatctt tattaatttt gcggcacttt gtaagctttc cattttcatc agggtataat
     1981 tttcaactat ggctactgta tcctatcctt accagaagac acctgttgtc ccccaaattg
     2041 ttgccgcttt gatcagcggt gtcgtgttgt ttttcgggtt gattattgct tgggtattgg
     2101 gctatcaact gatttatgcc gggcgtattt tccccggtgt atctgttgcc ggtgtggatc
     2161 tctccggaat gactccatcg gacgcagctc tcaagttgaa ccagtcacta tcctacccga
     2221 tcaatgggaa aatcctcttt cgacacggtg aacaggtgtg ggtggcgact cctgcgcaac
     2281 tgggtatggt gtttgatcca tcttccagtg cggtaaccgc ctatgaactt ggc
//
LOCUS       DASTJP010000526         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_499897, whole genome shotgun sequence.
ACCESSION   DASTJP010000526 DASTJP010000000
VERSION     DASTJP010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499897"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /gene="era"
                     /locus_tag="VFQ23_15430"
     CDS             complement(<1..756)
                     /gene="era"
                     /locus_tag="VFQ23_15430"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET9908042.1"
                     /translation="MNEFRSGFIAIVGRPNVGKSTLVNALLGQKIAAVSPKPQTTRKR
                     QLGILTTDTAQLVFVDTPGMHTARHKLGQFLNQEAEESLQDIDVILWVVDISSRPTDE
                     DKEIASLLARTPRRTPLVLVANKTDLIPAEVLTARLEAYQASLKKDAKVISISATQNK
                     NLDQLQELLVSLSPVRPPEFDEEQVTDSYERDIAADLIREACLHHLRDEVPHGVGTRI
                     DEFAERENGMLYIAATIFVERNSQKGILIGEGGK"
     gene            complement(753..2390)
                     /locus_tag="VFQ23_15435"
     CDS             complement(753..2390)
                     /locus_tag="VFQ23_15435"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET9908043.1"
                     /translation="MMKADAVENAPIPERYEFIIPLLLFLVFLALTLPGISWGAPSIW
                     HPDEVVYIAIRGLYEGVEFDAANFNHPHLPIYAMYWLGKILIALGQTDTEVLVGARVL
                     SAVIAGLTIVLSYLLPRRMGYNIYVSALSGLLLLAVSEMSHNGRFAHNDTFVTFFCTL
                     TIFFIVQYINKNHRGWLYAAFFAAGIAVSSKYIAITLVLIPMIVYLWTRRNAGLKLSL
                     ETFETLFISGALTYLGYAIGTPKALTWMVYYFKRLIPALIYNSNYGVQPDSLRGILGQ
                     YSVFLDAVGLPLFLLFVAGLLWGVYKVVQAYQAKAFDRETYRGLLLLTILVLDLPILV
                     SYNYPTRFFLPLMPFFAILSALFIRDLYGLAQQNVNPNYTRVIGTGLTLLILFSLGRS
                     VSVMLLFLNDARIPATSYLNTLTPGTSLEHTEYPPSIPGSFFEREHNYPIHFRNTPDE
                     PLLTHKNYVYNIGEEGLNERQTTYLVIDSFTSDKFDSPYTCELMQVECDFFKQLDAGE
                     SAHYKLIADFSYQLPPFLPQLDVSFVNPEIRIYERVP"
     gene            complement(2400..3296)
                     /locus_tag="VFQ23_15440"
     CDS             complement(2400..3296)
                     /locus_tag="VFQ23_15440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9908044.1"
                     /translation="MKTKHWIVFILLGAIWSSSFLWIKIGVQDIGPMALTAFRMLFGA
                     ITAVMIGLYQKVKWPRDWKTLGIFAILGPTSLAIPIFFISWGEQSIDSAVASILNATT
                     PLFTILIAHFLLQDDKMTFQKTLGLLVGFGGVVVLMSKDLTAGAQNSILGQGAVILAS
                     LFYAGSAVFGRKLTQHVEGTVRGAMFLITSTIFMWIVGPLAERPFEFPTESITWIAIL
                     FLGILGSGLAVIMLWYLIHEVGPTRASLVTYLFPVGGVILGVIFLNEQLSWQLLLGSI
                     LIVASLAVVNWQPVRRAIPKEA"
BASE COUNT          971 a          830 c          884 g          861 t
ORIGIN      
        1 cttgcctcct tcaccgatca aaatcccctt ttgtgaattg cgctccacga aaattgtcgc
       61 cgcaatgtag agcattccat tctcgcgctc ggcgaattca tcgatccgcg tgccgactcc
      121 gtggggtact tcgtcgcgta aatgatggag acaggcttca cggatcaaat ctgctgcgat
      181 atcgcgctca tacgaatctg tgacttgttc ctcatcaaat tcaggcggtc gcacaggact
      241 cagcgatact agtaactcct gcaattgatc gagattttta ttttgtgtag cagagatgga
      301 tatgaccttc gcatccttct taagagatgc ctgatacgct tcgagacgcg cggtcaacac
      361 ttccgcgggg attaggtccg ttttgttggc taccagcaca agcggagtcc gacgtggagt
      421 gcgagccagc agcgaagcga tctctttgtc ctcatccgtt ggcctggaag agatatccac
      481 cacccacaga atcacatcaa tgtcttgcag tgattcttcc gcttcctgat tcagaaattg
      541 acctaattta tggcgcgcag tatgcatccc cggcgtatct acaaaaacaa gctgcgcagt
      601 atccgtagtg agaatcccca actgacgctt gcgcgtggtt tgcggcttcg gtgaaactgc
      661 cgcaatcttt tgacccagca atgcattgac caacgtagac ttgccgacgt ttggacgtcc
      721 gacgattgca ataaagccag agcgaaactc attcatggga ctctctcgta aatacggatt
      781 tctggattca caaaggatac gtccagttgc ggcaggaacg ggggaagttg ataagaaaaa
      841 tctgcgatga gcttgtagtg tgcggattct ccggcatcca gttgtttgaa gaaatcacat
      901 tccacctgca tgagttcaca agtgtatggg ctatcgaatt tatctgacgt gaaactgtcg
      961 atcacgaggt aggtcgtctg gcgttcgttc aacccttctt cgccgatgtt atagacgtaa
     1021 ttcttgtggg tgaggagggg ttcatcgggc gtgttccgaa aatggatggg gtaattatgt
     1081 tcgcgctcga aaaaacttcc cgggattgat ggaggatatt ccgtatgttc caaggatgtg
     1141 ccaggagtta aagtgttgag atagcttgtg gccggaatcc gcgcgtcatt gaggaaaagc
     1201 agcatcacac taaccgaacg ccccagcgag aagagaataa gtagggtaag acctgtgccg
     1261 attactcttg tatagtttgg atttacattt tgttgtgcga gtccatatag gtcccttatg
     1321 aaaagcgcac ttaagattgc gaaaaaaggc atcagaggca gaaagaaacg agtcggataa
     1381 ttgtaggaaa ctaggatcgg aagatcgagc acaaggatgg tcagtaaaag aagacctcta
     1441 tatgtttccc gatcgaacgc ctttgcctga tatgcctgta cgactttata gactccccaa
     1501 agaagacccg cgacaaagag aagaaataat ggaaggccta ctgcgtccaa aaaaacagaa
     1561 tactgtccga gaatgcctct gaggctatca ggttgaacgc catagtttga gttgtagatc
     1621 aacgctggaa tgagacgctt gaagtaatag accatccatg tcagggcttt gggtgtgcca
     1681 atcgcgtaac cgagataggt gagtgcgccg ctgatgaaaa gagtctcgaa tgtttcaagg
     1741 gagagtttca agcccgcatt tcgccgagtc cacaagtaaa caatcattgg aatcaataca
     1801 agggtaatgg cgatgtattt actggatacc gcgattcccg ctgcaaaaaa tgcggcgtat
     1861 aaccagcctc tatgattttt gttgatgtat tggacaataa agaaaatagt cagtgtgcag
     1921 aagaaggtga cgaatgtatc gttatgagca aaacgaccgt tgtgagacat ttcgctgacg
     1981 gccagcaaga ggagtccact taatgctgag acatagatgt tatatcccat tcgacgggga
     2041 agcagataag acagcacgat tgtcagtcct gctatcaccg ccgaaagcac gcgcgccccg
     2101 actaatacct ctgtatccgt ctgcccgagg gcgatcagga ttttccccag ccagtacatg
     2161 gcataaatag ggaggtgtgg gtgattgaaa ttcgcggcat cgaattccac gccctcataa
     2221 aggcccctta ttgcaatata gacgacttcg tccggatgcc agatgcttgg cgcaccccat
     2281 gagatacccg gcagtgtcaa tgccaggaat accagaaaca ataatagagg gatgatgaat
     2341 tcatatcgct ccggaattgg agcgttttcg accgcatcag ctttcatcat cccataattt
     2401 tacgcctctt ttggaattgc tctgcgaacc ggctgccagt tgaccactgc caggctggca
     2461 acgatcaaga ttgatccaag gaggagttgc cacgaaagct gctcgttcaa gaagattacc
     2521 ccaaggatta caccaccaac tgggaagaga taagttacta acgacgcgcg tgtgggtccc
     2581 acctcatgga tcagatacca gagcatgatc acggctagtc ccgagcctaa gatgcccagg
     2641 aaaagaatgg cgatccaggt aatggattca gttggaaact caaatggtct ctcagccagc
     2701 ggccccacaa tccacataaa gattgtggat gtgataagga acatcgcacc gcgcaccgtg
     2761 ccttccacat gctgggtcag cttccgcccg aagactgcgc tgcctgcgta aaagagggaa
     2821 gcaagtatca cagctccctg tccgaggatc gaattttggg cacccgcggt cagatctttg
     2881 ctcatcagca caactacccc gccaaatcca accaacaagc ccagggtttt ctgaaaggtc
     2941 attttgtcat cctgcaacag gaagtgggcg atgaggatgg taaataatgg ggtggttgca
     3001 ttaagaatcg atgcaacagc cgagtcaatg gattgctcac cccaggagat aaagaagatg
     3061 ggaattgcca ggctggtggg accaaggatg gcaaaaatac ccagagtttt ccaatctcgt
     3121 ggccatttca ctttttgata gaggccaatc atcacagccg tgatggctcc aaataacatg
     3181 cgaaaagcag tcaatgccat gggaccaata tcctgcacgc cgatcttgat ccacaggaaa
     3241 gacgagctcc agatggcgcc aagcagaatg aatacgatcc aatgtttggt tttcaatacg
     3301 actcctttta gtgggtcgga ttggcaatcc gacttacgaa ctaacaatcg tttcgatgac
     3361 gtgcagggga caaatattac gctgctccag tagggttttc gttcctacct gaagaggtat
     3421 catctctata ggactgttat tgtatgaaaa tcgcgtcttg ctaaccaccc gtttcttgct
     3481 ctgtttctga aaaggagagt tattctattt tgacgtataa tattaaaatt catcagttac
     3541 atagtt
//
LOCUS       DASTJP010000527         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_341180, whole genome shotgun sequence.
ACCESSION   DASTJP010000527 DASTJP010000000
VERSION     DASTJP010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341180"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFQ23_15445"
     CDS             <1..197
                     /locus_tag="VFQ23_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908045.1"
                     /translation="GASIKQIFSDAYSWMPIMSHVKQFRYVRDSRNILENEMKEETLK
                     VNITKGNRERNRLKESTEAK"
     gene            250..522
                     /locus_tag="VFQ23_15450"
     CDS             250..522
                     /locus_tag="VFQ23_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HET9908046.1"
                     /translation="MSTKMHFTIEQARELGDKLGIDWSRFDVEQFRMGLDVELEHGLP
                     DPSTDVTGNDPILTGKIALAHLNEFPDYYTRLAKMEQEAEEERNQS"
     gene            838..1812
                     /locus_tag="VFQ23_15455"
     CDS             838..1812
                     /locus_tag="VFQ23_15455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9908047.1"
                     /translation="MNKTFVDWKRVWIFITFAFGIAWLVALALYFTGGLKPNTSTILV
                     LAVGYMGAPALAHILTRLVTREGWQGLYLRPNFRQGRLYWLICWVAPAVFTFTGMAIF
                     FGLYPQYFDRSLTAVKQLMESSALAEGQPTPAINPWVIVISQTLTALLIAPILNAIPI
                     LGEEFGWRAYLQPKLMPLGGRKTMLLMGIIWGLWHAPLIAMGHNYGMEYPGAPWLGIL
                     TTLWIMFILGTFLGWAVLRAGSVWPAVIGHGAFNGIAGIYVFFMQGNPNLLLGPSAAG
                     IIGSLGLAVFALIIFLKRDALKVVDVKIPIPQKDAIQIVTPSMKQTGD"
     gene            1815..1964
                     /locus_tag="VFQ23_15460"
     CDS             1815..1964
                     /locus_tag="VFQ23_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908048.1"
                     /translation="MNSRTEGAFAIIAALIVLLSAMWDPRVSVALSIIALVGLGIFKF
                     MRRSS"
     gene            1999..>2263
                     /locus_tag="VFQ23_15465"
     CDS             1999..>2263
                     /locus_tag="VFQ23_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908049.1"
                     /translation="MLDSIPVYTSVEGKAEILQAYQAIMDKWPVSYKELTIPTSFGDT
                     HVIASGPRDAPPVVLLHALFATAASWYRNVEAMSRFYRVYAVDV"
BASE COUNT          577 a          527 c          582 g          577 t
ORIGIN      
        1 taggtgcttc aatcaaacag atttttagcg atgcctatag ttggatgccg atcatgtctc
       61 atgtaaaaca atttcggtac gtgcgtgact ctcgcaacat ccttgagaat gagatgaaag
      121 aagaaacact caaagtaaac atcacgaaag gcaacaggga gcgtaaccga cttaaggaat
      181 caactgaggc gaaatagact atccacttgg gagctacagt gcctcaaaaa aattcataag
      241 gagagaaaaa tgtccacgaa aatgcatttc accattgagc aagcccgcga actcggtgac
      301 aaattgggta tcgattggag ccgcttcgac gtggagcagt tccgcatggg tttggatgtt
      361 gaattggagc atggtctacc cgacccctca acggacgtga caggcaacga tcccatcctc
      421 actggcaaaa ttgcgctggc gcacctcaat gaattccctg attactatac ccgtcttgca
      481 aagatggagc aggaagccga agaggagcgc aatcagagct aaaccatcca atcaaaccaa
      541 aggataaacc tcctataaga gtcaatacaa gaaacaagga gtattaagaa ttgcatattg
      601 cttttagcgc cgcggccgag acatgcgtaa actatccatg tgtctagtac aaaaactccc
      661 ccagtgatag gggagtcttt tgtttccgac cactatgatg cagccagaga aaatcgaatt
      721 caaagagttc gcgatgattt taaggatcat ccctcattct gtgtagggat ttgtcaagct
      781 gttccggtta ttaagcggga agactgccaa gataggagat acaagaggag tcggagaatg
      841 aacaagactt tcgtcgattg gaaacgcgtt tggattttta tcacctttgc ttttggcatc
      901 gcctggcttg tcgcgctggc gctctatttt acgggtggtc tgaagccgaa caccagcacg
      961 atattagtgc tggcagtggg ctacatgggt gcgccggcgt tagctcatat ccttacgcgc
     1021 ctcgtcacgc gcgagggctg gcagggactt tacctgcgcc ctaatttcag gcagggcagg
     1081 ctgtactggt tgatttgctg ggttgcaccc gctgtcttca catttactgg catggcaatt
     1141 ttcttcggtc tgtatccgca gtatttcgat cgatctttga ctgctgtgaa gcagttgatg
     1201 gaaagctcgg ccttagcaga gggtcaaccc acgcctgcca ttaatccgtg ggtcattgtc
     1261 atatcgcaga cgcttaccgc cctccttatc gcacccatcc tgaatgccat tcccattctg
     1321 ggggaggaat tcggctggcg tgcctatcta caacccaaac tgatgcctct cggcggaaga
     1381 aagacaatgc tgttgatggg catcatttgg ggcctctggc acgcaccgct cattgctatg
     1441 ggtcacaact acggcatgga atatcccggt gcaccctggc tgggaattct gacaacgtta
     1501 tggatcatgt tcatccttgg tacgttcctt ggttgggcgg tattgcgtgc aggaagtgta
     1561 tggcccgcgg tcatcgggca cggagccttc aatgggatcg ccggtatcta tgtgtttttc
     1621 atgcaaggta atccaaactt gttactgggt ccatcggcgg ccgggatcat cggttcgctg
     1681 ggtctcgcgg tgtttgcatt gattattttc ctgaaacgcg atgctctgaa ggttgtagat
     1741 gtaaaaatcc cgattcccca aaaggacgct atacaaatcg tcacacccag catgaaacaa
     1801 acaggagatt gaaaatgaac tcaagaaccg aaggtgcatt cgccatcatc gccgcattga
     1861 tagtattgct cagtgccatg tgggatccgc gagtttcagt ggcgttgtcc atcatcgcat
     1921 tagttggatt gggaattttc aaattcatgc gcagaagtag ttaagccgca gctcacaatt
     1981 atcttgagga gtctacccat gttggattcg attcccgtat acacttcagt tgagggtaaa
     2041 gcagagatcc tgcaagcgta ccaggctatt atggataaat ggcccgtttc ttataaagaa
     2101 ttgaccattc ctacaagctt tggcgacacg catgtaatcg cgagtggacc cagggatgct
     2161 ccgccggtgg tgttgttaca tgcgctgttt gcaactgccg cgtcttggta tcgaaacgtg
     2221 gaggcaatga gtcggtttta cagggtttat gcggtagatg taa
//
LOCUS       DASTJP010000528         4654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_182582, whole genome shotgun sequence.
ACCESSION   DASTJP010000528 DASTJP010000000
VERSION     DASTJP010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182582"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2157
                     /locus_tag="VFQ23_15470"
     CDS             <1..2157
                     /locus_tag="VFQ23_15470"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9908050.1"
                     /translation="SLVGGRIAINLKNTLLFYGILEIGIALFGVVSFSIIQWIGQSLA
                     GSPYVLVFLISFLVLLIPTFLMGMTLPLLTQSFVDRVETSGRVIGVLYGINTLGAAIG
                     CALAGYIFIGFLGLDGTIYIAVLLNAIVGLCAFFLSPWQRTEIIKSESVPTTDISNNV
                     LGYRTILLSSFLVGFLGLGFEILWIRVLMIVNKNTAYSFPSILFIFLLGLAIGGAIWG
                     RKADTSSNPAQLFCKIEMAGAAVAVFTFLGFWISVQGDASWLGNFFETQKPSLPFLRI
                     DREWSFSKMALVANLWSYFLPILILVLPATLVLGGGLTILDRLSINNPLVSGQRVGDI
                     HLANIIGSVAGTFVTSFILLPGIGSEWTLKLFALSTFLFPAFYFLDTRSKPTVQNLSS
                     LMVIGSVLLIGIILLPSRGEFYTRLYAAGSEQEVVVSESGDSVLALTYESRGINEKGL
                     LWIGGEINSFFPPEGLYESRAMVCAGASTPKRILVIGLGGGYITMFFKSIPTVEEIVI
                     VELLEDLSPFLTENQASIRETLADPRITYIVDDGRRYLNAFPDEKFDLISIDPLREHT
                     AGHNNLYSKQALEIYRNHLTENGVLCAWMAERHMIPHSVAQVFPYTDQFANEYMIASN
                     NPIQYDRAYMENASADFDDLAKSIYGSDFAAIPDVEHNLSFFLRDQNQILIEESGTPV
                     LDDSHPLLEYFLFRKPARREITPLPDGQLEFQRRLN"
     gene            2164..2577
                     /locus_tag="VFQ23_15475"
     CDS             2164..2577
                     /locus_tag="VFQ23_15475"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9908051.1"
                     /translation="MNKRFIILAICCVLISGCAMGVEAVTPIAVSATATPVPAGDLGF
                     GKIHGKVTDDVTGAPIAGAVITCEHHSFTSPATRSGTAMTDANGMYKFENVYFHDTDT
                     IKLTVQATGYQTQEISSAFFRMAELEANVTLNRDP"
     gene            2602..3012
                     /gene="tnpA"
                     /locus_tag="VFQ23_15480"
     CDS             2602..3012
                     /gene="tnpA"
                     /locus_tag="VFQ23_15480"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HET9908052.1"
                     /translation="MPYWKLYYHFIWGTKNRFPLIDSVLEPELYRAIAAKAQKLGGFV
                     HAIGGIEDHVHLSVSIPPKLAPAKFIGDVKGNSSHFVNYVIKPDFEFRWQAEYGVVSF
                     GERNLASVGRYIHNQKQHHAEGTLIAAMERMDEL"
     gene            3303..>4654
                     /locus_tag="VFQ23_15485"
     CDS             3303..>4654
                     /locus_tag="VFQ23_15485"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HET9908053.1"
                     /translation="MMNFIPRPSQEQILRYNGGRLGIAAVPGAGKTQILSALAAQIIS
                     SGALGDDQEVLIVTLVNSAVDNFEARIKRFFDNPLHALYKYRVRTLHGLAHDIVREKP
                     ARVGLEERFAILDEREADFIRREAVNAFLSTNPGYLDDYLDPALDESKRDWVRRQQLP
                     DMMESLVLAFIRSAKDRRLTPDRIRQLLDQAPASLPLAEMGLAIYSDYQRALAYRGAV
                     DFDDLIRLSLDLLENDEEYLERLRHRYPFILEDEAQDSSVTQERILSLLSGEGGNWVR
                     VGDPNQAIFETFTTASPELLREFIRNNNHVDMNESGRSQQFIIDLANHLIDWVMTEHP
                     ASQARNALDTPYIEPVPEDDPQRNPDEDKSAIRFAEKRLTPEEEVDAVVKSIQRWLPE
                     NTDRTVAVLVPRNARGVEVIDALKKKGIEVIEFLASTSNTRAAAGALNYLLSYLADPQ
                     S"
BASE COUNT         1109 a         1122 c         1192 g         1231 t
ORIGIN      
        1 tcactggtcg gcggacgaat cgcgatcaat ctgaagaaca ctttgttgtt ctatggaatc
       61 ctggagatcg gcattgctct ctttggcgtg gtcagtttca gtattattca atggattgga
      121 caaagcctgg cgggaagtcc atatgttctt gtgttcctga tcagttttct tgttttactg
      181 atccccacat ttctcatggg aatgactctg ccgctcctga cgcagtcgtt tgtggatcgg
      241 gtggagacgt ctggaagagt catcggcgtt ctgtatggta tcaacacact tggtgcagct
      301 attggatgtg cattggcagg atacattttt atagggttct tgggattgga cgggaccatt
      361 tacatcgcgg tgctattgaa tgccatcgtc ggtctgtgcg cgtttttctt atcaccctgg
      421 caaaggacag agattataaa gtctgaatct gttccaacaa cggatatttc aaataacgtc
      481 ctgggatatc ggaccatttt gctttcgtcg tttttagttg gctttcttgg actcggattc
      541 gaaattctct ggatccgagt cctgatgatt gtcaataaga acactgctta tagctttccg
      601 tctattttat ttatattcct gttgggattg gcaatcggcg gagcgatctg gggacggaag
      661 gcagacacat cttccaaccc tgcgcagtta ttttgcaaga tcgaaatggc tggcgcggct
      721 gtggctgttt tcacgtttct tgggttctgg atatcagttc agggggatgc ctcatggctt
      781 ggaaattttt tcgaaaccca aaaaccgagt cttccctttc ttcgaattga tcgcgagtgg
      841 tccttttcca aaatggcatt ggtggccaat ctctggagtt actttctacc catcttgatc
      901 ctggtgcttc cggcaaccct tgtgctgggt ggaggcttaa ctattctgga caggctatct
      961 atcaataatc cgcttgtatc cgggcaacgg gtaggagata ttcaccttgc gaatatcatc
     1021 ggctctgtgg cagggacctt cgtcaccagc tttatcctac tcccgggcat cgggtcagaa
     1081 tggacattga agctgttcgc gctttcgaca tttttattcc ccgcctttta ttttctggat
     1141 accagaagca aaccaactgt tcaaaacctt tcttcgttaa tggtgattgg ttcagtattg
     1201 ctcatcggga ttatccttct accaagcagg ggagagttct acacgcggct ttatgctgct
     1261 ggttcggaac aggaagtcgt cgtttcagag tcgggtgaca gcgtccttgc cttgacgtac
     1321 gagtcgcgcg gcatcaatga aaaaggtctg ctctggatcg gaggtgagat caacagcttc
     1381 tttccgccag agggattata tgaatcaaga gcgatggttt gtgccggtgc atccacccca
     1441 aaacgaattc tcgtcattgg cctgggcggc ggatacatta ccatgttctt caaaagtatt
     1501 cccaccgtgg aagagatcgt gattgttgaa ctccttgaag atctgtcacc atttctgacg
     1561 gaaaatcaag cctcgattcg tgagacactc gccgacccgc gtatcactta catcgttgac
     1621 gacggacgca ggtatctgaa tgcattcccc gatgagaaat tcgatcttat ttccattgac
     1681 ccgctgcgtg agcatactgc cgggcataac aatctctact caaagcaggc actcgaaatt
     1741 taccgaaacc atctcaccga aaatggcgtt ttatgcgcat ggatggcgga gcggcatatg
     1801 atcccgcatt cagtggcgca ggtgtttcca tacacagacc agttcgccaa cgagtatatg
     1861 attgccagca ataatcccat tcaatacgat cgcgcttaca tggaaaatgc ctccgctgac
     1921 ttcgatgatt tagcaaaatc tatatatggt tcggactttg ctgcaattcc ggatgtcgaa
     1981 cataacctga gttttttcct gcgcgatcag aatcaaatct tgattgagga atcaggcaca
     2041 cctgttcttg atgactcgca tcccttgtta gagtattttc ttttcagaaa acctgcaagg
     2101 agagagatta cacctctgcc tgatggtcag ctggaatttc aacgcaggct taattagcaa
     2161 tagatgaaca agcgcttcat cattcttgcg atatgctgtg ttttgatttc aggttgtgct
     2221 atgggtgtcg aagctgtaac gcccattgct gtttcagcaa ccgcaacgcc tgtcccagca
     2281 ggagatcttg gttttgggaa gattcatggc aaggtcaccg atgacgtcac aggcgcgcca
     2341 attgcaggag cagttattac ctgtgagcat cattccttta catctcccgc gacccgctcg
     2401 ggcaccgcga tgacggacgc aaatgggatg tataaattcg aaaacgtgta cttccatgat
     2461 acggatacga tcaaattgac ggtgcaggca accggatatc aaacgcagga aatctcgagt
     2521 gcatttttta gaatggcgga actggaagcg aatgtaactt tgaatcgcga tccataacca
     2581 taaagtgagt aaaatcccat aatgccatat tggaaactct attatcactt catttgggga
     2641 acaaagaaca ggtttccatt aattgactct gttctcgaac ctgaacttta tcgtgccatt
     2701 gctgccaaag cgcagaagtt ggggggattt gttcatgcaa tcggcggaat agaagatcac
     2761 gttcatctct cggtttctat tccaccaaag ttggcacctg caaaatttat tggtgatgtg
     2821 aaagggaata gttctcactt tgtaaattat gtgatcaaac ctgattttga atttcgctgg
     2881 caggctgagt atggcgtggt atcttttggt gaaagaaatc ttgcttcggt agggcgatat
     2941 attcataacc agaaacaaca tcacgcggaa gggacgttga tcgcggcgat ggagaggatg
     3001 gatgagcttt agtttgtgca gagcaactat gttcgcacgg tggtaaacca ccgtgctagt
     3061 cgtataaagc ccgctaaagc ggactaggtg caaggcaagg cggctttagt cgccttagta
     3121 ggcatagccc gacggttcat cgtcgggcgg acaatattgt gcgagtatcg aagctgatat
     3181 aagcagtgcc taacagtgga aaaactaact caaatgttct aaaaacctcc tgcagggtgt
     3241 aaaattgtag aaacctgtaa atgaatcctt agcgctcttc gcgtgctttg cggttaaaaa
     3301 ttatgatgaa ctttatccct cgaccctcgc aggaacaaat cctccgctat aacggcgggc
     3361 ggctgggcat tgccgcggtc ccgggcgcgg gcaagacgca gatcctctcg gcattagccg
     3421 cgcagatcat ctccagcggc gcgctcggcg acgaccagga agtgctgata gtcacgctgg
     3481 tcaactcggc ggtggataac ttcgaagcgc gcatcaaacg cttttttgat aatcctctcc
     3541 atgcgcttta taaatatcgc gtccgcacgt tgcatgggct ggcgcacgac atcgtccgtg
     3601 agaaaccagc gcgggtcggg ctcgaggagc ggttcgccat cctcgacgag cgcgaagcgg
     3661 acttcatccg ccgtgaggca gtcaacgcgt tcctatccac caatcccggt taccttgatg
     3721 actatctcga ccccgcgctc gatgaatcca aacgggactg ggtccgccgc cagcagctac
     3781 cagacatgat ggaaagcctg gtgctggcgt tcatccgttc tgcaaaggac aggcggctga
     3841 cacctgaccg aatccgtcaa ctgctggacc aggcacccgc ttcgcttccg ttggcagaaa
     3901 tgggattagc gatttattcc gactatcagc gtgcccttgc ttatcgcggc gccgttgact
     3961 tcgacgatct cattcgcttg tctcttgacc tgctcgagaa tgacgaggaa tacctggagc
     4021 gtttgcgtca tcgctatcca ttcatccttg aagatgaagc gcaggactcc agtgttaccc
     4081 aggaaaggat attgagtctg ttatcgggag aaggcggcaa ttgggtgcgc gtgggtgacc
     4141 ccaaccaagc catcttcgag acgttcacta cggcatcgcc agagctcctg cgcgaattta
     4201 ttcgtaacaa taaccatgta gacatgaacg aatcgggtcg ttcgcagcag ttcatcattg
     4261 accttgcgaa tcatctgatc gactgggtga tgaccgagca ccctgcctcg caggcccgga
     4321 acgcgcttga tacgccgtat atcgaacctg tccctgaaga tgacccgcag cggaatccgg
     4381 acgaagataa gtccgcgatt cgatttgcag agaagcgact cacccccgaa gaggaagttg
     4441 atgcagtagt caagtcgatt cagcggtggc tacccgagaa tacggatagg acggttgccg
     4501 tattggtgcc gcgcaacgca cgtggtgtgg aggtcattga cgcgctcaag aagaaaggga
     4561 ttgaggttat cgagttcctg gcgtccacat cgaacacgcg cgcggcggca ggcgcgttga
     4621 attatttgct gtcgtatctg gcagacccgc aatc
//
LOCUS       DASTJP010000529         7785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_175149, whole genome shotgun sequence.
ACCESSION   DASTJP010000529 DASTJP010000000
VERSION     DASTJP010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175149"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFQ23_15490"
     CDS             complement(<1..777)
                     /locus_tag="VFQ23_15490"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9908054.1"
                     /translation="MNISTEIIIIIVVILINAVFVLSEMSVASSRKARLQQRINEGDQ
                     RAETVLDLIENPNLFLATVQIGITLVGVFVGAVGGATLSEPLSVLFQSVPWLADYAET
                     AALAIVVVTITFFSIVLGELVPKRIALHNPELISSTLAGPMIVVSKIFAPFVWLLGRI
                     TDFILKILGVQPGNEPPVTEEEIQLLIDQGTQAGVFEEAEHDMVEGVFSLGDQRVYSL
                     MTPRTDVVYLDVNDSIEVIREKIAQSEFSRFPVRQGTLDVI"
     gene            complement(965..2023)
                     /gene="selD"
                     /locus_tag="VFQ23_15495"
     CDS             complement(965..2023)
                     /gene="selD"
                     /locus_tag="VFQ23_15495"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1961..1963),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET9908055.1"
                     /translation="MPIPKTGREIRLTTLSACAGUASKLSADALTQVLRPVKDIFDAD
                     SFPDLLIGLREPDDAAVWRLDEARAIVVTTDFFTPVVDDPYDYGSIAAANSLSDVYAM
                     GGQPFLALNVAALPDDLPVEISSEIIRGGAEKAREAGVVIAGGHTVKDKEPKYGLVAI
                     GFVDPRNMLSKGGLSIGDELVLTKPLGFGVTTTALKREQADEQDVAEAVDWMKRLNKT
                     ASQLAVEFELRGGTDITGYSLLGHGLEMAEASGVSLKLESANIPLISCARKYAEKGSF
                     PGGAFDNKKYFESKVNFANSVDEPNQMLLFDPQTSGGLLLGVPQSKLDAFLSRAKETD
                     QQLWRIGEVQAGKGIKVI"
     gene            complement(2091..3143)
                     /locus_tag="VFQ23_15500"
     CDS             complement(2091..3143)
                     /locus_tag="VFQ23_15500"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HET9908056.1"
                     /translation="MNIVDVIRTLAGFAWLAAIGLGILAVIRASRNQSAKATSTTVIV
                     MLIAAAILTTLGAGLVYVESSERGVVRTIRAGGVRPEALGPGLHWIIPVVEQVVTYSI
                     SNQTYTMSVAPQEGQVSGDDSIRARTKDGQEVIIDASVIYQIDPTKVVPLHVAWQNRY
                     EDGFVRPEARGIIRDVVSQYGVEEVVSTKRAEMVQIISDTLGGSMAENNLRLIDFVLR
                     DIHFSEAYAVAVEQKQIAEQQALQAEFVVESKRQEAEQARQIAQGQADAAVIAARGAA
                     EARLIEAEAEAQALELLAAALESNPQLIEYQYILKLAPGVQTIFVPSDNQFILPLPDT
                     SSSPETPTIIPPTDSP"
     gene            3196..3873
                     /locus_tag="VFQ23_15505"
     CDS             3196..3873
                     /locus_tag="VFQ23_15505"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908057.1"
                     /translation="MQTGKGQVVELILQDGLRHARLNCPENLVPAPGQYLLVGHDPSD
                     PLPVPIFSTDSVPNGFIVAPPIPESWSPGRVVNLRGPLGKGFSLPSSAKRVALVAIDI
                     SPSRLSGLIRPALLQEASVVVVSDLSVLSLPDEVEIQPLSALNEVAKWADYIACDVAR
                     EGISPLRDRLIGEKPSKVSFRAQVLVRTPIPCGGLAECGVCAVTSRSGWGMACKDGPV
                     FDWTELI"
     gene            complement(3915..4856)
                     /locus_tag="VFQ23_15510"
     CDS             complement(3915..4856)
                     /locus_tag="VFQ23_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908058.1"
                     /translation="MRAGRFVISFLALTILLAVVGQAKAQSADVKYVPETGHNIRGDF
                     LRFYNRAKDPRLVYGYPITEQIKSKDGKTVQYFQRARFELTSDGLGNPRILLTSIGQA
                     AYQPGGQLNIHNSSGCQLFSSGYSICFTFLDFYKDNDGASQFGNPISPFEYRENLIVQ
                     YFERARFEWRADRPEGQRVVLTDLGRTYFDLIGEDPVHLKSAAPLDATINPVLSLRVR
                     AFVQKSIVLGSGDQTVFVVVQSQTLQPVSNAAGKATINWPNGQTQDYYFNTNSAGIGS
                     FKFTFTDQKQGELVPIDILVTYQGLVGKTATSFRIWY"
     gene            complement(4923..6290)
                     /locus_tag="VFQ23_15515"
     CDS             complement(4923..6290)
                     /locus_tag="VFQ23_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET9908059.1"
                     /translation="MSFQKICVIGLGYIGLPTAAIFAANGLKVIGVDVNPDVIETLRS
                     GEVHIHEPGLRDLFKRSLLSGNFTFSTEPEEADAFLIAVPTPFQTSAYREHNGRKYKL
                     ADLQAVRSAAEAIVPCLRKGNLVILESTSPPRTTIDVVRPILETSGLRAGDDFFLCYS
                     PERVLPGQILRELTENARVVGGITPDSAKAGHDLYRTFVKGEIVETDTTTAEMVKLME
                     NTTRDINIAVANEFSRLAEYFGVDIWEAIGLANRHPRINILSPGPGVGGHCISVDPWF
                     LVEAAPELSSLIYQARQVNEAQPHFVVEKVKQSLAQVDSELGRSNHRDTLKGKKIAAL
                     GLAYKPDVDDLRESPAIEVVRLLREMGADVVAWEPFKPNANLTGIEMATSLDGAISDA
                     DAILLLVRHTEFKNFNPGDIASKTTARIAIDTVNAWDGTEWEKAGFQLYRLGTGQLPI
                     QKTPS"
     gene            6475..7077
                     /locus_tag="VFQ23_15520"
     CDS             6475..7077
                     /locus_tag="VFQ23_15520"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET9908060.1"
                     /translation="MDFLLEPNIAYLFLLGGVLMAMLALATPGTGFLEIGAFFCIVLA
                     GYAVYNLSYNWWALLLIVLSIVPFIYAIQKPKRELFLGVSILLLIIGSVFMFPRGAEH
                     AGVNPAVATVASGLTAGFLWIAIRKSMEAAYARPRHDLDGLVGQVGEARTVVHDEGSV
                     QVGGELWSARSESSIPAGSSIRVVRRDGFIVVVEKVNHTN"
     gene            7088..>7785
                     /locus_tag="VFQ23_15525"
     CDS             7088..>7785
                     /locus_tag="VFQ23_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9908061.1"
                     /translation="MDPLGLGSGLCFVGGLVVLVFIFLANAIRIVPEYQRLVVFRLGR
                     SIGPKGPGIVLLIPVIDRAVKVDLREQVREVPHQTSITKDNAPISIDFLWYYKVLDPA
                     ESVLQVGNFESAAAGIAATTLRAVIGGIPLDDVLSEREHINNMLRTRLDEVTERWGVK
                     VTNVEIREIIPPRDVQDAMNRQMSAERIRRAVVTESTGTREAAVNVAEGEKQSNILRA
                     EGQKQSAILAAEGE"
BASE COUNT         1884 a         1918 c         1915 g         2068 t
ORIGIN      
        1 aattacgtcc agtgttccct ggcgcacggg gaagcgtgaa aattcacttt gcgcgatctt
       61 ttctcgaatt acttcgatgg aatcgtttac atccaaataa acgacatccg tgcgaggggt
      121 catcaacgaa tagacgcgct ggtcgcccag gctgaatacg ccttcgacca tgtcatgttc
      181 ggcttcttcg aaaacacccg cctgtgtgcc ctggtctatc aacaattgaa tctcctcttc
      241 agtgacagga ggttcattgc cgggttggac gcccaaaatt ttcagaatga aatctgtaat
      301 tcggcccagc aaccagacaa atggtgcaaa tattttcgaa acgacgatca tgggccccgc
      361 cagagtagat gagatcaact ccgggttgtg taaggcaata cgtttaggga ccagctcgcc
      421 cagaacgatg gaaaagaacg taatcgtaac aaccacgatg gccaaggctg ccgtttcagc
      481 ataatccgcc agccagggca cactttggaa gagcacactc aaaggctcag agagcgtggc
      541 cccgcccacg gcgcccacga agactcctac aagggtgatg ccaatttgta cggtcgcgag
      601 aaatagattt ggattctcga tgagatcaag aaccgtctct gctcgttgat ccccttcgtt
      661 tatacgttgt tgaaggcgtg ctttgcgtga tgaagcaact gacatttcag acaaaacaaa
      721 taccgcattg atcaggatca caacgatgat aatgatgatt tctgtactga tattcatcta
      781 aattaatatt attttctgct tgaggactta acgaatctca atcagccagg ttactccata
      841 cctggataaa cctccgttgg gaggggtgtt ataaatgctg acgaattact gtctatgggg
      901 acgtccggca tgatttcgtg aacttctcta ctctccctgt attgtactat ggataacgcg
      961 ttagttagat aacttttatg ccttttcctg cttgaacttc tccaatccgc caaagctgtt
     1021 gatcggtttc tttcgcacgc gagagaaagg cgtcaagttt ggattgtgga actccaagga
     1081 gtagcccgcc gcttgtctgc ggatcaaata atagcatctg atttggttca tcaactgaat
     1141 ttgcaaagtt tactttcgac tcaaaatatt ttttattatc gaacgcgcca ccaggaaaac
     1201 ttcctttttc ggcatacttg cgcgcgcagg agatcaatgg aatatttgcg gattccagct
     1261 ttagtgagac gccggaagcc tctgccattt ccagcccatg acccagcaag ctatatcctg
     1321 taatatccgt tcctcctcgc agttcaaatt cgactgcaag ttgactcgcg gtcttgttca
     1381 gtcgtttcat ccagtctacg gcttctgcta cgtcttgttc atctgcctgt tcgcgtttta
     1441 gcgcggtggt cgtaacgcca aatcccaggg gctttgtcag cacgagctcg tcaccgatgc
     1501 tcaatccgcc tttactgagc atgttgcgtg ggtcaacaaa accgatggcg accagcccat
     1561 attttggctc tttgtctttg accgtatgtc cgcctgcaat gactacgcct gcttcacgcg
     1621 ccttctcagc gccaccgcgt atgatttcgc tcgagatctc aacaggcaga tcatcaggta
     1681 gtgctgcgac gttcaaagca aggaaaggtt gcccgcccat cgcatacacg tcagacaggc
     1741 tgtttgctgc cgcgatcgag ccgtagtcat agggatcatc aaccaccggc gtgaaaaaat
     1801 cagtggtcac cacaattgcg cgcgcttcgt ctaaacgcca aactgccgcg tcatcaggtt
     1861 cccgcaatcc aattaaaagg tcggggaatg aatcagcgtc aaaaatgtct ttgacaggac
     1921 gcagtacctg cgtcagcgcg tcagcgctta gtttggatgc tcaacccgca caggcagaaa
     1981 gggtcgtcag gcggatttcg cgtccagttt tgggaattgg catcttcctc caaaaaaaac
     2041 accagcaacc agtatttctg gtcactggta ctaggcgatt tttgatacgg ttacggtgag
     2101 tctgtaggcg gtattatcgt aggtgtctca ggtgacgagc tggtatcagg caaaggcaaa
     2161 ataaattgat tgtcactggg tacaaaaatc gtttgtacgc caggcgcgag ttttaagatg
     2221 tactgatatt caatgagttg tgggttactt tcgagcgccg ctgccaacaa ttccaatgcc
     2281 tgtgcttcgg cctctgcttc gattaggcgt gcctctgctg cgcctcttgc cgcaatcacg
     2341 gctgcatcgg cctgaccctg tgcgatctgc cgcgcctgtt cggcttcctg tcgcttggac
     2401 tcaacaacaa actcggcttg tagcgcctgc tgttctgcaa tttgcttttg ttcgacagcc
     2461 acagcatacg cttcactgaa gtgtatgtcg cgtaaaacga aatcaatcag tcgcaaatta
     2521 ttttccgcca tgctgccgcc caatgtgtct gaaatgatct gaaccatttc tgcgcgtttc
     2581 gtcgaaacaa cctcttccac accatattgc gagaccacat cacggatgat cccgcgcgct
     2641 tcagggcgca caaatccatc ctcataacga ttttgccacg cgacatgaag cggtacaacc
     2701 ttggttggat ctatttgata tattaccgat gcgtcaatga taacttcctg accgtccttc
     2761 gtacgggccc gaattgaatc atcaccggac acttgcccct cctgaggtgc aacagacatc
     2821 gtgtaggtct ggtttgaaat tgaataggtc accacctgct caacgaccgg aataatccaa
     2881 tgcaagccag gtccaagtgc ttcgggtcgt acaccgccag ctcgaattgt tcgtaccaca
     2941 cctcgttcac tcgattccac atataccaaa ccggctccaa gtgtcgtcag tatcgctgcg
     3001 gcgatcaaca taacgattac agtggtactc gttgcctttg cgctttggtt acggcttgca
     3061 cggataacag ccagaatgcc caatccaata gccgccagcc aggcgaagcc agccaatgta
     3121 cgaatgacat caacgatgtt catggttcct ccaaatcagg ttgaaataat tataccgcca
     3181 tgatagcata gcgatatgca aacagggaaa gggcaggtag ttgaattgat ccttcaagac
     3241 gggcttcgtc acgcgcgtct gaattgccct gagaatttag ttccggcgcc gggacaatat
     3301 ctgctggtcg gacacgaccc atctgaccct ttacctgttc ctatcttttc tacggattct
     3361 gtgccaaatg gttttattgt cgcgccaccg attcctgaat cctggagccc gggtcgagtt
     3421 gtcaacctgc gcggtccact ggggaagggg ttttcactac cctcatccgc aaagagggtt
     3481 gcattggtgg ctattgatat ttccccttcg cgcctcagcg ggttgattcg ccccgcgcta
     3541 cttcaagaag cgtctgtggt ggtcgtaagt gacttaagcg ttcttagctt gccagatgaa
     3601 gtggagatcc agcccctttc agccttgaat gaagttgcca agtgggcaga ttacatagca
     3661 tgtgatgtgg cgcgagaggg aatttcacca ttacgcgata ggttgattgg cgaaaagccc
     3721 tcaaaagttt ccttccgagc ccaggttctg gttcgcactc caatcccctg cggcgggttg
     3781 gctgaatgcg gagtctgcgc ggtgacttcc agatctggtt ggggaatggc gtgtaaagac
     3841 ggacccgtgt ttgactggac ggaactgatc taaacacttc ggggagagtt cgcgcgggcc
     3901 aggtggaatg aggattagta ccagattctg aacgaggttg cagttttccc cacaagccct
     3961 tgatatgtga ccagaatatc aatcggcaca agttctcctt gtttttgatc tgtaaatgta
     4021 aatttgaaac ttccgatccc cgcactattt gtgttgaaat agtaatcttg cgtctgacca
     4081 tttggccagt taatggttgc tttaccggct gcattcgaca caggctggag agtctggctt
     4141 tggacaacta caaatacagt ttgatctccg ctgccaagca caatcgattt ttgtacgaag
     4201 gcgcgaactc ttaacgataa cactgggttg atcgtcgcat ccagtggagc tgcagatttg
     4261 agatggactg ggtcttcgcc aataagatca aagtaagttc gcccaaggtc ggtaaggaca
     4321 acgcgctggc cttctggccg atctgcacgc cattcaaagc gtgctctttc aaaatactga
     4381 acaatcagat tttcgcgata ctcaaatggc gatatcggat tcccaaattg cgatgcacca
     4441 tcattatcct tgtaaaagtc cagaaatgta aaacaaatcg agtacccaga ggaaaacagt
     4501 tggcaacccg acgagttgtg gatgttcagc tgtccaccgg gttgatatgc agcttgtcca
     4561 attgaagtaa gcagaattcg aggattgccc aaaccatcac tggttaactc aaagcgggca
     4621 cgttgaaagt attgaaccgt tttgccatct tttgacttaa tttgttcagt gattgggtaa
     4681 ccataaacaa gcctggggtc tttggctcta ttatagaatc gtaaaaaatc ccctcgtatg
     4741 ttgtgtcctg tttccgggac gtacttcaca tctgccgatt gagcctttgc ctgtcccacc
     4801 actgccagca gtattgtcag agcaagaaat gaaataacaa atcgtccagc tctcatcaga
     4861 ttcattatac tcatatcaag caggctaaat gagggatgca ccttccaatt tggataggaa
     4921 tttcaagacg gcgttttctg tatgggtaat tgtcctgtac caaggcggta taactggaaa
     4981 cctgcttttt cccattcagt accgtcccat gcattgacgg tgtcgattgc tatgcgcgcc
     5041 gtcgtttttg aagcgatgtc gcctgggttg aagtttttga actctgtatg cctcactaag
     5101 agcaaaatgg catctgcgtc gctgatagca ccgtccaggc tggtcgccat ttcaatgccc
     5161 gttagatttg cattgggttt aaaaggttcc catgctacta catctgcacc catttcgcga
     5221 agaagccgaa ctacttctat cgcagggctc tcgcgcaaat catcgacatc gggtttatat
     5281 gccaaaccca acgcagcgat ttttttacct ttcaaagtat ccctgtggtt gcttctaccc
     5341 agttcagagt cgacttgtgc caaggactgt tttacttttt cgacgacgaa atgtggttgt
     5401 gcctcattta cctgacgtgc ctgataaatg agtgacgata attcgggtgc agcttccacc
     5461 aggaaccatg gatccacgct aatgcaatgg ccaccgactc caggccctgg gctgagtatg
     5521 ttaatgcgcg ggtggcgatt ggcaagtcca attgcctccc atatatccac accaaaatat
     5581 tccgccagac gcgaaaattc atttgcgaca gcaatgttga tatcgcgggt cgtgttttcc
     5641 atgagcttaa ccatttccgc ggtggtggta tccgtttcaa caatctcgcc tttcacaaag
     5701 gtccgataga gatcatgacc ggctttggca gaatctggtg taatgccccc cacgacacgc
     5761 gcgttttcag taagttcacg taaaatctgc ccgggcagta cccgttcagg cgaataacaa
     5821 agaaagaaat cgtcgcctgc cctcagcccc gaagtttcaa gaattggtct gaccacatcg
     5881 atcgtagtgc gcggagggga ggtagattcc aggataacca gattgccttt ccgaaggcat
     5941 ggaacaattg cttccgctgc tgagcgtact gcctgtaaat ctgccagctt atattttctg
     6001 ccgttatgtt ctctatatgc acttgtctgg aagggagttg gtacagcgat cagaaacgcg
     6061 tcggcttctt caggttctgt tgaaaacgta aaatttccag aaaggaggga ccgcttaaaa
     6121 agatcacgca gacccggttc atgaatatgg acttccccac ttcggagggt ctcaatcaca
     6181 tcgggattga catcgacgcc tatgacttta agcccattgg cggcaaaaat ggcggcagtt
     6241 ggcaacccga tgtagccaag cccaatgaca cagatttttt gaaaactcaa tttgctcctt
     6301 acttttcgcc tcccacggtt tcaaagagtc tgttggaggt ggatgaaaat tgatgagata
     6361 cggtccggct cattataaag ccttggcgga aaatctttag gcaactttaa tgcttgaatt
     6421 ttcgtagaat gaatcagaat ttttcgtctt gttgtataat gacttggagg agttatggac
     6481 ttcttacttg aacccaacat cgcgtatctt tttcttctgg ggggcgtctt aatggcgatg
     6541 ctggcgcttg ccacgccagg gacaggattc ttggaaatcg gtgcattttt ctgtattgtg
     6601 ctagctggct acgcggtcta taacttgtct tataactggt gggcgctgct gctcattgtt
     6661 ctgagtattg ttcccttcat ttatgcaatt caaaaaccga agcgagaact atttttaggg
     6721 gtatcaatcc tcctgttgat catcggttcc gtattcatgt ttccgcgagg ggcggaacac
     6781 gcaggagtca atccggcggt ggccactgtg gcttcgggct tgaccgccgg ctttttatgg
     6841 atcgcgattc gaaaatcgat ggaggccgcc tatgcgcggc ccaggcatga tcttgatggc
     6901 ctggtagggc aggtcggaga agccaggaca gtagtccacg atgagggaag tgtgcaagtc
     6961 ggaggtgaat tgtggtcagc tcgcagtgaa agttctatcc cggccgggag ttcgattcga
     7021 gtcgtgcgta gagatggttt tatcgtcgtt gttgaaaaag tgaatcatac caattaagga
     7081 gattgacatg gatccattag gattaggtag tgggctttgt tttgtcggtg ggcttgtcgt
     7141 cctggttttt atctttctgg cgaatgccat tcggatcgtt cctgaatatc agcggcttgt
     7201 cgtgtttcgt ttaggacgca gtatcgggcc aaagggacca ggtatcgttt tgctgattcc
     7261 ggtgattgac cgcgctgtaa aagtagactt gcgcgaacaa gtacgcgagg tgcctcatca
     7321 gacttcgatc acaaaagata acgcgccgat ctcgattgac tttctctggt attacaaagt
     7381 gctcgacccg gctgaatcag tattacaggt tggtaacttt gaatcagctg ccgccggtat
     7441 tgcggctacc acactccggg cggtcatcgg tggaattcca ttggatgatg tgctttctga
     7501 acgcgaacac attaacaata tgcttcgcac acgcctcgat gaagtgactg aacggtgggg
     7561 cgtaaaagtg acaaatgtgg aaatccgcga gattattcca ccgcgcgatg tgcaggatgc
     7621 catgaaccgg cagatgtctg ctgaacgcat ccgtcgcgcg gttgttacag aatcgactgg
     7681 tacgcgcgaa gccgcagtca acgtggcaga aggcgaaaag caatccaaca ttttgcgggc
     7741 agaaggtcaa aaacagtctg ccatcctcgc ggctgaaggc gaaaa
//
LOCUS       DASTJP010000530         8482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_903791, whole genome shotgun sequence.
ACCESSION   DASTJP010000530 DASTJP010000000
VERSION     DASTJP010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903791"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /gene="coaBC"
                     /locus_tag="VFQ23_15530"
     CDS             <1..975
                     /gene="coaBC"
                     /locus_tag="VFQ23_15530"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET9908062.1"
                     /translation="IAKLAHGQSDTLLTVTTLASTCPLMIAPAMDGGMFDHPATQENI
                     EILEKRGTVIVGPAEGHLASGLSGKGRMLEPAELIGHARLVLGRNGSLAGKRIVITAG
                     GTQEPLDPVRVLTNRSSGKQGYALAQAALDMGAQVTLITTPTSLTMPMGAKAIRVETA
                     KQMLEAVLKESEISDALIMAAAVADFRPKNKAENKIKKETGIPHIELEATEDILKTIA
                     GQRAERIALREGTDTRIQEDKAKHPRVVVGFAAESQDLLQNATNKLESKGLDFIAAND
                     ISANDAGFAVETNRVTLLFADGRTESLPLMSKTQTAEIILERVAILLE"
     gene            977..1708
                     /locus_tag="VFQ23_15535"
     CDS             977..1708
                     /locus_tag="VFQ23_15535"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9908063.1"
                     /translation="MRILVISDIHANYTALKAVLNDAGTVDETWCLGDLVGYGPDPNA
                     VIEEVREIPNLTCLLGNHDVAVIGKIPFEAFNGDARRTLEYHERVLTADNMDFLRSLP
                     FNLKVREGASIVHGSPRDSVWEYILNTLSARLNFDHFTTPWCFVGHSHLQCMFHLDEK
                     TDRVNLQPIRVGEAMQLQPRLIINPGSIGQPRDRDPRAAYAIYDSTERTWEPRRVEYN
                     IPEVQKRIREAGLPEKHAVRIAEGW"
     gene            1850..2848
                     /locus_tag="VFQ23_15540"
     CDS             1850..2848
                     /locus_tag="VFQ23_15540"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET9908064.1"
                     /translation="MKRPIFIILSVITIFILLGLSVTGLFGRRVGQTFSTINNQLPGF
                     GGGSDPQFSYSEAPAEAPMPATDLFSAEEAARQSVDGSVTNQQGQDRLVIKNADLAVV
                     VKDPAARMKEISAMATEMGGFVVSSNLYQSYYGPNSVEVPEATVVIRVPSERLDEALE
                     RIKAGAVDIDHENVSGQDVTSQYVDLQSQLKAKQAAEQKLLEILDQATRAEDVLAIYL
                     QVQSVQSEIEVLKGQIKYFEESAALSSISVRLIAEESAQPITVGPWTPGGAAKQAIED
                     LIRFAQNFVDFLIYFVLSILPSLILIAIPLYFVFIGGRAVYRRVSKSKANVDEEKK"
     gene            2911..3621
                     /gene="rsmG"
                     /locus_tag="VFQ23_15545"
     CDS             2911..3621
                     /gene="rsmG"
                     /locus_tag="VFQ23_15545"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET9908065.1"
                     /translation="MEKLVRETQALFNIQLTEKQVTSLRMYEKELMEWNQKFNLTAIR
                     DAESIRTKHFLDSFSCILAWTTSPPDHLVDVGTGAGFPGLPLKILYPDLKLTLVESVG
                     KKAKFCQHVVEKLGLDDVKVIQTRAEEIGQDSIHREQYDWSVARAVANLTVLSEYLLP
                     LVKVGGAMLAQKGESGPAEAQSAEQAIALLGGKLKQLIPVKLPGVTEERYLIVVEKID
                     ATPSKYPRKPGIPAKQPL"
     gene            complement(3632..4195)
                     /locus_tag="VFQ23_15550"
     CDS             complement(3632..4195)
                     /locus_tag="VFQ23_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908066.1"
                     /translation="MKEKIRTIVSVILIVVVSTILLTDTTGRVDAILTFIKTYRVILV
                     IGFIFLFIAIFVISIWIGSIISGEAAALKKHPKFFEEMQAVLQNTLIPLGYKEDVGPS
                     GGLGRYVNYSRGESSVKLRLDVRDSIYYLNVASSRKTTTVKNDVIGEYELPLWDFSVE
                     GHIAEADRFKNDSITKLNKWLFEQGIK"
     gene            complement(4244..4606)
                     /locus_tag="VFQ23_15555"
     CDS             complement(4244..4606)
                     /locus_tag="VFQ23_15555"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9908067.1"
                     /translation="MSVRHSSDVEAKNVAAGKDTTIQVLISAQEGPNFALRKFSMQKG
                     GGMPRHTNKLEHEQYVLRGEATITIDDETHHVKTGDVVFIPEGAIHSYQNTGEEPFEF
                     LCIVPNKEDTITIVDEAC"
     gene            complement(4625..5719)
                     /locus_tag="VFQ23_15560"
     CDS             complement(4625..5719)
                     /locus_tag="VFQ23_15560"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET9908068.1"
                     /translation="MIRTVRSHLNVEALSHPGMTGKNNEDRYAVASFQVDDHNKMPVL
                     FAVLADGIGGHRGGEVAAELAVNHIMQTVAKSDTRHIRQTMEDAVTEASDAVAAHAAS
                     NEELRGMGATCATAWIVGDQLHTAYVGDSRIYLMRAGRIQQLTIDHTWVQEAMEKGIL
                     TPEQARTHPNVHVIRRYLGSSEPPVPDFRLRWFDGDDDVHLENNQGIQLLPNDVVLLC
                     TDGLTDLVWDDEILEIVRSKPSLKEASRALVELANSRGGHDNTTVILVSVPSNFKLSV
                     GKNMGWLPWVIGGVTGVMFVLVVGSFLTVSLLRRNANLTPTQTPTLPLLFTQTPLVQP
                     TLPAPLPTMTTLPLPIAPTYTPWPTNTGIP"
     gene            complement(5722..7635)
                     /locus_tag="VFQ23_15565"
     CDS             complement(5722..7635)
                     /locus_tag="VFQ23_15565"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9908069.1"
                     /translation="MSKFLTYILSLSLLAVPFTSARAQTAAYAEIASVDAQNFPQIST
                     LVDVYNANGEFMTGLRASDFTVYEDGEPRNADAISESNVPVQIVVAINPGPALAVRDA
                     NAVPRFTYVVDMLGRWADAQPADSQDDLSLVSLSGSLITHATAKDWFVSLGSFKPDFR
                     STTPNLQTLSIALDTVTAQSRQPGMKRAILLITPHMDDAGIDSTIAPLIQRAIDSKVR
                     VFVWFVDAPEYSVTPSGNAFKSLAQQTNGSFFAFSGVEPFPDPNQFFSPLRHVYNLTY
                     TSALKTAGDHTLGMYVEAPEGRIPALDKPFSVDIQPPNPIFVSPPLQITRQPAEDDPY
                     NSDALVPAQQAVEIIVEFPDGHERELKRTTLYVDGQAVTENISAPFESFMWDLSAYSK
                     SGQHEIIVEAEDVLGISKSSIGIPVTLTIVQPPRGIRAFLARYRSYLVIGAIALAGLA
                     LLGILLRGRAGTATPRSRRETRKQFEDPLTQPVVALTEPPISATKKTKTEPRSLRSGW
                     LQPKPARVAEAPAYLIRLTNGGEPASVSPIPVQEKDMTFGTDPVQSKRVLDDASISPL
                     HARIRQIDDGTFIIYDHGSVAGTWVNYEPVMRDGRRLTHGDRIHFGQLVYRFDLSRPP
                     AASEPKVITKKQV"
     gene            complement(7628..>8482)
                     /locus_tag="VFQ23_15570"
     CDS             complement(7628..>8482)
                     /locus_tag="VFQ23_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908070.1"
                     /translation="FNSNSFQGNGGPGTSPDLPPQFTEFFQWVEQNLTAFLVGTVAIV
                     CLIWIVTIFLSTIGKVGLIRGTAQIDGGAEGLIFGQLFSESTPYFWRMLGLSLILSLP
                     VFIFAMIIAVFAIFGLAASQGSDASGLGFFATFPLLIGCVCLLVPVMLVLGVIFRQAE
                     NALVLEEMGVLPALSRGWEVFRNNLGPVIIMAIILAVIGFAAGFVLALPIFVILVPAF
                     IAVMAGEAQSMTPLAIAGVCICLYFPVSLVLSGIAIAYTESAWTLMYMRLTKPQIAEP
                     VILPEANE"
BASE COUNT         2237 a         2136 c         2112 g         1997 t
ORIGIN      
        1 atcgccaaac ttgcgcatgg tcaatcggat acgttactaa ctgtcacgac gctggcatcc
       61 acttgtccgc tgatgatcgc gcccgccatg gacggcggga tgtttgatca cccggcgaca
      121 caagagaata ttgagatatt agaaaagcgt ggcacggtca ttgttggtcc ggcagagggt
      181 cacctcgcct ccggattatc tggaaagggt cgtatgcttg agccggctga gcttattggt
      241 catgcccgac tcgttcttgg acgaaacggc tcacttgccg gtaaaagaat cgtcatcaca
      301 gccggtggga cgcaggaacc gcttgacccg gtccgcgtct tgaccaaccg ttcctccgga
      361 aagcaaggct acgccctggc acaagccgcg ttggacatgg gcgcgcaggt cacgttgatc
      421 acaacaccaa cgtcgctcac catgccaatg ggtgcgaaag cgattcgggt cgaaacagcg
      481 aaacaaatgc ttgaggctgt gctaaaagaa tcagaaataa gcgatgcact tattatggct
      541 gctgctgtgg cagattttcg accaaaaaat aaagcggaga acaagatcaa aaaagagacc
      601 ggtattccgc acatcgagct tgaagccaca gaagatattt tgaaaacaat agcgggccaa
      661 agagcagaaa gaattgcact aagagaaggc accgacactc gtatccagga agacaaagct
      721 aaacatccgc gtgttgtggt tggcttcgca gctgagtcgc aggacctcct tcaaaatgca
      781 acaaataagt tagaatccaa aggactggat ttcatcgctg caaatgatat ctctgccaat
      841 gacgcaggct tcgcagtcga aacgaatcgc gttacactat tattcgcgga cggacgaacg
      901 gaatcattgc cgctgatgag caagacacaa acagctgaaa ttattttgga acgggttgca
      961 attttgttgg agtaatatgc gtattctggt catttcggac attcacgcga actataccgc
     1021 actgaaagcc gtactaaacg atgcaggcac tgtagacgaa acctggtgcc tgggggatct
     1081 ggtaggttac ggacccgatc caaatgccgt gatcgaagaa gtccgcgaga ttcccaacct
     1141 cacttgcctg cttggcaatc atgacgtggc agtgatcggc aagatcccct ttgaggcgtt
     1201 caatggagac gcgcgccgaa cccttgaata tcatgagcgc gttctgactg cagataatat
     1261 ggattttctg cgttccctcc cgttcaacct gaaggtacgt gaaggggcaa gcattgtgca
     1321 tggcagccca cgcgattcgg tctgggaata tattttgaac acactttcgg cgcgcctgaa
     1381 cttcgatcac ttcacgacgc cctggtgctt cgtaggtcat tcccatttgc aatgcatgtt
     1441 ccatctggat gagaaaactg accgcgtgaa tctgcaaccg atccgggtcg gcgaggcaat
     1501 gcagctgcag cctaggctga tcatcaaccc gggttccatc ggccagcccc gtgaccgtga
     1561 cccgcgcgcc gcctatgcta tttacgatag cacagaaaga acatgggagc ctcgccgcgt
     1621 ggaatataac attcctgaag tccaaaagcg tattcgcgag gcaggcttgc ctgagaaaca
     1681 tgcggtaaga attgctgagg ggtggtagcc ccctccgcca ctctgtgaca tctctcctat
     1741 tttctaaatg aagttcgccg gaaatgggtt gggttagggc tgggaacatt tatgaagagc
     1801 aaggcgtcct tcgggtggaa tcactttact tttatcaagg agctacataa tgaaacgccc
     1861 aatcttcatc atcctttcgg tcatcacaat ttttatcttg ctcgggctat ccgtgacggg
     1921 tttatttgga cgcagagtcg gacagacctt cagcaccata aacaatcagc tgccaggttt
     1981 tggcggaggg tcagatcctc aattcagcta cagtgaggca ccggcggaag cgccaatgcc
     2041 agccactgat cttttttcag cagaggaagc agccaggcaa tcggtcgatg gttcggtgac
     2101 aaatcagcaa ggccaggacc gactagtcat caagaatgcg gatctggcag tcgtggttaa
     2161 agatcctgca gcgcgcatga aggaaatctc tgccatggca accgagatgg gcggctttgt
     2221 tgtctcatct aatttatacc agtcgtatta tgggccgaac agtgtcgaag tgccagaagc
     2281 aaccgttgtt atccgtgtgc ccagcgaacg cctcgatgaa gcccttgagc gcatcaaagc
     2341 aggcgcggtg gatatcgatc atgaaaacgt cagtgggcag gatgtgacca gtcaatatgt
     2401 ggatttacag tcacagctca aagccaagca ggccgcggag caaaaattac tggagatcct
     2461 ggatcaggcc accagagcag aggatgtact tgccatttac ctgcaggtgc agagtgttca
     2521 aagtgagatt gaagttttga agggtcagat caaatatttc gaagagtcag ctgcgctatc
     2581 atccattagt gttcgcctga tcgctgaaga aagcgcccaa cctatcacag tcggtccatg
     2641 gacacccggt ggagcagcca agcaagcgat tgaagatttg atccgctttg cccagaattt
     2701 tgtggatttc ctgatctact tcgtgctgtc cattctgcct tctttgatcc tgattgccat
     2761 cccgctttat tttgtcttta tcggtgggcg tgccgtgtat cgcagggtta gcaagtcaaa
     2821 agccaatgta gacgaagaga agaagtaatt cgaaatttac cctcacccct gcccctctcc
     2881 caaataggag aggggttata atttttaaaa atggaaaaac tcgttcgcga gacgcaggca
     2941 ttgttcaata ttcagctcac tgagaagcag gtcacatcgc tgcgtatgta tgaaaaagag
     3001 ctaatggaat ggaatcaaaa attcaacctg accgctattc gcgatgcaga atccattcgt
     3061 acaaaacatt ttctcgattc gttctcttgt attcttgctt ggactacatc gccaccagac
     3121 catctcgttg atgtgggtac cggtgcgggt ttcccgggac ttcccctaaa aatcctttat
     3181 ccagatctga aattaacgct tgttgaatct gttggcaaga aagcaaaatt ctgccaacat
     3241 gttgtagaaa aactcggtct ggatgatgtg aaagtgatcc aaacgcgcgc agaagaaatc
     3301 ggacaggact caatacatcg cgaacaatat gattggtcag tcgcgcgggc ggttgcaaat
     3361 ctcacggtgc tgagtgaata tcttttaccg ctggtcaaag tcgggggggc aatgctcgcg
     3421 caaaaaggcg aaagcggacc tgccgaggca cagtccgctg aacaggcaat cgctttattg
     3481 ggtggaaaat tgaaacaact gatcccggtc aagttgccgg gtgtaacaga agagcgttac
     3541 cttatcgttg tagagaaaat tgatgcgacg ccatctaagt atccgcgcaa gccgggtatc
     3601 cccgcaaaac agccgttgta agtttggatt atcacttaat tccctgttca aataaccatt
     3661 tgttcagttt ggtaatcgaa tcgtttttga acctgtctgc ttcggctata tgaccttcaa
     3721 cactgaaatc ccacaaagga agctcatatt caccgattac atcgtttttt acagtagttg
     3781 tttttctgga gcttgcaaca ttcaaataat aaatggaatc acgcacatcc aaccttagct
     3841 ttacagagct ttcacctcgc gaataattaa cataccttcc caaacctcct gaggggccca
     3901 cgtcctcttt ataaccaaga gggattagcg tattctgcag aacagcttgc atttcttcga
     3961 agaattttgg gtgctttttg agagccgcgg cttccccgct tattatgctg cctatccaaa
     4021 ttgatattac aaaaatagct ataaacaaga aaatgaaccc aatgacgaga ataacccgat
     4081 atgtttttat gaaggtaagt atggcatcca ctctgcctgt ggtgtccgtt aatagtattg
     4141 tactgacaac cactataaga ataacagaaa cgatagtgcg aattttttcc ttcatctttg
     4201 tctcctcctg aaggtgaccc tgctcaaagt ttacatagaa ttattagcat gcctcatcca
     4261 caattgtgat cgtatcttcc ttatttggaa caatacacaa aaactcaaac ggctcctcgc
     4321 ctgtattttg ataggaatgg atcgcgcctt cgggaatgaa aacaacatcc cctgttttaa
     4381 cgtgatgagt ttcgtcatca attgttatcg ttgcttcccc gcgcaacaca tattgttcat
     4441 gttccaattt atttgtgtgg cgcggcatcc cgcctccctt ttgcatggaa aattttcgca
     4501 gtgcaaagtt gggaccttcc tgggcggaga tgaggacctg aatcgttgtg tccttcccgg
     4561 cagcaacatt ttttgcttct acatcacttg aatgtctgac ggacatagct taactccatt
     4621 tacatcatgg tattcccgtg tttgtgggcc agggcgtata ggttggggca atagggagtg
     4681 gcagggttgt catcgtaggt aagggagcag gcagggtagg ctgaaccaac ggcgtctgtg
     4741 tgaagagcag cggaagtgta ggcgtttgcg taggcgtcaa gttcgcattt cgcctcagca
     4801 ggctaacggt caggaacgag ccgaccacca ggacaaacat cacacccgtc acacctccaa
     4861 tcacccatgg aagccagccc atattcttcc ccactgacag tttgaaatta gatggcaccg
     4921 aaaccagaat gactgtcgtg ttatcgtgcc caccgcgtga atttgcaagt tccaccaacg
     4981 cccgtgaagc ttctttgaga ctgggcttgg agcgaacaat ttcgaggatc tcatcatccc
     5041 ataccaaatc ggtcagacca tccgtacata gaagcacaac atcattcggc agcaactgaa
     5101 tcccctggtt gttctccaga tgcacatcat catccccgtc aaaccatcgc aagcggaaat
     5161 ctggcacagg aggttcggat gatcccagat aacgccggat gacatgcaca tttggatgcg
     5221 tacgcgcttg ttcgggcgtg agaatgcctt tctccatggc ttcctgcacc caggtatgat
     5281 caatagtcaa ttgttgaata cggccagcgc gcatcaaata gatgcgtgag tctcccacat
     5341 acgccgtatg gagttgatcg ccaacaatcc aggcagtggc acaggtggcg cccatgcctc
     5401 ttaattcttc attcgaggcc gcatgggcag ccaccgcatc actggcttcc gttactgcat
     5461 cttccatggt ttggcgtatg tgtcgtgtat cactttttgc aaccgtctgc atgatgtgat
     5521 tgaccgccag ttccgccgcg acctcgcctc cacgatgacc accgattcca tcggccaaca
     5581 ctgcgaacag caccggcatt ttattgtgat catccacttg aaacgaagcc accgcataac
     5641 ggtcttcgtt gttcttgccc gtcatgcctg ggtgcgaaag cgcctcaacg ttcaaatggg
     5701 aacgcactgt acgaatcata attaaacttg tttctttgta atgacctttg gttcggacgc
     5761 cgctggaggt cgactcagat caaagcgata aaccaactgc ccaaagtgga tgcgatctcc
     5821 atgggtcagt cgacgcccat cacgcatgac gggttcataa ttcacccacg tccctgccac
     5881 agaaccatga tcataaataa taaaagttcc gtcatcaatt tgccggatgc gggcatgtaa
     5941 gggagaaatg gacgcatcgt cgagcacgcg cttggactgc accgggtctg tgccaaacgt
     6001 catatctttt tcctgaaccg gaattggtga gacactcgct ggctcaccgc catttgtcaa
     6061 acggatgaga tacgccggag cttccgcgac ccgcgcaggt tttggttgaa gccaacctga
     6121 ccgcagggaa cgtggctccg ttttcgtttt ctttgtagcc gaaataggcg gctccgtcaa
     6181 ggcgacaacc ggttgtgtaa gcgggtcttc aaactgcttg cgggtttcgc gtcgacttct
     6241 gggcgttgca gtgccagccc ggcctcgcaa cagaataccc aatagcgcaa gcccagccag
     6301 tgcaatcgca ccgatgacca agtatgaacg ataacgagca aggaatgcac gaatcccgcg
     6361 agggggctga acaatggtca acgtgaccgg aatcccaatg ctcgacttgc tgattccaag
     6421 tacatcctca gcttccacaa tgatctcatg ctgtccactt ttgctatatg cacttagatc
     6481 ccacatgaaa ctttcaaagg gtgcggaaat attttccgtt acagcctgac catccacata
     6541 taacgtggtg cgcttcaatt cccgctcatg cccgtctgga aattcgacga tgatctccac
     6601 ggcttgttgc gctgggacca atgcgtcaga gttatatgga tcatcttcag ccggctgacg
     6661 ggtaatctgg agtggcgggg atacaaagat cgggttgggt ggttgaatat ccacgctgaa
     6721 gggtttatcc agcgcgggga ttcttccctc gggtgcttca acgtacatgc caagcgtgtg
     6781 gtcgcctgcg gttttgaggg cagaggtata cgtcaggtta tagacatggc gtaacggcga
     6841 aaagaattga ttcgggtcag ggaaaggttc aacaccagaa aatgcaaaaa aggatccatt
     6901 tgtctgctgg gcaagtgatt taaacgcgtt gccactaggg gtaacagaat actccggcgc
     6961 atccacaaac cagacaaaga cacgtacttt cgaatcgatg gccctttgga taagcggagc
     7021 aatcgtagaa tctatacctg catcatccat atgaggggtg atgagcagaa tagcacgctt
     7081 catacccggc tgccgcgact gcgcggtgac tgtatccagc gcaatggaga gagtttgtaa
     7141 atttggggtc gtactacgaa aatcgggctt aaacgaaccc aaactcacaa accaatcttt
     7201 ggcagttgca tgcgtgatga gagagcccga taacgagacc aggctcaagt catcttgaga
     7261 atcagcaggc tgtgcatcgg cccatctgcc tagcatatcc acaacatagg taaagcgagg
     7321 aactgcattt gcatcacgga ccgccagcgc ggggccggga ttgatcgcaa ccacaatctg
     7381 gaccggaaca tttgactctg aaatcgcatc tgcattccgc ggttcgccat cttcatagac
     7441 ggtgaaatca gaagcgcgca gaccagtcat gaactccccg ttcgcgttgt acacatctac
     7501 cagagtagag atttgaggga aattctgcgc gtcaacggat gcaatctctg catacgcggc
     7561 ggtttgcgcg cgtgcagagg tgaaaggaac cgccagcaga ctcagactta gaatgtaggt
     7621 gaggaattta ctcattcgcc tcagggagaa tcaccggctc cgcgatctgt ggctttgtca
     7681 gacgcatata catcaacgtc caggccgatt ctgtatatgc aatcgcaatg ccgctgagca
     7741 ccaacgagac tggaaaatat aaacagatac atacgccagc aatggctaaa ggcgtcatgc
     7801 tttgtgcttc gcccgccata acggcgatga aggccggtac taaaatcaca aagatcggta
     7861 gggcaaggac gaaacccgca gcgaaaccga tcactgccag aatgatcgcc ataatgatga
     7921 cgggtcccaa attattgcga aatacttccc atcctcggct tagcgcaggc aggacgccca
     7981 tctcttccag caccagggca ttttctgcct gccggaagat tacgccaaga acgagcatca
     8041 ccggcacaag cagacaaaca catccaataa gcaacgggaa agtagcaaaa aagcccagac
     8101 ccgaggcatc actgccctgg cttgcagcaa gtccgaaaat tgcgaatacc gcaatgatca
     8161 tggcgaaaat gaagaccggc agggaaagga tgagggacaa tccgagcatt cgccaaaagt
     8221 agggtgtgct ctcgctgaat agctggccaa agattaggcc ttctgcacca ccatcgattt
     8281 gggcggtacc gcgtataagt ccaactttcc caatcgtgct gagaaagatg gtaacgatcc
     8341 aaattagaca aacgatcgca acggtgccta ccaggaaagc agtcagattt tgctcaaccc
     8401 attgaaagaa ttccgtgaat tgaggtggta gatcgggcga agtccccggt ccgccattgc
     8461 cctgaaagct attgctgttg aa
//
LOCUS       DASTJP010000531         3195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1498962, whole genome shotgun sequence.
ACCESSION   DASTJP010000531 DASTJP010000000
VERSION     DASTJP010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498962"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFQ23_15575"
     CDS             <1..522
                     /locus_tag="VFQ23_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908071.1"
                     /translation="TSQMVVLNYSPPGCLRVLDSDIDFDNRLLDPLLREGAVLSNSVM
                     IRADRSVTMPESLFGLEPAHGWCYYFEKADLARQFGDWDMVVELGDTAFKLESDSPND
                     PIERFVFIEGYAHAGEWERAVELSKVSYRVSREYVGPLLCRLWKRIETETMESPERSE
                     ALTDVTNTFACNP"
     gene            699..1907
                     /locus_tag="VFQ23_15580"
     CDS             699..1907
                     /locus_tag="VFQ23_15580"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9908072.1"
                     /translation="MRKSLLQVFFVLTVLSLVLSACGGAAATTEAPPAEQFVFGMLLV
                     GPYNDHGWSQAHYEAGKYVEEKLGAKMVYLDKVNPADRPGTTPDQLAADLVAQGAKLV
                     IFNSDDMKDASTTFAKANPEIDVIMASGDQVWADGKAYEEIPNLANIMGRMEYGKMIG
                     GCAAALSTNTGKIGYLGPLINDETRRLAASAYLGAKHCWTEAGNDSADLEFKVTWIGF
                     WFNIPGVTSDPTQVANDFLNSDYDVVISGIDTTEGLTETKKAADAGKTVFAIPYDYIG
                     SCEEGASVCLGVPYFNWGPAYLNEIKAASEGNFKSSFQWNGPNWSDINNPDTSAIGFV
                     KGDALSAEDATQLDAFIAALAGGLNLWTGPINLQDGTSYLGDGEVATDQQVWYLPQLL
                     EGMEGQSVPQ"
     gene            1990..>3195
                     /locus_tag="VFQ23_15585"
     CDS             1990..>3195
                     /locus_tag="VFQ23_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9908073.1"
                     /translation="MKVELRGIHKYFGKNHANDNITLTIPSGTIQGILGENGAGKSTL
                     MKILSGFIHADEGEILLDDKPVKISSPADAIRHGIGMLHQDPLDFPSMRVLDNFMLGH
                     SGGLFPNRSAVAREFKSLAERLEFSLDPDSYVDLLTVGERQQLEILRLLWLGARVLIL
                     DEPTTGISASQKEKLFLTLINKLGVQGMTILFVSHKLEDVEILSDSIAVFRQGKLVGE
                     AKRPANTEKLVEMMFGKNITIGGRQAMGQERNVLSLNDVCIEDSRLRIQDLNVDIRAG
                     EVIGLAGLEGSGQSSFLRACAGLIRPVSGHVKLNDKDFTGKTYHTFKSNGVEFLPAAR
                     LEEGLVPGLTLTEHFVLAGKQKGVFIDRESGRNVTEERIKAFNIKGTPVSPIESLSGG
                     NQQRALLSLL"
BASE COUNT          819 a          713 c          863 g          800 t
ORIGIN      
        1 acatcacaaa tggttgtgct caattattca ccgccagggt gtttaagagt acttgattcg
       61 gatattgatt ttgataacag attgctcgat ccactcttgc gcgaaggagc tgtattgagt
      121 aattcagtca tgatccgtgc agatcgctcg gtgaccatgc ctgaatcact ttttggtttg
      181 gagcccgcac acggttggtg ctattatttt gagaaggctg atctcgcgcg tcagtttggt
      241 gattgggata tggttgttga gttaggcgat acggctttta aacttgaaag cgattcgccg
      301 aatgatccga tcgaaaggtt tgtgtttatc gaaggctatg ctcatgcggg ggaatgggaa
      361 cgcgcggtag aattgtcgaa agtgtcgtat cgagtgtcga gggagtatgt cgggccatta
      421 ctgtgtcggc tatggaaacg tatcgaaacg gaaacaatgg aaagcccaga acgaagtgaa
      481 gcactgacgg atgttacgaa cacatttgca tgtaatccat aaaagtactt gctaccctga
      541 tttgatttgc ctacaataat gccacgtttc agagataatg tataattgta tagacaatat
      601 gtcgaactct agctggtgaa aggaggcggt cgtcaacctg aattagcttg ctacgcgtag
      661 gatctgatgt tctaaataaa caaaagagga gagtagaaat gcgtaagtca cttttgcaag
      721 ttttttttgt actgacagtt ctttcgctgg ttctgtcagc ctgtggtgga gccgccgcga
      781 caaccgaggc gccgcccgcc gaacagttcg tatttggtat gcttctcgtt ggtccataca
      841 atgaccatgg atggagccag gcgcattatg aagctggcaa gtatgtcgag gagaaactcg
      901 gcgccaagat ggtctatctg gataaagtga atccggccga ccgtccgggc actactccgg
      961 accaactggc tgctgatctc gtggcgcaag gcgcgaaatt ggttatcttc aattcagatg
     1021 acatgaagga tgcttccact acctttgcca aagccaaccc ggagatcgac gtgatcatgg
     1081 cctctggaga tcaggtatgg gcggatggaa aagcctatga agagattccg aatctggcga
     1141 atatcatggg acgcatggaa tatggaaaga tgatcggtgg ttgtgccgca gctctttcga
     1201 ccaataccgg aaagatcggc tatctcggac cactaatcaa tgatgagact cgccgtcttg
     1261 ccgcctcagc ttacctcggc gcaaaacact gctggaccga agccggaaac gactctgccg
     1321 acctggagtt taaagtaacc tggatcggat tctggttcaa catccctggc gttacctcgg
     1381 acccgaccca ggtcgccaac gattttctca acagcgatta tgatgtcgtg atttctggta
     1441 ttgacaccac agaaggactc actgaaacca aaaaagccgc tgacgctggc aaaaccgtct
     1501 ttgcaattcc ttacgactac attggttcct gtgaagaagg tgcgagtgtt tgtttgggcg
     1561 tgccatactt caattggggt cctgcatacc ttaacgagat caaggctgct tccgaaggaa
     1621 acttcaaatc aagcttccag tggaacggac cgaactggag tgacatcaat aaccccgata
     1681 cgagcgcgat tggattcgtg aagggtgatg ctctcagcgc agaggatgcc acccagcttg
     1741 atgcatttat tgccgcattg gcgggcggtc ttaatctgtg gaccggtccg attaacctgc
     1801 aggacggtac gtcctatctg ggagatggag aagtggcaac agatcagcaa gtctggtacc
     1861 tgccgcaatt actcgaaggc atggaaggcc agagcgttcc tcaataatct gctcaggatg
     1921 tgggaaggcg atttctgcct tcccacattt tttattcacc ttcaaggaag tctctatagc
     1981 gtaaagccta tgaaagttga actgcgcggc atccacaaat acttcggcaa aaatcacgcc
     2041 aacgataaca tcacactcac gatcccttcc ggcaccattc agggaatcct gggcgaaaac
     2101 ggcgcgggca aaagtacgct gatgaaaatt ctctccggct ttatccatgc cgatgaggga
     2161 gagattttgt tggatgacaa gccagttaag atttcatcgc ctgcagatgc gattcgacat
     2221 gggattggca tgctgcatca ggacccactt gattttcctt ccatgcgcgt gcttgataac
     2281 ttcatgcttg ggcattctgg tgggctattt cccaatcgtt ccgcggtcgc tcgggaattt
     2341 aagagcctcg ccgaacgact cgaattctcg cttgaccctg actcctatgt ggatttgctt
     2401 acggtagggg agaggcagca gttggaaatt ttacgcctgc tgtggctggg tgcgcgggta
     2461 ttaatcctgg atgaaccaac aacaggcatt agcgcttcgc agaaagaaaa actttttctc
     2521 acgctaatta ataagttagg ggtccagggt atgaccatcc tctttgtctc gcataagctg
     2581 gaagacgtgg aaatattaag tgattcaatc gctgtgtttc gtcagggaaa acttgtaggg
     2641 gaagccaaaa ggcccgcgaa tacggaaaaa cttgtggaga tgatgtttgg caagaatata
     2701 acgattggcg ggcgtcaggc aatgggtcag gaaagaaacg tattgagttt gaatgatgta
     2761 tgcattgaag atagccgtct tcgcatccag gatctgaatg ttgacattcg ggcgggcgag
     2821 gtgatcggac tggctggtct cgaaggtagt ggacagagtt cattcctgcg ggcctgtgcc
     2881 gggttgatca gacccgtatc tggacacgtt aaattgaacg acaaagattt caccggcaag
     2941 acgtatcaca cgttcaaaag caacggagtg gaattcctgc ccgctgcgcg cttggaggaa
     3001 gggcttgtgc caggcctgac actcactgag catttcgtat tggcgggaaa acaaaagggt
     3061 gtgttcattg accgtgaaag tggacgcaat gtgaccgaag agaggataaa ggcttttaat
     3121 atcaaaggca cacctgtcag tccgattgaa tcgttgtccg gcggaaacca gcaacgcgcc
     3181 ctgctttcat tgttg
//
LOCUS       DASTJP010000532         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_138261, whole genome shotgun sequence.
ACCESSION   DASTJP010000532 DASTJP010000000
VERSION     DASTJP010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138261"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..619
                     /locus_tag="VFQ23_15590"
     CDS             437..619
                     /locus_tag="VFQ23_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908074.1"
                     /translation="METRNASAGARMYEILMRGSSTATCLHCLIATFERFPIASRNLL
                     IERAIEHAAYGTINHR"
     gene            complement(1275..2306)
                     /locus_tag="VFQ23_15595"
     CDS             complement(1275..2306)
                     /locus_tag="VFQ23_15595"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9908075.1"
                     /translation="MNPSIPSTMQAVQLDEPHGRLTLREVRVPRPGAGQVLIRMTASP
                     INPSDLGSLSGFSYSGTRQFPFTPGLEGSGTVVEAGEGFMPRLLNGRRVACTPSLMGD
                     GTWAEYMVTSAKSCVPLNRNVSLQQGAMMLVNPLSALAFFEIAERGKHRAIVNTAATS
                     ALGGMILRMGKRRGIPIIHVVRRQEQVDLIREQGGEYVFNSSEADFPEQLRTMVHRLQ
                     ATLLLDAIGGSMTKALGQAAPYGSTILLYARLSAEDSLLDARAALAKQLHFDGWFLGN
                     WMREKNLIQTLRLARQAQSLLGTDLSTPVQKRLPLSAAQQGLEMYTRNMGAGKILLVA
                     NPQEVNLEG"
     gene            2402..3085
                     /locus_tag="VFQ23_15600"
     CDS             2402..3085
                     /locus_tag="VFQ23_15600"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9908076.1"
                     /translation="MSWKTVSYDPQKHHRRSIRLKGYDYTQAGAYFVTICAYQRMHVF
                     GEVINGEMIPNDTGKTARQEWFKTAELRPYVKLYDDEFVIMPNHGHGIIWIVDDNVGI
                     RRRRIQDLEKQGAQQRRAPTEKTESFGRPVKGSIPTIVRAYKSAVTYAANTADKTRGS
                     VLWQRNYYERIIRNDRQLNNIRWYIRNNPLHWQLDRDNAQNVRKLSPPANVDEYLKDV
                     DEMLKIKRR"
     gene            complement(3224..>4017)
                     /locus_tag="VFQ23_15605"
     CDS             complement(3224..>4017)
                     /locus_tag="VFQ23_15605"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HET9908077.1"
                     /translation="GSLMIIHSTLANNAASSAGGGVFNQGTLTLKNSIVADSAAGGNC
                     SGTTTNGGGNLSYPDATCPGVNGDPRLAALANNGGSTQTMALQAGSAAINAINMVNGS
                     CNDTGVTSDQRGVTRPQGAQCDIGAFELEQAAPTATSTNTYTPTNAPTNTPTYTFTPV
                     PTNTPTYTFTPLPTDTPVNTATATAMPSPTGTMSQTQTSLPTSTSTNTLTAMPTSTFT
                     NAPVNTAKPTNTLRPTNTPRPTNTVRPTNTPKPTNTPRPTHTPRR"
BASE COUNT          982 a         1008 c         1021 g         1006 t
ORIGIN      
        1 tctttggtta caaagaaaat aatgtatttc gtacccactg gagtaaccac gccttttgag
       61 atcgcatgga aactttgata accccaaagt ctcgctaaat aaggacgttc gtagctttga
      121 ccaaatttga tagcattgaa actgatttgc atagaaaatt ttcaaaggaa tattacacgt
      181 aaaaaaagtt ttctaatatt aagtataact cgactatcct tattaagaac agtataacct
      241 agtgatggaa ataactgcac aaaggctgaa gcgcaaccgt ctgcgactaa tacaaaacgt
      301 aagacattat cccttcgtcg ggtcttggct ttctacattc atggtcaaag atagctgctt
      361 acttcctact ttgtggatta tctaaatcag ctgggcttaa tgcgccctgc ctctaactct
      421 cgcgacctga gcgtcaatgg aaactcggaa cgcgtcagcg ggtgccagga tgtatgaaat
      481 cttgatgcgg ggatcatcca ccgccacctg cctgcattgt cttatagcaa cgttcgagcg
      541 ttttccaatt gcgagtcgta accttcttat tgaacgcgcg atcgagcacg ctgcatacgg
      601 caccatcaat catcgctagc actttatatc ccttctgcgg cgcgtcgtaa ggtatgtcaa
      661 atggcaacct gccgtacccg gcaggttttg ttattgaccc ccaccccggc ctcccccatt
      721 cgacgaacac gaatggggga ggagaataaa atatccttat gaacattcaa attcatattc
      781 accctctgat tacccctccc ccaattgtga atttcaattg ggggaggttc gggagggggt
      841 cgaatgagca acgtccattt tcgaaaccag cacgccatcg gtacctacat cgtcgatttt
      901 ccctggcacc gaacgccagg acgatgtgcg caccgcgaag aaaactaatc attgaattag
      961 acggaagtca acatttggaa cagaaggaat atgattaaga aagaactaag tatttggaag
     1021 cgcgtggtta tcgaattctg aggttttgga acaatgatgt catgaacaac actgagtctg
     1081 tgctaaaggt tatctggaat atgctcaatg aggaaaagca gtagatgatc tacctaaacc
     1141 aaaagcccgc cttatcactt aggcggggtt ctttttattt ttgaatgtca ttttgaaata
     1201 tctataagct attcgtttaa cctccctaat catttactga cagcgtactt ggaccctgct
     1261 tcggcgaggt cctttcaacc ttcgagattc acttcctgcg gatttgccac caataggatt
     1321 ttccctgcgc ccatattgcg ggtgtacatt tcgagaccct gctgtgccgc cgagagcgga
     1381 aggcgtttct gaactggagt acttaaatca gtccctaaca gagattgtgc ctgccgggct
     1441 aatcgcagcg tctgaatgag attcttttcg cgcatccaat tgccgaggaa ccagccgtca
     1501 aaatgcagtt gcttcgccag ggcggcacgc gcatccagga ggctgtcttc agcggacaaa
     1561 cgtgcataca gcaggatcgt gctgccgtat ggcgcggctt ggccgagagc cttcgtcata
     1621 ctgccgccga tggcatccag caaaagagtg gcctgtaacc tatgcaccat cgttcgtaat
     1681 tgctcgggga aatccgcctc actgctgttg aaaacgtatt caccaccctg ctcgcggatc
     1741 agatccacct gctcctgccg ccgcaccaca tgaatgatcg gaatgccccg gcgtttcccc
     1801 atccgcagga tcatgcctcc cagggcgctt gtggcagcgg tgttgacgat tgcgcgatgt
     1861 ttgccgcgtt ctgcaatttc aaagaacgcc agtgcgctca acggattcac cagcatcatc
     1921 gcaccctgct gcagactcac gttcctgttc aagggtacac aggactttgc cgaggtgacc
     1981 atatactctg cccaggttcc atcgcccatc agtgatggag tgcaggcaac gcgccgtccg
     2041 ttcaacaggc gcggcatgaa cccttcccct gcttcaacga cggtcccgct gccttccaac
     2101 ccgggcgtga aaggaaattg acgtgtgcca ctgtaactga agcccgatag cgacccgagg
     2161 tccgaggggt tgatcggaga ggccgtcata cggatgagga cctgtcccgc ccccggacgc
     2221 ggcacgcgga cctcgcgcaa ggttaatctt ccgtgtggct cgtccaattg aaccgcttgc
     2281 atggtggatg gaatggatgg attcatttat atctccaaaa actattctac cgcttgtcag
     2341 atgatgttac ctgtgtgata cgattaacca tgtacaaaag atatagcaaa accttgtcca
     2401 tatgagttgg aaaaccgtga gctacgatcc acaaaaacac catcgtcgat ccattcgcct
     2461 caaaggatac gattacaccc aggcgggcgc gtatttcgtt acgatctgcg cataccagcg
     2521 tatgcatgta ttcggtgaag ttataaatgg cgaaatgatt ccgaatgaca ctggcaaaac
     2581 tgcgcggcag gaatggttca agacagcaga attgcgtccg tatgtaaagt tgtacgatga
     2641 tgaatttgtg atcatgccca atcatgggca tgggatcatt tggattgtgg atgataacgt
     2701 agggatacgg cgacgccgta tccaggattt ggagaaacag ggggcacagc aacgccgtgc
     2761 ccctacggaa aaaaccgaat catttggtag gccggtgaaa gggtccatac caacgattgt
     2821 ccgtgcgtat aaatctgcgg tcacctatgc agctaatacc gcagacaaga cgcgtggttc
     2881 tgtcctgtgg caacgcaatt attacgaacg catcatccgc aacgaccgcc aattgaataa
     2941 cattcgatgg tatatccgca acaatcccct ccactggcaa ttggatcgcg acaatgccca
     3001 aaacgttcga aaattatctc cgcccgcaaa cgtggatgaa tatttgaagg atgttgacga
     3061 gatgctcaag atcaaaagga gatgacctgt ttgccagatg aaattgctcc gctgctatcg
     3121 aacaaaagag actcgccgta gggcgagtct cttttcgttt gcgatcaaat gggaatgacg
     3181 ttttgaactt atacatctca tcaccacccg cttgcaccgc cattcacctc ctcggtgtgt
     3241 gtgttggcct aggcgtgttg gtcggcttgg gtgtgttagt tggtctgaca gtgttcgtgg
     3301 ggcgcggtgt attggttggt cggagtgtgt tcgtcggttt cgctgtattg accggcgcgt
     3361 tggtaaatgt actcgtcggc attgcggtca gcgtattcgt ggaggtactg gtcggcaggc
     3421 ttgtctgtgt ttgggacatc gtccccgtag gggaaggcat cgcggtagcc gttgccgtat
     3481 tgacaggtgt gtctgtcgga agaggtgtga aggtataggt gggtgtattt gttggaacag
     3541 gtgtgaatgt atacgtcggt gtatttgttg gggcatttgt gggcgtatat gtattagtgg
     3601 atgtagctgt cggcgcagct tgctcaagct cgaacgcacc gatgtcacat tgcgcgcctt
     3661 gcggacgcgt cacgccgcgc tggtctgagg tgacaccggt atcattacag cttccattga
     3721 ccatattgat ggcattgatc gctgcactgc ctgcctgcaa agccatcgtt tgcgtggagc
     3781 ctccgttatt ggcaagcgcg gcaagccgtg ggtcgccatt cacgcccggg caggtggcat
     3841 ccgggtagct caaattccca ccaccattgg tcgtggtacc cgaacaatta ccgcccgcag
     3901 cagaatcagc aacaattgag ttcttcaagg tcagtgtgcc ctggttgaaa acaccaccac
     3961 ctgcactact ggcagcatta ttcgcgagcg tgctgtgaat gatcatcagc gatccga
//
LOCUS       DASTJP010000533         6362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_802455, whole genome shotgun sequence.
ACCESSION   DASTJP010000533 DASTJP010000000
VERSION     DASTJP010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802455"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..581
                     /gene="nusB"
                     /locus_tag="VFQ23_15610"
     CDS             150..581
                     /gene="nusB"
                     /locus_tag="VFQ23_15610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET9908078.1"
                     /translation="MKPRTRARSLALQVLYEVDIADHPPAEVFKSRLDDSSLSNELAD
                     FARQIIFGVLPLTTSLDHLIAKYAPEWPLDQIAAIDRNILRMALWEFAVLRETPIKVA
                     INEAVELAKLFGSDSAPRFVNGVLGALADHQHEIQQAISKA"
     gene            594..1001
                     /locus_tag="VFQ23_15615"
     CDS             594..1001
                     /locus_tag="VFQ23_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908079.1"
                     /translation="MDILGVGWQELIFIFLIALIVLGPRDMQKAGKTIGRWLNQLVRS
                     DGWKALQQTSRELRKLPNTLMKEANLEEFQETGQAIRQGLDLNPKRPASPATDRRRTP
                     PNEPENTIRPPSVNPQSNAPDAEDGAGSAKENG"
     gene            994..1926
                     /gene="tatC"
                     /locus_tag="VFQ23_15620"
     CDS             994..1926
                     /gene="tatC"
                     /locus_tag="VFQ23_15620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9908080.1"
                     /translation="MANLLRALWRVITFPFVLIFNILAFPIRALQRIFRFLNTEPEDR
                     PVMEAFSSLAAEQEARQSLWDHVEVLRAHLLRIVIAIAVGVTISFYFAVPLMGYLATP
                     IGGLENLQAIEVTEELSVFMRVALTSGIALMLPYIAFELWLFAAPGLRARERKFSLIG
                     IPFATLLFLGGMAFTFYVLLPTALPFLGGFTNIAEFWTAREYFSFITGLMFWIGLFFE
                     FPLVIYILSSIGFVEPGILVSQWRLAIVLISIIAAVITPTIDPITMGLVMLPMTLLYF
                     VSIGLSYIAYAGRRRRVMSEMLETDLQDQSKVHL"
     gene            1951..4458
                     /locus_tag="VFQ23_15625"
     CDS             1951..4458
                     /locus_tag="VFQ23_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908081.1"
                     /translation="MKPKSIFPRQILLFIVILALASQACSISLLEWPIFPTPSGPGNP
                     TPGGPTPTPPPRAQMTFTVRLPEPLPANEILALSVLDEVTGLSLNAVDYQLTAVDTIT
                     YSATLAIPDKAIIKYRYLRRGATHIVEDTNTDGAIRYRLVHVNGPTQIVDTVNSWSDK
                     PVNTISGNIYGTVLNASNGVPIPDIMVTAGGLQTQTDSAGRFQLIGLRGGAHNLVAYA
                     LDGMFQTFQQGANVAENQGTPVEIRMNPAQLVNVIFTVSVPQNTQRGVPVRLAGNLLQ
                     LGNTFSDLRGGLSTVADRMPVLSPLPDGRYSVSLFLPAGADVEYKYTLGDGFWNSEFT
                     SDGRYLTRHLIVPQQTISIDDTVQSWQAGPNAPIVFEVAVPADTPSGDVVYIQFNPYG
                     WTPPIPMWNTGPNRWSYKLFGPLNIVGSFGYRYCRNAQCDSADDSQTTGPNTRGRTVA
                     PSIAPQDIVDTVNQWVWNQKAGSAALVATNIQSRGAGFMAGVEFQSYYDPNVPAFNAR
                     ALQNVQALGSNWVIYSPTWTYSSISPLTFTEQPGKDAFWADTTNAVAQARAINLNVAL
                     FPQPRFATDANEFWKNAPRDGNWWSAWFDHYRAFAVHHADMATLSGAQALIIGGDWIA
                     PALPGGTLADGTSSGAPVDAEARWKTVIAEVRQHFRGNLLFAIPYDNSVLQPPINILR
                     DADAVYLLWFAKLSDQANPNKADMTAEAGRLLDNNVAPVQIQVNKPFIIALSYPSSTS
                     SGTGCIPNGNGGCLDWKMLSRPNADVNTVNLDMQQQVDIYDAMFNAVNTRSWVSGFVS
                     RGFHSPVVLLDKSASVYGKPAADLLWYWFPRLLGNIK"
     gene            4671..>6362
                     /locus_tag="VFQ23_15630"
     CDS             4671..>6362
                     /locus_tag="VFQ23_15630"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET9908082.1"
                     /translation="MLKTILKRVGLGLLIIVLLAGTGGAYYFKSYLPNTVAPKSFPQV
                     DGEIQLEGLDGAVDIYRDNMGIPHIYATTTHDLFFAQGFVHAQDRFWQMDFWRHVGSG
                     RVAEMFPSQVETDAFIRTLGWRVTAEQEWEQLPPPLKDQLNAYTEGVNAYLKDHEATA
                     LSLEYTILGLLNANYKIEPWTPVHSLTWGKAMAWDLRGNMGAEIERAALLKTLTPQQV
                     EALFPPYPQDLPVIVNKIGNGDSADLQTQSIAFDIPEETLAALQHNTSLLDVALGPVG
                     DGIGSNSWAISGSRTSTGLPLLANDPHLSIQMPSIWYQAHLECKPVSEACPYNVAGFT
                     FAGVPGVIIGHNQRIAWGLTNVGPDVMDLYIERVNPDNPNQYEVNGQWVDFETRKEVI
                     HVAGGDPVEITVRSTRHGPVISETYGPLKNENTNDDPKFIPFKERVGIDLPEQYVISL
                     KWTALSPSTPFEAVWGFNLAQNWKEFREAAQHFHVPAQNLLYADVEGNIGYQMPGDIP
                     IRANGEGTFPVPGWTDEYEWTDFIPFEELPFTYNPPEGYIVTANNQVPPRDYPYLI"
BASE COUNT         1505 a         1654 c         1611 g         1592 t
ORIGIN      
        1 ctgaaaaaag acatgaatat tcgcgaggtg agtcgtaacg tgcagatgca ggtggcacgc
       61 gccttgcagg aaatggtcgg tatggatatt gggaaagtcg aaatccacat tgaagatatc
      121 gattatgagg aactagaggc agagtcggga tgaaaccacg caccagggcg cgttccctcg
      181 ccctgcaagt actttacgaa gtggatattg cagatcatcc tccagcagag gtattcaagt
      241 ccaggctgga tgactcctct ctctcaaacg aactggctga ctttgcccgt cagatcattt
      301 tcggtgtact tcccttaacc accagtcttg atcatcttat cgccaaatac gcccctgaat
      361 ggccgctcga ccagatcgcc gcgatcgacc gcaatatatt gcgaatggca ctttgggaat
      421 ttgctgtgct tcgagagact cccataaaag ttgccatcaa cgaagcggtg gaactggcga
      481 aattatttgg ctccgattct gcaccgcgtt ttgtgaacgg cgtgcttggc gcgctggctg
      541 atcaccagca tgaaatccaa caggcaatct caaaggcata atgtaaattc gatatggata
      601 ttcttggcgt tggctggcag gaattaattt ttatatttct tattgctttg atcgttctcg
      661 gtcccaggga tatgcaaaaa gctggcaaga ccatcgggcg ctggttgaat caactggtcc
      721 gatcagacgg ctggaaggca cttcagcaaa cctcgcgcga gcttcgtaaa ttgccaaaca
      781 ctttgatgaa ggaagcgaac ctggaggaat ttcaggaaac aggacaagcc attcggcaag
      841 gcctggactt aaacccaaaa cgcccagcat ctcctgccac cgacaggagg cggactccgc
      901 caaacgagcc ggagaacacc attcgacctc cttccgtcaa tccgcaatcc aatgctccag
      961 acgccgagga tggcgctgga agcgcaaaag aaaatggcta atctcctacg agcactgtgg
     1021 cgggtcatta catttccatt tgtgctcatc ttcaacatcc tggcttttcc aatacgtgcg
     1081 cttcaacgca tttttcgttt tctgaatacc gagcctgagg atcgcccggt gatggaggca
     1141 ttctcaagtc tcgcggcaga acaggaagcg cgacagagtc tctgggatca tgtggaagta
     1201 ttgcgtgcgc atctcctgcg cattgtgatc gcgattgcag tgggcgtgac tatttcattc
     1261 tatttcgcgg tgccgttgat gggatacctc gcgacaccga tcggcggctt ggaaaattta
     1321 caggcgattg aagtcacgga agaactgagt gtattcatgc gtgttgcgtt aaccagcggc
     1381 attgctctga tgctccccta tattgccttc gaactctggc tgtttgcggc gccgggctta
     1441 cgagcgcgtg aacgtaagtt cagcctgatc ggtattccat ttgcaacatt attattcctt
     1501 gggggcatgg cattcacatt ctatgttctc ctccctaccg cacttccatt tttaggtggc
     1561 tttacaaata tcgctgaatt ctggacagca cgcgagtatt tcagtttcat cactgggttg
     1621 atgttctgga tcggtctgtt ctttgaattc ccattggtga tttacattct tagttctatc
     1681 ggatttgtgg agccagggat attggtaagc cagtggagac tcgcgatcgt cctgatttcc
     1741 atcatcgccg cggtcattac accaacgatt gatcctatca cgatggggct ggtcatgctt
     1801 cccatgacac ttttatattt cgtcagtatt gggttgagtt atattgccta cgccgggcga
     1861 cgccgaagag tcatgtctga gatgctggaa acggatctcc aggatcaatc aaaagtccac
     1921 ttataaggtt ttctttggag aggagagaga atgaaaccaa aatcaatttt tccgcgtcaa
     1981 attcttttat ttatagtgat ccttgcacta gctagtcagg cttgttcgat ttcgcttctt
     2041 gaatggccga tctttccgac gccctccggc cccggaaacc caactccagg cggaccaacc
     2101 cccacacctc cgccgcgcgc ccaaatgaca tttacagtac gattgcctga gcccctcccg
     2161 gcgaatgaaa tccttgctct cagcgtactc gatgaggtca ccgggctttc actcaacgcc
     2221 gtggactatc agcttaccgc ggtcgatacg attacttatt cagctacgct cgccattcca
     2281 gataaagcca tcatcaaata tcgctatctg aggcgcggcg cgacacatat cgtcgaggac
     2341 accaacacgg atggcgcaat tcgctatcgc ctggtgcacg tcaatggacc aacacaaatt
     2401 gtggatacgg tgaacagttg gtctgataaa cccgtaaata caatctcagg caatatttat
     2461 gggacggtat tgaacgcatc taatggagtg ccgatcccgg atattatggt gactgccggt
     2521 ggactccaaa cgcaaacgga ttcggctggg cgatttcaat tgatcggctt acgcggtgga
     2581 gcacataatc tcgtggcgta tgcgctcgat ggaatgtttc agaccttcca gcaaggcgcg
     2641 aatgtagctg agaatcaggg tacgccggtt gagatccgca tgaacccggc gcaattggtc
     2701 aacgtgattt tcacagtctc cgtaccgcaa aacactcagc gcggcgtgcc cgtgcgactc
     2761 gctggaaatc ttctccagtt gggaaatacc ttctccgatt tgcgcggcgg cttgagcacg
     2821 gtcgctgacc gcatgccagt actatcaccg ttgcctgacg gtcgttattc cgtttcgctg
     2881 ttcctccctg ccggtgcaga tgtggaatac aagtacacgc tcggtgatgg gttctggaat
     2941 tcagagttta catcagatgg cagatattta acgcgacatc tgatcgttcc gcaacagacc
     3001 atatcaattg atgacaccgt ccagtcctgg caggcaggac ccaacgctcc gattgtgttt
     3061 gaggtggcgg tgcctgcaga cacacccagt ggcgatgtcg tttacattca attcaatcca
     3121 tacggctgga cgccgcccat tccaatgtgg aatactggtc ccaatcgatg gtcgtataaa
     3181 cttttcggtc cactgaatat cgtcggttcg tttggttatc gttattgccg caacgcgcaa
     3241 tgcgactcag cagatgactc tcaaacgaca ggcccgaata cacgtggacg aaccgttgca
     3301 cccagtattg caccgcagga tattgtggat accgttaacc agtgggtctg gaatcaaaaa
     3361 gctgggagtg ctgcattggt cgcaaccaat atccaatcgc gcggtgcagg ctttatggct
     3421 ggcgttgagt tccaatcata ctacgacccg aatgtgcctg ccttcaacgc tcgtgcgttg
     3481 cagaacgtcc aggcgcttgg ctcgaattgg gtgatctatt ctcccacatg gacgtatagc
     3541 agtatcagtc cattgacttt cactgaacag cctggcaagg atgccttctg ggcagatact
     3601 accaatgcag tggcgcaggc acgcgccatt aatctgaatg tcgccttgtt ccctcagccg
     3661 cgctttgcca cggatgccaa cgagttctgg aagaacgcac cgcgggatgg caactggtgg
     3721 agtgcctggt tcgatcacta ccgcgccttt gccgtccatc acgcggacat ggcaactctt
     3781 tccggcgcgc aagccctgat catcggcggc gattggattg cacccgcgct tccgggcggc
     3841 acgcttgccg acggcacatc aagtggtgct cccgttgatg cagaggcgcg ctggaagacg
     3901 gtgattgcag aggtccgcca gcatttccgc ggcaacctgc tttttgctat tccttacgat
     3961 aatagtgtgc ttcagccacc catcaacata cttcgagatg cagatgctgt atatctgctg
     4021 tggtttgcaa aactcagcga tcaggccaat ccgaacaaag cagatatgac cgctgaagcc
     4081 ggtcgcctgc ttgataacaa cgtcgcgcca gtgcaaatcc aggtcaacaa gccttttatc
     4141 atcgcgcttt cctatccatc ttcgacatct tccggaactg gctgtattcc caatgggaat
     4201 ggaggctgcc tggattggaa aatgctcagc cgccccaatg ctgatgtcaa cactgtgaat
     4261 ttggatatgc agcaacaagt tgatatttac gacgcgatgt tcaacgcagt taacacgcgt
     4321 tcctgggtca gcggttttgt cagccgcggt ttccattcac cagtggtatt gctcgataaa
     4381 tctgcatcgg tttatggaaa gccggccgcg gatctactgt ggtactggtt cccaagattg
     4441 cttgggaata tcaagtaatt atgaaaaagg tgacagtcac gtttgaatgt gactgtcacc
     4501 tgcaccgctc cttgcaaaaa tcaataacgc tgtgtgagcc tcaaacctgg ttatatagca
     4561 ggttttcttg cagaagaggt cagttgatat tcgtcttcaa attacatcat gttatacact
     4621 gagatctttt agaagttttt tgagcaacaa atacagttac cggaggcgtc atgttgaaaa
     4681 caattctgaa gcgcgtgggg cttggtttgt tgatcatcgt ccttttggct ggcactggcg
     4741 gagcttatta tttcaagagc tatcttccaa acaccgtcgc gcctaaatcc tttccacaag
     4801 tggatgggga aatccaactc gaaggactcg atggcgcggt agacatttat cgtgacaaca
     4861 tggggattcc tcacatttat gccaccacca ctcatgatct tttctttgcg cagggatttg
     4921 ttcatgctca ggatcgcttc tggcaaatgg acttctggcg gcacgtcggc tcaggccgag
     4981 tggctgaaat gtttccctct caggtggaaa cagatgcctt cattcgcacc ctgggctggc
     5041 gcgtcactgc tgaacaggaa tgggagcaat tgcctcctcc actgaaggat cagctaaatg
     5101 catacaccga gggtgtgaat gcctacctga aggatcacga agctacggct ttgagtctgg
     5161 aatatacaat tcttggtctg ctcaacgcta attacaagat cgagccgtgg actccagtcc
     5221 acagcctgac gtggggcaaa gcgatggcct gggatctgcg cggcaacatg ggcgcagaga
     5281 tcgagcgcgc cgcattgttg aaaacactca cacctcaaca agtggaagcc ttatttccgc
     5341 cgtacccaca ggacctgcct gtgatcgtca acaagatagg gaacggggac tctgctgacc
     5401 tgcagactca atcaatcgca ttcgacatcc ccgaggaaac actcgcagca ctgcaacaca
     5461 acacgtctct tttggacgtt gcgctgggtc ccgtggggga cggaattgga tccaactcct
     5521 gggccatctc tggctcgcgc acctcgactg gtttaccatt gctcgcaaat gatccccatc
     5581 tgagcatcca aatgccatcc atctggtatc aggcccattt ggaatgtaaa cccgtcagtg
     5641 aggcttgtcc gtataacgtc gccggcttca cctttgccgg tgttcccggt gtcattatcg
     5701 gccacaacca acggattgcg tggggactta ccaacgtcgg cccggatgtg atggatttgt
     5761 acatcgaacg agtcaacccc gataacccga atcaatacga ggtcaacggc cagtgggtgg
     5821 atttcgaaac ccgtaaagag gtcatccatg ttgctggggg tgaccctgtt gaaattaccg
     5881 tacggtcaac acgtcacggc ccggtgatct ctgaaaccta tggtccccta aagaacgaaa
     5941 acacaaatga tgatccgaag tttatcccgt tcaaagagcg agttggcatt gatttgccag
     6001 agcaatatgt tatctccctc aaatggacgg cgctttcgcc atcgacgccg tttgaagctg
     6061 tgtggggctt taacctggct cagaactgga aggagttccg tgaggccgcg cagcattttc
     6121 atgtgcccgc gcagaatttg ctctatgcag atgtggaagg gaatatcggc taccagatgc
     6181 caggtgatat tccgatccgt gccaacgggg agggtacatt ccctgtgcca ggttggacag
     6241 atgaatacga atggacagat ttcattccct tcgaggagtt gccgtttacc tataaccctc
     6301 cggaaggtta tattgtcact gcaaataacc aggtgccgcc aagagattat ccctatctga
     6361 tt
//
LOCUS       DASTJP010000534         5109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_331798, whole genome shotgun sequence.
ACCESSION   DASTJP010000534 DASTJP010000000
VERSION     DASTJP010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331798"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1227)
                     /gene="pseB"
                     /locus_tag="VFQ23_15635"
     CDS             complement(217..1227)
                     /gene="pseB"
                     /locus_tag="VFQ23_15635"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HET9908083.1"
                     /translation="MDWKDKVVLITGGTGSFGKKFTKILLEEKQPKKVIIFSRDELKQ
                     HEMKVNGYDHPSLRYFIGDVRDRERLNRAMHGVDIVVHAAALKQVPACEYNPMEAIKT
                     NIMGTANVLEAALDGGVQKVMTISTDKAVSPVNLYGATKLAAEKLTVQSNAYAAGTAT
                     RYSCVRYGNVVGSRGSVVPLFLNQRASGTFTITDDRMTRFWLSLEQGVRFVMSCIEQM
                     EGGEVFVPKIPSTKVVDLARAIVADARINIIGIRPGEKLHEDLVSDDEARHTIELEKM
                     YVIQPAEATWFGYSWKDKGRQLPEGYHYSSDTNTEWLDIDGIKAFVAPFEKLFAEGKL
                     EG"
     gene            complement(1307..2128)
                     /locus_tag="VFQ23_15640"
     CDS             complement(1307..2128)
                     /locus_tag="VFQ23_15640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9908084.1"
                     /translation="MENTKQQVRDFYDQIGWSQVGEGLYQNARYEDLRPVSREYIHKC
                     HMRVKRHLAPQGDILLDAGSGPVQWPAYLEYSENYRYRLCADISITALKEARKRLGDK
                     GLFVVADIANLPFKPDAFDGVVSMHTIHHLPLSEHKRAYVELNRVLKAGKKAVVVNGW
                     HNPLLMRMAEPWIGLARFFSGRTAKRKKNWSAEEDQAGTFVEKMTPRWLKNELKDSIR
                     FEIYPWRSLSPRFMRWFVRTKFGGKLYLRLIFWLEERFPRFFGENGQYPLIVIKK"
     gene            complement(2131..3747)
                     /locus_tag="VFQ23_15645"
     CDS             complement(2131..3747)
                     /locus_tag="VFQ23_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908085.1"
                     /translation="MVIKDILGNIPYSADLYDSIRKGRPRTRYNLEQLASHLPAAVEQ
                     ARPFIEKAKPGKKLLLFATLHYWIEQAAIVGLALRGLGHDVTIAYLPYHDWRKDINKF
                     DLRRQDLYTRRVLSPLDKIIHYASLLDLKPAPVLPSALTEDIALVSVYDSQYTDQIED
                     VDKNSAVYKLRLQRNDFAARAALSLMQELKPDAVLIPNGTITELGAVYRVARHLDLRT
                     ITYEFNDQREQVWIAQDDEVMRQNTDSLWKARGGKKLSAEERELIEELESARMGGRAF
                     GKSVRKWQDVEAKGGENLREELKLDNTRPIVLLATNVLGDSLTLGRNIFASSMADWIE
                     KTVRFFGAKPEVQLVIRIHPGERLTHGPSMADVVKGALGSQMPENIHLIGPLEKINTY
                     DVMEITDLGLVYTTTTGLEMAMNGIPVIVCGETHYRKRDFTLDPTSWDEYYEMIDNSL
                     KDKKSLSETQIETAWEYAYRFFFEYPLPFPWRLMHFWKDMDAWPMSRVLSDEGQIEFG
                     KTFAYLAGEPIAWTSPPSPLPVEEGRRGDG"
     gene            complement(3754..4074)
                     /locus_tag="VFQ23_15650"
     CDS             complement(3754..4074)
                     /locus_tag="VFQ23_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908086.1"
                     /translation="MNENLPISLLNWLDRWTPSVERWISAEEKVQISLQDSKQHDWKQ
                     IVYEMANDMQDISTAFAEGQDLEIPESPEGTIIMKLAFDGGFKYLNGIIQPILRKDYQ
                     HLLQ"
     gene            complement(4192..>5109)
                     /locus_tag="VFQ23_15655"
     CDS             complement(4192..>5109)
                     /locus_tag="VFQ23_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HET9908087.1"
                     /translation="GRENIYMNGAILGMKRTEIEKKFDEMVEFSEVSQFIDTPVKRYS
                     SGMYLRLAFAVAAHLEPEILVVDEVLAVGDAEFQRKCLGKMNDVAQQGRTVLFVSHNM
                     SAILRLTQEAIVLKKGQLIKRAPTPEAVDYYLASGQAESGERVWDVEDIPAASAPFRP
                     VAIRVKDRSGKVVDTVRSTEPVTVEWEYQLDSPVTGLRVGMYLNTMRGEYVFTAFDTD
                     DAQQYEQFGARPAGRYISRCNIPADFFNEGRYSLGVNASSFGVKRYFMDENALSFNVD
                     ISGAPGTQWPEIRQGPIRPRLAWTIEKID"
BASE COUNT         1226 a         1352 c         1189 g         1342 t
ORIGIN      
        1 ttagcgtacc aatcgcatcc gtttgactta agtctgctcg aatgaccgcg aaaggcgcag
       61 attgcagctt gccgcgatcc acaccgacga cttcatgctc gcgcatggct tccatcgcga
      121 ggttgattcc aagtagcccg cttgcaccag tgacgagaag tttcatcata ttcatctaga
      181 cctgacaggt ttccgaaaac ctgtcaggtc tgaacgttat ccttctaatt ttccttcggc
      241 aaacaacttt tcaaagggcg cgacgaaggc cttgatgcca tcgatatcga gccattcagt
      301 gtttgtgtcg ctcgagtagt ggtatccctc agggagctgt ctgcctttat ccttccagga
      361 ataaccaaac catgttgctt cagcgggttg aatgacatac atcttctcga gctcgatggt
      421 atggcgggcc tcgtcatcag agacaaggtc ctcatgcagc ttttcacccg ggcggatgcc
      481 aataatgtta atcctggcgt cagccacgat cgctctcgca aggtcaacga ctttggtgct
      541 gggaatcttg gggacgaaga cttcacctcc ttccatctgt tcgatgcagg acatgacaaa
      601 gcggactccc tgttccagtg agagccagaa acgggtcatg cggtcatccg taatggtaaa
      661 tgtgccactt gcacgctgat tgaggaacag tggaacgacc gaaccgcggc tcccgaccac
      721 attcccgtat cggacgcatg aatagcgggt cgccgtccct gcagcatagg cgttgctttg
      781 gaccgttaat ttttcagcag cgagtttcgt cgcgccatac aggttcacgg gactcacagc
      841 tttatctgtg ctgatcgtca ttactttttg cacacctcca tccagcgcgg cttccaacac
      901 attggcggtg cccatgatgt tggtcttgat ggcttccatc gggttgtact cacaggcagg
      961 cacctgcttc aaggcggcag catggaccac gatatccacg ccatgcatgg cgcggttgag
     1021 acgctcccta tctcgcacat caccaatgaa atagcgcagt gatggatggt catatccatt
     1081 caccttcatt tcatgctgct tcaactcgtc gcggctgaag atgatgacct ttttcggttg
     1141 tttctcttct aaaagaattt tggtgaattt ctttccaaat gaacccgtac caccggtgat
     1201 gagcacaact ttatctttcc agtccatgaa agctccttgt tctaaacggt ggtcgagtag
     1261 cccgaggcga tagccgaggg catatcgaga ccaccggttt agtaaattat ttcttaatca
     1321 caatcaacgg atactgccca ttttcaccga agaaacgcgg aaagcgttct tcgagccaga
     1381 agatcaaacg taaatacaat ttgccaccaa atttggtgcg tacgaaccag cgcataaaac
     1441 gcggactcaa actccgccac ggataaattt caaatcttat tgaatctttt aattcgtttt
     1501 tcagccagcg tggggtcatt ttttcgacga aggttcccgc ctgatcttcc tccgcgctcc
     1561 aattcttttt ccgctttgca gtgcgtccgc taaaaaagcg agccaggcca atccacggtt
     1621 ctgccattcg catcaaaagc ggattatgcc acccgttgac gactacggct tttttgcctg
     1681 cctttaagac tcgattcaac tcaacatacg cgcgtttgtg ttcactgagt ggcaaatgat
     1741 gaatcgtatg catggacaca acgccatcga acgcatctgg cttgaacgga aggttggcaa
     1801 tatcggcaac aacaaataaa cccttatcgc caagcctctt gcgggcttcc tttagcgcgg
     1861 tgattgaaat atccgcgcac aagcggtaac gataattttc ggaatattcc agataagcgg
     1921 gccattgcac aggtccagag cccgcatcca gcaagatatc gccctggggt gcaaggtgtc
     1981 ttttcacgcg catgtgacat ttatgaatat actcgcgcga aaccgggcgc aggtcttcgt
     2041 agcgggcatt ttgatataac ccctcgccaa cctgcgacca gccgatctga tcataaaaat
     2101 cacgcacctg ctgttttgtg ttttccataa ctatccgtca ccccttcgcc cctcttctac
     2161 tggcagagga gatgggggtg aggtccacgc aattggctcc ccagccaaat acgcaaacgt
     2221 cttaccgaat tctatttggc cttcatccga caatacccga ctcatcggcc aggcatccat
     2281 atccttccaa aagtgcatca gccgccaggg gaacggcagc ggatattcaa aaaagaagcg
     2341 atatgcatat tcccaggcgg tttcaatttg ggtctcgctc aaactctttt tatcctttag
     2401 agagttgtcg atcatctcat aatattcatc ccaacttgtg ggatcaagcg tgaaatcgcg
     2461 tttgcgataa tgcgtctcac cgcacacgat caccgggatt ccgttcattg ccatttcgag
     2521 tcccgtggtt gtcgtatata ccaaacccag atctgttatc tccattacat cataggtgtt
     2581 gatcttttcc agaggaccaa tcagatggat gttttcaggc atctgcgatc ctaacgcgcc
     2641 tttcaccaca tctgccatgg atggtccgtg tgtaagcctt tcgcccgggt ggatacggat
     2701 caccaactgg acctcaggct tggctccaaa aaacctgact gttttctcta tccaatctgc
     2761 catgctgctc gcaaagatat ttcttccgag ggtgaggcta tcgcccagga cgttggttgc
     2821 cagcaagacg ataggacgtg tgttatccag tttcaattcc tcacgaaggt tctcaccgcc
     2881 tttggcttct acatcctgcc atttacgcac agatttgcca aacgcccgcc cgcccatccg
     2941 tgctgattcc agctcttcaa tcagttcacg ttcctctgcg gataatttct ttccgccccg
     3001 cgccttccaa agtgagtctg tattctggcg catcacttca tcgtcctgcg cgatccagac
     3061 ctgttcgcgc tggtcattga attcgtaagt gatagtgcgc aaatccagat gacgcgccac
     3121 acgatagact gcaccaagct cggtgatcgt tccgttgggt attagaactg cgtctggttt
     3181 aagctcttgc atgagcgaga gcgcggcacg ggcggcaaag tcattgcgtt gaagacgcag
     3241 cttatagacc gcggagtttt tatccacatc ttcgatttga tctgtgtatt gcgagtcgta
     3301 gacagagacc agagcgatgt cttcggtaag agctgatggt aaaacgggag ccggtttgag
     3361 atccagtaag gaggcgtaat gaataatctt atcaagcgga gacaagactc gccgagtgta
     3421 taaatcctgc cgtcgaagat caaatttgtt gatatctttc cgccagtcgt gatatggaag
     3481 ataggcgatc gtgacgtcgt gtcccagacc gcgcagtgcg agtcccacga tggcagcctg
     3541 ttcgatccaa taatggagcg tggcaaagag cagaagtttt tttccgggtt tggctttttc
     3601 aataaacgga cgcgcctgtt cgactgcggc cggcagatga ctggcaagct gctcaaggtt
     3661 atagcgcgtg cgcggacgac ccttgcggat cgaatcatag agatcggcgg aataaggaat
     3721 attgcccaga atatctttaa ttaccatata cttttactgt aacaagtgtt gataatcctt
     3781 gcgaaggatt ggctgaataa taccgtttaa gtatttaaag ccaccatcaa aggctagttt
     3841 cattattatg gtgccctcgg gtgattcagg gatttcaaga tcttgtcctt cagcaaaggc
     3901 cgttgagata tcttgcatat catttgccat ctcgtaaaca atttgtttcc aatcatgttg
     3961 cttactgtct tgaagcgaaa tttgtacctt ttcttcagca gaaatccatc tttctaccga
     4021 tggtgtccat ctatcaagcc agttcaacaa gctgatcggc aaattctcat tcatttgctt
     4081 cgcaagtcgc gatgcaccct taattgaata aagtggcgtt cgaatgtgaa agagaaaatt
     4141 gtgcagtaaa tctgagtcgc ttaaagagct catcactagc ttttatcttt tttaatcaat
     4201 cttttcgatc gtccaagcca gacgcgggcg gatgggtccc tggcgaattt caggccattg
     4261 cgtgccgggc gcaccggaga tatccacatt aaacgacagc gcgttctcat ccatgaaata
     4321 acgcttcacg ccaaaggaac tggcattgac acccagcgag taacgacctt cattgaaaaa
     4381 atcggcaggg atgttgcagc gactgatata tcgccctgcc ggacgcgccc cgaattgctc
     4441 atattgttgc gcatcgtctg tatcgaacgc ggtaaataca tattcgccgc gcattgtgtt
     4501 gagatacatg cccacgcgca gacctgtaac cggcgaatca agctggtatt cccactcaac
     4561 cgtgacaggc tctgtagagc ggacggtatc tacgactttt ccacttctat ctttgacccg
     4621 aatcgcgaca ggtcgaaatg gagcgctcgc ggctggaata tcttccacat cccaaacgcg
     4681 ctcgccagac tcagcttgcc cggaggcaag ataataatcc actgcctcgg gcgtgggtgc
     4741 ccgtttgata agctgtcctt ttttgagaac gatggcttcc tgggttaatc gcaaaattgc
     4801 ggacatattg tgacttacaa aaagcacagt acgtccctgc tgagcgacgt cgttcatctt
     4861 gccgaggcac ttacgctgga actctgcatc gcccactgcc agaacttcat ctacaacgag
     4921 gatttccggt tcaaggtgcg cagccacggc aaacgccagc cgcagataca taccggatga
     4981 atagcgcttt actggcgtat caatgaactg actgacttca gaaaactcca ccatctcatc
     5041 gaatttcttt tcgatctctg tgcgtttcat tcccaggatc gcgccgttca tgtagatgtt
     5101 ttctcgacc
//
LOCUS       DASTJP010000535         5660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1299589, whole genome shotgun sequence.
ACCESSION   DASTJP010000535 DASTJP010000000
VERSION     DASTJP010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299589"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1196
                     /locus_tag="VFQ23_15660"
     CDS             171..1196
                     /locus_tag="VFQ23_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908088.1"
                     /translation="MNNNKPLLEVKGLKTYFYTEDGVVRAVDGVNFEVYPGEVLGLVG
                     ESGCGKSVTSLSIMRLISKPGKVDAGEILLDGENLLNLSEEEMTKVRGNRISMIFQQP
                     QTALNPVFKVGDQLAEVLNVHQDMGKDAGWKRAVALLKMVGVPDPERRVEAFPHELSG
                     GMAQRVMIAMALACVPELLIADEPTTALDVTIQAQILDLMRDMRKEMGTSVILITHDL
                     GVVAEMAERVAVMYAGEIVEQTDVNSLFDEPLHPYTQGLIGSIPVLGEIKDKLDVIPG
                     SVPNLVNLPPGCRFAPRCQSRVKYGLNICTEVKPELEEVKSDHYVRCWLYESAEEHGH
                     TAPLKVG"
     gene            1223..2236
                     /locus_tag="VFQ23_15665"
     CDS             1223..2236
                     /locus_tag="VFQ23_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908089.1"
                     /translation="MQPKPSSANHKGETLVEVNNLVKYFPVRAGLLQRVVNHVKAVDD
                     VSFAVKQGETLGLVGESGCGKTTVGRTMLRLVEPTSGAVKFGGRDVFSMKPQELKAVR
                     RDMQIIFQDPYASLDPRVPIGESVMEGLHIHRIGSPKERVDIMLETLKKVGLEDYHAR
                     RYPHEFSGGQRQRIGIARALALRPKFIICDEPVSALDVSIQSQVLNILKDLQSEFGLT
                     YLFIAHNLSVVEHVSSRVAVMYLGKMVEMASREDLFRNPLHPYTQALMSAIPVPNPRL
                     KRERIILKGDVPSPLNPPKGCRFHPRCPWAIEICSQQEPEFKELMPGHWTACWVAEQN
                     LKS"
     gene            2254..3360
                     /locus_tag="VFQ23_15670"
     CDS             2254..3360
                     /locus_tag="VFQ23_15670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9908090.1"
                     /translation="MAAPCVLLVDDQKDIVRLLHSSLQTLGHQLDLVDAPSGEEALLE
                     ASRRKIDLLVADYLLPGISGVELMRKIKARNADLKVIFISGMTERKARSEMLGAGAVA
                     IFDKPIPLADFLDAVERSLGLVRTIFPPETSKDVEEHRQTLSELLTGFRQKVKADAVF
                     LISDRARVLARAGDLYDSSMEVSMLSALMAIYSAGLKVSRFIHQERLDSYHVFRGGDH
                     DLILIPVDASHALLLAGKELANSERMLQTVEGMLFVRGDVENILQTLGVSPSTTVTEA
                     VTTAGDLNEIQETSSMFESNEPEPEMDLDALFASANTKAKVDDLDAFWNDAVEKTGNI
                     PTNPDVITYEEASKLGLTPGKGTGRLTKTGSLKK"
     gene            3428..3501
                     /locus_tag="VFQ23_15675"
     tRNA            3428..3501
                     /locus_tag="VFQ23_15675"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3460..3462,aa:Gln,seq:ttg)
     gene            3601..4971
                     /gene="glmU"
                     /locus_tag="VFQ23_15680"
     CDS             3601..4971
                     /gene="glmU"
                     /locus_tag="VFQ23_15680"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET9908091.1"
                     /translation="MKVTAVLLAAGQGTRMKSDLPKVLHPICGRPMIWHVLRALNSAA
                     TEKPVVVVGYGAEQVQEYLGDSADCVLQEHQLGTGHAVMQAESVLRGKTDYVIVAYAD
                     MPLLRGEAFNRLVETQRHNPGPLSLVTAIADDPRGFGRILRKPDGTVEAIVEEYVATP
                     EQRKIKELNVGAYCFKADWLWDALHRIQKNPRKGEYYLTDIVEVAVQDNLPVQPVIQH
                     NLAETIGINTRVHLSEAEAAMRLRINREYMLDGVSMTNPASAYIEIGVKIGKDTVIFP
                     NTHIQGDTEIGEGNVIGPNTIIRDSKIGNRCKILASVVEGAVIEDDVDMGPFARLRKG
                     AHLKSHVHMGNFGEVKDSTLHEGVKMGHFSYIGNAIVGANTNIGAGTITANYDGEKKN
                     ATEIGEDAFIGSDTMLVAPLKLGDGARTGAGAVVTKNVPDDTLVVGMPARAIRKLERK
                     NKKNHH"
     gene            5027..5437
                     /gene="cdd"
                     /locus_tag="VFQ23_15685"
     CDS             5027..5437
                     /gene="cdd"
                     /locus_tag="VFQ23_15685"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET9908092.1"
                     /translation="MTITEQEKQALIDLANTARQRAYVPYSHYPVGAALRTKSGRIFT
                     GVNVENAAYPHTMCAERVAIFKAVSEGEQDFEIISVVTDNGGSPCGGCRQVLSEFGMD
                     TIVLMADGSGKLVKETTVKELLPEAFTPDHLLNK"
     gene            5481..>5660
                     /locus_tag="VFQ23_15690"
     CDS             5481..>5660
                     /locus_tag="VFQ23_15690"
                     /inference="COORDINATES: protein motif:HMM:NF023393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HET9908093.1"
                     /translation="MPETKFRSFRVEAVVLRHHDYGEADRLLTLYTRQLGKTRVLAKG
                     ARKIASRKAGHIEPFT"
BASE COUNT         1456 a         1306 c         1515 g         1383 t
ORIGIN      
        1 aaacgtttga ttatggaacg ggttgcaatc cgtgagggac aaccgagcct tcgaatacgt
       61 gcccgcctct cttaaatcaa cattgatctc cctttggtct ctgtttgacc gtataaaata
      121 aaaaattttg ctataatgca aagaatcctt gcatatcgga gaatccggcg atgaacaaca
      181 acaaacctct gttggaagtt aagggtctca agacatactt ttacaccgaa gatggtgttg
      241 ttcgcgcggt ggatggtgtg aactttgaag tttatccggg ggaagtatta gggctggttg
      301 gtgaatccgg ttgcggcaaa agtgttacat ccctctccat catgcgcctg atttccaaac
      361 caggaaaagt ggatgctggt gagattctcc tggatggcga gaatcttttg aatttatcag
      421 aagaggaaat gacaaaggtg cgtggcaatc gcatctcgat gatcttccaa caaccgcaga
      481 cggcactgaa ccccgtattc aaagtgggag accaactggc tgaagtatta aatgttcatc
      541 aggatatggg gaaagacgcg ggctggaagc gggcggtcgc gttactcaag atggttggtg
      601 ttccagatcc cgagcgccgt gtagaggcgt ttccgcatga attatcaggt ggtatggcac
      661 agcgtgtcat gatcgccatg gcactggctt gcgttccgga attgctgatt gctgacgaac
      721 caactacagc tctggatgtg acgattcaag ctcagatcct ggatttgatg cgcgatatgc
      781 gcaaggagat gggtacctct gttatactca tcacgcacga tctgggtgtg gtcgctgaaa
      841 tggctgaacg agtagcggtc atgtacgcgg gcgaaattgt tgagcaaaca gacgttaatt
      901 ccctgttcga tgaaccgtta cacccctaca ctcagggctt gattggctcc atccctgtcc
      961 ttggtgagat caaagacaaa ctcgatgtta ttcccggctc tgttccaaac ttggtgaacc
     1021 ttccgccggg ctgtcgtttt gcgccgcgtt gtcagtcgcg cgtcaaatat ggattgaaca
     1081 tctgtactga ggtaaaacct gaactggaag aggtcaagtc tgaccattat gtacgctgct
     1141 ggctatatga gagcgcggaa gagcacggac atactgctcc gctcaaagtc ggctagccat
     1201 cccatttttg gagaatacaa acatgcagcc caaaccctct tccgcaaatc acaaagggga
     1261 aaccctggtt gaagttaata atcttgttaa atatttccct gtgcgtgccg gtttattgca
     1321 gcgtgttgtt aatcacgtca aggcagtgga tgacgtcagc ttcgccgtaa agcaagggga
     1381 aacattgggg ttggtgggag aatccggttg tggaaagaca acggttggac gcaccatgct
     1441 tcgcctggtt gagccgacct cgggagcagt aaagttcggt ggcagagatg ttttttccat
     1501 gaagccccag gaacttaagg cggttcgccg tgacatgcag atcatctttc aggatcccta
     1561 cgcctcgctg gatccgcgcg tgcccattgg cgaatcggtg atggaaggcc tgcacatcca
     1621 ccggatcgga tcaccgaaag aacgcgtcga tatcatgcta gagacgctta agaaagtagg
     1681 tttggaggat tatcacgctc gccggtatcc gcatgagttt tcaggggggc aacgtcagcg
     1741 tattggaatt gcacgggcgc tggcacttcg tccaaaattt attatctgtg atgaacccgt
     1801 ctctgcgcta gacgtatcca ttcagtcaca agtcttgaat attctgaagg atttacagtc
     1861 tgaatttgga cttacatatt tattcattgc acataatctt tccgtggtgg agcatgtatc
     1921 cagtcgcgtg gcagtgatgt atttgggaaa aatggtcgaa atggcctcac gtgaagactt
     1981 attccgtaat cccctgcatc cctatacaca ggcattgatg tctgcgatcc ctgttccaaa
     2041 cccgcgtttg aaaagagagc gcattattct gaagggggat gtacccagcc cactcaaccc
     2101 gccaaaaggt tgccgtttcc atcctcgctg cccctgggca attgaaatct gttcgcagca
     2161 ggaaccagag tttaaggaat taatgcctgg acactggacg gcttgctggg tggctgagca
     2221 gaacctgaaa tcttaaccag cccgctgaaa tttatggctg ctccgtgtgt tcttctggtt
     2281 gacgatcaaa aagacatagt ccgtcttctc cattcaagtt tacaaacctt aggtcaccaa
     2341 ttggatctgg tcgatgctcc ttctggcgaa gaggcattgc tggaggcctc gcgtcgcaaa
     2401 atcgatttat tggtggcaga ttatcttcta cccggtatct ccggcgttga gttgatgcgc
     2461 aagatcaagg cgcggaatgc agacctgaaa gtcatcttca tctctggcat gaccgagcga
     2521 aaagcgcgtt ccgaaatgct gggggctggg gcggtcgcta ttttcgacaa acccataccc
     2581 ctggcagatt ttctcgatgc ggtggaacgc agcctgggtt tggtccgtac gatcttcccc
     2641 cctgaaacca gcaaggatgt agaagaacat cgtcagactc tttcagagtt gttgaccggt
     2701 ttccgccaaa aagtaaaggc agatgccgtc ttcctgatta gcgaccgtgc acgcgtgttg
     2761 gcacgcgccg gggacttata tgacagcagt atggaagtct caatgctctc ggcgttgatg
     2821 gcgatttaca gcgcagggct caaggtctcc cgtttcatcc accaggaaag gttggatagc
     2881 taccacgtgt ttcgcggcgg cgatcatgat ctaatcctta tacccgtaga tgcatcccat
     2941 gcattattgc tggccggtaa ggaacttgcc aattctgagc gtatgcttca aacggtggaa
     3001 ggcatgctct tcgtgcgcgg tgatgtggaa aatatcctcc aaacactggg ggtgtcacct
     3061 tcgacaacgg taacggaagc cgtcaccact gcgggcgatc tgaacgaaat ccaggaaacg
     3121 tcgagtatgt ttgaatccaa tgaacccgag ccggaaatgg atctcgacgc gttgttcgca
     3181 tctgcaaaca ccaaggcaaa agttgatgac cttgacgcgt tctggaatga cgcagtggaa
     3241 aagaccggca acattccgac aaaccctgat gtgatcacct atgaagaagc cagtaaactt
     3301 ggcttgacgc ccgggaaagg tacgggacgg cttacaaaaa caggttcact taagaagtga
     3361 cacaccgtac ctccgcacgg tgcaggtgtt gcccatccct aatcgctatg atacaattcg
     3421 tgcgttctgg ggcgtggtgc aagggcagca caccagcctt tggagctgtg gatcttggtt
     3481 cgaatccagg cgccccagcc atcgccgatt ataggcatcc cgcgtaggcg gggcttattt
     3541 tctaaacagg cttcgcttgc ataacgcagt aagcctgttt ttattcggag taattgattt
     3601 atgaaagtga ctgctgtctt gttagctgcg ggtcagggaa ctcgcatgaa atcggatctg
     3661 cccaaggtgc tgcatcccat ctgcggcagg cctatgattt ggcacgtgtt acgagcatta
     3721 aattcggcag cgaccgaaaa gcctgttgtc gttgttgggt acggagctga gcaggtacag
     3781 gaatatctgg gtgactcggc ggactgtgtt ttacaggaac atcaattggg gacaggacat
     3841 gccgtcatgc aagcggaatc cgttctaaga gggaaaacag attatgtgat cgtggcgtat
     3901 gcagatatgc ctcttttgcg aggggaagcc ttcaatcgac tcgtggaaac gcagcgccac
     3961 aaccctggac ccttgagttt ggtgaccgcc atcgcggatg acccgcgtgg ctttggccgg
     4021 attctgcgca aaccggatgg gactgtggaa gccattgttg aagaatatgt tgcaacacct
     4081 gagcaaagga aaatcaaaga gctaaatgtg ggggcatatt gcttcaaagc tgactggtta
     4141 tgggatgctc tacatcgtat tcagaaaaac ccgaggaagg gtgaatatta tctaacggac
     4201 attgttgaag ttgcagtaca ggataatttg ccagtccagc ctgtaattca acacaacctg
     4261 gctgagacca ttggtatcaa tacccgcgtt catttgagtg aagcagaagc agccatgcga
     4321 ctccgcatca accgcgaata tatgttggat ggcgtgagca tgaccaaccc ggcttctgca
     4381 tatattgaga ttggcgtcaa aattggtaag gatacggtca ttttcccgaa tactcatatt
     4441 cagggtgata cagaaatcgg cgaagggaat gtgatcggtc cgaacactat cattcgagat
     4501 tcaaaaattg gcaatcgttg caaaatattg gcatctgttg tggaaggtgc cgtgattgaa
     4561 gatgatgtgg atatgggacc atttgcacgc ttgcgaaaag gtgcacacct gaagagtcac
     4621 gttcacatgg ggaatttcgg tgaggtgaaa gattccacct tacatgaagg cgtgaagatg
     4681 ggtcattttt cgtatattgg caacgcgatt gtcggcgcca atacgaatat cggagctgga
     4741 accatcaccg cgaactatga tggtgagaaa aagaatgcaa ccgaaatcgg cgaagatgcg
     4801 tttataggtt ctgatacgat gctggttgca ccgctgaaac ttggggatgg agcacggacg
     4861 ggcgcgggcg cggtcgtgac caaaaacgta ccggacgata cgctggtcgt tggtatgcca
     4921 gcaagagcca tcagaaaatt agaaagaaag aataagaaaa atcaccactg agccgcggaa
     4981 acaaagagaa aaaatctctg agtctccgtg gcaaacaggt tgctttatga caatcactga
     5041 acaagaaaaa caagccctca ttgatctcgc aaatacggca cgccaacgtg cctatgtgcc
     5101 ctactcccac tatccggtcg gcgcggcact tcgtacaaaa agtgggcgca tcttcacagg
     5161 ggttaacgtg gagaatgcgg cgtatccaca cacgatgtgt gcggaacgtg ttgccatctt
     5221 caaagcagtt tcagaggggg agcaggattt tgaaattatc tctgtggtaa ccgataatgg
     5281 tggttcaccc tgcggtggat gtcgtcaggt gctgtctgag tttggtatgg ataccattgt
     5341 gctgatggcg gatgggagtg gtaaacttgt taaagaaacg acagtgaaag aactattgcc
     5401 cgaggcattt acaccagatc acttgctaaa taaataaata ttcaccttta aacgaccgtt
     5461 acctttaaac atttcatatc atgcctgaaa ccaaattccg ttcatttcgc gtagaagccg
     5521 ttgtgcttcg tcatcatgat tatggggaag cagatcgcct gttgacgctc tacacccgcc
     5581 agcttggaaa aacacgagta cttgccaaag gtgcgcgcaa gatcgcctcg cgcaaggccg
     5641 ggcatattga gccattcaca
//
LOCUS       DASTJP010000536         4436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_700573, whole genome shotgun sequence.
ACCESSION   DASTJP010000536 DASTJP010000000
VERSION     DASTJP010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700573"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFQ23_15695"
     CDS             <1..890
                     /locus_tag="VFQ23_15695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9908094.1"
                     /translation="YARVAVLFKDVTERKRAEEELQQLNLQLESRVQRRTGELQTANQ
                     SLLAEIASRQRVEDALQESRRRLQMLSQRLVDVQEEERRAIARELHDRVGQSLSALNI
                     NLVILDNQISDRIGEPIRSRLDDSMQLVAETITLVRDVMSDLRPPVLDDYGLEAALQS
                     HLSAFKSRYGIDVLFEKPEQPIPRLSASIEMTFVRIAQEALMNVARHAKAKQVTLSLM
                     QDEKMVRLIIQDNGEGIRSWQEANRPGSHGLTIMRERAEAFGGNLRVSSIPGKGVTVD
                     ASIPIDSDEPENAQKERSE"
     gene            887..1285
                     /locus_tag="VFQ23_15700"
     CDS             887..1285
                     /locus_tag="VFQ23_15700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908095.1"
                     /translation="MITVVLADDHTVLRDGLRYLLEAAGGIEIVSTASNGQEAVEQAS
                     LHCPDVVLMDISMPIKNGIEATKEIREACENTKVAILSMHHTSEYVQRAMQAGAHGYV
                     LKDSAGSEVIAAIHAIHEGKRYFSQKIAGM"
     gene            1584..1994
                     /locus_tag="VFQ23_15705"
     CDS             1584..1994
                     /locus_tag="VFQ23_15705"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HET9908096.1"
                     /translation="MTTKADEEFVKDGAEKITEKDIEKVVDKSEDIKQKFASRGPLKR
                     FIEDAKLLLAFIKDYRSSKYRQVPYGIIAAAVFALLYVFNPFDLVPDVLPFVGAVDDA
                     TVIGACLLMMEKDLNKFRKWKEGQNQNQIEGNTE"
     gene            1991..2398
                     /locus_tag="VFQ23_15710"
     CDS             1991..2398
                     /locus_tag="VFQ23_15710"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET9908097.1"
                     /translation="MKKKADVQISGFTRFAKAASRATGKPATFIGAALLILLWAITGP
                     IFDFSDTWQLFINTSTTIVTFLMVFLIQNTQNRDTEAMHIKLDELIRAVGTAQNTMLD
                     LEELDEKELDEIRAEYEKLAEKARKKLKSTRSK"
     gene            complement(2404..4101)
                     /locus_tag="VFQ23_15715"
     CDS             complement(2404..4101)
                     /locus_tag="VFQ23_15715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9908098.1"
                     /translation="MTTEFTSLNLREEVVQAITGLGYAEPTPIQTAIIPIMLTGADVI
                     GQAQTGTGKTAAFALPILHNFTPQRHVQALILAPTRELALQVADSMTEYGKHLNVRVL
                     AVYGGQPYGPQISRLNRGVDIVVGTPGRLLDLIKRNALNIKHIRTVVLDEADEMLNMG
                     FIEDVEEILGDTPPERQTALFSATLPPRIRTLANRFMRDPQSVNIKRSTLTLASTEQR
                     YYLVHESDKTNALTRLFEIEPIKSALIFARTRAETATLANELVVRGIPAEAIHGDLDQ
                     NARERVLGRFRANQLKVLVATDVAARGLDIEDISHVFNYHLPDDAEVYIHRIGRTGRA
                     GKTGVAITLLSPREKRRMREVEALTKQPVTKVELPTIEDIHRHREAEVVENVKIWLGR
                     GRFKRELEMVNELVQAGHDPLNVAAAALKIARADEKQRPIAEIADVKSDRREKDVKPR
                     GDRRETPERRNKPAKGGSSRRRGDWSHEEGMIRLKLNKGKLNGIRPNDIVGQIAFHAN
                     IPGYTIGKIRIEDKQSFVDIPEDLVEQVLKHNGNYGIGKEKFNLQKTNRRTDCSSHL"
BASE COUNT         1098 a         1053 c         1193 g         1092 t
ORIGIN      
        1 cgtatgcgcg tgtggcggtc ctcttcaaag acgtcacaga acgcaaacga gccgaagaag
       61 aattacagca gctcaacctt cagttggaaa gccgtgtgca gagacgtact ggtgagctcc
      121 agacggcaaa ccagtcccta cttgcagaga tagcctcacg tcaaagagtg gaagatgcct
      181 tacaggaaag tcgcaggcgt ctgcaaatgc tttcacagcg attggtggac gttcaagaag
      241 aagagcggcg ggccattgct cgtgaactgc atgaccgcgt gggtcagagt ctttcagcat
      301 tgaatatcaa tctggtcatt ctggacaatc agatttcgga ccggatcgga gagccgatcc
      361 gctcccggct ggatgattcc atgcagctcg tagcagagac gattacgctg gtccgggacg
      421 tgatgtctga tctgcgtcct cccgtgcttg atgattatgg acttgaagcc gctctgcaat
      481 cgcacttgag cgcatttaag tccaggtatg gaattgatgt cctgtttgaa aaaccagaac
      541 aacccatccc gcgcctgagt gccagtatcg aaatgacttt cgtacgtatt gctcaggaag
      601 cgctgatgaa tgtcgctcgt catgccaaag ccaagcaggt gaccttatcc ctcatgcagg
      661 atgagaaaat ggttcgtctc atcattcagg ataacggcga ggggattcga tcctggcaag
      721 aggcaaatcg ccctggcagc catggattga cgatcatgcg cgaacgagcg gaagcgttcg
      781 ggggcaattt aagagtgtcg tcgatacccg ggaagggtgt tacagtggat gcaagtattc
      841 ccattgattc tgatgaacca gagaatgcac agaaagagag aagcgaatga ttactgttgt
      901 ccttgctgat gatcacactg ttttacgaga tggtttgcgt tatttgctcg aagctgccgg
      961 agggatcgag atcgtgtcta cagcttccaa cggacaggag gctgtagagc aagccagcct
     1021 ccactgcccg gatgttgtgt tgatggatat ctccatgccg attaagaacg gcatcgaagc
     1081 cacaaaagag atacgcgagg cctgtgaaaa tacaaaggtg gcgatccttt cgatgcatca
     1141 caccagtgaa tatgtgcagc gcgccatgca ggcgggcgcg catggttatg tattgaagga
     1201 ctctgcaggt tcggaagtca tcgcagccat tcatgcaatt catgaaggga aacgctattt
     1261 tagtcaaaaa attgctggaa tgtgaacgac gaagactcta tgaattaaat ttgtgggagt
     1321 ggctaagaga gtgagtgaaa ctctacttag ccacacacta aattcgccac tcccaatttg
     1381 ttcggctcga aattagaggg gaggaatggg atgtgactgt gtagggataa tccctatacg
     1441 gcgtttatct gtatggggat aaaattaatc aatgtcctct ctttggaaat tggcacgctg
     1501 ccaaaaaaac atctgccctg gtacgaattt agccgtacga tgccagagag gtcagggcca
     1561 gtagcgaaaa ggagcgtaat agaatgacta caaaagccga tgaagagttt gtcaaggatg
     1621 gcgcagaaaa gatcaccgag aaggatatcg aaaaggtcgt agataaatcg gaagatatta
     1681 aacagaaatt tgcttcgcgc ggaccgttaa agcgctttat cgaagatgca aaactgttgc
     1741 tggcgtttat caaagattac aggtcgagca agtatcgcca ggttccatac gggatcattg
     1801 ctgcggccgt gtttgcgctc ctctacgttt tcaatccatt tgacctggtt cccgatgtgc
     1861 tgccgtttgt cggcgcagtg gatgatgcaa cagtgatcgg ggcatgtctg ctgatgatgg
     1921 aaaaggatct aaataaattt agaaaatgga aggagggtca aaatcagaac cagatcgaag
     1981 gaaatacaga atgaagaaaa aagctgatgt tcagatatct gggttcactc gttttgccaa
     2041 agcggcctct cgtgcaacgg gcaagccagc cacgttcatt ggcgcagctc ttctcatcct
     2101 gttatgggca attacgggac ccatcttcga tttcagcgac acctggcagt tattcatcaa
     2161 cacctctact accattgtca cctttctaat ggtgtttctc atccaaaata cacaaaatcg
     2221 cgataccgaa gccatgcata tcaagctgga tgagctaata cgagccgtag gcacggccca
     2281 gaatacaatg ttggatctcg aggaactgga tgagaaagag cttgatgaaa ttcgagccga
     2341 atatgaaaaa ttggcggaga aggctagaaa gaaattaaaa agtacacgat cgaaatgact
     2401 ttgctacagg tggcttgagc aatctgttcg tcgattggtt ttttgtaaat taaatttttc
     2461 tttgccaatt ccgtaattgc cattgtgttt cagtacctgc tccaccagat cttcggggat
     2521 atctacaaag ctttgtttgt cttcaatgcg gatcttacca atcgtatagc cagggatgtt
     2581 ggcgtgaaag gcaatctgcc ccacgatatc attgggtcgg attccgttca acttgccttt
     2641 attcaacttc aaacggatca tcccttcttc atgagaccaa tcgccgcgcc gtcttgagct
     2701 gcctcccttt gctggcttgt tacggcgctc aggagtttca cgcctatcgc cacgcggttt
     2761 cacatccttt tcgcgacgat cagattttac atcggcgatt tctgcaatgg gacgctgctt
     2821 ttcatccgcg cgcgcgatct tgagtgccgc agccgcgacg ttcaatgggt catgcccagc
     2881 ctggacaagt tcattcacca tctcgagttc gcgcttgaaa cggccgcgcc cgagccagat
     2941 ctttacgttc tcaacgactt ctgcctctcg atgacgatga atgtcctcta ttgtggggag
     3001 ttccactttt gtgacgggtt gcttggtcag cgcctccact tcgcgcattc gtcgtttctc
     3061 gcgcggtgaa agtagtgtaa tggcaacgcc ggtcttgcca gcacgacctg tccgcccgat
     3121 gcggtgaatg tacacctccg catcatcggg gagatgatag ttgaacacat gcgaaatgtc
     3181 ttcgatatcc agcccgcgcg cggcgacatc tgtcgcgacc agcactttta attgatttgc
     3241 cctaaatctt ccaagcacgc gttcgcgcgc gttctggtcc aggtcaccgt ggatggcctc
     3301 ggcagggatt ccgcgcacga ccagttcatt cgcaagggtg gcggtctccg cgcgcgtgcg
     3361 ggcgaagatc aaggcacttt tgatcggttc aatctcaaag agacgcgtga gggcgttggt
     3421 cttatccgat tcatggacga gataatagcg ttgttcagtc gaggccaggg tgagggttga
     3481 gcgcttaata ttgactgact gaggatctcg catgaaacga ttcgccaggg tgcggatacg
     3541 cggcggcaga gttgcagaaa ataaagcagt ctgtctttct ggtggagtat ccccaaggat
     3601 ctcctccaca tcttcaatga agcccatgtt gagcatttca tctgcctcgt ccagcacgac
     3661 cgtgcggatg tgcttgatgt tgagcgcgtt gcgtttgatc agatccaaca agcgaccggg
     3721 tgtgcccacg acaatatcca cgccgcggtt gaggcgcgag atctgcggtc catagggctg
     3781 cccgccatag acggcaagca cgcgcacgtt caaatgcttt ccgtattcgg tcatcgaatc
     3841 ggcgacctgc agcgcaagtt cgcgcgtggg tgcaaggatg agtgcctgca catgtcgctg
     3901 gggggtaaag ttgtgaagaa tggggagagc gaaagcagcg gtcttgcctg tgccggtctg
     3961 agcctgaccg atcacatccg ctccggtgag catgatgggg atgatagcgg tttgaatagg
     4021 tgttggctcg gcatagccaa gtccagtaat ggcctgcacg acttcctcgc gcaggttaag
     4081 agaagtaaat tcggtggtca tcttttcctt tttatttatg tcattgcgag gagacttgag
     4141 cggttttgcg aaaggcatat cgagaccgag gcgataaagc aatctcatcc ttacaccaaa
     4201 ccatatgtca ggttagttga gacacttgca cctggtgcac atcgctccgg ttggtcgttg
     4261 ccggggagtg caggtgttgc cacgcctgtt tcgcaggttc gcaatgacaa acatgtagtg
     4321 gattgtttcg atgactccgt accacttctt cttgccaacc aaagcttggc ttttcagcgc
     4381 agcttgccca acaaaaaagc ccgatcaaat ttcgtcgggc attcgttcag agtgat
//
LOCUS       DASTJP010000537         7502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_20929, whole genome shotgun sequence.
ACCESSION   DASTJP010000537 DASTJP010000000
VERSION     DASTJP010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20929"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1536)
                     /locus_tag="VFQ23_15720"
     CDS             complement(100..1536)
                     /locus_tag="VFQ23_15720"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9908099.1"
                     /translation="MNQSDFNSVLPVTILTLWACVLLLVDLFVPKDRKSITALLSALG
                     LAITLGFTLTQIGLEVSGFNDMVALDGFSTFVNALLLVSGLLGIALAYGYVKRMGIES
                     GEYYTLLLFSVVGMMLIAQATDLIVVFIALELLSIPLYVLAAFARPKVESEEAGIKYF
                     LLGAFATGFVVYGTALVYGATGSTNLSAIVAFASGETSSLLLTIGAALILVGFGFKVA
                     AVPFHMWTPDVYQGAPSAVTAFMSSGAKIAGFAALLRVFATAFPSIAVDMTDIFWALA
                     ALTMILGNVIAISQTDIKRLLAYSSIAHAGYILMAFVPYGNPEVRADSIAAGLFYLVS
                     YAVTNFGAWGVVIALERQAGKGLQLNDYAGLSKRHPALAAAMAIFMLSLIGLPPTIGL
                     VGKLYLFRAVIAGGFYWLAIIGVLTSVISAYYYLRVVVIMYMRDGEPQVERESFLDLT
                     TATTALLTVILSFVPQFLFALASSAVIK"
     gene            complement(1718..3265)
                     /locus_tag="VFQ23_15725"
     CDS             complement(1718..3265)
                     /locus_tag="VFQ23_15725"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9908100.1"
                     /translation="MNFFLEPLTILTFFPLLGVLALFFIPSDNKNSLRWTALITSLIT
                     LVISIWVLTRFNASNPDLQLEARYNWIQVAGWNIQYYLAVDGLSILLVLLTTFLTPIS
                     ILSTWTSIEERVKDFMIFFLLLEIGMNGVFLAQDLFLFYIFWEFTLVPMYFIIGIWGG
                     PRRIYAAIKFFLYTMAGSILMLLAILWLGIQGQTFSVPDLAAQGNIPAGTQMWLFLAF
                     TAAFAIKVPMWPLHSWLPDAHVEAPTAGSVILAGVLLKLGTYGFLRFNLPLFPQASLQ
                     AAPWIALFAVIGIIYGAAVSYAQSDVKKLVAYSSVSHLGFVMLGLFALNHQGIAGAIL
                     QMINHGLSTGALFLLVGMIYEQTHTRELKVYGGLWKITPIFGTIMLIVALSSMGLPGL
                     NGFVGEFVILLGAWGAGTDGGALSPFGGVWFAGIAAIGIIMAAVYILYMFQKMFLGPQ
                     GEIVESVKEHGHGLRDLNWREILTIAPLLIFIFWIGLYPAPFFDLMAPSVEKLTAALQ
                     TAALAMR"
     gene            complement(3359..5518)
                     /locus_tag="VFQ23_15730"
     CDS             complement(3359..5518)
                     /locus_tag="VFQ23_15730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9908101.1"
                     /translation="MPEHVNTGFLYLAPWLVFAPVIGLLINIIFGRRFSEGMIGTVAS
                     LASGATFIVSLLLASTASAAHGEALRWYLADWIHVGTLNLDWTFRVDTLSTTMMLVVS
                     GVGTLIHIYAIGYMHEDVRFKNDVGRFRRFFVYLNLFIAAMMILVSGDSYLMLFVGWE
                     GVGLCSFLLIGFWYEMDLLMRPSWENSNAAKKAFIVNRVGDFGFMIAGFIMFWAFGSL
                     QFDAVFEGVHEVSHGVITAITLFMLIGVAGKSAQIPLYVWLPDAMAGPTPVSALIHAA
                     TMVTAGVYLITRSAPLYSAVHDAQYIVAMTGAVTALFAATIAVGQFDIKKVLAYSTIS
                     QLGFMVAAVGMGAYVAGMFHLITHAFFKALLFLSAGSVILGMERGHHHAEHAHAHHGG
                     GQGKGKKKHKEKQHASQDSHSSSAHGEVFDPGDMRNMGGLRKTMPTTFWLYMIGTLAL
                     AGIFPFAGFWSKDEILLDASLHYRSVYILLSIAAFFTAFYMGRQIWMVFFGEPRHEAA
                     AHAEESPRVMTVPLMILAVLSVLGGALNLPFEGFHNLGHWLEQTLGEVEALPLDIVVA
                     GISTVLALAALYFSWLIYGRNPLKSGQIDPLKRSLGSIFTAMENKWYVDELYQAIIVT
                     PFVKISQFLADVIDWRFWHDWFHEKVIAGTYNWLSHVALDKYADQRGIDAFANGLATV
                     TQWSSAVLRKVQNGFVRSYALSVLLGVVLIVGYLILK"
     gene            complement(5521..5814)
                     /gene="nuoK"
                     /locus_tag="VFQ23_15735"
     CDS             complement(5521..5814)
                     /gene="nuoK"
                     /locus_tag="VFQ23_15735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9908102.1"
                     /translation="MNYYIILSAILFTIGVLGVLIRRNALIIFMSIELMLNAANLALV
                     AFNSVLESFSGQIFVFFVIAVAAAEVAVGLALIVEIFRTKNSIDVDQMSSLKG"
     gene            complement(5825..6343)
                     /locus_tag="VFQ23_15740"
     CDS             complement(5825..6343)
                     /locus_tag="VFQ23_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET9908103.1"
                     /translation="MTLDLILFLVLALIAVASALGMLLSRNAVYAALFLVLNFVTVAV
                     FYLLLGAPFIAMAQITVYAGAIMVLFLFVIMLLGAESLPGISNWRNVWFPATLAAILF
                     AEAAYLLIVKARPAGDVLQPEAVVNTMENLRQLGTALFSDYLLPFEIVSILLLVAMVG
                     AIILTKKEKGVR"
     gene            complement(6387..6908)
                     /gene="nuoI"
                     /locus_tag="VFQ23_15745"
     CDS             complement(6387..6908)
                     /gene="nuoI"
                     /locus_tag="VFQ23_15745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET9908104.1"
                     /translation="MGVLDPIVEMARGLWITLKSIGEKPVTYEYPEEKRPVRPRYRGR
                     HVLKRYDNGLEKCIGCSLCAAACPSDAIFVEASENTDEKRFSPGERYASTYEINMLRC
                     IFCGYCEDACPTEAIVLGDNYELSYTSRRAAIYTKDMLLEPVPTTAMLTPQEVEPGKY
                     NRRSVPEMKDPHD"
     gene            complement(6895..>7502)
                     /locus_tag="VFQ23_15750"
     CDS             complement(6895..>7502)
                     /locus_tag="VFQ23_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET9908105.1"
                     /translation="TAIILADSMSLHDIVDRQKDMWFVVAQPVGALVFLIVTLAEVNR
                     APFDMPEAEQELTAGYHSEYSGMKFALFFMAEYQKMIIVSAIFAVFFFGGYLGPGIDR
                     FPLLGPVYMFLKIVILLFGMIWVRATWPRIRYDRLMSFGWKVLIPLSLAIVFITATGI
                     LLAEQVDPMFIWIIPVASLLVGLIPVITIYRSIRRLSYGRA"
BASE COUNT         2152 a         1985 c         1870 g         1495 t
ORIGIN      
        1 cgtgcgcagt tgaggaagtt gcgttccgca gcataaaaca aaacggggct gtggatccac
       61 agtcccgttt tgtttcgtca gatggaggac aggtcagaac tacttgatta ccgcgctgct
      121 tgccagagca aacaggaatt gtggcacaaa gcttagtatg accgtcagca atgccgttgt
      181 agcagttgta agatcgagga aggactcgcg ctcgacctgt ggctcaccat cccgcatgta
      241 catgataacg accacccgca gatagtagta ggcggagatc actgaagtga gcacaccgat
      301 gatcgccagc cagtaaaacc cacccgcgat caccgctcgg aaaagataaa gtttgccgac
      361 caaaccaatc gttgggggaa gaccgatcaa cgaaagcatg aagatcgcca tcgcagccgc
      421 aagcgcggga tgtcgcttcg agagaccggc gtaatcgttc aactgcaggc ctttgcctgc
      481 ctgcctttca agcgcaatga ctacccccca ggcgccgaaa ttagtcaccg cgtaagacac
      541 aagatagaac aaacccgccg cgatcgaatc agccctaacc tcagggttcc catacggcac
      601 gaaggccatg aggatgtagc cagcatgcgc gatgctggaa tacgccagca gtcgtttgat
      661 atcggtctgt gatatcgcaa tcacgttgcc aaggatcatc gtcagcgcag ctaaggccca
      721 gaagatatcg gtcatatcca ccgcgatgga agggaatgct gttgcaaaaa cacgcagcaa
      781 ggcagcaaag ccagcaatct ttgccccgga ggacatgaaa gcggtcacgg cggagggcgc
      841 accctgatac acatccggag tccacatgtg gaagggaact gcagccacct tgaaaccgaa
      901 ccccactaaa atgagcgccg cgccgatggt caggagcagg ctggaagtct cgcctgaggc
      961 gaatgcaaca atagcagaaa gatttgttga accagtcgcg ccatagacca acgccgtccc
     1021 atagacgaca aatcccgttg cgaacgcacc cagcagaaag tatttgattc ccgcttcctc
     1081 cgattcgact ttcggtcgcg caaatgccgc caatacataa agagggatcg aaagcagttc
     1141 gagcgcaatg aagacaacaa tcaggtctgt ggcttgcgcg atgagcatca taccgacgac
     1201 gctaaagagc agcaatgtgt agtattcgcc gctctcgatg cccatgcgtt tgacataacc
     1261 ataagcaagg gcaataccca gcaaaccact gacgagtagc aacgcattga caaaggtcga
     1321 gaagccatcg agggcgacca tatcgttgaa gccactcact tcgagcccga tttgcgtgag
     1381 cgtaaaacca agcgtgatgg ccagccccag cgcggaaagc aatgctgtga tgcttttgcg
     1441 atcctttgga acgaaaaggt caaccagcag taacacgcat gcccataggg ttagtatggt
     1501 cactggaagt acagagttaa agtcggattg attcatagat agctttcagc tacagatttt
     1561 gtttcttcct tggtcgagta ggcgatgttc gtctgccata tcgagaccac attcttgtaa
     1621 cttcacattc atttgctact tgttggtttc gatgcgggct cacctgcact gcacgcaggt
     1681 gcaagtgtca ccctactcaa ccaacggatt tccttgttta tcgcatcgcc aatgcagcag
     1741 tctgcaaagc agctgtgagt ttttccacag agggtgccat caggtcaaaa aatggagcgg
     1801 ggtacagacc aatccaaaag ataaagatga gcaagggagc gatggtgaga atctcgcgcc
     1861 agttcaggtc gcggagtccg tgcccgtgtt cctttaccga ttctacaatt tcaccctgcg
     1921 gacccaggaa cattttctgg aacatgtaaa gaatataaac agcagccatg ataataccga
     1981 tggcagcgat gccggcgaac cagactcctc caaacggaga gagcgcaccg ccatctgtgc
     2041 cggctcccca ggcaccaagc aggatcacaa attcaccgac gaatccgttc aagccaggca
     2101 aacccatcga cgagagcgcc acgatcaaca tgatcgtccc aaagatagga gtgatcttcc
     2161 acaatccgcc gtagactttg agttcacgtg tatgcgtctg ttcgtagatc atgcccacca
     2221 gaaggaacaa ggcacccgtg cttagtccgt gattgatcat ttgcaggatt gcgcccgcaa
     2281 tgccctgatg attcaaggcg aacaacccaa gcatcacaaa gcccaggtga ctcacggatg
     2341 aatacgcaac cagtttcttg acatcggact gcgcgtacga aaccgccgcg ccgtagataa
     2401 tgccgatcac agcgaagagt gcaatccacg gggcagcttg taaggaggct tgaggaaaga
     2461 gaggcaaatt gaatcgcagg aagccatacg ttcccagttt tagcaaaaca cccgccagga
     2521 taaccgaacc cgctgttggc gcttccacgt gcgcgtcggg cagccaggaa tgcagcggcc
     2581 acatggggac cttgatggca aacgcggcgg tgaatgcaag gaataaccac atttgtgtgc
     2641 ctgccggaat attgccttgc gcggccagat ctggcacaga gaaggtctgc ccctgaatgc
     2701 cgagccacaa gatagccagc agcatcaaaa tagaaccggc catcgtgtag aggaaaaatt
     2761 tgatcgcggc atagatgcgg cgcgggccac cccagatacc gatgatgaag tacatcggca
     2821 cgagtgtaaa ttcccagaag atgtagaaca ggaataaatc ctgcgcaagg aagacgccgt
     2881 tcataccgat ttccagcagg aggaaaaaga tcatgaagtc tttgacgcgt tcttcaatcg
     2941 atgtccatgt cgataagatc gagatcggcg tcagaaacgt ggtgagtaac acaagcagga
     3001 tgctgagtcc atccaccgcc agataatact gaatattcca gcccgccacc tgaatccagt
     3061 tgtaacgcgc ttccagttgc agatccggat ttgatgcgtt gaagcgcgtc aagacccaaa
     3121 tcgaaatgac gagcgtaatc aaactcgtga tgagcgccgt ccaacgcaaa ctattcttat
     3181 tgtcactggg gatgaaaaat aaagccagta cacccagcaa cgggaagaac gtcagtatgg
     3241 taagcggttc aaggaaaaaa ttcatgttct gtcctttccc tcatccccat cccttctcgc
     3301 gaagggagaa gagagccatc ccctctccca atgggagagg gttagggtga gggcgatgtt
     3361 atttcaaaat caaatatcca acaataagta caacgcctaa taagaccgat aaggcatacg
     3421 aacgcacaaa tccattttgg actttgcgca ggactgcgga cgaccattgc gtgactgttg
     3481 cgagtccgtt tgcaaacgca tcaatgccgc gctgatcagc atatttatca agagcaacat
     3541 gactgagcca gttatacgtt cctgcgatga ccttctcatg gaaccagtcg tgccagaaac
     3601 gccaatcgat cacatccgca aggaattgag agatcttcac aaatggcgtg acaatgattg
     3661 cctgatagag ctcatctaca taccatttgt tttccatggc agtgaagatg gaaccaagcg
     3721 atctttttag gggatcgatc tgaccgctct tgagagggtt gcgtccataa atcagccagg
     3781 agaaataaag ggccgccaga gcgaggacag ttgaaatacc agcgaccacg atatcaaggg
     3841 gcaaggcttc cacctcgcct aacgtctgtt cgagccagtg acccagattg tggaatccct
     3901 caaatggaag attcagcgcg ccgcccaaca ccgaaagcac agccaggatc atcaaaggaa
     3961 ccgtcatcac cctggggctt tcctccgcat gcgcagcggc ttcgtgtctc ggctcgccaa
     4021 agaagaccat ccaaatctga cgccccatgt agaacgccgt aaagaacgca gcaatggaaa
     4081 gcaaaatata aacggatcga taatgtaagc tcgcgtcaag gaggatttca tccttggacc
     4141 agaagcctgc aaacgggaaa ataccggcta atgcaagtgt tccaatcatg tagagccaga
     4201 acgtagtagg catggtcttg cgaagcccgc ccatattgcg catatcgccg gggtcaaaga
     4261 cttcaccatg cgcagaagat gaatgtgaat cctgactcgc atgctgtttt tctttgtgtt
     4321 tcttctttcc ctttccctga ccacccccat gatgagcgtg cgcatgttct gcgtggtgat
     4381 gtccacgctc cattcccaaa atgacggagc cagcagaaag gaacaacaac gctttgaaga
     4441 aagcatgtgt gatgagatgg aacatcccag ccacataggc gcccattcca accgcggcaa
     4501 ccatgaagcc taactgtgag atggtggaat aagccaacac tttcttgata tcaaattgac
     4561 cgacagcgat cgtcgcggca aagagcgccg tcacagcacc agtcatcgca acaatgtatt
     4621 gcgcatcgtg aaccgccgaa tagagtggag ccgaacgagt aatcagatat acaccggcgg
     4681 tgaccatggt cgcggcatgg atgagcgcag acaccggggt cggacccgcc atcgcgtctg
     4741 gcaaccagac gtacagcgga atctgcgcgg atttaccggc tacgcctatt aacatgaata
     4801 gcgtgatcgc agtgatcaca ccatgcgaaa cttcatgcac tccttcaaag acagcgtcaa
     4861 attgcaggct gccaaatgcc cagaacatga taaagccagc gatcatgaac ccaaaatcac
     4921 cgacgcggtt tacgatgaac gctttcttgg cggcatttga gttctcccag gaggggcgca
     4981 tcagcagatc catctcgtac cagaaaccga tcagcaggaa ggagcaaagc cccacacctt
     5041 cccatccgac gaacaacatc agataggagt caccgctgac caggatcatc atggcggcga
     5101 tgaacaggtt gaggtacacg aaaaagcgac ggaagcgccc tacatcgttt ttgaaacgca
     5161 catcctcgtg catgtacccg atggcataga tgtgaatcaa agtcccaacc cccgagacca
     5221 ccagcatcat tgtggtggaa agggtatcca cacggaaggt ccaatcgagg ttgagcgtgc
     5281 ccacgtggat ccagtccgcg agataccatc gcagggcttc tccgtgagct gctgaagcag
     5341 tcgaggcaag cagaagggag acaataaacg tcgcaccgga agcaaggctt gcaaccgtgc
     5401 cgatcatccc ttcgctaaat ctgcgaccaa agatgatatt gatcagcaag ccaatgacag
     5461 gcgcgaagac aagccagggt gcaaggtaga ggaaacctgt gtttacatgt tctggcatgc
     5521 ttatcccttc aaactgctca tctggtcaac atcgatactg ttcttggttc tgaaaatttc
     5581 cacgatcaat gcaagcccaa cagcaacctc tgccgcagcg accgcaatga cgaaaaagac
     5641 aaagatctgt ccgctgaagc tttcgagcac gctattgaaa gcgactaagg caagatttgc
     5701 cgcgtttaac atcaactcaa tggacataaa aatgatcaag gcgttgcgtc gaatcagcac
     5761 tcccagcacg ccgatggtga acaggattgc cgaaaggatg atgtaataat tcatggggat
     5821 catgctagcg tacccctttc tcttttttcg taagaatgat cgcgcccacc attgctacca
     5881 ataaaaggat agaaacgatt tcaaacggca ataaataatc actaaagaga gcggtgccca
     5941 actggcgaag attttccatc gtattgacca cagcttcggg ctgcaaaaca tctccggctg
     6001 ggcgagcttt tacaatgagc agatacgcag cttcggcaaa tagaattgct gccagcgtag
     6061 caggaaacca aacgttcctc caattggata tgccaggtaa actctctgcg ccaagcagca
     6121 tgatcacaaa caggaacaaa accatgatcg cacctgcgta cacggtaatc tgcgccatgg
     6181 caatgaaggg tgcgccaagc aggaggtaga aaaccgcgac cgtgacgaag ttcagcacga
     6241 ggaacaatgc cgcgtagacc gcgttgcgac tgagtagcat acccagcgcg ctggcaacag
     6301 cgatcagggc gagaacgagg aacaaaatta ggtcaagagt catggttatt atagaagaga
     6361 ccagaagcta ctctagtctc aaaacactaa tcgtgtggat ctttcatttc cggcacagaa
     6421 cgcctgttgt atttaccggg ttccacttct tgcggagtta gcatggcggt ggtcggcacg
     6481 ggttcgagta acatatcctt cgtgtaaatg gctgcgcgcc ggcttgtata ggacaattca
     6541 taattatcgc ccaggacaat tgcctcggtc gggcaggcat cttcgcaata tccacaaaag
     6601 atgcagcgca gcatgttgat ctcataggtg gaagcatagc gttccccggg agagaaacgc
     6661 ttttcatctg tattctcaga agcttccaca aagatcgcgt ctgaggggca agctgccgca
     6721 cacagtgaac aaccgataca cttttcaagc ccgttatcat aacgtttcag cacatgacga
     6781 ccgcgataac gcgggcgtac gggacgtttc tcctccggat attcatacgt aacaggcttc
     6841 tcaccaatgg acttcagcgt tatccaaagc ccacgcgcca tttcaactat gggatcaagc
     6901 acgcccataa gataatctcc ttatcgaacg ataaatcgta atcaccggga tcaagcccac
     6961 aagtagagaa gcaacgggaa taatccaaat gaacatcggg tcgacttgct ctgccaataa
     7021 aataccggtc gcagtgataa acacaatcgc cagagaaagc ggtatgagga ctttccagcc
     7081 aaacgacatc aaacggtcat agcggatgcg gggccaggtg gcacgcaccc agatcatgcc
     7141 gaaaagcagg ataacaattt tgaggaacat gtacacgggt ccaagcagag ggaaacgatc
     7201 aataccaggg ccaaggtacc cgccaaagaa gaatacagcg aatatcgcac tcacaataat
     7261 catcttttga tattcagcca tgaagaagag tgcaaacttc atgcctgaat attcggagtg
     7321 ataacccgcc gtcagttcct gttccgcttc cggcatatcg aacggtgcac ggttgacctc
     7381 cgccagtgtc acgatcaaaa aaacgagcgc tcccaccggt tgagccacca cgaaccacat
     7441 atccttttgt cgatccacaa tatcatgcaa actcatcgag tccgccagga tgatggcagt
     7501 ca
//
LOCUS       DASTJP010000538        12897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1095797, whole genome shotgun sequence.
ACCESSION   DASTJP010000538 DASTJP010000000
VERSION     DASTJP010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12897
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095797"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFQ23_15755"
     CDS             <1..680
                     /locus_tag="VFQ23_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9908106.1"
                     /translation="YAEYIVVPARNAIPLPKEIRYEEGATLMCASATALHALRKGRVK
                     AGEMVAVFGVGGLGLSAIQLAKALGAIEVFAIDIKQDKLELASEYGAIPIDASRGDPV
                     EVIRKTTKGKGVNVSLEMIGLPGTMMQSIESLGYMGRAVMVGLNQQPIQINTYLQVLG
                     KEAEIIGSNDHLLQELPLLVDMARRKILDTSRVVSQTIPLDATKINQRLDDLENFTSD
                     VRVVIVP"
     gene            695..1189
                     /locus_tag="VFQ23_15760"
     CDS             695..1189
                     /locus_tag="VFQ23_15760"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9908107.1"
                     /translation="MESPKVTRPKFPKGYVDKPTSYLTWEWVAAQLTESKHYWLCSVR
                     PNGHPHVVPRWCVFLDGKIYYDGSPETRHAHNIQTNPNVSLHLESGSEAIILEGIAEP
                     AGKPSNELGKKLAQAYKKYKEFGYSPKPNSWDHGGLFVFTPRQCIAWSNFTQDPTKFV
                     FESE"
     gene            complement(1196..2293)
                     /locus_tag="VFQ23_15765"
     CDS             complement(1196..2293)
                     /locus_tag="VFQ23_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9908108.1"
                     /translation="MRWQWKLGRFAGIDVYVHATFLLLIGWVAYNYWLQYRDWGLVLV
                     GILFIIALFICVILHEYGHALTARKYGIKTRDITLYPIGGVARLERIPDRPIEELWVA
                     LAGPAVNLVISVILIVYLALTNGLPAVANFNLTDGSFIERLIVVNITLVLFNLIPAFP
                     MDGGRVLRALLALRLEYTQATQIAAFIGQGFALLFGLIGLMSNPFLLFVALFVWIGAA
                     QEASMVLMKTSIGGIPVTRAMQTDFQILSPSDTLGRAVELIISGAQQDFPVVENGRVV
                     GVLDRDRLISALSQHGQNALVGEAIRRDVTEIDSHDMVETALMRLQESGAKTLPVTHN
                     GQLVGLITPENITEFLMIRSALKTARKASVP"
     gene            complement(2397..4433)
                     /locus_tag="VFQ23_15770"
     CDS             complement(2397..4433)
                     /locus_tag="VFQ23_15770"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9908109.1"
                     /translation="MHEIPLLINIAIALVVAFCGGLIARRLGLPPIIGYMLGGIVIGP
                     FTPGFVGDTTTISQLAELGVIFLMFGVGLHFSINDFWKVRAVAIPGALGRMVLTTLLG
                     FGLSQLWGWPVSSGIVLGLAISIASTVVLLRGLTDQGLLNTSHGQAAVGWVIVEDLAT
                     VLILVLMPSLVSTQDGFNWSQLALTLLKAAGFVIIVLFVGIRFIPWILLRIAHTRSRE
                     LFILAILAITLGIALGAAELFGVSLALGAFVAGVVVSESPLSHQVAADILPFRDAFAV
                     LFFVSIGMLVNPGYLLDNFGVILGLTALIVIGKALVTFPFGLAFPWPARTTLVLAAGL
                     SQIGEFSFILGQEGVGLGLLDQNQYSLILAGALLSITINPLMFRLITPLEKLLHRFPA
                     VWKIFDRHGVVVPTQIDEEKINGHVVIVGYGRVGSHIADLLSLMNIPYLVVDSVAERV
                     QELDGRGIPTLFGDASNSEVLSHAGLNRARALVVAVPDEAASQLVVASARDFAPALPI
                     LARATTEEGIQQLSDLGAQVVIHPELEGGLEIVRHTLLQLGFPSQEIYRYTDAVRRDH
                     YDLGLNTEEEHRLLNELMDAANRLEVYWFRLNEGNLLVGQTLAQADLRAKTGASVIAI
                     LREGRLIANPKSVTLFQPEDRVGLIGEKEEIEAVEKLLLEPKSTSSEVGFEWET"
     gene            4660..4965
                     /locus_tag="VFQ23_15775"
     CDS             4660..4965
                     /locus_tag="VFQ23_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908110.1"
                     /translation="MITRMWHGRVPTSKAEAYRKFVNERAIPDYQSVEGNISVHILER
                     TEGDITHFITLTFWRNLNSIKAFAGEDLEAAKYYPEDKDFLLEFEPRVVHYEVVGGS"
     gene            4966..5580
                     /locus_tag="VFQ23_15780"
     CDS             4966..5580
                     /locus_tag="VFQ23_15780"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HET9908111.1"
                     /translation="MWFYILQGIGFGFAAAVQPGPFQTYMISQSLTKGWKRSLLIAFS
                     PLISDGPIIALSLLVLNQVPLWLQRFLFVAGGLFVLYLAHGAYQSWKNFDPNKVSDET
                     ADQRSILKGALMIIISPGPYIYWSLVTGPILLKGWREAPILGVGFLAGFYVTVILGLI
                     GLILLFGKARELGQRVNRALIGVSAIALFCFGLYQLWLGVTGNI"
     gene            5663..5959
                     /locus_tag="VFQ23_15785"
     CDS             5663..5959
                     /locus_tag="VFQ23_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908112.1"
                     /translation="MNQPDVLAAVLEAVAEFNEQLEEDHKLELTPETRLLGKSSKLDS
                     FGLVNLIIVVEEKLLDKFDKSITLADDRAMSQEHSPFRSVQSLSDYAYTLLSEN"
     gene            5959..6660
                     /locus_tag="VFQ23_15790"
     CDS             5959..6660
                     /locus_tag="VFQ23_15790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9908113.1"
                     /translation="MSDSVMLITGARKGIGRALAEAFTERGWRVIGCSRQESDYIHKN
                     YRHFVLDVSDQNAVRAMMTSIRKGEGRLDALINNAGVASMNAALLTPIDSVRRIIETN
                     VLGTFLFCQEAARLMQKNKFGRIVNFTSVAVPFNLEGEAIYAASKAAVESLTRILARE
                     LANNGITVNAIGPTPIQTDLLRGVPPEKIQGLLARQAIRRMGEPRDIINAIDFFLRPE
                     SDFITGQVIYLGGVS"
     gene            6735..8090
                     /locus_tag="VFQ23_15795"
     CDS             6735..8090
                     /locus_tag="VFQ23_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HET9908114.1"
                     /translation="MIEFLLDVFHQNKQDDAIIWRNQKFTYEWLLERVRHWSAELFKQ
                     GVAPGTVVILEADFSPNAVALFLALIEHNCILVPLTQSIESKKLEFTQIAEGEIRICI
                     DRNDSVIFEQLPHQAAHELYSILRERQHPGLILFSSGSAGRSKAALHDLAGILEKFKV
                     RRNKQRTITFLLYDHIGGVNTMLYTLSNGGCMITVQDRNPEKVLEAVATYRVELLPTS
                     PTFINMILLSEAYKHHDLSSLKTITYGTEPMPESTLQRFHQLLPNIMLQQTYGLSEVG
                     ILRSKSKSSDSLWVKIGGEGFATRVVDGILQIKADSAMLGYLNAPSPFTEDGWFITGD
                     EVEIDGDYFRILGRRSELINVGGEKVYPAEVESVIQQIENVAEVTVYGEKNPITGNIV
                     CANVLLIQPEDSKVAGLRIKKYCRAHMPTFKVPVKVNIVAEQLHSERFKKMRLSSLHK
                     K"
     gene            8139..8798
                     /locus_tag="VFQ23_15800"
     CDS             8139..8798
                     /locus_tag="VFQ23_15800"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9908115.1"
                     /translation="MNDDFLSYQMTTSSWKYLNGKLKRSIDVMGALIGLVLGFPIFGV
                     ASVIVAIVDKVPVVFTQERIGFKGKPFTIMKLRTLPIVETRDMVSARKIASKPEYRTT
                     YTGKFWRVTSIDEIIQFWLVLKGDMSLIGHRPFPTYYLPFLVDMEGMNKEIVDHYLHV
                     IYQYKPGMSSLSSVCGRGDLTLREKFLYDLVYASQAGFWFDARLIVQTIYIVITCKGA
                     R"
     gene            8800..9642
                     /locus_tag="VFQ23_15805"
     CDS             8800..9642
                     /locus_tag="VFQ23_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908116.1"
                     /translation="MPSVIKATPEQFEQIYPLFSEFHEPRPSREEFQQLFMPRWGSDE
                     SYVGFILVEDNQAVGYLGTLFSVREINGRKEKFCNLCTWIVKEEYRSEGLPLLFQVLR
                     MKDVTVTNFTGNRVAKILEKFDFKVLDKTLRILLPVPTPGDGCELISNKSEILQLLDA
                     QDRRVFDDHSDFKYSFAALKTSEGTSLFCFKKVRRKRLPLLEVHYLSRRDLFFKHIRH
                     TLLSLCLRTGSVGLMIGEHFLSSVSIPFSISIPQRQMRLFRSKTVSAEEMDTMYSELQ
                     ILNL"
     gene            9639..10667
                     /locus_tag="VFQ23_15810"
     CDS             9639..10667
                     /locus_tag="VFQ23_15810"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9908117.1"
                     /translation="MNRWDSLLRIAAPLVRPIYGGAGVILMFHRVLPEAARRRIQGHA
                     ALEVTPRALEDTIQYFARHHYKVYSPDDLYRFLTGTKKADQPFVLFTFDDGYIDNLTY
                     AYPIFKKHNIPFTINISTCFPERTAIIWWYLLEDLILKRERLTFEHEGKYYDFDCMTE
                     EGKAEAYNSTRKLFKFANVAGREQLTKSVLLANGMDLLKKLDEVALTWSQLKQISDDP
                     LVTLGAHTANHYVLSSLSDGEVRFEISESKRILEEKLDTQINHFAYPFGNRREAETRE
                     FRIAAECGFKTAMTTRTGNIYPSHSSHLCALPRHDLAQLSLPRDLNLVTTGALSMRVN
                     RFKRVSIA"
     gene            10746..12008
                     /locus_tag="VFQ23_15815"
     CDS             10746..12008
                     /locus_tag="VFQ23_15815"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET9908118.1"
                     /translation="MKNTNVRFGILLCFVLITACSAGVSSTEVDVPQTESVSTATEKP
                     AVQPPAVDKPFDSTTDFAPANSFALTSTGDTIYMILGREHSLYVSRLSEQESTFTEPV
                     LVTGEQSVHVLRVERPAISANAEGKVGVAWLELDFQGGDNEVWYAQSLDNGQTFEAPR
                     LVGEDPPGESAMVQVLLDPEGNPILTWLTGNKLRFARSSDGGETFSEAKEIGKGACEC
                     CQPAMRQNGNDLLIAYRGMSVDEVGSFRDAALIKSTDGGETFSPVSFATDSHWYIDAC
                     PISGPWITAENDTINMTWMDGRADGPDNLMSSDVWFASSKDNGQTFSSNIKVSGDTST
                     HNFLPVMTVGAGGRIHIVWPAISPDAIYYSISDDNGQTFSIPQVIADDSLHGRPAVPS
                     MVMDAHGRIYLAWLDDEGIRLAVWEDMN"
     gene            12085..12636
                     /locus_tag="VFQ23_15820"
     CDS             12085..12636
                     /locus_tag="VFQ23_15820"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9908119.1"
                     /translation="MPMKLTDPDSEEYAPFYAGYVQRAQARGDVFAALAQQLAEIRTE
                     LGDLSDEQGLFRDAPGEWSIKEVMGHLNDVERVFSYRMLRVSRNDLTPLPGFEQNDYV
                     REAGFDRYTLQDLIQEFEHLRRANILAISNMGEESTLRRGNASGYTVSVRALIYMIVG
                     HVDHHMESLREKYLPPLRQNLNL"
BASE COUNT         3469 a         3177 c         3109 g         3142 t
ORIGIN      
        1 gttatgcaga atatattgtc gttcccgccc gcaacgcgat ccctttacca aaagaaatta
       61 gatacgaaga aggtgcaaca ctgatgtgtg catctgctac agccctgcac gccctgcgca
      121 aaggcagggt caaggctgga gaaatggttg ccgtctttgg cgtcggcgga cttggtttgt
      181 ccgccatcca gctggcgaaa gcactgggtg ccattgaagt gtttgctatc gatattaaac
      241 aggataaact tgaactggct tctgaatatg gcgccatccc aattgacgcg agccgcggtg
      301 atccggtaga agtaatccgc aaaacgacga agggcaaagg ggtgaatgtt tcgctggaga
      361 tgatcggtct gccggggacg atgatgcaat cgatagaaag cctgggatat atggggcgcg
      421 ctgtaatggt ggggttgaat caacagccta ttcaaatcaa cacgtatctc caggtacttg
      481 ggaaggaagc cgagatcatt gggtcaaatg atcatctatt gcaggaactc ccattactgg
      541 tagatatggc tcggcgaaag atactcgata cgtcacgcgt cgtctcacaa accattccat
      601 tggatgcaac taaaatcaat cagcgactgg atgatttgga gaattttaca agcgatgtca
      661 gagttgtgat tgtgccttag gcaaaggata aagtatggaa tctccaaaag tcacccgtcc
      721 taaatttccg aagggatatg tagacaaacc cacttcctac ctgacctggg agtgggtcgc
      781 cgcgcaattg actgaatcca aacattactg gctttgttcc gtccgcccca acgggcatcc
      841 acatgtggtc ccgcgctggt gcgtattttt ggatggaaaa atttattatg atggcagtcc
      901 ggaaacgcgc cacgcgcaca atatccaaac gaatccaaat gtttcgctcc accttgaaag
      961 cggaagcgaa gctatcatct tagagggaat tgccgaacca gccgggaaac cttcaaacga
     1021 attgggaaag aagctcgcac aagcgtataa aaaatataaa gaatttggtt attcacccaa
     1081 accaaactca tgggatcatg gtggattgtt tgtatttacg ccgcggcaat gcattgcgtg
     1141 gtcgaatttt acgcaggacc cgacaaagtt tgtctttgag tctgaataat ttttcctatg
     1201 gaactgaagc cttacgcgct gttttcaaag cagagcgtat cataagaaat tccgtgatgt
     1261 tctctggagt gatcaaacca accaattgac cgttgtgggt gacgggcaag gtctttgcac
     1321 ccgactcctg caaacgcatc agagcagttt caaccatatc atgggagtca atttccgtca
     1381 catcccgacg tatcgcctct ccgacaagcg cgttttggcc atgctgtgaa agggccgaga
     1441 ttaaccggtc gcgatcgaga acacccacca cgcgtccatt ttcgaccact ggaaaatcct
     1501 gttgtgcgcc tgagatgatc aactcaaccg ctcgacccag agtgtctgaa ggggaaagaa
     1561 tttggaaatc cgtctgcatg gcgcgtgtca caggtatgcc accgatagag gtcttcatca
     1621 acaccatgct tgcttcctgg gctgctccaa tccacacgaa caacgccacg aagagcagaa
     1681 acgggttact catcaaaccg atgagcccaa atagcagcgc aaatccctga ccgataaagg
     1741 ctgcaatctg tgtggcctgt gtatattcca atctcagtgc cagcaacgca cgcaggacgc
     1801 gtccaccatc catcggaaag gcggggatca ggttgaataa gaccaacgtg atgtttacaa
     1861 caattaaccg ttcgatgaac gagccatctg taagattgaa atttgcaacg gcgggaagac
     1921 cattggttaa cgcaagatac acaatcagaa ttacactgat cacaagattc accgcggggc
     1981 ctgccagggc aacccaaagc tcctcaatcg ggcgatctgg aatccgttca agtcgcgcta
     2041 caccaccaat cggatacagc gtgatatcac gtgtcttgat gccatacttc cgtgccgtca
     2101 gcgcatgtcc gtattcatgc agtatcacac agataaataa cgcgataatg aacagaatgc
     2161 caaccagaac cagtccccaa tctcggtact gaagccaata gttgtaagct acccatccaa
     2221 tcaacagcag gaaggttgca tgtacataca cgtcgatgcc ggcgaagcgc cccaatttcc
     2281 actgccatct cataaattat ctccttctca agctctccca atttataacg atttttcctg
     2341 acttttatta taaatccagc ggtggtcatt ttttgaccgc gtaaggcggg cgaggcttag
     2401 gtttcccatt cgaaacccac ctcactggat gtggattttg gctccagtaa caatttttcc
     2461 acggcttcga tctcttcctt ttcgccaatc agacccaccc ggtcttcggg ttgaaaaaga
     2521 gtcaccgatt tcgggttggc gatcaaccga ccttcgcgca aaatcgcgat tactgatgca
     2581 cctgtcttgg cgcgcagatc ggcctgtgcc agagtctggc caacgagcag attcccttcg
     2641 ttcagacgaa accaatagac ttcgagtcgg tttgccgcat ccatgagttc atttagcaag
     2701 cggtgctcct cctctgtatt gagcccaaga tcataatggt cgcgccgtac cgcgtcggtg
     2761 tagcgataga tttcctggga cgggaagcca agctgcaaca gagtatgccg cacaatctcc
     2821 agtccgcctt caagttcggg gtggatgaca acctgcgcac ccagatcgga cagctgctgg
     2881 atcccttctt ctgtggtcgc acgggcaagg atgggaagag ccggcgcaaa gtcgcgcgcg
     2941 gaggcgacga ccagctgact cgcggcttca tcgggcacag ccactaccag cgcacgagca
     3001 cgattgagtc cggcatgact taacacctct gagttcgatg catcaccgaa caaagtcggt
     3061 attccgcgac catccaattc ctgaacgcgt tctgcaacag aatccacgac cagataggga
     3121 atattcatca aactgagtaa gtcggcgata tggctcccca ccctgccata tcccacaatg
     3181 accacatgcc cattgatttt ttcctcatca atttgtgttg gcaccactac gccgtgccga
     3241 tcaaatatct tccaaacggc agggaatcgg tgcaacaatt tctcaagagg agtaattagc
     3301 cgaaacataa gtggattaat cgtaatagac agcaacgcac ctgccaggat cagtgaatac
     3361 tgattttgat ccaataagcc aagacccacc ccctcctgac ccagaataaa tgaaaactca
     3421 ccgatctgac ttagaccagc tgccagaacc agcgtggtgc gagccggcca tggaaaggca
     3481 agaccaaatg ggaatgtaac cagcgcctta ccgatcacga tcagcgcagt caaccccaaa
     3541 atgacgccaa aattatccaa caaataacca ggattaacaa gcataccaat tgaaacgaaa
     3601 aagagaacag caaatgcatc gcgaaacggc agaatgtctg cagcaacctg atgactcagc
     3661 ggtgattcgc tgacaacgac tcctgcgaca aacgccccta gcgccagaga aactccaaaa
     3721 agctcagctg cgccgagggc gattcccaat gtaattgcca atattgcaag gataaataat
     3781 tcccgtgatc gagtgtgagc gatacgtaac aatatccacg gaataaatcg gattccgaca
     3841 aaaagaacaa tgatcacaaa tccggccgcc ttaagcaggg tcaatgcaag ttgcgaccag
     3901 ttgaacccgt cttgagtact caccagactg ggcatcagaa ctagaatcag cacagtagcc
     3961 agatcctcta caatcaccca gcccaccgcc gcctgaccat gcgatgtatt taacaatccc
     4021 tgatctgtga ggccgcgcaa caacacaacc gtgctggcaa tcgaaatggc gagccccaac
     4081 acaattccag aggagacagg ccaaccccaa agctggctga ggccaaagcc caacaaagta
     4141 gtgagtacca ttcgacctaa cgcacccggg attgccacgg cacggacttt ccaaaaatcg
     4201 ttgatcgaga aatgcagacc gactccaaac atgagaaaga tcacgcccaa ctccgctaac
     4261 tgactgattg tcgttgtatc gccaacaaat ccgggcgtaa acggaccgat tacgatacca
     4321 ccaagcatat atccaataat tggcggcaag ccaagacgac gggcaatcag acctccacaa
     4381 aacgcaacca caagtgcgat agcgatattt atcaacaatg gaatttcatg catcctcaac
     4441 acctcgcata taagattttg tgagtgtata tcccactgaa tgacggacaa tcatgatggt
     4501 caagcaatct cttgtaaaga ttattaactt tttcggacga tcatgggaat gattacgcca
     4561 agtatacccg acgattctga acatagcttg attgacgcca acccgttaaa atgaagtgga
     4621 cgatcagcaa atgaatccac tttcatcgga gtgctaaaga tgattacaag aatgtggcat
     4681 ggcagagttc caacttcaaa ggcggaggcg tatcggaaat tcgtgaatga acgagccatt
     4741 cccgattatc aatctgtgga aggaaatatc agtgttcata tcctcgagcg gaccgaaggt
     4801 gatattacac actttataac tctcacattt tggagaaacc tgaattcgat caaagccttc
     4861 gctggagaag atttggaggc agcgaaatac tatccggagg acaaagactt tttgctggag
     4921 tttgaacccc gagttgtaca ttacgaagta gtggggggat cctagatgtg gttttacatc
     4981 cttcagggaa tcggcttcgg attcgcggcg gccgtgcaac cgggtccgtt ccaaacgtat
     5041 atgatctccc aatccctcac gaaaggctgg aagcgatctc tgctcattgc cttctcgcct
     5101 ttgattagtg acgggccgat cattgccctt agcctactcg tgctcaacca ggttccttta
     5161 tggcttcaac gatttttatt tgtcgccgga gggctttttg tgctgtacct ggcccatgga
     5221 gcctatcaat cctggaaaaa ctttgaccca aataaagtct ccgatgaaac agcagatcaa
     5281 cgcagcatct taaagggcgc gctgatgatc atcatcagcc cgggacctta tatttattgg
     5341 agtctggtca ctggcccgat tttgctgaag gggtggcgag aagcgcctat cttgggagtg
     5401 ggatttttag caggatttta tgttactgtg attctcggtc tgattggtct cattcttctc
     5461 tttggcaaag cacgggaatt gggacaacga gtgaaccgcg ccctgatcgg cgtctctgcc
     5521 atcgctcttt tttgttttgg cttgtatcag ttgtggctgg gagtcactgg aaacatctaa
     5581 accaggtttt tgcgaaatca cgagatcatc ataacgtgtt cgcttataat tatttagcta
     5641 tgacaaatcg tttggagagt ccatgaatca acccgatgtt ctcgcagccg ttctggaggc
     5701 tgttgctgaa tttaatgaac aacttgagga agaccataaa ctcgaattga cgcctgaaac
     5761 gcgcttgctg ggaaaatctt caaaactaga ctccttcggt ctcgtaaacc tgatcatcgt
     5821 cgttgaagag aagcttctcg ataaatttga caaatcgatt acgctggcag atgaccgcgc
     5881 catgtcgcag gaacacagtc cgtttcgctc cgtgcagtcc ttgtcagatt atgcctacac
     5941 actgttgagt gaaaactgat gtcagactct gtcatgctga taaccggtgc acgcaaaggg
     6001 atcggtcgtg cgttggctga agcatttact gaacgcggct ggcgcgtcat cggttgttcg
     6061 cggcaagagt cagattatat tcacaaaaat tatcgtcact tcgtcctgga tgtcagtgac
     6121 cagaacgcgg tcagagccat gatgacttcc attcgcaaag gcgaaggacg gttggatgca
     6181 ctcatcaaca acgccggggt cgcctccatg aacgcggctt tgcttactcc tattgatagc
     6241 gttcgccgca tcatcgaaac gaatgttctt ggcaccttcc tcttctgtca ggaagccgca
     6301 cggctgatgc aaaaaaataa atttggacgc attgtcaact ttacgagcgt tgctgtcccc
     6361 ttcaatctcg aaggcgaggc tatttacgcc gcatcgaaag ccgcggttga aagccttacc
     6421 cgcattctgg cgcgcgaact ggcgaataac ggcatcacag tcaatgcgat aggacccacg
     6481 cccatccaaa cggacctgct gcgaggggtt ccgcctgaaa aaatccaggg attgctggca
     6541 cgccaagcca ttcgtcgcat gggcgagcct cgggatatta tcaacgccat cgatttcttc
     6601 ctgcgtcccg aaagcgactt catcacgggg caggtgattt atctgggtgg agtttcataa
     6661 ctcacgtaga acgactttcg tcgttcgggg ttgccagtca aaagcgacga aagtttacct
     6721 ctattgtaaa aactatgatt gaatttcttc tcgacgtttt tcaccaaaat aaacaagatg
     6781 atgcgatcat ctggcgaaat caaaagttta cttacgaatg gctgctggag cgcgtccggc
     6841 attggtctgc ggaacttttt aagcaaggtg tcgctcccgg taccgttgtc attcttgaag
     6901 ccgatttctc gccaaacgcg gttgcacttt tcctcgccct cattgagcac aattgcattc
     6961 ttgtgccact gacccaatcc atcgaatcaa agaagctaga gttcacacaa attgccgagg
     7021 gcgaaattcg catttgtatt gatcggaatg attcggtgat ctttgagcag cttccgcatc
     7081 aagcggctca tgagctgtat tccattttgc gagagcgcca acatcccggg ctgattcttt
     7141 tctcgtccgg ttcagcaggc agaagcaaag cggctttaca tgatctggca ggaattttgg
     7201 aaaaattcaa agtgcggcgc aataagcaac gtacgatcac atttctgttg tatgaccata
     7261 taggcggtgt aaacaccatg ttgtacacct tgtccaatgg cggctgtatg atcacagtac
     7321 aggatcgtaa ccccgaaaaa gtgctcgaag cagttgccac ctatcgcgtt gaattgctcc
     7381 ccacctctcc gacctttatc aatatgattc ttttaagcga agcatacaag catcatgacc
     7441 tttcgagcct gaagacgatc acgtacggca cggaacccat gcccgaaagt acgcttcagc
     7501 gattccacca gttgcttccg aatattatgc ttcagcaaac ctatggatta agcgaggtcg
     7561 gaattttgcg ctccaagtca aagagttccg actcattgtg ggtcaaaatc ggaggagagg
     7621 gattcgccac gcgcgtggtg gatgggattc tgcaaatcaa agcagactca gccatgcttg
     7681 gctatttgaa tgcaccgagt ccatttacgg aagatggatg gtttatcacg ggcgatgaag
     7741 tcgaaattga tggggattac ttccgtattc tcggccgtcg ctccgaactg atcaacgtcg
     7801 gtggtgaaaa agtgtatccc gccgaggtgg aatcagtgat ccagcaaatt gaaaatgtcg
     7861 ccgaagtaac tgtctacggt gaaaaaaatc ccattaccgg aaacatcgta tgcgctaacg
     7921 tattgttaat ccaacccgaa gactccaaag tggcaggttt gcgcatcaaa aaatattgcc
     7981 gcgcgcacat gcccaccttc aaagttcctg tcaaagtcaa catcgtggct gaacaattgc
     8041 atagcgagcg attcaagaaa atgcgattga gttcactaca taagaaataa acgataattt
     8101 tgagtcggcg tttgtacgcg ggtccaggga gtgtagctgt gaacgatgac ttcttgagtt
     8161 atcaaatgac aacgagcagt tggaaatact tgaatgggaa attaaaacgg tctatcgatg
     8221 tcatgggtgc gctgattggg ctcgtgctgg gttttccaat ttttggcgtg gcgtctgtga
     8281 ttgtagcgat tgtcgataag gtacccgttg tgttcactca ggaaagaatt ggttttaagg
     8341 ggaaaccctt taccattatg aaattgcgaa ctttgcctat tgtcgagacg cgcgatatgg
     8401 tctctgcaag aaagatagcc agtaaacctg aatataggac cacgtacacc ggaaaattct
     8461 ggagagttac cagtattgat gaaattattc aattttggtt ggtcctgaag ggcgacatga
     8521 gcttaatcgg tcatcgtccg ttccctacgt actatttacc ttttctggtg gatatggaag
     8581 gcatgaataa ggaaatcgta gatcattatt tacacgtaat ttatcaatac aaacctggaa
     8641 tgagcagttt gagctctgtt tgtggaagag gcgacttaac gttgcgtgaa aagtttttat
     8701 acgacttggt ttatgcgagt caggctggtt tttggtttga tgccaggcta atcgttcaaa
     8761 cgatttacat cgttataacc tgcaaagggg cgagataatt tgccatccgt cattaaagcc
     8821 actcccgagc aatttgagca gatttatccc ctctttagcg aattccacga accgcggcca
     8881 tccagggagg aattccaaca attattcatg ccgcgttggg gcagtgacga gtcgtatgtc
     8941 gggttcatcc tagtggagga taatcaggca gtgggatatt taggcacatt gttcagcgta
     9001 cgcgagatca atgggcggaa agaaaagttt tgcaatctct gtacctggat cgtcaaagag
     9061 gaatatcgca gtgaagggtt gccgctgctc tttcaagtgt tgcggatgaa ggatgtaaca
     9121 gtcacgaatt tcacggggaa ccgggtggca aagattttgg aaaaattcga tttcaaggtg
     9181 cttgataaaa cgctcaggat attgttgccc gtcccaacac caggtgatgg ttgtgagtta
     9241 atttcaaaca aatccgagat actccagtta ctggatgcac aggaccgtcg tgtttttgat
     9301 gatcatagcg acttcaaata ttctttcgcc gcgttgaaga cctcagaagg aacctctctc
     9361 ttttgtttca aaaaagtgag gaggaaacgt ctgccgctac tggaagtcca ttatttgagc
     9421 agacgtgacc tgtttttcaa acacattcgc cataccttac tgtcgctctg tctacggact
     9481 ggttcggtgg gcttgatgat cggcgagcat tttttaagta gtgtttcaat ccccttttca
     9541 atcagcattc cccagcgaca aatgcgctta ttccgctcga agacggtctc agcagaagaa
     9601 atggatacca tgtactccga acttcaaatt ctaaacttat gaaccgctgg gattcgttgc
     9661 tccgaattgc cgcaccactc gtccgcccaa tttatggtgg ggctggtgtg attctgatgt
     9721 tccatcgagt cctccccgaa gcggcgcgtc ggcgtattca gggacatgcc gcattggaag
     9781 ttacgcccag ggcgcttgag gacaccatcc aatacttcgc gcgacaccat tacaaagtct
     9841 attcaccaga cgatttgtat cggttcctga ccggaacaaa aaaagccgat cagccttttg
     9901 ttttgttcac attcgatgac gggtatatcg acaatctaac ctacgcttat ccgattttca
     9961 aaaaacataa tatacccttc acgataaaca tcagcacctg tttccccgaa cggaccgcga
    10021 ttatttggtg gtatttgctg gaggatttga ttctcaagcg tgaacgtttg acgtttgagc
    10081 atgaaggcaa gtattacgat ttcgattgca tgacagaaga aggcaaagcc gaggcgtata
    10141 acagcacaag aaaattgttc aagtttgcaa atgttgccgg acgagagcaa ttgacaaagt
    10201 cggttcttct tgcgaacgga atggatctcc ttaaaaaatt ggacgaagtt gctctcacct
    10261 ggagtcaact caagcaaatt tctgacgacc cgctggtgac actcggcgcg catactgcca
    10321 atcattacgt cctgagcagt ttaagcgatg gcgaagtgcg attcgaaatt tcggaatcca
    10381 agcgaatact ggaagaaaaa ctagatacac agatcaatca ttttgcttat ccctttggaa
    10441 accgccgtga agcagaaacg cgtgagttcc gcattgccgc tgaatgtgga ttcaagacgg
    10501 ctatgacaac tcgcaccggg aatatttatc catcccattc atcacacctc tgcgccttgc
    10561 cacgccatga tctggcgcag ctatcgttac cgcgtgactt aaatctggtc accaccggag
    10621 ccttaagtat gcgcgtaaat cgcttcaaac gagtgagtat tgcctagttc attagcaata
    10681 cctgagaggg tgtaaccaga aatcatgctg cactataatt ttcatgttct tgagaggaga
    10741 agatcatgaa aaatacgaat gttcggtttg gaatcctgtt gtgcttcgtg ctcatcacag
    10801 cctgttctgc cggggtgagt tcgacggagg tcgacgtgcc tcaaaccgag tccgtttcaa
    10861 cggcgacaga aaaaccggcc gtccagcccc cagccgtaga taaacccttt gatagtacga
    10921 ctgactttgc tccagcaaat tcttttgccc tgacttccac aggggatacc atctacatga
    10981 tccttggtcg tgagcacagt ctctacgtgt cgcgtttatc cgagcaggaa agtaccttta
    11041 cagagccagt attggttact ggcgaacagt ccgtccatgt tttgcgtgta gaacgcccgg
    11101 caatttctgc aaatgcagaa ggcaaagtag gcgtagcctg gctggaactg gactttcagg
    11161 gaggggataa tgaagtctgg tatgctcagt ccctggataa cggtcaaaca tttgaagctc
    11221 ctcgcttagt cggtgaagat cctcccggag aatctgctat ggtacaggtg ttgctggatc
    11281 ctgaagggaa tccgattctt acctggttaa ccggaaataa acttcgcttc gcccgttcat
    11341 ccgatggagg cgaaacgttc tctgaggcaa aagagattgg caagggcgcc tgcgaatgtt
    11401 gccagcctgc gatgcgccaa aatggaaacg acctgcttat cgcatatcgg ggcatgagcg
    11461 tggatgaggt aggctcgttt cgcgacgccg cgttgatcaa gtccacagat ggcggcgaga
    11521 cgtttagccc agtctccttt gcaactgatt cgcactggta catcgatgcc tgtcccattt
    11581 caggaccatg gatcacggct gaaaacgaca cgatcaacat gacgtggatg gatggtcgtg
    11641 ccgacggacc tgataactta atgagcagtg atgtttggtt tgcgtcatcc aaggataatg
    11701 ggcagacctt ttcttccaac atcaaagtaa gcggagatac aagcactcat aattttcttc
    11761 cagtgatgac ggtcggggca ggagggcgga ttcacattgt gtggcccgcg atttcacctg
    11821 atgcaattta ctattccatc tcagatgata acggacaaac cttcagcatt ccccaggtca
    11881 tcgcggatga cagcctgcat ggcaggcctg cggttccttc gatggttatg gacgcgcatg
    11941 gacgcatcta tctggcatgg ctggacgatg agggaattcg cctcgccgta tgggaagata
    12001 tgaactgatc cgctgaaaac attcagacca cgagcaataa ctgagggttt gatgcgagat
    12061 ataatcggac agaggaaaac ttccatgccg atgaaactca cagatccaga ctctgaagaa
    12121 tacgcgcctt tctacgctgg ttatgtccag cgcgcgcaag ccaggggaga tgtctttgcc
    12181 gctctcgctc aacaactggc cgaaatccgg actgaactag gcgacctgag tgatgaacaa
    12241 gggctcttcc gtgacgcgcc cggagagtgg tccatcaagg aagtaatggg gcatctcaac
    12301 gatgttgaac gcgtgttttc gtaccggatg ctgcgcgtgt cacgaaatga cctcactccc
    12361 ctacccggct tcgaacagaa cgattacgtt cgtgaggcag gctttgatcg ctataccctg
    12421 caggatttga ttcaggagtt cgaacacctg cgacgcgcca atattctcgc catatccaat
    12481 atgggcgagg agtccacgtt acggcgcggc aacgccagcg gctacaccgt cagtgtcagg
    12541 gcattgattt acatgatcgt tggtcatgtg gaccatcata tggaaagcct tcgggaaaaa
    12601 tacctgcctc ccttaagaca gaacctcaat ttataaacgc tcatcgttca aacccgcctc
    12661 acggtatgat tcaacttctg caacccaagt tttcctcctc ccagcacgga tggaagtgat
    12721 ttattgaatc accataaatg ggctccgaac agggccaaga ccgcatataa aaatcatcaa
    12781 ttttgcaacg ccctccgact acgcccggag ggcttgttgt atcctaaagc agtataatcg
    12841 cgtcatatca atttctaacc agagaaaaac atgaaaagcc aggatttacg caccttg
//
LOCUS       DASTJP010000539         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_255011, whole genome shotgun sequence.
ACCESSION   DASTJP010000539 DASTJP010000000
VERSION     DASTJP010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255011"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="VFQ23_15825"
     CDS             complement(<1..1609)
                     /locus_tag="VFQ23_15825"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9908120.1"
                     /translation="MFAKLVTFFHPGRIFHVLVAYRWASLLPALFLLTDNTDSNSPNY
                     LSPLSALIVALLANAAIAVFNRLLNRIVIEYPFAIGLDLLFCAALLAVSGGIHSAYYL
                     YALSPLLAGAFFFQLRGAIAISAAFTPLYLVANSLSLQLNFLVDNISLMTQLAGIWLI
                     PSLFAYPSALLKDINRAREELSKARDELAQKHENLEMAHRQLEIIHDLTMLLQAAPDL
                     LSVQERVLGAVTTDLGFTKAVVALVDPALEELGGWLVYPLKESFPSIPPLPLKPENGA
                     VFKALLDRRVFTTSSEPLVQDAELDSWLHQTEWLVLPLFLREHPVGLLLVDKSSGISK
                     EQESTLMTVANQAALALGTTLLCIDRARRLAIETERNRIARDIHDTVAQSLFGIVYSL
                     DACISMLPLQTNEVRNELIDLRALASSAHEEVRRSIFNLWPSPLTIELFIGDLTNYVN
                     SCCRPRAFSIIFNNRGDFNSLAPGLRRTIYRMAQEALANSARHSDANSARLCLNVTTD
                     QVYLDISDEGRGFDPAIALARNQTREHF"
     gene            1802..2041
                     /locus_tag="VFQ23_15830"
     CDS             1802..2041
                     /locus_tag="VFQ23_15830"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET9908121.1"
                     /translation="MDVIKEDQVLDCSGMLCPMPVVKTSKAIKTMEVGQVLKMVATDP
                     GAPPDMEAWSRQTGNELLRSMQEDGKFIFYIRRKK"
     gene            2061..2588
                     /locus_tag="VFQ23_15835"
     CDS             2061..2588
                     /locus_tag="VFQ23_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HET9908122.1"
                     /translation="MMSKSDQNAPKRLALIASKGTLDWAYPPFILASAAGAMGWDVGI
                     FFTFYGLTLLKPKLTAAVTPVGNPAMPMKMPFGPDGFQNINWPIPQLIMSNVPGFNAL
                     ATTLMKQTFENKGVASIEELRNMCVEMEVRMIGCQMTMDVFGFKREDFIKECEIGGAA
                     TFLEYAADADVQLFV"
BASE COUNT          738 a          608 c          701 g          632 t
ORIGIN      
        1 caaaatgctc acgggtttga ttgcgcgcta acgcgatagc tggatcaaaa ccacgaccct
       61 catcagaaat gtccaggtaa acttgatccg ttgtaacatt caagcacaaa cgggccgagt
      121 tcgcatcgga atgcctggca gaatttgcca aggcttcttg cgccatccga taaatcgttc
      181 gacgtaatcc tggggccagc gaattgaaat cccctctatt attaaagata atgctgaagg
      241 cacgtgggcg gcaacaactg ttcacataat ttgtgaggtc acctatgaaa agttcgattg
      301 tcagcggtga tggccaaagg ttgaagatgg aacggcgcac ttcctcatgt gcgctactgg
      361 ccagcgcacg taagtctatc agctcatttc tgacttcatt tgtctgcaag ggtagcatgc
      421 tgatacacgc gtcgaggctg taaacaatgc cgaataagga ctgtgcaact gtatcgtgaa
      481 tatcccgggc aatccgatta cgttcagttt ctatggcgag tcgtctggca cggtcaatac
      541 ataaaagggt cgtgcccaac gcaagcgcag cctggttcgc aacagtcata agcgttgatt
      601 cttgctcttt gctgattccg ctggatttat ccacgagtaa taatcctacc gggtgttcac
      661 gcaggaaaag tggaagtaca agccattcag tttgatgcaa ccaactatcg agttccgcgt
      721 cttgtacaag aggttcactg ctggtcgtaa aaactctgcg gtcgagcaac gctttgaaaa
      781 ctgcgccatt ttcaggtttc agtgggagtg gaggaatgga aggaaatgat tctttgaggg
      841 gatagacaag ccaaccgccc agttcctcaa gcgccggatc tactaaagcg accaccgctt
      901 ttgtaaagcc aagatcagta gtgaccgcgc caagcacacg ttcctggaca gacagcaggt
      961 caggcgccgc ttggagcagc atagtaaggt catgaatgat ttctagctgc cggtgggcca
     1021 tttcaaggtt ctcatgcttt tgagcaagct catcgcgggc cttcgaaagc tcctcccgag
     1081 cgcggtttat gtctttgagc agagcagatg ggtaggcaaa taggctcggg atcaaccaaa
     1141 tccctgcgag ttgtgtcatc aagctgatgt tgtcgactag aaaattcagc tgcaaactga
     1201 gtgagttggc aactaaatat aaaggcgtga acgccgccga tatcgcaatg gctccgcgca
     1261 gttgaaagaa aaatgctccc gccagtaatg gactgagagc atatagatag taagcgctgt
     1321 gaataccgcc actgactgcc agtagggcgg cacaaaaaag tagatctaac cctattgcaa
     1381 agggatactc gataacaatt ctattaagca gacgattgaa cacagcgatt gctgcgttcg
     1441 ccagaagcgc aacaattagt gccgagagcg gcgaaagata attgggagaa ttactatcag
     1501 tgttgtcggt taataagaac aacgccggta gcaaggatgc ccaccggtaa gccaccaaaa
     1561 cgtgaaaaat ccgaccagga tggaagaatg tcactagctt tgcgaacatg aacgttgtag
     1621 gatgattata tccgaatcac ccgcgcgggg gatgcaaatc cccccgtgtg caggatagtg
     1681 ccaggggaca taccagatta ttatcaacct tagaaatatt gacttggaac caatttaacg
     1741 cagtaacttg gttttcaaat cgccattgaa aaccctagga tccataccac aggaggctct
     1801 aatggatgtg attaaagagg atcaggtact ggattgttca ggcatgctct gtcccatgcc
     1861 tgtagtgaag acctccaaag cgatcaaaac gatggaggtt gggcaggtgc tgaagatggt
     1921 ggctactgat ccaggggctc cacctgatat ggaagcctgg agcagacaaa cggggaatga
     1981 gttgcttcgc tcgatgcagg aagatggtaa gttcattttc tatattcgcc gtaaaaaata
     2041 ggataattta gtaggaggag atgatgtcaa agtctgatca gaacgctccc aagcggctgg
     2101 cactgattgc cagcaaagga actcttgact gggcctatcc cccatttatt ctggccagtg
     2161 cagctggcgc gatgggttgg gatgtgggca ttttctttac gttttatggc ctgacactcc
     2221 taaagccaaa gctgacagca gctgtgacac ccgtgggcaa ccccgccatg ccgatgaaaa
     2281 tgccctttgg tcctgatgga ttccaaaata ttaactggcc gattccgcaa ctcattatgt
     2341 caaacgtccc cgggtttaat gcccttgcta caacgcttat gaagcagacg tttgaaaata
     2401 agggggtcgc ctcgattgag gaattacgca acatgtgcgt agagatggaa gtccgcatga
     2461 ttggctgcca gatgacgatg gatgtctttg gcttcaagcg cgaggatttt attaaggagt
     2521 gtgagatcgg cggtgcagca actttccttg agtacgccgc agatgcggac gtgcagttat
     2581 ttgtttaatg aggtaaccaa atgctttcaa ttgaaacaga aaaccgacaa agcgtatctg
     2641 tggtgaaggt caagggacgc atcgattcgg agactgcac
//
LOCUS       DASTJP010000540         4048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1456680, whole genome shotgun sequence.
ACCESSION   DASTJP010000540 DASTJP010000000
VERSION     DASTJP010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456680"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..946
                     /locus_tag="VFQ23_15840"
     CDS             95..946
                     /locus_tag="VFQ23_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908123.1"
                     /translation="MCTSCNPLDASDRILAALRVIWKEPGSRYSYSEMMGLPKEQLST
                     EYWYPWYNNLDVASMDQGFRIANIDDTPHTVRVFLGTNQVGQDIPLAAGGSTRVGFAI
                     NDGPIKIVCTTCNPANSNDRIITSLRVIWKEPGFRATYSEMMGMPLEQLSTTYWFPWY
                     NNADVASMDQGFRIAIVNGSGENTVQVFVAGALQESITLTENESVRVGYNANNGPAQI
                     VCITCGINEKIVTSLRVIWKEPGVRTSYSEMMGLPDEALSTEYWFPWYNFAAPDSMDQ
                     GFRISVP"
     gene            complement(1018..2202)
                     /gene="metK"
                     /locus_tag="VFQ23_15845"
     CDS             complement(1018..2202)
                     /gene="metK"
                     /locus_tag="VFQ23_15845"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET9908124.1"
                     /translation="MFTSESVTEGHPDKLCDQISDAVLDACLEQDPRSRVACETATKT
                     GFVMLLGEITTTAFVNFDELVRKVVKEIGYDSSDKGFDSHTCAVLTAIASQSPDIAMG
                     VDAALESRNGHEVTDAEVETIGAGDQGMMFGFACNETSTLMPLPIYLAHKLTRRQSEL
                     RKNGTIAWLRPDAKSQVTIEYSQGKPKRIDTVLISTQHAPDVSQAEITETVTEQIIMP
                     ALPEELVDNNIKVYVNPTGRFVVGGPMGDAGVTGRKIIVDTYGGMGRHGGGAFSGKDP
                     TKVDRSAAYAARYVAKNIVAAGLADRVEVQVSYAIGVAHPLSVNVETFGTARIADEKI
                     AALINEFFDLRPGAIIRDLGLRKPIYRKTAAYGHFGRDDIEFPWERTDKAEALKSAAG
                     LS"
     regulatory      complement(2317..2468)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2571..3527)
                     /locus_tag="VFQ23_15850"
     CDS             complement(2571..3527)
                     /locus_tag="VFQ23_15850"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9908125.1"
                     /translation="MKNITALILFFLTACTPINVPTLASTSTPLPTIPTQIVATLTNE
                     IIPTVTVLPTVALTPTAEVVNIKTSSIDGMPQVYIPAGKFHMGGMDVRRAPNEIPDHD
                     VQLSAYWMDQLEVTNAMYGLCMSAGACTAPQSFKSQRRAEYFEIPEFKDYPVVYITWG
                     QARVYCEWAGRRLPTEAEWERAARGDDFRTFPWGENKADGLLTNFNMLVGDTSRVGTY
                     PLGASPFGVLDMAGNVAEWVNDFYTFEYSVSDNLVNPTGPETSSSFNRVVRGGSLGDA
                     EINIRLSKRSSVKGPNPNAVPGSSAYLGDFSPRIGFRCAADE"
     gene            complement(3594..>4048)
                     /locus_tag="VFQ23_15855"
     CDS             complement(3594..>4048)
                     /locus_tag="VFQ23_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908126.1"
                     /translation="IKTLLASNWDATVNRQNALARAEAATVEDPTDAFAWFNVGSNLV
                     YFERYFEANDAYDKARELGLPQRMFRYQFGPFIANFHAQRTEDLLALTDYALQRTDMS
                     EETWLWHGWALYRQGNTNGAVEDWRKALSIHPGYEDALYALNFVGAAP"
BASE COUNT          936 a         1064 c         1050 g          998 t
ORIGIN      
        1 cgtgggaggc aatcaggtgg ggacggatct ggtgctgaat ggtggagcga gtgtgcgggt
       61 ggggtatgct gccaacaacg gaccggcacg ggtggtgtgc acgagttgca atccgctgga
      121 tgccagtgat cggatcctgg ctgcgctgcg ggtgatctgg aaggaaccgg gttctcgtta
      181 tagctactcc gagatgatgg gtctgcccaa agagcaactt tcgaccgagt actggtaccc
      241 ttggtacaac aaccttgatg tcgcttccat ggatcaaggc ttccgcatcg ccaacatcga
      301 tgacacacct cacaccgtgc gtgtgttcct ggggacaaat caggtcgggc aagacatccc
      361 cttggcagcc gggggcagca cgagagtggg ctttgcgatc aacgatggtc cgatcaaaat
      421 cgtctgcacc acctgcaacc cagccaacag caatgaccgc atcatcacct ctctgcgggt
      481 catctggaag gaacctggct tccgtgccac ctactccgag atgatgggca tgccactcga
      541 gcagctctcc accacctact ggttcccctg gtacaacaat gccgatgtgg cttctatgga
      601 ccaagggttc cgcatcgcca tcgtcaacgg cagtggcgag aacaccgtcc aggtgttcgt
      661 cgctggagcc ttgcaggaaa gcatcacgct gacggagaat gagagtgtgc gtgtgggtta
      721 caatgccaac aacggtccgg cacagatcgt ctgcatcacc tgtggcatca acgagaagat
      781 cgtcacctcc ctgcgcgtga tctggaagga gccaggagtc cgcaccagct actccgagat
      841 gatgggtctc cccgacgaag ccctctccac cgagtactgg ttcccctggt acaactttgc
      901 ggctccagac tctatggatc agggttttag aatctcagtg ccgtagtgtt ctttgactat
      961 ttaaaaaaaa gtgcctctta atttcagagg cactttttaa taccaggtta taaattatta
     1021 cgagagtcca gctgcactct tcaaggcttc tgccttatca gtgcgttccc acgggaactc
     1081 gatatcatcg cggccgaaat gaccgtaggc agctgtcttg cgatagatcg gtttgcgcag
     1141 gcccaggtca cggatgattg cgccggggcg caggtcaaaa aattcgttga tgagcgccgc
     1201 gatcttttca tcggcgattc gggctgtgcc gaaagtctcc acattcacgg aaagcggatg
     1261 agcaacacca attgcataag agacctgcac ttccacgcgg tcagccagac ccgcagcgac
     1321 aatattcttt gccacataac gcgcggcata agcggcagaa cgatctacct ttgtgggatc
     1381 ttttccactg aacgcgccgc cgccatgacg acccatcccg ccgtaggtat ccacgatgat
     1441 ctttcgtcct gtgacacctg catcgcccat cggtccacct acgacaaaac gacctgtcgg
     1501 attaacgtac actttgatat tgttatccac cagttcttca gggagtgcag gcatgatgat
     1561 ctgttcggtc accgtttctg taatttcagc ctgtgacaca tcaggcgcgt gttgtgtaga
     1621 aataagaaca gtatctattc gtttgggttt gccctgggaa tattcaatcg tgacctgact
     1681 cttcgcgtca gggcgaagcc aggcaatggt tccgttttta cggagttcgc tttgtcggcg
     1741 tgttagcttg tgtgccagat aaatcggcaa tggcatcagc gtggaagtct cattgcatgc
     1801 aaaaccaaac atcatcccct gatcacccgc accgattgtt tccacttctg catcagtcac
     1861 ttcatgaccg ttacgcgatt ctagtgctgc atccacgccc atggcgatat cggggctttg
     1921 gctggcgatg gctgtgagca ctgcacaggt atggctatcg aagcctttgt cgctgctatc
     1981 gtaaccaatt tccttcacta ccttacgtac gagttcgtcg aagttaacga aggcagttgt
     2041 ggtgatttca cccaataaca tcacgaagcc tgtcttcgtg gcggtttcgc aggcaacgcg
     2101 tgagcgtgga tcctgctcaa gacacgcatc cagcaccgcg tccgagattt gatcacagag
     2161 cttgtcggga tgtccctctg tcaccgattc cgatgtgaac atcagtttgg gcgaggtcat
     2221 aaaagtgttg ctcattctgt tttcctaact cctttgaaaa aaaaattacc ccttcggacg
     2281 tgctgaaagg gcaatctacg ttttggggca gtttccctct catctaccag aacatccttc
     2341 tgtcggaatt ggcaccgtgt tagtgattag ctttttgctt ttagctaaac gctaattgct
     2401 gatcgctaat tgctaatcgg ttgtcgagac atcatcgggc cgggtccctc cgtctctctg
     2461 gataagagcg ccgtattggg gctattgaat tgtggtgcga gtttaccata tgcaaaaaga
     2521 tgagtcaatt aaataatcat tcaatcgtgt tgatgtgaat gttgtcattt ctattcatcc
     2581 gctgcgcaac gaaaaccaat acgcggagaa aaatcaccca ggtaggcgct tgacccgggt
     2641 accgcgttcg ggtttggacc tttcaccgat gaacgcttcg agaggcggat attgatctct
     2701 gcatctccca ggctgccacc gcgtacgact cgattgaagc tggaactcgt ttcgggtccc
     2761 gtcgggttta ccaggttgtc tgaaaccgaa tactcaaacg tataaaaatc attgacccac
     2821 tctgccacat tgcctgccat gtccaacaca ccgaatggac tggctcccaa tggataggtg
     2881 ccgacacgtg acgtatcacc gaccagcata ttgaaattgg tcagtaaccc atctgcctta
     2941 ttttcacccc aaggaaacgt gcggaagtca tcgccgcgcg cggcgcgctc ccattcagct
     3001 tcggttggca gccgcctgcc tgcccattca caatagaccc tggcttgccc ccaggtgatg
     3061 tatacaacag gataatcctt aaactcaggg atctcaaaat attcggcacg tctttgtgac
     3121 ttgaaactct gtggcgcggt acaggcacct gcactcatgc acaggccata catcgcattc
     3181 gtcacttcaa gctgatccat ccaatacgcg ctgagttgaa cgtcatgatc aggaatctca
     3241 ttcggcgcac ggcgcacatc catgccaccc atgtggaatt tgccagcggg gatgtagacc
     3301 tgaggcatgc cgtctatgga ggatgttttt atgttcacta cttcagcagt aggtgtaagg
     3361 gcgactgttg gaagaactgt aacagtagga ataatttcgt tcgttaacgt ggcaacgatt
     3421 tgagttggaa tggttggcag aggcgtagag gttgatgcaa gtgtgggcac atttattggt
     3481 gtgcaagctg tgagaaagaa gaggatcaat gcagtgatgt tcttcatgat gggaattata
     3541 tgtcgttgcg agggccgcgg gcaaaacgtc cggcccaaag caagctcatg tcgttatggt
     3601 gctgcaccca caaagttaag cgcatataag gcgtcttcat atcccgggtg aatcgacaag
     3661 gccttgcgcc aatcttctac tgcgccattc gtattacctt ggcgatataa cgcccagcca
     3721 tgccagagcc atgtctcttc agacatgtcc gttcgctgca atgcgtaatc agtcagtgcc
     3781 agcaaatctt ctgtacgctg ggcatggaaa ttagcgatga acggaccaaa ctgataacgg
     3841 aacatgcgtt gcggcaaacc taattcgcgt gccttgtcat aagcatcatt ggcttcgaag
     3901 tagcgctcaa aataaaccag attgctacca acgttgaacc atgcgaacgc gtccgtgggg
     3961 tcttcaacgg tagcggcttc ggctcgggct aatgcgtttt gccggttgac ggttgcgtcc
     4021 cagttggagg cgagcaaggt tttgatct
//
LOCUS       DASTJP010000541         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_231992, whole genome shotgun sequence.
ACCESSION   DASTJP010000541 DASTJP010000000
VERSION     DASTJP010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231992"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFQ23_15860"
     CDS             <1..426
                     /locus_tag="VFQ23_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908127.1"
                     /translation="YHWTTRDSLINNFIVRADIVLETAHSPASQSGCGFVFNVIDENV
                     HETIFLQRNGSAVYALSGRPFKTEYYDELPNPAEFTMVFLVYENEIHLGINDQEVISY
                     QLELKREKRGWAPALLAGSTQDFGTRCDFKNIELWEITP"
     gene            684..1808
                     /locus_tag="VFQ23_15865"
     CDS             684..1808
                     /locus_tag="VFQ23_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908128.1"
                     /translation="MRFLPFLLILTMVACGPSAQQMTATADAASAQTQTAAPTFTPTL
                     TLTPTMTPTLAASATPSIPKVQGRLQMNFVGLDSNTIIPKPPFGLTLKLTTGDTQIAV
                     EASPEDGSFTTYLEPGTYTISSVSIKNAEFGTEVSELVAYGAQIEIPSQPCYYAGAIM
                     MTAIRLPPGSVDEQLAVVQQLELVGEMQFLFFETGGVLMPVLTESAGAGTCANLPASP
                     EGFQWKYLPESSVAVLAPEQWHFRSEQSETARGYFISLENISTEGSFMTGLTVNVQRD
                     ESRNAMQVAEDLPLKILDLAGIVPPVEVSTRTDGNMTFYEFEYQTAGSRAIIVHNLIA
                     ANQATNTLYVITFESPAEHWEEAWKTGQVMIEQLVFLDNE"
     gene            1965..>2424
                     /locus_tag="VFQ23_15870"
     CDS             1965..>2424
                     /locus_tag="VFQ23_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908129.1"
                     /translation="MNKSISRIVSVLFVLIFMLGLAGPAHAKKSLPVEYAKTDDAPFL
                     LEFTSGGHALGFRSQGMYAATGTHALSVDFVGANPVSPIADTPPSTNGQASVLSKVSY
                     ANLWKGISLEYTATQGGVYMTTYELAPGAHVSDIRLGYNTSLNLDTGGALG"
BASE COUNT          612 a          568 c          605 g          639 t
ORIGIN      
        1 taccactgga caactcgtga cagcttgata aataacttta ttgttcgtgc ggatattgtg
       61 ttggaaaccg cccatagccc tgcttcacag tctggctgtg gttttgtctt taacgtcatt
      121 gatgaaaacg ttcatgaaac catttttctc cagcggaatg gctccgcagt gtacgctctc
      181 agtgggcgtc cttttaaaac ggaatattat gacgaattgc ctaacccggc tgagtttacg
      241 atggtgtttc tcgtatacga aaacgaaatt catcttggca tcaatgacca agaggttatt
      301 tcctatcaac tggaattgaa gagagagaaa cgagggtggg ctccggccct cctggcaggc
      361 agtacgcagg attttggaac acgatgtgat tttaagaata tcgagctctg ggaaattact
      421 ccataactgt atcagagcca agtgtagtat cccaatgcta tttcggatcg tatccaatga
      481 aatgtatgac ttttgcccgc tggcgacacg cagaatgatt acattgtttg tatcatctca
      541 agtgactgga gttcagtttc caatgatgga accccagtat gactcgaagt tggcggtttc
      601 tggcagtcca cgcacttggt ttggcagttt gaaactgtaa aaatggttct agtaattcgt
      661 cattctagat aggatgattt gccatgagat tcttgccgtt tctacttatt ctaacgatgg
      721 tggcttgtgg gccttcggct caacaaatga cggctactgc agatgccgcc agcgcacaga
      781 ctcagaccgc cgcgccaact tttactccca cactcacact gacgcctacc atgaccccca
      841 cactcgccgc tagcgcgacg ccttcgatac cgaaagtgca agggaggctg caaatgaatt
      901 ttgtggggct ggatagtaac accatcattc caaaacctcc gttcgggttg acgctcaagc
      961 tcactacggg tgatacacaa atcgcggtgg aagcctcgcc cgaggatggc agtttcacca
     1021 catatttgga accaggcacc tatacgatct catccgtctc gatcaaaaat gcagagtttg
     1081 gcaccgaggt gagcgaactg gtcgcatatg gtgctcagat cgaaatccct tcacaacctt
     1141 gttactatgc cggtgcaatt atgatgacgg cgattcgatt gcctccgggt agtgttgacg
     1201 aacagttggc tgtggttcaa cagctggaat tggtcggaga aatgcaattc ctcttcttcg
     1261 aaaccggcgg agtgcttatg ccggtattaa ccgagtcagc tggcgctggt acatgtgcaa
     1321 acttacctgc ttccccggaa ggattccaat ggaaatatct gcctgaatca tcggttgctg
     1381 tgcttgctcc cgagcagtgg cattttcgca gcgaacaaag cgaaacagct cgtggctact
     1441 tcatttcttt ggagaatatc agcacggagg gttccttcat gactggtttg acggtcaatg
     1501 tgcaacgcga tgaaagcagg aacgccatgc aagttgcaga agatctacca ctgaagatcc
     1561 tggatctcgc tggcattgta ccgccggtag aggttagcac tcgaaccgat ggaaatatga
     1621 cgttctatga attcgaatac cagactgctg gatctcgggc aattattgtc cacaacttga
     1681 ttgcggcaaa ccaggcaaca aacacactgt atgtcattac atttgaaagc ccggccgagc
     1741 attgggaaga agcctggaaa accggacagg tgatgatcga gcaattggtt ttccttgata
     1801 acgaatagga aaacagaaat gatgttcaat gcgcaggcac gctggcactc aagcattgct
     1861 cgaagttggc ggtttctggc agtccatgca cttggtttgg tagtttgaga atgtcagact
     1921 aagtttgcaa tttgatattc gatcttcagg aggttaccgt gttcatgaat aaatcaattt
     1981 cccgaattgt ttctgtttta ttcgtattga tatttatgct gggattagca ggaccggctc
     2041 acgcgaaaaa gtctttaccg gtagagtatg caaaaactga tgatgcgcca tttttgttgg
     2101 agtttacctc cggtggacat gccctcggct tcagatcaca aggcatgtac gcggctactg
     2161 gcactcacgc tctaagtgtg gattttgtgg gagctaatcc tgtttcaccc atagcggata
     2221 caccccccag cacaaacggt caagcctctg tgctcagcaa ggtgagctat gctaacctgt
     2281 ggaaggggat ctcattggag tacactgcaa cgcagggcgg ggtgtacatg accacatacg
     2341 aacttgcgcc cggtgctcat gtgagtgaca ttcgtttggg ctacaatacc tcgcttaacc
     2401 tcgatacagg cggcgcattg ggca
//
LOCUS       DASTJP010000542         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1394398, whole genome shotgun sequence.
ACCESSION   DASTJP010000542 DASTJP010000000
VERSION     DASTJP010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394398"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFQ23_15875"
     CDS             complement(<1..461)
                     /locus_tag="VFQ23_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908130.1"
                     /translation="MEGRYLIKPSTIQVLLTDDHAIVRKGVRALLATERDIRVIGEAC
                     DGLEAVRQAKALSPDIVLMDLVMPNLDGIEATRQITASLPRTRVLVLTSFAADEKVFP
                     AIKAGALGYLLKDSGPEELIQAIRNVYRGQSSLEPSIARKVLLELSNPPTK"
     gene            complement(486..>2250)
                     /locus_tag="VFQ23_15880"
     CDS             complement(486..>2250)
                     /locus_tag="VFQ23_15880"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9908131.1"
                     /translation="QVTLQSYLAGQSQIYELEHRLRHKDGTYRWILARGSALRGADGK
                     PYRMAGSHTDITERKQAEEKIRQSEKRFWQAFYISPLPIVITTLRDGHYVAVNDAWLC
                     LIGYTRSEVIGTTPDGIRVWARPEESSMVAQELQATGFVHDMERLIQTRAGESRTVLF
                     SAEILDFGDEPHILSFIYDITPLKQTQQELEKRVEERAHELAVLNEISSVVSRSLDIK
                     EILGAALSKAMKMMQMEVGTAYYLEDTEGPDEEKILVLAARYGLSPKFSERVGSRRPR
                     TTALRIAAEAQQPVVWLVANYPDTAVKQALELEGLVQVINIPLSSKDRLVGIFNLGIR
                     YERPIAREELSLLGSIGRQVAVAVDNAHLYDRAEQSAAIAERHRLSRELHDSVTQSLY
                     SVTMYAEAAARLLDTGDIKTATEHLQELRRTAQEALREMRLLIFELRPPILAKIGLAA
                     ALQARLDSVEMRGGMKTHLEVEGGPATKRLPNEVEEELYHIAQEALNNVLKHADAQHV
                     WVQLSFTNGEAVLTVRDDGVGFMPTAPKNGGVGLDSLKERAQKIGATLCVDSTPRAGT
                     TITVNVPVDSSSEGQVGKQHA"
BASE COUNT          490 a          606 c          587 g          567 t
ORIGIN      
        1 tgctttgttg gtggattaga caattcaagc aacactttgc gcgcgatgga tggttctaga
       61 gatgactgac cacgataaac gtttcgaatt gcctggatca gttcttcggg gcctgaatcc
      121 ttcaaaaggt agcccagtgc acctgccttg atcgctggga agaccttctc gtctgccgca
      181 aaactcgtca gcaccaggac gcgcgtgcgc ggtaggctgg cggtgatctg gcgtgtcgcc
      241 tcgatgccat ccaggttggg catgacgagg tccatcagga cgatatcagg ggagagcgct
      301 ttggcctgcc ggaccgcttc caggccatcg caggcctcgc caatcactcg aatgtctcgc
      361 tcggttgcca ggagtgcgcg aacacccttg cggacaattg catggtcatc tgttaagaga
      421 acctggatgg ttgatggttt tataagatac ctgccttcca attatgaacg gaaaaatcac
      481 ataggttaag catgctgttt gccgacttga ccctcgctcg aggaatccac cggaacgttg
      541 acagtgatcg tggtgccggc acggggagta ctgtcaacac acagggttgc cccaatcttt
      601 tgtgctcgtt ccttcaagct atccagacct actccgccat ttttcggcgc ggttggcata
      661 aatcctacac catcgtctcg cacggttaag acagcttcac cattcgtaaa agataactgt
      721 acccatacat gctgagcatc agcgtgcttg agaacgttgt tcagcgcctc ctgggcgatg
      781 tgatagagtt cctcttccac ctcgttgggg aggcgcttcg tcgccggtcc tccctccacc
      841 tccagatgtg ttttcattcc accgcgcatc tcgaccgagt caagacgagc ctgcagagca
      901 gcggcgagcc cgatcttagc caggattggt gggcgtagct caaaaattag cagccgcatt
      961 tcacggagtg cctcctgtgc cgtgcgccgc aactcctgca ggtgctctgt agccgttttg
     1021 atatcgccag tatcgagtaa ccgcgcggct gcctctgcat acatggtcac actgtacagt
     1081 gactgagtaa cggagtcatg taattcgcgt gagaggcggt gacgttcagc gatggcagcg
     1141 gattgttctg ctcggtcata tagatgcgcg ttgtcgactg caaccgcgac ttgtctgcca
     1201 atcgacccga gcaatgataa ctcttcgcgt gcgatgggcc gctcatagcg aattcccagg
     1261 ttgaaaatac caaccagccg atccttagag gatagaggaa tattaataac ctggaccaaa
     1321 ccctcaagtt ccagcgcttg cttcacggca gtatcggggt aattggcaac gagccagacc
     1381 acaggctgtt gtgcttctgc cgctatacgg agtgctgtag tacgcgggcg tcgggagccc
     1441 acgcgctccg aaaatttcgg cgatagccca taccgtgctg cgaggaccag tatcttttcc
     1501 tcatccggtc cttcagtgtc ctccaaatag tatgcagttc ccacttccat ttgcatcatt
     1561 ttcattgctt tgctcaacgc ggcgcccaag atttctttta tgtcaagaga gcggctgaca
     1621 actgatgaaa tctcgttgag gaccgccagt tcgtgagccc gttcttctac gcgtttctcg
     1681 agttcttgct gtgtttgctt gagtggagtg atatcgtaaa taaaagatag tatatgaggc
     1741 tcgtccccaa aatcgagaat ctcagcagaa aacaatacag tccgggattc gcctgctctg
     1801 gtttgaatca gacgctccat atcatgtacg aaacctgttg cctgtaactc ttgagcgacc
     1861 atggaactct cttcaggacg cgcccaaact cggattccgt ctggtgtagt tccgatcacc
     1921 tcgctgcgcg tataaccgat taggcaaagc caggcatcat tgacagcgac gtaatgaccg
     1981 tcacggaggg ttgtaattac aataggaagt gggctaatgt aaaatgcctg ccaaaatcgt
     2041 ttctctgatt gccggatttt ttcttctgcc tgcttgcgct ctgtaatgtc tgtgtgggat
     2101 cccgccatgc gatatggttt gccatcggct ccacgcaggg cactaccgcg agccaaaatc
     2161 caccggtatg tgccatcctt gtgtcgcagt cggtgttcga gctcatagat ttgactttgt
     2221 ccagccaggt aggattggag agttacctgc
//
LOCUS       DASTJP010000543         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_126394, whole genome shotgun sequence.
ACCESSION   DASTJP010000543 DASTJP010000000
VERSION     DASTJP010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126394"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VFQ23_15885"
     CDS             complement(<1..649)
                     /locus_tag="VFQ23_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET9908132.1"
                     /translation="MTQSSNTDSTSTPGPVIMGTWSDEPPLLIPRSAHAVVSTDSAIY
                     ALAGTDDSGKPVLEVEMFDGTHWILETTLPGEGLNASTASIVGSQLFVMGGFTAVSNK
                     PTDEVQVYDLKTQQWSLASPLPNPRGGHVAVVLENRIHLFGGGNSASTLADHSEYDPA
                     TDTWRELAPLPRAEGSPAAVVADGKIYVIGGRSGNSDFGDVYIYDPAADAWANGPS"
     gene            944..1741
                     /locus_tag="VFQ23_15890"
     CDS             944..1741
                     /locus_tag="VFQ23_15890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9908133.1"
                     /translation="MPRQVDLYDSTYGNFQKQVLAEIRSETYGEDIGQNSWITSDEYD
                     RFYSWLNLSAGEHVLEIASGSGGPALYLAKKFKCRITGLDINEEGIKTANQQALDANI
                     AGATFQLADVNQRLPFDDETFDAVMCADSMNHFRDRLGYLKEWQRVLKSGKRALFTDP
                     VVITGPVSNEELAARSNIGFFLFVPLETTEKFIQAAGFKLLRREDVTGNIELTSGRWH
                     AARQKHRDELVKMEGEERFEGLQSFFSTVHKLTSERRLSRFAFLVEK"
     gene            1761..2606
                     /locus_tag="VFQ23_15895"
     CDS             1761..2606
                     /locus_tag="VFQ23_15895"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9908134.1"
                     /translation="MQSVVITGVSTGIGWGIVKVLIEKGFRVFGSVRKTEDAERLSRE
                     FGTNFIPLIFDVTDEQAVQAGAEYVREQLNGETLYGLVNNAGIAVPAPLIHQPTEEFR
                     HQLEVNLVSVLIVTKAFVPLLGSDRSLRGKPGRIINMSSVSGLRGFPFVGAYVTSKHG
                     LEGFSESLRRELILYGIDVIIVGPGPIATPIWDKAEKEDLSKYAHTDYVEAARRAQEY
                     MIRSGRNGYPPEKVGEVVWHALTTPNPRVRYAIVPGNALLRLILPWVPKRVVDRIVAR
                     NLGFK"
     gene            2637..>3232
                     /locus_tag="VFQ23_15900"
     CDS             2637..>3232
                     /locus_tag="VFQ23_15900"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908135.1"
                     /translation="MPAALPRAKVKKNQTWNSESVFASPSAFDAEVDSLIQSLQDLEK
                     FRGHLGENIDTFIDAMDAMNIISQRTAKIQVYGSLSSAVDTSNQQGVSMIIKAMSTVA
                     QVSAATSFVDPELLAIGEAKLRQWLKDDLRMTMYEHYFIDLFRRQAHVRSAEVEQILG
                     MLREPFSNTRGTTNLLANADFKFKPARNSKNKKVELTQ"
BASE COUNT          843 a          745 c          849 g          795 t
ORIGIN      
        1 cggaaggacc gttagcccag gcgtcggcag ctggatcata aatgtaaacg tcaccaaagt
       61 cagaattgcc gcttctacca ccgatcacat agatttttcc atcagccacg accgctgcag
      121 gacttccttc ggcgcgtgga agaggggcta actctctcca tgtatctgtg gctggatcat
      181 attctgaatg atcagcaagt gttgacgcgg aattcccgcc tccgaataaa tgtattcgat
      241 tctctaatac cacggcgaca tgcccaccgc gagggtttgg aagtgggctg gctaagctcc
      301 attgttgtgt cttgaggtca tagacctgga cctcatcggt tggcttattt gacaccgctg
      361 taaagccgcc catgacaaat agctgactgc ccacaatcga tgcagtcgat gcgtttagcc
      421 cttcacccgg caaggtggtt tctagtatcc agtgtgtacc atcgaacatc tctacctcaa
      481 gcacgggctt tccgctatca tcggtgcctg ccagagcata aattgcagaa tctgtgctta
      541 caaccgcatg tgcggaacgt ggaatcaaca agggtggttc gtcactccag gtgcccatta
      601 ttacgggtcc gggggttgaa gttgaatcag tattcgaaga ctgtgtcatt gtcgtagttt
      661 ctgtgagtaa agttgtcggt gtagcaggct gtatggttgg agcaagagtt gatgatggtg
      721 aaatttcgtt tcgattgcca catgcacttt gactaaggag taccccgcaa gtgataagga
      781 tgcttgttcg cgaaatgaaa ttttgagcag tttgcatact ttaaatttat cattgcctct
      841 ataattgggt atccctccaa ggaggggatc aggcaagagc aattcatgtc cgacttgata
      901 taagtaatta agccgatctc aggaaactgt taaggaggtt taagtgccca ggcaggttga
      961 tttatatgac agtacatacg gcaactttca gaagcaggtg ctggcagaaa tcagaagcga
     1021 aacgtatgga gaggatatcg gtcagaacag ttggattacc tctgatgaat atgacaggtt
     1081 ctatagctgg cttaatctct cagctggaga gcacgtcctt gaaatcgcca gcggttcggg
     1141 cggaccagcg ctctaccttg cgaagaagtt caagtgtcga attacgggcc ttgatatcaa
     1201 tgaagagggg atcaaaactg cgaatcaaca ggcgctggat gcaaacattg cgggcgcaac
     1261 attccaactg gcggatgtaa atcaacgcct gccgtttgac gatgaaacct tcgatgcagt
     1321 gatgtgtgcc gactcgatga atcacttccg cgatcggctg ggatacttga aagaatggca
     1381 gcgcgtattg aaatcgggca aacgcgcgtt attcaccgat ccggtcgtga ttacgggtcc
     1441 tgtttcaaac gaagagcttg cggcacgcag caacattggc ttcttccttt tcgtccccct
     1501 tgaaacgact gaaaagttta tacaggcagc aggttttaaa cttctccgcc gtgaggatgt
     1561 aactgggaat atcgagttga cctcgggtcg ctggcatgct gcgcgccaga aacacagaga
     1621 tgagttagta aagatggaag gtgaagaacg gtttgaaggt ttacaatcat ttttctctac
     1681 cgttcataaa ctgacaagcg aacgacgact atcaaggttc gcgttcctgg tggaaaaata
     1741 aacggaggca ggagtatctt atgcaaagcg ttgttattac tggggtttca acagggattg
     1801 gctgggggat cgtaaaagtt ctgatcgaga aagggtttcg ggtatttggc agtgtgcgca
     1861 agacagaaga tgctgaacgg ctttccaggg aattcggaac aaactttatt cctttaattt
     1921 ttgatgttac agatgaacaa gcagttcaag ccggggcaga gtatgtccgt gaacaactca
     1981 acggcgagac cttatatggg ctggtcaaca atgctggcat cgctgtccca gcaccgttga
     2041 tccatcaacc cacggaagag tttcgccatc agctcgaagt gaatctggtc agtgtcttga
     2101 tcgtcacaaa agcgttcgtg cctttacttg gcagtgatcg ctcgctgcgc gggaaacctg
     2161 ggcggatcat caatatgagt tccgtcagcg ggttgagagg ctttcctttt gtcggtgcat
     2221 atgtcacctc aaagcatggg ctggagggat tctccgagag cctgcgacgt gaacttatcc
     2281 tgtatggtat tgatgtgatt atcgtcggac cgggccctat cgcgacgccg atttgggata
     2341 aagcggaaaa ggaagatcta tcaaaatatg ctcataccga ttatgtagag gccgcgcggc
     2401 gcgcacagga atatatgatc cggagcggaa gaaatggcta tccaccagaa aaagtgggtg
     2461 aagttgtttg gcacgcgttg acgacgccga acccgcgtgt tcgctatgcg atagtccccg
     2521 gcaacgccct ccttcgcctc attctgccat gggtgcccaa gcgcgtcgta gatcgaatcg
     2581 tcgctcggaa tttaggcttt aagtaaaacc gccatacact caaaaggaga taaattatgc
     2641 cagctgctct cccgcgcgcc aaggtaaaaa agaaccagac ctggaattcc gaaagtgttt
     2701 ttgcctcgcc ttccgctttc gatgccgaag ttgatagcct cattcaaagc cttcaggacc
     2761 tcgaaaaatt tcgaggtcac ctcggcgaga atattgatac gttcattgac gcgatggatg
     2821 cgatgaacat catctctcaa cgcactgcca aaatccaggt gtatggatct ttatccagcg
     2881 cagtcgatac ctctaatcaa caaggcgtga gcatgatcat taaagccatg agcacggtgg
     2941 cccaagtgag cgctgcaact tcttttgtgg accctgaact cctggcaatc ggtgaagcga
     3001 aattacgtca atggctcaag gacgatttgc gcatgacgat gtatgagcat tatttcattg
     3061 acctgtttcg aagacaggcg cacgtccgca gcgcagaagt ggaacaaatc ctcgggatgt
     3121 tacgtgagcc gttcagcaac acgcgcggta cgacgaattt actggcaaat gctgatttca
     3181 aattcaagcc cgcgcgcaac agcaagaaca aaaaagtgga gctaactcaa ag
//
LOCUS       DASTJP010000544         8457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_739865, whole genome shotgun sequence.
ACCESSION   DASTJP010000544 DASTJP010000000
VERSION     DASTJP010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739865"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFQ23_15905"
     CDS             <1..748
                     /locus_tag="VFQ23_15905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9908136.1"
                     /translation="QLRELDRLKSSFLANMSHELRTPLNSILGFTDVMLEGLDGELTD
                     YMDNDLRLIQKNGQHLLHLINDVLDMAKIESGRMNLHPEKFKVHDVLDEVTSITSTLA
                     SEKNLALFIEEDSNQEIEIFADNTRLRQVMINLVNNSIKFTEKGKIALRVTQVDGARI
                     LITVRDTGMGIPPDHLEDVFQEFTQVDTSTTRKAGGTGLGLPISRRLIEMHGGRLWAE
                     STGVSGQGSTFFVELPIEARITEVIEKQER"
     gene            750..2390
                     /locus_tag="VFQ23_15910"
     CDS             750..2390
                     /locus_tag="VFQ23_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9908137.1"
                     /translation="MSFPKHLECNLCGNQQAYEPFKPAICTKCDSQWLEARYDYDAFK
                     RELLRGLPNRPTNMWRYQDILPLKDPTSLDLYPAGGTPLWLSQRFAPGHGHDSVYIKD
                     ERYGPTSSFKDRQAAGAVAAMLENGIREAVIASTGNAAVAYAAACARAGIKLWVFMTS
                     LVPQEKLREAALFGAEVIRVSGNYDQTKQIAAQFAQRRNLLLDRGANSIPAREAMKTI
                     AYEIVEQLGWHAPDWYIQAVSGGLGPLGVYQGFRELFNMGLINRIPKLAIIQTEGCAP
                     MVNAFKAGKDTAEAVIPETSIIILSTGDPGKAYTYLWNLTQDFGGVMESVTDAEAFAA
                     MRTLAKSEGMAVEPATSVAFAGLEKLIRNGTIHGDEKVVVNCTGHTFPVEKHVLGDQW
                     AVDVHLSKDQSPAPREGLQAALENLDEKTTTVLLIDDNHDDALLVRRLLEGHKSYRVY
                     HAKDGWEGLAMARQKLPDLIVSDLTMPGIDGFGLVEELKLDPRTKDIPVVVVSAKDIT
                     TEERQRLNGHIEGLYQKGSLPPRRFVDQVVQVIEEKSI"
     gene            2405..2782
                     /locus_tag="VFQ23_15915"
     CDS             2405..2782
                     /locus_tag="VFQ23_15915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9908138.1"
                     /translation="MGNSILYIEDNPDNMRLVQRALESRGYRLLQAKNGLDGVQVAER
                     EEIDLILLDINLPDIDGYEVARRLRSSAKQTLTSTPIIAVTANALKGDAERALEAGCD
                     VYMSKPINIRELWARVEAFVPTP"
     gene            2853..4025
                     /locus_tag="VFQ23_15920"
     CDS             2853..4025
                     /locus_tag="VFQ23_15920"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9908139.1"
                     /translation="MNLSFLSHLECSGCGENYNHAEINTFCPNCQSPLLSRYDLDAAR
                     QQVDRDAIKRRKKGMWRWHELLPVLHVENQVFLGEGDTSLLSLPHLEKELNLSHLYVK
                     DESSNPTGSFKARGLAAAVSKAKELGIKKVIIPTAGNAGGAMAAYAARAGLQAHIFMP
                     KDTPIANIEESRMAGAEVTTVDGLISDAAGMAGEKARAEGWFDLSTFKEPYRVEGKKI
                     MGYEIAEAFDWQLPDVIIYPTGGGTGLVGMWKGFDELEALGWLENTKRPRMVSVQSEG
                     CAPVVKAFNKGASFCDFWVNAYTIASGLRVPKSFADHLILEDLYESHGIAIAVSDTAI
                     LESQSQLAAMEGIFAAPEGAATLTALIQLIKQGWIGSDERIVLFNTGSGLKYIDHR"
     gene            4126..5520
                     /locus_tag="VFQ23_15925"
     CDS             4126..5520
                     /locus_tag="VFQ23_15925"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908140.1"
                     /translation="MRYTVLILILAALIISGCGQANLSTPESVTATPVVIPTQTLLPT
                     PTVIPTPVPDTLYVDPSQSLGPISPLVYGSNYGPWIAVSVDMLPAAYDSGVTILRFPA
                     GSWGDQNNVATYQIDQFMAFAGKVGATAMFNVRLLGGTPEQAAEMVRYTNIEKKYGVE
                     YWGIGNEPTLYQSVVKARGETYDTTRFNNEWRTFAEAMKKVDPTIKLVGAEINQFSFD
                     YSPGATTNFSEWDEKWFIDFLKANGDLVDIVSFHRYPFPRSSVSSPVSIEELRQNAQE
                     WDKIVIRARQLIHQYTGRDIPIAVTEFNSDYSKPVGGEASPDSHFNAIWLADVLGRII
                     KNGVFMANHWLLTSKGGLGGWGLVGPSEPYPSYYVYQMYKKFGNELVYSSSDDSNLSV
                     YAARREDGTLTILVINLASEEQTKAIRIEGQAEVKAEAWLFDRTHKAETIGIVSFADP
                     IRFRAQSITLFINP"
     gene            5637..6659
                     /locus_tag="VFQ23_15930"
     CDS             5637..6659
                     /locus_tag="VFQ23_15930"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9908141.1"
                     /translation="MRRRSSSVTIRDVARKADVSVATVSRYINRNAPVSPEVAERLDQ
                     VMTEMRYVPHAAARHLASRKTHVIGLLLNNLHNDFFVPLLNGVEGVIRNRGYNLIIAT
                     YHASSRNGTPPPIGPHNTDGLLVFSDGLVDDDLINLNGREFPMVLVHRTSPASVSIPS
                     VNVENTAITQKLVEHLIQVHGKRRIMFLRGPASQEDSMRREAGYKSALQANGIPFDEN
                     LVLAGEFERDIAYQAMDEFLGNGKRVAFDAVFTGDDDAAIGVLRALHQHQIRVPEDVA
                     VTGFDDLGFAPFLNPPLTTVRAPTEDVGRIATERLFGLLENGPSDEVLILPTEIIYRR
                     SCGCQS"
     gene            6763..8244
                     /locus_tag="VFQ23_15935"
     CDS             6763..8244
                     /locus_tag="VFQ23_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9908142.1"
                     /translation="MKKLLYVLSLLVIASMVLTACGGAPAATEPPAATEPAATEPPAA
                     TEPPAATEPAASGDRVQIRWFVGLGTGTNPEQVAVQEEVVEDFNASQDAIELVLEIVP
                     FDAARDTLSTQIASGAGPDIVGPVGWGGSNDFYGQWLDITPYIESSGFDTSIFDPALV
                     EFYQTEEGQVGLPFAVFPGAFYFRPDMFDEAGLNYPPQVYGEKYVMPDGSEVDWNWET
                     VTEVAKLLTVDVNGLNSTEDGFDNTQIVQVGYEPQWQSILSVGTFYGGAAPIYSGDTK
                     GSYESTIPDSWKEGWQWLYDGMYGEQPFIATGPLNGAPEFGNGNTFNAGKAAMGLTQT
                     WYTCCLADFAAAGNEFQLGIQPIGANGEVSGRIDADTFRILKTTEHPEEAFEVLAYLI
                     TTGADKLLPIYGAMPAVPSKTEAFFEAKSADYPFVTQESWDVFVQGLAYPDTPSSEQY
                     QPNSVKANARFATFFDLLRNTEGLDFDAEYQKLIDDLNVLYNE"
BASE COUNT         2162 a         2015 c         2158 g         2122 t
ORIGIN      
        1 tcaattgcgt gagctcgacc gcttaaaatc gtccttcctt gcaaatatgt ctcatgaact
       61 gcgtacaccg ttgaactcaa tcctcggctt taccgatgtg atgttggaag gtctggatgg
      121 tgaactcacc gattacatgg ataatgatct tcgtctgatt caaaagaatg gtcaacatct
      181 cttgcatttg atcaacgatg tgctggatat ggcgaagatc gaatctggtc gcatgaattt
      241 acatcctgaa aaattcaagg tacacgatgt tttggatgag gttactagca tcacctcgac
      301 tttggcaagt gaaaagaatc tggcactctt cattgaagag gattccaatc aggaaatcga
      361 aatctttgca gataacactc gtttacgcca agtgatgata aacctggtaa acaactccat
      421 aaagtttaca gaaaaaggca agatcgcttt acgtgttaca caagtagacg gtgcaagaat
      481 cttgatcact gtcagagaca ccggtatggg tattcctcca gaccacttgg aagatgtctt
      541 ccaggagttt acccaggtgg atacatctac cactcgcaaa gcaggtggta ccggtttggg
      601 cttgccgatc agtcgacggc tcatcgagat gcacggcggc cgcttgtggg ccgaaagtac
      661 gggagtttct ggtcagggtt caaccttctt tgtcgaattg ccgattgagg caagaattac
      721 tgaagttatc gagaagcagg aaagataaga tgtcattccc aaaacatctt gaatgtaact
      781 tgtgcggcaa ccaacaagct tacgagccgt tcaaacctgc gatttgtaca aaatgcgatt
      841 cgcaatggct ggaggcgcgc tatgattatg atgcattcaa gcgagaactg ctacgcgggc
      901 ttcccaatcg cccaaccaac atgtggcgct atcaggacat cctacccctc aaagacccca
      961 cctccctgga tctttaccct gctggtggaa ctcctctctg gctttcacaa cgcttcgccc
     1021 ccgggcatgg acatgattcc gtttacataa aagatgagcg ctatggaccg acctcctctt
     1081 tcaaagaccg acaggcagct ggggcagtgg ccgccatgct ggaaaacggg attagggaag
     1141 cagttatagc atccactggc aatgcggctg tagcatacgc tgcagcctgc gcgcgagccg
     1201 ggatcaagct ttgggtcttt atgaccagcc tggttccgca ggaaaaattg cgtgaagccg
     1261 cactgtttgg tgcggaagtg atacgcgtga gcggcaatta cgatcagaca aagcaaattg
     1321 ctgctcagtt cgcacaacgc cgcaacttgc tgcttgatcg aggtgccaat tcaattcctg
     1381 cccgcgaggc aatgaagaca attgcctatg aaatcgtgga acaacttggc tggcatgccc
     1441 cggactggta tattcaagcg gtcagcggtg gtttgggacc attaggtgtc tatcaggggt
     1501 tcagagaatt gtttaatatg ggcttgatca accgcatccc caagctggcg atcatccaga
     1561 cagaaggctg tgcgccaatg gtgaatgcgt tcaaagccgg caaagatacc gcggaagccg
     1621 tgatccctga aacaagcatc attattttat ccactggtga cccgggcaag gcctatacct
     1681 acctttggaa tctgacgcag gattttggtg gtgtaatgga atcggtgacc gatgctgaag
     1741 catttgcagc catgcggacg ctcgcgaaaa gcgaaggaat ggctgttgaa cctgctacct
     1801 ctgtcgcctt tgcaggactt gaaaagttga ttcgcaacgg aactatacat ggggatgaaa
     1861 aggttgtggt aaattgtaca ggtcacacct tcccggtcga aaagcacgta cttggcgatc
     1921 aatgggcagt ggatgtgcac ttgagcaagg accaatcgcc tgcgccgcgt gaaggcttgc
     1981 aagctgctct cgaaaatctt gatgagaaga caacgaccgt attattgatc gatgataacc
     2041 acgatgatgc actactcgtt cgtcgattgt tggaaggtca caagtcttat cgtgtgtacc
     2101 atgcaaaaga tggatgggaa ggcctggcaa tggctcggca gaaattacct gatttgattg
     2161 tctccgatct caccatgccc ggcattgacg gtttcgggct ggtggaagaa ctcaagcttg
     2221 acccacgcac aaaagatatc ccagtggtcg tcgtcagtgc gaaggatatc acgacggaag
     2281 aacggcaaag actcaatggg catatcgagg gtctctatca aaaaggttca ctacctccac
     2341 gcaggtttgt ggaccaggtt gtgcaagtca tcgaagagaa aagcatttaa caaggagtgt
     2401 gactatgggc aatagcattc tttatatcga agataaccct gataacatgc gactggttca
     2461 gcgagctttg gagtcacgcg gctaccgtct gctccaggcg aagaacggac tggatggggt
     2521 tcaagtcgcg gaacgcgaag agattgacct gatcctgtta gatattaacc tgccagatat
     2581 cgatgggtat gaagtggctc gtcggcttcg cagcagtgcc aagcagaccc ttacttccac
     2641 tccgattatt gcagtgacag ctaatgcatt gaagggcgat gccgaaagag ctctggaggc
     2701 tgggtgcgat gtatatatgt ccaagccgat caatattcgc gagctctggg cacgagttga
     2761 agctttcgtt cccacaccct aaatttgaac gtgataaact ccaatggcaa taacgggcgt
     2821 cgcctcggca tgcaacgtta tttgccaatg cgatgaattt atcctttcta tctcatcttg
     2881 aatgttcagg ctgcggcgag aattataacc acgctgaaat caatacattt tgtccgaact
     2941 gtcaatcacc tctcctttcc cgatatgatc ttgatgcagc gcgccagcaa gtagatcgcg
     3001 atgcaatcaa gcgccgaaaa aaggggatgt ggcgctggca tgagttactc cccgtcttac
     3061 atgtggaaaa tcaggtattt ctaggtgaag gggatacatc tctactctcg ctgcctcatc
     3121 ttgaaaaaga attgaatctt tctcatcttt atgtgaaaga tgaaagcagt aacccaaccg
     3181 gttcattcaa ggcgcgcggt ctggcggccg ccgtctccaa agccaaagag cttggcatta
     3241 aaaaagtaat catccctaca gccggcaacg ctggcggggc aatggcggct tatgccgcac
     3301 gcgcggggct gcaagcccat atttttatgc caaaggacac gccgattgct aatattgaag
     3361 aaagccgcat ggcgggcgcg gaggtcacaa cggtggacgg cttaatcagc gacgcggctg
     3421 gcatggcggg cgaaaaagca cgtgcagaag gctggtttga tctttcaact tttaaagagc
     3481 cctatcgtgt ggagggcaaa aagatcatgg ggtatgaaat cgctgaagcc ttcgactggc
     3541 agttgccgga tgtgattatc tatccgaccg gcggaggcac cggactggtg ggcatgtgga
     3601 aaggatttga tgaacttgaa gcattaggct ggctggaaaa tacaaaacgt ccgcgcatgg
     3661 tctctgtaca gtcggaggga tgtgcaccag tggtgaaggc gtttaacaag ggagcttcct
     3721 tttgcgattt ctgggtgaat gcttacacaa tcgcttctgg cttacgcgtt ccgaaaagtt
     3781 tcgccgatca tcttatcctt gaggatctgt atgaaagcca tggcatagct atcgcggtaa
     3841 gcgacacggc catcctggag tcacaaagtc aattggcagc tatggaagga atattcgcgg
     3901 cacccgaggg cgccgcgact cttacagcgt taatccagct gattaagcaa ggttggatcg
     3961 gctctgatga acgcatcgtg ctatttaaca ccggttcggg cttaaaatac atcgatcaca
     4021 gatgaggcgc aataattgat agtgggcatc ttcaaaggag atgccctttt tatttcgtgg
     4081 acttggtagt ataaacagcg actaaattat attttggaga ctgacatgcg ttatacagtg
     4141 ttgattttga ttcttgccgc tctgatcatt tccggctgcg ggcaagccaa tctttctacg
     4201 cctgagtctg ttaccgcaac tcccgtcgtt atccctactc agacgttact accaactcca
     4261 actgtcatcc cgactccagt tcccgataca ttatatgtag atccatcgca gtcgcttgga
     4321 ccgatcagtc cgctggtcta tggatcaaac tacggaccct ggatcgctgt atctgtagat
     4381 atgctgcctg ctgcttatga ttcgggcgtg acaattttgc gcttcccggc aggctcctgg
     4441 ggagatcaaa acaatgtagc cacctatcaa atcgatcaat ttatggcatt tgcgggcaaa
     4501 gtcggcgcca cagctatgtt caacgtccgg ttgttgggcg ggacacctga acaagctgca
     4561 gagatggtgc gctatacgaa tattgaaaag aaatacggtg tagagtattg gggcattgga
     4621 aatgaaccaa cgctttacca gagtgtagtg aaagccagag gtgagacgta cgatacaaca
     4681 cgtttcaata acgaatggcg gacttttgcc gaagccatga aaaaagtgga tccgaccata
     4741 aagcttgtag gagcagagat caatcaattc agttttgatt attcgcctgg tgcaactaca
     4801 aatttcagcg aatgggatga aaaatggttt atagacttcc tgaaggcaaa tggggatctt
     4861 gtcgatattg tttctttcca ccgttatcca tttccccgtt caagtgtatc aagtcccgtt
     4921 tctattgagg aactgcgtca aaatgcccaa gaatgggaca agatcgttat ccgcgcgcgt
     4981 caactcatcc atcaatatac cgggcgggat atacctattg cggttactga attcaattcc
     5041 gattatagca agccagtagg cggtgaagct tcgcctgatt cacacttcaa tgcgatttgg
     5101 ctggcagatg tgttggggcg gatcatcaaa aatggtgtat ttatggcaaa ccactggctt
     5161 ttgacctcaa aaggcgggtt ggggggctgg gggttagttg ggccgagtga gccgtatcca
     5221 agttattatg tttaccaaat gtacaagaaa tttggaaatg agcttgtata ttcatcttcc
     5281 gatgattcga atctttcggt ttacgccgcg cggcgcgaag atggcactct cacgattctc
     5341 gttatcaatt tggcgtctga agagcagacc aaagctattc gaatcgaggg tcaggcggag
     5401 gtcaaggcgg aggcgtggct gtttgatcgc actcacaagg cagaaaccat aggaatagtc
     5461 agttttgccg accccatcag gttccgtgct cagtcaatta cgctgtttat caatccataa
     5521 gcctttaaaa tacgtatatt ttccaagaac gattttttaa ggtaagcgtt gcttgacttt
     5581 gattttggga tatgaaataa tgtaaccgtt tacagtaacc gtttacatta acccaaatga
     5641 gacgacgctc ttcttccgtg acgatccgtg atgtagcccg aaaggctgat gtttccgttg
     5701 ccacagtaag ccggtatatc aatcggaatg ccccagtatc acccgaggtt gcagagcggc
     5761 tggaccaggt catgacagag atgaggtatg tccctcatgc cgccgcccgt catctggcaa
     5821 gtcgcaaaac ccacgtaatc ggtcttttat tgaataattt gcataatgat ttctttgttc
     5881 ccctcctgaa cggcgtggaa ggggtaattc gcaaccgggg gtataactta attattgcta
     5941 cgtatcacgc aagcagccga aatggaactc ccccgccgat tggtccacat aatactgatg
     6001 gtttactggt gttttcggat ggtctggttg atgacgatct gatcaaccta aatggcaggg
     6061 agtttccgat ggttttggtg catcgaactt ctcctgcttc tgtctccatt ccaagtgtga
     6121 atgttgaaaa tacggcaatt acacaaaaat tggttgaaca cctcattcag gttcacggaa
     6181 aacgccgcat tatgttcctg agaggaccag ccagtcagga agattccatg cggcgtgagg
     6241 cgggttataa gtcggcgttg caggcaaatg gaatcccttt tgacgaaaat ctggttctcg
     6301 ctggtgaatt cgagcgcgac attgcttatc aggctatgga tgaatttctt ggaaatggta
     6361 agcgggtcgc gttcgatgct gtatttacag gggatgacga tgcagcaatt ggtgtgctgc
     6421 gggctctaca tcagcatcaa atccgagtgc cagaagacgt ggctgtgacc gggtttgatg
     6481 atcttggttt tgcgccattt ctaaacccac ctttgacaac ggtgcgggcg cccacggaag
     6541 acgtggggcg gattgcgacg gaaagattgt ttggtctgtt agagaacggt ccctcggatg
     6601 aggttctaat tcttccgaca gagattattt accgccgttc ttgtggttgc cagtcatgaa
     6661 aaaaaacctt tcgatgaagg aggtgagtgc tttcaccaat acccattcca ttttccaaat
     6721 ttttgtcaaa tccaaatact agttatgagg agaaaactca ccatgaagaa acttttgtac
     6781 gttctgtccc tcctggtcat cgcctcgatg gtactgaccg cctgcggtgg tgcacctgcc
     6841 gctacagaac cacccgccgc gaccgaacct gcggctaccg aaccgcctgc cgctacagaa
     6901 ccacccgccg caaccgaacc ggcagcctcc ggagatagag tccagattcg ttggtttgtc
     6961 ggtctcggaa ccggaacgaa ccctgaacag gttgctgttc aagaggaagt cgtcgaggat
     7021 tttaacgcct cccaggatgc cattgaactc gtcctcgaaa tcgtaccctt cgatgctgca
     7081 cgcgacactc tctccacgca gattgcctct ggcgccggac ccgacatcgt tggtccagtc
     7141 ggttggggtg gttccaatga cttctatggc cagtggttag atattacacc ctacattgag
     7201 tcaagtggtt tcgataccag catctttgac ccggctttgg ttgagttcta tcagaccgaa
     7261 gaaggacaag ttggtttacc ctttgctgta ttccccggtg cattctactt ccgccccgat
     7321 atgttcgatg aggccggtct aaactacccc ccacaagttt atggcgagaa atacgtcatg
     7381 cctgatggtt ccgaagtgga ctggaactgg gaaactgtta ccgaagttgc caaacttctg
     7441 acggtagatg tgaatggtct gaattccact gaagacggtt ttgacaacac acagatcgtt
     7501 caggtcggtt acgaacctca gtggcaatcg atcctttctg tgggtacatt ctacggtggc
     7561 gcagcgccga tttactccgg cgatacgaaa ggctcctatg agtccaccat tcccgatagc
     7621 tggaaagaag gctggcaatg gctgtacgat ggtatgtatg gcgaacagcc cttcattgca
     7681 actggtccat tgaacggcgc gcccgagttt ggcaacggca acaccttcaa cgccggcaaa
     7741 gccgccatgg gcttaaccca aacctggtac acctgctgtt tggctgattt cgcagcggct
     7801 ggcaatgaat tccagctcgg cattcaacca atcggtgcaa acggcgaagt gagtggccgc
     7861 attgatgctg ataccttccg cattctcaag accaccgagc atccagaaga agcctttgaa
     7921 gtgcttgcct atcttatcac cacaggtgcc gacaaactcc tgccgatcta tggtgctatg
     7981 cccgctgtgc cgtcaaaaac cgaagccttc ttcgaagcaa aatccgcgga ttatccattt
     8041 gtgacacagg aaagctggga tgtctttgtc cagggcttag cctatccaga taccccaagc
     8101 tcagaacaat accagcccaa ttccgtcaaa gccaatgcac gcttcgcaac cttctttgac
     8161 ctgttgagaa acactgaggg tctggacttt gatgctgaat atcaaaagtt gatcgacgac
     8221 cttaacgtct tgtacaacga gtaagcacaa gttaaaatgc acattgggcg ggttggtcac
     8281 aaaactgatc cgcccaatgt gttcttagct tcttactagt caaaaggagt gcaccatgct
     8341 tgagcaactt cagaaattac ccaaggtatc cacgttgact tcggtacgaa aaatgatgcc
     8401 gctgagccgt cgcgaaatga gaaacggtct tttgtttctc tctccctggc tcttggg
//
LOCUS       DASTJP010000545         6201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_58725, whole genome shotgun sequence.
ACCESSION   DASTJP010000545 DASTJP010000000
VERSION     DASTJP010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58725"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFQ23_15940"
     CDS             complement(<1..678)
                     /locus_tag="VFQ23_15940"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9908143.1"
                     /translation="MAEKSQTHDKQVLHKLGYAQELARRMSGFSNFAISFSIICILAG
                     GISAFPAAFNVLGSGGAFVIWLVGGILALSVAFGLGQIASSFPTAGGLYHWSSHLGGK
                     AWGWATAWFNLVGLICVVSSVDVLLYTVFFKGILLTDTLGMDTSSFGYWHQFFFVAVV
                     LISQALLNHYGIDITTKVTDLSGYLIFGLTIILIIALFVFNPVAVDFSRLFRFENFTG
                     DAGGGVVP"
     gene            complement(864..1337)
                     /locus_tag="VFQ23_15945"
     CDS             complement(864..1337)
                     /locus_tag="VFQ23_15945"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9908144.1"
                     /translation="MTTKPNKARANFKLPPPLLGLVHIVIAVVLARLVPLPWVAPPFL
                     QTLGFLLVIVGFLLGIGAAMAFRRARTTMNDQGSTARLVTFGIYRFTRNPIYLGFLFM
                     LIGIPLNAGSYWGLLLTPFMIFMFDKFVIIPEEKYLFKRFGEEYSTYQSRVRRWV"
     gene            complement(1599..3401)
                     /gene="pabB"
                     /locus_tag="VFQ23_15950"
     CDS             complement(1599..3401)
                     /gene="pabB"
                     /locus_tag="VFQ23_15950"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET9908145.1"
                     /translation="MTRANKPQSFVRTNEVLLKAESTWLHFANPHRVILAQKQDEVIP
                     ALLEIERLTQDNDWHAAGFVSYEAASAFDLALRTRASDAPPDENLPLRPIDGDFPLLW
                     FGLYSAPEWISEIGDPHDNSTSRLNWKPTIDRETYNKAIEQIRSHIADGRTYQVNYTM
                     RLQTDFTGSEWDFFLHLTQNQNKHAAYIDIGRYVICSASPELFFQLDGDRIICQPMKG
                     TVKRGRTTLEDELQSQWLKSSEKNRAENVMIVDMIRNDVGRIAKLGSVEVPHLFDTER
                     YSTLWQMTSTVTAKTSESLTEIFTALFPCASITGAPKVRTMKIITALESTPRKIYTGS
                     IGYVAPNRKASFNVAIRTGLIDRESKQAEYGVGGGIVWDSTSGDEYEEALLKARVLTE
                     QTPQFSLLETLLWTPDHGFFLRKKHVERMLDSANYFNFPILEKDIDRYLDKLSSTFDS
                     PQRVRLLLHKTGELNSEAKSFHPVQHTSPLKVKLAREPIETDNIFLFHKTTHRKVYDM
                     ARQGCTDCDDVLLYNDRGELTEFTIGNLVVEFEGKLYTPPLSSGLLAGTFRSHLLDIG
                     QISERSILAEELKVFTRIFMINSVRKFQGVGVDV"
     gene            3505..3966
                     /locus_tag="VFQ23_15955"
     CDS             3505..3966
                     /locus_tag="VFQ23_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908146.1"
                     /translation="MKNFRILFAAAILVLVSLACQRLSGGSGGGGNEPLSPTSVGDSS
                     TPAVAPTEPSGNGNTSSTGFPMTADAYDVTEIGDGSLLYYTNLSLEEVMTFYRAEYTS
                     QGYTEREILTVVSDGVFSMVFDGDPSGKGVVIQSVDLGDGSRTVAIRLEDV"
     gene            4025..4417
                     /locus_tag="VFQ23_15960"
     CDS             4025..4417
                     /locus_tag="VFQ23_15960"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET9908147.1"
                     /translation="MRKFSFELRKWLPSLLMMGLIFWFSAQPSYNLPDFNWADRIVKK
                     GGHILGYGLLAWGNWSALEGKPNRHWLAWLLAILYAVTDEFHQSFVVGRFPSVWDVVV
                     FDNLGAVISIWLLRQINKNAMPKSAQII"
     gene            complement(4448..5218)
                     /locus_tag="VFQ23_15965"
     CDS             complement(4448..5218)
                     /locus_tag="VFQ23_15965"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET9908148.1"
                     /translation="MDNLLGRQLHYNVQTPVYEGPLDLLLNLIERAELDITSVSLALV
                     TDQYLSYIKGLEQINADEISAFIVIAAKLLQIKSEALLPRPPAPTPGEEDTGRSLVDQ
                     LKLYKRFKEIGGWLNNQQNANLRTYLRIAPPPKVEPKLDLSNLTLERLVTAAEVAFAK
                     EKEKQPLGVIIAPPRITIREKIDMIAKTLKEVERSTFSALLNANASRLEVVVTFLALL
                     ELIKRYRVAAHQEGLFDDIAIDRMDTWEDDDEMELEFE"
     gene            5276..5755
                     /gene="moaC"
                     /locus_tag="VFQ23_15970"
     CDS             5276..5755
                     /gene="moaC"
                     /locus_tag="VFQ23_15970"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET9908149.1"
                     /translation="MQNKLTHLDDQGRAKMVDVGHKPDTERIAVARCEVHMLKETLEL
                     IRAGQIKKGDVLTIAQIAGITASKRTSDLIPLCHPLPLTKVDVDLELDDSIPGVVITA
                     TAKTIGKTGVEMEALTAVSISALTVYDMAKAAEKTMRIQNIRLVEKHGGQSGDVMNE"
     gene            5760..6125
                     /locus_tag="VFQ23_15975"
     CDS             5760..6125
                     /locus_tag="VFQ23_15975"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9908150.1"
                     /translation="MNYQSINLQDKLTKFSEHWSPKIIAQMNDYHFKVVKVQGEFVWH
                     DHPETDEVFLVLKGQLEIHFREGKVSLNEGELYVVPKGTEHKPVAESECHILLVEPAG
                     TLNTSNVVDEKTAPNYAWI"
BASE COUNT         1688 a         1465 c         1451 g         1597 t
ORIGIN      
        1 tggaacgact ccaccacccg cgtcacccgt aaaattctcg aatctaaaca gtcgggaaaa
       61 atccaccgct actgggttga acacgaataa ggcgatgatc aggataatcg tcagtccaaa
      121 gatcaaatag ccgcttaaat cagtcacttt ggtggtgatg tcaataccat aatggttcag
      181 tagggcctgc gatattaata cgacagccac aaagaaaaac tgatgccagt aaccaaagct
      241 ggaagtatcc atgcccagtg tatcggtaag caatatacct ttgaagaata ctgtataaag
      301 caatacgtcc accgaggaca ctacgcagat caatcccacc aggttaaacc atgcagtcgc
      361 ccaaccccag gctttgccgc caaggtggga actccagtga tacaagccac ccgcagttgg
      421 aaaggaggaa gcgatctgtc ccagaccgaa ggcaacactt aaagcgagta tgccacccac
      481 caaccagatg acaaaggcac ccccggaacc aagcacattg aacgccgccg ggaaggcaga
      541 gataccccct gccagaatgc aaatgatcga gaatgaaatt gcgaaattcg agaagccgct
      601 catgcggcgg gcaagctctt gcgcgtaccc cagtttatgc agaacttgct tatcgtgtgt
      661 ctgacttttc tcagccatat ttctcatctc cttttggaat attgaaatga atcgatgcgg
      721 aaatcaattt tgctggcatt gtgtcataag cgctctcctc cgaatcagaa atacggcaat
      781 ccacaactca tctttccatt tttaataaaa cccgcacctt atgaaaagtg acgcaggagt
      841 tttttgcaaa catgttcact tgtctatacc catcttctta ctctcgactg gtaggttgaa
      901 tactcctcac cgaatctttt aaaaagatac ttctcctccg gtatgatcac aaacttatca
      961 aacatgaaaa tcatgaatgg agtcagcaat aagccccaat aagaaccggc gttgagcgga
     1021 attcctatca gcatgaatag gaatcctaga taaatcggat ttcgtgtaaa acgataaatt
     1081 ccaaatgtaa ccaatcgagc tgtagagccc tgatcattca tggttgtgcg ggcacgacgg
     1141 aaagccattg cagctcctat gccgaggaga aaccctacaa ttacaagcag gaatccaagg
     1201 gtctggagga agggtggagc cacccatggt aacggtacta aacgcgccaa cacaacagcg
     1261 atcacgatat ggactaatcc caaaagtgga ggaggaagtt tgaaattggc acgggcctta
     1321 ttaggttttg tcgtcatcct taatcctcag cgcatattga agcggaatat aattttgtca
     1381 gttgcaacac gggaatttgg aaaaggctat ggccgttaaa gtattgagtc gagaagcaag
     1441 aatacgttta gatcgaactc acgacgagaa ttctcttggc ttccgtaaaa tctggaacca
     1501 ttgtcccagc ctacgctgat atggaaaata tgcctcgcaa tccgcaataa cgaacgatgc
     1561 ttaagtcgcc tggggatttc tgtacctgta attccgcctt agacatcaac cccaacgcct
     1621 tgaaacttgc gtactgaatt tatcataaat attcgtgtga acactttcaa ttcttctgcc
     1681 aggatacttc tttcacttat ttggcctata tccaataaat gcgaccgaaa ggttccagcc
     1741 agtaatccac ttgaaagagg aggagtatat aatttgcctt caaattcaac gacaagattc
     1801 ccgatggtaa actcggtcag ttcgccgcga tcgttgtata agagaacatc gtcacaatca
     1861 gtacagccct gacgcgccat gtcgtaaact ttacgatgag ttgtcttatg aaacaaaaaa
     1921 atgttatcag tttcgattgg ctctctggcc agcttcacct tcagcggtga cgtgtgttgt
     1981 accggatgaa atgatttggc ttcggaattc aattcgccag ttttgtgcag taacaaccgg
     2041 acgcgctgag gtgaatcaaa ggtggaggat aacttatcaa gatatcgatc gatatctttt
     2101 tctagaatgg gaaagttaaa ataattcgca gagtccagca ttcgctcaac atgcttctta
     2161 cgcagaaaaa aaccgtggtc gggcgtccat aaaagcgttt caagcaacga gaattgggga
     2221 gtttgttcag ttagcacccg ggccttgagc aaggcttcct catattcatc cccgctggtg
     2281 gaatcccaga cgatgcctcc gccaacgcca tattcggctt gtttcgactc gcggtcgatc
     2341 aacccggttc gaatggcaac attaaatgat gctttgcgat tcggagcaac atagccaatg
     2401 ctgcctgtat agatttttct gggtgtcgat tcaagagcag tgatgatctt catcgtacgt
     2461 acttttggtg caccggtgat cgatgcgcag ggaaaaagcg ccgtgaaaat ttcagtcagg
     2521 gattcactgg tttttgctgt cacggtagaa gtcatctgcc agagtgtaga ataccgctct
     2581 gtatcaaaaa gatgtggaac ctctacgctt ccaagttttg cgatgcgtcc gacatcattg
     2641 cgtatcatgt caacgatcat cacgttttca gcccggtttt tctcagagct ttttagccat
     2701 tgggattgca gctcatcctc cagggttgtc cgtccgcgct tgactgtccc tttcatcggt
     2761 tggcaaatta ttctgtcgcc atcgagttga aaaaataatt ctggcgacgc cgaacagatc
     2821 acatagcgcc caatatcaat gtaagcggcg tgcttatttt ggttctgtgt cagatgcagg
     2881 aagaaatccc actcgctacc ggtgaaatcc gtctgaagcc gcatcgtata attaacctga
     2941 taagtccgcc catcagcgat atgactcctg atttgttcaa ttgctttgtt gtatgtttcg
     3001 cgatcaatcg tgggtttcca attcagccgg gatgtgctgt tatcgtgcgg atcgccaatc
     3061 tcgctaatcc actcaggtgc cgagtaaagt ccaaaccaaa gaagcgggaa atctccgtct
     3121 ataggccgca gcggcagatt ctcgtctgga ggcgcatccg acgcacgagt tcgaagggcg
     3181 aggtcaaatg cagaagccgc ctcataactt acaaatcccg cggcgtgcca gtcattgtcc
     3241 tgtgtcaacc gctcaatttc aagtagagcg ggtatcactt cgtcctgctt ttgcgcaagg
     3301 atcacgcgat gcggattggc aaaatgcagc catgtgctct cggctttaag aaggacttca
     3361 ttcgtcctga caaaggattg gggtttatta gctctggtca ttgttaaggc gccgttgatt
     3421 gtaatagctt ctcaatccaa agggtgattc tgttttttgc ggctcgcccc tataattcta
     3481 aaaacttgac ccaggagagt atgcatgaaa aattttagaa tcttattcgc agccgctatt
     3541 ctagtacttg tttcacttgc ctgtcagcga ttatctggag gctctggagg aggtggtaac
     3601 gagccacttt cgccgacaag tgtcggtgac tcgagcaccc cggcggtcgc tccaacggag
     3661 ccgagtggta acggcaatac gtccagtacg ggcttcccca tgacagccga tgcctacgat
     3721 gtcaccgaaa ttggtgatgg cagtttgctt tactatacta atttgtcact ggaagaagtc
     3781 atgactttct accgagccga atatacatcc caaggataca cagaacgcga aatcctcacc
     3841 gtggtttcag atggtgtttt cagcatggtc tttgatggtg atccaagcgg caagggagtc
     3901 gtcatccaaa gcgttgacct gggcgatggc agccgaactg tggcaattcg tcttgaagac
     3961 gtgtaaagaa tccattttca tacaaagcca tcgtcacaac caggcggtgg cttttcattt
     4021 cttcatgagg aagtttagtt ttgaactacg aaagtggctt ccgtccctgc tcatgatggg
     4081 cttgatcttt tggttttccg cgcagccttc gtataacctg cctgatttca actgggcaga
     4141 cagaattgtc aaaaaaggtg ggcatatact cggctatggt ttgcttgcgt gggggaactg
     4201 gtcggccctg gaagggaaac ctaacaggca ctggctggcg tggcttctag ccatccttta
     4261 cgcagtgacc gatgaatttc accaatcgtt tgtcgtgggt cggtttccat cggtttggga
     4321 tgtagtggta tttgataatc ttggtgcagt gatatcaatc tggctgctca ggcaaataaa
     4381 taaaaacgct atgccaaaat cagctcagat tatttagatt caggaagcct ttgagcgatg
     4441 gaaggcacta ctcaaactcc aattccattt cgtcgtcatc ctcccaggta tccattcgat
     4501 cgattgcaat atcatcgaag agtccctcct gatgagccgc gactcgatat cgtttgatga
     4561 gctccaataa cgccagaaag gtaaccacca cttcgagacg tgaagcattc gcgttaagca
     4621 gggcgctgaa cgttgaacgt tccacttcct taagcgtttt ggcgatcata tcaatttttt
     4681 cgcgaatggt aattcgcggc ggcgcaataa tcactccaag cggttgtttt tccttctcct
     4741 tggcgaaagc aacttcagca gccgtcacca gtctctcaag tgttagattc gaaagatcca
     4801 gcttgggctc tacctttgga ggaggggcga ttcgtaagta agttcgaagg tttgcgtttt
     4861 gttgattgtt taaccatccg cctatttcct tgaagcgttt atacaatttc aattgatcaa
     4921 caagggatct gcctgtatct tcctcgccag gggttggagc cggtggcctt ggcaacaggg
     4981 cttcggactt gatttgaagt aattttgcag caatgacaat gaaggcggaa atttcatccg
     5041 cattgatttg ttcaaggccc ttgatatatg acaaatactg atcggttacc aacgctaatg
     5101 aaacggacgt aatatccaat tccgcacgtt cgatgagatt taacaacaga tccaacggcc
     5161 cttcatatac aggggtttga acgttgtaat gtagctgtct gcccaacaag ttatccatac
     5221 ggctgaaatt atacccgtag acctaagtca ttaccgacta cgatataatc tcaacatgca
     5281 aaacaagttg actcatcttg atgaccaggg ccgcgcaaaa atggtcgatg tgggacacaa
     5341 gccagatacg gagcgtatcg ccgtggctcg atgcgaagtt catatgttga aagaaacgct
     5401 ggagctgatc cgtgcgggac agatcaaaaa aggagatgtg ctgacgatcg cgcagattgc
     5461 gggaatcacc gcttccaaac ggacctccga cctgatcccg ttatgtcatc ctctgcctct
     5521 caccaaagtg gatgtggacc tcgagctcga cgactcaatt cctggtgtcg tcatcaccgc
     5581 gactgcgaag accattggca aaaccggcgt cgaaatggaa gctctgaccg ccgtttctat
     5641 ctctgccctg acggtgtatg acatggcgaa agctgctgaa aaaaccatgc ggattcaaaa
     5701 tatccgattg gtcgaaaaac atggcggtca aagtggcgat gtgatgaatg agtaatagga
     5761 tgaactatca atcaataaat cttcaagaca aattgaccaa attttctgag cactggtcac
     5821 caaagatcat tgctcagatg aacgattacc acttcaaagt cgtcaaagtt cagggcgaat
     5881 ttgtgtggca cgaccacccc gaaaccgacg aagtatttct ggtgctgaag ggccagttag
     5941 agatccactt tcgagaagga aaagttagct tgaatgaagg cgaactgtat gttgttccga
     6001 aaggtaccga acataagcct gttgccgaaa gcgaatgtca catcctgctg gtcgaacccg
     6061 cgggcacatt gaatactagt aatgtcgtcg atgaaaagac cgcgccaaat tatgcctgga
     6121 tttagagaaa cgatgaatcg tgtaaaacta ttgctttttg ctactctacg tgaccgcgca
     6181 gggacaaagt ctatcgaact g
//
LOCUS       DASTJP010000546         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1289001, whole genome shotgun sequence.
ACCESSION   DASTJP010000546 DASTJP010000000
VERSION     DASTJP010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289001"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..922)
                     /locus_tag="VFQ23_15980"
     CDS             complement(128..922)
                     /locus_tag="VFQ23_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908151.1"
                     /translation="MPRRIFILFCLVLLSCTSPASQTIEPQQQLKTSFYLYRFDPPAF
                     IEYSEDFQLVEETSFAIPPNCGLFNTFPAPVGKFMLIELNCPNGQTVLFMDVESTSVT
                     QPVTDTDSHFLAWALDGTAAYLKIDSLGQPRVIRAFPEGKQEIIPINEYTYDLAPSGN
                     QNEFTFTFSRGLGQGSELWLAKRDGDSVEKLYADPLKYISFARWSPDGSQIAFIKIPD
                     SQVPFTVGELWVIPSTFDQGADGSTARKLADADAGHGYAANWSPDG"
     gene            1099..1872
                     /gene="surE"
                     /locus_tag="VFQ23_15985"
     CDS             1099..1872
                     /gene="surE"
                     /locus_tag="VFQ23_15985"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET9908152.1"
                     /translation="MHILVTNDDGIQAPGLLALAQAMQPLGKVTVVAPDRNWSASGHV
                     KTLERPIRVTETKLADGTPAFMSDGAPSDCVALPLLGFVEKIDLVVSGINPFSNIGHD
                     VTYSGTVTAAMEAVIAGVPGIAFSLASQDRPANTVAEYGPAGQIAALVTKKVIEDGMP
                     EGVVLNVNIPYLSVENIAGLAITRQGMRVYRDALDKRLDPRGRPYYWIGGEEPTGVNE
                     EGTDVGALARGYVSLTPLQLDLTNYKAMDVLRNWKWGNK"
     gene            1869..2333
                     /locus_tag="VFQ23_15990"
     CDS             1869..2333
                     /locus_tag="VFQ23_15990"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET9908153.1"
                     /translation="MTEIIEAYWQKFLSTLSSDSPYHTKSYSKGGYGDSPELMDELIQ
                     LVLIGKKTATCGSLWEWEAQGEPIPKIGDVWVELDGSGNPVCITETTEVTIRNYNEVD
                     AQFAHDEGEGDQSLNYWREAHKNYFSRVLLKIGKEFSEDMPLVCERFRVIYK"
     gene            2418..3407
                     /gene="mgrA"
                     /locus_tag="VFQ23_15995"
     CDS             2418..3407
                     /gene="mgrA"
                     /locus_tag="VFQ23_15995"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HET9908154.1"
                     /translation="MPYLASDSRYESMTYNRTGNSGLKLPPISLGWWYNFGGVDVFEN
                     GRAIARRAFDLGITHFDLANNYGPPPGSAEENFGTLFKQDFHPYRDELIISTKAGYDM
                     WPGPYGEWGSRKNLLASLDASLKRMGLEYVDIFYSHRPDPETPLEETMGALDSAVRQG
                     KALYVGISSYNPEQTRRAAHILRELGTPCLIHQPSYNMFNRWVEDGLLDVLVEEGIGC
                     IAFSPLAQGQLTDRYLKGIPSGARATKTERVWLTPDNVKENLPKVEKLNQIAQARGQS
                     LAQMAIAWVLRKPAVTSALIGASSAKQLEDNIAALNNSKFTEDELDRIEEILK"
BASE COUNT          920 a          836 c          889 g          819 t
ORIGIN      
        1 ttaaatgagt gacctgagtc aattcaccac tctccacgtc aacgagatat atattactga
       61 tcaacgattc aactgattga ttagcatttt cctgatctgg attctcgcgt acgacaaacg
      121 cgatttgtca cccatccggt gaccagttcg cggcgtaccc atggcctgca tccgcatctg
      181 ctaatttacg agcggttgaa ccatctgcac cctgatcgaa agtcgaaggg atgacccaca
      241 actcccctac tgtaaatgga acttgcgaat cagggatttt gataaaagca atctgacttc
      301 catccggcga ccagcgagca aacgatatgt acttgagtgg atcagcatac aacttctcga
      361 cactatcacc atcgcgtttc gccaaccaaa gctcacttcc ctgccccagc ccacgcgaga
      421 aggtaaaggt aaattcattt tgatttcctg agggcgcaag atcatatgta tattcgttga
      481 tcggaataat ctcttgctta ccctccggaa aagcgcggat aactcgtggt tgccccaatg
      541 aatctatttt gagataggcc gcggtgccat ccagtgccca cgccaggaaa tgactatccg
      601 tatctgtgac aggttgagta acacttgtcg attcgacatc catgaataac accgtttgcc
      661 cattcgggca gtttaattcg atcagcatga acttcccaac cggagccggg aacgtgttga
      721 ataatccaca attcgggggt atcgcaaacg aagtctcttc gacaagctga aagtcttccg
      781 aatattcaat aaaggcgggc ggatcgaagc gataaagata gaatgaagtt ttaagctgtt
      841 gctgcggttc tattgtttga gaggcagggg acgtgcagga aagaagtacg aggcaaaaca
      901 aaataaatat tcttcggggc atagaggtat tatacaggcg ggttataatc caaaagtaac
      961 atgttggtcg agtagcccga tgcttgtcgg gcatatcgag accaacatga aataagaata
     1021 cttttgtaaa ttggtggttt cgatacgtcc cgcgaaaagc acgcgggact actcaaccat
     1081 cgaagttggg atgtaacaat gcacatactt gtcaccaatg atgacggcat tcaggcacca
     1141 ggtcttctgg cgctggcgca ggccatgcaa ccacttggca aagtgactgt cgtcgcgcca
     1201 gatcgcaact ggtcagcctc cgggcatgtc aagacgctgg agcggcccat tcgcgtcacc
     1261 gagacgaagc tggcagatgg caccccggct tttatgtccg atggagcgcc gtctgattgt
     1321 gttgcccttc cattgctggg ctttgttgag aaaattgatc tggtcgtttc gggcatcaat
     1381 cctttctcga atattggcca tgatgttacc tactccggta cagtgacagc cgccatggaa
     1441 gcggtcatcg ccggcgtgcc gggcatcgct ttttcactgg catcacaaga cagacccgca
     1501 aatactgtgg cagagtacgg tcctgcgggc cagatcgctg cgcttgttac aaagaaagtg
     1561 attgaggacg gtatgccaga gggagtggta ttgaatgtaa acattcccta tctcagcgtt
     1621 gaaaacattg cagggttggc catcaccaga cagggaatgc gcgtctaccg cgacgcgctc
     1681 gataaacgtc tcgacccgcg cggccgtccc tattattgga tcggtggcga agagcccact
     1741 ggtgtcaatg aagaagggac agacgtcggc gcgctggcac gaggctatgt gtccctcacg
     1801 ccattgcagt tggatctgac aaattacaaa gccatggacg tgttgaggaa ttggaagtgg
     1861 ggtaataaat gacggaaatc atcgaagcgt attggcagaa atttctatcc acgctttcat
     1921 ctgattctcc atatcacacg aaatcctatt ccaaaggcgg gtatggggat agcccggaac
     1981 taatggatga actcattcag cttgtcttga tcgggaagaa aacagcgacg tgtggcagtc
     2041 tgtgggaatg ggaagcgcag ggcgagccaa ttccaaaaat tggagatgtt tgggttgagt
     2101 tggacggaag tggaaatccc gtgtgtatta ctgaaactac cgaagtgacg attcgaaact
     2161 acaacgaggt ggatgcgcaa tttgcacatg atgaaggcga gggcgaccaa tctctgaatt
     2221 attggcgcga agcacataag aattacttct cgcgggtgct tctgaaaatc ggtaaggagt
     2281 tttcggaaga tatgccattg gtatgtgaaa ggtttcgagt gatttataaa taatatcgga
     2341 agcaaacaat cgataaatta caaacattgg tcttacaagg attccaaaat gacgatgacc
     2401 ctttataagg aaacacaatg ccctatctag cttcagactc ccgttatgag tccatgactt
     2461 ataaccgtac cggcaacagc gggctcaaac ttccgcccat ttcgctgggc tggtggtaca
     2521 acttcggcgg tgtggatgta ttcgaaaacg gacgcgccat cgcacgccgt gcctttgatc
     2581 tcggtatcac acatttcgat cttgccaaca actacggccc gccgccgggt tccgcggagg
     2641 aaaactttgg caccctgttc aagcaggatt ttcatccgta tcgcgatgaa ttaattatct
     2701 ccacgaaagc cggctatgat atgtggcccg gtccctatgg ggaatggggt tcgcgcaaaa
     2761 acctgcttgc cagcctcgat gcatctctca aacgtatggg tcttgaatat gtagatatct
     2821 tttatagtca ccgccctgac cctgaaacgc cgctcgaaga aacgatggga gcactcgact
     2881 ctgccgtgag acaaggtaag gctctctacg tcggcatctc ctcctacaat cccgagcaga
     2941 cgcgccgggc cgcgcacatc ctgcgagagc tggggacgcc ttgtctcatc catcaaccct
     3001 cctacaacat gttcaatcgt tgggtggaag acggattgtt ggatgtgctg gtcgaggaag
     3061 ggattggatg tatcgccttc tcacctctcg cgcagggaca actgactgac cgttatttaa
     3121 aaggaatccc atctggagcg cgtgccacca aaacggaacg cgtctggctg actcctgaca
     3181 atgtaaaaga gaatctcccc aaagttgaaa agctcaacca aatcgctcag gcacgcgggc
     3241 aatccctggc acaaatggcg attgcctggg tcttgcgcaa accggcggtc acatcggcgt
     3301 tgattggcgc aagtagtgca aagcaactcg aagataacat cgcggcgttg aacaattcga
     3361 agttcactga ggatgaattg gacaggattg aagagatttt aaaatgatat cgttggtcga
     3421 gtacgacagt tgtatgtgag atatcgagac cacaagttaa ctga
//
LOCUS       DASTJP010000547         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1493589, whole genome shotgun sequence.
ACCESSION   DASTJP010000547 DASTJP010000000
VERSION     DASTJP010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493589"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFQ23_16000"
     CDS             <1..423
                     /locus_tag="VFQ23_16000"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9908155.1"
                     /translation="GVKMADIKTKVNDASVEEFLSKVENEQKRKDSFEIVKIMKQVTK
                     KEPKMWGPAIIGFGSYHYKYESGREGDMPQLAFSPRKQNITLYIGVGDDSNNPLLKKL
                     GKYTTGKVCLYIKKLDDVDRKVLQELIADSFERAPESG"
     gene            501..893
                     /locus_tag="VFQ23_16005"
     CDS             501..893
                     /locus_tag="VFQ23_16005"
                     /inference="COORDINATES: protein motif:HMM:NF042549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6632 domain-containing protein"
                     /protein_id="HET9908156.1"
                     /translation="MSEANRIKYLRIALTVIGLVSLFGFWPLTIIWPSGWSWHAEGRS
                     YYLEMIIGIYATLGICLILAAREPLKNLSLIWFTVWSSLVHGGIMAVQSFDGMNHRGH
                     LYGDVLVLFVVAALLAFLTPRKDSFVGP"
     gene            1194..2126
                     /locus_tag="VFQ23_16010"
     CDS             1194..2126
                     /locus_tag="VFQ23_16010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9908157.1"
                     /translation="MNTSQLIESYSFSKLILDSIREQIVVIDQGGKILFCNRAWVEFG
                     EKNGYLSKYWLGTNYLKACDDAATSGDELGKKAAHGIREVIAQRVDEFYLEYPCHSPD
                     EKRWFMMRVTPLVWEGEGYLVVSHRNITERKMIEEQIVNLSRLDGLTNLTNRKYFDEF
                     LDEEWRRCARLNLPITLALLDVDHFKLLNDYYGHQAGDDCLIKIGEAMSKIINRPSDL
                     SARFGGDEFSLTFGNTTIEQALVLINKLVKDIHDLKIPNEQSPTNPLVTVSIGLGMMK
                     PNRENNIKDLISLADKNLYLAKDLGRNRVIFKAV"
BASE COUNT          623 a          502 c          546 g          610 t
ORIGIN      
        1 ggagtcaaaa tggccgacat aaaaacgaag gtaaacgatg cgagcgttga ggaatttttg
       61 agtaaggttg aaaacgagca aaagcgcaag gatagctttg agatcgtaaa gatcatgaag
      121 caggtgacga agaaagagcc aaaaatgtgg ggaccagcca ttatcggctt tggctcctat
      181 cattacaagt atgaaagtgg gcgggaaggc gatatgcctc agcttgcatt ctctcctcgc
      241 aaacagaata tcacgctcta tattggggtg ggcgatgact ctaacaaccc tctcctcaag
      301 aaacttggta aatacacaac cggcaaggtt tgcctgtata ttaagaaact ggacgatgtg
      361 gataggaaag ttttgcagga actgatcgcc gactcatttg aaagagctcc tgaatctggg
      421 taggctttaa gtcgtctcgt caatttcatc ctgagcagat tgaatagcag ctggattcac
      481 atttcgccaa cggagaagat atgagcgaag caaaccgcat taagtattta cgcattgcgc
      541 tgacggttat cgggctggtt tctcttttcg gtttctggcc tttaaccata atttggcctt
      601 cgggatggtc gtggcacgcg gagggacgat cctattatct ggagatgatc atcggtatat
      661 acgctactct tgggatatgt ctcatccttg ctgctcggga gccactcaaa aacctaagtc
      721 tcatttggtt caccgtgtgg tcaagcctgg tacatggcgg gatcatggcg gtgcaatcat
      781 tcgacggcat gaatcacaga gggcatctct atggagacgt cctggtactg ttcgtagttg
      841 ccgctctatt agcattcctc actccacgca aagactcgtt tgtcggacct tgattttctg
      901 tcagactttg atggagataa atacgcgttt cacctgactg tctccattgg aacagttttc
      961 ccctttgttg cccgacacag caccccttgc tggctgctgg ggttaatcaa catgtagtta
     1021 cggttgatat cacccattgc ccttgcaccg aaaattgtca aaagcagcag cgggccgctc
     1081 cctcactcca gcaccgcgtt ttctgagcgt atttacagat tttgttggtg actactgtac
     1141 cggtctttca ctccttaacc gcaggtagtg cttcccgtta ggcttggaaa aaaatgaata
     1201 catctcaatt aatagaatct tatagttttt caaaactgat tcttgactca atcagggaac
     1261 aaatcgtcgt aattgatcag ggtggcaaga ttctgttttg caatagggcc tgggttgaat
     1321 tcggggagaa aaatggctat ttgtcaaaat attggctcgg aaccaattac ctcaaagcgt
     1381 gcgatgatgc agcaacttca ggcgatgaac tcggtaaaaa ggcagcgcac ggcattcgag
     1441 aagtaatagc acaaagagta gatgagtttt acctcgaata tccctgtcat agcccggatg
     1501 agaagagatg gtttatgatg agagttacgc cccttgtctg ggagggggaa ggttaccttg
     1561 tcgtttcgca tcgaaatatt acagaaagaa aaatgattga agaacagatt gtcaatttat
     1621 cgcgcctcga tggattgaca aaccttacca atcgaaaata cttcgacgag ttcctggatg
     1681 aggaatggcg gcgttgcgcc cgattaaatc ttcccataac gctggcactc ctggatgtag
     1741 accacttcaa attattgaat gactattacg ggcatcaggc cggagatgac tgtctcataa
     1801 aaattgggga agcgatgagc aaaatcatta atcgcccaag cgacctcagt gcccgatttg
     1861 gcggtgacga attttcacta acttttggca acactacgat tgagcaggct ctggtgctca
     1921 ttaataaatt agtgaaggac attcatgacc tgaagattcc aaatgaacag tcaccgacca
     1981 accctctggt aactgttagc atcgggctgg gaatgatgaa accaaatcgg gaaaacaata
     2041 tcaaagatct tattagtttg gcggataaaa atctttatct ggcaaaggat cttggtagaa
     2101 atcgcgtgat atttaaagcg gtttgaagtc tctttactta cccctatgcg tatcaattgc
     2161 tacaggtttc cacagcgcgt tgcgtttatt tccgcttcga tagcaactcc caacttactt
     2221 agctgtcact ccctcctctg gatagaacac gatcgggtcc gcttcgcggc cgcacaccgt
     2281 t
//
LOCUS       DASTJP010000548         4732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_891063, whole genome shotgun sequence.
ACCESSION   DASTJP010000548 DASTJP010000000
VERSION     DASTJP010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891063"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..2088
                     /locus_tag="VFQ23_16015"
     CDS             292..2088
                     /locus_tag="VFQ23_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908158.1"
                     /translation="MTTKIVPPAFITHSEELLDKGYDPKVARGILNFVKPYSGRMFFA
                     LILMIIVTAAAVSWPYFVKLAIDEGITKNDPIALRNIVLTFLVVSIIQFVTNFFRVRI
                     MSRVGQHVLYDVRTAMFDHLQNLSLSFYNRYSVGRVITRVINDVGTLREFITWAVLAI
                     VRNLLGIIGTIIAMVALNIRLSLLTLAVIPLMILATILFRRAARQNYRKVRAAVSWTN
                     SVLAENVNGVRVVQAFSRQSHNYLNFKDYVNRYFLETSLDAAKVASVFTPIVDVLGAI
                     ATALVVYIGGRAVLGEEITAGVLIAFVLYIDRLFDPIRDLSRRFDTLQSTMAGGERIL
                     ELLNSPVEVQDAATAIEMRPIKGDVRFENVSFHYSDDPTLVLDGIDLDVQAGETVALV
                     GETGAGKTTIVKLLTRFHDPTAGHVRVDGVDLRTVTQASLRKQMGMVLQDPFLFNGTV
                     KENILFGRLDASDEEVVAAAKAVGANDFIMSLKNGYETSVEEGGALLSVGQRQLISFA
                     RALLADPRILILDEATSSVDTQTEQIIQKALATLLDGRTSFVIAHRLSTITKADRIVV
                     IHDGKIIEQGTHTQLLANQGMYYELYKTGFQD"
     gene            2085..2369
                     /locus_tag="VFQ23_16020"
     CDS             2085..2369
                     /locus_tag="VFQ23_16020"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HET9908159.1"
                     /translation="MIRPTIFFRVHAIQRMFERKISEKAVLNALEIGETIEDYSSEMP
                     EKSRLILGFQGTRPLHAVISENQERNELTVITVYHPNPDQWSKDFKSRRA"
     gene            2819..4555
                     /locus_tag="VFQ23_16025"
     CDS             2819..4555
                     /locus_tag="VFQ23_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908160.1"
                     /translation="MNSRTRKFLSYYRPYKRLFFTDMACAFIVSVTALLFPLCANYIT
                     QNLLESGAADAEIRIYGMGAVMLLLLLVHVFCNMFVDYQGHMMGAMMERDMRSELFEH
                     YQKLSFGFYNEQKTGQLMTRLTNDSFDLAELFHHGPEDIVISLLNFLGAFLIMLTIDV
                     GLALIVVLFLPIMAIYTIYFNKKMQVALRRSHDRIGDINAQVEDALSGIRVVQSFTNE
                     EIENAKFVHENNRFLASRSEGYKSETYFTAGMISFVQLLTIAVIVFGGISILNKSLGL
                     ADLITFLLYVGLLIEPIRRFSNFTRLYQEGIAGFDRFMEVLEVKPDIQDAPNAIELTH
                     VQGNIEFQDVTFKYKEDHDYVLRNISLNIKAGEYVALVGASGVGKTTLCSLIPRFYEI
                     NAGKILLDGRDIRDVQLRSLRKNIGLVQQDVYLFAGTVADNIRYGKLGASRDEIIEAA
                     KQANAHSFIMELPKGYDTDIGQRGVKLSGGQKQRLSIARVFLKDPGIIILDEATSALD
                     NESERAVKDSLEKLAQNRTTLVIAHRLSTIRNAQRIVVLTDNGIEEQGTHEELLARDR
                     SYANLYNMQFII"
BASE COUNT         1237 a         1034 c         1207 g         1254 t
ORIGIN      
        1 actgaattat aaagaagcat ttaaatggga gcatcaaatt aaaggctgga gccgcgccaa
       61 gaaagaggca cttattcgtg gtgatattga aggtatccat gaaattgtga aaagtgaaag
      121 aaagcgcaga tcaatattaa ggaaaaacac tcgcgctgag cggagtgacg agtgaaacga
      181 ggaacgaaga cgaagtgcga atagccaacg taatcttatt ttttgtgaaa cctgcgcttc
      241 gtctaccgag cgaaaaacca tcgctcctcc gctcagcgcg aaataaataa tatgacaacc
      301 aaaatcgtcc caccagcatt tatcactcac tccgaggagc ttcttgataa gggttacgac
      361 cccaaagttg cgcgcggcat actaaatttt gttaaaccct attcagggcg aatgttcttt
      421 gcgttgattc ttatgatcat cgtgacggct gctgccgtat cctggccgta cttcgtcaaa
      481 ctggcgatcg atgaaggaat tacaaagaat gacccaattg cattgcgaaa tattgtcctg
      541 acgtttcttg ttgtatcgat tatccaattt gtgaccaact tcttccgcgt gcgaatcatg
      601 tctcgcgttg gtcaacatgt actgtatgac gtgcgcaccg cgatgttcga ccatttgcaa
      661 aacctgtcac taagttttta caatcgctac agcgtggggc gcgtcattac ccgcgtgatc
      721 aacgatgtgg gtaccttgcg tgagtttatc acctgggcgg tgctggcgat tgtccgtaac
      781 ctgttgggca ttattggaac catcattgcc atggtggcgc tcaacattcg tctctcgctg
      841 ctgacacttg cggtgattcc gctgatgatc ctggcgacca ttctgtttcg ccgtgcggcg
      901 cgacagaatt atcgcaaagt acgcgcggcg gtctcatgga ccaattctgt gctggcggag
      961 aacgtgaatg gcgtgcgtgt cgttcaggca ttttctcgtc agtcccataa ctatctgaac
     1021 ttcaaagatt atgtaaatcg ctattttctg gagactagcc tcgatgcggc aaaagtggct
     1081 tccgtgttta caccgatcgt tgacgtgctt ggtgcaatcg cgacggcttt agtcgtttat
     1141 atcggtggac gagctgtgtt gggcgaagag atcactgcag gagttttgat cgcctttgtt
     1201 ctttacattg atcgtttatt tgacccgatc cgcgacctga gtcgtcgctt cgatacgctg
     1261 cagtcgacga tggcaggtgg agaacggatc ctcgaactcc taaattcgcc agtcgaggtt
     1321 caggatgcgg caactgccat tgaaatgagg ccgatcaaag gtgatgttcg attcgaaaat
     1381 gtctcctttc attactcgga tgatccgact ctggttctcg atggaatcga tttagacgtc
     1441 caggcgggtg agacggtcgc tctcgtgggc gaaaccggtg caggtaagac gacaattgtc
     1501 aaactcctga cgcgctttca tgatccgacg gctggtcatg tccgcgtgga cggggtcgac
     1561 cttcggacgg tgacccaggc gtcccttcga aaacaaatgg gcatggtttt acaagaccca
     1621 tttcttttta acggcacggt aaaggaaaat attctgtttg ggcggctgga tgcaagcgac
     1681 gaggaagtgg ttgcagcggc aaaagctgtg ggtgcgaatg actttatcat gagcttaaaa
     1741 aatggatatg aaacctctgt ggaagaaggc ggagcactcc tgagcgtggg tcagcgacaa
     1801 ttgatctctt tcgcccgtgc gcttctggct gatccgcgga tattgatttt ggatgaagcg
     1861 acttcatccg tggatacaca gaccgagcag attattcaaa aggcgctcgc cactctgctg
     1921 gatggacgga cctcgtttgt gatcgctcat cgcctgtcaa cgatcacgaa ggcggatcgg
     1981 attgtcgtca ttcatgatgg caaaatcatc gagcagggaa cgcacacaca gctactggca
     2041 aatcagggta tgtattatga gttgtacaag acaggatttc aggattgata cgccccacaa
     2101 ttttctttcg cgttcatgcc atccagcgca tgttcgagcg taaaatttct gagaaggctg
     2161 tgctaaatgc ccttgagatc ggggagacca tcgaagatta ttcatcggaa atgcctgaaa
     2221 agagtcgttt gatattgggc ttccagggga cacgtccgtt gcatgccgta atttcagaga
     2281 atcaagagag aaacgagcta accgttatta ccgtctatca tcctaatccg gaccaatgga
     2341 gcaaagattt taagagtcgc agagcatgat ctgcctggtt tgccaacaag cggaaactga
     2401 cgacggatta acatctgtca attttgtgcg tggcgagatg aggttgctgg tacgcagcgt
     2461 gcctgcgcga gtttgtccaa gttgtggaga ggcttacctg cacgaagagg tcgctgtgcg
     2521 attattgcag gaagcaaaga aaatatctga ggcgggaatc agagagcatg gaattgagta
     2581 tacccaaggc ccgtagacca attcttgacg cacccaacct caaatgataa actcgccgta
     2641 tcttattttt gaaaggaggc acctacaaat gaaagcgaag ttgacatatt catttgttct
     2701 ccgcgcgcct cctgaaggtg aagtaacggc ttgagtcgtt aaaattgttc ttaataacac
     2761 accgcaggct tgtatcgccc tgcggttttt tgtttaattg caactctgag gaaattttat
     2821 gaattctcga accaggaaat ttttgtccta ttacagaccc tataaaagac ttttttttac
     2881 ggatatggca tgcgcgttta tcgtatcagt gacagccttg ctttttccac tatgtgcaaa
     2941 ttacattaca cagaatctcc tggaaagtgg tgctgccgac gcagaaattc ggatctatgg
     3001 aatgggggcg gtgatgctgc tgttactcct tgtgcatgtt ttctgcaaca tgttcgtcga
     3061 ttatcagggg cacatgatgg gtgccatgat ggaacgcgat atgcgcagtg aactttttga
     3121 gcattaccaa aaattgtcgt ttggttttta taatgagcag aaaacaggtc agttgatgac
     3181 gcgtctaacc aatgactcct tcgacctggc tgaacttttt catcacggac cggaagatat
     3241 cgtcatttcc ctgttgaact tccttggcgc atttcttatt atgctcacca tcgacgtggg
     3301 actcgctctg atcgtcgttc tctttctgcc gatcatggcg atttatacga tttatttcaa
     3361 taagaaaatg caggttgccc tgcggagaag ccatgaccgt atcggtgata tcaatgctca
     3421 ggtagaagat gctctatcag gcatccgcgt ggtgcagtca tttacaaatg aagagatcga
     3481 gaacgcgaag ttcgttcatg aaaataatcg ttttctggct agcagaagcg agggctataa
     3541 gagtgaaact tactttacgg cggggatgat ctcctttgtt caattgctga ccattgccgt
     3601 catcgtcttc ggtggaatta gtatcctgaa taaatcgttg ggactggctg acttgatcac
     3661 attcttactg tacgtcggcc ttctgattga acccatacgc agatttagta acttcaccag
     3721 gctgtatcag gaaggaattg ctggctttga tcgattcatg gaagttctcg aggtgaagcc
     3781 agatatccag gatgcaccca atgcaattga actgacacac gttcagggaa atatagaatt
     3841 tcaagacgtg accttcaaat acaaggaaga tcatgattat gtgctgagaa atatttcact
     3901 gaacataaaa gcaggtgagt acgtggcgct ggtcggtgcc tcaggggttg ggaaaactac
     3961 gctttgctca ttgatccctc gtttctatga gatcaacgcc ggaaagatac tactcgatgg
     4021 gagagatatt cgggatgttc aacttcgttc gctaagaaaa aacataggtc tggtgcagca
     4081 agacgtgtat ttgtttgctg gaaccgtcgc agataacatc cgctacggca aattgggcgc
     4141 aagtcgggat gagatcatcg aagccgccaa acaggcgaat gcacatagct tcatcatgga
     4201 gttgccaaaa ggatatgaca ctgatatcgg tcagcgcggc gtcaaactat cgggtggaca
     4261 aaagcaacgt ttgagcatcg cgcgcgtgtt cctgaaagac ccaggcatca tcatattgga
     4321 tgaagcgacg agcgctctcg ataacgagag cgagagagcc gtgaaggatt cgttagaaaa
     4381 gctggctcag aaccgtacaa cgcttgtgat tgcccatcgc ttatccacga tcaggaatgc
     4441 acagaggatt gttgtactca ccgataatgg aattgaagag cagggcaccc atgaagagtt
     4501 acttgccagg gatcgaagct atgcaaattt atacaacatg caattcataa tatagtcaat
     4561 accaaattgg cataagtgac tccctctccc cattgggaaa gaggtagggg tgatggctaa
     4621 atggaacgca atccctttac gcggtgtatt tgtctaaagg agaccatatg cgccaaaaac
     4681 ttcaatgggt tgtgctaatg ggattatttt tatcctcttg tagtggagtt gg
//
LOCUS       DASTJP010000549         7552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1023095, whole genome shotgun sequence.
ACCESSION   DASTJP010000549 DASTJP010000000
VERSION     DASTJP010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023095"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..818
                     /locus_tag="VFQ23_16030"
     CDS             444..818
                     /locus_tag="VFQ23_16030"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9908161.1"
                     /translation="MNVKGIVWLGSRTANFDAMLHMYRDVMGLPVTHEEPGFVVMELP
                     NGDLVEIFGDDSPDNMFFTHPVAGFLVDDIAAARAEMESQGIEFIGSIEGEAGGYSWT
                     HFRAPDGFVYELTYSPNHPALR"
     gene            complement(842..1507)
                     /locus_tag="VFQ23_16035"
     CDS             complement(842..1507)
                     /locus_tag="VFQ23_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908162.1"
                     /translation="MSIKVLIADDHVFYREGVRALLSNSPDITVVDEASNGDEAITKA
                     NELKPSVILMDIKMPGTNGIEATRRITQAEPTIGILVITMFDDDDSVFAAMRAGARGY
                     LLKDADKDELVRAIVAVERGEAIFSPAIAQRMIQYFSAAPSMSSGRKPSGEFSELTER
                     ELEILNLIARGDNNNTIANKLSLSIKTVQNYVSSILTKLQVADRAQAIVRAREAGFGK
                     SEK"
     gene            complement(1508..3607)
                     /locus_tag="VFQ23_16040"
     CDS             complement(1508..3607)
                     /locus_tag="VFQ23_16040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9908163.1"
                     /translation="MMISPTLAVASPAPRWLMVARAFQILSICLAIGMFIISIPLNYE
                     QRVTVCEVEPCLPNQLSLRSVQALEDVGMSVNDLVTQTIALDIIVAATYTVCAIVIFV
                     RKPDEPLTIFVTIMLVIFGTATFTGGIRGIGLVYPALNGLINFFELLGSFSILAFLFV
                     FPNGRFTPRWGVALLLIWSLLQILPRYFADSPLNLAKTNPNLYGILFTGGILSGIAAQ
                     IYRYLRVSNIVERQQTKWVVYGLTIAIVGYLITRELGRLNTDPLGTGLPYSMALGITA
                     NLIILILPISISLAVIKYRLWDINPIINRTLVYGALSASTIALYILAVGFASRYVQGA
                     NLIVSFIVTGVIAILFEPLRERLQRAVNRLMYGERDDPATVLTRLSRRLDSALAPDAV
                     LQTIVETLAQTLRLPYAAISLSDDTPAPRFASSPNKPPTQLISLPLTYQSERVGELSL
                     APRAEGELFSTADMNLLTLIAGQAGIAAYNLRLTQDLQRSREKLVTAQEEERRRLRRD
                     LHDGVGPTLASLSQRIDTAAEMVNIDPQASVELLKELKGQVRGSVSEIRRLVYALRPP
                     ALDEFGLVTAIREHVAPYTGPNGLQVTFDITDPMPPLPAAVEVAAYRIVLESFTNLIN
                     HAQASACDINMKFEDGSLLLEISDNGQGLPSNNHAGVGFTSMRERAAELGGHWNVENI
                     STGGTRVQAWLPIAKEE"
     gene            3710..4078
                     /locus_tag="VFQ23_16045"
     CDS             3710..4078
                     /locus_tag="VFQ23_16045"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9908164.1"
                     /translation="MDPTQEVLNVEHAWTEAHLHGDVQTLEEIMAFDYVKIQPDGSLS
                     DKQATLASYQPDQREWEVARGDEYDVRVYGDTAVVIGRWTARGINHGESFDYAARFLS
                     IYVRRDGRWQMVAEQSTDIK"
     gene            complement(4097..5716)
                     /locus_tag="VFQ23_16050"
     CDS             complement(4097..5716)
                     /locus_tag="VFQ23_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908165.1"
                     /translation="MKTLNLIYHMLRADFLERTRRSSFFVTLGLAVLIGYIYIPPADS
                     STLMLALGPWRGAYNSAWIGSVFGILIVISMPVLGFFLTKNTIERDRRSRVGQILATT
                     PMSKPIYTLGKWLSNMAVLTAMLVVLNLMAIVMQLLRGEIFPIDYWALSAPIWLMGFP
                     IIALTAAMAVLFESIPFLSGSAGNVIYFLGWYIFMDNLAVPGIFRYNIGVVEPWLDPL
                     GVTGLLAALQSVGQQVAPQFSGHINFGGAEFGVVPQVVTFAGIQWSLTIMLGRLAWFG
                     IAGLLALAASFPFDRFDPARTSVDGTSVLKSLLPNRPRRQPLAEPAFAESKPVNLTPI
                     NHRNSRSRFLSLLNAEFKLLVKGKPWWWYLVAAFISLLGLIGPPGGSDVTLALAMIWP
                     VIAWSALGMRETFYETYKLTYSSAHPLGHQFMASWFSGVLLATLAISGATVRMVIEGG
                     ADRLPLILLAVLFVPSLAMALGTWSNNSRLFEVVYLLWLFNGVNGAVPLDFMQVLQTS
                     PNPILLAAYFALTVVLFAASLAGRWKQLYAN"
     gene            complement(5713..6600)
                     /locus_tag="VFQ23_16055"
     CDS             complement(5713..6600)
                     /locus_tag="VFQ23_16055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908166.1"
                     /translation="MELLINHVGKKYSGEVWGLKEFSLHLKPGILGLLGPNGAGKSTL
                     MNILATITRPTQGAVSWNGVDIVRRPDELRQILGYLPQSFGIYPNLSAQEFLEYMSAV
                     KGLDGRTARRRIDELLQLVNLTEVRKRPLGGYSGGMKQRIGIAQALLNDPQLLIVDEP
                     TVGLDPEERVRFRNLIADLAGERIIILSTHIVSDVEATATRIVIANHGRLIMDTNSEE
                     LLRVVEGKVWEWIVPTSEFAEVKSRHLVSGTLRRTDGMQMRVISRAAPAQDARPVTPT
                     FEDAYLKLISNGNHGKKGL"
     gene            6827..>7552
                     /locus_tag="VFQ23_16060"
     CDS             6827..>7552
                     /locus_tag="VFQ23_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908167.1"
                     /translation="MASETYVIETQGLTKSYKGVNALQPLNLRVRQHSICGFLGPNGA
                     GKSTTIKLLLGLARPTGGSAKVFGMDIVRDSTDIRKRVGYLAQDPRYYDHMTARETLR
                     FTARFFYSGPEKRMEDRIDETLELVGLSDKADRSIKGFSGGERQRLGIAQAQINYPDL
                     LILDEPAASLDPMGRRDVLEVMERLRKHTTIFYSTHILEDVQRVSDTVAILNHGRLIA
                     QAPIAELTSGKDGIIYSLKVKGDG"
BASE COUNT         1869 a         1880 c         2066 g         1737 t
ORIGIN      
        1 ggttaagaga agtaaattcg gtggtcatct tttccttttt atttatgtca ttgcgagcga
       61 gcgtcgagag cgaagcaatc tcctactatg aatcaggtgc ttgcttcgga caacaacgct
      121 ctcgcaacga catctatatg gattgtttcg atgactccgt accacttctt cttgccaatc
      181 aaagcttggc ttttcagcgc agcttgccca acaaaaaagc ccgatcaaat ttcgtcgggc
      241 attcgttcag agtgatcaaa acaatttccc aaaaatcttg ctccatcagg agcagagaga
      301 gtatatcatg gtttgagttt cgtagggcta ttcgtatccg aaacctggaa ttggtgttgg
      361 aaatttggga aggtttgata gtcttacgat cagggaggaa gcttcaggat tctgtggaac
      421 tatcgttaac aaggagaaaa ccgatgaacg tcaaagggat tgtttggctt ggaagccgca
      481 ctgcaaattt cgatgcgatg ctgcacatgt atcgggatgt gatgggctta ccggtcaccc
      541 atgaagaacc gggttttgtg gtgatggaat tgccgaatgg tgaccttgtt gaaatctttg
      601 gtgacgattc acctgacaat atgtttttca cacaccccgt tgctggcttt ttggtcgatg
      661 atattgccgc ggcgcgcgca gaaatggaat cgcagggaat cgaatttatc ggttcgatcg
      721 aaggggaagc gggtggctac tcctggacgc acttccgcgc accggatggg tttgtttatg
      781 aactcaccta tagccccaat caccccgcat tgcgttagcc gattgccctt ctttgctttg
      841 attacttttc gcttttccca aacccagcct cgcgcgcgcg gacgattgcc tgcgcgcggt
      901 ctgccacctg taattttgtg agaatactcg aaacataatt ctgtacagtc ttgatgctca
      961 acgatagttt gttcgcaatc gtattgttgt tgtctccacg cgcgatcaaa ttcaaaatct
     1021 caagctcccg ttcagttaat tcagaaaatt cacctgacgg tttccggccg gatgacatag
     1081 atggcgcggc agaaaaatat tggatcatgc gctgggcaat ggcgggacta aaaatcgctt
     1141 cgcccctttc cacagcgacg atggctctca ccagttcatc cttatctgcg tccttgagca
     1201 gataaccgcg cgcgccggcg cgcatcgccg cgaaaacgga atcatcatcg tcgaacatgg
     1261 tgatgaccaa tatgcctatg gtaggctccg cttgtgtgat cctgcgggtg gcttcgatgc
     1321 cattcgtgcc gggcattttg atatccatca gaatgacact gggcttgagt tcgtttgcct
     1381 tggtaatggc ttcatccccg ttgctggcct cgtcaacgac ggtgatatct ggtgaattgc
     1441 ttaataatgc acgcacgcct tcgcgataga aaacatgatc atcggcgatc aagaccttga
     1501 ttgacattta ttcttccttt gcgataggca accacgcctg gacgcgcgtc ccgcctgtag
     1561 atatattctc aacattccag tgaccaccca attcagcggc acgctctcgc atggatgtga
     1621 atccaacgcc ggcgtgattg ttcgatggca agccttggcc gttatctgat atttccagca
     1681 gaagagatcc atcttcgaat ttcatattaa tgtcgcaagc gctggcctgc gcatgattga
     1741 tgagattggt aaaggactcc agcacaatac ggtaggcggc cacctcaacc gctgcaggaa
     1801 gcggtggcat cgggtcagtg atgtcaaacg taacctgcaa tccatttgga cctgtatatg
     1861 gagccacgtg ttcacggatg gcagtgacca gcccaaattc atcgagcgcg ggtggacgca
     1921 atgcgtagac caacctgcga atttcggaga cggagcctct cacctgacct ttgagttctt
     1981 ttagcaattc aacgcttgcc tgtggatcga tattcaccat ctccgcggcg gtatcaatgc
     2041 gttgagagag gcttgccagg gtgggtccaa ctccgtcatg caggtcacgg cgtaagcggc
     2101 ggcgttcctc ctcctgcgca gtgacgagtt tttcgcgcga gcgctgcagg tcctgcgtga
     2161 gacgaaggtt ataggcggcg atgcctgcct gcccggcaat gagggtgagc agattcatat
     2221 ccgcagtgga aaataattct ccttccgcgc gcggggcaag gctcaactct ccgacgcgtt
     2281 cactctgata tgtcagcggt agggaaatca attgggtggg tggcttgtta ggtgaggatg
     2341 caaaacgggg cgctggcgtg tcatctgaaa gtgaaatagc cgcatacggt agcctcaatg
     2401 tttgtgccag cgtttccaca atcgtctgca aaacggcatc cggtgccagg gccgagtcga
     2461 gccgccggct gagtcgtgtg aggacagtgg cagggtcgtc acgttctcca tacatcaacc
     2521 gattgaccgc gcgttgaagg cgctcgcgta ggggttcgaa gagaatggca attacgcccg
     2581 tgacaatgaa cgaaacgatg agattcgctc cctgaacata tcgggatgca aaaccaacgg
     2641 caagaatata aagcgcgatg gtgctggcgg agagagcccc ataaacaagc gtccgattga
     2701 tgatggggtt aatatcccac agacggtatt tgatcaccgc caggctgatg gaaattggca
     2761 ggatcaaaat aatgagattg gcagtgatgc cgagagccat cgagtagggc aaaccagttc
     2821 ccagtgggtc ggtattgagc ctgccaagtt ctcgcgtaat caggtagcca actatggcta
     2881 tggtgagacc atatacgacc catttggttt gttgtcgttc gacgatattg gacacccgca
     2941 aataccgata aatttgtgca gcgatgccgc tcagaattcc accggtaaac aggatgccat
     3001 acagattagg attggttttc gcaagattta aaggagagtc ggcgaagtat cggggcagaa
     3061 tctgtaacag ggaccagatc aggagcaggg caactcccca gcggggtgta aaacggccat
     3121 tgggaaagac aaacaggaaa gctaggatgg aaaaactgcc aagcagctca aaaaaattga
     3181 tcagtccatt gagagccggg taaacaagac caatgccgcg tattccgccg gtaaatgtgg
     3241 cggttccgaa gataaccagc atgattgtga cgaagatcgt caatggttca tcgggcttgc
     3301 ggacaaagat gacgatcgca caaactgtat atgtggcagc cacaataata tcaagggcga
     3361 tcgtctgggt caccagatca ttcaccgaca tcccaacatc ttccaaggcc tgcacactcc
     3421 tgagcgacaa ttgatttggt aggcagggct ctacttcgca gacggtaaca cgttgctcgt
     3481 agttgagagg gatgcttatg atgaacattc caatcgccag acagatgctg aggatctgga
     3541 aggcgcgggc gaccatgagc cagcggggag caggagaagc tacggcaaga gttggagaaa
     3601 tcatcatcgc aaggatacct cgttaccttc ccagtgtcac gggagtgtgt tcctgtacag
     3661 actgggaagg ttgtatactg tgagcaactg agttgggcaa ggagaacata tggaccctac
     3721 acaagaagtc ttgaatgtcg aacatgcgtg gacggaagct catctacacg gtgatgtgca
     3781 aaccctggaa gagatcatgg ctttcgatta tgtgaaaatt cagcccgatg gttcgttatc
     3841 ggacaagcag gcgactctcg cttcgtacca acccgatcag cgcgaatggg aggttgcccg
     3901 cggcgacgag tatgatgtgc gtgtgtacgg agatactgcg gttgtcattg gcaggtggac
     3961 ggcgcgtggg attaatcatg gtgagtcttt cgattacgcc gcgcgcttcc tctccattta
     4021 tgtgcgtcgt gacggtcgct ggcagatggt ggcagagcaa tctactgata tcaaatagct
     4081 catcattaat aaaaaatcaa ttggcatata gttgtttcca gcgccctgcg agacttgctg
     4141 caaacaatac aaccgtcagg gcgaaatatg cggcgagcag gatggggttc ggtgacgttt
     4201 gtaatacctg catgaaatcg agtggaaccg cgccgttaac tccgttgaac aaccatagca
     4261 aatagacgac ttcgaagagg cgggaattgt tgctccaggt gcctaacgcc attgccagtg
     4321 atggaacaaa taacacagcc agtaggataa ggggtagacg atccgcacca ccttcgatga
     4381 ccatgcgaac ggttgcgcca ctgattgcga gcgtcgcaag caacacgcct gaaaaccagg
     4441 atgccatgaa ttggtgccca agtggatggg cggaggagta cgtcagttta tatgtctcgt
     4501 aaaaggtttc gcgcattccg agcgcggacc aggcgatgac gggccagatc atggccagcg
     4561 ccagcgtcac atctgagcca ccgggtggac ctatcagccc aagcagggaa atgaacgcgg
     4621 cgaccaggta ccaccaccag ggcttacctt tcaccagcaa tttgaactca gcgttgagca
     4681 ggctcaaaaa acgcgaacga gaatttcgat gattgatcgg cgtcaagttc acaggtttgg
     4741 actccgcaaa cgccggctca gccaagggtt gtctgcgcgg tcggtttgga agaagagatt
     4801 tcaacactga cgtaccatct acagatgtgc gcgcagggtc gaagcgatca aacggaaacg
     4861 aagcggcgag cgcgagaagc cctgctatac caaaccatgc caatcgtccg agcatgatcg
     4921 tcagtgacca ttgaatgcct gcaaaggtca ccacctgagg aacgactcca aattccgcgc
     4981 ctccaaagtt aatatgtccg ctgaactgtg gggctacctg ctgccccacg ctttgcaatg
     5041 cagccagaag acctgtaacg cccaacggat caagccacgg ttcaacaact ccgatgttgt
     5101 aacggaagat ccctggaacc gcgaggttgt ccatgaagat gtaccagccg agaaaatata
     5161 tgacattgcc cgcgctgccg ctcaggaagg ggatactctc gaagagcaca gccatcgctg
     5221 ctgtcagcgc gatgatcggg aaccccatca accagatcgg tgccgagagc gcccaataat
     5281 caatcggaaa gatttcgccg cgcaaaagct gcattacgat tgccatcaga tttaaaacaa
     5341 ccagcatagc ggtcagcaca gccatgttgc tcagccattt gcccagggta tagatcggtt
     5401 tgctcatcgg agtagtggca aggatctgac ccacgcggga acgacggtcg cgctcgatcg
     5461 tgttcttggt cagaaagaag ccaagcacag gcatggagat gacgatcaga atcccaaaca
     5521 ccgaaccaat ccaggcggag ttatacgcgc cgcgccatgg tcccagtgcg agcatcaagg
     5581 tgcttgaatc ggcaggcggg atgtaaatgt agccaatgag gacagccagc ccaagtgtta
     5641 cgaaaaaact actccggcgc gtgcgctcga gaaaatccgc gcgcagcatg tgatagatca
     5701 aattcagggt cttcataaac ccttctttcc atggttgcca ttggagatca atttgaggta
     5761 ggcgtcttca aaggtcggtg tgacggggcg cgcatcttgg gcgggcgcag cgcgcgagat
     5821 gactcgcatc tgcattccgt ccgtccggcg cagtgtgccg ctgaccagat gcctgctttt
     5881 gacttctgcg aattcagagg ttgggacgat ccattcccag acctttccct ctaccacgcg
     5941 cagtaattct tcggaattgg tgtccatgat gaggcgtccg tgattggcga tgacaatgcg
     6001 ggtggcagtc gcttccacgt ccgaaacgat gtgcgtggag aggataatga ttcgttcgcc
     6061 cgccaggtcc gcaatcagat tccgaaagcg gacgcgttcc tcagggtcca gccccactgt
     6121 tggttcatcc acaataagca attgcggatc gttgagcaaa gcctgggcaa tgccaatacg
     6181 ttgtttcatg ccgcccgaat atccgcccag tggtcgcttg cggacctccg tcagattcac
     6241 cagttgcagg agttcgtcaa tgcggcggcg ggccgtgcgt ccatccagcc ccttgaccgc
     6301 tgacatgtac tctaaaaatt cctgcgcgct caggtttgga taaatcccaa aggactgagg
     6361 cagataaccg aggatttgac gcagttcatc tggcctgcgt acgatatcta cgccgttcca
     6421 ggagaccgcg ccttgcgtgg gacgcgtgat ggttgccagg atattcatca aggttgactt
     6481 ccctgcgcca ttgggtccaa gtaaacctaa aatgccaggt ttgagatgga ggctgaattc
     6541 tttgagaccc caaacctccc ctgaatattt tttgccgacg tggttgatga gtagttccat
     6601 aatcaatttc ctttctgaat ggcgacagga taaattatcg gtttcctgct gtcacgagaa
     6661 tagccacata gtttgcctgg gaaattttcc cgatcaggtt gtgagtctgt cctcatgcga
     6721 gagggaaagt atcgttgtat cctgcggctg caacttgaag aaaggctgtt caacgacgca
     6781 tagattgatc tgtggaattg atttcacaag acaggagatt tgcgcaatgg cttcagaaac
     6841 gtacgttatc gaaacacagg gtttgaccaa gtcctacaaa ggagtaaacg ccttacaacc
     6901 tttgaacctg cgggtccgtc aacattccat ttgtggattt ctgggtccga atggtgccgg
     6961 caaaagtacc accatcaaac ttctgttggg gcttgcgcgt cccacgggtg ggagcgcaaa
     7021 ggtgtttgga atggacatcg tccgcgatag cacagacatc cgaaagcggg tgggatatct
     7081 cgcgcaggac ccgcgttact atgaccatat gacagcgcgc gaaacgctgc gctttacagc
     7141 ccgatttttt tattccggcc ctgaaaagcg aatggaagac cgcatcgacg aaaccctcga
     7201 gctggttggg ctttctgata aagcggatcg ctcgatcaaa ggcttctcgg gtggcgagcg
     7261 tcagcgattg ggaattgccc aggcgcagat caattaccct gatctactga ttttggatga
     7321 acctgccgca tctctggacc cgatgggtcg ccgcgacgtg ctggaggtga tggaacgtct
     7381 tcgcaagcat acgaccatct tctattcgac acatatcctg gaggatgttc agcgggtgag
     7441 tgataccgtc gccattttga atcatggtcg tctcatcgca caggcgccga tcgccgaact
     7501 gacatccggc aaggatggca tcatctactc tttgaaggtt aagggagatg gg
//
LOCUS       DASTJP010000550         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1133721, whole genome shotgun sequence.
ACCESSION   DASTJP010000550 DASTJP010000000
VERSION     DASTJP010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133721"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..747)
                     /locus_tag="VFQ23_16065"
     CDS             complement(148..747)
                     /locus_tag="VFQ23_16065"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HET9908168.1"
                     /translation="MDLKPFLDIASSDHKHVCPRQILGVRFGLRGMEILGFEVSLRHK
                     HFLVISETDGCFIDGVIAVTRCTVGHRRLRVQDYGKVAATFVNLKTGQAVRIAPTLDV
                     REKAQRYAPEENRHYFAQMKAYQIMPDEELMSIQEVTLNFSLDELISHSGIRVNCAGC
                     GEEIINEREVMIEGKNYCKSCAGGGYYQSIKTNINHHTV"
     gene            886..2574
                     /locus_tag="VFQ23_16070"
     CDS             886..2574
                     /locus_tag="VFQ23_16070"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET9908169.1"
                     /translation="MTQSMDREALDRQLVEEWADKPAPLLGVLHAFHDRDGHISEAAL
                     RAISKGLRQPLADLFGTVTFYHHMSREAEGLNKPRVCTGPVCSLQGGNECLSALADKG
                     ATPMPCAGRCDEPVPVLIGHEQFVGDAEGNLKRAPTLLPPANPGGVEECVFAKIREPE
                     RNTLAGYQRTGGYEGLMLAVKEMAPEGVIEHVKASKLAGRGGAAFPTGQKWDAVAKAS
                     GTPKSIVCNADEGEPGCFKDRAIMDYDPFAMIEGMTLAAYATGATRGFIYLRYEYPET
                     AFVLEKAIADAYSANLLGKNILGANFDFDLYVRRGGGAYICGEEGSLLNSLEGKHPFP
                     RNRPPFPVTHGFENLPTAVNNVETLAAVAQIMRHEPEWYVNLGVNGQPGTKVVSLSGD
                     IQRPGNYEVPFGLPLRTLIYDWAGGPRPGRAIQAVTMAGLSGGFLSHDDIESATVDEP
                     SIRSKGSMLGAAGMIVYDDSRDMVEAAHNAMEFFAHESCGKCFPCRIGTQRLVERLNG
                     DGPGDMNIWLDEVKDLNETMKSVSACGLGMAAPLVVESLIKHFPEQVKAHVETR"
     gene            2603..3259
                     /locus_tag="VFQ23_16075"
     CDS             2603..3259
                     /locus_tag="VFQ23_16075"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9908170.1"
                     /translation="MSVNPTIILNGKEVSFTPGETIYEVAARQDTFIPTLCYDPRLKS
                     FGACRLCVVDIKGIKNPVASCTTAASPGMDVTTNNERIDKFRKTLLEMVVSENRELEV
                     TPLRSIASQELTILVEKYDASHGRFQGAHSGHSKLDDKNPFLLRDYDLCISCYRCVRV
                     CAEQEGDHAISVMNRGFHTQITTEFNGFLRDSACTFCGQCVQTCPTGALGDKKALRNA
                     "
     gene            3386..>5085
                     /gene="fdhF"
                     /locus_tag="VFQ23_16080"
     CDS             3386..>5085
                     /gene="fdhF"
                     /locus_tag="VFQ23_16080"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3827..3829,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HET9908171.1"
                     /translation="MQDIRELPLINIGVEKTRTICGYCGVGCSVDILTKDNRIIGIQP
                     AMDGPANLGALCVKGQFAFDYVHHPDRLKKPLVRREDGKLHETSWDEAIQVAADGFRK
                     AAQKYGREAIYGVASGRTPTEADYLMQKFIRAGFGTHNIDNCSRAUHAPTVAGLATTL
                     GRGAMSNPLSDLEKADVYFCIGTNMTEAHPVAATRLKRALARGSKLIVADPRYIKLAE
                     LAHLYLPLRVGTDVALLLGMAHVIVRENLIDHKFVAERTTGIEEYLEHVKKFTPEWAS
                     EITGVPAKDIETAAIMYGAADKASIYYTLGITEHICGVDNVQSLSNLALMTGHLGREG
                     CGINPLRGQNNIQGAGDAGAAPGDYPGYQRVNIPENRDKFQKLYGRELDPDRGITKVT
                     ALERCGDGIHAMLIDGENTLISDPDREHTEHALKKLDHLVVIDIFLTETAEMADVVLP
                     ATSWGETDGTLTNTERRIQRVRAAVPPVGEAKPDWWILSSLANALSIPGFEYDSPKEV
                     FNELCSVAPIYAGVDWDLIDDGQYQWPVPYKGHPGTPRLHEGGFISGKALFKVIGYRD
                     P"
BASE COUNT         1284 a         1210 c         1344 g         1247 t
ORIGIN      
        1 taaccatggc tctgccctgc aattaaaaac cccaacaagg cgaattcggg cgatatattc
       61 ccggagtgct tattttcact catatatact cactgagtga gtatatatgc agtttaaaat
      121 cctgacaagg gaaatttttg ctgcctttca aaccgtgtgg tgattgatat tggttttgat
      181 gctttgataa tagccacccc ctgcacagga cttgcagtaa ttttttcctt caatcataac
      241 ttcacgttcg ttgatgattt cttctccgca acctgcgcaa ttaactcgaa tacctgaatg
      301 tgaaatcaat tcatccaacg agaaattcaa ggtcacttcc tggatgctca tcaattcttc
      361 gtctggcata atctgatatg ccttcatctg agcaaaataa tgacggttct cttcaggagc
      421 atagcgctgg gccttttcac gcacgtccag tgtcggagca attcgaactg cctggcctgt
      481 ttttagattg acgaaggtcg ccgctacctt tccataatcc tggactctca atctgcgatg
      541 tccaaccgtg catctcgtca cggcgatgac tccgtctatg aaacagccgt cggtttccga
      601 gatgacgaga aaatgtttgt gacgcagaga cacttcaaac cccagaattt ccattcccct
      661 aagcccaaag cgtacaccca ggatctgcct cgggcagacg tgtttgtggt cactcgatgc
      721 gatgtcgaga aagggtttga ggtccatatg tgagtcatta taaatccgat tgcattcgca
      781 caaggtaacc tcacagggtg acaaatgaca cccatataat tgacaattgt cacatacctg
      841 cctattctcc ttgtaaaaac tctgaaacta ccgtagaata tttttatgac acaatccatg
      901 gatcgggaag ccctggatag acaacttgtg gaagaatggg cagacaaacc tgccccactt
      961 ttgggggtat tgcacgcctt tcatgatcgc gatgggcaca tttcagaagc tgcgctgcgg
     1021 gccatctcaa aaggattgcg ccagccgctt gctgatttat ttggcaccgt cactttttat
     1081 catcacatga gccgtgaagc agaggggttg aacaaacccc gcgtctgcac tggacctgtg
     1141 tgcagtttac aaggcgggaa cgaatgtctt tcggctttgg cagacaaagg cgcgactcct
     1201 atgccgtgtg caggtcggtg tgatgaaccg gtgcctgtgt tgatcggtca tgaacagttt
     1261 gtcggcgatg cagaaggaaa tctaaagcgt gcgccgactc ttttacctcc tgcgaatcct
     1321 ggcggcgttg aagaatgcgt gtttgcgaag atccgcgagc ctgagcgtaa tacattggcc
     1381 gggtatcagc gaaccggtgg ttatgaaggg ctaatgctgg cggttaaaga aatggctcct
     1441 gaaggtgtca ttgaacacgt taaggcgagc aagctcgccg ggcgcggggg agcggcgttt
     1501 ccaaccggtc aaaagtggga tgcggtggca aaggcatctg gaactccgaa gtcgatcgta
     1561 tgtaatgctg atgaagggga gccgggctgt ttcaaagatc gcgccatcat ggactacgat
     1621 ccgtttgcga tgatcgaggg gatgacgctg gcggcatacg caacgggcgc gacacgtggc
     1681 tttatttatt tacgttatga atatcctgag acagctttcg tcctggaaaa ggcaattgcg
     1741 gatgcctatt ctgcaaattt gcttggaaaa aatattttag gcgcgaattt cgattttgat
     1801 ttatatgtgc ggcgtggtgg cggtgcatat atctgcggcg aggaaggctc attgctcaat
     1861 agtctcgagg gcaagcatcc gtttccgcgc aatcgcccgc cgttccccgt cacacatgga
     1921 tttgaaaatt taccaacggc agtgaataac gttgaaacgc tggcggctgt ggcgcaaatc
     1981 atgcggcacg agcctgaatg gtatgtcaat cttggggtga acggtcaacc cggaacaaaa
     2041 gtggtttcgc tctccggaga tattcaacgg cccggcaatt acgaagtccc gttcgggctt
     2101 ccgcttcgga ccttgatcta cgactgggcg ggtggaccca ggccaggtcg cgccatccag
     2161 gcagtgacga tggctggact ctccggcgga tttttatctc acgatgatat tgaatccgca
     2221 accgtggatg aaccgagtat ccgcagtaaa ggctccatgc tgggagcggc aggcatgatt
     2281 gtgtacgacg atagccgcga tatggttgaa gcggcacaca acgcgatgga attctttgca
     2341 catgaatctt gtgggaagtg ttttccttgt cgcatcggca cgcaacgtct ggttgagcgg
     2401 ctgaatggcg acggtccagg agatatgaat atctggcttg acgaagtaaa agatttgaac
     2461 gagaccatga aatccgtcag cgcctgtggt cttggcatgg cggcgccgtt ggtcgttgaa
     2521 agtctgatca agcattttcc ggagcaagtg aaagcccacg tagaaacaag gtgacagtta
     2581 actttggaaa aagagatagt ttatgagcgt taacccaacg atcattttga atggcaaaga
     2641 agtgtcgttc acacctggcg aaacgattta tgaagttgca gctcgtcagg atacttttat
     2701 tccaaccctg tgctatgacc cacgactgaa atcgtttggc gcatgtcgct tatgtgtggt
     2761 cgatataaaa ggtataaaaa atccggtggc ttcctgtacg actgccgcaa gcccgggcat
     2821 ggatgtgacg accaacaacg aacgcattga taagttccgc aagacgcttt tggaaatggt
     2881 cgtttcagag aatcgtgagt tggaagtcac acctttgaga agtatcgctt ctcaggaatt
     2941 gacaatcctt gtcgaaaaat acgacgcaag tcacggacgg ttccagggcg cgcactcagg
     3001 tcacagcaaa ctggatgata agaatccctt ccttttacgg gattacgatt tgtgtatttc
     3061 gtgttatcgt tgtgtacgtg tgtgcgctga gcaggaaggc gaccacgcga ttagtgtgat
     3121 gaaccgtggt ttccatacgc agatcaccac ggaattcaac ggctttctca gagattcagc
     3181 ctgtaccttc tgcggtcagt gtgtgcagac ttgccccact ggcgcgttgg gggataaaaa
     3241 ggccttgaga aacgcttaag atctcaaaca caaaggacac tagggaaaga ccttaagact
     3301 ttccgctttg tgcactctgt gccgttcgta ttaaaaaatg tctccagctt aatctggcgg
     3361 caggaaagga gtagcgaaca tgacaatgca ggatattcgt gagttaccgc ttatcaatat
     3421 cggcgtggag aaaacgcgca cgatctgtgg ttattgtggc gtcggttgtt cggtggatat
     3481 tctcaccaaa gacaaccgta tcatcggcat tcaacctgcg atggatggac cggccaatct
     3541 cggcgcgttg tgcgtgaagg gacaattcgc gtttgattat gtccatcacc ccgaccgctt
     3601 aaagaaaccg ctcgtccgcc gggaagacgg aaaactgcac gagaccagct gggatgaagc
     3661 cattcaagtc gcagcggatg gcttccgtaa agccgcgcaa aagtatgggc gtgaagccat
     3721 ctatggcgtc gccagcggtc ggactcccac cgaagccgat tacctgatgc agaaatttat
     3781 tcgcgcaggc ttcggcacgc acaacataga caattgttcg cgtgcctgac acgctcccac
     3841 tgtcgccggt ctggcgacaa ccctgggacg cggtgccatg tcaaatccac tatcggacct
     3901 ggaaaaggcg gatgtgtatt tctgcatcgg cacaaacatg accgaagctc accccgtagc
     3961 ggcaactcga ttaaagagag ccctcgcacg cgggtcgaaa cttatcgtcg ccgacccgcg
     4021 ttacatcaag ctggcggagt tagcacactt atatctacct ctccgcgtcg gtacggatgt
     4081 cgctttactc ctcggtatgg cgcatgtgat cgtgcgcgaa aacctaattg accataaatt
     4141 cgtcgcggaa cgtaccacag gcattgaaga atatcttgaa catgttaaga agttcacccc
     4201 cgaatgggca tctgagataa ccggagttcc ggcaaaagat atcgaaacag ctgccatcat
     4261 gtatggagct gcagataaag cctctatcta ttacacactc ggaatcacag aacacatttg
     4321 tggcgtggat aacgtccaaa gcttgagcaa tctggcgttg atgaccgggc atctcggtcg
     4381 agaaggatgt gggatcaatc cactacgcgg acaaaacaac attcaaggcg caggtgatgc
     4441 tggcgcggct ccgggtgatt atcctggtta tcaacgagtt aacataccag agaatcgtga
     4501 caagttccaa aaactatatg gacgtgaatt ggaccctgat cgcggcatta caaaagtcac
     4561 tgcgttagag agatgtggcg atggtattca tgccatgttg atcgatggcg aaaacacatt
     4621 gatttctgat cctgatcgtg aacatactga acacgcattg aaaaagctgg atcatctggt
     4681 ggtgatcgat atcttcctta ccgaaacagc cgaaatggca gatgtggttt tgcctgccac
     4741 atcctggggt gaaaccgatg gaacgctaac caatacagaa cgtcgcatcc agcgtgtgcg
     4801 ggctgcggtc cctccggttg gagaagccaa gcccgattgg tggattctca gttcgttggc
     4861 aaatgcattg agcattcccg gctttgaata tgattcacca aaggaagttt tcaacgagtt
     4921 gtgtagcgtt gcgccgattt atgcaggtgt ggattgggat ttgattgatg atggtcaata
     4981 tcaatggcct gtcccttaca aggggcaccc gggaacgccg cgtttgcatg agggcggttt
     5041 catcagcggc aaggcgctct tcaaagttat tggctaccga gaccc
//
LOCUS       DASTJP010000551         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1249753, whole genome shotgun sequence.
ACCESSION   DASTJP010000551 DASTJP010000000
VERSION     DASTJP010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249753"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VFQ23_16085"
     CDS             complement(<1..1195)
                     /locus_tag="VFQ23_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9908172.1"
                     /translation="MYKQSNIRRWLAGIGNAFLILVMLVSAMGLQATPVLAAPAGTAL
                     QFNGTSQYVTFGNTRLIPGTLTNSPTWNTQTNSRLGRSSLLLNGTNQYVTTGIGTTFN
                     SASFTVETWFYRTAAGVGVTTGGSGIASAIPLLTKGTSEGETSTADINYFLGIDATTA
                     KLVADFEEGAGGGSPSLNHPITGTTTIALNTWYHAAATYDGNKWQLFLNGNLEGELVI
                     DRPANAAVISPLALGTSIMSNGTTTQGFFAGRLDEARVWNYPRTQADIQASMNSEILT
                     PTTGLIGRWGLNDASGSTASNLNRLGLTSFTLEAWVKRDTGGATMSTGTNGFDGTGGR
                     PFIYPVLTKGMGEGESPANLNTNYFLGITANGFVGADFEDTAGGGNRPAWGTTPIPVG
                     EWHHIA"
     gene            1782..2675
                     /locus_tag="VFQ23_16090"
     CDS             1782..2675
                     /locus_tag="VFQ23_16090"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET9908173.1"
                     /translation="MPENQLTLHLTPNSTLTSAINSWEAYLTDKGRSPNTIKAFLSDV
                     RLLTSFLPEDVTLSRITTKDLNRYFEWMENEREVPCSPKTLARRITATKSLFRWLHQY
                     GVLVVDPAEKVAQRSAISPLPIVLTRDEYEAVLLAADRHRRGAKPDARSYALVYLLLT
                     TGIKKSETLGLKLEHVDLETPNGPQIFIRYASPANRYKERKLTLPDDWVTAYNEYLSQ
                     YHPTEQVFPWSPRRLEYLLEDIGNEAGLTKHLSFDMCRWTCALNDYRAGEEPDKIRQK
                     LGVSKIQWRELHNKLKQLSGS"
BASE COUNT          683 a          765 c          670 g          650 t
ORIGIN      
        1 cagcgatatg gtgccactct cccaccggaa tcggtgttgt tccccaggct gggcggttgc
       61 caccgccagc cgtgtcttcg aaatccgccc caacaaaacc gtttgctgtg atcccgagga
      121 aataattcgt atttagatta gccgggcttt cgccttcccc catacctttt gttagcactg
      181 ggtaaatgaa gggtcgacct cctgtgccat caaaaccatt tgtgcccgta ctcatagttg
      241 cgcccccggt atcacgcttc acccaggctt cgagcgtgaa agaagtcaga cctaaacggt
      301 taaggttgct ggcagtgcta cctgaggcat cattcaaccc ccagcggccg atcaatccgg
      361 tagtgggggt taaaatttca gaattcatgc tcgcctggat atccgcctgg gtacgcgggt
      421 aattccaaac acgcgcttca tccaatctgc ccgcgaagaa tccctgggtg gtggttccat
      481 ttgacataat ggaggttccg agagccagag gtgagatgac agccgcattg gccggtctgt
      541 caattacaag ttccccttcc aggttcccat ttaagaaaag ttgccactta tttccatcat
      601 aggtggctgc agcgtggtac caggtattca gggcaatcgt agttgttcca gtgattggat
      661 gatttaagct gggcgatcca cccccggcac cttcttcaaa atctgcaacc agtttcgcag
      721 tcgtcgcgtc aattcctaaa aagtaattga tatctgcagt agaggtctca ccttccgacg
      781 tgcccttggt caataatgga attgcagacg caataccact tcctcccgtg gtaacgccaa
      841 ctccagctgc agtccggtag aaccaggttt caaccgtaaa agaggcagaa ttaaaagttg
      901 tgccgatccc cgtcgtcaca tactggtttg ttccattcaa tagcaaggag gatctgccta
      961 atcgggagtt agtctgtgta ttccaagtag ggctgtttgt cagcgttcct ggaattagtc
     1021 ttgtatttcc aaacgtcaca tactgactcg tcccattaaa ctgtaaagcc gtcccagcgg
     1081 gggcggctaa aacaggggtc gcctgcagac ccatggctga gacaagcatc acaaggatca
     1141 ggaacgcgtt cccaatacct gcgagccatc tgcgtatatt cgattgtttg tacatatatc
     1201 ctcctcaaac acgcccctga aataaacaaa atccatgcca gaaacctatt ccagcatagt
     1261 ttgcatgcta tttcacctta caaaaatgca aatctggtgt caaataacat ttgtcatcta
     1321 agtagtgata tttatcatcc cggggttacg gataacaaac aatacattcc accctaggat
     1381 tccgtgccga gagaggcctc cggcataggt gaattcggtc gataattgtt ttggctcgat
     1441 tcgtgttgga tgcccccgtg gtgacaataa gcaaacaggc cagctgagct ggactctccc
     1501 gatgaactag cggcatattg atccctttgc ctggagatga actctccaca atcacaaaga
     1561 tttcacccct tctgagacct ttgtactatg tgcacccgtt ggatttgttc attatatcga
     1621 tttcctaccg aaaaacaaga taaaactagt catattaagg ttgcactttc gatgcattga
     1681 tacggaaggg agcagattga ccaggcactc attcattagg gtgttgacat cccccccttt
     1741 tttcgctaaa ctatacgaaa gaagagaaca caggagctgt catgcctgag aaccaactga
     1801 ctttacatct gaccccgaat tccaccctga cttccgcgat caattcctgg gaagcatacc
     1861 tgacggataa gggacgttcg cccaatacga tcaaggcctt cctttcggac gtgcgcctgc
     1921 tgaccagttt cctcccggaa gatgtcactc tgagcaggat caccacgaaa gacctcaacc
     1981 gttacttcga atggatggaa aacgaacgcg aggttccctg cagtccaaag acgctcgccc
     2041 gccgcatcac cgcgaccaag tcgctgtttc gctggctgca ccagtacggc gtcctggtcg
     2101 tggaccctgc cgagaaggtt gcgcagcgct cggcgatcag tcccctcccg attgtactga
     2161 cccgggatga atatgaagcg gtcctgctgg cggccgaccg tcaccgccgt ggcgcgaaac
     2221 cggatgcgcg ttcctatgcg ctggtctatc ttttgttgac gaccgggatc aagaagagcg
     2281 aaacgctggg gctcaaactg gaacacgtcg acctggaaac accaaatggc ccgcagatct
     2341 tcattcgcta tgccagtcct gcaaatcgtt ataaggagag aaagctgacc ctgccggatg
     2401 actgggttac cgcctacaac gaatatctaa gtcaatacca tcccacggag caggtcttcc
     2461 cctggtcacc gcggcggctg gaatatttgc tcgaagatat cgggaacgag gccggtctga
     2521 ccaagcacct gtccttcgac atgtgccgct ggacctgcgc cctgaacgat taccgcgccg
     2581 gcgaagaacc cgacaagatc cgccagaaac tcggcgtctc caagatccag tggcgcgagc
     2641 tgcataataa gttgaaacag ttgagtggga gttaaccttc cgcgctgacc gaagggggcg
     2701 caggttgccc tgcggagcga atgccattcg agcgaagtcc aagcgccgtc acaagaaatc
     2761 tcctcatt
//
LOCUS       DASTJP010000552         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_750374, whole genome shotgun sequence.
ACCESSION   DASTJP010000552 DASTJP010000000
VERSION     DASTJP010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750374"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..537)
                     /locus_tag="VFQ23_16095"
     CDS             complement(115..537)
                     /locus_tag="VFQ23_16095"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET9908174.1"
                     /translation="MNKRLSQLTIALVILGLLVSIYMTIFKITSNENMCIGSKDCSIV
                     NASRYSEVNNIPVAVIGVVGYSTLLAILLLERRPGFIRQNGTLLFFALAAIGFFFTLY
                     LIFVEIVLIKAYCPFCITSQVAMTFIFILSVIRLVKQP"
     gene            complement(544..1767)
                     /gene="glp"
                     /locus_tag="VFQ23_16100"
     CDS             complement(544..1767)
                     /gene="glp"
                     /locus_tag="VFQ23_16100"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET9908175.1"
                     /translation="MALLSVDDARARILSHFQAVTTETLPLVDCNHRVLAQDVAATTD
                     LPPFDNSSMDGFAIRVADLNADPLQRLHVIADIPAGVSPTISLSQGEAARIMTGAQVP
                     AGADAVVPVEDTDFNNRDAGSAPPELVVINAPVNKGANIRARGMDIHAGEIVLSKGRA
                     LKPQDIGLLAMLGYAEVPVHTKVRVGLFSSGNELLEVGAPLEPGKIRDSNSYTLEALL
                     KEAGADVIRLGVAKDDRESVKALLDKAVESRVDLILSSAGVSVGAFDFVKEVIEANGK
                     LDFWRVNMRPGKPLAFGAYKNIPFIGLPGNPVSAFVGFEVFVRDVIGKLNGIYNGSRP
                     KVQVRCAEQIDSDGRESYLRAEVREENGSFVAHLTGHQGSGNLHSLVQANALLIIPAG
                     VKCVPAGQEVNAWLI"
     gene            complement(1830..>2036)
                     /locus_tag="VFQ23_16105"
     CDS             complement(1830..>2036)
                     /locus_tag="VFQ23_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9908176.1"
                     /translation="DRQGLMGDISTLLQNEGVNIADVSVNFNRSVADLKLVVEVRDLA
                     QLSRVLTRIESLPNVVEAQRTKPG"
BASE COUNT          541 a          522 c          477 g          496 t
ORIGIN      
        1 tggtgcaacc ggcatccgga tcgacctcaa ccgcagccgc gctgcaatac ccttcgtcta
       61 aaaagacaca atccaagtaa tgacatttaa ttcggggcat ttctcctcct ggaattaagg
      121 ttgtttgacc aggcggatga ccgaaagaat aaatatgaaa gtcatcgcaa cctgagatgt
      181 aatacaaaac ggacaatagg cttttatcag gacgatttcg acaaaaatga gatacagcgt
      241 aaagaaaaac ccgatggctg caagtgcaaa gaacagcagg gttccatttt gtctaatgaa
      301 gcccggtctt cgctcaagca ggagaattgc caacaacgtt gagtagccga cgactccgat
      361 tactgcaaca ggaatattgt ttacttctga ataccgactg gcattcacaa tcgagcagtc
      421 ttttgaccct atgcacatgt tctcgttgga ggtaattttg aaaatggtca tgtagataga
      481 gaccagtaaa cctagaataa cgagtgcaat tgtgagctgt gaaagtcgtt tattcatccg
      541 tcctcatatc aaccaagcgt ttacttcctg accagcaggc acgcatttta caccagcggg
      601 gataataagt aaagcattcg cctgtaccaa agagtgtaaa tttccagagc cctgatggcc
      661 cgtaaggtga gcgacaaaac tgccattttc ctcccggact tctgcccgca gataactctc
      721 acgaccatct gagtcgattt gttcagcgca tcttacctga actttgggcc ggcttccgtt
      781 ataaattccg tttaattttc cgatcacgtc gcgtacgaag acctcaaacc ccacaaaggc
      841 cgaaacggga ttccccggca gcccaatgaa tggaatattt ttataggctc cgaaagccaa
      901 aggtttacca ggtctcatgt taacgcgcca aaagtctaat ttgccgttgg cttcgatcac
      961 ctcttttaca aagtcaaacg caccgacact tacgccagcc gaggaaagga tcaaatcgac
     1021 tctggattca actgccttat cgagcaaagc tttgacggac tcgcgatcat cttttgctac
     1081 gccaagccga attacatcgg cacctgcttc tttaagtaat gcttcaagcg tatatgaatt
     1141 cgagtcgcgg attttgcctg gctccaatgg ggcgccgact tcgagcaatt cattgcctga
     1201 tgaaaaaagg cccacgcgaa ctttcgtgtg tacgggcacc tcggcgtatc caagcattgc
     1261 cagcagtccg atgtcttgcg gcttgagggc acgcccttta gaaagcacaa tttcaccagc
     1321 gtgaatatcc atgcctcggg cgcgtatatt agctccttta ttcactggag cgttgattac
     1381 caccagctct ggaggtgcag accctgcatc acggttattg aaatccgtat cttccaccgg
     1441 gacgactgca tctgcacctg ccggtacctg cgcaccggtc ataatgcgtg cagcttctcc
     1501 ctgtgaaaga gaaattgtcg gcgaaacgcc ggccgggata tcggcgatca cgtgcaggcg
     1561 ttgtagtgga tcagcgttaa gatctgcaac gcgaatagcg aacccgtcca ttgatgagtt
     1621 atcaaaaggt ggaaggtcag ttgtggcggc gacgtcttgc gcgagaacgc gatggttaca
     1681 atccaccaaa gggagtgttt ctgttgtaac cgcctgaaag tgagacagga tgcgggcgcg
     1741 cgcatcatcg acggagagta atgccataat cttaaagcgg cgacattata ccccttccgc
     1801 cgctaaaacg gttcttcaaa ttgtgtgagt taaccgggtt ttgtgcgttg agcttcgacc
     1861 acgtttggta agctctcaat gcgtgtcagt actcggctca attgagccag atcacgaact
     1921 tccaccacca gtttcaaatc ggcgacacta cgattgaaat ttacagagac gtccgcgatg
     1981 ttcacgccct cgttctgaag caacgtggaa atatctccca ttaacccctg ccggtc
//
LOCUS       DASTJP010000553         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_993934, whole genome shotgun sequence.
ACCESSION   DASTJP010000553 DASTJP010000000
VERSION     DASTJP010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993934"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="VFQ23_16110"
     CDS             <1..1637
                     /locus_tag="VFQ23_16110"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9908177.1"
                     /translation="LTLNVESPQPQLLPPVLVLGIALLTNLIVSLSSRKLNQLVIHHP
                     TLISVDLFFSAGILALSGGAHSPYYLYALSPLLAGAFFFQMSGALITSMIFTPLYLIG
                     SHLNSQLTLGHTDLIALTTQLAGIWLIPILFAYSSVLLKDINQAREELTSARDELAQK
                     HENLAMAHRQLEIIHELTVLLQAAPDLITVQERVLGAVTTDLGFSKAVVALVDPAREE
                     LGGWLVYPAEETFPHTHALPLKSDNGMVIKALLNRQSFISSGEILIQDPQLNNWLDQN
                     NWFVLPLYLREHPVGVLLVDQSGLTHERENTLQTVANQAALALGTTILCIDRARRLAV
                     ETERNRIARDIHDTVAQSLFGIVYSLDACITMLPQKADDVKNELIELRSLANSAHDEV
                     RRSIFDLWPSALTIELFMADLTNYVGSCCRPHSFNIIFTNQGDFNSLSAGLRRTVYRM
                     AQEALANAARHSGAQSARLCLTVTADEVYLAVSDEGNGFDPAMALSRSQNREHFGLQG
                     IQERARAMGGDCEIVSQPDAGTHIMINLPIKVPHHA"
     gene            1630..2280
                     /locus_tag="VFQ23_16115"
     CDS             1630..2280
                     /locus_tag="VFQ23_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908178.1"
                     /translation="MPEAIRLLIVDDHAVVRKGLAMVLRLEPDIEVVGEAGNGRIGLD
                     AARSLNPDIVLVDLVMPEMDGQEMALALRRSHPKIKIMMLTGTEVDDRIFDLVAVGIE
                     GYVLKNIEPGELVRAIHAVVHGEAYLHPDVMKKVLSHMHSQAQQPTPSVSLTPRELEV
                     LEWMATPNTYRQIAKQLTVGEETIRSHAKNILDKLKQPNRAQAVLAAMKMGLLEIK"
     gene            complement(2601..2672)
                     /locus_tag="VFQ23_16120"
     tRNA            complement(2601..2672)
                     /locus_tag="VFQ23_16120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2638..2640),aa:Met,seq:cat)
     gene            2928..>3583
                     /locus_tag="VFQ23_16125"
     CDS             2928..>3583
                     /locus_tag="VFQ23_16125"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908179.1"
                     /translation="MKKTTLIVIEGRHAEIPSFANDLQKKGFDIRSFQNGGQAASKLK
                     QINPAVAVINAASLRSTGIRICLSLRKKDAKLPIILILEKTRPVDKELADTVLALPFT
                     VQKLVNRIKALMPGDGNNVISVGPIRLDVEHRRVKCLGKSTKLTPRLITLLSLLMDKH
                     GEVVEREALFKKVWETNYTGDTRTLDVHISWLRRAIELDPDNPKFLKTIRGVGYRLDV
                     "
BASE COUNT          873 a          992 c          863 g          855 t
ORIGIN      
        1 tcctgacttt gaatgtcgag agcccgcagc ctcaactcct gccccctgtt ctggtactgg
       61 gtattgcgct cctcaccaac ctgattgtct ccctgtccag tcgtaaactt aaccaactgg
      121 tcattcatca ccccacactc attagtgtcg acctcttttt ttccgcaggc attctcgcgc
      181 tcagtggtgg tgcccatagt ccctattatt tatatgcact aagtccattg ctggcgggag
      241 catttttctt tcagatgtcc ggggctttga tcacctccat gatcttcaca ccgctgtatt
      301 taattggcag ccatttgaat tcacagctca ctctggggca cactgacctc attgcattga
      361 ccactcaact cgccggcatc tggttgatcc caattctttt tgcctattca tccgtcctat
      421 taaaggatat taaccaggcc cgcgaagaac tgacctcagc gcgtgatgaa cttgcccaga
      481 aacacgaaaa tcttgccatg gcgcaccgtc agctggagat catccatgag cttacggtgc
      541 tgctacaggc cgcgcccgat ctcattaccg tgcaggaacg cgtgcttggc gcagtcacaa
      601 ccgatctggg tttttcaaaa gccgtcgttg ccctggttga ccctgcccgt gaagaacttg
      661 gcggctggct ggtttacccg gctgaagaaa cctttcccca tacccacgcg cttccgctaa
      721 aatccgataa tggcatggtc attaaggctt tgctcaaccg ccagagcttt attagtagtg
      781 gcgaaatcct tatccaggat ccacaactca acaactggct cgatcagaat aattggtttg
      841 tgcttccgct ctatttgagg gaacaccccg tcggcgtttt gctcgtcgac caatctggac
      901 tcacccacga gcgtgagaat accctgcaga cggtggcgaa tcaagccgcg ctcgcgctgg
      961 gtacgaccat cctgtgcatt gaccgtgccc ggcgcctcgc tgtcgagacc gagcgcaacc
     1021 gcatcgcccg tgacatccac gacacggtgg cgcaatcgct ttttggcatc gtctatagcc
     1081 tggatgcctg cataacgatg cttccacaaa aagccgacga tgtcaaaaac gaactgatcg
     1141 aattgcgctc attagccaat agcgcgcacg atgaagtccg ccgctcgatc tttgacttgt
     1201 ggccgtccgc actgaccatc gaacttttca tggccgatct gacgaattat gtcggcagtt
     1261 gttgccgtcc acactcgttc aatattattt tcaccaatca gggtgatttc aattcgctct
     1321 cagccggctt gcgcagaacc gtctatcgca tggcgcagga ggctttggca aatgccgcac
     1381 gccactctgg agcccaatcg gcccgtctgt gcctgacggt gactgctgac gaggtttatc
     1441 ttgccgtctc agatgaaggc aatggcttcg atccagccat ggcgctgtca cgcagtcaaa
     1501 accgtgaaca ttttggcttg caaggaattc aggaacgtgc ccgcgccatg ggcggtgatt
     1561 gcgagatcgt ttcccagccc gatgcaggca cacacattat gatcaacctg cccatcaagg
     1621 taccccacca tgcctgaagc cattcgcctg ctgatcgtgg atgaccacgc cgtggtccgc
     1681 aaaggcctgg caatggtcct gcgtctcgaa ccagatatcg aggtcgtcgg cgaagcgggg
     1741 aatggacgaa tcggcctgga tgccgcgaga agtctcaatc ccgatatcgt cctggttgac
     1801 ctggtcatgc cggaaatgga tggccaggag atggcccttg ccttgcggag gtcacatcct
     1861 aaaatcaaga tcatgatgct gaccggcact gaagtggatg accggatctt tgacctcgtg
     1921 gccgtgggca ttgaaggcta tgtgctaaaa aatatcgaac ccggcgaatt agtgcgcgcc
     1981 atccatgctg tcgtacatgg cgaggcatat cttcatcccg acgtgatgaa aaaagttctg
     2041 agtcacatgc acagccaggc acaacaaccc actccatccg tttcattgac tccgcgtgaa
     2101 ttggaagtgc tcgaatggat ggcaacgccg aacacctacc gccagatagc caagcaatta
     2161 accgtgggcg aggaaacgat ccgaagccat gccaagaaca tccttgataa gctgaaacaa
     2221 cccaaccgtg cccaagctgt gctggcagcg atgaagatgg ggctgctgga aatcaaatag
     2281 ggtaagttcg cttcagcaaa tcaagattcc gatattccag aacatcacaa gataaaaagg
     2341 ttggggcaat ttcgtcctgt tctttggcct acccccaaac acggacaggt agtcctccgt
     2401 cggcgctact cacttttggt acagacacaa attcacggtt acagaagccc aaagactttc
     2461 ggaagccgca ccagcttttg aacgttcaga ccgttaggtc gttggttcat gcaaaatccc
     2521 ctttatgagt agactctttg aatcgaagac cgccgggctc acctaaattg gttaacctgg
     2581 cggtcttcaa ttgagactag tagcgggggc aggatttgaa cctgcgacct tcgggttatg
     2641 agcccgacga gctgccactg ctccaccccg catcgaagcc cgccgatgtt accaaagtat
     2701 ccagcaaaag tcaagcgaca tttcctgaca ctgcaagaca ataccgcaaa agccaatagt
     2761 acctctttca tatctcctga cttgataaga tttgcatgtt gtagtttaat agaatcaacc
     2821 ttaaaaattt gaattacatt acagggaaac ttttgttaag atttagggta taatgatttt
     2881 gtcgttgcca gcaacagact gcaattatcg acggatctat tcttcaaatg aaaaagacca
     2941 ctctcatcgt catcgaagga cgacacgcag aaatcccttc tttcgcaaat gatttgcaaa
     3001 agaagggatt tgatattcgt tcgttccaga acggcggaca agccgcctcc aagcttaagc
     3061 agatcaatcc ggctgtggct gtcatcaatg ctgcctcttt acgaagtacc ggtattcgta
     3121 tttgtctgtc attgcgcaag aaggatgcca aactgccgat catcctgatc ctcgaaaaaa
     3181 ccagacccgt ggataaggaa ctcgccgata cagtgctggc tttgcctttt accgtgcaga
     3241 agctggtcaa ccgtatcaag gcgttgatgc ccggtgacgg taataacgtg atcagcgtgg
     3301 gccctattcg actggatgta gaacatcggc gcgtcaaatg cctgggcaag agcaccaagc
     3361 tcacgccgcg cttgatcaca cttctcagcc tcttgatgga taagcacggt gaagtggtcg
     3421 aacgtgaagc gttgttcaaa aaagtttggg aaacgaatta caccggcgat acccgcacgc
     3481 tggatgtgca catctcgtgg ttgcgacgcg caatcgaact tgacccggat aaccccaaat
     3541 tcctaaagac catccgcggt gttggctacc ggctggatgt tta
//
LOCUS       DASTJP010000554         5531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_571261, whole genome shotgun sequence.
ACCESSION   DASTJP010000554 DASTJP010000000
VERSION     DASTJP010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571261"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFQ23_16130"
     CDS             <1..218
                     /locus_tag="VFQ23_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908180.1"
                     /translation="TVDLTLAAEFIVQPTQTRIPTFTPPPPLEVPQFNEAGNSSQPSG
                     TALGILILLLVIIGGVGLALSFVLRTQ"
     gene            221..850
                     /locus_tag="VFQ23_16135"
     CDS             221..850
                     /locus_tag="VFQ23_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9908181.1"
                     /translation="MNVVSKARIFGWIFVIVSVGLLGLFAPGGPVALAQQPTGNVPTV
                     TGTPEGPVVKVDQSIPQIRVYAGPSSFDYPAIGVLLGNETAPAIGRARGREDWIQIRY
                     PGVPGSVGWVFGLYVSLSPGALLPVVELPPTPTPLSTPTINPTLEAAFIGQLTPTRLP
                     TFTPPAPLDRPAFEEPVSATAANVPVGLIILGLGLFGFFGAVISYLRGR"
     gene            complement(901..1305)
                     /locus_tag="VFQ23_16140"
     CDS             complement(901..1305)
                     /locus_tag="VFQ23_16140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9908182.1"
                     /translation="MLQELETKVLTETNIPTITLSMAASDAVKNILSERNLEGYALRV
                     YVAGGGCCSVNFGMALDNNFRDVDTTFDANGVKVVVDEVSIDYLRGATIDFVNDPVRG
                     AGFAVDSPNAKGHEHAHGEEGCGCGGSCSCQN"
     gene            1525..3936
                     /locus_tag="VFQ23_16145"
     CDS             1525..3936
                     /locus_tag="VFQ23_16145"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET9908183.1"
                     /translation="MSVMGLTPLETAAIWAVFGVAIVGLAYAVFLRSQILREDKGTPQ
                     MQEVWNAIKEGADAYLRRQLRSILPLIGILTVALFLSVYIVPPSPEAQERFPELTDAQ
                     LKLWIGIARGIAFIMGAGFSLAVGQIGMRMAIEGNVRVASASKRSFGDALRIAYRAGT
                     ITGMLTDGLGLLGGTIIFIILGIAAPDALLGFGFGGTLLALFMRVGGGIFTKAADVGA
                     DLVGKVEAGIPEDDPRNPAVIADLVGDNVGDCAGMAADIFESYEVTIVSGLILGLALW
                     HITDRLEWIIFPLMVRGIGVLSSIVGTYFVKAGKTGKSVDAMNAIFSGFLTSAVISAA
                     LFFIAGFFYLNTPAMNQWGGWWRLPTAVGVGVLLAIVIDRLTEYFTGTHAAPVNDIKK
                     AADTGPATLILNGVSVGFESSVWSVLVIALTIVASIFIFGTIPGVEGADRATFILYGV
                     AMTGIGMLTLTGNNVAMDSFGPISDNANGIGEMAWHGQTDKATRDAQQIMADLDAVGN
                     TTKAITKGVAIGSAVIAAVSLFGSFLVDVSRAQTSLGIPVEQQIQTIGIRVDIPQVFV
                     GMLIGGALPWLFSSFAIQAVARAASLIVLEVRRQFKLGVLEGKVKPDYRQAVEISTTA
                     AQKELVSLALLGVVTPIVVGLLLQVEALGGFLAGIIVSGQLLAVFLNNSGGAWDNAKK
                     LIEDEPKDPANNLGKGSERHKAGVVGDTVGDPFKDTAGPALNPMIKVVNLVSVIIAPI
                     VVQYSGGASLGVWAVALVLLGILTWAVIRSKAPAPSMTTVPAAAPVSVTERPASKNST
                     GKKKK"
     gene            4041..5330
                     /locus_tag="VFQ23_16150"
     CDS             4041..5330
                     /locus_tag="VFQ23_16150"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET9908184.1"
                     /translation="MDGNSSVKFMKGIYSWGVKGGLGILDQVIFSGTNFLTSVFLARL
                     LSKDEFGQFAVGLAFLTFFTQIYTSFALEPMSVLGPSDYSDRIAAYLMGQVTLMFLIV
                     GPLAILLVLAFGLSNQSTETRSVLVLSSFSLPLFLFPQLMRRIFYVLSKPGMALFGSV
                     LYLLSLLVVYYFARQNFVLNGVNSLLLISLPGLLAGLILLILLRMEKTVSDKINLSKI
                     LIETWSFGRWLVVSGALIGLATQSQIYLTGVLSNTEEAGGVRILQTFIQPIMLTSTAF
                     SALATPAITSDFASGAYGSMRQKVIVFTLVLGLIALFYELLLILFAGRANQILFDGKY
                     ASFADQISVWGLVPILLSFFWGGMMSLQATKNYFALLIISTCWALFSFIPALIFIPTL
                     GAWGATISIVTGFCAAFVSTWILYGSLVWRKYLKIVG"
     gene            5335..>5531
                     /locus_tag="VFQ23_16155"
     CDS             5335..>5531
                     /locus_tag="VFQ23_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908185.1"
                     /translation="MNTMDMQCNICDATSLPLAKALVLKKHEVQYYACVNCGFIQTER
                     PYWLEEAYSSAIARSDIGLIGR"
BASE COUNT         1205 a         1290 c         1407 g         1629 t
ORIGIN      
        1 cgactgttga cctcacgctc gccgcggaat tcatcgtcca gcccactcaa acccgtatcc
       61 cgaccttcac gccgccgccc ccattagaag tccctcaatt taacgaggct gggaacagct
      121 cacaaccttc cggcactgct ctgggaatac tgatccttct cttggtcatt attggcgggg
      181 ttggactggc tttatcgttc gttttaagaa cccagtaagg atgaacgtgg taagcaaagc
      241 gagaatattc ggttggatct ttgtgattgt tagtgttggt ttgttagggc tttttgctcc
      301 gggtggcccc gttgccttgg cgcagcagcc tacaggcaat gtgccgacag taacaggaac
      361 accagaaggc cccgttgtca aagtggatca aagtattcca cagatccgag tgtatgcggg
      421 tccaagttcg tttgattatc cggcaattgg cgtgctgcta gggaatgaaa ctgccccagc
      481 catcggtcga gcgcggggcc gcgaagattg gatccagatt cgatatccag gcgtaccggg
      541 ctccgttggc tgggtatttg ggctctatgt atcgctttcg cctggcgctt tgcttccggt
      601 tgtagaactt cctccgacgc caaccccact ttcaactccg acaataaacc ccacattgga
      661 agctgcgttc attggccagc tcacgccgac tcgcttgcct actttcacac ctcctgctcc
      721 gctcgaccgg cctgcctttg aggagcctgt ttcggctact gcagcaaatg tcccggtggg
      781 gctcataatt cttggattgg ggctctttgg attttttggc gctgtgattt catatcttcg
      841 tggaagataa aaaaaagacc gagtactcgg tcttttttta tgcttcttaa tatcagtaat
      901 ttagttctgg caggagcagg aaccgccgca tccgcatcct tcttcaccgt gggcgtgttc
      961 gtgacccttg gcatttggac tatctacggc aaagcccgcg ccgcgcaccg gatcgttgac
     1021 aaaatcaatg gttgcgccac gcagataatc gatggaaacc tcatcgacca ctacctttac
     1081 accatttgcg tcaaacgtcg tatctacatc tcggaaatta ttatccagag ccatgccgaa
     1141 gttcacacta cagcatcctc caccggctac gtacacacgc aaggcgtatc cttccaggtt
     1201 gcgctcgctc aggatgtttt taactgcatc agaagctgcc atcgacagcg tgattgtcgg
     1261 aatgttggtc tcagtcagta ctttcgtttc aagttcttgt aacatggtat ctccttatgg
     1321 agtgaagttg gtaaatctta tcaaattagg agccgcctgt caaccaacga agtcgcattt
     1381 cacgaaaccg taatatatgt aaaagttaca ttgagccgtt acctctttgt gatgcttgac
     1441 acagatgtaa tgcgcctgta aaatccgtct gcttggctgg gcgaaacgcc cacattttat
     1501 ttttcgtcag gaggatatat ccaaatgtct gtaatgggac ttaccccact ggagaccgct
     1561 gcaatctggg ctgtgtttgg cgttgccata gttggtttgg cctatgcagt gtttctccga
     1621 tcccaaatcc tgcgcgagga caaaggcacc ccgcaaatgc aggaagtatg gaatgcaatt
     1681 aaggagggtg cggatgccta tcttcgcagg cagttgcgct caattttgcc gctgatcgga
     1741 attctcactg ttgctctctt tttatcagtt tacattgttc ctccttctcc cgaagcacaa
     1801 gaacgcttcc cagaattgac tgatgcgcaa ctgaagttat ggatcggcat tgcgcgtggt
     1861 atagcattca ttatgggtgc gggcttctcg ctggcagttg gccagatcgg tatgcgcatg
     1921 gcaatcgaag gaaatgtgcg cgtcgcttca gcttccaagc gctccttcgg tgacgcgctg
     1981 cgtattgcgt atcgggcagg tacgatcaca ggcatgttga cggacggtct tggtcttctt
     2041 ggtggaacca tcatttttat catattgggg attgctgccc cagacgcttt gcttggtttt
     2101 ggttttgggg gaacgcttct cgcgttgttc atgcgtgtgg gtggcggaat tttcacgaag
     2161 gcagcggacg taggcgctga cttggttgga aaggtcgaag caggcattcc tgaggatgat
     2221 ccgagaaatc ccgcggtgat cgccgacctc gtaggtgaca acgtcggtga ctgcgcgggt
     2281 atggctgcgg atatctttga atcttatgaa gtgacgatcg tttcgggact tattcttggt
     2341 ttggcgctct ggcacattac cgaccgcctt gagtggatca tcttcccact aatggttcgt
     2401 ggtattggtg ttttgtcatc cattgttggt acttacttcg ttaaagccgg gaagactggc
     2461 aaaagcgttg atgcaatgaa tgcgatcttt agcgggtttc tcacatctgc tgtgatctca
     2521 gctgcgctct tcttcattgc cggcttcttt tatttgaaca ctccagccat gaatcaatgg
     2581 ggcgggtggt ggcgcttgcc tactgctgtg ggtgtcggcg tcctgcttgc aattgtgatt
     2641 gaccgtctga ctgaatattt caccggaacg catgccgctc ccgtaaatga tattaagaaa
     2701 gctgcagata cgggccctgc aacgctgatt ctcaatggcg tctcggtagg ttttgagtca
     2761 tcagtctggt ctgtgctggt aatcgccctg acgattgtag cctctatctt tattttcggt
     2821 acgatcccgg gtgtagaagg ggcggatcga gcgaccttca ttttgtatgg cgtcgccatg
     2881 accggtatcg gaatgctgac tctgaccggt aataatgtgg ccatggactc gtttggtcca
     2941 atctctgaca atgcaaatgg tatcggagaa atggcctggc atggccagac cgataaagcc
     3001 acaagagatg ctcagcaaat tatggctgac ctggatgcag ttggtaacac tacaaaagcg
     3061 attactaaag gtgtagcaat tggctctgca gtcatcgcgg cggtttccct gtttggatcc
     3121 ttcctggtgg atgtatcccg agctcagaca agccttggta ttccagtaga acaacagatt
     3181 caaaccattg gtattcgcgt ggatatccca caggtctttg ttggcatgtt aattggtggc
     3241 gcactgccgt ggctgttctc atcctttgcc attcaggctg ttgcgcgcgc tgcatctttg
     3301 attgttctgg aagttcgacg tcagttcaag ctaggcgtgt tggaaggcaa ggtcaagcct
     3361 gattacagac aggcggttga gatttctacg acggctgccc aaaaggagtt agtttccctt
     3421 gcgttgctcg gtgtcgtaac cccgattgtg gtaggcttgc ttcttcaagt tgaagccttg
     3481 ggcggtttcc tggcaggcat cattgtctca ggtcaattgc tggctgtgtt cctgaataat
     3541 tccggtggtg cttgggataa tgccaagaaa ctgatcgaag atgaaccgaa ggatccagcg
     3601 aacaatctcg gtaaaggttc cgaacgtcat aaagccggtg tagtaggtga taccgtcggc
     3661 gatccgttca aagatactgc tggccctgca ctgaacccta tgattaaagt agtgaatctt
     3721 gtgtcggtga tcattgcccc cattgtggtg caatattccg gcggcgcttc actgggtgtt
     3781 tgggctgtcg cactggtttt gcttggcata cttacctggg cagtgatccg ttcgaaggct
     3841 ccagctccgt cgatgacaac agtaccggct gctgctccgg tttctgtcac agaaagaccg
     3901 gcttcgaaga actcaacagg caaaaaaaag aagtaagcag agaagatgtt ggaatcaatg
     3961 gctcctgatc tagttcagga gccatttttt gttaattctt agcaatgttg ctttatcttt
     4021 gctgggctat aattttgcat atggatggta acagttcggt caaatttatg aagggcattt
     4081 acagttgggg agtgaaaggt gggctaggca ttcttgatca ggtaattttt tcaggcacaa
     4141 actttctgac atcggttttt ttagcaagac tcttaagcaa ggatgaattc ggacaatttg
     4201 ccgtaggcct tgcttttctt acatttttta cacaaattta tacatcattc gctttggaac
     4261 cgatgagcgt acttggtcca tctgattatt cggatcgcat tgcggcttat ctgatgggtc
     4321 aagtcacgtt gatgtttctg atcgtaggac cgctggcaat cttattggtt ttagcttttg
     4381 ggctgagcaa tcaatcaacc gaaacacgat cggtgctagt tctctcatcg ttcagcttgc
     4441 ctcttttctt gtttccgcaa ctaatgcgac gtattttcta cgttttatca aagccgggta
     4501 tggctctttt tgggtccgtg ttatacctgt tgagcttatt ggtggtttat tactttgcca
     4561 ggcaaaattt tgttctcaat ggtgtaaaca gcctcttgct gatttcactt ccagggctac
     4621 tagctggctt aatattgcta atccttctga gaatggaaaa aactgtgtct gataaaatta
     4681 acttatcaaa aatcctcata gaaacatggt cctttggaag gtggcttgtt gtttcaggtg
     4741 cattaatcgg gctggcgact caatcacaaa tctaccttac gggtgtgctg tcaaatactg
     4801 aagaggctgg tggcgttcga atccttcaga ccttcatcca gccgatcatg ttaacctcta
     4861 ctgcttttag cgcccttgca accccagcaa taacatcaga tttcgcctca ggagcgtatg
     4921 gatccatgcg gcaaaaggtg atcgtattca cccttgtgtt ggggctgatt gcgctgtttt
     4981 atgaattgct tcttatttta tttgctggtc gtgcgaatca aatcctcttc gatggaaaat
     5041 atgcatcctt tgcggaccag atctctgttt ggggacttgt tcccatattg ttgtcgtttt
     5101 tttggggagg aatgatgtcc ctacaagcaa cgaaaaacta ctttgctttg ttaatcattt
     5161 caacctgctg ggcactgttt agctttatcc ctgcccttat cttcattccc actttgggtg
     5221 cctggggtgc cacaatcagc atagtgactg gcttttgtgc ggcatttgtg agcacatgga
     5281 ttttatacgg atccttggta tggcgaaaat atttgaaaat tgttggttga gtgtatgaac
     5341 accatggaca tgcagtgcaa tatctgcgat gcgacaagtt tgccgctggc aaaggctctg
     5401 gttctcaaga aacatgaagt tcaatattac gcctgtgtca attgcggatt cattcaaacc
     5461 gagcggccct attggttgga ggaggcatat tcttccgcca ttgctcgcag cgatattgga
     5521 ttaatcggtc g
//
LOCUS       DASTJP010000555        26307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_466114, whole genome shotgun sequence.
ACCESSION   DASTJP010000555 DASTJP010000000
VERSION     DASTJP010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 26307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26307
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466114"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFQ23_16160"
     CDS             <1..798
                     /locus_tag="VFQ23_16160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9908186.1"
                     /translation="LAAVQSDEYYTDIQRAKNVEAISHVLLLIIHRLNNQISLIPVEA
                     AMALEEIKNSPLSADHSKVVRKHLRKIIQTGRQITNQILEIYRPFTSPLEKVNLFALC
                     SELEKHVNLPSNVNLAIYLDPNISEVLASKTTLFMILENMLQNSISQLPDGGRIWIKS
                     EPTDADWAHVHIYDNGVGIPPDKLRKLSRFLFDFDTKQFNEPRSKKGLGLGLSLAYWF
                     VKRLGGDLQIESRVTEIDHGTRITLKLPRSVERQWNRLGPLMDPHEI"
     gene            788..1498
                     /locus_tag="VFQ23_16165"
     CDS             788..1498
                     /locus_tag="VFQ23_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908187.1"
                     /translation="MKSNTNLKQQKASAEVLIVEDQQDWRNHFFNALSGQGYGVTKAS
                     NVQDARLYLQRIQPDVVILDMHLGNAQGWTLLDETKMPPFGPQIIVVTGMPGIFNDPK
                     QIMRLMSKRDFEGFEVFHFLQKSEFNPQLLRTIVRRALDVAEDSMKGIPALPLTLKDF
                     HEVNSILTKKKFEALTERESEVLKRLILGDSNPEIAIVLSLANETIKTHVKSILTKFQ
                     LDGRGKILPFVIKMGLIK"
     gene            1668..2195
                     /locus_tag="VFQ23_16170"
     CDS             1668..2195
                     /locus_tag="VFQ23_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908188.1"
                     /translation="MRALSNLVNFSLIIITFSLIALGASMVAIPQLLGKIDELIAIND
                     DLENQIAILIAEKEDQNRKLVEAQSILFNQGILIKQLENELNNALAKHRELQVEIRML
                     QIQIDGMTIAQKAAPTLQILSLGILLVANRIKKAPTLSVNARGLQDKNTVTVRMTREQ
                     VKEYSRWRRTEKSKS"
     gene            2332..6312
                     /locus_tag="VFQ23_16175"
     CDS             2332..6312
                     /locus_tag="VFQ23_16175"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9908189.1"
                     /translation="MARRKAGDKKETNKKNRAASVQADSYSIAFDQLHIGRDVSGDIR
                     IGHTIGYTAEQVSVLLKQITTTLEPKKFDGRCPYKGLDVFEEEDAELFFGRERLVNEL
                     VGRVKESRTVFITGPSGSGKSSLVRAGLIHALKNGVIKNSDRWLYETMKPGREPLKDL
                     ALAFSRLKSPELEDYFLAHSAETDILNKCAESVLSGRKDQRFVLFIDQFEEVFTQINS
                     EEERVAFINMLAHAGTVENGRVIVLFAMRSDFVSNCATYPALNELLSQEFRQIGAMQP
                     EELVSAIALPAMHVGLPVENELIAQIINDMRGEPDALPLMQFALKDLFDAKQAEGGVI
                     ALTLKDYLQHGGIHKSLERHADATFAKLSESEQELTRSIFSGLIEIGRGTQDTKRTAL
                     FDELIPANTRAEDVSVIVQKLADARLIITDEQAGKDTVTISHEKLIEAWPWLKKLVNE
                     NRDVIALQNEIAGDAREWEDHKRDSSYLYTGARLANAREKLEANKLVLSGLAYEFVSA
                     GQAQQQRSQRTQILGVAVPVGLVIIAIILFSYLSAKNSANIAKQSEDFANTQQASAST
                     SDALAVIAQANEDEAQKQATIARAGELAAQAINQRETNLPLSSLLGIEAYRTKVTLQT
                     KSLLLENTQYDPELEGFFFNNPTTSFTAFSDDGKILASVHDNNEVIIWDLETHEQISQ
                     IISKIYVLSIAFSPDNSKLIIGGRGGILISDSNTGEDLSQIDTDTDVYSMIFGANDQT
                     LITAECEKNQDDNSTPCTQGTIKVWNIESKIQIVPALYQYNNIANLEIFSDDRKTFVF
                     QSGLENLAIWDTKTYQLIKNVPIDKTVDKTVNSVLAAAISARAGLLATFDMDGTLLLR
                     SLESNQVISNLPIQKKIVTGGGVDYNSFLNFSPDGKYLALGVCDETDTQTCFSFSVFL
                     WKTDTSELIHQYQLPKLSRIAYGLVFNPNGNNFAITLENGDFTIWALESRQPLAKFLF
                     GHDNEIRQIAFSPNAHIIASTSWDGTTILWDPNTGQPMGNPIRDSYSVAFSPDMNTIA
                     VATYGRSVNILDINSHEVIKKLPVEHSSLLFTDLLFSPDGKMLFTNVTSGGNIDSFAA
                     AWDVVTGKFLSDLWKSPYLSPMQISPDKKILAAQIQGDITLWDVKSLQRISSYPGGFG
                     LAFSPDNETVAIAGGTDPTILWNYKSGQSEEIDKKSIFGEHLSVVSGISDVFFSADGE
                     TLLLRSPFTEKDIFFWDIKSRQLFGEISSVHPSPIRTTRFSPNGKTLALGGCKEYRIL
                     QCIQGEIALLNLDPEFWIDVSCQRAGRNFTRAEWAQYFPNEEYRKTCEQWPLEPEIVA
                     TPSTAP"
     gene            6323..6487
                     /locus_tag="VFQ23_16180"
     CDS             6323..6487
                     /locus_tag="VFQ23_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908190.1"
                     /translation="MDYKLAKGTPRVQADKQSIAIGEIHISGTVTGNITMVHTIVQPA
                     PAGARLMCWT"
     gene            6584..7948
                     /locus_tag="VFQ23_16185"
     CDS             6584..7948
                     /locus_tag="VFQ23_16185"
                     /inference="COORDINATES: protein motif:HMM:NF005034.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9908191.1"
                     /translation="MNDKLTKELQTQTQNFKALLTEYCRLESVAAQNRMMKETADWVE
                     KLLKDTGFETQQITVHGAPNYVYGFIRGKSDFTLLLYNHYDVQPEAPIELWNSPPFEA
                     TERDNKLYARGICDNKAELIARICAMRTILAANGELPINIKWIIEGEEEIGSLHFDAL
                     TREHGDLLKADGTLWEGGGFNEKGQAVITLGARGMLYVEYSVELLKQDAHSGNAHALP
                     SAAWRLLKALSSLKDDNGRILIPGFYDDVRQPTESEKQALRDKVDSDLEAKLLAMYGI
                     DSFLHDKSGYEFELAVFEPTANIAGFLSGYTEKGVKTVLPAKAMAKMDFRLVPEQRPD
                     DILEKLQTHLKAQGFDDVQVKKLGSGDPVVTPIEDPFVQRIKQICNEFANQEPEIIPL
                     GGGTLPLLGAMKNNVGILGISISGNPAYYGSGAHAPNEHIRLADIPKAIEFNVFMLNE
                     LGKM"
     gene            7975..10194
                     /locus_tag="VFQ23_16190"
     CDS             7975..10194
                     /locus_tag="VFQ23_16190"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HET9908192.1"
                     /translation="MEDELAKDAPKVQADNQSIAIGEINITGTVTGNITIGHTIVQPA
                     PTGARLRHELGILLKNVQTTWIHGVLEKSVHDAALLELGLELREEAVDNPWRMVIEGP
                     DQTQQTLPRGRKINEVFDEANRLLLILGEPGSGKTTTLLRLARDLIAEVDDAFTRPVP
                     VILNLSTWTNKAQPLDEWLVAELNSKYRLPKKDGQRWLKERRILPLLDGLDEVRAENR
                     AACVEKINQLVTDYGLQGMVVCSRIKDYTALNVRLAFDRAIYIQPLTPEQVDEYLDEA
                     GDKLSSLRSTLQADEALRNMAQSPLILNVMSLAYQNKSAEDLSNPALNTDKSRRKHLF
                     DTYVTRMFKRKAGAQTYEDEQTKQRLSWLARNMQKHNQDIFLIERLQPSWLSTRNLQW
                     TYLLASRVSVALIYCLLVGLWRWLTNELYQMLLVTIPVGIAVGLIISVLDGLRFERLR
                     RKAENNSTSSLWAEAINFFVIWLSITISVSLVSILILWLITIVTDQSDGLARAVVAGT
                     FFGVLAGPIAALIFGLRGSQGLENDIRTVEALSWSWRDAIRGGIVGWVVAAIFLLVVG
                     ASSGMFTDRGLITVMAFLIFALLGVPLGGILNGLRRKIIETKSNPNQGIWLTIRNALF
                     GGLIVGLSFGSGVGLLTGLVNGSAYGLNAFYSVGASFGLVGALRYGGLDIIQHYILRL
                     MLINQNQTPADYIRFLDYAVDRIFLQKVGGGYRFIHRLLLEHFAEMGEVKSVPIARD"
     gene            complement(10309..10998)
                     /locus_tag="VFQ23_16195"
     CDS             complement(10309..10998)
                     /locus_tag="VFQ23_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908193.1"
                     /translation="MNEPTSVPDWLSSGVTALDSFLKSHPDAEQQVREILDKRLVRMD
                     ANTWNSVFMNLDNYWGKECTDVLYNVTNIAQPDLEKRFQEIGQSATVEVINFLRKIIS
                     LYGPEFDAAFLALSQLPHNWKTFYRDVFYDHVNKRTHLRVRFAKYNGEEPFLEGSADS
                     MLELTILMMQTIGFLPSGDLVGTEMAGQFVQEANKLITFLQPPPLEESVNQPESQPDV
                     QGEKPKSKKNE"
     gene            complement(11049..12398)
                     /locus_tag="VFQ23_16200"
     CDS             complement(11049..12398)
                     /locus_tag="VFQ23_16200"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9908194.1"
                     /translation="MADYEQGIFISYAWGKKDDEHEAIVNQLDKSFQTRRIKMIRDKR
                     DLGYKGSISQFMERIGQGNCVIVVISDKYLRSQNCMFELVEIADGKQFHDRIFPIVLA
                     DADIYDPVKRLGYVKHWEAKRAELAAAIKDVDPANLQGIREDMDLYDRIRDKISGLTS
                     ILKDMNTLTPEMHRDEDFTQIYDAIEEKMKAAKSASSSTRTSEVRENVRAQHTENSEQ
                     SRTISVAASASDELGTIHLRKLRDLETHQSSLRTYVEGYLLLSERWDQCGTAKSILLE
                     KGINKIKAKDPLDLRDLGEIRSSLSSASMSKVLSAELLSDDLQINADRFAQEMHSAQQ
                     EVEKLVKIFKTASPTKMEKMELSLHEALFHLDNSISAIKHLIEVCGTNVRTEIGHIEG
                     LINEIVHSQQSQKLPSAAEYSATINRDRTSIGERNRTVQGAGAVGRSSVQQMESNAT"
     gene            12665..13999
                     /locus_tag="VFQ23_16205"
     CDS             12665..13999
                     /locus_tag="VFQ23_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9908195.1"
                     /translation="MGVMLQTFFWDCPRIDNKEGQWWNYIREQIPSLAKAGFTSLWLP
                     PVHKGANLGGPSMGYDPYDYYDLGEFDQKGSIPTWWGTKADLLSLIAEAHSHKLAVIA
                     DVVLNHNNGGDELELNPLVNQERWTLFNPKSGKFPRNWESFHPSYYESWDEGTFGEMP
                     DLSHRNPYVYTELLKLARWLIEEIGFDGFRYDYVKGYGANTIAAFQEYRYMRDDKPFV
                     PYGVAEYWDSTNNTLHWVEEANFSNSNPVDAFDFPLREVLKSLCDQYGFSLNSLITSD
                     TILGQQPQTTVTFVENHDLRDEGRPIVNDKLLAYSYILTHEGFPCVFWKDYFNHNLAL
                     EGTLNGIAALVDVHEKYASGETNILYVDDNLYIMERTGFGDSPGLIYVLNNRGDNWNG
                     NLVTTQWRNTTFGPVAWWSKSDMARPVNQSTDRDGRAQFYAPPRGYAVYAVK"
     gene            14189..14920
                     /locus_tag="VFQ23_16210"
     CDS             14189..14920
                     /locus_tag="VFQ23_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908196.1"
                     /translation="MNRPSLLLSWLGALLFMAGVFTGLGLSGAFAWAESEARVSSPFN
                     ADSDLGMQCPLILSPAEKGVVTAEIVNLTGEAVKPIFRVEISHGRVPRQIQQTFELGS
                     LKSEHMEWTVDSSDVIFERVILVNAQQSRYKSNPSRSGSCGILVFSLFGLTGIQSFGL
                     VLVVSLLLMFAGGVLWLKDRRPLDKFSKEVFQINSVLFVVIVLALLSTLARWWGMALF
                     FDALALLVMGVIITEFVLFSQKHKD"
     gene            15043..16032
                     /locus_tag="VFQ23_16215"
     CDS             15043..16032
                     /locus_tag="VFQ23_16215"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HET9908197.1"
                     /translation="MGTNNKSVLRWPFVVGYLGIITSMLACMAFGIEVGSTSTKAEAG
                     VIQIDNGVVAVVDESGDSVPVAGTSTFELVAPLEATDPWTVAGRTIATNESTQIEDGL
                     QVGDLVRVRGTILDDSTWLAYSIEPAEEAVDPIIILIGTVDSVDPWVVNGITLNVTAD
                     TVITGEITAGMLVRVEILLLEDGTWEVLSISLLGDITEVQECATVIATVVAVNGNQVQ
                     FLGWPSITLGEDVVIEGSEAEGEPGGVLLNPDADVIVVVCPSEEGQIVIVQIIIINIV
                     INPPGEDVGTPGEGEKVLVCHKPDKKKGGNTLSISSSAVPAHLGHGDFLGACP"
     gene            16132..16626
                     /locus_tag="VFQ23_16220"
     CDS             16132..16626
                     /locus_tag="VFQ23_16220"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9908198.1"
                     /translation="MQIHTDIHEQLRALLDTEGATYRVIEHEPEGRTEVIATIRGNRI
                     EQSIKSIVVQVRLNRKENIYCLANVPGDCRIDFKGIKAHLNADSVAFASREKAQELTG
                     CVIGAIPPFSFNDELRVLADPLIEENEEVVFNAGRLDRSIFMKLDDYIRIAKPQLAKI
                     ALRG"
     gene            16669..17172
                     /locus_tag="VFQ23_16225"
     CDS             16669..17172
                     /locus_tag="VFQ23_16225"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HET9908199.1"
                     /translation="MDSYEKNVDKALNAVEKASAVANKAQIGCITLLANLFFAGFCLW
                     GAYAGSLSWKLEQGGERTTGNVVRLEESNSSEGGCCVYSPVIEFVAGGQTYSFESGNA
                     SSPPAYQVGEEVPVLYDPAKPETAQINSFFERWLFPILIIPAMILAALIVNFFMIRAW
                     RRGESIE"
     gene            17169..18083
                     /locus_tag="VFQ23_16230"
     CDS             17169..18083
                     /locus_tag="VFQ23_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908200.1"
                     /translation="MTVQFTLSARPPFKFQSVVDSHGWRQLAPFSYDELSNTLSYVLR
                     LSNGRVIQLQFHDAVDGVTVETEKLDKLEKKEVTNIVTWMFGLDMDFSSFYNASRGEP
                     KLARAKKLSLGRLLRAPTLFEDVVKTILTTNTLWAATRNMSRKLVDEFGLALRGGSSF
                     SRPVDEALNKKSFPTPEAIAASNPETLKEKIRVGYRAPAIHQLAVRVASGQLDLESLK
                     TSGLPTVELRKELMKINGVGPYAAANLLMLLGRHDFIPIDSSALSVVSQEWYEGNPVT
                     AKEVEKHFTKWGEHKGLAFWFWDWKADQ"
     gene            18095..18442
                     /locus_tag="VFQ23_16235"
     CDS             18095..18442
                     /locus_tag="VFQ23_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908201.1"
                     /translation="MFERHTQPLASQSIFTRRLVRFGFFSGGMILFSLSIGILGYHYF
                     ESLSWIDSVLNASMILGGMGPVDPLQTNAGKIFASFYALYSGIVLLASVGVLATPIFH
                     RFLHRFHIEIEDN"
     gene            18445..19218
                     /locus_tag="VFQ23_16240"
     CDS             18445..19218
                     /locus_tag="VFQ23_16240"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9908202.1"
                     /translation="MDLSLTDKVAIITGASRGIGKAIAEALSAEGMKITLAARSESEL
                     EALARSLPNESLVQAIDLREPDSASKVITSTIEKFGSLDLLVNNAGATKRGDFLTLTD
                     EEWTDGFALKFYAAMRLSRAAWTHLKESRGTIINIIGIGGRTGSAEFAIGGAVNAALM
                     NLTKVLSDRGIQEGVRVNAINPGSIATERLQTRIHKYAQTNGISLEEAAKRLPREMKI
                     DRFGEPAEIARAVAFLASAGAAYIQGAILDVDGGATRTL"
     gene            19249..19800
                     /locus_tag="VFQ23_16245"
     CDS             19249..19800
                     /locus_tag="VFQ23_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET9908203.1"
                     /translation="MKCPVCKDQTLGQINLLSGLPANQCSNCNGVFIPSNAYMAWWKT
                     QGRELPPKAGAIEIDHSWEVKELKLCATCGHIMARYKIFPDTDFYIDRCRNCNGIWLD
                     AHEWDVLIDRNLHDNLNEFFTRPWQDHLRAEETKIHLEQIYLEKLGESDYENVKQFRE
                     WLVNHPMRGMLLAFLQAEDPYKI"
     gene            20410..20775
                     /locus_tag="VFQ23_16250"
     CDS             20410..20775
                     /locus_tag="VFQ23_16250"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HET9908204.1"
                     /translation="MTEFTPLHFLDHPIEVLFDMPPVREKTPDCPNGFIWEGKIYRVV
                     EMLSAWSDFKRRGKMARNMRPEHAAVAASRGSLNVGRFSFRVKVDTNQIFDIYYDRAM
                     KNVDDRKGQWFIYRELEEE"
     gene            20775..21497
                     /locus_tag="VFQ23_16255"
     CDS             20775..21497
                     /locus_tag="VFQ23_16255"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9908205.1"
                     /translation="MDQDSLQPVVTPEPRSYSVPWSVLETWIGIILLALINVGLLFIA
                     MRGSGTDLVQSAALIFIQLAFLLPLVLIFVWRHIPVRYLGFGKFDWNTLGIGCGLLIG
                     SYFFIIIHNLILMALGVDTQGEAIFRFFESLESPLWFFFVGAVLAPIVEEIFFRGFVF
                     QGFRQRYGWVSAMVLSSVVFAAAHLDLVAFFPTLILGCLLAYMFQRSNSVWPGAILHF
                     LVNAFGLCTAYAATQIPGLIPT"
     gene            complement(21550..22800)
                     /locus_tag="VFQ23_16260"
     CDS             complement(21550..22800)
                     /locus_tag="VFQ23_16260"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9908206.1"
                     /translation="MNTQKLPHIVIVGAGFGGMEVAKKLANAPVRITLIDRHNYHLFQ
                     PLLYQVAIAGLVPSQIAYPLRTIFRKQKNISFRMGEVTSVDFNSRYVKLEGSIIAYDY
                     LVLAVGGQTNFFGLDSVEEHGFQLKDIESAIGLRNHLLRLFEQASREVDADKRRALLT
                     FVIVGGGPTGVETAGALAELITHVMVKDYPEMSLADARVLLLEATEHLITAYPDELRK
                     ATRDLLQKKNVEIRTNTILTGYNGQQVMLGDGTQIPSHTLIWTAGVRAAEMLDHLGVE
                     QAGSKRARVESTLQLPGHPEVFVIGDASYFENGNGKPLPMLSTVALQQGKLTAENIGR
                     ILKGQSPKPFHYKDPGLLATIGRNAAVARIWGISFSGFIAWVIWVFLHIYRLIGFRNR
                     ILVMINWAWDYVFYENQVRLITKE"
     gene            23000..24625
                     /locus_tag="VFQ23_16265"
     CDS             23000..24625
                     /locus_tag="VFQ23_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908207.1"
                     /translation="MKRVVPYPLLLILAVVLDRVAISSSQISIAQSIRPIIILILLVG
                     LTLFLIQRFVKDWHYTNFIVMMIPIALLIYRSLYGFFKMNFPQQADYLALAILPLLAV
                     TFAAVVSRRVWQWIRNPDQLAYYFNLVFIFLLGFQGLRLGGPIVRVLSTGTQSHAEAI
                     STLTDDVQLQSKTRPDIYVIVLDGYGRQDVLRDIYDYDNSQFIHQLEERGFYVAAENH
                     SNYVQTPYAMASLWNYDYLQPWDSSTDYPRYLLEPIQNNRVFRLLNEIGYTTVSFEGD
                     LVFTEISDSDVYLSNFLPLNKFEGLLLVDGPFEALSNVFGLKFPIPNNETHRSRIQYE
                     LDTLHQIPESIAGPKIVYAHILATHPPFVLDQDGSLVHQHQPYSLWDGSEFKDTQDAY
                     WNGYREQVIFTNKEIIGVVDDILAKSKAPPIILLMGDHGPASLFNWSFSDPGCLWERT
                     SNLYALLLPGHQNDGTVYSTISPVNSFRVIFNTYFGTDFPLLEDKTFLTSWSEPSIMM
                     DVTNKTDVVAGCTMPDLSESTMDHSLDFLQGGF"
     gene            complement(24641..25288)
                     /locus_tag="VFQ23_16270"
     CDS             complement(24641..25288)
                     /locus_tag="VFQ23_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9908208.1"
                     /translation="MTFIIVFFGWIFSLCLHEFSHAIVAYYGGDYTVREKGYLTFNPL
                     KYTHPVFSIVLPLLFLFMGGIGLPGGAVYIERWRIRNRYWLSAMSLAGPFSNLVCAMV
                     LGLILRFSDVTEAGIWPGISFLLVLQVSAVLFNLIPLPPFDGYNAIEPFLHPVIRMQV
                     DRFRDVAIWVVLLAFWFIPFVGSFFWGAVLTFSTILGVNWDLFVAGLQRFRFWEL"
     gene            complement(25333..26229)
                     /locus_tag="VFQ23_16275"
     CDS             complement(25333..26229)
                     /locus_tag="VFQ23_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9908209.1"
                     /translation="MNTRQATKVLLTTITLLTMYACTPASPPVAPSQVQTIASQTPQP
                     TQTTAPLIDTATATPLTCLTETGRVDSGRLESTNPPQEYLIYLPPCYDQKTEEHYPVL
                     YLLHGQTYTQDQWVRLGAVEIIDRLIQSGESLPFIVVFPDDRYWNLPPGPGFGQRLIE
                     LIIPYIDENYRTLDDRDQRAIGGMSRGAGWALRLGLTRWDLFSSIGLHSLAISQKDVS
                     QLDNWLADIPDSSKLRVYMDIGDNDPELTMAGNIEAQFNEYGLLHEWHLYSGGHTEEY
                     WSAHVEEYIQWYAAGWNKTHPE"
BASE COUNT         6905 a         6227 c         6461 g         6714 t
ORIGIN      
        1 cttgcggccg tgcagagcga tgaatactac accgatatcc agcgtgccaa gaatgtcgaa
       61 gccatttccc atgttctgtt actgatcatc catcgtctga ataaccagat cagtctcatc
      121 cctgtagaag ctgcaatggc gctggaggaa ataaaaaata gcccgctaag cgcagaccac
      181 agtaaagtag tcagaaaaca tctcaggaag atcattcaaa ctggacgtca gatcacgaat
      241 cagatcctgg agatttaccg gccatttacc agcccacttg aaaaagtaaa tttgtttgca
      301 ctctgcagcg agttggaaaa acatgtgaac ctgccttcca atgtcaatct ggcaatttat
      361 ctcgacccca acatatcgga agtcctggca tcgaagacaa cgctttttat gatccttgaa
      421 aatatgcttc agaattcgat ctcccagctt cccgacggag gacggatttg gatcaagagc
      481 gagccaaccg atgcagattg ggcacacgta catatttatg ataacggtgt aggaattccg
      541 ccggacaaac tcagaaaact ttccaggttt ttgtttgatt ttgacacaaa gcagttcaat
      601 gaacccagat caaagaaagg cttaggcctg gggctttccc tggcgtactg gttcgtgaag
      661 agactcggcg gcgacctcca gattgagagc cgcgtgacag agattgatca tggcacccgc
      721 atcacactaa agctaccacg ctctgtagaa aggcaatgga accgattggg gccattgatg
      781 gatccacatg aaatctaata ccaacttaaa acaacaaaaa gcctccgcag aagtcctgat
      841 cgtcgaagac cagcaggatt ggcgtaatca ctttttcaat gccttgagcg gtcaagggta
      901 tggggtcacc aaggcgtcca acgtgcagga tgcccgcctc tatcttcagc ggattcaacc
      961 cgatgtagtt attctggata tgcatctcgg aaacgctcag ggttggactt tgcttgatga
     1021 aacaaaaatg ccgccatttg gtcctcaaat tatcgttgtt acgggcatgc ctggcatctt
     1081 caatgatccg aagcaaatca tgcgcttaat gtccaaacga gattttgaag gattcgaagt
     1141 tttccacttc ctgcaaaaat ccgaattcaa tccacaactg ctccgaacaa tcgtacgaag
     1201 agctttggat gtggcagagg attcaatgaa gggtattcct gctttaccgc tcaccctcaa
     1261 agactttcat gaagtcaatt ccatccttac gaaaaagaag tttgaagcgt taaccgagcg
     1321 tgaatctgaa gtattgaaac ggttgatttt gggtgactcg aaccctgaga ttgcaatcgt
     1381 tctttcgttg gcaaatgaaa ccattaaaac tcatgtaaaa agtattctta caaaatttca
     1441 actggacgga cgcggaaaga ttttgccgtt cgtgatcaaa atgggtttaa tcaaataata
     1501 caatgcttac tttgcagtca tactacctat caagccagcc tgaggctggc ttttttattt
     1561 cacccattgg agtgagaagt ttcactctac agagtgggag agtttgagcg gcttcccttt
     1621 atcatgcagg catcgttata agtccaacaa cataaaagga ggctgtcatg cgtgcacttt
     1681 ctaatctagt aaatttctca ctcattatta tcacgttcag cctgatcgct ttgggcgctt
     1741 cgatggttgc cattcctcag ttgttgggaa aaattgacga attgattgcc attaacgatg
     1801 atctggaaaa ccagattgca attctgattg ctgaaaaaga agatcagaat agaaaattag
     1861 ttgaagccca gagtattttg ttcaaccaag gcattcttat taagcaattg gagaatgaac
     1921 tgaacaatgc cctcgcaaaa catagagaat tacaagtcga aatccgaatg cttcaaatcc
     1981 aaattgatgg catgacgatc gctcagaaag ccgcaccaac acttcaaatc ctatccctgg
     2041 gaatactttt ggttgctaac aggatcaaaa aggcaccaac cttatcggtg aatgccagag
     2101 gattacagga caaaaacact gtgaccgtcc gcatgactcg agagcaggtc aaggagtatt
     2161 caaggtggcg aagaaccgag aaaagtaagt cataagaatc aaaaggctgg cgtaggccag
     2221 cctttttctc tgtcccagat aatgttcact gctgcagttc ggaagcattg cagagcaagt
     2281 ttatactata atcacatcta tcgtttagcc ttcgattcaa gggaacattg aatggctcga
     2341 agaaaagctg gtgataaaaa agaaacaaac aaaaagaacc gcgcggcaag cgtgcaggcg
     2401 gatagctaca gcattgcgtt tgatcagctt catattggca gggacgtaag tggggacatt
     2461 cgcatcggtc atacgattgg gtacacggct gagcaagttt cagtcctact caaacaaatc
     2521 acaaccacac ttgaaccgaa aaaatttgac ggacgttgcc cgtataaagg cctcgatgtc
     2581 ttcgaagagg aagacgccga gttgttcttc gggcgcgaga gattagtcaa tgagcttgtc
     2641 gggcgggtga aggaatcaag aacggtattt attaccggac cctcagggag tggaaaatca
     2701 tcgctggtac gcgcggggtt gatccatgcc ttgaaaaatg gagtcatcaa aaacagcgac
     2761 cgctggttgt atgaaaccat gaagccaggg cgtgaacctc tcaaagatct ggcactggca
     2821 ttctcacgct tgaaatcccc cgaactggaa gattatttcc ttgcccattc ggctgaaaca
     2881 gatattttga ataaatgcgc agagtctgtc ttgagtggga gaaaagatca gcgcttcgtt
     2941 ttattcatag atcaattcga agaggtattc acccagatca actcggaaga ggaacgcgtt
     3001 gccttcatca atatgctcgc acatgcgggg accgtagaaa acggacgcgt gatcgtgttg
     3061 ttcgccatgc gctcagactt cgtctcgaac tgtgccacct accccgcgct caacgaactg
     3121 ctcagccagg agttcaggca gatcggcgcc atgcagccgg aggagctggt aagtgccatt
     3181 gccttacctg cgatgcatgt aggcttgccg gttgaaaatg aattgatcgc gcagatcatc
     3241 aatgatatgc gaggcgaacc agatgcattg cctctgatgc aattcgccct aaaggattta
     3301 tttgatgcca agcaggcaga gggtggcgtc attgctttga ccttgaagga ttacctacag
     3361 catggcggca ttcataaatc cctggaacgc catgcagatg cgacctttgc aaaactaagc
     3421 gagagcgaac aggaactgac acgctccatc ttcagcggat tgatcgagat cgggcgcggc
     3481 acgcaggaca caaaacgaac tgcattgttt gatgaactga taccagcaaa tacgagagcc
     3541 gaagatgttt cagtcattgt tcaaaaactg gctgatgcgc gtttgatcat cacggatgaa
     3601 caggcaggca aagatacagt cacgatctca cacgagaagt tgatcgaagc ctggccctgg
     3661 ttaaagaaac tggtgaacga aaatcgcgat gtgattgcat tgcagaatga aattgcaggc
     3721 gatgcgagag agtgggaaga tcataagcgg gattcaagtt atctttatac gggtgcaagg
     3781 ctggcgaatg cgcgtgagaa gctggaagca aacaagttgg tgctaagcgg actggcatat
     3841 gaatttgtga gcgcaggaca agcgcaacag caacgcagtc aaagaacaca gattcttgga
     3901 gttgcagtcc ctgttggctt ggtgataatt gcgattatcc ttttttccta tctatctgcc
     3961 aagaactcag caaacattgc aaaacaaagt gaagacttcg caaacacaca acaagcatct
     4021 gcaagcactt cggatgccct tgcagtgatt gcacaagcaa atgaggatga agcacaaaaa
     4081 caagcgacaa ttgcacgtgc cggagaactc gccgctcagg caatcaatca acgtgaaacg
     4141 aaccttccac tttcatctct tctgggcatt gaagcctata gaacaaaggt cacactacaa
     4201 accaaaagtc tattgctcga aaacactcaa tatgatccag agttggaggg attttttttc
     4261 aataacccga caacttcctt tactgcattt agtgatgatg gaaaaatcct tgcatcagtg
     4321 catgacaaca acgaggtaat aatttgggat ctcgaaacgc atgagcaaat tagccagata
     4381 atttccaaga tttatgtttt atcaattgct tttagtcctg acaatagtaa attgatcatt
     4441 ggtggtcgtg gcggcatact aatctcggac tccaatacag gcgaggacct tagccaaatt
     4501 gatacagata cggatgttta ttcgatgatc tttggggcta atgatcagac attaattacg
     4561 gcagaatgtg aaaaaaatca agatgacaac tcaactccat gtacacaagg aacaatcaaa
     4621 gtatggaaca ttgaatctaa gatccaaatc gtgcctgcac tgtaccaata taacaatata
     4681 gcaaacctag aaattttcag tgacgacaga aaaactttcg tcttccaatc aggattagaa
     4741 aaccttgcga tttgggatac aaaaacttat caactcataa aaaatgttcc tattgataag
     4801 actgttgata agactgttaa ttctgtgcta gccgctgcga tttccgctag agcaggactc
     4861 ctcgcaacat tcgatatgga tggaaccctc ttgttacgaa gtttagaatc aaatcaggtc
     4921 atttcgaact tgcccataca aaaaaagatt gtgactggtg gaggtgttga ctacaactct
     4981 ttcctgaatt ttagtcccga tggaaaatac ctcgcgttag gggtctgcga tgagactgac
     5041 acgcaaacct gcttttcctt ctctgttttt ctttggaaga cagatacatc tgaactgata
     5101 caccaatatc aactcccaaa attatcaagg attgcatatg ggctggtgtt taatccaaat
     5161 ggaaacaatt ttgcaataac acttgaaaac ggagatttta cgatatgggc gctggaatca
     5221 cgccagcctc tggcaaaatt tcttttcgga cacgacaatg aaatcagaca aatcgcgttt
     5281 tcacccaacg cccatatcat tgcctcaacc agttgggatg gaactacgat cttatgggat
     5341 cccaatacag gtcaacccat gggcaacccg ataagagaca gctatagtgt ggctttcagc
     5401 cctgatatga atactattgc cgtagcaact tacggtcgtt cagtgaatat acttgacatt
     5461 aatagccacg aggtcataaa gaaactacca gtagaacact cttcgctact ttttactgat
     5521 cttttattta gcccagatgg aaaaatgctt tttaccaatg tcacctctgg aggcaatatt
     5581 gatagttttg cggctgcatg ggatgtagtt accggaaaat ttctttctga cctatggaag
     5641 agcccttacc taagccctat gcaaataagt cctgataaaa aaattcttgc tgcacaaatc
     5701 caaggagaca tcacactttg ggacgtaaag agcttgcaac gaatttctag ctatcctggt
     5761 ggatttgggt tagctttcag tcctgacaac gaaactgtcg caatagcagg agggacggac
     5821 ccaacgatac tgtggaatta taaatctggt caaagcgaag aaatagataa aaagtcaatt
     5881 tttggtgaac acctatccgt cgttagcggt ataagcgacg ttttctttag cgccgatggt
     5941 gaaacgcttc tactaagatc cccattcaca gagaaagata tatttttttg ggatattaaa
     6001 tcccgccagc tttttggtga aatatctagt gtgcatcctt ccccaatcag aacaacaaga
     6061 ttcagcccaa atggtaaaac cctcgctctg ggaggttgta aagaatatcg tatattgcaa
     6121 tgtatccaag gcgaaatagc attgttgaat ctagatccag agttttggat cgacgtttct
     6181 tgtcaacgag ctggccgcaa tttcacccgc gcagaatggg cacagtattt tccaaatgaa
     6241 gaatatcgca aaacctgcga acaatggcct ttagaaccag aaattgtagc aacgcccagc
     6301 acagctccat aaagttatta acatggatta caagctagca aaaggcacac cgagagttca
     6361 agccgataaa cagagcattg ccatcgggga gatccacatc agtggcactg tcactgggaa
     6421 tatcacaatg gttcatacca tcgtgcagcc tgcccctgca ggtgcgcgac tgatgtgttg
     6481 gacgtagaat ccgctggcaa ggtccggagt ggcggcaagg aagatagtag agaaggcgaa
     6541 agaagaaaca aagtaaacga tattgatatc gaaggatggt acgatgaacg acaagctgac
     6601 aaaagaactc caaactcaaa cacagaactt caaagccctc ctcaccgaat actgccgcct
     6661 ggaaagtgtc gctgcacaaa accgcatgat gaaggaaacc gcagattggg tggaaaaatt
     6721 attgaaagac accggctttg aaactcagca aattacagta catggcgcac cgaattatgt
     6781 gtacgggttc ataaggggca aaagtgattt cacactgttg ctctacaacc actacgatgt
     6841 tcaacctgaa gctccaatcg aattatggaa cagcccgccg ttcgaagcca cggaaaggga
     6901 taacaaatta tacgcacgcg gcatctgtga taataaagcc gaattgatcg cgcgcatctg
     6961 tgccatgcgc accatactgg cagcgaacgg agaactgcca atcaatatca agtggatcat
     7021 cgaaggcgaa gaggaaattg gcagcctgca cttcgatgca ctgacacgtg aacatggcga
     7081 tctgctaaaa gcggatggca cgttatggga aggcggcgga ttcaatgaga agggtcaagc
     7141 tgtaataaca ctcggcgctc ggggaatgct gtatgtggaa tacagtgtgg agctcctgaa
     7201 acaggatgcg cattccggca atgcgcatgc cctgcccagt gcggcttggc gtttgttgaa
     7261 agccttgtcg agcctcaaag atgataatgg cagaatcttg attccgggat tttatgatga
     7321 cgtccgccaa ccgaccgaat cagaaaagca ggctttgcga gacaaagttg actccgattt
     7381 ggaagccaag ctgcttgcca tgtatgggat tgactcgttc ctacatgaca aaagtggcta
     7441 tgaattcgag ttggcagtat ttgagccgac agctaatatt gctggcttct tgtctgggta
     7501 cacagaaaaa ggtgtgaaga ccgtcctgcc agcgaaggca atggcaaaga tggattttcg
     7561 tctcgtgcct gagcaacgcc ccgatgatat tctggaaaag ctacagactc atctcaaggc
     7621 acagggcttt gacgatgtgc aagtcaaaaa gctggggagc ggcgatccgg ttgtcacgcc
     7681 gattgaagat ccattcgtgc agcgcatcaa gcaaatctgc aacgagttcg cgaatcagga
     7741 accagaaatc atcccgctcg gaggcggaac actccctctg ctcggcgcga tgaagaacaa
     7801 tgtgggaatt ttggggatat ccatttcggg caacccggcc tattatggga gcggcgctca
     7861 cgcccccaat gaacacatcc gattggctga tattcccaag gcgattgagt tcaatgtgtt
     7921 tatgctcaat gagttgggaa agatgtaaac tccggcactt tcacaatagc caccatggaa
     7981 gacgagttag caaaagacgc acccaaagtc caagccgata accagagcat agccatcggg
     8041 gagatcaaca tcaccggcac tgtaaccgga aatatcacca tcggtcatac catcgtgcag
     8101 cctgccccta cgggcgcgcg cctgcgtcat gagctgggca tcctgctcaa gaacgtccaa
     8161 accacgtgga tccatggcgt gcttgaaaaa tcagtccatg atgcggctct gcttgagttg
     8221 ggtttggaat tgcgcgaaga agccgtggat aacccctggc ggatggtgat cgaaggtccc
     8281 gatcagactc aacagacact gccgcgaggc agaaagatca acgaggtctt cgacgaagcc
     8341 aatcgcctgc tgttgatcct gggcgagccg ggttccggca agacgacgac cctgctccga
     8401 ttagcaagag atctaattgc ggaagtagat gatgctttca cgcgacctgt ccctgtcatc
     8461 ctgaatctct ccacgtggac gaacaaagca caacccttag atgaatggct ggttgctgaa
     8521 ctcaacagca aatatcgcct gccgaaaaag gacggtcagc gctggctaaa agagcgccgc
     8581 atcttgcccc tgctggatgg actggatgaa gtgagagccg agaaccgcgc cgcttgtgtg
     8641 gagaaaatca atcagttggt aacagattat ggattgcaag ggatggtcgt ctgcagtcgt
     8701 atcaaggact acaccgcgct caacgtccgt ttggcatttg atcgtgcaat ttacatccag
     8761 ccgctcacgc ctgaacaggt tgatgaatat ttagatgaag cgggcgataa actctccagc
     8821 ttacgttcaa ctttgcaggc tgacgaagct ctgagaaaca tggctcaatc accattgata
     8881 ttgaatgtga tgagcctggc ataccagaat aaatcagctg aggatctaag caatccggct
     8941 ttgaacacgg acaaatctcg ccgcaagcac ttgtttgata catatgttac tcgaatgttt
     9001 aagcgtaaag ccggtgcgca gacttatgag gatgagcaaa caaaacagcg tctttcctgg
     9061 ctggctcgga acatgcaaaa gcataatcag gatatattcc tgattgagcg cttacaacca
     9121 agctggttgt ccactcgaaa tttgcagtgg acttacctcc tcgcatccag agtaagtgtg
     9181 gcattgattt attgtctgct ggttgggttg tggagatggc tgacaaacga gttataccaa
     9241 atgctgctag ttacgattcc ggtcggaata gccgtgggat taataattag tgttctcgat
     9301 ggactgcggt ttgaaagact tcgtcgaaaa gcggaaaata acagcacgtc atccctgtgg
     9361 gcagaagcga tcaacttttt cgttatttgg ttgagtatca ccatcagtgt ttcgcttgtc
     9421 agcatattga tcttgtggct catcacaatc gtaacggatc aaagtgacgg actcgcccgg
     9481 gccgtggtag ccggcacatt tttcggagtg cttgcaggtc cgattgctgc tctgattttt
     9541 ggattgagag gtagtcaagg tttggagaat gacatccgga ccgttgaagc gttgtcctgg
     9601 tcgtggcgcg atgcgatccg cggtggtatc gttggttggg tcgttgcagc catttttcta
     9661 cttgtggttg gtgcaagttc tgggatgttt acagaccgcg ggctaataac tgttatggct
     9721 tttctaatat ttgcattgct tggcgtgccc ctgggcggga ttctgaatgg cttgcgacga
     9781 aaaataatag agacaaaatc caaccccaac caggggatct ggctaaccat aagaaatgca
     9841 ttattcggtg gtttgattgt aggattaagt tttgggtcag gggtcggatt gttaaccggt
     9901 ctggttaacg gatcagctta tggactaaac gcgttttact cagtgggtgc tagctttggc
     9961 ttagtgggcg cgttacgata tggtggtttg gatatcatcc agcactatat cttgcgtttg
    10021 atgctaatca atcaaaatca aactccggca gattacatcc gcttcctcga ttacgctgtt
    10081 gatcgcatct ttctccagaa agtcggtggg ggttatcgct tcatccaccg gctgctgctg
    10141 gagcatttcg cagagatggg tgaggtgaaa agcgttccta tcgccaggga ctaaagcccc
    10201 tggctaccat caggaagcct gcaaagcagg cttaagccgc ctgtggcggc ttcttctaaa
    10261 tagcatggac attaatgtcc gtgcggtgag gttgctgaaa catctctctt attcgttctt
    10321 tttgctcttt ggcttttcac cttgtacatc cggttggctt tcgggctggt tcacagattc
    10381 ttcgagaggg ggtggttgaa ggaaagtgat cagtttgttt gcttcctgaa cgaactgacc
    10441 agccatctct gttccaacaa gatcaccact tggcaaaaaa ccgatcgtct gcatcatgag
    10501 gattgtcaat tcgagcatgg aatcagcact tccttcaagg aagggttctt ccccattgta
    10561 tttcgcaaag cggacgcgca gatgagtacg cttgttcaca tgatcataaa agacgtcccg
    10621 ataaaatgtc ttccagttgt gtggcagctg actcaatgct aaaaaggctg catcaaactc
    10681 tgggccgtac aatgagataa ttttgcgcag aaaattgatt acctcaacag tcgcagattg
    10741 tccaatctct tgaaatcgtt tttccagatc aggctgtgca atatttgtga cattatataa
    10801 aacatccgtg cactctttgc cccagtaatt atcaagattc ataaacacac tattccatgt
    10861 atttgcatcc atccttacca ggcgtttatc gagaatttca cgcacctgct gttcggcatc
    10921 cgggtgcgat ttgagaaatg agtccagggc cgttacacct gacgacaacc aatctgggac
    10981 ggatgtaggt tcgttcataa tttcattctc ctttgcaata tccaatttta gttacctgga
    11041 gtggagctct atgttgcgtt actttccatt tgctgaacgc tggaacgtcc aactgcaccg
    11101 gcaccctgta cggttcgatt acgctcgcca atggaggttc gatcacgatt gatcgtagca
    11161 gaatattctg ccgcactggg caacttctga gactgttggg agtgcacgat ctcattaatc
    11221 aacccttcaa tatgaccaat ttccgttctg acgtttgtgc cgcatacttc aatgaggtgt
    11281 ttgattgcag atatggaatt atcaagatga aacaatgcct catgcaggct aagctccatt
    11341 ttctccattt ttgtaggcga tgccgttttg aatattttga cgagtttttc gacctcttgc
    11401 tgtgcgctat gcatttcctg ggcgaaccgg tctgcattaa tttggagatc gtcagacagc
    11461 aactcagcag aaagtacctt gctcatggaa gcactggaga gtgaagatcg aatttctccc
    11521 agatctctca agtccaacgg atcctttgcc ttgatcttat tgatcccctt ctccaacaag
    11581 atggatttgg cagtgccaca ttggtcccat ctctcactca acagcaaata cccttccaca
    11641 taggttcgta gcgaactttg atgagtttct aaatctctta gttttctgag gtggatcgtt
    11701 cccagttcat cactggcaga ggcagccaca ctgatggtac gcgattgttc tgaattttct
    11761 gtgtgttgcg cgcgcacatt ttctctcact tcactggttc gagtggacga agaagcggat
    11821 ttcgccgctt tcatcttttc ttcaatcgca tcgtagattt gagtgaaatc ctcatcccgg
    11881 tgcatctcgg gcgtgagtgt attcatgtcc tttagaatac tggtcaatcc tgagattttg
    11941 tcccggatgc gatcatacaa atccatatcc tcacgaatgc cttgcaaatt cgccggatct
    12001 acatccttta ttgccgcagc caattccgcc ctttttgctt cccaatgttt tacgtaccca
    12061 agccttttca ccggatcata aatgtcggca tccgccagaa caatcgggaa gatacggtca
    12121 tggaattgtt tgccatcggc aatttccacg agttcgaaca tgcagttttg agatcgcaga
    12181 tatttgtcgc tgatgaccac gatcacgcaa ttgccctgac caatgcgttc catgaattgg
    12241 ctgatcgagc ctttgtaccc caaatctcgt ttatcgcgta tcatcttgat tcgacgcgtt
    12301 tgaaacgatt tgtcaagttg gttgacaatt gcttcatgtt catcgtcctt cttgccccac
    12361 gcatacgaaa taaagatgcc ttgctcgtaa tcggccattc aacaactcct tgtgtcaacc
    12421 gccatttgcc gccacctggg tcgattatat gtttcataga tagctattgt caataaaaaa
    12481 tccttccgag agtaactagc cttatctcgc ggtacaatcc caccaattcc atgtcattgc
    12541 gagccgacga tagtgccttt ctggcggcgt ggcaatctca ttcggtacga tcaatacata
    12601 acaggagatt gcttcgtcgg gcttcgccct cctcgcaatg acatattgaa ttcaggagca
    12661 aacaatgggt gtcatgctac aaacgttttt ctgggattgt ccgcgcattg ataacaagga
    12721 aggacaatgg tggaactata tccgtgagca aattccttca ttagctaagg ccgggttcac
    12781 gtcgctgtgg cttccgccgg tgcacaaagg cgcgaatttg ggcggcccgt ctatgggcta
    12841 tgacccgtat gattactatg acctgggcga gttcgatcaa aagggaagca ttcccacctg
    12901 gtggggcacc aaggcggacc tgctaagtct catcgcagag gcacacagtc acaagttggc
    12961 ggtgatcgca gatgtcgtcc tcaaccacaa caacggcggg gatgaactgg aactcaaccc
    13021 tcttgtcaat caggaacgct ggacgctctt caacccgaag agtggaaagt tcccacgcaa
    13081 ctgggaaagc tttcacccca gctattatga atcctgggac gaaggcacct tcggcgagat
    13141 gcccgatctt tcgcatcgca atccctatgt atatactgag ttgctcaaac tggcgcgctg
    13201 gctgatcgaa gagatcggct tcgacggctt ccgttatgac tatgtcaaag gctatggggc
    13261 gaacactatc gcagcctttc aggaatatcg ctatatgcgc gacgacaagc cgttcgtccc
    13321 gtatggcgtt gcggaatatt gggactctac caacaacacg ttgcactggg tggaggaagc
    13381 aaatttctcc aatagtaatc cggtggatgc gttcgatttc ccgctgcgcg aggtgctcaa
    13441 gtctctgtgc gatcaatacg gttttagctt gaatagtctc atcacttcgg acactattct
    13501 aggacagcag ccgcagacta cggtcacgtt tgtggagaat cacgatctgc gcgatgaggg
    13561 acgcccaatc gtcaacgaca aattgctggc ctatagttat atccttacgc acgagggttt
    13621 tccctgcgtg ttctggaagg attacttcaa tcacaacctc gcattggaag gcacactgaa
    13681 cggaatcgcc gcgctggtgg atgttcacga aaaatatgcg agtggcgaga cgaatattct
    13741 gtacgtggat gataatctct acatcatgga acgcacagga tttggcgata gcccgggctt
    13801 gatctacgtg ctcaacaacc gcggtgacaa ctggaacggc aatctggtca caacccaatg
    13861 gcgcaacacc acgttcggac ctgtcgcctg gtggagcaaa tcagacatgg ctcgccccgt
    13921 caaccaatcc accgaccgcg acgggcgcgc ccaattctac gccccacccc gcggctatgc
    13981 agtatatgca gtgaaataac ataccgctgg tcgagtagcc ccgaacgcta gtgagggacg
    14041 tacacttgca ccgcattgcg ctcggtgcag tgcaggtgtc gagaccacca atttccaaag
    14101 gtaatctttc caagatagat ctgtcttctt acgaatggtc tcggtatgcc cgacgagcac
    14161 ggggcactcg accaacagat tactctttat gaaccgccct tcacttctcc tctcctggct
    14221 tggagctttg ctattcatgg caggcgtatt caccgggctt ggtctctctg gagcgttcgc
    14281 ctgggcagaa agtgaagcgc gcgtttcttc tccgttcaac gccgatagtg acctggggat
    14341 gcaatgccca ttgattcttt cccctgcaga gaaaggcgtg gtcacggctg aaatcgtcaa
    14401 tctgaccggt gaggcggtca aaccaatttt cagagtcgag atcagccacg gccgagtacc
    14461 gcgccagatc cagcagacgt ttgaattagg gtcgttgaaa tcagagcata tggaatggac
    14521 agttgattca tcggatgtca tcttcgagcg ggtcattctg gtgaacgccc agcagtcccg
    14581 ttataaaagc aatccatccc gttcaggttc gtgtggcatt ctcgtcttca gtctgtttgg
    14641 tctgacgggc atccagtcct ttggactggt actcgtagtc agcttgctcc tcatgttcgc
    14701 cggcggagtg ctttggctga aggatcgtcg tccattggat aagttctcta aggaggtctt
    14761 tcaaatcaat tcggttttgt ttgttgtaat tgtcctcgct ttattgagca cactggcccg
    14821 ctggtgggga atggctcttt tcttcgatgc gctcgccttg ctggtgatgg gggtcatcat
    14881 tacggaattc gttctctttt cgcagaaaca taaagactaa gtggttactt tcagaaccgt
    14941 gaagggctgc gtcccccaaa tgtcatgatt gtattgggac tcatacgtgc tatcatgctt
    15001 aagacgtatt tcccatattc atgaaatttt ccggaggaag ctatgggtac aaacaataaa
    15061 tcggtccttc gttggccatt cgttgtgggg tacctgggga tcatcaccag catgctggcc
    15121 tgtatggcat ttggcattga ggttgggtca acctcaacaa aagcagaggc gggagttata
    15181 cagatcgata atggcgtcgt tgcggtcgtg gacgaaagtg gggacagcgt gcctgtcgca
    15241 ggaacttcga ccttcgaact ggtcgccccg ctcgaagcga cggatccgtg gacggtcgct
    15301 ggaaggacca ttgcgacaaa tgaatcaaca cagatcgaag atgggctgca ggtcggggac
    15361 ctggtgcgcg tgcgcggcac tatcctcgac gatagcacct ggctggcgta ctcgatcgaa
    15421 cctgccgaag aggcggttga ccccatcatc atcctgatcg gcacggtaga ctccgtcgat
    15481 ccctgggtgg tcaacggaat taccctcaac gttaccgccg acacggtcat taccggagag
    15541 attactgcag gaatgcttgt ccgcgtggag attctccttc tggaggatgg cacgtgggag
    15601 gtattgagca ttagcctgct gggcgacatt accgaagtgc aggaatgtgc aacggtgatc
    15661 gcaacggtcg ttgcagtaaa cgggaaccag gttcaattcc tgggctggcc atccatcacg
    15721 ctcggcgagg atgtggtgat cgaaggtagc gaagccgaag gcgaaccagg cggagtatta
    15781 ctaaaccctg acgcagatgt gattgttgtt gtctgccctt cagaagaagg acagatcgtg
    15841 atagtacaga tcattatcat caacatcgta atcaatccgc caggtgaaga tgttggaact
    15901 ccaggcgagg gagaaaaagt attggtgtgt cacaagcctg ataagaaaaa gggcggaaac
    15961 acactgagca tatcctcatc tgcagttcct gcgcacctcg ggcatggaga ttttctgggt
    16021 gcctgtcctt gagtagaaag ttgatcgaaa agacaagcgt cgggatgaag tatttcatcc
    16081 cgacgctgtt ttgtattgat aaatctttcc ctgtataatg actcgaaaca tatgcaaatc
    16141 catactgaca ttcacgaaca actccgtgca ttattggata cagaaggtgc cacttaccgt
    16201 gtcattgaac acgagccgga ggggcgcact gaagtgatcg ccacgattcg cggcaatcga
    16261 atcgaacaat ctattaagtc gattgtggtg caggtgaggc tcaatcgaaa agagaatata
    16321 tactgccttg ctaatgtgcc gggcgattgc cgcattgatt tcaaaggcat caaagctcat
    16381 ctcaatgcgg atagcgtcgc ctttgcgtcg cgggaaaaag cgcaggagtt gacaggctgc
    16441 gtgatcgggg cgatcccacc tttttcgttc aacgatgagc tgcgggtgct ggctgatccg
    16501 ctcattgagg aaaatgaaga agtggtattc aacgcgggtc gcctggaccg atccatcttt
    16561 atgaaactcg acgactacat tcgaattgca aaacctcaac tggcgaagat tgctctgcga
    16621 ggctaggctt tacgtatgca agaacatccc atcctgtaga ggtgcatctt ggatagttat
    16681 gaaaagaacg ttgacaaagc gttgaatgca gttgaaaaag cgagtgcagt tgcgaacaaa
    16741 gcgcaaattg gctgtattac tttattggcg aatctattct ttgcagggtt ttgcttatgg
    16801 ggcgcgtatg cgggctccct gagttggaaa ctggaacagg gtggggagag gaccaccggc
    16861 aacgtggttc ggttggaaga gagcaatagc agtgaaggtg ggtgttgtgt atattcgccc
    16921 gtgattgagt ttgttgctgg cggtcaaacc tattcatttg aaagcggcaa tgcctccagc
    16981 ccgcctgcgt atcaggtcgg agaagaagtg ccagttcttt atgatccggc gaaacccgag
    17041 acggcccaaa tcaacagttt ctttgaacgc tggttattcc cgatccttat catcccggcg
    17101 atgatccttg ccgcgctgat cgtcaacttc ttcatgatca gagcctggcg acgaggtgaa
    17161 tccatcgaat gacagtgcaa tttacacttt ctgctcgtcc accgtttaag ttccaatcag
    17221 tcgtcgactc acacggatgg cggcagctgg cgccgttttc gtacgatgaa ttaagcaaca
    17281 ctctttctta tgttctgcgc ctctcgaatg gacgcgtgat ccaactccag tttcatgatg
    17341 ccgtagatgg cgttaccgta gaaacagaaa agctggacaa acttgagaag aaagaagtaa
    17401 caaatatcgt cacctggatg ttcggtctcg atatggattt ttcatccttt tacaacgcct
    17461 cgcgcgggga acccaaactg gcgcgtgcaa aaaagctctc gctgggcagg ctccttcgcg
    17521 caccgaccct ttttgaggat gtagtcaaga cgattcttac gacgaacaca ctctgggctg
    17581 cgactcgaaa tatgtccagg aagttggtgg atgaattcgg actcgcgctg agaggagggt
    17641 caagtttttc ccgacccgta gacgaagcgc tgaacaaaaa atccttcccc acacctgaag
    17701 cgatcgcggc ctccaacccc gagactctca aagagaaaat acgcgtgggc taccgcgcgc
    17761 cagccattca tcaacttgct gttcgcgtgg cgtcaggtca gctcgatttg gagtcactga
    17821 aaacgtcagg tttaccgact gtcgagttgc gcaaggagtt aatgaagatc aatggcgtgg
    17881 gtccctacgc ggccgcgaat ttgctaatgc tcctcggacg tcacgatttc attcccattg
    17941 attcctccgc gctcagtgtc gtttcgcagg aatggtatga gggcaatcct gtcactgcaa
    18001 aagaagtgga aaaacacttt acaaaatggg gtgagcataa gggtcttgcg ttctggttct
    18061 gggattggaa agccgatcaa taacggaggt acaagtgttc gagcgtcata cacagccatt
    18121 agccagccaa tccatattta cgcgccgcct ggtgcgattt ggattctttt cgggcgggat
    18181 gatcttgttt tcgctatcga taggaattct ggggtaccac tattttgaat cgctttcatg
    18241 gattgattct gtgctcaacg cctccatgat cctgggaggc atgggcccgg tggatcctct
    18301 tcagaccaat gccgggaaaa tatttgcgtc attctatgca ttgtattccg ggattgtcct
    18361 gctggcctcg gtgggtgtcc ttgccacacc gatctttcat cgcttcctgc atcgttttca
    18421 catcgaaatt gaagataact gatcatggat ttatcactca ccgataaagt cgcgatcatc
    18481 accggtgcca gtcgcggtat cgggaaggct attgcagagg cgttatccgc cgagggaatg
    18541 aagatcacct tggctgcacg ctcagagtcg gaacttgaag cgctggcaag gtctttgccg
    18601 aacgagtctc tcgttcaggc gattgatcta cgcgagccag attctgcaag caaggtgatc
    18661 acctcgacca ttgaaaaatt tggcagcctc gatctactgg tcaacaacgc aggtgccacc
    18721 aaacgcggtg actttttgac tctcactgat gaagaatgga cggatggctt cgccctgaaa
    18781 ttctatgcgg cgatgcgcct ttcacgcgcc gcctggactc acttgaagga aagccgcggc
    18841 accattatca acatcattgg cattggcggg cgtacaggca gcgctgaatt cgccatcggc
    18901 ggcgcggtca atgcggcgtt gatgaaccta accaaggtcc tttcagaccg cggcatccaa
    18961 gaaggtgtgc gcgtgaatgc catcaacccc ggctccattg cgactgaacg cttgcaaact
    19021 cgtattcata agtatgcaca aacaaatggc atcagcctgg aggaagccgc aaaacgtttg
    19081 ccgcgggaaa tgaagatcga tcgttttggc gaacctgcag agatcgctcg cgcagtcgcc
    19141 tttctggctt ccgcgggagc cgcatatatt cagggcgcaa tcctggatgt agatggcggc
    19201 gcaacacgga cgttataatt cgacaagccc atcattaccg gaggcaatat gaagtgccca
    19261 gtctgcaaag atcaaacgct cggacagatc aatctcctca gtggattgcc tgccaatcaa
    19321 tgctcgaatt gtaacggtgt ctttattcca tcgaacgcgt atatggcctg gtggaaaact
    19381 caggggagag aactcccccc gaaggcggga gccattgaaa tcgatcactc gtgggaggta
    19441 aaggaattaa aactttgtgc cacttgtggt catatcatgg cacgctataa gatcttcccc
    19501 gacacagact tctatattga ccgatgccgc aactgcaatg ggatctggct cgacgcacat
    19561 gaatgggatg tgttaatcga ccgaaacctg cacgacaatt tgaacgagtt ctttacgcgc
    19621 ccctggcagg atcatctccg cgcagaagag accaaaattc atttagagca aatctatttg
    19681 gaaaaacttg gcgagtccga ctatgaaaac gtcaagcagt tccgcgagtg gctggtgaat
    19741 catcccatgc gaggcatgct gctggcattt ttgcaggcag aagatccgta caaaatataa
    19801 ggatatagag catgaatgtt gtagcgggtc tcgtttccgt ggctataggg attttagtaa
    19861 ccatcctaac gcgcaggtgg ttttttggct ctgatgagaa tttttggaaa agctttggta
    19921 atgattttga ctcagggttt gttcttggac gacctaagag atatatgaag tatttgcatg
    19981 acaatggtgg acagtcaaca gtagtcgcct ggttgacaac aggaacattg agcactctcc
    20041 tctcatatgc aatgcttagt ctactatttt attgaaatat gtgatgcaca atgaacgcat
    20101 ccgtaactgg gtgatatttt caatcgtttt ctcatatgtc atagtatcca gggatcggct
    20161 catctttgca gtattttcga tttgggttgg cgcgtgggct atggcaaaga tgtataaata
    20221 tctctttaac tctgtgaagg attctgcaaa tctcacattg gaaagattca ttccagataa
    20281 attcatctgg cttggtttgt tatctgaatc taccagaaaa gaaacttttc ttatcattgt
    20341 atggtggttg gttggaattt tggcagcatt aatgacatac aatttgctca agataatatt
    20401 tatcacgtca tgacagaatt tactcctctt cactttctcg accatcctat agaagtcctc
    20461 ttcgatatgc ctcctgtgcg cgaaaagacg cccgattgcc cgaacggatt tatctgggag
    20521 ggtaaaatct atcgcgttgt agaaatgctt tcggcatgga gtgatttcaa acgccgcggc
    20581 aagatggcac gtaatatgcg ccccgagcac gcggcagtgg cagcgagccg aggttcgtta
    20641 aatgtaggca ggtttagttt cagggtaaaa gtggatacaa atcaaatctt cgatatttat
    20701 tatgaccgcg ccatgaaaaa tgtggatgac cgcaagggtc aatggttcat ttatcgcgag
    20761 ttagaagagg aataatggat caagactccc tgcagcctgt cgtcacgcct gaacctcgct
    20821 catattctgt cccatggagc gtcctggaga cctggatcgg catcattctg ttggcattga
    20881 taaatgtggg attactcttc atcgccatga gaggttcagg gacagatctc gtccaaagcg
    20941 cggcgctgat ctttattcaa ttagcgtttc tcctgccgtt ggtgttgatc tttgtatgga
    21001 ggcacatccc cgtacgatat ttgggctttg gaaagttcga ctggaatacg ttgggcatag
    21061 gctgcggact cttaatcggc agctacttct ttatcatcat acacaacctt atcttgatgg
    21121 cgctcggcgt agatacacag ggcgaggcga tcttcaggtt ctttgagtcg ctcgaatctc
    21181 cactttggtt tttctttgtt ggcgctgtcc tggcgccgat tgtcgaagag atattcttcc
    21241 gagggtttgt atttcaaggc tttcggcagc gatatggctg ggtcagcgca atggtgttga
    21301 gctccgttgt cttcgcggcg gcacatttgg atctggttgc cttcttccca accttaattc
    21361 tgggctgttt gctcgcctac atgtttcaga ggtcaaattc ggtttggccc ggcgccatcc
    21421 tgcattttct tgtcaatgcc tttgggttgt gcacagcata cgccgccact caaattccag
    21481 gcttgatccc cacctgaaaa gattatatat tcgcccagca gttaagctgc tgggcgaacg
    21541 gaaaacgatt tactccttcg tgatcaaccg cacctgattt tcatagaaga cgtaatccca
    21601 cgcccagtta atcataacca atatacgatt gcggaaaccg atcaacctgt agatgtgcag
    21661 gaagacccat atcacccagg caatgaatcc gctaaacgag attccccaga tacgcgccac
    21721 tgccgcgttg cgcccaatgg tggccagcag gccagggtcc ttgtaatgaa acggtttggg
    21781 actctgtccc ttcagaattc gcccgatatt ttccgccgtg agcttaccct gctgcaaggc
    21841 gaccgttgaa agcatgggca gtggcttccc atttccgttc tcgaaataag acgcgtcgcc
    21901 gatgacgaat acttcaggat gtcccggcag ttgaagggtg gactcgactc gggctcgctt
    21961 tgagcctgct tgctcaaccc caaggtgatc caacatctcg gctgctctca cgcctgccgt
    22021 ccaaatcagc gtgtgcgaag ggatttgcgt cccatcccca agcatgacct gctgcccgtt
    22081 atatcctgtg agtattgtgt tcgtgcggat ttcaacgttt tttttctgca agagatctcg
    22141 cgtcgctttg cgcaattcat ccgggtaggc cgtgatcaaa tgctctgtgg cttccagtaa
    22201 taggacgcgc gcgtctgcca ggctcatttc agggtaatct ttaaccatca catgcgtgat
    22261 cagttcagcc agcgccccgg ccgtctcaac ccctgtgggc ccaccgccaa cgatgacaaa
    22321 cgtcaataaa gcccgccgct tgtccgcatc cacttctcgg ctggcctgct cgaatagcct
    22381 gagcaagtgg tttcgcaagc caatcgcgct ttcgatatcc ttaagttgaa aaccgtgctc
    22441 ttcaacagaa tccaaaccaa aaaagttagt ctgcccgcca accgcaagaa ccagataatc
    22501 ataggcgatg atcgagcctt ccaatttgac gtagcgggaa ttgaaatcga cagaggtcac
    22561 ttctcccatg cggaaagaga tgttcttttg tttacgaaaa atggtacgta gagggtaagc
    22621 aatttgcgag gggaccaatc ctgcaatcgc gacctgatag agcaatggct ggaacaggtg
    22681 atagttgtgc cggtcgatca gcgtgattcg gacaggtgcg tttgccagct ttttggctac
    22741 ttccatgcca ccaaatcccg ctccgacgat gacgatgtga ggaagctttt gagtattcat
    22801 gtgttcctgt ctttcctggc ttcgtttgtg aaatattaca caattattat aactaagacg
    22861 gtcataattg tcaaattgtt cctgcgcatg atgttgctat gaaattattt gatcatttcg
    22921 taactttcgc atcagtccag cgtcctgtta cccgtgttga ctaaattttg aatgctgctc
    22981 cccataagga gggaatttaa tgaaacgagt cgttccatat cctcttttgt tgatacttgc
    23041 tgtcgttctg gatagagttg caatctcatc ttcccagatc agcattgctc aaagtatacg
    23101 ccccataatt attttaattt tgttggtggg tttaacactc tttctcattc aacgatttgt
    23161 caaagactgg cattatacaa atttcattgt gatgatgata ccaattgcgc tgctcattta
    23221 tcggtctttg tatggttttt tcaagatgaa ttttcctcag caggcagact atttggctct
    23281 tgcgatcctg cctttattgg cagtgacttt cgcggccgtt gtaagtcgtc gagtgtggca
    23341 atggatccgc aatcccgatc agttggcata ttatttcaac ctcgtattta tttttctttt
    23401 agggttccag gggcttcggc tcggcggtcc gatcgttcgg gtgctaagca caggaactca
    23461 gtcacatgct gaagccattt ctactttgac cgatgacgtt caactgcaat ccaaaacacg
    23521 acccgacatt tatgtgatcg tattggatgg ttacggacgc caggatgtcc ttcgagacat
    23581 ttatgattac gacaactctc aatttatcca tcaactggag gaacgtggtt tttacgtcgc
    23641 agccgagaat cacagcaact atgtacaaac tccttatgca atggcatccc tatggaatta
    23701 tgactatctc caaccctggg attcctccac cgattatcca cgctatctgc tggaaccgat
    23761 tcaaaacaac cgcgtttttc ggttgttgaa tgaaatcgga tacacgaccg taagttttga
    23821 aggagacctg gtatttacgg agatcagcga ttccgatgtg tatttatcca actttctgcc
    23881 cctgaataag tttgaaggtt tgttgctggt ggatggtccg tttgaagcgt tatcgaatgt
    23941 ttttggcctg aaattcccca taccaaacaa tgaaacccac cgatctagaa ttcaatacga
    24001 gctggataca cttcaccaaa ttccagaatc tatcgccgga cctaaaattg tatatgcgca
    24061 tattctggcg actcatcccc cgtttgtgct tgatcaggat ggcagtctgg ttcatcagca
    24121 ccaaccctat agcctgtggg atgggagtga gtttaaagat acgcaggatg catattggaa
    24181 cggttaccgg gagcaggtta tcttcaccaa caaagagatc attggcgtag tagacgacat
    24241 tctcgcaaaa tcaaaagcac caccaatcat tctattaatg ggtgatcatg ggccggcttc
    24301 gctgttcaat tggagcttta gtgaccccgg ctgcctgtgg gagcggacca gtaatctgta
    24361 tgccctgcta ttgccaggtc atcaaaacga tggaacggtg tattccacca tcagtcccgt
    24421 aaatagcttc cgggtgattt tcaatacata ctttggcacc gatttcccct tgctggaaga
    24481 taaaactttt ctaacctcct ggagtgaacc atccatcatg atggatgtta cgaataaaac
    24541 agatgtcgtt gccggttgta cgatgcccga tctatcagaa tcaactatgg atcattcgtt
    24601 agatttcctg caaggcgggt tctgaaaagc atttatctgt ctatagttcc caaaagcgga
    24661 aacgctgcag accagcaacg aagagatccc aattcactcc aagaattgtg gagaaagtaa
    24721 ggaccgcgcc ccagaaaaaa ctaccaacga acgggataaa ccaaaacgcc agcagcacaa
    24781 cccaaatcgc cacatcgcgg aagcgatcca cttgcatgcg aatgacggga tgcaggaagg
    24841 gttcaatcgc gttataaccg tcgaagggcg gcaaggggat gagattgaat aacacggctg
    24901 atacctgcag cacgagcagg aaggaaatcc ctggccagat tcccgcctcg gtaacatctg
    24961 aaaaacgaag gatgagccca agaaccatcg cgcataccag gtttgaaaaa ggtcctgcga
    25021 gtgacatcgc gctcaaccag taacgattgc ggatgcgcca gcgctcgatg tacaccgctc
    25081 ccccaggcag accgatcccg cccatgaata gaaacagcaa agggaggacg atggagaaaa
    25141 ccggatgcgt atacttcagg ggattgaaag tgagataacc tttttcgcgt acagtatagt
    25201 caccgccata gtaagcgacg atggcatgag agaactcatg caggcataag gaaaagatcc
    25261 agccaaagaa gacgatgata aaagtcatga tttatcctta gttcgaatcg ggtgcgagca
    25321 tttttgatca gattattcag gatgtgtttt gttccaccca gccgcgtacc actgaatata
    25381 ttcctccaca tgcgctgacc aatactcctc cgtgtgccca ccgctgtaga gatgccattc
    25441 atgcagcaaa ccatattcat tgaattgagc ttcgatattt cctgccatcg tcagctccgg
    25501 gtcattatcg ccgatatcca tgtagacgcg cagctttgag gagtcaggga tatcagccag
    25561 ccagttatca agttgggata catctttttg cgaaatcgca agggaatgca aaccgatcga
    25621 gctgaataaa tcccagcggg tgagaccgag ccgtagtgcc cagcctgcac cgcgcgacat
    25681 cccaccaata gcgcgttgat ctctatcatc cagcgtgcgg taattttcgt caatgtaggg
    25741 gatgatgagc tcaatcagtc tctgcccaaa gcccggaccc ggtggcagat tccaatagcg
    25801 gtcatccggg aacacaacga taaatggcag cgattcgccc gattgaatga gacgatcaat
    25861 aatttccacc gctcccaaac ggacccactg atcctgtgta taggtttggc cgtgcaggag
    25921 atagagcaca ggatagtgct cttccgtttt ctggtcatag catggaggca aatagatcag
    25981 atattcctgc ggcggatttg tcgattcaag cctgccgctg tccacacggc ctgtttccgt
    26041 aaggcaggta agaggagtcg ctgttgccgt atctatgagg ggcgcagttg tctgtgtcgg
    26101 ctgaggcgtt tgcgaggcaa tggtttgaac ttgtgacggc gcaactggcg ggctggcagg
    26161 agtacaagcg tacattgtga gcaatgtgat ggtcgtgagt aatacttttg ttgcttgacg
    26221 ggtattcatc cttcgcatta tactcacagc ctcttaacgg ggcgtggcgc agtccggcta
    26281 gcgcgcttgc tttgggagca agaggtc
//
LOCUS       DASTJP010000556         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_888411, whole genome shotgun sequence.
ACCESSION   DASTJP010000556 DASTJP010000000
VERSION     DASTJP010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888411"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1008)
                     /locus_tag="VFQ23_16280"
     CDS             complement(73..1008)
                     /locus_tag="VFQ23_16280"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908210.1"
                     /translation="MSIAQEFFKKTEALFQAYQEGNYADALTVAEKLAVEYPERDGNT
                     SYWRICLYAVTGQKEHALRALEDALKRGVWWAEEQLRSDPDLASLQNDPQFERLLLLS
                     NEKHKQARAEPELFVYQPDGTGPFPLLIALHGRYGNAERDFSSWKSAIAHGWTLAMPQ
                     SSQPSSSVSFVWDDRQKAMDEIAHHLDSLIEKYPIDNNKIVVAGFSQGAARAIELVMS
                     ERIKANGFLAVVPGPIDLNQLGVWADSSEARGVLISGGRDPRYEMFQQIKAIFSQKEL
                     PLMFENYPEMPHVFPDDFEKVLQKGLEFILQEENE"
     gene            complement(1025..2350)
                     /locus_tag="VFQ23_16285"
     CDS             complement(1025..2350)
                     /locus_tag="VFQ23_16285"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908211.1"
                     /translation="MNRLIKTMLTIVVVILGLWLVLEFGLRWYVESPLTTDFYSSAAR
                     EGLLPRQNEAGVRTVSGSNWIHLGWVADPDNETYRIEKFMDGAWNEIGTAEFGSFLIS
                     DGGGQFRVWRVPKNGSQPEILGEVTADFTGSQSPIFVPRIAGEWKTLFRPETSGSYLN
                     DHTIYQDADGNWRLIGITSKTDGDYSQEKYFAVGVSPDFPPANGMTETEPIADFGELA
                     WAPHVIKDGSKYHIFWSPHQLHQMESNDGIVWENHQVTMPAPYHKFFRDAMVLQVAED
                     QWLLYTTARGNYYSQIDVYQSFDLREWQYIRTALSSGWGSERNSPFSSMESPFVVDYQ
                     GHYYLSLTYNNDSFFLPGLLLPLKIWLNPASYNETLIFHSDNPYDFGAYRGGNNSPTL
                     LTRLETHAPELVHNPDTDSWYITTAGWPWVATLTSGEAAVAPLVWDARP"
     gene            2775..>3134
                     /locus_tag="VFQ23_16290"
     CDS             2775..>3134
                     /locus_tag="VFQ23_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9908212.1"
                     /translation="MITPLHYIIVAFAAVAAGAVNAIAGGGTLITFPMLTFLGIPAVT
                     ANVTNTVALCPGYLGGTLAQWKDMQGQKHRLWLIVPAGIVGGVLGGFLLLQTGERLFR
                     ELVPYLILLASGLLAIQD"
BASE COUNT          759 a          841 c          745 g          789 t
ORIGIN      
        1 cggtttccca catcgcgtag atgcgcggct cggtggattt gaaatcgtac gccttcggta
       61 attcctgttt ggtcattcgt tctcctcttg caaaataaac tctaatcctt tttgcagaac
      121 tttttcaaaa tcatctggga aaacatgcgg catctctggg taattttcaa acatcaaagg
      181 tagttctttt tgagagaata tcgctttgat ctgttgaaac atttcataac gcggatctcg
      241 gccaccagag atcaaaacgc ctcgcgcctc gctcgaatct gcccagacgc ccagctgatt
      301 gagatcaatc ggccccggga cgaccgcgag aaatccgttt gccttgatcc gctcgctcat
      361 caccaactcg atcgcccgcg cggctccctg cgaaaacccc gccacgacaa ttttattgtt
      421 atcaatcgga tatttctcaa tcagtgaatc caaatgatgt gcaatttcat ccatggcttt
      481 ctggcgatca tcccaaacga acgaaaccga cgacgacggc tgtgaggatt gcggcatggc
      541 cagggtccaa ccgtgggcaa tagcagattt ccatgaggaa aaatcacgct cggcgttgcc
      601 ataccgcccg tgcaaggcga tcaataacgg aaaaggacct gtgccatccg gctggtacac
      661 aaacaattca ggttctgctc tcgcctgctt atgtttctcg ttcgaaagga ggagcaaacg
      721 ctcaaactgt ggatcatttt gtaaagaagc caggtccggg tctgaacgta attgctcttc
      781 ggcccaccac acgccgcgct tcagagcatc ttcgagagcc cgtaatgcgt gctctttttg
      841 accggtaaca gcatagagac agatgcgcca ataggatgta ttgccatcac gttcaggata
      901 ttcaaccgcc agcttttcgg cgaccgtaag cgcgtcagcg tagttccctt cctgataagc
      961 ctggaagaga gcctccgttt ttttgaaaaa ctcctgtgca atagacatgt atattcctgg
     1021 gtatttatgg gcgagcatcc cataccaagg gcgcgaccgc cgcttctcct gaggtaagtg
     1081 tcgcaaccca gggccagcct gcagtggtga tgtaccatga atcggtatcg gggttgtgga
     1141 ctaattcagg ggcgtgcgtc tccagcctgg taagaagggt cggcgaatta ttgcctccgc
     1201 gataggcacc aaagtcataa ggattatccg aatgaaagat cagcgtttcg ttataactgg
     1261 ccgggttcag ccagattttg aggggtaaca aaagacctgg caggaagaat gagtcgttgt
     1321 tataggtcag tgagaggtag tagtgtccct gataatccac aacaaacggt gattccatcg
     1381 aggagaaggg cgagttgcgc tcactgcccc agccactgga taatgccgtg cggatgtatt
     1441 gccactcccg caggtcaaac gattgataca catcgatctg tgagtaatag ttgccgcggg
     1501 cagtcgtgta taacagccat tgatcttcag ctacctgcaa caccatcgca tcgcgaaaaa
     1561 acttatgata cggcgcaggc atggtcactt gatggttctc ccaaacgatt ccatcgtttg
     1621 actccatctg atgcaattga tgcggcgacc aaaagatatg atatttgctc ccatccttga
     1681 tcacgtgcgg cgcccatgca agctcgccaa aatctgcaat tggctcagtc tcggtcattc
     1741 cattcgctgg cgggaaatct gggctcacac ctactgcaaa atatttctcc tggctgtagt
     1801 ctccatcagt cttcgatgta attccgatca aacgccagtt gccgtccgca tcctgataaa
     1861 tcgtatggtc gttcaaataa gagccagaag tttcaggacg aaataaagtt ttccattcac
     1921 ccgcgatgcg tgggacgaag attggagatt ggctgccagt gaaatccgcg gtcacctcac
     1981 ccaggatctc cggctggctt ccatttttcg gcaccctcca caccctgaac tgaccacctc
     2041 catcgctgat gagaaacgat ccaaactcag cagtgccgat ttcattccat gcgccgtcca
     2101 tgaatttctc gattcgatac gtctcattat ccgggtcggc aacccagcct aagtgaatcc
     2161 agttcgatcc gctgacagtt ctcacaccgg cttcattctg gcgcggcaat aaaccttcac
     2221 gcgcagcgga agaatagaaa tccgtagtca aaggcgattc gacataccag cgcaggccga
     2281 actccaatac cagccataga cccagaataa ctactacgat ggtaagcatg gttttgatca
     2341 agcgattcac ttgactcctc atagaaacaa aaacacgcct tgtccaacat gaggacgaca
     2401 ggcgtgtcgc ggtaccacct caattcgcta ttagttgtgt agttttttcg tttgatagtt
     2461 ttgtagatcg tggttcaaca caggaactac ccgactatct cactatacaa ctatctaact
     2521 aacaacgctc tctgtggctg acgttcatca gccgatgcta taacgggcaa ccccgcgacg
     2581 gtctactcgg tccgaacctt tcttcgtcaa ttactccagg cgacttcagt gatcgcttcc
     2641 tgtgaaggct tccagccaat ggccttcttc tctatcagtt cgcaaaccac gtactactcc
     2701 cgaatggctg ggattatatg cttgagaata cagcacgtca agaaggtgtc gtgccttttg
     2761 ctataatctt gtttgtgata actccactcc actacattat tgttgcattc gccgcggttg
     2821 ctgcgggcgc ggtcaatgcc attgccggcg gtggcacgct catcacattt cccatgctca
     2881 cgtttttggg catcccagca gtgactgcca atgtgacaaa cacggtcgcg ctttgtcccg
     2941 gctatctcgg cgggactctc gcccaatgga aagatatgca aggacaaaag caccggttgt
     3001 ggttgatcgt cccggccggt atcgtgggtg gtgtgctggg agggtttctc cttttacaaa
     3061 ccggtgaacg attattcagg gagttagtgc catatctcat tctacttgcg tctgggttgc
     3121 tcgcgatcca ggac
//
LOCUS       DASTJP010000557         8656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_180190, whole genome shotgun sequence.
ACCESSION   DASTJP010000557 DASTJP010000000
VERSION     DASTJP010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180190"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..701
                     /locus_tag="VFQ23_16295"
     CDS             318..701
                     /locus_tag="VFQ23_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908213.1"
                     /translation="MTNQSPIKNSQSEEPRRSTWIIGVILIVLGGAFLMQNIGIMPVR
                     FTNWWALFILIPAIGAFEKGLQHYRNANNQITSEASGSFLLGLVLSLVMLGFLFNIGW
                     TYFGPTLIILAGLGILLKSITTNKE"
     gene            1024..1386
                     /locus_tag="VFQ23_16300"
     CDS             1024..1386
                     /locus_tag="VFQ23_16300"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9908214.1"
                     /translation="MQSHTSAGSRHKGKLHPAFFDRAVLVSVLGEIQNREAAMKEIYD
                     ALKPGGMLSITEVVLDPHFQGCEAVLKIVQTVSFKKKKLFGSKWAYTIQLEKSPIQMD
                     SENYTRDRGVLVSFQLLS"
     gene            1411..1803
                     /locus_tag="VFQ23_16305"
     CDS             1411..1803
                     /locus_tag="VFQ23_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908215.1"
                     /translation="MKTITQAGVITPTIIGLALLAATVASMGVTGTKIPLLSNVRMDV
                     ILLVLIGMVICTQGGIGRVAATGAWWHPLSVIGYVLGGAILIITLAVFAEWKLPFIQS
                     DRQALMAIAVLASLKIVNAITHYFLART"
     gene            1826..3019
                     /locus_tag="VFQ23_16310"
     CDS             1826..3019
                     /locus_tag="VFQ23_16310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9908216.1"
                     /translation="MTTVISQLGLKPTALRGETVIVTGAGGGIGYEAARALLWLGANV
                     VIAEINYQNGLKASETLAKEFNCDRVLFVHTNVGDEIDVLKLYKRSVDHFGKVDAVIN
                     NATIAVLGQVKDLPIEDWDRSYRVNLRGPVLMAKYFLPDMLKRNHGVFACVSSTGTAF
                     LGGYETFKAAQVHLANTLDAELEGTNVIAYTIGPGLVLTQTASKAIEQIAHYAGITVD
                     EFFELNKNVMLSAEEAGVGFALSLVFADKFRGQEISSIQALKAADINYGGKDMKIESI
                     DMDFDKRKQAQALCESVRVTLKKQLEDWKRRSLFERQWVVRDFKRSAGMPVEEWLSLL
                     EWLEDALNYGQTSTHPPLEKLASYYEHLAELAKGYEKDPEKLKGSLQQVYRWRDEVKE
                     LEEVL"
     gene            3152..3655
                     /locus_tag="VFQ23_16315"
     CDS             3152..3655
                     /locus_tag="VFQ23_16315"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HET9908217.1"
                     /translation="MSNRILVTYATRTSSTAEVANAVSEVLRTRGYSVDVKSVKAKPS
                     LEGYHGVILGSAIRMGTWLPEMLAFIRSNQTQLNRIPTAIFTVHILNTGSDDTSRAAR
                     DTYTIPVRQLINPVDEAFFAGSIDLTKLSFVDRVLTRLMVGDASPKIGDFREWDRVHA
                     WAKEVQV"
     gene            3702..3896
                     /locus_tag="VFQ23_16320"
     CDS             3702..3896
                     /locus_tag="VFQ23_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908218.1"
                     /translation="MSPEIAYVIPAVFFFFLFLYSLAWVYEDANSHGKTGAAWLLIVW
                     FTWPFGLLVYYFLRNKPVQL"
     gene            4225..5157
                     /locus_tag="VFQ23_16325"
     CDS             4225..5157
                     /locus_tag="VFQ23_16325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9908219.1"
                     /translation="MDNIKLLVGRIALVTGGTSGIGFHTALGLARMGAVVYITGRNIS
                     RGQEAQRQLRAATGHANIHFIRADESTVGGNQELAHRISMETHQLHILINNVGGMYND
                     RWETGDGYEATLAINFVGPFALTDSLLPLLQRSTPARIVNVTSAAYALWQGDPFSDIQ
                     SCEDYSGFYAYSHAKYLNLLWTFALAHRLEDQLEDSWITANAVHPGTAWTNITKRSEP
                     RSFPENLRPLWPVVRLMQRTGSPEKAAHTSIYLASGPELANITGQYFESSIHPKYPGS
                     ALLDQVNQDKAWELAMALVRDAPTALHLESEPVI"
     gene            5193..5741
                     /locus_tag="VFQ23_16330"
     CDS             5193..5741
                     /locus_tag="VFQ23_16330"
                     /inference="COORDINATES: protein motif:HMM:NF039786.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HET9908220.1"
                     /translation="MPPHCDSLSGPVVKMAKKALETGNVQYILPYVPEESENEVITVF
                     DKVLPLHNQPNGAQEVADAFFFETVVRLHRAGEGAPYTGLKPAGLYEGPVIPIAEKSI
                     ETGSIEELKESLTNIIANEVEYRFAHLQFLKQFAEESVKDAREYVEAMLGLQVWSHKI
                     YLAATSGLHNKNGEHHHSIISG"
     gene            5773..6372
                     /locus_tag="VFQ23_16335"
     CDS             5773..6372
                     /locus_tag="VFQ23_16335"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HET9908221.1"
                     /translation="MNRHILVRYATRAGSTLEIAEVIGEVLYARNFIVDIWPVNEKLS
                     IKGYGAIVMGSCIRGGGWLPEMVRFIRENKTQLNKIPIATFTVHVLNRGDDENSRAAR
                     ETYMAPIREMISPVYEAFFAGKVDFSTLSLLDFVSARALQPQVAAHSGDFRDWDKVRS
                     WAQTLFSQKNKIEKSIDFPLACPINRRNKEPSHRMGVQR"
     gene            6662..6832
                     /locus_tag="VFQ23_16340"
     CDS             6662..6832
                     /locus_tag="VFQ23_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908222.1"
                     /translation="MDKKFTFYILLGLVIGAFFGQGIGATNGNAFLGLGIGALIGVCI
                     GWFLSAIDLRQG"
     gene            7059..7652
                     /locus_tag="VFQ23_16345"
     CDS             7059..7652
                     /locus_tag="VFQ23_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908223.1"
                     /translation="MDKSTKTALIIIGVVLLLAAVTSTSILATGLWAFGRFINRAEQT
                     VSESHEVAVRVGAEIADYEVPEGFDSPFSVHIGDVIMIGYKSTSENSHILLAQFPNET
                     SINVDEMLRIISEGSTDPTSIWYNTETTLVEQYPVTIRGQECTLNINEGTSSEGMKYR
                     SAIATFNGRSGPSLVLIASPVDEWDIEMIQSFLASIQ"
     gene            7784..7954
                     /locus_tag="VFQ23_16350"
     CDS             7784..7954
                     /locus_tag="VFQ23_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908224.1"
                     /translation="MDKKFGYYVLLGLLIGSVFGLGYASANGNTFLGLWMGVLIGVFV
                     GWFLAAADLHQR"
     gene            8431..>8656
                     /locus_tag="VFQ23_16355"
     CDS             8431..>8656
                     /locus_tag="VFQ23_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908225.1"
                     /translation="MKNILLGIVRLIALVIVYFVLLTFASSVTTPPEPLQPMKTPQTS
                     QPSALLPVVSLVIILILVYLTHRASWKVVGA"
BASE COUNT         2298 a         2018 c         2119 g         2221 t
ORIGIN      
        1 cgtgctcaac ccctggatgt cactattgcc ggtaagacat accagaacat cagaacagga
       61 acctaccatg actgacgatc agcatattag aaatggtgct cgatagacat gcagacgagt
      121 agggacgcca attaagcagg agtaatggtg aagacgatac ttttggagac acactgaacc
      181 tgtaagaagt gcagcttatc aacttaacca cactgtccga gtggacagtg aagaactccc
      241 ctattgccag gggggttcat ttttctttaa gaatattata gaacctgtcg aaaactaatc
      301 catcgaagga agaaaaaatg actaatcaat cccccatcaa gaacagtcaa tcggaagaac
      361 cgcgtcgcag cacctggatc atcggtgtga tcctgatcgt tttgggcggc gcattcctga
      421 tgcagaacat cggaatcatg ccggtccggt tcacgaactg gtgggcgctc ttcatcttga
      481 tccccgccat cggcgcgttc gaaaagggac tgcaacatta tagaaacgcg aataatcaaa
      541 ttacgtcaga agcaagcggt tccttcttgt taggcctggt tctgagcctt gtcatgctgg
      601 gcttcttatt taacatcgga tggacgtact tcggtcccac gctcataatc ctggctggct
      661 tgggaatcct gctcaaatct ataacgacca ataaggaata atcacaaaga ctactgctgt
      721 gcttctgagc aatatccgag gaaacattat gtccagattt aacttctctc aagaggcccg
      781 cgcaaagata gttctgccgc gatggtaatt ctgtcacggg tgctactagt tctggatgga
      841 tcgttcccct tacctttgcc ttcacctgga tctacaacaa cacatatcgc agcacgctag
      901 ctgtgatcct gttccattcc atggttaact ttagtggcga gctgttcacg ctcaccgaac
      961 gcgccgacaa ctattccatc gtgttgtggt tcgttgcggc agtcagcgtt ataaccattt
     1021 ggaatgcaaa gccatacaag cgctggaagt aggcataaag gaaaactaca tccagcgttt
     1081 tttgaccgag ccgtgctggt gagcgtcctt ggcgaaatcc agaatcgaga ggctgcaatg
     1141 aaggaaatct atgatgcact aaagccgggt ggaatgttat ccattactga agtggtctta
     1201 gacccgcatt tccagggctg cgaggctgtg ctaaagattg tacaaacagt aagtttcaaa
     1261 aagaaaaaac tttttggcag taagtgggct tacaccatcc aattggaaaa atcacctatc
     1321 caaatggaca gtgaaaacta cactcgcgac aggggagttc ttgtttcatt tcaactatta
     1381 tcctaaatgg aaagagacat tatttcacct atgaaaacca taacacaagc tggtgtgatc
     1441 acaccaacta tcatcggatt ggccttgctg gctgccacag ttgcctctat gggtgtcaca
     1501 ggtacgaaga ttcctctact ctccaacgta cggatggatg tgatcctgct ggtccttatc
     1561 ggaatggtaa tctgtacaca gggtggaatt ggtcgagtgg cggcaacagg tgcatggtgg
     1621 catccgctat ccgtcattgg atatgttcta ggtggggcga ttctgatcat tacgctggct
     1681 gttttcgccg aatggaaact tccatttatc caaagcgatc ggcaggcatt aatggcgata
     1741 gcggtccttg cgagtctcaa aattgttaat gcaattacgc actatttcct ggcacgaact
     1801 taaacagccg gatctggagg caattatgac aactgtaata tcccaactcg gtctcaaacc
     1861 cactgccttg cgcggcgaga cagtcatcgt gacgggcgcc ggcggcggga tcggttatga
     1921 ggccgctcgt gccctgctgt ggcttggcgc aaatgtggtc atcgctgaaa tcaattatca
     1981 aaacggattg aaagcatccg aaacactagc caaggaattt aactgtgatc gagtcctgtt
     2041 cgttcatacg aacgttggcg atgaaattga tgtcctgaag ctttataaga gatcggtgga
     2101 tcacttcggc aaagtagatg cagtcatcaa taatgccaca atcgcggtac tgggtcaggt
     2161 caaagatttg cccatcgaag actgggatcg aagttatcga gtcaacttgc gtgggcctgt
     2221 gctgatggcc aagtattttc ttcccgatat gctcaaacga aatcatggtg tgttcgcgtg
     2281 tgtgagttcg acagggaccg catttcttgg cgggtatgaa acgtttaaag ccgcgcaagt
     2341 acatcttgca aatacactag acgccgagct cgaaggcaca aatgtcatcg cctatacgat
     2401 tgggcccggg cttgttctga cacagacagc ttctaaagct atagaacaaa ttgcacacta
     2461 tgcaggcatc acggtcgacg aattttttga actgaataaa aacgttatgc tatcggctga
     2521 agaagccggc gtggggttcg cgctctccct tgtctttgca gataaattcc gcgggcagga
     2581 gatttcatct atacaggctt tgaaagctgc agatatcaac tatggcggca aagacatgaa
     2641 aatcgaatcg atcgacatgg atttcgataa acggaaacag gctcaggctt tgtgcgaatc
     2701 tgtgcgcgtc acgctcaaga aacaattaga agattggaag aggcgttctt tgttcgaacg
     2761 ccaatgggtg gtccgtgact ttaagaggtc cgcaggaatg ccggtcgaag agtggctgtc
     2821 cttactcgaa tggttggagg atgccttgaa ttacggccaa accagcacgc atccgcctct
     2881 tgaaaaactc gcgagctatt atgagcattt agcggaattg gcaaagggtt acgaaaagga
     2941 tcccgagaaa ttgaagggaa gccttcagca ggtgtatagg tggcgcgatg aagtgaagga
     3001 gcttgaggag gttttgtgat acctatccac gtgactagtt aaaaacctcc taagggttgg
     3061 gggatttttt tgatgctgct cagtaaacta gaaatggaaa aacccagttt gcttttggtg
     3121 gtatcccaaa tgcaaggaaa aggagaatcc aatgagcaac cgcattcttg tcacctatgc
     3181 cactcggacc agctctaccg ctgaggtagc gaacgctgtg agtgaagtgc taagaactcg
     3241 cggatattcc gtggacgtga aatcagtaaa ggcaaaacca tctctggaag gatatcacgg
     3301 agtcatcctc ggaagcgcca tccgcatggg aacgtggcta cctgaaatgc tagcctttat
     3361 cagatccaac cagacacagc tgaatcgcat ccctacggcg atctttacgg ttcacattct
     3421 taacacaggc agcgatgata ccagtcgtgc agcaagggat acctatacca ttccggtgcg
     3481 gcaactgatc aacccggtgg atgaagcctt cttcgccggg agtattgacc ttaccaaact
     3541 ttcttttgtg gatcgtgtac ttaccagact catggtcggt gatgccagtc cgaagattgg
     3601 agatttccgc gagtgggaca gggtgcacgc ctgggcaaaa gaagtgcaag tttaggacgc
     3661 aaaaattaac tacacatcat ttctgaaaat aggaggttgc tatgtctccc gaaatagctt
     3721 atgtgattcc ggccgtgttt ttcttcttcc tgtttctata tagcctggca tgggtatatg
     3781 aggacgcgaa ttcccatggc aagaccggtg ctgcatggct tttgattgtc tggttcacgt
     3841 ggccctttgg cctgctggtc tattactttc ttcgcaataa gccggttcaa ctttagaaga
     3901 atcatgtgaa gcctgcacgt agcatatttt tgttcggtct catgctgacg aaagctgcta
     3961 gccaacaagc ttattacacg atccgattcc tggtagatcc ccaacgggta aatgatacat
     4021 atcggtctta tgcctatttc tgttgggcgg atgatgttat ggctgtccaa atcaaattct
     4081 gaaaccagat tttggttatg gattgatgcg aatgatgtga cggagctttg gattttacag
     4141 catcaattag gattcacgag ctaaagccac aatcagccca acgattgtga attgcagttt
     4201 cttcgtgacc ctaaggagct agaaatggac aacattaaat tgctcgttgg aagaatcgcc
     4261 ctggtcactg gcggaacatc aggtattggc ttccacacag cgcttggact cgctcgaatg
     4321 ggggccgtcg tttatataac cgggcggaac ataagccgag ggcaggaggc acaacgtcag
     4381 ctgcgagccg ctacgggcca cgcgaatata cactttatcc gagccgatga atctacggtt
     4441 ggagggaacc aggaacttgc gcaccgaatt tcaatggaga ctcaccagct tcacatccta
     4501 attaacaatg tgggtggcat gtataacgac cgctgggaaa caggcgatgg ctatgaagca
     4561 acattggcca ttaactttgt ggggcctttc gcgctcaccg actcactttt gccattgttg
     4621 caacgaagca ctccggcccg tattgtcaac gtgacctcgg ctgcgtatgc cctatggcag
     4681 ggtgatccat tttcagatat ccaatcgtgt gaagattatt ctggtttcta tgcctacagc
     4741 cacgcaaaat acctgaacct gctttggact tttgcactcg cgcacaggct ggaggatcag
     4801 ctggaagata gttggatcac agcgaatgct gtccacccgg gtacagcctg gacaaacatt
     4861 acgaaacgaa gtgagccgcg tagttttcct gaaaatttac ggcccctctg gccggttgta
     4921 cgcctgatgc agcgcaccgg ttcgccagaa aaagctgcgc acacctcgat ctatcttgca
     4981 agtggaccag aactagcaaa tataactggt caatactttg agagcagcat acacccaaaa
     5041 tacccgggtt cagcacttct tgatcaggtt aaccaggata aggcctggga actggcaatg
     5101 gcgctagttc gggatgcacc tactgcgttg catctcgagt ctgagccggt tatttaagga
     5161 aaaagctgca gatgcttaga aaggaaattc ctatgcctcc acattgcgac tcacttagtg
     5221 gaccggtcgt aaaaatggcg aagaaggccc tcgaaaccgg taatgtccaa tacattcttc
     5281 catacgtgcc agaagaaagc gaaaacgaag tcatcacagt atttgataag gtattaccac
     5341 tccataacca gccgaacggc gcacaggaag ttgcagatgc attcttcttt gagacggtgg
     5401 tccgtttgca ccgcgccggc gagggcgcgc cctatacggg acttaagcct gcaggactgt
     5461 acgagggacc tgtcattccc attgcagaga aatccattga aacgggttcg atcgaagaat
     5521 tgaaagagtc actcacaaat atcatcgcca acgaagtgga gtaccgtttt gcccatcttc
     5581 agtttttaaa acaatttgcc gaagaaagcg tcaaagacgc gcgcgagtac gtagaggcca
     5641 tgctggggct tcaagtgtgg tctcacaaaa tctatctcgc cgccacatcc ggcctgcata
     5701 acaaaaacgg cgagcaccat cacagtatca tttcgggttg aaaccgcgga ttgtgcacat
     5761 cggagcgagc ttatgaatag acatattctc gttagatatg cgacgcgtgc aggttccact
     5821 cttgaaattg cagaggtcat cggcgaagtg ctgtatgcgc gcaatttcat agtagacatc
     5881 tggcctgtaa atgaaaaact gtcaattaag ggatacggtg ctattgttat ggggagttgc
     5941 atacgcgggg gtggatggtt gcccgaaatg gttcgattca ttagggagaa caaaacgcaa
     6001 ctaaataaga tccctatagc aacattcact gtgcatgtcc tcaacagagg cgacgatgaa
     6061 aacagccgcg cggcccgtga aacctatatg gctccgattc gggaaatgat cagtcctgta
     6121 tatgaggcct tcttcgcagg gaaagtcgat ttctctacac tgtctctcct cgactttgta
     6181 agtgccagag ctttacaacc gcaagtcgcc gcgcattcag gtgacttccg tgattgggac
     6241 aaagtccgca gctgggcaca aacactcttt tctcagaaaa acaaaattga aaaatcaatc
     6301 gactttccgc tggcttgtcc tattaatcgc cgtaataaag aaccaagcca tcgcatggga
     6361 gtccagcgct aacgtggtaa agagaattcc tgtcagcttg gggtttatga ccctcttact
     6421 cgtcttggct ctgctcaacc tggtatctgc agcttgttgt ttaaaaaaat ccacattgga
     6481 atcatactgc cggaacttaa actatccgtt tgactagtca aaaccattct attggcagag
     6541 gaatgtttgt tcacccagga ctatattcaa tattgcgaag aaatccttct gtctttgcta
     6601 tgttaaaagt gacagagaaa atatgcataa atgaacaaaa agaaacaaaa ggagattcaa
     6661 aatggacaag aaattcactt tctatatttt actgggcttg gtaatcggtg ctttctttgg
     6721 gcagggcatc ggggccacga acgggaacgc ctttcttggt ctcgggatcg gtgctctcat
     6781 cggcgtgtgc attggctggt ttctttcagc aatcgatctt cggcaaggct gatcaacctt
     6841 catcaaacaa tcgaccctga aggtttggat tcagaaccat cagctttatc attctcctgc
     6901 ctgttcttga gatgatgctt tagttgatct tcgaaggaat ctaccttcgt ccgggtgagc
     6961 ctgtcccatt cgtgttgggc aattgtttgc cgtggttcct acaaatagtc ttcaaggtag
     7021 aggatataaa agttggatca gtagtaaagg agtttcatat ggataaatca accaaaacgg
     7081 ccttgatcat cattggtgtc gtcttgctgc tggctgctgt tacttcaaca agtattcttg
     7141 ccacaggctt atgggcattt ggtagattta tcaaccgggc tgagcaaacc gtcagtgaaa
     7201 gtcatgaggt ggcagtccgc gttggagctg agattgcaga ctatgaggtc cctgaaggat
     7261 tcgactctcc gttcagcgtt cacattggag atgtgatcat gattggttac aagtcaacga
     7321 gtgagaactc tcatatcctg ctggcacaat tccccaatga gacaagcatc aatgtcgatg
     7381 agatgttgag gattatcagt gaaggttcca ccgatccgac cagcatttgg tacaacactg
     7441 aaacgactct ggtcgagcag tatcctgtca ccattcgcgg tcaggaatgt accctaaata
     7501 tcaacgaagg cacatcttca gaaggaatga agtatcgcag tgccatcgcg accttcaatg
     7561 gcagaagtgg tccctcgtta gtcttgatcg cgagcccggt agacgaatgg gatatcgaaa
     7621 tgatccaatc cttccttgcc tccattcaat gagctaaagc ttaccctatc cacttgcata
     7681 gttccaaact cccccgctgg caggggagtt tttttcttag cgaacctatt atggaatcgg
     7741 taatagctgt atcttcgtat tggatcctcg taggagattt gagatggata aaaaatttgg
     7801 ctactatgtt ctactgggct tgctaattgg cagcgtattt ggactaggct acgcctcagc
     7861 aaacgggaat accttccttg ggctgtggat gggcgttctt attggcgtat tcgttggttg
     7921 gtttctcgcg gcagctgatc ttcaccaacg ttgatcctat tcaagcaaga gttcaccgct
     7981 ctcctggaga gggaataccg acaatttccc cagcgcgttt gattgaaacc tgaagaattt
     8041 gatattccac ccatcctcaa cttctaacga taatgtcacg gctcagttag gtgaatcaac
     8101 tggatcttat tacactccgc cagatccata atcgttccct gcctggagga tacgatgtcg
     8161 cgacggccac gaaatttagg ctgagtcagc gcttttcgct tcaatctcgg actcaccccc
     8221 tatacagact atccaagtgg atagtccaaa actcatccac agacagggga gttttttttc
     8281 gtttctatat agagtaaagc aaaggaaact attaatacca tctacttata agcccagctg
     8341 actttgagtc gccgggggaa tgtaagctgc ccatcgaacc attcattgaa gcgcgcggtg
     8401 attgatgcca tactggaaag cgaggtcgaa atgaaaaaca ttcttcttgg catcgtgcgt
     8461 ctgattgcac tggtgatcgt ctattttgtc ttacttactt tcgcgtcgag cgtaactacc
     8521 ccgcctgaac ctttgcagcc tatgaaaacg ccgcaaacga gtcagccttc cgccttgttg
     8581 ccggtcgtga gcctggtcat aatccttata cttgtttatc tcacccaccg ggcgagctgg
     8641 aaggtggtag gggcat
//
LOCUS       DASTJP010000558         4677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_561088, whole genome shotgun sequence.
ACCESSION   DASTJP010000558 DASTJP010000000
VERSION     DASTJP010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561088"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VFQ23_16360"
     CDS             <1..1333
                     /locus_tag="VFQ23_16360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9908226.1"
                     /translation="VQRASELNLLKGQERVAQSKKVKRSPVRYVSNDGLHIAYQTIGE
                     GSIDLLFIHGFLSNLELAWENPEYTEFFEQLGSFARVILFDKRGMGLSDRVQGAPSLE
                     NTLSDAMCVLNACGSNRTFVLGTSEGGAAAVLLASTYPKRVHGLILYASTPKLVKTNG
                     EPAWADDEDQFNSSIEQIQKQWGGPWAIQNFAPSRAQDEAFREWWAKALRSSTSPSEA
                     SAVLRTVREIDIRPLLPQIHTRTLVLHKTQDRMLTVEAGRYLATHMPNAQLVELPGAD
                     HFFFVDSAQVIEAIRGFCSQTDETPADTMIGIVLYVYLQNMKREEKAIELRLKEFRAK
                     GIFFANDEATSVFDSPSRAIQCALKLRESITNDSLRISLHVGECNTANGKPSSSVVEA
                     ARRASEFGPQGKIIVTQTLRDILAGSGAVFDLRRIHLDQQKQESISFYALA"
     gene            1360..1800
                     /locus_tag="VFQ23_16365"
     CDS             1360..1800
                     /locus_tag="VFQ23_16365"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET9908227.1"
                     /translation="MQNNSASIIGAWRLIAFEFRKADGNIIYPFGEKARGSIIYTETG
                     RYSAQLMRVDRPRFSSGDQMQGTVEEMEANYKGCISYFGTYDINPQDSLIIHHVEGSI
                     FPNMEGRDQERFFELSEHRLQLTTPPIKLDGVKTVGVLLWERIG"
     gene            1822..2481
                     /locus_tag="VFQ23_16370"
     CDS             1822..2481
                     /locus_tag="VFQ23_16370"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET9908228.1"
                     /translation="MQLISVNLGQERTLQRNDRVEQTGIFKFPTDEPVRVTALGLEND
                     VIVSKKHHGGPDQAVYVYGGADYQWWSRELGKEILPGTFGENLTISELESATFNVGDY
                     IHIGEVTLQVTAPRTPCSTFATRMEDPQWVRKFRYAERPGLYCRILTEGIVQAGDSVS
                     VEKYTGETISILQMYRDYYDKNKSEEVLRLHLNAPVAIRVRRALERELENLLLDRLPK
                     S"
     gene            2542..3369
                     /locus_tag="VFQ23_16375"
     CDS             2542..3369
                     /locus_tag="VFQ23_16375"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HET9908229.1"
                     /translation="MIFQVYVPTFPLNRFIERLFYYEGINAVHAMDRFLPDGNTEIII
                     DLTEGTQFIYDNETLEEIQTCRYFWASGVRTQPITIPSGNGTRMLVIAFKKGRAYPFY
                     PMPMNELTDHVVSGDLIFKRAILELREQLLSAASIEQMFLLVENFLFQRSGDLLSPEY
                     ASRCIEYAVTAIINGPNRLDFQELSDQIGYSQKHFISIFKGQVGVSPKQYLRIMRFQH
                     AVLEMEACQPIDWRHFSRENGYYDQSHFIHDFKYFSGFTPSEYMQRKTDVVNYVPVG"
     gene            3452..3853
                     /locus_tag="VFQ23_16380"
     CDS             3452..3853
                     /locus_tag="VFQ23_16380"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HET9908230.1"
                     /translation="MENINHLAVFVCAIANLALGALWYSPALFYRAWAKETNLTEDQL
                     AKSNPAKTYGLAFLFSLIISYNLAFFLSDANTTPAWGATAGFLAGFGWSTLIFAIIAL
                     FEQRSWRYILINGGFITVYFTLIGFILGIWR"
     gene            3873..4442
                     /locus_tag="VFQ23_16385"
     CDS             3873..4442
                     /locus_tag="VFQ23_16385"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HET9908231.1"
                     /translation="MDTLDKAVQTQLNNIQKKTGMSLAELSNLVKKSGLSKHGEIRDM
                     LKQKLGLGHGDANLIVHTVFQSDGTSAAKGKSTDAVLDEIYVGAKAHMRPIHEALMKQ
                     INKFGEFEIVPKKGYVSLRRKKQFAMIGPKTNTRFEVGMNAKDFKKDARLLEQPKGSM
                     CNYIVNLTDAKEVDDELIGWIKSAYEGAG"
     gene            4448..>4677
                     /locus_tag="VFQ23_16390"
     CDS             4448..>4677
                     /locus_tag="VFQ23_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908232.1"
                     /translation="MSIEQGFANNLFQLMEETFESKHHGIYLDEGTSLFETLETVSAE
                     EASIPVGGKCASLAAQVAHVTFFIESFERFAL"
BASE COUNT         1255 a         1108 c         1170 g         1144 t
ORIGIN      
        1 cgtccaacgc gcctcggaac tgaacctgct caaaggtcag gaaagagtcg ctcagtccaa
       61 aaaagtgaaa cgctcgccgg ttcgatacgt ctccaacgac ggactccata tcgcttatca
      121 aaccatcggt gagggttcca ttgatctgtt gtttatccat ggctttcttt ctaatcttga
      181 gctcgcctgg gagaatcctg aatataccga attctttgaa caactcggct cgtttgcacg
      241 ggtgattctg tttgacaaac gtggaatggg actttctgac cgcgtgcagg gcgcaccatc
      301 actggagaac acgctgagcg acgcgatgtg tgtgctcaat gcctgcggtt ccaaccggac
      361 ctttgtcctg ggcacatccg aaggaggcgc cgcggccgtt ttgctggcct ccacctatcc
      421 taaacgcgtc catggactga tcctctacgc atccacgccg aagctggtaa agacgaacgg
      481 tgagccagcg tgggcagatg acgaagacca gttcaactcc tctatcgagc agattcaaaa
      541 acaatggggc ggcccgtggg cgattcagaa cttcgctccc tcccgtgccc aggatgaggc
      601 tttccgcgaa tggtgggcga aggcattgag atcatccacc agcccatcag aagccagcgc
      661 agtgttgaga accgtgcgtg agattgacat tcgtccgctg ctgccgcaaa tccacacacg
      721 gacgctggtg ctccacaaaa cgcaggatcg catgctcacc gtggaggcag gtcgttatct
      781 cgcaacgcac atgcccaacg cacagctggt tgaactgccg ggtgcagacc acttcttctt
      841 cgtagacagc gcacaggtca tcgaggccat tcgaggcttc tgcagccaga cagatgagac
      901 acctgccgat acgatgattg ggattgtctt gtatgtgtat ctgcaaaata tgaaacggga
      961 agaaaaagcg attgaattaa gattgaagga atttcgcgcg aaaggaatct ttttcgcgaa
     1021 cgatgaggcg acctctgtat ttgatagtcc gagccgtgca attcagtgtg ccctgaaatt
     1081 gagagaatcc atcacaaacg attcgctccg tatcagcctg cacgtaggcg aatgcaacac
     1141 agcaaatggc aaaccttcat cctctgttgt ggaggcggca cggcgcgctt ctgaatttgg
     1201 tccacagggc aaaattattg tcactcaaac cttgcgtgac attctggcag gttcaggtgc
     1261 ggtattcgat ctgcggcgaa tccatcttga tcaacaaaaa caggaaagca tttcctttta
     1321 tgcgctcgca taagcgttca agacgattgg atatgtccta tgcaaaacaa ttcagcttct
     1381 atcatcggtg cgtggaggct tatcgccttc gagttccgca aagcggatgg aaatatcatc
     1441 tatccatttg gcgaaaaggc gcggggctcc atcatttaca cggaaaccgg tcgctattcc
     1501 gcgcaactga tgcgagtcga ccgtccgaga ttttcgtcgg gtgatcagat gcaaggtaca
     1561 gtggaggaga tggaagccaa ttacaaaggc tgcatttcct atttcggcac gtatgatatt
     1621 aaccctcagg atagtcttat cattcatcac gtggaaggtt ccattttccc gaatatggaa
     1681 gggcgagatc aggaacggtt ctttgaactc tcagagcatc gcctgcaact aacgaccccg
     1741 cccatcaaat tagatggagt gaagacagtc ggcgtcttat tatgggaacg aattgggtaa
     1801 gctatgaaag agaagaaaac tatgcaattg attagtgtca atcttgggca ggagcggacg
     1861 cttcagcgaa atgaccgtgt cgaacaaaca gggattttta aatttcccac agatgaacct
     1921 gtaagagtga cggcacttgg gctggaaaat gatgttatcg tcagcaaaaa acatcatgga
     1981 ggtccggatc aggcagtcta tgtgtatggc ggcgcggatt atcagtggtg gtcgcgggag
     2041 ttaggaaaag agattcttcc cggcaccttc ggtgaaaacc tgaccatcag cgagctggaa
     2101 agcgcaacgt ttaatgtcgg tgattacatt catatcggtg aggtgacctt acaagtcact
     2161 gcgccgcgca cgccatgttc aacctttgcc acacggatgg aagatccgca gtgggtcagg
     2221 aaatttcgct acgcggaaag acctggccta tactgccgta ttcttacgga gggaatcgtg
     2281 caggcgggtg attcagtttc cgttgaaaaa tacaccggcg agacaatttc aattttgcag
     2341 atgtatcgcg attattacga taaaaacaaa agcgaagagg tgctacgcct tcaccttaat
     2401 gcaccggttg caatccgggt gcgaagggca ttggagagag aattggaaaa tttattgcta
     2461 gatagacttc cgaagtcttg aagacttcgg aagtctgatc cgttgttgcg aatctagaaa
     2521 ctatgttcta taatcaatcc catgatcttt caagtctatg ttcccacttt cccactgaac
     2581 aggttcattg aaagattatt ctattatgag gggataaacg ctgtgcatgc catggaccga
     2641 ttcctgcctg atggcaatac tgaaatcatc atcgacctca cagaaggaac acaatttatt
     2701 tacgataacg aaacactgga ggagatccag acttgccgtt atttctgggc aagcggtgta
     2761 cggacccagc ctatcaccat accgtctggc aacggcactc ggatgttggt gatagcattt
     2821 aaaaaaggga gagcctatcc cttctaccca atgccgatga atgaattgac agatcatgtg
     2881 gtgagtggag atttgatctt caagagggca attcttgaat tgagagaaca attgctctcc
     2941 gccgcgtcta ttgagcagat gtttctactt gttgaaaatt ttctgtttca acgttccggg
     3001 gacttacttt ccccggaata tgcctcaagg tgcattgaat acgccgtcac agccattatc
     3061 aacggaccga accggctgga ttttcaagaa ctcagcgatc aaatcgggta ttcacagaag
     3121 catttcataa gcatattcaa aggacaggtg ggcgtctccc caaaacaata tttaagaatc
     3181 atgcgctttc aacatgccgt tcttgaaatg gaagcctgcc agccgattga ctggcgtcat
     3241 ttctctcgag aaaatggata ttacgaccag tcgcatttca tccacgactt caagtatttt
     3301 tccggcttca cgcccagcga gtatatgcaa agaaaaacgg atgtggtgaa ttacgtgccc
     3361 gtgggttaag gtaaattttt tccaataccg aaaatttcgt ctcgtgtagg attatgttca
     3421 aatcattcta cttccatcaa ggagacgaat catggaaaat atcaatcacc tcgctgtctt
     3481 tgtctgtgca attgccaatt tagcattggg cgcgttatgg tattcgccag cgctgttcta
     3541 cagagcctgg gcaaaggaaa cgaatctaac agaggatcaa ctcgccaaat cgaacccagc
     3601 gaaaacctat ggactggcat tcctgttttc gctcattatt tcctataacc tggcgttctt
     3661 tctgagcgat gcaaatacga ctcctgcctg gggcgcgacg gcaggcttcc tggctgggtt
     3721 tggctggtcc acgctgatct ttgccatcat cgccttgttc gaacagcgtt cgtggagata
     3781 catcctaatc aatggcggtt tcatcacggt gtacttcaca ctcatcggtt ttatcctcgg
     3841 aatttggcgt tagaatcaaa aggaaataaa caatggatac cctagacaaa gctgtccaaa
     3901 cacaactcaa taatattcaa aagaaaaccg ggatgtccct ggctgagtta tctaatctcg
     3961 tcaagaaaag cgggttgagc aaacacggtg agatccgcga tatgctcaag caaaagcttg
     4021 ggcttggtca tggggatgca aatctgatcg tgcatacggt ctttcaatcg gatggcacga
     4081 gcgctgcaaa aggcaaatct accgatgcag tgctagatga aatctatgtc ggtgccaagg
     4141 cacatatgcg ccccatccac gaggcattga tgaagcagat caacaaattt ggtgagttcg
     4201 agatcgtgcc gaagaagggc tacgtcagcc tacgccgcaa gaaacaattc gcgatgatcg
     4261 gaccgaagac caacacacgc ttcgaggtcg ggatgaatgc caaagacttt aagaaagacg
     4321 cgcggctgct ggaacagcca aaaggcagta tgtgcaatta catcgtcaat ctaaccgacg
     4381 cgaaggaagt ggatgacgag ttgataggtt ggatcaaatc cgcttatgaa ggagctggct
     4441 gatacatatg tccattgaac agggttttgc caataattta ttccaattga tggaagagac
     4501 cttcgaatcc aaacatcacg gtatttacct ggatgaaggc acttctcttt tcgagacgct
     4561 tgaaacggtt tccgcagaag aggcttcgat ccccgttgga ggcaagtgcg cctcgctggc
     4621 tgctcaggta gcgcatgtga cgttcttcat cgagtcgttc gagcgatttg cgctaca
//
LOCUS       DASTJP010000559         4233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1311241, whole genome shotgun sequence.
ACCESSION   DASTJP010000559 DASTJP010000000
VERSION     DASTJP010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311241"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFQ23_16395"
     CDS             <1..496
                     /locus_tag="VFQ23_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9908233.1"
                     /translation="IDVGKGILHWKLRNDPRVVVMEETNARFVESLPEPVSLVTVDAS
                     FISLKILLPVVQKWIFPSPDPSGRPLPAGEGGILALIKPQFEAGKKDVARGDGVIRDP
                     EIHKQVLLDVLSFAQQTGFRVRGLIKSPLLGPKGNVEFLVWLSQAQDVEEREELGPII
                     ETVL"
     gene            541..1170
                     /locus_tag="VFQ23_16400"
     CDS             541..1170
                     /locus_tag="VFQ23_16400"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET9908234.1"
                     /translation="MPPLTDTEFNIHYPQPLLIVISGPSGAGKDTVMQRMKERGLPFH
                     FVVTATTRPKRANEEHGKDYLFISNEEFARMIDEDELIEHAIVYGDYKGIPKQQVREA
                     LASGMDVVMRLDVQGAETVRKLAPEALMIFITTESESALVHRLETRKTETANSLAIRI
                     ATARKELNRVEAFDYVIVNRNFHLDETVDIIRAIIDAEHHRVKPRKVTL"
     gene            1167..1937
                     /locus_tag="VFQ23_16405"
     CDS             1167..1937
                     /locus_tag="VFQ23_16405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9908235.1"
                     /translation="MNDDPFRSRSQSLNSDQTAPEPQPEPQAVRVAAPEAAPYVTYAI
                     IAVTSLIYVLQLATSDLPIVFLAKDNDLIRRGEFWRLLTPALVHGSLPHILFNMYALL
                     AFGTSLERHFGHGRFFLLYVLGAFAGNVMSFLLTQADSVGASTAIFALIGAEGVFLVQ
                     NRKLFAGQFRNAIGNVIFVVAVNLFLGLAPTIDNWGHIGGLLGGLIFASFAGPLWEIE
                     GINPTYRFVDQREFGAVLIGLVTVLFIFGGLAFWGFVR"
     gene            1962..2543
                     /locus_tag="VFQ23_16410"
     CDS             1962..2543
                     /locus_tag="VFQ23_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908236.1"
                     /translation="MWSTIQIVFVVLGLLLAYGGDRLSMPILFYGGMACFGLAAMAIG
                     AEGIFTQRIVLGRRRRRTTTTYTGIPAVLQGVQFSLIGLFFIAISGLLYLNIGRDIFV
                     QFVRRPGFPLVILGSACLLQAVIAIMGSHEIKQGPRWVVILSLFTARLYPGLLLIVIG
                     LAAIGAGLFEIAAPAAFDEMGGRALEELYGLNR"
     gene            complement(2894..3124)
                     /locus_tag="VFQ23_16415"
     CDS             complement(2894..3124)
                     /locus_tag="VFQ23_16415"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET9908237.1"
                     /translation="MERIIVDPQIHFGKPVVKGTRITVQNVLELVNEGLSFDQIMQDY
                     YPDLKKADIHACLQYAIALVAAEDIHISSVAA"
     gene            complement(3271..>4233)
                     /locus_tag="VFQ23_16420"
     CDS             complement(3271..>4233)
                     /locus_tag="VFQ23_16420"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908238.1"
                     /translation="PGENIRTKGSAIYTTSVVSFALAQLDAANQVLFNAVRYLAAHRN
                     TNGLWNSGHDNAWAMIALNEAMVGFGDLNADFTFNATFNGGPLANGDVAGNQVLTPVK
                     SLVPIDLLSATSPNLLTITRDDGLGRLYYNATLKVNRPVEDVQPLDAGMQIERVYCES
                     ATRQAARSPGSQTARGCTPLSTFQLDSDQPVTAQLTLTLPHDAYYVMVEDFIPAGTEI
                     LNRALKTSQQGIDSTEVQVEPQFEDKNPFSEGWGWWLFNSPQIRDDGILFTSDYLPAG
                     TYILTYTLIPLQAGEFRVLPAHAWQSFFPEVQGTSAGAVVEIAP"
BASE COUNT          987 a         1006 c         1147 g         1093 t
ORIGIN      
        1 gatcgatgtg ggcaagggaa ttctgcattg gaagttacgg aacgatccgc gcgtggtagt
       61 gatggaagag accaacgcac ggtttgtaga gtctttgccg gagccggtct cgcttgtgac
      121 tgtggatgcc tcgttcattt cgttgaagat attgttgccg gtggtacaaa aatggatttt
      181 tccctcaccc gacccttcgg gtcgccctct cccagcggga gagggaggta tcctcgccct
      241 catcaaacct caatttgaag ctgggaaaaa agatgtggcg cgcggcgatg gtgtgatacg
      301 cgatcctgag attcataagc aggtgttgct ggacgttttg agttttgctc aacagaccgg
      361 tttccgtgtg cggggattga ttaaaagtcc gctgctgggg ccgaagggaa atgtagagtt
      421 cctggtctgg ttaagccagg cacaggacgt tgaagagcgc gaagaattag gtccaatcat
      481 cgaaacggtg ttgtaaccaa gccaaattta tctggtgagc ccgtctggta aaattccttc
      541 atgcctccac ttaccgatac cgaattcaat attcattatc cacaaccgtt gctgatcgtc
      601 atttccggtc catcgggagc gggcaaagat accgtgatgc agcgtatgaa ggaacgcggg
      661 ctaccctttc attttgtggt aactgccacc acacgaccaa agcgggcgaa cgaagaacat
      721 ggcaaagatt atttatttat ctcgaatgaa gagtttgcac gcatgatcga tgaggatgaa
      781 ttgatcgaac atgcaattgt ctacggcgat tacaaaggga tacccaaaca gcaggtccgt
      841 gaggcgctgg ccagcggcat ggatgtggtc atgcgtctgg atgtacaggg cgcggagacc
      901 gtccgcaagc tggcgccgga agcattgatg atctttatca ccaccgaaag cgaaagtgcg
      961 cttgtgcatc gccttgaaac gcgtaaaacc gagactgcga attcattggc cattcgcatt
     1021 gctacggcgc gcaaagaact caatcgagtg gaagcgttcg attatgtgat cgtcaaccgc
     1081 aattttcatc tggatgaaac cgtggatatc attcgtgcga tcattgacgc ggaacatcac
     1141 cgcgtgaaac caagaaaggt gactctgtga acgacgaccc atttcgctcc cgatcccagt
     1201 ctcttaatag cgatcaaacg gcgccagaac cgcagccaga accgcaggca gttcgtgtcg
     1261 ctgctcctga agctgcgcct tatgtaacct atgccattat tgcggttaca agtttgattt
     1321 acgtcctgca actggctacc tctgacttgc cgattgtttt tcttgcaaaa gataatgacc
     1381 tgatccggcg cggcgagttc tggcgattgc ttactcctgc tctggtgcat ggatcgcttc
     1441 cacacatttt gttcaatatg tatgcccttc ttgcgtttgg gacaagcctc gaacgtcact
     1501 ttgggcatgg acgctttttt ttactttacg ttttaggtgc cttcgcagga aacgtaatgt
     1561 cattcctttt gacccaagcc gactctgtag gcgcatctac cgcgatcttc gccttgatcg
     1621 gtgccgaagg cgtctttctg gttcaaaacc gaaaattatt tgcgggtcaa ttccggaacg
     1681 cgatcgggaa tgtgatcttc gtcgtggcgg tcaacctgtt ccttgggctc gcaccgacca
     1741 ttgacaactg ggggcatatc ggaggcctac tcggaggatt gatctttgct tcgtttgccg
     1801 gaccgctatg ggaaattgag gggattaatc ccacgtatcg cttcgtggac cagcgcgagt
     1861 ttggtgctgt gctgatcgga ctggtgaccg tgttatttat ttttggcggg ctggcgttct
     1921 ggggatttgt acgataaaga gagatcagga ttcgatcatc catgtggtca acgattcaaa
     1981 tcgtattcgt cgtgcttggt ttgctgcttg cctatggcgg ggaccgactt tccatgccga
     2041 ttctctttta tggcggcatg gcgtgttttg ggctggctgc catggcaatc ggagcggaag
     2101 gtatcttcac ccagcgtatc gtattgggca gacgtcgtcg tcggaccaca accacatata
     2161 cgggcatccc ggcggttctg caaggtgtgc agttttcctt aatcggcctg tttttcatcg
     2221 cgatttccgg gctgctgtat ttgaacatcg gccgtgatat ctttgtgcaa tttgtgcgcc
     2281 gcccgggttt tccgctcgta atcttgggct cagcatgcct gttacaagcg gtgattgcga
     2341 ttatgggctc tcatgaaata aagcagggtc cgagatgggt ggtgatcctg agcctgttca
     2401 ctgcccggct gtatccggga ctcttattga tagtgattgg cctggcagcg ataggcgcag
     2461 gtttatttga aatcgccgcg ccggcagcgt tcgacgaaat gggtgggaga gcgttggaag
     2521 agttgtacgg gttgaataga tagttataaa ttcacggcac aaaagatcaa cacaggttca
     2581 tacaagcttt ttagggttag tatagagtat tagagactgg agattagggc ggagtatttt
     2641 tgttcaagct ggcagatccg agttaaacct atttccgatt gttccacgta cggcaaccac
     2701 acccaattac caaggaaacg tggacccccg ctttacggat gttggtccgc aagaaacgcc
     2761 cacgctgcgg cctacaatag atcagagtct ggtgcttgcg ataaactcag ttctgaggca
     2821 gttactatgt catgaccctg ctcacgtaag aatcgttcgg tgaaagcata cacatcttga
     2881 tcgagcaaaa acttcatgcc gccaccgagg agatatgaat atcctcagcc gccaccaaag
     2941 cgatagcata ttgaaggcag gcatgaatgt cagctttctt caaatcgggg taataatcct
     3001 gcatgatctg atcgaatgac agaccttcgt tgacaagttc aagaacattt tgcacagtaa
     3061 tgcgtgtccc tttgacgacg ggcttgccaa agtgaatttg gggatcaaca attatccgtt
     3121 ccataggtca cctcctattg attctatcat cacattttca tacttgctta ttgatttttc
     3181 gtgacagaat tgatgatgcc gaacagttgc cactggtggg caagcactta atatcaaaac
     3241 cccaataaaa gtaatctttt taattcatac tcacggcgca atttcaacca ccgcgccggc
     3301 actggtaccc tgtacttctg ggaaaaagga ttgccatgca tgagccggta acacacggaa
     3361 ctctcccgcc tgcaggggga tgagagtgta tgtcaggata tacgtcccag cagggagata
     3421 atcagatgtg aacagaatac cgtcatcacg gatttgcggc gaattgaaga gccaccaacc
     3481 ccagccttct gagaatgggt ttttatcctc gaattgaggt tccacttgca cctcggtgga
     3541 atcgattccc tgttggctgg ttttgagggc gcggttcaaa atttcggtgc cggcggggat
     3601 gaagtcttcc accatcacat agtatgcatc gtgaggcagc gtaagtgtga gctgggcagt
     3661 aaccggttga tcggagtcga gctgaaaggt agaaagaggg gtacagccac gcgccgtctg
     3721 tgatcctggg cttcgcgctg cttgacgcgt agcggattcg cagtaaacac gttcgatctg
     3781 catcccggca tccagaggct gcacatcttc caccgggcgg ttaactttta gggtggcgtt
     3841 gtaataaagg cggccgagtc cgtcgtcgcg ggtgatggtc aataggttag gcgaagtggc
     3901 agatagtaaa tcaatcggca caagtgactt taccggtgtg agcacctgat tgccagcgac
     3961 gtcaccattg gcgagaggtc caccattaaa cgtggcattg aatgtgaaat ctgcattgag
     4021 gtcgccgaaa ccgaccatcg cttcgttgag cgcgatcatt gcccaggcgt tatcgtgacc
     4081 tgagttccac agcccgtttg tattgcgatg cgcagcgaga tatcgcaccg cgttgaacaa
     4141 tacctgattc gccgcgtcaa gctgtgcgag cgcgaaggac acaaccgaag ttgtgtaaat
     4201 ggccgatcct ttggttcgga tgttttcacc cgg
//
LOCUS       DASTJP010000560         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_673852, whole genome shotgun sequence.
ACCESSION   DASTJP010000560 DASTJP010000000
VERSION     DASTJP010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673852"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFQ23_16425"
     CDS             complement(<1..390)
                     /locus_tag="VFQ23_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908239.1"
                     /translation="MNIEDAQKIIIQWVKDGAKTEYSNYGYDVYLPNVIRSLLAKEGI
                     DAEREGQVKKHELSPYFYAAAWELCRRGIIRPGVKIMGAQATDDGSSGNGYSITPFGK
                     QWIDEGNKDAFIPTEPGRFAEMLAPYKK"
     gene            complement(684..1625)
                     /gene="lipA"
                     /locus_tag="VFQ23_16430"
     CDS             complement(684..1625)
                     /gene="lipA"
                     /locus_tag="VFQ23_16430"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET9908240.1"
                     /translation="MPVITTRDRIIPETDIRPLRRPEWIKVRAPSGENYERVLNLMRS
                     KSLHTVCEEAMCPNLGECWGSGTATFLMLGDVCTRTCAFCDIKHGKPALMDWGEAERV
                     AQAVKSMDLKHAVITSVNRDERRDGGAPIFAMVIRRIREILPGCSIEVLIPDFKGSLE
                     ALKIVMDARPEILNHNVETVPRLFKIVQPQDNYDWAAATLSNAKKLDPEVLTKSGIMV
                     GLGEEMDEVKAVMRDQRSWGVDILTIGQYLQPSKKHMPISRYYTMEEFAELRDYGKEI
                     GFKWVESNPLVRSSYHAAEQVRALSVVHRKLYGYVNE"
     gene            complement(1720..>2377)
                     /locus_tag="VFQ23_16435"
     CDS             complement(1720..>2377)
                     /locus_tag="VFQ23_16435"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET9908241.1"
                     /translation="NIRTESFDMSFIEEFLLLLVDPNIAFLLIAIGVQAVLIEISSPG
                     GWVAGFIGAVCLTLATYGLGVLPVNWFGIIFLLMAFVLFILDIKAPTHGALTVAGVAS
                     FIVGALVLFNSPGTPDFQRVSVPLVIAVGISIGLLFFGILVFALRALRVPVSAGVESF
                     VGKTGTARTAVDRSGGQVQLQSELWSAELVDESEAISKGDTVEVVEIKGLRLKVKKKS
                     "
BASE COUNT          589 a          676 c          538 g          574 t
ORIGIN      
        1 cttcttatag ggagcaagca tttcagcaaa gcgacccggc tcagtaggga taaaggcatc
       61 cttattacct tcatcaatcc attgttttcc aaatggcgta attgaataac cattcccgct
      121 acttccatca tctgtggctt gtgcgcccat tatttttacg ccaggtcgga taattcccct
      181 gcggcaaagc tcccatgcgg ctgcgtaaaa atatggagac aactcgtgtt tcttgacttg
      241 tccctcccgt tcggcatcga taccttcttt agccaaaagt gacctgatta cattcggaag
      301 ataaacatca tacccataat ttgagtattc tgttttagcg ccatctttta cccactgaat
      361 aattattttc tgtgcatctt caatgttcat aatctttcct tatgaagcgc ctaactagtg
      421 attcgacgga ctccgtataa cagcttatgg gcggactttc aaccgcctaa ttccctaaat
      481 cgtttgggac tttatgcggt gttaggttaa aaaaacatta aaattcagaa aagccaaaca
      541 gaaacaagag cacaaccttc cgaagctacc gcgttcctgg acgattatac gcccgaagaa
      601 cgaccagatt gctcctgtgt aactaaaaac tcccgccgac cttttgagtc aagcgggagt
      661 tactactcac gactttctac tctctattca ttcacatatc catacaactt ccggtgcacc
      721 acactcaagg cacgcacctg ttccgcagca tgatacgaag acctcaccag cgggtttgat
      781 tccacccatt tgaagccaat ttccttgccg taatcgcgca gttcagcgaa ctcttccatc
      841 gtgtaatacc gtgagatggg catgtgcttc ttgctcggct gtaaatattg accgattgta
      901 agaatgtcca cgccccaact gcgctggtcg cgcatcacag ctttgacttc gtccatctct
      961 tcaccaagcc ccaccatgat gccggacttt gtgagtactt caggatcaag ttttttcgcg
     1021 ttggataaag tcgctgccgc ccaatcgtag ttatcctgcg gctggacgat cttaaatagt
     1081 cgcggcacgg tctccacatt gtgattcaaa atttcagggc gggcgtccat cacgatcttc
     1141 agcgcttcca gcgagccttt gaaatcgggg atcaacactt cgatggaaca gccaggtaat
     1201 atctcgcgga tgcgtcgtat gaccatggcg aagatcggcg cgccgccatc gcggcgttca
     1261 tcgcggttca cagacgtgat caccgcatgt ttcaaatcca tggatttcac ggcttgcgca
     1321 acgcgttcgg cttcacccca atccatcaat gccggtttgc cgtgtttaat atcgcagaat
     1381 gcacaggtac gggtgcacac gtcaccgagc atcagaaagg tcgcggtgcc gctgccccag
     1441 cattcgccca ggttggggca catggcttct tcgcaaacgg tatgcaatga tttcgaacgc
     1501 atcaggttca acacgcgttc gtagttctct cccgaaggcg cgcgcacttt gatccactcc
     1561 ggtcggcgca gcgggcggat atctgtttca ggaataattc ggtctctcgt agtgattaca
     1621 ggcattcggt cctttgaaaa atgtggtctc gatacggcgg cgctggtcga gtaggacgtt
     1681 agtccgtatc gagacccgcc gcctactcga ccagcacaat taactctttt tcttaacttt
     1741 caatctcaat cctttgatct cgacgacttc cactgtatct cccttactga tggcttccga
     1801 ctcgtcaact aattctgcgc tccacaactc ggattgcagt tggacctgac cgccgcttcg
     1861 atccaccgca gttcgggctg ttcccgtctt cccgacgaag gattcaactc cggcactgac
     1921 cggcacacgc agcgcgcgta aagcgaacac gaggatccca aagaagagca gaccgatcga
     1981 aatcccaacc gcgatgacca gtggaacgga cacacgctga aaatcaggcg tgcccggcga
     2041 attgaacaac accaacgcac cgacgataaa cgaagcgact ccggccacgg tcagcgcgcc
     2101 gtgggtgggg gctttgatat ccaggataaa taacacaaac gccatgagca gaaagataat
     2161 gccaaaccag ttgacaggca gcacacccag gccatacgtg gcaagcgtga gacataccgc
     2221 gccgataaag cccgctaccc atccacccgg gctcgaaatc tcaatcagca ccgcctgtac
     2281 cccgatcgca atcagcagga aggcgatgtt gggatcgacc agcaggagca ggaattcttc
     2341 gatgaaactc atatcgaacg attcggtgcg gatattc
//
LOCUS       DASTJP010000561         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_466295, whole genome shotgun sequence.
ACCESSION   DASTJP010000561 DASTJP010000000
VERSION     DASTJP010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466295"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            564..890
                     /locus_tag="VFQ23_16440"
                     /pseudo
     CDS             564..890
                     /locus_tag="VFQ23_16440"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
     gene            complement(925..>2689)
                     /locus_tag="VFQ23_16445"
     CDS             complement(925..>2689)
                     /locus_tag="VFQ23_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9908242.1"
                     /translation="DTSVQLFLQRAVRAHVRFDATTADFPAIIRICRLVEGMPLAIEL
                     AAAWVQTLSCGEITREIERGLDILSLPARDLPARHRSMRAVFDHSWKLLTEEEQGVLL
                     RLSVFKGGFRREAAEQVAEATLFVLSMLVTKSLVRRSGAERYDLHELIRQFAAERFTE
                     HPEVQTANQERHSRYYLTFFSQADGRLRSAAQRETLAELILEMDNFRAAWDWAISHHN
                     IARTCQASATLVYVYELRGWLAEGETVFRDAAKAIQSHPVEIENDDEASTAMNAMRAH
                     SAFFSFRLGRSAEAYTELRSSATSLQSCKDQVAAVQSLWYLGIVCWELGKFAEANESL
                     YASLEKARACGERWFEAAAGEFIGIVMHEQGEYEQARQHFIEALAIDRALGDPMITAH
                     VLAYMSQTSQALGETAEAENFLRESLALAQEIGYFSGMGHALDGLGRLTQMTNPTEAR
                     ALFAASYGVYKEFGDLRNLARVLSHQGYNALALGDAASAHDSFIAVLRHAREGGFIPL
                     ALDALVGIASLQAKRGECEQALELLLTILNHPASIQETKDRAARLRTVSEAQLTPLQI
                     EAVQTHAGEKTLVSVVEDLLK"
BASE COUNT          637 a          710 c          697 g          645 t
ORIGIN      
        1 agacctgcag cgctttctga atttcacgta atttgttttg ctctcagggc tggtggacca
       61 tctgaagaag tgtcgatgaa gatatgtaaa tcttttcata ccatcggaaa gcgttttgcg
      121 aaatccttag atgatagata cccctaacca ggttaagaaa aacaacttga cgaatcaatc
      181 atcaagttgt ctagactggc ggtcccgacg ggattagggt tcgcgacgcg taactacttg
      241 ggcacttagt gatttagtga tattttcaat tatagcagta tagcgtagat cgatcaaaca
      301 ttagtgatcc taacctagct gatatgcact ccagcattat ggttcatttg ataagcccta
      361 ggccttcaat tgatggctgc gatgaatccc tgtcgatgtg gcttttatgg taatgctcaa
      421 acgacctgca cctgtgctca ggcaatggcg acgaaatatc aaaagagaat ctccggtccg
      481 gtgctggatc gcattgacat tcacactgag gtgctacgcg tcgaatgcga gaagctaagc
      541 aatgatcgaa tggggagtcg agtgagtcgt ttcgtgctcg tgtacaagcg gcacgtgatt
      601 ttcaacgtaa acgctttgcc acagcaaaac aaaatcagaa gagtttgcac tccgtgattt
      661 gtaatgtaga tatgcgtgtc ggggagattc ggcaattttg tcggttgcag gatgaaggtc
      721 agagcctgat gtgcgctgcg atgagccagc tgaatttatc ggcgcgggcg tatcatcgaa
      781 tcctgaagtt agcccgcacg attgcaggct tagcagggcg cgaggagatc caatccgtgc
      841 acgtggcgga ggcgctgcaa taccgcccta aatcgatgca aaacagttaa tgatgggtat
      901 taaacaaagt gcaacacatt gccgttactt gagtaagtct tccacaactg acacaagagt
      961 cttttctcca gcgtgagttt ggacagcctc gatttgcaat ggagtcaatt gcgcttccga
     1021 tacggtgcgc agacgggcgg cacggtcttt ggtctcttga atgctggcgg gatgattcag
     1081 gatagtcaat agcaattcaa gcgcctgctc gcactctccc cgtttcgctt gcaggcttgc
     1141 gatgccaaca agcgcatcaa gagcaagagg tataaaaccg ccttcgcgtg catggcgcaa
     1201 tactgcaatg aaggagtcgt gtgcgctggc agcatcgccc agagcaagcg cgttgtatcc
     1261 ttggtgactg aggacccgcg ccaagtttcg gagatcgcca aattccttat acacaccata
     1321 gcttgccgca aacaaggcgc gtgcttccgt gggattggtc atttgggtaa ggcgtcccaa
     1381 cccatcgagt gcgtgtccca taccggaaaa atacccaatc tcctgtgcaa gagctagact
     1441 ctcgcgcagg aaattttcag cttctgcggt ctcacccaat gcctgactcg tttgactcat
     1501 atatgcgagt acatgagcag tgatcatcgg atcccctagt gcccgatcaa ttgccagcgc
     1561 ctcgataaaa tgctgtcgtg cctgctcgta ctcaccctgt tcgtgcataa cgatcccgat
     1621 gaattcgccc gccgccgcct caaaccaacg ctcgccgcac gctcgggctt tttccagact
     1681 ggcgtacaaa ctttcattcg cctctgcaaa tttgccaagt tcccagcaca cgatgcccag
     1741 ataccacagc gattggactg ctgcaacctg gtctttgcac gattgcaggc tggttgcact
     1801 tgaccgcagc tcagtatatg cctctgcgct ccttccaagg cgaaaactga agaaggctga
     1861 gtgtgcacgc attgcgttca tagcggtcga cgcctcatca tcattttcga tctccacagg
     1921 gtgtgattgg attgccttgg cagcgtcgcg aaaaactgtc tcaccttctg cgagccagcc
     1981 ccgcaattca tacacatata cgagcgtcgc cgacgcttga cacgtgcgag cgatgttgtg
     2041 atgcgatatc gcccaatccc acgcggctcg gaagttatcc atctcaagga tcaactcggc
     2101 gagagtctcg cgctgtgcgg cgcttcgcag acgtccatcc gcctgactga aaaatgtcag
     2161 gtaatatctg ctatgacgct cttggtttgc agtttgtact tccggatgtt cagtgaagcg
     2221 ttcagcagca aactggcgga tgagttcatg cagatcatag cgttctgcgc cgcttcttcg
     2281 gaccaatgat tttgtcacca acatggaaag tacaaagagt gtcgcttcag caacctgctc
     2341 tgccgcctcg cgacggaagc cgcccttgaa tacggaaagc cgaagcagaa ctccctgctc
     2401 ttcttctgtg agtagtttcc acgagtggtc aaagacggcg cgcatggagc ggtgacgagc
     2461 aggaagatcg cgcgccggta ggctcaggat atccagtccc cgctcgatct cccgcgtgat
     2521 ctcgccacag gacagagtct gcacccaggc cgcggccagt tcaatcgcca gcggcattcc
     2581 ttccaccagt cggcagatgc ggatgatggc aggaaaatcc gctgtcgtcg cgtcgaatct
     2641 cacatgtgcc cgtacggcgc gttgcaggaa caactgtacg gatgtgtcc
//
LOCUS       DASTJP010000562         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_360675, whole genome shotgun sequence.
ACCESSION   DASTJP010000562 DASTJP010000000
VERSION     DASTJP010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360675"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFQ23_16450"
     CDS             <1..445
                     /locus_tag="VFQ23_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908243.1"
                     /translation="IGETGEVLGTGTPENTPLPPSPTVVPAPNDNDSAQAPAVNVTFS
                     ANGTRVLQYSSDLSAPTGDAEDWVQFSPFTQTVLLELTCRGNGNLTVSLLQYNLSVPD
                     WGTLPCGATQAVTVNVNTVYLLRLQAAASDSLSYTQFTVTVRSVP"
     gene            551..1168
                     /locus_tag="VFQ23_16455"
     CDS             551..1168
                     /locus_tag="VFQ23_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET9908244.1"
                     /translation="MNADRIPDIIIGRLPVYLRALQRLAEKGIQTTSSQELGEIIGIS
                     AAQIRKDISQFGEFGKQGTGYSIPFLIERLRDILQVDRIWDMAVVGMGDIGHALARYN
                     GFSNRGFRVVMVFDNDPKKIGDKVGEFEVFSTDEMIERIKQNKIKVAVLTVPAPVAQE
                     ITDQLVKAGVKAILNYAPISLNVPDDVHVQYIDPVTHLQRMTYYL"
     gene            1557..2735
                     /locus_tag="VFQ23_16460"
     CDS             1557..2735
                     /locus_tag="VFQ23_16460"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908245.1"
                     /translation="MGKITVLPKPAGPFPVGVTHLNFIDESRVDVSPQANGKNRDVPI
                     MIWYPVDEPGERIPSKLLKIRDLVSLKHYPLYKLIPNSVCDIRTNSYQDVPLSTQNST
                     FPVLIFNHGFSSYMEQNTILMEHLTSFGYIVVSVGHPYDGVASYPDGRSIPVDLETIK
                     ELTKQSRETSKTFSKNIKLLAGNDLSLEDMRTFTGNYLHASKAMNDKVEIWVEDVRFI
                     INILEKMDSGFIPSQFAHKLSLEKGIGAFGQSYGGSASVLACCLDRRLRCAINMDGAM
                     YGGMNHKYKYTRPTMYMDSDMLPGRSKYFFYINEDDTYHIMIKGSKHLDYSDFTYISK
                     IWLLKLMQLFGKIDGRLMIKITNEYVLAFFDKYIRQLDVPLLSQSPYSEVVFEKRIHE
                     "
BASE COUNT          778 a          710 c          703 g          710 t
ORIGIN      
        1 catcggggag accggcgaag tgctcggcac aggaacgccg gagaacacgc cgctgcctcc
       61 ttcgccgaca gttgtcccgg ctccgaacga caatgactcc gcccaggccc cggctgtcaa
      121 tgtgaccttc tccgccaatg ggacgcgcgt cctgcaatat tccagtgatc tctccgcccc
      181 aaccggcgac gccgaggatt gggtgcagtt ctcgccgttc acacagaccg tcctgctcga
      241 actgacctgc cgcggcaatg gcaatctgac cgtgagcctg ttgcagtaca acctgtctgt
      301 gcccgattgg ggcacgctcc cctgtggcgc gacgcaggca gttaccgtta acgttaacac
      361 ggtctatttg ctccgcctgc aagccgccgc gagcgacagc ctgagctata cccaattcac
      421 ggtgacggtt cgttcggtgc cataattctg accccagcgg ggcatttgtg taagtttgta
      481 caaaaaaatc cttttatggt agagttaata tattaattca cattaaccta tttcaaagag
      541 ggctgactga atgaacgcgg acagaattcc agacatcatc atcggcaggt tgcccgttta
      601 tttgcgcgcg ctccaacggc tggcggaaaa aggcattcaa accacctctt cacaagaact
      661 gggggagatc atcggcatct ccgccgcgca gatccgcaag gatatttcac agttcggcga
      721 gttcggcaaa caagggaccg ggtactccat cccttttctg atcgaacgct tgcgcgacat
      781 cctgcaagtc gaccgcatct gggatatggc ggtcgtaggc atgggggata tcgggcatgc
      841 gctggcgcgc tacaatggct tcagcaatcg cggcttccgt gtggtcatgg tcttcgataa
      901 cgaccccaaa aagatcgggg acaaggtcgg ggaattcgag gtcttcagta cggacgagat
      961 gatcgagcgc atcaagcaga acaagatcaa ggtcgccgtg cttaccgtcc ctgcgccggt
     1021 ggcccaagag atcactgacc aactcgtcaa ggcgggggtc aaagccattc tcaattacgc
     1081 ccccatcagc ctcaatgtgc ccgacgatgt gcacgtccaa tacattgacc cggtcacgca
     1141 tttgcagagg atgacgtatt atttgtagga caagctaatc cttgaccgta tttgggcgat
     1201 tcccatgaag gggactacat ggccagaacg agccgcccat aagcctttat acggaattaa
     1261 ccgtctaaac actagctggg cactgattga aaatgactat cacagacatt attacagcag
     1321 tcaaagaaaa tcataaatcg aaaaaccgtg tctaacatgg cgtcccggaa gagtaccggg
     1381 ataatgcacc caacttgggg tacgcgccgc gttatttggc atttttgcgg cctcgggttt
     1441 tttctgcttc caggcagagt ccacgcccac cccagcgcca gttgcgcaaa tcgttaggca
     1501 caccagagcg aacaaacaga tggtggtccg tctgctttgc aggatgatca gcaagcatgg
     1561 gaaaaatcac tgtattacca aaaccagccg gtccatttcc ggttggggtt actcatctca
     1621 atttcattga tgaaagtcgt gtcgatgtct ctccacaggc taatgggaaa aacagagatg
     1681 ttcccatcat gatatggtat ccagttgatg aaccaggtga aagaattccc tcaaaattgc
     1741 tgaaaatccg tgatctcgtg agtttaaaac attatcccct ttataaatta attcccaata
     1801 gcgtttgtga tatcaggaca aatagttatc aagacgttcc cctatcaacc caaaactcca
     1861 cattcccggt tctgattttt aatcatggat tttcgtcata tatggaacag aacacgattt
     1921 tgatggagca cttgacgagt tttggctata ttgttgtttc tgtaggacat ccctatgatg
     1981 gcgtagcatc ttatccggat gggcggagca tacccgtaga tttggaaacg atcaaggaat
     2041 tgacaaaaca atccagagag acttcgaaaa cattcagcaa aaacatcaag ctattggcgg
     2101 gaaatgattt atcgctggaa gatatgagaa catttaccgg gaattatcta catgcaagca
     2161 aagcgatgaa tgataaagtc gagatatggg ttgaggatgt tcgatttatc ataaacatcc
     2221 tggagaaaat ggatagtggg ttcattccat cccaatttgc acataagctc tctctcgaaa
     2281 agggtattgg tgcattcggt caatcctatg gagggtccgc gtccgtttta gcttgctgtc
     2341 tcgatcgccg cctcagatgc gcaatcaata tggatggtgc catgtatgga ggaatgaatc
     2401 ataaatacaa gtatacgcgg ccaaccatgt atatggatag cgatatgttg ccgggcagaa
     2461 gcaaatattt cttttatatc aatgaagatg acacatacca tatcatgatc aaaggttcca
     2521 agcatttgga ttattctgac tttacataca tctctaaaat ttggctgctg aaattgatgc
     2581 aattatttgg caaaattgat ggaaggctga tgatcaagat cacaaatgag tatgttctag
     2641 cattctttga taaatacatt aggcagctcg atgtacctct cttaagtcag agtccatata
     2701 gtgaggttgt ttttgaaaag cgaattcacg aatgatgggt taatcgacca cgagcagtcc
     2761 aaaatggatc aaagataggc aatgttgaaa ggcgcctaac ataacgtgca actgacggtg
     2821 ggtacgcgcc gcgtttttgg agcatttctc cggcttgcgt agcctcccgt ttagatgacg
     2881 agtccaccct cccaccccaa g
//
LOCUS       DASTJP010000563         4823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1289577, whole genome shotgun sequence.
ACCESSION   DASTJP010000563 DASTJP010000000
VERSION     DASTJP010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289577"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..1330
                     /locus_tag="VFQ23_16465"
     CDS             368..1330
                     /locus_tag="VFQ23_16465"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET9908246.1"
                     /translation="MNNYRTSLPQLDNKLYLTDGGIETTLIFHEGLELPDFAAFHLLQ
                     SENGEKALQKYFRTYAEIAKRFDTGLILESATWRASKDWGARLGYTPEALVDVNRRAI
                     QMLEDLRNEFESDQTPVVLSGCIGPRGDGYVPGNVMTAQEAEDYHWEQIKTFANSGAD
                     MVTAITMNYVEEAIGITRAAKRANIPVAISFTVETDGNLPTGQSLKTAIEQVDEATSG
                     YPAYYMINCAHPTHFENVVAEGGAWLERIRGVRANASHRSHAELNEAPDLDDGNPVEL
                     GAQYASLTKHLSRLNVMGGCCGTDHRHLEQIAEACVSLFQPSVE"
     gene            1415..1909
                     /locus_tag="VFQ23_16470"
     CDS             1415..1909
                     /locus_tag="VFQ23_16470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908247.1"
                     /translation="MKVKIRHVQRQDLSAIHGILVSPHVIQGTMRLPYHSLEYVEKRI
                     EPSDGTIKLVACVENEVIGYGELATHSDAPRHRHVADINILAVNSNWLGKGIGGAILR
                     SMLDLADNWMQVTRMSLIVWSSNENAISLYKKHGFELEGTLRQFVFKEGRYEDALLMA
                     RIKP"
     gene            complement(2123..4690)
                     /locus_tag="VFQ23_16475"
     CDS             complement(2123..4690)
                     /locus_tag="VFQ23_16475"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9908248.1"
                     /translation="MAEVSFGEWLKRQRGANGWTQKQLAQQMNCSVSALRKMEAEERR
                     PSVQVMDRMAEIFHIPLNKRKSFLQFARGDWYAFSIPDGEIEDVSWHIPSKASQSNLP
                     ASFTSFVGREKDQVEILELIKKRRLVTLVGAGGIGKTRLALQLAQNLLNDHPGGVWFV
                     ALESLSDPALIPSTVAAIFEIRDSPTGRPLVERLTESLRPKTALLILDNCEHLLEGCI
                     ELITTLLTHCPHLKILTTSRASLNMEGEATYYLSSLSLPNEDEIASEKAGTNECVRLF
                     SDRAELALTSFQLTSDTTQSCTDICRLLDGIPLAIELAAARVDILQAHEILQQLQHCF
                     DLLFSDHRGAIARHQTMRASMDWSWELLNETEQQFLQQLSVFAGGWTLESAQAVCRGD
                     VIGLTSALVKKSLIMVIQEAGRTTRYRFHEIVRQYTREKWVKAGDEAEIRTGHLRYFL
                     QFAERAESALKSSAQMEWYLHLQKERNNLRAALEWADQTDTEAGLFISGRLHRFWENF
                     DMREGQHWLDTFLEKPESKNYPQARASALYTKTWMLHWSQKPASALSAAKECLDLYKS
                     SGNIPGEIDGLLALVLVAPAAGNIDGIELYHRALTLSKSLSDPWKQAQVLFQSRFLTL
                     DDYHHTSYLAKSVTYFKKAGDLEWSIQPMLWLANEEMTKGDLPSAQQWMSQAMQAGQA
                     LHHKGLRSLLLLSMGSMELLSGEYEKARANLEEAIQLYGDLGNRLAILWARVRLGYVA
                     VRQGELTKARDLLTETAQDFRESQGPSGVIFALEGMANLHVASEKPEHAARLIGWADF
                     KRKETNNKRQILEQTDVDKFIEACLAKMGEVAFSDAYDEGQKMSLDEAVAYALQETD"
BASE COUNT         1244 a         1192 c         1137 g         1250 t
ORIGIN      
        1 ccatcgatgc gatcctcatc agcgaataac agacggagca tatgagagtg tgacgtgggg
       61 cagggtacaa tgaaatatat aaccctgccc cacgaatcat gttcccgatt tatcgggata
      121 tataaaaaag cctgattaga aactagtgac ttgacactcc gtttcaaaag gaatccgttg
      181 gtcgagtaag cggtgttctc ccgctgtatc gagaccaaca gcaacttgca aatgaagcat
      241 ttcagaaaat ttgtggtctc gacaccgtgc ctgcgttcgg tgcaggtgta cgtcacgcca
      301 cgctcatgcg ggactactcg accaccggcg acttttagaa accaaattat attaaaggaa
      361 atgaaccatg aacaattatc gaacttcact acctcagctt gataataagc tttatcttac
      421 agatggtgga attgaaacca ccttgatctt tcacgaaggt cttgaactcc cagactttgc
      481 cgcctttcat ttattgcaat cagaaaatgg tgagaaggca ctccagaaat acttccgtac
      541 ctatgccgag atcgcaaaac ggtttgatac aggtcttatc ctggaaagcg ccacctggcg
      601 tgccagcaaa gactggggtg cacgactcgg atacacacca gaggcattag tcgatgtcaa
      661 ccgccgggca atccagatgc ttgaagatct ccgaaacgaa tttgaaagcg accaaactcc
      721 tgtcgtgctc agcggatgta tcggtccgcg cggcgatgga tatgttcctg gcaacgtgat
      781 gaccgcccag gaagcagaag actatcattg ggaacagatc aaaacctttg cgaactctgg
      841 tgcagacatg gtcactgcca ttacgatgaa ctacgtggag gaagcgattg ggattacacg
      901 tgccgcaaag cgcgcaaata ttcctgttgc catttccttc acggtagaga cggatggtaa
      961 tctacccaca ggtcaatctc tgaagacggc catcgaacag gtggacgaag ccacatcagg
     1021 atatccggcg tattacatga tcaactgtgc ccatccaact catttcgaaa atgttgtcgc
     1081 ggaaggtggc gcgtggctgg aacggattcg cggtgtgcgc gcaaacgcgt cccatcgcag
     1141 ccatgcagaa ttgaacgagg ctcccgatct cgatgatggt aatcccgtcg aacttggcgc
     1201 gcaatatgca agcctcacaa agcatttatc acgcctgaat gtgatgggtg gctgctgtgg
     1261 aaccgatcat cgccatttgg aacagattgc ggaagcctgc gtgtcactgt ttcaaccttc
     1321 ggttgagtaa gatcaaacag gactctttga aatctacaag acttcaagat ccttggcttt
     1381 cacacccgat ttgtttcata agggctttaa taatatgaaa gtgaaaattc gtcacgttca
     1441 aagacaagat ctgtctgcca tccatggcat cttggtcagc cctcatgtca ttcaagggac
     1501 gatgcgcctc ccatatcatt cgcttgaata tgtcgaaaaa cgtatcgaac cgagcgacgg
     1561 cacgatcaag ttggtggcct gtgtagaaaa tgaagtgatt ggctacgggg agcttgcaac
     1621 gcattccgat gcgccccgtc accgtcacgt agccgacatc aatatccttg ctgtgaattc
     1681 taactggctg gggaagggga ttggtggagc catactgcgg tccatgcttg accttgcgga
     1741 taattggatg caagtcactc gtatgagcct gatagtttgg agttcaaatg aaaacgccat
     1801 cagcttgtat aaaaaacacg gttttgaact tgaagggact ttgaggcaat tcgttttcaa
     1861 agaaggcagg tatgaagatg cgcttttgat ggccaggatt aagccttgaa tcttaatgag
     1921 atgttcaatg cgcctgcctc caaaaaattt atcatgcctg aatgagataa gtggctcctg
     1981 cggaatagcc catctcaatt tggtacggat cgcggggcca gcatgtcgct gtttggagtt
     2041 gcggcttatt taatggataa tgtgtgcaat ctctaacttg caaattgcgg atgagaaaac
     2101 ttctcagccg cagtttgttt tgctaatcag tctcttgcaa tgcatacgca actgcttcat
     2161 cgagcgacat cttctgacct tcatcatacg cgtccgaaaa cgctacctca cccatctttg
     2221 caagacaagc ctcgatgaac ttatccacat ctgtctgctc gaggatctgc cgcttattat
     2281 tggtttcttt acgcttgaaa tccgcccagc cgattagccg cgcggcatgc tcaggttttt
     2341 ctgatgcaac atgcaagttt gccattcctt ccagagcgaa tatgacaccg gatggtccct
     2401 ggctttcccg aaagtcttgt gctgtctcag tgagaaggtc gcgtgccttc gtcaattcac
     2461 cttgacgcac agccacatac cccaaccgaa cgcgcgccca gaggattgcc aggcgatttc
     2521 ccaaatctcc ataaagttgt atcgcttctt ctaaattagc acgtgctttt tcatactccc
     2581 cactgagtaa ttccatgctg cccatggaaa ggagcaacaa acttcggagc cctttgtggt
     2641 gtagtgcctg cccggcttgc attgcctgac tcatccattg ttgagcggat ggaagatcac
     2701 cttttgtcat ttcctcgttt gccagccaca gcatgggttg gatcgaccat tccaggtcac
     2761 cggctttctt gaagtaagtc acagacttgg ccaggtaaga agtatgatga taatcatcta
     2821 gagtcagaaa gcgtgattga aacaacactt gagcctgttt ccacggatcg ctcagcgatt
     2881 tggacaaagt cagtgcccgg tgatagagct cgataccatc gatatttcca gctgcaggtg
     2941 ctaccaggac caatgcaagc agtccatcta tttctccggg tatattaccg ctggatttat
     3001 ataaatccag gcattctttt gctgccgata aagccgacgc aggtttttgc gaccaatgca
     3061 acatccaggt ctttgtatat aatgcgcttg cgcgggcctg gggataattt tttgactctg
     3121 gcttttcaag gaaggtatcc agccaatgct gaccttcacg catatcgaaa ttttcccaga
     3181 agcggtgaag cctgcctgat ataaatagac ccgcctccgt gtccgtttga tccgcccatt
     3241 ccaaagcagc gcgcagatta ttacgttcct tttgtaggtg aagataccat tccatttgcg
     3301 cggaactctt gagcgcagac tctgcccttt cggcaaattg cagaaaatat cttaaatgcc
     3361 ctgttcggat ttcagcctcg tcaccagcct tgacccattt ttcccgtgtg tattgacgca
     3421 caatttcatg aaagcgataa cgtgtcgtgc gacccgcttc ttgaataacc ataataagcg
     3481 acttcttcac gagtgcactg gtcaaaccaa taacatcacc tctacatacg gcttgagccg
     3541 attcaagtgt ccagccgccc gcaaatacag aaagttgctg caagaattgt tgctcagttt
     3601 cgtttaataa ttcccaactc caatccatgg aggcgcgcat ggtttgatgg cgcgcgattg
     3661 ctccacgatg atcactgaag agcaggtcga aacagtgctg cagttgttgt aatatttcgt
     3721 gtgcctgcaa aatatctacg cgggccgccg cgagttcgat agccaatggt atcccatcca
     3781 gtaagcggca aatatcggta cagctctgtg tggtatccga cgtcaactga aaagatgtga
     3841 gagccagctc cgcgcggtct gaaaacaacc gaacacactc atttgtgcct gctttttcgc
     3901 tggcaatttc atcttcattc ggcagggaaa gtgacgataa ataatatgtt gcttcacctt
     3961 ccatatttag gctcgcgcgg ctggtggtaa ggatttttag atgcggacaa tgggttagca
     4021 aagtcgttat cagctctata cagccttcca gcaaatgttc gcaattatct agaattaaaa
     4081 gggcggtttt gggacgcagg gactcggtca gtctttccac gagagggcgg ccagtaggac
     4141 tatcccgaat ttcaaaaatc gcagcaacag tggagggaat caatgccggg tcagaaagag
     4201 attcaagtgc aacaaaccac acaccgccgg gatgatcatt caagaggttt tgagccaatt
     4261 gtagagccag gcgagtcttg ccaatgccgc ctgcgcccac cagagtgacg aggcggcgtt
     4321 tcttaatgag ctctagaatt tcaacttgat ctttttcccg acctacgaaa gatgtaaacg
     4381 acgctggcaa attggattga gatgctttgg atggtatgtg ccaggacaca tcttcgatct
     4441 ccccgtcggg gattgaaaat gcataccaat caccgcgtgc aaattgaaga aatgattttc
     4501 gcttgttcag agggatatgg aaaatctctg ccattcgatc catgacctga actgatggcc
     4561 ggcgttcttc tgcttccatt ttgcgcaatg cactgaccga gcaattcatt tgctgagcga
     4621 gttgcttttg tgtccaccca ttcgcgcccc gttggcgttt gagccattct ccgaaagata
     4681 cctcagccaa agtctcctcc acaatttgtg tcactgttgt gtcactgagc gacacctgca
     4741 tgtttgcgat tccccctata gtttaaccgt tgattcaaag tatatcaatc tcatcacgca
     4801 acttattgaa tctgggaata cat
//
LOCUS       DASTJP010000564         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_919402, whole genome shotgun sequence.
ACCESSION   DASTJP010000564 DASTJP010000000
VERSION     DASTJP010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919402"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFQ23_16480"
     CDS             <1..287
                     /locus_tag="VFQ23_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908249.1"
                     /translation="ELEASDWQIMNQTKLPVVCFVDGQNNMEGRSGAFIEAVAREVVS
                     SGEAWISVTRVKDDVPVLRACITSYRTTPEDVLTLVHAIDAARRKIYGRA"
     gene            645..1307
                     /locus_tag="VFQ23_16485"
     CDS             645..1307
                     /locus_tag="VFQ23_16485"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HET9908250.1"
                     /translation="MKKVTQFGETVEGYNIPVLNEREIRASAGILYLVMFWSWILVIF
                     NDNYLLLKYANTIFLADFIVRIFISPKYSPLLIIGRLIVSNQTPEYVGAAPKKFAWTI
                     GLVLAAIIFLLLVVMNSDSLINFFICQTCLIFLFFESAFGICLGCKSYSMFHKENAQY
                     CPGEICDVKAKQEIQKTSLLQIAVVIGFFAFVITTAILFNDGFGAKPTSLWVIVKSAF
                     SI"
     gene            1405..2394
                     /locus_tag="VFQ23_16490"
     CDS             1405..2394
                     /locus_tag="VFQ23_16490"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9908251.1"
                     /translation="MRAIRFTKYGPPDLLQLIEVETPTPNPDQVLVRVYAAAANPLDW
                     HRMRGAPFLVRIGDGLLKPKNPKLGADIAGRVEAVGSAVTGFQPGDEVFGSVGSGGFA
                     EYVCARARNLAHKPGNVSFEEAAAAPVVGLTALQGLRDIGHIQAGQKVLVNGASGGVG
                     HFAVQIAKAYGAEVTGVCSTRNLDMVRSLGADHVIDYIREDVSRTGQRYDLMYDSIGN
                     RSVFAYRRALKPQGVCVIAGFTGFSRLLEQMTLGKLLSKDGGRKIGGMGMAKVTSEDL
                     LVIKELLETGKVRPVIDRSYPLSETAEAIRYLETGHAKGKVVITVQSITKQDV"
BASE COUNT          669 a          538 c          606 g          610 t
ORIGIN      
        1 gagaactgga agcgtcagat tggcaaatca tgaatcagac taaattgcct gtcgtgtgtt
       61 ttgtggatgg gcaaaataat atggaaggca ggtctggagc cttcatcgag gcagtcgccc
      121 gagaggtggt ttcctcagga gaagcctgga tttctgttac gcgtgtaaaa gacgacgtgc
      181 ctgttcttcg cgcgtgtatt acaagctacc gtacaacacc cgaggatgtg cttacgctgg
      241 tacatgcaat tgatgcagct cggcgtaaga tttatggtcg cgcctgaaaa agcgttctca
      301 gaacgtatcc cagaaattgc gaaaaaatgc ctcttttcct caaaaaccgc cacttttcac
      361 agactcctgc aaggtgagac gttgtttcgt ggaaaatgat cgcctgccat cattcgaagg
      421 tgcacaggac aggtgggatt ctgccgcatt ttcaaacagt ttcctggctt cgagtttata
      481 ctgctcccaa gcattgtcca cgcccgccca cctgccagta acgcaacccg ttcagcgttt
      541 tcgacttcaa gctccaacag caaataagaa ttttggagag gttgactctc aacctctcca
      601 aaattgaaaa gtaaaaaaaa caatgataga gaaaggaatg aaaaatgaaa aaagtaacac
      661 aatttgggga aaccgtggaa ggttacaaca tccctgtttt aaacgagcgg gagataaggg
      721 catcggcagg aatactatat ttggttatgt tctggtcctg gatactggtt atatttaatg
      781 ataattatct cctgctaaag tatgccaaca cgatcttcct tgcagatttc atcgtacgca
      841 tatttattag ccctaaatat tctcctttat taataatcgg gcgtttaatt gtcagcaatc
      901 aaacacctga atatgtgggg gctgccccaa aaaagtttgc ctggacaatc ggtctggttt
      961 tagcagcaat catattcctt ctattggttg taatgaattc agatagcctc attaattttt
     1021 ttatttgtca aacctgcctg atattcttat tctttgaatc tgcgtttgga atttgtttgg
     1081 ggtgtaaatc ttattcaatg ttccataaag aaaatgctca atactgcccg ggcgaaattt
     1141 gtgatgtaaa ggcaaaacaa gagattcaga aaacgtcgtt actgcaaatc gctgttgtta
     1201 ttggtttttt cgcgtttgtt ataacgacag ccatcctgtt caacgatggg tttggcgcaa
     1261 aaccgacttc tctgtgggtt atcgtgaaat ctgctttttc aatataaata ttgtttttca
     1321 caaaaattaa ttgcaaaaag acaggagaaa gtaaattatg aacagcgaca gtctacaaac
     1381 tacagaccat ctcggagaca acatatgaga gccattcgat tcacaaaata cggacccccc
     1441 gatctgcttc agctcattga ggtcgaaacg cccaccccaa acccggatca agttctggtg
     1501 agagtttatg cggcagcggc caatccgctg gactggcatc ggatgcgagg cgcacctttc
     1561 ctggtccgca tcggtgacgg actgctcaag cccaaaaacc cgaaattggg agcagacatc
     1621 gcgggacgtg tcgaagcagt tggcagcgct gtcaccgggt ttcagccggg agatgaggta
     1681 ttcggctcgg tgggatcagg cggcttcgcc gaatatgtat gtgcccgtgc aagaaacctg
     1741 gcgcacaaac cgggcaatgt ctcatttgag gaggccgcgg ccgcgccagt tgtaggcttg
     1801 acggcactgc agggtcttcg cgatataggt catatccagg cggggcaaaa ggttcttgtc
     1861 aacggcgcat ccggcggggt tgggcatttc gctgtacaga tcgccaaagc atacggagcg
     1921 gaagttaccg gcgtgtgcag cacaaggaat ctcgacatgg tgcgctcgct cggcgcagac
     1981 catgtcattg attacatacg ggaggatgtc agtagaactg gacagcgtta tgacttgatg
     2041 tacgactcca tcgggaatcg ttccgtcttc gcgtaccgac gtgcgttgaa acctcagggc
     2101 gtctgtgtca tcgccgggtt tacaggcttt tcgcgtttgc tcgaacagat gaccctgggg
     2161 aaactactat caaaggacgg cggcaggaag atcggtggga tgggaatggc gaaagtaacc
     2221 agcgaagatc tgctcgtgat caaagagttg cttgagacgg gcaaagtcag acccgtgatc
     2281 gacaggtcgt atccattaag tgagacagcg gaagccatac gatatcttga aacaggacac
     2341 gccaaaggaa aagtggtcat cactgtacaa tcaataacaa aacaggatgt gtaaccatga
     2401 aggcaatcat atggacaaaa tac
//
LOCUS       DASTJP010000565         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_864129, whole genome shotgun sequence.
ACCESSION   DASTJP010000565 DASTJP010000000
VERSION     DASTJP010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864129"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFQ23_16495"
     CDS             <1..440
                     /locus_tag="VFQ23_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908252.1"
                     /translation="KIDFQAEQNNMVISEMVPQSESVDNWSTLITVQIFLGMTNTTPE
                     QYQDTLTQSWFAACQDSASYPVADGTENGYNFVLWQLYCPLNPSTQKVEFTYLKAIQG
                     NDSFYLVQVAFRHEPTSDEITEWMNYLRTVQVCDSRIPESACP"
     gene            599..1012
                     /locus_tag="VFQ23_16500"
     CDS             599..1012
                     /locus_tag="VFQ23_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908253.1"
                     /translation="MQHTTTQPRIAAITTQVVVRKSTFMIALAALGSMSILAGIISSV
                     SAIVLSSEGASTGMVYSVLADVACNFVLGALIFASWRAFAQGKMISVWLFGSSLLVDS
                     LYSLVMGYQMHYVFVGLGLLLIWQMRQYKAELELS"
     gene            complement(1066..2388)
                     /gene="ffh"
                     /locus_tag="VFQ23_16505"
     CDS             complement(1066..2388)
                     /gene="ffh"
                     /locus_tag="VFQ23_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET9908254.1"
                     /translation="MFENLTNRLNDVFDQLRRRGKLSEADVDAAMREVRLALLEADVH
                     FSVVKAFIARVRERAVGAEVSRALNPGQQVIKIVNDELIATLGEPAPLNLTGPKPRVI
                     MMVGLQGSGKTTGAGKLARLLRSKGERVMLVAGDPYRPAAVTQLQQLGERVDVPVESD
                     LNLKPPDLVKRSFEKAEKGGFGVMIVDTAGRSQLDAELMNELKSIVAKVPPVETLLVV
                     DSMIGQEALNIAQGFRDNVSITGLMMTKMDGDSRGGAAISIRSVTGVPIKFIGTGEKL
                     DALETFDPARLSSRILGMGDMIGLIEKAEAAFDQQTAQKTAERMMKGQFSLEDWLDQM
                     KQMKKMGPLAQIMDMLPGGLGQAARQVDPKEIERTFKQSEAIINSMTLKERRDPEILN
                     ASRRRRIAAGSGMEVQDVNRLIKQFREAQKMMKMLQKTGGKGLGRLLG"
     gene            2636..2708
                     /locus_tag="VFQ23_16510"
     tRNA            2636..2708
                     /locus_tag="VFQ23_16510"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2669..2671,aa:Phe,seq:gaa)
     gene            2737..2808
                     /locus_tag="VFQ23_16515"
     tRNA            2737..2808
                     /locus_tag="VFQ23_16515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2769..2771,aa:Gly,seq:gcc)
     gene            2825..2896
                     /locus_tag="VFQ23_16520"
     tRNA            2825..2896
                     /locus_tag="VFQ23_16520"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2857..2859,aa:Cys,seq:gca)
     gene            3047..>4272
                     /locus_tag="VFQ23_16525"
     CDS             3047..>4272
                     /locus_tag="VFQ23_16525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9908255.1"
                     /translation="MKILYVEDEIAHVVLTQRTLEDSIQHEFTLLHAETIGDALRILD
                     AEPDIDLILSDLRLPDGTGLDLLKKVRERVAAPAVVLVTGQGDQEVAVTALKAGAADY
                     LVKQSDYLHRLPIVIGNAIAQNRYLRDQAALREAEIKYQSLVEQIPAVVFLDRADESE
                     ATFYMSPRIESLTGFTPQEWYSTENIWFDRIHAADRKRIDDAYEKSHVNGTRFFEEYR
                     FLRRDGRTIWVKEDTNLIRDEMGNPLYWQGILLDITKEKESQTFLKESEERFRRIFHS
                     SPIATCVVTLEDGKFIDANEAFQNLVGIPLENLIGHTSVELGFWREGTDRESFIFHLQ
                     DERALQGVEIQYTNVPNGPKDTLAYYELIELGGQSCILSMFYEITEQKKSQKALQRQL
                     EELSVLHSVATAGTES"
BASE COUNT         1055 a         1042 c         1048 g         1127 t
ORIGIN      
        1 tcaagatcga ttttcaggca gaacaaaata acatggtaat tagtgagatg gtgccgcaga
       61 gtgagtcagt cgacaactgg tctacattga tcacagtcca gatttttcta gggatgacga
      121 ataccacacc tgagcaatat caagacaccc tgacccagtc gtggttcgcg gcctgtcagg
      181 atagcgcgtc ctatcccgtg gcagatggca ctgaaaatgg atataacttt gttctgtggc
      241 aactttactg tccgcttaac ccttccacgc agaaggttga gtttacgtat ttgaaagcta
      301 tacagggaaa cgacagtttt tatctcgtcc aggtcgcttt caggcatgaa ccaacttcgg
      361 atgaaatcac agaatggatg aattatcttc ggacagtcca ggtctgtgat tcgagaatcc
      421 ctgagagtgc atgcccataa tttccagtgc gattcgtgga acaagctgag cctgcaattg
      481 gtagtggatg taaagcaact gcaaacttta agtctgattc tttttacgat gactccgtca
      541 tgtagactga aatcgttgtg aacattgaaa ttgtacaaaa gatcttaaag gagcaaatat
      601 gcaacacaca accacacaac caagaattgc cgcaatcacc actcaagtgg ttgtacgaaa
      661 atctactttt atgattgccc ttgcggcttt gggcagtatg agtattttgg caggtattat
      721 tagttcagtt tcagccattg tcctatcatc cgagggcgca tcgactggca tggtttattc
      781 ggttctggca gatgtcgctt gcaatttcgt tctgggcgcc ttaatctttg ccagctggag
      841 agctttcgct caagggaaaa tgatatccgt ctggctcttt ggaagcagct tgctcgtgga
      901 cagtttatat agcctcgtca tgggatatca aatgcattat gtttttgtgg gtcttgggtt
      961 gttactgatt tggcaaatga gacagtacaa agcagaattg gaactctcgt aactccggta
     1021 tgcaacttca caaaaagtcg gtcacgcaag gcgtgaccga cttttttatc ccaacaatct
     1081 tcccaatccc tttcccccgg ttttctgcaa cattttcatc attttctggg cttcacggaa
     1141 ctgcttgatc agccggttta catcttgcac ttccatgcca gagccagccg cgatgcgccg
     1201 tctacgggat gcgttcagga tttctggatc gcggcgttct ttcaacgtca tcgaattgat
     1261 gatcgcttcg gattgtttga aagttctctc aatttccttg ggatctacct ggcgagcggc
     1321 ttgtcctagc ccacccggca acatatccat gatctgggcc agcggtccca tctttttcat
     1381 ttgcttcatt tggtcgagcc aatcttcaag cgagaattgc cccttcatca tgcgctcagc
     1441 cgttttttgt gcagtctgct gatcaaaggc ggcttcagct ttttcgatca aaccgatcat
     1501 atcgcccatg ccaaggatgc gcgaggaaag tcgagcgggg tcaaaggttt cgagcgcatc
     1561 gagcttttcg cccgtaccaa taaacttgat cggcacaccg gtcacggaac gaatagagat
     1621 ggccgcgccc ccacgtgaat caccatccat tttcgtcatc atcaaacctg tgatagatac
     1681 gttatcgcgg aagccctgag cgatattcaa ggcctcctga ccgatcatgg aatccacgac
     1741 cagtagggtt tcaacaggcg ggactttcgc cacaatggac tttaactcat tcatcaattc
     1801 cgcgtccaac tgcgaccgac cggctgtatc cacgatcatc acaccgaatc cacccttttc
     1861 ggctttctca aacgaacgtt tcacaaggtc aggaggcttg aggttcaaat ctgattcgac
     1921 gggcacatcc accctctccc ccagctgctg caactgcgtc actgccgccg gacgatatgg
     1981 atcgcctgca acgagcatca ctcgctcgcc ttttgagcgg agcaaacgcg ccaacttccc
     2041 agcgccggtc gttttaccag agccctggag acccaccatc atgatcacgc gtggctttgg
     2101 ccccgtgagg ttaagtggcg ccggctcgcc aagtgttgca attaattcat cattgacgat
     2161 tttgatcacc tgctggccag gattcagtgc cctggatact tcggcaccga ctgcgcgctc
     2221 gcgaacacgt gcgataaaag ctttaacgac actaaaatgt acatcggctt cgagtaaggc
     2281 gagccgtact tcgcgcatgg ccgcgtctac atccgcttca gaaagtttgc cgcggcggcg
     2341 gagttggtca aaaacatcgt tgagtcgatt cgtaaggttt tcgaacatat ttgttttcca
     2401 tcaaggccag gagcgaagcc cggaagccat ccgatatgca ctactgaaat tgatttgtag
     2461 ggctcctcgt ctcctcgcca tgaaacatga ttgagagtag gcgaagattg tagtgtgtaa
     2521 gcagatggag gtcaagagac gcctggtctc cagtgccctc cgtttcgcct gaggtgcttg
     2581 cttgacaata gttttgtcac aaagtaaaat cacgaacgct taacaacaga atatcgccga
     2641 gatagctcag ttggtagagc acgcgactga aaatcgcggt gtccacagtt cgattctgtg
     2701 tctcggcact ctcggctgaa cgttgagcca gaaattgcgg gcgtagtaca gtggtagtac
     2761 gtctccttgc caaggagaag gtcgtgagtt cgaatctcat cgcccgctct tgttttttct
     2821 ccatggcgtc gtggccaagt ggtaaggcaa gggtctgcaa aacccttatt caggggttcg
     2881 aatcccctcg acgcctctca tccgtatcct gttagaaagc ttcaagcaga cgccgtgagg
     2941 cgtctttttt gttgtttcat tgtagcttta tcgcaagttt tttgaacagg ttactttgtt
     3001 atataatgtc gcggcgccac ttcgcgacca tgcacggacc ccgcccatga aaatccttta
     3061 tgtggaagat gaaattgctc atgtcgtatt aacacagcga actctggaag acagtattca
     3121 gcatgagttc actttgttac acgctgaaac catcggcgat gcgctgcgga tactcgatgc
     3181 agaaccagat atcgatttga tactgagtga tttacgcctt ccagatggca cggggttgga
     3241 tttactgaaa aaagtacgtg aacgtgtagc agcgccggcc gttgtgctcg tcactgggca
     3301 gggagaccag gaggtggcag taacagctct caaggcgggg gcagccgatt atttggtcaa
     3361 gcaaagcgat tatctgcacc gattgccgat tgtcatcggc aacgccattg ctcaaaatcg
     3421 ttatttgcgc gaccaggccg cgttgcgtga ggcggaaatt aaatatcaat ccctcgttga
     3481 acagattcct gctgtggtgt ttttggaccg agcagacgaa tctgaagcca ctttctacat
     3541 gagtccgcgc atcgagagtc taactggctt tacgccccag gaatggtact caacggagaa
     3601 catatggttt gatcgcatcc acgccgcgga tcgcaaacga attgatgatg cctacgaaaa
     3661 atcacatgtc aacggaacgc gttttttcga ggaataccgc tttctacgac gcgatggtcg
     3721 aacgatctgg gttaaggagg acacaaatct cattcgggat gagatgggga atcccttgta
     3781 ctggcagggc atcctgctgg atattacgaa ggaaaaagaa tcccagacat tccttaaaga
     3841 aagtgaggag cgttttcgcc gtattttcca ttcatctcct attgcaactt gtgttgtgac
     3901 acttgaagat ggaaaattca tcgacgccaa tgaggcgttt cagaacttag tcggtattcc
     3961 gcttgagaat ttgattgggc atacctcagt ggaattgggc ttttggcgcg aaggcaccga
     4021 cagagagtct tttatttttc atttgcaaga tgaaagagcc ttgcagggcg tggaaattca
     4081 gtatacgaat gtgcccaacg ggccgaaaga tactttagcc tattatgagt taatcgaact
     4141 aggcggacaa tcctgcattc tgtctatgtt ttatgaaatc actgaacaaa agaaatcaca
     4201 gaaggcgctg cagcggcaac ttgaggaact atcggtgctg cattctgttg ctactgctgg
     4261 aacggaaagt tt
//
LOCUS       DASTJP010000566        10135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1100468, whole genome shotgun sequence.
ACCESSION   DASTJP010000566 DASTJP010000000
VERSION     DASTJP010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100468"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VFQ23_16530"
     CDS             <1..1439
                     /locus_tag="VFQ23_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9908256.1"
                     /translation="IGYLAQGMGFDANQTLQAAFHPNPVSQAELESEIASLASALSAN
                     PNDSLLQSKYDATLRQLSTTNDETPALLASLGLADLPPDTPIKHLSGGQKTRLMLARV
                     LFEEPHLLLLDEPTNHLDIEMLEWLEGWLNRFQGAALIVSHDRAFLDNTVTSVLELDS
                     DTHQNKSYPGNYSDYLEQKLAEREQQAQAWQDQQDEIAQLRSAATHIRSLTKMRKGGK
                     ADPRNTDGFSVGHFGNRATKRTAGRAKHIEARIEKLLTEDKIERPKQSWQMKLDFGAP
                     AHQSRDVLVTEKLSVGYTTHLPLLQDLNLHIRAGQRIALTGANGSGKTTLLRTIAGKL
                     NPLAGRLSLGGTVRLGYMTQEQEMLLPDLNALQSVQNVASFNETETRNFLHYFLFKGD
                     HALRKTSELSFGERARLQLGLLVAQGCTFLLLDEPINHLDIPSRARFEEALTNFKGTI
                     LAVVHDRYFIERFASDVWNVKDQKIEMY"
     gene            1451..3025
                     /locus_tag="VFQ23_16535"
     CDS             1451..3025
                     /locus_tag="VFQ23_16535"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HET9908257.1"
                     /translation="MIALAPTPYPDVNHILNLLFTDIKDILQAQLVGMYLFGSLANGD
                     FDQHSDIDVLVVTNGDIADDIFMALHDMHTRLAEIDSPWAVQQEVSYIPKNALRRFDA
                     GNNHHPHLDRDKGERLHMMTHESDWIIQRHLLRENGIVLTGPTLKTLIDPVTPQELRR
                     AVIDVLPLWVNPILADPGKIKGRGYQSYCVLSLCRMLYTLQNKAILSKRAAAGWGMET
                     LHRRWQPLIERAILGRKNSNQPADPDDINQTIEMMRYTLEVSKQPSIFPDVNEVLNML
                     LSKVKRILKEQFVGMYLYGSLSSGDFNPATSDIDFLVVTTDTLTPETVTKLEAMHQET
                     WATSLKRAGKLEGAYLPSELVCRHDPNGPLCPTVNEGKFYPDRPGSDWIIQRHVVREH
                     GVVLDGPDPKTLIEPVGPDEIRRAVMGILHEWWFPMLDDPSWLRDHDNGYRAFAVVTM
                     CRVLHALEYGTIVSKPKAIHWAGTQLDKLWKELIEKAAPVSPYNDVDIPLDKALNFIG
                     FTREQTLKNEANTNDP"
     gene            3015..3947
                     /locus_tag="VFQ23_16540"
     CDS             3015..3947
                     /locus_tag="VFQ23_16540"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET9908258.1"
                     /translation="MTHKVHLDTDLGGDIDDLCALAMLLHWEDVEITGITTVAEAKGR
                     RAGYIRHILGLESRNEIPVAAGADVSEGYFRYPELGYPDEARYWTKSIPAAPNPLEDA
                     LQLLKKSIEQNGTIIAIGPYTNLYLLDSQFPGILKHANVFLMGGYVYPIRAGFPQWSN
                     EMDWNIQLDVKSAKHVLENSSPTLIPLSVTAETALRRMYLDDLRKSGPIGELIAQQAE
                     AYAIDEQNEKKVGEVLEGPPNDIINFQHDGLACAIALGWNEGVEIEELPLVIEEQGGW
                     LHERVNASGKLMRVVTRVDGPRFNEFWLDKITRR"
     gene            3957..4715
                     /locus_tag="VFQ23_16545"
     CDS             3957..4715
                     /locus_tag="VFQ23_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908259.1"
                     /translation="MDSNRYELIAPRDLENYGNIANEIAGTSWPEFMLHDPIADEYWH
                     ELFDRFEEYQFALIDKETNQMAAMGNSVPFYWDRDLTELPEGGWDWVFVKAVEDHKKG
                     VQPNTQSAIQVAIRPEYQGQGLSTKMVNAMRSIGQSKGFETLVAPVRPNEKSKYPLIS
                     IDDYITWKTEDGLPFDAWLRVHARAGAKIIKPCHEAMTIRGTCTEWEEWTGIKFPQSG
                     LYTIPGALNPVEINIEHDQGIYVEPNVWLRHDLT"
     gene            4830..5618
                     /locus_tag="VFQ23_16550"
     CDS             4830..5618
                     /locus_tag="VFQ23_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908260.1"
                     /translation="MTKDGQGEYEMSTDRFQVITPATREDYRDLVRGLNKAVWPEFMR
                     HDAVASENWHELLDRFEEYQIALYDTEEKRVAAMGNSYPLRWESALDDLPDSGWDWSF
                     VEAVKQNGTNISPNMQCAIQVAVRGEYQGHGLSTRVLKALHAIGTSKGLNHLIVPVRP
                     NEKSKYPLIGMEDYITWHNEDSLPFDPWLRAHVKIGGKIVKVCHESKVIRGTPVEWEK
                     WTGLKFPQSGRYILPAALVPIEMNLEEDEGTYIEPNVWVSHTIT"
     gene            5662..6414
                     /locus_tag="VFQ23_16555"
     CDS             5662..6414
                     /locus_tag="VFQ23_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908261.1"
                     /translation="MNSQSHTRTIIAIIIFVGLLVLGYFYVFDPGQYESYLPVGISDF
                     RNRTVYQMDPQTILSSIDSGQTDVFSPQDSAAQIPEPQAVSWGQADFIKTVRALNQVV
                     WQEPMSGWRLYSMHFSTPCGDNISGFAEADFYYFKTTFRENGKIRYTTQNFSISPEYE
                     QAAWAGRATFPHPLIGWKYVRLNQLHVAAEDALAIAEANGGKETRQLVDNNCRIHVRL
                     AGDKTWQILIYQNDTGSSLFRMVIDPESGRIE"
     gene            complement(6444..7343)
                     /locus_tag="VFQ23_16560"
     CDS             complement(6444..7343)
                     /locus_tag="VFQ23_16560"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908262.1"
                     /translation="MTISYRIGRMEDSQSVFEVFVKAIMDYGKRMGVMAITGGDDPAV
                     VESLWQRRRSMFEFLAQDAAQFWVAERDGEIIAYARSIEHDGLQELTEFFVAPSHQSA
                     GVGNELLSRAFPITEARYRTIIATLDERALYRYMKAGVYGRFPFKYFYRKAEKVDIET
                     DLTIEPMQLDTHIEAINQIDRALLSHTRQPIHQWVKTTRDGFVYKRKKEIVGYGYVGS
                     SSGPFAVLDDNDFPAVLAHAESLMAEQGKEFGAQTPLINTKAIRYFIERKYQIDSFSA
                     IFMSNVPFGKFENYLGFSPEFFM"
     gene            complement(7443..7796)
                     /locus_tag="VFQ23_16565"
     CDS             complement(7443..7796)
                     /locus_tag="VFQ23_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908263.1"
                     /translation="MTLTGKQYGIILFTLATAFLHLSLMPYYGAQGRPFDPIVLNGLG
                     YLALLGAYFLPIPFFQQRHKQVWWALFGYTVLTLVLWLLLGNKDFSADVASSTGYYAK
                     AAEVVLLAFLWSDKP"
     gene            7996..8418
                     /gene="ssb"
                     /locus_tag="VFQ23_16570"
     CDS             7996..8418
                     /gene="ssb"
                     /locus_tag="VFQ23_16570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET9908264.1"
                     /translation="MYHTIIIVGNVGKDPEMRYTPSGQAVTSFSVATNRQYTTGNGEQ
                     VKETIWFRVTTWGKQAEVCNQYVKKGMKVLVEGRLTPDKATGGPRIWTKQDGSAGSSF
                     EVTAGTVRFLSGRGDGMEGPVMGGMESAEMPAEDDIPF"
     gene            8486..8857
                     /locus_tag="VFQ23_16575"
     CDS             8486..8857
                     /locus_tag="VFQ23_16575"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HET9908265.1"
                     /translation="MNTPSSQPTGQPSNPNANSSNQPAAPKINIPDGMEPAYTNLARI
                     SHSPADIVIEFAHMLPGESTANIRSRVVMSPLSAKLFLRALTENLARYEAAFGEINVP
                     VNNTLAENLFRPYHPPEPPKE"
     gene            8957..9652
                     /locus_tag="VFQ23_16580"
     CDS             8957..9652
                     /locus_tag="VFQ23_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HET9908266.1"
                     /translation="MHTLETIAERIRQKFDIRNAMRDQALAQARRLTRSCSLAIRAVH
                     RDDKEVMDSQLREARKLADNLRGELAEHPDLYHAGYSQDALKEFVEANVTCALIQNLP
                     FPTPEEIGVEESTYLNGLAEVVGELRRRIMDILRHGYSPEAERLLTIMDEIYAVLVTM
                     DYPDAITNGLRRQTDLARGIIEKTRGDVTFSLRSAHLSQAIRELSSQLRSGNGDENEE
                     NGSSIPPNADEDE"
     gene            9656..>10135
                     /locus_tag="VFQ23_16585"
     CDS             9656..>10135
                     /locus_tag="VFQ23_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908267.1"
                     /translation="MARAGHKFPLLVYQHMLNRWWPALITLGLGMFALAYSEYIDPMA
                     QFLPWRWQLFAGIGGLCILIGIFFIILRHLAYVQPFPDYLKLVTPFMRINISYKRIKK
                     TTTTEMHYLFPRKSMSGWVQDIFDPLAGRTAIVIDLNGYPISPRMLRVFLSRFFFKDK
                     "
BASE COUNT         2786 a         2518 c         2430 g         2401 t
ORIGIN      
        1 ggattggata tcttgcgcag ggcatgggtt tcgatgccaa tcaaacgctt caggccgcat
       61 ttcacccaaa tcccgtttca caagctgaac tcgaatctga aattgcttca cttgcctctg
      121 ccctatccgc caacccaaac gactccctcc tacaatccaa atacgatgcg acgcttcgtc
      181 agctctccac caccaacgat gagacccctg ccctgcttgc ttcattaggg cttgcagatc
      241 tcccacccga taccccaata aaacatctca gcggtgggca aaagactcgc cttatgctgg
      301 cgcgggttct gttcgaagaa cctcatcttt tattgctcga cgagcccaca aatcatctcg
      361 acatcgaaat gcttgaatgg ctggagggct ggctcaatcg ctttcagggt gcggcgctaa
      421 tcgtttcgca cgatcgtgct tttttggata acacagtcac atccgttctt gagttggact
      481 ctgatactca tcagaacaag tcgtatcccg gtaactacag cgattatctt gagcagaagc
      541 tggccgaacg cgagcaacag gcccaagcct ggcaggatca acaggatgag atcgcccaac
      601 tccgttcggc ggcaacgcat attcgcagtc tcacaaaaat gcgcaagggt gggaaagcag
      661 atccaaggaa taccgacggc ttttcggtcg gacactttgg taatcgcgcc acgaagcgga
      721 cggcggggcg cgcgaaacat attgaagctc gcatcgagaa actcctcaca gaagataaga
      781 tcgagcgacc aaaacaatcc tggcaaatga aattggactt cggcgcgcca gcgcaccaat
      841 cgagagatgt attggtcact gagaagctat ctgttggcta cacgacacat ctgcctctgc
      901 tccaagatct caacctgcac atccgcgcgg gtcaacggat cgcgctcact ggcgcaaatg
      961 gaagcgggaa aacgacatta ctccgtacaa tcgctggcaa attgaatcca cttgctggca
     1021 ggctgagctt gggtggcact gtaaggctcg gttacatgac tcaagaacag gaaatgctgc
     1081 ttccagatct taatgccttg caatctgtcc agaatgttgc atcctttaac gagaccgaga
     1141 cacggaactt tctgcattat ttccttttta aaggcgacca tgcactgcgc aaaacatctg
     1201 aactttcgtt tggggaacgc gcccgcctgc aactgggatt gctggtcgca caaggttgca
     1261 cgttcctatt gctggatgaa cccatcaatc acctggacat tccgtcgcgt gcacgctttg
     1321 aagaggccct gacaaatttc aaaggaacca tcctcgccgt cgtgcatgat cgttacttca
     1381 tcgaacgctt tgcttctgat gtttggaatg tcaaggatca aaagattgag atgtactaag
     1441 gaaagcaaga atgatagctc ttgcccctac tccctatcct gatgtaaatc acattctgaa
     1501 tctgttgttc actgatatca aagatatctt acaagcccaa ctcgttggaa tgtatctatt
     1561 cggttctctg gcgaatggcg actttgacca acacagcgat attgatgtcc ttgttgttac
     1621 gaatggagac attgcagatg acatctttat ggcacttcat gatatgcaca cccgacttgc
     1681 agaaatcgat tcgccctggg cagtgcaaca ggaagtatct tacataccca aaaatgctct
     1741 tcgccgtttc gatgctggca acaatcatca ccctcatctt gaccgggata aaggcgagag
     1801 gcttcacatg atgacccacg aaagcgactg gatcatccaa cgtcatctct tacgtgagaa
     1861 tggcatcgtc cttacaggac ccactctaaa aactttgatc gaccctgtta cgccgcaaga
     1921 actgaggcgg gccgtaatcg atgtattacc tttgtgggtc aatccgatac ttgctgatcc
     1981 aggcaagatc aaaggtcgcg ggtatcagtc gtattgcgtc ttgtcgcttt gcagaatgct
     2041 ttacacgctt caaaacaaag ccattctatc caaacgcgcg gccgcgggct ggggaatgga
     2101 aactctacac aggcgttggc agcccttaat cgaacgagcc atccttggac gtaagaactc
     2161 taatcaaccc gccgaccccg atgacatcaa ccaaacaatc gagatgatga ggtataccct
     2221 tgaggttagt aagcagccat ccatatttcc ggacgtaaat gaagtgttga acatgctact
     2281 ctcaaaagta aagagaattc tgaaagaaca attcgtcggc atgtatctct atggctcact
     2341 ttcaagcgga gacttcaacc cagcgacaag tgatatagat tttctcgttg tcactaccga
     2401 tacactaacg cccgaaacgg tcaccaaact cgaagcgatg catcaagaga cgtgggcgac
     2461 gagtctcaaa cgcgctggca aactcgaggg cgcgtaccta cccagcgagc tcgtatgtcg
     2521 ccatgatcca aatggtccgc tgtgcccaac cgttaacgaa ggaaaattct accctgaccg
     2581 cccgggcagt gactggatca ttcaacggca tgtggttcgt gagcatggcg tcgtgttgga
     2641 tggacccgat ccgaagacat tgatcgaacc agttggtcct gacgaaattc gccgtgccgt
     2701 tatgggcata ttgcacgagt ggtggtttcc aatgctcgat gacccatcct ggctaagaga
     2761 tcatgataat ggatatcgtg cctttgccgt cgttaccatg tgccgcgtcc tgcacgcact
     2821 cgaatatggg accattgttt ccaagcccaa agccatccac tgggcgggca cccagttgga
     2881 taaactctgg aaggaactga tcgaaaaggc tgctccggtt tcaccctaca atgacgtaga
     2941 tattccgctg gacaaggcat tgaattttat cggttttaca cgtgaacaaa ctttgaaaaa
     3001 cgaggcaaac acaaatgacc cataaagtgc atctcgatac agacctcggc ggggatatcg
     3061 atgatctctg tgcgcttgcc atgttgcttc actgggaaga tgttgaaatt acgggcatca
     3121 ccaccgtggc agaggcgaag ggtcgcagag ccggatacat tcggcacatc ttaggactcg
     3181 agagtaggaa cgagattcca gtggcagccg gagcggatgt gtcagaaggg tattttcggt
     3241 atcccgagtt gggttatccg gatgaggcga ggtattggac aaagtcaatt cctgctgcgc
     3301 cgaatcccct tgaggatgcg ctgcaattgc tgaagaaaag tatcgagcaa aacgggacaa
     3361 tcattgccat cggtccatat accaatttat atttattgga tagccaattc cccggaattt
     3421 tgaagcacgc taatgttttt ctcatgggag ggtatgtgta tccaatccgg gcgggctttc
     3481 cgcagtggag caacgaaatg gactggaaca ttcagcttga tgtgaaatcg gcaaaacacg
     3541 tcctggaaaa ttcaagtcct actctcattc ccttatcagt aactgcggaa accgccctgc
     3601 gcagaatgta tctggacgac ctacgaaaat ccggtccgat aggagaacta atcgcacagc
     3661 aagccgaagc ctacgccatt gatgaacaaa acgaaaaaaa agtaggcgag gtccttgaag
     3721 gaccacccaa cgacatcatc aattttcaac atgacggatt agcctgtgcg atcgcgctcg
     3781 gttggaatga aggggtggaa atcgaggaat taccgttggt catcgaagag cagggtggat
     3841 ggcttcacga aagagtcaat gcatcaggaa agctaatgcg cgtcgtaact cgcgtcgatg
     3901 gaccgcgctt caacgaattc tggctggata aaattacaag aaggtaaaat acaaatatgg
     3961 actcgaatcg ttatgaatta atcgcaccac gtgacctgga aaactatgga aacattgcca
     4021 atgagatcgc tggaacatcc tggccagagt ttatgctcca tgacccgatt gccgatgaat
     4081 attggcacga gctttttgat cggtttgaag aatatcaatt tgcgttgata gacaaagaga
     4141 ccaaccagat ggcagccatg ggaaacagcg ttccattcta ctgggatcga gacctgacgg
     4201 aactgcctga gggcggttgg gactgggtct tcgtcaaagc tgtagaagac cataagaaag
     4261 gcgttcagcc caatacgcag tctgcaattc aggttgcaat ccgtcccgag tatcaggggc
     4321 aaggtttgag caccaaaatg gtaaacgcaa tgcgctcaat tgggcaatcg aagggattcg
     4381 agaccctggt tgcccccgta cgccccaatg aaaaaagtaa gtatccgctc ataagcattg
     4441 atgattacat tacctggaaa actgaggatg gcttgccgtt tgatgcatgg ctccgagttc
     4501 atgctcgagc gggagcaaaa atcatcaaac catgtcacga agccatgacg atccgcggga
     4561 cctgcactga atgggaagaa tggacaggga tcaaatttcc tcaaagcggt ctttacacca
     4621 taccaggagc cctgaacccg gtcgaaatca atatcgagca tgaccaggga atctacgtgg
     4681 aacctaatgt ttggctccgg cacgacctaa cataggacca aaatcttcat taatccttaa
     4741 tcgctcaaat cattgaaact atgtgacaga cccttcagag tcggccttca tcctatacaa
     4801 tgaattacaa ttcctgacca ggatgcttat tgacaaaaga tggtcagggc gaatatgaga
     4861 tgagtacaga tcgctttcaa gttatcactc ctgccacgcg tgaggactat cgtgaccttg
     4921 tgcgcgggct taataaggct gtctggccag agtttatgcg acatgacgcg gtcgcttcag
     4981 aaaactggca cgagctgttg gatcgcttcg aagaatatca aattgccttg tatgataccg
     5041 aggagaaacg cgtcgcggcc atggggaata gttacccgct acgatgggag tccgcgctgg
     5101 atgacctgcc cgatagcggc tgggattggt cctttgtcga agctgtaaaa caaaacggta
     5161 caaacatatc gcctaacatg cagtgcgcta ttcaggttgc agttcgtgga gagtatcagg
     5221 ggcacggctt gagcacgcgc gttttaaaag cactacatgc gatcggaaca tctaaaggat
     5281 tgaatcacct catcgttcct gtaaggccga acgagaagag caaatatcca ctgatcggta
     5341 tggaagatta catcacctgg cacaacgagg actcgctgcc cttcgacccc tggctgagag
     5401 cccatgtgaa aattggcggc aaaatcgtta aagtgtgcca tgagtcaaag gtgattcgag
     5461 gtacgcccgt cgaatgggag aaatggaccg gactcaaatt tccacaaagc ggacggtata
     5521 tccttcccgc ggcgctggtg ccaattgaaa tgaatctgga agaagatgaa gggacatata
     5581 tcgaacccaa tgtgtgggtg tctcatacaa tcacttagga tgttacagga agaattaagc
     5641 cacgctaatt aaggagtaag gatgaactct caaagccata ccaggacgat catcgcgata
     5701 atcatctttg ttggtctcct cgttttaggt tatttttatg tgttcgaccc cggccaatac
     5761 gaaagttatt taccggttgg aatcagtgat tttcgaaacc ggacggtata ccagatggac
     5821 cctcagacga ttctttcatc cattgatagt gggcaaacag acgtcttttc accgcaggat
     5881 tcagccgccc agattcctga gccacaagcc gtttcatggg gacaggcaga tttcatcaag
     5941 acggttagag ccttaaatca ggtagtatgg caagaaccaa tgagcggttg gagattgtac
     6001 tccatgcatt tttccactcc ctgcggggac aatatcagtg gttttgcgga agctgacttt
     6061 tattatttca agaccacctt ccgcgagaat ggaaaaataa gatataccac tcaaaatttt
     6121 tccatatcac cggaatatga gcaggcagct tgggcaggac gcgcgacctt tcctcacccg
     6181 ctaatcggct ggaagtacgt ccgattgaac caactccatg tcgcagcaga ggatgcgctt
     6241 gcaattgctg aagcgaacgg tggtaaagaa actcgacaac tagtcgacaa caattgccgg
     6301 atccatgtgc gacttgcagg cgataagacc tggcaaatcc ttatctacca aaacgatacc
     6361 ggatcatctc tgttcaggat ggtaattgac ccggagtccg gcaggattga ataaattaaa
     6421 aattattttt aaagtagctg atcttacata aaaaactcgg gactaaaccc aaggtaattc
     6481 tcaaatttcc cgaatggcac attgctcata aagatcgccg agaaagaatc gatctgatat
     6541 ttgcgttcga tgaagtatcg aatcgctttc gtattgatta acggtgtttg agccccaaac
     6601 tcttttccct gttctgccat caggctttca gcatgcgcca aaaccgccgg aaaatcgtta
     6661 tcgtcgagaa ctgcaaacgg tccgctactg gagcctacat agccatagcc gacgatttct
     6721 ttctttcgct tatagacaaa tccatcacgg gttgttttta cccattgatg aatcggctgg
     6781 cgtgtatgac tcaagagagc cctgtcaatt tgatttatag cttcgatatg ggtatctagc
     6841 tgcatgggtt caattgttag atcggtttca atatccacct tttctgcctt gcgataaaaa
     6901 tacttgaacg ggaagcgacc atataccccg gctttcatat atcgataaag tgcgcgttca
     6961 tccagggtgg caatgatcgt ccggtagcgc gcctccgtta tgggaaaggc gcgtgacagc
     7021 aactcgttcc ctacgcctgc tgattggtga ctgggagcta cgaaaaactc ggttaactcc
     7081 tgcaacccat catgttcaat cgaacgtgcg taggcaatga tctctccatc gcgctctgcc
     7141 acccagaatt gcgccgcatc ctgtgcgaga aattcaaaca tggatctacg tcgctgccaa
     7201 agcgactcta caactgccgg gtcatcccca ccggtaattg ccatcacacc catgcgtttg
     7261 ccgtaatcca taatggcttt tacaaatact tcaaaaacgg attgggagtc ttccattcgc
     7321 ccaatccgat agctgatggt catgtttcaa atcctttgaa tcgtttcagg atcttctctg
     7381 gcaaaccaac aacacccctc gatcatccat tattcttaat gtatctcgaa aataagcgta
     7441 gcctatggtt tatctgacca gaggaatgcc aataagacaa cttcagcggc tttggcgtaa
     7501 taaccagtag agctggcaac atctgcagaa aaatccttgt tccccaaaag aagccacagg
     7561 accaacgtga gcacagtata gccaaacaat gcccaccaga cttgcttatg cctttgttga
     7621 aaaaatggta tcggcagaaa gtaggcaccc agcagggcaa gatatcccaa gccatttagg
     7681 acaatcggat caaaaggtct gccctgagca ccgtaatacg gcatcaatga aagatgcagg
     7741 aaagctgtcg ccaaagtgaa aagtatgatc ccatactgct tgccggtcag tgtcattgtt
     7801 atctcctctt aagggaataa atacctatgc caaaacttac gaaaccgacg taagtttgga
     7861 tcttacaaat agaacactcc gtcagccttt ttgtttcgct ggttaacaaa tcaacgaaga
     7921 acgggtaaaa tatggattgt tcatttgcac cccgtcggca aatcggcggg tacctcaact
     7981 caaggagatt ttcccatgta tcacaccatc attatcgtcg gaaatgtagg caaagacccc
     8041 gaaatgcgtt atacgccttc aggtcaggca gttacttcgt tttctgtagc taccaaccgc
     8101 caatatacca ctggcaatgg cgagcaagta aaagaaacga tttggtttcg cgttacaacc
     8161 tggggaaagc aggcagaagt ttgcaatcag tatgttaaaa aaggaatgaa ggtattagtg
     8221 gaaggtcgcc tcactcctga taaagccaca ggcggaccac gcatttggac caaacaggat
     8281 ggctccgctg gttcttcctt cgaggtaacc gcaggtaccg tgcgcttcct ctccgggcgt
     8341 ggtgatggta tggaaggtcc ggtaatggga ggcatggaaa gcgctgagat gcctgctgag
     8401 gatgatattc cattctaatg cccaactctt tccgagagct tcgcataaat ttattaatag
     8461 ccaagggaga gaggaatgtt agactatgaa cacaccttct tctcaaccaa caggtcagcc
     8521 cagtaatcca aatgccaata gttccaatca accggctgca ccaaaaatca atatcccaga
     8581 tggaatggag cctgcctaca caaacctggc acgcatttca cactcacctg ccgatattgt
     8641 tatagagttt gcgcacatgc taccagggga atctacagca aatatacgct ctcgtgtggt
     8701 gatgagcccc ttgagtgcaa aattatttct acgagcactc accgaaaacc tggcgcggta
     8761 cgaagctgcc tttggtgaga tcaatgttcc agtgaataat acgcttgcag agaatctgtt
     8821 ccggccctat catccgccag agccgccaaa agaataagtc agtatcgcag ggggcgagcg
     8881 atttcctatg gcaaaacatg tacgtcttca atcaagggcc gccctttaac gtccacactt
     8941 tgggagcgca gtctttatgc acacactcga aacaatcgct gaacgaatac gccaaaaatt
     9001 cgatatccgg aatgccatgc gagaccaggc acttgctcag gcacgcagac taacgcgctc
     9061 ttgttcattg gcgatccgcg cggtccatcg ggacgacaag gaggtaatgg attctcaatt
     9121 gcgggaagca cgcaagctgg ccgacaacct tcgtggtgaa ctcgcggaac acccagacct
     9181 gtatcacgca ggctattcac aggatgccct caaagagttt gtagaagcca atgtgacctg
     9241 cgctttgatc caaaacctcc cttttccaac acccgaagaa atcggcgtgg aggagtcgac
     9301 ctatctaaac ggcctggctg aggttgtggg cgagcttcgg cgacgaatca tggacatcct
     9361 gcggcatggc tattcaccgg aagccgaaag gctactcacg attatggatg agatttatgc
     9421 cgttctggtt accatggact atcctgacgc gatcacaaac ggcttacgcc gtcagacaga
     9481 tctagcacgc ggcatcatcg aaaagacgcg cggcgatgtg accttcagcc tgcgcagcgc
     9541 acatctgtca caagccatcc gggagttgag cagtcaatta aggagtggga acggtgatga
     9601 gaacgaagaa aatggatcat caattccgcc aaatgcagat gaggatgagt aattaatggc
     9661 acgtgccgga cataaatttc cgcttctcgt atatcaacac atgctgaatc ggtggtggcc
     9721 ggcattgatc acgctggggc ttggaatgtt cgccctggct tacagtgaat atatcgaccc
     9781 aatggcacaa ttcctgccgt ggagatggca gttatttgca ggaatcggcg gattgtgcat
     9841 tttgatcggc attttcttca ttatcctgcg acatctggcg tacgtccagc ccttcccgga
     9901 ctatttgaaa ctagttacgc cgtttatgcg aatcaacatt tcctataagc gcattaaaaa
     9961 gacgacaacc accgagatgc attatctgtt cccacgcaag agcatgtctg gctgggtgca
    10021 ggatattttt gatcccctgg ccggccgaac ggcaattgtg atagacctga atgggtaccc
    10081 catctctcca agaatgctgc gcgtctttct ttcgagattc ttttttaagg ataag
//
LOCUS       DASTJP010000567         7531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_364306, whole genome shotgun sequence.
ACCESSION   DASTJP010000567 DASTJP010000000
VERSION     DASTJP010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364306"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..991
                     /locus_tag="VFQ23_16590"
     CDS             269..991
                     /locus_tag="VFQ23_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908268.1"
                     /translation="MNKRTSLWMLAIILLTSCLGQSQESITPTPSITSTQTLIPPTLT
                     STPTNTPSPTETNTVTPEPFGCLKPPDDYTQVEVNNSTLNQRTLSMLGNAQALYGGEI
                     ELTGHAITQGSYSNNVAASFGTHSGGGAVDLSVLQRGTYTVLWDDIEPVLRALRAAGF
                     AAWLREYGELHPDSPIHIHAIAIGDQELSPAAQEQLIGEAGYFRGFSGLPVSLYGPPT
                     PDRYGGPVICQWMIDLGYRDLR"
     gene            1266..1610
                     /locus_tag="VFQ23_16595"
     CDS             1266..1610
                     /locus_tag="VFQ23_16595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9908269.1"
                     /translation="MQITVREFKEMVVIAITERIDGSTAPEVENVIESVQHRGRYKIV
                     IDMSQLAYISSAGYRVLLSAQRNCKRFDRGEVILASVPEQIRQSLDISGFTDYFRIYN
                     EISAVPELSERP"
     gene            1807..2385
                     /locus_tag="VFQ23_16600"
     CDS             1807..2385
                     /locus_tag="VFQ23_16600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9908270.1"
                     /translation="MKNLKAGLIQEEKEKGQAESNANASGFEALFVEHWGPVYRVLQR
                     LVGDPSEAEDLALETFFRLYKRYPPRESEFNIGGWLYRVATNLGLHSIRSWKRRELYE
                     TKAGKYALEATPEARPAEILAKAEEQRVVRLALAKMNEQRSQLLIMRHSGMSYKEIAE
                     ALGLAPTSIGPLLIRAEREFEKHFRAMTQEEP"
     gene            2382..3545
                     /locus_tag="VFQ23_16605"
     CDS             2382..3545
                     /locus_tag="VFQ23_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908271.1"
                     /translation="MSTHLTDGELRAALDGELDSVQLEHLHTCTACQARQEQLQSKSQ
                     EAARLLSSLSTTDQPAPNTQRAWSRFSQQLITKKESSMLRKWFAFPVVRYASALVLIL
                     TLVLAFPSTRALAGELLKLFRIQQVAVVSVDFTGLEQLTGDGVLGNQFTDLISNSMDI
                     TKEPGEPVEAADAAQASQLSGFSVRLPQTASPSHIYVTDGAAFTMTVDRAKAQALLDE
                     AGRGDLVLPESVDGADISVNIPSAVSAAYGTCPKPDVDSAAAEDSGQTYRDCVILAQI
                     PSPTVNAPADLDINRLAQIGLEFTGMTSEEAAAFTSTVDWTSTLVVPIPSNAANYEQV
                     TVDGVTGTLIRRASREGEPTTFALFWVKDGIIYAISGRGANSDLAIEMANSLP"
     gene            3598..4515
                     /locus_tag="VFQ23_16610"
     CDS             3598..4515
                     /locus_tag="VFQ23_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908272.1"
                     /translation="MTIPAIKTEELRKVFGDRAAVKGLTLQVEQGEVFGFLGPNGAGK
                     TTSMKMLLGLVAPTSGNASLLGAPIGDIPTRARVGFLPEHFRFQDWLSAREFLRLHGQ
                     LLGMESRDLQTRLEQLLERVGLSPFGNKLLRTFSKGMLQRVGLAQALLNRPALVFLDE
                     PTSGLDPVGRRLVRDIIRELKEQGTCVFLNSHLLSEIEITCERVAFISHGEVIRVIEM
                     DSLEKNMSRVTIRAGGLTPESVAGLARWGENIQSNDGQLVMTLHQERDLPEITRYLVA
                     QKAEVYAITPNRLSLEDIFIETIGEDGGL"
     gene            4512..5366
                     /locus_tag="VFQ23_16615"
     CDS             4512..5366
                     /locus_tag="VFQ23_16615"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908273.1"
                     /translation="MNSISVLARMTFQEAIRRRIVLTGLVLGILFLIVFSVGSQMIST
                     EIERETIAEGGNAYQNVMRTEFSNFLLMAGLYAVTFLSVAMGALLGADTLAGEINSGS
                     IQTILTKPIRRSDVVFGKWLGFAGLLGLYALFMAGGVVTIVFFQSDYIPRNILAGLSL
                     IYLESLVVMTISLAGSSAMPALATGGMVFGLYGLAFLGGWIEQIGSFMQNPTTLQIGI
                     ITSLIIPTESLWRRAAYEMQTPLASTIGISPFSAFSVPSLLMVAYAVFYLILTLIIAV
                     NTFQQRDI"
     gene            5604..7337
                     /locus_tag="VFQ23_16620"
     CDS             5604..7337
                     /locus_tag="VFQ23_16620"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9908274.1"
                     /translation="MSHIFISYSRRDLTFAQKIVDALAAQNVEMWIDWKSIPKGEDWE
                     QEIYRGIEAADALLFLISPDSIRSKMCNTEIIHALKSGKRILPVLLKKSTTSEFLDET
                     AKREIERLNWVFIQKDIDDFDGAIEQIRKTIRTDYDWVRFHTTLLTKALAWERAGEDA
                     SRLLRGKELAEAEKQIADSGPATEPFLTDLQRQFLTKSRQSVKRRQALPYSVLALLAA
                     TIFLGGKFFYLILPISSACPEVGSVSFEIPRAKLTESTYQTLLEAIENGPSKTARQNC
                     ELGAEELIYVTFGDSSAGDQIELSVRLPEIPAYQLDLLQEIREFGPELVEQSEAIALL
                     NAFSAYSVGEYQTSIDLLTGYDSLSSLTLLAQAQLFRDDLGASREAYDLALQKTQPDS
                     VYTGKLNMGAALALWRPESYYLLSFEGKKEDCKAAAIYYSHAMDQFEADKLAHNVRIL
                     YANYCFTDNDKTVPGLEQYAVWKDEAPQSESDLQSKDATYINATEQYILALRERMNKG
                     PTLQYRNHLISAQPLMLARAALSDFYWKVDENCAEARVSRDEFRSSIYSGIEKWSLER
                     LLQSQPFFCRS"
BASE COUNT         1915 a         1890 c         1924 g         1802 t
ORIGIN      
        1 ttgattttgt atcgatggtg ttgttctttc atacaacact agttaaacta taaaacctga
       61 ttgttaaaaa cgtacaaaac caagataaaa atttcgtcag aattcgccgt acacaattta
      121 gattattttc atctgccccc acccatcctc ccccaaatcc gcaagtattg ggggaggtgc
      181 ccaacacttg cagctggcgc acaccgcccc ggttggtcgg tgccggggag tgcaggtgag
      241 agcgcagggg tcagaatata atgaacgcat gaataaacgc acttcattat ggatgctagc
      301 aatcatctta ttaacaagtt gcctgggcca gtcgcaggag agtattactc ccaccccttc
      361 gatcacttct actcagacac taatcccacc tacactcact tcgactccaa ccaatacgcc
      421 ttcccccacc gaaacgaaca cagtcacgcc cgaaccattt ggctgcctca aaccacccga
      481 cgattacacg caagtcgaag tgaacaactc gactctgaat cagcgcacgc tgtccatgct
      541 cggcaacgct caggcgttat acggcggaga gatagagctc accggccatg ccattacaca
      601 aggtagttat tcgaataacg tcgcggcatc gtttggtaca cacagcggcg gcggagccgt
      661 ggatctctct gtattacagc gcggcacgta tacggtgctg tgggatgaca ttgaacccgt
      721 tctccgtgct ttgcgtgcgg caggctttgc ggcctggctg cgagaatacg gtgagcttca
      781 ccccgactcg cccattcaca tccacgcgat cgccattggg gatcaggaac tctcgcctgc
      841 cgcgcaggaa caactcatcg gcgaggcggg ttacttccgt ggtttcagcg gtttgccagt
      901 ctcgctatac ggacctccca ctccggatcg ctacggcgga ccagtcatct gccagtggat
      961 gattgacctg ggctatcggg atttgcgata ggtcatcatc ccaaatccga caatagggga
     1021 tttactctga agaggcagtg caaaatcgta tttggagacg gtatatagtg ggaatttagt
     1081 cctattcttg caacgcagtt ggatttgttg ggttaactac agagtgcatc cttcggataa
     1141 tttcttatgc tattgtcgca agatgtacca gaagtggaat tgaaaattaa attgtgagac
     1201 agcactagga agtattcata acaaaacgcc gatcaaatca agataagaaa agggaagtag
     1261 caaacatgca aatcactgtc cgtgaattca aggaaatggt cgtcatcgct attacagaac
     1321 ggatcgacgg atccactgcc ccagaggttg aaaacgtcat tgagtctgta caacatcggg
     1381 gcaggtataa aatagtgata gacatgagcc agttggcata tatatccagt gccggatacc
     1441 gtgttttgct gtctgcccag cgcaactgca aacgctttga ccgcggtgaa gtgatactag
     1501 cttcggtacc ggaacagatc cgccagtcac tggatatcag cggctttacc gactatttta
     1561 ggatttacaa tgaaatctcg gctgttccgg agttatcaga gagaccctaa aggttaatat
     1621 tgcaaatgaa cattctgatt taaccaggct ttccatacaa accttttgtc cgatgtaact
     1681 gaaaaccttt agggtctaca gaaacaatat tcgctgtctt atttccagct cgaggggggt
     1741 tatatatata gaatcgattc ttatggctgc aaacaaaatg tgttccaaca gagtatgatt
     1801 ggctcgatga aaaatctcaa agctggcctg attcaggaag aaaaagaaaa gggtcaggct
     1861 gaatcaaacg caaacgcttc cgggttcgag gctctgttcg tggaacactg ggggccggtc
     1921 tatcgtgtcc tgcaacgcct ggttggcgac ccgtcagaag cagaagacct ggcactcgaa
     1981 accttcttcc gcctgtacaa gagatatccg ccccgcgagt ccgaatttaa catcggtggc
     2041 tggctctacc gggttgcgac caatctcgga ttgcattcca tccgtagctg gaaacgtcgc
     2101 gaactttatg agacgaaggc cggcaaatat gctctcgagg caacaccgga ggctcgcccg
     2161 gcagaaatcc tggcaaaggc cgaggagcag cgcgttgtcc gactggcact ggcaaagatg
     2221 aacgaacagc gatcacaact tctgatcatg cgccattccg gaatgtccta taaagagatt
     2281 gcggaagccc tcgggcttgc gccaacgtca atcggtccat tgttaatacg ggccgagcgt
     2341 gaatttgaaa aacacttccg tgcaatgaca caggaggagc catgagtaca catttgaccg
     2401 atggtgagct gcgcgccgcg ctggacggag aactggattc tgttcaactc gagcatctgc
     2461 atacctgcac ggcctgccag gctcggcaag aacagctgca gtcaaaaagc caggaagccg
     2521 cgcgcttgct ctcgtctctt tcgaccacag atcagccggc tcccaatact caaagagcct
     2581 ggagtcgatt ctctcaacaa ttgataacga aaaaggagtc ttccatgtta agaaaatggt
     2641 ttgcttttcc cgtcgtacga tatgcatctg ccctggtctt gattcttaca ctcgtgctgg
     2701 cattcccaag cacgcgcgcc ctggcaggtg aattattgaa gctgttccga atacaacaag
     2761 tggctgttgt ttcagtggac tttacaggtt tagagcaatt gaccggtgat ggtgtgctcg
     2821 gcaaccagtt cacagacctg atctcaaatt cgatggacat tacaaaagaa ccgggcgagc
     2881 ctgttgaagc ggcggatgca gctcaggcca gccagctctc cgggttcagc gttcgattgc
     2941 cacagactgc cagtccatcg cacatttacg tcacagatgg cgccgccttc acgatgacgg
     3001 tcgatcgcgc aaaagcacag gccctgctcg atgaagccgg gcgtggagac ctcgtcctgc
     3061 ccgagtctgt agatggggct gatatctcgg tgaacatccc ctcagcggtc agtgcagcgt
     3121 atggaacctg ccccaagcct gacgtagata gcgcggcagc cgaagactct ggccagactt
     3181 atcgagattg tgttatcctt gcgcaaatcc ccagccccac tgtcaacgca cctgcagatc
     3241 tcgatattaa tcggctggcg cagatcggcc tggaattcac cggtatgacc tcggaagaag
     3301 ctgctgcctt caccagcact gttgattgga cttccacgct ggttgtgccc atcccgagca
     3361 acgccgcgaa ctacgaacag gtcacggtgg atggtgtcac gggtacgctc attcggcgtg
     3421 cttccagaga aggagaaccg acaaccttcg ctctgttctg ggtcaaggat gggatcatct
     3481 acgccatcag cggccggggt gccaactcgg atttggcgat cgagatggca aattcactgc
     3541 cctagtgata aggtgactgt cacggtgaac gtgacggtca ccttatgata cgaatttatg
     3601 actatccctg caattaaaac agaagaacta agaaaagtat tcggcgaccg agccgctgtg
     3661 aaaggcctca ccctgcaagt agagcagggt gaggtattcg gttttctcgg gccgaatgga
     3721 gccggcaaaa cgacctccat gaaaatgcta ttggggttgg tggcgccaac ctccggcaac
     3781 gcgtccttat tgggcgctcc gattggcgat atcccaactc gtgctcgcgt tgggttcctc
     3841 ccggaacact ttcgttttca ggattggctg agcgcccgcg aattccttcg gctgcatggc
     3901 caattacttg gcatggaatc tcgggatctg caaacacgct tagagcagtt gctcgagcgc
     3961 gtaggactct caccgttcgg aaataagttg ttgcgcacat tttcaaaagg catgcttcag
     4021 cgcgtggggt tagcacaggc attgttgaac cgtccggcat tggtctttct ggatgagcca
     4081 acttccgggt tggatcccgt cggtcggcga ctggtgcgcg acatcatccg cgaactcaaa
     4141 gaacaaggga cttgcgtctt cctgaactct cacctgctaa gtgagatcga aatcacttgc
     4201 gaacgcgtgg cgttcattag ccatggcgaa gtgatacgcg tgatcgaaat ggactctctt
     4261 gagaaaaata tgtcgcgtgt caccatccgt gcgggagggc ttacgccaga atcagtggcc
     4321 gggcttgcgc gctggggcga aaacattcag tcgaacgatg gacagttggt catgaccctg
     4381 catcaggaac gtgatctgcc ggagatcacc cgctatctgg ttgcacagaa ggcggaagtc
     4441 tatgcgatta cacccaatcg cctgtctttg gaagatatct ttatcgaaac aataggagag
     4501 gatggcgggt tatgaacagc atttctgttt tggcgcgtat gacgttccag gaggccatcc
     4561 ggcgtcgtat tgttctgacg ggcctggtcc tgggtattct ctttctgatc gttttttccg
     4621 ttggctctca gatgatttct acagagatcg aaagagagac aattgctgaa ggcggcaatg
     4681 cctaccagaa tgtgatgcgg acagagttct ccaacttttt attgatggca ggcttgtatg
     4741 cagtcacgtt tttgtctgtt gccatgggag ccctcctcgg cgcagataca ttggcaggcg
     4801 agatcaattc cggatcgatc cagaccatcc tcaccaaacc catccgtcgc tctgatgtgg
     4861 tgtttggcaa atggttgggt tttgcaggtc tgcttggctt gtacgccttg ttcatggctg
     4921 gaggcgtcgt aaccattgtg ttcttccagt ctgattacat acctcgtaac atcctggcgg
     4981 gactctcgct gatttacctg gagtctctgg tggtcatgac catctccctg gcaggttcca
     5041 gtgccatgcc ggcgctggcg accggaggca tggtgtttgg tctctacggg ctggcattcc
     5101 ttggtggttg gatcgagcaa atcggttctt ttatgcaaaa cccgaccaca ctgcagatcg
     5161 gcattattac aagcctgatc atccccactg aatccctttg gcgacgagct gcctacgaga
     5221 tgcaaacccc tctcgcaagc acaatcggga tatcgccttt tagtgcgttt tcggtcccca
     5281 gtttattgat ggttgcgtac gcggtcttct atctcatcct gacattgatc atcgctgtca
     5341 acacatttca acagcgagat atttaacccg tcccaacgtc cccaaatccg acatagaaat
     5401 aatgaatgtg aagagaagtc tctttgtcgt atttggggac tgcccagcgt acccaagcag
     5461 agcgagtggt aggaagggta gggctggtag gttcttttta tttaagcaaa aggcttgcat
     5521 tcacgattaa tttcctgtat gcttgaacac atcaacgcct agaggctagg gaaaacgcac
     5581 acggacgatc atatctcaca gatatgtcac acattttcat ctcctactca cgcagagatc
     5641 tgaccttcgc acagaagatc gtagatgccc tcgcggcaca gaacgtggag atgtggatcg
     5701 attggaaaag cattccaaaa ggcgaggact gggagcagga gatttacagg ggaatagagg
     5761 cggccgatgc actcctgttc ctgataagcc ccgattccat tcggtctaaa atgtgcaaca
     5821 ctgaaatcat tcacgcgctg aagagtggga aacgcattct gcccgtcctt ctaaaaaaat
     5881 caaccacgag tgaattcctg gatgaaacgg cgaagaggga gatcgaaaga ctgaattggg
     5941 tattcatcca gaaagacatc gatgatttcg acggcgcaat tgaacaaatt cgaaagacga
     6001 ttcgaacgga ctacgattgg gtgaggtttc atacgaccct gctcaccaag gcacttgcct
     6061 gggaacgcgc tggcgaggat gccagccgac tactcagagg caaagagctg gcggaggcag
     6121 aaaaacaaat cgcggattca ggtccagcaa ccgaaccatt tctcacagat ttacaacgac
     6181 agttcctaac aaaaagcagg caatcagtta agcggcgaca agccctgcct tacagcgtgt
     6241 tggctcttct ggccgccaca atttttctgg gcggcaagtt cttttatttg atactcccaa
     6301 tctctagcgc atgccccgaa gtgggaagtg tttcgtttga aattccaagg gcaaaattga
     6361 ctgagtctac atatcaaacg cttctcgaag ccattgagaa cggtccatcg aaaacggcaa
     6421 ggcaaaattg cgaactcggc gctgaagaat tgatttacgt gacctttggc gattcttccg
     6481 ccggtgatca aatcgaactg tccgtgcggc tacctgaaat acctgcctat caattggatc
     6541 ttcttcagga aatccgggag tttggaccag aactcgttga acagtcggaa gcgatagctc
     6601 tgctgaacgc cttctctgct tattccgtgg gcgaatatca aacgtctatc gatcttctta
     6661 cagggtatga tagtctgagt tcactgactt tattggctca ggcacaatta tttagagatg
     6721 atctcggagc cagcagagag gcctatgatc tggcattgca gaagactcaa cctgacagcg
     6781 tttacacagg aaaattgaat atgggtgccg cacttgcctt gtggagacca gagagctact
     6841 acctgctctc tttcgaaggc aaaaaggagg attgtaaagc ggccgccata tattattcac
     6901 atgcgatgga tcagttcgaa gctgataaat tggcacacaa tgtcaggatc ctctacgcca
     6961 actactgttt taccgataat gataaaaccg tgcccgggct cgagcagtac gcggtgtgga
     7021 aggatgaagc accacaatca gaatcggatc ttcagtcgaa ggatgcgacg tatatcaatg
     7081 ccacggagca atacatcctt gctcttcgtg aacgaatgaa taaaggacct actcttcaat
     7141 ataggaacca cctaatttca gcgcaacctc ttatgctggc gcgggcagcg ctcagtgatt
     7201 tttactggaa agtagatgaa aactgcgcag aggctcgggt gagcagggat gaatttaggt
     7261 cttccatcta tagcgggata gaaaaatgga gcctcgaaag gctccttcaa agtcagccat
     7321 tcttttgcag gtcgtagcta gcctgcatta aaagtgggga aaatgccata cactgtgagc
     7381 accatggtta ctatcgtaga cggacatttc tccccagtcg cttgaactat gactaggaaa
     7441 atagatttcg tccgcgggtg agaagggagt attcaaaagg cggtcaacat ctgaatccct
     7501 aaataatgta agcaatttct cttgaattcg g
//
LOCUS       DASTJP010000568         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_192546, whole genome shotgun sequence.
ACCESSION   DASTJP010000568 DASTJP010000000
VERSION     DASTJP010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192546"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..896)
                     /locus_tag="VFQ23_16625"
     CDS             complement(306..896)
                     /locus_tag="VFQ23_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908275.1"
                     /translation="MKRFTLLFTLVGALAILALSVARAEASPEIAPASESIEEPSDAA
                     IPGAAASYKTVLGKSLRDKEFADFVASNNCSSTGQFQLCQDAGVALWLDADQKVNTVY
                     LYTVGANGFAAYAGALPQGLVSNDTMASVEEKFGQPFVPGVPQAGRKPGLPDRGGSPD
                     HIHYWAVYERFGLTIIYNTPFANDKNATIDAILISE"
     gene            1051..4179
                     /locus_tag="VFQ23_16630"
     CDS             1051..4179
                     /locus_tag="VFQ23_16630"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET9908276.1"
                     /translation="MPERLVLRFLGSPQLYLNEEPVTAERRKAVALLVYLAVEGGKHT
                     RDSLSALFWPDYDQSKAFTNLRHTLWEVQQAIGEGWLIADRETMELDLSADISLDVRD
                     FESVLVQSRAQQTPALRIPLLADAVKLYRNHFLTGFSLKDAPDFNEWAFAKSEDLRHK
                     LGSALTMLSDDHFAVGQADQAIPYARRLITLDPLNESSHRQLMKVYIQAGQHSAALKQ
                     YQACEQLLRKELGVDPQPETRELYKKIRKRDLKPLKAEQPTVTNTPQHNIPLQLSSFI
                     GREKEQKTIAKMVAGNRLVTLIGTGGIGKTRLSLKVGEAMLDKFEHGVWFVELASLND
                     GSLVPQAAAAVFGIIDRSETTSLEKLVGLLRAKASLLILDNCEHLLAACAQLADFLLK
                     NCPNLKILATSREGLGISGEALYRVPSLSVPKVEKNIEQLAESESIRLFTERARLTVS
                     DFCLKMENIPTIAEICRRLDGIPLAIELAAARVNILSEEQIAARLNESFSLLTSNSRT
                     KLARQQTLQASIDWSWNLLSDSERVLLRRLSVFAGGWTLEAAESVCSGDGIASEQVLE
                     IMTQLVMKSLIVAEQESGRERRYRLLEMIRQYAREKLIQSGEEENIHNQHLKYFLQLS
                     EKFEVEIIGHRQDEWFSRANDDRDNLRAALHWANRTNLEAGLNISARLLSHWEMSNLR
                     EGKQWLETFLNKSGSTDFPAARAAALLTYGWLLTWLQHFIPAHTATQESLNLFRSVSD
                     QRGEIDSLISLANIAQFTDAMESGTELLHQALRLSEALEDRWRQAESLGFLGWDRRDQ
                     KLAFQYWEKAIELYREVGDQHAMANLLGLLGLFKVLNGDIEVGEKYLNEATPLWESKN
                     RTNVWEHIKTTKSLILIARGDFDQAYEVLKESLVSAQKLGNRMSYFWQKVRMGYAALY
                     AGNLGEAHDSFVETTQSFHKDGYTVGAVYAIEGIASLLVMTGKPEKAAQLLGWADATR
                     EQIHDNRPLIEEANIYRTMSAIIAKIGPSAFEVAYDDGRSMTSEEAIRFALGEKTILE
                     QPIESVLKKSYD"
     gene            4172..>4652
                     /locus_tag="VFQ23_16635"
     CDS             4172..>4652
                     /locus_tag="VFQ23_16635"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9908277.1"
                     /translation="MTDISIRPIGESGHSHPSGTVTFLFTDIESSTHMWEQHPDEMKT
                     AYARHESILRQAVASHNGYAYKMIGDAFQVAFETAPQALAAALEAQRALHAEPWPETC
                     QMRVRMALHTGVTEERGDDYVGPALNRVARLLSAAYGGQVLLTQPVYDLVRDDLPFDI
                     "
BASE COUNT         1150 a         1137 c         1229 g         1136 t
ORIGIN      
        1 aagtcgccgg tggtcgagta gtcccgcatg agcgtggcag gacatacacc tgcaccgaac
       61 gcagctatgg tgtcgagacc acaaattttc agaaatgctt catttgcaag ttgctgttgg
      121 tctcgaaaca gcgtgagaac accgcttact cgaccaacgg attctttttg aaacagagtg
      181 tcaagtcact agtttctaat aaggcttttt tctatatcgc gatagagcgg gaacatgatt
      241 cgtggggcag ggttatatat ttcattgtac cctgccccac gtcacactct catatgctcc
      301 gtctgttatt cgctgatgag gatcgcatcg atggttgcgt ttttatcgtt tgcaaatggc
      361 gtattataga tgatggtcaa gccgaacctt tcgtacacag cccagtagtg gatgtgatct
      421 ggcgaaccgc ctctatcggg taagccaggc ttccggcctg cctgaggaac gcccgggaca
      481 aatggttggc cgaacttttc ctctacagat gccatggtgt cattggagac caaaccctgg
      541 ggtaaggcgc ctgcgtaggc agcaaagcca tttgcaccca ccgtatacag gtagactgta
      601 ttcactttct gatctgcatc cagccacaga gccacgccgg catcctggca taactggaac
      661 tgtccggtgc tggagcagtt attactggcg acaaaatcag caaactcttt gtctctcagc
      721 gatttgccga gcaccgtctt gtagcttgcg gctgctcctg gtatcgcggc atccgaaggc
      781 tcctcaatcg attcggaggc tggggcaatc tcagggctcg cttccgccct ggctacagac
      841 agtgccagga tcgccagcgc acctacaaga gtgaacaata gtgtgaaacg tttcatgata
      901 ttttctcctt tttgattagc tcgaatatgt ccacagtata gggatgccgc gtgtcgaaat
      961 cgtgtcgcgt cgcgtttcgg aacgcgtaac ctgatgcatt cctggcattc agagatataa
     1021 tcaaaaacaa gctgatgcaa aaggagaagt atgcctgaaa ggcttgttct acgctttctt
     1081 ggttcccctc aactttactt aaacgaagaa cctgtcactg ctgagcggcg caaagcagtg
     1141 gctttgctgg tttaccttgc cgtggagggt ggcaaacata ctcgcgactc tctgagtgcc
     1201 ttgttctggc ccgattatga tcagtccaag gcttttacca atctgcgtca tacgctgtgg
     1261 gaagtgcagc aggcgatcgg agaaggctgg ctcatagctg accgtgaaac gatggaattg
     1321 gatctttcag cagatatttc tctggacgtg cgcgactttg aatctgtact tgtgcaaagc
     1381 cgcgcccaac agactcctgc acttcgaatc ccgctgttgg ctgacgccgt taaactgtat
     1441 cgcaatcact ttctgaccgg gttcagtctt aaggacgcac cagacttcaa tgagtgggcg
     1501 tttgccaaat ctgaagacct gcgtcacaaa cttggcagcg cattgaccat gctttcggac
     1561 gatcactttg ctgtaggaca ggcagatcaa gccattccgt atgcacggcg actgatcacg
     1621 cttgatccat tgaacgagtc ttcacatcga caattgatga aggtttacat acaagccggt
     1681 cagcacagcg cggccttgaa acaatatcag gcttgcgagc agttattgcg caaggaactt
     1741 ggcgttgacc ctcagccaga gactcgcgaa ctttacaaga agattcgcaa gcgtgatctc
     1801 aaaccgctca aggccgagca gccaactgtc acaaatacgc ctcagcacaa tattcccttg
     1861 cagcttagtt ccttcatcgg gcgcgaaaag gaacagaaaa cgattgcaaa aatggttgca
     1921 gggaatcggc ttgttacgct gatcggtacc ggcggcatcg gcaaaacccg attgtcttta
     1981 aaagtcggcg aagcgatgct ggataaattc gaacacgggg tttggtttgt agaattggct
     2041 tctctcaacg atgggtcgct tgtgcctcag gccgcggccg ccgtgttcgg catcattgac
     2101 cgttctgaaa cgaccagcct ggaaaaactc gtcggtctgt tgcgtgcgaa agcttctctg
     2161 ctcattctcg ataattgtga acatttgctg gctgcctgcg cacaattggc agacttctta
     2221 ttaaagaact gtccaaactt gaaaatactt gctaccagtc gcgaagggtt ggggatatcg
     2281 ggagaagcac tgtatcgcgt cccatctctc tccgttccca aagtggaaaa aaatatcgag
     2341 cagctggctg aatcagaatc cattcgattg tttacagagc gtgcccgcct cacggtcagt
     2401 gatttctgcc tcaagatgga aaatattccg accatcgctg agatttgccg ccgcctggat
     2461 ggaatcccgc tggcgattga acttgcagcg gcgcgcgtga acatcctctc ggaggaacag
     2521 atcgcggcgc gactcaatga aagtttcagc ctgcttacca gcaacagccg tactaagctg
     2581 gcgcgccagc agacgttgca ggcttcgatc gattggagtt ggaatttgct ctctgattcg
     2641 gagcgcgttc tcctacgcag gctctccgtg ttcgcgggcg gctggactct cgaggcggcc
     2701 gagtcggttt gcagcggtga cgggatcgca tctgaacagg tgctggagat catgacacag
     2761 ctcgtgatga aatcgctgat tgtggcagaa caggagtcag gtcgcgaaag gcgatatcgt
     2821 cttctggaaa tgatccgtca atatgccaga gagaagctga ttcaatccgg cgaagaagaa
     2881 aacatccata accagcattt gaaatatttt cttcagttgt ccgaaaaatt cgaggtcgaa
     2941 attattggtc atcgacaaga tgaatggttt tcgcgagcaa atgatgatcg cgacaatttg
     3001 cgtgccgccc tgcactgggc gaatcgaacg aatctggaag ccgggttaaa tatctcagcc
     3061 agattgctgt ctcattggga aatgtccaat ttacgcgaag ggaaacaatg gctggaaacc
     3121 ttcttaaaca aatcagggtc aacggacttt cctgcggcca gagccgccgc attactcacc
     3181 tatggttggc tgttgacctg gcttcagcat tttattcctg cacataccgc aacccaggaa
     3241 agcctgaact tatttcgaag cgttagtgac cagcggggtg aaatcgactc gctgatttca
     3301 ctggcaaata ttgcacagtt caccgacgcg atggagtcag gaacagaatt gttgcatcag
     3361 gcactgaggt tatctgaagc tctggaggac cgctggcgac aagccgagtc tcttggattt
     3421 ttagggtggg atcgtcgtga tcaaaaactt gctttccagt attgggaaaa agcaattgag
     3481 ttatatcgtg aagtaggcga ccaacatgcc atggcaaatc tactgggttt gctgggtctg
     3541 tttaaggtat tgaatgggga cattgaagta ggagaaaaat acttgaatga ggcaactccg
     3601 ttgtgggagt ccaagaaccg gacgaatgtc tgggaacata tcaagaccac aaaaagcctg
     3661 atcctcatcg cccgtggtga ctttgaccag gcttatgagg ttctgaaaga gtctttggta
     3721 agcgctcaga aactggggaa tcgaatgtcc tatttctggc agaaagttcg catgggctat
     3781 gccgcgctct atgcgggcaa ccttggcgaa gcacacgact cttttgttga aaccacacag
     3841 agctttcata aggatggata cactgtcggc gccgtctatg caatagaggg cattgcgtct
     3901 ctgctggtga tgaccggtaa gcctgaaaag gctgctcaac ttctcggatg ggccgatgca
     3961 actcgcgaac aaatccacga taaccgtcct ctgatcgaag aagccaatat ctacagaacc
     4021 atgtctgcca ttatcgcgaa gatcggaccg tccgcgtttg aagttgccta tgatgatggg
     4081 cgttcgatga catctgagga agccatccgg tttgcgttgg gggagaaaac gattctggaa
     4141 caaccgattg aatcggtcct taagaagagc tatgactgat atttcaatac gcccgatcgg
     4201 ggaatcagga cattcccatc caagtgggac cgtaacattt cttttcaccg atatcgaaag
     4261 cagcacgcat atgtgggaac agcatcctga cgagatgaaa acggcatatg ctcgtcatga
     4321 aagcattctg cgtcaggctg tggcctcgca caatgggtat gcgtataaaa tgatcggaga
     4381 tgcgtttcaa gttgcctttg aaactgcacc tcaggcatta gccgccgcat tggaggcgca
     4441 gcgcgctctg catgcagaac catggcctga aacctgccaa atgcgcgtgc gcatggcttt
     4501 gcatacgggg gtaacagaag aacgaggtga cgattatgtg ggtccggcgc tcaatcgggt
     4561 cgcgcgtttg ttaagcgccg cctatggtgg gcaagtgctg ctgacacagc ctgtctatga
     4621 cctggtacgg gatgatttgc ctttcgacat cg
//
LOCUS       DASTJP010000569        15558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_466619, whole genome shotgun sequence.
ACCESSION   DASTJP010000569 DASTJP010000000
VERSION     DASTJP010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15558
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466619"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VFQ23_16640"
     CDS             complement(<1..1325)
                     /locus_tag="VFQ23_16640"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908278.1"
                     /translation="MKKKAPADTIPKSQFEELTLLYQLGILFASGKNLYETLLTLQNE
                     IVKLVQVDTMFVATYSENTDLVDYPIFFRRGESQPHASRKLGERPGLTGEVIYSKKTL
                     YLPDITADDVVKRHAPVGDSVLFLRTFLGIPLLVKEKVIGVLSAQSVKPDAYSNDQIR
                     LLENFAVQAALAIDKARLLDQLQQQLTERKQVEASLRQRESILEAVTFSAEQFLKASD
                     WREKINAILERLGKTIRASHAYLIEHYIESNGNEMAILRHEWTAQGFPGFLGSPAYAA
                     PYPVRGEEGTTNEFLMRGEVFAGTSSTFPVKDKARLNALGIKSLLEVPLFVNGMWWGT
                     IGFDDFEKERDWSPSEVDALRIAAGILSGTIQRQEAETAVQESERMYRQAIEAADAVP
                     YYLDYQKNHYPFIGEGIRAMTGYSADEIKPHVWGDLVLETIMLGDADGL"
     gene            complement(1322..5974)
                     /locus_tag="VFQ23_16645"
     CDS             complement(1322..5974)
                     /locus_tag="VFQ23_16645"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908279.1"
                     /translation="MFKQILHFLAPPVFAEDEEKTQTASLLNVITISTFAAASLYGLF
                     LPEDRILYAALAIIITLIVWLVMKGGYIQAASMFLVIGIFLVIAIAVITAGGVKAPEY
                     GAFIVPILFAGLLLGWQASVAIAICSILYGAIVLQAESFSILPETAAYPSTAFWILYS
                     LYFLLASNFLTLALRAVTRALQGTRKELTERIRIESDLYKREAILEAISKSAELLLKA
                     PEWRTEINTVLENLGQSINASHAYLYENVTNRNGEMVTSIEFAWTAPSGLRELDKSKY
                     KNVPLKEIGFASWFASLSTRRTYIGDSRSFSEAEADHLSSLGIKAMLDFPLIVDDQWW
                     GIIGFDDVVQEREWTNSEVDGMTIASNVLAAAIQRQKSDALLKAELHNHRQAEDVLLQ
                     FRAVLDESNDAIFMIDPETSRYIDFNRSAHERLGYSREELENLGAIDVAQHIPDIKVW
                     RKRVELIREKGELIFETVYRRKDGTTFPVEVSARMLEYRGELIMVAVTRDITERKLVE
                     AALRESEERFRQVFQSSPVAICITTLKEGRLLDGNSAYWELSGYDPQTSIGRDYMELR
                     MWDNPQDRIPFVEKLRQNRSFYNPDYIFMDTRGNQKSALAFYELIEIGHEECVLSMFH
                     DISVQKQTMDALQKSEARVRALLEAMPDMIFEISSDGVFLDSIHAQGVNTLLPPEMFL
                     GKNIREVMPPEVSSPAMFAIRRAVETGQLHAFEYILPMRNELRSYEARVMGVSSNKAI
                     AIIREITTRKQIEQEREKFINELEVKNAESETLRESLASIVGTLEFSEIIDRILAQIK
                     RVVPFDTASIWRVEGNQQIIIAGVDLPAEIRIPGTILTVSETNSAYPLLTGSLPYILN
                     NRVQDELTDFMNPRDSYVNSWLAVPLRTKGKIIGLIALDGKSRDQFTEHHAALAVAFA
                     NQVAIALENASLFYELHAELEARKDLIAELEAKNAEAETLRESTVIVAETLEKNEAIE
                     RILEQLERVVPYDSASVQLLHGNSLEIVGGRELPDLNHVGLRFVVDENEPSYPILQGN
                     AQYVLYHDVQLYLPKFREPTHNRIRAWLAVPLKVKGNLVGIIALDGYRAGQFSERHAQ
                     LAATYANQVAIALENARLFSDLQGELTERKQIEINLRQRESILEAVSDAANLFLKTSD
                     WKAEINSVLERLGKIVNASHTYLFQNHQLENGEIVASIRYEWTAPSFVSDLDNPSYSN
                     APVKEKDFEEWFDVMAKGTPYIGDARHLSQLDMDFLLERGIKALLDMPIFVNGEWWGT
                     IGFDDMQQAREWSNAEVDTLVTAANVLGAAIQRQQAESKLQDELMYRKKLIAELESKN
                     AELERFTYTVSHDLKSPLFTIRGFVGYLEKDALSGNQERLRSDIQRIHDATEKMQRLL
                     NELLELSRIGRLMNELQPVSIDELAREVLELVQGRIMERGITVHIESNLPTVFGDRPR
                     LFEVLQNLVDNAAKFMGDQKDPRIEIGQLGETEGMPVLYVKDNGVGIPPEHHERIFGL
                     FNKLDVKSDGTGIGLSIVKRIIEVHGGKIWVESHQGIGTTFFFTLPVSAADKETTL"
     gene            complement(6105..6869)
                     /locus_tag="VFQ23_16650"
     CDS             complement(6105..6869)
                     /locus_tag="VFQ23_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908280.1"
                     /translation="MKRRIWIATVMVVLGLSSCNLQGVQPTETALPSPTLVIPITSAS
                     TPTPIPIDTLLAIDLETPTVVPTSTSSSVLASPIDQPVNCRFGPGTQYGIAGALNPGR
                     QAEMIGKNEDSSWWYVKNPSDPSTLCWLSAGVTSTVGNVDSLPVVEPPEIMVTSVNVS
                     VDPPGINVACDAFPQSVILSAFISTNGPSIVVWRWESSNGRTSAENNLLFEEGGTKMV
                     QDYFQVDSANDYAIQVRTIVPNVVIGQADIRVTCTP"
     gene            7237..7815
                     /locus_tag="VFQ23_16655"
     CDS             7237..7815
                     /locus_tag="VFQ23_16655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9908281.1"
                     /translation="MVTGLNVSETELVNRAQNGDRNAFSELVRNHAQGVLNVIYRMCG
                     DAHIAEDAAQETFIQAWLHLSTYRPQTSLRNWLYRIAVNAATDLLRKEKHILPNSLED
                     LQLTDGQPGPESLTSQKERIVLVQKAVLALPEASRAVVVLREYEGLSYQEISSTLEIP
                     IGTVMSRLNYARKLLKEKLEQSFVLPVEVDYA"
     gene            7808..8416
                     /locus_tag="VFQ23_16660"
     CDS             7808..8416
                     /locus_tag="VFQ23_16660"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9908282.1"
                     /translation="MPDHVTEWLNAYMDDELHGNRLRQVKEHLAECEACQAELSSLRD
                     LSGLLRKVPTPDFTSPERFATQVNLLLPQKPVPSPKNSLLEIGWWMIPVGLLAVWAFV
                     GTASIVSSLVSAASNIGLVDSANTLLISGTSDANHWTSTLRQLGLLEGNSLQWSEMTE
                     GYSRNVIPQFVWQASIALLYLTWIAIWWARQTRQGYGQLLKS"
     gene            8474..9607
                     /locus_tag="VFQ23_16665"
     CDS             8474..9607
                     /locus_tag="VFQ23_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908283.1"
                     /translation="MTIDPIKIIKRAWYILWNYRALWVFGLVLALVAGGSSAGQGSNN
                     SYQYQQNPQENPEVSPQSMQEAFRDFQREMNKLFEQGISDINVSGQDLTTFLWIVGAF
                     VLVMLVLGIVMAIARYVSETAVIRMVDEYESTENKMTVRQGIRIGWSNTSWRLFLINL
                     IVNLPAIALALVLLISGIGVFFAVVNGSADFAAFSVVSTVVLIFLTIFVVVILTILLH
                     LLRNFFWRICVLENASVRESFQRGFAMVLENWKNVGLMWLVMIGLGIVWTVASIILFI
                     ITIPVVVVTAILAVLVVALPFLLLVGAFSLIVGGILPWIAAGLFVAPLFFTLAFSPWV
                     LLGSWQAVYTSTVWTLTYREIKALPELAAPVLTTPPPVEPQTS"
     gene            complement(9647..10894)
                     /locus_tag="VFQ23_16670"
     CDS             complement(9647..10894)
                     /locus_tag="VFQ23_16670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET9908284.1"
                     /translation="MEHELTLTTFTYGGDVMGRLDDGRAVFVPFGLPGERVRVRLTEE
                     KKNFARGEIVEILEPSKERIIPRCKHFGECGGCHYQHLPYEKQLQIKTDILRDQLQRI
                     GKIENPPVNPIIASPNPWNYRNHVQFHLTENGKLGYFRSTDKINLPEKILPITECHLP
                     EPPINDLWPQLEFEPGTDVERVSLRTGKDDDLMVVLESESSEPPDLEIEAGISVSHLY
                     EENSIVIAGTDHVIMRILDRDFRVSASSFFQVNTVMAEKMVSHLIANLPINTTATLID
                     IYCGVGLFSAFFASRCKSVIGIEASPSACEDFAYNLDEFDNVELYEGSAEDVMPAFVG
                     RIDNPTYIIVDPPRAGLDKHVVDGILKLHPHMIAYVSCDPSTLARDAARLVNGGYKLR
                     DVTPFDLFPQTYHIESISIFEPK"
     gene            complement(10941..12008)
                     /locus_tag="VFQ23_16675"
     CDS             complement(10941..12008)
                     /locus_tag="VFQ23_16675"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET9908285.1"
                     /translation="MSRMFVPGPVDVADEVLQAQAAPMLPHRSKEFETIFRRASEKSQ
                     QLFYTRHRVFLTASSGTGLQEAAIRNFVHKRVLSVVNGAFASRWFEVAVYNGKETDEL
                     AFEWDTPASPDVVAEAVKQGGYEAVTVVHNETSTGLQNPVKDISEAVRAVAPDTLIFV
                     DAVSSLGGAKIEMDAWGIDMLLTSSQKCLALPPGLALGAVSDRAMKKAESVENRGWYF
                     DLVRMEKHRNKDSSPATPAMSLIYALDFQLDRILAEGLDNRFARHSAMAKRVQEWADA
                     HDLSMYAPEGYRSQTVTTIKNDRGFSVSELNTFLKQREMRIANGYGQLKDKTFRIAHM
                     GEIQMDDIEKLLEAMEEFLQK"
     gene            complement(12055..12234)
                     /locus_tag="VFQ23_16680"
     CDS             complement(12055..12234)
                     /locus_tag="VFQ23_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908286.1"
                     /translation="MAKKSKRPATKVTTVSVTPTTTRTEFNPDYTHVRKDLTRIGTLA
                     GFFIVLLVALSFILR"
     gene            complement(12243..13145)
                     /locus_tag="VFQ23_16685"
     CDS             complement(12243..13145)
                     /locus_tag="VFQ23_16685"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HET9908287.1"
                     /translation="MPRRKAPEDMSVEELRRLLIEKRRGARRERLEHFKRTGRVVDVE
                     PDALDSSAPVVDTLEGTSDQPQVPVSRRRRVMDRVLLVVEVLAVVGLVGVLISGFGLL
                     RDLNDEVAAALIQETFTPTPLVMAVVLPSGHTPPDAQGNTRPNDAEIPSHLQPMVQSL
                     ANIPVPTAAPDQAIRIQIPALEIDAPIVQGDGWEQLKKGVGQNIGSANPGQNGNVVLS
                     AHNDVYGEIFRYLDKLVPGDQVVVYTQQRQYVYVVDRTAIVEPTAVEVMASTGNPTVT
                     LISCYPYLVNKQRIVVFARLQNQN"
     gene            13226..13302
                     /locus_tag="VFQ23_16690"
     tRNA            13226..13302
                     /locus_tag="VFQ23_16690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13260..13262,aa:Val,seq:gac)
     gene            complement(13334..13804)
                     /locus_tag="VFQ23_16695"
     CDS             complement(13334..13804)
                     /locus_tag="VFQ23_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908288.1"
                     /translation="MKTRKATARESLLLSSLCVDVQRLHAEHHPEIFKMPHDDDFAVS
                     FFQEILTDAVTHIFIAEEKQRAIGYIFCKLFERPEGPFTHANRFLQLEHISVRPEARR
                     NGVGMALMRRAVKLAGKLGAKKILLSSWDFNTPAHAFFERHGFAKIEHRFWRAL"
     gene            14371..>15558
                     /gene="thrS"
                     /locus_tag="VFQ23_16700"
     CDS             14371..>15558
                     /gene="thrS"
                     /locus_tag="VFQ23_16700"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET9908289.1"
                     /translation="MSQQVQEKYEESQLYKIRHSAAHVMAQAVMEMFPDGKITIGPPV
                     ENGFYYDFDLPRNLTPEDLAAIEKRMRQVVQGKHEFMKKVVSAEEAREIFKDQPYKIE
                     LIDGLEKGGLDEYGNPLKEKPEISIYQHDTFVDLCRGPHVANTKEIKQDAFKLMSIAG
                     AYWRGDEKNKQLQRVYGTAWENKKQLEEYLHNLEEAKKRDHRKLGRELEIYMFDDEVG
                     PGLPLFLPNGGIMIEELEKLAKEMEDKAGYVRVRTPNLTKEDLFIRSGHLPYYAESMY
                     PPMELEGVKYYVKPMNCPMHHKIFASKGRSYRDLPIRLAEYGTVYRYEKSGELFGLMR
                     VRSLQMNDAHMYVTEDQFEQEFLGVVDLYLKYFELFGIEKYVMRLSLHSKAGLGKKYV
                     DNER"
BASE COUNT         3946 a         3926 c         3714 g         3972 t
ORIGIN      
        1 ctcaacccat ctgcatcgcc cagcatgatc gtctctaata caagatcccc ccacacgtga
       61 ggctttattt catccgcgct ataaccggtc atggcacgga tcccttcccc tataaaaggg
      121 taatgatttt tttgataatc aagataatac ggtacggcat ctgccgcctc gatagcctgg
      181 cgatacatgc gctcagactc ctggacagct gtttcagctt cctggcgctg gatcgtcccg
      241 ctgaggatcc cggctgcgat cctcaacgca tcgacttcag aaggcgacca gtcccgttcc
      301 ttttcaaagt catcgaatcc aatcgtaccc caccacatac cattaacgaa caaaggtact
      361 tccagcagag atttgattcc gagcgcattc aagcgcgcct tatctttcac agggaaagtt
      421 gaagaagtgc cggcaaatac ctcgccacgc atcagaaatt cattcgtggt tccctcttca
      481 ccgcgtacag gatatggtgc ggcatacgcc gggctgccta aaaatccagg aaaaccctgt
      541 gcagtccatt catggcgtaa gattgccatc tcgttaccat tcgactcgat gtaatgctct
      601 ataagatagg catgacttgc ccggatggtt tttcccaacc gttccagaat ggcattgatt
      661 ttctcgcgcc aatcggatgc tttgagaaat tgttctgctg agaaggtaac ggcttcaaga
      721 atggactcac gctgccgcaa gctggcttcc acttgtttcc tttcagtcaa ctgctgttga
      781 agctgatcga gcaatctagc cttatcaatc gcaagcgcag cctgaaccgc aaaattttcc
      841 agcagccgaa tctggtcgtt ggaataggca tcaggtttta cgctctgcgc cgagagaact
      901 cctattacct tttccttaac caacaacggt atacccagga aagtacgcaa aaataagacg
      961 ctatcaccga cgggtgcatg tcgtttcaca acatcatcgg cggttatatc tggcaggtaa
     1021 agagttttct tgctgtatat gacttccccg gttaatccgg gtctttcgcc aagtttacga
     1081 gaagcatgtg gttgagactc gcctctccga aagaaaattg ggtaatccac aagatctgta
     1141 ttttcgctat aggtcgcaac gaacattgta tccacttgaa caagcttgac aatttcattt
     1201 tgaagtgtaa gtaaagtctc atacagattc ttaccggaag caaacaggat gcccagttga
     1261 tacaataaag ttagttcttc aaactggctc ttcggtatcg tatcggcggg cgccttcttt
     1321 ttcatagggt tgtttcctta tccgctgcgc tgactggaag ggtgaaaaag aatgtagttc
     1381 caattccctg gtggctctcc acccagatct tccctccgtg cacctcaatg atcctcttca
     1441 caatagaaag tccgatgcct gtcccatcgc tttttacatc caatttgtta aagagtccaa
     1501 aaatgcgttc atgatgttca ggtgggatcc ccactccatt gtctttgacg tacagaaccg
     1561 gcatcccctc tgtttcgccc agctgaccaa tctctatgcg tgggtctttt tgatctccca
     1621 tgaacttggc cgcattgtcc accaggttct gcaaaacttc gaataaccgt ggcctgtcac
     1681 caaacacggt gggcaagttg ctttcaatgt gcactgttat tccgcgctcc ataattcgcc
     1741 cctgcacgag ttcaagcacc tcacgcgcca attcatcgat tgaaacaggc tgtagttcat
     1801 tcatcagacg cccgatgcgc gaaagttcga gcagttcatt caaaagtctc tgcatctttt
     1861 cagtcgcgtc atgaatacgt tggatatctg atctcaagcg ttcctgattc cccgaaaggg
     1921 catccttctc cagataaccg acaaaaccgc ggatcgtaaa tagcggtgac tttagatcat
     1981 gagagactgt ataggtaaat cgctcgagct ccgcgttttt tgattccagc tcggcgatta
     2041 atttcttgcg gtacatcagt tcatcctgta atttggattc agcctgttgg cgctgaatcg
     2101 ctgcacccag cacatttgcc gcagtgacga gcgtatccac ctctgcattc gaccactcgc
     2161 gcgcctgttg catatcatca aaaccaattg tcccccacca ctcgccattc acaaaaatag
     2221 gcatgtccag caacgctttg atgccgcgct ccagaagaaa atccatatcc aattggctta
     2281 agtgcctggc atcaccaata tagggtgtgc cttttgccat gacatcaaac cactcttcaa
     2341 aatccttttc cttgacgggc gcatttgaat agctggggtt atcaagatcg ctcacaaatg
     2401 aaggcgctgt ccattcataa cgaatcgatg caacaatttc cccattctcc aattgatgat
     2461 tctgaaataa atatgtgtga ctggcgttga cgatcttccc cagtcgttcc agaacagagt
     2521 tgatttctgc cttccagtca gaagttttca aaaacaggtt cgccgcgtct gacacagctt
     2581 ctaaaatgga ttctcgttga cgcagattga tctcaatttg tttgcgttct gtcaattcac
     2641 cctggagatc cgaaaaaaga cgagcatttt ccagagcaat agcaacctga tttgcatagg
     2701 tagctgccaa ttgtgcgtgt cgctctgaaa actgaccagc tcgataccca tccagagcaa
     2761 tgatgccaac aaggtttccc ttcactttca gcggaacagc cagccaggca cgaatccgat
     2821 tatgggtggg ttccctgaat tttggtaaat aaagctgaac atcatgatat agaacatatt
     2881 gagcattccc ctgcaagatc gggtaggaag gctcattttc gtcaacaaca aatctgagcc
     2941 caacgtgatt gagatcagga agctcacgtc caccgacgat ttcaagagag tttccatgta
     3001 acaactggac agaggcgctg tcatagggaa ccacgcgttc cagctgttca aggatgcgct
     3061 cgatcgcctc atttttttca agcgtctcgg ccacgatgac tgtactttcg cgcagggtct
     3121 ccgcttcagc gttttttgcc tccaattctg caatcaggtc ttttctcgct tccagttccg
     3181 cgtgcagctc atagaacaaa ctggcattct ccagggcaat cgctacctgg tttgcgaacg
     3241 ccacagccaa cgcggcatga tgctccgtga attgatccct gcttttccca tccagagcaa
     3301 tcaaaccgat aatctttccc tttgttctta aggggaccgc cagccaggaa ttgacatagc
     3361 tatcgcgcgg attcataaaa tctgtcagtt catcctgcac cctgttgttc aaaatgtagg
     3421 gaagcgatcc cgtaagcaga ggatatgcag agttcgtttc actaaccgta agtattgttc
     3481 cgggaatgcg tatttcagcg ggcagatcta cacccgcgat aatgatttgt tgattgcctt
     3541 ccactctcca gatagacgct gtatcaaatg gcaccaccct tttgatttga gcaagtattc
     3601 gatcaatgat ttccgaaaat tccaatgtac caacgatgct ggcaaggctc tcgcgcagag
     3661 tctctgactc tgcgttcttc acctccaatt cattgatgaa cttctcgcgt tcctgctcga
     3721 tctgtttgcg cgtcgtgatc tctcggatga tagcaatcgc tttattactc gaaactccca
     3781 tgacccgcgc ttcgtaactg cgaagttcat tccgcatcgg caaaatatat tcaaaagcat
     3841 gtagttgtcc tgtttcaacc gcgcgacgaa tggcgaacat cgccggagag gatacttctg
     3901 gaggcattac ctcgcgtatg tttttcccga gaaacatctc cggaggcaga agcgtgttga
     3961 cgccctgagc atggatcgaa tccagaaaga cgccgtcact tgaaatttca aagatcatat
     4021 cgggcatcgc ttcaagcaag gcacgcacgc gtgcttcgct cttctgcaat gcatccatgg
     4081 tttgcttttg aacgctgata tcatggaaca tggaaagaac gcattcttcg tgaccaatct
     4141 cgatcagctc ataaaaggca agagcagact tttgatttcc acgggtgtcc ataaaaatat
     4201 aatccgggtt atagaacgaa cggttctgtc ttaatttttc gacaaagggg atacgatcct
     4261 gtgggttatc ccacattcta agttccatat aatcccgccc gatcgaggtc tgaggatcgt
     4321 accctgataa ttcccaatag gcagagtttc cgtctagcaa gcgaccttct tttagggtgg
     4381 tgatacaaat cgcaacaggg ctggattgaa agacttgcct aaacctttcc tcactttcac
     4441 gtaacgcagc ctccaccaat tttcgttccg tgatatcacg cgtaactgca accataatca
     4501 attcccctcg atactctaac attcgggcgc tgacctccac cgggaatgtc gttccatcct
     4561 ttcggcggta gacggtttcg aaaatcaact ctcccttttc gcggataagt tccacacgtt
     4621 ttcgccacac ctttatgtcc gggatatgtt gtgcgacgtc aattgcaccc aggttctcta
     4681 attcctcgcg gctgtaaccg agacgctcgt gtgcacttct gttgaaatca atataacgac
     4741 ttgtttccgg gtcaatcatg aaaatcgcat cattggattc atcgagcacg gccctgaatt
     4801 ggagcaggac atcctcggct tgcctatgat tatgcaactc tgctttcaat aacgcgtccg
     4861 atttttggcg ctgaatagct gccgcaagta cattggatgc aatcgtcatg ccgtccactt
     4921 ccgaattggt ccattcgcgt tcctgaacga catcatcaaa accaataatt ccccaccatt
     4981 gatcatcgac aatcaaggga aaatccaaca tggccttgat acctaatgag gataaatgat
     5041 ccgcttcagc ttcactgaag gagcgactgt caccaatgta agttcgcctg gtactgagag
     5101 aggcaaacca gcttgcaaac ccaatctctt taagggggac gtttttgtac ttggatttat
     5161 cgagctccct taagccggat ggcgctgtcc aggcaaactc gatggatgtc accatttctc
     5221 catttcggtt cgttacgttc tcatataaat aagcgtgact ggcattaatg gactgtccga
     5281 ggttttcgag tacggtatta atttctgttc tccactccgg tgcctttaac aacagttcgg
     5341 cacttttgga gattgcttca agaattgctt cgcgcttgta taaatctgat tcgatacgta
     5401 tgcgttcagt caattctttt cgcgttcctt gcagtgcccg ggtgactgcc cgaagggcga
     5461 gagtaagaaa gttacttgcc agaaggaagt aaaggctata caggatccag aatgcagtcg
     5521 agggatatgc agctgtttca ggcagtatgg agaatgattc agcctgcagc actatggccc
     5581 catataaaat gctgcagatg gcgatcgcta ctgaggcttg ccaacccaac aataagccag
     5641 caaaaagaat tggcacaata aaagcaccgt attcgggtgc ttttactccg cctgcagtaa
     5701 ttacggcaat ggcaataacg agaaaaatcc cgatcaccaa aaacatggaa gctgcctgga
     5761 tatagccacc cttcattacc aaccagacaa ttaaggtgat gatgatggca agggcagcgt
     5821 agagaattcg atcttcaggc aaaaaaagac catacaaaga cgcagccgca aatgtactga
     5881 tagtgattac attgagcaga gaggcggttt gagtcttttc ttcatcctcc gcgaaaacag
     5941 gcggagccag gaaatgtagg atttgcttga acatgttagc tacttcaact tggattatat
     6001 agcatgtcca atccgcagaa cctaacaaag tttgaaggtt ggcgttccat ccacatttca
     6061 ttcacccgaa gacgaacgaa actattgagg tgaaattata taaattatgg cgtacaagtt
     6121 actctaatat cagcctgccc aataacgacg ttgggaacga tcgtccgcac ctgtatcgca
     6181 taatcgttcg cgctatccac ctggaagtaa tcctgaacca tttttgtacc tccctcttca
     6241 aacaagagat tattttcagc cgaggttctg ccattgctcg actcccagcg ccaaaccaca
     6301 atggatggtc cattggtgct gataaacgcg ctgaggatga cgctctgggg aaatgcgtca
     6361 caggctacgt tgattcccgg cgggtcaaca ctgacattga cacttgtgac catgatctca
     6421 ggcggttcca ccactggcaa cgagtccacg ttgccgacgg tgctggtcac tccagcagaa
     6481 agccagcaca gcgtagaagg gtcactcgga ttcttgacat accaccacga tgaatcctca
     6541 tttttaccga tcatttccgc ctgtctaccc gggtttaagg ctcccgcaat cccatattga
     6601 gtaccaggtc caaatcgaca atttaccggc tgatcgattg gagatgcaag gaccgagctc
     6661 gacgtagaag tcggcaccac agtcggagtt tcaagatcga tggctaacaa cgtgtctata
     6721 gggatcggcg tgggggtgga ggcgcttgtt atcgggatga caagcgtcgg cgatgggaga
     6781 gctgtttctg tcggctgaac cccttgcaaa ttacaggacg acagtcccag cacaaccatg
     6841 acagtcgcaa tccaaattcg gcgtttcatt actcctcctc gtaaagtaaa atcctactcc
     6901 catgctttag ttaaggcaat aggacgtagg atacagaggg aatgagttta ttcagagcag
     6961 gtagactata cctcttatag ttccatttcc cgactccgtt ttgcattgtc cgcgaaccaa
     7021 aaataatctc cttctccaag cctggtcagg cacgacaaat ccccgggtgg aaccacatta
     7081 ggcttgaaat acacctctaa ttttgccttc tcaggcactc catatctata aatacaatcg
     7141 accttcaagt cttctaatgg gaaacctctt ccgcttttcg aataaaagag ggcggattta
     7201 cgttaagata tcgaagcaca aaaggaaaat gcagcattgg taacaggttt gaacgtcagt
     7261 gagaccgaat tagtcaacag ggcacagaat ggtgaccgaa atgcattcag cgaattggtt
     7321 cgcaaccatg cgcaaggtgt gttgaacgtc atctatcgca tgtgtggtga tgctcatatt
     7381 gctgaggatg ccgcccagga gacattcatc caggcctggc tgcacctttc cacctatcgg
     7441 ccgcagacgt cgctacgcaa ctggttgtat cgcatcgcag tcaatgccgc cacagacctg
     7501 ttgcgcaagg agaaacatat cctgcccaat tccctggaag acctgcaatt gaccgatgga
     7561 caacccggac ctgaatccct cacctctcaa aaggagcgga tcgtactggt gcaaaaagca
     7621 gttcttgcac taccggaggc cagtcgggcc gtcgtggtgc ttagagaata cgaaggtctt
     7681 tcgtatcagg agatctcgtc cactctcgag attccgattg gaaccgttat gtcacgattg
     7741 aattatgccc gcaaattatt gaaagaaaaa cttgagcaaa gctttgtatt accggtggag
     7801 gttgattatg cctgaccatg taacggaatg gttgaatgcc tatatggatg atgaattaca
     7861 cggcaatcga cttcgtcaag tgaaagaaca tctggcagaa tgtgaagcct gccaggctga
     7921 gttgagctct ttacgagact tgtcgggtct tctccgtaag gtccccacgc cagactttac
     7981 ctcccccgag cgcttcgcca ctcaggtcaa tttgctcctg ccacaaaagc ctgttccatc
     8041 cccaaaaaac agcctcttgg aaattggctg gtggatgatc ccggttgggt tgctggctgt
     8101 gtgggctttt gtcggcactg catccattgt tagcagcctg gtttccgcag cgagcaacat
     8161 tggattagta gacagtgcaa ataccttatt gatctcgggc acgtccgatg cgaatcattg
     8221 gacgtccact ctgcggcagc ttggtttact ggaaggcaat agtttgcaat ggtcagaaat
     8281 gacggagggg tactcaagga atgtaatacc gcaattcgtc tggcaggctt cgatcgcact
     8341 gttgtacctg acctggattg cgatctggtg ggcgcgccaa acgcgtcagg gatatggcca
     8401 actgctaaaa agctgaggca ggcccacatc tggaagaaag cgattaagaa agttgataaa
     8461 caaggagaat agaatgacaa ttgatcccat caaaattata aagcgtgcct ggtatattct
     8521 ttggaattat agggcgctat gggtctttgg tttggtattg gcactggttg ccggtggtag
     8581 ttccgccggt cagggttcga acaatagcta ccagtatcag cagaatccgc aagaaaaccc
     8641 agaggtatcc ccgcaaagta tgcaggaggc gttcagagat ttccagcgcg agatgaataa
     8701 actcttcgag cagggaatct cggatatcaa tgtctcaggg caagacctga ccacctttct
     8761 ttggatcgtt ggtgccttcg tcctggtgat gctggttttg ggaatcgtca tggcaattgc
     8821 acgatacgtt tctgaaacgg cggtcatccg catggtagat gaatacgaaa gtacagaaaa
     8881 taagatgacc gtacgtcagg ggatacgcat cgggtggtcg aatacgtcgt ggcgattatt
     8941 cctgatcaac ctgatcgtca atctgcctgc gatcgctctg gcgctggtgc tgcttatctc
     9001 aggaataggc gttttcttcg ctgtggtcaa tggcagtgcc gacttcgccg cattcagcgt
     9061 agtctccaca gtggtgctta tcttccttac catattcgtg gttgtgatcc tgaccattct
     9121 gctgcatttg ttgcgcaatt tcttctggcg aatttgtgtg ctggagaatg caagtgtacg
     9181 cgaatcgttt caacgcgggt ttgccatggt actcgaaaac tggaagaacg ttggcttgat
     9241 gtggctcgta atgatcggac tgggaatcgt ttggacagtt gcgtcaatca tcctgttcat
     9301 catcactatc cctgtcgtgg tcgtgacagc catcctcgct gtgctggtgg tggcgctccc
     9361 gtttctgctg ctggtcggcg ccttcagcct cattgtcggt ggcatcctcc cgtggattgc
     9421 ggccgggctc ttcgttgcac cgttgttctt taccctggcc ttctcgccgt gggtgctgtt
     9481 gggcagctgg caggcagtgt acacgtccac cgtgtggacc ttgacctatc gcgagatcaa
     9541 agctctacct gagctggcag ccccagtgct gacaacaccg ccgcccgttg aacctcagac
     9601 aagttagttg aaacgcaaaa tccctttcca gcttggaaag ggatttttat tttggctcaa
     9661 agatggaaat gctttcgatg tgataggtct gcggaaaaag atcgaacggt gtcacgtctc
     9721 tcaatttata gccgccattg acaagccggg ctgcgtcccg cgctagcgta gatggatcac
     9781 atgagacata ggcgatcatg tgcggatgca atttcagaat tccatccacc acgtgcttat
     9841 caagacctgc ccgtggcggg tcaacgataa tgtaggtcgg attgtcaatc cgacctacga
     9901 atgcaggcat cacatcttca gccgagcctt cgtaaagttc cacgttatcg aattcatcaa
     9961 gattgtatgc gaaatcttca caggccgatg gtgatgcttc gatcccaatt acagatttgc
    10021 atctggatgc gaagaacgcg ctgaataaac ccaccccaca ataaatatcg atcagagtag
    10081 cggtggtgtt tatgggcaaa ttggcaatca agtggctgac catcttctct gccatgactg
    10141 tattgacctg gaagaacgaa gatgcagaga ctcggaagtc acgatcgaga atccgcatga
    10201 tgacatggtc agttcctgca atcacaatcg aattctcttc atataaatgg ctaaccgata
    10261 tccctgcttc gatttccagg tcgggcggct cggatgattc agattccagc accaccatta
    10321 aatcatcgtc tttgcccgta cgaagtgaaa cgcgttcaac gtccgtgccc ggttcgaatt
    10381 cgagctgggg ccacaaatcg ttgatgggcg gttcagggag atgacattcg gtaatcggaa
    10441 gtatcttctc gggcaagttt attttgtcgg tcgaacgaaa atagccaagt ttgccattct
    10501 ctgtgagatg gaattgcaca tgattgcggt aattccacgg gttgggagag gcgattatag
    10561 gattgaccgg agggttttcg atcttgccaa tacgctgcaa ttgatcgcgt aagatatctg
    10621 ttttgatttg caattgtttt tcataaggta aatgttgata gtgacatcct ccacattccc
    10681 caaaatgttt gcacctggga ataatacgtt ctttcgaggg ttcaaggatt tcaacaattt
    10741 ccccgcgtgc aaaatttttc ttttcctctg tcaacctcac ccgtactctt tcccccggca
    10801 gcccaaacgg gacgaagact gcgcgtccat cgtcaagacg acccatgaca tcgccaccat
    10861 aggtgaaagt tgtgagagta agttcgtgtt ccataaaaat aaaaggcgac cacgccgtcg
    10921 gcgggtcgcc cgtgcaggcg ttatttctgg agaaattctt ccatggcctc gagcaatttc
    10981 tcgatatcgt ccatctgtat ttcgcccatg tgcgcaatac ggaaggtttt atctttgagt
    11041 tgaccatagc cattggcaat acgcatttcg cgttgcttca ggaacgtgtt caattcagac
    11101 acactaaagc ctcgatcatt ttttatcgta gtcacagttt gcgaacgata gccttccggc
    11161 gcgtacatgg ataggtcatg ggcatctgcc cactcctgaa cacgctttgc catcgcagag
    11221 tgacgagcga agcgattgtc caagccctcg gcaagaatac gatcaagctg aaaatcaagt
    11281 gcatagatca gagacatagc cggcgtggca ggagaggagt ccttattgcg atgcttttcc
    11341 atgcggacaa gatcaaaata ccagccgcgg ttttcgacag attctgcctt cttcatggcg
    11401 cgatctgaga ctgcccccag agcaagcccg ggtgggagcg ccaggcattt ctgtgatgag
    11461 gtaagcaaca tatcaattcc ccaggcgtcc atctcgatct ttgctccgcc aagggaggac
    11521 actgcatcca cgaaaatgag cgtatcgggt gccaccgcgc gaactgcctc cgagatatct
    11581 tttactggat tctgcaagcc ggtggaggtc tcgttgtgaa caaccgtcac ggcttcatac
    11641 cctccttgct taacggcctc cgctacgacg tcgggtgagg ccggtgtgtc ccattcaaag
    11701 gcaagttcat cggtttcttt gccgttatag acagcgacct cgaaccagcg actggcaaag
    11761 gctccgttga ccacagaaag cacgcgttta tgtacaaagt tgcgaatcgc agcttcctgt
    11821 aagcctgtcc cggaggaagc cgttagaaac acacgatgtc tcgtataaaa gagctgttgt
    11881 gatttttcag aggcgcggcg aaagatcgtt tcaaattcct tgcttcggtg tggaagcata
    11941 ggcgcggctt gtgcctgtaa aacttcatct gcaacatcca ccggaccggg gacaaacatg
    12001 cgcgacataa tatctcccat gtagaaagac acagcgaggc tgtgtctttc tgtgttatct
    12061 caaaatgaaa gagagcgcaa ccaataggac gatgaaaaat cccgcgagcg tgccaatccg
    12121 ggtcagatct tttctgacat gagtgtaatc cggattgaac tcggttcggg ttgtcgtcgg
    12181 tgtaacactt actgtcgtta ctttggtcgc cggtcttttg cttttctttg ccatttgtac
    12241 tcctagttct gattttgtaa tcgcgcaaaa acaacaatgc gctgtttgtt cacaagatac
    12301 gggtaacagg aaattaatgt cacggttggg tttcctgtag aggccatgac ttccactgct
    12361 gtaggctcaa caatagctgt tcggtccact acgtaaacat actgtctttg ttgcgtgtac
    12421 acgacaacct ggtcgccggg aaccagctta tctaaatatc gaaagatctc tccgtacaca
    12481 tcgttatgtg cagatagaac tacattcccg ttttgcccgg ggtttgcgga accaatattc
    12541 tgcccaacac cttttttgag ttgttcccat ccatcccctt gcacaattgg agcatcaatc
    12601 tcaagcgccg ggatttgaat gcggatcgcc tgatcgggtg cggccgttgg aaccggaata
    12661 ttcgccagtg actgcaccat cggttgcaaa tgagacggga tctctgcatc attcggacgc
    12721 gtatttccct gggcatccgg cggagtgtga cctgatggaa gcaccacagc catcaccaag
    12781 ggagtgggcg tgaatgtttc ctgaatcagc gccgcagcca cttcgtcgtt gagatcgcgt
    12841 aaaagcccaa acccgctgat gaggacgcca accagcccga ccactgccag gacttccact
    12901 accaggagga ctcgatccat gacgcgcctg cgcctcgaaa ccgggacctg aggctggtcg
    12961 ctggttccct cgagcgtatc aaccactggc gcggatgagt ctaaagcatc tggttctaca
    13021 tcaacgactc ggcctgttct tttaaagtgc tccaaccgtt cacgacgcgc tccccgtcgt
    13081 ttttcgatta acaaacgccg taattcctca acagacatgt cttcaggggc tttacgtctg
    13141 ggcatggcga ggggattata ccgcatggca taatgcctca ttgggttgca aattttatga
    13201 cccctcgtta taatacaaat ccttcgggcg ggtagctcag ttggttagag cactgctttg
    13261 acatagcaga ggtcgtaggt tcgagcccta tctcgcccac caaaaaaagt cccctaacgg
    13321 gacttttcgt tttctataac gcgcgccaaa agcgatgctc gatttttgca aatccatgcc
    13381 gttcaaagaa agcatgcgcc ggggtattaa aatcccacga acttagcagt atctttttcg
    13441 ccccaagctt cccggccagc ttcaccgctc gcctcatgag cgccatacca acgccatttc
    13501 gtcgtgcctc cggacgaaca gagatgtgct caagttgcag gaagcgattg gcatgcgtaa
    13561 atggaccttc tggtcgttcg aacaacttgc agaagatata accaattgcg cgctgttttt
    13621 cttcagcgat gaaaatgtga gtcacagcgt ctgtcaaaat ttcctggaag aaggaaacag
    13681 caaagtcgtc atcatgtggc attttgaaaa tttcgggatg atgttcagca tgcaaacgtt
    13741 gtacatccac acataaagag gaaagaagca ggctctccct ggcggtcgct ttgcgagttt
    13801 tcataagctc tcctctgctt gacaggtgtt tttttcggca tataatatca cttatataaa
    13861 cgcagtgacc gagacgagta cgtggtcaaa accctaacag ggagaagaga tcaaagattg
    13921 agagtctctt ccagggaaat cacggaaaac cactcgcgag cgtaaccccg aaatcgaatg
    13981 aaagtatggg aaacggatgg ctccgttaga ggcctaagag atactccaaa ggtggtctcg
    14041 atacaagcga aacaagcact cgctctactc gaccaccgaa atgagtaaat cgggtggaac
    14101 cgcgtgaatc aaattctcgc cccgtggttg ggtgagaatt tttttgttta atttaccccc
    14161 tccgccgcta atgcggcatc tccccaaatc ctttggattt ggggaggaag ggtgggcagg
    14221 aggtcgctat gactaacgat gacactagta tcactcatca gcggctcgga gttatttgct
    14281 gatactcgaa agagtacatg ctgccgcttc tccgagttcg cgcagacaca tggaagcggt
    14341 agcagcgaaa aatcaagaaa ggaagtagca atgtcgcaac aagttcaaga aaaatacgaa
    14401 gagtcgcagc tttataaaat caggcactcg gccgcgcacg tgatggcgca ggccgtgatg
    14461 gagatgttcc cagacgggaa gatcacgatc ggtccgcctg tggagaatgg attctattac
    14521 gacttcgatc tgccgcgcaa cctcacgcct gaggatctgg cagccatcga aaagcgcatg
    14581 cgacaggtcg tccagggcaa acacgagttt atgaagaaag tcgtctctgc cgaggaggcg
    14641 cgcgagatct tcaaagatca gccctataag atcgagttga tcgatggtct cgaaaaaggc
    14701 ggcctggacg aatatgggaa tccgctcaaa gagaaacctg aaatctcaat ttatcagcac
    14761 gatacatttg tagatctatg tcgtggccca catgtggcaa atacaaaaga gattaagcag
    14821 gatgccttca agttaatgtc cattgcaggt gcgtattggc gcggcgatga aaagaacaag
    14881 cagttgcaac gcgtctatgg cacggcctgg gagaacaaaa aacagcttga agaatacctg
    14941 cacaatctag aagaggcaaa gaagcgcgat catcgcaaat tgggcaggga actcgaaatc
    15001 tatatgtttg atgatgaggt cggtcccgga ctgcctctct tcctgccaaa cggcggcatc
    15061 atgatcgagg aattagagaa acttgccaaa gagatggaag acaaggccgg ttatgtgcgc
    15121 gtccgcacgc cgaatctgac gaaggaggat ctgttcattc gttcgggtca cttgccctat
    15181 tatgcagaaa gcatgtaccc gccgatggag ctggaaggcg tgaagtacta cgtcaaaccg
    15241 atgaattgtc cgatgcatca caagattttc gcatcgaagg gacgttccta tcgtgacctg
    15301 ccgattcgcc tcgcggaata tggaacggtg tatcgctatg aaaagtcagg cgagttgttt
    15361 ggcctgatgc gcgtccgctc gttgcagatg aatgacgcac acatgtacgt cacggaggat
    15421 cagtttgagc aggaattcct cggcgtcgtt gacctgtatc tcaaatactt cgaattgttc
    15481 ggcatcgaga aatatgtgat gcgcctgtcc ttgcacagca aagctgggct tggtaagaag
    15541 tatgtggaca acgagcgc
//
LOCUS       DASTJP010000570         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_720127, whole genome shotgun sequence.
ACCESSION   DASTJP010000570 DASTJP010000000
VERSION     DASTJP010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720127"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFQ23_16705"
     CDS             <1..329
                     /locus_tag="VFQ23_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908290.1"
                     /translation="YKSADRPEVGTIVPFDKIAEVDTTISFVTQARHALYTGRLQDSH
                     ALIAQLKEYEDTSREGYLLEGELAIVDGDHDTARQFLQGLAEGGTTTTPEWIRLFAIE
                     LLKRIP"
     gene            332..1099
                     /gene="tpiA"
                     /locus_tag="VFQ23_16710"
     CDS             332..1099
                     /gene="tpiA"
                     /locus_tag="VFQ23_16710"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET9908291.1"
                     /translation="MNRISVVAGNWKMNKTVAEARDLVTTIRGKLLEISNVEKVLCPP
                     FMAIPALYEMLQSSEIGLGAQNLYWEEKGAFTGEVSPGMVKEFCTYVIIGHSERRTYF
                     GETDETVSRKVSAALKIGLTPIVCVGETLEQYESGLTSEVVRRQIKVGLAAIDPVDPA
                     GARRIIVAYEPVWAIGTGKASSGENANQVHQHVIRPALSDLFGEETAAAIRILYGGSV
                     TAANASEFFAFPDIDGALVGGASLKPDEIVAITKAAA"
     gene            1207..1965
                     /locus_tag="VFQ23_16715"
     CDS             1207..1965
                     /locus_tag="VFQ23_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908292.1"
                     /translation="MLPQLSGLIWFVLMLVPLILLQRLLHREIQAVFLILTRDARLTM
                     GIFSMLFLPGVFLHELSHFVMAKILSVRTGRFSIFPQSLPDGRLQLGYVETAKSDVVR
                     DSLIGAAPLIIGTLFVAYVAIYRLEMRVLWDVLRNGQTDLFWMGIRALPQVGDFYLWF
                     YLVFAVSSTMMPSQSDRHAWQALVISVGVLFAVALLVGAGPWVLSNIAPRVSDFLSSV
                     AVIFGLSTFVHVLLVLPTALVHKILARLTGVDVA"
     gene            complement(1981..2604)
                     /locus_tag="VFQ23_16720"
     CDS             complement(1981..2604)
                     /locus_tag="VFQ23_16720"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9908293.1"
                     /translation="MKRKHRYRSVSRRVWFYLSTPPKGIAFPERRNFESAILSKNHIG
                     LKKRILDELKYKKIFGMKPFHKSLKEPGFLDSERNNVMTRIITSEDCGNSPKNIFVQD
                     LTIAFAKGDSIFLLSKVTDDIRWNKIGDTLIKGKEDFALALDTLKKEEAVQLTVHHVA
                     THGKTGAVNGTIKLKNGKTYAFCDVYEFSNAKAVAAKEITSYVIETK"
     gene            2698..>2991
                     /locus_tag="VFQ23_16725"
     CDS             2698..>2991
                     /locus_tag="VFQ23_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908294.1"
                     /translation="MPTSTHLPLSTATSESPLEESRIQKRISLPVGAGPVAFGGQTIW
                     VSMPSEKQVIGIDIGTGQANGSPVNLNFEPRQIAYGEEAVWVLSTDYSVLARID"
BASE COUNT          662 a          701 c          766 g          862 t
ORIGIN      
        1 tgtataaatc cgccgataga cctgaggtgg gtacgattgt tcccttcgat aagatcgctg
       61 aggtggatac gacgatttct tttgtcaccc aggcacgcca tgcgttgtac accgggcgtc
      121 ttcaggattc acatgctctt attgctcagt tgaaagagta tgaggataca tcacgcgaag
      181 gctacttact tgaaggtgaa ttagcaatcg tcgatggtga tcatgatact gcccgccaat
      241 ttttacaagg tcttgcagaa ggtggaacca ccactacacc tgagtggatc cgcctctttg
      301 ccattgaact cttaaaaagg ataccctgac tatgaatcgc atctctgttg tcgctggaaa
      361 ttggaagatg aacaaaacgg tcgcagaagc tcgtgacctg gtaactacaa ttcgtgggaa
      421 gctccttgag atctccaatg ttgaaaaggt gctgtgtccg cctttcatgg ctatccctgc
      481 actctatgaa atgctacaga gttcagagat tggtctcggc gcgcagaact tgtattggga
      541 ggaaaaaggc gcgttcacag gtgaagtttc accaggcatg gtgaaggaat tttgcacgta
      601 tgtcattatc ggtcattctg aacggcggac ttattttggc gaaacggatg aaacggtgag
      661 tcggaaagtt tctgccgcgc tcaagatcgg attaacgccc atcgtctgcg tgggtgagac
      721 actggaacaa tacgaatcag gtctgacgtc cgaagttgtt cgccgccaga taaaagtagg
      781 tttggccgca attgatcccg ttgaccctgc aggcgcgcgg cggatcatcg ttgcttatga
      841 accggtctgg gcgatcggaa cgggaaaagc ctccagtggc gaaaatgcca atcaggtgca
      901 ccagcatgtg attcgcccgg ccttgagcga tttgtttggc gaggaaactg ctgccgcgat
      961 tcgaatcctg tatggtggtt cggtgactgc ggcgaacgcc tccgaatttt tcgcctttcc
     1021 tgatattgat ggagcactcg tcggtggtgc aagcctgaag cctgatgaga tcgtggcgat
     1081 taccaaggct gcagcataat gaatgtcatt gcgaggagcg tttattgtga cgaagcaatc
     1141 tcctcaaaaa tatggagatt gcttcgggct tgtgccctcg caatgacata ataatcgggg
     1201 agattcatgc ttcctcaact ttcgggtctc atctggttcg tcctcatgct cgtgccgctg
     1261 atattacttc agcggctttt gcatcgcgaa atccaggccg tgtttcttat tcttacccgc
     1321 gatgcgcggc tcaccatggg gattttctcc atgttattct tgccgggggt cttcctgcat
     1381 gagctgagtc actttgtgat ggcaaagatt ttaagcgtgc gaacgggaag attttcgatc
     1441 tttcctcaat cgctgccgga tggacggttg caattgggtt atgtcgaaac tgccaaatca
     1501 gacgtggtgc gcgattcgct cattggcgcg gcgccgctga ttatcggaac attatttgtc
     1561 gcttatgtag ccatctatcg cctcgaaatg cgggtgctgt gggatgtgct tcgtaatggt
     1621 caaacggatt tgttctggat ggggattcgc gcgctgccac aggtcggaga cttttatctg
     1681 tggttttatc tggtgtttgc agtgagcagt accatgatgc cctcgcaatc ggaccgtcat
     1741 gcctggcagg cgttggtgat atcggtgggg gtcctgttcg cagttgcctt gctggttggt
     1801 gctggtccct gggtcttaag caatattgcc ccacgagtga gcgattttct cagttccgtc
     1861 gcagtgatct ttggcttgag cacctttgtg cacgtccttc tcgtactgcc cactgcgctg
     1921 gtgcacaaaa tcctggcacg cctgacgggt gtagatgtag cgtaaaccgt caagttgaac
     1981 ttattttgtt tcaatcacgt aagatgtaat ttcttttgcc gcaaccgctt tggcattact
     2041 aaattcatac acgtcacaaa aagcatacgt cttcccgttt tttaatttta ttgttccgtt
     2101 caccgcccct gtcttgccgt gtgtggcgac atgatgtacc gttagctgaa ccgcctcttc
     2161 cttttttaac gtatccaggg ctaaagcaaa atcctctttt cctttgatga gtgtatcgcc
     2221 gattttgttc cagcggatat catctgtcac tttgctgagt agaaatatag aatcgccctt
     2281 tgcaaacgcg atggtaaggt cttgcacaaa aatgtttttt ggggagttgc cacaatcttc
     2341 gcttgtaatg attctggtca taacgttatt cctttcgcta tcgagaaatc ctggttcttt
     2401 cagggactta tggaatggct tcatgccaaa tatttttttg tatttcagct catctaagat
     2461 tcttttttta agcccgatgt ggtttttgga gagtatagca gattcaaaat tacgcctttc
     2521 agggaacgca atccctttag gcggtgtaga aaggtaaaac caaacccgtc tactcacgct
     2581 acgatatcta tgttttcttt tcactattcc aagcgactaa cctggctttt tgtaatcttc
     2641 ctcatccctt gtctcttgtc ctgtgacgcc aaccccttgc cggagtcgga agggcaagtg
     2701 ccgacttcga ctcatttgcc attgtcaacg gcaacctcag aatctccact agaggaaagc
     2761 agaatccaga aacggatttc tttacctgtg ggggcaggcc ccgtcgcgtt tggcgggcaa
     2821 accatctggg tttcgatgcc ttccgagaag caagtcattg gaattgatat aggaacaggc
     2881 caagctaatg gttcccctgt caacctgaac tttgagccgc gccaaatcgc ttatggggag
     2941 gaagcggttt gggtgctaag cacggactat tctgtcctgg ccaggattga t
//
LOCUS       DASTJP010000571        14073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_885013, whole genome shotgun sequence.
ACCESSION   DASTJP010000571 DASTJP010000000
VERSION     DASTJP010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14073
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885013"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..829
                     /locus_tag="VFQ23_16730"
     CDS             122..829
                     /locus_tag="VFQ23_16730"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908295.1"
                     /translation="MSNQQINEAFEGLSTPLIADACLRLEIPLRLAPTGIRPISIRHH
                     IAGRVLPVRHYGSVDIFLEVMGHSQPGDILVIDNNGRGDEGCIGDLTALEAQATGLAG
                     IIVWGVHRDTSELIEIDFPIFSYGTCPAGPQRLDPREAEALTAAHFGELELTNEDVAF
                     ADGDGVLFLRSEHAEQVLSMSREIWEKERRQAQKIKSGQRLREQLLFDDFLAKRSVDA
                     TYTFRQHLRSVGGAIEE"
     gene            829..1272
                     /locus_tag="VFQ23_16735"
     CDS             829..1272
                     /locus_tag="VFQ23_16735"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908296.1"
                     /translation="MEIIRAQARDADVIAPLFDAYRQFYKAPSDLNASRQFIFDRLAK
                     DESVIFLAMEGNHALGFTQLYPLFASVALQSLWLLNDLYVAPEGRKQGVGEALIKHAE
                     QFAHETGSRGLFLRTATDNFPAQQLYEKCGWVRDEQFYRYDKILK"
     gene            1291..1686
                     /locus_tag="VFQ23_16740"
     CDS             1291..1686
                     /locus_tag="VFQ23_16740"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9908297.1"
                     /translation="MKNITREEFVELLQRLADGWNQGNAQQAAECFTQDAIYTEPPDK
                     QYYNGREALYQFFGGPNGRAGEMNMAWHHIIFDETDQLGSGEFTFTYGDTVHGVTMVK
                     VRNGLISNWREYWYTSDLLWEEFTQLNSF"
     gene            1888..2790
                     /locus_tag="VFQ23_16745"
     CDS             1888..2790
                     /locus_tag="VFQ23_16745"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HET9908298.1"
                     /translation="MLSHKIIDGKKQAQIVRDEVRVRVGALKDMGWAPRLISIDIGEI
                     GAVRLFIKNQQKACADVGIEYENRHYPADVTQREVLAAIYALNADPRVTGIILQRPVP
                     RTLDLEELENAMHPSKDVEGMSSANIGNIVYGDSSLGPCTSLASVELLRSTGLNLQGL
                     EVVVVGHSEIVGKPVALLLVEELATVTICHHGTRNLSYHTRQADALIVAVGKPSLITA
                     DMVKPGAAVIDIGINQVDALLPDGTMGKKTVGDVDFEGVHQVAGWITPVPGGVGPMTV
                     AMLLRNTAVATERQKKRYEEVMRV"
     gene            2787..3572
                     /locus_tag="VFQ23_16750"
     CDS             2787..3572
                     /locus_tag="VFQ23_16750"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9908299.1"
                     /translation="MNCRITHISTATVLLEIGSVRILTDPVFDPAGGNYQFGYGTGAR
                     KLTAPSIKPASLGKIDAVLLSHDHHEDNLDQAGRAFLPNAEKVITTVAGAKRLGNNAV
                     GLKPWQTITISSEDDDSEIKITAVPARHGSLWSHLIVGETCGFVLEWEGQKHGALYIS
                     GDTVWFNGIHEIAHRFNIGTILLHIGKASFPITGPIRFTLTAGEAVRIIRVLDPHMAI
                     PVHYEGWNHFREPREKAEAHFIEADLEETVQWLPLGIPVNIEV"
     gene            3623..3970
                     /locus_tag="VFQ23_16755"
     CDS             3623..3970
                     /locus_tag="VFQ23_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9908300.1"
                     /translation="MDLVKITNVEGRIPVTVFQLQDRVNLGNFAELEQTAKDAYEAGM
                     RDLVIDLSQTPSLTSIGIRAIVIIHRLLSEDRGKHLKLAGALVPIREMLGIAGITQFI
                     EMYETVDEAVASF"
     gene            4278..6152
                     /locus_tag="VFQ23_16760"
     CDS             4278..6152
                     /locus_tag="VFQ23_16760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908301.1"
                     /translation="MEPEAKFPTFTGNQSLRQSALTESIHTTPNTSVPAISKKWGQGK
                     YSPGYNLLVTVGGIAIAEAIAMVVVYFYRFLPYYQQVLLDTALMTAIISPLLYVLSTL
                     PLLRHIRQRTQTESILESRLRLIQTSNTQTLDQLLQATLDEIEALTGSTVSYFHFLKS
                     DQKTLWLRAWSTKTLQSMHILEGDDPCHELEQADIWSDCVREQRPILYNDRSLLPHYK
                     SGFDTYAQITREMAVPIMRDGKIVAILGLGNKPQNYSEADVELVSTLADFVWDIIKHR
                     EAGDALRESEEKFRTLVDWTYDWEIWLDPQGNIIYNSPSCERITGYKPEEFIAASDLI
                     ISIINPDDRLLYQNHQKVSHDKSAGLEEVEFRVIARDGQQHWIEHICRPLFGEENHYL
                     GRRVSNRDITERKQAERDIQERNQKEKILTQTIHTMQLDIARDLHDTVGQNIGFLRMK
                     LDHMSEMGLQSQTDIQEEIRILSEVANESYDLIRGTLAILQSESSADLFRLFIRYAEQ
                     IQERASFKVDFSSLGNPRLLSARRMRQLFYIFREALNNIEKHASASHVSVDMMWEHDH
                     LAFVIFDNGKGFDINRAQYGTHYGLKFMKERAQILSGTLVIHSTVNSGTKIEVRVPYE
                     "
     gene            6179..6814
                     /locus_tag="VFQ23_16765"
     CDS             6179..6814
                     /locus_tag="VFQ23_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908302.1"
                     /translation="MKIVIVDDHALFREGLASILRSEPDIEIVGMAGTVKEAIEISCV
                     AHPEIILMDFTLPDGTGAEATRLILDNQPQSKIVFLTMSEEDEDLFTAVRSGAKGYLL
                     KNMQPKKLITALRSVQKGESALSRSMTLRLMEELSRTKRPESSPDVTLTHREMEIFRA
                     LASGLSNQEIAKQYYISENTVKYHVHSILGKLGLGNRRELTEYAHEHRLID"
     gene            6969..7412
                     /locus_tag="VFQ23_16770"
     CDS             6969..7412
                     /locus_tag="VFQ23_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908303.1"
                     /translation="MKLTELFAELKSQLDSDPSTLRKAMIWGREDLLGGAIESLLRST
                     SHWQVIRIPGNPDTEWLRQEVEKALPSVLVIIQGEYDEEFPLPLQLIQTFSELKIITV
                     NPLNNRIEVYNRQKIWIKEASDLLSIIEEDADSDRRGVKSTTKKN"
     gene            7486..12381
                     /locus_tag="VFQ23_16775"
     CDS             7486..12381
                     /locus_tag="VFQ23_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET9908304.1"
                     /translation="MNTRKFLNILVSFMVITTLFAGNSSNATAMPMNPTDETKVPHYF
                     GPYPNWANSPFTLPDVQVVITGDGTGATAEATVGANGAITGLTITNPGHNYNNAKVDI
                     FGSGTGATADVTIVRRGAVVAVTVDAAGSGYTAPTVAFSGNGGAAATAYGGVDQVTVT
                     SGGSGYTFPTVEFDLPDGPDGVQAKGHAEMNANGTITAVIVDSPGSGYSFAPGVAIHN
                     GTLYDPIAGATVATATTTLAISSIVMDAFGADYNNPPTVDIIDPTGTGALATAVLDNG
                     IISGIFVKRAGSGYLIPGMRKFVDTLPGLGAGAANNLGQYIPVAVPDTTSYPGSDYYE
                     IELGQYTEQMHSDLPPTLLRGYRQTNMGGTAFHFLGPLIIAQKDRPVRIKFTNKLPTG
                     TGGDLFLPVDSTLMGSGEGNMSATYDPVPAMGGDVMDTSRNPMCSDYPKMADCFKDNR
                     ATLHLHGGNTPWISDGTPHQWITPAGENTPWPQGVSVANVPDMNMCSATDDGCQTFYY
                     SNQQSARLMFYHDHAWGITRLNVYAGEAAGYIITDPTEQKLFGAGGPYAGMGEGIPLI
                     IQDRTFVPKAEQLAWQDPTWDSSRWGGFGNFWYHHVYMPAQNPGDPSGMSAFGRWMYG
                     PWFWPPAGDVKHGPIANPYYGKDPEGPDGVRGTADDWTTLLATPCTLDDSTTWQYQEE
                     PFCEPEQIPGTPNISVGMEQFNDTPMVNGTAYPTVTLEPKSYRFRVLNAANDRFFNFQ
                     WYVADATTASTGLNALGQVIGGTEVAFNADQLAAAQLDPVVFPTPDLTLSPAGPDWIH
                     FGTESGFLPKPVLVDGQQVTTWITDPTRFDVGNVDQHSLLLAPAERADVIVDFSRFAG
                     KTLILYNDAPAAFPARIPSYDYYTGAPDLSPVGAPTILPGYGPNTRTIMQVKIAGTTA
                     PAFNVSQFQTRFSHKADGSGVFEEGQHPIIVGQAAYNSAYGTSFASGGYCSSPNSTNK
                     CDGFARINQQGGDPFTFDTLKGATTGTWSKLSIPLEPKAIHDEMNSTNFDEFGRMQAT
                     LGVEVVPATPGLQNVVMSPFVLPTTELIDTTNLPKADVKVTPIATADDGTQIWKITHN
                     GVDTHPIHFHATDVQVLNRVTWDNIIIPPDSTELGWKETVRVAPLEDTIVAMRPIIPT
                     LPFDFPNSIRLLSPMMPDGAAIDLSSVKDLLGLGMSAFAPNGEPIDVYNHYVNFGAEY
                     VYHCHILSHEEMDMMHSVLYAFPPVAPGGLTFNTGTVSWMDNSRSETAFIVEKSQDGG
                     ISWTEIGRVDRLLAPDAGSSTGGTESFTDTAWVAGDQYRVVAQNTVGDTHDYSNPNFN
                     ELVPGTFAFPVVTTTAISEVLDSTTPPAPPAAPSGLTAIAETGPQVSLAWTDNATDET
                     GLLVQRSTDGVNFTDLTTLPANSIGYTDTAVIAGTTYTYQVRAFNTGGPSAFSNIASA
                     TIPGLQGPTAPSELVANLQDTPLIDLAWVDNSTDETSFVIERSTDGVTFSVVANVGAN
                     VTAYNDLAVFGGYTYTYRIAAVNANGASAYAISNPVTVPPDATAPAAPSEVTATNLTQ
                     TTLTLSWLDNSNNETGFTIQRATNSSFTRNLVTFTVGADVTSFNDNGLRRNTRYFYRL
                     VAFNVNFTSPWSPTLNVTTPR"
     gene            complement(12468..13505)
                     /locus_tag="VFQ23_16780"
     CDS             complement(12468..13505)
                     /locus_tag="VFQ23_16780"
                     /inference="COORDINATES: protein motif:HMM:NF027787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HET9908305.1"
                     /translation="MESPKKILPIFDALLFGGLGCGVLGLCILAGAFVYIWQNPLVSR
                     VEPTQIDFSASPVVSTPFLDLSTPTLSPTIVPSLIPSPVPGTSLGGKIVFTCFIEQID
                     QICIVNADGTGRRQLTNLSATAFYASLSPDGQTIYFSSRHSGSYEIYSIDVNGNNLQR
                     LTQNIGTLYAPELSPDGSKIIFTSNGNGVWVMNADGSDPHAITFRDDIDPTWSPDGSM
                     IAFASNRSGTRQLYTAKASGKQVNQVTDLTNMGGRSTWSPNGKWLAFYRGPEGDRDIY
                     VIKIDGSGLERLTNGGDNLGPSWSPDGEWIAFTSFRDGNNEIYKIRPNGTGLTRITNS
                     LTSDWQPRWGR"
     gene            complement(13586..>14073)
                     /locus_tag="VFQ23_16785"
     CDS             complement(13586..>14073)
                     /locus_tag="VFQ23_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908306.1"
                     /translation="QKIWTMRADGNNPHPITNGPYDDIDPTWSPDGAYIAFASSRTGQ
                     RQLFVMERDGTEIRQLTNLPDMGGRSTWSPNGRQLAFYAGPAGNHNIYVINADGTGLV
                     QLTNGGDNLGPSWSPDGNWITFTSFRDGNNEIYIIRPDGTGATRVTNSAISDWQPRWG
                     R"
BASE COUNT         3624 a         3627 c         3574 g         3248 t
ORIGIN      
        1 tggcagctgc gctggggctg gtctcggcgt tcttcacgcc gcgcaaagaa ctcacggata
       61 caagatcctc aacaagagag agcactcccg cacctgttag cgcggactga tcattgaccg
      121 tatgagcaat caacaaatca atgaagcgtt tgaaggactt tcaaccccat tgattgcgga
      181 tgcctgtttg cgcctggaga ttcccctccg actggcacca acaggcattc gtcctatatc
      241 catcaggcat cacatcgccg gtcgagtctt gcctgtgaga cactatggca gtgtggatat
      301 ctttctcgaa gtcatggggc attcccagcc tggcgatatt cttgtcattg ataacaatgg
      361 aagaggcgat gaaggctgca tcggcgattt aaccgcgctg gaagctcagg ccacagggct
      421 ggctggcatc atcgtttggg gtgtgcaccg cgatacgagc gaactcatcg aaattgactt
      481 tccaattttc agctatggaa cctgcccggc cggtccgcag cgactcgatc cgcgggaggc
      541 agaagcattg accgctgccc attttggtga gcttgaattg actaatgagg atgtggcttt
      601 tgctgatgga gatggcgttc tattcttgcg aagtgaacat gctgagcagg tgctttccat
      661 gtctcgcgag atttgggaga aagaacgtcg tcaggcacaa aagataaaat ccgggcagcg
      721 actccgcgag cagcttttat ttgatgactt cctggcaaag cgatccgtgg acgccaccta
      781 tacattccgg caacacctgc gttctgtggg cggagcgatt gaagaataat ggaaatcatt
      841 cgcgcacaag cacgggacgc agacgtgatc gcgccgttat tcgatgcgta caggcaattc
      901 tacaaagcgc catctgatct caacgcatcg cgtcagttta tctttgaccg actcgcaaag
      961 gatgagtcgg tcatctttct ggcaatggaa ggtaaccacg cacttggttt tacgcagctg
     1021 tatcccttgt ttgcttcagt cgcattgcaa tcgctatggc ttttgaatga tctctatgta
     1081 gctccagaag gacgaaagca gggtgtaggg gaagcattga taaaacacgc cgaacaattt
     1141 gcacacgaga ccggcagtcg cggtttattt ttgcgcaccg ccaccgataa tttcccggca
     1201 caacagcttt atgaaaagtg cggctgggta cgagacgaac agttttaccg ttatgacaaa
     1261 atcctgaaat agccaatcag cggaggtggc atgaagaata tcacccggga ggaatttgta
     1321 gaactgctgc aacgtttagc tgatggctgg aatcagggaa atgctcaaca agcggcagag
     1381 tgctttacac aggatgcaat ctataccgaa ccgcctgaca aacaatatta caacggtcgc
     1441 gaggcgttat atcagttctt tggcggacca aacggcaggg ccggggaaat gaacatggcc
     1501 tggcatcaca ttattttcga tgaaacagac caacttggct caggcgagtt tacctttacc
     1561 tatggtgata ctgtccatgg cgtgacgatg gtaaaagtgc ggaatggctt gatcagcaac
     1621 tggcgggaat actggtatac ttccgacctt ctctgggaag aatttacaca attgaacagc
     1681 ttttgaaccg gttgatactt tcaaatcaaa tggtggttga gtagcccgaa gtgatagcga
     1741 agggcgtatc gaagccacaa aacctagcta ttgatatttc ctgttggttt cgatacggcg
     1801 gtttgctctg cgcaccgcct actcaaccaa cgaaactatt ttggtgtatg attgcgaaga
     1861 tttccacaca acagccaaat ggtagccatg ctctcacaca aaatcatcga cggaaaaaaa
     1921 caggctcaga ttgtcagaga cgaagttcgc gtacgagtgg gggcactcaa agacatgggc
     1981 tgggcaccac gtctcatttc gatagatatc ggtgagattg gtgcggtacg acttttcatt
     2041 aaaaatcagc agaaggcctg cgcggacgta ggcattgaat atgagaatcg ccactatcca
     2101 gccgatgtca ctcagcgcga ggtgctggcg gcgatttacg ccctaaatgc cgacccgcgc
     2161 gtgacaggca tcattttgca gcgccctgtc ccacgcactc tggacctgga ggaattagag
     2221 aacgccatgc atcccagcaa agatgtggaa gggatgagct ctgccaatat cggcaacatc
     2281 gtctatggcg attcgtcgct gggaccttgc acctcgctgg cgtcggtaga attactgcgc
     2341 tctacgggtc tcaacttgca aggactcgaa gttgtagtag tgggtcactc cgaaattgtg
     2401 gggaaaccgg tcgcattact gctcgtggaa gaattagcaa ctgtcacaat ttgtcatcat
     2461 gggacgcgca atctctcgta tcatactcgt caggccgatg cgctcatcgt cgcagtgggc
     2521 aagcccagtt tgatcacagc cgatatggtc aaacccggcg cggcagtgat cgatatcggc
     2581 atcaatcaag tagatgccct tttaccagac ggaacgatgg gcaagaaaac cgtcggcgac
     2641 gtagattttg aaggtgtcca tcaggtggca ggctggatca cccccgtacc gggcggtgtg
     2701 ggcccgatga cggtggcgat gctcttacga aacacggccg tggcaacaga acgtcagaaa
     2761 aaacgttatg aagaggtgat gagagtatga actgtcggat tacgcacatt agcacggcga
     2821 ctgttctttt ggaaatcggt tcagttcgaa ttctgactga ccctgtattt gatcccgcag
     2881 gtgggaatta tcaattcggc tatggcacgg gtgccaggaa gttaactgct ccatcgatca
     2941 agcccgcatc cctcgggaaa atcgatgccg tacttttgag tcatgatcat cacgaagata
     3001 atctggatca ggccggacgt gcgtttttgc cgaatgccga gaaggtcatt acaaccgttg
     3061 ccggtgcaaa gagattggga aataacgccg ttggtttgaa accctggcaa actatcacga
     3121 tttcatcgga ggatgatgac tctgaaataa aaatcacagc ggtgcccgca cggcatggct
     3181 cactctggag tcatctgatt gtcggggaga cctgtggctt tgttctggaa tgggaaggtc
     3241 aaaagcacgg agcgctttat atttcagggg atactgtctg gttcaatggg atccatgaaa
     3301 tagcccaccg attcaatatt ggaacgattc ttcttcatat tggtaaagca agttttccaa
     3361 ttacgggtcc gattcgattt accctgactg ccggagaggc ggtccgcatc atacgcgtgc
     3421 tcgatcctca tatggcaatc cctgttcatt atgagggttg gaatcacttt cgagagccac
     3481 gcgagaaagc cgaagcacac tttatcgagg ctgatcttga ggaaacagta caatggctcc
     3541 cactgggtat tccagttaat atcgaagtct agtttaagta gaattctttt gaagtgattt
     3601 actttcagga aggatgatga acatggattt ggtgaaaatc acaaacgttg aaggacgcat
     3661 tcccgtaacg gtatttcaac tgcaggaccg ggtcaattta gggaattttg ctgagctgga
     3721 gcaaactgca aaagatgcct acgaggctgg aatgcgcgac ctggtgattg acctcagcca
     3781 aactccgtca ctgaccagta tcggcattcg cgccatcgtc attattcacc gtctcctttc
     3841 agaagacaga gggaagcatc tcaagctggc cggtgccctt gtccccatcc gtgagatgct
     3901 ggggattgct ggcattactc agttcatcga aatgtatgag acggtggatg aagcggtagc
     3961 gtctttttaa gaaaataact ccttttcttc cacgttgtga tgatagccgc aaaggctggc
     4021 gctcgcctgt ctctgtcttc tttctcttaa tgcgatttgt tgaaaacggg acgatcggtc
     4081 tcctcagcca tgtcggggat agctactggc tatatttaca gcaaatgggt catgttaaac
     4141 cctaaaactg tgacatctgt cactagcttc gcatccccac ccatactaag ataagtgtca
     4201 atcactaaac gaacgcttac caaagcgacg ttacatgctt gcaagggtat tggcttttga
     4261 ggaggctatg atcaaccatg gagccggagg ctaaatttcc caccttcacg ggcaatcaat
     4321 cattgcgaca gtctgccttg acggagtcaa ttcacaccac acccaataca tccgttccag
     4381 cgataagcaa gaaatggggg caaggaaaat attcgccagg ctataacctg ctcgttacag
     4441 tcggagggat agcgattgca gaggcaattg ccatggtcgt tgtgtacttt tacagattct
     4501 taccctacta tcagcaggta ttactagata ctgcgctgat gacggccatc atctccccgt
     4561 tgctttatgt cttgtccacc ttgcccttac tccggcatat tcgacagaga actcagactg
     4621 aaagtattct cgagtcgaga ttgagattaa tccaaacttc caatacacag actcttgatc
     4681 agttgttgca ggcaactctg gatgaaatcg aagcccttac cggaagtacg gtcagttact
     4741 tccattttct gaaatctgac cagaagacgt tatggctgcg ggcatggtca acgaaaacac
     4801 ttcaaagtat gcacatcctg gagggcgatg atccttgtca tgagttggaa caggctgata
     4861 tctggtcaga ttgtgtccgc gagcaaagac ccatcctgta caacgatcgt tccctgctgc
     4921 cacactacaa atcagggttt gatacctatg ctcaaattac tcgtgaaatg gctgtcccaa
     4981 tcatgcgaga tggaaagatt gtggcaatcc tgggcttagg gaataaaccg caaaattata
     5041 gcgaagcaga tgtcgaactg gtttccaccc tggcagactt tgtgtgggat attatcaaac
     5101 atcgagaggc gggagatgca ctgcgcgaaa gcgaggaaaa attccgcaca cttgtcgatt
     5161 ggacttatga ttgggaaatc tggctcgacc cacaaggcaa tattatctac aattcacctt
     5221 cctgtgaacg tattacgggc tacaaacctg aggagtttat tgctgcttca gatctgatca
     5281 taagtatcat taatccagat gacaggctgc tctatcaaaa ccatcagaaa gtcagccatg
     5341 ataagtctgc cggcttggag gaagtggaat tccgtgttat cgctcgcgat ggacaacagc
     5401 actggatcga gcatatttgc cgccctcttt ttggcgaaga aaaccattac ctggggcgcc
     5461 gcgtcagcaa tcgagatatc actgagagaa agcaagccga gagggatatt caggaacgca
     5521 atcagaagga aaaaatactt actcaaacga tccataccat gcagttggat attgcgcggg
     5581 atttgcatga taccgtcgga caaaatatag gtttcctgag aatgaagctc gaccatatgt
     5641 ctgagatggg cctgcaaagc cagaccgata tccaggaaga aatccgcatc ctgtcggagg
     5701 tggcgaatga gtcatacgac ctgattcgag ggacgctggc catacttcaa tctgagagtt
     5761 cagcggattt gttccgttta ttcatccgct atgcggagca gattcaggag agagcatcct
     5821 tcaaggtcga cttttcgagc ctggggaacc caaggttgct gtctgccaga cgcatgcgac
     5881 agctattcta tatcttccgc gaagctctaa ataatatcga gaagcatgca agtgccagcc
     5941 atgtgtctgt cgatatgatg tgggagcatg accaccttgc atttgtcatt ttcgataatg
     6001 gcaaggggtt tgatataaac cgggcccaat atggcactca ctacggctta aaattcatga
     6061 aagagcgggc acagattcta agtgggacac ttgtcattca ttcgacagtc aactcaggta
     6121 cgaaaataga ggttcgcgtc ccatatgaat aattcatcct tcaaaggcga atcacccaat
     6181 gaaaatagtt atcgttgacg atcatgccct ctttcgggaa gggctcgcct ccattttgcg
     6241 gtctgagcca gatattgaga ttgtcggaat ggctgggaca gtaaaagaag ccatcgaaat
     6301 ttcctgcgtc gcccatccgg agattatcct gatggacttt acattgccag atggaacggg
     6361 cgccgaagca acccgcctta tccttgataa tcaacctcaa agtaagatcg tctttttgac
     6421 catgtcggag gaggatgaag acctgttcac cgcagtccgc agtggggcaa aaggatattt
     6481 actcaaaaac atgcaaccga agaaactgat taccgcgttg cggtctgttc aaaaaggtga
     6541 aagcgcactt tcaaggtcga tgaccttgcg tttgatggag gaattgtccc gaacgaaacg
     6601 gcccgaatcc tccccggatg taacccttac tcaccgagaa atggagatat ttcgcgcgct
     6661 ggcttctggc ttatcgaatc aggagatcgc gaagcaatat tatatttccg aaaataccgt
     6721 aaaataccac gtccactcca tacttggaaa gcttggtctc ggaaatcgac gagaattgac
     6781 agagtatgcc cacgagcatc gcttaataga ctaaaagaga gttaaccggg ggattaagaa
     6841 atgacgcaaa aagaccatcc tggtgatggt ctttttttaa tccttacgac agggcaacga
     6901 tggatgcaat agctcctggt tcttcttatg atggattcaa tattttgcat ctcaggacga
     6961 tgcccctcat gaagctcaca gaattatttg ccgaattaaa gagccagctt gatagcgacc
     7021 catcgacatt aagaaaagcg atgatctggg gacgcgaaga tctgctggga ggggcaattg
     7081 aatctctttt acggtccaca agccactggc aggtgatcag aattccgggc aatcctgata
     7141 cggagtggtt gagacaggaa gtggagaagg cgttgccaag cgtgttggtc atcattcaag
     7201 gggaatatga tgaggaattt cctcttccac tgcagctcat acagaccttt tctgaattaa
     7261 agatcattac agtcaacccg ctgaataatc gaatcgaagt ttataacagg caaaagatat
     7321 ggatcaaaga ggcatctgac cttctatcca ttatcgaaga ggatgctgat tcagacagaa
     7381 ggggggtgaa atcaaccact aaaaagaatt gatacaccag atttcccagt aaaaactgcg
     7441 ggaaaacttt gaaaagaata attcgtgaat acataaggag atagagtgaa cactcgtaaa
     7501 tttctaaata tccttgtctc gtttatggta attaccactc tgttcgctgg gaatagttct
     7561 aatgccacag ccatgccgat gaatcccact gacgagacaa aagtccctca ctactttggt
     7621 ccctacccga actgggcaaa cagccccttc acactgcctg atgttcaagt ggtcatcaca
     7681 ggcgatggca ccggagcaac agcagaagcc acggtcggtg caaacggtgc cattacggga
     7741 ctcaccatta ctaacccagg acacaactat aacaacgcga aggttgatat ctttggttcc
     7801 gggacaggcg ccacggccga cgtgaccatt gtcagaagag gggcggtcgt tgcagtcacc
     7861 gttgacgctg ccggttccgg ctacactgcg cccactgttg ccttcagcgg aaacggaggc
     7921 gcagcggcga ctgcctacgg cggcgttgac caggtcaccg ttaccagcgg aggcagtgga
     7981 tatacatttc cgacggttga gtttgattta ccagacgggc cagatggcgt tcaagccaag
     8041 ggccatgccg agatgaatgc aaacggcacc atcacagccg tcattgtaga cagcccgggc
     8101 tccggttact cgttcgcccc gggtgtcgcc attcataatg gaacattgta tgacccgatt
     8161 gccggcgcaa cagtagctac tgcaactaca accctggcga tcagctcgat cgtcatggat
     8221 gctttcggcg ccgactataa caatccgccc acagtggata tcatagatcc cactggaacg
     8281 ggagcgttgg caaccgcggt cctggataat ggcatcattt ctggcatctt tgtgaaaaga
     8341 gctggctcgg gctacctcat tccgggcatg cgcaagttcg ttgatacact gccagggttg
     8401 ggtgctggcg ctgcaaataa ccttgggcag tatatccctg tcgcagtgcc tgacaccacc
     8461 agttacccag gctccgacta ttacgaaatc gagctcggac aatataccga gcaaatgcac
     8521 tccgacctgc cacccaccct gttgcgcggc taccggcaga cgaacatggg tggcactgcg
     8581 ttccattttc ttggaccatt gatcatcgct caaaaggatc ggcccgtccg catcaaattc
     8641 accaacaaac tgcccacagg cactggcggt gatctgttcc tgcccgtcga ctcaactctc
     8701 atgggttcgg gtgaaggcaa catgagcgcg acgtacgatc ctgtaccggc aatgggtggt
     8761 gatgttatgg atacatctcg caaccccatg tgcagtgatt acccgaagat ggctgactgc
     8821 ttcaaagaca accgcgcaac attgcacctg catggaggca atactccgtg gatcagcgat
     8881 gggacacccc accaatggat caccccagcc ggagagaata caccctggcc acaaggtgtc
     8941 agcgttgcaa acgttccaga catgaacatg tgtagtgcaa cagatgatgg ctgccagacg
     9001 ttttattaca gcaaccagca aagcgcgcgt ctcatgttct atcatgacca cgcctggggt
     9061 atcacgcgcc tgaacgtcta tgcgggtgag gctgcgggct acatcatcac tgacccgact
     9121 gagcaaaaat tattcggggc gggaggtcca tacgctggca tgggagaagg aattcctctc
     9181 atcatccagg accggacgtt cgtcccgaaa gccgagcagc ttgcctggca ggacccgacc
     9241 tgggattctt cccgctgggg cgggtttggt aacttctggt atcaccacgt ctatatgcct
     9301 gcccaaaatc ccggtgaccc aagcggcatg agcgcctttg gtcgctggat gtatggtccc
     9361 tggttctggc ctcctgctgg agacgtgaaa catggcccga ttgccaaccc atactatgga
     9421 aaagatccgg agggcccaga cggagtcagg gggacagcgg atgattggac caccttactg
     9481 gcaacgcctt gcactctgga cgactcaacc acgtggcaat accaggaaga gccattttgt
     9541 gaaccggaac aaattcccgg cacgccaaac atctcggtcg gcatggaaca attcaatgac
     9601 acaccgatgg tgaacggtac agcgtacccg actgtcaccc tggagcccaa gtcctaccgc
     9661 ttccgtgtcc tcaacgcagc caacgaccgt ttcttcaact tccaatggta tgtggctgat
     9721 gccaccaccg cgagcacagg gttgaatgca ttgggacaag tcattggcgg aaccgaagtg
     9781 gcattcaacg cggatcagtt ggctgccgcg cagctcgacc cggttgtttt cccaacacca
     9841 gacctgacgc tgagtcccgc gggacctgac tggattcatt tcggcacaga atctggcttc
     9901 ctgcccaaac cagtcctggt ggacggccaa caggtcacta cctggatcac cgatccaacc
     9961 cgcttcgatg ttggcaacgt ggaccagcac tcgctgctgc ttgcacctgc cgagagggct
    10021 gacgtaatcg tcgacttctc gaggttcgcc ggtaagacct tgatccttta caacgacgca
    10081 ccagcagcgt tccctgctcg tatcccaagt tacgattatt acactggcgc cccagactta
    10141 agtccggttg gcgcgccgac catcctgccc ggatacggtc caaacacccg caccatcatg
    10201 caggtgaaga tcgcaggcac tactgctcct gcgttcaacg tctcacaatt ccaaacgagg
    10261 ttcagtcata aggccgacgg ctcgggtgtc tttgaagaag gacaacatcc gatcattgtt
    10321 gggcaagctg cctacaactc tgcctatgga acgagtttcg cttccggcgg ctattgttca
    10381 tcgcccaatt cgaccaacaa atgcgatggg ttcgcgcgta tcaatcagca gggtggcgat
    10441 ccgttcacat ttgacaccct gaaaggagcc accaccggaa cctggtcgaa gctatccatt
    10501 ccgctcgaac cgaaagccat ccacgacgag atgaactcga cgaactttga cgagtttggg
    10561 cgtatgcagg cgaccctggg tgttgaggtc gtcccagcta cgccaggatt acagaacgtt
    10621 gtcatgtctc cctttgtgct cccgacaaca gagctgattg acacgacaaa cctgccaaag
    10681 gcagacgtga aagtgacgcc cattgctaca gcagacgatg gaacacaaat ctggaagatc
    10741 actcataatg gtgtagatac gcaccccatc cacttccacg cgaccgatgt tcaggttcta
    10801 aatagagtca cctgggacaa cattatcatt cctccagact ccacggaact gggttggaag
    10861 gaaacagtgc gggtcgcgcc gctcgaggac acgatcgttg ccatgcgtcc catcatcccg
    10921 acgttgccgt tcgacttccc caacagcatc cgcctgctca gcccaatgat gcctgatggc
    10981 gcggcaattg atctgtcatc ggtaaaggac ctattggggt tgggaatgtc agcgttcgcc
    11041 ccgaacggtg aaccaatcga cgtttacaac cactacgtta acttcggcgc cgaatatgtt
    11101 tatcactgtc acatcctgag ccacgaagaa atggatatga tgcactccgt gctgtatgcc
    11161 ttcccgccag ttgctccagg tggtctgacg ttcaacacag gaacggtgtc ctggatggat
    11221 aactcgcgca gcgaaaccgc cttcattgtt gaaaaatctc aggatggagg tatctcgtgg
    11281 accgagattg ggcgagtgga taggctttta gccccagacg ctgggagcag caccggaggc
    11341 accgaaagct tcacggatac agcatgggtc gcaggcgacc agtatcgcgt cgtcgcgcag
    11401 aatacggttg gcgacacgca tgactactct aacccgaact tcaacgagct agtgcctgga
    11461 acattcgcat tcccggtggt cacaaccacg gccatctcgg aggtgctcga tagtactacg
    11521 cctcctgcac cgccagcagc gccatccggg ctgacagcca tagcagagac gggacctcag
    11581 gtcagccttg cctggacaga taacgccacc gacgagacgg gcctcctcgt ccagcgctct
    11641 accgacggcg tcaactttac tgacctgacc accctacccg ctaacagcat tggctatact
    11701 gacaccgctg tgatcgccgg cacgacctat acgtatcagg taagggcctt caatacaggt
    11761 ggcccatctg ccttctctaa cattgccagc gccaccatcc ctggactcca aggtcccact
    11821 gcaccatctg agctggtagc caatttacaa gacaccccac tgatcgatct ggcctgggtg
    11881 gacaactcca ccgacgagac cagcttcgtg attgaacggt caacggatgg tgtcaccttc
    11941 tctgtagtag cgaatgtagg ggccaatgta acggcgtaca acgacctggc tgtctttggc
    12001 ggctacacct acacgtatcg gattgcggct gtcaatgcaa atggcgcctc agcctatgca
    12061 atctcgaatc cagtgactgt gccacccgat gcaactgcac ccgcagcacc ctcggaggtg
    12121 acagcaacca accttactca aacaacgctg acgcttagct ggctggataa ctcaaacaat
    12181 gagaccggct tcaccatcca gagagcgacc aatagcagct ttaccagaaa ccttgtgacc
    12241 tttaccgtgg gagcagatgt gacatccttt aacgataacg gcttgaggag gaacaccaga
    12301 tatttctacc gcctcgttgc tttcaatgta aatttcacat ccccgtggtc gcctaccttg
    12361 aacgtcacga caccaagata agccagatta attaaccttt tgaggaggga cgcgaatacg
    12421 tccctcctcc ttttaatgca taatttctat tgaaaataaa tcaactctca tctcccccat
    12481 ctcggctgcc agtctgacgt taggctgttt gtaatgcgag ttaaccctgt tccattgggg
    12541 cggatcttat aaatttcatt gttcccatca cgaaaggaag tgaaggcgat ccattcacca
    12601 tccggtgacc agctgggacc cagattatcc ccaccattgg tgagtctctc cagccctgat
    12661 ccatcgatct taatgacgta gatatcgcgg tcgccctcag gtccgcgata aaaagcaagc
    12721 cactttccat tcggggacca ggtgctgcga ccacccatat tggtgaggtc agtcacctgg
    12781 ttcacttgct ttccacttgc cttcgcagta tataactgtc gggttccaga acggtttgag
    12841 gcaaaagcga tcattgagcc atcgggtgac caggtggggt cgatatcatc acgaaacgtg
    12901 atcgcatgtg ggtcactgcc atccgcgttc atcacccata ccccattccc attgctggta
    12961 aagataattt tgctcccatc gggggaaagc tcgggcgcat ataacgtgcc gatgttttgt
    13021 gtaaggcgct gcaggttatt gccattgaca tcaatggaat aaatctcata ggatccgcta
    13081 tgtcgcgaag agaagtatat ggtctgacca tccggtgaaa gcgaggcata aaaggcagtc
    13141 gccgagaggt ttgtgagttg tctgcgtccg gtaccatccg cattcacaat gcagatctgg
    13201 tcaatctgtt cgatgaaaca cgtgaataca atcttgccac cgagtgaagt gccaggaacg
    13261 ggagaaggaa tcaaactagg aacaatggtc ggagataacg ttggggtaga gaggtcaaga
    13321 aacggcgtcg agacaacagg tgaagctgaa aagtcaatct gcgtcggttc tacgcgtgag
    13381 acgagtggat tctgccagat gtaaacaaat gcgcccgcga gaatacaaag cccaagcacc
    13441 ccacagccta aaccaccgaa aaggagggca tcaaagatcg gaagaatttt tttgggagat
    13501 tccatcagtt cagttaatat taaaacggaa cctgtagatg caatgttatt acacccagct
    13561 cacatgcatc ttgacgaatt gcaaactatc tcccccaccg aggctgccaa tcagagattg
    13621 cgctgttcgt cacgcgcgtc gcaccagttc catccgggcg gatgatatag atttcgttat
    13681 tcccatctcg aaacgaagta aaggtgatcc aatttccatc gggcgaccag ctgggtccga
    13741 ggttatcgcc accattggtg agctgcacca atccggtgcc atctgcattg atgacgtaga
    13801 tgttgtgatt gccggcaggt ccggcataaa atgccaattg ccgtccgttg ggagaccagg
    13861 tactgcgacc acccatatcg ggtagattcg tcaactggcg gatctccgtc ccgtcacgtt
    13921 ccatcacaaa taactgccgc tgacctgtcc gcgaggaagc aaacgcgatg tacgcgccat
    13981 ccggcgacca ggtggggtca atatcatcat acggaccatt cgtgatgggg tgcggattat
    14041 tgccgtctgc gcgcatggtc cagatttttt gac
//
LOCUS       DASTJP010000572         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_775921, whole genome shotgun sequence.
ACCESSION   DASTJP010000572 DASTJP010000000
VERSION     DASTJP010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775921"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..2344
                     /locus_tag="VFQ23_16790"
     CDS             179..2344
                     /locus_tag="VFQ23_16790"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HET9908307.1"
                     /translation="MQNRNNKFILPGILGALAFCAFCVCCGAVAVYLYLTPINQTVSP
                     ISTDSNVPVNPTPQISVDPSGLAEWTVIVYSAADDEVLEETMWFDVNEMELVGSNPQL
                     NIVVQIDRYGGAFDGDGDWTDARRYLVTRDNDLSTISSPIVQSLGEVDTGNPQTLVDF
                     VTWAIQSYPARKYALVLSDHGGGWTGGFSDMTSPNSDLSMPEIVSAIETIQQNTGVDK
                     FEMIGFDACLMGQIEIFGALYPYSNYMVASEEVIPGYGWSYAAWLGQVAQNPAMDGNG
                     LSNSIVSTYVVNDVLLTDELRASADEIAQEESTTTLSAIESARVPDVIGAMNQFIESM
                     AALDQGIVAEARTYARSYFSLFGEEVSPSFIDLGNFSEVLASLTDDPGAQQAAVQLQS
                     AIDSAVVAEKHGSSVSGSNGIAFHFPDSDLYYFTEYNGDTPTYADSSSKFLEQSSWDE
                     FLAYHYTGAEFVPQEGQAVTPSRTAEIIAPGASELTIGPIQISDTDISGDEIVTVSTT
                     VSGNVAYIHTALYFWDPATESYWIADVSYYIAENTISIDGVNSPDYGESPIQVEYEWS
                     PTLYSLTDGTHDAFVLLEPAEYLSTDGETVYYVYGQYTAAVSNTPVDAGLYFDADGNF
                     LYAYAFPDSNNDGASNPVEITPQVGDTFTDYVQYYEYDANDNPIYDYVLSDDVFTWGE
                     QGFSFYSSYPVDGQYSVGIIAYDFDNNFVANFGLIDYTR"
     gene            complement(2349..2837)
                     /locus_tag="VFQ23_16795"
     CDS             complement(2349..2837)
                     /locus_tag="VFQ23_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9908308.1"
                     /translation="MASFEDYKYCPRCTTPVIHENRHGAIRAVCPNCKWIHFSDPKVA
                     AAVLVEQNGQVLLVRRVGEPFRGFWTLPAGFINSGEDPAEAAARECLEETGLRVRVTG
                     IYEIVAGREHPSGADFVIVYHAEVIDGELSPSDDADAAEWFPRGNLPPLAFQATKKVL
                     LG"
BASE COUNT          688 a          723 c          723 g          759 t
ORIGIN      
        1 tcaaccataa aattattcta ccgcttgtct cttaataagt aaacttttcg tatggtttgt
       61 aaatcaattg agtgccgggg tttactcaag aagctttgaa acgattctaa aacttgactt
      121 gtgatttgcc tccatgctac actcgccgag cttacatcat ccctttcagg agccagccat
      181 gcaaaataga aacaacaaat tcattcttcc gggcatcctg ggtgcacttg ccttttgcgc
      241 gttttgcgtt tgctgtggcg ctgtagcggt ctatctttat ttgaccccta ttaatcaaac
      301 cgtttctccc atctcgacag atagtaatgt gccggttaat cccacgccac agatatccgt
      361 ggacccttcc gggttggcag aatggactgt catcgtttat tccgcggcgg atgatgaagt
      421 gctggaagag acgatgtggt ttgacgtcaa tgagatggag ctggttgggt cgaatccgca
      481 actgaatatt gtcgttcaaa ttgaccggta tggtggagca ttcgatggcg atggggactg
      541 gacggacgct cgcagatatc tcgttaccag ggataacgac ctaagtacga tctcttcccc
      601 gattgttcaa tctctcggtg aggtagatac aggcaacccg caaacgctgg tggatttcgt
      661 cacttgggcg attcaatcct acccagcccg gaaatatgca ctggtgctat ctgaccatgg
      721 cggcggctgg accggagggt tttcagatat gacctcgccg aattcagatc tttccatgcc
      781 cgagattgtc tctgcaattg agacgataca gcaaaatacc ggcgttgata aattcgagat
      841 gatcggattt gatgcctgtt tgatgggaca gattgaaatc tttggcgcgc tgtaccccta
      901 ttcaaattat atggttgcct cagaggaggt tatccccggg tatggatggt cctatgcggc
      961 ctggcttggg caggtggcgc aaaaccctgc tatggatgga aatggactct ccaactcgat
     1021 tgtttccact tatgtggtta atgatgtctt actgactgat gagttgagag catctgctga
     1081 cgaaattgca caggaggaat ccaccaccac cctcagtgca attgaaagcg ctcgcgttcc
     1141 ggatgtgatc ggtgccatga accagtttat cgagtcgatg gctgcgctgg atcaaggtat
     1201 tgtggcagaa gcccgcacct atgcgcgcag ctatttctcg ttgtttggtg aggaggtctc
     1261 cccctccttt atagaccttg ggaatttttc tgaggtcctt gcaagcctga ccgatgatcc
     1321 gggtgctcaa caggcggcgg ttcaactaca aagtgcaatt gactcagccg ttgtggcaga
     1381 gaagcatggg tcgagtgtct ctgggtcaaa tgggattgct tttcactttc ctgactcgga
     1441 tctgtattat ttcacagagt acaacggcga cacccccact tatgccgact cgtcctccaa
     1501 attccttgag cagtcctcct gggatgaatt cctggcttac cactacaccg gcgcggaatt
     1561 cgtgccacaa gaagggcaag ccgtcacacc ctcccgcact gccgagatca tcgcgcccgg
     1621 cgcctctgaa ttaaccatcg gaccgattca gatttctgat accgatatca gcggcgacga
     1681 aattgtaacc gtatctacta cagtttctgg caacgttgcc tatattcaca cggcactcta
     1741 tttttgggat cccgcgaccg agtcctattg gatcgcagac gtgtcctact atatcgccga
     1801 gaatactatc tcgatcgatg gcgtcaactc tccggattat ggcgaaagcc caatccaggt
     1861 ggaatacgag tggtcgccca cactatatag cctcaccgat ggaacacatg atgcctttgt
     1921 gctattggag ccggcagagt atttaagtac tgatggagag acggtctatt acgtctatgg
     1981 acagtatacg gctgctgtca gcaatacacc cgtggatgcc gggttgtact ttgatgctga
     2041 tgggaatttc ctttacgcgt atgccttccc ggatagcaac aatgacggag ccagcaaccc
     2101 ggttgagatc actccacagg ttggcgatac attcaccgac tatgttcaat actacgaata
     2161 cgatgccaac gataatccga tttacgatta tgtgctgagc gatgacgtgt ttacctgggg
     2221 cgaacagggt ttctcgtttt attcaagcta cccggtggat ggtcaatact cggttggcat
     2281 cattgcttat gattttgaca ataactttgt cgcaaatttt ggactgatcg actatacgcg
     2341 ctgatcgatc agccgagcag tacctttttt gtagcttgaa acgccagcgg aggtaaattg
     2401 ccgcgcggga accattcagc agcatcggca tcatcacttg ggcttaattc accatcgatg
     2461 acctctgcat ggtagacaat aacaaagtcc gcgccgcttg ggtgttcacg acccgcaacg
     2521 atttcataaa taccggtcac gcggactctc aagcctgttt cctctaagca ttcgcgtgcg
     2581 gccgcttcgg ccgggtcttc gccgctgttg ataaagccgg caggcagggt ccaaaaccca
     2641 cgaaagggtt cacccacgcg gcgcacgagg agaacctgtc cattttgttc gacgagaacc
     2701 gcagcagcca ctttggggtc agaaaaatga atccatttac agtttgggca cactgctcga
     2761 atagctccat gacgattttc atggatgaca ggcgtggtgc aacgcgggca atatttataa
     2821 tcttcaaagc tagccatgcg ggatattatg acacaaaaaa ggtgacagtc acttttgcga
     2881 agcatccccg aag
//
LOCUS       DASTJP010000573         8723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1057612, whole genome shotgun sequence.
ACCESSION   DASTJP010000573 DASTJP010000000
VERSION     DASTJP010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057612"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFQ23_16800"
     CDS             complement(<1..474)
                     /locus_tag="VFQ23_16800"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET9908309.1"
                     /translation="MSQELIIVIALALIFDVLNGMRDASNIVATMISSRAFGPRTALG
                     IAAIAEFLGPFLFGVVVAKTIGDEIVQSSVLTLEVIAASLVGAIAWNLITWFFGIPGS
                     SSHALIGGMVGAVLASAGMDAVKLAGLNKVLIALFASPLIGFILGFILTRIIYFLV"
     gene            complement(571..1191)
                     /locus_tag="VFQ23_16805"
     CDS             complement(571..1191)
                     /locus_tag="VFQ23_16805"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET9908310.1"
                     /translation="MFGLFKKREDVFHKLIQEQASITFEGLKLLVKYLEKQSPEISEE
                     LTMKEKEADEVRRILIDELNRSFVTPFDREDIFALSRSIDDVIDYADSTVSEMEVLKV
                     DPTPFMRRIASLLKDAAYEIFQAVQRLQKNPAVATEHAQRAKTLENRVEGVYREAIAD
                     LFKGPEDVHHVVEMLKLREVYRHLSNAADRGDEAANIIADIVVKKM"
     gene            complement(1319..1744)
                     /locus_tag="VFQ23_16810"
     CDS             complement(1319..1744)
                     /locus_tag="VFQ23_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9908311.1"
                     /translation="MTTDVETICPDDTLQTAAQKMRDRDIGFLPVCDGDRLIGVLSDR
                     DVIVRALADGMDSSAIVGRDLITSPAIYCFDDQGLDEAAKLMHDNQIRRLVVLDRDNK
                     QMVGVISLGDLAINVDDKLSVEVLQSVSEPESINRPEPG"
     gene            1768..2061
                     /locus_tag="VFQ23_16815"
     CDS             1768..2061
                     /locus_tag="VFQ23_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908312.1"
                     /translation="MPFFDAVFQLVDAMLGGAVRTTIENSIGLHAMPDDPTGTVGTGR
                     SQCVDGAFKAVEYMRFTAHAHFKTFIVRVAAYFTSQIAVFHCVLISIHDLPLS"
     gene            complement(2092..2502)
                     /locus_tag="VFQ23_16820"
     CDS             complement(2092..2502)
                     /locus_tag="VFQ23_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908313.1"
                     /translation="MPVRRQRKSDQVNTKKTKPKTKPKKQLKRKAPGSGGIGEYYHVE
                     LRPGEEFVTFQTQDVGRRGHIQRVAGRRSSGYWTTVKWLIGKEDAHIQDERLVPDSKA
                     AKDVIQGLGSQPVRLIGDRFRARPKPNVSESLKP"
     gene            2736..5021
                     /gene="recJ"
                     /locus_tag="VFQ23_16825"
     CDS             2736..5021
                     /gene="recJ"
                     /locus_tag="VFQ23_16825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET9908314.1"
                     /translation="MTIWRDPEPVDISILNSLRLHPLVAQTLIRRGITTPGSASAFLN
                     LGSTSPMSFPNIENAAGRIRSAIKNDERIGVWGDFDVDGQTATTVLVQTLQALGADVI
                     YYIPIRGKEGHGIHIESLEPMFDQGVKLLLTCDTGITAHKAIDYANSRGADVIITDHH
                     DLSETLPNAAAIINPKLLPADHPLATLAGVGVAYKLAEALLIENRNSETANLLDLVAL
                     GLIADVALLRGETRLLVQQGIQELRQTNRLGLKTMAELSGTSLETLTEETIGFSFAPR
                     LNALGRLSDANPAVELLMTNDPARARVLATQVEGLNAQRRLLTSQVYEAAEAKLHAKP
                     DWLTEPVIILSHPNWPGGVVGIVANRLVDRYHKPVILLTESEDGILRGSARSIEGLHI
                     TQAITTQKDSLLSFGGHPMAAGLALQRDKLGEFKKGLGRAIERQLGTATRQEPVLQID
                     AWLGLEELHLELANALESLAPFGAGNPPLIIASRAVQLKSVTTMGKAKEHLRITVEDG
                     NGNAQSLLWWGGAGNDLPEEGSIIDLAYSLRANTFRGKRQVTIQFEDFRVVEENIALN
                     TSRIEVEDWRLQTAKFNELNESVLVWAEAADKSKGKNRFELHPAEELAIYTTPASPAD
                     LGLALDIVKPKKIYLFAVTPSTQGNSGSSKTTDEFLSRLAGLAKYAINQRGGKVAIQA
                     LAAATGQRESAVQIGLEWLAAGGHISIQAQEDALQVSNGTGESNQYLQKELYIAVKGI
                     LEETLAYRAHFARATVETLIQ"
     gene            complement(5025..5804)
                     /locus_tag="VFQ23_16830"
     CDS             complement(5025..5804)
                     /locus_tag="VFQ23_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908315.1"
                     /translation="MDNDNPPAFDRSLLPPALIGIFSVIGICLVLLVYRYNTSSTVIE
                     VTVTHTPFTYIFLGTEPSISTEPSPEETVTATLEEEPPLEFSPEPPVLLTPNTPQVIV
                     IPTTSIPSTVIGPNATFTLTSASTPPLNPGTYDDIDFRLVYSGNWVNQTNVGGAYLNT
                     LHISNTLTSPGNSVTFRFIGQEVRILYQSGGSLGVLRITLDTTTTQLDQSDSSNELVL
                     TATQAGTHTVTLTHFSGGSVNLDSVIVPSISTATPTPTATP"
     gene            5901..7343
                     /locus_tag="VFQ23_16835"
     CDS             5901..7343
                     /locus_tag="VFQ23_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET9908316.1"
                     /translation="MLNIIPKPASVLESGGNLVLSTNTIIVLQNEAPEISFIANLLSS
                     FVKEVSGYEVPVLEQSDTSSQTCIYLSINADESLGEEGYDLSITNDGIRLRANCPAGL
                     FYGVQTLQQLFPAHSARSLSLPMVSIRDVPRFEWRGAMLDVARHFFSVEDVKRYIDLI
                     SHYKVNRLHMHLSDDQGWRIEIKSWPRLTEVGSSTQVGGGEGGFYTQEQYKEIVRYAR
                     SRYVTVVPEIDTPGHTNAALASYAELNSSEEAPELYTGMQVGFSTLWIKSEITYKFLN
                     DVIGEIAALTPTPYLHIGGDEARATPEEDYKYFIKRIQEIVVSHDKTMIGWNEIGEAE
                     LLPGTIVQQWVGAGYQNAKKQGAKFILSPADKTYLDMKYDESTRLGQDWAGLISVKHA
                     YDWEPGSSVDGLEESDVLGLEAPLWTETIQTMKDIEYLTFPRLLGIAELAWSPKGQSW
                     EEYRRRLAAHGKRMEAMGINFFKSPDVDWE"
     gene            complement(7383..>8723)
                     /locus_tag="VFQ23_16840"
     CDS             complement(7383..>8723)
                     /locus_tag="VFQ23_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9908317.1"
                     /translation="ADDEILSTTKEHVFFTWSAQSKVNPIALKRAQGVYFWDVDDKKY
                     LDFNSMTMCVNIGHGDERVIGAMQEQAAELAYAAPGMTTKVRALASKAVADITPRQAL
                     TKILFTLGGADANENAIKLARGYTGRHKILARYRSYHGATAGAMAATGDPRRVVWEPN
                     LMSGVVHFLDPYRYRSTFHRTNPDISEKEFAQDYLNHLEEIIRYEGPQTIAAILMESV
                     TGANGVIIPPDGYMQGVRALCDKYGIVMIADEVMSGFGRTGKWFAIEHWDVVPDMITM
                     AKGLTSAYAPLGAVAMKPEIAAAFNETVYESGLTYTSHPISLAAAIANIEVMREDKLV
                     EHAAGMGKVLKRMLTDLGEAHPSVGEVRNIGLFGILELVKDRKTKEPMAPWNESSAEM
                     TALKRYCLDHGLFLYTHWHTALIIPPLIITEEQLKEGFDILDKALEIPDKSTRP"
BASE COUNT         2237 a         2202 c         2149 g         2135 t
ORIGIN      
        1 caccagaaaa taaataatgc gcgtgagaat aaacccaagt ataaaaccga tcaatggcga
       61 tgcaaacaga gcgatcaaca ccttattcaa acctgcaagc ttgaccgcat ccattcctgc
      121 gcttgctaac acggcgccca ccattccgcc gatcagcgca tgagaggaac tacccggaat
      181 gccaaagaac cacgtaatta gattccatgc aatggcaccc accagagacg ctgcaatcac
      241 ctccagagtc agcacgctgg attgaacaat ttcatcccca atcgtttttg ccacaaccac
      301 accaaacaaa aacggaccga gaaattccgc aatggcggca atgcccagcg cagtccgtgg
      361 accaaacgca cgcgaggaaa tcatcgtcgc aacgatattg ctcgcatcgc gcatcccgtt
      421 cagtacatca aaaattaatg ccagggcaat aacaataatc agttcttggg acataaaatt
      481 tgcaagtgtc attgcgaggg tgatagcccg aagcaatctc ctgattgacc aggcgactgc
      541 ttcgccaaag gctcgcagtg acgtaatcaa ctacatcttc ttcaccacaa tatctgcgat
      601 aatattagcc gcctcatcgc cacgatcggc tgcattcgac aggtgccgat atacctcacg
      661 caacttcaac atttcaacaa catgatgtac atcttccgga cccttgaata ggtcggcgat
      721 tgcctcgcga tatacacctt ccacacggtt ttcgagagtc tttgcccgct gggcatgttc
      781 agttgccact gccgggttct tttgcaaacg ctgaacggcc tgaaagattt catacgccgc
      841 atccttgagc agcgatgcga ttcgcctcat gaacggagtg gggtcaactt tcagcacttc
      901 catttcgctg acggtgctgt cggcataatc aataacatcg tcaatggaac gtgacagggc
      961 aaagatatcc tcccggtcaa agggcgtgac aaacgagcga ttcagctcgt caatcagaat
     1021 ccgacgcacc tcgtcggctt ctttctcttt cattgtaagt tcttccgaaa tctcaggaga
     1081 ttgtttctcc agatacttga ccagcagttt aagtccctca aacgtaatgg aagcttgttc
     1141 ctgaatcagt ttatgaaata cgtcttctcg ctttttaaat aatccaaaca taattacctc
     1201 gggaaatatt ggacctgctg taatgagagg caatcatatc actagaaatt agcaatctaa
     1261 ttaaaagtca ataaagctaa atcaatagct aaggtcgacc cgttaaattt atctggtcct
     1321 acccgggctc tggtctgttt atgctttcag gttccgaaac actttgcagc acttcaacag
     1381 ataacttatc gtcaacattg atcgccaggt cacccaatga aatgactcca accatctgct
     1441 tgttatcccg atccagaacg acaagccgac ggatctggtt atcatgcatc agcttggcag
     1501 cttcatccaa tccctggtca tcaaaacagt aaatcgcagg ggaagtaatc aggtcccgcc
     1561 caacaattgc gcttgaatcc ataccgtcag ccaatgcgcg gacaatcacg tcccgatcgc
     1621 tcaaaacacc gatcaagcgg tcgccatcac agaccggcaa aaacccgatg tcgcggtcgc
     1681 gcattttctg tgcggcggtt tgtaacgtat cgtctggaca aattgtctcc acgtccgttg
     1741 tcatgatctc atgcagtttc atgctggatg ccgttcttcg acgcggtctt ccagctggtg
     1801 gatgccatgc tgggaggcgc agtgcgcaca acaatagaaa actccatcgg cctccatgcc
     1861 atgcccgatg atcctacagg cacagtgggc acaggtcgga gccaatgcgt ggatggcgca
     1921 ttcaaagctg tcgaatatat gcgtttcacc gcccatgcgc acttcaaaac ttttatcgta
     1981 cgtgttgccg catacttcac aagtcagatt gctgttttcc attgtgttct tatctctatt
     2041 catgatttgc ctctttcgta actaaattgg tatttaccag cactgtttat attaaggctt
     2101 aagggattcc gaaacattcg gtttcggtct ggctcggaac cgatcgccga tcaaacggac
     2161 gggttgtgag ccaagcccct ggataacatc cttggcagct ttggagtcag gaacgagcct
     2221 ttcatcctga atgtgggcat cttccttgcc aatcagccac ttgactgtcg tccagtagcc
     2281 actcgacctt ctaccggcca ctctttgaat atgaccacgt cgccccacat cctgcgtttg
     2341 aaaggtaaca aattcttctc ccggccgcag ctccacgtga taatactccc ctatgccacc
     2401 actgcccggc gctttccgtt tgagttgttt cttcggtttc gtctttggtt tcgtcttttt
     2461 cgtatttact tggtctgatt tgcgttggcg cctcactggc ataataaaac tccttatcca
     2521 gtagtcaaaa tttgaatcga tcgagccgtt tccttatcta aaagcatgct acactccgat
     2581 aaagcgagcg ataggctcta ttgaataagt attatgttaa tgacccgaat tatcgtcaat
     2641 acggtcttgc ctttatcccg gtagggaaat ccctaccccg attgggatgt agaataagtt
     2701 ttgtggcgaa ttgacttata agagagcaaa tatccatgac aatttggcgt gatcctgaac
     2761 cagttgacat ttctattctc aacagccttc ggttgcaccc gctggtcgca caaacgctga
     2821 ttcgacgggg cattactaca cccggatctg ccagcgcatt cttaaatctg ggttcaacct
     2881 cgcccatgtc gtttccaaat attgaaaacg cggcaggtcg aatccgctcc gccataaaaa
     2941 atgatgagcg aatcggcgtc tggggagact tcgacgtgga cggacaaacg gctaccacgg
     3001 ttcttgttca gacgctgcaa gcgctcggag ctgatgttat ttactacata ccgatccgcg
     3061 gcaaagaagg tcacggaatt catatcgagt ctctggaacc tatgttcgat cagggtgtga
     3121 agctgctgct tacctgtgat accggtatta cagcgcataa agctattgac tatgccaact
     3181 cacgcggcgc agatgtcatc attaccgacc accacgattt gagtgaaact ctccccaacg
     3241 cggccgctat catcaaccca aaattattgc ccgcggatca tccccttgct acgcttgcag
     3301 gtgttggcgt agcctataaa ttggcagaag ctctcttaat cgaaaatcga aattctgaaa
     3361 cagccaatct cctcgatctt gtcgccttgg ggcttattgc tgatgtagcc cttctaagag
     3421 gagaaactcg cctgctcgtc cagcagggga ttcaggaatt gcgacaaaca aatcgtctcg
     3481 gcttgaaaac aatggcagag ctttcaggga caagtcttga aacactcacg gaagaaacca
     3541 ttggcttttc gttcgcaccg cgcttgaacg ctctggggag actaagcgat gcaaatcctg
     3601 cggttgaatt actgatgaca aacgacccgg cgcgggcgcg tgtactggca actcaggtgg
     3661 aagggctcaa tgcacagcgc cgtttattga caagccaggt gtatgaagcc gcggaggcaa
     3721 aactacatgc aaagccagat tggctcaccg agccggtaat catcctgtcg catccaaact
     3781 ggcccggcgg cgttgtgggc atcgtcgcca accgattggt ggaccgctat cataaacccg
     3841 tgatcctgct gaccgagtct gaagatggca ttctacgcgg ctcggcacgt tcgattgaag
     3901 gtctgcatat cacgcaggcg attacaacgc aaaaagatag tttgctgagc tttggtggtc
     3961 atccgatggc ggcgggcttg gcgttgcaaa gggacaaact gggcgaattc aaaaaaggac
     4021 ttggcagggc aatcgaaagg cagcttggca cagctacccg tcaggaacca gtattacaga
     4081 tcgatgcctg gctaggttta gaagaactcc atcttgagct tgccaatgcc cttgaatcac
     4141 tggccccctt tggcgcaggg aatcctccgc tgatcatcgc atcccgggcg gtgcagctga
     4201 aatctgttac tacaatgggc aaagcaaaag agcatcttcg tatcacggtt gaagacggaa
     4261 acggaaatgc acaaagcctg ttgtggtggg gcggcgcggg aaatgacctt cctgaagaag
     4321 ggagcattat cgatctcgca tattcgttgc gggccaacac ctttcgcggc aaacgacagg
     4381 tgacgattca atttgaagac tttcgcgtgg tcgaggagaa catcgctctc aacacatcaa
     4441 ggattgaagt ggaggattgg aggcttcaaa ctgcaaagtt caacgaactt aacgagagtg
     4501 tactggtttg ggcagaggcg gcagacaagt caaagggcaa aaatcgtttt gagttgcacc
     4561 cggcggaaga acttgcaatc tacacgacac ctgcctcgcc agcagacctg ggtttggcat
     4621 tagatattgt gaaaccgaag aagatttatt tattcgcagt tacaccgagc acacaaggca
     4681 attccggttc ttcgaaaacc acagacgaat tcttatcgcg cctggctggc ttggcaaaat
     4741 atgccatcaa tcaacgcggc ggcaaagtag cgattcaagc gctggcggca gcaaccggtc
     4801 aacgtgaaag tgccgttcaa attggattgg aatggctggc agccggaggg catatatcta
     4861 ttcaagcgca ggaggacgcg ctacaagtat ccaatggaac tggagaatcg aatcaatatc
     4921 tgcaaaagga gttgtacatt gcagtcaagg gaatcctcga agaaacactt gcttatcgcg
     4981 ctcactttgc gcgggcaact gtggaaacat taattcaata agatttacgg agttgcagtt
     5041 ggcgtcggag tagctgtgct gatactgggc acaatgacgg agtccagatt aaccgagcca
     5101 ccgctgaagt gtgtgagggt gactgtatga gtgccggcct gagtcgcggt taagaccaac
     5161 tcattcgatg aatcggactg atccagttga gtggtcgttg tatccagggt gatccgcagc
     5221 acgcccaggc tccctccaga ctgatataga atacgcacct cctgaccgat aaaccgaaac
     5281 gttactgaat ttccggggga cgtaagcgta ttggaaatat gcagcgtatt taggtaagca
     5341 ccgccgacat ttgtctggtt gacccaattg ccgctgtaaa cgagccgaaa atctatgtcg
     5401 tcatacgtgc cagggttaag tggtggtgta gacgcggagg tcagtgtgaa cgtggcgttt
     5461 ggcccgatca ccgtcgaagg gattgatgtg gtgggaatca caataacctg cggtgtattc
     5521 ggtgtcagga ggaccggagg ttccggtgaa aattccaggg gtggttcctc ttcaagcgtt
     5581 gcggtcacag tttcttcggg ggatggctca gtcgaaatag atggttccgt tcccaaaaaa
     5641 atgtacgtga acggcgtgtg ggttacggtc acttcaatca ccgtgcttga ggtgttgtac
     5701 cgatacacca gcaggacaag gcaaattccg atgacggaga agatgccgat caaggctgga
     5761 ggaagcaggc tacggtcgaa cgctggcggg ttgtcgttgt ccatggaaat gaggaatcag
     5821 tggcgggttg agatgagtcc acttacaagt tgactctcaa atgagacaag cggtatcatc
     5881 ggttggagga taccacaatc atgcttaata tcataccgaa gcccgcatct gttttagagt
     5941 ctggagggaa tcttgttctt tcaacaaata cgatcatcgt tttacaaaat gaagcccctg
     6001 agatttcttt tatagcgaat cttctttcgt cttttgtgaa agaagtaagt ggctatgaag
     6061 ttcctgtatt agagcaaagc gatacgtcat ctcaaacttg catttacctt tctatcaatg
     6121 cggatgaaag cctgggggaa gaagggtacg acctatcgat tactaatgat ggcatcagac
     6181 tccgcgcaaa ctgcccagcg ggattatttt acggtgttca gactcttcaa caacttttcc
     6241 cggcccactc tgcccggagc ttgagcctgc ccatggtttc gatacgcgac gttcctcgct
     6301 ttgaatggcg cggcgcaatg ctcgacgtcg cgcggcattt ctttagcgtg gaagatgtga
     6361 agcgttatat cgacctgatc tctcactata aggtcaaccg tttgcacatg catctttcag
     6421 acgatcaggg ttggcgcatc gaaatcaaat cgtggccacg cctgacggaa gttggcagca
     6481 gcacccaggt cggaggtgga gaaggggggt tttacaccca ggaacaatac aaagagattg
     6541 tgaggtacgc gcgcagtcgt tacgtgacgg ttgtgccaga gatcgataca cccggtcaca
     6601 caaacgccgc gctggcatcc tatgcagagc ttaatagttc ggaggaagca cctgaattat
     6661 atacaggcat gcaggttggc tttagcacct tgtggataaa aagcgaaatc acatataaat
     6721 ttttgaatga tgtcattggt gagatcgcgg cgctcacacc gacgccctac cttcacattg
     6781 gcggtgacga agcgcgcgcc acgcccgaag aggattacaa atacttcatc aaacggattc
     6841 aagagattgt cgtttcacac gacaaaacca tgatcggttg gaatgaaatc ggggaggcgg
     6901 aactgctgcc gggaacgatt gttcaacagt gggttggcgc tgggtatcag aatgcgaaaa
     6961 aacagggtgc aaagtttatt ctttccccgg cagacaaaac ttatcttgat atgaaatatg
     7021 acgaatctac tcggcttgga caggactggg caggcttgat cagcgtaaaa cacgcctatg
     7081 attgggaacc tggctcttct gtcgatggac tcgaagaaag cgatgttctg ggtctcgaag
     7141 caccattgtg gacagaaacg attcagacca tgaaagatat cgaatacctg accttcccgc
     7201 ggcttctcgg tattgccgaa ttagcctggt cgccaaaagg tcagagctgg gaagaatatc
     7261 gccggcgcct tgcagcacac gggaagcgca tggaagcaat gggaattaat tttttcaaat
     7321 cgcctgacgt ggattgggaa taattaacaa cgtagggatg agccgttgct catccctacg
     7381 ttttacggtc ttgttgattt atccggaatt tccagcgctt tatccagaat atcaaatccc
     7441 tctttcaatt gttcttccgt aataatcagg ggtgggatga ttaaggcggt gtgccagtga
     7501 gtatataaga acaatccgtg gtccaggcaa tatcttttta gcgcagtcat ttctgctgac
     7561 gactcattcc atggggccat cggttctttt gtttttcggt ctttaaccag ttcaaggatt
     7621 ccaaacaagc cgatgttccg aacttcgccc accgatggat gcgcctcgcc gagatcggtc
     7681 aacatgcgct tgagcacttt ccccatccct gctgcgtgct cgacaagctt gtcttcacgc
     7741 atcacctcaa tattcgcgat cgccgcagca agggaaatcg ggtgagatgt gtaggtcaag
     7801 ccactttcat agacagtctc attaaacgct gccgcaatct ctggcttcat cgcgaccgct
     7861 ccaagcggcg cgtaggcgga ggtaagtccc tttgccatcg tgatcatatc gggaaccaca
     7921 tcccaatgtt cgatggcaaa ccatttgcct gttcgaccaa atccgctcat gacttcgtcg
     7981 gcgatcatca cgattccgta cttatcgcaa agagcacgta cgccctgcat gtatccgtca
     8041 ggcgggatga tgacaccgtt cgccccagtg accgattcca tcaggatcgc tgcaatggtc
     8101 tgtggtccct catatcgaat aatttcttct agatgattga gataatcctg cgcgaactct
     8161 ttttctgaaa tgtcaggatt tgttctatga aatgtggagc gatagcggta cgggtcaaga
     8221 aaatgcacaa caccgctcat caaatttggc tcccagacca cgcgacgcgg gtcaccggtg
     8281 gcggccatcg ccccggccgt cgcgccatga taagagcgat agcgcgccaa aatcttatgt
     8341 ctgcctgtat aaccgcgcgc cagcttgatc gcgttttcgt tggcatctgc tcccccaagt
     8401 gtgaacagga tcttcgtcag ggcttgacgc ggtgtgatat ccgctactgc tttgctggca
     8461 agcgcacgaa ctttggttgt catgccgggc gcagcatagg caagttcggc cgcctgctcc
     8521 tgcatggctc cgatgacccg ttcatcccca tgaccaatat tgacacacat ggtcatggag
     8581 ttgaaatcaa gatatttttt gtcatcaaca tcccaaaaat aaacaccctg ggcgcgcttc
     8641 aaggcaattg ggtttacctt ggactgggcg gaccaggtaa aaaatacgtg ttcctttgtt
     8701 gtgctcagaa tttcatcatc ggc
//
LOCUS       DASTJP010000574         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1490628, whole genome shotgun sequence.
ACCESSION   DASTJP010000574 DASTJP010000000
VERSION     DASTJP010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490628"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1390)
                     /locus_tag="VFQ23_16845"
     CDS             complement(125..1390)
                     /locus_tag="VFQ23_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908318.1"
                     /translation="MSFPDIRRSRRWLSVEELRILILLFILLIGLLALNIYLARSLPG
                     GEWFYLRWSGARAFLFENVEPYSRTIAERVQELIYGRIATSTEYPYVLNDPFFIVLLY
                     TPIALFPDFSLARGIWMLLSEAALIGTVLFTLSLLEWEPPGWLMISLLGFGLFSFFSL
                     QSLLAASPTIFLIFIYLAILVALRSYSDELAGALLFLVAYQWEVGALFFLYILIMVFA
                     NRRWGVLTGFGMSLFLALVVSFLIYSGWGLPYIRAVLSDWIRGVSLNLNSVLAFWIPN
                     SRFSIAAIVSAVITVVVFIEWIASTRSNFRRILWTSSLSLAATPLVGFAIFPSNHVVL
                     IPALILILMLVWERWIRQRVLAYLLVFSVALLVPFGLYYRSLTVEDRLYSDLLTVLPP
                     VVTILGLYWMRWWVLHSPRTWMDQIGVHR"
     gene            complement(1393..1977)
                     /locus_tag="VFQ23_16850"
     CDS             complement(1393..1977)
                     /locus_tag="VFQ23_16850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908319.1"
                     /translation="MKPLGSKSLTLSFKPLKRNLWPDFEELFGPNGACGGCWCMFWKL
                     RGKAYDEARVYETRQMHKSIVDSGTSTGLLAYYHGEVVGWVAVEPRSDYPKLAHSRAL
                     KPVDDQPVWSVTCFYVSKKFRKKGITVELLKAAIEYVKSNGGKIVEGYPVDAQKDMAA
                     PFVFTGTAAAFKHAGFKEVARHTPTRPIFRFTIK"
     gene            complement(2069..2974)
                     /locus_tag="VFQ23_16855"
     CDS             complement(2069..2974)
                     /locus_tag="VFQ23_16855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9908320.1"
                     /translation="MKIKILLNPYSNRWNSQRRWAEAESALREAGVDFDLSLSEYADH
                     LIDLAAASVKDGFSTLVVAGGDGSIGEVINGAARGWDEKTPFPVTIGVLPLGTANDLA
                     DNIGLPRDLSEAVKVIARGKTRGIDLGKCNERYFVNNSGAGLEPYVTTKQEKIKWISG
                     VPRYLVAAVQGILDKPNWQAKLEWDGGSYEGPLSLASIGNGHRTGGIFFMTPHADPFD
                     GKLTFVHGHRGTRLGLFQALPRAMKPGEGSYVEMDGMYEINCTRLKIHFDKPSPVHTD
                     GELFDKWLTDLEYKLFPNAVQILVP"
     gene            complement(2971..>3284)
                     /locus_tag="VFQ23_16860"
     CDS             complement(2971..>3284)
                     /locus_tag="VFQ23_16860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9908321.1"
                     /translation="EDLSVLSSLPLIGAILYRATLSIGLGYTLQVWGQKHTPPTDAAL
                     ILGLEAVFAVIASWIALGQTLLPIQIFGCVVIFVAVLVSQVKNWNLQGTIDHDRLVEG
                     R"
BASE COUNT          963 a          843 c          794 g          684 t
ORIGIN      
        1 gcgagttgaa taccaccggc gaagtaataa tcggcatcca tatagccggg tagagtctga
       61 aaccgcgaca cgagaaacgg gacaatcaat ccgatgaaaa agagaagcgc gtaatgccat
      121 cgcttcatcg gtggactccg atttgatcca tccatgtgcg aggcgaatgc aaaacccacc
      181 agcgcatcca atagaggccg agaatggtga caacaggcgg aaggacagtc agcaagtctg
      241 aatagagtcg atcttctact gtaagggagc ggtagtatag accaaacgga actaatagag
      301 cgacactgaa aaccagcaaa taggcaagga cgcgttgacg aatccagcgt tcccagacga
      361 gcataagaat caagatcagt gccggaatca agacgacatg attggacggg aagatcgcga
      421 agccaacaag cggcgtggcg gccagcgaca gcgaagacgt ccagagaatt cgccgaaaat
      481 tggaccgggt ggaagcgatc cattcaatga acacaaccac agttatcact gctgaaacaa
      541 ttgctgctat tgagaacctc gaatttggaa tccaaaaggc caaaacgcta ttcaggttca
      601 aacttacacc gcggatccaa tctgacagca ccgcgcgaat gtaaggaagt ccccaaccag
      661 aataaatgag gaacgatacg accagcgcca aaaagagcga cataccaaag ccggtcaata
      721 ctccccagcg tcgattcgcg aagaccataa tcagtatata aaggaagaag agcgcgccca
      781 cttcccattg ataagcaacc agaaagagca gagcgccggc gagttcatcg gaataggaac
      841 gcagcgcaac gagaatagcg agatagataa aaatcaaaaa aattgtaggc gaggcagcaa
      901 gcagagattg aaggctaaaa aaactaaata acccaaaccc cagcagcgaa atcatcagcc
      961 agcctggagg ctcccactcg agtagactca gagtgaacag cactgttcca atcaatgccg
     1021 cttcagacaa gagcatccaa atgcctcgcg caagcgaaaa atctggaaat agcgcaatcg
     1081 gtgtgtaaag cagcacgata aaaaacggat cattcaacac atacggatac tcagtggagg
     1141 ttgcaatccg tccatagatt agctcctgaa ctcgttcagc aatcgtcctg ctatacggct
     1201 cgacattttc aaacagaaac gcccgcgctc cgctccagcg caggtagaac cattccccac
     1261 ccggaagact acgtgccagg taaatgttca gtgccagtaa tccgatgagc aaaatgaaca
     1321 aaaggatgag gatacgcaac tcctccacgc tgagccatct tcttgacctg cgaatatcgg
     1381 ggaaggacat gcttatttaa tagtaaagcg aaaaattggg cgggttggag tgtggcgcgc
     1441 tacttctttg aaacccgcat gcttgaaggc ggccgcggta ccggtaaata caaacggtgc
     1501 tgccatatcc ttttgagcat ccactggata cccttcaaca atttttccac cattggactt
     1561 gacgtactca atcgccgcct tcaacaattc gacagtgatc cccttcttgc gaaatttctt
     1621 tgagacatag aaacaggtca ctgaccagac gggctgatcg tccacgggct tgagggcgcg
     1681 cgaatgagcc agtttgggat agtcactgcg cggctcgacc gccacccaac cgaccacttc
     1741 cccatgatag taagcgagaa gcccggttga cgtacctgaa tccacaatgg acttgtgcat
     1801 ttgccgcgtt tcgtagactc tggcttcatc gtatgccttg ccgcgcagct tccagaacat
     1861 gcaccagcag ccaccgcagg caccatttgg accgaagagt tcttcaaagt cgggccataa
     1921 atttcgtttg agtggtttga aggaaagggt gagagattta gaccctaaag gtttcattga
     1981 taatattaag tccttataca aaaaggtttt ccttgaaaac caagagcttg attgacctga
     2041 aaaaaccttt agggtcggtg tgtgattttc atggaaccag aatttgaact gcattcggga
     2101 atagcttgta ttccaagtcg gtaagccatt tgtcgaacag ttcgccatcg gtgtgaacgg
     2161 gcgaaggttt atcaaaatga atcttcaaac gcgtgcagtt gatttcgtac atcccatcca
     2221 tttctacata actgccttcc cccggtttca tggcgcgagg caatgcctgg aacaatccca
     2281 gtcttgtccc acgatgccca tgcacaaaag ttagttttcc atcaaaggga tccgcatgcg
     2341 gggtcataaa aaagatgcca cccgtgcggt ggccattccc aatggaagca agagagagcg
     2401 gaccctcata agaaccgccg tcccactcaa gcttcgcctg ccagtttggt ttatccagga
     2461 ttccctgaac agccgcgacc aaatagcgcg ggacgcctga aatccatttt attttttcct
     2521 gttttgtggt gacgtagggt tcaagcccgg cccccgaatt attcacgaaa taacgttcgt
     2581 tacacttgcc caaatcgata ccgcgtgtct ttccacgtgc aattactttt accgcctcgg
     2641 acaggtcacg cggcaggcca atgttatccg ctaaatcatt ggcggtgccc aaaggtaaca
     2701 ctcctatggt cacagggaaa ggagttttct catcccagcc gcgcgcagct ccattgatta
     2761 cttcaccaat cgatccgtcg ccgccagcga cgacaagagt agagaagcca tctttaacgg
     2821 aagcagccgc gaggtcaatg agatgatctg cgtattcgga aagggataag tcaaaatcca
     2881 cacctgcttc acgcaaagca gattctgctt cagcccagcg tcgttgagaa ttccagcggt
     2941 tggaataggg attgaggagg attttgatct tcatctgcct tccaccaggc gatcgtggtc
     3001 aattgtaccc tgaaggttcc aattcttcac ttgtgagacc aacaccgcta cgaaaatgac
     3061 aacacagccg aatatctgaa tcggtaaaag ggtttgcccg agcgcaatcc agcttgcgat
     3121 cacagcaaag actgcttcca gaccgagaat gagcgcggca tccgttggag gcgtgtgctt
     3181 ttgcccccat acctgcaggg tgtatccgag tccaatcgaa agagtcgcac gatataagat
     3241 ggcgccgatc aatggcaacg aagataacac agacaagtct tctg
//
LOCUS       DASTJP010000575         6724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_677570, whole genome shotgun sequence.
ACCESSION   DASTJP010000575 DASTJP010000000
VERSION     DASTJP010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677570"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFQ23_16865"
     CDS             <1..432
                     /locus_tag="VFQ23_16865"
                     /inference="COORDINATES: protein motif:HMM:NF015716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HET9908322.1"
                     /translation="PTTENTAQLLGLATRVSKPRPEESRQFAVRDLGDETALFLERTL
                     RSGTRIEFPGHVVVFGDVNPGAEIIAEGNVIIWGRLRGMVHAGSKGNKDAVICALDLS
                     PTQLRIAEEVSAALKPQENPKPEIARINENGKLQAEFWSPG"
     gene            446..1243
                     /gene="minD"
                     /locus_tag="VFQ23_16870"
     CDS             446..1243
                     /gene="minD"
                     /locus_tag="VFQ23_16870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HET9908323.1"
                     /translation="MPAQVITVTSGKGGVGKTTAVANLAVALASTGSKVVCIDGDIGL
                     RNLDVILGLENRIVYDIVDVIEGRCRLKQAMIRDKKLPDLYLIPAAQTRDKNAISPSD
                     MNRLIKELRPDYDYVLIDSPAGIERGFRNAIAAADRVLVVTNPEVSAVRDADRVIGIL
                     EAEEKGPAALIINRLNPTLVKNHDMLSADDVLDLLAVELIGIVPEDENVIVGSNRGAP
                     VAYDQKSKAGQAFRNIARRLKGEKVPFLDLDSGGSLWERLQRLAGRK"
     gene            1245..1523
                     /gene="minE"
                     /locus_tag="VFQ23_16875"
     CDS             1245..1523
                     /gene="minE"
                     /locus_tag="VFQ23_16875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HET9908324.1"
                     /translation="MSFFDRLFGRKNSADSAKERLQLVLIHDRTDLTPAQFDSLRDDL
                     IETISRHVEIDAQSMQIGVEHDGRSQRLVADIPIKSAQRRRARKTTKK"
     gene            1616..2743
                     /locus_tag="VFQ23_16880"
     CDS             1616..2743
                     /locus_tag="VFQ23_16880"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET9908325.1"
                     /translation="MLRDISWRYFDFWLLGTVVLATAFGTTMIRSAVAGNEELLPVIE
                     RQIYFALAGLVLIFVVAAIDYRYWMALYRPIFIVMSILLFSLYLSAQAVFGAARWFQV
                     GVLFVQPTEFAKIAAILLLARYFDKAQNSPRDVRWIIFAFLWTMGLGIWILLQPNLSN
                     VIVLMVILASLLWFNGIQVKHVLLFGGIGVLSLGTIVLLSAFGIRLPFLQEYQQQRIV
                     NFILPDPNATFGATYNVQQALIAIGSGGLFGEGYNSGTQTQLRFLKVRHTDFIFSAIS
                     EEFGLVGSLFVILVIVLVIWRCLRTAQKARDVAGMNIAYGVATLIFFQGMVNIGVNLN
                     IVPVSGLPLPFISYGGSGLTSLMLGIGLVQSVAMRHKQMDF"
     gene            2834..4324
                     /gene="gltX"
                     /locus_tag="VFQ23_16885"
     CDS             2834..4324
                     /gene="gltX"
                     /locus_tag="VFQ23_16885"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET9908326.1"
                     /translation="MTIKPARTRMAPSPTGRFHLGGARTALFDYLLARQTGGQFILRL
                     EDTDQKRFVPGSEEEILRSLDWLGITPDESPAHGGKYGPYRQTERRESYLKHAQILVE
                     KGHAYPCFCSPERLEKMRKEQEVQKVDRVQYDGLCRRIDPDEAARRVANGESYVIRFK
                     MPKEGTTTAHDHLRGDIVTENKYLDDYVILKSDGLPTYHLAAIVDDHEMEITHVLRGS
                     EWLSTFPLHVQIVRAFGWEEPVWIHLSVFLKPSGKGKMSKRESAQAMKDGYSIFVDDM
                     KGLGYIPEGVLNWIALMGWGVAEDELMTLDQMIQRFSIDTLTPSPTAINFQKLDHFNG
                     AHIRLLSTEDLAARIKPYFTDAGLEVRDDMLLKITPLIRERLVTLDDCLAFASFFFKG
                     EVQPTPEDLIAKGLDATGSAEIAKKVYEILNALPDVSHQTAEPPLRSYVESSGLSPNQ
                     VFGILRVAVTGQKVSPPLFESMEVIGREKVLPRLKNAIDLLEKVSH"
     gene            complement(4367..4759)
                     /locus_tag="VFQ23_16890"
     CDS             complement(4367..4759)
                     /locus_tag="VFQ23_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908327.1"
                     /translation="MNKPAFRIHFNDNRQWFNVYVADNPTRFKRRNQCHAYYIAAEVR
                     KQRKGLFGYIYLSELNFSPMAHELVAHEVQHLIFDWVLTRKGMGISEKNEERIATMTG
                     EIARRFWRKYERWSRYNKTPYNRYNRKI"
     gene            5168..5485
                     /locus_tag="VFQ23_16895"
     CDS             5168..5485
                     /locus_tag="VFQ23_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908328.1"
                     /translation="MNRKTTFPMALLLFVLLWGAFGFVAGPSQAQSVAFQTTPVVSTP
                     AVETTLVAPVVTQLVPVTGTTDPSPMMWIFFGLIALLGVVFLVALLAGSGAHRHDGPP
                     PPP"
     gene            complement(5609..6241)
                     /locus_tag="VFQ23_16900"
     CDS             complement(5609..6241)
                     /locus_tag="VFQ23_16900"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9908329.1"
                     /translation="MPSRPVKPAKKFQDLLLSFSQTEELSERKKVEETLWNDYGAEFA
                     VFVLDMSGFSMLTRKYGIVHYLSMVRRMQLTTEPIVKSYGGRMLKYEADNCFAVFPEP
                     LAAVNSAVAMQHAFKASNLLTSDDLDIHISCGIDYGKLLIIGHEDCFGDPVNRASKMG
                     EDVAAAGEILVTKEAMDMIPTDAAIPAHEISISISGVNIPAYVIDYNVME"
     gene            complement(6400..>6724)
                     /locus_tag="VFQ23_16905"
     CDS             complement(6400..>6724)
                     /locus_tag="VFQ23_16905"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF044671.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HET9908330.1"
                     /translation="VKSTIHWVSASHAKAAEIRMYDRLFTKENPEEGDEGFIEALNPN
                     SLTVQTGYVEPLLAQTQPGDRFQFERQGYFATDPDSTAEKPVFNLTVSLKDSWAKIEK
                     SNKSK"
BASE COUNT         1612 a         1569 c         1772 g         1771 t
ORIGIN      
        1 cctaccaccg aaaatacagc tcaattgctt gggcttgcaa cacgcgtgtc caagccacgt
       61 cccgaagagt cacgtcagtt tgcggtacga gatcttggcg atgagaccgc tttattcctt
      121 gagcgcacgc ttcgatctgg aacgagaatc gaatttcccg gtcatgtcgt tgtcttcgga
      181 gatgtcaacc ccggagcaga gattatcgcg gaggggaacg tcatcatttg gggtcgcctg
      241 cgtggaatgg tacatgccgg gtcgaaaggt aacaaagacg ctgtgatctg cgcattggat
      301 ctatcaccga ctcaattgcg cattgcggag gaagtctcgg ctgccttgaa gccgcaggag
      361 aatccgaaac cagagatcgc acggatcaac gagaatggaa aactacaggc agagttttgg
      421 tcgcctggct aaataggata attagatgcc tgcacaagtc attacggtca catcaggaaa
      481 aggtggagtt ggtaagacta cggctgttgc taatctggca gtcgcactgg cctccactgg
      541 ctcaaaagtc gtttgtatcg atggtgacat cggtctgcga aacctggatg tgattcttgg
      601 tctggaaaac cgaatcgttt acgatattgt cgacgtgatc gagggtcgtt gccgcttgaa
      661 acaggccatg attcgagata agaaattacc tgatttatat ctaattcccg cggcacagac
      721 tcgtgacaaa aacgccatct cgcccagtga catgaatcgc ttgatcaaag agttgcgtcc
      781 tgactacgat tatgtgctga tagattcgcc agcagggatc gagcgcggat tcagaaatgc
      841 gattgccgcg gcagatcgcg tcctggtggt tacgaatccg gaggtctccg ctgtacgaga
      901 tgcggatcgc gtgatcggta ttttggaggc agaagaaaag ggtccggccg ccttgatcat
      961 caatcgcctc aacccgaccc tggtgaagaa tcacgatatg ctctctgcgg atgatgttct
     1021 tgatttgctt gcagtagagt tgattggcat tgtgccggaa gatgaaaatg tgattgttgg
     1081 ttcgaaccgt ggggccccag ttgcttatga ccagaagagc aaagccgggc aggctttccg
     1141 aaacattgcc cgccgtctga aaggtgagaa ggttccgttc cttgatctgg acagcggagg
     1201 cagcttatgg gaacgcctcc aacggctggc ggggaggaag taagatgagc ttctttgatc
     1261 gcttgttcgg acgaaaaaac agcgcggata gtgccaagga gaggcttcaa ctggtcctca
     1321 tccatgaccg cacggatctg actcctgccc agttcgactc gttgcgagat gacctgatcg
     1381 agaccatatc acgccatgtt gaaatcgatg cacaatcgat gcagatcggc gtcgaacatg
     1441 atggtcgctc tcagcggctg gtagcggata tccccatcaa aagcgctcaa cgccgccgag
     1501 caagaaagac aaccaaaaaa tagtcttacg ccccaaacga tgctggtcga ctaggtagtg
     1561 atggtttgcc tgttgagacc cggcgtcggc gaaacacact tgtaagaaac cccttatgtt
     1621 acgcgacatc tcatggcgat attttgattt ctggctgctg ggaacagtag tccttgcgac
     1681 tgcattcggc accaccatga tccgatcggc agtggcgggg aatgaagaat tgttaccggt
     1741 gatcgaacgt caaatttact ttgcgcttgc agggctcgtt cttatctttg ttgtcgccgc
     1801 cattgactac cgctattgga tggctttata tcgtcccatt ttcattgtga tgagtatttt
     1861 gttattctcg ttgtacctca gtgcccaggc tgtcttcggt gcggcgcgct ggtttcaggt
     1921 gggcgtgtta ttcgttcaac caacagagtt tgcaaagatc gctgcgatat tgttattggc
     1981 gcgttatttt gacaaagcac agaattcccc tcgagatgtg agatggataa tttttgcctt
     2041 tctatggaca atggggctgg gaatttggat tctgcttcag cctaatttga gcaatgtgat
     2101 cgtgttgatg gtcattcttg cctctttgct ttggttcaat gggatccagg tgaaacacgt
     2161 cctgctcttt ggcgggatcg gtgtactttc gctcggaacg attgtattgc tatctgcctt
     2221 tggaatacgt ctcccgtttt tgcaggaata tcaacagcaa cggattgtga actttattct
     2281 gccagatccc aatgcgacct tcggcgcgac atacaatgta cagcaggctc tcatcgccat
     2341 tgggtcgggt ggtctgtttg gcgagggcta taactcgggc acacaaaccc aattacgatt
     2401 tctcaaggtg cgccacaccg actttatttt ttctgcgatc tctgaagagt ttggtctggt
     2461 aggttcatta tttgttattt tggttatcgt gttagtgatc tggaggtgcc tgcgtaccgc
     2521 gcaaaaggca cgggatgttg ccgggatgaa tatcgcttac ggtgtggcga cgttgatttt
     2581 ctttcagggc atggtcaata tcggcgtaaa tctcaatatc gtccccgtct ctggcttgcc
     2641 gttgccgttc atcagttatg gcggaagcgg ccttacgtca ctgatgctgg gtattggatt
     2701 agtgcaaagt gttgctatgc ggcacaagca aatggatttt tgatgagccg atggttgagt
     2761 agctcggagc gctttttgcg aggagcatat cgaaaccatc atttgcaaat aaatatgaaa
     2821 ttgggagaat attttgacga tcaaaccagc acgtacacgt atggcacctt caccgactgg
     2881 tcgttttcat ctgggcggcg cgcgcacggc cctatttgac tacttgctcg cgcgccaaac
     2941 aggcggacag tttattttac gccttgaaga tacagaccaa aagcgattcg ttccgggctc
     3001 ggaggaggag atcctccgca gcttggattg gctgggaatc acaccagatg agagtccggc
     3061 acacggagga aagtatggcc catatcgcca gacggaacgg cgcgaaagtt atctgaagca
     3121 tgctcaaatc cttgtggaaa aggggcatgc ctatccctgc ttttgcagcc ccgaacgctt
     3181 ggaaaagatg cgcaaagaac aggaagtaca aaaggtggat cgtgtccagt acgacggctt
     3241 gtgccggcgg attgatccgg atgaggcagc gcggcgcgtt gccaatggtg aaagttatgt
     3301 aatacgtttc aaaatgccaa aagaaggaac cacgaccgcg catgatcact tacgcggcga
     3361 tattgtcaca gaaaataaat atctggatga ttatgtaatt ctgaaatcag atgggctgcc
     3421 cacgtatcac ctcgcggcga tagtggacga ccacgaaatg gagatcacgc atgtgttgcg
     3481 cggctctgaa tggctttcga cgtttccgct acacgtgcag atcgtacgtg cctttggttg
     3541 ggaggagcct gtttggatcc atctctcggt gtttctaaag cccagtggca agggcaagat
     3601 gagcaaacgc gaatctgctc aagccatgaa agacggttat tcgattttcg tcgacgatat
     3661 gaagggtctc ggatacatcc ccgaaggtgt actgaattgg attgccttga tggggtgggg
     3721 cgttgctgaa gatgaattaa tgacacttga tcaaatgatc caacgcttca gcattgacac
     3781 attgactcct tcaccgacgg cgatcaactt ccaaaagctg gaccatttca acggcgcaca
     3841 tattcgtctt ctctcaactg aagacctggc agcgcggatc aagccctatt tcaccgacgc
     3901 aggcttggag gtcagggatg acatgttgct caagatcacg ccattgatcc gcgagcgtct
     3961 cgtcacactg gatgattgcc ttgcgtttgc aagttttttc ttcaaaggag aggtgcagcc
     4021 aacccctgaa gacttaatcg caaagggact ggatgccacg ggatccgctg agatcgcgaa
     4081 aaaagtttat gagattctca acgcattgcc cgatgtatcc catcaaaccg cggagccgcc
     4141 gttgcgttcg tatgtcgaat cgtccgggct gagccccaat caggttttcg gcattttgcg
     4201 tgtggctgtc accgggcaaa aggttagccc accgctcttt gagagcatgg aggtgatcgg
     4261 gcgtgaaaaa gtgttgccgc gcttgaaaaa cgccattgac cttttggaga aggtatcgca
     4321 ttaaaaaatg aactccgcgc tttggttgcc gcggagttcc tgatggttat atctttctgt
     4381 tgtatcgatt gtatggggtc ttattatatc gagaccaacg ttcgtacttt cgccagaatc
     4441 gacgggcgat ttcgcccgtc atggtagcaa ttcgctcctc gtttttctcg ctgatcccca
     4501 ttcctttgcg tgtaagcacc cagtcgaaaa tcaggtgttg aacttcgtgg gcgactaact
     4561 cgtgcgccat gggagaaaaa ttgagctcgc tcaggtaaat atacccaaaa agcccctttc
     4621 tttgcttgcg tacttccgcc gcaatgtagt aggcgtggca ctggttcctg cgcttgaagc
     4681 gggtcgggtt atcggcaaca tacacgttga accactgacg attgtcatta aaatggatgc
     4741 ggaaggcggg tttattcacg gttaaggtcc ttttatttag tgtttttagt aggtttactg
     4801 attataaatc ggatgctcgc cctccgaaaa attttttaag atttaaaatc aaaattgcaa
     4861 ttttatttaa gggcgcgcaa aggggagggg gagtcaaacc cagattttcg catagtcaat
     4921 tcgagattcg gtctggaatt cggcttttcg tcggtttttt gttgaaaata tcaggcaggg
     4981 gattccctat taagcacagg gaaaaatcct tattgaccat agtttctgtc tcctcgataa
     5041 tagttagaga gttagatcta tatcaggtct ctctcttata aaccatagaa gaaagcttgt
     5101 catgctcagc ccatgcttgc agaaatttga acaggcgctg agtctgacat acgaaaggaa
     5161 aatgattatg aatagaaaaa caacattccc tatggctttg ctgcttttcg tgcttctttg
     5221 gggcgcgttt ggtttcgttg ctgggccatc acaagcgcag agcgtagcat ttcagaccac
     5281 tcctgtcgtg agtacaccgg ctgttgaaac caccctggtt gcgccggttg tgacacagct
     5341 agtccctgtg acaggtacca ctgaccccag cccgatgatg tggatcttct ttggtctgat
     5401 cgcgctattg ggtgtcgtct tcctggtggc tttgttggcc ggttccggtg ctcacagaca
     5461 cgacggccca ccgcctcctc cataacagct tgcaacagta aagaaaatat gcgaatctcc
     5521 cctcaggact gaggggagat tcttttaatc ataaagtaaa caggctattc aaatccaacc
     5581 cctgtgtggg accgactgag ataactgctt attccatcac attatagtca atcacatacg
     5641 cggggatgtt gacacccgaa atcgaaatac ttatctcgtg agccgggatc gcagcatcgg
     5701 tggggatcat gtccatggcc tcttttgtaa cgagaatttc gccggcggcg gctacatctt
     5761 ctcccatttt cgatgcgcgg ttgacggggt caccaaaaca atcttcatgg ccaatgatca
     5821 acagtttgcc gtaatcaatg ccgcaggaga tgtgaatatc aaggtcatcg gaggtaagca
     5881 gattcgaggc tttgaaggca tgttgcattg caaccgccga attgaccgcg gcaagcggtt
     5941 cggggaagac agcaaagcaa ttatccgctt catacttgag catccgccca ccataggatt
     6001 tgacaatcgg ttccgtagtc aactgcatac gccgtaccat cgaaaggtaa tgaacgattc
     6061 catatttacg cgtgagcatg gaaaaccccg acatatccag cacaaagacc gcgaactccg
     6121 ctccatagtc attccaaaga gtctcctcca cttttttgcg ttcactcaac tcttcggttt
     6181 gagagaagct aaggagaaga tcctgaaact tttttgcagg ttttaccggt cgtgatggca
     6241 tgttaaatgg gtccggaggt attatatgtc cgatgggtcc aacagctctt gtgtgctggg
     6301 gaggttcaga gtgtaagtgg aatgttcttc tttatttaat ggtaggcata aggtcaggtt
     6361 tccggtgtct aacagatctt aaatgtcaga cctgtgagac tacttcgact tgttagactt
     6421 ttctatcttc gcccagctat ccttgaggga aactgttaaa ttgaagaccg gcttctcggc
     6481 ggtggaatcc gggtcggtgg caaaatatcc ctggcgttcg aattggaaac ggtcaccagg
     6541 ctgcgtctgc gcgagaagcg gctccacata tccggtttga acagtcagtg agttgggatt
     6601 caaggcctcg atgaatcctt catcgccttc ctcgggattt tccttcgtaa agagtcgatc
     6661 atacatgcgg atttccgccg cttttgcgtg tgaagcggat acccaatgaa tcgtggactt
     6721 cacc
//
LOCUS       DASTJP010000576         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1406595, whole genome shotgun sequence.
ACCESSION   DASTJP010000576 DASTJP010000000
VERSION     DASTJP010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406595"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1966)
                     /locus_tag="VFQ23_16910"
     CDS             complement(11..1966)
                     /locus_tag="VFQ23_16910"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HET9908331.1"
                     /translation="MKTPLWIPSQEYRQNAAITRFINTINARQDLNLHSYAGLYAWSV
                     ENIPEFWAAVWDFAEIRHSQKYDRVVDDLTKFPGTKWFPSARLNFAENLLRYRDDQLA
                     FIFKGETQVTKRMTYAELYDSVARLAHSLRDAGVGVGDRVVGYMPNLIETATGMLAAT
                     SLGATWSSCATDIGPAAAIERLGQIEPKVLITADGYFYKGKSFDTLDHAAEVARGISS
                     LKKVIVVSYTREKPDISQIPNAVHYDEFLSKEKNLNIQFEQLPFDHPLYIMFSSGTTG
                     KPKCIVQGAGGVLINHMKELLLHTDLKRSDKIFYITSCSWMMWNWLLSSLAVGATIVL
                     YDGNPNYPDPGAMWKLIQDEKITIFGCSASYIHFLKKENVSPRKQYNLSSLREISQTG
                     SPLSADGFEYIYQEIKPDLHFNSISGGTDINGCFAAGSPILPVYAGELQSPALGMKIK
                     AYAEDGHPVYDEQGELVCEAPSPSMPLYFWNDPDGSKYHKAYFDVYPNVWRHGDYVVI
                     HGDTGGVTFYGRSDAVLKPSGVRIGTAEIYAQLENIPEISDSLAIGQNYQDDQRVILF
                     VKLVPGFALTDELKDKIRKTLRQNASPRHVPAVILETPDIPYTLNMKKVESAVTNIIN
                     GRTVSNKDALINPNSLEFYEQILSQLS"
     gene            complement(2027..2209)
                     /locus_tag="VFQ23_16915"
     CDS             complement(2027..2209)
                     /locus_tag="VFQ23_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908332.1"
                     /translation="MKSNNALVSLGLLFLIIAVTSSVTLWGDVSSPVKIAMFACGFSA
                     GIAIGTLIARRRNSAG"
     gene            complement(2218..2526)
                     /locus_tag="VFQ23_16920"
     CDS             complement(2218..2526)
                     /locus_tag="VFQ23_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET9908333.1"
                     /translation="MSADLRAVTSLDRLIHEPARLLVVTILASVVSADFLFLQRETGL
                     TKGNLSAHLSKLEEAGYVKIEKTFKGKLPLTICKLTSPGLKALKAYREQLQTFINDTS
                     "
     gene            complement(2528..3157)
                     /locus_tag="VFQ23_16925"
     CDS             complement(2528..3157)
                     /locus_tag="VFQ23_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908334.1"
                     /translation="MKENFSEVETRIKRYWYVDGFGELVGGGGMCLILAIYFAAGEYF
                     GDQSFIGGLLSSTMALVLIGGFFLVRRIINAAKERVTYPRTGYVEYPDAQRKIPSAIF
                     AAIGGALLAAIFVSMVRRFDSVDALVAISGIVMSLILSGKQLWSTRVRRFYLLSGAAL
                     IYGLALAISGLPRGYNLGLFYALMSLSFAVSGGLTLKKYLDENPLQPDL"
     gene            complement(3285..>3753)
                     /locus_tag="VFQ23_16930"
     CDS             complement(3285..>3753)
                     /locus_tag="VFQ23_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908335.1"
                     /translation="RIDDSSQAEISLRVGEPEILSSPSYQIDTEEVLIVTHRASPIQN
                     LTLDEARTLFAGQGDPSVQLWTYSSGEDVQDVFDQVVMDGRSVSSSAWIAVHPQQMSD
                     TLVNEPNTVGILPRRWKVGDSRLVYTVPNIPVLAVTPNEPEGAVRDLIACLQK"
BASE COUNT          971 a          977 c          876 g          929 t
ORIGIN      
        1 aaagtgactg tcaacttaat tgcgatagta tttgttcgta aaactctaaa gagttcggat
       61 taatcaatgc atccttgttg ctcacggtac gaccattaat aatattcgtc acggcgcttt
      121 ccactttctt catattcaac gtataaggaa tatcgggcgt ttcaagaatc accgcaggca
      181 catggcgcgg cgaggcattc tggcgcaaag tcttacgaat tttatctttc agttcatcag
      241 tcaatgcgaa gcctggcacg agtttaacga acaggatcac gcgctggtcg tcttgatagt
      301 tttgtcctat cgcaaggcta tcagaaattt cggggatatt ttccaactgc gcgtaaatct
      361 ccgctgtgcc tatacgcaca cctgaaggct tcaatactgc atcggagcgt ccatagaatg
      421 tgacgccgcc cgtatcaccg tggatcacca cataatcacc atgccgccag acgttcggat
      481 acacatcgaa gtaagctttg tgatatttcg agccatcggg gtcattccag aaatataacg
      541 gcatcgaagg cgatggtgct tcgcaaacca actccccctg ttcatcgtaa accggatgtc
      601 catcttcagc atatgctttg atcttcatcc ccagggccgg gctttgcaac tcgcccgcat
      661 agacaggcag gatcgggcta ccagctgcaa aacatccgtt tatatctgtg ccgccagaga
      721 tggaattgaa atgcaggtct ggtttgattt cctgataaat atattcaaag ccatccgcag
      781 aaagcggcga gcccgtctgc gagatctcac gcaatgagga aagattatat tgcttgcgcg
      841 gcgaaacatt ctcttttttc aaaaagtgaa tgtaactggc actgcatccg aagatggtaa
      901 ttttttcgtc ttgaatcaat ttccacatcg cacccgggtc gggataattg ggattgccat
      961 cgtacagcac gatcgttgca cccactgcca atgaactcaa tagccagttc cacatcatcc
     1021 aactacagga cgtgatgtaa aaaatcttat cactgcgttt tagatccgta tgcagcaaaa
     1081 gttccttcat gtgattgatc aacactccgc ccgcaccctg tactatgcac ttgggcttac
     1141 cggtcgtgcc ggaggaaaac ataatataca gtggatgatc aaagggcaat tgctcgaact
     1201 gaatgttcag atttttttct ttggatagaa actcatcgta atgaacggcg ttggggattt
     1261 gagaaatatc tggcttctca cgcgtgtacg aaacgacgat cactttcttc aacgatgaga
     1321 ttccgcgcgc cacctctgca gcgtgatcca acgtgtcaaa ggatttgccc ttgtaaaaat
     1381 atccatccgc ggtgataagt accttcggtt caatctgtcc caaacgctca attgccgcgg
     1441 ctgggccgat atctgtggcg caggaagacc acgtcgcgcc caggcttgtc gctgccagca
     1501 taccggttgc cgtctcgatg agattcggca tataaccgac gactcgatct cccacaccaa
     1561 cacctgcatc tcgcaaagaa tgagcaagcc gagcgactga atcataaagc tctgcatagg
     1621 tcatacgttt cgtaacttgc gtctcgcctt tgaaaatgaa cgcaagctgg tcgtcacgat
     1681 agcgaagaag gttttcggca aagttcaagc gcgcgctcgg gaaccatttc gtgccgggga
     1741 attttgtaag atcgtcaacg acccgatcat atttctgaga atgcctgatc tctgcaaaat
     1801 cccaaacggc cgcccagaat tcagggatgt tttccacgga ccaggcatac agcccggcat
     1861 acgagtgcag gttcagatcc tgccgtgcgt tgattgtatt gataaatcgc gtaatggcag
     1921 cgttttgcct gtattcctgg gatggtatcc acaagggagt cttcatgaga tattcctaat
     1981 attcagaata tctccattat acaattttac aaatccctcg gaatactcag ccagcggaat
     2041 ttctccgtcg cgcgattaag gtcccgattg cgatccctgc actaaatccg catgcgaaca
     2101 tggcgatctt gaccggggag gaaacatctc cccaaagcgt tacagatgag gtaactgcga
     2161 tgattagaaa cagtaatcct aaacttacaa gtgcattatt cgatttcata attctcctta
     2221 actggtgtca tttataaaag tttgtaactg ctcacgatac gctttgaggg cttttagacc
     2281 cggggacgtt agtttacaga tggtgagcgg taattttccc ttgaatgttt tttcaatctt
     2341 tacgtaaccc gcttcttcca acttggagag gtgtgccgag agatttcctt tggtgagccc
     2401 ggtttcgcgt tgcaggaaga ggaagtccgc gctaaccacc gacgccagga tcgttacgac
     2461 cagcagccgg gccggttcat ggatgagccg gtcgagggag gtgacggctc gtagatcagc
     2521 gctcatatca taggtccggt tggagcggat tttcgtccaa atatttcttc aaggtcagtc
     2581 caccggatac ggcgaaggaa aggctcatca aggcatagaa caaccccagg ttgtagccgc
     2641 gcggcagccc gctgatggcg agcgccaatc catagatcag ggcagcgcca cttagcaggt
     2701 agaaacgcct gacccgcgtg ctccagagtt gtttaccaga aaggatcaga ctcatcacaa
     2761 tccctgagat ggcaaccagc gcatccaccg aatcgaagcg ccgcaccatg gacacaaaga
     2821 tggccgccaa caacgcgcca ccgatcgctg caaagatcgc tgagggaatt ttgcgctggg
     2881 catccggata ttccacgtag ccggtacgcg gataggtgac gcgctccttc gccgcgttga
     2941 tgatgcgccg cacgaggaaa aaaccaccga tcaacaccaa ggccatagta gagctcagca
     3001 atccaccgat gaaggattga tcaccaaagt actcacccgc cgcaaagtag atggcgagga
     3061 tcaggcacat gccaccgcca cccaccaatt cgccaaaccc gtctacatac caatagcgtt
     3121 tgatgcgagt ctcaacttcc gaaaaatttt ctttcattct gtcctttttg atttttggtt
     3181 tgcactgcaa accagtttgc aactatatta ctcccatttg cataggctgt caagataaat
     3241 aaaaaaagcc cgactcacgc gagccgggct ggcaattttt gaagctattt ctgtagacag
     3301 gcgatcaaat cccggacggc accttccggt tcattcggcg tgactgccag cacaggaata
     3361 tttggtacag tatataccag gcgtgagtcc cccaccttcc aacgccgcgg caatatgccg
     3421 acggtgttgg gttcattgac taaggtgtca gacatctgtt gcgggtggac tgctatccac
     3481 gccgatgatg agacactcct tccatccatt acaacctgat caaacacatc ctgtacatcc
     3541 tcacctgaac tgtacgtcca tagctgcact gaaggatctc cctgccccgc gaacagggtg
     3601 cgtgcctcgt caagcgttaa gttttgaatc ggactagctc gatgtgtgac aatcaaaacc
     3661 tcttcggtat caatttgata cgaaggtgac gatagtatct ctggctctcc tacacgcagc
     3721 gagatttcag cctgagatga atcatcgatt cta
//
LOCUS       DASTJP010000577         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_372768, whole genome shotgun sequence.
ACCESSION   DASTJP010000577 DASTJP010000000
VERSION     DASTJP010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372768"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..>2728
                     /locus_tag="VFQ23_16935"
     CDS             322..>2728
                     /locus_tag="VFQ23_16935"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908336.1"
                     /translation="MSAPILATKLYAPPPRPRLVFRPHLIDQLNEGLRSSRKLTLISA
                     PAGFGKTTLVSEWITDCGRPTAWLSLDEGDNDPTQFLIYLVAALQTIVPNIGEGVLGV
                     MQSPQPPSIESILTTLLNEITTIPDNFVLVLDDYHLTDSKAVDNALTFLLKHLPPQMH
                     LVITTREDPQLPLARLRSHDQLTELRVADLRFTSSEAAEFINQIMGLNLSAEDIAALE
                     ARTEGWIAGLQLAAISMQGHQDTTNFIKSFTGTHHFVLDYLVEEVLYQQPENIQKFLL
                     QTSILDRLCGPLCDAVLLDPSASGQDTLEYIEQANLFIVPLDTERRWYRYHHLFADLL
                     RQRLGQSHTPEELAQYHICASEWFEYNGDIGEAFHHAVAVADFERAARLLESSWLSMD
                     ETFQTGTWLGWANQLPLNVRRLRPVLLTQIGWSYMNAGNVMLSESSLRDAENSLKRPR
                     EELVIVEAEQFRTLPARIIFARAYNAQTQNRFDDVVRYVETTLDMIPHEDQYMQAQAS
                     SILSTTYWASGELDKSFELMSNWVNAAQQAGNIVFAVAASFGKADILITQGRLRDAMQ
                     VYQTALSLAAEHGAEQHTAHHHLGLGLLHHEMGEDEPAAHHLQKAFELGRQTTIVDWM
                     YRKSLAQAYLKESEGDLRSALELLNDAQRFYVRTPIPNLRPVEAMQARIHIKQNHLNK
                     AQVWAHQSGLSLQDKPIFLNEFGYLTFARIMLAENQNDQHFQTMLQMLESLLKQAESQ
                     NRLRSRIDILITQALAFSAKDSAKALAALEQALTLAEPEGYLRLFVDEGKPMAELLSK
                     FKSS"
BASE COUNT          668 a          735 c          687 g          638 t
ORIGIN      
        1 ctccatcgcg gtttcgaatc ccgtcgggac cgccaagaat aacgcaaaag aaccgatcaa
       61 aaaggtcgtt tttttgttgt ttaatgccga ttttgcgtta aatttcgcag gtttttttag
      121 ttacgctcca cgggttcgta aaacggcgtt ttctaatcga acccgcgatt tagcataacg
      181 gttgcgagtt agggtagtat gtcaagagtt gtgtaaaagt tgaagggagg gtgcacacca
      241 tccaggaaat acccgggata gggggcgata ctacttattt cggaaaccgc caaattattg
      301 catgtacctt ataatgaatc catgtctgcg ccaattttag ccactaaact ttatgctcca
      361 ccgcctcgac cacgcctcgt cttccgccct cacctgatcg atcaactgaa cgagggtctc
      421 cgttcgagtc gcaaactgac cctcatctct gccccggccg gctttggtaa aaccacactg
      481 gtcagcgaat ggatcacgga ttgtggacgg ccaaccgcct ggctgtcact ggacgaaggg
      541 gataacgacc ccacacaatt tttgatatac ctcgtcgctg ctttgcagac gattgtgccg
      601 aatattgggg aaggggtatt gggtgtgatg caatcccctc agccaccgtc aatcgaatcg
      661 attctgacga ccctgctcaa tgaaatcaca accatcccgg acaatttcgt ccttgttctt
      721 gacgactacc accttaccga ttccaaagcg gtagataatg ccctcacctt tctgctcaaa
      781 catctgccac cacagatgca cctggtcatc accactcgcg aggacccaca actgcccctg
      841 gctcgattac gttctcacga ccaattgacc gaattgcgcg ttgcggattt gcgtttcact
      901 tcctccgaag ccgctgaatt tatcaatcaa ataatgggac taaacctttc agcggaggac
      961 atcgccgcgc tggaagcccg caccgaagga tggattgctg gtctgcaact tgctgcaatt
     1021 tcaatgcagg gacatcaaga caccaccaac ttcatcaaat cgtttacggg tacacatcat
     1081 tttgtgctgg attatctggt tgaagaagtc ttataccagc agcccgaaaa catccagaag
     1141 ttcctgttgc agacatccat actcgaccgc ctttgcggcc ccctttgtga cgccgtcttg
     1201 ctcgacccct ctgcttccgg gcaggacacc ctggagtata tcgaacaagc gaatctgttc
     1261 atcgttccct tggacaccga gcggcgttgg tatcgctatc accatctatt tgctgatttg
     1321 ctgcgacagc ggctgggaca aagccataca cccgaagagc tagcccaata ccatatctgc
     1381 gccagtgagt ggtttgagta caacggtgat attggagagg cattccatca cgccgtcgcc
     1441 gttgcggatt ttgaacgagc tgccagattg ttggagagta gttggctgag catggacgaa
     1501 acctttcaga cgggcacctg gcttgggtgg gcaaaccaac ttccgttgaa tgtgaggcgt
     1561 ttgcgtcccg ttctgttgac tcagatcggc tggtcataca tgaatgcggg caatgtcatg
     1621 ctgagcgagt ccagtttgcg ggatgccgaa aattcattga agcgccctcg cgaagaattg
     1681 gtgatcgtgg aagccgagca attccgcacc ctgcccgcac ggatcatatt cgcccgtgct
     1741 tacaatgcgc aaacgcagaa tcgttttgac gatgtggtca gatacgttga gacaacgctg
     1801 gatatgatcc cgcatgaaga ccagtacatg caggcgcagg catcgtcgat cttaagcacc
     1861 acgtattggg cgagcggcga gttggataag tctttcgagt taatgagcaa ctgggtgaat
     1921 gcagctcagc aagcgggcaa tattgtgttt gcggtggcgg catcattcgg caaagcggat
     1981 attctcatca cgcagggtcg cttgcgcgat gccatgcagg tctatcaaac ggcgttgagt
     2041 ttggcggcag aacacggcgc agaacagcac acagcgcacc atcatttggg gctggggctg
     2101 ctgcatcacg aaatgggcga agatgaaccc gccgcgcatc atttgcaaaa agcatttgaa
     2161 cttggcaggc aaaccaccat cgtggattgg atgtatcgca agagtctggc gcaggcgtac
     2221 ctcaaagaat cagaaggcga cctgcgcagt gcgctggaat tgctgaacga cgcacagcgt
     2281 ttttacgtcc gcacgccgat ccccaacctg cgcccggtgg aagccatgca ggctcgtatt
     2341 cacatcaagc aaaaccactt gaacaaagca caggtatggg cacaccagag cgggctttca
     2401 ttgcaagata aacccatctt cctgaacgag ttcgggtatc ttaccttcgc ccggatcatg
     2461 ctcgctgaaa atcagaacga ccaacatttt cagaccatgc tgcaaatgtt ggagtctctc
     2521 ttgaaacagg cagagagtca aaaccgcctg cgcagccgca tcgatattct catcacgcaa
     2581 gcgctggcgt tttctgcaaa ggattccgcc aaagccctcg ctgcccttga acaagcgctg
     2641 accctggcag aacccgaagg ttatttgcgc ctctttgtcg atgagggcaa acccatggca
     2701 gagttgttgt ccaagttcaa gagcagta
//
LOCUS       DASTJP010000578         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_889081, whole genome shotgun sequence.
ACCESSION   DASTJP010000578 DASTJP010000000
VERSION     DASTJP010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889081"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFQ23_16940"
     CDS             complement(<1..564)
                     /locus_tag="VFQ23_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9908337.1"
                     /translation="MFEILFLGTSASAPSAKRGLSAQIIKHDEYRFLIDCGEGTQRQI
                     LQSGTGFKNLTRILLTHGHLDHILGLGGLMSTFLRWEAVEELDIFGGRSTLDRVHDLL
                     FGVVLRGNTPPMPLRMNEIRPGMFFEADDFTLSAFSVTHRGPDCLGYVFEGKARRPFL
                     PAKADEQLRIIAKKFCGRGSDLLRCLRP"
     gene            809..3151
                     /locus_tag="VFQ23_16945"
     CDS             809..3151
                     /locus_tag="VFQ23_16945"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET9908338.1"
                     /translation="MPLKTPSPASKSNSGPAGKPKGKTVTPGRGKSESKAAPKKKTQP
                     AAPPQPIISLSWWDTLTPERKLDVVGAIMSVVGLLILLVLFSAQRSALTGNMIRVLGQ
                     ILGWGIYILPIALIGMGTWLILRRIEKLPPLSLERATGIILFFLWLLTTMHSIIASPD
                     MAGQAAIDGAGGGYIGSLFERALFNSFGIWGAIVALLAWLLITLTMIFDISVQDLFRW
                     FNPLFVKIREILNTPIRQTGEPIAPEDAAVASNGYTPLHRAEPAVKMQAPLTPVTTIK
                     TAEAVINWQLPEAKNILDLGSAPSLNDEFIEQRARLIEETLASFGAPVQVVVTNRGPT
                     ITQFGVEPLFVETRTGRTKVRVNKIASLADDLALALAAPRIRIQAPVPGHSYVGIEVP
                     NDEMTLVALRDIVESEPFQRNKHALRFGLGRDVTGNPIMATLENMPHMLIAGTTGSGK
                     SVCVNAILTCFLLNNTPDDLRLILIDPKRVELTGYNGIPHLLSPVVVEIDRVIGALQW
                     MTREMDKRYHMFAQVGARNITDYNARMKLQGTKKLPFLVVVIDELADLMMIAPGETEQ
                     TITRLAQLARATGIHMILATQRPSVDVVTGLIKANFPARIAFAVASNTDSRVILDQPG
                     AERLLGRGDMLFQAPDAPSAVRLQGVFVSDHEIQNLVEFWRAQLGGTSPYAASSSSVP
                     GGMPEPGVPLKQTAMFDEMNLEEANTDPLLPEAIDLVRREGRASVSMLQRRMRIGYTR
                     AARIVDMMEDKGIVGPPEGTTQVRSILDYGPTAPPKDDGA"
BASE COUNT          772 a          849 c          834 g          732 t
ORIGIN      
        1 ggggcggaga cagcgcaaaa gatcggaacc cctgccacaa aacttttttg caataattcg
       61 taactgttca tccgctttgg ccggtagaaa tggacgtcgc gcttttcctt caaaaacata
      121 ccccaggcaa tctggcccgc ggtgagtcac agaaaaagcg ctgagtgtaa aatcgtccgc
      181 ctcaaagaac atgcccggcc tgatttcatt catccgaagt ggcatgggag gtgtgttgcc
      241 gcgtaacaca acgccaaaca acaaatcatg aacacgatcc aacgtggagc gcccgccaaa
      301 aatatccagc tcttccacag cctcccaacg cagaaaggtg gacatcaatc ccccgagccc
      361 caaaatatga tcgaggtgcc catgcgtcag caaaatgcgt gtgaggtttt taaagccggt
      421 ccctgattgc aaaatttgcc gctgcgtacc ttcaccgcag tcaatcaaaa aacgatattc
      481 atcgtgctta ataatttgcg cagaaagtcc gcgtttcgcg gagggcgcag aggcagacgt
      541 tccaagaaat aatatttcga acaaaatgga atccttaggc agacaaaatt tgccacagag
      601 catacagaag attcctagat gtttctcatc gttctctgcg atctctgtgg cgaatgatct
      661 attggttttt ttattttacc ctacgaagta ccgcatcatg tagtcatact aaaaattatt
      721 ttttatgcac aacttgcctg gaaaaactcc acaacccata agaatgaggg taaaagaaaa
      781 acagaccatt tgtgctatac tttaactcat gcctcttaag actccatccc cggcatccaa
      841 atcgaactcg ggtcccgctg gaaagcccaa aggcaagacg gtgactcccg gacgcgggaa
      901 gagtgaaagc aaagccgcgc caaaaaagaa gacacagcct gctgcgccgc ctcagcccat
      961 tattagcctt tcgtggtggg atacactcac acccgaacga aagctggatg ttgtgggtgc
     1021 gatcatgtct gtagtaggtc tactcattct tttggttctg ttctccgcac agcgcagcgc
     1081 cttgaccgga aacatgatcc gtgtgctggg gcagatcctg ggttggggga tttacatttt
     1141 gccgatcgcg ttgatcggga tgggcacctg gctcatacta cgccgcattg aaaaacttcc
     1201 gcctctttca ctggaacgcg ccactgggat cattttgttt ttcctgtggc tactgacaac
     1261 catgcattcg attatcgcct cgccagatat ggccgggcag gctgctattg atggggcagg
     1321 tggtgggtat attggtagtc tgtttgagag agccttattc aacagctttg gtatctgggg
     1381 ggcaattgtg gctttgctcg cctggttgct tatcacgctg acaatgatct tcgacatctc
     1441 tgttcaagat ttgtttcgct ggttcaatcc gcttttcgta aaaattcgcg aaatccttaa
     1501 tactcccatt cggcaaacgg gagaaccgat tgcacctgaa gacgccgcag tcgcatcgaa
     1561 tggatacacg ccattgcatc gagctgaacc tgcggtgaag atgcaggcac cgcttacgcc
     1621 agtgacaacg atcaaaacgg cggaggcggt catcaattgg cagttacccg aagcaaaaaa
     1681 tatcctcgac ctaggctctg cgccctcgct caatgatgaa ttcattgaac aacgtgcgag
     1741 attaattgaa gagacgttag cgtcatttgg ggcgccggtc caggtagtcg taaccaatcg
     1801 cggaccaaca atcacgcagt ttggtgtgga gccgctcttc gtcgagacga gaaccgggcg
     1861 cacgaaggtg cgcgtcaaca aaattgcgtc gctggcagat gacctggcgc ttgcgctcgc
     1921 cgcgccgcgc atccgcattc aagcgccagt accgggtcat agttacgtgg gcatcgaggt
     1981 tcccaacgac gaaatgactc tggtggcctt gcgcgacatc gtcgaaagtg agccatttca
     2041 gcgcaataaa catgctttgc gctttgggct cgggcgcgat gtcaccggta atccgattat
     2101 ggctaccctc gagaatatgc cacacatgct gatcgcaggg acgacaggtt ctggtaaatc
     2161 cgtttgtgtc aatgcgattc tgacctgctt cctgctgaac aacacgcccg atgacctgcg
     2221 cctgatcctg atcgacccca agcgcgtgga gctgaccggc tataacggta tccctcactt
     2281 gctgtccccg gtggtagttg agattgaccg cgtgatcggc gcgctgcaat ggatgacgcg
     2341 tgaaatggac aagcgttatc acatgtttgc acaggtcggt gcgcgcaaca tcaccgatta
     2401 caacgcgcgc atgaaattac agggaacgaa gaaactgcct ttccttgtgg tggtgattga
     2461 cgaactggca gacctgatga tgatcgctcc gggcgaaacc gaacagacca ttacacgtct
     2521 cgcgcagctt gcgcgtgcca ccggaattca tatgatcctg gcaacacagc gcccgtcggt
     2581 tgatgtggtg acaggcttga tcaaagcgaa cttccccgcg cgtattgctt ttgcagtggc
     2641 gtccaacacc gatagccggg tgatcctcga tcagccgggt gcagagcgcc tgctcggtcg
     2701 cggtgatatg ttattccagg cacccgatgc accttccgct gtgcgtttgc agggtgtctt
     2761 tgtctcagac catgaaattc aaaatctcgt ggagttctgg cgcgcgcaac ttggcgggac
     2821 cagtccctac gccgcatctt cttcctctgt tcccggaggt atgcccgagc ctggcgtacc
     2881 actcaaacaa acagcaatgt tcgacgaaat gaatctggaa gaggcaaata cagatccctt
     2941 gctgccagaa gccattgacc tggtccgccg tgagggacgc gcttcggttt ccatgctgca
     3001 gcgccgtatg aggatcggtt atacgcgtgc ggcgcgcatc gtggatatga tggaagataa
     3061 agggattgtc ggtccgccag aggggacaac ccaggtacga tccatactgg attatggtcc
     3121 cactgcgcca ccgaaggatg atggggcgta atgcatgcgc aaccacgccg taggcgggtc
     3181 gctcctg
//
LOCUS       DASTJP010000579         9956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_524935, whole genome shotgun sequence.
ACCESSION   DASTJP010000579 DASTJP010000000
VERSION     DASTJP010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524935"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFQ23_16950"
     CDS             complement(<1..586)
                     /locus_tag="VFQ23_16950"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9908339.1"
                     /translation="MMSHKFRSQQKWILRLLRTSLTILILSLLISPGATSAAPSNQNE
                     LLTVRFSTFNASLNRNFAGQLVSDLSTPNNVQAKTVAEIIQRVRPDVLLINEFDFVEN
                     GVAAELFQDNYLSVSQNGAAPIEYGYYFVAPSNTGIPSGFDLNNNGVIAGGDDAFGFG
                     FFPGQFGMAVYSRYPINYDDIRTFQLFLWKDMPGA"
     gene            complement(854..2557)
                     /locus_tag="VFQ23_16955"
     CDS             complement(854..2557)
                     /locus_tag="VFQ23_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HET9908340.1"
                     /translation="MQRSVRLFSLIILLAMLLTSAVSAGATPSGTVNVQILALNDFHG
                     NLEPPTSSSGCVPAVSGCPAPNVPAGGVEYLATHINNLRALNANTVVVSAGDLIGASP
                     LLSALFHDEPTIEAFNQIGLDFNAVGNHEFDEGWRELKRMQEGGCHPVDGCQDGDEFL
                     GASFQFLAANVVRKDNGKTIFPAYKVRSFAGAKVAFIGMTLEGTPLIVTPSGISDLNF
                     LDEAATVNALVPELKAKGIETIVVLIHEGANQNPITSPFNSCVGVSGPIVDIVNSFDD
                     EVDVVISGHTHQPYNCVIDGKIVTSAFSFGRLVTKVDLTIDRGTGEVVTMVAENKIVT
                     RDVPKDSLITSLIAKYNAFAAPIANRIIGSITGPITRTANLAGESALGDVIADGQLAA
                     TDDPGFGEAVVAFMNPGGIRADLTFPSSPANEGDGNVTYGEMFTVQPFGNSMVTMTLT
                     GAQIERLLEEQFAGCTFGSNRILQVSAGFSYTWVASGPVCDKVDPSTIMLNGQVVVAT
                     NTYRVTVNSFLADGGDSFPVLTQGTARLGGAVDTDAFEAYFATHSPVAPGPQNRITRV
                     P"
     gene            complement(2911..4689)
                     /locus_tag="VFQ23_16960"
     CDS             complement(2911..4689)
                     /locus_tag="VFQ23_16960"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HET9908341.1"
                     /translation="MKKSVRLFSIFMMLAIALSGTVSAGALKPDPPAVLVPLQLLAFN
                     DYHGFLEAGGNPGPGTIGTAAAGGGEFLSTKLKELRAGKENTLTVAAGDLIGGSPFLS
                     GLFHDEPSVESLNEMGLDVSSVGNHEFDEGVTELLRMQNGGCHPEDGCYFPDAPYPGA
                     DFQWLAANVVNDATGETALPPYWIQKFGGVKVAFIGMTLEDTDTLVAPSGVAGWSFLD
                     EAETANALVPVLHDQGVQAIIVLLHEGGSQTPPPGAVNACVGISGPIVAINSLLDPAI
                     DVVITGHTHLPYNCMLPDPAGNPRFVTSAFSAGRVVSEFNLVLDKRTRDVRRDLSTAV
                     NHPVFQAQVARDPAITAIINKWNALAAPLGSQVVGTITGDIRRAFNGSAEDRASESNA
                     GNMIADAQLWATQENGAQIAFMNPGGIRSDFIFAKSGAELVDGIVTYAEAYNVQPFSN
                     ILQTIPMTGAQIDAVLRQQCQPIGVARPFLHLGVSAGFTYDLSKTFALVDHDNNPNTA
                     PVNSCTSITVANIRLNGEPLNPTATYIVTVNNFLADGGDNFTVFRQVDPALRVGGGID
                     LDELVNYFGSASPISPPGTNRVTELP"
     gene            4960..6336
                     /locus_tag="VFQ23_16965"
     CDS             4960..6336
                     /locus_tag="VFQ23_16965"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908342.1"
                     /translation="MRSLEYIIPILLVVYLLFPGPRPVFLRILPAGALILTIIHIAID
                     GYRWQMIPIYFLTLLLVIISLNRFWDIQPPVSYVTVLVLAVSTALPFLLPVPSIPIPS
                     GPYPVGTRIYELVDSSRKEIYSGKDEARRFMVQIWYPSEASSSDERAPWMENADIFSP
                     AIATYIDLPSFFLDHLALVKMPAYRESKIAPSSTPYPIVLFSHGWNGFNAQNSGQAIQ
                     VASHGYVVVGIQHPYGALITVFPDGAIVNNNPSALPDGQPTEEYESAAHILSNQWAGD
                     MGFSLDFLESQNDDASSPFHNLLDLSNVGVYGHSTGGGASIEFCGNDDRCKAVLGMDP
                     FMRPVSYEVIDDGLTQPAFFMFSQRWADDVDSRNNELFHRFIPHVSQSVGVIIIDGTA
                     HYDFTDLPLLSPLAPQLGLKGPISGKHVTTIVNDYLLSFFDMTLKGTSTNLFDGKNPK
                     YTEVRVKQ"
     gene            6378..6776
                     /locus_tag="VFQ23_16970"
     CDS             6378..6776
                     /locus_tag="VFQ23_16970"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9908343.1"
                     /translation="MERIPAIELDLVQEFVAKAHGDFQRVAELLEQEPGLINATWDWG
                     GGDFETALGAAAHMGHKEIANYLLERGARLDLFAAAMLGKLDIVKAILNAYPNAINFP
                     GPHDISLIAHAQAGGEDARAVLAFLEGLQS"
     gene            complement(6953..7927)
                     /locus_tag="VFQ23_16975"
     CDS             complement(6953..7927)
                     /locus_tag="VFQ23_16975"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908344.1"
                     /translation="MMRICVLSDEEITDFNPTPFMQGYDWEMVTMTDPVREVLRSLDA
                     RKEFDIYLNLCEGYENDGEVDDQAYQGIDVVRALEEMNLPFTGANSKFFDPTREEMQA
                     AAEAKGVSFVRGYRVTSVEEAERLVKNLRFPIMVKHPKSYGSTGMFRESRVDTPEQLA
                     PQIERVCSEFGAARMEEFIVGKEFNVLVVDNPDDFNNPIAYPPAELVFPPGEEFWHTD
                     VKWNYDVPFDFKRVTDPDLIERLHTLARGMYKAMNGNGYGRCDIRMNNQGELFILEIN
                     PNGGIMFKPEEYGPADYMILYDPGGYKVFFDRMFEVAIARQQMRAAAN"
     gene            complement(7933..8892)
                     /locus_tag="VFQ23_16980"
     CDS             complement(7933..8892)
                     /locus_tag="VFQ23_16980"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908345.1"
                     /translation="MQLCVLSDSEIKEFDPSQYLDEYVWEIFIPQRPVTEFVRALEKN
                     GNYDVYFNLCDGADNPQDDYDGIDIVRALENLQLPFTGANSHFYDPSREEMQAVAESN
                     GICFARGVNITNVDELQAVESLMYPLMVKHPQSFASTAMTRNSRVEAVSKLKEQVRRI
                     CNRFGSARVEEFIDGPEFTVLVVDNPEDLKQPFVYPPAEIIFPAGENFLHSHVKWKEW
                     VYLKPVNDIALSQSLMEMSRRMYLAMGGVGYARCDIRMGPDGKLYLIEINPNCGILFK
                     PEDLGPADVMMEYDPDGHAGFLDRIFRAAILRNQAAIKTANTK"
     gene            9011..9181
                     /locus_tag="VFQ23_16985"
     CDS             9011..9181
                     /locus_tag="VFQ23_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908346.1"
                     /translation="MSEQKPSRRASPIFLVLGIAFLAIGLATDQTVFTWLSVVFLVIS
                     LVRGGRWLRPRK"
     gene            9341..>9956
                     /locus_tag="VFQ23_16990"
     CDS             9341..>9956
                     /locus_tag="VFQ23_16990"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET9908347.1"
                     /translation="MSAKITRRDFLKAGGLTVLGAVGANALTNTTSDHGKHPTKVLAA
                     PAMQHDGHDGIMPTTVGEVDHEKNGFNPTDMLTDFDYGEVSTLPNGQVLREYNITALN
                     KNIEVVPGIEYPAWTYNGRIPGPTLRATEGDRIRINFSNGSNHPHTMHFHGFHPGNMD
                     GVPGSGPGGNVLPGGSFVYEFDAEPFGLHLYHCHAFPLARHIAKG"
BASE COUNT         2579 a         2538 c         2385 g         2454 t
ORIGIN      
        1 gcgctccggg catgtctttc cacaggaaaa gctggaaggt gcggatatca tcatagttaa
       61 tgggataacg cgaatagact gccatgccga attgacccgg gaaaaacccg aaaccaaagg
      121 catcatcccc accggcaata acaccgttgt tattaaggtc aaatcccgat ggaattcccg
      181 tgttggatgg tgcgacaaag taatacccat actcaatcgg agcggcaccg ttttgtgaga
      241 ccgacaaata gttgtcctgg aagagctcag cggcgacgcc gttttcgacg aagtcaaatt
      301 cgttgatgag taaaacatcg ggacgtactc tttggatgat ctctgccact gtctttgcct
      361 gaacgttatt gggtgtagag agatccgaaa cgagttgacc tgcgaagttg cgattgaggg
      421 aggcgttgaa cgtggaaaag cggaccgtga gaagttcatt ctgattcgat ggagcggccg
      481 aggttgcccc cggcgagatc agcagactta ggatcaaaat cgtcaaagac gtacgaagca
      541 agcgcaagat ccatttttgc tgtgagcgaa atttatgact catcattatt ctccttgaac
      601 ttggataaca tagaatcaga ctgcagcagg ggccaatgca cagtcatatc tcctcttcga
      661 taggattcaa actatttttg cccgatacac cacctttcaa caataaaatg tagagtgcaa
      721 tggtaggaaa ttgatcgact tttcgatgat tgtaatcaag cttggaagga tggtcaatca
      781 accataaagc gtaagaaaaa gaggaacgca cgacagtgac tgtcgtgcgt ttttttgata
      841 aacagtgttt cgattatgga actcgcgtga tgcgattctg tggtccggga gctacgggtg
      901 aatgtgttgc aaaataggcc tcgaacgcgt cggtatcaac tgcaccgccg agacgagctg
      961 ttccctgcgt gagcaccggg aaactatcgc caccatcggc taagaaggag ttcaccgtta
     1021 cacgatatgt attagtggca acaacaacct ggccattgag catgatcgtg gaagggtcta
     1081 ctttgtcaca aacagggcct gaggcaaccc aagtatacga gaaaccggcc gagacctgca
     1141 aaatacgatt cgacccaaag gtacagccgg caaactgctc ttccaataac ctttcgattt
     1201 gcgcgccggt tagggtcata gttaccatac tattgccaaa gggctgtaca gtaaacattt
     1261 caccgtaggt tacgttaccg tcaccttcat tggcagggct gctggggaag gtcaagtcgg
     1321 cacgaatacc accgggattc ataaacgcca ccaccgcctc accaaaaccc ggatcgtcgg
     1381 tagccgcaag ttgaccgtct gcaatgacat cgcccaacgc agattcgcca gctaaatttg
     1441 cggtgcgcgt aatagggccg gtgatggacc cgataatacg gtttgcgatg ggcgccgcaa
     1501 aggcgttata tttggcgatc aaagaggtga tcaagctgtc cttgggaaca tcacgcgtaa
     1561 cgatcttatt ctctgcgacc attgttacta cttcaccggt gcctcggtca attgtcaaat
     1621 caactttcgt cacgagacgg ccaaatgaaa atgcgctggt cacaatcttt ccgtcaatta
     1681 cgcagttata tggctggtgg gtatgaccac tgattactac atccacctca tcgtcaaagc
     1741 tattaacaat atccacaatc gggcctgaaa cacccacaca tgagttaaat ggcgatgtga
     1801 tgggattctg gttagcgcct tcatggatca ggactacaat cgtctcgatt cccttggctt
     1861 tgagttccgg cacaagcgca tttactgtag ctgcttcatc aaggaagttc aggtccgaaa
     1921 taccagatgg agtgacaatc aaaggtgttc cttcaagggt cattccaatg aaggccactt
     1981 tggcacccgc aaatgaacgg accttatagg ctgggaagat ggttttgcca ttatccttcc
     2041 gaacgacgtt tgcggcgagg aactggaagc tcgcgccaag aaattcatca ccatcctgac
     2101 agccatccac gggatgacag ccgccctcct gcatgcgctt caattcgcgc cagccttcgt
     2161 cgaactcgtg gttaccgact gcattgaagt ccaaaccgat ttggttgaag gcctcgatgg
     2221 taggctcgtc gtggaacaga gctgaaagaa gtggactggc accaatcagg tcgcccgccg
     2281 aaacgacgac ggtatttgca ttgagcgctc gaaggttgtt gatatgagtg gctaagtact
     2341 caacaccgcc tgctggaacg tttggcgcag gacagcctga gactgcggga acgcatccgg
     2401 aactgctggt cggcggctcg aggtttccgt gaaaatcgtt tagtgccaga atttggacat
     2461 tcaccgtgcc cgacggtgta gcaccagcgg agacagctga agtcaacagc atagccaaga
     2521 gtatgatcaa tgaaaacaaa cgaacagatc tttgcattag aaactcctcg tgatattgaa
     2581 ttggatttga gtgtatgtcg cagtgttgcg gtaatcgttg ccaggcacct cctcaaaatt
     2641 taaatcgcta cgctctatat tgaatgatta taattgccga aatactgcaa agggccttcc
     2701 ttatctaata aaaggttaca cgttgcatgc tgcgctagag agccgctggt tcctcccgta
     2761 atttcagaca agttatcgcc ataatctatg gcggagaaag gtatctggac gcgattagaa
     2821 ttttttaaaa acaacagccg agaaaccctc ggctgttgtt cattacattt tactgtcagc
     2881 agttccctga caagtaaatt attacagcag ttatggtaac tcggtcactc ggttcgtgcc
     2941 gggcgggctg attgggcttg ccgagccaaa ataattgacc agctcgtcca gatcgatgcc
     3001 accgccaacg cgtagcgccg ggtcaacctg acggaagaca gtaaagttat cgccgccatc
     3061 cgcgaggaag ttgttgaccg tcacgatgta tgtggcagtc gggttgaggg gctctccatt
     3121 taaccgaatg tttgcaactg tgatcgaggt gcaggagttg actggagcag tattgggatt
     3181 attgtcatga tccactagcg cgaacgtctt ggagagatcg taggtaaaac ctgcagagac
     3241 gccaagatgc aggaagggtc gagcgacacc aattggctga cactgctggc ggagcaccgc
     3301 atcgatttga gctccagtca tggggatcgt ctgcaggatg ttgctgaacg gttgaacgtt
     3361 gtatgcctcc gcataggtga cgatgccatc tacgagttcg gcgcctgatt tggcgaaaat
     3421 gaaatcagat cggatacctc ccggattcat aaacgcgatt tgcgcaccgt tctcttgcgt
     3481 cgcccacagt tgtgcatccg cgatcatgtt gccggcattg gattccgaag ctcgatcttc
     3541 agccgagcca ttgaacgcac gccggatatc acctgtgatg gtgccaacta cctggctacc
     3601 gagcggtgca gcgagcgcat tccatttgtt gatgatcgcc gtaattgcgg gatcgcgagc
     3661 gacctgcgcc tggaagacgg gatgattgac cgctgtgctg agatcgcggc gcacatctct
     3721 ggtgcgctta tcgagcacaa gattaaactc agaaacgacg cggccggccg agaatgcgct
     3781 ggttacaaag cgggggtttc cggctggatc aggcagcata caattgtaag gtaaatgagt
     3841 gtgacccgtg atcaccacgt cgatagcggg atcaagaagg ctgttaatcg caacaatcgg
     3901 gccactgata ccgacgcagg cattgaccgc acctggagga ggcgtttggg atccgccctc
     3961 gtgcaaaagc acgataatcg cctggacgcc ctggtcatgc aatacaggca caagagcgtt
     4021 agcggtctcg gcttcatcaa ggaaactcca gcccgcaaca ccagacggag cgacgagcgt
     4081 atcggtgtct tcgagggtca tgccgatgaa ggccaccttc acaccaccga atttctgaat
     4141 ccaatagggc ggcagggcag tctctcccgt tgcatcattg accacatttg cagccagcca
     4201 ttggaagtcg gcccccgggt atggagcatc agggaagtaa caaccgtcct caggatgaca
     4261 accaccgttc tgcatgcgga gcagttcggt gacgccttca tcgaactcgt gattgcccac
     4321 gcttgaaaca tccagcccca tctcatttag cgactcaacg gaaggctcat cgtggaacaa
     4381 tccggaaaga aatggtgaac caccgatcag atcgccagca gcgacagtaa gagtgttttc
     4441 cttgcccgct cgcagttcct tcaatttggt ggaaagaaat tcaccaccgc cagcagcagc
     4501 agtaccaatg gttccaggac ctggatttcc gccagcttcg agaaatccat ggtaatcgtt
     4561 gaatgccagc aactgaagcg gcaccagaac ggctggcggg tcgggtttta gtgcacccgc
     4621 tgaaactgtg ccagaaagcg caattgccag catcataaag attgaaaaca aacgaaccga
     4681 tttcttcatt taaagctcct cttaggaatg ttttacgttc gagtttaagc tataaagttc
     4741 ccgtaatctt gggccgggca ccacctttca tcgaaaatga ttgttccaat tgagattttc
     4801 acacaagata tacaaaagaa ttttagtggg ccgataacca aaacggctca ctaaaattgt
     4861 acggagaccg ttagcaaagg cgattttacc caccttaaag tcgagagtca acaaatcaag
     4921 tatattaaaa tgttacacat cctgttgtaa aggaatttta tgcgcagtct cgaatatatc
     4981 attcccattc tcctagttgt ttatttgctt tttccagggc cccggcccgt gtttcttcgg
     5041 atattgcctg ctggcgcgct gattctaaca atcatccaca ttgccatcga tggttatcgc
     5101 tggcagatga tcccaatcta tttcttaact ctcttacttg taatcattag cctaaatcgt
     5161 ttctgggata tccaaccacc tgtctcctac gtgacggttc tcgtactcgc tgtttcgaca
     5221 gcacttccgt tccttttgcc tgttccgtca attccgatcc caagcggacc ctacccggtg
     5281 ggaacacgca tctacgagct agtagactcc tcgcgcaagg aaatttattc aggcaaggat
     5341 gaagcacgtc ggttcatggt tcagatctgg tatccgtcgg aagcaagttc atctgacgaa
     5401 cgcgcgccat ggatggagaa tgcagacatt ttctcccctg ccatcgccac atatattgac
     5461 ctcccttcgt tctttcttga ccatcttgct ttggtaaaaa tgccggctta tcgagaatca
     5521 aagatcgcgc catccagcac cccctacccc attgttctat tctcgcacgg ctggaacgga
     5581 ttcaacgcgc agaactcggg gcaggccatt caggtggcca gccacggata tgttgtggtt
     5641 ggaatccaac atccttacgg tgcattgata actgtctttc cggatggagc catagtaaat
     5701 aacaatccat cggccttgcc tgatggtcaa ccaacggaag aatatgagtc cgctgcacac
     5761 attctgagca atcagtgggc cggcgatatg ggattttctc tggacttttt ggaatcgcaa
     5821 aatgatgacg catccagccc ctttcataat ctacttgatc tttcgaacgt tggtgtctac
     5881 gggcattcaa cgggtggcgg cgcgtcaata gagttctgtg gcaacgatga tcgctgcaaa
     5941 gccgtgcttg gtatggaccc ctttatgcgc ccagtctcct atgaggtcat cgatgatgga
     6001 cttacccagc ctgcgttttt catgttcagt cagcgttggg cagatgatgt ggacagtcgt
     6061 aataacgaac tgttccatcg gtttattcca catgtctcac aatctgtggg tgtcatcatt
     6121 atcgacggaa ccgcgcacta cgatttcacc gatctcccat tgctttctcc cctcgctccg
     6181 cagcttggtc tgaagggacc gatcagcggc aaacacgtga ccacaatcgt caacgattat
     6241 ctgttatcat tcttcgacat gacactcaag ggcacatcga cgaatttgtt cgatggaaaa
     6301 aacccgaagt atacagaagt tagggtcaaa cagtagtatt acttcattgc ccaccagcga
     6361 attagaagga gaacacaatg gaaagaatac cagccatcga gttggacctt gttcaggaat
     6421 tcgtcgccaa ggcacatggt gattttcagc gcgtcgcgga attgcttgaa caggaaccgg
     6481 gattgatcaa cgcgacctgg gattggggtg gcggagattt tgaaaccgcg ctgggcgccg
     6541 cggcacatat ggggcataaa gagatagcca actacctgct ggagcgcggc gcgcgtctag
     6601 atctttttgc cgccgccatg ctcggaaagc tcgatatcgt aaaagccata ttgaatgctt
     6661 atccaaacgc gatcaatttc ccagggccac atgacatttc attaatcgca cacgcccagg
     6721 ccggtggtga ggatgccagg gcagttctgg cattccttga aggtctacaa tcttaaatag
     6781 atagtcagtt tcaatgcact tgtgtcgttg aagaattcgc cgcgtaagcc gaggcaagca
     6841 atctgaggat cggtgaatac aaaaatggat gatgcagcaa ttgaagccaa cgcatcatcc
     6901 attttcagac ttaatgcaga agtcagagat cctctcaagg ttaacttgag cgttagttgg
     6961 cggccgccct catctgttgg cgcgcgatcg caacttcaaa catccgatcg aaaaacacct
     7021 tatagccacc cggatcataa aggatcatgt aatctgcggg gccatattcc tcgggcttga
     7081 acataattcc gccattggga ttgatctcca agatgaataa ctcgccctgg ttattcatac
     7141 ggatgtcaca gcgaccataa ccatttccat tcatagcctt atacattccg cgagcgagtg
     7201 tatgtaaacg ctcgatcaaa tcggggtcag tcacgcgctt gaagtcaaag ggtacatcgt
     7261 aattccactt cacgtccgta tgccagaact cctcgccagg cgggaagaca agttcggcag
     7321 gaggatacgc aattggatta ttaaaatcat cggggttgtc tacaaccaaa acattgaatt
     7381 ccttacccac gatgaactcc tccatccgtg ccgcgccgaa ttccgagcaa actcgttcaa
     7441 tctgaggcgc aagctgttca ggagtatcca ctcgtgattc gcgaaacatt cccgtgcttc
     7501 cataactctt cggatgcttc accataatcg gaaaacgaag atttttcaca agccgctcgg
     7561 cttcttcaac actcgtgacg cgatatcctc tgacaaaact gactcccttg gcttcggccg
     7621 cggcttgcat ctcctcgcgg gttgggtcaa aaaatttgga gtttgcccct gtaaatggca
     7681 ggttcatttc ctccagcgca cgtaccacat caatgccctg atatgcctgg tcatccactt
     7741 caccgtcgtt ttcgtaccct tcgcaaagat tcaggtaaat atcgaactcc ttgcgcgcat
     7801 ccagagagcg taacacctct cgcacgggat cggtcattgt gaccatctcc caatcgtacc
     7861 cctgcataaa tggtgtcggg ttgaagtcgg taatttcttc atcagacaaa acgcaaatgc
     7921 gcatcattca cctcacttcg tgtttgcagt ttttattgcg gcctggtttc gtaggatcgc
     7981 cgcgcgaaag attcggtcga gaaatcctgc atgaccgtcc ggatcatatt ccatcatgac
     8041 atcggcaggt cctaagtctt caggcttgaa taagatccca cagtttggat tgatctcaat
     8101 cagatacaat tttccatcgg gtcccatgcg gatatcacag cgagcatagc ctacgccgcc
     8161 catagccaaa tacatccgtc gggacatctc catcaaactt tgtgacaagg ctatatcgtt
     8221 gacaggcttg agatagaccc attccttcca cttcacatgc gagtgtaaaa aattctcacc
     8281 tgcggggaag atgatctctg ccggcgggta aacaaacggt tgtttcaggt cctccggatt
     8341 atcaaccacc aacacagtga attcaggccc atcaatgaat tcttccacgc gtgcactccc
     8401 aaagcgattg caaatccgtc tcacttgttc cttgagcttt gacacagcct ccacacgtga
     8461 attccgagtc attgcagtac tggcaaagct ctgagggtgc tttaccatca atgggtacat
     8521 cagactctcc accgcctgca attcatcgac gttagttata tttacgcctc gcgcaaagca
     8581 aatcccattt gattcagcca cagcctgcat ctcttctcga gacgggtcat aaaaatgact
     8641 atttgctcct gtaaacggaa gctgcaggtt ttcgagtgcg cgcacgatgt ctattccgtc
     8701 ataatcgtcc tgcggattat cagctccatc acatagattg aaataaacat cgtaatttcc
     8761 attcttctcc agcgcgcgaa caaattcagt cacgggccgt tgcgggatga aaatctccca
     8821 tacatattca tctaaatact gtgatggatc aaattccttt atttcagaat ctgacaatac
     8881 acagagttgc atgtttgtca tataagtagc tacaacgtat tatgacacaa acttttcgag
     8941 aaacaatact ttttatatta aaattttttc atagactttt cggtgcataa ttacaatgta
     9001 taatgacgaa atgtcagaac aaaaaccatc tcgccgtgct tctccaatct tcctggtcct
     9061 tggcatcgct tttcttgcga ttggacttgc tacggaccaa accgtattta catggctttc
     9121 cgttgttttt ctcgtcattt ccctcgttcg gggaggacgg tggctgagac ccagaaaata
     9181 aattactcct tcggcagttc ccctccgaaa tacgacccag cctgaactgc ttgcagaatc
     9241 gaaagcaact tagccgcgac ccttgacgcg gctattcttt tcgtctaaaa tatttaggta
     9301 agcctaaatt tcactttagc taaacattgc ggagaatatt atgagtgcaa aaattacgag
     9361 aagagatttt ctaaaggcag gcgggctgac ggtactcggc gctgtcggcg cgaatgctct
     9421 cacgaacact acatccgacc atggcaaaca ccccacgaaa gttcttgcgg cccccgccat
     9481 gcagcatgat gggcatgatg gcatcatgcc gactactgtt ggtgaagtgg atcatgagaa
     9541 gaacgggttc aatccaacag atatgctaac agatttcgat tatggcgaag tctcaaccct
     9601 gccaaatgga caggtcttgc gggaatacaa catcactgca cttaataaga acatcgaggt
     9661 cgttccggga atcgaatatc cagcatggac ttataacggt cgaatccctg gtccgacatt
     9721 gcgcgcaacc gaaggtgacc gcattcgcat caacttcagc aacggaagca atcatccgca
     9781 tacgatgcac ttccacggct ttcaccccgg caatatggac ggtgttccgg gttcgggccc
     9841 aggagggaat gttttaccag gcggatcatt cgtgtacgag tttgacgctg agccattcgg
     9901 tttacatctg tatcactgcc atgcttttcc tctcgcacgt catatcgcaa aaggac
//
LOCUS       DASTJP010000580        11272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1113273, whole genome shotgun sequence.
ACCESSION   DASTJP010000580 DASTJP010000000
VERSION     DASTJP010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11272
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113273"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFQ23_16995"
     CDS             <1..332
                     /locus_tag="VFQ23_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908348.1"
                     /translation="PVPSGMSGCVPDQIMITAPEPGAMVTGSIGIIGTADVPNFGFYK
                     YEIAPIGTQSWATISAGREAKRNEELGAWNTSSLTNGEYFLRLVVTDNQGVSLEPCVI
                     AVRVANQ"
     gene            344..883
                     /locus_tag="VFQ23_17000"
     CDS             344..883
                     /locus_tag="VFQ23_17000"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908349.1"
                     /translation="MASFDIRPAAAGDLPRMMSLDHSSLSDYVWQLELRRDTGQISAN
                     FREVRLPRSIEVRYPRNPSLLAGEWTRRDLVLVAVQDGTPIGYLCAMEDRTAAVASVI
                     DLVVSPDQRRKGAATALLMAAQTWAVERSLHRFFIEMQSKNHGCIRLAQKYGYDFWGY
                     NDQYYPTQDVALFFGRAIK"
     gene            899..2818
                     /locus_tag="VFQ23_17005"
     CDS             899..2818
                     /locus_tag="VFQ23_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908350.1"
                     /translation="MFNGWLDGLLVGLQTINNILTAGIAITAFSLFLYALSFNLRDRV
                     ARSFAIILLCVVVVFTTEALQNKSVPAWGIELLLRFQWIGIIFLPPAYLHLSDALLVT
                     AGRPSRGRRRLAVRLVYVLSAGFLILLWMGLLVGELVQDGKPAPHLQRTAWTNFFTFY
                     YIAIIVWAGYNFVRAFGLMLTRSGQRRMLYLMAGATAPALGSYPYLLFGSTIAAQNPL
                     LFWGTAFVINALVAVLVIVMAYAVAFFGVSWPDRVVKSRLFKWLLRGPVTASLALTMM
                     TLVRRAGEVFGGEPYSVFVPLVMVATVLLFEHAVTLFSPLWERALFMGRDRGEIQLLQ
                     NIEERLLTRGDLHQFLDTVLAAVRDHLQSPCAFVAALDGTELSLSVVAGNRALLEKEN
                     LEEVLQIVNGDGENGRHEFVWGDFWILPLHQRATNGDRDEVPVLLGLLGIARRADRPM
                     DKDQRDAVWLLADRAALALEDRQLQQRVFRSLEDLQPQIDMLQQMRAAGRYDTRASLS
                     PETFSQDSDVVEWVKDALTHYWGGPKLTNSPLMKLRSVQMLSEEYEGNASNALRALLR
                     KAVDQIKPEGDRRFTSEWILYNILEMKFIEGRKVREVAGRLAMSEADLYRKQRVAVEA
                     VARAILEMEQNEGIR"
     gene            2828..3580
                     /locus_tag="VFQ23_17010"
     CDS             2828..3580
                     /locus_tag="VFQ23_17010"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET9908351.1"
                     /translation="MNLGKHSDRNYENTMPAIDEGWWESVLAEEQREYRASRSMKAIP
                     PPKANNLSDTNHTKLESDIPGNSSLTDSGITSQLQSDRRAELFQSLQVGIHLTGTVSS
                     ITDFGAFVDLGGLEGLIHISELSWGRVPHPNQIVSLGEEVKVKVLEISKERNRVALSL
                     KRMSSNPWENAEREFAIDTIQSAVVSSILSYGAFARLKPGIEGLIHVSKMPLADGQTP
                     RDLLKEGQEVQVRVLYIDPAHQRMGLRLHINQ"
     gene            3580..5763
                     /locus_tag="VFQ23_17015"
     CDS             3580..5763
                     /locus_tag="VFQ23_17015"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET9908352.1"
                     /translation="MPKHSDANQIYKSEVRDDWLDRLARFNEHFGRFVRDALGVLLVA
                     AALMSILALGGYTRGALLTPWSDLLSLQFGWGAYLVAIAIGYVGFALLRRDSLAIGWS
                     RLFALELAAFLTLGLLAALGGHSLIRAEAGADGGRIGWGLITLFSWKIGQLWGTMILF
                     AIWLLALLTGLGAWAAFERWLLQLAEEPLPVETVIQPKEELETEIEKPKPEPRKKATP
                     PLPAEFRKSLKVSEHQDKKPATPRERDDRLPSLSILLADQNTRPDERTINQTAGMIEK
                     TLAEFGIVTTVIGFRIGPTVTQFAVQPGFMKRAGSTTEEEAQQIKVRVAQIASLQKDL
                     ALALSAERLRIEAPVPGKPYVGIEVPNTHTSVVRMRSILETDAFHKIGAPLAMALGRD
                     VSGQPLVADLSRMPHLLIAGATGSGKSVCITSLAACLAMNNPPEDLRMVMIDSKMVEL
                     LRFNGLPHLYGKVETNVERILGVLRWVVVEMEHRYRLLEADRARDLEAYNRKLSKKKD
                     GQPLPRIVVLIDELADLMMSAPDQTEHNLVRLAQMARATGIHLIVATQRPSTDVVTGL
                     IKANFPARLAFAVASGVDSRVILDTTGADSLLGRGDLLFLNPEVGNPIRAQGVMITDM
                     EIERLIAHWQKNADVDKSAAPWEKLLSEPDENEDEGLIEQAVSIVRQSQRASASLLQR
                     RLRIGYPRAARLLDQLEEMGVVGPSQGGGKERDVMVSPTDEDINT"
     gene            5846..6472
                     /locus_tag="VFQ23_17020"
     CDS             5846..6472
                     /locus_tag="VFQ23_17020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9908353.1"
                     /translation="MVKTVDNTTTKKTFTDYLRLWFKWVLDPIGGFLNRLGLTPNMVT
                     VLGLIGNTIGAYYLARGEMLTGGILVLIMTPIDALDGTMARLRGEPGDFGAYVDSVSD
                     RYSELIIYAGLLFYFLTLGEPNGGMLVFGAASGSVLVSYVKARAEGLGYQAKVGLLTR
                     VERYLVLAPSLVFNQLYVGLTILAVFSHITAIQRILYVRNDIHTKMKK"
     gene            6488..7294
                     /gene="lgt"
                     /locus_tag="VFQ23_17025"
     CDS             6488..7294
                     /gene="lgt"
                     /locus_tag="VFQ23_17025"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9908354.1"
                     /translation="MPDGFPLGPLYVRFYGIILMLGALAGAWLATREAKRRGHEPEIV
                     WDLLTYLLIGGIIGARIWHILTPPPSSGVTAGWYLTHPLDAMAIWKGGLGIPGAIIGG
                     AIVLYFYTRRHKLNFAEWADIASPALALGQAIGRWGNFFNQELYGSPTNLPWKIFISP
                     EHRLPEYAGVEYYHPLFLYESIWNLGNMFLLLWISRRFADRLKNGDIFLIYLIIYPVG
                     RFLLDFLRLDASLVNGININQSVMAVVAVLSVVVLYWRHREDISARQRLA"
     gene            7370..8308
                     /locus_tag="VFQ23_17030"
     CDS             7370..8308
                     /locus_tag="VFQ23_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908355.1"
                     /translation="MIETYDLSKQFNHDFWAVDGVTLKVEPGQILALLGQNGAGKTTT
                     VRMLTALLKPTRGWARVGGYDITRNPQEIRTKVGVLTEQHGLYMRMSGAEYLDFFGEI
                     YKLDPKIRKSRSDYLLDYFGLTEAAHRRSGEYSKGMKQKLALARALMHDPTVLLLDEP
                     TSAMDPESAQLVRNEISRLRSSQRTIIICTHNLAEAEALADRIAIIYRGKILLNGTLD
                     ELKRDVLGPVEYEAIFAEAFDVGDIVLPSGVTLSAQNATSLRFRVEEPQAANPKLVHL
                     LTSRNAPLVSFQEVPRKLEQVYLKTMADAQGVTYAA"
     gene            8298..9734
                     /locus_tag="VFQ23_17035"
     CDS             8298..9734
                     /locus_tag="VFQ23_17035"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET9908356.1"
                     /translation="MRHNLSTIWMVARREFIDQFRDWRIIVPMLLLVTLFPFIADDTT
                     RQAVNFMNRFGGDLILDNLIPFVVLVIGFFPLSFTLVVALESFVGEKERGTIEPLLSS
                     PLEDKHMYLGKLLVGITTPLVFSFVSIGIYLYLVSRREIEFPGAYMLALIFLLTFAHA
                     VLMVSSAIVISVQATTIRAANLLASFVVVPVAFLLQGETILIFWGNEDVLWFAIAGVT
                     LLSGLLVRLGLSHFRREYLLGREIDTLNLKWMWRAFRNRFTGEATSISEWYTRELPLT
                     LRQLKQPLMIVIALALVGGIASYLWVIVNVPAYVNLSPDQVTEIRTFLAENLANIDRL
                     GQQLPAPVLFFHNARTTVVFLLLGLVSFGTLGLTLFIVNIGLVGGLLGAASLVGYSPL
                     AVFAVGILPHGIFELTAVFLATAAMLKTGAQLVTPQADKSLGELLLISLADWFRIFIG
                     IAIPLLAIAAVIEIYITPRLIKEFFPFL"
     gene            9748..10503
                     /locus_tag="VFQ23_17040"
     CDS             9748..10503
                     /locus_tag="VFQ23_17040"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9908357.1"
                     /translation="MPKVIILGSSNAVPTKDHENTHLVIVGEQRTILVDCVSTPVIRL
                     EQAGVDFNDLTDIVITHFHPDHVSGVPLLLMDMWLMGRTKPINIYGLHYTMDRLEDLM
                     GFYGWEEWPNFFPVGFYRLPESEMAHVLDCSEFKIRSSPVHHMIPTIGLRVECKNPSK
                     VLAYSCDTEPCEQTVRLAEGADILIHEAAGATFGHSSAEQAGEIATKAEVGKLYLIHY
                     PSGRFAKGDIALEASKTFKGEIIVAKDFMTIDI"
     gene            complement(10547..11050)
                     /locus_tag="VFQ23_17045"
     CDS             complement(10547..11050)
                     /locus_tag="VFQ23_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET9908358.1"
                     /translation="MHEPVLVLNANFEPINVCTTRRAIGLLLAGKADMVANGRGHIRT
                     VKQLIPRPSVIRLEMQIHRPRPHVKLTRREVFRRDNYTCQYCGKREGGLTVDHVIPRH
                     LGGEHVWINVVAACPHCNHRKGGRRVEEAHMRLLHIPKEPPASAMYIFGRHIEEYNEW
                     APYINGW"
BASE COUNT         2581 a         2660 c         2994 g         3037 t
ORIGIN      
        1 agcccgttcc cagcggtatg agcggttgcg tacctgatca gatcatgatt accgctccag
       61 aaccaggggc gatggtgacc ggaagtattg gaatcattgg cacagctgac gttcctaatt
      121 ttggtttcta taaatacgag attgctccga taggtactca aagctgggca accatttccg
      181 cgggtcgaga agcaaagcga aatgaagaat tgggcgcatg gaatacctct tcgctaacca
      241 atggggaata ctttctgcgc ctggtggtta ccgacaatca aggtgtatct ctggaaccgt
      301 gtgtgatcgc ggtgcgcgta gcaaatcagt gattggagga aaattggctt cttttgacat
      361 tcgtcctgcc gcagcgggtg atctgcctcg tatgatgtcg ctggatcact ctagtttgag
      421 cgattacgtc tggcagttgg aattgcgccg cgacactggt cagatctctg ctaatttccg
      481 cgaggtaagg ctgccgcggt cgattgaggt tcggtatccg cgcaacccgt ctttgttagc
      541 tggtgaatgg acaaggcgcg atctggtatt ggtcgctgtg caggatggca cccccattgg
      601 ctacctgtgt gcaatggagg atcgaacggc tgccgtggct tcagtcatcg acctggttgt
      661 ctcacctgat cagagacgta aaggtgcagc tacagctctc ttaatggcgg cccagacctg
      721 ggccgtggaa cgaagcctcc accgtttctt catcgagatg cagtcgaaaa accatggttg
      781 tattcgcctt gcacaaaaat acggctatga tttctggggt tataatgacc agtattaccc
      841 aacacaggac gtagctttat tttttgggag agccataaaa taatattgga aggtttgcat
      901 gtttaatggt tggttggatg gcttgctggt gggacttcaa acaatcaata atattttaac
      961 agccggtatt gcaatcacgg cgttttccct tttcctctat gccttatctt tcaatttgcg
     1021 agatcgtgtt gcacgttcct ttgcgatcat actgctttgt gtagttgttg tctttacgac
     1081 cgaagcactc caaaataaat cggtgcctgc ctgggggatt gagttgcttc tacgattcca
     1141 atggatcgga attatctttt taccacctgc ctatttgcat ctttctgatg cattacttgt
     1201 cactgccgga cggccctcgc gcggaaggcg acgattagcc gttcgtttgg tgtatgtatt
     1261 atctgctggt tttctaattc tgctctggat gggcctactg gtaggggagc tggtacaaga
     1321 tgggaaaccg gccccacact tacagcgcac cgcctggaca aactttttta ccttctatta
     1381 catcgcaatc atcgtgtggg cgggatataa ctttgtccgc gccttcggcc tcatgctgac
     1441 tcgttcaggt caacgaagaa tgttgtatct gatggccggt gcgacagcac cagccctggg
     1501 atcctatcca tatctcctgt ttggttccac aattgccgcg cagaatccgt tgctgttttg
     1561 gggcacagcc ttcgttatta atgcgctggt ggcagtcctg gtaatcgtga tggcatatgc
     1621 cgtagctttt tttggcgtgt cctggcctga tcgggtggta aagagccgat tgtttaagtg
     1681 gttgctgcga ggtcctgtga ctgcctcgtt agcactaacc atgatgaccc tggtgcgccg
     1741 cgccggtgag gtctttggcg gcgaacccta ttcggtattt gtcccacttg tcatggtggc
     1801 aactgtttta ttgtttgaac acgctgtgac cttattttca ccattgtggg aacgggcctt
     1861 atttatgggt cgtgaccgtg gtgaaatcca actccttcaa aatatcgaag aacgattatt
     1921 gacgcgcgga gacttacatc aattcctgga tacagttctg gcagccgtcc gcgatcattt
     1981 acagtcaccc tgcgcatttg ttgctgcact ggatggaaca gaattatcgc tcagcgttgt
     2041 tgccgggaat cgagcattgc ttgagaagga aaatttagag gaagtcctgc aaattgttaa
     2101 cggcgatggt gaaaatggaa ggcatgaatt tgtctggggc gatttctgga ttttgccgtt
     2161 gcatcagcgc gcaaccaacg gtgatcgcga tgaagttcct gtgttattgg gcttgcttgg
     2221 aatagctcga agagcagatc gccccatgga taaggatcag cgcgatgcag tctggcttct
     2281 tgcggatcgc gccgcactcg cgctggaaga tcggcagtta cagcagcggg tctttcggtc
     2341 attggaagat ttgcagcctc aaattgacat gcttcaacaa atgcgtgccg ctggtcggta
     2401 tgataccagg gccagcctct cgcctgagac gttttcgcag gattccgatg ttgtggagtg
     2461 ggtgaaagat gctctcacac attattgggg cggtccgaaa ttaacaaaca gtccgctgat
     2521 gaaacttcga agcgttcaaa tgctttcaga agagtatgaa ggaaatgcat ccaacgcctt
     2581 gcgagctctc ttgcgcaagg ctgtggatca aatcaagccc gaaggggatc gccgttttac
     2641 cagcgaatgg atcctctata atattctcga aatgaagttt attgaaggac ggaaagtccg
     2701 cgaagtcgct gggaggctgg caatgtcgga agccgacttg tatcgcaaac agcgcgtggc
     2761 cgtggaggcc gtggcgcgtg ccattctcga aatggagcaa aacgaaggaa ttcgataagg
     2821 aggaaatatg aatcttggta aacactccga ccgcaactat gagaatacca tgcccgcaat
     2881 tgatgaggga tggtgggaat cggtcctagc ggaggaacaa cgcgaatatc gcgcatcgcg
     2941 aagtatgaaa gcgattcctc caccgaaggc aaataacctt tcggatacaa atcacacaaa
     3001 gctggaatca gatatccccg gcaattcgtc attaaccgac tcaggaatca cgtctcaact
     3061 gcaatcagat agacgggccg aattatttca aagtcttcag gttggcattc atctgacagg
     3121 cactgtaagc agtattacgg actttggcgc tttcgttgat cttggaggcc tggaagggtt
     3181 aatccatatt tcagagcttt cctggggacg tgtccctcat ccgaatcaaa ttgtcagcct
     3241 gggtgaggag gtcaaggtca aggtgttgga aatctctaag gagcgcaatc gagtagccct
     3301 gagcttgaag cgtatgagtt cgaatccctg ggagaacgct gaaagagagt ttgctatcga
     3361 tacgatccaa tctgcagtgg tatccagtat attatcctat ggcgcgtttg cacggctgaa
     3421 acctggcatt gaaggtttga ttcatgtttc taaaatgcct ctggccgatg gacaaacccc
     3481 gcgtgacctt cttaaagaag gacaggaagt tcaagtgcgt gtgctatata tcgatcctgc
     3541 tcatcaacgt atgggattaa ggttgcatat caatcaataa tgcctaaaca ttcggatgca
     3601 aatcagattt acaaatcaga agttcgcgac gactggctgg accgcctggc ccgtttcaat
     3661 gaacactttg gtcgtttcgt tcgcgatgcc ttgggtgtgc ttttggtcgc tgcggctctc
     3721 atgtcgattc ttgctctggg aggttatacg cgcggcgcct tgttaacgcc ctggtcggat
     3781 cttttgtctc tgcaatttgg atggggggca tatcttgttg ccattgctat cggatatgtc
     3841 ggatttgccc tgcttcgacg cgacagcctg gcaatcggat ggagtcggtt atttgcgttg
     3901 gaactagctg catttctgac cttgggcctg cttgccgccc tgggaggcca ttctctcatc
     3961 cgcgccgagg ctggagcgga tggtggtcgg attggctggg gtttgatcac cttattttcc
     4021 tggaagatcg gtcaactctg gggaaccatg attctctttg caatttggct tcttgcctta
     4081 ctgaccggtt tgggcgcctg ggctgcattt gaacgatggt tgttgcagct cgcagaagag
     4141 cctctacctg tagaaactgt aatccagccc aaagaggaac tggaaaccga aatcgaaaaa
     4201 cctaagcctg agccacgcaa aaaggccacg cctcccttgc cagccgagtt tagaaaatct
     4261 ctgaaggttt cagagcatca ggacaaaaag ccagctacgc cgcgcgaacg tgatgaccga
     4321 ttgccatcac tgagtatcct gctggcagat caaaacacac gtcccgatga gcggacaatc
     4381 aaccaaactg ctggcatgat cgaaaaaacc ctggctgaat ttggcattgt cacaacagtg
     4441 atcggttttc gtatcggacc cacagtgact cagttcgcag tgcagcccgg cttcatgaag
     4501 agagctggta gcacgactga ggaggaagcg caacaaatta aagtacgtgt tgcgcaaatt
     4561 gcttcacttc aaaaagacct tgcacttgct ctttctgcag aacgcttacg aatagaagcc
     4621 cctgttcccg gtaaacctta tgtgggcatt gaagtaccaa acacgcacac gtccgtcgtg
     4681 cgcatgcgtt cgattttaga aacggatgcc tttcataaga tcggtgctcc gcttgcgatg
     4741 gccctggggc gcgacgtttc gggccaaccc ttagtagctg atttgtcgcg tatgcctcac
     4801 ctcttgattg cgggagcaac cggttcaggt aagtccgtat gcatcacatc tctcgcggcc
     4861 tgtcttgcaa tgaacaaccc gccggaagat ttacgcatgg tgatgatcga ttccaagatg
     4921 gtggaactac tgcgttttaa cggtttgcct catctttatg gaaaagtgga gacaaatgtt
     4981 gaacgcattc tcggtgtgtt acggtgggtg gtcgttgaaa tggaacatcg ctatcgactg
     5041 ctcgaagctg accgtgcgcg cgaccttgaa gcgtataatc ggaagctttc aaagaaaaag
     5101 gatggacagc ccctgccgcg catcgtcgta ttgatcgacg agttggccga tttgatgatg
     5161 tctgcacctg accaaaccga gcacaacctg gtccgtcttg cccagatggc gcgtgcaaca
     5221 gggattcatc ttattgtcgc cacacaacga ccctcgaccg atgttgttac cggtttgatc
     5281 aaagcgaatt tcccagctcg cctggctttt gctgtggcat ctggtgtcga tagccgcgtc
     5341 atcctcgata cgacgggggc agatagcctg ttggggcgtg gcgatctgct tttccttaat
     5401 cctgaggtag gtaaccccat ccgcgcgcag ggtgtgatga tcaccgatat ggaaattgag
     5461 cgcctcattg cgcactggca gaagaatgcc gatgtggata agtcggcagc tccctgggaa
     5521 aagctattat cagaaccaga tgaaaacgaa gatgaaggcc tgattgagca ggcagtctct
     5581 atcgttcgtc agagtcagcg cgcctctgct tccttactgc agagacggtt gagaatagga
     5641 tatccgcgtg cagcgcgctt gctcgatcaa ttggaagaaa tgggggttgt gggtccatct
     5701 cagggtggcg ggaaggaacg tgacgtaatg gtgagcccca cagatgagga tattaataca
     5761 tagtaagtca attacaaatt gaagaaatca attcgaacgc cgttttgctg ttatcattgc
     5821 ctgacgataa ggacaaaggc atttgatggt aaagactgtc gataatacaa ctacgaaaaa
     5881 aacatttacg gattatctgc gcttgtggtt caagtgggta cttgatccca ttggcggatt
     5941 cttaaaccgt cttgggttga cccccaacat ggtgaccgta ttggggctga ttgggaatac
     6001 gattggcgca tattaccttg ccagggggga gatgttaacg ggtggtattc tggtcctgat
     6061 tatgacgccc attgatgctc tggacgggac aatggctcgc ttgcgcggag agcccggtga
     6121 tttcggagcg tatgtggatt cagtatcgga tcggtactct gaactgatta tttacgcggg
     6181 tcttttattt tacttcctga ctttgggtga gccaaatggc ggtatgcttg tctttggagc
     6241 agcctccgga tcggtgctgg tttcttatgt aaaggcccgg gcggaagggt taggttatca
     6301 ggccaaagtc ggcctgttga ctcgtgtgga gagatatctc gttctcgcgc catccctggt
     6361 cttcaaccaa ctctatgttg gtctcaccat tcttgcagtg ttttcccaca tcactgccat
     6421 acagcgtatt ttgtatgttc gaaatgacat acatacaaaa atgaaaaaat aagtttggag
     6481 atatcacatg cccgatggat ttcccctcgg tccgctttac gttcgattct acggaatcat
     6541 cctcatgctt ggcgcgcttg ccggcgcatg gctcgccact cgagaggcca agcgccgcgg
     6601 gcacgaacct gagatcgttt gggacctgct gacctacctg ttaattggtg ggattattgg
     6661 agcacgaatc tggcatattc ttaccccgcc tccctcatca ggagtgacag ccggttggta
     6721 tctcactcat ccgctggatg cgatggcaat ctggaaaggc ggcctaggga ttccgggcgc
     6781 gatcattggt ggtgcgattg ttttatattt ctatactcgc cgtcacaaat taaatttcgc
     6841 ggagtgggca gacatcgcgt cacccgcctt ggcactgggg caggcaattg gaagatgggg
     6901 aaattttttt aatcaggaac tctatggcag tccgacaaac ctgccctgga aaatctttat
     6961 cagccctgag catcgactgc ctgaatatgc aggcgtcgaa tactatcatc cgctattttt
     7021 atatgaatca atttggaatt tgggcaatat gttccttctt ttgtggattt cccgtcgatt
     7081 tgcggatcga ctcaagaatg gagacatctt tctcatttac ctgattattt atccggtcgg
     7141 gcgtttcctg ctggattttc tgcggctcga cgcttcgctg gtgaacggta ttaacatcaa
     7201 tcaatcggtc atggccgtgg ttgccgtcct ttcagtggtt gtattgtatt ggcgtcatcg
     7261 cgaagacatc tcagcgcggc agcgcctggc ataatttgta aggactgttg tcttattgac
     7321 cctttcattg gatatgctat gctcggctgg gtgaccttta ggaaccataa tgatcgaaac
     7381 atatgatttg agtaaacaat tcaatcacga cttctgggcg gtcgatggcg tcaccttgaa
     7441 ggtagaaccg gggcaaattt tggcgttgct tggtcaaaac ggagcaggca agactactac
     7501 tgttcgtatg ctaactgctt tactgaaacc tacgcgaggc tgggcgcggg ttggcggata
     7561 cgacattacg cggaatccac aggaaatacg tacaaaagtg ggggtactta ccgaacaaca
     7621 tggcctttac atgcggatga gcggcgccga gtatttggat ttcttcgggg aaatttataa
     7681 gcttgacccc aaaataagaa aatctcgtag cgattactta cttgattatt ttggtctaac
     7741 tgaagctgca caccggcgct ccggtgaata ctcaaaaggt atgaagcaga aattggcgct
     7801 ggcgcgcgcg ctcatgcatg atccgacggt tttgctcctc gatgagccca cctcagccat
     7861 ggatcctgag tctgcacaat tggttcgcaa tgagatatcg cgtttgcgct cctctcaaag
     7921 aaccatcatc atctgtacac ataatcttgc cgaagcagag gctcttgccg ataggattgc
     7981 gatcatctat cgcggaaaaa tcttattgaa tgggacattg gatgaactaa agagggatgt
     8041 attgggacct gtcgagtatg aggcaatatt tgcagaagca tttgacgtgg gcgacatagt
     8101 cctcccttcc ggcgtaacac tgtcggcgca aaacgcgacc agtttacgtt ttcgggtgga
     8161 agagccgcag gctgcaaacc ccaagcttgt acatctgctc acatctcgca atgcgcccct
     8221 ggtttccttt caggaagtcc cgcggaaact ggagcaggtc tacctcaaga ctatggccga
     8281 cgcccagggg gtgacgtatg cggcataatc tgagcacgat ctggatggtg gcgcgtcgtg
     8341 aattcattga tcagttccgc gactggcgta ttatcgtgcc gatgctgttg cttgtgactt
     8401 tattcccgtt catcgccgat gatactaccc gacaggccgt gaatttcatg aatcgatttg
     8461 ggggcgacct gatcctggat aacctgattc cgtttgtcgt gttggtcatc ggtttcttcc
     8521 ctttgtcttt caccctggtc gtggcacttg agtcgttcgt gggtgagaaa gaacgcggca
     8581 ccattgagcc actgctgagt tcgccgcttg aagataagca catgtatcta ggtaagttgc
     8641 tggtgggaat cacaacgccg cttgtcttca gcttcgtttc gattggtatt tatttgtatt
     8701 tggtttcacg ccgtgagata gagtttcctg gcgcctacat gctggctctg atctttttgc
     8761 tgacatttgc gcatgcagtt ctcatggtca gttctgcgat cgtgatctcg gtacaggcca
     8821 ccacaattcg cgctgcaaat ttgctggctt ccttcgtcgt tgtaccggtg gcattcctgc
     8881 tccagggtga gacgattttg attttctggg gcaatgaaga tgtgttgtgg tttgccattg
     8941 ccggggttac gctgctgtcg gggttgctag tgcgtttggg actgtcgcac tttcgccgtg
     9001 aatacctgct tggacgcgag attgatacat taaacctaaa atggatgtgg cgcgcgttcc
     9061 gtaatcggtt caccggggaa gccacttcca tatccgagtg gtatacccgg gaattgccgc
     9121 tcacgttgcg acagctaaag cagcctttaa tgatcgtaat tgctcttgcc ttggttgggg
     9181 gaatcgccag ctacctgtgg gtgatcgtga acgtgcctgc ttacgttaat cttagtccag
     9241 accaggttac cgagatcagg acatttctgg cagagaatct cgcaaatatc gatagactag
     9301 gtcaacaatt acctgcgccg gtcttatttt ttcataatgc ccgtacaacg gttgtatttc
     9361 tcttattggg gcttgtttca ttcggtacgc tgggattgac cttgttcatt gtgaatatcg
     9421 gactcgttgg cggcctgctc ggtgccgcaa gcctggttgg gtactcgcct ttagctgttt
     9481 ttgccgtggg gatcttgccc catggcattt ttgagttaac cgcggttttt cttgcaactg
     9541 cggcgatgtt aaagacgggt gcgcagctcg taacacccca ggcagataaa agcctgggcg
     9601 aattgttgtt aatttcactt gcagattggt tcaggatttt tatcggcatt gccatccctc
     9661 tccttgcaat tgcagcagtg attgaaattt acattacccc ccggttaatc aaagagtttt
     9721 ttccgttttt ataaataaag agggcgtgtg ccaaaagtca tcatacttgg ttcatccaac
     9781 gcagtgccga ccaaagacca tgaaaatacc catctggtga tagtggggga acagcgcacg
     9841 atccttgtcg attgtgtcag cacaccggtc atcaggctcg aacaggcagg cgtcgatttc
     9901 aacgatctta cggatatcgt catcacacat tttcatccgg atcatgtgtc tggggttcct
     9961 ctgcttctga tggatatgtg gctgatgggt cgtacgaagc cgatcaacat ctatggtttg
    10021 cattacacaa tggaccgttt ggaggatcta atgggttttt acggttggga ggagtggccc
    10081 aattttttcc cggtcggctt ttatcgtctt ccagaatcag aaatggcaca tgtattagat
    10141 tgctccgaat tcaaaatccg ttcctctcct gtccatcata tgattccgac gattggtctg
    10201 cgcgtggagt gtaagaatcc cagcaaagtt ctcgcttatt cctgcgatac ggagccatgt
    10261 gaacaaacgg ttcggctcgc ggagggagca gatatcctga tccacgaagc ggccggggca
    10321 acctttggac attcctctgc ggaacaggca ggtgagatcg cgacaaaagc cgaggttggt
    10381 aagttatatt tgatccatta tccatcaggg cgatttgcga aaggcgatat tgcgctcgaa
    10441 gccagcaaga catttaaagg cgaaattatc gttgccaaag atttcatgac gattgatatt
    10501 taaataaatt cccctctcca accaggagag gggctagggg tcatttctac cagccgttaa
    10561 tatacggcgc ccattcgtta tattcttcaa tgtgcctgcc aaaaatatac atggcgctcg
    10621 ccgggggctc tttaggaata tgaagcaagc gcatatgagc ctcctccact ctccgtccgc
    10681 ctttgcgatg attgcaatgc gggcaggccg ctaccacatt gatccataca tgctcaccgc
    10741 ctaagtggcg agggatgaca tggtccaccg tcaagccgcc ctcgcgcttc ccgcagtact
    10801 ggcaggtata attatcgcgg cgaaatacct cacggcgagt taacttgaca tgagggcggg
    10861 gccgatgaat ttgcatctcc agccgaatga ctgaggggcg ggggatcaac tgtttcaccg
    10921 tccgaatatg cccacgtccg tttgccacca tatctgcctt acctgccagc agcaaaccga
    10981 tggctcgccg cgtggtgcac acattaatag gctcaaaatt ggcgtttagc accaaaacag
    11041 gctcgtgcac acggcctcca taatagggac gattatactg ctgaattttt gaattcgtaa
    11101 attgtgcccc agggagtgtc atgaacttgt cataggagtt ggtgatgaag gtaataatag
    11161 caggcggttc aggcttcctt gggagggcgc tcacaaattc tctcaggcgg gatgatcacg
    11221 aagtattgat tctcactcgc cgcgctcgaa aatccactgc tgaattcgaa tg
//
LOCUS       DASTJP010000581         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1418009, whole genome shotgun sequence.
ACCESSION   DASTJP010000581 DASTJP010000000
VERSION     DASTJP010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418009"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            598..1950
                     /gene="dnaA"
                     /locus_tag="VFQ23_17050"
     CDS             598..1950
                     /gene="dnaA"
                     /locus_tag="VFQ23_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9908359.1"
                     /translation="MNVEQAWQSVLGQLQMEMPRASFDTWVRDTKPLSYQDGTLTIGV
                     RNAYARDWLESRLASTVSRLLVGIMNASVAVNFIVNGNDAEVIADEASVSSVQPESAP
                     VFEQPVRPSNMNPRYLFETFVVGSGNRLAHAACLAVAEKPARAYNPLFLYGGVGLGKT
                     HLLHAIGNTCHARGLNVLYVSSEEFTNDMINAIRTHTNQAFREKYRSADVLLVDDIQF
                     IAGKESTQEEFFHTFNTLHGQDKQIIVSSDRPPKSLNTLDERLRSRFEWGLTADIQPP
                     DLETRLAILRSKAERTGRQVPDDILESIARRVQSNIRELEGALNRILAFADLSGTHLS
                     SQLVEVALADLLPQRSDVQPEKIIELVAKEWQTSVAALLGRDRSQKIAQPRQVAMYLL
                     RKETDASLPQIGEVLGGRDHTTVMYAIEKIASEIETKTDLRKRVVNIKQQLYGQGAVV
                     "
     gene            complement(2006..3331)
                     /locus_tag="VFQ23_17055"
     CDS             complement(2006..3331)
                     /locus_tag="VFQ23_17055"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HET9908360.1"
                     /translation="MDRLLEMSSKELNRFEVMQRLSKTQMSQKEAGTILRLSVRQIKR
                     LLKAYRHKGAAGLVSKHRGRKGNNRLSEEVKKRALNLLKTKYRGFGPTLAHEKLVEKE
                     NLKLSDESVRQLMIAEDLWKARKAKKETVHQLRERRACFGELVQIDGSPHDWFEGRAA
                     ACVLLVFIDDATGELVQLLFVESESFFSYCQAAEGYFKRCGKPVAFYSDKNSIFRVNV
                     PSVGSGDALTQFGRAMLELDIEIICANTPQAKGRVERVIQTLQDRLPKEMRLRGIHSH
                     ETGNAYLPEFIQDFNQRFREEPRSDVNAHRPLTAKEDLARILTWQETRSLSKNLTLQF
                     YKTVYQIQTKRPSYALRKAQVTVCLNAQEQITILYNGKSLPYAIYHSQAKQADIVPAK
                     LLDQALQKPRLPTQPAPDHPWRQGFATPLSKRGNATFTRKGDISILENR"
     gene            3562..4626
                     /locus_tag="VFQ23_17060"
     CDS             3562..4626
                     /locus_tag="VFQ23_17060"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9908361.1"
                     /translation="MLKLPGLIDPHVHVREPGQIHKEDWDSATRAALAGGVTMILAMP
                     NTRPPIFDAATLALALEAARQKAHCDYAQFVGAGPDNADLLVPLASRAAGMKMYLDST
                     FGELRLDDMTLWMPHFANYPKEYPVVLHSESRTMAAGILFAALYDRPVHIAHISLQEE
                     ILLIKAAKERGIKVTCEVCPHHLFLSKDDIPAVSSGHPGRGEVRPRLATREDVNSLWE
                     NMDVIDCFATDHAPHTLEEKDSENPPPGFPGLETLLPLLLTAVDDGRLTIEDVFAKSV
                     INPGKIFNLPEQPGTWIEVEEDAKYEIHASDQFTRCGWTPFEGWKVRGKIRKVVLRGK
                     TVFEDGKILAEPGYGENVRK"
BASE COUNT         1112 a         1245 c         1273 g         1148 t
ORIGIN      
        1 catcggacat gggcttgcca acatgcagac gcgtgcgcgt gcagttggcg gtgatgtgga
       61 tatttcatcc gtgatcggtg agggaacgac cgtgcttgca tgggttccgc gttgatcgca
      121 gagcgaaatg tatggaatca aaaattgttc cgtatcgaat cacagaatcc tgagtctcgc
      181 caatcaagaa acgaaaagtt ttgatcacgc agttctttaa gaagagacat ccatatttat
      241 ctcaaagaag tttaaatcgg gattaaagca ccatatatgg gggtcgcaaa ttttcatcag
      301 gttgtacttg gctgaaacag gtgttctatg gcaaatcgtg atttgctccg tctccccgcg
      361 atgaatcttt aacattgagt gaccttaaaa aagacgaagt tttaaaatta gcgtaggctt
      421 tccgtgttgt tatttccatg ttcctctgct agaatggaaa cacgctgaga tataaggatg
      481 tggtgatcat accccatcgt aagccccaat aaatctcata acaatttaaa agtactacag
      541 aatgagccgc tgataaggcg ggttaattcg acattctctg ttaccagtgg agcagctatg
      601 aacgttgaac aggcatggca atctgtactc gggcaacttc aaatggaaat gccccgcgcc
      661 tcttttgata catgggtgcg tgacaccaaa ccactttcct accaggatgg gaccctgacc
      721 atcggagtcc gtaacgcata tgcgcgcgac tggttggaaa gtcgtctggc gagtacggtc
      781 agccgtttgc ttgtggggat catgaatgcc agtgttgcgg tgaacttcat cgtgaatggc
      841 aatgatgcgg aagtcattgc cgatgaagcg tccgtttctt cggtgcagcc ggagtcggcc
      901 ccggtttttg agcagccagt gcggccatcg aacatgaatc cgcgctacct gtttgaaaca
      961 tttgtggttg gctccggcaa ccggctggca cacgcggcct gcctggccgt ggcagagaag
     1021 ccggcacgcg cctacaatcc gttattcctg tacgggggtg tgggactggg aaagacccac
     1081 ctcctgcatg cgattgggaa tacctgtcac gcccgtggcc tgaacgtttt gtatgtctcc
     1141 tccgaagaat ttaccaacga catgatcaat gcgatccgga cgcatacgaa ccaggcgttc
     1201 cgcgagaagt atcgctcggc agacgtgctg ctggtggatg atattcaatt cattgccggc
     1261 aaggaatcca cgcaggagga gtttttccac acgttcaata ccctgcacgg gcaggacaag
     1321 cagatcatcg tttcctcgga tcgcccccca aaatccctta atacactcga tgaacgcttg
     1381 cgctcgcggt tcgaatgggg actcaccgcg gatatccagc cgccagatct cgaaacccgc
     1441 cttgccattc tgcgctcaaa agcggaacgg accggtcgcc aggtgccgga cgatatcctg
     1501 gagagtattg cccgtcgtgt gcagtccaat atccgtgagc tggaaggcgc cttgaatcgc
     1561 atccttgcct ttgccgatct cagcggcacg cacttgagct cccagcttgt cgaagtggca
     1621 ctggcggacc tgctgcccca gcgcagtgac gtccagccgg aaaagatcat cgaattggtg
     1681 gcaaaggaat ggcagacgag cgtcgcagct ttgctcggtc gcgaccggtc gcagaagatc
     1741 gcccagccgc gtcaggttgc catgtacctt ctccgaaaag agacggacgc ctcattgccg
     1801 cagattggcg aagtgctggg gggtcgcgac cacacgaccg tgatgtatgc catcgagaag
     1861 atcgccagcg agatcgaaac caagacagac ttacgcaaac gcgtggtcaa catcaagcag
     1921 cagttgtatg ggcaaggcgc ggtggtctag tggggcgagt ccgtaaaccg agcgcaagtc
     1981 ggtgtgttag cccaaagtta aaatgtcacc tattttccaa aatagaaatg tccccttttc
     2041 gggtaaaggt ggcatttcca cgtttggaga ggggcgtcgc aaagccttgg cgccagggat
     2101 gatccggagc aggttgggtt ggcagccgtg gcttctgaag agcctggtcc agtagcttag
     2161 cagggacgat atcagcctgc ttcgcctgcg agtggtaaat ggcgtaaggc aaagatttgc
     2221 cattatacaa gatcgtaatt tgctcctggg cattgagaca aacagtgacc tgggccttac
     2281 gtaaggcata ggaggggcgt ttggtttgga tctggtaaac ggtcttgtaa aactgcaggg
     2341 tcagattttt ggaaagagaa cgtgtttctt gccaggtaag gatgcgggcg agatcttctt
     2401 tcgctgtcag cgggcgatgg gcattcacat cgcttctggg ttcttcgcga aagcgttggt
     2461 tgaaatcctg gataaattca ggcaagtagg cattgccagt ttcatggctg tggatgccac
     2521 gcaagcgcat ttctttcggc aggcgatctt gcagcgtttg aatgacccgt tcgacgcggc
     2581 ctttggcttg cggggtattg gcacagatga tctcgatatc cagttcgagc atggcgcgtc
     2641 cgaattgggt cagagcatcg ccagaaccaa cggagggcac attgacccgg aaaatgctgt
     2701 ttttgtcgct gtagaaagcc acgggcttgc cacagcgttt gaaatagccc tcggcggcct
     2761 ggcagtagct gaagaagctc tcgctctcca caaagaggag ttggactaac tcgcccgtcg
     2821 catcgtcgat aaacaccagc aggacacaag cggcggctcg tccttcaaac cagtcatgcg
     2881 gcgacccatc gatttgcacc agctccccaa agcaggcgcg ccgttctcgc agttgatgca
     2941 ccgtctcttt cttggctttg cgggctttcc acaaatcttc tgcgatcatg agttgccgca
     3001 cactttcatc cgagagtttg aggttctcct tctccaccaa cttctcgtgt gccaacgtcg
     3061 gtccaaagcc acggtatttt gttttcagta aattcaaagc tcgcttcttg acttcttccg
     3121 ataaacgatt attccctttc cgcccgcggt gcttggacac caagcccgcg gcgcccttgt
     3181 gacgataggc cttcagtaag cgcttgattt ggcgcacact caagcgcagg attgttcctg
     3241 cttccttttg gctcatttgc gttttggata atctttgcat cacttcgaat ctgtttagtt
     3301 cttttgagct catctcgagt agtctgtcca tttcttgtcc ctttccaaag gggacatttt
     3361 aactttggta gaaagggaca tttctacttt gggctaacac gtgaataccc tgcccttgac
     3421 aaagaacccg ctctgcgtag aatcacgcag acatgaccaa tcgcttcttt cgagaccctc
     3481 tgccggttac cgtcaaatta acgtttcctt ccccgattta ttaagagagc cgccatcggc
     3541 tctttttttt tatacttttc tatgctcaaa cttcccggct taattgaccc tcacgtccac
     3601 gtgcgcgaac ctggacagat ccacaaggag gattgggatt ccgccacccg ggctgccctc
     3661 gccggcggtg tgaccatgat cctggcgatg cccaatacca ggcccccgat cttcgacgcc
     3721 gcgactctcg ccctggctct cgaggctgcg cggcaaaagg cgcactgcga ctacgctcaa
     3781 tttgtgggcg caggtccaga caacgcagat ctcctcgtac ctcttgcttc ccgggccgct
     3841 ggaatgaaga tgtacctcga ctctaccttc ggcgagctcc gcctcgatga tatgactctc
     3901 tggatgccgc acttcgcaaa ctacccaaaa gaatatccag ttgtcttaca ttccgaatcc
     3961 cggaccatgg cggcaggaat cctcttcgcc gcgctgtatg atcgccctgt tcacatcgcg
     4021 catatttctc tgcaggagga aattttactg atcaaagctg ccaaggaacg cggcatcaaa
     4081 gtgacgtgcg aggtctgccc gcatcacctc ttcctctcca aagatgatat tcctgctgtc
     4141 agcagcggac atccggggcg cggggaagtg cggccgcggc tcgcgacaag ggaagatgtc
     4201 aattcgttat gggaaaacat ggatgtgatc gactgttttg ccaccgacca tgccccccat
     4261 acgctggaag aaaaggactc cgagaatcct ccacccgggt ttccaggcct ggaaacgtta
     4321 ttgccgcttc tgctcaccgc agtggacgat ggacgattga ccattgagga tgtttttgcg
     4381 aaatcggtga tcaaccccgg aaaaatcttc aatcttccgg agcagcccgg cacatggatt
     4441 gaagtggagg aggatgccaa atacgaaatc catgcatcag accaattcac ccgctgcggc
     4501 tggactccct tcgaaggctg gaaggtaaga ggcaaaatca gaaaagttgt gctgcgaggt
     4561 aaaacagtct ttgaagatgg aaaaatattg gcggaacctg gttatggaga gaatgtaaga
     4621 aagtaggaga aatggaagat tttaccgatg tgaaacttga aatctttgtc ccacaggaat
     4681 atgcgctcag ggtccgtgat caacttgcaa agattggcgt cggacgcatc ggaaattacg
     4741 atcactgtgt tgcgatctac ccggtgcagg gatacttc
//
LOCUS       DASTJP010000582         8163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1418021, whole genome shotgun sequence.
ACCESSION   DASTJP010000582 DASTJP010000000
VERSION     DASTJP010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418021"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1046)
                     /locus_tag="VFQ23_17065"
     CDS             complement(87..1046)
                     /locus_tag="VFQ23_17065"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET9908362.1"
                     /translation="MSEKREPTRADLVRIRRERENTQQMKRAAIEAARAVLPVAARAE
                     TAKPRRKSANSGRGRRRFQVALPVSRTDFRPTAMPRFRFGWRSLSLIFAASLGVLIYL
                     AFNRPELRVVEAKITGNQLIAPSELNSVLNVAGQPIFVLIPEDLETRLRLHYPELVSV
                     DVEVSMPNNVSVNIVERQPVIRWEQAGGYTWISEDGIAFRPRGEVLGLISVIALSGPT
                     IETMSLDPLTPAPFISTEMVQSLKGLAGHVPPGESILFDSVLGFGWNDPRGWRVHFGT
                     SAADVELKMRVYESMVTSLTQRGIRPAMINVTYPTAPYYRMSQ"
     gene            complement(1043..2155)
                     /locus_tag="VFQ23_17070"
     CDS             complement(1043..2155)
                     /locus_tag="VFQ23_17070"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET9908363.1"
                     /translation="MEKKLHVAVIFGGRSGEHEVSLMSARSVLSVLDVQRYEVTQIGI
                     THEGVWLTGENVLEKFESNTTDGLLPVIVSPDPSEKGIFVLENGSGFRKWADVDVFFP
                     VLHGTFGEDGTIQGLFELADVAYVGAGVVGSAVGMDKGIFKEVMRANDIPVVESVMVL
                     RSEIEKDMGAVIEKAEKMGAYPLFAKPANLGSSVGVTKCRNRSDMQEGLMEAMSFDRR
                     VLIERGVGDVREIEVSVLGNDEPVASVCGEVIPSREFYSYESKYIDGTSGLQIPAELP
                     AETAEQIRQYAVRAYKLIDSAGMARVDFFVERDTNRIYLNELNTIPGFTKISMYPKLW
                     QASGLPYHKLVDRLIELALERKAERDHTSHTYRSQS"
     gene            complement(2163..3080)
                     /gene="murB"
                     /locus_tag="VFQ23_17075"
     CDS             complement(2163..3080)
                     /gene="murB"
                     /locus_tag="VFQ23_17075"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET9908364.1"
                     /translation="MIVPVRPIDHLYAKLGDKVKENVLLAPYTSARIGGPADIFITAD
                     SAAELARIVKLLWKQEMPYTILGGGSNVLISDKGIRGVVVLNRAKAVRFHSGDEPSVT
                     VESGVVFSNLANRCASKGFGGIEWAATVPGTIGGAVYGNAGAFGGDMAGNLMCADLLT
                     AEGRQQYSVEQMGYGYRTSVLKRGEMKAVVLLAQLSLKNSTKEEATVNIQQFGAHRKA
                     TQPPGASMGSMFKNPNGDYAGRLIETAGLKGTRIGNAEVSQLHGNFFINHGATKAEDI
                     RALVELVQKTVKEKFDAELELEIEFLGEW"
     gene            complement(3077..3331)
                     /locus_tag="VFQ23_17080"
     CDS             complement(3077..3331)
                     /locus_tag="VFQ23_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908365.1"
                     /translation="MIDQKKRTYQSETLSLLTLKLILTEAKRESPPQTRKEEQSDEEL
                     ALNLIASMKKSYKGLVSHPAALKAERASKVSMKKVAVLEP"
     gene            complement(3338..4732)
                     /gene="murC"
                     /locus_tag="VFQ23_17085"
     CDS             complement(3338..4732)
                     /gene="murC"
                     /locus_tag="VFQ23_17085"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET9908366.1"
                     /translation="MTHVHFIGIGGSGLSAIARLLLESGYTVSGSDRALSPFADEIRK
                     AGAKVYVGHHPRNVGGADWVVRSSAVTDDNPEVQAANLTGIPVYKRADFLGQLMTDKT
                     GIAIAGTHGKTTTTAMTAWVLHELKREPSFIVGSVMNNLGVNARAGKGKLFVIEADEY
                     DNMFLGLKPQIAVVTSVEHDHPDFFPTPEAMYSAFEKFTDLLPADGTLIACVEDAGAA
                     SLISHVRKQGKNIISYSVQGEMTINSPNWVQASRLKPNYRGGFDFIALSNLGDKGNES
                     IAVSLQVPGEHNVRNALAVLAIVDLLGLSSEKAVKALGEFTGTARRFQLRGEVNGISI
                     IDDYAHHPTEIKATLAGARARYPERRIWAVWQPHTFSRTQTLFLEFARAFKDADEVIV
                     TEVYAARELQQDFTSAEIVSSMPHLSARYIKTLPEVTKYLFENLQPGDVVLVLSAGDA
                     DQICTDLLNLFSER"
     gene            complement(4733..5263)
                     /locus_tag="VFQ23_17090"
     CDS             complement(4733..5263)
                     /locus_tag="VFQ23_17090"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET9908367.1"
                     /translation="MMSVVYLFWMYVILFALVGWMRGWAKELLVSFSVILALALNHVI
                     RRYIPIARDLAETDASLFWVRTIVLLVLVYFGYQTVISIPHLASRARSERLQDTLFGA
                     FLGGINGYLVAGSILYYMHVADYPFQNVISKPADPTLVQTVGQMMLYMPPQLLGEPGI
                     YFAIILAFVFVLVVYI"
     gene            complement(5269..6312)
                     /locus_tag="VFQ23_17095"
     CDS             complement(5269..6312)
                     /locus_tag="VFQ23_17095"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET9908368.1"
                     /translation="MYPALAVHSALAAKIPNMDALWVGSESGMEESLVKRQGLSFRSI
                     PAAGVHGVGLATLPRNLTMLGRGVLAARRILNDFEPDVMFFTGGYVAVPVALAGRSIP
                     ILLYVPDIEPGMALKSLSRFADAITVTTDQSQSYFNKPVYEVGYPLRSDLALWDRTTA
                     NKHLGISGEFPVLLVFGGSLGAHSINMSVLKILSELLEKFEIIHLSGEQDWQLLKDER
                     EILPAELAARYHVLPYLHEMGAALASADLVVSRAGASCLGEYPLFGLPAILVPYPHAW
                     RYQKVNADFLTRRGAAVTLEDHRLNDDLLVTLNVLMENPNKLKAMRAAMFELSHPRAA
                     EKIASALIELAGE"
     gene            complement(6272..7528)
                     /locus_tag="VFQ23_17100"
     CDS             complement(6272..7528)
                     /locus_tag="VFQ23_17100"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET9908369.1"
                     /translation="MGTRTFVNNRSGFWLFGGGGKRARGTDMLLVLTVVALVVFGLIM
                     LYSASFDFSFNEYGSSSYMFLRQLRWLGLGVILTVLLSYFDYHHWRRLVVFAMLGIIG
                     LLLTVLFINEIRLGASRTLYDGSYQPSEIAKLVTVIYLSVWLYAKRQYLHDLSLGLVP
                     LGVILGIVGGLIYLQPDLSAVGTVMILGGLLFFLAGADIKQIVFLLIAAIFMAWVVVQ
                     FSATGQDRVSSFVAGLQDPTNASYHVQRSFEAVVKGGVFGVGLGQADTKLTGLPFAPT
                     DSIFAVVAEELGLFGSVLLMTLYAALIWRGLVIARRAPDMLGTLLASGMTFWIGIEAL
                     INMAVMVGLMPFAGNALPFISAGGSNLISTMCAIGIMLNVSRQSGESAKAEKNEWRSF
                     GAVIDMRRGNGRRSVSRPRRSQRTRS"
     gene            complement(7507..>8163)
                     /locus_tag="VFQ23_17105"
     CDS             complement(7507..>8163)
                     /locus_tag="VFQ23_17105"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9908370.1"
                     /translation="SSHALDKALNGTYLQDGWLNLRDGNLLLPLISRGHISLRGDHNV
                     SNVLAAFAIGHAAGFAPDPMMQAVKDFHGVPHRLEFVRELRGVCWYNDSIATAPERSM
                     AAIRAFDEPIVLLLGGRDKDLPWEELMELVGERVDHVVLFGEAAEKIQQTIKNLGQSE
                     KPFTVTRADHLHEAVLKAAEVAETGDVVLLAPGGTSFDEFKDFAERGEFFRTWVQELS
                     "
BASE COUNT         2114 a         2275 c         1911 g         1863 t
ORIGIN      
        1 tagagttgtt atcttccact cgtccgacga gcgtacagac tttggttgtg ccgacgtcga
       61 tgcctacaac aatttcttcc ataatcttat tgactcatcc tgtaatacgg cgccgtcgga
      121 tacgtcacgt tgatcatcgc cggccggatg cctctctgtg ttaatgatgt gaccatcgat
      181 tcatagacgc gcatttttaa ctctacatcc gctgcgctgg taccaaagtg gactcgccag
      241 ccacgcgggt cattccagcc aaagcccagg actgaatcaa acaaaatgga ttcgcccgga
      301 ggcacatgac cggcaagccc tttcagcgac tgaaccattt ctgttgaaat gaaaggcgct
      361 ggagtgagtg ggtcgagtga catcgtttca attgtgggac cagacaatgc gatcaccgaa
      421 attagaccca gcacttcacc tctcggacga aaagcgattc catcctctga gatccacgtg
      481 tacccacccg cctgttccca gcggatgact ggttgtctct cgacgatatt caccgagacg
      541 ttattcggca tggaaacttc cacgtccaca gaaacaagct cggggtagtg aagccgcagt
      601 cgtgtctcca aatcttcagg gataaggaca aagataggtt ggccggcaac gtttaacaca
      661 gagttaagtt ccgagggggc aatcagttgg ttgccggtga tttttgcttc caccacacgc
      721 aactcaggtc ggttgaaggc gaggtagatg agcacaccaa gcgaagcagc aaatatcaaa
      781 gacagcgagc gccacccaaa acgaaaacgt ggcatcgcgg tcgggcgaaa gtcggtacgt
      841 gatacaggca gggcgacctg aaagcgacga cgcccacgcc ctgaattcgc cgattttctg
      901 cgaggttttg ctgtctcagc ccgcgccgcg actggcagaa cagcgcgtgc ggcttcgatc
      961 gcagcgcgct tcatttgttg tgtattctcc ctctcccgcc gtatgcgcac gaggtcggcg
     1021 cgggtaggtt ctctcttctc actcatgatt gactccgata tgtgtgagac gtgtggtcgc
     1081 gttctgcttt gcgttccagt gccagctcga tcaagcgatc gaccagtttg tgatagggca
     1141 atccactcgc ctgccaaagt ttgggataca tactgatttt ggtgaaaccc ggtatggtgt
     1201 tcaattcatt caaatagatc cggtttgtat cacgttcaac aaaaaaatcc acgcgcgcca
     1261 tacctgcact atcgatcaac ttgtaggcac gcaccgcata ttggcgtatt tgttcagcgg
     1321 tttccgcagg tagttctgcg gggatttgta aacctgatgt accatcaatg tatttcgatt
     1381 catacgaata aaattcgcgg ctgggaatga cttcgccaca aacagaggca accggttcat
     1441 catttcccaa tacactcact tcgatctcac gcacatcccc tacaccgcgt tcgatcagca
     1501 cccggcggtc aaaggacatg gcctccatca agccttcctg catatcggaa cggttgcggc
     1561 atttcgtcac accaaccgaa gaacccagat tcgctggctt ggcaaagagc ggatacgcac
     1621 ccatcttttc agctttttca atgaccgcgc ccatatcctt ttcgatctct gaacgtaaca
     1681 ccatcaccga ctccacaacc gggatatcgt tggcgcgcat cacttccttg aagatacctt
     1741 tgtccatacc gactgcagac ccgacgacac ccgcgcctac ataggcgaca tcagccagct
     1801 caaacaaacc ttgaattgtt ccatcttcgc cgaaggttcc atgcaacacc gggaagaaga
     1861 cgtccacatc tgcccacttg cgaaaacctg aaccattttc aagaacaaag atgccttttt
     1921 cggaaggatc aggtgaaaca atcacgggta gtaatccatc cgtggtattg ctttcaaact
     1981 tttcgagcac attctcacca gtcaaccata cgccttcgtg tgtgatcccg atctgagtca
     2041 cttcgtatct ctggacatcc aatacagaaa gcactgaacg cgcagacatc agtgagacct
     2101 cgtgctcacc ggaacgtccg ccgaatatga ctgctacatg aagtttcttt tccatcaatg
     2161 atttaccatt cacccaggaa ctcgatttca agttccagct cagcatcaaa tttttctttc
     2221 acggtctttt ggacaagttc aaccaaagcg cggatatctt ctgctttcgt ggctccgtga
     2281 ttgataaaga agttaccgtg taattggctg acctccgcat tgccaatgcg tgtgcctttt
     2341 aagccggcag tttcgatcaa ccgcccggca taatcaccat tcggattttt gaacatcgag
     2401 cccatacttg cacccggagg ctgggtggct ttgcggtgtg caccaaactg ctgtatattt
     2461 acggtcgcct cctccttcgt ggaatttttc agactcaact gagccagaag gacgacagct
     2521 ttcatctcac cgcgtttcaa cacactcgtg cgatacccat atcccatttg ttcaacagaa
     2581 tattgctgac gaccctcagc tgtgagcaaa tctgcgcaca tcagatttcc agccatatca
     2641 ccgccaaacg ctccggcatt tccgtaaact gcaccaccaa tcgtgcccgg aaccgtcgcc
     2701 gcccattcga tgcctccaaa acctttggac gcacaacggt tggcaagatt gctaaagacg
     2761 actcctgatt cgacggtcac tgatggctcg tcaccactat gaaatcggac ggcctttgca
     2821 cgattcaata ccaccacgcc tcggatccct ttatcactga tcaacacatt cgacccaccg
     2881 cctaaaatgg tgtaaggcat ttcctgtttc cacaacagtt ttacaatcct cgcaagttct
     2941 gcagccgaat ctgctgtgat aaaaatatca gcaggtcctc caatgcgcgc cgaagtataa
     3001 ggagccagga gaacattctc cttcacctta tcgccaagtt tggcatataa atggtcaatc
     3061 ggtcgtacag gtacaatcat ggttccagca cagctacttt cttcattgag accttcgatg
     3121 cacgttcggc tttcaaagcg gcaggatggg acacaaggcc tttatagctt tttttcatac
     3181 tcgcaatcag gtttagggcg agttcttcat cactttgctc ttccttccgt gtttgcggag
     3241 gcgactccct tttcgcttcg gtcaaaatca acttcaaggt cagcaaagac agagtctcgg
     3301 actgatatgt gcgttttttc tgatcaatca tattcatcta cctttcgcta aaaaggttca
     3361 gcaaatcggt acagatctga tctgcatcac ctgctgaaag caccagcact acatcacctg
     3421 gctgaagatt ctcgaaaaga tatttcgtta cttcgggtaa cgtctttatg taacgggcag
     3481 aaagatgcgg catgctgctg acgatctctg cagaggtgaa atcctgttgc agctcgcggg
     3541 cggcatacac ctcggtgacg ataacttcat ctgcatcttt aaaggcacgc gcgaattcca
     3601 gaaaaagcgt ttgtgtgcgc gaaaacgtgt gtggctgcca tacagcccaa atgcggcgtt
     3661 caggataacg ggcacgtgcg ccagccagag tcgccttgat ctcggtggga tgatgagcat
     3721 aatcatcaat aatgctgatg ccattcacct caccgcgcag ttggaagcgc cgcgcggtcc
     3781 ctgtgaactc accgagcgcc ttcacagctt tttctgacga gagacccagc aggtcaacaa
     3841 ttgccagaac agccagcgca ttgcgcacgt tatgctcacc gggcacttgc aacgagacgg
     3901 ctatggattc gttccctttg tcacccaggt ttgataacgc aataaaatcg aacccgccgc
     3961 gatagttggg cttgagcctg cttgcctgca cccagttcgg actgttgatg gtcatctcac
     4021 cttgcacgct ataggaaata atattctttc cctgttttct cacatgtgag atcagggatg
     4081 ccgcgcccgc atcctccaca caggcaatca acgtcccatc agcagggaga agatccgtaa
     4141 acttttcaaa cgcggaatac atcgcttcgg gcgtggggaa gaaatccggg tgatcgtgtt
     4201 cgacgcttgt cacaacagcg atctgtggct taagccccag gaacatgttg tcgtattcat
     4261 cagcctcgat cacgaacaat ttgcctttac ccgcacgtgc attgacaccc agattattca
     4321 tcacactgcc aacaataaat gatggttcgc gtttaagttc atgcaatacc caggcggtca
     4381 ttgcggtggt cgttgtcttt ccgtgggtac ccgcaatcgc gatgccggtt ttatcggtca
     4441 tcaactgccc cagaaaatcc gcacgtttat agactgggat tccggtcaaa ttcgcggctt
     4501 gaacctcagg attgtcgtct gtaactgcag aggatctcac tacccaatct gcaccgccta
     4561 cattacgcgg atgatgccca acatacacct ttgcaccagc cttgcgaatt tcatcagcaa
     4621 acggggatag cgcgcggtca gagccgctca ccgtgtatcc gctctccagc aggaggcggg
     4681 cgatggcgga aagaccgctt cccccgatgc cgataaaatg gacgtgagtc atttagatat
     4741 atacaaccag tacaaataca aatgccagga taatggcgaa ataaatgcca ggttcgccga
     4801 gcaattgggg aggcatatac aacatcatct gtccgaccgt ttgaaccagg gtggggtcgg
     4861 caggcttgct aattacattc tggaacggat aatcagcaac atgcatgtag tacaaaatgc
     4921 tgccggccac caaatagccg ttaattcctc caaggaaagc gccgaagagt gtgtcttgta
     4981 aacgctcact acgggcgcgg gaggccaggt gtggaatgct gatcacggtc tgatatccga
     5041 agtaaaccaa caccagcaga acgatggttc gaacccagaa aagggatgca tctgtctcag
     5101 cgaggtcgcg cgcgattggg atgtatcgcc gtatcacgtg gttcagcgca agcgcaagaa
     5161 tgacgctaaa cgaaacaagc aattcctttg cccagccgcg catccagcca accagggcga
     5221 agagaataac gtacatccag aagagataaa caacgctcat cataggcctt attctcctgc
     5281 cagctcgatc agcgcactgg cgatcttctc agccgcccgc ggatgtgata attcgaacat
     5341 cgcggcgcgc attgctttca atttgttcgg gttttccatc agaacattca aagtcacaag
     5401 caagtcgtcg ttgagacgat gatcttccag cgtaacagcc gcgccgcgcc gagtcagaaa
     5461 atctgcattc actttctgat agcgccatgc atgtggatag ggcacgagaa tcgcaggcag
     5521 gccaaacaac ggatattcac ctaagcagga ggcgcctgca cgggatacaa ccagatccgc
     5581 tgaagccagg gcggcaccca tttcatgcaa atagggcaaa acatgataac gagcagcaag
     5641 ttccgctggc aggatttcac gctcatcttt gaggagctgc caatcctgtt caccagagag
     5701 atgaatgatt tcgaatttct ccagcagttc actcaggatc ttcaataccg acatattgat
     5761 tgaatgagcg ccaaggctcc caccaaatac caaaaggaca ggaaattctc ccgagatacc
     5821 caggtgtttg ttcgcggttg tccgatccca gagcgcaagg tcagagcgga gtgggtatcc
     5881 aacttcatat acgggcttat taaagtacga ttgtgattga tctgtggtta ccgtgatcgc
     5941 gtccgcaaat ctggaaagcg atttaagtgc catcccgggt tcaatgtctg gcacataaag
     6001 aagtatggga atagatcggc ccgccaacgc tacaggtaca gccacatagc ctccggtaaa
     6061 gaacatcaca tctggttcaa agtcattcaa gatgcgtcgt gcagccagga cgccgcgccc
     6121 aagcatggtc aggttacgtg gcagagtcgc cagaccaacg ccatgcacac ccgcggcagg
     6181 gatgctgcga aacgaaagac cctggcgttt aaccagtgac tcttccatcc cgctttcgct
     6241 gcccacccac agcgcatcca tgttcgggat cttagctgcg agtgcgctgt gaacggcgag
     6301 ggcgggatac actccgcctc ccgttccccc ggcgcatatc aataaccgca ccgaatgacc
     6361 tccattcgtt cttttctgct tttgcactct cgccactctg acgagagacg ttcaacatga
     6421 taccgatcgc gcacatcgtt gaaatcaaat tggagccgcc ggcactgatg aagggcaagg
     6481 cgttcccggc gaagggcatg agccccacca taaccgccat gttgatcagt gcctcaatgc
     6541 caatccagaa cgtcatacct gatgcaagaa gagtgcccag catatctggt gcgcggcggg
     6601 caatgacaag tccgcgccag atgagtgccg catataaagt catcagcaat acggatccaa
     6661 acaaaccaag ttcctctgca accacggcaa agatgctatc ggtgggcgca aaagggagtc
     6721 ctgttaattt tgtatctgcc tgccccaacc caacgccaaa cacaccacct ttcactaccg
     6781 cttcaaacga tcgctgcaca tgataggatg cattggtggg gtcctgtaaa ccggctacaa
     6841 atgaactcac acgatcctgc ccggttgcac tgaactgaac cacgacccag gccatgaaaa
     6901 tcgctgcgat cagcaaaaat acaatctgct tgatatcagc tccggctaag aagaataaca
     6961 gccctcccag gatcatcact gtacccacgg cactgaggtc aggttgaaga tagatcaatc
     7021 ctccaacaat acccaggatt acgcccagcg gcaccaaccc aagactgagg tcatgcagat
     7081 attgtcgctt ggcatacaac cagacggaga gatagatcac ggtgacaagt tttgcaattt
     7141 cagaaggctg gtaagaacca tcatacagcg tgcgtgaagc acccaatcgg atttcattga
     7201 taaatagaac tgtgagcagc aagccgatga ttccaagcat agcgaacaca accagcctgc
     7261 gccagtggtg ataatcaaaa tacgacaaca aaactgtaag tatgacgccc agtccaagcc
     7321 atcttaattg gcggaggaac atataggatg aagatccata ttcattgaag gagaaatcga
     7381 aggacgcaga gtacagcatg atcaaaccaa acaccaccag tgcgaccacc gtcaacacaa
     7441 gaagcatatc ggtgcctcgt gcacgtttgc cgccgccccc aaacagccaa aagccggatc
     7501 gattatttac gaaagttctt gtacccatgt tctaaaaaat tctccgcgtt ctgcaaaatc
     7561 cttgaactca tcaaaacttg tgccgcctgg tgctagcaga acaacatcac cagtttcggc
     7621 aacttccgcg gctttcagga cggcttcgtg caaatgatcc gcgcgtgtaa cggtaaatgg
     7681 cttctcactt tgcccaagat tttttatcgt ttgctgaatt ttctctgccg cttcaccaaa
     7741 caacacaaca tgatcaacgc gttcgccgac caattccatg agttcttccc acggaagatc
     7801 cttgtcacgg ccgcccagca gcaggacgat cggttcatca aatgcacgga tggctgccat
     7861 gctccgctcg ggcgcggtcg caatcgaatc gttgtaccag cagacaccgc gcagttcccg
     7921 aacaaactcc aggcgatgag gcacaccatg aaagtctttc accgcctgca tcatagggtc
     7981 gggagcgaag ccggcggcat gaccaattgc aaacgcggcc agcacattcg aaacgttatg
     8041 gtcaccgcgt aatgaaatat gcccgcggga gatcaaaggc aggagcaagt tcccatcgcg
     8101 caggttgagc catccatcct gcagataggt tccattcaag gctttatcca gtgcatgcga
     8161 act
//
LOCUS       DASTJP010000583         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_315029, whole genome shotgun sequence.
ACCESSION   DASTJP010000583 DASTJP010000000
VERSION     DASTJP010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315029"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1728
                     /locus_tag="VFQ23_17110"
     CDS             367..1728
                     /locus_tag="VFQ23_17110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9908371.1"
                     /translation="MKTFQVTLVNSMVAFVVNNFVWFAVTFWVYLETKSVIATSVMAG
                     IFTLTVAFSGFLLGSLVDRYPKKQVMMLSSIVSLVLYAVAGAIFITTPYEAFADASSI
                     PLWIFIVLALVGAIIGNLRSIALSTLVTILVHEDERDKANGLVGTANGIAFLGASIFS
                     GLAVGYLGMFWVMVIAIVASLLVIAHLATLTIPDLKKKIRHSHDGELEHEHAEPHEGI
                     DVPGTIKAIQLVPGLFGLIFFHTFNNFLGGIFMSLMDAYGLLLVSVQVWGTLWGVLSL
                     GFIVGGMIVARRGLGKNPLRTLFLANIVMWSICCVFTLKASIVLLGVGMFVWLCLIPA
                     VEAAEQTILQKVVPPERQGRVFGFAQSVEQAASPITAFLIGPIAQLIFIPYMTTGTGV
                     DSIGSWFGAGADRGLALLFTIAGLIGLAVTLLAMRSYSYRALSERYKDEDQGPDLAEA
                     YTA"
     gene            1896..3290
                     /locus_tag="VFQ23_17115"
     CDS             1896..3290
                     /locus_tag="VFQ23_17115"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9908372.1"
                     /translation="MTITSNSGPISIPELRKTIAGRIITPDDELYDKARTVFYGGVDR
                     HPAVIVRVKNTEDVVRVISLAHDSGLELAVRSGGHSVVGYSVTEGGIVLDLSDMRDMQ
                     IDVNEKTAWAEAGITAGEYTAAAGAHGLATGFGDTGSVGLGGLTLGGGVGYLVRKYGL
                     TIDDLLAAEVVTANGEVLHVDANSRPDLFWAVRGGGGNFGVVTRFQFRLHEVGDIYGG
                     MLFLPATPDVMAGFIAEAESAPEELSTIANIMNAPPMPFLPEQYHGQLIIMAMLVFAG
                     DAESGERALAPFRALATPIVDMVRPMKYPEIYPPEEGEYHPVAASRTMFVDTIDRPVA
                     ELILDRLSASNAMMAVAQLRVLGGAMARVPVEATAFAHRNSRIMVNLAALYQSPEDKS
                     IHETWVSDFAAALQQSDKGAYVNFLGEEASDGIHAAYPAPTWERLRTIKLQYDPDNLF
                     RLNQNILPAERVGE"
     gene            3365..4180
                     /locus_tag="VFQ23_17120"
     CDS             3365..4180
                     /locus_tag="VFQ23_17120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9908373.1"
                     /translation="MAKTKFKKGYASVNGLEMYYEIHGTGQPLVLLHGAFSAIGTSFG
                     KILPELSKTRQVIGFELQAHGHTADIDRPLSPEGMADDVAAAMKQLGLEQADVFGYSM
                     GAFVALHLVLRLPQLVRKLVLASVSYTMSGVHPGLMEGLGEMAPDMMYGSPWHNEYLK
                     IAPNPEHFARFFAKKTEMDRHTRDLSADDIKGIKSPTLIIIGDSDLIRPEHAVEMFRL
                     LGGGIFGDTPAGLPNSQLAILPGTSHVSIVGRAELLVPMIASFLDAPMPKDGP"
BASE COUNT         1016 a         1011 c         1138 g         1127 t
ORIGIN      
        1 accgaacact ttgacaaaaa acagagggct tactatttga atctgaagaa actgggctaa
       61 cgacattagt ttagacaaac gagcttcaca aatcgaagaa acgcatactt tttttgattg
      121 aaagccctgt aacagtggcg gatgtttctt cgtttgaaag atagatggtc cgtcctgtgg
      181 agcctgctat tgtttcatct tgacgaaaaa gaacatacgt tctacaatta gaaaattctt
      241 atgggtgggc agcatcattg acttgctcac ccataaattt tcggttgtaa ttggatgcgg
      301 caatacagag ggtgaaagtc gaaacaatca aattgtattg aatggagagc taacttgaac
      361 gagaaaatga aaacatttca agtgactttg gtaaattcaa tggtcgcttt cgttgtcaac
      421 aattttgtct ggtttgctgt cacattttgg gtatacctcg aaacaaaatc tgtcatcgcc
      481 acttcggtaa tggcgggcat cttcaccttg acggttgctt tctcaggatt cctgcttgga
      541 tccctggttg accgctatcc gaagaaacag gtgatgatgc tttcgagtat tgtttctctt
      601 gttttatatg cagttgccgg tgcgattttt atcactacgc cctatgaagc attcgcagat
      661 gcatcaagca taccgctttg gatcttcatt gtgcttgcgc tcgttggcgc cattatcggt
      721 aatctccgca gtattgccct gtcaaccctt gtcactatcc tagttcacga agacgagcgg
      781 gataaagcca acggacttgt tggcactgcg aatggaatcg catttcttgg tgcatccatc
      841 ttcagtggat tggcggtggg gtatctcggc atgttttggg tgatggtgat tgcgattgtg
      901 gcaagcctgc tggtgattgc tcaccttgcc accctgacca tacctgattt gaagaaaaaa
      961 atacgccatt cacacgatgg ggaactggag catgaacatg ctgagccgca cgaaggtatt
     1021 gatgttccag gaacgatcaa ggccattcag cttgttcctg ggttgtttgg tctgatcttt
     1081 ttccacacct ttaataactt cctgggtggc atctttatgt ctctcatgga cgcatacggg
     1141 ttattgctgg tgtcagtaca ggtctggggc acgttatggg gcgtgctaag cctgggcttt
     1201 atcgtcggtg ggatgattgt tgcaagaaga gggctgggga aaaaccctct gcgaacgctc
     1261 tttctggcga acatcgtcat gtggagtatt tgttgtgtct ttacgctcaa ggcatccatt
     1321 gtattgctgg gagtcggaat gttcgtttgg ttgtgcctga tcccggcagt ggaagccgca
     1381 gaacagacca tcctgcaaaa ggtggttccg ccagagcgtc aggggcgtgt gttcgggttt
     1441 gcccaaagcg tggagcaggc tgcgtcgccg attacggcgt ttctgatcgg tccaatcgcg
     1501 caattgatct ttattccgta catgacgacc ggtaccggcg tcgactcgat cggctcatgg
     1561 tttggggcag gggccgaccg tgggctggct ctcctgttca ccatcgccgg tttgatcggt
     1621 ctggctgtta ccttgctggc gatgaggtct tattcctacc gcgccttgtc ggaacgatac
     1681 aaggatgagg atcaaggacc tgacctggca gaagcctaca ctgcatgaca aaccacgatg
     1741 agactgaaat tagccacaat taactcgact tcatatgctt atgtaaacca ttaattttta
     1801 gttagtcaat tcaaatgcac ttttagtatc caaacgaaaa ttctcttctg attcaatagt
     1861 atattcatag agctttgaaa ggagtctcac tacccatgac aattacttca aactccggcc
     1921 cgatttcgat tcctgaactg cgcaaaacta ttgccggacg gataattacc cccgacgatg
     1981 aattatatga caaggctcga actgttttct acggtggagt ggatcgacac ccggccgtga
     2041 ttgttcgagt taaaaacact gaggatgtcg tccgcgtgat ttcgctggcg catgattctg
     2101 gtttggaatt ggctgtccgt agtggcggcc atagcgtcgt tggttatagc gtgaccgaag
     2161 gcggcatcgt tctggatcta tccgacatgc gtgatatgca gatcgatgtg aacgaaaaaa
     2221 cagcctgggc cgaggctggc atcaccgcag gagaatacac ggcagcggcg ggtgcgcatg
     2281 gacttgcaac gggctttgga gatactggct cggtgggtct tggaggacta accctgggag
     2341 gtggtgttgg ctatcttgtg cgaaagtatg ggctaaccat tgatgatctc ttagcggccg
     2401 aagtcgtcac agcgaatggc gaagtccttc acgtagatgc taactctcgc cccgatctat
     2461 tctgggccgt tcgcggcgga ggcggaaact ttggagtagt tactcgcttc cagtttcgcc
     2521 ttcacgaagt tggtgacatt tatggcggca tgctgttcct acctgcaacg ccagacgtga
     2581 tggcagggtt cattgccgaa gcagaatctg ctcccgaaga gctttcgaca atcgccaaca
     2641 ttatgaatgc tccgccaatg cccttcctgc cggagcagta tcatggacaa ttgatcatta
     2701 tggccatgtt ggtgtttgct ggcgatgccg agtcgggcga acgagcgctt gcaccatttc
     2761 gcgcactagc cacaccaatt gtggatatgg tgcggccgat gaaataccct gaaatctatc
     2821 ctcccgagga aggtgaatat caccctgtgg cagcctcgcg caccatgttt gtcgatacga
     2881 tcgatcgccc ggtagcggaa ctgatccttg atcgactttc agcttcgaat gcgatgatgg
     2941 cagtcgctca attgcgggta ctgggtggcg caatggctcg cgtgcctgtc gaggctaccg
     3001 catttgccca ccgcaactcg cggattatgg tcaatcttgc tgcgctgtac caaagccctg
     3061 aagacaaatc cattcacgaa acctgggtct ctgattttgc cgccgcgctt caacagtcag
     3121 acaagggtgc gtatgttaat ttcctgggcg aggaagcgag tgacggcatt catgcggcgt
     3181 atcccgcgcc gacatgggag cggctgagaa ccatcaagct tcaatacgat ccggataatc
     3241 tcttccgcct caaccagaat atccttcccg cggagcgcgt gggagaataa aaacggtgtt
     3301 attcaagaat gcattaacta attgatttgg ggattgtcaa agtccccaag tgacaggaga
     3361 tttaatggcg aaaactaagt tcaaaaaggg ctatgcttct gtgaatggac tggagatgta
     3421 ttatgagatt cacggtaccg gtcaaccgtt agttctgcta catggtgcct tttctgcgat
     3481 cgggacgtcg tttggaaaaa tcctgcccga gctttcaaaa acgcgacagg tgatcgggtt
     3541 tgagttgcag gcgcatgggc atacggcgga cattgaccgt cctttgtccc ctgaaggtat
     3601 ggcagatgac gttgccgcgg caatgaagca acttgggttg gaacaggcgg acgtgtttgg
     3661 gtacagcatg ggggcgtttg tcgcgcttca tcttgtcctc cgtcttccac agttggtacg
     3721 caaactggtg ttggcatcgg tcagttacac aatgagcggc gtgcatccgg ggcttatgga
     3781 aggcttagga gagatggcgc ccgacatgat gtacggatcg ccatggcata atgaatatct
     3841 aaagattgcc ccaaacccag aacactttgc caggtttttc gcgaaaaaaa cagagatgga
     3901 ccgccatacc cgggacttat cggcagatga tattaaagga attaagtctc cgactttgat
     3961 cattattggc gattcggatc tcatccggcc cgagcatgcc gttgaaatgt ttcggctcct
     4021 tgggggaggc atttttggcg atacgcccgc cgggttacca aattcacaac tcgcaatcct
     4081 gccaggtact tcacatgtta gcatcgtagg tcgcgccgag ttacttgttc cgatgatcgc
     4141 atcctttctc gatgctccca tgccaaaaga tgggccatag atgaacatga aaaaaaccga
     4201 accagcggaa accgacctcc ctcgcagcat tggaaacccc gcccgcagag cgctggaaca
     4261 tgcagggtat acaaaactta aacagttgac ca
//
LOCUS       DASTJP010000584         4342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_161164, whole genome shotgun sequence.
ACCESSION   DASTJP010000584 DASTJP010000000
VERSION     DASTJP010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161164"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFQ23_17125"
     CDS             complement(<1..917)
                     /locus_tag="VFQ23_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908374.1"
                     /translation="MKFVFLSPHFPPNFYHFAVRLKQHGVTVLGLADENYDSLRPELK
                     GALTEYYRVNDMHNYDELVRALGYFTNHYGKLDRLDSHNEYWLETEAGLRTDFNIFGL
                     RLDDIQRVKRKSVMKEVFVNAGVKVARGRVIGNLNDALDLVAETGYPLVAKPDVGVGA
                     AATYKIHDQAELERFFVEKPPFDYLLEEYILGDIETFDGLTDFDGNLVFVNSLAYSAG
                     IMETVLADDHVYYYTRREIPADLEEVGRRVLREFGVRERFFHFEFFRRPETGELWALE
                     VNMRPPGGMSLDMFNYSCDIDIYDAWASLV"
     gene            1041..2276
                     /locus_tag="VFQ23_17130"
     CDS             1041..2276
                     /locus_tag="VFQ23_17130"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9908375.1"
                     /translation="MTLPNRPLLNANLRWFLAGMILANIAGQMLYSMLSLYMINLGAQ
                     VAEVGLVFTLASLVPMALQIFGGWLSDTIGRLRVMALGSSIAVFGYAIIALATSWEWV
                     LLGLSVEYVSNSVVGPSFGAYISEQSDESQRGRVFGVTSSIYMTVTVIGPALAGLLAN
                     RFGFKFMMSVALAFYFAATIVRVWMATSEHFAAKQDAEKPTWEGFRTQISAVFTLLLA
                     GGILTWIWITDAIGDTAANMIDQLTPIYLSDIGSLNVEQIGYINAVRGLAVIIVSPLA
                     GRLVDRSSERVSIVAGFILQAIGMSLFLFSETLVGFGAAMLFSGAAVGCLIPGYDSLI
                     SKVVPEERRGLAFGLFGTTLGILSLPMPWIGAQLWERYSPQTPYSFVFIACLISIIIV
                     WSKFRLPKQSEDAAVASSS"
     gene            2304..2819
                     /locus_tag="VFQ23_17135"
     CDS             2304..2819
                     /locus_tag="VFQ23_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908376.1"
                     /translation="MSKTIEVQTANLNFPAMCVICMSPSTKLYEVSQVYSRGTKSATV
                     QIHVPMCTSHFEAATSKSLVEKIIAILAIILGICSGLAALAILVTRWVGDDSLILKLI
                     IGSIVGFGVFVMAWALIALWVAPMFASTESKEARNAVRIVLVSPHAEVLRLEFQNEQL
                     AEIVQKQNSFS"
     gene            2906..4330
                     /locus_tag="VFQ23_17140"
     CDS             2906..4330
                     /locus_tag="VFQ23_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HET9908377.1"
                     /translation="MTYKISFGTDGWRGEIADDYTFENLRRAAQGFASYLLEQGKEGQ
                     WVVVGYDKRFHSENFAAAAAEVLAANGLKVYLTEGPTPTPTIAFAVVDKKACGAVNIT
                     ASHNPPTDNGFKVRDENGSAIDPEGLKRIEALIPDSAESIKRVKLNEALRDKNVVYFD
                     ASESYIANLKKLVDLQKIKDAGFTVLVDAMWGNGAGWFSQLLEGGKTNVIEIHNERNP
                     IYPEMKRPEPIRPNIDAGLTATVKNNADVLLITDGDADRCGIGDENGEFINQLRVYAL
                     LAFYLLEVRGERGDIVKTLSTTNMLNKLGEIYGVPVHETGVGFKYVAPKMLETNAMIG
                     GEESGGYAFRGNVAERDGILAGLYFLDFMVRTGKKPTELLQMLFEKLGNEYFYDRVDT
                     PFEGERKLRKKLILDAKPETIGGLKVTELVTVDGFQFRLEDGGWLLIRFSGTEPIMRV
                     YCETTHKDKVKAILDDGLKIAGIK"
BASE COUNT         1076 a         1075 c         1096 g         1095 t
ORIGIN      
        1 acaaggcttg cccaggcatc ataaatatcg atatcacacg aatagttaaa catgtccaaa
       61 ctcatcccac ccggtggacg catattaact tccagcgccc aaagctcccc agtctcaggt
      121 cgccgaaaga actcgaagtg aaaaaaccgt tcgcgcactc caaattctcg caaaacccgc
      181 ctccccacct cttccagatc agcaggaatc tcacgcctgg tatagtaata gacgtggtca
      241 tcggcaagaa ccgtttccat aatccctgca ctataagcca gtgaattgac aaatacaaga
      301 tttccgtcaa aatctgtcag cccatcgaac gtctcgatgt caccgagaat gtattcttca
      361 agcagataat caaagggagg tttttcgaca aagaaacgct ccagctctgc ttgatcatgg
      421 attttatacg tggcagctgc ccccacgccg acatccggct ttgccacgag gggatatccg
      481 gtttcggcga ccagatccag cgcgtcgttt aggttaccaa tcacgcgtcc gcgcgcgact
      541 ttgactccag cattgacgaa tacctctttc atcaccgatt ttcgcttcac acgttgaata
      601 tcatccagcc gcaagccaaa aatgttgaag tcagttcgca ggcctgcttc ggtttcgagc
      661 cagtattcgt tgtgcgagtc caggcggtca agttttccgt agtgattcgt aaaatatccc
      721 aatgcgcgca ccagctcatc gtaattgtgc atatcgttga cacggtaata ttcggtcaat
      781 gcgcccttaa gttccgggcg aagtgagtcg tagttttcgt cggccagccc taaaactgtg
      841 acgccgtgtt gcttgagtcg aacagcgaaa tgataaaagt ttggcggaaa atgaggggag
      901 agaaatacaa atttcatagc tcggcctttt gacttgccca actataccaa atttttacgg
      961 gcgctccgtt ccacagatcc gtttgggatt gcctgtataa ttcgagaatc cccgctgatg
     1021 cagcccaagt ttagataaca atgacacttc ccaacagacc tcttctcaat gcgaatttaa
     1081 ggtggtttct tgcaggaatg atcctcgcca atatcgcagg gcagatgctt tatagcatgc
     1141 tttctttgta catgatcaat ctgggcgcgc aagtggcgga ggtcggtctg gtattcacgc
     1201 tcgcttctct cgttcccatg gccttgcaga tatttggcgg ctggctctca gatacgatcg
     1261 gccgcctgcg agtgatggct ttgggaagtt cgatcgcggt cttcggctac gccatcatcg
     1321 ctttggccac cagctgggaa tgggtgttgc tggggcttag tgtcgaatat gtttcaaact
     1381 cggtcgttgg acccagtttc ggcgcctata tctcggaaca atctgacgaa agtcagcgcg
     1441 ggcgagtctt cggtgtgacc tccagcatct atatgacggt gaccgtgatc gggcccgcgt
     1501 tagctggtct gctggcaaat cgttttgggt ttaagttcat gatgagcgtg gcactcgcgt
     1561 tttatttcgc cgcgacgata gtaagggtat ggatggcaac gtctgaacat ttcgctgcga
     1621 aacaggacgc cgagaaaccc acctgggaag gcttcagaac acaaatcagc gcagtcttta
     1681 cgctgctgct cgcaggtgga attctcacat ggatctggat aacggatgcc atcggcgaca
     1741 ccgccgccaa catgatcgac cagctcacgc cgatctacct ctcggatatc ggatcgctca
     1801 acgtcgaaca aatcgggtac atcaacgccg tccgtggact tgccgttatt atcgtttcgc
     1861 ctctggcagg ccgactcgta gacagatcta gcgaacgagt cagcattgtg gctgggttta
     1921 tcctccaggc catcggcatg tcgctttttc tgttttcaga aaccctggtc gggtttggag
     1981 cagccatgct ctttagcggt gcagccgtgg ggtgcctcat ccccggctat gactcgctga
     2041 tctcaaaggt cgtgcctgag gagaggcgcg ggctggcttt tggacttttc ggcactacgc
     2101 ttggtatcct ttcgctgccg atgccctgga tcggcgcgca actatgggag cgatattctc
     2161 ctcaaacgcc ctatagcttt gtttttatag catgtttgat ctccatcatc atcgtctggt
     2221 cgaagtttag attgccaaag caaagtgaag acgcggcagt tgcaagttca agttgatgaa
     2281 attctggctg taaaggaaag gctatgtcca aaacaatcga ggtacagaca gcaaatctaa
     2341 atttcccggc gatgtgcgtc atttgcatgt cgccctcaac gaaactctat gaagtgagtc
     2401 aggtctattc tcgtgggaca aaatcagcga cggtacaaat ccatgtgccc atgtgtacgt
     2461 ctcactttga agccgcaacg tccaaaagcc tggtcgaaaa aataattgcc atattggcaa
     2521 tcattctggg aatttgttcg ggactcgccg cgctggcgat tctggtcaca cgctgggtag
     2581 gcgatgacag ccttattctc aagctcatca tcggcagcat tgtaggtttt ggagtgttcg
     2641 tcatggcctg ggcgctgatt gcgttatggg tcgccccaat gtttgccagc acagagtcga
     2701 aagaggcgcg caatgcggta cgaattgtgc ttgtctcgcc acatgcagag gtgttacgtc
     2761 tggagttcca aaacgagcaa ttggccgaga ttgtgcaaaa gcaaaattca ttttcctgag
     2821 acagatattt tgtcagccag tctttttctt tctcactccc actgatataa tttcgttgac
     2881 cctattccat ttcatggagt tttcaatgac atacaaaata agcttcggaa ctgacggctg
     2941 gcgcggggaa attgcagatg attacacatt cgaaaatctc aggcgggccg cccagggatt
     3001 cgcttcgtat ctccttgagc agggcaagga aggtcaatgg gtggttgtcg gatatgacaa
     3061 gcgtttccat tcagaaaatt tcgctgctgc tgccgccgaa gtgttggcag caaatggctt
     3121 gaaggtctat ctgactgaag gacccactcc cacccccacc attgcctttg ccgttgtcga
     3181 taagaaggct tgtggagcgg ttaacatcac tgcctcccac aaccccccta cagataacgg
     3241 gttcaaggtt cgagatgaga atggaagcgc cattgacccc gaaggtctga aacgaatcga
     3301 agcgctgatc ccggattcgg ctgaaagcat taaacgggta aaactcaatg aggcactgcg
     3361 cgataaaaac gtagtctatt ttgatgcctc tgagtcgtat atcgctaacc tgaaaaaatt
     3421 ggttgatttg caaaagatca aagatgcagg tttcactgtg ctggtggatg ccatgtgggg
     3481 taacggcgca ggctggtttt cgcagcttct tgaaggcggg aagaccaatg tcatcgaaat
     3541 ccacaatgaa cgcaatccta tctaccctga aatgaagcga ccggagccaa tccgtccaaa
     3601 tatcgatgcc ggtctcactg caaccgttaa gaacaatgca gacgttttat taataacaga
     3661 cggcgacgcc gaccgctgcg gcattggtga tgagaatggc gaattcatta atcagttgcg
     3721 agtgtatgcc ttgctcgcct tttacctgct cgaagttcgc ggcgaacgcg gagatatcgt
     3781 aaagactctt tccaccacaa acatgttaaa taaactcggt gagatctatg gcgttccagt
     3841 gcacgaaacc ggcgttggat tcaaatacgt tgccccgaag atgctcgaaa cgaatgccat
     3901 gattggtggc gaagaaagcg gcggctatgc gtttcgcggc aatgtcgctg agcgcgacgg
     3961 cattttggca ggactgtact tcctggactt tatggtacgc actggcaaga agccgaccga
     4021 attgctccag atgcttttcg aaaagttagg gaatgaatac ttttatgatc gggttgacac
     4081 cccatttgaa ggtgagcgca aattaagaaa aaaactgatt ctggatgcta agcccgaaac
     4141 tattggcgga ctgaaagtaa ccgagctcgt tacagtagat ggatttcagt tcaggctcga
     4201 ggacggcggc tggcttttga tcaggtttag cggaactgaa cccattatgc gagtatattg
     4261 cgaaacgacc cacaaagaca aggtaaaagc cattctggat gatggcttga aaatcgcagg
     4321 cattaaataa tgcattttct gt
//
LOCUS       DASTJP010000585        27935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1101100, whole genome shotgun sequence.
ACCESSION   DASTJP010000585 DASTJP010000000
VERSION     DASTJP010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 27935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27935
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101100"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VFQ23_17145"
     CDS             <1..1281
                     /locus_tag="VFQ23_17145"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET9908378.1"
                     /translation="FILDPAFDRSSTRRKNFLYEGLHALDKDLRSRGSYLVIRNGRPI
                     AVLQQLLTQTQASAIYAEEDFTPYAINRDEMIARHLPLELIQGQTVHHPSSVLKPDGK
                     PYTVYTPFSKTWKAKLPFLIELFPIPDQIKTPSGFSTEPLPAFKVNPLFPAGEQEALV
                     RLEEFLHKRIFSYSEDRNRMDLDGTSALSPYLHFGMLGLRQAVSAARQVMGGVLSLSK
                     DQKRSGAAETWLNELIWREFFIQILYHFPYVSQRSFNPSFTNIQWRNNEEEFKSWKTG
                     RTGVPIVDAGMRQLKEIGWMHNRARMIVASFLVKDLLIDWRWGEAWFMENLLDGDLAA
                     NNGGWQWTAGTGTDAAPYFRIFNPVLQSRKFDPQGDYIRKWIPELRDVDAKDIHAPWE
                     AGIKVKGYPEKPIIERDKERTLLAYKMSKEELRA"
     gene            1281..1826
                     /locus_tag="VFQ23_17150"
     CDS             1281..1826
                     /locus_tag="VFQ23_17150"
                     /inference="COORDINATES: protein motif:HMM:NF023096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HET9908379.1"
                     /translation="MENKRDLSVPMSRANVIVMIIVLPVVIIQFVGFIVLNGLDGLGI
                     TWGYPVLLIAIVLGVVLHELIHGLSWVIFGGKPFSAVKFGFQWKSLTPYAHLKEPVEV
                     NPYRIGGFMPWFVLGILPYFISLALGNGNLFWFSLIHTSAAGGDWLILWLIRNVKTGT
                     LVEDHPTNAGCYVIEPETATR"
     gene            1833..2348
                     /locus_tag="VFQ23_17155"
     CDS             1833..2348
                     /locus_tag="VFQ23_17155"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908380.1"
                     /translation="MKEAKVLIRSLVEIDIPEIARAFEIIGWNKPIAQYERYYREQAH
                     KARDVYVAFVDGQFAGYLTICWTSNYGPFREENIPEIVDFNVLPKFRMQGIGAQLMDK
                     AENEIAKVSSVAGIGVGMDPDYGAAQRLYVKRGYIPDGRGLYYRGHHPSGGEKITMGD
                     DLALYLTKKLK"
     gene            2352..3617
                     /locus_tag="VFQ23_17160"
     CDS             2352..3617
                     /locus_tag="VFQ23_17160"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9908381.1"
                     /translation="MTIRSIHPETGRAALRSLMKDRSPLGPLRVMAKHVGRFFQIPLP
                     MFKPFVVFGPEANRKVLVTERDKMLWRNTDPVTDLLGRGVLVVDGEEHDHYRKLMEPP
                     LHPSTFHSYTDIMIRQTDRVSSLWKEGEIVDMLVESRKIALLIIMQTLFSKDVWHDLP
                     KIWKPVLKSIEYISPGAWILWRKIPRPGFHKPLQALDEYLFGIIRERKNGNKEHDLLQ
                     HLIDAGLSDKVIRDQMLTMLIAGHDTSTALLAWIFALLGQHPEVYKQLDQEVNLMEKS
                     PLLDQVIKESLRLYPPIHIGNRRVAQELQLDEGNVPSGERLFYSIYLTHRDPAIWENA
                     DVFCPERFSHGKKTPPFSYVPFGGGPRVCIGAVFGQAEARIVLARLLQTYHFEFTNHK
                     IRAHMGATLEPRPGVFMKVTKRKVIKPQI"
     gene            3626..4468
                     /locus_tag="VFQ23_17165"
     CDS             3626..4468
                     /locus_tag="VFQ23_17165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HET9908382.1"
                     /translation="MTYFGFLLRFLCIPILIFLAITYWDNKNHRQTHGFRNGRGVWVA
                     IGVHILLAVVYTTPWDNYLVATGVWYYNPELVTGLLIGYVPIEEYTFFVLETVLSGLW
                     WWFLARRVSRLPEGKGLGVREGLWPNKLIYLSSGVLTLLWLIFTSLFFFGDSKWTYLS
                     ITLFWALPAILPQLLFGADILWHHRRLVLLGILIPGSYLSLMDIFALRETTWSISPSQ
                     TTGILFFGILPLEEVVFFFITNVLIIFGMTLLLSSVSQERFAEIKEQVRGWKKKRVVN
                     PQGR"
     gene            complement(4449..5816)
                     /locus_tag="VFQ23_17170"
     CDS             complement(4449..5816)
                     /locus_tag="VFQ23_17170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9908383.1"
                     /translation="MALSFFRDKDFFRAMLTIAVPVSFQQLITAGLNMIDVLMVGQLG
                     ETAVAALGLANQIFFLLILFLFGVTSGMSIFTAQFWGKGDTEQIHHILGICLIVAVSV
                     SLLFSLAATLIPEKLMMFYTEDPEVIRLGSDYLRIVGLSYVMMAISISYISLLRSITF
                     VKLTVVVSVLALVLKTCVAYLLIFGIGGLPALGVRGAAIGTSLGWAFECVLLVTLVYV
                     LKTPLAANFFTLFQFSRAFLGKVLTTSMPAAANEVLWSFGITTYNAVYARIGTDAIAA
                     VNINATIEELGFVLFIGLGNACSVMVGNKIGEGKKDLAFEYSRRFAILGVLSAIAGGF
                     IVFLLRERVIGLYDLSPTAVENLRWIMLVYSLSVWLRVFNFMLFIGALRAGGDTRYAM
                     FTELFSVWLVGVPSALLGGFVFHLPVYGVYAMVLIEEVVKAFIIVRRFLSRKWIHDLV
                     NVPAD"
     gene            complement(5840..6343)
                     /locus_tag="VFQ23_17175"
     CDS             complement(5840..6343)
                     /locus_tag="VFQ23_17175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908384.1"
                     /translation="MRHRFEYVLELKDLNPTTAPGTLAKATWRNPSLADQQILAELML
                     DSYRDTIDYDGETMDDSIREVESYFSSQHSAWLESSWLGFMENSLACASLVGFWKDRN
                     SPFISYVMTASQWKGKHFATVGVWRSIQSLALKNHDKVHAVITEGNTPSEQVFARLGF
                     RRLAPIE"
     gene            complement(6346..7278)
                     /locus_tag="VFQ23_17180"
     CDS             complement(6346..7278)
                     /locus_tag="VFQ23_17180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9908385.1"
                     /translation="MKITYSIVAPIYNERENLHELYRRIKEVMDSTNEPWELILVDDG
                     SADGSTEIIRELARKDKQIRPIIFARNFGHQIAITAGWDYARGDAVIIIDADLQDPPE
                     LILEMAKKWREGNEVVFAVRTEREGESWFKLWTASLFYRMIYRITDVKIPLDAGDFRL
                     MDRKVVNVLKQMRERHRFPRGMSAWVGFKQVGIEYKRAARVAGETKYPFRKMFRLAIN
                     AVTSFSYFPLQVATFFGFISAGIAILAIPLVAYMRITGSQAFFGQASTLIAVLFLGGV
                     QLISLGILGEYIGRLYDEAKGRPLYIVREAPEDN"
     gene            complement(7284..8549)
                     /locus_tag="VFQ23_17185"
     CDS             complement(7284..8549)
                     /locus_tag="VFQ23_17185"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9908386.1"
                     /translation="MNKDSQWSLTFRYVVGILLFLAVVAFVFYARNAMRNLVIAAFIA
                     YLINPAVEYLSKRMIMTRTAAVNFVYFSSLILMVGVPATLAPIFYDEAQIVVEDLMNL
                     STQVSSTLSQPVRIFGLVFHLEEWAQSLNQVQGAFLTPLPAEALHLLETTSVGVLWFL
                     IILVSVHILLSEWPNMREKLFSFVQEPYRAEAQELYRRVRRIWMAYLRGQIVLMIIVG
                     VVFTIAWLIMGIPGALVLGVIAGLFTLVPDVGPFLAVVIAATVALLEGSTWIPLSNFW
                     VAGILIVVYLILINLKNFFLRPIIMGRSVHMNEALVFIAILIATILEGVLGALLVVPV
                     LASAIVIIGYIQRRLIGLPPFEGDGSTQFVAPPEKINPPRREWRRKTHKKQSDSQSAI
                     SAESTPDSFESETNLQMEPKIDPNPAQEN"
     gene            complement(8546..9820)
                     /gene="thrC"
                     /locus_tag="VFQ23_17190"
     CDS             complement(8546..9820)
                     /gene="thrC"
                     /locus_tag="VFQ23_17190"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9908387.1"
                     /translation="MNKFVGYRCSLCGEEYSPVDVTYTCPKDGGNLDVVLDYDAIKNK
                     YQPEDLLSRTDPSLWRFLPLLPVSEPEGESTPLRAAGGTPVFELTSLAKKLGLQNLWL
                     KDESRNPTASFKDRASAIVVTRAQEIGAEVVVTASTGNAGAALAGMAAAVGQKAVIFA
                     PKTAPKAKVAQLRIFGAKVLLVDGTYDEAFDLAVSAAHEFGWYCRNTGYNPFTTEGKK
                     TAAFEIWEWWETTHRTWHEQFGTDVDHAPLTIFVSVGDGNIISGIHKGFKELYQLGWI
                     ARLPRIIGVQAEGSAAISNAFHQNTETITPVSSKTIADSIAVDLPRDGVRAVRAVKES
                     GGTYVNVSDEEILSAIAELGRVGVFAEPAGAAAYAGLVKGIGSGVVNRHDPILVLNTG
                     SGLKDIGAAMNAVSAESAPIIKPTLKSVKRVL"
     gene            complement(9925..11160)
                     /locus_tag="VFQ23_17195"
     CDS             complement(9925..11160)
                     /locus_tag="VFQ23_17195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9908388.1"
                     /translation="MNFEQFNLNSRLMKGINYAGYQTATPIQEAAIPAALRGRDIIGT
                     AQTGTGKTAAFVLPILNKLLDGPKGMPRALIVTPTRELAEQINQVVHALSTGTGVRSA
                     TIYGGVGADRQIKALRQGVEILVATPGRLLDLMQQRHAKLDRIEVLVLDEADRMFDMG
                     FLPDVKRIIKAVPVARQTMLFSATFPAEVELLAAQTLNNPQRISMGISRPAHTVTHAL
                     YPVPSHLKSALLLRLLKQIDSDSVLVFTRTKHRADKVARQIAHAGLRVTSLHGNRTQG
                     QRERALHGFKTGHFPIMVATDIAARGLDIEGITHVINYDMPDTADAYIHRIGRTGRAQ
                     RTGDAFTLVTPEDNDMIRSLERIMGGPIKRETLADFDYSPPAPPRHSSERREGSRPPR
                     PAPAMTSFGRNRLAPRKRR"
     gene            complement(11483..12154)
                     /locus_tag="VFQ23_17200"
     CDS             complement(11483..12154)
                     /locus_tag="VFQ23_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9908389.1"
                     /translation="MNKTITENRYVQILSMGVRLEGIVSIPDDAKGFVVFVHGSGSSR
                     HSPRNQYVAQTLQEGGMATLLFDLLTAEEEELDLRTRQWRFDITTLATRTAAVLEWLD
                     LQPFAYGLKKGLFGSGTGAAAALMAAAELPELVAAVVSRGGLPDLAGQKLAEVEAPTL
                     LIVGGRDQTVIDLNEQALSQLQPGLGAKMIVVTGATHLFEEPGALEEAAHLSRDWFQR
                     HLSIV"
     gene            complement(12461..13072)
                     /locus_tag="VFQ23_17205"
     CDS             complement(12461..13072)
                     /locus_tag="VFQ23_17205"
                     /inference="COORDINATES: protein motif:HMM:NF017519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HET9908390.1"
                     /translation="MIYTYQIEGIPVQTGDIICTKNGKPDILPGEFWLLVGRLVPGDV
                     DHVAIYTGPQGRCVEAGTHGVIKYTVFDGQWNTERMARRRGHLFDTFYGVVSPLDALG
                     LAEEEEREMRSKVAAYSLAQVGKPYNINFLNAETEDSFYCSQLVYKAYQQIGIDLNTG
                     LAMEQIPGTNTIIYPQEIWDGFNHRAANTAMLTNKQLVTNPPA"
     gene            complement(13090..14163)
                     /locus_tag="VFQ23_17210"
     CDS             complement(13090..14163)
                     /locus_tag="VFQ23_17210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9908391.1"
                     /translation="MKIGLIIYGSLDTLSGGYLYDRKLVEYLRSAGDTVEIISLPWRN
                     YAAHLTDNFSFRLPSHLDILIQDELNHPSLIFANRGKHSYPVISLVHHLRCSELRPKW
                     QNMYYRWLEKMYLQSVNGFIFNSKTTKQVVTDLIGNEKASAVAYPPTDRFKNVIAEDE
                     IFERAKTEKLNILFLGNVIERKGLHTLLEAMTHLKRDGNSLYHVNVVGSLNSEPEYAR
                     DIQQKVDICGLKSDVAFHGSLDKTPLIEKLKQAHMLVVPSSYEGFGIVYLEGMGFGLP
                     AIGTTAGAAREIITDGVDGFLIEPGNADLLAARLRSLNEKRDMLAQMSLAARDRYLRQ
                     PTWEESARQIREFLTKRIAEFTV"
     gene            complement(14176..14553)
                     /locus_tag="VFQ23_17215"
     CDS             complement(14176..14553)
                     /locus_tag="VFQ23_17215"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET9908392.1"
                     /translation="MYTLGVKRDFIARHYLIGGDWGSENFPNSHHYVLELQLSGKDLD
                     KHGYLVDILDVEKHLDAIVDYYKEQMLNDKPEFAGLNPSIEHFARILATSLSERINAE
                     NITELKVVLWENESAWAAYTLER"
     gene            complement(14567..15253)
                     /locus_tag="VFQ23_17220"
     CDS             complement(14567..15253)
                     /locus_tag="VFQ23_17220"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HET9908393.1"
                     /translation="MKFHYTNLDTCHEATGVVVVIDVIRAFTNAAFAFSRGAKEIYPV
                     SKVGEALQFKAEIPNSLACGEEGGIPPEGFDFGNSPTQTKTLDLQDKIIVQRTGAGTQ
                     GIVRSVKADTMLAASLVVAGATVKKIETLNPEAVTFVITGQYAPEHGDEDLACAEYLE
                     SLLKGEQPDPAPYVERVFRSRDAIMHLDPDQPAFPRSDLDHCTHIDAFDFVMLVSKEN
                     GRPILRAVKP"
     gene            complement(15250..15768)
                     /locus_tag="VFQ23_17225"
     CDS             complement(15250..15768)
                     /locus_tag="VFQ23_17225"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9908394.1"
                     /translation="MSINTKFEQAGFWERVKAFGLDYVLILFYLIAITLVIFLLNSLF
                     HINGLMFSERVRAQFFAFLLVTLPVTLYFSINESSKQQATWGKKRLSLQVVDANGGRI
                     SPGRAFTRTLLKFIPWELSFSPQANSIWISLGFGSVYVLIGLNIVSLLRTKSHQTVYD
                     LLTGTYVIKQNL"
     gene            complement(15765..16805)
                     /locus_tag="VFQ23_17230"
     CDS             complement(15765..16805)
                     /locus_tag="VFQ23_17230"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9908395.1"
                     /translation="MKRKTLYFTSPRQVELREESLPAPGANEVLVQTICSAISAGTEM
                     LIYQGHFPRDLETDTTISALRGGFKYPCMYGYASAGIVKEIGSEVGAEWLNRLVFAFQ
                     PHTSHFISKPELLYLIPESLSCETACFLPNMETAVNLVQDAAPLLGERILVLGQGVVG
                     LLTASLLNEFPLQSLVTADPYELRRQASIEIGVNKSLNPENKKPGNQNVPKEDNKALI
                     AYTQNNFDLVFELSGNPSALDDALSLTCFSGRIVIGSWYGEKKTEVSLGHAFHRSRIK
                     LISSQVSTIAPELSARWDKARRFEVAWQALERIRPEKWITQRFPFERADEAYRLLDEK
                     PQETIQVLLTYP"
     gene            complement(16822..17850)
                     /locus_tag="VFQ23_17235"
     CDS             complement(16822..17850)
                     /locus_tag="VFQ23_17235"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9908396.1"
                     /translation="MNSPSPLSMDAAGTNKKTNPLTVIRAAILYLILGALLYFALRNA
                     PLHEIWKTIQQLRFWQIALLFALNLFIYALLTLRWWVIVRAENKLVGYISLLVVRIAV
                     FGVSYFTLGPHVGGEPLQVFYLQRKYKFTYMHATASVILDKLLELLANFFLLALGLTA
                     VLQAGILSANGSVSWLSLSGLIILCLWPPLHITLLYKRIYPLSAALRSISFIKKDSKA
                     VRFVAASERLAGTFCRRHFFSLLSAIFISLLAGAGMVSEFALIAYFLNIHLPFWQTVS
                     AWMAGWLSLLAPLPGGLGALEASQVFALGLFGITATSAISLTLLMRGRDILIGGIGLL
                     LAGRTVRK"
     gene            complement(17847..18770)
                     /locus_tag="VFQ23_17240"
     CDS             complement(17847..18770)
                     /locus_tag="VFQ23_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908397.1"
                     /translation="MINEHYTSQRITMLPSNVSALTQINLDDLVASFGWQERPWLSRL
                     SRKLFAKPAQTFAHQMADFDSALGTYGLVEASRLALRNYVREIHVFDRDRIPASGFLA
                     LSNHPGMSDTLSLFAALNRLDLKIIAFQRPFLEALPYTSNQLFYLNDDPSSRIALIRK
                     VSTHLRNGGAALTFPAGHIEPDPDIHNDAVESLASWTDSAGVFIRLSPETPILPVLVR
                     GVVWAKAAYHWLTRINKTKREQEKLAAALQLLAYTALKVKPVSARVQIGKPIYAKELG
                     STETKVIHQAVMRALTQLIENPPKGEGERLL"
     gene            complement(18797..19564)
                     /locus_tag="VFQ23_17245"
     CDS             complement(18797..19564)
                     /locus_tag="VFQ23_17245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET9908398.1"
                     /translation="MSLQRLIAPIQRTLGFSFSFLIYYGLPFHHRALTEIYAPFIRPG
                     DLCFDLGAHLGDRVRAWSKLKARVIALEPNPASMSWLRRFYGNRENITLLEQAIGARA
                     GKADFWISRLTPSISTLSHQWLTTVQESARFSGAHWDEQIIVTVTTLDALIQQYGKPA
                     FCKIDVEGAELEVLQGLSQAIPALSFEYIPAAIETAVGCIDRLSQLGNYEYNWRVSEF
                     PRLRSRHWRRPDDMKAQLQQMPRDGNSGDVYARLISN"
     gene            complement(19607..19918)
                     /locus_tag="VFQ23_17250"
     CDS             complement(19607..19918)
                     /locus_tag="VFQ23_17250"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET9908399.1"
                     /translation="MELQVAPEIEIIQEQEEETELEPLYRVIIHNDDVTPMDFVVQIL
                     KTIFYLSNPKAAEVMLTAHYYGTAYVQTLPRSEAERRINKAHFAAGLEGYPLHFSLEP
                     E"
     gene            complement(19968..20786)
                     /locus_tag="VFQ23_17255"
     CDS             complement(19968..20786)
                     /locus_tag="VFQ23_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9908400.1"
                     /translation="MNFESIHGVTLPKLGFGTWKIGGGSYADPKLDSASLTALRTALE
                     VGYTHFDTAEMYGNGHTEELLSQAIRQSQLDREKLFITTKVTPSHLKYEDVLRACETS
                     LKRLKMEYIDLYLIHWPNTGMNLKDTFRALNKLVSDGQVRYLGVSNFNLRLLKESQSH
                     SATPLITNQVPYSISDRSYVRNGVLEYCQQHDILLTAYEPVDKGHLNISRTLQSIADA
                     HEANPYQIALAWLMAQPRVITIPMSYNPAHIKENFESVNIQLTGEEMKTLSGLS"
     gene            complement(20804..22126)
                     /locus_tag="VFQ23_17260"
     CDS             complement(20804..22126)
                     /locus_tag="VFQ23_17260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9908401.1"
                     /translation="MLETIGAWIRPPTFEGDEDKTRSATLLNIIIWVFIIGSVLYGLL
                     APIEPALRYRRAIIIVPFVLVLLVLKQLVHRGYVRPIGTVIVFALWGVITFAMFYGAD
                     YDNPAFMGYLVVVICAGLILSSRAAIGWSLFSILTSAVFLFLGQNNVLPASTPQSPMA
                     FWAAQTLYILVSTILISQSMRKIDEALARAQRELNERKRVEAEREKVIRELESKNAEL
                     ERFTYTVSHDLKSPLITIGGFIGLLEEDARSGNTVKFENDLRRIREAKDKMHHLLNDL
                     LELSRIGRLMNPPADIPFATIVEEAIELTKGRLMAANTQLKVQADLPVVRGDRARLVE
                     VMQNLIDNAAKFSVDQPEPRVEIGIQEDNKAIFFVRDNGIGISPKYHQRIFGLFDKLD
                     PTSDGTGVGLALIKRIIEIHGGRIWIESEKGQGATFYFTLPLGDHEIS"
     gene            complement(22281..23570)
                     /locus_tag="VFQ23_17265"
     CDS             complement(22281..23570)
                     /locus_tag="VFQ23_17265"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HET9908402.1"
                     /translation="MNNSNRLIKLIVTCTLLVMIIASTPAFAKSQASLDPTTQFYVPK
                     PNHGAKEQIADLTSSGNRTDANLIRQMIETPQAVWFTQGTPRSVLQDVRNTVQRAAAK
                     GTVAVLVAYNIPFRDCAQFSAGGATNAAEYKAWIDGFAAGIGSEQAVVILEPDGLGII
                     PWYNPFANRDTWVTNPNYEWCQPAEANQATAAADRFAMLNYAVDKLKENPSTSVYLDG
                     THSAWLGSGDSADRLIQAGVADADGFFLNVSNYRLDTHLEKYGTWTTKCIAFASNPAS
                     WGNGHSEWCASQYFPANPNDFSTWGQTDQWYTDNVESQTWWYSESLLKHFVIDTSRNG
                     QGPWTPTVSYPDPQEWCNPPGRGLGLRPTANTGNSLIDAYLWVKIPGESDGECTRGLG
                     PGGTTVDPEWGLIDPSAGHWFAEMALELAQNANPPLP"
     gene            complement(23837..25951)
                     /locus_tag="VFQ23_17270"
     CDS             complement(23837..25951)
                     /locus_tag="VFQ23_17270"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9908403.1"
                     /translation="MKKLIDKRPTIGVLSGIQVFYGTILGNFIGPLLHGVCSAAENRE
                     CNLLLACGMNASSYTTARPAWPVSSPDVDFVPVGPWNTDGLIVVNPLFSEARSGYIQE
                     LIAAGHPVVFAATGECGPTVAIDNVGAVQEALQHLTSHGHRQIAFIAGYPNDSTGDSG
                     IRLNAYQKIVQEHGLSSDSKLIAYGSHGINGGQKAMREILDGGAPFTAVLASNDESAI
                     GAMSVLKAAGLRIPEDVAIIGIDDTFEAITQVPPLTTFHSSPFQMGYQALELLLDTIE
                     GRRKELEDITIPMQFVVRQSCGCQPGSISQFVISKSETAISSKDTSSIVSELAKTMAD
                     IALSGTQRLGPDEVQSRCHHLIESFRESLKSKNPVGFRSSLEDTLRRVETLGDDVYVW
                     NETLSVLENNMGLVRDVMQWPASDQQPEAMLAEARATIAQHMQRQHRHSTTHQIWTAD
                     QVGQLNARLLIALDESQVYEILASYLPQVGIQHVDVAFFEREAGSPVARCRLSQVSEQ
                     GVEVSFPSHEFPPQGLYNEPFRLILLPMAASEETPGFVTFYAADLEKCAHIVWQLVTF
                     LRVVHLYREAKRGHQLAETLPHVEASLIRTSTLRSETQRVVRKTIAYLHEHYMEPISL
                     EAAARHVNMSKEYLARCFRQEMGVTLVTYLNRYRINQAKSLLEKGEYNLTKIALETGF
                     SSSAYFSRVFRQEVGMSPRDYK"
     gene            complement(26056..26442)
                     /locus_tag="VFQ23_17275"
     CDS             complement(26056..26442)
                     /locus_tag="VFQ23_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9908404.1"
                     /translation="MTKPEFERLMQTIADGWNEGNARKAAGCFAENTTYVEPPDKQLY
                     HGRDELYEFFGGDQGTDIPMKMIWHHLAFNEAEQIGFGEYTFEMHGRYHGIVVVQVES
                     DLIKHWREYQYRTDLDWEEFTSNSRF"
     gene            complement(26469..26858)
                     /locus_tag="VFQ23_17280"
     CDS             complement(26469..26858)
                     /locus_tag="VFQ23_17280"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9908405.1"
                     /translation="MSKRLNISSGVKWEEIVGYSRAARIGNVIEVAGTTALDEEGNLV
                     GPDDPYEQTRYIIAKIEKALISAGAALSDVVRTRIFTTDISRWEEIGRAHGEFFKNIK
                     PASTMVEVKALINPEMLVEIEATVILQ"
     gene            complement(26965..27495)
                     /locus_tag="VFQ23_17285"
     CDS             complement(26965..27495)
                     /locus_tag="VFQ23_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908406.1"
                     /translation="MPNKKIKINRAPVLTLWTAIVAERMGYKKDEALTFGKAVAGLNA
                     QSKVRRLGIYEEKSEEEKDKETKKEKPVKTEIVEMLGRGVPAIKTKQGLRAAIKGEEI
                     DAESVEKYLKQKFGEDLEAARAAMEKLAKAYPPKQLEKTAYSLYEKFRPKIPEGTKGW
                     GAKGELDLEYITSLAK"
     gene            complement(27573..>27935)
                     /locus_tag="VFQ23_17290"
     CDS             complement(27573..>27935)
                     /locus_tag="VFQ23_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908407.1"
                     /translation="LLGLGLGELSEDMPEAREHILQSLRLRQERGEQLPQTSSLIGVA
                     GLLLHAGNPQFAAQLLGAVASALKSLNAVMEPDIMHFHAQTLTAIRAQLTEAEFQSAW
                     ETGSEWSLEEAVRIALEG"
BASE COUNT         7155 a         6998 c         6700 g         7082 t
ORIGIN      
        1 tttatcctcg acccggcatt tgaccgatcg tccacgcgcc gaaagaactt tctttacgaa
       61 gggcttcacg ccctggacaa agatctgcgt tcgcgcggct cgtatctcgt gattcgcaat
      121 gggagaccta tcgctgtatt acaacaatta ctcacgcaga cacaggcaag cgccatttat
      181 gcggaggaag atttcacccc ctacgcaata aaccgtgacg agatgattgc acgccatctt
      241 cccctggaat tgattcaggg acaaaccgtt catcatcctt cttccgtctt gaaacctgat
      301 ggcaagccat acacggtcta tacgcctttc tccaaaacct ggaaagcaaa actccccttc
      361 ttgattgagc tgtttcccat acctgaccaa atcaagacac cgtcaggttt ttcgacggag
      421 cctcttcccg cgtttaaagt gaatccgctt ttccctgcgg gcgaacagga agcgctggtt
      481 cgacttgaag aatttcttca taaaagaatc ttttcttaca gcgaagaccg caaccgcatg
      541 gatttggatg gtacatctgc attatcgccg tatctgcact tcggaatgct cgggctgaga
      601 caagccgtca gtgccgcgcg gcaagttatg ggcggtgtcc tgagcttgtc gaaagatcaa
      661 aaaagaagtg gtgccgcaga aacatggctc aatgagctca tctggcgcga gttctttatc
      721 cagattctct atcatttccc ctatgtcagc cagcggtcct ttaatccatc atttacaaat
      781 atccaatggc gaaataacga agaggaattc aaatcctgga aaacaggccg aactggcgta
      841 ccgatcgtag acgcaggcat gcgtcaactt aaagaaatcg gctggatgca caaccgggca
      901 cgcatgatcg tcgcttcgtt tcttgttaaa gacctgctga tcgactggcg ttggggcgaa
      961 gcctggttta tggaaaacct tttggacggt gacctcgcgg caaataacgg tggctggcaa
     1021 tggacagcag gtacaggcac cgacgccgcg ccctacttcc ggattttcaa tcccgtattg
     1081 caaagcagga agtttgatcc gcagggagat tacatccgca aatggatccc tgagctgaga
     1141 gatgtagacg cgaaagatat ccatgcgccg tgggaagcag gaatcaaggt aaaaggctat
     1201 cctgaaaagc cgatcattga acgcgataaa gaaagaactt tgttagcgta taagatgtcg
     1261 aaagaggaac tcagagcgtg atggaaaata aacgtgacct ttccgtccct atgtcgcggg
     1321 caaacgttat tgtgatgatc atcgtccttc cggtagtgat cattcaattt gtgggtttca
     1381 ttgtgctgaa tggcctggat ggattgggaa ttacctgggg ttatccggtt cttcttatcg
     1441 cgatagtgct gggcgtggtt ctgcatgaat tgattcatgg cctttcgtgg gttattttcg
     1501 gaggcaaacc attttctgcg gtcaaatttg gctttcaatg gaaaagcctt acaccatatg
     1561 cacacctgaa agaacccgtg gaagtaaacc cttatcgaat cggtggcttc atgccctggt
     1621 ttgttcttgg gattctcccc tatttcatca gcctcgcctt gggcaatgga aacctgtttt
     1681 ggttcagtct gatacacaca tctgctgcag gtggagattg gctaattctt tggttgattc
     1741 gtaatgtaaa gaccgggaca ttggtggaag accatcctac aaatgcaggg tgttatgtaa
     1801 tcgagccgga aacggcaaca cgatagggaa aaatgaaaga ggcaaaagtt ctaattcgat
     1861 cgcttgtgga aattgatatt ccagaaatcg caagagcctt tgagatcatt ggctggaaca
     1921 aaccgatcgc gcaatatgaa cgatattacc gtgaacaggc acataaggct cgcgatgtct
     1981 atgttgcctt cgtcgatggg cagtttgccg gttaccttac aatttgctgg acctcgaact
     2041 acggaccgtt tcgagaagag aacatccctg agattgtaga tttcaatgtt ttgccaaaat
     2101 ttagaatgca gggcattggt gcccagctga tggataaagc agaaaatgag attgcaaaag
     2161 tgagttctgt ggcaggaatc ggtgtcggca tggatccaga ctacggcgcg gcccaacgct
     2221 tgtatgtgaa acgaggctat atcccggacg ggcgcgggct ctattacaga ggtcatcacc
     2281 cctcaggggg tgagaagatt acgatgggcg atgatttggc gttgtattta acgaaaaagc
     2341 taaagtagcg tatgacaatt agaagtattc accctgaaac cgggcgcgca gcactccgtt
     2401 cgctcatgaa ggaccgatcc ccgctggggc cattaagagt catggcaaag catgtcggac
     2461 gattttttca aatccctttg ccgatgttca aaccctttgt ggtgtttgga cccgaggcaa
     2521 atcgcaaagt actggtgact gagcgagata aaatgttgtg gcgaaataca gacccggtca
     2581 cggatttgct gggacgcggt gttctagttg tggatgggga ggagcatgat cactatcgca
     2641 aattgatgga acctccactt catccatcta cctttcacag ctacacggac atcatgatcc
     2701 gacagacaga ccgcgtctct tctttatgga aagagggcga gattgttgat atgctggtcg
     2761 agagccgtaa gatcgctttg ctgattatta tgcaaacgct tttcagcaaa gatgtgtggc
     2821 atgatctgcc aaagatatgg aaaccggttc tgaaatccat tgaatacata tcgcccgggg
     2881 catggatctt gtggcgcaaa atacctcgcc caggctttca caaaccatta caagcattgg
     2941 atgagtattt gtttggcatc attagagaaa gaaagaacgg aaataaagaa catgacttgt
     3001 tgcaacatct gattgacgcc gggttatcag acaaagtgat tcgcgatcaa atgttgacca
     3061 tgttgattgc cgggcatgat acttctactg cactgctggc gtggatattt gctttattgg
     3121 gccagcaccc tgaggtatat aaacaactag accaggaagt caatttgatg gaaaaatcgc
     3181 ctttattaga tcaggttatt aaagagagtt tgcgcctcta tccgccgatt cacattggca
     3241 atcggcgtgt tgcgcaggaa ctgcaacttg atgaaggcaa cgttccttct ggtgaacgcc
     3301 tgttttactc aatttatctt acacaccgcg atcctgcgat ctgggagaat gctgatgtat
     3361 tttgcccgga gcgcttttca catggcaaga aaactccgcc gttttcttat gtcccgtttg
     3421 gtggtggacc acgggtttgc atcggtgcgg tatttggtca ggcggaggcg cggattgtgc
     3481 ttgcgcggct gttgcaaacc tatcatttcg aattcacgaa tcataagatc cgtgcacaca
     3541 tgggcgcgac gctcgaaccg cggcccggcg tctttatgaa agttacaaaa agaaaagtaa
     3601 taaaaccaca aatttagaag acacaatgac ttactttggc tttctccttc gctttttatg
     3661 tatcccaatc ctgatatttc tagccattac atattgggat aataaaaatc atagacaaac
     3721 ccatggtttc cgaaatggtc gaggggtatg ggtagcaatc ggcgtccata tcttactcgc
     3781 ggtggtttac accacgccct gggataacta tctcgttgca acgggtgtgt ggtattacaa
     3841 ccctgaactg gtcacgggcc tattaatcgg ctatgtgccg atcgaggaat acacattctt
     3901 tgttttggaa accgtcttat ccggactatg gtggtggttt ttggctcggc gcgtttcccg
     3961 tctcccagag ggaaaggggc taggggtgag ggaaggtttg tggcccaaca aactaattta
     4021 tctatcttcg ggtgtactga ccttactatg gttaatcttt accagtctct tcttcttcgg
     4081 cgactccaaa tggacatatc tctccattac cctcttttgg gcactgcctg cgatcctgcc
     4141 gcagctttta tttggtgcag atatcctctg gcatcatcgc agacttgttt tattggggat
     4201 tcttattccg gggagctatc tctcgctgat ggacattttc gcgttacgtg aaacaacctg
     4261 gtctatttct ccgtcacaaa caacaggtat tctattcttc ggaattcttc cattggagga
     4321 agttgttttc tttttcatca caaatgtatt gattatcttt ggtatgactc tgctgctatc
     4381 cagcgtcagt caggagagat ttgcagaaat caaggaacag gtgcggggtt ggaaaaagaa
     4441 gcgtgtcgtc aatccgcagg gacgttgacc agatcgtgaa tccatttgcg tgagagaaat
     4501 cgccgaacaa taataaacgc tttgaccacc tcttcaatca acaccatggc atacacaccg
     4561 taaaccggta aatggaaaac aaagccaccc agaagtgcac tgggtacacc gaccagccag
     4621 acggaaaaaa gttcggtgaa catggcatag cgcgtatcac ccccggcacg caacgctcca
     4681 atgaacaaca taaaattgaa gacacgcagc cagaccgaga gagaatatac cagcataatc
     4741 caacgcaaat tttcaacggc agtaggggag agatcgtaaa gaccaattac ccgctcgcgg
     4801 agcaggaaaa cgataaaccc tccagcgatg gcggataata cccccaggat tgcaaagcgg
     4861 cggctgtact cgaatgcaag gtcttttttc ccttcaccga tcttgttgcc caccatcact
     4921 gaacaggcat tccccagccc gatgaacagg acaaagccca attcttcaat tgttgcattg
     4981 atgttcacgg cagcgatagc atccgtgccg atgcgcgcgt aaacggcgtt ataggttgtg
     5041 atgccaaagg accataacac ctcattggca gccgcgggca tcgatgtcgt aagcactttg
     5101 cccaggaagg cccggctgaa ctgaaacaat gtgaagaagt tggcagcaag cggcgtcttg
     5161 agaacataaa ccaatgtaac taaaagtaca cattcaaatg cccaaccaag actggtgcct
     5221 atggccgccc ctcgcacacc caatgcaggc aaaccaccaa ttccaaagat taatagatag
     5281 gcaacacatg ttttaagtac gagggcgagc acagaaacca ccacggttaa tttcacgaac
     5341 gtgatactgc gcaagagtga aatataagaa atcgatattg ccatcatcac atagcttaag
     5401 ccgacaatgc gtagataatc gctgcccagc ctgatgacct caggatcctc ggtataaaac
     5461 atcatcaatt tctcagggat aagcgtcgct gccagcgaaa aaagcaggct tacggaaacc
     5521 gcaacgatca aacatattcc caatatgtga tggatctgct ccgtatctcc cttgccccaa
     5581 aattgcgcgg taaaaatgga catgccactc gtcaccccaa agaggaagag gatcaataaa
     5641 aagaagatct gatttgcaag tccgagcgcg gcgactgccg tctcgcccaa ctggccaacc
     5701 atgagcacgt cgatcatgtt tagtccagct gtaatcaatt gctggaagga aactggcacg
     5761 gcgatggtta gcatcgcgcg aaaaaaatct ttgtcgcgga aaaaagagag agccattttg
     5821 gaggcgttga ttttgtgact tattcgattg gggctaggcg ccgaaatccc aggcgcgcaa
     5881 aaacttgctc ggatggggta ttgccttctg taatcactgc atgaactttg tcatgattct
     5941 taagagccaa agactgaatc gagcgccata caccaaccgt tgcgaaatgt tttcccttcc
     6001 actgggaggc agtcatcaca taggatatga acggcgaatt tctgtctttc cagaaaccga
     6061 ccagggatgc gcaagccagg ctattttcca taaacccaag ccacgagctt tcgagccagg
     6121 cagagtgttg cgaagaaaaa taggattcca cttcacgtat ggaatcatcc atcgtttcgc
     6181 catcatagtc aatggtatcc cgataagagt caagcataag ctcagccagg atttgttggt
     6241 ctgccagaga aggattgcgc caggttgctt tagcaagcgt tcctggcgca gtcgttggat
     6301 tcaaatcttt tagctcgagc acatactcga atcgatgacg catgttcagt tatcttccgg
     6361 agcctctcgg acaatataca atggcctccc tttggcttca tcatataggc gaccaatata
     6421 ttcgcccagg atccccaatg aaatcaactg cactccaccg aggaaaagca cagcgatcag
     6481 tgtacttgcc tgcccaaaaa aagcctgtga gcctgtgatg cgcatatagg caacgagtgg
     6541 gatcgctaat attgcaattc cagccgaaat aaagccaaag aacgttgcca cctgtaaagg
     6601 gaaatacgag aagctcgtca cggcattgat cgccagacga aacatcttcc gaaatggata
     6661 tttagtttcg ccagcgactc gcgccgcacg cttatattca atgcctacct gtttgaagcc
     6721 tacccatgcg gacatgccac gtgggaagcg atgccgttcg cgcatctgct tgagcacgtt
     6781 cactaccttg cgatccatca ggcggaagtc gcctgcatcc aacggaatct ttacatcggt
     6841 aatgcgataa atcatccgat agaaaagtga tgcggtccat aacttgaacc acgattcgcc
     6901 ttcacgctcg gtgcgaacgg caaaaaccac ctcatttccc tcacgccact tctttgccat
     6961 ctcaaggatg agttccggag gatcctgcag atcagcgtcg atgataatca cggcatcgcc
     7021 acgtgcgtag tcccagccag ctgtgattgc aatctgatgt ccaaaattgc gagcaaaaat
     7081 gatcggacga atctgtttgt cttttctggc aagctcgcga atgatttcgg tggagccatc
     7141 tgctgaacca tcgtctacaa ggatgagttc ccagggctcg ttggttgagt ccatcacttc
     7201 tttgatgcgc cgataaagtt cgtgaaggtt ttcgcgttcg ttataaatgg gtgccacaat
     7261 ggaataagtt atcttcattt gttctaattc tcttgagcag gattaggatc gatcttgggt
     7321 tccatttgca ggttcgtttc tgattcgaaa gagtcgggtg tcgactcggc ggaaatggca
     7381 gactggctat ccgattgttt tttgtgagtc tttctcctcc attcgcgcct cgggggattg
     7441 atcttttcag gaggggcaac aaactgcgta ctgccgtctc cttcgaacgg aggcagtcca
     7501 atcaagcgcc gttgaatata gccgatgatc acgatggccg atgcaagaac cggtacaacc
     7561 aataatgctc ccaaaacacc ttcaagaatg gtcgcgatca gaatggcgat aaataccagt
     7621 gcttcattca tgtggacact gcgtcccata atgatggggc gcaggaaaaa attcttgagg
     7681 ttgatcaaaa tcaaatatac aacgatcaga atcccagcta cccagaaatt ggaaagcgga
     7741 atccaggtag aaccttcgag taaagccaca gtggcggcaa tcaccactgc aaggaaggga
     7801 ccaacatccg ggaccagcgt aaagagaccg gcaatcactc ccagaacgag agcacccggg
     7861 attcccataa tcagccaggc aatcgtaaaa acaacgccaa caatgatcat caatacgatc
     7921 tgtccgcgca gataagccat ccaaatcctt cgaacgcgtc tgtagagctc ttgcgcttcc
     7981 gcgcgatacg gttcctgaac aaaggaaaac aacttctcgc gcatgtttgg ccattcagac
     8041 aataatatat gcacactcac caggatgatc aaaaaccata agacccctac ggaagttgtt
     8101 tcaaggagat gcagagcctc tgcaggtaag ggagtcaaaa aagcaccctg gacctgatta
     8161 agactttgag cccattcttc caaatgaaag accaaaccaa atatgcgtac tggctgtgat
     8221 aacgtactgc taacctgggt tgaaagattc attaaatctt ccacaacaat ttgcgcttca
     8281 tcatagaaga tcggggcaag tgtagcggga actcctacca tcaggatcaa cgacgagaag
     8341 taaacaaaat taaccgcggc tgtccgcgtc atgatcatgc gtttcgagag atattcaaca
     8401 gccgggttga taagataggc tataaacgca gcaatcacca gatttctcat tgcattgcgc
     8461 gcataaaaaa caaacgcgac cacagcaaga aaaagcagga tccctactac ataacgaaac
     8521 gtcaaactcc actggctgtc cttgttcata gtactctttt caccgacttc aacgtgggtt
     8581 taatgatcgg tgctgactct gcagagaccg cattcatcgc agccccgata tcttttaatc
     8641 cgctgccggt atttaacacc aggatgggat catggcggtt gaccacacct gagcctattc
     8701 cctttacgag acccgcgtac gcggcggcgc cggcaggctc tgcaaacaca ccgactctgc
     8761 caagttcagc gatagcgctg agaatctctt catctgatac attaacgtac gtcccgcctg
     8821 attccttcac cgcacgaacg gctctaaccc cgtcgcgcgg cagatcgacg gcgattgagt
     8881 cggcaatagt cttagaggaa accggggtaa ttgtctcagt attttgatga aaggcattgg
     8941 agatggccgc cgaaccctct gcctggactc cgatgatgcg cggcaggcgc gcgatccaac
     9001 ctaattgata gagttctttg aatcccttgt ggattccaga aataatattc ccatcgccca
     9061 ctgagacaaa aatcgtgaga ggggcatgat caacatctgt gccgaactgt tcatgccagg
     9121 tgcggtgtgt agtttcccac cattcccaaa tctcgaaggc ggcggttttt tttccttcgg
     9181 tagtaaaagg gttgtaacca gtattccgac agtaccatcc aaattcatgc gccgcgctga
     9241 ccgctaaatc aaatgcctca tcgtacgtgc catccactaa cagcactttc gcaccaaaaa
     9301 tacgcaactg tgccactttt gctttcgggg ctgtcttggg tgcaaaaatg acagcctttt
     9361 gcccgacggc cgcagccatc cctgccagtg cagcacccgc attccctgtg gatgcggtga
     9421 caaccacttc agcgccaatc tcctgggcac gcgtgaccac aatcgcgctg gctctatctt
     9481 tgaacgaggc ggttgggtta cgggactcgt ctttaagcca aagattctga aggccaagct
     9541 tcttagccaa agaagttaac tcgaaaacgg gggtaccacc tgccgcacgc agaggcgttg
     9601 actcaccttc tggctcagaa accggcaata aggggagaaa acgccacagg gacgggtctg
     9661 tgcgagagag caaatcttca ggctgatatt tgttcttgat agcatcgtag tccaagacga
     9721 catccagatt cccaccatct tttggacagg tgtaggtcac atcaacagga gaatactctt
     9781 caccacatag cgagcaccga taacccacaa acttgttcat gggctgattt tacccgataa
     9841 ggaattagac cagacacatc tggtctaatt ctggagacct attaaataaa tttggaaaga
     9901 gaaatagaag gggaagcttg ttgcttacct tcgtttcctc ggtgcgagtc tgtttctccc
     9961 aaagcttgtc atggcgggag cgggacgcgg cgggcgagag ccttcgcgtc tttcactgct
    10021 gtgtcgtggt ggagcaggcg gcgagtaatc gaagtctgct aaagtctcgc gcttaattgg
    10081 tccacccatg atacgttcaa gagaacgaat catgtcatta tcttcggggg tgacgagcgt
    10141 gaaagcatcg ccggttcgct gggcgcggcc ggtgcgtccg atgcggtgga tataagcatc
    10201 tgcagtgtcg ggcatgtcgt aattgatgac atgtgtaatg ccttcgatat ccagaccacg
    10261 cgcggcaatg tcagtggcga ccatgatagg gaagtgacct gttttgaatc catgcagggc
    10321 tcgttcacgc tgtccctggg tgcggttgcc atgcagactc gtgacgcgta atcccgcgtg
    10381 cgcgatctgt cgcgccactt tgtcagcacg gtgtttcgta cgcgtgaaga ccagcacgga
    10441 gtctgagtcg atctgcttga gcaacctcaa taacagtgca gacttcaaat gtgaaggcac
    10501 cggatacagc gcatgtgtca cggtatgcgc cggtcggctg atacccatcg agattcgctg
    10561 tggattattc aatgtctgtg ccgcaagcaa ttccacttcg gcagggaagg tcgctgaaaa
    10621 aagcatcgtc tgccgtgcca caggcacagc tttgataatg cgcttaacat ctggcagaaa
    10681 acccatatcg aacatccggt cggcttcatc cagaaccaga acttcaatgc ggtccaactt
    10741 cgcatgacgt tgttgcatta ggtcaagcaa gcgccccgga gttgcaacga gaatctccac
    10801 accctgacgc aatgccttga tctggcgatc cgctccgact ccgccgtaga ttgtcgcgct
    10861 ccgcactccg gtccccgttg agagggcgtg cacgacttga ttgatctgtt ctgcaagctc
    10921 gcgcgtgggt gtgacgatca gagcgcgagg cattcccttc gggccatcca gtaacttatt
    10981 gagaataggt aagacgaacg ctgctgtctt gccagtacct gtctgcgcgg tgccaataat
    11041 gtcgcgccca cgtagcgcgg cagggatcgc cgcctcctga ataggagtcg cagtttggta
    11101 gcccgcgtag ttaatcccct tcattaaacg cgaatttaaa ttaaattgtt cgaagttcaa
    11161 aattgttcct tgtcttcaga agccatgcac ttgtaaagat tgagaattcc ctcaaaaaaa
    11221 cagcggtgga ctgctgatac taatgaattg ataagaagat tatacactag taatcccgac
    11281 gccattgcga gagatgggtc tcaatccgcc ttccctgtac gacaccacac ctgcctgatt
    11341 tgctcaagca aatcacgggc ggtgccaggg gggaacacac gatttgttcg gcaaagcgtg
    11401 tgcaagtgtc gctacaaact cttaagctta ccgggccacc agccctcgta gcgataaatc
    11461 gaaccgtgtt ccaattatta ttttagacaa tgcttagatg cctttggaac caatcccgcg
    11521 acaggtgtgc agcctcctcc agtgctcctg gttcctcaaa gaggtgtgtg gctcctgtca
    11581 caacaatcat ttttgctccc agtcccggtt ggagttgtga aagtgcctgt tcgtttaagt
    11641 cgatcaccgt ctgatctcga cctccaacaa tcaatagtgt tggtgcttct acctcagcca
    11701 acttttgtcc tgccagatca gggagtccac cccgtgatac aaccgccgca accaactcag
    11761 ggagttccgc ggctgccatc aacgcggcgg cagctcccgt gccggagcca aataacccct
    11821 ttttcaggcc gtaagcaaaa ggctgtaaat ccagccattc aagcactgct gcagtccgag
    11881 tagccagcgt agtgatgtca aaccgccact gacgagttcg tagatccagc tcttcttctt
    11941 cagccgtcag tagatcaaag agcaatgttg ccatgccgcc ttcctgcaat gtttgagcca
    12001 catattgatt gcgcggacta tggcgactgc tgccactccc atgtacaaac acgacgaatc
    12061 cttttgcatc atccgggatg cttacaattc cttccagccg tacacccatg ctgaggattt
    12121 gaacatatcg attttctgta attgtcttat tcattgaaaa atccttgtct gtgttgcaac
    12181 tgccgatttg agccctgtat attccaattt cttagccagt tgcttgcctg catccctccg
    12241 gtccctgaac aaagccttcg ctgcagcgtc ggtgtgaaac atttttttgt gggtcacttt
    12301 ctcttggctg aggagttcat tgaatgcatt gtgagaaaac tattaaaaca atataacctt
    12361 ccatgttggt tatggaaatc aggaatttcc ccaaccaggt agggtctttc ccactttcga
    12421 ttgggactct tatatgaaca aactgggaaa atatttgaag ctatgccggt ggattggtca
    12481 ctaactgttt attggtcagc atagctgtgt ttgctgcgcg atgattgaac ccatcccaaa
    12541 tctcctgcgg gtaaatgatc gtatttgtcc ccgggatctg ttccatcgct aggccagtgt
    12601 tcaaatcaat gccgatctgt tggtatgctt tatagacaag ctgactgcaa taaaaagagt
    12661 cttcggtctc agcattgaga aaatttatgt tatacggttt acccacctga gccagactat
    12721 aggctgcgac cttgcttcgc atttcacgtt cctcttcctc ggcgagccca agcgcgtcca
    12781 gtggagaaac cactccgtaa aatgtatcga ataagtgtcc gcgtcggcga gccatgcgtt
    12841 ccgtattcca ttgtccgtcg aagacggtat atttaatgac accgtgcgta ccagcttcca
    12901 cacatctccc ttgtggaccg gtgtagatcg ctacatggtc aacatccccc ggaacaagcc
    12961 gaccgacaag cagccaaaat tcaccgggca gaatatcggg cttgccgttc tttgtgcaga
    13021 tgatgtcgcc ggtttgtact ggaatcccct cgatttgata agtgtaaatc attctctctc
    13081 cgcaatggat tatacggtga actccgcgat tcgtttcgtt aaaaattcgc ggatctggcg
    13141 tgctgattct tcccatgttg gctggcgaag atagcgatct cgcgctgcca gactcatttg
    13201 ggcaagcata tctcgtttct cattcaggga cctcaacctg gctgccaaca aatcagcatt
    13261 tccgggctca atcaggaatc cgtcaactcc gtccgtgatg atttctcgcg cggcgccggc
    13321 ggttgtccca atcgcgggca gaccaaaccc catcccttcg aggtagacaa taccaaatcc
    13381 ctcgtagctt gaaggaacga cgagcatgtg cgcttgtttt agtttttcga taagtggtgt
    13441 cttgtcgagt gaaccgtgaa aagcgacatc agactttagt ccacagatat caactttctg
    13501 ctgtatatct ctcgcgtatt ctggctcgga attcaatgaa ccaactacat tcacatgata
    13561 aagcgaattg ccatctcgtt ttaagtgtgt cattgcttcg agcagcgtat gtaagccttt
    13621 ccgctcgatc acattcccca ggaataaaat gtttagtttt tccgtctttg ctctttcaaa
    13681 tatttcatct tcggcaatta cattcttaaa tcggtctgtc ggtggatagg caacagctga
    13741 agctttttca ttgccgatta aatcagtcac gacctgtttt gttgtctttg aattaaatat
    13801 gaacccattc acactctgca aatacatctt ctccaaccag cgataataca tattttgcca
    13861 tttcggtctc aattccgaac agcgcagatg atgaacaagt gaaatgaccg gatacgaatg
    13921 ttttcctcgg tttgcaaaga tcagagatgg atgattgagt tcatcctgaa tcaggatatc
    13981 gaggtgagat ggaagccgga aactgaagtt atcggttaaa tgcgcagcgt aatttcgcca
    14041 gggcagagaa ataatctcca cagtatctcc tgctgaccga agatattcca ctagtttgcg
    14101 atcatacaaa tatccgccgc ttaacgtatc aagcgaacca tagatgataa gtccgatttt
    14161 cattcgtagt acgttttaac gttccaacgt ataagctgcc cacgctgact cattctccca
    14221 cagcacgact ttgagttcag ttatattctc ggcattgatc cgctcgctaa gtgacgttgc
    14281 caatatgcgc gcgaaatgtt caatggaggg gttaagcccc gcaaattcgg gcttgtcgtt
    14341 cagcatttgc tctttgtaat aatccacaat cgcatcgagg tgcttttcca catccaggat
    14401 atccacgaga taaccgtgtt tgtccaggtc tttgcctgag agttgtaatt caaggacata
    14461 atgatgtgaa ttgggaaaat tctctgaccc ccaatcgccg ccgatcagat aatgccgtgc
    14521 aatgaaatcc cttttgactc ctaatgtata cataaaatct ctgatttcaa ggttttaccg
    14581 cgcgcaggat aggacgccca ttttcttttg atacaagcat gacaaaatca aacgcgtcaa
    14641 tatgggtgca atgatcgagg tcagagcgtg gaaaggcagg ctggtcgggg tcaagatgca
    14701 tgatggcatc gcgggatcga aagacgcgtt caacatacgg agcagggtca ggttgttcac
    14761 cttttagtaa ggattccaaa tattcagcac aagccagatc ttcatcgcca tgttctggtg
    14821 catactgccc tgtgattacg aaggtcacag cctccggatt taacgtctcg atctttttca
    14881 cggtggcacc cgcaaccaca agacttgcag caagcattgt atccgccttg acacttcgga
    14941 cgattccctg cgtccccgcg ccggtacgtt gaacgatgat cttatcttgc agatccagag
    15001 tttttgtttg agtgggggag ttgccgaaat caaatccctc cggcggaata ccgccttcct
    15061 caccgcaggc caatgaattt ggaatctctg ccttgaactg cagtgcctct ccaactttgc
    15121 tgaccgggta gatttccttg gcacctcgtg aaaatgcaaa ggcggcattt gtaaaggcgc
    15181 gaatgacatc aataactaca acaaccccag tcgcttcgtg gcacgtgtca agatttgtat
    15241 aatgaaattt catagatttt gttttatcac ataagttccg gtgagcaagt catatacggt
    15301 ttgatggctc tttgtccgga gaagactgac gatgttaagc ccaatcaata cataaaccga
    15361 gccaaagccg aggctgatcc aaatggaatt ggcttgaggc gaaaaggaaa gttcccaggg
    15421 gatgaatttt aatagcgtac gagtaaatgc tctaccaggg ctgattcttc caccattggc
    15481 atccaccacc tgcaagctca atcgtttttt gccccatgtg gcctgctgct ttgatgactc
    15541 attgatggaa aaataaagtg tgaccggaag ggtaaccagc aaaaacgcaa aaaactgcgc
    15601 tcgtacacgc tctgagaaca tcaagccatt gatgtgaaag agcgaattca gtaaaaaaat
    15661 taccagggta atcgcgatca aataaaataa gataagcaca taatcgagtc caaatgcttt
    15721 cactctttcc cagaaacccg cttgttcaaa ttttgtgttg atgctcatgg gtaggtcagt
    15781 agcacttgaa ttgtctcttg cggcttttca tccaacagac gatatgcctc atcggctctt
    15841 tcgaacggaa atcgctgtgt gatccatttt tccggtctga ttctttccaa ggcctgccac
    15901 gctacttcaa agcgacgtgc cttatcccat cgggcggata actctggggc aatggtgcta
    15961 acctgcgagg aaatcaattt gatacgtgag cgatggaacg cgtgccccag actgacttct
    16021 gttttctttt cgccgtacca ggagccgatc acgatccgcc ccgaaaaaca agtgagggaa
    16081 agggcatcat ccagagctga aggatttccg gaaagctcaa acacaaggtc gaaattgttt
    16141 tgcgtgtagg caatcaaagc tttgttgtcc tccttgggca cattttgatt tccgggtttc
    16201 ttattctcag ggttgagtga tttattaact ccgatctcaa tcgatgcctg gcgcctcaac
    16261 tcgtatggat ctgcagtgac cagactctgc aaaggaaatt cattcagtaa ggaagccgtg
    16321 agcaaaccga caacaccctg ccccagaact agaatgcgct caccgagaag cggtgcggca
    16381 tcctgaacca gattgacggc ggtttccatg ttggggagaa aacaagctgt ttcgcaggaa
    16441 agagattctg gaatcaaata taacaattct ggtttgctga tgaagtgcga tgtgtgcggc
    16501 tgaaaagcaa ataccaatct gttcaaccat tcggcaccga cttcgctgcc aatttccttc
    16561 acgattccgg ccgacgcgta tccgtacata catggatatt tgaagccgcc gcgcaacgcc
    16621 gaaatagtgg tgtccgtttc aaggtcacgc gggaaatgac cctgataaat cagcatctca
    16681 gtgccggcgc tgatcgcgga acaaatggtc tgcaccagga cttcattcgc gccaggcgcg
    16741 gggagagact cttcacgaag ttccacttgc cgcggcgacg tgaaatataa cgtttttcgt
    16801 ttcacttcct cattttacct tttactttcg tacagtacga ccagctaaca gtaacccgat
    16861 gccgccaatc aaaatatcac gtccgcgcat taggagcgtt aagctgatcg cagaggtagc
    16921 cgtaatacca aacaagccca gcgcgaatac ctggctggcc tccaacgcgc ccagccctcc
    16981 gggtaacgga gcaagcagcg aaagccagcc cgccatccat gcggaaacag tttgccaaaa
    17041 tggcaaatgg atgttcagaa aataagcgat gagcgcgaac tcactgacca tccccgcacc
    17101 cgccaggagg gatatgaaaa tggcagataa taacgagaag aaatgccggc gacaaaacgt
    17161 accagccaaa cgttcagatg ctgctacgaa gcgtactgct ttcgaatcct ttttgataaa
    17221 ggaaatgcta cgcaacgcag ccgacagggg ataaattcgc ttatataaga gagtgatatg
    17281 aaggggcggc cacaaacata ggatgatcag cccgctcaga cttaaccagg agacgcttcc
    17341 gttcgccgaa aggattcccg cctgaaggac ggctgtcaga cccagcgcga gcaaaaagaa
    17401 gtttgccagc aactcaagta atttatccag aataacggat gcggttgcgt gcatgtaggt
    17461 aaatttgtat ttgcgttgaa ggtaaaaaac ctgcagcggc tcaccaccca catgcggacc
    17521 aagcgtgaag taactgactc caaacactgc gattcgaacc acaagcaacg agatgtagcc
    17581 tacaagtttg ttttctgcgc ggacgatgac ccaccatctc aacgtgagta gtgcgtagat
    17641 aaatagattc aaggcgaata agagagcaat ttgccagaac cgtagttgtt gaatcgtttt
    17701 ccaaatctca tgcaacggcg cgttgcgcaa tgcgaaataa aggagcgcgc caagaattag
    17761 gtagagtata gcggcacgga tgaccgtgag cgggtttgtt tttttatttg ttcctgcggc
    17821 gtccattgaa agtggagaag gcgaattcac agcaatcttt ccccttcgcc tttgggagga
    17881 ttctcgatca attgagtcag cgctctcatc acagcctgat gaatgacttt ggtttctgta
    17941 gagcccaact cttttgcgta aatgggtttg ccaatttgta cccgcgcgct gacgggtttc
    18001 acttttaatg ccgtgtaagc cagtaactgt aacgcggcgg cgagcttttc ctgttcccgc
    18061 ttcgtcttat tgattctcgt cagccagtgg taggcggctt ttgcccatac aacgccgcga
    18121 accaatactg gcaggatcgg agtttcagga gacaggcgga taaatacacc cgcgctgtcg
    18181 gtccaggagg ctaatgactc tacagcgtcg ttatggatat ccgggtctgg ctcgatatga
    18241 cccgccggga aggtgagtgc cgcgccacca tttcgtaaat gtgtactaac ctttcgaatc
    18301 agcgcgatgc gggaagacgg atcatcattt aggtaaaaca attgattcga tgtataaggc
    18361 agcgcctcaa gaaatggacg ctgaaacgca atgatcttta ggtcgagacg atttaatgcg
    18421 gcaaagagtg agagtgtatc gctcatgccc gggtgattgg ataaagccaa aaaaccagac
    18481 gcagggatac ggtctcgatc aaagacgtga atctcgcgaa cgtaatttct cagcgcgagt
    18541 cgcgaagctt cgactaaccc gtaggttcca agcgctgagt caaaatctgc catttggtga
    18601 gcaaaggttt gggcaggctt cgcgaaaagc ttgcgtgaaa ggcgcgaaag ccagggccgt
    18661 tcctgccagc caaacgaagc aacaagatca tctaaattga tttgcgtaag agctgacacg
    18721 ttggaaggca gcattgtgat tcgttgtgaa gtgtaatgtt cgttgatcat actcgtttcc
    18781 atgcctggca gttgcgttag ttcgatatca ggcgggcgta aacatcccct gaattgccgt
    18841 cgcgcggcat ctgttgaagt tgtgccttca tatcatccgg tcgacgccag tggcgcgaac
    18901 gcaagcgagg aaactcactc acgcgccaat tgtattcgta atttcccagt tgactcaatc
    18961 tatcaatgca gcctacggca gtttcaatgg cggccggaat gtactcaaac gacagcgcag
    19021 ggatagcttg tgataagcct tgaagcactt ctagctcagc gccttcaaca tcaatcttgc
    19081 aaaacgctgg tttcccatac tgctgaatta atgcatccaa agtagtgacc gtaacgatga
    19141 tttgttcatc ccaatgcgcg ccggagaacc ttgctgactc ttgaactgtg gtcaaccatt
    19201 gatgtgaaag agttgaaatg cttggggtaa gacggctgat ccaaaaatct gctttgccag
    19261 cccgagctcc aatcgcttgt tcaaggaggg taatattttc acgattgcca tagaaacggc
    19321 gaagccagct catggacgcc ggattcggct ccagcgcaat cacgcgtgcc tttagcttcg
    19381 accaggcgcg cacgcggtcg ccgagatgtg ctcccagatc aaaacataaa tcgccgggac
    19441 gaatgaacgg agcgtagatc tctgtcagtg ctctgtgatg aaagggcaga ccgtaataga
    19501 tgaggaagga aaaactgaaa cccaacgttc tctgaatcgg agcaatgagg cgttgaagag
    19561 acataagaaa atttatcgtg tcaggcagcg ggcaggtcgc cctgactcat tcaggctcaa
    19621 gcgaaaaatg taatggatat ccttccaagc ccgcggcaaa gtgagctttg ttgatccgtc
    19681 tctctgcttc agatctcggc agcgtttgca cataggcggt gccataataa tgagcagtga
    19741 gcataacttc tgccgctttg ggattcgaca agtagaagat cgttttcagg atttgcacga
    19801 caaaatccat cggagtgaca tcatcattgt ggatgatgac acgatagagc ggttcgagtt
    19861 cagtttcttc ttcctgctcc tgaatgatct caatttctgg tgcgacctgt aattccataa
    19921 aaatatacga gttgattcca actatagatg cattactgaa aacgatgtta tgagagtccg
    19981 ctaagagttt tcatttcttc ccctgtaagt tggatgttta cggattcaaa attttccttg
    20041 atgtgggccg gattgtaaga cattggaatg gtaatgacgc gtggttgagc catgagccat
    20101 gctaacgcga tctggtaggg attcgcttca tgtgcatccg caattgattg caatgtgcgg
    20161 cttatgttta agtgtccttt atccacaggt tcataggcgg taagcagaat gtcatgttgc
    20221 tggcaatatt ccagcacacc attgcggacg taggatcgat cagagatgct gtaggggacc
    20281 tgattggtga tcaacggtgt tgcagaatgt gactgcgatt ctttcaacag tctcagattg
    20341 aagttgctga cgccaagata cctgacctgg ccgtcactca caagtttgtt taaagcacga
    20401 aatgtatctt tcagattcat gcccgtattt ggccagtgaa tcagatacaa gtcgatatat
    20461 tccattttga gtcgcttcag gctggtttcg catgccctca gcacatcctc atatttgaga
    20521 tgggagggcg tgactttcgt ggtgataaat agcttctcac gatccagttg cgattggcga
    20581 atcgcctggc tgagcaattc ctccgtgtga ccgttcccat acatctcggc tgtatcaaag
    20641 tgagtgtagc cgacttcgag agcggtgcga agcgcggtca gtgaggcgga atcgagcttc
    20701 gggtccgcgt aggaaccgcc gccgattttc cacgtcccga agccgagctt gggaagcgtg
    20761 acgccatgga tcgattcgaa gttcattagg tgtcattata gcatcatgaa atttcgtgat
    20821 ctcccagggg caacgtgaaa taaaacgtgg ccccttgtcc tttttcggat tcaatccaga
    20881 tgcgccctcc atggatttcg atgatccgtt taatgagagc cagtcccaca cccgtgccat
    20941 cactggtggg gtccagtttg tcgaacagac caaatatcct ttgatggtat tttggagata
    21001 ttccgatgcc attatctcgg acgaagaaga tcgccttgtt gtcttcctgt atgcctatct
    21061 caacccgtgg ctcgggctgg tcaacggaaa atttcgccgc gttatcgatc agattctgca
    21121 tgacttcgac caggcgcgcc cggtctccac gaaccaccgg cagatcagcc tgaactttta
    21181 gctgggtatt ggcggccatc aggcgccctt tggttaattc aatggcttcc tccacaatag
    21241 tcgcaaaggg aatatcggca ggcggattca tcaatcgccc aatgcgtgag agttccagca
    21301 ggtcattcaa caggtgatgc atcttatctt ttgcctcgcg gatgcgtcgc agatcattct
    21361 caaacttgac cgtgttccct gagcgcgcat cctcctccag caagccgatg aatcccccaa
    21421 ttgtgatcaa tggactcttc agatcatgcg acacggtata ggtaaatcgt tccaattcag
    21481 cattctttga ttccaattca cggatgactt tttcacgttc ggcttccacg cgtttgcgct
    21541 cgttcaattc gcgctgtgct cttgcaagcg cctcatcgat cttgcgcatg gattggctga
    21601 ttaagatcgt ggaaacaaga atgtatagag tctgtgccgc ccaaaaagcc ataggtgatt
    21661 ggggcgtaga ggcaggcaac acattgtttt gtcccagaaa cagaaacacc gcgctggtga
    21721 ggatgctgaa caaactccac ccgatcgcgg ctcgcgagct caggatcaac ccggcacaga
    21781 tcacaaccac cagatagccc atgaaagcag ggttgtcata gtctgcgccg taaaacatag
    21841 caaacgtgat cacaccccat aaggcgaata caatgaccgt gccaatcggc cgcacatagc
    21901 ccctatggac cagctgtttc aacaccagaa gcacaagtac gaacggaacg ataattattg
    21961 cgcggcgata tcttagcgca ggttcgatgg gtgccaggag cccatagagc acggacccaa
    22021 tgataaatac ccagataatg atgtttaata aggtggccga gcgggtttta tcttcatccc
    22081 cttcgaaggt tggagggcga atccacgcac caattgtttc gagcataagc actcctgaat
    22141 tgttccgaaa agaaaacctt gatgcttata caacaacttc cattttggcg caacataaaa
    22201 gagcaggaca aactgtcctg ctcttttata agttggagtc actgcactgt gcgtgtcaag
    22261 aggaagagga cgcccggtaa ctatggaagg ggtgggttgg cattctgcgc cagttcaagc
    22321 gccatttcag cgaaccaatg tcccgcactg ggatcgatca gaccccattc cggatcaacc
    22381 gttgtgccgc ctggaccgag accacgggtg cattcgccat ctgactcacc tgggatcttc
    22441 acccagaggt atgcatcgat cagagaattt cctgtgttgg ctgttggccg gagccccagc
    22501 ccgcgcccag gtgggttgca ccattcttga gggtcgggat agctaacggt tggagtccag
    22561 ggtccctgtc cattgcggct ggtgtcaatg acaaagtgtt tgagcagaga ctcagagtac
    22621 caccatgtct ggctctcaac attgtctgta taccactggt ccgtctgacc ccacgtgctg
    22681 aagtcatttg gatttgcggg gaagtactgg cttgcgcacc actcggagtg accgttaccc
    22741 caggacgccg ggttcgatgc aaaagcaatg cacttcgttg tccaggtgcc atatttctcg
    22801 aggtgtgtat ctaggcggta gttggacaca ttcaggaaga aaccgtccgc gtccgcgacg
    22861 cctgcctgga ttaaacggtc agctgaatcg ccggagccca accaggcact atgagtgcca
    22921 tcgaggtaga cacttgtgct cgggttttcc ttaagtttgt cgaccgcgta attcagcatt
    22981 gcaaatcggt cagcggcggc tgttgcctga ttcgcctcgg ctggctggca ccactcataa
    23041 ttcggattgg tcacccaggt gtcgcggttt gcaaaggggt tgtaccacgg gatgattccc
    23101 aacccatccg gctcaaggat gaccactgcc tgttcactgc cgatccccgc ggcaaatcca
    23161 tcaatccagg ctttgtactc tgccgcgtta gtagcgccac ctgcactgaa ctgggcacag
    23221 tcgcggaaag ggatattgta tgcgaccagg accgcaacag tgcctttggc agccgcgcgt
    23281 tggacggtgt tccgaacatc ttgcagcaca gatctcggag tgccttgcgt gaaccagact
    23341 gcttgtggcg tctcgatcat ttgtcggatc agattggcat ccgtcctatt tccgctggat
    23401 gtaagatcgg caatctgctc cttcgcaccg tggttgggct tgggaacata gaattgcgta
    23461 gtgggatcca ggctagcctg cgactttgca aatgctggtg tcgacgcaat gatcatcacc
    23521 aaaagcgtgc aagtaactat cagttttatt aaacgatttg aattgttcat ttttctcctt
    23581 ttacctcagg ttggcttttc gcggggtgag ttggactcac aagagtggaa ccttgagcat
    23641 cgctgcaatc tattcactat tagtatgcat cacacgatgc gaaaagtctt acaaaatatt
    23701 aatcagtata ggaaaatatt gaccaacttg aaaataacat aaaagagcaa gcaggattga
    23761 cctttgagaa ttgaaatcac cccgtcttat ctataataaa gaatgatgat tgtttgacaa
    23821 tctaagaatt tgcagatcat ttatagtctc ttggactcat tcctacttcc tgccggaaga
    23881 cacggctgaa ataagcactg ctcgaaaagc ccgtttccag ggcaattttt gtcagattgt
    23941 actcaccttt ctccaaaagg gattttgcct ggttgatgcg ataacggttt aaataagtta
    24001 ccaaagtaac acccatttcc tgtcgaaaac aacgcgcaag atattccttg ctcatattta
    24061 catgccgggc tgcagcctcc agcgagatcg gttccatata gtgttcatgg agataagcaa
    24121 tggtcttacg gaccacacgt tgtgtttcac tcctcaatgt cgaggtgcgt atcagactcg
    24181 cttcaacgtg aggcaaagtc tcagctagtt ggtgtccacg cttggcttca cgatacaaat
    24241 gaacaaccct taaaaaggtg acaagttgcc agacaatatg tgcgcatttt tccagatccg
    24301 ctgcgtaaaa agtgacaaat ccgggtgtct cttcgctggc ggccatgggg agcaagatca
    24361 agcgaaatgg ttcattatat aaaccctgtg gcggaaactc atgactcgga aagctaacct
    24421 caactccttg ttccgacacc tgacttaacc tgcagcgagc gactggactt ccagcttctc
    24481 tttcaaagaa agccacatcg acatgttgga tgcccacctg aggcaaataa ctggcgagta
    24541 tctcatagac ctgggattca tccagcgcaa tgagcaagcg ggcgttcaac tgacccactt
    24601 gatcggcggt ccatatttga tgagtcgtcg aatgcctgtg ttgacgctgc atatgctgag
    24661 caatggttgc acgggcctct gccagcatgg cttcgggttg ttgatcggag gccggccatt
    24721 gcatcacatc ccgaactaaa cccatattgt tttccaacac tgagagcgtt tcattccaga
    24781 catatacatc gtccccaagg gtctctacgc gccttagcgt atcctcaagg gaggagcgaa
    24841 agccaacagg atttttcgat ttcaggctct ccctgaatga ctcaatcaga tggtggcacc
    24901 gggactgcac ctcgtccggt cctaaacgct gggttcctga taacgcgata tcggccattg
    24961 tcttcgcgag ctcggagacg attgaagaag tatccttgct ggatatcgct gtttcagatt
    25021 tgctaataac gaactgcgaa atagagcctg gctggcagcc gcatgattgg cggactacaa
    25081 attgcattgg tatggtgatg tcttccaact cttttctccg accttcgata gtatccagca
    25141 gcaattccag cgcctgatat cccatttgaa atggtgagct gtgaaacgtt gtgaggggcg
    25201 gcacttgcgt tatcgcttcg aatgtatcgt caatacctat aattgcaaca tcctcaggga
    25261 ttcgaagtcc cgccgctttg agcacgctca tcgctccaat ggcagattca tcattgctcg
    25321 ccaatacggc agtgaatgga gcacctccat ctaagatttc gcgcatggcc ttttgtccac
    25381 cattgatgcc atgacttcca taagcaatca gtttcgaatc agaagatagg ccgtgttcct
    25441 gaacgatctt ttgataagca tttaagcgga tgccgctatc gcccgtggaa tcattagggt
    25501 aaccggcaat gaaagcaatc tgtctgtgac catgagatgt cagatgctga agtgcctctt
    25561 gcacagcacc aacattatca atcgcaacgg ttggaccgca ctcgccggtc gccgcgaaaa
    25621 caacaggatg cccggcagcg atcagttcct gaatgtaacc tgatcttgcc tccgaaaaca
    25681 gcgggttgac cacaatcagt ccatctgtgt tccacggtcc gacgggtaca aagtccacat
    25741 ctggtgatga taccggccac gccggtcgag ctgtagtgta agaggatgca ttcatgccgc
    25801 aggccaacag taaattgcat tcccgattct cggcggcgga gcacacacca tgcagcaaag
    25861 gaccaatgaa gttccccaga atggttccgt aaaacacctg aatgcctgaa agcaccccta
    25921 tcgttggcct tttgtctatg agctttttca ttagcaagat cctttatcgg ctctgtcgtg
    25981 cttattatac gagtaactta aacacaaaga acctgaccct cacacagcca ggtgatttag
    26041 ccctaaaact ttgacctaaa agcgactatt gctcgtgaac tcttcccaat ctaggtccgt
    26101 tctgtattgg tactcccgcc agtgtttgat aagatcagat tccacttgca ccactacaat
    26161 gccatgatag cgtccgtgca tctcgaacgt atattctcca aaaccaatct gttccgcttc
    26221 attgaaagcc agatgatgcc agatcatttt catcggaatg tctgtaccct gatctccgcc
    26281 gaaaaattca taaagttcat cacgcccgtg ataaagctgc ttatccggcg gctcgacgta
    26341 agtcgtattc tcagcgaagc aacccgcggc tttgcgcgcg ttaccttcat tccatccatc
    26401 tgcaatggtt tgcattaatc gttcaaattc tggttttgtc attggattgg gttagcaacg
    26461 cattgactct actgaaggat cacggtcgct tcaatctcca ccagcatttc aggattgatc
    26521 aatgctttca cctccaccat ggttgaagca ggtttgatgt ttttaaaaaa ttcaccgtgt
    26581 gctctgccga tttcttccca gcgagaaatg tcagtcgtga agatgcgcgt tcgcacaaca
    26641 tctgagagcg ccgcacctgc agaaatcaaa gctttttcga tcttggcgat gatatatctg
    26701 gtctgttcgt agggatcatc cggaccgaca agatttccct cttcatcgag cgcggtagtc
    26761 ccagctactt cgatcacatt gccgattctc gcggcgcgtg aatagccaac gatttcttcc
    26821 cactttaccc cagatgagat gttaagtcgc ttgctcattc ttactcctct ccatacgaaa
    26881 gctccaaagc gattgccgca ccctacaccg aatgtcacca gtggaccaga tcggtcgcct
    26941 ggatgacatt atctgcctca gatgtcactt cgccaatgaa gtgatgtact ccaaatcgag
    27001 ttcacctttc gcaccccagc cttttgtacc ctcaggaatt ttcggtcgga acttttcata
    27061 taaactgtaa gctgtctttt cgagttgttt tggtggatac gccttggcta acttttccat
    27121 ggccgcgcgg gcagcctcca aatcctcacc gaatttttgt ttcaaatatt tttcgacact
    27181 ttccgcgtcg atttcctccc ctttgatcgc ggcgcgcaat ccctgcttgg ttttgattgc
    27241 tggcacacca cgccccagca tttcgacgat ttcggttttt actggctttt ccttttttgt
    27301 ttctttgtct ttttcttctt cggatttctc ctcataaatc cccaatctcc tcactttcga
    27361 ttgtgcattg agccccgcga ctgcttttcc gaaggtgagt gcttcgtctt ttttgtatcc
    27421 cattctttct gcgacaattg ctgtccagag cgttagtacc ggagcacgat taattttgat
    27481 ttttttgttg ggcatttgat ctactcctct actatatata tagacacgat gcaaagaagt
    27541 aatgtgttat tcacaatata cactcatttc aatcatcctt ccaacgctat ccttaccgcc
    27601 tcttccaatg accattcgct ccccgtttcc cacgcggact gaaactccgc ctcagtcaac
    27661 tgtgcacgaa ttgcggtcag tgtttgcgcg tggaagtgca tgatatctgg ctccatcacc
    27721 gcattgagcg attttaatgc agatgcgact gcacccaaaa gttgcgcagc gaattgtgga
    27781 ttgcctgcgt gtaataagag gccggctact ccaatcaaac tggaagtctg tggcaattgt
    27841 tcgcctcttt cctggcgaag gcgcaggctt tgcaaaatgt gttcgcgtgc ctccggcata
    27901 tcttctgata attcacccag ccccagccca agtaa
//
LOCUS       DASTJP010000586         9475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_890206, whole genome shotgun sequence.
ACCESSION   DASTJP010000586 DASTJP010000000
VERSION     DASTJP010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9475
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890206"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1967)
                     /locus_tag="VFQ23_17295"
     CDS             complement(<1..1967)
                     /locus_tag="VFQ23_17295"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HET9908408.1"
                     /translation="MQELNARQVKIIDSFWSPRLAVNAQKAIFHQWKQLEATRCIDNF
                     RLVTGEKEGFREGWFFADSDAYKWLDAASRIYALDPQPKLAALMDSFIALLGRAQLPD
                     GYLFTYNQLHFPNQRWVNLQIEHELYCHGHLIEAGVSHFEATDRRDLLDICIKAADLL
                     VRDFLNASNDKTCGHEEIELALMRLYRVTGTGEYLELARQFVERRGRTPFYGIHWWRE
                     RNDFMKRNAYVNQLKQEYITKHPEHKSFRLPGGNYAKKPSYSKERYRINGFLGLYTQQ
                     HAPIRKQTSPVGHAVRFGYLETAIAMLVREEQNLLPQRTQSTQRNKPEGFSLETFVPF
                     VFKKESSNETLLSALEQAWERMVTKRMYITGGLGSAPEIEGFGGDYDLDPEYAYNETC
                     ASLASLFWNWEMALLTKNARYSDLFEWQLYNATNVGMGQSGSTYLYNNPLSVHGGVTR
                     QGWYVVPCCPSNLSRTFADLGKYIYSFDENNIWIHQYFSNETTINNVKIKIESELPWN
                     GKVRIHVRPESQKEFKIHLRQPSWNISSQQNGNTASAYDPRIADYEVIERVWSPDGET
                     LEFNFDMAIKLHRAHPKVKGHEGKVAITRGPLVYCLESVDNPDIDIFTAQLDPASIRD
                     EFFPDLFGGCVVIHASTMDGRPLRFIPYFLWA"
     gene            2074..2553
                     /locus_tag="VFQ23_17300"
     CDS             2074..2553
                     /locus_tag="VFQ23_17300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET9908409.1"
                     /translation="MNIPSAERCISCATVSGIFVSPGGVIYENSKWIVALRANPVRFP
                     CLPLIILKRHCEAVAELDDEESSSLGQIIQLTARAVDEVLRPAKIHFGIYAEEVKHVH
                     VHVFPRMPNMPAGNIPNLWVEQWMNLRHALGLKKSYSNQVVAEYARNLQRAYLNLAQ"
     gene            2568..3548
                     /locus_tag="VFQ23_17305"
     CDS             2568..3548
                     /locus_tag="VFQ23_17305"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9908410.1"
                     /translation="MKICIVGGTGNISQAIVRLLLQHGHEVVCFNRGQKTNARSDVRL
                     IQGDRHNREDFEQTMQAEKFDAAIDMICFNAEDAASSVRAFRGVGWFVQTSTTCTYGI
                     QYDYFPADESHPLRPNTDYGRNKVAADDVYLEAFHRERFPAVIIKPSTTYGPVQGMLR
                     QICWDFSWIDRVKKGKPILICGDGFALHQHMHVDDIAQAFVGVIGKENTIGQTYNAVN
                     RGYITWVDYHRLAGQILGKEVELVGVPFEDLKKLNVPNSGILEDEFAYNAYYSSEKLS
                     RDVPEFHPMITLEQGMTQVFEVMEREGRIPNSDELDWEDDIIERQRRILK"
     gene            3715..4614
                     /locus_tag="VFQ23_17310"
     CDS             3715..4614
                     /locus_tag="VFQ23_17310"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9908411.1"
                     /translation="MNPNPYLQKLGLSENDRAVIIHTDDIGMCHASVQAFKDLWAFGT
                     ITSGAVMVPCAWFPAVAQMCRENPEIDMGVHATLNAEWESYRWGPVSTRDQSSGLMDA
                     DGYFHQWHPGVYENAKPEAVEVEVNAQIERALAAGIDVTHVDSHMGTIMSPLFIQSYI
                     QAAATRNLPNMLPRLDAKGMTFMIAGEDERKNYKPIMKQSLESGVPMLEGLLGMPLNE
                     PDSGRQMEMAKQLLGDLPVGITHFILHPSIDSAELRSIAPDWESRVANYDVFMSDELK
                     HVLDSEDIKLIGYRQIRDAMRGG"
     gene            4611..5582
                     /locus_tag="VFQ23_17315"
     CDS             4611..5582
                     /locus_tag="VFQ23_17315"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET9908412.1"
                     /translation="MRYFLGIDVGSSKTHALIVDETGRCVGFGKSSGGNHQSVGYDGL
                     TNVLTESFAQARKMSRVDIADIAGAGFGVAGYDFLSDRSGHLQAIASLRLSCLVEVVN
                     DGMNGLLAGATRGIGVNVTAGSSNNCRGRGRNGQEGRIVGNGIRFGEFGGALEIVERG
                     LQMVSYAWIKRIPPTRLTKIYAEAVGAKDELDLLEGISNSRYHPYSFMTIEIIKAAQE
                     GDTAALDVLQWAGEELGWLAVSVARQIEMAEDEVEVIQSGSVFEAGELITTPMSEIVL
                     AHCPKAKLIRLEGPPVVGAVILGMEQARFDGYAIRDKMVRTAKEIVR"
     gene            5687..8062
                     /locus_tag="VFQ23_17320"
     CDS             5687..8062
                     /locus_tag="VFQ23_17320"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HET9908413.1"
                     /translation="MEKTPEQSRWWDWPAILLLCVLLQTVAARLIATDWVPDLPLIQL
                     FTTMGTAIGLALGYSQFPRRTARWISFFYMIILLPLQWTLVIDQEAALEEQLLSVGGR
                     LLFSISEFFSRRPVEDPLFFIAAMSIAYWAISASAAFTLLRQQNFLRVVAPSAIGMLI
                     IQNYDNVAAGRLWFLAFFVFVSLFLLGRINFLQDQKQWREKRVFLSPENSVDLTSGMA
                     IAAGLIILIAWTVPISFTRIDSARQTWNRLVKPWTDFAERMENAVSALESPSGGRPGE
                     FYGTELELGQGFPLSDTVMFQVQVPDLSSDERPPRYYWRGRVYDYFTRDQWYTTGTSR
                     QEYSPLNPGPAIPETTEGNLSRFVIGTGEERFSLLYAPQQLAWVSRGGSYLAAPAGEN
                     TQTRDVFSWNASPTLMPGETYQVDSMLKNPNITELREAGTDYPEWVTERYLQLPEDFS
                     PRIIELAQQITAEAETPYDKAGAITRYLRDNIEYSETIPAPPRNADLLEWILFEHKQG
                     YCVYYATSQILMLRSLGIPARMAVGFAQGEGISVGDNLAPEEGLAPGIFTVRKKNAHA
                     WPEVYFPGIGWVEFEPTGNQDPLNRPVAPQDPSDANNFNPVGNLPLGEGPDLPSPDRG
                     ELGDDLSNGANQSISPLLYLIPLIFAFAALAYYLGRRYSIPTRVPVFLRSSIERSGAA
                     APAWVVNWERWVSLSPIEKAFESINFGLRSLHEPAAVHATPVERADKLSMILPHLKNP
                     IKVLLDEHQTSLYTSRVGDAAEAQRAAFQLRMQVILSRIRHFWTGSYITKT"
     gene            8099..>9475
                     /locus_tag="VFQ23_17325"
     CDS             8099..>9475
                     /locus_tag="VFQ23_17325"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908414.1"
                     /translation="MATAKTKSISINDQLIAITQQGAKLRSSLDEKRDVVAVEGLKSL
                     ENSLERISEILIPFEQRHSHLQALAGIGQVVNSTLEIDEVLQIVMDTIVRLTEAERGF
                     LMLRDERGEMVNRIARNWEQESINQSEFAISRSIIQRVLNVGEAILTTNAREDPRFGG
                     QESIIAFNLRSILCVPLIIKNDIIGVIYTDNRIRSGIFSESERDLLIAFANQASVAIE
                     NARLFSSLKRTLEEVTELKNLMDDVFASIVSGVITADVQDQITLCNRAAASILGQTSA
                     EIVGRKLEEIIPTFANDIQPHMDSVRHSEQPIVGLEFSQSLPQRGNVDWRLNLSPLKD
                     AGQKTQGVAIVLDDLTERKRLEAQRRLFERMVSPAVIDQLDPNSLQLGGKRTDITVLF
                     ADVRGFTSFSETVAPERLVSILNRYLAAMAEAVLAQEGTIDKFMGDAIMAWFNAPVPQ
                     KDHTLRAVK"
BASE COUNT         2336 a         2370 c         2445 g         2324 t
ORIGIN      
        1 gcccatagga aatagggaat aaatctcaac ggcctaccat ccatcgtgct ggcatgaatc
       61 accacacacc cgccgaacaa atcagggaaa aattcatctc gtattgatgc agggtcgagt
      121 tgcgcagtga aaatatctat atccggatta tccacacttt caaggcaata gaccaaaggt
      181 ccacgtgtga tggcaacctt accttcatgc cctttgactt ttgggtgcgc gcggtgcagt
      241 ttgatggcca tgtcaaaatt gaattctaat gtttcgccat ccggcgacca gacgcgttca
      301 ataacttcat aatccgctat acgtggatca taggctgaag cggtatttcc attttgctga
      361 gatgaaatat tccatgaagg ctgacgcaag tgaatcttga attctttctg tgactccggt
      421 ctgacgtgga tacgcacctt cccgttccac ggcagttcag attcgatttt gattttcaca
      481 ttattgatag ttgtttcatt gctgaaatat tggtggatcc aaatattgtt ttcatcaaac
      541 gagtaaatat atttccccag gtcagcaaac gtgcgcgaga gattggatgg acagcaagga
      601 acaacatacc agccctggcg cgtcacaccg ccatgcacac tcaatggatt gttataaagg
      661 taagtacttc cactctgccc cattccgacg ttcgtggcgt tgtagagctg ccattcgaac
      721 aggtcactat agcgcgcgtt ctttgtaaga agcgccatct cccaattcca aaacaggctt
      781 gccagtgaag cacacgtttc attgtaggca tactccgggt caaggtcgta atcgccaccg
      841 aagccttcga tttcaggggc agagccgagc ccgcctgtga tgtacatgcg tttggtcacc
      901 atccgttccc aggcttgctc aagtgcggat aatagagtct catttgaaga ctctttttta
      961 aacacaaagg gcacgaaggt ctccaaggaa aaaccttcag gtttgtttct ttgtgtactt
     1021 tgtgtccttt gtggtaaaag gttttgttct tcccgtacta acatcgcgat cgcagtttcg
     1081 agatacccga aacgcacagc atgacccacc ggtgaagtct gtttgcggat gggcgcgtgc
     1141 tgttgagtat aaagaccaag aaacccattg atgcggtaac gctctttgct ataggatggc
     1201 tttttcgcgt aattgccccc cggcaaacga aatgacttgt gttccggatg cttggtgatg
     1261 tactcctgct tcaattgatt gacataagca tttcgcttca taaagtcatt gcgttcccgc
     1321 caccagtgaa ttccgtagaa gggagtccgc ccgcggcgct cgacgaattg ccgcgcaagt
     1381 tccagatatt ctcccgttcc agtcactcga tacaaccgca tcagtgcaag ttcaatctct
     1441 tcgtgcccac aggttttgtc attggaagca ttcaggaaat cacgcacaag caggtcagcc
     1501 gccttgatac agatatcgag caagtctcgg cggtccgtgg cttcaaagtg cgacacccct
     1561 gcttcgatga ggtgcccatg acaatacagt tcatgctcga tttgcaagtt gacccaacgc
     1621 tgattcggga agtgcaattg gttatatgta aagagatagc catcaggcag ctgcgcacgc
     1681 cccagaagtg caataaacga atccataagg gcagcaagtt tgggttgtgg gtcaagcgcg
     1741 taaatccgtg aagcggcatc cagccatttg taggcatccg agtcggcaaa gaaccagcct
     1801 tcgcggaatc cttccttctc gccagttacg aggcggaagt tatctatgca acgtgtcgct
     1861 tcgagttgtt tccattgatg aaagatcgct ttctgcgcat tgacggcgag gcgcggcgac
     1921 caaaaggaat ctatgatttt gacctggcgg gcgttaagtt cctgcataat tgttcctgac
     1981 cgaattatag gttaaatttg cgactctttc ttgttgacgc aataaggaat tctttgtaca
     2041 ctcccctgtc ggccaatcac aaggatgaac gagatgaaca taccctcagc agaacgctgc
     2101 atttcctgtg ccacagtttc tggtatcttc gtgagtcccg gaggagtgat ctacgagaat
     2161 tcaaaatgga tcgttgcact gagggcaaat ccggtgaggt ttccctgtct gcctctcatc
     2221 atcctaaagc ggcattgtga agctgtggca gaactggatg atgaagaaag ctcttccctc
     2281 ggacaaatca tccagctcac tgccagagcc gtagacgagg tgctgcgacc agcgaaaata
     2341 cacttcggaa tctacgccga ggaggtaaaa catgtgcacg ttcacgtttt ccctcgtatg
     2401 ccaaatatgc cagccgggaa cattccaaat ctttgggtcg agcaatggat gaatctcagg
     2461 catgcgctcg ggttaaaaaa atcttattcc aatcaagtcg ttgctgaata tgccaggaac
     2521 ttacaaagag catacttaaa tttggcacag taactccagg agaaaccatg aaaatctgca
     2581 ttgttggagg aaccggaaat atcagccagg ccattgttcg gctgttgctg cagcatggac
     2641 atgaggtcgt ctgtttcaac cgtgggcaga agacaaacgc gcgatccgat gttcgtctga
     2701 ttcaaggcga caggcacaat cgggaagact ttgagcaaac gatgcaggct gagaagttcg
     2761 acgcagccat tgacatgatc tgcttcaacg cagaagatgc cgcttcaagt gttcgtgcat
     2821 ttcgcggcgt gggttggttt gtgcaaacct caaccacctg cacctacggc atccaatacg
     2881 actacttccc cgctgatgag agccacccct tacgtccgaa cacggattat ggacgcaaca
     2941 aagtcgcggc ggatgatgtt tatctcgaag cctttcatcg cgagagattt ccagctgtca
     3001 tcatcaaacc ttccacgact tatgggccag tgcagggcat gttgagacag atctgctggg
     3061 acttctcttg gatcgaccgt gtcaaaaaag gcaaaccgat cctcatctgt ggagacgggt
     3121 ttgcattgca tcaacacatg catgtggatg atattgcgca agcctttgta ggtgtgatcg
     3181 ggaaggaaaa cacgatcggg caaacataca acgccgtcaa tcgtggctat atcacctggg
     3241 tggattatca tcgcctggca gggcaaatcc ttggtaaaga agtggaactt gtgggtgtac
     3301 cgtttgagga tttaaagaaa ctcaacgttc caaattctgg cattctggaa gatgagtttg
     3361 catacaacgc atattattcc tccgaaaaac tttcacgaga tgtacctgag ttccatccaa
     3421 tgattacgtt ggaacaaggc atgactcaag tctttgaggt gatggaacgc gagggaagaa
     3481 ttccgaattc tgatgagtta gattgggaag atgacattat tgaaagacaa agaagaatac
     3541 tgaaatagtc ggtggtcgag tagcctgagg cactagccga aggcatatcg agaccatcac
     3601 tgacaaaatt actttgatta cttgctggtc tcgacacctg cacttgcatg ccggtgcaag
     3661 tgtacggcga ttagtatcgc ctactcgacc aacagacttt tgaaaggata acaaatgaat
     3721 ccaaacccat atctccaaaa gttaggcctt tccgaaaacg atagagctgt catcatccat
     3781 accgatgaca ttggcatgtg ccatgcctcc gtccaggcat tcaaggactt gtgggcattt
     3841 ggcacgatca catcgggcgc cgtgatggtg ccctgcgcat ggttcccggc tgttgcccag
     3901 atgtgcagag aaaaccctga aattgacatg ggcgtgcatg ccaccttgaa cgcagagtgg
     3961 gaaagctatc gctggggacc cgtgtccacg cgggaccagt catccggtct gatggacgcg
     4021 gacggttatt tccatcagtg gcaccctggt gtttatgaga atgcaaaacc cgaagctgtt
     4081 gaagtggaag tgaatgcaca aatcgaacga gcgctggcag ctggtatcga tgtgacccat
     4141 gtcgattcgc atatgggcac gatcatgagt ccgttgttca tccaatctta catccaggca
     4201 gcagcgacga gaaatctgcc gaacatgttg ccacgcctcg acgccaaagg catgaccttt
     4261 atgatcgcgg gcgaagatga gagaaagaac tacaaaccca taatgaaaca gtccctggaa
     4321 tctggagttc caatgctcga agggctgctt gggatgccgc tcaacgagcc ggattcgggt
     4381 cgccaaatgg aaatggcaaa acaacttctg ggtgatttgc cggtgggtat tacgcatttt
     4441 atattgcacc cttcgattga tagcgctgaa ttgcgttcca tcgctccgga ctgggaaagc
     4501 cgggtcgcca actacgatgt ctttatgagt gatgaattga aacacgtgct tgatagtgaa
     4561 gacattaagc taattgggta tcgccaaatc cgtgatgcaa tgaggggtgg atgaggtatt
     4621 ttctggggat cgacgttggc tcgtcgaaaa cgcacgcgct gattgtggat gaaacgggac
     4681 gttgcgttgg gtttggaaag tcttctggcg gaaaccatca aagcgttggt tatgatgggc
     4741 tcacgaatgt cttaaccgag tcgttcgcac aagcccgaaa aatgtcgcgg gtagatatcg
     4801 ctgatatagc gggagcaggg ttcggcgtgg cgggatatga tttcctgtct gatcggagtg
     4861 gtcatttgca ggctatcgca tcgctcagac tatcttgcct tgttgaagtt gtgaatgacg
     4921 gcatgaatgg tttgcttgca ggtgcaacac gcgggattgg tgtcaatgtc accgcagggt
     4981 catccaataa ttgccgtggg cgcggaagaa acggacagga gggtcgtatt gtcggcaatg
     5041 gaatacggtt cggtgaattc ggcggggcgt tagagatcgt agaacgtggt ttacagatgg
     5101 tgagctatgc atggatcaaa cgcatcccac ctacaaggtt gacaaaaata tatgccgaag
     5161 cggtgggtgc aaaagacgaa ttggatttac tcgaaggtat ttcgaattct cgatatcatc
     5221 catattcttt tatgacgatt gagattatca aagcggcgca ggaaggggat actgctgctc
     5281 ttgatgtgtt gcaatgggct ggcgaagaat tggggtggct ggcggtctcg gtagcgcggc
     5341 agatcgaaat ggccgaagat gaagtggaag ttattcaatc cggcagtgtt tttgaagcgg
     5401 gtgagctgat tactacaccc atgagcgaaa ttgtgcttgc tcattgtcca aaggcaaaat
     5461 tgatacggct ggagggccct cccgtcgttg gggctgtgat cctcggtatg gaacaggccc
     5521 ggtttgacgg atacgccatc cgtgacaaga tggttcgaac cgcaaaggaa attgtgaggt
     5581 agtttttctc tggggagtag aagagtttat tcttttagcg aacgctaatg aaaaaacccc
     5641 tctacagtat ttgacgaacc tccctcttaa ttgtagactc atcactatgg aaaagacccc
     5701 tgagcaatcc cgctggtggg attggcccgc tatcctcctg ctttgtgtgc tattgcaaac
     5761 tgttgcagcg cgtctgattg ccaccgattg ggtgccagat ctgcctctca tccagctatt
     5821 taccaccatg gggactgcca ttgggctcgc cctcggatac agccagtttc cgcgcagaac
     5881 cgcccgctgg atcagttttt tctacatgat cattttgctg ccattgcagt ggaccctggt
     5941 gattgaccag gaggctgcac tggaggagca gttgctaagt gttggcggtc gccttctctt
     6001 ttcgatctcc gaattcttct cacgccgccc tgttgaggat cccctcttct tcatcgcggc
     6061 catgtctatc gcctattggg caatcagcgc ctcggcagcg tttacgctct tacgtcagca
     6121 gaatttcctg cgggttgtag cgccctctgc cattggtatg ctgatcatcc aaaattatga
     6181 taatgtggcg gccggccgat tgtggtttct ggcctttttt gtctttgttt ccctttttct
     6241 tttagggcgt attaacttcc tgcaggatca aaaacagtgg agagagaagc gcgtcttcct
     6301 ctcccccgaa aacagcgttg atctgaccag tgggatggca atcgctgccg gattgatcat
     6361 cctgatcgcc tggacggtgc ccatttcctt cacacggatt gactctgcac ggcagacctg
     6421 gaaccgtctc gtcaaaccat ggaccgattt cgcagaacgg atggaaaacg cggtcagcgc
     6481 gcttgaatcc cccagtggtg gacgacccgg tgaattttac ggaacagaac ttgaacttgg
     6541 gcaaggtttt ccactttcgg ataccgttat gttccaggtt caggtccccg atctgtcttc
     6601 tgacgagaga cccccccgtt actactggcg gggacgcgtc tatgattatt tcaccagaga
     6661 tcaatggtat accacgggca ccagccgcca ggagtattct ccgctgaacc ccgggcctgc
     6721 cattccagag accacagaag gaaacctctc tcgcttcgtg atcggaacgg gtgaagaacg
     6781 gttttcgctc ctgtatgccc cgcaacaact tgcatgggtt agccgcggcg ggagttacct
     6841 ggcggcgcct gccggggaaa atactcagac tcgcgacgtt ttctcgtgga atgcatcgcc
     6901 cacgctaatg ccgggtgaga cgtaccaggt cgattccatg ttgaaaaatc cgaacatcac
     6961 agaactccgt gaggccggaa cagattatcc tgagtgggtc acagaacgat atttacaact
     7021 acccgaagat ttttcgccgc gtattatcga actggctcaa cagatcacgg ccgaagccga
     7081 gactccctac gacaaagccg gcgccatcac ccgctacctg cgcgacaaca ttgaatattc
     7141 tgaaaccatt cccgcgccgc cgcgtaatgc cgatttgctc gagtggatct tatttgaaca
     7201 caaacaaggc tattgtgttt attacgctac ctcacaaatc ctgatgcttc gttcgctcgg
     7261 gattccggca cggatggcag tgggctttgc gcagggtgaa gggattagcg ttggagataa
     7321 cctggcaccc gaggaaggtc tggctcccgg catcttcacc gtacgcaaga aaaacgcaca
     7381 cgcctggcct gaggtttact tcccgggtat tggctgggtg gaatttgaac ccaccggaaa
     7441 ccaggatcct ctcaaccggc cggtggcacc acaggatcca tcagatgcga acaacttcaa
     7501 cccggtcggc aatcttccgc ttggggaagg tcctgatctc ccatcaccgg accggggcga
     7561 attaggcgat gacctctcca acggggcgaa tcagtccatt tctcccctgc tgtacctgat
     7621 cccattaatt ttcgccttcg cagccttagc atattacctc ggacgccgct attccattcc
     7681 gacacgcgtc ccggttttcc tgcggtccag catcgagcga agcggagcgg ccgcgccagc
     7741 ctgggtggtt aattgggaac gctgggtttc tctttccccc attgaaaaag cattcgaaag
     7801 catcaatttc ggtctgcgct ctctgcatga acctgcagcg gttcatgcga cacccgtgga
     7861 gcgtgccgac aaactttcga tgattttgcc ccatctgaag aatccgataa aagtactgct
     7921 ggacgaacac cagacatccc tttatacttc cagagtcgga gacgctgcag aggcacaacg
     7981 cgcggccttc caactcagaa tgcaggtcat tctttcaagg attcgtcatt tctggactgg
     8041 ttcatacatc acaaaaacat aaaccattct ttcccaacca ggggagagag gaaaaccaat
     8101 ggctactgcg aagacaaagt ccatctcgat caacgatcaa ctcattgcca ttacccagca
     8161 gggggcaaaa ctgcgcagtt cgctggatga aaagcgcgac gttgtagctg tggaagggct
     8221 caagagcctc gaaaattccc tggaacgaat cagcgaaatt cttatcccgt ttgaacagcg
     8281 acatagccac ttgcaggcac tggcgggcat tgggcaggtg gtcaactcca ccctggaaat
     8341 cgatgaagtt ctccaaatcg taatggatac catcgtccgc ctcaccgagg cagagcgtgg
     8401 ttttctgatg ctgcgcgatg aacgcggcga aatggtcaat cgcatcgccc gcaattggga
     8461 acaggaatcg atcaaccaga gtgaattcgc catcagccgc tcgatcatac agcgcgtcct
     8521 aaatgtcggc gaagccattc tcacaaccaa cgcgcgtgaa gacccgcgct tcggcggtca
     8581 ggaaagtatc atcgcgttca accttcgttc gattctttgt gtaccattga tcatcaagaa
     8641 tgacatcatc ggtgttatct atacagacaa tcgcatccgt tcaggaatat tttcagagtc
     8701 agaacgtgat ttgttgatcg cgtttgccaa tcaggcttcg gttgccattg agaatgcccg
     8761 tctgttttct tctctcaagc gcacgcttga agaagtgact gaattgaaga acctcatgga
     8821 tgacgtattc gcatcgatcg tctctggcgt aattacagcc gatgtgcagg atcagatcac
     8881 cctctgtaac cgagctgccg cctccattct gggacaaact tctgctgaaa ttgtcggtcg
     8941 gaaactggag gagattattc ctacgtttgc gaacgacatt cagccccata tggattcagt
     9001 ccgccatagt gaacagccta ttgttgggct cgaatttagc caaagcctgc ctcaacgcgg
     9061 caacgtggat tggcgattaa atctctcccc gctaaaagac gcgggacaaa aaacccaggg
     9121 cgtggcgatc gtgctcgatg acctgacaga gcgcaaaaga ttggaagctc agcgccgact
     9181 ctttgaacgg atggtctccc cggcggtgat cgatcaactg gaccccaaca gcttacagct
     9241 tggcggcaag cggaccgata ttactgtgtt gttcgcggat gtacgcggct ttacttcgtt
     9301 cagtgaaacc gtcgcaccgg aacggctggt ttcgattttg aaccgttatc tggcagcgat
     9361 ggccgaggcc gtgctcgcac aggaaggtac gattgataag ttcatgggcg acgcgatcat
     9421 ggcatggttc aatgcgcccg tgccacaaaa ggatcatacg ctgcgggctg tgaaa
//
LOCUS       DASTJP010000587        13050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1313225, whole genome shotgun sequence.
ACCESSION   DASTJP010000587 DASTJP010000000
VERSION     DASTJP010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13050
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313225"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..3505
                     /locus_tag="VFQ23_17330"
     CDS             263..3505
                     /locus_tag="VFQ23_17330"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HET9908415.1"
                     /translation="MRKTINSSVPHKLLTALVILSMVMVALPATTAFAAGSTISVTTT
                     DDEYDGGPLNVCSLREAIALANHPTALTLNDCSRSVAGPDDEIVLQSGQTYLLDRTGA
                     GGTSEGDLDIGDNSGLSGNLTISTTGSAPATINGNGTDRVFDVGAAGNPGLTLNNIIV
                     TNGNTTGLLSSTTGGGISFAGTGTLSLINSTVSNNVSASSTGCGGGIYNNSAATVVIT
                     DSTISGNSCPNGNADGAGMLKGGGGTLTVTGSTFSGNTVGENGGGMHIGGSAATTVNI
                     TNSTFANNTAGNRGGGLQVGNAAAVVTIDFTTFSGNSASESTLTPPGLDGGGALQVTA
                     GTVTVTRSILANSTSTNPGTPEDCDVSTSVTTVSVSDSIIETNVDSADSDCGPSGVLN
                     VDPQLGALANNGGPTQTMGISLSSPAYNAADSCGSVVVDQRGATRPQVGACDLGAFES
                     GNTTAPVVSNVTRATTNPTNDPSVDFTVTFSLPVTGVDATDFQLVTTGVTGASITGVS
                     GSGTSYTVTVNTGSGDGTIQLNVLDNGSIASGGDGLDGGFTNGEVYDVDKTLPTVTIS
                     RADPNILSSATVDFGVSFSEAVTGVNTADFNLAVSSGITGASISGISGSGATYTVTVN
                     TGTGDGTIGLNLNGAGTGIADAAGNAIGGAVTGEVYTILRSAAAVDVDIAGTNVGDGI
                     IIPFNNGQRVGFAVDNGPAKVYSTNGFDIVAALRVIWREPGLRTSYSEMMGLPVEQLS
                     SEYWFPWYNNRDVASMDQGFRIANVDSSDTTVKVMLGTTELDSFTLAAGASVRTAYDV
                     NSGPIRIYSVEGNKILAALRVIWKEPGPRYSYSEMMGLPVEQLSTEYWYPWYNNLDIA
                     SMDQGFRIASVNGIGDNTVEVWAAGVLKQTFSLAQGASVRVGYEVNDGPVQVRCTTCS
                     GAEKIITSLRVIWKEPGFRATYSEMMGMPLEQLSTTYWFPWYNNADVNSMDQGFRIAI
                     VNGSGENTVQVFVAGALQESITLIENESVRVGYNANNGPAKIVCTTCTLDEKIVTSLR
                     VIWKEPGVRTSYSEMMGLPNEALSNEYWFPWYNNLFQTSIMDQGFRISVP"
     gene            3641..4471
                     /locus_tag="VFQ23_17335"
     CDS             3641..4471
                     /locus_tag="VFQ23_17335"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908416.1"
                     /translation="MKPHPPPVATPPAMFASFWRNRHLIKQMTRREIAARYRGSVIGI
                     AWSFITPLLMLVVYTFVFSVVFKARWGSSTDESRTDFAITLFAGMIVFGIFSEMVNRA
                     PWQIILNTNYVKKIVFPLEILSWVSLGSVLFHSLVSLVVLLLVQLIVNASLPWTVIFL
                     PLVLLPLILASLGVAWFLAALGVYVRDIGQFTTVFTTLLMFVSAVFYPVSSLPEAYQA
                     WLRLNPLVLVITESRKVLIFGTPPDWLWLGIALVFGLVIAFTGFWWFQKSRVGFADVI
                     "
     gene            4461..5867
                     /locus_tag="VFQ23_17340"
     CDS             4461..5867
                     /locus_tag="VFQ23_17340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908417.1"
                     /translation="MSSSAIVIKVEHLSKCYNIYNRPQDRLKQAIIPRLQRLVSRPPI
                     RYFHEFWALRDVSFEVHKGETIGIIGRNGSGKSTLLQLVCGTLSPTSGTLTTEGRIAA
                     LLELGSGFNPEFTGRENVYLNAAILGLSNEEINERFDAIASFANIDDFMEQPTKIYSS
                     GMMLRLAFAVQAMVEPDILIVDEALAVGDEKFQRKCFARLEELKSKGTSILFVSHSSS
                     QILELCDRALLLESGEKLMLGQPSRVINAYQKLIYSPISQQEDLVKQFRDADQTVIGT
                     DLAPMRSSGDEDSSLGQISIGAAPLQEALESNFFDQTLIPASTVIYPIQGAEINSLRI
                     LNAHGQSVNLLSFGQIYRFELSGTFLTERKNVYIGIHIRTISGLVLTGQRYPEEGQYI
                     EKVHAGEHFALTFGFKMNLSSDTYFSGCGIWSLDEPQCLHRIIDAVMFRILPEPKVKF
                     LGYLDVSSMEPILKTWKN"
     gene            5965..8325
                     /locus_tag="VFQ23_17345"
     CDS             5965..8325
                     /locus_tag="VFQ23_17345"
                     /inference="COORDINATES: protein motif:HMM:NF027586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF707 domain-containing protein"
                     /protein_id="HET9908418.1"
                     /translation="MKDKYVLVSFCNIPVYTTSPLAVLQISEKQGNLVSYAPVALGYA
                     GPLKTATGLTSANGKIFVLFTTRDGKTYVAALKERDLSPLFHCELPEIKDGHSILALR
                     DCLYVVSTGTDEIICYDIKDSRVENPRVVWHASNTKQDTHHVNSIIEYNGDVLISAFG
                     RKKGELWTTASNGYIFNITSNSLITDGIHHPHSLSIKNDKIYYSDSNRNAFCMVGDPE
                     PIFDLNGYARGITWLSDDIVCLAISIGRKVSKSTGRIANPADPGEPAGECGLVIGKVS
                     NKEVLEKIDLSWFGPEIYDTLILPETAADLLSLSNTAQRSERQTIDIFSRELNDLRQQ
                     TTRQIQELSSQLAGREQRLQEILNSRAWKLVQYIRGSYEVIAPFGSTRDTLIQLVFRT
                     ALIGRQQGLKAILRQAYNWAWRSTNGWARLSSRSVRSRRSYISIPGLSQALREKAKKI
                     ESSGFFDHEWYLQDNPDVRISRINPVEHYLTMGLSIEKNPNPLFNTSIYVKSADENVE
                     PEDALLHFVESDRLFAPGAYRNAEILLTAQQNYLKELDMEYQDRRSIPKRFAVFFQCG
                     SGSVHKEWLTENSKDWDLLINHYDETYLNKISCDVEFQQTGKKAGTKSTAFHMLLERW
                     PQLLEPYDYVLLLDDDILLEEKDISRLFHIASEHNLHLAQASLSSNSNCAHQIFKNPG
                     KTGIRYVDGVEIMMPLLSKRAIDVGRHLFRQAISGWGVDFVLGKLLSAKGGAAIIDDV
                     IVWHTKPINVEEGAFYRMLHEAYIYPEIELTNLQRIYGVGRAFNHV"
     gene            8356..10218
                     /locus_tag="VFQ23_17350"
     CDS             8356..10218
                     /locus_tag="VFQ23_17350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9908419.1"
                     /translation="MAIAVATDLIPIHRPDSTFNQSPMAVALALVKNEGDIIAAWLSH
                     ICELFDLVYIADHQSTDGTREYLLDQAKSQNKIHLFSFEGTGYFQSEITNKLTQIAIE
                     ENSDVWLFPLDADEFLPIPSKLDFISHLNKFKSDQILLLNWQNHIPLYLQYDQEINFN
                     TPFLRPSQPGLYDKVAVKSGTIRKMNWTFTQGNHAVATEAGTLAGLDAKVRFSSLIHI
                     PIRSVDHFTLKCIQGNIATYQLANWRTRGPLHWHEMLTSSLRSGSINPDLIRGFAQSY
                     GSMNNPMPVSVSVYDLIDAGWNVNTLNIAHENVVEGSFSRRKKFIQLATEILEATQEI
                     SDLQRFLQLSKDEQKSAMDASSWQKGSIPNSIKFNKLPDFDAKDLHPESSESELLQVF
                     VSSSFTPREIPMPSTWESHVPFLYCLLNFFEPRRFVELGTHHGNCFFAACQIAKQPNR
                     SIECIAIDTWAGDEHTGSYGNKIFDEFKYILRRDFPQGKYIRKFFNDAARQFEQGSID
                     LLHIDGLHTYEAVAEDFATWLPMLSNRGIIMFHDTYERARGFGVWKLWEEVSRKYPSL
                     EFEHGHGLGVLLVGNNPDPHIKRLFEIVSKKENADFLRFFFSNIGGLSPMTIRK"
     gene            10325..11392
                     /locus_tag="VFQ23_17355"
     CDS             10325..11392
                     /locus_tag="VFQ23_17355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9908420.1"
                     /translation="MRIIALTRYERLGSSSRVRFYQYFSYLRSQGVKISNEPFFSDRY
                     IQDLYAGRSVSLKDLLVAYIKRLAALKQRSAVDLLWVEKELLPMFPAGLEALFRSSKI
                     PYVVDYDDAVFHRYELHPNRLVRRFLKHKIDAVMGHAALVIAGNQYLADRAVAAGAPN
                     VTILPSVVDVSQYLIRQKVTGPVCSIGWIGSPVTVPNLELIRDAINLLHREMDFRIEL
                     IGAGTANPFPTIPTTSLAWSEEGELSIGQKFDVGVMPLIDGPFERGKCGYKLVQYMAG
                     AIPVIASPVGVNRQMVEQGRNGYLADSTEDWLLALRELLSNRQKNLEMGLAGRHKAEQ
                     LYNLQVTAPKLLNLLSSAVKV"
     gene            11419..12564
                     /locus_tag="VFQ23_17360"
     CDS             11419..12564
                     /locus_tag="VFQ23_17360"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9908421.1"
                     /translation="MLKILLFANTDWYLYNFRLSLAKDLRAQGHEVILLSPPGPFQKL
                     LREHGFQLVSFPFSRQGINPFYEIWTLWQLIRTYRTVRPDIVHHFTIKSVIYGSLAAH
                     FLRIRGIINSITGLGHLFIDPGPVTRLLRVVAKWLYRLGLRNTQVIFENPEDRTIFIQ
                     NHLLKAANSHLILGTGVDVEKFSPVSKKNDIPVVLFSSRLLSTKGVLEYMEAVQILKQ
                     NGFKARFALAGATDPGNPASVTDEQIESWSRSGLVEWWGWRDDMPNTLAQTDIFCLPS
                     YREGVPNALIEACAAGIPIVTTDVPGCRDVVTHGVNGLLVPVRNGTALAGALETLLVN
                     PEMRQEMGKAGREAAIHKFSDTKVNAETLRVYKVIIPANQPGKIGAD"
     gene            12611..>13050
                     /locus_tag="VFQ23_17365"
     CDS             12611..>13050
                     /locus_tag="VFQ23_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908422.1"
                     /translation="MQKIKGSQRILLAYSLFVAIISLLFLRGMILSPSESGSAVFLGL
                     SIPRLVISVGVLITWIFYVAIAVKAARNHEWAEKALEYWFGGGPAGTVARWIAGISFG
                     LGWIGCFLPAYRVGAFGNYWISLRPIMIFIMIVSVATLTVSFIKR"
BASE COUNT         3324 a         3120 c         3190 g         3416 t
ORIGIN      
        1 caaataagtt accgggaata ggctacatcg tcgaaaagaa aaaaagctgg cagttgctta
       61 agcaactgcc agcttttttt cttttcgacg atgtagccta ttcccggtaa cttatttgta
      121 acatagtcgc ttgtaacttt acgcaaggta gtgggtcttg tcactgatta agcagcaatg
      181 ggtctcacct attgtgatag gtaacctgtt gataaatttt gttaagtagg ttcgaatcca
      241 aattccaagg aaaggaaaga taatgcgaaa aacaattaat tcttcagtac cacataagct
      301 tctaactgcc ctggtgattc tctccatggt aatggtagcg ctgccggcaa caacggcatt
      361 cgctgctgga agcaccatct cagtcaccac gaccgacgat gagtacgacg gagggcctct
      421 caatgtatgc tctctgcgcg aggcgattgc ccttgcaaat catcctaccg cccttaccct
      481 gaatgactgc tcacgtagcg tcgcgggacc tgatgatgag atcgtgctac agagcgggca
      541 aacctatttg cttgaccgca ctggcgcagg cggcacatcc gaaggcgatc tcgatattgg
      601 tgacaatagt ggtctcagcg gtaacctgac catctccacc actggttcgg cgcctgctac
      661 tattaatggc aatggcacgg accgcgtatt cgatgtcggc gctgcgggaa accctggcct
      721 gacattgaat aatatcattg tcacaaatgg taacacgact ggtctgttat cctctaccac
      781 aggtggtggc atcagcttcg ccgggaccgg cacactttcg ctgatcaact cgacagtctc
      841 aaacaatgtc tctgcaagca gcacgggatg cggcggcggt atctataaca actctgccgc
      901 tacagttgtg atcaccgaca gcacaatctc gggcaacagt tgccccaacg gaaatgcaga
      961 tggtgccggt atgctcaagg gcggcggtgg tacgctcacc gttactggca gcaccttttc
     1021 ggggaatact gtaggtgaaa acggtggcgg tatgcatatt ggcggctcag cagctaccac
     1081 agtaaacatt accaattcca cttttgcgaa caacaccgcc ggcaaccgag gcggaggctt
     1141 gcaggtaggg aatgcagctg ctgtcgtcac gattgacttc acaaccttct ctggcaatag
     1201 cgccagcgag tccactttga cacctcccgg cctcgatggc ggtggagcgc ttcaagttac
     1261 tgcgggaacg gtcacggtca cgagaagtat ccttgccaat tcaacatcaa cgaatccagg
     1321 aacgccagaa gattgcgatg tgtcaacctc cgtcacaacg gtttccgttt cggactcgat
     1381 tatcgaaact aatgttgaca gcgctgacag tgattgtggt cccagcggcg ttctcaacgt
     1441 agatcctcag cttggtgctt tggcgaataa cggcggaccc acacagacga tgggcatttc
     1501 gctcagtagc cctgcttata acgcggcgga tagctgcggt tcagtcgtcg ttgaccagcg
     1561 cggcgccact cgccctcagg tgggtgcctg tgaccttggt gccttcgaat caggaaatac
     1621 tactgcgcct gtggtatcga atgtgactcg cgcaacgaca aatcccacca atgaccccag
     1681 cgttgatttc acagttacct tctctctacc tgttaccggc gttgacgcaa ctgacttcca
     1741 gctcgttacc acaggcgtaa ccggagcgag catcaccggc gtgagtggtt ctggcacgag
     1801 ttacacagtt acagtcaata caggttcagg cgatggcacg atccagctca atgtcctgga
     1861 taatggttca attgcatccg gcggagatgg cctggatggc ggctttacca acggtgaagt
     1921 gtatgacgtt gataagacct taccaaccgt cacaatatcc cgcgcggatc ccaacatcct
     1981 aagctcagcc accgttgact tcggtgtctc attctccgaa gcagtcacgg gtgtgaatac
     2041 agctgacttc aatctggccg tctcctcagg catcactggc gccagcatca gcggaatcag
     2101 cggctcgggc gctacttata ccgtcaccgt gaatacgggc accggcgatg gcacgatcgg
     2161 tctcaatctt aatggtgctg gcaccggtat tgcagatgct gccggcaatg cgattggtgg
     2221 agccgtcacc ggcgaagtgt ataccattct aaggagcgcc gccgcagtgg atgttgatat
     2281 cgccggaacc aacgtcggcg atggcatcat catcccgttc aacaatggtc aacgcgtcgg
     2341 ctttgcggtg gataatggtc ctgccaaggt gtacagcacg aacggcttcg acatcgtcgc
     2401 agcgttgcgg gtgatctggc gggaaccggg tctgcgcacc agctactcgg agatgatggg
     2461 attgccagta gagcaactct cgtcggagta ctggttcccc tggtacaaca atcgagatgt
     2521 cgcttccatg gatcaaggct tccgcattgc caacgtggat agctccgaca ccaccgtcaa
     2581 ggtcatgctc ggcactaccg agttggactc cttcaccctt gccgctggtg ccagcgtccg
     2641 caccgcctat gatgtcaaca gtggacccat ccggatctac agcgtggaag gcaacaagat
     2701 cctggctgcc ctgcgcgtga tctggaaaga acccggtcca cgctacagct actccgagat
     2761 gatgggtcta cccgtcgaac aactctccac cgaatactgg tacccctggt acaacaacct
     2821 cgatatcgcc tccatggatc aaggcttccg cattgccagt gtcaacggca tcggcgacaa
     2881 cacggtcgaa gtctgggccg ccggggtctt gaaacagacc tttagcctgg ctcagggagc
     2941 gagtgtccga gtgggctatg aagtcaatga tggtccggtt caagtgcgct gcaccacctg
     3001 cagtggagcc gagaagatca tcacctctct gcgtgtcatc tggaaggaac ctggcttccg
     3061 ggccacctac tccgagatga tgggcatgcc actcgagcag ctctccacca cctactggtt
     3121 cccctggtat aacaatgccg atgtcaactc catggatcag gggttccgca tcgccatcgt
     3181 caatggcagt ggcgagaaca ccgtccaggt cttcgtggct ggagccttgc aggaaagcat
     3241 cacgctgatc gagaatgaga gtgtgcgtgt gggctacaat gccaacaacg gtcccgcaaa
     3301 gatcgtctgc accacctgta cgttggacga gaagatcgtc acctccctgc gcgtgatctg
     3361 gaaggagcca ggagtccgca ccagctactc cgagatgatg ggtctcccca acgaagccct
     3421 ctccaacgag tattggttcc cctggtacaa caatctcttt caaacctcca tcatggatca
     3481 gggttttaga atctcagtgc cttaacttta gtgacgggcg accgcttgag caggcggtcg
     3541 ccctacctta gcttgacatg ccattctctt cgtggcaaaa ttcaaatcct gaattttgcc
     3601 acgaagagat atttttttaa actctatcta tcgttccgtt atgaagcctc atcccccccc
     3661 tgtagccacc cctcccgcta tgttcgccag cttttggcgc aaccgccatc ttattaagca
     3721 aatgactcga cgggaaattg cagctcggta tcgaggctct gtaattggaa ttgcatggtc
     3781 cttcattacg cctttattga tgctggtcgt atatacgttc gtgttttctg tcgtctttaa
     3841 agcgcgctgg ggttcgagta ctgacgaaag ccgaacagat tttgccatta cgctttttgc
     3901 agggatgatt gtcttcggca tattttccga gatggtaaat cgcgcgccct ggcaaattat
     3961 cttgaacact aattatgtaa aaaaaatcgt gttccctttg gaaatcctgt cctgggtatc
     4021 tctgggatca gttctttttc acagcctggt aagtctcgtt gtactgctgc ttgtacagtt
     4081 gatcgtaaat gcatccctgc cctggacagt tatattttta ccgcttgtac ttttgccatt
     4141 gatcctggcc agtttggggg ttgcctggtt cttagcagcc ctgggcgtct atgtccgtga
     4201 cattgggcag tttacaacgg tctttaccac actcctgatg tttgtctccg ctgtgtttta
     4261 ccctgtttct tcgcttccgg aggcttatca agcctggcta aggctgaatc ctttagtgct
     4321 cgtcattaca gaaagtcgca aggtactgat cttcgggact ccgccagact ggttatggct
     4381 ggggatcgca ttagtatttg gattggtcat tgcatttact ggcttttggt ggtttcaaaa
     4441 atcacgcgtg ggatttgcag atgtcatcta gtgctatagt gattaaggta gagcatctaa
     4501 gcaaatgtta caacatctat aaccgcccac aagaccggct caaacaagcg atcatacccc
     4561 gcctgcaacg actggtgagc cgccctccta ttcgttactt ccatgagttc tgggcgttac
     4621 gggatgtttc tttcgaggtt cacaagggcg aaacaatcgg cattatcgga cgaaacggat
     4681 caggcaaatc cacgctcctt caattggtct gtgggacgct gagccctacc agcggcacgc
     4741 tgacaaccga gggacgaatc gcggccttac tggagcttgg ctccggcttt aacccggagt
     4801 ttactggtag agaaaacgtg tacttgaatg cggcgatcct tggactttcc aatgaagaaa
     4861 ttaacgagcg gttcgatgca atcgcaagtt ttgccaatat tgatgatttt atggaacaac
     4921 cgacaaaaat atattcgagc ggtatgatgc tgcgtttggc attcgctgtt caggcaatgg
     4981 tagaaccaga tatcttaatt gttgatgaag ccctggctgt aggagatgaa aaatttcagc
     5041 gtaaatgttt tgccagactc gaagaactga agagtaaagg cacgtcgatc ctttttgtat
     5101 ctcattcgag ttctcaaatc cttgagctat gtgaccgggc gttgcttctt gaatccggtg
     5161 aaaagctgat gcttgggcag ccttcaaggg tgatcaatgc gtatcaaaaa ctgatttact
     5221 ctcccatcag ccaacaagag gatctggtca aacaatttag ggatgcagat caaacggtaa
     5281 ttggtactga tttagctccc atgcgttctt cgggtgatga agattcttca ttgggtcaga
     5341 tatcgatcgg cgccgcgccc ctgcaggaag ctctggaatc aaattttttc gatcagaccc
     5401 ttatccccgc ttccacggtc atctatccta tacagggagc ggaaatcaat tcactgcgca
     5461 tcctgaatgc gcatggtcaa agtgtgaacc tgctgtcgtt cggacaaatc taccgatttg
     5521 agctttccgg aacgtttttg acggaacgga agaatgtcta tattgggatt catattcgga
     5581 cgatctcagg gctggtcctt acgggtcaaa gatatccgga agaagggcaa tacattgaga
     5641 aagtccatgc cggagagcat ttcgcactta cttttggatt caaaatgaac ctttcgtcag
     5701 atacctactt ttctggctgt ggcatttggt cattggatga accccagtgt ttacatcgaa
     5761 tcatcgatgc agttatgttt cgaatcctgc cagaaccgaa agtgaaattc cttgggtatc
     5821 tggatgtttc ctccatggag ccgatactta aaacctggaa aaactaattt atgtccaaat
     5881 tgaatgattt gatagaattc aagacgctag agatacaaat attggcagga aattcataca
     5941 caagagtaag tcgatgagca aagaatgaaa gataaatacg tacttgtttc cttttgtaat
     6001 atcccggtat acacaaccag tcctttggct gtgttacaaa tcagtgaaaa acaaggcaac
     6061 cttgtctctt acgcccccgt cgcgttggga tacgccggcc cccttaaaac agccaccggg
     6121 ctaacatctg caaatggtaa aatctttgtc ctttttacaa cacgagacgg taagacatat
     6181 gttgcagctt tgaaagaacg cgatctatcg cccttattcc attgtgagtt accggagatc
     6241 aaagatggtc attcaatttt ggcgttacga gactgtttat atgtagtctc gacggggacc
     6301 gatgagataa tttgttatga tatcaaggat agtagggtgg aaaatccgcg tgtcgtatgg
     6361 catgccagta acacaaaaca ggatactcac catgtcaatt cgatcatcga atacaacgga
     6421 gatgttctga tttcggcctt cggccggaag aaaggggaat tgtggacaac cgcttcaaac
     6481 ggttatattt ttaatatcac atccaattcg ctgatcacag atgggatcca tcatcctcat
     6541 tccctgtcga tcaaaaacga caaaatttac tacagcgatt cgaacagaaa tgctttttgc
     6601 atggtcggcg atccggaacc gatatttgac ctgaatggct atgcgcgtgg gattacatgg
     6661 ttgtcagatg atatcgtttg cctggcgatc agtatcgggc gaaaggtgtc caaaagcacc
     6721 gggcgaattg ccaacccagc agatccggga gagcctgcgg gggaatgtgg attggtcatc
     6781 ggaaaagtat ctaacaaaga agttctagaa aaaatagacc tgtcgtggtt cggcccggaa
     6841 atttacgata cgctaattct tccggagaca gcagcagatc tactttcact ctccaacact
     6901 gcccagaggt ccgagcggca gacaatcgac attttctcca gagaactaaa tgatctaagg
     6961 caacagacga cgcggcagat tcaggagctc tcctcacaat tggctggccg tgaacaaagg
     7021 ttgcaggaaa ttctcaacag tcgagcatgg aaactcgtgc agtatattcg cggttcttat
     7081 gaggttattg cgccgtttgg cagcacacgc gacacgttga tacagcttgt ttttcgtacc
     7141 gcacttatcg ggaggcagca aggtctcaaa gctatccttc gtcaggccta caattgggcc
     7201 tggagatcaa ccaatggatg ggcaagatta tcgtcgcgat cagtccgctc acgacgatcg
     7261 tatatttcga taccggggtt gtctcaagcc ttgcgtgaga aggcgaaaaa aatcgaaagt
     7321 tcagggtttt ttgatcacga atggtatctg caggataacc ccgatgttcg aatatcacgc
     7381 attaatcctg tagagcatta cttaacgatg ggattaagta ttgaaaaaaa tccaaatcca
     7441 cttttcaata catccatcta tgtcaaatcg gccgatgaaa atgtcgagcc ggaagacgcc
     7501 cttctgcatt ttgttgaaag tgatcggctc tttgccccgg gagcctatcg aaatgctgag
     7561 atcctgctta cggcccagca aaattacctc aaagagcttg acatggaata ccaggacagg
     7621 cgttcgatcc ccaaaaggtt tgccgttttc ttccaatgcg gatcaggttc agttcataaa
     7681 gagtggctga cagagaattc aaaagattgg gatctactga tcaaccatta tgatgagacc
     7741 tatctcaaca aaatatcatg cgatgtggaa tttcagcaaa caggtaaaaa agcaggcaca
     7801 aagtctacag cgtttcacat gctgctggaa agatggcctc agttacttga gccatatgat
     7861 tacgtccttt tgctcgatga tgatattttg cttgaggaaa aggatattag ccgtcttttc
     7921 catatcgcca gcgagcataa tctccatctt gcacaggcga gtctttcgtc caactcaaat
     7981 tgtgctcatc aaatttttaa gaacccggga aaaaccggca ttcgctatgt agatggggtt
     8041 gaaatcatga tgccactttt gtccaaacga gccattgatg tgggtcgaca tttatttcgt
     8101 caggccatca gcggttgggg cgtggatttt gtgttaggga aattgctttc tgcaaaaggt
     8161 ggggcggcca tcatcgatga tgtgatcgtc tggcacacca aaccaatcaa tgttgaagaa
     8221 ggtgcttttt atagaatgct tcatgaggcc tatatatatc cggaaatcga attaacgaat
     8281 cttcaaagga tatacggcgt tggacgggcg ttcaatcatg tttagcggca atttattggg
     8341 tttcctggag aacctatggc aatagcggtt gcaacagatt taattcctat acatcggcca
     8401 gactccacat tcaatcagag tccaatggca gtggctctcg cactcgtaaa aaacgaaggt
     8461 gatattatcg ctgcctggct ttcacatatc tgtgagttat ttgatttggt ttacatcgct
     8521 gaccatcagt caacggatgg tacacgggaa tatctgctcg atcaggccaa atcacaaaat
     8581 aaaatacatt tgttttcctt tgaaggaaca gggtattttc aatcagaaat aactaacaaa
     8641 ctaacacaga ttgcgatcga ggaaaattct gatgtctggt tgtttcccct ggatgctgac
     8701 gaatttttgc cgatcccaag caagctggat ttcatctctc atctgaacaa gttcaagagc
     8761 gaccagattc ttttacttaa ttggcagaat cacattccgc tctacttgca atatgatcag
     8821 gaaattaatt tcaatacccc gtttcttcgc ccatcccagc cagggctgta cgataaagta
     8881 gcggtcaaat cgggcacgat tcggaaaatg aattggacct tcacccaggg taaccatgcg
     8941 gtggcaactg aagcaggaac gctggcaggt ttagatgcca aagtccggtt cagcagtttg
     9001 atccacattc caattcgcag cgtggatcac tttacactga agtgtattca ggggaatatt
     9061 gcaacctacc agctagccaa ttggcgaacc agagggcctc ttcactggca tgagatgctt
     9121 acatccagct taagatctgg ctccatcaac cccgatctga ttcgggggtt tgcgcagtcc
     9181 tatgggagta tgaacaatcc aatgcctgtt agcgtcagtg tttacgacct tattgatgcc
     9241 ggctggaatg taaatacttt gaacatagca catgaaaatg tagtagaggg ttccttctct
     9301 cgacgcaaga aatttatcca actggcgact gaaatactgg aagccactca ggaaatttct
     9361 gatctacagc gctttttaca gttgagcaag gacgagcaaa aaagtgcgat ggatgcttct
     9421 tcatggcaaa aaggatcgat tccaaacagc ataaaattca ataaactccc tgattttgat
     9481 gcaaaagatt tgcatcctga atcttccgaa tctgagttgc tgcaggtttt tgtttccagt
     9541 agctttaccc ctcgagagat cccgatgccg tcgacctggg aaagtcatgt cccgtttttg
     9601 tattgtctct tgaatttttt cgaaccacgc cgctttgtag aattgggcac acatcacggc
     9661 aattgttttt ttgcagcctg ccagatcgcc aaacaaccga atcgctccat agaatgtatt
     9721 gccatcgata catgggccgg tgatgagcat actggaagct atggaaataa aatctttgac
     9781 gagttcaaat acatcttgag acgtgatttc ccacaaggaa aatacattcg taagtttttt
     9841 aatgatgctg ccaggcaatt tgaacagggt tccatcgatc tgcttcatat cgatggatta
     9901 cacacctacg aagctgtagc ggaagatttt gcaacctggt tgcccatgct ttcgaaccgt
     9961 ggaatcatta tgtttcatga cacgtacgaa cgtgccagag ggtttggtgt ttggaaatta
    10021 tgggaggaag tgagccgaaa atatccgtct cttgaatttg agcacggaca cggcttgggt
    10081 gtattactgg ttggaaataa tcccgatcca catataaagc gattgttcga aattgtttct
    10141 aaaaaggaaa atgcagattt tttgcgtttc tttttttcca atattggcgg tctctcgcca
    10201 atgacgatta gaaaatagat tcgggttcca ataataaatt tgaacgcaat tgatggacga
    10261 tccatggcga aagatttagt gcagcagccc actccatcat aataagtaac atgacacaca
    10321 gactatgcgg attatagctc tcacgcggta tgagcgactc ggctccagca gccgagtccg
    10381 tttttatcag tacttttctt acttaagatc acaaggcgta aagatttcga acgagccgtt
    10441 tttcagtgat cggtatatac aggatcttta tgcaggccgt tccgtttcgt taaaagattt
    10501 gctggtcgcc tatatcaagc gattggctgc attaaagcaa cgatcagcgg ttgatctgct
    10561 ctgggtcgag aaggaactcc tgcctatgtt tccagcggga cttgaggcgt tgttccgctc
    10621 atccaaaatt ccctatgttg ttgattacga tgatgccgtg ttccaccgct atgagctgca
    10681 tcccaatcga ttggtccgca ggtttcttaa acataaaatt gatgctgtga tgggccatgc
    10741 cgcgctggta attgcaggaa atcaatattt agccgaccga gctgtagcag ctggcgctcc
    10801 gaatgtcaca atcctaccca gcgttgtgga tgtaagccaa tatctaatta gacaaaaagt
    10861 aacaggcccg gtttgcagca ttggttggat tggttcaccc gtgactgttc caaacctgga
    10921 actgattcgg gatgcaatca atttgcttca tcgcgaaatg gattttcgga ttgaactaat
    10981 tggagccgga actgccaatc cgttccctac tatccccacc acaagtttgg cgtggagtga
    11041 agaaggcgag ctttccatag ggcagaagtt cgatgtgggt gtgatgccgc tgattgacgg
    11101 tccgtttgaa cgcgggaaat gcggttataa gctggtgcaa tatatggctg gagccattcc
    11161 cgtgattgct tcaccagtag gtgtgaatcg ccagatggtt gagcagggtc gcaatggcta
    11221 tctggctgat tcaaccgaag attggcttct tgctttacga gaacttttaa gcaataggca
    11281 aaaaaacctt gaaatggggt tagctggccg tcacaaagca gaacaattgt ataatcttca
    11341 agtgactgca cccaaactgc tgaatttgct atctagcgca gtaaaagtat agtgtggcgt
    11401 acgtccacaa atggactcat gctaaagatc cttctttttg ccaacacaga ctggtacctg
    11461 tacaactttc gcctttcgct tgccaaagat ctgcgtgccc agggacacga ggtcatcctg
    11521 ttatcaccgc ctggtccctt tcagaaactc ctgcgcgagc atggtttcca gcttgtttcc
    11581 ttcccgttct cgcgccaggg gattaacccg ttctatgaaa tttggacgct ctggcagttg
    11641 atccgcacct atcgcacagt tcgccccgat attgtccatc atttcaccat taagtcggtc
    11701 atttacggat cattggcggc ccactttcta cggatccgtg gcattatcaa ttccatcacc
    11761 gggctaggtc atcttttcat cgaccctggt cctgtcaccc gtttattgcg cgttgttgca
    11821 aaatggctat acaggcttgg tttgcgaaac acgcaggtta tcttcgagaa tccggaagac
    11881 cgtacaatct tcatccagaa tcatttgttg aaagcggcaa attcacatct cattctggga
    11941 acaggtgtag atgtggagaa attcagtcct gtaagcaaga agaacgatat tccggtggta
    12001 cttttctcca gcaggctact ttctacaaag ggcgtgcttg aatacatgga agctgtgcag
    12061 attctcaaac agaatggatt caaagcccgc ttcgcgctcg caggggcgac agatcccggt
    12121 aaccccgcgt cggtgacgga tgaacaaatc gaatcgtgga gtcgatctgg attagtggaa
    12181 tggtgggggt ggcgggatga tatgccaaac acactcgctc agactgatat cttctgcctt
    12241 ccatcgtacc gagaaggggt gccaaacgca ttaatcgaag cttgcgcagc tggcataccg
    12301 atcgttacca ccgacgtacc aggctgccgg gatgttgtaa cacacggcgt caacggactg
    12361 ctggtgccag tgagaaatgg aactgcatta gcaggtgcat tggagacgct attggtcaac
    12421 ccggaaatgc ggcaggaaat ggggaaagcg ggtcgtgaag cggcaatcca taaattcagc
    12481 gacaccaaag tgaatgcgga aacgttgcgt gtgtataaag taataattcc tgccaatcaa
    12541 ccggggaaaa ttggcgcaga ttagcctgca tgctaaaata tgaatatcta cagtatgtag
    12601 gatagccttg atgcaaaaaa ttaaaggatc gcaacgaatt ttattagcct attctttatt
    12661 tgtcgcgatc atttcgttgc tttttctacg gggaatgatt ctctcgccat ccgaatctgg
    12721 tagcgcagta tttcttggtc tctcaattcc acggcttgtt atttcggtgg gagtcttaat
    12781 cacatggatc ttttatgttg caatagcagt gaaggcagca cggaatcacg aatgggctga
    12841 aaaagcactg gagtattggt ttggaggagg gcccgctggc acggtcgcac gctggatagc
    12901 cggtatcagc tttgggctgg gatggattgg ctgttttctg cctgcgtatc gggttggagc
    12961 cttcggcaat tattggatca gcctgcgccc cattatgatt tttatcatga tagttagcgt
    13021 agcgacatta acagtcagtt ttatcaaacg
//
LOCUS       DASTJP010000588         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_996627, whole genome shotgun sequence.
ACCESSION   DASTJP010000588 DASTJP010000000
VERSION     DASTJP010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996627"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="VFQ23_17370"
     CDS             complement(<1..99)
                     /locus_tag="VFQ23_17370"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9908423.1"
                     /translation="MPENILIAIAWPYANAEIHVGNITGSHLPGDIV"
     gene            complement(314..817)
                     /locus_tag="VFQ23_17375"
     CDS             complement(314..817)
                     /locus_tag="VFQ23_17375"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET9908424.1"
                     /translation="MANPRKPFRINVGFIIHEEIGYSHEIPFELDKVKLDDLELRDFE
                     GVVYIGRTPQGLIVQGKFSAKTTLECVRCLKKFNYPLKWEITELYAFNKKSVSESGLL
                     LPDDAHIDLALLLREYALLEVPINPLHDPECKGLCIECGQDLNVRDCGHSQLEDDSPF
                     SKLKDLL"
     gene            complement(878..1846)
                     /locus_tag="VFQ23_17380"
     CDS             complement(878..1846)
                     /locus_tag="VFQ23_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9908425.1"
                     /translation="MNTVSFFTSVQDQLQFIERRIREQADDQNHSDLRSALSHLLAAG
                     GKRIRPTLGLLVGNMLGAPEEKLITLGAAVELLHTATLVHDDLIDGSLLRRGMPTLNA
                     RWSPAATVLTGDFLFARAAKLAAETDYLPLMKLFADTLATIVSGELTQLFSARGMINR
                     QNYYQRIYAKTASLFEMSALAACMVATEDEDIRDSMKSFGYEVGMAFQIMDDVLDFTG
                     DPSAVGKPIGSDLLNGLVTLPAIYYAETNPHEADIISLPEGGWKDTERVERLVEAIRN
                     SPAIEQAREEARQAICRALNALEEAPVSADREALENLANFIVDRKI"
     gene            complement(1850..>2285)
                     /locus_tag="VFQ23_17385"
     CDS             complement(1850..>2285)
                     /locus_tag="VFQ23_17385"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9908426.1"
                     /translation="REENDEWFGPASIDLLEILKEWNQELVRQNHTLVDRPGVLHSYN
                     GNLETALKALEMNFYIGITGPVTYKNATEKRNIISQLPLTSLLIETDAPFLTPVPHRG
                     QRNEPAFVRVIADKIAEVHMTTLEMVAEITMKNANHLFGWGG"
BASE COUNT          578 a          554 c          548 g          605 t
ORIGIN      
        1 gacaatgtcg cccggaaggt gtgagccggt aatatttccg acatgtattt cagcattggc
       61 atagggccag gcgattgcaa ttaagatgtt ttcaggcatt catgcctccg aattttatat
      121 tgtaggggca ggcagctgcc ttcccctacg agaggtctaa ataaacaaaa aggctgtcct
      181 aaacttggac agcccgtcga ctttcagtta cgacgatcta acgtgctctc caggcgcgag
      241 gtaatttatc catgcccata ggcattgacc tcaaaattgt cgctgaagag tagtgtgtag
      301 ccatgaacat attttacaac aaatctttga gtttggaaaa gggagaatca tcctctaact
      361 ggctatgccc gcaatcacga acattgagat cctgaccaca ttctatgcag agtcctttac
      421 actcaggatc atgcaatgga ttgatgggaa cttccaacaa tgcatattca cgcaacagta
      481 gggccaggtc aatgtgagca tcgtctggaa ggagtagccc ggattcactt actgatttct
      541 tattgaaagc gtataactca gtgatttccc atttcaaagg atagttaaat ttcttcaggc
      601 accgaacgca ctcgagcgtc gtcttggctg agaattttcc ctggacgatc agtccctgcg
      661 gcgtgcgacc gatataaact accccttcaa aatctcgcag ctcaagatcg tcgagcttaa
      721 ctttatctaa ttcaaatggg atctcgtgac tatagccgat ttcctcatgg atgatgaaac
      781 cgacgttaat gcgaaagggc ttacggggat ttgccataat acacctaaag actttgaaag
      841 gatcgaccgg tgaagtgacg attcttctga attcgaatta tatttttctg tcaacaataa
      901 aattcgcgag attctccaag gcttcgcgat cggcgctgac tggcgcctct tccagggcat
      961 taagcgcgcg gcatattgcc tgtcgcgctt cttccctggc ctgctcaatc gccgggctgt
     1021 tcctgatggc ttctacaagc ctttcaacac gttccgtatc tttccatcca ccttccggga
     1081 gagatatgat atcggcttcg tgtggattcg tctctgcgta ataaatcgcg ggcaaggtga
     1141 ccaatccatt gagaagatca gagcctatcg gcttcccgac tgcagatgga tccccagtga
     1201 aatcaagaac atcgtccatg atctgaaatg ccattccaac ttcatagcca aaggatttca
     1261 tcgaatcacg aatgtcttca tcttcggtcg ccaccataca ggctgcgagg gcagacattt
     1321 caaataacga agccgttttg gcgtagatgc gctggtaata attttgccgg ttgatcatcc
     1381 cgcgcgcaga aaataattgg gtcaattcgc cactgacaat ggtcgccagt gtgtctgcaa
     1441 aaagcttcat cagaggtaga taatctgtct cggcggcgag tttggcagcg cgcgcaaaaa
     1501 gaaaatctcc cgtcaaaacg gttgccgcgg gcgaccaacg cgcgtttaaa gtgggcattc
     1561 cacgtcgaag aagggagcca tctatcagat catcgtgaac aagagtggct gtgtgaagta
     1621 attcgacggc cgcaccaagg gtgatgagct tctcctcggg tgcgcccagc atgtttccga
     1681 caagtagacc aagagttggt ctgatgcgct tccctccggc tgccagcaag tggctcaacg
     1741 ccgaacggag gtcagaatga ttttggtcgt ctgcctgctc tcgaatccgt ctctcgatga
     1801 attgaagctg gtcctgaacg gacgtgaaaa aactaactgt gttcaaatgt cagcctcccc
     1861 aaccaaataa gtgatttgca tttttcatcg taatttctgc gaccatttcg agggttgtca
     1921 tgtgaacttc tgcaatttta tcagcaatta ctcgcacgaa tgccggttca ttgcgctgac
     1981 cccgatgcgg taccggtgta agaaatggcg catcggtttc aatgagtaag ctggtaagcg
     2041 gcagctggct gatgatgttt cttttttctg tggcattctt gtacgtgacc gggccagtta
     2101 tcccaatata gaagttcatt tcgagcgcct taagagcagt ttcgagattt ccattatagg
     2161 aatgtagaac accgggcctg tcgacgaggg tatgattttg ccgaaccaat tcctggttcc
     2221 actccttcag aatttcgagt aagtcgatgg aggctggacc gaaccattca tcgttttctt
     2281 cgcgc
//
LOCUS       DASTJP010000589         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_362826, whole genome shotgun sequence.
ACCESSION   DASTJP010000589 DASTJP010000000
VERSION     DASTJP010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362826"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFQ23_17390"
     CDS             <1..485
                     /locus_tag="VFQ23_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET9908427.1"
                     /translation="LGYVFEGKARRPFLPAKADELGVPFGPERRDLVAGKEITLPDGR
                     RVNPDDVLGPVQKGVKLVVVGDAGKTDDLLEVSRDADALVIESTYLDEEAEMARQFSH
                     LTARQGAELAVKAGVRKLILTHISRRYREKDVLKEAQAIFPNTAVARDFDSFQIKRDE
                     "
     gene            complement(564..1766)
                     /locus_tag="VFQ23_17395"
     CDS             complement(564..1766)
                     /locus_tag="VFQ23_17395"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET9908428.1"
                     /translation="MSTHTKGVIGILTGGGDVPGLNPAIRAVTIRATREGYKVIGIRR
                     GWAGLVDIVRDHNADNSNNFQVLTEEIVNKAGRTGGTFLHTSRTNPSRVRTDRLPPAL
                     QEKYKDEVNDLTPEVLKNIDFLGLDYLIPIGGDDTLSYGVRMYQEGMKVVAIPKTMDN
                     DVPGTDYCIGFSTCVTRTIEMANSLRTVAGSHERLLVLEVFGRYAGFTAMLPTMAGAS
                     HRCVIPEYKFNIEQLVELMMYDRKHNPSHYSVVLVSEGAMFEGGEMVFQDRSTDDFGH
                     MKLGGIGDLISAELKQMTASHNNGRMVDTVNQKLAYLVRCGDPDAIDSIVPMAYGNLA
                     LDLILKKVHGRLVVLKNGRYDNIPIDIVTSRKKTVNVERFYNTERLRPKFESFEMNPL
                     FIMTSESI"
     gene            complement(1861..>2441)
                     /locus_tag="VFQ23_17400"
     CDS             complement(1861..>2441)
                     /locus_tag="VFQ23_17400"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908429.1"
                     /translation="AKGNYTTRLIDMGRVNDRLFLLRIGIGLEADMMKSASQEVKNRF
                     GMLAYAFSALDEMRSLTPTSYRVRVDGELHEVEGISCMIANSGNMAIGGLTLSKKIDV
                     SDGLLDVVVFRSADLAAMVTITAAVMSENAEATDNPQLEHYQGREIVVEANPPQTISI
                     DGEVIEPVTLTASILPQAVHVIVPTIEAVEKN"
BASE COUNT          582 a          638 c          596 g          625 t
ORIGIN      
        1 gcctggggta tgtttttgaa ggaaaagcgc gacgtccatt tctacctgcc aaagcggatg
       61 aacttggggt tcccttcgga ccagaacgcc gcgacctggt agccggtaag gaaatcacgc
      121 tgcccgatgg aaggcgcgtc aatccggacg atgtgctggg tcccgttcaa aagggtgtga
      181 aactcgtggt agtgggtgac gccggaaaaa cagacgatct gcttgaggtc agcagagatg
      241 cggacgcgct ggtgatcgaa tccacgtatc tcgatgaaga agcagagatg gcgcgtcagt
      301 tctctcatct gaccgctcgc cagggtgcag aactggcagt aaaagcggga gtcaggaagt
      361 taattcttac acatatctca cgccggtatc gcgagaagga tgtgttaaag gaagctcaag
      421 ccattttccc aaacacagcc gtcgcgcggg atttcgattc attccagatc aaacgcgacg
      481 aatagaactt cttaaatctt aaagtaaaag acacgaagga aaagaatggc ccttcgtgtc
      541 tttgtgtcaa attttcgaaa taattagatt gattcgctgg tcataatgaa cagcgggttc
      601 atctcaaaac tttcaaactt tggtcgtaga cgttctgtgt tatagaaccg ttctacattg
      661 accgtcttct ttctacttgt tacgatatcg atggggatat tatcgtagcg accatttttc
      721 agaacaacca gtcttccgtg taccttcttg aggatcaggt caagtgccag gtttccatac
      781 gccattggga caatagaatc gatcgcatcg gggtcaccgc aacggacgag ataagccagc
      841 ttttggttaa ctgtatccac catgcgccca ttgttgtggc tggccgtcat ttgtttcagc
      901 tccgccgaga tcaggtcgcc gatacctccc agcttcatgt gcccaaaatc atctgtcgat
      961 ctgtcctgga agaccatttc accgccttca aacatcgcgc cttcagacac caacaccact
     1021 gagtagtggc tgggattgtg cttgcggtca tacatcatca actcaacgag ctgctcgatg
     1081 ttgaatttgt attcggggat cacgcagcga tgggaggcgc ccgccatcgt ggggagcatg
     1141 gcagtgaacc cggcataacg cccaaacact tccagtacca ggagtctttc atgagagcct
     1201 gctacagtcc gcaggctgtt cgccatttcg atggtacgcg tcacacacgt gctgaagcca
     1261 atgcagtagt ctgtgccggg cacatcgtta tccatcgtct tcggaatggc aacgactttc
     1321 atcccctcct gatacatccg tacgccataa ctcagggtgt catctcctcc gattgggatg
     1381 aggtaatcga gacccagaaa atcgatgttc ttcaatactt ccggtgtcaa atcgttgact
     1441 tcatccttgt acttttcctg tagcgcgggg ggaagccgat ctgttcgaac cctggacgga
     1501 ttcgtgcgcg aggtatgcag aaaggtacca ccggttcgcc cggctttatt gacgatctct
     1561 tctgtcagca cctggaagtt attgctgtta tccgcgttgt ggtcgcgaac gatatccact
     1621 aaccctgccc agccgcggcg aatgccgatt actttatagc cttcgcgtgt ggcacgaatg
     1681 gtcaccgcgc ggatcgccgg gttgagcccc gggacatcgc ctcctcctgt tagaatgccg
     1741 atcacgcctt tcgtatgtgt gctcataatg acctctcaat gatattggtt gtaatgattt
     1801 tttcagacca ggaattgcag ccttgaaaac gatccctgga ttgaatgcta tataagaaac
     1861 tcagtttttc tcaactgctt cgattgtggg aacaatgaca tgtacggctt gtggcaaaat
     1921 actggccgtc aacgtgacag gttcaattac ctcgccatca atggagatag tctgtggcgg
     1981 gttcgcctcc acgacaattt cacgcccctg ataatgttcc aactgcgggt tatctgtcgc
     2041 ttctgcattc tctgacatta ctgctgcagt aatagtaacc atggcagcga gatctgcact
     2101 ccgaaatacc accacatcca ataagccatc gctgacatcg attttttttg acagtgtaag
     2161 acctccaatt gccatattgc cggaattggc gatcatacag cttatgcctt ccacttcatg
     2221 tagttcacca tccacgcgca cgcgatagct tgtgggcgtc aggctgcgca tttcgtccaa
     2281 cgctgaaaaa gcataggcca gcatcccaaa gcggttttta acttcctgac tggcgctttt
     2341 catcatgtcg gcttccaacc caatgccaat gcgcagcagg aacaagcgat cattcacccg
     2401 gcccatatcg atcaggcgcg tggtgtaatt cccttttgca a
//
LOCUS       DASTJP010000590         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_584842, whole genome shotgun sequence.
ACCESSION   DASTJP010000590 DASTJP010000000
VERSION     DASTJP010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584842"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VFQ23_17405"
     CDS             <1..1265
                     /locus_tag="VFQ23_17405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HET9908430.1"
                     /translation="RHILPIQLSAQEREDLIAFLYALTDESALPEVPGAVPSGLPVVE
                     QVANPARELVRQFNVEGTESGASLTHEPTVINVGPNETIQQAVDRSGPGDTIQVPYGI
                     YYEAVVIDWSDIKLMGIPNENGDWPVIDGEGTRSDGVIASGNNFEMAFFDIKNYTSNG
                     VLVEGATGVYLHDLYIENTGVYGAYPVRCTDVLMERIEATLMNDAAIYAGKSENVVIR
                     DTVTYGNVIGIELENTVNGEVYNNYAYDNTVGIFVDLLPQLPSKVSLNTKVYDNVVEN
                     NNGDNFAKPDTAAALIPPGTGMLILAADEVEIYNNTIKGNKTGGLAVFNLTIGFAENE
                     IDVGPNPEHVYAHDNIYENNGYEPDPFVKNMLGRGFDIIWDTNGADNHFDETVESSFP
                     PILPKTSWPEPLYNLYWRTMNFVVGLVS"
     gene            complement(1345..2556)
                     /locus_tag="VFQ23_17410"
     CDS             complement(1345..2556)
                     /locus_tag="VFQ23_17410"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9908431.1"
                     /translation="MDLDNTDDSVTRRIRAIVDRVSMAKENDLYFYNQPVEELRGGAK
                     VLVRGREMGMYASYGYLGLLNHPRINEAAKKAIDKFGTGTNGVRSLAGTLTIHTELEE
                     TIANFKHTDGAVTFSSGYATNLSVVSTLMGRGDYVFSDKLNHASIVDGCLMSGAEFRR
                     FRHNDMEHLEGLLKNAPADVTKLVIADSVFSMDGDIIDLPKVLELTKKYHAWLMIDEA
                     HSIGVLGEKGTGIEEHFGLGDVIDIKMGTLSKTIPSVGGYVAAKKDIITYLRHASRAY
                     IFSAALPPAQAAAANEAFKVILDEPWRIERLNQNTKQFIGGLKGMGFDTMLTETAIVP
                     VLCGDDETAFAMTREAQHNDVFVLPVVSPAVPEGLARLRATVTAAHDPSEIERAMDVI
                     GEAGKKLGIIQ"
     gene            complement(2607..>3638)
                     /locus_tag="VFQ23_17415"
     CDS             complement(2607..>3638)
                     /locus_tag="VFQ23_17415"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9908432.1"
                     /translation="QGLFSKAVITERQRHMDKVLNWGLLSTARINRALIPPLRASDRN
                     RLLAVGSRAQHSADRYAKEWNIPRAHGSYEALLADPEIDVIYNPLPNHLHAEWTIKAV
                     EAGKHVLCEKPLALTVDEVDAIKAAAQAHGRVVAEAFMYRHHPQTHKVQELIQGGSIG
                     TLKLMRGSFTYVLSNQADVRLDPAMGGGSIWDVGCYPISYALAVVGENPLEVFGWQAT
                     GATGIDDTFVGQMRFANDIYAQFDSSFSTPFRTFMEIVGTEGTLTIPKPFKPVYDEKA
                     HLTRGDHTETIEMPGQELYIGEVEDMADAILLGRESRVSLDQSRAHVAVITALLESAR
                     SGKPVTPDL"
BASE COUNT          903 a          931 c          899 g          905 t
ORIGIN      
        1 acaggcatat cctcccgatt caattaagcg cccaggagag agaagatttg atcgcttttc
       61 tgtatgcgct gaccgatgaa agtgccttac ccgaagtgcc aggcgcggtt ccatccggct
      121 tgccagtcgt tgaacaggtc gctaacccgg cgcgcgaact cgtccggcag tttaatgtgg
      181 agggaacaga atcaggcgca agtctcacgc acgagccgac ggtgatcaat gtcggtccga
      241 atgaaactat ccaacaagct gtggatcgtt ctggtcccgg cgatacaatc caggttcctt
      301 atggaattta ttatgaggcc gtggtcatcg actggagtga tatcaagcta atgggcatcc
      361 ccaatgaaaa tggggattgg cccgtgatcg atggtgaagg gacacgttcg gatggcgtga
      421 tagcttcggg aaataatttc gagatggcgt ttttcgatat aaagaattac acgtccaatg
      481 gcgttcttgt ggaaggtgct acaggagttt acctgcacga cctatatatt gaaaatacag
      541 gtgtctatgg cgcataccct gtgcgatgca cggatgttct gatggagcgc atcgaagcca
      601 cactgatgaa cgatgcggcc atttatgcag gcaagtctga aaatgttgtc atccgcgaca
      661 ctgtgacgta tggcaacgtg atcggcattg agctggaaaa tactgtcaat ggcgaagtct
      721 ataacaatta tgcatacgat aatacggtag gcattttcgt agatctattg ccacagcttc
      781 catccaaggt ttcgttaaat acgaaggttt atgacaacgt tgttgagaac aacaacggtg
      841 ataatttcgc gaagcccgat acggctgcgg cgcttatccc tcccgggaca ggtatgctga
      901 tccttgccgc ggatgaagtt gaaatctata acaatacaat caaagggaac aagaccggcg
      961 gtctggctgt cttcaatcta accatcggat tcgccgaaaa tgaaatagac gtaggaccca
     1021 accctgaaca tgtatatgcg cacgataaca tctatgaaaa caatggctat gagcccgatc
     1081 cgtttgtgaa gaatatgctg ggtcgtggct tcgatattat ttgggatacc aacggcgcgg
     1141 acaatcattt cgatgaaact gtagagtcat cattcccgcc cattctgcca aagacaagtt
     1201 ggcccgaacc actgtataac ctgtactggc gaacgatgaa ttttgttgta gggttggtaa
     1261 gctaggcata aaaaggacca gatttttgtc aaatctggtc ctttttattt cgactgctcc
     1321 cttctatgga agcatgtagc cttcttattg gataatccct aatttctttc ctgcttcacc
     1381 gatcacatcc atggcacgtt cgatttcgct ggggtcatgt gcggccgtaa ccgtggcgcg
     1441 caggcgagca agtccttctg gcaccgcagg tgagacgacc ggcaacacaa acacatcgtt
     1501 gtgttgcgct tcgcgcgtca tagcaaatgc tgtctcgtcg tcaccacaca gtactgggac
     1561 gatggcagtc tcggtcagca ttgtatcgaa gcccattccc ttgagcccgc caatgaattg
     1621 tttggtattc tggttgagtc gttcaatgcg ccagggttca tccagaatca ctttgaaggc
     1681 ttcgttcgcc gcggcggcct gagccggggg aagcgcggct gagaaaatgt aggcgcggct
     1741 ggcatggcgc aaataggtga tgatatcctt cttcgccgca acgtaaccac ccaccgaagg
     1801 aatggttttg cttagcgtgc ccatcttgat atcaatgaca tcgccaagac caaagtgttc
     1861 ttcgattccg gtgccctttt caccgagcac gccgatcgag tgggcttcat cgatcatcag
     1921 ccatgcatga tattttttcg tcagttccag cactttcggc agatcgataa tatctccgtc
     1981 catagaaaaa accgagtctg caatcacgag cttggtcaca tcggccggcg catttttcag
     2041 cagcccttcg agatgttcca tgtcgttgtg gcgaaaccgt cgaaactccg caccggacat
     2101 caaacatcca tctacaatgg aggcatggtt tagtttgtcg gaaaaaacat aatcgccgcg
     2161 ccccatgagc gtggaaacga ccgataggtt tgtagcatac ccagatgaaa acgtcacagc
     2221 accatctgta tgtttgaaat tggcaatcgt ttcttccaat tcagtgtgga tggtcaacgt
     2281 tccggccagc gagcggactc cgttcgtgcc cgtgccgaac ttatcaatcg ctttcttcgc
     2341 ggcttcattg atacgcggat ggttaagcaa tccaaggtag ccataagacg cgtacatccc
     2401 catttcacgg ccacgcacca gaactttcgc cccaccccga agctcctcca cgggctgatt
     2461 gtaaaaatag aggtcatttt cctttgccat tgaaacacga tccacgatgg cgcgaatgcg
     2521 acgggttact gaatcatcag tgttatctaa atccatatag ggacctccaa aaaagattgg
     2581 cgtaagttta ccagagactt atgaagttat aaatccggcg ttaccggctt cccacttcgc
     2641 gctgactcaa gcaatgccgt gatcacagca acatgggcgc gactctgatc taaggagact
     2701 cgtgattcac gtcccagcag gatcgcatcg gccatatctt cgacttcacc gatgtacagc
     2761 tcctgccctg gcatttcgat ggtctctgta tgatcgccgc gcgtcaaatg agccttctca
     2821 tcatagacgg gcttaaaggg tttgggaatc gttaatgtgc cttcagttcc tacgatttcc
     2881 ataaaggttc gaaacggagt agaaaagcta ctatcaaact gggcatagat gtcatttgca
     2941 aatcgcattt gcccgacaaa cgtatcatca attcccgtcg cacctgtcgc ctgccagccg
     3001 aaaacttcaa gcggattctc ccccacgacg gccagtgcgt agctgatcgg gtaacatccc
     3061 acatcccaaa tacttccgcc gcccattgcg gggtcaagcc tgacgtctgc ttggttgctg
     3121 agaacatagg taaacgaacc gcgcatcagt ttcaacgtcc caatcgaacc accctggata
     3181 agttcctgca ctttgtgagt ttgaggatga tgccggtaca taaaggcctc ggcaaccacc
     3241 cgtccatgcg cttgcgccgc cgcttttatt gcatcgactt catctacggt cagggcaagt
     3301 ggcttttcac ataagacatg cttgcccgcc tctacggctt tgatggtcca ctcggcatgc
     3361 agatgatttg ggaggggatt atagatcacg tcgatctcag gatccgcaag caatgcttca
     3421 taggaaccat gtgcgcgcgg gatgttccat tcctttgcat atcgatccgc tgagtgctga
     3481 gcgcgcgagc caaccgccag aagtcggttt cggtctgaag cgcgcaacgg aggtatcaag
     3541 gcgcgattaa tacgggccgt cgaaagcaat ccccagttta gaactttatc catgtgtctt
     3601 tgcctttcgg tgataacagc tttagaaaat aatccctg
//
LOCUS       DASTJP010000591         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1228165, whole genome shotgun sequence.
ACCESSION   DASTJP010000591 DASTJP010000000
VERSION     DASTJP010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228165"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..544)
                     /locus_tag="VFQ23_17420"
     CDS             complement(128..544)
                     /locus_tag="VFQ23_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET9908433.1"
                     /translation="MTKKIEAIFREEKLNDVKEALYNIGIIGMNVIEVRGHGRQGGIA
                     LAGRTGTYRVDMLPRVQLNIILSDHNVEKTVDAIQQAAQTGNIGDGLIFVYPVEEVIR
                     IRTGEQGLDALSYEGDIDARKQVVEIDLVNAVGISD"
     gene            complement(783..2006)
                     /locus_tag="VFQ23_17425"
     CDS             complement(783..2006)
                     /locus_tag="VFQ23_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET9908434.1"
                     /translation="MDAINAGDTAWILIATALVMVMTPALAFFYGGMVRKKNLLSTIN
                     LSFITIGLISLQWVLIGYTLAFGKSVGGFIGGLDFLGFANVGAEPNADYAGTIPHMAF
                     AAYQMMFAIITPALITGAFVERIRFKAFLIFTLLWATFVYDPVAHWVWGVGGILRSIG
                     ALDFAGGTVVHITAGFSALAFAIVVGKRKWFGQAAMEPSNIPFTVLGAGLLWMGWFGF
                     NGGSALAANGLAVNAVVTTNTAAAAAAVVWMFLSWRDNKPSILGIVTGAVVGLVAITP
                     AAGFVTPLAAIAIGGLAAPISFYAIRLRQKWGLDESLDVWACHGMGGTWGALATGLFA
                     TKLVNSAGADGLFYGNPSLLLTQAIAVGVSIIFSFAVTFILTRLLRGSMGLRVTENEE
                     EVGLDISEHGERAFA"
     gene            2332..3561
                     /locus_tag="VFQ23_17430"
     CDS             2332..3561
                     /locus_tag="VFQ23_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9908435.1"
                     /translation="MKASLLALCLISIFISSCGTSPTILPSSTPAPTIVKVATASAIF
                     TAIPTETAFPTVSPTPHAIIEPGFIVSLEKQQRTIGSSLTFSPDGLILAQADASVKLW
                     DVSTHRLIRELKYPDYEKYYATQALFSPDGSLIVVSITDYLTNLEKPNSHLLVWDIST
                     GELQQDWTQEYAVMSAYDGFQPEPRVYNIPVDAMAFFPASTKLAYANGNRIEIRDVLA
                     GGESTGWSLGDTMYASEISIRGDGEFLYILMRWYKDLNFPALYRYKFTAQIWHPATKS
                     LRRAIKFEEVYPGKEDVWLVGEHLLHQDTIKPALEVLDLSTDEKREFPYRIGSKFFNE
                     DASLMLVYRSIRVDEDEEGLELWETDTWRNIHTFTPNFGDEWYFLSNIVFSPDNSSLA
                     IEYDGQVSLWDIRSVQP"
BASE COUNT          975 a          953 c          887 g          911 t
ORIGIN      
        1 caatatgtca cgttggttga gtaggcggtg cttgtccgcc gtatcgaaac caacaggaaa
       61 ctcaaactgc ctggatttgt ggtttcgata cgccctgcgc taccgcttcg ggctactcaa
      121 ccaccactca gtccgaaatt cccacggcat tcaccagatc aatttccacg acctgcttgc
      181 gggcatcgat gtctccttca tacgataggg catcgagtcc ttgctcgccg gtgcggatgc
      241 gaatcacttc ctcgaccggg tagacgaaga tcaacccgtc gccgatgttc ccggtttgag
      301 ccgcttgctg gatggcatcc acagtctttt caacgttatg atcgctgagg atgatgttga
      361 gttgtacgcg tggcaacata tccacgcggt aggtgcctgt ccgtcctgcc agcgcgatgc
      421 cgccctgacg tccatgcccg cgcacttcaa tcacgttcat accgatgatc ccgatgttat
      481 agagcgcttc ctttacatcg ttgagttttt cctcgcggaa gatggcttcg atttttttcg
      541 tcatgattcg attcctttct ttaactttag taacttacct ttcgcgtcga gcggaggctt
      601 tcgtgcttgt tggaagccgc agacgagacg cagaagtttc atgaaaattc cgcttcgtct
      661 ttgggtttcg atacgtcccg caaacagcat gcgggactac tcaaccctcc gctaccctac
      721 gggcacgaca cttgcaccgc gctgcggatg cagtgcaggt gtgtcagcgc gaaatatctt
      781 agttatgcaa acgcccgttc gccgtgttcg ctgatatcga gaccgacctc ttcctcgttc
      841 tcggtgacgc gcagtcccat ggagccgcgt aagagccggg tcaggataaa ggtcacagcg
      901 aaagagaaaa tgatggatac acccacggca attgcctgtg tgagaagcag gctcggattc
      961 ccatagaaca agccatctgc gccagccgag ttcacgagtt tcgtggcgaa caaaccggtc
     1021 gctagagcac cccaggtgcc gcccataccg tgacaagccc acacatccag cgactcatcc
     1081 aatccccatt tctggcgcaa tcgaatggcg tagaagctga tcggcgcagc caacccacca
     1141 attgcgatgg cagccagcgg cgtgacaaag cctgcggccg gtgtgatcgc gactagcccg
     1201 accaccgcgc cggtcacaat gccgagaatg ctgggtttgt tatcgcgcca gctcaggaac
     1261 atccacacca ccgccgccgc ggctgctgcg gtattcgtcg tcaccaccgc attgacggcg
     1321 agtccgttag ctgccagggc actgccaccg ttgaaaccaa accatcccat ccacaataga
     1381 cctgcaccca aaacagtgaa cggaatattg ctgggctcca tggcggcctg cccaaaccat
     1441 ttgcgcttgc caaccacgat cgcgaacgcc agcgcggaga atcccgcggt gatatgaaca
     1501 accgttccac cagcgaagtc cagcgcgcca atgctgcgta agattccgcc tacgccccaa
     1561 acccagtgcg cgaccgggtc atagacgaat gttgcccaca aaagcgtgaa gatcaggaac
     1621 gccttgaagc gaatccgttc gacgaaggct ccggtgatca gagcgggagt gataatggca
     1681 aacatcatct gatacgcggc aaacgccata tgcgggatgg tgcccgcata atccgcattg
     1741 ggctctgctc ccacgtttgc aaaaccgagg aaatccagcc cgccaatgaa acctcccaca
     1801 ctcttcccga acgccagcgt atagccaatg agcacccact gcaagctgat caaaccgatc
     1861 gtgatgaaac tcaaattgat ggttgagagc aaattctttt tgcgaaccat gccgccgtag
     1921 aagaaagcca gcgccggcgt catgaccata accaacgccg ttgcgatcag aatccaggcg
     1981 gtgtctccag cattaattgc atccattgtg tttctctcct tcactccaat aagttgcagg
     2041 acgggtgaga gtcacccaaa tccattcagg ctattttatg gaaatgaagg tgtagtgcta
     2101 ttccccgaat gggtaattat tctactgttg cgtgtaacaa attggatgag tagcggtgag
     2161 tcaacacgta tgcgcggatg tataatttgc gcatcgccgg gcttcggagt ttttatgtat
     2221 gtccgtctaa aatcctaaat gatttaggga atgaggcggc tcagagttcg tctaatctct
     2281 agttgggcgc tgaaattgat aaacttgttc aagagctata aaaggaacat tgtgaaagca
     2341 tcgttgctcg cgctttgtct aatttctatc tttatatcat cttgcggaac aagtcctacg
     2401 atattaccgt caagtacgcc tgcgccgaca atagttaagg tagcaacagc cagtgccata
     2461 tttacagcta ttccaacaga aactgctttt cccactgtat cacctacacc acacgcaatc
     2521 attgaacccg gtttcattgt ttcacttgaa aaacagcaaa gaacaattgg cagtagtctt
     2581 actttcagtc cagatgggtt aattcttgcc caagcagatg cgagtgtaaa gttatgggat
     2641 gtaagcaccc ataggttaat ccgcgaattg aagtatccag actacgaaaa atactatgca
     2701 acacaggctc tgtttagtcc tgacggcagt ttgattgttg tcagtattac tgattatctg
     2761 actaatctag aaaaaccaaa tagtcatttg cttgtatggg atatttcaac aggcgaatta
     2821 cagcaagatt ggacgcaaga atatgctgtc atgtccgcgt acgatggatt tcaacctgag
     2881 ccaagagttt ataacattcc tgtcgatgcc atggcttttt tcccagccag cacgaaacta
     2941 gcttatgcta atggcaatcg tattgaaatc agagatgttc ttgcaggagg agaatctacc
     3001 ggctggtcgc tgggagatac gatgtatgct tctgaaatat cgatacgcgg cgatggtgag
     3061 tttttgtaca tcttaatgag atggtacaaa gatttgaatt tccctgctct ttatcgctat
     3121 aaattcacag ctcagatctg gcatccagct acaaagagtc ttcgacgagc gatcaagttt
     3181 gaagaagtgt atccagggaa agaagatgtg tggctcgttg gagaacattt gttacaccaa
     3241 gatacaatca agcctgcctt agaagtcctt gatctttcga cagatgaaaa gcgagaattt
     3301 ccgtatcgaa ttggatcgaa attcttcaac gaagatgcta gcttgatgct tgtttatcgc
     3361 tctatccgtg ttgacgagga tgaggaaggg ctagaacttt gggaaactga cacttggaga
     3421 aatatacata catttactcc taatttcggg gatgaatggt atttcctaag taatatcgtt
     3481 tttagtcctg ataattcatc actggcaatc gagtatgatg gacaagtatc tttatgggat
     3541 attcgttctg tccaaccata atagtcttaa ggtcaaaaac tgcccaacac agcgtactaa
     3601 gaaacagaat tacggtagaa aacaatgaaa gatgtgcaag ctgcataacc ccaaaaaggc
     3661 gtcgcgacaa tttccaaaaa acgacgccga tgagatgcta gaattgttcg acatgaacgg
     3721 cacggc
//
LOCUS       DASTJP010000592         4552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_363342, whole genome shotgun sequence.
ACCESSION   DASTJP010000592 DASTJP010000000
VERSION     DASTJP010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363342"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..795
                     /locus_tag="VFQ23_17435"
     CDS             118..795
                     /locus_tag="VFQ23_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908436.1"
                     /translation="MLRRILSFSMISLAIMGAACSRLTQSPSSVIATTPKVVEKHLSA
                     FRPIEGTSYMIADISGNPQVEERGSFSPFRWIDSGYSGYSGYEIYNFVFFNSQTETYN
                     RLMLTNEFVVLQIAGFPPGTPTENPADFKPVMWWMYVLAKADTDQNGVLNYQDKLTIG
                     VTDVGGNSPTELIPNVDSVLGQILKEDDTLFVIYHSLDKNYIAKIDLPGRLVISTAEM
                     SIGEDVK"
     gene            803..1087
                     /locus_tag="VFQ23_17440"
     CDS             803..1087
                     /locus_tag="VFQ23_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908437.1"
                     /translation="MGFLKKLFGGTPAKSNKRYYVFQVRCKRCGELIEGRVDLDNDLS
                     VEFEEDREVFHVRKGLVGNNRCFQQIEVELKFDSERQLIEHQVQGGTFIE"
     gene            1164..1550
                     /locus_tag="VFQ23_17445"
     CDS             1164..1550
                     /locus_tag="VFQ23_17445"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET9908438.1"
                     /translation="MRYTSPPNQQAYYEQVWNLVRQIPSGKVASYGQIALMIPPPVDV
                     EFESYKAFSPRWVGGAMAACPDDVPWQRVINSQGKISERAGAEKQRQLLEQEGVEFDA
                     KDRVDFKKYGWKGPNEEDQPQQPSLF"
     gene            1660..2508
                     /locus_tag="VFQ23_17450"
     CDS             1660..2508
                     /locus_tag="VFQ23_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908439.1"
                     /translation="MDIYLATEHAYHFIPQFNVEVARDRVEQKKVNLIAGTLGSLFSR
                     AKPEDLQLATVENRVEPFWLLEASSRTVYDRARTYTIPSGGAEVRSVTVMGTEFPVVT
                     QPKGDNAFTVTGVEHCVQELKSRQTFDGITGAKTDLLKYAGSAKTEIADLTNFAPEGV
                     LVVPPQVKANAVVRQVTADVVQPVQNVQTIHEERVDVETIDLNFRPIYAFEYEWAAKN
                     KRVVIEFDPLTGELHTGGKRWSDQIKSMVTRDLIFDITADAFGTIVPGGSIAVKLVKA
                     VVDRKK"
     gene            2594..3088
                     /gene="dps"
                     /locus_tag="VFQ23_17455"
                     /gene_synonym="pexB"
     CDS             2594..3088
                     /gene="dps"
                     /locus_tag="VFQ23_17455"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HET9908440.1"
                     /translation="MKTKTKMLNTNNDLPANTREQIIKLLNQELADMSDLHSQTKQAH
                     WNVKGEHFISLHKLFDEFAEALTGYIDEIAERAVQLGGTAMGTNRMSAAESRLPEYPS
                     EVMGSMDHVSALTDRYAMVAASIRAAIDTAEDAGDMDTVDLLSGISLGMDKWLWFLEA
                     HLQG"
     gene            3168..4196
                     /locus_tag="VFQ23_17460"
     CDS             3168..4196
                     /locus_tag="VFQ23_17460"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9908441.1"
                     /translation="MTNSIFAVTTRGLEAVSADELASLPGVTVERSAYRRVFASYEGL
                     PDALLSLRTVDDLFLYVDTWQGIDRARQTLTVLQYFSAHLDLDAALAVCGQVRTIQNP
                     PIFSITANFVGKRNYNTDEIKRACASGITESYGWAYSEDDSNADLNVRVFIEGETAVV
                     GLRLAARSLSKRAYKREHVPGSLKPTVAAALLRLANVTSTARILDPCCGAGTILIEAK
                     SYGAMAWGGDINQQAITAARLNATQAGADVDLQQWDARALPISDRSVDCVISNLPWGR
                     AVTTNSSLSGLYQEISEEIERVLTPGGRVVLLTNLPDLLQFKHLNCDKQMEISLFGQT
                     PTITVWSD"
     gene            complement(4349..>4552)
                     /locus_tag="VFQ23_17465"
     CDS             complement(4349..>4552)
                     /locus_tag="VFQ23_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908442.1"
                     /translation="FHAAINTTLGTLGVLGQADGDVRSLIINTVLTWAVVGVVVAVFG
                     SDLKSTRRDRMAKEKMDSRLRHV"
BASE COUNT         1223 a         1067 c         1192 g         1070 t
ORIGIN      
        1 atgtgagata tcgagaccac aagttaacag aaaaatttat gtaacctttg gtctcgatac
       61 gtcctgcgag gatcacgcag gactactcga ccaacgagtc tgtaggaaag gagacacatg
      121 ttacgaagga ttctttcatt cagcatgata agtttagcca ttatgggagc agcctgtagt
      181 cgtcttactc aatcgcccag ttcagtcatt gcaacgacac cgaaagttgt agaaaagcat
      241 ctttctgcct tccgccccat cgagggcacg agttatatga tcgcggatat ttccggaaat
      301 ccccaggtag aggaacgagg cagtttctca ccatttcgct ggatcgatag cggttattcc
      361 ggatattccg gctatgagat ttataacttt gtctttttca acagtcagac agaaacctac
      421 aacagattaa tgttgaccaa tgaatttgtt gtccttcaaa ttgctggctt tccgcctggc
      481 acacccacgg aaaatcctgc agatttcaaa cccgtgatgt ggtggatgta tgttttagcc
      541 aaagccgata cggatcagaa cggtgttttg aattatcagg acaaattgac gataggcgtt
      601 acagatgtgg gtgggaacag ccctaccgag ttaattccca acgtagatag tgtgctgggt
      661 caaattttga aagaggacga tacactcttt gtgatctatc attctcttga taagaattac
      721 attgccaaaa ttgacctgcc gggtcgcctg gtaattagta cagccgagat gagtatcggt
      781 gaagacgtaa agtgagcctg acatgggatt tctaaaaaaa ctgttcgggg gcacacctgc
      841 caaatcaaat aagcgatatt atgttttcca ggtacgttgc aagcgctgtg gagagctcat
      901 cgagggccgc gttgacctgg ataatgatct gagtgtcgaa ttcgaagagg atcgcgaagt
      961 cttccacgtt cgcaagggat tggtcgggaa caatcgctgt tttcagcaga ttgaggtgga
     1021 attaaaattc gactcagagc gtcaattaat agaacaccaa gtgcagggtg gaacatttat
     1081 agaatagact atggaccata gacaatcgac gatgagctca tggtcaatag tctatggtcc
     1141 atggtccgag tcgaaaggaa attatgcgct acacatcccc tcccaatcaa caagcctatt
     1201 atgaacaggt ctggaatctt gtgcgtcaga ttccaagcgg taaggtggct tcgtatgggc
     1261 agatcgcgct aatgattcct ccaccggtgg atgtggagtt tgaatcatat aaggcattca
     1321 gtccgcgctg ggtgggtgga gcaatggcgg cttgccctga tgacgtaccc tggcagcgtg
     1381 tgatcaactc gcagggaaag atcagcgagc gcgccggggc ggagaaacag cgtcaattgt
     1441 tagagcagga aggggtggaa ttcgacgcca aagaccgagt cgattttaag aaatatgggt
     1501 ggaaaggtcc aaatgaagaa gatcaaccgc agcagccaag tttgttttaa acccccctta
     1561 agctaacgcc ttcccaaagg tgctatactt ttaatataga ttcattcgca atcgaaccta
     1621 taatttattt gtacgcaaac cacagacagg atggttttta tggatattta tcttgcaact
     1681 gaacatgctt atcactttat cccgcagttc aatgtggaag tggcccgcga tcgcgtggaa
     1741 cagaaaaaag taaatctgat cgcaggcact ctgggatccc tgttctcacg cgccaagccg
     1801 gaagatctgc agttggcaac cgtcgaaaac cgcgtcgaac cattctggct gttggaagcc
     1861 tcctctcgca ccgtctatga tcgggcgcgc acctacacta ttccgtctgg gggcgcggaa
     1921 gtgagaagtg tcaccgtgat gggcactgaa tttcctgtcg tgacacaacc gaagggtgac
     1981 aatgccttca cggtcacagg ggttgaacac tgtgtgcagg aactcaaaag ccgccagacg
     2041 tttgatggca tcaccggcgc caaaacggac ctcctgaaat atgcaggttc cgcaaaaaca
     2101 gagatcgcgg acctgaccaa cttcgcgccg gaaggtgtgc tggtcgtgcc ccctcaggtc
     2161 aaggcgaatg ccgtggtcag gcaggtcacc gcggatgtcg tccagccggt tcagaatgtg
     2221 caaacgattc acgaggaaag agtggacgtt gaaacgattg acttgaattt ccgccctatt
     2281 tatgcgttcg aatatgaatg ggccgcgaaa aacaaaaggg tggtgatcga gtttgatccc
     2341 cttactggcg aattgcatac gggtgggaaa cgatggagtg accagatcaa aagcatggtc
     2401 acgcgagacc tgatctttga tatcacggcc gacgcctttg gcacgattgt tcccggaggc
     2461 agcatcgcag ttaagctggt caaggctgtg gtggaccgta aaaaataagt gactgtttca
     2521 cgttttggtc gttaatctat atgagaaagc aaataacacg agaaatatag attaataaaa
     2581 aggaagaaca ggtatgaaga ccaaaacgaa aatgctgaac acaaataatg atctccctgc
     2641 caatactcgt gaacaaatca tcaagttgtt aaaccaggaa ttggcagata tgtctgactt
     2701 gcacagtcag accaaacaag cccattggaa tgtgaagggc gagcatttca tctcgctgca
     2761 caagttgttt gacgaattcg ccgaggccct cacaggttac atcgatgaaa tcgccgaaag
     2821 agccgtacaa ctcggcggca ccgccatggg gacaaatcgc atgtcggcgg cagaatcgcg
     2881 actaccagaa tatccgtcag aagtcatggg cagcatggat cacgtcagcg cgttgaccga
     2941 ccgctacgcg atggttgcgg caagcatccg cgccgccatt gacacagcgg aggacgctgg
     3001 cgatatggac accgttgatc ttctcagtgg catctcgctt ggcatggaca agtggttgtg
     3061 gttcctggaa gctcacttac aaggctgagt gagacaaatt ccgtaaataa aaagctacga
     3121 tcttgtggct caacacaacg caggtgggtg tatcatagac aaattacatg acgaacagca
     3181 tctttgccgt gacaacccgc gggctggagg cagtcagtgc ggatgaactt gcctctctgc
     3241 ccggggtaac cgtggagcgt agcgcctatc gacgcgtctt tgcctcgtat gagggactac
     3301 ccgatgctct gctttccctg cgaacggtag atgacctgtt tctttatgta gatacctggc
     3361 agggaattga tcgcgcacga cagacgctaa ctgtcttgca gtattttagt gctcacttgg
     3421 atttggatgc cgccttggca gtctgtgggc aagtccgtac gattcaaaat ccgcccatct
     3481 tttcaatcac agccaatttt gtcggcaagc gaaattacaa caccgatgaa atcaaacgag
     3541 cctgcgcttc agggatcact gagtcatacg gttgggcata ttcagaagac gactcaaatg
     3601 ccgacttgaa cgtgcgcgtc ttcatcgagg gtgaaacagc tgtggttggg ctccgtctgg
     3661 cagcgcggtc cctgtctaag cgcgcctaca agcgggagca tgtgcccggg tcattgaagc
     3721 ctacggtagc cgctgcgctg ttaaggttgg cgaatgtgac ttccactgcg cgaattctgg
     3781 atccgtgctg cggcgccggg acgattctga tcgaagcaaa atcctacgga gcgatggcct
     3841 ggggtggaga tattaatcaa caagccatta cagcagcgcg cctcaatgca actcaagcgg
     3901 gagcggatgt ggatcttcag caatgggatg cacgtgcgct accgatttca gaccgatccg
     3961 ttgattgtgt aatttcaaat ttgccatggg ggcgcgctgt caccacaaat agctcgcttt
     4021 ctggtctcta ccaggaaatc agtgaagaga tagaaagagt tctcacgcca ggcggacgag
     4081 tagttctgct caccaacttg ccagacttgc tgcaattcaa gcacctcaac tgcgataagc
     4141 agatggagat cagtctgttt ggacaaacgc ctacgattac agtctggtct gattgacctt
     4201 aaggcgtttc acgcctgacg tggaaagctg gagtggaatg aaacatcgcc atgaagcgat
     4261 agagtccatt tgaagatgta tcgatagagc tttggcaaaa ttccaggatg agtaaggagt
     4321 agataaaacc atatgggtat gcctggagtc aaacatgacg caggcgtgaa tccatttttt
     4381 ctttcgccat cctatcccgg cgcgtcgact tcaagtcgct tccaaaaact gcgacgacga
     4441 ctccaaccac ggcccacgtc aaaactgtgt tgataattaa cgatcgtaca tcaccgtcgg
     4501 cttgacccag cacgcccagc gttcccagag tagtgttgat cgcagcatgg aa
//
LOCUS       DASTJP010000593         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_680333, whole genome shotgun sequence.
ACCESSION   DASTJP010000593 DASTJP010000000
VERSION     DASTJP010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680333"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VFQ23_17470"
     CDS             <1..724
                     /locus_tag="VFQ23_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9908443.1"
                     /translation="FMVDPADIRKALTPRTKVIFIGYPSNPTGAVATREVMLEIAKIA
                     EEHDLLVVSDEIYDRLVYDFEHVCFPGLDESLKHRTILLGGFSKDYAMTGWRIGYACG
                     PSDLIQGLVRIHQYSIMSSPTTAQDAALEALRSGDPYVEEMRLEYDRRRRLLVAGLNR
                     LGLSTFEPRGAFYAFPNIRASGMDDETFAEKLLVEEHVAVVPGNAFGPGGNGFVRMCY
                     ATAYEQIEEALRRMEKFMNRYG"
     gene            909..1487
                     /gene="infC"
                     /locus_tag="VFQ23_17475"
     CDS             909..1487
                     /gene="infC"
                     /locus_tag="VFQ23_17475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET9908444.1"
                     /translation="MSATEFRVNEGIRVPEVRLIGPNGENIGVVPMKQALQIARDADM
                     DVVEVSPNATPPVCRVMDFGKFIYERAKKEREAKKSQTKIEVKEIRLRPKTNEAHRGF
                     KVDDARRWLLHGNKVRVTIKFRGREMDYPEIALEDLREIAEALSDVSTVEQAPQMEGR
                     TMLVVLAPSKGGAKKKEKGEPAQVQKEPQAQQ"
     gene            1597..1827
                     /locus_tag="VFQ23_17480"
     CDS             1597..1827
                     /locus_tag="VFQ23_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HET9908445.1"
                     /translation="MPRKAKAGKTKLKTHKATSKRFRLTGSGKVVRTKGGKSHLRRRT
                     SKRTKAQLSEMHEVKGRKIIKRIKRLAPNMEG"
     gene            1954..2295
                     /gene="rplT"
                     /locus_tag="VFQ23_17485"
     CDS             1954..2295
                     /gene="rplT"
                     /locus_tag="VFQ23_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET9908446.1"
                     /translation="MRVKGGPQGHYRHKRVLKITKGQRGAKHRLFKRANEAMLKSLWY
                     ATRDRRVRKRDLRKLWIARINAAARLNGTTYSKLVAALKKANIDLNRKMLSDIAVRDP
                     QTFAAVVAKTK"
     gene            2482..2721
                     /locus_tag="VFQ23_17490"
     CDS             2482..2721
                     /locus_tag="VFQ23_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908447.1"
                     /translation="MPSQNFPGLLLQGETLSTLLSLATSTYEKSQKTSDTELVDASRE
                     LRDNLQNLVSHYEATLRKHNIPLPYSTVPLTRHEP"
     gene            2752..3315
                     /locus_tag="VFQ23_17495"
     CDS             2752..3315
                     /locus_tag="VFQ23_17495"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET9908448.1"
                     /translation="MNTVLVERRLPSNQTVQIVQGDITSEEVDAIVNAANEHLQHGGG
                     VAWAISRKGGAAIQKESNDWIRQHGRVSHANPAWTSGGLLPARYVIHAVGPVWGDGDE
                     DHKLEAAVTGALRAADELYLTSIAMPAISTGIFGFPKDRAAGIIYTAIEAYFIEHTSS
                     ALNTVKLVLFDQKTVDIFIKAWQDRWG"
     gene            3321..>4050
                     /locus_tag="VFQ23_17500"
     CDS             3321..>4050
                     /locus_tag="VFQ23_17500"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9908449.1"
                     /translation="MITSSQNSKIKLVRALFGRAKERREAEAFVVEGVRLVDEAVNSN
                     WGFKFALYSDDLSEHGKQVAEKLQNKNIDVEKVESNLFRKLSGTESPQGILAVINDSQ
                     LPFPDPANFILIPDQIRDPGNLGTLLRTAAATGVDAVLLPPETTDAFAPKVVRSGMGA
                     HFQLPIHPMTWDEIRERTQNVQVYLADMDGKSYWETDLSQSLALIIGSEADGASAEAQ
                     NLATQKISIPMAKNVESLNAGVAGS"
BASE COUNT         1116 a          975 c         1043 g          916 t
ORIGIN      
        1 tttcatggtc gaccctgccg atatccgcaa ggcgcttacc ccgcgcacaa aagtgatctt
       61 catcgggtat ccttccaacc ccaccggtgc cgtggcgacg cgtgaagtga tgctcgagat
      121 cgctaagatc gcggaagaac atgatttgtt ggttgtctcc gatgaaatct atgatcgtct
      181 cgtctacgac tttgagcacg tctgtttccc cgggctggat gaaagcctga aacatcgaac
      241 gattttgctt ggcggtttct ccaaagatta cgccatgaca ggctggcgca tcggctatgc
      301 ctgtggacca tcggatttga tccagggact ggtgcgcatt catcaataca gcattatgtc
      361 ctcgccgact accgcgcagg atgcagcgct ggaagcattg agaagcggtg atccttatgt
      421 ggaagagatg cgcctggaat atgaccgccg ccgccgattg ctcgtcgcgg gacttaaccg
      481 actcggtctt tcgacgtttg agccgcgcgg cgcgttttac gcattcccca acatccgggc
      541 ctccggcatg gacgatgaga ccttcgccga gaaactactg gtcgaagaac acgttgcagt
      601 ggtaccaggt aatgcgttcg gtcccggtgg caatggcttc gtgcgcatgt gctacgcaac
      661 agcctacgaa cagatcgaag aagccttgcg ccgcatggaa aagtttatga acaggtatgg
      721 gtaactatga atagaagtaa caggattgcc agaaaagctc gagagtcgta aaatacttgt
      781 agaccgtggg cgacggagga tttgatcgtc tacggtctat tttattgacg attctgcaac
      841 ctacgtggta ttattcgccc actcaaaacc agcccttggc tggatttatc atgttatagg
      901 agaaacttat cagcgctaca gaatttcgag taaatgaagg cattcgggtt cccgaggtcc
      961 gtttgattgg tcccaatggc gaaaacatcg gtgttgtgcc aatgaaacag gccctgcaaa
     1021 ttgcccgcga tgctgacatg gatgttgtgg aagtctcacc aaacgccact ccccccgttt
     1081 gccgcgtaat ggattttggc aagtttattt atgagcgtgc caaaaaggaa cgcgaagcca
     1141 aaaagtcgca aacgaagatt gaagtaaaag aaatccgcct gcgccccaaa acgaacgaag
     1201 cacaccgcgg tttcaaagtg gacgatgccc gacgctggtt attgcacggg aacaaagtac
     1261 gtgtgacgat caagttccgt ggccgtgaaa tggattaccc cgagatcgct ctcgaagatt
     1321 tgagagagat cgccgaggcc ctttcggatg tcagtacggt agaacaggcg ccacaaatgg
     1381 aagggcgcac gatgctcgtg gtgctggcac ccagcaaagg tggcgcaaag aagaaagaaa
     1441 agggcgaacc ggcccaggtc cagaaagagc cgcaagccca acaatagacg cctcgagtcg
     1501 tctttccagt aagtcccaaa gatgttgaag tccgcgggtg agaaactacc ccgcggtttt
     1561 tttacgccga aaattgtttg aaaggagcaa gcaacaatgc ctcgcaaagc caaagctggc
     1621 aaaaccaagc taaagaccca taaggcaacg tccaagcgct ttcgcctgac gggttccggc
     1681 aaggtcgtgc gaaccaaagg cggcaagagc cacctgcgca gacgcacatc caagcgcaca
     1741 aaggcacaat tgtctgaaat gcacgaagta aaaggccgca agatcatcaa gcgcatcaag
     1801 cgccttgcgc cgaacatgga aggttaagga catccagctg gttgtcctta caatccggtt
     1861 atcgtatcca atttttagtt gcggctttcg ggtgttcgca actggctctg ccaatagcag
     1921 gccgacgccc gcgagtttgc aggagagtag acaatgcgcg ttaagggagg ccctcagggc
     1981 cattatcgtc acaagagagt tttaaagatc acaaaggggc agcgtggtgc aaagcatcgc
     2041 ctgttcaagc gtgcaaatga ggctatgctg aaaagcctat ggtacgcaac tcgtgatcgc
     2101 cgtgtgcgca aacgtgactt gcgcaaatta tggatcgctc gcatcaatgc ggcagcccgt
     2161 ttgaatggaa ccacctacag caagctggtg gctgcgttga agaaagccaa tattgatctc
     2221 aatcgcaaaa tgctatctga tatcgcagtc cgtgacccgc agacatttgc tgcggtggtt
     2281 gcgaagacaa agtaacaaat cgttttctcg ttagcgcgtc ggggcaggtc taagacctgc
     2341 cctgccttgt ttaaaggagt aaaataacat ttgttatgct aaatcaaaaa cttatcattt
     2401 aatgattcaa gaaagtgtgc aaacatggaa tacatggaat tagaagttta ttctcaatca
     2461 atcaatcggg gtatcgttaa aatgccaagc caaaactttc cgggcttgct cctgcaaggc
     2521 gagacgttgt caactttgct gagtctagcc acatcaacct atgaaaaatc gcaaaaaaca
     2581 tcggataccg aattggttga tgcttcacgc gaattaagag acaaccttca aaacctggtt
     2641 tcccattatg aagcaacgct ccgcaagcat aatattcccc tcccgtattc cacggttccg
     2701 cttacacgcc atgaaccgta aggcaaatcc tcttaaaaac ccatccagct tatgaataca
     2761 gttcttgttg aaagaaggct tccgtcaaat caaaccgtcc agatcgttca aggtgacatc
     2821 acaagcgaag aggtggatgc aatcgtcaac gcggcgaacg agcatttgca gcatggcggt
     2881 ggcgttgcct gggcgatatc aagaaaaggt ggagccgcga ttcaaaaaga atccaatgat
     2941 tggattcgac agcatggacg cgtctcccat gcgaatcccg cgtggacttc tggcgggctt
     3001 ctgcctgcga gatatgtgat ccatgctgta ggtcccgtct ggggagatgg agatgaggat
     3061 cataagcttg aagcggctgt gaccggtgca ttgcgcgccg ctgacgaatt gtatttgaca
     3121 tcgattgcca tgccggccat atcaacaggc atttttggtt ttccaaagga tcgggccgct
     3181 ggaatcatat atacagccat tgaagcgtat tttatcgaac atacatcatc cgccttgaat
     3241 actgtcaaac tcgtcttgtt cgatcaaaag accgtggata tctttatcaa agcctggcaa
     3301 gacagatggg gctagcctga ttgattacct cctctcaaaa ttcaaagatc aaactggtac
     3361 gcgccttatt cggacgtgca aaagagcgac gtgaggcaga ggcttttgtg gtggaaggtg
     3421 ttcgattagt tgacgaagcc gtcaatagta attggggttt taaatttgcg ctctacagtg
     3481 atgatctgag tgaacatgga aaacaagtcg cagagaaatt acaaaataaa aacatcgacg
     3541 tagagaaagt ggaaagtaat ttattcagga aattgagcgg tacagaatct cctcaaggca
     3601 ttctcgctgt gataaacgat tcccagcttc cttttcctga tcccgcaaac ttcattctca
     3661 tccccgacca aatccgtgac ccgggcaacc tgggtacgtt gctccgcacc gcggcagcaa
     3721 ccggagtaga tgctgtattg ttacctccag aaacaaccga tgctttcgcg ccaaaggtag
     3781 ttcgttcagg aatgggagca cattttcaat tgccaatcca tccgatgaca tgggatgaaa
     3841 tacgagaacg aacacagaat gtacaagtgt atctcgcaga tatggatgga aaatcgtatt
     3901 gggagaccga tttgagtcaa tcacttgcat tgatcattgg cagcgaagcg gacggtgcga
     3961 gtgcggaagc tcaaaacctg gcaacacaga aaatatcgat ccccatggca aagaacgttg
     4021 aatcgttaaa tgcaggcgta gcggggtcag
//
LOCUS       DASTJP010000594         7220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_192291, whole genome shotgun sequence.
ACCESSION   DASTJP010000594 DASTJP010000000
VERSION     DASTJP010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192291"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFQ23_17505"
     CDS             complement(<1..287)
                     /locus_tag="VFQ23_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908450.1"
                     /translation="MKNSFRQIIIVLTLLTALACSGLPGVSSTPATEPEYRYDDLPAP
                     VNEDSIISKYRAISQWSKLDINYFFVNDTTKLEGNTERELVRQAFGLWAQET"
     gene            442..909
                     /locus_tag="VFQ23_17510"
     CDS             442..909
                     /locus_tag="VFQ23_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908451.1"
                     /translation="MNSPMVITSNDSILRTLDFRPYRSKVERRVIPFLPRPGEPQTLE
                     VSTPWGAHLTAKYGDFLVSEVDTPNDFWPIDPVIFEESYVIIRPGYCVKKAITLLVPL
                     TDITHDPNRQVTVISLEGPETVRAGDFYLAKGIKGEVWPYPKDKINDVMLPAD"
     gene            complement(925..3273)
                     /locus_tag="VFQ23_17515"
     CDS             complement(925..3273)
                     /locus_tag="VFQ23_17515"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HET9908452.1"
                     /translation="METITTSPEIQEETPIVGPVAAGSEHTESQVKILEMAEENDKGS
                     TSIRPEIRPHAFVIMPFGKKKGADGKPYDFNAIYEQLIKPVLEQAGFEAFRADEENTS
                     GDILTDMFQELLLADLCIADMSIDNANVFYELGIRHAFRKRGVVHIQAGRAYMPFDVF
                     NVRTIPYHITDEGLPDPKFLEKDKAVIIRGCRATWASEPERVHSPIYNLLTGLVEPER
                     RTLRTPLATGFWREYYEWKQRVTIAQRQKRIGDILLLTEEIRNPLIKEEAIGEAGKAL
                     ANMGRNELALNQYRKGLEVNPRNLTFRREEAFHLNRLGRVDDAIVKIEGILSDVATDF
                     ETVAYLGRIYKDMWTESWMWIRERDLRLKTAFESYHWLIKAFETYLKGYRIDLDQSNT
                     TPGVNALTLGTILIYLADKFDNQTQPDPEITWVRELLPDLRGSLLFGLESKAQLDGAD
                     YWTLVSLAELRVLTAEVTQQVTRAYRKALTATRRNLFFLQSSLRQLEVLHSLNMRSEF
                     VHAGITAIKEEIKRIQKDVLTEKPSKGKADKVEKPKKGSGLVFIFTGYMVNHPKKKED
                     HFPPEKEPEIKAAIGTVLDKYGAGPSDLAVSTGMDAGSEILFVECCVERGIPVQAYFP
                     MSEAPYIRDFVSPCGGKWVERFYTMRNDPLVTEYYQPDSVGLPKEGDNVHERNNRWAL
                     YSALSRDIDKMRLIAVWDGKNESSKDLDARLVKHMVDLMRETGGIVEQINPGKLSRDV
                     VEVMTVSDNVHFSLKLKANSSDKAATPKPTQQKRKPSSKTTG"
     gene            complement(3325..4704)
                     /locus_tag="VFQ23_17520"
     CDS             complement(3325..4704)
                     /locus_tag="VFQ23_17520"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HET9908453.1"
                     /translation="METEATKNTPILNIAWTRFAQLDASSKRGTKLYRDIRRWIAALG
                     VLATVFALLSLVFPREGSGIPRPGFITGFCGEDSFCNVIGLGTRVLLVTIPILASALA
                     AIATRGFSNGDWLISRAGAEEIQKEIYFYRTILQKKKSRRVYLEKRLNEIQRQIYQGM
                     NGEFSFEPYKGPIPPNYSASDPSSDPGFQDLTGEEYFNYRLDYQLNWHNRKINQYKAE
                     RFRLQFYIIIFGAAGALLAAFDSTTILVALTASLTAALVGWQEIRNLDSIIRNYSKVV
                     MELTILHDHWKNLELEERTDAEFYKIVQGCEDVLWAQNTEYIKSMQEALKDSDLEREA
                     SLINRVIKESVESDARIKEDIRNSVVDLTGKTLAEAELTVEEEYQSALGALAEEASSE
                     LVQQELAAMRQAAAEMAENVAERASNLMSSIKDIVKDIADGEISRDTSSKDLNEILSR
                     IPKTQDVKG"
     gene            complement(4753..6141)
                     /locus_tag="VFQ23_17525"
     CDS             complement(4753..6141)
                     /locus_tag="VFQ23_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908454.1"
                     /translation="MFKTQNLYQNDEKWKNTKLGNSSETIGGWGCLLTSVTMMLNGIG
                     YNETPESVNQKMKGAGGFQGAFFIPSVLPYVWPNCAYRDMQPCEGSVPVSMIDSAVAA
                     GKPVILQVDWNKQAGIQTHFVLVKEKKGNDYVIYDPYKYGGDGPDKEVLLTSRYKYNG
                     AVIEKEISGVLWFDSYSSSPPEPPKVNKVPLPAEKYTLYAAEDDLALRADPSVGGYLW
                     KRMTMGTELTSLEATKAAAQAKLGKNGEWIKVQDPKGDQGFVAAWYVSDTKGKPASAT
                     SAATTAKPVSTVSSTTASVPPGALAFIPTEQLSLRSRPEISPQTLIRYVPVTEQLVSL
                     EPADVVIPKVGVSGQWLKVRDAANKEGYAAAWYVKYAGGSTAQIAAATTSSTPVSGGP
                     IRVRATAEAVAFRKQPIINESNVIRRVSIGTEFMVAEPNGETKIGKNDQWLKVKDSTG
                     TEGYLAAWFVSR"
     gene            complement(6388..7074)
                     /locus_tag="VFQ23_17530"
     CDS             complement(6388..7074)
                     /locus_tag="VFQ23_17530"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9908455.1"
                     /translation="MTTRLYLVRHGATQLTAEDRFSGAANVYLSEEGQSQAAHLANRL
                     VDDDISAIYASPLDRTMETAAILAKPHNLTVNSYDGLREISHGHWEGLTRKEVEERFP
                     EEYTAWESDPFTFAPPGGESGISVLARALPVIREIVVNHRDKNVLVVSHKATLRLIIS
                     SLLGFDARGYRDRLDQAPACLNILDFKDTVRARLMLFNDISHYADHPHRPQKHLSRWW
                     DPASISDNSA"
BASE COUNT         1677 a         1785 c         1743 g         2015 t
ORIGIN      
        1 gtttcctgtg cccacaagcc aaaggcttgt ctgactagct ctcgttccgt gttgccttcc
       61 aatttcgttg tgtcgttgac gaaaaagtaa ttgatatcaa gcttgctcca ctgcgaaatg
      121 gcgcggtact tggaaatgat actgtcctca ttgacggggg cgggaagatc atcgtaacga
      181 tattccggct ctgttgccgg agtagaagaa acacctggca gcccggagca ggccagagca
      241 gtgagcagcg ttaacacgat gataatttga cgaaaggaat ttttcatact tcgattctcc
      301 aggaggtttt gctatacggg catattgtct tttatagcat acgcgagttt gccttacaag
      361 gcatctgttt gaggcataga tgtcgcgaac ttgcaacaaa tctcttccag ttccatatgg
      421 tagaatcaat aaacgatttt tatgaactca cctatggtca taacgtcgaa tgattcgatt
      481 ttgcggaccc tggatttccg cccgtatcga agcaaggttg agcgacgggt tatcccattt
      541 ctacctcgcc ctggcgagcc tcaaacattg gaggtcagta caccctgggg cgctcacttg
      601 acagcaaagt atggggattt tcttgttagc gaggtggata cacccaacga tttttggcca
      661 attgaccctg tgatctttga agaaagttat gtcattatcc gcccgggata ttgcgtgaaa
      721 aaagcgatta cccttctcgt gcccttgacc gatatcactc atgatccaaa tcgtcaggtc
      781 accgtgatta gtttggaggg acctgaaacg gtgcgagccg gggattttta tcttgcaaaa
      841 ggaatcaaag gcgaggtctg gccgtacccg aaagacaaga tcaacgacgt gatgctgccc
      901 gctgattagt cagcaggcag ttttttatcc cgtagttttt gaggagggct ttctcttttg
      961 ctgggttggt tttggcgttg cggctttatc tgatgaattt gccttaagct tcaaactaaa
     1021 atgaacatta tcgctcacgg tcattacttc aacaacatcg cgggataact tgccaggatt
     1081 gatttgttct acaattccac ctgtttcacg catcaagtca accatatgtt ttaccagccg
     1141 ggcatcaaga tcttttgatg attcgttttt tccatcccaa acggcgatca ggcgcatctt
     1201 gtcgatgtca cgcgagaggg ctgaatacaa cgcccaacga ttattgcgct catgtacgtt
     1261 atcaccctcc ttgggtaacc ctacactatc gggttgataa tattcggtta cgaggggatc
     1321 attgcgcatg gtataaaagc gttccaccca ttttccgccg catgggctga cgaaatcccg
     1381 tatataaggc gcttcagaca tcgggaaata ggcctggact ggaatgccgc gctctacaca
     1441 acactctaca aagagaatct cgctgcctgc gtccatcccg gtcgaaacgg ccaaatcact
     1501 gggaccggcg ccgtatttat cgagtacggt gccgatggcg gccttgatct ctggctcttt
     1561 ttccggcggg aagtgatcct ctttcttttt gggatgattg accatataac cagtgaaaat
     1621 aaatacgaga ccacttccct ttttcggttt ttcgacttta tcagccttgc ctttactggg
     1681 tttttcggtc agtacatctt tttggatgcg tttgatttct tccttgattg cagtaatgcc
     1741 agcgtgcaca aattcggagc gcatgttcaa tgaatgcaat acttccaatt gtcgcagcga
     1801 agactgcaga aagaacaggt ttcgccgtgt ggctgtgagt gccttgcgat acgcacgcgt
     1861 cacttgctgt gtaacctccg cggtaagcac acgtaattct gccagtgaga ccagagtcca
     1921 atagtctgca ccatccagct gtgcctttga ttcaagaccg aataagagcg atccacgcag
     1981 atcaggcagc aattcacgca cccaggtaat ttctggatca ggttgagtct ggttatcaaa
     2041 tttgtctgca agatagataa ggatcgtccc aagggttaga gcattgacgc caggggttgt
     2101 attgctttga tccaggtcaa tacgatagcc ttttagatac gtttcaaatg ctttaatcag
     2161 ccaatgatag gactcaaagg ctgtcttaag acgcaaatct cgttcgcgaa tccacatcca
     2221 ggattcggtc cacatatcct tgtaaatgcg cccaaggtag gcgacagttt cgaagtcagt
     2281 tgccacatca gacaggatgc cttcgatttt gacgatggca tcatccacgc ggccaagtcg
     2341 gttcaaatgg aaggcttcct cgcgtcggaa agttaaatta cgtgggttta cttccaaccc
     2401 tttccgatac tggtttaatg ccagctcgtt gcgtcccata ttggcgagag ctttgcccgc
     2461 ctcgccgatg gcctcttcct tgatcagcgg atttctgatt tcttcggtca gcaacaggat
     2521 atcgccaatg cgcttctggc gctgtgcaat cgtcactcgt tgcttccatt cgtaatattc
     2581 gcgccagaag cccgttgcca ggggagtgcg caacgtcctc cgctccggtt caaccagccc
     2641 ggttaataag ttgtaaattg gactatgaac gcgttcaggc tccgatgccc aggtggcgcg
     2701 gcacccacga ataatgacgg ccttgtcttt ttctaagaac tttgggtccg gtaatccttc
     2761 gtcagtaatg tgataaggaa tcgtgcgcac attgaacaca tcaaatggca tgtacgcgcg
     2821 ccccgcctga atatgcacca cgccccgttt acggaaggca tgacgaatgc ctaattcata
     2881 gaatacattg gcgttgtcga tgctcatgtc cgcaatgcac agatcggcca gcaacaattc
     2941 ctgaaacata tcggtcagaa tgtcgccgct ggtgttttct tcatcggcgc ggaacgcctc
     3001 aaagccagcc tgctctaaaa ccggcttgat caactgctcg tagatggcat taaaatcata
     3061 gggttttcca tctgcgcctt tcttttttcc aaacggcata attacaaatg catggggtcg
     3121 tatttcaggt cgaatggaag tagatccctt atcgttctcc tcggccattt caagaatttt
     3181 tacctgagat tccgtgtgtt cagagcccgc ggcaactggt cccacgatcg gcgtttcctc
     3241 ttggatttcc ggagatgttg ttatggtttc catgacatat tcttccttct cacctgattt
     3301 gttgtgcaga gcaacagttt ggtttcaacc ctttacatcc tgtgttttgg ggatgcggga
     3361 caggatttca ttgaggtcct tgctgctcgt atccctactg atttcaccat ccgcgatgtc
     3421 tttgacaata tctttgatgg agctcatcag gttcgacgct ctttcagcga cattttctgc
     3481 catttctgca gccgcctggc gcatggcagc cagttcctgc tggacgagtt cagaagacgc
     3541 ctcttcagca agcgcgccga gtgcggattg gtactcttct tccactgtta attctgcctc
     3601 tgccagggtt tttccggtta agtccacaac actattgcgg atgtcctctt tgatgcgtgc
     3661 atcagactca actgactctt tgatcactcg attgatgagg ctggcttctc tctcaagatc
     3721 agaatcttta agtgcctctt gcattgattt gatgtattct gtgttttggg cccataatac
     3781 atcctcgcag ccctgtacaa ttttatagaa ctcggcgtcg gtccgttctt ccagctccag
     3841 gtttttccaa tgatcatgga ggatcgttag ttccatcaca accttgctgt agttcctgat
     3901 gatcgaatcg aggttacgga tttcctgcca accgacaagt gctgctgtga gtgaagccgt
     3961 cagtgcaacc agaatggtcg tagaatcgaa tgctgccagt aaagcgcctg cagcaccaaa
     4021 tataatgata tagaattgca gacggaatcg ctcagccttg tactgattta tcttcctgtt
     4081 gtgccagttc agttggtaat cgagcctgta gttgaagtat tcttcaccag tcagatcctg
     4141 aaagcccggg tcagaagagg gatcgcttgc agaataattc ggaggaatgg gacctttgta
     4201 tggctcaaag gaaaactcac cgttcattcc ctggtagatt tgtctttgaa tttcattgag
     4261 ccttttttcc aaataaacac ggcgggattt tttcttctgt aatatcgtgc ggtagaaata
     4321 aatttccttt tgaatttcct ctgcaccagc gcgtgagatc agccagtcgc cgttggagaa
     4381 gcctctggtg gcaatggcgg ctaatgccga agccaggatc gggattgtga ccagcagaac
     4441 tctcgtacct aaaccgatta cgttacagaa actgtcctcg ccacagaaac ctgtaataaa
     4501 ccctggtctt ggtatcccag aaccttcgcg cgggaatact aaggaaagga gggcaaaaac
     4561 tgttgctaga acgcccagtg cggcaatcca tcgcctgata tctcgataaa gctttgttcc
     4621 tcttttcgaa gatgcatcca attgtgcaaa gcgcgtccat gcgatattga ggatcggtgt
     4681 gttttttgtt gcttcggttt ccatgaagag ctcctccgac attaagccaa ttgataagta
     4741 aaggatgttt atttagcgcg agacaaacca ggcggcaaga taaccctctg tgccagtgga
     4801 gtctttaact tttagccatt gatcgttttt cccaatcttt gtttctccat ttggttcagc
     4861 aaccataaat tcggtgccga tggagacgcg tcggatgaca ttgctttcgt tgataatagg
     4921 ctgtttacga aacgcgaccg cctctgccgt tgctcttact cttattgggc cgccagagac
     4981 tggcgtcgag gaagttgtgg cagctgcgat ttgcgcagta gagccgcctg catattttac
     5041 gtaccaggcg gcagcgtatc cttctttgtt tgcggcatcc cgcactttga gccactgccc
     5101 gctgactccg actttgggta tgaccacatc ggctggctca agagatacca attgctcagt
     5161 aacgggcaca tatcgaatca gcgtctgcgg cgaaatctct gggcgggaac ggagggagag
     5221 ttgttcggtg ggaataaaag ccaatgcgcc cggaggtaca gatgcagtag tggaggagac
     5281 ggtagaaacg ggcttggctg tcgttgcggc cgatgtagcc gaagcaggct ttcctttcgt
     5341 atccgagacg taccaggcgg ctacaaatcc ctgatcccct tttggatcct ggacttttat
     5401 ccattcaccg tttttcccga gtttggcctg ggcagcagct tttgtggctt caagggaggt
     5461 gagttcggtc cccattgtca ttcgcttcca aagatagccg cctacagatg gatccgcgcg
     5521 cagggctaag tcatcctctg ctgcatagag ggtatatttt tcggcaggga gtggcacttt
     5581 gttcactttc ggaggctcgg gcggtgagga actatatgag tcgaaccaaa ggacaccgct
     5641 gatctccttc tcaattactg cgccattata tttatagcgg cttgtcagga gcacttcctt
     5701 atccggacca tcacccccat atttatatgg atcgtagata acataatcat tccccttttt
     5761 ttccttgact aacacgaagt gagtttgaat gccagcttgc ttattccaat ccacctgaag
     5821 gataacgggc ttacccgccg cgaccgccga gtcgatcatg gaaacgggaa cggaaccttc
     5881 acaaggctgc atatcccgat atgcacaatt gggccagaca tacggcagca cgctggggat
     5941 aaagaatgcg ccctgaaaac caccagcacc tttcattttc tggttgacgc tttcgggcgt
     6001 ttcgttatag ccgatgccgt tcaacatcat ggtgacggac gttagcagac atccccatcc
     6061 accgattgtc tcagatgagt ttcctagttt cgtgttcttc cacttttcat cgttttggta
     6121 aagattttgt gtcttgaaca tgaccggcct ccgtggctga aatctattgc ttcgttttag
     6181 agggtgggac tttgatactg aggaatttat tgtactccaa atcagagtcc acccaaaatt
     6241 atacttttct tgaagtcaac cctcgactct aagcccgttt tgacacgtct tctgatttgt
     6301 tatctcactt tttcatgaaa cgacgttttt cccatctcaa aataaataac acgcgacttt
     6361 gaaaatcgtc acgtgaaacc tggcatttta agcgctatta tctgaaatac tggcagggtc
     6421 ccaccagcgc gagaggtgct tctgcgggcg gtgcggatga tctgcatagt gtgaaatatc
     6481 gttgaagagc atcaaccgtg ctcgaacggt atctttgaag tcgagaatat ttaggcaggc
     6541 tggcgcctgg tctaaccggt ctcgatagcc gcgcgcgtca aaaccaagca ggctgctgat
     6601 aatgagtcgt agcgtggctt tgtgtgagac gaccagcaca tttttatcgc gatgatttac
     6661 gacaatctca cggatcacgg gcagtgcgcg tgccagcaca ctaatccctg attcgccgcc
     6721 cggtggggca aaggtaaaag ggtcagattc ccatgctgta tattcttcag gaaaacgttc
     6781 ttccacctct ttacgtgtca atccctccca gtgaccgtga ctgatttccc gcaagccatc
     6841 ataggagttc acggtcagat tatgaggctt tgcgagaatc gcagcagttt ccatggtccg
     6901 gtccagcgga cttgcataga ttgctgagat atcgtcatcg accaggcggt ttgccaggtg
     6961 tgcggcctga ctttggcctt cctctgacag gtaaacgttc gccgcacctg aaaagcgatc
     7021 ctccgccgtc agctgcgttg caccatgccg taccaaatag agccgggtag tcacaatgta
     7081 tctgcctttg ttgttccagc atacgttatt ccagcggttc aactgctgga ataaccggaa
     7141 tagctatatt ttatgtgggt ggctgcttaa cgtaaaggtc gaagaactct atagtacgct
     7201 gcattgccgt gctgaagtaa
//
LOCUS       DASTJP010000595         8908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_213887, whole genome shotgun sequence.
ACCESSION   DASTJP010000595 DASTJP010000000
VERSION     DASTJP010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213887"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1744
                     /locus_tag="VFQ23_17535"
     CDS             <1..1744
                     /locus_tag="VFQ23_17535"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9908456.1"
                     /translation="DGKLLFAKGYGYADVENAIPVDPEQTIFRIGSVTKLFTWTAVMQ
                     LAEQGKLDLDADINTYLDFRIPDTYPQPITLTHLLTHTPGFEDRFFEVLASDVDDLVP
                     VREWLVSHMPARVRPPGLAAAYSNYGTSLAGYIVAQVSGQPYEQYIQENILNPLGMVN
                     STAQSPIPSDLLAQASVGYTYEDGAFQSFPDYLGQPAFVPAASLSASATDIARFMIAH
                     LQDGRYSDANTPVARILEEASAQQMHTTLYTPDPRLLGNAYGFFDFSDNGQRTLGHSG
                     ESHPFNTLLLLLPDQNLGVFVSYNSEGGGNVTNQHLGFQRAFFDHYYPAPAAASIQPP
                     ANFTERAARFEGSYRLTRSSYTTLEKVSTLMGGTVSISDSGDGALLFTTPWGEWRFVE
                     VDPLYFRQVDGQFSLVFREDDQGRITHMFANVTPMFAFEKLNWYETPAFNMGLLLVSL
                     VIFLSMILVALIRFIRNRRPGSDPNPAPRGARMAQGIILAISVLNLLFVVGTALWGNP
                     FPVFGVSMIYKIVLGLGVLSAALTVGALVYTVAAWKNNYWGIAGRAYYTLVTVAAVGF
                     VWFLNYWNLLGWRF"
     gene            1839..2681
                     /locus_tag="VFQ23_17540"
     CDS             1839..2681
                     /locus_tag="VFQ23_17540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9908457.1"
                     /translation="MSITLEKKQPNTTQGNDPLALLQENNQYNIWQILTIWLTGSALM
                     WIMGWVIYPALSRDLSVVEAGLLRIRLLTVGLIWQFLLSMIILYMEKGNLRLTTIRRR
                     FWLNTPISPKTGQKDRRLWWLILPLLMLMVVLELGLDPILNGTWIKLFPFLAEPPGYS
                     PAILFAPELRSLWLGAWDLLALQVVLSVFNTFLGEEFLFHGVLLPKMNGVFGKWDWAA
                     NGILFGLYHLHQPWGLPGNIVSGLLLAFVAKRYRSNWFPILLHSGQSVYFIFLILGLV
                     LGLA"
     gene            complement(2751..3161)
                     /locus_tag="VFQ23_17545"
     CDS             complement(2751..3161)
                     /locus_tag="VFQ23_17545"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9908458.1"
                     /translation="MTTLDQLNARGEGLLPGLLGIEFTGYEPGRLSSRLTLRPELLAP
                     NGYLHAATIIALADTSCGYGTFTDLPDGAQNFTTIELKSNFLGTTREGGIVCVATRVH
                     SGRTTQVWDAVVTDETSGKTIALFRCTQMILYPR"
     gene            3448..5526
                     /locus_tag="VFQ23_17550"
     CDS             3448..5526
                     /locus_tag="VFQ23_17550"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9908459.1"
                     /translation="MPRIFISYRRSDSGVFTGRIHDQLKARFGANNVFRDVYNIPAGS
                     DFRTVLNEEVGSTDVCLVMIGPQWVTTTDAQGKRRLDDPGDFVRIEVEEALKNSKTRV
                     IPVLVDNAVMPVAEELPASMAELAYRNAVKVRTDPDFPHDMQNLIRHLKYIKGGRRLA
                     RSLWLFLPLILLLIPGFFLFSSQNEKEPPTLTPTVTVHPSETPTTVVSTPTPLVEPVG
                     VGEIMVLVAQIEQIGAQQRDVTRFIMDDLVQRFETEQLVPNVRIREYGDIIKSNTQAQ
                     EVSEQTGAFLVIWGQYDDESTTVNVQLGSLASRPDLVIDRDTIESSVDVRLRLKDERQ
                     ETLAYPILSALGSLYYAENNFLGSTRLVMSIDQLDASRPELLGNTAAAHTQRAVQVFL
                     SDPETASSEITQAIELDAANPLLYVFRSLVYQGMGNFALGRQDSDTAVRLAPDGWIVP
                     YYMRGNESLITGDISSGIDAYTRVIEERPDDWYPYNMRGYLYFLAQNYEKARADNEKS
                     IELGPEAEWPYMWGTLVVLRQGRLNDVPTYIMGIMNNQSKNPVFVQRLMTTVFGEENG
                     KLLGYSMAAIGHLFFGQFDVSAQEADAVLAVMPTYAEMHMLKGLSYCNIDEYQKAEEA
                     YSAGLEVDPSFTMLHFLRAEVRGRLGDVNGAAEDLSVVAQSDISENLQPYIEAARSGQ
                     FSCKDMTSPR"
     gene            complement(5597..6301)
                     /locus_tag="VFQ23_17555"
     CDS             complement(5597..6301)
                     /locus_tag="VFQ23_17555"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9908460.1"
                     /translation="MANERTYPCLPCRDIDESVAFYESIGFKRTYRQVRPNPYAVVAL
                     EEIHIHLFGMDGFNPEDSYGTTIITVPDPDSLYHAFAAGLRKVHGKLLVSGIPRMTRP
                     RKKFGTVRGFSVVDPGGNWLRIYKLGETEENDSAEKAEGLAQIFYVATRLGDAKGDEA
                     LALKTLESGLTRFPDSPVIDLAKAYLYRAELAVRTQDHELAKSSLAAAQSLTLTDDEK
                     DGLADEFAHVTELLQQ"
     gene            6479..6808
                     /locus_tag="VFQ23_17560"
     CDS             6479..6808
                     /locus_tag="VFQ23_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908461.1"
                     /translation="MIEVLLGSKNAERVLIYIFSRAEGYAREIASFYGTDLKPIQMQL
                     DKFEQGGVLVSRAVGRTRPYEFNPRYPFLSELKALLEKTLSFYPAKEQEELRMNRRRP
                     RARGKEL"
     gene            6805..7089
                     /locus_tag="VFQ23_17565"
     CDS             6805..7089
                     /locus_tag="VFQ23_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908462.1"
                     /translation="MKRIKDTSQVELAAYIQDSLEADGILVVLSGGSAVSFYSSNKYI
                     SKDLDLINTGFVRRSKIKAVMEELGFKEKDAILSTPKQLSLSNFPMDRFR"
     gene            complement(7402..7890)
                     /locus_tag="VFQ23_17570"
     CDS             complement(7402..7890)
                     /locus_tag="VFQ23_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908463.1"
                     /translation="MRQLFVFVTVLVLLAACTPATEMFTAMPATEAPTSIPATSTPVS
                     CQPSEILESQVAFGEIQGDMQSDGTLWALLFFKAVYVDAEAKIVWRITGEGGEFKAQA
                     RSEGGTILQPVWEQYHESSTWERPGEEWGTGFNFPESGCWTITVTYGETIGMITLKVL
                     GS"
     gene            complement(8011..8400)
                     /locus_tag="VFQ23_17575"
     CDS             complement(8011..8400)
                     /locus_tag="VFQ23_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908464.1"
                     /translation="MPSKSKRSLQDVVGKQTYDVWVDMLRELVPDGRTHRLSVVIAGM
                     LMYAVDKSMQINHRNEEEHSVAMSLIDATDTSDPDSIKELIHDVVDRLFKDAGVKYER
                     VSKRGEHYSIADEIYNEFSSWYDYPWD"
     gene            complement(8677..>8908)
                     /locus_tag="VFQ23_17580"
     CDS             complement(8677..>8908)
                     /locus_tag="VFQ23_17580"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET9908465.1"
                     /translation="LQRQSWNVRPLCPSDISPFSKSENRESRREMTHAEKLLWQEIRA
                     NKLGVRFRRQQVTPALACDLLGKSCKCSGIHR"
BASE COUNT         2117 a         2291 c         2265 g         2235 t
ORIGIN      
        1 agacggcaag ttactttttg ccaaaggcta cggctacgct gatgttgaga atgccatccc
       61 ggtcgaccct gagcagacga tctttcgtat tggctcagtc accaagttgt tcacctggac
      121 ggcggtcatg cagctcgcgg agcagggcaa gctggatctg gacgcggata tcaacaccta
      181 tctcgacttt cgcatcccgg acacctatcc acaaccgatc accctcacgc acctgttgac
      241 ccatacgcca ggatttgaag accggttttt cgaggtcctg gcatcagatg tcgatgatct
      301 ggttcctgtg cgcgaatggc tggtctccca tatgcccgcg cgggtgcgtc cgcctggctt
      361 ggctgcggcg tactcgaact atggtacgtc cctggcgggg tacatcgtgg cgcaggtctc
      421 gggccagcct tacgaacaat acatccagga aaatatcctt aacccactgg gcatggtgaa
      481 ttcaactgca cagtcaccga taccgtcaga tctgctcgcc caggcctccg tcggatatac
      541 atatgaagac ggcgcttttc agtcattccc cgattacctg ggtcagccgg cgttcgtgcc
      601 ggccgcctca ctatcagcca gcgccaccga tatcgcccgt ttcatgatcg cgcatctaca
      661 ggatggccgc tacagcgatg caaatacccc tgtggcgcgc attctggaag aggcttcggc
      721 ccagcaaatg cacaccactc tgtacacgcc tgacccgcga ctcctgggca acgcttacgg
      781 cttttttgat ttcagtgaca acggtcagcg cacgctcggg catagcggtg aatcgcatcc
      841 gttcaatacg ctattgctgc ttctgcccga ccagaacctg ggcgtatttg tttcctacaa
      901 cagcgagggg ggtgggaacg tgacgaatca gcatcttggt ttccagagag cgttcttcga
      961 ccactactat ccggcacctg cagcagcttc aatacaacct ccggcaaatt tcacggagcg
     1021 ggccgcccgg ttcgagggca gctatcgact cactcggagt tcttatacga ctctcgaaaa
     1081 ggtttcaaca ctcatgggtg ggacagtttc aatcagcgat tccggtgatg gcgcgctgct
     1141 gttcacgaca ccctggggcg agtggcgatt tgtggaagtg gatccgctct acttccgcca
     1201 ggttgacggt caattcagcc tggtctttcg tgaagacgac cagggacgca ttacccatat
     1261 gtttgccaac gtcacgccca tgtttgcttt tgaaaagctg aactggtacg aaacccccgc
     1321 ttttaacatg ggacttctgc tggtcagcct ggtgatcttc ctgtccatga tcctggtggc
     1381 tttgattcgc tttattcgga accgccgccc gggcagtgac ccgaaccccg cgccgcgcgg
     1441 tgcccgcatg gctcagggga tcatcctggc gatcagcgtt ctgaacctgc tgttcgtggt
     1501 cggtacagcg ctgtggggca atccctttcc tgtgttcgga gtctctatga tctataagat
     1561 tgtgctgggg ctgggagttt tatccgccgc gctgacagtc ggcgcgctgg tctacaccgt
     1621 ggcagcttgg aagaacaact actggggcat tgctggccgt gcctattaca cgctggtgac
     1681 agttgcagca gttggttttg tctggtttct gaactactgg aatctgctgg gctggcggtt
     1741 ctagccggca agcgtttcat gctggcaggg aaggtattca agctctgcca gcatgaaaaa
     1801 aactgtgcag gcagattttt aaaataagga aattcgaaat gtcaatcaca ctggaaaaaa
     1861 aacaacctaa tacgacgcag ggcaacgacc cacttgccct gctacaggaa aataatcaat
     1921 ataacatttg gcagatcctc accatctggc tgaccgggag tgccctgatg tggatcatgg
     1981 gctgggtgat ctacccggcc ctgagccgag acttatctgt cgtagaggca ggcctgctac
     2041 gaattagact tctcaccgtg gggttgatct ggcaatttct gctctcgatg atcatcctgt
     2101 atatggaaaa aggtaatttg cgtctcacta caatccgccg ccggttctgg ctgaacactc
     2161 ccatctcacc caaaacgggg cagaaagaca ggcggctttg gtggttgatc ctgccgttgc
     2221 tcatgttgat ggtcgtgctt gaactgggac ttgacccgat tctcaacggc acgtggatca
     2281 agcttttccc tttcctggcg gaaccgcccg ggtatagccc agcgattttg ttcgctcccg
     2341 aattgcggtc tctctggctc ggggcatggg acctgctcgc gctccaggtg gtgctctccg
     2401 tgttcaatac atttttgggc gaggaattcc tcttccacgg tgtgttgttg cccaaaatga
     2461 acggtgtctt tggcaaatgg gactgggccg cgaatggaat tctcttcggg ctatatcatc
     2521 tccaccagcc gtggggactg ccgggaaata tcgtgtctgg cttgctgctg gctttcgtcg
     2581 cgaaacgtta tcgcagcaat tggtttccca tcctcttgca ttccgggcag agtgtttact
     2641 tcatcttttt gatcctcggg ctggtgctgg ggctggcata actacaaagc gacccgcttc
     2701 aagcgggtcg ctcttcttat ctgtaacaga aaacatctct cgcaggattt ttatcgagga
     2761 tacaaaatca tctgtgtgca tcggaacaag gcaatcgttt tcccgctggt ctcgtcagtc
     2821 accactgcgt cccaaacctg agtcgttcgt ccactgtgga ctcgggttgc cacgcagacg
     2881 atccctccct cacgcgttgt gccgagaaaa ttactcttta gctctatcgt tgtgaagttc
     2941 tgcgctccat ccggcaggtc cgtaaaagtg ccatagccgc agcttgtatc tgccagggcg
     3001 atgatcgtgg cggcgtgcag gtatccgttg ggggcgagca attcggggcg taacgtcagg
     3061 cggctggaga ggcgccctgg ttcatagccg gtgaattcga tgccgagtaa ccctggcagg
     3121 agtccctcgc cgcgggcatt gagttggtca agggtggtca tgaatcaaag tatagcatgt
     3181 caatttttat aaaagttggg agtgtccaaa agtatgggct aacagctgtt ttgccacaga
     3241 cactcgcacc tgcttcgggg caggtggaaa aaactcaaaa gactctgtgg gctcccctag
     3301 cgccgcccgt gatttgctcg agcaaatcag gcaggtgtgt ggcttcgccc acgaaattag
     3361 acactctcta aaagttgtac tatagaatag ttttatgttc aggagtacaa taaaaatcta
     3421 acatctaccg ctcgcgcagg aggaaacatg ccgcgtattt ttatctcata caggcgatca
     3481 gatagtggtg tattcacggg ccgcatccac gatcaattaa aagccaggtt tggcgcgaat
     3541 aatgtgtttc gtgatgtata caatatccct gccggcagtg acttccgaac ggtgttaaat
     3601 gaagaggtgg gcagcacgga tgtctgtctg gtgatgattg gccctcaatg ggtaacgaca
     3661 accgatgctc aggggaaacg ccgcctggat gaccccggcg attttgtgcg catcgaagtg
     3721 gaagaagcgc tcaagaactc aaagacgcgt gtgattcccg ttttggtcga taacgccgtt
     3781 atgccggtcg ccgaagaact gccagccagc atggcggaac tggcctatcg caacgcggtc
     3841 aaggtgcgga ccgatccgga cttcccgcac gatatgcaga acctgatccg tcacctgaag
     3901 tacataaaag gaggacgacg cctcgcgcgc agcctgtggc tgttcctccc cttgattttg
     3961 cttctcattc cgggcttctt cttattttct tcacaaaatg agaaagaacc tcctacgctt
     4021 actcctacgg tcacagttca tccatctgaa acgccaacga cggttgtgtc aactcctacc
     4081 cctttggtcg aacccgtggg agtcggtgaa attatggtgt tggtcgcgca gatcgagcag
     4141 atcggcgcgc aacaaagaga tgtgacgcgc tttatcatgg atgatctggt gcagcgcttt
     4201 gaaacagaac aactggttcc caatgtccgc attcgagagt atggagatat catcaaatcg
     4261 aatactcagg cgcaggaggt gtcggaacaa acgggggcgt tcctcgtgat ctggggacag
     4321 tacgatgatg agagcacaac ggtcaatgta caacttggat cgctggcatc tcgtccagac
     4381 ctggtaattg accgcgacac gattgagagc tcagtcgatg tacgcttaag attaaaagat
     4441 gaacgccagg aaaccttggc ctaccccatt ttgagcgcac tgggatccct gtattacgct
     4501 gaaaacaact ttctaggctc gacgcgtttg gttatgtcta tagatcaatt agatgcttcc
     4561 agacctgagc ttcttgggaa tacagctgct gcccatactc agagggcagt tcaggtattt
     4621 ctctccgacc cggaaacagc atcgagtgaa attacacagg cgattgaact ggatgcagcc
     4681 aacccgctcc tttatgtatt tcgtagtttg gtgtatcagg ggatgggaaa ttttgcgttg
     4741 ggcaggcagg attcagacac cgcggtacgg ttagcgccag acggctggat tgttccttac
     4801 tatatgcgag gaaacgaatc cctaattaca ggggatatat ccagcggtat cgatgcttat
     4861 acccgtgtca ttgaagaacg accggatgac tggtatccat ataacatgcg tggctatctg
     4921 tatttcttag cgcaaaacta tgagaaggcg cgagctgata acgaaaaatc cattgaactg
     4981 ggaccggaag cggaatggcc ttatatgtgg gggacgttgg tcgttctcag gcagggacgt
     5041 ctgaatgatg tccctacata cataatgggc atcatgaaca accagtctaa gaacccggtg
     5101 ttcgtgcagc gattaatgac caccgtgttt ggtgaagaga atggcaaact cctgggatac
     5161 tccatggctg ctattggtca ccttttcttc gggcagtttg acgtctctgc gcaggaggct
     5221 gatgctgtgc tggcagtaat gccaacgtat gcggaaatgc atatgctcaa ggggttgagt
     5281 tactgcaata tagacgagta tcaaaaagcg gaagaggcct actccgcggg gctggaagtc
     5341 gacccatcct tcacgatgct tcatttcctg cgcgccgaag tgcgcggcag gcttggagac
     5401 gtgaatggcg ccgcggagga tttgtctgtt gtggcgcaga gtgatatcag tgagaatttg
     5461 cagccatata ttgaagcagc acgatcaggg cagttctcat gcaaggacat gacatcaccc
     5521 agataaagac aacacaggtc ttgcgcttgg ccggcgttga acagcgaaat gttctccctc
     5581 tccaagtgct tttgagctac tgctgaagga gctcggtcac gtgagcgaat tcatctgcaa
     5641 gcccatcctt ttcatcgtca gtcagcgtaa gggattgcgc tgctgccaat gatgatttag
     5701 ctaactcatg atcctgggtc cggactgcca gttccgctcg gtataaatag gcctttgcga
     5761 gatcgattac tggggaatct ggaaaacgag tgagtccact ttcgagcgtc tttagcgcaa
     5821 gcgcttcatc acccttggca tctccaaggc gggttgcaac ataaaagatc tgtgccaacc
     5881 cttccgcttt ctcggctgag tcgttctctt ctgtttcacc aagcttataa attcgtagcc
     5941 agttcccgcc cgggtcaacg acgctgaagc cgcgcaccgt gccgaatttc ttgcgcggtc
     6001 gcgtcatgcg tgggatgcct gagaccagca gcttgccatg tacttttctc agtccggcag
     6061 caaaggcatg atataagcta tccgggtctg gtacagtgat gatggtagtg ccataggaat
     6121 cttcagggtt gaatccatcc atgccgaaga gatggatgtg tatctcttcc agcgccacga
     6181 ctgcatatgg gtttgggcga acctgccggt aggttctttt gaacccaatc gattcataga
     6241 atgcgaccga ctcgtcgatg tcgcggcagg gcaggcatgg ataggtacgt tcgtttgcca
     6301 tgagtgagct ccttcgtaaa ggtttctaag ggggataatt ataaatgctt ccccggttac
     6361 ttgatcgatt tttgaatcgc caacaaataa actaaaaccc gagctgctat tttcaattgc
     6421 gatataatga cgttaattta acgtcatgtg acgtcagtta aacgtcataa ggatttccat
     6481 gatcgaagtg cttttaggtt cgaaaaacgc tgaacgtgtg ttgatttaca tcttctcgcg
     6541 agctgaagga tatgcgcgtg aaatcgccag tttttatgga acagacttaa agcccattca
     6601 aatgcaattg gacaagtttg agcaaggcgg agtcctcgtt agccgcgccg tcgggcggac
     6661 gcgaccgtat gaattcaatc cacgttatcc attcttgagc gaactaaagg cgctgctcga
     6721 aaaaacgctt tcgttctatc ctgcgaaaga acaggaagaa ctccggatga accgccgccg
     6781 tccacgcgcc aggggcaaag agctatgaaa cgaataaaag acacgagcca ggtggaactg
     6841 gcggcgtata ttcaagactc tttagaagca gatgggattc ttgttgtgct ttcaggcggg
     6901 agcgcagttt cgttttatag cagcaacaag tacatttcaa aggacttgga tttgatcaac
     6961 acaggctttg tccgacgaag caagatcaaa gcagtgatgg aagaactggg tttcaaggaa
     7021 aaggacgcta ttttatccac cccgaaacaa ctttctttgt cgaatttccc gatggaccgc
     7081 tttcggtagg tgaagaacct gtaaaagaag tcagtgagtt cgaactttca acagggactt
     7141 tgcgagtgct ctctccaacg gactgtgtca aagacaggtt gtgtgcgttc tatttttgga
     7201 atgacttgca gggattggag caggcaattt tagtgacgaa gagtcaacaa gtgagtttga
     7261 aagaaataaa acgttggtca aaagttgagg ggaaagaaaa agaatataaa acttttacag
     7321 acaaacttgc tctttaatgt tcacgatcac acagatagtg aagcgtctcg cgaaacttca
     7381 tctcagaatt ccgagactcc attatgagcc cagcactttc aatgtgatca tgcctatggt
     7441 ttccccataa gtaactgtga tcgtccaaca gcctgattca gggaagttga atcccgttcc
     7501 ccattcttca cccggacgtt cccacgtaga actttcgtga tactgctccc agacaggttg
     7561 aaggatcgta ccaccttcac ttcgcgcttg cgccttgaat tcaccgccct ctccagtgat
     7621 gcgccagacg atctttgcct ccgcatcaac atataccgct ttaaaaaata acaacgccca
     7681 tagtgttcca tcagattgca tgtcaccctg aatctcccca aaagcgacct gagattccag
     7741 aatctccgaa ggctgacagc tcaccggcgt tgatgttgcg ggtatgcttg tcggtgcttc
     7801 tgttgcaggc atagcggtaa acatttccgt tgcgggcgtg catgccgcaa gcaaaaccag
     7861 aacagtaacg aaaacaaata attgacgcat cgttgattct tgaaaagatt atatctttgt
     7921 ttattatgcc aaaaccgttt ctttgatcat ccccaccact gcagataaat ttctcaccgc
     7981 ctcatcattc ctcaagccct gaaacggcaa ctaatcccac ggataatcgt accaactcga
     8041 aaactcgttg taaatttcat cagcaatact gtaatgttcg ccacgtttgc tgaccctttc
     8101 atacttaaca cccgcatctt tgaacaatct atctacaaca tcgtggatca attccttgat
     8161 ggaatcagga tcgcttgtat cagttgcatc aatcaatgac atggcaaccg aatgttcttc
     8221 ttcgttccta tggtttatct gcatggattt gtccacggca tacatcagca tccccgcaat
     8281 cacgactgat agacgatgcg tccgcccgtc agggacaagt tcgcgaagca tgtccaccca
     8341 cacatcataa gtttgttttc caacaacgtc ttgaaggctt cttttggact ttgacggcat
     8401 gacgtaactc cttcaaaaat tactttgcac caatccctta attttaccca ccaccgcgga
     8461 tcgcgaagca tccctcacga ccttatcatt cccaaagcgg actactttca gccccatctc
     8521 actcagcacc ttctcccgca cccccacccg tcctccccca aatccgacga tgtagacttt
     8581 gaatcttcat actaacttta gcttgtcgta tttgggggta ctctgcgagt attctccgcg
     8641 aaggtgcccg gaggacggag ggggcgacag taaaagtcaa cgatgaatcc ctgaacactt
     8701 gcatgatttg ccaagcaaat cacacgcaag tgcaggtgtg acctgctgac gtctaaaccg
     8761 aacacccaac ttgtttgcac ggatttcctg ccacagcagt ttttcagcat gagtcatctc
     8821 acgccgactc tccctatttt cgctctttga aaatggggag atgtccgaag gacagagggg
     8881 tcgcacgttc cagctttgcc tttggaga
//
LOCUS       DASTJP010000596         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_574967, whole genome shotgun sequence.
ACCESSION   DASTJP010000596 DASTJP010000000
VERSION     DASTJP010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574967"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1063)
                     /locus_tag="VFQ23_17585"
     CDS             complement(107..1063)
                     /locus_tag="VFQ23_17585"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET9908466.1"
                     /translation="MNDGTTGVIKERLEKSKNVMIASHIRPDGDAIGSLLGLGLALLD
                     AGKSVRMVLVDGVPASFKHLGGSERIVKEPHEEYDTFVTVDSADFKRLGKLFENFGRP
                     DINIDHHKTNENFGKINLIEAEEVATSAILARHLPEWGYNITRPIAAALLTGIITDTL
                     GFRTSNTNPTALRLCAMLMETGVDMTELYMKSLVMKTFPAARYWGAGLSSLEQKNGMV
                     WATLTLNDRKLAGYSGNDDADLINMISAIDGNKVGMIFVEQSDNHVKISWRALEPGID
                     VSQVAKHFNGGGHAAAAGADIPGGLNEIQPLVLKTTQEMLSI"
     gene            complement(1117..1467)
                     /gene="rbfA"
                     /locus_tag="VFQ23_17590"
     CDS             complement(1117..1467)
                     /gene="rbfA"
                     /locus_tag="VFQ23_17590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9908467.1"
                     /translation="MPSGIRLQRIADRIRQELSEVLIREINDPRLKQIYVTDVKVDRE
                     LAYADVYVSAVEGVSRSADVLAGLESASGFLRRTLASRVELRAFPRLRFHWDMTPENA
                     DHIEQILAELRKKK"
     gene            complement(1542..>2430)
                     /locus_tag="VFQ23_17595"
     CDS             complement(1542..>2430)
                     /locus_tag="VFQ23_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HET9908468.1"
                     /translation="TLESGDTVVAGSAHGRLRALSDYKGKPVKKAGPSTPVAVMGLND
                     VPSAGDIFEVVDSEKEARLIVAERLALAKSQAQTKKKVSLEDLFSSVQAGEAKELNLI
                     VKADVQGSLDPIVSELNKLGEGEIGIKVLYSETGNIGDNDVMLASASNAIIIGFNVQV
                     DVPARRMAEKEGVDIRLYEIIYRMTEDIEKALSGMLEPETKEQIIGRAQVLAVFTASK
                     FGKVAGCRVTEGELRRNAKVRLYRGTDIVYEGDMGSLRHEKEDVREVRQGFECGVGFK
                     SFNDIQVGDQLVCYTIEKS"
BASE COUNT          604 a          656 c          525 g          645 t
ORIGIN      
        1 ccccggagat tgcattcttt acatcttgac tgttcacttt cattcctcct tataaggcaa
       61 tgatcattat aattatgaca tacttcgcta aattagtgat gagaatttat atagacaaca
      121 tttcctgtgt tgtttttaat accaatggct ggatttcatt cagcccacca gggatatcgg
      181 cacccgcggc ggcagcatga ccgccgccat tgaagtgctt cgccacttgc gacacatcaa
      241 tacccggttc gagggcgcgc caagagattt tgacatgatt gtcgctttgt tcgacgaaaa
      301 tcataccgac tttgttccca tcaattgcag aaatcatgtt gataagatcc gcatcatcat
      361 taccgctata acctgcaagc ttacgatcgt taagcgtgag tgtagcccac accattccat
      421 tcttctgttc caggctggaa agcccggctc cccagtaacg tgcggcaggg aacgttttca
      481 taactaacga tttcatataa agctcagtca tatccacacc ggtttccatc aacatggcac
      541 acagacgcaa agcagtggga ttggtgttcg aggtgcggaa accgagcgta tctgtaatga
      601 tgcctgtcaa cagagctgca gctattggcc tggtaatatt gtatccccat tccggtaagt
      661 ggcgggcaag gattgcggaa gttgcaactt cttccgcctc aattaagtta attttcccga
      721 aattctcatt ggtcttgtga tgatcaatgt tgatatcggg ccgaccgaag ttttcgaaaa
      781 gttttcccag ccgtttgaaa tcagcagagt ctacagtgac aaatgtatcg tattcttcgt
      841 ggggttcctt aacaattcgt tcgcttcccc ccaaatgctt aaaggatgct ggcacgccat
      901 ccactaaaac catccgaact gattttccag catcgagtaa agctaaaccc aagccaagca
      961 aagaacctat tgcatcccca tctggtcgga tatgtgacgc aatcatgacg ttctttgatt
     1021 tctccaggcg ttccttgatc accccggttg taccatcatt cataaaatgt gagttcctct
     1081 tatatgtact aatttgtttc cagcaagaca cgaagtttat ttcttttttc gtaattctgc
     1141 aagtatttgc tcgatatggt cagcattctc gggcgtcatg tcccagtgga agcgcaggcg
     1201 cggaaaggcg cgcagctcca ctcgcgaggc cagagtccgc cggagaaaac cggaggcgga
     1261 ctccaaaccc gcgagcacgt ctgcagaacg tgaaacgcct tccactgctg aaacatagac
     1321 atcggcgtac gcgagttccc tatctacttt tacgtccgtc acgtatattt gtttcaaacg
     1381 tggatcattg atctcacgga tcaaaacctc cgagagttcc tggcggatcc ggtccgcaat
     1441 acgctgaaga cgaattcctg atggcatgtt cttttcatca ccttgcgaag gctgatctcg
     1501 aagaggagac ctccacgatt aaaccttcgc aagatccgta attacgattt ctcaatagta
     1561 taacaaacca gttgatcgcc gacctgaata tcattgaaac tcttaaagcc gacgccgcac
     1621 tcaaatccct gccggacctc tctcacatcc tctttttcat ggcgcaggga acccatgtca
     1681 ccttcataaa caatatcagt gccgcgatac agacggactt tcgcattgcg gcgcaactca
     1741 ccctcagtta cacgacagcc tgccactttg ccaaacttgg atgcagtgaa gaccgcaagt
     1801 acctgggcgc gtcctatgat ctgttccttg gtctcaggct caagcatacc actcagggct
     1861 ttttcgatat cctcggtcat gcgataaatg atctcgtaaa ggcggatatc tacgccttcc
     1921 ttttccgcca tgcgccgggc tggaacatcc acctggacgt taaagccgat aataatggca
     1981 tttgaggcgg aagcaagcat gacatcgttg tcaccaatat tacctgtttc agaatacaga
     2041 actttgatgc cgatctcacc ctctccgagc ttgttcaact cgctaacgat cgggtcgagc
     2101 gaaccctgta catccgcctt gacgatcagg ttcagttcct ttgcttcgcc cgcctggaca
     2161 ctgctaaaca ggtcttcgag cgataccttc ttctttgttt gcgcctggga ttttgccaat
     2221 gccagacgct cggcaacaat caggcgcgct tctttttcag aatccaccac ctcaaaaata
     2281 tcacctgcgg aagggacatc gttcaatccc atcactgcta caggggtgga gggaccggcc
     2341 ttttttacag gcttaccttt gtaatctgaa agggcgcgca atctgccgtg agcactcccg
     2401 gctacaacag tgtcgccgga ctcaagagtt
//
LOCUS       DASTJP010000597         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1208782, whole genome shotgun sequence.
ACCESSION   DASTJP010000597 DASTJP010000000
VERSION     DASTJP010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208782"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFQ23_17600"
     CDS             <1..547
                     /locus_tag="VFQ23_17600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908469.1"
                     /translation="TLETHVDSVPHIVASSEPETEQVCVRSCSASDVDPLRAMARSNH
                     HNTRFSADRHFSAEQCSALYERWIEASCKGSADSVFVAESRIGLAGYITCHLEQQFDG
                     KIGLLGVEETARNLGIGRKLVSEALGWFAAHGARRIQVVTQGQNIAAQRVYLRSGFLT
                     KSVQLWYHRWFISNGRNSTDE"
     gene            540..1682
                     /gene="rffA"
                     /locus_tag="VFQ23_17605"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             540..1682
                     /gene="rffA"
                     /locus_tag="VFQ23_17605"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HET9908470.1"
                     /translation="MNEYDIPFNRPYVASAVGSYIAQAIATGHISGDGLFTAKCGAFL
                     ERELRSIRALLTTSCTHALEMAALLLDIQPGDEVIVPSFTFVSTINAFVLRGAQPIFI
                     DIRPDTLNIDETLLELAITPRSKAIVPVHYAGVGCEMDSLLAIAAHHGLAVVEDNAHG
                     LFGKYKGQYLGTFGHLATQSFHETKNFSCGEGGALLINDSRFVERAEILREKGTNRSR
                     FFRGQIDKYTWVDLGSSYLPSDILAAFLYAQLEQWTQIQAKRRRTWEFYLGHLAGWAH
                     DHGVGLPVIPAHCEQAYHMFYLLLPSLERRQALITHLKQQGILSVFHYQPLHISDMGQ
                     RYGGRPGDCPVTEDISDRLLRLPFYNDLTEEQQCRVVAAIHDFDCG"
     gene            1675..2511
                     /locus_tag="VFQ23_17610"
     CDS             1675..2511
                     /locus_tag="VFQ23_17610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9908471.1"
                     /translation="MDNCHLQGKSIVDFTASYEPNFFKTLFEVEDHHFWFRARNMLIG
                     RLFEQLSNNFSQGYRVLEIGCGTGNVLRVLEQACSRGTVFGMDYFHEGLCYARKRTEV
                     LLLQGDMRRPPFQTKFDLIGLFDVLEHLPDDRQVLTDIHSMLSDRGLLVLTVPAYSSL
                     WSYFDEASGHYRRYEPEDLLLKLTNAGFVAEHFTPFMMSLFPILVLYRRMAGLIQYKQ
                     QANRSTQELTTNELRVWPIFNDVFYKLLSIETFFLSRRFKLPIGTSLLVVASKSGTNK
                     IA"
     gene            2527..>2957
                     /locus_tag="VFQ23_17615"
     CDS             2527..>2957
                     /locus_tag="VFQ23_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908472.1"
                     /translation="MKNIKHNINDHIQPAPAYANAGQGALSNIRNQDYRTDDNERETY
                     VVPSWRDCFNVVWSLYLPIRLLLSLWIAILAAFGPFRTTAHDIGGWLWKLLLEPWYEW
                     DVLRYVDIARDGYGINDGRAAYHPLLPFLMRGIGDILGGQY"
BASE COUNT          709 a          686 c          753 g          809 t
ORIGIN      
        1 gacacttgag acacacgtag atagtgttcc acatattgtc gcatcctcgg agcctgaaac
       61 cgagcaggta tgtgttcgat catgctctgc ttcggatgta gacccgctgc gggcgatggc
      121 gcgttccaat caccacaata cacgtttctc cgccgacaga catttttcgg ctgaacaatg
      181 ctcggcattg tacgaacggt ggatcgaagc atcgtgcaaa ggaagcgccg attctgtgtt
      241 tgtcgccgag tctcgtattg gcttggccgg ctatatcacg tgtcaccttg agcaacagtt
      301 cgatgggaag attggcctgc ttggggttga agaaaccgcg cgtaatctcg gcatcggccg
      361 aaagttggtg tcagaggcgc ttgggtggtt cgccgcacat ggcgcccgac gtattcaggt
      421 tgttacgcaa gggcaaaaca tcgctgctca acgtgtgtat ttgcgcagtg gatttctgac
      481 gaaatctgtg caactctggt atcatcggtg gtttatttct aatggaagga actcaaccga
      541 tgaatgagta tgatatacct ttcaatcgcc cgtatgttgc cagcgccgtg ggatcatata
      601 ttgcccaggc aattgccacc gggcatattt cgggcgatgg actctttacc gcaaaatgtg
      661 gcgcatttct agagcgcgaa ctgcgcagta ttagagcatt actcaccacc tcctgtaccc
      721 atgcgttaga gatggcggcg ctgctgctcg atattcagcc gggtgatgaa gtcatcgtgc
      781 cttcttttac ctttgtttca accatcaatg catttgtgct ccgcggcgct caaccaatct
      841 ttatcgacat acggcccgac acgctcaaca tcgatgaaac gctgcttgaa ctggcaatta
      901 cacctcgctc aaaggcaatc gttccagtgc attatgcggg cgtgggttgc gagatggatt
      961 cattgttggc tatcgctgct caccacggat tagcagttgt cgaagataac gcccatggtc
     1021 tgtttggtaa gtacaagggc caataccttg gcacattcgg ccatttggcg acacaaagtt
     1081 ttcatgaaac aaaaaatttc tcctgtggcg agggtggcgc gttgttgatc aacgactcgc
     1141 gttttgtcga gcgcgccgag atactgcgcg agaaaggtac taaccgcagc cgcttttttc
     1201 ggggtcagat cgataaatat acctgggtcg atctcggatc aagttacttg ccgtcggata
     1261 ttttggcagc gttcttgtat gcacaactcg agcaatggac acagatccag gcgaaacggc
     1321 ggcgcacctg ggaattttat ttgggacatt tagcgggttg ggcacatgat catggggtcg
     1381 gtttgccggt gataccagcg cactgcgagc aagcatacca catgttctat ttgctgttgc
     1441 cctcgcttga gcgacgccaa gcgctgatta cacatttaaa gcagcaaggc attctgagcg
     1501 tatttcatta ccagccgttg cacatatcag acatgggcca gcgttatggt ggccgacccg
     1561 gtgattgccc ggtgacggaa gatatcagcg atcgattgtt acgcttacct ttttacaacg
     1621 atctgactga ggagcagcag tgccgcgttg tcgcggcgat tcacgatttt gattgtggat
     1681 aattgccatt tgcagggaaa gagcatcgtg gattttaccg ctagctatga accgaatttc
     1741 tttaagacat tgtttgaggt cgaagaccat catttttggt ttcgcgcaag aaacatgctg
     1801 attggtcgcc tattcgagca attgtcaaac aattttagtc aaggctatcg cgttctggaa
     1861 ataggatgtg gtacaggcaa tgtactacgc gttctcgaac aagcctgttc cagaggcacc
     1921 gtttttggaa tggattattt tcacgaaggg ctatgttacg cgcgaaaacg cactgaagtt
     1981 ttgctgctcc aaggtgacat gcgccggccg ccatttcaaa cgaaatttga tctgattggt
     2041 cttttcgatg tccttgaaca tctaccggat gaccggcagg tattgactga tattcattcg
     2101 atgttaagcg atcggggttt gctagttttg acggtgcctg catattcgtc actgtggagt
     2161 tacttcgatg aagcctctgg gcactatcga cgctatgagc cggaggatct tcttctaaaa
     2221 ctaacgaatg caggctttgt tgctgaacac tttactccgt ttatgatgtc actctttcca
     2281 atcctcgtat tgtatcgccg aatggcgggc ctgatacagt ataaacaaca agctaatcga
     2341 tccacccagg agctaactac gaatgagttg cgtgtttggc cgatattcaa cgatgttttt
     2401 tataaattat tgagtataga gacgttcttt ctttcacgcc ggttcaagtt gcctattgga
     2461 acatcattac ttgtcgttgc atctaaatca ggcacgaata aaatagcttg atgtgttagc
     2521 acaactatga aaaatatcaa acacaatata aacgatcata ttcaacctgc tccagcctac
     2581 gcaaacgcag gccagggagc actatcgaat ataagaaatc aagattacag aaccgatgat
     2641 aacgaacgag aaacttacgt ggtgccaagt tggcgcgatt gtttcaacgt agtttggtcg
     2701 ttatatctac cgatacgtct gctattaagc ctctggattg caatactggc tgcattcggc
     2761 ccgtttagaa cgacagcaca tgacatcggc ggttggcttt ggaagctctt gcttgagccg
     2821 tggtatgaat gggatgtcct acgctatgta gatattgccc gcgacggata cggcatcaac
     2881 gatggaagag cggcatatca tccgttgctc ccttttctca tgcgcggcat tggtgatatc
     2941 ttgggtgggc agtattt
//
LOCUS       DASTJP010000598         4550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1254862, whole genome shotgun sequence.
ACCESSION   DASTJP010000598 DASTJP010000000
VERSION     DASTJP010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254862"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFQ23_17620"
     CDS             <1..342
                     /locus_tag="VFQ23_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908473.1"
                     /translation="PDPIAATDLAADLGVRIYTVGIGSAAGATITVEGFTVHSQLNEP
                     ILQYIAETTGGAYYNAGSTEDLRRIYSDLEPKLSIKREEMEITSILAGISFLVILIGG
                     AVSLLWFGHIP"
     gene            380..1444
                     /locus_tag="VFQ23_17625"
     CDS             380..1444
                     /locus_tag="VFQ23_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9908474.1"
                     /translation="MSLLWPGSLFLFLLIPLIVVIYLLILRRRRGFAIRYSSLSLVRE
                     AAAHQSWLRRHLPFILVLLALSSLVMALSRPVATVTVPSNRATILLTIDVSRSMCSTD
                     IPPNRMEAAKDAAISFIQEQGAAGAQIGIVAFAGFAELVAPPTNNISELETAVQGLTT
                     ARRTAIGSAILRAIDAISEIDSRVAPSELGVAPDSVPPEGEYVPHIIVLLTDGASNAG
                     PFPLTAAQQAADRRIRVYTIGFGTINNTSPMNCGDFQEFDQFGGGGFGFGSPFAGGGG
                     GGFRRELDEATLKQIADMTGGSYYAATSASELNNVFRNLPTYLVTTRETIEISAFFTT
                     IAALLLIAAMVLSFLWHPVP"
     gene            1594..2829
                     /locus_tag="VFQ23_17630"
     CDS             1594..2829
                     /locus_tag="VFQ23_17630"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9908475.1"
                     /translation="MAEHRVTVYGAYWCPDCRRAKKFLGEQFVPYKWVDIEQDKNGEN
                     YVLQKNDGKRIIPTIVFEDDTFLVEPSNAELAKKLGLKTEAKKVYYDLIIIGGGPAGL
                     TAAIYASREGADVLLIERSGLGGQAGITVGLDNFPGFPEGISGQEFSERVTQQARRFG
                     VEILQAVDVERMEMEEGYHEVYTSDGKHYHSKAVLIATGASYKRLEVPGEDDYIGAGI
                     HFCATCDGPFYKGAKEIVVIGGGNSAVEEGLHLTNFAEKVTLLVRGDRLTASQIAIEK
                     VNEPTSRVEVKYNTVVDAIEGQDSKLKTIKYHNKDGNKTEEIHPAAAFIFIGQTPNTA
                     FLKDYIKLDKYGFALTGHDIGHDRQTMGIEPLPFEASVSGIFVAGDARHGSTKQVASA
                     VGEGAAASISIREFLRRSQ"
     gene            complement(2941..3339)
                     /locus_tag="VFQ23_17635"
     CDS             complement(2941..3339)
                     /locus_tag="VFQ23_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908476.1"
                     /translation="MTHVNKELEIHWKNIAPILCIRNEREYDMAVKRLNELLDEIGND
                     EKHPLYSLLDTLGTLIHAYEEEHYPIPEASGVDVLRFLMEEHALSQSDLPEVGSQGVV
                     SEVLNHKRELNVRQVRALAKRFKVSSAVFV"
     gene            complement(3323..3622)
                     /locus_tag="VFQ23_17640"
     CDS             complement(3323..3622)
                     /locus_tag="VFQ23_17640"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HET9908477.1"
                     /translation="MHIITRKTLVRFWEKYPDSQAALLRWFKIVQKTEFNSFAELRKV
                     FPSADKLDHWIIFNIGGNKYRLIASIHFNRGKVYIRHVLNHAEYDRGEWKNDTRK"
     gene            3745..>4550
                     /locus_tag="VFQ23_17645"
     CDS             3745..>4550
                     /locus_tag="VFQ23_17645"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9908478.1"
                     /translation="MSSNNRTVTVVGAGLAGLSAAYDLHRAGWKVTVLEGRARVGGRV
                     YSVRSFSNGLVAEGGGEFIEDRHVRMLAYAKQFNLQLGQVGSWQNQDSDWGALAGKTG
                     RLADESVWGTDLQPEIERMWNAVAGLAQYVPDPKQPQLSRDAQRFDAQSALDWCNTID
                     VHPIARSYFLQHIRSEYTAEPERFSLLDLARNAAMYYSTAERSSNWRVIGGNDLIPRA
                     LADALPDVRMNAVVTSIRILPDEVVVTYKQGDSYQTIQSDYAILAVPLTTA"
BASE COUNT         1111 a         1086 c         1207 g         1146 t
ORIGIN      
        1 cctgacccga ttgccgcaac ggacctggca gctgacctgg gcgtgcgtat ctatacggtc
       61 gggatcggca gcgcggctgg agctaccatc acggtagagg gctttaccgt ccactcgcaa
      121 cttaatgagc ctatacttca atatattgca gaaacgaccg gtggagcgta ttacaatgca
      181 ggcagtacag aagacctgcg ccggatttat agtgaccttg aaccgaagtt atcgatcaaa
      241 agagaagaaa tggaaatcac gtccattttg gcagggatca gttttcttgt cattttgatc
      301 ggcggtgcgg tatcgcttct ctggtttggc catatcccat gaatgtagct atggcttgtg
      361 tgcgtgtaag gagtaatcta tgagcctgtt atggcctggt tccctcttcc ttttcttatt
      421 aatcccgctt atcgtcgtaa tttatctttt gatcctgcgg cggcggcggg gatttgcgat
      481 tcgttactca agcctatctc tggttcgaga agcagcagca caccagtcct ggctgcgtag
      541 acacttaccg ttcatcctgg tgctccttgc gctttccagc ctggtgatgg ccctgagccg
      601 gccggtggca accgtcacgg tgccatcgaa tcgagccaca atactcctga ccatcgatgt
      661 atccagaagt atgtgctcga cggatatccc tccgaaccga atggaagccg ccaaggatgc
      721 ggccatctcc ttcattcaag aacagggagc cgccggagca caaattggta ttgtagcgtt
      781 tgccggcttt gccgaacttg ttgcaccgcc aacgaataat attagcgaac tcgagactgc
      841 cgtccagggg ctcaccacag cacgcaggac cgccatcgga agtgcgatcc tgcgcgcgat
      901 tgatgcgatc tctgaaatcg acagccgcgt cgcgccaagt gaattgggag tggctcccga
      961 ttccgtgcca ccggaagggg agtatgtgcc acatatcatt gtgctgctta ccgatggagc
     1021 cagcaatgcg ggaccctttc cgttaacggc tgcacaacaa gccgcagacc gccgcatcag
     1081 agtgtatacc attggcttcg ggacgattaa caatacatca ccgatgaact gtggcgattt
     1141 tcaggagttt gatcaatttg gtggaggtgg atttggtttt ggttcaccgt ttgcaggagg
     1201 cggcggcgga gggttccgcc gagaactgga tgaagcgacg ttgaagcaga tcgcagatat
     1261 gaccggcgga agctattatg ctgctaccag cgccagcgag cttaacaatg tatttcggaa
     1321 ccttccaacc tatctggtga ccacgcgcga gacgattgaa atcagcgcgt tcttcacgac
     1381 catcgcggca cttctcttga tcgccgccat ggtgctctcg ttcctctggc accccgtgcc
     1441 ttgaaatcga tagtgaccgt cacttctgaa gtgacggtca cttttataat acagttttac
     1501 aaaatcttac ataaaaatta acacaatcga atggggaatt aaatttgcct gccaggtcgt
     1561 caatagcaga gaaatcaaat aggagaatgt ctaatggctg aacatcgagt cactgtgtac
     1621 ggcgcctatt ggtgcccgga ttgtcgccgt gccaagaaat tcctgggaga acagtttgtt
     1681 ccatataaat gggtggatat tgagcaggac aaaaatggcg aaaattacgt cctgcaaaag
     1741 aatgatggca agcgcattat tccaacgatt gtgtttgaag atgacacctt tctggttgag
     1801 ccttccaacg cggaactggc taagaagctg ggtttaaaaa cagaagccaa gaaggtgtac
     1861 tatgatctga tcattatcgg cggtggaccc gccggtttga cggctgccat ctatgcctcg
     1921 cgtgaaggtg cggatgtgct gctgatcgaa cgctccggtc ttggcgggca ggctggcatc
     1981 accgtcgggc tcgacaactt tccgggcttc cccgaaggaa tcagtggaca ggaattctct
     2041 gaacgtgtga cccagcaggc gcgccgcttt ggcgtggaaa tcctgcaggc ggtagacgtc
     2101 gagcggatgg aaatggagga agggtatcac gaggtctata cctcagatgg caaacactat
     2161 cattcaaaag ctgttttgat cgcgacgggt gcatcctaca aacgtcttga agtaccgggc
     2221 gaagatgact atatcggtgc cgggattcat ttctgcgcga cctgtgacgg tcctttttac
     2281 aaaggcgcca aggagattgt cgtcattggt ggtggcaact ctgcggtgga ggaaggtttg
     2341 catctgacga acttcgccga gaaagtgact ttgctggtcc gcggggatcg cctgaccgcc
     2401 agccagatcg ccattgaaaa agtcaacgag ccaacttcac gcgtcgaagt aaagtacaac
     2461 accgtcgttg atgcaatcga aggccaggac tcaaagttaa agaccatcaa gtatcacaac
     2521 aaggatggca ataaaaccga ggaaattcat cctgctgccg cgttcatctt catcggtcaa
     2581 acaccgaata ccgccttttt gaaggactat atcaaactgg ataaatatgg ttttgcgctg
     2641 acaggtcatg atattggcca tgacagacaa acgatgggca ttgagcctct tccttttgaa
     2701 gcaagtgttt cagggatatt tgttgccggt gacgcgcgac atgggagtac caagcaggtt
     2761 gcctccgccg tgggagaggg cgcggcggcc tccatttcga tccgagagtt tttgaggcgg
     2821 agtcagtagt ttaagagttg acagtcactt ttgcgtagca ctcccgaaac ggagtgaagg
     2881 gagcaaagtg actgtcaact caataacatt tctaatgctt tcgacgactt gacgctcaat
     2941 tcatacaaac accgcgcttg aaaccttgaa tcgctttgcg agcgctcgta cctgacggac
     3001 attcaattca cgcttgtggt ttaatacttc cgatactact ccttgtgagc cgacttcagg
     3061 taggtcagat tgagatagcg catgctcctc catcagaaac cgcaatacat ctacaccgct
     3121 agcttcaggg attggataat gttcctcttc gtacgcgtga ataagcgtgc caagtgtatc
     3181 cagtaggcta taaagagggt gtttttcatc attaccgatt tcatccagta attcattgag
     3241 tcgttttaca gccatatcat attcgcgttc attacgaata cagaggatgg gggcaatgtt
     3301 cttccaatgg atttctagtt ctttatttac gtgtgtcatt tttccattct cctcgatcat
     3361 actctgcatg gttcagaaca tgacggatat atactttgcc gcgattaaaa tgaatagatg
     3421 cgatgagtcg atatttattg ccgccgatat tgaaaataat ccagtgatct agtttgtcgg
     3481 cggatgggaa aacctttcgt aactcagcaa agctattgaa ttctgttttc tgaacgattt
     3541 tgaaccaacg cagtaacgct gcctggctat ccgggtattt ttcccaaaac cgaaccagcg
     3601 ttttacgtgt gatgatatgc acgaaaagag tatatctcaa attgagatat aatgcaaggt
     3661 ggatttttag cttttataca agcattcata tatgttatag tttggacaaa acaacctcat
     3721 ctgggagaac ttcaatttaa ttttatgagt tcaaataatc gaacggtcac tgtggttggc
     3781 gcggggctgg cgggactctc cgccgcatac gacttgcatc gcgcgggctg gaaggtcact
     3841 gtgctggaag ggcgtgcgcg cgtgggtgga cgggtgtaca gtgtgcgaag tttttcgaac
     3901 ggtctggtgg cagaaggcgg cggggaattt atcgaagatc gccatgtgcg tatgcttgct
     3961 tacgccaagc agttcaattt gcagttgggg caggtgggca gctggcagaa ccaggacagt
     4021 gattggggag cacttgctgg aaagaccggt cgattggcag atgaaagtgt ttggggaaca
     4081 gaccttcagc ccgagatcga gcggatgtgg aatgcggtgg ctgggttggc acaatacgtc
     4141 ccggatccca aacagcctca actctcgcgt gacgcgcagc gcttcgatgc tcaatctgca
     4201 ttggactggt gcaacacgat agatgttcac cccattgcga ggagttattt cctccaacat
     4261 atccgttcgg aatatactgc tgagccagaa agattttcct tgcttgatct cgcacgaaat
     4321 gcagcgatgt attacagcac tgcagaacgc tcgtccaact ggcgtgtgat cggtggaaat
     4381 gacttaatcc ctcgggcgct tgcggacgca ttgcccgatg tccgcatgaa cgcggtcgtg
     4441 acctccatcc gcatcctgcc ggacgaggta gttgtcacct ataaacaggg cgattcgtat
     4501 cagaccattc aatctgatta tgcaattctg gcagttccgt tgacgaccgc
//
LOCUS       DASTJP010000599         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_474543, whole genome shotgun sequence.
ACCESSION   DASTJP010000599 DASTJP010000000
VERSION     DASTJP010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474543"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFQ23_17650"
     CDS             <1..801
                     /locus_tag="VFQ23_17650"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9908479.1"
                     /translation="SPVSRYRLCIDQARLKTAQGNLEGALALLDEAERLYIRSPLPDF
                     CPISAMKARIWVVKGKLTKALEWVCERGLSVNNAPSYLREFEHVTLARVLIARYKSDR
                     EPGATHGAIGLLERLLQAAEEGGRMGSMIEILALLALAHAAQGDIPLALVSLERALTL
                     AEPKGYVRIFLDEGKPMAELLKRAGKNKSSFLEKQAISPQPLVEPLSENELEVLRLLR
                     TELSGPEIARKRMVSLSTIRTHTQHIYAKLGVNNRRAAVRCAEELGLL"
     gene            1011..1283
                     /locus_tag="VFQ23_17655"
     CDS             1011..1283
                     /locus_tag="VFQ23_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908480.1"
                     /translation="MSETHALPEYYDGSELYEIRLKGHLDDRWADRFEGLTITLEENG
                     DTLLAGPVIDQAALHGLLKKVRDLGMLLVSVNFVNPGRAKTQEVKS"
     gene            1429..2148
                     /locus_tag="VFQ23_17660"
     CDS             1429..2148
                     /locus_tag="VFQ23_17660"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9908481.1"
                     /translation="MKTYRMNAVMAGVLYFLGTVFGVLSAVIGGEVLSSLNIGKPLVG
                     MDMLGLVVANSSRITVGAFFIFMMAISLVAMTVFLYPIFRKDSEELALGMVLFRGALE
                     GTGYIVSTVGFLALVVLGNEYIATGADSAALQSMGNLVYQFQVRLAPIGPIFFLIGAM
                     CLYLSFYRTRLIPRWLTVWGMIAVVFYMASTLLKFFHMGTGIGFYLEMIMAPQEMVMA
                     VWLIVKGFNPSAIAILFAKTE"
     gene            2241..2909
                     /locus_tag="VFQ23_17665"
     CDS             2241..2909
                     /locus_tag="VFQ23_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908482.1"
                     /translation="MQNYLLYACEILLVVVTPLLVLYIKGNWPLRKVIPPLVVIPILW
                     YFTYAPLHELSHAAGLYLVGGKVIDYKLIPRFWLGEFAGAWITPSGITQSWQQLTMSG
                     FPYMLDIVFFVVALFVFRRAFSRGPFFIGFAFMLLCLRPACDFVFEPIAFLSGDRNDF
                     YAIQQIVGPSVIWSFILIAIGLAVYSIVSTLSHLVSFSKQSIGQNTNETKHNIIPKER
                     GDLK"
     gene            2906..>3602
                     /locus_tag="VFQ23_17670"
     CDS             2906..>3602
                     /locus_tag="VFQ23_17670"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9908483.1"
                     /translation="MNTYRKTAITVGALFIVCTVASILGPTLAISANSPDYLDQLAGN
                     PDQIITAALLEFIWAAAGASIAIGLYPLLKKYNAALALGSVGFRVVENVFVLIGTLSL
                     LSLLTLSQEFIAAGAPEASSFQTLGTLLLAVRDWQLHVISGLAFSLGVLMYYAILYKS
                     NLIPRWLSGWGVLGAILALAATVLASFTRDFGLESAHTYLHIPIGLQELVFAVWLIAK
                     GFNPSAIAALSAKT"
BASE COUNT          836 a          888 c          893 g          985 t
ORIGIN      
        1 tcacctgttt cgcgataccg tttgtgtatt gaccaggctc ggctcaagac agcacagggc
       61 aacctggaag gggcactcgc gcttttggat gaagcggaac gcctatacat cagatcaccc
      121 ttgcctgact tctgtcccat atctgccatg aaagcccgga tatgggttgt gaaaggcaaa
      181 ttgaccaaag ctctggaatg ggtatgtgag cggggccttt ccgttaacaa tgcgcccagc
      241 tacctgcgtg agttcgagca cgtgacgctg gctcgagtgc tcatcgcccg gtataaaagc
      301 gaccgggaac ctggtgccac tcacggggca atcggcctac tggagcgcct cttgcaggca
      361 gcggaagaag gtggacggat gggcagcatg atcgagattt tggcgttgtt ggcgcttgct
      421 catgcggcac aaggcgacat tcccctggct ctcgtgtctc tggagcgtgc tctgacgctg
      481 gctgaaccga agggctatgt ccgcattttc ttggatgaag gcaaaccaat ggcggagttg
      541 ttgaaaagag caggaaaaaa caagtcttca ttccttgaga agcaagccat cagcccacaa
      601 cccttggtcg agccgcttag cgaaaatgag ctggaagtgc tcagactgct cagaaccgaa
      661 ttgagcggcc cggaaattgc ccgtaaacgc atggtgtctc tgagtaccat ccgcacccat
      721 acccaacaca tttatgccaa gctgggggta aacaaccgac gggcggccgt ccgctgcgcc
      781 gaggaactcg gtttgttgta gcaattattc acttatcatc ctaaggtacc gacagatgac
      841 tcatccctgc cggtattttt tattttcccc aaatcaataa atcatcacct aattcatcac
      901 atgtaatgat gacgcctcat cacatttacc attatctttt ggccgcaata aagaatggat
      961 gcaaacctgt agaaattcat ccaaatcgag aaaattgcac gaggcactgc atgagtgaaa
     1021 cacacgcttt acctgaatac tatgacgggt ctgaacttta cgaaattcgc ctcaaggggc
     1081 acctggatga ccggtgggcc gaccggtttg agggtctgac catcacactg gaagaaaacg
     1141 gcgatacgct tttagctggg ccagtgattg accaggctgc gttgcatggc ttgctcaaaa
     1201 aagtgcgtga tttaggaatg ctattggttt cggttaattt tgttaacccc ggtcgggcaa
     1261 aaacgcagga ggtcaaatcg taaatcatat ctggctttat gttccaaaga aataaatcaa
     1321 tatcagtaga tcacgaaata acttggagga gcccgaaaac aggggaatct tctgggcatt
     1381 tgttcacctc tcgtgaagta ctgaatatca actaacaagg agaaaaaaat gaagacatac
     1441 agaatgaatg cagtaatggc aggcgtcctc tacttcctgg ggacggtctt tggggtcttg
     1501 agcgcagtca ttgggggcga agtgctttcc tcgctcaata tcggcaaacc gcttgtcggt
     1561 atggatatgc tgggccttgt tgtcgccaat tcgtcacgaa ttaccgtggg ggcatttttt
     1621 atcttcatga tggcgatttc tctggtggcg atgacggttt tcctctaccc gatctttaga
     1681 aaagacagcg aagagctggc gctggggatg gtccttttcc gcggggcact ggagggaact
     1741 ggctatattg tttcaaccgt cggttttttg gcactggtcg tacttggcaa cgaatacatt
     1801 gccaccgggg ctgattcggc tgccttgcag tccatgggca atctcgtgta tcagttccaa
     1861 gtccgcctcg ccccgattgg tcccatcttt tttctcatag gggctatgtg tttatacctc
     1921 tccttctacc gcacccggct catcccccgc tggttaaccg tctggggcat gatcgccgtg
     1981 gttttctata tggcttccac cctgttgaaa ttctttcaca tgggcaccgg cattgggttt
     2041 tatttggaaa tgataatggc gccacaggaa atggtcatgg cggtctggtt gatcgtgaag
     2101 ggcttcaatc catctgcgat cgctatcctg tttgctaaaa cagaataacg gattgatttt
     2161 acccactgat ggactggcat tcctgaacat gatgccaggt cgcaattggc aaactctttg
     2221 ccattcttag gagactgact atgcaaaact acctgctata cgcgtgtgaa atcttacttg
     2281 tcgttgtcac gcctctgctt gtcttataca tcaagggaaa ctggccactg cgcaaggtta
     2341 tcccgcctct cgtggtcatt cccatccttt ggtattttac ttatgcgccc ttgcatgagt
     2401 tgagtcatgc cgctggactc tatttggttg gaggcaaagt cattgattac aaactcattc
     2461 ccagattctg gttgggcgaa ttcgctggtg cctggatcac accaagtgga attactcaaa
     2521 gttggcaaca attaacgatg agcggcttcc catacatgct ggacattgtc ttttttgttg
     2581 tagcgttgtt tgtctttagg cgagcttttt ccaggggtcc tttttttatt ggttttgctt
     2641 tcatgcttct atgcctcaga cctgcgtgtg actttgtatt tgaacctatt gcttttcttt
     2701 ccggcgatag aaatgatttc tacgcaatac aacagattgt cggaccctct gtaatatggt
     2761 catttatcct gatcgcaatc ggtctcgctg tatattctat tgtctcaaca ctaagccact
     2821 tggttagctt ttcaaaacag tcgattgggc aaaataccaa tgaaacaaaa cacaatatca
     2881 ttccaaaaga aagaggagat ttaaaatgaa cacctataga aagacagcca tcaccgtggg
     2941 cgcactattc atcgtctgca ccgttgccag tatccttggc cccacactgg ctatttcagc
     3001 taattccccg gactatctcg atcaactggc tggcaatcca gaccagatca tcactgcggc
     3061 tctgctggaa ttcatctggg ccgctgctgg cgctagcata gcaattgggc tgtacccact
     3121 tttgaaaaag tacaacgcag ccctcgcgct tggttccgtc ggatttcggg tcgttgagaa
     3181 tgtattcgtt ctcatcggca cgctcagcct gctgtcacta ttgacattga gccaggagtt
     3241 cattgcggca ggggcccctg aagcatcttc gtttcagact ttgggcacat tgttgttggc
     3301 ggtacgtgac tggcaactcc atgtaatatc ggggcttgcc ttttccctgg gagtcctgat
     3361 gtattacgca atattgtata agtcgaacct catcccccga tggttatcgg gctggggtgt
     3421 tctcggcgct attctggcac tggcagcaac tgtgttggct tcattcaccc gcgattttgg
     3481 gctggagtcg gctcacacct atttgcacat ccccataggc ttgcaggaac tggttttcgc
     3541 tgtctggctg atcgcgaaag gattcaatcc atctgcaatc gctgccctgt ctgcgaaaac
     3601 ag
//
LOCUS       DASTJP010000600         5985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1315558, whole genome shotgun sequence.
ACCESSION   DASTJP010000600 DASTJP010000000
VERSION     DASTJP010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315558"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /gene="msrP"
                     /locus_tag="VFQ23_17675"
     CDS             <1..579
                     /gene="msrP"
                     /locus_tag="VFQ23_17675"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HET9908484.1"
                     /translation="KFTQEERIYRLRCVEAWSMVIPWTGFPLASLLKEVEPTSKAQYV
                     RFETVLRPEEMKGQGSPFYPWPYNEGLRLDEAMNDLTILATGLYGEPMPNPNGAPIRL
                     VVPWKYGFKSIKSIVKIELTEERPQTLWNTVGPGEYGFYSNVNPDRPHPRWSQASERR
                     IGELSRRPTLPFNGYGEQVADMYAEMDLDLYY"
     gene            625..1305
                     /locus_tag="VFQ23_17680"
     CDS             625..1305
                     /locus_tag="VFQ23_17680"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HET9908485.1"
                     /translation="MNNLFKRYTPLQIAMHLYAWSAIAHILFDFITGNISPNPIQDLE
                     QRTGRHAITLLVLSLACTPLNNIFGWRELIKRRRALGLYAFMYATIHILIFVNLDYGL
                     AWSLIVQTVFEKPYIVVGLISFLMLIPLAATSFDIWKKRLGKNWKRLHQIVYLLVPLA
                     VLHYAWGKKGDFFALQGEIIRPLIYAVIVTLLLTVRIPVVRRAVASLRDRILLLLRKR
                     GTQPEANP"
     gene            1442..1975
                     /locus_tag="VFQ23_17685"
     CDS             1442..1975
                     /locus_tag="VFQ23_17685"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HET9908486.1"
                     /translation="MKQTTEKSHFIRVLARVRRLILLTALIMGITLACAGNAPATPEI
                     AGSLVPSSTSTMSVPTPTPGLDFSKTRLSDNGLFLVSYVSSKDIVPVNQIHQWTLHVE
                     TADGQLVENASITVDGDMPEHAHGMPTRPQVTEYLGNGDYLVEGVKFQMGGWWVMDFT
                     ITADNLKDAVRFNMRLN"
     gene            2028..3287
                     /locus_tag="VFQ23_17690"
     CDS             2028..3287
                     /locus_tag="VFQ23_17690"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9908487.1"
                     /translation="MKTKHLAILIVFIGIALSIYACSGIGATPWSEEEIAKLRTLWIG
                     SLPPLPVDPSNRYADDPRAAAFGQKLFFDTRFSSNGEVACGTCHLPDKLFQDGIPLSN
                     GVGTTDRRAMTVIGTAYSPWFFWDGRKDSQWAQALGPMENPVEHGSNRTYYAHLINQY
                     YREEYESIFGALPDVSHLPVLTGSVQSPEVIAAWEAMTAEDRENVTWIYVNMGKSIAA
                     YERHLIPGASRFDQYVEAVLNKDYESAHKIFSSDEVAGLRLFIGKANCTDCHNGPLFT
                     NNDFHNTGVPAVKDLPQDTGRAKGAQLVLADEFNCLSLYSDAGPDDCAELRFMVSEGH
                     QLERQFKPPSLRSVAERGPYMHAGQFATLEEVLQHYNTAPVTSADDNHSELEPLNLTS
                     EEITQIIAFLKTLDSPINADLKWVTKP"
     gene            complement(3347..3574)
                     /locus_tag="VFQ23_17695"
     CDS             complement(3347..3574)
                     /locus_tag="VFQ23_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908488.1"
                     /translation="MTTHAKHQIPWYLWPFAAIWKLLAVIVELTGRLVAMILGIVLMI
                     LGILVSLTILGAIVGIPLAIVGFLLFLKGIF"
     gene            3701..4495
                     /locus_tag="VFQ23_17700"
     CDS             3701..4495
                     /locus_tag="VFQ23_17700"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET9908489.1"
                     /translation="MPSRKKTIIDEPFVQPVSEPESISEPVIQDEDTITFKKSHFYAG
                     LTVLAFAAGILVGYVVWGTNSIAQTASQSSGQLIDAPVTAQPQYTRYEIPTEGFHSLG
                     PADAPITVVEFSDFQCPYCRRWHEETYEPLLAAYPGKIKIVYRHLPLVSIHPDAFSAA
                     EAAMCAGDQNVFWQYHDMLFSGDSLGSAVYEQYARELSLDIPTFTDCIASHKFQQAIQ
                     EDSDFAVNLGVRSTPTFFVNGLAIVGAQPLDVFKQLIDKELAGEIP"
     gene            4611..5282
                     /locus_tag="VFQ23_17705"
     CDS             4611..5282
                     /locus_tag="VFQ23_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET9908490.1"
                     /translation="MMVKKYVAVAGNIGVGKSTLVELICVRMGWEPFFEPVTENPYLA
                     DFYNNMDAWSFHSQVFFLTHRLRAHYNLTQHPTSVVQDRSVYEDAEIFAQNLYLQGHI
                     QKRDYQTYRDMYETMMRFLPPPDLVVYLRASVSTLENRIQSRGRTYERTITPDYLQGL
                     NNLYETWIDNFTLCPVLAVPADDLDYVSHPGHLRLIIDKVEEKLTGKEEVVFEPEEVA
                     RAAED"
     gene            complement(5390..>5985)
                     /locus_tag="VFQ23_17710"
     CDS             complement(5390..>5985)
                     /locus_tag="VFQ23_17710"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9908491.1"
                     /translation="RPGHDWNNDGVVNVGDEYIELINHGVIDVNLGGYILDDEVNIGS
                     TPYNLPSITISPGERIVFYGSETNLLLSDGGDGVRLLKPNGQLGDAYNYRVVRFPDQS
                     FCRLPDNGGLDDWNTNCFPTPGLQNALSGSSVSPPNVGSAEDLCPIADTLPIDFILAE
                     CPPFGNNIWRPAYWDEPGWYGEKYLPESPGKWPVFVD"
BASE COUNT         1510 a         1538 c         1465 g         1472 t
ORIGIN      
        1 aaattcaccc aggaagaacg catctatcgg ttaagatgtg tggaagcctg gagtatggtc
       61 atcccatgga ccggttttcc gcttgccagt ctcttgaagg aagtggaacc cacctcgaaa
      121 gcacaatacg ttcgctttga aacggttctt cgccccgaag aaatgaaggg acagggaagc
      181 ccattttatc cctggcccta caacgaggga ctgcgactcg acgaggcaat gaacgacctc
      241 accatcctgg ccacaggctt atatggtgag ccgatgccaa atccaaacgg ggcgcccatt
      301 cggttggttg tgccctggaa gtacggtttc aagtcgatta agtctattgt caagatagaa
      361 ttgacagaag aacgaccgca aacgctctgg aacaccgttg gacctggtga atatggtttc
      421 tattccaatg tcaaccctga tcgcccgcat ccgcgctggt cacaggcaag cgagcgccgc
      481 atcggtgaat tgagccgccg cccaaccctg ccgttcaatg gatatggtga gcaggtggcc
      541 gacatgtacg cagagatgga tttggatttg tattattaat ttttatgtag gggcaggcta
      601 accctgcccc tactacgata cagaatgaat aaccttttta agcgctacac ccctcttcaa
      661 atcgccatgc atctttatgc ctggtctgcc atcgcgcata tcctctttga tttcattaca
      721 ggaaatattt cccccaatcc cattcaggat ctggagcagc gcacaggacg gcatgcaatc
      781 acactgcttg tcttgtctct tgcctgtaca ccgctcaata acatctttgg ctggagagaa
      841 ctgatcaaac gccgccgcgc gctcgggctg tacgcgttta tgtatgccac catccatatc
      901 ctcatctttg tcaacctgga ttatgggctg gcctggagtc tgatcgttca aactgttttt
      961 gaaaaaccgt atatcgtcgt cgggttaatt tccttcctga tgctcattcc tctggctgcc
     1021 acatcgtttg acatctggaa aaaacgactc ggtaagaact ggaaacgttt gcatcaaatc
     1081 gtttatttgc tcgtcccact ggctgtattg cattatgcct ggggcaagaa gggagatttt
     1141 tttgccctgc aaggtgagat tatccgcccc ctgatctatg cggtgatcgt aactctgctg
     1201 ttgacggtac gcattccagt cgtgcggaga gcggttgcat cactgcgcga ccgaatcctg
     1261 ctactgcttc gaaagcgagg gactcagcct gaggctaatc cctgatcaaa aagatgattt
     1321 ccacctcaaa tgaggcattt tagaccgtcc ttcaggacgg tcttttgttt tcactacaac
     1381 ccaacctgct gcccatgcgt acatagattt gatgtagtat cctgtccaaa aggagcaagt
     1441 catgaaacag acaaccgaaa agtcacattt cattcgcgtc ctggcgcgag tccgcaggct
     1501 tatccttcta actgcgctga ttatgggtat cactctggca tgtgcaggga acgcacctgc
     1561 aactcctgaa attgcaggca gtctcgtgcc cagttctact tcaacgatgt ccgttccaac
     1621 ccccacgccc ggtttggatt tttccaaaac ccgcctctca gataacggct tgttcctcgt
     1681 ctcgtacgtg agttcaaagg atattgtccc ggttaaccaa atccatcagt ggacactgca
     1741 cgttgaaaca gcggatggcc agctggttga gaacgcctct attactgtgg atggtgacat
     1801 gcctgagcac gcacatggta tgcccactcg tccacaagtt accgaatatc tgggtaatgg
     1861 agattatctc gtcgaaggtg taaagttcca aatgggtgga tggtgggtga tggactttac
     1921 gatcaccgca gacaatctca aggatgcagt gcgcttcaac atgaggttaa attaaatata
     1981 gacgatcact gtcgaaggga ccgccagggt ctataaaaag aaatagtatg aaaaccaagc
     2041 atcttgcaat actcattgtg tttattggca ttgcattaag catttatgcc tgctctggca
     2101 ttggggcgac tccctggagt gaggaagaaa tagcaaaact tcgcacgctt tggatcggaa
     2161 gtttgccacc gttgcctgtt gatccatcca accgatatgc agacgacccg cgcgctgccg
     2221 cattcggtca aaaacttttt ttcgacacgc gctttagttc aaatggagaa gtcgcctgtg
     2281 ggacatgtca tctgccggat aaacttttcc aggatggcat cccactctca aacggagtcg
     2341 gcaccaccga ccgccgcgcg atgacagtga ttggcacagc atatagtcca tggttcttct
     2401 gggatggacg caaggatagt cagtgggcac aggcgctggg tccgatggaa aacccggtag
     2461 agcatggcag caaccgtact tactacgcgc atcttatcaa tcaatattat cgcgaggaat
     2521 acgaatcgat cttcggcgcg ctgcctgatg tgagtcattt gccagttcta acgggatccg
     2581 ttcaaagtcc ggaggtgatt gccgcctggg aggcgatgac ggccgaagac cgtgaaaacg
     2641 tcacgtggat ttacgtcaac atgggcaaat ccattgcggc ttatgagcga catctcatcc
     2701 ctggcgcgtc gcgctttgat caatatgtgg aagcagttct aaacaaagac tatgaatcag
     2761 cgcacaagat cttctcgtct gacgaggtcg ccgggctaag gctttttatc ggcaaagcaa
     2821 actgtacgga ttgccataac ggacccttgt tcaccaacaa tgactttcat aataccggcg
     2881 tgccggctgt gaaggattta cctcaagata cggggcgtgc caaaggcgca caactggtat
     2941 tggcagatga attcaactgc ctgagcctct acagcgacgc gggacctgac gattgtgctg
     3001 aattgcgttt tatggtttca gaaggtcatc aacttgaacg ccagttcaaa ccgccttccc
     3061 tgcggagcgt ggcagaacgg ggaccgtaca tgcatgcagg tcagtttgct acgctggaag
     3121 aagtacttca gcactacaac actgcaccag taacgagcgc agatgataat catagtgaat
     3181 tggaaccatt aaatcttacc tcagaagaga taacacaaat tatcgctttc ctgaaaacat
     3241 tagatagccc catcaatgca gatctaaaat gggtaacgaa accataacct ttgagtgaat
     3301 aaaaagaaat cgctggtcga cataaaccga ccagcgattt cttttcctag aagatccctt
     3361 tgaggaataa gagaaagccg acaatcgcaa gcggaattcc tacgatcgct cccaggatcg
     3421 tcaggctgac gaggattcca agaatcatca acacaatccc gaggatcatc gccacgagcc
     3481 gtccggtcaa ctcgacgatg acggccagca atttccagat cgctgcgaac ggccagagat
     3541 accagggaat ttgatgcttc gcatgtgtag tcatttctac ctccatttta ttcgtaggtg
     3601 acagtcatcg tagagacgac tgtcaccttt catgtttcat acggcttttc aaggaataag
     3661 ttgcggagtg tcaatttttc cgcatgttat aatgccgtcc atgccctcca gaaagaaaac
     3721 aataatagat gagccctttg tccaaccggt cagtgagcct gaatcgatct ctgagccagt
     3781 aattcaggac gaagatacca tcacgttcaa gaaaagtcac ttttatgcag ggctgactgt
     3841 cttagctttt gctgccggca tccttgttgg gtatgttgtt tggggaacaa atagtattgc
     3901 ccagacggcg tcacaatcct caggacaatt gatcgatgca ccagttaccg cgcagccgca
     3961 atatacgcgt tatgagatac ccaccgaagg tttccattcc ctgggtcctg cggatgcacc
     4021 gatcaccgtt gtcgagttca gtgattttca atgtccctat tgccgccgct ggcatgaaga
     4081 gacctatgaa cctctgcttg ccgcataccc tggaaagatc aaaatcgttt atcgtcatct
     4141 gccattggtc tcgatccatc cggatgcctt ctctgccgca gaagcagcca tgtgtgcggg
     4201 cgatcagaac gtcttctggc aatatcacga tatgttgttc agcggcgatt cgttggggag
     4261 cgcggtctat gagcaatatg cccgcgaact cagcctcgat atcccgacat ttacagattg
     4321 catcgcttca cacaaattcc agcaggctat tcaggaagac agcgatttcg cggtgaacct
     4381 gggtgtacgc tcgacaccaa cgttcttcgt caacggattg gcgatcgttg gcgcacagcc
     4441 actggacgta ttcaaacaat tgatcgataa agaactcgcc ggagaaattc cgtaaattct
     4501 gtcattgcga gccccgagcg tgagcaagtg agcgaagcaa tcccaacctt ataaggagat
     4561 tgcttcgtcg cgccggtccc ctctcgcaat gacaggataa ttttggaggg atgatggtaa
     4621 aaaaatatgt agcagtcgcc ggaaatatcg gtgttgggaa atccacactg gttgaattga
     4681 tatgtgtgcg gatgggctgg gaaccgttct ttgagcctgt gacggagaat ccgtaccttg
     4741 cagattttta caataatatg gatgcctggt cgttccattc acaggtcttt ttcctgaccc
     4801 atcgtctgcg cgcgcactac aacttgaccc agcatcctac gtccgttgtt caggaccgca
     4861 gtgtgtatga ggatgccgag atctttgcac agaacttata tttacaggga cacattcaaa
     4921 agcgggatta tcagacgtat cgcgatatgt acgaaacgat gatgcgcttc cttccgcccc
     4981 cggacctggt cgtttatcta cgcgcgtccg tctcgaccct ggaaaaccgc atccaaagcc
     5041 gcggtcgcac ctacgagcgg actatcactc ccgattatct gcagggactc aacaatctct
     5101 acgaaacctg gatcgataat ttcacccttt gcccggtgct ggctgtcccc gcggatgatc
     5161 tggattatgt ctctcatccg ggtcatttgc gattgatcat cgacaaagtg gaagagaagt
     5221 taaccggtaa agaggaagtg gtctttgaac ccgaagaagt ggcacgcgcg gcagaagact
     5281 aaaacccttt atccacgaag atcacaaaga agaacttatt cttcgcgccc ttcgcgtgct
     5341 tcgcggtaaa ttttgcgtcc atctccttcg ggagagggtg ggggatttct taatctacaa
     5401 acacgggcca cttgcccggg ctttcaggga ggtatttctc tccataccag cctggctcat
     5461 cccagtaggc tggccgccag atgttattcc caaagggcgg gcattccgcc aggataaagt
     5521 cgatcggaag cgtatccgcg atagggcaaa ggtcttccgc actccccaca ttcggcgggc
     5581 tgacggaact cccgctgagt gcgttttgaa gcccgggtgt ggggaaacaa ttggtattcc
     5641 agtcatccaa accgccatta tccggcaggc ggcaaaagga ctgatctggg aaacgcacga
     5701 cgcggtagtt ataagcatca cccaactgtc cattcggttt gagcaagcgg accccatcac
     5761 cgccatcgct cagcaggagg tttgtttcgc ttccataaaa gacaatgcgc tccccgggtg
     5821 aaattgtaat ggaaggaagg ttatatggcg tggaaccgat attgacctca tcatcgagga
     5881 tatagccgcc taaattcaca tcgattacgc catggttgat gagctcgatg tattcatctc
     5941 ccacattaac gacaccatca ttgttccaat catgacccgg gcgag
//
LOCUS       DASTJP010000601         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_681930, whole genome shotgun sequence.
ACCESSION   DASTJP010000601 DASTJP010000000
VERSION     DASTJP010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681930"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /gene="cas4"
                     /locus_tag="VFQ23_17715"
     CDS             <1..491
                     /gene="cas4"
                     /locus_tag="VFQ23_17715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HET9908492.1"
                     /translation="LFLAILFFWQSNRQRQSAGLPGGRVIYTDTRGWGKLEKPLFYAA
                     LELTGKPDYLVQQNGQIIPVEVKSGRAPEAPYDSHIYQLASYCLLVEKTYGKRPPYGI
                     IHYENRDFAVDYTQELESSLLDLLAEMKRDQHKKDVPRSHEQVARCARCGFRKACDQK
                     LP"
     gene            565..1428
                     /locus_tag="VFQ23_17720"
     CDS             565..1428
                     /locus_tag="VFQ23_17720"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET9908493.1"
                     /translation="MPEAFIPKRPVVTAHQKMSEAFAEAEMMSAYFRERGVEAPYGSI
                     YDEDLRKRVRHGEFDMLIVAGGDGSVLRAGHLCAPSGVPILGVNLGRIGFLIQVERQE
                     WREYFDKLFNGEAWIENRMMLHVEHVRAGEILASWNALNEVVVARGHMLRPVRLTTSV
                     DGRQLISYLADGLIASTPTGSTAYALAAGGPILPPEIRNILLVPIAPHLSVDRAIVLS
                     EGAVVKISNRGENAVLSLDGQPPIPLLEDDLVTACAADVTTRFVRFGDPGYFYRNLTA
                     HMNENSLGFSR"
     gene            1425..1946
                     /locus_tag="VFQ23_17725"
     CDS             1425..1946
                     /locus_tag="VFQ23_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908494.1"
                     /translation="MTESTTLYCYVHPNRETSLRCNNCNRPICASCAVRTPTGYRCRE
                     CVRERQKTFETSEWYDFVSGFIVAAILSGVAAFLVTLIGGIGFFGWFLIAAGAPTAAV
                     AIAEGVRVVTKRRRSRGLFMTVVAGVVLGALPVILFQAATQNFFGIVFQVIYLIIATP
                     IVYTRLSGIQLFR"
BASE COUNT          449 a          506 c          528 g          538 t
ORIGIN      
        1 ttctatttct tgctattctc ttcttctggc aatcgaaccg ccagcggcag tctgcagggc
       61 tccccggcgg gcgcgtgatc tacacggata cgcgcggctg gggcaaattg gagaaacccc
      121 tgttttacgc cgcactggaa ttaacgggca agcctgatta tctggtgcaa caaaatggac
      181 aaatcatccc cgtcgaagtg aaatcgggac gcgcacccga agctccgtat gactcgcata
      241 tttatcaact ggcatcctat tgtttgctgg tcgaaaagac ctatggcaaa cgaccgcctt
      301 atgggatcat tcattatgag aaccgcgatt ttgcagtgga ttacactcag gaattggaat
      361 catctctcct tgatttgctt gccgaaatga aacgcgatca gcataagaaa gacgtcccgc
      421 gttcccatga acaggtcgcc cgttgtgccc gctgcggatt tagaaaggcc tgtgatcaaa
      481 agttaccata gacattagtg gcacgattaa tccaaaataa ctttcgtttt gcggtgcccc
      541 tacaaaaggt ataatttgtc taccatgccc gaagccttta ttccaaagcg accggttgtc
      601 actgcgcacc agaagatgtc tgaagccttc gcggaagcag agatgatgtc agcgtatttc
      661 agggaacgag gcgtcgaagc gccctatggc tcgatctatg atgaggattt gcgcaaacgg
      721 gtccggcatg gtgaatttga catgcttatc gtcgcaggcg gtgacggcag cgttttgcgg
      781 gctgggcacc tctgtgcccc aagcggcgtg ccaattctag gcgtgaacct cggacggatc
      841 ggcttcctta ttcaggtgga acgccaggaa tggcgtgagt actttgataa actattcaat
      901 ggcgaagcct ggattgaaaa ccgcatgatg ctgcatgtcg aacatgttcg tgcgggtgag
      961 atcctggcaa gctggaatgc gttgaatgaa gtggtcgtgg cgcgtgggca tatgcttcgg
     1021 cccgttcgat tgacgacgtc ggtggatggt cgtcaattga tcagttatct tgctgacggg
     1081 ctcatcgctt cgactccgac cggttcgacg gcgtatgccc tggccgcagg cggaccgatc
     1141 ctcccgcctg aaatccggaa tattttgctc gtgccgatcg cgccgcatct ctctgtagat
     1201 cgagccatcg ttttatccga gggtgccgtc gtaaagatca gcaatcgagg cgagaacgca
     1261 gtccttagtt tggatgggca acctcctatt ccattgcttg aagatgacct tgtgactgca
     1321 tgcgcggctg atgtcactac acgatttgta cgatttggag accccggata tttttatcgc
     1381 aacctgactg ctcatatgaa tgaaaactct ttaggatttt cgagatgacc gaatcaacga
     1441 ctctatattg ttatgttcat ccaaaccgtg agacctccct gcgatgtaat aattgcaatc
     1501 gtcccatttg cgcgtcctgt gcagtccgta cgccgacggg gtatcgttgc agggaatgtg
     1561 tccgtgaacg ccagaagacc tttgaaacgt cagaatggta tgactttgtt tcgggcttta
     1621 tcgttgcagc gatactttct ggagttgctg cttttctggt cacactcatt ggggggatcg
     1681 gctttttcgg ctggttcctg atcgccgcag gcgcgcccac ggctgccgtg gcaattgccg
     1741 aaggcgtccg tgttgtgaca aaacgccgac gttcacgcgg gttgtttatg accgtggttg
     1801 ccggtgttgt gttgggggcg ctgcctgtta ttttatttca ggcggccacc caaaactttt
     1861 tcggcattgt ctttcaagtg atctatctca tcattgccac ccccattgta tatacccgac
     1921 tttctggaat ccaattgttc agataaccaa taacctatgc ttaccgaact ccatatacaa
     1981 aattttgcga tcatcgataa actcgactta agatttggtc c
//
LOCUS       DASTJP010000602         5975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1210006, whole genome shotgun sequence.
ACCESSION   DASTJP010000602 DASTJP010000000
VERSION     DASTJP010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210006"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFQ23_17730"
     CDS             complement(<1..363)
                     /locus_tag="VFQ23_17730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9908495.1"
                     /translation="MFNFFTKSSDTNAATANDHAMIDLQAVHKSYKTAVGDYPALKSI
                     DLQINAGEFVSIIGKSGSGKSTLLNMITGIDRPTLGAVHVNGTAVHEMNENRMARWRG
                     NNLGIVFQFFQLLPMISVI"
     gene            complement(398..1432)
                     /locus_tag="VFQ23_17735"
     CDS             complement(398..1432)
                     /locus_tag="VFQ23_17735"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9908496.1"
                     /translation="MKARIISLFVLAGLVFTGCSAGGTAATETTPIPTVLADEALIAE
                     GRIEPVRYAEIAFNVSGVIGEVLVTEGQAVRAGEPLIQLGNESDRDYAAAQLELVTAQ
                     QELDNLFNSSGGELAQAVINLKDAQEAFEKADNYLIYLQNSRKVPQTETRVFLIQTFK
                     GYEYQYKTKNFKGPAPEDWIIEAENDLAVKKAALDDAQRAYDRLKDGVDSDQRTLLEA
                     RLNAAKAGVAAFTVVAPFDGTVADLPAQQGSSINVGDVAVTIADFSHWIVKTTDLTEL
                     DVVTVQDGQPTVITLDAIPDVALNGEIQSISQTFEEKQGDVVYEVTVALQDGHPSLRW
                     GMTAIVTFDE"
     gene            1953..2735
                     /locus_tag="VFQ23_17740"
     CDS             1953..2735
                     /locus_tag="VFQ23_17740"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HET9908497.1"
                     /translation="MSTNRQFSLALEALAELKTDASTPAANLTPVAPTLGEILEEIGT
                     LPREALLLGVASDGLPILLNLYDSHPGPLIVLGDSGAGKTNFLQTVAKSAIQTHHPND
                     LQYGVITNHIEEWEIIEETPHSAGVFYVGHPNAQDLILSLATWAHTNKNTRQSVLLLV
                     DGLEDVANLEADVVQSFRWLLLRGPSRRVWPIITLQAENYEQVVSWLQNFRTRIFGHI
                     ANEHIAWALGADKVSALNQLEPRIQFSLPENGNWLRFWLPSC"
     gene            2926..3255
                     /locus_tag="VFQ23_17745"
     CDS             2926..3255
                     /locus_tag="VFQ23_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET9908498.1"
                     /translation="MEAKLYVGNLPFSTGDADLQNLFAQAGNVKSAQVIKDRASGRSK
                     GFGFVEMSSPDEAQGAITMFHGKDFNGRALTVNLARPREERPGGFRSGGGRRQSGHDG
                     GERRRDY"
     gene            3359..3814
                     /locus_tag="VFQ23_17750"
     CDS             3359..3814
                     /locus_tag="VFQ23_17750"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908499.1"
                     /translation="MSQSSSIREYRSDDFDVVTILWRISREKSLPEFQMEKGHFFYED
                     RNYFHNFVLKNNKVWVVESNNRPVAFMAMNKDFIDQLYIDPDYQRRGIGQSLIDFARE
                     QSPDHLWLYTLQINVNARSFYEKNGFVAEKFGVSPPPESEPDVEYHWRK"
     gene            3817..4908
                     /gene="alr"
                     /locus_tag="VFQ23_17755"
     CDS             3817..4908
                     /gene="alr"
                     /locus_tag="VFQ23_17755"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9908500.1"
                     /translation="MRPTYLEVNLPQLQKNIESIRAHINSARLMAMVKANAYGHGLDG
                     VGPFIEPYVDYFGVALLEEGIQLRELGVRKPILVAGGTIPEQIPAFIEHNLILTASSP
                     HLLAAADAAAISAKKKLVVHLKIDTGMERVGVHYYDAEQFLEQSLKFKNIEIEGIYTH
                     LANSEDPTLNHARQQLERFQNVLQFYEKRNVPTPLRHVANSGAILQFPESYLDMARPG
                     LILYGVYPAGVPHTVDISPAIRWRSRVAYSKITRPGHPVSYGSLWQAETETRIATIPC
                     GYADGYFRRMTNQAEVVINGKKYPQVGRICMDQFMVNVGDDVAEVGDEVTLLGDGITA
                     YDLADWTGTNEYEVLTNISARVPRLYLKE"
     gene            4945..5481
                     /gene="lspA"
                     /locus_tag="VFQ23_17760"
     CDS             4945..5481
                     /gene="lspA"
                     /locus_tag="VFQ23_17760"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET9908501.1"
                     /translation="MKSVLQRYWPIATIAALIVALDQWTKWLVRVNIPDGGVWLPESL
                     AWLTPYARIVHWHNTGAAFGMFQNASMVFTVLAFIVIAAILYYYPHVENSDWSLRLAM
                     SMQLGGAVGNLIDRLTQGRVTDFISIGTFPVFNVADASISVGAVVLLLGVWLTERAER
                     QEKARLMSADQAGETTSD"
     gene            5474..>5975
                     /locus_tag="VFQ23_17765"
     CDS             5474..>5975
                     /locus_tag="VFQ23_17765"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9908502.1"
                     /translation="MTDRVVQFLFDKTTPERLDKFLVEKLQEFSRSRIQALIADGFVD
                     VDERAAKKAGQLLENGFNVVVRIPPPMPTDLVAESIPLDILFENDDLIVVNKPAGMVV
                     HPAAGHASGTLVNAVLGYDPDLEGIGGEERPGVVHRLDKETSGLILLARNERAHRWLQ
                     DQFRLRK"
BASE COUNT         1501 a         1369 c         1522 g         1583 t
ORIGIN      
        1 gatgacagag atcatcggca gcagttgaaa aaactggaat acgattccca ggttatttcc
       61 acgccaacgc gccatccggt tttcattcat ctcatgaacg gcagttccat tgacgtgcac
      121 tgcgcccagc gttgggcgat caataccggt aatcatgtta agcagcgttg atttcccgct
      181 gccagatttg ccgataatgc tgacaaattc gccggcgttg atctgtagat caatgctttt
      241 tagcgcggga tagtcgccga cggcggtctt atacgattta tgcaccgcct gtaaatcaat
      301 catggcatga tcgtttgcag ttgctgcgtt tgtgtcactt gactttgtaa aaaaattgaa
      361 catcgattac tcccaatccg cgcagatcag gcaagcccta ttcatcgaat gtcacaattg
      421 ctgtcatacc ccaacgcagg cttggatgtc cgtcctgcaa agcaactgtc acttcataga
      481 ccacatcccc ttgtttttcc tcaaaggtct ggctgatgga ttgaatttct ccgttcaatg
      541 caacatcagg aatcgcatcc agggtaatga ccgttggctg accgtcttgc actgtcacga
      601 catccaactc agttagatca gtcgtcttga cgatccagtg tgaaaaatca gcgatcgtca
      661 cagctacatc accgacatta atggaacttc cctgttgcgc gggtagatct gcgaccgtcc
      721 catcgaacgg ggcaacgaca gtaaaggccg ctacgccggc tttcgcggca tttaagcgtg
      781 cttccaacag agttcgttgg tccgaatcca cgccgtcctt gagacgatca taggcgcgct
      841 gagcatcatc cagagcagcc tttttcaccg ccagatcatt ctctgcttct atgatccaat
      901 cttcaggagc aggacctttg aaattcttgg tcttgtattg gtattcatac cctttgaagg
      961 tttgaatgag aaatacacgg gtttccgttt gagggacttt cctggaattt tgcaaataga
     1021 ttaaatagtt atcagccttc tcgaatgctt cttgcgcgtc tttgaggttg attaccgcct
     1081 gtgcaagctc gccgcccgac gagttgaaca gattatccag ttcctgttga gcggttacca
     1141 attcaagttg cgcggcagca taatctctat ctgattcgtt tcccaattgg atcaacggtt
     1201 cgcccgctcg tactgcctgt ccctctgtca ccaacacttc tccaataact ccgctgacgt
     1261 tgaaagcaat ttcggcatac cggaccggtt cgatgcggcc ttcggcaatg agtgcttcgt
     1321 ctgcgagtac agttggaatg ggtgtggttt ctgttgctgc agtgccgcct gcggagcaac
     1381 ctgtgaatac caggcccgcc aaaacaaaaa gcgaaatgat tcgtgccttc atacgtatac
     1441 cctgaccttt ccctgaaatg attatctgct acatctacga cctgcaaagg cataagttgc
     1501 agatgtccac actcatattg gaggaatcgc cagaaaaggt gtcctgcaaa ctattctaac
     1561 cgcccacgcc taaaaaggta tagccttcaa gagtatatag atacgcttca taagtagggg
     1621 agccggtgct tctaacctct atgaattaaa gaaagctgtt gggtatgaag aagacggggt
     1681 aaatggagcg caacaccgat ggagtaatga gggcgattct acgcgcctgg agataacttc
     1741 agatacatac aaatcgtcca ttgcactgcg agcattcgaa atcgaatctt taagtcacca
     1801 tactcttgac gaatatcgcg gcgtggttaa aatagaacat acgttctaat taaaaagagt
     1861 gccgcagcca gtccaagtct gagccttggt tgttgatctg aattacttca tgtgcccaga
     1921 cgtgaaaacg gtgagcagca aaaggaggac gtatgtcaac caatcgtcaa ttctctctgg
     1981 ccctcgaggc gttagccgaa ctaaaaacag atgcgtcaac accggctgcc aatctgaccc
     2041 ccgtcgcacc aaccctgggt gaaattctgg aagagatcgg cacgctgccg cgggaagctc
     2101 tgttgttggg tgtggcatca gatggactgc ccattctgct gaatctttat gattctcatc
     2161 ctggtcctct catcgtgctg ggggactcgg gcgcgggtaa aacaaatttt ttgcaaaccg
     2221 tggcaaagtc cgctatccag acgcatcatc ccaatgattt gcagtatggt gttatcacga
     2281 atcacatcga ggaatgggaa atcattgaag agactccgca tagtgccggc gtcttctatg
     2341 ttggtcaccc gaacgcgcaa gaccttatcc tttcgctggc aacctgggca cacacaaata
     2401 aaaatacgcg tcaatctgtg ttgttattgg ttgatggatt ggaagatgtg gcgaaccttg
     2461 aggctgacgt ggttcaaagt tttcgctggc tattgttgcg tggtccttca cggcgggtat
     2521 ggccgatcat caccttacag gctgaaaact atgaacaggt ggtctcctgg ctgcaaaact
     2581 tccgaacgag aatctttggt catattgcga atgaacatat cgcatgggcg cttggcgccg
     2641 acaaagtttc agcgttaaat cagctggagc cgcggattca gttttcgctt cctgagaacg
     2701 ggaactggct tcgattttgg ctacccagtt gctagtcgca gtatccttct tgtgctacaa
     2761 tcgaaatgtt agttgcatgg atggctggtg gtccgacaat cagagggaat tgttccgctt
     2821 cttggcacct gggtgtctgt gatcgctgta gacatgcata atcgacccat ttcgccacga
     2881 gaaatgcttc aatttgtggc ccacactcag gagaagaata gatctatgga agcgaaactg
     2941 tacgttggca atttgccgtt cagcacgggt gatgcagatt tgcaaaactt gttcgctcaa
     3001 gcaggaaatg taaagtcggc ccaggttatc aaagatcgcg caagtggacg ctcgaagggg
     3061 tttggtttcg ttgagatgtc ctcccctgat gaagcccagg gcgcaattac catgtttcat
     3121 ggcaaggatt tcaacggtcg tgcacttacc gtgaaccttg cacgaccacg tgaagaacga
     3181 ccaggggggt tccgcagtgg aggcggacgc agacaaagtg gtcacgatgg tggcgagcgc
     3241 aggcgcgatt actaaaatta ccttcaaatt taaatgacta tatgagccaa atgggcggtg
     3301 ccaaccattt ggctcaattt atttttctga atattggtgg atattacgga gcacaagaat
     3361 gtctcaaagt tctagcattc gagaatatcg gtcagacgat ttcgacgtgg tgacaatatt
     3421 atggcgcatc tcgcgtgaaa aatctctccc cgagtttcaa atggagaaag gccatttttt
     3481 ttacgaagat cgcaattatt ttcataattt tgttctaaag aataataaag tctgggttgt
     3541 tgaatcgaat aatcgaccgg ttgcttttat ggcgatgaat aaggatttta ttgaccagtt
     3601 atatattgac ccggattatc agcgacgggg gatcgggcag tctttaattg atttcgcgag
     3661 agagcaatct ccagatcatt tatggcttta caccttgcag atcaacgtta atgcgcgctc
     3721 gttttatgag aaaaacggct tcgtagccga aaagttcgga gtaagtccgc cgcctgagag
     3781 cgagccagat gtggaatatc attggcggaa gtagtcatgc gtcccaccta tcttgaggta
     3841 aatctgcctc aactacaaaa aaatattgaa tccattcgcg cccatattaa ttcggcgcga
     3901 ctcatggcga tggtcaaggc aaatgcatac ggtcatggtc tggatggtgt tggaccgttt
     3961 attgaacctt atgtggatta ttttggtgtt gctctgctag aagagggaat ccagttacgt
     4021 gagctcggag tcagaaagcc gattctggtt gcaggaggga caattcccga gcaaatccca
     4081 gcgtttatcg agcataattt aattctcaca gcttcatcgc cgcatctact tgccgcggcg
     4141 gacgcggcag ccatttctgc gaaaaagaag ctggtcgtcc atctgaagat cgatacaggg
     4201 atggagcgag tcggagtaca ttattatgat gctgagcaat tcctcgagca atcgcttaaa
     4261 ttcaagaaca ttgaaatcga gggaatttac acccatctcg caaattcaga agacccaact
     4321 ctcaaccatg cccgccagca gctagagcga tttcaaaatg tcctgcagtt ttatgaaaaa
     4381 aggaatgtac cgactccgct acggcacgtc gcaaattcgg gagcaatttt acagtttcct
     4441 gaaagctatt tggatatggc gcgtcccggc ttgattcttt atggagtcta ccccgctggc
     4501 gttcctcata cagtggacat ctcgcctgcg atacgatggc gttccagggt agcctattca
     4561 aagatcacgc gaccggggca tccggtcagt tatgggtcgt tgtggcaggc cgagacagaa
     4621 acccgaatcg cgacaattcc atgtgggtac gccgacggat acttccggcg tatgacgaat
     4681 caagcggaag tcgttattaa tggtaaaaaa tatccgcaag tggggcggat ttgtatggat
     4741 caatttatgg tcaatgtcgg ggatgatgtc gccgaggtgg gtgatgaagt tacattatta
     4801 ggggatggca tcacggctta tgacctagcc gactggacag gcacgaacga atatgaagta
     4861 ttgacaaaca tcagtgctcg agtgccgcgt ttgtatctta aggagtaatg gtagaatgga
     4921 gcgattttgc tttcgaggtg aagtgtgaaa agtgttctac aacgatattg gccaattgca
     4981 acgattgcgg cattgatcgt cgcattggat cagtggacaa agtggctggt tcgggtcaac
     5041 atacctgacg gtggtgtttg gcttcctgaa tcattggcct ggttaacccc ttatgcacgc
     5101 atcgttcatt ggcataatac gggtgccgca tttggcatgt tccagaacgc cagtatggtt
     5161 ttcacggtgc tggcatttat tgtgatcgca gccattcttt actattatcc gcatgtagaa
     5221 aactcggatt ggtctctgcg cctggcgatg agtatgcagt tgggtggtgc agttggtaac
     5281 ttaattgatc gtcttaccca gggccgggta acggatttta tttccatcgg gactttcccg
     5341 gttttcaatg ttgcagatgc gagtattagt gtaggtgcag ttgtattatt actgggtgtg
     5401 tggctcacag aaagagccga gagacaggaa aaggcacgcc tcatgtctgc tgaccaagcg
     5461 ggtgaaacca ccagtgactg atcgcgtggt acagtttctt ttcgataaaa caactcctga
     5521 gcggctcgat aaatttctgg tcgagaagct gcaggagttt tcgcgctcac gaattcaagc
     5581 attgatcgca gatggttttg tagatgtaga cgagcgagcc gcgaaaaagg ctggtcagtt
     5641 gctggaaaat ggattcaatg tggttgttcg gattccgcct cccatgccta cagatttggt
     5701 ggctgaatca attcctctcg atattctgtt tgaaaatgat gatctgattg tggtgaataa
     5761 accggctgga atggtcgtgc atccggcagc aggacatgcc agcggaacac tggtcaacgc
     5821 ggtcttagga tatgatcctg accttgaagg cattggtggg gaagaacgtc ctggcgttgt
     5881 ccatcgcctg gataaagaga cctctggttt gattctgctt gctagaaatg aacgcgccca
     5941 tcgctggctc caggatcagt ttcgtttgcg aaagg
//
LOCUS       DASTJP010000603         5694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_834788, whole genome shotgun sequence.
ACCESSION   DASTJP010000603 DASTJP010000000
VERSION     DASTJP010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834788"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..1635)
                     /locus_tag="VFQ23_17770"
     CDS             complement(337..1635)
                     /locus_tag="VFQ23_17770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908503.1"
                     /translation="MTSSADSAIHLANLSKLYKVYNRPADMVWELVSGRPLYKPFWAL
                     QDISFDVQRGQVVGLIGRNGAGKSTLLKIIAGTLDASSGEIQVNGRISSILELGTGFN
                     PQYSGRENIYMGGLIAGLSSEEIKEKEDWVIEFSELREFIDQPFRTYSSGMQARLTFS
                     TAVCIDPDILIIDEALSVGDARFQRKSFGKIEEFRKVGKTILFVSHDMNSIRGICDMA
                     ILLEKGKILEVGKPDPIAKKYWKILYVDQNPRQDGKTPESAADGGKPETKEKQSLVPL
                     SSPVNNPRLLRAGSREGAEVLDIGILDSEGERVTVLESGEKYSIFIKAFFKEDIEDVR
                     FGFHIVNEKGVVMYGISTSEAGIQIPPQKKGQILEARLNVTMWLTNGTYLLSGGVGIE
                     ATPLDFLYDALVLTIPRLPGIQHASVVNLQPEFEFANTTP"
     gene            complement(1632..2468)
                     /locus_tag="VFQ23_17775"
     CDS             complement(1632..2468)
                     /locus_tag="VFQ23_17775"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908504.1"
                     /translation="MYSVLNPSSNFQAFLELIALFRRRGRLIYELTKREIGERYTGQV
                     FGVLWAIGHPLLLTFVYIFIFGFVFRARTGGTIDMPLDYTAYILSGIIPWLMFQEALA
                     KASTVIIANANIVKQVIFPLEVLPVKSVLATIVTEVIFLFLLAIYTLITNGAIPWTYV
                     FLPVLILFQTLGMIGISYLFSSIGVYFRDLKDFVQIFLSIAFFILPILYLPESIPAAV
                     RPILYLNPISYMIWCFQDILYFGHFAHPWAWAIFLFGSVFIFIFGFRVFRRLKTMFGN
                     AL"
     gene            complement(2476..2976)
                     /locus_tag="VFQ23_17780"
     CDS             complement(2476..2976)
                     /locus_tag="VFQ23_17780"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET9908505.1"
                     /translation="MRIQKLNAHPMEDIKQHEYDLLNEIAQDSKVTQASLSHRLGIAV
                     GSVNWYIKRLIHRGWVKVSHLDRTRLQYDLTPEGMSVFTQRAMSYARDSLKFYGSLRN
                     KAKEIVSELKQREITTVFLEGSDEIMDIMRLTCIENGLSLSPSPNGTVVKADGQGFKI
                     IHQEKA"
     gene            complement(3005..3868)
                     /gene="rfbA"
                     /locus_tag="VFQ23_17785"
     CDS             complement(3005..3868)
                     /gene="rfbA"
                     /locus_tag="VFQ23_17785"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET9908506.1"
                     /translation="MKGIILAGGKATRLYPLTLAISKQILPVYDKPMIYYPLSMLMLA
                     GIREILVISTPDALPAFRDLLKDGSQWGLKFEYAEQGEPRGLADAFLVGREFVGGSPV
                     CLILGDNIFYGQGIPSLLQECALLKEGAIIFGYKVNDPHRYGIVEFDEQYNVLSLEEK
                     PATPRSKYAVPGIYFYDNKVVQAAESVKPSARGELEITDLNKIYLEQGTLKAKVFGRG
                     VAWLDAGTHESLLKASTFIEAIQERQGLMISCPEEIAYRMRYISLDQLRELGKSLAGN
                     SYGQYLLNLTR"
     gene            complement(3865..4986)
                     /gene="rfbB"
                     /locus_tag="VFQ23_17790"
     CDS             complement(3865..4986)
                     /gene="rfbB"
                     /locus_tag="VFQ23_17790"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET9908507.1"
                     /translation="MADKKTVIVTGGNGFIGPNLVRQLIHSRTYQVVNLDALTYAANA
                     LSLADLEGNQDYVFVKGNITDGKLVESLFAQYKPAGIFHLAAESHVDRSIVSPEEFIQ
                     TNVVGTYRLLEAARAYYSTLPEDLKRDFRFLHVSTDEVFGSLGEDGYFTEETPYAPNS
                     PYAASKASSDHFVRAYHHTYGLPTIITNCSNNYGPYQFPEKMIPLMILNALSGKSLPV
                     YGDGSNVRDWIYVEDHCKAVQLAFESGKVGGEYAIGANNEQKNINLVNEICSILDDIA
                     PPVEIPQLNERGLKSYRELIQFVKDRPGHDRRYAIDSARITRELGWHPEVRFERGLRK
                     TIEWYLSNPKWVDQASGGSYGEWIEKNYAWRADAQRESK"
     gene            complement(4998..>5694)
                     /locus_tag="VFQ23_17795"
     CDS             complement(4998..>5694)
                     /locus_tag="VFQ23_17795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9908508.1"
                     /translation="IRVAVSAYTRELAQKNYPEEILVIPNGVDVKAVRPERIQVNTPP
                     VIVFAGRLMPQKAPLQLVQSLKEITDIPWRCVIIGDGTLLPDLKKWIIEANLEDRFLL
                     TGWITPQEVMQWFDRSDILFMPSLSEGLPVVGVQALAKGLAIVASRVGGFVDLVDDAQ
                     NGYLVERSAISHFSLLLRNLLEDPNRLLSFRNASLEKAKSFEIDRIVDQYENIFLGII
                     RQKSDLVETKFEI"
BASE COUNT         1542 a         1428 c         1263 g         1461 t
ORIGIN      
        1 aaggatgtaa aaagataaag ttgcagaagc tcttaatggt ttttaacatg tgcaaagaga
       61 attgtatctc cacgatagat tgtgaaatgg tctaggtaaa actcatagtt actatctaat
      121 tgggcatagt gagtgcttgc tattagaaaa agatctctta aaacagacta ctaagtgttt
      181 gaactttgta tattcccaat ctccaataaa ctctccatgc ggcaagagtt cagaattcca
      241 attttctgaa ccataccaat taggaagact attgattatt tcttttccca aggctacaaa
      301 aaaatgatcg tgggcttttt tcatcttctt aagtttctac ggtgttgtgt tagcgaactc
      361 aaactctggc tgtaggttaa ctactgaagc atgctgtatt cctggcaagc gtggaattgt
      421 caacaccagt gcatcgtaga ggaaatcaag tggtgtggct tcaatcccaa cacctccact
      481 aagtaaataa gttccatttg ttaaccacat ggtgacattt agtcgtgctt ccagaatctg
      541 accttttttt tgtggtggta tctggatccc ggcctccgat gttgatattc catacatgac
      601 cactcccttt tcattgacaa tatggaagcc gaaccttaca tcttctatat cctctttgaa
      661 aaatgctttg ataaatatgg aatacttctc ccccgattcc agcacagtga cgcgttcacc
      721 ttcggagtct agaatgccta tatccagcac ttcagcccct tcgcgagagc ccgctctcag
      781 caaccgtggg ttatttacag gtgaagacag ggggaccaat gactgttttt ctttggtttc
      841 gggtttccct ccatcagcag cgctttcagg agtcttgcca tcctggcgag ggttttgatc
      901 gacatacaaa attttccagt attttttagc aatcggatct ggcttaccga cttccaggat
      961 ctttcccttc tccagcaaaa tggccatatc gcaaatacca cgaatggaat tcatgtcatg
     1021 cgagacaaac aggatggtct ttccgacctt tcgaaattcc tcgattttgc caaacgattt
     1081 gcgctgaaaa cgagcatcac cgaccgaaag agcctcatca ataatgagga tatcagggtc
     1141 aatacaaacg gcggtggaga aggtcagccg ggcctgcata cctgaggaat acgttcggaa
     1201 aggctgatca atgaattccc ttaattcact aaattcaatg acccaatcct ctttctcctt
     1261 gatctcctcg ctggatagcc ctgcgatcaa gcctcccatg taaatatttt cccgcccact
     1321 gtattgggga ttaaaaccag tcccaagttc aagaattgag gaaattcgac cattgacctg
     1381 gatttcgccg gacgaggcat ccagggtgcc agcaatgatc tttaagagcg tactttttcc
     1441 ggctccatta cgaccgatca gccctacgac ctgccctcgt tggacatcga acgaaatatc
     1501 ctgcagggcc caaaatggtt tgtagagagg gcgtccgcta acgagttccc acaccatatc
     1561 agccgggcgg ttatagacct tgtatagctt ggaaagattt gccagatgaa tggcagagtc
     1621 tgctgagctg gtcataatgc atttccaaac atggttttca atctacggaa tacacgaaaa
     1681 ccaaaaatga agataaatac acttccaaag aggaagattg cccatgccca gggatgcgca
     1741 aaatgcccaa aatacagaat atcctggaag caccagatca tataactaat cggattaaga
     1801 tacaaaatag gccgcaccgc ggcagggatc gattcgggca aatagaggat gggtaagatg
     1861 aaaaatgcga tcgagagaaa aatttgtaca aagtctttaa gatctctgaa atacactcca
     1921 atggatgaaa ataagtaact gatccctatc atccccagcg tttgaaacaa aataagaacg
     1981 ggaaggaata catacgtcca cggaattgcg ccatttgtaa tcagcgtata aatggcaagc
     2041 aaaaacagaa aaatgacttc ggtcacaatg gtggccagca cactttttac cggcagcact
     2101 tcaagaggga aaatcacctg cttgacgata ttcgcgttcg cgatgatgac cgtgctggct
     2161 ttggccagcg cttcctgaaa catcagccag ggaatgatgc cagaaagaat ataagccgtg
     2221 taatccaatg gcatgtcgat ggtcccacct gtgcgagcac gaaagacaaa gccaaaaatg
     2281 aaaatgtaga caaaggtcag caacaggggg tgaccgattg cccaaagcac cccaaagacc
     2341 tgacccgtgt agcgttcacc gatctcccgt ttggtgagct cgtaaattaa gcggccccgg
     2401 cgcctgaata aagcgatcag ctcaaggaac gcctgaaaat tgctggaagg atttaagaca
     2461 gagtacataa cgtattcagg ctttctcttg atggatgatc ttgaaaccct gtccgtcggc
     2521 tttcaccacc gtgccgttcg gcgaagggct gagcgataat ccgttttcga tacaggtcag
     2581 gcgcataata tccataattt catcgctccc ctctaaaaag acggtcgtaa tttcgcgttg
     2641 tttgagctcc gaaacaattt cctttgcctt attgcggagc gaaccgtaga acttcagcga
     2701 atctctggca taggacatcg cccgttgtgt aaacaccgac atgccttccg gcgtcaggtc
     2761 gtattgcagg cgcgtccggt ccaaatgaga aaccttgacc cagccgcgat ggatgagccg
     2821 cttgatatac cagttcacac tgcccacagc gattcccagg cggtgtgata ggctcgcctg
     2881 ggtcacttta gaatcctggg cgatttcgtt caggaggtca tattcgtgtt gttttatatc
     2941 ttccataggg tgagcattca gtttctgaat tctcacatgg aactccccct ctgtcaagga
     3001 caggctagcg cgtcaggtta agcaaatact gcccgtagga gttaccggcg agcgactttc
     3061 ccaactcgcg caactgatcc aatgaaatat agcgcatgcg gtaggcgatt tcttccgggc
     3121 aagagatcat cagcccttgt cgttcctgga ttgcctcaat gaacgtggaa gctttaagca
     3181 aagactcatg tgtaccggca tccagccagg cgaccccgcg cccgaaaact tttgccttca
     3241 atgttccctg ctccaaataa atcttgttca ggtcggtaat ttccaactcg ccgcgggccg
     3301 atggtttgac agactcagcc gcctgcacaa ctttattgtc ataaaagtat attcccggga
     3361 cggcatattt cgagcgaggt gtagcgggct tctcctcaag gctaagaacg ttgtattgtt
     3421 cgtcaaattc tacaatgcca taccggtgcg ggtcattcac tttatacccg aaaatgatcg
     3481 cgccttcctt taacaaggca cactcctgta aaagggaagg gattccctgc ccataaaaaa
     3541 tattgtcgcc caggatcaaa catacagggc ttcccccaac aaactcgcgc cctaccagga
     3601 atgcatctgc cagaccgcgc ggctcgccct gctcagcata ttcaaacttc aaaccccatt
     3661 gagagccatc tttcaataaa tccctgaacg caggcaaggc atccggtgta ctaatgacaa
     3721 ggatttcacg aattcccgcc agcatgagca tggaaagcgg ataatagatc atcggcttgt
     3781 cgtacacagg caagatttgt ttgctgatgg caagcgtcag tgggtaaaga cgcgtggctt
     3841 ttccgcccgc caaaatgatc cccttcattt tgactcccgc tgtgcatcgg cgcgccatgc
     3901 ataattcttc tcgatccatt caccatacga gccgccgctt gcctgatcca cccattttgg
     3961 attcgacaaa taccattcaa tcgtcttacg gagacccctc tcaaatcgga cttcaggatg
     4021 ccagcccagc tcgcgcgtga tcctggctga atcgatcgcg tagcgacgat catggcccgg
     4081 gcggtctttt acaaattgaa ttaactctct gtaactcttt aagccgcgtt cattcaattg
     4141 cgggatttca accggtggcg cgatgtcatc cagaatgctg cagatctcat tgaccagatt
     4201 gatattcttt tgctcgttgt tggcaccaat cgcatactcg cctcccactt ttccgctttc
     4261 aaatgccagt tgaactgctt tgcagtgatc ttccacataa atccaatcgc ggacgttgga
     4321 tccgtcccca tagacaggca gggatttgcc agaaagtgcg ttcagaatca tgagcggaat
     4381 catcttctcg gggaattgat agggtccata attgttggag caattggtga tgatggtcgg
     4441 taagccatac gtatgatgat aagcccgaac gaaatgatca ctcgaagcct tcgaggctgc
     4501 gtatggggaa tttggtgcat agggcgtttc ttcagtgaaa taaccatcct ctccgaggga
     4561 accaaagact tcatccgtag aaacatgcag gaatcgaaag tcgcgtttca gatcttcagg
     4621 taacgtcgag tagtatgccc gcgccgcttc cagcaggcga taggttccaa ccacattcgt
     4681 ttgaataaac tcttcagggc tgacgattga acgatccaca tgcgactcag ccgccagatg
     4741 aaaaatgcct gcaggcttgt attgtgcgaa taatgactca acaagtttgc cgtcggtgat
     4801 atttcccttt acaaacacat aatcctgatt gccttcgaga tctgccaacg agagcgcatt
     4861 cgcagcgtat gtcagagcgt ccagattgac cacctgatag gttcgactgt ggatcaactg
     4921 ccttaccaaa tttggaccga tgaatccgtt cccgcccgtt acgatcactg ttttcttgtc
     4981 agccataatt cgccttctta tatttcaaat ttcgtctcaa ccagatcgct tttttgtcgg
     5041 atgatcccta aaaatatatt ttcatactga tcgacaattc ggtcaatctc gaaggacttc
     5101 gccttttcca gactggcatt gcggaatgat aacaggcgat ttggatcttc gagcaaattt
     5161 cgtaacaaca atgaaaaatg ggaaatagca ctccgctcca ccaaatatcc attttgagca
     5221 tcatccacca gatctacaaa tcctcctaca cggctggcaa caatcgccag ccctttggcg
     5281 agagcctgca cgcccacaac gggcagtcct tcggaaagag atggcatgaa caaaatatcg
     5341 ctacgatcaa accattgcat cacttcttgc ggggtaatcc agccggtgag gagaaagcga
     5401 tcttccaggt tagcttcgat aatccatttt tttaagtcag gaagcaaggt gccatctccg
     5461 ataattacac atctccaggg tatatccgtt atttccttaa gggactgcac taactgtaga
     5521 ggcgcctttt gcggcatcaa tcggccagca aaaacgatca ctggaggtgt attaacctga
     5581 attcgttcgg gccgcacggc cttaacgtca acaccgttgg gaataaccag gatttcctca
     5641 gggtagtttt tttgtgcaag ctcgcgcgta taagcactaa ccgccactcg gatc
//
LOCUS       DASTJP010000604         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_682000, whole genome shotgun sequence.
ACCESSION   DASTJP010000604 DASTJP010000000
VERSION     DASTJP010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682000"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFQ23_17800"
     CDS             <1..573
                     /locus_tag="VFQ23_17800"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET9908509.1"
                     /translation="PSVDVVTGLIKANFPARIAFAVASNTDSRVILDQPGAERLLGRG
                     DMLFQAPDAAAAVRLQGVFVSDHEIQNLVEFWRDQLGGASPYAAASQPVDLPKSDVPL
                     KQTPLFEDMSRADTATDPLLVEAIELVRKEGRASVSMLQRRMRIGYTRAARLVDVMED
                     RKIVGPPEGATQMRQILDYGQTAPPKDNGI"
     gene            648..719
                     /locus_tag="VFQ23_17805"
     tRNA            648..719
                     /locus_tag="VFQ23_17805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:681..683,aa:Arg,seq:cct)
     gene            complement(912..2174)
                     /locus_tag="VFQ23_17810"
     CDS             complement(912..2174)
                     /locus_tag="VFQ23_17810"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET9908510.1"
                     /translation="MIDSQAVASSGYFLSTERIVETLQAGLDGKFHNQRVLVLIPDHT
                     RSLPLPYLFRALVDILGHTRQLDFMVALGTHPPLDESDINKLVGVNSEERNTAFKHIR
                     LLNHAWNDPSALTAIGTLDQDEIKQLAGASWHESLPDRVEIRINKAALEYDHLIILGP
                     TFPHEVVGFSGGAKYLFPGISGADMIDATHWLGALAGVVNMIGFKDTPVRAMIHAAAR
                     KLSTPVTLIALVVEDHQLSGMFVGDYLSAWSSAADLSAVRHIRWVDKPYQRVLSCAPS
                     MYDELWTCAKAMYKLEQAVAEGGEVIIYAPHLEVVSQVHGKYIYEIGYHSLPYFLTQW
                     DRFKHIPLGVLAHSTHLRGSGNFENGIERANVKVTLASKISAEDCAQLNLGYLDPSEI
                     DLGEWQNREDEGVLFVARAGETLYRARQ"
     gene            complement(2171..3433)
                     /locus_tag="VFQ23_17815"
     CDS             complement(2171..3433)
                     /locus_tag="VFQ23_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HET9908511.1"
                     /translation="MSLDVQVIRQQFPSLERPAIFLDNPAGTQISKPSLDRINKYLLE
                     CNANHGGSFETSRKSDEILDEAHAAMADFLNAARPEEIIFGNNMTTLTLHVSRSLARN
                     LKEGDNILVTRLDHDANIAPWMLVAEDKGCNLIWVDIDVEAGTLDLDDFAKALERKPK
                     IAAFGYASNLLGTVNPVKELTKMAHEAGALVYIDAVQYAPHGPIDVQDIGCDFLACSA
                     YKFFGPHAGAVYGRYDLLDELKAYKVRPASDELPYKFETGTQNHEGIAGVLGALEYFE
                     WMGNQFGGDYAQAWKDAGFSGRRLVLKQAMSVMQEYETELSRALIQAIESVPGTRIHG
                     VTDLERLHERVPTVSFTLEGRRPEDIAVELGKQNIYVWNGHNYALAIVERMGLLEAGG
                     MIRVGPVHYNTLDEITKFREVLNNIVTR"
     gene            complement(3487..>3876)
                     /locus_tag="VFQ23_17820"
     CDS             complement(3487..>3876)
                     /locus_tag="VFQ23_17820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9908512.1"
                     /translation="ILAGLGFGGYFIFMHNSTQDTTTILWPMMTSRVAGTLLVLTFVL
                     ARREPLAVPRDAWGVVLINATLDLAGNFFYILASRAGRLDVAAVLSSLYPGATVVLAW
                     LLLKEHISRRQIVGILLALGAIALFTL"
BASE COUNT          973 a         1050 c          927 g          926 t
ORIGIN      
        1 ccatcggtgg atgtggtgac gggattgatt aaagccaact tcccggcgcg cattgcgttt
       61 gctgtagcat cgaacaccga cagccgcgtg atcctcgacc agcccggtgc ggaacgcctg
      121 cttggccgcg gtgacatgtt gttccaggcc ccagatgcgg ctgccgcggt gcgattgcag
      181 ggcgtgttcg tttcggatca tgaaattcaa aacctggtgg aattttggag agatcaactc
      241 ggcggagcaa gcccatacgc ggccgcgtca caaccggtgg atcttccaaa atcagatgtc
      301 cctctgaaac agacccctct gttcgaagac atgtcccgcg cggatacggc gaccgatccg
      361 ttactggtcg aggcgatcga actggtccgc aaggagggac gcgcctctgt ttccatgctg
      421 cagcgcagga tgcgcatcgg ctacacccgc gccgcgcgcc tcgtggatgt gatggaggat
      481 cgcaagatcg ttggtccacc ggagggagcc acgcaaatgc gtcagatcct cgactatggg
      541 caaaccgcac caccgaagga caatggtatc tagttggttg cacacgttcc atccgcagtt
      601 gaactgcgga tggaacctaa aaaatcaaat gatataattc ctcatgcgcc ctggtagttc
      661 aatggataga acaaggcact cctaaggctt agatgtgggt tcgattcccg cccggggcat
      721 agtcaaaaaa attcgtcgaa cgctttccaa ctgtaacctt ttcctccctt gtcaagaaaa
      781 taactttata gtaatatttg catcgacgaa cactagggtg cccccctcac cctggtgttc
      841 gtcatttata tccataaaca aaggaccagt ttttcaactg gtcctttgtt ttaatcccag
      901 atactgctga tttattgcct cgcgcggtat aacgtttctc ctgcacgcgc cacaaataga
      961 acaccttcat cttccctgtt ttgccactcg cccaggtcaa tctccgaagg atcgagatag
     1021 cccagattca actgcgcaca atcttccgcg gatattttgc tggcaagcgt gactttcacg
     1081 tttgcccttt caattccgtt ctcgaagtta cccgagccgc gtaggtgcgt actatgcgct
     1141 aaaacgccca acgggatgtg tttgaagcga tcccattgag ttagaaaata aggcagtgag
     1201 tgataaccaa tttcgtaaat atatttacca tgcacctggc tcacaacttc aagatgtggg
     1261 gcgtaaataa tgacttcacc accttctgcc acagcctgtt caagtttgta cattgctttt
     1321 gcacaagtcc ataattcatc gtacatagat ggcgcacagg agaggacgcg ttgataaggc
     1381 ttgtcgaccc aacggatgtg gcgtactgcg gaaagatctg cagcactgct ccaggctgat
     1441 aaataatccc caacaaacat accgctaagt tggtgatctt ccacaaccag cgcgatcaaa
     1501 gtaacgggcg tggataattt cctggctgcg gcgtgaatca ttgcgcgcac cggagtatct
     1561 ttgaatccaa tcatattcac gacacctgcc agtgccccaa gccaatgcgt ggcatcgatc
     1621 atatctgccc ctgaaatgcc ggggaataga tatttcgcgc cgcccgaaaa acccaccact
     1681 tcatgtggga aggtgggacc aagaatgatg agatggtcat actccagcgc ggctttgttg
     1741 atgcggatct caactctatc gggcaacgac tcatgccacg aggcacccgc cagctgtttg
     1801 atttcatcct ggtccagcgt gccaatggcg gttagtgctg acggatcatt ccaggcatga
     1861 ttgagcaggc ggatgtgttt aaaagctgta tttcgttctt ctgaattcac tccaaccagt
     1921 ttattgatat cggattcgtc caaaggagga tgagtaccca gcgccaccat aaaatccagt
     1981 tgccgggtgt gaccaagaat atccacaagc gcgcggaata aatatggcaa tggcagtgaa
     2041 cgggtatgat ctggaattag aaccagcacg cgttggttgt gaaatttacc atcaagacca
     2101 gcctgtaacg tctcaacgat acgctcagtc gagagaaagt atccacttga cgcaaccgcc
     2161 tgcgaatcga tcatcgtgta acaatattat ttaatacttc tctgaacttt gttatctcat
     2221 ctagcgtgtt gtaatggacg ggtcccacac ggatcattcc gcccgcttcc agtaaaccca
     2281 tccgttcaac aatagccaaa gcatagttat gtccattcca aacataaatg ttttgctttc
     2341 cgagttccac cgcaatgtcc tcgggacgcc tgccctcaag agtgaacgaa acagtcggaa
     2401 cgcgttcatg caaacgctcc aaatctgtca caccatggat tcgagttccc ggaacggatt
     2461 cgatagcctg aatcaatgct cgggataact cggtctcgta ttcctgcatg acagacatgg
     2521 cctgcttgag gactagtctg cgaccagaga agcccgcatc tttccatgct tgtgcgtagt
     2581 cgccgccaaa ctgattcccc atccactcga agtattccag cgctcctaac acgccggcga
     2641 tcccttcgtg attttgtgtc cctgtttcaa atttatacgg cagttcatcg gaagctggcc
     2701 gcaccttata tgccttcaat tcatcgagca gatcatatct tccatatacg gcgccggcat
     2761 gcggaccaaa gaatttataa gcggagcagg ccaaaaaatc gcagccaata tcctgcacat
     2821 cgatcggacc gtgcggcgca tactgaaccg catcaatgta caccaatgcg ccagcctcgt
     2881 gtgccatctt tgttaattcc tttacagggt taaccgttcc cagcagattc gatgcgtagc
     2941 cgaacgctgc gatctttggc ttacgttcca gtgctttcgc aaaatcatcg agatcgagcg
     3001 tgccagcttc tacgtcgata tcgacccaga tcaaattaca gcccttatcc tccgccacaa
     3061 gcatccaggg cgcaatattt gcatcgtggt caagccgcgt cacaaggatg ttatctccct
     3121 ctttcaaatt tctcgccagc gaacgagaga cgtgcagcgt cagcgtggtc atattgttgc
     3181 caaagataat ttcttccggg cgcgcggcat tgagaaaatc tgccatggcg gcgtgagctt
     3241 cgtccaggat ctcatccgat ttgcgactgg tctcaaagct gcccccgtga ttcgcattac
     3301 attcaagtaa atatttattg atacgatcca ggctgggctt tgagatctgt gtaccggctg
     3361 ggttatcgag gaaaattgca ggtcgttcca gtgatgggaa ttgctggcgg atgacttgta
     3421 catcaagcga catggttagc tcctatagtg ttatcaattc tactcttcat caagggaaac
     3481 ttgatcttag agcgtaaata aagcgatcgc gcctaaagcc agcaaaatgc caacgatttg
     3541 ccggcgcgaa atgtgttctt tgagcagcag ccaggcaagg acaactgtcg ctccgggata
     3601 taacgaactc aggactgctg caacatccag ccgcccggct cggctggcga ggatatagaa
     3661 gaaattacct gccagatcca acgtagcatt gatcaagaca actccccaag cgtctcgcgg
     3721 gacagccaat ggctcacggc gcgccaacac aaaggtcagc accagcaacg ttcccgccac
     3781 gcgtgaggtc atcatcggcc agagtatggt cgtcgtatcc tgcgtcgaat tatgcataaa
     3841 gataaagtag ccgccaaaac ccagccccgc cagaat
//
LOCUS       DASTJP010000605         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_578596, whole genome shotgun sequence.
ACCESSION   DASTJP010000605 DASTJP010000000
VERSION     DASTJP010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578596"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFQ23_17825"
     CDS             <1..509
                     /locus_tag="VFQ23_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908513.1"
                     /translation="EMPEDVRRNYENAMRQLGDSDRDGLPDLLENLTNPTDQNKNGMP
                     DAIENMVSNVFSSTTRVIVNGAEYNSLDELPPEVRAQYEQAMGSFDANKNGVPDFLEG
                     FLNTTNPASIAQQSQVAAPSHVSKPISSSPTIEPESTNGWMIALLAIALFGVCAAGAV
                     GIWYFFLR"
     gene            676..1611
                     /locus_tag="VFQ23_17830"
     CDS             676..1611
                     /locus_tag="VFQ23_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9908514.1"
                     /translation="MIQVDGLIKDYGARRALDNITFDANQGEIVGFLGPNGAGKTTTM
                     RILTGYMPPTDGTAIVAGYDVVEESLEVRKRVGYLPETVPLYTDMTAVDYLKFMADLR
                     HIPDSEERAYETLETVGLQDRATGFIGNFSKGMRQRIGLAQALIHRPEVIILDEPTIG
                     LDPAQVVEIRNVIRDIGKDRTVLLSTHILSEAQQLCDRVLIINKGQIVAEDTPENLQS
                     RLVGSQRVVLRVRGDSDELPAKVNKVKGVRDVEAKPDGSVEFEFSAGQDVRPQVAKAV
                     IQAGFDLLEMRPIGMSLEEIFLELTRESAAGKKSS"
     gene            1621..2367
                     /locus_tag="VFQ23_17835"
     CDS             1621..2367
                     /locus_tag="VFQ23_17835"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908515.1"
                     /translation="MRNIWTIAKREYNNYFISPLAYVVAFSILMPIGIYFAIIVFATS
                     QQAFSGAPPPDITPITWLFCFLLVFTTPVITMRLLSDEARMGTLELLLTAPIRDFELV
                     FGKWLGAFLFILSILAITLVFPIILHFALVSPGIDQKLFLSAYLGVILVAAAFLALGV
                     GISAIFTNQFAAFFATFGLFFFLWFMVSLPASLIPSGGDVLNYLNMSTHFNEAFNAGN
                     IRLSDIVYYISLIALGLFMGTTAIEVRRWR"
     gene            2367..>3527
                     /locus_tag="VFQ23_17840"
     CDS             2367..>3527
                     /locus_tag="VFQ23_17840"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HET9908516.1"
                     /translation="MKNETKPTSTRNYSIAALIVALVACIATFFLGVVRGLVAAQVFT
                     VTNVENLQRYLIVSAGFIILGLAAYAILEPERVRRFLTGRQARYGSNAVIMSIAFAGI
                     LIIGNVLAFQNPLPLGDLTEDQVNTLAPETLAAIKQLPQKVTAIAFFSQTSNRETATQ
                     LLDKIKSNSNGQFDYEFIDPDRDPQTALNAGITGDGKILLQMGELKEIVAFASETEIL
                     KGMLRLLNPGTDAIYFTTGHGEQDIEQGSETSLTRAKATLESKNYAVKTLNLLVENAI
                     PEDASVIVIAGPLQPFTQDEVDLLKAYLANGGSLVVMSNPTGLTEFGDQDDPIADMLA
                     TDWGILLNNDIVIDLNSPQPTTATAAFYDSHPVTTNMNNLVSFYPFTRSLSAT"
BASE COUNT          877 a          879 c          845 g          926 t
ORIGIN      
        1 atgagatgcc cgaggatgtt cgtagaaatt atgaaaacgc gatgcgccag cttggcgaca
       61 gcgacaggga tggccttccc gacctgcttg aaaatttgac gaacccaacg gatcagaata
      121 aaaacgggat gcctgatgcg attgaaaaca tggtatccaa tgttttttcg tcaaccacca
      181 gagtcattgt caacggagcg gaatacaata gtttggacga gcttccgcct gaggtccgcg
      241 cgcaatacga acaggctatg ggatcctttg atgcaaacaa gaacggggtt ccagatttcc
      301 tggaagggtt tctcaataca acaaacccgg caagcatagc gcaacaaagt caggttgctg
      361 ctccctcgca cgtttccaaa ccgatctctt cgagcccaac cattgaacct gaatctacaa
      421 atggatggat gatcgctctg ttagcgatcg ctctattcgg tgtgtgtgcg gcgggtgcgg
      481 tcggcatttg gtactttttc ttaaggtgat acaattcgct ttattattag cgtttgatta
      541 gaaattcaca agtcacttga cttcgatttt gaatttctgt atattatttt cgatatctat
      601 ttcggcacgg cggcccaccg gcaggcgtgc cttttctttg tttccagata tttattccac
      661 ttctaaggag caagaatgat tcaggtcgat ggtctaatca aggactatgg cgcgcggcgc
      721 gccctcgata acatcacctt cgatgccaat cagggagaaa ttgttggctt tcttgggccg
      781 aacggtgcag gcaaaacaac aacgatgcga attctcactg gctatatgcc gccaaccgat
      841 ggaacagcca tcgtagctgg ttatgatgtc gtcgaggaat cactggaggt tcgaaaacga
      901 gttgggtatc ttccagaaac ggtgcccctt tacactgaca tgacggcagt agattacctt
      961 aagttcatgg ccgacttacg tcacatcccc gattcagaag agcgggccta tgaaacgctg
     1021 gaaacagtcg gcttgcagga tcgcgccacc ggatttattg ggaacttttc aaaaggcatg
     1081 cgccagcgga ttgggcttgc tcaggcgttg attcatcgcc ctgaagtaat tattctcgac
     1141 gaacctacca ttggtcttga ccctgcacag gtcgtcgaaa tccgcaatgt gatccgcgac
     1201 attgggaaag atcgaaccgt attgctttcc acacatattc tttcggaagc acaacagtta
     1261 tgtgatcgag tcctgattat caataaagga caaattgtag cggaggacac acctgaaaac
     1321 cttcagtcca ggctggttgg ttcacaacgc gttgtcctac gcgtccgcgg cgactcagat
     1381 gagctaccgg caaaggtcaa taaggtaaaa ggcgttcgag atgtggaagc aaaaccggat
     1441 gggtcggtcg aatttgaatt ttcagccggg caggatgtgc gcccgcaggt agcaaaagct
     1501 gtaattcaag cggggtttga tctgcttgaa atgcgtccga tcgggatgag cctcgaggaa
     1561 atcttccttg agcttactcg cgaaagcgct gctggcaaga aatcttcata gaggtaatcc
     1621 atgcgtaaca tttggacaat agcaaagcga gaatacaaca actactttat cagccccctc
     1681 gcgtatgtgg tggcattttc cattctcatg cccattggca tttattttgc catcattgta
     1741 tttgccacat cgcaacaggc gttctctggt gctccccctc cggatatcac tccgatcacc
     1801 tggctgtttt gtttcctgct ggtattcacc acacctgtaa taaccatgag gcttctctcc
     1861 gatgaagctc gaatgggaac cctggaattg ttactgacag ctccgattcg cgactttgag
     1921 cttgtctttg gcaaatggtt aggtgcattt cttttcatac tgtctattct ggcgatcaca
     1981 ttggtctttc caattatttt gcacttcgcc ttggtgagtc ccggcatcga tcaaaagctt
     2041 ttcctgtcag cctatctggg tgtgattctg gtggcagctg ccttcctggc cttgggcgta
     2101 gggatatcag cgatcttcac caaccaattt gctgccttct tcgcgacctt tggcttgttt
     2161 ttctttctat ggttcatggt gagcctgcca gccagcctaa tccccagtgg cggagatgtg
     2221 ctcaattacc ttaatatgtc aacacacttc aatgaagcct tcaatgcagg caatataaga
     2281 ttatcggaca ttgtttacta tatcagcctg attgcgcttg gattgttcat gggcaccact
     2341 gccattgaag ttcggagatg gcgctaatga aaaacgaaac aaaaccaacc tccacacgaa
     2401 actattccat tgcagcacta attgttgccc tggttgcctg tatcgctact ttcttcctgg
     2461 gcgtggtacg cggtctggtt gctgcccagg ttttcaccgt tacaaatgta gaaaaccttc
     2521 agcgctatct gatcgtgagt gccggattta tcatccttgg actcgcggcc tatgcaatcc
     2581 tggagcctga acgcgtccgc cgctttttga ccggtcgaca ggcgcgctat ggaagcaatg
     2641 ctgtgatcat gtcaattgct ttcgcaggca ttcttatcat tggcaacgtg cttgcgtttc
     2701 aaaatcccct cccgctcggc gacctgacgg aagaccaggt taacaccctt gcgcccgaaa
     2761 ctctcgctgc aattaaacag cttccgcaaa aagtcacagc gattgcgttc ttctcgcaaa
     2821 ccagcaaccg ggagactgcg acccagctat tggataagat caagtcgaat agcaatggac
     2881 aatttgatta tgaatttatc gaccctgatc gagatccaca aacggcgctc aatgcgggca
     2941 tcaccggcga tggaaagatc cttttgcaaa tgggtgaatt aaaggaaatc gtcgctttcg
     3001 cgagtgagac agaaatcctt aagggaatgc tgcggcttct aaacccgggc accgatgcca
     3061 tttatttcac aacaggtcat ggcgagcagg atatcgaaca gggtagtgag acttcactga
     3121 cgcgagcaaa agccaccctt gaaagcaaga actatgcggt caagacctta aatcttctgg
     3181 tagagaatgc aatccctgag gatgcaagtg tcattgtgat tgcgggacct cttcagccat
     3241 ttacgcaaga cgaagttgat ttgctcaaag catatcttgc caatggtggc tccctggttg
     3301 tgatgtccaa cccaacgggc ctgacggaat ttggtgatca ggatgacccg atcgctgaca
     3361 tgcttgccac cgactgggga attctcctca acaacgacat cgtcattgac ttgaatagcc
     3421 ctcaacctac gacagccaca gccgcgtttt atgattcgca tccggtcacg accaatatga
     3481 acaatctcgt ttccttttat ccatttacga gaagcctgag tgcaacc
//
LOCUS       DASTJP010000606         7624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1261886, whole genome shotgun sequence.
ACCESSION   DASTJP010000606 DASTJP010000000
VERSION     DASTJP010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261886"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1797
                     /gene="ilvD"
                     /locus_tag="VFQ23_17845"
     CDS             127..1797
                     /gene="ilvD"
                     /locus_tag="VFQ23_17845"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9908517.1"
                     /translation="MSDLKKNSHAITTGPNRAPARSMLKAVGFTDDDLRKPIVGVAST
                     WIEIGPCNYHLRRLSQKVKEGIRAAGGTPMEFNTVSISDGITMGAEGMKASLISREVI
                     ADSIELVARGNHFDAIVCLSGCDKTNPGVMMALARLDIPGLALYGGSINPGKLGEKSL
                     TIQDVFEAVGAYSAGKISEQEFMDVENLACPGAGACGGQFTANTMSTIMEFIGISPVG
                     FNSIPAMSEEKDTVAFEVGKVVMDMLRADLRPSQIYTRKAFENAITSVAATGGSTNAV
                     LHLLALAREANVDLHIDDFDAICSRVPLITDIKPFGKYMAADMHAAGGIPFLMKRLLE
                     GGYLHGDAITVTGKTISEAAQTGVETPGQQVIYPLEKPIKSNGGLVILHGNLSEEGGV
                     HKAAANNVKQFTGPARVFEREEDAMHAVTEQKIQSGDVVVIRYEGPKGGPGMREMLGV
                     TAAIAGAGLSDSVALLTDGRFSGATRGISVGHIAPEAYVGGMIAYVNDGDVITIDVDK
                     RELRVDIPEKELAQRKDLWKAPEARYKKGVMAKYARSVSSASEGAITN"
     gene            1873..3564
                     /gene="ilvB"
                     /locus_tag="VFQ23_17850"
     CDS             1873..3564
                     /gene="ilvB"
                     /locus_tag="VFQ23_17850"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET9908518.1"
                     /translation="MKMKGAEIVWECLTREGVEVVFGYPGGANMPIYDAMLNYPVHHV
                     LVRHEQGAAHMADGYARASGKVGVAMATSGPGATNLVTGICTAMMDSVPTVFITGQVA
                     AHLIGGDAFQETDVTGITLPITKHNYLVTRADQIAETIREAFYIARSGRPGPVLIDIC
                     KNAQVESTEFIYPEDVKLLGYQPVTRSPRNLLDQAVELIEKAKRPIILCGHGVIMSGA
                     EEELMQIAVKTQTPVASTLLGLGAFPASHPLSLGMMGMHGEAYTNLAIQNSDLILAFG
                     MRFDDRVTGTLKTYAPKAKKIHIEIDPSEIHKNVFVDVPLVGDLKTVLRELIPLVDEY
                     DHEEWKQEIDAWKEEVDSRSIMNWEEDGKLHVSHLIDDIWKATGGGAIVTTDVGQHQM
                     WAAQYYFLEKPNRWITSGGAGTMGFGLPSAIGAWFAAKDQEIWTVVGDGGFQMTAAEL
                     TTAIQEGANVKVAIMNNQYLGMVRQWQEFFFEKRYSAVNMLTPDFVKLADAHGVPARR
                     VTRREEVADAIEWARNTPGTVVIEFRVEQEEAVFPMVPQGAALHEMIRRPVKQKA"
     gene            3646..4206
                     /gene="ilvN"
                     /locus_tag="VFQ23_17855"
     CDS             3646..4206
                     /gene="ilvN"
                     /locus_tag="VFQ23_17855"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET9908519.1"
                     /translation="MNYTFIALVENKPGVLNRVASLFRRRNFNIESLAVGRSDNPEVS
                     RMTIVVDCPNGDVDAHRIEANLYKLVNVIDVQDVTHQPSVTRDLALIKVQVGPDRRAE
                     VNGLAEIFRARIVDVAPDSVIVEITGTEDKIEGMIELLRPIGIVEMVRTGQVSMTRGV
                     NDGIRRVAGTNGNRYEDVMELAEMAP"
     gene            4352..5404
                     /gene="ilvC"
                     /locus_tag="VFQ23_17860"
     CDS             4352..5404
                     /gene="ilvC"
                     /locus_tag="VFQ23_17860"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET9908520.1"
                     /translation="MTRINFGGVEEEVVTRDEFPLEKAREVLKDEVVAVLGYGVQGPA
                     QALNMRDNGINVIVGQREGTKNWDKALADGWVPGETLFSIEEAAERGTIVQYLLSDAG
                     QRSQWSKIVECLDEGDMLYFSHGFSVTFKDDTGVIPPPHVDVALVAPKGAGRSVRQNY
                     LDGSGINASFAVHQDATGRAYERTVALGIAIGAGYLFPTTFQNEVYSDLTGERGVLIG
                     ALSGLMEAQYNVLRKHGHSPSEAFNETVEELTQSLIRLVGQNGMDFMYANCSTTAQRG
                     ALDWHARFRDAVAPVFDDLYASVTSGNEARITLDANSRVDYREQLDKELAAIRNSEMW
                     QAGAAVRSLRPENWKQ"
     gene            5569..7215
                     /locus_tag="VFQ23_17865"
     CDS             5569..7215
                     /locus_tag="VFQ23_17865"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET9908521.1"
                     /translation="MTNNYVRIFDTTLRDGEQSPGATMTSAEKLEVARSLARMGVDII
                     EAGFPAASPDDLEAVRRIAVEIGKMDPSDPEARVPIIAGLARANKADIDKVWEAVQEA
                     DKPRIHTFLATSPIHMQHKLKMDPEEVIQRVSEMVAYARSMCEDVEFSPEDAGRSDPE
                     FLYVVLGEAIKAGATTLNIPDTVGYTTPDEFYNLIDGIIKNTPGMHAGITVSVHCHDD
                     LGLATANTLAGIRAGARQAEVTINGIGERAGNTSLEEVVMALKTRHPVFELETGIDTK
                     QISRISKLVSNYTGIVVQPNKAIVGSNAFAHEAGIHQDGMLKHNLTYEIMRPEDVGVN
                     QTKLVLGKHSGRHALRNRLAEMGHSLDEFELDKAFARFKELADRKKVITDADLEALIA
                     DEFYRPRDVYFLDGMQVTCGTMGMPTATVRLKGPDGKVQVHASMGSGPVDAAYKAIDS
                     IVKTPNTLLEFNIHAITEGIDAIGEVTVRVQPKDEGITTMDAQSEIPYARVFGGHGAD
                     TDIIVASAKAYLNAINKLIIAQTEGLNPVHANHNHGVMLG"
     gene            7391..>7624
                     /locus_tag="VFQ23_17870"
     CDS             7391..>7624
                     /locus_tag="VFQ23_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9908522.1"
                     /translation="MPKTLFEKIWDSHVVTEQPDTPAVLYIDLHLVHEVTSPQAFTGL
                     RQRGLKVRRPEKTLATMDHSIPTTPLNVPIADAM"
BASE COUNT         1874 a         1867 c         2190 g         1693 t
ORIGIN      
        1 aagaaataac acacacctga gtaaaagcaa ggagccgccc taaccacggg cggttctttt
       61 attacccaat cccaccacga cctctcccca tgacaaagtc atggagagga gacctatgag
      121 gagcttatgt ctgatctcaa aaagaacagc catgccatta ccacaggacc caaccgagcg
      181 ccggcacgct ccatgctcaa agcggtgggt ttcactgatg atgacttgcg taaacccatt
      241 gtaggtgtcg ccagcacgtg gattgagatc ggtccgtgca attatcacct gcgaaggtta
      301 tcgcaaaagg tgaaggaagg catccgggcg gcaggcggaa ctccgatgga gttcaatacg
      361 gtgtccattt cagatggcat caccatgggt gcggagggca tgaaagcgtc gctcatcagc
      421 cgagaggtaa ttgctgattc aattgaactg gtggcacggg ggaatcattt cgatgctatt
      481 gtttgtcttt caggttgcga caagaccaat cccggtgtga tgatggcact agcccggctc
      541 gatatccctg gcttggcgtt atacggcggc tcgatcaacc cgggcaaact cggtgaaaag
      601 tcactgacaa tccaggatgt gtttgaagca gtgggggcct actcagcggg gaagatcagt
      661 gaacaggaat ttatggatgt tgaaaactta gcgtgtccgg gtgctggtgc ctgtggcgga
      721 cagtttaccg ccaacaccat gtccaccatc atggagttta tcggtatctc gccagtggga
      781 ttcaacagca tcccggcgat gtctgaagag aaagacacag ttgcttttga agtggggaaa
      841 gtggtcatgg atatgttgcg tgcggattta cgcccctctc aaatttacac acgaaaagct
      901 tttgaaaatg cgatcacctc cgtcgctgcg accggtggct cgacgaatgc ggttctccat
      961 cttttggcat tggcgcggga agcgaatgtc gatttacaca tcgatgattt cgacgccatc
     1021 tgctcgcgcg ttcctttgat cacagacatc aagccgttcg gcaaatatat ggctgctgat
     1081 atgcacgccg caggcggcat cccatttttg atgaagcgtt tgttggaagg tggctatttg
     1141 cacggggatg cgatcacggt cactggcaag actattagcg aagctgctca gacaggcgtc
     1201 gaaacgccgg gtcaacaagt gatctatccg ctggagaaac cgatcaaatc caacggtggt
     1261 ctcgttatct tgcatggcaa tttgtctgaa gaaggcggtg tacataaggc tgcggcaaat
     1321 aacgtaaagc aattcacggg tccggcgcgc gtgttcgagc gtgaagagga tgctatgcac
     1381 gcagtcacag agcaaaagat ccaatccggg gatgtggttg tcatccgtta tgaggggccg
     1441 aagggcggac ctgggatgcg cgaaatgctt ggggtcacgg cggcgatcgc gggcgcggga
     1501 ctgagtgaca gcgtcgcctt attgaccgat ggaaggtttt ccggtgcgac gcgcggcatt
     1561 tcggttgggc atatcgcgcc tgaggcctat gtcggtggga tgatcgctta cgtcaatgat
     1621 ggtgacgtga tcacgatcga tgtggataaa cgcgagttgc gcgtggatat ccccgaaaag
     1681 gaattggcac aacggaaaga tctttggaag gcacccgagg cacgctacaa aaaaggtgtg
     1741 atggcaaagt atgccagaag tgtttcctcg gcttctgaag gcgcgattac aaattaatcc
     1801 tgccgccgag acgcggagat acagagagaa aagaatctct gcgctctctg tggcagaaaa
     1861 taaggagcaa ttatgaaaat gaagggcgct gaaattgttt gggaatgttt gacccgtgaa
     1921 ggggtggaag tagtgtttgg ctatccgggt ggcgcgaata tgccgattta cgacgccatg
     1981 ttgaactatc cggtgcatca tgtgctggtt cggcacgagc agggcgcggc gcacatggca
     2041 gatggatacg cgcgtgcctc ggggaaagtt ggcgtggcaa tggcgacctc cggtcccggt
     2101 gcgacgaatt tggtcactgg gatttgtact gcgatgatgg attcggttcc gactgtgttc
     2161 atcaccggtc aggtcgcggc ccatttgatc ggtggtgacg ccttccagga aacagatgtg
     2221 acaggtatca cactgccgat caccaaacac aattaccttg tgacgcgtgc agatcaaatt
     2281 gcagaaacga tccgggaagc gttttacatc gcccgcagtg gaagacccgg cccggtattg
     2341 atcgatatct gtaagaacgc tcaggtggag tccaccgagt ttatttatcc cgaagacgtg
     2401 aaactgctag gctatcagcc cgtgacgcgc tcgccgcgca atcttctgga ccaggctgtt
     2461 gagttaatcg agaaggcaaa gcgaccgatc atcctttgtg gtcatggtgt gatcatgtct
     2521 ggagccgaag aggagttgat gcagatcgcg gtcaagacgc aaacgccggt tgcaagcacg
     2581 ctcttaggac tcggcgcgtt cccggcctca catcccctga gccttggcat gatgggcatg
     2641 catggggaag cctataccaa cctggcgatt caaaattcag atttgatcct tgccttcggc
     2701 atgcgctttg atgatcgcgt gaccggcacc ttgaagacgt acgctccgaa ggcgaagaag
     2761 atccatattg aaatcgaccc ctccgagatt cataagaatg tttttgtgga tgttccattg
     2821 gtgggcgatt tgaagacggt gctccgtgaa ctgataccgt tagtcgatga gtacgaccac
     2881 gaggaatgga aacaggaaat cgatgcctgg aaagaggaag tcgattcacg cagtattatg
     2941 aattgggagg aagatggcaa gctccatgtg tcacacctga ttgatgatat ctggaaggca
     3001 actggcggcg gtgcgatagt cacaacagat gtcggacagc atcagatgtg ggccgcgcaa
     3061 tattattttc tggaaaagcc gaatcgctgg attacctccg gcggcgcagg gacaatgggt
     3121 tttggccttc cctcggccat cggtgcctgg tttgcagcga aagaccagga aatctggaca
     3181 gttgtgggtg atggcggctt ccagatgacg gcggccgagt tgaccactgc cattcaagaa
     3241 ggtgcgaatg taaaagtggc catcatgaac aatcaatatc tcggcatggt tcgccagtgg
     3301 caggagttct tcttcgagaa gcgttactcg gcggtcaaca tgctcacgcc tgattttgta
     3361 aagctcgctg acgcacatgg cgtcccggca agacgcgtca cccgacgcga agaagttgca
     3421 gatgccattg aatgggcgcg caacactccc ggcactgttg ttattgagtt ccgcgtggag
     3481 caggaggaag cggtcttccc aatggtccca cagggtgccg ctttgcatga aatgatacgc
     3541 cgccctgtga agcagaaagc atagtccgca gattgcgctg atttcgcaga ttgaaataat
     3601 aatctgtgca atctgcaaaa tcagcggata agaaatggag acagaatgaa ttacaccttt
     3661 atcgcattag tggaaaacaa acctggtgtc ctcaatcgag tggcgtcact gtttcgccgc
     3721 cgcaatttca acatcgagtc tcttgcggtg gggcggtcag ataacccgga agtctcgcgt
     3781 atgacgatcg tggtggattg ccccaacggt gacgtggacg cgcatcggat cgaagcgaat
     3841 ctctataaac tggttaatgt cattgacgtg caggatgtca cacatcaacc gtcggtgacc
     3901 cgcgacctgg cactgatcaa ggtgcaggtc ggacctgacc gccgcgccga ggtcaacgga
     3961 ctcgctgaaa tcttccgcgc ccgcatcgtg gatgtggctc ccgactcggt cattgtggaa
     4021 atcactggaa cggaagacaa aattgagggc atgattgaac tcttgcgccc gatcggcatt
     4081 gtcgaaatgg ttcggaccgg acaggtatcc atgacacgtg gcgtcaacga tggtatccgt
     4141 cgtgtggctg ggacgaatgg caatcgctat gaggatgtga tggaactagc ggagatggcg
     4201 ccgtaattag agcctgtcat tgcgaggagc gaagcgacga agcaatctca tcgcacctgc
     4261 aatacagacg tttgttgata attatgagat tgcttcgggc ggaccatcac cgccctcgca
     4321 atgacagatg gaatacagga gaagagctct tatgaccaga attaatttcg gtggtgtgga
     4381 agaagaagtt gtgacgcgcg acgaattccc gctcgaaaaa gcgcgggagg tcttaaagga
     4441 tgaagttgtt gccgtgcttg gttatggcgt gcagggacct gcccaggcat tgaacatgcg
     4501 cgataacgga atcaatgtca ttgtgggtca gcgggagggc acaaagaatt gggataaagc
     4561 cctggcagat ggctgggttc caggcgagac gttgttctcg attgaagaag ctgccgaacg
     4621 cggaacgatc gttcaatatc ttttatccga tgccggtcag cgttcgcagt ggtcgaagat
     4681 cgtggaatgt cttgatgaag gggatatgct ttatttctct cacgggtttt ctgtcacgtt
     4741 caaagatgat acgggtgtca ttccacctcc gcatgtggat gttgcactcg ttgccccgaa
     4801 gggtgcaggg cgtagtgtcc gtcagaatta tctggatggt tccgggatca atgcgagttt
     4861 cgcggttcat caggatgcga ctggccgagc ctacgagcgc acagttgcct tggggattgc
     4921 gattggtgcc ggctatcttt tcccgacgac tttccaaaac gaagtttata gtgatctcac
     4981 cggtgagcgc ggcgtattga tcggcgcgct ctcggggctc atggaagcgc aatacaacgt
     5041 tcttcgcaag catggtcata gtcccagcga agctttcaac gagacagttg aagaactcac
     5101 gcagtcgttg atccgcctgg tcggtcagaa tggcatggac tttatgtatg ccaactgttc
     5161 cacgaccgcc caacgcggtg cgctggattg gcatgcccgc tttcgcgatg cagttgcgcc
     5221 ggtctttgat gacctctatg catccgtgac cagcggcaat gaagcacgta ttacgttgga
     5281 cgccaacagc cgcgtcgatt atcgcgagca attggataaa gaactcgctg ccattcgtaa
     5341 ctctgaaatg tggcaggctg gtgcagcagt gagatcgttg agacctgaga attggaaaca
     5401 gtaataatat cgatggtcga gtgcgccgca ggcgtgtatc gagaccgcca catttcaaga
     5461 aatatttaga cgaaacacgt tcgtctcgac acctgcactt gcgcgctggt gcaagtgtac
     5521 gccccaacga tcactgggct actagaccaa cgccgaaagg aagacactat gacaaacaac
     5581 tacgttcgta tctttgacac aacactacgg gatggggaac aatcccctgg cgcgacgatg
     5641 acctcggccg aaaagctgga agtggcgcgg tcactggcgc gcatgggtgt ggatattatc
     5701 gaggctggct ttcctgcggc gtcaccggat gatctggagg ctgttcgacg gatcgcggtc
     5761 gaaatcggta aaatggaccc aagcgatcca gaggcgagag tacccatcat cgccggactg
     5821 gcgcgggcca ataaggcaga tatcgataaa gtgtgggaag ccgtacagga ggccgataaa
     5881 ccgcgcatcc acaccttcct tgcaacctcc ccgattcaca tgcagcacaa actcaagatg
     5941 gatcctgaag aagtgatcca gcgcgtttct gagatggttg cttatgcacg atccatgtgt
     6001 gaagacgttg agttttctcc tgaggacgcg gggcgctctg accctgagtt cctgtatgtc
     6061 gttttgggtg aagcaatcaa ggcgggagcg acgaccttga atatccctga taccgttggg
     6121 tacactacgc cagatgaatt ctacaattta attgatggca ttatcaagaa cactccgggt
     6181 atgcatgctg gcatcactgt ctccgtgcac tgtcatgatg atcttggtct ggcaacagcc
     6241 aacacactgg cgggcatccg cgccggcgca cgtcaggcgg aagttaccat caatgggatt
     6301 ggtgagcgtg ccggaaacac atcgctggaa gaagttgtga tggcactcaa gacccgtcac
     6361 ccggtctttg aactggaaac ggggattgat acaaaacaga tttcgcgcat cagcaaactg
     6421 gtcagcaatt acacaggcat cgttgtgcaa cccaacaaag ccattgttgg gtctaatgct
     6481 ttcgcacatg aggccggcat tcaccaggat ggaatgctca agcacaatct cacctatgag
     6541 atcatgcgcc ccgaagatgt gggcgtgaat cagaccaaac ttgtgctggg caagcattcg
     6601 gggcgtcatg cgttgcgaaa ccgcctggct gaaatgggac attccctcga tgaattcgaa
     6661 ctcgacaaag ccttcgcgcg cttcaaggaa ttggcagacc gcaagaaagt catcaccgat
     6721 gcggatctgg aagccttgat cgccgatgaa ttctatcgcc cgcgcgacgt gtacttcctg
     6781 gacgggatgc aggtcacctg cggaaccatg ggcatgccta ccgcaaccgt ccgcctgaag
     6841 ggaccggatg gcaaagttca ggtacatgcc tccatggggt ctggcccggt ggacgcggcg
     6901 tacaaggcga ttgactccat tgtcaaaacg ccaaacacgc tgcttgaatt caacattcat
     6961 gcgatcaccg aggggattga cgcgattggc gaagtcactg tccgcgtgca gccaaaggac
     7021 gagggcatca ccacgatgga cgcgcagagc gagattccct atgcgcgtgt gttcggcggg
     7081 cacggtgcgg ataccgatat catcgttgcc agcgcgaagg cctatctgaa cgccatcaac
     7141 aaattgatca tcgcacagac ggaaggattg aatcctgtcc atgccaatca taaccatggc
     7201 gtgatgttag gataaaaagc taattcggtg gtcgagtagg gcgacacttg cgcctgcgcg
     7261 cagtgcaggt gagcccatat cgagaccacc acgaagtaaa gcaactcttg ttacaaattt
     7321 acttttgaaa ccttttggtc tcgactgctt gcgtctcgac caacggatta cttctgaaat
     7381 tagaaaacat atgccaaaaa cactcttcga aaaaatctgg gactcacatg tagtcacaga
     7441 acaaccagat actccagcag tactctatat cgacttgcac cttgtgcatg aagtcacttc
     7501 accgcaggcg ttcacgggtc tgcgtcagcg agggcttaag gtacgtcgcc ctgagaagac
     7561 acttgcaacc atggatcatt ccatcccaac gacaccgcta aacgtgccga tcgccgatgc
     7621 catg
//
LOCUS       DASTJP010000607         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1210550, whole genome shotgun sequence.
ACCESSION   DASTJP010000607 DASTJP010000000
VERSION     DASTJP010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210550"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1362)
                     /locus_tag="VFQ23_17875"
     CDS             complement(118..1362)
                     /locus_tag="VFQ23_17875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9908523.1"
                     /translation="MKQSTLEPGLLQTLRVYVVLIAVLLPITWRSFSPTLGVAGGLQQ
                     FVTPGMPLILLLLIYMWFPGLERRIGRLFVPIGYLLLAAQAILGNYLTLQWLVPPPMH
                     DFAAVTLMLRTWVMIQFLVLFVAWQYNLFWMMVAAIGFSLLDATLYFPFVKESTAFYP
                     IYSALVVARFMGVTGVGLGFAWLITRQREHRAALAAANQKLAQYAATTEQLAVSQERN
                     RLARELHDTLAHSLSGATVQLEAVQALWDVQPNEARQILDQALEVTQNGLTEARRALQ
                     SLRASPLDDLGLSLSLSDMAKSTAARAGLRLELNVHNHLKNLTPDVEQCVYRVAQEAL
                     TNATRHADAKTLRVALQHDASCLTLIVADDGRGFDTAQVNDARYGLKGLHERAEMIGA
                     TLDVDSTLTTGTTIKLVVPLVE"
     gene            1598..2857
                     /locus_tag="VFQ23_17880"
     CDS             1598..2857
                     /locus_tag="VFQ23_17880"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9908524.1"
                     /translation="MKTFLFKFSQFTMIFLILMSFLGQAPAAVEAAPKPSNTPRPTNT
                     SRPTKTSRPTNTPRFTFTPTLTSSPTATTTFTNTPTLTFTPTDLPTDTPTLTFTPTAT
                     PAEPNLLAYWKLDEGTGNTAVDDSSNGHTLQLTPWGNWTNSSAPTGFDNPNALDIRYA
                     CSSDLGCKDYHSVDAINLANQSFTVAMWFRRYIDSNNAAFFFRLGSAPSAYQTFHMGF
                     RSSTIFTCGFWADDLDVSGSFTNTDWHHYACSYDAATNTRKAYVDGLLIGSDHPAASF
                     QGSGPVRLGTDLSFLSADDVRLYGNALSDAQVFALVPRSAPCEINADVWPRSGLKIPQ
                     LLQLAAADTNQCSVPLEYHWECLSDTSVECAAFLSEANAGPHGIATPMLNLQEFDVIS
                     IQVNVCEVGNPSNCSSLWRLYEGVPVD"
BASE COUNT          698 a          844 c          726 g          620 t
ORIGIN      
        1 ctggcgatag agcaacttcc cttgcaagaa atattcctct gacctgttgg tctcggtacg
       61 agcgagaaaa gcactcgctc tactcgacca gcgagtgtcg tgtcgagacc acaagttcta
      121 ctcgaccaac ggaacaacca attttatggt cgtccctgtt gtcaaggtgc tgtctacatc
      181 cagagttgca ccgatcatct cggcgcgttc gtgcaaaccc tttaatccat atcgagcgtc
      241 attgacttga gcagtgtcga agccgcgccc atcgtcggca acaatcaggg tcaaacagct
      301 cgcgtcatgt tgaagagcca cacgcagtgt tttcgcatct gcatgacgcg tggcattggt
      361 gagcgcctcc tgtgccacgc ggtagacaca ttgttctaca tctggcgtca ggttcttaag
      421 atgattgtgc acattcaact cgagacgcag gcctgcgcgc gccgcagtcg actttgccat
      481 atcgctgagg gagagcgata accccaaatc atccagcggg ctggcacgca gggattggag
      541 tgcgcggcgc gcttcagtga gtccattctg cgtgacttca agcgcctggt ccagaatttg
      601 acgcgcttca tttggttgca catcccataa agcctgcaca gcctccaact gcaccgtggc
      661 gccgctcaga gagtgagcca atgtgtcgtg cagttcacgt gccaggcggt tgcgctcctg
      721 gctcacggca agctgttctg ttgtcgctgc atactgagcc agtttctgat ttgccgctgc
      781 cagcgcggct cggtgctcgc gctggcgcgt gatcaaccag gcaaacccca accccacccc
      841 ggtcaccccc ataaaacgcg ccaccaccag agcggaatag atggggtaga aagccgtgct
      901 ctccttcacg aaggggaaat agagggtcgc atccagcaga gaaaacccga tcgctgccac
      961 catcatccaa aacaagttgt attgccaggc aacaaatagg accaaaaact ggatcatgac
     1021 ccaggtacgc agcatcaatg tcacggctgc aaagtcgtgc atgggcggcg gcaccagcca
     1081 ttgcagggtc agataattcc ccaggatcgc ctgtgctgcc agcaaaaggt acccgatcgg
     1141 cacgaaaagg cgtccgatgc gccgctccaa cccgggaaac cacatataga ttaacagaag
     1201 cagaatcagg ggcatgcccg gggtaacaaa ctgctggagc ccgcccgcca ccccaagggt
     1261 tggactgaag gaacgccagg tgattgggag cagaacggcg atcagaacca cgtacacgcg
     1321 cagcgtttgc aacaaacccg gctcgagcgt tgattgcttc atcgcaaaca tcatagcaca
     1381 acttgatatg ggaactgata tgacgcgcgt cacctgtgtg gatgacacgc gtcatatcag
     1441 gaggggcacg atgtcatacg gctttatgca cgcggtcact agctcgccgg gctggtttcc
     1501 agtatcgtag caatgttgga taaccaagcg aatatcaaat tcaaattaaa cagtaaccaa
     1561 aggtgacagt caccttttat gcaaagagga gcttgctatg aaaacatttt tattcaagtt
     1621 cagccagttt acgatgatct ttttgatcct gatgtctttt cttgggcagg caccagccgc
     1681 agtggaagcc gctcccaagc caagcaatac gccgagacca acgaatacct cgaggccgac
     1741 caaaacatcc aggccgacga atacccccag gttcacgttt acaccgacgc tcacatcctc
     1801 accgactgcc acgactacct tcacgaacac accgacgctc accttcaccc ccacagacct
     1861 tcctaccgat acgcccactc tgacatttac cccaaccgct acgcctgcgg aacctaactt
     1921 gcttgcctac tggaaactag atgaaggcac cggcaataca gcggttgatg attcgagcaa
     1981 tggtcatacg ctgcaattaa caccgtgggg aaattggacg aacagcagcg cgccgaccgg
     2041 cttcgacaac ccgaatgccc tcgacattcg ttatgcatgt tcgtcagatt tgggatgcaa
     2101 agattatcat tcggtggacg caattaacct ggcaaaccaa tccttcacag tagccatgtg
     2161 gtttcggcgc tatatcgata gtaataatgc cgcgtttttc ttcaggctgg ggtcagcgcc
     2221 cagcgcctat caaaccttcc atatgggctt tcgctcttcg accatcttta cgtgcggttt
     2281 ctgggcagac gatctcgacg tatccgggtc cttcacaaac acggactggc atcattatgc
     2341 ctgcagctac gacgccgcca ccaatacccg taaggcctac gtggatggtc tgctcatcgg
     2401 cagcgaccac cccgccgcca gctttcaagg ttcaggcccc gtgagactgg gaactgattt
     2461 aagctttctt tctgcggatg acgtacgact ctacgggaat gccctcagcg atgcgcaggt
     2521 ttttgccctg gtgccaagaa gtgcgccctg tgagatcaat gcagacgtat ggccgcgcag
     2581 cggtctgaaa attccacagt tgctccaact ggcagccgca gacaccaatc aatgcagcgt
     2641 tcctttagag tatcactggg agtgtctaag cgatacgagt gttgagtgtg ccgctttcct
     2701 gtctgaagcc aacgccggac cccacgggat tgccactcca atgttgaatc tccaggagtt
     2761 cgatgtcatc agcatccagg tcaacgtctg cgaggttggt aatccttcca actgttcctc
     2821 tctgtggaga ttatacgaag gggtacctgt ggactaaaag gatcctcaag tagcctctcc
     2881 ccacatcc
//
LOCUS       DASTJP010000608         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1048091, whole genome shotgun sequence.
ACCESSION   DASTJP010000608 DASTJP010000000
VERSION     DASTJP010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048091"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VFQ23_17885"
     CDS             <1..1318
                     /locus_tag="VFQ23_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET9908525.1"
                     /translation="IYPAKQYLTDSDRLKDAIHNIEAELEERLAYFKETGKYLEAQRL
                     EQRTRYDLEMLKEVGYCSGIENYSRHLDHREPGTHPWTMIDFLPNDYLLVLDESHMTV
                     PQVRGMYNGDRARKETLVEYGFRLPSAMDNRPLKFDEFEEVMGHTIYTSATPAAYEME
                     HAQQVVEQIIRPTGLIDPEVEVRPTKGQVDDLVGEIRQRVDNGERVLVTTLTKRMSED
                     LAEYLKELSFKVHYLHSEVETLERIGILRDLRLGVFDVIVGINLLREGLDLPEVSMVA
                     ILDADKEGFLRSGTALIQTIGRAARNVNGRVIMYADKMTDSMKRAIDETNRRRAKQVK
                     YNEENGIVPISIHKAIHDLTEELTVKSVGEEKGDYRVKSVGGIPRNELRQIVFEVEKQ
                     MKEAAKNLDFERAAALRDELYELKNLLAEDESLKPWERIKLLTGEE"
     gene            1394..1885
                     /locus_tag="VFQ23_17890"
     CDS             1394..1885
                     /locus_tag="VFQ23_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908526.1"
                     /translation="MTILGIVVFVCHSWALLGFFKRLSSFILYIELAEIAGIFAFMMA
                     FAFLESLLITGILVLLSAILPLTWLRSGFAFKGFIVIVMATISALLLQRTMQGTLPAL
                     PILRLIWFTPFVWTIIFIILAQRKPGVQSVLLDIAERISIMLFVYGPVGLISLFVVTL
                     GYL"
     gene            1891..>2242
                     /locus_tag="VFQ23_17895"
     CDS             1891..>2242
                     /locus_tag="VFQ23_17895"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9908527.1"
                     /translation="MQEFNGLNRRDFLKLISSIPVGIFSRSISKVTQTADTPNVIIIV
                     FDAWSQRHTSLYGYLRPTMPNLEKFAEKATVYHNHYSAGTFTTPGTASLLTGLYPWSH
                     RAMHLGAALTPLHAG"
BASE COUNT          554 a          527 c          592 g          569 t
ORIGIN      
        1 gatctaccct gccaagcaat atttaacgga cagtgaccgg ttaaaggatg ctatccataa
       61 tattgaagcc gagctggaag agcgattggc atacttcaaa gagacgggca aatacctcga
      121 agctcagcgc ctcgagcagc gtactcgcta cgatctggag atgttgaagg aagtgggcta
      181 ttgttcgggg atcgagaatt attcacgcca tctcgaccat cgcgaacccg gaacccatcc
      241 atggacgatg attgacttcc tgcctaatga ttacttgctg gtgctggacg aaagccacat
      301 gacggtgcct caggtccgcg gcatgtataa cggagatagg gcacgtaaag aaactcttgt
      361 tgaatatggc ttccggttac caagtgcaat ggataatcgt ccgttgaaat tcgatgagtt
      421 tgaagaagtg atggggcaca ccatatacac ctccgctacg ccggcggcat acgaaatgga
      481 gcacgctcag caagtggtgg agcagatcat tcgtccgacg ggattgatcg atcccgaagt
      541 tgaagtgcgg ccgaccaaag gtcaggtaga cgatctggtg ggagagatca ggcagagggt
      601 cgataacggc gaacgcgtcc ttgtcactac gctgacgaaa cgtatgtcag aggatctggc
      661 tgagtattta aaggaactga gcttcaaggt ccattatctg cattccgaag tggaaacatt
      721 ggagcgcatt ggtatcctgc gtgacctgcg cctgggtgtg ttcgatgtga tcgtcggaat
      781 caaccttttg cgagagggtc tcgatctgcc tgaagtatcc atggttgcta tcctggatgc
      841 ggataaagaa ggttttctgc gctcaggcac cgcactcatt cagacgatcg gtcgggccgc
      901 gcgaaacgtc aacggccggg tgatcatgta cgccgacaag atgaccgact caatgaaacg
      961 tgccatcgat gaaaccaatc gtcgccgtgc caagcaggtg aaatataatg aagagaacgg
     1021 catcgtgccg atcagcattc ataaggcgat ccatgacctg actgaagaat tgaccgtgaa
     1081 atcggtgggt gaggaaaaag gtgattaccg ggtcaagagt gtgggtggta tcccacgtaa
     1141 tgaattgagg cagatcgtct ttgaagtgga aaaacagatg aaggaagcgg caaagaatct
     1201 ggatttcgag cgagccgctg cgcttcgtga cgaactttat gaactaaaga atctgctcgc
     1261 cgaagacgag tccctcaagc cttgggaaag aatcaaattg ctgacggggg aggagtagcc
     1321 atctcccttt ccatgaaagg gtagggaacc gctgaactac cttcgtagtc gttttcctgc
     1381 tcgaaatgaa atcatgacga tcctgggtat cgtcgttttc gtctgccata gttgggctct
     1441 gctaggcttc ttcaaaaggc tttcatcttt catcttgtat attgaactgg cggagatcgc
     1501 agggatattc gcattcatga tggctttcgc attccttgag agtttgctga tcactggcat
     1561 actcgtgctg ttgagtgcga tcctgccatt gacttggttg agaagtggct ttgcctttaa
     1621 aggatttatc gtcatcgtga tggcaaccat ctctgcattg cttctccaaa gaaccatgca
     1681 gggaacgttg ccggcactgc cgattctgag gttgatttgg ttcacgccat tcgtatggac
     1741 catcatcttc atcattcttg cacagcgaaa gccaggggtt caaagtgtcc tgctcgatat
     1801 cgcagaacgc atttcgatca tgctatttgt ttatggtccc gtgggcttga tttctctttt
     1861 cgttgttacg ctgggatatc tttagtcgcc atgcaagaat tcaacggttt aaatcgacgt
     1921 gattttctga aattaatttc atcgattccg gttggcatct tttcccgttc catttccaaa
     1981 gtaacccaaa ccgcagatac cccaaacgtt atcatcattg tgttcgatgc ctggtcacag
     2041 cgacatacct cgctgtatgg ctacctacgg ccgaccatgc ctaaccttga aaagtttgcc
     2101 gaaaaggcta cggtctatca caaccattat tctgcaggga cgtttacaac tcctggaaca
     2161 gcgtcgcttc tcactgggtt gtatccctgg tcgcaccgag ccatgcatct gggagcggcg
     2221 cttacgcctc tgcatgctgg tc
//
LOCUS       DASTJP010000609         8964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1117874, whole genome shotgun sequence.
ACCESSION   DASTJP010000609 DASTJP010000000
VERSION     DASTJP010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117874"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFQ23_17900"
     CDS             complement(<1..596)
                     /locus_tag="VFQ23_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9908528.1"
                     /translation="MQEVTDVQRLSKRVAGLKPSGIRKFFDIAATMKDVISLGIGEPD
                     FTTPKPILEAGIRSLKNGETHYTSNWGKMELRQAIADNLKRLYQVQYDPNDEIIATVG
                     VSEALYLAFTAILDPGDEVIIPTPCFVSYQAEVIMAGGVAVEVPAREENDFMVDPADI
                     RKALTPRTKVIFIGYPSNPTGAVATREVMLEIAKIAEE"
     gene            complement(611..823)
                     /locus_tag="VFQ23_17905"
     CDS             complement(611..823)
                     /locus_tag="VFQ23_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908529.1"
                     /translation="MIDTVECRSDSEYAERPLSLTWEGARYEIAEIVARWRGPAERGF
                     RVKTTDGQAFDLTYRELPDEWIVQPI"
     gene            complement(826..1854)
                     /locus_tag="VFQ23_17910"
     CDS             complement(826..1854)
                     /locus_tag="VFQ23_17910"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9908530.1"
                     /translation="MTDTLKIFIPMAGWGTRMRPHTHSKPKPLVSVAGKTSLEHLMDM
                     FKTLPEGQVVEYVFIVGPFLGEKQIPAFIEENFPNLKAHYVIQNEMKGQSHALWLARE
                     HMHGPMLMCFSDTLMETDFSFLADEEADVVSWVMPVPDPRRFGVAEVGEDGWVKRFIE
                     KPQSMENNLVVVGCYYFKSAENLLAAIEDQMQRGLMLKGEYFLTDTISLMIEGGAKVR
                     TESIGTWLDTGTIEATLDTNKLLLDKLGSSIGKFKDSNVEIIEPVAIHETAEISNSKI
                     GPHASIGANCKISNSSIAESIVEAGSEIQDAALTRSLIGKQAKVRGGGDGQSAQLNIG
                     DNSEVIIK"
     gene            complement(1934..2917)
                     /locus_tag="VFQ23_17915"
     CDS             complement(1934..2917)
                     /locus_tag="VFQ23_17915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9908531.1"
                     /translation="MQYVNLGKTGMKVSRLCLGMMSYGSKKWREWVLEEEEAKPFIKR
                     ALEAGINFFDTANVYSLGESERVTGNIFKGLGVRRENIVLATKVHGQMSDDVNDRGLS
                     RKHILDSIDKSLQRLQMDYVDLFQIHRWDSSTPIEETMEALNDVVRAGKARYIGASSM
                     FAWQFAKALHTSEKHGWTKFISMQNHYNLVYREEEREMIPLCQDQGIGLIPWSPMARG
                     FFAGDRKRDGGGETVRSNSDPFGKTLYFRDEDFTVAERAAEVAKSRGVTASQVALAWV
                     LNKPYVHSPIIGTTKMEQLDQALSALDIKLSDEEVKNLEEPYIPHPVLGHS"
     gene            complement(3056..5245)
                     /locus_tag="VFQ23_17920"
     CDS             complement(3056..5245)
                     /locus_tag="VFQ23_17920"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9908532.1"
                     /translation="MDFLDKLNKQQHSAVTAGNGPVLVVAGPGSGKTRVLTQRIAYLI
                     AVEGVRPWQILAVTFTNKAAKEMEARVKSLLPDQAIEGIMLGTFHSICARILRRESEH
                     LPIESNFVIFDSDDQERIVKSVIRELNINEKLYRPASVHGAISRAKNELIGADEYPTT
                     TYRDEVVKRVYVEYQKRLISSNAVDFDDLLVYTARLLEDNPNVRDKYAQRFRHVLVDE
                     FQDTNLAQYALVRQLASFNRNIFVVGDPDQSIYAWRGADWRNLQRFEEDFPDSQTVLL
                     EQNYRSHQNILDAAMGVINRARNRRRKQLFTERGKGEKIFFYEAPDDYAEASFVVDGI
                     AQLVASRQFEPGDCAVMYRTNAMSRLLEEAFLQARLPYRLVGAQRFYGRREVKDIISF
                     LRLVHNPADEASLDRVINVPPRGIGDKTLLTLHNVARLHNISPGMVLLDLARGAHSPY
                     WSSFTGRATISLADFGGMLATWRAAAPSLTTAELFDRIVTDLNYKEYVNDDSEEGTDR
                     WENVQELRRLAVEYSTRSLDEFLENVALVSDQDTLTEGNVPTLLTLHAAKGLEFGAVF
                     IVGLDDGILPHSRSFDEPEQMEEERRLFYVGITRAKDKLYLARAIQRGGRGMAEETYP
                     SRFLDDLPADLLVGKTRTGRAMRGAPAETKWQSYRTQLPSGDDARKRASVMETKFRAG
                     TRIQHQIWGDGIVLDSKVQDDDEIVDVVFESVGIKRLSASLANLKII"
     gene            complement(5311..5739)
                     /gene="mscL"
                     /locus_tag="VFQ23_17925"
     CDS             complement(5311..5739)
                     /gene="mscL"
                     /locus_tag="VFQ23_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET9908533.1"
                     /translation="MLKEFKEFVMRGNVLDLAVAVIIGGAFGKIVTSFVNDILMPPIG
                     LMLGGVNFADLFIALDGNAYESLEVATTAGAPTLNYGVFLNTVIDFLIVAFVIFLIVR
                     AANQMKKPAPAAEAVTKDCPHCFTAIPIKATRCPNCTSQL"
     gene            6175..8064
                     /locus_tag="VFQ23_17930"
     CDS             6175..8064
                     /locus_tag="VFQ23_17930"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9908534.1"
                     /translation="MYKNRFTLALGLLVVASMVFSACAPAPTAPPAEPAEPVVVTQEV
                     VVTEVVEVEREAFTTPHPILGDLNVRQALSYCTNKADLIKSVYVTLDEEQQAGLVMHT
                     NIPRDHWAYAGDENITIYPYDPAQGAALLDEAGWTLAEGANYRTNADGEELALKFTTT
                     NAAFRQTWSAVWIQQMADCGINVVATYAPASWWFGDTTGLARRDFELGAFAWVGEADP
                     DVQTLYACDQIPSPANGWEGQNYMGWCNEAASVAVKNGANTLVQDERAAFYTAFQAEF
                     TKDIPSIPLFNRTETHATNAALTGYNPRPGEPYYTYNIYDWEIPGTDTIVMGFPQEPA
                     SLFVLVEDAFVANVAYTLIRPNLITWLDYTAAAALIKELPTIESGLTLNNDVEVSEGA
                     KVVDANGDVVDLAPGVSVRNAAGETVEFTGGSVTMKQLVSTFELIDGLTWEDGTPVSQ
                     ADLELGYAIDCDPESGATSFILCDKTESVAVDGTSWTYTWLPGNQDPLYYESGLLSLY
                     PAHVLGDVPASEWATNPLVAETPLAYGPYKIVEWVKGERIVYAANEFWAGTPVKTPNI
                     VIQIITPESQESLLLGGEVDTLASEAVVSPTETLAAAATEGRIKLFVEPSATWEHIDL
                     SLFIR"
     gene            8137..>8964
                     /locus_tag="VFQ23_17935"
     CDS             8137..>8964
                     /locus_tag="VFQ23_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908535.1"
                     /translation="MGAFFIRRIIQMILVVLISAVASYALLNLAPGGPLAGLRQTQQN
                     ARFQITEDDIARIRAYFELDLFLPYRFTRWLIGVPRGPLEIGGRQYFSDVVVGCRKPV
                     ETDVLNEATGEFEIVTTGCNEAVQMKDLVGRRTSRGILLGDFGLSWRILRDRPVSDLL
                     LSRLPKTIQLIGLSTLLSLLIAIPLGIYSAVRQYSRFDYIFTTLAFIGSAMPVFFFGL
                     LMIIVFSLVPKEAGWPYVPAGLSESVRDYTIPLIGQVDAGSFKDRILHLILPTAVLTF
                     "
BASE COUNT         2104 a         2358 c         2172 g         2330 t
ORIGIN      
        1 tgttcttccg cgatcttagc gatctcgagc atcacttcac gcgtcgccac ggcaccggtg
       61 gggttggaag gatacccgat gaagatcact tttgtgcgcg gggtaagcgc cttgcggata
      121 tcggcagggt cgaccatgaa atcattttct tcgcgagccg gcacttccac agccacgccg
      181 cccgccatga ttacttctgc ctgatacgaa acaaaacagg gtgtcgggat gatgacctca
      241 tcgcccgggt caaggatggc agtgaatgcc agatacaatg cctccgaaac accgaccgtt
      301 gcgatgatct catcgtttgg gtcatactgt acctgataca agcgcttcaa attgtcagca
      361 attgcctgac gcaattccat cttcccccag ttggatgtgt aatgagtctc accgtttttc
      421 agtgaacgta tccccgcttc aaggatgggt ttgggtgtag tgaaatctgg ttcgccgatg
      481 ccaagcgaga tcacgtcttt catggtcgcg gcaatgtcaa aaaacttgcg aatgcctgag
      541 ggcttgagtc ctgctactct ttttgacaga cgttgaacat cagtcacttc ctgcattatg
      601 cctccatttt ttaaattggt tgtacgatcc attcatcggg tagttcccga taggtcagat
      661 caaacgcttg cccatccgtt gtcttcaccc ggaatcctct ttcagcagga ccgcgccagc
      721 gtgcgacgat ctccgcgatc tcataccgag cgccctccca cgtgagggaa agggggcgtt
      781 ccgcatattc agaatccgag cgacattcga ctgtatctat catgattact tgattatgac
      841 ttcgctatta tcaccgatat tgagctgggc gctttgccca tcgcctccac cacggacttt
      901 ggcttgcttg ccgattagag agcgtgtcaa cgctgcatct tgaatctcac tacctgcttc
      961 gacgatgctt tctgcgatgc tgctattgct tattttacaa ttcgcaccaa tagatgcatg
     1021 cggaccaatt tttgaattcg aaatctccgc tgtttcatga attgcaaccg gctcgatgat
     1081 ttcgacattt gaatctttga attttccgat actcgaacct aatttatcca gcaataattt
     1141 atttgtatcc aacgtcgctt cgattgttcc tgtatcgagc caggtgccga ttgactctgt
     1201 ccgcactttt gctccaccct caatcatcaa ggagatcgta tccgtcagga agtattcacc
     1261 tttgagcata aggccgcgct gcatctgatc ttcaattgcc gccagcaggt tttctgcgct
     1321 cttgaaatag tagcaaccta ccaccacgag gttattttcc atggattgcg gcttttcgat
     1381 gaagcgtttc acccaaccgt cttcacctac ctctgccaca ccaaaacggc gcgggtccgg
     1441 gacgggcatg acccaggaaa caacatctgc ctcttcatcc gcgagaaacg agaaatccgt
     1501 ctccatcagt gtatcagaga aacacatcag catgggtccg tgcatatgtt cacgtgccag
     1561 ccaaagcgca tgcgattgtc ccttcatctc attctgaatg acatagtggg ccttgagatt
     1621 cggaaaattt tcttcgatga acgcggggat ttgcttttcg cccagaaacg gacccacgat
     1681 aaagacatat tcaacaacct gcccttccgg gagggtcttg aacatgtcca ttaggtgttc
     1741 aagcgaggtt ttcccagcca cactgacgag tggcttgggc ttactgtgtg tatgcggtcg
     1801 catgcgcgtc ccccagcccg ccattggaat gaaaatttta agggtgtccg tcactgaacg
     1861 ctccgtattc aatggtgtag gctgttattt taccaatgaa aggagcgttg tacgattttc
     1921 ctgttacatt ttcttacgaa tgtcccaaaa ccggatgggg aatataaggt tcctcaaggt
     1981 tcttcacttc ctcgtccgaa agcttgatgt ccaaggcaga gagagcctga tcgagctgct
     2041 ccattttggt tgtaccgata atgggactgt gaacgtatgg cttgttcaaa acccacgcca
     2101 gggcgacctg cgaagccgtc acgccgcgtg attttgcgac ttctgcggcg cgctctgcca
     2161 cggtgaaatc ttcgtcgcga aaatataagg ttttgccaaa tgggtcgcta ttcgagcgga
     2221 ccgtctctcc gccaccatcg cgttttcggt caccggcaaa aaagccgcgt gccatcgggc
     2281 tccaggggat aagtccgatg ccctgatcct gacataatgg gatcatctca cgttcctctt
     2341 cacggtagac aaggttatag tggttttgca tcgagatgaa ctttgtccag ccgtgtttct
     2401 cagaagtatg cagcgccttg gcgaattgcc aggcaaacat cgaagaggcg ccaatataac
     2461 gcgctttgcc agcgcgcacc acgtcgttta aagcttccat ggtttcttca atcggagtgg
     2521 aagaatccca gcgatgaatt tggaaaaggt caacgtaatc catttgcagg cgttgaaggg
     2581 atttgtcaat cgaatccagg atgtgttttc gcgaaaggcc gcgatcattt acatcatcac
     2641 tcatctgtcc atggaccttg gtcgcaagga caatgttctc gcgcctgacg cccaaccctt
     2701 tgaagatatt tcctgttacg cgttcacttt cgcccagtga atagacattt gcagtatcaa
     2761 agaaattgat ccctgcctca agcgcgcgtt tgataaacgg tttagcttcc tcttcctcca
     2821 gaacccattc ccgccatttc ttcgacccat agctcatcat gcctaaacat aaacgcgaga
     2881 ctttcattcc agttttgccg aggttgacgt attgcatgcg gactccttta cgctcccttc
     2941 gccgtcctcg gcgaaggagc tgatattagc cattcacgga tatctgtaat atcaattatt
     3001 ggttttcttc catgtaatta cgtcaccgcc gaggacggcg gtgaaagcgc agcggctaaa
     3061 ttatcttcaa attcgccaga cttgcagaaa gccttttgat cccaaccgat tcaaacacca
     3121 catctacaat ctcgtcgtca tcctggactt tgctatcgag gacaatccca tcgccccaga
     3181 tttgatgctg gatgcgcgtt ccagcgcgga actttgtttc cataacggag gcacgcttac
     3241 gggcatcatc gccagatgga agctgagtac gatacgactg ccatttcgtt tcggctggcg
     3301 cgccgcgcat ggcgcgtccc gtccgtgttt tgcccactaa tagatctgcg ggcaggtcat
     3361 ccagaaatcg ggatggatag gtctcttccg ccatgccacg tccaccacgc tgaattgcac
     3421 gagcaagata gagtttatcc ttggcgcgtg tgatgccaac atagaaaagg cgtcgctcct
     3481 cttccatctg ttctggctca tcaaacgaac gactatgcgg caagatccca tcgtcgagtc
     3541 caacaatgaa gactgcgcca aactccagtc ccttggcggc gtggagggtg agtagtgttg
     3601 gaacgttgcc ttctgttaat gtgtcttgat ccgagacgag cgcgacattt tcgagaaatt
     3661 catcgagaga gcgcgtggaa tattcgaccg caagacgcct caactcctgc acgttttccc
     3721 accgatcagt tccctcctct gagtcatcat taacatactc tttgtagtta aggtccgtta
     3781 cgatacggtc gaataactcg gcggttgtta atgagggcgc tgccgcgcgc caggtagcga
     3841 gcatcccacc aaagtccgcg agggagatgg ttgcgcgtcc agtaaaagat gaccagtacg
     3901 gtgaatgggc tccgcgagcc aggtcgagga gcaccatgcc ggggcttata ttatgcaggc
     3961 gcgcgacgtt gtgaagtgtg agtaaagttt tatcaccgat gccgcgtggt ggcacattga
     4021 tcacacgatc cagactggct tcatcggcag ggttatgaac aagacgtaga aatgaaataa
     4081 tatccttgac ctcgcgccgg ccatagaacc gctgcgcgcc caccaaacga taaggaagcc
     4141 gtgcctgaag gaaggcttct tccaacaaac gggacattgc attggtacga tacatcaccg
     4201 cacaatcgcc cggctcaaat tgacgcgaag caaccagctg tgcaatacca tccacaacga
     4261 aggaggcctc tgcataatca tccggtgctt catagaaaaa tattttttcg cccttgcctc
     4321 gttcggtaaa aagctgcttg cgcctgcggt ttcgagcgcg gttgatgact cccatagctg
     4381 cgtcgaggat attttgatgc gaacgataat tctgctcgag caaaactgtt tgcgaatctg
     4441 ggaaatcctc ttcaaagcgc tgcaggttac gccaatcagc accgcgccag gcatagattg
     4501 attggtcagg gtcgccaacc acgaagatgt ttctattgaa ggaagcaagc tgtcttacaa
     4561 gtgcatattg cgccagattg gtgtcctgaa attcatccac aagcacatgg cgaaatcgtt
     4621 gtgcgtattt atcgcgcaca tttgggttat cttccaataa gcgcgccgta tatacaagca
     4681 agtcgtcgaa atccaccgcg ttgctggaga tcaatcgctt ttgatattcc acatacacgc
     4741 gcttgaccac ttcatcgcga tatgttgtgg tcggatattc atccgctccg atcagttcgt
     4801 tcttggcacg cgagatcgcg ccatgcacac tggctgggcg gtataacttt tcattgatgt
     4861 tcaactcgcg aatgacgctc tttacaattc tttcctgatc atccgaatca aagatcacaa
     4921 aattcgactc gatggggagg tgttcggact ccctcctcag gatgcgggca cagatcgagt
     4981 gaaatgtgcc gagcatgatg ccctcaattg cctgatctgg caaaagggat ttaacgcgcg
     5041 cctccatttc cttcgcggcc ttgttggtga acgtcacagc caggatctgc cacgggcgga
     5101 cgccttccac cgcgatcaaa taggcaatac gctgcgtaag tacgcgcgtc ttaccggacc
     5161 ctggaccggc tacgactaaa acaggtccat tcccggccgt gaccgcgcta tgttgttgtt
     5221 tgttgagttt gtcaagaaag tccatggaaa tagaaagtag agagtagtct atcacaacta
     5281 ctctctactt tgaattaaat tgagttttct ctaaagttga gacgtgcagt tagggcagcg
     5341 cgtggcttta atggggatgg ctgtgaaaca atgtggacaa tctttcgtaa cagcttccgc
     5401 tgcgggagca ggctttttca tctggttggc tgctctcaca ataaggaata tcacgaaggc
     5461 aacgatgaga aaatcaatca cggtattcag aaacactccg taattgagcg tcggcgctcc
     5521 ggcggtagta gccacttcga gcgactcata cgcatttcca tccaacgcga taaacaaatc
     5581 ggcaaaatta acgcctccca gcatcaaacc aatgggaggc atcaaaatgt cattcacgaa
     5641 tgatgtaacg atcttgccga atgcgccacc gatgatgact gcaaccgcca gatccagcac
     5701 gttgccgcgc atcacaaatt ccttgaattc ttttaacatt ctccactctc cttttccgat
     5761 ttagatcaaa gttattctaa tatctggaat gaggtatgtc aacgcacgcc gaattcccat
     5821 cctgcagaag gtcgtgttta aggattacac gaagcctcca gtcctttaga gttgttttca
     5881 gcagttcttt ggaactgttt tttagcaaat ttgtctgaat aactaccctt ccagagtatt
     5941 gctgttgacg ctctcaaaga attaggtata atttactcgc tcttacaaat atttaatgtt
     6001 acgggcaaca tcatttgtaa aaaaatttcc atgagatatc caatatttta aggttgttgc
     6061 ttatgaccaa taacttctct tcaagatgaa aggaggtgga gcgggcaata ttctgcatga
     6121 tagcaagatt agttgaaaat ccaatttaaa ccttttgtag tgaggagagt ttacatgtat
     6181 aagaatcgct ttacgctggc cttgggcctg ctggtcgtag ccagcatggt cttttcagct
     6241 tgcgcccccg cccctactgc cccgcccgct gagcccgccg aacctgtcgt ggtgactcag
     6301 gaagttgtgg taacagaggt ggttgaagtt gaacgcgaag cgttcaccac gccgcaccca
     6361 atcctgggcg atttgaacgt tcgtcaggca ctttcatact gcaccaacaa agccgacttg
     6421 atcaagtctg tatatgtgac tttggacgaa gagcaacagg cgggcctggt catgcacacc
     6481 aatatccctc gggaccattg ggcttatgct ggtgatgaga acatcaccat ttacccgtat
     6541 gatcctgctc agggtgccgc tctgctcgat gaagccggct ggacgcttgc tgaaggtgca
     6601 aactatcgca ccaacgccga tggtgaagaa cttgccctga aattcacgac caccaacgcc
     6661 gccttccgcc agacctggtc cgccgtttgg attcagcaga tggctgactg cggtatcaat
     6721 gtggttgcca cgtacgcccc tgcctcctgg tggttcggcg atacaaccgg tctcgcacgc
     6781 cgcgattttg aattgggcgc ctttgcctgg gtaggcgaag ctgaccccga tgtgcaaacc
     6841 ctgtatgcct gtgatcagat tccttcgccc gccaatggtt gggaaggtca gaactacatg
     6901 ggctggtgta acgaagctgc cagtgttgca gtcaaaaatg gtgccaatac tttggtgcag
     6961 gatgagcgag cagcgtttta cactgcgttc caggctgaat tcaccaagga tattccgagc
     7021 attcctctct tcaaccgcac agaaacccac gccaccaacg cggctctgac tggttacaat
     7081 cccagacctg gtgaaccata ctatacgtat aacatttacg actgggaaat ccccggcacc
     7141 gacacgatcg tgatgggctt cccgcaggaa cccgcctcgc tcttcgtgct cgtggaagat
     7201 gcctttgtgg caaacgttgc ctacaccttg atccgcccga atttgatcac ctggctggac
     7261 tatacagccg ccgcggcttt gattaaggaa ttgccgacga tcgaaagcgg cctcacgctc
     7321 aacaatgatg ttgaggtttc tgaaggagcc aaggttgtgg atgccaacgg cgatgtcgtt
     7381 gacctcgcgc cgggcgtttc tgtcagaaat gctgctggtg aaaccgtcga atttactggc
     7441 ggatctgtta ccatgaagca gttagtatcc accttcgaac tcattgacgg actcacctgg
     7501 gaagacggta cacccgtttc acaggctgac cttgaacttg gttacgcgat tgattgcgat
     7561 cccgaatccg gcgccacctc tttcatcctc tgcgacaaga ccgaaagtgt tgccgtagat
     7621 ggcaccagct ggacctacac ctggctgccc ggtaatcagg atccgctgta ttacgaaagc
     7681 ggattgctct ccctctatcc cgctcatgtg ttgggtgacg tccccgcttc tgaatgggca
     7741 accaatcccc tcgttgctga gacaccactc gcttatggcc cctacaagat cgtcgaatgg
     7801 gtcaagggtg aaagaattgt ctacgcagcg aatgaattct gggcaggtac acctgtcaag
     7861 accccgaata ttgtaatcca gatcattaca cccgaaagtc aggaatccct cctgctcggc
     7921 ggtgaggtcg atactctcgc ctccgaagct gtagttagcc cgactgaaac tttggccgcg
     7981 gctgccacag aaggccggat caagctcttt gtggaaccaa gcgcaacctg ggaacacatt
     8041 gacctttccc tgtttattcg gtagttttac gattgtagtg ctgcaaaatc ggtgtgcctc
     8101 gaaagaggca caccgatcta aataaagagg tgtcttatgg gtgcattttt catcaggcga
     8161 ataattcaaa tgatcctggt cgtgttaatc tcagcagtcg cctcttacgc gctactgaac
     8221 cttgcgcccg ggggaccgct tgctggtctc aggcagaccc aacaaaatgc acgttttcaa
     8281 attacagaag atgatattgc gcgcattcgc gcttattttg aactggactt atttcttcct
     8341 tatcgtttta cgcgctggtt gatcggcgtg cctcgtggac ccctggagat cgggggcagg
     8401 caatattttt ctgatgtagt ggttggatgt cgaaaacctg ttgaaaccga tgttttgaat
     8461 gaagcaaccg gggaatttga aatcgttacc acaggatgta acgaagccgt gcagatgaaa
     8521 gacctggttg ggcgacgtac aagtcgcggt atcttattgg gcgatttcgg tttgtcctgg
     8581 cgtatcctgc gtgaccgccc agtatcggac ctgctcctca gccgcctgcc aaagaccatt
     8641 cagttgatcg gcctttccac cctgctctca ctgctgattg caattccgtt agggatatat
     8701 tcagccgttc gacaatattc caggtttgat tatattttta caacgctggc gtttatcggc
     8761 tctgccatgc cggtcttttt ctttggtttg ttaatgatca tcgtattttc acttgttccg
     8821 aaggaagctg gttggccgta tgtgccggct ggtttgtctg aatcagtccg tgattacacc
     8881 atccctctca ttggacaggt ggatgcaggc agcttcaagg accgaatatt acacttgatc
     8941 ctgccaacgg ctgttcttac cttt
//
LOCUS       DASTJP010000610         4348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1442819, whole genome shotgun sequence.
ACCESSION   DASTJP010000610 DASTJP010000000
VERSION     DASTJP010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442819"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFQ23_17940"
     CDS             complement(<1..903)
                     /locus_tag="VFQ23_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9908536.1"
                     /translation="MNKWVYQFDEVTKAEKYAGSWEGVRSLLGGKGAGLFDMTRAGVP
                     VPPGFTVTTEACNHFRKTGKFPPGMWDQALAAMKLVEKATGKKFGDPKNPLLVSCRSG
                     AKFSMPGMMNTILNIGLNDKVVEGLEKLTNNPRFAWDSYRRLIEMFGTTVFNVDDEVF
                     EHPMAEYKAERGYKVDTEMTAEDWKALVSTFKSVYKQHIGSDFPQDVNKQLELAIKAV
                     FESWNGKRAIAYRKKEGISDDLGTAVNIVTMVFGNMGDDSGTGVAFTRNPSTGDKKMM
                     GEYLINAQGEDVVAGIRNAEPIENL"
     gene            1067..1480
                     /locus_tag="VFQ23_17945"
     CDS             1067..1480
                     /locus_tag="VFQ23_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9908537.1"
                     /translation="MTTKIFVIDDDSAVTDLLSVLLNTQGFQVWVTNSSSEGLTQIRE
                     KAPDLVILDLMMPEMDGWEVCKAVRAFSDVPIIVLSALNDPSMVASVLDAGADDYLTK
                     PTPSRVLVAHIKRLVDRKASTVSVNQNLPGTLPAS"
     gene            complement(1554..2318)
                     /locus_tag="VFQ23_17950"
     CDS             complement(1554..2318)
                     /locus_tag="VFQ23_17950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9908538.1"
                     /translation="MEKLYVNNLNLAYERRGSGSPLLLLHGFPLDHHLWDEVVPLLEN
                     SFDLIIPDLRGFGESETVDTPYTMDDYASDLAGLLDHLGIQKTAVAGHSMGGYVALAF
                     AKTYPERVSGLALVSSQTPADPPERKEGRYKTAADVAEKGIGGVVETMAPKFTGDPRL
                     QDAARKIMEKQQPTAYIGALKAMAERADTTATLNSFTFPLVVIHGDEDALIPVDRARE
                     IKASIPGSHYVELRGIGHVPMLEAAEKTAEALKQLS"
     gene            complement(2346..2879)
                     /locus_tag="VFQ23_17955"
     CDS             complement(2346..2879)
                     /locus_tag="VFQ23_17955"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET9908539.1"
                     /translation="MNTVYFDLESQKLFDEVGGRYPSKLLLACGVTWSTARKDFAVYW
                     EQDAAALVQELKSADRVIGFNIINFDYEVLRPYAPNVNLRGIRTIDMLADIYRTLNFR
                     LSLDSIAKATLGTTKSGDGVQSVRWFRNGELDKVAEYCKADVDITRRIHEFGSENGFV
                     HYYSRMGSKLKVEVNWK"
     gene            complement(2876..>4348)
                     /locus_tag="VFQ23_17960"
     CDS             complement(2876..>4348)
                     /locus_tag="VFQ23_17960"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9908540.1"
                     /translation="VDRVSEILLHQEIYLVGGAVRDMMLNRLSHDLDFALPSNGISTA
                     RRVANALKADFMALDEERDTGRVLVSEADGDRTFLDFATYRGANLEEDLRARDFTINA
                     IAYDVRSKTLLDPLNGTADLRLKLIRACSQTSISDDPVRILRAVRQAAAFSFKIELAT
                     RKSMKQAAHLLPNISPERKRDELFKILEGPKPDASIRALEMLGVFPHLLPELSALKGV
                     EQSSHHVYDVWEHTLKVLGYLEEIITTLGIKQAPPEEIDPFGALLIQHLGKYKERFTA
                     HFEKLLNTDRSVRAVLFFAALYHDVEKPATRSVDETGRIRFFEHEIKGAEVTSKRAQS
                     FNLSNDEVERVKKIVKNHMRFHFFTNQMQELKQEPSRRAIYRFFRDTGEAGIDLLLLG
                     LADLRGTRGNTLSQEAWTAALQVAELLLNNYWERPEQTISPPRLLDGNDLMRELNLEP
                     GRIVGQLLEEIREGQAIGNIETRDQAIEFAREHLKNLETS"
BASE COUNT         1065 a         1097 c         1002 g         1184 t
ORIGIN      
        1 tagattttca atgggttccg cattgcgaat accggctact acgtcctcac cctgcgcatt
       61 gatcagatat tcgcccatca ttttcttatc acctgtggat gggttgcggg tgaaagctac
      121 acctgtgccc gagtcatccc ccatgttacc gaagaccatg gtaacgatat ttactgcagt
      181 tccaaggtca tccgaaatgc cttccttttt gcgatatgca atcgctcgtt tgccattcca
      241 tgactcaaac actgccttga ttgccaattc gagctgctta ttaacatcct gagggaaatc
      301 tgagccgata tgttgtttat aaacggactt gaatgtggag accagggctt tccagtcttc
      361 cgcagtcatt tctgtatcga ccttgtagcc ccgctctgct ttatattcag ccatgggatg
      421 ctcaaagacc tcatcatcca cattaaacac ggttgtgcca aacatttcga tcagacgacg
      481 ataggagtcc caggcaaagc gggggttatt tgtaagcttt tccaagcctt cgacaacttt
      541 gtcgttcaac ccgatattta agatggtgtt catcatgccg ggcatcgaga attttgctcc
      601 cgaacggcag gaaaccagca gtggattctt cggatcgcca aatttcttgc cagtcgcttt
      661 ttcaacaagt ttcatggcgg cgagggcctg atcccacatt cccggtggga atttgcctgt
      721 tttccggaaa tgattgcagg cttcggtcgt gactgtgaat ccgggtggga cgggtacgcc
      781 tgcccttgtc atgtcgaaaa gtcccgcgcc tttgccgccc agtaaagacc gaacaccttc
      841 ccacgatccc gcatattttt ccgcttttgt gacttcgtca aactgataga cccacttgtt
      901 catggaaacc tccagggtaa tgttataatt ttctgcagaa gtttaccacg aacaatatag
      961 tacaaaagta atgtttttga aatttaactt tcgcctttat ctgttagcct attgcaaagt
     1021 aaaaaatgct aattatggat gataatggcg gaagatagga aaacacatga ccacgaaaat
     1081 ttttgttatt gatgatgatt ctgcggtgac cgacctgctc tctgtgcttt tgaacacgca
     1141 gggttttcag gtgtgggtca caaatagcag ttcagaaggg ctgacacaaa tccgcgaaaa
     1201 agcacccgat cttgtaattc tggaccttat gatgcccgag atggacggat gggaagtttg
     1261 caaagctgtg cgtgccttta gcgatgtgcc gattatcgtg ctttcagctc tcaatgaccc
     1321 ctccatggtt gccagcgtgc tggatgcagg tgcagacgat tacctgacca agcccacacc
     1381 aagccgtgtg ctcgtggctc acatcaaacg tcttgtggat cgtaaggctt ccacagtgtc
     1441 tgtaaatcag aatttgccgg gaactttacc cgcctcctga taggcaaagc ccatctattc
     1501 tcaaggctgt ctccgttaaa aaaacatccc caccgcagag tggggatgtt gctttaggaa
     1561 agctgcttga gcgcttctgc ggtcttttcg gcggcttcca gcatcggaac atgaccgatt
     1621 ccccttaatt caacataatg tgaccccgga atggatgctt tgatctcacg cgcacgatcg
     1681 acagggataa gggcatcttc gtccccatgg atgaccacca ggggaaacgt aaacgaattc
     1741 agtgtcgcgg ttgtgtccgc acgttctgcc attgctttaa gcgcgccgat gtaggctgtt
     1801 ggctgttgct tttccataat tttgcgtgct gcatcctgta gtcgtggatc gccggtaaat
     1861 ttaggagcca tcgtttcgac caccccaccg atgccttttt cagcgacatc cgcagcagtc
     1921 ttatagcgac cttctttacg ctcaggtgga tcagctgggg tttgtgagga taccaaggca
     1981 agcccactta cgcgctctgg atacgttttc gcaaaagcca gagcaacata cccgcccatg
     2041 gaatgaccgg cgactgcagt cttttgaatg ccgagatgat ccagcaaacc agccaggtca
     2101 gatgcataat cgtccatcgt gtatggagtg tctaccgttt cagattcacc aaaccctcgt
     2161 aaatcaggga tgataagatc gaacgaattc tccagcaacg gaacaacctc gtcccataga
     2221 tgatgatcca gtggaaaccc atgcaagagt aggagtggag atccacttcc acgccgctca
     2281 taagccagat tgagattatt tacataaagc ttttccaaaa tttatctcct tcgtaattag
     2341 atatatcatt tccaatttac ctcaactttt aatttgcttc ccattcttga atagtagtgc
     2401 acaaatccat tttcactacc gaattcatgg attcggcgtg tgatatcgac atctgccttg
     2461 cagtactcag cgactttgtc cagttcgcca tttcgaaacc acctgaccga ttgtacgcca
     2521 tccccgctct ttgtagttcc caatgtcgct tttgctatgg agtcgagact caatcggaaa
     2581 tttaatgtcc gatagatatc tgccagcatg tcgatggtac gaatccctcg caaattcaca
     2641 tttggcgcat agggtctcaa gacctcataa tcgaagttaa taatattaaa cccgatcaca
     2701 cggtcggccg acttgagttc ctgtaccaat gccgccgcat cctgttccca atatacagca
     2761 aaatcttttc ttgctgtgga ccaggtgacg ccacaggcga gcaacaactt cgatggataa
     2821 cgcccaccca cttcatcgaa aagcttttga ctttccagat caaaatatac tgtattcatg
     2881 aagtttccaa attcttcaag tgttctcgcg caaactcgat ggcttgatca cgcgtttcga
     2941 tatttccaat tgcctggcct tcgcgaatct cttcgagcaa ttgtcccaca attcgccctg
     3001 gttcaaggtt taactcgcgc atcaaatcgt tgccatccag aagtcgaggc ggagaaatgg
     3061 tctgttcagg tctttcccaa tagttattca gcagcagctc agccacctgt aaagcggcag
     3121 tccatgcttc ctgtgataat gtatttccac gggttccacg cagatcggcg agtcctaata
     3181 acaaaagatc gatacctgct tcccctgtat cgcgaaagaa tcgataaatc gctcttcgtg
     3241 atggttcttg ttttagctcc tgcatctggt ttgtgaagaa atgaaatcgc atatggtttt
     3301 taacgatctt cttcactcgt tcgacctcat cattgctcag attgaaagac tgtgcacgtt
     3361 tcgaggtcac ctctgctcct tttatctcgt gctcgaagaa gcggatgcgt cccgtctcat
     3421 cgacggacct cgtggctggt ttctcaacgt catgatataa cgcggcgaaa aacaacaccg
     3481 cgcggaccga acggtctgtg ttgagcagtt tttcaaagtg ggcggtaaac ctttccttgt
     3541 attttccaag atgctgaatt agtaaagcac cgaaaggatc aatttcctct ggaggagcct
     3601 gttttatgcc taaggtcgtg atgatctctt ccaggtaccc caaaactttc aacgtatgtt
     3661 cccaaacgtc ataaacatga tgagaagatt gttctactcc tttcagggca gagagctctg
     3721 gcagaaggtg agggaagact ccgagcattt ccagggcacg gatggatgca tcaggtttgg
     3781 gtccttccag gatcttgaat aactcgtccc gtttgcgttc aggtgagatg tttggcagaa
     3841 gatgagcagc ctgcttcatg gatttacggg ttgcgagttc gatcttgaaa ctaaatgcag
     3901 cagcctgccg cacagcccgc aggatgcgaa ccggatcatc ggatatagaa gtctgtgagc
     3961 aagcacggat tagtttcagg cgcaaatctg cggtgccgtt aagtggatcg agcagcgttt
     4021 tcgaccgcac gtcatatgca atggcattga tggtaaagtc gcgagctctg aggtcttctt
     4081 ccagattggc gccccgatag gtggcgaaat caagaaaagt gcggtcacca tcagcttcag
     4141 atacgagcac tcgacctgta tcgcgttcct catcgagtgc cataaagtcc gctttaagtg
     4201 cgttggcaac gcgtcgggcg gtggagatgc cgttcgatgg caaagcaaag tcaaggtcat
     4261 gtgagaggcg gtttagcatc atatcccgca ctgcaccgcc taccaaatat atttcctgat
     4321 gtaggaggat ttcgctgacc ctgtccac
//
LOCUS       DASTJP010000611         9365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_683180, whole genome shotgun sequence.
ACCESSION   DASTJP010000611 DASTJP010000000
VERSION     DASTJP010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9365
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683180"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFQ23_17965"
     CDS             <1..249
                     /locus_tag="VFQ23_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908541.1"
                     /translation="MTIVVGILRAGGDTRYSLFLDGIIIWLVGVPMAYLGAFVFDLPV
                     YFVYLCAMSEEVTKWILGVARYRSRKWINNLAAQVEGI"
     gene            complement(262..1095)
                     /locus_tag="VFQ23_17970"
     CDS             complement(262..1095)
                     /locus_tag="VFQ23_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9908542.1"
                     /translation="MSDVKLFEISGDLSVVRQLPQQVDLDSITRQLPDGYYSTFRTYG
                     GCTRVLGLTSHLRRLYDYVSTPAASASALRHQLTRLLQSFCPDEARVRVVMTGQGQLY
                     VAMEPLKPLPMDVYEKGVRVETTEIRRNTPRMKSTAFISSSQGERAHLAQQGIFEALL
                     VKNGTILEGMTSNFFYVLRQDAASPYLRTSQRDILLGVTRRIVIRLARGRGVQVKFQP
                     LKLDQLTALSESFITSSSRGVVPVTQIDNVTVGQGSPGPITSTLRLAYEDYVLKHAEK
                     I"
     gene            1144..2502
                     /locus_tag="VFQ23_17975"
     CDS             1144..2502
                     /locus_tag="VFQ23_17975"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9908543.1"
                     /translation="MKQIFISYSRKDIDFARKLTNSFIDQELDSWIDWDDIPPSVDWM
                     MQIQKGIEETNVFLFLVSPDSLSSRVCRQEIEYAVKNGKRIIPIILRDIEMHQAYEPI
                     THLNWIFFRDADPYENSLEKLVTAIHTDYDWVQTHRRLQVKALEWERNHQEDSFLLRG
                     KDLQDAEAQLLVNGQKDPIATELQHQFISASRAAESVQIEAEQAREQQLRLEKSTGAR
                     MRRLSYVLLGIFTLAFFALFYWVYRSTTDLVLDAMRNELHAIIETGALSIDAQAFADL
                     LKLAPSTEGTSQDPLFISHREVLQNIQAANPNAQVYTVAASPNPEEVYIVANAYGILS
                     DLNDEFKYAYKVSDFNYDTLIGGMQETIVDMSIFTDQFGTWMSGCTPIKDAQENSLGA
                     LCADFEASFVYQAQSDATRTLKLAFLSIYPIVLILILSATQILQRVTSRFRKTGKEKA
                     KP"
     gene            2812..4659
                     /locus_tag="VFQ23_17980"
     CDS             2812..4659
                     /locus_tag="VFQ23_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908544.1"
                     /translation="MTTVQPTYEPTLTFDFRKAIHENRLKGLWRMMVDYRVPYMGATA
                     ALAVSALAKTFTYLLLQYFADDVLTQGRYIGNSLTQTFLWIGAAFIGLALVEGGFAFL
                     SGRLAAYTAEGITRRLRDFLFDHIQRLSFSYHATTPTGDLIERVTSDVDALRRFFSEQ
                     GIGMGRIILLFVINFVAILQINVKLGLVSVVVIPFILLVSLWFFKKVTKAYEEYQAQE
                     AILSTTLQENLTGVRVVKAFARQDYEKSKFEKDNWAKYLKGKILLYMHSMFWPLSDIV
                     LGFQMLFGFVFAANMAINDELTVGQYLAYVGLVVWLIWPIRNLGRIIVSASTGLVSYG
                     RLMEVVKQTREPLFDGKVQPTGPVRGEIRFENVSFMYSDGKSNALKNISFHVEPGQAI
                     ALLGSTGSGKSSLVNLLPRFHEYTGGRILLDGVELKDYSRSYLRKQIGIVEQEPFLFS
                     RSIRENITYGVGRDVSQEEVERAAKAAAVHDVILSFPDGYNTLVGEKGVTLSGGQKQR
                     VTIARTLLKNPRILILDDSTSSVDTETEAAIRDALNELMENRTTFIIAHRIQSVMIAD
                     LILVLDKGEIIQMGTHEELVSQLDGMYRRIYDIQTRIDEELELELAKAN"
     gene            4781..6643
                     /locus_tag="VFQ23_17985"
     CDS             4781..6643
                     /locus_tag="VFQ23_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908545.1"
                     /translation="MSDEMIEIEEEEYTSQLTAPVFKRIVGLLRPHWKWVLGFFVTIA
                     LTSATDAYFTYLNKDIVDQGIILGDTSALTRIAWIYGSIILFQAVTVFSFVYLAGVLG
                     ERVQYDLRQMLFNHLQELSLSYYAQNAVGRLIARVTSDTGRVADLLTWGVVDTTWAVM
                     NIITSVIFMALINWKLALIVFTVIPVMIFIAVQFRKRILVEYRTARRTNSKITGAYNE
                     NFQGVRVVKALMREDENTREFQQLTGTMYRSAYRAAWLSALFLPTVQIIAAIALGLIV
                     GYGGTQITLGVMTIGGIQAFVSYLTFMMWPVQDLARVYAEMQHSIASAERIFKLADTP
                     AEVHDKEDAIEATTLMGEIEFDQVNFYYEDRKPVLTDFSLKVNPGEMIALVGPTGGGK
                     STIVNLLCRFYEPREGVIRINGHDYMDYKLASIHSRIGIVLQTPHLFSGSVSDNIRYG
                     RLTASNEEIMDAAKIAGAHDFIVTLENGYDQNVGEGGNLLSVGQKQLISLARAVLARP
                     ELFIMDEATSSVDTLTEALIQRGMEALMKGRTSFVIAHRLSTIRRANRIVVIEDGKIA
                     EQGTHAELLRLGGHYYRLYTQQFRHELETHYGLAEAVGSEEKEKLPEPEAMAAD"
     gene            6744..7499
                     /locus_tag="VFQ23_17990"
     CDS             6744..7499
                     /locus_tag="VFQ23_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908546.1"
                     /translation="MNRKNLLVKYDKEMRLRMMYPEARKEITSDVVRFVRTAPGMNLV
                     GFTFAVEPELERVIDQQLDYFVPMRQPFTWKVYDHDHLPSLGEKLTKREFIREEDPRA
                     VMVFNLRNTPSQLSLSEEDDVRRISTQNELMDLIHVVDEVYSGDNSLLKQRLHSHMNV
                     PGYLSVYAAFEGNQPVSIAWTYFPYGQFAALSAGHQDERSYLSLLTARLSEIRERGYR
                     YAVLETSPTTQPNYEAHGFNQLTTVRNYQWTGN"
     gene            7896..8348
                     /locus_tag="VFQ23_17995"
     CDS             7896..8348
                     /locus_tag="VFQ23_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9908547.1"
                     /translation="MWVEIQVELSDFKMTQPDGNSYSNNKPVLIVEDNPVNRDLFSFQ
                     LRHYGVATRFATNGQEAVELMETEPDSFSMILMDLQMPVMDGITATQLIRQKEAATNR
                     RIVIIALTANESSGIQNQCIEAGMDDFVNKPSTLEKIGSILSKWIKEA"
     gene            complement(8357..9220)
                     /locus_tag="VFQ23_18000"
     CDS             complement(8357..9220)
                     /locus_tag="VFQ23_18000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9908548.1"
                     /translation="MTWIIGIALLVIVGFILDREIYFYEGVHLGPRVQSWLYDQWAKK
                     YDAGKHASQLRDPEMLAQPLLAALQNVTEPFVLDFATGTGRLSYALTSHPDFKGHIIA
                     LDLSQGMLEQAAAKLNRASSAHSASIRAPLSTKSVELLRHLALPLPFPDQSFDVVCAL
                     EVLELFPKMDQPLAEFSRVLRPGGIFLTSRGTEESGRRAKVKSKAEFEALLTKNDFTN
                     CQISPWWKLFDRVIAMKNGSLEPTGSRELSDVLHCTNCGQIQWQREDNVLSCGSCGRE
                     LAITKEGIVLN"
BASE COUNT         2407 a         2358 c         2311 g         2289 t
ORIGIN      
        1 atgaccattg tggtgggtat tctccgcgcg ggcggcgata cacgctactc cttatttttg
       61 gatggcatta ttatctggct ggttggcgtg cccatggcat acctgggcgc gtttgtgttt
      121 gacctgcccg tctactttgt gtatctgtgc gccatgtctg aagaagtaac taaatggatt
      181 ctgggagttg cgcgctaccg ttcgcgaaaa tggatcaata acctcgcagc acaggtggaa
      241 ggcatataag gtgacagtta cctaaatctt ttccgcatgt ttcaaaacat agtcttcgta
      301 agcaagtcgc agcgtggacg tgataggtcc cgggctcccc tgccccaccg tcacattatc
      361 aatttgcgtg accggaacga ctccgcgcga actggacgtg atgaacgatt cagacaacgc
      421 cgttagttga tctaatttca agggctgaaa cttcacctgg actcccctgc cccgcgccag
      481 gcggatgact atccgacgcg tgactccgag cagtatatcg cgttgcgatg ttcgcaagta
      541 cggtgaggcg gcatcctgcc ttaggacata aaagaaattg cttgtcatcc cttccaggat
      601 cgtcccgttt ttcaccaaca gtgcttcaaa aattccttgc tgggcaaggt gcgcacgttc
      661 accttgactt gaactgataa aggcagtcga tttcatacgc ggcgtgtttc ggcgaatttc
      721 ggttgtttcc acccgtacac ctttttcata tacatccatc ggcaatggtt tcagcggctc
      781 catggcgaca tagagttgtc cctgcccggt catcaccacc cgaacgcgag cctcgtctgg
      841 gcaaaaagac tgcaaaagcc tcgtcaactg gtgtcgcaga gcagatgcgc ttgcggcggg
      901 cgtggataca tagtcatata atcgtcgcag atgcgacgtt aacccaagca cgcgggtaca
      961 gccaccgtac gttcgaaagg tactgtaata tccatctggt aattgacggg tgatagaatc
     1021 cagatcaacc tgttgcggaa gttgccgcac aaccgacaga tctccagaaa tttcaaacag
     1081 ttttacatca gacatgatgc tgtcattgta ttccaaaaat gcttcacaac tcaggagtca
     1141 taaatgaagc agatctttat atcttattca cggaaagaca ttgacttcgc tcgtaaactt
     1201 acgaacagct tcatcgatca agagttagac tcatggatcg actgggacga tattccgccc
     1261 agtgttgact ggatgatgca aattcaaaaa ggcattgagg aaacaaatgt atttctcttt
     1321 cttgtcagcc ctgactctct ttcatcacga gtgtgccggc aagagatcga atatgcggtc
     1381 aaaaacggca aacggatcat tccgataatc ctgcgcgata ttgaaatgca ccaggcgtac
     1441 gagccgatca cgcacctgaa ctggatattt tttagagacg ccgatcctta tgaaaactca
     1501 ctggaaaaac ttgtaaccgc cattcacaca gattatgact gggtccagac acatcgccgc
     1561 ctgcaagtaa aagccctgga gtgggaacgc aatcaccaag aagacagttt tttgttgcgc
     1621 gggaaagatt tgcaggatgc tgaagctcaa ttgctggtga acgggcaaaa ggatccaatc
     1681 gccacagagc ttcaacatca gtttatcagc gcaagccgcg cggcggaaag tgtacaaatt
     1741 gaagccgaac aagctcgtga gcagcaactt cgcctcgaaa agagtacagg agcacggatg
     1801 cgacggctca gttacgtttt gctgggtata ttcactcttg cgtttttcgc tctattctat
     1861 tgggtataca ggagcaccac ggacctcgtc ctggacgcca tgaggaatga actacatgct
     1921 attatcgaaa caggtgcatt gagcattgac gcgcaggcct ttgccgatct gttgaagttg
     1981 gcgccttcaa cagagggtac aagtcaggat cccttattca tctctcacag agaggttttg
     2041 caaaacattc aggctgcgaa tccaaatgcg caggtgtata cagttgccgc cagccccaac
     2101 cccgaagaag tttacattgt ggcaaatgca tatggcatat taagcgatct aaatgatgaa
     2161 ttcaaatacg cttataaagt ttcggacttt aattacgata cgttaatagg agggatgcaa
     2221 gaaacaatcg tagatatgtc catcttcact gatcaatttg gcacgtggat gagtggctgt
     2281 actccaatta aagatgccca ggaaaattct ttgggtgcat tatgtgcaga tttcgaggcc
     2341 agctttgtat atcaggctca atcagatgca acaagaacac tcaaattggc tttcctgagt
     2401 atctatccaa ttgttctcat actcatctta tctgcgacac agattctcca aagggttacc
     2461 tctcgattta gaaaaacagg aaaagaaaag gcaaagcctt gacacttccg gctggcatgc
     2521 cgtaaaatcg accagatgca cgttaatctc aatgaaagga gcgcacaaat gaccaagttc
     2581 ttcctcggta gcctgatctt catcccagat tccgctggga ttttgccttg tcgtagtgcg
     2641 cctcctgacc atatataggt caatcctgtc cttgtttttt cgggctgtgg tgcactcacc
     2701 acagcctttt tatttgctac cctctcccaa tgggagaggg atagggtgag ggaaagactc
     2761 cgcccgcatc cactgccctt ctcccttagt gagaaggggg agagaaacga aatgactact
     2821 gtacaaccaa catacgaacc gacccttaca ttcgacttcc ggaaagccat tcatgagaac
     2881 cgcctcaagg gcctctggcg gatgatggtc gattatcgcg ttccgtacat gggtgcgacc
     2941 gctgccctgg cagtctccgc tcttgcgaaa acctttacct acttgctgtt gcagtatttt
     3001 gcagatgatg tgttgacaca gggaaggtac attgggaaca gcctgacaca gacgttcctg
     3061 tggatcggag ccgcttttat agggctagcc ctagtcgaag gtggattcgc atttctgtcc
     3121 ggacgtctgg ctgcctacac agccgaggga atcacgcgcc gcctgcgcga cttcctgttc
     3181 gatcatattc aacgactgag cttctcgtat catgctacaa cgcccacggg cgacctgatc
     3241 gaacgcgtca cttccgatgt ggatgctctg cgccgctttt tcagcgagca aggcatcggc
     3301 atgggtcgca tcatcctgtt gtttgtgatt aactttgtgg caatcctgca gattaacgtc
     3361 aaactgggtt tagtctcggt ggtcgtcatc ccgttcatcc tgctcgtgtc gctctggttc
     3421 ttcaaaaaag tgacaaaagc ctatgaagaa tatcaggcgc aggaagccat actctccacc
     3481 accctgcagg agaatctcac tggcgtacgc gtggtcaaag cctttgccag gcaggactat
     3541 gaaaagagca agtttgaaaa agacaactgg gcaaaatacc tgaaaggcaa gatcctgctt
     3601 tacatgcatt cgatgttctg gccgctctca gatattgtgc ttggttttca gatgctattt
     3661 gggtttgtgt ttgcggcaaa tatggcgatc aatgatgagc tcacagttgg tcagtactta
     3721 gcctatgttg gtcttgtcgt ctggttgatc tggccgattc gcaacctcgg gcggatcatc
     3781 gtctcggctt caacagggct ggtgtcctat ggccgtctca tggaagtcgt caagcagacg
     3841 cgcgaaccgc tattcgatgg caaagtccag cccaccggtc cggtgcgagg cgagatacgc
     3901 ttcgaaaatg tctcgttcat gtattcggat ggcaagtcga acgccctgaa gaatatttcc
     3961 ttccacgtgg agcccgggca ggctatagcc ctgcttggct caactggctc aggcaagtcc
     4021 tcgctggtca acctgctgcc acggttccat gaatatacgg gcggaaggat tctgctggat
     4081 ggcgtggaac tcaaagatta ctcacgctcg tatctgcgca agcagattgg catcgtggaa
     4141 caggaaccgt tcttgttcag ccgctcgatt cgggagaaca tcacctacgg cgtgggtcgg
     4201 gatgtatcgc aggaagaagt agagcgagca gccaaggctg ctgcggtcca tgacgtgatc
     4261 ctgtcgtttc cggacggcta caacacgctg gtcggtgaga aaggcgtgac tctttcgggc
     4321 gggcaaaagc agcgtgtgac cattgcacga accctgctca agaaccctcg cattctgatc
     4381 cttgatgact caacctcatc ggtggatacg gaaaccgaag cagccattcg agatgccttg
     4441 aatgagttga tggaaaatcg aacaaccttc atcattgcgc accgcattca gtccgtgatg
     4501 atcgcagact tgatcctggt gctggacaaa ggcgagatta ttcagatggg cacgcatgaa
     4561 gaacttgtgt ctcagctgga tggcatgtat cgtagaattt acgacatcca gactcgcatc
     4621 gatgaagagt tggaactgga gctcgcgaaa gcgaattaat ggaacaacct gtcattgcga
     4681 ggagccgctg agcgacgacg aagcaatctc gagtttgaag gttagctgag attgccactc
     4741 ctacttcgta ggctcgcaat gacatatcta agaggcaaaa atgtctgacg aaatgattga
     4801 aatcgaagaa gaagaatata catcacaatt aaccgcgccg gtgttcaagc gcatcgtggg
     4861 cttgctccgg ccgcactgga aatgggtgct gggctttttc gtcaccatcg cgctcacttc
     4921 cgctacggat gcctacttca cctatctgaa caaggacatc gtggaccaag gcatcatact
     4981 gggtgatacc tccgcactta cgcgcatcgc atggatctac ggctcgatca tccttttcca
     5041 ggctgtgacc gtgttctcat tcgtttatct ggcgggtgtg ctgggtgagc gcgttcaata
     5101 cgatcttcgg cagatgctct ttaatcatct gcaggagctt tcgctatcct actatgctca
     5161 gaacgccgta ggacgcttga tcgcccgcgt cacgtctgat accggccgag tcgctgacct
     5221 attgacctgg ggtgtcgtag ataccacctg ggcagtgatg aacattatca cctcggtgat
     5281 cttcatggcg ctcatcaact ggaagctggc cttgatcgtc tttacggtca tccctgtgat
     5341 gatttttatt gccgtgcagt tccgcaagcg gattctcgtg gagtatcgca ccgcccgccg
     5401 gaccaattca aagatcaccg gcgcctacaa cgaaaacttt cagggtgtgc gcgtggtcaa
     5461 ggcgttgatg cgtgaggacg aaaataccag agagttccag caacttactg gaacgatgta
     5521 tcgatccgcc tatcgggctg cgtggctttc tgccctcttc ctccccaccg tgcaaatcat
     5581 cgccgcgatt gcgcttggtc tcattgtcgg ttacggtggt acgcagatca cactgggtgt
     5641 gatgaccatt ggcggcattc aggcttttgt ttcctatctc acattcatga tgtggccggt
     5701 tcaggatctg gcccgcgtct atgccgaaat gcagcattcc atcgcctctg cggagcgaat
     5761 cttcaaactc gcggatacgc cagctgaagt gcacgacaaa gaagatgcga tcgaggcgac
     5821 cacgctgatg ggcgagatcg aatttgatca ggtaaatttc tactatgagg atcgtaagcc
     5881 ggtgcttacc gatttttcgc taaaggtcaa ccccggcgaa atgatcgccc tggtcgggcc
     5941 gacgggtggt ggcaaatcca cgatcgtgaa ccttctctgt cgcttctatg agccacgcga
     6001 aggcgtgatc cgtatcaacg gacacgatta tatggactac aaattggctt cgatccatag
     6061 tcgtattggc atcgtgcttc agacgcccca cctcttttca ggaagtgtga gcgacaacat
     6121 ccgctacgga cggctgaccg cctccaacga agagatcatg gatgccgcaa agatcgcggg
     6181 cgcacatgac tttatcgtca cgctcgaaaa cggctatgac cagaatgtag gcgaaggcgg
     6241 caatctcttg tctgtcggtc agaagcagct gatcagcctg gcgcgtgcag ttttagcaag
     6301 accagagttg ttcattatgg atgaggcgac ttcatcggtc gatacattga cggaggcctt
     6361 gatccagcgg ggcatggaag ccctgatgaa aggccgaacg tcattcgtga tcgcacatcg
     6421 cctgtcaaca attcggcgtg caaatcgaat cgtcgtcatc gaagatggca agatcgccga
     6481 acaaggaacg catgctgagc ttttgcgcct tggtggacac tactatcgtc tctacaccca
     6541 gcaattccgc catgaattag aaacccacta tggcttggcg gaagccgttg gaagtgaaga
     6601 aaaagagaag ctgcccgagc cggaggcaat ggcagccgat taaaccaccc cctccgtccc
     6661 tgccggggca cctcccccaa atccgacgaa cgtcagtcgg gtttggggga ggctgggtgg
     6721 ggtagaccgt aaaggtaaat atcatgaaca gaaaaaactt attagtcaag tatgacaaag
     6781 aaatgcgttt gcgcatgatg tacccggaag cgcgcaagga aattacaagc gacgtagttc
     6841 ggtttgtgcg cacggcccca ggcatgaatc ttgttgggtt cactttcgct gttgaacctg
     6901 aattggagcg cgtgattgac cagcagctag attacttcgt tcccatgcgg cagcccttca
     6961 catggaaggt ctacgaccac gatcatctgc ccagcctggg tgagaaattg acaaagcgcg
     7021 aatttatccg tgaagaggac cctcgggccg tgatggtctt caacctgagg aacaccccgt
     7081 cacaattgtc cttatcggaa gaagatgatg tgcgacgtat ctccactcag aacgaattaa
     7141 tggacctcat tcatgtagtg gatgaagtct attctggcga caattctttg ctcaagcaga
     7201 gactacattc gcatatgaac gtccccggtt atttgagtgt ctatgctgct tttgaaggga
     7261 atcaaccggt atcgatcgcc tggacatatt tcccgtatgg tcagttcgct gccttatccg
     7321 cggggcatca agacgaacga tcctatctta gccttcttac agccagatta agcgagattc
     7381 gcgaacgcgg ctacagatat gcagttcttg agacaagccc caccactcaa ccaaattacg
     7441 aagcgcatgg cttcaatcag ctgacgaccg tgcggaatta ccaatggaca gggaactaaa
     7501 aacataaaaa aagccgacca tttaatggtc ggctttttag aatgaataga agtattcggg
     7561 ccaggaaaat cagtcctttc gtactttgac tctgctcttg gcgggtgaat atggtctgaa
     7621 atattccacc gtatacccag tgacaagcca ggccaaactt ccgagtgcga caccaacgaa
     7681 aaattttagt aatgtaagca tttcaaactc cataatgtaa taactgcgtc atcaacagtt
     7741 tcgatgcctt tgtgctaaca tgacgtggat tttgcaaaac gttacggaga tgaccttaca
     7801 aaaagataca ggtcgcaaaa agcgaccagc aaagaacggt caccaaaagg gctgcaactt
     7861 tttaccggat ttcagaagta ggagcgtaca gccgtttgtg ggttgaaatt caggtagaat
     7921 tatctgactt caaaatgacc cagcccgacg gcaatagtta ttccaataat aaaccggtgt
     7981 taatcgttga ggacaaccct gtcaatcgcg atttgttttc gtttcagctt cgacactacg
     8041 gggtggcaac acggtttgcc accaacggcc aggaagccgt tgaattgatg gaaacggaac
     8101 ccgattcatt ttccatgatc ctgatggatc ttcaaatgcc agtgatggac ggcatcactg
     8161 cgacacaatt gatacgtcaa aaggaagctg ccaccaaccg acgcatcgtt attattgcgc
     8221 tgaccgccaa tgaatcatca ggtattcaaa atcaatgcat cgaggctggc atggacgact
     8281 ttgtcaataa acccagtacg cttgagaaga tcggcagtat tctcagtaag tggatcaaag
     8341 aagcctgaag aaaaaattaa ttcagtacga tcccctcttt tgtaatcgcc aattccctac
     8401 cacaacttcc acaacttaag acattatctt cccgctgcca ttggatctga ccacaatttg
     8461 tgcaatgcag tacgtcactc agctctcgtg aaccggtagg ctccaaagag ccgtttttca
     8521 tggcgatcac acggtcaaat aacttccacc agggtgagat ttggcaattc gtaaaatcat
     8581 ttttcgttaa taacgcttca aattcagctt tgctcttgac cttcgctcga cgaccagatt
     8641 cctcagttcc ccgagatgtt aaaaatattc cgcctggtcg aagcacacga ctgaactcag
     8701 caagcggctg gtccattttc gggaaaagct ccaacacctc aagcgcgcat actacatcaa
     8761 aagactgatc aggaaatgga agtggcaaag ccagatgccg aagcaactcc acacttttcg
     8821 tgctgagcgg agcacgaatg gaagcggagt gagcagacga agcgcggttt aatttcgcag
     8881 cagcttgttc cagcattccc tgcgaaagat cgagtgcaat aatatgtcct ttgaaatccg
     8941 ggtgactggt caacgcataa gataggcgcc cggtacctgt tgcaaaatcc agaacaaatg
     9001 gttcagtaac attttgcaag gcagctaaca aaggctgagc aagcatctcc ggatcgcgca
     9061 attggcttgc gtgctttccc gcgtcatact tcttcgccca ctgatcatac aaccatgact
     9121 gcacacgcgg tccgagatga acgccttcat agaaatagat ttcgcggtcg aggataaaac
     9181 cgacaatcac aagtagtgca atcccaataa tccatgtcat agcgagacca aatcacctta
     9241 gccagtctgt attacctgcg ctggcgaatt gcttcgtata ctatcaaagc cgtagagacc
     9301 gaaacattta acgaattgac ctttcccatc attgggattt ttactctcat atctgcctga
     9361 cttaa
//
LOCUS       DASTJP010000612         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_577739, whole genome shotgun sequence.
ACCESSION   DASTJP010000612 DASTJP010000000
VERSION     DASTJP010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577739"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFQ23_18005"
     CDS             complement(<1..379)
                     /locus_tag="VFQ23_18005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908549.1"
                     /translation="MIHILIADADPAARKALSLLLGRKLSIKEVREVGDVEALIRARA
                     DGPPELLLLDWKLYGSPPLETCRLLQKAFPHLKIILLSLDANDESIARNAGAGFIHKG
                     AAPDTILNRLMSVINQESLSCSIS"
     gene            complement(486..1112)
                     /locus_tag="VFQ23_18010"
     CDS             complement(486..1112)
                     /locus_tag="VFQ23_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908550.1"
                     /translation="MTIKILLVDDHAVVRSGLSKFILINKDMKMVGEASDGEEAVQKA
                     SLYKPDVILMDLMMPGMDGITATHEIRQKYPKIKIIVLTSFAEQNMIQGALQAGAIGY
                     LQKNVTAKELVQAIRMACEGKMTLSPEAMQVLAYAVPQPQIRAGRLTERERDVLKCMS
                     DGLNNHEIAERLVVSIGTVKFHISNIFQKLGVDSRVEAVKLAIEQKLA"
     gene            complement(1143..2441)
                     /locus_tag="VFQ23_18015"
     CDS             complement(1143..2441)
                     /locus_tag="VFQ23_18015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9908551.1"
                     /translation="MMKDQSGEIHEEQLSAFTELSQLLEIQVTQQASGESKIAQRAIN
                     ALKESDARFHAVFENSAVGIVLMSLERKPIAINAVTEKLIGYSFSEVEDIDPRTLAVP
                     EDRSMDDGLFKDLVEGKRESYVMERRYRRKDGRIIWARINYSLVRAIDGKPDYLIGII
                     EDIDEQKRAAERISEQKAIDLALLQQRVEERTHELAETNQRLKQEIDQRKRVEDELAK
                     KAAEEAVTADRTRLARDLHDAVTQTLFSASLTAEVLPDLWEVDVEEAKKSTEELRQLT
                     RGSLAEMRTLLLELRPSTLIQTSLNDLIKQLCEAFIGRSRLPINMKIEGNATLPPEVQ
                     VAFYRIAQESLNNAFKYARATAVNVKLYVSSSGVHFETCDNGIGFDVTRIKPMTLGMR
                     IMRERAEAIGANLQITSKPGSGTCVAMTWREDPNFRLRVL"
BASE COUNT          627 a          745 c          672 g          793 t
ORIGIN      
        1 atgagattga acaacttaac gattcctgat tgatcaccga catcagcctg ttcaggatcg
       61 tatctggcgc cgcgcccttg tgaatgaaac ctgcaccggc gttcctggcg atcgattcgt
      121 catttgcatc caggctgagc aggatgatct tcaagtgagg aaacgccttt tgaagcaggc
      181 ggcaagtttc gagtggtgga gagccgtaca atttccaatc gagcaggagg agttccggtg
      241 ggccatctgc ccgcgcgcga atcaaggctt ctacatcccc aacttcccga acctctttta
      301 tgctgagttt cctgccaagc aacagactaa gtgctttacg cgccgcgggg tcggcatcgg
      361 cgatcagaat atgaatcatg agacctcgtt tgtagtctat gccaggcgag gtctctgtgt
      421 atcagacgat ggtggagtct ttagctagcc agatggatag tgtgtttcat tcgcccccgg
      481 attcgtcaag ccagcttctg ttcgatcgcc aacttgacag cttccactcg gctatccaca
      541 ccgagcttct ggaatatgtt agagatatgg aacttgacgg tcccaatgct cacgaccagc
      601 ctttcggcga tctcatggtt gttgagccca tcggacatgc atttcaatac atcccgctca
      661 cgttcggtca atcgccctgc gcggatttgt ggctgcggca cagcataggc taacacctgc
      721 atcgcctcgg gcgaaagagt catttttcct tcgcaagcca tgcggattgc ctgcaccagt
      781 tccttggcag tgacattttt ttgcaggtag ccgatcgcgc cggcctgtaa ggcaccctgt
      841 atcatgtttt gttcagcaaa acttgtcagc acgatgattt tgatctttgg atatttttga
      901 cgaatttcat gggtggcggt gatgccatcc atacctggca tcatcaaatc catcagaata
      961 acatcgggct tgtacagtga tgccttttgc acggcctctt caccatctga agcttcgccc
     1021 accatcttca tatctttgtt gatcaggatg aattttgaca gtccacttcg caccactgca
     1081 tggtcgtcca cgagcaaaat tttgattgtc attttctcct ctttttcggc acggtccgcg
     1141 aattacaaga ctcgcaatct gaaatttggg tcttcgcgcc aggtcattgc cacgcatgta
     1201 ccagagccag gtttgctggt gatctgtaga ttggctccga ttgcttcggc tctttcacgc
     1261 atgatgcgca ttcccagagt catcggcttg atcctggtca cgtcaaagcc aatgccgtta
     1321 tcgcacgtct cgaaatgcac gccagaggac gaaacataca gcttgacgtt gacggctgta
     1381 gcacgcgcat acttaaaagc attgttcaag ctttcctgtg caattcggta gaacgcgacc
     1441 tgtacttccg gtggcaacgt ggcatttcct tcgatcttca tgttgatagg caggcgagaa
     1501 cgcccgatga atgcctcaca tagctgcttg ataagatcgt tcagggaagt ttgtatgagc
     1561 gtggatggac gtaattcaag gagcagggtt ctcatttctg ccagggaccc gcgggttaat
     1621 tgacgcagtt cctccgtaga tttctttgcc tcctcaacat ccacttccca caaatccggg
     1681 aggacttctg ccgtcaggct ggctgagaac agagtttgtg tcacagcgtc gtgcagatca
     1741 cgagccaggc gggtacggtc tgctgtaacg gcttcttctg ccgccttttt agccaattca
     1801 tcttcgaccc ttttgcgttg gtcgatttcc tgtttcaatc tttgattagt ttcggcaagc
     1861 tcgtgagtac gctcttcaac gcgttgctgc agcagtgcaa ggtcgattgc cttctgctct
     1921 gaaatacgtt cggctgcacg cttttgttcg tcaatgtctt ctatgatccc aatgagataa
     1981 tccggcttgc catcgatagc acgaacaaga gaataattta tgcgagccca tatgatccgt
     2041 ccgtctttac ggcggtaacg ccgttccatc acataggact cgcgttttcc ttccaccaga
     2101 tctttgaaca acccatcgtc catcgacctg tcttctggga ctgccagagt gcgcggatcg
     2161 atatcttcaa cctcgctaaa ggaatagcca atgagcttct ctgtaactgc attgatcgca
     2221 atgggtttgc gctccagact catgagcacg attcccactg cactattttc aaatacggca
     2281 tggaaacgag cgtcagactc tttcaaggca ttgatggcgc gttgagcgat cttactttcg
     2341 ccacttgcct gttgtgttac ctgtatttca agcaactgac ttagctcagt gaaggcggag
     2401 agctgctcct catggatttc gccagattga tctttcatca ttgttatttt ctatctaaag
     2461 aatatcttgc attgaaagct catcttccaa cggttcgaga tgtgtaaata cgctcgccga
     2521 accacctaat accttccgaa tacttgactc gaaatcctct gcgacatgat gcgcatcctg
     2581 aacagtccaa ttaattggcc cgttgtatga ggtcgctcgt tccttcaaat gcttctgatg
     2641 gggcctgtat acattatcgt tgctctaacc tggatcgtca tgggtcatcc gtccccaaaa
     2701 aagcaggaca cttgcctcta gacctcgtgt ggaggactag agataaaagg cggtagtgcg
     2761 ttcatacgga aattgtttta ttcttcatta tctccccctg ggtgtccctg acagtacaaa
     2821 gcagggtatt cgcacga
//
LOCUS       DASTJP010000613         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1211431, whole genome shotgun sequence.
ACCESSION   DASTJP010000613 DASTJP010000000
VERSION     DASTJP010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211431"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="VFQ23_18020"
     CDS             complement(<1..1499)
                     /locus_tag="VFQ23_18020"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9908552.1"
                     /translation="MSKSKFLLVSLVMILLAVACAPVLTPASPTLPPILTEEPAVPAI
                     PITGVAVVQSLDVQILETSPLSVNLIVRGQLPDAGCTTISGVNQTRDGNTIKVNLTTT
                     TDPVALCAQSLTPFEQVFPLDVSNLPPARYVVNVNGLEQTFELVTRDATKFKQDLVAA
                     LNARNYELMKVMMGESFMIGYWLSEGTTNTPDAAVEQLRTNLLNSSSPVTADYGRNLV
                     ELLGTDPVTIVGPNVVEASPLLVSGLGTEGKDQAILFNAKLPDGSLYWHGMLFAKDGF
                     VKPAPVVTPVDTNAYPTDVKFVMAQQDVRLRSGPGTQFRILGFVAEGQTAKVTGVSAD
                     RNWWRVICPDNSTGSCWVSAARNLTQPTDGPSPLPDTTAYPTDVKYVLAQQNVNIYSG
                     PGTQFSILRSIFAGQTAQVTGVNVDGKWWRVICPDSSLGSCWVSADTSLTKPTGLSSN
                     ADVQTVEIQILESYPLQVNAIARGYLPDAGCTTISSVNQSREGNVFKVT"
     gene            1669..2298
                     /locus_tag="VFQ23_18025"
     CDS             1669..2298
                     /locus_tag="VFQ23_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9908553.1"
                     /translation="MNAGQDYLLIVEDDRDILRLLDAALTFKGYRVILSHNGKEALEI
                     IQLKRPQIIITDIMMPQLDGFGLVHRLRIHPDTCNIPVVFITATFVAPEDRDFALNIG
                     ATRFIQKPVDLEKFLSTIEELLKQDAPPAIEPFKELYFYEGYRRRLETKLDQKMKQIA
                     REEHLLETPSKAEDPHLQGLLRRSIRERDEIRFLLFQVYEQIEKYNRTN"
     gene            complement(2295..2894)
                     /locus_tag="VFQ23_18030"
     CDS             complement(2295..2894)
                     /locus_tag="VFQ23_18030"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9908554.1"
                     /translation="MKPKETGLHYDQLAQSWQQRHQHSAYGLAALERAIRFVDNKSTA
                     LDIGCGSSGRFIEVFIKNGFTPTGLDISAEMIALAKQLHPEVTFYADDICTWQFPQNY
                     DLITAWDSTFHLPLSEQKPVLKKMCDGLNPNGVLLFTCGGTTGPGEISGGFDGQTFDY
                     STLGVNEFLRLVMEFGCACKHVEYDQFPENHVCVIAQKI"
     gene            3386..3721
                     /locus_tag="VFQ23_18035"
     CDS             3386..3721
                     /locus_tag="VFQ23_18035"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET9908555.1"
                     /translation="MSTAGKLRKDSTPAERKLWSKIRNDRLGVTFRRQHAIGKYIPDF
                     ASPRAKLIIELDGSQHLEQQEYDQERTKYFESLGYKVIRFWNSDVMNRIDEVLLSISY
                     TIEENSTSE"
BASE COUNT          964 a          930 c         1005 g          946 t
ORIGIN      
        1 atcgtcacct tgaaaacgtt gccttcgcga ctctgattga cggacgaaat cgtcgtgcag
       61 ccggcatcgg gcaggtatcc gcgtgcgatg gcgttgacct gcagcgggta ggattccagg
      121 atttgaatct ccacggtttg cacatcggcg ttgctgctca aaccggtcgg tttggtcagg
      181 cttgtatcgg cggagaccca gcagcttcca aggctgctat cggggcagat cacgcgccac
      241 cattttccgt ccacattgac tccggttacc tgagcggtct gcccggcaaa gatggatctc
      301 aggatgctga actgtgtacc gggtccgcta tagatgttca cgttttgttg agcaagcaca
      361 tacttaacat cggttgggta ggccgttgta tcaggaagtg gtgaaggtcc atcggtcggt
      421 tgggtaaggt ttcgggccgc cgagacccag cagcttccgg tgctgttatc cgggcagatc
      481 acgcgccacc aattgcggtc ggcgctcaca ccggttactt ttgcggtttg tccctctgcg
      541 acgaacccaa ggattcggaa ttgtgtgcca ggtccactac gaaggcgaac atcctgctgt
      601 gccatcacga acttgacgtc ggtcggataa gcgttggtat ccacaggtgt aaccaccggt
      661 gcaggcttga caaatccatc cttggcgaag agcatgccat gccagtacag gctgccatcg
      721 ggcaatttgg cattgaaaag gatcgcctgg tctttacctt ctgtgcccag ccctgagacc
      781 aaaagcggac tggcttcgac cacattcggt cccacgattg tcactgggtc agtgccaagg
      841 agttcgacca gattccgacc gtaatcggcg gtgacgggcg aagaggaatt gagcaggttc
      901 gtcctgagct gttcaacagc agcatccggg gtgttggtcg ttccttctga aagccagtag
      961 ccgatcataa aggattcacc catcatcact ttcatcagct catagttgcg tgcattcaac
     1021 gcggcaacta agtcctgttt gaatttcgtg gcatcgcgtg taaccagttc aaaggtttgc
     1081 tccaggccgt tgacgttgac cacataccgg gcgggtggga ggttgctgac atccagaggg
     1141 aaaacctgct cgaacggtgt aagcgactgc gcgcagaggg cgacggggtc tgtagtggtc
     1201 gtgagattta ccttgatcgt gttgccgtcg cgcgtctgat tgactcctga tatcgtcgtg
     1261 catccggcat ccggcaattg cccacggaca atgagattga ctgagagcgg agatgtctcc
     1321 aaaatctgca catccaggct ttgtacgacg gctacgcccg taattgggat ggctggtacg
     1381 gccggttcct cagtaagaat gggtggcaga gtcggcgagg ccggcgtgag aactggggca
     1441 catgcaaccg ccagcaaaat catgaccagt gagacgagta agaactttga tttcgacatg
     1501 agtagctcct ttctgttatt gaacgtggca atgacgatgt tatacagaaa aaggttccta
     1561 ctcgggtaca aaatagtcat acaaaaatat gtacaaaaaa ctacactgtg taacaaaaag
     1621 tgatgaaatt aatataactg gcgcggtggg gcgatgtaga ataagaggat gaacgccgga
     1681 caagattatc tattgattgt ggaagacgac cgcgatattt tgagattgct ggatgctgcc
     1741 ctcacattca agggatatcg tgtcatcctg tcccataacg gcaaggaagc cctggaaatc
     1801 atccagctca aacgccctca gataattatt accgacatta tgatgcctca actggatggt
     1861 ttcgggctgg tccatcgact gcgaatccac ccggatacct gcaatattcc cgtggttttt
     1921 atcactgcta catttgtggc gccggaagat cgggatttcg cgctgaacat cggtgcaacc
     1981 cgctttatcc agaagccggt ggatctggag aagttccttt cgaccattga agaacttcta
     2041 aagcaggatg caccccctgc catcgaaccg ttcaaggaac tgtattttta cgaggggtat
     2101 cgaaggcggc tggaaaccaa gctggaccaa aagatgaagc aaattgcccg tgaagagcat
     2161 ctgcttgaaa cgccgtcaaa agcggaggat ccacacctgc agggattgct tcgacgatca
     2221 attcgtgaac gcgacgaaat aaggtttttg cttttccagg tctacgaaca gattgagaaa
     2281 tacaatcgga cgaattagat cttctgcgca atgacgcaaa cgtgattttc ggggaactga
     2341 tcgtattcaa cgtgtttaca ggcacaacca aattccatga cgagccgcag aaattcattc
     2401 acgcccagcg tactgtagtc aaaggtttga ccgtcaaacc cacctgagat ttcgcccggt
     2461 ccggtcgttc ccccgcacgt gaataagagc acgccgttgg ggtttaatcc atcacacatc
     2521 ttcttcagca ctggtttctg ttcggaaagg ggcaaatgaa aggtactatc ccaggcagtg
     2581 atcaaatcat agttctgagg gaattgccac gtgcagatgt catcggcata aaatgtcact
     2641 tccggatgga gctgcttcgc aagggcgatc atttcagcag aaatatccag acccgttggc
     2701 gtaaaaccat tcttgatgaa gacctcgatg aagcggccgc tgctgccaca tccgatatcc
     2761 agcgccgtgg acttgttatc cacgaatcga atggcccttt cgagagcggc gagaccatac
     2821 gcagagtgtt gatgtcgctg ttgccacgat tgggcaagtt gatcgtaatg cagacccgtt
     2881 tcttttggtt tcatcttgat gtaagacaag tatagcgtat gagagaattt gctgtttgcg
     2941 atatcttgac ttgaacttgt atgtttaaca tttttcctgt atgctttatt tatatcgctg
     3001 gatcgttcat ttcaatcgag attctgcctc actgattatt ctccacgcta aaaatggagt
     3061 ccccagctgg cagaattggt atgactagga gcagtccgag gcggggatgc tccgcgattt
     3121 gagtcattgt tgaatgagag ttttgggtaa ttgccccatc ccgtcctcac cgctcgcttc
     3181 gctcgggtgc gcttcgcagt cccccaaatg cggaagagcg tcgaatttgg ggaaggtgaa
     3241 tgtgaagttg gaatgaatat atttattatc gcccaagtta agattgtcct tcccccattt
     3301 tcgtcccgct gctttttgcg ggatgcaaat gggggaacac aaagggggca aaatgccatg
     3361 ccccaacaga agtaacccaa aaacaatgag cacagctggt aagcttcgaa aggattctac
     3421 tcctgccgaa cgcaaactct ggtctaaaat cagaaacgac cggttgggag tcacatttcg
     3481 tcgtcaacat gctattggca aatacatccc cgacttcgct tcacccaggg caaagctcat
     3541 cattgagctc gatggcagcc aacatctgga acagcaagag tatgaccagg agagaacaaa
     3601 atattttgaa tctctaggct ataaagtcat tcgcttttgg aacagtgatg tgatgaatag
     3661 gattgatgaa gttctgcttt ctatttcgta tacgattgaa gaaaattcca ctagtgaata
     3721 agtccagctt tatcagcaca caaaccccgg atctattgtt ggattatccc aaaaagcaag
     3781 cgccatttgc atagcacaga gcgaagaacg cgttacccaa tgtccgtttg cggggaattc
     3841 gtgga
//
LOCUS       DASTJP010000614         7510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1305868, whole genome shotgun sequence.
ACCESSION   DASTJP010000614 DASTJP010000000
VERSION     DASTJP010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305868"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFQ23_18040"
     CDS             <1..273
                     /locus_tag="VFQ23_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HET9908556.1"
                     /translation="QLIEYCKQNNQSLLVTSATAEKTVDEIANIPKRLLIVFGSEKTG
                     SSPEIEQAATLRVRIPINEKVESLNVSAAAGITLFNRIQFNRSKSQ"
     gene            complement(304..1089)
                     /locus_tag="VFQ23_18045"
     CDS             complement(304..1089)
                     /locus_tag="VFQ23_18045"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9908557.1"
                     /translation="MQTLKLLSAFLKVNVQMSLAYRADTVVNILLNLMWLGWELLSLN
                     IIFSNTETIGGWGFGELVALLGVFRLVHTLMIALIWPNTEKFNQSIRDGSMDYTLLQP
                     INSMFLVTFSRITVWRAWDLILAIVLISIGINISGDTITALNILTFILLTVSGSIVIY
                     SLWIVLIALTFWFTKFDNNVTILQALLDAGRYPVTVYPVWLRIIVTFVIPVAVATTVP
                     LQALRGDLNAGRVLMYLLIGIASFLIASQVWKAGLKRYSGASS"
     gene            complement(1092..1889)
                     /locus_tag="VFQ23_18050"
     CDS             complement(1092..1889)
                     /locus_tag="VFQ23_18050"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9908558.1"
                     /translation="MLTIFQRLWQVNWAEQWQYRANLIMYLLYWLVSPIIYLAVWTSI
                     ASTKGDVNGFTANDFVTYYMTLLIVDQITSNIVIHTFAYKIQDGTLSGELIKPIHPML
                     TNALVNNISFKGLTIMGLAPIWIILFFLFRPDFSHVTLTGILLAIPAMIMGFFIGYLL
                     SAVITALAFWTTRVYSIHEFYYALILLFSGQFVPLTLMPKVIQEIAQYLPFQMLIYFP
                     VQLILGNLSSAQIIQGYIVGFIWLILSITLFNWVWRNGVKRFSAVGA"
     gene            complement(1916..2896)
                     /locus_tag="VFQ23_18055"
     CDS             complement(1916..2896)
                     /locus_tag="VFQ23_18055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9908559.1"
                     /translation="MSPITINAENLTKTYQVPEREGGFGAAVRSFFKRKYKDVKAVQQ
                     VNFEIAQGEVVGFLGPNGAGKTTTLKMLSGLLHPTDGNANVLGFTPWELKPEYLRSMT
                     LVMGQRNRLSWDIPAADSFLLNQAIYRIPDEQYQATYKELDELLELEPLMKKPVRNLS
                     LGERMKCEIAAGLLHRPKVLFLDEPTIGLDITGQSRIREFLREYNKRTGATILLTSHY
                     MADVTALCERIIIIHHGKLKYDGGIGDLSRKIAPFKLIGVRLAEVDSHDLSKYGTPVQ
                     NEEDWEKQYIQVKAEDVTRVTSGLLSDLPIHDITITDPPIEDVIEQAFNQ"
     gene            complement(3070..3861)
                     /locus_tag="VFQ23_18060"
     CDS             complement(3070..3861)
                     /locus_tag="VFQ23_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908560.1"
                     /translation="MKPKSLRSVLWLASLLMLVSSACGTSAAPPTSTSTPDPTATATI
                     TLTPTNTPRPTVTPRPTRTPNLAATQQMEEVNGQTQGYFDLGYLSTTDGEVTHHEDSS
                     EEWAQLDWYRPSTILTVARDFYVSAHFKWTSAYRNAGDSGCGFVFAANENGDHYAVFL
                     DRAKIIFLDNVSSSSYSRSVGLTRGTGVVKFGNPFDEPVEADFTLIVEDAYARVLIDD
                     EVVGEYTLSQSRALDGFIGLALLSGTNRDFGTRCEVTNMHTWVSQ"
     gene            4061..4933
                     /locus_tag="VFQ23_18065"
     CDS             4061..4933
                     /locus_tag="VFQ23_18065"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET9908561.1"
                     /translation="MQIAKEIREKILVFQRLEITEHHIYKKLAGKIQSPENAKIVAQI
                     ADDELRHYEGLKKYSGQDVSPDRLTLWTYYIISRIFGFTFGIKLLERSEKKVQKDYDD
                     IIEVVPEAATFKHEEEIHEEKLVAMLDEERLRYAGSIVLGLNDALVELTGALAGLTLA
                     LQNVKLIALSGLITGIAASMSMAASEYLSTRSEETNKNPVRAAIYTGIAYIGTVTLLV
                     LPYLLFENYYLDLAIALATAVIIIAVFNYYISVAKDVSFRERFLEMAGLSLGVAAISF
                     IIGYLIRIWLGIEV"
     gene            4982..6139
                     /locus_tag="VFQ23_18070"
     CDS             4982..6139
                     /locus_tag="VFQ23_18070"
                     /inference="COORDINATES: protein motif:HMM:NF020318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HET9908562.1"
                     /translation="MKILTVDIGTGTQDIYLYDSNLDIENGFKLVLPSPTMMVHRHLK
                     QMIASRTPLLLTGHQMGGGPSAWAIEEYARAGIPIYMTPSAATTINDELEKVQGLGIK
                     IVSEDDARGLKSKVQGFELKDFDFELISKTFMDYGVSLNDVQAIAVAVFDHGNAPAGV
                     SDRQFRFDYLDERIKATNSLSAFAYLSTDIPEIMTRLQSVADSAGELPCPLVVMDTAP
                     AAVLGAGFDTVVANRRQKIICNVGNFHTLAFRLGEKGIEGVFEHHTGEIDLAKLEALL
                     RALADSSLKHEDVFNDMGHGALMYSQERFEFGKDEFDVVVTGPRRSMFQPINHRNLRP
                     YFAVPFGDMMIAGCFGLLAAIADVMPALAEAIHGSLRGGRGRGVAPWDHPA"
     gene            6152..6577
                     /locus_tag="VFQ23_18075"
     CDS             6152..6577
                     /locus_tag="VFQ23_18075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9908563.1"
                     /translation="MNQSLHLITLGVRDFETSYRFYADVLGWKPSGASQEDIAFFQTS
                     GVVFAIYPREKLAEDALVPPEGSGFAGVTLAYNTRGESEVDEIIRDLIAKGVKIIKEP
                     QKVFWGGYSSYFADPDDYRWEVAYNPSFPFDEKGNLKLD"
     gene            6645..6887
                     /locus_tag="VFQ23_18080"
     CDS             6645..6887
                     /locus_tag="VFQ23_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908564.1"
                     /translation="MNKNPWIAAVLNFFFMGLGTLYIGRRKLTGLGLTLGAIALTYVE
                     LQLRAAAPALYPIMFAAVFVANTVLAIDGYNEAQSK"
     gene            complement(7001..>7510)
                     /locus_tag="VFQ23_18085"
     CDS             complement(7001..>7510)
                     /locus_tag="VFQ23_18085"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9908565.1"
                     /translation="VVLILREVFNWSAEEVAGALDLTVDSVNNLLYRARKNLEPSQME
                     ENPAPKHYLDQFVKAWEAGDVHSLIQLLHQKATFAMPPMRVWYAGRESIQRALQNWVF
                     APGVKWKLIPTAANARPAFGIYREAEGSYIAFGLLLPIFSKEEIVEVTAFLSPQLVTR
                     FGLPENLSF"
BASE COUNT         1960 a         1725 c         1932 g         1893 t
ORIGIN      
        1 caattgatcg aatattgcaa acagaacaat caatccttgc tcgtcacctc ggcgaccgct
       61 gagaaaaccg tggacgaaat cgcgaacatc cccaaacgac ttctaattgt attcggcagt
      121 gaaaagacgg gcagttcgcc cgagattgag caggcggcaa ccctgcgcgt tcggattccc
      181 atcaatgaaa aggttgaatc gttgaacgtc tcagcggcgg cagggattac attattcaat
      241 cgaatccaat ttaatcgatc aaagagccaa taaaaaaagg atcagctttg cttgtccttt
      301 ttactaactg cttgcgccac tgtagcgttt taaccctgct ttccaaacct gtgaagcgat
      361 tagaaaactt gcaataccaa tcaacaagta catcaatacg cgacctgcat tcaaatctcc
      421 gcgcaaggcc tggagtggaa cggtcgtcgc aactgccacc gggatcacaa aggtgacaat
      481 gatccgtaac catacggggt aaactgtcac agggtaacga cctgcatcta gcagggcttg
      541 caggatagtc acgttgttgt cgaatttagt gaaccagaag gttagcgcga tcagaacgat
      601 ccataggctg taaatgacga ttgaacctga aacggtcaac aggataaagg tcaggatgtt
      661 gagcgcggta attgtgtcgc cagaaatgtt gataccgatg ctgatcaaca cgatggcgag
      721 aattaaatcc caggctctcc acaccgtaat gcgcgagaat gtaaccagga acatgctgtt
      781 gatgggctgc aggagcgtgt agtccatgga gccatcgcgg atgctctggt tgaatttttc
      841 tgtattgggc cagatgaggg cgatcatcaa ggtatgaaca agtcggaaca cgcccagaag
      901 ggcgaccaat tcgccaaaac cccagccacc aatggtttcc gtgttgctga agataatatt
      961 cagactcaac aattcccacc caagccacat gaggttgaga aggatattga ctactgtatc
     1021 cgcgcgataa gccagcgaca tctgaacatt gacttttaaa aaagccgaga gcaatttcaa
     1081 cgtttgcatg gctaggctcc gaccgccgaa aagcgtttca cgccgttccg ccatacccag
     1141 ttaaagagtg tgatagacag tatcagccat ataaaaccga cgatataacc ttgaatgatt
     1201 tgcgcgcttg agagattccc aagaatcaat tgaactggaa aataaatcag catttggaat
     1261 ggaagatact gtgcgatttc ctgaatgact tttggcatca gggtgagcgg tacaaactga
     1321 cctgagaaca acaggatcaa cgcataataa aactcgtgga tggaatagac tcgtgtcgtc
     1381 cagaaggcga gcgccgtgat cacggccgac aacaaatagc ctataaagaa tcccatgatc
     1441 atggcaggga tggcaagcaa aataccggtc agggtgacat ggctgaagtc tggtctgaac
     1501 aggaaaaaca gaatgatcca gatcggggct agtcccatga tggtcagccc tttgaaggaa
     1561 atgttgttga ccaaagcatt ggtcagcatg ggatggatcg gtttaatcag ctcgccggac
     1621 agtgtgccat cctgtatttt ataagcaaag gtatggatca cgatgttgct ggtgatctga
     1681 tctacaatca gaagggtcat gtaataagtg acgaagtcat ttgctgtaaa accgttgaca
     1741 tcccctttcg tgctggcgat gcttgtccag actgcaaggt agataatggg ggagaccagc
     1801 cagtaaagca ggtacatgat gaggttggca cgatattgcc attgttccgc ccagttcacc
     1861 tgccagagtc gttgaaagat tgtcaacata atgctttcct cacacttaac tacagttatt
     1921 gattaaacgc ctgctcgatg acatcttcga tcggcgggtc ggtgatggta atgtcatgga
     1981 tgggcaggtc agacaaaagt ccggaggtga cacgagtcac atcctcggct tttacctgga
     2041 tatattgttt ctcccagtcc tcctcgtttt ggacaggggt gccgtatttg gataaatcgt
     2101 gtgaatctac ctctgctaac ctcacgccga tcaatttgaa cggcgcgatc ttgcgtgaaa
     2161 ggtcaccgat accaccatca tatttaagtt tcccatgatg aataataatg atgcgctcgc
     2221 ataaggcagt aacatccgcc atatagtgac tggttagcag aatagtcgca ccggtacgtt
     2281 tgttgtattc ccgcaaaaac tcccgaatgc gagattgtcc cgtgatatcc agcccgatcg
     2341 ttggctcatc caggaacaag actttgggtc ggtgaagcag acccgcggcg atctcgcact
     2401 tcatccgttc gccaagcgag agattccgca ccggcttttt catcagcggt tcgagttcaa
     2461 gtaattcgtc taattcctta taagtggctt ggtactgttc gtcaggaatc cgataaatcg
     2521 cctgattgag caggaacgag tcagccgcag ggatgtccca cgacaagcga ttacgttgac
     2581 ccattacgag cgtcatcgaa cgcaaatatt ctggcttgag ttcccagggg gtaaagccca
     2641 gtacattggc gttgccatcg gtggggtgca gcaatccaga gagcatcttg agtgtggtcg
     2701 tcttgcccgc gccgtttggt ccgaggaacc caacgacttc accctgggcg atttcaaaat
     2761 taacctgttg gactgccttt acatccttat atttacgctt aaagaaactg cgcaccgcgg
     2821 cgccgaatcc accctctcgt tcggggactt gataagtctt ggttaggttt tctgcattga
     2881 tggtgattgg ggacatagat gaattgctcc ttcggggaga agcaattcta tcattattag
     2941 aacaagagtg ctagtacgtt cggcaaatat gatgaattta cgttgtcttt cacgaactga
     3001 gttattaatg actccaggct ttcgcatgat tgcgaaagcc tggagctcgc tttgtaaatc
     3061 ttaattactt tactgggaaa cccatgtgtg catatttgtt acttcacagc gtgtgccgaa
     3121 atcccgattg gtacctgaaa gaagagccag cccgataaaa ccgtccagag cacggctctg
     3181 tgacaatgta tattcgccaa caacctcgtc atcgatcagc acacgcgcat aagcatcctc
     3241 gacgataagg gtgaagtctg cctcgacggg ttcatcaaac gggttaccga acttgacaac
     3301 gcccgttccc cgtgttaatc caaccgagcg tgagtaactg gatgagctta cgttatccag
     3361 gaaaatgatt ttagcccggt caaggaatac agcataatga tcaccatttt cgttggcggc
     3421 aaacacgaaa ccacagccag aatcacctgc attgcggtat gcgctggtcc atttgaagtg
     3481 agcgctgaca taaaagtccc tggctaccgt cagaattgtt gacggccgat accagtcgag
     3541 ctgtgcccat tcttcggaag agtcctcgtg gtgagtcact tcgccatcgg tggtggataa
     3601 gtaacccagg tcaaagtatc cctgagtctg accgttaact tcctccattt gctgcgtggc
     3661 tgccagattc ggcgtccgcg tgggtctggg agtaacagtg gggcggggcg tattcgtggg
     3721 tgtaagcgta atggtagctg tcgccgtggg gtctggtgtg ctggttgaag ttggcggagc
     3781 agccgacgtg ccgcacgccg aagaaacaag cattaatagg gatgccaacc ataatacaga
     3841 acgtagtgat ttgggtttca taaaccctcc tttgaaaaga ttaacctcat aatacaagta
     3901 acatctttaa agtacaatct ttttaggaga aaataaatta cagaagactt gcaactctct
     3961 taatgctccg aaaggtgggc gcaataactt ttttgaaaaa gcccccagac ggctattcgc
     4021 aaccaggggt agaatcagat tatcaattga ggagaaaatt atgcaaattg cgaaagaaat
     4081 tcgagaaaaa atactggtgt ttcagcggct cgaaattacg gagcaccaca tttataaaaa
     4141 gcttgcaggc aagattcaat cacctgaaaa cgcaaaaatc gtcgctcaga tcgcggacga
     4201 tgaattaaga cactacgaag gattgaaaaa atattcagga caggatgttt cacctgaccg
     4261 gctgaccctg tggacttact acatcatcag ccgcatcttc ggttttacgt ttggcatcaa
     4321 actcctggaa cgcagtgaaa aaaaggtcca gaaagattac gacgatatta tcgaggtcgt
     4381 tccggaagcc gccaccttta aacacgagga ggaaattcac gaggaaaagc tggttgccat
     4441 gttggatgaa gaacgcttgc gctatgcggg gtctatcgtc ctgggcttga acgatgcgct
     4501 ggtggaactc accggcgccc tcgccgggtt gacactggcg ctgcaaaacg tcaagctgat
     4561 cgccctttca ggcttgatta ccgggattgc tgcctccatg tctatggcgg cgagtgagta
     4621 tctttccact cgttcggagg aaaccaataa aaatccggtg cgagccgcga tttataccgg
     4681 aattgcttat attggcactg tcacgcttct tgtgctccct tatctgttgt ttgagaatta
     4741 ctatctcgat ttagcgattg cgcttgccac agctgtgatt attattgcag tcttcaacta
     4801 ctatatttct gtagcgaagg atgtatcgtt ccgcgaacgt tttttggaaa tggccgggtt
     4861 gagtctgggc gtcgctgcca tctcgttcat tatcggctac ctaattcgaa tctggcttgg
     4921 gattgaagtg taataactct ccttcatgcg cgtttatact accgcatgca cacggcggcc
     4981 tatgaaaatc ttaacggttg atattggcac aggcacacag gatatttacc tttacgactc
     5041 gaatttggat atcgagaacg gattcaagct cgtcctgcct tcgccgacga tgatggtcca
     5101 tcggcatctc aagcagatga ttgcatcccg gaccccgctc ctgttgaccg gtcaccaaat
     5161 gggaggcggc ccttctgcct gggcgatcga agaatacgcg cgtgctggca ttcccatcta
     5221 tatgacacct tccgccgcca ccacaatcaa tgatgaatta gaaaaagttc aaggcctcgg
     5281 aataaagatt gtttcggaag atgatgccag gggtctaaag tcgaaggttc aaggttttga
     5341 attgaaggat ttcgactttg aattaatttc caaaaccttt atggattatg gcgtttcact
     5401 gaacgatgta caagcgattg cggtcgcggt ctttgatcat ggcaacgcac ctgccggagt
     5461 ttcagatcgt cagtttcggt ttgattattt ggatgaacga atcaaggcta cgaattctct
     5521 ttctgccttt gcttatcttt caactgacat tccagaaatc atgacgcgtt tgcagtcggt
     5581 cgctgattcc gctggtgagc tgccgtgtcc gttggtggtg atggatacag cgcctgccgc
     5641 ggtgctgggc gcaggttttg acacggtggt tgccaaccgc aggcagaaaa tcatctgtaa
     5701 tgtagggaat tttcacacgt tggcgtttcg tttgggagaa aagggaatcg agggagtctt
     5761 tgagcatcac acgggtgaga tcgatctggc aaaacttgag gctttgttgc gcgcgctggc
     5821 agatagctcg ctcaagcatg aagatgtgtt taacgacatg ggacacggcg cgttgatgta
     5881 ttcgcaggaa aggtttgagt ttgggaagga tgaatttgat gtggtcgtaa ccgggccccg
     5941 gagatctatg ttccagccca taaaccatag aaatttgaga ccgtatttcg cggttccgtt
     6001 cggggatatg atgatcgcag gttgttttgg tctgctggcc gcaatagcag atgtgatgcc
     6061 tgcgttagcg gaagccattc acgggtcgtt gcgaggtggg cgtgggcgtg gtgtcgcgcc
     6121 ctgggatcat ccagcttaag aggagacaaa tatgaatcaa agcctccatt tgattacttt
     6181 aggtgtacgt gattttgaaa catcctacag attttatgca gatgtacttg ggtggaagcc
     6241 gtccggagca agtcaggagg acatcgcatt cttccaaacc agtggagtcg tgttcgcaat
     6301 ctatccgcgt gaaaagctgg ccgaggatgc gctggttccc cccgaaggtt caggttttgc
     6361 tggtgtgact ttggcatata acacgcgcgg tgaatcagaa gtggacgaga tcatccgcga
     6421 cctgatagca aaaggtgtga aaataatcaa agagccccag aaggtatttt ggggtggata
     6481 cagttcctat tttgctgacc ccgatgatta tcgctgggaa gtggcataca atccatcctt
     6541 cccttttgat gaaaaaggaa atttgaaact cgactgaaaa agtgagaagt tcgagcgggc
     6601 tttttcgtct taacgttaaa tccaattttc aaaggagtgt tgagatgaat aagaacccgt
     6661 ggattgctgc tgtgctgaac tttttcttta tggggctggg gacgttatat attggccgtc
     6721 gcaagttgac agggctgggg ctcaccctgg gtgctatcgc gctgacctac gttgagttgc
     6781 aattgcgagc tgcggcgccc gcgctatacc cgatcatgtt cgcggcagtg tttgtggcga
     6841 atactgtgct ggcgattgat ggttataacg aagcgcagag taaataatca ttgcaggtca
     6901 ctctcccgaa gggacgccgc ttcgggacgg tgtgagcgaa gcgacacccg ggcttgacgt
     6961 gaaaatctcc agggctatcc tggagatcct tcgcttacgc tcagaatgac aaattttccg
     7021 gcaaaccaaa ccgagtgacc agttgcgggg ataagaacgc ggtaacctcg actatttcct
     7081 ctttcgaaaa gatgggtaga agcaagccga aggctatata gctaccttct gcttcgcgat
     7141 agattccaaa ggcgggacgt gcattcgcgg cggtgggaat caatttccat tttacgcctg
     7201 gggcaaaaac ccaattctgc agcgcgcgtt gaatggattc tcgtcccgca taccatactc
     7261 gcattggcgg catcgcgaag gtcgcttttt gatgcagcag ttgaatcagg gaatggacat
     7321 cccccgcttc ccacgcttta acgaattgat ccaagtagtg ttttggagca ggattttctt
     7381 ccatttgaga gggctcaagg tttttacgcg cacggtataa aagattgttg accgaatcaa
     7441 ctgttaaatc aagcgcgcca gcaacttcct ccgcgctcca attgaagact tcgcggagga
     7501 tcaagaccac
//
LOCUS       DASTJP010000615         4481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_695912, whole genome shotgun sequence.
ACCESSION   DASTJP010000615 DASTJP010000000
VERSION     DASTJP010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695912"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFQ23_18090"
     CDS             complement(<1..935)
                     /locus_tag="VFQ23_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family competence protein"
                     /protein_id="HET9908566.1"
                     /translation="MRVIMQAESLPASNRIKEQLMTHLPLMWFSLAFLAGIVLGSLVS
                     LPIWAWLVLGGIFLAIAVSLRFLPLSAGLRSASEASQGKALSLIFNPFLFLLLFALFL
                     GAARYQASVPRFDAFHISFYNDRDYDLLITGYLVEPPDYRDNYTNLRLQVTAVDTADG
                     DLSANGLLLVRAPNNQTFHYGDILRLRGRLQTPPENEEFSYRDYLATQHIHSYMSSAE
                     VTVLPGRGGNPISAVLYAIKDQSLTNIYRMFPDPEASLLAGILLGVDTGLTLELQQAF
                     KNTGTAHIIAISGFNISIIAGIFFALFSRFFGERRG"
     gene            981..2156
                     /locus_tag="VFQ23_18095"
     CDS             981..2156
                     /locus_tag="VFQ23_18095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET9908567.1"
                     /translation="MKRALLIGFVMIFAASLMLVVALYVPNSLPAHSDFSAIYNTDLA
                     LVHRIPIYDLEAVEAIAVKASGLPPDKFFLARFPYPPWYALSTFYLGLLPAKAAATLW
                     FEINLIMLFLSVWLLTDGWPGRLRLISFPLALFFLPVLGALSVGQYGFPVLLGTSLLI
                     YSLRRENVALTTLGTVLLIFKPHIGALILLSALIYLIVNKSNFRQRAIRSILIAGGFL
                     FVAGFLADSAWPVSYPQMLLNYQGEGNVSTCSECASLPVWLSRWIFDGSLGQAVLIAV
                     VLLTILIFFSYRVRSELSASPELLLNTALLITLLVSPYLYNYDFILLLVPFAFLIGQK
                     LRFIHRLIVLICYLVPTFAIAFYGRNGNISLLVVTLVITILVFLTSKPKVDVPDLAA"
     gene            2483..3394
                     /locus_tag="VFQ23_18100"
     CDS             2483..3394
                     /locus_tag="VFQ23_18100"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HET9908568.1"
                     /translation="MLRKTLLITLLIVLLSACAPQATPTAEVLTFTDGLGREISLNGP
                     AQKVISIAPSNTEILFAIGAGPQVIGRDQLSDYPAEAATVTDVGSAFEALNTELIVSL
                     QPDLVLAAEINTPEQVKQLEDLGLTVYYLKNPLTLEEMYSNLELVAQLTGHEAETTTL
                     IESLKARVAAVDEKIAPLSSRPSVFYELDATDPAKPYTAGNGTFINLLIDRAGAFNIA
                     SDIDGYPQLSLEQVVAADPAFIILGDSAYGVTPETIASRPGWENLSAVTNNQVFPFDD
                     NLVSRPGPRLVDALEQLAKLIRPELFQ"
     gene            3459..4454
                     /locus_tag="VFQ23_18105"
     CDS             3459..4454
                     /locus_tag="VFQ23_18105"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET9908569.1"
                     /translation="MRNPFLTTLLTFIAMLILSMAIGSVFIPPAELWRVITGATSDAT
                     ARTILLDIRLPRTMLIAMVGAALGGSGAAYQGLFRNPLADPYLIGVASGAGLGAVLAM
                     SFNWPYTGFGLLAVPLAAFIASLLTVYIVYLLSNIGGTVPTTSLILAGVAVSSFAVSL
                     TSFLMLRSTGEVRRALGWLLGGVSLVGWDVTLSLIPYLVIGLTALLLNGYALNVMQFG
                     DDQAAQLGLNVRRAKLFIIVAASLVTAAAVSFAGIIGFVGLVVPHIVRIWWGVDYRRL
                     IPLSIIGGASVLLFADVLARVVMSPQELPVGIVTALAGAPFFLWVLRHSKNQGLW"
BASE COUNT         1000 a         1088 c         1152 g         1241 t
ORIGIN      
        1 ccgcggcgct cgccgaagaa acggctgaag agcgcgaaaa atattccggc gatgatgctg
       61 atgttaaagc ctgagattgc aatgatgtgc gccgtgcctg tgttcttgaa ggcttgctgt
      121 agttcaaggg tcaagcctgt atccaccccc agaagaatgc ccgccagaag cgatgcttca
      181 gggtccggga acatgcgata aatattggtg agtgactggt ctttgattgc gtataaaaca
      241 gcggagatcg ggttgccacc cctgcctggc aataccgtta cctcagcact ggacatatac
      301 gaatgaatat gctgcgtggc gagataatcg cgataggaaa attcttcgtt ctcaggtggt
      361 gtctgcaacc ttccacgcag gcgcagaata tccccataat ggaaggtttg gttatttggc
      421 gcccgcacaa ggagcaatcc attggccgaa agatcgccat ccgctgtatc taccgccgta
      481 acctgaaggc ggagattggt atagttatcg cgatagtcag gcggctcaac aagataaccg
      541 gtgattagga gatcgtaatc ccggtcgttg tagaacgaga tgtggaacgc gtcaaacctg
      601 ggaaccgaag cctgatagcg tgccgcgcca agaaacagag cgaaaaggag aagaaaaaga
      661 aagggattaa aaattaagga aagagccttg ccttgcgatg cttcagaagc actacgaagg
      721 cccgctgaaa gaggcaggaa gcgtagggat acggctatag caagaaatat tccacctaaa
      781 accagccatg cccaaattgg tagtgaaacc aggctcccaa ggacgatccc cgccaggaac
      841 gcaagcgaaa accacatcaa tgggagatgg gtcatcaatt gctctttgat tcgattcgat
      901 gcgggaagac tttctgcctg cataatgact cgcatgatag catagatttg taataggagg
      961 ataaaatcca ggcgtagcca atgaagcgcg cacttctgat cggtttcgtc atgattttcg
     1021 ctgccagctt gatgcttgta gtagctcttt atgttcctaa ttcactcccc gcgcattccg
     1081 attttagtgc catttacaac acagaccttg ctctggtaca tcgcatcccc atttatgacc
     1141 ttgaagccgt ggaagcgatt gctgtaaaag cctcaggcct gccgcctgat aaattctttt
     1201 tggcgcgctt tccgtacccg ccctggtatg cgctcagcac attttatttg ggactgttgc
     1261 ctgccaaagc tgcagctacg ctttggtttg agattaatct aatcatgttg tttttatcgg
     1321 tctggttgct gaccgacgga tggcccggtc gtttgagatt gatctctttc cctttggcgc
     1381 tgttttttct acctgttttg ggggcgttat ctgtcggaca atatggcttc cctgtgttgc
     1441 ttggtacgtc tttgctcatt tattcattgc gaagagaaaa tgtcgcgttg accaccctgg
     1501 gcacggtatt gcttattttc aagccgcata tcggcgcttt gattttgcta tcagcattga
     1561 tctatttgat cgtaaataag agtaatttca gacagcgagc aatacgctca attttgattg
     1621 ccggaggctt tctcttcgtg gctggcttcc tggctgactc tgcttggccg gtcagttatc
     1681 cacaaatgct cttgaattat caaggtgagg gaaatgtttc gacctgctca gaatgtgcca
     1741 gcctgcctgt gtggttatca cgatggattt tcgatgggtc gcttgggcag gccgttctca
     1801 ttgctgttgt cttactgaca attctcatct ttttctctta tcgagttcgt tctgaattat
     1861 ctgcatctcc tgaattgctt ttgaacacag ctttgctcat cactttgctc gtcagcccat
     1921 atctttataa ctatgatttt attcttttgc ttgtgccgtt tgcctttctt atcggtcaga
     1981 agcttcgatt catacaccgg ctcattgtcc tgatttgcta tctggttccc acattcgcca
     2041 tcgcatttta tggtcgtaac ggaaatatct cgttactggt tgtaactctc gtgataacta
     2101 ttcttgtttt tctaacgtcg aaaccaaagg ttgacgttcc tgacctcgcg gcataaaata
     2161 gattcaatct tatacgatca gcaagcggcg aagctggtga gattccagca ctgtcgcgca
     2221 actgtgaatt tagttagata gttacagagt tggatagttg atggttgaac tacaagacta
     2281 ttgaactaac aaactatcaa actaaggaag tcaggtcgcc cgctctggtc ggttcaccac
     2341 actctcgcgg aaaggaggtg gaggacgacg ctgttcggcg caaagcgagt ctttccaacc
     2401 tccccggctt cggctgggga ttttgattct tatttcaact gtagtgacga cttaagtcgt
     2461 ccctacctaa tcaggagaag agatgttacg caaaacctta cttattactt tactcattgt
     2521 gctactgagt gcttgtgcac ctcaggccac accaacggcg gaagtgttga cgttcaccga
     2581 tggacttgga cgcgaaataa gcctgaacgg tcctgcccaa aaagtgatct caattgcacc
     2641 gtcgaatacg gaaattctct ttgcaatcgg cgcgggccca caggtaatag gacgcgatca
     2701 gctttcggat tatcccgccg aagctgcaac ggtcactgat gttggctctg catttgaagc
     2761 gttgaatacc gagttaatcg tttcgcttca acctgatctg gtgcttgctg ccgagatcaa
     2821 tacgccagaa caggtcaaac aactcgaaga cctgggtctg accgtttatt acctgaaaaa
     2881 cccgcttacg ctggaagaaa tgtatagcaa ccttgaactt gttgctcaat taaccgggca
     2941 tgaggcagaa actaccacgc tgatcgagtc actgaaagca cgtgtcgctg cggtggatga
     3001 gaagatcgct ccgctcagtt cgcgtcccag cgtcttttat gaattggatg ccacggaccc
     3061 ggcgaagcca tatacggcgg gtaacggtac cttcattaac ttattaatcg accgcgcggg
     3121 ggctttcaat attgcttctg atattgatgg gtatccacaa ctaagcttgg agcaggtggt
     3181 cgccgctgac cccgccttca ttattctcgg tgactctgcg tatggagtca ctcccgaaac
     3241 cattgcatct cgcccaggct gggaaaattt gagcgccgta acgaacaacc aggtctttcc
     3301 tttcgacgat aatctggtca gccgaccggg gccacgtctg gtggatgctc tcgagcaact
     3361 ggcgaagctg ataagaccgg agctctttca atagtcaaac agacttcaga agcactgaag
     3421 aacaataagg cttctgaagt ctgttatatt tttccctgat gcgcaatccg tttcttacca
     3481 cgctgctgac attcatcgcg atgctcatac ttagtatggc gattggttcc gtattcattc
     3541 caccggcaga attatggcgt gtgatcactg gtgcaacttc agatgcaacg gctcggacga
     3601 ttcttttgga tatccgtctg ccgcgcacca tgttgatcgc catggttggg gcggcattag
     3661 gtggaagcgg cgcggcctat cagggattgt tccgtaatcc actggcggac ccgtatttga
     3721 tcggcgtggc ttcgggggcc gggttgggcg ccgtgctggc gatgtccttc aattggccct
     3781 ataccggatt tggcctcctg gctgttccat tagctgcttt catcgcaagt ttgcttaccg
     3841 tctacattgt ttatttgctc tcgaatattg gcgggaccgt gccaaccacg agtctgattc
     3901 ttgcgggggt agcagtctcc tctttcgcgg tttcgttaac ttcgtttctg atgctgcgct
     3961 ccacggggga ggtgcgtcgt gcgttgggtt ggctgctggg cggagtcagc ctggtcggct
     4021 gggacgtgac cctctcactt attccctacc tggtgatcgg gttgacggcc ttgctcctga
     4081 acggatatgc attgaacgtc atgcaatttg gtgacgacca ggccgctcaa ctcggattga
     4141 acgttcgccg cgcaaaactc ttcatcatag tcgctgcatc gcttgtgacg gcggccgcgg
     4201 tttccttcgc ggggattatt ggctttgtgg ggcttgtggt accgcatatt gtacgcatct
     4261 ggtggggtgt ggactaccga cggctcatcc cgctgagtat cattggaggc gcttctgttt
     4321 tgttgtttgc ggatgtactg gcacgcgtgg taatgtctcc gcaggagttg cccgttggaa
     4381 tcgtcactgc cctggcaggc gcgccgttct tcctttgggt gttgaggcat tcaaagaatc
     4441 aagggttatg gtaatgctaa atcctaaagg tttatcgatg t
//
LOCUS       DASTJP010000616         6251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_577993, whole genome shotgun sequence.
ACCESSION   DASTJP010000616 DASTJP010000000
VERSION     DASTJP010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577993"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..647)
                     /locus_tag="VFQ23_18110"
     CDS             complement(21..647)
                     /locus_tag="VFQ23_18110"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET9908570.1"
                     /translation="MNSTEWVKVAARAGMVGPFLFGIILSTLTILKYDFLLSIGWHPF
                     RAPTLDWPSGLALGDYGWIMTATFITSGFMMILFASGLRLALPPVRTALIGTILLSLA
                     GLGLIGLASTTDPTLTTVTRTWHGLLHDASFVILGLTLMPAMIALGFAFRDNQYWSHL
                     SLYTWITVALAFPAFWLKGAAFYVFLLAVLIWCEVIAFQLGKWTQIHI"
     gene            complement(644..997)
                     /locus_tag="VFQ23_18115"
     CDS             complement(644..997)
                     /locus_tag="VFQ23_18115"
                     /inference="COORDINATES: protein motif:HMM:NF018672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET9908571.1"
                     /translation="MTTLANITIAIVAVLHLGFLALEMFFWDHPFGRKRFKMTPEYSR
                     ASASLAANQGLYNGFLAAGLFWGLWTGQFAVKMFFLICIVIAGIYGGLTTRRTILYIQ
                     ALPALLALLLLYLSR"
     gene            1141..1914
                     /locus_tag="VFQ23_18120"
     CDS             1141..1914
                     /locus_tag="VFQ23_18120"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9908572.1"
                     /translation="MPYEMILTETHGRVGLITFNRPQAMNALNNQLMRELMDALEAFD
                     KNDDIGAMVISGNEKAFAAGADIKEMADKSIHQMMDTDHVAVFGRIRTIKKPVIAAVS
                     GFALGGGCEIALSCDMIIASETAKFGQPEITIGVIPGAGGTQRLTHAVGKAIAMEMVL
                     NSRTLSAQEALQYGLVNRVVPLSDYLNEALKLAEDIAGRAPLAVAAGKRMINQSYERF
                     LSDGLAQEKQVFYNLFASEDQKEGMRAFVEKRKPEWKGK"
     gene            1916..2698
                     /locus_tag="VFQ23_18125"
     CDS             1916..2698
                     /locus_tag="VFQ23_18125"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HET9908573.1"
                     /translation="MLTYAELKSAFEDLKIGDRPVIAHASFRRFGEVEGGAATVLHAL
                     IDSSSGLIMPTFTYNTMVTPEVGPPNNGIRYGSDRDNNRRAEPFHIMMPSDKMMGILP
                     WVLLQQDGAVRTSHPILSFGGVRADGILATQTLYEPLAPIRILEEQDGWVVLINVDHT
                     VNTSIHFAEKLAGRRQFIRWALVENRVVECPGYPGDSSGFQAIEEYIGPDIRRVDVGQ
                     GFLQAIPLQILFSAVKEVIKKDPLALLCEREDCERCNAVRGH"
     gene            complement(2692..>6251)
                     /gene="dnaE"
                     /locus_tag="VFQ23_18130"
     CDS             complement(2692..>6251)
                     /gene="dnaE"
                     /locus_tag="VFQ23_18130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET9908574.1"
                     /translation="EIPRSLMDDKPEEAVRRMNWYYDVFGPDRFFIELQQHNIKEITD
                     LNRKLVEMGARYSAKYVATNDVHYINQDDSRLQDILLAIQTQTLLSDPERMRMTDDSY
                     YLRTPAEMSRLFAEVPEALSNTLLIAERCNVDLSFKGYHLPDFPVPDGFTAETYLRYL
                     CEAGAQKRYGDRAQSQQVRERIDYELGIVHKMGFDAYFLIVWDLCRHARDHEIWYNAR
                     GSAAGSIVAYVLEITIVDPIQHDLLFERFLNPGRISMPDIDLDFRDDRRAEMLEYCTR
                     KYGSDKVAQIITFGTMGTKAALRDVGRVMDIPLPEVDRVAKLVPFVSGRTTTMQDALE
                     VPEFKKIYNEGSHLRELIDIATKMEGTVRNAGTHAAGVVISDKPLLEYLPLHRPTSNS
                     EETPIKTVTQFEMGILDSLGMLKVDFLGLITLSVMAKACELIEQRHGIKFDLSNIPLD
                     DPKSFELMGNGQTAGVFQVEGGGMTRWLVQMKPKTLDNIIAMVALYRPGPMAFIPDYI
                     ARMHGKAEVEYRHPALAPIFQDTFGIPVYQEQLMRAAVELAGYTPSESDELRKAISKK
                     NKKDIDKHREKFVKGAVNQGMEKSIAESIYSDWEEFARYGFNKSHAADYGVIAVQTAY
                     LKSRYPAEYMTALLSASAGITEKVAFYISDARSMGVNVLPIELNTSRWDFAIEEVAEK
                     PNIRFGFGAVKNVGQGAVDLILIERDQNGPFKDLNDFARRVDLRAVGKRALECLIKVG
                     AMDSFGNRAALLASLDRIISISSNHFRAAEAGQMSLFGAATGVVDEITLPDVNNVDKR
                     EMLNWERELIGLYISDHPLTPYQKTFTQIVSYFSGQLGEAQHEEKTRVAGLITAIRPY
                     MTKTNKPMGFVTLEDIQGNIELVLFPKTWGTYREKLSVGQIVIVEGKVDMNSAPPKVL
                     VDTIRTEIKILESVDDPSPIQQSLYSQDYGLLGSPLAKPAPAQVQKATPQPKPAPVAT
                     KPATKAVSEPASAYPVEDSFDDIPPPPDNFPDDWDTQWQPSFENAELAASPKALNQEN
                     IPITKPPEQVTQPIDEPKEEAASPLLKTEQATTVYEKLIVQPIEDLPAETLSMPPSLY
                     VPLAKEEKDKEHRPQQITLTLRSTGDKDWDKRKIKTLYGTLISFHGRDRFSFQIFENG
                     TGHLIDFPNDTTRVCSEMLERLKKLLGQENWRVEEITFQ"
BASE COUNT         1577 a         1543 c         1610 g         1521 t
ORIGIN      
        1 tctatcattt ttagagtttc tcatatatgt atttgcgtcc atttgcccaa ctgaaacgca
       61 atgacctcac accagatcaa tacggcaagc agaaaaacat agaaagcggc gcctttgagc
      121 caaaaagcgg gaaatgccag cgcaaccgtg atccatgtat aaagagataa atgactccag
      181 tactggttat cacgaaaggc gaacccgagg gcgatcatgg cgggcataag ggtgagacca
      241 agtattacaa aagaagcgtc atgtaagagt ccatgccagg tgcgagtgac tgtagtgagt
      301 gttgggtcag tggtagaagc taaaccgatg agacctaacc cagctagaga gagaagtatc
      361 gtgccgatca aagctgtacg aaccggaggc aaggcaaggc gtagcccgga ggcaaacagg
      421 atcatcatga atccgctggt gatgaatgtg gcagtcatga tccatccgta gtccccaagt
      481 gcaagtccac tgggccaatc gagtgttggt gcacgaaagg gatgccaccc aatcgaaagc
      541 agaaaatcat atttaagaat tgtgagtgtc gaaaggataa ttccgaatag gaagggacca
      601 accatccctg cgcgtgccgc gactttgacc cactccgttg agttcatctt gataaatata
      661 acaggagtaa tgcaagcaac gcaggcagcg cctggatata caaaattgtg cgtctggtgg
      721 tcagtccgcc atatatccca gcaatgacaa tacagatcag aaagaacatt ttcactgcga
      781 actgtccggt ccaaagtccc cagaagagac ctgcagcgag aaagccgtta taaagcccct
      841 gattcgcggc cagagaggcg gatgccctcg aatactctgg cgtcatcttg aagcgctttc
      901 tgccaaatgg atgatcccag aagaacattt caagcgccag aaagccaaga tgcaggactg
      961 cgacaattgc aatcgtgatg ttcgccagag tggtcatgtt gtctccttta ttttgatcaa
     1021 ttcaatagta gcataaattt gcttctcgtg tagaatcctt gtttgtcact gcgagtggcg
     1081 aagcagcatg aagtaagagg aaattacttg ccggaggctc gcaatgactg aggagtcttt
     1141 atgccatacg aaatgatcct gactgaaaca cacggacgcg tgggtctcat tacatttaac
     1201 cgaccacaag cgatgaacgc gctcaacaac cagctcatgc gcgagctgat ggacgcgctg
     1261 gaggccttcg ataaaaatga cgatatcggt gcgatggtga tttcaggcaa cgaaaaagcc
     1321 tttgcagcgg gcgctgatat taaagaaatg gcagataaat ccattcatca gatgatggat
     1381 accgatcatg tggcggtgtt tggcaggatt cgaactatta agaagccagt gattgctgcc
     1441 gtatcaggtt ttgcattagg cggcggctgt gagatcgctc tatcctgtga catgatcatt
     1501 gcctccgaaa cggccaagtt tggtcaacca gagatcacga ttggcgttat cccaggggcc
     1561 ggcggcacac aacgtctcac gcacgcggtc ggcaaagcaa tcgcaatgga gatggtcctt
     1621 aacagccgca cgctttctgc acaggaagct ctacaatacg gacttgtcaa tcgcgtggtt
     1681 cccctcagtg attatttgaa cgaagcacta aagctggcag aagatattgc tggacgcgcg
     1741 ccgctggctg tggccgccgg gaagaggatg attaatcaat cttacgagcg ttttctcagc
     1801 gatggactcg cgcaggaaaa gcaggtcttc tataatttat ttgccagcga agatcaaaag
     1861 gaaggcatga gagcatttgt cgaaaaacgc aaaccagagt ggaagggaaa ataagatgct
     1921 cacgtacgcg gagcttaaat cggcatttga agatttaaag atcggagacc ggcctgtcat
     1981 tgctcacgca tccttcagac gattcgggga agttgaagga ggtgcagcca ccgttcttca
     2041 tgctctgatc gattcttcaa gtggcttgat catgccgact tttacctaca acactatggt
     2101 cacgccggaa gtgggacctc ccaacaacgg gattcgatat ggcagtgacc gcgataataa
     2161 tagaagagct gaaccgtttc atatcatgat gccttctgac aaaatgatgg gcatcctgcc
     2221 gtgggtactt ttgcaacagg atggcgccgt tcgtacttca cacccaatcc tttcctttgg
     2281 tggcgttcga gcggatggca tcttggcaac acagactttg tacgaacctc tggcgcccat
     2341 ccgcatactg gaagaacagg atggctgggt tgtgctgatc aatgtagatc atacggtgaa
     2401 taccagcatt cactttgccg aaaaattggc gggtcgaagg caattcatcc gctgggcgct
     2461 ggttgaaaac cgtgtggtgg agtgcccagg ctatccaggc gattcatcag gatttcaggc
     2521 aattgaagaa tacatcgggc cggatatacg ccgcgtggat gtcggccaag gctttcttca
     2581 agcgattcct cttcagatat tattttccgc cgtgaaagag gtaataaaaa aagacccgct
     2641 ggcgttgctt tgcgagcggg aggattgcga acggtgtaac gcggtaagag gtcactgaaa
     2701 cgtaatctct tcgacacgcc agttctcttg ccccaataac tttttcaatc gttcgagcat
     2761 ctcagagcag acacgcgttg tgtcgttggg gaaatcgatc aggtgccccg tcccattttc
     2821 aaaaatctgg aaactgaaac gatccctccc atggaaggag atcagtgtac cgtagagagt
     2881 tttgatcttg cgtttatccc aatccttgtc acctgtcgag cgcaaggtga gcgtgatctg
     2941 ttgcgggcgg tgttctttat ctttctcttc ctttgccagc ggaacatata acgaaggtgg
     3001 catagaaaga gtttctgccg gaagatcctc aataggctgg acgattaact tttcatacac
     3061 ggtcgttgct tgttccgttt tcaacagcgg tgaagcagct tcttctttcg gctcatcaat
     3121 cggctgagtg acttgttcag gcggcttggt gatcgggatg ttttcttgat tcaatgcttt
     3181 agggctggcg gcgagctctg cattttcaaa agaaggctgc cattgagtat cccagtcatc
     3241 ggggaagtta tctggcggag gtgggatatc atcaaaacta tcttcaactg gatatgccga
     3301 agcaggctca gatacagctt tcgtggcagg cttggtggca acgggagcag gtttgggctg
     3361 aggagtcgcc ttttgaacct gggccggcgc tggcttcgcg agcggtgaac ccagcaagcc
     3421 gtaatcctgc gaatagagtg attgttgaat tggtgaagga tcatccaccg attccagtat
     3481 ttttatctcg gtccgaatcg tatctaccag gactttcggc ggcgcgctgt tcatatccac
     3541 tttgccttcg acgatcacga tctgaccgac actcagtttc tcacgatagg ttccccaggt
     3601 ttttgggaaa agtaccaatt cgatgttgcc ctgaatgtct tcgagcgtga caaagcccat
     3661 aggcttattg gtcttggtca tatacgggcg gatggccgta atcaaaccag ccacgcgtgt
     3721 tttttcttca tgctgcgctt cgccaagctg acctgaaaaa taactgacga tctgtgtaaa
     3781 tgttttttga tacggggtca gtggatggtc ggagatataa agaccgatca gttctctctc
     3841 ccaattgagc atctcgcgtt tatccacatt attgacatcg ggaagagtga tctcatccac
     3901 cacgccggtc gccgcgccga acaggctcat ttgccctgct tccgccgcac ggaagtgatt
     3961 gctactgatg gatatgatgc ggtcaagcga ggcaagcaag gcggcgcggt taccaaacga
     4021 atccattgcc ccaactttga tcaaacactc aagagctcgt ttgccgaccg cgcgcaaatc
     4081 aacgcgacgc gcaaagtcgt tcaggtcttt gaacgggcca ttttgatcac gctcgataag
     4141 gatcaagtca actgcgccct gtcccacgtt ctttaccgcg ccaaacccga agcgaatgtt
     4201 gggcttttca gctacctctt cgattgcaaa atcccagcgg gatgtattga gttcaattgg
     4261 caaaacgtta acgcccatac tgcgcgcgtc agagatataa aacgcaacct tctctgtgat
     4321 acccgcagat gcagagagta acgctgtcat atattctgca ggataacgcg atttgagata
     4381 ggccgtttgg accgcgatga caccataatc cgccgcatgg gatttgttga agccataacg
     4441 cgcaaactct tcccaatcgc tgtaaatcga ttcggcaatg gatttctcca tgccctgatt
     4501 gacagcaccc tttacgaact tctcgcggtg tttatcaata tctttcttat tcttcttgga
     4561 aatcgctttg cgtaactcgt ctgattcaga gggggtgtat cccgccagtt ccacagccgc
     4621 gcgcatcagt tgttcctgat aaacggggat accaaacgta tcctgaaaaa tcggtgccaa
     4681 agcagggtga cgatattcaa cttctgcctt gccatgcata cgagcaatgt aatcaggaat
     4741 gaatgccatc ggtcccggac gatagagtgc caccatggcg ataatattat caagtgtctt
     4801 gggcttcatc tgcacgagcc agcgcgtcat gccgccgcct tccacctgga agacgcctgc
     4861 ggtctgcccg ttgcccatca gttcaaatga tttggggtcg tcaagcggaa tattactcaa
     4921 atcaaactta atgccgtggc gttgctcgat cagttcacag gctttcgcca tgacacttaa
     4981 ggtgatcagc ccgaggaaat ccactttcaa catgccgagc gaatcgagaa tacccatttc
     5041 gaactgtgtc accgttttta tgggcgtttc ttccgaattc gatgttgggc ggtgcagcgg
     5101 caaatattcc agcaatggct tgtctgagat caccacaccg gcagcgtgcg tacctgcatt
     5161 gcgcacggtg ccttccattt tggtagcgat atcgatcaac tcacgtaaat gtgagccttc
     5221 gttatatatc ttcttgaact ccggcacttc gagggcgtcc tgcatggttg tcgtacgacc
     5281 cgaaacaaac ggaaccagct tggcaacgcg atcaacctcg ggtaatggga tatccattac
     5341 acgacccaca tcacgcaaag cggctttcgt ccccatggtg ccgaaggtga tgatctgagc
     5401 gaccttatcg ctgccatatt ttcgcgtgca gtattcaagc atctcggcgc ggcgatcatc
     5461 gcggaagtca aggtcgatat cgggcatcga gatgcggccc gggttcagga atcgctcaaa
     5521 gagcagatcg tgctggatcg ggtcgacaat cgtgatctcc agcacataag caacaatcga
     5581 acctgcggct gaaccgcgcg cgttgtacca gatttcatga tcgcgggcat gacgacacaa
     5641 atcccatacg atcaggaagt aggcatcaaa tcccattttg tgaacaatgc ccaactcata
     5701 atcgatcctc tcgcggacct gttgactctg cgcgcgatct ccataacgtt tctgggcccc
     5761 cgcttcacac aaataccgga gatacgtctc agcggtgaac ccgtcaggga ccgggaaatc
     5821 tggcaggtga taacccttga acgacaaatc cacgttgcag cgttcggcaa tgagcaatgt
     5881 gttacttagt gcttcgggaa cttcagcaaa aagccggctc atttctgcgg gcgtgcgcag
     5941 gtagtatgag tcgtcggtca tgcgcatgcg ttcggggtct gagaggagcg tctgcgtctg
     6001 gatcgcgagc agaatatcct gtaagcgcga gtcgtcctga ttgatgtagt gcacgtcgtt
     6061 cgtggccaca tactttgctg aataacgtgc ccccatttcc actaacttgc gattcaagtc
     6121 agtaatttct ttgatattgt gttgttggag ttcgatgaag aaccggtccg gaccgaacac
     6181 gtcgtagtac cagttcatcc gccggactgc ctcttccggc ttgtcatcca tcagtgaccg
     6241 tggaatctca g
//
LOCUS       DASTJP010000617         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1243355, whole genome shotgun sequence.
ACCESSION   DASTJP010000617 DASTJP010000000
VERSION     DASTJP010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243355"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..434
                     /locus_tag="VFQ23_18135"
     CDS             162..434
                     /locus_tag="VFQ23_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908575.1"
                     /translation="MLTFDAYPTSALGFRRTSSERSGLHRLFAAAIVSNQFRETLLHE
                     PEAALANGYLGQTFALTDMEETIIKSVRANTLADLAQKVNQALRSI"
     gene            952..2040
                     /locus_tag="VFQ23_18140"
     CDS             952..2040
                     /locus_tag="VFQ23_18140"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9908576.1"
                     /translation="MQQFSALRSIDLAIASGLDLNLLLSMLLDQVTLLLRIDAASILL
                     LNSKNNMLEFTAGKGFYSNSLQHTHLKLGEGYAGRVAVERKMLNIPDLMENGSEFSRS
                     PHFFDERFVAYYGVPLMAKGRILGVLELFHRSPLKPDADWLDFLVMIAGQAAIAIDSG
                     LMFKEVQRSNMELNLAYDATIEGLSRALDLRDKITEEHARRVTETTMGLAIRLGVGGS
                     DLVHIRRGAILHDIGKVAIPDGILFKPGPLAEDEWKIMRRHPSIAVDLLSPVTYLAPA
                     LDIPHWHHEKWDGTGYPDALRGNQIPFPARLFALVDVYDALISDRPYRLAWSKQDAVD
                     YIESQSGKHFDPKIVPEFLDLIRKNGIH"
     gene            2114..2190
                     /locus_tag="VFQ23_18145"
     tRNA            2114..2190
                     /locus_tag="VFQ23_18145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2148..2150,aa:Arg,seq:gcg)
     gene            2485..>3351
                     /locus_tag="VFQ23_18150"
     CDS             2485..>3351
                     /locus_tag="VFQ23_18150"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9908577.1"
                     /translation="MKTLATIDTGLDYSAIEHADLSPWTKYKYRRELAALKFAGINPL
                     DYQALTEYAAGLKSSRRAFLKSALRLVSMRYEQDMKAQATPANVAAVQAGIFRLESMR
                     DAVKVQTHKGTKAHTWLTRKQVTEITGLCGDDIEGKRDWIVLGLLLGAGLRREELKWL
                     TFDELKKIPGKRGKLRDVLEVKGKGAKDRVIPIKKLLADRLRAWKEIAGDGFVARSLG
                     MKKQLGNSMSSVAIFQLVNKYGSKIGLPQLAPHDLRRTYAQLGYEAGVTITQMSTLLG
                     HSSVATTQKYLDL"
BASE COUNT          864 a          800 c          842 g          845 t
ORIGIN      
        1 tgatggcgga catggcccac gcggtttttc acctacagat ggtggacacg gtccacgcgg
       61 cttttcaccc actgatggcg gacacggccc acgtggcttt tcacctacag acggtggaca
      121 cggtccacgt taatttagtt atacaggaga gttaactgac tatgcttact tttgatgctt
      181 accccacttc agcgttgggc ttccgacgga cttcatctga acgatcgggc cttcaccgac
      241 ttttcgcagc agcaatcgtc agtaaccagt tccgcgagac tctgcttcac gagcctgaag
      301 cagcactggc aaatggatat cttggtcaga cgtttgcact aactgatatg gaagaaacga
      361 tcattaagtc cgtccgtgcg aatacactgg cggaccttgc tcaaaaggtg aatcaggcat
      421 tgagaagcat ctaatccact aaaacaacac gtcaggctta cttgtgcaac caaggcactg
      481 atgtcatgtt gagcaacgcg gcgaggctgg ggcgcggtga ggataggaat catcgtgccc
      541 gcgttcattt aattgcacaa aatgaggtaa tctttggaaa atctgtctgc ctctgatcaa
      601 aaacttgaaa cttcacttca agaattttca gacctaaata ttgttctcga tactaatggg
      661 gtagttctcg attacaagtt aggtccgttt gctcaatacg gattcccaag aattactctt
      721 caccaaagaa tacaggatct tttcagtaac gacctgagag aaaattttga acgtgtttta
      781 cataccaccc agcataaagg cactgtaaat acatttcgtt attcgctgcg gatttctaat
      841 agaattcggt ggtttgaagc gcgcctggtt cccctttcaa aatcacaaat cctgtttgtt
      901 tctcgcgata tcacagaatg tcacgagttg gaaaatagat cgatacgaca aatgcagcaa
      961 ttttctgcgc ttcggtccat tgaccttgca atcgcttcag ggctggatct gaacctgctg
     1021 ctctccatgt tattggatca agtcactttg ttgttgcgaa tcgatgcagc ttccatcctg
     1081 ttgctgaatt ccaaaaataa tatgcttgag tttacagccg gcaaaggctt ttactcaaac
     1141 agcttacagc atacccattt gaaactaggg gaaggatacg ctggacgcgt agcagttgag
     1201 cgaaagatgc tcaatattcc ggaccttatg gagaatggat cggaatttag ccgctcgcca
     1261 catttcttcg atgaacgatt tgttgcatat tatggcgtac ccttgatggc aaaggggcgt
     1321 attttaggcg tacttgagtt gtttcaccgt tccccgctaa agcccgatgc tgactggctt
     1381 gattttctgg taatgattgc aggtcaggcg gcaattgcga ttgatagcgg tttgatgttc
     1441 aaggaagtgc agagatcaaa catggagcta aacctggctt atgacgccac gatcgaagga
     1501 ttatcaaggg cattggatct ccgcgataaa ataacggagg aacatgcccg ccgtgtaacg
     1561 gaaactacga tggggttggc aatccgcctt ggtgtgggcg ggtcagattt agttcatatt
     1621 cggcgaggtg ccattctgca tgacatcggc aaagttgcca tcccagatgg gattctgttc
     1681 aaacccggtc cactggctga agatgaatgg aagatcatgc gccggcatcc atcgattgct
     1741 gtggatttgc tttctccggt aacgtacctg gcgcctgcgt tggatatccc gcattggcat
     1801 catgaaaaat gggatggtac aggctaccct gatgcactgc gcgggaacca gattccattc
     1861 cctgccaggc tttttgcgct ggtggacgtc tacgacgcct tgatatcaga ccgtccctat
     1921 cgacttgcct ggtccaagca ggatgcagtc gactacattg aaagtcaatc tgggaaacac
     1981 tttgatccaa agattgttcc agaattcctg gatttgattc gaaaaaacgg tattcattga
     2041 tattttcagc cgatccatcg cttctcttga cctgactctg attatgaatt aaaatccctg
     2101 cctgccgttt tatgccctcg tagctcagtg gattagagcg cttgcttgcg gagcaatagg
     2161 tcgcgtgttc gagtcgcgcc gagggcgcca ttgtggaaaa cgtcgtcatt tgacggcgtt
     2221 ttttttgtgg ctttgaagca caaagcataa caacgtcaaa agcagtgatg gtctgtcata
     2281 tctatcagcc aacggtcgga tcataagcaa cctcgggcgc ttctggcatg catgtgaacg
     2341 attccccgat taacaacctg aaaacctgac aaagtgcctg gttgacaccc tctgcaactc
     2401 agggtaaaat acttacgtat ataataattt acgtaactgt tcggcattta ttccgtcatg
     2461 ggagaaatgt aaaggaataa atagatgaaa acactcgcaa caattgacac cgggctcgac
     2521 tattcagcca ttgaacacgc cgacctgtcc ccctggacaa aatacaaata ccggcgcgag
     2581 ctggcagcgc tgaagtttgc aggcataaac ccgttggatt atcaggctct gacagaatac
     2641 gcggcgggct taaaatcgag ccgccgcgcc tttctcaaat cggcattgcg tctcgtatcg
     2701 atgcgatatg agcaggatat gaaagcccag gcaacacctg ccaatgtcgc tgccgtgcag
     2761 gccggcattt tccgtctgga gtcaatgcgc gacgcggtca aggtccaaac acataagggc
     2821 acgaaggcgc acacctggct cacccgaaag caagtgacag agattaccgg gctgtgtggt
     2881 gacgacattg aagggaaacg ggattggatc gtccttggct tgttgctggg cgccgggctg
     2941 cgccgtgaag aactgaaatg gttaaccttt gacgaactca aaaaaatccc ggggaagcgc
     3001 ggcaaactgc gcgatgtgtt ggaagtgaaa ggcaaaggag caaaggatcg ggtcatcccg
     3061 ataaagaaac tcctggcaga tcgtttgaga gcctggaagg aaatcgcagg cgacggcttc
     3121 gtagcgcgat ctctgggtat gaaaaagcag cttggaaatt cgatgtcctc cgttgctatc
     3181 ttccagctcg tcaacaagta cgggagcaag atcggattac cccagctcgc gccgcatgat
     3241 ttgcgccgaa cgtatgcaca gctgggctat gaggccggtg taacgattac gcagatgagc
     3301 acgctgctgg gtcactcctc tgttgccacc actcaaaagt atttagattt a
//
LOCUS       DASTJP010000618        10088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_155516, whole genome shotgun sequence.
ACCESSION   DASTJP010000618 DASTJP010000000
VERSION     DASTJP010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155516"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFQ23_18155"
     CDS             <1..436
                     /locus_tag="VFQ23_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908578.1"
                     /translation="SAIICWDADFPNAVKQAGIQNVDLLFVPSNDWAAVKDIHAGMAS
                     FRAVENGLSIYRQTGSGVSIVTDALGRSIHRIDSTEETATINFAAVQMVTTPLNSVAT
                     VYPIIGDAFGNVMLVLFVGLLLGLVLSRKRFAARLKVEPLSA"
     gene            541..999
                     /locus_tag="VFQ23_18160"
     CDS             541..999
                     /locus_tag="VFQ23_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908579.1"
                     /translation="MESTFESPSESELLQSLRSRITVIEAIELAVIGFALWCLITIAS
                     PKTPDTSFVDLILFLAVTQGAILGSWLIYNLYIRIARAGGRFVDLSVWILATLLTSSY
                     TFWAWSILDLNRLVLAKLVYRGEAPVEFAWSSQSKQVRKAWERARRANNV"
     gene            complement(1098..1448)
                     /locus_tag="VFQ23_18165"
     CDS             complement(1098..1448)
                     /locus_tag="VFQ23_18165"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HET9908580.1"
                     /translation="MSKNKTVWSSEQGDLRKKKHITPDPSPGRRGESLPPQQQTIYLH
                     RESSGRGGKAVTLVKNLVLSNDEMKSLAKKLKQECGTGGTIKDDVIEMQGEQREKIAA
                     LLRTLGYKVKIAGG"
     gene            1601..1780
                     /locus_tag="VFQ23_18170"
     CDS             1601..1780
                     /locus_tag="VFQ23_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908581.1"
                     /translation="MNQIFDWSVSVLVYWATIFGISYKEINVWVFLILWPILTAILIG
                     IILMQHRRIRRLSKE"
     gene            1791..2036
                     /locus_tag="VFQ23_18175"
     CDS             1791..2036
                     /locus_tag="VFQ23_18175"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HET9908582.1"
                     /translation="MSNLVEIRSYNLKPGAGDEFHYLFLEEAFPMLQRWNMDVVSYGP
                     SLHDKNSYYLIRRFETLAHREESENAFYGSDEWRAGP"
     gene            complement(2151..2879)
                     /locus_tag="VFQ23_18180"
     CDS             complement(2151..2879)
                     /locus_tag="VFQ23_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908583.1"
                     /translation="MNYYLGGYYLVEGVSRPSYSATFLPSMIWSISNCISPIFPDSWG
                     LPWVSTRAEKLLATQRRLALSDEQFKEMQAWVDTAFNEDRFGWPNIWLNLQDAQEYYK
                     RYLQAIPQIKLLAIGIPTHYFDECLEASEPDPGRGKAGLYLRLLKPQKLDQLDKALGF
                     ELLNVEYGNVNHSFLCNHLEKEYQTMGLTFNEHGLLATYEDAKRAEEFTNLDTAGAEP
                     GPWFPWLIVEFPLTSESVKSDEND"
     gene            complement(2948..3661)
                     /locus_tag="VFQ23_18185"
     CDS             complement(2948..3661)
                     /locus_tag="VFQ23_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908584.1"
                     /translation="MPKHTYLLLALSAFLVTMCRAYDQGGPVVSANGLSCQNYSVFPG
                     GGGAECFYSCPDKTVRQPVIAENFSPDSELYSASKAELDQQLCGMASAPTATTMPVNT
                     DTPLATLDSVPSPTVTAAAEASPTLEAVVPTSSSPLLTSNITMCDQTVDLISFRITDT
                     APDLTDRDLTVQISDQETACAVNPVNTSLLTCDLPVTITFPARVLVTLDGAVVNDFMY
                     DGVGCIIVDTAIPNNTQTP"
     gene            3797..4993
                     /locus_tag="VFQ23_18190"
     CDS             3797..4993
                     /locus_tag="VFQ23_18190"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9908585.1"
                     /translation="MEQDYKKQVHDQILHQGAFVRGVFSGGQKGASLPWVKVLVRPVE
                     IKGILNLQFSYFDEKKDITKNYALEESGAKLDELLALPFRNIFIENTTGNLQINISKK
                     GKALVNQKKNPASHPAFDLSHDRQKSRLLTTENAAPFLRAVGIMTSDGKIRADMQRKF
                     KQINEFLRLLDETDSFDDFKDKPVHIVDFGCGNAYLTFAVYYYLHDVLNLDAHVAGVD
                     IKADLIERHQEKVKSLGWNQLTFHAGTISDFQPAVIPDVVLALHACDTATDDALARGI
                     QWDCRLIVCAPCCQHELQEQMSHLPMPSQLLPIFHHGILFERMGDILTDTFRATVLRI
                     MGYRTDVTQFVPIEHTAKNLMIRAVKTSPPGNTRWIEEYKNLKAFWQVTPYLERLLGE
                     AYIQFF"
     gene            5033..5902
                     /locus_tag="VFQ23_18195"
     CDS             5033..5902
                     /locus_tag="VFQ23_18195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908586.1"
                     /translation="MKLSVTNLGKQYRHDFWGLKDFSLEVEPGILGLLGPNGAGKSTF
                     MRMLATITKPTEGTIKWNEVDIVKSPDAMRAVLGYLPQDFGVYPNLNAVEFLEYMAAI
                     KGLDGASTRKRIGELLQVVNLMEAAKRPLGGYSGGMRQRVGIAQALLNDPQLLIVDEP
                     TVGLDPEERVRFRNLLSDLSGERIVILSTHIVSDVEATATHIVLLNNGKLLRQSAPED
                     LLAALDGKVWEWTVKSDDLPALKGKHIVSGTIRRSDGVQVRVVSEGKPEENAQNTSPN
                     LEDAYLYFIGGKA"
     gene            5899..7485
                     /locus_tag="VFQ23_18200"
     CDS             5899..7485
                     /locus_tag="VFQ23_18200"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9908587.1"
                     /translation="MSAARVIYHLARADFLERVRRYSFLIMLGLAAFLSYQTAVGNVT
                     MRLDQYRGEFNSAWVGSMMALISSFFLGWFGFYLVKGSVARDRETGVGQIMATTPLTR
                     PLYTFGKWVSNFAVLLAMNIVLALAGIGIQFLAGENMQLNLAALLAPFLFITLPVMAL
                     VAAVAVLFESIGFLSGGFGNVVYFFLFVFIFPLADNLSKTNPALEPLGLGLIQQSTSV
                     AAKAAFPEYDGGFVLGDPSVAEGLGFDLDPASYIFQWNGIDWTPDIVIKRFALFGLAV
                     VLALAASIFFDRFDPSRRRPRKRKGSASLSTPEPVTTSQSLSQPIHLTPLTASRNNFA
                     FIRVLISELKLLLKGQRWWWYAVAIGLFIASLVNTPENVRTFVLPFTWLWPILIWSGM
                     GNREIRHNAHQMVFSSAAPLLRQLPATWLAGFLVTVLTGSGVALKLFGAGDTVGLLAW
                     FSAAIFIPSFALALGVWSNSHKLFEVLYVTMWYLGPLNKVFPVDYLGANSDGNIGVFI
                     PCSIALIVLAFIGRARQLQN"
     gene            7668..8138
                     /locus_tag="VFQ23_18205"
     CDS             7668..8138
                     /locus_tag="VFQ23_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908588.1"
                     /translation="MKTNGLYAFAMERHGNLFQSKIFVVALILALVVAAFPGINALAA
                     GNQTTITNAGLEDEWKSKLNHLKYQGLYFDTIRLYPADFDDLSDLAQAQFYLEKYGIA
                     LRQAQTVVLNHTGFDINGRVTNQVQAAETVRNLAMYLHMMRGLRDKIEEVPSRK"
     gene            complement(8222..8434)
                     /locus_tag="VFQ23_18210"
     CDS             complement(8222..8434)
                     /locus_tag="VFQ23_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET9908589.1"
                     /translation="MSERINGTVKWFNGTKGYGFLAREGGEDVFVHFSAIQADGFKNL
                     QEGQKVEFNVEQGPKGLQASNVVVIG"
     gene            8464..8589
                     /locus_tag="VFQ23_18215"
     CDS             8464..8589
                     /locus_tag="VFQ23_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908590.1"
                     /translation="MEGTHFVLHRNRLNGEKQSGSRTIREPTWYFQSKLLVSYGA"
     gene            complement(8638..9243)
                     /locus_tag="VFQ23_18220"
     CDS             complement(8638..9243)
                     /locus_tag="VFQ23_18220"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET9908591.1"
                     /translation="MLTISIQAGGQSSRMGEDKALKLFLGRPLIQRVIDRLTPTADET
                     LVTTNRPEDYSFLNTSIRLVADLKPGRGALGGLYTAIASASHPIVAVVACDMPFASAA
                     LIEMASRLLLENEADVVIAKSEEGFEPLHAVYRRETCLPAILSAIDTDQWKVISWFPQ
                     VKVRTLTPEETLQADPLGLAFWNVNTPEEFEKAEQLALDSD"
     gene            9280..>10088
                     /locus_tag="VFQ23_18225"
     CDS             9280..>10088
                     /locus_tag="VFQ23_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9908592.1"
                     /translation="MATTIGNLLDKLTVEGEIYEKLEDNTLRCYACGHRCLIREGKRG
                     ICQVRFNQGGELRVPSGYVAALQSDPVEKKPFFHVLPSTNALTFGMLGCDFHCGYCQN
                     WLTSQAMRDPSSDAAINNIRKMTPQEMVRLAHRTNASVVVSSYNEPLITSEWAVKIFR
                     EAKAAGLLCAYVSNGNNTPEVMDYIRPYISAYKVDLKCMQDKNYRKLGGVLQNTLDGI
                     KRAHEMGLWIEIVTLTIPGFNDSNEELWDAARFIAGVSKDIPWHVTAFHKD"
BASE COUNT         2505 a         2354 c         2574 g         2655 t
ORIGIN      
        1 cagcgccatc atctgttggg atgccgattt tccaaacgcg gttaagcaag ctggaatcca
       61 gaatgtggac ttgctcttcg taccctccaa tgactgggcc gcagtgaaag acatccacgc
      121 cggcatggcg agcttccgtg ccgtcgaaaa tggcctgtcg atttatcggc agacgggatc
      181 aggcgtttcg atcgtgaccg atgccttagg gcgcagcatc catcgcattg attccacaga
      241 ggaaacagcc accataaact ttgctgctgt tcagatggtt acgacaccgc tgaattcggt
      301 cgccactgtc tatccgatca tcggcgatgc ctttgggaat gtgatgcttg tattatttgt
      361 cggattactt ctgggattag tactgtcgcg aaaacgattt gcggcacgct taaaagtcga
      421 acctctttca gcctaaatgt tatcctttct gtaaaggact ccaacaagtc tgtaagcgaa
      481 gaagatggtg atgttccata taatatttat catcatcttc aatacagaaa gagcatatcc
      541 atggaatcaa cctttgaatc tccctctgaa tctgaacttt tgcaatccct tcgaagccgg
      601 atcaccgtca ttgaagcgat tgaactcgcg gtaatcggat ttgccctgtg gtgccttatt
      661 acgattgctt cccccaaaac gcctgataca agttttgtgg atctgattct ttttctggcc
      721 gtgacccagg gagcaatcct tggctcgtgg ctgatctaca atctctatat tcgcattgcc
      781 cgcgccggag gcagatttgt ggatttgagc gtctggattc tcgcaacttt actgacgagc
      841 agttatacct tctgggcctg gagcatcctg gacttgaacc gcctggtgct ggcaaagttg
      901 gtttaccggg gcgaggcgcc ggtggaattt gcgtggagtt cacagtctaa acaggtccgt
      961 aaagcgtggg aacgagcgcg aagagcaaac aacgtttagc atacctacaa gataatactt
     1021 cataaaaaaa ctcacccgtt ggggtgagtt ttgattccgc agtcgcaatc cgtgagggga
     1081 aatggaccag aagttggtta acctccggcg atcttgacct tatatccgag tgtacgcaag
     1141 agggccgcga ttttctcacg ttgttctccc tgcatttcga tcacatcgtc tttgatggtc
     1201 ccaccggtgc cgcattcctg ctttagtttc tttgccagag atttcatttc atcgttggaa
     1261 agcaccaaat tcttaaccag ggtcacggct tttccgccgc gcccgctgga ttcgcggtga
     1321 agataaatgg tttgttgttg aggtggcaat gattcccctc tccttccagg agaggggtca
     1381 ggggtgatgt gttttttctt gcgcagatcg ccctgctccg aagaccatac ggttttattc
     1441 tttgacatcg ttcaccaggt tttgtgttga aataatgagt agtgtagagg agaaaatgtt
     1501 gaatgtcaaa tcctttttga aaactgagag ctgcagatag actttgaaag ccatatcgac
     1561 ctacaattcg atacatcaaa ttctcacaaa ggagaacaag atgaatcaaa tcttcgattg
     1621 gtctgtaagt gtgttagtct attgggcaac catttttgga atttcctaca aggaaattaa
     1681 cgtctgggtc ttcctgatcc tctggccgat attgactgcc attctcattg ggattatctt
     1741 gatgcaacac agaaggattc gtaggttatc aaaggaatga tgaaaccatc atgagcaacc
     1801 ttgttgaaat ccgctcttat aacctgaaac caggcgcggg agatgaattt cattatctct
     1861 tccttgaaga ggcgttcccg atgctccagc gctggaatat ggatgttgtc tcctacggtc
     1921 catccttaca tgataaaaac tcatattatc taatacgccg atttgaaact cttgctcatc
     1981 gggaagagag tgaaaatgcc ttttatggaa gtgacgagtg gcgagcaggt ccgtgagaag
     2041 cgatcgtctc tttgattgag aattatgcgg aagttgtgct tgaactggat gatgtcacag
     2101 tgcagatgct gagaaaataa gtatgtccag tttggggcag gggaattgaa tcaatcgttc
     2161 tcgtcactct taactgattc gctcgttagt ggaaactcaa cgatcagcca gggaaaccag
     2221 ggtcccggct ctgcaccggc cgtgtcgagg ttggtaaatt cttcggcacg ttttgcatcc
     2281 tcataggtag cgagcagacc gtgctcgtta aaggtcaacc ccatcgtttg gtactctttc
     2341 tccaaatgat tgcaaagaaa agaatggttg acattaccat attccacgtt aagaagctca
     2401 aagcccagtg ccttgtcaag ctggtcaagt ttctgcggct tgagtaagcg taaatacaaa
     2461 ccggctttgc ccctgccagg gtcaggctcg gatgcctcga ggcattcatc aaaatagtgc
     2521 gttggaatgc caatggccaa taattttatc tgtgggatgg cttgtaaata tcgcttgtaa
     2581 tactcctgtg catcctgcag atttaaccag atgttcggcc agccgaagcg atcttcattg
     2641 aacgcggtat ccacccaagc ctgcatttcc ttgaattgtt catcgctaag agcaaggcgt
     2701 ctctgggtag caaggagttt ttctgctcta gtggataccc agggcaaccc ccatgaatcc
     2761 ggaaagatcg gactgatgca gttgctgatg ctccagatca tggatggaag aaaggtagcg
     2821 ctgtacgagg gtcgacttac accttctacc aggtagtatc caccgagata gtaattcatt
     2881 gatgtccttt cattgaaagg ctggcttatc ctatggacct gtatcgctgt tgtttcagca
     2941 gttggtttta aggcgtttgt gtattgttag gaatcgccgt atcgacgatg atgcagccaa
     3001 ctccatcata catgaaatca ttgaccaccg cgccatccaa cgtgacgagg actctggccg
     3061 ggaaggttat ggtaacggga aggtcacagg tcaacaagga agtattgacc gggttcactg
     3121 cacaggcggt ttcctgatct gaaatttgaa ctgtcaggtc tctatcagtt aagtctggag
     3181 ctgtatctgt gattctaaaa ctgatcaaat ccacagtttg atcgcacatc gttatattgc
     3241 tcgtgagcaa gggcgatgaa ctcgtcggga ctacggcttc cagagtgggt gaagcctcag
     3301 cagcggcggt gacagttgga gatggaaccg agtcgagggt ggcaagaggt gtgtccgtat
     3361 ttacaggcat tgtcgttgcg gtaggggcag aagccatgcc acacagctgt tgatccagtt
     3421 cagccttgga ggctgaatac aattccgaat cgggagaaaa gttttccgcg atcacgggtt
     3481 gtctgactgt tttatcagga caagaataga aacactctgc tcctccaccg ccggggaaaa
     3541 ctgaataatt ttggcagctt aaaccattcg cggaaaccac ggggccgccc tgatcatacg
     3601 ctctacacat ggtgaccaga aacgcagaaa gtgcaagcaa aagatatgta tgtttaggca
     3661 taatcgctga tcattcctat ttcgttaaag atatggatat tccagctatt gtaatattgc
     3721 gtgtaaagat ttgccatcat acctatgggt ggcgaaatca caaaatgtta tactctcacg
     3781 ctgagagtaa tcaaccatgg agcaggatta taaaaaacag gttcatgatc aaatcctaca
     3841 tcaaggtgcc ttcgttcgag gcgtcttcag cggcggtcaa aagggtgcct cgcttccgtg
     3901 ggtcaaagtt ttagtacgcc cggtggagat caagggaatt ctcaacctgc aattctctta
     3961 tttcgacgag aaaaaggata ttaccaagaa ttatgctttg gaagagtcag gggcgaaatt
     4021 agatgagctt cttgctcttc cattccggaa tattttcatc gaaaacacga caggcaatct
     4081 tcagatcaac atatcgaaaa aggggaaagc gctggtaaat cagaagaaaa accctgcctc
     4141 gcatccggct tttgatctgt cccatgatcg ccaaaaaagc aggttgttga ccaccgagaa
     4201 tgcagcgcct ttcttgagag ccgtgggtat catgaccagc gacggcaaga tcagggcgga
     4261 tatgcaaagg aaattcaagc agatcaacga gtttctgcgt ctgttggatg aaacagattc
     4321 atttgatgac ttcaaggaca agcccgttca catcgtggat tttggttgcg gcaacgctta
     4381 tctaacgttc gccgtctact attatcttca cgatgtcttg aatctcgacg ctcacgttgc
     4441 aggggtcgac atcaaagcag acttgatcga acggcatcag gagaaggtaa agtcactcgg
     4501 gtggaaccaa ttgacattcc atgcaggaac aatttcagat tttcaacctg ctgttatccc
     4561 agatgtagtc ctcgctttac atgcctgtga caccgcaacc gacgacgcgc tcgcccgggg
     4621 tattcagtgg gattgcaggt tgatcgtctg tgcgccctgc tgccagcatg aattgcagga
     4681 acagatgtcg catctcccga tgccttcaca actgttaccc atatttcatc atggcatcct
     4741 gttcgagcgt atgggagata tcctcaccga tacgttccgc gcaacagttt tgcggattat
     4801 gggttatcgg acagatgtga cgcaatttgt tcctattgag cacacagcta aaaacctgat
     4861 gatccgcgcc gtaaaaacat caccgccagg taatacacgc tggatcgaag aatataaaaa
     4921 tctcaaagcc ttctggcaag tgacaccata tcttgaacgc ctattgggtg aagcatacat
     4981 tcaattcttc taagaacctg acaatcgaat cattcatcga ggaaaatgcc acatgaaact
     5041 ttccgttaca aatcttggca aacaatatcg acacgacttt tggggattaa aagatttctc
     5101 cctcgaagta gaaccaggaa tccttggcct gctcggaccc aatggcgcgg gcaagtctac
     5161 atttatgcgg atgctggcga cgattaccaa acccactgag ggaaccatca agtggaatga
     5221 agtcgatatc gtcaaatcac cagatgcgat gcgcgcggtg ctcggatatt tacctcagga
     5281 cttcggtgtc tatccaaatt tgaatgcggt tgaattcctt gaatatatgg cagccatcaa
     5341 aggactggat ggcgcctcca cgcggaaacg gattggcgaa ttactgcaag ttgtgaatct
     5401 catggaagcg gcgaagcgtc cgctgggagg ctattcgggc gggatgcggc agcgtgttgg
     5461 aatcgcacaa gctttactga atgatccgca gcttctcatc gtggatgaac cgacggtggg
     5521 actcgacccc gaggagagag tccgcttcag aaacctgctg tcagatctat cgggagaaag
     5581 aatcgtgatc ctgtccacac acattgtttc agatgtggag gcaactgcca cgcatattgt
     5641 cctgctcaac aatggaaaac tcctgcgcca gtctgccccg gaagatctgc ttgccgccct
     5701 ggatggcaag gtctgggaat ggactgtcaa gagcgatgat ctgcctgcgc tcaaaggaaa
     5761 gcacattgtg agcggaacga ttcgacggag cgacggggta caggttcgtg tggtcagtga
     5821 agggaagcct gaagagaacg ctcaaaacac gtcacccaat ctcgaagacg catatctgta
     5881 tttcattggg ggcaaagcat gagcgccgcg cgagtcattt atcacctggc gcgggccgat
     5941 tttctggagc gcgttcgccg ttatagtttc cttatcatgc ttgggctggc cgcctttctc
     6001 agttatcaga ctgctgtagg caacgtcacc atgcggcttg atcagtatcg cggcgagttc
     6061 aattcggcct gggtcgggag catgatggca ctgatttcct cgttctttct cggctggttt
     6121 ggtttttatc ttgtcaaagg ctctgttgca cgcgatcgcg aaacaggtgt cgggcagatc
     6181 atggcaacaa cgccgctgac acgtccgctt tatacatttg gcaagtgggt aagtaacttt
     6241 gccgtactcc tggcgatgaa tatcgtgctt gcccttgcag gtatcggcat tcaatttctt
     6301 gcaggcgaaa atatgcagct caatctggct gctttgcttg caccgtttct ttttattacg
     6361 ctaccagtga tggccctggt ggcggcggtt gctgttctct ttgaaagcat cggctttttg
     6421 agcggaggct ttgggaatgt cgtttatttc ttcctgtttg tttttatctt tccgctggcc
     6481 gataatttga gcaagacaaa ccccgcgctc gagccgttag gcttagggct gattcaacaa
     6541 agtacgagtg tggccgcaaa agctgcattt cccgaatacg atggcggctt tgtcttaggt
     6601 gatccatccg ttgctgaagg actcggattt gacctcgacc ctgcgagtta tatttttcaa
     6661 tggaacggaa ttgactggac gcccgacatc gttatcaagc gtttcgcgct tttcgggctg
     6721 gccgttgtgc tggccctggc agccagcatt ttctttgatc gatttgaccc ttctcgccgc
     6781 cggcctcgca agagaaaagg ctccgcttct ctttcaacgc cggaacctgt caccacatca
     6841 cagtcgttat ctcagcccat ccatttgact ccgttaactg catcgaggaa caacttcgct
     6901 ttcatacgag tgcttatttc ggagttgaaa cttcttctca aaggacagcg ctggtggtgg
     6961 tacgcggtcg ccatcggact attcattgca tccctggtta atacccctga gaacgtacgc
     7021 acctttgtcc ttccatttac atggctgtgg ccgatcctga tctggtctgg gatgggcaat
     7081 cgcgagatcc gccataacgc gcaccagatg gtcttctcct ctgccgcgcc actgttgagg
     7141 cagttgcctg ccacctggct ggctggcttt ctggtgaccg tactgaccgg gagcggcgtg
     7201 gcgttgaaat tattcggtgc aggtgacact gtcggcttgc tggcatggtt ttctgcggcg
     7261 atctttattc cgtcctttgc actggcattg ggcgtgtgga gcaatagtca taaattgttc
     7321 gaggtgttat acgtcacgat gtggtatctc ggtcctttga ataaggtttt tccggtggat
     7381 tatttaggtg cgaacagtga tggcaatatc ggggttttca tcccatgctc gattgcatta
     7441 atcgtgctgg catttattgg cagggcacgg caactccaaa attgaaggct gggtgtgacg
     7501 ctgtatgaat aaaatccccg ggttgataac ctcggggatt tttacttcct atggcacaag
     7561 tcgtgtgtta gtgggaacat tttgattttg aaattagtct tatatgtatc ttcttattcc
     7621 tgcgcacgga actaaaaaat cataatcttg cttttaggag taaacaaatg aaaacaaatg
     7681 gattgtatgc gttcgccatg gagcgacatg ggaatttgtt ccagtcaaag atatttgtgg
     7741 ttgctctcat cctcgcctta gtcgttgctg cctttcctgg aatcaatgcg ttggctgccg
     7801 gtaaccagac tacaatcaca aacgccgggc tggaagatga atggaagagt aaactcaacc
     7861 acctgaaata tcagggactc tacttcgata ctatccggtt ataccctgcc gatttcgatg
     7921 atctttcgga tctggctcag gcgcagtttt atcttgaaaa atatggaatt gcgctaaggc
     7981 aggcacagac cgtcgtcctt aatcacacgg gctttgacat caatggccgc gtgacgaatc
     8041 aggtgcaagc cgctgagact gttcgaaatt tggccatgta cctgcatatg atgcgcgggc
     8101 tacgggataa gatcgaagag gtacccagtc gtaagtaggg tgctgctaca ggcaactcca
     8161 cacttaacat aaaaatcccc gagactttcg tctcggggat tttcgatcat tagtaagtca
     8221 attagccaat cacgacgacg ttgctggcct ggagaccctt ggggccctgt tcaacattga
     8281 actcgacctt ctggccttcc tgcaggttct tgaaaccatc ggcctgaatg gctgaaaagt
     8341 ggacgaagac atcctcgccg ccttcgcgag ccagaaagcc gtagcccttt gttccgttga
     8401 accacttaac tgtaccgttg atacgttccg acatgtttgc ctccttatgg caaagaaaaa
     8461 aatatggagg gtactcattt tgtgttgcat cggaaccgcc tcaacggcga aaaacaaagc
     8521 ggctcacgga cgatccgtga gccaacctgg tacttccaaa gcaaactact ggtatcctac
     8581 ggcgcttaag atatcatgag aattgtcagg tgtcaatgaa attgaatgat gaaattttta
     8641 gtccgagtcc agagccaact gttcggcttt ttcgaactct tccggtgtat tgacattcca
     8701 aaatgccagg ccgagagggt cagcttgaag cgtctcctcg ggagttaatg ttcgcacttt
     8761 tacttgtgga aaccaggaaa tgaccttcca ttgatctgtg tcgatagctg ataagatggc
     8821 cggaagacaa gtctcgcgcc ggtacacagc gtgaaggggc tcgaatcctt cttccgattt
     8881 ggcgatgact acatcggctt cgttctcgag aagcagtctg ctagccattt cgatgagcgc
     8941 cgcgctggcg aagggcatat cacaggcaac cacggcaaca atgggatgcg aggcagatgc
     9001 gatggcggtg tagagtccgc cgagggcacc gcgcccaggc ttgaggtccg cgacgaggcg
     9061 tatcgaagtg ttcagaaatg aataatcctc cggccgattt gtcgtcacaa gggtctcatc
     9121 tgcagtggga gtgagtcggt cgatcacgcg ttgaatgaga ggacgcccca ggaaaagctt
     9181 cagggcttta tcctcgccca tacgggagga ctgtccaccg gcctgaatgg aaattgtgag
     9241 catttgggtg tattataaag gtgagagtag aaggtacgta tggctacgac aattggcaat
     9301 ctgttggaca aattgacagt ggagggggag atctacgaaa aacttgaaga caacacccta
     9361 cgttgttacg catgtggaca tcgttgtttg atacgggaag gcaaacgggg gatatgccag
     9421 gtacgcttca atcaaggtgg agagctgcgc gtaccaagcg gatatgtcgc tgctttgcaa
     9481 agcgaccccg ttgagaagaa accattcttt catgtgctgc caagtacgaa tgcattaacg
     9541 tttgggatgc tcggttgcga tttccattgc ggctattgcc agaattggct gacctcacaa
     9601 gcgatgcgtg acccgtcttc tgatgcggcc ataaataaca ttcgcaagat gactccacag
     9661 gaaatggtgc gtctcgcgca tcggacaaat gcatccgtcg ttgtgtcatc ctataacgag
     9721 ccattgatca cgagtgaatg ggcagtgaag attttcaggg aagcaaaagc agccggtcta
     9781 ctatgcgcgt atgtttcgaa tggaaataac acgccggaag tgatggacta tattcgtcct
     9841 tatatctctg catacaaggt ggacttgaaa tgcatgcagg acaaaaacta tcgcaaactg
     9901 ggtggcgtat tacagaatac actggatgga atcaagcgcg cccatgagat gggtttatgg
     9961 atagaaatcg tgacgcttac tattcccggc ttcaatgatt caaatgaaga actatgggac
    10021 gccgcacgat ttatcgcggg tgtatcgaaa gatattccgt ggcatgtgac agccttccac
    10081 aaagacta
//
LOCUS       DASTJP010000619         8810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_317991, whole genome shotgun sequence.
ACCESSION   DASTJP010000619 DASTJP010000000
VERSION     DASTJP010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317991"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFQ23_18230"
     CDS             complement(<1..369)
                     /locus_tag="VFQ23_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET9908593.1"
                     /translation="MPTLRRFLSILLLSTFILSSCGSPSTPMPATAQPSVPATLTPAP
                     VRGSFETGVYPNLFKDYLGKSDAEIQTKIDSAFDQLFYGDDATERVYYPVGSDMAYIS
                     DIANDDVRSEGMSYGMMIAVQ"
     gene            complement(387..1937)
                     /locus_tag="VFQ23_18235"
     CDS             complement(387..1937)
                     /locus_tag="VFQ23_18235"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HET9908594.1"
                     /translation="MQKTFRNPILPGCYPDPSICRVGEDYYLVTSTFEYFPGLPIFHS
                     RDLVHWEQIGHVLDRPSQLDLDGVRPSGGLYAPTIRHHNGTFYVINTLVDGKTKQGNF
                     VVTATQPQGPWSEPYWLDADGIDPSLFFDEDGKCWYVGTHLNEEGHYKGHTEIFLQEL
                     DYQAMRLIGKVTVLWDGAVKGVVWAEAPHIYKVNGWYYLMIAEGGTAHHHAVTIVRSK
                     NVAGPYETYRGNPVLTHRHLGLDYQIVGTGHADLVETQNGEWWAVLLAMRPYGGYYYN
                     LGRETFLVPVRWEDEFPIFSPGTGRVEFEYPTPDLPEQTWEDLPDRDEFDEPHLALHW
                     NFLRTPRENFYSLQERPGYLRLCLRPQQLTEPGNPSFVGRRQQHMAFTVETELEFTPR
                     STHEYAGLVLIQNNDFHFRFVVTRENTPVIKLIKRSHGQEEVLASQPISADYSILKVM
                     AREQNYNFYFLADGLWHTLAENVDGRILSTPIAGGFVGAYIAMYASSNGRPSTNHADF
                     NWLDYREL"
     gene            complement(1943..2800)
                     /locus_tag="VFQ23_18240"
     CDS             complement(1943..2800)
                     /locus_tag="VFQ23_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9908595.1"
                     /translation="MFATSSPVSWRKSRIITRIFQYLVLSIIVVFVFVPVVMLVFGAL
                     KTRGELLTHPYTPPVPPRWENITGILQTPVFWELLRNSLFVMIVVTVSVVAICSLAAF
                     VFARMEFRTKGLAFNLFTLGLMFPINIAILPVYFVLRQIDLIDNLWGVIIVQTAFQLS
                     GNIMILRGFFTAIPAELQDAAYIDGCTSFGFFWRILLPLARPALSAVAALTMIVSWND
                     FLIPLVVLNSEAFWTLPLGTMQFQGQYGSDLALVSAFITLSAIPTIIFYIFAERQIVA
                     GLTAGAVKG"
     gene            complement(2810..3754)
                     /locus_tag="VFQ23_18245"
     CDS             complement(2810..3754)
                     /locus_tag="VFQ23_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9908596.1"
                     /translation="MQQIPFVQNIFKLNRVSGAPRRFHDNLTIFLFLLPATVLFLVFV
                     VYPILQSVYYSMFNWKGFGPAVDFVGIENYKNILSDKVFLTALKNGLLIILFSLAFQL
                     PLSLLLAVLVGRELPGRVFFRTIFFMPYVLSEVITALMWLFIYNPDPERGFINAVVVF
                     FGGESQTWLASTDLVLPAIFVALTWKYFGFHMLLYMTGLQNIPQEVEEAGRIDGANAF
                     QSFFYITLPLLASTIRTSIYLSVLGSIQQFILVWIMTKGGPVNASETLATYMYRFGFV
                     RFQLGYGSAVAIYMFVLCLMFSIFYQRLTRQPDYLSGL"
     gene            complement(3850..5226)
                     /locus_tag="VFQ23_18250"
     CDS             complement(3850..5226)
                     /locus_tag="VFQ23_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9908597.1"
                     /translation="MSRKIYTILSLLFVAAFVLSACGAPATEAPQTEPEAPAATEPPA
                     AATDAPAATEAPATGEQVTVTWWHITTEEAQKAVWQKLADEYMASHPNVNVEITVLEN
                     EAFKTKLTTVMQSGEPPDIFQSWGGGVMNEYANAGLLKDITADLDANGGEWRNSFSAG
                     ALGVYALKGQNYGVPWDMGMVGFWYNKDLFAQAGIDAPPTTWSELLEDVQALKAAGIT
                     PIAVGEGDKWPGHFWFAYLSTRICGKAGFEAAALRTGSFADPCFVEAGNKILELNALE
                     PFQEGYLAATYGDEATAVGTGKAAMELMGQWAPAVQKDNSEDKQGIGDKLAWFPFPAV
                     EGGAGAPGDAFGGGNGFGIGKNASPEAIDFAKWLTSVEAQMACAEAGFCIPVVKGGEA
                     GLSDPFLITIQETFAQAEYFQLYYDQALPPATGSVVNDSVQGIFAGTLTPEQAAQAIE
                     DSAAAEIK"
     gene            complement(5441..6448)
                     /locus_tag="VFQ23_18255"
     CDS             complement(5441..6448)
                     /locus_tag="VFQ23_18255"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9908598.1"
                     /translation="MRKKKRTVTIQDVAKTAGVSVSTVSRVLNSKVDVASETQDRILS
                     IIDDLGYTTNLAARSMRSQKKNLVGLIMPDIAYPFAIEVMKGVNRAIAESEFDLLVYT
                     TGDVRKSGRAFHEQKYVSLLTNSISDGVIIVAPVAGEFNIDAPIVSIDPLASNPSYPA
                     VHATNYQGAMDAMNYLIGLGHKRIGFISGRAELESSNRRLMGYRDALEKAGLPIDENL
                     IASGDYTTVTGISGARHLLGLEDPPSAIFASNDQMAMGVFQVAEEMGLRIPDDFSVIG
                     FDNITESKYMGLTTIDQFISEMGYVATQMLIKLINDVPLDDQIYRMQTRLVIRNSCRE
                     V"
     gene            6619..8073
                     /locus_tag="VFQ23_18260"
     CDS             6619..8073
                     /locus_tag="VFQ23_18260"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9908599.1"
                     /translation="MATTNGKIYPNLIGGEWRTSDDSTLENRNPADYNEIIGLFPMAT
                     LEDTRIAIKQAGLALPAWARTPAPVRGAILDKASQIISERLDEIARTLTREEGKTLAE
                     AKGEVMRARDIFKYYAGEGWRQGGEVLPNSVNDELLYTRREPLGVVSIITPWNFPIAI
                     PAWKIAPALIYGNTVVFKPAEDAPLTALMMVEALVEAGLPPGVINFLTGDGDLIGTEM
                     TSNPHVNGISFTGSHNTGTRVYQNAIKHMARVQLEMGGKNALVVLKDADITLAVKLAI
                     TGGFGLTGQACTATSRVIAEEEIADEFISALISATKNLVVGNGLENGVQMGPLVNQDQ
                     LQTSQSYMTLGQEEGARLLLGGEVDDGKGYFAQATIFDHVQPTMRIAQEEIFGPVISV
                     MRASGIDDAIEKANAIHFGLSASVVTNNLKLAFAFANQVNAGVVKINAPTTGVALNAP
                     FGGFKHSSANTFKEQGQAAVEFYTRTKTIYVNHG"
     gene            8057..>8810
                     /locus_tag="VFQ23_18265"
     CDS             8057..>8810
                     /locus_tag="VFQ23_18265"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9908600.1"
                     /translation="MSTMDNLFDVQTHAPGPVGRLPLEPDFLTNAPSGHVFGWTQDAA
                     MGWTPSDLGKDEYLILSTQGGIRDSDGSPIALGYHTGHWEVGLLARAAAEEFKEHGAI
                     PFAGFVSDPCDGRTQGTPGMMDSLAYRNDAATVLRRLIRSLPTRRGVLGIATCDKGLP
                     AMLMALAAQRALPCVIVPGGVTLLPEQGEDAGKIQSLGARFAHGLITLEEASDLGCRA
                     CASPGGGCQFLGTAATSQVVAEALGLSVTHSAL"
BASE COUNT         2288 a         2243 c         2282 g         1997 t
ORIGIN      
        1 ctgcacggcg atcatcatgc cataagacat accttcagag cgcacatcat cgttggcaat
       61 gtcggagatg tatgccatat ccgaaccgac aggataatag actcgttctg ttgcgtcatc
      121 gccatagaaa agttgatcaa aggcagagtc gatctttgtc tgaatctcag catcgctttt
      181 tccaagatag tctttaaaca ggtttgggta aacacctgtt tcaaatgagc ctctgacagg
      241 agcaggtgtc aacgtcgctg gaacggaggg ttgtgcagta gcaggcatgg gcgtggaagg
      301 tgatccgcag gaggagagga tgaaagttga aagaagaagg atgctaagaa aacgtcgaag
      361 tgtgggcaag gtttcaattc caataattag agttcacgat agtctaacca attgaagtcg
      421 gcatggtttg tgctgggacg accgttactg ctggcataca tggcaatgta tgcgccgaca
      481 aaaccacctg cgatcggcgt gctcaagatg cgtccatcta cgttctcggc gagggtatgc
      541 cagagaccgt ctgccaaaaa gtaaaaattg tagttctgtt cacgagccat tactttgaga
      601 atggaatagt cagcagagat cggttgcgaa gccagcactt cttcctgccc gtgtgatcgc
      661 ttgataagtt ttattaccgg tgtattttcg cgcgtgacga cgaaccgaaa gtgaaaatca
      721 ttattttgga tgagtaccaa cccagcatat tcgtgcgtgg agcgtggggt aaattccagt
      781 tcggtctcca ccgtaaaagc catgtgctgc tgccgccgcc caacgaaact ggggttgcca
      841 ggctccgtca gttgctgagg gcgcaagcat aagcgcaggt aaccagggcg ttcctggagc
      901 gagtagaagt tctcgcgcgg tgtgcggaga aaattccagt gcagggcgag gtgcggttca
      961 tcaaactcat cccgatcggg caggtcttcc cacgtctgtt caggtaggtc cggggttgga
     1021 tattcaaact ccacgcgtcc cgtgcctggg ctgaaaatgg gaaactcatc ttcccagcgt
     1081 actggcacaa ggaatgtctc acgcccaaga ttgtagtaat aaccgccata gggccgcatt
     1141 gccagcagaa ctgcccacca ttcaccgttt tgcgtttcga ccagatcggc atgcccggtg
     1201 ccaacgatct gataatctaa ccccagatga cgatgggtga ggaccgggtt gccccggtag
     1261 gtttcgtagg gtcccgcaac attcttactg cggacgatcg tcacagcatg gtgatgagcg
     1321 gtcccacctt cggcgatcat caggtagtac catccattca ccttgtaaat gtgcggcgcc
     1381 tccgcccaca ccacaccttt gactgcgcca tcccatagca cggtcacttt gccgatcagt
     1441 ctcatggcct gatagtcaag ttcttgcaga aagatctcgg tatggccttt gtagtgtccc
     1501 tcttcattta gatgtgtgcc gacataccaa cacttgccat cctcatcaaa gaagagagat
     1561 ggatcgattc catccgcgtc aagccaatag ggttcgctcc atggaccttg aggctgggtc
     1621 gcagtgacga cgaaatttcc ctgcttggtc ttcccatcca caagcgtgtt gattacgtaa
     1681 aacgtcccgt tgtgatgtcg gatcgtcggt gcatataaac caccggatgg tctgactcca
     1741 tcaagatcca attgcgatgg gcgatcaaga acgtgaccga tctgttccca gtgcaccagg
     1801 tcgcggctgt gaaagattgg gagacctgga aaatattcaa aggtcgaagt aaccagataa
     1861 taatcttcac caactctgca aatcgaaggg tctggataac aacccggaag gattgggtta
     1921 cgaaatgtct tttgcattca acctaccctt taactgcgcc cgcggtaagc ccggccacga
     1981 tctggcgttc ggcaaaaatg taaaagatga tcgtggggat ggcagaaagt gtaataaacg
     2041 cagaaaccag agcaaggtca gagccatatt ggccctggaa ttgcatggtg ccgagcggga
     2101 gagtccagaa tgcttcgctg ttcaacacta caagcgggat taggaagtca ttccaactga
     2161 caatcatggt cagtgcggca acagcagata acgccgggcg tgccagcggc aatagaatcc
     2221 gccaaaagaa accaaaggag gtacacccgt caatataagc ggcatcctgc aattcagcag
     2281 gaatggcagt aaagaatcca cggaggatca tgatattacc tgataactgg aaggcggttt
     2341 gcacgatgat caccccccat aagttatcga tcaggtctat ctggcgtagt acaaagtaaa
     2401 caggcaggat ggcaatattg atgggaaaca tcaaccccag tgtgaacagg ttgaaggcca
     2461 gtcccttggt acggaattcc atgcgggcaa agacaaaagc cgccaggctg cagatcgcca
     2521 caaccgaaac cgtcacaacg atcatcacaa ataggctatt tctcagcaac tcccagaata
     2581 caggtgtctg taaaattcct gtgatatttt cccagcgtgg tggaacaggc ggcgtgtagg
     2641 ggtgagtcaa taactctccg cgggttttca atgccccaaa gactagcatg acaaccggaa
     2701 caaacacaaa cactacaata atggacaaaa caagatattg aaaaatcctt gtgatgatcc
     2761 ggctctttct ccagctaaca ggggatgatg tagcgaacat ggctgatcct tatagaccgc
     2821 tcaggtaatc tggctggcgc gtcaggcgct gatagaaaat ggaaaacatc aggcagagaa
     2881 cgaacatgta gatcgccacg gcagacccat atccaagctg gaagcgtaca aagccaaagc
     2941 gatacatata ggttgccaga gtctcgctgg cattgacggg tccgcccttg gtcatgatcc
     3001 aaaccaggat gaattgctgg atcgagccga gcacggaaag atagatcgag gtgcgaatgg
     3061 tgctggcgag caatggcaag gtaatataga agaagctttg aaaggcatta gccccatcaa
     3121 tgcgcccagc ttcttcaacc tcctgcggaa tattctgcaa gccggtcata tataacaaca
     3181 tatggaagcc gaaatatttc caggtgagcg ctacaaaaat ggccggcagc accaggtcgg
     3241 tgctggcaag ccaggtttga gattcgccgc caaaaaacac aacgacggcg ttaatgaaac
     3301 cacgttcggg gtccgggttg taaatgaaca accacatcag ggcagtgatc acctctgaaa
     3361 gaacatacgg catgaaaaag atggtgcgga aaaatacccg acccggcagc tcgcgcccta
     3421 ccaacaccgc taagagtaag gacaatggca gctggaaagc caatgaaaaa agaatgatta
     3481 aaagcccatt cttgagggct gtcaaaaaca ccttgtcgct cagaatgttt ttgtagttct
     3541 cgatgcccac aaaatccaca gccgggccaa aacccttcca attgaacata ctatagtaaa
     3601 ccgattggag tattggatag actacaaaaa ccagaaaaag cacagtagcc ggcagaagaa
     3661 ataggaagat tgtcaggtta tcgtgaaatc tgcgcggggc gcctgatacg cgattcaatt
     3721 tgaatatgtt ctgaacaaaa ggaatctgtt gcatgcaagt actcctatct cttcaaaacc
     3781 tatggaggtt gtacgagggt tggttttccc aaccctcgca catggttctc tttactaaga
     3841 gtgattgtgt tactttattt ctgctgcggc cgaatcttcg attgcctggg ccgcctgttc
     3901 aggagtcaat gtacctgcga agataccttg aacgctgtcg ttcacgacag agccagtagc
     3961 cggtggcaga gcctgatcgt aatacaactg gaaatactca gcctgtgcga atgtctcttg
     4021 gatggtgatc aagaacggat cggacaaacc agcttctccg cccttgacaa ccggaataca
     4081 gaaaccggct tcggcacatg ccatctgagc ttccacactg gtcagccact tggcaaagtc
     4141 aatggcttca ggagaggcat tcttgccgat accaaaaccg ttgccgccgc cgaaggcatc
     4201 accaggagca ccagcgccac cctccacagc cgggaagggg aaccaggcca atttatcgcc
     4261 aatgccctgc ttgtcttcac tattgtcttt ctgaacagca ggcgcccact gccccatgag
     4321 ttccatggcg gctttgccag taccaaccgc ggtcgcttcg tcaccatagg tggcggccag
     4381 gtaaccctcc tggaagggtt caagagcatt gagttccagg atcttgttgc ctgcttctac
     4441 gaaacaggga tcagcgaatg agccagtacg taaagccgca gcttcgaagc cagcttttcc
     4501 acaaatgcgt gtggagagat aagcgaacca gaagtggccc ggccatttat cgccctcacc
     4561 tacagcaatg ggtgtaatgc cagcagcctt gagggcctgc acatcttcca atagttcaga
     4621 ccaggttgtg gggggagcat caataccggc ctgtgcgaag aggtctttgt tgtaccagaa
     4681 accgaccatg cccatatccc acggtacacc gtagttctgg cccttgaggg catacacacc
     4741 caatgcacca gcagagaagg agttgcgcca ttcgccgcca ttggcatcca ggtccgcagt
     4801 gatatctttg agcaaaccgg cattggcata ttcattcatt acaccgccgc cccagctttg
     4861 gaagatgtct ggtggctcac cagattgcat cacggtggtc aacttggttt tgaaggcttc
     4921 attttccaaa acggtgatct caacattcac gttcggatgt gaagccatat attcatcagc
     4981 aagtttttgc cagacggcct tttgggcttc ttcggttgtg atatgccacc aggttacggt
     5041 aacttgctct cccgtagctg gtgcttctgt ggcagccggt gcgtctgtag ctgcagctgg
     5101 tggttcggtg gcagcgggcg cttcgggttc agtctgggga gcttcggtcg ctggtgcgcc
     5161 acaggcagaa agtacaaaag ccgctacaaa caaaagactc aaaatggtat aaatcttacg
     5221 agacattttc ttctcctttg aggttctaaa caggttatac tagcggcgtg cggagataac
     5281 cctccacacg tgtccgcagg tcttccttcc gctgtccaag gtcgaagaga agacctgcaa
     5341 tttcctttca aacaactgac tcgctcacct cctttcgatt tccaatacca tatccaaaag
     5401 caattgcaga gttgttgtga tcaaaacttg ttaattcaca tcaaacctcg cggcaggaat
     5461 tacgaatgac aagccgggtt tgcattcgat agatttggtc atccagcggc acatcattga
     5521 taagcttgat caacatttgc gtggcaacat atcccatttc agaaataaac tgatcaattg
     5581 tcgtcagccc catgtatttg gattctgtga tgttgtcaaa accaatcaca gagaagtcat
     5641 ctggaattcg caatcccatt tcctcggcaa cctgaaaaac acccattgcc atttgatcat
     5701 tggatgcaaa aatggcggac ggagggtcct ccaaaccaag caaatggcgt gccccactga
     5761 tgccggtcac tgtggtgtaa tcaccagaag cgatcaggtt ctcatcaatg ggaagccctg
     5821 ctttttccag cgcatcgcga tatcccatca gacggcgatt tgaactttcc agttcagcac
     5881 gcccactgat aaagccaata cgcttatgcc ctaagccgat aagataattc attgcatcca
     5941 tcgcaccctg atagttcgtg gcgtggactg ccggataact tgggttgctg gcaagtgggt
     6001 caatggaaac aatcggtgca tcgatattga attcacccgc taccggagca acgatgatca
     6061 ctccatcaga gattgaattt gtcaggaggg agacatactt ctgttcgtga aaggcgcgtc
     6121 cgcttttgcg cacatcgccg gttgtataca ccaacaggtc aaactcagat tctgcgatgg
     6181 cgcggttgac tcccttcatc acctcgatcg caaaaggata ggctatatcg ggcatgatga
     6241 gcccaaccag attctttttc tggctgcgca tactgcgagc tgccaggttg gttgtataac
     6301 ccaaatcatc aataatggag aggatgcggt cctgtgtttc actcgcgacg tccaccttgc
     6361 tgttgagcac gcgcgaaacc gtcgaaacag atacccccgc ggtcttggct acatcctgaa
     6421 ttgtcaccgt tctttttttc ttacgcatac caatttccta ttcaagaatg atagaatagc
     6481 ggtatcgtta ccggtaacga taccgctatt atggcatatg ttagagttaa agtcaactta
     6541 atttaaggtt tacccattcg ggtaccatag ggtaatcttt tctctagtaa aaacaggcaa
     6601 agatggaggc tactgagtat ggcaacgaca aatggaaaaa tctaccctaa tctgatcggc
     6661 ggggagtgga gaacgtccga tgatagcacg ctggagaacc gcaatccagc cgactacaac
     6721 gaaatcatcg ggctcttccc gatggcaacg ctggaggata cgaggattgc gatcaaacag
     6781 gcaggtttgg ctttgcctgc ctgggcgaga acgcctgccc ctgtgcgtgg cgcgatcctt
     6841 gataaggcca gccagatcat ctcagaacgg ctcgatgaaa tcgcgagaac tctgacccgc
     6901 gaagaaggca aaactcttgc cgaggcaaaa ggagaggtga tgcgtgcccg cgatattttt
     6961 aagtattatg cgggcgaagg ctggcgccag ggtggtgagg ttctccccaa ctctgtcaac
     7021 gatgaactgc tttacacgcg tcgtgagcct ttgggtgtcg tcagtattat cacaccgtgg
     7081 aattttccga tcgcgatccc tgcctggaag atcgcgcccg cgcttatcta tggaaacacc
     7141 gtcgtcttca agccagctga agatgcacct ctcacagccc tgatgatggt tgaagctctc
     7201 gtagaagcgg gcctgccccc gggcgttatc aacttcctga cgggcgatgg ggatttgatc
     7261 ggcaccgaaa tgacaagcaa ccctcatgta aacggaatca gttttacggg ttctcacaat
     7321 acaggcacga gggtctatca gaacgcgatc aagcatatgg cgcgcgttca attggaaatg
     7381 ggtggcaaaa acgcgctggt cgttttgaag gatgcggata taacgctcgc tgtcaaactg
     7441 gctatcaccg gcggcttcgg gcttacgggc caggcctgta cggcgaccag tcgcgtgatt
     7501 gcagaagaag aaattgcaga tgagtttatc tccgcgttga tcagcgccac gaaaaatctg
     7561 gttgtcggca acgggctcga aaacggcgtc cagatggggc cactggttaa tcaggatcaa
     7621 ctgcaaacca gtcaatctta tatgacgctt ggtcaagaag aaggtgcaag gttattgctt
     7681 ggcggtgaag tagatgacgg aaaaggatac tttgcacagg ccaccatctt cgaccatgtt
     7741 caaccaacga tgcgcatcgc gcaggaagag atttttggtc cggttatcag cgtgatgcgt
     7801 gccagcggca tcgatgatgc cattgaaaaa gcaaatgcga ttcattttgg actttctgca
     7861 agcgtagtaa ccaacaatct aaagcttgcc tttgcgtttg cgaatcaggt aaacgcaggt
     7921 gttgtgaaga tcaacgcacc cacaactggc gttgctctga atgcaccctt tggcgggttc
     7981 aaacattcca gtgccaatac cttcaaggaa caagggcaag ctgccgtcga attttatacc
     8041 cgtacaaaaa cgatctatgt caaccatgga taatctcttt gatgttcaga ctcacgcacc
     8101 gggcccggtt ggacggttgc ctctcgaacc ggatttctta accaacgccc caagcgggca
     8161 cgtcttcggc tggacgcagg acgcggccat gggatggact ccgtccgacc ttggtaagga
     8221 tgaatatctg atccttagta cgcaaggcgg catccgtgac tccgacggct cccccattgc
     8281 gctggggtat cacaccggac actgggaagt cggtttgctt gcccgggcag cggcagaaga
     8341 attcaaagag cacggtgcga ttccatttgc aggctttgtc agtgatccct gcgatggacg
     8401 aacacaggga acgccgggca tgatggacag tctcgcctat cgcaacgacg cggcgaccgt
     8461 gctgcgcaga ttgatccgct cgttgcccac gcgacgcggc gtccttggca tcgcgacctg
     8521 cgataaaggc ctgcccgcca tgctgatggc actcgccgca caaagagcat taccatgtgt
     8581 catcgtccct ggcggcgtga ccctgctccc ggaacagggc gaagatgcag ggaagatcca
     8641 gtcactggga gcgcgctttg cacacgggct cattacattg gaagaagcct ccgacctggg
     8701 atgccgcgcc tgcgcctcac ccggaggcgg ctgtcagttc cttgggactg cggcgaccag
     8761 tcaggttgtc gcagaggcat tggggttatc ggtcacacat tctgcactgg
//
LOCUS       DASTJP010000620         6914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1211850, whole genome shotgun sequence.
ACCESSION   DASTJP010000620 DASTJP010000000
VERSION     DASTJP010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211850"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            560..1375
                     /gene="surE"
                     /locus_tag="VFQ23_18270"
     CDS             560..1375
                     /gene="surE"
                     /locus_tag="VFQ23_18270"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET9908601.1"
                     /translation="MSNPRLQILLTNDDGIGSPGLWAAAAALSEIGFVTVTAPRDQSS
                     GMGRSMPNTSDGIIEKKQVEVNGREWSVFSVGGSPAQAVLHGVYEVMKRKPDLVVSGI
                     NYGENVASGITISGTVGAAMEAASLGIPSIAVSLEAHSKYHLSYSDEMNFSTAAVFTA
                     RFAHILLQSKFPPDVNFLKVDVPADATIDTPWQLTRMSLQRYYEPIQPKRESWNEPAR
                     LEYQEAGVLDGEAEDTDVFVLRKKRFVSVTPMSLDLTSRVDFVELEKLMRRQK"
     gene            complement(1495..1821)
                     /gene="trxA"
                     /locus_tag="VFQ23_18275"
     CDS             complement(1495..1821)
                     /gene="trxA"
                     /locus_tag="VFQ23_18275"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9908602.1"
                     /translation="MAEMLHVTEDTFKTEVLDSTQPVLVDFSAVWCQPCKMLDPIVKQ
                     LAGEWDGKVKVVKIDADENPNLVMQFGVMGIPTLLFMKGGEIKERITGFMPKDKLIAR
                     FSPHFN"
     gene            1914..1985
                     /locus_tag="VFQ23_18280"
     tRNA            1914..1985
                     /locus_tag="VFQ23_18280"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1946..1948,aa:Lys,seq:ttt)
     gene            2292..2978
                     /locus_tag="VFQ23_18285"
     CDS             2292..2978
                     /locus_tag="VFQ23_18285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908603.1"
                     /translation="MEKKIRVTILDDHQSIVDGYRFRLSKIPQIEVIDALQHGEDLEP
                     SLRKQVPDVLLLDVYVPVSVENRNPYPILRIIPDLVQRYPALEILVISMHAERGIIRA
                     VMQSGASGYILKDDQKTIQDLGNVILSIARGGIHLSDKAHAAYIRNHNVVSEEPLTGR
                     QMKVLSLCAAYPDHSTSDLAREMNVAHSTIRNLLSEMYIRLGVHSRAAAIAKARQLGL
                     IAPDSPAMPI"
     gene            3711..5981
                     /locus_tag="VFQ23_18290"
     CDS             3711..5981
                     /locus_tag="VFQ23_18290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9908604.1"
                     /translation="MNTWKKILWLIIERMVPWLVLAILLFYTYAKFFGHPYGFRWGSS
                     EGVIRYVFIEQPEPTLQENDRIVQIGDVTWEEFREDLRRTFFDGAKPGDLVPITVLRD
                     DERIDISWVYPGFNWNEFQEQFFSEWGMSYVLWMAGLLTILLIRPKDDRWLLMILFNF
                     LTAIWLTAGSGVSSFQVWISAVVLRMAIWLSIPVYLHLHWVFPQPLGKLSKKVIGLVY
                     TGALVLAIAQWFQLLPRDSYLLGFLVALAGSFILLVLHIVRQPSARRALRLPIVVAFS
                     SLILAVTWQIMYSRNLIHTWFGSVGLLGVPLIPLAYLYSAFRRRLGDLELRVNRFFSI
                     YLFVILLGIVELSLIFLLSQTLQFSGEVLTISLISAAVAAIAFIWIYPAFERFVERRI
                     FGIPLPSKNLLEIFSNRITTSNSLPDLVRVIDEEVIPSLLIRQFAFLHEENGSFRVLS
                     KPGLSEGQITNVPVVNELMKETMTPRSSDPLSENDPYSWIRLILPLRLGEETIGYWLF
                     GRRDPDDLYSQLEIPIIKSLANQAAVALSNILQTERIKAMNEANIDRFEQEKLRLSRD
                     LHDTILNQIATLLMSSDAPVFSPAFQAGFEELTTRLREIVSDLRSPMLDLGLKLAFED
                     LADKLAERNQDTVSVVAAIQTDGEYRYPEKVESHLYRIVQEACENALKHARAKSIKLT
                     VRLFRDRIELQVEDDGVGFDTGTSLKLDSMIKDKHFGLAGIYERSSLIGAQVYVDSKP
                     GQGTRVRIIWESKSSI"
     gene            6015..6512
                     /locus_tag="VFQ23_18295"
     CDS             6015..6512
                     /locus_tag="VFQ23_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908605.1"
                     /translation="MDMGGSLAIPLLIGVPVNTKDNDYWKQQTGVDPRLVCLYNYQGA
                     GIGFNLGYFLIFPLNLARHYLILESAGWVAIAVATVGLILGNRSARVVPYNLWIPYGR
                     LTLSYGAMFFVMALLGPMWGLFFLEYYSFFRNPLWGSLVILIAAIMGRIITVRQSRRQ
                     ILAKQ"
     gene            6588..>6914
                     /locus_tag="VFQ23_18300"
     CDS             6588..>6914
                     /locus_tag="VFQ23_18300"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9908606.1"
                     /translation="MSFAHLHVHTEYSMLDGFSNIKKLVKRVKELEMPAVAITDHGTM
                     FGVIDFYKAATSEGIKPIIGVEAYLSARGMADRDSKLDRTSSHLLLLAENETGYKNLL
                     KISSAAQ"
BASE COUNT         1712 a         1589 c         1655 g         1958 t
ORIGIN      
        1 tatccctcga ccttgaaacc acggcaagcg attttatccc gctcagtggt gactaataca
       61 aaaaaggagt tctagattga cgaaaactaa gaaagtaatt gtagaaccga ctgatcagtc
      121 ggaccttgag gcaatgccat cgactgaaga gaaaacaccc gttgtcgatc aatactcggc
      181 agatgcagta gctttttggc gggaatatct cagtacgccc cgcagaaaaa gaccccctgt
      241 aaagcttacg aaattatcat agcctgcaga aatacaggta ccgagtcgct tcgtcctctt
      301 tggtgattcg tgatcctccc ccagaccgtc atagctgggg gttgtatttt gagtgcgttg
      361 ggcggggtgt cgcttcggag agagtcgggt ttgtatcttg gtgaccggca gaacttgaaa
      421 aaaccttgac gcattttact gcgcgcgctt acactttcat gtacggttgc ttgcggagca
      481 actggtcgtg ccaagggcgc catttttgat gagcggatca accgctcctt tttatttact
      541 cacctctgtc aataggaata tgtcaaatcc aagattgcaa atccttctta ccaatgatga
      601 cggcatcggc tcaccgggct tgtgggctgc tgctgctgcc ctttcagaga tcgggttcgt
      661 tactgtcact gccccgcgag accagtcttc gggcatgggg cgcagtatgc ccaacacatc
      721 agatgggatc attgaaaaga aacaggtaga ggtcaacggt cgtgaatgga gtgtattttc
      781 tgtggggggg agtccagctc aagcggtcct gcatggtgtt tatgaagtca tgaagcgaaa
      841 acctgacctg gttgtatcgg gcattaatta tggcgaaaat gtggcctcgg gtatcaccat
      901 ttcagggact gtgggcgcgg cgatggaagc tgcttctttg ggcatccctt ccatagcggt
      961 ttccctggaa gcccattcaa agtatcacct ttcttattcg gacgaaatga atttctccac
     1021 cgcagcggta tttacagcac gctttgctca tattctgttg caaagtaaat ttcctcccga
     1081 cgtaaatttt ctaaaagtgg atgtacctgc ggacgcaacg atagataccc cctggcagtt
     1141 gacacgtatg tctctgcaaa gatattacga acctattcag ccaaaacgtg aatcgtggaa
     1201 cgaacctgcc aggcttgaat atcaggaagc cggagtgttg gacggggaag ccgaggatac
     1261 agatgtattt gttttgagaa agaaacgatt tgtgtctgtt actcccatga gcctggattt
     1321 aacgtcccgc gtggactttg tagagctgga gaagttgatg agaagacaaa aataatggac
     1381 acgctagggt gtccattatt tttgttaggc gttggcattg cgagcccgag cagagcgagt
     1441 ggcgaagcaa tctccatctt catcggattg ctcaccagca ctgcaccgag cctgttaatt
     1501 aaagtgaggg ctaaaccgtg cgatcagctt atcctttggc ataaagcctg tgatccgttc
     1561 cttgatctcg ccgcccttca tgaacaacaa tgtgggaata cccattacac cgaactgcat
     1621 gaccagattc gggttttcat ccgcatcgat ttttacaact ttgaccttgc catcccactc
     1681 cccagccagt tgcttgacaa tgggatccag cattttacag ggctgacacc acaccgcgct
     1741 aaaatctacc agtactggtt gagttgaatc taacacttcg gttttgaatg tatcttctgt
     1801 gacgtgcaac atttcagcca tttgagcctc catataaaag acgtattgta cttttcaggt
     1861 cttacgttga cgctgaatat ttaccatgtt agaatgcttt tcgcctatgc ttcgggcgcg
     1921 tagctcaatg gtagagcagt ggccttttaa gccattggtt ctcggttcga atccgagcgc
     1981 gctcactagg acgatttagt cgtaacggtg ttataagcat ccttcccaca gaagatactt
     2041 atcgcaccgt tattaatact aaaagtgaat gtcgaaggtg tttcgacatt cacttcaaaa
     2101 taactccttc cccctttgca tcccacttcc aagcatacga ataattactt ctaacattgt
     2161 atttttacgg gtaacattgc tcaagaagca actgattatt tcaggtagac atttgtactg
     2221 attcttcgta aaaaaccttg aaaatttatg taaatgttgc tacccttgaa taggcggaac
     2281 caggaggcga aatggaaaag aaaattcgcg taacgattct ggatgaccat caaagcattg
     2341 tggatggcta tcgattccga ttgagcaaaa ttccccagat tgaagtaata gatgccctgc
     2401 agcatggtga agatctagag ccttcattga gaaaacaggt tccagatgtt ctccttctcg
     2461 acgtatatgt tcctgtctct gtagagaacc gaaaccccta ccccattctt cgaattattc
     2521 ctgatctggt ccagcgctat cctgctcttg aaattctggt gatcagtatg catgcagagc
     2581 gcgggatcat ccgcgcagtg atgcagagtg gtgcaagcgg atatattctt aaagatgatc
     2641 aaaagaccat ccaagatctg ggaaatgtaa ttctctcgat agcccgcggg ggaatccatc
     2701 taagcgacaa ggcgcacgcc gcgtacatca ggaatcacaa tgttgtcagc gaagagccct
     2761 taaccggccg acaaatgaaa gtgctttccc tttgtgcagc ctacccggac cattcaacca
     2821 gtgatctggc acgcgagatg aatgtagcac attcaaccat ccgcaatctg ctctctgaaa
     2881 tgtatatacg gctcggagta catagccgcg ctgcagcaat tgctaaagcg cgccagttgg
     2941 ggctgatcgc tccagattcc cccgccatgc ctatttgatc aaattttgag acgaaattcc
     3001 cagcgtgtcg aagagccggt gaacgggagc gatcacggct tcaataacgg aatcaatcat
     3061 ccctctgttt tgaagtggga tattgtctaa aatagtttgg gcggcttggt gtctcattag
     3121 aatttgatcc acacaatagc tgaccgcccc gcaatggatt aaaatatcct gagcttcccg
     3181 taaaaccccc tcttcagata tatttcgata cagttccata aatctttctc gattaggacg
     3241 gtccacagtt tgtgcaaaca ggatggaaac gcacagccat aaaatggcgc gctcttattt
     3301 ttcaccacct gccagtagga tatttcatct gtgggatttt gaacatccaa ctcctgacca
     3361 gctcattgtg gattgtcgct atgcagcact agtcttttga ttctgttatt cccattcctg
     3421 ccagccgaga tcgctcttca gagtgttggc ttgtttgtgc tcgatcgggg agatcttggc
     3481 gagagcctcc aggtagggat ccctggacca gtgacagaag agatcgctgc atagaagctt
     3541 ttcatggtat tcatttggat tactccttta atcttgggtt tttggtacgt accttgagtt
     3601 gaatagacgg gtttttctat ttggtacctc cttttttagg atctctaacg ctcaacaatc
     3661 agtcgcgacc gccaacgagg aaaatatctg ataaaaatgt ctgacagaat atgaacacct
     3721 ggaaaaaaat tctctggttg attattgaac ggatggtgcc atggcttgtc ctggcaatct
     3781 tactttttta cacgtatgcc aagttttttg gtcatccgta cggttttcgc tggggttcgt
     3841 cagaaggtgt tattcgttat gtctttattg aacaaccgga accaacactt caggaaaacg
     3901 atcgaattgt tcaaataggg gatgtcacct gggaggaatt cagggaggat ttgagaagaa
     3961 cattcttcga tggagcgaag cccggggatc ttgtgccaat cactgtcctg cgcgacgacg
     4021 aaagaatcga tatttcctgg gtatatcctg gatttaattg gaatgaattt caggaacagt
     4081 tttttagtga atggggaatg tcatatgttt tatggatggc aggattattg acgatccttc
     4141 tcatccgccc gaaagatgac cgctggctgc tcatgatcct ctttaatttt ttaacggcta
     4201 tttggctaac tgccgggagt ggtgtttctt catttcaagt ttggatcagc gcagtggtat
     4261 tgcgtatggc tatctggtta agcattcctg tttaccttca tttacattgg gtatttccgc
     4321 agccacttgg aaaactgtcg aagaaagtga tcggattggt ttacactggc gcactggttc
     4381 ttgctattgc gcagtggttt caacttttgc cgagggattc ttatcttttg gggtttctcg
     4441 tggcactggc gggaagcttc attttgctag tcctccatat tgtgcgccag ccttccgcgc
     4501 gacgtgctct tcgtcttccc attgtagtag ccttctcgag cttaattctg gcggttactt
     4561 ggcaaattat gtacagtcga aatctgattc acacctggtt tggaagtgtg ggtttgctgg
     4621 gtgttccact cattccactc gcttatcttt actccgcatt ccgccgtcgt ttaggtgatt
     4681 tggaactgcg cgtgaatcgc tttttctcga tttacctttt tgtgattttg cttggtatcg
     4741 ttgaactttc tctaattttt ttactaagtc agacacttca attctctggc gaagtactaa
     4801 ccattagcct gatatctgct gctgtagcag cgattgcctt catctggatc tatcctgctt
     4861 tcgaaaggtt tgtcgaacga cgtatttttg gcatcccctt gccatccaaa aatctgctgg
     4921 agattttttc gaatcgcatt accacaagca attcactgcc tgatctggtc cgtgtcattg
     4981 acgaagaggt tattcccagc cttctgatcc gtcagtttgc ctttcttcat gaggagaatg
     5041 gttcattccg agtgctctca aaaccggggt tgagcgaagg acaaatcacg aatgtgccag
     5101 tcgtgaacga attaatgaag gaaacgatga caccgcgctc atctgatccg ttgagcgaga
     5161 atgatcccta ttcctggatt cgtctcattc tgcctctcag gcttggcgaa gagacgatcg
     5221 gctattggct ctttgggcgg cgtgacccag acgatttgta ttctcaattg gagattccaa
     5281 ttatcaagtc cctggcgaat caggcggccg tggcgctaag caatattctg cagacggaac
     5341 gcatcaaagc gatgaatgaa gcgaatattg accgctttga acaggaaaaa cttcgtcttt
     5401 cacgtgactt gcacgacacc atcctcaacc agatcgcgac cttgttaatg agttcagatg
     5461 cccctgtctt ttcgccggct tttcaggcag gctttgaaga actgacgaca agattgcggg
     5521 aaattgtcag tgacttacgg tcccctatgt tagatttggg actgaaactg gcctttgagg
     5581 accttgcaga caaattggca gaaagaaatc aggatactgt gagcgttgta gcagctattc
     5641 agacggacgg cgaatacaga tatcctgaaa aagtcgagag ccatttatat cgtattgtcc
     5701 aggaagcctg tgagaatgct ctaaaacacg cgcgcgcaaa gtcaattaag ctcaccgtac
     5761 ggctctttcg cgataggatc gagcttcagg tggaagatga tggagtcggt tttgataccg
     5821 gcaccagcct caaattggat tccatgatca aagataaaca ttttggcctg gccgggatct
     5881 atgaacgctc gagtttgata ggcgcacagg tctatgttga ctcgaagcca ggtcagggca
     5941 cgcgggtacg gattatttgg gagtctaaat cctcgatttg attgttaagg ttcaggatat
     6001 aaaaagtcat actgatggac atgggcggat cgctggcaat tccccttctt atcggggtac
     6061 ccgtcaatac caaggataac gattattgga aacagcaaac gggagtagat ccacgtcttg
     6121 tgtgcctata taactatcaa ggtgccggga ttggatttaa cctgggttac ttccttattt
     6181 ttcctttaaa cctggcgcgc cattatctaa tccttgagtc ggcgggctgg gttgcaattg
     6241 ccgttgctac agtagggctg attctgggta acaggtctgc gcgtgttgtt ccgtacaact
     6301 tatggatccc ctacgggcga ttaaccctat cttacggagc aatgttcttc gtaatggcat
     6361 tgctaggtcc gatgtgggga ttgtttttcc ttgagtatta ttcatttttt cgaaacccat
     6421 tatggggatc actagtgatt ttgatagctg ctattatggg aaggataatt actgtgcggc
     6481 aatcacgcag gcagattctt gcaaagcaat aactattgtc tgttccgaat attttaagcc
     6541 tcccgccaac tttatagatg aacatgcgtg ctataatcct ttagcccatg tccttcgcac
     6601 atctccacgt tcacaccgaa tattccatgc tggatggctt ctcgaacatt aagaagctcg
     6661 tcaagcgtgt aaaggaattg gagatgccgg ccgtggcgat cacggatcac ggcaccatgt
     6721 tcggggtcat cgatttttac aaagccgcta cgagcgaagg gatcaagcca atcatcgggg
     6781 tggaagctta tctttcggcg cgcggcatgg cagaccgcga ctctaagctt gaccggacgt
     6841 cctcgcacct gctactgctc gcggaaaatg agacgggcta taaaaacctg ctcaagatct
     6901 cctcagcagc acag
//
LOCUS       DASTJP010000621         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_403842, whole genome shotgun sequence.
ACCESSION   DASTJP010000621 DASTJP010000000
VERSION     DASTJP010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403842"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..>2507)
                     /locus_tag="VFQ23_18305"
     CDS             complement(48..>2507)
                     /locus_tag="VFQ23_18305"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908607.1"
                     /translation="FIYLRLPALLLVLYVLLIPSPSISKIRVQGNVGYIAEGYDFTIL
                     NLTSTGQITPISSYTLPYWIRDFTIENGIAYVLTIDNSIHIVDTHQPIDNSKLAHWNS
                     PGLLHSVEVEGNFAFLANHSEGVRVFNIQDPINPYQILHKSDIGTVLDVAATQNLIYV
                     ARGPDGLDIYDISTITKPELKGHYDAGSSINKVVLLPDGRALLQVDKQGLQIINLANP
                     QNIILTNTFTTDKDIVRTIVNNNRIFVAQKRAGILIVRIKEDNTLESLTTIPAPRNAL
                     DAALIENTIFVAAGWRGLQALDISKIEDNHQINEYSDRFWKYKAFIGFLVVASLLFWL
                     AFFAQFVLPVRTFVQRQMIFNRLILFLIGRHGPAIFIENGRAREQIGERQRKGPGVLW
                     LDTASATVIRTATRFKEAIGPGVHFTKKNEFITGTVDLHTQVDVFGPQDDENPFMAKP
                     GTAAEQADLAKYSEIQKRRMEVSALTRDGIEVVPKISITFRVDTLPSEDGQPGSRFGY
                     RTGTSKEDKANEASDKEAIYKAIAGEGINKYRSKHRVAWNQLPLLLAVDVWREYVAKF
                     TLEELYDSQDVAFLQPALPASVDVKIDQSEVVDQNGVDPGHFQQKNTSFLHDLNKQIE
                     SWLDRVENKNVKKDAEQKAESSASKSNETMNKTALQIINEMVKARLTFEYVQKINSIG
                     KREPGLVESNEYKILKARGLRVLSVSISNPRVNEIVEKQRIDQWKASWLSNANAERDR
                     IDRQRGFLEVQGKENAANEYAHDLARHIMKQKPQSFKEIVKALLMRTRLITIQKDRLQ
                     RRMGTEREDLEDILKWLEESP"
BASE COUNT          653 a          586 c          503 g          765 t
ORIGIN      
        1 gcgctgctcg tttcgatctt ggttttcaga agccgatgtc agaactgtca tgggctttcc
       61 tccaaccact taagaatgtc ttcgagatcc tcacgttcgg tccccattcg gcgttgtagc
      121 cggtcttttt ggatcgtaat gagacgggtc cgcatgagca gagcctttac tatctctttg
      181 aaactctgcg gtttctgttt cataatatga cgcgcgaggt cgtgagcata ctcatttgca
      241 gcgttttctt ttccttgaac ttccaggaaa ccgcgctgtc gatcaatgcg atcacgctca
      301 gcatttgcat tgctgagcca ggatgccttc cattgatcaa tgcgttgttt ttccacgatt
      361 tcattcacgc ggggattgct gatactaaca cttaacactc ttagtccacg cgctttcagg
      421 attttatatt cattgctttc aacaagcccc ggctctcgtt taccgatcga atttatcttt
      481 tgaacatact caaacgtcag acgcgccttg accatttcat tgataatctg taaagccgtc
      541 ttattcatag tttcatttga cttagacgca gaagattcgg ctttttgttc tgcgtctttc
      601 tttacattct tattctcaac acgatccagc caggactcta tttgcttgtt taaatcatgc
      661 aggaatgagg tatttttctg ctgaaaatgt ccgggatcta caccattctg atccactact
      721 tcagactgat ctatctttac atcgactgat gctggcaggg cgggctgtaa gaaggcgacg
      781 tcctgagaat catataattc ctccagtgta aacttggcga catattcccg ccatacatcc
      841 acagcaagca gcaggggcaa ctgattccag gcgactcggt gttttgatct gtatttattg
      901 attccttcgc ccgcaatcgc tttgtaaatc gcttccttat cgcttgcttc attcgcttta
      961 tcttctttac tagtccctgt tcgataacca aatcttgaac cgggctggcc gtcctctgat
     1021 ggaagtgtgt ccacccggaa agtaatcgag atttttggaa ccacctcaat accatcacga
     1081 gtcaaagcac tgacttccat gcgtcttttt tggatttcac tatactttgc cagatcagct
     1141 tgttctgccg cagtacctgg cttggccata aatggatttt catcatcttg cggaccaaac
     1201 acatcaactt gtgtgtgcaa atccacggta ccagtaataa attcgttctt ttttgtgaaa
     1261 tgaacaccag ggccaattgc ttccttaaac cttgtcgctg ttcgaatgac tgtagcactt
     1321 gcggtatcta gccacaagac accaggacct tttctttggc gttcaccaat ttgttcacgt
     1381 gcgcgcccat tttcaatgaa aatcgcaggc ccatgcctgc cgatcaaaaa taaaatgagt
     1441 ctattaaata tcatttgacg ctgaacaaag gtccttacag gcagaacaaa ttgcgcaaag
     1501 aaggctagcc agaaaagcag ggaagcaaca acgagaaaac caatgaatgc cttatatttc
     1561 cagaatctgt cagagtattc gttaatttgg tgattatcct caatttttga aatgtcaagg
     1621 gcttgtaatc ctcgccagcc ggcggcaaca aaaatggtgt tttcaatgag tgctgcatcc
     1681 agtgcatttc gtggcgcggg aatagttgtg agactttcaa gagtattgtc ctccttaatt
     1741 ctaacgatca gtatccctgc tcttttttga gcaacaaaga tcctgttgtt atttacaatc
     1801 gtcctgacaa tatccttatc tgttgtaaac gtattcgtca ggataatatt ctgcggatta
     1861 gctaaattta tgatttgtag gccctgctta tccacctgca aaagggctct gccatcagga
     1921 agcaacacca ccttattgat agaactacct gcatcatagt gacctttaag ttctggttta
     1981 gttatggtgc tgatgtcata gatatccaaa ccatcaggtc cccgtgcaac atatatcaag
     2041 ttttgcgtcg cggctacatc caacactgtt ccgatatcac tcttatgcag aatttgataa
     2101 gggttaatcg ggtcctggat attaaagact cgcacaccct cagaatgatt tgctaaaaat
     2161 gcaaaatttc cctcaacttc aacagaatgc agcagtcctg gtgaattcca atgggctaac
     2221 ttgctgttgt caatgggttg gtgagtatcg acaatatgaa tgctattatc tatggttaag
     2281 acgtaagcta ttccattctc aatcgtaaag tctctaatcc aataaggcaa cgtgtaggag
     2341 gagattggtg taatttgacc cgttgaagtc aaatttaaaa tcgtaaaatc atatccttcc
     2401 gcaatatatc ccacattacc ctgcacgcga atcttgctga tagacgggga tggaataagt
     2461 aagacataca acaccaggag gagtgctgga agcctcaagt atataaa
//
LOCUS       DASTJP010000622         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_241161, whole genome shotgun sequence.
ACCESSION   DASTJP010000622 DASTJP010000000
VERSION     DASTJP010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241161"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFQ23_18310"
     CDS             <1..267
                     /locus_tag="VFQ23_18310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9908608.1"
                     /translation="LLEGMACGCFPVAGDLESIREWITPNANGLLFDSNDPGSIATAI
                     VDAIRNEELRNKAVELNQKIITTRAEYQQNMKHAEEFYKIVCNL"
     gene            280..1071
                     /locus_tag="VFQ23_18315"
     CDS             280..1071
                     /locus_tag="VFQ23_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9908609.1"
                     /translation="MDLGLKNKRAFLAGSSRGLGFATALTLASEGCEVAINSRDEEKV
                     KAAAEEISSKTGTRAFGMTGDVSSASAADMLIQSTVESLGGLDILITNAGGPPAGSFE
                     VFSEETWQKAVDSSFMSHVRLIRAALPHLRKSSSPSVLTISSFTVKQPLPNLVLSNSI
                     RAATVGLTKSLALELGKENIRFNSIMPGWTMTERVQELMEFRATNNKTTVEEEIAKQT
                     AEIPLGRVGQPQEFANVAVFLVSPAASFVHGVTLAVDGGIIQSPF"
     gene            1090..>2368
                     /locus_tag="VFQ23_18320"
     CDS             1090..>2368
                     /locus_tag="VFQ23_18320"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HET9908610.1"
                     /translation="MNKLSFLARTSLIIAFFFFIDKVLAFVRVGIISRQYAGSVELLD
                     TFNAANNLPDVLFALISGGALAMAFIPLMTEYLTKKGRAAAWDLFSRVANLAFLVTGV
                     AAIIIAIFAEQIVNAEVGIAPGFGADQRKLLAELMRLNLIGTIIFSISGLVMASLQAN
                     QHFILPALAPTMYNVGQIFGALFLVPRFGIYGLVYGVILGAALHLLIQLPALFRYEFK
                     WTPALDLRNSGLIEALKLLGPRLLTMFGIQMIVIARDNLASRLDQVGAVTSLTYGWMI
                     MQVPETLLGTAIATAMLPTLAKFASGEDWSEFRATIEKALRVLVALTIPVAAVMAAGI
                     HPLIRAAFGFDEATSTLITWTTRAYLLTLTGFSIQEIAARSFYARKEPLFPLYAVILR
                     LTLFIAIGIAGLTFFRDIGAPVIAFAEIALLIES"
BASE COUNT          528 a          598 c          613 g          629 t
ORIGIN      
        1 ttactggaag gcatggcttg tggatgtttt ccggttgcag gtgatttaga atccattcgc
       61 gagtggatca cgcccaatgc aaatggcctg ttatttgatt cgaatgatcc aggctcgatt
      121 gcaactgcga ttgtagatgc catccggaat gaagaattgc gaaataaagc tgtagagttg
      181 aatcagaaaa tcattacaac acgcgcggaa taccaacaaa atatgaaaca cgcagaagaa
      241 ttttacaaaa tagtctgtaa tctttgataa ggagaacaca tggacctagg gctcaaaaac
      301 aaacgcgcct tcctcgctgg ctcttcacgc gggcttgggt ttgcgaccgc gctgacgctt
      361 gcaagcgaag gctgtgaggt tgccatcaac agccgggatg aagagaaggt taaagcggcg
      421 gccgaggaaa tatccagcaa aacaggcaca cgggctttcg gcatgacagg agatgtaagc
      481 agtgcctccg cagccgacat gttgatccag tcgacagttg aatcgctggg cggtttggat
      541 atcctgatca cgaacgcggg tggaccaccg gcaggttcat ttgaggtttt ttctgaagag
      601 acatggcaaa aagctgtcga ttcatctttc atgagccacg tgcggctgat ccgtgctgca
      661 cttcctcact tgcggaagtc gtcctctccc agcgtactga ctatttcttc ctttacggtc
      721 aagcagcctc tgcccaacct tgtgctttcc aacagcattc gtgcggcgac ggttggtctc
      781 accaagtctc tggccctgga acttgggaag gagaatatcc gttttaactc catcatgccc
      841 ggctggacca tgaccgaacg cgtgcaggaa ctgatggaat ttcgagccac aaataataag
      901 acaacggttg aagaggagat tgcgaaacaa accgcagaga tcccgcttgg acgtgtaggg
      961 cagccgcagg agtttgcaaa cgtggctgtg tttctcgtct cgcctgccgc atcatttgta
     1021 cacggcgtaa cattggctgt ggatggtgga attatccaga gccccttttg atacaatttc
     1081 tgatcctcca tgaataaact ctcgtttctt gcgcgtacct ccctgataat tgcgttcttc
     1141 tttttcattg ataaggtcct ggcgtttgtg cgtgtgggta tcatttcgcg tcaatacgca
     1201 ggctcggtgg agttactgga tacgttcaat gctgcgaaca acctgcccga cgttttattt
     1261 gctcttatct caggcggcgc tttggcgatg gcttttattc cgttgatgac cgaatacctc
     1321 acaaaaaagg gacgcgcagc agcatgggac ttattctcac gagttgcaaa tctggcgttc
     1381 cttgttactg gagttgcggc catcattatc gctatttttg ctgagcagat cgtgaacgct
     1441 gaggtcggga tcgccccggg cttcggtgca gaccagcgca aattgcttgc cgagctgatg
     1501 cggctcaatc tgattggcac aatcatcttt tccatcagtg ggctagtgat ggccagcctt
     1561 caggcaaatc agcattttat cttgccggcc ctcgcgccga cgatgtataa cgtgggtcaa
     1621 attttcggcg cgctgttcct ggtgccgcgt ttcggcatct acggactggt gtacggcgtc
     1681 atcctcggtg cagccttaca tttgctgatt cagctcccgg ccttgtttcg gtacgaattc
     1741 aaatggacac ctgcgctgga cttgcgcaat agtggattga ttgaagcatt gaaattactg
     1801 gggccacggt tgctgaccat gtttggcatt caaatgatcg ttattgcacg agacaacctt
     1861 gcatctcgtc tggaccaggt cggggcggtc acttcactga cctatggctg gatgattatg
     1921 caagtgcctg aaacgttgct tggcaccgcc atcgctactg caatgcttcc cacccttgcc
     1981 aagtttgctt ccggtgagga ctggagtgaa ttccgcgcca ccattgagaa agctttaaga
     2041 gtcctggtgg cattgacgat ccccgtcgca gcagtgatgg ctgccgggat tcacccgctt
     2101 atacgcgctg catttggctt cgacgaagcg acatccaccc tcatcacgtg gacaactcgc
     2161 gcgtatctcc tcaccctgac tggcttttca attcaggaaa tcgcggcgcg ctcattttat
     2221 gcccgcaaag aacctttgtt cccactttat gcagtgattt tgcgacttac tcttttcatc
     2281 gcaattggta ttgcagggct tacattcttc cgcgatatcg gtgctcctgt cattgcattc
     2341 gcagagatcg ctttgttgat cgagtcga
//
LOCUS       DASTJP010000623        10269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_405374, whole genome shotgun sequence.
ACCESSION   DASTJP010000623 DASTJP010000000
VERSION     DASTJP010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405374"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFQ23_18325"
     CDS             <1..650
                     /locus_tag="VFQ23_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908611.1"
                     /translation="RAYFENVNELDDSIMLFFDGISLPGYGWVFPTGKRTANIGCGVF
                     FEQSFSQASVLRDLIDKHPLLKRILKNARQVGAIKGYPLRIDFSSSLSGNDQVLVVGE
                     AAGLVNPVSGEGIDYALESAQLAANAILNNWKTGLPSSSIQKTYRKALSKTFQTQFMV
                     TRLMQKLYFREGTWPTLLRNASKRPTLRKAMIEACFGVGNPFGVFSPRTLMDIFL"
     gene            complement(672..1316)
                     /locus_tag="VFQ23_18330"
     CDS             complement(672..1316)
                     /locus_tag="VFQ23_18330"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HET9908612.1"
                     /translation="MRLPKHIGIIPDGNRRWANSNGIPKIKGWEKGIDPGYALFKLIQ
                     KAGIEEVTYYGYTMENARRPIIQRNAIINACIQGVKILSQEDAELLVVGNSDSKMFPE
                     ALRPYADKRVKFGKGGIKVNFLVNYGWEWDLSNLKLSGSQEKGIISQLKSREVSKIDL
                     IIRWGGWSRLSGFLPVQSTYADIFIVNELWPDFKPLHLTNALEWYSKQDITLGG"
     gene            1618..2244
                     /locus_tag="VFQ23_18335"
     CDS             1618..2244
                     /locus_tag="VFQ23_18335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9908613.1"
                     /translation="MPLLLLIRHGENEYVKSSKMAGRIQGIHLNEKGRKQAEALGEAL
                     KDFPITALYSSPLERAMETAQPIAQARKLEIFQDPDLMDTDIGKWQGRSWKVLSLTKA
                     WKVVQNAPSRFRFPEGESFMEAQLRYANSLERIGNKHNKPKDIIAVVFHADPIKLAVS
                     HFLGLPLDHFQRLSCNTGSLTMLHVSEMGANLITLNQRPPFDFVSKKN"
     gene            complement(2264..3493)
                     /locus_tag="VFQ23_18340"
     CDS             complement(2264..3493)
                     /locus_tag="VFQ23_18340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9908614.1"
                     /translation="MNPPTEDDHQIQKRNFRNVQIDGVGVSISSVAQPFLPVFLTRLG
                     ATNFQVGLLTSMPGVTGLILALFVGRFLQTRRNIVPWYSLSRLMVILCYALTGILTLV
                     IAQENVIVSTLLIWAFASIPQTALAVAFSVVMNAVAGPEGRYALLSRRWAIFGVTGVV
                     GTFIVTRLIDLISFPLNYAIMFLVLSLGGFLSYYFSRRIELPDQTPPPISDSRSPIEG
                     LSNYISLLRANPAFVSFSLKRFVYFSAMVLSLPIMPLYLVREVQANDAQIGMVTMAMN
                     LVMLAGYYLWPRVSRSRSGRFVLLATTFGMTLYPALSAATSRVELIILYAGIAGLFQA
                     GLDLVFFDELMKTVPPEYSATFVSLAQSMQYLATILAPLAGTWIANTIGLGGALWVSA
                     GLRLLGFLLFLKRDVKR"
     gene            3535..4026
                     /locus_tag="VFQ23_18345"
     CDS             3535..4026
                     /locus_tag="VFQ23_18345"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908615.1"
                     /translation="MFNIRPIKPEETMTAKRVIYRVAHEIFNDSLPLEESILFHESNG
                     ELKDMNNIEQNYFDQDGIFLVTLHEQEIIGTGAIRKLEADICELKRLWLLTDYHGKGL
                     GYRMIGELISVARERGYKRIRLETDPNAQKRAVEFYKRLGFYEIPIPNATPDEDILME
                     MQL"
     gene            complement(4021..4695)
                     /locus_tag="VFQ23_18350"
     CDS             complement(4021..4695)
                     /locus_tag="VFQ23_18350"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET9908616.1"
                     /translation="MKVVIIISAIAEWIAVKPLFPNAKISHFPFGEYLEVTIKEQDVT
                     FFHCGWGKIASAGAIQYLIDHASPDVIINLGTCGGFEGSVQQGEIILVEKTYVYDIME
                     LMGDLDITSYYASSLDLNWLAEPYPHPVRRGMIASADSDLPPDKIAFLKGKGAIAADW
                     ESAALAWVARKNNVRLLILRGVSDMVSEEGGEAYDNIEVFNERAKGIMETLVDQLPDW
                     MNAIRL"
     gene            4754..6595
                     /locus_tag="VFQ23_18355"
     CDS             4754..6595
                     /locus_tag="VFQ23_18355"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9908617.1"
                     /translation="MPEWLGKTIGRVRIEKLLARGGMAEVYLGSHLTLERPVAIKLLH
                     SYIEEDPLLLERFHREAKVVAGLRHSNIVQIYDFDTIDGHPYIVMEYLKGPTLATYLR
                     HLHQRKKRLPSHQVARLLKGLTAALDYAHGQGVIHRDIKPGNILLHNDTDEIPLDQPL
                     TNDVEAVLTDFGLVRVMNASSQTASGFISGTPAYMSPEQARGDQIDHRTDIYSLGVVL
                     YEMLAGRVPFEADSTLTVLHMQIHTSPAPIPGIAPEVQAVMDRALLKNAKERYQSSNE
                     MAVEYYLSIGMNKQAETILKPFPAVKAEPEPDLAEPPVPEPMIQPAPEPVSKPVSTPK
                     TAQAVKAETEPAPKSQRNQSGLRIVLFSFIGLAIVLAVAGYLIFGRGSNTPAATATVS
                     TDVPVANSPVPTEPVVAALPDSTRMVQIPAGNYVVGKDPANNNQSAVQTLALTEFWID
                     QYQVTNQQFQQFAPEWSVSAGEENFPAIGVTWDQADAYCKSLNKRLPNESEWEAAGRG
                     RDANPAVYPWGTDPSDGGNIFDLPNRTYEVGTKDFNVSPFQVYDMLYNVYEWVGEPYG
                     SLEAGNKILRGARFGTPYDLAFRLEVTPNDTNYGQYAGFRCAADQVR"
     gene            6608..8230
                     /locus_tag="VFQ23_18360"
     CDS             6608..8230
                     /locus_tag="VFQ23_18360"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9908618.1"
                     /translation="MKSQIIYRSRWMSYLTILLLLVITGCAGQGVAPTSPPAEPTSTA
                     EPSSPTKDPSSTTPSVEPTVDSKPTADSNVLFQDTFTNPGTGWASAEFDNYFIGYHEP
                     EYYHVEIKSPHLRAPVVGIPNAETNNFPDATIELQVYTVSGRTATDGDYRYGLVFRRT
                     GDNYYAFTISPITKTWEVFKSSPSGIVNLTQGTDEGIQGLDVADLLRVDAQGSNFFFS
                     INNQPVGELTDVDYAAGTVGLYAENIENTNTHVHFDALTIKNVEAPPQEDVGALLYED
                     AFTNPGTGWAAAEFDNYFIGYHEPEYYHVEIKSPFLKAPVVSIPNSETNNFPDATIEL
                     QVYTVSGRTATDGDYRYGLAFRRSGDNFYAFTISPTTKTWEVFKSSPSGIVTLSQGTD
                     EGIQGLDVDDLLRVDAQGSSFLFSINNQLVGQITDTDYPEGSVGLYAENLENTNTHVH
                     FDGLTIRDVTFALSCSILEGGTVNVRNGPGTTFAQIGILSSGDSVKAFGKSANQWIQI
                     ELEGSAEPGWVSYSEGYMSCTPSVDMFPVVSP"
     gene            8402..>10269
                     /locus_tag="VFQ23_18365"
     CDS             8402..>10269
                     /locus_tag="VFQ23_18365"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9908619.1"
                     /translation="MPEWTGKIVGNVRIEKELASGGMGEVYIGTHLRLDRLVTVKVMH
                     SYIEGDPEAQARFEREAKVIAGLRHPNIVQIFDFDIADGHPYIVMEYLRGPSLAAYLR
                     ELYARNEKVQPVQVARLLSIMAAALDYAHEQGVIHRDIKPGNVILHNKTGLFSSDLPL
                     STITEPVITDFGLVRIHTTTQTSAGKRSGTPAYMSPEQARGLTVDHRSDIYSLGMVLY
                     ELIAGHIPFEAETNWGILYKQINEAPPPIPEIPQTVQSVIDRVLAKDPDARYQTCREL
                     AADYLDAIEMFAEAGAVRAGSALPVTRRRELEVSDQTVQRAGPTWKRVVAYSLTGLLF
                     IALAVWGFMTYLSPGANAHTEESVGRLRFQDGTARADQVTISASSLAPPPQGSQYEAW
                     LIQDDGEQRVSIGAIAFDQQNQGLLTYVDSEGQNLIGKYKALEITRETNPDPSPNSSN
                     DIVASVSLPPDGLTHLRHLLFSFNATPEKVGFVHGLNTDTLLVNESAQLMLASFEAGD
                     EAGLGLHAERMLNVILGSQSEEYKDWNGDGTIDDPSDGYGLLLNGENVGYIQGTFTHA
                     NLSLTSPDASPNMILHGGHVKDCATNLDVWTPQLRDQLMAILNASFDSPELEGMVR"
BASE COUNT         2736 a         2544 c         2475 g         2514 t
ORIGIN      
        1 cgcgagccta ctttgagaac gtcaatgagt tggatgactc tataatgttg ttctttgatg
       61 gaattagcct tccgggttat ggctgggtct tccccactgg caaacgaacg gcaaatattg
      121 gttgtggtgt tttctttgag cagtcctttt cgcaagcaag tgttctgcgt gatctaattg
      181 ataagcatcc gttactgaag cgaattctaa aaaacgcgcg tcaagttgga gctattaaag
      241 gttacccact ccggatagac ttttcatctt ctttaagtgg caatgaccag gtattggtcg
      301 ttggagaggc agccgggtta gtcaatccgg tttcaggaga ggggattgat tatgccctgg
      361 aatcagcaca actcgccgcg aatgcaatcc tcaataattg gaaaactgga ctcccatcca
      421 gctcaattca aaaaacatat cgcaaagccc tgagcaaaac atttcagaca caatttatgg
      481 tcacccgctt aatgcaaaaa ttgtatttta gagaaggtac ctggccaact ctgctacgga
      541 acgccagcaa acgaccgact ttgcggaaag cgatgatcga agcctgtttt ggcgtaggca
      601 atccgttcgg tgtattttcg ccgcgcaccc tgatggatat atttctatag cgaagtatct
      661 gtcagccaaa gctaaccacc cagggttata tcctgcttcg agtaccattc cagggcgttg
      721 gttaaatgga ggggcttgaa gtcaggccaa agttcgttca cgataaaaat atcggcatag
      781 gttgattgaa ctggtagaaa tccactgagt ctgctccagc cgccccagcg aatgatcaag
      841 tcgatctttg agacttcgcg cgatttcagt tggctaatga tgcctttctc ctgacttccg
      901 gatagtttca ggttgctcag gtcccattcc catccgtaat tgaccagaaa gttaactttg
      961 atgccacctt ttccaaattt gaccctcttg tccgcataag ggcgaagagc ttcaggaaac
     1021 attttggaat cgctgtttcc aacgaccagc aattctgcgt cttcctgaga gagaattttt
     1081 acgccctgga tacaggcatt gatgatcgca tttctctgga taattggtct tcgggcattt
     1141 tccatggtgt aaccgtaata agtcacctcc tcgatcccag ccttctggat caatttaaag
     1201 agagcatacc cgggatcgat tcccttttcc caccccttaa ttttaggtat accgttggaa
     1261 ttagcccacc ttctatttcc atctggaata atgccaatat gttttggtaa tcgcatggaa
     1321 aacctcggcg tttcgtaaat tttgaattgt cgtaaaagct gatttgttgt tctgcagttc
     1381 taccacgttg aacttttaac gtcccgatta tataatcaca tgttacaaaa agcaatttca
     1441 acgtcaatct tgtccggttt gatgaacggc tgttcattct gtaaatacgc taattattga
     1501 agcttgaatc cttactgttt caatcgatta tgcgaatttt ttgttatgaa cccgtgtttt
     1561 ctctccgact caaggctgat agaattagag aatctcctta ttagattgga tacatccatg
     1621 cctcttctgc ttctcatcag acacggcgaa aacgaatacg taaaatcatc caaaatggca
     1681 ggtcggatac aggggattca tctcaatgaa aaagggcgga aacaggctga agcattaggc
     1741 gaagctctaa aggattttcc tattactgct ctctactcca gccctcttga gcgggcaatg
     1801 gaaacggcac agccgatcgc gcaagcgcgc aagctggaaa tctttcaaga tcctgatctc
     1861 atggatacgg atatcggtaa atggcaaggc aggtcctgga aagtcttaag ccttacaaaa
     1921 gcatggaaag ttgtgcaaaa tgctccttct cgctttcggt ttccggaagg cgaatcattt
     1981 atggaggcgc agttgcgcta tgcaaactca ttagaacgca tcggaaataa acataacaag
     2041 ccgaaggata tcatcgcggt ggtctttcac gccgacccga tcaaactggc ggtttcgcac
     2101 tttctgggat tgccgctcga tcacttccaa cgcctgagtt gcaatacggg gtcactgacg
     2161 atgctgcatg tcagtgaaat gggtgcaaat ttgatcacgc tgaatcaacg cccaccgttt
     2221 gactttgtct ccaaaaaaaa ttgaagcacc gatgcaactt gcgttatcgt tttacgtcgc
     2281 gctttaggaa taacaggaac cccagtaatc tcagcccagc actcacccag agcgctcctc
     2341 ccaaaccaat agtatttgca atccacgtcc ctgctaatgg cgcgaggatc gtcgccaggt
     2401 attgcatgga ttgcgccaga gagacaaagg tcgcgctata ttcaggcggt acagttttca
     2461 tcagttcatc gaagaagacg agatccaacc cggcttgaaa cagcccggca atgcctgcgt
     2521 agaggatgat caattcgacg cgcgaggtcg ccgcgcttaa tgcggggtag agtgtcatac
     2581 caaaagtcgt cgccagcaaa acaaatcgtc cactacgtga tcgtgagact cggggccaaa
     2641 gatagtagcc ggcaagcatg acaagattca tcgccatagt gaccatacca atttgcgcgt
     2701 cgttggcctg gacttcacga acgagataca aaggcatgat cggcaggctt aataccatgg
     2761 ctgaaaaata aacaaagcgt ttcagtgaaa aagatacgaa ggctgggttc gctcgcaaaa
     2821 gactaatgta attacttagt ccttcgattg gcgagcggct atctgaaatc ggcggagggg
     2881 tttggtcagg taactcaatc cgtctggaaa aataatagct caaaaaacca cccaacgata
     2941 acaccaggaa catgatcgca taattgagcg gaaatgagat caagtcaatc aggcgtgtga
     3001 cgatgaaagt accaactaca cctgtcacac caaaaatcgc ccagcgtcgg cttaataagg
     3061 catacctacc ctccggtccc gccaccgcgt tcattacaac ggaaaaagcc accgccaggg
     3121 cagtttgagg aatcgaagca aacgcccaga tcaacagtgt ggaaacaatc acattttcct
     3181 gggcaatcac cagagtcaat atccccgtca acgcgtaaca taagatcacc atcagccgag
     3241 agaggctata ccacggcacg atattgcggc gcgtttgcag gaagcgccca acaaaaaggg
     3301 ccagaatcaa tcccgtgacg cctggcatgg aggtgagcag acccacctga aaattagttg
     3361 ccccaaggcg cgttaggaat acgggcagga aaggttgagc aacgctggag atgcttacgc
     3421 caacaccatc gatctgaacg tttctaaaat ttcttttctg gatttgatgg tcgtcttcag
     3481 tcgggggatt catggacaca acggattata acgctggcag agtacaatta aagaatgttc
     3541 aacattcgcc ctattaaacc agaggaaacg atgactgcaa agcgagtcat ttatcgcgtt
     3601 gctcatgaaa tcttcaatga ttcgcttccg cttgaggaat cgattttgtt tcatgagtcc
     3661 aatggcgagc tcaaggacat gaataatatt gaacaaaact attttgatca agacggaatt
     3721 tttctcgtca ccttgcatga acaggagatc attggcaccg gagccatacg aaaattagaa
     3781 gctgacatct gtgaactcaa acgactctgg ctgcttacgg attatcatgg caaagggctg
     3841 ggctatcgga tgattggtga attaatttct gttgcaagag aaaggggata taaacgcatc
     3901 cgacttgaaa ccgaccctaa cgctcagaaa cgagccgtcg agttttacaa gcggctcggg
     3961 ttctatgaaa ttccgattcc aaacgccact cccgatgaag acatcctgat ggaaatgcag
     4021 ctataacctt attgcgttca tccaatcggg cagttggtct accaatgtct ccataatccc
     4081 tttggcacgt tcgttgaaca cttcgatatt gtcgtaggcc tctccccctt cttcgctcac
     4141 catgtcactc acgccgcgca agatcaataa tctcacattg ttctttcggg ccacccaggc
     4201 aagtgcagca gactcccaat ccgcggcgat ggcgcctttg cctttgagga aagcaatctt
     4261 gtccggcgga agatcgctat ccgcggaggc gatcatccca cggcggaccg gatgaggata
     4321 aggttcagcg agccagttca agtccagtga tgatgcgtaa tagcttgtga tatccaggtc
     4381 ccccataagt tccataatat cgtatacata agtcttttcg actaaaataa tttctccttg
     4441 ctggactgag ccttcaaagc caccacacgt cccaaggtta atgatcacat caggcgaggc
     4501 atgatcgatc aaatactgga tcgctcctgc ggaggcgatt ttcccccagc cgcaatggaa
     4561 gaaagtaaca tcttgctcct tgatcgtcac ttctaaatat tcaccaaatg gaaagtgact
     4621 gatctttgca ttcgggaata aaggcttgac ggcgatccat tcagcaatcg ccgagatgat
     4681 gataactact ttcattcttt ttccatttta gattagattt cttcgcataa ttgtcgtata
     4741 attaatttta catatgcctg aatggcttgg aaaaaccatc ggacgagtcc gaattgaaaa
     4801 actccttgca cgggggggaa tggcagaagt ttatttgggt tcgcatctga ctcttgaacg
     4861 cccagttgcg atcaaattac tgcacagcta tatcgaagaa gaccctcttc tcctcgagcg
     4921 atttcaccgc gaggcaaaag tagtagcggg attacgacat tccaatatcg ttcagatcta
     4981 cgatttcgac acaatcgatg gacaccctta tattgtaatg gaatatttga aggggcccac
     5041 gctcgcaacc tatcttcgtc acctgcacca acgcaagaag cgccttccct cccatcaagt
     5101 ggcgcgcctt ctcaaggggc tgacggcggc cctcgattac gctcacgggc aaggcgtcat
     5161 tcatcgggac atcaaaccag ggaatatcct gctgcacaac gataccgatg aaattccgtt
     5221 ggatcaacct ctgacgaacg atgtagaggc agtgctcacg gacttcgggc tagtgcgcgt
     5281 gatgaacgca tcttcacaaa ctgcatccgg ctttatcagt gggacgccag cctacatgtc
     5341 tcctgagcaa gcgcgcggcg accagatcga tcaccgaacc gatatttatt ctctgggtgt
     5401 cgtgctgtat gaaatgctgg ccggccgcgt cccatttgaa gcagatagta cgctgacggt
     5461 tctccacatg cagattcaca catcgcccgc accgatcccc ggcatcgctc cggaagtgca
     5521 ggcggtgatg gaccgggccc tgctgaaaaa cgcgaaagaa cgctaccaaa gcagcaacga
     5581 gatggctgtt gagtattacc tttccattgg gatgaacaag caagcggaaa cgatcctaaa
     5641 gccatttccc gcggtgaaag cggagccgga acctgatctg gcagagccac ccgttccgga
     5701 gcccatgata cagccggcac cagaaccggt ctccaagcca gtctccaccc caaaaacagc
     5761 gcaagcagtc aaggctgaga ccgagccagc accaaagtca cagcgaaacc aatcgggcct
     5821 gcgtattgtg ttgttttcat ttatcggtct tgcaatcgtc ctggcggtcg cgggatattt
     5881 gattttcggt cgcggctcga atacacccgc cgcaacggcc acggtaagca cagatgtacc
     5941 ggttgctaat tcacccgtcc ccaccgaacc cgttgtggca gccttacccg actctaccag
     6001 gatggtacaa atccccgccg gcaattatgt agttggaaaa gatcccgcca ataacaacca
     6061 aagcgcagtc caaacccttg cgttaactga gttttggatc gatcaatacc aggtcaccaa
     6121 tcagcaattt caacagtttg cccccgaatg gtcagtctcc gctggagaag aaaatttccc
     6181 cgctataggc gttacgtggg atcaagcaga tgcttattgt aaatcactga ataagcgtct
     6241 gcccaacgag tcggaatggg aggcagctgg gcgcggacgg gatgcaaatc ccgccgtata
     6301 tccgtgggga acggatccct cggatggcgg gaatatattc gatctgccaa atcgtaccta
     6361 tgaagtcggt accaaagatt ttaatgtcag cccattccag gtctatgata tgttatacaa
     6421 tgtgtatgaa tgggttggcg agccatatgg cagccttgag gcagggaata agattttgcg
     6481 cggcgcgcgt tttggcaccc cgtatgattt agcttttcgg ctggaagtta cacccaatga
     6541 tacaaattat ggccaatacg ctggcttccg ttgtgcagcc gatcaggtaa ggtaaaggag
     6601 aagtcgtatg aaaagccaaa tcatttaccg atccaggtgg atgagctatt taacaatctt
     6661 gttgctcctg gtgataacgg gatgtgcagg tcagggggtt gcgccaactt cgccaccagc
     6721 agagcccacg tcaaccgctg agccatcctc accaacgaag gatccttcgt ccacaacacc
     6781 cagcgttgag ccgactgtag attcgaaacc taccgcagat agcaatgtgt tgtttcaaga
     6841 tacattcacg aatcctggca ctggttgggc atccgctgaa tttgataatt atttcattgg
     6901 ctatcatgaa ccggaatatt accatgtgga gatcaagagt cctcacctga gagcaccagt
     6961 cgtgggcatt cccaatgccg aaacgaacaa ttttcccgat gcaaccattg aacttcaggt
     7021 ctatacagta tccgggcgaa ccgcaacgga tggtgattat cggtatggtc tggtgttccg
     7081 tcgtactggc gataattact acgcttttac catctctccg atcaccaaga cctgggaagt
     7141 attcaagagt tcacccagcg ggattgtgaa cctcacccaa ggcacagatg agggcataca
     7201 gggattggat gttgctgatc tgctgcgagt ggatgctcag ggatctaatt tctttttcag
     7261 catcaataat caacccgtcg gcgagttaac agatgtagat tatgcagccg gaaccgtcgg
     7321 cctctacgca gaaaatatcg aaaacaccaa cacccatgtc cactttgatg cattaacaat
     7381 caaaaacgta gaggcacctc cccaggagga tgtgggggct ttgctgtatg aagatgcatt
     7441 tacgaatcct ggcaccggat gggcagcagc tgaatttgat aattatttca ttggctatca
     7501 cgaaccagaa tattatcatg tggaaatcaa aagtccgttc ttaaaggctc ctgtcgtgag
     7561 cattcccaat tccgaaacga ataatttccc cgatgcaacc attgaacttc aggtctatac
     7621 agtttccggg cgaaccgcga cggatggtga ttatcgttac ggtctggcgt tccgtcgctc
     7681 tggtgacaac ttttacgcct tcacgatctc tccaaccacc aagacctggg aagtgttcaa
     7741 gagctcaccc agcgggattg tgaccctctc gcaaggcaca gatgagggca tacaggggtt
     7801 ggatgttgac gatcttctac gtgttgatgc ccagggatcc agtttccttt tcagcatcaa
     7861 taatcagctg gttggtcaga tcaccgacac ggattatccg gaaggaagcg tcggtcttta
     7921 tgcagaaaat ttggaaaata ccaataccca tgtccatttt gatgggttga ccattcggga
     7981 tgtgaccttt gctttaagtt gcagtattct cgaaggtgga accgtgaacg tacggaatgg
     8041 accgggtacc acgttcgctc aaattggtat tttatcgagt ggtgacagcg tcaaagcctt
     8101 tggaaaaagc gccaatcaat ggatccagat cgaattggaa gggagcgcag agccaggttg
     8161 ggtttcctac tctgaagggt atatgtcctg cacaccatct gtggatatgt ttccggttgt
     8221 cagtccataa ctagaaatag actcacgcac gatctgttgt tgaaaatcaa taaggttttg
     8281 catcccgaca cctcttatcg aagaacgata agaggtgttt taatttcgct aatgtatagg
     8341 ctgagataaa tcatggtgtt tcgcgtcaca gccctctccc tgcttgctgt ataattccgt
     8401 tatgccagag tggaccggaa agattgttgg aaacgttcgc attgaaaaag aactcgccag
     8461 cgggggtatg ggagaggtct atattggcac tcatcttcgg ctcgatcgtc ttgtcactgt
     8521 taaagtgatg catagctata tcgaggggga tcccgaggcg caagctcgct tcgagcgcga
     8581 ggccaaggta attgcgggac tgcgtcaccc caacatcgtg cagatctttg acttcgacat
     8641 cgccgacgga cacccataca ttgtgatgga gtatctaaga ggaccatcgc tggccgccta
     8701 tttgcgcgag ctgtacgcgc gcaatgaaaa agttcaaccc gttcaagttg cacgactctt
     8761 aagcatcatg gcagcagctc tcgattatgc acatgagcag ggagtcattc atcgcgatat
     8821 caagccaggc aatgtaattc tgcataataa aacgggattg ttttcaagcg acttaccact
     8881 tagcacgatt accgagcctg tcattacgga ttttggattg gtccgtattc atacgacaac
     8941 gcaaacatcg gccgggaaaa gaagcgggac cccagcttac atgtcaccgg agcaggcacg
     9001 tggtttaaca gttgatcatc gtagtgatat ctattccctt ggtatggttc tttatgaact
     9061 gattgccggg catatcccct tcgaagcgga gacaaactgg gggatcctgt acaagcagat
     9121 caacgaagca ccgccaccga ttcctgaaat cccgcaaacc gttcaaagtg taattgaccg
     9181 tgtcctggca aaagatcccg atgctcgcta ccagacctgt cgtgaacttg cagccgacta
     9241 cctggacgcg atcgagatgt ttgcggaggc aggtgctgtg cgggcgggat cagccctgcc
     9301 ggtcacccgt cggcgcgagt tggaagtatc cgaccaaacc gtgcaacgcg caggaccaac
     9361 gtggaaacga gtagttgcat attccttaac cgggcttctt ttcattgctc tagcagtttg
     9421 gggattcatg acatatttgt ctccgggtgc aaacgctcac acggaagaat cagtcggtcg
     9481 attgcggttt caagatggga ctgcccgcgc ggaccaagtg accatctccg cctccagcct
     9541 ggctccccca ccacaaggca gtcagtatga ggcctggctg attcaggatg atggagaaca
     9601 gcgcgtcagc atcggtgcga ttgcgtttga ccagcaaaac cagggattac tgacttacgt
     9661 cgatagcgaa ggacaaaatt tgatcggaaa atacaaagcc ctggaaatta ctagggaaac
     9721 caatccggac cccagcccga attcttcaaa cgacattgtt gcttcggtaa gcctcccacc
     9781 ggatggtctg actcatttaa ggcatctctt gttttcgttt aatgctacac ccgagaaagt
     9841 gggattcgtt cacggcttga atacggatac actcctggtc aacgaatccg cgcagctaat
     9901 gcttgcgtcc tttgaagccg gggatgaggc aggtctcggt ctgcacgcgg aaagaatgtt
     9961 aaacgtgatc ctcggaagcc agagtgagga atataaagat tggaacggcg acggaaccat
    10021 tgacgatccg agcgatggat acggactctt actcaacggg gaaaacgtgg gatatattca
    10081 gggtactttt acccatgcaa atttgtcact cacatcacca gacgcatcgc cgaatatgat
    10141 cctccatggc gggcacgtaa aggactgtgc caccaatctc gacgtgtgga cccctcaatt
    10201 gcgcgatcaa ttgatggcta tcctgaacgc atccttcgat tcgccagagt tggagggtat
    10261 ggtgcgtga
//
LOCUS       DASTJP010000624         5584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_520547, whole genome shotgun sequence.
ACCESSION   DASTJP010000624 DASTJP010000000
VERSION     DASTJP010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520547"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1273
                     /locus_tag="VFQ23_18370"
     CDS             116..1273
                     /locus_tag="VFQ23_18370"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9908620.1"
                     /translation="MQKTLRSVTNLFLVIIFFSACSPQAPASTPDMSAALTRAVETAL
                     AQLQPTLTPLPSETPIPPPTAVRTPPALPDKFTASQLSPLDAPHTYIDDTCQYLYDKW
                     NSNNAPPGTVVMVVMFHGIVKDAVAQDAEDITARDFNQLMNDLKEQGFEAINMTQMAD
                     FIDHNARIPPRSVLLISDDRHQGAYFNDHFRPYRDQWGWPVVNGWISFEDGPRALSLE
                     DNIALEAEGWVDHQSHGYIHNINMSDASDDEFIRTEFERSIADLQTNFRKTPVAIIWP
                     GGNFGSRPVQFAREYGLRLGFTINPRGPIMYNWVPLADQPDPARPAFLPEGQVNDPRM
                     VLPRYWPYQVQSNLDSVRTIGNDAAAYAEQNKAVELEYYDIMCAPTLGQIP"
     gene            1340..3598
                     /locus_tag="VFQ23_18375"
     CDS             1340..3598
                     /locus_tag="VFQ23_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908621.1"
                     /translation="MDLQSYIPGLVPAEPGPLSRFIPPLEEGVVSAWLSLNVPASTWL
                     LDPFGFSPRLVLEAARAGYRVLVTGNNPITRFLLKMTADPLQESDFTAAMADLSAAKK
                     GEERLSAHLQSLYLTRCEECSLEMEADAFLWRKGEDAPYARIYTCPRCEDSGERPATK
                     EDIDRARKIASTDNLHRSRAFERVVQLEDEDRVFAEEAIEHYLPRPLYVLTTIINRLD
                     SLNLTSERRRALNAILLVAFDSGNTLWSHPAERRRPKQLNIPTQFREHNLWMQLERGL
                     SLWTETASGVPCKAWPERIPESGGICIYEGRLKDLAQEVKKVIPISAVIGSLPRPNQA
                     FWTLSALWSGWLWGREAVEPYKLVLRRRRYDWAWNATALNSAFAHLFELLPVGTRFFG
                     LLSEPEASFLTSALTAASAAGFELQSAALRTEHDPIQLLWRRGEHVKRTMNEASIKTV
                     SDAIYSHLKERGEPASYFHVHAAGMLALTEAHMLKGKDQEFDDALRSTHTLIQRALSE
                     DKRFVHYSTGESVESGFWGSSTVTEQGSEVNRYYESLADRVEIAIVTFLQKNPNSIYL
                     DLEGDLYPRFPGLLTPSKGMIYAVLNSYAEKNGAAWKLRGEDLASARRNELTIITAMI
                     EAVGKRLGYVTRKQEKNYLWEETSTGELTPNLHYAFYILASALIGRAISETPYPPEKT
                     IIVIPGGRAALAEYKKQRDPALAATMQRYQLVKYRLLRAMVEVPVLTRETFEEQIASD
                     PLEKSKSQMMMF"
     gene            3614..4741
                     /locus_tag="VFQ23_18380"
     CDS             3614..4741
                     /locus_tag="VFQ23_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9908622.1"
                     /translation="MNTKLFVTAFMITILLVACTGQTPAPTDQPTSNETSPPTSTQAP
                     VATRTVGTPLLTNTALAETSAVLPTATLTPALPQPTNAADCTNLAAFVTDVTIPDNSN
                     VTGGTLFTKTWRIRNTGTCTWGSGYTLSHYSDEEMGAPEAIPLNVTQPGQEIDISVDL
                     RAANAVGTHRGNFVIKNPAGLIMQIDNDSRLWVIINVTDTITATPSSTPDTTGSSATG
                     TSIVIAIDNTSGFANATCSSATDSGKLAETFNAINAYRAQNNLGEYTLNIKLAQAAQA
                     HANDMACNKIFVHTGSDKSTPQTRVRISGYEASSVTENVYGSFPPLTGQGAVNWWIND
                     KTDLRHNLNLLSTIYSEIGVGYSFFDNFGFYVIVFAAPSVR"
     gene            4874..>5584
                     /locus_tag="VFQ23_18385"
     CDS             4874..>5584
                     /locus_tag="VFQ23_18385"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9908623.1"
                     /translation="MKKIWTLFLIASISLGSCVTVSEETPAPVAPVFVTSTLRPTRPA
                     LSLPTDIPPTSTPDPSTTTTPDSPDGTSTATTVCTDGAVLLEDVTVPDNALMTPGEKF
                     TKTWRLMNTGKCNWSGYTIAFFAGDRMASPDSAPVPQTDAGKTVDVSVELTAPSVDGA
                     YTGFYELRNAENETLLIGTESTFWVKILIGNATASNSSPVPTTSSGTPGSRPSGPASC
                     SYTSSSSYLNEITTLINNA"
BASE COUNT         1377 a         1532 c         1402 g         1273 t
ORIGIN      
        1 ttattaacgg cgattccacc gagtaaattt cggggtccaa gactcccgac gggtttcaat
       61 aacagtgaga cggcaggtga cctgccgtct cattcttttc ggataaaatc atctcatgca
      121 aaagactctt cgaagcgtta caaacttgtt cctagtgatc attttcttta gtgcttgttc
      181 cccacaggca cctgcctcca cacccgatat gagcgcggca ctgacgcgag ctgttgaaac
      241 cgcgttggca cagttacagc ccactctaac tccattacca agcgaaacac ccattccacc
      301 tccgacggcc gtccgaacac cacctgcgct tccggataaa ttcaccgcca gccagctgag
      361 tccgcttgat gcgccgcata cctatattga tgacacctgt cagtatctgt atgataaatg
      421 gaattccaac aacgccccac ccgggactgt cgtgatggtg gtgatgtttc atggaattgt
      481 caaagatgcg gtagcgcaag atgcggaaga tatcacagcc cgagatttca accaattgat
      541 gaacgatctg aaagagcagg gctttgaagc cattaacatg acccagatgg cagatttcat
      601 cgatcacaac gcaaggattc cgccgcgttc cgttctgctc atttcagacg accggcatca
      661 gggcgcgtat ttcaacgatc atttccgtcc ctatcgtgat caatggggct ggccggtcgt
      721 caatggctgg atcagcttcg aagacgggcc tcgcgcgctc agcctggagg ataacattgc
      781 tctcgaggca gaagggtggg tggatcacca atcccacggg tacatccata atatcaacat
      841 gagcgatgcc tctgacgacg agtttatcag gactgagttc gaaagatcca tcgccgatct
      901 acaaacaaac ttccgcaaaa ctccggtggc cattatctgg cccggcggca attttggaag
      961 ccgccctgtg caatttgccc gtgagtatgg gttgcggctt ggcttcacga tcaatccgcg
     1021 cggtcccatt atgtacaact gggttccgct ggctgatcaa ccggatcccg cacgacctgc
     1081 ttttctgccc gaaggtcagg tcaatgatcc gcgcatggtg ttgcctcgct actggcccta
     1141 tcaggtgcag tcaaatcttg attccgttcg tacgatcggc aacgatgccg cggcctacgc
     1201 agagcaaaat aaagccgttg aactcgagta ttatgacatt atgtgcgcac ccaccctggg
     1261 ccaaatccca taataattgt ggggcaagtt ggcatcttgc cctgcaaaaa ttttatgggg
     1321 atgtatactt aaacaaacaa tggatctcca atcgtacatt ccggggttag ttcccgcaga
     1381 gccagggccg ctctcccgat tcataccgcc tctggaagag ggagttgtct ctgcctggct
     1441 ttctctgaac gtccctgcct cgacatggct gctggacccg ttcggattct ctccccggct
     1501 ggtgctcgaa gccgcgcgtg ccggataccg cgtcttggta actggcaata accccattac
     1561 tcgctttctc ctgaaaatga ctgcagaccc actgcaagag tcagacttca cagctgcgat
     1621 ggctgatctc tctgctgcaa aaaaagggga agagagactg agcgcgcatc ttcaatccct
     1681 ctatttgaca cgatgcgaag aatgcagctt ggagatggaa gccgacgcct ttctctggcg
     1741 gaaaggcgaa gacgcgccct atgcccgtat ctatacttgc ccccgctgtg aggattcggg
     1801 tgaacgcccg gcaacaaagg aggatatcga tcgcgcacga aagatcgcct cgacagacaa
     1861 cctgcatcgt tcacgggcct ttgagcgcgt tgtccaattg gaggatgaag accgggtctt
     1921 tgcagaggaa gccatagagc actatctgcc ccgccctctc tatgtgttga ccacaattat
     1981 caatcgcctt gatagcctca atctaacgtc agagcgcagg cgggcgttaa atgccatcct
     2041 ccttgtggca ttcgactcgg gaaacacgtt atggagtcat cccgccgaaa gacgccgccc
     2101 aaagcaactg aacattccca ctcaattccg tgaacacaac ctgtggatgc agcttgagcg
     2161 cggattaagt ctatggaccg aaacggcctc tggcgtaccc tgcaaagcat ggcccgagag
     2221 gatcccggaa agtggtggca tttgtatcta cgaaggtcgg ctcaaagatc tcgcgcagga
     2281 ggtcaagaag gtaattccga tttctgcagt catcggttcg ttgccgcgac cgaaccaggc
     2341 gttctggact ctatccgcat tatggtccgg ctggctttgg gggcgtgaag ccgtggaacc
     2401 ttataagctg gtattgcgac ggcggcgcta tgactgggca tggaacgcga ctgcgttgaa
     2461 ttctgcgttt gcgcatttat ttgaactgct tccggttggc actcgattct ttgggttgct
     2521 ttcggaaccc gaagcctcct ttctgacctc tgctctgacc gctgccagtg ctgcaggctt
     2581 tgagttgcag agcgctgcct tgcgaacgga gcatgaccca atccagcttt tgtggcggcg
     2641 cggcgaacat gtgaagcgca caatgaacga agcaagtatc aaaaccgtca gcgacgctat
     2701 ttactcacat ctgaaagaac gtggcgagcc ggccagttac ttccatgtcc atgcagcagg
     2761 catgctggct ttgacagaag cccacatgct taagggcaag gaccaggaat ttgatgacgc
     2821 gctccgctca acacacacac ttattcaaag ggcattatcg gaagataaac gatttgtcca
     2881 ctactcaact ggcgaaagtg tagaaagtgg attttggggt agcagcaccg tcacagagca
     2941 gggctctgag gtcaatcgct actacgaatc gcttgccgac cgcgtggaaa ttgcgattgt
     3001 cactttcctg caaaaaaatc cgaacagtat ctatctggac ctcgaaggtg atctctaccc
     3061 acgtttcccg ggcttgctga caccatccaa ggggatgatc tatgctgtgt tgaattccta
     3121 cgcggaaaaa aacggcgcgg catggaaact gcgaggcgaa gaccttgctt ctgcccgacg
     3181 caatgaactc acgatcatca ccgccatgat cgaggcggtc ggcaaacggc tggggtatgt
     3241 cacgcgcaaa caggaaaaga attatctctg ggaggaaaca agcacgggcg agttgacccc
     3301 gaacttacac tacgcgtttt atattctggc atctgctctg attgggcgtg caatctctga
     3361 gacgccctac ccgccggaga aaacaataat tgtgatcccg ggcgggcgcg cggcacttgc
     3421 agaatacaag aagcagcgtg accctgctct ggcggccacc atgcaacgct atcagcttgt
     3481 aaaatatcgt ttgttgcgcg cgatggtgga ggtcccggtg ctgacccgtg agacgtttga
     3541 agagcagatc gccagcgatc cgctggaaaa gtccaagtca cagatgatga tgttttgaag
     3601 tggagaggca aagatgaata caaaattgtt tgtcacggca ttcatgatca cgatactgct
     3661 tgttgcctgc actgggcaaa caccggcacc gacggaccag ccaacatcca atgagacgtc
     3721 accacccaca tcaacgcagg cccctgttgc cacgcgtacc gtgggcaccc cacttctgac
     3781 caatacagct ctcgcagaaa catcagccgt tctgccgaca gccactctga ctcctgcgct
     3841 gccacaaccc accaatgcgg cagattgcac caatctcgct gcgtttgtca cagatgtgac
     3901 gatcccagat aacagcaatg tgactggagg cacgctcttc accaagacct ggcgtattcg
     3961 caataccggc acttgtacct ggggatctgg ctatacattg tctcactatt cggacgagga
     4021 aatgggcgca ccagaggcaa taccgttaaa tgttactcag ccagggcagg aaattgacat
     4081 ttcagtggat ttgagagcgg cgaacgcggt cggaactcat cgaggcaatt tcgttattaa
     4141 aaacccggca gggttgatca tgcagattga caacgattca cggctttggg tgatcatcaa
     4201 tgtcacagat accatcacgg caacgccttc cagcacgccc gataccactg gcagttcggc
     4261 cactggaacc agcatcgtca tcgcgattga caacacctct gggtttgcga acgccacctg
     4321 ttcgtccgcc acagactccg ggaaactggc tgaaaccttc aacgcgatta atgcctatcg
     4381 agcacaaaat aacctgggag aatatacgct gaacatcaag ctcgcacaag ccgcccaggc
     4441 gcatgccaac gacatggcct gtaacaagat ctttgttcac accggttccg acaaatcaac
     4501 gccacagacc cgtgtgagga tcagtggtta tgaggcttcc tctgtaacgg aaaatgtata
     4561 tggcagcttt cctcctttaa caggtcaggg tgcagttaat tggtggatca acgacaaaac
     4621 cgatttgcgg cataacttga atttgctaag caccatctac tctgaaattg gcgtggggta
     4681 ctcctttttt gataatttcg gcttctatgt gatcgtattt gccgcacctt ctgttagata
     4741 ggctcgtgac ctcggcttgt tgcatgggtt acgaatcacg cttcatttga gggcaaattc
     4801 ccgtcttaaa ttctaagttt aattttagat aatgatgtgg cgtcgggcgt ctatttcaat
     4861 gtacaatttt cgcatgaaga agatttggac actatttttg attgcgagta taagcctggg
     4921 ttcctgtgtc acagtttcag aagagacacc tgctcctgta gcacccgtct ttgtgacgtc
     4981 cacgctgcgg ccgacccgac cagccttatc cctaccgact gatattcctc caacctccac
     5041 gccagacccg tcaacgacaa ccacgccaga cagtcctgat gggacatcta cagctacgac
     5101 agtctgcaca gatggcgccg tcctcctcga ggatgtaaca gttcccgata acgccttgat
     5161 gaccccaggg gagaaattca cgaagacctg gcgattaatg aacaccggga aatgtaattg
     5221 gagcggatac accatcgcgt tcttcgcagg cgaccgcatg gcatcgccag attccgcgcc
     5281 cgtcccacaa acggacgcag gtaaaacagt ggatgtctct gtcgaattga ccgcaccttc
     5341 tgtggatggc gcgtataccg gattttatga gctgaggaat gcagagaatg aaacactctt
     5401 aataggaacg gaaagcacat tctgggtcaa gatattgatc ggcaacgcaa ctgcgtcaaa
     5461 tagtagtccc gtcccaacga catccagtgg cactcctggc tccagaccca gcggacccgc
     5521 tagttgcagc tataccagca gttcatccta tctgaatgag attacaacgc ttatcaacaa
     5581 tgcg
//
LOCUS       DASTJP010000625         5951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_684170, whole genome shotgun sequence.
ACCESSION   DASTJP010000625 DASTJP010000000
VERSION     DASTJP010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684170"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFQ23_18390"
     CDS             <1..467
                     /locus_tag="VFQ23_18390"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HET9908624.1"
                     /translation="AAQASKATGAVIASHTIGGKVAKEEMDVLEAAGHDLQRFIWIHA
                     QSEPDVTVLKEAAARGAYIELDSIGAPNQSEAELVDTILALMEAGYLDHILLSHDAGW
                     YNPGRPDGMPDHGFRGYTALTKEFLPAVSARGISDELIQHITMSNPARAFAF"
     gene            591..752
                     /locus_tag="VFQ23_18395"
     CDS             591..752
                     /locus_tag="VFQ23_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908625.1"
                     /translation="MNTIEMIWRGSVLVVLGYFAWSSFLAGSILAVFILLFCIPVWLF
                     IGLYNIGYF"
     gene            905..1159
                     /locus_tag="VFQ23_18400"
     CDS             905..1159
                     /locus_tag="VFQ23_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908626.1"
                     /translation="MNSLNLSTQSYFYWLPPVTLTDTNSLDKSGTYAACEQMIQMPFM
                     MMGLSNLDEMLERLEKNGRLSAKEHQSLVELARKTWRVNS"
     gene            1307..1738
                     /locus_tag="VFQ23_18405"
     CDS             1307..1738
                     /locus_tag="VFQ23_18405"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9908627.1"
                     /translation="MNIEQLPEPRVEIFSEPSFLEKAANFLESDVVIFDSSTGYERHG
                     VDGYIQYLTEFVQIIPDLTSKVIDHQTYDDKLISRLQMQGKFTGTLRTDEGIFFGNGN
                     PIDIEYQVEQEFNHLGKVRRLVFNYDLPEFLFQLSRRYRAS"
     gene            complement(2552..2788)
                     /locus_tag="VFQ23_18410"
     CDS             complement(2552..2788)
                     /locus_tag="VFQ23_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908628.1"
                     /translation="MSVGGTGELVLVGMVVGVFDANGFELDPRLGRFVLVIGTNVEVA
                     VSVADSGVTEGDAVTVPGNAVLEADGLMVGVIKC"
     gene            complement(2834..3136)
                     /locus_tag="VFQ23_18415"
     CDS             complement(2834..3136)
                     /locus_tag="VFQ23_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908629.1"
                     /translation="MVITFVHAAKALVERRLLPLRPHGLVSVFVGGGSDVSVAGGADV
                     SVGAGADVSVGRGAEVFVGAGADVSVGTGTEVSVGGGGEVSVGDGTGVRVLVGLGV"
     gene            3579..4343
                     /locus_tag="VFQ23_18420"
     CDS             3579..4343
                     /locus_tag="VFQ23_18420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET9908630.1"
                     /translation="MLQFLEENYQGPAFELWGITHLGAIGVIIVLNLLLISSKNASDG
                     TKTSIRWLLALILIANEIAWHYWNVAYDNWTIQTMLPLHLCSLLVWLGALMLVTRSYR
                     IYEFMYFMGIGGAIQALATPGIGIYGFPHFIFFQYFISHGLIITSAIYMTTVIGLRPT
                     WKSILRVVIWMNIYVVIIYFVNVAIGSNYLMLLEKPSTPSLLDRLPEWPLYILYMEGI
                     GLVTCILLYLPFAIKDWRHKYLMNKDNASRLESISK"
     gene            complement(4621..4833)
                     /locus_tag="VFQ23_18425"
     CDS             complement(4621..4833)
                     /locus_tag="VFQ23_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908631.1"
                     /translation="MSQVTERVLLEETNIKITSVKAIIVSKTHMLVNTTSVNLTVRHD
                     KGLLPLILILLGAVIGIIFVVSSITP"
     gene            5113..5196
                     /locus_tag="VFQ23_18430"
     tRNA            5113..5196
                     /locus_tag="VFQ23_18430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5147..5149,aa:Leu,seq:taa)
     gene            5710..>5951
                     /locus_tag="VFQ23_18435"
     CDS             5710..>5951
                     /locus_tag="VFQ23_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908632.1"
                     /translation="MVKRRKKKHIRSTPERETKTKAMKALWQTNHSRNSLTEFWRDYL
                     QDLATEKPGDEQKNSPVEQNTAKAQDAVHQELFSSD"
BASE COUNT         1590 a         1430 c         1329 g         1602 t
ORIGIN      
        1 ccgcggctca ggcaagcaaa gcgacaggcg cggtcatagc cagccataca atcggcggaa
       61 aagttgccaa ggaagagatg gacgttctgg aagcggctgg gcacgattta caacgattta
      121 tctggatcca tgcccaatca gagccagacg tgaccgtgtt gaaagaggca gcggcgcgcg
      181 gcgcatacat cgaattggat tctataggag cgccaaatca atcggaggca gaattggtcg
      241 atacgatcct ggctcttatg gaagcaggtt atcttgatca tattttgcta tctcacgatg
      301 caggttggta taacccgggt cgacccgatg gaatgccaga tcatggattc cgcggttata
      361 cggcactgac aaaggaattt ctcccggcag tatctgcgcg gggaatcagc gacgaactga
      421 tacagcacat cacgatgagc aacccagctc gcgcctttgc cttctagatc tcaataattc
      481 cagaaattta ctgtttgaca tgtcagaact cgctacgagt gattcccatt agtcactcta
      541 agggataatc cccattttag ttttgattgt ttcattgtaa attttcatac atgaatacaa
      601 tcgaaatgat ttggcgtggt tctgttctgg tggttctcgg atattttgcc tggagtagtt
      661 ttttggcggg aagtatcctg gctgttttca tcttgttgtt ctgtatcccg gtctggttgt
      721 ttattgggtt gtacaacatc ggatacttct agtgagacga tgcagattga ggggtcgagt
      781 tcaatgtcca ctaaatgaga atcgcgtgtt actgtgtttc ctgtaaccaa tttgggggag
      841 attccccatt ttatagttcc tcagttcatt gtaaattaga taggacagta tcaaaggaga
      901 accgatgaac agtcttaatc tttccactca atcgtatttt tattggctcc cacccgttac
      961 cttaacagat acgaattcgt tagataagtc tggcacatat gcagcctgcg agcaaatgat
     1021 ccagatgcct ttcatgatga tgggcttatc caatctggat gaaatgctcg agaggctcga
     1081 gaaaaacggc cgtctcagcg ccaaggaaca tcaaagcctg gtcgaacttg ccagaaagac
     1141 atggcgcgtt aattcttgag ttttcccatt gagatctaaa aacaattcta catataaaaa
     1201 tagacctctg actcaaaata gtttcagggg tctattttca tgtactgtga gcgttaatga
     1261 tcgttctcac ccgaatccaa ataattcgaa aggatatata taataaatga atattgaaca
     1321 acttcctgag ccccgcgttg aaattttcag tgaacctagt ttcctggaga aagctgccaa
     1381 ttttttagag tctgatgtag tcatctttga tagctcaact gggtacgaga ggcatggagt
     1441 ggatggatat atccaatatc tgacagaatt cgtccaaatc attcctgacc ttacaagcaa
     1501 ggtaatcgat catcaaacat acgacgataa attgattagc cgccttcaga tgcagggcaa
     1561 atttactggc actctgcgaa ctgacgaagg catatttttt ggaaatggaa atccgataga
     1621 tattgaatat caggttgagc aggaattcaa tcaccttggc aaagtcagac gattggtctt
     1681 caattatgat ttgcccgaat tcctctttca gctaagccgc agatatcgag cctcatgacc
     1741 gtaaatcggc aagctaaact tcgcaccccc attcatcatc aagttaaata aaatgccccg
     1801 tcagagtgag gggggcaccc tgatcggagc aacgttattt tcacacagat acttattttt
     1861 tgcaagggtt agcataatgg ggattaaccc taccttacct atgggggcaa tgcctgcaca
     1921 ttgcgggagt ggcgaattta gtgtgtaagc cacacacctg cctgatttgc tcgagcagat
     1981 cacgggcggc gctagggcag cccacagagt ttttgagttt ttttccacct gccccgaacg
     2041 caggtgcggg gtcaaagagc tcagtgctct ctgtggctaa gtggattttc actcactctc
     2101 ttagctactc cccacattgc aattctatat caaaaagtaa aattggtcgt ataatcgatt
     2161 tatcccatcc ggaaagacat ttacccgcta cacctgaacg tgaacggtac gctgactgtg
     2221 atttgtgcgg gaggaataaa gtaggttctt agttcatatt gcgaaaattc agatgaaggg
     2281 tttgcagcat ggcaaaagct gaggctccac aactaaacag cattgtggcg caaagagaaa
     2341 cattagcatc tccagaagtg acccggaaaa attatgtgga ccttctgtgg atagtggcag
     2401 cgggcaccct gggtgtcggt ctgctgtgtg ttactgccgg aattgctctt gcaaacccaa
     2461 gtacctctgc catgttgcag cctcccagca ttattcagat cgcgatcgcc aatgtcagca
     2521 ctgctgcggc gttaacgatg gagtcgttcc ctcagcattt gatcacgcct accatcagcc
     2581 cgtcggcttc gagtacggcg ttgccaggta cggtcaccgc atctccttct gttacacccg
     2641 agtcagcgac ggatactgcc acctccacat tggtcccaat gacgagtaca aatcggccaa
     2701 ggcgagggtc caattcgaat ccatttgcgt caaatacacc gacaaccata cctacaagaa
     2761 ccagttcccc cgtacctcca acactcacca atactccggt aaccccgact attacaaata
     2821 caccggaacc gactcataca ccgagaccaa cgagaacacg cacacctgtg ccatcaccaa
     2881 cggatacttc gccacctcct ccaacggata cctcggttcc tgttccaacc gacacatctg
     2941 cacccgcgcc aacgaacacc tcggcgcccc ttcctacgga tacatctgca cctgctccga
     3001 ccgacacatc ggctcctccg gctacagata catctgagcc ccctccgaca aatacactta
     3061 ccagtccgtg aggtctgagc ggcagtaacc gccgctcaac tagcgccttc gccgcgtgga
     3121 caaatgtgat aaccattgca acgtttgacc aaagacttga tcaactcatc atgcctgatt
     3181 tgggcttggc gcatctctgt gagatgcagt tcccaaagag gagtgccatc ctccaagtca
     3241 gttacacaga aaattaatcg ttccactagg caattcacac atcaacgaat tcttacttag
     3301 tcaatggcag gttcattaac cacgctttgg gcgaggaagg cgtatcacta catgtgtaca
     3361 gtagcgacac tttattctac cgttttgcca attgaaagtt tgtgtcagtt tagctggttt
     3421 ttaatagcac ggtagtaaat agaggatatc tcgcacaaag ctgttgtaat caaaatgtcg
     3481 gatcaaaatg atgggatgga gtggtccgga gtgcccaaaa gaaaatctct cctcaaaggg
     3541 agagatttgt tttatccttt tcgatgtaaa attattgcat gctccaattc ctcgaggaaa
     3601 attatcaggg tcccgcattt gaattatggg gaataacaca cctcggagcg attggtgtca
     3661 tcatcgtcct aaaccttctc ctcataagct ccaaaaatgc cagcgacggt acgaagactt
     3721 ccattcgttg gctgcttgct ttgatattga tagcgaatga aattgcctgg cattactgga
     3781 acgttgctta tgacaattgg accattcaaa caatgctgcc tctgcacttg tgcagtttgc
     3841 ttgtgtggct tggcgcactg atgcttgtta ccaggagtta cagaatttac gaattcatgt
     3901 atttcatggg tataggcgga gccatacagg ccctggcgac gcccgggatc ggtatttatg
     3961 gtttcccaca ctttattttc ttccaatatt tcatctcaca tggattgatc atcacatcag
     4021 ccatttatat gacaaccgtg atcggtcttc gccccacctg gaagtccatc ctgcgcgtgg
     4081 taatttggat gaatatctac gtggtgatta tctacttcgt caatgtggcg attggcagca
     4141 attacctgat gctcttggag aagccatcta cgccgagcct gcttgaccgg cttccagaat
     4201 ggccgcttta tattctatac atggaaggca tcggccttgt cacttgtata ttgctctacc
     4261 ttccattcgc aatcaaagac tggcgacaca aatatttgat gaacaaggac aatgcttcac
     4321 gtctggaaag tatttcaaaa taagcaaatt ttcatatgga tagattttag atcctttttg
     4381 tttttccccc ggcctaagcg tttatatcat cctttacggc caatcgctct atttatcgca
     4441 tccacgatcc ttctgatctg gttttggtta taggattgca ggatgtctgt ttccccggat
     4501 gaaccctcta gtttgattat gtacagaggc ttggaccgca cgtgaatgaa atgccccagt
     4561 cccaaaaaag ctacagcaac tatcccgccg ataatcagca atccccaatt gataatacct
     4621 tcagggggtg atcgagctta cgacgaagat aatcccgatg actgctccca aaaggataag
     4681 gattaatggt aacaatcctt tatcatggcg cactgtcagg tttacagaag ttgtattcac
     4741 cagcatatgg gttttagata caatgattgc cttcacgctg gtgattttta tattagtttc
     4801 ttccagaagt actctctcgg tgacctggct catagaatcc tcgttgctct atagtatcag
     4861 ctcgttgtat gagaattggg gtaatgctca cattatcata atcctaccca gatctaccac
     4921 cccttgcatt cgcctcatcg gtcgaaggcc ggaatcctgt gattctaaat catgtagcca
     4981 aatttgtgtg agtgtcagtc gagaatcaca ggatttgcaa tttagcggct agcgtcggtc
     5041 ggtatacatg aaaagcgact tgtacttttc aatcaacagc cttttgattt gcggtaaaat
     5101 gtagtgcacc ctggggcgat ggcggaatcg gcagacgcaa cagacttaaa atctgtcggt
     5161 ggcgacaccg tgagggttcg accccctctc gccccacaga agcccgtgtt tctctgattt
     5221 tccggaggag cgcgggtttg tgttttttgt ggagcatgca aaatattcct tctaactgag
     5281 ccaatttcgt aaactacctg taaggtgcaa agacacaaag cccctgtata ctaactttac
     5341 actttctagt cagagtgctg atttattgcc ctcccccagg tccacaccca cctgggggtt
     5401 gtgctttaaa agcacccggc cccagtgtgc tggggccggg tcggtacaga tgctggtctg
     5461 tgcctgaatt tcatctttca tactgaggag agagttggaa caaccgtttg tcttattgca
     5521 ttgtatgctt ttgaaatccg cgtgacaatt agggaagtcc cccattatgt aacctcacgc
     5581 aatgttctct caattgtcgc gcacaataaa ctgtgtaata tatagacagc aatccaaaat
     5641 atttattctc gaagttggta gcacatccct ctaggttcat tgtccagggg gatgcacaat
     5701 ttatatgcca tggtaaaacg aagaaaaaag aaacatattc gaagcacacc cgagcgcgag
     5761 acaaagacaa aggcaatgaa agccctttgg cagacaaacc actctcgaaa ctcgttgacg
     5821 gaattctggc gcgactatct gcaggatctg gcgactgaaa aaccggggga tgagcaaaaa
     5881 aattccccag tcgagcagaa tacagcgaaa gctcaggatg cagtgcacca agaattattc
     5941 tcatcagatg a
//
LOCUS       DASTJP010000626         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_927876, whole genome shotgun sequence.
ACCESSION   DASTJP010000626 DASTJP010000000
VERSION     DASTJP010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927876"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VFQ23_18440"
     CDS             <1..1484
                     /locus_tag="VFQ23_18440"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9908633.1"
                     /translation="AMTFHSTQDEIERGRALFEANCSNCSTDYFRDPSKMSSLSEVEL
                     ARIIKEGNAEVQAFGENFDEEEVWAVAAYLRTLSFSDSSMVSASTPLASQNEPTSVSS
                     TPVSAEETPAANATQAPVPGETQPGYGTVTGSIENETGVDLPTNLTITLRGFDHGTDP
                     NSTPDEVLSLEGNLDENGTFTFETVEMPASRIFLAQTTHEGITFQSEFGVVVEGASLL
                     TLSPIRLYGTTDDPSAIVIDDARIFFDYADNAIQVYGVYSFRNVSDKALTVELNDGVE
                     IPFIKSPDGAQPLGFEPTQDSQPFSSINNKLAFPPSEEPYGLIVFASMQKGDPVDVTH
                     SFDLPVASLTVFLPAGVSAEGSQLTDHGVQTMEGLNFQMYAAGDLPAGNTLTFTLSGT
                     PSDSTTPSSSPVSETGSNRNILIGAGVLGLVLIAAGAWMFVRDRKPTEGLNKKEQDND
                     FDTPDEVMDAIIALDDLHHAKRISHQAYQKRRNELKDILKEMI"
     gene            1518..2216
                     /gene="ccmA"
                     /locus_tag="VFQ23_18445"
     CDS             1518..2216
                     /gene="ccmA"
                     /locus_tag="VFQ23_18445"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HET9908634.1"
                     /translation="MILVKKLVKRFGLKTILRGVDFQVQPGEFVALLGPNGAGKTTFL
                     RILASLSRPSLGEVKIAGYKLPDQATQVRARLGVVSHLPLLYSDLSAEENLRFYARLY
                     GIANYRDRIFEILEMVGLTVRRQDLVRTFSRGMQQRLAIGRAILHDPDVMLFDEPYTG
                     LDQDASEVLDEILKNVAGQGRTVVMTSHDLSRVESLATRFDILSRGVITASATRKQIK
                     KSNLLTFYRQALLA"
     gene            2237..>2741
                     /locus_tag="VFQ23_18450"
     CDS             2237..>2741
                     /locus_tag="VFQ23_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET9908635.1"
                     /translation="MDAHSTPTPSLLRAISAIVWKDLRAEFRSFELVSAMLVFSLLVI
                     LIFNFALELDIKTRQSVTAGVLWATFAFAGTLGLNRSMAIEKDRGSFDGLLLAPVDRS
                     AIYFGKFISNLAFMLLVEIIVLPIYSILYNTNLFQPGLLMVILLGSIGYTAVGTLLAA
                     MSVQTRTR"
BASE COUNT          669 a          682 c          678 g          712 t
ORIGIN      
        1 acgcgatgac cttccatagc acgcaagacg aaattgaaag aggcagagct ctctttgaag
       61 caaattgctc gaattgttcg acggattatt ttagagatcc atccaagatg tcgtcattga
      121 gcgaggttga gttagcgcgc atcataaaag agggaaatgc tgaagtgcag gcgttcggag
      181 aaaatttcga tgaagaggaa gtctgggcgg ttgctgccta tttgcgaacc ctgtcatttt
      241 cagattcatc catggtttct gcctccacac cactggcttc gcagaatgaa cctacttccg
      301 tttcttccac tcctgtctcc gctgaagaga ctcctgcggc taacgcaacg caagctccgg
      361 tccctggtga aacgcaaccc gggtatggca ccgtcaccgg atcaatagaa aatgaaaccg
      421 gtgtagattt accgaccaac ttgacaatca ccttacgcgg ctttgatcat gggacggatc
      481 caaactcaac tccggacgag gttctcagcc tcgagggtaa tctggacgag aatggtacgt
      541 tcacttttga aactgttgaa atgccggcca gccgcatctt tctcgcgcaa accactcatg
      601 agggtattac atttcaatcg gagtttggag tggtggtaga aggcgcgtcg ctgctgacgt
      661 tgtcaccgat ccgattgtat ggaacgaccg atgatccatc ggcaatcgtg atcgatgatg
      721 cgcgcatctt ctttgattat gcagacaatg ccattcaggt ctatggagtt tattcgttcc
      781 gcaacgtatc agataaagcc ctgaccgtcg aattgaatga tggggtcgaa atcccattta
      841 tcaaatctcc agacggcgct caaccgctgg gctttgaacc cacgcaggac agccagcctt
      901 tttcgtcgat taacaacaag cttgccttcc cacccagcga agagccttat ggtttgatcg
      961 tattcgcctc catgcaaaaa ggagacccgg tggacgtgac tcattccttt gatttacctg
     1021 ttgcttctct taccgttttt ttacctgcgg gcgtgagcgc agagggatcg cagttaaccg
     1081 atcatggcgt gcagacaatg gaaggtctta atttccaaat gtacgcagcg ggagatttgc
     1141 cagcagggaa tacattgacg ttcactcttt cgggcacacc gagcgattca accaccccat
     1201 catcgagccc ggtttctgaa acaggatcca atagaaatat cctgatagga gcaggagtgc
     1261 tgggtctcgt gctgatcgct gctggagcgt ggatgtttgt gcgtgaccgc aaacccaccg
     1321 aaggattgaa taagaaagag caagataatg attttgacac gcctgacgaa gtgatggatg
     1381 caatcattgc actggatgat ctgcatcatg caaagcgaat ttcccatcag gcatatcaga
     1441 aacgtcgaaa tgaactgaaa gatattctaa aggaaatgat ctaactccgt cgggacaaat
     1501 tccctattaa gtcaaatatg atcctcgtca aaaaacttgt caagcggttt gggttaaaga
     1561 ccatcttgcg cggtgtggat tttcaagtgc agcctggtga gttcgtggca ttgctcggcc
     1621 cgaatggtgc tggtaaaaca accttcttaa gaattctggc ctctttgtcg cggccctcgt
     1681 tgggtgaagt gaaaatcgcc ggatataaat tgccagatca agccacgcaa gtgcgcgcaa
     1741 ggcttggcgt agtctcacac cttcccctgt tatacagtga cttaagtgcg gaggaaaatc
     1801 tacgcttcta cgcgcgctta tatggaattg cgaattaccg ggatcgcatt tttgaaattc
     1861 tcgaaatggt cggactcaca gttcgccgcc aggacctggt ccgtacattt tcgcgcggca
     1921 tgcaacagag gttggcgatt ggacgcgcca tcctccatga cccggacgtt atgctgttcg
     1981 acgaacccta cacaggcttg gatcaggacg catccgaagt gctggatgaa atcctgaaga
     2041 atgttgccgg gcagggacgc accgttgtga tgacgtctca cgatctttcc cgcgtggaat
     2101 ctctggccac ccgtttcgat atattgtcgc gtggtgtgat cacagcctca gcaactcgaa
     2161 agcaaataaa gaaaagcaat ctcctgacat tttatagaca ggctcttctc gcttaacgtc
     2221 cgctccacac taccatatgg atgctcattc cacgccgacc ccatcgctcc tccgagcaat
     2281 ttccgccatc gtatggaaag atttgcgcgc agaatttcgt tcgtttgaat tggtttccgc
     2341 gatgctggtc ttttctcttt tagtgatctt aatcttcaat tttgcgctgg aattggatat
     2401 taaaacccgt caatcggtga cggccggtgt gctctgggca acatttgcct ttgcgggaac
     2461 gctcggcttg aatcgctcaa tggctattga aaaggaccgc ggatccttcg atggtttgtt
     2521 gcttgcgcca gtcgatcggt ccgcaattta tttcggcaaa tttatcagca acctggcctt
     2581 catgctgctg gtagagatca tcgtcctgcc gatatatagt attctctaca atacgaattt
     2641 attccagccc gggttattga tggtgatcct gcttggttcc atcggatata cagccgtggg
     2701 taccttgctg gccgccatgt ctgtccaaac gcgtacacga g
//
LOCUS       DASTJP010000627         4867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_684859, whole genome shotgun sequence.
ACCESSION   DASTJP010000627 DASTJP010000000
VERSION     DASTJP010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684859"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /gene="sthA"
                     /locus_tag="VFQ23_18455"
     CDS             <1..1408
                     /gene="sthA"
                     /locus_tag="VFQ23_18455"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HET9908636.1"
                     /translation="HMGEYDYDLLVIGSGPAGQRAAIQAAKLQKHVAIVERRTVIGGV
                     CINTGTIPSKTLREAVMHLSGYRERGIYGASYAVKQNITMDDLLFRADHVMRHEIDVT
                     RHQLMRNRVDLLAAEASFVDPHTIRINYLDGRGQRLVTAANVVIATGTAATKDNRVPL
                     DGQHIFLSDDIIHLSNLPRSLAVVGAGVIGLEYASMFAALGVRVTLIDKRNRLLPFVD
                     GEIIEALEYHLRQNRVTIRLGEEVSGLEPIEDEKGSRVRICLTSGKQIITEQALYSIG
                     RTGATATLNLPDAGVNADARGYLKVNEHYQTETPHIYAVGDVIGFPSLASTSMEQGRL
                     AACHAFGVEAKSVPELFPYGIYAVPEISMVGKTEEDLTHQGVPYEVGKANYREIARGQ
                     IIGDSTGLLKLIFHIETHELLGVHILGEGASELIHIGQAVMAFGGPVDYFINTVFNYP
                     TLAECYKTAAFDGINRLG"
     gene            1465..2067
                     /locus_tag="VFQ23_18460"
     CDS             1465..2067
                     /locus_tag="VFQ23_18460"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9908637.1"
                     /translation="MVARTFVIAIAGHSGAGKSTVIENLVSQLGNANSLSLDEYESSS
                     IYPSTMKWLEDGANPNEFQSVQFVADITTLKNGNGILHPITGKKIEAASFLIVEEPFG
                     RERKALHDLIDLVVYIDVPLEVAYARKLARKNEFLPWEDDPDTFISNLRGNLEWFLGS
                     GREFYLALARRVQPNCDLILDGTLPVESITSQILAVIEKR"
     gene            2205..2657
                     /gene="msrB"
                     /locus_tag="VFQ23_18465"
     CDS             2205..2657
                     /gene="msrB"
                     /locus_tag="VFQ23_18465"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET9908638.1"
                     /translation="MKTFTKPSDNELRERLSRLQYEVTQHEGTERPYSNEYWNNKQPG
                     IYVDIVSGEPLFSSLDKYDSGTGWPSFTKPLDAENIATHTDYKLFLPRTEVRSKHADS
                     HLGHVFDDGPAPTGLRYCMNSASLRFIPAERLEEEGYGEYQSLFKDQK"
     gene            complement(2716..4227)
                     /gene="malQ"
                     /locus_tag="VFQ23_18470"
     CDS             complement(2716..4227)
                     /gene="malQ"
                     /locus_tag="VFQ23_18470"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET9908639.1"
                     /translation="MKFERSGGILLHPTSLPGPYGVGDLGPQAYQFVDWLESTGCKLW
                     QILPLGPTGYGDSPYQCFSAFAGNPYLISFDALVEDGLLTKDDFAEMPEFSDSRVDFG
                     RLIPWKLNLIERAYSRFVHNNNQESLSDEFNYFLAENASWLDDYALFMSLKEANGGGA
                     WNNWDASIRQREASAMDKARKDHAESVRRHSFYQFLFFRQWNKLRSYANERNIKIIGD
                     IPIFIAYDSADAWANPELFFLDEASLPTVVAGVPPDYFSPTGQLWGNPLYRWDVHKET
                     GYAWWVERFRAVLKMVDIVRLDHFRGFAGYFEIQFGQPTAEHGRWVPGPGMDFFTAVD
                     ARLSDGLSTPQIDLPIIAEDLGVITADVVALRDEFRLPGMKILQFGFATPKDPFLPHN
                     YVAKCVAYTGTHDNDTTLGWFDSAPEEERSFALRYLNSNGSDFAWDLIRGVWSSVAVY
                     AITPMQDLLSLGTEARMNYPSRLGGNWAWRMKEEDMSIPLARRLHEFNELYLR"
     gene            complement(4217..>4867)
                     /locus_tag="VFQ23_18475"
     CDS             complement(4217..>4867)
                     /locus_tag="VFQ23_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9908640.1"
                     /translation="WEDRQMDGSSHDFGKDILPRLIKSKARVFAFPYSGYWMDVGTMY
                     SYWQAHMDMLSPAPPLKLHSRSWVIHTRSEERPPARLPATAHVYASMLCDGCYIEAGA
                     RVESSVLSPGAVVRSGAVVRESIILTDGEIAGGAVVERAIIDKYVRVGENARVGGGVA
                     SANVKIALVGKNSVVPPNYIIEAGAEVGTDVTESDYPSSIVRDGEIIRKGRPSNEI"
BASE COUNT         1267 a         1262 c         1196 g         1142 t
ORIGIN      
        1 gcacatgggc gaatatgact atgacctgct cgtgatcggc tcaggacccg caggtcagcg
       61 ggcagccatt caggcagcaa agctccagaa gcatgttgcc atcgtcgaac gccgcacggt
      121 aattggaggg gtttgtataa ataccggcac cattcccagt aaaaccttac gtgaagcagt
      181 catgcacctc tctggctatc gcgaacgcgg aatctatggt gcgtcgtacg cggttaaaca
      241 gaacattacc atggacgatc tgctgttccg ggcagaccat gtcatgcgtc acgaaatcga
      301 tgtcacgcgt catcagctga tgcgcaaccg cgtagacctg ctcgctgccg aagcatcgtt
      361 tgttgacccg cacaccattc gtatcaacta tttggatggg cgtgggcagc gcctggtgac
      421 cgcagctaat gtcgtcattg ccacgggaac cgctgccacc aaagataacc gcgtcccgct
      481 ggatggacaa cacatctttt tgagcgacga cattatccac ctaagcaatt tgccgcgctc
      541 gctggcggtg gtcggcgcag gcgtgatcgg tctcgaatat gccagcatgt ttgcggcact
      601 gggcgttcga gttacgctta tcgataaacg gaatcgcctg cttccgtttg tggacggtga
      661 aattatcgag gcgcttgagt atcatcttcg ccagaaccga gtcacaatca gactcggcga
      721 agaggtaagc ggactggagc cgattgaaga tgagaaaggc tcacgggtac ggatttgcct
      781 caccagcggt aaacagatca tcactgaaca ggctctgtat agcattggac gtacaggtgc
      841 gacagccaca ctcaatctgc cagatgcggg agtgaacgcc gatgcgcgtg gctatctcaa
      901 agtgaatgaa cactatcaaa cggaaacgcc tcatatctac gcggttggcg atgtgatcgg
      961 attccccagc ctcgcatcca cttccatgga acagggtcgg ctggcggcct gtcacgcgtt
     1021 tggggtcgaa gcgaaaagtg tccccgaatt attcccttat ggaatttatg cggtgcccga
     1081 aatttcaatg gtcggaaaaa ctgaagaaga tctgacgcat caaggggttc cctatgaggt
     1141 gggcaaagcg aactatcgcg aaattgcgcg cggccagatc attggcgaca gcacaggctt
     1201 gctgaaactc atcttccaca ttgaaacgca tgaactactt ggcgtacata tcctgggcga
     1261 gggcgccagt gaattgattc atatcggtca ggcagtgatg gcctttggcg gacctgtgga
     1321 ttatttcatc aacacggtct tcaactaccc caccctggct gagtgctaca agaccgccgc
     1381 gtttgatggg atcaatcgtt tgggctaaga accagtattc tgctctgact acaacgtgat
     1441 taaagggcaa cagagtggag cgttatggta gcaaggactt ttgtaattgc tatcgcggga
     1501 cattcaggcg ctggaaagtc tacggtcatt gagaatctgg tctcccaatt ggggaacgcg
     1561 aactcgctca gtctcgatga atatgaatca tcgtcaatct atccatccac catgaaatgg
     1621 ctcgaagacg gcgccaaccc caatgagttt cagtcagtgc agtttgttgc ggatatcact
     1681 acactgaaaa acggaaacgg aatccttcac cctatcacag ggaagaaaat tgaggcggca
     1741 tctttcttga ttgtcgagga accatttggc agagagcgca aggcgcttca tgatttgatt
     1801 gaccttgtag tatatattga tgttccactg gaagttgcct atgcgcgcaa gctcgcgcga
     1861 aaaaacgagt ttctaccctg ggaggacgac cctgacactt ttatatcaaa cctgcgcggg
     1921 aacctagaat ggtttctagg cagcgggaga gaattttatt tggcgctggc acgaagagtt
     1981 caaccgaact gtgaccttat ccttgatggc acccttcctg tagaaagcat tacaagtcag
     2041 attcttgcag tcatcgaaaa aagatagaga aataaacctt cacctttgac ccatttctta
     2101 cataaacctt aacacagctc gtatacaggt cagtttttag gtctttctaa cgtcagaggg
     2161 atagaattca gaaacattaa tcgttttcac aatcggaggt ttatatgaaa acttttacca
     2221 agccgtcaga caatgaactg cgagagcgcc tttcgcgcct gcaatacgaa gtgacgcagc
     2281 acgagggcac tgagcgacct tattccaatg aatattggaa taataagcag cctggcatct
     2341 acgtagatat agtctcggga gagccattgt tcagctcgct cgacaaatat gactcaggca
     2401 caggctggcc cagctttaca aagcccctgg atgcagagaa tattgccacc cataccgact
     2461 acaagctctt ccttccacgt acagaggtgc gctcgaagca tgcagattcc catctcggtc
     2521 acgtcttcga tgatggtcca gccccaaccg ggctacgcta ttgtatgaat tctgcctcac
     2581 tgcgattcat cccggctgag cggcttgaag aggaaggtta cggagagtat cagtccttat
     2641 tcaaggatca aaagtaagtt ttgcgtaggc tccaattaaa aacagtctgc cggtatcggc
     2701 agactgtttt tttcgttatc gtagatacag ctcattaaac tcgtgcaatc gccttgcaag
     2761 aggtatgctc atatcttctt ctttcattct ccacgcccaa ttgcccccta atcgtgatgg
     2821 atagttcatg cgcgcttcag ttccaagact cagtaaatcc tgcatcggtg taatggcata
     2881 aaccgccact gacgaccata ctccacgtat gaggtcccag gcaaagtcgc ttccgtttga
     2941 atttagatat cgaagtgcaa aactgcgctc ctcttctggt gctgagtcaa accaacccag
     3001 tgtagtgtcg ttgtcgtgcg tacccgtgta cgctacacac ttcgccacat aattatgagg
     3061 cagaaatggg tctttgggag tggcaaaccc aaactgcagt atcttcatgc cgggcagtcg
     3121 aaactcatcc cgcaaggcta caacatcagc cgtaatcacg ccaagatctt ctgcgataat
     3181 tggtagatca atctgtggcg tagaaagtcc atcgcttagc cgcgcatcca ctgccgtaaa
     3241 aaaatccatt ccagggccgg gcacccagcg tccgtgttca gccgtaggtt gaccaaactg
     3301 aatctcaaaa tacccggcaa atccccgaaa gtgatccagt cgcacaatgt ccaccatttt
     3361 tagcacagcg cgaaaacgtt ccacccacca ggcatagcca gtttctttgt gaacgtccca
     3421 gcgataaaga ggatttcccc acaactgtcc ggtcggtgag aagtaatccg gtgggacgcc
     3481 agctacaacc gtgggcagac ttgcttcatc gagaaagaaa agttcagggt ttgcccaggc
     3541 atcggcgctg tcatatgcga taaagattgg aatgtcaccg ataatcttaa tgttgcgttc
     3601 gttggcataa gatcgtaatt tattccattg ccggaaaaag aggaattgat agaacgaatg
     3661 ccgcctgacg ctttcggcgt gatctttgcg cgctttgtcc atggctgacg cctcgcgttg
     3721 acgaatggaa gcatcccaat tattccacgc cccacccccg tttgcttctt tcagggacat
     3781 gaaaagtgca tagtcgtcca gccacgacgc attttccgcc agaaaatagt taaattcgtc
     3841 agatagcgat tcttggttgt tattgtggac aaaacgcgag taggcgcgtt cgatcagatt
     3901 caatttccat gggatgagcc ggccgaagtc cacacgtgag tcggaaaatt ctggcatctc
     3961 agcgaaatca tccttggtta gtaagccgtc ttcaaccaga gcatcgaagc tgatcagata
     4021 gggattgcct gcaaatgcag aaaaacattg atagggcgaa tcaccatatc ctgttggacc
     4081 caacggcaga atctgccaaa gcttgcaacc cgtggattcg agccaatcca caaattgata
     4141 cgcctgcggg ccaagatcgc caaccccata agggccaggc aagctggttg gatgaagaag
     4201 gattccacct gaacgttcaa atttcatttg aaggtctccc tttacggatg atttcgccat
     4261 cacgaacgat tgaggaggga taatcagatt ccgtcacatc ggtgcccact tccgcacccg
     4321 cctcgataat atagtttgga ggcacgacac tgttcttacc cacgagggca atttttacat
     4381 tggcggaagc gactccgccg ccaacccgtg cgttttctcc aactcggaca tacttgtcga
     4441 tgatggcgcg ttctacaacg gcgccaccgg caatctcgcc atcggtgagg ataatagact
     4501 cgcgcaccac agccccggaa cgaactacgg caccaggtga aagcacgctg ctttcgacgc
     4561 gcgcgcccgc ctcaatataa caaccatcac acagcatgct ggcatacaca tgagctgtcg
     4621 ctggaaggcg cgcgggaggt cgctcttcac ttcgtgtgtg gatgacccaa ctacgactgt
     4681 gcagtttcaa tggtggcgct ggcgaaagca tatccatatg tgcctgccaa taggaataca
     4741 tcgtacccac atccatccaa taaccactat agggaaaggc aaatacgcgc gctttggatt
     4801 ttatgagacg cggaagaata tcttttccaa aatcatgcga agaaccatcc atctgacgat
     4861 cttccca
//
LOCUS       DASTJP010000628         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_736757, whole genome shotgun sequence.
ACCESSION   DASTJP010000628 DASTJP010000000
VERSION     DASTJP010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736757"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFQ23_18480"
     CDS             <1..181
                     /locus_tag="VFQ23_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908641.1"
                     /translation="SFLLKPKIYEIDKISAKAEKVLGEDGVSLEELFETLREVRKELF
                     QERYGDVKKSSDEQS"
     gene            168..608
                     /locus_tag="VFQ23_18485"
     CDS             168..608
                     /locus_tag="VFQ23_18485"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET9908642.1"
                     /translation="MNKVNIFLDSSALIAGVLSTTGATHTLLIFGEDETILLTVSEWV
                     VRESEEALKRKSPKNIKALRTSLLTSKFQIVPDPSYAEIQANLYLMEDRDDIPVLLAA
                     IKAKVDYLATHDRKHFLDDPKVAERAGIKIGTPGDVLAWLRENL"
     gene            complement(768..1199)
                     /locus_tag="VFQ23_18490"
     CDS             complement(768..1199)
                     /locus_tag="VFQ23_18490"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9908643.1"
                     /translation="MAGFKMSEWISRPPQNVFDFITSPDNAPKVVHSVKSMVKLTDGP
                     VRVGTRYRETRLMRGKEEHAELDVVAYEPNQKYAVKNLTEGIETVYQYTFHPEANGTR
                     VDLECEVKAGGVKKLMLPLVVSILKKEDGDHLQRLKSVLET"
     gene            complement(1218..2174)
                     /locus_tag="VFQ23_18495"
     CDS             complement(1218..2174)
                     /locus_tag="VFQ23_18495"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9908644.1"
                     /translation="MIFTTNEKYEQAMLSTLIHIQTHLEGDLSLDLLAERVGFSAYHF
                     HRIFHEILGEPVKEYVRRLRVERAAYRLKTSEETILKIALDAGFKTHESFTRAFRRQF
                     GITPNQFRSNFLQASHTRKKQIQPKYISEFGVKNESGLLPNGSTSKKVRLEHVRPIVV
                     AFVRHVGPYDKLLDKGSSMSLLWEELFSWGNANQLINADSLLIGIPQDDPSITPPEKQ
                     RFDVCVQIPEFRNPSGHIGCQTISAGTFGVGRHYGSFENLADTYRHIYDALVTAGKFR
                     LRTATPFEVYSYSLIKGDIRIHFTDVFLPVESVETHRQTKGT"
BASE COUNT          657 a          575 c          571 g          668 t
ORIGIN      
        1 gtccttcctc ttaaagccca aaatctatga aatagataaa atttctgcca aggcagagaa
       61 ggtcttgggg gaagacggcg tcagtctcga agagttgttt gaaaccctga gggaggtgcg
      121 aaaagaattg tttcaagaac gctacgggga tgtgaagaag tcttccgatg aacaaagtta
      181 atatttttct ggatagcagt gctttaatcg ccggtgtttt gtctaccact ggtgccacac
      241 acacattgct catatttggt gaagatgaaa ctattttgtt gacagtaagc gaatgggtag
      301 tgcgcgaatc ggaagaggcc ttaaaacgga aatctccgaa aaacatcaaa gccttgcgta
      361 catctctttt aacttcaaaa tttcagattg ttcctgatcc gtcttatgca gaaatacagg
      421 cgaacttata tctaatggaa gatcgagatg atatacctgt gttgcttgca gcaataaaag
      481 ccaaggtgga ttacctagcc acgcatgatc gcaaacattt tcttgacgac ccaaaagtag
      541 ccgaaagagc agggataaaa attgggacac ctggcgatgt ccttgcatgg ttgagggaga
      601 atttgtaaca ggtaagacaa tccctcatcg aaatcgtctt gcttaatcta gtgctaagta
      661 caagcacgga gttcaatcgc ctttacatct aacaagcaaa ttgctcggtc caaaagctgg
      721 aatttcgttg tattgggtat tgtctgtcat tgcaacacga gaacctctca agtttccaga
      781 acgctcttta atctttgtag gtgatcgccg tcttctttct tcaatataga taccaccagg
      841 ggcagcatca acttctttac acctcccgcc ttgacctcac actccagatc cacccgcgtg
      901 ccgtttgctt cggggtgaaa tgtatattga tatacggttt cgatcccctc ggtcagattc
      961 ttcaccgcgt acttttgatt cggttcatag gcaacgacgt caagctcagc gtgttcctcc
     1021 ttgcctctca tcaggcgggt ctcacggtaa cgcgtcccca cccggacggg tccatcggtc
     1081 agcttgacca tgctctttac cgaatgaacc actttaggcg cattgtcggg cgaggtgata
     1141 aagtcaaaca cattctgagg tggacgggaa atccactcgc tcattttaaa tccagccatt
     1201 ttgttctcct ttttaaatta tgtccctttg gtttgtctat gggtttcaac agattcgaca
     1261 ggcagaaaga catccgtgaa atgaatgcgg atatcgccct taatcagcga gtaactgtac
     1321 acttcaaatg gggtcgccgt tcgcagcctg aatttaccag ccgtcaccag tgcgtcataa
     1381 atgtgcctgt aggtatctgc gaggttctca aatgaaccgt aatgccggcc gacgccgaag
     1441 gttccggcag agatcgtctg gcacccgata tgacctgatg gattgcgaaa ttcaggaatc
     1501 tgcacacaaa catcaaagcg ctgcttttcg ggcggtgtga tcgacgggtc atcctgaggg
     1561 atgccgatca acaacgaatc agcattgatc aattgatttg cattgcccca tgagaatagt
     1621 tcctcccaaa gcagagacat cgatgagcct ttatcgagca atttgtcgta tggaccgaca
     1681 tgtcgcacaa aagcgacaac gatcgggcga acgtgttcca ggcgaacttt tttcgaggtg
     1741 gaaccatttg gcagcaaccc cgattcgttt tttacgccga actcggaaat gtatttcggt
     1801 tgaatctgtt tttttcgtgt gtgggaggct tgcaaaaaat tactgcgaaa ttggttgggc
     1861 gtgattccaa actgccgccg aaaggcacgc gtgaaggatt cgtgggtttt gaatccagca
     1921 tccagtgcga tcttcaaaat ggtctcttcg ctggtcttca aacgatacgc ggctcgctcc
     1981 acccgcaggc ggcgtacata ctcttttacc ggttcgccca ggatctcatg gaaaatacga
     2041 tggaaatgat aagcagagaa cccaacacgt tccgccagca gatccagact taagtcacct
     2101 tcgaggtgag tctggatatg gataagtgtg gagagcatcg cctgctcata cttttcgttg
     2161 gtagtgaaaa tcattttgac tcgcgactca aactatacaa tagaaacggg atcgtttctt
     2221 gacttttttt gctattcctc tcggagtcgt ttgcccggta atctatggtt tagatttaat
     2281 aatgctagaa taagaaggct ttaagtagag ttcaatgaat cactttatcg aaattcgcac
     2341 cctcactatc aagccaaact cacgagaagg cttccaacaa ttattcaccg aacaagcatt
     2401 tcccctgctc caacgatgga agtttgatgt ggtggcacac ggtccgtcat tgcatgatga
     2461 gaatacctac t
//
LOCUS       DASTJP010000629         5163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1319075, whole genome shotgun sequence.
ACCESSION   DASTJP010000629 DASTJP010000000
VERSION     DASTJP010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319075"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..630
                     /locus_tag="VFQ23_18500"
     CDS             199..630
                     /locus_tag="VFQ23_18500"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9908645.1"
                     /translation="MSNDEIIIFLKKHLQSIQENDTKTYAETTAEDLTLYEWWITPHR
                     IDGLPFHEFMMTSNDERGAVFGSEGKAKSPTRFDLSNLHIQNYGDTVIASYTLLISTA
                     SPEGVKVASHNESRVMVKQNNSWKVVHVHKSPAWQAPHIPA"
     gene            627..2069
                     /locus_tag="VFQ23_18505"
     CDS             627..2069
                     /locus_tag="VFQ23_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908646.1"
                     /translation="MNLSVTRSYDFLWLSLALFVLIPVALFLSITPHDYWWYVRIGKD
                     ILETGSVPVMDTLSYTYPGRPVFYQPWLSAVLFWVAHSLGGATLTYLLKGLSIALAYG
                     LIWTLTRDAGTEPKIATLLVILMGISSSMNWSMRPQILAYPLFAVTLFALWHWQDRRD
                     KYLWLLPIASLLWANIHGSFILAFVLMGSALVLGAGDRKKLAIWLGISLLATFINPRG
                     VFVWQFVPDMLTSPSDQLYATEWQPPVNAGWQMNIFFGWFLLFIPLAAFSSRRLSLLE
                     WGWFLGFGWLALSGLRYVIWFMFIMTVLSGAVLTGLSERFKSKAGTETSKPTFNFVLG
                     GILLVLPFMMLPGIREAWWSDSPPPYQEATTPIAATQWLAEHPDLPGRLFAEYTFSSY
                     LTFALPSRLLWIDNRFNAYPPEHWSSYQTISSARAEWASLLAEDEINLLLLSLHSQPF
                     LVQAIEASDEWCEEYRDKDAVIFSRCEPIP"
     gene            2066..3154
                     /locus_tag="VFQ23_18510"
     CDS             2066..3154
                     /locus_tag="VFQ23_18510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9908647.1"
                     /translation="MKKFDGKLALQQRVLPTYRVPFFERLNTACEGGMSLYTGLPRPS
                     EGITTTDKIRISYYKPGKNIHLFGGKLYLCYQRELLQWLEEWNPDALIVEANSRYLAT
                     PAAVRWMKKQDKPVIGWGLGSPPVSGFRQQRRSAFLRQFDAMISYSQRGADEYAREGF
                     PLDNLFVAHNSVSPAPTWPMLDRSNTFNEKPCVLFVGRLQLRKNVDLLLGACAELESR
                     PRLIIVGDGPERDELEMLAEEIYPTAEFVGARHGAELKAYFTEADLFVLPGTGGLAVQ
                     EAMSYGLPVIVAQGDGTQDDLVREENGWQVLPDDLDALVATMNDALSDVARLRRMGEA
                     SYRIVKEEINIEKMVEVFVAALNSLGGK"
     gene            complement(3140..3541)
                     /locus_tag="VFQ23_18515"
     CDS             complement(3140..3541)
                     /locus_tag="VFQ23_18515"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9908648.1"
                     /translation="MSELNITTSQVQGEVSVVIFHLNGHLHGNTEQQLLDRARQSHED
                     GSKYVLLDLSDLEVLSSAGLRAIHSIFNMFTPQSDVEIIRQHRDEPYKSPYVKLVCPN
                     PDIYYILNITGFLQNLLIYTNMEEAVNSFAA"
     gene            complement(3668..>5163)
                     /locus_tag="VFQ23_18520"
     CDS             complement(3668..>5163)
                     /locus_tag="VFQ23_18520"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET9908649.1"
                     /translation="FVVGADAAVVRAAIMGSLALFAKQVGRRQFALNTLLAVAFIMTL
                     TNPLFVWDVGFQLSFFATLGLILYADPFTQFADRLLRKLFPASTAERVVELFSEFVLL
                     TLAAQLTTIPIMAYHFQRISLVSFIANPFILPAQPAVMILGGLSVMLSLVWFPLGQLM
                     AWVVWPFVVYTIRMVEIFDRVPHGTLFLGDLSIWFVILFYALLFFVTFGWGRLRAWSQ
                     AAGRDQVRVPAGSGLVVLALGLLLIWRVAVAIPDQLLHLTFLNVGSADAMLIQTPTGK
                     FILINGGPSVTTLSDELGRRVPAFNRKLDWLVVAATEEDQVAALPRVIERYPPEGVLW
                     SGNVEGSFSARVLDDFIAVQDIPVTLAKTDQVLDLGEGATLSVVATGPRGSILLLEYK
                     NFRGLLPIGMSFEALDELRNGASVGPVSILSLADSGYAPSNPPEWITNLNPELILLSV
                     EAGDRDGLPDTEVLETIEDYALLRTDESGWVEISTNGEQMWVNVEHQ"
BASE COUNT         1307 a         1302 c         1290 g         1264 t
ORIGIN      
        1 ggatgaatat ttgaaggtgt tgttgtaaga accggtggtt gagtagcccg gagcgttcgt
       61 tgcgaagggc atatcgaaac caccactaac taaaggtatt ctgttacagg gttaaacttg
      121 atggcacggt ggtttcgata cgggcgggaa aatcaccagc tctactcaag caccgaggtc
      181 gatagaaaag gagaagttat gtctaacgat gaaatcatca tattcctgaa aaagcatttg
      241 caatccatac aggagaacga taccaagacc tatgcagaaa ctactgcgga agacctgacc
      301 ctctacgagt ggtggattac accgcaccgc attgatggac ttccttttca cgaattcatg
      361 atgacttcca atgacgaacg cggagcagtg ttcggctcgg agggcaaggc gaaatctccc
      421 acccgattcg atctatccaa tttgcacatt caaaattatg gggatacagt cattgcaagt
      481 tatacattgt tgatcagcac ggcctcccct gaaggtgtaa aggtggcttc tcataacgag
      541 agtcgcgtga tggtgaagca aaacaattcc tggaaagtcg tacatgttca taaatcgcct
      601 gcgtggcagg cgccacacat cccggcatga atctttctgt aacgagaagc tacgattttc
      661 tgtggctgag cctggcattg ttcgtcctga tacccgttgc tctcttttta tctattacac
      721 ctcatgatta ctggtggtat gtgcggatcg gaaaggacat ccttgaaacg ggctccgtgc
      781 ctgtgatgga tacactcagc tacacctacc cgggtcgtcc tgttttttac cagccctggc
      841 tctcggctgt actcttctgg gttgcacata gcctgggagg cgcgacactc acttatcttt
      901 taaaaggact ctcgattgcc ctggcttatg ggttgatctg gaccctcacg cgagatgcag
      961 gtaccgaacc taagatcgcg acgcttcttg ttatccttat gggcatctcc agcagtatga
     1021 actggtccat gcggccgcag atcctggcct atccgttgtt tgctgtcact ttattcgcac
     1081 tttggcactg gcaggatcga cgcgacaaat acttatggct gttaccgatt gcaagcctgt
     1141 tatgggcgaa catacacggt tcttttatac tggcttttgt tctaatggga tccgctttgg
     1201 tattgggtgc gggtgaccga aaaaaacttg ccatctggct tggcatttct ttactggcga
     1261 cgttcattaa tccacgcggc gtattcgtct ggcaatttgt tcctgatatg ctgacatcac
     1321 cttcagatca gttatatgcc accgaatggc agccaccggt aaatgccggc tggcagatga
     1381 atatattttt cggctggttc ttgcttttta tccctctggc tgcattttct tcaaggcggt
     1441 tatctctgtt agagtggggc tggttccttg gctttggctg gctggctctc tctggactgc
     1501 gttatgtgat ctggtttatg ttcattatga cagtattaag cggggctgtg ctcacagggc
     1561 tatccgaacg attcaagagc aaggctggaa ctgaaacttc aaaaccgacg ttcaactttg
     1621 tgctcggcgg aatcctgctg gtgttaccat tcatgatgct ccccgggatt cgggaagcgt
     1681 ggtggagtga tagtccacct ccttatcagg aagccaccac gccgattgcg gcgactcaat
     1741 ggcttgcgga acatcctgat ctgccaggac gtctttttgc agaatacacc ttcagcagct
     1801 acctcacgtt cgcgctgcca tcccgcctct tgtggatcga taatcgcttc aatgcttacc
     1861 ctccggaaca ttggagtagc tatcagacga tatcctctgc ccgagcggag tgggcatctc
     1921 tgcttgctga ggatgaaatc aatttattac tgttatcatt acattcacag ccattcctcg
     1981 tgcaggcaat tgaggcttcc gatgagtggt gtgaagaata ccgcgacaaa gacgcagtca
     2041 ttttctcaag atgtgagcca atcccatgaa aaaatttgac ggcaaacttg ctctgcaaca
     2101 acgagtactg cctacgtacc gtgtgccttt ttttgaacgt ctcaatactg cctgtgaagg
     2161 tggtatgagt ttgtatacgg gcttgccgag accaagtgaa ggaatcacca ccacggacaa
     2221 aatacgcatc agctattaca agcctggtaa aaatatccat ctatttggtg gtaagcttta
     2281 tctctgttat caacgcgaac tcctacagtg gctcgaggag tggaatccgg acgcgctgat
     2341 cgtggaggca aattcacggt atcttgccac accagccgcc gtccgatgga tgaaaaaaca
     2401 agacaaacca gtcatcggct ggggattggg ttcaccgcct gtcagtggtt tccgtcagca
     2461 acgcagatca gctttcctca ggcaattcga cgcaatgatc tcctacagcc agcgcggggc
     2521 agatgaatac gcccgagagg ggttcccgct ggataacctc tttgtcgcac ataactccgt
     2581 ttctcccgcg cccacctggc cgatgctgga tcgttcaaac acctttaatg aaaaaccatg
     2641 cgttctattt gtaggtcgcc tgcaactccg taagaacgtg gatcttttgc tgggggcctg
     2701 tgccgaattg gaatcccggc cgcgactgat catcgtggga gatggaccgg agcgcgatga
     2761 acttgagatg cttgcggagg agatttatcc cacggcagaa tttgtcggag ccaggcatgg
     2821 ggctgagctc aaagcctatt tcacggaagc agacttattc gtcctgcctg gaactggcgg
     2881 tctcgctgtg caggaagcca tgagttatgg actacccgtc attgtggcgc agggagacgg
     2941 gactcaggac gatctggttc gagaagaaaa tggttggcag gtcctgcctg atgatcttga
     3001 tgccctggta gccacaatga acgacgcgct atcggatgta gcgcgtctgc gaaggatggg
     3061 cgaagcctcg tatcgaatcg tgaaagagga aatcaatatt gaaaaaatgg tcgaggtatt
     3121 tgtagccgcg ctgaactctc taggcggcaa atgaattgac tgcctcttcc atattggtgt
     3181 agatcaacag gttctgtaaa aatccggtga tgttcaatat atagtaaata tctgggttcg
     3241 ggcagaccag tttcacatag ggggatttat agggttcgtc gcggtgctga cggatgattt
     3301 ccacatcact ttgaggggta aacatgttga aaatgctatg aatggcacgc agccctgcac
     3361 tcgacaaaac ttccaggtca gataaatcca gcagaacata tttggaccca tcttcatggg
     3421 attgacgagc tcggtcaaga agctgttgct cggtgtttcc atggagatga ccgttcaagt
     3481 gaaagattac gactgatact tcaccttgaa cctgtgacgt agtgatatta agttcgctca
     3541 ttctccaact cctctagata aacttcttgt acttcccatg cagttcaatt gcaggtgagt
     3601 attcctgtcg aactgcggat aaaacggaca tttaaatata acacctgaga aatcagaaag
     3661 cgccgcccta ttggtgctcc acattcaccc acatctgttc tccattcgta gagatttcaa
     3721 cccaaccact ttcgtcggta cgaagcagtg cataatcttc tatcgtttcc aaaacctcgg
     3781 tatcgggtag tccgtctcgg tctccggctt ccacgctcaa taagatgagt tcggggttta
     3841 gattcgtgat ccattctggt ggatttgacg gtgcatatcc agagtcagcc agcgacaaaa
     3901 tgctcaccgg tccaacactg gctccatttc gcaattcatc taatgcttca aaactcatcc
     3961 cgatgggtaa tagccctcga aaattcttat attccaatag caatatggag ccgcggggtc
     4021 cggttgccac aacactcaat gtcgcacctt cgcctaaatc cagtacctgg tctgtcttgg
     4081 cgagcgtgac aggaatatcc tgcacagcaa tgaaatcatc caggacgcgc gcagagaagg
     4141 acccctcgac attgccgctc cacaacacac cttctggagg ataccgctcg atcacgcgcg
     4201 gcagtgccgc cacctggtct tcctccgtag cagcgacgac cagccaatct aactttcgat
     4261 tgaatgcggg cactcgtctt cccaattcat cagaaagcgt ggtcacactg ggtccgccgt
     4321 tgatcagaat gaactttccc gtgggcgttt gaatgagcat ggcatcggca gaaccgacat
     4381 tcaaaaaggt gagatgcaga agctgatcgg gaatcgcaac cgcgactcgc cagatcagta
     4441 acagacccaa tgcaagaacg acaagtccac ttcctgcggg aactcggacc tgatctcgcc
     4501 ctgcagcctg ggaccaggct cttaaacgac cccagccaaa cgtcacgaaa aagagcagcg
     4561 cgtaaaacag gatcacgaac caaatcgaga gatccccaag aaagagagtc ccatgcggca
     4621 cgcggtcaaa aatctccacc atccggatcg tataaacgac aaacggccat acaacccacg
     4681 ccatcaattg acccagcgga aaccagacca ggctgagcat aactgataat ccgcccagga
     4741 tcatcactgc aggctgtgca gggagaataa aagggttggc gataaacgag accagtgaaa
     4801 tgcgctggaa gtgataagcc atgatcggaa tggtcgttaa ctgagccgcc agggtgagca
     4861 ggacgaattc tgaaaagagt tcaacgacgc gctcggcagt ggatgccgga aataattttc
     4921 tgaggagtcg atctgcaaat tgcgtaaacg gatcagcata taagatgaga cccagcgtcg
     4981 caaaaaacga aagctgaaag ccgacgtccc aaacaaatag cgggttcgtc agagtcatta
     5041 tgaaagcgac tgcgagcagc gtgttcaatg caaactggcg gcgccctact tgcttcgcaa
     5101 ataatgccag gctgcccatg atcgcagccc gcactactgc ggcatctgca cccacaacga
     5161 acg
//
LOCUS       DASTJP010000630         4504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1096014, whole genome shotgun sequence.
ACCESSION   DASTJP010000630 DASTJP010000000
VERSION     DASTJP010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096014"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFQ23_18525"
     CDS             complement(<1..471)
                     /locus_tag="VFQ23_18525"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9908650.1"
                     /translation="MRISRFSGWLILIILFGMGGFVHPRPAQAEDIRPAPLQQVSAYE
                     LIIAMNTLRVSYGLPPLIEDPIVNAVAQSTAQIMAANNMSWHIGDVRGRIAAAGYGSG
                     GTVWATENFAVSSGGMGIDEIMAAWADPDHMRPAVEAAYCNVGAGVAQTSDGRIY"
     gene            607..1608
                     /locus_tag="VFQ23_18530"
     CDS             607..1608
                     /locus_tag="VFQ23_18530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9908651.1"
                     /translation="MKYRPLGKTDIQLSAVGLGCMGMSAQYGQADEGENIATLNRALE
                     LGINFWDTADVYGNGANEELVSKVLVQNREKIFIATKFGFRTREDQEDDTGVGEGYID
                     GSAAYVKQAVEASLRRLKIETIDLYYAHRVDPNTPIEETVGAMADLVKAGKVRYLGLS
                     ECSAESLRRAQSVHPISALQNEYSLLTRDIEKEMLPLTKELGVTLVAFSPLSRGLVTN
                     RLNVNSFEADDFRKHLPRYSGDYLENNQNLASEFAALAATKNITPAQLALAWVLAQSD
                     NIIPIPGTKRRKYLEENAGAADVVLANEEIEQIEALLKKYPNIGPRYSEQEFQFVNK"
     gene            1618..1878
                     /locus_tag="VFQ23_18535"
     CDS             1618..1878
                     /locus_tag="VFQ23_18535"
                     /inference="COORDINATES: protein motif:HMM:NF016855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET9908652.1"
                     /translation="MKNMGPKSSEWLASVGIHTLDDVAKLGVVEAYKRVKAAYPEKVT
                     LNMLWGLQAALLGLPWNELPSDIKEELRRQVDPLPSPPPNTP"
     gene            1996..2925
                     /locus_tag="VFQ23_18540"
     CDS             1996..2925
                     /locus_tag="VFQ23_18540"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HET9908653.1"
                     /translation="MLKPISRILNSLLLLVFIGAWGNILYHVKSAPRPAPMTTIDLVP
                     TAVRVPTSPVVPTSTVIPTPQTVHNLAATVEAGIPDGKWSQVQPGLERRVIPIYNSQN
                     QQVESMYVWRLDQKYFRLDVAFAERPKTLETWQAETNAAMVVNGGYFSINNERYSADG
                     LSIINGQASGRSFNGYGGMLAVDQFGAEVRWLVQEPYNASEPLHAALQAFPILVQPGG
                     ELGFPASRENYARARRTVIAQDRDGRIFLIVTPDGYFTLHQLSLYLTESDLDLDVALN
                     LDGGGSTGILVANPREIIAPRVLLPFVVLVHTR"
     gene            2947..4206
                     /locus_tag="VFQ23_18545"
     CDS             2947..4206
                     /locus_tag="VFQ23_18545"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET9908654.1"
                     /translation="MGKKSTGTVYPLSALRAMALHSQFLTTSNGSSAPAPTSNRIVDL
                     VKALGYIQIDTLNVVNRAHYATLWARFGSYDVKNFDKLIYKADKRKLYEGWGHAACII
                     PLEHYRYHRWRTDPKYSYNATFREWGNKEANRRLTDQTLARIRTEGALRVGDFEYDGP
                     QRGLWYDWKPPKMALEALFAWGDLMVSDRAVGFQRVYDVKERVLPEWVNATPVRPEEA
                     RRFCLEQAAKALGVFDLRHLTLYAYMKATPLRSEVQALVKDGTLVEIQGESMSGSKTW
                     MVHRDNLRLLQRAADGEITPQRTTFLAPFDSLFWAWKRDQMLWGFHQLLECYKPAAQR
                     VYGYFCFPILHKDRLVGRFDPKLDRKSGVLHLNALYLEPGIKPDDELVADIATTLRDF
                     LSWHGAKSLKIEKSDPPAFGKKLMNAL"
     gene            4319..>4504
                     /locus_tag="VFQ23_18550"
     CDS             4319..>4504
                     /locus_tag="VFQ23_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908655.1"
                     /translation="MKKRTQIRNSKIGQSGMNLVILILVFIVVGCAPAATQPVDQSIP
                     TPLIEATQVIEQATPTPI"
BASE COUNT         1135 a         1129 c         1188 g         1052 t
ORIGIN      
        1 atagatcctt ccatccgatg tctgagccac accggctccc acattgcaat acgccgcttc
       61 gaccgccggg cgcatgtgat ccggatccgc ccaggcggcc atgatctcat cgatgcccat
      121 gcctccactg ctcacggcaa agttttccgt tgcccagacg gttccgccgc taccataccc
      181 ggcagccgcg attcgaccgc gcacatcgcc aatgtgccag gacatattat tcgccgccat
      241 gatctgagcc gtcgactgag ccacggcgtt gacaatgggg tcttcaatga ggggtgggag
      301 accatacgaa acgcggagcg tgttcattgc gatgatcagt tcatacgcac tgacctgttg
      361 cagtggagcc gggcgtatat cctccgcctg ggcaggccgg ggatggacga agcctcccat
      421 tccgaacagg atgattagga tcagccaacc tgagaatcgt gaaattcgca tctgataatt
      481 ataattctgc acgccatgat aatctggagg agtggctcag gcgtagtttc tctacagatt
      541 ctcataaatt cacatgactt ggattcccgg actcctggtg atacactacg catggaggta
      601 aaaaatatga aatacagacc attaggcaaa acagatattc aactctccgc tgtcgggctg
      661 ggctgcatgg ggatgagcgc ccagtacggg caggccgatg aaggagaaaa catcgcgacc
      721 ttaaaccgcg ctctggaact gggcatcaac ttctgggata ctgccgatgt atacggaaac
      781 ggagccaatg aagagttagt ctcaaaggtg ctggttcaaa atcgggagaa aatttttatt
      841 gcgacgaaat ttggtttccg aacccgtgag gatcaggaag acgataccgg cgttggagag
      901 ggctacatag acggttccgc agcctatgta aaacaagcag tggaagcgag cctccgacgg
      961 ttgaaaattg aaaccattga cctctattac gcacatcgtg tggatccgaa tacgccgatc
     1021 gaagaaacgg taggtgcgat ggccgattta gtcaaagcag ggaaagtccg ttatttggga
     1081 ttaagcgaat gttcagccga atcgctgcga agagcacagt cggttcaccc gatctctgct
     1141 ttgcaaaatg aatactcgtt gctgacgcgt gatattgaaa aggaaatgct gccgttaacg
     1201 aaagaacttg gcgtcaccct ggttgcgttt tctccattaa gcagagggct ggtgaccaat
     1261 cgactcaatg taaacagctt cgaagccgat gatttccgaa aacatcttcc ccgttacagc
     1321 ggagattatc tggagaacaa ccagaatttg gcctctgagt ttgctgcgtt ggctgcaaca
     1381 aaaaatatta cgcccgcaca gcttgcttta gcgtgggtgc tggcgcagag tgataacatt
     1441 attcccatcc caggaacaaa gcgaagaaaa tatttggaag aaaatgcagg ggctgcggat
     1501 gtcgttttgg caaatgaaga gattgaacag atcgaggcgt tactaaagaa atatcccaat
     1561 atcggtcctc gctatagcga gcaggaattc caatttgtaa ataaataggc agtacgcatg
     1621 aaaaacatgg gtccaaaaag cagtgaatgg ctcgcctcgg tcggcattca cacattggat
     1681 gatgtagcaa agcttggcgt ggtggaggcc tataagcgtg tcaaagcagc ataccctgaa
     1741 aaggttaccc tcaatatgtt atggggactt caagccgcgc tgttgggttt accatggaac
     1801 gaattgccgt cggatatcaa ggaggagttg aggcggcagg tggaccccct ccccagccct
     1861 cccccaaata cgccatgagt agattagata ataatcctac cttcttagta gcgtatttgg
     1921 gggagggtgc ccatcagggc gggtgggggt agaacattaa ttctatttta ggctttttct
     1981 gctacaatgg cttttatgct aaagcctatt tcaagaatac tcaactcttt acttttactc
     2041 gtttttatag gcgcatgggg caacatcctg tatcacgtta agtctgcacc gcgccctgct
     2101 cctatgacga caattgattt ggtaccgact gctgtccgtg ttccgacatc tcctgtggtt
     2161 cccacatcca ccgtgatacc tacccctcag acggtccata acctcgcggc gacggttgag
     2221 gccgggatac cggatggaaa atggtcccag gtacagcccg ggcttgagcg gcgcgtgatt
     2281 ccgatctaca acagtcagaa ccaacaggtc gagtcaatgt atgtgtggcg actcgatcaa
     2341 aaatatttcc ggctggatgt agcttttgcc gaacgcccca agactctcga gacctggcag
     2401 gcagagacga atgccgcaat ggtagtgaat ggcggctact tcagtatcaa taacgagaga
     2461 tattcagccg acgggctgag catcatcaat gggcaagcct ccgggcgaag ctttaatgga
     2521 tacggaggta tgctggcggt cgatcagttt ggggcagagg tacggtggct ggtacaggaa
     2581 ccctacaatg catctgaacc cctgcatgct gcattgcagg catttcccat tttggtccaa
     2641 ccgggtggcg agcttggttt ccctgcctcc agagaaaatt acgcgagagc caggcgaact
     2701 gtcatcgcgc aggatcggga cggacgaatc tttcttatcg ttacgcccga tggatatttc
     2761 accttgcacc agctaagcct ctatttgacc gaatcagatc tggaccttga tgttgccctc
     2821 aatttagatg gaggaggctc cacagggata ttggtcgcga acccgcgcga gatcatcgct
     2881 ccaagagtat tacttccatt tgtcgttctg gtgcatactc gataaactct taaaagagaa
     2941 attcctatgg gaaaaaaatc taccggcacg gtctacccac tctctgccct ccgagccatg
     3001 gcgctgcata gccagtttct cacaacctcc aatggctcat cagcacctgc gccgacttca
     3061 aacagaattg ttgatctcgt gaaggcgctt ggctacatcc aaatcgatac gctgaacgtg
     3121 gtcaaccgtg cgcattacgc taccctgtgg gcgcgcttcg gatcgtatga tgtaaagaac
     3181 tttgataagc tgatctacaa ggcagacaaa cgcaagcttt acgaaggttg gggtcatgca
     3241 gcctgtatca tccccttgga acattatcgc tatcacagat ggcgaacgga tccgaagtac
     3301 agctataacg ctacctttcg tgaatgggga aacaaagaag ctaaccgcag gctgaccgat
     3361 cagacgttgg cgcgcattcg aacggagggt gctttacgtg tgggggattt tgagtatgac
     3421 ggtccacagc gcggcctatg gtatgactgg aaacctccaa aaatggcgct cgaggcgttg
     3481 ttcgcctggg gtgatctgat ggtgagtgat cgagccgtag gcttccagcg cgtttatgat
     3541 gtgaaggagc gcgtcctgcc agagtgggtc aatgccacgc ctgtccgccc tgaggaggcg
     3601 cgtcgcttct gcctcgagca ggcggcgaaa gcgcttggcg tgttcgatct tcgacatctt
     3661 actttgtatg cctacatgaa ggctacacct ctcagatctg aagtgcaggc gctggtgaaa
     3721 gatggaactt tggtggaaat tcagggtgaa tcgatgagcg gttctaaaac atggatggtg
     3781 caccgggata accttcgatt acttcagcgc gcggccgatg gtgagatcac ccctcagcgc
     3841 acgacttttc tagctccttt cgattcactg ttctgggcct ggaaacgtga tcaaatgttg
     3901 tggggctttc atcagctcct ggaatgctat aagcctgctg cacaacgtgt ctacggctac
     3961 ttctgcttcc caatcctgca caaggaccga ttggtgggcc gcttcgaccc aaagctggat
     4021 cgcaaatcgg gtgtgctcca tctgaatgct ctctaccttg agcctggcat caagccagat
     4081 gacgaacttg tagccgatat cgccaccacg ctgcgcgact tcctctcctg gcatggcgcg
     4141 aagagtctca agatcgagaa gagtgatccg ccggcgttcg gcaaaaaact gatgaatgca
     4201 ttgtagaagt ccagttcttc agtttctcac caaaacaact gctctcgcag tgacggatga
     4261 ttttggaaca aaaaggtgag gaaattcgtt cttctccccg tacagaggag taaagaaaat
     4321 gaagaaaaga acgcaaatta gaaactcaaa aatcggtcaa tcgggtatga atctggtcat
     4381 cctcatcctt gtgttcattg tggtgggatg cgctcccgcg gcgacacagc ctgtggatca
     4441 atctattccc acacctctta tcgaggcgac gcaggttata gagcaggcca caccgacgcc
     4501 catc
//
LOCUS       DASTJP010000631         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_550082, whole genome shotgun sequence.
ACCESSION   DASTJP010000631 DASTJP010000000
VERSION     DASTJP010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550082"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /locus_tag="VFQ23_18555"
     CDS             complement(<1..1606)
                     /locus_tag="VFQ23_18555"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9908656.1"
                     /translation="MTNGSSGQSGFEWENWAGNVKANPAYYFKPDTVDDLIAIVKQAN
                     SQTPKQKIRVVGSSHSWSALAQTDGYMVDLENFNWINIDRDTALVTVGAGALMMEVEA
                     QMKANGLAIPANAVTLMPRIGGLVAAGCHGSGYDYSIISDYVQAVSIVLASGEIQTFS
                     VDELGEDSDLMNAVRLNLGTFGIMYEIVLKAIPTFNVHCVDSTPDMKTTIDNIQDIVT
                     GNNYVEIFWFPFNPDNAIWLKTWNQIKPHEHEEVSGNAPKEYWERIEKRDFTTAELLK
                     AFEKMTANINFREAFKEAAELIRAVQPGTSGAVEVDDPKRHSTALSELLLCLGECAAA
                     GGNEEEIKKCMEGCLKEGGENVIGEELWAFIAEHPSLTPKILPLLWHLVPDRDDYVAE
                     VNDVMHFRRFFPKVALMAMAIPIDPNFENIKAAWNLTVQAVNELAASGQFPLNVNIEA
                     RFVKNSNCLISPINGSDHFCIIEVISYITTPTFNDFYAMIGQEWLKLGYNGIMPRPHW
                     PKQFDAIPNINQSIRQAYGDNLKKFLQ"
     gene            1833..2915
                     /locus_tag="VFQ23_18560"
     CDS             1833..2915
                     /locus_tag="VFQ23_18560"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9908657.1"
                     /translation="MAFKYVSLCMLALLISGCAESSTQIATPASTPLIVTATLPVTLA
                     PQATDSPLPPLPSPTVAPVEGITSSQVNVRAEPSTGSEVLGMIPPDMRVEIVGRDPGG
                     SWWQIIYPAGSPERGWVTAQFVRTVSTPEVPVIGGGPNPDDGNVAIIQEQLNVRSGPG
                     ADFNSLGTLNPQEVVRLTGKDANGGWLQIDFASGPEGKGWISAAFVQAQGVENLPIIA
                     ESGAVVGTGTPTEIPFTPTATLLPAPLDDDSAQTPAVTILLSPTGSQSFQYSSDVSSP
                     EGDGEDWIQFTPFTQAIRITLECVGNGTAILEVLENGSAIDSMECGENSVIDVTRGNQ
                     HTLSVQSNASNELGYTLYTLWVESIR"
     gene            3110..3727
                     /locus_tag="VFQ23_18565"
     CDS             3110..3727
                     /locus_tag="VFQ23_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET9908658.1"
                     /translation="MNTDKIPDIIIGRLPVYLRALQRLAEKGISTTSSQELGEIVGIS
                     AAQIRKDISQFGEFGKQGTGYAISYLVEKLQDILKVDRVWEVVIVGMGDVGHALAHYQ
                     GFADRGFHVKMVFDNDPKKIGDQFGEFQIYDTASLSEKIKQHKIKIAMIAVPASDAQT
                     VADQLVKAGVKAILNYAPISLTVPDGVRVQYIDPVTHLQRMTYYL"
     gene            complement(3804..4787)
                     /locus_tag="VFQ23_18570"
     CDS             complement(3804..4787)
                     /locus_tag="VFQ23_18570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HET9908659.1"
                     /translation="MQKVTTPQPPGKKILDQVTDAIRLKHYSYRTEQTYKEWIKRYIL
                     FHKKRHPAEMGAAEIQAFLSNLATEKKVAASTQNQALSAILFLYRNVLLKPMEVPTEL
                     IRAEKSKTLPTVLTHAEAMAVIHKLHGVPQIMTKILYGSGLRLIECLRLRVKDIDFGN
                     RQIIVRDGKGEEDRFTPLPETLIPDLQQHLQNVGYIHQKDLKAGFGEVYLPYALNRKY
                     QRAARDWVWQYIFPALSLSTDPISKKTMRHHADPSYLQKAIRQAAKSAGVQKLVSPHT
                     FRHSFATHLLQNGYDIRTVQELLGHKDVKTTMIYTHVLQKGGLAVKSPLDG"
BASE COUNT         1169 a         1180 c         1311 g         1314 t
ORIGIN      
        1 tttgcaggaa ttttttgagg ttgtcaccat acgcttgccg gatggactga ttaatattgg
       61 ggatggcatc gaactgcttg ggccaatgag gacgcggcat tatgccgttg tagcccagct
      121 tcagccattc ctgaccgatc atcgcataaa aatcgttgaa ggttggagtg gtgatgtagc
      181 tgatgacctc gatgatgcaa aagtgatcgc tgccattgat cggggagatc aggcagttgc
      241 tgttcttaac aaagcgcgct tcgatgttga cattgagtgg aaattgcccg ctcgcggcca
      301 actcatttac ggcttggacg gtcagattcc acgccgcctt gatattctcg aagttcggat
      361 cgatcgggat cgccatcgcc atcaaagcga ctttggggaa gaatcttcga aaatgcatga
      421 catcattgac ttcagcaacg taatcgtccc tatcaggaac taaatgccac aacaagggca
      481 atattttcgg ggtcaaactc ggatgttccg cgatgaatgc ccacaattct tcgccaatca
      541 cattttctcc cccctccttg aggcagcctt ccatgcactt cttaatttcc tcctcattcc
      601 cgccagccgc agcacattct cccagacaga gcagcagctc agagagggcc gtactgtggc
      661 gtttcggatc atcgacctcc actgcaccac tcgtgcctgg ctggaccgca cggatcaatt
      721 cggctgcttc tttgaaagcc tctctgaagt tgatattcgc tgtcatcttt tcgaacgctt
      781 tcagaagttc tgcagtcgtg aagtcgcgct tttcaatccg ctcccaatat tccttcggtg
      841 cgttaccact tacctcttca tgttcatgtg gctttatctg gttccatgtc ttgagccaga
      901 tggcattatc agggttaaag ggaaaccaga agatttccac gtaattattc ccggtcacaa
      961 tatcctgaat gttatcgatt gtggttttca tatcaggggt cgaatcgaca caatggacat
     1021 tgaaagttgg aattgccttc aacacgatct catacatgat tccaaacgtg ccaagattca
     1081 gacgtactgc gttcatgaga tcggagtctt cccccaactc gtcgactgaa aacgtctgga
     1141 tttcgccaga agccaggact atcgacaccg cctgaacata atcggagatg atcgagtaat
     1201 cgtagcctga gccatgacag cctgctgcga ccaacccacc gatgcgtggc atcagagtaa
     1261 cagcgttggc gggaatggcc agtccattcg ctttcatctg agcctccacc tccatcatca
     1321 aagcccctgc gccaacggtg actaaggctg tatcgcggtc gatattgatc caattaaaat
     1381 tctccagatc caccatgtat ccatccgtct gagccagcgc actccaggag tgactgctgc
     1441 caaccacacg aattttctgt ttgggtgttt gagagttggc ttgcttcacg atggcgatca
     1501 ggtcatctac tgtatcgggt ttaaagtaat atgccgggtt ggccttcaca ttccctgccc
     1561 aattttccca ctcaaatcca gattggccgc tgcttccgtt tgtcatgttg attgcctcct
     1621 aagataatgc accttggtct ggaaagttgg tttagcatat catattctcc aaaaggcgtc
     1681 cacattttaa gcaatccgga aggattaaga ggaggctagt cagcggcaat acccacccgc
     1741 aggcaaaatt caatgccgtt tgtttgaaat ccaggtgctg ccgcttcaca tgaaagtcag
     1801 ttgcggtttt tgataagcgg tagaatctcc ctgtggcttt caaatatgtt tcactttgca
     1861 tgctcgcact gctgatcagc ggttgtgctg aaagctcgac tcagatcgcc acacccgctt
     1921 ccactccatt gattgttaca gcaaccctgc ccgtgactct cgctccgcag gcaactgact
     1981 ctcccctgcc gcctctgcct tcaccgactg ttgctcctgt ggagggaatc acctcttctc
     2041 aggtgaatgt acgcgcagaa ccatctacag ggagtgaagt gctcggcatg atcccgccgg
     2101 atatgagagt tgaaattgtc ggcagggatc caggtggaag ctggtggcag attatttacc
     2161 ccgcgggctc accagaaaga ggctgggtga ccgcgcaatt tgttagaacg gtcagcacgc
     2221 cggaggtccc ggtgatagga gggggtccga atccggacga tggaaatgtt gcgattatcc
     2281 aggagcagct gaatgtgcgg agtggaccgg gtgccgattt taattcgctg gggaccttga
     2341 atcctcagga ggtggtcagg ctgaccggca aggatgcaaa tggcggctgg ctacagattg
     2401 acttcgccag tggaccggaa ggcaaaggct ggatcagcgc ggcattcgtg caggcacagg
     2461 gagtggagaa tttacccatt atcgcagaat caggcgcggt cgttggaaca ggaacgccga
     2521 cagagatccc gtttaccccc acagcgaccc tcctccccgc tccgttggat gacgattcgg
     2581 ctcaaactcc ggcagtgact atactgttgt ctcctacggg ctcgcagtct tttcaatact
     2641 ccagcgatgt ctcttctccg gagggagatg gcgaagattg gattcagttc acgcctttta
     2701 cacaagcgat tcgtatcacg ctcgaatgtg tcggaaatgg aactgccatt cttgaagtgc
     2761 tggaaaacgg ttctgccatt gatagtatgg aatgcggaga aaattcagtc attgacgtca
     2821 ctcgaggaaa ccagcataca ctttccgttc agagtaatgc ctccaatgaa ttggggtata
     2881 cgctttatac gctttgggta gagtccatcc gataggtagc tcaacttaat cccactcccc
     2941 acttgtgcga gattgtacaa aatatttttt tatggtagag ttacggagtg ccgcatttat
     3001 atcgctgcag gtagcaatac ttgaaatact tcaagctata attcgcttca aatcattgaa
     3061 ccagcggcaa cgtacatttt cactgtcctt tgagggtaga tgacgtcgta tgaatactga
     3121 caaaatccca gatatcataa ttggcagact gcctgtctat ttacgtgcac tccagcggtt
     3181 ggcagagaaa ggcatcagta ccacttcctc tcaagagctc ggtgaaattg tggggatttc
     3241 agctgcgcaa atccgcaaag atatttcgca attcggggaa ttcggcaaac aaggcacagg
     3301 ctacgccatt tcttatcttg ttgagaaatt gcaggatatt ttaaaagtgg accgcgtttg
     3361 ggaggtggtc attgttggca tgggcgacgt tggacacgcg ctcgctcatt atcaggggtt
     3421 cgctgaccgc ggctttcatg tgaaaatggt ttttgataat gacccgaaga agattgggga
     3481 ccagttcggt gaattccaga tctatgatac cgcctcgctc tctgagaaaa taaaacaaca
     3541 caaaattaag atcgccatga tcgccgtccc ggctagtgat gcacagacgg tggcagatca
     3601 gctcgtcaaa gcaggagtga aagcgatcct aaattatgcg ccgatcagtt tgaccgttcc
     3661 ggacggtgtg cgtgtgcagt atattgaccc ggtaacgcat ttgcagagga tgacctatta
     3721 tttgtagtcg gtggaacgta gtttgcaggg aatagtgtta tgaaaagcag gtctttccag
     3781 gttgcggaag cctgcttttt ttattagcca tccagcgggg atttgacggc cagtccgcct
     3841 ttttgtaaga cgtgggtata gatcatggtg gtttttacat ctttgtgacc cagcagttcc
     3901 tgcaccgtgc gaatgtcata tccgttctgc aggagatggg tagcaaacga atgccgaaag
     3961 gtgtgaggtg aaacaagctt ttggaccccg gcagatttgg cggcttgacg aatggctttc
     4021 tgcaggtagc tcgggtcagc atggtggcgc attgtctttt ttgagatcgg gtcggtggag
     4081 agggaaagag cagggaagat atattgccaa acccaatctc tggcggcacg ctggtatttg
     4141 cggttgaggg cataaggaag atacacttca ccaaagccgg ccttgaggtc tttttggtgg
     4201 atgtagccaa cgttttgaag gtgttgctgc aggtctggga tgagcgtctc ggggagggga
     4261 gtgaaacggt cttcttcgcc tttgccgtca cgcacgatga tctggcggtt gccgaagtca
     4321 atgtctttga cacgcaggcg cagacattca atgaggcgca acccgctgcc gtacagtatt
     4381 tttgtcataa tctgaggcac accgtgcagt ttatggatca ccgccatggc ctcggcgtgg
     4441 gtgagcacgg tggggagggt tttggacttt tcggcacgaa tgagttcggt tggaacctcc
     4501 atgggcttaa gcagaacatt tcgatagagg aagagtatgg cgctgagtgc ctggttttgg
     4561 gtggaagccg ccactttttt ttcggtggca aggttggaaa ggaaggcctg tatttcggca
     4621 gcgcccattt cggctggatg ccgcttttta tggaagagga tatagcgttt gatccattct
     4681 ttgtaggtct gttcggtgcg ataggagtaa tgtttgaggc gaatcgcatc agtgacctgg
     4741 tcgagaattt ttttgccggg gggttgaggg gttgtaactt tttgcatgtt gacctgctat
     4801 aaaggaaagg tttataattt acttatgcta ttttaaccca tatccgtcta aagtcccaaa
     4861 cgatttaggg gattaggcgg ctgaaggtcc gcccataagc tgttatacgg agtccgtcga
     4921 atcactagtt gggtgcctgt gataagggtt ataatcatac aattgatcca ccag
//
LOCUS       DASTJP010000632         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1108288, whole genome shotgun sequence.
ACCESSION   DASTJP010000632 DASTJP010000000
VERSION     DASTJP010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108288"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFQ23_18575"
     CDS             <1..504
                     /locus_tag="VFQ23_18575"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908660.1"
                     /translation="QAARLVVEAFREHWPDAWPTLEEALREVREMLESERICRIAVDE
                     QGNLLGIIGGIPGYDGNVWELHPLAVQPSRQGQGVGRALVEDLEAQVRFKGGLTVTLG
                     SDDEDNMTSLSGVDLYENLWEKIRDIRNLKKHPFAFYQKMGYVITGVVPDANGRGKPD
                     ILMAKRI"
     gene            complement(563..1600)
                     /locus_tag="VFQ23_18580"
     CDS             complement(563..1600)
                     /locus_tag="VFQ23_18580"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9908661.1"
                     /translation="MTLHSEIFEQPERLASLLENQKQLCFEIAKAIQSRNVQYAFLAA
                     RGTSDNAGRYANYLWGARNGLPLALATPSLFTYYQSPPRLSGALVVGVSQSGQSPDIV
                     SVLEEGRKQNCMTLAITNVPDSPLASAADFVLDIQAGPEKAVAATKTYTAELMAVAMI
                     SAALRNSEENWNELASVSKWAEQALALDHQIAQMAQRYRYMTQCVVLGRGFNYATAFE
                     WALKLKELTYLIAEPYSSADFQHGPIAMVEGGFPVLAVAPRGKVHDTMRDMLTRLRKE
                     KDAELVVISDDPDALVLAQSPIQLPPQIPEWLTPLVSIIPAQLFACHLTQVKGYDTDA
                     PRNITKVTETH"
BASE COUNT          611 a          519 c          542 g          539 t
ORIGIN      
        1 caagccgccc ggttagtggt ggaggcattc cgcgaacatt ggccggatgc ctggcccacg
       61 cttgaggagg ctctgcggga agttcgggaa atgctcgaaa gtgaacgaat ctgccgcatc
      121 gcagtggatg aacagggcaa tttgctcgga atcatcggcg gaatcccggg ttacgatgga
      181 aatgtgtggg aattacaccc gcttgccgtc cagccatcca gacagggaca aggagtcggc
      241 cgggcactgg ttgaggatct cgaggcgcaa gtccgcttca agggtgggct gacagttacg
      301 ctcggaagcg atgatgagga caacatgact tcgttatccg gtgtggatct gtacgaaaat
      361 ctatgggaaa agataaggga tatacgcaat ttaaagaagc acccctttgc gttttatcaa
      421 aagatgggat atgtcattac aggggttgtc cccgatgcga acggaagagg aaaacccgat
      481 atcttgatgg cgaaaagaat atgaggagtc aaagtctcca gactttggca attcaacgac
      541 tggagtgttt gactcctttc cattaatgcg tctctgtcac cttcgtaata ttgcgcgggg
      601 catccgtgtc gtatcctttg acctgggtca ggtgacaggc aaacaattgg gcggggatga
      661 tgctcaccag gggtgtcagc cactccggaa tctgaggcgg gagctggatc ggcgattgcg
      721 cgagcacgag cgcgtccgga tcgtctgaga tcacgaccag ctccgcgtcc ttctccttgc
      781 ggagacgggt caacatgtcc cgcatcgtat catggacttt cccccggggg gcaacagcca
      841 ggaccgggaa accgccttcc accattgcaa tggggccatg ctggaagtca gccgaggaat
      901 atggctcggc gatcagatac gtcagctctt ttagtttcag ggcccattca aaggcggttg
      961 cataattaaa tccgcgaccc agcactacgc attgagtcat ataccggtaa cgctgtgcca
     1021 tttgtgcgat ttgatgatcg agcgcaaggg cctgctctgc ccatttcgac acagaggcaa
     1081 gctcgttcca gttttcttcg gaattgcgca acgcggcaga gatcatggca acggccataa
     1141 gttctgcagt ataagttttt gtcgccgcga ctgctttttc agggccggct tgaatatcga
     1201 gcacaaaatc tgcggcgctg gcaagcggcg aatcggggac attggtgatc gcaagcgtca
     1261 tgcagttttg tttgcgcccc tcctccagca cactgacgat atccggcgat tgcccggatt
     1321 gtgaaacgcc aacaacaagc gcgccactca aacgcggcgg gctttgataa taggtaaaca
     1381 gcgacggcgt tgccagtgca agaggcaggc cgttacgcgc gccccaaaga taattcgcat
     1441 atcgcccggc attatccgat gtaccgcgcg ccgcgagaaa tgcatattgc acattacgcg
     1501 attgaatcgc tttggctatt tcaaaacaaa gctgcttttg attctcaagc agcgatgcga
     1561 gtcgttccgg ttgttcaaag atttcagaat gaagtgtcat attatcctct gcatgaattc
     1621 tacctcttgt cgcataaacc agcacctttt ttgaaagttg gttgcttgaa gttgccgtat
     1681 cacgttggat gaaggattca aaggagacac aggaagactt cttttcttcc cacagaagat
     1741 gaacaacagg caacacagga tcaaattcga ttcaaagcat gcgacaacat ctttcaacat
     1801 cgaaggcaca tcgatgtaca cataacaaca agatgatgaa tacgtttaca tcgttggtaa
     1861 atcaatacga attgatgatg aacatgattg aatctatcaa caaaaatttt ctttcattca
     1921 ttatcaaaat gaacactaac aaaattttaa acttattgtt catcaaaaac ctttacataa
     1981 actcattaat agatacaatc aacatagttg catataaaaa attatattga ttcaaattaa
     2041 atgattcaac gaatcatata acaacaaaat aatttatcaa cgatgcccat atgcaacaca
     2101 atcaagttca tgtcttctca acacatattg acttgattgt ttgaaataaa gaaaaggact
     2161 cgacatgtat ctctgtgtat tatcaagcat acctgatccc aatgatgttc c
//
LOCUS       DASTJP010000633         3673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_823633, whole genome shotgun sequence.
ACCESSION   DASTJP010000633 DASTJP010000000
VERSION     DASTJP010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823633"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /gene="ftsA"
                     /locus_tag="VFQ23_18585"
     CDS             <1..1209
                     /gene="ftsA"
                     /locus_tag="VFQ23_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET9908662.1"
                     /translation="DVGTTKVCTLVGRVEDNNSIRILGVGIEPSEGIRKGVIVDLSAA
                     SQTIMKSVKKAESTSGLEITSALVSIAGSHVSSVNSRGATAVNGNIIEQFDIDHALEQ
                     ARAVAIPHDREVIHVIQRGFTIDEQDGIRNPRGMHGYRLEVEAHIITAAAATVDNLRQ
                     CVGAAGVEIQQFVLNPLASGEVVLTDHERQMGVAVCDIGGGTTDLAIYVNGDVWHTMV
                     IAVGGNHITQDIAHGLRLPFAQAEEVKKQHGYAVRAEVGSEEYFSIRPVGEDRPVQIN
                     RQDLAHIIEARVSEIFSLTLQEIKRSGYDGLLPAGMVLTGGTSLLPGIKRVASDVLNM
                     PVRGAQPDNLTGLVDRLNSPAYSTGVGLLRWIFSMHDNDLSIGRGRRSKGEKRMDFGF
                     VKKLIGRILP"
     gene            1408..2589
                     /gene="ftsZ"
                     /locus_tag="VFQ23_18590"
     CDS             1408..2589
                     /gene="ftsZ"
                     /locus_tag="VFQ23_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET9908663.1"
                     /translation="MDSTKKQQPQDHWDKPGEAFARIKVIGVGGGGSNAVNRMIDEGI
                     QGVEFIVVNTDAQALLLAKAQTRVRLGEKLTRGLGAGGDPEIGRKAAEESADELYNVL
                     KGSDMVFVTAGMGGGTGTGAAPIVAQVAKESGALTIGVVTRPFTFEGMRRLQSADTGI
                     GKLKEHADTLIAIPNDRLLQVADKRASLQDAFRLADDVLHQGIQGISELITIPGLINL
                     DFADVRAIMSEGGAALMAVGTGSGDDRAKKAAEAAISSQLLDITIDGARGVLFNVTGG
                     PNMTLFEVNQAAAIIRETAHPDVNMIFGAVIDPNMGDDIRITVIATGFDRSSMPRRAL
                     ERQPRLDVRRSESAMYMRPTESVSVHADSQSGETKHTGSSLSSAAKDDLDIPTFLRNR
                     R"
     gene            2792..3211
                     /locus_tag="VFQ23_18595"
     CDS             2792..3211
                     /locus_tag="VFQ23_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908664.1"
                     /translation="MKAISPTPVHESYKQHRKQLISQIILPVVISALFIVGLIVLVNV
                     ATFRDNGDVGRWAAISTIWIVIPVMVAGLIFFILLGGIVYLLKYLLDITPGYTRQAQE
                     FVHLILSYIERAADAAVKPVFFIDGLGASIERFLGRK"
     gene            3214..3408
                     /locus_tag="VFQ23_18600"
     CDS             3214..3408
                     /locus_tag="VFQ23_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908665.1"
                     /translation="MNQNRILLFGALIGAFTGLIAAMILQRRADRTGTEISLSAGEGV
                     QLGVMVMGLLRAIAALGDDK"
BASE COUNT          879 a          917 c         1000 g          877 t
ORIGIN      
        1 gacgtcggca caaccaaagt ctgtacgctc gtaggacgag tggaagataa caactctatt
       61 cgcattcttg gtgttggcat tgagccttcc gagggaattc gtaaaggcgt gatcgtggat
      121 ctatccgctg cttcacagac gatcatgaag tccgtaaaaa aggccgaatc aacgtctggc
      181 ctggagatta cctcggcact ggtcagtatt gcgggctcgc atgtttcctc ggtgaacagt
      241 cggggtgcga ccgccgtgaa tggaaatatc atcgagcaat ttgatattga tcacgccctg
      301 gaacaagcgc gcgcagttgc aattccgcat gaccgcgagg tgattcacgt catccagcgc
      361 ggctttacga tcgatgaaca ggatggtatt cgcaacccca gaggcatgca tgggtatcgg
      421 ctggaagtgg aggcgcatat cattaccgcg gccgctgcaa cagtggataa cctgcgtcag
      481 tgtgtgggcg ctgcgggggt agagattcaa cagtttgttt taaatccact tgcgtctggt
      541 gaggtcgtgc tgaccgatca tgaacgtcag atgggtgttg cagtctgtga tatcggtggc
      601 ggcaccacag atctggcgat ctatgtcaat ggtgatgtgt ggcacaccat ggtcattgca
      661 gttggcggaa atcatattac acaggatatt gcacatgggt tgcgcttgcc gtttgcgcag
      721 gcggaggagg tgaaaaaaca acatggctac gcagtgcggg ctgaagttgg ttcagaggaa
      781 tatttttcga tccggcctgt gggtgaggat cgacccgtgc aaatcaatcg acaggacctg
      841 gcgcatatca ttgaagcacg cgtcagtgag atcttctcac tgacattaca ggaaatcaag
      901 cggtctggct atgatgggct actgccggct ggcatggtgt tgacaggcgg tacatcctta
      961 ttgcctggta tcaagcgcgt tgcatccgat gtgttgaaca tgccagtgcg cggcgcgcag
     1021 ccggataacc tgaccggtct tgtggatcga ttgaactcac cggcctattc aacaggggtg
     1081 ggacttttgc gatggatatt ttcaatgcac gacaatgatt tgagcatcgg acgtgggcgt
     1141 cgctcgaaag gagaaaagcg tatggatttt ggttttgtca agaaattaat cggccggata
     1201 ttgccataac aaattcttcg tcctgagcgg agcacagtgc agacgacact tgcacctgcg
     1261 tgcacaccgc cccggttggt cggtgccggg gagtgcaggt gaggacaggt ttgcaaatgc
     1321 gcttcgacta cgctactctc cgctcagcgc gaagatataa acctaaaaag aaagaatcta
     1381 ttcataatct ttgatggagg taccaaaatg gactcgacca aaaagcaaca accgcaagac
     1441 cactgggaca aaccaggaga agccttcgcc cgcattaaag tgatcggtgt aggtggcggt
     1501 ggatcgaacg ccgtcaaccg catgatcgat gagggtattc agggcgtgga gttcatcgtt
     1561 gtgaacaccg acgcccaggc attgcttctg gcaaaagcac agacccgcgt gagactcggt
     1621 gaaaagctca cccgcggtct cggtgccggt ggcgaccctg agatcggccg caaagctgca
     1681 gaagaatcgg cagacgaact ctataacgtg ctaaaaggct cggatatggt cttcgtcacg
     1741 gctggcatgg gcggtggaac cggcaccggc gcagcaccga tcgtggctca ggtcgcaaag
     1801 gaaagcggcg cgttgaccat cggcgttgtg acgcgtccct tcacgtttga aggaatgcgc
     1861 cgtttgcaat ccgctgatac aggcatcggc aaactcaagg aacatgccga taccctgatc
     1921 gcgattccga acgaccgctt attgcaggtt gcagataagc gtgcatccct gcaggatgcc
     1981 ttccgccttg ccgatgatgt gttgcatcag ggtattcagg gcatttcaga actgatcact
     2041 atccctggtc tcatcaacct ggatttcgca gacgtacgcg ccatcatgtc tgaaggtggg
     2101 gcagcgctta tggcagtcgg aacaggttcc ggcgatgacc gagcgaagaa ggcggctgag
     2161 gctgcaatct ccagtcaact gctcgatatc acgattgatg gtgcacgtgg cgtgctcttc
     2221 aacgtcaccg gcggcccgaa catgactctc ttcgaggtga accaggctgc agccatcatc
     2281 cgcgagacag ctcacccgga tgtgaacatg atcttcggtg ccgtgatcga cccgaacatg
     2341 ggcgacgaca ttcgaatcac ggtcatcgcc accggcttcg accgcagcag catgccgcgc
     2401 cgtgccctgg aacgccagcc gcgcctggat gttcgacgat ctgagtctgc catgtacatg
     2461 cgacccactg agtcagttag cgttcacgcg gattctcagt ctggtgagac gaaacacaca
     2521 ggctcgtctt tgtcctcggc tgctaaagat gacctcgaca ttccgacctt tttgaggaat
     2581 cgtcgataat accatgtagg gatgggcagc tgcccatccc tacatggtga aaaaatatat
     2641 attaggttaa gtccatttcc catttcctgg ggcggtcttg acgtgcctgg gcgaggtgcg
     2701 tcgagccgcc ctgcgcgtct actaccctac cctaatcact acggaatttc ccccaaatcc
     2761 tatctgaatt aattatgcta caatcgagac catgaaagcg atcagcccta caccggtcca
     2821 cgaatcttat aaacaacatc gtaaacaatt aatctcgcag attattctgc ctgttgtcat
     2881 ctctgcactt ttcatcgttg gattaatcgt gttggtcaat gtggctacgt tccgcgataa
     2941 cggcgatgtc ggccgctggg cagccatctc aaccatctgg attgtcattc ctgtaatggt
     3001 cgccgggctg atttttttta tcctgctggg cggcattgtt tatctattga aatatctact
     3061 tgatatcact cccggctata ccaggcaggc acaggaattc gtccacctga tcttaagtta
     3121 tatagaacgc gctgcagatg ccgcggtcaa accggtgttt tttattgacg gactgggcgc
     3181 tagtatcgaa cgatttttag gaaggaaatg attatgaatc aaaaccgaat tttgctgttt
     3241 ggtgccctga ttggcgcttt tactggtctg atcgcagcga tgatccttca gcgtcgcgca
     3301 gaccgaaccg gaacagaaat ttccctttct gcgggtgaag gcgtccaact cggtgtgatg
     3361 gtcatgggac ttttacgtgc cattgcagca ctgggtgatg ataagtagac cctaagtttt
     3421 gcattgccct gtaaaataga gacacgatat actgagtagg tcttatcgtg tctctattgc
     3481 ctttaataac gtagtatcaa ctttccaaat atcccaacca agcctgcaga acatggtcga
     3541 ctctaagaat acagtaattt atggcaagga tcatgaatct ccgaacgaga tccattgcca
     3601 gccaattgaa agtctaaata ttgctctggg aatcagcgaa ggtgtcacgt caaaacccaa
     3661 cgaggattgc ctc
//
LOCUS       DASTJP010000634         7329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_725094, whole genome shotgun sequence.
ACCESSION   DASTJP010000634 DASTJP010000000
VERSION     DASTJP010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725094"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFQ23_18605"
     CDS             <1..215
                     /locus_tag="VFQ23_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908666.1"
                     /translation="LLDMHLPHVSGAELLTNLIKVDERFAKTMVIITTADARMGDTYG
                     DQVDFVLIKPISFVQLRDLTARLKPK"
     gene            complement(222..1847)
                     /locus_tag="VFQ23_18610"
     CDS             complement(222..1847)
                     /locus_tag="VFQ23_18610"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HET9908667.1"
                     /translation="MKTNPIRFWSIVILLGWAFDFLFWKKPGGVNFAIYVALGIVTGI
                     FLLRLDGLRLSPRSGLLLIPIAFLAPMTFLRLEPLTVFLSVAMTLFLIGVFAITFLGG
                     RWLNYALLDYVFGYFRLFASMIARPLGFVAEARRVPAVQLDGEESNGSAQRRTILLPI
                     LRGIVIALPIIAIFASLLSSADPIFASRFEDLIALFRIENLPEYIFRLVYILVFAYAL
                     AGAYLHAAHSSDEKISEDKPLVPPFLGFTEAGIVLGSLAILFVAFVAIQFQYFFGGQA
                     NISIEGYTFSEYARKGFGELVTVAIFSLLVILGLGAITRRDVESQRRVFSGLSVGIVG
                     LVIVMLVSAFQRLVLYEEAYGFSRLRTYTHVFMIWLGILLIAVVILEILRRERRIGLT
                     IILASLGFVISLNVLNVDAFIVKQNVQRELRSLADPLLSQGREDLDVQYFLDLSDDAV
                     PVLAAAFENKSLPEVVNEKLGAALACKRHERGLDQRALPWQSFHFSRYYADRAFIELD
                     KELDAYKITDTDWPVTVETPGGDEFSCWQYYYD"
     gene            complement(2242..3324)
                     /locus_tag="VFQ23_18615"
     CDS             complement(2242..3324)
                     /locus_tag="VFQ23_18615"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET9908668.1"
                     /translation="MKRLGILTGGGDAPGLNAVIRAAVKTAIYEYDCEVFGIRNGYDG
                     FIDEKGVVPLGINEVRGLLLRGGTILGAANRGNPYARKVIRDGQEVTIDVSDAIVKGM
                     DKLKLDALLILGGDGTLRSAHELHLKGVPVIGVPKTIDNDIGGTEVTFGFDTAVTTAT
                     EAIDKLSTTAEAHHRVMVLELMGRNAGFIALHAGVAGGADVILIPEIPFKYETVVAKI
                     RERVERGYLFSIVAVSEGANALGGAQVFSRTGNEFYTPRLGGICHTVGEYVENQGYET
                     RVTVLGHVQRGGTPTPFDRWLATRYGAAAVRLAAQGRFDRMVSLQAGKIVDVSLEEAI
                     SIPKRVDVNDDSVITARGMSISFGDE"
     gene            complement(3436..3729)
                     /locus_tag="VFQ23_18620"
     CDS             complement(3436..3729)
                     /locus_tag="VFQ23_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908669.1"
                     /translation="MIPHKQGKRQSPLLDNKSSSLVRASSTGKVGSIVGVAGTGGSVG
                     NGLGEAVGNGVNEGAGVALLAGREGVSEDRIVMLSVDCAQLESSTTKSIKLKQ"
     gene            3803..4414
                     /locus_tag="VFQ23_18625"
     CDS             3803..4414
                     /locus_tag="VFQ23_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908670.1"
                     /translation="MNPPEPGSITPRYTEGDLEIYNRVAFITNLDSSIINSIAFTVEA
                     HRILKEGGYEEVFDSKFFGDKVSTYMLAHVLSEHGVPSSVMISTLGGPLTRGGTGGFD
                     ILLLYPDQGILFNYTTQMQVVGTNVRGCLSNAHVEMELYPPGQADSFFESLQQTDWAV
                     KMGYYKPLEEATSMSIEEFYETFREPGNQCIETPANLWPTPEP"
     gene            complement(4559..5383)
                     /locus_tag="VFQ23_18630"
     CDS             complement(4559..5383)
                     /locus_tag="VFQ23_18630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9908671.1"
                     /translation="MEAKGKVVIVTGASSGIGEATAREFAKEGSKVVLAARRVDRLEA
                     LAKEIESMNTGAQALVVQADLSKLEDIQSLITKTLERFGRIDILVNNAGFGRLDWLEN
                     LDPVKDIQSQFDVNVMGVVQTTRQALPVMIKQRSGHVINMCSMAGLVATPTYTIYAAC
                     KHAVHGFSEALRREVKPWGIDVSMIYPGGVTTEFGAHAGIKRKTNATTPRFMLLTAEQ
                     VGQEVVNLVRNPRAMRIIPWLWTFTVWMNRNFNSFVDYTTINRFTIPEREEELKVR"
     gene            5613..6449
                     /locus_tag="VFQ23_18635"
     CDS             5613..6449
                     /locus_tag="VFQ23_18635"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HET9908672.1"
                     /translation="MENVSSQTRINNITRWFVPAAALLVFAVWFYITPPGLLGKADAI
                     GYAICHRIDERSFQIHDRQLPLCARCTGEFYAAGISLLFFALVSPRKSGMPGWKLGAP
                     LLFFLVAFGLDGSNSYLYLIKQTAAGALDHIPNLYTPNNTLRLLTGSGMGIALASIVY
                     PAFNQTVWRHSDPERALDWKKLGILISIILLIDLIILTEQPDILYIIAILSVLGVLAL
                     LVIVFSMSWLLIMRQDNSFDSLRQMWMPFLAGTTLAFLMITVIDLLRFQLTGTWGSFP
                     LG"
     gene            6586..7152
                     /locus_tag="VFQ23_18640"
     CDS             6586..7152
                     /locus_tag="VFQ23_18640"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HET9908673.1"
                     /translation="MDLLSGIFTAFGLSASAGLNAYIPLLLVGLIARYTELMKLSAPW
                     DTLSHPGIILMLCVLVIIEMLADKVPVVNHINDLIQTLIRPAAGAVAFAASSNVVTDV
                     SPVLALACGLLVAGTVHVAKAGAVRPIVTATTGGTGNVPVSIAEDIISTVLSILAIVL
                     PVLIGTLMVVLAAFIIWWLYRRANRQTT"
BASE COUNT         1846 a         1994 c         1727 g         1762 t
ORIGIN      
        1 tccttttgga catgcattta ccacacgttt caggtgccga gctattgacg aacctcatca
       61 aggtggatga gcgcttcgca aaaacgatgg tcatcatcac aacggcggac gcgcgcatgg
      121 gcgacaccta tggggatcag gtagatttcg ttttgatcaa accgatctcg tttgtacaat
      181 tgcgtgacct cactgcccga ttgaaaccaa aataacaacg attaatcgta ataatactgc
      241 cagcacgaga attcatctcc gcctggtgtc tcgaccgtta cgggccaatc ggtgtccgta
      301 attttgtatg cgtccaactc cttatctaat tcaataaagg ctcggtcggc gtaataacgc
      361 gagaagtgaa aggattgcca cggaagggcg cgctgatcaa gtccacgctc atgacgctta
      421 caagccaatg ccgctcccaa tttttcattg accacctctg gcaaggactt attctcgaat
      481 gcggcagcca gcactggaac tgcatcgtcg gaaagatcaa gaaaatattg cacgtccaga
      541 tcttcacgcc cctgtgaaag tagagggtca gccagactgc gcagctcgcg ctgaacattt
      601 tgcttcacaa tgaaggcatc cacgttcaag acattgaggg aaatcacaaa tcctagagaa
      661 gccagtatta ttgtcaagcc gatcctgcgt tcgcggcgca ggatctcaag gatcacaacg
      721 gcgataagca gaatcccaag ccaaatcatg aaaacatggg tataggtacg taagcgcgag
      781 aacccatacg cttcttcgta caacactaag cgctggaacg ccgagaccag cattacgatc
      841 accagcccaa caatgcctac actcaaacct gaaaagacac gccgctgcga ttcaacgtcg
      901 cggcgcgtga tagctccaag ccccagaatt accaacaggc tgaagatagc aactgtgacc
      961 agctcgccaa aacccttgcg tgcatactca gaaaaggtat aaccttcaat gctaatgttg
     1021 gcttgccctc caaagaaata ctggaattga atcgctacaa aagcgacgaa caaaatagcc
     1081 aaactgccaa ggacgattcc ggcctccgta aaaccaagaa agggcggaac cagcggtttg
     1141 tcttctgaaa ttttttcatc ggaactgtgg gccgcatgta gatacgcgcc cgccagggcg
     1201 tacgcaaaga caaggatata gacgaggcgg aaaatatact caggcaaatt ttcaattcga
     1261 aataatgcaa tcaggtcttc aaaccgactc gcaaagatag ggtccgctga tgaaagcagg
     1321 gaggcgaaga ttgcgatgat tggcaaggcg atcacaatac cgcgcaaaat gggcaacagg
     1381 attgtacggc gttgtgcact tccattggat tcttcgccat ccagctgcac cgcaggaacg
     1441 cgacgcgcct cagcgacaaa ccctaatggg cgagcgatca tactggcaaa taaccgaaaa
     1501 taaccgaaga catagtctaa cagcgcataa ttcagccagc gaccgccgag aaacgtgatg
     1561 gcaaagacgc ctatcagaaa gagggtcatg gcaacagaaa ggaaaaccgt caacggctcc
     1621 aggcggagaa acgtcatggg cgccagaaac gctatcggga tcaggaggag gcccgagcgc
     1681 ggcgagaggc gaagcccatc cagccgaagg agaaaaatac ccgtaacaat accgagggct
     1741 acataaatcg cgaagttgac tccccctggc ttcttccaaa aaagaaagtc gaacgcccag
     1801 cccagaagaa ttacaattga ccaaaagcga ataggatttg ttttcatgcg tgcctccacg
     1861 aaaaaatttc ccccagagta gcgctgaggg gctcgcggag actctcaaaa ggtatcacat
     1921 tttcacaagc tgcctgtaca gaaggattac aaatcgataa gaatcaattt tgggctattg
     1981 acaggtccat tgaaatatga catacttcta tcattattcg ggggcaaaca aattgagccg
     2041 ccatcctata gaaaggagga ggtgatgtct aacatggata gtagtatcaa acccagcgct
     2101 gtggcatccc tcctcaatta agaaggaagc cacagcgccc accaatgcca cccgaaaggg
     2161 tggcattgtg gtttaagccc caccccaacc ctccccaaat tctgcgaatt tggggaggat
     2221 gccgcccagc ggcgggaggg gtcattcgtc gccaaacgaa atgctcatcc cgcgggcagt
     2281 gatgaccgag tcatcattca cgtccacgcg tttggggatt gagatcgctt cttccagcga
     2341 gacatcgacg atcttcccag cctgcaagga gaccattcgg tcaaacctac cctgtgctgc
     2401 caaacggacg gctgctgcac cgtagcgggt cgccagccag cgatcaaagg gagtcggggt
     2461 tcctccacgc tgaacgtgac caagcaccgt tacgcgggtc tcatatcctt gattttcgac
     2521 gtattccccc accgtatgac aaatcccgcc aaggcgcggg gtataaaatt cgtttcctgt
     2581 acgcgagaat acctgggctc cgccaagtgc attggctccc tctgagacgg ctacaatgct
     2641 gaagaggtac ccgcgttcca ctctctcacg gatctttgcc acgaccgtct cgtacttgaa
     2701 cgggatctcg ggaatcagaa tgacatccgc gccgcccgct acccccgcat gcagcgcaat
     2761 gaagccagcg ttgcgcccca tcagctcaag aaccatgacg cgatgatgcg cctcggcagt
     2821 cgtgctcaat ttatcaatgg cctccgtggc ggtcgtcacc gccgtatcaa atccgaaggt
     2881 cacttctgtt ccgccgatat cattatcaat cgtttttggg acgccaataa caggcacacc
     2941 tttgagatgc agctcatgcg cactacgcag ggttccatca ccgcctaaga ttagcagcgc
     3001 atccagcttc aatttgtcca tgcctttgac aattgcatcc gagacatcga tcgtgacctc
     3061 ttgcccatca cggatgacct tgcgcgcgta cgggttccct cgatttgccg cacccaggat
     3121 cgtcccacct cggagcaata agccacgcac ctcattgatg cccagtggaa caactccttt
     3181 ttcatcaatg aacccatcat agccattgcg gatgccgaag acctcacagt catattcata
     3241 gatcgcggtc ttgaccgccg cgcgaatgac cgcatttagc ccaggcgcat ctccgccacc
     3301 tgtaagaatt cctagtcgtt tcatgaatgt tctcctctga tgtcaatgat accgcagagg
     3361 tgaatttttt ctgtattcaa tcgttcgtag cgtacaatta aaataagagg aacacatgaa
     3421 gccacacaat tttgttcact gtttaagttt gatacttttc gtggtactgc tttccagttg
     3481 tgcgcagtca actgaaagca taacaatcct gtcttcagaa acgccttcgc ggcctgccag
     3541 tagtgccaca cctgcacctt cattgacgcc gttacccaca gcctcaccta aaccattgcc
     3601 aacggaccca cctgtgccag ccactccaac gatgcttcca accttgcctg tagaggaggc
     3661 acggacaaga ctactggatt tgttatccag taatggcgac tgccgtttac cctgtttgtg
     3721 gggtatcaca cccggggaga gttcctttta cggtgctcgg gaaatattag cgccgctcag
     3781 tagcttatca cattctgtat acttgaatcc tcctgaaccg ggtagtatta cacctaggta
     3841 tactgaaggc gacttagaaa tctataatcg ggtagccttc attaccaatc tggacagtag
     3901 catcataaat agtatcgcct ttactgtaga agcccacaga attctaaaag aaggcggcta
     3961 tgaagaagta tttgactcaa aattctttgg cgataaggtc agtacctaca tgctcgcaca
     4021 tgttctatcc gagcatggtg ttccatcatc cgtcatgata tccacgcttg gcggaccgct
     4081 gactcgtggt ggcactggcg ggtttgatat tctattgcta tacccagatc aaggcatctt
     4141 atttaattac acgacccaaa tgcaagtggt tggaacaaat gtacgtggat gcctttcaaa
     4201 cgcacatgtt gagatggagt tatatcctcc cggacaagcc gattcgtttt ttgaaagttt
     4261 acaacaaacc gattgggcag taaagatggg ttattacaaa cctctcgaag aagcgacatc
     4321 tatgtcaatc gaagagttct atgaaacatt tcgtgaaccg ggtaatcagt gtatcgaaac
     4381 accagcaaat ctctggccca cgccagaacc gtaattgcta ttccagagat ctacctttac
     4441 tttcagcgga aaacgcatgg ggaagtgcaa tgtacataat gattttttta cactgccaag
     4501 cgataaactt ggtatgatat aaaatatcag ggctgttctt cacgaacagc cctgatctct
     4561 atcttacttt caactcctct tccctttcgg gaatggtaaa acgattgatc gttgtgtaat
     4621 ccacaaagct attgaagttt cggttcatcc agacagtgaa tgtccataac cacgggatga
     4681 tccgcatggc gcgcgggttg cgtaccaggt tcacaacttc ctgcccaacc tgttccgccg
     4741 tgagcagcat aaatctcggt gtagtggcgt tcgttttgcg cttgatgccg gcgtgcgcgc
     4801 caaattcggt ggtcacaccg ccggggtaga tcatcgaaac gtcgataccc cagggcttga
     4861 cctcacgtcg cagcgcttca gagaatccgt gcacggcgtg tttgcaagcc gcgtaaatag
     4921 tgtaggtcgg cgtggcgacc aatcccgcca tcgagcacat attgataaca tgacctgacc
     4981 gctgcttgat catgaccggt aaggcctgac gcgtcgtctg aacaacaccc attacattga
     5041 catcgaactg cgactggata tctttcaccg ggtcgagatt ctcaagccag tcgagccggc
     5101 caaaacctgc gttgttgacc agtatgtcaa tgcgaccaaa tctttcaagg gtttttgtaa
     5161 tgagagattg aatatcttcg agtttggaga gatcagcctg cactaccagt gcctgtgccc
     5221 cagtgttcat ggactcgatt tctttcgcga gtgcttccaa acggtctacc cgacgtgcag
     5281 caaggaccac ctttgacccc tcttttgcaa attccctggc ggttgcttca ccgattcccg
     5341 atgaggcacc cgtgacaatg acaactttac cttttgcttc catttggtcc tcctggattt
     5401 aataagatca tgtttggaaa accagaaacg tatgtgtggt gcaatattac cacgcgtcaa
     5461 tattacggat gtccatgtga ggagtggtcg ctgcggcgaa gcaatcttct gaaaattatg
     5521 agattgcttc gtcagcccta tggggctttc tcgcaatgac gtaggtaaaa cctttcttgc
     5581 acgcagtcgt atatacccat ataatgagcg caatggaaaa cgtctcttcc caaacccgaa
     5641 tcaacaatat aacacgctgg tttgtccctg ccgcggccct tctcgtcttt gcagtctggt
     5701 tttatatcac tccgccgggt ctgctcggca aagcggatgc gatcgggtat gcgatttgcc
     5761 atcgaattga cgaacgttcg tttcagatcc acgaccgcca gcttccgtta tgtgcgcgct
     5821 gtaccggtga attctacgcc gctgggatct ctctgctctt tttcgcgcta gtcagcccca
     5881 ggaaaagcgg catgcccggt tggaagctcg gcgctcccct tctgttcttc ctggttgcct
     5941 tcggacttga cggctcaaac tcttatctct atctgatcaa acaaaccgca gccggggctc
     6001 tcgatcacat cccaaacctt tacactccaa acaacacatt acggcttctt accgggagtg
     6061 gaatgggtat tgccctggct tcgatcgttt acccggcgtt taaccaaacc gtctggagac
     6121 acagcgatcc ggagcgggcg cttgattgga aaaagcttgg tattctgata agcatcattc
     6181 tgctgatcga cctcattatt ctcactgaac agccagatat tctttacatc atcgccattc
     6241 tcagcgtgct gggtgtcctg gccttgctcg tcattgtatt cagcatgtcc tggttgttga
     6301 taatgaggca ggataattcc ttcgactcgt tacgacaaat gtggatgccc ttcctcgctg
     6361 gtaccactct cgcttttttg atgatcactg tcattgactt acttcgcttc caactcaccg
     6421 gaacctgggg ctcgtttccc ctgggttgag tatttccttt cgcgctgagc ggagggtaga
     6481 tttcttccta cccacaaacg aagcgcactt tttagaacag cgcccttcgc ccactctcct
     6541 catagaggct cgtcgttccg ctcagggcca agaaatggag taactatgga tcttcttagt
     6601 ggaattttca ccgccttcgg actctccgcc agcgccggct tgaacgctta tatcccgctg
     6661 ctgctggttg gtctgattgc gcgctacacc gagctgatga aactcagcgc gccatgggat
     6721 acgctttccc acccggggat catcctcatg ctgtgcgtac tggtcatcat tgaaatgctc
     6781 gcggataaag tacctgtcgt caatcacatc aatgacctaa tccagaccct cattcggccg
     6841 gcggccggag ccgttgcctt cgctgccagt tcgaacgtcg ttacagacgt cagtcccgtg
     6901 ctggcgcttg cctgcggtct gttggtggca gggaccgtac acgttgccaa agctggggcg
     6961 gtgcgtccga tcgtcacggc aaccacgggc ggcacgggaa atgttcccgt atccatcgcc
     7021 gaagacatta tttccacggt gctatcgatt cttgcgatcg ttctgcctgt tctcatcggt
     7081 acgttgatgg tggttctggc cgcctttatc atctggtggc tgtaccggcg agccaaccgg
     7141 caaactacct gatataatcc agcgcatcat ttcaacacag gagtaatcat tatgaacgat
     7201 caattgcccc ccaccatttc cgacgaaccc aggagaaata acacgcccat tatcattggg
     7261 gtagctgcag tattgttgtg ttgttgttgt gccacattgt tattaggctg gttctacggc
     7321 gaccagatc
//
LOCUS       DASTJP010000635         6849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1108446, whole genome shotgun sequence.
ACCESSION   DASTJP010000635 DASTJP010000000
VERSION     DASTJP010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108446"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFQ23_18645"
     CDS             <1..310
                     /locus_tag="VFQ23_18645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9908674.1"
                     /translation="LVKSAEKAIRKSDIIARLGGDEFAVLFPETNQEVAPILLARIFD
                     SFKTAINAKGWPITFSVGVLTCRVAPPSIHELIDIADKLMYSAKTEGKNSTKYLIYSG
                     "
     gene            493..1176
                     /locus_tag="VFQ23_18650"
     CDS             493..1176
                     /locus_tag="VFQ23_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908675.1"
                     /translation="MMLPDYWLTRPGANPDESTQKAFDELWDSTLRIGGCPTIPFTLP
                     STKWQFLCHLADHHDIAVHGSGDPDITLFEPRQSNDLHEFGNQKAVYAASDGLWAMFF
                     AVVDRDRVNSITNACVRLEDETDTVHGPYYVFSISQSALPNQPWRTGTVYLLPHSTFV
                     TQPSIAFGAYQAHIAQLASFEPVQPLAKLTVTPEDFPFLAQIRGHDDERLQEYATALQ
                     TGAPWPKDS"
     gene            1216..1848
                     /locus_tag="VFQ23_18655"
     CDS             1216..1848
                     /locus_tag="VFQ23_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908676.1"
                     /translation="MENYENKLLSLQVQVMEPPKTSIPYSKLLALVSITESALLEKAL
                     KELAQDLPFKTNSWPSFGGYVEDWYMVELSEFKRNLLYCERGGYDLRHKCANGIDLVF
                     EILRIILPGYTETARSVAAGLDRIADQRLLLWLQMSSEIYHMYQVNEYFGLRVATYNV
                     TWMCQELVRMGVFANESEAFESEEHILKGLMSSGMPDEFARRLLHQLKHA"
     gene            2134..3387
                     /locus_tag="VFQ23_18660"
     CDS             2134..3387
                     /locus_tag="VFQ23_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908677.1"
                     /translation="MASTNTDARNSPTETQSAGERRVHIIGAGPVGLLLAALLQPIEG
                     LAVHLYEKRHEYTRTRMVQLSSYLVADSVESYRTDHIDGDTIIAVFDPPEIDEGLAFR
                     RAIPADLMTLLKQWTLGFVPLNTIERSISDLIDARKTNTMHRTAAVVTAEEAITMLRP
                     GDILIDCTGTKSLLRDDLIPDSGEVPGANTLTIRLEYALVVAFLYGQKYDCNEVCKYA
                     KNIENPQYKFIPMVQRTHYDGSVTHVTGIVNITAEEYEAMPSRFDGQWLRNNFPAPAQ
                     SMDRFIDKIKEETHGEILGDLEIIRIPLNLYRARNATNRQWLKTEHVNHPFASSPVFL
                     AGDSAIGSPYFQSISLGFECAMFLAGLIAQPDLPLKEMLDRYELHTYKQWLRVYMRSK
                     MIKHNKNLFESIDNKDALLDQLHIY"
     gene            3435..3752
                     /locus_tag="VFQ23_18665"
     CDS             3435..3752
                     /locus_tag="VFQ23_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908678.1"
                     /translation="MLNLPQPGQRLKITNSDYIVISEEWVVLTVEGTIITFSCKKWWD
                     YNSITMQGQTSETVIGEIAKADDKEVIIRFTQYSSIAVEVGLAIHGDQATYLKRLDPP
                     AYA"
     gene            3879..4271
                     /locus_tag="VFQ23_18670"
     CDS             3879..4271
                     /locus_tag="VFQ23_18670"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9908679.1"
                     /translation="MDNDNGLDVEYYTSELLKGLPFSAAVRVGKMLYLSGQIGIDHSG
                     KLVAGGISAETRQTMENIRATLEEYGSSLDHVVKATIMLAEMSEWSEMNKVYITFFSQ
                     HLPARSALGANGLALGARVEIECIAVLR"
     gene            4291..4788
                     /locus_tag="VFQ23_18675"
     CDS             4291..4788
                     /locus_tag="VFQ23_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908680.1"
                     /translation="MNNQNISIPLKRFLLIEGCPEDWRGLDLYLFRDNDVVFYVGQSH
                     LAFARVWKHLLSGFKGHSIVGRFVWCNWPRSMSFTIELFSSQSEQFGEVGNELNASER
                     LLIQRWSPCFNVSQNSQPTPVPAAYLPPNAPFRRRRSLLALTHEAARAVKAEDAKLWA
                     QNVEW"
     gene            4883..5167
                     /locus_tag="VFQ23_18680"
     CDS             4883..5167
                     /locus_tag="VFQ23_18680"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HET9908681.1"
                     /translation="MIFEFTSKIIYWRGPAPWFFVTMPAKQSRELKAVSSLVTYGWGV
                     IPVHVRIGKTEFTTSLFPKDGRYLVPIKASVRKAENIEKGDKVTVRLEIH"
     gene            5317..5697
                     /locus_tag="VFQ23_18685"
     CDS             5317..5697
                     /locus_tag="VFQ23_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908682.1"
                     /translation="MKAVQFFLFVILSTIFLSACAATSEPAATPFPESMKGYELYSWQ
                     DGSQWKFSILVGTNREKSLDEIKTADVAISGIEELKSTLEQIPEGQYITWSTRETLSF
                     PPEEILKQVEQTCSDKGLILNIAL"
     gene            5705..6400
                     /locus_tag="VFQ23_18690"
     CDS             5705..6400
                     /locus_tag="VFQ23_18690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9908683.1"
                     /translation="MQTGRDNLEEFDDPPNYDIEEAERSAARIAYYCELARSIGGPVL
                     EIACGSGLVAIPVAAQGLNVTGVDLSRPMLEHARKKADEQHLNIRWVEADARSFDLGT
                     QYQFIYLTGNAFQAFLSREDQETLLASVKRHLAPDGIFAFETRNPSGHDLSDQPEPEF
                     DQSYVSVEGHQVSVYVSQRYEPLTQVMYWTTFRHWNDGERDHTKQTHIACRFTHPQEL
                     EMLLHYNGFQIIQ"
BASE COUNT         1794 a         1724 c         1683 g         1648 t
ORIGIN      
        1 attagtaaaa tcagcagaaa aagctattcg aaaatccgat attattgcgc gtcttggagg
       61 agatgagttt gcagtgctat ttcctgaaac aaatcaggaa gttgctccaa ttttattggc
      121 tagaattttc gactctttca agacagcaat caatgcaaag ggttggccca taaccttcag
      181 cgtaggtgtt ttgacatgcc gggttgcgcc accgtcaatc catgaactaa tcgatatcgc
      241 cgacaaattg atgtattcag caaagacaga aggtaaaaat tcaactaaat atctaattta
      301 ttcgggctaa caccatccca atcgggatgg gcatccccag cactgccacg cgccgttcaa
      361 gatttgcgat acataggtga ttgcagcgga atgctccttg gcgcctctcc ctatcctgct
      421 cggaaagagc agtcccagca gtgctacgcc gacgtaagac ctgaggcagt tattgagaat
      481 ggagtcatat ttatgatgct accggattat tggttaacac gacctggcgc aaacccggac
      541 gaatcaactc aaaaggcgtt tgatgaattg tgggattcaa ctcttcgtat cggaggttgc
      601 cccaccattc cgtttacttt gccatccacg aagtggcagt tcctctgcca tctcgcagat
      661 catcacgata tcgccgtgca cggttctggc gacccggaca tcactctttt cgaaccacgc
      721 cagtcgaatg acctccacga gtttggcaac caaaaggccg tctatgcggc atccgatggt
      781 ttgtgggcca tgttttttgc cgtcgtagat cgggaccgag tcaattcgat caccaacgcc
      841 tgtgttcggc tggaagatga aaccgatacg gttcatggac cgtattatgt gttctccatc
      901 agtcagtctg cgcttccgaa tcagccctgg cgtacgggca cggtctatct tttgccacac
      961 agcacattcg tgactcaacc atccatcgcc tttggcgctt accaggccca tatcgcgcag
     1021 cttgcaagtt ttgaaccggt tcagccattg gcgaagctga cggtgactcc ggaagatttt
     1081 ccgttcctgg cacagatccg tgggcatgac gacgagcggc tgcaggaata tgcaaccgcg
     1141 ttgcagaccg gcgcaccgtg gccgaaggat tcgtgaaaga tccaaaccac gctgagcgat
     1201 taggcatcaa ttcatatgga aaactacgaa aacaaattgc tgtcgcttca agtccaggta
     1261 atggagccgc cgaaaacgtc cataccgtat agcaagttac tggcgctggt ttccatcact
     1321 gaatctgctc ttttagaaaa agccctcaaa gagctggcac aggacttgcc gttcaaaacc
     1381 aattcctggc caagtttcgg cggttatgtc gaagattggt acatggtgga gttgagcgag
     1441 ttcaaacgca acctgctcta ttgtgagcgc ggcggctatg atctcagaca taaatgtgca
     1501 aatggaattg atctggtttt tgagatctta cggattattc tccccggcta taccgagact
     1561 gcaaggtcgg ttgctgcagg tctcgatcgg atagcagacc aaagactcct gctctggttg
     1621 cagatgagtt ccgagatata tcacatgtat caggtcaatg aatatttcgg ccttcgggtg
     1681 gcaacgtata acgtgacgtg gatgtgtcag gaattggtcc gcatgggtgt attcgcaaat
     1741 gaatcagagg cgttcgagag cgaagaacac attctcaagg gtttgatgag cagcggaatg
     1801 ccagatgaat tcgcgagaag gctgctgcat cagctaaagc atgcctagct gttcaccgga
     1861 ctgttaccat cgatgtgcct tcaaagaatt aggtggagac gaagtaaaac gagttgagga
     1921 tatattacgg gaacgacaag gactggcgag atgtagaaac ccttgcggcg ttgaatacac
     1981 gtcgtgagac cgttgaaatt gcgatctcac gcccgcctgg caaattgaaa catcaaaggg
     2041 agggtaataa agttctgggg tatcattccg actgaacata gctaatccgg ggtactaaat
     2101 acatccaata agtgataaag aggagcgtag tagatggcat ccacaaacac agacgcgcgt
     2161 aattccccca ctgagactca gtctgccggg gagcgccgcg tccatatcat cggggcggga
     2221 cctgtaggtc ttctccttgc agcattgctc cagccaatag aagggctcgc cgtccatctc
     2281 tatgagaagc ggcatgaata cacacgtact cgcatggtcc agctttcctc ttacctggta
     2341 gcagactcgg ttgagagcta tcgtacggat cacattgatg gggacaccat catcgcggtc
     2401 ttcgatccac ccgagatcga cgagggactc gcgttcaggc gggctatccc tgctgacctg
     2461 atgacacttc tgaagcaatg gacgctcggg ttcgttccac tcaacaccat cgaacgttcc
     2521 atcagcgatc tgatcgacgc gcgcaagacg aatacaatgc accgcacggc agccgtcgtg
     2581 acggcagagg aagcgataac catgctcagg ccgggagata tcctcatcga ttgcacgggg
     2641 accaagtcgc tccttcgaga tgatctgatt cccgattctg gtgaggtgcc aggtgcgaac
     2701 acgcttacga tccgactcga atacgcgctt gtcgtcgctt tcctgtacgg tcagaaatac
     2761 gattgcaacg aggtttgcaa gtacgctaaa aacattgaaa acccacagta caaatttatt
     2821 ccaatggtgc aacgcacgca ttacgacggc agtgtcaccc acgtgaccgg gatcgttaat
     2881 atcaccgcgg aggaatatga ggcaatgcca tctcgattcg atggtcaatg gctgcgcaac
     2941 aactttcccg ccccggctca gtctatggac cgtttcattg ataagatcaa ggaagaaacc
     3001 catggcgaaa tcctgggcga cctggagatc atccgcatcc ccctgaatct gtatcgggct
     3061 cgcaacgcta ccaaccggca gtggctcaaa acggagcacg ttaaccatcc atttgccagc
     3121 tcccccgtat tccttgcggg cgattcggct attggctctc cgtattttca atccatttcc
     3181 ctgggctttg agtgtgccat gtttctggct ggactcatcg cacaacctga cctgcctctg
     3241 aaagagatgc tcgatcgcta tgaactgcat acctataagc agtggcttcg agtctatatg
     3301 cgcagcaaga tgatcaagca caacaaaaat ctgtttgaat ccatcgataa caaagatgcg
     3361 ctgcttgatc agctacatat ctattagcaa agcacactga cgtaaaaagg caggagatgg
     3421 aacaagagct acgtatgcta aatcttcccc agccagggca acgactgaag atcacaaatt
     3481 ccgattacat tgtcatcagt gaagaatggg tcgtgctcac agtagagggc acgatcatca
     3541 ccttctcctg taaaaaatgg tgggactaca actcgatcac aatgcaaggg caaacctccg
     3601 agactgtgat cggggagatc gcgaaagctg atgataaaga agtgattatc aggttcacac
     3661 agtattcaag catcgctgtg gaagtagggc tggccatcca cggcgatcaa gcaacgtatc
     3721 tcaaacgcct cgacccgcca gcatatgcct gaaatagcac ttatccaaag gcgaggtttg
     3781 tagcaccaaa gacctttggt tcacaagcgg gcgattcgga tcatctgcga ttgtaacgaa
     3841 acaagatctg gtagaaacgc cataaaacag gagaaaccat ggacaacgat aatggattag
     3901 atgtggaata ttacacttcg gagcttctga agggattgcc attttcggcg gcagtccgcg
     3961 ttgggaagat gctgtatcta tccgggcaaa tcggcattga tcactctggc aaattggtag
     4021 caggaggcat cagtgccgag acgcgtcaaa ccatggaaaa tattcgagcc acgctggagg
     4081 aatatggctc atcacttgat catgttgtca aggctaccat catgttggct gagatgagcg
     4141 aatggtccga aatgaacaag gtttatatta cattttttag ccagcacttg cctgcacgca
     4201 gcgccctggg agcgaacggg ttggcattag gtgcccgggt tgaaattgag tgtattgcgg
     4261 ttctgcgata agatagtttc ctctcacctg atgaataacc aaaacatctc gattccactt
     4321 aagcgattcc tgctcatcga gggatgtccc gaagactgga gagggctgga cctctatcta
     4381 tttcgagata atgacgtagt gttttatgtc ggtcaatctc atctggcctt tgcccgcgtc
     4441 tggaaacatt tgctcagcgg cttcaaaggt cactcgatcg taggtcgctt cgtctggtgt
     4501 aattggccac ggtccatgag cttcaccatc gagttgttta gttcacaatc tgagcaattc
     4561 ggtgaggtcg ggaatgaatt gaacgcttct gaacgattgc tcatccagcg ctggtctcct
     4621 tgtttcaacg tatctcagaa cagccagccc accccggtgc cagctgctta tttgccaccc
     4681 aatgctccgt tcagacgcag acgcagctta ctcgccctga cccacgaagc ggcgcgagcc
     4741 gtgaaggccg aggatgcaaa gctttgggca cagaatgtag agtggtgatc gtctaagctg
     4801 tcaaacacag cccgacactg ctacaaacct cggcgttgct ttcgtccaat cagcatataa
     4861 acgggtttga tatactttaa ccatgatctt cgagttcact agcaagatta tttactggag
     4921 aggtcccgct ccatggttct tcgttacgat gccagccaaa cagtcacgtg aactaaaagc
     4981 ggtatcctca ctcgtgactt atggctgggg cgtgattcca gtgcacgttc ggatcggcaa
     5041 aaccgagttt acgacctcgc tgtttcctaa agatggccgc taccttgtgc cgatcaaagc
     5101 cagcgtccgc aaagcagaaa atattgaaaa aggcgacaaa gtcactgttc gcctcgaaat
     5161 tcactaaaaa ccaatcaata aagaataatg aactgcctcc ctggaattgt gttccagcgg
     5221 aggttttttt gatccaaatt caggaacatt ttggcgcccg ggttggtcta aaggaaaaac
     5281 cccactaaat tttgtattta ataaggagct caaaacatga aagcggtcca atttttcctt
     5341 tttgtcatcc tgagtacaat atttttatct gcctgtgcgg caacctcaga gcccgccgcc
     5401 accccatttc ccgagtcgat gaaaggctac gaactatata gctggcagga tggcagccaa
     5461 tggaaattct cgatcctggt tggaaccaat cgcgaaaaaa gcctggatga aatcaaaacc
     5521 gccgatgttg ctatctccgg tatcgaagag cttaaatcca cgcttgaaca gatcccagag
     5581 gggcaataca tcacctggtc cacgcgagaa acgctttcat tcccaccaga agaaatcctc
     5641 aaacaggttg agcaaacctg cagtgataaa ggactgatat tgaacatcgc tttataatcc
     5701 ctgcatgcaa acgggccgtg acaatttaga agagttcgat gatcctccaa actacgatat
     5761 tgaagaagct gagcgatctg ccgcacgcat cgcttattat tgtgaactcg ccaggtcaat
     5821 tggcggacct gtattggaga tcgcgtgcgg ctccgggctg gtagccatcc ctgttgcagc
     5881 acagggattg aacgtaaccg gcgttgacct ctcccgcccg atgttggaac atgcccgcaa
     5941 aaaagctgac gagcagcatc tgaacattcg ctgggtggaa gcagacgcgc gttcttttga
     6001 ccttggaaca cagtaccaat tcatttacct gaccggaaac gctttccagg cttttctatc
     6061 gcgcgaggat caggaaactc tgctcgcctc cgtgaagcgg catctcgctc ccgatgggat
     6121 cttcgcgttc gagacgcgca acccatcggg tcacgatctc tctgaccaac ccgagccgga
     6181 gttcgatcaa agctatgtca gcgtggaagg acatcaggtt tccgtctacg tttcgcagcg
     6241 atatgagcca cttacacagg ttatgtactg gacaacgttt cgccactgga atgatgggga
     6301 acgcgatcac accaaacaaa cacatatcgc ctgtcgcttt acgcatccac aggaattgga
     6361 aatgctctta cactacaacg gttttcaaat cattcaataa tatggagacc tggataagca
     6421 ggcgctttcg gcatccagcc ccagtatttt ttcgatctgc aaagttcgat agatggatgt
     6481 atgtcccatt tgaagccaga tgtatccttg tacggcataa cgaaattcat cgaggaacgt
     6541 tttctttaac gcctgattac ctggcacacg agcccgcgaa cctgcggccg ggcatctgtt
     6601 gttcagcggg tatagactgc aactctcccg tcagttccat cctacagcac tttgacggta
     6661 aatattttct gcgcattgcg atcgccaatc acagcagcag actggaagat tttgcccttt
     6721 ttgtgcgcga actcgttcga atcggccggg aggtgggctt ctaattcagt taactgattt
     6781 ctgcataaac atcgaatggg atgctgtaaa ctcctgagga ttggtcagaa tcgtgaggag
     6841 ttttatatt
//
LOCUS       DASTJP010000636         8259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1432519, whole genome shotgun sequence.
ACCESSION   DASTJP010000636 DASTJP010000000
VERSION     DASTJP010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432519"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VFQ23_18695"
     CDS             <1..994
                     /locus_tag="VFQ23_18695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9908684.1"
                     /translation="VPLILMSPFDWVRAPYKLLHSISNYKGTLMWLPNFAYNFTAQKI
                     RDRHLEGIDLSSLRAVINCSEPVRWESHQAFYERLKDYGLKYEALQTSYAMAENVFSV
                     TQSPLGSAPVVDEIDRESFMAERVVKGPFDGRPSLKMMSSGRPLANVKIKIVDEQGME
                     LPDCAVGEVILQSNCMLTGYYNRPDLSEKAFLNGWYLTGDYGYVSKGELFVAGRKKDM
                     IIVGGKNVYPQDLEMLAYEVPGVHAGRAVAFGMFDETQGTEEVVIIAEADSEDSDEQQ
                     KVADAIRLHVTKNSAIALRHVKVVGSQWILKTSSGKTARSANKEKFLKELQRDK"
     gene            complement(1037..1810)
                     /locus_tag="VFQ23_18700"
     CDS             complement(1037..1810)
                     /locus_tag="VFQ23_18700"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET9908685.1"
                     /translation="METQNIASETTLAPKTKLSGKVLKTTLAGALIDVGQSIPGVIHI
                     SQLSEGSVNKVEDVVREGQTVDVWVRRVKKDRIELTMIQPLGLEWKEIEPDMIVKGKV
                     VRLEAYGAFVDIGAERPGMVHVSELTHGYVKTPNEVVKEGDEVEAKVLDVNRKKKQIK
                     LSMKALEPVIEEFKPAKREDTKGPGRRGPRKEKPVEEVVPQEEPKEPELTGMQVAWQE
                     ALNKANAERAYKVKRSKSRSQEQDDILNRTLEKRLPTGG"
     gene            2095..2601
                     /locus_tag="VFQ23_18705"
     CDS             2095..2601
                     /locus_tag="VFQ23_18705"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET9908686.1"
                     /translation="MYNFDKTDIKIVNLLLEDGRMSASEIARRIGNISERAARYRIER
                     MVNEGVVRISAVAKPQAFGLTTIADVWMEVESDHILAVAKRMVEFDNISYVACGIGES
                     DVSIQIVAKDTAEIYHFVTEVVRKVPGVRKTTTSIVPLILKDVYQWRIPERIVYELSD
                     EEVPGNTD"
     gene            2619..3917
                     /gene="gntE"
                     /locus_tag="VFQ23_18710"
     CDS             2619..3917
                     /gene="gntE"
                     /locus_tag="VFQ23_18710"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis PLP-dependent
                     transaminase GntE"
                     /protein_id="HET9908687.1"
                     /translation="MFGEKTHALQKRAQEVMPLGVNSNFRFWGEGITPYVEKAKGAYL
                     WDVDGNKYIDYRMAFGPIILGHSYDEVDQKVIEEIQRGLLFAMTGELEVAVVEMIVEM
                     APAVEMVRLACSGTEATMHAIRVARAYTGRDLILKFEGNYHGFHDHTLWSTYAPVEAY
                     GNRRSPIPVPASSGIPKSMREFIITLPFNDFEGFERVMRSYGEQIAAVISEPCQGNCA
                     AINPQDGFLELIRKKTEEYGCVFILDEVKTGFRIANGGAQEHYGIKPDLATYAKALGN
                     GYPVAAFGGKKEIMSIIGQGVAQGGTYTNNKPGVAGAFATLSLLKSKPILKTIEKRGQ
                     RLMDGLKEIFEENDIPVVMTGYPAMFSFSLGVDSVTCQRDWALSDHDMYLKLTEKAIE
                     KGVMPDHDAREPWFLCYEHSDADVDETLNVYAEIVKEVKR"
     gene            4123..5430
                     /locus_tag="VFQ23_18715"
     CDS             4123..5430
                     /locus_tag="VFQ23_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9908688.1"
                     /translation="MTKLTSQEIIDLNKEFTFYSWSNQGQVNPIPMVKAEGPYFWDAD
                     GKRYLDFASQLVNTNVGHQHPKVIKAIQDQAAELTFAAPGMATEPRGLLGKKLAEITP
                     GDLKKTFFTLGGAEANENAIKIARFYTGRHKVLARYRSYHGATHGAMALTGDYRRLPA
                     EPAISGVVHFLDPYCYRCPFGWTKETCHRECISHVEEVIQHEGPDQIAAIFLEGVTGT
                     NGLIIPPDEYWPRMREICDKYGILLISDEVMSGWGRTGEWFAVNNWNTVPDIITTAKG
                     ITSGYVPLGAVIVREKIAKFFDDKMLYAGLTYSGHALACATALATIEAYEEDEIFENA
                     KKVGKYLGARLEELKARHPSVGDARYIGLFSALELVKNRETKEPMDLTPLKNFMIENG
                     VYVFNFKNILFIVPPLVITEEQLEEGLILLDEGLAELMDKMVN"
     gene            5479..6945
                     /locus_tag="VFQ23_18720"
     CDS             5479..6945
                     /locus_tag="VFQ23_18720"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET9908689.1"
                     /translation="MEILNYINGEWVKPDVQEYLDVINPATAQVIARTPLSSASDVDT
                     AAKAASAAFPAWRRTPVNDRVQYLFKLRDLMRSNHDEIARIITNECGKTFEEAKAEMV
                     RAFENIEVACGMPMMMKGEVVEDIAPGIDEIMLRQPVGVCATIAPFNFPGMIPFWYLP
                     YAIACGNTYIIKPSEKVPMTMQFIMKLIEQVGLPNGVINLVNGGKETVDAILDHPIIR
                     AVTFVGSSATAKYLYGRAASNGKRVQAQGGAKNAVIVLPDADMDMATKIIADSAFGCA
                     GQRCLAVSVAVTVADARNQFVEMICDAASSRTVGYGLDDGVQMGPVINAASKARVEHL
                     IGVGAEAGATVPVDGRGAVVKGYEGGNFVKPTILLDVPRGSEVSRTEIFGPVLSLMHV
                     DNVEDAIELVNSGNYGNQASLFTSSGNAARRFRYETEAGNIGINIGVAAPMAFFPFSG
                     WKESFYGDMHGQGMDAVEFFTQKKVVVERWPKEWTRKF"
     gene            7020..>8259
                     /locus_tag="VFQ23_18725"
     CDS             7020..>8259
                     /locus_tag="VFQ23_18725"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9908690.1"
                     /translation="MNNYNNALSYSSQWLDIINQQNFPEESQAKWIIDESKQNFAEHF
                     NRGWLEYRKSVTEAGDWASVEWSGKGSTFTDVIGRTYIDWLGGFGSLDLGWCHPDVVE
                     AVVAQVRRSPMPSQELIDPLRGVLARLMAEITPGDLKYSWFAASGTEAIEAAIKIAKL
                     YTGKSAFIVAVKAFHGKTMGSLSMMGKADYRQPMGLLYGGNVYHVPFGDADAVERQLE
                     ICDKVGIGVAGVMFEPIQGEAGAIVPPDDFWPRVRDVTKKYGVLLIADEVQTGLGRTG
                     QLWGVNHWDVTPDILATAKSLGGGVMPVSTAITTEEIFQPMMYPNPFMHTTTTGGNAL
                     ACAAAIATINVILRERLWESAATKGVYLKEKVDDIAQEFPHIYKEVTGKGLLIGQHFH
                     TPEIGYKVAAELFKRRVLVAG"
BASE COUNT         2101 a         1846 c         2225 g         2087 t
ORIGIN      
        1 tgttccgctg atcctgatgt cgccattcga ttgggtacgc gctccctaca agttgctgca
       61 ttccatttcg aactataaag gcaccctgat gtggctgcca aattttgctt acaactttac
      121 cgcacaaaaa atccgcgatc gtcatttgga gggtattgat ctttcatctt tgcgagcagt
      181 catcaactgt tctgagcctg tgcgctggga aagtcatcag gcgttttacg agcgactcaa
      241 agattatgga ctgaaatatg aggcgctgca gacttcctat gcaatggcag agaacgtctt
      301 cagcgtgacc caaagtccat tggggagcgc gccagttgtc gatgagattg atcgagagtc
      361 atttatggcg gagcgcgttg tgaaaggtcc gtttgatgga cgtccatcct tgaagatgat
      421 gtcctccggt cgccctcttg caaatgtcaa aataaaaatt gttgatgaac aagggatgga
      481 attaccagac tgtgcagttg gggaagtcat tcttcaaagc aactgtatgc tcactggtta
      541 ctacaatcgt cctgaccttt cagaaaaagc ttttctcaat ggctggtatt tgaccggtga
      601 ttacggatac gtgtcaaagg gcgaactctt tgttgccggt cgtaagaagg atatgatcat
      661 tgtgggtggg aagaatgtgt acccgcagga tctggaaatg ctggcgtatg aagttcccgg
      721 tgtacatgct ggcagggcag tggcgttcgg catgttcgat gaaacccagg gcaccgaaga
      781 agtggtcatc atcgctgagg cggattcgga ggattccgac gaacaacaaa aggtcgcaga
      841 tgcaatccga cttcacgtaa caaagaattc agcgattgcg ttgcgccacg tcaaagttgt
      901 ggggtctcaa tggatcttga agacgtccag tgggaaaaca gctcgttctg ccaacaaaga
      961 gaaattctta aaagaattgc agagagataa atgaaaaaac agtcccgcca aatgggactg
     1021 tttttttttt cagactttat ccaccagtcg gcaatcgctt ttcgagcgtg cgattgagaa
     1081 tgtcgtcctg ttcctgtgac ctggatttcg agcgtttaac tttgtacgct ctttcggcgt
     1141 tggccttatt gagcgcttcc tgccatgcca cctgcatgcc agtcagttcc ggctctttcg
     1201 gttcttcctg agggacaact tcctccacag gcttttcttt ccttggaccg cgccttccgg
     1261 ggcccttcgt atcttctcgc ttcgcgggtt tgaactcttc gatgactggc tcaagcgctt
     1321 tcatgctcag cttgatctgt ttctttttgc gatttacgtc gagcaccttt gcctcgactt
     1381 catcgccttc cttgaccact tcatttggag ttttcacata gccatgtgtc aattcactga
     1441 catgaaccat acctgggcgt tcggctccaa tgtcaacaaa tgctccatac gcttcaagac
     1501 ggaccacttt gccttttacg atcatatctg gctcgatctc tttccattcg agccccagag
     1561 gctggatcat ggtcagctcg atgcggtcct tctttacgcg tcggacccac acatcgactg
     1621 tttgtccttc acgaaccaca tcttctactt tgttaactga cccttcactc aactgcgaga
     1681 tgtgaatgac gcctggaatg ctttgaccca catcaattag tgctccagcc agggtggtct
     1741 ttaatacttt tccgcttagt tttgtctttg gcgcgagcgt cgtttcagac gcgatgtttt
     1801 gtgtttccat aatgtgctcc tgcaagattg ggatgatgcc gaaaaataat tatacctatt
     1861 tgataaatat cagtcaatgt gaaataggtt cttaatttta tttagatggc tcggtaatta
     1921 aattttatag acgtatacca agctatatat tcctagttca atacgcatgc ctaactacaa
     1981 gacgcggtta ataccccgat ctattacgtg aacaaatacg tacttgaccg tttcaagcct
     2041 cgtaatgtat atttggctta cgcatgtcga tttcggaaac aaaaatgagt aattatgtac
     2101 aatttcgaca aaactgacat aaaaattgtc aatttgctat tggaagacgg ccgaatgtct
     2161 gcttctgaga tcgcgcgtcg cattgggaat atttcagagc gcgccgctcg ctatcgaatt
     2221 gagcgcatgg tcaatgaagg ggtggttaga atcagcgcgg tggcgaaacc ccaggcgttt
     2281 gggttaacca cgattgcaga cgtctggatg gaagtggaat cagatcacat cctggctgtt
     2341 gcgaagcgaa tggtggagtt cgacaacatt agttatgttg cctgtggcat tggggaatca
     2401 gatgtaagta ttcagatcgt tgcaaaagac accgcagaga tttatcactt cgttactgaa
     2461 gtggttcgta aagttcccgg cgtgcgtaag acgaccacat ccatcgtgcc tctcattctt
     2521 aaggatgtgt atcaatggcg aatcccggaa cgaatcgtgt atgagttatc agacgaggaa
     2581 gtccctggca atacagacta attaaactgg agaactctat gtttggtgag aagacgcatg
     2641 cattacaaaa acgcgcacag gaagtaatgc cgcttggtgt aaattcgaat tttcgtttct
     2701 ggggtgaagg aatcacaccc tacgttgaaa aagcaaaagg ggcatacctg tgggatgtgg
     2761 atggcaataa gtatattgat tatcgtatgg cgttcggtcc gattattctc ggccactcgt
     2821 atgatgaagt cgatcagaag gtaatcgaag aaattcaaag gggccttctc tttgcaatga
     2881 ccggcgaact cgaagtcgca gttgtggaga tgatcgttga gatggctcca gcggttgaga
     2941 tggtccgcct ggcgtgttcg gggacagaag cgaccatgca tgcgatccgt gtggcgcggg
     3001 catatacagg tcgtgaccta attctgaaat ttgaaggaaa ctatcatggg tttcatgacc
     3061 ataccttatg gtcaacttac gcgccggtgg aagcctatgg gaatcgacgc agtccaatcc
     3121 ctgtgcctgc ttcatcaggt atcccaaagt ctatgcgcga atttattatc acgctgccgt
     3181 tcaatgattt tgaaggcttc gaacgtgtga tgcggtctta tggcgaacag atcgcggcgg
     3241 tgatttccga gccctgccag ggaaattgtg cggcaattaa tccacaggat ggttttctag
     3301 agttgatccg caaaaagaca gaagaatatg gatgcgtatt tattctggat gaagtaaaga
     3361 ccggatttcg cattgcaaat ggtggtgcac aggagcacta cggcatcaaa ccggacctcg
     3421 cgacgtatgc aaaagccctc ggcaacggct accctgtcgc ggcgttcggc ggcaagaaag
     3481 agatcatgtc gattatcggg caaggggtag cacaaggcgg cacatacacc aacaacaagc
     3541 ccggggtggc gggtgcattt gcgacgttga gcttgttgaa gtcaaaacct attttgaaga
     3601 ccattgaaaa acgtgggcaa agattgatgg atgggttgaa ggaaatcttc gaagagaatg
     3661 atattccagt tgtgatgacc ggttaccctg ccatgttctc gttttcttta ggcgttgact
     3721 cggtgacgtg ccagcgcgac tgggcattaa gcgatcatga tatgtatctc aaattaacag
     3781 agaaggccat cgaaaaaggc gtaatgcccg accatgatgc gcgcgagccg tggttcctgt
     3841 gttatgaaca ttcggatgcg gatgttgatg aaacgttgaa tgtgtatgca gagattgtga
     3901 aggaagtgaa aagataacaa atacactcgg tggttgcgta gacccgagct gtctttgcga
     3961 aggttgtaca cttgcgctgg cacgtgattt gcctggcaaa tcctgcaggt gtcgaaacca
     4021 ccagttgcaa atggtatttt gtaacaagat tactctgttc acatggtggc ctcgatacgg
     4081 acgaaacagg cactcgtcct actcgaccac cgaggaattc caatgaccaa actaacctcc
     4141 caggaaatta ttgatctcaa caaagaattc accttttact cctggtcgaa tcagggacag
     4201 gtgaatccga ttccgatggt taaggcggaa ggtccttatt tttgggatgc agatggcaaa
     4261 cgctatctcg attttgcgtc acagttggta aatacaaatg ttgggcatca acatccaaaa
     4321 gtaatcaaag cgatccagga tcaagccgcc gagttgacct ttgctgcgcc cggcatggcc
     4381 actgaacctc gcggcctgtt aggaaaaaaa ctggctgaga tcacgccggg agatctgaag
     4441 aaaacgttct tcacgctagg tggggcagaa gcaaacgaga atgccatcaa aattgctcgt
     4501 ttttatacgg gcaggcataa agtccttgcc cgctatcgtt cgtatcatgg tgcgacgcac
     4561 ggcgccatgg ccctgacagg tgactaccgt cgcttaccag ctgagcctgc gatctcgggt
     4621 gttgtccatt ttcttgaccc ttattgttat cgctgtccgt ttggctggac aaaagagacc
     4681 tgtcatcgtg agtgcatcag tcacgtcgaa gaagtgattc agcacgaggg tccagaccag
     4741 atcgcggcga tcttcctcga aggcgtgact ggcacaaacg gcctcattat cccgcccgat
     4801 gaatattggc cgcgaatgcg tgagatctgc gataagtatg ggattctatt gatctcagat
     4861 gaagtgatga gcggatgggg acgcacgggg gaatggttcg cagtaaacaa ctggaatacg
     4921 gttcctgaca ttatcacaac tgcaaaagga attacttctg gctacgtgcc acttggcgcc
     4981 gtaatcgttc gtgaaaaaat tgcgaaattt tttgatgaca agatgttgta tgccgggttg
     5041 acatattccg gtcatgcgtt ggcttgtgct acggcactgg cgaccattga agcctatgaa
     5101 gaagacgaga ttttcgagaa cgcaaagaaa gttgggaaat atctcggggc gcgattggaa
     5161 gagctaaaag ccagacatcc ctccgtgggt gatgcgcgct atatcggcct tttctccgcg
     5221 ttggaactgg taaaaaatcg cgagacgaaa gaaccaatgg atttgactcc gctgaagaat
     5281 ttcatgatcg agaacggtgt gtatgtgttc aattttaaaa atatcctgtt tatcgtcccg
     5341 cctctggtga ttacggaaga acaactggaa gaaggattga tccttttgga tgaaggattg
     5401 gctgaattga tggataagat ggtgaattga tgtgtagtag ggacatagca ctactgtgtc
     5461 ctcacaggag atgaaaagat ggaaatactg aattacatca atggtgaatg ggtcaagccc
     5521 gatgtgcagg aatacctgga cgtcatcaac ccggcgacgg cgcaagtcat cgctcgcacg
     5581 ccgctttcca gcgcttccga cgtagacacg gcggctaagg cggcaagcgc cgcgttccct
     5641 gcgtggcggc gaactccggt aaatgacaga gtacagtatc tgttcaaatt acgtgacttg
     5701 atgcgctcca atcacgacga aattgcccgc atcattacga atgaatgtgg caaaactttt
     5761 gaagaggcca aggcagaaat ggtgcgcgcc ttcgaaaata ttgaagtggc atgcgggatg
     5821 cccatgatga tgaagggtga agttgttgag gatattgctc cgggaatcga cgagatcatg
     5881 ctacggcagc cagttggtgt gtgtgccaca atcgcaccgt ttaacttccc gggcatgatt
     5941 ccgttctggt atttaccgta cgcgattgcc tgtgggaata cctacattat taagccatct
     6001 gaaaaagtgc cgatgacgat gcagttcatc atgaaactga tcgaacaagt tggtttgcca
     6061 aatggtgtga ttaatttagt caatggtggc aaagaaactg tggacgcgat tttagatcat
     6121 cccattatcc gcgcggtcac gtttgtgggt tcgagcgcga cggcgaaata cttatacggg
     6181 cgcgccgctt ccaatggaaa acgtgtgcag gcacagggtg gagcaaagaa tgcggtcatc
     6241 gttttgcccg atgccgatat ggatatggca acaaagatca ttgccgactc ggcgtttggt
     6301 tgtgcgggtc aacgatgtct ggctgtatcc gtcgccgtga ccgttgcaga cgcgcgcaat
     6361 cagtttgtcg agatgatctg tgacgccgcg tcaagtcgca ccgtcggata tggactggat
     6421 gacggagtcc aaatgggacc ggtgatcaac gccgcgagca aagcgcgggt cgagcatttg
     6481 atcggggtcg gcgcggaagc cggagccact gtcccggtgg atggacgcgg ggcggttgtc
     6541 aaaggatatg aaggaggaaa cttcgtcaaa ccgaccatct tgctggatgt tccgcgtggc
     6601 agcgaagtct ccagaaccga aatctttggc cctgtactga gcttgatgca cgttgataat
     6661 gtagaggacg ccattgaact ggtgaacagc ggcaattatg gaaatcaggc cagcctgttt
     6721 acctcaagtg ggaacgccgc acgcagattc agatatgaaa cagaagccgg aaatatcggc
     6781 atcaatatcg gagtcgccgc gccgatggca ttcttcccct tcagcggatg gaaagagtca
     6841 ttttacggtg atatgcacgg gcagggcatg gacgccgtcg aattcttcac tcagaagaag
     6901 gtcgttgtgg agcgatggcc gaaggaatgg acgaggaaat tttaggaaac gcctggtggt
     6961 cgagtaggcg gcgtctgtcc gccgtatcga gaccaccgaa atattactgc aaggaattta
     7021 tgaataatta caacaacgca ttatcatatt cgtctcaatg gcttgatatt atcaatcaac
     7081 aaaactttcc tgaagaatcg caggcgaaat ggatcattga tgagtcaaag caaaattttg
     7141 cggagcattt caaccgtggc tggctggagt atcgcaagtc ggtgacggag gcgggggatt
     7201 gggcatcagt tgaatggtcg ggcaagggct cgacatttac cgatgtgatc ggtcgcacat
     7261 atatcgactg gctcggtggc ttcggaagtc tggacttggg ctggtgtcat ccggatgttg
     7321 ttgaagctgt ggtggcgcag gtgaggcgaa gcccgatgcc cagccaggaa ctcatcgatc
     7381 cattgcgggg tgtacttgcc aggctcatgg cagagatcac tcctggcgat ttgaaatact
     7441 catggttcgc cgccagcggc acagaggcaa tagaagctgc gataaagatc gccaaactct
     7501 atactggcaa gtctgcattt attgttgctg ttaaagcatt tcatggcaaa acgatgggtt
     7561 cgctttcgat gatgggcaaa gcggattatc gtcagccaat gggcctgtta tatggcggaa
     7621 atgtttatca cgttccgttc ggcgacgcag atgcggtgga acgtcagctc gagatatgcg
     7681 acaaagttgg aattggtgtg gcaggtgtga tgttcgagcc catccaggga gaagcgggcg
     7741 cgattgtccc gcctgatgac ttctggccgc gtgtgcgcga cgtgaccaag aaatatggtg
     7801 ttctactcat tgccgatgaa gtgcaaacgg gcctggggcg gaccggtcaa ctgtggggtg
     7861 taaatcattg ggacgtcaca ccagacatcc tcgccacggc caaatcgctt ggtggaggcg
     7921 taatgcccgt cagcacggcc attactacag aagaaatctt tcagccgatg atgtatccga
     7981 atccttttat gcacacaact acaaccggag gaaatgcact agcatgcgcc gccgcgatcg
     8041 ctaccatcaa tgtcattttg cgtgagcgat tatgggaaag cgccgcgacc aagggcgtct
     8101 atctcaaaga gaaagtggat gatatcgcac aggagtttcc gcacatttat aaagaagtaa
     8161 caggtaaggg attgctcatc ggccagcatt ttcatactcc cgaaataggt tataaagtcg
     8221 cggcagaatt gttcaagcgc cgtgtactgg tagccggca
//
LOCUS       DASTJP010000637         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1342251, whole genome shotgun sequence.
ACCESSION   DASTJP010000637 DASTJP010000000
VERSION     DASTJP010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342251"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFQ23_18730"
     CDS             complement(<1..191)
                     /locus_tag="VFQ23_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908691.1"
                     /translation="MPYARKNLLIWQKGMYYHIYNRGARGLRIFREPRNYLFAISKFR
                     EFSLVNDIKVIAYCLMPTH"
     gene            complement(388..1632)
                     /locus_tag="VFQ23_18735"
     CDS             complement(388..1632)
                     /locus_tag="VFQ23_18735"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9908692.1"
                     /translation="MNTYLSFLQLRPKFRSLWLAQVISLSGDWFNTIASVIIINRYAP
                     SGLAVGGLFIARALPPFLLGPLAGVVADRFDRRKILILSDLLRACIVLGFLLVDRPER
                     LWLLYVLTVLQFSVSTFFEPARAALVPALVESDELLKANTLASITWSAMLTLGGAIGG
                     LTASLFGVRVSLIVDALSFLVSAFFLFSITGQYRSEAVHALESGWKNFIDGLRYVRQH
                     LDVGLVTSVKALGQVGSFDIIAALYAVHVFREGQEGATTLGLMFTAFGVGAVIGPLIA
                     NQFGDSSVVWLRRAILGGFVCMPLAWLIVGVAPSLPIALLGCILRGIGGSVNWTYSDV
                     ILQMSVPDHLLGRVFAFDFGIFTLAVSVSLWLTGFITDTFQLAPRVIVLLLAAGSLGP
                     LAVWGAALRWQARRVVQESMNG"
     gene            1745..2125
                     /locus_tag="VFQ23_18740"
     CDS             1745..2125
                     /locus_tag="VFQ23_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908693.1"
                     /translation="MNSTRQEKLSGSALVDFLWRSENLTDGVYVASADASWDMIFTRT
                     RAGQPKVLLCGPYYKTAQVPFCSGEKHIGICFKPWAVFTGIPMTTMLNSGEFLDSPSE
                     DTFVMQGITWNFRPTRHSPIFPRA"
BASE COUNT          626 a          586 c          581 g          481 t
ORIGIN      
        1 taatgagtag gcatcaggca ataggcgatt actttgatat cgttgaccag gctaaactcc
       61 ctgaatttgg aaatagcgaa aaggtaattc ctaggttcgc gaaagatcct tagcccacgt
      121 gcaccgcgat tgtagatgtg atagtacatc cctttttgcc agatgagtaa attttttcga
      181 gcgtatggca acgttactcc gaacaatagg tgacagtcac tgtgtcccct cagttgtcct
      241 tcggggatgc tgcgcaaaag tgactgtcac cttaatgcat acagaattct atgtttccgg
      301 agaagatgag tcaaccaaca atgttgaaat catgaggtac gaaatgacag tcatctatgt
      361 tatgttgtca ctgtcatttt ttttgcctca cccattcata gattcttgca ccacccggcg
      421 ggcttgccag cgcaacgcgg cgccccacac tgctaacgga cccagactcc cggctgccaa
      481 caacagcaca atgacgcgtg gggcgagctg aaacgtatcg gtgatgaaac ccgtcagcca
      541 gagcgaaacg gacactgcca gcgtaaagat cccaaaatca aacgcaaaca cacgtccgag
      601 cagatgatca ggcaccgaca tttgcaggat cacatcacta taggtccaat tgacggagcc
      661 accgataccg cgcaagatac atcccagcaa agcaatgggc agagagggtg ccaccccgac
      721 gatgagccac gccagtggca tgcagacgaa gccacccagg atggcgcgtc gtaaccagac
      781 tacactcgag tcgccgaact gatttgcaat cagcgggcca ataactgcgc ccacaccaaa
      841 ggcggtgaac attaagccaa gagtagtcgc cccttcctgt ccctcgcgaa aaacgtgaac
      901 cgcgtacagc gcggcaatga tgtcgaagct gccaacttgt cccagcgctt tgaccgacgt
      961 gaccagcccg acatccaggt gctgacgcac atatcgcaag ccgtctatga aattcttcca
     1021 gccgctttca agtgcatgca ccgcttcaga acgatattgg ccggtgatgc tgaataagaa
     1081 gaaagccgaa accaaaaaac ttaacgcatc gacaataagg gagacgcgca cgccaaagag
     1141 agaggcggtc agcccaccga tcgcaccacc cagcgtcaac atcgcagacc aggtgatgct
     1201 ggccagggtg ttcgctttga gcagttcgtc agattcgacg agggctggga cgagcgcagc
     1261 acgtgccggt tcaaaaaaag tcgagacgct gaattgcaat accgtcaaca catacagtag
     1321 ccacagccgt tccgggcgat ccaccagcaa aaagcccagg acgatgcagg cgcgcaagag
     1381 atcgctcagg atcaggattt tgcgccggtc gaatcgatct gcgacaacgc ctgccagggg
     1441 tcccagcaag aatggaggca aagcgcgggc gataaacaag cctcctacag ccagcccgga
     1501 gggcgcataa cgattgatga tgatcacgga ggcaatagta ttgaaccagt cgccgctgag
     1561 gctgataact tgtgccagcc atagggatct aaatttaggt cgaagttgga ggaaggagag
     1621 ataggtgttc acgcgaacaa ttatatctgc cggacgtttc tggttttaca aacagcatgc
     1681 tggttgaaga cgtataataa gcacggcgat gttcggcata actgtcctga aggaggcatg
     1741 atctatgaac tctacgcgcc aagaaaaact atcgggatct gctctggttg attttctctg
     1801 gcgctcggaa aatctgaccg atggagtgta tgttgcatcg gcagacgcca gttgggacat
     1861 gattttcaca cgaaccaggg ccggtcagcc aaaagtcttg ctgtgtgggc cttactataa
     1921 aacagcacaa gtcccctttt gttcggggga gaaacatatt ggtatttgtt tcaaaccctg
     1981 ggcggtgttt accggtattc cgatgacaac catgctcaat agcggagaat ttctagactc
     2041 accttcggaa gatacgtttg tgatgcaggg cataacctgg aatttccgac ctacgagaca
     2101 ttcgccaatt tttccgcgag cttaggtagt tatcaaagtg gcatatggga tgtgccgcca
     2161 taaaatgaaa gtcacccctt ttgtggggtg acttttgcct ggtttgcaaa tgaaaaccta
     2221 tctccggctg attccccttg tcgtaacatc aaatacggcc agatcaaaac gagt
//
LOCUS       DASTJP010000638         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_157680, whole genome shotgun sequence.
ACCESSION   DASTJP010000638 DASTJP010000000
VERSION     DASTJP010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157680"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="VFQ23_18745"
     CDS             complement(<1..1590)
                     /locus_tag="VFQ23_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET9908694.1"
                     /translation="MSDTRIAAYQLVDYLDQLGVEVIFGLTGHTVIALLDALGRCEKV
                     RYISTRHEQIAAHAADGYARASRKPGVLLTHLGPGLTNAATGVANAALDSIPMVVIAG
                     DVPSYYFGRHPHQEVNLHMDGDQYEIYRPFCKRIYRVDRVNDFPRIVERAFHLSQTGR
                     PGPVLVDVPMDMFSADLPIGAFNKTPAPMSKSTIDAATAEQVARALAEAERPVLYVGG
                     GVHGACASDELTALAEALEIPVAHTLMGKGSMSDNHPLLLGMTGFWGTPIANEMCRTA
                     DLIVAVGTRLAEANSSSWDPRYTFSIPPTRLIHIDIDPAEIGRNFPTELGIVADAKNA
                     LGMIAKAAQGIATPKRAGLREKIAKGRKEFASNWADQWSSDQYPLRPERILSEVRKAL
                     PEDGFLVTDVGWNKNGVAQQFPITIPGTFVTPSGLATMGFGPAAALGVKIAQPDRSVL
                     ALVGDGAFSSHMSVVATAMEAEIPVVWVVMDNAAFGTIAGLEKAHYGWTFGNLFESEG
                     KEYRVSYAEVARACGAQGVFIR"
BASE COUNT          640 a          578 c          534 g          622 t
ORIGIN      
        1 acggataaag actccctgcg cgccacaggc gcgggcgact tcagcataac ttacccgata
       61 ttctttcccc tcactttcga acagatttcc aaaagtccag ccatagtgtg ccttttcaag
      121 tccggcaatc gttccaaacg cggcgttatc catcaccacc cataccaccg gaatttccgc
      181 ttccatcgca gtagctacga cagacatatg gctgctgaag gccccatcgc ccacaagcgc
      241 cagaactgag cgatctggct gcgcgatttt gacacccaag gcggccgctg gaccgaatcc
      301 cattgtggcc agaccacttg gcgtgacaaa cgttcctgga attgtgatcg gaaattgttg
      361 agcgaccccg tttttattcc agcctacatc ggtaacaagg aaaccatcct caggcaatgc
      421 ctttctgact tcgctcagaa tgcgttctgg tcgtaaagga tattgatctg agctccattg
      481 atcggcccaa ttggaagcaa attctttacg tcctttggca atcttttcgc ggagaccagc
      541 gcgtttagga gtcgctatac cctgtgctgc tttcgcaatc atgccaaggg catttttggc
      601 gtcggcaaca attcccaatt ctgtggggaa attacgaccg atttcggctg gatcgatatc
      661 tatatgaatt aatcgagttg gtggaataga gaatgtataa cgtggatccc acgaactgga
      721 attggcctca gccaatcgtg taccgacagc tacgatcaaa tccgctgttc tgcacatttc
      781 atttgcaatt ggtgttcccc agaagcctgt catacccagt aaaagtggat gattgtcgct
      841 catgctgccc ttacccatca gagtatgggc taccggtatt tccaatgctt cagctaaggc
      901 ggttaattca tcacttgcac acgcgccatg aacacctccg cccacataca aaacgggccg
      961 ttcggcttct gcaagagctc gggcaacctg ttcggccgtt gctgcatcaa tagtcgattt
     1021 cgacattggc gccggagtct tattgaaagc accaatcggc aagtctgcgg agaacatatc
     1081 cattggtacg tcaacgagta caggcccagg acgtccagtc tgagaaagat gaaacgcacg
     1141 ttccacaata cggggaaaat catttacccg atctacacgg taaatgcgct tacagaaggg
     1201 acgatagatc tcatattgat caccatccat atgtaagttg acttcctggt gagggtgacg
     1261 accgaaataa taagacggaa catctcctgc tatgaccacc attgggattg agtctagtgc
     1321 cgcatttgca accccggtgg ctgcatttgt cagccctggg ccaaggtgag tcaacaatac
     1381 tcctggtttc cttgatgccc gcgcataacc gtctgctgca tgcgcggcaa tttgctcatg
     1441 gcgagttgag atatagcgta ctttttcaca gcgacctaat gcatccagca gtgcaatgac
     1501 agtatgacct gtcaagccga agatgacctc aactcctagt tgatccagat aatcaaccag
     1561 ttgataagcg gcaatacgag tatctgacat ggcttttatg aatctctttc ttgcattgat
     1621 atgtttgatc caaaagagta gaaattcagt acatgcttgg tgcttctggc aaataaaaaa
     1681 gcgagctgtg ccttgccaga attaacttat tcaacagggc atcactcgct ttctcacaat
     1741 cttaggattg ctgtcctatg ctcaacaacc agcagtgacc atttagacag cctgacacat
     1801 ctgaatatcg cccatccagg ctgaagggag gatatcgggc cttgcagcat ttgtgtctca
     1861 gctagaggca taaaccttta cctagtgtct catattgtgg gacagtgtct catgttcttg
     1921 tgttattata gctttgttgt atagaaaaat caagagatga acttttgata taaaagccat
     1981 tactatagag ctgttgatac agagttctcg atactctgct gaattctttt cgtggcttct
     2041 tccatgcact agcctcaggg taacaagcag aagcgtcgga gatgaatctt gacccgtgct
     2101 tttgaaatat cctgcacaaa atcatgaaac aggtgaaacg tggctgatct caatcgaaca
     2161 ctattggacc tatttcgatc ttcggatggc ttactactgc cagacgaatc agttcttgaa
     2221 gaaatctcaa aaaaacaaca tgagcttaaa gtctggaacg aagaatagta gttcaggggt
     2281 taattacaat gaaggaatta ctccaagagt tggcaaatca aatatatttg gtaaaatcaa
     2341 tagatgaaat ggagtgggac tttagcgaga gttt
//
LOCUS       DASTJP010000639         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1500889, whole genome shotgun sequence.
ACCESSION   DASTJP010000639 DASTJP010000000
VERSION     DASTJP010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500889"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFQ23_18750"
     CDS             complement(<1..789)
                     /locus_tag="VFQ23_18750"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET9908695.1"
                     /translation="MTTPHTHSPTLLKPAKPVGIIGYGAYVPRYRLPAKEVARVWTGG
                     KGGLPIKEKAVPGLDEDVITMSIEAARNAMKRARIDPTELRAVWVGSESHPYAVKPTS
                     TLVAEAIGAVPNTQAADWEFACKAGTEALVAAMGLVGSGMGKYAMAIGMDTAQGKPGD
                     ALEYTAGAGGGAYILGPAEESLAIINASYSYVTDTPDFWRRSDAKYPEHGMRFTGEPA
                     YFKHVSQAALHLMEASGTTAKDYKWAVFHQPNTKFPQRVASQLGF"
     gene            complement(786..1124)
                     /locus_tag="VFQ23_18755"
     CDS             complement(786..1124)
                     /locus_tag="VFQ23_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908696.1"
                     /translation="MLDLFLIGYVQVAAENIQATGDKTWLLRGLVVSSIENSLRDQRD
                     NAFSLAYLYVMAEEKNLNPNSEFHEIAELSSDEISSGGSISMKELIKISRILLAKHIT
                     IGKNLVRNNK"
     gene            complement(1491..1982)
                     /locus_tag="VFQ23_18760"
     CDS             complement(1491..1982)
                     /locus_tag="VFQ23_18760"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET9908697.1"
                     /translation="MNDHSVKSENLLPDLQLAYDDFSTCILSLPDSLLLSEMNGWSPR
                     DVVAHLIGWNKRMIEASSSILNGQTPSYYDDALNDYRNINAQMVITFSSQSKEELLAE
                     LESSMNTFRTFIASLPPNDLWDSHGVFHYRGRPATIAGVIQSLMQDYQHHTLEIQQWR
                     ENL"
     gene            complement(2151..3392)
                     /locus_tag="VFQ23_18765"
     CDS             complement(2151..3392)
                     /locus_tag="VFQ23_18765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9908698.1"
                     /translation="MTHRKLHYAWIVVAITFLVLLVAGGVRTAPPVLIKPLEQEFGWT
                     RANISFAIAVSILWFGLGGPLAGILVNRFGLRRMMTGGLILVAMGLSAMLKINTLWQL
                     HLFWGVLVGIGTGALANVLGAIVAQRWFNQHRGLIVGVMGAASAAGQLIFLPTMIALT
                     TWGGWRGAILIIAVAVAGVVIPVVWLMREFPRDKGLNPYCDTDTPTTLAEVADAARQT
                     SLAEAARTTDFWLLAASFFVCGYTTNGLIGTHLLPHAIEHGFDSASTANAIVLMGLMN
                     IVGTLASGWFSDRYDNRMLLAVYYSFRAASIAFLPFISDMSGLFIFAFVYGLDWIATV
                     PPTINLTAQRFGRASVGVLYGWIFFSHMLGAAVAAYIGGVLRDLLGDYTVAFFSAAVL
                     GFIAAGFSMTIKRMHPVMAEG"
     gene            complement(3502..>3682)
                     /locus_tag="VFQ23_18770"
     CDS             complement(3502..>3682)
                     /locus_tag="VFQ23_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908699.1"
                     /translation="QWCKRNNLERGETLTLLQVWDLSKLWYGNRLSIDYHGRSLEQVA
                     EVFRQAGLTSKFWFV"
BASE COUNT          961 a          923 c          882 g          916 t
ORIGIN      
        1 aaagccgagt tgagacgcta cacgctgtgg gaacttcgta ttgggctggt ggaagacggc
       61 ccacttgtaa tcctttgcgg tcgtgccgga ggcttccatt aaatgtaagg cggcttgaga
      121 gacgtgtttg aagtaagccg gctcgccggt gaagcgcatt ccatgctcgg ggtattttgc
      181 atcggaacga cgccaaaaat caggggtgtc ggtaacgtag gaatatgagg cgttgatgat
      241 cgccagcgac tcttctgccg gaccgagaat atacgcgccg ccgcccgcgc ccgccgtgta
      301 ttcgagcgcg tcgccaggct tgccttgcgc ggtatccatg ccgattgcca tggcatattt
      361 gcccatgccc gaacctacca agcccatcgc agcgaccaac gcttcggtgc cggctttaca
      421 ggcaaattcc cagtccgcgg cttgcgtgtt agggactgcg ccgatcgctt ccgcgaccag
      481 agtggaggtg ggcttcaccg catacgggtg ggattccgac ccaacccaga ccgctcgcaa
      541 ttccgtcggg tcgattcggg cgcgtttcat cgcgttgcgc gccgcttcaa tggacatggt
      601 gatcacgtct tcatccagcc cagggacggc tttctccttg atggggagtc cgcctttgcc
      661 accggtccat acgcgcgcga cctccttcgc tggcaggcga taacgcggta catacgcgcc
      721 gtagccgata atgccaacgg gtttggctgg tttcagtaaa gtgggggagt gtgtgtgagg
      781 ggttgtcatt tattgttcct gactaaattc tttccaatcg ttatatgttt tgcgagcaat
      841 attcgggata tttttatcag ttctttcata gagatacttc ctccagaaga gatttcgtca
      901 cttgataatt cagcaatctc atgaaactct gagttgggat ttagattctt ttcttctgcc
      961 ataacatata aataagctaa tgaaaaagcg ttgtctcttt gatcgcgtag actgttttca
     1021 attgacgaaa ctacaagacc acgaagtaac catgttttat cgcctgttgc ttgaatattt
     1081 tcggctgcca cttgaacata gccaattaga aaaagatcta acatattagg tgatgcaggg
     1141 cttcataagt tccctaatct cttcgcgttc ttctgatctg gaggttaggt agatctcata
     1201 atgctgattt aagaattcat aaatattatt tttgatgtca ctcattgatt catgggaatc
     1261 ttgaaatcga taaatcctgt ttattcgttg atctaattgt tcgataaagt catttgcatt
     1321 cataaatgtt tacaaatacc aaaacttgga tgtcaagcct actggcttag gacctcaccc
     1381 ccaccatcta cccctttggg gtacatatcc taaaagagag ggagtctgct ggtaagtttg
     1441 atgacgatga gaatgtgggt gaggtattgt cataataaat cttttcaaag ttataaattt
     1501 tcacgccact gctggatttc aagagtatga tgttgatagt cttgcatcag ggattggatc
     1561 actcctgcaa tggttgcagg gcgccctcga tagtgaaata ctccatgact atcccacagg
     1621 tcattcggtg gaagcgacgc aataaatgtt ctaaacgtat tcattgacga ttctaattcg
     1681 gcgagcagtt cttcttttga ctgtgatgaa aaagtaatca ccatttgggc attaatattt
     1741 ctatagtcgt tgagggcatc gtcgtaatag gaaggcgtct gcccattaag aattgaggaa
     1801 gaggcttcga tcatacgctt gttccaacca atcaggtgtg caacgacatc tcggggagac
     1861 cagccattca tctcagataa caataaagaa tctggcagcg agaggataca agtagagaaa
     1921 tcatcgtagg ccaactgcag gtcaggtaaa agattttcag atttcacaga atggtcattc
     1981 attacgtaaa tttagcggtt gtcccttgtt ttctgctatt ttttcagaga gtacctggta
     2041 attttagagg ctggtcactt gcaaaaccta tcttactccc taaattaacg atactcttag
     2101 gatgacactt aattttgtct gggctttttt gcgacaatat tccttaccaa tcacccctcc
     2161 gccatcaccg gatgcatacg cttgattgtc atcgaaaatc cggcggcaat gaaaccgagt
     2221 accgcggcag aaaagaatgc aacagtgtaa tcgcccaaca gatcgcgcag cacaccgcca
     2281 atatatgccg caaccgcggc accaagcata tgcgaaaaga aaatccagcc gtatagcacg
     2341 ccaacggagg cgcgcccgaa tcgttgggcg gtcaggttga tcgtcggggg tacggttgcg
     2401 atccaatcga gcccatacac aaacgcaaaa atgaacaagc cggacatatc gctgatgaat
     2461 gggagaaatg cgatagacgc cgcgcggaat gaatagtaca cggcgagcaa cattcgattg
     2521 tcgtaccgat cactgaacca accagaggca agtgtgccga caatgttcat caatcccatc
     2581 agtacaatgg cattcgcggt agacgcgcta tcgaaaccgt gttcaatcgc atgtggcagg
     2641 agatgcgtgc cgatcaatcc gttcgttgtg taaccgcaca caaaaaaact cgcggccagc
     2701 aaccaaaagt ctgttgtgcg ggcggcctcg gcaagtgagg tttggcgcgc tgcgtccgcg
     2761 acctcagcaa gcgtggtcgg cgtgtcagta tcgcagtacg gattcagacc tttgtcgcgc
     2821 ggaaattccc gcatcaacca gacgaccgga atcaccactc ctgcgacagc gacggcaata
     2881 attaggatcg ctccacgcca accaccccat gttgtcagcg cgatcattgt gggcaaaaaa
     2941 atcagttgcc cggcagcaga tgctgcgccc atcaccccga cgatcaggcc acgatgttgg
     3001 ttgaaccagc gttgcgcgac aatcgccccc aaaacattgg ccagcgcgcc tgtgccgatc
     3061 ccaacgagca cgccccagaa caaatgcaat tgccagagcg tgttgatctt caacatcgca
     3121 ctcagtccca tagcgacaag aataagaccg cccgtcatca tgcggcgcaa tccaaagcga
     3181 ttcaccaata ttccagcgag cggtccaccg agaccaaacc acaaaatgct taccgcaatt
     3241 gcgaacgaaa tatttgcgcg tgtccaacca aactcttgtt cgagcggttt gatcaaaacc
     3301 gggggtgcag ttcgcacgcc accagcaacg agcaatacca ggaaggtaat cgcgacgaca
     3361 atccatgcat aatgcagttt acgatgagtc atacagattc ctttacatat tccagcttaa
     3421 ccaaaaatgc cctcataggt gatggactat catcgagtgg ctgagacact tcggcaggct
     3481 cagtgcatcg tctgtcagat cttaaacaaa ccaaaacttt gatgttaatc cagcttgcct
     3541 gaaaacctct gcaacttgct cgaggcttcg cccatgataa tctatggata ggcggttgcc
     3601 ataccataac tttgaaagat cccaaacctg aagaagagtt agcgtttctc ctcgttccag
     3661 gttatttcgt ttgcaccact ga
//
LOCUS       DASTJP010000640         5629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_694931, whole genome shotgun sequence.
ACCESSION   DASTJP010000640 DASTJP010000000
VERSION     DASTJP010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694931"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VFQ23_18775"
     CDS             complement(<1..968)
                     /locus_tag="VFQ23_18775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9908700.1"
                     /translation="MGDYRLKILAEARGFLEKQGGSESDREILLKLLEILTAALERYS
                     PEDHSNTQGIARELINHRALLAMLKQQTDELDALKKLSINLTSSLDLSDVLDAVVSEA
                     VRLIENTRDVNIFLYKNYRLSFGASLDTEGIRNKPFSKPRSNGVTYTVARKGETMIVE
                     NIRTHPLFTTAPKDWIGSIISIPLKVGDTVLGVMNLSRSTTGGFSSSELRLLTLLSDQ
                     AAVAISNASLHQMISRQAYSDTLTGLPNRRALDERLEEEVLSARRNNYSFAVIMMDLD
                     GFKDVNDTYGHSIGDDVLRLVFNQMARGVRNTDFLARYGGDELTLIL"
     gene            1296..2087
                     /locus_tag="VFQ23_18780"
     CDS             1296..2087
                     /locus_tag="VFQ23_18780"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9908701.1"
                     /translation="MSELNKPASATPLHPRRRGIIIGASDGLGAELARLLAREGYTLG
                     LIARRKELLESLRDEINSASNEQRTFAYVHDVSDYEKAPELLRKIVSELGGLDLVIFL
                     AGVNYPPGGIDKYNFENDRKMIEVNLIGAMAWLHPIAEMFQSAKAGQIVGIGSVAGDR
                     GRVGNPGYNTSKAGLATYLEALRNRLTRHGVNVLTVKPGFLKTEMLKAAQGPTPFAIP
                     PQKAAEDILKAIKARKQVIYTASIWRWIMLAIQHTPSFIFRRLSF"
     gene            2126..3532
                     /locus_tag="VFQ23_18785"
     CDS             2126..3532
                     /locus_tag="VFQ23_18785"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9908702.1"
                     /translation="MQPKLHSHFRQLENFGHSLKASSYQIAIKKTDDIFEAFQLAKKL
                     GLKVVARGTGISYNDASLNGGGIVLDMSGMNQILEWDSSSGVIRCEAGVTLRELWRCV
                     EPDGWWPPVVSGTMRTTLGGCLSTNIHGKNNFRVGPIGEHVVEFTAILPTGEEITCTP
                     DKNADMFYAMISGLGMLGIFTSITLKMKRIHSGLISVDAWHVPHLNRHLSDIYENAPQ
                     YDYIVGWFDTFARGKSLGRGQIHGARQLKEGEDPNSRETLQLKYQHLPDRFFGVIPRS
                     LLHYFMNPMMNDFAWRGVNTGRYISALRKHTFRESHAEFHFLLDFVPNWERSFGRGGL
                     IQYQSFLPKETAEDAWREMISLSHQRGLPAYLGVTKRHRPDKFLLTHAVDGFSLALDF
                     KVTDKNRAALRNMLHDFDQIVIKAGGRFYFAKNSETSAEVTRKFLGEETIARFKELKK
                     DSDPDNLLESDLFRRVFG"
     gene            3529..4962
                     /gene="sthA"
                     /locus_tag="VFQ23_18790"
     CDS             3529..4962
                     /gene="sthA"
                     /locus_tag="VFQ23_18790"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HET9908703.1"
                     /translation="MKDQPEKYDLIVIGGGPAGEKGASKAAYYGKHVALVEREPYLGG
                     AGINTGTVPSKTLRESALYYSGLQQRGLYGIDYSFKKNLSIKNFMHRERIVVDNERQI
                     IEKTIKRHNITVIQGEGSLKDSHTVHVKSPNSEIDLSGEIILIATGSSPRRPAEIPFD
                     GKLIFDSDTILDMNRIPRTMVVVGGGVIGTEYASIFTALGIQVTLIEPRDRIVPFLDS
                     EIATRLMDQLTKLGLKFIFNDRMTSIEPKADHVQVTLEKGGIMDFEVALVAAGRQSNV
                     EGLGLEQVGVKTGERGLILVDENFQTNIPNIFAVGDVIGFPALASTSMEQARAAIVHA
                     FHLEYKEKLAPFLPLAIYAIPEVSSVGLTEDECKAKNTPYLIGRAYYEENARGQIIGD
                     LGGMIKLVFSPEDKKLLGAHIIGEQASELIHMASHVMLSHGAIDAFIEAVYNYPTLSD
                     SYQYAAYDGLKNHDIWLESTKNGIRSS"
     gene            4943..>5629
                     /locus_tag="VFQ23_18795"
     CDS             4943..>5629
                     /locus_tag="VFQ23_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase"
                     /protein_id="HET9908704.1"
                     /translation="MVYEVLDLGLIEYEQAWKLQDQYAAEIAEGKRPPTLLLLEHPHV
                     YTFGRRGKQENLLWGESQLKEKGITIHWVDRGGDVTYHGPGQLVGYPLLPLRPVRSSE
                     DAERQGVLAAHTAESTSNTLRPDRSDARIPQADYVGYVRNLEKTLITALAQLGLPAGQ
                     RSGLTGVWIQADVHSRCPRCKPEDRKKPAKIAAIGVKVDARGVSRHGFALNVDPEMDY
                     WHGIIACGLQN"
BASE COUNT         1462 a         1376 c         1422 g         1369 t
ORIGIN      
        1 aggatcaggg ttaattcgtc accgccatac ctcgccagaa agtctgtatt acggacgccg
       61 cgcgccatct gattgaatac caatcgcaaa acatcatcgc ctatggaatg cccataggtg
      121 tcattcacat ctttaaatcc atccaggtcc atcatgatca ccgcaaagga ataattattc
      181 ctgcgcgcgg ataaaacttc ctcttcaagt ctctcatcga gcgcgcggcg attgggaaga
      241 cctgtcaacg tgtcgctgta ggcctggcgg ctgatcatct gatgaaggct ggcattggaa
      301 attgccacag cagcctgatc ggaaagcaag gtaagcaaac gtaattcaga cgaggaaaat
      361 cccccagtcg tcgaacggga gagattcatc acgcctaata cagtgtcgcc gaccttaagc
      421 ggaatactga taatcgagcc aatccagtcc ttgggtgcag ttgtaaatag aggatgggta
      481 cgaatattct cgacaatcat ggtttcccct ttccgtgcaa cagtatacgt aacaccattc
      541 gagcgaggtt tcgagaatgg cttgtttcgt atcccttctg tatccagtga cgccccgaaa
      601 gaaagacggt aattcttata cagaaagata ttgacatcgc gcgtgttttc aatgaggcga
      661 acggcttctg agaccactgc atcgaggaca tcggacaagt ccaggctgga cgtcaggttg
      721 atgcttagct ttttgagggc atccaattca tcggtctgct gcttgagcat ggcaagcaat
      781 gccctatggt tgatcaactc acgggcgatc ccttgggtat ttgaatggtc ttcgggggaa
      841 tatctttcga gtgcggcggt taaaatctcg agcaatttga gaagtatttc cctgtcgctt
      901 tcggaaccac cctgtttttc cagaaagcca cgggcttctg ccaatatttt taatctgtag
      961 tcacccattc ttgttgtttc tcagcaagac cgacttgttg aaataaaacc tgagagagtc
     1021 ctttctaact gtcctaaatt gccgctcgtc attaagcaag aaaactttct caactggttt
     1081 gaaagccaca cttaactcgg aagcttactt acgcatgtct gacatgcgtg cggcactctg
     1141 taagtagcga cattataccg acttgcctgc agttagtgta ttacatacgc gttacaacca
     1201 ccatcccggt cgagttctat tatagaatag gtttctgttg ctcaggatat ggttggtggg
     1261 tatgttagaa tttgctcacc tatttggagg atactttgtc agaattaaac aagccagctt
     1321 ccgctacccc attacatccc cgccgacgcg ggatcatcat cggcgcgtcg gacggactcg
     1381 gcgctgaatt ggcgcgtctc cttgctcggg aaggctacac gcttggcttg atcgcccgca
     1441 gaaaagaact tctggaatcg ctacgagatg agattaatag tgcatccaac gaacagcgca
     1501 ccttcgcata cgtccatgat gtatccgact acgaaaaggc acccgaactc ttacgtaaga
     1561 tcgtttctga gcttggcggc ttggacctcg ttattttcct ggcaggtgtg aattatccac
     1621 cgggcggaat tgataagtac aatttcgaaa atgatcgcaa gatgatcgag gtaaatctca
     1681 tcggggccat ggcctggctg cacccgatcg ctgaaatgtt ccagtccgca aaagcaggtc
     1741 aaattgtggg tattggatcc gtcgcgggtg accgcggacg agtaggcaac ccggggtaca
     1801 acacttccaa ggcagggctg gccacgtatc ttgaagctct gcgtaaccga ctcacacgtc
     1861 atggggtgaa cgtgctgacc gtcaagcctg gttttctaaa aacagaaatg ctcaaagctg
     1921 cacagggtcc gacgccgttt gcgattcctc cgcagaaagc ggctgaagat attttgaaag
     1981 cgatcaaggc gcgtaaacag gtaatctaca ccgcttccat ctggcgatgg atcatgcttg
     2041 ccattcaaca cacgccatcc ttcattttca gaagactatc gttctaatcg acacagcaat
     2101 gattcgcgtc gaaccttcat tcatcatgca acctaaactc catagtcact tcaggcaact
     2161 cgaaaacttc gggcattctc taaaggcctc ttcttatcag atcgcaatca agaaaaccga
     2221 cgatatattc gaagcatttc aactggcaaa aaaactcgga cttaaggttg tggcccgcgg
     2281 aacaggcatt agctataacg acgcctcgct caacggcggt ggtatcgtcc tggatatgtc
     2341 tgggatgaat cagatcctcg aatgggattc gagctcaggc gtaattcgat gtgaagcggg
     2401 agtcacattg cgggagctct ggcgctgcgt tgagccagat ggctggtggc cgccggtcgt
     2461 ctcaggcacg atgcgcacca cgcttggagg atgtctctcc acgaatatcc atggcaaaaa
     2521 taattttcga gtaggaccaa tcggcgaaca cgtggtggag ttcactgcca tcttgccgac
     2581 tggcgaagaa atcacatgca cacccgataa aaacgcagat atgttctacg ccatgatcag
     2641 tgggcttgga atgctgggca tattcacctc catcacacta aagatgaaac gcatccattc
     2701 agggcttatc tctgtagatg catggcatgt cccccacctc aaccgacact taagcgacat
     2761 ttacgagaat gctccccaat atgattacat tgttggctgg ttcgatacgt tcgcacgcgg
     2821 aaagagtttg ggacgcggac aaatccacgg cgcgcggcaa ctgaaagagg gtgaagatcc
     2881 aaactcgcgc gagacgcttc aacttaagta tcagcatctg ccggatcggt tctttggtgt
     2941 gataccaagg tccttattac attacttcat gaatccgatg atgaacgatt ttgcctggcg
     3001 gggtgtaaac acgggcagat atatctctgc gctgcgtaaa catacattcc gcgaatccca
     3061 tgcagagttt cactttttgc ttgactttgt cccgaattgg gagcgctcct tcgggcgcgg
     3121 tggattgatt cagtatcaat ccttcctgcc gaaggaaacc gctgaagatg cctggcgcga
     3181 aatgatcagt ctttcacatc agcgcggctt gccggcttac ctgggagtga caaaacgaca
     3241 ccgcccagat aaattcttat tgacccacgc cgtggatggc ttctctctcg cattggattt
     3301 taaagtgaca gacaagaacc gcgcggcatt acgcaacatg ctacatgatt ttgaccagat
     3361 cgtcatcaaa gctggtgggc gtttttattt tgccaagaat agtgagacat ccgcagaggt
     3421 cacgcgcaaa tttcttggtg aggaaaccat tgcaagattc aaagaactaa aaaaggatag
     3481 tgaccctgac aatttgctcg aatcggattt gtttcgaaga gtatttggat gaaggaccag
     3541 ccagaaaaat atgacttgat cgtcattggc ggcggaccgg ccggggagaa gggcgcctcg
     3601 aaagctgctt attatgggaa acatgtggca ctggtcgagc gtgaacccta tcttggtggc
     3661 gctggaatca acactggaac ggtcccctcg aagaccctgc gtgaatccgc tctgtattac
     3721 tccgggttgc agcagcgcgg cttatatgga atcgactatt cctttaagaa aaatctatcc
     3781 atcaagaact ttatgcatcg tgaacgaatc gtagtggaca acgaaagaca aatcatcgag
     3841 aaaaccatca agcgccataa tatcaccgtc atccaggggg aaggctcgct aaaagattca
     3901 catacggtgc acgtaaaatc gccaaatagt gaaatcgatc tctcaggaga aattatcctg
     3961 attgccactg gatcctctcc gcgtcgtcca gctgaaatcc cattcgatgg aaaattgatc
     4021 ttcgattcag ataccattct ggatatgaac cgcatccccc ggacaatggt ggttgtgggc
     4081 ggcggcgtga tcggcacgga atatgcctcc atttttacgg cgcttggcat ccaggtcacg
     4141 ttaatcgagc cccgtgatcg tattgttcca tttctagatt ccgagatcgc cacacgactg
     4201 atggatcaac tcacaaagct tggacttaaa tttattttca atgaccgcat gacgtccatt
     4261 gaaccgaaag cagatcacgt acaggtgact ttggaaaagg gcggcatcat ggactttgaa
     4321 gttgccctgg tcgcggcagg caggcaaagc aatgtggaag gattgggact ggaacaggtc
     4381 ggcgtaaaaa caggagagcg cggcttgatc ctggtggacg aaaacttcca gaccaatatt
     4441 cccaatattt tcgcagtggg tgatgtgatc ggttttccgg cgcttgcatc cacctccatg
     4501 gagcaggcgc gggcggcaat tgtgcatgcc ttccatctgg aatataagga gaagctggcg
     4561 ccgttcctgc cgttagctat ctatgcaatc ccggaggtat cctcggttgg tctaactgag
     4621 gatgagtgta aggcaaagaa tactccatac ctcattgggc gcgcttacta tgaagaaaac
     4681 gcacgcggtc agatcattgg tgacctgggc gggatgatta aactggtctt ctcacccgaa
     4741 gataaaaaat tacttggtgc gcatattatt ggagaacagg cttctgagtt aatccacatg
     4801 gcttcgcatg tgatgttatc gcatggcgca atcgacgcgt tcatcgaagc tgtgtataac
     4861 taccccacgc tttcggattc ctatcaatat gctgcctatg atggactgaa aaatcatgat
     4921 atatggcttg agtcaacaaa gaatggtata cgaagttctt gatttgggtt taatcgaata
     4981 cgaacaggcc tggaaattac aagaccaata cgcagcagaa atcgcagagg gaaaacgtcc
     5041 gccgactttg ctgttgctcg aacatcctca cgtctacacc tttggacggc gtggcaaaca
     5101 ggaaaacctg ctgtggggcg aatcacaact caaagaaaaa ggaattacga ttcactgggt
     5161 ggaccgtgga ggtgatgtca cctatcacgg accggggcag ttggtagggt atccgttgtt
     5221 gccattaaga cctgtcaggt cttcggagga tgccgaacgt caaggtgttt tggccgcgca
     5281 tacagccgag tcgacgtcga acacgctgag acctgacagg tctgacgcgc gcattcctca
     5341 agcagattac gtcggctatg taaggaattt ggaaaaaaca ctcatcacag cattagcaca
     5401 gctaggtctc ccggctggac agcgatctgg tttgacgggt gtgtggattc aggcggatgt
     5461 gcattcgcgt tgcccgcgct gcaaacccga ggatcgcaaa aagccagcca agatcgcggc
     5521 catcggcgtc aaagtggatg cccgcggtgt gtcacgtcac ggctttgcct tgaatgtgga
     5581 tcctgaaatg gactactggc atggcattat cgcctgcggg ttgcagaac
//
LOCUS       DASTJP010000641         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_531425, whole genome shotgun sequence.
ACCESSION   DASTJP010000641 DASTJP010000000
VERSION     DASTJP010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531425"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..409
                     /locus_tag="VFQ23_18800"
     CDS             167..409
                     /locus_tag="VFQ23_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908705.1"
                     /translation="MSLDDAIQQMTGTIKAASASAEIKTVKMSDEEARLSVYAPAGDM
                     QKIKDATFLPAIELLNSDGMDIQVFVYDKDAPPLKG"
     gene            complement(487..1452)
                     /locus_tag="VFQ23_18805"
     CDS             complement(487..1452)
                     /locus_tag="VFQ23_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908706.1"
                     /translation="MAFRLNRNLILSLMICSLLTACGAESTPAPTQIPSSPTSNFFEM
                     PVTTPLPSATAKPRQVSPITPVPTRTITPVPIPEGTASSAWKSWPVIPESVDPSLQRV
                     YERGLALGNDPNAFSIFGDCQARPGEFFGLFETDEEVFESLAPDLQETVSHFMGSFNR
                     ESSTAQDGTTPGSLLWIQWHRGEYSCTFSETPVECELRTHRPSFVIIQIGTHFESRNT
                     EYLRRIILQLMDAGVVPILATKADNREKDERINRDMALLAVEYDLPLWNFWAAVSDLP
                     NRGLYTRDDRPLQGDIYLTEEAAVIHRMTGLEVLDVVWRAVAGEN"
     gene            1537..>2045
                     /locus_tag="VFQ23_18810"
     CDS             1537..>2045
                     /locus_tag="VFQ23_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908707.1"
                     /translation="MTINTIHEIGTVIRNAVIFNFKNSSEVLGKAESLVAQTEEFFKL
                     LNARDIDFVLVNGIALLTYVEGRNTQDIDLIMTLPSLSKLPEIKLENEDKDFARGKFG
                     ELQIDILLTKNLLFAKVEKNYTTTKQFMDRTIRCATVEGLLLLKLFALPSLYRQGSFE
                     RVGIYENDIA"
BASE COUNT          531 a          471 c          538 g          505 t
ORIGIN      
        1 ttgaggagcc gaaggagaac gaaggtggcg cgggcgtgac cgttgcgaag atcgcgaaat
       61 aacatcgtgc cctttgcgta gcggagcccg atggttgagt agcgaacgga gtgagcgtat
      121 cgaaactatc ggggtgtagt cgaagcgcag cttttaggag taattcatgt cacttgacga
      181 tgccatccaa caaatgacag gtacgatcaa agccgcgtcc gcgagcgcgg agatcaaaac
      241 agtgaaaatg tctgatgaag aggcacggtt gagcgtctat gcaccggcgg gagatatgca
      301 aaagatcaag gacgccacgt ttcttcccgc gattgaattg ttgaacagtg atgggatgga
      361 tattcaggtg tttgtctacg acaaagacgc gccaccgttg aaaggctaat tttgtaaggg
      421 cgtggtcctc acgcccagat tatgttccaa taatattcgg gcgacgtcgc gtcgccccta
      481 cgttttttaa ttttcccccg ccaccgcgcg ccaaacaaca tctaacactt ccagcccggt
      541 catccggtga atgacagccg cttcttctgt caaataaata tcaccttgca gagggcgatc
      601 atcgcgtgtg tataagccgc gattgggcag gtcggagacc gctgcccaga agttccagag
      661 cgggagatca tattcaacag cgagtagcgc catatcgcgg ttgatgcgct cgtccttttc
      721 gcggttatcg gctttcgtgg caaggatcgg caccacaccc gcatccatca actgcaggat
      781 gatccttcgc aggtattccg tgttgcgaga ctcaaagtgt gtgccgattt gaataatgac
      841 aaacgagggg cgatgcgtgc gcagttcgca ttccacaggc gtctcagaaa acgtacagct
      901 gtactcgccg cgatgccatt gaatccataa gagcgagccg ggagtcgttc catcctgcgc
      961 ggtggaactt tcgcgattga atgagcccat aaagtgagac acagtctcct gtaagtcggg
     1021 cgctaggctt tcgaatactt cttcatctgt ttcaaacagg ccgaagaatt caccgggtcg
     1081 cgcctggcaa tccccgaaga tcgaaaaggc attcgggtca ttcccaagtg ccagcccacg
     1141 ttcatagact ctttgcaggc tgggatctac agattccggg atcacaggcc agcttttcca
     1201 ggcggaagat gcagtgccct caggaattgg tacaggcgtg attgttcggg ttggaactgg
     1261 cgtgatcggg gaaacttgac gcggtttggc agttgcgctg ggcagcggcg tggtgaccgg
     1321 catctcgaaa aaattggagg ttggggatga tggaatctgc gtcggcgcgg gcgtggactc
     1381 tgctccgcag gcggtgagca aagaacaaat catcaaggaa agaatcaagt ttcgattcaa
     1441 tcgaaaagcc atggtaaaaa tattatcata gagcggacat ccccacttcg taactaatcc
     1501 ttccctaatg cttttgaaca gtatacttaa cctattgtga ccattaatac aatccatgaa
     1561 attggtactg taattcgtaa cgccgtcatt tttaatttca aaaactcttc tgaagtttta
     1621 ggaaaggcag agtctcttgt cgctcaaaca gaagagtttt tcaaactgct taacgctcgc
     1681 gacattgatt ttgttttggt aaatggcatt gccctgctca cttacgttga aggtcgcaat
     1741 actcaggata ttgatctaat tatgacattg ccttccctat cgaaattacc tgagatcaag
     1801 cttgaaaatg aggataaaga tttcgcaagg ggaaagtttg gtgaattaca gatcgatatc
     1861 ctgctcacaa aaaatctgtt gttcgcgaaa gtggaaaaga actacacaac gacaaagcaa
     1921 ttcatggatc gtaccattcg ttgcgcgact gtagagggac ttcttttgct aaaattattc
     1981 gcgctacctt ctttgtatcg tcagggtagt tttgagcgcg tggggattta tgaaaatgac
     2041 attgc
//
LOCUS       DASTJP010000642         7730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1213980, whole genome shotgun sequence.
ACCESSION   DASTJP010000642 DASTJP010000000
VERSION     DASTJP010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213980"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFQ23_18815"
     CDS             complement(<1..511)
                     /locus_tag="VFQ23_18815"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9908708.1"
                     /translation="MNRRRRRPNYFGWTIFGLVVLFGYFFNQVYLPSQPNPFEATPTV
                     TRSPESYVTEAEALFNDGKLLQAIDAYQAAIKASPQNPVLYIAIARLQVWAGQYEEAE
                     ANAENALLLSSNNSMAHAVHAWALDFQEGKNGEAQASIVKALELDPNNALAHAYYAEI
                     LVDSNLFENY"
     gene            617..1372
                     /gene="trmD"
                     /locus_tag="VFQ23_18820"
     CDS             617..1372
                     /gene="trmD"
                     /locus_tag="VFQ23_18820"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET9908709.1"
                     /translation="MRFDIFTILPEVFPPYLESSMLLRARQRGLIDVRVHNIRDYTHD
                     KHHTTDDTPYGGGGGMVMKPEPVFDAVESVLGIEAVQTLSQPIPVILLTPQGRVFNQS
                     IAEELSHHERIALLSGRYEGVDERIREHLVTDEISVGDYVLTGGELPALLMIDAISRL
                     LPGVLGDPDGAQDDSHSMGLLEYPHFTRPPDFRGWGVPEVLLSGDHGKIERWRREQAL
                     LRTYNKRPDMLEKADLSAADQKFLDGLRKQEKL"
     gene            1474..2742
                     /locus_tag="VFQ23_18825"
     CDS             1474..2742
                     /locus_tag="VFQ23_18825"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9908710.1"
                     /translation="MKFNYGKTFLLGFGFLGISIVWPIFNQFIPIFLQAGNPEFERQL
                     LSEGRAVPDIVGFGLAPSLALFIMTWDNIINMFIQPWVGAKSDQTWNRFGRRKPWILA
                     GAPIAILGFIFIPVAQTVLAMAAFILITNFGMALFRSPTVAWLGDLFEPNDRSKANGI
                     INLMGGVGGLLAYFGGGILFNSFGRVAPFVAGAVVMVVALLIVLIFVREPEQIATEVK
                     EESAGVLDNLKVVFENPDKSGLFVLLGILFWFMAFNALETGLSSFAVFTLGMQAGTAS
                     IYTGLVTITFILFAVPAGFFGTRFGRGPVIRVGLIGLSILALLGYFFIQNTLTFAIVL
                     ILIGSFWAMVNVNSLPLVYDHGDEKRIGAFTGLYYLSSQFAAVLGPTLGGVLVDAMGS
                     QYRWLWLFSAIFMAIALGMMFGVRNRVATA"
     gene            2888..3934
                     /locus_tag="VFQ23_18830"
     CDS             2888..3934
                     /locus_tag="VFQ23_18830"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9908711.1"
                     /translation="MKKMYVLLAALLISAMVLPACAPAASGGNCADAETFCVGLVTDV
                     GKVNDKSFNQSAWEGVQRAQSELGATVQYIETADSKDYEKNIATFADESYDVIVTVGF
                     GMGEATVKSAGLYPDIKFIGVDQFQAEETAGVAGLNFPEDNAGFLVGALAAMMSKSNK
                     IGAVCGTDVVPPVWRFGEGYKAGAAYADGMMGTTTEVFVVYHSDVGFDKTFTDPEWGA
                     QTAQSMMDQGADAIFGCGGITGNGAITAAAQAGAYAIGVDTDQYLTLPEAAPRMLSSA
                     MKLITPGVFDLIKTAKDGTFTSGNVFGDAGYAPFHDLDSEVPAEVKAEMDKINAGLLD
                     GSIKTNVPPVKPEA"
     gene            4072..5199
                     /locus_tag="VFQ23_18835"
     CDS             4072..5199
                     /locus_tag="VFQ23_18835"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908712.1"
                     /translation="MQSQTQSGSPTKSLFRSALDAMLIPFLAILTAVVLGGIIIVLVG
                     GNPFLAYAGLLEGSFGSIKALSETSVWATPYIFAGLAVALAFRGGLFNIGAEGQLALG
                     AVASALIGYALPGWLGFDLPAIIHLPLAVIMGMAAGAIWAAIVGFLKAYTGGHEVINT
                     IMMNYIALNTISFLLNGPLKDRSPNNVIARTPLIEESARMPVIFDGLRVHWGFVLALL
                     AAFVVWWMLNKTTLGFEIRTVGINPDAAKYAGINVKRIIIVTMGLSGMLAGMAGALEV
                     TGLNYRHELGYSVGYGFDAIAIALLGKSHPFGVVLSAILFAAMRNGATRMQFLTQLPI
                     DLISVLQALILLLVAADAIIRYIYRIRAQGERVVLTRGWGG"
     gene            5208..6320
                     /locus_tag="VFQ23_18840"
     CDS             5208..6320
                     /locus_tag="VFQ23_18840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908713.1"
                     /translation="MDLKRFGFIFLIIIVMFGVVVLVGQTKSPPIVIITSMLATTIAV
                     ATPLALGALSGVFCERSGVINIGIEGMMLSSAFFGWLAAIYMFHIFNFGVGVSITIGA
                     LFAVIAGVLMSLILAVLSITYKVDQVIGGTVINILAVGLTGFLNRQLFFGSGSVFGGN
                     VPNSPGVLPTIHVPITEIFSPLCGEAAGCLRAVAAINDVFDQKPIAISAILLVFISHY
                     VLFYTRWGLRTRAVGEHPKAADTVGINVIKMRYANVMIGGALAGLAGAYFTIESVPSF
                     EPLLTNGRGFISLAAVIFGNWTPIGSWIASLVFGASSALNINIQIFRDVIPPQWAFLQ
                     NSYIVGLIPYILTMFILSGIIGRTTPPAADGVPYDK"
     gene            6329..>7730
                     /locus_tag="VFQ23_18845"
     CDS             6329..>7730
                     /locus_tag="VFQ23_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908714.1"
                     /translation="MTQENSPIVLDARGITKKFPGVLANDNVNLDLRKGEIHVLLGEN
                     GAGKSTLMNILYGLYKPDEGEVIVDGKQMQLNSSRDAIHHGIGMVHQHFMLIPVFTVT
                     ENIMLGAETDHRAAPNENPLVKLDRKEVAKKVADLSRQYGLDVNPEAIVGDLPVGLQQ
                     RVEIVKALYRNANILILDEPTAVLTPQEAQDLIQIMHELTDRGVSIIFITHKLKEVLA
                     VADRITVMRAGRVVGTTTPQESNEQKLAEMMVGREVILTVQKGPAKPAEEILQVENLR
                     VRDVRGLEAVSGVSFSVRAGEVLGIAGVQGNGQTELAEALTGLRHIESGHFKLAGTDL
                     TNKAPRLVTEAGLANIPEDRQRHGLVLSYSITDNMVLCDYYKTPFSKRGVIQQNDLDA
                     NAKKLIKEYDVRTPSPFVAAGKLSGGNQQKVIVARELSRPVKLVIASQPTRGLDVGSI
                     EYIHKEIIVMRDRGVAV"
BASE COUNT         1775 a         1927 c         2074 g         1954 t
ORIGIN      
        1 catagttttc gaacaaattg gaatcaacca ggatctccgc ataataggcg tgtgccaatg
       61 cattgtttgg atctagttcc agagccttca caatggaggc ctgagcctcc ccatttttgc
      121 cttcctgaaa atccagggcc cacgcgtgaa cagcatgtgc cattgaatta tttgagctca
      181 acaataaagc attctcagca ttagcctccg cttcctcata ttgacctgcc cagacctgta
      241 aacgggcaat cgcaatatat agcactggat tctgcggaga ggctttgatc gcggcctggt
      301 aggcatcgat ggcctgcaac aacttaccat cgttaaaaag tgcttccgcc tccgtgacgt
      361 aagactcagg ggaacgcgta accgtcggcg ttgcctcaaa cgggttgggc tggctcggca
      421 ggtagacttg attaaagaaa tacccgaaga gcacgaccag gccaaaaatc gtccagccga
      481 aataattcgg gcgtctgcgt cgccggttca tactccaccg gctacttctc ggtaaataca
      541 tggcagtcat attaccatga aagaataaag ggtgaattaa gaagtaaagg agagaggatg
      601 atgatagaat gcctccatgc ggttcgatat ttttacgatt ctgcccgagg ttttcccacc
      661 ttatctcgaa tccagtatgc tattgcgggc acgtcaacgg ggactgatcg atgtgcgcgt
      721 acacaatatc cgagattaca cgcacgacaa gcaccacacc accgatgaca ctccctatgg
      781 tggcgggggt ggaatggtaa tgaaacctga gcccgtattt gacgctgtgg aatccgtttt
      841 aggaattgaa gctgttcaga ctttgtctca gccgattccc gttatcttat tgacccctca
      901 gggacgagtc ttcaaccaaa gcatcgccga agagctatct catcacgaac gaatagcgct
      961 gctgtccggt cgctacgaag gcgtggatga acgcatccgg gagcatctcg tcacagatga
     1021 aatctcagtc ggtgactacg ttctgacagg cggagaactg ccagcactgt taatgatcga
     1081 tgccatttcg cggctgctcc caggcgtgct cggcgacccg gacggtgcac aggatgattc
     1141 ccattccatg ggcttgcttg agtatcccca tttcacgcgc ccacctgatt ttcgcgggtg
     1201 gggtgttcct gaggttttgc tctctggcga tcatggcaag atcgaacgat ggcgcagaga
     1261 gcaagcttta ttgcgcacat ataataaacg cccggacatg ctggaaaaag cagatctaag
     1321 cgctgcagac caaaagtttc ttgatggtct aaggaaacaa gaaaaactat aacagcacat
     1381 cattgcgagt actgagcttg cgtagccagt tggcaaagtc gcgtagcttc cgctggtcgc
     1441 tactcacaat gacaattaaa gaggagagga aagatgaaat tcaattacgg gaaaacattt
     1501 ttgctcggct tcggtttttt ggggatcagt atcgtctggc ccatcttcaa tcagtttatt
     1561 ccaatctttt tgcaggctgg caacccggaa tttgaaagac aactcttaag tgaaggtcgc
     1621 gcggttcccg acattgtcgg tttcggcctg gcgcccagcc tggcattatt catcatgacc
     1681 tgggataaca tcatcaatat gttcatccag ccctgggtag gagcaaaaag cgaccaaacc
     1741 tggaatcggt ttggacgacg caagccatgg atcttagcag gcgcgcccat cgcgatcctt
     1801 ggtttcattt ttattcctgt tgcgcaaaca gttctggcca tggccgcctt catcttaatc
     1861 acgaactttg ggatggcatt attccgctcc ccgaccgttg cctggctggg tgacctgttc
     1921 gagccaaatg atcgcagcaa ggcaaatgga attatcaatt tgatgggcgg tgttggcggg
     1981 ctactggcgt attttggagg aggtatctta ttcaactcat tcggtagggt ggcgcctttt
     2041 gtggctggtg cagttgtaat ggtcgtggca ttgctgattg tgttgatctt tgtcagagag
     2101 cccgagcaaa tagcaacaga ggtcaaagag gaaagcgcgg gagtgctcga taatttgaaa
     2161 gttgttttcg agaatccaga taaaagcgga ttattcgtgc tgctgggtat cctcttttgg
     2221 ttcatggcat tcaatgcgct ggaaaccgga ctctcttctt ttgctgtgtt cacacttggg
     2281 atgcaggccg gaaccgcgtc gatttacaca ggtctggtga caatcacctt cattttattt
     2341 gcagtcccgg ccggattctt cggaacgcgc ttcgggcgtg gaccggtcat tcgagtcggg
     2401 ttgattgggc tttcgatcct tgcgctgctt ggatatttct tcattcaaaa tacgctgaca
     2461 tttgcgattg ttctcatcct gattggttcg ttctgggcga tggtgaatgt caacagtttg
     2521 ccattggtct acgatcatgg tgatgaaaag cgcatcggcg cgtttacagg gctatactat
     2581 ttatcctcac aattcgctgc ggtactcggt ccgacgctcg gcggcgtttt ggtggatgcc
     2641 atgggtagtc aatatcgctg gctatggttg ttttcagcga tctttatggc aattgccctg
     2701 ggaatgatgt tcggcgttcg taatagagtg gcaacagctt aaaaaacgaa agcaggtgca
     2761 gaaacagtat gaatagattt cgctatctta atgcttgtct tgtcgaatgg ttgaagcgca
     2821 agtatacttg cgctgaaaag ggtttgtgtt aaccctgtca ttcatacatc atgaagagga
     2881 gaagaacatg aagaaaatgt acgttttgct cgcggcgctc ctgatcagcg ccatggtctt
     2941 gccggcctgt gcaccggcag catctggtgg caactgcgcc gatgcagaga ctttttgcgt
     3001 gggcctggta actgacgtgg gcaaggtcaa cgataaatcc ttcaaccagt ctgcctggga
     3061 aggtgttcag cgtgcccagt ccgaacttgg agcaactgtt cagtacattg agaccgccga
     3121 ttccaaggat tacgaaaaga acatcgccac attcgccgat gaaagctacg atgtaattgt
     3181 gaccgttggt tttggtatgg gcgaggcgac agtcaagtcc gccggactgt acccagacat
     3241 caagttcatc ggtgtcgatc agttccaggc tgaagaaacc gctggtgttg ctggtttgaa
     3301 cttccccgaa gataacgcag gcttcctcgt cggcgcgcta gccgcgatga tgagcaaaag
     3361 caataagatt ggcgccgtat gcggaaccga tgtggttcca cctgtttggc gctttggtga
     3421 gggctacaaa gccggtgctg catacgccga tggaatgatg ggcaccacca ctgaagtctt
     3481 cgttgtgtat cacagcgatg tcggcttcga caagaccttc accgatcctg aatggggcgc
     3541 acaaactgcc cagtccatga tggatcaggg tgcagatgcg atcttcggtt gcggtggtat
     3601 taccggtaac ggcgccatca cggctgctgc tcaggcgggt gcctatgcta tcggcgtaga
     3661 tactgatcag tatctcactc ttcccgaagc tgccccacgc atgctctcca gcgcaatgaa
     3721 gctcatcact ccgggcgtgt tcgatctgat caagaccgct aaggatggca cattcacgag
     3781 cggcaacgtg ttcggtgatg ccggatatgc accatttcac gaccttgata gtgaagttcc
     3841 tgctgaagtc aaggctgaga tggacaagat caacgccggt ctactcgacg gctcgatcaa
     3901 gaccaatgtt cctcctgtaa agccagaagc gtaagcttca ctctgatata aaaaaaggaa
     3961 agaggcgtgc ctctttcctt tttttttcct cctatcacat gatgagtttc atggacgcat
     4021 aaaatcaggt gatataatcc gtaaaattat cacggctgaa cgggagacct tatgcagtcg
     4081 caaacacaat cgggctcgcc gaccaagagt ttgtttcgat ccgcgctgga tgccatgctc
     4141 attccgtttc tggccatcct gacagcagtt gttttgggag gaattattat tgtccttgta
     4201 ggcgggaatc cttttctagc atacgcgggg ttactcgaag gttcattcgg ttccatcaaa
     4261 gcgctcagtg aaacatcagt ctgggcaaca ccctatattt ttgcagggtt ggctgttgcc
     4321 ctggcgtttc gagggggctt attcaacatc ggtgcagaag ggcaattggc gctgggtgca
     4381 gttgcatcag cactgatcgg ctacgctttg cccggatggc tgggattcga tctgcctgca
     4441 atcattcacc tgccgcttgc tgtgattatg ggcatggcag caggagccat atgggcagcc
     4501 attgttggtt ttctgaaagc ctacaccggg gggcatgaag tcatcaacac gatcatgatg
     4561 aactatatcg cgttgaacac aatttcgttc ttattgaatg gtcccctgaa ggaccgaagt
     4621 ccaaataatg tcattgctcg cacaccgctc atcgaagaaa gcgcgcgcat gccggtgatc
     4681 ttcgacggac tgcgcgtcca ctgggggttt gtgcttgcgt tgctggcggc ttttgtggtc
     4741 tggtggatgc tgaacaagac cacgcttgga tttgaaatac gtaccgttgg cataaacccg
     4801 gacgccgcaa aatacgcggg catcaatgtg aaacgtatca ttattgtcac gatggggctc
     4861 tcgggcatgc tggctggaat ggccggcgcg ctcgaagtca ccgggttgaa ttatcggcac
     4921 gaactcggct attccgttgg ttatggtttc gatgccatcg ccattgccct gttgggcaag
     4981 tcacacccct ttggcgtcgt gctttccgcc attctctttg ccgccatgcg gaatggcgcg
     5041 acgcgcatgc aattcctcac gcaactaccc attgatctga tctccgtgct gcaggcgttg
     5101 atcctgctgc tcgttgcggc agatgcaatc attcgctata tctatcgaat tcgcgcgcag
     5161 ggcgaacgcg ttgtgttaac ccgcggctgg ggcggttagg agcaaccatg gacttgaaac
     5221 gtttcggttt catcttcctc atcattattg tcatgtttgg cgtggtcgtg ctggttggac
     5281 agacgaaatc gccacccatt gttattatca caagcatgct ggcaacaacc atcgccgttg
     5341 caacgccatt agctctgggg gcgttatcgg gtgtgttttg cgagcgctca ggcgttatca
     5401 acatcggtat tgaaggcatg atgctctctt ccgccttctt cggctggctg gccgccattt
     5461 acatgttcca catttttaac tttggagtcg gcgtcagcat tacgattggt gcgttgtttg
     5521 ccgtaatagc gggcgtattg atgtcactta tactcgccgt cctttccatc acatataaag
     5581 tggatcaggt catcggcgga acagtcatca atatcctggc tgtcggtctg acaggcttcc
     5641 tgaaccgcca gttgttcttc ggcagcggca gtgtgtttgg tggcaacgtc ccgaactcac
     5701 caggggtgct ccccaccatc cacgttccaa ttacggaaat cttttcccca ttgtgtggtg
     5761 aggccgctgg ttgtctgcga gcggtcgcgg cgatcaacga cgtgttcgac caaaaaccga
     5821 ttgctatcag tgccatcctg cttgtgttca tttcccacta cgttttgttc tatacccgtt
     5881 gggggctgcg cacacgcgcg gtgggcgaac atccaaaggc tgcagacacc gtcggtatca
     5941 atgtgattaa gatgcgctat gccaacgtca tgatcggtgg cgctttggca ggtttagccg
     6001 gtgcgtactt tacgatcgag tctgttccct cctttgagcc tttgctcacc aacgggcgcg
     6061 ggtttatttc actcgcagcc gttatctttg gaaactggac cccgatcggt tcctggatcg
     6121 cctcgctcgt gttcggtgca tcgtcagcgt tgaatatcaa tatacagatc ttccgcgatg
     6181 tgatcccgcc gcaatgggcg ttcttgcaga actcttatat cgttgggttg atcccctaca
     6241 tcctcaccat gttcatcctc tccggcatca ttggccgcac aacaccaccc gcggcagatg
     6301 gcgtgcctta cgataaatag gcaaacacat gacacaagaa aacagtccaa ttgttctcga
     6361 tgccagaggt attaccaaaa aatttcctgg cgtgctggca aatgacaatg taaatcttga
     6421 cttgcgcaaa ggtgaaattc atgtgctgct cggcgaaaac ggcgctggca aatccaccct
     6481 gatgaatatc ctctacggcc tttataaacc cgatgaaggg gaagtgatcg tggatgggaa
     6541 gcagatgcag ctcaattcgt cgagggatgc gatccatcat ggcatcggta tggttcacca
     6601 gcactttatg ctcatcccgg ttttcacggt gacagaaaac attatgctcg gcgccgagac
     6661 ggaccatcgg gccgccccga atgaaaatcc cctggttaag ctcgatcgta aggaagtcgc
     6721 caaaaaagtt gcggacctct cacgtcaata cgggctggat gttaatcccg aggcaattgt
     6781 cggtgacctg cctgtgggac ttcagcagcg cgtggagatt gtcaaggcgt tatatcgcaa
     6841 tgcaaatatc ctgattctcg atgaaccgac ggcagtcctc accccgcagg aagctcagga
     6901 cctcatccag attatgcacg aattgaccga tcgcggcgtt tcgatcatct tcatcacaca
     6961 taagctcaag gaagtgctgg cagttgcaga ccgaatcaca gtcatgcgtg cgggacgtgt
     7021 tgtcggaacc accacaccac aggaaagcaa tgagcaaaaa cttgccgaga tgatggtagg
     7081 gcgtgaggtg atcctcactg tacagaaggg tccagcgaaa cctgctgaag agattttaca
     7141 ggtagaaaac ctgcgcgtac gtgatgtgcg tgggcttgaa gctgtgagcg gcgtttcatt
     7201 cagcgtgcga gccggcgaag ttctgggaat tgccggtgtg cagggcaatg gacagaccga
     7261 actggcggaa gccctgacag gcttacgcca tatcgaaagc gggcatttta agctggctgg
     7321 caccgacctg acgaacaaag cgccgcggct ggtcactgaa gccgggttgg caaacatccc
     7381 tgaagatcgc cagcggcacg ggctggtgct gtcctattcc attaccgata atatggtgtt
     7441 gtgcgattac tacaagacgc cgtttagcaa gcgcggcgta atccaacaaa atgatttgga
     7501 cgccaatgcg aaaaagttga tcaaggaata tgatgtgcgc accccgtcac cttttgtggc
     7561 ggcagggaag ctctcaggtg ggaatcagca gaaggtgatc gtggcgcggg aattgagtcg
     7621 ccccgtgaaa ctggtgatcg cttcccagcc cacccgcggt ctggacgtcg gttcgattga
     7681 atatatccat aaagagatca ttgtgatgcg cgaccgcggt gttgcggtac
//
LOCUS       DASTJP010000643         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1133596, whole genome shotgun sequence.
ACCESSION   DASTJP010000643 DASTJP010000000
VERSION     DASTJP010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133596"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFQ23_18850"
     CDS             complement(<1..452)
                     /locus_tag="VFQ23_18850"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET9908715.1"
                     /translation="MKPESIRIAGAGLSGLSAAISLAQRGYRVEVLEKNADSGQLRHT
                     DWDAIENWTTEEDLLACLRQWGIQPTFEHRAPLSFEVYDSKGDCHQVTLPQPFFYLLK
                     RGVERGSLEQALKAQALESGVNIRYNQPASKDNVHIWAAGARSAGFFLG"
     gene            complement(498..1307)
                     /locus_tag="VFQ23_18855"
     CDS             complement(498..1307)
                     /locus_tag="VFQ23_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908716.1"
                     /translation="MLSNTSRSITYILAVLYAILGASLFALPVQLAPVFAWKVTPFMT
                     MTIGAWCLGNAWLAFVVARRWKWSLVYPSLYYLWIFGVGELLILFLFRANLVLVHPIA
                     WLYFITLLVNVIAAVVGIVELVRSRPATLSGLKPTRLQYAGLVAFIVFVGLLGLYGWI
                     AQIGDAGTNGGIFPEIMSLFTLRSFGMFYLSLALAVIPLLWNSNLHTWVSHGFAAFGL
                     ILFITVAAFVYLHLFDFAAHPGGLAYFGAYLAVGIPLLIIFFTFGTGIEKS"
     gene            complement(1473..2312)
                     /locus_tag="VFQ23_18860"
     CDS             complement(1473..2312)
                     /locus_tag="VFQ23_18860"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HET9908717.1"
                     /translation="MKFTDDIKIPDQPYPELRNVLSVFVDEIAAELGDDLVGIYLVGS
                     IASGDFDADSDVDFLVVTNREITEVDMKRLQEIQVKIHAMDCYPAKHLEGSYISIADL
                     NDSSIVGKKKLYYFDNGSTTYEESTHDNQWHVRWILRERGITLIGQKPETILQAIPQT
                     ELLNEIKSAMLQVRRLFEAEIDRPLSFFNSKFGQSFVVLTYCRMLHTLHTGTVQSKKA
                     AATWAKEIVNPRWVNLIDRAWNEREGVRFGAKISQRAEAALLEETREFINYANSQIEI
                     RTN"
     gene            complement(2382..3059)
                     /locus_tag="VFQ23_18865"
     CDS             complement(2382..3059)
                     /locus_tag="VFQ23_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908718.1"
                     /translation="MPADKIVRIGAFASILSGVTWLLLTPFMATIGICQGACLYWQSQ
                     PLIVRTLGRAVANQGWLSFAEPETLYFSYGRYFFLVYVFMMLGLFALHRAHSQRVIQP
                     HQLDRRAYIVLLSSLIIAAVGDFISYGIGVVSQAAWRGGFGLEAIAWLGIMLGAFLYG
                     LVILRHQVFHPVIGLLLILVTVLLPVTFFDRSLIQYAPNAQLLPFAVVWPILGMYLLF
                     GKSRKSV"
     gene            complement(3065..3205)
                     /locus_tag="VFQ23_18870"
     CDS             complement(3065..3205)
                     /locus_tag="VFQ23_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908719.1"
                     /translation="MRFQKAKMGTDSFLTFSGFEHFLVYWQATFPNYTLQAGLIIGNG
                     VY"
     gene            complement(3232..3855)
                     /locus_tag="VFQ23_18875"
     CDS             complement(3232..3855)
                     /locus_tag="VFQ23_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908720.1"
                     /translation="MHSKQNPGYSWKVLLIGGSSGVGKTVAARELAKRLSLSLLLLDD
                     VRLALQHATSRTTHPDLHVFLEYKREQWRDSESIVRDWIAVGNVMSNPLQASINHHIL
                     VPDIGSIIIEGDSVLPIQRNAHEASKDVCTVFILEEEETRLLYNLQSRGRGFNELAQA
                     EQEGFAHASWSYGQWISQQAKRFELPVINARPQRTLPERLLAVTGVE"
BASE COUNT         1136 a         1002 c          977 g          956 t
ORIGIN      
        1 cccaggaaaa aaccagccga gcgcgcgccc gccgcccaga tatgtacgtt atcttttgaa
       61 gctggctggt tatagcgaat gttgacacca ctctccagcg cctgcgcttt tagtgcttgt
      121 tcaagagagc cacgttccac gccgcgtttg agcaagtaga aaaaaggttg cggcagagtg
      181 acttgatgac aatccccttt cgagtcgtac acttcaaagg acagcggcgc gcggtgctca
      241 aacgttggtt gaatccccca ctggcgaaga caagccaata agtcctcttc tgtggtccaa
      301 ttctcaattg catcccagtc cgtatgacgc aattgaccgc tgtctgcatt tttctcaagc
      361 acttccacgc ggtatccgcg ttgcgccaga ctgatcgctg cgcttaaccc ggagagcccc
      421 gcgccagcaa ttcgaataga ctcaggtttc atctgagaga ggaagttgat tctggttcag
      481 acacttgacc aattctctta ggatttttcg atccctgtgc caaacgtgaa gaaaataata
      541 agtagcggaa tcccaaccgc gagataggct ccgaaatacg ccaaaccacc cggatgagcg
      601 gcaaagtcaa acaaatggag ataaacaaat gccgcgaccg ttatgaagag gatcaatcca
      661 aacgctgcaa aaccatgact aacccaggtg tgcaaattgc tattccataa aagtgggatc
      721 acggcgagtg cgagcgaaag atagaacatg ccaaaactgc gcagcgtgaa taatgacatg
      781 atctccggga agatgcctcc gttggtgcca gcgtcaccga tctgtgcgat ccagccgtat
      841 aaccccaaaa gcccaacgaa gacgataaag gcaaccagac ctgcgtattg cagcctggta
      901 ggtttcagcc ctgataaagt tgcagggcgg gaacgcacca gttcaacgat gcccaccaca
      961 gcagcaatca cattgaccag cagcgtaatg aaatatagcc atgcgatcgg atgaaccaat
     1021 accagattgg cgcggaatag aaatagaatc agcaattcgc ccacgccaaa gatccacaaa
     1081 taatataacg aagggtaaac gagtgaccac ttccagcggc gagccacgac gaaggccagc
     1141 caggcatttc ccaaacacca cgcgccaatc gtcatggtca taaaaggagt taccttccat
     1201 gcaaagaccg gcgcaagttg tacaggaagg gcaaagagag atgctcccaa gattgcgtaa
     1261 aggactgcga ggatataagt aatggagcgc gatgtattgg acagcatggt tcctccgaat
     1321 aaatttttgt gattgtaccg tgattaaaag gaacgggcaa gcaacaacgc atttaacgta
     1381 aacataaacg ttgtatcctt gaatcggtcc gttgctatga gagttgcaac agatgtaatt
     1441 atgattaatg gaacaggtcg gattggatat agctaattcg ttctgatctc aatttgcgaa
     1501 ttagcgtaat ttatgaattc ccgcgtttcc tcgagcaacg ctgcctcggc gcgctgactg
     1561 attttggctc caaaccgaac gccttcccgt tcattccacg cccggtcgat caagtttacc
     1621 cacctgggat ttacaatttc ctttgcccat gtcgctgcag cctttttaga ttgcactgtt
     1681 ccagtatgaa gggtgtgtaa cattcggcaa taggtcaaaa caacaaagga ttgaccaaat
     1741 ttggagttga aaaaactcaa agggcgatca atttcagctt caaataacct tcttacttgc
     1801 aacatcgctg attttatttc gttgagcaat tcagtttgcg ggatggcttg caaaattgtt
     1861 tcgggctttt gaccgatcag agtgataccc cgctcgcgaa gaatccaacg aacatgccac
     1921 tggttatcgt gcgttgattc ttcataggta gtgctgccgt tatcaaaata atagagcttc
     1981 ttttttccaa cgatgctaga atcgttgagg tcagcgatgc ttatgtagga cccttccagg
     2041 tgtttggcgg gatagcagtc catggcatga atttttactt ggatctcttg aagccgtttc
     2101 atatctactt cggttatttc ccggtttgtc acaactaaaa agtccacatc gctatctgca
     2161 tcaaagtcac cggacgcaat cgagccaacc aggtagatcc caacgagatc gtctcccaac
     2221 tctgccgcga tttcatccac gaagacacta agtacgtttc gcagttcagg gtaaggctga
     2281 tcgggtattt ttatgtcatc agtgaatttc ataggagcat ctttcaattc ggtcggaagg
     2341 acaaaccggt aggctgacca gatagatttt ctatccgcag atcagacgga tttgcgagac
     2401 ttgccaaaaa gtaagtacat acccaaaatc ggccagacta cagcaaaggg cagtaactgt
     2461 gcattcggtg catactgaat aagggaacgg tcaaagaacg tgaccggcag aagcactgtt
     2521 acgagaatca gcagcaaacc tataacggga tggaatacct gatggcgcag gataacgagt
     2581 ccatacaaaa atgcgccaag catgatgcca agccaggcga ttgcttcaag cccgaaaccc
     2641 ccccgccatg cggcctggga gactactccg atcccatagg aaataaaatc gccaacggct
     2701 gctatgatga gtgatgagag cagcacaatg taggcgcgcc ggtcgagctg gtggggttgg
     2761 atgacgcgct ggctgtgagc gcgatgaagc gcaaaaaggc cgagcatcat gaacacatag
     2821 acaaggaaaa aatagcgccc ataactgaag tatagtgttt cgggttcagc gaacgacaac
     2881 cagccttgat tagcaacggc acggccgagc gtccgaacga tgagtggttg gctttgccag
     2941 tacaagcagg ctccctgaca aatcccaatt gttgccatga aaggggttag caggagccag
     3001 gtaactccgc taaggatgga agcaaatgca ccgattcgta caatcttgtc agctggcaca
     3061 gtgtctaata gactccatta ccaatgatca ggcccgcctg taaagtgtaa ttggggaacg
     3121 tggcttgcca ataaaccaga aaatgctcaa agccggaaaa tgtcaaaaac gagtctgtgc
     3181 ccatcttagc tttctggaat ctcattcaca ccttgttagc cgggttttgc cttattctac
     3241 tcctgtgacg gcaagcaacc gttccggcag ggttcgttgg ggtcgtgcgt taattacagg
     3301 cagttcgaaa cgtttcgctt gctgcgagat ccattgacca tatgaccaac tggcatgggc
     3361 aaatccttcc tgttccgcct gagctaattc attgaatcca cgcccacgag attgcaggtt
     3421 atagagtagc ctcgtttcct cctcttcaag gataaaaacc gtgcagacat ctttcgacgc
     3481 ttcatgcgca tttctttgga ttggaagaac actatcccct tcaataatga ttgaacctat
     3541 atccgggacc aggatatgat ggttgatact agcctgcagt ggatttgaca ttacgttgcc
     3601 aaccgcgatc caatctctaa cgatcgattc ggaatctctc cactgctccc tcttgtactc
     3661 gagaaacaca tgtaagtccg gatgagttgt acggcttgtt gcgtgttgca acgcgagcct
     3721 tacgtcatcc agcaatagca aggataatga caatcgtttt gcaagttcac gggcagctac
     3781 cgttttgcca acgccggaac tgccgccgat cagcagaact ttccaactgt agcccggatt
     3841 ttgctttgaa tgcatgctct cgttttttag cgctgaatag tactgcccca acggtgtgcg
     3901 tccccgcagt ggacccgatc gtgttctatc cagaggaggt agagacaact aagtaagttg
     3961 agaattgtca tcgaagcgga aataaacgca acgcgctgtg gaaacctgca gcaattgata
     4021 cgcatagggg taagtaaaga gacttcaaac cgctttaaat atcacgcgat t
//
LOCUS       DASTJP010000644         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_791877, whole genome shotgun sequence.
ACCESSION   DASTJP010000644 DASTJP010000000
VERSION     DASTJP010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791877"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFQ23_18880"
     CDS             complement(<1..650)
                     /locus_tag="VFQ23_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908721.1"
                     /translation="MNSIRWRLTLSYAAIALLAAFLLGLFLRSVLRTYYDEQEARYLE
                     ANATRIGFIASRLLEVSIPRQIVQDQSISWSFFLQARVRVMDVNGNLIADSGVPQAQQ
                     TLFIKSAEPFEMPVGQAFTAPVNSSEFFQVQILRDEEISTIANAGEDVIVFSPQSVGV
                     ALPVDVSMYGLLDPTSQSLARRSSQVVEAQMSDQAGASLGKVILSDGPAYGDEILN"
     gene            complement(715..1326)
                     /locus_tag="VFQ23_18885"
     CDS             complement(715..1326)
                     /locus_tag="VFQ23_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908722.1"
                     /translation="METQETPAPQKSVLPYVIGVVLIVLLAAAAFIAVRYFRQPKGGP
                     VGLSVPGEQVISFAGPGGGEAQTFALDIEPAKELPATQPDEIGMFVERKDNSVFIGTG
                     EISVIVEAKPGEAPQADSDFSGPKVEVVVSPDTIIYRDTTKLDPENPGATVQQTVEVS
                     TIDEITEQSSISVWGRKAGDRVIADVIVFSTPFMMLAPEASQP"
     gene            complement(1354..>2118)
                     /locus_tag="VFQ23_18890"
     CDS             complement(1354..>2118)
                     /locus_tag="VFQ23_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908723.1"
                     /translation="ASAGGNEEIGHNNVMRWQMDDDLFFVASSSNDKIKAEDIVPNLA
                     RIALGRPYQMPPKTVAVESSILDGYVGQYQLTEGNTLIVSRGNDNELLFTGEGQEAFD
                     LLFQNELGIDVQAAQSAVIKYLNTSSGPQLAKWKTRMTQSLGEFKDLKVIGTAAPLGN
                     GEPWTYVSFEFERGSALTRWIVSPAGALEAALLETDPPYVIFLPERENQFAPFSLSAQ
                     PSVQGIEFSTDTEGNIKMMISVPTGQVDATKIISES"
BASE COUNT          512 a          548 c          520 g          538 t
ORIGIN      
        1 ttgtttaaaa tttcatcacc gtaggcgggt ccatctgaca ggatgacctt accaagtgaa
       61 gcgccagctt gatcgctcat ttgtgcctcc accacttgcg aggagcgacg cgccagtgac
      121 tgtgacgtgg gatccagcag gccatacatg gaaacatcca ccggcagcgc cacacccaca
      181 gactgcggac taaatacaat gacatcctcc ccggcattgg caatggtgct tatttcttcg
      241 tctctcagga tctggacctg aaagaattcg ctcgagttga caggtgcggt gaatgcttgc
      301 cccactggca tttcaaacgg ctccgcactt ttgataaaaa gagtctgctg ggcctgtggc
      361 acgcccgaat ctgcgatgag gttcccattt acatccatca cccgaacgcg tgcctgaagg
      421 aagaaagacc agctgatcga ttggtcctga acgatctgtc gcggtatgct tacctcaagc
      481 agccgtgatg caatgaagcc gatccgcgta gcgtttgcct ccagatagcg tgcttcctgc
      541 tcatcatagt aagtgcgcag aacagagcga aggaaaagcc ccagcagaaa tgctgccagc
      601 aatgcgattg cggcataact aagtgtaaga cgccagcgga tcgagttcat cgggtattgc
      661 atcattattt aaacgaacct gaatatttta caatcgagga gtcaaacggt aatgtcatgg
      721 ttgagaggct tcaggagcga gcatcataaa gggtgtggag aagacaatca catcggcaat
      781 gacgcggtct ccggctttcc ggccccacac cgagatggaa ctttgttcag taatttcgtc
      841 aattgtactc acttccacgg tctgttgtac tgtggcaccc gggttttcag ggtccagctt
      901 ggttgtatcc cgatagataa tcgtatccgg tgagacgacg acttccacct tcggcccact
      961 aaaatcggaa tctgcttgtg gcgcttcacc cggtttcgcc tccacaatca cgctgatttc
     1021 tccggtaccg atgaatacac tgttatcttt tctttccaca aacatgccga tctcatctgg
     1081 ctgggtagcg ggaagttcct tcgccggttc aatatcgaga gcaaaggttt gtgcttcgcc
     1141 gccaccagga ccggcaaatg agatcacttg ttcgccgggt acactcaaac caactggacc
     1201 tccttttggc tgcctgaaat atcgtaccgc aatgaatgcc gcagcggcaa gcagaacaat
     1261 aagcacaacg ccgattacat agggtaagac ggacttttgc ggggcaggtg tttcttgtgt
     1321 ttccatttta tatctccttt agggttattt tctttagctt tcagaaataa ttttggttgc
     1381 atcgacctga ccggtcggaa cagaaatcat cattttgata tttccttcgg tatctgtaga
     1441 aaactcaatg ccttgaacag agggctgcgc gctgagtgaa aagggtgcaa attgattttc
     1501 acgctctggc agaaagatca catagggcgg atctgtttcc agaagggccg cctccagcgc
     1561 gcccgccggg ctgacgatcc agcgtgtcaa cgcgctgcca cgctcgaact caaacgaaac
     1621 gtacgtccag ggctctccat ttccaagggg tgcagcagta ccgatcactt tgaggtcctt
     1681 aaactcaccc agggattggg tcatacgagt cttccacttt gccaattgtg ggcctgagga
     1741 agtgttgaga tacttgatca cagcagattg agcggcttgt acatcaatac caagttcatt
     1801 ttgaaacagc aaatcgaaag cttcctggcc ttcgccagta aataaaagtt cattatcgtt
     1861 gccgcgagag acgatcagtg tattcccttc cgtgagttga tattgcccca cgtatccatc
     1921 cagaatggac gactcaaccg cgactgtttt tggcggcatt tggtatggcc ggcccaaagc
     1981 gattctggcg aggttgggaa cgatatcctc tgctttgatt ttgtcattgc tggagctcgc
     2041 gacaaagaat agatcatcat ccatttgcca gcgcattacg ttgttatgcc caatttcttc
     2101 atttcctccg gcacttgc
//
LOCUS       DASTJP010000645         7233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1426965, whole genome shotgun sequence.
ACCESSION   DASTJP010000645 DASTJP010000000
VERSION     DASTJP010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426965"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFQ23_18895"
     CDS             <1..198
                     /locus_tag="VFQ23_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="catalyzes the opening and hydrolysis of the
                     beta-lactam ring of beta-lactam antibiotics such as
                     penicillins and cephalosporins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET9908724.1"
                     /translation="IGDNFTMGADDALRAVKMLQPKTVIPIHYNTWGLIAQDADAWAE
                     RVQNETSTKVVVLKPGESCSV"
     gene            569..4777
                     /locus_tag="VFQ23_18900"
     CDS             569..4777
                     /locus_tag="VFQ23_18900"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9908725.1"
                     /translation="MNERKTQSPISNLILILAILVSSVLSMRPMGAVKADHTPDPASV
                     NLAGSLESEASGGACGDWDPGCPGAAFTSQGNLVYLFNSASIPSGNYEYKVAMGGWSE
                     NYGSNFQQDGPNINLNLASAQVVRFYYDHKTHYIADNVRNTIYTVPGSFNEELGCAGD
                     WQPECLRTFMSDVDGDGIFTFVTDAIPAGNYEFKIATNESWSNPNYGQGGGPDNIPFT
                     VPGGGATVTFSFNTSNNNPGVAVEPLVVQPGHDNNIFWDGLRHDSRDTLYRTPGGAVP
                     EGTPVIIRFRTFHNDVTSVQLRVFSVNAGGQSLVNMTLAATDVSCYEELDYTCDYWSA
                     TLANANADNLWYRFIVTDGSDTDYYDDNTAALDGGLGATTDEIEDHSWALMVYEPDFT
                     APSWANDAFIYQIFPDRFRNGRSNNDPHTGDVRYDDPVLALPWNTLPEGYCRNYADGD
                     TNCPWRFDTTPPSNSPTKEQPRGRDYMGGDLRGVDQYLTYLKSLGVNTIYFNPIFDAG
                     SNHSYDTQDYTKIDPYFGTQQDWANLVRHGNQLGIRIVLDGVFNHMSSDSPFFDRYHH
                     YPTVGACESVTSEYRSWFNFTDVPAGTGTCVGSDGTANAANYEGWFGFDSIPVLNKTE
                     EEVQAYFLDNPNSIARYWLQQGAGGWRMDVSGDPSFPEGYWENFRSVTKSTKSDALLI
                     SETWQKDSTLLRMLRGDRLDTTMNYRLRDAVIGLLAPGNFDSKGFADSGRIIEPSEFA
                     ARIASMREDYPDAAYYSLMNLLDSHDTERLLWTLTPGAETRAEKEFNTPNLTAGKQSL
                     KIASLIQFTMPGAPTVYYGDEVGVTGDDDPDDRRTYPWADRGGSPDNTLFAHYQTLSS
                     LRTQYPSLTDGDIRILLADDASDTVAYGRKTNAQAAVVVINRGSQSQAVSIPVAGYLP
                     DGTVLTRAYGVGVSGPSSLTVTDGQLTGTIGAKSAWLLVTGTVDLQPTAAPSGLTVSN
                     EGDAEVSLAWNSVAGATGYNVYRSPVTGGGYVKANDTPVTDTTFIDTGLRNAQKYFYV
                     VTALDSAGNESAYSNEVNATPHLVIGWANLQWPPTMTHTISVTDRTDDAYGQVWIDGA
                     TNQPGATESLRAQLGFGSEGTNPATDSSWVWVEATFNVDAGNNDEFKASLLPETTGTF
                     DYVYRYTTTNGQDWLYADLNGPIPQGDVPPNPGKLTVNASGDTTPPAGPTNLDVVSAS
                     PVGIALEWDAVAGDPTLYGYEVLRSASSGGPYDTLALVTGTSYTDTAVAENETYFYVV
                     RAVDTSFNRSDNSNEVEATAELRTVTLVFNVTVPATTDATGRSVYIAGFLDRLDGNNP
                     QWDPGGVVLTRVDATHWTITFTGKESTQLEYKYALGSWDYVEKDVACGEINNRMLTLS
                     YGTTGTQTVEDTVPNWRNVAPCGN"
     gene            complement(4832..5194)
                     /locus_tag="VFQ23_18905"
     CDS             complement(4832..5194)
                     /locus_tag="VFQ23_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908726.1"
                     /translation="MKPGTRLILIYTLVAGVISAITAPIPGTSLLLTALEVFMLVHLS
                     KVNNFQLGLKEIGYSAAAIWGISTALKDVALEILTFFPVIGWIAEVVVAILFVFFLGS
                     LANLYFSNYPSPSANQRK"
     gene            complement(5218..5736)
                     /locus_tag="VFQ23_18910"
     CDS             complement(5218..5736)
                     /locus_tag="VFQ23_18910"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9908727.1"
                     /translation="MEKRVKFDFEIDFTNGGGIQGQDFRLDIEGDDFSDEELADYLVK
                     DMRLLMVGDVRILNKEIIVEKHKRTEDNKPQWLYYLKPTRIEMITEGATEQEQETVSR
                     HFAYVSDLTDKGVMILVGRTQTMDENIFGIAIFEAEDESAARSIMENDPAVAGGVMRA
                     TLYPYKVALMRK"
     gene            complement(5753..6010)
                     /locus_tag="VFQ23_18915"
     CDS             complement(5753..6010)
                     /locus_tag="VFQ23_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908728.1"
                     /translation="MPKIVFQGKTYYSVYEMPPKVRRAYEKEQKQAGTDGKDEGIEPA
                     IQQTSSAFEPDTRAGMRGVMWGILAALILTGIAFLLSRLIP"
     gene            complement(6140..6817)
                     /locus_tag="VFQ23_18920"
     CDS             complement(6140..6817)
                     /locus_tag="VFQ23_18920"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET9908729.1"
                     /translation="MSQSILIVTPLQEEHDDLHSSLASRGLKSHIGKLGRLDVYRFPE
                     LNVTLARGGHGKTQFGIQTQHLLDHAQFDLVICAGAAGALAPEVAVGDIIIATSTLEH
                     DFNNKFSQRPKPSFAGDRKSIEQIKALQLRDADFRIHFGIMAGGDEDVIDVVRGAELR
                     EMTNALAVAWEGVGGARASTFSNIPYVEIRGATDTANHEAPVVFDTNLKIVMKNIAHL
                     LIEWLKP"
BASE COUNT         1747 a         1887 c         1806 g         1793 t
ORIGIN      
        1 atcggtgata atttcacaat gggtgccgat gacgcattgc gcgccgtcaa aatgctacaa
       61 ccaaagaccg ttatccctat tcattacaac acctggggtc tgatcgcgca ggatgcggat
      121 gcctgggcag aacgcgtcca aaacgagacc agcaccaagg tcgtggtgtt gaagccgggg
      181 gagagttgct cagtataggc tcaatacaaa atgaatgccg ggtaatcatc ctggcattta
      241 ttatttccct cccgtaatgc cttcgcctta ggaaatattt gtttcaaaat gttacaaagt
      301 gcatctttga aattgacggt tgattgtctt tgtgctagga tagtatagac atcttaccga
      361 tcatcgaaaa aaattattcg gacaaatcga tgcatgattt cccaggcaat ccaaatcaac
      421 agtcgttttg atagacaaaa aatcatcgcg cttgcgtgat gattttttgt tcgctatttc
      481 tcatgatccc ctcatcgctt tgaaactata aggttccaat ttggaatggc atgttgtaag
      541 accttcatct tattttagga gctgaattat gaacgaacgc aagacacaaa gcccgatttc
      601 gaatctgatc ttgatcttag ccatactcgt gagttctgtt ctatcgatgc gccccatggg
      661 cgctgttaaa gcggatcaca cacctgaccc ggcctctgtc aatctcgccg gaagcctgga
      721 aagtgaagcg agcggcggtg cctgcggtga ttgggatccg ggttgcccgg gagccgcatt
      781 cacctcgcaa ggtaatcttg tatacctgtt taactcggcg tccatcccgt ctggcaacta
      841 tgaatataaa gttgcgatgg gtggctggtc ggagaattat ggctcgaatt tccagcagga
      901 tggtccaaac attaacctga atctggcttc ggcccaggtg gtgcgttttt attacgatca
      961 caagacccac tacattgctg acaatgttcg aaacacgatt tacacagtgc ccggaagctt
     1021 caatgaggag cttggctgcg ctggcgactg gcagccggaa tgtctgcgca ccttcatgag
     1081 tgacgttgat ggcgatggca tattcacctt cgttaccgat gcgattcccg cgggcaatta
     1141 cgaatttaag attgccacca atgagagctg gtccaacccg aactatggac agggtggggg
     1201 tcctgacaat attcccttca ccgtccctgg cggaggagca accgttactt tttccttcaa
     1261 cacatccaat aataatcctg gtgtggcggt cgagccattg gtcgttcagc cggggcacga
     1321 caacaacatc ttctgggatg gcttacgcca cgactcaaga gatactctat atcgcacacc
     1381 aggcggcgcg gtacccgaag gtacacctgt catcattcgc ttccgtacct tccacaatga
     1441 tgtaaccagt gttcaattgc gcgtattcag cgtcaacgca ggcgggcaat ccctggttaa
     1501 tatgacactg gcagctactg atgtatcctg ttatgaggaa ctggattaca cctgcgatta
     1561 ttggtcagcc acgctggcaa atgcgaacgc ggataacctg tggtaccggt tcatcgtcac
     1621 cgatggcagt gacaccgatt actacgacga caacaccgcc gcgctggatg gcggattggg
     1681 cgccaccacc gacgaaatcg aagatcatag ttgggcgctc atggtctatg aacccgactt
     1741 cacggctcca agctgggcga acgatgcgtt catctatcag atcttccccg accggttccg
     1801 caacggacgc agcaataacg atccgcacac gggtgacgtg cgctacgatg atccggtatt
     1861 ggcacttcca tggaacacct tgccagaagg ctactgccgc aattatgcgg atggcgacac
     1921 aaattgtccg tggcgctttg atacgacacc tccatctaac agcccgacca aggaacaacc
     1981 tcgcggtcgc gactatatgg gtggcgacct gagaggtgtg gatcaatacc taacctactt
     2041 gaagtcgttg ggtgtcaata cgatctattt caacccgatc tttgatgcgg gctcgaatca
     2101 ttcctacgat acgcaggatt acacgaagat cgatccgtat ttcggcacac aacaggattg
     2161 ggcaaatctg gtcaggcatg gcaaccagtt gggcatcaga atcgtgttgg atggtgtatt
     2221 caatcatatg tcctctgata gccccttctt tgaccgttat caccactatc cgacggtcgg
     2281 cgcatgtgag tcggttacat cagagtatcg ttcctggttc aatttcacag atgtccccgc
     2341 aggaacaggt acgtgtgtgg gtagtgatgg aacagccaac gcggcgaact atgagggctg
     2401 gttcggcttc gactccattc ccgtgctgaa taaaactgag gaggaagttc aggcctactt
     2461 ccttgataac cccaatagta tcgcgcgtta ctggctgcaa cagggcgccg gtggatggcg
     2521 catggacgtt tcaggtgacc ccagtttccc ggagggatac tgggaaaact tccgctctgt
     2581 cacaaagagc acgaaatctg acgctttgtt gatcagtgaa acctggcaga aagacagcac
     2641 cttgctccgt atgctgcgcg gcgacaggct cgataccacc atgaactatc gactgcgcga
     2701 tgcagtgatc ggtctgcttg ccccgggcaa cttcgactcg aaaggttttg cagatagtgg
     2761 tcgcatcatt gagccatcgg agtttgcagc ccgcatcgcc tcgatgcgtg aagattatcc
     2821 ggatgccgcc tattattctt tgatgaacct gctggatagc catgatacag aacgactgtt
     2881 atggacgttg acaccgggcg ctgagactcg cgcggagaaa gaattcaata ctccgaatct
     2941 cactgcaggc aaacaaagcc tgaaaatcgc ctccctgatc caattcacga tgcctggcgc
     3001 gccgaccgtg tactatggcg acgaagtagg cgtcaccggc gatgatgacc cggatgatcg
     3061 acggacgtat ccgtgggcgg atcgaggcgg ttcgccggat aacacgctat tcgctcatta
     3121 tcaaacattg agtagccttc gcactcagta tccatccttg accgatggcg atattcgaat
     3181 tctattggct gacgatgcct cagataccgt cgcttatgga cgcaagacga atgcccaggc
     3241 ggcggtcgtg gtgatcaatc gtggcagcca gtctcaagcc gtatccattc ccgttgccgg
     3301 ttatcttcca gatgggaccg tgttgaccag agcatacggc gtgggtgtga gcggaccatc
     3361 ctctcttacc gtcactgacg gtcagttgac cggaaccatt ggtgccaaga gtgcttggct
     3421 gttggtcaca ggcacggtcg atttacagcc gactgctgca ccgtctggtt taactgtcag
     3481 caacgaaggt gacgcggaag tgagcctggc atggaatagc gttgctggcg caacgggcta
     3541 caatgtgtat cgcagtcctg tcacaggcgg cggctatgtt aaagcgaacg atactccggt
     3601 gactgacaca acctttattg atacaggttt gcgcaatgcg caaaaatatt tctatgtggt
     3661 gaccgcgctc gactcagcag gtaatgaaag cgcgtactcg aatgaagtta atgcaactcc
     3721 gcatttggtg atcggttggg caaatcttca atggccgcca accatgactc atacgatcag
     3781 tgtgactgac cgcaccgatg atgcctacgg tcaggtctgg attgatggtg caaccaacca
     3841 accgggtgcc accgaaagcc tgcgtgccca acttggcttt ggttcggaag ggacaaaccc
     3901 cgctaccgac tccagttggg tctgggtaga ggccacgttc aacgtggatg cgggaaataa
     3961 tgatgagttc aaggccagcc tgttgcctga aacgaccggc acatttgatt atgtgtatcg
     4021 ctacacaacc acgaatggtc aggattggct ctatgccgac ctcaatggtc caatccccca
     4081 gggtgatgtt ccgcccaacc ccggcaagct gacggtgaac gcaagcggtg atacgactcc
     4141 acctgccggg ccgacgaatc tggatgtggt ttcagcatca cctgtcggaa ttgcgcttga
     4201 atgggatgca gttgcaggtg acccaacctt atacggttat gaagttctac gcagcgcctc
     4261 gagcggtggc ccatacgaca cgcttgcact cgtcacgggc acaagctaca ctgatacggc
     4321 cgtcgccgaa aacgaaacct atttctatgt cgtacgcgcg gttgatactt cgttcaatcg
     4381 atcagacaat tcaaatgaag ttgaagcgac cgctgaactg cggacagtga ctctggtctt
     4441 caatgtcacg gtaccggcaa cgactgacgc aactgggcgc tctgtatata tcgcaggttt
     4501 cctcgatcgg ttggatggaa ataacccaca atgggatccg ggcggcgtgg tgctgacacg
     4561 cgtcgatgcg acccactgga cgattacctt caccggcaaa gagtcaacac agcttgagta
     4621 taaatatgca cttggctcgt gggactatgt tgaaaaagat gtcgcctgtg gtgaaatcaa
     4681 caaccgcatg ctcacgttaa gttatggcac aaccggcacg cagacggtcg aagatacggt
     4741 acctaactgg cgtaacgtcg ccccctgcgg caattaaagt acgcgctcgg cgtattgagc
     4801 aaaatcaaaa tagagaccca cttgtgggtc tctattttct ttggttggca gagggtgatg
     4861 gataattact gaaatataga ttggcaaggc ttccgagaaa gaagacaaac aggatcgcca
     4921 caacgacctc cgcgatccag ccgatcaccg ggaagaacgt gaggatctcg agcgctacat
     4981 ctttcagtgc tgtcgaaatc ccccaaattg cagcggcaga ataaccgatc tctttgagtc
     5041 caagctgaaa attgttgacc ttcgacagat gcaccagcat gaacacctcc agcgcggtca
     5101 acaataggga cgtgccgggg attggcgcgg tgatggcgga tatcacacct gcgaccaacg
     5161 tatagataag gataagcctg gtgcctggtt tcatgcgcag tgattagacg gaagacttca
     5221 ttttcgcatc aacgctactt tatatgggta caaagtagct cgcatcactc caccggcgac
     5281 tgctgggtcg ttctccatga tgcttcgcgc cgctgattca tcttccgctt cgaaaatggc
     5341 gatcccgaag atattctcgt ccatggtttg cgtcctgccg actagaatca tcacaccttt
     5401 atctgtcaga tcactcacat aagcaaagtg acgcgagacc gtctcctgtt cctgctccgt
     5461 cgcgccttct gtgatcattt cgatacgcgt tggtttgaga tagtaaagcc actgaggttt
     5521 gttatcctca gtgcgcttgt gcttctcgac gatgatctcc ttattcagaa ttcggacgtc
     5581 tccgaccatc aaaagacgca tgtctttgac gagatagtca gccagttcct catctgaaaa
     5641 atcatcgcct tctatgtcca gtcgaaaatc ctgcccctga atcccaccgc cgttggtaaa
     5701 gtcaatctcg aaatcaaatt taacgcgttt ttccacctgg cactcctttg gatcagggaa
     5761 taagccgcga cagtaaaaaa gcaatgccgg tcaggatgag agctgcaagg atgccccaca
     5821 tcacaccgcg cattccggcc cgagtatccg gctcaaaggc ggaggacgtt tgctgaatag
     5881 cgggctcaat cccttcatcc ttcccatccg ttccggcttg tttctgttct ttttcataag
     5941 cgcgacgcac cttcggtggc atctcataca cgctgtaata ggtcttccct tgaaatacaa
     6001 ttttaggcat acgctcacct caattcccac ttataccaaa tatcattctc accggcatgg
     6061 ggactttccc tggcctgcga tgttcaatcg aaactcccgt ccagcgccaa agtccagcaa
     6121 taaatcttcg gcgtttcgct caaggtttta gccattcgat caacaaatgg gctatattct
     6181 tcatgacaat tttcaggttt gtatcaaata caacaggtgc ttcgtggttg gctgtgtcgg
     6241 tagcgccgcg aatttctaca taagggatgt tgctaaatgt gctggcacgt gctccaccta
     6301 cgccttccca ggcaaccgct aaggcattgg tcatctcacg tagttctgca ccccgtacaa
     6361 cgtcgatcac atcttcatcg cctcctgcca taatcccgaa gtgaatcctg aagtccgcat
     6421 ctcttaattg caaggctttg atttgctcaa tgctttttct gtcgcctgca aagctcggct
     6481 tgggacgctg ggaaaatttg ttgttgaaat catgttccag cgtgctggtc gcgatgatga
     6541 tatcgccaac tgctacctca ggagccagtg cacccgctgc gcccgcgcag atcaccagat
     6601 caaattgagc gtgatccaat aggtgttggg tttgaatgcc aaattgcgtt ttgccatgtc
     6661 cgccgcgagc caaagtgacg ttgagttcgg gaaaacgata aacatccagc cttccaagtt
     6721 tgccaatgtg ggatttcagt ccgcgagaag ctaaactact gtgaaggtca tcatgttctt
     6781 cctgcaatgg ggtaacgatg aggatgcttt gtgacatgga atgttctgca ggtaatttaa
     6841 atttatccta ttgcttgtcc ataaaactct gtaaacccac attgctcaca acaaaatgcg
     6901 accagaggtt tacgcgattt tgacagtggc ttgcccgaaa acattttgga gagatcaggc
     6961 ccggattcat catctgcagc gaagacgtac atatacttcc cgcctggagt atattcgttg
     7021 tatttcaaaa tccgcccctg agtcattgta ccgttgcatt tcggacaagt tttcgcgttt
     7081 gccatttttg tctccaatgg tagagaggat aaacgattgg cgtagcttat cgtccatcct
     7141 ctactatcag gtttgcatct tctcccattc atccagcttc tgatccatct ctttttggac
     7201 gcgttcatat tctgtgccta gtttcgcggc ttc
//
LOCUS       DASTJP010000646         7752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_54868, whole genome shotgun sequence.
ACCESSION   DASTJP010000646 DASTJP010000000
VERSION     DASTJP010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54868"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..725
                     /locus_tag="VFQ23_18925"
     CDS             216..725
                     /locus_tag="VFQ23_18925"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9908730.1"
                     /translation="MQLKEYLEQLYDYNYWANKRYLAVAETLTEEQLFLKQRHSWDSV
                     HAVLVHMMSSERMWPQRWRGETGTFLDAKDFPTMTSIRDYWVDVEKAMRSFLAEQSEG
                     SLLQDVSYTNPRGETFTLPLWNMMVQPPNHNTHHRGELAAMFALMSVPHPEEEVIQYF
                     LDKSGQKKF"
     gene            728..1351
                     /locus_tag="VFQ23_18930"
     CDS             728..1351
                     /locus_tag="VFQ23_18930"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HET9908731.1"
                     /translation="MNSKTSKWMEWAREIYSLSQVGLTYNKNDFDIERYKRLQEISAE
                     IIASQSELEMETILESFSMQAGYVTPKIDVRGAVVHNNKILLIQEKMDEKWAMPGGWA
                     DLGNAPASVAEREVWEESGYRVKAEKVVAVIDANRIQPMEFYHAYKIIFLCKLLDGEP
                     RVSHETLAVDFFDLNDLPPLSMYRTNENMLSEVFAHVENPDRRTLFD"
     gene            1351..2358
                     /locus_tag="VFQ23_18935"
     CDS             1351..2358
                     /locus_tag="VFQ23_18935"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET9908732.1"
                     /translation="MYSLLRSLLFRLDPETAHQFTLQLLRLGGVEPIYSILRSMYSMP
                     SRPIHAFGLTFKNPVGLAAGYDKNAVAVKGLSALGFGHIEVGTVTPKPQPGNPRPRVF
                     RLVEDEAVINRMGFPSHGADFVAKSLRLGNPLAIIGVNLGKNKDTPLDAAAEDYLSLM
                     RTFTPLASYLTINISSPNTIGLRRLQGREMLEQLLGAIQKEREGIALGRGGNAPVLVK
                     IAPDLTDEELDDAVGVILDKGMDGIIAANTTLAREGLRSNHQSELGGLSGSLLRVRSE
                     TMLRQLVNRIHGKIPIVSVGGIMNPKDAKRRLEMGATLIQLYTGLIYHGPGLVKKILE
                     H"
     gene            complement(2372..4474)
                     /locus_tag="VFQ23_18940"
     CDS             complement(2372..4474)
                     /locus_tag="VFQ23_18940"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9908733.1"
                     /translation="MQPDKIGRYEIKSELGRGGMATVYRGYDPRFEREVAVKVLPPEL
                     LHSDPQFKLRFDREAKIIAQLEHSSIVPVYDVDTDGDQPYFVMRYMSGGSLSERIKAG
                     VFSIEDATRILERVGPGLDEAHSKGIVHRDIKPSNILFDNRGTPYISDFGIAKLSQAQ
                     AGNVTGSAIIGTPAYMAPEQAQGDNIDGRVDIYALGIILYEMVTGRQPYEADTPMAVA
                     LKHITDPVPHILTANPKLPQGMDLVIQKAMAKNREERFANSREMVDALHAVMRGETTR
                     LQPVAPKTVKSEPAPPTVVAEKRSFNMLTVVIPVIALAVLAGGFFLFNGMNRPSATEA
                     PVSTFTSAPVTDTSEPTVAATEAVILPAETAIETATEAPPAATDTAAPPALPVLGGAD
                     KLAVVANNEIWLMNVDGSDLQVLTNDGGAKTDLQFLDSETLLFISGRTIKYLNINTGA
                     VDILTSFPTASSLNAFRVSHDGTQVMIALNNEIFVVPFDFEEIKNVNRKSHLLALDFC
                     IQPEGRTRSALVVREALWSHDDKLVAWLFKGVDAGNPSLQAEQVSILDITACTPSAID
                     QLDNFPALRFPVEGYQNRLMPDIDWDGNDLFVFNTDRRNDGWGELYVYDWVLHKAYHI
                     NPIEGACCYRDARWSPDGLYLLFAFQDIRLGAEAPNLLYYVPYGEIGTGTGFTPLPLE
                     DFFANPREAPQPALHPAP"
     gene            4748..5110
                     /locus_tag="VFQ23_18945"
     CDS             4748..5110
                     /locus_tag="VFQ23_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908734.1"
                     /translation="MDEKITIIEGPPPVFEAVNDGWALGLNEGSRLSVPALTRLRTFN
                     GPALVERCYRAWKDKSTIHLHYRNDMGVEQSAPILAARNVETVDGHVLLLWVYLDHDK
                     VEYELDKGDDEADEDNLE"
     gene            5333..6631
                     /locus_tag="VFQ23_18950"
     CDS             5333..6631
                     /locus_tag="VFQ23_18950"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET9908735.1"
                     /translation="MANDINAFAMAQKQFDRVAAMLNLDPGVIGALRWPMREFTFRIP
                     VRMDNGSLQFFQGFRVQHNDARGPNKGGIRFHRDETIDTVRALAMWMTWKCAVADIPL
                     GGGKGGIVVDPSTLSEREKEALCRGWVLQMWHNLGSRQDVPAPDVGTNSQMMGWMMDE
                     YSRIVGQYSPGVFTGKPIGGGGSQGRTEATGYGVMYTVREAMKHLNMDPTKSVAALQG
                     FGNVSQYAAIGFVEMLGGKVACVSCWDRHDKKSYTYSHKDGIDPRFLLSIVDGYGTVD
                     KKAAEEAGYIVEDGDAWISKEADVLIPAALEGQVNEDSIKKMSSRVKIVAEGANGPTT
                     PEADEFFKENNIFTIPDFLCNAGGVTTSYFEQVQNDANYYWERDAVLRRLDIKMTSAF
                     NAVLERSEKEKVYMRDAAYMVAIDRVVKAMELRGWLNAGW"
     gene            complement(6671..>7752)
                     /gene="gdhA"
                     /locus_tag="VFQ23_18955"
     CDS             complement(6671..>7752)
                     /gene="gdhA"
                     /locus_tag="VFQ23_18955"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET9908736.1"
                     /translation="FHPSVNLSIVKFLGFEQIFKNSLTTLPMGGGKGGSDFDPKGKSD
                     MEVMRFCQAFMSELFRHIGPDTDVPAGDIGVGGREIGYLFGYYKKIMNEHAGVLTGKG
                     TDYGGSLIRPEATGYGAVFLAAEMLGTRNQEIQGKTVAVSASGNVAQYTVQKINQLGG
                     KVITLSDSDGTIVDEAGVDAKKLDFVLDLKNVRRGRIQEYADHFKGVKYYAGKSVWDV
                     IRENGLKIDLAFPSAHENEIDGVHAEALIKNGCTLVCEGANMPSTADAMEVYRQHQVL
                     FGPAKAANAGGVATSGLEMSQNSLRLSWSREEVEERLHNIMKNIHRNCLEAAEAYGMP
                     GDYVAGANIAGFLKVANAMLAYGVV"
BASE COUNT         1898 a         1869 c         2071 g         1914 t
ORIGIN      
        1 aggtctccca aaagctgaat ccatcaggtc atgttgtggt ggaagcctcc aacctgaaaa
       61 agaacggtca agtcacaacg ttagcctggg atattacgcg cgagctttca caaatctttc
      121 acttccacgg cgagactgtc atttgctggg acaaatatgg ctacggctag aaccacagct
      181 attgtttggt tttttcgaaa gtttgaggag catgtatgca actcaaagag tatcttgagc
      241 agctttatga ctataactac tgggcgaaca agcgctatct ggcggtcgct gaaacgctga
      301 cggaagaaca actttttctc aaacaacggc acagttggga tagcgttcat gccgtgctgg
      361 ttcacatgat gagttcagag cggatgtggc cccagcgctg gcgaggagaa accggaacat
      421 ttctcgatgc taaagatttt ccaacgatga catcaattcg tgactattgg gtggatgtcg
      481 agaaggccat gcgcagcttc ctcgccgagc agtcggaggg atcactcctg caggacgttt
      541 cctataccaa tcccagaggc gagacgttta ccttgccgtt gtggaatatg atggtgcagc
      601 cgccgaatca caacacacat caccgcgggg aactggcagc aatgtttgct ttgatgagtg
      661 ttccgcaccc tgaggaggaa gtcattcaat attttttgga taaaagcgga cagaaaaagt
      721 tttgattatg aactcaaaga cttcaaagtg gatggaatgg gcgcgtgaaa tttactcact
      781 gagtcaggta ggtttgacgt ataacaaaaa tgattttgat atcgaacgat ataaacgctt
      841 acaggaaatc tcagcggaga tcattgcaag tcagtctgaa ctcgagatgg aaacgatatt
      901 ggaaagtttt tcaatgcagg cgggatatgt aaccccgaaa attgatgtgc gcggcgcggt
      961 ggtgcataac aacaagatct tattgatcca ggaaaaaatg gacgaaaaat gggccatgcc
     1021 aggtggctgg gctgacctgg gaaacgcgcc ggcatcggtc gccgaacgcg aagtctggga
     1081 ggaaagcggg tatcgtgtga aagccgaaaa agtggtggca gtgatcgatg cgaatcgcat
     1141 ccagccgatg gagttttacc atgcttataa gatcatcttt ctttgcaaac ttctggatgg
     1201 agagccgcgc gtcagccacg aaacattagc agtcgacttc tttgatttga acgacttgcc
     1261 acccttatcg atgtatcgca cgaatgaaaa catgctgagt gaagtgtttg cgcacgtcga
     1321 gaatccagac cgccggacgt tatttgatta atgtattcgc ttttgcgctc gcttctcttt
     1381 cgccttgacc ctgaaactgc acaccaattt actttacaac tcttacgcct tgggggagtt
     1441 gaacccattt attcaatctt acgtagtatg tactcgatgc cctcgagacc catccatgca
     1501 tttgggctga cgttcaagaa tccggtgggg ctggctgccg gctatgacaa gaacgccgtt
     1561 gcggtcaaag gactttctgc cctgggcttt ggtcatatcg aggtaggaac cgtcacgccc
     1621 aagcctcagc cggggaaccc acgtccgcgc gtgtttcgcc ttgtagagga tgaggcggta
     1681 atcaatcgga tgggatttcc aagccatggt gcagactttg tagcgaagtc actgcgactt
     1741 ggcaatcccc tggcaatcat tggagtcaac ttaggcaaga ataaagatac accgttggac
     1801 gccgcggcgg aagattattt gagcttgatg aggactttca cacccctggc gtcttacctg
     1861 acgatcaata tatcttcacc gaatacgatt ggcttgagaa gactgcaagg acgcgaaatg
     1921 ctcgagcaat tattgggcgc cattcaaaaa gagcgtgagg gaatcgcact gggtcgtggt
     1981 ggaaatgcac cggtgcttgt taagatcgca ccggacctga ctgacgaaga actggacgat
     2041 gcggttggag ttatcttaga caagggtatg gatggcatca ttgccgcgaa tacaaccctc
     2101 gcgcgcgagg gattgcggtc aaatcatcag agtgagttag gtgggttaag tgggagcctg
     2161 ctcagggtcc gtagtgagac gatgctgcgt cagctagtga atcgcataca tggaaaaatt
     2221 cccattgtga gtgttggcgg aatcatgaat ccaaaggatg cgaaacgaag gctggagatg
     2281 ggggctactc taatccagct ttacaccgga ttaatttatc atggccctgg tttggttaaa
     2341 aaaattctag agcactgatg ctccagaatt tttaaggcgc gggatgtaag gctggctgcg
     2401 gtgcctccct ggggtttgca aagaaatctt cgagaggaag cggggtaaaa cctgtgccgg
     2461 tgccaatttc cccataaggt acataataaa gaagattcgg agcttctgca ccaagacgga
     2521 tatcctggaa agcgaataac agatagagac cgtctgggct ccagcgtgca tcgcgataac
     2581 agcaggcgcc ttcaatgggg ttgatatggt acgctttgtg caacacccaa tcatacacgt
     2641 aaagttcacc ccacccatcg ttacgccgat cggtattgaa aacaaacagg tcgtttccgt
     2701 cccaatcaat gtctggcatc agccggttct ggtatccctc caccgggaaa cgcaacgcgg
     2761 ggaagttgtc gagttgatcg atcgctgagg gtgtacacgc agtaatatcc aggatgctta
     2821 cctgttcggc ttgaagcgat ggattgcccg cgtccacgcc cttaaagagc catgccacaa
     2881 gtttatcgtc gtgtgaccaa agcgcctccc gcacgaccag cgcggatcga gtccttcctt
     2941 cgggctggat gcagaaatca agcgccagaa gatggctttt ccggttgaca ttctttattt
     3001 cttcaaaatc aaacggcacc acaaatattt cgttgttgag agcgatcatt acttgtgtgc
     3061 catcatgaga gacgcggaag gcattgagtg aagaagctgt agggaagctc gtaagaatat
     3121 ccaccgcgcc tgtattgata ttgagatatt tgatggttcg tccactgata aagagaagcg
     3181 tctcactatc caaaaattgg agatcggtct tggcgccacc gtcatttgtc agcacctgca
     3241 gatcgcttcc atcgacattc atcagccaga tctcgttatt tgccacgacg gcgagcttat
     3301 cggcgcctcc aagtacgggc aaggctggag gtgcagctgt atctgtcgcg gctggtggtg
     3361 cctcggtagc tgtttctatg gctgtttctg ccggaaggat caccgcttcg gttgcggcta
     3421 cggtaggttc agatgtgtct gttaccgggg cagatgtgaa tgtgctgact ggcgcttcgg
     3481 tggcggatgg acggttcatt ccgttaaata aaaagaagcc tccagcgagc actgccagcg
     3541 cgattaccgg tattaccacg gtcaacatat tgaagcttcg tttctctgcg accaccgtgg
     3601 gcggggctgg ctcgctctta accgtctttg gagcaacagg ttgaagcctg gttgtttccc
     3661 cgcgcatgac tgcatgaagc gcgtcgacca tttccctgga attggcgaac cgttcctccc
     3721 tgttttttgc catcgccttc tgaatcacca gatccatgcc ttgtggcagt ttggggttgg
     3781 ccgtcagaat atgcggcacc gggtcagtaa tatgtttaag agcaactgcc atgggcgtat
     3841 cggcttcata gggctgcctg cccgtgacca tttcatacag aatgatgcca agcgcataaa
     3901 tatcaacgcg tccgtcaatg ttgtcgccct gggcctgttc aggagccatg taggccggcg
     3961 tgccgatgat cgcactgcct gtcacatttc ccgcttgtgc ctgagataac ttcgcaatcc
     4021 caaagtctga gatgtaagga gtgccgcggt tgtcgaacag gatgttgctg ggcttgatat
     4081 cccgatggac gatgcctttg gagtgagctt cgtccaggcc gggtccgact cgttccagga
     4141 tgcgagtggc atcttcaatt gagaacacgc ccgctttgat ccgctcggaa agggacccgc
     4201 cgctcatgta gcgcatcacg aagtaaggct ggtcgccgtc cgtatctacg tcatagacgg
     4261 gaacaatcga ggaatgctcc aattgtgcga taatctttgc ttcacgatca aaacggagct
     4321 taaattgtgg gtcagaatgg agaagttcgg gcggaagcac cttgacggca acctcgcgct
     4381 caaaacgcgg gtcgtagcca cgatagacgg ttgccattcc cccacggcct aattccgatt
     4441 tgatttcgta gcggccgatc ttgtccggtt gcataatggc tcaagtatac ctaccccaaa
     4501 gtaccagcgc aagcgctaat cgtagcaact taatgatgaa atcgttaata tcaacgctgt
     4561 aggattttga aacaaaatgg cgggtcaggt cgtcttgaaa atatgccaaa gaatttctgg
     4621 caaagtcaaa aggtttatcg tcagggcgga tgcgatatta ggaggcacat gaaactagat
     4681 gataggaaga tgaaacaatg caatcgtaag attgttgctt ttccttttgt ggtatattgg
     4741 cggcactatg gatgaaaaaa ttacaatcat tgagggccct ccgcctgtgt ttgaagcagt
     4801 aaatgatggc tgggcattgg gtttaaatga aggttcccga ttatcggttc cggcgttgac
     4861 gcgtttacgg acgttcaacg gtccggcact ggtggaacgc tgttaccggg catggaagga
     4921 taaatcgact atacatttac attatcgcaa tgatatgggt gttgaacagt ctgcgcccat
     4981 tctggcggca cgcaatgtgg aaacggtgga tgggcatgtg ctcctcttgt gggtctatct
     5041 ggatcacgat aaggtcgaat atgagctgga taaaggggat gacgaagcag atgaggacaa
     5101 tctcgaataa agaaaatcga ccaggcgcag gcctggtctt ttcttctaac gaacagtaac
     5161 cttaactaca ttgacgagta tttgattctc gtgataacgt taagaaaaca atatatgtat
     5221 tgggcaatta cacgatgaat gtatggtcca cataacctga agttaaaagc gggtttcctt
     5281 tccgggatac ccgcttatct ttttaagttt aagtttctca aggagactga acatggcgaa
     5341 tgatatcaat gcctttgcga tggcgcaaaa acagttcgac cgcgtcgccg caatgctcaa
     5401 ccttgatccc ggcgtgatcg gcgcgttgcg ctggccgatg cgcgagttta cttttcgtat
     5461 ccctgtgcgc atggataacg gctccctgca attctttcag ggattccgcg tccagcacaa
     5521 tgacgcgcgc ggccccaata agggaggtat ccgctttcac cgtgatgaga cgatcgatac
     5581 cgtacgcgcg ctcgcgatgt ggatgacctg gaaatgtgcc gtggcagata ttcccttggg
     5641 cggcgggaaa gggggcatcg tggtcgatcc atccacgctc tcagagcgag agaaagaagc
     5701 gttgtgccgc ggctgggttc tgcagatgtg gcacaattta ggctcccggc aggatgtgcc
     5761 tgcacccgat gttggaacca attcacagat gatgggctgg atgatggatg aatattcacg
     5821 catcgttggg cagtattccc ccggcgtctt taccgggaag cccatcggcg gcggtggttc
     5881 acagggacgt accgaggcca caggctatgg tgtgatgtat acggtgcgcg aggcaatgaa
     5941 acatctcaat atggacccga ccaagtccgt tgctgccttg cagggatttg gcaacgtctc
     6001 acaatatgcc gcgattggtt tcgtggaaat gctgggtggc aaggtggcct gtgtttcctg
     6061 ttgggaccgt catgataaaa aatcctatac ctatagccac aaggatggaa tcgatccgcg
     6121 tttcctctta tcgattgtgg atggatatgg cacggttgat aagaaggccg ctgaagaggc
     6181 cggatacatt gtcgaagatg gagatgcctg gatttcgaaa gaggcagatg tgctcatccc
     6241 ggctgccctt gaaggtcagg tgaatgaaga ttcgatcaag aagatgtcca gccgggtgaa
     6301 gatcgttgcc gagggtgcta acggacctac aactccggaa gcggatgaat tctttaaaga
     6361 gaataacatc ttcaccattc cagatttcct ctgtaatgcc ggtggtgtga caacctctta
     6421 tttcgaacaa gtgcaaaatg acgccaatta ctactgggag cgcgatgcgg tattgcgccg
     6481 tctggatatc aaaatgacct ccgcgttcaa cgccgtactc gaacggagtg aaaaggaaaa
     6541 ggtctacatg cgcgacgccg cctatatggt agcgattgat cgcgtcgtca aagccatgga
     6601 actgcgcggc tggctcaacg caggctggta acaaagtgac ggtcaccctc agggtgaccg
     6661 tcacttcttt ctagacaacc ccataagcaa gcatcgcatt cgcaaccttt aggaatccgg
     6721 cgatattcgc gccggctacg taatcgcccg gcattccata ggcttcggct gcttcaagac
     6781 aattcctgtg gatgtttttc ataatattgt gtagtctctc ttccacctct tcgcgcgacc
     6841 aggacaagcg caggctgttc tgactcattt ccagaccaga ggtcgctact cctcctgcgt
     6901 tcgcagcttt ggcaggtcca aacaagacct ggtgttgacg ataaacttcc atggcgtctg
     6961 ctgtggatgg catgttggcg ccttcacaaa caagagtaca tccgtttttg atcagcgctt
     7021 cggcgtgaac accgtcgatc tcattctcgt gtgcggaggg aaaagccaga tcgatcttca
     7081 gtccgttttc cctgatcacg tcccaaacac ttttgcccgc atagtactta acacctttga
     7141 agtgatccgc atattcctgg atgcgtccgc ggcgtacatt cttcaaatcc agcacaaagt
     7201 ccagtttttt tgcatccaca ccggcttcgt caacgatcgt gccatctgag tctgagaggg
     7261 tgatcacttt ccctcccaac tgatttattt tttgcaccgt atattgcgca acgttaccgg
     7321 aggcactgac tgccacagtc ttgccctgga tttcctgatt ccttgtcccc aacatttcgg
     7381 cagccaggaa aaccgcgccg tatccggtgg cttccgggcg gatcaagctt ccaccgtagt
     7441 ctgtgccctt gcccgtcaac acgccggcgt gttcattcat gatcttcttg tagtatccaa
     7501 acagataacc gatttccctg ccgcctacgc cgatatcgcc cgccggcaca tcggtatctg
     7561 gtccgatgtg tcgaaagagt tcgctcatga acgcctgaca gaagcgcatc acttccatgt
     7621 ctgatttgcc ttttgggtca aaatctgaac ctcctttgcc gccgcccatc ggaagtgtgg
     7681 tcagtgagtt cttgaagatc tgctcaaaac caagaaattt gacgatgctt aggttaaccg
     7741 acggatgaaa ac
//
LOCUS       DASTJP010000647         4593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_581990, whole genome shotgun sequence.
ACCESSION   DASTJP010000647 DASTJP010000000
VERSION     DASTJP010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581990"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..700)
                     /locus_tag="VFQ23_18960"
     CDS             complement(167..700)
                     /locus_tag="VFQ23_18960"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET9908737.1"
                     /translation="MDAKLLNPFVQAAVEVLEVEVGATISRSNISLQKSALTSDDITV
                     LINLIGDVYGVVMYGISMPTGLRMVSQIMGQEFAELNSLAQSGIAELGNVMSGKATVR
                     FSEAGYHSNISTPTVLIGSGIQISTLDFPRIVVPLQTQFGLFTAHLALREKTPGTPHK
                     PDDFISLVEVPASQKSS"
     gene            complement(884..1678)
                     /locus_tag="VFQ23_18965"
     CDS             complement(884..1678)
                     /locus_tag="VFQ23_18965"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET9908738.1"
                     /translation="MDADVYTQVKASIRRRLNIDLTNYKDEQMKRRLDAWLVRVHAST
                     WNDYFGLLSSDPTELERFRNYLTINVTEFFRDQNRWDFLRQEILPYLLTQSTGNRPVG
                     ELKLWSAGCSIGAEPYTLAIMMNEANPLQKYSLLATDLDRGALHKARSRGPYLESDIR
                     NLDTALLQKYFTPAAPFYIKESLQKSIRFKEQDLLADPFERSFDLIVCRNVVIYFTAE
                     AKEMLYAKFSDALRPGGVLFLGGTELISGPTKFGLQNFGISFYKKV"
     gene            complement(1710..2798)
                     /locus_tag="VFQ23_18970"
     CDS             complement(1710..2798)
                     /locus_tag="VFQ23_18970"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET9908739.1"
                     /translation="MTDPMLPQAGMKSPDAVRVMVVDDSAFMRFAIAQHLNESPQIEV
                     VGSAYNGKEALRLIPVLQPEVITLDVEMPQMDGLTTLRQIMADFPRPVIMFSSLTKEG
                     AAETIEALMLGAVDFIAKPDNRLNIRKVMNDMVSKILCAARARIKPVPTNRPRSFPGA
                     QANHLDKPLRAHQKSMPVILIGSSTGGPGALNEIIPALPATLPSPVLIVQHMPAGFTR
                     SLSDRLNSISPLRIKEAEPGDRLMAGQVLVAPGGFHMVLDKEERILLHQKAAVHGVRP
                     AVDVTLTSLVQRFGKNVIAVILTGMGSDGTHGASLLYGEGGYVIAEHESTCVVWGMPR
                     SVVEAGAASAILPRPQIASALEAVLQKR"
     gene            complement(2806..3162)
                     /locus_tag="VFQ23_18975"
     CDS             complement(2806..3162)
                     /locus_tag="VFQ23_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014476812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9908740.1"
                     /translation="MAKVLIVDDAEFLRVRLTKMLDTDGYEVFQAENGLKAVSTYKEI
                     HPDVVLMDVTMPEMDGLTALKEIVGFDPRARVVMLTALGQESVVLEAVQSGARDFVVK
                     PFEQDRVMKAIRKLLA"
     gene            complement(3184..3672)
                     /locus_tag="VFQ23_18980"
     CDS             complement(3184..3672)
                     /locus_tag="VFQ23_18980"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HET9908741.1"
                     /translation="MKEINVGLGEQAISRNPDDVLVAYGLGSCVGVVMVDPRSHISGL
                     LHAVLPRPVDGGRGEHNSSKYVEGGIEGLIASMVREGAYKNRLVVRIVGGANMLISSG
                     LTRSFDIGTRNIEAAQMTLDRLRMPIVSAEVGGHTGRTVRVYVADNRVTVRVVGQKEY
                     EV"
     gene            complement(3675..4289)
                     /locus_tag="VFQ23_18985"
     CDS             complement(3675..4289)
                     /locus_tag="VFQ23_18985"
                     /inference="COORDINATES: protein motif:HMM:NF016398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HET9908742.1"
                     /translation="MDEVISFAPHVMERLNSIAKEGIDNAAYGFSEMVGRKIQFSKPK
                     ATLVPILTIPQIVGKPDDDAVGIYLRFMGDLAGQIMMIVPHHKALELVDLLMDVSQGT
                     TQHLGSLERSALGELGNLCGSFFLNSIAKMVGADFRPSPPAVMVDMVGAILDVVVVTT
                     GGIGEQALLLQANFMDGSRCVEADFWVIPDMNSLHDVVMKNSGS"
     gene            complement(4303..>4593)
                     /locus_tag="VFQ23_18990"
     CDS             complement(4303..>4593)
                     /locus_tag="VFQ23_18990"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9908743.1"
                     /translation="VILLRGSVLPLVHLSDVFGFKNGREERKHFFAVVIQFGKERFGL
                     IVDSLIGEEEVVVKPLGTFIGDIPGISSATILGNGQVALIVEVFNLFKLVGV"
BASE COUNT         1181 a         1253 c         1029 g         1130 t
ORIGIN      
        1 cacagtcaca tcgtcagcca aatatggacc attttcgaga cgcaaatctc cgcgtgcaac
       61 tgtttcatgc atctcgagac tcaggatctc acttgtagcg ctgacaaaag gatttagaaa
      121 cttaacattc atcgcgggct ccaattcatg ttattcgatc gagtcgttag gaactctttt
      181 gggaggcagg cacctccacc aacgaaataa agtcatctgg tttgtgtggt gttcccggcg
      241 tcttttcccg taaggcaaga tgcgctgtga aaagaccaaa ttgagtctgc aatggcacca
      301 cgatccgggg aaaatcgagg gtggagattt gtatgccact tccgatcaga acggtgggag
      361 tggaaatatt ggaatggtag ccggcttccg agaacctaac ggtcgccttc ccactcatca
      421 cattgcccag ttccgcgatc ccactctgtg caagggagtt aagctcggcg aactcctgac
      481 ccatgatttg ggacaccatc cgcaggcccg taggcatgga tattccatac atcaccaccc
      541 catatacatc cccaatcaga ttgattagga cagtaatgtc atctgatgtc agggcagact
      601 tttgtaaaga gatgttgctg cgggaaattg tcgcgcctac ttctacttct agcacctcaa
      661 ctgcggcctg gacaaaagga tttagtaatt tggcatccat ataatttcta cctcgtaggg
      721 cagcatgaag actctgccgg tttcattcga aatgaatttt ttcgcctctg ttaattgccg
      781 gggaacctgt cctgtcagga gcaatacgtc ttcccatgtc agctccaggc atttaaagcc
      841 tttggattca tgggataatg aagttcatcg gacccaattc caactagact ttcttataaa
      901 aagaaatccc aaaattctgg agtccgaatt ttgttgggcc cgagatcagc tcagtgcctc
      961 ccagaaataa cactccaccc gggcgcagag catcggaaaa cttcgcgtaa agcatttctt
     1021 tcgcttcggc tgtaaagtag ataacaacat tccggcagac aatgagatca aagcttcgtt
     1081 cgaaagggtc agccagcaag tcttgttcct tgaaacgaat acttttctgt aaactctcct
     1141 taatatagaa aggcgcagcc ggcgtgaaat atttttgaag caacgctgta tccaaattgc
     1201 gaatatcgct ttccaaataa ggaccgcgcg acctggcttt atgcaatgct ccccggtcca
     1261 ggtctgttgc cagcaaagaa tatttctgca agggatttgc ttcgttcatc ataattgcaa
     1321 gggtgtaggg ttctgctcca atcgaacaac ccgcgctcca gagcttcagt tcgcccaccg
     1381 gtcgatttcc agttgactgg gtcagcaaat aaggcaggat ttcctggcgg aggaaatccc
     1441 aacgattttg atctctaaag aattccgtaa cattaatcgt aagataattc cgaaaacgtt
     1501 cgagctccgt gggatcacta gaaagcagac caaaataatc attccacgta ctggcatgaa
     1561 cacgaaccaa ccacgcgtcg agacggcgtt tcatctgctc atctttgtaa ttcgtgagat
     1621 caatattgag ccttctcctg attgaagctt ttacctgggt ataaacgtca gcatccattt
     1681 catagtcctg tttgatagtg atggagggct cacctttttt gaagtacggc ttccagtgca
     1741 ctggcaattt gagggcgagg cagtattgca ctggctgcgc cagcctcgac cacgctgcga
     1801 ggcattcccc agacaacaca agtactttcg tgctcggcaa tgacatatcc tccctcccca
     1861 tatagaaggg aggcaccatg cgttccatcg ctgcccatgc ccgtcagaat gacggcaatg
     1921 acatttttcc cgaagcgctg gaccagcgag gtcaatgtga catccacagc cggcctcaca
     1981 ccatgtaccg cggctttttg atgcaagaga atccgttcct ccttgtccaa aaccatatgg
     2041 aacccaccag gtgcgaccag tacctggcca gccatcaggc gatctccggg ctctgcttcc
     2101 ttgattctga ggggggaaat cgaattgagg cgatccgaca acgagcgcgt aaaccctgca
     2161 ggcatatgtt gcacaatgag aacgggtgaa ggcaaggtgg ctggcaaagc cggaatgatc
     2221 tcgttcaacg cgccgggtcc gccagtggaa gaaccgatta agatcaccgg cattgatttt
     2281 tgatgagccc gcaacggctt atccaaatgg ttggcctgcg cgccagggaa agaccttggg
     2341 cgatttgtcg ggaccggctt tatcctcgcc cgcgccgcgc acaggatttt cgacaccatg
     2401 tcattcatta ccttgcgaat atttagacgg ttatccggtt tcgcaatgaa atccacggct
     2461 ccgagcatca acgcttcgat cgtttcggcc gcaccttcct tcgttagact actgaacata
     2521 atgaccggtc gtggaaaatc cgccatgatt tggcgcaagg tcgtcaaccc atccatttga
     2581 ggcatttcca catccaaggt gatgacttct ggctgcaaga cgggaattag tcgcagagct
     2641 tctttgccgt tatatgccga acctacaacc tcgatttgag gagattcatt caagtgctgg
     2701 gcaatggcaa aacgcatgaa cgcagaatcg tccaccacca ttactcttac ggcatccggc
     2761 gacttcatac cggcctgtgg cagcatagga tctgtcatga tttgcttatg ccagcagttt
     2821 tcgaatcgcc ttcatcacgc gatcttgttc gaatggtttg acgacgaagt cgcgcgcgcc
     2881 cgattggaca gcctccagca ccacagattc ctgtccaagc gctgtcaaca tgacaacccg
     2941 cgccctggga tcgaacccga cgatttcctt tagggctgtc agtccgtcca tttcaggcat
     3001 cgtcacatcc atcaagacaa catccgggtg gatttccttg tacgtggaca cagccttaag
     3061 tccattctcc gcctgaaaga cttcataacc gtcggtatca agcattttgg tcagacgcac
     3121 tcgcaggaat tctgcgtcat ccacgattaa tactttcgcc atacaattgc tccttttgaa
     3181 ttttcacact tcatattctt tttggccaac gaccctaaca gtcacacgat tatcagcaac
     3241 atagacacga accgtacgcc ctgtatgtcc gccgacctct gcggaaacga ttggcatcct
     3301 gagacggtct aatgtcatct gggctgcttc gatattccgt gtcccaatat caaaggatcg
     3361 cgtcaacccg gatgaaatca gcatatttgc tccaccaacg atccgaacga ccaatcggtt
     3421 tttatatgcg ccttccctga ccattgatgc aattaagccc tcgattcctc cttcgacata
     3481 cttggaggag ttatgttcgc cgcgccctcc atctactggc ctgggaagga cagcgtgtag
     3541 aagtccggaa atatgggaac gcgggtcaac catcacaaca cccacacagg agcctaatcc
     3601 ataagcaaca aggacgtcat ccgggttgcg actgatcgcc tgctcaccta aaccaacgtt
     3661 tatttctttc attttcagct gccggaattc ttcatcacca catcatgaag tgaattcatg
     3721 tcagggatga cccagaaatc cgcttccacg caacgagaac cgtccataaa gttggcttgc
     3781 agcaacaatg cctgttcgcc aatgccgcct gttgtgacaa caacaacatc caggatcgcc
     3841 cctaccatat ccaccatgac tgcgggaggg gatggcctaa agtcagctcc caccattttg
     3901 gcaatggaat tcagaaagaa ggagccacac aggttcccca attcaccgag cgcggatctt
     3961 tccagggagc ccaggtgctg agtcgtaccc tgtgacacat ccatcaagag atcgaccagt
     4021 tccagcgcct tatggtgagg aacgatcatc atgatttgtc cggccaggtc acccatgaaa
     4081 cgtaaataaa tacccactgc gtcgtcatca ggtttgccaa caatttgtgg tattgtcaaa
     4141 atgggcacca gcgtcgcttt cggtttgcta aattggatct tgcgaccgac catctctgaa
     4201 aaaccatagg ccgcgttatc gatgccctct ttggcaatcg agttcaacct ctccatcaca
     4261 tgcggcgcaa atgaaatcac ttcgtccata gttctccttt tgttacactc caactaattt
     4321 gaataagttg aacacctcaa caatcagtgc cacctggccg ttgccgagaa tggtcgcact
     4381 ggaaattcca gggatatccc ctataaacgt acccagtggc ttgacaacaa cttcttcctc
     4441 gccaatcagt gaatcgacaa tcaacccaaa ccgttccttg ccaaattgaa ttaccactgc
     4501 aaagaaatgt ttacgctctt ccctaccatt cttaaatcca aagacatccg agagatgaac
     4561 gagtggaagt acgctgcccc gcaacaggat gac
//
LOCUS       DASTJP010000648         9338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1124843, whole genome shotgun sequence.
ACCESSION   DASTJP010000648 DASTJP010000000
VERSION     DASTJP010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124843"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFQ23_18995"
     CDS             complement(<1..702)
                     /locus_tag="VFQ23_18995"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9908744.1"
                     /translation="MKRSFVLVVLLFLIGCSNIPASTPLAQIPEDCTTPGEIIRGELS
                     RESVGYTYSYRVYLPPCFSGESNLRYPVLYLIPGRGSGPEAWFASGLGKVIDQLILDG
                     EIPPFIIVTTENTDSDPMAEAIYNELIPAIESQYPIIDNRRYHAVAGGSLGGVAAYRL
                     AFQHPDAFSSAGIFGAGAISGEEERITSWLSAMNEENRVRVFMDCGDKDIFMLDRAEE
                     MKHLLDQAGVENQLHV"
     gene            complement(707..1882)
                     /gene="galK"
                     /locus_tag="VFQ23_19000"
     CDS             complement(707..1882)
                     /gene="galK"
                     /locus_tag="VFQ23_19000"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET9908745.1"
                     /translation="MELIEGITALFSKTFGEKPAHLARAPGRVNLLGEHVDYNDGFVL
                     PAAIDRATYIAFSPSDSVQTTLLAADLNQQVSFSPETISTKTQIDSSPLPEWALYPAG
                     VMWTLLAESVSTPAMNAVYASNVPRGSGLSSSASVEMAFMIGWQTLGQWKLPPMQQAQ
                     LSQKAENQYVGVNCGIMDQFASACGVENHLLLLDCRSLEWKPIPLPADVAVVIADTMV
                     RRKLTSGEYNNRRAACEEAVRLLQKDLPDIKSLRDVSVHDFNRLAGKLPEEVSKRARH
                     VVEEIERTNQAEALLKVRNIPRFGELMNECHKSLRDFYEVSCPELDVMVRIAQSLDGC
                     YGARLTGAGFGGCTVNLVAQENAEAFAQSLKEGYQIETGYQPDIYITGASSGAERLI"
     gene            1977..3158
                     /locus_tag="VFQ23_19005"
     CDS             1977..3158
                     /locus_tag="VFQ23_19005"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9908746.1"
                     /translation="MPGLNVQLDALRQSPTIAMGDRVISLKASGRDIIGLQVGDPDFA
                     TPQPVIDVAMQALQEGLTHYGPSRGYLDLRQAAASKLHRDNDLSYDPQREILVTHGGI
                     HAYYLAMQSILNPGEDVLIPDPSWGTHSNMAVMLRCNVIRVPAPAENGFIPYFETWEK
                     VLTPKTRVIVLNYPSNPTGAYPTRQYLQQLQDFAEKHNLWIISDAVYESLYYEEKPTS
                     AASFEGAKDRTILVNSLSKTYAMTGWRVGFLAAPQQVINNAVKAGQNSITCVAPFIQK
                     AAAFALTDPGVQQNVEDMRLAYAGRRDLVMRLSHELESEKVLVKPPQGAFYFFLDLRP
                     LKMTSLEICERILEEVGVGLVPGAAFGEQGEGFVRMTIAASDEDVETGFRKIVEWAER
                     Q"
     gene            3174..3983
                     /gene="pheA"
                     /locus_tag="VFQ23_19010"
     CDS             3174..3983
                     /gene="pheA"
                     /locus_tag="VFQ23_19010"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET9908747.1"
                     /translation="MRVAFQGEPGAYSEQAVFNYFSSVETQPCESFDTVFDAVVSNAC
                     DAGLIPIENSLAGSIHQNYDLLLHHDLHITGEYLLRVQHCLIAQPAVKKHEITKVISH
                     PQALGQCAAYLRNLGVKVEAVYDTAGSVKMLKESGMRDMAAIASRRAAEIYGMEILEE
                     GIEDNPENYTRFLAISKNAVKPEGESKTSIVFTLKNQPGALFKALSVFALRDIDLTKI
                     ESRPLKGQPWEYLFYIDFIGAAHEETASKALDHLNEYALMLRVLGSYARYK"
     gene            4041..5609
                     /locus_tag="VFQ23_19015"
     CDS             4041..5609
                     /locus_tag="VFQ23_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908748.1"
                     /translation="MLNTIRPYITGDEPFGPIDSVDIDSGDIQALKLLFEQHNWIYKN
                     LRNRPSIIIGRRGSGKTYYLRSVFFDKQYDFYVEIRTARVLGYIARVIQKMTKEVVFP
                     EMVSELWETILWIAVFSELRKHSVIASEDLNLINAYLDKMSVLDNDNVEDVLWNVAAM
                     LDEIMNKNPKTMASDVLRGYNRITIDHAKAAVVRNLESSKKNFVILMDSLDDFHLDID
                     SVARSLQGLLKVVGAMNKPRDVVDIRFCLPSEVYHRFIKISSNPNKDFRRALKLQWTA
                     AELILIAAQRLMLYLMLYHPDFVKTILPLEPTRRADALKLFQAVFPEKITNQSGFQEE
                     TISYILRHTQLLPRHFLMLLNSIFKGSNTAQRKNPFPISQNRIVSGIRQMEEFMVGEI
                     FVAFKLNYPTAEETCRRCLPELGHKFTQADLHKVFTRHGKAVFGSDNLFEFQRMLLEI
                     GALGRVIPGKENDVYIKGYFEYTVAHELVLSQDDELCIHPLFSGIFHRGGKDRPVYPY
                     GSVLDDEDYRDSFE"
     gene            complement(5680..6378)
                     /locus_tag="VFQ23_19020"
     CDS             complement(5680..6378)
                     /locus_tag="VFQ23_19020"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9908749.1"
                     /translation="MKHSIKQTFSIFATLVIVVSLASCNLIQSGADEYNDLVALVQGV
                     QAAASNFQLVTDTQYAKIQAKTQITKDYYDAVSNSGEVWTGNFRSEQELLNELLRNYR
                     DENGQPLDPTQLNLADLQNKGALPDSLGEGFTLFVNAVVQAPPPVPAPEVTLALGDTI
                     DEGMNTIQLAGTDWNDAVQAYNTRRAQIPGEVVARVSEYFGFELPVAFPYYQGANPGA
                     PVQNPLGTPPTSTP"
     gene            complement(6397..7302)
                     /locus_tag="VFQ23_19025"
     CDS             complement(6397..7302)
                     /locus_tag="VFQ23_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908750.1"
                     /translation="MGANLAYQAYKLVALVALALSALGYTTPVRAQTTDELCGNGSPP
                     DYGLSYSLLQPHWTVGWEGYATEQAVDRVDRVFDRLDEDAIAQTMILILPQDQVGIRT
                     NCAVHFLRYMELGLPTGARKDNGFVFLIVVEPDDIDVHYAVGLGLPALTAPELTNINR
                     AVEDSYQSTHSMDQALLTLASEFSTAARSNYDPAPSAVVTPEVTDQPVGPAALVALCG
                     QFCLGLLLFVFLAWMFSQLGGGGRRYYTPQSNPWQSNPWGGSSWGGSPWRSFPSRGFP
                     SSGSRGFPSPRMRGGSGSGRSGRTN"
     gene            7552..8064
                     /locus_tag="VFQ23_19030"
     CDS             7552..8064
                     /locus_tag="VFQ23_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908751.1"
                     /translation="MAFLYEEFEIHTDLDTSNVYNRVLSVVETKQFWASPLSTAKPYF
                     GEVQSTSFDIRRVKWNSRAIRPVVVGKISSNNKGSYLRVRVRLNWFEFVFYMLVFGLF
                     GHFAFWYFIENLSADTLGWLGRTLSFFIPLTSFSIVYLLIFISVKVEAISAKQFFLEL
                     LNKNSNVEFP"
     gene            8593..>9338
                     /locus_tag="VFQ23_19035"
     CDS             8593..>9338
                     /locus_tag="VFQ23_19035"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET9908752.1"
                     /translation="MLLEATTVKTILQSVIREINADLETPVSLYLKLRGEGASFLLES
                     VEGGERIARYSFIGVQPRAEYILRDGQVEVKENGGTRTLQLNDDPTRFLQEEMSRFST
                     ERLPNAPRFTGGLVGYLGYESVRYFETTLKSQLTRAERSEPSGERSRSVPDGIYLLAD
                     TVVAFDHARRSIFLIANVLDGDLEAANQKLDQIAERIAQPLPAAGKTTVTPSEIRSNM
                     TQEAYETIVSEAKEHIAAGDIFQVVLSQRFT"
BASE COUNT         2411 a         2286 c         2313 g         2328 t
ORIGIN      
        1 aacatgaagt tgattttcca cgccggcttg atcaagcaga tgtttcattt cctcggcgcg
       61 atccagcatg aaaatgtctt tgtcgccaca atccataaag acacgaacgc gattttcctc
      121 gttcatggcc gagagccatg aagtgatacg ttcctcttca cctgagatcg cccctgcacc
      181 aaatatgccc gcgcttgaaa atgcatctgg atgttgaaac gccagacgat acgctgcaac
      241 cccacccagt gaacccccag ctacggcgtg atatcgtcta ttgtctatga tcggatactg
      301 actttcgatg gccggaatta actcgttgta gattgcttcg gccattgggt cgctgtccgt
      361 gttttcggtc gtgacaataa tgaacggagg gatctcgcca tctagaataa gctggtctat
      421 gactttaccc aacccagaag caaaccaggc ctccggccca ctgccacgcc cgggaataag
      481 ataaagtacg gggtaccgga gattggattc gccggaaaaa caaggcggaa gatatactcg
      541 ataactatat gtataaccga ctgattctcg agaaagctcc ccacgaataa tttcaccggg
      601 tgttgtgcaa tcctcaggga tttgggcaag aggtgtggag gcgggtatat tgctgcaacc
      661 gataagaaaa agcaaaacta cgagtacaaa gctgcgtttc atgagactat atcagtcgtt
      721 cggcaccgct cgatgcgccg gtaatataaa tatccggctg atatcctgtt tcaatttgat
      781 aaccttcttt cagggattga gcaaacgcct ccgcattttc ctgcgcaacc agattcaccg
      841 tacatccgcc gaagcccgcg cccgtcagac gcgcgccata acatccatcc agtgactgcg
      901 cgatgcgaac cattacatcc aattcagggc aggagacttc atagaaatca cgcaggctct
      961 tgtggcactc gttcatcaac tcgccaaagc gtgggatatt tctcaccttc aacaaagcct
     1021 cggcttgatt ggtcctctcg atttcctcta caacgtgacg tgcccgcttc gaaacttcct
     1081 ctggcagttt tcctgccaaa cgattgaaat catgaacact gacatcgcgc aacgatttta
     1141 tatctggtaa atctttttgc aagagtcgta ctgcttcttc acaggcggca cgtcgattgt
     1201 tgtactcacc ggatgtcagt ttacgacgca ccatcgtatc cgcaatcaca accgcgacat
     1261 cagctggtaa aggaatcggt ttccattcaa gcgaacgaca atcgagtaac agtaaatggt
     1321 tttccacgcc gcaggccgag gcgaattgat ccataatgcc gcagttcaca cctacatatt
     1381 gattttccgc tttttggctc aattgtgcct gttgcatggg gggcaacttc cattggccga
     1441 gagtctgcca gccaatcata aacgccatct cgaccgaagc ggatgaactc aagcccgatc
     1501 cgcgcggcac atttgatgcg tagactgcat tcatcgccgg agttgatacg ctctctgcca
     1561 acagcgtcca catgacgccc gcaggataaa gggcccactc cggtagaggc gaagaatcaa
     1621 tctgagtttt ggtagaaatg gtttcagggg aaaacgaaac ttgttggttc aagtctgcag
     1681 ccagaagcgt ggtctgaacc gaatcgctgg gcgagaaagc aatatacgtg gcacgatcga
     1741 tggctgccgg caggacgaat ccgtcgttgt aatccacatg ctcacccaga aggtttacgc
     1801 gtccgggcgc gcgtgcaaga tgcgcaggct tttctccaaa cgtttttgag aaaagcgctg
     1861 taataccttc gataagttcc atagtgattt attgtaacca gttatccgcc aggtttgcaa
     1921 acgatgaatt atgatacact gcaattctca aatcacagaa tatgaaggat aagcttatgc
     1981 caggtctcaa tgtccagttg gatgcgcttc gtcaatctcc tacaatcgcg atgggtgacc
     2041 gcgtcatttc actgaaggcc agtgggcgag acattattgg cctacaggtg ggagacccgg
     2101 actttgcgac acctcaaccg gtcattgatg tagcgatgca ggccttgcag gagggcttga
     2161 ctcattacgg gccaagccgc ggctatttgg accttcggca ggctgccgct tccaagctac
     2221 atcgagacaa tgatctttcc tatgatccgc aacgtgaaat cctggtaacg catggaggca
     2281 tccatgcgta ttatcttgcg atgcaatcca ttttgaatcc gggcgaggat gttttgatcc
     2341 cagaccccag ttggggaacg catagcaata tggcagtgat gctgcgttgt aatgtgatac
     2401 gagtccctgc acccgcagag aatggattca tcccatattt tgaaacctgg gaaaaagtgc
     2461 taactccaaa gacgcgcgtg attgtgctga attatccatc gaacccgacg ggcgcttacc
     2521 cgacacgcca gtatcttcaa caattgcagg actttgccga gaaacacaac ctgtggatca
     2581 tcagcgatgc agtttatgaa agcctgtatt acgaagaaaa accaaccagc gcagcatcct
     2641 tcgaaggagc aaaggatcgt acgatccttg tcaacagtct atccaaaacg tatgcaatga
     2701 cgggttggcg ggtaggtttc ctggctgcac cacagcaggt gatcaataac gcagtgaagg
     2761 cagggcaaaa ttccattact tgcgtcgcac cattcatcca aaaagcggct gcatttgctc
     2821 ttaccgaccc aggcgttcaa caaaacgtgg aagatatgcg gcttgcctac gcgggtcgac
     2881 gtgatttggt catgcgactt tctcatgagt tagagagtga aaaggttttg gtcaagccgc
     2941 cgcagggcgc gttctatttc tttctcgatc ttcgtccatt gaagatgacc tccctcgaaa
     3001 tctgtgagcg gattctggaa gaggtcggtg tggggctggt ccctggagca gcgttcggcg
     3061 agcaggggga gggcttcgtc cgcatgacaa ttgcagcttc agacgaggac gtggaaacag
     3121 gatttagaaa gattgtggag tgggcggaga ggcaatgaca tatctggagc aatatgcgag
     3181 ttgcatttca aggtgagccg ggtgcgtata gtgaacaggc tgtgttcaat tatttcagca
     3241 gtgtagaaac acagccatgc gaatcattcg atacggtttt tgatgccgtc gtttctaatg
     3301 cgtgtgacgc ggggctgatt ccaattgaga attcactcgc aggtagtatt catcagaatt
     3361 atgacctgct cctgcatcac gaccttcata ttaccgggga atatctatta agagtacaac
     3421 actgcctgat cgcacaacct gccgtaaaga agcatgaaat tacaaaagtc atcagtcatc
     3481 cccaggcgct gggacagtgc gccgcttatc tacggaatct gggagttaaa gtcgaagccg
     3541 tctatgacac agcgggaagt gtgaagatgt tgaaagagtc aggcatgcgt gatatggctg
     3601 cgatcgcatc gcgccgtgcg gcggaaatct atgggatgga gatcctcgaa gaaggcattg
     3661 aagataaccc ggaaaactac acacgtttcc ttgcgatttc aaaaaatgca gtgaaaccag
     3721 agggtgaatc gaaaacatcc atcgtgttca cattgaaaaa ccaacccggc gcgttgttca
     3781 aggccttgag tgtctttgct ctgcgagata ttgatttgac caagatcgag tcacgccccc
     3841 tgaagggcca gccctgggaa tatctgtttt atattgattt catcggcgca gctcatgaag
     3901 aaactgccag taaagcactg gatcatttga atgaatatgc gttgatgctg cgggtattgg
     3961 ggtcgtatgc gaggtataag taaatagact tcgtttgctt tataattttc ctatgattac
     4021 gagtgcagga ggataatcga ttgttaaata cgatccgtcc ttacattact ggagatgaac
     4081 cgtttgggcc aattgattct gtcgatatcg attcaggcga cattcaagcc ctgaaattac
     4141 tctttgaaca acataactgg atttataaga atctgcgcaa ccggccatct attattattg
     4201 ggcggagagg atctgggaaa acctattatt tgcgaagtgt gttctttgat aagcaatacg
     4261 atttttatgt agagatccgt acagcgcgtg tactggggta tatcgccagg gtgatccaga
     4321 aaatgacgaa agaagtcgtc ttccccgaaa tggtttctga gctttgggag accattttgt
     4381 ggatcgctgt gttttcggag ctccgaaaac attcggtgat cgcctcggaa gatttgaatc
     4441 tgatcaatgc ctatctggac aagatgagcg tgctcgacaa cgataatgtg gaggacgtgc
     4501 tttggaacgt cgcggccatg ctggatgaga tcatgaataa gaatccaaag acaatggcat
     4561 ccgacgtcct gcgaggatac aaccggatca cgattgatca cgcaaaagct gcggtcgtga
     4621 gaaatcttga atcgtcaaaa aagaattttg tcatcctgat ggattcgctg gacgatttcc
     4681 acctggatat cgattcggtg gcgcggtccc ttcagggact tctcaaggtc gtcggcgcga
     4741 tgaacaagcc aagagacgta gtagacatcc gcttctgcct gccatcggag gtctatcacc
     4801 gtttcatcaa aatttcctcc aacccgaaca aagatttccg gcgcgcgctc aagcttcagt
     4861 ggacggcggc ggaattgatc ctgatcgctg cccagcgact gatgttgtac ctgatgttgt
     4921 accacccgga ctttgtaaag acgatccttc cactcgagcc cactcggcgg gcagatgcct
     4981 taaagctttt tcaagccgta ttccctgaaa agatcacgaa ccagagcggt tttcaggaag
     5041 agacgatttc ctacatcctg cgccatactc agcttttacc gcggcatttt ttgatgttac
     5101 taaattcgat cttcaagggt tccaatactg cacagcgcaa aaatccattc ccgatcagcc
     5161 aaaatcgaat cgtcagtggc attcgtcaaa tggaagaatt catggtcggc gaaatctttg
     5221 tcgccttcaa attgaactat cccaccgccg aagagacctg cagacgttgt ttgccagagc
     5281 tcgggcacaa atttacacag gcagatcttc ataaagtgtt tacacgtcat ggaaaagccg
     5341 tcttcggtag cgacaacctg ttcgaatttc agcgaatgtt gctggagatc ggggcattgg
     5401 gcagggtgat cccgggtaag gagaatgatg tttatatcaa aggttatttc gaatataccg
     5461 ttgcacacga actggtctta agccaggacg atgagttgtg cattcatccg ctcttttccg
     5521 gcatcttcca cagaggggga aaggatcgtc cagtctatcc gtacggaagt gtgttggatg
     5581 atgaagatta tcgcgatagt tttgagtagt tgttaaaatg aatgagagat ctcatcaaga
     5641 gacctctcat ttcttttcaa gcttataaga gcggattttt cagggcgtag atgttggcgg
     5701 cgtccccaac ggattttgga caggcgcacc gggattggca ccctggtaat aggggaaagc
     5761 cacaggcagt tcgaaaccga aatactcaga tacacgcgcg accacttccc cggggatttg
     5821 tgccctgcgg gtgttatacg cctgcacggc atcattccaa tctgtgccag ccaattgaat
     5881 cgtattcatt ccctcgtcaa tcgtatcgcc aagcgccaga gtaacttccg gagctggcac
     5941 aggaggcggt gcctgcacaa ccgcgttgac aaagagggta aaaccttcac ccaagctatc
     6001 aggtaatgca cccttgttct gcagatcagc caggttcaac tgggtcggat caagcggctg
     6061 accgttttca tcacgatagt tcctcaacaa ctcattcagt aattcctgct ccgaacggaa
     6121 gttgccggtc catacttcac cactattgct aaccgcatcg taatagtctt tggtgatttg
     6181 cgttttggct tgaatctttg catactgtgt atccgtcacc aattgaaaat tagaggcagc
     6241 ggcctgaaca ccctgcacca gcgccaccag gtcgttgtat tcgtccgcgc cagattggat
     6301 gagattacaa ctggctaatg agacaacaat aacgagcgtg gcaaaaatgg aaaaagtttg
     6361 tttaatggaa tgtttcatcg tttagctcct tattttttag ttcgtgcgtc ccgagcgacc
     6421 acttccgctt ccaccgcgca tgcgcggact cggaaagcct ctgctcccac ttgatgggaa
     6481 ccctctggag gggaaacttc gccagggact tccgccccag gaactccctc cccatggatt
     6541 gctttgccac gggttggatt ggggagtgta ataccttctc ccgcctccac ccagctggct
     6601 gaacatccat gctagaaata caaataacag aaggccaaga cagaactgac cgcagagggc
     6661 cactagcgcg gctggtccga caggctgatc ggtgacctct ggagtcacta ctgcggaagg
     6721 cgcaggatca tagttactgc gtgccgccgt gctgaactca cttgccagag tcaacagggc
     6781 ttgatccata ctatgtgtcg actgatacga atcttcaact gcgcgattga tgttcgtcaa
     6841 ttcaggcgca gtcagtgctg gcaaccccag ccctaccgcg tagtgcacat caatatcatc
     6901 gggttcaaca acgattaaaa agacaaaacc gttatctttg cgtgcacccg ttggcaagcc
     6961 aagttccatg taacgcagaa aatggaccgc gcaattggtc cgaatgccca cctgatcctg
     7021 cggcaggata agaatcatcg tctgcgcaat tgcatcttca tccagccggt caaagaccct
     7081 gtcaactcga tcgacagcct gctctgtggc gtaaccttcc cagcctactg tccaatgtgg
     7141 ttgcagtaga gaataggaga ggccataatc aggaggggaa ccattgccac acagttcatc
     7201 agtagtctga gcccggactg gagtggtgta ccccaacgcg gaaagggcca gcgccacgag
     7261 ggcaaccagc ttatacgctt gatatgccag attcgccccc ataaaacgtc ttagtaaatc
     7321 tggatttctg ctatgtgtgt gcaatgtgtt tgtcctgcga agaactttct tcgatcaatt
     7381 atcaaaagta tattatcggg aaatgatgac gtggacaata aggaatctcc ctaatatgga
     7441 aatttcctct gctccatcca taatggctgg gatatcagtt cataattcgt tattgcccga
     7501 attcttttag aaggtaactg ttttactgta tactcccaac taacattaat catggctttt
     7561 ctttatgaag aattcgagat tcatacagat ctcgatacaa gtaatgttta caatagggtt
     7621 ttgtcagttg ttgaaaccaa gcagttttgg gcttcaccac tttcaacagc caaaccatat
     7681 tttggcgaag tgcaaagtac ttcctttgat attaggaggg taaagtggaa tagcagagct
     7741 attaggcctg ttgttgttgg aaaaatttca tcaaataata aaggtagtta tctgcgtgtt
     7801 cgagtacggc taaattggtt cgaattcgtt ttttatatgt tagtttttgg cttgttcgga
     7861 cattttgcat tctggtattt tatcgaaaat cttagtgctg acactttggg ctggctcggt
     7921 agaaccttgt ctttcttcat tcctcttaca tcttttagca ttgtttattt gctaattttt
     7981 atttctgtga aagttgaggc gatttccgcc aaacaatttt ttctcgaatt gctgaataaa
     8041 aattcaaacg tagaattccc ttgacctgtc ttgccaatgg ctactatata tgcctcttga
     8101 caacctcccc cctccgtcat acaatcccgc ccaacaacat aataaaacgc tgacgaggac
     8161 gagtacgttt caaagcgatt ttcagagagc caagccgttg gtcgagtacg ccgaaggcgt
     8221 gtatcgagac cagagtgatg agagcaaggc aaaagcgcag aagccgaatg gacctccgag
     8281 ttgcgaagtg aaagatacta gagagtagta ttaagtaacg gagacgggaa ctgcacccgt
     8341 taccccgcag aggcacttag tgactagaag tcgaaccgac tagcccacta gctgactaaa
     8401 aagagtgacg ctggcttgaa ttctctgtat cacacaggga attttttatt aaacagaaaa
     8461 agcgttaact tgggtggcac cacgagactc ccctctcgtc ccaatggaca ggaggggatt
     8521 ttaatttaag atattcgatc atcgatattc ggttttcgag tgtccaatat cgaatatcgt
     8581 ttggagatta atatgttact cgaagctacc acagtcaaaa cgatactgca aagtgtcatc
     8641 cgcgagatca acgccgacct cgaaacacct gtcagcctgt acctcaagct gcgcggggag
     8701 ggcgcatcct ttttactgga atccgtcgaa ggtggagaac ggatcgcgcg ctattccttt
     8761 atcggagtcc agccgcgggc ggagtacatc ctgcgggatg ggcaggtgga ggtcaaggag
     8821 aacggcggga cccgtaccct gcagctcaat gacgacccga cgcgcttcct gcaggaggag
     8881 atgagtcgct tctccacaga gcgcctgccg aacgccccgc gttttacggg tggactggtg
     8941 ggctatctcg ggtatgagtc ggtccgttat tttgagacga cgttaaagtc ccaattaact
     9001 cgcgctgagc ggagtgagcc gtcaggcgaa cgaagtcgaa gcgtcccgga tggcatctac
     9061 ctgctcgcgg ataccgttgt cgccttcgac cacgcccgcc gcagcatctt cctgatcgcc
     9121 aatgttctgg atggggacct ggaagccgca aaccagaagc tcgatcagat cgccgagcgc
     9181 attgcccaac cacttccagc agcaggcaag acaaccgtca cgccttctga gatccgctcg
     9241 aacatgacgc aggaggctta cgaaaccatc gtctctgagg cgaaggagca tatcgccgcg
     9301 ggcgacatct tccaggtggt gctctcccag cgttttac
//
LOCUS       DASTJP010000649         8253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_281556, whole genome shotgun sequence.
ACCESSION   DASTJP010000649 DASTJP010000000
VERSION     DASTJP010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8253
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281556"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="VFQ23_19040"
     CDS             <1..1899
                     /locus_tag="VFQ23_19040"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9908753.1"
                     /translation="IVIFVGAALWDLWLYATPGGLTLRAVGHDAQAARRIGAATTRIR
                     VGAFVLSGLMAAVASFFLAAQVGIGDPRAGTSYALSSLAAAVLGGSSLMGGRGTFIGA
                     IVSALFLTLIINILPLLGLSNVVGLISVGVLLLLGLAFYQINDLKELIKLNFKRVQRL
                     VRGSRLNQLRDLPKVYVSDATTFPATEKQVLLKGGTVLTMDPALGNFRTADVLIEGTK
                     IAAVGPNLAANGAEVIDASNMIVMPGFVDTHRHIWEGLLRNVGADTPLEGREGYIRFV
                     LGKFAPSFRPQDAYVGNLVSALGAMDAGITTLLDWSHIQGSPEHTDAVIKALQDSGMR
                     AVFAYGFPWWGDWNDRQPSWFVRAAKEYFSSKDQMLTYALAAPGPEFVDFEIARDHWK
                     LAREVDARITTHVGVGSYGQEGKVQEMGEAGLLREDTTYIHCTTLNETEIQMIVDSGG
                     TVSLAAPVEMMMGHGMPPIQKFLDRGLKPSLSVDVETNVPGDMFTQMRSVMSLQHALS
                     YERQLIGEGKAPERITTRDVLAYATIEGARANGLAHKTGSLTPGKEADVIMLRTDKPN
                     VFPINDPIGAVVWGMDTSNVDSVFVSGKAMKRNGELLHVDWNAMKKTVTESRDYVMKK
                     SGFKLPPI"
     gene            complement(1933..2922)
                     /locus_tag="VFQ23_19045"
     CDS             complement(1933..2922)
                     /locus_tag="VFQ23_19045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908754.1"
                     /translation="MTSTAIPKSIGRKDIAESMVESPWRLAWRRFRRHRAAVTALAGI
                     FVILAYIWFGGLFFSRGLCAPTGEYLTSEAYANCNDLAKKLQPPSREHPFGTDTIGRD
                     ILARTIYGGQISLSIGIFAAIVEVFVGVLVGAIAGYFGGWIDDLLMRITEAMLIIPSL
                     FLLLVLSKMLGGRVPPIELLGRTFSGSVIVIILVIGFTSWMYLARIVRANVLSLKNLD
                     FISASRALGVGDARIIFRHLLPNTIAPIIVSSTLGVSAAILLESYVSFLGLGVQTPTA
                     SWGNMLDTAVKYVQTAPWLWFFPGLFILLTVLCINFIGDGLRDALDPRSTKHV"
     gene            complement(2950..3981)
                     /locus_tag="VFQ23_19050"
     CDS             complement(2950..3981)
                     /locus_tag="VFQ23_19050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908755.1"
                     /translation="MLNYIIRRLLQVIPQLFIISLVLFVLMQAFGDPIASLGVRTPPR
                     PEDRVRLRRQLGLDQPIYVQYMVWLVGNDWMKIDMDGDGVGETPGRRLGVFRGDLGNS
                     LINKRPVIELITERLPNTLLLMITVEIVIIVISLLLGVYSAVRQYTLFDNLVTGLSFI
                     GYSIPIPLIALLLMYLFSVNFKEWGLPHLPTVGMFDPQVGRTPGQIALHMVLPVISLS
                     IISIAGYSRYVRSSMLEVLGLDYVRTARAKGLRQNVVVMRHALRNATLPFVTLIGLDI
                     PFLLAGAVLTETIFAWPGTGRLFITHLTQSDFPVLMGILMMTGIAVVIFQLITDITYS
                     FLDPRIRIE"
     gene            complement(4154..5878)
                     /locus_tag="VFQ23_19055"
     CDS             complement(4154..5878)
                     /locus_tag="VFQ23_19055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9908756.1"
                     /translation="MKSSKLYLILSLVVVASLTLAACGGSPAATDAPVTEEPAAPAAT
                     DAPAGTEAPVATEPPAATGGIAVVNFVQQPTTLSPLYQTQWFSSITTQFWLKSLWSFD
                     PDNNPVPEIATEIPAPSEDGLTLTINLRDDVTWSDGEPVTANDFVFTYEMFMAESNIV
                     ASRYPYEDYVASVEAADDQTLVVTFTEPFAAWLTSLFTTGVLPEHVLRPVFDADGTLD
                     TAEWNTAPTVGVGPFVFSEWETDSHMILEANPNWINPPQLEQVFIRFADDAAQEVAIL
                     AGDADIGTFLDWSQADAINNSGVAQFVTQPSGYDEGWFLNFDPATAHPAMLDVNVRKA
                     IVHATDREKIVQDLLDGLTEVPVTFWDSTPPFGNPNLTPYAYDPEEANRLLDEAGWVD
                     SNGDGTRDKDGTELVLRYIANQRQLRKDVQAVVQQMWAQVGIGTEIVNYGDDYFNGYA
                     DGGPQAIGNYDIAEYSSTGSYPDPEASFNWLCSEVVGAENPDGGNWQGYCNEELDALF
                     DQQATTTDPTARQELYYQIGEIMHNDVVWVGIWKDPDLWALSTRLQNVRFSGATPFWN
                     AHEWTISE"
     gene            complement(6182..7141)
                     /locus_tag="VFQ23_19060"
     CDS             complement(6182..7141)
                     /locus_tag="VFQ23_19060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908757.1"
                     /translation="MSTATGTTLDIPVLEREASRPILTPRQLIWRRFRKHRMALIGMI
                     GFLFLLAFIIVGSILISEKEANEVDLQARLSPPSAAHWMGTDSTGRDLFARIIHGGQI
                     SLIVGVLAVILSVTVGTLIGGIAAYYGGWVDSLLMRFTEAMLSIPSLFLLIILGKFFG
                     KDVQTIEVLGRTFSGSVGIVILVIGLTSWMYLARIVRANVLSLRELDYISVSKSLGAT
                     DMRIFFNHLIPNTMGAIIVSSTLGLAGAILSEAYVSFLGLGVQPPTASWGNILTSAQS
                     LIQRGSWWVWLFPGTFIIVTILSINLVGDGLRDAFDPRSTRHL"
     gene            complement(7238..8197)
                     /locus_tag="VFQ23_19065"
     CDS             complement(7238..8197)
                     /locus_tag="VFQ23_19065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908758.1"
                     /translation="MWSYLARRVVQASFTLITITVLCFLLTRFSGDPMAQYAANPRMS
                     AEDRAALRERLGLNQPLPVQYLKWLGLALQGDLGQSFFSKQPVTEMISTRLPNTLVLM
                     LTAEIVTVGLALLLGILAAVKQYSALDNIITSFSFIGFSMPIFFIALGLMLIFAVQFK
                     EWGLPYLPTGADIWDKSDPVELATHLVLPVAALAIVNTAGYARFVRTSMLEVLGLDYV
                     RTARAKGLTSREVMFKHALRNAALPLVTIVGLDIPFLLGGALVTESVYAWPGMGRLFW
                     EYAQRGDYPVVLGVLLIISTAVVVFTILTDLIYTFVDPRIRLS"
BASE COUNT         2094 a         2109 c         2181 g         1869 t
ORIGIN      
        1 attgttattt tcgttggcgc cgctctctgg gacttgtggc tgtatgccac tcccggtggg
       61 ctcaccctcc gcgcagtcgg ccatgacgcg caggcagcac ggcgcattgg ggcagcaacc
      121 acgcgcattc gcgtgggcgc ttttgtactc tcaggcttga tggcggccgt ggcttccttc
      181 tttctcgcag ctcaggtagg catcggtgat ccgcgcgcgg gaactagtta tgccttgtcc
      241 agcctggcgg cggctgtgtt gggcggctcc agcctgatgg gtggtcgggg tacatttatc
      301 ggcgcgattg tctcggcttt atttctcact ttaattatca atattctgcc tctgctgggc
      361 ttgagcaacg ttgttggttt gatctccgtt ggcgtgcttt tattgcttgg actggcgttt
      421 tatcaaatca atgatctaaa agagctaata aaactcaatt tcaaacgcgt gcaacgattg
      481 gttcgaggtt cgcgcctgaa tcaactgcgt gatctgccaa aggtttatgt aagtgatgcc
      541 acaacgtttc cagcaactga gaaacaggtt ttacttaaag gtggcacggt tctcacgatg
      601 gacccggccc tgggaaattt tagaacggct gatgtactga tcgaaggcac gaagattgcg
      661 gcagtcggtc caaatttagc tgccaacggc gcagaagtga tcgatgcttc caacatgatc
      721 gtcatgcccg gcttcgtgga tacacaccgt catatctggg aaggactgct ccgcaatgtc
      781 ggtgctgata ctccactcga gggacgcgaa gggtatatcc gtttcgtgct tggaaaattc
      841 gccccatctt ttcgaccgca ggatgcctat gtagggaatc tcgtcagtgc actgggtgca
      901 atggacgcgg ggatcacaac attactggat tggtctcata ttcagggatc tcctgaacat
      961 accgatgcgg tgatcaaagc tttacaagac tcaggcatgc gcgcggtttt tgcctatggc
     1021 ttcccctggt ggggcgattg gaatgaccgc cagcccagtt ggttcgtccg tgctgccaag
     1081 gaatacttct cctccaaaga ccagatgttg acgtatgcat tagccgcacc cggacccgag
     1141 tttgtcgatt ttgaaatcgc acgcgatcac tggaagctgg cacgcgaagt ggacgcacgc
     1201 atcaccacgc acgttggtgt aggttcttat gggcaggaag ggaaagtgca ggaaatgggc
     1261 gaggcagggt tattacgtga agacaccacc tacatccatt gcaccaccct aaacgaaact
     1321 gaaattcaaa tgatcgttga ttcgggtggc acagtctctc tggcagcgcc agtggaaatg
     1381 atgatggggc acggcatgcc gccgattcag aagttcttgg atcgcggctt gaagcccagt
     1441 ttgagtgttg acgtggaaac gaatgtgccc ggcgacatgt tcacgcagat gcgttcggtc
     1501 atgtctttac agcatgctct ttcttatgaa aggcaattga ttggagaagg caaagcgccg
     1561 gagcgaatca ccacccgcga tgtactggct tatgcaacta tcgaaggcgc gcgtgcgaac
     1621 ggattggctc acaaaacagg ttcactcacg cctggcaaag aagcagacgt catcatgttg
     1681 cgaacggata agcccaatgt cttcccgatc aatgacccga ttggcgcggt ggtctggggt
     1741 atggatacca gcaatgtcga ttcagtgttt gtatccggga aggcaatgaa gcgcaatggt
     1801 gaattattgc acgtcgattg gaatgcaatg aaaaagacag tgaccgagtc tcgggattat
     1861 gtgatgaaga agtcggggtt taagttaccg cctatctaaa aaaatacgga cggcaacagc
     1921 cgtccgtttt atctacacat gcttcgtgct gcgcgggtca agggcatcac gcaagccatc
     1981 cccaataaag ttgatacata atactgttag caaaataaat aagccgggga agaaccagag
     2041 ccaaggcgcg gtctgcacgt actttactgc ggtatcgagc atattgcccc aactggcagt
     2101 aggtgtttgc actcccagtc caagaaaact gacatatgac tcaagcagga tcgcggcaga
     2161 aactccgagg gtgctggaga caatgattgg agcaatggta ttcggcaaca agtgccggaa
     2221 gatgatgcgg gcatctccaa cgcccagcgc gcgtgacgct gaaataaaat ccagattttt
     2281 aagagaaagc acattggcgc gcacgatccg cgccagatac atccaactgg tgaaccctat
     2341 caccaggatg ataacaatca cactcccgct gaacgtccgc cctaacagtt caattggggg
     2401 cactctgcca cccagcattt tagacagtac taggagcagg aacaggcttg ggatgatcaa
     2461 catcgcttca gtaatgcgca tcagtaaatc gtcaatccaa ccgccaaaat aaccagcgat
     2521 cgcgcccacc agaaccccaa caaacacttc tacaattgct gcgaagatac caatggacaa
     2581 tgaaatttgc ccgccataga tcgtgcgcgc caggatatca cgcccaatgg tgtctgtgcc
     2641 aaatgggtgt tcacgcgaag ggggctggag tttttttgcc agatcgttgc agttggcgta
     2701 ggcctctgat gtcaaatatt cgcccgtggg cgcgcacaaa cctctggaga agaacagccc
     2761 tccaaaccag atgtacgcca gtattacgaa tatccctgcc agtgccgtca ctgctgctct
     2821 atggcgacgg aatcgtctcc acgccagacg ccaggggctt tcgaccatgg actctgctat
     2881 gtctttgcgc cctatcgatt ttgggattgc tgtcgacgtc attggtccac tctccgctga
     2941 gtcagagggt tattcgatcc gaatacgcgg gtcgaggaaa gaatatgtga tatccgtgat
     3001 gagttggaag atcactacgg caataccggt catcatcagg atgcccatca aaacggggaa
     3061 atcagactga gtgaggtgtg tgataaagag acgcccggtg cccggccagg caaagatggt
     3121 ttctgtcaac actgcgcctg ccagcagaaa ggggatatcc aggccgatca gagtgacgaa
     3181 cggaagtgtt gcattgcgca gggcatgacg catgaccacc acattctgac gtaatccctt
     3241 ggcacgcgcc gtacgcacat aatccaaacc aagcacctct aacatggagg accgcacgta
     3301 acggctatag cctgcaatgc tgatgatcga gagtgagatc acaggcagaa ccatatgtaa
     3361 cgcgatctgc ccgggcgtgc gcccaacttg cgggtcaaac atccccaccg tgggtaggtg
     3421 tggcaacccc cattccttga agttgacgct gaataaatac atcaataaaa gagcaattaa
     3481 aggtatggga atcgaatacc ctataaacga aagaccagtt acaagattat cgaagagtgt
     3541 atattgcctc actgcagaat atacccccag caaaagtgag atcacaataa taacgatttc
     3601 caccgtgatc atgagcagga gggtattcgg caaacgttcg gtgatcaatt caatcaccgg
     3661 tcgtttattg atcagagagt tgccaagatc accgcgaaaa actcccagcc ttcttccggg
     3721 tgtttcaccg acgccatcac catccatgtc aatcttcatc cagtcattgc ccaccagcca
     3781 gaccatgtat tgaacgtaaa ttggttggtc caaccccaac tggcggcgaa gccgtactcg
     3841 atcttcgggc ctgggtggcg tgcgtacacc cagggacgca atcgggtcac caaacgcctg
     3901 catcagcaca aacaagacga ggctgatgat gaataattgg ggaatcacct ggagcagacg
     3961 ccgaatgatg taattgagca tgaaatggat aacctttatg tcattgcgag gagcgtttgc
     4021 tgcgacgaag caatcttcta gttaacgaga taatctacgt atgtaagtat ctatcgttta
     4081 gaaagagatt gccacgtcgc ttcccttcgt cttcgctcag ggtcgctcct cgcaatgaca
     4141 tttttaacgt gacttattca ctaatcgtcc attcgtgcgc gttccagaag ggggtcgcgc
     4201 cggagaatct tacattctgc agacgagtac tcaatgccca gagatccggg tctttccaga
     4261 taccgaccca gaccacatca ttgtgcatga tctctccgat ttgatagtag agctcctggc
     4321 gagctgtggg gtcggttgtg gttgcttgct ggtcaaacag agcgtccagt tcctcgttgc
     4381 agtagccctg ccagttaccg ccatccgggt tctctgcacc gaccacctca gaacacaacc
     4441 agttgaagga agcttccggg tcagggtaac taccggtgct ggagtactcg gcaatatcgt
     4501 aattgccaat cgcctgaggg ccaccatctg cataaccgtt gaagtagtca tcgccatagt
     4561 tcacgatctc cgtgccaatg ccgacctgtg cccacatctg ctgtacgacg gcttgcacgt
     4621 ctttgcgtaa ctgtcgttgg ttggcaatat agcgcaagac cagttcagtt ccatctttat
     4681 cgcgagtgcc atctccattg gaatccaccc agcccgcttc atccagaagg cgattggctt
     4741 cctctggatc atatgcatag ggcgtcaaat tcggatttcc aaagggaggt gtgctatccc
     4801 agaatgtaac cgggacttcg gtcagcccat ccagcagatc ctgaacgatt ttctcgcggt
     4861 ccgtggcatg gacgatggct ttgcgcacgt tcacatccag catggcggga tgtgcggttg
     4921 ccggatcaaa gtttaagaac caaccctcat catagccaga aggctgagtc acgaattgtg
     4981 ccacaccact gttgttgatc gcgtcggcct gagaccagtc aaggaaggtg ccgatgtccg
     5041 cgtccccagc aagaatggca acttcctgcg ctgcgtcatc tgcgaagcgg atgaagacct
     5101 gctccagttg aggtgggttg atccaattgg ggtttgcctc aaggatcatg tggctatcgg
     5161 tttcccactc gctgaacaca aatggaccta cgcccacggt gggggccgtg ttccattctg
     5221 cagtgtccag cgtgccatct gcatcgaaca cggggcgaag cacatgttca ggaagcacac
     5281 cggttgtgaa gagtgatgtt agccaggctg cgaacggttc agtgaatgta acaacgagag
     5341 tctgatcatc ggcggcttcg acacttgcca cataatcctc atagggatag cggctggcaa
     5401 cgatattgga ttccgccata aacatttcgt aggtgaagac gaagtcatta gctgtcactg
     5461 gctcaccatc actccaggtc acatcatcgc ggagattgat tgtcagtgtc agtccatcct
     5521 cgcttggcgc aggaatttct gtggcgatct ccggaaccgg attgttatcc ggatcgaatg
     5581 accaaaggct tttaagccaa aactgtgtgg taatactgga gaaccattgg gtttgataga
     5641 gagggcttaa tgtagtgggc tgttgcacaa agttaaccac ggcgatacca cccgtagccg
     5701 cgggcggttc ggttgcaaca ggtgcttcgg taccggcggg ggcatccgtg gcagctggcg
     5761 ctgcgggttc ttcggttacg ggtgcatcgg ttgctgccgg gctcccgccg caggctgcca
     5821 gcgtgaggct tgctactact acgagcgaaa gtattaaata tagtttgctg gatttcacgt
     5881 ttctcctcct gagagaatct attgaatttc aacacggtgg gaaggggatt gattgggtgg
     5941 tcttcgtcgc gagacctcct ttcaaattaa cgttaccgct tgtttactaa acctttgaaa
     6001 ttgttgtagg ctaaaaatca agcgtccatt agctggttaa gctaagcgat gatacttgaa
     6061 aaattataca gcaaattact tccttgcaca gacgagatta aaccccaata ttaaatcccc
     6121 ttatctgcgc atcgtttgct acataaattt cgagccgttt gtaaaaaaac tgttatatct
     6181 cttacaaatg tcgagtgctt ctggggtcga aggcgtctct gagaccatcg ccgaccaggt
     6241 tgatggacag aatggtgacg atgatgaagg tgcccggaaa caaccagacc caccacgacc
     6301 cacgctggat caacgactgc gcactggtca ggatgttgcc ccaggatgcg gtcggtggtt
     6361 gaacacccaa tcccaaaaac gaaacatagg cttcagaaag aatcgctccc gccagtccca
     6421 gggtggagga gacaatgatg gcacccatgg tgttggggat caggtggttg aagaagatgc
     6481 gcatgtcggt ggctcccagc gacttcgaca ccgagatgta atccaactct ctgagggaga
     6541 ggacattggc gcgcacgatg cgagccagat acatccagga ggtgagaccg atcacgagga
     6601 tgacaattcc cacgctgcca ctgaaggtac gacccaggac ttcgatggtt tgcacatcct
     6661 tgccaaagaa cttgccgagg atgatcaaca gaaaaaggga agggatggag agcatcgctt
     6721 cggtgaaacg catcaataac gagtccaccc agccaccata gtaggcggcg atgccaccga
     6781 tcagggttcc cacggtgacg gagaggatga cggccagcac accgacgatc agggagatct
     6841 gacccccgtg aataatgcga gcaaacagat cgcgaccggt actgtcagtt cccatccaat
     6901 gcgcagcaga tggcgggctg agccgtgcct gcagatcgac ttcgttagct tctttctcgc
     6961 tgatgagaat ggaccccacg atgatgaatg caagcaggaa cagaaagccg atcatgccga
     7021 tgagggccat gcgatgtttg cggaagcgtc gccagattaa ctgacgaggg gtcaagatcg
     7081 gtcgtgaagc ttcgcgttcc aatacaggaa tgtctaatgt cgtcccggtg gctgttgaca
     7141 tgttcttact cctttacctc aacatgaata gctgtttaat cagaaatgcc aatgttattc
     7201 aggtcgaaat gcatccaata acaaattcga ggttcaatta actcaatcga atacgagggt
     7261 ctacaaaggt ataaatcaaa tcggtgagga tcgtgaacac caccacagcc gtggagatga
     7321 tcaataatac tcccagcacc accgggtaat cgccgcgttg cgcatactcc cagaacagac
     7381 gtcccatccc tggccaggca taaacgcttt ctgtcaccaa cgcccctccg aggagaaatg
     7441 gaatatccaa accgacgatg gtcacgaggg gtaaggcggc gttgcggagg gcatgcttga
     7501 acatgacctc acggctggtg agtcctttcg cacgtgcggt gcgaacatag tccaacccca
     7561 gcacttccaa catgctggtg cgcacgaaac gagcataccc cgctgtattg acgatggcga
     7621 gcgcagccac aggtaaaacg agatgcgttg ccagctcgac cggatcactc ttatcccaga
     7681 tatccgcgcc tgttgggaga taaggcaagc cccattcctt gaattgcacg gcaaagatca
     7741 acatcagtcc caatgcaatg aaaaagattg gcatcgaaaa gccaatgaaa gagaatgagg
     7801 taatgatgtt atccaaagcg gagtactgtt taacggctgc tagaattccg agcaatagcg
     7861 ccagccctac tgtcactatc tctgcagtca gcatgagcac cagagtgttc ggcagacgag
     7921 tagatatcat ttcggtgaca ggctgtttgg agaaaaagga ctgtcccagg tccccttgta
     7981 acgcaagacc cagccacttc aaatattgga ccggcagagg ttgattcagt cccaagcgtt
     8041 cgcgcagggc ggcccgatct tcggcagaca tacgcgggtt agcagcatac tgagccatgg
     8101 ggtctccgga aaaacgcgtc aggaggaaac acaacacagt aatcgtgatc agagtgaaac
     8161 ttgcctgaac tactcttcgc gctaaatagg accacataag acacctccgt tatccatctg
     8221 aggatgacga ccctgctttt gtaaaacttc taa
//
LOCUS       DASTJP010000650         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_817752, whole genome shotgun sequence.
ACCESSION   DASTJP010000650 DASTJP010000000
VERSION     DASTJP010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817752"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /gene="glgP"
                     /locus_tag="VFQ23_19070"
     CDS             <1..899
                     /gene="glgP"
                     /locus_tag="VFQ23_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET9908759.1"
                     /translation="NEYIAEDWLDRLDEPEIWENIDDVPDEGLWEVRQHLKRKLVFYM
                     RERVRDRWTVGGFHPVQVVSSGVLINPYALTIGFARRFATYKRASLVLSDVERLLKII
                     NRPNMPVQIIFAGKAHPADDPGKQLIQQVYRQVKRAETGGRIVFLEDYDMNLARYLVQ
                     GVDVWMNTPRRPLEASGTSGMKAALNGALNFSVLDGWWRESYNGENGWAIGEDKEMES
                     QSVQDAQDAESLYSTLENEIIPLFYDRQQDEISRNWIARMKESLKKNIPQFSTRRMVK
                     EYVNKLYVNASKSSTPNERKKG"
     gene            921..1253
                     /locus_tag="VFQ23_19075"
     CDS             921..1253
                     /locus_tag="VFQ23_19075"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9908760.1"
                     /translation="MTVSELSEKLKSQDSFILLDVREPMELDMAEVTDSRLEVTPMSR
                     LAQEGINALSESAKSQDAVIYVMCHHGNRSAQVTAWLSQQGWKNVFNVRGGIHEYARS
                     IDNSIGFY"
     gene            complement(1288..2412)
                     /locus_tag="VFQ23_19080"
     CDS             complement(1288..2412)
                     /locus_tag="VFQ23_19080"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET9908761.1"
                     /translation="MSDSRVQKFAKVLVEHSARIVPGDRVLIEGTTAAEPLVRELYIQ
                     ILEKGGHPHPMISFPGMVPFIQDDLYLTYAQDSQLDFVPTFYKLAYDQFESRIRIHSA
                     TNTRATTRIDPVKAQRRGKATSIITETQMRRGAESAFKWVTTLYPTDAYAQDAGMSLR
                     EYEEFVFGAVHAYEDDPISYWKSTAAGQQKAIDFLANKNQIVMRGPNVDLTLSIKGRK
                     FMNSTGIYNMPDGEIFTGPVEDSVNGWVKFTYPAIYGGVAVEGAELTFVRGRVEQAQA
                     EKNQEYLIKMLESDSGSRFLGEYAIGTNFDISKFTGNILFDEKIGGTFHMALGAGYPE
                     TGSHNKSAIHWDMICDLRTDSEILVDGELFYKNGEFVFGK"
     gene            2526..2999
                     /locus_tag="VFQ23_19085"
     CDS             2526..2999
                     /locus_tag="VFQ23_19085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9908762.1"
                     /translation="MTVQGLGRLKVWVRAKDFALKIYEQVLPQLPAEEKWNLNQQIRR
                     SSLSISANIAEGYGRFYCQDNVRFCYLARGSLEETLSHLVFAVEAKYIPVTLYEALAS
                     DGEEIDKMLNGYIAFLKKSKQGAKEPGGNYSLQEELTPFSPEPFESPTDIQEIDL"
     gene            3026..>4015
                     /locus_tag="VFQ23_19090"
     CDS             3026..>4015
                     /locus_tag="VFQ23_19090"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HET9908763.1"
                     /translation="MKQLLQNIKNGKSIVEDVPIPTPREGQALVKTEASLVSAGTERM
                     VVEFAEKSLVGKARSRPDLVKQVLDKARREGVLNTAQAAFNRLDQPMALGYSSAGTIV
                     ALGEKMEGFRVGQRVACSGGGYAVHAEYAVVPRNLLTPLPENVDFESGAFTTLGAIAL
                     HGFRLAEPQIGEAVAVIGLGLLGLLTAQIASAAGCNVLGIDIDAERIDLASSLGLDAV
                     PREKAIDSSAAFTNNRGFDVILICADTASDDPVELAGAIARDRARIVATGAVGLNIPR
                     KVYYEKEISFINSRSYGPGRYDSSYEEDGKDYPLGYVRWTEGRNFEAVIDLIAK"
BASE COUNT         1002 a          955 c         1041 g         1017 t
ORIGIN      
        1 tcaacgaata tatcgctgaa gattggctcg atcgtcttga cgagcctgag atctgggaga
       61 atattgacga tgtgccagac gaggggttgt gggaagtgcg tcagcacctc aagcgcaagc
      121 tcgtattcta catgcgtgag cgcgtccgtg accgctggac cgtgggcggc ttccatcccg
      181 tgcaagttgt ctcctccggt gtgttgatca acccatacgc attgacgatt ggatttgcgc
      241 gtcgttttgc gacgtacaag cgcgcaagcc tggttttatc tgatgttgag cgactattga
      301 agatcatcaa tcgtccgaac atgccggtgc aaatcatctt cgccggaaag gcacatcccg
      361 cggatgaccc tgggaagcag ttaattcagc aggtatatcg ccaagtcaag cgcgcggaaa
      421 cgggtgggcg cattgtcttc cttgaggatt acgatatgaa tctggcgcgc tatctcgtcc
      481 agggtgtgga cgtttggatg aacacaccac gccgcccatt ggaagccagc ggtacctctg
      541 gaatgaaagc cgcgttgaat ggagcgttga acttctctgt gttggatggc tggtggcgag
      601 aatcgtataa cggagagaat ggctgggcaa tcggcgaaga taaggaaatg gaatcccaaa
      661 gcgtccagga tgctcaggat gctgagagct tatactctac tctggaaaat gagatcattc
      721 ccctgtttta cgatcgccaa caggatgaga tttctcgcaa ctggattgct cgcatgaaag
      781 agtcgttgaa gaagaatatt ccgcaatttt ccacccgccg aatggtgaag gaatatgtga
      841 ataagctcta tgtgaacgcc tcgaaatcat ccactccgaa cgagagaaaa aagggatagt
      901 gcaaatctat gattccggaa gtgactgtaa gtgaactctc tgaaaaattg aaatcgcagg
      961 actccttcat cctcctggat gtgcgtgagc ccatggaatt agatatggct gaagttaccg
     1021 atagcaggct tgaagtcacg ccgatgagcc gtttggcgca ggaaggtatt aatgccttat
     1081 ctgagtctgc aaaatcacag gacgcagtta tctatgtgat gtgtcatcat gggaatcgca
     1141 gtgcccaggt aacggcatgg ctttcgcaac agggttggaa aaacgttttc aacgtgcgcg
     1201 gcggaataca tgaatatgcg cgcagcatcg ataattcaat tggattttat tagaatttgt
     1261 gtagggatgg gcagctgccc atccctacta ttttccaaac acaaactccc cgtttttata
     1321 aaacaattca ccatccacca gaatttcaga atcggtgcgc aaatcgcaga tcatatccca
     1381 atgaatagcg gatttgttat gtgaacccgt ttccggatag cccgcgccga gcgccatatg
     1441 gaatgtgccc ccgatttttt cgtcgaataa gatattcccg gtaaacttgc tgatatcgaa
     1501 attggttcca atggcgtact ctccgaggaa acgcgatccc gaatcggact caagcatctt
     1561 gatcagatac tcctgattct tctcggcttg cgcctgttcc acgcgtccac gcacaaacgt
     1621 cagctctgca ccttctacgg caaccccgcc atagattgcc ggataggtga atttcaccca
     1681 accgttcacg gagtcttcga caggtccggt aaagatttca ccatcgggca tgttatagat
     1741 acctgtagag ttcatgaact ttcggccctt gatggagagc gtcaggtcga cgttcgggcc
     1801 gcgcatcacg atttgatttt tgtttgccag aaagtcaatt gctttttgct gcccggctgc
     1861 ggtgcttttc cagtaggaga tcgggtcatc ctcgtatgca tgcacagccc cgaagacgaa
     1921 ttcttcatat tctctcagac tcatcccagc atcctgcgcg taggcatcgg ttggatagag
     1981 tgtggtgacc catttgaatg cgctttccgc gccacgtcgc atttgtgttt cagtgatgat
     2041 actggttgcc ttaccgcgtc gttgggcttt gactgggtca atgcgagttg tcgcgcgcgt
     2101 atttgtcgcc gaatgaatac ggatgcggct ctcgaactgg tcatacgcaa gcttgtaaaa
     2161 tgtgggaaca aaatcaagct gtgaatcttg tgcgtacgtc agatatagat catcctgaat
     2221 gaaggggacc atgcctggga acgaaatcat cgggtgcgga tgaccacctt tctcaaggat
     2281 ctgaatgtat aactcgcgca ccagcggctc ggcggcggtt gtcccttcga tcaggactcg
     2341 gtcaccaggg acaatacgcg cggagtgctc aactaaaact ttcgcgaact tttgaacgcg
     2401 tgaatcagac aaaggaattc tcctgaactg gaattaaagc gcacaaatta taacatttca
     2461 cttgcggtat actccttact cgtggagatc agagagtaga gcctagaaat cagttggagg
     2521 tcctgttgac tgtacaggga ttgggacggc tgaaagtttg ggttagggcc aaagactttg
     2581 ccttgaaaat ttacgaacag gtattaccgc aattacctgc tgaggaaaag tggaacctga
     2641 accagcaaat taggcgttcc tccttgagta tctccgcaaa tattgcagaa ggatatggca
     2701 ggttttattg tcaagacaat gttcgatttt gttatttggc acgcggttca ttggaagaaa
     2761 cactgagtca cctggttttt gctgttgagg cgaaatatat tcctgtcaca ctttatgaag
     2821 ccttagcctc tgacggggaa gaaatcgata aaatgctaaa tggctatatc gcctttctca
     2881 agaaaagcaa acaaggcgca aaagaacctg gtgggaatta ttcccttcag gaagaactta
     2941 cacctttttc ccccgagcct tttgaaagcc ccacagatat ccaagaaatt gatctctaaa
     3001 ccctaataat ctgatctctt cctctatgaa acagctcctc caaaacatca aaaacggaaa
     3061 atccatcgtt gaggacgttc ccattccgac ccctcgggag gggcaggcac ttgtcaaaac
     3121 ggaggcaagc ctggtctccg ccggaaccga gcgcatggtc gttgagttcg cagagaaaag
     3181 tctggttggc aaagcgcgct cacgtccaga cctggtcaaa caggtcctcg ataaagcccg
     3241 gcgtgaaggc gtgctcaata cagcccaagc agcgttcaac cggctcgacc agccaatggc
     3301 attgggatat tcctcagcag gcactattgt tgcgctgggt gaaaagatgg aaggttttcg
     3361 agttggtcag cgggtggcat gttccggtgg tggctatgca gtccatgcag aatatgctgt
     3421 tgtgccgcgt aatctattaa ctcccttgcc tgaaaatgtc gattttgagt ccggggcgtt
     3481 cacaacctta ggtgccatcg ctttgcatgg attccgcttg gcagaaccgc agatcggcga
     3541 ggctgtggcg gtcattggac tggggttatt gggattattg acagcgcaaa tcgcttccgc
     3601 tgccggatgt aatgtattag gtatcgatat cgacgccgaa cgcattgacc ttgcctcctc
     3661 ccttggattg gatgccgttc cgcgcgaaaa agcgattgac tcttctgccg cgttcactaa
     3721 taaccgcggc ttcgatgtaa ttctgatctg cgcggatact gcctctgatg accctgttga
     3781 attggcagga gctatcgccc gtgaccgcgc tcgaatcgtt gcaaccggcg cggtgggatt
     3841 gaacatcccg cgtaaagtct attacgaaaa agagatttca tttatcaact ctcgttcgta
     3901 tggtcccggt cgttatgatt cttcgtatga agaagatggc aaggattatc cgctcggcta
     3961 cgtccgctgg acggaaggtc gtaacttcga agctgtcata gatttgatag cgaaa
//
LOCUS       DASTJP010000651         7385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1215916, whole genome shotgun sequence.
ACCESSION   DASTJP010000651 DASTJP010000000
VERSION     DASTJP010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215916"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFQ23_19095"
     CDS             complement(<1..208)
                     /locus_tag="VFQ23_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908764.1"
                     /translation="MKTNWFRFSLVLLLMMALNVQLVQADTVVHTYVPIGSDYAADTL
                     ESFARSASQRDTNGVVDILVLPITY"
     gene            451..1293
                     /locus_tag="VFQ23_19100"
     CDS             451..1293
                     /locus_tag="VFQ23_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908765.1"
                     /translation="MSSLRTTVARLWTRDSSILLGGFLVTVFLIVYIWWPLAEEYLAY
                     VDWNGEWWRYMDWLLLGIFAFMSLTIIARADLKTDLLIIFVGICGGLGIESWGTQTNL
                     WFYYTDERPPLWIIPAWPIASLSIDRITRSLIFLTTKVTKKHQEKSFVHVVSFVFKIL
                     YWLTFASFLVLMLDFIAPTFDKSFTWFSLILCVLLILTPTDHRFAFLTFIAGSGLGYY
                     LEVWGTTRQCWTYYTYETPPLFAILAHGMAAVVFWRAGLMLKMLWGRFGLSRLHQPSR
                     IEEA"
     gene            1466..2128
                     /locus_tag="VFQ23_19105"
     CDS             1466..2128
                     /locus_tag="VFQ23_19105"
                     /inference="COORDINATES: protein motif:HMM:NF044435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9908766.1"
                     /translation="MPKPTFFNLPEEKRETIFKAAVEEFAEYGLENASTNRIVKNSGI
                     AKGSFYQYFEDKQDVFMHMLAVIERMEMEYFKDRHPPSTSLDTFQYFRWMLKTGMEFG
                     LAHPRMIQAAWRVLLGEGLYYGTRLGDYRQKTTRALSTMIKDAIERGEVDPSIDVEMA
                     VMVMETWSNAITNYVLNEGMKQKDVLKWMRSKKTQETIDKLLYVMEYGLRKTESEFIR
                     KP"
     gene            2223..3452
                     /locus_tag="VFQ23_19110"
     CDS             2223..3452
                     /locus_tag="VFQ23_19110"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9908767.1"
                     /translation="MILYLRLAWRNIWRHRRRTVIIVVALGLSLAMMMMYDGLVDGFN
                     NAIYGNAIRVLGGNIQVRAEGYSEKVDSNPLIPLQNDETIIQTALAHPDVTAAFRRIQ
                     TGGLLSNREGAFPMSIIGIEPEAEAPVSLVAENVVDGRWLTASDEDSILIGRGLADVM
                     GLKVGDRVTMVGSDAHKQNRQRTMTVVGIYELGMPSIEKGTVYISLAEAQSLFDLRGQ
                     STEVQVNLKDLGQENAVVDVLAPALPGYEVASWADNYPELSTAITSKNFVMDIFSVII
                     ELIVGIGVLNLLLMAVFERTREIGLLGAMGLKPRQIASLFILEGTMIGIVGVVAGIAF
                     GVLFNGSFGQIGFDYSSFAGVADYMALISGRVYPSLGLGKLFERALTVLIITTLAAWI
                     PAREASRREPAQALHYV"
     gene            3468..4190
                     /locus_tag="VFQ23_19115"
     CDS             3468..4190
                     /locus_tag="VFQ23_19115"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET9908768.1"
                     /translation="MESNRVLYTHIEKTNLLLICGAIAGPFFTLAWILQGLSRSNYDS
                     RQHAISSLSVGEFGWMQISTFIITGLLILAFSIELRRSLQKSSFWGPLLIGLLGVGLV
                     GSGIFVTDPLNGYPPGTPLIPTDRTTQGILHDLFGIPVFLGLPIACLVFARWFARHGA
                     PHWASYSRLSGIGMFVVFFVARLGFRLLPGYPTLAANFGLLQRITVTIGFAWLTLLAI
                     YMMKTPAEPPSQGRNSPEGKGI"
     gene            4193..5410
                     /locus_tag="VFQ23_19120"
     CDS             4193..5410
                     /locus_tag="VFQ23_19120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908769.1"
                     /translation="MAQLFKMAFRDLGRNKRRSILSALAVTIGTTLLLFMASVIRGEF
                     RGALQNGIRLQTGHLQVRAASYDENKISLAWEDMVDNPAQITDQLNALPQVLDATPRL
                     IASGILSLADHSRGVQVIGIDPDSEANQIFRQGVIAGEFITGDDREGILIGRPLADRF
                     DLNVGDRASLLINTSNGDVDEQLFTIRGIYTTNVFPYDESTVFMPLAKAQTFSGADNH
                     ASLIFVLLKDQEQTEPVAASLTSSNYQVVTWVDLNELSVQFEQFAGAYMIILYLIILG
                     ITATVVTNTLVMAVYERTREIGILSAIGMKGRRIMSAFLTEAALLATGGVIGGLILGG
                     LIAAYVGRVGLVLPATQGVTGILLGDKIYPYLTLQDVISLTIVAYVVTLLASLYPATL
                     AARMEPVEALHAQ"
     gene            5427..6122
                     /locus_tag="VFQ23_19125"
     CDS             5427..6122
                     /locus_tag="VFQ23_19125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908770.1"
                     /translation="MEVTKVTNATRVFKIGEVETRALRGVDLSIQNGEFTALVGPSGS
                     GKTTLLQLIGLLDQPTTGNVAINGQDATHLNRNQRADLRKNAIGFVFQFFALIPTLTA
                     YENVEMPLLLNGTKPAERKKRVNEMLEAVGLSDRAHHRPDQLSGGQQQRVAVARVLAT
                     NPKLILADEPTANLDTENGKQVMDIMQRLNKETGVTFVFATHDPRVIGYANRVVTLQD
                     GLITKDSQSNGTH"
     gene            6130..7059
                     /locus_tag="VFQ23_19130"
     CDS             6130..7059
                     /locus_tag="VFQ23_19130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9908771.1"
                     /translation="MKNLYTLRAPEKHRDYSERSGARRLSSPMQFGAYRNQAPRRRSA
                     AFTVTIIMIALLLTACGVVSTPTPIPTVVLDNNSSTSNNQTDDANSISASAVVVPIND
                     AQLSFTTVGKVTEVNVKVGDQVQAGDVLVQLDTTILDAKVKEAEANVIAAQAQINYLN
                     RLNTDALHLESAQAELVRAQALLDSAKATLASQSTLTAPFAGTIVSVDISPAETVTPG
                     KMVILLGDLSQYQIETTDLSERDVPRVQVGQPVSIFIEALNQEFTGEVIRVSQLSSTL
                     GGDVVYPVTIELSEQPDGLRWGMSADVAIEIGE"
     gene            7063..>7385
                     /locus_tag="VFQ23_19135"
     CDS             7063..>7385
                     /locus_tag="VFQ23_19135"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET9908772.1"
                     /translation="MKYLRLFVLVALLITSSHSAQTALASPRAQEVVSASAVVVPVQV
                     TELGFVSSALLKEITVREGDLVQAGQTLAALDAPEAQYTVAAAEAALRSAEAEAKVQS
                     YRRVKV"
BASE COUNT         1743 a         1921 c         1886 g         1835 t
ORIGIN      
        1 aataggtaat cgggagtacg aggatgtcca ctacgccatt cgtatcgcgt tgagatgccg
       61 aacgtgcaaa gctttcgagg gtgtctgctg cgtagtcgct gccgatcgga acatacgtgt
      121 gaaccacagt atctgcctga acgagttgaa cgttgagagc catcattaag agcaggacca
      181 aactaaatct aaaccaattc gtcttcatcg gttctccttc atgtataaga ttttctgata
      241 ctttgatctt gcgttctgaa gctacgttgt ctgatttacc aaaccacatg caccagcaac
      301 ctcccacaaa gttcgtggga gatggatgcc ccatagttgt ccgattttag aggaaggctg
      361 gcaaaaagac aaggtgacct gtaacctatt gctcatacat cgtatctttg gcttctgcta
      421 tgctgtttac aggcattgta taattctaca atgtcatcac tccgcaccac cgttgccaga
      481 ctctggaccc gcgatagcag tattctgctg ggcgggtttt tggttaccgt tttcctgatc
      541 gtttacatat ggtggccgct ggcagaggaa tatcttgcct atgtagactg gaacggcgaa
      601 tggtggcgct atatggattg gctgctgctg ggaatctttg ccttcatgtc gctgaccatc
      661 atcgcccgcg cagacctcaa aaccgacttg ttgatcatct tcgtcggcat ctgcggcgga
      721 ctggggattg aatcgtgggg cacccaaaca aacttgtggt tttactacac cgacgaacgt
      781 cccccgttgt ggatcatccc cgcctggccc attgcatcac tctcgattga tcgcattaca
      841 cgcagtctaa ttttcttaac gacgaaggtc acaaagaaac accaggaaaa aagctttgtg
      901 cacgtagtgt cctttgtgtt taagattctt tattggctca cctttgcatc atttcttgtt
      961 ttaatgttgg atttcattgc tccaaccttt gacaaatcct ttacgtggtt ctcgctaatc
     1021 ttatgtgttc tgctcatcct cacccccaca gatcatcgct tcgcattcct cacttttatc
     1081 gccggctcgg ggctgggata ttacctcgaa gtgtggggta ccacgcgtca atgctggacg
     1141 tattacacct atgagactcc gccgctcttt gccatactcg ctcatggaat ggcagctgtc
     1201 gtgttttggc gggcgggatt gatgctgaaa atgctctggg ggagattcgg tttgtcgagg
     1261 ctacatcagc cttcgagaat tgaagaagcc taactctttt atacatccta acagccaaac
     1321 cattccccat ccggggaatc agttggtctc gatacagact tcgtctcctc gaccatcggc
     1381 gttctctctc ataaatctat tgacacgggg gcaaactgtc ctataatgtg acccgcaggt
     1441 catatgactt cgaggtcaca tttgtatgcc caaacccacc tttttcaacc ttcctgaaga
     1501 aaaacgtgaa actattttca aagcagctgt tgaagaattt gctgaatatg ggttggagaa
     1561 cgcctccacc aatcggattg taaaaaatag tggcatcgcc aaaggaagtt tctaccagta
     1621 ttttgaagac aagcaggatg tgttcatgca catgctggct gtgatcgaac gtatggaaat
     1681 ggaatatttc aaagacaggc atccaccaag taccagcctg gataccttcc agtacttccg
     1741 ttggatgttg aagactggca tggaatttgg gctcgcccac ccgcgtatga tccaggcggc
     1801 ctggcgtgta ttgcttggcg aaggactcta ttacggtacg cgtcttggag actatcgcca
     1861 gaaaacaacc agggcgctca gcacgatgat caaagatgcc atcgaacgcg gcgaagtgga
     1921 tcccagtatc gatgtcgaaa tggcagtcat ggtgatggaa acctggagca acgccattac
     1981 caattatgtg ctgaacgaag gcatgaagca aaaggatgta ttgaaatgga tgcgctccaa
     2041 aaagactcag gaaacaattg acaaactctt gtatgtcatg gaatacggtt tgcggaaaac
     2101 agaatcagaa tttatacgaa aaccctagag atgtagcgaa attagtcgtc agttagttca
     2161 tcgggaactc gtttcctcaa tcaaagaaac ccttagggtc tgaaattcga aaggaactaa
     2221 aaatgatctt atatctccgc cttgcctggc gcaacatctg gcggcaccgc cgccgcacgg
     2281 tcattatcgt agttgcgctt ggtctttccc tcgccatgat gatgatgtac gacggcctgg
     2341 tagatggctt taataatgca atttatggca acgccattcg ggtcctgggc gggaatatcc
     2401 aggtccgtgc cgagggctac agtgaaaaag tggacagcaa tccactgatc ccgctgcaga
     2461 acgacgaaac gataattcaa accgctctgg cacaccccga tgtaactgcc gctttccgac
     2521 gcattcaaac tggtggactg cttagtaatc gcgaaggcgc ctttccgatg agcatcatcg
     2581 gcattgaacc agaagccgaa gcaccggtca gcctggtcgc tgaaaatgtt gtggatgggc
     2641 gctggttgac tgccagcgat gaagattcga ttcttattgg ccgtggactg gcggatgtga
     2701 tgggcttgaa ggttggggac cgcgtcacga tggttggaag cgacgcgcat aaacagaatc
     2761 gccagcgcac catgaccgta gttggcattt atgaactggg catgccatcc atagagaaag
     2821 ggactgtcta tatttcgctg gcagaagcac aatccctgtt tgacctgcgc ggtcagtcaa
     2881 ccgaagtaca ggttaatttg aaggacctgg ggcaggaaaa cgctgtggtg gatgtattgg
     2941 ctcccgcact gcctggctat gaagtggcct catgggcaga caactaccct gaactttcca
     3001 ccgcgataac ttccaagaac ttcgtgatgg atatcttcag tgtcatcatc gagttgatcg
     3061 tcggtattgg cgtgctcaac ctgttgctga tggcggtttt tgaacgcacc cgcgagatcg
     3121 ggcttttagg ggcaatggga ctgaaaccgc gccaaattgc ctctctattc attctggaag
     3181 gcacaatgat cggcattgtc ggcgtggtcg cagggattgc atttggcgtc ctgttcaacg
     3241 gcagcttcgg acagatcggt ttcgattaca gctcgtttgc cggtgtagcc gactatatgg
     3301 cattgatcag cgggcgcgtc tacccttcgc ttggcttggg caaactgttc gagcgcgcgc
     3361 tcacagtttt gatcatcacc acactggctg cctggattcc cgcgcgtgaa gcctctcgcc
     3421 gcgagcctgc acaggcattg cattacgttt agggcagcga gcataagatg gaatcaaatc
     3481 gggtgttgta tacccatatt gagaagacaa atcttctatt aatttgtggt gcgatcgctg
     3541 gacccttctt cacgcttgcc tggatccttc agggtctcag ccgttcaaac tatgattcca
     3601 ggcaacatgc catcagttcg ctatcggttg gcgagtttgg atggatgcag atctccacat
     3661 tcatcattac aggtttgctt atccttgctt tctccattga attgaggcga agtcttcaga
     3721 aatcatcctt ctggggaccg ctgctcattg ggctgctggg tgtgggactg gtcggctcgg
     3781 gcatctttgt caccgatccg ctgaatggat atcctcctgg aactccgttg atccccaccg
     3841 accgcaccac acagggaatc ctccacgatc tctttggcat cccggtcttt ttgggattgc
     3901 caattgcctg tcttgtcttc gcgcgatggt tcgcaaggca cggtgcgccc cattgggcct
     3961 cgtactctcg cctgagcggc atagggatgt ttgttgtatt ctttgtcgcc cggctgggct
     4021 tccgcctgct tccaggctac cccactctgg cggcgaactt tgggcttctg caacgcatta
     4081 ctgtcaccat tggctttgcc tggctgacct tgcttgcgat ttatatgatg aaaactccgg
     4141 cagaaccccc atcacaggga agaaattctc ctgaaggaaa aggtatctga ttatggcaca
     4201 gctcttcaaa atggctttcc gcgatcttgg tcgcaacaaa cggcgctcga tcctatctgc
     4261 tcttgcagtg acgatcggca cgactctatt actcttcatg gcatccgtta tacgcggtga
     4321 gtttcgaggc gccttacaaa atggaattcg tttacagacc gggcatctcc aggttcgcgc
     4381 tgcttcttac gatgaaaata agatcagcct ggcttgggaa gatatggttg ataaccctgc
     4441 gcaaatcacc gaccagttga atgcgctccc acaggttctg gacgctaccc ctcggctgat
     4501 cgccagcggc attctcagcc tggcagatca ttcgagaggt gtgcaggtca ttggcatcga
     4561 cccggattca gaggcaaacc agatctttcg tcagggtgtg attgctggag aattcatcac
     4621 cggcgacgat cgtgaaggca tcttgatcgg acgacccctg gctgatcgat ttgacttgaa
     4681 cgtgggcgac cgtgccagcc tgctgatcaa tacctccaac ggcgatgtgg acgagcagtt
     4741 gtttacgatc cgtgggattt atacaactaa tgtgttccca tacgatgaaa gcacggtgtt
     4801 catgccgctg gccaaggcgc aaaccttctc aggtgccgat aatcatgcca gcttgatctt
     4861 tgttttgcta aaagatcagg agcagactga gccggtcgcg gcttccctga cgtcttcaaa
     4921 ctatcaagtt gtcacctggg tcgacctgaa cgagctcagc gtgcaattcg agcaatttgc
     4981 cggcgcatac atgatcattt tgtacctaat catattgggc atcaccgcca cggtcgtcac
     5041 caatacgctg gtgatggcgg tctacgaacg cacccgcgaa atcggtattc tctcagcaat
     5101 tgggatgaag ggccgccgca tcatgtctgc cttcctcacc gaagcggcat tgctggccac
     5161 aggcggtgtg atcggtggat tgatcctggg tggattgatc gccgcgtatg tcggtagagt
     5221 cggcttggtt ttaccggcta cccagggagt tacgggcatc ctgctgggag ataagattta
     5281 tccttatctc acattacagg atgtaatcag cctgactatc gttgcttatg ttgttaccct
     5341 tcttgcttcg ttgtatcctg ccacgcttgc ggcgcgcatg gaacccgtcg aagccttaca
     5401 tgcgcaataa tcaataggag ttcaaaatgg aagttactaa agtcaccaac gccacgcgcg
     5461 tcttcaagat cggcgaagtt gaaacccgag ccctgcgcgg cgtggaccta tcgattcaaa
     5521 acggtgagtt caccgccctg gtcggaccgt ccggctcggg caagacgact ctgctccaac
     5581 tgattggcct gctggatcaa cctacgaccg gcaatgtggc catcaacggg caggatgcaa
     5641 ctcacctcaa ccgcaaccaa cgcgctgacc tgcgcaagaa tgcgatcggg tttgtattcc
     5701 aattctttgc gctcattcca acgctcacgg catacgagaa cgttgagatg ccgctgttac
     5761 tgaatggaac caagcccgcc gaacggaaaa aacgcgtgaa cgaaatgctg gaagccgtcg
     5821 gactctcaga ccgcgcccat catcgccccg atcaactctc cggtggacag cagcagcgcg
     5881 tggcggtcgc ccgggtgctg gcaaccaatc ccaaactgat cctcgccgat gagccgaccg
     5941 cgaatttaga taccgagaat ggcaaacaag tgatggatat tatgcagagg ctaaataaag
     6001 agaccggtgt gacctttgta tttgccacgc atgacccgcg tgtcatcggc tatgccaatc
     6061 gcgtggtcac cctacaggat ggcttgatca ccaaagacag ccaatccaat ggaacacatt
     6121 agagccagaa tgaaaaattt atatacgctt cgcgctcccg aaaagcatcg ggactacagt
     6181 gagcggagtg gcgcccgtcg gttgagtagc cccatgcagt tcggggcgta tcgaaaccag
     6241 gcgccacgta gacgaagcgc agccttcact gtaacaatta tcatgatcgc gcttctctta
     6301 accgcctgcg gagttgtatc cacgccaaca cccatcccaa cggttgtgct ggataacaac
     6361 agctccactt cgaacaatca aaccgatgat gcgaattcca tctctgcttc cgctgttgtg
     6421 gtcccgatta acgatgcgca gctctcattt acaacggtcg gcaaagtgac tgaagtcaat
     6481 gtaaaagtcg gagatcaggt tcaggctggc gacgttctcg tgcaactgga taccaccatt
     6541 ctggatgcaa aagtgaaaga agcagaagca aatgtaatcg ccgcgcaggc acagatcaac
     6601 tacttaaatc gcctcaacac cgatgctctt caccttgaat ctgcgcaggc agagctcgtc
     6661 cgtgcgcagg cgttactcga ttcagcaaaa gcgacccttg cctcccagtc cacgctgacc
     6721 gcgcctttcg ccggaacgat cgtttctgtc gacatctcgc ccgccgaaac tgtcacaccc
     6781 ggtaaaatgg ttatcctgtt gggagatctt tcccaatacc aaatcgaaac gactgatctg
     6841 agtgaacgcg acgttccccg tgtgcaggtt gggcaacccg tcagtatatt tattgaagcc
     6901 ttgaaccagg aatttacagg tgaagtgatc agggtcagcc aactctcttc gacgctgggc
     6961 ggcgatgtgg tctatcccgt cacaattgaa ctgagcgaac agcccgatgg attacgctgg
     7021 ggcatgagcg cggatgttgc aattgagatt ggtgaataat tcatgaagta cttacgtctc
     7081 tttgttttgg tcgcgttatt gatcacttca agccattcag cgcaaaccgc attggcttcc
     7141 cccagggcac aggaagttgt gagcgcctcc gcggttgtag ttcctgtgca ggtaacagaa
     7201 ctgggatttg tcagttctgc gcttctaaaa gaaatcactg tcagggaagg tgatctggtt
     7261 caggcggggc aaacactcgc agcattggat gccccggaag cgcaatatac agtcgccgcc
     7321 gcggaagctg cgctccgttc cgcggaagca gaagccaaag tccaaagcta tcggcgcgtg
     7381 aaagt
//
LOCUS       DASTJP010000652         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1005344, whole genome shotgun sequence.
ACCESSION   DASTJP010000652 DASTJP010000000
VERSION     DASTJP010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005344"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1855)
                     /locus_tag="VFQ23_19140"
     CDS             complement(<1..1855)
                     /locus_tag="VFQ23_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET9908773.1"
                     /translation="MATTASKKSKPTKKVVTKRVVIKKKVTKKPNLAFKPTKLKLRRP
                     VPSDIEIAQEAKIKPILQVAQELGIRENELELYGPYKAKVKLEILERLKNKPNGKYID
                     VTAITPTPLGEGKTTTTVGLSQALGAHLGKKVITAIRQPSQGPTFGIKGGAAGGGYSQ
                     IIPMEEFNLHLTGDIHAITAAHNLTAAALDARVMHEQQQDDEKLFNALCPPDKKGNRR
                     FSATMLRRLKKLGINKTNPNDLTPEERSRFARLDIDPATITWRRVVDINDRFLREIQV
                     GLGKDEAGFEHRSGYDITVASEIMAILALTTGVEDMRERFGAMVVATNKRGEAVTAED
                     LGVAGAITVLMKDAIKPNLMQTLEGNAAFVHAGPFANIAHGQSSIIADKIALKLADYV
                     VTESGFGADIGMEKFFNIKCRYSGLVPHVVVMVATVRALKMHGGGPKVVAGKPLAPEY
                     TEENLELLQKGVSNLERHIQNARKFGINVIVAINSFATDTDAEVELIRKSALEFGAAD
                     AIKCTHWADGGAGTKKLAEAVVKAAEMKSDFKFLYPLELSIKEKIETICKEIYRADGV
                     DYSPEAEAKIELYTKLGFDKLPMCMAKTHLSFSTDASLKGAPTGFRVLIRDI"
     gene            complement(1948..>2715)
                     /locus_tag="VFQ23_19145"
     CDS             complement(1948..>2715)
                     /locus_tag="VFQ23_19145"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HET9908774.1"
                     /translation="LDVMDDIALQIVTTIQSQPNIRLVLGHGAGSFGHVAASEYKTRD
                     GYPRPSPLIHRERDENEDNYWKGFAEVWYQASSLNRFMMKALHKAGVRAIALPPSSSV
                     IASEGKVSVWETTPIRMALSARIVPVIFGDVVFDEIRGGTILSTEDLFMHLARALSPE
                     RILLAGLEAAVWEDFPARTKKIELITPESFADISKGIGKSTGADVTGGMESKVTQMLE
                     LVENNHDLSIQIFSGREPGYLTRALTGENLGTVIAAS"
BASE COUNT          592 a          698 c          687 g          738 t
ORIGIN      
        1 gtatatcgcg gatcaacaca cggaagccgg tcggcgcgcc tttgagagaa gcatcggtac
       61 tgaaggaaag atgtgtcttt gccatgcaca tcggaagttt gtcaaagccg agcttggtat
      121 aaagttcgat tttggcttca gcttctgggg aataatctac gccgtctgcg cgataaattt
      181 ccttgcaaat cgtctcgatt ttttccttga tggaaagttc cagcggatac aaaaacttaa
      241 agtcactctt catttccgct gccttgacca ccgcttctgc caacttcttc gtacccgcgc
      301 cgccatcagc ccagtgtgta cacttgatgg catccgccgc gccgaattcg agggcagatt
      361 ttcggatcag ttccacttct gcatctgtat cggttgcaaa cgagttgatc gccacaatta
      421 cattgatacc gaacttgcgc gcgttctgga tgtgacgttc aaggtttgaa actccctttt
      481 gaagcagctc gagattctct tcggtatatt ccggcgccaa aggtttccca gctacaacct
      541 tcggacctcc gccatgcatt ttgagcgcac gcaccgtagc aaccatcaca acgacatgtg
      601 gaacgagccc actatagcgg catttgatgt tgaagaattt ttccatgccg atatcagcgc
      661 cgaagccaga ctcggtcacc acgtaatctg caagttttag cgcgatcttg tcagcgataa
      721 tagaggattg tccgtgcgcg atgttggcaa acggacctgc atgtacgaag gcagcatttc
      781 cttccagcgt ttgcatcagg ttaggtttga tagcatcttt catcaatacc gtgatcgcac
      841 ccgccacgcc cagatcctcc gcagtgacgg cttcgccgcg tttgttcgtg gcaaccacca
      901 tcgcaccgaa gcgttcgcgc atatcttcca caccggttgt caaggcgagg atcgccatga
      961 tctcagacgc tactgtaatg tcgtagccgg aacgatgttc gaatcctgct tcgtctttgc
     1021 cgagccctac ttgaatttcg cgcaggaagc gatcattgat gtcaacgacg cgtcgccatg
     1081 taatcgtagc agggtcaata tccagccttg cgaaacggga tcgttcttca ggagttagat
     1141 cgttgggatt tgttttgtta atacccaact ttttcaagcg gcgaagcatt gtggccgaga
     1201 atctgcggtt tcccttttta tccggcggac acaacgcgtt gaagagtttc tcgtcatcct
     1261 gttgttgttc gtgcatcacg cgcgcatcca gggctgcggc agtgagattg tgcgcggcgg
     1321 tgatggcgtg gatgtcaccg gtgaggtgca ggttaaattc ttccatgggg atgatttgtg
     1381 aatatccacc acctgccgca ccgcctttaa tgccaaaggt cggcccttgc gagggctggc
     1441 ggatggccgt aataaccttt ttaccgagat gcgcgccgag cgcctgagat aaacctaccg
     1501 tagtcgtagt tttgccttca ccaagaggcg tgggggtgat agctgtgacg tcgatgtatt
     1561 tcccgttcgg tttgtttttt agtcgttcga ggatttcgag tttaactttt gccttgtacg
     1621 gaccataaag ttcgagttcg ttctcacgga tgcccagttc ctgtgccacc tgcaagatgg
     1681 gtttgatctt cgcctcttgt gcgatctcaa tatcagaggg gacaggtcgg cgaagtttta
     1741 gtttggtggg cttgaatgca aggttgggct ttttggtaac tttctttttt atgacaactc
     1801 ttttcgtaac gaccttcttt gtgggttttg atttcttcga agctgtggtt gccattcttt
     1861 ctcctttgtg agatgttttc tatgatcaaa acaagaacta tatttgtaat aagcggcgct
     1921 gtttgaattg ctctttcgag ggacttatca ggatgcagca atcaccgttc ctaaattttc
     1981 tcccgtcagt gcacgtgtga ggtaaccggg ttctctcccg gaaaatattt gaatgctgag
     2041 atcgtgattg ttctcaacca attccaacat ttgcgtcacc ttggattcca tcccgccggt
     2101 aacgtctgca ccggttgatt tgccaattcc tttactgata tcagcaaacg attccggggt
     2161 gatcagttcg atctttttcg tccgagctgg gaaatcctcc cagaccgcgg cttcaagccc
     2221 tgcgagcagg atccgttcgg ggctaagggc gcgcgcgaga tgcatgaaca ggtcttcggt
     2281 cgaaaggatc gtgccgccgc ggatttcatc aaagacaaca tccccaaaaa taacaggcac
     2341 aatacgcgct gacaaagcca tccggattgg tgtggtttcc caaacggaga cttttccctc
     2401 actcgcaatc acactggacg aaggtggaag cgcgatggcg cgtacacctg ccttatgcaa
     2461 agccttcatc atgaagcgat tcagggaaga tgcctgatac cagacctccg cgaaaccctt
     2521 ccaataatta tcttcatttt catcgcgctc gcgatgaatt aggggcgatg ggcgcgggta
     2581 accgtcgcgc gttttgtatt cactcgcggc aacatggcca aaggacccgg cgccgtgccc
     2641 gagcacgaga cggatgttcg gctgagactg aatggttgtc acgatctgca acgcgatgtc
     2701 gtccatcaca tccaa
//
LOCUS       DASTJP010000653         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_242315, whole genome shotgun sequence.
ACCESSION   DASTJP010000653 DASTJP010000000
VERSION     DASTJP010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242315"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VFQ23_19150"
     CDS             complement(<1..978)
                     /locus_tag="VFQ23_19150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9908775.1"
                     /translation="MDDFHQSVERIDALMGCGNLLSFLELFSHRFSSTTDSPFSLLLV
                     DLNNFMKFNKEHGQEQGDAVLHWVSIVLRDTALPVYRIGGDEVLVVLTGGTPEEKENL
                     ARAVFSRLNRESSQFAWSDPASVMVIHFENEKLEIGDLWIAISDALQAAKVEGGRGFH
                     VSSYTRGISENSYQLGVINMLTERLLSFANRLEVSNQLAYLDPNTQLPNSLSAEREIE
                     HTMQQAQQSNGTFTILFIDGDNLRLYNDISYSAGDEMIRNLASTLSHHLRPGDFLARW
                     RVGDEFFVILPDTPSEKALHVAERLRAAVESESQKWNIPVSISIGMADYP"
     gene            complement(1069..1575)
                     /locus_tag="VFQ23_19155"
     CDS             complement(1069..1575)
                     /locus_tag="VFQ23_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908776.1"
                     /translation="MIELRFKEFYEQNYEDEEYCLYVMKNGLGDVLYVGISTDDVWSR
                     WFGGTGHIPWSGTKRYGNSTIGEKITNHLPESLEWNIQLWTLKDCLAFCEMEPPDPNF
                     KPTAGEYNDAVRTVEARMIKKLSPSLNRHLNLNPGKDTTPKSEKELKWEKYVAAAYDE
                     IFNKKTKG"
     gene            complement(1572..2594)
                     /locus_tag="VFQ23_19160"
     CDS             complement(1572..2594)
                     /locus_tag="VFQ23_19160"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9908777.1"
                     /translation="MPDNSLTISDVINNYLRMVERARSKHTMLAYKNALQVFSRILEK
                     KSLKPDVTPIGKLTEESFAKFIDHLSVYANATEQMYLQAVKGFYLYVDSEKLIDVNQS
                     RLVVLMRQRARRPGTRIPQSEINDIEAILEKVQDVENLRFSVVGKDEEPNVKLRAYRD
                     RAFLLTLADTGFRVHEACNLRRGDVDWNEGRAIIIGKGNKQAVVRFTSRSMQAIKDYL
                     SQRAVLDGSSGKQLSSLPLFARHDKGAGKKVKPMTTTTGRNLVRERVEQLLGKEMVGK
                     ITPHSFRHYFVTSALRGTGNLAIAQKLARHQNIQVTQSYTHLSDEELDKAYYEVFENN
                     SKGSKE"
BASE COUNT          683 a          630 c          600 g          750 t
ORIGIN      
        1 cgggtagtct gccataccaa tggatatgga aaccgggatg ttccattttt gcgactcact
       61 ttctacggcc gctcgcaggc gctctgccac atgcagcgct ttctcgctgg gagtatccgg
      121 aagaataacg aaaaattcgt cgcccacgcg ccaacgggcg aggaagtcgc cagggcggag
      181 gtgatgagac agcgtgctgg caagattgcg gatcatttca tcgccggctg aatagctgat
      241 gtcgttatat agtcgcaagt tgtcaccgtc aatgaataag atggtaaacg ttccgttgct
      301 ctgttgtgcc tgctgcatgg tgtgttcgat ctctcgttcg gcagaaagac tattggggag
      361 ctgggtattg ggatccagat aggccaactg attggacact tccaatcgat tggcgaatga
      421 aagcaaacgt tccgtgagca tattgatgac ccccaattga taactattct cagagatgcc
      481 acgcgtataa ctgctgacgt gaaaaccacg accaccttcg acctttgcag cctgaagggc
      541 atcgctgatg gcgatccaca gatctccgat ctccagcttt tcattctcaa aatgaatgac
      601 catcaccgac gccggatcgg accaggcaaa ctgagaactt tcgcgattca gacggctgaa
      661 gacggcgcga gccaggtttt ccttttcctc aggcgttcct ccggtaagca caaccagaac
      721 ttcatcacca ccaatgcgat agaccggaag cgccgtatcc ctcaaaacaa tgctgaccca
      781 gtgcaggacg gcatctccct gttcctgccc atgttccttg ttgaacttca taaagttatt
      841 gaggtcaacc agcagcaggg aaaaggggga atctgttgta gaactgaacc gatgagaaaa
      901 caattcaagg aaggaaagca gatttccaca ccccattaat gcatcaatgc gttcgacgga
      961 ttgatgaaaa tcgtccattg ttgctctatt tttatcataa attattgttt cgatatagat
     1021 tgattgtcag acgtacaatt ggatttttac tagccgtaat gcgctcaatc aacccttggt
     1081 tttcttgtta aaaatctcat cgtaggcagc ggccacatac ttctcccatt tcaactcctt
     1141 ttcacttttt ggtgtggtgt cttttccagg attcaaatta agatgcctat ttaacgaagg
     1201 agataacttt ttgatcatgc gagcctccac agtgcgaact gcatcattgt attcgccagc
     1261 agtcggcttg aaatttggat caggaggttc catttcacaa aaagcgaggc aatctttcag
     1321 tgtccatagt tggatattcc actccaatga ttcaggcagg tgattcgtga tcttttcacc
     1381 aatcgtcgag ttaccataac gcttcgttcc tgaccaggga atgtgtcccg ttccgccaaa
     1441 ccaacgagac cagacatcgt cagtggaaat tccaacataa aggacatccc ccaatccgtt
     1501 tttcattacg tacagacagt actcctcgtc ttcataattc tgttcataga attccttgaa
     1561 tcttagctcg atcattcctt actccccttc gaattatttt cgaaaacctc atagtaggcc
     1621 ttgtcaagct cctcatcgga tagatgagta tagctttgtg tgacttgaat attttgatga
     1681 cgcgcgagtt tctgggctat tgcaaggttg ccggttccgc gtaatgccga agtgacaaaa
     1741 taatgacgga aggaatgggg ggtgattttc ccgaccatct ctttgcctaa caattgttcc
     1801 acacgctctc tgacaagatt gcgtcccgta gtggttgtca tcggtttgac cttttttcct
     1861 gcgcctttat catggcgggc aaagaggggg agagaactta attgtttgcc agaacttcca
     1921 tccaacaccg cgcgctgtga caaataatcc ttgatggctt gcattgaacg agacgtaaaa
     1981 cgaaccactg cttgtttatt gcctttaccg ataataatgg cacgtccttc gttccaatca
     2041 acatcgccgc gtcggagatt acaggcttca tgaacgcgaa agcctgtatc agccaatgta
     2101 agcaggaatg cccgatctcg gtatgcgcgt agtttaacgt ttggttcttc atccttgccg
     2161 acaactgaaa acctcagatt ttcgacatct tgaaccttct ctaaaatggc ttcgatatca
     2221 tttatttcgg actgtggaat gcgggtacct gggcgccgtg cgcgttgtcg cataagtacc
     2281 accaggcgag actgattaac gtcaatcaat ttttccgagt caacatacag gtagaaacct
     2341 tttacggcct gtagatacat ttgctctgta gcattggcgt agacgctgag gtgatctata
     2401 aatttagcga atgactcttc tgtgagtttt ccaatggggg taacgtcggg cttgagtgat
     2461 tttttttcca ggattctact gaaaacttgt agagcgttct tatacgcaag cattgtgtgt
     2521 ttactacggg cgcgttccac catacgtaga tagttattta ttacatcaga aatcgtcagt
     2581 gaattatctg gcataggttc attatacgaa tttcccaagg attgtcaatg ctttcgataa
     2641 taatacttat cgaaagcata aag
//
LOCUS       DASTJP010000654         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_868124, whole genome shotgun sequence.
ACCESSION   DASTJP010000654 DASTJP010000000
VERSION     DASTJP010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868124"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..2327
                     /locus_tag="VFQ23_19165"
     CDS             453..2327
                     /locus_tag="VFQ23_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9908778.1"
                     /translation="MKPTKFFIVVLVTLSLMLGACQPAGTSEPVATKEPVATGRPVAE
                     EPADPNALPRNETLYFNGLQWNPLKGWNPYSSDMNNAMGIGDGGNNYSAQIPVFETLY
                     MFNMLDGKVYPLLADGDYVWNDAMTELTVRIKKAAHWSDGTPVTAEDVAYTYASNLKY
                     ETSNGANYKPYIQSIDVVDPQTVVIKAVLNKEGQAANPLVVAQYLSHVYVNQKAFTQK
                     LEERVAGDPAAFKADPAEDAIGSGPYLPYFADESKVVLIRDDNYWGQADSMWGKLPVP
                     KYLAHVIFKDNAAGFTALQAGEVDVSQQFNANVQDLWLKDGLPVSTYLADAPYMVAAS
                     LPTAYFNLNSYGLDNVAVRQAIAMAVDYDTIIANAMTNQSPTFAQVPRSIMNPTAGEQ
                     AMYDQTAVKNLQWTGKDIESAKALLDAAGIKDTNGDGWRELDGKKLSYKALCPNGWTD
                     WQAAIEIVAAAGEKIGIDITTEFPEWSVYQTVFTDAKVNNYDIFMVWSNGASPTSPWG
                     RARQLMSSEFVGTSNNWNGNWGGYSNERVDELLALIPHETDQAKLKEYYTELTEIYLT
                     DVPSFTLMYRPDQFHTVNESVWTNFPNAEDGTNPPVPPTNLVKGFGIAGLYNLTLVGD
                     "
     gene            2405..3409
                     /locus_tag="VFQ23_19170"
     CDS             2405..3409
                     /locus_tag="VFQ23_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908779.1"
                     /translation="MKGYRKYFLNKLAWFLVTFVIAFGLNFILPRLMPGDPVAAIMNR
                     AAAGMTNQTAIQQVYERYTELFGTNKPMHEQFFLYVKNAFRGDFGISFSQYPRRVSDI
                     IGTSILWTLALQFPAILIGWIIGNTLGALAAYNKGGFDKLLMPVSIFLSNIPPFGMAI
                     ILLVTLAVNAKWFPTSGGYGFDLIPSFTWSFGWSVIVHYQLPFWSIVLIAIGGWAIGM
                     RSMAIYELNADYVKYSRFMGIKDRKILAYVFRNAMLPQVTGLALAIGTMVSGALVAEI
                     IFSYPGLGTTIMTAVLGRDYPLISAATLVITVMVLTANFIIEILYGLIDPRIKTAQAD
                     "
     gene            3424..>3656
                     /locus_tag="VFQ23_19175"
     CDS             3424..>3656
                     /locus_tag="VFQ23_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908780.1"
                     /translation="MKHTLRQIFHSGKFTFGFAIFMLIVLIVILYPLIIRERPLAIIG
                     QGTFFPPGIYVSVYDSIGSPTYTINLDDAAANR"
BASE COUNT          907 a          924 c          925 g          900 t
ORIGIN      
        1 aacggaataa atgagtgtaa acaagagaat caatcataca aagccgttcc caatcatttc
       61 catgccagtt gcgaatgaag gtactgtata gtgcccgtgg ctcatcctca gttggcgctg
      121 cgggcatttc acatgctccc aggcacttat cctttatcag tgtgcccatg gatttagttc
      181 aacccaattc cgtttcaaat cacaaccatc cactcggata gtaataacca gccagttggt
      241 gggggagttt tttcgagaaa tccatatagt cacaatagag caaatagaaa tattcccggt
      301 gaggaatttt caaatgtcta atattcgggc gtgctcggat ctggcatgtc ccctgatcgc
      361 gaggaaagga gaagattatt gatgaaagaa agaaacacta ttttttcacg cctattgaga
      421 actcaacaaa gcaagataag gagaattcag agatgaaacc cacaaaattt tttatcgtgg
      481 ttctggtgac tctaagcctg atgctcggag cctgtcagcc ggcgggcaca tcagagcccg
      541 tcgccaccaa ggaaccggtc gcgacgggac ggcccgttgc ggaagaacct gctgacccga
      601 atgccttgcc ccgcaacgaa accctttact tcaatggtct tcagtggaat ccgcttaagg
      661 gctggaaccc atattcctcc gatatgaaca atgcaatggg cattggggat ggcggcaaca
      721 actacagcgc gcagattccc gtgttcgaaa ccttatacat gttcaacatg ctagacggta
      781 aagtatatcc gctgctggca gatggtgatt atgtctggaa cgatgccatg accgagctga
      841 ccgtcaggat caagaaagct gcccattgga gcgatggcac tccggtgacc gccgaggatg
      901 tggcttatac gtatgcatct aatcttaagt atgaaaccag caacggcgcg aactacaagc
      961 cttacatcca atccatcgac gtagtggatc cacagacggt tgtgatcaaa gcagtattga
     1021 acaaggaagg ccaggcggcg aaccctctgg tcgttgccca atacctcagc catgtctatg
     1081 tgaatcagaa agccttcacc caaaaactgg aagagcgtgt cgctggcgat ccggctgcct
     1141 tcaaagctga tcctgcggaa gacgccattg gctcaggacc ctatctcccg tacttcgcgg
     1201 acgagtccaa ggttgtcctc attcgtgatg ataactactg gggtcaggct gattccatgt
     1261 ggggcaagct gcccgtgcca aagtaccttg cgcatgtgat ctttaaggat aatgccgccg
     1321 gctttaccgc attgcaagcc ggtgaggtgg atgtcagcca gcagttcaat gccaacgttc
     1381 aggacctgtg gttgaaagat ggactgcccg tttctaccta cctggccgat gcaccttaca
     1441 tggttgctgc cagcctccca acagcttact tcaacctgaa ttcgtatggt ttggacaatg
     1501 tcgcggtgcg ccaggcgatt gccatggcgg ttgattatga caccattatt gccaacgcga
     1561 tgaccaacca gtccccgacc tttgcccagg ttccacgctc gatcatgaac ccgaccgctg
     1621 gtgaacaggc gatgtacgat caaactgccg tcaaaaactt gcagtggact ggcaaggata
     1681 ttgaaagcgc taaggcgctt cttgacgcgg ctggtatcaa agatacgaac ggcgatggct
     1741 ggcgtgagct cgatggaaag aaactctctt acaaagcgct ctgcccgaat ggctggaccg
     1801 attggcaggc ggcgatcgag atcgtggctg ccgctggcga aaagatcggc atcgatatca
     1861 cgaccgagtt ccctgaatgg tccgtgtacc agaccgtctt caccgatgca aaagtgaaca
     1921 actacgacat tttcatggtc tggagcaatg gcgcaagccc aaccagtcca tggggccgcg
     1981 ctcgccagtt gatgagctct gagttcgttg gcacatccaa taactggaac ggcaactggg
     2041 gcggctactc caatgagcgc gtcgatgagc tgcttgcctt gatcccgcat gaaacggatc
     2101 aggctaaact aaaagaatac tacaccgagt tgacggagat ctatctgacg gatgttccct
     2161 ccttcactct gatgtaccgc ccggaccagt tccacactgt gaacgagagc gtttggacga
     2221 acttccccaa cgcggaagat ggaaccaatc cacccgtccc gccaaccaat ctggtcaaag
     2281 gtttcggtat tgcaggttta tataacctca cgcttgttgg tgactagacc ataatttagt
     2341 aaaacagcca tgttccggtt tctacggaac atggctgtcg aatacggcac gatgaggtgt
     2401 gacggtgaaa ggctaccgaa agtacttcct caacaaactg gcctggttct tagttacctt
     2461 tgtcattgca ttcgggctga actttatcct gccacgcctg atgcccggcg accctgtggc
     2521 ggctatcatg aatagggcgg cggcaggtat gaccaaccag actgcgatac aacaagtgta
     2581 tgagcgatac acagaactgt tcggcacaaa caaacccatg cacgaacagt ttttcctgta
     2641 tgtaaaaaat gcattccgag gcgattttgg tatttcgttc agtcaatatc cccgtcgggt
     2701 atcggatatt atcggcacct ctatcctatg gacattggct ctacagtttc ctgcgatcct
     2761 aatcggctgg attataggga acacactcgg tgcgctggcg gcctataaca aaggaggctt
     2821 tgataagctt ctcatgcctg taagtatttt tctcagtaac ataccgccat ttggtatggc
     2881 aattattttg ctggtcactt tggcagtcaa tgcgaagtgg ttccctactt ccggtggata
     2941 tgggtttgac ctgattccca gttttacctg gtcatttgga tggtcggtga tcgtccatta
     3001 tcagttgccg ttctggtcga tcgtgttgat cgcgattggc ggctgggcga ttggtatgcg
     3061 ctccatggcg atctatgagc tgaatgcaga ttacgtaaaa tacagccgct tcatggggat
     3121 caaagatcgc aagatcctcg cctatgtgtt tcgaaatgca atgctcccac aggtgaccgg
     3181 tctggcactg gcgatcggca ctatggtcag cggtgcgctg gtggcggaga tcatttttag
     3241 ctacccaggt ttgggaacca cgatcatgac agccgtgttg gggcgggatt atccgctgat
     3301 ctcggcggct acgctggtca ttacagtgat ggtgttaaca gctaacttta ttattgaaat
     3361 tttatatgga ctcatcgacc cgcgcattaa aaccgcgcag gccgactaag cagggctgct
     3421 gcgatgaaac acacgctgcg acaaattttt cactccggca aattcacttt tggttttgcg
     3481 atcttcatgc taatcgtact gatcgtgatc ctttatccgt tgatcataag agagcgtcca
     3541 ctcgcgatca tcggtcaggg cacgtttttt ccgccgggta tctatgtgag tgtttatgac
     3601 agtattggct cacctaccta cacaattaat cttgacgatg ctgccgccaa tcgaat
//
LOCUS       DASTJP010000655        10690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1017642, whole genome shotgun sequence.
ACCESSION   DASTJP010000655 DASTJP010000000
VERSION     DASTJP010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10690
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017642"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFQ23_19180"
     CDS             <1..209
                     /locus_tag="VFQ23_19180"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9908781.1"
                     /translation="VADWYSDNYYRDASLANPVGPISGDYRVIRGGAWYVRDFYTRTT
                     VRGYYGPDGATSGIGFRCAMSAFE"
     gene            369..2225
                     /gene="typA"
                     /locus_tag="VFQ23_19185"
     CDS             369..2225
                     /gene="typA"
                     /locus_tag="VFQ23_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET9908782.1"
                     /translation="MITERTDLRNIAIIAHVDHGKTTLVDGLLRQSHTFRDNQQVAER
                     VMDSNDLERERGITILAKNTAITLVEPATNKPIKINIVDTPGHADFGGEVERVMNMVD
                     GVLLLVDAAEGPMPQTRFVLKKALQMGHKAIVVINKIDRKDAEPERVLNETFDLFIEL
                     GASEDQADFPVIYAQATAGRAGLAKELGPDLQPLFDVILKQIPAPKVDVDAPLQLLVT
                     TLGYDDYRGVTAIGRVFAGKIKAAQRLARLTVDGRNLPESAKYLYTFQGLNKIEIDEA
                     SAGDIISLAGLEGIAIGETLADPNDPIALPTIKVEEPTVRMTFGVNTSPFTGKEGTYS
                     TSRNLRERLFEELRTNVSLRVDETESAENFLVSGRGELHLGILIETMRREGYEFQVSR
                     PEVIFHKADDGALLEPYEEVHVETSSETVGVVVEMLGSRRGQMMDMQDSGQGTTRMVY
                     VVPTRGLLGFRYQFLTSTRGAGVMHTIFHGYDEMAGPMSTRQSGSLVAWEAGTTTAYA
                     LKSAEERGVLFYGPGVDVYAGMVVGENARSGDITVNVCKKKHLTNVRASRGDMEIRLT
                     PPRQMSLDEAIEYLSDDELLEITPEAYRIRKRILNSDDRGKQTKKVKELMEE"
     gene            complement(2259..3035)
                     /locus_tag="VFQ23_19190"
     CDS             complement(2259..3035)
                     /locus_tag="VFQ23_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9908783.1"
                     /translation="MSHNLKSLLAGTVLLLAALACNGPFTNAQQPTDAEIQTAAAETL
                     QAIFTPSDSTPTQQSATEVPTDIAPGATATPTGAVTRTPTYSVPMLTVIEQTNCRSGP
                     GQDYEILFTYLPGKELEILGRYDPDNYWLVKAEESPTGNCWLWGEYVELSGSFSAVSS
                     VTPPPTSTQAPPQAPSIQEWEFSCSGGILTFTVSWIDKSSNETGYRIFRNGEAVAEIP
                     ANSSTYTDSFARLAGENAEYYLQAFSPFGTANSSVMRMTC"
     gene            complement(3199..4761)
                     /locus_tag="VFQ23_19195"
     CDS             complement(3199..4761)
                     /locus_tag="VFQ23_19195"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9908784.1"
                     /translation="MNKRMNRFIVLILTLALALPAQFVLADDISETQGFRKHVTLAGI
                     REHQAALQAISNDNGGNRVSGSAGFDASAQYVYDRMVAAGYNVSFQEFTFNFDGDASA
                     PALEQTSPTPTVYIDGVDYDTMQFSGSGDFTAPLTAVDLLVPSPFANASTSGCEAADF
                     AGFTPGHIALMQRGTCGFVNKAQNAQAAGAVGVIVFNEGNPGRTNLNLGTLGTPQTIP
                     SITTTFAMGQTLRNGVLNGPTGITVRLKVDYFFGPRTTRNVIAETPTGDPNRVIVVGA
                     HLDSVTRGPGINDNGSGSATILEVAEVFASQGRTPRNKLRFMWYGAEELSPGLIGSNF
                     YVANLSQAERDKIELMLNFDMIGSPNFVRFVYDGNNSGGGGAVGPTGSGAIEQMFLEY
                     FASQGLATEPTPFNGRSDYGPFIAPGVDIPAGGLFTGAEGIKTPAQAAVYGGTAGQQY
                     DPCYHLACDTFDNNSNTALDQMSDAVAHSVLLFSKRNFEKNPLVDPVVAGATGSGGGG
                     GLHDHHDESEPN"
     gene            complement(5137..6759)
                     /locus_tag="VFQ23_19200"
     CDS             complement(5137..6759)
                     /locus_tag="VFQ23_19200"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET9908785.1"
                     /translation="MLSKTIRIVALVSIIAAAFSFAPVAMAGPASCGNRNNNTHAKLL
                     ECVTLEGVREHQAAFQAIANDNGGTRAAGTPGYEASVAYVVERLTAAGYNVTLDEFPF
                     LFFPPAVLQQLTPVNAAYESGVFTASGVGAVTASVTPVDINLVPPRANTSGCQGAYTE
                     AAVGAAIVPAAPGSPTDFAGFPAGNIALIQRGGCSFALKAYNAQAAGASAMVLFNQGD
                     TPARSTLIVGTLVVAAGTPGPVTIPVVGGSFAAGESLAQPGSTARVVVEAPRNVTQVN
                     VLAESQGGDPNNVVMAGAHLDSVQAGPGINDNGSGSAALIEVAEQMSKVTPRNKVRFA
                     WWGAEESGLIGSTQYVAGLSQAELDRITLYLNFDMIGSPNHVFFIYDGDNSDGVGSPA
                     GPAGSAEIEKTFEAFFNMRGIPFKGTDFSGRSDYGPFIAAGVDIPSGGLFTGAEGIKT
                     AAEVALWGGTAGQQYDPCYHLACDTFANNNDFALDVNSDAVAFSILQYAMSTQDINDA
                     QGKGNFPPVSGGGSSNTSTSTQSGHDHELPAE"
     gene            7644..8306
                     /locus_tag="VFQ23_19205"
     CDS             7644..8306
                     /locus_tag="VFQ23_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908786.1"
                     /translation="MSESPRPRPRLDPSIIAALIGLVGTMFVAIMTYMANRPASPQAT
                     PIPTVAVVEPGTEMPTLIDTVEVSTQPMAASPATVPTVKYPNGKLFKLFYDDNSFYLL
                     NMSDTAIPINRVAFERLSDADVPLNRFTGTRWAEFYPDSTPGKCVALEIFGSPPYLDP
                     PECGGGVFLSLRTPPRDDPAIFWTAQEGSHQFRVLWREGGEDEEVARCEIGAVTCAVF
                     LP"
     gene            8459..10564
                     /locus_tag="VFQ23_19210"
     CDS             8459..10564
                     /locus_tag="VFQ23_19210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET9908787.1"
                     /translation="MASIKTLEVPIRGMDCVECTQHVGNAISKLEGVKSVDVLLAAEK
                     AIIQLDPEKVDMFSIREAVASAGDAYSVPETVEAVQPLSISDFNSQLTKMLVVVFSIV
                     FSIVILGEWLGVFELLAAFIPFPVGFGIVLLAGWPVFRKVFIATLKRQILSHTLMTLG
                     VIAALAVGEWVTAAIVVIFMRVGEYVENFTTEGARRAVKELIAIAPQTARLERDGAEI
                     EVPLAEVRVGEIVIVRPGERIPVDGEVLSGHATIDQAAITGESMPIEAKNGTYVFAAT
                     IAKLGSLRVKTMRIGADTTFGRIIKLVEEAEANRAVVQRFADKFSAYYLPVVAGIGAL
                     TFLLSRNPLSTAAVLVVACSCSIALATPIAMLASIGASAKRGLLIKGGKYLEALAQAD
                     VILVDKTGTLTLGQPQITDVIPLNGLPPSEILAIAASAERYSEHPLAQAVRDAALKDQ
                     LRLSEPLDFEAIPGQGIRARINGKSVAVGNRRLIPTAASLPIAAQLEEQGKTLLFLEL
                     DNESAGILAAADTLRAEVPAAFAELRRFGIKHIQLLTGDNKRTASALAEKLGVEYRAN
                     LLPEHKIQIVKEYQSKGHTVVMVGDGVNDAPALAQADIGIAMGAAGTDIAIEAAHIAL
                     MREDWSLVPEALYIARRTMGIVKMNLAFTALYNVIGLGLAAFGFLPPVFAAAAQSIPD
                     VGILANSARLLNQKKNQQI"
BASE COUNT         2637 a         2613 c         2799 g         2641 t
ORIGIN      
        1 gggtggcaga ttggtacagt gataattact atcgagatgc ttctctcgca aatccggtgg
       61 gaccaatttc tggggattat cgagtgatac gaggtggcgc atggtatgtc agagattttt
      121 acacccgcac tactgtccgc ggttattacg gtccagatgg tgcaacctct ggcattggtt
      181 tccgctgtgc tatgagtgca tttgaatgaa tcccttccgc aatccataac cctctgatac
      241 aataccgtcc gcacgccgac cgcgaaaccc tcggctccct tgccgggggt ttttgtatgc
      301 aaacttccat cacctctagg ccctctcatt ctaagaaggg ggttgaggtg atggttataa
      361 ggaaataaat gattactgaa cgaactgact tacgcaacat cgccatcatc gctcatgttg
      421 accacggcaa gacgactctt gtcgatggtc ttctacgcca atcccacacg ttccgcgaca
      481 atcaacaagt agcggagcgc gtcatggatt caaacgacct tgagcgcgag cgaggtatca
      541 ctatccttgc caagaacaca gccattactc tggttgaacc ggccacaaat aaaccgatca
      601 agatcaatat cgttgataca cccggtcacg cggatttcgg tggggaagtg gaacgcgtga
      661 tgaacatggt ggacggagtg ttgctccttg tcgacgcagc cgaaggcccc atgcctcaaa
      721 cccgtttcgt gcttaagaaa gcgctgcaaa tgggacacaa ggcgatcgtg gtgatcaata
      781 aaatagaccg taaagatgct gaaccggagc gcgttctcaa tgagaccttc gacttgttca
      841 tcgaactggg cgcgagtgag gatcaggcag atttccctgt gatctatgct caggcgaccg
      901 ccggacgcgc aggtctcgct aaagaactgg gaccggatct tcaacctcta tttgatgtta
      961 ttttgaaaca aatccccgct cccaaagtgg acgtggatgc tcctttacag ttgcttgtta
     1021 ccacattggg ttatgatgac tatcggggtg tcaccgcgat cgggcgtgtc tttgcaggca
     1081 agatcaaagc ggcgcagcgc ctggcgcgtc tcacggtcga tggacgcaac cttcctgaat
     1141 cagcaaaata tctttataca ttccagggtt tgaacaaaat cgagatcgat gaagccagtg
     1201 ctggtgacat tatctccctg gccggactcg aagggatcgc aattggcgaa acacttgctg
     1261 acccgaatga cccgattgca cttccaacga tcaaagtgga ggagccaacc gtccgcatga
     1321 cgtttggagt gaacacctct ccctttactg gcaaggaagg cacgtatagc acatcgcgaa
     1381 atttgcgcga acgcttattt gaagagcttc gcacgaacgt ttcactacgc gtggatgaga
     1441 ccgagtcagc cgaaaacttt cttgtctcgg ggcgaggtga attgcatctt ggaatcctga
     1501 ttgaaacgat gaggcgtgaa gggtatgaat tccaggtgtc tcgtcctgaa gtgatcttcc
     1561 acaaagcgga tgatggcgct ctgctcgaac cgtatgaaga agtgcatgtc gaaacaagca
     1621 gtgaaaccgt gggtgttgtg gtggaaatgc tcgggagtcg acgcggtcag atgatggata
     1681 tgcaggactc aggtcagggc acgacgcgta tggtctatgt ggttccgacg cgcggcttgt
     1741 tggggttccg ttatcagttc ctcacctcca cgcgcggtgc aggagtcatg cacacgatct
     1801 ttcatggtta cgacgaaatg gctggaccca tgagcacgcg acaatctggt tcgctggttg
     1861 cctgggaggc aggtacgacg actgcgtatg ccttgaaatc agcagaagaa cgcggtgtgt
     1921 tattttatgg tccgggtgtg gatgtttatg cgggtatggt tgtcggtgaa aacgcacgct
     1981 ctggcgacat tactgtgaat gtctgcaaga aaaagcatct tacaaatgtc cgtgcctcgc
     2041 gcggagatat ggaaattcgt cttacgccac ctcggcagat gtctctcgat gaagccatcg
     2101 aatacctctc agatgatgaa ctgctcgaga tcacaccgga ggcctatcgc atccgcaagc
     2161 gaattcttaa ttcagatgat cgcggcaagc agacgaaaaa agttaaagag ttgatggaag
     2221 aataaaatag agcgaccggt taggtcgctc tattttattt aacacgtcat gcgcatgacg
     2281 gagctattgg ctgtgccaaa cgggctaaat gcctgcaagt agtattctgc attctcacca
     2341 gcgagccggg caaatgaatc agtataagtg gaggagttgg cggggatttc tgcaaccgcc
     2401 tcgccatttc ggaagatgcg gtaccctgtc tcattactgg atttatctat ccagcttaca
     2461 gtgaaagtca atattccacc gctgcaagaa aactcccatt cctgaatgga gggtgcctga
     2521 ggcggcgcct gtgtggatgt cggcggcggt gtgacgcttg agactgccga gaaactgccg
     2581 ctgagttcta catattcacc ccacaaccag cagttaccgg ttgggctttc ctcggccttc
     2641 acgagccagt agttgtccgg gtcatatctg ccaaggattt caagttcctt tcccggcaga
     2701 tacgtgaaca ggatttcata atcctgcccg ggaccagacc ggcagttggt ctgctcgatc
     2761 acggtgagca tgggcacgga atatgtcggt gttcgagtga ctgcaccagt tggcgttgcc
     2821 gtggccccag gggcgatatc ggtgggaact tctgttgcag attgctgagt gggtgttgag
     2881 tcagagggtg taaagattgc ctgcagggtt tcagccgcgg cagtttgaat ttcagcgtct
     2941 gtgggctgct gcgcattcgt gaatgggccg ttgcaggcaa gagcggcaag aagaaggact
     3001 gttcctgcca gcagtgattt aaggttgtga gacatatctc tacccgtttc tttttgtttc
     3061 tcaaatcttt ccttttaatt ctatcatttc caaccatctg tttccttaaa cgataaaccc
     3121 ccgcagtcag ttccctcgac tgcgggggtt gtttttctac cagaaatcaa ctagcgatcg
     3181 tgcgccttaa cggcactttt agttgggctc agactcatcg tgatgatcat ggagtccgcc
     3241 gccacccccg gagcccgtag cacccgcaac gactggatct accaagggat tcttctcgaa
     3301 atttcgcttt gagaagagaa gcacagagtg agccaccgca tcagacattt gatccaatgc
     3361 agtgttgctg ttgttatcaa aggtgtcaca tgccaggtga tagcagggat cgtactgttg
     3421 cccggcagtg ccgccgtaga ccgcagcctg tgccggcgtt ttgatgccct ctgcaccggt
     3481 gaacaagccg ccagccggaa tgtcaactcc cggagcgata aacggtccat aatctgaacg
     3541 tccgttgaaa ggagttggtt cggtggccag tccctgtgaa gcgaagtact ccaggaacat
     3601 ttgctcgatg gcgcctgagc ctgtaggacc cacagctcca ccgccgccgg aattgttacc
     3661 gtcatagaca aaacggacaa agtttggcga accgatcata tcgaagttaa gcatcagttc
     3721 gatcttgtcg cgttccgcct ggctcagatt agcaacataa aaatttgaac caatcaaacc
     3781 tggggaaagt tcttcagccc cataccacat gaagcgcagt ttattgcggg gagtgcgtcc
     3841 ctgggatgcg aatacttcgg caacttcaag gatggtggct gaaccgctgc cgttgtcgtt
     3901 gatgccgggt ccacgcgtga cgctatccag gtgcgcgcca actacgataa ctcgattggg
     3961 gtcgcctgtg ggggtctcag cgatcacatt acgagtggta cgcggcccaa agaaataatc
     4021 cactttaagt cgaacggtga ttcccgtagg accattcaag acaccatttc gcagcgtttg
     4081 ccccatagca aaggtggttg tgatggaagg aatggtttgt ggtgtaccga gtgttcccag
     4141 gtttaaattc gttcggccgg gattaccttc gttgaagacg atcacaccaa cggcacccgc
     4201 ggcttgcgca ttttgggctt tgtttacaaa accacaggta ccgcgctgca tcagcgcgat
     4261 atgacctggt gtaaacccgg caaagtctgc tgcctcacag ccactggtgg aggcattcgc
     4321 aaacggactc ggaacgagca agtccacggc tgtaagcgga gcggtgaagt ctccactacc
     4381 tgaaaattgc atggtgtcgt agtcgacgcc gtcgatataa actgtaggtg tgggggatgt
     4441 ttgctcgaga gcgggtgctg aggcgtcacc gtcaaaattg aaggtaaatt cctgaaagct
     4501 gacgttgtaa ccggccgcaa ccatgcgatc gtaaacatac tgcgcagagg cgtcaaaccc
     4561 cgcgctgcct gagacacggt tgccgccatt gtcattcgag atcgcctgta aagctgcctg
     4621 gtgttcgcga attcctgcca gcgtgacgtg tttgcggaaa ccctgggttt cgctgatatc
     4681 atctgccaat acaaattgag ccggtagtgc aagtgccaga gtcaatatca acacaataaa
     4741 gcggttcatt cgtttattca tgcctaactc ctcattgaaa gtcgttcggt ggggatttgt
     4801 ctgtcgagtt gattgccaat cagagaagta aaataagaaa tgcgagccga gaaatccatc
     4861 tcctttcgat aaacaaaatt accgaaggga aagtcatctg acagcctgaa aatccatagt
     4921 atttgaaaat ttatatgaca cccttctgtg aaatcgggaa ccttcggtat atttcattat
     4981 aggttgcgcc tctgagtttg caggaatacc gtagaggggt taatcacaga ccattcgagg
     5041 gtgatgcatt gaaaattctc aacccctctc acactcaata aaaaaataac ccgtactcgt
     5101 cgagtacggg ttatttttca ctaaactaaa atcagtttat tcagcgggta gttcgtggtc
     5161 gtgaccgctc tgggttgatg tactggtgtt ggaggaccca ccgccgctta ctggcgggaa
     5221 gttacctttg ccctgtgcat cgttaatatc ctgagtgctc attgcatact gtaagattga
     5281 aaatgcaacg gcatcggagt taacatcgag cgcaaaatca ttgttgttag caaacgtatc
     5341 gcaggcgagg tgataacacg gatcgtattg ttgaccagct gtgccacccc aaagcgcaac
     5401 ttcagccgct gtcttgattc cttccgcccc ggtgaagaga ccgccagatg ggatatccac
     5461 accagccgcg atgaacggac cgtaatcgga gcgaccgctg aagtcggtgc ccttgaatgg
     5521 aatgccgcgc atattgaaga aagcctcgaa ggtcttctca atttcggctg aaccggcagg
     5581 acctgcagga gaaccgacgc cgtctgaatt atctccgtca tagatgaaga agacatgatt
     5641 cggtgagccg atcatatcga agttcagata gagggtgatg cgatccaact ctgcctgact
     5701 caaaccagcg acgtattgcg tcgagccgat caagccagac tcttcagcac cccaccaggc
     5761 aaaacgcact ttattgcgcg gggtgacctt ggacatctgc tctgcgactt cgatcaaagc
     5821 agctgaaccg gagccgttgt cattgatgcc gggtcctgcc tggacggaat cgagatgcgc
     5881 acccgccatc actacattat tcgggtcacc accttgtgat tcagcaagta catttacctg
     5941 cgtcacattt cgaggtgctt caacgacaac gcgcgccgta gaaccaggtt gagccaggga
     6001 ttcaccggct gcaaatgaac ctccaaccac aggaatcgtc acagggccgg gagtgcccgc
     6061 cgcaacaaca agtgtaccta caatcaacgt cgatcgagca ggagtatcgc cctgattgaa
     6121 gagaaccatc gcacttgcgc cagcagcctg agcgttatat gccttaaggg caaaggaaca
     6181 gccacctcgc tgaatcagcg caatattgcc agccgggaag ccggcgaaat ccgttggact
     6241 gcctggggcc gcagggacga tcgcggcgcc aactgcagct tccgtatagg ctccctggca
     6301 tccgctggtg ttggcgcgcg gaggcacgag gttaatatca acgggagtga cgctcgcagt
     6361 aacagcgcca acgcctgaag ctgtgaaaac tccagattca taggctgcgt tgactggggt
     6421 cagctgttgt aaaacagcag gggggaagaa taaaaatggg aactcgtcga gcgtgacgtt
     6481 atagccagcg gctgtcaatc gctcgactac atatgccaca cttgcttcat atccgggtgt
     6541 accggctgca cgggtgcctc cattgtcatt ggcaatcgcc tggaaggcgg cttgatgttc
     6601 gcgcacccct tccagggtga cacattccaa aagcttcgcg tgggtattgt tgttgcggtt
     6661 accgcaggat gctggtcccg ccattgctac tggagcaaat gaaaaagcag ctgcgatgat
     6721 actgaccaaa gctacaatac ggattgtttt gcttaacatt gtttctcctc aaaaacgaat
     6781 gttgagattt gtgtttcgct acgcacgaac acaatcaggt cgaacaagcg atcattgaat
     6841 ctaacgcgat cacctccttt tgcttgagtg aatgctgaac ctgttctgat ctactcaaaa
     6901 gtagttcatg tcctcgcgct ccaccattgg gtggaactgt tgctgcgatt tctggttgac
     6961 gatgccccag gttgccagat tatagcgact tgacaactga tttcaaataa gagtttattt
     7021 aactacctaa actcataatg aaattttctt taggaaatat gattcataaa ttaatttgcg
     7081 gttaacgtag gctgaatcaa tctggaaata ttataccgat tttaagcggt aattcaaata
     7141 ttaaaatgtt acactgcgcg aaccagcact ataaagaagt gactacggct atggcaatag
     7201 gtggctcttt gttgatattt acccctgata tacctctccc agcttgaagg agtacctgcc
     7261 ttttacatcg taatcaactc gttgataatg gtggttggcg tgggaattgt ggacaaatgt
     7321 aatgaacatg acattgatga ataagccaat gaatgtgatt cctggtttca tctccatttg
     7381 ccgatcaagg gattcaattg aatattgatt tttaggaggg gaacatctac aatcgcaaca
     7441 tagtgctcaa tctagcggtt gaaatcctat ttaggtttgt tttatgaagg gacctaccgg
     7501 caaaatgcca taccttgatg cagtatcgcg aagtgtcgta aacggccggg catgacaagc
     7561 tggcaactgg tagtactttc agcatggagt gcccaggcgg cctggtgtag aataagaaaa
     7621 aaattcaatc gcttggaatc catatgtctg aatcaccaag acccagacca agacttgatc
     7681 cctccatcat tgcagcacta atcgggctcg taggcacgat gtttgttgca atcatgacct
     7741 atatggcgaa tcgaccggct tctccacagg caactcctat acctacagtt gctgtggtgg
     7801 aacccggtac agaaatgcca actttgatcg acactgttga ggtctctaca cagccgatgg
     7861 ctgcctctcc tgcaacggtg ccaacagtga aatacccaaa tggcaaactc ttcaaacttt
     7921 tttatgatga caacagtttc tatctgctca acatgtctga cacggcgatc cccatcaatc
     7981 gggtggcgtt cgagcggcta agtgatgcgg acgtaccatt aaatcgcttc actggcactc
     8041 gctgggccga gttttacccg gatagtactc ccggtaaatg tgtcgcgctg gaaatctttg
     8101 gcagccctcc atatcttgac cctccagaat gtggcggcgg tgtctttctc agtttgcgca
     8161 ctcctccacg cgatgatcct gctatcttct ggacggcgca ggagggaagt caccagtttc
     8221 gtgtgttgtg gcgtgaaggc ggagaggatg aagaggtggc ccgctgtgaa atcggtgcgg
     8281 taacttgcgc ggtgttttta ccgtgaccgt gtgattaggg gtgtaaatct ttctcttcat
     8341 caacaagcta ctccatttga cttaaagaag tccttcgttc acaatgtcag cacatcctga
     8401 aaggatgatt gaaaagggct tatcatgctt cttcttgata ttgtaaaggc tccgaaaaat
     8461 ggcaagtatt aaaactcttg aggttcccat caggggtatg gattgcgttg agtgtacaca
     8521 acacgtagga aatgcaattt ccaaacttga gggtgtgaaa tctgttgatg tccttcttgc
     8581 agcagagaag gcaattatcc aacttgaccc tgaaaaagtc gatatgtttt ccattcgcga
     8641 ggctgtagcc agtgcaggag atgcctattc cgtccctgaa acagtagaag cggttcagcc
     8701 cctctcgatc agcgatttta atagccaatt aacgaagatg ctcgttgttg tattcagcat
     8761 cgttttctcc attgttatcc tgggtgaatg gctgggtgtt ttcgaattgc tcgctgcttt
     8821 tattcccttc cccgtgggtt ttgggatcgt gctcctcgca gggtggcccg tatttcgtaa
     8881 agtttttatt gctactctaa agcgtcagat actgtctcat acgctgatga ccctcggtgt
     8941 gatcgctgca ctcgcagttg gggagtgggt cactgcggcg attgtcgtca tctttatgcg
     9001 cgtgggtgag tatgtcgaaa acttcaccac agaaggtgcc cggcgcgcgg taaaagaact
     9061 aatcgcgatt gctccgcaga cggctcgcct ggagcgtgac ggtgcggaaa ttgaagtgcc
     9121 tctggcagaa gtcagggttg gcgaaattgt gattgtccgc cccggcgaac ggatacctgt
     9181 ggatggagaa gtgctgagcg gtcacgccac aattgaccag gccgcgatta ccggcgaatc
     9241 aatgcccatt gaagcaaaaa atggcacata cgtgtttgcc gcaacgattg caaaacttgg
     9301 gagcctgcga gtcaaaacga tgcgaatcgg cgcagacacg acgttcggac gtatcatcaa
     9361 actggtggaa gaggcggaag caaaccgggc tgtggtgcaa cgatttgcag ataagttcag
     9421 tgcatattat ctacctgtgg tcgcagggat cggcgcgctt acgttccttc tcagccgtaa
     9481 tccattatca accgcagctg tgctggtggt ggcgtgttcc tgttcgattg ccttagcaac
     9541 accaatcgca atgctggctt ccattggagc aagtgcgaaa cgcgggctac tgatcaaagg
     9601 cggtaaatac cttgaagctc tcgcccaggc ggacgttatt ctggtggata aaaccggcac
     9661 gctgacgctt ggtcagccac agattacgga tgttattcct ctcaatggat tgcccccttc
     9721 agaaatcctt gccattgcag cgtctgccga acgctactct gaacacccac tggcacaagc
     9781 agtgcgagat gcagcgttga aggaccagtt gaggttgtct gagccactgg attttgaagc
     9841 gattcccggg caaggcattc gggcaagaat caacggcaaa tccgttgcgg tcggcaatcg
     9901 acgtctcatc ccgacagctg cctccttgcc catcgctgct caactggaag aacaaggcaa
     9961 aacactattg tttttggaac tggataacga gtcggcgggt attttagcgg ctgccgatac
    10021 acttcgggct gaagtcccag cagcttttgc cgagctacgt agatttggaa tcaaacatat
    10081 tcaactccta accggtgata ataaacgtac ggcgtctgcc cttgctgaaa aactgggtgt
    10141 tgaatatcga gcaaatcttc tccctgaaca taaaattcaa atcgtaaaag aatatcaatc
    10201 caaagggcac acggtggtca tggtaggtga tggagtgaat gacgctcccg cgctggcaca
    10261 agccgatatt ggtattgcaa tgggcgcggc aggcaccgat attgccatcg aagctgcgca
    10321 tattgctctg atgcgcgaag attggtcact ggtgccggag gctttataca tcgcgcgccg
    10381 caccatgggc attgtaaaaa tgaacctggc gtttactgct ctgtataacg taattgggtt
    10441 agggttggcg gcatttggct ttttgccacc cgtctttgct gctgctgcac aatcaattcc
    10501 agatgtcggt atcctcgcaa actcggcccg tctgttaaat caaaagaaaa accaacaaat
    10561 ttgaatagcc ggatttatca tggtacgaga ctaattgcaa aacatgaatt aacgctgatg
    10621 gtttactgag atgattctga ctactgtgca aaacgacggg ccgtggtgcc cgtcgtttgt
    10681 tttaatatcg
//
LOCUS       DASTJP010000656         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1216017, whole genome shotgun sequence.
ACCESSION   DASTJP010000656 DASTJP010000000
VERSION     DASTJP010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216017"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFQ23_19215"
     CDS             <1..317
                     /locus_tag="VFQ23_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908788.1"
                     /translation="AAKSQLNLSAQAYHQSADPSLSQAQSNSLGGPFPARSLLSGELP
                     SKNYFPRKTLHTQFWFLIQSYIKASYRRQRYAVHKPAQPVGAGVSLNPEAGVYNCRSE
                     GV"
     gene            complement(456..2171)
                     /locus_tag="VFQ23_19220"
     CDS             complement(456..2171)
                     /locus_tag="VFQ23_19220"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9908789.1"
                     /translation="MKATSRLDKIVSLIDEHSFLTVSDLSRLCDVSEMTIRRDLDHLQ
                     KQNRILRTFGGAVPVRTESFPDDSAIPPHDQRSEVLLVDQVDVLIGTSVNPYYDNLLI
                     DRAIKKNIAIIAESIEMPNQRTVVAVDNYQGAYDLGSWTGNYLKQQSVKEVNLLDLTF
                     HQPNTQNRSRGFADGLNRTSPSFQTILSINAQSRYATAYQLSRDALIVHPQINLIFAI
                     NDTTAWGAINACRDLNIDPGQMTVVTFGLEGETLKNELMSQDSYCKGGLAMFPEIVSL
                     TCIEAAIAAFNHQRQPDKYVTPHVVLTAETLPGYYKRTASGWQLDWEIVRNNLNIPIK
                     IEWGKPDPETKLPKQIGLIVPFSEHEWYKNLKVLLKEYSEQYGIGLQIIDADQNVRDE
                     VELRRRQIASKAATLVNHGDVVLIDGGPIAPYLAKELKQKKEITVITNSVFVFDTLNR
                     TPDITLISTGGAVRYSTQMLVGPTAEGALKELRADKLFLMVSGISLDFGLSHHTISEV
                     TMKQAMIRSAREVILLADHTAFGAEVGIQVAPLKAIHKLITDDALPPSIRLDISKAGI
                     LITLA"
     gene            2511..>2782
                     /locus_tag="VFQ23_19225"
     CDS             2511..>2782
                     /locus_tag="VFQ23_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908790.1"
                     /translation="MYSKKILSVMGLIAILAMLMTACGAGQTPVEGEKTLTIAGVVFQ
                     DDQFMKSMVQGYEDAGAKYGVKVMTANTNNDQAKEAELIQTYIAQGV"
BASE COUNT          726 a          646 c          648 g          762 t
ORIGIN      
        1 gggctgcaaa gagccagctc aatctttcag cgcaggcgta ccaccagtcc gctgatcctt
       61 cattatcgca ggctcaatct aattctcttg gggggccttt tccggcaaga tcactccttt
      121 cgggagagtt accatctaaa aactatttcc cccgcaagac gctgcacact caattttggt
      181 tcctgatcca gtcctacatc aaagcctcat acaggcggca acgttatgct gtgcataagc
      241 cagctcaacc tgtcggcgcg ggcgtatcat tgaatcctga agctggcgtc tataattgca
      301 gatctgaggg agtgtgaaga aatttgatcc gtccatttgg cggaggcatt gcaaggtcgt
      361 tcaaagtcga ttcagggata gcatgggttc tcataacaag aggtatttca tcaaatgaac
      421 gccgcttctc cttgaagaga aaccaggttc atttcctatg ccagcgtaat caggatacct
      481 gccttcgaaa tatccaatcg gatactcggt ggcagtgcat catcagtgat cagtttatga
      541 attgctttca gcggggcaac ttgaatccct acctcagcac caaaagcagt gtgatctgcc
      601 agtaagatca cctcgcgcgc agagcggatc atagcctgct tcatggtaac ttcagagatg
      661 gtgtggtgcg acaacccaaa gtccagtgaa atcccactca ccatgagaaa tagtttatcg
      721 gctcgtaatt ccttgagtgc tccctcggcc gtgggtccca ccaacatctg tgtactgtaa
      781 cgcactgcac cgcccgtgga aatcagagtg atatctggag tccggttcag tgtatcgaat
      841 acaaacacgg aattggtgat cacagtgatt tccttttttt gtttcaactc cttggccagg
      901 tagggagcga tcggccctcc gtcgattagt acaacatcac cgtggtttac aagggtagct
      961 gccttgctcg caatttgtcg gcggcgcagc tccacctcgt cgcgaacatt ttggtcggca
     1021 tcgataatct gtaatccgat cccatattgt tcagaatatt cttttagtag taccttcagg
     1081 tttttatacc attcgtgctc actgaaaggt acgatcaatc cgatttgctt gggtagtttg
     1141 gtctcaggat caggttttcc ccattcgatc ttaatgggga tatttagatt atttcgaaca
     1201 atttcccaat ccaattgcca tcccgaagca gttcgtttgt aatacccggg taaagtttcg
     1261 gccgttaaga caacgtgagg ggtaacgtat ttatctggct gtcgctggtg attgaatgca
     1321 gcaatggccg cttcaatgca ggttaggctt acgatttcag ggaacatagc cagtccaccc
     1381 ttgcagtaac tgtcctgact catcaactca ttttttagtg tttcaccttc cagcccaaat
     1441 gtaacgacgg tcatttgccc cgggtcgatg tttagatcac gacaggcatt gattgctccc
     1501 caggcagtcg tgtcattgat ggcaaagatc aggttgatct gtgggtgcac aattagcgcg
     1561 tccctggaga gttgatacgc cgtggcatag cgggattggg cattgatgga aaggatggtt
     1621 tgaaatgagg ggcttgtcct gttcaggccg tcagcgaagc cgcgactacg gttttgggta
     1681 tttggttgat ggaatgttaa gtccagtaaa ttaacttcct taacactctg ctgcttcaga
     1741 taattgccgg tccagctgcc caggtcatat gccccctggt agttatcaac ggccacgaca
     1801 gttcgttgat tgggcatctc tatagattca gcaatgatgg ctatgttctt ttttatagcc
     1861 ctgtcaatca gcaggttatc gtaataggga ttgacggatg taccaatcaa tacatctacc
     1921 tgatctacca acagaacctc agacctttga tcatggggag gtatcgcact atcgtcagga
     1981 aacgattcgg tccgtactgg cacggctcca ccaaaagttc ggagaatgcg attctgtttt
     2041 tgaagatgat caagatcccg gcggattgtc atttctgaca catcgcagag gcggctcagg
     2101 tcactcaccg tcaggaagct atgttcatcg atgagtgaaa cgatcttatc aaggcgtgaa
     2161 gtggctttca taattagcat ccttgttcaa aaaagatgaa tcacaaagac attatactca
     2221 agtaatgtta aatgtcaaac aaaacttaac gatactcgaa cattaatact tgactttaat
     2281 tcctgattgt gctaatatgc acaagtaggt gctgattttt atccggaatc acaaaaaaac
     2341 gagtattttg gtaatctata aacatttatt ggtgggaatt gtttgaatag ctattcaatt
     2401 cgctatatgc cttatagaga aggagctgac aagatatcaa atattaaata cccctttccc
     2461 tttctacaat tcaagacctt ccactttgta ctcaatttaa ggagaagaaa atgtacagca
     2521 aaaaaatcct ttcagttatg gggctcatcg cgattctggc aatgctcatg acagcctgtg
     2581 gcgcagggca gactccggta gaaggtgaaa agacactcac aattgcaggt gttgttttcc
     2641 aggatgacca gttcatgaag agcatggtcc agggctatga ggatgcaggc gccaagtatg
     2701 gcgtcaaagt catgaccgcc aacaccaaca atgaccaggc gaaagaagcc gagctcatcc
     2761 agacctatat tgcccagggc gt
//
LOCUS       DASTJP010000657         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1078001, whole genome shotgun sequence.
ACCESSION   DASTJP010000657 DASTJP010000000
VERSION     DASTJP010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078001"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1414
                     /locus_tag="VFQ23_19230"
     CDS             89..1414
                     /locus_tag="VFQ23_19230"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET9908791.1"
                     /translation="MPQATYHFPRGFLWGTATAAHQVEGNNTNNQWWLWEQNGHTKGT
                     SGLAADWWGGRWREDFDRAAEAGQNAHRLSVEWSRIQPTPDTWDEDALERYRTMLRGL
                     RERGMTPMITLHHFSDPLWFFEMDGWEIDESVALFEKFVRKTVEALKEYCTLWCTVNE
                     PNIYSLSGYVVGDFPTKHRGTNYAMQVQANLIRGHAAAYRAIHEIQKEARVGYALHYR
                     PMVAAHAWSPLDVLMRKIRYDGLNMAFPSALSTGVMKSPVTRVNIPEAKGTQDFLGIN
                     YYSVDTVSFHLGKRNQLFTHSEFPKDADLSDTKFVANIPEGLFDTIKWAGRTYPNLPI
                     IITENGVEDADDHMRPRYLAQHVHQMWRAVNFNWPVKGYFHWTLVDNFEWERGWTQRF
                     GLWALDPETQKRTKRPSADLYAEICRENGLSSEMVQKYCPEVFDKLFPV"
     gene            1423..>2206
                     /locus_tag="VFQ23_19235"
     CDS             1423..>2206
                     /locus_tag="VFQ23_19235"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9908792.1"
                     /translation="MNLPTDFVSTITNTFGEDGKSLIANLPNLIEGASSRWGLINIQP
                     VENLSYNFVAFADSPLPVGEGLGVRAKQNVVLKIGVPRDELTSEMAALRLFNGDGACR
                     LIDADEEKGLLLLERLHPGVMLSTMEDDEQATHIGAEVMQKIWKTGFDTPSAEGYSTD
                     GSRFIQLSDWFDGLKKLRDMFNGGTGPLNEKLVDRVEQSTKDFFVENHQPVLMHGDFH
                     HFNILSSERGWLVIDPKGVVGPAGYEVGPFLINPWGEMSNEIN"
BASE COUNT          558 a          518 c          592 g          538 t
ORIGIN      
        1 ccgggtcagt cgtattgtat ttggcagatt tggcaagaaa tccgtaggga cgggcagctg
       61 cccgtcccta catcatatgg agacataaat gcctcaggca acttatcatt tcccacgcgg
      121 gtttttatgg gggacagcaa ccgcagcaca tcaagtggaa gggaataaca caaataatca
      181 atggtggttg tgggaacaga acggtcatac caaagggacc tctggcctgg ccgccgattg
      241 gtggggtggt cgctggcgtg aagactttga tcgagctgcc gaagccgggc agaacgcgca
      301 tcggctttcg gtggagtgga gccgtattca accgacgccg gatacctggg atgaagacgc
      361 actggagcgt taccgcacca tgctacgcgg cttacgcgag cgcggcatga cgcccatgat
      421 cacgctgcat catttctccg acccgctgtg gttcttcgaa atggatggct gggaaattga
      481 tgagtcggtt gccttgttcg aaaaatttgt tcgtaagacc gttgaagcat tgaaagaata
      541 ttgtacgctt tggtgtaccg tcaacgaacc caatatatat tcattgagtg gttatgtagt
      601 cggtgatttc ccaaccaaac accgcggaac caactatgcc atgcaggtgc aggcaaatct
      661 gattcgaggg catgctgctg cctatcgcgc catccatgaa attcaaaaag aggctcgtgt
      721 gggttatgct ttgcattatc gtccgatggt cgctgctcat gcctggtctc cgctggacgt
      781 tttgatgcga aagattcggt acgatggatt gaacatggcg tttcccagcg cacttagcac
      841 gggtgtcatg aaatctcctg tgacaagggt aaacatccct gaagccaaag gcacacagga
      901 ctttcttggc atcaattatt actcagtaga tacagtcagt ttccatcttg gcaaacggaa
      961 ccagttgttc acacatagcg aatttccgaa agatgccgat ttgagtgaca caaaatttgt
     1021 cgccaacatt cccgaaggct tatttgacac catcaaatgg gccgggcgaa cgtatcccaa
     1081 ccttccaatt attatcacgg agaacggtgt ggaagatgcc gatgaccata tgcgaccgcg
     1141 ttatctggca caacacgttc atcagatgtg gcgcgcggtt aatttcaact ggcccgtgaa
     1201 gggatacttc cattggacgc tggtggataa cttcgagtgg gaacggggct ggacgcagcg
     1261 ctttggtctt tgggcgttgg atcctgaaac acaaaagcgc accaagcgcc ccagcgccga
     1321 cctctatgcg gagatctgcc gagagaatgg actctcctct gagatggtgc agaagtattg
     1381 cccagaagtc ttcgacaagc tgttccccgt gtgacgttaa taatgaatct tcctactgat
     1441 tttgtttcaa cgatcacgaa tacctttggc gaagacggga aatccttgat tgcaaatctc
     1501 ccaaatctaa ttgaaggagc ttcatcgcgc tggggactca taaatatcca gcctgttgaa
     1561 aatctctcct acaacttcgt ggccttcgca gattcccctc tcccagtggg agaggggcta
     1621 ggggtgaggg cgaagcaaaa tgtcgtcctc aaaatcggtg tgccgcgcga tgaactgacc
     1681 agcgaaatgg cagcgctacg tctctttaat ggagatggtg cctgtcgctt gatcgatgca
     1741 gatgaggaaa agggacttct attgctggaa cgattgcatc ccggcgtgat gctgtcgaca
     1801 atggaagatg acgaacaggc aactcatatt ggtgccgaag tcatgcaaaa gatttggaag
     1861 actggtttcg atacgccatc tgctgaaggt tattccaccg acggttcaag atttattcaa
     1921 ctctcggatt ggtttgatgg gctgaaaaaa ctacgagaca tgttcaacgg cggaacaggt
     1981 ccattgaatg aaaagcttgt agatcgagtg gagcaatcta cgaaagattt ctttgtagag
     2041 aatcatcagc ccgtgcttat gcacggcgat tttcaccact tcaacatttt atcttccgaa
     2101 cgcggctggc tggtgatcga cccgaaagga gtagttggtc ctgcgggcta tgaggtgggt
     2161 ccgtttctca tcaacccgtg gggagagatg tcaaatgaaa tcaatc
//
LOCUS       DASTJP010000658         4135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1005576, whole genome shotgun sequence.
ACCESSION   DASTJP010000658 DASTJP010000000
VERSION     DASTJP010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005576"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFQ23_19240"
     CDS             <1..275
                     /locus_tag="VFQ23_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908793.1"
                     /translation="YTLLKRLVDRYESNSHYELESLPKDFVEKEIKGIVAYQIEVTKI
                     EANYKLSQNRKDEDYANIITQLELRTDEFSHGVAAAMRQNRPEISR"
     gene            446..967
                     /locus_tag="VFQ23_19245"
     CDS             446..967
                     /locus_tag="VFQ23_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908794.1"
                     /translation="MIEPEELSEYESSQEKEIFQSSVLSVTADAVRMHQADAETITAD
                     IVELTQSAAANVKATQISAHQSALAAVNAAEVVSHNSANAFIQAEKASIGGVTGAVLA
                     GTAEIRNGVTGFVVGQDVHVTEGRTIFLLSRNLYGNVTTLMDSRAALTAGLVAGLFGG
                     LMMLLGQMLVRRR"
     gene            1130..1795
                     /locus_tag="VFQ23_19250"
     CDS             1130..1795
                     /locus_tag="VFQ23_19250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET9908795.1"
                     /translation="MMEKVVLKATKRDVIGKQVKAMRREGKLPAVIYGRHNEPIIISL
                     DSHTASLALGRLSSSSLVTIDVDGTEYATLVREKQRDFIKNRLLHVDFLAVSQDETLT
                     ATVSLHFVGVSIAVKDYNAVLVHNLEELEVECLPADLPERIDVDISVLSRIGDGIRVR
                     DVVVSDKVRILENPDTMVAVATAPKVEEEGAAVPGAEGVTPTEAEPELSVERGKKEDE
                     EEE"
     gene            complement(1968..2570)
                     /locus_tag="VFQ23_19255"
     CDS             complement(1968..2570)
                     /locus_tag="VFQ23_19255"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET9908796.1"
                     /translation="MSINLTEHDIAQRLQAAIGSAGPSSDGYAELELNDEMTLKCAAV
                     LVPLVWHEEEWHLLFTRRTDRVESHKGQVSFPGGACDEGETTPEQTALREADEEIGLQ
                     PAEVKVLGRLANLITISYFRVTPVVGVVKWPTVFRVGEHEVARVFTIPLVWLANPSNR
                     WQFEIPGRSRSVIAYHPYDGELLWGATARMTVDFLKVLGL"
     gene            complement(2624..3163)
                     /locus_tag="VFQ23_19260"
     CDS             complement(2624..3163)
                     /locus_tag="VFQ23_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908797.1"
                     /translation="MRRRFRREIRKKLAASVPPVLQEAHYAFNKGEYGRAGELFEQIA
                     ETADARGGPRAALFCLQAGRARVLAGQSHLGMPSLKRGLEILSQRKLFPQLQKAGSRV
                     IAELTEQGLKNEAAEIEMWLKSILLLEPAFETGIASEKRPVLPTHCPSCGAGLRSDEV
                     EWIDSVTAECAYCGSPVRS"
     gene            complement(3181..4020)
                     /locus_tag="VFQ23_19265"
     CDS             complement(3181..4020)
                     /locus_tag="VFQ23_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HET9908798.1"
                     /translation="MILTPTSSIARDGDLAQLVGLRHKHFILSLQAGSKFETHRGIIQ
                     HNELIGKPWGTQVFSHLGSPFFLLQPSLADLLIDLPRTTQILYPKDIGFILVTMGVGH
                     GQTVLEAGTGSGSMTTALAYSVGPAGRVISYEVKPDVQNLARKNLSRFGLESRVDFKL
                     RDIQLGFDETDVDSFFLDVPNPYDYVRQVRAALKSGGFLGCLVPTFNQIEKTLQALYQ
                     TNFAFIEVCEILLRYFKPEPARIRPTDRMVAHTGFLIFARRIEPSEDPRGKELSVEIG
                     ADR"
BASE COUNT         1050 a         1042 c         1093 g          950 t
ORIGIN      
        1 cttatacatt actaaagcga cttgttgatc gatacgaatc gaacagtcac tatgaactgg
       61 aatcccttcc aaaagatttc gtcgagaaag aaatcaaagg tattgttgca tatcaaattg
      121 aagtgacaaa aattgaagcg aattacaaac tcagccagaa ccgcaaagac gaagattacg
      181 ccaacatcat cactcagctt gaactgcgaa cggatgagtt ctcgcacggc gtcgcggcag
      241 cgatgcggca aaaccgccct gaaatctctc gctgattgta gacaacgcgc gcttcgtcta
      301 cggtctttgc cacgaacgct cagatctccg ctcagcgcga agtttgtggt aaaatccgag
      361 ccgttatacc ctccgcctcc tgctggaggc ggattttgcg ccagcactgt ttcattggta
      421 cgctagtagt gaatgagaaa tcaaaatgat cgagcctgaa gaactctcag aatacgaatc
      481 atcacaagag aaagaaatct ttcaatcatc tgtgttgtcg gtcactgctg atgccgtgcg
      541 gatgcatcaa gctgatgccg agaccattac agccgatatc gttgaactga cacaaagtgc
      601 ggctgcaaac gtgaaagcca cacagatcag cgcgcaccag tctgcactgg cagcggtaaa
      661 cgcagcagaa gtcgtttcgc ataacagcgc gaacgccttt atccaggcgg agaaagcatc
      721 cattggcggc gtcaccggcg cggttctggc gggaactgca gagatccgaa acggagtcac
      781 aggctttgtt gtggggcagg atgtccatgt aactgaggga cggacgatat tccttctctc
      841 ccgcaatttg tatgggaacg taacaaccct gatggactca cgagctgctt tgactgccgg
      901 gttggtggca ggtctcttcg gtggtttgat gatgttgctt ggacagatgc tggtccggcg
      961 aagataatta tttgaatgta gtagcgactt tgcctggcac cctttcgggt aagtcgctta
     1021 attacgacga ctgacccaca gggtgcttcg caaggtcgtt gctacaaaaa ttctattcaa
     1081 cgagcgaagg taatctatcc gatgataggt gagaggcgct caaggagtaa tgatggagaa
     1141 agtagtttta aaagcaacca agcgggatgt gatcggcaaa caagtcaaag ccatgcgccg
     1201 cgaaggcaaa ctgccggctg tgatctacgg caggcataat gaaccgatta ttatatcttt
     1261 ggactcccat acggcttcac tggcattggg gcgcttgagt tcatccagcc tggtgaccat
     1321 tgacgtcgat ggtacagagt atgccacgct cgtccgtgag aagcagcgtg atttcatcaa
     1381 gaaccgcctc ctgcacgtgg actttttggc tgtttcacag gatgagacac tgacggcaac
     1441 cgtgagcctg cacttcgttg gtgtatccat cgcggtgaaa gattacaacg ctgtgctggt
     1501 acacaacctc gaagaactgg aagtggaatg tcttcccgct gacctgccgg aacgcattga
     1561 tgtggatatt tctgtccttt cccgtatcgg tgacggtatc cgtgtgaggg atgtggttgt
     1621 ctctgacaag gttcgcatcc tcgaaaaccc cgatacgatg gtcgcagtgg cgaccgcgcc
     1681 gaaggtggaa gaggaaggcg cagcagttcc tggtgccgaa ggtgttacac ccaccgaagc
     1741 cgaaccggaa ctctccgtgg aacgcggcaa gaaagaagac gaagaggaag agtaaatctc
     1801 tttcggatac gaagcgaact ccccgatttt ggtcggggag tttttatttt cctcgcgctg
     1861 agcggagcac agtgaagacg aagcgcatat aacaaaaaac acttcactct tggcgcgctt
     1921 cgtctatggt ctttgcaacc agcgctcaga ccgccgctca gcgcgaatca caatcctaac
     1981 accttcaaaa aatctacggt catacgcgcg gtcgcacccc acagtaattc accatcatag
     2041 ggatgatagg cgataacgct gcggctcctg cctggaatct cgaactgcca tcgattagac
     2101 ggattcgcca gccacaccag cggaatcgtg aacacgcggg ctacttcatg ttcgccaacc
     2161 cggaaaactg tgggccattt cacaacgcct actaccggcg tcacgcggaa ataagaaatg
     2221 gtgatcaggt ttgccaatct ccccagcacc tttacctcag cgggctgcag accgatttcc
     2281 tcatctgctt cgcgcaaagc ggtttgttcg ggcgtcgttt caccttcatc acatgcacca
     2341 cccggaaagg agacctggcc cttatgagac tcgactcgat ctgtacgccg cgtgaagagc
     2401 agatgccatt cttcttcgtg ccacacaagc ggaacgagaa cggccgcaca tttgagcgtc
     2461 atctcatcgt tgagctcaag ttcggcgtaa ccatcggagg aaggaccggc agatcctata
     2521 gctgcctgca ggcgctgggc gatatcgtgt tcggtgaggt tgatagacat tgtacagaga
     2581 ttaagtaaca atcccgcgcg gaaagcgagc ggctgtcagc ttgttatgat cttacagggc
     2641 ttccgcagta tgcacattct gcggtgacgg aatcgatcca ctccacttca tctgaccgta
     2701 agcccgcccc gcacgacgga caatgtgttg gaagaacggg gcgtttctca gaggcgattc
     2761 cggtttcgaa ggctggctct aacaatagaa tcgacttcag ccacatttca atttctgcgg
     2821 cttcattctt taatccctgc tcagtgagtt ctgcaatgac gcgcgaacca gccttctgta
     2881 attgtggaaa cagtttgcgt tgcgagagga tttccagtcc gcgttttaga gagggcatgc
     2941 ccaaatgaga ttgaccggca agaacgcgtg cacgacccgc ttgtaaacag aagagagcag
     3001 cgcgtgggcc gccgcgtgca tcagcagttt cagcgatttg ctcgaagagt tcaccagctc
     3061 gtccgtattc gcctttgttg aacgcataat gtgcttcctg tagaacaggt ggcacactcg
     3121 ctgcaagttt tttacgaatc tcacgacgga atcgacggcg cattttcgtt tcccttaata
     3181 ttaccggtca gcaccgattt caacagatag ctctttccca cgcggatctt cgctcggctc
     3241 gattcttctg gcgaagatca aaaagccggt atgggcaacc atacgatccg taggtcggat
     3301 gcgtgcaggt tcgggcttga aatatcgaag caggatttca caaacctcaa tgaaagcaaa
     3361 attagtttga tacaaggcct gtagggtttt ttcaatttgg ttgaaggtgg gcacaagaca
     3421 tccgagaaac ccaccagatt tcaacgcagc gcgtacctgt cggacatagt catacggatt
     3481 tggcacatcg aggaagaaag agtctacatc ggtttcgtcg aagccaagct gaatatctcg
     3541 caatttgaag tccacgcgcg attcgagtcc gaagcgagac aagtttttgc gcgccagatt
     3601 ctgcacatcc ggtttgactt cataggaaat gacgcgacct gcagggccca cggagtacgc
     3661 caaagcagtg gtcatcgagc cagaccctgt acctgcttcg agcacggtct gcccgtggcc
     3721 cacgcccatc gtaacgagaa taaagccaat gtctttggga taaaggattt gagtggtacg
     3781 cggcagatcg ataagtagat ctgccagtga aggttgaagc aaaaagaagg gcgagccgag
     3841 atggctgaag acctgtgtgc cccatggttt gccgatcaat tcattgtgct gaatgatacc
     3901 gcgatgcgtt tcgaattttg agcccgcttg aagtgacaga atgaaatgtt tatggcgtaa
     3961 acccacaagt tgagcaagat ccccgtcgcg cgcgatagaa gatgtgggcg tcagtatcat
     4021 gagggtatct taccataccc atcggtgctc tgtttaagcg agacgtgtca caaagtttga
     4081 tcttgcgaaa cccctgaagg atctggcgtc ggtgcaagag gatcgctgtt caggt
//
LOCUS       DASTJP010000659         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1005667, whole genome shotgun sequence.
ACCESSION   DASTJP010000659 DASTJP010000000
VERSION     DASTJP010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005667"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(594..779)
                     /locus_tag="VFQ23_19270"
     CDS             complement(594..779)
                     /locus_tag="VFQ23_19270"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9908799.1"
                     /translation="MIKNILVPLDGSMLAECVLPHVCAIAQAFNARITLLHIVTLPDE
                     GSSKLTIDAFDWLLKLV"
     gene            complement(809..1870)
                     /gene="hypE"
                     /locus_tag="VFQ23_19275"
     CDS             complement(809..1870)
                     /gene="hypE"
                     /locus_tag="VFQ23_19275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET9908800.1"
                     /translation="MNSIPLPDIELTCPLPISDYPAVLMAHGGGGRLTHQLIERMFVP
                     AFKNSLLDGRHDGVILPSHKGKLAFTTDSFVVKPLFFPGGDIGKLAITGTVNDLAMCG
                     ARPAFLSAAFIIEEGLPMATLWNIIQSMQQTAQEAGVQVVTGDTKVVDRGKGDQLFIN
                     TAGVGFTTYEGTISPQSVRPGDVLLLSGDIGRHGMAIMAAREGLAFESVIESDCAPLT
                     RLVFSLLEAGIEVHCLRDLTRGGLASALVEIAQSCGGHIQIDEVTIPVREDVRGACEI
                     LGLDPMYVANEGRFAAFVAEKDASRALSILQSQPAGAQAAVIGQVQPAKDSMVTIRSM
                     IGAHRIVDMISGEQLPRIC"
BASE COUNT          519 a          550 c          507 g          478 t
ORIGIN      
        1 actggagaaa agcctataca aacacctaca taactacccc aaccacagac cgcacatcta
       61 gtctaaaact agagtaaaat gaccccaggt agccgttttc cgattcacgg ttcgggtctg
      121 tgaggtcccg agttcaaatc tcggcacccc gacagtagtc cacatggtta gccttttagg
      181 ctaaccatgt ggacttaaat aggctgcgaa tcagggggcc tgaaactgcg gtccacatgg
      241 ctcttatccg tgaggtccgc agtcaaccaa atcagaaatt gatgcaaatt gtagtccaca
      301 tagtttttct tctttggatt gatcactgca aagttgagta ttttgaatct ccttaacgag
      361 agacaagtca tatactcttt tcgtgggcca tagaagagaa ctcaaacttc agcaagctgg
      421 gtgaggctgg caaatgccat ctattaggct tggcgcgtgt ccgcatagtg tcggaagata
      481 aaacgagaca acgttgtgaa ctgcacaacg ttgtctcaac ctgcggtccc gacgggatta
      541 gggttcgcga cgcgtaatta ctagggcgct cagtaagtta gttgtttctc aaatcatacc
      601 agtttgagca gccaatcaaa tgcgtcaatt gtcagtttac tacttccctc atctggaaga
      661 gtaacaatgt gcaaaagcgt aatgcgcgca ttaaaggcct gagcgatggc gcacacatgc
      721 ggaagcacac attccgccag catcgatcca tccaaaggaa cgagaatgtt ctttatcata
      781 gtttcctcag gctctacttg attgagtctt agcaaatacg cggaagctgt tcgccgctga
      841 tcatatctac aatgcgatga gcgccaatca tgctgcgaat cgtaaccatg ctatctttag
      901 caggttgcac ttgtccgatc acggcagcct gtgcacccgc tggctgcgac tgcaatatgg
      961 acagggcccg actagcgtct ttttcggcga caaaggctgc aaaacgccct tcgttggcaa
     1021 catacatggg atcgagtcca agaatctcac aagcaccgcg aacgtcttcg cgtacaggaa
     1081 tcgtgacttc gtcaatctgg atatgcccgc cgcaactctg ggcaatctcc actaaagcgc
     1141 tggcaagtcc accgcgggtc aggtcgcgca ggcagtggac ttcaatcccc gcctccaaca
     1201 ggctgaagac caggcgggtt agtggcgcgc aatcactctc aatgacactc tcaaaggcaa
     1261 gaccttcgcg cgccgccatg atagccatcc cgtgcctgcc gatatcgcca ctcaagagca
     1321 gcacatctcc aggtcggacg ctttggggcg aaatcgttcc ttcatacgtg gtaaagccta
     1381 caccggctgt attgatgaat agctgatcgc ctttaccgcg atccacgact ttcgtatcgc
     1441 cagtgacaac ctggacgccc gcctcttgtg cagtttgctg catcgattgg atgatattcc
     1501 acaaggtcgc cattggcagc ccttcttcga taataaaggc agcacttaaa aacgcagggc
     1561 gcgcgccgca catggccagg tcattgaccg tgcccgtaat tgctaacttg ccaatgtcac
     1621 ctccggggaa aaagagcggc ttgaccacaa aggaatcggt ggtaaaggcc aattttccct
     1681 tatgggaagg caagataacg ccatcatgcc gcccatccag gagtgaattc ttaaaggctg
     1741 gcacaaacat gcgctcgatt agctggtgcg ttagcctacc tccgccaccg tgcgccatca
     1801 atacggcagg ataatcagag atgggcaaag gacaggtcag ttcgatatcg ggtaacggaa
     1861 tagagttcat tttattctca attctcggcc cttgctttgc actgggattt tggaagcagc
     1921 gggccgtgat agagtggatg gggctgcagc ttcacacgtt gctcgtctcc atgttctcga
     1981 cccgccggta ccgataataa gcagcacacg ctccttcggt ggataccatt gtcgcaccga
     2041 gtgggtgttc aggt
//
LOCUS       DASTJP010000660         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1103859, whole genome shotgun sequence.
ACCESSION   DASTJP010000660 DASTJP010000000
VERSION     DASTJP010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103859"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFQ23_19280"
     CDS             complement(<1..963)
                     /locus_tag="VFQ23_19280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9908801.1"
                     /translation="MRRYRFLFNKIGWALFTILFVLVLNFFLFRVLPGDPARAGIRDP
                     RLKKDAVETLRVRFGLDKPVINCFETLNPIKLGDCSVNPLDTQFFIYLRNLTRGELGI
                     SFHTNRPVFDVLGERLWNTILLIGAGQILSIFVGVIFGVFAAWKARTSIDYGALITGL
                     LAWSLPTFWLGIILLFWGSNHGFPIGGKATPGISSYPLLFQVGDIARHMLLPTVTYTI
                     VYMGEYLLLMRSSLLDVLAEDYILTAKAKGLSNFQILKDHALKNAMLPIVTIIAINLG
                     FTVAGAIQIETVFSWPGLGLAIYESVGRRDFPVLQGAFLLIAISV"
     gene            complement(1046..2572)
                     /locus_tag="VFQ23_19285"
     CDS             complement(1046..2572)
                     /locus_tag="VFQ23_19285"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9908802.1"
                     /translation="MNPGTAVLSEAYTIFALVYDTIYEYQLDGTYKLDVAESADVSDD
                     GLTWTFKIRDGINFHDGQPMTANDVAFSVNLYKNNEDYIYLNAYTANFDTVEATDDST
                     VVITLTDAVPNMDYLLSYLYVVPEHIWSANAEAPASVEFENVEMIGTGPFKMAEYSQN
                     EFVRLTANKEHFAASPKIDEVVFQTFESDDVLVQSLRSGQVDMITEMPATAVETLQGD
                     TNIKVVTGAPFAPDVSDVIFNQADPENCPTDAGGLCTGHPALRDRNVRLALAHATDKQ
                     KLIDVVLLGFGSPGLTLIPDGLGHWYNDSIQDYAFDVATANQILDDAGYADSDGDGVR
                     EMPDGSQPLNFRMHWPSDSIDAPRQAELLSEMWGEVGVSLELQAVDPDALTAECCPAF
                     DFDIILWGWGSDPDPNLLLSVHTTDEIPNGYNETGYSNPEFDELHIQQGRELDPEKRR
                     EIVWQMQQIAFDDVVYIIPYYQQAIQAYRVDRFTGWIEDQPKVELSDVSSLLVIEPVR
                     "
     gene            complement(2544..>2726)
                     /locus_tag="VFQ23_19290"
     CDS             complement(2544..>2726)
                     /locus_tag="VFQ23_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908803.1"
                     /translation="DISTLLEEQDEKELYESGFGHSVCTGAIFLRGNRCISTVDLAGG
                     VGGESGHFEPWYRSAL"
BASE COUNT          741 a          728 c          622 g          635 t
ORIGIN      
        1 tacgctgatc gcaatcaaca gaaatgctcc ctgcagcacc ggaaagtctc gcctgccgac
       61 cgattcatag atcgccagac ccagcccggg ccaggaaaat acggtttcga tttgaattgc
      121 tcctgccaca gtaaaaccca ggttgatggc aatgatcgtt acaattggca acatggcgtt
      181 cttcaaggca tggtccttga ggatctggaa gttgctcagc cctttcgctt ttgccgtcag
      241 aatataatct tctgcaagta catccagcag gctggatcgc atcagtagca aatactcgcc
      301 catatagacg atcgtgtagg tgaccgtggg caagagcata tgccgtgcga tatctcccac
      361 ttgaaacaat aacggataag agctgatccc cggtgtagcc ttcccgccta ttggaaatcc
      421 atggttgctt ccccagaaca ataggatgat cccaagccaa aacgtgggca aactccaggc
      481 aagcagaccg gtgatcaatg ctccatagtc gatcgaagtg cgtgccttcc acgctgcaaa
      541 tacaccaaag atcacaccta caaaaattga aagaatctgt ccggccccga tcaacaagat
      601 ggtattccaa agccgctcac caagaacgtc gaacaccgga cgattagtgt ggaaactgat
      661 tcccaactcg ccacgggtaa gattcctcaa atagataaaa aactgcgtat ccagtggatt
      721 gacgctacaa tctcccaact tgattgggtt aagcgtttca aaacaattga tgacgggttt
      781 gtccagtcca aatcgcacgc gcagggtttc gaccgcatcc ttcttcaagc gagggtcccg
      841 aataccggcg cgagcaggat ccccgggcag aacgcgaaag aggaaaaaat tcaggacgag
      901 aacaaagaga atcgtaaata gcgcccaacc aattttgttg aataagaaac gataacgcct
      961 caaagattgc tcccgggtct tccgtaacca aaattaaaga ggggcgacgc atctacagcc
     1021 gcccctcttt aatatcttat ttgcactacc tcacaggctc aatgaccagc aaagaactca
     1081 catccgacaa ttcaaccttg ggttgatcct caatccaacc ggtaaaccga tcgactcggt
     1141 acgcctgtat ggcctgttga taatacggga tgatatatac cacgtcatca aaggcgattt
     1201 gttgcatttg ccagacgatc tctcttcgtt tctcagggtc aagctctcgt ccctgttgaa
     1261 tatggagttc gtcaaattcg ggattcgagt atccggtttc attgtagccg ttcgggattt
     1321 catccgtcgt gtgaaccgag agcaataggt tcggatcggg atcagacccc cacccccaca
     1381 ggatgatgtc gaaatcaaag gcagggcagc attcggctgt cagtgcatcc gggtccactg
     1441 cctgcaattc caacgacaca ccaacttcac cccacatctc agagagaagc tctgcctggc
     1501 gcggcgcatc aatgctatca ctgggccagt gcatacggaa gtttaggggt tgtgaaccat
     1561 cgggcatctc acgaacgccg tcaccatcag agtccgcata ccctgcatcg tcgaggattt
     1621 gatttgcagt agccacgtca aatgcataat cctggatgga gtcgttgtac cagtggccta
     1681 gcccatcagg gatcaaggta agacccggag acccaaaacc caacaacaca acatcgatca
     1741 gcttttgttt atctgtcgca tgcgccagcg caagccgcac attccggtcg cgtagagccg
     1801 gatgacctgt gcacagcccg cctgcatcgg ttgggcaatt ctcagggtca gcctgattaa
     1861 aaatcacgtc tgaaacgtct ggagcgaaag gtgctccggt cacaactttg atgttggtat
     1921 caccctgcag tgtttcgacc gcggtagcag gcatttcagt aatcatatcc acctgaccac
     1981 ttcgtagaga ctgcactaag acatcgtcgc tctcaaaagt ttgaaagacc acctcgtcaa
     2041 tcttagggga agctgcaaaa tgctctttat tggcagtaag ccgtacaaac tcattctggc
     2101 tatattctgc catcttaaat ggaccggtac cgatcatttc cacgttttca aattcaacgg
     2161 atgcgggagc ttctgcattt gcactccaaa tgtgctctgg cacaacatat agataggaga
     2221 gcaggtaatc catatttggc acggcgtcgg tcagggtaat gacaaccgta ctgtcatccg
     2281 tagcttccac ggtatcaaag tttgcagtgt aggcattcaa gtaaatgtag tcttcattgt
     2341 ttttgtataa atttaccgag aatgctacat cattggcagt catcggctgg ccatcatgga
     2401 agttgattcc atcgcggatt ttaaatgtcc atgtcaatcc atcatccgaa acatcagcgc
     2461 tttcagcgac gtccaatttg taggtaccat cgagctgata ttcgtagatc gtgtcataca
     2521 ccaatgcaaa gatggtatag gcttcagaga gcactgcggt accagggttc aaagtgtccg
     2581 gactccccgc ccaccccacc cgcaagatcg acggttgaga tgcagcgatt cccccgcaag
     2641 aagatagcac cagtgcaaac actgtgacca aaaccagact catataactc tttttcatcc
     2701 tgctcctcca gcaaagttga aatgtc
//
LOCUS       DASTJP010000661         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_582640, whole genome shotgun sequence.
ACCESSION   DASTJP010000661 DASTJP010000000
VERSION     DASTJP010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582640"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2361
                     /locus_tag="VFQ23_19295"
     CDS             <1..2361
                     /locus_tag="VFQ23_19295"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9908804.1"
                     /translation="WLKLKRKALDLTREQLAERVGYSAATIRKIEDEERYPSAQVVER
                     LAGFFKIPQNELEDFLRFARGDLKSVPVEIDENVPWRASSKATRSNIPATTTSLIARE
                     KEIALVREYLSRDDIRLVTLMGPPGIGKTRLSIEVARASLHDFPDGVFFVSLALVDDP
                     DSISSTIIPALGFMESGRKAPEDQLKESIGEKQMLIVLDNCEHLVEFVASISSQLLSA
                     CPGLKLLTTSRESFRIPGEWLYPIPAFDIPTESEAIDLDNASDFPALMLFVERARAVR
                     PDFKLTADNIQTIAAICAHLDGLPLVIELIAARMRLMSPAALLERLSGQFVLTADGMR
                     APSERQKTLKNAIEWSYQLLSAQEQKLFVNLSVFSGGFTLKDAETIFARTVTEKSVPE
                     LLTLLLDKSLIRRVANETDEDQYAMLVTIQEYALERLRDSDEETKIRDWHLAYFLGIA
                     EKAAPELHRHNQLDWIDRLRTRRDNLRTALDWAIETERTESALQLVRNLDWFWHLRSD
                     YVEGAHWVLNVLKLPDVPLYPEAHAGVLVQLQHYKHLLGSHFSTQLLKGGGTSFAEQA
                     LSIARARNDANNSAGALAMIGLNLITEKNFAEAQSALEESRTLFQQSQDEWGYAYAVF
                     LLGWKSFVQNDLAAALTVIEQAFTIFEKLGERHFMCVALRYIGVIHIKQGNSKAGLEA
                     LKESLRLARQLDIKYEIAAAFYRLGEAAQYLGSSARTVSLFWAAKTAYDTINAGMWTQ
                     GMDAEFEPVLDRCRAELGEAAFAQAVEQGRAMTMEQAVAYALENSS"
     gene            2573..>2791
                     /locus_tag="VFQ23_19300"
     CDS             2573..>2791
                     /locus_tag="VFQ23_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908805.1"
                     /translation="MNKKISRRDALKVGALGVLGGLAACLPVARATETLIPTYAPFPT
                     GTPVPSVATVPGITDVAPTAGPVLDTLIN"
BASE COUNT          664 a          698 c          738 g          691 t
ORIGIN      
        1 tggcttaagc tcaaacggaa agccctcgat ctcacccgcg agcaattggc ggaacgcgtg
       61 gggtactccg cggcaaccat ccgtaagatt gaagatgagg aacggtatcc atctgctcag
      121 gttgttgaac gcctggcagg attctttaag attccccaga acgagctgga agacttttta
      181 cgtttcgcac gcggcgactt gaaatctgtg cccgttgaga tcgatgaaaa cgtgccttgg
      241 cgcgcttcgt ccaaagctac tcgttcgaac atccccgcta ccaccacctc gttgatcgcc
      301 cgtgaaaaag aaattgccct tgttcgcgaa tatctatcga gggatgatat tcgtctcgtt
      361 acgttgatgg gcccaccggg aattggcaag acgcggctaa gcatagaagt ggcgcgcgcc
      421 tccttgcatg actttcctga tggagttttc tttgtctcac tggctttggt ggatgaccct
      481 gattcgatct cctcaacaat cattccagcg cttggtttca tggaaagcgg ccgcaaggca
      541 cccgaagacc aactcaaaga aagcattggt gagaaacaga tgctgattgt cctggataat
      601 tgtgagcatc tggttgaatt tgtggcttcc atctcctcgc aacttctctc cgcctgtccc
      661 ggtttgaagc tattgaccac cagccgggag tcgtttcgca ttcctggcga atggctgtat
      721 cccatccctg catttgatat ccccacagaa agcgaggcga ttgaccttga caatgcctca
      781 gatttccctg cgttgatgtt atttgtcgaa cgcgcccgcg ctgtgagacc cgatttcaaa
      841 ttgactgctg acaatatcca gacgattgcc gcgatctgcg cgcatctgga tggcttgccg
      901 ctggtgatcg agttaattgc cgcacggatg cgcctgatgt cgccagcggc attgctggag
      961 cgtttgagcg gtcaattcgt tttgactgcc gatggcatgc gcgcgccctc tgaacgccag
     1021 aagacgttga agaacgccat tgagtggagt tatcaactgc tctctgccca ggagcaaaaa
     1081 ctgttcgtga acctctctgt gttttcaggc ggcttcacgc tgaaggatgc cgaaacaatt
     1141 tttgcgcgca cagttactga gaaatctgtg ccagaactgc tcacgttact tctggataag
     1201 agcctgatac ggcgggtggc aaatgaaaca gatgaagacc agtatgcgat gctggtgacg
     1261 attcaggaat atgctcttga gcgtttaaga gattccgatg aggaaaccaa gatccgggac
     1321 tggcacctgg cttattttct gggtatagca gagaaagccg ctcctgagtt gcatcggcac
     1381 aatcaactcg attggatcga tcgcctgcgc accaggcgcg ataatctgcg caccgctctg
     1441 gattgggcga ttgaaacaga acgaaccgaa tcagccttgc agctagttcg caacctcgac
     1501 tggttctggc atcttcgttc agactatgtg gagggagcac attgggtgct aaacgttttg
     1561 aaactgccgg atgtaccttt atacccggag gcgcacgccg gggttctcgt acaactgcag
     1621 cattataagc atttgcttgg gagtcacttc tccactcaac ttctgaaagg cggaggaact
     1681 tcgtttgccg aacaagccct ctcgattgcg cgtgcacgta atgacgcgaa taacagtgct
     1741 ggcgccctgg cgatgattgg tctgaatttg atcacagaaa aaaactttgc agaggcgcaa
     1801 tcggcgctgg aggaaagcag gacgctgttt cagcaatccc aggatgaatg gggatatgca
     1861 tatgctgtgt ttctactcgg ctggaaatcc tttgtgcaaa atgatctggc ggcggcgctc
     1921 acggtcattg aacaggcatt tacgatattc gagaaacttg gcgaaagaca ttttatgtgt
     1981 gtagctttaa gatatatcgg tgtcatccac ataaaacaag gtaactcaaa agctggactt
     2041 gaagccttga aagaatcctt gcgcctcgca cgccaacttg atatcaaata tgaaatcgcg
     2101 gctgcgttct atcgtttggg agaggctgcg cagtacctgg gaagttcggc ccggactgta
     2161 tctttattct gggcagcaaa gactgcctac gatacgatca atgcaggtat gtggactcaa
     2221 ggcatggatg ccgaatttga acctgtcctt gaccgttgcc gcgctgagct tggcgaggcg
     2281 gcatttgcac aggcagtaga acagggtcgc gccatgacca tggaacaggc agtcgcgtac
     2341 gcgcttgaaa atagttcctg atttcgaatc agcgtgaatt atcgagcgcc gcgcatgtct
     2401 gacatgcgtg gcgaagccaa aaatcacttt tttctcccaa attgtgttaa aagattaagc
     2461 aatttctaag cgggctgcaa ggtagggctg atataaaaat ccagatagat tgatcgtacg
     2521 tatctgtttc aatcagtata tgttccgtag tgtaccgttt ggaggtatcc ccatgaataa
     2581 aaaaatctcc cgccgcgacg ccttaaaagt aggcgcacta ggagtgcttg gcgggttggc
     2641 tgcctgctta cccgttgccc gcgcgaccga gacgttaata cccacgtatg ccccgttccc
     2701 aactggtacg cctgttccat ctgttgccac tgtgcccggc ataacagacg tagcgcctac
     2761 ggcggggccg gtcctggaca cattgatcaa t
//
LOCUS       DASTJP010000662        14177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_181962, whole genome shotgun sequence.
ACCESSION   DASTJP010000662 DASTJP010000000
VERSION     DASTJP010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181962"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..683)
                     /gene="merB"
                     /locus_tag="VFQ23_19305"
     CDS             complement(174..683)
                     /gene="merB"
                     /locus_tag="VFQ23_19305"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HET9908806.1"
                     /translation="MDNNESILWQVRHFVYHHLADTTRAPTVKETALHFDISNEEASE
                     LYQELHNRHAFFLEPQTLTIRMANPFAGIPTDFKVHANGKTYYANCAWDMLGIPAALH
                     TDAIIEAVCTESNESVKLEIKNGHVILSDSEGSLPSTSETLRSAQSDTLLIHFPLPFV
                     RWYDDLVFT"
     gene            complement(691..1452)
                     /locus_tag="VFQ23_19310"
     CDS             complement(691..1452)
                     /locus_tag="VFQ23_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9908807.1"
                     /translation="MRTSIEWRKYFEENAQSLLKIPWHLGHELSEEEQKAITSSVQGF
                     QAGESSEGRHLFHYAKNYADQSGDYEYVEAIRLFIGEEQRHARDLAHFLQINGIPLVK
                     RTFTDGVFRRLRQILGNLEVSISVLITAEIIAKVYYTALRDATHSSVLQTLCRQILQD
                     EVQHVEFQAQRLGILRAKRNRVWFVMTNGLQSFLFCGTCLVVWIFHRKVFIKSGYPFV
                     EFWKYCWKEFIKALKISRNAMIIMSMNNELVLVEP"
     gene            complement(1577..1993)
                     /locus_tag="VFQ23_19315"
     CDS             complement(1577..1993)
                     /locus_tag="VFQ23_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908808.1"
                     /translation="MNKQNILSSHTIDNNAALKLFGLPRALAGALIGVLIFLLIFLPL
                     KFLDYNSVLLYTLALDLELPGRLTILILGMTAGIDLPKALLNVLSITISAIPAGIAGR
                     LISSLNKSTRTTGIVFSLIYLCFILAFGALLTFLGI"
     gene            complement(2014..2457)
                     /locus_tag="VFQ23_19320"
     CDS             complement(2014..2457)
                     /locus_tag="VFQ23_19320"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9908809.1"
                     /translation="MTFQNDPDVIRWKLHLKSSPQVVYQKLSTDEGRASFWAESAMEE
                     NGVIHFVFPNDAEWKGKILDKEPPHQFKVEYYGGSITTFELHPDDVGGTDLTLADQGV
                     HPEDRTEVIAGWISVLMALKAYVDFGVDLRTHDRSRTWDEGYAEN"
     gene            complement(2454..3728)
                     /locus_tag="VFQ23_19325"
     CDS             complement(2454..3728)
                     /locus_tag="VFQ23_19325"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET9908810.1"
                     /translation="MTNSRISGFYNMTLDERRAQLANVADLSAETLLPFTAGGLSPDA
                     ADHMIENVIGLYSLPLGIALNFQVNGRDVLVPMTLEEPSVVAGASFMAKLARAGGGFT
                     ATTTDPLMIGQMQVINVHNPHEAKLKLYEHKADLIAEADTIDPILKKFGGGARDLEVR
                     IIESSPIGPFLVVHLIYDVRDAMGANAVNTACERLAPRVEGITGGKVHLRILSNLADR
                     RVARARCTIPLDELAFNNFSGEEVRDGIIAAYAFAAADPYRAATHNKGIMNGVDSVVI
                     ATGNDWRAIEAGAHAYAARSGRYTSLSTWDKDINGNLVGTLEMPMAVGIIGGATRVHP
                     AAQASVKLMGVKTAGELAEIIVSVGLAQNMAALRALATEGIQRGHMSLHARQVAIAAG
                     ATGELIEKVAGQMVVEKVVRIDRAEEILKGMQ"
     gene            4400..5779
                     /gene="dnaA"
                     /locus_tag="VFQ23_19330"
     CDS             4400..5779
                     /gene="dnaA"
                     /locus_tag="VFQ23_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9908811.1"
                     /translation="MNAEQAWQSVLGQLQMEMPRASFDTWVRDTKPVSYHNGTLTISV
                     RNAYARDWLESRLASTISRLLLTIMNVSVAVNFIVSNNEPDQTIDPRENSLTFPQDAN
                     TTHTQEQIDAYDQPSRSSGLNPRYIFDTYVVGSGNRLAHAACLAVAEKPARAYNPLFL
                     YGGVGLGKTHLLHAIGNACHQRGLNVLYVSSEEFTNDMITAIRTHTNQAFREKYRSAD
                     VLLVDDIQFIAGKESTQEEFFHTFNTLHGQDKQIIVSSDRPPKSLVTLDERLRSRFEW
                     GLTADVQLPDFETRLAILRSKAERTGRQISDDILDSIARRVQSNIRELEGALNRILAF
                     ADLSGTSLTPHLVEVALADLLPQRTDIPPQKIVELVAKEWQTTVEALLGRDRSQKIVQ
                     PRQVAMYLLRKETDASLPQIGEVLGGRDHTTVMHSIDKITSDIETKADLRKRVVSVKQ
                     QLYGQGAVI"
     gene            6062..7018
                     /gene="rpsB"
                     /locus_tag="VFQ23_19335"
     CDS             6062..7018
                     /gene="rpsB"
                     /locus_tag="VFQ23_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET9908812.1"
                     /translation="MAVISMKALLESGVHFGHRTNKWDPRMKPYIFTERNGIHIIDLQ
                     QTSKLLNQAYNVIRDAVTNGGTVLFVGTKRQAQETVAEEATRCGMPYVTERWMGGMLT
                     NWSTMHARIQELERLEKRRDNNELERLTKKEGLLIGREITRLTTRLSGTRTMKRRPDV
                     LFIIDVGREEAAVHEANLLGIPIVAMVDTNCNPLNVDYVGPSNDDAIRAIKLLVAKIA
                     DAVLEGKAMRKEEDLEEKPAESTKSKTDRSARKSPKIVETDEELEDKALLGESTLAKM
                     SAAREDVVVETPAAPATPPVVEEPAPTPAAPETPATEEKSAE"
     gene            7025..7648
                     /gene="tsf"
                     /locus_tag="VFQ23_19340"
     CDS             7025..7648
                     /gene="tsf"
                     /locus_tag="VFQ23_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET9908813.1"
                     /translation="MTRTIEMEITTDMIKELRAATNAGVLDCRKALTEANGDFQKAVD
                     WLREKGMATAAKRADREASQGVIELYSHGGGRVGVMVEVNCETDFVARSDKFRELAHE
                     LALQIAASAPRYLKVEDIPADVLEHEAEIARARAKEEGKPENVTEKIVQGRLEKFKDE
                     VVLHRQAYIRDESLTVEKLILQTVAAIGENVIVRRFQRWELGESLNN"
     gene            7786..8499
                     /gene="pyrH"
                     /locus_tag="VFQ23_19345"
     CDS             7786..8499
                     /gene="pyrH"
                     /locus_tag="VFQ23_19345"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET9908814.1"
                     /translation="MSELKYKRVLLKLSGEALASQSGFGIDVDEAESIASRIQEVYNM
                     GVEVAVVIGAGNLWRGKQGLERGMDRSTADYMGMLGTVMNAMVLMDALERIGVYTRVM
                     SAIEMRAVAEPYIRRRAIRHLEKGRVVIFGAGTGNPFFSTDTAAALRATEIDADVVIK
                     ATKVDGVYDSDPKKNPDAKKFDELDYIEVINRRLGVMDSTAITLCMENKLPILVVNLW
                     DPHALLNALKGEAVGTLVH"
     gene            8716..9273
                     /gene="frr"
                     /locus_tag="VFQ23_19350"
     CDS             8716..9273
                     /gene="frr"
                     /locus_tag="VFQ23_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET9908815.1"
                     /translation="MIKDILTDAEHRMRSAIQVLHDDLAAIRTGRASPSLVEKLSIEY
                     YGSPTPLMQLASISVPEPRTLTIKPFDASTLKVIEKAIMTSELGLNPNNDGKVIHLNL
                     PPLNEERRRDLVKHVHHRLEEARIAVRNIRRDSHNDMRDFEKEKLITEDDLERGEEDL
                     QKLTDRFIEEVAQHGKNKEIEIMEV"
     gene            9284..10030
                     /locus_tag="VFQ23_19355"
     CDS             9284..10030
                     /locus_tag="VFQ23_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET9908816.1"
                     /translation="MASSSDNLPVDKVPRHVAIIMDGNGRWALSRGLPRLAGHKAGTE
                     NLRRVIRATVEFGVKYLTIYAFSTENWGRPPEEVKGLMYILEDVIDRELAELSKEGVQ
                     LHHIGRLERLAPSLQKKVLHAIELTKNNDRLILNVAFNYGGRDEIVNAIQNIMKDGIP
                     PEDVTDALVSSYLYTAGVPDPDLIIRTSGELRISNFLIWQAAYSEWYVTPTYWPDFGK
                     DEYRRALETFVQRDRRYGKVKSKEYEATNA"
     gene            10023..10841
                     /locus_tag="VFQ23_19360"
     CDS             10023..10841
                     /locus_tag="VFQ23_19360"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9908817.1"
                     /translation="MLRRTLTALVLVAIGLPGIIYGGVYYFVLITIFLVGSAWEYVRL
                     YRAVQYEPHDILTIGGVLAIATARVFFVEFTLVLFALLILLAMTVHLVAFERGRDQAA
                     LDFCVTVAGIVYLGWLGSYLLDLRNLDLGVWWLMLVLPIVWGGDTGAYSIGAAYGKHK
                     MTPRLSPKKSWEGYFAGVFTSMIIGAFFAYAFSALGPQPLNGLIDPLRGGLLGLVIGA
                     VVPLGDLGESMIKRQSGLKDSSNIFPGHGGFLDRIDSWIWGAVIGYFAIQLFIL"
     gene            10852..11841
                     /gene="glpX"
                     /locus_tag="VFQ23_19365"
     CDS             10852..11841
                     /gene="glpX"
                     /locus_tag="VFQ23_19365"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET9908818.1"
                     /translation="MEFGSDPSRNLALELARVTEAAALAAGRFMGRGDKEAADQAAVN
                     AMRIVLQTVDMNGVIIIGEGEKDNAPMLYNGENVGNGSAPDVDVAVDPIDGTRPLAFG
                     RSNSLATVAIAPRGTMFDPGPFVYMNKLAVGPEAKGKIDIERSITDNLKAIAKAKRKD
                     VEDLTTIILDRDRHKDMVAEIRKAGARIRQIPDGDVAAALMTAWPDSGVDVLIGVGGT
                     PEGVIAACALSAMGGEIQGKMYARDEDELRRGREAGYDFDKVLTMNDLVASEEVFFAA
                     TGITDGELLQGVRYFGDGASTDSLVVRGLTGTVRQITATHRIDKLRHLSAVKY"
     gene            11926..12216
                     /locus_tag="VFQ23_19370"
     CDS             11926..12216
                     /locus_tag="VFQ23_19370"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9908819.1"
                     /translation="MLLQENTSTHLADLFSALSDPTRLRIISVLLDSEMNVGEIATQL
                     EMTESAVSHQLRGLRHMRLVRARKNGRQVYYALDDDHVAKLYRLGLEHVDHG"
     gene            12220..13194
                     /locus_tag="VFQ23_19375"
     CDS             12220..13194
                     /locus_tag="VFQ23_19375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9908820.1"
                     /translation="MHTHSHSHSHFGDIAKQTTSRMAISLTLTLVFVFIEAFAGIVGN
                     SLALLTDAAHNLTDVIALGLSWFAIRLTTQPSNAQKTYGYHRAGILVALINSTTLVVI
                     SLGIFYEAWRRFSNPPEVQSGILITVGLIAVVINLVTALLVRKGSEHDLNIRSAFLHL
                     MGDVVSTVGAVIAGVIIYFTGANWLDPLVSVLIGLLILYNAWGILKDAIDILLEATPR
                     DVDMQAMVKDIAKVDGVIGVHDIHVWSLTQSLRTMSAHILTDDLPISAGAMIQNQINE
                     ILFHRYKIAHATLQLECVECSPDSLYCDLNGHVHVGDVSTLHRDDGHS"
     gene            13311..13805
                     /locus_tag="VFQ23_19380"
     CDS             13311..13805
                     /locus_tag="VFQ23_19380"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9908821.1"
                     /translation="MKYLLKIWRVLPLWLQIILSRFIRPLFQVFAAGVIFNEDDEILL
                     VKSTYQRFHPWGLPGGSLEYGESAEEAVKREVWEETGLVIEIKRLLLVKTWSPDRVGM
                     YYLCEVTGGGIHPTDEVSEAGYFALDDLPDVRSEDIGLIKELHRMTDLQSLTMEKVLL
                     ETKL"
     gene            13824..>14177
                     /locus_tag="VFQ23_19385"
     CDS             13824..>14177
                     /locus_tag="VFQ23_19385"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET9908822.1"
                     /translation="MNWLEVSLTVNGELAEAVADVLARFAYSGVMMEQGVKYTDDEDA
                     GTPTGPITVRAYLEVNDNLEETRQKLEESLYYLGLIQPLPAASYKEIADQNWMEAWKQ
                     HYKPILIGKRLVIVPA"
BASE COUNT         3528 a         3376 c         3633 g         3640 t
ORIGIN      
        1 tctgcaactt gctcgaagct tcgcccatga taatccggtg acaggcgatt gccataccac
       61 aactttgaaa gatcccaaac ttgacgaaga gttagtgttt ctcctcgttc caggttattt
      121 cgtttgcacc atttgtcgat ccattcctcc gaccggaaga gcaacatcgt ggctcaagtg
      181 aagaccaaat catcgtacca gcggacgaag ggcaacggga aatgaattag taatgtgtca
      241 ctctgagcgg agcgaagagt ctcgcttgtg gatggaagag atccctcgct atcactcagg
      301 atcacatgtc cgttttttat ttccagtttt accgactcat tactctctgt acaaacagct
      361 tcgatgatcg cgtctgtgtg tagcgcggca gggatgccga gcatatccca ggcgcagttg
      421 gcgtaatagg tcttaccgtt ggcatggact ttgaaatctg tggggattcc tgcgaacggg
      481 tttgccatgc ggatagtcag cgtctgtggc tcgaggaaga atgcatggcg gttatgtaat
      541 tcctggtaaa gttcgctcgc ttcttcatta ctaatgtcga agtgaagcgc tgtctccttc
      601 acggtgggag cgcgagttgt atccgccaaa tggtggtaga cgaagtgtct gacttgccag
      661 aggattgatt cgttattatc cattatcaat ttagggctca actaaaacca actcgttgtt
      721 catgctcatt attatcatag cgtttcttga aattttaagg gctttgataa attctttcca
      781 gcagtatttc cagaattcca caaatggata accacttttt ataaatacct ttctgtgaaa
      841 gatccacaca accaggcatg ttccgcagaa taaaaaactt tgtaagccgt ttgtcatgac
      901 aaaccaaacc cggtttcgct ttgctcgcaa gattccgagt ctttgtgcct gaaattccac
      961 atgttgcact tcatcttgca ggatttgcct gcacaaggtt tgaagaacac tggaatgcgt
     1021 cgcgtctctt aatgcagtgt aatagacttt tgcgatgatt tccgcagtga ttaaaaccga
     1081 aatggaaact tcaagatttc ccagtatttg acgaagacga cggaatacac catctgtgaa
     1141 agttcttttt acaagcggaa tcccgttaat ttgcagaaaa tgagctagat ctcgggcatg
     1201 ccgctgctcc tcgccaataa atagacggat tgcttcaaca tattcatagt cacctgattg
     1261 gtcagcataa ttctttgcat aatgaaacaa gtgacgaccc tccgaacttt cgcctgcctg
     1321 aaagccctga acggaagaag tgattgcttt ttgttcctcc tctgaaagtt catgccccaa
     1381 atgccagggt attttgagaa gcgattgggc gttttcttca aagtattttc gccattcgat
     1441 ggatgttcgc atgatgatct ccaatcttta agagtagtga ccatcattct agttaatatg
     1501 ctctttcaaa gcctctctca gtctatcaaa ttcaatccta tcttttgatc ttctctaaaa
     1561 aggagaggtg tttcttttag attcccagaa acgttaataa cgctccgaag gctaaaataa
     1621 aacaaagata tatcagagaa aaaacgatcc ctgtcgttct tgtcgattta ttgagcgagc
     1681 taatcagcct gcctgcgatt ccagcgggaa tggcggagat tgtgatgctc agcacattca
     1741 gcagagcttt aggcagatcg atgcctgccg tcatgccaag tataaggatg gtcaacctac
     1801 cgggcaattc caggtccagc gcgagtgtat ataggagcac cgagttgtaa tcaagaaatt
     1861 tgagaggcag gaaaataaga aggaagatca gaacgccgat caacgcaccc gcgagagctc
     1921 tcggcaagcc aaacagcttt aaggcggcat tgttatcgat ggtatggctg ctcaggatat
     1981 tttgtttgtt cattacctgt tcacgtagcc tggttagttt tcagcatacc cttcatccca
     2041 ggtgcggctg cggtcatgtg tgcgaaggtc caccccaaaa tccacgtagg ctttgagcgc
     2101 cattaatacg gaaatccagc ctgcgatcac ttccgttcga tcctccggat gcacaccttg
     2161 atcagcgaga gttaagtccg tgccgcctac gtcatccgga tgtagctcaa aggtagtgat
     2221 gctgcctcca taatattcca ctttgaattg atgaggtggc tccttatcta gtatttttcc
     2281 tttccactca gcatcattgg ggaagacaaa atgaatgaca ccattctcct ccatcgccga
     2341 ttctgcccag aaactggcac gcccttcatc agtagagagt ttttggtaaa cgacctgtgg
     2401 agacgacttc aaatgtaatt tccaacggat gacgtccggg tcgttctgaa aggtcattgc
     2461 atccctttca aaatctcttc ggcacggtcg attctcacta ctttctcgac aaccatctgc
     2521 ccggctacct tttcgatcag ctcacctgtg gctcccgctg cgatagcaac ctgccgcgca
     2581 tgcagggaca tgtgtccgcg ctggatgcct tcagtggcga gcgctcgtaa ggccgccatg
     2641 ttttgcgcta accccactga gacgatgatc tcagccagtt ctcctgctgt tttcactccc
     2701 atgagtttga cagaagcctg cgcggccggg tgtacgcggg tcgcgccacc gatgattccc
     2761 accgccatcg gcatttcaag tgtccctaca aggttgccat tgatatcctt atcccatgta
     2821 gataaagaag tgtacctgcc tgaacgtgct gcataggcgt gtgcaccagc ctcaatggcg
     2881 cgccagtcat tgcctgtggc gattacgacc gaatcaacac cgttcatgat gcctttgtta
     2941 tgtgttgccg ctcgatatgg atctgccgcg gcgaaggcat aggcagcgat aatcccgtca
     3001 cgcacttctt cgcctgaaaa gttattgaaa gctaattcat ccaacggaat ggtgcagcgc
     3061 gctcgcgcaa cacgccggtc ggcgaggttg gaaagaatgc gcagatgaac ttttccgccg
     3121 gttatccctt caacgcgagg agcaagccgt tcacaggcgg tatttacggc gtttgcaccc
     3181 attgcatcgc gtacatcgta gatgagatgg acaaccagga agggtccgat cggggaggat
     3241 tcaatgattc gaacttcaag gtctcgtgca ccaccaccaa atttcttgag gatcgggtca
     3301 atcgtatctg cttcagcaat taagtcagct ttatgttcat acaacttaag cttggcttca
     3361 tgcggattat ggacattgat gacttgcatt tgaccgatca tcaacggatc tgttgtggtg
     3421 gccgtgaatc caccaccggc gcgggcaagt tttgccataa aggaagcgcc cgcaaccaca
     3481 gagggttcct ccaacgtcat tggcacgagc acatcacgtc cgtttacttg aaaattcaac
     3541 gcaattccca ggggaaggga atataagccg atcacatttt cgatcatatg gtcggccgcg
     3601 tcaggcgaga gtccacctgc agtaaacggg agcagagttt cggcagaaag atctgcaacg
     3661 tttgccagtt gagcacgtcg ttcatcgaga gtcatgttgt aaaagccgga gatgcgagag
     3721 tttgtcatgg tttcaccttt aatgaataca ttgtattctc atcctctttc aaaagctatc
     3781 atattttata taaacgaata ccgttattca atggggatgt gtacgtgatc cgatttgttt
     3841 cagaacttat cgctacaata cttacattgc tttatttaac gcgcgtacaa aaacaatatg
     3901 ttgagattta ctttgaaatt gaatcccagg attcggaggt tcaaacttaa agaaaaatga
     3961 atgggtctct tggcattttt taaacaaacc acctccaaat atgtattcta agtgtcaatt
     4021 ttatgatgta acaccatatg tggggggtat caaaaaatca ttcagtgtac gtggctgaaa
     4081 cacgtgttct ataaaatttt cgattccacc aacattcacg cgatgaatct ttaacgttgg
     4141 atgaccttaa aaaaacgaat gtttttaaac tagcgtaggc tttgcgtgtt gcatttttat
     4201 ttttatgtgc tagaatgtgg cgacgttgag agaagatgtg gtgagtaata ctcatcttga
     4261 atccctgtag ttcgtgaatt acaactaaat atacttcaaa caggccgctg atgaggcggg
     4321 ttagttcgac gtgtgcaatt atcccgctct tatccagagc gcataagcgg gcgggattat
     4381 cctcggtatg gagcaaagta tgaatgcaga acaagcgtgg caatcagtgc tgggtcaact
     4441 tcaaatggaa atgcctcgtg cgtccttcga tacatgggtg cgcgatacca aacctgtttc
     4501 ctaccataat ggaacgttga ccatcagtgt gcgcaatgca tacgcacgcg attggcttga
     4561 aagccgcctc gccagcacca ttagccgcct gcttctgact atcatgaacg tcagcgttgc
     4621 tgtcaatttt atcgttagta acaacgaacc tgatcaaacc atcgatccca gagaaaattc
     4681 cttaaccttt ccacaggatg caaatacaac tcatacacag gagcagatag acgcctacga
     4741 ccagccgagc cgcagctctg ggctgaatcc acgttatatc tttgacacat atgttgtggg
     4801 gtcgggaaat cgtctcgctc atgcggcctg ccttgcggta gctgagaagc cggcgcgcgc
     4861 ctacaatccg ctatttctgt atggaggggt gggtctcggg aaaacccacc tcctccatgc
     4921 cattgggaat gcctgtcatc agaggggact taatgtttta tatgtatctt ctgaagaatt
     4981 cacgaatgat atgatcactg ccatccgcac gcacacgaat caggcgtttc gtgaaaagta
     5041 tcgctctgcc gatgtattgc tggtagacga tatccaattc atcgctggca aggaatctac
     5101 ccaggaggaa tttttccaca cctttaacac actgcatggt caggataaac agatcatcgt
     5161 ttcatcggac cgccccccga aatcccttgt aacgctggat gaacggctgc gctctcgctt
     5221 tgaatggggc ctgactgccg acgtccaact gccagatttt gaaactcgcc tggccatttt
     5281 gcgctcgaaa gccgagcgaa ccggtcgtca gatttccgat gatatattgg atagcattgc
     5341 acggcgcgtg cagtccaata tccgtgagtt ggaaggcgct ttgaatcgaa tccttgcctt
     5401 tgcagatttg agtggcacct cactaacccc gcatctcgtc gaagtggctc ttgcagactt
     5461 attacctcag cgaacggata tcccacctca aaagattgtc gagctggtgg ccaaagaatg
     5521 gcagaccact gtggaagccc ttctcggtcg cgaccgctct caaaagatcg tccaaccgag
     5581 acaggtggcc atgtatctgc ttcgaaaaga gacggatgcg tctctgccgc agatcggcga
     5641 agtgcttgga ggccgcgatc acacaacagt gatgcactcc attgataaaa tcaccagtga
     5701 tattgagacc aaagcagatt tgcgaaagcg cgtggttagt gtcaagcagc agttatacgg
     5761 tcaaggtgca gtaatctgaa gcatcggtcc cgagcttgct cggggctttt tttatccacc
     5821 atgccgaagc aatatttgcc ttttataaga gattcttgtg atataatccc gccgcttccc
     5881 tgttcgactt cggcacaaca atcacgtacc ttcgctcagg gtgaagcaca tcttgcctgg
     5941 gacgggcatc aagcgtccct gagcgggtct ggctttcgag ccgtatcccc gctaaactca
     6001 ttccactccg cgccggccga agcgttcgcg cgttggagag tggaacagta aggagaaata
     6061 catggctgta atttctatga aggccctgct tgagagcggt gttcattttg ggcatcgcac
     6121 caacaaatgg gacccgcgca tgaagccgta tatttttacg gagcgcaacg gcattcacat
     6181 tattgacctg cagcagactt ccaagctgtt gaatcaggcc tataacgtga tccgtgacgc
     6241 agtaacgaat gggggcaccg ttttgtttgt cggtaccaaa cgccaggcac aggaaaccgt
     6301 tgccgaagag gcaacgcgct gcggtatgcc atatgttacc gaacgctgga tgggcggtat
     6361 gctcaccaac tggtccacca tgcatgcccg cattcaagaa ctcgaacggc ttgagaagcg
     6421 ccgtgataac aacgaattgg aacgcctgac caagaaggaa ggcttgttga ttggtcgcga
     6481 gatcacgcgc ctgaccacac gtctctctgg aacgcgcacc atgaagcgcc gacctgacgt
     6541 actcttcatt atcgatgttg ggcgtgaaga ggccgcggtg catgaagcaa acctgcttgg
     6601 cattccgatt gttgcaatgg tggataccaa ctgcaaccct ttgaatgttg attatgtcgg
     6661 tccttcaaac gatgatgcga ttcgtgcaat taagttgctt gtggcaaaga tcgctgacgc
     6721 ggttctggaa ggcaaagcga tgcgcaagga agaagacctc gaggagaagc cggcggagtc
     6781 gaccaaatcc aaaacggaca ggtctgcacg aaagtctcca aaaattgtcg agaccgatga
     6841 agagttggaa gataaggctt tgctgggcga atccacgtta gccaagatga gcgcggcccg
     6901 cgaagacgtg gttgtagaaa cgcctgcagc gcccgctacg cctcctgttg ttgaagaacc
     6961 ggcgcccaca cctgctgcgc ctgaaacacc tgctaccgaa gaaaaaagtg ctgaataagg
     7021 caaaatgaca aggactattg agatggaaat tacaacggat atgatcaaag aactacgcgc
     7081 cgcaacgaat gcgggcgttt tggattgccg aaaagctctt acggaagcaa atggtgactt
     7141 tcaaaaagca gtcgactggc tgcgagaaaa aggaatggca acggctgcaa aacgtgcaga
     7201 tcgggaagcc tcacagggcg tcattgaatt gtactcccat ggcggcggac gagttggtgt
     7261 catggtcgaa gtgaactgtg agaccgattt tgttgcccgc tctgataaat tccgtgaact
     7321 ggcacatgag cttgcgttgc agatcgctgc gtcggcaccg cgttatctta aagtggaaga
     7381 tatcccggct gatgttctgg aacacgaagc ggaaattgcc cgtgcccgag cgaaagaaga
     7441 aggcaaaccc gaaaacgtca ccgaaaagat cgtacaagga cgcctcgaaa agtttaagga
     7501 tgaagtggtt ctgcatcgtc aggcctatat ccgagatgaa tcgctcacag tggaaaaact
     7561 catccttcaa acggtggctg ccatcggcga aaatgtgatc gtgcgccgct tccagcgctg
     7621 ggagctgggc gagagtttga acaattaatc gaaaagacca accttcgggt tggtcttttt
     7681 atgaccccac ccgtcctcac ctgcacttgc attaggtgca agtgtcggac cacctcccaa
     7741 aattcgaaga atttgtggag gattggagag gggcgaagga gaaatatgag tgaactgaaa
     7801 tataaacgtg tccttctaaa actaagcggg gaagcgcttg ccagccaatc tggctttggc
     7861 atcgatgtag atgaagccga atccatcgcc agcagaatcc aggaagtcta taatatgggc
     7921 gtggaagtgg cggttgtcat tggggccgga aatttatggc gcgggaaaca aggactggaa
     7981 cggggcatgg atcgctctac ggcggattac atgggcatgt tggggactgt gatgaacgcc
     8041 atggtgctta tggacgcact tgaacggatt ggtgtatata cgcgtgtgat gtctgcgatt
     8101 gaaatgcgcg ctgtcgctga accgtatatt cgacgccgcg ccatccgtca cctggagaag
     8161 ggacgcgtgg ttatctttgg cgccgggacc ggtaacccgt tcttttcaac ggataccgct
     8221 gccgccttgc gcgcgacgga gatcgatgcc gacgttgtaa tcaaagccac caaggtagat
     8281 ggcgtctatg actcggaccc aaagaagaat ccggatgcca agaaatttga tgagctggac
     8341 tatatcgaag tcataaatcg tcggctcgga gtgatggata gcacggcgat tacgctttgt
     8401 atggaaaaca agttgcccat actagttgtc aatctctggg atccccatgc tctgcttaat
     8461 gctttgaaag gagaagcggt cggaacctta gtgcattagg ctcggttcaa accacgaaat
     8521 aggagagcgg agtctctcct gtttttattg tagagtctct cacttttttt caatttctct
     8581 cacgaattga cctttctgta gcgtttttca tggttttggt gtatccttat atagacttcg
     8641 aaagtctttt tcggagtcgt aaacgatgga attccaggga agacttccga gtctttctaa
     8701 tttcaggaga ttgtggtgat aaaagatatc ctaaccgacg ccgaacatcg gatgcgcagc
     8761 gcaatacaag tcttgcatga tgatctggcc gccatacgga caggacgtgc cagcccaagt
     8821 cttgttgaaa aattatcgat tgagtattac ggaagcccca cgcctctcat gcagctggct
     8881 tcgatcagtg tcccggaacc gcgcacgctg acaattaagc ccttcgatgc ttccaccttg
     8941 aaggtaatcg aaaaagcaat tatgacatca gaactgggct tgaatccaaa taatgacggg
     9001 aaagtgattc acctcaacct gccgccactt aatgaagaac gccgccgcga cctggtgaag
     9061 catgtccatc atcgcctcga ggaagcgcgt atcgccgtcc gcaatattcg acgcgactcg
     9121 cataacgaca tgcgggactt cgaaaaggaa aagctgatca cggaagacga tctggaacgc
     9181 ggcgaagaag atttgcaaaa actgacggat cgctttattg aagaagttgc ccagcacgga
     9241 aaaaataaag aaatagaaat catggaagtt taggtcaatt tcaatggcca gctcctctga
     9301 taacctcccg gtggacaaag ttccccgtca tgttgccatc attatggacg gcaatgggcg
     9361 atgggcgttg tcgcgtggtt tgccgcgcct ggccggtcat aaggccggga ctgagaatct
     9421 gcgccgcgtc atacgagcga cagtagaatt tggcgttaaa tacctcacca tctacgcatt
     9481 ttctaccgag aattgggggc ggcctccgga ggaagtcaag gggttgatgt atatccttga
     9541 agatgtgatt gatcgcgaac tggcggagtt gagcaaggag ggcgtacaac tgcatcatat
     9601 tgggcggctg gagcgcctgg ctccttcgct tcagaaaaag gttttgcatg ccattgaatt
     9661 gacaaagaac aacgatcgtt tgattctgaa tgtggcattt aattatggcg gccgtgatga
     9721 aatcgtcaac gccattcaaa atatcatgaa ggatgggatc cctcctgaag atgtgacgga
     9781 tgcccttgtg agcagctact tgtatactgc gggcgtgccg gaccccgatc tcatcattcg
     9841 cacgtccggc gaattgcgta tcagcaattt tctgatctgg caggccgcct attctgagtg
     9901 gtatgtgacc cccacttatt ggccagactt tggcaaggac gaatatcgtc gcgccctcga
     9961 aacattcgtt caacgggatc gtcgatatgg aaaggtcaaa tccaaagaat acgaggctac
    10021 caatgcttag gcgcacgctc acagcccttg tgctggtcgc cattggtttg cctggcatta
    10081 tttacggggg cgtgtattat tttgtgttga tcacaatttt tctagtcggt agcgcatggg
    10141 agtatgtgcg tttgtatcgg gcggttcagt atgaaccgca tgacatactc acgatcggag
    10201 gcgtgcttgc aatcgcaaca gcacgcgttt tttttgtcga attcaccctg gtattattcg
    10261 cattattgat actgcttgcg atgacagttc atcttgtagc atttgagcgc ggtcgagacc
    10321 aggctgcgct tgatttttgt gtgacggtgg caggcatcgt atatctcggc tggctgggtt
    10381 catatttgct ggacctgcgc aatctggatc tcggtgtctg gtggctcatg cttgtacttc
    10441 ccatcgtgtg gggcggagac acaggtgcat actccatcgg tgcggcgtac ggcaaacata
    10501 agatgacgcc tcgcctcagc ccgaaaaaaa gctgggaagg ctattttgcc ggtgtgttta
    10561 cctcgatgat catcggtgct tttttcgcct acgctttttc agcgttgggg ccacagccat
    10621 taaacggttt gattgatcca ttacgaggtg ggttactcgg actggtgatt ggtgcagttg
    10681 ttccgttggg cgatctcggc gaaagcatga tcaaacgcca aagcggcctc aaagattcga
    10741 gcaatatctt tcccggtcat ggcggctttc tggatcgcat cgactcgtgg atttggggcg
    10801 cggtgattgg ttatttcgct atacaacttt ttatcttgta gtggaggtgt tatggaattt
    10861 ggtagtgatc cctcacggaa tctcgcgctg gaattagcac gtgtgaccga ggccgcggca
    10921 ttagcggctg gtcgctttat gggtcgcgga gataaagagg cggcggacca ggccgcggtc
    10981 aacgcgatgc gtatcgtcct gcagacggtg gatatgaacg gcgtcatcat tatcggtgaa
    11041 ggcgaaaagg acaacgcgcc gatgttatat aacggcgaaa atgtaggcaa cggttctgct
    11101 cctgacgtgg acgttgcggt ggatccgatt gatgggactc gccctctggc atttggtcgt
    11161 tccaattccc tggcgactgt tgctattgcg ccgcgtggca ccatgtttga cccgggccca
    11221 tttgtctaca tgaacaagct cgccgtcgga cccgaggcaa agggtaagat cgatattgaa
    11281 aggtccatca cggataactt gaaagcaatt gcaaaggcca aaagaaaaga tgtggaggat
    11341 ttgaccacga tcattctgga tcgtgatcgc cacaaggata tggtcgcgga gatccgtaag
    11401 gctggcgcgc gcattcggca aattccggat ggtgacgttg ccgccgcgtt gatgactgcc
    11461 tggcccgatt caggtgtgga tgtcttgatt ggcgtaggcg gtacaccaga gggagtcatt
    11521 gctgcctgtg cactgagtgc tatgggtgga gagatccagg gcaaaatgta cgcgcgcgat
    11581 gaagatgaat tgcgccgcgg ccgcgaagct ggctatgact ttgacaaggt tctcacaatg
    11641 aacgatctcg ttgcttcgga agaggtcttt ttcgcggcga caggtatcac cgatggggaa
    11701 ttactccagg gtgtgcgtta tttcggcgat ggtgccagca cagactcgct tgttgtacgc
    11761 ggtttaaccg gaacagtgcg gcagatcacc gccacacacc gcattgataa actgcgccat
    11821 ctgagtgctg tgaagtatta atataatgag gagaatcgtc gtttgacggt tcttcttttt
    11881 tatgctatag ttatgaatac ttgagcaatt attcatatat ttaagatgtt actccaagaa
    11941 aacacctcca cccatctggc tgaccttttc agcgccctga gcgaccccac gcgcttgcgc
    12001 atcatttcag ttttgctgga tagcgaaatg aatgtagggg agatcgcgac tcagcttgaa
    12061 atgaccgagt cagccgtatc tcaccaactg cgtggactcc gtcacatgcg acttgtgcga
    12121 gcccgcaaaa atggccgtca ggtttactat gcccttgacg atgaccatgt ggcgaagctt
    12181 tatcgccttg ggttggagca cgtggatcac ggatagtttg tgcacacaca ctcccattct
    12241 cattcacatt tcggcgatat cgccaaacaa accacatcac gcatggcgat ttcactgaca
    12301 ctaacgctgg tctttgtgtt cattgaagcc tttgccggga ttgttggcaa cagcctcgcc
    12361 ttgcttaccg atgcggcaca taaccttact gatgtgatcg ctctgggttt gagttggttt
    12421 gctattcgtc tgaccaccca gccttccaac gcacaaaaaa cgtatgggta tcaccgtgca
    12481 ggcattctgg ttgccttgat taattccacg acactggttg taatctcgct cgggattttc
    12541 tatgaagcct ggcgtcgttt ctcgaatccg cccgaagtgc agtcagggat attgatcacg
    12601 gttggtctga tcgctgtcgt tattaacttg gtgaccgccc tgcttgtcag gaagggtagc
    12661 gaacacgatc tcaacattcg ttccgctttt ttgcacttga tgggagatgt ggtatccaca
    12721 gttggtgcgg ttattgccgg tgtcatcatc tactttaccg gtgcgaactg gctggatccg
    12781 ttggtcagcg tcttaattgg actacttatt ctttataatg cctggggcat tctgaaagac
    12841 gcaatcgata ttcttttgga agccacgccg cgtgacgtag atatgcaggc tatggtaaaa
    12901 gatatcgcga aagtagatgg tgtgataggt gttcacgaca tccacgtctg gagcctgacc
    12961 caaagcctgc gaaccatgtc tgctcatatc cttacggatg atctgccaat cagcgccggt
    13021 gctatgattc aaaatcaaat caacgaaatc ctctttcatc gttacaaaat tgctcacgca
    13081 acgcttcaat tggaatgcgt ggaatgttcg cctgattctc tgtattgcga tttaaatgga
    13141 catgtacacg ttggggatgt ctcaacgctc catcgagatg atgggcattc ctaggcaaat
    13201 cttcgcatca agcctcaagt tgacaccgtc taagtagaac tatcctcacc ttggcgttta
    13261 tccgttatca ttggcgcggc tggaacgact tcccgtttcg acgacttctt atgaaatatc
    13321 ttctgaaaat ttggcgtgtg ctccctctgt ggcttcagat tattttgtct cggttcatcc
    13381 gaccgctgtt tcaggtcttt gccgcgggag tgatcttcaa tgaagatgat gagattttat
    13441 tggttaaatc tacctaccaa aggtttcatc cctgggggtt gccaggcggc agcctggagt
    13501 atggggagag cgccgaagag gcagtcaaac gcgaagtctg ggaagagacc ggcttagtga
    13561 tagagatcaa aagactcttg ctcgtcaaaa catggtcgcc ggatcgagtc ggcatgtatt
    13621 atttgtgcga agttactggt ggtggaattc atcctacaga cgaagtgtcg gaagccggtt
    13681 attttgcttt ggatgatctg ccagatgtga gatctgagga tatcggcttg attaaagagt
    13741 tacacagaat gaccgattta caaagcctta ccatggaaaa ggttctacta gagacgaagt
    13801 tgtgaactaa tttgtgaaat caaatgaact ggcttgaagt atcccttaca gttaacggtg
    13861 aactcgccga agccgtagcc gatgtgctgg cgcgttttgc ttacagtggt gtgatgatgg
    13921 agcagggcgt caaatatacc gatgatgaag acgcgggcac ccccaccgga cccatcaccg
    13981 tccgcgcgta cctggaagtg aacgataatt tggaagagac ccggcaaaag cttgaagagt
    14041 ctctttatta tctcgggctg attcagccct tgcctgccgc ctcttataag gaaatcgccg
    14101 accaaaattg gatggaggca tggaagcagc actacaaacc tatccttatt ggaaagcgac
    14161 tcgtcattgt ccccgcg
//
LOCUS       DASTJP010000663         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_471438, whole genome shotgun sequence.
ACCESSION   DASTJP010000663 DASTJP010000000
VERSION     DASTJP010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471438"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VFQ23_19390"
     CDS             complement(<1..885)
                     /locus_tag="VFQ23_19390"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9908823.1"
                     /translation="MDDITTILQRVPQFSSAQISEIEELSGGLTNKNYKITVDDEHYV
                     LRMGGNETKYLGIDRGVEYECSRLAAQIGIAPEPVAFLEPEGYIVARFVSGKGIPAAE
                     TGNEENIRRVLESMKAYHALEHFPGSFSPFRVAEEYKKTAMSFDVKLPEKMDWYLEKS
                     REIETAMYGREPLKLSPCHNDLLNGNFIDEGTRIRILDWEYAGMGDIFFDLGNFAVQH
                     EFNDEQDEILLRAYFGEVTDSQRAHLKLMKIMSDLREAMWGMVQVGVSKLDFDYVGYA
                     QKYFDRYDANTSGDDYRRW"
     gene            complement(998..2026)
                     /gene="fni"
                     /locus_tag="VFQ23_19395"
     CDS             complement(998..2026)
                     /gene="fni"
                     /locus_tag="VFQ23_19395"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET9908824.1"
                     /translation="MSKVAPIEQRKADHIKINLEKDVRSALTTGLENYHFMHEALPEL
                     DLDHVDTSLKLFDKELAAPILISSMTGGTEEAETINLRLAETAYVMRVAMGVGSQRAA
                     IEHPEQAKTFQVRRVAPDILLFANLGAVQFNYGYGVDQCRRAVEMIEANALYLHLNPL
                     QEAVQAGGDTNFAGIAKKIEEVCRKLEIPVIAKEVGWGISERTARLLANCGVSAIDVA
                     GAGGTSWSQVEMHRAPDEFTRELAATFVGWGIPTADSILNVKKAAPNMTVFASGGIKD
                     GLDIAKCVALGASLGGMAGSFLKAAAVSSDKAVEVMKLVKRQIQVTMFAAGVETLDGL
                     KAGKLFRK"
     gene            2115..2348
                     /gene="acpP"
                     /locus_tag="VFQ23_19400"
     CDS             2115..2348
                     /gene="acpP"
                     /locus_tag="VFQ23_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9908825.1"
                     /translation="MSDTYEDVKAIIKDLLGADEAKITPEARFREELEADSLDLVELI
                     MAFEDKFGAEISDEDAQKIVTVGDAVTYIDSHK"
     gene            complement(2601..>3397)
                     /gene="sucD"
                     /locus_tag="VFQ23_19405"
     CDS             complement(2601..>3397)
                     /gene="sucD"
                     /locus_tag="VFQ23_19405"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET9908826.1"
                     /translation="HTTQMVSYRTNVVAGVTPGKGGEWVLDGKIPVFDSVKLAADATG
                     ANCTVIFVPARFAPDAMFEAADAGIPLIVCITEGVPVQDMMRVRNYLDQKKVRLIGPN
                     CPGMLTPNESKVGIIPGNIAIAGNVGVVSRSGTLTYEVLYALKQVGMGASTCVGIGGD
                     PVNGTNFIDVLEMFEHDPGTEKVVLIGEIGGTDEDKAAEFISDRMTKPVVSFIAGSSA
                     PPGKRMGHAGAIIEGGAGSAADKVRALEAAGVKVAQHPEEIPSLLN"
BASE COUNT          798 a          901 c          809 g          889 t
ORIGIN      
        1 ccaccttcga taatcatccc cacttgtgtt tgcgtcatat cggtcaaaat acttttgagc
       61 ataccctaca taatcaaaat ccaacttgga tacacccacc tgcaccatgc cccacatcgc
      121 ctcgcgcaga tcggacatga tcttcatcag tttgagatgt gcgcgctgcg agtctgtcac
      181 ctcgccaaaa tatgctcgca ataatatttc atcttgttca tcattgaact catgctgaac
      241 cgcaaaattt cccagatcaa agaagatatc gcccatgccc gcgtattccc aatccaaaat
      301 gcggatgcgc gtgccctcgt caatgaagtt gccgttgagc agatcgttat ggcaagggct
      361 tagcttgagc ggttcgcgtc cgtacatcgc ggtttcgatc tcgcgtgatt tctcaagata
      421 ccaatccatt ttttctggca gttttacatc gaagctcata gcggtttttt tgtattcttc
      481 tgcaacacgg aaaggagaga aggatccagg gaagtgttcc agggcgtgat aggctttcat
      541 cgattcaagg acacgcctga tgttttcctc gttgcctgtt tctgcagcgg gaattccttt
      601 ccctgaaaca aaccgcgcca caatataacc ttccggctca agaaaagcga ccggttcagg
      661 agcgatcccg atttgcgctg ctaaacgact gcattcgtat tcaaccccgc ggtcaatgcc
      721 aaggtatttt gtttcatttc ctcccatgcg caggacataa tgttcgtcat cgacagtgat
      781 tttgtaattc ttgttggtga gtccgccact taattcttcg atttcggaga tttgggcaga
      841 ggaaaattgt ggaacgcgct ggagaatggt agtgatgtcg tccatgagga atccttggtg
      901 aagatatggt tattagatta accctccaca atcaagtaag tttttatttg tttcgcaatt
      961 ttgattagct actaaaggtc atcaatagaa tcttgaatta ctttctgaat aatttcccgg
     1021 cctttaaacc gtctaaggtt tcaacacccg ccgcgaacat cgtgacttga atttgcctct
     1081 ttaccagctt catcacttcc acagccttgt cgcttgacac tgcagcggct ttcaagaagc
     1141 tccctgccat gccccccagt gacgcaccca aagccacaca cttggcaata tcaaggccgt
     1201 ctttgatgcc gccgctggcg aagactgtca tattgggcgc ggctttcttc acatttagaa
     1261 ttgaatctgc agtgggaatt ccccagccaa caaaggtcgc ggcaagttcg cgcgtgaatt
     1321 cgtccggcgc gcggtgcatt tccacctgag accaactcgt cccgcccgcg cctgccacgt
     1381 cgattgcact gactccgcag ttagctaaga gccttgctgt acgttctgag attccccagc
     1441 ctacttcttt tgcaatgact gggatttcga gcttccgaca aacttcttcg attttttttg
     1501 cgatgcctgc aaagtttgta tcaccgcctg cctggaccgc ctcctgcaac ggattcagat
     1561 gcaggtagag cgcgttggct tcgatcatct ccactgcgcg gcggcattgg tcaacgccat
     1621 agccataatt gaattgcacg gcaccgagat ttgcaaataa caaaatatcg ggcgcgacgc
     1681 ggcgcacttg aaacgtcttg gcttgttcag gatgttcaat cgccgcgcgc tggcttccga
     1741 ctcccatcgc gacccgcatt acataggcag tctcggcgag tcgcaggtta atggtctctg
     1801 cttcttctgt cccgccggtc atggaactga tcaggatggg agctgccaat tctttgtcga
     1861 acaacttgag gcttgtgtca acgtgatcca aatctaattc cggcaaagcc tcatgcatga
     1921 agtggtaatt ctcaaggccc gtcgtcagcg cggagcggac gtccttctcc aggttaattt
     1981 tgatgtggtc agcttttctc tgttcaatcg gcgcaacttt tgacatgggg cagtgttcct
     2041 gtgattgtaa aaatgacttg acgtggtcgt tttaccgagt aaaattgcca cgaaaatcta
     2101 atgaggaggt tggtatgtct gacacatatg aggatgtcaa agcgattatc aaagacttgc
     2161 ttggtgcgga tgaggcgaag attactcctg aagctcgttt ccgtgaggaa cttgaggcag
     2221 attccctgga ccttgttgaa ctgatcatgg cttttgaaga caaattcggc gccgagattt
     2281 cagacgaaga tgcccagaag atcgtgaccg tgggcgacgc cgtcacgtat attgactcgc
     2341 acaagtaaac tttattttga ttataaccgt agagccgtcc acaaggacgg ctctatcaat
     2401 ttattgagta agtcggtggt tgagtagcct gaggcgacag ccgaagacat atcgaaacca
     2461 ccagtttgta agtaatcttt tgtctttgtg ttaccggttg gtctcgatac gggctcacct
     2521 gcactgcacc agccccagtg cggtgcaagt gtcgcaaaaa acgctcagac ctactcgacc
     2581 agcagctcct cgcaattaca ttaatttagt aaagacggaa tctcttccgg gtgttgagcc
     2641 actttcacac ctgcggcttc gagcgctctc actttatcgg cagcggaacc tgcgccacct
     2701 tcgatgatcg caccagcatg tcccatccgt ttgccgggag gggcagatga gccggcgata
     2761 aacgaaacca caggctttgt catccggtcg gaaatgaact cagcggcttt gtcttcgtcg
     2821 gttccaccga tctcaccgat cagaacaacc ttttcagttc ccgggtcgtg ctcgaacatt
     2881 tcaagcacgt cgataaagtt tgtcccgttg accgggtcac cgccgatgcc aacacaagtc
     2941 gaagcgccca ttccaacctg tttcaacgcg tagaggactt catacgtcag cgtgccggag
     3001 cgtgacacca cgccgacatt ccctgcaatg gcaatgttgc cgggaatgat cccaactttt
     3061 gattcattag gggtcaacat gcctgggcag tttggtccga tcaggcgcac tttcttttga
     3121 tcgaggtaat tgcggacgcg catcatatcc tgtacaggga caccttctgt gatgcaaaca
     3181 atcaaaggga tgcctgcatc cgcagcttcg aacatcgcgt cgggagcaaa gcgggcagga
     3241 acgaaaatca ctgtgcaatt cgcgccagtg gcgtcggccg cgagcttgac cgagtcaaac
     3301 acaggaattt ttccatccag aacccattca ccgcctttgc ccggagtcac accggcgaca
     3361 acattggttc tataggaaac catttgcgtg gtatgga
//
LOCUS       DASTJP010000664         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1029809, whole genome shotgun sequence.
ACCESSION   DASTJP010000664 DASTJP010000000
VERSION     DASTJP010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029809"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFQ23_19410"
     CDS             complement(<1..370)
                     /locus_tag="VFQ23_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908827.1"
                     /translation="MTTKKQFISKLLQERDKFELLLNRVGYSRRMTLKGVSGKWSIKD
                     VLAHILAYEQYIADRMNEILHGEQYTPCRTQTALDAFLDEFGYPDFGSSLLDDDGPNA
                     WIVEKYQRVSLEDVVSQELQA"
     gene            complement(458..1378)
                     /locus_tag="VFQ23_19415"
     CDS             complement(458..1378)
                     /locus_tag="VFQ23_19415"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908828.1"
                     /translation="MKRYFVRPAKPEDIEAVHDLVAKQHAADYGQALRTLDDLRNIWQ
                     NINIETDTCTAHADGHLAGYAELLEGDSPYIYLAERGNVDLAFQLLRILEDKAVGHRA
                     EKVKLFTKISERNNTLLQLFASNGYRSNLSFIMMELEMDAPPPAPEPVEGITIRTFVR
                     GQDEQGTYQADEEAAEDKGYHTPLSFEDWSKRMRMEKESFDPTLWFLAFEGNEIAGAA
                     LNVYDRETNLGWVDHLSVRRAWRRRGIGRALLLHSFGEFYRRGIRRVKLNVDSKSLTN
                     APRLYEQVGMKTIQQYHIYTKEIDGVPPRF"
     gene            complement(1397..>2183)
                     /locus_tag="VFQ23_19420"
     CDS             complement(1397..>2183)
                     /locus_tag="VFQ23_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HET9908829.1"
                     /translation="NKYISDEDIINTTREIYARGWTTIKLYFMIGHPSETLEDVQAIA
                     DLCKRVITEGRKVSGWKVKLNAGVSTFVPKSQTPFQWVSCDTPDQIKAKQALLRRELS
                     DKNIKVSWTDAEDSFLEAWLSRGDRKMAEVVYTAWKNGARFDAWHETGSYKKWITAFE
                     EHGLDPIFYTHRQRRTDEVFPWEHITAAVRKNFLFQDFRQSLEGQIRVDCRLDCFACG
                     ILPTFADMRRENPGDVWKCPDVKSPASSNPYAMSSLKLPMVGD"
BASE COUNT          532 a          571 c          481 g          599 t
ORIGIN      
        1 acgcctgcaa ttcctgcgag accacatctt cgagagaaac ccgctgatac ttttcaacaa
       61 tccaggcatt tgggccatca tcatccaata gagaagagcc gaaatccgga taaccgaact
      121 catccaggaa agcatccagg gccgtctgcg ttctgcacgg cgtatattgc tcaccgtgta
      181 agatctcgtt catgcgatct gcaatatatt gttcgtatgc gagaatatga gccagaacat
      241 ccttgatgga ccactttcct gatacgccct tcaacgtcat gcgacgcgag taaccaacgc
      301 gattcaagag taattcgaac ttatcacgtt cttgtagcag ctttgaaatg aattgtttct
      361 tagtcgtcat gctctttatg ataccctgtt ctacttcctg cttcaatgaa taccaggctt
      421 gtaaactgat ccgcaggtta taagtctaat gtccagtcta aaacctgggc ggcactccgt
      481 cgatttcttt tgtataaatg tgatactgtt gaatcgtttt cattccgacc tgttcataaa
      541 ggcgaggggc attggtcagg ctttttgagt ctacattaag ttttacacgc cgaatcccac
      601 gtcgatagaa ttctccaaag gagtgcaaaa gtaacgctct gccgattcct cgcctgcgcc
      661 aggcgcgtcg tacactcaga tgatccaccc agcctaggtt agtctcacga tcatacacat
      721 tcaaagcggc tcccgcaatc tcgtttcctt cgaatgccag gaaccataac gttggatcaa
      781 atgattcttt ctccatgcgc atacgctttg accaatcctc aaagctaagc ggcgtgtgat
      841 aacctttatc ctctgccgct tcctcatcgg cctggtaggt tccctgttca tcctgtcccc
      901 tcacaaatgt tcgtatggta atcccctcca ccggttctgg cgctggaggt ggcgcgtcca
      961 tttccaattc catcatgatg aaggatagat ttgatcgata tccattggat gcgaaaagtt
     1021 gtaagagagt attattccgt tcactaattt ttgtaaaaag tttgactttt tctgccctat
     1081 gcccaacagc tttatcttcc aggatgcgca gtaattggaa ggcaagatcc acgttgcctc
     1141 tttcagcgag ataaatatac ggagaatcac cttcgagcag ttcagcgtaa ccagccaaat
     1201 gaccatctgc gtgggctgtg caagtatcgg tttcaatatt aatgttttgc catatatttc
     1261 taagatcatc caatgtcctc aaggcttgac cgtaatctgc tgcatgttgc tttgcaacaa
     1321 gatcatgcac agcctcaata tcctctggtt tggcagggcg tacaaagtat cgcttcatgg
     1381 gatgatgacg cataatttaa tcccccacca taggtaattt caggctggac atggcataag
     1441 ggttagagga agcgggcgat ttcacatccg ggcacttcca tacgtcaccg ggattctcgc
     1501 gcctcatatc cgcaaaggtt ggcagaatgc cacaggcaaa acaatccagg cggcagtcca
     1561 cgcgaatctg cccttcaagc gactgcctga aatcctgaaa gaggaaattt ttacgcacgg
     1621 ccgccgtgat gtgttcccag ggaaaaactt catccgtgcg gcgctgacgg tgtgtgtaaa
     1681 agatcgggtc gagtccgtgc tcttcaaacg cggtgatcca cttcttgtaa ctgccggtct
     1741 catgccaggc atcgaatctg gcgccgttct tccaggcagt gtaaacgacc tctgccattt
     1801 tcctatcacc acgcgaaagc caggcttcta agaacgaatc ttcggcatcg gtccaactca
     1861 cttttatatt tttatcgctc agttcgcgcc gcaacaacgc ttgcttggct ttaatttggt
     1921 ccggtgtatc acaggaaacc cactggaagg gagtctgtga ttttggtacg aacgtgctga
     1981 cgcccgcgtt caacttgact ttccaccccg agacttttct gccttccgtg atgacgcgct
     2041 tgcacaagtc cgcaattgcc tgcacatcct ccagcgtttc actgggatgc ccgatcatga
     2101 aataaagctt gatggttgtc cagccgcggg catagatctc acgcgtggtg ttgataatat
     2161 cctcatcaga aatatatttg ttg
//
LOCUS       DASTJP010000665         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_688794, whole genome shotgun sequence.
ACCESSION   DASTJP010000665 DASTJP010000000
VERSION     DASTJP010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688794"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..413
                     /locus_tag="VFQ23_19425"
     CDS             72..413
                     /locus_tag="VFQ23_19425"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9908830.1"
                     /translation="MALFSIETDSTQSVSVMKVKGRVDSDTAPELDDALTKLLQDNKN
                     KIVLNLQAVDYMSSAGLRAMVKAYQNAKKSGGDVRLASVSTPVEVILRTVGMMQMLQM
                     YPTDQEAMASF"
     gene            435..830
                     /locus_tag="VFQ23_19430"
     CDS             435..830
                     /locus_tag="VFQ23_19430"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET9908831.1"
                     /translation="MWKTNDAEVKKILYVQTHGSATPERTATPFFLATAGAAMDNEVC
                     IYFTMNGPQCLEKGAPEKIVVPRKGGGGKELKYFIDQALEMGVRLFVCQPSLDLHGFT
                     MADMIEGVEMIGGAAFNDMAVRADAVISF"
     gene            969..1676
                     /locus_tag="VFQ23_19435"
     CDS             969..1676
                     /locus_tag="VFQ23_19435"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9908832.1"
                     /translation="MPTTLENWNAGKAEDRHPDVTYEEKERLFLEVKNDIRYSDFLYG
                     CYECGICVSACPSARFYDFSPRVIAQAIAREDVERVYDIITEDIWNCAQCFSCVRCPR
                     QNNPGGLVTILREVSLKNGLQESKDALQGYSRVIYKVMLKGNQVSPDMLQPDFFPDWG
                     PWVKNFSENMHVWRKAIPMDSIRGLTDVAWRTDRKTLLELYTIWFESGNMEIIKELDE
                     GLYDLLSDVMQEELEEG"
     gene            1761..2984
                     /locus_tag="VFQ23_19440"
     CDS             1761..2984
                     /locus_tag="VFQ23_19440"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET9908833.1"
                     /translation="MADSTFTPVEQILEKKSHPVTRDAEVAPTQDMHETLFELEAKGE
                     IIVQKVPEPYIEFTTKYGRNKKIPIEHTWHHKSCGHCGHIPGYASSILWLHRQFGLDY
                     HDPTDQTSCTGWNYYASGASNAVGQAAVMARNFAAAYETGYFPMIHCGTSFGHYKEIR
                     EQLVHHKELRDEVRRILGKMGKPLVIPEEIVHYSEWVYAMRHRFAERQLVDMSSIKAT
                     VHPACHYYKIVAEDAIYDPEIYGGQRTAVVTAVLESLGINVEDYSTWFDCCGFGFRHV
                     LVQRDFTRSFAVLRKIEVMKNEVNPDLTVTHDTGCVTTLDKSQFAAKAHDRNVGIPVL
                     SDAQVAALAMGAHPFRVVQFHWHSTDWRPFLEKLGINWQKYWDEFQGDLDAIRAGTKS
                     GITWQDADQPIALTA"
     gene            3135..>3613
                     /locus_tag="VFQ23_19445"
     CDS             3135..>3613
                     /locus_tag="VFQ23_19445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9908834.1"
                     /translation="MTSGKVLIIGGGPAGLEAARCVAELGGEAILIEKRERLGGTPDR
                     ENYAKLTHHWRDASEAMAELSASVTGNPNVEVKYQAEVRSLSGDEGDFEVQIESGGVA
                     ESINIGAVIVATGFQHFDPGRATQYYNYYSFPDVITLTDLEKMLRDHNVVRPSNGEVP
                     "
BASE COUNT          930 a          892 c          953 g          838 t
ORIGIN      
        1 gaggcgcggc aactttcctg gaatatgccg cggatgcgga tgttcagctg ttcatttaat
       61 gaggcgttga aatggcactg ttttcaattg aaaccgatag tacacaaagt gtatctgtca
      121 tgaaggtaaa gggtcgggtc gattccgata ctgcacctga attggatgat gcacttacaa
      181 aacttctgca ggacaacaaa aacaaaattg tcctcaatct acaggctgtc gactatatgt
      241 catccgcggg tttgcgggca atggtcaagg cttatcaaaa tgcaaaaaaa tctggcgggg
      301 atgtccggct tgcttccgtc tctactcctg tggaagtcat actacgtacc gttggcatga
      361 tgcagatgct gcaaatgtat ccgaccgatc aggaagcaat ggcaagtttc taggtattgt
      421 cctggagggc actcatgtgg aagaccaatg acgctgaggt gaaaaagatc ctgtacgttc
      481 agacgcacgg cagcgcgacg ccagagcgga ccgcgactcc tttcttcctg gcgaccgctg
      541 gcgcggcgat ggataacgaa gtctgcattt acttcacaat gaatggaccg cagtgtctag
      601 agaaaggcgc gcctgaaaaa atcgttgtgc cgcgaaaagg gggaggcgga aaagaactca
      661 aatattttat tgatcaagca ttggaaatgg gtgtgcgctt attcgtttgc cagccttcgc
      721 tggatttgca tggctttacg atggccgaca tgattgaagg cgttgaaatg atcggcggcg
      781 ccgcgttcaa cgatatggct gtccgtgcag acgcggtgat ttctttttaa acacgaagga
      841 cacgacacct gccctggcgg gcggtgccag ggtaagccgc acggtgccag ggagtacaca
      901 aagatctaaa gatcttacct tcgtgttcct tggtgacctt tgtgttgaat cgttttcagg
      961 aggcacacgt gccaactact ctcgaaaact ggaatgcagg caaagccgaa gatcgtcatc
     1021 cggatgtgac gtatgaggaa aaggaacgcc tcttccttga ggtaaaaaac gatatccgtt
     1081 acagcgactt cctatacggt tgctatgaat gcggaatctg tgtctccgct tgtccatcgg
     1141 cgcgtttcta tgatttcagt ccacgcgtca ttgcacaggc catcgcgcgc gaagacgtgg
     1201 agcgtgttta cgatatcatc acagaagata tttggaactg cgcgcaatgt ttttcgtgtg
     1261 tgcgctgtcc acggcaaaac aatcccggag ggctggttac tattttgcgc gaggtatccc
     1321 taaagaatgg attgcaggaa tcaaaagacg ctttacaggg ctacagccgt gtaatctaca
     1381 aagtgatgct caaaggcaac caggtctcgc ccgatatgct ccagcctgat ttcttcccgg
     1441 attggggtcc atgggttaaa aactttagcg agaacatgca tgtctggcgc aaagccatcc
     1501 ccatggattc catccgcggt ctcaccgatg tcgcctggcg gacagaccgc aagacgttgc
     1561 tcgaacttta caccatctgg ttcgagtccg gcaatatgga aattatcaaa gagctcgatg
     1621 aaggtttgta tgatctgctt tcagatgtca tgcaagaaga gctggaagaa ggatagacga
     1681 aagaagatcc ctgcaaactt cacccataac cttatgctgt tttattgata gccttgtcaa
     1741 ctgaacgatt ggagaaatga atggctgact caacctttac acccgtcgaa caaatcctcg
     1801 agaagaaatc gcatcccgtt acgcgcgacg cggaagtggc gccaacgcag gacatgcatg
     1861 agactttatt tgaacttgaa gcgaagggtg aaattatcgt tcaaaaagtt ccagaaccat
     1921 acatcgaatt tacaacaaaa tacggacgta ataaaaaaat cccaattgaa cacacctggc
     1981 accacaagtc ctgcggtcac tgcgggcaca tccccgggta tgcctcgtcc atcctttggc
     2041 ttcatcgcca gtttggcctg gattaccacg accccaccga ccagacttcc tgtacaggct
     2101 ggaactatta cgcctccggg gcatccaatg cggtgggaca agctgcggtg atggcacgca
     2161 acttcgctgc tgcctatgag acgggttatt tcccaatgat ccattgtggc acaagttttg
     2221 gacattacaa ggaaatccgc gaacaacttg tgcatcataa agaactacgc gacgaagtcc
     2281 gtcgtatcct cggcaaaatg ggcaagccgc tggtgatacc tgaagaaatt gttcattaca
     2341 gtgaatgggt ctatgccatg cgtcaccgat tcgccgagcg tcaactcgta gacatgagtt
     2401 ccattaaggc aacggttcac ccggcctgcc attattacaa aattgtcgcc gaagatgcaa
     2461 tttatgaccc cgaaatctat ggcggacagc gcactgcggt tgtgactgcg gtgcttgaat
     2521 ctctgggtat caacgtggaa gactattcca cctggttcga ttgctgtgga tttggtttcc
     2581 ggcacgtctt ggttcagcgc gacttcacac gttcatttgc tgtacttcgc aaaattgaag
     2641 taatgaagaa cgaagtcaat cctgatttga ccgtgacgca cgatacaggt tgtgtgacca
     2701 cgctcgacaa gagccaattc gctgcaaaag cgcatgaccg caacgtgggc atccccgtgt
     2761 tgtcagatgc gcaggttgcc gcactggcga tgggcgcgca tccgttccgc gtggtgcagt
     2821 tccactggca ctccacggac tggcgtccct tccttgagaa acttggaatc aactggcaga
     2881 aatactggga tgaattccag ggcgatctcg acgccattcg cgctggcacg aaatctggaa
     2941 ttacctggca ggacgccgat caaccgattg cgctgacggc gtaaggcttg catgtcgctg
     3001 cgaggagtcg ctcttcccaa agaagcaatc ttcgcctaaa cggcggattc ctctcgatga
     3061 gattcatcca caaaacggga gattgcttcg ccaaagaacg gctcgcaatg acatatcaac
     3121 atctggagga atacatgaca tctggcaaag tgctcatcat cggcggggga ccggcggggt
     3181 tggaagccgc gcgctgtgtt gcggaattgg gcggggaagc cattcttatc gaaaaacgtg
     3241 aacgactcgg tggaacacct gaccgtgaga attatgccaa gctcacgcat cactggcgcg
     3301 acgcgtccga agcgatggcg gaactctccg cgtcggttac gggcaacccc aatgtggaag
     3361 tcaagtatca agccgaagta cgaagtttgt ccggagacga gggcgatttc gaggtccaga
     3421 ttgaatccgg tggcgtagct gaatccatca acataggcgc ggtaattgtt gccacgggct
     3481 tccagcattt cgaccccgga cgagcaacgc aatattacaa ctactattcg tttcctgatg
     3541 tcatcacact gacggatctg gaaaagatgc taagagacca caatgttgtg cgcccttcaa
     3601 acggtgaagt gcc
//
LOCUS       DASTJP010000666         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1111721, whole genome shotgun sequence.
ACCESSION   DASTJP010000666 DASTJP010000000
VERSION     DASTJP010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111721"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..503)
                     /locus_tag="VFQ23_19450"
     CDS             complement(15..503)
                     /locus_tag="VFQ23_19450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9908835.1"
                     /translation="MQVLIKPTMIIKEWSDEQLIAQVAHGDTAAYEMLYDRYSSVVMG
                     FALKITGDRSLAEEILQETFWRVWRKADLFQNQRGAFTSWFFGIARNLSIDMLRRQRT
                     QVQPADESDQMIERTADPALDVSETAWLRVKYQQMKAAMESLPNEQRYVIEMAYFRGM
                     TR"
     gene            626..967
                     /locus_tag="VFQ23_19455"
     CDS             626..967
                     /locus_tag="VFQ23_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908836.1"
                     /translation="MTEVLYGPRLGKEGKIRVGCSAVIFDETRTKVLLMVGVHSNPDQ
                     LVIYPDGTKVHIVLLGFEAEVISGELSLSDETTDVGFFLFAEMESRHMHGKHKERVDD
                     AFSNQIEAFIK"
     gene            1076..>2034
                     /locus_tag="VFQ23_19460"
     CDS             1076..>2034
                     /locus_tag="VFQ23_19460"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET9908837.1"
                     /translation="MFGGIETDQLYLFLILIGAILLLFTEWIRIDLTAILIILMLTIT
                     EVLEPEAAFSGFSSEPAILLAAVFVLNGALYHTGLSEQLGNWIKRLAGKSFEQAIGVV
                     MPSVALLSAFTHHVTITALMLPPTLKLARENNIPASRLLIPLSFAASLGTAITIIGAP
                     AFLISDGLLKQAGQSGLGIFSIAPIGLALSLAGTIFFLLLGRFLLPARRGDEEAIDHF
                     RLEGYYTELVILPDSPFIGKTVREIEEGQPTDFKISAWYRDDRPRNRPFGSKKAQAGD
                     VLVVRTNPDKLATIQQEPGIALRPLQKYEETLLLSQENENNKE"
BASE COUNT          512 a          480 c          501 g          541 t
ORIGIN      
        1 gctcgggcaa tctcctaccg ggtcatgccg cgaaagtaag ccatctcaat gacatagcgt
       61 tgttcattgg gcaggctttc cattgcagcc ttcatctgtt gatacttgac cctgagccat
      121 gcagtttcag aaacatcgag agctggatct gccgttcgct caatcatttg atctgattca
      181 tctgcaggct gcacttgagt ccgctgccgc cgcagcatgt caatggatag gtttctggca
      241 ataccaaaaa accaactggt aaacgcacca cgctggtttt ggaacaggtc tgccttgcgc
      301 catacccgcc agaatgtttc ctgaagaatt tcttccgcca gggaacggtc tccggttatt
      361 ttcaatgcaa accccatgac tactgagcta tagcggtcat aaagcatttc atacgcggcg
      421 gtatcgccgt gtgccacttg cgcgatcaat tgctcgtcgc tccactcctt aataatcatg
      481 gttggcttaa tcaggacctg cattcatatt agtatacgca cccggcagca aagtgaatct
      541 atgatgagtt aggggcatta tacttgaaca caatcacgtg gttacaggtt ccattgatga
      601 tgtggaaagg gagatttatg aaattatgac tgaagttctt tacggtccac ggcttggcaa
      661 ggaaggtaag atacgcgttg ggtgttcggc tgtcatcttc gatgagacgc gcacaaaagt
      721 attactaatg gttggagtgc atagcaatcc tgatcagctg gtcatttatc ctgatggaac
      781 gaaggtccat attgttttgc tgggatttga agcagaagtc atcagtggcg aattgagctt
      841 gagcgacgag accaccgatg taggtttttt cttgttcgcc gaaatggaat ccaggcacat
      901 gcatggcaaa cataaggagc gcgtggatga cgcgttttca aatcaaatcg aagcattcat
      961 aaagtaaatc gttgtcgaag attacttcag atattactgc tctcagcggc ttgaacaagt
     1021 atggtaggga ttttttgtat ttcacctaat cagcaaaaaa atatataatg gttgaatgtt
     1081 cggtggtatt gaaacagacc agctctattt attcctcatt ctgatcgggg caatacttct
     1141 gctgttcaca gaatggatcc gcattgactt aacggcaatt cttattatct tgatgttgac
     1201 catcacagag gtcctcgagc cggaggctgc cttctctggt ttcagcagtg agccagccat
     1261 tttgctggca gcagtatttg tgctcaatgg ggctttgtat cataccgggc tgtctgagca
     1321 attggggaac tggataaaac gactggcagg caaaagcttt gaacaggcaa ttggcgtggt
     1381 gatgccctcc gtcgcattgt tgtcagcttt cactcatcac gtgacaatta cagcgctcat
     1441 gctgccgcct acgctgaagc tggcacgtga aaataacata cccgcctcca gactgttaat
     1501 ccccttgtcc tttgcagcat cgctgggcac tgcgattacg atcataggcg cccccgcttt
     1561 tttgatttcg gatggtctgt tgaagcaagc agggcaaagt ggtttgggaa tcttttcgat
     1621 tgctccgatc gggctggcat tgtcactggc gggaacgata ttcttcttgc ttcttggaag
     1681 attcctgctg ccagcgcgcc gcggtgatga agaagccatt gatcattttc gtctcgaggg
     1741 ttattacacc gaattggtca tcctgccaga ttcaccgttt atcggcaaaa ccgttcgaga
     1801 aattgaagaa ggacaaccaa cggactttaa aatatctgcc tggtatcgcg atgaccgtcc
     1861 acgcaaccgc ccgtttggca gcaagaaagc ccaggcaggg gatgtgctgg tggtccgcac
     1921 aaacccggac aaactcgcaa ccattcagca ggaacccggc atcgcactcc gccccttaca
     1981 gaagtatgaa gagacattgt tactttcgca ggaaaacgaa aacaacaagg agga
//
LOCUS       DASTJP010000667         6950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_673224, whole genome shotgun sequence.
ACCESSION   DASTJP010000667 DASTJP010000000
VERSION     DASTJP010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673224"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFQ23_19465"
     CDS             complement(<1..269)
                     /locus_tag="VFQ23_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908838.1"
                     /translation="MYKKKIFNRRSPSTRSFRLPLGLVLVVLAITLCILIPSTPMNLS
                     GINVSDRSLPTPFPTFTPGPLPTKEHIGRIIYTCTRGDFNQLCSV"
     gene            complement(509..2935)
                     /locus_tag="VFQ23_19470"
     CDS             complement(509..2935)
                     /locus_tag="VFQ23_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908839.1"
                     /translation="MQIHRKLHRMIQTVFIFLFAAVVLPAQSMVASAGELQQPTARDQ
                     ALSFPPDPTADIPWSAGTSTVAHIQAAFNNARTVENAQLGSSIPMMTLPSQSEWDGMT
                     DGQKALWLINRERIDRGVAPLQGIEANVMGVAQYYADYLLDNNTWGHTADGRTPWQRL
                     EDNPAIGACHDFLSVAENIFVFVTSGSSISLPLERAIYGWMYEDGPCCSWGHRHAILW
                     YPYNDNSGTVGSEGFLGIGRANGGPYQGPFSQSWNFAEMIVMNVFDPCASWNDAPPVV
                     NSITRLDPNPTSASTVRYNVTFSESVTGVGTNDFTLNNTGGISGASVSTVSGSGSTRT
                     VTVNTGTGTGTLRLVVSDNDSIVDASGNPLGGGGAGNGDFTSGEIYDIVLPNTNVFIG
                     GALIEDHYIPTPGSLRVGYPGVDDGPVQVVSTGTSNTLAALRVIWREPGLRTSYSEMM
                     GLPKEQLSTEYWFPWYNNLDTNSMDQGFRIANVDTTTHIIRVFLGTTQIGGDISLDGG
                     GSTRVGYSVNNGPIHIVCVTCNPANTDDKIIAALRVIWQEPGSRFSYSEMMGLPKEQL
                     STEYWFPWYNNAVANSMDQGFRIANVSPAETNTIEVWAGNDLLESFSLDAGASRRVGY
                     NVDNGPVRVTCTTCTNTDNDQILAALRVIWKEPGFRSSYSEMMGLPKEQLSDEYWFPW
                     YNNAVVNSMDQGFRIANVSPNETNTVEVWVDGNKVDTIILNAETSTRVGYNVDNGPIR
                     VVCTTCTITPYDQILVALRVIWKEPGYRSSYSEMMGLPVEALSTQYWFPWYNFAVPNS
                     MDQGLRISIP"
     gene            complement(3134..3388)
                     /locus_tag="VFQ23_19475"
     CDS             complement(3134..3388)
                     /locus_tag="VFQ23_19475"
                     /inference="COORDINATES: protein motif:HMM:NF042904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6799 domain-containing protein"
                     /protein_id="HET9908840.1"
                     /translation="MNLNEGSVRMEAGRMMIVRNGELVVMDEGVLMNDGTRVLIDGMV
                     IRTDGTTRMMMDGESIGIDGEVTDREFQEEMEDNEARDDM"
     gene            3685..4113
                     /locus_tag="VFQ23_19480"
     CDS             3685..4113
                     /locus_tag="VFQ23_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908841.1"
                     /translation="MSFLLSNRATNTLQTLSKQTWSRMLSLARFYGWQPMGTLPPQTH
                     HLRKPAYEHGTKNSWDGTYLRNEGQIVRAEDALLLAMALELSLDDIPDVNPERGGASS
                     NAQDEPTPLGILPFEYFAGDGKQNLADFIRFCTLGEFIIL"
     gene            complement(4263..5786)
                     /locus_tag="VFQ23_19485"
     CDS             complement(4263..5786)
                     /locus_tag="VFQ23_19485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9908842.1"
                     /translation="MNIGRYEVIQELGQGGMAMVYLARDPYIKRQVAIKVLPRQFTFD
                     PQFRTRFQHEAEVIAALEHPAIVPVYDFGEVEDQPFIVMRYMPGGTLADRLTEGPLPI
                     PEIAALFERIGSALDHAHHMGVIHRDIKPGNILFDSQHVSYLSDFGISKIVESTAAIT
                     GTGIIGTPAYMSPEQAQGEKKLDGRCDIYSLGVVLFQALTGELPYKADTPMGQAMAHI
                     IEPVPSLLERRPDLPPTFERIIRKTLDKSPDRRYQTASQLSQAIHEGENEPEIPVVTS
                     AKDMAEVQVKTAEQQAPAEAVHTSQPLAGPASLVKSKNARMGLFGVSGFSILTLCLCA
                     GLAGGMASGLLPNPFRGPAAEALPSPRAESSELMLTSEVPVLSSDLSTVTLTVEASTP
                     TLSSSATPALATSFTPTQLHEEHLPYFTASTNMLCREGPGTEYVDRWQLNAGEVVQVL
                     AQWHQDSNWLLVDINVASSVTRTDCCWVGAEGTLNVPLEQIRMIDFLPDRLDCSAVR"
     gene            5917..6597
                     /locus_tag="VFQ23_19490"
     CDS             5917..6597
                     /locus_tag="VFQ23_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908843.1"
                     /translation="MGRQLTNLTFEEWLTFVFDHPVPTGQEKAWYWEFDADEWDETHA
                     DVIQFLTQAFENAEVVFQPYTDAQLNQGLWYIADNSCSNHMFALLDTNIPWNERQRCV
                     DSIHQLYEQCFAKRCSPHLSHIDEPGANPLNAVCYMWWDIIPIYGMPEDPDRQQLDHA
                     ILQVMDSTLQLDSIACRESALHGLGHWQHRYPERIGEIINRFSMSHRNLPKALEIYMR
                     NAFVGYVL"
     gene            complement(6668..>6950)
                     /locus_tag="VFQ23_19495"
     CDS             complement(6668..>6950)
                     /locus_tag="VFQ23_19495"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9908844.1"
                     /translation="VKAAMPGIKADEVQIDVTGDVLTIKGEMKHEEETKGKAWHIREH
                     RWGAFERSVRLPTDVVADKAKADFDNGILTITLPKSEEVKPKTITVKAK"
BASE COUNT         1725 a         1797 c         1687 g         1741 t
ORIGIN      
        1 ttgaccgagc agagttgatt gaagtcaccg cgcgtgcatg tatagataat acgcccgata
       61 tgttctttgg tgggtaaggg tccgggcgta aaggttggga atggagtcgg cagactgcga
      121 tcagagacat tgatgcccga caaattcatc ggagtggagg gaatcaaaat acacaaggta
      181 atcgccagaa ccaccagaac caggccaagg ggcaggcgaa agctgcgcgt ggacgggctc
      241 cgccggttaa aaattttctt tttatacatg gatggctaaa ttttacatca gagttcgcct
      301 gacgattccg cccggatcgg taaggacgat ggatatcata gatgctagtc tgtcctagcc
      361 ctcttttgtt tcaccgatgc gcgcaaagaa tgataagcaa atgttgtgtg atcatctggc
      421 taatacataa gacgtcacac aacatttgct aacctgaatt cttcggaaga attaattttg
      481 acgactgatc gtaaccggct cccctttgtc acgggatact aattcgaagg ccctgatcca
      541 tcgagttggg aacagcaaag ttgtaccagg ggaaccaata ttgggtagag agtgcctcaa
      601 cgggcaagcc catcatctcc gaataactgc tccgatagcc tggttccttc cagatgactc
      661 gcagtgcaac caggatttga tcataaggcg tgatcgtaca ggtggtgcag acaacgcgaa
      721 tcggaccatt atctacgtta tagccgactc tagtgctggt ttcggcgttt aggatgatcg
      781 tgtcaacctt attgccatcc acccaaactt cgaccgtatt ggtttcattg ggactgacat
      841 tcgcgatacg aaatccctga tccatcgagt tgacgaccgc attgttatac cagggaaacc
      901 aatactcatc cgagagttgt tctttgggca aacccatcat ttcagaatag ctgctgcgga
      961 acccgggctc tttccagatg acgcgcaatg ccgccaggat ttgatcattg tccgtgtttg
     1021 tgcaggtagt gcaggtaaca cgcacagggc cattatccac gttatagccg actcgcctgc
     1081 ttgcacccgc atccaaggag aagctttcca gtagatcatt acccgcccag acctcgatgg
     1141 tattggtttc ggcagggctg acgtttgcaa tacgaaaccc ctgatccata gagtttgcta
     1201 cagcattgtt gtaccaggga aaccaatatt ccgtggaaag ttgctctttc ggtaagccca
     1261 tcatttctga gtagctaaag cgtgagccgg gctcctgcca gatcacacgg agcgctgcaa
     1321 tgattttgtc gtcggtgttg gcggggttgc aggtaacaca cacaatgtgg atcgggccat
     1381 tgttcacgct gtatccgact cgcgtgctgc cgccaccatc cagggaaata tcaccaccaa
     1441 tttgcgtggt accaagaaac acacgaatta tatgtgtggt ggtatccaca ttggcaatgc
     1501 ggaagccctg gtccatcgaa tttgtatcga gattgttgta ccaggggaac caatattcgg
     1561 tagaaagctg ctccttcggt aagcccatca tttctgagta gctggttcgt aacccaggtt
     1621 cccgccagat cacacgtaaa gcagccagag tgtttgacgt tcccgtgctc acgacctgaa
     1681 caggtccatc atccacgccc gggtagccca cccgcaaact tccgggcgtg gggatgtaat
     1741 gatcttcgat caacgcccca ccaataaaga cgtttgtatt aggcaagaca atgtcataaa
     1801 tttcgccgga ggtgaaatcg ccattccctg caccgcctcc tcccaaagga ttgccagagg
     1861 catctacgat gctatcgtta tcagaaacaa ccaggcgaag agtgccagtt ccagtacccg
     1921 tatttacggt gaccgtgcgg gtcgaaccag atccgctcac agtactgaca gaagcaccac
     1981 tgattccacc ggtgttattt agagtgaaat cgttggtacc cacaccagtc acagattcag
     2041 aaaatgtgac gttgtatcga accgtggatg cgctggtagg gttagggtcc aatcgcgtaa
     2101 tactatttac gacaggtggc gcatcattcc agctggcaca gggatcaaag acattcatca
     2161 cgatcatctc agcaaagttc caggactgac taaaaggtcc ctgatacggt ccgccattgg
     2221 cccgaccaat gccaaggaat ccttcgctcc ccacagtacc actattgtca ttatacggat
     2281 accacaggat agcgtgacgg tgaccccagc tacagcaagg accatcttcg tacatccatc
     2341 cataaatcgc gcgttcaagg ggtagcgata tactcgaacc gctcgtgaca aacacaaata
     2401 tattctctgc aacggataga aaatcgtggc aggctccgat ggccgggttg tcctcgagac
     2461 gctgccaggg cgttctgcca tcggcagtat gaccccaggt gttattgtcc agcaggtaat
     2521 cggcataata ttgtgcgacg cccatcacgt tggcttctat cccctgcaac ggagctacgc
     2581 cgcggtcaat gcgttcgcgg ttgatcaacc agagtgcttt ttgaccgtcg gtcataccat
     2641 cccattcgct ctgactggga agtgtcatca tcgggataga gcttcccaac tgggcgtttt
     2701 cgacggtccg cgcattgttg aatgctgctt gaatgtgtgc caccgtgcta gtccccgctg
     2761 accaggggat atcggcagtc gggtctggtg ggaatgaaag agcttgatct cgtgcggtag
     2821 gttgttgaag ttctccagca gacgcaacca tactctgtgc tggtaaaaca acagccgcaa
     2881 aaagaaaaat aaatacagtt tgaatcatgc gatggagctt acggtgtatt tgcataaaac
     2941 tctccggcga aatttattac ttttgctcgt gatctcataa tcattctagc aaaacgatac
     3001 acccaagaca ataggacatt cgtgagttga caattagagt tttcctgaag tgacataatc
     3061 aacataattc gggtttaaat aataagttcg cctgaatcat tggttcaggc gaacttattg
     3121 tcagcttccg gctttacata tcatctcgag cttcattgtc ttccatttct tcctgaaact
     3181 cgcgatctgt cacttcacca tctatgccga tcgactcacc gtccatcatc atgcgtgtgg
     3241 ttccatcggt tctaatcacc atgccgtcta tcagtacccg tgtaccatcg ttcatcaata
     3301 caccttcatc catcacgacc aactcaccat ttctcacgat catcattctg cccgcttcca
     3361 ttctgactga gccttcattt agattcatgc ctttgacttc ctctctgaaa ttcaaggtca
     3421 ttccctttat tgctgataac agtaattcat tctatgaacc cataaaagat acgtcaatgg
     3481 aaggaatccc ccacgtacga attagatgag gtctacaacc catcggcctt agttacattg
     3541 aaacagcaga gattacagtc acagtcagcg agtaagtctt gcaatgaaat taacctttaa
     3601 caactcaata aattcagtat gattcccgtc caatggacgt atgtgcaaca tggaccaccc
     3661 tttcgtgaaa gaggatcata cacaatgagc ttcttgctat ccaatcgagc aaccaacaca
     3721 ctacaaaccc tgagcaagca aacctggtcc aggatgctga gccttgccag gttttatgga
     3781 tggcagccca tgggtacact gccaccgcaa actcaccatc tccgcaagcc agcgtatgag
     3841 catggaacga aaaatagttg ggacggcacg tatttgagaa acgaagggca gatcgtacga
     3901 gccgaggacg cgctcctgct cgccatggcc ctggaactgt cgctggatga tatccccgat
     3961 gtaaacccgg aacgaggcgg cgcttcctca aatgcgcaag atgaaccaac gcctcttgga
     4021 attctgccct tcgaatactt tgcaggggat ggaaaacaaa acctggcaga cttcatccga
     4081 ttttgcacgt tgggtgaatt tattattttg tgaattcaaa tcgtcccccg ggggatgtaa
     4141 gtaaactgca aagttttttt gccacacgtc aagatatact tcaaaaataa ttctttctcc
     4201 tttctcgtcg tgtacggctc ggacttggcc cagttcgagc cgtacgcatt taaggagttt
     4261 tttcacctca cggctgaaca atcgagacga tcgggtaaaa agtcaatcat tcggatttgt
     4321 tccaaaggca cgttcaatgt cccctctgcg ccgacccagc aacagtctgt gcgtgttacc
     4381 gaagatgcga cgttgatatc cacaaggagc caatttgagt cctgatgcca ttgtgcaagg
     4441 acctgcacaa cttcacccgc gttcaactgc cagcgatcaa cgtattcggt gcccggacct
     4501 tctcgacata acatgtttgt ggatgctgtg aaatatggaa ggtgctcttc atggagttgc
     4561 gttggagtaa aactggtggc cagtgcaggt gtggcactgg agctgagggt tggcgtggaa
     4621 gcttcaaccg tcagggtaac agttgagaga tcgctgctta atactggaac ttcgcttgtc
     4681 agcatcaact cggaggattc agcgcgcggt gaaggcaggg cttcagcagc cgggccccga
     4741 aaaggattgg ggagcaggcc agacgccatc ccaccagcca gaccagcaca tagacaaaga
     4801 gtgagaatgc tgaaaccgct gacaccaaat aaccccatgc gtgcgttttt acttttcacg
     4861 agtgaagcag gtcctgcaag aggttgtgaa gtgtggacag cttccgcggg cgcttgctgc
     4921 tctgcggtct ttacctgtac ctctgccata tccttagccg acgtgacaac agggatttcg
     4981 ggttcgtttt caccctcatg aatagcttgc gacaactggc ttgcagtctg gtagcgcctg
     5041 tctggagatt tatccagcgt cttgcggatg atgcgttcaa acgtgggagg gagatcaggc
     5101 cgccgttcca gtaagctggg caccggttcg ataatatgcg ccatcgcctg tcccatgggc
     5161 gtatccgctt tgtagggaag ttcgccagtc agtgcttgaa acaagactac accgagcgaa
     5221 taaatatcac aacgaccatc gagttttttc tccccctggg cctgttcggg actcatataa
     5281 gccggtgttc caataatgcc cgtccccgta attgccgccg tactttcaac gatcttggag
     5341 attccaaagt ctgaaagata actgacatgt tgtgagtcga agaggatatt tcccggcttg
     5401 atgtcacgat ggatgacacc catatggtgg gcatgatcaa gcgccgaacc gatccgttcg
     5461 aagagcgccg cgatttcagg gattggcaac gggccctccg tcagccggtc cgccagcgtc
     5521 ccgccaggca tgtaacgcat cacaatgaac ggctggtctt ccacttcccc aaagtcatag
     5581 acgggaacga tcgcgggatg ttccagcgcg gcgatcacct ctgcctcatg ctgaaagcgt
     5641 gtgcggaatt gagggtcgaa cgtaaattga cgcggtagaa ccttgattgc aacctggcgc
     5701 ttgatgtagg gatcacgtgc caggtaaacc attgccattc cgccctgacc caactcttgt
     5761 atcacttcat aacggcctat attcatagga cccgctccga tctgtctgtt tccgcacttg
     5821 gttgcttggc aggattatat cattggcaaa agctgtcact ttgcctgtgg agcgatgata
     5881 ttgaattgaa actatttttg ttctatactg gaggatatgg gcagacagct gacgaatctt
     5941 acttttgaag aatggctgac cttcgtcttt gaccacccgg ttccgaccgg gcaggaaaaa
     6001 gcatggtatt gggaattcga tgcagatgag tgggacgaaa cccatgcgga tgtcattcaa
     6061 tttcttacac aggccttcga aaatgcagag gtagtattcc agccttacac ggacgcccag
     6121 ctcaatcagg gactttggta tatcgccgat aactcatgtt ccaaccacat gtttgccctg
     6181 ctcgatacga acattccctg gaacgagcgt cagcgctgtg ttgattcaat tcaccagctt
     6241 tacgagcagt gcttcgccaa acgttgcagt ccgcatctga gtcacatcga tgaacccggg
     6301 gctaatccat tgaatgccgt ttgctatatg tggtgggata ttattccgat ctatggcatg
     6361 ccggaagacc ctgatcggca acagctcgat cacgctattc ttcaagtaat ggattcgaca
     6421 ctgcagcttg actcaatcgc ctgtcgcgaa agtgcgctac acgggctggg tcattggcag
     6481 caccggtatc cggaacgcat tggagagatt atcaacagat tctccatgtc ccaccgaaat
     6541 ttgccgaaag ccctggaaat ttacatgcgc aatgcctttg tcggatatgt actctagctc
     6601 cgcgccttaa atttatctgg aatcaaagaa ggcgcggcac tttgtgcccc gccttcctgt
     6661 tagtttgtta cttggccttg acggtgatcg tcttcggttt cacttcttct gatttcggca
     6721 gagtgatggt caaaatacca ttgtcgaaat cagcttttgc cttgtcagcg accacatccg
     6781 tcggaagccg tacggagcgt tcgaaggcac cccagcgatg ctcacgaatg tgccaggctt
     6841 tccccttcgt ttcctcttcg tgcttcatct cacctttgat ggtaagaaca tctccggtca
     6901 cgtcgatctg gacctcatcg gctttgatcc ccggcatggc agctttcaca
//
LOCUS       DASTJP010000668         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1534798, whole genome shotgun sequence.
ACCESSION   DASTJP010000668 DASTJP010000000
VERSION     DASTJP010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534798"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFQ23_19500"
     CDS             <1..565
                     /locus_tag="VFQ23_19500"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908845.1"
                     /translation="QVTLGGDRVSILLVDREKKFLDVRAAVGYANEVYEMRIPIGDGV
                     TGWVAAHRRTLRINNVLQDTRYIEGSPNTRSEIAIPLLYRSELLGILNVESEQLSAYA
                     ESDEELLGTLGGSLAAIIANARLLEQIRAQAERERVLFEITDKIRRTTDMETILTTTV
                     SELTRVVGANRAKIKVGVNLPNANQKK"
     gene            573..2522
                     /locus_tag="VFQ23_19505"
     CDS             573..2522
                     /locus_tag="VFQ23_19505"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908846.1"
                     /translation="MSKVFSLDRLLARTGDRYIISVIAVAQLIAILGAIPGLLSINAN
                     AEFTPQQMQLFSWVMPVSIVLTYLVLLGISWGITPTARKRLSKWLQGSHSSTTEEEYK
                     AWREITSLTWRYGMAAGFVIFFINIIPAYLITWSQSEVISSVFQPTSINSPVPAYVLL
                     GGTVSLLGFVILSVLMIERLILPARLVLVPTEFDTQLEGRFGALLIGKFQILILGLIA
                     ISILLIAPIGYQQTVRILYSEVSSLESFSNLQVQLILFSSMALLLGAGFSYYVSKSLS
                     DPIRELINTFLKIEQGDLATRAPVNATDELGIVTMHFNRMVAQLEGLQNTLEQKVAER
                     TKQLTATNEVGRVAASILDPDELLNRVINLLSERFGYYFAAIYLLDPSEKWAELRDAT
                     GDAGHVLKNNHHRLEVAGRSMVGTAIRERSARIAQASSSEKQRFENPLLPYTRSEIAL
                     PLLAGDRVLGALDVQSTKESDFGPELIETMENMAAQVAIALENARLYQEAQRVIKEMR
                     TVQQQYLQEGWSGYSAQHDELEYGVGDDVLENSTQMEVPISLRDQILGQIVLEGNSEW
                     TQDQRSLVDAVATQAAIALENARLVSESRQVAVRERMLAEINSKIWSSATIDGILQTA
                     AKELGRRMNASQVTVELKLDDDANS"
     gene            2552..>3098
                     /locus_tag="VFQ23_19510"
     CDS             2552..>3098
                     /locus_tag="VFQ23_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908847.1"
                     /translation="MDTNKYPSLRRRLAIVLIILAALFSAGVTSILYFNFQTEFRDSL
                     RHRLENITSLAGLQQNGDVFVQVQAAHDEAFEIIHEKNVKIKRTDPELRFVYTMRKDE
                     QGIYFVVDAQVSPDEPDISEFGDRYEEPSATLVNNFDTMTGTLIEPEFYTDEFGTFLS
                     GYAPIFTSNGERVGVLGVDIAA"
BASE COUNT          827 a          728 c          774 g          769 t
ORIGIN      
        1 acaggtcacc ctgggcggag atcgggtttc catcctgctc gtcgatcgag aaaaaaaatt
       61 tctggatgta agagccgcag taggatacgc aaatgaagtc tacgaaatgc ggattcccat
      121 tggggatgga gtcactggct gggtggcggc gcatcgtcga actctgcgca tcaataatgt
      181 gcttcaggat acccgctaca ttgaaggaag ccccaacact cgttcagaaa tagcgatccc
      241 acttttatat cgaagcgaac tactgggaat ccttaacgtt gaaagcgagc aactgtcagc
      301 gtatgcggag agtgacgagg aattgttagg cacgttgggc ggtagtcttg ctgcaatcat
      361 tgcgaatgcc cgcctgctgg aacagattcg agctcaggcg gagcgagagc gagttttatt
      421 cgagataacc gacaaaattc gccggacgac agatatggaa acaattctga caacaaccgt
      481 gagtgaattg actcgcgtcg ttggtgccaa ccgagcaaaa atcaaggtgg gagtaaatct
      541 gccgaatgca aatcagaaga agtgaacagt caatgagtaa agtattctca cttgatcgat
      601 tactggctcg aaccggcgac cgatacatca tttcagtcat agccgttgct cagctgatcg
      661 caatactcgg agcgatcccg ggacttttat cgataaacgc gaacgccgaa ttcaccccac
      721 agcagatgca gcttttttca tgggtaatgc ccgtatccat tgtcttaact tacctggttc
      781 ttctgggtat cagctggggc attacgccca cagctcgcaa aagattaagc aaatggctgc
      841 aaggctcgca cagctctact acggaagaag aatataaggc atggcgcgag attacaagtc
      901 tcacctggcg ttacggaatg gcggccgggt ttgttatttt cttcattaat attattccgg
      961 cttatttaat tacctggtca caaagtgaag tgatctcttc agttttccaa cccacatcta
     1021 ttaattcgcc ggttccagca tatgtgctgc ttggtggtac cgtttcttta cttgggtttg
     1081 taattctttc ggtccttatg attgaaaggc tcatcctgcc ggcccgtctc gtgctggttc
     1141 ccactgaatt cgatacgcaa ctggagggac ggtttggcgc cctattaatc gggaagtttc
     1201 aaattctaat tctgggtttg atcgccatca gtattttgtt gatcgccccg atcggttatc
     1261 agcaaaccgt tcgcattctt tactcagagg tcagctcgct ggaaagcttt agtaatcttc
     1321 aggttcaatt gattctattc agcagcatgg cactactctt gggagcaggc ttttcatact
     1381 atgtttccaa gtctctatca gaccccatta gagaactaat caacacattt ctcaaaatcg
     1441 agcagggaga tcttgccacg cgggccccgg taaacgcaac agacgaattg gggatcgtca
     1501 ccatgcattt caatcgcatg gtcgcacaac tcgaaggact tcaaaacacc cttgagcaaa
     1561 aggttgcaga acgcacgaag cagctcactg caacaaatga ggtcgggaga gttgccgcct
     1621 ccattctcga ccccgacgaa ttacttaacc gagttattaa tttgctttcg gagagatttg
     1681 gctattactt tgctgcaatt tatttgttag atcctagtga aaaatgggca gaacttcggg
     1741 atgccaccgg agacgccgga cacgtactca agaacaatca tcaccgcctt gaagtggcgg
     1801 gaagaagcat ggtcggcacg gccatccgtg aaaggtcggc tcgcatcgca caagcgtcct
     1861 cctccgaaaa acaacggttt gagaatccat tgcttcctta tacaagatcc gaaatcgcgc
     1921 tgccgttgtt ggctggggat cgtgtgcttg gcgctttgga tgttcaatcc accaaagagt
     1981 ctgattttgg cccggagctc atcgaaacga tggaaaacat ggctgctcag gtagctattg
     2041 ctctggaaaa cgcacgcctg taccaggaag cgcagcgtgt aattaaagaa atgcgaacgg
     2101 tccagcagca atatctgcag gaaggctgga gtggatattc ggctcagcat gatgagcttg
     2161 aatatggcgt tggtgacgat gttctcgaaa attccacaca aatggaagtt cccatcagcc
     2221 tgcgcgatca aatccttggt caaatcgtgc tcgagggaaa cagcgagtgg acccaggatc
     2281 aacgcagcct ggtggatgca gtggcgacac aagccgccat tgccctggaa aatgcgcgtt
     2341 tagtaagcga gagccgacaa gtggcagtcc gcgaacgtat gctggcagaa atcaattcca
     2401 agatctggtc ttcagcaaca atcgatggaa tactgcaaac cgccgcgaaa gaactggggc
     2461 ggcggatgaa tgcatctcaa gttacagttg aattgaagct agatgatgat gcaaactcgt
     2521 aagcgtcgga tcagcttgct tcatgaacaa tatggataca aataagtacc cgtctcttcg
     2581 aagaagactt gccatagtgt tgatcatcct tgcggcatta ttttccgcgg gtgtcacttc
     2641 tattctttat ttcaatttcc aaaccgaatt tagagatagt ttgcgtcatc ggcttgaaaa
     2701 tattacctct ctggcaggcc tgcaacaaaa cggcgatgtg tttgtacaag tccaggccgc
     2761 gcatgatgag gcgtttgaga tcattcatga gaagaatgta aagatcaagc gcaccgaccc
     2821 cgaactacga tttgtttaca cgatgaggaa agatgaacag ggaatctatt tcgtagtgga
     2881 tgcgcaagta tccccggatg agcctgacat ctctgaattc ggtgatcgat atgaggagcc
     2941 aagtgccacc ttagtgaaca actttgatac gatgacgggc acactcatcg aaccagaatt
     3001 ttacacggac gagttcggta cgtttctctc tggatatgct ccgattttca cgtcaaacgg
     3061 cgaaagagta ggcgtcttag gcgtagacat tgccgcca
//
LOCUS       DASTJP010000669         4783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1534818, whole genome shotgun sequence.
ACCESSION   DASTJP010000669 DASTJP010000000
VERSION     DASTJP010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534818"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFQ23_19515"
     CDS             <1..787
                     /locus_tag="VFQ23_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908848.1"
                     /translation="IEATPEVPPTEVPADPNRLTPDGILLEMAFEPTFFRPEASYEFG
                     RPPVFALLADGRVIYTEEGQTFDQERVMIAQLSPEEMAELQQQVLDLGFDRLETYTDF
                     CSPASSAEQVCVSDAAYTILRMRQPDDSLKEVKIYANFAEDVEAFEGITSLLSGYTHP
                     DAEPYVPTKAALFISETQGETPETVLDWPFDPALLQLPQSVTGLSAIVLEGEELSEYL
                     ALVERNTGDAFFQHEDKLYRAYFVPWLPAADYSEELLAEFPGA"
     gene            937..1443
                     /locus_tag="VFQ23_19520"
     CDS             937..1443
                     /locus_tag="VFQ23_19520"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET9908849.1"
                     /translation="MKPFVKRIVSLCMGSFILFLAACGSRSVAGSNPEEPVLPISAEI
                     TPSTGADLVNTQWALEAIVADGIESPSLSTTSPYLEFRDNEAARGSGGCNTFSTQYQA
                     QDGRISFGPVAATKIACAVEILQQEQSLFDALASADRFEISGDTLQIWYAQGQSILTF
                     SRTTISSP"
     gene            complement(1507..2262)
                     /locus_tag="VFQ23_19525"
     CDS             complement(1507..2262)
                     /locus_tag="VFQ23_19525"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9908850.1"
                     /translation="MLLLIGLSTYLFSPGRFLQTSAEQMPESSSSGILTTIPTQTEEP
                     ITLSACVTVTTNIRREPGTKYETIGGLLPGTCLTIIGRNSEASWAYIVSEDHQTGWVD
                     VSLLNDSWGIDKVSIREGSAMANPGRATLTSEEIAYGAHLYFTEVAATNIPQAPLTQY
                     MTPCFDTASRLGDHISCKIDRAVCDYLPAVEGSPTICNDLPYPNHSFALVVFGKDWSE
                     YDRECIIVSGYLKIDKGVLQIQADQRAQVSPCK"
     gene            complement(2303..2452)
                     /locus_tag="VFQ23_19530"
     CDS             complement(2303..2452)
                     /locus_tag="VFQ23_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908851.1"
                     /translation="MLQFDYPIPILKKETSSNDEDMYSLHNGKPRRGNFLQAMQARPA
                     GTANA"
     gene            complement(2671..2946)
                     /locus_tag="VFQ23_19535"
     CDS             complement(2671..2946)
                     /locus_tag="VFQ23_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908852.1"
                     /translation="MSNNNFIRPGKHGSQVTLDPGSLLSHQLYMPDGETIVRSNMTLG
                     QAIEAIAFAHVKLKMPHLADKCNAAKIEFADGKFVVCFADSIDNLSF"
     gene            complement(2915..3319)
                     /locus_tag="VFQ23_19540"
     CDS             complement(2915..3319)
                     /locus_tag="VFQ23_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908853.1"
                     /translation="MCIYVWKFNTIPDEKVVERFIDDQMPLFARNASDLTGGDGEPVV
                     DLMKVEHNCGAVNLEFQLNGVEDESGDVFCFPGYYPGDMDDDGNLLFNLCNTKGHYYG
                     QVVETVLSRAQKLFDMEVMSYVSEQQQFHSAW"
     gene            complement(3483..3866)
                     /locus_tag="VFQ23_19545"
     CDS             complement(3483..3866)
                     /locus_tag="VFQ23_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908854.1"
                     /translation="MYYHLSSQSTVAREKFSKDSWPKVLSLAMAYGWKPIGTYPAPRI
                     EIWGLDPEDWNGTYLTNDGQIVIAEDASSLAIALEKALDDIPDFIIEEGPTEHPFEHF
                     AGEEKSHLVDFIKFCQLGSFTILRI"
     gene            4006..>4783
                     /locus_tag="VFQ23_19550"
     CDS             4006..>4783
                     /locus_tag="VFQ23_19550"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9908855.1"
                     /translation="MDTVIKNINLIDGTGRDPVQDAILEIRAGKISYAGPASGWSAAS
                     SGDSIDLDLDGQYVLPGLIDCHVHLSGSGEADSQFSAPTGQMALKILKNAQRNLAAGI
                     TTVRDLGGWSELEFDVRRAIKRDEFAGPRMCLAGRFISITEAGAEYYLGMYRVADGVD
                     EVRKAVREQVKNGADVIKMGVTGAVLVESGVPGTTHFNADEVCALVEEARKFSKRVAA
                     HAHGIDGIRKAVQAGINTLEHGTYLYQDPLVIQQMAEQRVF"
BASE COUNT         1160 a         1186 c         1210 g         1227 t
ORIGIN      
        1 tatcgaagct actcccgaag taccgccaac ggaggtcccg gcggatccaa accggctgac
       61 gcctgatgga attttgctgg aaatggcctt cgagcccacc ttcttccgcc ccgaggcctc
      121 gtatgaattt ggccgtcctc ctgtcttcgc cttgctggca gatgggcgcg taatttatac
      181 cgaggaaggt caaaccttcg accaggagcg ggtaatgatt gcgcagctct ctccggagga
      241 aatggctgaa ctgcagcagc aagtgttaga tctcggtttt gatcgtctgg agacctatac
      301 ggatttctgt tctccggcgt ccagtgctga acaggtttgc gtgtcggatg ccgcttacac
      361 aattctccgt atgcgccagc cggatgactc gctcaaagaa gtgaagatct atgccaattt
      421 tgctgaggat gtggaagcat tcgaaggcat tacaagcctg ctgagcggct acactcatcc
      481 ggatgccgag ccctatgttc caacgaaggc cgcgctgttc atttctgaaa ctcagggcga
      541 gacacctgaa accgttctcg attggccctt cgacccggct ctccttcaac tccctcaaag
      601 tgtcactgga ctgtcagcca tcgtgctgga gggtgaggaa ctgagcgaat acctcgcact
      661 tgtggaacgc aacactggcg atgccttctt ccagcatgag gacaagctct atcgggccta
      721 ttttgttccc tggctgccgg ccgccgatta cagtgaagaa ttactggctg agttcccagg
      781 agcataggtt tactcctcac gaagcccacg acccttcgct cctttaggag cgaagggtcg
      841 tgatcgtttc ttgccagtgg actggcaaca acgatttttt gtggtagtct tttggtggtt
      901 tctcccaact tgagtataca gataaggagg tagaaaatga aaccgtttgt gaaacgaatc
      961 gtttctcttt gtatggggtc gttcatcctg tttctggctg cctgcggatc gaggtcggta
     1021 gcaggctcca accctgaaga gccggtcctc cccataagtg cagaaatcac accgagtacc
     1081 ggagcagatt tggtcaatac ccaatgggca ctcgaggcga ttgtcgcaga cggaatcgaa
     1141 tctccgtccc tatcaacgac ttctccgtat ttggaatttc gtgacaacga agccgcgcga
     1201 ggttcaggag gatgcaacac ctttagcacc caatatcagg cacaggatgg aaggatttct
     1261 ttcggaccag tcgccgccac aaagatcgcc tgtgcggtgg aaattctgca gcaggaacaa
     1321 tcgcttttcg atgcgctggc gtcagcagac cgatttgaga tatccggtga cactctgcag
     1381 atctggtacg cacaagggca aagtattctt acgttttcaa ggactacaat cagttcgcct
     1441 taatcctgat gagatcatcc gtaccctaat gcctgaagta ggaagcccag accaacacat
     1501 gcgcacttac ttacaagggg acacctgcgc gcgttgatcg gcctggatct gcagcacacc
     1561 tttatctatt ttgagatagc ccgaaacgat aatacattcg cgatcgtatt cgctccaatc
     1621 cttaccaaat acaactaatg caaagctatg gttcgggtag ggtagatcat tgcaaattgt
     1681 cgggctgcct tcgactgcgg gcagatagtc gcaaactgcc ctgtcgattt tacaggagat
     1741 gtgatcacca agtcggctgg cggtatcaaa gcatggagtc atatattgtg tgagaggggc
     1801 ttgaggaatg ttggtcgccg caacttccgt gaagtaaagg tgtgctccat aagcgatttc
     1861 ttcactggtg agcgttgccc tgccggggtt tgccatggca gaaccttccc gtattgagac
     1921 tttatcgatc ccccaagaat catttagcag tgaaacatcc acccaacccg tctgatgatc
     1981 ctcgctgacg atgtaggccc aacttgcttc cgaatttcgt ccgatgattg tcagacacgt
     2041 acctgggagc agtccgccaa ttgtttcata ctttgtgcca ggttcacgtc tgatgttggt
     2101 tgtgaccgtt acgcatgcgc tgagcgtgat gggttcttct gtttgggttg gtatagtagt
     2161 taggatcccg cttgaactcg actccggcat ttgttcagca ctggtttgta gaaatctgcc
     2221 tggcgaaaac agataagtgc tcagtccgat caaaagtagc attcccagaa accaacggcg
     2281 gaaggaatgt gaagcccgtt cttcaggcgt tcgcggtgcc tgcagggcgt gcctgcattg
     2341 cctgcagaaa attgcctcgt cgcggttttc cgttgtgcag tgagtacata tcttcatcgt
     2401 tcgaagatgt ttcctttttc aatatgggaa tgggataatc aaattgtagc attacattct
     2461 aagaaaatga tgttaattcg ggtcagtatt aattaaatgc gagaaccgcg gcgccgaccg
     2521 cggttctctt aaggaggaga agagaaatcc cctggttgtt caaatgtagc atacccatct
     2581 caaccgtgct tgactctctg cctatgattg cccgatgata tcccgacggg agaggcattc
     2641 attgcgtatt gaaagacgga aattacttta tcagaaactt aagttatcga tgctgtctgc
     2701 aaagcacacc acgaacttgc catcggcgaa ttcgatcttt gccgcgttgc atttatcggc
     2761 cagatgtggc atcttcaatt tgacatgcgc gaacgcgatc gcttcgatcg cctgtccaag
     2821 cgtcatattc gacctgacga ttgtctctcc gtctggcata taaagctgat gactcaggag
     2881 agagcccggg tcaagcgtga cctgtgatcc gtgcttacca ggccgaatga aattgttgtt
     2941 gctcactgac ataactcatt acctccatgt caaataactt ctgcgcgcga ctcaagacgg
     3001 tttctaccac ctgtccataa tagtgacctt ttgtgttaca taggttgaaa agaagattgc
     3061 catcatcgtc catgtcaccg gggtaatagc ctgggaaaca aaacacgtcc ccgctttcgt
     3121 cctcgacacc gttaagctgg aactccaaat taacagcccc gcaattatgt tcgactttca
     3181 tcaggtcgac gacaggctca ccatctcccc cggtcaggtc gctggcattg cgcgcgaaca
     3241 aaggcatttg atcatcgata aatcgttcaa caaccttctc gtcaggaatg gtgttgaact
     3301 tccacacata aatacacata agacttcctt tttatttcgt gtaaaggaca catataaggt
     3361 ttcaggtcaa gctatgtatt tagaacactt gttctaaaat ttgacacaag gttaccacaa
     3421 aaatatgaga tatggaagag gcaattttag ttaggctgaa tgatgaagcg tagcaactgg
     3481 cttcagatgc gcaggatggt aaaacttccc aactggcaga atttgatgaa atcaaccagg
     3541 tgactttttt cttcccccgc aaaatgttcg aaaggatgct ctgtcggtcc ctcttcgatg
     3601 atgaaatcgg ggatatcatc cagagccttc tccagagcaa tcgcaagcga tgacgcatct
     3661 tccgcgatca cgatctgacc atcgttcgtg agataggtcc cattccaatc ttcgggatcc
     3721 agtccccaga tctcgatccg tggagccggg tatgttccga tcggtttcca gccataagcc
     3781 attgccaggc tcaagacctt gggccaggaa tccttgctga atttttctct tgcaacggtg
     3841 gattggctgg agagatggta atacataatg cttatcataa ttatactcag ccttttggtt
     3901 cagaataata acactatcct tagtctcatc atttactaaa ctcttctcat aattggagat
     3961 aaaattgatt tactcaattt caaaactctt caaaatggat ttcaaatgga taccgttata
     4021 aaaaacatca atctcatcga cgggacgggg cgtgatccag ttcaagacgc gatcctggag
     4081 attcgcgctg gaaaaatctc ctacgcggga cctgcctcag gctggtctgc ggcaagttca
     4141 ggcgattcaa tcgatcttga cctggatggt caatatgttc tccccgggct gatcgattgc
     4201 cacgtccact tatctggctc gggtgaagca gacagtcagt tctctgctcc gaccgggcaa
     4261 atggcattga agattttgaa gaacgcccag cggaatctgg ctgcggggat taccaccgtc
     4321 cgtgacctgg gtgggtggag tgagcttgaa ttcgatgtgc ggcgcgccat caaacgcgat
     4381 gagtttgcag gtccacgcat gtgtctggcg ggccggttta tctccatcac ggaagcgggt
     4441 gctgaatatt atctcggcat gtatcgcgtt gctgatggtg tggatgaggt ccgcaaggct
     4501 gtgcgtgaac aggtgaagaa cggggcagac gtaatcaaga tgggcgtgac cggcgccgtg
     4561 ctggttgaaa gtggtgtgcc tggcaccaca cacttcaatg cagatgaagt ctgtgcgctg
     4621 gtggaggaag ccaggaaatt tagcaagcgt gtggctgctc acgcgcatgg catcgacggc
     4681 atcaggaagg ctgtgcaagc tggtatcaac acgctcgaac atggaacgta tctctatcag
     4741 gacccgctcg tgattcaaca gatggcagag cagagagtgt ttc
//
LOCUS       DASTJP010000670         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_584287, whole genome shotgun sequence.
ACCESSION   DASTJP010000670 DASTJP010000000
VERSION     DASTJP010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584287"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VFQ23_19555"
     CDS             complement(<1..1300)
                     /locus_tag="VFQ23_19555"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET9908856.1"
                     /translation="MILTRKLIFPALIIFAVLLAGIFAYSYFSLHDTYHEAEEADLVS
                     YTDAFLAEIENQKNVALALASTAAGNPEIQKAFSQRNRQQLASLTLPGFETLKSYNVT
                     QYQYYLPDGQRFLSLNDINDQRTGQTLSVVLLSNSEERAVAGLETENGMLGVRGAVPI
                     YDQGIHIGAVEVGIEFNTASLDSLKEKYGGEWHILLSKDLAVDPSSASAPIPELIPYA
                     TTQSKVLPNDSSSYTMALEGSTSITHPSVDGRDYAILTSPLRDFSGRIVGTLDIVYDH
                     THISDTQNRRLLLTALVTILALVLGSVSLIILTTRTLQPIQTLTSTAAAIAEGNITRP
                     IDSRPGEDEIATLTTAFNRMTTQLRNSITDLEKRVTDRTQDLEKQTKWLRLAAEVVRD
                     ATYTRNLEEMLNRSTGLILDRFKFYHTGIFLLDNNNEYAVL"
     gene            complement(1319..>2773)
                     /locus_tag="VFQ23_19560"
     CDS             complement(1319..>2773)
                     /locus_tag="VFQ23_19560"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908857.1"
                     /translation="FDTMTGTIIVPDFYTDEFGTFLSGYTPIFTSDGERVGVLGVDIA
                     ANTILARELEFRNRLMVIFSVALILIVIVGYFSADYLARPIVGLRDAAKKISSGDFAY
                     RISKIPTTRELAELAIDFNKMTENLSGLINDLEQRVAARTADIERKTDQLRAAAFIAR
                     QTAEVHDLESLLDIVVRLVSDRFGFYHTGIFLINEAGDEALLQAASSEGGLRMIEKGH
                     AIGVGSLSTVGYVLSQKKSRIVFDVGSDAVLFNNPDLPMTRSQAALPLVVRNKILGVL
                     DIHSDTPQAFGGEDIDVLQTLADQVAVAIENARLLDESQAAVTQLETLTADRTGEAWS
                     QELQNRGRAFTYTPLGLRAERASIKDNNTINTTISLRGYKVGNISISRKEDVAWNKSD
                     EDFVLEVANQIGLAVDNIRLLEDATQRAKQEQTVGRLAARFGQSLDIDTLLQTAAREL
                     GQLPDVEEATVFISETEPDTKITANTTTKKTRVA"
BASE COUNT          703 a          671 c          655 g          744 t
ORIGIN      
        1 ccagtaccgc gtactcatta ttgttgtcca gaagaaatat tccggtgtga taaaacttga
       61 accgatccag aatcagccct gtggaacgat tcagcatctc ctccaggttt ctggtgtacg
      121 tggcatcccg tactacttca gccgccaggc gcaaccattt ggtttgtttc tctaaatcct
      181 gcgtgcggtc agtaacgcgt ttttccagat ccgtaatcga attgcgtaac tgcgttgtca
      241 ttcgattgaa cgccgtagtt aacgttgcga tttcatcttc cccgggtcta ctatcgattg
      301 gtcgtgtgat attgccttcc gcaatggcgg cggcagtact cgtgagtgtt tgaatcggtt
      361 gcagtgtgcg cgttgtgaga atgatcaggc ttacacttcc caggaccagt gcaagaatgg
      421 tgaccagagc tgtaagcaga agtcggcgat tctgagtatc cgagatgtgg gtatggtcgt
      481 agacaatatc cagggtgccg acaattcgcc cggagaaatc ccgcagtggg gaagtcaaaa
      541 ttgcataatc tctcccatcg acactagggt gggtaatgga ggttgatcct tccagagcca
      601 tagtgtaact ggagctgtcg ttaggaagga ctttactttg agtagtcgca tatgggatta
      661 gttcgggaat gggcgccgat gccgaggatg ggtcaacagc tagatccttg gagaggagaa
      721 tatgccactc tccaccatat ttctctttga gactgtctaa cgaggcagta ttaaattcga
      781 ttccgacttc cacggctcca atgtgaattc cctgatcgta aatgggaact gcgccccgca
      841 cacccagcat gccattttca gtttctaagc ctgccactgc cctttcctca gagtttgata
      901 ataacacgac cgataatgtt tggccagtcc tttgatcgtt gatgtcattc aagctgagga
      961 atcgctgtcc gtctggaaga taatattgat actgagtgac gttgtaagat ttcagcgttt
     1021 caaaaccagg cagcgttaat gaagccagct gttggcggtt ccgttgtgaa aaagcctttt
     1081 gaatttccgg gttgcccgcg gctgttgatg ccagtgccag agccacattc ttttgatttt
     1141 ctatttccgc aagaaacgca tccgtatatg aaaccagatc ggcttcttcc gcttcgtgat
     1201 aggtatcgtg aagactaaaa taactatagg cgaaaatgcc tgccagcaaa actgcaaaaa
     1261 tgatcagagc gggaaaaatc aatttacgcg taagaatcat aataaataaa gccttggact
     1321 atgccaccct cgtttttttg gtggtcgtat tggctgtgat tttagtgtcc ggttcagttt
     1381 cgctgataaa aacggttgcc tcttcaacat ccggcaattg accaagttca cgtgctgctg
     1441 tttgaagcag agtgtctata tccagagatt gaccaaatcg cgcggccagt ctgcctacgg
     1501 tctgttcctg ctttgcacgt tgtgttgcat cttcaaggag acgaatgttg tcaactgcaa
     1561 gaccaatctg atttgccact tccaaaacaa agtcttcatc tgatttgttc catgcaacat
     1621 cttctttgcg tgaaattgaa atattaccga ctttataccc tcgaagcgaa atagtcgtgt
     1681 tgatcgtatt attgtctttt atcgatgccc tttcggcgcg cagtcctagc ggtgtatagg
     1741 tgaaagctcg tcctctattc tgtaactcct gactccacgc ctcgccggta cggtcggcgg
     1801 ttaaggtttc gagttgtgtg actgcggctt gagactcatc caatagacgc gcgttttcaa
     1861 tcgccacggc gacctgatct gccaacgtct gtaaaacgtc aatatcctcg ccgccgaatg
     1921 cttgcggcgt atcggagtga atatccagga cacccaggat tttgttgcga accacaagag
     1981 gcagagctgc ctgggagcga gtcatcggaa ggtccggatt attgaataaa acagcatctg
     2041 agccgacgtc aaaaacgata cgcgactttt tctgtgaaag tacataccca accgtgctta
     2101 gcgacccaac cccaattgcg tgtccttttt ctatcatgcg taagcctcct tcagaggagg
     2161 cagcctgtaa gagagcttca tcacctgctt cattaatcag gaaaataccg gtatgataaa
     2221 acccaaagcg atcactgact aagcgcacaa caatatctaa aagcgattcc aaatcatgca
     2281 cttcggccgt ttgacgggcg atgaaagcgg cagcacgcaa ctgatcagtt ttccgttcaa
     2341 tatccgcagt tcgcgcggcg acccgctgtt ccaggtcatt aataaggccg cttaggttct
     2401 cggtcatttt attaaaatca atagcaagct ctgccagttc ccgcgtagtc ggtattttgg
     2461 aaattctgta cgcaaagtct ccactgctga tttttttcgc tgcatcgcgc agaccaacga
     2521 tcggtcgggc aagataatcg gcggaaaaat agccgacaat tacaatcaag atcagggcaa
     2581 cactaaaaat gaccatcagc ctgttgcgaa actccaactc cctcgccaaa atcgtgttgg
     2641 cggcaatgtc tacacctaag acgcctactc tttcgccatc cgaagtgaaa ataggagtat
     2701 agccagacag gaaagtaccg aactcgtctg tgtaaaaatc tggtacgatg attgtgcccg
     2761 tcatcgtatc aaa
//
LOCUS       DASTJP010000671         7498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1052509, whole genome shotgun sequence.
ACCESSION   DASTJP010000671 DASTJP010000000
VERSION     DASTJP010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052509"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFQ23_19565"
     CDS             complement(<1..548)
                     /locus_tag="VFQ23_19565"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908858.1"
                     /translation="METTLDPERQKQAKQYARIRRRLWLVDTIFGAVYALAWLFFGWA
                     TALRNWLATFTSNEWLLVALFVAVFGGIYSIITLPLGYYSGFVLPHRFGQSNQTFKEW
                     VVDQLKGLAVGAPLSLILLELLYLALRVTDDLWWLWAAAGMLIFTVLLSNLAPILIAP
                     IFNKYVPLGDEHKELADRLLALA"
     gene            587..1966
                     /locus_tag="VFQ23_19570"
     CDS             587..1966
                     /locus_tag="VFQ23_19570"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9908859.1"
                     /translation="MNATHHKLTGMTGFTIVWAGQLISVLASSMTQFAITIWAYQETG
                     SATALGIINTAFIVPFLLLSPVAGVMVDRHNRKLMMMVSDLTAVLATTGILILRLSGN
                     LEIWHLYIAAVINGLGNTFQWPAYSAAISTMVPKENYSRANGMMSLVESVPGVLAPIL
                     AGILLLSIELTGILIIDLVTFVIAISVLLLVHIPQPPRTAEGEAERGSILKEAAYGFK
                     YIFARRGLLGLLIFFIVLNFVIGLSGSLFSPFILERTNQNSAILGVVTSANAIGAVIG
                     GLLISLWGGFKRRMTSIFIGEALTGLFLLTIFGLGRSLPIWVISVVIGGIFPIFTNGA
                     SQAIWQAKVAPDLQGRVFSARRMIAWSVGPITPILAGLLADYVTEPAMQSQTWLARAF
                     GWMVGNESGSGMALQFVITGIAYMLVALIVYLFFPHVRNLEDELPDHDQIPKADEGST
                     SSVSASGSA"
     gene            complement(1963..2532)
                     /locus_tag="VFQ23_19575"
     CDS             complement(1963..2532)
                     /locus_tag="VFQ23_19575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HET9908860.1"
                     /translation="MKTALLIIDIQNDYFPGGKYPLVNPLDAAQNAYMLLQCFRDVGG
                     HHVHIQHISLEPDAKFFIKGDSGSDIHDSVAHFEGEPIVYKHEVNSFLDTNLLELLQG
                     WEVERLIITGMMTHMCVDGTARAASDLGFQVIVAEDACATRDLQYGDTTVPAEHVHKA
                     FLAALKAYGRVMKSDEILALLAAEQAKAS"
     gene            complement(2654..3289)
                     /locus_tag="VFQ23_19580"
     CDS             complement(2654..3289)
                     /locus_tag="VFQ23_19580"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9908861.1"
                     /translation="MNFNGTFTQVIDELVQGVMPSLVMVRGHRHGAGAGIIWDANGLI
                     LTNNHVVGRRTPIVILQDDGEYESKLIARDPDVDLALLSIDAINLTPLKPVSREASPR
                     VGEMVFAFGHPWGQHNTVTRGIISALTFAQNRRGDKLPIVRSDVPLAPGNSGGPLVNA
                     SGEVVGINAMIVGGDQSVSIAASVASDFVRKALANNDVKGKSSQRTPNDVM"
     gene            complement(3354..4253)
                     /locus_tag="VFQ23_19585"
     CDS             complement(3354..4253)
                     /locus_tag="VFQ23_19585"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9908862.1"
                     /translation="MTKNVLVELSDALADAAETAGKATVLVNARRRMPASGIAYAADL
                     ILTADHVIEREEDIKVTLADGTEVSAQIAGRDAGTDLAVLKLQQGAATVAETTKSPAR
                     LGQIALVLGRPSANGIEASLGTVSAIGGPIRTGRGGMLEKYLRTDGISYPGFSGGPLV
                     AADGTVLGLNTSGLASGAAVTIPADIAWRVAETLVKHGRIKRGYLGIRSQAVEIPSAS
                     QQALNREQGAGLLVVSVEKDSPASKGGLIVGDILVGVAGTPVFHHDELFARLNGDVVG
                     KTTPIEILRGGQPQTINVVIGER"
     gene            complement(4412..5518)
                     /locus_tag="VFQ23_19590"
     CDS             complement(4412..5518)
                     /locus_tag="VFQ23_19590"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET9908863.1"
                     /translation="MESKKRYYDIDWLRALGMMGIFLFHNARFFNEEDWHVKNAQLDF
                     GMTVFVAILNQFIMPLFFVLSAMATYYALCHRTGGEFLGERASRLLIPLIFGIFVLVP
                     PQVYVERVSHGEFQGSFFEFISHYFEGWYGFGGNFAWMGLHLWYLLMLFLFSAIMLPV
                     FQQMNKSSLSRIADFFTRSFAVYLFFIPIAIIEMLVNLSPETVGRRDFGGWSPLTYLI
                     IFFLGYMLMTDERYRPAIERIRFISLTLSLLVMTIGFALVLAFDVSTYNPLFSWLRAF
                     NTWMWLLTFLGFASRHLNFNNNFLKYANQAVLPFYILHQTVIVIIGFLIANWTLAVFP
                     KYLFLAITSFLVIMILYEFLVKRINVLRFLFGMK"
     gene            complement(5522..6145)
                     /locus_tag="VFQ23_19595"
     CDS             complement(5522..6145)
                     /locus_tag="VFQ23_19595"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HET9908864.1"
                     /translation="MPKQKYYVVWKGRKPGIFTAWAEAEKQVKGFAGAQYKAFDSLKE
                     AEAAYRSQYEEFKGKPASHGKWREASVKPILPSVCVDAACSGSPGKLEYRGVFTELET
                     EIFHFGPFPDGTNNVGEFLAVVHALTWMSKNESPLPVYSDSQNAISWVYTGKCRTKLK
                     HTTKNAPLFVMIRSAENWLAENELLDDAVLKWDTELWGEIPADFGRK"
     gene            complement(6230..6640)
                     /locus_tag="VFQ23_19600"
     CDS             complement(6230..6640)
                     /locus_tag="VFQ23_19600"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9908865.1"
                     /translation="MPNTVRDWMSSPVVVVDPDSSVKYALTLMRRRKIHSVVVASSEK
                     NPTYGIVTSTDIRDKIVAANRNPSETTVSEIMSGPIVTGNPNWTLMECSQVMQEKHIH
                     HLPIADESGSLIGLISATDIFMAVEETGWQEKEE"
     gene            complement(6691..>7498)
                     /locus_tag="VFQ23_19605"
     CDS             complement(6691..>7498)
                     /locus_tag="VFQ23_19605"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET9908866.1"
                     /translation="IGSPTPAPAFVDEIRNTQYQLGIPDSLQIVQLSDGKYEKGVSGS
                     TDFISVFVTDFIARGHLNDDGQNEVVALVSENYGGTGTFVFLAVYSEVGGDLVFQTSK
                     LVDDRPQINALLIEDQEIYLDAITHGFEDPMCCPTLKTTRHFRFIDGQLDMIDYTTFT
                     PDGRPRTITITSPASGTATYSSVQIKGEVAIAPFENNLVYRIYDVGGVELSAGAIAVA
                     APDLGLPGTFDTVIPIGTALSGAVIRIEVQDISAADGSLFAMDSVELVVQ"
BASE COUNT         1905 a         1984 c         1790 g         1819 t
ORIGIN      
        1 gccagagcaa gcaatcgatc tgcgagttcc ttgtgctcat ccccgagcgg tacatactta
       61 ttgaagatcg gtgcaatgag aatcggtgca aggtttgaaa gcagcaccgt gaagatcaac
      121 atcccggctg cggcccacag ccaccacaaa tcatcggtga cgcgcagagc tagataaagt
      181 aactcgagca ggatcaggct gaggggcgcg ccgaccgcca atcctttgag ctgatcgacc
      241 acccactcct tgaatgtctg attcgattga ccaaaacgat gcggcagaac gaatccgctg
      301 tagtagccaa gaggcaatgt gataatggaa tagatcccgc cgaagaccgc gacaaagagt
      361 gcaaccaata accattcatt actggtgaac gtcgcgagcc agttacgcag tgctgttgcc
      421 caaccgaaga atagccatgc cagcgcataa accgcgccga aaattgtatc caccagccac
      481 aaacggcgac gaatgcgcgc atactgtttg gcttgttttt gtcgttcggg gtcgagtgtc
      541 gtttccataa aaaagattat agcatgcgga gatataatcc gaactcatga atgcaaccca
      601 tcataaacta accggtatga ccgggttcac gatcgtctgg gcagggcagc taatttctgt
      661 ccttgcttcg tccatgacgc aatttgccat cacgatttgg gcttatcagg aaacgggcag
      721 cgcgaccgca ctgggaatca ttaacacggc gttcattgtc cccttcctct tactctcccc
      781 cgttgctggc gtaatggtgg accgtcacaa tcgcaaactg atgatgatgg tcagcgacct
      841 gaccgccgtc ctcgcgacaa ccgggatctt gattttgcgt ctatcgggga atttggaaat
      901 ctggcatttg tatatcgccg ccgtcattaa cggcttgggc aatacgtttc aatggccagc
      961 atattctgca gcgatcagca caatggttcc aaaagaaaac tacagccgcg caaatggcat
     1021 gatgtctttg gtagagtccg tgccgggtgt gctcgcgccg attctcgctg gtattttgtt
     1081 gctcagcata gagttgacag gtattctcat cattgacctg gtcacttttg ttatcgccat
     1141 ctctgttctt ctgctggttc atattccaca acccccgcgc accgctgaag gagaagcaga
     1201 acgtggaagt atcctcaaag aagctgccta tggatttaaa tacatcttcg cccgacgcgg
     1261 tctgcttggt ttgcttatct tctttatcgt tttaaatttc gtgattgggc tttcaggatc
     1321 gttgttttca cccttcattt tggaacgcac caatcaaaat agcgcgatac tgggtgttgt
     1381 cacctctgca aatgccatcg gcgcggtgat cggtggtttg ctgatcagtc tgtggggagg
     1441 tttcaagcgt cgcatgacca gcatcttcat cggcgaggcg ctcaccggcc tattcctgct
     1501 gacgatcttc gggctcggtc gcagcttgcc aatatgggtg atcagcgtgg tgatcggcgg
     1561 tatcttcccc atctttacca acggagccag ccaggccatc tggcaggcga aggttgcacc
     1621 cgatctacag ggaagagtct tttctgcgcg ccgcatgatc gcctggtctg tcggtccgat
     1681 tacgcccatc cttgcaggtc tgcttgcaga ttatgtcact gaacccgcca tgcaatcaca
     1741 gacatggctt gcccgagcat tcggttggat ggtaggcaat gaatctggtt cgggcatggc
     1801 attgcagttc gttattactg gcatcgccta tatgctggtt gcgttgatcg tttatctttt
     1861 ctttccccat gtgcgtaacc tggaagatga attgcctgac catgatcaaa taccaaaagc
     1921 ggacgaaggt tctacatcct ccgtttccgc ctctggttcc gcttaactgg cttttgcctg
     1981 ctctgccgcc agcagagcaa ggatttcatc gcttttcatc accctgccgt aagcctttaa
     2041 tgctgctaga aacgccttat ggacatgctc agctggaacc gtcgtatccc catattgaag
     2101 atcacgcgtc gcgcaggcat cttctgcgac gatcacctga aagcccagat ctgacgcagc
     2161 ccgggcagtt ccatccacgc acatatgcgt catcatgcct gtgatgatta ggcgctcaac
     2221 ttcccaaccc tgtaagagtt caagcaggtt cgtatcaagg aacgaattca cctcatgttt
     2281 ataaacaatg ggttcgcctt caaagtgcgc cactgagtca tggatatctg aaccgctatc
     2341 acctttgata aaaaattttg catcaggctc aagcgaaata tgttgaatat gaacgtgatg
     2401 acctccaacg tcccgaaagc attgcaacaa catatacgca ttctgtgcgg catccagagg
     2461 attcaccagt gggtacttcc caccggggaa ataatcattt tggatatcaa taataagtag
     2521 agctgtcttc acgataatat ctccaataat tcggattttg tgtacctttc acagtatagc
     2581 cgaaattaga tggctaagaa aagagaccga gtcattggtg actcggtctc ttttcaactc
     2641 taaacgtagc tcgtcacatc acatcatttg gcgtgcgttg acttgatttt cctttgacat
     2701 cattattcgc gagcgctttc ctgacaaaat cacttgccac ggaagccgcg atagaaacac
     2761 tctggtcacc accgacgatc atggcattga tcccgaccac ctccccgctt gcgttgacga
     2821 gcggtccacc cgaattgccg ggcgcgaggg gcacgtcgga acggacgatc ggcagtttat
     2881 cgccacgtcg attctgcgcg aacgtcaacg cgctgataat accgcgcgta actgtattgt
     2941 gctgtcccca cgggtgaccg aaggcgaaca ccatttcacc cacgcgcggc gacgcttcgc
     3001 gggaaacagg cttgagcgga gtcagattta tggcgtcaat agagagcaag gctaaatcta
     3061 cgtccggatc acgggcaatg agttttgatt catattcacc gtcatcctgc aaaatgacaa
     3121 tcggcgtgcg gcgtccaacc acatgattgt tggtgaggat cagtccgttt gcatcccata
     3181 taatgcccgc gcctgcacca tgccgatgac cgcgcaccat caccagcgaa ggcatcacac
     3241 cctggacaag ttcgtcaatc acttgtgtaa aagtaccatt gaagttcatg ttatttgctt
     3301 tccagacttc ggaggcgcca acgaacggcg aaacttctga agtctcatat tacctaccgt
     3361 tcaccaatga ctacattgat cgtttgaggt tgaccgccgc gcaggatttc tatcggcgtg
     3421 gtcttgccca cgacatcccc attgagccgc gcaaagagtt cgtcatgatg aaacacaggt
     3481 gttcctgcca ctccaaccag gatatcaccg acgatcaatc cgcctttgga agccgggcta
     3541 tccttttcaa cactgacgac caagagacca gccccctgct cacgattcaa cgcctgttgc
     3601 gatgcactcg gaatttcaac ggcctgactg cgaatgccga gataaccgcg cttgatacgc
     3661 ccatgcttta cgagggtctc tgcaactcgc caggcgatgt cggctggaat tgttaccgcc
     3721 gcgccactcg ccaaacctga ggtatttagc cccagcactg ttccatcggc cgccacaagc
     3781 ggaccgccag aaaagccagg atacgaaatg ccatcggttc gaaggtattt ttcaagcatg
     3841 ccaccgcgcc cggtgcggat cggtccgccg atcgcgctga ccgttccgag gctggcttcg
     3901 attccatttg cagatgggcg acccagtacc aatgcaattt gcccgaggcg cgccgggctt
     3961 ttcgtcgttt cggcaacagt cgccgcgcct tgctggagct tgaggacagc caaatctgta
     4021 ccggcatcgc gccccgcgat ctgcgctgag acttccgttc catcggcgag agtgacttta
     4081 atatcttctt cacgttcaat aacgtgatct gctgtaagga ttagatctgc cgcgtaggca
     4141 atcccgctgg cgggcatgcg gcgacgtgca tttaccagca cggtcgcttt gcctgctgtt
     4201 tcggcggcat cggcaagcgc gtcagaaagt tcgaccaata catttttcgt catggaaatt
     4261 ctcctttgta ttggtggcga gtatagtttt ggcggccctt gtgcgcatcg ccgaaaaggc
     4321 tagtttttat actggaagaa tggggaggtg gataagtaac cgccctcacc ctgccctctc
     4381 ccattgggag agggaaaggc atctggcttg attacttcat tccaaacaag aaacgtaaga
     4441 cattgatcct tttcacgagg aactcataca aaatcataat gacaaggaat gatgttattg
     4501 caagaaacaa gtacttggga aagacagcca gggtccaatt cgcaatcaag aaaccgatga
     4561 tgacaatcac cgtttgatga aggatataaa acggcaaaac ggcttgattt gcgtatttca
     4621 agaaattatt attgaagtta aggtggcgcg aggcaaagcc taaaaaggtg agcagccaca
     4681 tccaggtatt gaacgcgcgg agccaggaaa aaagcgggtt gtacgtggac acatcaaatg
     4741 ccaggaccag cgcaaagcca attgtcataa cgagcaggct caaggtcagc gaaatgaaac
     4801 ggatgcgttc aatggcagga cgatagcgtt cgtcagtcat gagcatgtat cccaaaaaga
     4861 aaattatgag atacgtaagc ggactccatc caccaaaatc acgccgtccc actgtttcag
     4921 gcgaaagatt aaccagcatt tcgatgatcg caattggaat aaaaaaaaga tagacagcaa
     4981 aagacctggt gaaaaaatca gcaatgcgag ataacgatga tttgttcatc tgctgaaata
     5041 caggcagcat aatcgctgaa aacaaaaaga gcatcaacaa ataccagaga tgcaagccca
     5101 tccaggcaaa gttgccacca aagccatacc agccttcaaa ataatgtgaa ataaattcaa
     5161 agaaggatcc ctggaattct ccatggctta cccgctcaac ataaacctgc ggtgggacca
     5221 gaacaaagat accgaagatc aatgggatta agagtcggct ggcacgctcg cccaggaatt
     5281 ctccaccggt tcgatgacac agcgcataat aagttgccat tgcggataac acaaagaaga
     5341 gcggcatgat gaactggttg aggatagcaa cgaagaccgt cataccgaaa tcgagctggg
     5401 catttttgac gtgccagtcc tcttcattga aaaagcgggc gttgtgaaat aaaaaaatcc
     5461 ccatcatccc cagagcgcgc agccagtcaa tgtcgtagta gcgttttttt gactccaact
     5521 actattttct cccgaagtcc gccgggatct ctccccacag ttcagtatcc catttcagga
     5581 ccgcatcgtc cagtaattca ttttcagcca gccaattttc cgcactgcgg atcattacaa
     5641 agagcggcgc atttttcgtc gtatgtttga gcttcgtccg acatttgcca gtataaaccc
     5701 aggaaatcgc gttttgcgaa tctgaataaa cgggtaaagg gctttcattc tttgacatcc
     5761 acgttaaagc atgcacaacc gccaggaatt cccctacatt atttgtgcca tccggaaagg
     5821 gaccaaaatg gaagatctct gtttccaatt cagtgaacac cccgcgatat tccaatttac
     5881 cgggcgagcc gctgcaggcc gcatccacac atacagatgg aaggatcggt ttaacactcg
     5941 cctcgcgcca ttttccgtgc gaagcaggtt tccctttgaa ctcttcatat tgagatcggt
     6001 aagccgcttc tgcttctttg agagaatcaa aggctttgta ctgcgcgccg gcaaatccct
     6061 tcacctgctt ttccgcctcc gcccatgcgg tgaaaatacc gggcttgcgt cccttccaga
     6121 cgacgtaata cttttgcttt ggcatgttca ttccgcagac cctaaaggtc tttgcccatt
     6181 aattttgatc tggagaatag tttacataga cctttagggt ctatgtaaac tattcttctt
     6241 tctcctgcca gcccgtttct tcgactgcca taaaaatatc ggtagcggag atcaaaccga
     6301 tcaacgagcc actttcatca gcaatgggca agtgatggat gtgtttctcc tgcatgacct
     6361 gcgaacactc catcaacgtc caattcgggt tgccagtaac gatcggaccc gacatgatct
     6421 cgctgaccgt ggtctcagat gggttgcgat ttgctgcaac gatcttgtcg cgtatgtctg
     6481 tggacgtcac aatgccatag gttgggtttt tttcgctgct ggccaccacc acactgtgaa
     6541 ttttgcggcg gcgcatcaag gtcaacgcgt acttgacgct cgaatcggga tcaacaacta
     6601 caaccgggct ggacatccaa tcacgaactg tgtttggcat atactcctcc aaattatttc
     6661 gtagggcggg atggcatccc gccctacata ttattgtaca accaattcaa ccgaatccat
     6721 cgcaaataac gaaccatcag cggcactgat atcctgcact tcaatgcgga tcaccgcgcc
     6781 cgataacgcg gtcccgatcg gaatgacggt gtcaaacgtt cccggtaaac ccaagtctgg
     6841 tgccgcaaca gcaattgcac ccgcagagag ctcaacaccg ccgacatcat aaatgcgata
     6901 caccagatta ttttcgaacg gtgcaatcgc gacctcaccc ttaatttgga cagagctgta
     6961 ggtcgctgtg ccagaagcag gtgacgtgat cgtgatggtg cgaggtcttc cgtcgggagt
     7021 gaaggtggtg tagtcaatca tatccagttg accgtcgata aaacgaaagt gacgcgtggt
     7081 cttcaacgtt gggcagcaca tggggtcttc aaaaccatga gtgatggcat ccaggtagat
     7141 ttcctgatct tcaatcaaga gcgcgtttat ttgcggacga tcatccacaa gtttcgacgt
     7201 ttgaaaaaca agatctccac ctacttccga atagaccgcc aggaaaacaa acgtgccagt
     7261 acccccgtaa ttttcactca ccaatgcaac gacttcgttc tggccatcgt cattcaggtg
     7321 tccacgtgca atgaaatccg ttacaaagac agagatgaaa tcagtgcttc ccgaaacacc
     7381 tttttcgtat tttccatcgc ttaactggac gatctgtaag gagtcgggga ttcccagttg
     7441 atattgcgtg ttacgaattt catccacgaa agcgggcgcg ggtgtcggcg acccaatg
//
LOCUS       DASTJP010000672         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_988007, whole genome shotgun sequence.
ACCESSION   DASTJP010000672 DASTJP010000000
VERSION     DASTJP010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988007"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFQ23_19610"
     CDS             complement(<1..764)
                     /locus_tag="VFQ23_19610"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET9908867.1"
                     /translation="MDISVRRLAQVIINWSEEHPRSMPWKQTADPYKIWISEIILQQT
                     QVAQGTAYYRAFLRRFPTVKHLARASEDEVLSAWQGLGYNSRARHLHTAARTIAQDLK
                     GRFPKNYDQIRALKGVGEYTAAAIASFAFGLPTPVLDTNVIRVLCRVRGITDDPSKRG
                     TRESLYAILNTMIRDIDPGQFNQAMMNFGAMQCKARNPDCENCVLAKSCVAFREGLVE
                     VLPVRAKRVQRRLRFFHYFVIHDNHSVVIRKRTTKD"
     gene            complement(821..1345)
                     /locus_tag="VFQ23_19615"
     CDS             complement(821..1345)
                     /locus_tag="VFQ23_19615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9908868.1"
                     /translation="MSALEFHSQFDQFTKVLQGFAYNLTKNREEAKDLYQETAFRALN
                     NQDKFIPGTNLKAWLLTIMKNIFINNYRKKVKANTIIDNTENQFLLNSGSNAIDNDAE
                     SQILMDEITEMIEHLDDSLKVPFEMHRIGYKYQEIADKLRLPLGTVKSRIFFARKELK
                     EMIEARYPKVLDRF"
     gene            complement(1554..2645)
                     /locus_tag="VFQ23_19620"
     CDS             complement(1554..2645)
                     /locus_tag="VFQ23_19620"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET9908869.1"
                     /translation="MLSLFDRYIIRQYFVTFFFSAFLFTLIALAIDFSERVNNFIEHK
                     LSARQVIVEYYLNFIPWINGVLWPLFALIAVVFFTARMAKNSEMIAALGAGMSFTRLL
                     RPYLISAFVLATIHFLGSHIFIPNGNKTFIRFDNTYIHPGNARTKTDNIHIFLTPDSR
                     IFIRNYRSRDTSMREVFLEKFDSVRLVTLIKAEKMQLLTPPNTWRLSNYEIRNFDNEF
                     KETITVEQAGFLDTTLNLEPRDFVRYINQSEMMTTKEIREFIAYERSKGLGTAQQMQS
                     EVQRRSADPFTILVLTVIGAAVAARKVRGGVGVHLASGIIIGASYILLSRFAMTFASQ
                     LEIHPALAIWVPNFIFTTVAIWLVLRAQK"
BASE COUNT          731 a          638 c          622 g          758 t
ORIGIN      
        1 atatcctttg tggtccgctt ccgaattacc acgctatggt tgtcatggat cacgaagtaa
       61 tgaaagaacc ttaagcgacg ctgcacacgc tttgcacgaa caggcagaac ctcgaccagg
      121 ccttcgcgaa aagctacaca ggattttgca aggacacagt tttcacaatc aggatttcgt
      181 gccttgcact gcatcgcgcc aaaattcatc attgcctgat tgaattgacc tgggtcgata
      241 tccctgatca tggtgttcag aatcgcatag aggctttcgc gcgttccgcg tttagaagga
      301 tcatccgtta ttcctctcac cctgcagagc acccggatca catttgtgtc taacaccggc
      361 gtcggtaatc caaacgcaaa tgatgcaatt gctgcagccg tgtattcccc tacaccttta
      421 agagcgcgga tctgatcata attcttagga aaccgtcctt tcaggtcctg tgcaatcgtg
      481 cgtgccgcag tatgtaaatg ccttgctctt gagttgtacc ccagcccctg ccatgcgctc
      541 agcacttcat cctctgatgc tctcgccaga tgcttcactg taggaaagcg tcttaggaat
      601 gcacgatagt atgcagtgcc ttgcgcgact tgtgtctgct gaagaataat ctccgagatc
      661 cagattttgt aaggatcagc agtctgcttc catggcattg atctgggatg ttcctccgac
      721 caattgatga tgacttgagc aaggcgtctg acagagatat ccatatcggc gaatatccgt
      781 catgatccgg aacccgggag aaaaagaaac tgcctggaaa ctagaaccga tccaacactt
      841 ttggatatct ggcctcaatc atttccttga gttcctttct ggcaaagaag atgcgactct
      901 ttacggtgcc aagaggcagt cgcagtttgt ctgcaatttc ctggtatttg tatccgatac
      961 gatgcatttc aaaaggcacc ttcaggctat cgtcaagatg ttcgatcatt tctgtaatct
     1021 catccatcag gatttgcgat tcagcatcat tgtcaatggc attggaccca ctgttcaaca
     1081 gaaactggtt ttctgtgtta tcaatgatgg tattggcctt gactttcttg cgatagttgt
     1141 tgatgaagat gtttttcata atcgtcaaca gccatgcttt caggtttgtg cctggaatga
     1201 atttatcttg attgttcagc gcacggaatg ccgtctcctg atagagatct ttcgcttctt
     1261 cccgattttt cgtcaggttg taggcaaagc cctgcagaac cttggtgaat tgatcaaact
     1321 gtgaatgaaa ctctagtgct gacatgatat acctccgaat tgattttgtg ttcgttttct
     1381 aaatcgaatc aaagataatc ttaggttgtg caaaaagcaa tactctggtt gttcaaaagt
     1441 ggttaaatca agttaacatg gtcatatcca tacttcgata tgtaacaaga cattgattta
     1501 caggatatta taaaatatgc ggaagcgtta aagttttcaa aatattttga aagctatttc
     1561 tgcgcccgta agaccagcca aatcgcaacc gttgtgaaga tgaaattggg cacccaaatc
     1621 gccagtgccg gatggatctc aagctggctg gcaaaggtca tggcaaaccg ggaaaggaga
     1681 atataagagg caccgatgat aatcccggag gccagatgca ctccaactcc gccacggact
     1741 tttcgtgcgg ccaccgcagc accgatcacc gtgagaacaa ggatggtaaa gggatcggca
     1801 gatcgacgct gtacctcgct ttgcatctgc tgggctgtac caagcccttt ggatcgttca
     1861 taggcaataa actccctgat ctcttttgtt gtcatcattt ccgactgatt gatatatcgc
     1921 acgaaatccc ggggctccag atttaaggta gtatcgagaa aaccagcctg ctcgacggta
     1981 atggtttctt tgaactcatt atcgaaattc cggatctcat agtttgacag ccgccaggta
     2041 tttggtggag tcagtaactg cattttttca gctttgatga gtgtcaccaa ccggacgctg
     2101 tcaaattttt caagaaacac ctcacgcata ctggtatccc ggctcctgta gttgcggata
     2161 aagattctgg agtccggtgt caagaagatg tggatgttat ctgtttttgt cctcgcatta
     2221 cccggatgga tgtatgtgtt gtcaaaccgg ataaatgttt tgttgccatt tggaataaat
     2281 atgtgactac cgaggaagtg gatcgttgcc aacacaaatg ctgaaatgag atacggccgg
     2341 agcagtcgtg taaagctcat tcctgctcca agcgctgcta tcatttcaga attctttgcc
     2401 atccgcgctg taaaaaacac tacagcgatc aatgcgaaca acggccaaag aaccccgttg
     2461 atccatggaa taaagttcag gtagtactca acaatgacct gcctggcaga aagcttatgc
     2521 tcgataaaat tgttcacccg ttcgctgaaa tcaatcgcca gcgcaatcaa cgtaaacagg
     2581 aacgctgaaa agaagaacgt cacaaaatat tgccggataa tatagcggtc aaagagggag
     2641 agcatacttt gtcatcatga gttttaagct ggtcgatgcg tcaaaactaa cgctatttgt
     2701 ctccgtaccc gtgccctgga ggatggacat atttacaagc gctgatcca
//
LOCUS       DASTJP010000673         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_705028, whole genome shotgun sequence.
ACCESSION   DASTJP010000673 DASTJP010000000
VERSION     DASTJP010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705028"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFQ23_19625"
     CDS             <1..588
                     /locus_tag="VFQ23_19625"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9908870.1"
                     /translation="AQRIHDAIPNVKIVASLRNPADRAFSGFLMRTRRGEPVKGFYEE
                     LTADASHVRESLYHKRLKRYFDLFPRDQIKIIIFEEFRKNPAQTLVDLFSFLGVDPNF
                     VPDTSVQHNPAGVPKIRLLNKLFFHPTLIRTAKAIFPESVHERLKQVQQQNLKAPPKF
                     PPDLRAKLLDIYREDINKLEGLLGRDLSIWLNKVS"
     gene            715..2301
                     /locus_tag="VFQ23_19630"
     CDS             715..2301
                     /locus_tag="VFQ23_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908871.1"
                     /translation="MRKVLLLALIVLVIIIALAVLMNSNQSLQPMMTVEPLTSPAVTV
                     LPSPTLNAPTFISPTSISAAAAQVAAFPGAEGFGAQSLGGRGGTVIEVTNLNDSGPGS
                     LRACVDTQGPRICVFRVGGTITTESEIVAANPYLTIAGQTAPGGGITLRAAQNYHKEP
                     FIISTHDVVIRFVRFRAGASAEPNSSRRSLTINFGAYNVMLDHCSFSWATDQPLLLID
                     GVHNVTIQWSIISEGLARSTHFEDGAFQEHSTGLSVSGKNYNSTAQTGNITIHHNLLA
                     HNGGRNPQNAGYGLEDVVNNVIYNWGRQAFTTQDLQADVPTNIVNNYFKAGIDSVGYE
                     VQAHHNNNSMMGPRVYVSGNIGPNRTDDTQPNISIVKPADRDFLVSSPFPAAPVTTTA
                     AEQAYQDVLSNAGARIPSLDAVDKRIIEEVKAGGGRIIDCVSANELTSPIDCATRVYL
                     SPYDYTRYGINDPLDEMGWPILAAGTPLQDSDRDGMPDSWETSLGLNPNADDSAQDRD
                     GDGYSNIEEYLNGLAGDLVS"
     gene            2551..3411
                     /locus_tag="VFQ23_19635"
     CDS             2551..3411
                     /locus_tag="VFQ23_19635"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9908872.1"
                     /translation="MTLPTFLGIGVQRGGSTWLHTLLASHPDVYMPTIRKEIRFFDQY
                     YDNGLAWYESFFCSSEEAVKYQAIGEISPEYYLCEQCPERIFKTFSQVKMIIMLRHPV
                     SRAYSQYGFTVQRRNYRGSFEEFLAARPGALEKGFYSRYIKRYFQFFDRRQFLPLLFE
                     DVFTDLPKTKNTIGDFLGISSEKFPEFDRNEKVNASTVPTHRSLYDFVVKTGRRLRKR
                     NLEPVVDFVMRLGIQRFLSKGEPLQPLDKELKKKLSEAYYDEFDELEKCLQIDLSSWR
                     IEKPMKERTL"
     gene            3412..>4042
                     /locus_tag="VFQ23_19640"
     CDS             3412..>4042
                     /locus_tag="VFQ23_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908873.1"
                     /translation="MIVEFVGSTGAGKTSLISKIHHKLAQTATVTTQFDLIAAPLNLK
                     SVSHPTVQNLIQEVVAFPFFVASLNRHKKFLTHTIRTFSNNTKLSLYKINNLRSLERK
                     IGLYEISQRYNRDQIILVDEGPILAAHMFVFTGSRVQDSDLATFAELLPLPDLVVYIK
                     APIEVLVRRALKRPDPPREMGSKDPVLTEKYVRSATAVFDRLMEAGRIKS"
BASE COUNT         1108 a          903 c          999 g         1032 t
ORIGIN      
        1 gcacagcgaa ttcacgatgc catcccgaac gtgaaaatcg tggccagcct gcggaatccc
       61 gccgaccgtg ccttttccgg attcttgatg cgcacgcgtc gcggcgagcc cgtcaaggga
      121 ttttatgagg agttaacagc agatgccagt cacgtccgcg aaagtttgta ccacaagcga
      181 ttgaagcgat attttgatct tttcccgcgc gatcagatta aaattatcat cttcgaagag
      241 ttcagaaaga atccagccca aactcttgtg gatttgttta gtttcctggg cgttgaccca
      301 aacttcgtgc cagatacatc tgtacagcac aatcctgccg gcgtcccaaa gatcagattg
      361 ctaaataaat tgttttttca cccaacgttg attcgcacag ccaaggcgat atttccagag
      421 agtgtgcacg aaagactgaa acaagttcaa caacagaact tgaaggcacc tccaaaattc
      481 ccgccagact tgcgagctaa attgttggac atttatcgcg aagatatcaa caaactcgaa
      541 gggttgctgg gacgtgatct ttcgatctgg ctcaataaag tttcctgatc agcagcggaa
      601 atattcctag gaatccaaga gaatccaact cccggcggta tgagcaatta gctcatccac
      661 gggaaaccct gttgcatgaa tagagaccaa agaagttctt cgaatgctgg accgatgcgg
      721 aaagtgctgc tgctggcatt gatcgtattg gttatcataa ttgcgctagc tgttttgatg
      781 aatagtaatc aatcgcttca accaatgatg accgtagagc ccctcacttc acctgcagta
      841 actgtgttgc cgtcgcctac tcttaacgcg cctacgttca tctctcccac ttcgatttct
      901 gctgcggcgg ctcaagttgc ggcttttcct ggcgcagaag ggtttggtgc gcagagcctg
      961 ggcggtcgtg gcggaacagt aatcgaagta actaacctca acgacagtgg accgggcagc
     1021 ttacgggcct gtgtggatac acaggggccg cgcatttgtg tattccgcgt aggcgggacg
     1081 atcacaactg aaagtgagat cgtcgccgcg aacccctatt tgacgattgc aggacaaacc
     1141 gctccaggag gagggataac tctacgggct gcccaaaatt accataaaga accgtttatt
     1201 atcagcacac acgacgtggt catccgattt gtgcgcttcc gagcaggtgc atctgctgaa
     1261 cccaattcca gccggcggag tctcacaatc aactttggtg cctataatgt gatgctcgac
     1321 cattgttcgt ttagttgggc gacggatcag ccgttgttgt tgattgatgg tgtacataat
     1381 gttacgatcc agtggtcgat catttcggag ggattggcac gcagcaccca cttcgaagat
     1441 ggtgcattcc aggaacatag tacaggatta tccgtatcag gcaaaaacta taactcaaca
     1501 gcgcaaactg gcaacatcac aatccatcac aacttgcttg cccataacgg tggtcgcaat
     1561 ccccagaatg ccgggtatgg gctggaagat gtggttaata atgtgatcta caactggggt
     1621 agacaggcat ttaccaccca ggatttgcaa gcagatgtgc ccactaatat cgtgaacaat
     1681 tacttcaagg caggtataga cagcgttggt tatgaagtcc aggcgcacca caacaataat
     1741 tcaatgatgg gtccacgtgt ttacgtgagc ggcaatattg ggccaaatcg cactgatgat
     1801 acacaaccta atatctctat agttaagcca gcagaccgcg attttctggt cagtagtccg
     1861 tttccggccg cacctgtgac cacaactgcc gcagaacagg cttaccagga cgtactgtcg
     1921 aatgccggtg cgcgtatccc aagcctggat gcggtggata aacggatcat tgaagaagtc
     1981 aaagcaggag ggggccggat tattgattgc gtcagtgcga atgagctgac gagcccaatc
     2041 gactgtgcta cacgagtgta tttgagtcca tatgattaca ctagatatgg catcaatgat
     2101 ccactcgacg agatgggctg gcccattctg gctgctggca cgccacttca ggatagtgac
     2161 cgtgatggga tgcccgatag ctgggagaca tctctgggac tgaatccgaa tgctgatgac
     2221 agtgcacagg atagagatgg cgatgggtac agtaatatag aagaatatct aaatggattg
     2281 gcaggcgatt tagtatcatg atggtagagt tagttggcaa caatgcactg gcgagacagc
     2341 gctaattcca gaaggccggt gcgtgttagc aaaaccttgg aggcgatgga tgatgtgaga
     2401 tcttaaatat atttttaagc ttgaagaaaa tttaggtcaa gacctatccg gttttctgga
     2461 tgggacctag tgctcataaa aaaaccatat tgctcggaag aatgtcttgt aatttgtgac
     2521 aggaacgatt cttcagagga ggatgtcaat atgacgttac ccacattttt gggcattgga
     2581 gtacagcgag gcggctctac ttggttgcat acattgttgg ccagccaccc tgatgtatac
     2641 atgccaacga ttcgtaaaga aattcggttt tttgatcaat actatgataa tggccttgcg
     2701 tggtatgaaa gctttttttg ttcttcggag gaggctgtaa aataccaggc aattggcgag
     2761 atctcccctg aatattattt gtgcgagcaa tgcccagaaa gaatttttaa aacattctct
     2821 caggtcaaga tgatcataat gttgagacac ccggtgagta gggcgtattc ccagtatggg
     2881 tttactgttc agcgaaggaa ctaccgtggt tcctttgaag aatttttagc agcccgccca
     2941 ggcgccctgg aaaaaggatt ttatagtcgt tatatcaagc gttattttca attctttgat
     3001 agaaggcagt ttctccctct gttatttgag gatgttttta cggatttgcc aaaaaccaaa
     3061 aacacaattg gagattttct tggtatctct tccgaaaaat ttccggaatt tgacagaaac
     3121 gaaaaagtga acgccagcac agtgcccacg caccgatctt tgtacgactt tgtcgtaaag
     3181 accgggagaa gacttcgtaa gaggaatttg gagccagtgg ttgattttgt catgcgactg
     3241 ggcatacagc gattcttatc aaaaggggaa ccactccagc cactggacaa ggagttgaaa
     3301 aaaaagctta gtgaggcata ttatgatgaa tttgatgaac tggagaaatg tttacaaatt
     3361 gatctgagta gctggagaat tgagaaaccc atgaaggaaa gaaccctctg aatgatcgtg
     3421 gagtttgttg gcagcaccgg tgccgggaaa acgtcgctga tatcgaaaat tcaccacaaa
     3481 ctggctcaaa cagctacggt gacaactcaa ttcgatttga ttgcggcgcc gcttaacttg
     3541 aaatcggtaa gccacccaac ggtgcaaaat ttgatccagg aagtcgtcgc ctttccgttt
     3601 tttgttgctt cactaaatcg acataaaaaa tttctaaccc atacgatcag gaccttttcg
     3661 aataatacga aattatcgct ttacaagatc aataatctga ggagtttgga acggaaaatt
     3721 ggactctatg aaatatctca acgctataat cgcgatcaaa tcattttagt agacgagggt
     3781 cctattcttg cggcacatat gtttgtcttt accggctcac gtgttcaaga ttcggatctc
     3841 gcaacatttg ctgagttgct cccgttgcct gacttggttg tatacatcaa ggctcccatt
     3901 gaagttcttg tcaggcgtgc cttgaagagg cccgacccac ccagggagat gggatccaag
     3961 gaccccgtcc tgacggagaa atacgtccga agcgcgacgg cagtttttga tcgattgatg
     4021 gaagccggga gaatcaaaag cc
//
LOCUS       DASTJP010000674         5684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_584306, whole genome shotgun sequence.
ACCESSION   DASTJP010000674 DASTJP010000000
VERSION     DASTJP010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584306"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2680
                     /locus_tag="VFQ23_19645"
     CDS             <1..2680
                     /locus_tag="VFQ23_19645"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908874.1"
                     /translation="AYYELIELGGQSCILSMFYEITEQKKSQKALQRQLEELSVLHSV
                     ATAGTESFSEDEIIERVTKITSQLYPEVCGILQLNGKGDTLVPHFSYIGADISNWQDG
                     FPITSGVSGKAMRLGRTIRIGDVTNESEYIQIASDIRSELCVPIRVHERIIGILNVES
                     RKAQAFEESDERILNTIAGGLGTALEKLRLFKEEQRRRQEAETLRRAATAISSTLDLN
                     FVMEDILKALKDVIDYDSASVFLHEKEGLRLAVCQGVNEPERWINQIFPEDNELFEAI
                     RVNRTSIIIENVQQDRRFKNWGNTPDVHGWMGTPLISRGRVIGYITLDNHKTAAYDES
                     NVETAMAFAYQAAAAIENARLYDETQIRLSELETINRLSASLRTTQSVNSMLEILLNE
                     TLNLIKTEHGAIWLYDPAVDKLIQRAERGVPVSFKTKAVKTSENSIVGKAFLSGKIIT
                     SSEYISDPLSIPQNREVTPAGMSGICIPIQSTAGTMGVLAIGMENGYELTNQLGLLTT
                     LTEIAGNSIHRAQLFEHNREQIKRLTTLRDIDSAIASSFDLRLTLNILMDQTMKHLGV
                     HAINIGLYHQDLQSITFLGALGFQSSNVTRPQTRVRDGLAGQVIIRQKTFHITDLQNS
                     PEIKNEPLLKREGFVTYIGIPLIVKGQIKGVFEVFHRTSLLPTPDWMEFLHTLAGQAA
                     IAIDNSQLFENLQRSNQELIQAYDTTLEGWARALELRDRETEGHTRRVTDLTLRLAKY
                     MGISDYEMINIHRGVLLHDIGKMGVPDHILKKTGALNEEEWHEMRQHPLYAYNLLSPI
                     SYLRGALDIPYCHHEHWDGSGYPRGLKGEQIPLAARIFSVVDIWDALLSDRPYRKAWE
                     PSQVVDYLREIAGTHLDPNVVEVFLTMIAEGESKMG"
     gene            complement(2704..3915)
                     /gene="tyrS"
                     /locus_tag="VFQ23_19650"
     CDS             complement(2704..3915)
                     /gene="tyrS"
                     /locus_tag="VFQ23_19650"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET9908875.1"
                     /translation="MNIEEQVELLMQGTEYGDEELKKSMTAELRQRLLDAQKEGRPLR
                     VYCGYDPTSTDLHLGHTITMRKLRQFQDLGHEITFLIGSYTALVGDPSDKNRARPILT
                     EEQVSENSKTYAEQAYRVLDKQKTKIRYNGEWLSELSLVDLIRLGQNFTVQQFLAREN
                     FANRLEKGEPIFLHETFYALMQGYDAVAMQTDVQVGGSDQLFNIIVAGRKLQEALGQK
                     PLVGIITGILPGTDGIQRMSKSTGNIVPINTSAEDMYGKLMSIPDLAMSKYMRLATRW
                     SPQEIETFEKELASANLHPRDAKMKLAREITSVFYGDADAQKAEDAFIKMFQQKEVPE
                     EMPEHSLKTGQTVLDVILDAKLTSSRSEARRLLEQKGVRLDGETIERGDIVFPHPGVL
                     QVGKRKFLRVK"
     gene            complement(4057..4719)
                     /locus_tag="VFQ23_19655"
     CDS             complement(4057..4719)
                     /locus_tag="VFQ23_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908876.1"
                     /translation="MKRILILIALLLAACTPLAAPPDVSTGPTAFIDPSYPTVESAPV
                     TSSQLTSGIEVRADRAWRDGKQINVDVCYTLLDGSDWSIWGATLQYADAAVIDFGSTL
                     LSLQEPATGQAGQRCDTLSFLNVPPDADLSAVTFTVDAIAAPPRAEDYCTIYMPKIQQ
                     TLTERGVAISLNCADVNGVQAMQIVGKPDTMSQEEAEQIVYSDEFYTVKGPWSFTFNL
                     GQ"
     gene            complement(4852..>5684)
                     /locus_tag="VFQ23_19660"
     CDS             complement(4852..>5684)
                     /locus_tag="VFQ23_19660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HET9908877.1"
                     /translation="EVLILTRRARKPTAEFEWDGKTPDGWGHLVNEVDAVVHVAGLGL
                     ERWPWTQKQKQNFIDSRVLPGRALVSAIESASRRPRVFLQSSGINRYGLRGDRIADES
                     TPPGPDFLAQMTVPWEAATEPLESLGVRRVVTRNAVVLAKGDGLFPLIALPMKLFFGG
                     KFGSGEQAMPWVHISDQVNAVRYLLENDNAQGPYNLISPTPTSNAVFMRTIAKAFRRP
                     YWFHMPRLLLQLTLGEMSVLLTEGRYAQPKRLIELGFQFQFGELENAMEDLLVRTTTG
                     "
BASE COUNT         1500 a         1396 c         1365 g         1423 t
ORIGIN      
        1 agcctattat gagttaatcg aactaggcgg acaatcctgc attctgtcta tgttttatga
       61 aatcactgaa caaaagaagt cacagaaggc gctgcagcgg caacttgagg aactatcggt
      121 gctgcattct gttgctactg ctggaacgga aagtttttct gaagatgaaa ttattgaacg
      181 agttacaaaa attactagtc agctttatcc agaggtttgc ggcatccttc aactgaatgg
      241 taagggcgat accctcgttc ctcatttttc gtatataggg gccgatattt caaattggca
      301 ggatggattt ccgattacca gtggggtctc cggtaaagcc atgcggcttg gtcgaacaat
      361 tcgtattggc gatgtcacaa acgagtcgga gtatattcaa atcgcatcag acattcgttc
      421 agagttatgt gtcccgattc gtgtccacga gcgcatcatc ggcatcctga atgtcgagag
      481 cagaaaagca caagccttcg aagaaagcga tgaaaggatc ctcaacacaa tcgctggcgg
      541 acttgggact gccctggaaa agctgcgact ttttaaagaa gaacaacgtc ggagacagga
      601 ggctgaaacg ttacgacgag ccgccactgc catatcctcc acactggacc tgaactttgt
      661 gatggaggat attttaaagg cacttaaaga tgtaatcgac tacgatagcg ctagtgtttt
      721 tctccatgaa aaggagggtc tacgactggc cgtatgccag ggtgtaaatg agcctgagcg
      781 gtggatcaat caaatatttc cggaagacaa tgaattgttt gaagcgataa gagtaaatag
      841 aacgtccata ataatagaga atgtacagca ggatcgacga ttcaagaatt ggggaaacac
      901 gcctgacgtc catggctgga tgggtacgcc actcataagc cgaggcagag tcatcggcta
      961 tataactctc gacaatcaca agaccgctgc ctacgacgag tctaacgtcg aaaccgcgat
     1021 ggcttttgcg tatcaggcag ctgcagcaat tgaaaacgca cgcttgtacg atgagacaca
     1081 gattcgctta agcgaacttg aaactatcaa ccggctttct gcttccctgc ggacgactca
     1141 atccgtgaac tcaatgctgg agattttgct gaacgaaaca cttaatctaa ttaaaaccga
     1201 gcacggcgcg atttggctat acgaccccgc cgtagacaaa ctcattcaac gcgcggagcg
     1261 cggcgtccct gtaagcttta agacgaaggc agtcaaaaca agcgaaaata gtattgttgg
     1321 caaggcattt ctttcgggaa aaataataac ttccagtgaa tacattagtg accccctatc
     1381 cattccacaa aaccgcgaag ttacaccagc agggatgagc ggcatatgca tcccgatcca
     1441 atcgacagca ggaaccatgg gagtgttagc gattggcatg gaaaatggat atgaacttac
     1501 gaatcagttg ggactcttaa cgaccctcac tgaaattgca ggaaattcta ttcatcgggc
     1561 tcaattattt gaacataatc gagagcaaat aaaaagactt acgactcttc gcgacatcga
     1621 ctctgcgatt gcatcctctt ttgacctgcg tttgactctg aacattctga tggatcaaac
     1681 gatgaaacat ctgggtgtcc atgcgattaa catcggtctg taccaccagg atctgcagag
     1741 tattactttt ctgggcgctt tagggtttca gtcatccaac gtcactcgtc cacagactcg
     1801 agtcagagac gggctcgctg gtcaggtcat catcaggcaa aagacatttc atatcactga
     1861 cttgcaaaat tcgcctgaaa ttaagaacga acctttgcta aaacgtgaag gatttgttac
     1921 ttatattggc attccattaa tcgtcaaagg gcagatcaaa ggcgtctttg aagtattcca
     1981 tcgcacctcc ctcttaccaa ctccggactg gatggaattc ctgcatacac ttgccggtca
     2041 agcagcgatc gcgattgaca attctcaatt attcgaaaat ctgcaacgct ccaatcagga
     2101 gttgatccag gcctatgaca ccacgctgga gggctgggca cgcgcgttgg aactccgcga
     2161 ccgagaaaca gaaggtcata cgcggcgcgt gactgactta acgctgcgcc ttgcaaaata
     2221 tatgggtatc agtgactatg agatgatcaa cattcatcga ggcgtcttgc ttcacgacat
     2281 cggaaaaatg ggcgtcccag atcacatcct aaaaaagacg ggagccctaa atgaagagga
     2341 atggcatgaa atgcgccagc acccgttata tgcctacaat cttctttccc ctatatcgta
     2401 cctgcgcggc gctttggata ttccctattg ccaccatgaa cattgggatg ggagtggata
     2461 cccgcgcggc ttaaagggtg agcaaatccc cctggcggcg cgcatctttt cagtcgttga
     2521 tatatgggac gcgctgctct cggaccgtcc atatcgcaag gcatgggaac catcacaggt
     2581 ggttgactat cttcgggaga tcgccggaac gcacctcgat ccgaacgttg tagaggtctt
     2641 cttaaccatg atcgcagaag gcgaatcaaa aatgggatag cttaaaagct atcccatttt
     2701 tttttacttc actcgaagaa acttacgctt ccccacttgc aaaacgccgg gatgaggaaa
     2761 aacgatatct ccgcgttcaa tggtttcgcc atccaatctg acaccttttt gctctaagag
     2821 gcgccgtgcc tcgcttcggc ttgaggtcaa ttttgcatcg aggatcacgt ccagtaccgt
     2881 ctgcccggtt ttcaaagaat gttcaggcat ttcttctggg acttcctttt gctgaaacat
     2941 tttaataaag gcatcttctg ctttttgggc atcggcgtca ccatagaaaa cgctggtgat
     3001 ctcacgcgcc agcttcattt tggcatcgcg gggatgaaga tttgcggacg ccaattcttt
     3061 ttcgaaggtt tcaatttcct ggggactcca acgcgtagca aggcgcatgt acttgctcat
     3121 tgccaggtcc ggaatcgaca ttagctttcc gtacatatct tcggcgctgg tattgatggg
     3181 aacaatgttt cccgtcgact ttgacatacg ctgaatacca tccgtgccgg gtagaatgcc
     3241 tgtgataatt cccaccaaag gtttttggcc aagcgcttcc tgtaattttc gaccggcgac
     3301 gatgatgtta aacagctgat ccgacccccc tacctggaca tcggtctgca ttgcaacagc
     3361 gtcatatcct tgcattaaag cgtaaaaggt ttcgtgcaga aaaataggtt cacctttttc
     3421 cagacggtta gcgaagtttt cgcgcgctaa aaattgctgt acagtgaaat tctgacccaa
     3481 ccgaatcaaa tctaccagtg aaagttcgct taaccattca ccgttataac gaatcttagt
     3541 cttttgctta tccaaaacgc ggtaggcctg ctcggcatac gttttcgaat tttcagaaac
     3601 ctgttcctcg gttaggatag gacgcgctct gttcttatca gacggatcac ccaccagggc
     3661 ggtgtagctc ccaataagaa aagtaatctc atgaccgagg tcttgaaact ggcgcaactt
     3721 acgcattgtg atcgtatgtc cgaggtgcag atcagttgag gtagggtcat atccgcaata
     3781 gacgcgcaaa ggtctgccct ctttttgtgc gtctaacagg cgctggcgca gttcggcggt
     3841 catggatttc ttgagttcct cgtcgccgta ttctgtgccc tgcatgagca attcaacttg
     3901 ttcttctatg ttcatatgaa cctcccgcag cttcccagaa aataaaaacg cgcccgcgat
     3961 ggggggcgcg ttctacacac catcgcagcg tttaccgatg gatgtaataa taattgtttg
     4021 cagacttcaa accgagcatg tccgtattat acaacatcac tgtccaagat tgaaggtgaa
     4081 cgaccacgga cctttgacgg tatagaattc atcactgtaa acaatttgtt ccgcttcttc
     4141 ctgagacatt gtgtcgggtt tgccaacgat ctgcatcgcc tgcaccccat tcacatccgc
     4201 acagttgagc gaaatagcaa cgccgcgttc agtcagcgtc tgttgaattt ttggcatata
     4261 aattgtgcaa taatcttcag cgcgtggcgg tgcggcaatc gcgtcgacag tgaacgtcac
     4321 cgcagagaga tctgcatcag ggggcacgtt gaggaaagac agggtatcgc agcgttgacc
     4381 tgcctggccc gtggccggtt cctgcaagct gagaagtgtt gagccaaagt caatcacagc
     4441 cgcatctgca tattgcaagg ttgctcccca aatcgaccaa tctgacccgt cgagcagggt
     4501 ataacaaaca tcgacattga tctgcttgcc atcgcgccag gcgcggtctg cgcggacttc
     4561 gattccgctc gtcagttggc tggaggttac cggggccgat tcaacggttg gataggacgg
     4621 atcaataaaa gcagtgggtc ccgtagaaac atctggtgga gccgccaatg gagtacaggc
     4681 tgccagaagc aaagcgatca agattaatat acgtttcatg gaatctccta attgttgtac
     4741 cgctggtttc agcaattatc taaacttgaa ctttgaatca tagactacga attatcttca
     4801 agagttagta attatgcggc acttcgtaat tgagtcaggc ttcctgcctg actagccagt
     4861 agttgttcga accagtaaat cttccatcgc attttccagt tcgccgaact gaaactggaa
     4921 gcccaattct atcaaacgtt tgggttgtgc gtagcgacct tcggttaaga gaacgctcat
     4981 ttcacccaac gttaattgta gcagcaaacg aggcatatga aaccaatatg gacgccgaaa
     5041 ggctttcgca atcgtgcgca tgaaaactgc atttgaagtt ggcgtcggtg aaattaaatt
     5101 ataaggtccc tgcgcgttgt cgttctcaag caaatatcga acagcgttga cctggtctga
     5161 gatgtgtacc cagggcattg cttgttctcc actaccaaat tttccgccga aaaataactt
     5221 catcggcaaa gcgatgagag ggaacagccc gtcgcctttt gcaagcacta cggcattgcg
     5281 ggtcaccact ctgcgcacac ccaggctttc aagcggctcc gtagcagcct cccatggaac
     5341 ggtcatttgt gccagaaaat caggtccggg tggtgtcgat tcgtcagcaa ttcgatctcc
     5401 gcgcagacca taacgattga tcccggagga ttgcaggaaa accctggggc ggcgcgaagc
     5461 actctcaata gcggagacaa gcgcacgtcc cggaagcacg cgtgaatcga tgaagttctg
     5521 tttttgcttt tgagtccagg gccaacgctc caacccgagc ccggctacat gaacaactgc
     5581 atctacttca ttgaccagat gcccccaacc gtctggtgtt ttgccatccc attcgaattc
     5641 agcggtgggt ttgcgagcgc ggcgagtgag gatcaatact tcgt
//
LOCUS       DASTJP010000675        16587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_255335, whole genome shotgun sequence.
ACCESSION   DASTJP010000675 DASTJP010000000
VERSION     DASTJP010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16587
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255335"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFQ23_19665"
     CDS             <1..645
                     /locus_tag="VFQ23_19665"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9908878.1"
                     /translation="RIEDAPLGDVLKRRIFDPLGMKDTGFSVPKEKKDRRAGMYGFDE
                     AGRLIKRLTATGNSTLPERPDDMKYEGGGGGLWSTLDDYLAFARLFIGAGTVNGVRLL
                     RPETLTLMASNHLTQSQRGDANLLSSGHGFGLGVAVVLDSDKADPILCGGNVGSVGWP
                     GAWGGWWQADPSDNSVLIFLAHNMVELDQLAQGVGFGVFDAILRFQSLASRLPQ"
     gene            complement(741..1337)
                     /locus_tag="VFQ23_19670"
     CDS             complement(741..1337)
                     /locus_tag="VFQ23_19670"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9908879.1"
                     /translation="MRKIIVLEHISMDGVIQAPGGPDEDTSGGFAYGGWAAPYSDDVV
                     GTALRKQMSLPFDLLLGRKTFDIWAQYWPYHTDIWPGVKTATKYVASNTMTSHEWRPS
                     VFLGGDVAQKVAEIKQGQGPDLHVWGSGDLLQTLIKHDLVDNFWLMIYPITLGEGKRL
                     FADGTIPAAFKVTDSTVGSSGVIVMNYERAGAVTTGSA"
     gene            complement(1951..2781)
                     /locus_tag="VFQ23_19675"
     CDS             complement(1951..2781)
                     /locus_tag="VFQ23_19675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9908880.1"
                     /translation="MKTKTIVLVHGLSASKHSWDQWVSRYQSRGYKVIAPAYHPGLDK
                     SMAELKQNPNDPLLSTITLPQVLDYLTKVIKGLDEKPIIMGHSFGGLLTQLLLQRDLG
                     AAGVAVDGAPPPGIPPTQLSFLRFALPIFNPLIPSSKPWYMTFEQFQFAWAHTLPLAE
                     QRAAYEAVIVPESRGLYRSGLSSDAKVDWTRPRAPLLIIAGEKDHIFHPAVNEANQKR
                     YAKSPSVTDFKVFPGRTHYTVVAGKGWEDVADYALDWSLRQNVLAEGVKATRPVAATA
                     "
     gene            complement(3083..4042)
                     /locus_tag="VFQ23_19680"
     CDS             complement(3083..4042)
                     /locus_tag="VFQ23_19680"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9908881.1"
                     /translation="MSETIVLEPEAQAFAEATAQPPFLFELGPEKGRMAVDQVQSGPM
                     SKLPVTIEDLTIENGPSGPLSIRILRPENAATNLPVLLYIHGAGWVFGNHHTHDRLIR
                     ELAVGARAAVVFPNYSLSPEARYPIAIEENYSVLKWIVKHGQVQGLDAERLAVAGDSV
                     GGNMSAAVTLMAKERGGPRIHFQLLFYPVTNAAFDTDSYHQFAEGYCLRRDEMMWFWN
                     QYTTNPGERNEITASPLRASTEQLSDLPPALIITAEADILRDEGEAYANKLRDAGVRV
                     TAARFQGIIHDFVLLNALANTAAARGAIALATTWLRERFYGGA"
     gene            complement(4128..4958)
                     /locus_tag="VFQ23_19685"
     CDS             complement(4128..4958)
                     /locus_tag="VFQ23_19685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9908882.1"
                     /translation="MKQNLLMELRLWSTIRRAVVIALAIAFLVGSVGAVNAMTSNAKN
                     SDKPNIVLVHGAWADGSSWSAVIKALQLKGYNVIAPQFPLTSTADDVARLRQVLAVQD
                     GPTIVAGHSYGGQIMTSLGTDAPNVVGLVYIAAFGLDEGESIGGLLSHGPMPPALANL
                     RIDAQGYAWIPQSDFVNHFASDVNPVSANVMYAVQQPANLGILGQVMGTPAWRSLPSW
                     YMVAQNDEAIPPDAQRFFAQRMGATTVEIKSSHVPLVSRPESVANLIEQAAATVSSAP
                     "
     gene            complement(5109..5648)
                     /locus_tag="VFQ23_19690"
     CDS             complement(5109..5648)
                     /locus_tag="VFQ23_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908883.1"
                     /translation="MQIISCIPLVEEILETRKLNFENDYAGYKNHVYRMINFCFALRD
                     CDKEESEKLIIAGCFHDIGIWLGNTFDYLHPSIALAKEYLKQNGLEHWHVEMELMIDM
                     HHKLMPYRDERYPLVEVFRKGDLVDFSLGIVKCGLPKSFVNRVKSQFPNAGFHKRLVQ
                     LELSWVAKHPLNPLPILKW"
     gene            complement(5691..5930)
                     /locus_tag="VFQ23_19695"
     CDS             complement(5691..5930)
                     /locus_tag="VFQ23_19695"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9908884.1"
                     /translation="MLPTDKLTIQESVILSLLARGQRNALIADKLCISIRTVETHLYH
                     IFDKLGVSSRTEAAIYALRHGLISLSEISGNSEDI"
     gene            6152..12187
                     /locus_tag="VFQ23_19700"
     CDS             6152..12187
                     /locus_tag="VFQ23_19700"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HET9908885.1"
                     /translation="MKNSYRDRDYEFGQAILALRTKIDFTQARLANYLGVSRRAVVDW
                     EAGNSYPKAEHLKSLIGLAVKERTFPAGQEAEEIRALWNRAHQKVWLDELWLQELLTP
                     QASNAHHGIESSPKQMMDDFYRRDLLIEPTPLIGRDSELVEIARILTNPACRLLTILG
                     PGGVGKTRLVLEFAAGQMDSYNDGVAFVPLASVSLLDQVVSAIGDALDLSFVGQADPT
                     KYLLHYLRNRHMLLVLDNFEHLIESALLVQSILQHAPHITILVTSRTRLQLKAEWLFE
                     VEGLSYPHSVEPGSSQALTNLSNYGAVQLFAQRVAQVQPSLSITESTLNTIVHICQHV
                     AGIPLALELVAAGVRTHSIDEIEQEIQVNLDTLSTTLQDVPSRHRSMRAVFEHSWNLL
                     SAEERAAFSRLAIFRGGCTAEAAMQVTEASNSTLLALVDKSLLSQSSNRLGLTSDASP
                     RFTFLEPIREYALEKLMERGEKDLLQRSHMSYYMTLIESISQGWDTPLSETAIEQLQR
                     EYDNLRAALQAAHSLGDVEVGLRLAVSLGRFWRSRGHISEGRGWLEELLSPVANKLGP
                     STKNIQLQAIEQAAWLATDQHDFKRAEQLFKQAQSLRRELDLPEDDTYLIVNLALQAR
                     SVGEYRRATALLEDAVVRHRALRQRGGKGSADIGLSLYTLALVLREQGNFARASVLFE
                     EGIDFFRKSGELEGVAQGILGLSDVARDNGDVPRLREYCEKSHPIFVEFGTQWAIGYT
                     LHNLALADYMEGNFSQAIVLIDASMALFRAIQNDAALAEILIARGRILHAQGELVTAS
                     EVLTEALQLAWEMGPGLMLAAALEGLAGVTLDLGKTNRAVRMIRAASDFRLRMGTPLR
                     PVDQPEINEMLKNAQTFLGADSFTAIWESVSDEPLDHILSMLPVPMTSTAVSEIRTNI
                     QDLAIPVPKRNNYWEDAPSVPAFYGREWEINLLNEWIIDAHCRVVSVIGLGGIGKSAL
                     AVSFMHRVAEHFEIVIWRSIRDLPDCDTLLESLLQVVAPQALADVNTSLEHRQSFLME
                     YMRNYRILIVLDNLESALDEGEHAGNFLSGYEGVGRFLRLSAETQHNSCILLTSREKP
                     IDLAAREGNQSLVRTLRLARLDKKACEKLLAEKNVRGDPVQISQLIDAYAGNPLALSI
                     VAQTIVDLFGGEIAPFLEQGEVMYGGVRNLLEEQFIRLSALEQSILIWLAILREPATL
                     DELLTALVTPLPRARLIEALESLYRRSLIERGQKYGSFTLQSVVLEFATSRLITESTN
                     EIREGRLVRLIDHGLELAQAREDVRQTQERLILLPILNALHSAFHQQSLIEEHLLELL
                     SHLRTQADNTQGYGPANLVTLLRLSKGNLRGLDLSELSLRSTYLQGVEMQGTTIAKSV
                     LQGSIFTETFDAITAVAINNTEAYLAAASRDGRILLWEAKTLALRRVWQAHADMVWTL
                     GFNYDGGMLASGSWDGTVKVWNVSNGELLWLQGHSSHVNSVAFSPDGQTLATSGSDAT
                     VRLWEVKSGRQLQVLPHPVPVPVVAWSPDGHLLASGDMEGSIYLWEIRKDGPADNVKT
                     FVGHTNWVDGLAFAPRSNLLASASWDGTVKLWDIATRQLQQTLDGHKDRVVRVAWSPD
                     GHLLATASRDHTVWLWDVEEGSYKTILRGHTAGVNGIAFTSDNHRLLSGGEDGTLRVW
                     NVTSGNCIQVVRGHASALYDVDWSPDSTQLISGGTDYLVTIHSLTAEIAPRVMRGHTG
                     VVLGVGWGSNGRLASSEWDNIIHLWNAETGSLLQVLRHPADHGNYFYGLAWSPDGQRL
                     ASATYRHGIQIFEPDTHHQHWVGSPFPTWIRRVAWSPDGRQIAGGGDDGTVHIWDSTT
                     GTLLHRLVGSRGRVTDVAWSPDGSQLASGSSDKETGELFIWDTRRGERVYTGKHPGII
                     YAVAWGPNKSQVVTGGSDGNLRWWDIKRGKSVLIQEAHQGTIQSIKRSPDGKRLVSCG
                     NDGAIMIWDLGNGDHIQTLRRDRPYERLNISKIRGLTEAQKETLRALGAFEKD"
     gene            complement(12225..12761)
                     /locus_tag="VFQ23_19705"
     CDS             complement(12225..12761)
                     /locus_tag="VFQ23_19705"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9908886.1"
                     /translation="MINDQIFKTQLAESEWLLGRSNAHVTILIYGDFECPYSAMALPV
                     LESLVDEYPDSICLIYRHFPLNMSHRKARLAAEAAEAAGAQSMFWEMHDMIFSHQDQI
                     TFENLRRFARLLRLDGERFDHDIMGHRYRDEVQSDLLSGIQDGANGTPSLFVNGQRYR
                     GLLDRASLLSAVAAQLAA"
     gene            complement(12758..13333)
                     /locus_tag="VFQ23_19710"
     CDS             complement(12758..13333)
                     /locus_tag="VFQ23_19710"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HET9908887.1"
                     /translation="MINTLAHNWWTLVLRGLAAILFGILAYVLPDITFTVLVLLFGAY
                     ALWDGVFALIGAVRAQGERRWILVLEGLVGIAAGLVTFFWPGAATLAILTIMAVWAIV
                     TGVFEIIGAIRLRKEIEGEWFLLLSGLLSVLFGLALVIWPVAGLVAVTWMIGAYAIIF
                     GILLIVLGFRLRNWSDTSFTPPTAKTAGVSK"
     gene            complement(13430..13867)
                     /locus_tag="VFQ23_19715"
     CDS             complement(13430..13867)
                     /locus_tag="VFQ23_19715"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9908888.1"
                     /translation="MNYTDVDYPRVLAAHTIFGEKVVNLAGEHVGNIKELMIDLDDGL
                     IAYAVLSFGGFLGMGDKLFAIPWEALTINEENHSIILDVDKEVLKNAPGFDKDNWPDN
                     AKYEAGWLLGVYEYYGFAPYWMPDEEDVADQLEDETRQEFHPD"
     gene            complement(14143..14475)
                     /locus_tag="VFQ23_19720"
     CDS             complement(14143..14475)
                     /locus_tag="VFQ23_19720"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9908889.1"
                     /translation="MKNKISENGNNHGGIGTVMTSLVVGSVIGATVGLLMAPTSGEKT
                     RKKIQSEVKGAQKKAKAAVGNIETKSRELVDDVKGNVENVRETITERVTGVKKNTRAK
                     KWKVFGRR"
     gene            14708..14833
                     /locus_tag="VFQ23_19725"
     CDS             14708..14833
                     /locus_tag="VFQ23_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908890.1"
                     /translation="MLLDQSFLFIVLTVAFLGLSGFVLIKTQIIKSRLGYSWSKR"
     gene            14953..15930
                     /locus_tag="VFQ23_19730"
     CDS             14953..15930
                     /locus_tag="VFQ23_19730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9908891.1"
                     /translation="MVKLGYFLSSEEHRPGQLIRHAQLAEQSGFEFAVISDHFHPWVS
                     EQGQSAFVWSVLGGIAQVTEQIRIGTAVTCPIMRIHPVLVAQAAATVADMMPGRFFLG
                     LGTGEYLNEHILGEVWPRYDTRKEMLVEAIHIMRELWKGEDYSYDGDHFTVREARIYT
                     LPDELPPIYVAASGDESAEMAGELADGLISTSSGDEIVKAFTKNGGSNKPRYGSVKVC
                     WAKTQAEAKKTLEKEWPVSALSGRLHVDLATPKHFEDTVEAMGKVEIPEDSVIGPRPE
                     PYIEAIQSLQQNGFTHIYLHQVGPDQDGFLDFFQSTLLPQLEEANLVRE"
     gene            16072..16566
                     /locus_tag="VFQ23_19735"
     CDS             16072..16566
                     /locus_tag="VFQ23_19735"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9908892.1"
                     /translation="MASKVENLEDLYEDLLKDLYSAEKQLVKALPKMVKNADSPDLQK
                     AFKSHLGQTEKHVDRIERIFSSLEGSPRGKKCVGMEGLIAEGDELMKEDVDPDVLDAG
                     LIAAAQKVEHYEIASYGTARAWAEKLGYPDHVRLLQQTLDEESTANDKLTDLAESFIN
                     AEAQ"
BASE COUNT         4180 a         4040 c         4265 g         4102 t
ORIGIN      
        1 cgcatcgaag atgccccgct gggcgatgta ctcaaacggc ggatttttga cccgctcgga
       61 atgaaagata ctggcttcag tgtaccgaag gagaaaaaag accgacgagc gggaatgtac
      121 ggttttgacg aggcggggcg cttaatcaaa cggctcaccg ccacgggcaa ttcaactctg
      181 ccggagcgac ctgatgatat gaaatacgaa ggtggcggcg gcgggctttg gtccacgctg
      241 gatgactatc ttgcctttgc acggctattt attggcgcag gcacagtgaa tggcgtgcgg
      301 ctgttgcgtc cggaaactct cacattgatg gcatcaaatc acctgacgca aagtcagcgt
      361 ggcgatgcaa acttattgag ttcaggacat ggattcggac tgggggttgc ggtcgtgctt
      421 gactctgata aggctgaccc aattttgtgt ggcggtaacg ttggttcggt cggttggccg
      481 ggcgcatggg gtggctggtg gcaggctgat cctagcgata actcggtgct cattttcctg
      541 gcacacaaca tggtggagct cgaccaattg gctcaagggg ttggctttgg cgtgttcgat
      601 gccatcttac gttttcagtc tctggcgtca cgccttccgc agtagagatg cgcctcgaac
      661 ctgacgatcc ctgcactaac agttggaaga tatcaaccta ttcaattctt tgaacctgtc
      721 gcccgaaaag gcgcgaacct tcatgcactc ccggttgtga ctgcgcccgc acgctcataa
      781 ttcatgacaa tgacgccact cgaaccgact gtgctgtccg tcaccttgaa cgccgcggga
      841 atcgtgccat ccgcaaacaa tcgctttcct tcccccaacg ttatcggata gatcatcagc
      901 caaaagttat cgaccaaatc atgcttgata agtgtctgaa gcagatctcc acttccccaa
      961 acgtgtaaat ccggaccctg cccttgtttg atttcagcga ccttttgtgc aacgtctccg
     1021 cctaaaaata cagaaggccg ccattcatga gacgtcatcg tattcgaggc gacgtatttg
     1081 gtagccgttt tgacacctgg ccatatgtcc gtatgatacg gccagtactg tgcccaaatg
     1141 tcaaaggttt tgcgccccag caacaggtcg aacggcaagc tcatttgctt tctcagggct
     1201 gttccaacaa catcatcgga atatggcgct gcccatccgc catacgcaaa accaccgctg
     1261 gtatcttcgt ctggtccgcc tggggcttga ataaccccat ccatggagat atgttcaagc
     1321 acaataattt ttctcatcac tcagttcctc caatatctgg attaagatct ggttgcatcg
     1381 ctccagacag gtaaacgacg tctcgacgtt tacgttactt catgaggcag agcacaaatg
     1441 caaaatagcc aggtcaatag taacgacccg tccggtcttt gctatctttc gtgtcgagcg
     1501 cggctgtatt ctaacccagg gcttcggcat cttgttaaga aaaccctgtc ggtgtgccat
     1561 attgagaggg aaatgacgat aaatcagaca aatagaatcc tggtattccg ctcaacagga
     1621 ggaaccattc ctcttggatt gatttgtggc aacgaatcgg aatcgctctg atgatttcga
     1681 agaccctagt cacgatcccc cagacggcta tgagggcaag ggttgctgca ttttgctcag
     1741 gttcgcttgt ttaaagtaat agtgaatctt gttcgataac tttgtggatg cgcgaaacgt
     1801 ggcatcagtc ggagaggcgt ttttgttata ccctaggcca gttcggcatc aagcggactg
     1861 ataaaacata tttattccaa cgtctgcggc aagttatagg attgccgcag acgttggagt
     1921 taaaaactgt ctcgtactga cttatttttt ctacgctgtg gcagctacag gcctggttgc
     1981 ttttacgcct tcagccaata cgttctgtcg aagtgaccag tccaaagcgt agtctgcaac
     2041 gtcttcccag cccttacccg cgacgactgt gtagtgcgtt ctaccgggaa agactttgaa
     2101 gtcggtgacc gatggtgact tggcataccg tttttggttt gcttcgttga ccgcggggtg
     2161 aaagatgtga tccttttctc ccgcaatgat cagcaagggc gcccgaggac gagtccagtc
     2221 cactttagca tcggaggaca agccgcttcg gtagagcccg cgtgactcgg gcacgatcac
     2281 cgcctcatag gcggcgcgct gctcagccag cggtaaggta tgagcccacg caaattggaa
     2341 ctgctcaaag gtcatgtacc agggctttga agaagggatc aacgggttga agatcggcaa
     2401 tgcgaagcgg aggaatgaca actgagtcgg tgggatcccc ggaggtggag cgccatcaac
     2461 ggcgacgccg gcggcgccca gatcgcgttg aaggagaagt tgagtcaaca gtccaccaaa
     2521 cgagtgaccc ataatgatcg gtttttcgtc aagtcccttg atcaccttgg tcaagtaatc
     2581 cagcacttga gggagcgtaa tcgtgctcaa tagtggatca tttgggtttt gtttgagctc
     2641 agccattgat ttgtcgagtc ctggatgata cgcaggggct atcactttat atccacgaga
     2701 ctgatagcgg gaaacccatt gatcccagga atgcttgctg gcagacaggc cgtgaaccaa
     2761 tacgattgtt tttgttttca tgatcattat cctttctact tttaagatct ttttagaacc
     2821 tggtttgaga tggcgatatc gtagccgata gtaatccatt tgtcatcaga acttctacgt
     2881 atttttcaca gggagttttg atggcgtgag agggctggta tctaagccat cctgttcact
     2941 gaaatgataa tcttgtaaac cgggcatgtg cggtgacatg gtcaacaggc gggtttgttg
     3001 gagggcagga tgatctacgt ttcatagaaa atactttccg ccgatgttat aagccaggtg
     3061 aatcgtccga ttcctctttc gatcacgcac ctccataaaa gcgctcacgt aaccaggtag
     3121 ttgccagagc aatcgctcca cgtgctgccg ccgtatttgc cagcgcgttc agcaggacga
     3181 agtcatggat gatgccctga aatcgagccg ctgtgacgcg tactccggca tcccgcagct
     3241 tattagcata agcctctcct tcgtcgcgca ggatgtccgc ttcggcagtg atgattaagg
     3301 caggtgggag gtcactaagt tgctctgtgc tggctcgcag aggcgaggcc gtgatctcgt
     3361 tacgctcacc cgggttggtc gtatattgat tccagaacca catcatctcg tcgcgtcgaa
     3421 ggcagtaccc ttcagcaaac tgatgatacg aatctgtatc gaaggcagcg ttggtcaccg
     3481 ggtagaacag gagctgaaaa tgaatcctgg gaccgccacg ctccttagcc atcagcgtta
     3541 cggcggcgga catattgccg cccacgctgt ccccagccac cgcgaggcgc tctgcatcca
     3601 acccttggac ttgtccatgt ttaacgatcc acttcaatac tgaatagttc tcctcaatag
     3661 ctatcggata cctggcttca ggcgatagac tgtaatttgg aaaaacgacc gcagcccgcg
     3721 caccaaccgc gagttcccgg attagccggt catgtgtatg atgattgccg aatacccatc
     3781 cagcaccgtg aatatacaga agaacaggga ggtttgtcgc ggcattctct gggcgtaaaa
     3841 tccgaatgga tagtggtccg cttggaccgt tttcgattgt gagatcctcg atagtcacgg
     3901 gaagcttgct catcggccca gactgtacct gatccacagc catgcgccct ttttcaggcc
     3961 ccagttcgaa taaaaaggga ggctgtgccg ttgcctccgc gaaggcttgg gcttcaggtt
     4021 ccaaaacaat tgtttcagac attgtttgtt tcctctcaac gagtgaaact tgccgaaggc
     4081 aggaatggta gcgtatcttc ctgccttcgg aatgaattcc agattagcta tggcgctgaa
     4141 gaaacagtgg ccgctgcctg ttcaatcaag tttgcaacac tctcgggccg ggagaccagc
     4201 gggacgtggc ttgatttaat ttccaccgtg gtagcaccca ttcgctgggc gaagaaacgc
     4261 tgggcatcgg gcggaatggc ctcatcattt tgagcaacca tataccagga tgggagagat
     4321 ctccatgcag gcgtgcccat gacttgccca aggatgccaa gatttgcagg ttgttgcaca
     4381 gcatacatta cattggctga gaccggattc acatctgagg caaagtgatt cacaaagtct
     4441 gattgaggga tccaggcgta gccctgggca tcgatacgca aattggcaag tgctggaggc
     4501 ataggtccat gggaaagcaa accaccaatg gactcacctt cgtcaaggcc gaaggctgcg
     4561 atatacacca ggccaaccac attcggcgcg tcagtaccaa gcgaggtcat gatctggccg
     4621 ccgtaagaat gaccagcaac gattgtggga ccatcctgaa ctgcaagtac ctggcgtagc
     4681 cgtgccacat cgtcagctgt cgaggtgaga ggaaactgtg gtgcaataac attatatccc
     4741 ttcagttgaa gggcctttat aacagcgctc caactggaac cgtctgccca ggcaccatgc
     4801 accagtacaa tgttcggttt gtctgaattt ttggcgttcg atgtcatcgc gtttactgct
     4861 ccaacagatc caacaagaaa agctatggca agcgctatca ctactgcgcg ccggattgtg
     4921 gaccaaagtc tcagttccat tagcaaattt tgtttcatga tgtagctcct tttatctaag
     4981 attaggtgta tcgttcaaac catgcaggca tcttcctgca cagggccacc aaagctgaat
     5041 gtatcagcca ggtttgtccc acttcatgtt tgacagtttt cttcatgaac ctcctcgctt
     5101 cttatgcgtt accacttcaa aattggcaac ggatttaatg gatgttttgc aacccagctc
     5161 aactcaagct gaaccaatcg cttatgaaat cctgcatttg gaaattggct tttgacacga
     5221 ttgacaaacg atttaggcaa gccgcattta acaatcccaa gggagaaatc taccaagtca
     5281 cctttacgga atacttcaac cagcggataa cgttcgtccc ggtatggcat gagtttgtga
     5341 tgcatatcaa tcatcagttc catttcgaca tgccagtgtt ccaggccatt ttgtttgaga
     5401 tactccttcg ccaacgcaat ggatggatga aggtagtcaa atgtgttgcc gagccaaatc
     5461 ccaatgtcgt gaaagcagcc ggcgataatg agtttttcgg attcctcttt atcgcagtcg
     5521 cgcaacgcaa aacagaaatt gatcattcga tagacgtggt ttttatatcc ggcataatcg
     5581 ttttcaaaat tcagttttcg cgtctctaat atttcttcta ccaggggaat acagctgatg
     5641 atttgcatgg aactctccat ttatgtatgt tcagtgtagt gatgattgat ctatatgtct
     5701 tcagagtttc cactgatttc cgaaagggag atcaaaccgt ggcgtagtgc atagatcgct
     5761 gcttccgtcc ttgatgacac acccaactta tcgaagatgt gataaagatg cgtttcgaca
     5821 gtccgaatgc tgatgcagag tttgtctgca atcagggcgt tgcgctggcc ccgtgccaag
     5881 agagagagta tgacgctttc ctgaatggtt aatttatctg taggcagcat gagatatctc
     5941 ctgattctgc aaggtctaga ttatggaagt gcacaatgga aacataaggg aaatgtaatg
     6001 aagagtataa ttatccgaat aagataagtc acttaccacc aggtgacacc ttcacttccc
     6061 accacttcgc atttggaagt cacgacaaga atactcttct tgtttttgat tccgccgaca
     6121 ttaatttttt catattcaca aaagggttac tgtgaaaaat tcataccgag atcgagatta
     6181 tgagtttgga caggcgatct tggctttacg caccaaaata gatttcacac aggcgagatt
     6241 ggcgaactac ctgggtgttt ctcgacgcgc ggtagttgac tgggaagccg gcaatagtta
     6301 tcccaaagct gaacatctaa aatcgcttat tggtctggcc gtcaaagaac gcacctttcc
     6361 agcgggtcag gaagctgagg aaatccgtgc gttgtggaac agggcgcacc agaaagtctg
     6421 gctggatgag ttgtggctcc aggaacttct gactccccag gcgagcaacg cgcatcacgg
     6481 tattgaatcc agccccaagc agatgatgga cgacttttac cgcagagatc tgctcatcga
     6541 gcccacacca ctgattggac gagactcgga attggtcgaa atcgcacgca tcctaacaaa
     6601 tccggcctgt cgcctgctca caatacttgg tccggggggt gttggcaaaa cgcgcctggt
     6661 attggaattt gcagccggtc agatggattc atacaacgat ggcgttgctt ttgttccact
     6721 tgcctcagtc agccttttag atcaagtggt ttcagcgatt ggtgacgcgc tcgacctttc
     6781 atttgttggg caggctgatc cgacaaaata cttgctccac tatttgcgaa atcgacatat
     6841 gctgcttgtg ctcgataact tcgagcactt gattgagagc gctctacttg tacagagcat
     6901 acttcagcac gcgccgcata tcaccatcct ggtgacatcg cgcactcgct tgcaactgaa
     6961 agccgaatgg ctatttgagg tcgaaggtct ttcatatccg cacagtgtgg aacccggttc
     7021 ttcgcaagcc ctgacaaatc tctccaatta tggggccgta caactgttcg cccagcgcgt
     7081 ggcgcaggtg caaccaagcc tatccattac agagtcgacc ctcaacacga ttgtgcacat
     7141 ctgccagcat gtcgcaggaa ttccgctggc gctggaactg gtagccgcag gcgtccgaac
     7201 acactctatc gacgaaattg agcaggaaat tcaagtaaac ctggacacac tttccactac
     7261 tttgcaggat gtaccatcac gacatcgcag catgcgagcc gtgtttgagc actcctggaa
     7321 tctgttgagc gctgaggaac gtgccgcttt tagtagattg gcgatttttc gcggaggttg
     7381 tacagcggaa gcagccatgc aggtaacgga ggcatcaaat tcgactctcc tggctttagt
     7441 ggataagtct ctgctatcac agtccagcaa caggctgggt ttgacttctg atgccagccc
     7501 tcggtttacc tttctcgagc ctatccgcga atacgcgctg gaaaaactaa tggagcgagg
     7561 cgaaaaggat ttactccagc ggtcacacat gtcctactat atgacactga ttgaatctat
     7621 atcgcagggc tgggatactc cactgtcaga aacagctatc gaacagcttc agcgagaata
     7681 tgacaacctt cgcgctgcat tacaggcggc gcattccctg ggcgatgttg aagtgggtct
     7741 gcggcttgca gtctccctcg ggcgattctg gcgaagtcgt ggacatatca gcgaaggtcg
     7801 tggctggctg gaggaattat tatccccagt tgctaacaag cttggaccct caacaaaaaa
     7861 catacaactg caggcaattg aacaggcagc ttggttggca accgatcagc atgatttcaa
     7921 gcgtgctgaa caactcttca agcaagccca gtcattgagg cgtgaattgg atttgcccga
     7981 ggatgataca tatttgattg ttaatttagc actccaggct cgcagtgtag gagaatatcg
     8041 ccgggcaact gcgctattgg aagatgctgt cgtgcggcac cgtgctttgc gccagcgcgg
     8101 aggcaaaggt agtgcggata taggactttc tctttatacg cttgccttgg ttcttcgaga
     8161 gcaaggaaat tttgccaggg cttctgtcct gtttgaagag ggaattgact tttttcgtaa
     8221 gagcggcgaa cttgaaggtg tagctcaagg tatcttggga ctcagcgatg ttgcgcgcga
     8281 caatggcgat gtgccgcgtt tacgtgagta ctgtgagaag agccatccta tttttgtcga
     8341 gtttggaacg caatgggcaa tcggttacac actgcacaac ctggcactgg cagattacat
     8401 ggagggcaac ttctcgcagg cgattgtatt gatagacgcg agtatggctc tgtttcgtgc
     8461 tatccagaac gatgctgcac ttgcagaaat tctcattgcg cgggggcgga tattacatgc
     8521 tcaaggcgaa ttggtcaccg caagtgaagt ccttactgaa gcattacaac tagcctggga
     8581 gatgggacca gggttgatgc ttgcggctgc tttggaaggg ctggctggcg ttaccttgga
     8641 tctggggaaa accaatcgag cggtgcgcat gatacgtgca gcgtcagact tccgtcttcg
     8701 gatgggtacg cctctacgcc cggtggatca gccagagatc aatgaaatgc ttaagaatgc
     8761 tcaaactttt ctgggtgctg attcgttcac agcgatttgg gagtctgtct ctgacgagcc
     8821 gcttgaccac atcctcagca tgctcccggt acccatgaca tcaactgcag tgagcgagat
     8881 acgtacaaac atccaggatc tcgccattcc tgtacctaaa agaaataatt attgggagga
     8941 cgcgccctcg gtgccagctt tctatgggcg cgaatgggaa ataaaccttc tgaatgaatg
     9001 gatcatcgat gcacattgcc gtgtggtcag tgtgatcggt ttgggcggga ttggcaagtc
     9061 ggcactggcg gtcagcttta tgcaccgagt agcagaacat ttcgagatag tcatatggcg
     9121 ctcaatacgc gacctgcctg attgcgatac tctgctggaa agcctcttgc aagtagttgc
     9181 accacaggcg cttgccgatg tgaataccag cctcgaacac cgccagtcct tccttatgga
     9241 atacatgcgc aattaccgta ttcttatagt tttggataac ctcgagtcgg cacttgatga
     9301 aggtgagcat gcgggtaatt ttctttcggg atatgagggt gtcggacggt tcctccggct
     9361 cagcgctgag acacaacaca acagctgtat cttgctgacg agccgcgaga aaccgatcga
     9421 tctggctgcc cgcgagggaa accagtcttt ggtgcgtacc ctacgccttg ccagactcga
     9481 taaaaaagcc tgcgaaaaat tgctggccga aaagaatgtt cgtggcgacc cagtgcaaat
     9541 atctcagctc atcgatgcat atgcagggaa tccgctcgcg ttgagtattg ttgcacaaac
     9601 cattgtggat cttttcggag gggaaattgc gccgtttctc gaacagggtg aggttatgta
     9661 tggtggtgtc cgtaatctgc ttgaggagca gtttattcgg ctctctgccc tggagcaatc
     9721 cattctgatt tggctggcaa tcctgcgcga gccggccacg ctggatgagt tgttaacggc
     9781 cctggtgacc cccttacccc gtgcccgtct catagaagca ctcgagtctc tctaccggcg
     9841 ttccctgatc gaacgtggtc agaagtatgg cagttttaca ttgcaatctg tagtattgga
     9901 attcgcgacg tcgcgtctca tcaccgagtc caccaatgag atccgcgagg gtaggctggt
     9961 tcgcctcatc gatcatggac ttgagctcgc acaagcacga gaggatgttc gacaaacaca
    10021 ggaacgatta attttgttgc ccattcttaa cgctctgcat agtgctttcc accaacaatc
    10081 cctgatcgag gagcacttac ttgaactgtt gagccacctg cgcacccaag cggataatac
    10141 tcagggctat ggaccggcta acctggtaac tttattacgc ttgtcgaagg gtaatttgcg
    10201 cggactcgat ttatcggagt tgagtctgcg tagtacatat ctccagggtg tagagatgca
    10261 gggtacaaca attgcaaagt cggtgctaca gggcagcatt ttcactgaga ccttcgatgc
    10321 aataacggca gtcgcgatca ataacacgga agcttacctg gcagcggcca gccgagatgg
    10381 aagaatactc ctatgggagg ccaagaccct tgcactgcgg cgggtctggc aggctcatgc
    10441 tgatatggtt tggacacttg gtttcaacta cgatggaggc atgttggcaa gtggtagttg
    10501 ggatggcact gttaaggttt ggaacgtgag caacggtgaa ctactctggc ttcagggcca
    10561 ttccagccat gtaaattcgg tcgccttttc tcccgatgga caaacccttg cgacaagtgg
    10621 aagcgatgca accgtccgac tttgggaggt aaaaagcggc aggcagcttc aagtactccc
    10681 acatccggtt ccagtacccg tggtagcctg gagtccagat gggcacctgc ttgcaagtgg
    10741 ggatatggaa ggcagcattt atctctggga gatccggaaa gatggtcccg cagacaatgt
    10801 aaaaacgttc gtcggacaca cgaactgggt agatggattg gcattcgcac cgagatccaa
    10861 tttgctcgca agcgcgagtt gggatggtac tgttaagtta tgggatattg ccacgaggca
    10921 actgcaacaa acgcttgatg ggcacaaaga tcgggtcgtt cgcgtggcat ggagtccgga
    10981 tggacatctt cttgccactg ccagtcgtga ccacacggta tggctctggg acgtggaaga
    11041 gggtagctac aaaaccatac tacgaggcca tacggccgga gtcaatggaa tagccttcac
    11101 ctctgataat catcggcttt tgagcggagg tgaagatggt actttgcggg tgtggaatgt
    11161 cacaagtgga aactgcattc aggtagtgcg gggtcatgcc tcggcgctct atgatgtaga
    11221 ttggagtccg gatagtactc aattgataag tggcggcacc gattatctgg tgacgattca
    11281 tagtttgacg gctgagatag ctcccagagt gatgcgtggg cataccgggg tggtcctggg
    11341 tgtaggatgg ggatcgaatg gacgattggc aagcagtgaa tgggacaata tcattcatct
    11401 atggaatgcc gaaaccggtt ccttacttca agttctgcgc catcccgcgg atcatggaaa
    11461 ttatttctat ggtttggcat ggagcccaga tgggcagcgg ttggcaagtg caacctatcg
    11521 gcacgggatc cagatattcg agccggacac ccatcatcaa cactgggtcg ggagtccgtt
    11581 tccgacttgg attcggcgcg tcgcatggag tccggatgga cgccaaatag caggcggcgg
    11641 agatgatggc acagtgcata tctgggactc cacgactgga acactgcttc atcgattagt
    11701 gggctctcgc ggtagagtga ctgacgtagc ctggagtccg gatggatcgc aattggcttc
    11761 gggcagcagc gacaaggaaa ccggagagtt atttatctgg gatacgcgcc gcggggaacg
    11821 cgtgtacacc ggaaaacatc ctgggatcat ctatgctgta gcgtggggtc ccaacaagag
    11881 tcaggtagtc actgggggca gcgacggtaa tttgcgctgg tgggatatca aacgtggtaa
    11941 atccgtgctg atacaagaag cacatcaggg aaccattcaa tctattaagc gaagtcccga
    12001 cgggaagagg ttagtgagct gtggtaacga tggcgcaatt atgatttggg atctggggaa
    12061 cggtgaccat atccaaacac tgcgacgcga ccgaccttat gagcggctta atattagtaa
    12121 gattagaggg ttaaccgaag cccagaagga aaccctgcgt gctttagggg cctttgagaa
    12181 agattgacta tctccacgat taatccatga ggagtttggg gtaatcatgc cgccaattgg
    12241 gccgccacgg cagaaagaag cgaagcgcgg tcaagcagcc ctctgtaacg ttgaccatta
    12301 acaaataacg acggtgtgcc attggctcca tcctgaatgc cgctgagaag atcactttgt
    12361 acttcgtcgc ggtagcgatg ccccatgata tcgtgatcga agcgctcgcc gtccaatcgg
    12421 agtaaccgag caaaacgtcg caggttttca aaagtaatct ggtcctggtg ggaaaaaatc
    12481 atatcgtgca tctcccaaaa catgctctgt gcgccagctg cttcggctgc ctcggccgcc
    12541 aaccgggcct tgcgatgtga catattgaga gggaaatgac gataaatcag acaaatagaa
    12601 tccgggtatt catccaccag gctttcaagg acaggtaaag ccatagcaga atagggacat
    12661 tcaaaatcgc catagatgag aatagtcacg tgggcatttg aacgtcctaa gagccattca
    12721 ctttcggcaa gttgtgtctt aaaaatttga tcattaatca tttggagaca cctgctgtct
    12781 tggcggtcgg tggtgtgaaa ctcgtgtcac tccagttgcg cagacggaaa cctaacacaa
    12841 tgagaaggat gccaaaaata atcgcgtatg ccccgatcat ccaagtgacc gccaccaagc
    12901 cggcaaccgg ccagatcaca agggcaagtc caaacaagac ggagagcaga ccactcaaca
    12961 ggaggaacca ttctccttcg atttctttgc gcagacgaat cgccccgatg atttcaaaga
    13021 cgcccgtcac gattgcccaa actgccataa tggtaaggat ggcaagggtc gctgcaccgg
    13081 gccaaaagaa cgtgaccaag ccagcggcaa taccgaccag tccctccagg acaagaatcc
    13141 agcggcgttc gccctgtgcg cgaactgcac cgatcagcgc gaacacacca tcccatagtg
    13201 cataggcacc gaagagaagc acaagaacgg taaaggtgat atctggcagc acatatgcca
    13261 gaatgccaaa aaggatagcc gcaagcccgc gcaggacaag cgtccaccaa ttatgagcca
    13321 gggtatttat catgtttgtc tccacgtggt ttgaagtcgc agaagaaaaa gcaaattctt
    13381 atttcacagc ttgcctttta gacttgaata tgaagttcca accaagtaac taatccggat
    13441 gaaattcctg cctggtttca tcttctagct gatccgcaac atcctcctcg tcgggcatcc
    13501 aatacggtgc aaaaccgtaa tattcgtaaa cgccaagcag ccaaccggct tcatacttgg
    13561 cattatccgg ccagttgtct ttgtcgaagc caggagcatt cttcagcact tccttatcta
    13621 cgtcgagaat gatgctgtga ttctcttcat tgatagttag cgcttcccac ggaatggcga
    13681 atagtttatc gcccatcccc aggaaaccgc caaaggatag gacagcatag gcaatcaagc
    13741 catcatccag atcgatcatc aactccttga tatttccgac gtgttctccg gcgagattga
    13801 ccaccttttc accaaagatc gtatgcgcag ctaaaacacg aggatagtca acatctgtat
    13861 aattcatctg atactccttt tattattaag cgctgatggc tgtagctttc tcccaattgc
    13921 tcacattagt ttttctgaat tgttgtgcgc atgatttttc attacgctaa attgcaccgt
    13981 cagaacgcaa caaatttctc attcgtacac ccaattccaa tttacaccgt aagatctgcc
    14041 attcgcacag gccaatcgaa tatcttcaat tgggagcgcc ttaatgaaat ctataaggtt
    14101 tcaaccaaag agagataccc acaacgggta tctctctttt agttatcggc gaccaaacac
    14161 cttccatttt tttgctcggg tgtttttctt aactccggtg acgcgctccg tgatggtctc
    14221 acggacgttt tcgacattgc ctttcacatc atcgacaagt tcgcggcttt tcgtttcgat
    14281 atttccgaca gccgctttcg cctttttctg agcgcctttt acctcgctct gtatcttctt
    14341 gcgggttttc tctccggatg tgggagccat cagtagaccc actgtagcac cgattacact
    14401 accaaccacc aggctggtca tgaccgtgcc aatgccgcca tggttatttc cgttttcact
    14461 aattttattt ttcatgttct ctcctttgta aattgacgca taccttggag taaaaacttc
    14521 taaggcaatc acacttataa aagcaaactg aaaaaaattt tatggggaaa gttcctaata
    14581 aggttacggg gatgccctat aaaaaatggt ttaactccta ctttagagta ggaacatcat
    14641 gaattgaagt tcaaacccaa caagcgtata gtatgggcat acttaatcac gaagcaggag
    14701 acacaacatg ttacttgatc aatcttttct atttatagtg ctgaccgtcg catttttggg
    14761 tctttccggt ttcgtcctaa taaagactca gatcatcaaa tcccggctgg gttattcctg
    14821 gtcgaagcgt taactctgga atttcagaag aattccaaaa accgctttca gactagttaa
    14881 tgtttgagct ggcgaatgtt tcgtcagctc aaaccgctta ataatgaaat ttataaaaat
    14941 aaggagagta ttatggtcaa acttgggtat tttctatcca gcgaggagca tcggcctggt
    15001 caattaattc gtcatgcaca actcgctgaa caatcaggtt ttgagttcgc ggtgatctct
    15061 gatcattttc atccctgggt aagtgagcag ggacaaagtg cctttgtttg gagcgtcctg
    15121 ggcggtatcg cgcaggtcac cgagcagatc cgtatcggta cggcagtgac ctgcccaatt
    15181 atgcgtattc acccggtgtt ggtggcacag gcagccgcta ccgtggcaga tatgatgcca
    15241 ggtcgctttt ttctcgggct gggaacgggt gaatatctga atgagcacat tcttggagaa
    15301 gtatggccac gatatgacac tcgcaaggaa atgctggtgg aagccataca tatcatgagg
    15361 gaactttgga agggcgaaga ctactcctat gacggcgacc attttacggt tcgagaagcg
    15421 cgcatctaca ctcttccaga tgaactgcct ccgatttatg tagctgcctc cggagatgaa
    15481 tcagccgaaa tggctgggga gctggcggat ggattgatca gtacatcgtc tggggatgag
    15541 atcgtgaagg cgttcacgaa aaatggcgga tcaaataagc cccgttatgg ctctgtcaaa
    15601 gtctgttggg caaaaacgca ggcagaagcc aaaaagaccc tggaaaaaga atggcccgta
    15661 tctgccctct ctggcaggct tcatgtggac ctggctacac cgaagcactt tgaagatacc
    15721 gtggaagcca tgggcaaggt agagatccct gaagactccg tcatcggacc gaggccggaa
    15781 ccttatatcg aagccattca atctctgcag cagaacggct ttactcatat ttatctccat
    15841 caggtaggac cggaccagga tggcttcctt gacttcttcc agagcacgct gttgccgcaa
    15901 ttagaggaag caaacctcgt tagagagtga agctgcaaga aaaaatacac agattggtgc
    15961 aaatagggaa agacctacca agctttaacc gcatccccca ttgttcattc cagtgtcaaa
    16021 aactagaata caccagactg tgaaaacaaa aatttgagat tggagaaagt tatggcatca
    16081 aaagtggaaa atctggaaga tctctacgaa gacctgttga aagatttata tagcgctgaa
    16141 aaacaattgg taaaggcgtt accgaaaatg gtaaaaaatg ctgattcacc cgatctgcaa
    16201 aaagcattta agtcccatct tggacaaacc gaaaagcacg tcgaccggat cgaacgcatc
    16261 ttttccagtt tggaaggctc gccccgtgga aaaaaatgcg tcggcatgga agggttgatc
    16321 gcagaaggcg atgagttgat gaaggaagat gttgacccgg atgtgctgga cgccggcttg
    16381 atcgccgccg ctcagaaagt ggagcattac gaaatcgcca gttatggcac cgcacgagcc
    16441 tgggcagaga aattggggta ccctgatcac gtgcgcctct tgcagcagac ccttgacgaa
    16501 gagtcaacgg caaacgacaa gctgaccgat cttgcggaaa gctttatcaa cgcagaagct
    16561 caatagcctg aaaagactcg actactt
//
LOCUS       DASTJP010000676         9557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_62713, whole genome shotgun sequence.
ACCESSION   DASTJP010000676 DASTJP010000000
VERSION     DASTJP010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62713"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFQ23_19740"
     CDS             <1..342
                     /locus_tag="VFQ23_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908893.1"
                     /translation="ILGRSDQEVYVWAVCTSIISSGTSPISGTEIFSSPSIPAVIHLG
                     EDGSVQSVEIPGAGSDYARDISQMFPSDVQKEFNRYHFGRAGEMLEHAAWRRTHPDEP
                     PLIVLVSTPKP"
     gene            complement(370..786)
                     /locus_tag="VFQ23_19745"
     CDS             complement(370..786)
                     /locus_tag="VFQ23_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908894.1"
                     /translation="MSNKHQLVNWIITTLREAAWAPLGVVGFYAIGLAFDWYDRFPPL
                     DMPSHLLGGVAITYFYISAIKHSQRIVGEIPGLIQTLLAFTSTGTTIIFWEFYENAAD
                     WFLGTQMVRGLEDTIVDMLVGLTGALILVLLYKRRS"
     gene            1080..2234
                     /locus_tag="VFQ23_19750"
     CDS             1080..2234
                     /locus_tag="VFQ23_19750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9908895.1"
                     /translation="MKILTVLTYYRPHTSGLTIYAERLARAFSKRGHQVTVMTTQFDP
                     SLPREEIMDGIKVIRVPVAVRVSKGVLAPTFGLVATKLVAQHDVVQMHLPQFDAPGVA
                     LRGRLLGKPAVLTYHCDLLLPPGLFNRVVNMVVDVMNNTAGVLSNHIVTYTRDYADNS
                     PYLSRYASKLTPILPPVELPAPLPGAVEAFAEEHQVKERRPVIGMAARFAAEKGVEVL
                     LDALSAILQKYPKAQVLFAGTYQNVMGEHAYSDRLMPRIREYESAGHWTFLGNLDPVQ
                     MAAFYPNLDVLTVPSLNSTEAFGLVQIEAMMNGVPCVPSALPGVRQPVMMHKMGRVAK
                     IGDSADLAESILEVLSEPQKFKGDVEAIKKAYNPDSIAQEYEKLFKRLMG"
     gene            2234..3025
                     /locus_tag="VFQ23_19755"
     CDS             2234..3025
                     /locus_tag="VFQ23_19755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9908896.1"
                     /translation="MANPPPSIENRPSKDFLFLQLRDLPYFRAFLRAVESSYYQDLPL
                     PTPIYDVGCGDGHFASLTFDDKIDVGLDPWHGPIHEAKKYGAYKSLVEADGAKSPFPS
                     EYFASGFSNSVLEHIPHIDAVLAETARVLKKDAPFYFCVPNTRYLSELSISRLIGKGY
                     TEWFRRISRVQHADDPEVWGERLQRAGFEMIRYWHYFSPSAMRVLEWGHYFGVPSVFA
                     RLLTGRWIIARTEWNLWLTKSYVSRYASPEPVKDGTFTFYIAKKK"
     gene            3181..3894
                     /locus_tag="VFQ23_19760"
     CDS             3181..3894
                     /locus_tag="VFQ23_19760"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9908897.1"
                     /translation="MPFDEKYFSTHTYSDITFAKYSMYWWSNRFFALLARRYGKRGAR
                     LLEIGSGLGHLVGQLEDTFDTYGMDVNYWAVNQSKSVVHESALQAASAQELPYKDNSF
                     NVVIIKHIVEHLPDPKKAVAEIGRVTEKGGTLILATPNLDSLLKPWKGEKWIGYQDPT
                     HISLKKPAEWISFIKEAGFEIQRVFADGFWDVPYIPVVPKQIQKLTFGSLGGFQAITG
                     FVFLPMKWGESIIVIASKR"
     gene            3901..6039
                     /locus_tag="VFQ23_19765"
     CDS             3901..6039
                     /locus_tag="VFQ23_19765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9908898.1"
                     /translation="MSNELQNNNQLVEPSVLDYVKSLFRLGNGERIQIPDFVREDQEQ
                     PNLQEASHQPKVSFPWRSLLALLLALIGQTTFEPPANSAPLGVAFYVAALSMLGWAVY
                     RGEWTLAPQAQTSEHTDPLTYRLLPLFFSVVLGLWAFVILGNNFFTRTNVIIWGLAVA
                     FFVGSLWLKSGTGESVFYRVGNFLNRDSWTVNISRWTLLLLAATVVIFFFRFYQTTSV
                     PSEPFSDHAEKILDVYEVSQGQTHIFFIRNTGREAFQMYWTVLIANLFGTGFSFLSLK
                     LGTAILGFLTLPFIYLLGKEIGGPRVGLLAFFLVGIAYWPNVISRVGLRFPLYPLFVA
                     PTLFFLIRGLRTRNRNDFLLSGLFLGIGLHGYSPFRIVPIVVAAAFILYWLHSQSKGA
                     RRELFVWLAIVALTSLFVFLPLLRYWYDDPTMFGFRAITRVSGLEQPLPGPAHEIFFS
                     NLWNALKMFNVDDGEIWVHSVPGRPALDVVSAALFLLGVVLLLIRYIRTRHWLDLFLL
                     ISIPLLLMPSILSLAFPGENPALNRAGGAYIPAFMIAALALDGLLTTLGRGGIRTVMT
                     WILAGVLLYVAADRNYELVFDQYATQFRQASWNTSDMGKIVKEFEQTYGRTDTIWIVP
                     FEHWVDTRLPAVWAGIPDRDMAIWPENLPGTVDLTGPKLFMVKAELENPDLNDQASLD
                     ILQELYPNGNLRMFDSDIAGHDFWIFTVPQ"
     gene            6048..>9557
                     /locus_tag="VFQ23_19770"
     CDS             6048..>9557
                     /locus_tag="VFQ23_19770"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET9908899.1"
                     /translation="MNRDKYFWLFDILFLLVLALAGYLRLTGVAWGEGQHQHPDENHF
                     SSVLENLRARECAEPAIPIEACPEEQKRWISIGDYFNSKTSTLNQYNRGFSFYVYGNL
                     PMTITRVAAEALNTTNFRLFGRQISALADLFAIVFLYLSVSRLYGRRIGLLAALFSAL
                     TVMQIQQSHFFTVDLFVNAFAFLAIYFAVLILDYRQKEIRTTQVDAFTSSEMANDPEP
                     VVSKTPISSSSIDLANLKGLITHPLLLLSLGFGFAYGMALASKVNIYPLAILLPGALG
                     LRYLIANKEATDNGSGESTTNYWSWVIACLIAGGFAAFVSFRIFQPYAFDGLLPSEQW
                     IANIQEQRAQAKGDADLPWNLQWARRSHFYSFENLTTWGLGLPLGILAWAGFLYMGWR
                     ILKGEWRHALLWGWTGAYFAWQSLQFNPTMRYQLPIYPLLAMMAAWVIFEIPRNFQSE
                     KARSVVRSLSYVLGVIVLVLTAVWAFAFQSIYIRSEPRIAASRWIFQNIPGPINIQIQ
                     AEDSIYNQPLPFPSGVRIDSTMPYQTSFIAQSDGLVNEILFAHIAAQNQASPELYLTL
                     WQDLNDPQPLASAFTTISSENGFAGTGQVANFDQSPLLTANQIYYLKLEPSVLEGTVD
                     VCGPVNIQVQAVDQLVEQVVDVSEPCTVTADTPYIATFIPQVTGTLNTILLEHATNLS
                     LNEAVDPITLNLVVSKDPSVTAESSIARGSLTEQFSTSGDPRGGSYTLTLDQPVKVEK
                     DTQYYLQLEVDSGTLGLSGASISNETDYDYGLPFRLDGYDAFGGIYRGDLNLQVYWDD
                     NADKLNRFVTTLDQTDYIFIPTNHQYAQITRLPERYPLTTLYYRELMGCPEDKEIIWC
                     YHTAKPDQFKGRLGFDLVAVFETYPTLGPIVINDQWAEEAFTFYDHPKVLIFKKSENF
                     NKEQVGSILSTVDLANVVRLTPPQFSDYSNLLLSAEKLAQQRAGGTWSDLYSYEWLQN
                     RYPILGLLIWYLFIFVLGLAVYPLIRLTMPGLADKGYPLSRALGLVLFGYLAWVAGSI
                     GIPYTRLSITGTFGLILGVGILLARYQRDEIREEWRNNRKYFLMAEGIFLTFFLIDLL
                     IRFGNSDLWHPAKGGERPMDFSYFNAVLKSTSFPPYDPWFAGGYINYYYYGFVLVATP
                     VKLLGIVPSIAYNFILPTLFAIVG"
BASE COUNT         2259 a         2318 c         2332 g         2648 t
ORIGIN      
        1 attttaggac gatcggatca agaagtatat gtgtgggcgg tttgtacgag catcatttcc
       61 tctggaactt ctcccatcag tggaacagaa attttttcct ctccatcaat acccgctgtc
      121 atccatctcg gagaagatgg aagcgttcag agtgtggaaa ttccaggagc aggaagtgat
      181 tatgcccgtg atatcagcca gatgtttcca tcggatgtgc aaaaagaatt taaccgttac
      241 cattttggaa gggcaggaga aatgttagaa cacgctgcat ggagacgaac acatccagac
      301 gagccgccat tgattgtgct tgtctcaacc ccaaaaccat gactctcaga aagtaataaa
      361 agagctttgc tacgaacgcc gtttataaag taacaccaag atcagcgccc ccgttaaacc
      421 cactaacata tcaacgatcg tatcttcaag tccgcgtacc atttgcgtgc caagaaacca
      481 atccgccgcg ttttcataaa actcccaaaa gataatggtg gtccctgtag aggtaaaggc
      541 gagaagggtt tgaatcaaac ctggaatttc accgacgatg cgttgcgaat gtttgattgc
      601 tgagatgtaa aagtacgtga tcgctacacc acccaataga tggctcggca tgtctaaagg
      661 tgggaatcgg tcataccaat caaaagccag tccgatcgcg taaaagccga caacacccaa
      721 cggcgcccat gcggcttcgc ggagagttgt aatgatccag tttactagct gatgtttgtt
      781 actcattcat aacctcgatg tagattctac taacggtcgg atagtctgtt tctgaagtaa
      841 cccgctggtc gagtaacccc ggatactttc tgcggggtgt atcgagacca aaagtaccgg
      901 gccaacaaaa gatttgctgg aaagctgtgg tctcgatacg ccttcggcac tcgaccaacg
      961 gttcgctttg gaaacaaaac gttaggtacc aaaatgattc tatcaatatt aaagcctgct
     1021 catcgctctc ggttataatt tctccgcgct ccatcggggc ttgcgacttc caacactaca
     1081 tgaaaatact cacagttctt acttattatc gtccacatac ctccggactg acaatttacg
     1141 ctgagcgtct ggcgcgcgcg ttttccaaac gcggccatca ggtgacggtc atgaccacgc
     1201 aatttgatcc atcgttgccg cgtgaagaaa tcatggatgg tatcaaagtg atccgcgtac
     1261 cggtcgcagt gcgcgtcagc aaaggtgtgc tcgcacccac ctttgggttg gttgctacga
     1321 aattagttgc tcaacacgat gtggtgcaaa tgcatctacc tcaatttgac gcgccgggcg
     1381 ttgccttgcg cggtcgactc ctgggcaagc cggctgtgct gacctatcat tgtgacttgt
     1441 tattgcctcc cggactcttc aaccgcgtcg taaacatggt cgttgatgta atgaataata
     1501 cagcaggggt tttgtcgaat cacattgtca cctacacgcg agattacgca gacaattcac
     1561 cctatctctc acgctacgcc tcaaaactga ccccgatcct gcctccggtt gaactgccgg
     1621 ctccactccc cggcgcggtg gaggcatttg cagaagagca tcaagttaaa gagaggcggc
     1681 ctgtcatcgg catggcagct cgctttgccg cggaaaaggg tgttgaagtc ctcctggacg
     1741 cgctctcagc gattctccag aaatatccga aagctcaggt cttattcgca ggtacatacc
     1801 agaacgtgat gggtgaacat gcctactcag atcgactcat gccgcgcatc cgagaatacg
     1861 aatctgccgg tcattggacg tttctgggaa atcttgaccc cgtccagatg gcggcgtttt
     1921 atccaaacct ggatgtgcta acagttcctt ccctgaactc gaccgaggcg tttggtctgg
     1981 ttcagatcga agcgatgatg aacggggtgc cgtgcgtgcc gtctgcattg ccgggtgtga
     2041 gacaacccgt catgatgcac aagatggggc gcgttgccaa aattggggat tcagccgatt
     2101 tagcggaatc aattcttgag gtcctgagtg agccgcaaaa attcaagggg gatgttgaag
     2161 ccatcaagaa agcatacaat cctgactcaa ttgcgcagga gtacgaaaag ctattcaaga
     2221 ggttgatggg gtaatggcca atccgccgcc gtccattgaa aatcgtccat ccaaggattt
     2281 tcttttttta caattacgcg acctgcctta cttccgcgca tttttgcgcg cagtagaaag
     2341 ttcgtattat caggacttgc ctttacccac gccgatctat gacgtggggt gtggcgatgg
     2401 tcattttgcc tcactaacgt tcgacgataa gattgatgtt gggctcgacc cctggcatgg
     2461 gcccattcat gaggcaaaga aatacggggc ttataaatct ttagtagagg ccgacggtgc
     2521 aaaatccccg tttccgtctg aatattttgc cagtggcttt agcaactccg tgctggagca
     2581 tatcccgcac atcgatgccg tgctcgcaga aaccgcacgt gttttaaaga aggatgcgcc
     2641 gttttacttt tgtgtgccaa acacgagata tttaagtgaa ctctcgatct cgcggctgat
     2701 cggcaagggt tatactgaat ggttccgcag aatctcgcgc gtccagcatg cagatgaccc
     2761 cgaggtctgg ggagagaggc tgcaacgcgc cggatttgaa atgatacggt actggcatta
     2821 tttttctccg tctgccatgc gcgtgttaga atggggtcat tatttcggcg ttccatccgt
     2881 atttgcacgg cttcttactg gtcggtggat tatcgctcgc actgaatgga atctctggct
     2941 gacgaagagt tatgtgagcc gatatgcctc ccctgaacca gtaaaggatg gaacgtttac
     3001 attctatata gcgaagaaga agtaaaactg ccctgtagac aatcataaaa gctgaaattt
     3061 gtggttgtct attttccttg ttacggcgct ttgtatgaag tttttttata cagaggataa
     3121 ccttttttga aacgaagcgt cttgaacgtg cagggtaatt cttaaacaat tttgacgatc
     3181 atgccgtttg acgaaaaata tttttccacg catacatatt ccgacattac ctttgccaaa
     3241 tacagcatgt attggtggtc aaatcgtttc tttgctctac tcgcacggcg ctatgggaaa
     3301 cgaggtgcac gcctgctgga aattggttca ggtctgggac atttagtcgg gcagcttgaa
     3361 gatacatttg acacgtatgg aatggatgta aattattggg ctgtgaacca atcgaaatcc
     3421 gtcgtccatg agtcggcttt gcaagccgca agtgcccagg aacttcccta caaagataac
     3481 tcttttaatg ttgtaattat taagcatatt gtggagcatt tgcccgatcc caaaaaagca
     3541 gtggcagaga ttgggcgagt cacagaaaag ggcggcacct tgatcctcgc tacgccaaac
     3601 ctcgattccc tgctcaagcc gtggaaggga gagaagtgga ttggctacca ggatccaaca
     3661 catatctcat tgaagaaacc tgctgaatgg atctccttta ttaaggaggc aggctttgag
     3721 attcaacgtg tgtttgcaga tggattctgg gacgtgccct atattcccgt cgttccaaaa
     3781 caaattcaaa agctgacctt tggctcgctc ggtggatttc aagcgattac aggctttgtc
     3841 ttcctgccga tgaaatgggg cgaaagcatc atcgtcattg cgagcaaaag atagaaacat
     3901 atgagcaacg aactccaaaa caataaccaa ttggttgagc ctagcgttct cgattatgtt
     3961 aagtccctgt ttcggttagg aaatggcgag cggattcaaa ttcccgattt cgtcagagag
     4021 gatcaggagc agcctaacct tcaggaagca agtcatcaac ctaaggtttc ctttccctgg
     4081 cgttccctgc tagcgctgct actggcattg atcggccaaa ctacattcga gccgcctgcc
     4141 aatagcgcgc cgctgggagt tgctttttat gttgcagcgc tgagtatgct gggttgggct
     4201 gtctaccggg gagagtggac cctggcacct caagcccaaa catccgaaca cacagatcca
     4261 cttacatatc ggcttctacc gttatttttt agtgtagtgc ttggcctatg ggcgtttgtt
     4321 attttaggga acaacttctt taccagaaca aacgtgatca tatgggggtt ggccgttgct
     4381 ttttttgtag ggtcattatg gttgaaatca ggaactgggg aatctgtatt ttatagggtg
     4441 ggaaactttc tcaaccgtga ttcctggacg gttaacatct cacgctggac gctgctcctg
     4501 cttgcagcca cagtcgttat attcttcttc cgcttttatc aaacaaccag cgtgccttct
     4561 gaaccattta gtgatcacgc cgaaaagatc cttgatgtgt atgaagtgtc acaagggcaa
     4621 acacatatct tttttattcg caatacgggc cgagaagcgt ttcagatgta ctggactgtg
     4681 ttgatcgcca atctctttgg aacaggcttt tcgtttctca gcctcaaact cggaacagcc
     4741 atcctgggct ttttgacgct gcctttcatt tacctcctgg ggaaagagat cggtggaccg
     4801 cgcgttggtc tgctggcatt tttcctggtg gggattgcct actggcccaa cgtgatcagc
     4861 cgggttgggc ttcgctttcc gctttaccct ctcttcgttg ctccaacact cttcttcctt
     4921 attcgcggcc tgcggactcg caaccggaat gatttcctcc tttcgggact cttcttgggg
     4981 attggcttgc atggctacag tccattccgt attgtaccta ttgtagttgc tgctgctttc
     5041 atcctgtatt ggcttcattc acagtccaaa ggcgctcgcc gtgagttatt tgtttggctt
     5101 gcaattgtcg cattgacatc tttatttgtt tttctgccgc tcctgcgata ttggtatgat
     5161 gatccgacta tgtttggttt tcgtgcgatc acccgagtca gtggtctcga acaacctttg
     5221 ccgggtcctg cacatgagat cttcttctca aatctatgga atgcgctgaa gatgttcaac
     5281 gtggatgatg gggagatatg ggtacattct gttcccgggc gcccggcctt ggacgtggtc
     5341 tccgccgccc ttttcctgtt gggtgtggta ttactcctca ttcgatacat ccgtactcgt
     5401 cactggttgg atctgttcct gctcatttcg attcctttgc tgttgatgcc atccattctt
     5461 tcgctggcgt ttcctggtga aaatcctgcg ctcaaccgtg caggaggtgc ttacatcccc
     5521 gcctttatga tcgcagcatt ggcgctggat ggcctgctca ccaccttagg gcggggaggg
     5581 atacgaacgg tcatgacgtg gatcctcgcg ggagttctgc tgtacgtcgc ggcagaccga
     5641 aactatgagc tggtcttcga tcaatatgcg acacagttca ggcaggcatc ctggaatacg
     5701 tctgacatgg gcaaaatcgt gaaagagttt gagcaaacct atggcaggac ggacaccata
     5761 tggattgtgc catttgaaca ctgggtcgat acgcgtctgc cagccgtctg ggcgggcatc
     5821 ccagatcggg atatggccat ctggccggag aacctgccag gcacggtaga tttgacaggg
     5881 ccaaagttgt ttatggtgaa ggctgagctg gaaaacccgg atctgaacga tcaagcgtct
     5941 ctggacatcc tgcaagagtt atatccaaat ggaaatctgc gaatgtttga ttcagatatc
     6001 gctggtcatg atttctggat tttcacagta ccgcaatagt tgaatttatg aatcgagata
     6061 agtacttctg gctttttgat attttatttc tgctcgtgct tgcactggca ggttatttgc
     6121 ggctgaccgg cgtggcctgg ggtgaaggtc agcaccagca tcccgacgag aatcatttca
     6181 gcagcgtgct ggaaaacctg cgcgcccgtg aatgcgctga gccggcaatt ccgattgaag
     6241 cttgtcctga agaacaaaaa cgatggatca gcattgggga ttactttaat tccaaaacat
     6301 ccactctgaa ccaatataat cgcggcttct ctttttacgt ctatggcaat ctgcccatga
     6361 caattacccg ggttgccgca gaggccctga acacaacgaa tttcaggctt ttcggcaggc
     6421 agatctcggc gctcgcggat ttgttcgcaa tcgttttctt gtatctcagt gtttcgcggc
     6481 tatatggtcg ccgtattggg ctcctggcgg cgttgttctc agcattgact gtaatgcaaa
     6541 tccagcagtc ccatttcttc acggtcgatc tatttgtcaa cgcgtttgct tttttggcga
     6601 tttactttgc tgtgttaatt ttggattaca gacagaagga aatacggact actcaggtag
     6661 atgcatttac ctctagtgag atggcaaacg accctgagcc tgttgtttca aaaacaccaa
     6721 tttcctcatc aagtatagat ctagcaaatc tcaagggatt gattacccat ccgcttttgc
     6781 tgctcagcct gggctttgga tttgcttatg gcatggcact ggcatctaaa gtcaatatct
     6841 atcccttagc aatccttttg ccaggggcgc ttggtttacg ctacctgatt gctaacaaag
     6901 aagccaccga caatggctcc ggagaaagca ccacaaatta ctggtcgtgg gtgattgcat
     6961 gtcttatcgc aggtggattc gctgcgttcg tctccttccg catcttccag ccatacgcct
     7021 tcgatggcct attgcccagc gaacaatgga ttgcaaatat tcaggaacag cgcgctcagg
     7081 caaagggaga tgctgatctt ccctggaacc tgcaatgggc gcgtcgatcc catttctatt
     7141 cttttgagaa tctaaccaca tggggattgg ggctgccgct gggaatcctt gcctgggcag
     7201 gcttccttta tatgggctgg cgtatcctca aaggggagtg gcgccacgcc ttgctgtggg
     7261 gttggacggg agcttatttt gcctggcagt cgcttcagtt caacccaacg atgcgctatc
     7321 aattacccat ctatccgttg ttggcgatga tggcggcctg ggtgatattt gaaatcccac
     7381 gtaattttca atcggagaaa gcgcgttcgg tcgtgcgcag tctttcgtat gtccttggtg
     7441 taatcgtact cgttctaact gccgtgtggg cgtttgcctt tcaaagtatc tatattcgca
     7501 gtgaaccgcg cattgcggct tcacgctgga ttttccaaaa tattcctggc cctatcaata
     7561 ttcaaatcca ggcggaagac tcaatctaca atcagccgct gccgttcccc tctggcgttc
     7621 ggattgattc aacgatgcca taccagacgt ccttcattgc acaaagtgat ggtcttgtaa
     7681 atgaaattct gtttgctcac attgccgcac aaaatcaggc ttctccagag ttgtatctga
     7741 ctctatggca ggacttgaac gacccacaac cactggcttc agcttttaca acaatttcat
     7801 ccgaaaacgg gtttgctggc actggtcaag tagccaattt tgaccaatcg cccctcttaa
     7861 cagcaaatca aatttactat ttgaagttgg agccgagtgt ccttgaaggc acggtagatg
     7921 tttgcggtcc ggtaaatatt caggttcagg cagttgatca attagtagag caggttgtag
     7981 atgtctcgga gccttgtacc gtcacagctg atacccccta tattgcaacc tttattccac
     8041 aagtaactgg cacactaaat accattctat tggaacacgc cacaaattta agtttgaatg
     8101 aagcggttga tcccattacg cttaatctgg ttgtttcaaa agatcctagt gtaactgcag
     8161 aatcgtcaat tgccagaggt tcgctaacag aacagttttc cacttccggt gacccgcgcg
     8221 gtggttcata taccctgact cttgaccagc ctgtaaaagt ggaaaaagat acacaatatt
     8281 atcttcaact tgaagttgac tctggtactc ttggtctgag cggcgcttct atttctaatg
     8341 agacagatta cgattatgga cttccgttcc gccttgacgg ctatgacgcg tttggcggca
     8401 tctatcgtgg cgacctcaac cttcaagtct actgggatga caatgcggat aagctcaacc
     8461 gctttgtaac aacactcgat cagacggatt acatcttcat cccaacaaat catcaatacg
     8521 cacagatcac acgccttcca gagcggtatc ctctcacaac actttattat cgcgagttga
     8581 tgggctgccc ggaggataaa gagatcatct ggtgttatca caccgcaaaa ccggaccagt
     8641 tcaaaggtcg gctcggattt gatctcgttg ccgttttcga aacgtatccc acactgggcc
     8701 caatcgtgat caatgatcaa tgggctgagg aggcctttac tttctacgat catccaaaag
     8761 tgttgatctt taagaagagt gagaatttta ataaggaaca ggtagggtcg attctgagca
     8821 cagtggatct ggcgaatgtc gtccggctca ccccgcctca attcagcgat tactccaatt
     8881 tgctattatc tgcagaaaaa ctcgctcaac aacgcgcggg tggtacgtgg tctgatttat
     8941 acagttatga gtggctgcaa aatcgctatc ctattttagg attgttgatc tggtatttgt
     9001 ttatctttgt ccttggactc gcagtttatc cattaattcg gctgacgatg cccgggctgg
     9061 cagataaggg ataccccctc agtcgcgcct tgggattggt gctttttggg tatctggcgt
     9121 gggtggctgg ctcaatcggt atcccataca cgcgcctttc cattacaggg acgtttggtc
     9181 tgattctcgg cgtgggaatc ctgcttgcaa gatatcagcg cgatgagatc cgtgaagaat
     9241 ggagaaacaa tcgcaagtat ttcctcatgg cggagggaat tttcctgacc ttcttcctga
     9301 tcgatttgct gatccgtttt ggtaattcag atttgtggca tcccgccaaa ggtggcgagc
     9361 gcccgatgga tttctcttat ttcaacgccg ttctgaagag cacctccttc ccaccctatg
     9421 atccctggtt tgcgggtgga tacataaatt attactatta tgggtttgta ctggtcgcca
     9481 caccagtgaa gttactgggt atcgtgcctt ccatcgccta caactttatc ttgcccactt
     9541 tatttgcgat tgtggga
//
LOCUS       DASTJP010000677         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1218407, whole genome shotgun sequence.
ACCESSION   DASTJP010000677 DASTJP010000000
VERSION     DASTJP010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218407"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..909)
                     /locus_tag="VFQ23_19775"
     CDS             complement(256..909)
                     /locus_tag="VFQ23_19775"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HET9908900.1"
                     /translation="MKTNRLSRLKIISVIGIAALALVGFRQMFGQYSISRLQQAPLNL
                     DVPLVKQLTGTSCGEAVIAMAYNYAYPETPLQEQEVIDYATAQGYFTPGVPPFTSPAN
                     MVNIAENYVDDVAAGTVKNSSEGLSLLIRRLREGDPVIIDVLSNFSDPESEAHFILVT
                     GISVDPNRGNAIVIEYNDPLTGTKKSGDWYGTEGVWNAWQNNGDPGGPGWWMVIPVR"
     gene            complement(1095..1355)
                     /locus_tag="VFQ23_19780"
     CDS             complement(1095..1355)
                     /locus_tag="VFQ23_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908901.1"
                     /translation="MAKLIPATERIIRARKLIQEARDYPVPQGGLGKSDFSYIAQIKD
                     LLRQARDMVKFIPQTAGVSVEMKEEVKRIYEEADQANQDILY"
     gene            complement(1502..1858)
                     /locus_tag="VFQ23_19785"
     CDS             complement(1502..1858)
                     /locus_tag="VFQ23_19785"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9908902.1"
                     /translation="MKTNHEFENIERVSNTKSVLAGILLGGLVGAGTMLLLAPQAGQR
                     TRAELQDGVSHLRDRTTETVKDKVTLVKSRANQIKSDVQVKAGELQHQGKDLLAKQLD
                     RVSHAAEAGKKAIQES"
     gene            complement(2195..2452)
                     /locus_tag="VFQ23_19790"
     CDS             complement(2195..2452)
                     /locus_tag="VFQ23_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908903.1"
                     /translation="MEATANRSDALELNEMMARPFRRAVDLIHVITEDFPTYYGSQNL
                     EIETLNTPPRGTTQIVGGPKVTGATCGPSRLIALICRGDDD"
     gene            2719..3201
                     /locus_tag="VFQ23_19795"
     CDS             2719..3201
                     /locus_tag="VFQ23_19795"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HET9908904.1"
                     /translation="MNNQNILHAFLGEWSGTGRGEFPTIEPFDYLETLSFATDGRAFL
                     HYEQKTQRHRAGEVDYVPSHWESGFIRLLADGQLELTCAQNGGRLEGLSGSIEETKQG
                     LILRLTRADFLNDARMLGTSRTIAIQGDLLHYIQTMHTTAVPRPTQHLEATLHRRENS
                     "
     gene            3201..>3845
                     /locus_tag="VFQ23_19800"
     CDS             3201..>3845
                     /locus_tag="VFQ23_19800"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9908905.1"
                     /translation="MNYTDILRAFGFEIEADATPHSIYDYAPVFRVSNSDGSWIVKRT
                     QPPLARAQAIVAWTQSLAALGISVVNPAPGFGENPRAFLNKDGKEQPWVVYPFIDGQP
                     YEGDRTQIRAAGELLGRIHAAGSDQDFGLKQRETVIIIEAEEVGGDIERVLGFMQQAF
                     PHLVDEASVHLQQQAQHYFQHTAPRMTSAALPVTNCSWDYKASNLIFQPTGPVLI"
BASE COUNT          952 a          967 c          895 g         1031 t
ORIGIN      
        1 aatggtcata tccccgcctc ggaccaacat cattctcccg cctctcatca aaaacatatc
       61 tggtttcatt tacatgctcc ttgtttatga atggaccaag cccaaaaaag aatggcttta
      121 cgttgcaaat cattgtatga acagggaaat caaacgtcaa cggagaatgt ccctacctgc
      181 agaatagcac tttcatcccc aagagctttg ggatatctgt aaatttgcct attgagaatt
      241 aaagatgaaa tttgattacc gaaccggaat caccatccac cagccgggcc cgcctgggtc
      301 tccattgttc tgccaggcgt tccaaacgcc ttccgttcca taccagtcac ctgatttttt
      361 tgttccggta agcggatcgt tgtattcgat gacgatggca tttcctcggt tcgggtcaac
      421 cgaaataccg gtcacaagaa tgaaatgtgc ctcggattcc ggatcactaa aatttgaaag
      481 cacatcaatg atcactgggt caccctctcg caatcgtcgg atcaacaagg acaatccctc
      541 actggaattt tttacagttc cagcagcaac gtcatcgaca taattttcgg cgatattcac
      601 catattcgcc ggactcgtaa aaggtggaac tcctggagtg aagtatccct gcgctgtcgc
      661 ataatcgatt acttcctgct cctgcagtgg agtttctggg tatgcgtagt tatatgccat
      721 tgcaataacc gcttcaccac aggaggttcc ggtgagctgt ttcaccaggg ggacgtccaa
      781 attcaacgga gcctgttgta gccgtgagat tgaatattgg ccaaacattt ggcggaaacc
      841 tacgagtgcg agtgccgcaa taccgatcac actgatgatt ttcaagcgtg ataatctatt
      901 cgttttcata aatatttagc tctctgagat ggtttaacga ttcatctatg attctgacgc
      961 ggctttgcag cattcccttc aggaaacgcg ataaatatcg aattagagtt tgctaagatg
     1021 ggattaattt atgatgttct tcaaatcatt tgccccacag gaatagtgtc taatctcacc
     1081 tgaaatcctt tcccttagta caggatatcc tggtttgcct ggtcggcttc ttcatatatt
     1141 cttttgacct cttctttcat ctcaacagaa acgccggcag tctgcggaat gaatttaacc
     1201 atgtcccgcg cctgacgtaa gaggtccttg atctgcgcaa tataggagaa atcacttttt
     1261 cccagtccac cttgtgggac agggtaatcc cgagcttctt gaatcagctt acgggcgcgg
     1321 atgatacgct ctgttgcagg gattagtttt gccatgatca ctcctgtttg aggtgagaaa
     1381 gtccgcaata attatacttt ctggatgcct cacttttact ccgttgataa tatttaaaaa
     1441 acaaacccct tcccattgat gatgagaagg ggtgcacgaa acacttgctg ctgtgtagcg
     1501 ctcaggattc ctgaattgct ttcttgccgg cctccgccgc atgcgacacg cgatccaatt
     1561 gcttggcaag gagatccttg ccctgatgtt gaagttctcc ggctttcacc tggacatcag
     1621 atttgatctg attggcgcgg gatttaacca gcgttacctt atctttgaca gtctcagtgg
     1681 tgcggtcacg caaatgcgac actccatctt gaagttcggc ccgcgttcgt tgaccggctt
     1741 gtggtgccaa gagtagcatg gtgccggccc caaccagccc acccagcaat atcccagcca
     1801 gaaccgattt ggtattgctt acgcgctcta tgttttcaaa ttcatgattg gttttcatta
     1861 ttttctcctt cttgtgggca gatttaatct gccgtgatct atacgaattc gcaggacaat
     1921 atttcaaata cgaaccgcgt aagtagagag tttctaatat atctaacgat ggttaattta
     1981 tgttgttatt ttggattgta taaataaatg tacgactcga tccttaattc cgcatgaaag
     2041 ttcgtacatt aatataggtt tctcaaaaaa ctttaatctt acgaagaacg gattacacaa
     2101 agaggagcga cctctttgtg tgtcgctcct ctttgttatt tctggagaat ctccatactt
     2161 gtctttgtga gcgcagcgca ttccgtcagc gatctcaatc gtcgtcgccc cggcagatca
     2221 gggcgatcag gcggctgggt ccgcaggtgg cgccagtgac tttgggtccg ccgacgatct
     2281 gcgtcgtgcc gcgcgggggt gtgtttaacg tctcgatctc caggttctga gatccgtaat
     2341 aggttgggaa atcctcggtg atcacatgga tcaggtcaac cgcacgccta aagggccgtg
     2401 ccatcatctc gtttagttcc agcgcgtcgc tacgattcgc tgtggcttcc aaagccgcgg
     2461 gcgacaccat tgcgccggca acaagcgcag ctgtcgcctt gaaaaaatca cggcgcgaca
     2521 gccagccgct tcataactac ttcctgagtg ccgggtacgc aaatagggat actcctttcg
     2581 tatgtagttg aaaaaagggg aacgatattt tataaatcat actcatcttt aattcggtgt
     2641 caatcaactc tatgcttacg aaaggcgcat ataattacct tccacggcgc gctatttcgc
     2701 tccttcaaaa ggataaagat gaacaatcaa aatatcctgc atgcattcct cggcgaatgg
     2761 tcggggacag gtcgcgggga atttcccact atcgaaccgt ttgactatct tgagaccttg
     2821 agctttgcca cagacggacg tgcgttcctg cattatgaac agaagacgca acgccaccgc
     2881 gctggggaag ttgattatgt cccttcccat tgggaaagtg ggtttatccg tttattagct
     2941 gatgggcaac ttgaactgac ctgcgcccaa aacggtgggc gacttgaagg actcagcggc
     3001 tcgattgaag aaacaaagca aggattgatc ctgcgtctaa cgcgtgcaga ttttttgaat
     3061 gatgcccgca tgttggggac atcacgaacg atcgcgatcc agggagattt attgcattac
     3121 attcagacca tgcacacaac ggctgtgcct cgaccaacgc aacacctgga agccaccctt
     3181 caccgaagag agaattcctg atgaattaca ctgacatcct tcgcgccttc gggtttgaaa
     3241 tcgaggccga tgccacgcca cacagcattt atgactatgc ccctgtgttt cgggtctcca
     3301 attctgacgg ctcatggatt gtcaaacgca cccagccacc gcttgcgcga gcccaggcaa
     3361 ttgtggcatg gacgcagtca ctcgcggcgc ttggtatttc ggttgtaaac cccgcgcctg
     3421 gctttggtga aaaccctcgc gcattcctga acaaggatgg caaggaacag ccctgggttg
     3481 tttatccgtt catcgatgga cagccttatg aaggcgatcg tacgcaaata cgcgcggccg
     3541 gagaattgct tggcagaatc catgcagccg gatcggatca ggatttcgga ctcaaacagc
     3601 gtgaaaccgt tatcatcatc gaagccgaag aggtgggagg agatatcgaa agagttttgg
     3661 gattcatgca gcaggctttt cctcacctcg ttgacgaggc aagtgtacat ctacaacagc
     3721 aggcacagca ctattttcaa cacactgcgc cgcgcatgac atcagcggct ttaccagtga
     3781 ccaattgcag ctgggattac aaagcttcca atctcatctt tcagccaacg ggcccagtgt
     3841 tgatc
//
LOCUS       DASTJP010000678        11652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1324852, whole genome shotgun sequence.
ACCESSION   DASTJP010000678 DASTJP010000000
VERSION     DASTJP010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324852"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1667)
                     /locus_tag="VFQ23_19805"
     CDS             complement(6..1667)
                     /locus_tag="VFQ23_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908906.1"
                     /translation="MYDTPFYRKPWFYIFAWLAILLVVYGWQIFRMGGVQAGLIDILI
                     DLVFVFPILLLLWIAFFAQFVLPVRTFRDRRKIVSRLLTDLFGGHGPALFIENGIIKE
                     HSGERLKSGPGVLWLDSASAAVTRSATAIRQTMGPGVHFMQSGEFIAGTVDLHIQSQS
                     IGPKDSDAPFASKSDDQSDEEYHQVQDRRKQVSALTRDGIEVVPNISVSFRVDTGFPA
                     PGQPGSRFGYRTGVTKKAKMEEQKDKDAIRKAILGEGINPNIQPGSPRHRVAWNQLPA
                     ALAVDVWREYVAKFTLDELFAPDQLVPAPPFQPPQPTEEEIDFLSQPIQVGARSESMQ
                     ENMQVSMTRMLRQVNKMMDRAIKWLERTDKGPKNQTPAPAVPTGQSPRKSEPQKKTGL
                     QVINEMVKARLTQAEVEILDDNGARGNGMISSAEYKLLQERGLRVLNASVVGLRLNPT
                     IEETLINRWSASWLVNARVEKEQIERKRNIFESAGQERAIRQYADLFSRDLIQKKPIG
                     VKDTLKTLLLRTRTIIFNNDQLRKRMASQQQELEDIIKWIEVSGS"
     gene            1744..2400
                     /locus_tag="VFQ23_19810"
     CDS             1744..2400
                     /locus_tag="VFQ23_19810"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9908907.1"
                     /translation="MLKVEFHCHTSASGDSLTRPRALVDTCRRKGIDRVIVTDHNSIE
                     GARAAYQIDPELVIVGEEIATTRGEILAAFVTEEIPKGLSPAETIRRLKNQGAFISVS
                     HPFDEWRSGGWREEDLLEILPEVDAIEVYNSRCMLPRFNREARTFAEKHNIPGTVGSD
                     AHAAFELGRSLIILEPFAGPDELRKVIRAGFPLVRWSPPWFHVTSRYATIRKKLAAKH
                     "
     gene            2577..2789
                     /locus_tag="VFQ23_19815"
     CDS             2577..2789
                     /locus_tag="VFQ23_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET9908908.1"
                     /translation="MSERYVGTVKWFNATKGYGFIGRDGGEDVFVHFSAIQTDGYRKL
                     EAEQKVEFSVEDGPKGLQAANVVPVS"
     gene            3221..4531
                     /locus_tag="VFQ23_19820"
     CDS             3221..4531
                     /locus_tag="VFQ23_19820"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9908909.1"
                     /translation="MNYSIIGMGVAGIAGIEAIRSVDSNGEITLVGDDPHGFYSRPGL
                     AYYLTGELHDQALFPRTREDFQRMNFRHLKGRVASIQREGHLIELDNKTQIPYDKLLI
                     AVGAQATSMNVPGASLDGVLKLDHMEDANRILKHARRGRTAVVVGGGITALELVEGLL
                     SRGVKVHYLLRGERYWSNVLDEQESKIVEHRLKEEGVRLHYHSEIGEVLGKRDRVSGV
                     RLVSGETIKCDLVAYAIGIRPRLELAHQAGLATDRGILVNEYLQTNDPNIYAAGDVAQ
                     VFDPRSGRSVLDSLWGAAREQGSAAGLNLAGEQKAYLKPVPFNVTRLAGLTTTIIGNV
                     GQGRDSDLIGIARGDSETWRQSPDAMVAQTGFDVNRLRLVVGEKTLLGAIVMGDQRLS
                     FPLEKIITHNADISPIRQQLLMPNAKLGDILSEFWAGIKNQSMS"
     gene            4582..5136
                     /locus_tag="VFQ23_19825"
     CDS             4582..5136
                     /locus_tag="VFQ23_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908910.1"
                     /translation="MLRGYKELWLAAFAILLITGIYVTVVMFTREIPPAGELFGHGMG
                     ILGFVFMLMTETLYSLRKRSRSVKWGKLSSWLQFHIFTGIVGSYMVLLHSSWKFNGIA
                     GITTLFTIIIVISGFTGRYIFTRIPRTLDGLEIEGRLSPEALKQARRLLALWHTIHIP
                     IGMVLFTSAIAHICGALYYATLLR"
     gene            5148..6173
                     /locus_tag="VFQ23_19830"
     CDS             5148..6173
                     /locus_tag="VFQ23_19830"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET9908911.1"
                     /translation="MRQRLGCLSGTGLIAASVTALLIAGYVYARGGLLFNPGPLNAQS
                     GRLLGGVTSHAEIDGDCESCHTAPWESATMADRCVVCHTEIKTELQRAETLHGAIMFN
                     NPSLACRDCHPEHRGPEAPLVFMEDGTFPHEAVGFSLAGHQQTSAGIPFACQDCHQDD
                     ITTFTADTCYACHHQMDIEFAMPHLLTFGPGCLACHDGVDRYGDGVDHNQFVFQLTGS
                     HAAVTCSGCHQNARTIADLQSTPQDCFSCHRQDEPHAGQFGTDCSACHTTSAWTPSQF
                     NGQHTFPLNHGGGATCATCHTAGFTTYTCYGCHEHEEAEVIERHQDKDIPNFQNCMAC
                     HPSGEKD"
     gene            complement(6180..7457)
                     /locus_tag="VFQ23_19835"
     CDS             complement(6180..7457)
                     /locus_tag="VFQ23_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9908912.1"
                     /translation="MQSSTPTIPQNWTRRFFTIWTGQAFSLFGSALVQFALIWWLTQR
                     SGSATVLAIATLVGMLPQIVIGPFAGALVDRWNRRLIMILADALIAVSTLMLVYLFAT
                     DQVQIWHVYAILGMRSLGTAFHFPAMSASTALMVPEDQLTRINGLNQTLLGINGLIAP
                     PVGALLIGILPTHNILLIDVATAILAILPLFFFTIPQPVRDESANASKPSLFQDIREA
                     FNYIRGWSGLVAIISMALFLNFLLVPTGALLPILITKHFEKGALELGLINSAEGVGII
                     AGGILLSVWGGFKKKKIVTSMAGIFLLGISVMLVGVAPANLFALAVAGLFLTGLAIPI
                     TNGPIGALFQSLVRNDMQGRVMSLVNSAATAISPLGLLIAGPLSDALGIQIWFWAGGL
                     ICALIGIAGFFVPSIMNIENNKEEIPTPAPEPG"
     gene            7530..9200
                     /gene="ggt"
                     /locus_tag="VFQ23_19840"
     CDS             7530..9200
                     /gene="ggt"
                     /locus_tag="VFQ23_19840"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9908913.1"
                     /translation="MTNLSFNSRRSPVYGPGGIVSTSQPLATAAGLEILAKGGNAADA
                     AVAAGAALNVTEPTSTGIGGDMFALYFSADTKRVTALNGSGRAPAALTLDRLKSEGFS
                     SELPPYHAHTVTVPGACAGWFDLIERHGSLSMGEILAPAIRLASEGFPVAPLTSKSWS
                     YAVQRQLKTAPNGNELTIDGREPRPGEIFRNPGLARTFETIARGGKSAYYQGDIAEAI
                     VSVMKEAGGTMSAEDLASHVSTWEEPISVTYRGLNVYECPPNGQGITALIALNILEGF
                     DLASLEQLSTERMHLMIEAMRLAFADASWYVADPALANIPVQELLSKEYAEERRNLIN
                     KTRATIDQKHGTPVASSDTVYLSVVDKFGNACSFINSNYMGFGTGIVPKGWGFTLQNR
                     GHNFSLDPNHPNALAPRKRPYHTIIPAMVTRLPSPRGRGMSEGQGEGEETLFASYGVM
                     GGFMQPQGHVQVLSALVDDGLDPQAALDRPRFCVDVEESGGRVAIEEGMPDETYSGLE
                     RMGHPVYRVTSYNRALFGRGQVILRDPETGVLCAGSDPRADGCAMTLI"
     gene            complement(9246..9782)
                     /locus_tag="VFQ23_19845"
     CDS             complement(9246..9782)
                     /locus_tag="VFQ23_19845"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9908914.1"
                     /translation="MKSKFDPKKHHRRSIRLKGYDYSSEGAYYVTIVTYQRDCLFGDI
                     VKKKMILSDFGKIADECWRAIPEHFPFAELGVYVVMPNHVHGIIIIRRGTIYRAPTME
                     QFQKPVAGSIPTIIRTYKAAVTRRIGREHNATGIWQRNYYEHIIRDEKDLQNKTDYIE
                     ANPSLWDEDDNNPRNIQM"
     gene            complement(9843..10466)
                     /locus_tag="VFQ23_19850"
     CDS             complement(9843..10466)
                     /locus_tag="VFQ23_19850"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HET9908915.1"
                     /translation="MKKAIFIGSALGRNVIPIGAFLFGGNSAETALVIYFLETLIAIL
                     LAAVFVRLRAPAEDSGYGKISSTKTVIKTNNSVIRRYQAGNRRSMLESFLLFSIAFAL
                     VPGIFIGVFLFGIQRAEISISVVASAIAGIAAFQIISFIVDLFRVDSVTPESAYASLE
                     QSMGRSAILFLSCFIGIFLAAFVTEWFIVPFAFLKTVTEVGAIVKRD"
     gene            complement(10542..>11652)
                     /locus_tag="VFQ23_19855"
     CDS             complement(10542..>11652)
                     /locus_tag="VFQ23_19855"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HET9908916.1"
                     /translation="LRDRRVSYLGLEILRFAQDDNFIEITTLQNAKSMLEQVASTYAE
                     TIIAAIQQEYPNQMQHMMTGPEDRPRPREAHPAFYGCFDWHSSVHMHWSLIRLLRLAP
                     DAFDNAKAITVLEAHLTKEALAQEVSYLHARPRFERPYGWGWALTLAYELTLWKTKEA
                     AQWLAAVRPLADVITELMLAWLPKATYPIRTGMHSNSAFGLARSVPWARVLAKQGDTR
                     LLTAITRTAIQWYGEDRDYPASYEPSGSDFLSPALTEVELMSVLLEREAFLAWLDAFL
                     PGLPSGHPKSLFEPAFVSDAADGQLAHLHGLNLYRAFVWKHLHAILPADDPRRSYLEA
                     GIESHARASMGAVTGSDYMVEHWLASYALLYLSGG"
BASE COUNT         2801 a         3007 c         3070 g         2774 t
ORIGIN      
        1 ggtggtcatg agccagacac ctcaatccac ttgataatgt cttccagttc ctgttgttga
       61 gacgccattc gtttccgcag ctggtcatta ttgaaaatga tcgtacgcgt ccgcaggagt
      121 agagtcttca gcgtgtcctt cacgccaatg ggtttctttt gaatcagatc tcggctgaat
      181 aagtctgcgt actggcggat ggccctttcc tgccccgcag actcgaagat attccgtttt
      241 cgttcaattt gctctttttc caccctggcg ttaaccagcc aactggctga ccatctgttt
      301 atcagtgttt cctcgatggt cgggttgaga cgaagcccta ctacgcttgc attaagtacc
      361 cgaaggccgc gctcctggag caatttatac tccgcactgg aaatcattcc attgccgcgg
      421 gcgccattgt catcaagaat ttcgacctct gcctgagtaa gccgtgcttt gaccatttcg
      481 ttaatcacct gcaaccctgt tttcttttgt ggctcacttt tccttggtga ttgaccagtt
      541 ggaacggcag gcgcaggcgt ttgattttta gggcctttat cggtccgctc aagccatttg
      601 atggctcgat ccatcatctt attgacctga cgcaacatgc gggtcatgct gacttgcatg
      661 ttttcctgca tactttcact tcgagcaccg acctgtatcg gctgacttaa aaaatcaatc
      721 tcctcttcgg tgggttgtgg cggctgaaaa ggtggagcgg gcacgagctg atctggcgca
      781 aacaactcat ccaaagtgaa cttggcaaca tactcacgcc agacgtctac agccagcgcg
      841 gcgggcagtt gattccaggc gacgcgatga cgtggtgacc caggctggat attcggattg
      901 attccctctc cgaggatcgc tttgcgaatc gcatctttat ctttttgctc ttccattttc
      961 gcttttttgg taacgccagt ccgatatcca aaacgagacc ccggttgacc gggtgctgga
     1021 aatcccgtgt cgactctgaa tgagacggaa atatttggaa ccacctcaat tccatcgcgc
     1081 gtcagagcgc tgacttgttt gcggcggtcc tgaacctggt gatattcctc atcgctctga
     1141 tcatcagatt tggacgcaaa tggcgcatca gaatctttcg gtccgatgga ctgggattga
     1201 atgtgcaagt ccacggtccc ggcgataaac tcaccgcttt gcatgaaatg cacacccggg
     1261 cccatggtct ggcggattgc ggtcgcagag cgcgtaacgg ccgcgctggc ggagtcaagc
     1321 cacaacacgc cgggtccgct tttgagccgt tcgccggaat gttccttgat aatgccgttt
     1381 tcaataaaca gggccggacc atgcccgcca aagagatcag tgagtagcct gctgacgatc
     1441 ttccgacgat ctcggaacgt gcgaaccggc aggacgaact gcgcgaagaa agcaatccaa
     1501 agcaataaaa gtatcggaaa aacaaaaaca agatcgataa ggatatcgat taaccccgcc
     1561 tgcacacctc ccatgcggaa aatttgccag ccatacacca ccagcaaaat cgcgagccag
     1621 gcgaaaatgt aaaaccacgg cttcctgtaa aagggagtgt cgtacatatc ttggattatt
     1681 ataacaaaat cgtatcattt gcccgcggtc aatcccggga agagtgagag tacaatacct
     1741 tacatgttga aggttgaatt tcattgccac accagtgctt caggcgattc cctgacgcgc
     1801 ccgcgtgctt tagtagacac ctgccgacgc aagggaattg atcgcgtcat cgtcactgac
     1861 cataactcga tagaaggtgc gcgcgcggct tatcagatcg accctgaact tgtcattgtg
     1921 ggggaggaga tcgcgaccac ccggggagaa atcctggccg ccttcgtcac agaggagatt
     1981 ccaaagggac tttcgcctgc agaaacaatt cgccggctca aaaatcaagg cgcgttcatc
     2041 agcgtgtccc atccctttga tgagtggcgt tcaggcggct ggcgggaaga ggatctgctt
     2101 gagatcctgc ccgaggtgga tgcgatcgag gtctataact ctcgctgtat gctgccgcgg
     2161 ttcaatcgtg aggcacgcac gtttgccgaa aaacataata tccctggaac cgtcggttcg
     2221 gacgcgcacg ccgcgtttga gttaggcagg tcgctgataa ttttggagcc gtttgcaggt
     2281 ccggatgagt tgcgaaaggt gattcgagcg ggttttccgc tggtaaggtg gtccccaccc
     2341 tggttccacg taacttcgcg atatgcaacg attcgcaaaa aactggctgc gaaacattaa
     2401 catcaccgtg taactccgag ttcagattag aaaaagtaac aatctatcgc atatggcaaa
     2461 cttggtcttg acactaaatt tcagccatga taaccttgcc tgcgtcgtgc ccgattcgcc
     2521 ttcgaaatcc gcaaaggcgg tcgggcaaaa attaactctc agcaggagat tgtaagatgt
     2581 cagaacgata tgttggaacc gtcaagtggt ttaacgccac gaagggctat ggcttcatcg
     2641 gtcgcgatgg tggagaggac gttttcgtcc acttcagcgc gatccagact gatggctacc
     2701 gcaagttgga agccgaacag aaggtggagt tttcggtaga ggatggccca aaaggtttac
     2761 aggctgcaaa cgtagtgcct gtatcgtaag actctgcctg ctgcccgctg gctcaagttt
     2821 tgcgacccca acagagttgc cgtgaggtaa ctctgttttt ttgtttttcg tcatttcgaa
     2881 tgttaggaat attgggggag cagaagactg acccatgccc ataacttttc gccggattca
     2941 acgactatat taatattatg agactattgg atttcttgaa atcagacacc gataagcata
     3001 ctgttcttgc ttccggatat gtcgttcccg attccacacg ctgcgtccaa tgcgggatct
     3061 gtacctataa ctgcccaatt ggcattgatg ttcgtggcca cgcctggcgc ggggagccag
     3121 tgattaacag taactgtttg acatgcggcg aatgtgtaaa gcgctgcccg cgcggagtac
     3181 tgcgcttcga acagacggat ctgttcgcca aaggcactga atgaattact ccatcattgg
     3241 gatgggtgtt gccgggatcg cggggatcga ggcaattcgt tctgtagatt ccaatgggga
     3301 aatcaccctg gttggtgatg acccccatgg attctattca cggccagggc tggcttatta
     3361 cctgacgggg gaactgcatg accaggctct cttccctcgc acacgtgaag acttccagcg
     3421 gatgaacttt cgtcatctaa aaggtcgagt tgccagcatc cagcgagaag gtcatttgat
     3481 tgaactggat aataaaacac agattccgta tgacaaattg ttaatagccg taggcgcgca
     3541 agccacttcg atgaatgtcc cgggagccag tctggatggt gtcttaaagt tggatcatat
     3601 ggaagacgcc aaccgtatcc tgaaacatgc gcggcgcggg cggacagcgg tggtagtggg
     3661 tggaggaatc acggccctgg agttggtaga agggcttctc tcgcgcgggg taaaggttca
     3721 ttacctgttg cgtggagagc gttattggag caacgtcctg gatgaacaag agtcaaagat
     3781 tgtagagcat cgcttgaaag aagaaggcgt gcgcttgcat tatcactctg agattggcga
     3841 agtacttggt aaacgtgatc gggtcagcgg agtgcggtta gtgagtggtg aaaccataaa
     3901 atgcgatctg gtagcttatg caattgggat tcgcccgcgc cttgagctgg cgcatcaggc
     3961 cgggctagcc acggatcgcg gaatattggt caatgagtac ctgcagacca atgatccaaa
     4021 tatttacgcg gccggcgatg tggctcaagt ctttgacccg cgttcggggc gatctgtttt
     4081 ggatagttta tggggagcgg ctcgagaaca aggatctgcc gccgggctga atttggctgg
     4141 cgagcaaaaa gcatatctca aacccgtgcc ctttaatgtg actaggttgg ccgggctaac
     4201 cacaaccatt atcggtaacg tcgggcaggg ccgcgacagt gatctgatcg ggattgcgcg
     4261 cggcgatagc gaaacctggc ggcaatcgcc ggatgccatg gtcgcgcaga ccggctttga
     4321 cgtgaatcgc ctgcgcctgg tggtcggtga aaagacgctg ctcggcgcga tcgtcatggg
     4381 cgatcagcga ttgtcttttc cattagaaaa aataattacg cacaatgcag atatttcccc
     4441 tattcgacag cagttactga tgcccaatgc aaagctcgga gatatccttt ctgagttttg
     4501 ggctgggatt aaaaatcagt ccatgtcttg agttctattg cttctttcgc tttcaaagtg
     4561 cgctaacgtg gtgaaacgat aatgctccgc gggtacaaag aattgtggct ggcagctttc
     4621 gccatcctgt taataacggg catttatgtg accgttgtta tgttcacgcg cgagatcccg
     4681 ccagcgggcg aattgttcgg tcacggtatg ggaatcctcg gatttgtttt catgctcatg
     4741 acggagaccc tttatagcct ccgcaagcgc agccggagcg tgaaatgggg aaagctatcg
     4801 agctggcttc agttccacat ttttacaggc atcgtcgggt cctatatggt attgctgcac
     4861 tcctcgtgga agttcaacgg cattgcaggg attactaccc tgttcacgat cattatcgtc
     4921 attagcgggt tcacgggaag atacatcttt acccgcatcc cgcgcacgct ggacgggctg
     4981 gaaatcgaag gaaggctttc accggaagct ctcaaacagg cacggcgatt gctggcgctt
     5041 tggcatacga tccatattcc gattggcatg gttttgttca cgtccgcaat tgctcatatt
     5101 tgtggggcgt tgtactatgc tactcttttg aggtaaagca gaaacgaatg cgccagaggt
     5161 taggttgctt atcaggaact ggattgatcg ccgcgtcggt cacggctctc ctcatcgcgg
     5221 gctatgtgta cgcgcgcggc ggactgttat ttaacccagg tccgttgaac gcacaaagcg
     5281 gaagattgtt gggcggagtc acgtctcatg cagagattga cggagactgc gagtcgtgtc
     5341 acaccgcgcc ctgggaatca gcgacgatgg cagatcgttg tgtggtctgt catacagaaa
     5401 tcaagacgga attacagcgt gcagaaacgt tgcatggcgc aatcatgttc aataatccga
     5461 gccttgcttg tcgcgattgt catcccgaac atcgcggacc cgaagctccg ctcgtgttta
     5521 tggaggatgg cactttccca catgaagcag tggggttttc actcgccggg catcaacaga
     5581 cttctgcagg cattccgttt gcctgtcagg actgtcatca agatgacatc acgaccttta
     5641 cggcggatac ctgttatgcc tgtcaccatc aaatggatat agaatttgcc atgccccatc
     5701 tattaacgtt tggacctggc tgtctcgcgt gtcacgatgg tgtcgatcgt tacggagacg
     5761 gcgtcgatca caaccagttt gtctttcagt tgacaggcag ccatgcagct gtgacctgct
     5821 caggctgtca tcagaatgcg cggacgatcg cggatttaca atccacgcct caggactgtt
     5881 tttcctgtca cagacaggat gagccgcacg caggtcaatt cggaacggat tgttcggcct
     5941 gtcatactac ctctgcatgg accccttctc aattcaatgg acaacacacc ttcccattaa
     6001 atcatggcgg cggcgctacc tgtgcgacgt gtcatacggc tggttttacg acctatactt
     6061 gttatggctg tcatgaacat gaagaggcgg aagtgattga aaggcaccag gataaagata
     6121 ttcctaattt ccagaattgt atggcgtgtc acccctcggg cgagaaagat tgattaattc
     6181 tagccgggtt ccggcgcggg cgtagggatt tcctctttgt tgttctcaat attcataatg
     6241 gatgggacaa agaatccggc aatcccaatc agagcgcaaa tcaagccacc tgcccaaaac
     6301 cagatttgaa tgccgagcgc gtccgagagg ggaccggcaa tgagcagtcc aagtggggaa
     6361 atcgcagtcg ctgcgctgtt caccagagac atcacgcgtc cctgcatatc gttgcggacc
     6421 agagattgaa acagggcgcc gatcggtcca ttggtgatgg ggatggccag ccctgtgaga
     6481 aaaagccccg cgacggccaa agcgaacaaa ttggcgggag ccactccgac cagcattact
     6541 gagattccaa gaaggaagat ccccgccatg gaggtaacta tttttttttt cttgaagccg
     6601 ccccacacgc tgagcaggat tccacccgcg atgatgccca ctccttccgc ggagttgatc
     6661 aacccaagtt ccaatgctcc cttctcaaag tgcttggtga taaggatggg gagtaaggct
     6721 ccagtgggta ctagcagaaa gttcaggaag agagccatgg aaataatggc gaccaggcca
     6781 gaccagccgc gaatgtaatt aaacgcttcg cgaatgtctt ggaacaggct tggctttgat
     6841 gcattggctg attcatcgcg tactggctgg gggattgtga aaaagaataa aggcaggatc
     6901 gcaagaatcg cagtagcgac atcgatcaac aaaatgttat gggttggcaa aatgccaatc
     6961 agcaaagcac ccacgggtgg cgcgatcagg ccattgattc ccaaaagcgt ctgattgagt
     7021 ccgttgatgc gcgtgagttg atcctctggc accatcaacg ctgtggaagc agacatcgcc
     7081 gggaagtgaa acgccgtgcc taacgagcgc atacccagaa tagcatatac atgccagatc
     7141 tgcacctggt ctgtagcaaa taaataaaca agcataagag tggaaacagc gatcagtgca
     7201 tctgccagga tcatgatgag gcggcgattc cagcgatcga cgagcgcgcc ggcaaagggt
     7261 ccaatcacga tctgaggcag catgcctacg agagtcgcaa tcgcgaggac cgttgctgaa
     7321 ccgcttcgtt gagtcagcca ccagatgagc gcgaattgaa cgagcgcaga accgaacagc
     7381 gagaaggcct gcccggtcca aatcgtgaag aaacgccttg tccaattttg aggaatggtt
     7441 ggtgttgaac tttgcatgat cgtccgttct gtgttggttg tcgctatata atatacgaca
     7501 taaatattcc aaccgttcaa ggattataca tgacaaacct ttccttcaat tcacgccgtt
     7561 ctccggtcta tggacccggt ggaattgttt ccacatctca accgcttgcc actgccgcgg
     7621 gactcgaaat ccttgcgaag ggaggcaacg cggcggatgc cgccgtcgct gctggcgcgg
     7681 cactcaacgt aaccgagcca acctccactg gaatcggcgg ggatatgttc gcgctctatt
     7741 tttctgcgga tacaaaacga gtcaccgctt taaatggatc gggacgcgct cccgccgcgc
     7801 tgacacttga ccggctaaag agtgaaggat tctcgtctga gctgcctcca taccacgcgc
     7861 acactgtgac tgtccccggt gcctgcgcgg gttggtttga cctgatcgaa aggcacggtt
     7921 cactttcgat gggagagatc ctcgcgccgg ctattcgact ggcgagcgaa ggattcccgg
     7981 tcgcgccact gacttccaag tcctggtctt atgcggtgca gcgacaactc aaaaccgcac
     8041 ccaatggaaa tgagttaacc attgatggac gtgagcctcg tccgggagag atttttcgca
     8101 acccaggatt agctcgcaca tttgaaacga tcgcccgtgg aggcaagtca gcctattacc
     8161 agggtgacat cgcggaagcc atcgtcagcg tcatgaaaga agcgggcggg accatgtctg
     8221 ctgaggatct tgcctcgcac gtctccacct gggaggagcc gatctcagtc acgtatcgag
     8281 gattgaacgt ctacgaatgc ccacccaacg gacagggcat caccgctttg atcgcgttga
     8341 acattctcga aggattcgat ttggcgtcac ttgagcaact ctccaccgag cgaatgcatt
     8401 taatgatcga agccatgcgg ctcgcctttg cggatgcaag ttggtatgtg gctgatcccg
     8461 cgctcgcgaa tattccagtg caggaattat tatcgaaaga atatgcagaa gaacgccgca
     8521 acttaatcaa caaaacgcgc gccacgattg accaaaaaca tggcacgcct gttgcatctt
     8581 cggatacagt gtatctaagt gtggtagata agtttggaaa tgcctgttcg ttcatcaaca
     8641 gtaactatat gggcttcggc acaggcatcg tcccgaaagg ctggggcttc accctccaaa
     8701 atcgcgggca taacttcagc cttgacccaa accaccccaa cgccctcgcc ccgcgcaaac
     8761 gaccctatca caccatcatt cccgcaatgg ttactcgcct tccctctccc cgcgggagag
     8821 ggatgtccga aggacagggt gagggggaag aaactttatt cgcctcctac ggcgtcatgg
     8881 gcggattcat gcagccgcag ggacatgtcc aagtcctctc cgcgctcgtg gacgatggac
     8941 tcgacccgca agccgcgctc gaccgtcccc gcttttgcgt cgacgtggaa gaatcaggtg
     9001 ggcgagttgc aattgaagag ggtatgcctg acgaaacgta ttcagggctg gaaagaatgg
     9061 gacatcccgt ttacagagtc acaagctata accgtgccct cttcggcagg gggcaagtga
     9121 ttctccgcga ccctgaaaca ggtgtattgt gtgccggaag cgacccccgc gcggatggat
     9181 gcgcgatgac gttgatttga tgccgtcatt gcgaggcgtg ccgaaggcac aacgtggcaa
     9241 tctcctcaca tttggatgtt gcgcgggtta ttatcatcct catcccacaa tgacggattg
     9301 gcttcaatgt aatcggtttt gttctgtaaa tctttttcgt cgcggatgat gtgttcataa
     9361 taattgcgtt gccagatacc ggtggcgttg tgttcgcgtc cgatgcgacg tgtgacggcc
     9421 gccttgtatg ttcttataat tgttggaatt gaaccggcca ccggtttttg aaattgttcc
     9481 atcgtagggg cacgatatat cgtgccccta cgaataatga taatcccatg aacatgatta
     9541 ggcataacca catacacacc caattccgcg aacggaaaat gttccggaat cgcgcgccaa
     9601 cattcatctg cgatcttgcc aaaatcgctt aatatcattt tcttttttac aatgtcacca
     9661 aacaaacaat cgcgttgata ggtaacaatc gtcacgtaat atgctccctc ggacgaataa
     9721 tcatatccct tcaggcgaat tgaccttcgg tgatgtttct taggatcgaa ttttgatttc
     9781 ataatttgca tgtgtatggg acaggctcct cctgcccatg ttattgctgc tacactcgaa
     9841 cattaatccc ttttgacgat tgccccaacc tctgtgaccg ttttcaagaa cgcgaatggc
     9901 acgatgaacc attctgtaac gaacgccgcc agaaatatcc cgatgaaaca tgataagaac
     9961 aggatcgcgc ttcgacccat gctttgttca aggctggcat aagcactttc tggtgttacc
    10021 gaatcgaccc taaaaagatc cacgatgaag ctgatgattt gaaaggcagc gatacctgca
    10081 atcgctgatg caacgacgga tatcgagatt tccgcgcgtt ggataccgaa caaaaatacc
    10141 ccaataaata tccctgggac gagggcaaat gcaattgaaa agagcaggaa ggattcgagc
    10201 atactccgcc gattccccgc ctgatatcgc cgaatgacgc tattgttcgt ttttattact
    10261 gttttcgtgg atgaaatctt tccatagccg gaatcttctg ctggcgcgcg cagccttacg
    10321 aaaaccgccg ccaggaggat ggcgatgagc gtctcaagaa aataaatgac cagtgcagtt
    10381 tcggctgaat tcccaccaaa aagaaaggct cctatgggaa tgacatttcg tcccagagca
    10441 gagcctatga agattgcctt tttcattcga tgctttccac ctcccaataa atatataacc
    10501 tttttctagt ttgcgatcct aataccgcgc atccgccatc actaccctcc actcaagtag
    10561 agtaatgcat aacttgccag ccagtgttcc accatgtaat ctgatccggt cacagcgccc
    10621 atgcttgccc gggcatgcga ttcgatgccc gcctcaagat aggatctgcg cggatcgtct
    10681 gcgggcagga tggcgtgcaa atgtttccac acaaatgccc gatacagatt caaaccatga
    10741 agatgcgcca gttgaccatc cgcggcatcg gatacgaagg ccggttcaaa gagagatttt
    10801 ggatgtccgg aggggagccc cggcaaaaac gcatccagcc acgccagaaa tgcctcccgt
    10861 tccagaagaa cgctcatcaa ttccacttcc gtcagggcgg gcgagagaaa atcgcttccg
    10921 ctgggttcat aactggcggg atagtcccga tcctcaccat accattggat cgccgttcta
    10981 gtgatggctg tcaacaggcg tgtatctccc tgctttgcca gaacccgagc ccacggcact
    11041 gaacgcgcca gaccaaacgc gctgttactg tgcatcccgg tccgaatcgg ataggttgcc
    11101 ttcgggagcc atgccaacat caactcagtg atcacgtccg caagcggacg aacagccgcg
    11161 agccattgag cagcctcttt agttttccaa agagtcagtt catacgcgag ggtcaacgcc
    11221 cagccccagc catacgggcg ctcgaaacgc ggtcgggcat gcagatagct gacttcctgg
    11281 gccaatgcct cttttgtcag atgtgcttca agtaccgtga tcgccttcgc attgtcaaac
    11341 gcatccggtg ccaggcgcag gagtcgaatc aacgaccaat gcatgtgaac gctcgaatgc
    11401 cagtcgaaac aaccatagaa ggctgggtga gcttcgcgcg gccgcggacg atcctccgga
    11461 cccgtcatca tgtgttgcat ttgattcgga tattcctgct ggatggcagc gatgatcgtc
    11521 tctgcgtacg tcgacgccac ctgctcaagc atggactttg cgttttgaag agttgtaatt
    11581 tctataaaat tgtcatcctg agcgaagcga aggatctcca gcccaaggta agagaccctt
    11641 cggtcgcgaa ga
//
LOCUS       DASTJP010000679         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1198506, whole genome shotgun sequence.
ACCESSION   DASTJP010000679 DASTJP010000000
VERSION     DASTJP010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198506"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(793..865)
                     /locus_tag="VFQ23_19860"
     tRNA            complement(793..865)
                     /locus_tag="VFQ23_19860"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Glu,seq:ttc)
     gene            complement(926..1468)
                     /locus_tag="VFQ23_19865"
     CDS             complement(926..1468)
                     /locus_tag="VFQ23_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908917.1"
                     /translation="MDTSPRTYRFLPADIYTSGYRVVGKVMISTNGIMGVLNDINKSH
                     LELHDARMARIHMPSKLVDHFEVVRLVKSQIFAICAARREDLGPAGIQRGGYSNISAL
                     PIRITTQVYEIEGKLEVAGRFDFAALIADKTRNFIPVYEAVLTAILIPNLKAESPGVL
                     INREKVDILALIGQRAKEEK"
     gene            complement(1498..1803)
                     /locus_tag="VFQ23_19870"
     CDS             complement(1498..1803)
                     /locus_tag="VFQ23_19870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HET9908918.1"
                     /translation="MNYIQTIPTPSANSFPCRECSAGMTHLRHITYFTWLGEELIMVP
                     NFPAWICDMCGRREYDERAISWLTMLLNPNAGKPTRHPKRTPRPGSAKRNLPRLPLD"
     gene            complement(1800..2408)
                     /gene="lepB"
                     /locus_tag="VFQ23_19875"
     CDS             complement(1800..2408)
                     /gene="lepB"
                     /locus_tag="VFQ23_19875"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9908919.1"
                     /translation="METIQPEAQYQPEPTVEQPANWKRFVLDILETLILAVVLYFGIN
                     AVSARVRVDGFSMRPTLQDGEYILVNKLAYKTGEPRRGDIVVFIFPVNPQEDLIKRVI
                     GLPGESVSVHNGIVSVSGVPLTEPYIASPPAYDGDWVVPEGQLFVLGDNRNDSRDSHQ
                     WGLLPIENVIGRAVLIYWPPEEWQVINQDAQSVAGQKSLSLK"
     gene            complement(2511..>2853)
                     /locus_tag="VFQ23_19880"
     CDS             complement(2511..>2853)
                     /locus_tag="VFQ23_19880"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HET9908920.1"
                     /translation="NVEAVHYRFPIGDFGLPTSERMNNILNKIEERLQAGHRIYLHCW
                     GGIGRTGTTVGCYLVRQGKNADQSLDQLSAWWRGVPKSQFHPQSPETREQVNFIRTWA
                     AQDEKLRESHE"
BASE COUNT          706 a          738 c          710 g          699 t
ORIGIN      
        1 agattcatca tcaaacgttc catccggtcg taacgggatg ttcaaaagca gattgccatt
       61 cttgctgaca atatccaccc gcatatgcac gatgtccgca gttgttttgt gtaccgcctg
      121 ccggttgtca aatcacccgc cgatacaagt gtccgtttgc cggggccggg aatggaccgc
      181 agaaaccact ccatgttcca catctaaaac acctatgccc tcgacggacg ctgtgaatga
      241 cctggataag cttgtcccga tgtgtccata agtctgcact gatccgtgta cggaaaggtg
      301 accgcctttc tatgcgcgga tatattactt agaatcggtc aacaattttc aatggatgcc
      361 tgaagcaaca cgtcctttca ctttattact ggcagtcccg acgggattcg cacccgcgaa
      421 atataatata aggaaagcct cggtaaaagt gattgaaagt gcacatctgg tgttttgccg
      481 taaaagcaac gaatcgcaag ataatcagtc cccaaaaaac tccaatatag gttgcatact
      541 tttaagtatg atactctcaa gtataaccag agcactatcg atactgattg gccgtaagtt
      601 aatcttgaac ggaaaagttt tgcttgacta aagggcattt ctagtagtaa agagattcga
      661 accactcaac actaattttc ataaattctg gctctacaga acagccaaat cattcaactg
      721 tcccctcgaa tcaaacctta acatagacaa aaaccgctca acttcatctg ttggcggttt
      781 ccggcaaact gatgccccca cggggattcg aaccccggtc gtagccttga aagggctgcg
      841 tcctagtcca ctagacgatg ggggcaaact gttaactgag cgggcggatt ttatcatccc
      901 cttatgtaac ggtcaagcgt ttcgtttatt tttcctcttt ggcgcgctga ccgatgagcg
      961 ccaggatatc caccttttcg cggttgataa ggacacccgg gctttccgcc ttcagattgg
     1021 ggatcaggat cgcggtcaga acagcctcat acacgggaat gaagttgcgg gtcttatccg
     1081 cgatgagggc ggcaaaatca aaacgtcccg ctacttcaag ctttccctcg atttcataga
     1141 cctgtgtcgt aatccgtatc ggcagggcag aaatattgct atagccgcct cgttgaatcc
     1201 cggcgggacc caggtcttca cgccgcgccg cacaaatggc aaagatctgt gacttgacca
     1261 gtctgacaac ctcaaagtga tcgaccagct tggagggcat gtggatgcgc gccatacggg
     1321 catcatgcag ctccagatga gacttgttga tgtcgttcaa tacccccatg atgccattgg
     1381 tggaaatcat cacctttccc accacgcgat accccgacgt gtaaatgtcg gcggggagaa
     1441 atctataggt tctgggtgaa gtgtccatgg tgtgtgcctc gaagcccggt gtgttcgtta
     1501 atcaagaggc aggcgaggca gattgcgttt cgcactgcca ggtcgcgggg tccgtttggg
     1561 gtggcgcgtg ggcttgcctg cgttcgggtt cagcagcatg gtcagccagg agatggcacg
     1621 ttcgtcatac tcccgtctac cgcacatatc acagatccag gcaggaaagt tgggaaccat
     1681 gatcaattcc tctcccagcc aggtgaaata cgtgatgtgt cgcaggtgtg tcatgccagc
     1741 cgagcattca cgacagggaa acgaattggc ggagggcgta ggaatggtct ggatgtagtt
     1801 cattttagac tcaacgattt ttgaccggca acactctgtg catcctggtt gatcacttgc
     1861 cattcttccg ggggccaata aatcagcacg gcccggccga tcacgttttc aatcggcagt
     1921 agaccccatt gatgtgagtc cctcgaatcg ttgcggttgt ctcccaggac aaacaattgt
     1981 ccttccggga caacccagtc cccatcgtat gcaggcggac tggcaatata cggttctgtc
     2041 aacggaacac cactgacaga aacaatgcca ttgtggacgg agaccgattc gcctggcaga
     2101 ccgatcacgc gtttgatcag gtcctcctgc gggttgaccg ggaaaataaa aacgacgatg
     2161 tcaccacgcc ggggctcacc tgttttataa gcaagcttat tgacaagaat atattcaccg
     2221 tcctgcaggg tgggccgcat gctgaagcca tccacacgga cgcgggcaga aacagcatta
     2281 attccaaaat ataaaacaac agccagaatc agtgtttcga ggatatccag gacaaatcgt
     2341 ttccaattcg caggctgttc cacggtcggt tcaggctgat attgagcttc aggttgaata
     2401 gtttccaaat cgttagcgcc tccggcaaaa attatacttc cttttggaag gagtctcttt
     2461 acccttaagg ttgagttaac tttgaacaga agcattcata agagcatgct ttactcatgg
     2521 ctttcccgga gcttttcatc ctgcgcggcc cacgtacgga tgaaattcac ctgctcccgg
     2581 gtctccggtg actgcgggtg aaactggctt ttgggaacgc ctcgccacca tgcagataat
     2641 tgatccaggg actggtcggc gttcttaccc tgtctgacga ggtaacagcc cacggttgta
     2701 cctgtgcggc cgattccgcc ccagcaatgc aggtagattc tatgcccagc ctggagacgt
     2761 tcttcgatct tattgaggat gttattcatc ctttcagagg tgggcaagcc aaaatccccg
     2821 atgggaaagc gatagtggac agcttccacg ttg
//
LOCUS       DASTJP010000680         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_168818, whole genome shotgun sequence.
ACCESSION   DASTJP010000680 DASTJP010000000
VERSION     DASTJP010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168818"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFQ23_19885"
     CDS             complement(<1..728)
                     /locus_tag="VFQ23_19885"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9908921.1"
                     /translation="MTSGKVLIIGGGPAGLEAARCVAELGGEAILIEKRERLGGTPDR
                     ENYAKLTHHWRDASEAMTELVACVTASPNVEVKYQAEVKNLSGEEGSFDVQIEATGVA
                     ESLNVGAVIIATGFQHFDPGRATQYYNYYSFPDVITLTDLEKMLKDHCVVRPSNGEPP
                     KKIAFIQCVGSRDRQIGNEYCSKVCCGIASKQAIEVRQQLPDSKVFIFYIDMRMYGYW
                     ENEIYWPAQEKYKVNYVKGVVSEVL"
     gene            complement(803..2026)
                     /locus_tag="VFQ23_19890"
     CDS             complement(803..2026)
                     /locus_tag="VFQ23_19890"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET9908922.1"
                     /translation="MTDSTFTPVEQILDKKSHPVTRNAETAPTHDMHETLQELEAKGE
                     IVLQRVPEPYIEFKTKYGRMKKIPIEHTWHHKSCGHCGHIPGYASSILWLHRQFNLDY
                     HDPTDQTSCTGWNYYASGASNAVGQAAVMARNFAAAYETGYFPLIHCGTSFGHYKEIR
                     EQLVHHKELRDEVRRILDKMGKPLVIPEEIVHYSEWVYAMRHKFAERQLVDMSAIKAT
                     VHPACHYYKIVAEDAIYDPEIYGGQRTAVVTAVLESLGISVEDYSTWFDCCGFGFRHV
                     LVQRDFTRSFAVLRKIEVMKNEVNPDLTVTHDTGCVTTLDKSQFAAKAHDRKVGIPVL
                     SDAQVAALAMGAHPFRVVQFHWHSTDWRPFLEKLGINWQKYWDEFQGDLDAIRAGTKS
                     GITWQDADQPIALTA"
     gene            complement(2120..>2818)
                     /locus_tag="VFQ23_19895"
     CDS             complement(2120..>2818)
                     /locus_tag="VFQ23_19895"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9908923.1"
                     /translation="TLDNWNPGKAENRHPDVSYVEKERLFLEVKSDIRYTDFLYGCYE
                     CGICVSACPSARFYDFSPRVIAQALAREDVERVYDILTEDIWNCAQCFSCVRCPRQNN
                     PGGLVTILREVSLKNGMQESKDALQGYSRVIYKVMLKGNQVSPDMLQPDFFPDWGPWV
                     KDFSENMLVWRKAIPMDSIRGLTDVAWRIDRKTLLELYTIWFESGNMDIIKELDEGLY
                     DLLADVMQEELEEG"
BASE COUNT          652 a          766 c          682 g          718 t
ORIGIN      
        1 agcacttcgc tcacgactcc cttgacgtaa ttgactttat atttttcctg cgcaggccag
       61 tagatttcat tctcccagta accatacatg cgcatgtcaa tgtagaagat gaagactttg
      121 gagtccggca actgctggcg gacttcgatc gcttgcttac tggcaatgcc acaacagacc
      181 ttggagcaat actcgttgcc gatttgtcga tcgcgtgagc ccacgcactg gatgaacgcg
      241 atcttctttg gtggttcgcc gtttgatggc cgcacgacgc agtgatcttt cagcatcttt
      301 tccaggtcag taagggtaat cacgtctgga aatgaataat agttatagta ttgggttgct
      361 cgaccgggat cgaaatgctg gaacccggta gctattatca cagcaccgac atttagagac
      421 tctgcgaccc ccgtcgcttc gatctgaaca tcgaagctgc cttcctctcc agagaggttc
      481 ttcacttcgg cttgatactt aacttcgaca ttgggacttg ccgtcacaca tgcaacaagt
      541 tccgtcattg cttctgaggc gtcgcgccaa tggtgcgtaa gcttggcgta attctcgcgg
      601 tctggtgtcc cacccagacg ttcgcgcttc tctatcaata tagcctcgcc gcccagctcg
      661 gcaacacagc gtgctgcctc caaccccgcc gggcctcccc cgataatgag cactttgcca
      721 gatgtcatgg ttcctccaga agttttccat aaacgcagcg attcaagttc tctccgctac
      781 cgcgaccgct tggctggcta ccctatgcag ttaacgcgat tggttgatcc gcgtcttgcc
      841 aggtaattcc ggatttcgtg cccgcgcgga tggcgtccaa atcgccctgg aattcatccc
      901 agtatttctg ccagttgatt cctagcttct ctaaaaatgg acgccagtcc gtagagtgcc
      961 agtggaattg caccacacgg aagggatgtg cacccattgc cagcgccgca acctgagcat
     1021 cggatagtac agggatgcct actttgcggt catgcgcttt cgccgcaaat tgactcttat
     1081 cgagcgtggt gacacacccc gtgtcatgcg tcactgtgag gtcagggttc acttcatttt
     1141 tcatgacttc gattttgcga aggacagcaa atgaacgtgt gaaatctcgt tgaacaagga
     1201 catggcgaaa tccaaacccg cagcagtcga accaggtcga atagtcttca acactgatac
     1261 ctagggactc aagaactgcc gtcacgacag ccgtgcgctg ccccccatag atttccgggt
     1321 catagattgc atcttccgcg acgatcttat agtaatgaca agctggatgc actgtagctt
     1381 tgatagcact catatccacg agttgccgct cagcaaactt gtgacgcatg gcgtacaccc
     1441 attcactgta gtggacgatc tcttctggaa ttaccagcgg cttgcccatc ttgtccagga
     1501 tgcgccgtac ttcatcacgt aattccttgt gatgtacaag ctgctcacga atctccttgt
     1561 aatgtccgaa gctcgtgccg cagtgaatga gcggaaagta acctgtttcg tacgcagcag
     1621 caaaattacg tgccataacg gcagcctggc ccacagcatt ggaagctccc gaggcgtagt
     1681 aattccagcc agtacaggag gtctgatccg ttgggtcgtg ataatccaga ttaaactgcc
     1741 gatgaagcca gagaatggat gaggcatatc cagggatatg cccgcagtga ccacacgatt
     1801 tatgatgcca ggtatgctca attggaatct ttttcatccg cccatatttt gttttgaact
     1861 caatgtaagg ctccgggact ctctgcaaaa caatctcacc cttcgcctct aattcctgta
     1921 gagtctcatg catatcatgt gtcggggcgg tttccgcgtt gcgcgtgaca ggatgtgatt
     1981 ttttatcaag gatttgctcg acaggtgtaa aggttgagtc agtcatttct tatctccaaa
     2041 acattgactt cacaggtttg atggacatga caggagtttg caccaataaa cttcctgcag
     2101 gcaactgtca aattcggaat tagccttctt ccagttcctc ctgcatgaca tccgccagca
     2161 agtcatacaa tccttcatcg agttccttga taatgtccat gttgcccgat tcgaaccaaa
     2221 tggtgtagag ctcaaggagc gttttgcggt cgattcgcca ggccacatcc gtgagtcccc
     2281 ggattgaatc cattgggatg gctttgcgcc agacgagcat gttctcacta aagtctttga
     2341 cccacggacc ccaatcgggg aagaaatcgg gctgcagcat atcgggggat acctgattgc
     2401 ctttgagcat taccttgtag atcacacgac tatagccctg caacgcgtcc tttgactcct
     2461 gcattccgtt cttgagagag acctcgcgca aaatagtcac caacccaccc ggattgtttt
     2521 gccgcgggca acgcacgcat gagaaacact gcgcacagtt ccaaatatcc tccgtgagga
     2581 tgtcgtagac gcgttccaca tcctcgcgtg ccagggcttg cgcgatgacg cgcgggctaa
     2641 aatcgtaaaa ccgggccgat ggacaagccg aaacacaaat gccgcattcg tagcatccgt
     2701 acaggaagtc ggtatagcgg atgtcgcttt tgacctcgag gaaaaggcgt tccttttcca
     2761 cgtacgagac gtcggggtga cgattttcag ccttgcctgg attccagttg tcaagtgt
//
LOCUS       DASTJP010000681         7874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1148881, whole genome shotgun sequence.
ACCESSION   DASTJP010000681 DASTJP010000000
VERSION     DASTJP010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148881"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFQ23_19900"
     CDS             <1..783
                     /locus_tag="VFQ23_19900"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908924.1"
                     /translation="QTGTTRIALDVGSDAVYFDNPDLPNTRSEMGLPLNLRGQTVGVL
                     DVQSLRPAAFNESDANTLGILADHIAITIDNANLFTQSQQALNELQALYRQYQAQEWE
                     AFTKRETSIGYHQSAISGKLLDRPVVSNEIQEALKNGEVRETKAENGKSESSITIPVK
                     LRGQVIGVLSIKPPRVNRTWNQDEINLMNAISDRLALALDNARLLQESQRRAAKEQKI
                     GDVTAKIGASINMRSVLQTAVEELGRALPGSEVVIQFDSNGK"
     gene            790..2520
                     /locus_tag="VFQ23_19905"
     CDS             790..2520
                     /locus_tag="VFQ23_19905"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908925.1"
                     /translation="MLMKFLAPPIFENDEEKTRVAGLLNAVLLFLIGTIFFIGPISIF
                     LSPAADRLVFAIQFFSGLPFLGLFYVLMKRGYVQAASVGLIAFITVLLLAVSFLEAEL
                     VSATVMSSFIAIATAALFLGPRSLTALVLVFLAGYFIIAIGRSSGWLQPQIPHATEAM
                     NTYITVAINLILGTTALALGSSSLRRAITQSRSASKELNTSNQNLLLLQAELEDRVAQ
                     RTEELANRSQELEMLNLNIQRRASQFEALAQVTQSIISVRDLQEILPHITTVISDNFG
                     FYHIGIFLIDEVNEYAVLSATNSEGGRKMLERKHRLLVGEEGIVGTVTATGEPRIAMD
                     VGTDAVFFNNPELPHTHSEIALPLKSGERIIGALDVQSTATAAFTEEDIQMLGLLASQ
                     VSLAIENARLFDETRNALTKAETVSRQFTREAWGRLHVEQNLLGYRYNLGGATPLDKP
                     VDLAESSNGQDHDRQSDSAQVIVPIELRGELIGKLSVQSPTGTLSPDQIDIIKAVAER
                     VALSAENARLFEETTRRAERERLVSDITGKIRSVSDPQSMIQTAMDELRKVLGASRVE
                     VVPQNIKGSE"
     gene            2564..3331
                     /locus_tag="VFQ23_19910"
     CDS             2564..3331
                     /locus_tag="VFQ23_19910"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET9908926.1"
                     /translation="MSNEKGGVAKTTSTLSLGAALAELNHRVLLIDLDPQANLSLALG
                     LENGAVEMTSANVLIENTALRSAISKTDVQNLDLVPSNSRIESAEQFLPMRSHYLSTL
                     RNAIQATPLPYDYVLLDCPPALGAITLNALSAADLLIIPTQAEYFSAYALRNMMGSIR
                     RVRQESNANLAYRILVTLLDRRNRTHRNIFEQLQATFGQGVFTTVIEIDTKLRESPIA
                     GIPITQYKPTSRGSQQYRVLAQELIEYAKEEANRQTS"
     gene            3297..>7874
                     /locus_tag="VFQ23_19915"
     CDS             3297..>7874
                     /locus_tag="VFQ23_19915"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9908927.1"
                     /translation="MPKKKPIDKRLKNLFEDVKPEQTSTEAKPVSSKRVDSDTPVQRP
                     VANNTSEFARQPVEMASVVTHKESVMTLAFQAGQNSWATLQVLDDTEQRKWSQDEQLL
                     VKQVADQLSLALENARLFQETQRRAEELTVLNELGNELSTKLDPGGIAEVIYKYTSRL
                     MSTKNFYIAVYDEKNDEKSYPLAFEEGQPLDLKPSKVVGKGFTDHIIRNKKIVFAPEN
                     VLSHMKMLGIDFIPLNEDDTPSQCWLGVPMMIGERVLGVISVQSVDTPNLYDNHDRDI
                     LATIASQAAIAVENARLFQEAQRRAQETAALAEVGREISATLNLEIVLERISSYALEL
                     LQATSAAAYMPFERGEKWQAIAAVGLDAIHIKNDPVKRGEGILGKVVTQNIGTIVNDV
                     FQMPDAITINGTEIIDDFEHLMGVPVLSGNRVTGLLAVWRNGEGREFTQTELDFLTSL
                     SLQTAIAIENARLFEESQKALEKARYRAEAENVVNDIATRFLNIDNPTLINQVITENL
                     GRLAEFYNIDRAYIFLFNDDGRTMDNTHEWRQEGIESYIDDFRNVPRDTLPYMMSFLN
                     RIQPFVVPSVADMPEAAGTDKEEYLREHIQSIMCTPVVLQNKLVGFVGLDSVRRQRNW
                     SEEDIFTLRLFGQITIGALDRAHNQEALAKSETDLRALFSSMEDVVLVYDKDGRYTRI
                     APTNPALLFRPPEDMIGKLIQDVLPENLHEPFMTTISRVLAGEEDVNIEYPLTLGGRQ
                     FWFDANVTKLTDDQVFWVARDITERKLSEEALAASQQMLRQVLDTIPSRVFWKDKNLN
                     FIGSNQAFALDAGMQSPEDLVGKNDFDMTWAEQAILYQNDDKSVMESQRPKLNFEEPH
                     TESDGSVTWVRTSKVPLLNTRGQVDGILGIYDDVTAEKAAEEAIRRQNQYLAVSAEIG
                     RLVTSTLDLNTIFSRTVSLISQRFGFYHSSIFIVEETGFNTVLREATGEAGEKMKAQK
                     HSLVIGSGSIVGKAAESGQPIVVNDVEHDPLHRPNPFLPDTQSEATIPLRIGTRVVGV
                     IDIQSTQKNAFSQDDISVLQSLGDQVAVAIDNARSYELSQQLIKDLREIDQLKSQFLA
                     NMSHELRTPLNSIIGFSRVILKGIDGPVTEMQQQDLTAIYNSGQHLLGLINDVLDLAR
                     IEAGKMELNFEEVHLAEMTTSVMSTAKGLVKEKPIQLIQRVPADLPTVRGDTMRVRQV
                     LLNLISNASKFTDEGSITVESTIQKSPTGKLEALVNVIDTGPGISEEDQDKLFKAFSQ
                     VDGSATRKSGGSGLGLSICANLVQLQGGRIGIHSEMGRGSTFWFTLPLYHQPKESIPE
                     GKKVVLAIDDDPQVISLYERYLTPQGYHVEPLTDPSKARERILEVKPFAVTLDIMMPS
                     LDGWTVLTALKSDPATRNYPVVICSITEKADKGFSLGAADYLVKPILEEDLVQALDRL
                     NKNGEIREVLVIDDDPNDLRLIGKILNEQGRYKPILIEGGRRGWEAINEKTPHAIILD
                     IFMPEMDGFTILENLREHPTLRDVPVLVVSGG"
BASE COUNT         2170 a         1879 c         1924 g         1901 t
ORIGIN      
        1 cagactggga cgacacgcat cgcgctggac gttgggtcag atgctgttta ctttgacaac
       61 ccagatctcc caaatacacg ttctgaaatg gggttgccgc tcaatttgcg cggtcaaacg
      121 gtcggagttc tagacgtgca aagtcttcgg cccgctgcat ttaacgagtc ggatgcaaat
      181 acattaggta tcctcgccga ccatattgca attacgattg acaacgcaaa tctcttcact
      241 caatcgcagc aggcattgaa tgaattgcaa gccttatacc gccaatatca ggcccaggag
      301 tgggaagcat tcacaaaaag agagacaagc attggctatc atcagtccgc catcagcgga
      361 aaattgctgg atcggccagt tgtatcaaat gaaatccagg aagcactgaa aaacggcgag
      421 gttcgcgaaa caaaggcaga aaatggaaaa tccgaatcat ccatcacaat tcctgttaaa
      481 ttgcgcggcc aagtcattgg ggtgttaagt atcaaaccac cccgagttaa ccgcacctgg
      541 aatcaggatg agatcaactt gatgaacgcc atctcggatc gacttgccct ggcattggat
      601 aacgcccgtc tgctgcaaga gtcacagcgc cgagcggcaa aagaacagaa aattggcgat
      661 gtaactgcta agatcggtgc atcgatcaac atgcgaagtg tgttgcaaac tgcggttgaa
      721 gagttaggtc gcgcactgcc tgggtcagaa gtcgttattc agtttgattc gaatggaaag
      781 tagaactcta tgttaatgaa atttttagcg ccacccatat tcgaaaacga cgaggagaaa
      841 actcgagttg ccgggctact aaatgcggta ttgctattct taattggaac aatatttttt
      901 attggaccaa tcagcatttt cctctccccc gcggctgacc gtcttgtctt tgctattcaa
      961 ttcttctcgg gcttgccatt tctgggactc ttttatgttc taatgaaacg cggctatgtt
     1021 caagccgcga gtgttggtct gattgctttc attacagttt tactgcttgc tgtgagcttt
     1081 ctggaagctg aactggtaag tgcaactgta atgagctcat ttattgccat tgctacagct
     1141 gcattatttc tcggacctcg atcgttaacc gctttggtac ttgtgttcct ggctggctat
     1201 ttcatcatcg cgattggtcg atcctccggt tggctgcaac ctcaaatacc gcatgccacc
     1261 gaagcgatga atacctacat tactgttgct attaatttga ttctggggac aaccgccctg
     1321 gctttaggct ccagcagttt acgtcgtgca atcacccaat cgcgatctgc ttcaaaagaa
     1381 ttgaatacta gtaatcaaaa cttgctctta cttcaagctg agttagaaga ccgcgtggca
     1441 caacgaacgg aagaattagc aaaccgctct caagagttgg aaatgctgaa tcttaatatc
     1501 cagcgtagag caagccagtt cgaggcgctg gctcaagtga ctcaatccat catttctgtt
     1561 cgtgaccttc aggaaattct gccacatatt acgacggtga tcagtgataa tttcggcttt
     1621 taccacattg gtatattcct gattgacgaa gtcaatgaat atgccgtact gagcgccacg
     1681 aatagcgaag gtggccggaa aatgctcgag cgaaagcatc gcctgctcgt cggtgaagag
     1741 ggcattgtgg gtaccgtcac agcgaccggc gaacctcgca ttgccatgga tgtgggaacc
     1801 gatgcggttt tcttcaataa tcccgaactc ccacacacac actctgaaat cgcacttcca
     1861 ttgaaaagtg gtgagcgtat catcggcgca ctggatgtgc aaagtacggc gacagccgca
     1921 ttcacggaag aagatattca gatgttagga ctgctggcaa gccaggttag tcttgccatc
     1981 gaaaatgctc ggctttttga cgaaactcgg aatgccctga ccaaagcaga aacggtcagt
     2041 cgtcagttta ctcgtgaagc ctgggggcgc ctgcacgttg aacagaattt gctcgggtat
     2101 cgatacaatc ttggcggagc aacaccgctc gataaacctg ttgacttagc cgaatcgagt
     2161 aatggacaag accatgatcg tcaatcagat tctgcccagg ttatcgtacc catcgagcta
     2221 cgcggcgaac tgattgggaa attatctgtt caatctccca caggaacttt gagcccggat
     2281 caaatcgaca tcatcaaggc ggtcgcggaa cgagttgcat tgtccgccga aaatgcacgg
     2341 ctctttgaag aaactacgcg ccgtgcagaa cgtgaacggc tcgtgtcaga cattaccgga
     2401 aaaattcgta gtgtgtcgga tccgcaatct atgattcaaa ctgccatgga tgaacttcgc
     2461 aaggtgctag gtgcaagtcg cgttgaagtt gttccgcaga atattaaagg ttcagaataa
     2521 accgtgtttt tgagagtgag gaaatatggc ttttaccatt gcaatgtcaa atgaaaaagg
     2581 cggggtcgca aaaacgactt cgacattatc tctgggcgcg gcacttgccg agctaaacca
     2641 ccgcgtcctt ttaatcgacc ttgatccgca ggcaaatttg agcctggccc ttgggcttga
     2701 aaatggtgcg gttgaaatga catccgccaa tgtgttgatt gaaaacacgg cactgagatc
     2761 agccattagt aaaactgatg tccagaattt ggatctcgta ccatcgaaca gccggataga
     2821 aagcgcagag caatttttac ccatgcgcag tcattatctt tccacgctcc gaaacgccat
     2881 tcaagccact ccacttccct atgattatgt tcttctcgat tgtccgcctg cactgggagc
     2941 aatcactcta aatgccttat cagcagcaga tttgctcatt attcccactc aggcagaata
     3001 tttttccgcc tatgcgttga gaaatatgat ggggtcaatt cgtcgggtgc ggcaggagag
     3061 taatgctaac cttgcctacc gtatccttgt cacgttgctg gatcgtcgaa atcgcacaca
     3121 ccgaaacatt ttcgaacaac tacaggcaac tttcggacaa ggggtgttca ctacagtcat
     3181 agaaatcgac acaaaacttc gtgaaagccc aattgcaggt atacctatta cacaatataa
     3241 gccaaccagt cgcggatcac aacaatacag ggtgcttgct caggagctaa ttgaatatgc
     3301 caaagaagaa gccaatcgac aaacgtctta agaatttatt tgaagacgta aagcctgagc
     3361 agacttccac agaagcaaag cctgtttcat ccaagcgcgt cgactcggat actccagtac
     3421 agcggccggt agcgaacaac acaagcgagt tcgcaaggca acccgtggag atggccagcg
     3481 ttgttactca taaagagtct gtaatgacgc tcgcatttca ggccggacaa aactcctggg
     3541 caacactaca agtattagat gataccgaac agcgcaagtg gagtcaggat gaacaactgc
     3601 ttgtaaaaca agtggcagat caactttccc tggcacttga aaacgcacgt ctatttcagg
     3661 aaactcaacg gcgtgcggaa gaattaaccg ttctcaacga actgggcaac gaactttcga
     3721 ccaagctgga tccgggcggg atcgcggaag taatttataa gtacacttcg cgtctgatga
     3781 gcacgaaaaa tttctacatt gcagtgtatg atgaaaagaa cgatgaaaaa tcctacccac
     3841 tcgcatttga agagggtcaa ccactggatc taaaacccag caaagttgtt ggtaaaggct
     3901 ttaccgatca tattattaga aacaagaaga tcgtctttgc gccagaaaat gtcttaagcc
     3961 acatgaaaat gctggggatt gatttcatcc cgcttaatga agatgacaca ccgtcgcaat
     4021 gctggctcgg tgttcccatg atgatcggag agcgcgttct cggtgtaatt tcagtccaaa
     4081 gcgttgacac accgaatctc tatgataacc atgatcgtga tattctcgca accattgcaa
     4141 gtcaggccgc gatcgctgtt gaaaacgcac ggttatttca agaagcacag cggcgtgcac
     4201 aggaaacagc cgcgctggca gaggtggggc gcgaaatttc tgctacgctc aatcttgaaa
     4261 ttgtactgga acgaatctca tcgtatgcgc ttgagttatt gcaggccact tcggcagcag
     4321 cgtacatgcc ctttgaacgc ggggaaaagt ggcaggcaat tgcagcagtt gggttggatg
     4381 caatccatat aaaaaacgat cctgtgaaac gaggggaagg catactggga aaagtagtaa
     4441 cccaaaatat tgggacaatt gtgaacgacg ttttccaaat gcctgacgcc atcacaatca
     4501 acggtacgga aatcatagac gactttgaac atctgatggg cgtgcccgtc ttatcaggca
     4561 accgagtgac tggtttgctg gctgtctggc gaaacggcga agggcgcgag ttcacgcaaa
     4621 ccgaattgga ctttttgacc agtctttcac tgcaaacggc gattgccatt gaaaatgcac
     4681 gtctctttga ggaatctcaa aaggctctgg aaaaagcgcg ctaccgtgca gaagcagaaa
     4741 atgtagtgaa cgatatcgct acaaggtttc tcaatattga taacccaacg ctgattaatc
     4801 aggtcatcac cgagaacctc ggacggcttg ctgaattcta taacattgac cgggcctata
     4861 tctttctgtt caacgatgac ggccgtacga tggacaatac acatgaatgg cggcaggaag
     4921 gcatcgagtc gtatattgac gacttccgca atgtgccgcg agacacattg ccttacatga
     4981 tgagttttct caaccgaatc caacctttcg tggtccccag cgtagccgat atgcctgagg
     5041 cagcaggaac cgataaagaa gaatatttga gagaacatat tcaatccatc atgtgcacac
     5101 cggtggttct gcaaaacaaa ttggttggat ttgtcggatt ggattccgtt cgtcggcagc
     5161 gaaactggag tgaagaggat attttcactt tgcgactttt cggtcaaatc acaattggtg
     5221 cactggaccg tgcccacaac caggaagcgc tggctaaatc tgaaactgac ctgcgagcac
     5281 tcttttcttc catggaagat gttgttttgg tatacgacaa agatggccgt tatacccgca
     5341 ttgccccaac caaccctgcg ttgctgttca ggccgcctga agacatgatc ggaaagttga
     5401 tccaggatgt tcttccggaa aatctgcacg aaccgttcat gaccacaatt agcagagttc
     5461 tcgccggcga ggaagatgtg aacatcgagt atccgcttac attgggcggc cggcagtttt
     5521 ggtttgatgc caatgtgaca aagctcacgg atgatcaagt attctgggtc gcacgtgata
     5581 ttactgaacg caagctatca gaggaagcgc tcgcggcttc tcaacaaatg ttgcgccagg
     5641 tgctggatac gatcccatcg cgtgtgtttt ggaaggataa gaatcttaac ttcattggta
     5701 gcaatcaggc attcgcgctt gacgcgggaa tgcaatcacc cgaggatctg gttggcaaaa
     5761 acgatttcga tatgacatgg gcggagcaag caatcctcta tcaaaacgac gacaagtcag
     5821 taatggaaag ccagcgccca aagctgaatt ttgaggaacc tcacactgaa agtgatggaa
     5881 gcgtcacatg ggttcgcaca agtaaagtgc cattgctcaa tacgagagga caggtggatg
     5941 gcatcctggg gatctatgac gatgtcactg ctgaaaaagc agcggaagaa gctattcggc
     6001 gccagaacca gtacctggcg gtatctgccg aaatcggtcg ccttgtcaca tctacgctgg
     6061 acttgaatac catcttctca cggactgtga gtcttattag tcaacgcttc gggttttatc
     6121 attcctccat ttttatcgtt gaagagacag gcttcaatac agtgcttcgt gaagccacag
     6181 gcgaagctgg cgaaaagatg aaagcccaaa aacattctct ggtgatcggt tctggttcta
     6241 ttgttggtaa ggcagcggaa agcggtcaac cgatcgtcgt aaacgatgtg gagcatgatc
     6301 cccttcatcg tccgaacccc ttcctgcccg atacgcaatc cgaagccaca atccccttac
     6361 gaattggaac acgcgtcgtt ggtgtaattg acattcaatc cacacaaaag aacgctttct
     6421 cacaggatga tatttcggtg ctgcaatcgc tcggtgacca ggtcgctgtt gcaatcgaca
     6481 acgcccgttc ctatgaactc tcccaacaac tgattaaaga cttgcgtgaa atagaccagt
     6541 tgaagagtca gttccttgca aacatgagtc acgagctacg gacccccttg aactcgatca
     6601 tcggtttttc acgcgtcatt ctcaaaggca tcgatggacc tgtaacggag atgcaacagc
     6661 aggatctgac ggctatctac aattccgggc agcacctctt ggggttgatc aatgacgttt
     6721 tggatctggc acgcattgaa gccggcaaga tggaacttaa ctttgaggaa gttcacctcg
     6781 ctgaaatgac gacgagtgtc atgtcgacgg ccaagggtct tgtcaaagaa aaaccgatcc
     6841 agttgattca gcgtgtcccc gcagatctgc cgaccgtgcg cggtgacacc atgcgcgttc
     6901 gtcaggtatt acttaacctg atctccaatg cttcgaaatt taccgatgaa ggctctatca
     6961 ctgttgaatc caccatacaa aaaagtccaa caggaaaact ggaagcattg gtcaatgtga
     7021 ttgatactgg gcccggtatt tctgaagagg accaggataa attgttcaaa gccttttcac
     7081 aggtcgacgg atctgcgact cgcaagagtg gtggttcagg gctgggactc tcaatttgtg
     7141 caaacctcgt acagttacaa ggcggcagga tcggtatcca tagcgagatg ggaagggggt
     7201 caaccttctg gttcacacta ccgctttatc accagcccaa ggaaagtatt ccggagggta
     7261 aaaaagtcgt cctcgcgatc gatgacgacc cgcaggtcat tagtttgtat gagcgctacc
     7321 ttacaccaca gggttatcat gttgaacctc ttaccgatcc gtcgaaggcg agagaacgga
     7381 ttttggaagt caagcctttc gcagtcactc ttgacatcat gatgcccagc ctggatggat
     7441 ggaccgtgtt gactgccctc aaatctgacc ccgccacgcg aaattatccg gttgtgatct
     7501 gctcaatcac tgaaaaagca gacaaaggct ttagtcttgg cgccgcagat tatttggtca
     7561 agccgatcct cgaagaagat cttgtgcagg cactggatcg tctgaacaag aacggagaaa
     7621 tccgcgaagt attggtcatt gatgatgatc caaacgacct gcgcctaatt ggaaaaatac
     7681 ttaacgaaca aggtcggtat aagccgatcc tcatcgaagg tggacgacga gggtgggaag
     7741 ccataaacga gaaaaccccg cacgcgatca tcctggatat ttttatgcct gaaatggatg
     7801 gctttacaat tctggagaat ctgcgtgagc accccacact gagagatgtt ccggtgctag
     7861 tcgtcagtgg cggc
//
LOCUS       DASTJP010000682         8216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1114110, whole genome shotgun sequence.
ACCESSION   DASTJP010000682 DASTJP010000000
VERSION     DASTJP010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114110"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFQ23_19920"
     CDS             <1..219
                     /locus_tag="VFQ23_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908928.1"
                     /translation="RASNDLQCEASLVFPPMELVGFDSVWKQVGYERRSPETLGVQAW
                     TDAAMESMPRGSALFFKQLRTDRVLRPI"
     gene            358..1227
                     /gene="cdaA"
                     /locus_tag="VFQ23_19925"
     CDS             358..1227
                     /gene="cdaA"
                     /locus_tag="VFQ23_19925"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET9908929.1"
                     /translation="MANFLDNIFFIFQRLSWLSLLDILLVTLIFFVLLYSLRDTQAMV
                     LVRGVIFLVVALVLLTTLVELPAFSWLIQNALPALLLAIPVIFAPEIRRALERLGRAS
                     TFSPTTSQTSYEDMQKVISALVGASARLSARQHGALVVIQRMDNLQQYIETGVRMGAE
                     ATPELLLQIFYPDTPLHDGAVIVADGRIIAAACVLPLSASGILNRSPERQMGLRHRAA
                     LGISEVSDAISIVISEQSGSISISHAGRMIRRIDPDRLENILVAFFRPDATTPQRIWL
                     QRFFPYLFQRKDE"
     gene            1260..2498
                     /locus_tag="VFQ23_19930"
     CDS             1260..2498
                     /locus_tag="VFQ23_19930"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HET9908930.1"
                     /translation="MQWLATNVRTFLLAFVLGISVWLSAVTGADPDDVLVYPKPVKLE
                     VIGQDPSLVLTSDIPSTIQVTLRAPRSVWEQLTAQENSVTSILDISGLSAGEHTQTVQ
                     LRIAGRPVQVVLMTPETITFTLEALSNQSLPVEIDLTGQPAVGFQAGDPLIEPNQIAL
                     SGPESIVRKAARARVLVNLDSKRENIDGDFDIQVMDDENRILNGISINPESAHIEIPL
                     SPQSGYRDLAVKVVVNGQVAAGYRLENISVFPQVITVFSLDAELINELPAVVDTQPLD
                     LQDAKDNISTRLALTLPETITIVGTKTVEVQVDITPIETSLTLMDQAINIIGLPNGFD
                     AQISPETVNVIISGPLPVLDALTAQDIKVSVDVTGLDLGTHQIEPKVEVFVEDVLEGS
                     ILPSTVEVVLSIRPTATPIP"
     gene            2541..3893
                     /gene="der"
                     /locus_tag="VFQ23_19935"
                     /pseudo
     CDS             2541..3893
                     /gene="der"
                     /locus_tag="VFQ23_19935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
     gene            4014..4373
                     /locus_tag="VFQ23_19940"
     CDS             4014..4373
                     /locus_tag="VFQ23_19940"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9908931.1"
                     /translation="MKDFRQLKVWEKAHQLALAIYKETKKFPKEELYGLTSQIRRASM
                     SIPTNIAEGCGRNTDAEFARFLQIAMGSASETEYQLLLSHDLGFLNKHQYDNLNLDVT
                     EIKRMLVSLLKTLRADR"
     gene            4373..6001
                     /locus_tag="VFQ23_19945"
     CDS             4373..6001
                     /locus_tag="VFQ23_19945"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET9908932.1"
                     /translation="MPDSFDYVIIGSGFGGSVSAMRLTEKGYSVLVLEKGKRYEDKDF
                     AKTNWHYWKYLWAPALHAYGILQVSILKGVMVLHGAGVGGGSLGYANVLEIPSNETFA
                     TPAWNRGMNWGKVLRPHYETAKKMLGVARNPRLWTADDILKQIAEKSGMGYTFRATDV
                     GAYFGEAGVTVPDPFFGGEGPERAGCTHCGGCMVGCRYNAKNTLPKNYLYFAEKNGAK
                     VQAESEVVDVRPWVERAHYEVTYQSSTAAIKRKHKVYAKNVIFSAGVMGTMRLLLDLR
                     DVKQSLPKLSGRLGQMVRTNSEALLGSVARRSDVNYSEGVSISSIYNYDEITRVEPVR
                     YPDGSSLMRFLAAPMIDRDASIPVRLLRFFIWALTHPMDFLKAMVLPGWAHNATILLV
                     MQHADNRMRFRMGRSLYTLFRRGMVAEKEPGYEIHAHVKGSHELTRNFAKMTNGVALG
                     SLGENLLGLPTTAHILGGAPIGRDSSEGVVNERFEVHNYPGLYIIDGSIMPANPGVNP
                     SLTITALAEYAMSKVEPKLAEVQIKRKNVQLESI"
     gene            6004..6606
                     /locus_tag="VFQ23_19950"
     CDS             6004..6606
                     /locus_tag="VFQ23_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908933.1"
                     /translation="MAGSTLQELWGFDDTDLASNRNGKLSEKQSTFLASEHKSQSRVF
                     LGVAGFVVAIFCCLPALLFGSSGFLPLLVSGNLSDLTEPLSAFATGGVALSFVALFAL
                     PIVAVVVIYLMRANKKADTSVNRAEGTAMYTWGTKRVRTPTRLRKYEDVKVLHLNLGD
                     KKFEVKEALQGLIREGEPWMIYYTSYPFKFLSAEVVSKTE"
     gene            6610..7641
                     /locus_tag="VFQ23_19955"
     CDS             6610..7641
                     /locus_tag="VFQ23_19955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908934.1"
                     /translation="MDSLNSFTLRPYLAADVQSVVAVINADSQKTLGYPRAVIDGVGN
                     MRMVRYVPLSSDKVVAVNEQGTIVGYAYAADKENGIVSEVGGAVHPEEWGKGIGQLLL
                     DWAEAQARVLSNNAPAGVRSVLQANLFETELEAIKLFTDSGYVKMREWVHMSLDMDQL
                     FPVLSLPDGLKLREMDLDNDWDIVGPAMDEAFADHWGAIQLSAISEESVDTANEEDDE
                     EMDDTPGDDSFSNAPGYCFIILDGDKVAGGILCNAKLVERTDTGRVGSVFVRREYRRR
                     GIGQALMKTAFNTFWQHGIKRIILDTDSESFSESTKFYGGLGMKLYRREFLYEKEIRP
                     GKEVRRLHV"
     gene            7638..>8216
                     /locus_tag="VFQ23_19960"
     CDS             7638..>8216
                     /locus_tag="VFQ23_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908935.1"
                     /translation="MNNFSASRDFATQLDAQDPLAAYRDQFVDNDPDLIYLDGNSLGR
                     MPKVVVERMKKAVEEEWGTDLIRGWNKGWWDAPSRIGEKIATLLGAAEGQVVVGDQTS
                     INLFKLVISALNLQPNKKRIITDAFSFPSDLYILQGIVELLSPSPPSPLPMGEGGYEI
                     IRIGASDDDITPDLAALETAITEDTALVTLSHV"
BASE COUNT         2118 a         1958 c         2110 g         2030 t
ORIGIN      
        1 cgggcttcga atgaccttca atgtgaggca tcactggtct ttccgcccat ggaactcgta
       61 ggctttgact cggtgtggaa gcaggtcggc tatgaacgtc gttcgccaga aacgttggga
      121 gtgcaggcat ggactgatgc ggcaatggag tcgatgccca gaggctctgc tcttttcttc
      181 aaacaacttc gcacggaccg tgtcctcagg ccaatttaga cgtctatgta aggccttgta
      241 tcgacagaaa tcttgcaggg agggaagttg ttttttatcc gtggatagac atcgtcacaa
      301 ttctgatatc taatcccaaa tttagccagc aagactatac tgattaacca ctgaccaatg
      361 gcgaattttc ttgataacat cttttttata ttccagcgcc ttagttggtt aagcctgctc
      421 gatatcctgc tggttacact cattttcttc gtgctcttat attcacttcg cgatacacag
      481 gcaatggtgc tggtaagggg tgtgatattt cttgttgtag cgctggtatt actcacgacg
      541 ctcgttgagc ttccggcgtt ttcctggcta atccaaaacg ctttaccagc cttgttactt
      601 gccattccag tgatctttgc accagagatc cgccgcgcgc tcgaacgatt gggacgtgca
      661 agcacgttct cacccacaac aagccagacg tcctacgaag atatgcagaa agtgatcagt
      721 gccctggtag gagcgtcggc gcgtctctct gcacgtcaac atggtgcgct ggtcgtgatc
      781 caacgcatgg ataaccttca gcagtatatt gaaactggtg taaggatggg agccgaagcg
      841 acacctgaat tgctgctaca aattttctat cctgacactc ccttacatga cggagccgtc
      901 atcgtcgcgg acggccgcat catcgctgca gcttgcgttc tgccattatc agccagtggc
      961 attctgaacc gaagccccga acggcaaatg ggacttcgcc atcgcgcagc gctgggcatt
     1021 tcggaagtga gtgatgctat ttctatcgta atttcagaac aaagcggatc gatatccatt
     1081 tcgcatgcgg gtcgcatgat cagacgcatc gatcccgacc gcctggaaaa tatcctggtc
     1141 gcgttttttc ggcccgatgc tactacacct cagcgcatct ggcttcaacg gttttttcca
     1201 tatttatttc agagaaagga tgagtaggat cgaggtcaac tcgtccataa aaaattttca
     1261 tgcaatggct tgccactaac gttcgaactt ttctgctggc atttgtgctg ggaatttcag
     1321 tatggttatc ggccgtcacc ggcgcggacc ccgacgatgt gctcgtctat ccaaagccgg
     1381 ttaaactcga agtgatcggg caggatccat ctctcgtcct gaccagtgac atcccctcga
     1441 cgattcaagt gactttgcga gcgccgcgtt ctgtgtggga gcagctcacc gcgcaggaaa
     1501 attctgtcac cagcatattg gatatatcag ggctcagcgc gggcgaacat actcaaacgg
     1561 tgcagctacg tattgcgggt cgaccagttc aggtggttct gatgacccct gagacgatta
     1621 cctttaccct ggaggcatta tcaaaccaat ctttgcctgt tgagattgac cttaccggtc
     1681 aaccggcggt tgggtttcaa gcaggcgacc ctctgatcga gccgaatcaa attgcgctct
     1741 cggggcccga atcgattgtt cggaaggcag ccagagcgcg cgtcctggtt aatctcgata
     1801 gtaaacggga aaacattgat ggagattttg atatccaggt tatggatgat gagaacagga
     1861 tattaaatgg catttccatc aacccggagt cggcgcatat tgagattcct cttagcccgc
     1921 aaagcggcta tcgcgacctg gcggtgaaag tcgtcgtcaa tggacaagta gcggcgggct
     1981 atcgccttga aaatatttct gtcttcccac aagtaatcac ggtcttctcg ttggacgcag
     2041 agctgataaa cgaattaccc gctgtagttg acactcagcc actcgatctc caagacgcca
     2101 aggataacat ttcaacccgg ctggcgttaa cacttccaga aacgataacg attgtgggta
     2161 caaagacagt ggaggtacaa gtcgatatca cacccatcga aaccagcctt acacttatgg
     2221 atcaggctat caacataatt gggttgccaa atggatttga tgcacagatt tcacctgaaa
     2281 cggtaaatgt cattatctct ggtcccctgc ccgtactcga tgcgctaact gcgcaggata
     2341 tcaaagtcag tgtagatgtg accggtctgg atctcggcac tcatcaaatt gaacccaaag
     2401 tggaagtgtt cgttgaagat gtattggagg gttcgattct acccagtact gtggaagtcg
     2461 tcttgagcat ccgccccacg gcgactccca tcccataact ttagtctgat tggaaatctt
     2521 acatgtcaaa gcccattgtt gcactcgtcg gcagaccgaa cgttggcaaa agcactttat
     2581 tcaatcgcct ggttggcgaa cgtcttgcca ttgtggatga cacccctggc accacccgtg
     2641 accgactctt cggcgaagtg gagtggaatg gtcgcgtgtt tcacgtcgtt gacaccggcg
     2701 gcatcgaccc aacacatgga ggcaaaactc ctttatctgt gggctccgct gacttcatca
     2761 atgagatccg cgctcaggca aaggtcgcca ttgacgaatc ggacgccgtc ttatttgtca
     2821 ctgatggaga ggcaggtgta acggctcctg atctggaagt ggcagatatc cttcgccggt
     2881 cacagaagaa gctggagaac ggtacgttct ggcctccgat ttttgttgtt gtcaataaag
     2941 cggaatcaaa atcgcggcgg gacgagtctc ctcaattcta cgaactgggg ctgggcgacc
     3001 cgtttgccgt ttccgctgtg catggaacag ggacgggcga tctactcgac gcgctggtcg
     3061 agtcatttcc acaacaggag accgaagagg aagatgacag cgtcaagatt gcgctggtcg
     3121 gcaagccgaa tgccggcaaa tccagcttgc tgaataaact tgttggcgag gaacgcgcga
     3181 ttgttagtcc gattcctggt acaacgcgcg atgcgaccga tacgatcatt gatgtaaatg
     3241 gtttacctgt tactttaatt gacaccgcag gcatccgcaa acgcggcaag atcgaacacg
     3301 gcgtggaaca atacagcgtg ttgaggtcct tcaaagccat cgagcgtgcg gatgtggctc
     3361 tgctgatgat cgacgcgaca accggtatca ccgcccagga cgcgcatatc gcaggcttta
     3421 tcctggacga atggaaatca tgcgttgtca ttgtcaacaa atgggacgca gtcgaaaaag
     3481 attcttactc catggaagaa ttcacaaaaa ccattagacg tgacttgaat tttatggatt
     3541 acgtcccgtt attattcatt tcagcgaaga caggtcaacg cgtggatcaa gtgttgccca
     3601 tggcgttgcg tgtacaggaa gaacgtcttg cacgattaac aacctccaag atcaacgagg
     3661 tgattcacaa cgcccaggac gcccatcccc acccatcgca cgcaggacgg cagttgaaaa
     3721 tgttctatgg cacacaggtg cgaagcgacc cgccaacgtt catgatctac gtgaacgaac
     3781 caaagttgat gcacttcacc tatttgcgct acctcgagaa tcaaatccgc gcggaatacg
     3841 gcttcttagg cacgccgatt cgaattgtga ccaaagggag gagggagaag taattcgtgc
     3901 cggggagtag aagttgattt tccctcttat cagggccaga aatcagatag cggtcagcta
     3961 tcagcattga gctaaaaact gactgctttt cgttgaccgc ttataattac aaaatgaaag
     4021 actttcgtca actcaaggtg tgggaaaagg ctcatcaatt ggctctagcg atctataaag
     4081 aaacgaagaa atttcccaaa gaggaacttt acggattaac ttcacagatc cgccgggcga
     4141 gcatgtccat tccgacaaac atcgccgaag gatgtggacg aaatacagac gctgaattcg
     4201 cacgctttct acaaattgca atgggatcag ccagcgaaac agaatatcaa ctgcttttat
     4261 cacatgacct tgggttcctc aataaacacc aatatgacaa tctgaattta gatgtcacgg
     4321 agatcaagcg catgcttgtc tcactcctaa aaacactgag agctgatcgc tgatgcctga
     4381 cagctttgat tatgtaatca tcggctccgg atttggggga agtgtctcgg caatgcgctt
     4441 gaccgaaaaa gggtattccg tactagttct tgaaaaaggc aaacgctacg aagataaaga
     4501 ctttgcgaaa accaattggc actattggaa atatctttgg gcaccagcct tgcatgccta
     4561 tggaattttg caggtcagta ttctcaaggg cgtaatggtg ctgcatggag caggcgtggg
     4621 tggcggaagt ttggggtatg ccaatgtcct tgaaatacca agtaatgaaa cttttgcaac
     4681 gcctgcatgg aataggggca tgaactgggg taaagtcctg agacctcatt atgagacggc
     4741 aaagaagatg ttgggcgtcg cacgcaatcc cagattgtgg actgcagatg atattctcaa
     4801 gcaaatcgct gaaaaaagcg ggatgggata caccttccgc gcaaccgatg tgggtgctta
     4861 ttttggtgaa gcgggcgtca ccgttccaga tccttttttt ggcggcgaag gaccagagcg
     4921 cgccgggtgc actcactgcg gcgggtgcat ggtgggatgt cggtataacg ccaagaacac
     4981 acttccaaaa aactatctct attttgccga aaagaatggt gcgaaagttc aagccgagtc
     5041 agaagttgta gatgtgcgac cttgggtgga acgcgctcat tacgaagtga cgtatcaatc
     5101 atccacagca gcaatcaaac ggaaacataa agtgtatgca aaaaatgtca tcttcagcgc
     5161 gggtgtgatg ggaacgatga gactcttgct tgatttgcgc gacgtcaaac agtctctacc
     5221 gaagctttcg ggtagactcg gccagatggt gagaaccaat tcggaggctt tgttgggaag
     5281 tgtggcacgg cggtccgatg tcaactactc tgagggcgta tccatttcgt ccatctataa
     5341 ttatgacgaa atcacgcgtg tcgaacccgt tcgctacccg gatggatcct cgctcatgcg
     5401 cttccttgcc gcaccgatga ttgataggga cgcaagcatc ccagtccgtc ttttgaggtt
     5461 tttcatttgg gcgttgactc atccgatgga ttttttaaaa gcgatggttt tgccaggctg
     5521 ggcgcacaat gcgacgatcc tgctcgtcat gcaacacgct gataatcgca tgcgcttccg
     5581 catggggcgc agtttatata cgctctttcg gcgcggcatg gtggctgaga aggaacctgg
     5641 ctacgaaatt cacgcgcatg taaaaggttc acacgagctc acacgtaatt ttgcgaaaat
     5701 gacaaacggg gttgcactcg gttcactcgg cgaaaatctg cttggtctcc caaccacggc
     5761 acacatcctc ggcggtgcac cgatcggaag ggattcctcc gaaggcgttg taaacgaaag
     5821 atttgaagtg cataattacc cgggtcttta tatcattgat ggttcgatca tgcctgccaa
     5881 ccctggagtt aacccatcac ttacaattac agcactggca gaatatgcga tgagcaaagt
     5941 ggaaccgaaa ctggctgagg tgcaaatcaa acgcaaaaac gtacaactgg agagcattta
     6001 gacatggctg gcagcacatt gcaggagctg tggggttttg acgatactga tctcgcgtcc
     6061 aatcgaaatg gaaagctcag cgaaaaacaa agcacttttc ttgcaagtga acataaaagc
     6121 cagagcagag tatttttggg tgtagccgga tttgttgtag caattttttg ttgtttgcca
     6181 gctttgcttt ttggtagtag cggtttccta ccccttctcg tttcgggaaa cttatccgac
     6241 ctgactgagc cgttgtctgc gtttgcaacc ggaggtgttg ctctaagttt cgttgcctta
     6301 tttgctttac ccatcgtcgc ggtagtagtt atttatctca tgcgtgctaa taagaaagct
     6361 gataccagtg taaatcgtgc cgaaggcaca gccatgtaca cctggggtac aaagcgcgta
     6421 cgaacaccca cgcgcttaag aaagtatgag gatgttaaag tgcttcactt aaatctgggt
     6481 gataagaaat tcgaagtaaa ggaagcgctt caaggactga tccgagaagg agaaccatgg
     6541 atgatttact acacatcgta ccctttcaag tttttatccg ctgaagtcgt ttcaaagaca
     6601 gaataatcaa tggattctct taattcattt actcttcgcc cctatttggc agccgatgtg
     6661 cagtctgttg tggctgtgat caacgccgat tcacaaaaaa cactgggata cccgcgcgcc
     6721 gtgatcgatg gtgttggcaa catgcgaatg gtccgctatg tgccactttc gagtgacaaa
     6781 gtggtcgcgg ttaatgagca aggcacgatc gtcggttatg cgtacgcggc agataaggaa
     6841 aatgggattg tctcagaagt gggtggggct gttcatccgg aagaatgggg taaaggaatc
     6901 ggtcagctct tattggattg ggcggaggcg caggctcgcg ttttatcgaa caacgcgccg
     6961 gcaggtgtga gaagcgtttt acaagccaat ctgttcgaaa cagagctcga agccataaaa
     7021 ttatttacag attcaggtta tgtaaaaatg cgcgagtggg tgcatatgtc gctcgatatg
     7081 gatcaacttt ttcctgtgct gtctttacca gacggtttga aactgcgcga aatggatcta
     7141 gataacgatt gggatattgt cggtcccgcg atggatgaag cctttgccga tcactggggc
     7201 gcgatccaac tctctgcgat cagtgaagaa tcagtcgata cagcgaacga agaagatgac
     7261 gaagaaatgg acgatactcc aggcgatgat tcgttttcga acgcgcccgg ttattgcttc
     7321 atcatcctcg atggcgacaa agtggccggt ggaattttat gcaatgccaa actggtcgaa
     7381 cggacagata cgggacgagt gggaagtgtc ttcgtccgac gcgaataccg ccggcgcggc
     7441 attgggcaag ctctcatgaa aacggccttc aacacgttct ggcagcatgg catcaaacga
     7501 attattttgg ataccgactc cgaaagtttt tcagaatcca caaaatttta tggcgggctg
     7561 ggaatgaagc tgtaccgccg cgaatttctg tatgaaaagg aaatccgccc gggcaaagag
     7621 gtgagaagat tacacgtatg aacaattttt ctgcctcccg cgattttgca acgcaactgg
     7681 atgcacaaga tccactggca gcttaccgag atcaattcgt agacaacgac ccagacctta
     7741 tctatctgga tggaaactca ttgggtcgaa tgccaaaggt tgttgtggaa cgcatgaaaa
     7801 aagcggtaga ggaagaatgg ggcacagatc tcatccgcgg ttggaacaaa ggctggtggg
     7861 acgcaccctc acgaattggt gaaaaaattg cgacattgct gggcgcagcg gaaggacagg
     7921 tggttgtagg agaccaaacc tccatcaacc tgtttaaact tgttatctcg gctttgaatt
     7981 tgcaaccgaa taagaagcga atcattaccg atgctttcag cttcccatct gatctatata
     8041 tcctgcaagg aatagtcgaa ttattgtcac cctcaccccc atcccctctc ccgatgggag
     8101 aagggggata tgaaatcatt cgcatcggtg cgagtgacga tgacattact ccagacctcg
     8161 cggctcttga aactgccatt actgaagata ctgccctcgt gacactttcc catgtc
//
LOCUS       DASTJP010000683         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_902990, whole genome shotgun sequence.
ACCESSION   DASTJP010000683 DASTJP010000000
VERSION     DASTJP010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902990"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..971)
                     /locus_tag="VFQ23_19965"
     CDS             complement(153..971)
                     /locus_tag="VFQ23_19965"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9908936.1"
                     /translation="MNSIQLTTFSQSSLQDYVDCARRFQLRYLERLSYPAVESEPALE
                     NEKHQQEGEYFHRLIQQYLIGIPKEQISKLANTPNLQRWWDNFVQDFKNLEDFGSLYP
                     EITLSAPLGSFRLLAKYDLIAVRDGKATIYDWKTYRKRPRNEWLSARMQTRVYRALLA
                     KAGAHLNNGQPFGPEQIEMIYWFADFPNDPAKFIYTSAHYQRDWDTFTKLVEEITSAS
                     SFPLTEDRQKCAYCTYRSYCERGIRAGDGEQAEAEMEAEELFDVNFEQIGEIEF"
     gene            1139..1969
                     /locus_tag="VFQ23_19970"
     CDS             1139..1969
                     /locus_tag="VFQ23_19970"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET9908937.1"
                     /translation="MEWITQPETWIAFITLVVLELVLGVDNVIFISILAGKLPAEQQQ
                     RARTTGIALAVITRILLLFSLSWIIGLTEPLFNVFSLEISGRDLVLLAGGVFLIWKAT
                     REIHDKLEGEEGHASARVRSAFWSVIAQILLLDIVFSLDSVITAVGMVDELVIMIAAV
                     LISALTMIFIAGPLSHFVEQHPTVKMLALSFLLLIGFTLIVEGFDVHIPKGYIYFAMG
                     FSVLVEMLNLRLRRVSEAPVALRGAYTRETKSASSQMVPVAAAAAKIPPKKKSGKKRK
                     "
     gene            complement(2019..>3406)
                     /locus_tag="VFQ23_19975"
     CDS             complement(2019..>3406)
                     /locus_tag="VFQ23_19975"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9908938.1"
                     /translation="FIDIRLSRSSNFTSGQVYAEIISKERRGDFLGGTIQVIPHITNE
                     IKRRVASIGKETGADVVIVEVGGTVGDIESQPFLEALRQLRNEFGRENVYFIHVTWLP
                     YIGATGEIKTKPTQHSVAALRSIGISPNMIIARSDYPVDKELCDKIALFCDVEKEAVV
                     PMITSDVLYEVPLLLEKAGVGDYLVEKLNMKATRKPDMKPWEKLVERVRKPKPTVKVA
                     LVGKYVELQDAYMSVREALKHAALANDVEVEIGWVHSADLEKDKGWEIIQNSDGILVP
                     GGFGSRGIEGKIMAARYAREKNVPYLGLCLGMQTMCIDFARGVLNHEDANSSEFDRGT
                     EYPVIDLMLDQRSITDMGGTMRLGSYPCELQDGSKAAEAYGEGEVDERHRHRFEFNNA
                     FKKDFEKGGMVFSGMSPDGKLVEIAEIKDHPFMVGSQFHPEFLSRPMKPHPLFVGFVK
                     AAKETNGNGQH"
BASE COUNT          798 a          861 c          847 g          900 t
ORIGIN      
        1 cgaaggcgaa gatggtttgc ctgtaatcct gaactcaccg attgataatt tcatccccgg
       61 ccgagaaaga ccggtggcaa atcattttgg gatttatata aagtagatgc gtcattgcga
      121 ggaaacgcca agcagttgcg aagaaatgat agttaaaatt cgatctcacc gatctgctca
      181 aaattgacgt cgaacagttc ctcggcttcc atctcggcct ccgcctgttc tccatccccc
      241 gcacgaatgc cgcgttcgca ataggatctg tacgtgcaat acgcgcactt ttgccggtct
      301 tctgtcagtg ggaaggaaga agcggaagtg atctcctcca ccagtttggt aaatgtgtcc
      361 caatcccgtt ggtagtgcgc ggacgtgtaa atgaatttgg cagggtcatt ggggaagtct
      421 gcaaaccagt aaatcatttc gatttgttcc ggcccaaagg gttgaccgtt attcaaatgc
      481 gcaccggcct tggccaacaa ggcgcgatag acccgggtct gcatgcgcgc ggagagccat
      541 tcattacgcg gtcgtttccg ataggtcttc cagtcataga tcgtggcttt gccgtccctg
      601 acggcaatca aatcatattt cgcaaggaga cgaaaactcc ccaacggagc cgaaagggtg
      661 atttcggggt agagacttcc gaagtcctca agattcttga agtcctgtac gaagttatcc
      721 caccaacgct gtaagtttgg tgtattggcc agtttgctga tctgttcctt aggaattcca
      781 attaaatact gctggatgag ccggtgaaaa tactcccctt cctgttgatg tttctcattc
      841 tccagggccg gctccgactc gactgcggga taggataaac gttccagata ccgcagctgg
      901 aatcgccgcg cgcagtcgac ataatcctgc agggatgatt gagaaaaggt ggtcagttga
      961 atagaattca tgttctatcg ttcaattata gcatcaggta atacctcgtc gcgctgcgcg
     1021 tagcgtcggc gcaggatttg aggtacaaaa aatgtatcgt caagggaagc ggctggcgct
     1081 tagattacac agattcggtt atactttggc gaatcaaaaa ttgcgaggag aaataggtat
     1141 ggaatggatt acacaacccg aaacatggat tgcgtttatc acgctcgtag tacttgaact
     1201 agtgctgggt gtcgacaacg tgatttttat ttcaattctg gcaggcaaac tacccgctga
     1261 gcaacagcaa cgcgctcgca ccacagggat tgcgctggca gtaattacgc gtattttgct
     1321 cttgttctct ctctcatgga tcattggact aacagaacca ctgtttaatg tgttcagttt
     1381 ggaaatctcc ggaagagatc tggtattgct ggctggtggg gttttcctga tctggaaggc
     1441 aacacgtgaa atccacgaca aactcgaagg ggaggagggg cacgcctctg caagagttcg
     1501 cagcgcattc tggagtgtga ttgcacagat cctgctgttg gatattgtct tctcactgga
     1561 ttcggtgatt acagcggtag gcatggttga tgaacttgtc attatgatcg cggcggtgct
     1621 catctctgcc ttgacaatga tttttatcgc tggcccgctc agccattttg tggagcagca
     1681 tcccactgtc aagatgctgg cgcttagttt cctcttattg attgggttca cattgatcgt
     1741 tgaaggtttc gatgtgcata ttcccaaggg atacatctat ttcgctatgg gtttttcagt
     1801 gttggtcgaa atgctcaacc tgcggctacg ccgagtttca gaggcacccg tcgctttgcg
     1861 gggcgcctat acgagagaaa caaaatctgc cagctctcag atggtccccg ttgcagcggc
     1921 tgcagcaaaa attccgccga agaagaaatc aggaaagaaa aggaagtagt tcagatcgaa
     1981 aagacttcca gcctacaact ggaagtcttc ttttttgatc agtgctggcc gttaccgttt
     2041 gtttcttttg cggctttcac aaacccgacg aataacggat ggggcttcat cggtcgtgac
     2101 aaaaactccg gatggaactg gctgccgacc ataaagggat gatctttgat ctcggcaatt
     2161 tcgaccagct ttccatccgg cgacatccct gaaaagacca tgcctccttt ttcaaaatct
     2221 ttcttgaagg cattgttgaa ttcaaaacga tggcggtggc gttcatcaac ttcgccttcg
     2281 ccgtacgctt ccgcagcttt tgaaccgtcc tgcaattcgc atggatagct acccaatctc
     2341 atcgtgccgc ccatatctgt aatcgagcgt tgatcgagca tcaggtcaat gaccgggtat
     2401 tccgtgccac ggtcaaactc tgatgaattt gcatcttcat gatttagaac accacgtgcg
     2461 aagtctatac acatggtttg catccccagg caaagtccga gatacggtac attcttctcc
     2521 cgtgcatagc gcgcggccat gatcttccct tcaatgccgc gtgagccgaa cccgccgggg
     2581 acgaggatgc catctgagtt ttggatgatc tcccagccct tgtctttttc aaggtcagcc
     2641 gaatgcaccc agccgatttc cacttccaca tcattggcga gcgcagcatg ttttagggct
     2701 tcacgcaccg acatataagc gtcctgcaat tcaacgtatt tacctactaa cgccactttg
     2761 acggttggtt tcggcttcct cacgcgctcc accagttttt cccatggttt catatcgggc
     2821 ttgcgtgtgg ccttcatatt gagtttttcc accagataat caccaactcc tgctttttcc
     2881 aacaagagag gtacctcgta taacacatca cttgtgatca tcgggacgac cgcttctttt
     2941 tccacatcac agaacaaggc gatcttgtca caaagctctt tatcgaccgg ataatctgag
     3001 cgggcaatga tcatatttgg cgagataccg atcgagcgca gcgcagcgac agaatgctgt
     3061 gttggcttgg ttttgatctc gcctgtcgca ccgatatatg gaagccaggt cacatggatg
     3121 aagtacacat tttcgcgacc gaattcgttt cggagttgtc gcaaggcttc aaggaacggc
     3181 tgcgactcga tgtcaccgac cgtcccgccg acttccacaa tcaccacatc tgcacccgtc
     3241 tctttcccga tcgaggccac ccgacgcttg atctcgtttg tgatgtgagg gatgacctga
     3301 atcgtccctc ccaggaaatc gccgcggcgc tccttgctga tgatctctgc atagacctgt
     3361 ccgctggtga agttgctgga gcggctgagg cggatgtcga tgaagc
//
LOCUS       DASTJP010000684        14137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_902994, whole genome shotgun sequence.
ACCESSION   DASTJP010000684 DASTJP010000000
VERSION     DASTJP010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14137
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902994"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFQ23_19980"
     CDS             <1..482
                     /locus_tag="VFQ23_19980"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908939.1"
                     /translation="QVSDTPLIMLTALNHEQEMLRGLDAGADDFLSKPFNAEVLLARS
                     KTVLRRTENLRSQNEAFNYNNGYLTIDVERRDVLLNGKRIKLTPVEFRLLTYLARNAG
                     KVLTFEQILSNVWGKEYHGSMDYVHVYISHLRRKLGEDSKSPRYILTLHGVGYIFEK"
     gene            complement(697..3849)
                     /locus_tag="VFQ23_19985"
     CDS             complement(697..3849)
                     /locus_tag="VFQ23_19985"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9908940.1"
                     /translation="MRWIVQSSLRYRYLVIFLAVVLMVFGITRLRNSAVDVFPEFAPP
                     RVEVQTLSMGLSATDVEELVTVPIEQALNGVPGLDVMRSRSVTDLSDVFLIFKPGTDL
                     IYARQLVSERLAAVYPTLPTWAAPPVIIQPLSSTSRAMKIGISSDIYDDMDLSMITYW
                     KIRARLLDVPGVANVPIYGEKIKMPQVQVDLKKMQEYNVTLDEVMEATSDALDVSLLM
                     FSEGSFIGTGGFIDTPNQRLGIRSVLPLITPEKLGEVPIFDRTKEDGSPLLIQDVADI
                     VWGTWPLFGDAVINEGPGLMLIVEKLPWANTLGVTRGVEEALAEMQPGLDNINIDTTI
                     FRPATFIETSINNLTESLLIGAVLVVIILLFFLYEWRVALISATIIPLSLMAALLVLN
                     LQGVAINVMTLAGLAIAIGAVVDDAIVDVENVVRRLRKLRQEGSTQSTASIVLEASLE
                     VRGSIIYASLIEIAALMPVFFLDGLSGAFFRPLATAYVVAGLVSPFIAMTVTPAMVLV
                     LLSNAPIEHRQSPIIPWLHRMYDRVLGQVFRAPAASYVTVGIIVAAGLIVWPRLGQEL
                     LPSFKERDFLMHWILKPGSSHPEMWRITAESCRELLTIPGVRNCGSHIGQAVNADEPY
                     GVYFTENWISVDPSVDYDKTLASVQELVDGYPGLVRDVQTYLKERIREVLTGSSDTVV
                     IRIYGQDLHALGEKADEVRAALEGIPGITDLKKELHMETPQVEVEVDLAKAEQYGLKP
                     GDVRRAAAFLLQGEEVGDIFLNGKTYDVNVWSTPETRHSLSDIENLLIDTPSGERVRL
                     ADVADVRIGAVPNVVEREGQSRKIDVSANVKDRDLGSVAADVESTLAKIDFPLGFHAE
                     VLGEFAERQAAQRNLLTAGSLAVVAIFFLLYTSFGKWRLALLTFFTLPWALVGGLLAA
                     YFSTGVLSLGSLVGLLTILGIATRNGIMMISHFQHLEEEEGMPFGPELVMRGARERIA
                     PIMMTALTTGLALIPLAIAGDLPGHEIEHPMALVILGGLVTSTLLNLLVVPTLYLRFG
                     KAPERADSSLTVSPTPAGTD"
     gene            complement(3868..4284)
                     /locus_tag="VFQ23_19990"
     CDS             complement(3868..4284)
                     /locus_tag="VFQ23_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908941.1"
                     /translation="MKHINRWLVIVIVLVTVVLSACGPSTTSPTKIEPATIEVIEGSD
                     LKQVTLTEKAAERIGLETVAVEAMMVPYAAVIYDIEGNTWVYTNPAPLTFVRAPIEID
                     HIEGDQAFLSQALATDAPVVTVGVIEIYGAETGVSK"
     gene            complement(4320..5744)
                     /locus_tag="VFQ23_19995"
     CDS             complement(4320..5744)
                     /locus_tag="VFQ23_19995"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9908942.1"
                     /translation="MSNLQNIQNVPMRRVNALESAVPAWRLVLIKELADLWIGGKGLI
                     LILAYSLLLGGMAYIASVNAEIDMLPPQEAVSDLLWNSMAVSTFLGLIIGADAFSGER
                     DRGTFESLLLTPASRSHIIIAKQLAGISIWPVAYIIAIPYLVVLAHGSAVLSSALFWG
                     AVTGTILVIGYTGMGMLISFWSGSNKISYFVSLGLYALLLIPAELPGDTVEFVGQFLQ
                     WINPIASTNYFLSNQLDHGTANEFATWLLSSLTLAILSVGLLLGYASKNLRLEAGANI
                     RFWEKLRRQFGLLMIFGLSIVFMSVHPAHALQTEQIKGFVISINNDYYHEVKIGEKIE
                     VGTAITNNTANVSPQLIVAMNVINLDDAGAVVDPEDWSPERTQFIDALKPGQSATLDW
                     VINPIMEGNFMVYMVLIPETANVEATAHPVASPGIHLTVAPFTGFRSSSILPIVIAEP
                     LLLLAITYFIYRRRRQQLDLGGSS"
     gene            complement(5741..6733)
                     /locus_tag="VFQ23_20000"
     CDS             complement(5741..6733)
                     /locus_tag="VFQ23_20000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908943.1"
                     /translation="MSPAPEILVAEGLRKSYGPRCALQGLSFSLRAGHILGFLGPNGA
                     GKTTSIRILTTMMEPDSGHFMVDGISSEYPAKIRQRIGVLPENLGFPKQMTGLEYLTY
                     FGQLYGRTATATRQFALDLLEMVGMHQRANSLIGTYSRGMRQRIGIARALVNDPVIVF
                     LDEPTLGLDLRGRQEFLELVQLIARERNVAVVLCSHLLSEIEGICDDVVILNVGYTVA
                     KGKVAEVIDRVQKNIILRNILRIQVTPESATAAQAVLESIPHIIKIAPIGGLTGYMQL
                     ETVGADNSNSTHIYQINNNILTMLIKAKIPVLRFEVEGGRLEDVFLHLTEDVIR"
     gene            complement(6798..9956)
                     /locus_tag="VFQ23_20005"
     CDS             complement(6798..9956)
                     /locus_tag="VFQ23_20005"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9908944.1"
                     /translation="MMRSIVGSSMKYQFLVITVAIALMAFGVTQFRGMPVDVLPEFSP
                     PYVEIQTEALGLSAEEVEQMITVPIEQDLMAGVAWLDVIRSESVPGLSSVVIYFEPGT
                     DLYRARQMVTERLAQAAIAIPHVSKPPTMIQPLSSQSRFMIVGLSSQELSLIEMSVLA
                     RWTIAPQLMGVPGVAHVAIWGNRDRQLQVLVDPAKLHEQGVSLDQVVDTTGNALWVSS
                     LSYLEASSPGTGGFIDTPNQRLNVWHVLPITSPEELAKVPVDGTEGLRLGDVAQVVED
                     HQVLIGDAVVNESSNLLLVVEKLPGVNTLEVTRALEAELDGLKPGMPGIQFDATLFRP
                     ATFIEMAISNLTRSLVVAALLVVLVLGAFFYGWRAALISMAAILISLFAALFVLYMRG
                     ETLNAMVIAGLAVALGLVIDDAIIDVERITQGLRLNRQQGGLRSSAAVILEAIGETRS
                     ALFFATVITLLAVVPVFVVQGISGSLFQPVAVSYALAVLAAMVVAVTITPALSLILLS
                     KTNLELRESPFTTRLQGGYERNLSRAVKSPGLANIAIVVLVVAAVIAVPFLKRDTTLP
                     AFREPYLMVRFEAAPGTSHPEMNRIVSRATSELRGISGVSNVGAHVGRAVFGDETGNV
                     NSAELWISIDPKANYDATVASIESVVNGYTGLNREVRTYVQQTLSQPQASASDLSVRL
                     YGEDHEILRAEAQKLQQVLAKVNGVADPRVILTSDEPSLEIEVDLAAAQKFGVKPGDV
                     RRTAAILLSGIQVGSLFEEQKVFDVVVWGEPELRQNITNIHDLLIDVPGTDTETGQQV
                     RLGDVAQVRVVSSPTVIHREAVSPYIDIAFTVEGRSVSAVVPDVNAALRSFPYPLEYH
                     AEVVNDYAARQSAQQSIIISSIVAILGIFLLLQASFRSWQLALVSIITLPAALAGAIL
                     ADLLGNGGEISLGLIMGALAVAGIFLRNSMMLVSHYQYLEEQGGESFGADLVMRGARE
                     RMSPTLMTALATGLAFLPFILFGNVPGHEIIRPMALVVIGGLVTSTWFNLFAMPALYL
                     RFGASREADLGFQQATAIAADD"
     gene            complement(10288..11562)
                     /locus_tag="VFQ23_20010"
     CDS             complement(10288..11562)
                     /locus_tag="VFQ23_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908945.1"
                     /translation="MKNLSSIRWIQNLTDQQRDGLLGLFVVLSVAIVYYLQWAYYLTG
                     LEGSKVNVDQPERFFWWANDSRSYRSAGEWLFGREVTNAIGERPWLYPLWLGLARTLF
                     GSNAEGVLWVSQFLMWLASGLFIYLALQNGTKSTIISILGAGVFFSHPSPLILTFHGM
                     TESLNIFLIAMLCWVLTRETDNRYYLAILLLGLATVTKPIYLAVLILLILYVMLRDKQ
                     VSRLKQLGMIALLLAPIWIQLILSTIAIGKPTISTIGAYTFKNYLVADVHLRVEGMEW
                     RESMKEIEDWDLRQQVTYLWDHQRETALAYRRHIIDSNLWTGSFFTLGEGNRLAEFAR
                     TANAIAAYLHLLMLPITVYYLISSKYSRSKETIGLLYLSFALQILTTGISTGQEDRLI
                     ITALPLWIVTYLLVCMGIRESNADSSQKGRLP"
     gene            complement(11663..12556)
                     /locus_tag="VFQ23_20015"
     CDS             complement(11663..12556)
                     /locus_tag="VFQ23_20015"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9908946.1"
                     /translation="MKNYDVLYIGNYTKDTIISPKGINIVDGGAVNYAANAAARLGKK
                     VAVVTRMAKEDEHVVENLEQAGIDCYVTYTPTSTLLKLEYPTADPDIRTLSVMSVAGS
                     ITASDLRQLNAQAAVIGASLRGEVGIDVIRLLKEKGMLVAADMQGFVRVLRGMELRYE
                     PWDEMASTLSLIDVVKSDGVEAEFLTSETDLHKAAEFYAALGPREIVLTHKDGLLIYA
                     DGNFHEMKFYAARLDGRSGRGDTCLGAYVAQRLSMTPGEAGIWAAAVTSLKMENSGPF
                     NRSLYEVEAFILDKYQDHQNR"
     gene            complement(12584..13675)
                     /locus_tag="VFQ23_20020"
     CDS             complement(12584..13675)
                     /locus_tag="VFQ23_20020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9908947.1"
                     /translation="MAYLTLENITKVFPSRGNVGEVVAVQDVNLEIQKGELVTLLGPS
                     GCGKTTTLRMVAGFEFPTSGRIILDGLEINSLPPHKRDMSMVFQSYALFPHLTVFENV
                     AYGLNVQRLSKKTIAERVGQVLDLVHLKGYGDRAPGQLSGGQQQRVALARALVMEPKV
                     LLMDEPLSNLDAKLREEMRTEIRRIQKALNITSVYVTHDQIEAMTLSDRIVIMNEGVI
                     EQIGSPVEIYRFPNSRFVANFIGRANFIDGVVQAQNNSTLTVQSLGEAITLSNIKREF
                     HHGEPVTLIVRPEMIQIKKTGGLYQGVIRQSVYLGDVIEYAVDVGGVSILGTETDPHI
                     TELFPEGEAVTVGFAEDRIQVLPAEEKAE"
     gene            complement(13701..>14137)
                     /locus_tag="VFQ23_20025"
     CDS             complement(13701..>14137)
                     /locus_tag="VFQ23_20025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9908948.1"
                     /translation="VGGAYIIMAAFIIRSLPASVRAGVASLQQIDPSIEEASNILGGD
                     AQYTFRKVTLPLILPALVAGLIFSFTRHMTSLSAIVFLISAKWRIVTASILSEWEQGG
                     VSIAAAYSTLIILIVLIAIALLNIITNRLLRGREGVDLSQGL"
BASE COUNT         3741 a         3601 c         3431 g         3364 t
ORIGIN      
        1 gacaggtctc agacacccct ttaattatgt taaccgcgct aaaccatgaa caggaaatgt
       61 tgcgcggttt ggatgcgggt gcagatgatt tcctgtcaaa gccttttaat gccgaagttt
      121 tgcttgcccg ctccaaaacc gttttacggc gtactgaaaa tcttcggtct caaaatgaag
      181 cttttaatta caataatggc tacctgacca ttgatgtgga gcggcgcgat gtactgctca
      241 acggtaagag aataaaatta accccggtcg aattccgttt gttgacttac cttgcgcgta
      301 atgcgggtaa ggttctaact tttgaacaga tcctcagtaa tgtttgggga aaagagtacc
      361 acgggagtat ggattacgtt cacgtttata tttcacatct ccgccggaag cttggagaag
      421 attccaaaag cccgcgttat attctgacgc ttcatggtgt tggatatata tttgagaagt
      481 aagggcggta tttctaatta taaaatagta ttgtctgtgg caaccatgcg gggttgaaat
      541 atggcagatt ctcaattgtg catcatttga tttcgacatt acctccccta tcctaaacca
      601 tcacttcaga aaaaatccat taacgcgaca cacggtatgg taaaaaccat accgtgtgtc
      661 aatttttgtt tagaatgagc tcttaactat gtgtgattag tctgtgcctg ctggcgtggg
      721 ggacactgta aggctgctat cagcacgctc tggcgctttc ccaaagcgga ggtataacgt
      781 gggcacaacc aacaggttca gcagcgtgga agtaaccaag cctcccaaaa tgacgagcgc
      841 catgggatgc tcgatttcat gaccgggcag atcacccgcg attgcgagag gtatcaaggc
      901 cagaccagtt gttagcgctg tcatcataat gggcgcaatg cgttctcgag cgccgcgcat
      961 gaccagttct ggaccaaacg gcataccttc ttcttcttcc aaatgctgga agtgactgat
     1021 catcatgatc ccattacgtg tggcaatacc cagaatggtg agtagcccaa cgagtgaacc
     1081 cagggacagc acaccggtgc taaagtaagc tgcgagtaag ccgcctacga gtgcccaagg
     1141 gagggtgaag aaggtcaaca gagccagacg ccatttcccg aaggatgtat aaagcaagaa
     1201 gaaaatcgca accaccgcca gggaaccggc tgtaagcaga tttctttggg ctgcctgtcg
     1261 ttcggcaaac tctcccagca cttccgcatg gaagcctagt gggaaatcga tcttggccag
     1321 agttgattca acatcggcgg ccacagaacc cagatcacga tcttttacgt tggcagaaac
     1381 atcgatcttg cgagattgac cttcacgctc gaccacattc gggacggctc caatgcgaac
     1441 gtctgccaca tctgcaaggc gcacgcgttc accgctgggt gtatcgatca gaaggttttc
     1501 gatatcgctc agactgtggc gtgtttcagg cgtactccaa acgttcacat cataggtctt
     1561 tccgttgagg aagatatcgc caacctcttc accctgcagt aagaacgctg ctgcacggcg
     1621 tacatcgcct ggttttagtc catattgttc agccttggcg agatccactt cgacttcgac
     1681 ctgaggcgtt tccatgtgga gttctttctt gaggtctgtg attccaggaa tgccttccag
     1741 agctgcccgc acttcatcgg ccttttcgcc aagggcatgc agatcctgac catagattct
     1801 aattacgacc gtatcacttg accctgtaag aacttcgcgg attctttctt ttagataggt
     1861 ctgtacgtca cgcacgagcc ctgggtaccc atccactaat tcctggacgc tcgccagtgt
     1921 tttatcatag tcaacagagg gatcaacact gatccagttt tcggtgaagt aaacaccgta
     1981 gggttcatct gcattgacgg cctgaccgat atgggatccg cagtttcgta caccgggaat
     2041 ggtcaagagt tcgcgacaag actcggcggt aatgcgccac atttcagggt gcgaacttcc
     2101 aggtttaaga atccagtgca tcaggaagtc gcgttctttg aacgagggca ataactcctg
     2161 cccaagtctt ggccacacta tgagccctgc cgccacgatt atgccaacgg taacgtatga
     2221 cgcagcaggc gcacggaaga cctgtccgag gactcgatca tacattctgt gtaaccaggg
     2281 gatgatcgga gattgacgat gttcgatcgg tgcgttggat aaaagcacca ggaccattgc
     2341 tggtgtaacg gtcatagcaa tgaagggaga caccagtcca gctaccacgt aggcggtcgc
     2401 cagaggccgg aagaacgcac ccgataatcc atccaggaaa aagaccggca tcagggctgc
     2461 gatctcaatg agtgaagcat agatgatcga gccgcgcact tcgagggaag cctccagcac
     2521 aatgctggcc gtcgactgcg tgctgccttc ctggcgaagc ttgcgtagac gacgtacaac
     2581 gttttcaaca tctacaattg catcgtctac cacggctcca atcgcaattg ccaaccccgc
     2641 cagggtcatt acattgattg ccactccctg caggtttagt acaagcaagg ctgccatcag
     2701 agataagggg atgatcgtgg cactgatcag tgcaacccgc cattcataca ggaagaagag
     2761 caggattatc accacaagaa ctgcaccgat gagcagcgac tcagtgagat tgttaatgga
     2821 tgtttcgatg aaggtggccg gccggaaaat ggtagtatca atattaatat tatcaagtcc
     2881 gggttgcatt tctgccaggg cttcttcgac gccgcgggtc acacccaggg tatttgccca
     2941 gggaagcttc tcgacgatca acatcaaacc aggtccctcg ttgatgactg cgtcaccaaa
     3001 gagcggccag gttccccaaa cgatgtctgc tacatcctgt atgaggagag gagatccatc
     3061 ttccttggtt cgatcaaaga taggtacctc gcccaatttt tcgggggtga tgagcggcag
     3121 aacagagcga atgccgagcc gttgattggg tgtgtcgatg aagccgccag taccgatgaa
     3181 tgagccttca gaaaacatca ataagctgac atccagggca tccgaagtgg cttccatcac
     3241 ctcatcaagg gtgacattat attcctgcat ctttttcaga tccacctgta cttgtggcat
     3301 cttgatcttt tcgccataga taggcacgtt ggcaacccct ggcacgtcca ataggcgggc
     3361 acggatcttc cagtaagtga tcatggatag atccatatca tcataaatat ctgatgaaat
     3421 accgatcttc atcgcacggc ttgtcgacga caagggttga atgatcaccg gcggagcagc
     3481 ccaggtaggc aatgtggggt agacggccgc aaggcgctct gatactaatt gccgtgcgta
     3541 aattaagtcg gttccaggct tgaagatcag gaagacatca gaaagatccg ttactgacct
     3601 ggagcgcatc acatctaacc caggaacccc attgagtgcc tgctcgatcg gaacggtgac
     3661 cagttcttcc acatcggttg ccgatagacc catggaaagc gtttgaactt ccactcgggg
     3721 cggagcaaac tcggggaaga catctaccgc ggagtttctc aaccgtgtga tgccaaagac
     3781 catcagcacg acagcgagaa agatcaccag gtagcgatag cgtaaacttg attgaacaat
     3841 ccagcgcata gtgcacctcc tggtgcgtta tttggatact ccggtctcag caccgtagat
     3901 ttcgattaca ccaactgtca caacgggagc gtctgttgcc agcgcctgag aaagaaacgc
     3961 ctgatcaccc tcgatgtggt caatttcaat cggagctcga acgaaggtca aaggagctgg
     4021 atttgtgtat acccaggtat tgccctcgat gtcatagatt acggcggcgt agggaaccat
     4081 catggcctca accgcgaccg tttcgagtcc gatccgttca gctgcctttt cggtcagggt
     4141 cacctgtttg agatctgacc cttcaataac ctcgatcgta gcgggttcaa ttttcgtcgg
     4201 tgatgtggta cttggcccac aggctgaaag aaccactgtt accaggacga ttacaataac
     4261 taaccaacga ttgatgtgtt tcattagatc ctccaaacaa aagaaataat ccagcagggt
     4321 taggatgaac cgcccagatc gagctgctgg cgccgacggc ggtatatgaa atacgtaatt
     4381 gccaataaga ggagaggttc tgctatcaca attggcaaaa tgctagacga acgaaaacct
     4441 gtaaatggtg caacggtcag atgaatcccg gggcttgcaa ccgggtgagc tgttgcctct
     4501 acattggctg tctcggggat gagcaccatg tacaccatga aattgccttc catgatgggg
     4561 ttgataaccc agtcgagggt ggcagattgc ccgggtttca gggcatcaat aaactgggtt
     4621 ctttcaggtg accaatcttc aggatcaacg actgcgcccg catcatccaa attgatcaca
     4681 ttcatagcca cgatcaattg tggagagaca ttggcggtat tgttcgtaat tgctgtacca
     4741 acctcgatct tctctccgat ctttacttca tgatagtaat cattgttgat agagatgaca
     4801 aatcccttga tttgttctgt ctgaagggca tgggctggat gtacagacat aaaaacaatg
     4861 gacagcccaa agatcatcaa aagaccaaac tggcgcctca atttttccca aaatctgata
     4921 ttcgcgccag cttcgaggcg gaggttttta cttgcatacc caaggagcaa cccaacagac
     4981 agtatggcaa gcgtcaatga agacaggagc caggtagcaa attcattcgc tgtcccatga
     5041 tcaagttgat ttgagaggaa atagttcgtc gaggcgatgg ggttgatcca ttgtagaaac
     5101 tgccctacaa attcgacggt gtctcctggt aattctgcgg ggatcagaag aagggcgtaa
     5161 aggccaaggc tgacaaaata gctgattttg ttggagcctg accaaaagct aattaacatt
     5221 cccattcccg tgtacccaat cacaaggatg gttccagtga cggcacccca aaacagtgcg
     5281 ctactcaaaa cagcactgcc atgtgccagc acaaccaaat aggggatagc gataatgtag
     5341 gccactggcc aaatcgaaat gccagccagc tgttttgcaa tgatgatatg actgcggctg
     5401 gccggagtca gcagaaggga ttcaaatgtc ccacggtctc tctcgccgct aaaagcgtcg
     5461 gcaccgataa tcaatccaag gaacgtgctt actgccatgg agttccacaa caaatcagag
     5521 acggcttcct gaggcggcag catatcgatc tcagcgttca ccgaagctat gtaagccatg
     5581 cctcctagca gaaggctgta ggccaagatc aggattaatc cttttccgcc gatccataga
     5641 tctgccagct ccttgatcag aaccaaccgc caggcgggca cagcagactc taaagcgttc
     5701 acccggcgca tcggtacatt ttgaatattc tgcaggttgc tcatcggatc acatcctcgg
     5761 ttagatgcaa aaagacatct tcaagccgcc cgccttcgac ttcgaaccgc aacacaggaa
     5821 ttttggcttt gatgagcatg gtcagaatat tattgtttat ctgataaatg tgtgtggaat
     5881 tgctattatc tgctccgacg gtttccaact gcatgtatcc ggtgagtccg ccgatgggag
     5941 ctattttaat aatgtgaggg atggattcca gcactgcctg cgcggccgtg gctgattcgg
     6001 gggttacctg aatccgcagg atgttccgga gaataatatt tttttggacc cggtcaatga
     6061 cctcggctac tttacctttt gcgacggtgt aaccaacatt caagataacc acatcgtcac
     6121 aaattccttc gatctctgat aggagatgac tgcaaaggac aacagcgaca tttcgttctc
     6181 gggcgatgag ctgcaccagt tcaagaaatt cctggcgccc tcgtagatcc aaacccagag
     6241 taggttcatc gaggaataca attactggat catttacgag ggcgcgtgca atgccgatgc
     6301 gttgtctcat tccgcgactg tatgtgccaa tcaaagaatt cgccctttga tgcataccga
     6361 ccatttcaag gaggtcaagg gcaaactggc gagtagcggt cgcggttcgt ccgtatagtt
     6421 gtccgaagta ggtcaagtat tccaagcctg tcatttgctt gggaaaacct aggttctctg
     6481 gaagcactcc gattctctgg cggattttcg ccgggtattc agagcttatt ccgtcgacca
     6541 taaagtgacc ggagtctggt tccatcatag tagtcaggat acgaatcgag gtcgtttttc
     6601 ctgctccatt tggtcccagg aaaccaagga tatgcccggc cctcagggag aaggataatc
     6661 cctgaagagc gcatctcggc ccataagact tgcgcaggcc ttccgccacc aatatttcag
     6721 gtgccgggct cattggtttg aaatctcttt gctgaatcac tttttacaat ccttcttttc
     6781 agcaccgtga tataggtcta gtcgtctgcg gcgatggcgg tggcttgctg gaagccaaga
     6841 tcggcttcac ggctggcgcc aaagcgcagg tacaaggctg gcatcgcgaa caaattgaac
     6901 caggttgaag ttaccagacc accgataact accagtgcca tcggacggat gatctcatgc
     6961 ccgggtacat ttccgaagag gatgaagggc aggaaagcca agccggttgc cagggcggtc
     7021 atcagagtgg gtgacattcg ctcgcgtgcg ccgcgcatca ccagatctgc accaaagctc
     7081 tcaccacctt gttcctcgag atattgatag tggctgacca gcatcatgct gttgcggagg
     7141 aagatacctg ctacagccag ggcacccatg attaaaccga gagatatctc accaccattc
     7201 cctaagaggt ctgccaaaat agcacccgcc aaagctgcag gcagtgtgat gatggaaacg
     7261 agagccagct gccagcttct gaaggaggcc tgcaagagca ggaagatccc gagaatggca
     7321 acgatactgg aaataatgat gctctgctga gcagactgcc gcgctgcgta atcattcaca
     7381 acttcggcat gatactccag agggtatgga aagctccgaa gggctgcgtt cacatccgga
     7441 actacagcgc ttacgctgcg accttcgacg gtaaaggcga tatcgatgta tggggaaact
     7501 gcctcgcgat ggattacagt gggggaggaa acaactcgca cttgagcaac atctcccagc
     7561 cgaacttgct gacctgtttc agtatcggtg ccgggaacgt cgataagcag atcgtgaata
     7621 ttcgtaatat tttggcgtaa ctcaggctcg ccccagacca cgacatcaaa tactttttgt
     7681 tcctcgaaca ggctacccac ctgaatgcca gataatagga ttgccgcagt ccggcgaaca
     7741 tcgcccggtt tgacgccaaa cttttgcgcc gcagccagat caacctcaat ttccaatgaa
     7801 ggttcatcag aagtgaggat gacacgtggg tcagcaactc catttacttt cgccaaaacc
     7861 tgctgcaatt tttgtgcttc agctcgcagg atttcatgat cctcgccgta tagacgcacc
     7921 gagagatcgg atgcgctcgc ctgcggctgg ctaagggtct gttgtacata ggtgcggact
     7981 tcgcgattca gtccggtgta tccgtttacg acgctctcaa tggaggcaac tgtagcatca
     8041 tagttagctt tggggtcaat gctgatccaa agttctgcag aattgacgtt gccagtttca
     8101 tctccaaata ccgctcgacc tacgtgtgcg cccacgttac tcacgccgga aatgccgcgc
     8161 aactcactgg tggctctgga cacaatacgg ttcatctcgg ggtgcgaagt gccaggggca
     8221 gcttcaaagc gaaccatgag atagggctcc ctgaaggcag gaagcgttgt atcgcgtttc
     8281 aagaacggaa ctgcgataac tgcagccaca accagaacca caatagctat gttagcaagc
     8341 ccgggactct tgacagcccg agacaaattg cgttcgtaac caccctggag cctggtcgta
     8401 aatggtgatt cacgtaactc aagatttgtt ttggataaca ggatcaaact cagagctggt
     8461 gtgatcgtca cagctacaac cattgcagcc agcacggcca gcgcgtatga aacagccacc
     8521 ggctggaaca atgagccgga aataccctga accacaaaga ccggcacaac cgccagtaga
     8581 gtaatcacgg tggcgaagaa taatgcacta cgtgtttcgc caatggcttc aagaattact
     8641 gcagccgatg atcttaaacc gccctgttgg cgattcagtc gtagaccctg tgtgatccgt
     8701 tccacatcaa tgatcgcatc gtcgatcaca aggccaaggg caactgccaa acctgcgatc
     8761 accattgcat tgagagtctc tccccgcata tagagcacaa acagtgcagc gaatagcgaa
     8821 attaaaatcg ccgccatgct gatcaatgca gcgcgccacc cataaaagaa cgcacccagc
     8881 accagaacca caagtaacgc ggcgactacc aacgatctcg tgagatttga gattgccatc
     8941 tcaatgaagg ttgccgggcg gaacagcgtc gcatcaaatt ggatgcctgg catgccgggt
     9001 ttcaatccat ccaactcagc ttccaaggcg cgggtcactt cgagagtatt gactccgggt
     9061 aacttttcaa ctaccagcag aagattggag ctttcgttga cgacagcatc cccgatcaat
     9121 acctgatgat cttccacgac ctgtgctaca tcaccaagac gcaggccttc cgttccatca
     9181 accggtactt tggccagctc ttcaggggaa gtgatcggca aaacatgcca tacattaagc
     9241 cgctgattgg gggtgtcgat aaacccaccc gtcccagggg atgaagcttc gaggtagctc
     9301 agcgaagaga cccagagcgc attacccgtg gtgtcaacaa cctgatcgag cgaaacgccc
     9361 tgttcatgga gttttgcagg gtcaactaac acctggagtt gacgatcgcg gttaccccag
     9421 atcgccacat gcgctacacc tgggacaccc atcagctgag gggcaatcgt ccagcgtgca
     9481 agcacggaca tttcgatcag agacagctcc tgcgaagaaa gcccaacgat catgaaacgg
     9541 ctctgtgaag acagaggctg gatcattgtc ggtggctttg atacatgcgg gatggcgatt
     9601 gcagcctgcg caagacgttc agtcaccatc tggcgagcgc ggtaaagatc ggtccctggc
     9661 tcgaagtaga tgactactga cgaaagacct ggcacagact cggaacggat gacatccagc
     9721 catgccacgc cagccatcag atcctgttcg ataggaacag tgatcatctg ctctacttct
     9781 tccgcagaaa gcccaagcgc ctccgtctgg atctcaacgt aaggcggcga gaactctggt
     9841 agcacatcta ccggcattcc gcggaattgg gtgacgccga atgccatcag tgcaatagca
     9901 accgtaatca ccaggaattg atatttcata cttgatccaa caatcgaacg catcatcgta
     9961 aatacctcct aaaaaagttt cggcaaacaa cacattcaac acacagattg aatgcaccct
    10021 ttaagttaaa tagtagcagg gggctcttaa ggactacttg atgaatcctt aatgttttct
    10081 taaggattaa tagaagatta gaatttcagg attaactaat gaagttgttt agacgacaaa
    10141 aaatcccctc aaaagtgctg cgtttgagaa aaaatttcgc tggagtactc attgtttccc
    10201 cactaaaaat tggggagcac catcgcactc gttgttttgg taaccaattt taggtctgga
    10261 atacggaaat gaaggataag tagaggctca gggcaagcgc cccttctgtg aagaatctgc
    10321 atttgattcc cggattccca tacaaaccag caaataagta acaatccaaa gaggcaatgc
    10381 agtaatgatc aaccggtctt cctgccctgt cgaaatgccc gttgtcagaa tttgaagtgc
    10441 aaaagacaga tacaggagcc caatagtctc tttgctcctc gaatacttgg aagaaatcaa
    10501 gtagtagacc gtaatgggca gcatgagcaa gtggaggtac gccgcaatgg cattagcagt
    10561 gcgtgcgaat tcagccagcc gattcccttc gcccagggta aagaaagagc ctgtccataa
    10621 attggagtca atgatatgac gccgatacgc gagggcggtc tctctttgat gatcccataa
    10681 atacgtcact tgttgtcgca ggtcccagtc ttcgatttct ttcatacttt cgcgccactc
    10741 catcccttct actcgaagat ggacatctgc caccaaataa ttcttaaaag tatatgcgcc
    10801 aatcgtggaa attgttggtt ttcctatggc aatggtcgag agaatgagct ggatccagat
    10861 cggcgcaagc agtaaggcga tcataccgag ttgcttcaat cttgagactt gtttgtcgcg
    10921 cagcattaca tagaggatta gaagaatcaa aacagccaga taaatgggct ttgttaccgt
    10981 cgcaagccct aacaatagga tagcaagata ataacgattg tccgtttctc ttgtcaggac
    11041 ccagcagagc atcgcgatca gaaagatgtt caacgattct gtcatcccat gaaatgtaag
    11101 aattagcggc gatgggtgcg aaaagaaaac gcctgctcca agtattgaga tgattgtaga
    11161 ttttgttccg ttttggagtg caagatagat gaagagaccg ctggcaagcc acatcaggaa
    11221 ttgacttacc catagcacgc cttctgcgtt actgccgaac aatgtgcggg ccagcccgag
    11281 ccagagcgga tataaccagg gtcgttcgcc aatcgcgttc gtgacctcac gcccaaatag
    11341 ccattcgcct gcagagcgat aactgcgaga gtcgtttgcc caccagaaaa atcgttctgg
    11401 ttgatctaca ttgaccttgc tgccttcaag acctgttaga taatacgccc actgaaggta
    11461 ataaacgatt gcaaccgata aaacaacaaa cagacccagc aacccatccc gttgttggtc
    11521 tgttagattc tgaatccatc ttatagaaga aagatttttc atttaacttc gaaaattcag
    11581 tcgctgagca ttatagcatt tgaaactgat gtaaacaaaa atgccgctct aatgagagcg
    11641 gcgttgagtc gcttacagtt ttttaacgat tttggtgatc ttgatattta tcaagaatga
    11701 atgcttcaac ctcataaagt gaacggttaa atggccctga attttccatt ttcagactgg
    11761 taaccgccgc cgcccagatc ccggcttcgc ccggcgtcat tgagagtcgt tgtgcaacat
    11821 aggcaccaag acaggtgtcg ccgcgtccgc tccgcccatc aagacgagcg gcataaaact
    11881 tcatctcatg aaaatttcca tccgcgtaaa tgagcaatcc gtctttatgg gtcagaacaa
    11941 tctcccgtgg acccaacgct gcatagaatt cggccgcttt atggagatct gtttcactag
    12001 tcagaaattc tgcctccacc ccatcacttt tgaccacatc aatcagagat aaagtagatg
    12061 ccatttcatc ccacggctca tagcgcaatt ccatgccgcg cagcacccga acgaagccct
    12121 gcatatcggc agccaccagc atgcctttct ctttgagcaa acgaatgaca tcgatgccaa
    12181 cctcaccgcg caggctggca ccaattacag cagcctgtgc attgagttgc ctcaggtcac
    12241 tggccgtgat cgaacctgcc acactcatga cggaaagggt tctgatatcc gggtcagccg
    12301 tgggatattc gagtttcagc aatgtcgatg tgggcgtata ggtcacatag caatcgatgc
    12361 ctgcttgttc taaattttct acaacatgct catcctcttt tgccatgcgg gtgacaaccg
    12421 ccaccttctt tcccagtcgt gcggcagcat tcgccgcata gttgacagca ccaccatcca
    12481 cgatatttat gcctttggga gaaataatcg tatccttggt gtagttccca atatagagaa
    12541 catcatagtt tttcataatt ttatttgtgg aaggctgaat gaattattcg gctttttctt
    12601 ctgcaggtag aacctgaata cgatcctctg caaagccaac cgtaacagct tccccttctg
    12661 gaaagagctc ggtgatatgc gggtctgttt ctgtccccaa aatcgagacc ccgccaacat
    12721 ccaccgcata ttcgatcaca tcgcctagat aaacagattg gcggatgacc ccttgataca
    12781 agccacctgt ttttttaatt tggatcatct ctggacgaac aatcaacgtc actggttcgc
    12841 catggtggaa ctcgcgttta atgttcgaca aagtaatcgc ctcgcccagc gattggacgg
    12901 ttagcgtgga attgttttgt gcctggacga caccatcgat gaaattggca cgaccaataa
    12961 agttggcgac aaaccggctg ttcgggaacc gatagatttc tactggactc ccgatctgtt
    13021 cgatcacacc ttcattcata attacgatcc ggtctgaaag tgtcatcgcc tcgatttgat
    13081 cgtgtgtaac ataaacgctc gtaatattta atgccttttg aatccttcgg atctcggtgc
    13141 gcatctcctc acgcagcttt gcatccaggt tggagagcgg ttcatccatc aataaaacct
    13201 ttggttccat caccagcgca cgcgccaatg ccacgcgttg ctgctggcca cctgataact
    13261 ggcctggtgc acgatctcca taacccttca aatggaccag gtccaatacc tggccaacgc
    13321 gctcagcaat tgttttctta ctgagtctct gtacattcaa tccataagcg acgttttcaa
    13381 aaaccgtaag gtgtggaaat aatgcataac tttgaaagac catggacata tcccgtttat
    13441 ggggcgggag tgaattgatc tcaagaccat ccaaaataat tctaccggaa gttgggaatt
    13501 caaatccggc gaccatgcgt agtgtagtag ttttcccaca acccgatggt cccagcaggg
    13561 tcaccagctc tcctttttga atctccaggt ttacgtcctg aacggcaact acctcaccta
    13621 cattgccccg tgaaggaaag acttttgtga tattttccag cgttaagtac gccatgaaga
    13681 gttcctcatt tctgtagtgc ttacagtccc tgactgagat ccacgccttc ccggccgcga
    13741 agtaagcggt ttgtaattat gtttaatagc gcaatggcga tcagaacaat caggataatc
    13801 aacgtcgaat acgctgcagc aatgctaacg ccgccttgct cccattcact gagaattgaa
    13861 gccgtgacaa tcctccattt tgcgctgatg agaaaaacga tcgcagagag actggtcata
    13921 tgacgggtaa aactaaagat caggcctgcc accagagcag gaagaatcag tggcagcgtg
    13981 actttacgga aagtgtactg cgcgtctcct cccagaatgt tggatgcctc ttctatcgag
    14041 ggatcgattt gttgtagaga agcaactcca gcccgcacgg aggctggcaa gctgcggata
    14101 ataaacgctg ccataataat gtaggcacct ccaacca
//
LOCUS       DASTJP010000685         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1010365, whole genome shotgun sequence.
ACCESSION   DASTJP010000685 DASTJP010000000
VERSION     DASTJP010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010365"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFQ23_20030"
     CDS             complement(<1..489)
                     /locus_tag="VFQ23_20030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9908949.1"
                     /translation="MTLERGALLHKRYRIVEILGQGGMGSVYRAVDENLGVDVAVKEN
                     LFTTDEYARQFRLEAVILANLRHPNLPRVTDHFVIGDQGQYLVMDYIEGEDLRQRMER
                     MGNVTEDDAILIGAAMCDALTYLHTRKPPILHRDIKPGNVKITPDGHIFLVDFGLAKV
                     LHG"
     gene            790..1755
                     /locus_tag="VFQ23_20035"
     CDS             790..1755
                     /locus_tag="VFQ23_20035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9908950.1"
                     /translation="MINHILVPLDSSALAECVLPHAVAIASVMNARVTLLHVLEHPHN
                     GNGTPAVDPVDWHLRKHKSERYLEQIVERLQGSGLRIEYTILEGSPAQSIIEYARNNN
                     VDLITLSTHGNSGLSGWNVSSVVQKILLRSYKSTLLVRAYKPTPADSSRAHYKRLFIG
                     LDRSMRAEYVLPVAMKIAQADKSELMLGTVIEKPQVIDRFPLSERDSELISELVVRNK
                     ETASHYLKQLLAQFSLEGVDLKTDIVVGENAISVLHDMVDEINADLVILVAHGHSGER
                     RWPYGSVTSSFIAYGNTPLMIMQDLPPDEIPHTHAELALKEGKGH"
     gene            1760..>4398
                     /locus_tag="VFQ23_20040"
     CDS             1760..>4398
                     /locus_tag="VFQ23_20040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908951.1"
                     /translation="MLTTAPRPGKAERATRPREQHDELALELAKKLAACHPRRGDFPV
                     TRQLKNFKDFFQSAYQHFEEATKKEVSVSHTAEWLLDNFYVLEQTIHTVEDDLPADYY
                     HRLPKTPDGWPRVHILALAITRQNNARFDIEQVRRFVQNFQETTPLTVGEVWALPLVL
                     RLTVMEALAEGLAAITKLPWDAPPQPELWQKLKPNESDDVLGEAFSLASSEALVVNSI
                     LNLRMLATQDWKTFFESTSALERILQNDPANLYDEMDFETRNHYRSVIEELALGSKVD
                     ETRIASQAIQLAEAGTSPRERHVGYYLIGRGAARLEAQIAYRPQLRGFVTRFIQRHAT
                     GVYLGSISTLTILFTFLLVLYTARAGGNIAQLILAGFLTVLPASSAAIGIVNWLITSL
                     IPPRTLPKLDFQEGVPAEHRTMVVIPSLLATESDATFLIRQIEHHFLANSDPNLFFAL
                     ITDFADAPEKKMPDDDKVVAPARAAIEQLNKKYGRRGYRPFYLFHRERLWNPSEERWM
                     GWERKRGKLEEFNKLLRGETSTTFTIKLGNLNVLSTIRYVITLDADTLLPRESANRLI
                     GTLAHPLNQAEFDSLTDQVIDGYTVLQPRAQVRPSVANQSLFTRVYSGDSVIDLYTRA
                     VSDVYQDLFGEGNYVGKGIYDVDAFQRSLKDRIPENRLLSHDLFEGIHGRCGLVTDVV
                     LFEDYPPHYLTYTDRMHRWVRGDWQLLSWLKAHVPHRTKGTVPNVLSRIDRWKLIDNL
                     RRSLLPPMVLALLLSAWLFLPGSSLAWTLFALSPYLIPIITNIIAEFHHYFSKRQTLT
                     STHPVRLATLRSLFEIIFLPHESLIIFDAIATTLFRMYIQHKKLLEWVTAAHTVKLFG
                     RRLKVRAAWQAMIVAPAFEIALFL"
BASE COUNT         1088 a         1136 c         1068 g         1106 t
ORIGIN      
        1 tccatgcaac acttttgcca gaccaaaatc taccaaaaag atatgtccgt caggtgtgat
       61 cttgacattc cccggtttga tatcacgatg caggatcgga ggtttacgag tatgaagata
      121 ggtgagcgca tcacacatcg ccgcaccgat caaaatagca tcatcttcgg tcacattgcc
      181 catgcgctcc atgcgctggc gtaaatcctc cccctcaata tagtccatta ccagatattg
      241 accctgatca ccgatcacaa aatggtcggt cacgcgtgga agattggggt gacgcaaatt
      301 tgccaggatg accgcttcca gccggaactg ccgggcgtat tcatctgtgg taaacagatt
      361 ctcttttacc gccacgtcta cccccaggtt ttcatcaacc gcgcggtata cggaacccat
      421 gcccccctga ccgagaattt ccacgatgcg atatcttttg tgcagcagtg cgccgcgttc
      481 gagggtcatg tactagtaag gtctcccgaa aattttgccg tagatttggc attataacag
      541 agagacatcc tacaggttgg gcttatctcc taatttgaaa cgaaaaaaca accttgtaag
      601 gtacaggtaa atgactcccg attcgtccct caatcaatca ttgattcctt ttatcgatct
      661 tcgaacttat ccgcttctta tccgactgga gcggatttgt actataatcc tcaatattgg
      721 ggaaataagg ggtctacacc gtaattccat cgcagagaac ttcgttttaa tgtaaagtag
      781 gagaatgtta tgataaatca tattcttgtt ccactggata gctccgcatt agcagagtgt
      841 gtgttgccac atgcagtggc aatcgcctcc gtgatgaacg cgcgggtcac gctcctgcat
      901 gtgttggaac atccgcacaa tggaaatggt acgccggcag ttgacccggt agactggcat
      961 ctgaggaaac ataaatcgga acggtatctg gaacagatcg tggagcgcct gcagggctct
     1021 ggtttaagaa tcgaatacac tattctcgag ggtagcccgg cgcaaagtat tattgaatac
     1081 gcccgcaaca ataatgtcga tctgattact cttagcacac atgggaacag cggcttgagc
     1141 ggatggaatg tcagcagtgt tgtgcagaag atattgctgc gctcttataa atccacgctg
     1201 ctggttcgcg cgtacaagcc cactcccgcg gattcatcca gggctcatta caaacgcctc
     1261 ttcatcgggc tggaccgatc catgcgcgct gagtatgtgt tacctgttgc aatgaagatc
     1321 gcccaggcag acaagtccga actcatgctg ggaaccgtga ttgaaaaacc tcaagtcatt
     1381 gaccgctttc ccctttccga gagagactcc gagctcatta gcgagcttgt ggtaaggaat
     1441 aaggaaacag cctctcacta tcttaagcaa cttctggcgc aattctcttt ggaaggcgtg
     1501 gatctaaaaa cggacatcgt tgttggggaa aatgccatct ctgtgctgca tgacatggta
     1561 gatgaaatca atgctgatct ggtgatactg gtggcgcatg gccattccgg tgagagacgt
     1621 tggccgtatg gaagtgtaac aagcagtttc atcgcctatg gcaacacgcc tttaatgatc
     1681 atgcaggacc tgcccccaga tgaaatccca cacacccatg ccgaactggc attgaaggaa
     1741 ggcaagggac actgatccta tgctcactac tgctcccaga ccaggcaagg cggaacgtgc
     1801 gacaagacct cgcgaacaac atgatgaact ggctctcgag ctggcaaaaa aattagcagc
     1861 ctgccatccg cgccgagggg actttcctgt tactcgtcaa ctgaaaaatt tcaaggattt
     1921 ttttcagtca gcatatcaac acttcgaaga agcaaccaaa aaagaagtgt ctgtttcaca
     1981 tacggccgaa tggctgttag ataactttta cgttctcgaa cagaccatcc acactgtgga
     2041 agatgatctg cccgccgact actatcacag actcccaaaa acgccggacg gttggccacg
     2101 cgttcatatt ctcgcgctcg ccatcacgcg acagaacaac gcccgtttcg acatcgaaca
     2161 agtcaggcga tttgtgcaaa actttcagga gacgacgcct cttaccgttg gtgaagtgtg
     2221 ggcattacct ttagtgctgc gcctgacggt gatggaggct ttggcagaag gtcttgcggc
     2281 aattacaaaa ttaccgtggg acgcacctcc ccaacccgag ttatggcaaa agcttaagcc
     2341 aaatgaatct gatgacgtct taggggaggc cttttctctc gcttcgtctg aagctctcgt
     2401 tgttaactca attttgaacc tgcgcatgtt ggccacgcag gattggaaaa cgttttttga
     2461 atcgacaagc gctctcgagc gaattctaca gaatgatccg gcgaatctct atgatgaaat
     2521 ggattttgaa actcgcaatc attatcgcag cgtcattgag gaactggcgc tcggttcaaa
     2581 agtggatgaa accagaattg cttcgcaggc tattcagctg gccgaggcgg gaacctcacc
     2641 acgcgaacgg cacgttggtt attatctaat aggacgcgga gcagccaggc tggaagctca
     2701 aatcgcgtat cgcccacaat tgcgcggttt tgttacacgt ttcattcaaa ggcatgcgac
     2761 cggggtgtac ctggggagta tttcaacact caccattctg ttcacttttc ttctggtttt
     2821 gtatacagca cgggccggag gaaacattgc ccagctaatt ctggcaggat tcctgaccgt
     2881 actcccagct tcctcggcag cgattgggat tgtcaattgg ctgatcacat ccctcatccc
     2941 gccgcgcacg cttcccaaac ttgattttca agaaggcgta cccgcagaac atcgaacgat
     3001 ggtggtcatt ccctcgttgc tggcgacgga gtcagacgcc acttttttga tacggcagat
     3061 cgagcatcac tttctggcaa acagtgatcc caatctcttc tttgcgttga tcaccgattt
     3121 tgctgacgcg cccgagaaga aaatgcctga cgacgataag gttgttgcgc ctgcccgggc
     3181 cgcgatcgag caattgaaca aaaaatatgg gcgaagaggg taccgtccct tctatttatt
     3241 tcatcgtgag cgcttatgga atccgagcga agaacgctgg atggggtggg aacgcaaacg
     3301 cggcaagttg gaagagttca acaaactgct gcgaggcgag acttcgacga ccttcactat
     3361 caaactcgga aacctgaatg tattgtcaac gatccgttat gtgatcacgc tggatgccga
     3421 cacattattg ccacgcgaaa gcgcaaaccg gttgatcggc acactggctc atccattgaa
     3481 tcaggctgag tttgattccc tgacagacca ggtgatcgat ggttataccg tcttacaacc
     3541 gcgtgcgcag gtgagaccgt ctgtcgcaaa tcagtcgttg ttcacacgcg tctattcagg
     3601 ggactcggtc attgatctgt atacgcgggc ggtgtctgat gtctatcagg atctctttgg
     3661 cgagggcaac tatgttggga aaggcatcta tgacgtcgat gccttccaaa ggagtctgaa
     3721 agaccgtatt ccagagaatc gactccttag ccatgacctt tttgagggca tccatgggcg
     3781 ctgtggtctg gtaaccgatg tcgtcctctt tgaggattac ccccctcact acttaaccta
     3841 tacagaccgc atgcatcgtt gggtgcgcgg cgactggcag cttttgtcct ggttgaaagc
     3901 acatgtacct catcgtacga aaggcacagt tccgaatgta ctttcacgca tcgatcgttg
     3961 gaagttaatc gataacctgc gccgcagttt gctcccgccg atggttttgg ccttactttt
     4021 gagtgcatgg ctgttcctgc ctggttcaag cctggcctgg acgctcttcg ccctctctcc
     4081 ctacttaatt cccattatca caaacatcat tgcggagttc catcattact tttccaagcg
     4141 ccaaaccctg acatctacac acccggtgcg cctggccacg ctgcgttcgc tatttgagat
     4201 catcttcctg ccgcatgagt cgctaataat ttttgatgca atcgcgacaa ccctcttccg
     4261 aatgtatatt cagcataaaa aattgctgga gtgggtgacc gctgcccata ccgttaaact
     4321 ttttggccgg cggttgaaag ttcgagcagc ctggcaggcc atgatcgttg cacctgcctt
     4381 tgaaatcgcc ttgtttct
//
LOCUS       DASTJP010000686        10740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_322237, whole genome shotgun sequence.
ACCESSION   DASTJP010000686 DASTJP010000000
VERSION     DASTJP010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322237"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1674)
                     /locus_tag="VFQ23_20045"
     CDS             complement(103..1674)
                     /locus_tag="VFQ23_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908952.1"
                     /translation="MVSDPLISTDYWTSVQITPQDVEFLHNYLFERETPLTPRELVAV
                     FVNERFRSERLALQRRREAGGKTYLPKESYQVGDAVIFPALDWRHGKVTAIRPGVNAE
                     IGQFDVLTVAMEGGSERLFASGLQQHSLNDQPDTVEDDGIDTEAILHAHGRTLEKKLE
                     AAFRDDDELVRIAGRWFPRALLVDVNVGHLNLAEAVLDMSGGEPLPTAALLKDVALPE
                     GVNPKLAEFSLNLALQEDRRFDEVGPAGEVLWSLHRLEPEEVREVPLFLEYSPVEHDR
                     SLLTPQMLNLEAQLDDELSEITTEPDPDIKEVTLSLIYPHLRTGTLPLSARIRPLFPT
                     AYESPRVRFTLVDGRTKQKMPGWVVREHGYVYGLRDWYKANELIPGSLVRIRRGEHPG
                     EVIIEARTYRATKDWVRTVIVGADGGVVFAMLKQSISAEFNDRMAFAIPSFEAVDQLW
                     LQDRRRSFDKLVADMIHEMSKLTPQGHVHAQELYSAVNIVRRVPPAPLFALLSSKPEF
                     SHVGDLHFRLTELER"
     gene            1788..3143
                     /gene="yqeC"
                     /locus_tag="VFQ23_20050"
     CDS             1788..3143
                     /gene="yqeC"
                     /locus_tag="VFQ23_20050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HET9908953.1"
                     /translation="MMTLSLVRALRVGKSPRIAFIGAGGKTTAMFQLARLLLKENGRS
                     TVIVTATSHLGSWQIPFADVHIIAGSPETIEANRHRLKGIVLITGDLEQERTKPINDQ
                     TLNWLHQYSEVHSLPILIEADGSRQKPLKAWANHEPPIPSFANHVVQVVGMTGIGKPL
                     TEEHVHRSEIFSNISGLRMNDLVSAEAVITVIKSVTAGLKNFPEHARKTVLLNQADTP
                     ELQASANGLVLPLLKAYDSVIIASLKQEAIFAVHEPIAGIILAGGESSRFGAAKQLLD
                     WKGKPFVRAVAMTALEAGLSPVIVVTGANSERVAAALTGLDITIVENADWKSGQASSI
                     TAGIKALRSKTDHIVGGAIFLLTDQPQITSSILRALVQSHAEGLYSIVAPMVMDQRAN
                     PVLFDRDTFSDLLTLEGDVGGRAIFHKHRAEYLPWHDDRLLLDVDTPEMYQRLISDET
                     L"
     gene            3320..4096
                     /locus_tag="VFQ23_20055"
     CDS             3320..4096
                     /locus_tag="VFQ23_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908954.1"
                     /translation="MDDAGMKKIKILICLIFVIAALGTRVKGVYAAPEALTGTVQSVT
                     LETDTNTATTTVLIELLHNENKQTVRVNLDTAISLGLLTLGGDGNPVINEAILGQLIE
                     IDGATIITDEQENQHPVADALATFFSDIAGLDDETIMSVHDEGTGFGVIAQALWLTRK
                     LDGNAEIFLAIIDAKETRDFSSFVLEDGTTPENWGQFKQAVLNGKRGKPSVTLVNNNG
                     NGGGNGNNGIPHNDNGNGKNNNKNNKENHDQRNNRNNSDR"
     gene            complement(4143..4580)
                     /locus_tag="VFQ23_20060"
     CDS             complement(4143..4580)
                     /locus_tag="VFQ23_20060"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9908955.1"
                     /translation="MYIEVASAVTEELHRSLQRLIPQLTTNNTAPGWEELASLIDSKS
                     STLLLARHPGENGEIVGILTLTIYRVPTGIRSIIEDVIVDENLRRQGIGAALLSQAIE
                     LARREGANSITLTSNPRREAANLLYQSMGFKRRETNAYYLNLK"
     gene            complement(4587..5312)
                     /locus_tag="VFQ23_20065"
     CDS             complement(4587..5312)
                     /locus_tag="VFQ23_20065"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9908956.1"
                     /translation="MFEKLPLWVRRALAWSVHLLTGTGAIWGLLTILAIFEGSWRMVI
                     FWIIVAMFVDGVDGMLARLFHVKKYANTIDGGLLDNMIDYLNYVLVAVLIIIKAPNIV
                     PAGFELPIAFLILLTSGYQFTQLDAKTDNESYFFKGFPSVWNFLVLYLLLLGLNPWIN
                     LALLIVCNILIFVPVKYIYPTRNTRLRRLTLGLTYLCGAIGVWGLLQYPDVPQWVISV
                     AIIYSVYYALLSFFPKIGAVKSA"
     gene            complement(5403..8546)
                     /gene="ileS"
                     /locus_tag="VFQ23_20070"
     CDS             complement(5403..8546)
                     /gene="ileS"
                     /locus_tag="VFQ23_20070"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET9908957.1"
                     /translation="MFKPVSPKLNVTSMEDAVLKFWKQAHIFKKTVEQREGAPEYVFY
                     EGPPTANGKPGVHHVLARAFKDMFPRYKIMRGYHVSRRGGWDTHGLPVEIEVEKQLGF
                     NNKQQIEEYGIDKFNELCKKSVFTYIQDWERLTDRIAFWVDLEEAYVTYTNDYIESVW
                     WILKNFWDKDLLYKGYKVVPYCPRCGTPLSDHEVALGYDEASDPSVFVRMPLVDQPDV
                     SLLVWTTTPWTLPGNVAVAAHPDVDYVFIERDHNGTKEKLILAKSLVQKVFGDEEVKV
                     IETLKGKKLNGVKYQPLFTFMPPDKPAHFVVLGNFVTTEDGSGLVHMAPAFGQEDMEM
                     AREHNLPVLMTVLPNGTFVNEVTPWRGVFVKDADPLIIEDLRARGLLFKAETYTHTYP
                     FCWRCGTPLLYYARESWYIRTSAFRDQLVGLNNTIHWVPEHIRTGRFGNWLENNIDWA
                     LSRERYWGTPLPIWECESCSHREGVGSVEELSQKAGRDLSELDLHRPYVDQVHWDCPD
                     CGKQMKRVPDLIDVWFDSGSMPVAQWHYPFENKQKFEEQFPADYICEAVDQTRGWFYS
                     LHAISTLLFDQVSFKNVICLGHIQDGEGRKMSKSLGNIVQPWDVLNEHGADALRWYLY
                     TATPPGQERRFSSELVGEVIRSFTLTLWNVYSFFVTYANLDKPTVTTMPIATSDLDRW
                     LLSELNVLVRNVTEAYENYDVTSGTRPIEAFVEKMSTWYLRRSRRRFWKSESDSDKQA
                     AYSTLYTALVTVAKLLAPAMPFLAEELYQNLVRSTDETAPESVHLAAWPEVLPEMIDE
                     SLNRDMSLVMRLVSLGHSARQKANRKVRQPLAEAAFSVGNVAERKAVESYADLFMDEL
                     NVKQVRLLNSATEAVSHSVKPLPKQLGQKYGNKFPAIQKAILAMSPEEAAHTLLEGKP
                     LNVQAVGESYQILSEEVEVKALAREGFAVAEDGAYVAALVTDLTPELIREGLAREFVR
                     RVQDLRKSADLDVADRIELYLEASAGLRSAIEAHKDYITSETLTSNLVFETPPETAHV
                     VEDQFDNETVKVGLVKK"
     gene            complement(9015..9875)
                     /locus_tag="VFQ23_20075"
     CDS             complement(9015..9875)
                     /locus_tag="VFQ23_20075"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9908958.1"
                     /translation="MNRKNLKLTRRDFLKLSGNLALLTRGGTLYTTVIEPLWLDIETV
                     TLKLPRLPKSFSGFRLVQISDIHVGKQWIPSQLERVVETVISLQPDIVAITGDFVQAS
                     PDTTDEILALTEGPLAALSSQVPVYAVMGNHDHWWDVERVRNSLAKANVMELNNDFYT
                     FIRDGESLHLCGVDDIFERMDDLDGVLSRLPDDGCAILLAHEPDFADTSAVTGRFDLQ
                     ISGHSHGGQVIVPFVGPVVLPPHGRKYPIGLYKVGDMFQYTNRGLGMIFPYVRFMCRP
                     EITIFNLMPA"
     gene            10001..>10740
                     /locus_tag="VFQ23_20080"
     CDS             10001..>10740
                     /locus_tag="VFQ23_20080"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET9908959.1"
                     /translation="MSHMKPLDTPPNNQIMTAARMDVNLSQLPSRFFRHGWQSWTLTS
                     WIDPSDPPLPVRAPQFRAKDEDPLYAFSKNHVSAWVGAVELADDDILLLGALDLGGRV
                     ELDGLVLRGSYESGEGKWLIARGSEAEVFQRYANLLAEKFGKTRFEKPPRVWCSWYSL
                     YKWINEPIIRNTLNSLGDLPFDVFQLDDGWQITHGDWEPTRKFPSGMADLATRIAATG
                     RTPGLWIAPFMVTKLSSIYRDHPDWLLR"
BASE COUNT         2871 a         2682 c         2543 g         2644 t
ORIGIN      
        1 acacatgcca atcgcgttca tttttcttcc accaggcaaa gtcaatgtga aagggagtat
       61 ctactgtcgg tttgacaaga ctaaatcgtt taacttctgc cattatctct cgagttcggt
      121 caggcggaaa tgcaaatcgc caacgtggga aaattcaggt ttactggaaa gcaacgcaaa
      181 caaaggagcc ggaggtacac gtctgacaat atttactgca gaatataact cctgcgcatg
      241 aacatgtcct tgaggagtca attttgacat ctcatgaatc atatcggcca ccagtttatc
      301 aaaggatcgt cttcgatcct gcagccagag ctgatcgacc gcttcgaacg aagggatagc
      361 gaaagccatt cgatcgttga actctgcgga gatggattgt tttaacatcg caaatacgac
      421 gccgccatcg gcaccaacaa taacggtgcg cacccaatct ttcgttgcgc gataggttct
      481 ggcttcgata atcacctcac ccgggtgctc acctcggcga atacgtacca ggctcccagg
      541 gatcaattca tttgctttgt accaatctct taatccataa acataaccgt gttcacgcac
      601 cacccatccc ggcatcttct gttttgttct accatccacc agggtaaaac gaacccgcgg
      661 cgattcataa gcagttggaa acaacgggcg aattcgggca gaaagcggta aggtcccggt
      721 gcggaggtgg ggatagatca gggacagagt cacttccttg atatcaggat ctggttcagt
      781 cgtaatctca cttaactcat catccagttg ggcctcaaga ttgagcattt gtggcgtcaa
      841 caaactgcga tcatgctcaa ccggagaata ttccagaaac agtggtactt cgcggacctc
      901 ttcgggttca agacgatgca acgaccataa tacttcaccg gcagggccca cttcatcaaa
      961 acgtctgtct tcctgcaatg ccagatttaa cgaaaactct gcaagttttg gatttacgcc
     1021 ctcaggcaaa gctacatctt taagcagagc cgcagtcggc aacggctcac caccagacat
     1081 atccaaaact gcttctgcta aattgagatg acctacgttg acatcaacca gcaaagcacg
     1141 cgggaaccaa cgaccagcaa ttcgtacgag ttcgtcgtca tcccgaaatg ctgcttcgag
     1201 tttcttctcc agcgtccgtc cgtgagcatg cagaatggct tctgtgtcaa tgccgtcatc
     1261 ttcaacggta tcaggctgat cgttcagaga atgctgctga aggccagatg caaagagtcg
     1321 ctctgaacca ccctccattg caacagtcag gacatcgaat tgcccgatct cagcattcac
     1381 gccggggcga atagcggtca cttttccatg ccgccaatcc agcgcaggaa agattaccgc
     1441 gtcaccaacc tgataagact ctttgggaag ataggttttg ccaccagctt cacggcgcct
     1501 ttgtagagcg agccgctcag agcggaaccg ctcattgaca aataccgcaa ccaattcacg
     1561 gggtgtcaga ggcgtttcac gttcaaatag gtagttatga agaaattcta cgtcctgtgg
     1621 tgtgatctgg acactggtcc agtaatctgt ggaaataagg gggtcgctta ccattaccat
     1681 gccttgttgt agtctggcac gcgttttttg cgcggcatga ttattatacc ttaccttgta
     1741 attgagacat acatgtaatt cactgaagtg aaagatgtat aatttctatg atgactcttt
     1801 cacttgtgcg cgcattacgc gttggcaaat ctcctcggat cgcttttata ggtgcaggtg
     1861 gaaaaacaac tgcgatgttt caactagcgc gattgctcct gaaagaaaat ggaagatcaa
     1921 cagtgatcgt tactgcgaca tcccatctcg gttcatggca aattcctttc gccgatgtgc
     1981 atattatcgc gggttcgcca gaaacaatcg aagcaaacag acaccgacta aagggtatcg
     2041 tcctgattac aggcgacctg gaacaggaaa gaacgaagcc gattaacgat cagacattaa
     2101 attggcttca ccaatattct gaagtccact ccctacctat cctgatcgaa gccgacggct
     2161 cacgtcaaaa gccattaaaa gcctgggcaa atcatgaacc acccatcccc tcctttgcaa
     2221 atcatgtcgt ccaggttgta ggtatgacag gtatcggaaa gccgctgact gaggaacatg
     2281 tacatcggtc cgagattttt tcaaacatca gcggcttgcg tatgaacgat ctggtgtcag
     2341 ccgaagccgt gatcactgta ataaaaagtg tgacggccgg cttgaaaaat tttccagagc
     2401 acgcgcgcaa aacggtgttg ctcaatcagg cagatacgcc agaattacag gctagtgcaa
     2461 atggactggt acttcctctg cttaaggcct atgattctgt tattatagca agtctgaaac
     2521 aagaagctat tttcgcggtg catgaaccaa tcgctggaat tatccttgcc ggaggcgagt
     2581 catccaggtt tggcgcagca aaacaacttt tggattggaa aggcaaacca tttgttcgtg
     2641 cagttgcgat gaccgcgttg gaggctggtc tttcgccagt catcgttgtg accggtgcaa
     2701 actcggaaag agttgcagct gccctcacag gcttagatat aacgattgtg gaaaatgcag
     2761 attggaaaag tggtcaggca agttcgatca cagcaggaat taaagcccta agatccaaaa
     2821 ctgatcatat cgttggagga gcgatctttc tgctgaccga tcagccacaa attacctctt
     2881 caatcctgcg cgcattggtc cagagccatg ccgaggggtt gtattcaatc gttgcgccga
     2941 tggtcatgga tcagcgcgcc aacccggtct tgttcgatcg tgatactttt tctgatttac
     3001 taacactcga gggagatgtg ggcggacggg ccatatttca taaacaccgt gcggaatacc
     3061 tgccctggca cgatgaccgg ttgttattag atgttgatac cccggaaatg tatcaacggt
     3121 tgatctcaga tgaaacatta tgaggtagtg attagaccga aaaccatgca atgccctaac
     3181 gtgtgaataa gttacacata ttattaatct ggcattattc aatgtagaat agttctggtg
     3241 agctttgcag aattgaaagg tcccgattga gataaaattt gtaggcgatg actgtaatca
     3301 gtagaatgaa gatagatttt tggacgacgc aggtatgaaa aaaataaaaa ttttgatctg
     3361 tctgatattt gttatcgctg ctttagggac gcgcgtgaaa ggggtctatg cagcaccaga
     3421 ggcgctgacg ggaactgtac aaagcgtcac cctggaaacc gataccaaca ccgctacgac
     3481 gacagttctt attgaattgt tgcataatga aaataaacaa actgtccgtg tcaaccttga
     3541 cactgccatt tcactcggcc tgttaaccct cggcggagac ggaaacccag tgattaatga
     3601 agccatcctg gggcagttaa ttgaaatcga tggagcaaca attatcaccg atgaacagga
     3661 aaatcagcac ccagtagctg acgctcttgc cacgttcttc tcagacatcg ccgggctgga
     3721 tgatgaaacc atcatgtcag tacatgatga gggaacgggt tttggtgtca ttgcacaggc
     3781 gctttggctg accagaaaat tagatggtaa cgccgaaatc ttcctggcga tcattgatgc
     3841 gaaagaaacc agggatttta gctcgttcgt cctggaggac ggtactacgc ctgagaactg
     3901 gggacaattc aaacaggccg tcttgaatgg aaagagggga aaaccaagcg tcacgctggt
     3961 gaacaataat ggtaatgggg gcggaaacgg gaacaacggt attccacata acgataatgg
     4021 aaacggtaaa aataataata aaaacaacaa agagaatcat gaccaaagaa ataatagaaa
     4081 taacagtgac cgatagaatt ctttggtcag atcacgaaaa aggcagaaca atctgccttt
     4141 ttttatttta gattgagata gtatgcgttt gtttcccgcc gcttgaaacc cattgactga
     4201 taaagaaggt tggctgcctc gcgccgagga ttggatgtca gcgtaatact attcgcccct
     4261 tctcttcttg caagctcgat tgcctgagat agcagcgccg ctcctatacc ttgcctgcga
     4321 agattttcat ctacaattac atcctcaata atagaccgaa ttcccgttgg gacccgataa
     4381 atggtcagcg tcaatatccc gacaatttca ccgttctcac cagggtggcg agcaagcaac
     4441 agcgtagatg attttgaatc tattaaagac gccaattctt cccaccctgg agcggtgtta
     4501 ttggtagtca gttgcggaat taatctttgt aaggaacgat ggagttcttc ggtaacagcg
     4561 gatgcgactt caatatacat cgaaactcaa gcagatttga ctgcaccaat ttttgggaaa
     4621 aaactcaaaa gggcataata gactgaataa ataattgcga ccgatattac ccattgcggc
     4681 acatctggat attgtagtaa cccccaaacg ccaatggcgc cacaaagata ggtaagaccc
     4741 agtgtgagac gacgtagacg tgtatttcga gtgggataaa tatactttac gggcacaaaa
     4801 attagtatat tacaaacgat caacaaggcc aggttgatcc aggggttgag tcccagtagc
     4861 agaaggtaga ggacgaggaa attccagacc gacgggaagc ccttaaaaaa gtaagactcg
     4921 ttgtccgtct ttgcatccaa ctgtgtgaat tgataaccag aagtcaacag gatcaaaaac
     4981 gcaatgggta gttcaaaccc tgctggcacg atattgggtg ccttaataat gatcaacact
     5041 gcaaccagca cgtaatttag atagtcgatc atattatcca gtaagccccc atcgatagta
     5101 ttagcatatt tcttgacgtg gaacaagcgt gcaagcatcc catccacacc atcgacgaac
     5161 atggcaacaa tgatccagaa aatcaccata cgccagcttc cttcgaagat ggccaggatg
     5221 gtcagaagtc cccaaatggc tcctgttccc gtaaggagat ggacactcca agccagggct
     5281 cgacgaaccc atagagggag tttttcaaac acgctgcacc tcggataata gtgtagtgca
     5341 attattatac cgccttgagc agacctaaat tgaaaggcgc acctttcgat gcgcctttca
     5401 atctattttt tgaccaagcc cacttttact gtttcgttgt caaactgatc ttcgacaacg
     5461 tgagcagttt caggcggcgt ctcaaagaca aggttggacg tgagtgtttc cgaagtaatg
     5521 taatctttat gcgcttcgat ggcggacctc aatccggcag aggcttccag gtacaattcg
     5581 atgcggtcag ccacatccag gtctgcgctc ttgcgcaggt cttggacgcg gcggacgaat
     5641 tcacgtgcca gaccttcccg aatcaactca ggcgtgagat ccgtgaccag agcggctaca
     5701 tatgctccat cttctgcaac tgcgaatcct tctcttgcga gcgctttcac ttctacttct
     5761 tcagagagaa tttgatagct ttctccaact gcctgcacat tcaacggttt accttccaat
     5821 aaggtatgcg cagcttcttc ggggctcatc gccagaatcg ccttttggat agcagggaat
     5881 ttattcccat acttttgacc caactgcttg ggcagcggct ttacgctgtg agaaactgct
     5941 tctgtggcag aattcaacaa tcgcacctgc ttgacgttca actcatccat gaacagatcg
     6001 gcataggatt ctactgcctt ccgttctgcc acattcccta ctgagaacgc cgcttctgcc
     6061 aggggctgtc gaacctttcg attcgccttt tggcgtgctg aatgcccaag tgagacaagg
     6121 cgcatgacca aagacatatc gcggttgaga gattcatcga tcatttcagg cagcacttca
     6181 ggccacgcgg caaggtgaac agactccggt gcggtctcat ccgtggagcg aacaaggttt
     6241 tggtacagct cttccgcaag aaacggcatg gcgggtgcga ggagcttagc aacggtcacc
     6301 agtgcagtat acaaagtgga gtatgcagcc tgcttgtcgg aatccgattc ggatttccag
     6361 aagcggcggc gtgaacgtcg caggtaccac gtggacattt tctccacaaa cgcctcaatt
     6421 gggcgagtgc cgctcgtcac atcataattt tcataggctt ccgtcacgtt acggacaagc
     6481 acattgagtt ctgacaacag ccagcgatca agatcactgg ttgctatggg catcgtagta
     6541 acggtgggtt tgtcgagatt ggcgtaggtc acgaagaatg aataaacatt ccacagcgtg
     6601 agcgtgaaac tgcgtatgac ctcacccacc aattcggaag agaaacgtcg ttcctgtccg
     6661 ggcggcgtgg ccgtatacag ataccagcgt aaggcatccg caccatgctc attgagcaca
     6721 tcccagggtt gaacaatgtt ccccagcgac ttggacatct tgcgtccttc gccatcttga
     6781 atatgaccca gacagatcac gttcttgaac gaaacctggt caaacaacaa cgtgctgatg
     6841 gcatgcagag aatagaacca gccgcgtgtt tgatcaacag cctcgcagat gtaatccgcc
     6901 gggaattgtt cttcaaactt ctgcttgttc tcaaatggat agtgccactg agcgaccggc
     6961 atggaacccg agtcaaacca gacgtcgatc agatctggaa cgcgcttcat ctgctttcca
     7021 caatccgggc aatcccaatg aacctggtca acatacgggc gatgcagatc caattcactg
     7081 aggtcacgcc ctgctttttg cgaaagttct tctacggaac ctacaccttc gcgatgagag
     7141 catgattcgc actcccagat cggcaaaggc gttccccagt atcgctcgcg gcttaacgcc
     7201 cagtcaatat tgttctccag ccagtttcca aagcgcccag tcctgatatg ctcgggcacc
     7261 caatggatgg tgttgttaag ccccaccaat tggtcgcgaa acgcgctcgt gcggatgtac
     7321 caggattctc gcgcataata taacaagggc gtgccacagc gccagcagaa aggataggta
     7381 tgggtatacg tttctgcttt aaacaacaaa ccgcgggcac gcaaatcctc tatgatgagc
     7441 gggtctgcgt ccttgacaaa gacgccacgc cagggagtga cttcattgac gaaggtacca
     7501 ttgggcaata cggtcatgag cacgggcaaa ttatgctccc gagccatttc catgtcttcc
     7561 tgtccaaagg ccggagccat atggaccaac ccgctgccgt cctcggttgt gacaaaattg
     7621 cctagcacca caaagtgcgc aggcttatcg ggtggcatga acgtgaaaag cggctgatat
     7681 tttacgccat tcaacttctt gcctttcagc gtttctatca ccttcacctc ttcatcccca
     7741 aagacctttt gaaccaatga tttggcaagg atcaattttt ctttcgtgcc gttatggtcg
     7801 cgttcgataa agacgtaatc gacatcaggg tgagcagcca ccgcgacgtt tccaggcagg
     7861 gtccagggcg ttgtcgtcca caccagcaag gaaacatcag gctgatccac cagcggcata
     7921 cgaacgaata cggaagggtc actagcctca tcgtagccca gggcaacttc atggtctgac
     7981 agtggcgtgc cacagcgcgg acaatacggc accaccttat atcctttata gagcaggtct
     8041 ttgtcccaga agtttttcag aatccaccag accgactcga tgtaatcgtt ggtatatgtc
     8101 acatacgctt cttcgagatc aacccaaaaa gcgatacggt ccgtgagtct ttcccaatcc
     8161 tgaatatagg taaaaacaga ctttttacaa agctcgttga atttatcgat accgtattcc
     8221 tcgatttgct gtttgttgtt aaagccaagc tgtttttcga cctcgatctc gacaggtaag
     8281 ccatgggtat cccagccgcc gcgccgcgag acgtgatacc cgcgcatgat cttataacgc
     8341 gggaacatat ccttgaaagc gcgcgccaac acatggtgca caccaggctt tccgtttgcg
     8401 gttggcggtc cttcataaaa aacatactca ggtgcgccct cgcgctgctc aaccgttttc
     8461 ttgaagatgt gcgcttgctt ccaaaatttc agcacagcgt cttccatgga cgtgacattg
     8521 agttttggcg atacaggttt gaacatctta gtctcctggt cgattcgttt tgtagtcgat
     8581 actttggtct cgatacgctc gtggccattg ctcgcttctc gaccaaccgt taaaaataaa
     8641 aactctcatc tcaaacagag acgagagcct gagaagccca cgcggtacca ctctgagtcc
     8701 ctcacttaaa gggcgctcag cgaatgacga ttattacatc atcctgtttt gataacgaag
     8761 aacaactccg gctcgcttac tttcattccg ctggaagatt tctcgaccag aagattaatt
     8821 tgggcttgca gctccgagag gattttcgat ttgcttcatt gacctgctct caccgtacca
     8881 ggttcgctga caacgtaggc aaatgtactc gttctcatta acgctgtttg atttgcggat
     8941 tatacataaa ggttccagat tcggcaagca agctacgcgg ggagattggc taagacaaat
     9001 gaacgagcca catcctacgc gggcattaaa ttgaaaatcg taatctcagg tcggcacata
     9061 aagcgaacgt agggaaatat cattcctaaa ccccggttgg tatattggaa catatccccc
     9121 actttataca aaccaatcgg atatttcctg ccatgtggag ggagcacaac aggacccaca
     9181 aatgggacga ttacctgccc accatgggaa tgcccggaga tctgaagatc aaaacgcccg
     9241 gttactgcgc ttgtatcggc aaaatctggt tcatgcgcca gcaggattgc acatccatcg
     9301 tcgggcaatc tactcagtac gccgtccaga tcatccattc tttcaaaaat atcgtccaca
     9361 ccacaaagat gcagcgattc cccatctcgt ataaacgtgt aaaaatcatt atttagttcc
     9421 atcacgttcg cctttgcaag cgagttccgc acccgttcca catcccacca gtgatcgtgg
     9481 ttgcccatca ctgcatacac gggaacctgc gagctaagcg cggctaacgg cccttcagta
     9541 agcgccagaa tttcatccgt cgtgtccggt gaagcctgga caaaatctcc tgtaatcgcc
     9601 acgatatctg gctgtaggga gatgactgtt tcaactacac gttccagttg agaggggatc
     9661 cactgtttgc ccacatgaat gtcactgatc tgcaccaaac gaaacccgga aaatgacttg
     9721 ggcaaacgcg gcaatttcag cgtcacagtt tcaatatcca gccaaagggg ttctatgacg
     9781 gtagtatata gtgttccgcc ccgcgtaaga agtgcgagat ttccgcttaa tttcaggaaa
     9841 tccctgcgcg ttagtttaag attttttctg ttcataatca gattctggcg ttttgcacag
     9901 gtacatgaga ttaggcttaa ctgaattgtt aaccaaatga aatgaatttg tgatgttgct
     9961 gcaaacctgg gtggtaacct atgctcatta taattacgtc atgtctcaca tgaaacctct
    10021 ggatacccct cccaacaatc agattatgac tgccgctcgg atggatgtga atctctctca
    10081 gcttccgagt cgctttttcc gtcacggttg gcaatcctgg acgctgacct cgtggattga
    10141 cccaagtgat cctcctttgc cagtgcgggc accgcaattc cgggcaaagg atgaagatcc
    10201 actctacgcg ttcagcaaga atcacgtcag cgcctgggtg ggtgctgtag agttagccga
    10261 cgatgacatt ttgctgctcg gcgcgcttga cctcggcggg agagtggaac tcgatgggct
    10321 ggtattaaga gggtcctatg aatcgggtga aggaaaatgg ctgattgccc gcggttctga
    10381 agctgaagta tttcaacgat atgcaaacct gctggctgaa aagtttggca agacgcgctt
    10441 tgaaaaaccg ccgcgcgtct ggtgttcctg gtacagtctt tacaagtgga tcaacgagcc
    10501 cattatccga aatacgctta acagtcttgg agatttgcca tttgatgttt ttcaactcga
    10561 cgatggctgg cagatcaccc atggagattg ggagccaacc agaaagttcc cgtctggcat
    10621 ggcagatttg gcaactcgta ttgctgcaac ggggcggaca cccggcctgt ggattgcgcc
    10681 attcatggtc acgaagttat ccagtatcta tcgcgaccac ccggactggc tactccgtga
//
LOCUS       DASTJP010000687         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1130069, whole genome shotgun sequence.
ACCESSION   DASTJP010000687 DASTJP010000000
VERSION     DASTJP010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130069"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..1464
                     /locus_tag="VFQ23_20085"
     CDS             403..1464
                     /locus_tag="VFQ23_20085"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9908960.1"
                     /translation="MNRMRYYWFITVTLLVILACSSTDPDTLPNRPMLGSSSSGDTSN
                     TIPLRILPPAKLSSESLPLQIDAMRAASYPGSDIVIEATLDPGANYSRYYVSYLSEGL
                     RIYAMMTIPNGQKPPTGWPVIIFNHGYIPPNVYRTTERYVAYVDQIASSGYIVFKSDY
                     RGHDRSEGAAGGVYTGPNYSIDVLNAVASMKRSPDADPNRIGMWGHSMGGYITLRSMV
                     VSQDIKVGVIWAGVVARYPDLFTRWNPGARSTIPAPGSWVHSLEQSYGSVGQNPEFWN
                     SISANAYVRDLNRPIQLHHGTADQDVPWEFSQMLYDEMIAANQIAEIYTYEGDNHNIS
                     NYFSLAMQRTIEFFDRYLK"
     gene            1629..2048
                     /locus_tag="VFQ23_20090"
     CDS             1629..2048
                     /locus_tag="VFQ23_20090"
                     /inference="COORDINATES: protein motif:HMM:NF039385.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HET9908961.1"
                     /translation="MSETNMKESIETTILKEGKVKVTSQKVIIGQEAYFISKIISARL
                     HVKEPALFLPVFYMLTAAVCVALVAISNVDDYGHFLEVGLYFGIGAFLFFLLSTKTKY
                     SVRVRSSLGELSLLEGSDRGIAERIVFAINKAIRSQQ"
     gene            2216..>2469
                     /locus_tag="VFQ23_20095"
     CDS             2216..>2469
                     /locus_tag="VFQ23_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9908962.1"
                     /translation="MPAIEVSNLRKTFQTKRKAAGMSGSVRSLFKPKYSAVEAVRSLT
                     FHMEAGELLGFIGPNGAGKSTTIKMLTGILHPTGGEATVLG"
BASE COUNT          644 a          592 c          572 g          661 t
ORIGIN      
        1 gatggtggtc tcctggctac tggtggagcc aatggatcag tacatctttg gaaagttgtg
       61 tatccttaat aaggccgcgt ccattgtaag taaagacgcg gtacgttcgg tctcgtaatt
      121 gagtcttgtt aatttgccaa cttgactcag aacaaatgtt ctaatataat tccacgaagg
      181 tcaaagcaaa aagaccaatt gaatataccc gacatcctgg ctgcccatta gatactttga
      241 accgaaacct tcagccaccc aggatcatcc tgaaattatt gcgctgcctg tttcatttgt
      301 tatctagtta atgatcacgc ctgggctaag ggatggtatg caggcccatc gaaactcttt
      361 ctaacaaata caagtattgc ttttaatata tgagaattga aaatgaatag aatgcgctat
      421 tactggttca tcacggtgac ccttctggtc atcctggcct gttcaagcac cgatccagac
      481 acgttgccga accgacctat gcttggctct tcctcctcag gtgatacatc gaacacgata
      541 ccactgcgaa ttctgcctcc ggcgaaacta tcttcagaat cccttccact tcagatcgat
      601 gcaatgcgcg cagcgagcta ccccggcagc gatattgtga tcgaagccac gttagaccct
      661 ggtgcgaact acagtcgtta ttatgtttct tatctctctg aggggttaag aatttacgcg
      721 atgatgacga ttccaaacgg acaaaaacca cctactggct ggcccgtgat catcttcaat
      781 cacggatata tcccaccgaa tgtctaccgc acaactgaac gatatgtggc ttacgtggat
      841 cagattgcca gcagtggtta tattgttttc aagtcagatt atcgcggtca cgatagatcc
      901 gaaggcgcgg caggtggcgt ctataccggt cccaattatt cgatcgatgt gcttaacgca
      961 gtcgcatcca tgaagcgctc tcccgatgcc gacccaaatc ggatcggtat gtggggacat
     1021 tccatgggtg gttatattac gctgcgatcc atggtagttt ctcaggatat caaagtcggt
     1081 gtgatctggg caggagtcgt cgcgcgttat ccggacttgt ttacacgctg gaatccgggt
     1141 gcgagatcca ctattccagc tcccggctca tgggtgcatt ccctggaaca atcctatggt
     1201 tcggtgggac aaaaccctga attttggaat tccatctcag cgaatgccta cgtgcgcgac
     1261 ctcaaccggc cgatccagtt gcatcatggc accgcagatc aggatgtacc ctgggaattc
     1321 tcacaaatgc tctatgatga aatgatagca gccaatcaga ttgctgagat ttatacctac
     1381 gaaggcgata accataatat ttcaaactac ttcagcctag ccatgcagcg tacgattgaa
     1441 ttctttgatc gatacctaaa ataggagcca gggtggggat taaccctact tgcccggttt
     1501 cgtaattttt attaaactcc ggacgttatt ttcagattgt gcttcctgtt cacagtattc
     1561 gaagccacac agaagtggtc atggtttcaa cctggtatac acttactcat cctgatatct
     1621 ttaattgaat gagcgagaca aacatgaaag agagcataga gaccaccatc ttgaaagagg
     1681 gcaaggtaaa agttaccagt caaaaagtca tcattgggca agaggcatac tttatttcaa
     1741 aaatcatttc ggcgcgattg catgtgaagg aacctgcact atttcttccc gtgttctata
     1801 tgctgacggc agcggtttgt gtagcactgg tcgctatatc gaatgtggat gactacggtc
     1861 actttctgga agtcggactc tactttggta ttggggcatt tttattcttc ttgctttcaa
     1921 caaaaacaaa atacagcgtg cgcgtccgaa gttctctggg tgaattgagt ctgctggagg
     1981 gcagtgacag gggtattgcc gaacgaatcg tttttgcaat taacaaagcc attcgttcgc
     2041 agcaatagcc ttgctttgaa attctcttag tcttagcaca gatgtaaaag agtgggcatg
     2101 ccaatacagg gtagctgatc agctgcgcgg ctactacaac ggataagacc tttccctcgg
     2161 cacaatacct cagcgcaact ggacacttca tcatggctcg ctgtatactc cctttatgcc
     2221 ggccatcgag gtctccaact taaggaaaac attccagacc aaacgaaaag ctgcagggat
     2281 gagtggctct gttagatccc tgttcaagcc taagtacagc gcggttgaag cagttcgcag
     2341 ccttaccttt catatggagg ctggcgaact acttggcttt atcggcccga atggtgcggg
     2401 caaatcaacg accatcaaaa tgctcaccgg cattttgcac ccaacgggtg gtgaagctac
     2461 ggtcctggg
//
LOCUS       DASTJP010000688         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_467585, whole genome shotgun sequence.
ACCESSION   DASTJP010000688 DASTJP010000000
VERSION     DASTJP010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467585"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFQ23_20100"
     CDS             complement(<1..311)
                     /locus_tag="VFQ23_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908963.1"
                     /translation="MKLLIGVLLIIHGLIVAGQFSGSFGSALPKEVQNPSFVSWWPIN
                     MGRSWLFSSLGLDGTLAVYRIGGLLWLAGGILLVAAGLGVFGFIVPPEWWRSLAVGGA
                     A"
     gene            complement(377..748)
                     /locus_tag="VFQ23_20105"
     CDS             complement(377..748)
                     /locus_tag="VFQ23_20105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9908964.1"
                     /translation="MLKTLRILIVDDQQHTRRSLRALLATRFRLIDTREATNGIEAIR
                     CVEECKPDLVLMDVRMPEMDGIEATRIIKRKLVGVPVIVLTMYSEYKAAALAAGANAF
                     LIKGEPVEQLLAVLLATSSAN"
     gene            complement(898..1398)
                     /locus_tag="VFQ23_20110"
     CDS             complement(898..1398)
                     /locus_tag="VFQ23_20110"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9908965.1"
                     /translation="MSDLNTLAQDFLTQKRIAVVGVSDKRDTGCNLAFRKFRENGYQV
                     YAVNPRITTFEGTQCYPDLKAIPEKPDAVFILTSPQVTEQVVQQCVELGVKQVWMHCM
                     LGTKAGLGASMTSVSQDAVHVCYENGITVIPGACPNQFLKPDFGHTLMRIMCRSLGFH
                     KVNGSP"
     gene            complement(1419..1811)
                     /locus_tag="VFQ23_20115"
     CDS             complement(1419..1811)
                     /locus_tag="VFQ23_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908966.1"
                     /translation="MKTIAQPAVITPGILILAVFFAAVFFIGATGRKVPVLSNVRLDI
                     VLLVILGMAICTQGGIGRVAATGQWAHPLSIIGYVLGGLILLIAFAVFMGWKLPLIQT
                     DQQGLIAVAILAGLKVVNAVTHYLLARA"
     gene            complement(1988..2146)
                     /locus_tag="VFQ23_20120"
     CDS             complement(1988..2146)
                     /locus_tag="VFQ23_20120"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9908967.1"
                     /translation="MAARLSVSELTVRTHVSKILSKLHLASRTQAALYALQKGITSLE
                     DVSMLDET"
BASE COUNT          553 a          564 c          514 g          529 t
ORIGIN      
        1 attgcggccc cacccacggc gagactgcgc caccactcgg gtgggacgat gaaaccgaag
       61 actcccaatc ccgctgcgac cagcaggatc ccaccagcta gccagagcag tccaccgatc
      121 cggtagacgg caagtgttcc atccaaaccg agggaggaaa aaagccagga gcgacccatg
      181 ttgatgggcc accagctcac gaaggaggga ttttgaactt cttttggaag ggcggagcca
      241 aaactgccgg aaaattgacc ggctacgatt agaccgtgga tgataagcaa gacaccaatc
      301 aaaagcttca tcgttcccga atcctttctg caagacctga ggaaaccggg ccttatttca
      361 gctcgctcgt ttctgttcaa tttgctgaag atgttgcaag gagcaccgcc agcaattgtt
      421 ccaccggctc gccttttatg agaaaggcat tggcaccagc agccagcgca gcggccttgt
      481 attcgctgta catggttaac acaatcactg gaacccccac cagttttctc ttgatgatac
      541 gagttgcttc gataccgtcc atttctggca ttcgcacatc catcaatact aagtcgggct
      601 tacattcctc tacgcagcga attgcttcga taccgttagt tgcttctcgg gtgtctatca
      661 gtcgaaatct tgttgcaagc agtgccctca gactccgccg tgtatgctgt tgatcgtcca
      721 cgatcagaat acgcaaagtt ttcagcataa aggttctcta tcagattatt gccatagaat
      781 aacaacacaa agcaagagct ccttcatcga cttaagagtt gatggtcaga aatagttgtc
      841 aggctttgat ctccattgtc ctgctttgat caatgttatg tagacttcaa ttctggttca
      901 tgggctgcca ttgaccttgt gaaagccaag ggagcggcac attattcgca tgagtgtgtg
      961 tccaaaatct ggctttaaga attggttagg acaggcgcca ggaatcactg tgatgccatt
     1021 ttcgtagcag acgtgcaccg cgtcttgaga gacactggtc atacttgccc ccagaccagc
     1081 cttggtaccc agcatacagt gcatccagac ttgctttaca ccaagctcca cacattgctg
     1141 gacaacctgc tccgtgacct gagggctagt gaggataaag acggcatcag gcttctcagg
     1201 gatggccttg agatcaggat agcattgcgt gccctcaaac gtggtgatcc gcggattcac
     1261 cgcgtagacc tgatagccgt tctctcgaaa cttcctaaat gccagattgc aacccgtgtc
     1321 acgcttgtcg gagacaccaa ccacagcaat tctcttctgg gtgaggaaat cttgtgccag
     1381 ggtatttaga tcagacatga gaactccttt tgtgggtctc aggcgcgtgc caagagataa
     1441 tgcgtcactg catttacgac cttcaaaccc gccagaatgg cgactgcaat cagcccttgc
     1501 tgatcggttt gaatgagcgg tagtttccac cccatgaaga ctgcaaaagc gataagcagg
     1561 atcaagcctc ccaacacata gccgatgata gagagaggat gtgcccattg tcccgttgcc
     1621 gccacccgtc caataccgcc ctgtgtacag atcgccatcc caagaatcac cagcaaaacg
     1681 atatccagac gtacattgga aaggacaggg acctttctac cagttgcacc gatgaagaat
     1741 acagcggcga aaaatacagc cagaatcaaa atacctggag taatgaccgc gggttgagcg
     1801 attgttttca tttcctatct cctgctggtg atcttcattt gtacttcatt gccccggaga
     1861 ataactcaga gacctctgat agtcatcatt agaaagtggc gttatcaaat actgaaaaag
     1921 accgcgattt cgcggtcttt ttcaactttt gtggtacggt gtctcctaca tttgtaggag
     1981 aggattatca agtttcatcc agcattgaaa catcctccag cgatgtaatg cccttctgta
     2041 atgcatatag cgcggcctga gtgcggctgg caagatgaag cttgcttaga attttgctca
     2101 catgcgtgcg cacggtcagt tcgctcacag agagtctggc tgcgatttcc ttgttgcaac
//
LOCUS       DASTJP010000689        23003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1008931, whole genome shotgun sequence.
ACCESSION   DASTJP010000689 DASTJP010000000
VERSION     DASTJP010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 23003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23003
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008931"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /locus_tag="VFQ23_20125"
     CDS             complement(<1..1851)
                     /locus_tag="VFQ23_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908968.1"
                     /translation="MPPQQKFADKYLLALEQAIKEKPQAGLNGFEQEWNLLDEDLRPL
                     LTVGAGPSQHSFVDYLRAECIPSWQKQFSQLEVFHWMIEWATRPYYSPRGAIYEARLM
                     EASLINALHRAGINFGERLHYWHGNLLFLTDIGHHSIPGEWGIAKRRYLEKCVDLYGD
                     TLATTGIHTNMSLPDPLFAWDFMHLSPTERGDQHLDEFKSEFYITAARLLRAFAALFV
                     ATTASTPMQAEVRAGRAVVTLTEFDSIRNLTFPNPPIIDLPDLYRSYNDYLQISNDLV
                     RRGVRFGNNNWTPIRARSFAEPVERIISTTSDQLVSLYARGLFAVGEATPPEEMALQI
                     EKQNLMARINLPMGRVEIRTDEGGHSLDLDIANLTLKHLLLLRIYSDAKFARGFRYDR
                     EDISRARANETLAAKHGLRAEIENPLTGKPISMRAFLKWTLNEVKSLAQALNLWNDLT
                     PLVEMAEGGRNTAEKIRARFHASRELGENDEVPLNVLKEFFYEHEAQVKADVEHIASN
                     YGSLGADATKIGEFIQRSRDVVRQTPTAPIRFRPRAQAVIEVSYPDKTSEILDLAQQL
                     IRIPSVTACPDERLDEVHRAGSLIDDYLRNAGLETKFFDGKYPAVYATFPS"
     gene            complement(1954..4392)
                     /locus_tag="VFQ23_20130"
     CDS             complement(1954..4392)
                     /locus_tag="VFQ23_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908969.1"
                     /translation="MTNISIAGNVDGNIVVGDNNFVVNNNHGTIIQQQAAPPVQRKSV
                     SPQPPRKPRSFFGRKRELDEVDSLIASKTPVLIRGIDGIGKTSLLKQAANSDSAKSQP
                     DGVIFLEGLDEEGKLLEFGDLTQRLFNALFDTQPGELKVDHASARTYLSNTQPLVVLN
                     SISLPENDLEKLADLFPNAPILLATEGTAESEAYERILLGPLAREDSVNLLQNSTRVD
                     DPETLAKIAMLLENIPAALITVANAIRQKGLNEDEAFERLQAYVPVEKDKTKAALERA
                     FALMFSTLTEEERGMLIQTAAAPGISIDRKWLENVCGGSAVSETLESMEVLQANSPRL
                     RLIPGLRSFLLRGRDVSNERERLLSYLLSELETRWKDFDFIRDELGNLLGLLFWAVAQ
                     GQWINVVKLGRAIDPYLTLNGLWGAWRKTLEGIQTAAGSTKNLALQGWVQHQLGTYEL
                     GMGNLGAARTLLEQAVKIRTQLGDQVGVAYSQHNLQIIIPAIPPASQPANFIPWALGG
                     LAAIVIAAFLIFSNSSRNAAPPTLVPTALAPSEVPAIASPSATSIVTSTLTEAPTATI
                     TLTSTPAPTDTPTVTLTPTYAVLGKVVVNDVAACFHGPSNIYLNKGTRRIAGNTVDLL
                     GRIETDRGIWVHNRFSLPRTDAGDPCWMDARFLDITPEQLMSVKPIDPMNPDEYQLPT
                     NDRNSTLGYIQDPVVTDVLRRGDIVRVGWQFYDVGEGDYPNHNENFYRYLIEAWLCRA
                     GKIVFTPSGWGPYSPEVINGVTVFADLRDEPGCSEPSHARLYLAWAHGYIGPVEIRTW
                     PQHTTIVPTSTPTP"
     gene            complement(4591..5892)
                     /locus_tag="VFQ23_20135"
     CDS             complement(4591..5892)
                     /locus_tag="VFQ23_20135"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9908970.1"
                     /translation="MIPNTTPVFAVDEKNLIPVSDHLGQMTDIPPSRMFIINKSLKVY
                     QEKNPGDKVFDASQGDGGASLPGTPKYILERAAQIQIEHGTAYDMPFGTEAYRKSVIE
                     QYWKLDSSSGWGPANVLGTAGGRDALVKAYQAMLALGYGRQGDLIMVSRVPWISYNWG
                     PYGVGANVLWAPGDPAEGWAYSEEAIRESVKFAESKGRKIAGILITNPDNPTGLTITV
                     EKQISLAKAALEAGVAFVLFDWMYHYVTDESPMDLNSFLTFFTPEERKRLMFLDGITK
                     SLGGSNIRNCHLIADENVIKFILARASHGVIPPFYSLAVAMAAYEMGFAEAARTIIEP
                     TNASRVALKDLLLRNEFQHIIGKGYYAFIHVGEYLKPKGWKDTELLGQYLAENHGIAI
                     VPGAFFSPFGGEWVRFSYATPVERTEGAFKRLVEGLESLQK"
     gene            complement(6209..6493)
                     /locus_tag="VFQ23_20140"
     CDS             complement(6209..6493)
                     /locus_tag="VFQ23_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908971.1"
                     /translation="MEDSVMALRPCVVEYGQCFDKAEFEIVRNSILKHLIAYGALTND
                     RLGLLVENHLKDKFAGSLYRHYEIVRQVLEARGEIRYLSNPGPPLIQIAE"
     gene            complement(6851..9550)
                     /locus_tag="VFQ23_20145"
     CDS             complement(6851..9550)
                     /locus_tag="VFQ23_20145"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9908972.1"
                     /translation="MNRKYLLRAITLLVLLVSFLPASSVSAQPSTAPPADFFQLPWDQ
                     GVAWVAIDGIDNGTKRPLSSSHHFSVGGAIDFAPRSNMVKGEDTSNFWTAAAGPGTVV
                     GISRCHIIIDHRNGWITQYQFLANIQVQLGDQVERNQRLGIIADGVRNPFCPGSQDPN
                     VPHLHFMLRPTIIGATFAGWTVNYLPVLSKTTFVKNGQAVGLFKPLLNVFDAPPTATP
                     TATQTLTTTPSAETPTATPSPTGVNSPTPTLFGPYVSTTVDPLSILINETALATVGLH
                     NVPVEGYTSAEFTCSFSANVVEVSNITVSDLFGSDAAVAINGPQGNSFIVAIAGSNGN
                     KATTSGIVFTFDVKGLQVGDTNLGCEARVSQGDNVLTQLPSARTFLTVLSTEDTPTPN
                     LTPGTPTVLPSACDRAEFIADVNVPPGTVISPATTFTKTWRLKNLGPCTWTTSYSLVF
                     FHGDSMGAPSSMALTQSVEAGETVDISINLTAPNAPGAHRGYWMFRNASGINFGIGPE
                     ANQPWFVDINVSGPTLPPSLTPTPLPSSPTPTITSDVPTATLTPTSNFPPPVSETATA
                     TPIIPGDWLTFTNLLYGFEFRYPPQGVIADGRTDGFARIDLPFEAGTNLREKYVEVIV
                     QEDVVLCQSPLQTPNPGESVTINGISFLKQTGSDAGVGHLHQWVAYSTLRDNVCVSLD
                     FILHSLNPGNFATPPPEFDFAAESAVFEQIVSTYTWLSSSPTATPTIEETVISESPTP
                     TVTSTPVESPTFVASPTATQTSGAVLTGQVIASKVVTVRLFDLDENFVAVVNAEPDGT
                     FSFNLSAGTYTIIATANGFLRAEGQVTLTEGESLTMPVLNLLAGDIDDNGDIDQFDAM
                     TIGMNYNSAFPEAADLNSDGVINVLDLELLARNYRATGPTPWE"
     gene            complement(9599..10567)
                     /locus_tag="VFQ23_20150"
     CDS             complement(9599..10567)
                     /locus_tag="VFQ23_20150"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9908973.1"
                     /translation="MKSLTAWMLALVLTFAVALNVNAQGNESIWLTANSTAYKTGETV
                     SVLLNASSATPIQGFTFQVRYDPDCLKPVNAVSPIQGMNGLPLPQLRGQVDGSYASTT
                     PQTINGVLAEIRFVALGGCQTNLMLESAALAVRNPEGFAAPLSNVLVGERNVALNIDK
                     EVGVPQTDSSISGSILPLDPPPPTYPEIPGWVIGVLGILLLAGLIFGANKLFRKRTVP
                     PKISPVRVQTPMLQVKHGPQAGKSFALNKLPCMIGRDPYSDIFIDDPHVSSRHAKIFT
                     TNTGYYLMDLSGETFINGQAVRKSPAALKSGDVVRLGKSALFVFAS"
     gene            complement(10632..12161)
                     /locus_tag="VFQ23_20155"
     CDS             complement(10632..12161)
                     /locus_tag="VFQ23_20155"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HET9908974.1"
                     /translation="MKSKRLFQIFILLMLLVGMVGSSQPAKAQTNDPIVITRNLSIWD
                     ATFIGFVSSSIYEQWHFEFTEAHDFTITANTVAGDLVPVLILQDSTGAEITRATGSLT
                     STQPTGNYSVQIQPESGSGFYVLTIRENGITPPPSASVTTSVTPSSVNVGETATVTVS
                     LNNVPAEGYTSAEFTCTYDANLLAVSNVSAAGLFGADPVTAFNSAQNGSFIFAIAGSN
                     GNKATTSGTVFTFSVTGLLAGQTSIECTVRVSKGDNVLTSLPSTGAASLTVTGGAPTE
                     TPTAFPTQTPDGSETATPTPDGSETATSTPDGSETATSTPDGSETATATQTPDGSETA
                     TSTPDGSETATSTPDGSATATSTPTPAESPVPTATSLPDGTLTGQVIASKPVTVGLYD
                     ATDTLVTSVTAEPDGTFSLTAPAGTYTVRATADGFLSAEGFATITSTGTETMPTVSLL
                     AGDIDGNDVIDQFDALTLGMNYNGSTPPAADLNNDGVINVLDLEVLAANYRETGPTVW
                     E"
     gene            complement(12573..15143)
                     /gene="leuS"
                     /locus_tag="VFQ23_20160"
     CDS             complement(12573..15143)
                     /gene="leuS"
                     /locus_tag="VFQ23_20160"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET9908975.1"
                     /translation="MKEKSTNNAKSTKAGSESAPKDKAFNPQVFEPKWQAKWEADNLY
                     RSVIDETKPKHYALTMLPYPSGDLHIGHWFSMTPPDARARFKRMQGYNVLFPMGFDAF
                     GLNAENAAIKHNIHPKKWTYQNIERMRTQMRTMGAMFDWEREAVSADPEYYRWTEWFF
                     IQLYKHGLAYRKKAAVDWCPKDNTTLAREQVKGDDRVCERCGTPVIKKELEQWFFKAT
                     KYADELLHYEGIDWPDYIKTSQTNWIGRSEGASVIFKTAPPFSEKMGGDQGGGDAIEI
                     FTTRPDTLWGATFMVMSPEHPLVDKITTPDYQAAVDEYKMQAEKQTDIQREAKDREKT
                     GVFTGGYAIDQVNNERIPIWVADYVLMSYGTGAIMAVPAHDERDFAFARKFGLKVVPV
                     IQPEGVAELNGDTMETAYEHEGVMINSGPFNGTRATNDKGMKNPAIKAVTEWLEERGI
                     GKRDVNYRYRDWLISRQRFWGAPIPMVYCETHGWNPVPEDQLPVLLPDDIEEWKPTGE
                     SPLKFHPTWKNTICPIGGEPAIRETDTMDTFMCSSWYHLRYLSPHNADVAFDDAEYNY
                     WMPVDTYTGGSEHANMHLLYFRFFHKALRDMGIAEGSEPVMQLRNQGMVLAEDNHKMS
                     KSRGNVVAPDVLVQRYGADTVRAYLMFFARWQQGAPWDSHGIEGTARWMRRVWTLFTD
                     TTDSPASASDEVRRNLRRRVHQTLKRVTHDFENFEFNTIISSLMELMNEMYKAREAGV
                     VGTPEWDEAQEIYLKMLAPVAPHISEELWTNQLGKPYSIHQQLWPTVDDAAAKEDTIE
                     IPVQINGKVRDRITVASDASEDEIKSAALASEVVKKYLEGTEPKKVIVAQKRLVSIVM
                     "
     gene            15891..19835
                     /locus_tag="VFQ23_20165"
     CDS             15891..19835
                     /locus_tag="VFQ23_20165"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9908976.1"
                     /translation="MQFQTNPYLIWQLLPGIVLISIGLYIQSRPVKKRESNNFSLMMF
                     AGSLWAFANAIQLITPNPTWQRFWHAVTYVAIMTVPTSWFLLSTKLTNFFPERIDKIE
                     KYLWYPPAVFYLFLITSGWHGLFFTATNVVSVGGYVSLEDEYGILFFLHTAYSYALLV
                     AGIIILSISLVTNFKKYGLQAYGLIIGVLAPLAGNAYFLFGSPPEGFPDPTPIIFTVT
                     GIAFAWAIFGGHILEVVPLAHEAIVRQLSTGVMVLDGDKNIRDINNAALEMLELPSRT
                     YSGASLMELVQQNMDVALVVNDALVSSAQEDKNIQVAFPKTRRTFDVHISHLGDNLDN
                     TTGWLIQFTDISEKRQAQAELATARETLESVLDSLQDSYFEADVNGVITYANQALCNR
                     LRLSKAELIGKHFRHITIREKIREIYESFQLVYEMKKSLGPFEYAYRSKDGRVYIAET
                     IVSPIIEDGVVVGTRGVLRDITDRLKVEQEIKEQKDLLDSLLQQSPIAMVINDMQKKI
                     TVVNPAFEKLFGYSSEEAIGKSLDNLLSPTRSEDNGNEFSTLVMKKKETRENRRRRKD
                     GSLVDVEIFTAPFFVGGERFGYLAFYNDISERLEAEANLEKTQSSYFAVLETLQDPYF
                     EVNRSGVYVYVNKAFCDSIGYTREEVLGKFFRFVSSRQSVRSTIKRFAAMFENGKPIP
                     PFDFIYRRKDGQEFPGEIVVSPIIEDGEVFGARGIIRDISLRVEAEEILRQAKEAAES
                     RAGELAAINRVSTTVSHSLDLRDILQSVCYELTNIFPIRNAGIGLLTPDKKKLEIVAF
                     HASDPQEKSVKGLFLPVEGNTSSMEVIRNKRTIVIQDAQSDTRTSSIADLSRQRGTKS
                     IMIVPLLARGEAIGTIGMPANDPNYVFSKNEIELAETIAGQIAAAVDNAQLHEKTEHA
                     LDVAERDLEIGRQIQSGFFPEKLPHIPGWEIAAHFNAARQVAGDFYDAFKIKNSNFSA
                     FVIADVCDKGVGAALFMVLFRSLLRAFSETTITRTDVREKLLNIIVSTNNFIAEYHGK
                     SNMFATLFFGVLDPETGTLYYVNGGHEPPIILDAQGNVTQRLMPTGPAVGMFTEMVFR
                     VEQIDFNEGDMLIGFTDGTTDAKNASGKQFTEDRLLQSVSKPWTSNFSMLYQLNSRLQ
                     EHIGAQNQFDDITLISIRRNSLCDTSLFHTIERPAHLAILGELRNFAEQAAQYSNLVS
                     DDVFAFKLAVDELCANIIQYGFEGQEPGRIALSFEKQMDVARLLIKDNGKYFSPDEAK
                     SPDVQADWDEREIGGLGIYFVKELMDNVTYNKAGDGYNQFVLEKRIGKQSSN"
     gene            19848..20183
                     /locus_tag="VFQ23_20170"
     CDS             19848..20183
                     /locus_tag="VFQ23_20170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9908977.1"
                     /translation="MEIKDRQVNGVTVVSLMGSIDAMTAPKITEFIHGQIANGNIKLV
                     VDLDGVDYTSSAGLRVLLGAIKETRAQGGDIRLAKIQPDVQKVLNLSGFTNILKVFGE
                     LDAAVSSYA"
     gene            20198..21040
                     /locus_tag="VFQ23_20175"
     CDS             20198..21040
                     /locus_tag="VFQ23_20175"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9908978.1"
                     /translation="MSRKIALVIGSGSVKCAAALGLLRVLHREKIEVDMVVAGGGGSI
                     FGSLIALGYNVEEIVETNRLLWTREVTEQPNRLAMLQMLLPKFFRSGEYFHLRDDRVM
                     NERLRAAFGDTTFEKTRIPLFITATDYRSGRQAVISDGHIYAAVRASIALPLIFPPSQ
                     RGEQLLADSYLSDPLPVGVAIREGADIILAMGFESVSKVQRNSFSEYVLHLSGVVSNN
                     LLQASYAFYNLAHHSEVLLIVPQFKSEIHMFDTHKVPEIIKAGEMEGEKIMPQLKHMM
                     EMHG"
     gene            21037..21888
                     /locus_tag="VFQ23_20180"
     CDS             21037..21888
                     /locus_tag="VFQ23_20180"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9908979.1"
                     /translation="MKTSKPKPRIALVIGSGSVKCAAALGLMKVLQRENIAVDLVVGC
                     SGGAIYASLIALGWPVEQATEATLRMWTSELTAKRNRRALLQVALPKFFKFDETFGLI
                     DDSLINSRLKQGFKNATFEDTKIPLYVVATDLYNGEQVVISEGSIADAVRGSVSIPYI
                     FPPHKLNGRYLIDGFQSDPLPVGVAIKEGADIIIAMGFESPYQEQISSLMRYNFQVSS
                     VTSNNLLKTNFAFHNLAHHSEIIPILPEFEHRIKLFDTDKIPYVIEQGEKASEKQLRY
                     IKELLVR"
     gene            21900..22745
                     /locus_tag="VFQ23_20185"
     CDS             21900..22745
                     /locus_tag="VFQ23_20185"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9908980.1"
                     /translation="MTNRRRLGLVIGSGGLKCAAAIGVLQVLEQENIPLNMIVGCSGG
                     AAFGASIAMGLSAEAIIKTFTQAWTRSSTGKFDIRSILKIAVPEWVAFDDKIGILDDG
                     GLVRGLVHAYGADASFAETQIPFHAVATDLHTGEAVVLSDGILVKALRASAGIPILFK
                     PVEWNGRWLIDGGLSNPLPVDVAIQQGADVIIAVGFETPLVQSVASPANFALQMFNIL
                     INQLLSRKFAFYNLAYHSEIIAIVPNFDREIRLKDVNEVPQIIEQGRKETLKHLTHLQ
                     RILFA"
     gene            22774..>23003
                     /locus_tag="VFQ23_20190"
     CDS             22774..>23003
                     /locus_tag="VFQ23_20190"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9908981.1"
                     /translation="MKNNKRIALVIGSGGIKCAAAVGLWRVLQEENIEVESLVGCSGG
                     SLYAALIANDADLVLMQELSSRLWTKEIMQGYT"
BASE COUNT         5557 a         5787 c         6003 g         5656 t
ORIGIN      
        1 agaggggaat gttgcatata cagcagggta tttgccatcg aagaatttgg tttcgaggcc
       61 tgcattgcga agataatcat cgatcaacga acctgcacga tggacctcat ctagtctttc
      121 atcagggcag gctgtcacgg atggaatccg aattagctgt tgagccagat ccagaatctc
      181 ggaagtctta tcgggatagg aaacttcgat cactgcctga gcacgcgggc ggaaacggat
      241 cggcgcagtg ggagtctgac ggaccacatc gcgactacgc tgaatgaatt caccaatctt
      301 cgttgcatct gcgccaaggg aaccatagtt ggaagcgata tgctccacat ctgctttgac
      361 ctgcgcttcg tgttcgtaga aaaattcttt caatacattc agcggcactt cgtcattttc
      421 gccgagttcc cgcgaagcat ggaaacgcgc acggattttc tccgccgtgt tgcgtccgcc
      481 ctctgccatt tcaactagcg gcgttagatc attccataaa ttcagcgcct gagccagaga
      541 cttcacttcg ttaagagtcc acttgaggaa ggcgcgcatc gagatgggtt ttccggtaag
      601 cggattttca atctcggcgc gcaaaccatg tttggcggct agtgtttcgt tggcgcgggc
      661 acgggagata tcttcacggt catatcggaa gccgcgcgca aacttcgcgt cagagtagat
      721 acgtagaagg agaaggtgtt tgagggtcag attggcaatg tccaaatcga gcgaatgtcc
      781 accttcgtcg gtgcgaattt caacgcgtcc catcggcagg ttgatgcgtg ccatcaaatt
      841 ttgtttttcg atttgcagag ccatctcttc gggtggcgtc gcttcaccga cggcgaacaa
      901 gccgcgagcg tagagagaaa ctagctggtc actggtggtg gagatgatcc gctcaaccgg
      961 ttcggcaaaa ctgcgggctc tgatcggagt ccagttgttg ttcccaaagc ggacaccgcg
     1021 gcgcacgagg tcattggaaa tttgcaaata gtcgttgtac gaacggtaga ggtcgggcaa
     1081 atcgataatg ggcgggtttg gaaaggtgag gttgcgaatc gaatcaaact cggtgagggt
     1141 gaccaccgca cgtcccgccc ggacttcggc ctgcatcggc gtggacgcgg tagtcgccac
     1201 gaacagcgcg gcgaaggcgc gcagcagccg cgccgcggtg atatagaact cggatttgaa
     1261 ttcgtcgagg tgctggtcgc cgcgttcggt gggcgagaga tgcatgaagt cccaggcgaa
     1321 gagcgggtca ggcagggaca tgttggtgtg aatgcctgtc gtcgccagtg tatctccata
     1381 gaggtcaacg catttttcga gataccggcg tttggcaatg ccccactcac cagggatgga
     1441 atgatgaccg atatcggtga ggaaaagcag gttcccgtgc cagtaatgca atcgctcccc
     1501 gaagttgatg ccagcgcgat gcagcgcgtt gatgagggaa gcctccatga ggcgcgcttc
     1561 gtagatggcc ccgcgtgggc tgtaatatgg acgagtcgcc cattcgatca tccagtgaaa
     1621 gacctcgagc tggctgaatt gtttttgcca ggagggaatg cactccgcac gcagataatc
     1681 tacaaaagaa tgctggctgg gccctgcgcc cacggtaagc agtggacgca gatcttcatc
     1741 gaggagattc cattcctgtt caaagccgtt gagcccggct tgtggttttt ctttgatggc
     1801 ttgctcaagg gcgaggaggt atttgtcggc gaatttttgt tgaggaggca tgataatctc
     1861 tattaaccta atgaagtatt aatttctttc gcggtaataa acggcgtgac tgtccaataa
     1921 cgagcaataa attctatgcg gctgttaaat ggattacggc gtaggcgttg aagtggggac
     1981 aatcgttgta tgctgcggcc aggttcggat ttccaccggt ccaatataac cgtgtgccca
     2041 tgccagatac aggcgggcat gcgagggctc ggaacaccca ggttcgtccc tgagatcagc
     2101 gaacaccgta accccgttaa taacttccgg gctatatggc ccccagcccg agggggtgaa
     2161 aacgattttc cctgccctgc acagccaggc ctcgatcaag tatcgataaa aattttcatt
     2221 atgattgggg taatcgcctt cgcctacatc atagaattgc cagccaactc tgacaatgtc
     2281 accccggcga aggacatcgg taacaaccgg gtcctgaatg tatcccaggg tggaattgcg
     2341 atcatttgtt ggaagctgat actcatcagg attcatcgga tcaattggct tcacgctcat
     2401 aagctgttcc ggtgtgatgt caagaaatct tgcatccatc caacatggat cgcctgcatc
     2461 ggttcggggc aacgaaaatc tgttatgtac ccatattccc cgatcggtct cgatgcggcc
     2521 cagcaagtcc accgtgttgc cggcaatacg ccgggttccc ttattaagat aaatattgct
     2581 tggcccatga aaacatgcag ctacatcatt gacaactact ttacctaaca ctgcataagt
     2641 cggggttagc gtaacagttg gcgtgtctgt aggagctgga gtggatgtca gcgtaattgt
     2701 ggcagttggg gcctctgtta gagtgctggt tacgatggaa gttgccgaag gggaggcaat
     2761 ggctggcact tcgctcggcg cgagggcagt gggcacgagg gtgggcggtg cggcattcct
     2821 tgatgaatta ctgaagatca ggaaagccgc aataacaatg gcagccagtc cgcccaaggc
     2881 ccatggaatg aaattggcag gttgagaggc aggtggaata gccggaatga tgatttgcag
     2941 gttatgctga gaataagcca cgccgacctg atccccaagc tgcgtgcgga tcttaactgc
     3001 ctgctccaac agcgtacgag cggcgccaag gttccccatc cccaattcat atgtccctaa
     3061 ttgatgctgg acccagccct gcagggcaag gtttttcgtc gaacctgcag cagtttggat
     3121 tccctccagc gtttttcgcc acgcacccca aagtccattc agggtgagat acggatcgat
     3181 ggcgcgtccc agtttcacta cgttgatcca ctgtccctgc gcgactgccc agaaaagcag
     3241 gccgagcaaa ttgcccaatt catcgcgaat gaaatcaaaa tccttccagc gcgtttccaa
     3301 ttcagatagc agataactca acaatcgttc acgttcgttc gacacatctc gtccgcgtaa
     3361 gaggaatgat cgaagtccgg gtatgagccg caaacgaggg ctattggcct gcagcacttc
     3421 catcgactcc aacgtttcgc tgacggcgct tcccccgcac acattctcca gccatttgcg
     3481 atcaatggaa ataccgggtg ctgcggcggt ctgaatcaac atgccgcgtt cttcctccgt
     3541 gagagtggaa aacatcaaag caaaggcacg ttcgagggcg gccttcgttt tatctttctc
     3601 aaccggcaca taagcctgca ggcgttcaaa ggcctcgtct tcattcaaac ccttttggcg
     3661 aatcgcattt gccacggtaa tgagagcagc cggtatgttc tcgaggagca tcgctatctt
     3721 agcgagagtt tccgggtcat cgactcttgt actgttttgc agtaaattta ctgagtcttc
     3781 gcgcgccagt ggccccagca aaattcgttc gtacgcttct gattcagcag tgccttccgt
     3841 agcaagcagg atcggggcgt tcggaaagag gtctgccagt ttttccaaat cgttttcagg
     3901 gagagaaata gagttaagca caaccaaagg ctgggtgttg ctcagatagg tgcgtgcaga
     3961 agcatggtca accttgagtt cccccggttg ggtatcaaag agcgcgttga ataggcgttg
     4021 tgtcaggtcg ccaaattcca ggagttttcc ctcttcgtcc agcccttcca gaaagatcac
     4081 tccatccggc tgggacttcg ccgaatcgct attcgcagct tgttttagca gactggtttt
     4141 tccgatgcca tcgataccac ggataagaac cggcgtttta gacgcaatca gactgtctac
     4201 ttcgtccaat tcacgtttac gtccaaaaaa gctgcgcggc ttacgaggtg gctggggaga
     4261 aacgcttttt cgctgaacgg gcggagcagc ctgttgttgg atgatcgtgc catggttgtt
     4321 gttaaccaca aaattattgt cgccaacaac gatgtttcca tcaacattgc ccgcaattga
     4381 tatattcgtc atgtgattaa tcctctaagc agatgaaagc catctggagg ctgcttcggt
     4441 ttgcaagcgc tttccctgga atacgagaat cgaattccat aattttttgt tcgcctgaag
     4501 gtgcgccgga ctgtcagctc tgcatgcgtg cagagtatac agaaaaacag aggctgtaac
     4561 aagcagattt agcgtgcgct cgtcatccgt ctacttctgc aaactctcca acccttctac
     4621 caggcgcttg aaggcacctt cggttcgttc gacgggcgtg gcataagaga agcgaaccca
     4681 ctccccacca aatggagaga agaacgcgcc agggacgatg gcaataccgt gattttctgc
     4741 gaggtattga cccaggagtt cagtatcctt ccagcctttg ggcttgagat attcgccgac
     4801 gtggatgaag gcataatatc ccttgccgat gatgtgttga aattcatttc tcagcaaaag
     4861 gtcctttaat gctacacggc tggcatttgt tggctcgatg atcgtcctgg cggcttcagc
     4921 gaatcccatc tcgtacgcag ccatcgcaac cgccagcgaa taaaacggcg ggatgactcc
     4981 atgcgaggcg cgcgccagga tgaacttgat aacattttca tccgcgatca aatggcaatt
     5041 acgaatattt gacccaccaa gagatttggt aattccatcc agaaacatta atcgcttgcg
     5101 ttcttcagga gtaaaaaacg tgagaaagga attcaagtcc atcggcgatt cgtcagtcac
     5161 atagtgatac atccaatcga agaggacaaa cgccacgccc gcttccaacg ccgctttcgc
     5221 aagtgagatt tgtttctcga cggtgattgt taaccctgtt gggttatcgg gattggtgat
     5281 aaggatacct gcgatctttc ttccctttga ttcggcgaac ttcacactct cgcgaatcgc
     5341 ctcctcagaa taagcccagc cctctgcggg gtcaccgggt gcccacagca cattggcacc
     5401 gacaccgtat ggtccccagt tgtacgaaat ccatgggacg cgcgagacca tgataaggtc
     5461 tccctggcgt ccataaccca gcgcgagcat cgcctgatag gctttgacca gcgcgtcgcg
     5521 ccctccagcc gtgccgagga cgttcgccgg tccccagccg gaggaggagt ctaacttcca
     5581 atactgctca atgacagatt tgcgataagc ttccgtgcca aagggcatat catacgcggt
     5641 gccatgctca atttgaattt gcgcagcgcg ttcgaggatg tatttcggag tcccgggcaa
     5701 cgacgcgccc ccatcaccct gagaggcatc gaaaacttta tcaccggggt tcttctcctg
     5761 atacaccttc agcgacttgt tgatgatgaa catacgcgag ggtgggatat ctgtcatttg
     5821 acccagatga tcactgacag ggatgagatt cttttcgtca actgcaaaga cgggcgtagt
     5881 gttggggatc atgaatggct ccttgccccc acccatccct tcgggactcc ctccccaaat
     5941 tctagaattt ggggagggtc ggagaggggg taaaataacg ccccgatttg ctcggggcgt
     6001 ctggtgtacc tagtgaggtt gaaccttttg ttgttcatcc gatagccacc aacgtcccgt
     6061 cacaaaatga gtgacagcat tatcattgtt ggtagtggta tcaaggataa acattgggcg
     6121 caaggatacc attgggatat gcttgagtca agcaggattt cggttaaata aaacagcggc
     6181 gcgaagttgc gccactgttt cgaaggaact attctgcgat ctggatgagc ggaggtccgg
     6241 ggttggatag gtaacgaatt tcaccgcgcg cctccaacac ctgccggaca atctcataat
     6301 gccgatacag tgaaccggca aacttgtcct tcaaatggtt ttctaccaac aaaccaagtc
     6361 tatcgttggt cagggcaccg taagcgatca ggtgcttcaa gatcgagttt ctcacaattt
     6421 caaactcagc tttgtcaaag cactgaccat actcgaccac acaggggcgg agcgccataa
     6481 cggaatcttc cattctgcct cacttgtaga aattttatgt agagaaagcg aaaaggagac
     6541 acacatttta gaacactagt tctaatccgt caagcgccag atatagggag tttgtacaac
     6601 aggacaccag atgcatcctc actccctcat caatgcaacc ttatagggat acaaggtcgc
     6661 gctcatcacc cagccgaaga ccgccgagtc agtcgagacc gcagctgctt tttgcggtct
     6721 cgacctctct gcgatgatgc cgaggcgcgt gagtttccga aatgtttgac acacctttag
     6781 caacagaaac gaaaactccc cagcgtcttg cgacgttggg gagttcccgt tcaacttatt
     6841 gagttacagt tcactcccac ggagttggcc ccgttgctcg ataattccga gccagcagtt
     6901 ccagatcaag gacgttgatc actccgtcac tattcaaatc ggccgcctcc ggaaatgcag
     6961 aattgtagtt catcccaatt gtcatcgcgt cgaattgatc gatgtcaccg ttatcgtcta
     7021 tatcccccgc aagcaggttg agcaccggca tagtaagact ctccccttcc gtaagcgtga
     7081 cctgcccctc ggcgcggagg aatccatttg cagtggcgat aatggtatag gttccagccg
     7141 agagattgaa gctgaaggtt ccatctggct ctgcattgac cactgccaca aaattttcat
     7201 ccagatcaaa taagcgaacc gtgactacct tggacgcgat cacctgaccg gtaagcactg
     7261 ctcctgaggt ttgtgtagca gtgggtgaag ccacgaacgt gggcgactca acaggtgttg
     7321 aagtcaccgt tggcgtaggt gactcagata tgactgtttc ctcaatggtc ggcgtagccg
     7381 taggtgaaga agacaaccat gtataggttg acacgatctg ctcaaagaca gccgactcag
     7441 ctgcgaagtc gaattcgggc ggcggtgtag cgaaattacc cggattgagt gaatgcagga
     7501 tgaaatccag gctgacacag acgttatccc gcaacgttga ataggcaacc cactgatgga
     7561 gatgacctac cccggcatca cttcccgtct gtttcaaaaa cgagatcccg tttatagtca
     7621 cactttcacc tggattaggt gtttgtagag ggctttggca aagcactaca tcctcttgaa
     7681 caatcacctc cacgtatttc tcacgaagat ttgtacctgc ctcaaaagga aggtcaatgc
     7741 gggcgaaacc atccgtcctt ccatctgcaa tgactccctg tggcggatat ctgaactcaa
     7801 agccatatag gaggttggta aaggttagcc aatccccagg tatgatggga gttgcagtcg
     7861 ccgtttcgga taccggtggt gggaagtttg acgtgggagt caacgtagct gttggcacat
     7921 cggatgtaat ggtcggcgta ggcgaggatg gaagtggcgt aggtgtgagc gaaggtggga
     7981 gtgttggacc cgacacatta atatctacaa accagggttg atttgcctcc ggaccaatgc
     8041 cgaaatttat tccgctggca tttctgaaca tccaatagcc gcgatgggca cccggcgcgt
     8101 tcggtgcagt caggtttatg gaaatatcca cagtctcgcc cgcttcaacg ctctgggtaa
     8161 gagccatgga tgaaggcgca cccatgctgt ccccatggaa gaatacaaga ctgtacgaag
     8221 tagtccaggt acagggaccg aggttcttca gtcgccaggt ctttgtgaaa gtagtggctg
     8281 gggagattac ggttcccggc ggcacgttca catctgcgat aaattctgct cgatcacacg
     8341 ccgagggtag gacggttggc gtgcctggtg tgagattcgg cgtgggagta tcttctgtgc
     8401 ttagaaccgt caggaaggtg cgagcagacg gaagttgtgt cagtacgtta tcaccttgtg
     8461 aaacgcgtgc ttcacatcca aggtttgtgt cgccgacctg taatcctttc acatcgaaag
     8521 tgaatacgat tccacttgtg gtagctttat tgccgttact gcctgcgatg gccacgatga
     8581 aactgttacc ctgtggccca ttgatggcta ctgcagcatc gctgccaaac aggtcgctca
     8641 cagtgatatt gcttacttcg accacattcg cagaaaagct gcaagtgaac tcagcactgg
     8701 tgtagccttc cacaggtaca ttatgcagtc caacagtagc cagcgctgtt tcattgatga
     8761 gaatagacag gggatcaacg gtcgtcgaaa catacggacc gaacaatgtt ggcgtcggtg
     8821 aatttacccc ggtgggcgaa ggcgttgccg tgggcgtttc ggcgctcggg gttgtcgtca
     8881 aagtctgcgt cgcggtcgga gtggctgtcg gtggggcatc aaatacattc aagagtggtt
     8941 tgaataaccc aacggcttga ccgttcttca caaatgttgt tttgcttaat acggggagat
     9001 aattaacggt ccagccggca aaggtcgctc cgatgatcgt gggccgcaac atgaaatgca
     9061 gatgcggcac atttgggtcc tggcttcccg gacaaaatgg attgcgcacg ccgtcggcga
     9121 taatgccaag gcgctggttg cgttccactt gatcgcccag ctgcacttga atgtttgcca
     9181 ggaactgata ttgagtgatc catccattgc gatgatcaat gatgatatga catcgcgaga
     9241 tgccgaccac cgtccctggt cctgctgcag ctgtccaaaa attggatgta tcttcgccct
     9301 tgaccatatt gctgcgcgga gcgaaatcaa tcgcgccacc caccgaaaaa tgatgcgaac
     9361 tgctgagagg acgctttgtg ccattatcga tcccatcgat cgcgacccat gcaactccct
     9421 gatcccaggg caattggaaa aagtcagcgg gaggcgcggt ggaaggttgc gcgctgacgc
     9481 tactggctgg caaaaaagag accagtagca caagcaatgt aatagcccga agcaaatact
     9541 tacgattcat ggtcatttac tcctctttcc ctttaatggg ctatacctac ggaaccagtt
     9601 atgatgcaaa cacaaacaag gcactttttc ccaggcgcac cacatcaccc gatttgaggg
     9661 cagcagggct tttacgaaca gcctgaccgt tgatgaacgt ctccccgctc agatccatca
     9721 ggtaatagcc agtgtttgtg gtaaagattt tcgcgtggcg gctgctgacg tgagggtcat
     9781 cgataaaaat atcactataa gggtcgcgcc cgatcatgca gggcagtttg ttaagcgcaa
     9841 aacttttccc ggcttgtggt ccgtgtttca cctgtaacat cggagtttga actcggactg
     9901 gacttatttt tgggggaacc gttcgttttc gaaatagttt gttcgctccg aaaatcagtc
     9961 ctgccagaag tagaattccc aggacgccga taacccagcc tgggatttca ggatacgtgg
    10021 gcggaggcgg atcaagtggc aggatcgagc cggaaatgga tgagtccgtt tgaggaactc
    10081 ccacttcctt atcaatattc aaagccacat ttctttcacc aaccagcaca ttcgacaatg
    10141 gagcagcaaa cccctccgga ttgcggacag caagagccgc gctttcaagc atgaggttgg
    10201 tctggcagcc acccagagct acgaatcgga tctcagccaa cacaccatta attgtctgtg
    10261 gtgtggtgct ggcgtatgat ccatccacct gacctcggag ttgagggagc ggcagaccat
    10321 tcatgccctg aatgggactc acagcattga caggtttcag acaatccgga tcgtagcgaa
    10381 cctgaaacgt aaacccttga atgggtgtgg cagacgaagc attcaacagg acggatacgg
    10441 tttctccggt cttatatgcc gtcgaattcg cagtaagcca gatactttca ttaccctgag
    10501 cgttgacatt cagcgcaacc gcgaacgtaa gtacaagcgc gagcatccag gcagtaagtg
    10561 atttcattta acactccagg aaagggacgc acacgttatg acatgtgcgt ccctttatgt
    10621 ttattgcaat actattccca cactgttgga ccggtctcac gatagttagc tgcaagaacc
    10681 tcaaggtcta aaacgttgat cacgccatca ttattcaaat ccgcagcggg gggtgttgac
    10741 ccgttgtagt tcatgccgag ggtcagcgca tcgaactggt cgatcacatc gttgccatca
    10801 atgtcaccgg cgagcaaact tacggtcggc attgtttcag tgccggtgct ggtaattgtg
    10861 gcaaaacctt cagcgctgag gaaaccgtct gctgtagcac ggacggtgta cgtaccggcc
    10921 ggagccgtca ggctgaatgt tccatccggt tcagcagtca ctgaggtgac cagtgtatcg
    10981 gttgcatcgt acagaccaac cgtgacaggt ttactggcaa tgacttgtcc cgtaagagtt
    11041 ccatcgggca aagaggtggc tgttggtaca ggcgattcag ccggggtggg cgtagacgtc
    11101 gctgtagcag atccatctgg cgttgaagtg gctgtttccg agccgtctgg ggtcgaagtc
    11161 gccgtctcag atccatcagg cgtttgcgta gcagtagcag tttcagatcc atccggggta
    11221 gacgtagctg tctcggagcc atcgggggtg gaggtcgctg tttctgaacc atccggtgtt
    11281 ggtgtagcag tctcagatcc atccggtgtt tgtgtgggga aggccgttgg cgtctcagta
    11341 ggtgcaccgc cagtgaccgt caggcttgcg gcaccagtcg agggcaggct tgtgaggacg
    11401 ttatctccct tggaaacacg caccgtacat tcaatggaag tctgcccggc caacaagcct
    11461 gtaacgctaa acgtaaaaac tgttccgctg gttgtggctt tattgccgtt actgcctgca
    11521 atcgcaaaaa taaagctgcc attttgtgcg ctattgaatg cagttaccgg gtcagcacca
    11581 aataatccgg ccgcggatac attactcaca gccagcagat ttgcgtcgta cgtacacgta
    11641 aattcagcgc tggtatagcc ttctgcaggg acattattca aactgacagt cactgtcgcg
    11701 gtctcaccca cattgacact tgaaggtgtg acagaggtgg ttaccgacgc agaaggtggc
    11761 ggggtaatgc cgttttcgcg aatcgttaga acgtaaaagc cactgcctga ttctggttga
    11821 atctgaacgg aatagtttcc agtaggttgc gtgctggtta atgagcccgt ggcacgtgtg
    11881 atttcagctc ctgttgagtc ctgcagaatg agcacaggca ccagatctcc tgccacagtg
    11941 ttcgcagtga tcgtaaaatc gtgagcttcg gtgaactcga aatgccattg ttcgtaaata
    12001 cttgagctca cgaaaccaat gaatgtggca tcccaaatcg ataagttgcg ggtgataaca
    12061 atgggatcat tggtttgagc ctttgcaggc tggctgctcc ctaccatgcc taccaacagc
    12121 atgagcagga tgaagatctg gaacaggcgt ttagatttca tggtcatatt ctccttatca
    12181 aacttgaatt ttgaacaata cgattgcaga caactgatta ctgacgcaaa aaattcaggt
    12241 cgttcggacc tcatcgcacc tccaggcaaa atttgctttc ccgcacagtt atgcggagat
    12301 ggatgtgtta tttgaagcag ataccatttt tatctagaac aagcactggg atttcatgac
    12361 cgctttctca tcagggatga gaagggaata ctcgatttcc attgtatgac tctattgtgg
    12421 attggcacgt tgcagtaaag ttgccacgat attgcaaaat aattgcgaaa ttaaaaattt
    12481 agtaccaggg tatgatacgc aggcttaata aaaagaaagg cgatggattt ctaaagaaat
    12541 ccatcgcctt caagtcaacc tgctgtgatg tcttacatca cgatactcac taatcgcttc
    12601 tgtgccacaa ttactttctt tggctctgtt ccttccagat acttctttac tacttcgctc
    12661 gccagtgcag cagatttgat ttcatcttca cttgcatcag atgctactgt aattcgatca
    12721 cgaactttgc cgttgatctg cacagggatt tcaatcgtat cttctttcgc agcagcgtca
    12781 tccactgtgg gccacaattg ttgatggatg gagtatggct tacctagttg attcgtccac
    12841 aattcttcgg agatatgtgg cgcaacaggg gccagcatct tcaaatagat ttcctgagct
    12901 tcgtcccatt ctggtgtgcc aactacccca gcttcgcgcg ctttgtacat ttcgttcatc
    12961 aattccatca gtgaagaaat gatcgtgttg aactcaaaat tctcgaaatc gtgtgtgacg
    13021 cgcttcaagg tttggtgaac gcgtcggcgc aaattccgcc ggacctcatc agaggccgac
    13081 gcgggtgaat ctgtagtatc tgtaaacaaa gtccatacgc ggcgcatcca gcgcgcggtc
    13141 ccttcgatgc cgtgtgaatc ccacggcgca ccctgctgcc agcgcgcgaa gaacatcaaa
    13201 taggcgcgga cggtatccgc tccataacgc tgaaccaaca catccggtgc gaccacattg
    13261 ccgcggctct tggacatttt gtggttgtcc tccgccagca ccatgccctg gtttcgcaac
    13321 tgcattactg gctcgctgcc ttcggcgatg cccatatcgc gcaatgcctt gtggaagaac
    13381 cgaaaataca gcaagtgcat gttcgcgtgt tcgctgccac ctgtatatgt atccacaggc
    13441 atccaatagt tgtactcggc atcatcgaac gctacatcag cattgtgtgg actcaaataa
    13501 cgcaggtgat accacgacga acacatgaaa gtatccatgg tgtctgtttc acggatggct
    13561 ggttcgccgc cgatcgggca tatcgtgttc ttccatgtcg ggtggaactt caatggactt
    13621 tcacccgtcg gtttccattc ttcaatatca tccggcaaca gcaccggcaa ttgatcttcg
    13681 ggcacaggat tccatccgtg cgtttcgcaa tagaccatcg gaatcggcgc gccccaaaaa
    13741 cgttgccgcg agatcaacca atcccgatac cgataattca catcccgttt gccgatacct
    13801 cgttcctcca gccattcggt cactgctttg atggcgggat tcttcatgcc tttatcattc
    13861 gttgcgcggg tgccattgaa cggtccgctg ttgatcatca caccttcatg ctcataagcc
    13921 gtttccatgg tatcaccatt caactcagca acaccttcag gctggattac tgggaccact
    13981 ttcaaaccaa attttcgagc aaaagcaaaa tcacgttcat catgcgcagg cacagccatg
    14041 atcgcacctg tgccatacga catcaacaca taatcggcaa cccagatcgg gatgcgctca
    14101 ttgttgacct ggtcgattgc gtagccaccg gtaaatacac ccgtcttttc ccgatctttc
    14161 gcttcgcgct ggatatccgt ctgcttctct gcctgcatct tatactcatc tacggcagct
    14221 tgataatcgg gcgtagtgat tttatccact aacggatgct caggcgacat gaccatgaac
    14281 gttgcacccc acaacgtgtc tggccgggtg gtgaaaattt ctatggcatc acccccccct
    14341 tgatcccccc ccattttttc cgaaaatggg ggggctgttt tgaatatcac agacgcgccc
    14401 tcgcttcgac cgatccaatt cgtttgagaa gttttgatgt aatccggcca gtcaatgcct
    14461 tcatagtgca acaattcatc cgcatatttg gtcgctttga agaaccactg ctctaattct
    14521 tttttgatga cgggcgtgcc acaccgttcg caaacccgat catcaccttt gacctgttcg
    14581 cgcgccaggg ttgtattgtc cttcgggcac caatccaccg cggccttctt gcgatacgcc
    14641 agcccgtgtt tgtataactg gatgaagaac cattccgtcc agcgataata ttcagggtcg
    14701 gcagagaccg cttcccgttc ccaatcgaac atggcgccca tcgtgcgcat ctgcgtgcgc
    14761 atccgttcga tgttttgata cgtccacttc ttcgggtgga tgttgtgctt gatcgctgcg
    14821 ttttccgcat tcagaccaaa agcgtcgaac cccatgggga acaagacgtt gtatccctgc
    14881 atccgcttga accgagcccg cgcgtccgga ggcgtcatcg aaaaccaatg accgatatgc
    14941 aggtcaccgc tgggatacgg cagcatcgtc agcgcataat gcttgggttt ggtctcatcg
    15001 atcacagacc ggtagagatt atccgcttcc cattttgcct gccactttgg ctcgaaaacc
    15061 tgtggattaa aagccttatc tttcggcgcg ctttcgcttc cagctttcgt tgatttcgcg
    15121 ttgtttgtag atttttcttt catcgtcacc attcgttcct catttttata agtatcaggt
    15181 accagattcc gaatttcata aggaaattta tttgcaagta aaaaggcgcg gaagaagagg
    15241 cccctgtttc cagaagatgt aattaatccg aattttcata cgagcctcct ttcatatatc
    15301 ccctctcctt acgggagact gcgaagcgct ccgaaggaga ggttagggtg agggaaaaca
    15361 aaaaatcctt catccactac agggacgaag gacgcttggc gctccgcggt accacccagt
    15421 ttgtacggcg agaccatggt ctcgcaatac cactttaacg cgataacggg cttctccgtc
    15481 accagctact ctgcagcatg gctgcgttca ccagcgaagt ctctcgttcg agaaaacagg
    15541 cgagttcggt ctttattatc ccgtaaaggg gattagatcg gtattcccgt tggataccat
    15601 tgaagggctc tcacctcgct ttcccttctc gctgacggga tgacgtacta ctcctgccat
    15661 gacttgataa gtattttctc tattatagca caagagtgaa acgtcgagcc agtggctgac
    15721 ctgttgaact gcgaagcctc gcgcgcgttg aaaacactca ttttttgaat gtttaatgat
    15781 ataagttagc cactccctta gtctacttat gcgcacatcg actgtatcta ctactgcttc
    15841 gctcgtcgtt caattaatat aatattccac taccgagagc tctgagcatc atgcaatttc
    15901 aaaccaaccc atatctgatc tggcaattac ttcctggtat tgtgctgatc agcatcgggt
    15961 tatatattca aagccgccca gtcaagaagc gcgaatcgaa caacttctca ttgatgatgt
    16021 ttgccgggtc gctatgggcc tttgccaatg cgatccaatt gatcacgcct aacccaacct
    16081 ggcaaagatt ttggcatgcc gtcacctatg ttgcgattat gacggtgccg acttcatggt
    16141 ttctgttatc cacgaagctg acgaattttt tcccggaacg aatcgacaag atcgaaaaat
    16201 atctttggta tccgccggcg gttttttatc tctttctcat tacgagtggc tggcatggct
    16261 tatttttcac agccaccaat gtggtttcgg tgggtggcta tgttagcctg gaggatgagt
    16321 atgggatttt gttcttcctg cacacagcct attcatatgc cctgctggtc gcagggatta
    16381 ttattctcag tatttcgctc gtaacaaatt tcaaaaagta tggtttgcaa gcttacggtc
    16441 tgattatcgg tgttttggca ccgctggctg gcaatgccta ttttttgttc ggttcaccac
    16501 cggagggctt tccggatccc accccgatca ttttcactgt cactggtatt gcctttgcct
    16561 gggcaatctt tggcggtcat attctggaag tagtaccgct ggcgcatgaa gctatcgtgc
    16621 ggcaactctc cacaggtgtc atggtgcttg atggcgataa gaacattcgc gatatcaaca
    16681 atgcggcgct ggagatgctg gaactgccgt cgcgaactta tagcggcgct tctttgatgg
    16741 aactcgttca gcagaacatg gatgtggcgc tggtggtaaa cgatgcgctg gtttcatccg
    16801 ctcaggaaga taagaatatc caggtcgctt ttccaaaaac gcgtcgaacc ttcgacgtgc
    16861 atatttccca tcttggcgat aacctggata acacaaccgg atggttgatc cagttcacgg
    16921 acatcagcga gaaaagacag gcgcaggcag agctggcaac cgcccgtgag acgctggagt
    16981 ctgtgctgga tagtttgcag gatagttact tcgaagccga cgtcaacggt gtgatcacat
    17041 acgccaatca agcgctttgc aacagattga gactttccaa agctgaattg atcggaaaac
    17101 atttccggca tattacgatc cgcgagaaga tcagggaaat ttatgaaagt tttcaactgg
    17161 tctatgaaat gaaaaaatcg ctgggtcctt tcgaatatgc ctatcgttca aaagacgggc
    17221 gggtatatat tgccgagacc atcgtttcac ccatcatcga agacggtgtg gtagtcggca
    17281 cacgcggagt tttgcgcgat atcaccgacc gactgaaggt agagcaggag atcaaggagc
    17341 aaaaggatct attggacagt ctcttgcagc aatcaccaat cgccatggtg atcaacgaca
    17401 tgcagaagaa aatcacagtg gtaaacccgg catttgaaaa attgttcggc tattcgtcgg
    17461 aggaagcgat cggaaaaagc ctggataacc tgctctcgcc cacccgatca gaagacaacg
    17521 gcaatgaatt ttcgacgctg gtcatgaaga aaaaggaaac tcgtgaaaat cgccgcaggc
    17581 ggaaggacgg ctccctggtg gatgtggaga tcttcaccgc gccgttcttc gttggcggcg
    17641 aacgattcgg atatctggcc ttctataacg acatttcaga aagacttgaa gctgaagcga
    17701 atctggagaa aacccaaagc tcctattttg cagtgctcga aacattacag gatccctatt
    17761 tcgaagttaa tcgatccggt gtttatgtgt atgtgaacaa ggctttttgt gattcgatcg
    17821 gttataccag agaagaggtg ttgggaaaat tcttccgatt tgtttcctcc agacaatctg
    17881 tgcgatccac tattaaaaga tttgcggcga tgtttgaaaa cggtaaaccg atcccgccct
    17941 ttgattttat ttatcgccgt aaggatggtc aggaattccc tggtgagatt gtggtttcac
    18001 ccattatcga agatggcgaa gtatttggcg cgcgcgggat aattcgggat atttccctta
    18061 gagtggaggc agaggagatc ctgcggcagg ccaaggaagc cgctgaatcg cgagccggtg
    18121 agctggctgc catcaaccgc gtttcgacca cggtcagtca ctcgctggat ttgcgcgata
    18181 tcctgcagtc ggtctgctat gagttgacca atatctttcc cattcggaat gcaggcatcg
    18241 gactcctgac gcccgataaa aagaaactgg agatcgtcgc cttccatgcc agcgatccgc
    18301 aggaaaaaag cgtcaaaggg ttgtttctgc cagtcgaggg caacacttca tccatggaag
    18361 tgatccgcaa caaaaggacg atcgtgatcc aggatgccca aagcgacacg cgcacaagtt
    18421 ccatcgccga tctttcccgt cagcgcggca cgaaatcgat catgatcgtg ccgctcctgg
    18481 cgcgcggcga agccattggc acgatcggaa tgcctgccaa tgacccgaat tatgtgttct
    18541 ctaaaaatga gatcgagctg gcagagacga ttgccgggca gatcgccgcc gccgtggata
    18601 atgcccagtt acatgaaaaa accgagcacg cgctggacgt ggcagaacga gatctggaaa
    18661 tcggtcgcca gatccaatcc gggttctttc ccgagaaatt accccacatc ccgggctggg
    18721 agattgctgc gcacttcaat gccgcgcgcc aggtggctgg cgacttttac gatgcgttca
    18781 agatcaagaa ttcgaatttc agtgcgttcg tgatcgccga tgtgtgcgac aaaggtgtgg
    18841 gcgcagcatt attcatggtt ctgttccgca gtctgctgcg tgccttcagc gagacaacca
    18901 tcaccaggac cgatgtgcgt gaaaaactcc ttaatatcat tgtcagtacg aataatttca
    18961 ttgcggaata tcacggtaaa tccaacatgt ttgcgacact gttcttcggc gttctggatc
    19021 ctgagaccgg cacgctgtat tatgtgaacg gcggacatga accccctatc attctggatg
    19081 cacagggaaa tgtgacccaa cgattaatgc cgacaggtcc ggctgtgggt atgttcacag
    19141 agatggtttt ccgtgtggaa cagatcgact tcaacgaagg cgatatgttg atcggcttca
    19201 cggatggaac gactgacgca aaaaacgcat ctggaaaaca atttaccgag gatcgtctgt
    19261 tgcaaagcgt cagcaagccg tggacatcca acttcagcat gttgtatcag ctgaattcca
    19321 ggcttcagga acatattggc gcacagaacc aattcgacga tatcaccctg atttcgatcc
    19381 ggcgcaactc gctgtgcgat acgagtctgt ttcacacgat tgagcggccg gcacacctgg
    19441 ccattctcgg tgaactgcgt aattttgcag agcaggcggc ccaatacagc aatcttgtca
    19501 gcgatgatgt gtttgccttc aaactggcag tcgacgaact gtgtgcaaat atcatccagt
    19561 acggtttcga gggtcaggaa ccgggcagga tcgcgttgtc attcgaaaaa cagatggatg
    19621 tggcgcggct cctgataaag gacaatggga aatatttttc gcccgacgag gcgaaaagtc
    19681 cggatgtgca ggcggattgg gatgaacgcg agatcggcgg attgggcatc tacttcgtca
    19741 aggaattgat ggataatgtc acgtataata aagcagggga tggatataat caattcgttc
    19801 tggaaaaaag aattggaaaa caaagcagca actgaggaga tttgatcatg gagatcaagg
    19861 atagacaggt caacggagta acagtagtat cgctgatggg cagtatcgat gcaatgacag
    19921 ccccaaagat cacagaattc attcatgggc aaattgccaa cggaaacatc aagctggtgg
    19981 ttgaccttga cggtgtggac tataccagca gcgccggtct gcgggtcctc ctgggcgcca
    20041 tcaaggaaac ccgcgcgcag ggtggcgata tccgcctggc taaaattcaa ccggatgtgc
    20101 aaaaggtcct gaacttgagc ggtttcacca acatccttaa ggtgttcggc gaattggatg
    20161 ccgccgtttc gagttacgcc tgacccatcg cggacagatg tcgaggaaga tcgccctggt
    20221 gatcggctcg gggagtgtga aatgtgcggc agcactgggg ttattgcgtg tattgcaccg
    20281 tgaaaagatc gaagtggata tggtggtcgc cgggggcggt ggcagtattt ttggatcatt
    20341 gatcgccctg gggtacaacg tcgaagagat cgtagagaca aaccggttac tgtggacgcg
    20401 cgaagtcact gagcagccga accgtctggc gatgctgcaa atgctgctcc caaaattttt
    20461 cagaagtggc gaatacttcc atctgcgtga cgaccgggta atgaatgaac ggctgcgcgc
    20521 cgcatttggc gatacaacct ttgaaaaaac caggatccct ttgttcatca ccgccacaga
    20581 ctaccgatcc ggcaggcagg ctgtcatttc cgatggtcat atctatgcgg cggtgcgtgc
    20641 cagcattgcc ctgccattga tctttccgcc cagccagcgc ggcgaacaac tgctggcaga
    20701 tagttacctg tctgatccat tgccggttgg cgttgccatt cgcgagggag cggatatcat
    20761 tctggcaatg ggctttgaat cggtttccaa ggtccaaaga aattcctttt ccgagtatgt
    20821 attacatctt tcaggagtgg tctccaacaa tctgctgcag gcctcctatg ccttttacaa
    20881 cctcgcacat cattccgaag ttttattgat cgtgccgcag ttcaagtctg agattcacat
    20941 gttcgatacg cataaggtgc ccgagatcat caaagcgggc gagatggaag gtgaaaagat
    21001 catgccacaa cttaaacaca tgatggagat gcatggatga agacatcaaa accaaaaccc
    21061 cgcattgcat tggtgatcgg ttcgggcagt gtcaagtgcg cagccgcatt gggattaatg
    21121 aaggtgctgc aacgcgaaaa cattgctgtt gacctggtgg tgggatgttc tggcggggcg
    21181 atttacgctt ctctcatcgc cctcggttgg cctgtcgaac aagctacaga agccacgctc
    21241 agaatgtgga ccagcgaatt gaccgccaaa cgaaaccggc gggcgctgtt acaggttgcg
    21301 cttccgaaat ttttcaaatt cgacgagacc ttcggcttga tcgatgacag cctgatcaac
    21361 agcagattaa agcagggttt taaaaacgcc acatttgaag atacgaaaat tcccttgtat
    21421 gttgtggcga ccgaccttta caacggggag caggtggtca tatccgaggg ctccatcgca
    21481 gacgccgtcc gtggaagtgt atccatcccg tacatattcc cgccacacaa actgaatgga
    21541 cgctatctga tcgacggttt ccaaagcgac ccgctgcctg ttggcgtggc gatcaaggaa
    21601 ggcgcggata tcatcattgc gatgggcttc gaaagcccgt atcaggaaca gatcagttcc
    21661 ctgatgcggt acaacttcca ggtcagtagc gtcacatcga acaacctgct caagaccaat
    21721 tttgccttcc acaatctggc acatcactcc gagattatcc ctatcctgcc ggagttcgag
    21781 caccgcatca aacttttcga taccgacaaa attccgtatg tgatcgagca gggtgaaaaa
    21841 gcgtcggaaa aacaactgcg ctatatcaag gaattgcttg tcagataggc atgtgctgca
    21901 tgacgaatcg gcgtcgtctt ggtctggtga tcggttcggg tggattgaaa tgcgccgccg
    21961 ccatcggcgt tctgcaggta ttggagcagg agaacatacc gctcaatatg atcgttggct
    22021 gtagtggtgg agcagccttt ggcgcgtcga tcgccatggg actttcggca gaagcgatca
    22081 ttaaaacctt tacccaagcc tggacgcgca gttccacagg taaatttgat attcgttcca
    22141 tcctgaaaat cgcagttcca gagtgggttg cgtttgatga caagattggc attctggatg
    22201 atggcgggtt ggtacgcggc cttgttcacg cgtatggagc ggatgcctct tttgcagaga
    22261 cgcagatccc gtttcatgcg gtagcgaccg atcttcacac gggagaagcg gtggtcctat
    22321 ccgatgggat tcttgtcaag gcgctgcgcg ccagtgcagg catcccgatc ctgttcaagc
    22381 cagtggaatg gaatggcagg tggttgatcg acggcgggct gtcgaacccg ttgcctgtcg
    22441 atgttgcgat tcagcagggc gccgatgtca tcattgctgt cggttttgaa acgcctctgg
    22501 tgcaatctgt tgcgtcaccc gcaaattttg cattgcagat gttcaacatt ctgatcaatc
    22561 aattgctgtc ccgaaaattt gccttttaca acttggcgta tcactccgag atcattgcca
    22621 tcgttccgaa tttcgaccgc gagatccgtc tgaaggatgt gaacgaagtg ccccaaatca
    22681 tcgagcaggg acgcaaggaa accttgaagc atctcacgca cctgcaaagg atactgttcg
    22741 catagaatca taacgctcaa cgaggtgacc tgcatgaaaa acaacaagcg aatcgctctt
    22801 gtgatcggct ctggcgggat caaatgcgca gctgctgtgg gcttgtggcg tgtgctgcag
    22861 gaggaaaaca tcgaggtgga aagcctggtc ggttgtagcg gcggcagtct ctatgccgcg
    22921 ctgatcgcca atgatgcaga tttggttttg atgcaggaat tgtccagccg tttgtggacg
    22981 aaggaaatca tgcagggcta tac
//
LOCUS       DASTJP010000690         7860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_374831, whole genome shotgun sequence.
ACCESSION   DASTJP010000690 DASTJP010000000
VERSION     DASTJP010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374831"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFQ23_20195"
     CDS             complement(<1..371)
                     /locus_tag="VFQ23_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908982.1"
                     /translation="MYGKGILKGLSVTWKRFWDTYLEDISWVLRGKKRYYTEEGVKHR
                     ASKDQRGIFTVQFPEERLILPEEARYIPFLVYDEGPNGEKTQRCTSCGICAKVCPPQC
                     IWIVRSNDPQTGRPIPEPTEF"
     gene            complement(387..2129)
                     /locus_tag="VFQ23_20200"
     CDS             complement(387..2129)
                     /locus_tag="VFQ23_20200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET9908983.1"
                     /translation="MTTPALTSTIDLAARFPGVVTAETRAGFSGFLVEKDSLLEVATA
                     LRDEMGFDLLTAVTGVDYFPENKMEVVYHAYRTTGGPGVVFKVQVPRVDPVEVPSLIH
                     LWPGVDFQEREAWDLMGIKFIGHPDLRRILMWEGFEGYPLRKDWKEGFYEEDTKPFKS
                     RWPDGQFYMAEDKNPFKDNLNFPQNFDPEKWIPEGDALLYGALARYTVKNEDGLSTDR
                     IVINMGPHHPSTHGVFRAAMTLDGETIVGLKPVLGYLHRNHDKIGERNTYLQNMPYTD
                     RLDYFNSMTNNFGYAIAVEKLMKIPVAERAEYIRVIVAELTRVQNHLVFIGTLLNDLG
                     AMYTPSLYAFEERELILDIFEAISGARMMCNYFRFGGVVRDIPDDVMQKIKDLVFERL
                     PAKTDEMDRFLTENEVLVSRMKGVKMIDAEDAVRYSITGPVLRAAGVPYDIRRADPYG
                     IYERFDFDVAVRHNGDLMDNYLIRIDEIRQSLRILEQALKQIPAGPINSQKPQYQVRV
                     PAGEAYGRIESPKGELGFYLVSNGKPNPWRYHVRPASFVNLTALEKICIGNKIADFVA
                     FLAMFDIVMGECDR"
     gene            complement(2132..2803)
                     /gene="nuoB"
                     /locus_tag="VFQ23_20205"
     CDS             complement(2132..2803)
                     /gene="nuoB"
                     /locus_tag="VFQ23_20205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET9908984.1"
                     /translation="MENEKLSLPEYDIPADLEHAIAVTTLDRLYNWGRRSSVWPLMFG
                     LACCAIEMIAAQTARYDMARFGMEVMRPTPRQADLLLVSGTVTKKMVPAIVRLYNQMP
                     EPKYVVAMGACASGGGPFKEGYNVVAGIDKFLPVDVYIPGCPPTPQALIAGLIKLQEK
                     IDKQSIKRALWYNKKKQDSNYVPVPILGPDLIDPRRNTEIKITAARSNEMQPSEHDKH
                     APREG"
     gene            complement(2793..3002)
                     /gene="iscX"
                     /locus_tag="VFQ23_20210"
     CDS             complement(2793..3002)
                     /gene="iscX"
                     /locus_tag="VFQ23_20210"
                     /inference="COORDINATES: protein motif:HMM:NF016284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HET9908985.1"
                     /translation="MTLSWESTYAVALELRRQHPDVNIEDVSLQQVYQWTLQLSEFED
                     DPALANDDILYAIYQDWFEENIHGK"
     gene            complement(2999..3313)
                     /locus_tag="VFQ23_20215"
     CDS             complement(2999..3313)
                     /locus_tag="VFQ23_20215"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HET9908986.1"
                     /translation="MKRNLYLVCTILGLIIPWSILISRFNSQEPIAGIFGFLFVNAYS
                     ASFTADLVFSIMVFWIFVILEAQRLKMKNSWLYILASLTVGLSFSLPLFLFFRERQLE
                     DK"
     gene            complement(3313..4785)
                     /locus_tag="VFQ23_20220"
     CDS             complement(3313..4785)
                     /locus_tag="VFQ23_20220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9908987.1"
                     /translation="MEFQNYINGKWVKGRNTFQTINPANEELVAEIAQAELADVDAAV
                     NAAKDAFRSWRLLPAPLRGEMLFKVGDVLKQKKEELSQLLTRDMGKVIAEARGDVQEA
                     IDMAYYMGGEGRRLLGYTAPVEMPNKFGMAVRDPAGVVGLITPWNFPIAVPSWKLYPA
                     LVAGNTIVWKPSPETPAISAAFVQVFEEAGLPPGVFNLIMAPGAEVGKALVEHPDVRV
                     LSFTGSTTTGRAIAQSAAKLNKKLSLEMGGKNAIIVMEDANVELVTDATLWASFGTSG
                     QRCTAASRLIAHKGIVGQVKESLVERTKKLRLGDGLDPKIDVGPVINKAALDRIHNYV
                     QIGQKEGARALVGASVADINGKGFFYSPTLFDGVSPGSTLEAEEIFGPVLSIIEVNSL
                     EEAIEVNNRSQYGLSTSIFTQDVNRAFTAMRDIATGLVYINHGTTGAEIQFPFGGVRG
                     TGNGHREAGQAALEVFTDWKSIYVDYSGKLQRAQIDNREE"
     gene            4930..5517
                     /locus_tag="VFQ23_20225"
     CDS             4930..5517
                     /locus_tag="VFQ23_20225"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HET9908988.1"
                     /translation="MKTVRQKVLSYLNKTHTASAREISRVLKMSVATVRHHLRVLVSD
                     GRLIESAVLPHEKKGRPEKVYSLPQSALGDNLFRLSDALLEETGPAVAMDRIAKRIAG
                     DSTFVNEPLAKRLNRTVEKLNEMNYHAHWEAAAEGPRLILGHCPYASIIEKHPELCVM
                     DSALLEELTGNAAKQLTKIGHDGKLSCGFALTNKS"
     gene            complement(5528..6943)
                     /locus_tag="VFQ23_20230"
     CDS             complement(5528..6943)
                     /locus_tag="VFQ23_20230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9908989.1"
                     /translation="MTYSNTPRKEAIRSVTDAIHFPFWLDDPARPEPEPVLTKDVATD
                     LLVIGAGFTGLWTALLAKEENPSREIVILEAGEVAQGATGRNGGFMDSSITHGFYNGL
                     SRWPRELSTLLALGITNLNEIEATITRLGINCDYRRTGDIDMATQPHQVDEIKEELEI
                     AAPYQTNFQFLDCDEVQAVVKSPIFIAGLKRPDSSLVNPAHLAWGLRKACLESGVKLY
                     ENSPVTKLENNIKNVLARTAQGSVRAARVALATNAFPPLLKRISFYIVPVYDYALMTE
                     PLTPIQRESIGWYGRQGISDASRQFHYTRTTADGRILWGGYDAVYYWNNGFGPHLENS
                     PIMFARLAEHFFQTFPQLEGIRFSHAWGGAIDTCSRFSPFWGTAHHGKTAYAMGYTGV
                     GVGATRFGARVMLDILDQKKTERTDLEFVRTKPLPFPPEPLRSLGINLTRWSMDQADR
                     NQGRENLWLRAMNWLGLGFDS"
     gene            7082..>7860
                     /locus_tag="VFQ23_20235"
     CDS             7082..>7860
                     /locus_tag="VFQ23_20235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9908990.1"
                     /translation="MNLFQRYLAEEFAEDYEEGRLSRRDALKMITSVTGSLIVANSIL
                     AACAPPPEESGVALTPTDGPAVASTIQPAATNDVSTSAPDPTYGTVMPDDPAVIASEI
                     QFPASDGASLIGYLAQPSSGELAPVILVCHENRGLTPHIQDVTRRFAKAGYVGLAVDL
                     LSRQGGSTVVGEGNVPGALGNILPDQFVEDFKSGWQYLANLSFADTARVGMTGFCFGG
                     GVTWRVATQMPELLAAVPFYGPHPPVEDVPNINAAVLAIYG"
BASE COUNT         1959 a         2023 c         1920 g         1958 t
ORIGIN      
        1 aaaaattcag tcggttcggg tatgggacgc ccggtctgcg ggtcattcga acggacaatc
       61 cagatgcatt gtggagggca aacctttgcg cagattccac aggatgtaca gcgttgggtc
      121 ttttcgccat tcgggccttc atcgtacacc agaaacggaa tatagcgcgc ctcctcaggc
      181 aggatcaacc gctcttccgg aaattgaacg gtgaagatgc cgcgctgatc tttactggcg
      241 cggtgcttta cgccttcttc cgtgtaataa cgtttcttgc cgcgcagcac ccacgagata
      301 tcttccagat aggtatccca aaaccgcttc caggtaacgc ttagtccttt gagaattcct
      361 tttccataca taaataaatc ctccgattaa cggtcgcact cgcccattac gatatcgaac
      421 atcgctagaa aagcaacgaa gtctgcaatc ttgttgccaa tgcagatttt ttcaagtgcg
      481 gtgaggttga caaacgacgc cggtcgtaca tgatagcgcc atggattcgg cttcccgttc
      541 gaaacaagat agaaacccaa ctcgcccttg ggagattcaa tccgaccata cgcctcccct
      601 gcgggaacac gaacctgata ctgtggtttc tgcgaattga tcggtcccgc gggtatctgc
      661 ttaagggctt gttccagaat ccgcagcgac tgtcgtatct cgtcaatgcg gatcaggtag
      721 ttatccatca ggtcaccgtt gtggcggacc gcgacatcga aatcgaatcg ctcataaatc
      781 ccgtaagggt ccgcgcgccg aatatcatat ggcacacccg cggcgcgcag cacaggacct
      841 gtgatcgaat atctgactgc atcctcggca tcgatcatct tgacaccctt catgcgcgag
      901 accagcacct cattctcagt aagaaaacgg tccatttcat cggttttcgc gggtagtcgc
      961 tcaaagacga gatcctttat cttttgcatt acatcatcgg gaatatcgcg aactacacca
     1021 ccaaaacgga aatagttaca catcatacgc gcaccagaga tcgcttcgaa gatatccaga
     1081 atgagttcgc gctcttcaaa tgcatacagg gacggtgtgt acatcgcgcc gagatcgttc
     1141 aacaaggtgc caataaagac caggtggttt tgtacccgcg ttaactcggc tacgatcact
     1201 cggatatact ccgcacgttc cgcaacgggg atcttcatca acttttcaac agcaatggcg
     1261 tagccaaaat tgttggtcat cgaattgaag taatcaagtc gatcggtgta cggcatattt
     1321 tgcagatacg tgtttcgttc gccgatcttg tcgtgatttc gatggagata tccgagcacg
     1381 ggtttcaagc ccacaatcgt ttcgccgtcg agggtcattg ccgcgcggaa gacgccatgg
     1441 gtggatggat gatgcggacc catattaatg acaattcggt ccgtacttaa tccatcttca
     1501 ttcttcactg tgtatcgcgc cagcgcgccg tagagtaaag catcaccttc gggaatccat
     1561 ttttcggggt cgaagttttg cggaaagttc aaattatcct tgaacggatt cttatcctct
     1621 gccatgtaaa actgaccgtc gggccagcga cttttgaaag gtttcgtgtc ttcctcgtag
     1681 aatccctcct tccagtcttt gcgaagtggg taaccttcaa acccctccca catgaggata
     1741 cggcgcaggt cagggtgacc tatgaacttg atccccatca gatcccaggc ctcgcgttcc
     1801 tgaaaatcaa ctcccggcca gagatggatc aacgacggaa cttcaaccgg atccacacga
     1861 ggcacctgaa ctttaaatac cacaccgggt ccgcccgtcg tgcgatacgc atggtagact
     1921 acttccattt tattttccgg aaaatagtcc acacctgtca cggcggtaag taaatcaaac
     1981 cccatctcat cgcgcagggc cgtagcgacc tcaagaagcg aatctttttc tacgaggaaa
     2041 ccactaaagc cagcccgcgt ttcggctgtg acaacgccgg ggaatcgggc tgccaggtca
     2101 atggtcgaag taagagctgg agttgtcatc ctcatccctc tcgtggtgcg tgcttatcat
     2161 gttcggatgg ttgcatctca ttgcttcgcg cagctgtgat cttgatctcg gtattccgcc
     2221 gcgggtcaat caaatcaggt ccaagaatag gaacaggcac atagttcgag tcctgctttt
     2281 tcttgttgta ccagagcgcc cgcttgatgc tctgtttgtc tattttttcc tgaagcttga
     2341 tcagtcctgc aatgagtgcc tgtggggtgg gagggcatcc ggggatgtat acgtcgaccg
     2401 gtaaaaattt atcaatacct gccaccacgt tatagccctc cttgaaagga ccgccacctg
     2461 acgcgcatgc gcccatcgcg accacatatt ttggttcggg catctggtta tagagcctca
     2521 cgatggcagg taccattttc ttggtgactg tgccggagac aagcaacaaa tctgcctgac
     2581 gcggggtagg gcgcatcact tccatgccga agcgcgccat gtcataacgc gccgtctgtg
     2641 ccgcgatcat ttcaatcgcg caacaagcca gcccgaacat cagcggccag acggatgacc
     2701 ggcggcccca gttatacagc cgatccagag tcgtgacggc tatggcatgt tctaaatcag
     2761 ctgggatgtc atattccggt aaactgagtt tttcattttc catgaatgtt ctcctcgaac
     2821 cagtcctgat aaatcgcgta caggatatca tcgttggcaa gcgccgggtc atcctcaaac
     2881 tctgaaagtt gcaacgtcca ctgataaact tgttgcaggc ttacgtcttc gatatttaca
     2941 tcaggatgtt gcctgcgtaa ttccaaggcg accgcatatg tagattccca gctgagcgtc
     3001 atttgtcttc caactggcgt tctctgaaaa ataaaaacaa gggcaacgaa aacgacagac
     3061 ccactgttag agacgccaaa atataaagcc acgaattttt catcttcagc cgttgcgcct
     3121 ccagtatcac gaaaatccaa aagaccatga tggagaaaac cagatcggcg gtaaacgacg
     3181 ctgaatatgc attgacaaac aggaaaccaa agattcccgc aattggctcc tgtgaattaa
     3241 agcgcgagat cagtatgctc caaggaatga tcaaacccag tattgtgcag accaaataca
     3301 aattcctttt catcactctt ctcgattgtc aatctgcgcg cgctgcagtt tgccggaata
     3361 atccacgtag atcgacttcc agtctgtgaa cacttcaagc gcagcctgac cggcctcacg
     3421 atgtccgttg cctgtgccac gcacaccgcc gaacgggaat tgaatctctg cgccggtagt
     3481 tccgtggtta atgtaaacaa gtccggtggc aatatcgcgc atggcagtga aggctcggtt
     3541 cacgtcctga gtgaagatcg aggtcgagag tccatattgt gagcgattgt tgacttcgat
     3601 ggcttcttcg agagagttaa cttcaatgat cgacaatacc ggaccaaaga tctcttccgc
     3661 ctccagcgtg gacccggggc tgacgccgtc aaacagggta gggctgtaga aaaagccttt
     3721 tccgttgata tccgccacag acgcacctac aagtgctcgg gcaccttcct tctgaccgat
     3781 ctgcacatag ttatggattc tgtctaaggc agctttgttg atgacaggac caacatcgat
     3841 ttttggatca agaccgtctc cgagtcttaa tttcttggtt cgctctacca gcgactcttt
     3901 cacctggccg acaatccctt tgtgtgcgat caatctgctt gcggctgtgc aacgttgtcc
     3961 gctagtgccg aaggaagccc acagagtcgc gtctgtgaca agttctacgt tagcatcctc
     4021 catcacaatg atggcattct ttccgcccat ttcaagcgat aacttcttgt ttagctttgc
     4081 agcagactga gcaatggccc ggcctgtcgt tgtggaacct gtaaatgaga gcacgcgcac
     4141 atcaggatgc tcaaccaatg ccttgcccac ctccgctccc ggagccatga tgaggttgaa
     4201 caccccaggc ggtaagcctg cctcttcaaa tacctgcaca aaggcagcag agatcgcggg
     4261 agtttcgggt gatggcttcc agacaatcgt gttacctgca accagcgcag ggtataactt
     4321 ccaggaggga acggcaattg gaaaattcca cggagtgatt aatcccacaa ccccggccgg
     4381 gtcgcgaacg gccatcccaa atttattcgg catttcaact ggcgcggtat accctaagag
     4441 gcggcggcct tcacctccca tgtaataggc catgtcgata gcttcctgca cgtctccgcg
     4501 cgcttcggca atgactttac ccatatcacg agtcagcagt tgtgaaagct cttctttttt
     4561 ctgtttaagt acatcgccca ctttgaaaag catctctccg cgcaacggag cggggaggag
     4621 tcgccaggat ctgaaggcgt cttttgctgc gttgacagcc gcgtcgacat cagctaattc
     4681 tgcttgcgca atttctgcca ccaattcttc gttggctggg ttgatcgtct gaaacgtatt
     4741 tctgcctttg acccacttac cattgatgta attttgaaat tccatcgttt cgccttccat
     4801 ggtgagaaga ataaatttgt tgtgcgaaaa gccacattcg aaataaatta tagcatctaa
     4861 aaaacgaaag tcaaatttat ttatataaat atatatttac gaaaataata aaatacatat
     4921 aatggatgta tgaaaaccgt acgacaaaaa gtactgtcct atttgaataa gacacatacg
     4981 gcctctgctc gggaaatttc tcgagtcttg aaaatgtccg tcgcaactgt aagacatcat
     5041 cttagagtgt tggtttctga tggacggctg atcgagtcgg cggttttgcc ccatgagaag
     5101 aagggcaggc cagaaaaagt atattcatta ccacagtcag ctttgggaga taacctgttc
     5161 cgcttaagtg acgctctttt agaggagaca ggtccagctg tggcgatgga cagaatagct
     5221 aaacgtattg ccggcgactc tacttttgtg aatgagccac ttgcaaagag acttaatagg
     5281 accgttgaga aactaaatga aatgaattat cacgcccact gggaagccgc tgccgaagga
     5341 ccacgcttga tcctgggaca ctgtccatat gcaagcatca ttgagaaaca cccggaattg
     5401 tgtgtaatgg attcggctct gcttgaagag ttaacaggca atgccgccaa acagttgacg
     5461 aagatcggcc atgatggaaa attatcgtgt ggctttgcat taacaaacaa atcctgacgc
     5521 agtcaaatta actatcaaag cccaatccaa gccagttcat tgcgcgcagc caaagatttt
     5581 cgcgcccttg atttcgatcg gcctgatcca tggaccagcg tgtgagattg atgcccagtg
     5641 aacgcagggg ttcaggagga aatggtaagg gtttggttcg aacgaattcc agatctgtac
     5701 gctcggtctt tttctgatca agaatatcaa gcatgacgcg tgcaccgaac cgggtggctc
     5761 ctacacccac tcccgtatag cccattgcat aggcagtttt tccatggtgt gcagtacccc
     5821 aaaacggact aaagcgtgaa cacgtatcta tcgctcctcc ccaggcatgc gagaagcgaa
     5881 taccttccaa ctgaggaaaa gtctggaaga aatgttccgc cagtcgcgcg aacatgatcg
     5941 gagaattttc aagatgaggt ccgaagccat tgttccagta atagactgca tcgtacccgc
     6001 cccatagaat gcgtccatct gcggtggttc gcgtgtaatg aaattgacgg ctcgcatcgc
     6061 taatgccctg gcgtccatac catccaatcg actctctctg gatgggggtc agcggttcag
     6121 tcatgagggc ataatcataa acagggacaa tgtaaaacga aatgcgcttg aggagaggcg
     6181 gaaaggcgtt cgttgccagg gcgacgcgtg cagcgcgtac gcttccttgc gccgttcgag
     6241 ccagcacatt cttaatgtta ttctccagct ttgttaccgg gctattttca tataacttca
     6301 caccagactc aaggcaggct tttcgcaatc cccatgcgag atgggcaggg ttgactagag
     6361 atgagtctgg ccgtttcaac cctgcaatga aaataggcga tttaacaacg gcctgtacct
     6421 catcacaatc caaaaattgg aagttggttt gatacggagc ggcaatttcc aattcctctt
     6481 tgatttcatc cacctgatga ggctgtgttg ccatatcgat atcaccggtg cgcctgtaat
     6541 cacaattaat accaaggcgc gtaatggttg cttctatctc attaagattt gtaatcccaa
     6601 gcgccagcaa tgttgacaat tctctgggcc agcgggaaag cccattatag aatccatgtg
     6661 taatggatga atccataaaa ccaccgtttc gcccggtcgc tccctgggcg acctcacccg
     6721 cttcaaggat gacgatttcg cgcgaaggat tctcctcttt tgcgagcagt gctgtccata
     6781 gacctgtgaa tccggcgccg attacaagca agtcagtagc aacgtctttg gttaaaacgg
     6841 gctccggttc tggccgggca gggtcgtcga gccaaaatgg gaagtggatt gcatcggtaa
     6901 ctgatcttat ggcttctttt cgcggtgtgt tggagtacgt catttcaata ttttacctaa
     6961 tgagactcag attaatcatc tgcaggcagg cttgacgaaa ttcatctcaa tggctaaatt
     7021 tgtagtgagc aaccggaggg aatgaaatgt tttgtaaaaa ttcggatttg aggagatatc
     7081 gatgaatctt tttcaacgct acctggcaga agaattcgca gaagattatg aagagggaag
     7141 gctttcacgt cgtgacgcat tgaagatgat cacgtctgtc accgggagtt tgatcgtcgc
     7201 caattcgatc ctggcggcct gtgcacctcc acctgaagaa tcgggtgttg cgctcacgcc
     7261 aaccgacgga cctgcagtcg catcgaccat tcagcccgct gccacaaatg acgtgtctac
     7321 atccgcgccg gacccaacgt acgggacagt gatgcccgac gacccagctg tgatcgcgag
     7381 cgaaattcaa tttccagcgt ctgatggtgc ttccttaatc ggatatctgg ctcaaccatc
     7441 aagtggtgag ctggcaccgg tcatattggt ctgccacgaa aaccgcggac tcactcccca
     7501 cattcaggat gtgacgcgac gattcgcgaa agccgggtat gttgggctgg cggtggacct
     7561 cttgtcgcgt cagggaggca gcactgttgt tggcgaagga aacgtacctg gtgcgctggg
     7621 taacatctta ccggatcaat tcgttgaaga ttttaagagt ggatggcaat atctggcaaa
     7681 tttatccttt gcagatactg cgagggtcgg catgaccggc ttttgctttg gaggaggtgt
     7741 tacctggcgg gttgccaccc aaatgccaga gttattggcg gccgttcctt tttatggtcc
     7801 tcatccgccg gtggaagacg ttccgaatat taacgctgcc gttctggcga tctacggaga
//
LOCUS       DASTJP010000691         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_480532, whole genome shotgun sequence.
ACCESSION   DASTJP010000691 DASTJP010000000
VERSION     DASTJP010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480532"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFQ23_20240"
     CDS             complement(<1..306)
                     /locus_tag="VFQ23_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908991.1"
                     /translation="MLLNKPGLRHVLFVFLVVVISLGAVGSAAAAQFPDVIPLPNGFR
                     PEGIAVGSGSTFYVGSIPTGAVYRGDLRTGEGDVLVPAQSGRAAIGLKYDDRTGLLFV
                     "
     gene            complement(493..795)
                     /locus_tag="VFQ23_20245"
     CDS             complement(493..795)
                     /locus_tag="VFQ23_20245"
                     /inference="COORDINATES: protein motif:HMM:NF042532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protealysin inhibitor emfourin"
                     /protein_id="HET9908992.1"
                     /translation="MTTITFDRTGGVIGNDLHLDLNLDDLSEDEAQHLIRLIDEADFY
                     NVPEHTADQFTSDEYKYTISVNSERASHTVHTSDHSMPKHLLPLIKELTMLKILHH"
     gene            1088..1651
                     /locus_tag="VFQ23_20250"
     CDS             1088..1651
                     /locus_tag="VFQ23_20250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9908993.1"
                     /translation="MKKFTVDDIDLVRQIAQEQNGALHELFDRYNRLVFSVAFAIVGD
                     RSTAEEVTLDVFVQVWKHAATYRADRAKVSTWLIAITRHHAIDILRWQKSRPEADSLN
                     WDGISLQNGPTSHNLEEHVEIAMQRERVQKAMSQLPEDQREPLVMAYFKGFTQSQISE
                     ALGHPLGTIKTRIRLGMQKLRKLLEGD"
     gene            1722..2435
                     /locus_tag="VFQ23_20255"
     CDS             1722..2435
                     /locus_tag="VFQ23_20255"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET9908994.1"
                     /translation="MTGNSHILESLPAYVLGSLDESEARLVAEHLAGCHLCRTELHAF
                     QDTADHLALAAPAALPSEDLKHRFAERMEAIKSSRQHRENIRSPRRLVPIGGLIAALL
                     VFALLTSNLILWQRFNNLEVLAGPLGMRAITLQNTEAAPAASGFVIISSDGLEGVLVV
                     DELPMLDANHEYQLWLEDGDTTSGAVFLVDESGYRGLRIEAQQSLLLYSSVRITVEPA
                     GGSALPTGETVLDGSLFNP"
     gene            complement(2514..>2854)
                     /locus_tag="VFQ23_20260"
     CDS             complement(2514..>2854)
                     /locus_tag="VFQ23_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9908995.1"
                     /translation="FALTIGVTRFIEKPVAMDKFVPIIEKILKQGEHIPAEPINEVDF
                     YDGYRKRLEIKLNQKNIQIARDERLLETLSDAEKPSFQTSLLQAINEREEIKNLLDQI
                     REQREKYSSS"
BASE COUNT          718 a          677 c          715 g          744 t
ORIGIN      
        1 tacgaataac aggcccgtac ggtcatcgta cttgagcccg atcgccgcac gtccgctctg
       61 tgccggaaca agaacgtctc cctcgcccgt ccttagatcg cctctataga ccgcgccagt
      121 cggaatcgaa cccacataaa aagtagaacc acttccaacc gcaatgcctt ccggccgaaa
      181 gccgttcggc agggggataa cgtctgggaa ctgtgcagcg gctgcgcttc ctacagcgcc
      241 aagactgatg acaaccacaa ggaaaacgaa tagcacgtgg cgcaagccag gtttattaag
      301 taacatttct aactccttat ttcgaaatta tgtagattag agaacaaata tggaacaaga
      361 ccatcagcga atgagaatgc tcgccgccac gtcctgccgt ttaatctctg caaatttata
      421 cgtggattgc tatgatatcg gatgcgtgaa tgataggatt ctcacaaaag tcaatcgaac
      481 aatccactcc cgctagtgat gcaaaatctt caacatcgtc aattccttga tgagtgggag
      541 cagatgcttt ggcatactgt ggtcactggt atgcacggta tgacttgccc gctcactatt
      601 cactgagatc gtgtacttat actcatccga cgtgaattga tctgcagtgt gttcaggcac
      661 attgtaaaaa tctgcttcgt cgatcaatct gatcagatgc tgtgcttcat cttccgacaa
      721 atcatccaga ttcaggtcga ggtgcaggtc attgccgata acaccgccag tccggtcgaa
      781 ggtaattgtg gtcatatctt cttagccttt ctgtgattta agtaggaacg cgaaatgccg
      841 caaacggacg aatcggagtc caatgcaggt tcgaggtatg cagctcgttg atattcgtcc
      901 gcaggggata cggcactcaa aaccgtgtac gagtatatgt aatgggattt gttttttcaa
      961 cccttaccct ggtttactta ataattaaaa tttagtaaag ttgtgtctga caatttccag
     1021 gcggagaata ttggtgctgg ctcttacctt gacaatcccg ccaggataac atacgatttt
     1081 tttcttcatg aagaaattta cagtggatga catagacctt gttcggcaga tcgcgcagga
     1141 acaaaatggc gcgcttcacg agctttttga ccgatataac cgtcttgttt tcagtgtggc
     1201 ttttgcaatc gttggcgatc gcagcacggc tgaggaagtt acgctggatg tattcgtgca
     1261 agtttggaaa catgccgcaa catatcgagc ggatcgggca aaggttagca cctggctcat
     1321 tgccatcacg cgccaccacg cgattgatat cttgcgttgg caaaaatccc gtccagaggc
     1381 ggatagcttg aattgggatg gcatctcgct acagaatggg ccaacctcgc ataacctgga
     1441 ggaacacgtg gaaatagcga tgcagcgcga acgtgttcag aaagccatgt cgcaattacc
     1501 agaagatcag cgcgaacctc tagtgatggc ctatttcaag gggtttactc agtcccagat
     1561 ttcagaggcg ctggggcacc ctctgggcac cattaagaca cgcattcgac tgggcatgca
     1621 aaaacttaga aaattgttgg aaggggatta gggtatgatt aataaatccg tatgggctca
     1681 ctttgcgtat tttattagtg agaaataggt aaggctcttc aatgactggc aattcacaca
     1741 ttctggaatc gttacctgcc tatgtcctgg gcagcctgga tgagtccgaa gcgcggctgg
     1801 tggcagagca cctggctggc tgtcacctgt gccggacaga acttcacgcg tttcaggata
     1861 ctgccgatca tctggcgttg gctgcccccg cggcgctgcc ttctgaagat ctaaagcata
     1921 ggttcgcaga acgcatggaa gcaatcaagt cttcacgcca gcaccgtgaa aacatccgca
     1981 gcccaaggcg acttgtgccc attggagggt tgatcgcggc actcctggtt ttcgctctcc
     2041 tgacctcaaa cctgattcta tggcagaggt ttaataacct ggaagtgctg gcgggacctt
     2101 tggggatgcg cgcgattacc ctccagaata cggaggctgc tcccgctgca agtggatttg
     2161 tgattatcag ttcagatgga ttagagggcg tgcttgtcgt ggacgaattg ccaatgctcg
     2221 atgccaacca tgaatatcaa ttatggttgg aagatggaga tacgacgagt ggcgcggtct
     2281 tcctggtaga tgaatctggc tatcgagggc tccggattga agcacaacaa tcattactac
     2341 tttattcatc cgtccgcatc acggttgaac cagctggggg cagcgcctta ccaaccggcg
     2401 aaactgtttt ggatgggtca ttattcaacc cgtaggacaa tgcaatttaa ctaaaaacga
     2461 actgcctgac cattcgtcag ggcgttcatt tttttaagct tgagtggttt tcattatgag
     2521 cttgaatatt tttcacgctg ttcgcggatc tggtcaagaa ggtttttgat ctcttcacgt
     2581 tcgtttatgg cctgaagcag ggaggtttga aaggaaggtt tctccgcatc agaaagagtt
     2641 tccagcaggc gctcatcgcg ggcaatttgt atgttcttct gattgagctt aatttccagg
     2701 cgtttgcgat atccgtcgta aaaatcaact tcattgattg gctcggctgg aatatgctca
     2761 ccctgcttta ggatcttttc aatgataggc acaaacttgt ccatcgcgac gggcttttcg
     2821 ataaaacgcg tcaccccgat ggtcagcgca aatg
//
LOCUS       DASTJP010000692        12118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_903389, whole genome shotgun sequence.
ACCESSION   DASTJP010000692 DASTJP010000000
VERSION     DASTJP010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903389"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..4470)
                     /locus_tag="VFQ23_20265"
     CDS             complement(370..4470)
                     /locus_tag="VFQ23_20265"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9908996.1"
                     /translation="MLSKNRKPFILQFALILALMGGAFGVQTVQAVTHTVININDSGP
                     GSLRQAIANAAPGDVISFDSSLAGQTIMLASQMNINKNLIIDGVGLNPRVEISGGNAV
                     RIFLIDAPSTIAPTIRNVVLKEGKQTGSSYTNFGGAMFVGNNTTLTLENVRITNNTAY
                     SAGAIYISPYAIVNISNSEIIGNRSDVEAGAILVKSIGILNLRGNVISNNVAGGAGTI
                     YFSGATNTSLIENNLFENNSAAAGGAIVGELGNARIEVRNNLFAGNHATTARAGAVYF
                     SASTIPTLIILENNTFYDNDAVGEGGGAYLGAAVDYYLVNNTFSNNAATMGGNLYLGP
                     GASVPKMYNNLFANHAGGGDCYAFYNSFINGGNNLVEDGSPECKPTLTGDPMLGPLAD
                     NGGSTQTMALLPGSPALNAGNTTHCPAADQRGIARPQGSGCDIGAFEYVPLHLSLTGN
                     QTLGGVASADEDILKFDGTNWSLFFDGSDVGVAGSDLFGFSIVDADTILMAFNTSFTL
                     NGMSVTPRDVVRFEATSLGSNTAGAFSMYLNGIDVGLDVSAENIDSVNLLLDGRVLIS
                     TTGNPVVTGVTGGRDEDVLAFVPTSLGNNTSGTWSMYFDGSDVGLAETSGEDVDALDV
                     LNGKVYLSTVNDFSVNGVTGADEDVFVCAPTSLGNVTACNYSSVLYFDGSAWGLAAND
                     VDGMSIFASGFTSPPTGTPTPTASATPSQTRTSTATSTPTNTATLGASTPTHTATATP
                     TVTPTQTATPTPIVTQTPGSSDLIFADGFESGNLSAWTASAINGGDLSVSSSAALVGA
                     QGLQAVINDTNTIQVTDDSPNAEPRYRVRFYFDPNSISMASGDAHFIFKGFMGTSTEV
                     LRVEFRQSAGLYQIRTGAFQNDSSWVYSDWYTISDGSHSIELDWSAETTSGPTNGSLT
                     LWIDGIGKVTLAGVDNSGKRIDRIRLGALTGIDAGTRGTYYFDAFESRRQNYIGPVPA
                     STTPVQVYVGGILQNSYNVPAQSSERASYTASGMMRVLSTNGGNILASQRFVHNHNGS
                     LWTGYSEQMGFPENQLATSYAFPWYDNVNHDSRLRVANPGNSHAAVTVSIGGIVQGTH
                     SVPPGGSLLVSYAGLNNGPVKVESTNGISILASMQVADNTGSAWTDVMGLPLGQVRTS
                     YVLPWYDNVNLNAQLRLGNIGNFSTTVTVAVGGVVQGNYVLAPNQAIQVAYNALNGGP
                     VRVESSASVPIVASIRFAHFNGSTWTNYSETMGHRSGSLDTHYYFPVYDNVNHNSQLR
                     LVNVGTGSTTVNVTINGVLQGSYLLQPNQGQQVSFAGIDSGPVLVQSMNGQPIIASEI
                     VSYFDVSAGTSYSEMIGLPFAHLTTSYLFPAYDNSNIDSQLRFGVP"
     gene            complement(4679..5962)
                     /gene="odhB"
                     /locus_tag="VFQ23_20270"
     CDS             complement(4679..5962)
                     /gene="odhB"
                     /locus_tag="VFQ23_20270"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF004309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET9908997.1"
                     /translation="MAIKILVPELGESVLDATVSRWLKKQGDFVNVGEVLVELETDKV
                     NLEVGAKGSGVLQKIDVPEGKDVKVGDVLGTIEEKAEEPKGSSAPTPKATEEIEVEKE
                     EETVPVASNNDKGEAQRQTPPPVTPVASRLARANDVDLSKVSGTGSEGRVTKADVELF
                     LQGQEKEKDSDNQVPAEAEREAQPVVSATTTPSSERGEERTRMSRRRRTIAQRLLEAS
                     QSTAMLTTFNEIDMGAVMDIRSRRNEEFQKRFGIKLGFTSFFVKASVSALKAFPQINA
                     EIDGEDIVFKHYYDIGTAIGSSEGLVVPVIRDADKLSFAEIEKQVKDFVAKTDQGKLS
                     IEDLRGGTFTITNGGVFGSLMSTPILNPPQVGILGLHKIEERPIAQNGQVVIRPMMYV
                     ALSYDHRIVDGREAVQFLVHIKQLLEDPAWMLVEG"
     gene            complement(5967..8765)
                     /locus_tag="VFQ23_20275"
     CDS             complement(5967..8765)
                     /locus_tag="VFQ23_20275"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET9908998.1"
                     /translation="MALEKDFHGPNFGYILELHQRYLDDPASVDEATRKLFASWSPNG
                     AGPALAAPRSLLSVTGAVNLAWSIRSYGYLSARLDPLNNTPPIAEDPLLTLTFHKLQE
                     EDLFTLPAEILKLPNQHGDENAQQAIDSLRAIYCGTIGYDYGHIRIPEERNWLYQTAE
                     TGHYRPPQQNVDEKQLLERLTQIESFELFLHRIYPGKTRFSIEGLDMLIPMLDEIIAS
                     ASDEKIRAVLIGMAHRGRLNVLAHILQKPYSQILAEFSDPKGRATTWDELGWTGDVKY
                     HMGAYRSLGNDKEVEMLVHMPPNPSHLEQIDPVIVGMARAANTKTDTAGPPRYFENES
                     LPIVIHGDASFAGQGIVAETLNFSHVPGYATSGSLHIIANNQLGFTATDGESRSSLHA
                     SDLAKGFEIPVIHVNADDPLACMEAARTAFAYRQKFRKDFVINLIGYRRYGHNEGDEP
                     RFTQPVMYQKIDEHPTIRQILADKLEEESLIEQGEAEELLQTHLKELQEANDQLDAEK
                     ALVEPVPQPPPRGAAQKVKTAITLKRLKELNRSLLQFPENFHLNPKLVRAVERRKSLF
                     EKSEEGKIDWATAEELAFASILEDGIPIRLTGQDSARGTFSQRHAVFYDAETNKAHIP
                     LQNIPQASAAFEVINSPLSEAGAIGFEVGYNIMAPDRLVIWEAQYGDFVNNAQGVLDE
                     LLFSSRAKWGLTPSLVLLLPHGNEGQGPDHSSGRIERFLGLAAEINVRIAYPSTAAQY
                     FHLLRRQALLLKTDPLPLVVFTPKGLLRHPLTASAGNQFTTGNWQRGIDDENLPGNKT
                     DVKNLILCSGRIYVDLVTSPLRKEHHDNAIARVEQLYPFPQEQLESLLAEYPKLESLI
                     WVQEEPLNMGAWNYLRPRLRALVEDRLPLHYVGRPEGSSPAEGSSTLYRINQQSLIEH
                     AFKLETQASTSSVVKERG"
     gene            9118..11238
                     /locus_tag="VFQ23_20280"
     CDS             9118..11238
                     /locus_tag="VFQ23_20280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9908999.1"
                     /translation="MKEKLPLKKSGKPVRKKRTVGNTGQPQIFNHERNPQFHKLLDAF
                     PVAVVMVGLNGMIQFINQKAKALLGAPDPSLKLEDWPQAFGLYLDDGKLTYPVQKLPL
                     SRALRGERVEESEEIILRREGEEKGIWISISTETLRDENGNVDGALALIRDISYRKTF
                     ELSREKQVQRIEALYRLSRVIAESGTELNKIAELTTEFTSTVIGDLSLMTLLNKTHEK
                     LNIVAFHDTDPTAQALLRKLLTPDFEFDYSQRLSGGVVKSGEPVLIPSIPSEQLRSIA
                     LPAFQEFIDVIGIESVIIVPLIGRGGILGTLNVSRHRGSKPLNLEDQSFLTDIAYRVA
                     LAIENCRLFESLRTEISEKLSTKRALDMSEERFRLIFESVTLGIKVLDHNGDILQTNP
                     AFQEMLGYAEDEFTGKHFTTFLHPLDISRASKLFQDVKSNGVTSFGFDHRTLHRDQSI
                     VWAKTFFTVVKKDDKDDEPAFIVGIVENVTEQKRLQFEMGELNSRLQNSMELERLHLA
                     QELHDNPMQVLYSANYRLEELRQKTDPEMGESLREVKDTIQGVLQDLRAMAKELRPPT
                     ISSFGLENAIRSHAFDVQEKHPNLQLYLSLAHDQQSLPDNVRLALFRVLQQALANVVH
                     HADATEVRIHFSFDAEETHLEIRDNGKGFTVPANWIEFVRQEHYGLAGAAERVHALGG
                     VFKVESSPGSSTTVRVTIPWSDPD"
     gene            11272..11937
                     /locus_tag="VFQ23_20285"
     CDS             11272..11937
                     /locus_tag="VFQ23_20285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909000.1"
                     /translation="MSDIRVLIVDDHPITCKGIRDLLEPAVGISVAGEAHSGAEALEM
                     IESLKPSVVLLDMKLPDMNGIEIIKRAYKIGTNARILGLSSYNDREFISQLLTYGASG
                     YLLKDEMPDEIINAVRGVAHGESGWVSRKVAAMLSQILLKEQEGGTELTSRELEVLGL
                     VVDGKTNNQIGLTLGISAKTVEKHLHAIFRKMGVVSRVEAAILAVQESLVKRDLNINA
                     QSE"
BASE COUNT         3012 a         2976 c         2984 g         3146 t
ORIGIN      
        1 cctgattgtg actgcaattc cagccagcgc aggatatctt tccggcgtag gaaaccgaac
       61 gtcctgtttc ttttaacgac aggtaattga tgaatgctac ggatttgcaa taggtcaaat
      121 gcttctgctg cagcatcatt cgcggcaaaa atgaatcaat ttctttgaga gcttcatgat
      181 gtttttgaca gtcctgctaa tgcaataaca ggccactcct catgtgcaat caatacctag
      241 ccctgataca aattgcttac attcattacc atctcgatct actttgtaga aatcaaaagc
      301 ccgcctccaa agagacgggc tgactgaaat tggaccagaa ccctttcaac ctgataaaag
      361 gtgatgagtt tacggaactc caaatctcaa ctgtgaatcg atattcgaat tgtcgtacgc
      421 tgggaacaag taggatgttg taaggtgcgc gaacggcaaa cctatcattt cagagtaact
      481 tgttccggcg ctcacatcga agtatgacac aatttctgag gcgatgatcg gctgtccatt
      541 catgctttga acaaggacag gcccgctgtc tatacccgca aaactgactt gctgtccctg
      601 gttaggctgg aggaggtaac tcccctgtaa gacaccgttg attgtcacgt tcactgttgt
      661 agacccggtg ccaacattca ccaaacgtag ttgtgagttg tgattcacat tgtcgtagac
      721 agggaagtaa taatgcgtgt cgagggaacc actgcgatgc cccatcgtct ccgaataatt
      781 agtccatgtg ctcccgttga agtgagcaaa gcgtatggat gcaacgatag gtacgctcgc
      841 tgaactctcc actctgaccg gacctccatt cagcgcgttg taggcaacct gaatagcctg
      901 attgggcgcc aatacataat tcccttgcac aacgccgccc accgctacgg ttacggtcgt
      961 cgagaagttc ccaatattcc ccaggcgcaa ttgtgcgtta aggttcacat tgtcatacca
     1021 gggcaggacg taactggtcc ttacctgccc gagcggcaag cccatcacgt ctgtccaggc
     1081 gctgccagtg ttatcggcga cctgcatcga agcaaggatg gagattccat ttgtgctctc
     1141 cactttgacc ggaccattat tcaaacccgc gtaactgacc agcaagcttc caccaggcgg
     1201 aacagaatga gtgccttgca caataccgcc gatgctgacc gtcactgccg catgtgagtt
     1261 tcccgggttt gcaacgcgta ggcgtgaatc atggttcaca ttgtcatacc acggaaacgc
     1321 gtagctggtg gcaagctgat tttccggaaa gcccatctgc tctgaatagc ctgtccacag
     1381 gcttccgttg tggttatgga caaatcgctg agatgcgaga atatttccgc cgttcgtgga
     1441 tagcacccgc atcatcccac tggcggtgta gctggcgcgc tcactcgact gcgctggtac
     1501 attgtatgaa ttttgaagaa tcccgccaac atagacttga acaggagtgg tggacgcagg
     1561 aacagggcca atgtaatttt gccgccgtga ttcgaaggcg tcgaagtaat aggttccacg
     1621 cgtgccagca tctattccgg tcagcgcgcc aagccggatc cgatcgattc gcttaccgct
     1681 gttgtccact ccagcaagag tcactttccc aatcccatct atccaaagtg tcagactgcc
     1741 gttagttgga cctgatgtgg tttcagcact ccaatccaac tcgatggagt gtgatccgtc
     1801 gctgatcgtg taccagtcgc tgtagaccca ggatgagtcg ttctgaaatg ctccagtcct
     1861 gatttggtaa agacccgcgg attgcctgaa ttccacacgc agtacttctg tcgacgttcc
     1921 cataaatcct ttgaagatga agtgtgcatc gccgctcgcc atcgagattg agtttggatc
     1981 aaaataaaag cggacacgat agcgaggttc ggcgttggga gaatcatcag tcacctggat
     2041 cgtgttggta tcgttgatca ccgcctgcaa gccttgtgct ccgacgagcg cggcggacga
     2101 gctaacactc aggtcacctc cgttaattgc actggcagtc caggcggaaa ggttgccaga
     2161 ttcgaagccg tcagcaaaaa tcaaatctga acttccgggt gtctgtgtta cgatgggcgt
     2221 gggtgtggct gtctgtgtgg gagtaacggt tggtgtagca gtcgcagtat gtgtgggtgt
     2281 ggaggctccc agcgtagcag tattggtcgg cgttgaggta gcagttgacg ttcgcgtttg
     2341 cgaaggtgtg gccgaagccg tgggcgtcgg ggtaccagtc ggtggcgaag tgaagcctga
     2401 tgcgaaaatg ctcattccat ctacatcatt agcagccagt ccccaggcac tgccatcaaa
     2461 ataaagtacg gatgagtaat tgcatgccgt gacattacca agggaggtag gcgcacaaac
     2521 aaagacatcc tcatctgcac ccgtcacgcc gttgactgag aaatcattta ctgtggaaag
     2581 atagaccttt ccattcagca catccagcgc gtccacatcc tcgccactgg tctctgccag
     2641 cccgacatct gagccatcga agtacatgga ccacgttcca ctggtattgt tgcccagaga
     2701 ggttgggaca aacgccagca catcttcatc tctacctcct gtaacacctg taaccacagg
     2761 gttgccagtt gttgagatga gcacacgtcc atcgagtagt aaatttacag agtcgatgtt
     2821 ctcagcactc acatccagcc caacatcaat gccattgaga tacatactaa aagcacccgc
     2881 tgtgttacta cccagggatg tggcttcaaa ccgcaccacg tcacgaggcg tcacagacat
     2941 gccattgagt gtaaaagatg tgttgaatgc catcaggatc gtatctgcat cgacgataga
     3001 gaacccaaat aagtccgaac cagctacgcc aacgtcagac ccgtcgaaga acaagctcca
     3061 gttcgtacca tcgaatttaa gaatatcttc atcagccgag gctaccccac caagggtttg
     3121 attccctgtc agagacaggt gaagaggcac atattcgaag gctccgatat cgcatccgct
     3181 tccctgtgga cgcgcaatcc cgcgttgatc tgcagccgga caatgcgtag tatttcctgc
     3241 attaagtgct ggtgaccctg gcagcagcgc catcgtttgc gttgagccgc cattgtctgc
     3301 taagggaccc aacatgggat cgcctgttag ggtgggctta cattcgggag aaccatcttc
     3361 gaccagatta ttgcctccat ttatgaagct attgtaaaaa gcgtaacaat ctccaccacc
     3421 ggcatgatta gcaaaaaggt tgttatacat ttttggcacc gatgcaccgg ggcccagata
     3481 aagattccca cccatcgttg ccgcgttgtt ggaaaacgta ttattgacaa gataataatc
     3541 caccgccgca cccaggtatg cgcctccacc ttcccctacc gcatcgttat cataaaatgt
     3601 attgttctcc aggatgatca gggtaggaat tgtgctggca gagaaataaa ctgccccggc
     3661 tcgtgcggtt gtggcatggt taccggcaaa caaattattc cgaacttcaa tcctggcatt
     3721 tcccaactcg ccaacgattg ctccaccagc ggcagcagaa ttgttttcga atagattatt
     3781 ttcgatcagg gaagtattcg tcgcgccgct aaaatagatt gtccctgcac ctcccgctac
     3841 attgttggag attacgttgc ctctcaagtt aagaatacct atgcttttta ctaaaatcgc
     3901 acctgcctcc acatcagatc tatttcctat aatctcgcta ttagagatat ttacaatcgc
     3961 atatggagag atataaatgg ctccagccga ataggcggta ttgtttgtta tgcgtacatt
     4021 ttcgagcgtg agagttgtat tattgccaac aaacatagct ccaccaaagt tagtgtaaga
     4081 tgagccagtc tgtttgcctt ctttcaaaac cacattcctg atggtgggtg cgatggttga
     4141 aggggcgtcg atgagaaaaa tcctgactgc atttccaccg ctaatctcga cgcgcggatt
     4201 caagcctacc ccatcaatga tcagattctt gttgatattc atttgtgatg ccagcataat
     4261 cgtctgacca gcaagagagg aatcaaagct gatcacatcg ccgggcgctg cattggcaat
     4321 tgcttgccgc aaagaacccg gaccgctgtc atttatattg atgacagtat gggtaacggc
     4381 ttgaactgtt tgcaccccaa acgctccccc catgagtgcc aaaatcagtg caaattgtaa
     4441 aataaatggc ttgcgatttt tagaaagcat gaactttcct cctgctcggt gtggtcattt
     4501 ctgttggcat gacacaacac ctgcttatat gttatccgtt ttcgcagcag agaaagttaa
     4561 gagatagtta cacttccctc aagcccctgt cgactctcag ggaatttagg aatattggcg
     4621 taaaaagagc ctgcctccaa tgagacaggc tcttgaacag acgtgagaat agatactgct
     4681 acccttccac cagcatccag gcggggtctt ccaataattg cttgatgtgc accaggaatt
     4741 gcaccgcctc gcgtccatct acaatgcgat ggtcgtagct caaggccaca tacatcatcg
     4801 ggcggatcac aacctgaccg ttctgcgcga ttggtcgttc ttcaattttg tgcaaaccca
     4861 aaatacccac ctgtgggggg ttgaggatcg gtgtactcat caatgaacca aacacaccac
     4921 cattggtgat cgtaaatgtc ccgccacgca gatcctcgat ggacagttta ccctggtccg
     4981 tcttcgccac aaaatccttc acttgttttt caatctctgc aaatgaaagt ttatcggcgt
     5041 cccgtatgac cggaacgacc aaaccctctg aggatccaat cgctgtacca atatcgtaat
     5101 aatgtttgaa gacgatgtct tcaccatcga tctctgcgtt aatttgcggg aaggctttca
     5161 acgcgctgac cgaggctttg acgaagaaag aggtgaagcc caatttaatg ccaaagcgtt
     5221 tctgaaattc ctcatttctc cggctgcgta tatccataac cgcgcccatg tctatctcat
     5281 tgaacgtggt tagcatcgct gtactttgcg aggcttccaa caagcgctgc gcgatcgtgc
     5341 ggcgcctgcg tgacatacgc gtccgttcct cgccgcgctc tgaggatgga gtcgtcgttg
     5401 ctgacaccac gggttgcgcc tcacgttctg cttccgctgg aacctggttg tcgctatcct
     5461 tttctttttc ctgcccttgg aggaatagtt ccacatcggc tttagtgacg cgaccttcgc
     5521 tgcctgtgcc agatacttta ctgagatcta catcgttggc gcgcgccaaa cgagaggcaa
     5581 caggtgtgac tggtggcggg gtttgacgtt gtgcttcgcc tttatcatta tttgaagcta
     5641 cgggaacggt ttcctcttcc ttttcaactt ctatttcttc tgtggctttg ggcgtaggag
     5701 cagatgatcc ttttggttct tctgccttct cttcgatcgt tcccaacaca tctccgactt
     5761 taacatcttt accttcagga acatcaatct tttgcaaaac gcccgatccc tttgcgccca
     5821 cctccaggtt tactttatcc gtttcgagct caaccagtac ttcgcctaca ttgacgaaat
     5881 caccctgctt cttgagccag cgtgatactg tagcgtccaa cacggattct cccaattcgg
     5941 gaaccaaaat ttttatcgcc atattattag cctctttcct tcaccacact ggatgtactt
     6001 gcctgagtct cgagtttgaa agcatgttca atcaaggact gttggttgat tcgatacaac
     6061 gtggaagaac cttccgccgg gctggaacct tcaggtctgc ccacatagtg taacggcagg
     6121 cggtcctcca cgagtgcccg caagcgtggg cgtagataat tccaggcccc catattgagc
     6181 ggttcttcct gtacccagat caaactctcc agttttggat actctgccag caaggactct
     6241 aactgttcct gcgggaatgg atacaactgc tcaacccgcg caatggcatt gtcgtggtgt
     6301 tccttgcgaa gtggacttgt caccaggtcc acatagatac gcccgctgca taagatgaga
     6361 ttctttacat ccgttttatt gcctggaagg ttctcatcat caataccacg ctgccagttg
     6421 ccggtagtaa attgatttcc ggcggaggcg gtgagagggt gtcttaataa ccctttcggc
     6481 gtaaagacaa ccaaaggtaa cggatcggtt tttagaagca gagcctgacg tctcagcaag
     6541 tggaaatatt gtgctgccgt ggatggataa gcgattcgga cgttgatctc tgcagctaaa
     6601 cctaagaaac gctcgattcg cccgctcgaa tgatctggtc cctgaccttc gtttccatgc
     6661 ggtaataaca aaaccagcga aggagttaat ccccatttcg cgcggctcga aaacagtaac
     6721 tcgtcgagca cgccctgcgc gttgttgacg aagtctccat actgtgcttc ccagatcaca
     6781 agtcggtctg gcgccatgat gttatagccc acttcaaagc caatggcgcc cgcctcgctc
     6841 aacgggctgt tgataacttc aaaagcggcg ctggcctgag ggatattttg caagggaata
     6901 tgcgctttgt tagtctcagc atcataaaag acagcatggc gctggctgaa ggttccgcgg
     6961 gcgctatcct gaccggtcaa gcgaatcggt atcccgtctt ccagtatgga tgcaaacgcc
     7021 aattcttccg cggtcgccca atcgatcttt ccctcttctg atttttcaaa taacgatttt
     7081 cgccgttcaa cggccctcac cagtttcggg ttcagatgaa agttttcggg aaattgcaat
     7141 agcgatcgat tcaactcttt gagacgctta agcgtaatag cggtcttgac cttttgagcc
     7201 gcgccgcggg gagggggttg tggaacaggt tccacaagtg ctttttctgc gtccagttga
     7261 tcgtttgctt cctgcaattc cttgaggtga gtttgcagga gttcttccgc ctcaccctgc
     7321 tcgatcaaac tttcctcttc caacttatca gccaatattt gtctgatggt cgggtgttca
     7381 tcgatctttt gatacatgac cggttgggtg aagcgcggtt catcgccttc attatggcca
     7441 tagcggcgat aaccaatcag gttgatgacg aaatccttgc ggaacttttg acggtacgca
     7501 aaagccgttc gagcagcttc catacaggcg agcggatcat cggcattgac atggatgacg
     7561 gggatttcga atccttttgc gaggtcactg gcgtgaagac tgctgcgcga ttcaccgtca
     7621 gtggcggtga agcctaattg gttgttggca atgatgtgta aactgccgct ggtggcatag
     7681 cccggcacgt gtgagaagtt tagcgtctct gccacgattc cctgacctgc gaaagatgca
     7741 tctccatgaa tcacaatcgg cagagattcg ttttcaaaat aacgcggcgg gccggctgtg
     7801 tccgtttttg tgttggcggc tcgtgccatt cccacaatga ccgggtcgat ttgttccagg
     7861 tgactgggat tgggcggcat gtggacaagc atttcgacct ctttatcatt ccccagtgat
     7921 cgataagctc ccatatgata tttgacatcc cccgtccagc caagttcgtc ccaggtcgtc
     7981 gcgcgtccct tggggtcact gaattctgcc aggatttgac tgtatggttt ctggaggata
     8041 tgagccagca cattcagccg cccacgatgg gccatgccga tgagcacggc acgaattttt
     8101 tcgtccgagg cagaggcaat gatttcatcc agcatgggga taagcatatc caggccttcg
     8161 atcgaaaagc gcgtcttgcc ggggtagatg cgatgaagga agagttcgaa ggactcgatc
     8221 tgggtcagtc gctccaataa ttgcttttca tccacgtttt gctggggagg gcgataatgg
     8281 cccgtctctg cagtttgata cagccagttt ctttcttctg gaatgcggat gtgtccgtag
     8341 tcgtagccga ttgttccaca gtaaatggct cgtaaagagt caattgcctg ctgcgcgttt
     8401 tcatctccat gttgattggg caacttcaaa atttcagccg gtagagtaaa taaatcctct
     8461 tcctgcagct tatgaaacgt gagcgttaat aagggatcct ctgctattgg gggagtgttg
     8521 ttcaggggat caagccgcgc tgagagatag ccgtaggaac gaatggacca ggccaggttc
     8581 actgctccag taacggaaag caggctgcga ggcgcggcaa gagctggtcc ggccccattg
     8641 ggcgaccagc ttgcaaacag cttccgagtt gcttcgtcta cagaagcagg atcatccaga
     8701 taccgttgat ggagttcgag aatgtatcca aaattgggtc cgtgaaaatc cttttccaat
     8761 gccatatcca aatcgtcctc actgtctcat ccttttgatg ggacagttat tttatctaag
     8821 attttttatt tccaccgcat cttttttacc acaaattaca gggtttttca aaatgaggtt
     8881 gttgcttgca aaacccgcgg cttctggtat tattgcatca tcatccggac tgacccccac
     8941 tccatccttt gataaaaata taatacaaga gttagactga cttaatctaa attcagtgtt
     9001 ttaacgtaaa atcctgtgaa catccggcgg gcccaatcat ccggttctac attggctcga
     9061 aattttgcgt gcgtctaggc acagttgttc gttattgtag agaagaggat cacacttttg
     9121 aaagagaaat tgcctttgaa aaagtccggc aaacccgttc gtaaaaagcg gaccgtgggt
     9181 aatactggac agccacaaat atttaaccac gagcggaacc cacaatttca taaacttctg
     9241 gacgcatttc cggtcgccgt tgttatggtg ggtttgaatg gcatgattca attcatcaat
     9301 caaaaggcaa aagctctgct gggtgcaccg gatccttctt taaagctcga ggattggccg
     9361 caggcctttg gcctatacct ggacgatggg aagttaacct atccagtgca aaagctgcct
     9421 ctgagtcgcg cattaagagg tgaacgtgtt gaagagtctg aagaaatcat tctgcgaaga
     9481 gagggggaag aaaagggaat ttggatttcc atatcgaccg agactctccg agatgaaaac
     9541 gggaacgtgg atggggcact tgctctgata cgagatattt cgtaccgtaa gacattcgag
     9601 ctttcgcgcg aaaagcaggt tcaacgcatt gaggcgcttt atagactgtc gcgtgtcatt
     9661 gccgaatcgg ggacagagct gaataagatc gcggaattaa cgacagagtt tacttcgact
     9721 gtcatcggcg acttaagcct catgacttta ctaaataaaa cgcatgaaaa actaaatatt
     9781 gttgcttttc atgacactga tccgacggca caagctcttt tgaggaagct cctgacacca
     9841 gactttgaat ttgattattc tcaaaggctt tcaggagggg tggtcaaatc tggtgaacca
     9901 gtgctgatcc catcgattcc gtctgagcag ctacgctcta ttgccttgcc tgcatttcag
     9961 gaattcatag acgtaatcgg tatcgaaagc gtcattattg tgcctctgat tggaaggggt
    10021 gggatattgg gcaccttgaa tgtatcccga catcggggca gtaagccttt gaatcttgaa
    10081 gaccagtcat ttttgaccga tatcgcctac cgagttgcgc tcgccatcga aaactgccga
    10141 ttgttcgaat cactacgcac tgaaatttcg gagaagctat ctacaaagcg ggcgttggat
    10201 atgagtgaag aacgctttcg gctgatcttc gaatcggtca cactggggat aaaggtgttg
    10261 gatcacaacg gcgatatttt gcagacgaat ccggcgtttc aggagatgct gggatatgcc
    10321 gaagatgaat tcaccggcaa acatttcact actttcctcc atccgctcga tattagtcga
    10381 gcttcaaagc tattccagga tgtgaaatcg aatggagtca ccagttttgg ttttgatcat
    10441 cgcactcttc accgagatca gtccatcgtt tgggcaaaga ctttttttac cgtcgtgaaa
    10501 aaggatgata aagatgatga gcctgctttc attgtcggca ttgttgaaaa tgtaacagaa
    10561 caaaaacgcc ttcaattcga aatgggtgaa ttgaatagtc ggttgcaaaa cagtatggaa
    10621 ctcgagcgtc ttcacctggc gcaggaactt catgataatc cgatgcaggt cctttacagt
    10681 gcaaactatc ggttggagga attgcgtcaa aagacagacc cggaaatggg ggagtcactg
    10741 cgagaggtga aagatacaat tcagggcgtg ttacaggacc tccgcgccat ggccaaagag
    10801 ctgcggccgc ccacgatttc cagttttggt cttgagaatg ctattcgctc gcatgcgttt
    10861 gatgtgcagg aaaagcaccc aaatctgcaa ctttatcttt cattagctca tgatcaacaa
    10921 agcctgccag ataatgttcg gctggcatta ttccgtgtgt tgcagcaggc gctggcgaat
    10981 gttgtccatc atgccgatgc aacagaagtc cgcatccatt tttcgttcga tgcagaggaa
    11041 acgcatctcg aaattagaga taatggaaaa ggctttactg ttcccgcgaa ctggatcgaa
    11101 ttcgtacgcc aggaacacta cggattggcg ggtgcagctg agagagtaca tgctctgggc
    11161 ggtgttttta aagtagaatc cagcccagga agttccacca ccgtgcgcgt aactattcca
    11221 tggagtgacc cggattgaat atatcaatcc tggataatga aagggtaaac aatgtcagat
    11281 attcgtgttt tgatcgtaga tgatcatccc attacctgta agggaattcg tgatttgctc
    11341 gaaccagctg tgggaatcag tgtggctggc gaagcgcata gtggtgccga ggcgcttgaa
    11401 atgatcgagt ccttaaagcc tagtgtggtg cttttggata tgaaactacc ggatatgaat
    11461 gggatcgaaa ttattaagcg tgcgtataag attggaacga atgcgcgcat ccttggttta
    11521 agctcctata atgaccgtga gtttatttcc cagttgctta cgtatggagc ctcaggatat
    11581 ttattgaaag atgaaatgcc tgatgaaatc atcaacgctg ttcggggcgt ggcacacggt
    11641 gagtcaggct gggtcagtcg taaagtagcg gcgatgctga gtcagatcct attgaaggag
    11701 caggaaggcg gcacggaatt aacctccaga gagttggagg tgctgggcct ggtcgtagat
    11761 ggaaagacaa acaatcagat cggcctgacg ctgggaatca gtgccaaaac cgtagaaaaa
    11821 catttacacg ccatattcag aaaaatgggc gtggtttctc gtgtggaagc agccatattg
    11881 gcagtgcagg aaagtctggt gaagagagat ttgaacataa atgcgcaatc tgagtaagtt
    11941 gacgccacgg cagaaagaac aagtagccta tttggagcat ttgctccagc acgcaagtac
    12001 tatacatttg taatactaca tagggaaatt ccccattcgg aattcctgcc gaatgtcata
    12061 aaatttgtat atgacgcgcc ttgatgtact aaatgtaaac cccaaggagg ctgttgac
//
LOCUS       DASTJP010000693         3828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1326305, whole genome shotgun sequence.
ACCESSION   DASTJP010000693 DASTJP010000000
VERSION     DASTJP010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326305"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VFQ23_20290"
     CDS             complement(<1..1574)
                     /locus_tag="VFQ23_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909001.1"
                     /translation="MNNFVDYESPKRSSSRRKRQDSILVILFGMFLSFLCAGVLGLAL
                     YLSGYNPFLFPSSLTATALAQRNASCQVLIDKAIQSSSGFCGQTGSNNVCYGNTTIQA
                     ELAPGVTQRFSERGDILPVGDLRRLTAAPLNLQTEEWGIAVFKVIANLPRSLPGETVT
                     MVVFGNTTIDKQSGGLESFYFFSELGQIACEAVPSDGLMLTSPDGGGLRINVNGTELT
                     LKGDANLKAVKNGEMEVSLFRGSGRIVSNGQEQYFGAGQQVSVDLGGENGVQSVSGPS
                     APEPLTQDELNTACSLTGQYCSQSQITPVSEAEAQGQLQAEITFTPTSPPTQTATIRP
                     SPTLFSTFTSFVLPSWTPSPGPPTLTRTPARTPTRTLTPTRTRTRTATPSRTPTRTNT
                     PTQTLTSTITSTFTPSATVTPTFTPSSTPVPPTDPTCSAQISLGALTNPNPNEIAAAI
                     TNTTGAPIAITRFFAYWVESPSSQKIDKLFLDTVEIWNRSDPNSPSDIPTEGNWISAA
                     LTIPGDGTAKDFLIQF"
     gene            complement(1597..2145)
                     /locus_tag="VFQ23_20295"
     CDS             complement(1597..2145)
                     /locus_tag="VFQ23_20295"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9909002.1"
                     /translation="MNLFDKFCPVCKRKNDRDAIACIHCGTLLDSYPMNETGLTRTTD
                     MTARVEERVGELLIDESVIPEGGIAIYLAGTSDPVYLSSQQEFVIGRKVGEEEVPDDF
                     LDLAKWGGYQLGLSKRHAMVRRIDRGYEAIDLSSRNGTWLNDDQLIPEKPYSFVSGSL
                     FRLAKMRLLVVYRPILTTQQKM"
     gene            complement(2422..2700)
                     /locus_tag="VFQ23_20300"
     CDS             complement(2422..2700)
                     /locus_tag="VFQ23_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909003.1"
                     /translation="MESVSARMEIRVGVTDGGTRVTGTIAAVEVGRADGCIDVGAGAP
                     GVDIISTEKVQAFRNIIATVKLQINLKNRRFFNEGVECSGCVMQDSHL"
     gene            2714..3640
                     /locus_tag="VFQ23_20305"
     CDS             2714..3640
                     /locus_tag="VFQ23_20305"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9909004.1"
                     /translation="MNIGDGNVLRTVAFTPDSAVLASAGGNDQDFAVRLWDVGSGLSI
                     GKLEGHTGIVWGVAFSPNGQMLVSVSSDKTAKIWDWNTATLLHSIDLPGEVVSAGFSP
                     DGQFLTVGGVDEPVGDVQHAAIWAFFVGSWNPFLKFPEYWNITAMAYSPDGRYLIGGG
                     TSRNVQIWRTSDGTSLYTLNHPHQVFDAAVSPDGSTIATATCQNTLNEECTEGAVWLW
                     NSSNGRLLDRDRLRYFPDVVESVTYSVDGSTLIAASRDGTIRIYAASDYQLLFEATPP
                     GGNGVMALSADGGLLATGGANGSVHLWKVVYP"
BASE COUNT          977 a          920 c          938 g          993 t
ORIGIN      
        1 tggaattgaa taagaaagtc ttttgccgtt ccatcacccg ggatggtgag tgctgcactg
       61 atccaatttc cttcagtagg aatatcactc ggcgaattcg gatctgacct gttccaaatc
      121 tcaaccgtgt cgagaaaaag tttatcgatc ttttgagagg atggcgattc cacccaatat
      181 gcaaaaaatc tggtgatggc aatcggagcg cctgtggtgt tagtaatagc cgctgcaatc
      241 tcattcggat tgggatttgt cagggcacca agtgatatct gcgcgctgca ggtcggatca
      301 gtgggaggta caggagtaga tgatggagta aatgtaggtg taaccgtggc tgagggcgtg
      361 aaggtcgaag ttatggtgga ggtaagcgtt tgagtaggtg tattcgttcg cgttggtgtt
      421 cgagaaggtg tggcagtgcg cgtccgtgtc ctggtaggag tcagagtccg cgtcggtgtg
      481 cgcgccggtg tgcgcgtcaa ggtaggtggg ccaggcgacg gagtccagga gggtaataca
      541 aagcttgtaa atgtggagaa aagtgtaggt gatggtctga tcgtagcagt ttgcgtcggc
      601 ggcgatgtag gagtgaatgt gatctcagcc tgtaactgtc cttgcgcttc tgcttccgag
      661 acgggtgtaa tctgactttg cgaacaatac tgacctgtca gcgagcaggc ggtattgagt
      721 tcatcctgag tcagcggctc gggcgctgag ggtccactca ccgattgaac gccattctcg
      781 cctcccaaat caacactgac ctgttgacct gcaccaaaat actgctcctg cccattcgaa
      841 acgatcctgc ccgaaccacg gaagaggctt acctccatct ccccgttttt aacagccttc
      901 aggttggcat cccccttcaa ggtcagttct gttccattga cgttaattcg aagtccaccc
      961 ccatcaggcg aagtgagcat cagtccatcc gacggaactg cctcacaggc aatttgacca
     1021 agctcactga agaaataaaa cgattccaaa cctccacttt gcttatcaat ggttgtatta
     1081 ccaaatacca ccatcgtgac agtttcacca gggagagaac gaggcaaatt cgcaataact
     1141 ttgaagaccg caatacccca ctcttcggtc tgaaggttta gaggcgcagc ggtaagacgg
     1201 cgcaagtcgc caacgggcag gatatcgccg cgttccgaaa accgttgcgt gacccccggc
     1261 gctagttctg cctggatggt cgtgttaccg taacagacgt tgttcgagcc ggtctgcccg
     1321 cagaagccgc tggatgattg aattgcctta tcaatcagaa cctggcaact ggcatttctc
     1381 tgagccagag ccgtcgccgt caggctggag ggaaaaagaa aaggattata gccactcaga
     1441 tacagagcca atcccagcac gcctgcgcac agaaaagaca ggaacatacc aaatagaata
     1501 acaagaatcg agtcttgccg ctttcgacgg gaggaggatc gcttgggact ttcgtaatcg
     1561 acaaagttat tcataaaaga ttatctacaa gtatatctac attttctgtt gcgtagttaa
     1621 tatcgggcgg tacacgacca acaatctcat cttggccagc cggaatagtg agccactgac
     1681 aaaagaataa ggcttctctg ggatcaattg atcatcattc aaccaggtac catttcgact
     1741 ggacagatca atggcctcat atccccgatc gatgcgcctg accatcgcgt gccgttttga
     1801 taatcccaat tgatatccac cccactttgc gagatcaaga aaatcatccg gaacttcctc
     1861 ttcccccacc tttcgaccga tcacaaattc ctgttgggac gataaataaa cagggtctga
     1921 agtacccgcc agataaatag caattccacc ttccggaatt acggactcat caataagtaa
     1981 ttcaccgacc cgctcttcga ccctcgctgt catgtcagtg gttctcgtca gccccgtctc
     2041 gttcatgggg tacgagtcaa gcaacgtacc acaatggata caagctatgg catcgcggtc
     2101 attcttacgc ttacaaacag ggcagaattt atcgaataaa ttcaaaatat ttctcctgaa
     2161 caatgagtct tccaagagtc aggtgctgct taaatcttac accgtgtaat ctaaaatttc
     2221 aacacattaa acaaggcaat ttcattctaa atcgtttagt ctatgaaagt tttaacaaat
     2281 tggcaaggat ttgtctgtac gagtagccat tttatttttt tgtccgaaac tagcgccgat
     2341 tgattcttgc tcaatcatgg tttttatttt atgcttgcga tattcaatcg gcaagtgccg
     2401 caaccaaaac gctttcttga attatagatg ggaatcttgc atgacacatc cggaacattc
     2461 aacgccttca ttaaaaaaac gccgattctt gaggttgatt tgtagtttta cagtggcgat
     2521 gatgttcctg aacgcctgca ccttctcagt ggaaataatg tccacgcctg gtgctccagc
     2581 gccaacgtca atacagccat ctgctctgcc aacctctaca gcagcgattg ttccagttac
     2641 tcttgtgccg ccatctgtga cgcccactct gatttccatc cgggccgata cactttccat
     2701 gctggagatt ttcatgaaca tcggggatgg aaatgttttg cgcactgttg ctttcacacc
     2761 tgatagcgcg gtccttgcat ccgcgggtgg caacgaccag gattttgccg tacgtttgtg
     2821 ggacgtcgga agtggactct cgattggcaa attggaaggt cataccggca ttgtctgggg
     2881 tgtggcgttt tcaccaaatg ggcaaatgct ggtctcagta tcaagtgaca aaactgctaa
     2941 aatctgggac tggaacacgg caacgttgct tcattcgatt gatttgcctg gagaagttgt
     3001 cagcgctggc ttttcgcctg atggtcagtt tctgaccgtc ggaggagtgg atgagcctgt
     3061 aggtgatgta cagcatgccg ctatctgggc tttttttgtg ggatcctgga acccgtttct
     3121 taagttcccc gagtattgga atatcacggc catggcatac tcaccggatg gcagatatct
     3181 gatcggcggc gggacctctc gtaacgtcca aatctggagg acaagcgatg gtacatccct
     3241 ttacactctt aatcatccac atcaagtttt cgatgcagct gtttcacccg acggctctac
     3301 tatcgcaaca gcaacatgtc aaaatacatt aaacgaggaa tgtacagagg gcgctgtttg
     3361 gttgtggaat tcgtccaatg ggagattgct cgatagagac cgactcaggt attttccgga
     3421 tgtcgtcgag agcgtgacat attcagtaga tgggtcaaca ttgatcgccg catcgcgaga
     3481 tgggacaatc cgaatttatg ctgcttcgga ttatcaactc ctgttcgaag cgacaccacc
     3541 aggtggaaac ggagtcatgg cgctttcagc agatggtggt ctcctggcta ctggtggagc
     3601 caatggatca gtacatcttt ggaaagttgt gtatccttga taaggccgcg tcaattgtaa
     3661 gtatcgacgc ggtacgttcg gtctagtaat caagtcttgt taatttgcca acttgactca
     3721 gaacaaatgt tctaatataa ttccacgaag gtcaaagcaa aaagaccaat tgaatatacc
     3781 cgacgtcctg gctacccatt agatactttg aaccgaaaac ttcagcca
//
LOCUS       DASTJP010000694         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_313731, whole genome shotgun sequence.
ACCESSION   DASTJP010000694 DASTJP010000000
VERSION     DASTJP010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313731"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VFQ23_20310"
     CDS             <1..1279
                     /locus_tag="VFQ23_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909005.1"
                     /translation="GEAMKPAMDSSIRADVGSEPVVINLTSGNVWLRPLSSVSTHEWD
                     CESIYWSRELGIIINQQQYGTTFGPVKEAVITYTFDGQQVTEYWGKIHGVSPRGEKIL
                     VDEDTIIDLRNNKITDLAWHMNYDVGDSSTLYWSSDETRLYRCCFYYADLKTGKGYNF
                     AWSDLRGMDGEPLTIPMHPHQYGHWVRNDSYFLIEWDYWTILYGDPIPMFSPSEKKYY
                     DIAKIAVIPASVVSTSTSDVSPDGMHVWIKGYGDDGQYHGFLINLVDFNTVSYDKPVD
                     EFEWSPDSKYAWMTIYGTSDIYVLSAAEKTLTLFPADSISDLFWRPNDHVVAFFTEQD
                     QVLTFLDVKHMSIKEWKLPASFDNLFWSPDGSHMALIAVDGSLWQVEYPKLENLEQLT
                     PPLSNVYSLNWSPDGNSVSFISGSDLYVVKTDK"
     gene            complement(1304..2236)
                     /locus_tag="VFQ23_20315"
                     /pseudo
     CDS             complement(1304..2236)
                     /locus_tag="VFQ23_20315"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
BASE COUNT          607 a          529 c          516 g          613 t
ORIGIN      
        1 aggcgaggcc atgaaacctg ccatggattc gagcatacgg gcggatgtag gctccgagcc
       61 tgtcgtcatt aatcttacga gtggtaacgt ctggttacga ccactatcaa gtgtatccac
      121 gcatgaatgg gattgtgaat caatctactg gtcacgagaa cttggcatta ttatcaacca
      181 gcaacaatac ggaactactt ttggtcctgt aaaagaggct gtaatcacct atacattcga
      241 cggacagcag gtcactgaat attggggaaa gattcatggt gtatcgcctc gtggtgaaaa
      301 aatcctggtg gatgaagaca cgattattga tttgcgcaat aacaagataa ccgacctcgc
      361 atggcacatg aattacgatg ttggggattc atcaacactt tactggtcat ccgatgagac
      421 tcgcctgtac cgatgctgtt tttattatgc tgatcttaag acaggtaaag gctataactt
      481 tgcatggagt gacttgcgcg gaatggatgg agaaccattg accattccca tgcacccgca
      541 tcaatacgga cattgggtac gaaatgacag ctatttttta attgaatggg actactggac
      601 tatcctgtat ggggatccaa ttcccatgtt tagtccgtct gaaaagaagt attacgacat
      661 tgcaaaaata gctgtgattc cagcatccgt tgtttccacg tctacatcgg atgtgtcacc
      721 cgatggaatg catgtttgga taaaaggata tggagatgac gggcaatacc acggctttct
      781 gattaatctg gtcgacttca acactgtctc ttacgacaaa cctgttgatg aatttgaatg
      841 gtcaccagat agtaaatacg catggatgac tatttatggc acaagcgata tatatgttct
      901 ttcggctgct gaaaaaacat tgacactttt tcccgcagat tcaataagcg acctgttttg
      961 gcgccccaat gaccatgttg tggctttctt cactgaacaa gaccaagtgt taactttttt
     1021 ggatgtcaaa cacatgtcca taaaagaatg gaaattgcca gcctcttttg acaacctttt
     1081 ctggagtcca gatggcagtc atatggcgct gatagcggta gatggaagtt tatggcaggt
     1141 tgaatatcca aaattggaaa atttggaaca gctcacgcca ccactatcga atgtgtatag
     1201 cctcaattgg tcaccagatg gaaattctgt gtccttcatc agtggttcag atctttatgt
     1261 tgtgaaaaca gataagtgag ataacaaacc ctgccaaggc tacctaccca gacaattcct
     1321 tcaagatctc ctctgtatgc tcgcccaatc tcggcggcgg tagtttataa ctcactgggg
     1381 tttcaccgaa ttttagtggt gagccaacca gtctcaagtc tccaatctcg ttatgctcca
     1441 catcgatcaa catttctcgc gcttcgactt gtggagtagc gaagactttt tcaagtgtat
     1501 tgatcgatcc gcatgggatc cctgctgcat caagcatgga taaccattcc ctggatgtct
     1561 ttgtgagaaa gattggcttg aggatggaaa tcaattcctc gcggtttttg acacgttctg
     1621 aatttagacc aaagcgcgca tcatttacca attccgaatt accaattact tcacacaact
     1681 tctaaaactg cctgtcattt cccaccgcga ccacaaacca cgcatcgctg gcttgaaagc
     1741 tctgataagg aacaatattg gcatgggcat tcccgtatcg ttttggcaaa ttgcctgaga
     1801 tcaaataatt gctggcgaca ttggcaagca tcgccacttg tgaatcaaat aaggaaatat
     1861 caagatgctg ccccttgccc gtcaaactgc gcgcgtgtat cgcggcaaga atggcaatca
     1921 cggcattctg ccctgcgaac aggtctgcca ccgccacgcc gactttcatc ggctcgccat
     1981 ggggctcgcc ggtgatactc atcaatccgc ccatagcctg gatcatgaaa tcatagccgg
     2041 gtttgtcgcg cagcggtccc gattgcccga agccggtgat cgagcaatag atgaggcttg
     2101 cgttgattct ggcgacagac tcaaaatcaa tggaataacg tgccagcgtc ccagggcgaa
     2161 agttctcgac cagtacatcg cttttcttga tcagctcatg gagagttgtt cgtccctgct
     2221 cggttttgaa atccacgacg atactgcgct tgttacggtt gacac
//
LOCUS       DASTJP010000695         4767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_797827, whole genome shotgun sequence.
ACCESSION   DASTJP010000695 DASTJP010000000
VERSION     DASTJP010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797827"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFQ23_20320"
     CDS             complement(<1..679)
                     /locus_tag="VFQ23_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909006.1"
                     /translation="MKHLFFSNAPKKGMQALLALTIVLISLGPGGVRIAYAAPPANDD
                     FNFAKVINTLTYGDTLVTTEATPTTTIPNVDDPDNINCDGNTISAGYSTVWYRYTPGA
                     TESIALNTAGSNYDTFIAVWTGTRGNLNLVACNDDTFAQFTSDLFFIGTSGTTYYIEV
                     AKFNGVPAESCDIKPGGCSLQFNAFISTTNVSIHGTVVGNYSIPTGGSLRTGYPGIDD
                     GPAKITNT"
     gene            complement(974..1852)
                     /gene="rfbD"
                     /locus_tag="VFQ23_20325"
     CDS             complement(974..1852)
                     /gene="rfbD"
                     /locus_tag="VFQ23_20325"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET9909007.1"
                     /translation="MKIVLFGKDGQLGWEFQRILPVLGEVTALGRRDADVSDLNTIEK
                     ILGELNPDLIINASAYTEVDKAETQVDLAITVNATAPGVMAEMARKLNAVFIHYSTDY
                     VFDGNNTIPYKETDPANPLSVYGQSKLNGEQNVQQAGDAYLILRTSWVYSLRGNSFVN
                     KVLGWARKNETLKIVSDQISSPTWARMLAEITSLVIAPSKAVLFDSIRERRGLYHLAG
                     SGYTSRYEWAKQILANDQNLEEQLTHTLEPGLTSDFPTPATRPLFSALDCARFRENFG
                     LQMPDWNTMLNLAMAE"
     gene            complement(1862..2407)
                     /gene="rfbC"
                     /locus_tag="VFQ23_20330"
     CDS             complement(1862..2407)
                     /gene="rfbC"
                     /locus_tag="VFQ23_20330"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET9909008.1"
                     /translation="MNIVSTSIAEVLLIEPKVFQDERGFFLESYQKNHFREAGIDVDF
                     VQDNHSKSRYGTLRGLHYQLKHPQGKLVRVIAGEIFDVAVDIRRSSPTFGKWVGDYLS
                     AENKKMLWVPAGFAHGFFVTSPSAELLYKATDYYAPEWERTIAWNDPTIKITWPLQNE
                     APVLSAKDKTGKPFVDAEVFE"
     gene            2564..4087
                     /locus_tag="VFQ23_20335"
     CDS             2564..4087
                     /locus_tag="VFQ23_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET9909009.1"
                     /translation="MLARVFSCAVVGLEGVIVEVEVDYTNGLPGVTIVGLPDAAVVES
                     RERVQTAVKNAGLHFPRHRIVVNLSPASIRKEGPAYDLPIALGVIILAGFLPHDVIEN
                     TIVIGELSLDGMVRHTRGVLPMAATARANGFKRMFVPEVDAPEAALIPDLEVIPVRSL
                     ADLYAHLSGRELIEPYQPSGDELEPLFVPTDFGEIKGQEHVKRALEVAAAGGHNALMT
                     GSPGSGKTLLARAMPGILPEMSIEESLDVTRIYSVADQLPAGTPLIRHRPFRAPHHTI
                     SHAGLVGGGNIPKPGEISLAHRGVLFLDEFPEFGTRVLEVMRQPMEDKIVTISRAKGS
                     LTFSANFQLIAAMNPCPCGYYGDTQKPCSCAPAVVTKYQKRISGPLLDRIDIHIEVPR
                     VDYEKLSADRVGESSEAIRSRVQAARDRQSKRFANNESGIVCNADMRVGEIRQFCKLQ
                     DEGQSLMRAAMSQMQLSARAYHRILKLARTIADLAGCAEIQSVHLAEALQYRPKILS"
     gene            4474..>4767
                     /locus_tag="VFQ23_20340"
     CDS             4474..>4767
                     /locus_tag="VFQ23_20340"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9909010.1"
                     /translation="MWLAQYSQEQTLQVARELLNLPQPPTAIFSATDFQALGVLKADP
                     LVLKIPGQLTVIGFDDLDMAEYADLTTISQHLDESGRLAVEILLGQIASPSRPP"
BASE COUNT         1137 a         1164 c         1191 g         1275 t
ORIGIN      
        1 tcgtgtttgt gatctttgcc ggtccatcat cgatgccggg atacccggtt cgcaaactgc
       61 cgcctgtcgg aatcgagtaa ttgcctacca ctgttccatg aatgctgacg ttcgtggtgg
      121 agataaaggc gttaaactgc aggctgcaac cgccaggttt tatatcgcag ctttctgcag
      181 gtacaccgtt gaatttcgcc acttcaatat aataggttgt tcccgaggtg ccgatgaaaa
      241 atagatctga cgtaaattgt gcaaaggtat catcattaca agcgaccaga ttaagattgc
      301 ctctcgtccc tgtccatacg gcaatgaagg tatcatagtt cgaaccggca gtgttcaagg
      361 cgatggattc agtcgctcca ggtgtatatc gataccaaac ggttgaataa ccggcagaaa
      421 tggtatttcc atcacagtta atattatctg gatcgtctac attggggatc gtagttgttg
      481 gggtggcttc cgtcgtaaca agagtatccc catacgtaag ggtattgatg acctttgcaa
      541 agttaaaatc gtcgtttgca ggtggagcgg cataagcgat gcgaacgcct ccaggtccaa
      601 ggctgatcaa cactatggtc agagccagta gggcttgcat cccctttttt ggtgcgtttg
      661 agaaaaacaa atgcttcata acgtcctccg aggaattaac aagaaaacaa taaagctcga
      721 cgatgcgaga atagaacttt actagacaat tatatctttg ctgctcaggg tgaatagatt
      781 tttcttaatt attatagtta acaaactgga aagtttttat tcccaaaaag tactgctgca
      841 aaactccctg acaatctgct tccgtcacag ctgcacctaa accagaccat ggtttcgatt
      901 cttgcgaacc gtgatcttca gttggtttgc ggctgtttga tttccccgct gaaatgaatt
      961 cgatgaagta gcgctattca gccattgcca gatttaacat cgtattccag tccggcattt
     1021 gcagcccaaa attttctcta aagcgcgcgc aatccaacgc tgagaaaaga ggacgcgtgg
     1081 caggtgtggg aaaatcactg gtcagacctg gctcgagggt atgtgtgagc tgttcttcca
     1141 gattctggtc attcgcaagg atttgtttcg cccattcgta gcgagatgta tatccactgc
     1201 cggcaagatg gtatagccct cgcctttcac gaatcgaatc gaaaaggact gctttcgatg
     1261 gcgcgatcac aagactcgtg atctctgcca gcatccgtgc ccaggtgggg ctactgattt
     1321 gatcactgac aatcttcaac gtttcatttt tacgcgccca cccgagtacc ttgttgacaa
     1381 aactgttccc gcgcaggctg tagacccagc tcgtccgcaa gatcagataa gcatcgcccg
     1441 cctgctgtac gttttgttcc ccattcagtt tgctctgtcc atagacgctc aatgggttcg
     1501 ccggatcggt ttccttatag gggatggtat tgttcccatc aaacacatag tctgttgaat
     1561 agtggataaa gactgcattc agtttgcgtg ccatttcagc catgactccc ggcgcggtcg
     1621 cgttgacggt tatggctaga tcaacttgtg tttcagcctt atctacttcg gtataagccg
     1681 aggcattaat gatcaggtca ggattgagtt cccccagaat cttttctatc gtgttgagat
     1741 cagaaacatc cgcatccctt cggcccaaag ccgtcacctc acctaaaaca ggcaggatgc
     1801 gctggaactc ccagcccaat tgaccatctt tgccaaacag gacgattttc attctcaatg
     1861 attattcaaa tacttcggcg tctacaaacg gtttcccggt tttatccttt gccgataaga
     1921 ccggtgcttc gttctgcagg ggccaagtga tcttgatggt cgggtcattc caggcaattg
     1981 tgcgctccca ttccggagcg taatagtctg ttgctttgta gagcaactcg gcggatgggc
     2041 tggtaacaaa gaaaccatgt gcaaagcctg ctggcaccca caacattttt ttgttctccg
     2101 cagataaata atctccgacc catttgccaa acgttggtga gcttcgtctg atatctacgg
     2161 caacatcaaa tatttccccc gcgatgacac ggacaagttt tccctgcgga tgtttgagtt
     2221 gataatgtaa gccgcgcagc gttccatatc gtgatttgga atgattgtcc tgtacaaaat
     2281 cgacatcgat gcccgcttct ctaaaatgat tcttctgata actttcaaga aaaaatcccc
     2341 tctcatcctg gaatactttc ggttcaatta acaagacttc ggcgatcgat gttgaaacaa
     2401 tattcaaagt aatttcctaa actggaatgt gcctcgttga gatgttcccg atggacgggc
     2461 atattatagc cgcgtttgat tggcttttcc tctggcacga agtatctgtt agttatccaa
     2521 tctgtgtgtt gacattttaa tctgcctcat ctacactgcc cgcatgcttg cgcgtgtttt
     2581 ttcctgtgcg gttgttgggc tcgaaggtgt catcgtcgaa gtggaagtgg attacaccaa
     2641 cggtctgccc ggtgtaacga tcgttggctt gccagatgcc gcagtggtgg aaagccgcga
     2701 acgcgtgcag acggcggtca agaacgcggg gttgcatttt ccacgccatc gcattgtggt
     2761 caatttatcg cccgcttcca ttcgcaaaga aggccctgca tacgatctgc cgattgccct
     2821 gggcgtgatc atcctggcgg ggtttctccc gcatgatgtg attgaaaata cgatcgtcat
     2881 cggtgaactc tcgttagatg gaatggttcg tcacacgcgc ggcgtcctcc cgatggcggc
     2941 cactgcccgc gccaacggat tcaagcggat gttcgtgccc gaagtggacg cgcctgaagc
     3001 ggccttgatt cctgacctag aagtgatccc tgttcgatcg cttgcggatc tttacgccca
     3061 tttatctggt cgcgagttaa ttgagccgta tcaaccctct ggcgatgagc ttgaaccctt
     3121 gttcgtccct acggacttcg gcgaaataaa agggcaggaa catgtcaaac gtgcacttga
     3181 agtggcggcg gctggcgggc ataacgcgtt gatgacgggc agccctggaa gcgggaagac
     3241 tctattggca cgcgccatgc caggcatcct gcctgagatg agcatcgaag aatcgctgga
     3301 tgtgacgcgc atttattcgg tcgccgatca actgcccgca ggcacacctt tgatacggca
     3361 tcgtccgttt cgtgcgccac atcatacgat ttcacacgca ggtcttgtgg gtgggggaaa
     3421 tattccgaag cccggagaaa tatcgcttgc acacaggggt gttttgtttt tagatgagtt
     3481 ccccgaattc ggcacgcgcg tgctcgaagt gatgcgccag cctatggaag ataaaatcgt
     3541 cacgatcagc cgcgcgaaag gatcgttaac atttagtgct aactttcaac tcatcgccgc
     3601 gatgaatccc tgcccgtgtg gttattatgg tgacacgcaa aaaccatgtt cttgcgcgcc
     3661 agcagttgtg acaaaatatc agaagcgaat ctcaggaccc ttgctggacc gaattgacat
     3721 tcatatcgaa gtgccgcgtg tggattacga aaagttaagt gcagatcgag taggtgagtc
     3781 gagtgaagcg attcgatccc gcgtgcaagc tgcgagggat cgacagagta aacgtttcgc
     3841 gaacaacgaa tcagggattg tctgtaatgc agatatgcgc gtgggggaga tacgtcagtt
     3901 ttgcaaattg caggacgaag gtcagagttt gatgcgggca gccatgagtc aaatgcaatt
     3961 atcggcgcga gcgtatcacc gaatcctgaa gttggcgcgc acgattgcgg atttagcggg
     4021 gtgtgcggaa atccaatctg tgcatctcgc cgaagcctta caataccgcc ccaagatcct
     4081 ttcgtaaatt tcaggatagg cagttacttt atgagatgca ccggcgctcc aagtgccggt
     4141 gtgggagatg cgggcttgag gcccgctcgc ccgagcagag gcgctacgat atcggccgaa
     4201 gttgatcttg ggataggaaa gtttatctgc ggtatccact ccgatttgcc cgagaaatct
     4261 gttttctttg aggatgacct ctttattgac gtacttatag tgtctatgtt ggtctgaaag
     4321 tgctttcctt acatctttca ttgtagatct cgtgtgcctc cacaaaaagc aataccaatc
     4381 tgcctgagta ttccattcac cctgtacgtt ttcgcttcag agggtttcgg cgagctctaa
     4441 cacaagcccg catcaaatta ccggaaggca attgtgtggc ttgctcagta ttctcaggaa
     4501 cagacacttc aggttgccag agaattgttg aaccttcccc agccgccaac tgcgattttc
     4561 tcggcaacag atttccaggc attaggggtg ttaaaggctg atccgttggt cctcaaaata
     4621 ccaggacaat tgactgtgat tggttttgat gacctggata tggctgaata cgctgacctt
     4681 accactattt cccaacatct ggatgagtcg ggcaggttgg cggtggaaat attactgggg
     4741 caaattgcat ccccatctcg accaccc
//
LOCUS       DASTJP010000696         4297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_798703, whole genome shotgun sequence.
ACCESSION   DASTJP010000696 DASTJP010000000
VERSION     DASTJP010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798703"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..755)
                     /locus_tag="VFQ23_20345"
     CDS             complement(12..755)
                     /locus_tag="VFQ23_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909011.1"
                     /translation="MKKILLIGCLILSACQTATVAPTPTLVPLPTETPLPTAIPATDT
                     PVPTPTIAFSPTPLPRFFTNEFDSAFEGWGILQAGNDFVPNVRAENSTLLLQMDAPYS
                     WVYAIYGSESYSNVYVETKFENRAGSPASVGLVCRYSEENGWFEYNVTSDGTYNLLYG
                     QWLATGVADYLPITDGTSNLVQPSGAAQQISLLCADTVLTLSINGTVVRSLDVARYEL
                     TEGKVGVTTASYENTPILVALDSVTVREP"
     gene            complement(934..1764)
                     /locus_tag="VFQ23_20350"
     CDS             complement(934..1764)
                     /locus_tag="VFQ23_20350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909012.1"
                     /translation="MISEHNAVCTFYKKLLTFYPENFRERLGESMEQTFRDLWNEKRQ
                     TKKELLGFVLRTFLETAMGIFREHLLLISPGDIMQNVFKAIGLPALLSLLLIFPFIVI
                     EVVNRRNFNEDFPTFLFLVMWLDLFALIILLLPIVQSRRTRNRDMANVLPTQSTLLTK
                     PAWAVIISIMVFISPGILPLMESFGWLSLDRLFNGPNPEVDYLPGMFLSLGFLLFPVV
                     AGIIAGGPIVRTLRAGGSLFAHPVNLIIVVFIASTFAFGLVTLIIDQWPCFVGVPNCD
                     "
     gene            complement(1761..2117)
                     /locus_tag="VFQ23_20355"
     CDS             complement(1761..2117)
                     /locus_tag="VFQ23_20355"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9909013.1"
                     /translation="MTTTNANPPLTPAVFHILLALSSGERHGYGIMKQVEADSQGKVS
                     MGPGTLYGSLKRMLDAGLVIESDKRVDPEMDDERRIYYQITGMGAQALEAELGRYKRI
                     VTIAQQRKLFPKSFDV"
     gene            complement(2146..3015)
                     /locus_tag="VFQ23_20360"
     CDS             complement(2146..3015)
                     /locus_tag="VFQ23_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909014.1"
                     /translation="MIRSTLRIATILVVVILIMVYPFLPGQYDALAFSFSTVVQLLAT
                     VGLLLVPLGIVWLVYEFWKRGRIDRSLPFENRSYSFAIVSLIVSSLLAILVSLVAAFG
                     IGMSFGVLTLVLWLYVVSRLIPGLKLMKTAKAEIINPAPFYLILIPLILLLFQLTLAA
                     PLTEFSRNRAMANSHEFITDIEAYQDEYGHYPVSLVAMWKDYYPDVVGVEKYHYLPYG
                     ESYNLFFEQPRFLLDNFGTREWVVYNPQDEQRVYSHTSWFLLLTPEELETSQGWYDVH
                     DAASPHWKYFWFD"
     gene            complement(3095..3482)
                     /gene="ssrA"
                     /locus_tag="VFQ23_20365"
     tmRNA           complement(3095..3482)
                     /gene="ssrA"
                     /locus_tag="VFQ23_20365"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3609..>4297
                     /locus_tag="VFQ23_20370"
     CDS             3609..>4297
                     /locus_tag="VFQ23_20370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909015.1"
                     /translation="MGEKILIIDDDLDTLRLVGLMLQRQGYQISAATNGQQGLDKAFE
                     EDPDLILLDLMMPEMDGYEVTRRLRQNPSTIETPILMFTAKSQLDDKVIGFEVGANDY
                     LTKPTHPTELQARVKALLARMSEKRGGTAPVKNENHGYVIGVLGARGGLGTTTLAVNL
                     AAGLQTRARSEVIVVEMLPGQGALALDMGISSSKGLVDLLSISKLSDITREKVRDSLA
                     NHPSGIKFLLAS"
BASE COUNT         1198 a         1014 c         1078 g         1007 t
ORIGIN      
        1 gagagaggaa tctatggttc tcttaccgtc accgaatcca gtgcaacgag aatcggagta
       61 ttttcataag aggccgtagt gactcccact ttgccctcgg tgagttcata gcgtgccaca
      121 tccaggctgc ggaccacggt cccattgatc gaaagggtta ggactgtatc ggcacacaaa
      181 agactaattt gctgagctgc tccactgggt tgaactaaat tcgaagttcc atctgtaatt
      241 ggaagataat cagcaacacc agtcgccagc cactggccat agagaaggtt atatgtccca
      301 tcgctggtga cattgtattc aaaccatccg ttctcttcag aataacgaca aaccagccct
      361 acagaagccg gactcccagc gcggttttcg aatttcgttt cgacataaac attcgaataa
      421 ctctctgagc catagatagc gtaaacccat gaatagggtg cgtccatctg aaggagaagt
      481 gtgctatttt ctgcccttac gttgggaaca aaatcattcc cagcctggag gatcccccag
      541 ccttcgaacg ccgagtcaaa ttcgttcgtg aaaaatcgtg ggagtggagt tggagaaaac
      601 gcgatcgttg gggtgggaac aggggtgtcg gtcgcaggaa ttgccgtcgg cagaggtgtt
      661 tcagttggga gaggtacgag ggtaggcgtt ggtgccacag tcgccgtctg gcaggcactg
      721 aggataagac atccaataag aagaatcttt ttcatagtgc ttattaaaac acaaacctct
      781 ggaagtttgt tccagaggtt gtgttttacg cacaatatgt ctactaatca atcgcaaggg
      841 atgatgagga gcagactgat gagcgccggc gaccggagag tcaggaacat agttttcata
      901 gcagcacctc cttcaaatca tattgttgcg agattaatcg cagtttggga cacccacaaa
      961 acagggccac tggtcaatga tgagggtaac caacccaaaa gcaaaggtgg acgcaataaa
     1021 gacgacgatg atgagattga cgggatgcgc aaacagactg cctccagctc tcagggtgcg
     1081 gacgatcgga cctccggcaa tgatccccgc aaccactggg aacaagagga aaccaagaga
     1141 gagaaacatt ccaggaagat aatccacctc agggtttggc ccattgaaaa gacgatccaa
     1201 tgacagccaa ccaaaggatt ccatcaaagg gaggatgccg ggggaaatga aaaccataat
     1261 gctaatgatc acggcccagg cgggtttggt aaggagagtg ctctgtgtgg gaaggacatt
     1321 cgccatgtca cgattcctgg ttcgcctgct ttgcacaatc ggcaacaaga gaatgatgag
     1381 ggcgaatagg tccagccaca taactaaaaa taggaaggtg gggaaatctt cattgaaatt
     1441 tcgccggttg acgacttcta tgactatgaa tggaaagatt aggagcaaac tgaggagcgc
     1501 gggtaatcca attgccttaa atacgttttg cataatatct cccggagaaa ttagcagcag
     1561 atgctctcta aaaatcccca tggcagtttc aaggaaagtt cgaagtacaa agccaagcag
     1621 ttcttttttc gtttgccgtt tttcattcca aaggtctcgg aacgtctgct ccatcgactc
     1681 gccaagccgc tccctgaaat tttccgggta gaaggtaagc agtttcttat aaaaagtaca
     1741 aactgcgttg tgttcagaga tcatacgtcg aaggattttg gaaacagctt ccgttgctgt
     1801 gcgattgtga cgatcctttt ataacggcca agctctgcct caagagcctg cgcacccatc
     1861 cctgtaatct gataatagat gcgccgctcg tcatccattt caggatcaac gcgcttgtcg
     1921 ctttcgatga ccagcccggc atccagcatg cgtttgagcg acccatacag cgtgcccggt
     1981 cccatcgaca cttttccctg cgagtcggct tcgacctgtt tcatgattcc ataaccgtgc
     2041 cgctctccgc tggagagggc taatagaata tggaacacgg cgggtgtaag aggtgggtta
     2101 gcatttgtag tagtcataat caatccattt ttttctggtt gcggtctaat caaaccagaa
     2161 atatttccaa tgaggactcg cggcgtcatg gacgtcatac catccctgac tcgtttccag
     2221 ctcttccggc gtaagtaaca aaaaccagga ggtgtgactg tatacacgtt gttcatcctg
     2281 cgggttatac acgacccact cccttgtgcc aaaattgtcg agtaagaatc tcggttgttc
     2341 aaaaaagaga ttgtatgact cgccatacgg caagtagtga tacttttcaa cacctaccac
     2401 atcaggatag taatctttcc acatcgccac aagagaaacc ggatagtgtc catattcgtc
     2461 ctggtacgcc tcaatgtccg taatgaattc gtgactattt gccatcgcgc ggtttctact
     2521 gaactcggtt aagggcgcag caagtgttaa ctgaaaaagg agtaggataa gagggataag
     2581 aatgagataa aacggcgcag gattaatgat ctcggctttc gccgttttca ttagttttag
     2641 tccgggtatt agcctggaga caacatacaa ccataaaaca agggtaagaa cgccaaagga
     2701 catgccaatg ccgaaagcag cgacgagcga aacgagtatg gcgaggagcg acgatacgat
     2761 cagggaaacg attgcgaaag aatagcttcg attttcaaat ggaaggcttc gatctattcg
     2821 ccctcgcttc cagaattcat acaccagcca tacaatcccc agaggaacca acaacaaccc
     2881 aactgttgcc aacaactgaa ctacggtcga aaaagagaac gccagtgcat catactgacc
     2941 cggaaggaag ggataaacca taatcagtat gaccacaacc aggatcgtgg cgatgcgaag
     3001 agtagatcta atcattttca gactatatcc gcgacggata tagttgtcaa gagcattgtt
     3061 aaagcaaaat gtccagcaat gctggacatt tataaggtgg agatggcggg aattgaaccc
     3121 gcgtccgaaa gactcgaccc tcggaaatct acgagcgtag cctgccgaag tttgtcatca
     3181 cagggatctc ggcgggcagg taaccctgtg accatctgct agtcttaagc acatttagca
     3241 gagtcatgtg cagcactcac accattgttt cgcccgatcc gtcaccgggt ggaggacggg
     3301 gccggcggac gcaacatcaa ctgtggatgt tgactgttat gatctcatcg cattaagcgg
     3361 cgagcggcat agcagcgtag ttagtgcgat tggcacttaa tggtttgcgc tgagtttacg
     3421 agttcggcgc ctctcggctc gcattccggg accagcctct cccgtcgaag cctgtcatcc
     3481 ccgggtagtg cactttacat tgcaactaca atttcattat agcatactta caagccgcct
     3541 atttatcttg ctctaaacat cgctttatgc tagactgttg tcaataataa gaggaaaaat
     3601 atactattat gggcgagaaa atcctcatca ttgacgatga cctggacacc ttacgattgg
     3661 tcggcctgat gcttcaaagg caggggtatc agatcagcgc cgccaccaat gggcaacaag
     3721 ggctggataa agctttcgag gaagaccctg atttgattct gctcgatttg atgatgccag
     3781 agatggatgg atatgaagtc acgcgtcgat tgagacagaa tccatcaacc attgaaactc
     3841 ccattctgat gttcacagcc aaaagccagc ttgacgataa ggtgattggt tttgaagtag
     3901 gagccaatga ttatctgacc aagcccacac atccaaccga actgcaggcg cgtgtaaaag
     3961 ccctgcttgc ccgcatgagt gaaaaacggg gcggaaccgc gccggtaaag aatgaaaatc
     4021 atggctatgt gatcggtgtt ttaggtgctc gaggcggttt gggcacaact acgctggcag
     4081 tcaatctcgc cgccggtttg caaacgcgcg cgcggagtga agtcatcgtg gtagagatgt
     4141 tacccgggca aggcgcactg gccctcgaca tgggcatctc aagttcaaaa gggttggtcg
     4201 atttattaag catcagcaaa ttaagtgata tcactcgcga gaaggtgcgt gattcactcg
     4261 ccaatcatcc ttcaggtata aaatttctgc tggcctc
//
LOCUS       DASTJP010000697         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_818357, whole genome shotgun sequence.
ACCESSION   DASTJP010000697 DASTJP010000000
VERSION     DASTJP010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818357"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /gene="cobU"
                     /locus_tag="VFQ23_20375"
     CDS             <1..508
                     /gene="cobU"
                     /locus_tag="VFQ23_20375"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HET9909016.1"
                     /translation="SGKSSYAQSLAEQTGESVTFIATAQAFDEEMSARIQKHRAERPV
                     AWETLEIPIDLASHIQKIKSEVIILDCVTLLASNLLMQFVKDDLVEEAPFKLAIQTEV
                     EGLLDAIRGQEQDWIIISNELGLGLVPPYQMGRVYRDWLGWANQRLAREADQVIFMVA
                     GIPMVVKG"
     gene            517..1053
                     /locus_tag="VFQ23_20380"
     CDS             517..1053
                     /locus_tag="VFQ23_20380"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909017.1"
                     /translation="MHIQIRQMDGQSIQQVDQFNRNSMVNSHLLLHVQDNKISYSIVP
                     VEPYKKILTVDAEDYTTFIDSSQKVIFFANVDGKPVGQIKMVPWWNKFAYVEELTVDT
                     EFRGKGIGHSLLTHAVDWAKHQGFPGVTLETQDNNVSACKLYEKCGFVLGGFDSYVYK
                     NFPENQGEIGLYWYLIFS"
     gene            1092..1892
                     /locus_tag="VFQ23_20385"
     CDS             1092..1892
                     /locus_tag="VFQ23_20385"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909018.1"
                     /translation="MLKIQSLSVSYGPRQILHNISLDVQSGEVLALIGPNGAGKSTLI
                     RAVSGVIPISSGNVHTNGDNFATLSTIQRARYVATVPQAVSLPPAYTVWETVLFGRTP
                     YLGFLGQPSKKDEDIARQSLSRVSALPFAERRVGELSGGEQQRVLLARALCQTTPILL
                     LDEPTAHLDLQYQVNLLELVSELAHKDNLAVLVALHDLNLAAHYADRIALMVAGNIKA
                     IGKPREVLQPELIAEAYCLPVQVVKHPFLDIPLVLPESGSYPSHKTSS"
     gene            1979..2638
                     /locus_tag="VFQ23_20390"
     CDS             1979..2638
                     /locus_tag="VFQ23_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909019.1"
                     /translation="MKKVHQQPVERKMDEKIKGGISLWDRITNDPEFMKLKREIQARY
                     GLPLNYDIRLNNQDWSNWLGDDDRPSSQKSKRGQAFLDDVTALFKKFQIPDAWHNDLF
                     AEIAGRSYSTAEEIWGTPKFEIYRDGDGNWKWQCIVTPETDLTNPVNLHLIKEQQKAW
                     AGDPPKPVKDKSNQRKLDWRPVYEWYKRHPLFTVEEIAKKIGYPAQRVKLRFSEFETK
                     E"
     gene            complement(2747..>3324)
                     /locus_tag="VFQ23_20395"
     CDS             complement(2747..>3324)
                     /locus_tag="VFQ23_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909020.1"
                     /translation="SRSVEILETFPNVGMVTARPFRTPPEFYESTLKWAQKDAVLEDG
                     QFIPWERFLEFNLSLGQTEEENRKVYAETKDWRITYKGITAMAGASHWQFTAYKSTLQ
                     QFLPFEMDKPMGQVRQLDKRMNDAGLLRLMVSDPLAMNMSNTLGYLRGELKGGSKKKR
                     VALGKRVAELSPIKKLLLAIYNRIFKIYYDE"
BASE COUNT          903 a          733 c          799 g          889 t
ORIGIN      
        1 cagcggaaaa tcatcgtacg cccaaagtct tgcagaacag actggcgaat cggtgacctt
       61 tattgccacc gcccaggcat ttgatgaaga aatgtctgcc cgcatccaaa agcatcgcgc
      121 agagagacct gtcgcttggg aaacgcttga aatcccgatt gatctcgcat cccatatcca
      181 gaagataaaa tcggaggtga tcattctgga ttgcgtcact ttgctggctt cgaatttact
      241 gatgcaattt gtgaaggatg atcttgtaga agaagcgcca ttcaaattgg ctattcaaac
      301 ggaagtggaa ggattactcg atgccatccg cggtcaagaa caggattgga ttattatctc
      361 aaatgaactg ggactgggat tggtcccgcc atatcagatg gggcgcgttt atcgcgactg
      421 gctgggctgg gcaaatcaac ggctggcgcg tgaagcggac caagtcattt ttatggtggc
      481 tggcatcccc atggttgtaa aaggataagg tcgtttatgc atattcagat tcgtcaaatg
      541 gatgggcaaa gcatccagca agtggaccag ttcaaccgca attccatggt gaattcgcat
      601 cttctccttc acgtccagga taataaaata tcctattcga tagtccctgt ggagccgtat
      661 aaaaagatac ttaccgttga tgctgaggat tacactacat tcattgatag ctcacaaaag
      721 gtgatctttt ttgccaatgt ggatgggaaa cccgttggcc aaatcaagat ggttccctgg
      781 tggaataaat ttgcttatgt cgaagaactc actgtggata ctgaattcag gggcaaggga
      841 attgggcatt ccttactaac ccatgcagtc gattgggcga agcaccaggg tttccctggc
      901 gttacgcttg agacgcagga taataacgtc tctgcctgta agttgtatga aaaatgtgga
      961 tttgtacttg gaggatttga ttcatatgta tacaaaaatt tccccgaaaa ccagggtgag
     1021 attggtttgt attggtattt gatattttcg tagggacagg cagctgcctg tccctacgcg
     1081 gaaataaaaa aatgctcaaa attcagtctc tctctgtctc ttatggtccg cgccaaattt
     1141 tgcacaatat ctctctggat gtgcaaagcg gtgaagtact cgcgctgatc ggaccgaatg
     1201 gcgcgggtaa atccaccttg atccgcgcgg tgagtggggt gattcccatc tcaagtggca
     1261 acgtccacac gaacggcgat aacttcgcga ccctttcgac gattcaacgc gcccgctatg
     1321 ttgccactgt tcctcaggct gtttctttgc cgccggctta tacagtttgg gagacagttc
     1381 tctttggacg tacaccttat ctgggatttc ttggtcagcc ctcaaaaaag gatgaagata
     1441 tcgctcgcca atcgttgtcc cgcgtgagcg cgcttccttt tgcagagcga cgcgtcggcg
     1501 aactctcggg cggagagcaa caacgcgtat tgttagctcg tgcgttgtgt cagaccacgc
     1561 ccatcttgtt actcgatgaa ccaactgctc atttggattt gcaataccag gtcaatttac
     1621 tggaattagt atcagagctt gcccacaagg ataatctcgc agtgttagtc gctttacatg
     1681 atttgaatct ggctgcacac tacgcggatc gcattgcctt gatggtcgcg gggaatatca
     1741 aagctatagg gaaaccgagg gaagttttgc aaccggagtt gattgcagaa gcctattgcc
     1801 tgcctgtgca agtggttaag catccgtttt tggatattcc gctggtgtta ccggaatctg
     1861 gtagttaccc ctcgcataaa acatcttcat agaccaatcc gagaggtggt attatttcta
     1921 tatgaaattt caattgccca tctctcagga agagggccgt ttgttttaga tttctgaaat
     1981 gaaaaaagta catcaacaac cagtagaacg aaaaatggac gaaaaaataa agggtggtat
     2041 atcactatgg gataggataa ctaatgatcc tgaattcatg aagttgaaac gagagataca
     2101 agcccgttac ggcttgcctt taaactatga tattcgtctc aacaatcagg attggagcaa
     2161 ctggctaggc gatgatgaca ggcccagcag tcaaaaatca aaacgcgggc aggctttttt
     2221 agatgatgta acggctttgt tcaaaaaatt tcagatccct gacgcgtggc ataatgatct
     2281 ttttgcggaa attgcgggac gctcttacag tactgctgaa gaaatatggg gtacgccaaa
     2341 gtttgagatt tacagagatg gtgatggcaa ttggaaatgg caatgtattg tcactccaga
     2401 aacggattta acaaatcctg taaatcttca tcttatcaaa gagcagcaaa aagcctgggc
     2461 tggtgatcct cccaaaccgg ttaaagataa gagtaatcag cgcaaattgg attggcgacc
     2521 cgtttatgaa tggtacaaac gacacccact ttttacagtg gaagagatcg ccaagaaaat
     2581 aggatatcct gcccagagag taaaattgcg gttttcggaa ttcgaaacaa aagaataggc
     2641 aacataagca gttgcgttga tgatcgcaca acaaatattc tcacaagcca acaaatgtct
     2701 tgggagttct gttaacagga gtagggcggt gttggcgacg ggtcttctat tcgtcgtagt
     2761 agattttgaa gatcctgttg taaattgcca gtaataattt cttgatcgga gataactctg
     2821 ccacgcgttt cccgagtgcc acgcgtttct ttttacttcc acctttcaac tcgccgcgca
     2881 aatagcccag cgtatttgac atattcatcg ccagcggatc agagaccatt aatcgcaaca
     2941 agcccgcatc gttcatacgc ttatccaact ggcgaacctg tcccattggc ttgtccatct
     3001 caaatggcag gaattgctga agcgtagatt tataagcagt gaattgccag tgagaagcgc
     3061 cagccattgc agtgatgcct ttataggtta ttctccaatc tttcgtctct gcatacacct
     3121 ttcgattctc ttcttccgtt tgcccgagtg agagattgaa ctcaagaaat ctttcccacg
     3181 ggataaattg cccatcttca agcaccgcat ctttttgtgc ccacttgagt gtggattcat
     3241 aaaactcagg cggggtgcgg aatggacgcg cggtcaccat accaacgttt ggaaacgttt
     3301 cgagaatttc gacagaacgc gaaa
//
LOCUS       DASTJP010000698         8657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_587822, whole genome shotgun sequence.
ACCESSION   DASTJP010000698 DASTJP010000000
VERSION     DASTJP010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587822"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFQ23_20400"
     CDS             complement(<1..756)
                     /locus_tag="VFQ23_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909021.1"
                     /translation="MIPLEHFLQLPTDEVAALVRTAGQKVCVFPVNGTRRWFMLEHGD
                     KISNDFIEAYMEISIQNHAELCAMLFDHGIDTILAPVFGRELMNRGDEYTKRVGIDGL
                     VRTATDKHYREFFDNYKVRVRFYGDYQKILAGTPYEYALESMREVTESTQSHSGFRLF
                     FGVFADDATETIARLSVDHYLAHNAIPDKQTLIQKYYGEDLPSVSLFIGFDKFSVFDM
                     PLLSTGEEDLYFSVSPSPYMTERQLRSILYDHLY"
     gene            complement(753..1340)
                     /locus_tag="VFQ23_20405"
     CDS             complement(753..1340)
                     /locus_tag="VFQ23_20405"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9909022.1"
                     /translation="MLTGLIQAVITLLTVGIFYTIDSVLIRHYDRHRQASGSGRAWDF
                     TLFIFAMVAALVLQPILLPIISFRTDQLWGLAAQVLGILTIIGSFALHIWSRAHLQHF
                     YAERVEVQPEHKVIDTGPYALMRHPVITSFFGIATGLFLINPALTTLAALLYTVWDFM
                     SAAKAEEDLLTKTLPGYADYARRTPRFLPRLGGGR"
     gene            complement(1347..2375)
                     /locus_tag="VFQ23_20410"
     CDS             complement(1347..2375)
                     /locus_tag="VFQ23_20410"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HET9909023.1"
                     /translation="MTGLQVKTFFSIKEIETEVWDQLSGCLPFQSHRWYRFGERVLSD
                     CPPVYLLVYDAGNLVARASLWLVRNEPLPKMPVPVKLLIASLLKRWPLLICRSPLANT
                     AGIILKDNSNRAAILAALTSAATDQARRQKASIVLFDFLRESEMGEWPSDFAQLKMPS
                     AGTMLRNKWQSLEEFLAEGNKKDRQHYKRTLREADKLGIRLSQHRIVHEPDRALALIQ
                     NVEDRYSSTHNPWMRAMLENIDMVNGTWLEAHINNRFVGCGLILEDRDVQLTTGLGLA
                     ENTPYVYFRLIYASLEAAFTKKVELLRWGTGAYEVKKRLGFELEQNNNSVLFGANPWT
                     RFIIKLAT"
     gene            complement(2501..3430)
                     /locus_tag="VFQ23_20415"
     CDS             complement(2501..3430)
                     /locus_tag="VFQ23_20415"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9909024.1"
                     /translation="MNKKVPPQKGSRTLKRIIVLGVMRSGTSLTAELIRLWGAFAGPP
                     RQLWESNVNDPRGYGYMEYIPLQNLNNEILDNNDRVPPLAEVLEQKISQRTYQEQGEN
                     LLQTMDNLALENGAEEWVWKDARLPLTLPFWSNLWGDVIYVITIRHPAEIALSLAQAA
                     EIDKDNLPYSAGFTYWQYCMLQVISFTQQSKRKIFIAFDQLINNSEQECTRLSNFLDE
                     HCEKGSEDSDKRIAAMLAHVAKNQQHQHYQTPLAEMPQATREQRALYDFLRVKIQYPD
                     EACNINDFALYPGWREYLESVDMLMTLQGAKDM"
     gene            complement(3427..4476)
                     /locus_tag="VFQ23_20420"
     CDS             complement(3427..4476)
                     /locus_tag="VFQ23_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909025.1"
                     /translation="MAELAVQTFKSITEIDPEIWNRVVSGRGFQSHSWYRFGEHVMAE
                     CQPTYLLALDGDTPIAGAALFRVHNEPLPLPSIARRFMASVLKRRPLLVCRSPLADTS
                     AMLLPGEPVRDAALEVLAKTAQQEFKRQRCSFLVFDFLLTEQLRYPGWPPGCEPITVS
                     EPGTYMPIEWDSFETYLKSRNKNERQYFKENLKLAETSGLSLTRHKEVPDVESALTLI
                     QNVSIWNGSALNPWIRGLLTNFSMIDGTWLQIHKDGQFVGCAATMRDNRFQLTTALGL
                     EDDVPGGYFFLLYAALQEAFEQNVRLVRFGSGAYDVKRMLGLHLEDTNHAMATIAGTT
                     SRISSKVKSPAPSRK"
     gene            4566..4961
                     /locus_tag="VFQ23_20425"
     CDS             4566..4961
                     /locus_tag="VFQ23_20425"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HET9909026.1"
                     /translation="MENYTFRLKSAQDLFDSIEAFAREQQIEAGCVLSGIGSLTHFTV
                     RLADREIPSEYEGPFEIVSLAGTVSIYGSHLHISASDGDGRTIGGHLVPGSKIYTTAE
                     IVLAAFPGTVYKREFAEDSGYDELAVYKK"
     gene            complement(5005..5802)
                     /locus_tag="VFQ23_20430"
     CDS             complement(5005..5802)
                     /locus_tag="VFQ23_20430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909027.1"
                     /translation="MKIEIRDLHFTYPTGVQALRGVTLDIASGEQVAIVGQNGAGKTT
                     LVKHLNGLLTPTEGSVRIGGWDTNVQPVAKLARRVGYVFQNPDEQLFSKNVETEVAFG
                     PRNLKLDGEQVKSLVKDALEMTELSDKMEMNPYDLSPTWRKMVALASVISMDTDIIIF
                     DEPTTGQDAMSIARIANAIRTLRQRGKTLITITHDIDFCAENFERVIALSQGKVLLDG
                     KANDVLGQEEILATTYVEPPQLTRLGKRLGLGKTVRTQEEFLAALKK"
     gene            complement(5799..6650)
                     /locus_tag="VFQ23_20435"
     CDS             complement(5799..6650)
                     /locus_tag="VFQ23_20435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909028.1"
                     /translation="MAIVNLQNVTYKYPLTNAPVLKNINLKVEDGEFVAIVGPNGAGK
                     STLCYAIAGFVPHFFKGEMTGTVEVLGRDSQNSSLHEWVLNVGLAFQNPFNQISGAKY
                     TVFEELAFGLENIGIPRDEMMARVEDAMKLTGISELANRSPYSLSGGQQQRVALTSIL
                     VMQPKVLVLDEPTSQMDPIGTREVFIAIQKMAERGMTVILVEHKVEWIAHYVDRVIAL
                     DDGQVLLEGTPDEVLTSPLVEESGFGISRYTSVARKAQKMRLWQSDSLPITLDEAVDG
                     FSKVVAK"
     gene            complement(6638..7417)
                     /locus_tag="VFQ23_20440"
     CDS             complement(6638..7417)
                     /locus_tag="VFQ23_20440"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET9909029.1"
                     /translation="MHERLSFFIKRDSPIHRLNPMTKVVLAFSLVLIAFTSPWYWTPL
                     AIFIFVIIPLTVIGKVAPEYWSFFFKVILPTVLFLFVMQALFLPGESKVVFQIVGLEI
                     TELSIRLAFRNASRITVMVSSFTIMLLTTHPSELMSDLTRRGLPGQFAYVIISSLQII
                     PQMQAKAQTIISAQRSRGLDTQGSFIKRAGSLVPLLGPLVFGSLVEVEERAIAIEARG
                     FTSTKAKTSLHEIPDTSLDRTMRWTLAALVIISLALRLWLL"
     gene            complement(7508..8233)
                     /locus_tag="VFQ23_20445"
     CDS             complement(7508..8233)
                     /locus_tag="VFQ23_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET9909030.1"
                     /translation="MRETAMQSQKSTSWLNIGKDFNTMTWVLMPVAIAINMAVGQIVA
                     TLKLPVYLDSIGTVLVGVICGPWAGALTGALANTILGLTLNPDFLPWWPVAAVIGLVS
                     GICANYGLFKSWWKVVVTGFFIALAAAMSSVPISLLVYGGITTGGTGLITAFLVESGR
                     GIVQAVFASSFLTEPVDKITTAMLAFAIIQGLSKRFIARFPRAENVETDEKVSQTQLI
                     IAVVVVVLLILFAIFLLQRLTGG"
     gene            complement(8383..>8657)
                     /locus_tag="VFQ23_20450"
     CDS             complement(8383..>8657)
                     /locus_tag="VFQ23_20450"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET9909031.1"
                     /translation="GIEGCVINDPLALALTFAPQLCDYQELPVDVDISGGISTGKTLA
                     DFYNYGKKPANVKVALGVRPRDFIDLFVERMETLAQSNQNRRQPSN"
BASE COUNT         2169 a         2139 c         2124 g         2225 t
ORIGIN      
        1 atagagatgg tcatagagga tcgagcgtaa ttgccgttct gtcatatatg gagaaggact
       61 gaccgaaaaa tataaatctt cctcaccagt agataacaac ggcatatcga acacgctaaa
      121 tttatcgaag ccgataaata agctgacgga gggcaggtct tcgccgtaat atttctggat
      181 gagcgtttgt ttgtctggta ttgcattgtg agcaaggtag tgatctacgg agagtctcgc
      241 tatcgtttct gtcgcgtcat cggcaaacac gccaaagaac agtcgaaaac cagaatggct
      301 ctgtgtcgac tctgtaactt cccgcatgga ttcaagtgca tattcatagg gtgtgcctgc
      361 caggattttc tgatagtcac cataaaatcg gacccgcacc ttatagttat caaagaattc
      421 gcggtaatgc ttgtctgttg ctgtgcgtac caatccgtct atgccaacgc gttttgtata
      481 ttcgtcgccc cgattcatca gctcacgccc aaagaccggg gcaaggatcg tatcgatgcc
      541 atgatcgaaa agcatggcgc acagttcagc atgattctgt atggagattt ccatataggc
      601 ttcgatgaaa tcgttgctaa tcttatctcc atgttcaagc atgaaccagc gtcgtgtccc
      661 gtttacgggg aagacacaga ctttttgtcc cgcggtccgc acaagcgcgg ccacttcatc
      721 tgttggtaac tgcagaaaat gctcgagcgg aatcatcggc ctcctccaag gcgcggcaag
      781 aatcggggag tccgacgcgc ataatcagca tatccgggta atgtttttgt taacaggtct
      841 tcctcagcct tcgcagcact cataaaatcc caaactgtgt agagtaaggc tgccagggtc
      901 gtcagcgcgg ggttaatcaa aaataaaccg gtcgcgatcc cgaagaacga agtgatgacc
      961 ggatgacgca tcaaggcgta gggtccagtg tcgatcactt tgtgttcagg ctgaacctcc
     1021 acacgttctg catagaaatg ttgaaggtga gcacgtgacc aaatatgtag agcaaacgat
     1081 ccaatgattg tgagtatccc cagaacctga gcggccaatc cccagagttg atcggttcga
     1141 aaactgatta tgggcaggag aatgggttgt aatacgaggg cggcaaccat tgcaaagata
     1201 aagagcgtaa agtcccaggc ccgtcccgaa ccgctggcct gtcggtggcg atcataatgg
     1261 cgaatcaaaa ccgaatctat agtatagaag ataccgacgg taagaagtgt gataactgcc
     1321 tggatgagtc ctgttaacat atctgcttat gtggccagct taatgatgaa acgcgtccac
     1381 ggattggctc caaacagaac tgaattgttg ttctgctcaa gttcaaatcc taaccttttt
     1441 tttacttcgt aggcgccggt tccccaacgg agcagctcga ctttctttgt gaaggctgct
     1501 tcaaggctgg cataaatcag ccggaagtag acatagggtg tattttctgc caggcccaac
     1561 cccgttgtca actggacgtc tcgatcttcc aagatcaaac cacaaccgac aaatctgtta
     1621 ttgatatgtg cttcgagcca ggtaccgttg accatatcga tgttttccag catcgctcgc
     1681 atccagggat tgtgcgttga cgaatagcgg tcttctacat tttgaatgag tgccagcgcc
     1741 cgatctggtt cgtggacgat tcgatgttga ctcagacgaa tgccgagctt atccgcttcg
     1801 cgcagggtgc gtttgtaatg ctgccgatct ttcttgttgc cctccgccag gaactcttcc
     1861 aggctttgcc acttgttgcg taacatggtc cctgcgctag gcatcttcaa ttgggcaaag
     1921 tcggaaggcc attctcccat ttcgctttct ctcaggaaat cgaacaaaac aattgacgct
     1981 ttttgtcgcc tggcctggtc ggttgcggcg gaggtcaaag cggcgaggat cgctgctcgg
     2041 ttcgagttat cttttagaat aatgcccgct gtatttgcca agggcgagcg acaaatcaaa
     2101 aggggccagc gctttaagag tgatgcaata agcaacttaa caggtacagg cattttgggc
     2161 aaaggttcat tgcggaccag ccacaaggat gctcgtgcca ccaaattgcc tgcatcgtaa
     2221 acaagcaagt acactggtgg gcaatcagac agaacgcgct cgccaaacct gtaccagcga
     2281 tggctttgga aaggcaggca cccgctgagc tgatcccaaa cctcagtctc aatttcttta
     2341 atgctaaaaa aagtttttac ttgaagacca gtcatgatca aaattgcagc ttacgtctaa
     2401 cagaaagcga cgttcagaat agttttgtca gataagatct ggtttctcgt ccaggtgcgc
     2461 acgttcggca tgcctgcgac acattcgtaa cttgctgtgg tcacatatct ttcgcgcctt
     2521 gcaaagtcat gagcatatcc acgctctcaa ggtattctct ccaccctggg tatagtgcaa
     2581 aatcattgat gttacaggcc tcatctggat attgaatctt tactctgaga aaatcataaa
     2641 gagctctttg ttcccgagtt gcctgaggca tttctgccag tggtgtttga taatgttgat
     2701 gttgctgatt ttttgctacg tgggcgagca tcgctgcaat tcgcttgtct gaatcctcgc
     2761 tgcccttttc gcaatgctcg tccaggaagt tggataaacg ggtacattcc tgctcggaat
     2821 tattgatcaa ttgatcaaag gcaataaata ttttgcgctt gctttgctgt gtaaatgaaa
     2881 ttacttgaag catgcaatat tgccaatagg taaacccggc ggaatacggg agattgtcct
     2941 tgtcgatctc tgccgcctgt gctaatgaga gtgcaatttc tgccggatgc ctgatggtaa
     3001 tcacgtagat aacatcgccc caaagattac tccagaatgg cagcgtgagt ggaagacggg
     3061 catccttcca aacccactcc tccgctccat tttccagagc caggttgtcc atagtttgga
     3121 gaagattttc accctgttcc tgatatgttc tctgagatat tttctgctct aaaacttctg
     3181 caagcggagg gactcgatcg ttattgtcca aaatctcatt gttcagattt tgaaggggta
     3241 tatattccat atacccatat ccgcgcgggt cgttgacgtt tgattcccat aattgacgcg
     3301 gagggccggc aaacgcgccc cacaagcgaa tcagttctgc ggttagcgaa gttccactgc
     3361 gcataacgcc taaaacaatg atgcgtttta gcgtgcggct acctttttgg ggcgggacct
     3421 tcttgttcat tttcgagagg gtgcgggcga tttgaccttt gacgaaatgc gcgaagttgt
     3481 tccggcgatt gtcgccatcg cgtgatttgt atcttccaga tgcagaccca acatacgttt
     3541 cacatcgtaa gccccactgc cgaaccgaac caatctgacg ttctgttcaa aggcctcctg
     3601 cagggcggca tacagcaaaa agaaatatcc gccgggtacg tcatcttcga gtcccagggc
     3661 tgttgtcaat tggaagcggt tatcgcgcat ggtcgcagca catccaacga actgtccatc
     3721 cttgtggatt tgaagccacg tcccgtcaat catagagaag tttgtcagca ggccgcgtat
     3781 ccaggggttg agtgcagagc cattccagat cgagacgttt tgaattagag taagcgcgga
     3841 ctcaacgtcg ggaacctctt tgtgtcgagt aagagacagt ccactagttt cagctagttt
     3901 cagattctcc ttgaaatatt gccgctcgtt cttattacgc gatttcagat aagtctcgaa
     3961 actatcccat tcaattggca tgtaggtccc tggctcagaa acggtaatgg gctcacagcc
     4021 cggtggccaa cctgggtagc gcaactgctc ggtcagcagg aaatcgaaaa ccaggaagga
     4081 acagcgttga cgcttgaatt cctgctgggc tgttttggcg agcacttcca gagctgcatc
     4141 acggaccggc tcacccggaa gcaacatggc ggaggtatca gccagggggg atcggcaaac
     4201 aagcaatggt cggcgtttga ggaccgatgc cataaatcgg cgtgcgatgc ttgggagagg
     4261 gagtggctcg ttatgaaccc tgaaaagagc cgcaccagcg attggggtat ctccatccaa
     4321 agccagcagg taggttggct ggcactctgc cataacgtgt tcgccaaacc gataccaact
     4381 atggctttga aatccccgcc ccgagacgac ccgattccag atttcgggat caatttccgt
     4441 gatgctcttg aaggtctgaa ctgctaattc tgccatgctc gctcccaaat gtttccattt
     4501 tcatgtttcg tataaaagtg tactctaaat tgcgtagctt gtataatcat accggagaac
     4561 atgttatgga aaattatacc tttcgtttaa aatctgcgca ggacttattt gactcaattg
     4621 aggcgtttgc ccgcgaacag cagattgagg caggttgtgt actgagcggg atcggcagtc
     4681 ttacccattt taccgtccgc ctggctgacc gtgaaattcc cagtgagtat gaggggcctt
     4741 ttgaaattgt ttcattggca gggacggttt ccatttatgg ttcacattta catatttcag
     4801 cgtctgacgg cgatggccgg acgatcggtg ggcatttggt accaggctcc aagatctata
     4861 cgacggcaga aatcgtgctg gcagcttttc cgggtactgt ttataagcga gagtttgcag
     4921 aggattccgg atatgatgag ctggcagttt acaagaaata gacagatggt caggttgacg
     4981 aaagctcact actccatcag gaaactactt tttcagggca gctaaaaatt cctcctgcgt
     5041 tcgcacggtt tttccaagcc ctaaccgttt gcccagccgg gtcagttggg gtggttccac
     5101 atacgtggtc gccagaattt cttcctgtcc caatacatca tttgcttttc cgtccagcaa
     5161 cacctttccc tgagaaaggg cgatgacccg ttcgaaattt tctgcacaaa aatcaatatc
     5221 gtgagtgatc gtgatgagtg tcttaccgcg ctggcgcaag gtccgaatgg cgttagcgat
     5281 gcgtgcaatg ctcatggcat cctgtccggt tgtgggttca tcgaatatga tgatgtctgt
     5341 atccattgaa atcacggatg ccaacgctac cattttacgc caggtggggg ataggtcata
     5401 cggattcatc tccattttgt ctgataattc cgtcatttca agggcgtctt tgacaagact
     5461 ctttacctgc tcaccgtcga gtttgaggtt acgcggtccg aatgcgactt ccgtttccac
     5521 attcttggag aacagttgct catccggatt ctgaaacaca taccctactc tccgcgccaa
     5581 cttcgccact ggctggacat tcgtatccca gccaccgatc cgcacgctac cttctgtggg
     5641 cgtgagcagc ccgttcaggt gtttgaccag cgtcgtcttc cccgcaccgt tttgaccgac
     5701 gatcgcaacc tgctcaccgg aagcaatatc caatgtgacg ccgcgcaacg cctgaacgcc
     5761 tgtgggataa gtaaagtgca aatctctgat ttcgattttc actttgccac gaccttcgag
     5821 aatccgtcta cagcttcatc cagtgttatt ggcaaactat cactttgcca aaggcgcatc
     5881 ttttgtgcct tgcgcgctac ggaagtgtag cgcgaaatcc caaacccgct ttcttcaacc
     5941 agtggggagg tcaatacttc atcgggtgtc ccttcgagca acacctgccc atcgtccagc
     6001 gcaatcactc gatctacgta gtgcgcgatc cattccactt tgtgctcgac taagattacc
     6061 gtcattcctc gctctgccat tttttgaatc gcgatgaaga cttcgcgggt gccgatggga
     6121 tccatttgcg atgtcggttc atccagaacc agcacttttg gttgcatcac gagaatagaa
     6181 gtcaacgcaa cgcgctgctg ctggcccccc gaaagggaat agggggaacg atttgccagt
     6241 tcactaatgc cggttaattt catggcatcc tccacgcgag ccatcatttc gtcacgtggt
     6301 attccaatat tttccaagcc gaaggcgagc tcctcaaaaa ccgtatactt cgctccactg
     6361 atttggttga acgggttttg aaatgccagc ccgacattta atacccattc gtgaagggat
     6421 gaattctgcg aatccctgcc gagcacctca acggttccgg tcatctctcc tttgaaaaaa
     6481 tggggaacaa agcctgcgat cgcatagcac agcgtggatt ttcctgcacc gttcgggccc
     6541 acaatcgcga cgaattcgcc atcctctact tttagattga tgtttttcaa aactggtgcg
     6601 tttgtcagcg gatatttgta ggtaacgttc tgaagattta caatagccat agtctcaaag
     6661 ccagtgaaat aatcacaagc gcagccagtg tccagcgcat tgttctgtct aaacttgtgt
     6721 caggaatttc atgtaaggat gttttcgcct ttgtggatgt aaacccacgt gcctcaatcg
     6781 cgatggcacg ttcctccact tccacgagag agccaaatac cagtggaccc aggagcggaa
     6841 ctaacgagcc ggcacgtttg atgaaactgc cttgcgtatc caacccacgc gaacgttggg
     6901 cagagatgat cgtctgggcc ttggcttgca tttgaggaat aatttgcagg gaagaaataa
     6961 ttacataggc gaattgaccg ggcagtccgc gtcgcgttag atcagacatt aactcactgg
     7021 gatgtgtggt taacaacata atcgtgaacg acgaaaccat cacagtgata cgacttgcgt
     7081 tccgaaaggc aagccggatg gataattctg tgatttccaa cccgacaatt tgaaagacta
     7141 cttttgattc gccgggcaaa aatagtgcct gcatgacgaa cagaaaaagg acagttggca
     7201 gtataacttt aaaaaagaaa ctccaatact ccggcgcaac ttttccgatc acagtaagtg
     7261 gaatgattac gaagatgaat attgccagcg gcgtccagta ccacggactt gtaaatgcga
     7321 ttaacacaag cgaaaatgcc aataccactt tcgtcatcgg gttcaggcgg tgaatggggc
     7381 tatcgcgttt tatgaaaaaa gaaagacgtt catgcataga tggattcttg gatttgggtt
     7441 gagcaccatt ataaaagaaa aggccagaca tttctgcctg gcccttgatt cgtggatggg
     7501 ctcaaaacta tccgccagta agtctttgaa ggaggaagat ggcgaagagg attaatagaa
     7561 cgaccaccac cactgcgata attaactggg tctgactgac cttttcgtct gtttccacgt
     7621 tttctgcacg cgggaagcgg gcaatgaaac gtttggagag tccctgaatg atcgcaaacg
     7681 ccaacatggc agtcgtgatt ttatctaccg gttcggtaag gaagctcgat gcaaatactg
     7741 cttgcacgat gcccctgccg gattccacga ggaaggctgt gatcaagccc gtgcctccgg
     7801 ttgtgatccc gccgtagacg aggagcgaaa tcggtacaga ggacatcgca gcagccaggg
     7861 caataaagaa gccagttacc acaaccttcc accaactctt gaataatcca tagttggcac
     7921 agatgcctga gaccaaacca atcacggcag ctaccggcca ccagggcagg aagtcagggt
     7981 tgagtgtgag tcccagaatc gtatttgcaa gcgcgccagt caacgcaccg gcccatggac
     8041 cacagatgac gcccaccaga acggtgccaa tagaatccag atatacgggc aatttgaggg
     8101 tagcaacgat ttgtccgaca gccatattaa tagcgattgc caccggcatg agcacccaag
     8161 tcatcgtgtt gaaatctttt ccgatattga gccaggaagt gcttttctgg ctttgcatag
     8221 ctgtttctct catggttcct ccattgattg aatagttttc cgcacggtca attcgactgc
     8281 tgaaggttga gccaggtact gctcacatga cgtgcggctc ggcttgtaaa ttgtaaccct
     8341 aaaggcttta cacaggttcg gtcacaaatg agcgtatcgg gcctagtttg agggttggcg
     8401 acggttctgg ttggactggg ccaaagtttc cattctttcg acaaacagat cgataaaatc
     8461 acgcggccga actcccaggg ccaccttcac attggcaggt ttttttccat agttataaaa
     8521 atccgccagg gttttgcccg tggagatccc acccgagata tcgacatcca ctggcaactc
     8581 ctggtaatca cataattgcg gtgcaaatgt tagagccaac gcaagtggat cattaatcac
     8641 acagccctct atccctt
//
LOCUS       DASTJP010000699         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1547188, whole genome shotgun sequence.
ACCESSION   DASTJP010000699 DASTJP010000000
VERSION     DASTJP010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547188"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFQ23_20455"
     CDS             <1..605
                     /locus_tag="VFQ23_20455"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9909032.1"
                     /translation="YLIIDETHTICTSPGGYTASFGLQPDFLTIGKPLAGGVPAAVYG
                     FTEEVSQQFSSRLAVDDADVGGIGGTLAGNALSIAAMKATLQNVLTDEFYEKSIQLQE
                     RFTAGVESVIREFDLPWIVKRLGNRSEYWFRPSPPRNGGEAFAAIDHDLDRYMHLLAL
                     NRGILLTPFHNMALISPDITEADVDYHTTVFREAVTSLFR"
     gene            complement(651..723)
                     /locus_tag="VFQ23_20460"
     tRNA            complement(651..723)
                     /locus_tag="VFQ23_20460"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(688..690),aa:Thr,seq:cgt)
     gene            complement(785..1351)
                     /locus_tag="VFQ23_20465"
     CDS             complement(785..1351)
                     /locus_tag="VFQ23_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909033.1"
                     /translation="MTPESTRLIVEFIGAIVIASMTFYFTRSHYLRKRQDDLADKLIE
                     KREKRIEEANAVVDNMAELSMLVSKQGLELTHLRNPDDYARRRSEIAERMESLSIKVA
                     CIFLIKDAKLSKYHNQFAATIAGESNKISQILVRLITEKEIINENEFTDNLKAFNKRC
                     YVLHKAMKKRLDELSQDNFKMDSFPEKT"
     gene            1770..3017
                     /locus_tag="VFQ23_20470"
     CDS             1770..3017
                     /locus_tag="VFQ23_20470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9909034.1"
                     /translation="MLRIAMISYHTCPLATLGGKDTGGMNVYVRDLTRQLGQMGIHVD
                     IFTRSQDDHVPHVLHELGYGNRVVHVPAGPEHPISKQELVDYIPEFVEGIKAFACEKR
                     IKYDIIHSHYWMSGLAAAALSDAWGGTPIVHMFHTLGEMKNRIARTEAEREGEYRING
                     EKQVLRRADRVVVATVAELTQLRFLYKANGNMVVIPPGVDVSHFYPIPSDEAKMYVGL
                     KPDDRMVLFVGRIEPLKGVDTLIEAMSCLQLKEKNRPVHLAIIGGDPAASPEEMTVEM
                     ARLQKLCDDLSVDQTVVFLGKRDQDKLPYYYSAAQLVVMPSHYESFGMVALEAMACGT
                     PVVASEVGGLAYLVRDGETGFTIPDQEPDELCDKISWLLNDRELHETMSARAVEYAQD
                     YAWEKIAKQIVEVYEGLMEIRDD"
     gene            complement(3041..3841)
                     /locus_tag="VFQ23_20475"
     CDS             complement(3041..3841)
                     /locus_tag="VFQ23_20475"
                     /inference="COORDINATES: protein motif:HMM:NF040088.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909035.1"
                     /translation="MIDKGSSSANDRNMKISSDTGIKDTGVSQLYEILIGYYNFDELW
                     DLAGKLAIDLDTLPGDNKNSKAIELLAFARRHGRLSDLANILQNTKPQFFTEDKSGLT
                     IQTSADQVTNLNLELSNLRQQLTELQNLPKDSQIGNRIEDILKIANRAVGRSEELSAR
                     IILPPQEITDVYLIPAHSLDHLEEYRSDESFIYLLIGVFSGATLGILSNWATTEPFFI
                     TRFSIILLAILLVLTLACILWAWRLHKRTIALKDRLLNHRLFSEHTDS"
     gene            complement(3923..>4430)
                     /locus_tag="VFQ23_20480"
     CDS             complement(3923..>4430)
                     /locus_tag="VFQ23_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909036.1"
                     /translation="RNSSETQLFPKRDLVQELPLSGITPENQVLALIAHDQMNLEMCC
                     FVVAHARQLFNEFDYILATDVTGEWVKKFALAAGRSDEEISKVRCCLSGSYGGYIQIA
                     AAVVKKLCRKVIFLQDPFTSHAHETDIRLFEQAVLLFERAAVHGVEIELATNIASAKA
                     ILGYRELS"
BASE COUNT         1192 a         1005 c          998 g         1235 t
ORIGIN      
        1 catacctgat tattgatgag acccacacga tttgcaccag tcccggcgga tacacggcct
       61 cattcggact ccagcccgat ttcctcacca tcggcaaacc gctggctgga ggcgtgcccg
      121 cagcggtcta cggattcaca gaagaggtga gccagcaatt ttcatccagg ctcgcggttg
      181 atgacgcgga cgtcggcggc atcggcggaa cgctcgcagg caacgcgctt tcgattgcag
      241 caatgaaagc cacacttcaa aatgtgttga cagacgagtt ctacgaaaag tccatccagt
      301 tgcaggaacg gtttaccgcc ggggttgagt cggtgatcag agaatttgat ctgccatgga
      361 ttgttaagcg actcggcaac cggtccgaat attggttccg ccccagccct cctcgaaatg
      421 gtggcgaagc atttgccgcg atcgaccacg acctggatcg ttatatgcac ttgttggcac
      481 ttaatcgagg aatcctgctg acacccttcc ataatatggc attgatttca cctgatatta
      541 cagaagcaga tgtagactat catacgacgg tattccgcga agcagtaaca tccctctttc
      601 gataataaaa atacccatcg agagacaagt ctctgatgag cattttggtg agccaccgac
      661 gagagtcgaa ctcgtgacct aacgattacg aatcgtttgc tctaccaact gagctacggt
      721 ggcgaattta ccctatatta tggcgttaat ttggtctagt ttaccatata gttatatggt
      781 tttcttaggt cttttcagga aagctatcca ttttgaaatt atcttggctt aactcatcaa
      841 gccttttttt cattgcctta tgtaaaacat aacatctttt gttgaatgct ttcaagttgt
      901 cagtgaattc attctcgtta ataatttcct tctctgttat caatctaaca agtatttggc
      961 ttattttatt actctcacca gcaatggtag cggcaaattg attatgatat ttgcttaatt
     1021 tggcatcctt tataagaaag atgcaagcta cttttattga aagagattcc attctttcgg
     1081 caatctcact acgccttctt gcataatcat caggattcct aagatgggtt agttcaagac
     1141 cttgttttga aaccaacatg gaaagttctg ccatgttatc aacaacagca tttgcttctt
     1201 ctattctctt ctcccttttc tcaataagct tatctgctaa atcatcctgt ctctttctca
     1261 ggtaatgact tctagtaaag taaaaggtca tagatgcaat tacaatagca ccaataaatt
     1321 caactatgag tcttgtgctt tctggtgtca tactacataa atccttgttt tcgttccaac
     1381 atagcgatga tacaaggatt ctacatcata aaaacgcatc acaataatta agcccaaagt
     1441 aatctgttca tgtaggctag atgctgttca taatgggcta aataacccat atgattatca
     1501 atcgtttact ctagtgggct acggtggcga ggggcttagc ggcggggatt gttgcggatt
     1561 tctacttttc acgcaagtaa tgtcattgcg agcggcgaag cgccgaagca acacctgcat
     1621 gatttgccat gaaatgctga cgcatttcaa aaatcacgtg cggcgcaagt ctctcagcga
     1681 gccttcaagc ataagattaa tcaggatacc accagcctcg caagttttcg gctttacaag
     1741 tcgtctatac cttgggaaaa acaagattta tgttacgtat tgcaatgatt tcttatcata
     1801 cttgtccgct ggcgacgctt ggcggcaagg acacgggcgg aatgaacgtc tacgtccgtg
     1861 atctgacgcg tcagttgggg cagatgggca tccatgtgga tatctttacg cgttcgcagg
     1921 atgaccatgt tccgcatgtt ttgcatgaat taggttatgg taaccgcgtc gttcacgttc
     1981 ctgctggtcc agagcatccc atttcaaaac aggagttggt ggactatatt ccagaatttg
     2041 tagaaggtat caaagccttt gcctgcgaaa agagaatcaa gtatgacatt attcacagtc
     2101 actactggat gtctggactg gcagccgccg ctctttcgga cgcctggggc gggactccga
     2161 ttgtgcacat gttccacacc ctcggcgaga tgaagaaccg catcgcgcgt acagaagcag
     2221 aacgcgaggg cgaataccga atcaatggtg agaagcaagt cctccgtcgc gcagatcggg
     2281 tggtcgtcgc aacggtggct gaactgacgc aattgcgttt cctgtataaa gccaatggaa
     2341 acatggtggt catcccgcca ggcgtggatg tgagtcactt ttatccgatt ccctcggacg
     2401 aagccaagat gtatgtaggc ttgaagcctg atgatcgaat ggtgttgttt gttggacgaa
     2461 tcgaaccgct caaaggggtg gatacgctga tcgaggcgat gtcctgttta cagttgaagg
     2521 aaaagaacag acccgtgcat ctggccataa ttggcggcga ccccgcagcc agccccgagg
     2581 aaatgacggt tgagatggcg cgcctgcaaa agttatgtga cgatctctcc gtagatcaaa
     2641 cggttgtttt cctcggtaaa cgcgatcagg ataagttacc gtattactac tccgctgcgc
     2701 aattggtggt catgccttcc cactatgaat cttttggcat ggtcgcactg gaagcgatgg
     2761 cgtgcggcac gccagtggta gcatccgagg tgggagggct ggcttatctg gttcgagatg
     2821 gcgagacggg cttcaccatt ccagaccagg agccggatga actgtgcgac aagatctcat
     2881 ggctgctgaa cgaccgcgag cttcatgaaa ccatgagcgc ccgtgccgtc gaatatgcac
     2941 aggactatgc gtgggagaag atcgcgaaac agattgtgga agtgtatgag gggttgatgg
     3001 aaatcagaga tgactaataa agcaaatatc aagcagattg ctaactgtct gtgtgctctg
     3061 aaaagaggcg atggtttaac aatcggtcct tcagtgcaat ggttctttta tgaagtcgcc
     3121 aagcccaaag aatgcaggca agcgtgagaa caagaagaat tgctaacaaa ataatagaaa
     3181 atcgggtgat aaaaaatggc tcagttgttg cccaatttga tagaattcca agggtcgcgc
     3241 ctgaaaatac tccgataagc aggtaaataa agctttcatc agaacgatac tcttcgagat
     3301 ggtcaagcga gtgggcaggt ataagatata catctgtaat ttcttgaggc ggaagaatta
     3361 tgcgagcact caattcctcg cttcttccaa ctgcgcggtt agctatctta agtatatctt
     3421 ctatgcggtt cccaatctgt gagtcctttg gtaaattctg aagttcagta agttgttgcc
     3481 taagattaga caattctaag ttcaaattag ttacctgatc ggctgatgtc tggatcgtca
     3541 agcctgattt gtcctctgta aaaaactgtg gttttgtatt ttgcaaaatg tttgccagat
     3601 cagacaacct tccgtgacgc cttgcaaaag caagtagctc tatcgctttc gaattcttgt
     3661 tatctcccgg caaagtgtct aagtcaatag caagtttacc agccaaatcc cacagttcat
     3721 cgaagttgta ataacctatc aggatttcat atagctgtga aacccctgtg tccttaattc
     3781 cggtatcgct tgaaatcttc atattcctat cattcgcaga gctcgaacct ttgtcaatca
     3841 tgatcttatc tattcaaatc taaatcgata cacaatgtat tgatttagat ttcatgcatt
     3901 aagatataaa cttacgaatc aatcagctta attctctata acccagaatc gctttggcag
     3961 atgcgatgtt tgtagcaagt tcaatttcca ccccatgtac agccgccctt tcaaacaata
     4021 acactgcttg ctcaaacaat ctgatgtcag tttcatgtgc atggcttgta aatggatctt
     4081 gaagaaatat gaccttacgg caaagtttct tcaccacggc agccgcaatt tgaatatatc
     4141 caccatatga tccggataga caacatctga ccttgcttat ttcctcatca ctacgaccag
     4201 cagctagtgc aaacttcttg acccattctc ctgttacatc agtcgcaaga atgtaatcaa
     4261 actcattaaa gagttgccgt gcgtgtgcaa ccacgaaaca gcacatctca agattcattt
     4321 ggtcgtgagc aattagagcc aagacctgat tttctggtgt tattccactc aagggcaact
     4381 cttgaaccag atcccttttt ggaaaaagct gagtttctga tgagtttctc
//
LOCUS       DASTJP010000700         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_588248, whole genome shotgun sequence.
ACCESSION   DASTJP010000700 DASTJP010000000
VERSION     DASTJP010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588248"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /gene="recR"
                     /locus_tag="VFQ23_20485"
     CDS             <1..609
                     /gene="recR"
                     /locus_tag="VFQ23_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET9909037.1"
                     /translation="PMLLPESLQSLINALERLPGIGPKSASRLAFFFLRAPEEVSQDL
                     ASALANLKANTTLCQECFNITEAGREQCEICESSARDSSLVCVVEEALDVLALERTGG
                     YRGKYHVLQGVLSPIEGIGPDDLKIKQLIARVGRGEIKEVILATNPSMEGDATALYLQ
                     QHLEPLGVRVTRLARGLPVGGDLEYADQNTLLRALSGRQEMN"
     gene            complement(640..1476)
                     /locus_tag="VFQ23_20490"
     CDS             complement(640..1476)
                     /locus_tag="VFQ23_20490"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HET9909038.1"
                     /translation="MTKIGILHPGEMGISIAASAINSGHEVYWLSEGRSDKTRARAER
                     HNLKELESFVAFCQTCEVIISICPPHAAEEVARTVSEAQFQGLYLDANAISPQRAAKI
                     GQMLETSGIQFVDGGIIGGPAWKPKETWLYVSGDHANEITSCFSSGPLETKIIGDEIG
                     KASSLKMCYAAYTKGTTALLSAVLAAAESLGVRDELYQQWNMDEQDFSKQVNRRVTRV
                     TAKAWRFEGEMKEIASTFQESGLPNGFHLAAAEIYHRMANFKDTSESPPLEDVLKTLF
                     SH"
     gene            1530..2786
                     /locus_tag="VFQ23_20495"
     CDS             1530..2786
                     /locus_tag="VFQ23_20495"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9909039.1"
                     /translation="MRITILTYGSRGDVQPFLPLSRRLMEEGHSVKLAAPSRFNKFVE
                     EHGIGFVPLPGDPEDLSRRLNNAGRNFVKLLRELTEHAVEIGADVWRQTEEACQDADL
                     ILHTFTHAVGAHTLAREKNIPDVHIQTFPMFTPTGDYPNVTLPDLRSHTLNRLTHNLS
                     AQITWWTSRIGFERVRRREGFAHRKLYWPFDHDSLHPRTPILCAWSPRVLPPSSDWPP
                     NVHVTGYYFFADHGSYQPHQELESFLRWGKPPVCVSFGSMVNRDAKKNDGIVRTALEQ
                     TGNRGIILSGWSGVKSSSSNDLLYLDAAPHDWLLPRCKLVVHHGGAGTTAAGLRAGIP
                     NIVVPFMADQPFWGRRVHAIGVGPQPLRVKSLSVKGLVRAIAEAEDQSLRTRAQSIGQ
                     QIRNEDGVGIAVELIEKYFNNFQRKY"
     gene            complement(3127..>3316)
                     /locus_tag="VFQ23_20500"
     CDS             complement(3127..>3316)
                     /locus_tag="VFQ23_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909040.1"
                     /translation="TGFDRVVASYHYSVVKVLVTKRAGFYTSRLGLSRHQQTKTPMSF
                     VFRHRLVRLQNPQACVSD"
BASE COUNT          812 a          819 c          831 g          854 t
ORIGIN      
        1 cctatgcttt tacctgaatc tttacaatca ttgatcaatg ccttggaacg cctgccggga
       61 atcgggccta aatcggcgtc gaggctcgcg ttcttcttcc tgcgcgcacc tgaagaagtc
      121 tctcaggatt tagcttctgc gcttgcgaac ctgaaagcca atactacgct ttgtcaggaa
      181 tgctttaata ttacggaagc cggccgggag caatgtgaga tctgcgagtc atccgcccga
      241 gacagcagtc tggtctgcgt cgttgaagaa gccctggatg ttctggcgct ggagcgtacc
      301 ggcggctatc gaggtaaata tcatgtcttg cagggagtct tatcgcccat cgaaggcatc
      361 gggccagacg acttgaagat caaacaactg atcgcacgtg ttggacgagg tgagatcaaa
      421 gaagttattt tggcgaccaa cccgagcatg gaaggcgacg cgacggcact ctacttgcag
      481 cagcatcttg agccgctggg tgtaagagtt acacgccttg cgcgcggcct tcccgttggc
      541 ggtgatctgg aatatgcaga tcaaaacacg ttgttgagag ccttatctgg tagacaggaa
      601 atgaattaaa aacaatcgcc cccaccaggg cgattgtttt taatgtgaga acagtgtctt
      661 caagacatcc tcaagcggcg gactttcact tgtatctttg aagtttgcca tgcgatgata
      721 gatctctgcc gcagccaggt gaaatccatt gggaagacca gattcctgaa atgtcgatgc
      781 gatttctttc atctccccct caaaccgcca tgcctttgcc gtgacacgcg tgactcttcg
      841 attgacttgt tttgaaaagt cctgttcatc catattccat tgctgataaa gctcatcgcg
      901 cacgccgagc gattctgccg ctgcaagaac tgcagagagc agggcagtgg tcccttttgt
      961 atacgccgcg tagcacattt tcagcgacga ggcctttcca atttcatctc caatgatttt
     1021 tgtttccagc ggtccgctgg aaaagcagga tgtaatctca ttggcatggt cacccgatac
     1081 ataaagccag gtttctttcg gcttccaggc gggtccgccg ataatgccgc catccacaaa
     1141 ttggattccg ctcgtttcca gcatttgccc gattttagct gcacgttgag gcgaaatcgc
     1201 atttgcatcc aggtataaac cttgaaactg cgcctcgctg acagtcctgg caacctcttc
     1261 tgccgcatgt ggaggacaga tgctgatgat cacttcacag gtctggcaaa acgcaacgaa
     1321 tgactcgagc tctttgaggt tgtgcctttc agcccgcgcc cgggttttgt cgctgcgacc
     1381 ttctgacaac cagtacactt catgcccgga attgatggca gaagccgcga tggaaatacc
     1441 catttcaccc ggatgaagga tgccgatttt tgtcatggtt tctccttatc tttgccagta
     1501 tagcatgacc ataaaggtaa aatatcggca tgcgaatcac catcctcacc tatggctcgc
     1561 gtggtgacgt ccagccgttt cttcccttgt cgcgcaggct catggaagag gggcattccg
     1621 tcaaattggc cgcaccgtct cgcttcaaca agtttgttga ggaacatggg attgggtttg
     1681 tacctctgcc tggtgatccg gaggatttga gtcgccgtct gaataatgcc gggcgtaatt
     1741 ttgtcaagct actccgtgaa ctgacggagc atgccgttga gatcggtgct gatgtctggc
     1801 gtcaaacgga agaagcctgt caagatgctg acctgattct tcacacgttt actcatgccg
     1861 tcggcgcaca cacactcgcg cgcgagaaga atatccctga tgtccatatt caaacatttc
     1921 caatgttcac accgacaggt gattacccaa atgtcacctt gccagatttg cgatcccaca
     1981 ccttgaatcg cctgacacat aatctctccg cacagatcac atggtggacg tctcgcattg
     2041 gatttgaacg tgtgcgccgc cgtgaaggat tcgctcatcg aaaactttat tggccctttg
     2101 atcatgactc tctccacccc cggactccga tcctgtgcgc ctggagccca agggtgcttc
     2161 ctccctcaag cgactggccg ccgaatgtgc atgtcacggg ttattacttc ttcgctgatc
     2221 acggatctta tcagccccat caggaacttg agtcatttct cagatggggg aagccacctg
     2281 tgtgcgtttc ctttggaagc atggtaaatc gtgatgcgaa aaaaaatgat ggcatcgtgc
     2341 ggacagccct ggagcagaca ggcaatcgcg gaatcattct ttcgggatgg agcggagtta
     2401 aaagctcatc ctcaaacgat ttgctttatc tggatgcggc gccgcatgac tggttgttgc
     2461 cgcgctgtaa actcgtggtt caccacggtg gggcaggcac gactgccgca gggttacgtg
     2521 caggtattcc aaatattgtc gtgccgttca tggcagatca accgttctgg gggaggagag
     2581 ttcatgccat tggcgttggc ccccaacctc tccgtgtaaa aagtctttcg gtgaaaggtt
     2641 tggtgcgagc aatagcagaa gctgaggatc aatctctgcg tacgcgcgca cagtcaattg
     2701 gtcagcagat acgaaacgaa gatggggtgg gtattgctgt ggaattgatt gaaaaatatt
     2761 ttaataattt tcagagaaag tattgactgt catctaacag atggtgtgat attatctcgc
     2821 cgtccttttg ggtgaatgac cgatatgcgt gaaaacgaaa acgtgatttt ttaaggtgaa
     2881 atgtctgata atccttgaca aagagaacaa agcgcatata ataccgtccg ctagtatgtt
     2941 ctaaaaagaa cagagcgaat gtggaggtca gttcatgacc gatatgcata aaaacgaaaa
     3001 cgcgattttt taaggtgaat tgtctgataa ctcttgacaa aatcatcgaa atgcatataa
     3061 taaacattcg ccagtacatt ctagagatct cgtccccctg acagatcatt gaaaactgag
     3121 aagtctctag tcagagacac aagcctgtgg gttttgcagt ctgacgagcc gatgtcgaaa
     3181 gacaaaagac atcggtgttt tcgtctgttg atgtcttgac aggccaaggc ggctcgtgta
     3241 aaatccagcc cgctttgtta ccagaacttt aacaactgaa tagtgatagg aagcaacaac
     3301 tctgtcgaat ccagta
//
LOCUS       DASTJP010000701         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_694139, whole genome shotgun sequence.
ACCESSION   DASTJP010000701 DASTJP010000000
VERSION     DASTJP010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694139"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..985)
                     /locus_tag="VFQ23_20505"
     CDS             complement(17..985)
                     /locus_tag="VFQ23_20505"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET9909041.1"
                     /translation="MTKRILIDADPGIDDACAILLALASPEISLEGVSIVHGNCSLEQ
                     ATINGLSILELGNASHIPLARGCELPLVQPSLLAPETHGNTGLGYANLPIPQSKPIRQ
                     HGCDFLIERILASPGEISLVAIGPLTNVALAIRQEPRIVDSLKELIIMGGAIRSEGNT
                     TALAEFNTYVDPHAAHIVFHAGIPTTLVPLDVTYQCILTEQDVARLQVLDSPITTFIK
                     DATRFYMEYHDDYQGIEGCVINDPLALALTFAPQLCDYQELPVDVDISGGISTGKTLA
                     DFYNYGKKPANMKVALRVRPRDFIDLFIERIETLAQSNQIRRQPSN"
     gene            complement(987..1937)
                     /locus_tag="VFQ23_20510"
     CDS             complement(987..1937)
                     /locus_tag="VFQ23_20510"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET9909042.1"
                     /translation="MTPQRIIIDTDPGIDDALAFLLALASPEIKIEALTTTQGNVTLE
                     KATRNALSILELAHASHIPVVKGSVLPLVQPLRASPDVHGQSGIGNSILPEPRVKSRP
                     GHAVDYLIETILAEPNEISIFPIGPLTNIAMAIRKERRFAKAVKELVIMGGAIQDGGN
                     MTPLAEFNIYVDPHAAHIVFHSGIPITLIPLDVTQKCLLKQEHVDRLLKINSPISRFV
                     SDAMSVYLESSYARGFDGSALHDPLTLATILAPELLTLKDYYVDVDISGGVSMGNTFA
                     DIYHVAKKPANMKVAMDVRYYEFIELFVQRLEGLSRSIPD"
     gene            complement(1983..2900)
                     /gene="rbsK"
                     /locus_tag="VFQ23_20515"
     CDS             complement(1983..2900)
                     /gene="rbsK"
                     /locus_tag="VFQ23_20515"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HET9909043.1"
                     /translation="MPKILVVGSLNADLVVRAPRFPQPGETISGEDLQVIPGGKGSNQ
                     AVAAARHGVDVSMLGRLGTDNFGDYLLENLKSNKVDAQLVQRDDASTGTAIIIVDANG
                     QNSIVLSAGANGKVNEADVNAASFRDFDLLLLQLEIPIPTVLSAAERAHENGLRVILN
                     PAPAQQLPEELIALADFLIPNETELSLLTGLPVNDLTSTEQAARTLLKRGVKNVIVTL
                     GSKGALIVTNQQVTQVDTFKVDVVDTTAAGDAFIGGFATALLNGKSLEEAVRYGCACG
                     ALATTKFGAQPSIPTTEEVENFLVTQQSG"
     gene            2963..>3822
                     /locus_tag="VFQ23_20520"
     CDS             2963..>3822
                     /locus_tag="VFQ23_20520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9909044.1"
                     /translation="MKRIWPFSFYFLYFAAFASLLPFLVLFYQQLGFSGAQIGLLTGV
                     PPLITLAGAPFLTRLADATQKHKLIMGVGLTGALIVALILPLLQSFILVFLLVIAFNI
                     FMSSLTPLADSAIMTMLGAERSMYGRVRLGGTIGWGLFAPIAGWMIDTYGLKTGFWAF
                     AAIMLINLYVSQRFTFGKPHEGESNNGGIRTLLTNRNWILFLLTAFLGGLGSMSAAAY
                     LFPYMAELGASETMMGIAITISTLTELPIFFFGHHLVKRFTSRGLLLSALLLLGIRSL
                     LYALVNSTPLV"
BASE COUNT          890 a          934 c          962 g         1036 t
ORIGIN      
        1 aatgagcgta ttgggcctag tttgagggtt ggcgacggat ctggttggac tgggccaacg
       61 tctctattct ttcgataaac agatcgataa aatcacgcgg ccgaacacgc agggcaacct
      121 tcatattggc aggctttttt ccatagttat aaaagtctgc cagggtcttg cccgtggata
      181 tcccacccga gatatcgaca tccactggca attcctggta atcacataat tgcggtgcaa
      241 atgtcagagc caatgcaagt ggatcattaa tgacacaacc ctcgatgcct tgataatcgt
      301 catgatattc catataaaac ctggtcgcgt ctttgatgaa tgtagtaatt ggcgagtcca
      361 ggacctgtaa gcgcgctaca tcctgttccg tcaaaatgca ttgataggtc acatctaatg
      421 gaacgagggt ggtgggaata cctgcatgaa aaacaatgtg tgccgcatgc gggtcaacgt
      481 atgtattgaa ttcagccagc gccgttgtat tgccctcaga tcggatcgcc cctcccatga
      541 tgatcagttc cttgagggaa tcgacaatgc gcggttcctg gcggattgcc agcgcgacat
      601 tcgtcaatgg accgattgcc accaggctga tttctcccgg cgaggcaaga atcctctcga
      661 taagaaaatc acatccatgc tgacggatcg gtttgctttg agggattgga agattcgcgt
      721 accccaggcc tgtgtttcca tgtgtttccg gagcgagcag ggaaggctga acaaggggga
      781 gttcacagcc tcgagccagc gggatgtgac tggcgttgcc caactccaaa atagataatc
      841 cgttgatggt agcctgttcc agggaacagt tgccatgcac aatactgact ccctccaggg
      901 aaatttcagg cgaggctaac gccagtagaa tcgcacaggc atcatcaata ccagggtctg
      961 catcaataag aattcgtttt gtcatattaa tccggaatgg aacggctcaa accttccagg
     1021 cgctgtacga atagttcgat gaactcataa tagcgcacat ccatcgcgac tttcatgttt
     1081 gccggttttt ttgccacatg ataaatgtca gcgaacgtat tgcccatcga aacaccgcct
     1141 gagatatcca catctacata ataatccttg agggtcaata actctggcgc aaggatagtc
     1201 gccagagtta agggatcgtg taacgcgctc ccgtcgaagc ctctggcata cgaagattct
     1261 aaataaacac tcattgcgtc actgacaaat ctggaaatag gtgaattgat cttgagcagg
     1321 cgatcaacat gttcctgttt gagcaggcat ttctgggtca cgtccaatgg aattagtgtg
     1381 atgggaatac cagaatggaa aacgatatgc gcggcatgtg ggtcaacata gatattgaac
     1441 tccgcgagcg gtgtcatatt tccaccatct tgaatcgcac cgcccatgat taccaattcc
     1501 ttgaccgctt tcgcaaacct gcgttctttc cgtatcgcca tggcaatatt cgttaagggt
     1561 ccaatgggaa agatgctgat ttcgttcggt tctgcaagga tcgtttcgat caaataatca
     1621 acggcgtgtc cgggacgaga tttcacgcgt ggttcaggca atattgaatt tccgataccg
     1681 ctttgcccat gcacgtctgg cgaagcgcgt aatggctgga caaggggcag cacacttccc
     1741 ttcacgacag gaatgtgact tgcgtgtgcc aattccaaaa tcgaaagcgc gttgcgcgtt
     1801 gccttttcca gcgtgacatt gccttgtgtg gtggttaaag cctcgatctt aatttctggt
     1861 gaagcgaggg caagtaggaa tgcgagcgcg tcgtctatac cgggatcagt gtcaatgatg
     1921 attcgttggg gagtcatggc aaaaatggga tggacatagc aaatagcgga ccgttttcac
     1981 aattatccgg attgctgtgt caccaggaaa ttctccactt cttctgttgt cgggatggaa
     2041 ggctgcgcgc cgaatttcgt tgtggcgagt gctccgcagg cacacccata tcggacagcc
     2101 tcttcaaggg atttaccatt caatagcgcc gtcgcaaagc caccaatgaa ggcgtctcct
     2161 gcagcggtgg tatccacaac atccactttg aatgtgtcga cttgtgttac ctgctgattc
     2221 gtcacgatca acgcgccctt acttcctaag gtgacgatca cgttcttaac gcctcttttc
     2281 agtaaggtgc gcgcggcttg ttctgtggag gttaggtcat tgacaggcaa gccagtcagt
     2341 aggcttaatt ctgtttcatt cgggatgaga aaatctgcga gcgcgatcaa ttcttcggga
     2401 agctgctgag caggtgcagg attcaaaatt acgcgcagac cgttttcatg agcgcgttcg
     2461 gctgcagata aaactgtggg tatgggaatc tctaattgga gaagaagcag gtcgaagtcc
     2521 cgaaaggacg cagcattcac atccgcctcg tttaccttcc catttgctcc tgcagaaagc
     2581 acgatgctgt tttgtccatt tgcgtccaca ataatgatgg ctgtgcctgt ggaggcatca
     2641 tcgcgttgaa caagctgtgc atctactttg tttgatttaa gattctcaag gaggtaatca
     2701 ccgaaattat ctgttcccag gcgccccagc atggatacat ctactccatg cctggccgcg
     2761 gctacagcct gatttgatcc ctttccaccc gggatcacct gcaggtcctc gccgctgatc
     2821 gtctcaccgg gttgcggaaa acgaggcgcg cgcacgacca gatcggcatt gagcgagcct
     2881 acgaccagga ttttcggcat tcacaaattt tactcgattt gaatcttgct ttagatataa
     2941 tgggaaggtt tggaggtact caatgaaacg aatctggccg tttagctttt actttcttta
     3001 ttttgcagca tttgcatccc ttttgccttt cctggtgctc ttctatcagc aactgggatt
     3061 tagtggtgcg cagatcggac tcctgactgg cgtgccacca ctcattacgc tagccggcgc
     3121 gccgtttctt acccgtctgg ccgatgccac acagaagcac aagttgatta tgggtgtagg
     3181 gctcaccggt gcattgatcg tagcattgat tttgccgctc cttcagtcat ttatccttgt
     3241 tttccttttg gtgattgctt ttaatatttt tatgtcgtcc ttaactcctc tcgccgatag
     3301 cgcgatcatg actatgcttg gggcggagag gagcatgtat ggtcgcgtcc gcctgggagg
     3361 caccattggc tggggattat tcgcgccgat tgccggctgg atgatcgaca cctacgggct
     3421 gaaaactggg ttttgggcat ttgcagccat tatgctgatc aatctgtatg tgagccaaag
     3481 atttacattt ggcaagcccc atgaaggcga gtcgaataac ggtggtattc gaacccttct
     3541 cacgaatcgg aactggatcc tttttcttct tacagccttt ctcggcggac tgggttcaat
     3601 gtctgctgcc gcctatttat tcccttatat ggcggagctc ggtgccagcg agacgatgat
     3661 gggtattgca atcactattt ccaccttgac cgaattgcct atcttcttct tcggtcacca
     3721 cctggttaag aggtttactt cgcgtggatt gcttctcagt gccttattgc tgcttggtat
     3781 tcgctctctg ctgtatgcac tagtaaattc gacaccctta gt
//
LOCUS       DASTJP010000702         3788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1548539, whole genome shotgun sequence.
ACCESSION   DASTJP010000702 DASTJP010000000
VERSION     DASTJP010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548539"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFQ23_20525"
     CDS             complement(<1..993)
                     /locus_tag="VFQ23_20525"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909045.1"
                     /translation="MHKSPFWISDRLALFPDSTRIQNDSLFIAGHALESLAEKYGTPL
                     YVYDRTTMDASVAAYKGALKEYYPALSDITYAGKAFLCKAIAEWTQAHNLWLDCTGEG
                     EIAIAVAGNVSREHILVHGVNKSHKDLESAIRFAGTIVIDNLMELARLKEILSSLHSP
                     CPGLWLRLLPGVSVDTHHSHTQTGQHESKFGMTREEVLEATAFCKEHDLPLNGVHFHQ
                     GSNFREVSPLVNAIEIGLELAKNIGLNGEWHFSPGGGWGVAYHEDELPQPDVDEYVRI
                     VSDAVIQGCKAYGLDLPHLHLEPGRSLVARAGVAIYRVGAIKRRGEKKWILTDGG"
     gene            complement(1009..>3788)
                     /locus_tag="VFQ23_20530"
     CDS             complement(1009..>3788)
                     /locus_tag="VFQ23_20530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9909046.1"
                     /translation="GRTLVDPRWLAIRKDGSDFPPEEYATAVTLRTGHPQKGVIMGVH
                     KPDGSLTWISTNTQPLFQNDPSHASAVVATFTDITREHNLLEDEKRHTRQMRLLNEII
                     NTALETSDFEQMLQIFADRLGDLLTADGAYVSIWDAANRRILSAAVYGDLLNMQPIPD
                     LQPGEMTLTEAVLQAERVLVIEDTLNTPHLSPRLASSIPARSVLALPLIVRDLKLGAV
                     LIAFNQPHIYSAQEIFICEQAARQVALAVSKAQVFEAERRRASQLSLLEEASRRIEGA
                     LDQQAICQRTVEILVKRFGYAEAAIMLSTENNELKLIAINGTETLRLRPGFVQKMDEG
                     IIGHVATIRDVYIAKDLAYDPYYFHPTKPRSGSAISLPMLHEDQLLGVLYVESQVTNH
                     FDADDIQTLQTLTAHVTTGVQKARLYEAERRRALQLTLLEEASRQIAGLLDEREICQH
                     VVQTVVRSFGYAEASILMAVEKDELELIAISGTEDMGFRAGFRAKVGRGIVGHVALTQ
                     ETYITSDISRDAYYATPPAGPRTGSAMCMPMLYEDELLGVLYIESVILNDFDTDIAHT
                     FQTLTSHTVTAIQRARLFGISHHRLKAITAVQSVSQTVASSLELEQIFQTVVDMLKNT
                     FGYTFVSIYLLEGEVLQLGAQIGYPLAMVYTEIPVAFGIAGRAVQSRQIQFIPDVRNE
                     PGFLRASLEVESEICVPLLKEGGVLGVLNVESQQGRPLTQEDVELLASLAGPIAVSID
                     NARLHSNVKSLALTDSLTTLANRRAFDYALESEVARVMRYGPPLSLIILDIDSFKVYN
                     DTWGHLAGDGRLRAVADLLRANVRHPDIAARYGGEEFALLLPFTDKAGALLLAERLRF
                     AAQAIAPHAPQNGTAIAGYTLSLGVASFPEDGETAEEILLAADNAELAAKRLGKNRVY
                     AANSSHKLPNA"
BASE COUNT          921 a          964 c          966 g          937 t
ORIGIN      
        1 cccgccatcg gtgagaatcc atttcttttc accacgccgt ttgattgcac caacgcgata
       61 aatggctacg cccgcccgcg caaccagact tcgacccggc tccagatgta agtgggggag
      121 atcaagcccg tacgccttac aaccttggat cacggcatcc gaaacaattc tcacatattc
      181 atccacatct ggctgtggga gttcatcttc atgatatgcc acgccccatc cgccacccgg
      241 actgaaatgc cattccccgt tgagtccgat attttttgcg agttcaagac cgatctcgat
      301 cgcgttgacc agtgggctta cctcacggaa attcgaacct tgatgaaagt gaactccgtt
      361 caacggtaag tcatgttctt tgcaaaaggc cgtcgcttca agaacctcct cccgcgtcat
      421 tccgaatttg ctttcatgtt gacccgtctg ggtatgggag tggtgggtat ccacagagac
      481 gccggggagg aggcgaagcc aaagacctgg gcatggagaa tgcagactcg acaatatttc
      541 cttgagtcga gcaagctcca ttagattatc aatcacaatc gttcctgcaa atctaatcgc
      601 agattccaaa tccttatgag acttgtttac cccgtgcaca aggatgtgtt cacgtgaaac
      661 gtttccggcc actgcaattg cgatttcgcc ttctcctgtg cagtccagcc ataagttatg
      721 cgcctgcgtc cattcggcaa tggctttaca gagaaaggcc tttccggcat aggtgatgtc
      781 tgataaagca gggtagtatt ctttcaacgc gcccttgtat gccgcgacgg atgcgtccat
      841 agtggtgcgg tcataaacat ataaaggcgt tccatatttt tccgcaagag attcgagagc
      901 gtgtccggcg atgaatagag aatcgttttg aatgcgagtc gaatcgggaa aaagtgccag
      961 cctgtcggat atccagaagg gtgacttgtg catagagaga atgtaatcct aggcgttagg
     1021 gagtttgtgt gaggaatttg ccgcgtaaac gcgattcttg cccagtcgct tggctgccag
     1081 ttcagcgttg tcggccgcga gcaaaatctc ttccgcggtt tccccatcct ccgggaaaga
     1141 ggccacaccc aggctgagtg tatagcctgc tatcgcagtc ccattttgag gtgcatgggg
     1201 tgcaatggct tgggcggcaa agcgtaatcg ctcggcaagc agcaacgcac cagctttatc
     1261 ggtgaacggg agcagcaatg caaattcctc ccccccataa cgagctgcaa tatccgggtg
     1321 acgcacattg gcacgcagca ggtctgccac ggcacgcagc cgaccatctc ctgccaagtg
     1381 tccccaggtg tcgttgtaaa ctttgaatga atcaatatca aggatgatca acgagagagg
     1441 cggaccgtaa cgcatcacgc gggcgacctc tgattcgaga gcataatcaa atgcgcggcg
     1501 gttagctaag gtggtgaggg agtctgtcag cgcgagcgat ttcacattac tgtgtagtcg
     1561 agcgttatcg attgacaccg caatcgggcc tgcaagcgat gccaggagct caacatcctc
     1621 ttgtgtaagc ggacgccctt gttgagactc gacgttcaga acgcccagca ctcctccttc
     1681 cttaagcagg ggaacgcaaa tctcgctttc cacttccagt gaagcgcgca ggaagccagg
     1741 ctcattccga acgtccggaa tgaattggat ttgtctgctc tgcacggcac gccctgcaat
     1801 tccaaaggca accgggattt ctgtgtacac cattgccaat ggatagccga tctgtgcacc
     1861 caactgcagt acttctcctt ccagaagata aatgctgacg aaggtgtagc cgaatgtgtt
     1921 tttcagcata tcgactacag tctgaaaaat ttgttcaagt tccaatgaag aggcaacggt
     1981 ttgcgatact gactgaaccg ccgtgattgc ttttagccga tggtgagaaa taccaaaaag
     2041 acgtgccctt tgaatcgcag taacggtatg gcttgtaagg gtttgaaagg tatgcgcgat
     2101 atctgtgtcg aagtcattca ggatcacgct ttcaatatat aaaacgccaa gtaattcatc
     2161 ttcatacagc atgggcatgc acatggcgga tccagttcta ggtccagcgg gtggtgtagc
     2221 atagtatgca tccctggaaa tatcgctggt tatataagtt tcctgggtga gagctacatg
     2281 cccaacaatc cctcggccaa ccttggcgcg gaagcctgct cgaaagccca tatcctccgt
     2341 cccgctaatg gcgatcaatt ccaattcatc tttttcaact gccattaaga tggatgcttc
     2401 tgcatagcca aaacttcgca ccacagtttg cacgacatgt tgacagatct cgcgttcatc
     2461 caataagcca gcgatttgac ggctggcttc ttcaagcaaa gtaagttgta aggcgcgacg
     2521 ccgttctgct tcataaagcc tggccttttg tactcctgtg gtcacatggg ccgttaaagt
     2581 ttgcagggtt tgtatatcat ctgcgtcgaa gtggtttgtg acctgacttt ccacgtaaag
     2641 aactcccaac agttgatctt catgtaacat cggcaggctg atggcggaac cggagcgagg
     2701 cttggttggg tgaaaataat atgggtcgta ggccaggtct tttgcaatat aaacatcgcg
     2761 aattgtggcc acatggccaa taatgccttc atccattttc tgcacgaacc ctggtctgag
     2821 gcgcagcgtt tctgttccat taatagctat cagtttcagt tcattatttt cagtggaaag
     2881 cataatggcg gcttccgcat aaccaaagcg cttcaccaaa atttccactg tacgctgaca
     2941 aatggcctgt tgatccagcg cgccttcaat acgacggctg gcttcttcga gaagcgacag
     3001 ttgcgaagcc cgacgtcgct cagcctcgaa tacctgagcc ttggagaccg ccaacgcgac
     3061 ctggcgcgca gcctgttcgc agatgaaaat ttcctgtgcc gaatatatat ggggctgatt
     3121 gaaggcgatc agcacggcac ctaatttaag gtctcgaacg atcaacggaa gggcgagaac
     3181 agatcgcgca ggaattgacg aagccaaacg cggactgaga tgtggagtat tgagtgtatc
     3241 ctcaatgaca agcacgcgtt cagcctgcag gaccgcttcg gttagtgtca tttctccagg
     3301 ctgaaggtca gggattggct gcatgttcag gagatcgcca taaactgcgg cagataggat
     3361 acgtcgattt gcagcatccc agatggaaac atatgctcca tctgctgtca gtaaatcacc
     3421 cagccgatca gcaaatatct ggagcatctg ttcgaagtcg gaggtttcga gtgctgtatt
     3481 gataatttcg ttcagcaatc tcatctgccg ggtatgtcgt ttttcatcct cgagcagatt
     3541 atgttcgcga gtgatatctg tgaaggtcgc tacaactgca gatgcgtgtg acgggtcatt
     3601 ttgaaataat ggctgggtgt tcgtcgaaat ccaggtcagg cttccatcgg gtttatgaac
     3661 gcccatgatc accccttttt gagggtgacc ggtacgcaag gtgacagccg tcgcatactc
     3721 ctccggagga aagtctgaac cgtctttccg aatcgcgagc caacgtgggt caactaaggt
     3781 tcgtccct
//
LOCUS       DASTJP010000703         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1222927, whole genome shotgun sequence.
ACCESSION   DASTJP010000703 DASTJP010000000
VERSION     DASTJP010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222927"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFQ23_20535"
     CDS             complement(<1..849)
                     /locus_tag="VFQ23_20535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909047.1"
                     /translation="MNTPEVPIPDFFDPNKVGTVWRIPYEERAKQARDWARQHGIGPA
                     SADSTKTWLMLIDVQNTFCIPDFELYVGGRSGRGAVEDNGRLCEFIYRNLGNITQITA
                     TMDTHMTMQVFHAIFFVDQNGNHPAPYTDIHAVELRDGKWTFNPALAPQFGIAPEYGQ
                     QMMLHYAEELEKKGKFALTVWPYHAMLGGIGHALVSSVEEALFVHSIARNAQYEIEIK
                     GDKPFTENYSAIGPEVLTGPIGETLGTHNQKFIQQLQQFDRLIIAGQAKSHCVAWTVS
                     DLLEDIA"
     gene            972..1550
                     /locus_tag="VFQ23_20540"
     CDS             972..1550
                     /locus_tag="VFQ23_20540"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9909048.1"
                     /translation="MASHFQLIMRSGPTPGAVFNLGGEQLTVGRDSTNDVVINDAEIS
                     RRHARLTFQGGKYILEDLGSTNGTFVNGQRLAGPRVLKAGEVVSFGEQIVLVYEASTF
                     DPAATIASPRATAVPSASRPISAPPPAPADYAGSIPASPVVPPSAPARTNLTPILIGV
                     GFLLFLCTCAGILWFIDANFLWCTFFPFLGGC"
     gene            1855..3093
                     /locus_tag="VFQ23_20545"
     CDS             1855..3093
                     /locus_tag="VFQ23_20545"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9909049.1"
                     /translation="MNDITSTSPRWSPTTKLLVGLVVVGIVAFLLYRFSNLITPLLMI
                     FILAYLFHPLASGISNSLQISWRAAVNLLYLLIVILFIGLVTLGGVGLVQQVQSLIGV
                     IQDILANLPEYVENLSGQVFQIGPFRLDMRTIDLNALSQQVLSFVQPLLGRTGTLVGA
                     LASGAAEALGWTFFVLLVSYFLMAESSGLQSNLLRVDVPGYSRDLRTIGGKLSRIWNA
                     FLRGQIFIFLLSTVIYIILLSLLGVRYALGLALMAGLAKFLPYIGPAITWVVMGLVTF
                     FQPGKPFDLSSLAYTAIVVIITLVIDQIIDSFITPRIMARTLKVHPAAVLVTALVAAN
                     LLGLLGVVIAAPFLASVTLLGRYTMRKMFDLEPFPERETELPSPVDSEWIRQIRKLWS
                     ATLQRRKRELIESKRGDRNE"
     gene            3086..3268
                     /locus_tag="VFQ23_20550"
     CDS             3086..3268
                     /locus_tag="VFQ23_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909050.1"
                     /translation="MSNNHTEPKTETIAETDNFLAWKAEEPDGETTYHIELNNVTLHF
                     FQEEWNEFLQLVRDLG"
     gene            3370..>4341
                     /locus_tag="VFQ23_20555"
     CDS             3370..>4341
                     /locus_tag="VFQ23_20555"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET9909051.1"
                     /translation="MKLKNAITDVRGIEVGHAQDEQALTGCTVILCRKGAVAGVDVRG
                     GAPGTRETDLLNPINLVGQVHAIVLAGGSAFGLDAASGVMRFLEENKIGFNTGAARVP
                     IVPSAILYDLNLGRADVRPDSAMGYRAAAAASALAPQEGNAGAGMGASVGKMFGSKLA
                     MKAGLGTASLAIGGGIVVGAIVAVNAFGDVIDPHTGSIIAGLRSGKVGPVRIGKKDHF
                     ADTLAAMKNPVGRGVLSLASHTNTVIGVVATNAKLTKAQATKVAQMAQDGLARAIRPA
                     HTMLDGDTIFALSTGNRKADISTIGAFAAEVMAQAIVRGVKMAVSAGG"
BASE COUNT          969 a         1087 c         1148 g         1137 t
ORIGIN      
        1 ggcgatatct tcgagcaggt ccgaaacagt ccacgcgaca cagtgacttt tcgcctgccc
       61 ggcaatgatc aggcgatcaa attgctgcaa ttgttgaata aatttttgat tatgtgttcc
      121 cagtgtctca ccgatgggac cggtcaacac ttcagggcca atggcagaat aattttcagt
      181 aaagggttta tcgcccttga tctcaatttc atattgggcg ttgcgtgcaa tagagtgcac
      241 gaataatgct tcttccactg aggaaaccaa cgcatgaccg atcccaccca gcatggcatg
      301 atagggccag acggtgaggg cgaatttgcc tttcttttcc aattcttccg cgtaatggag
      361 catcatctgc tggccgtatt ccggcgcaat gccgaattgg ggcgcaagcg cagggttgaa
      421 ggtccatttg ccgtcacgta gttcaacggc gtggatatcg gtataaggag cggggtgatt
      481 tccattttga tcgacaaaaa agatggcgtg aaaaacctgc atggtcatat gcgtatccat
      541 cgtggcagtg atctgggtaa tatttcccag attgcgatag ataaactcac aaagacgccc
      601 gttatcttcc acggcgccgc gcccgcttct gccgcccacg taaagttcaa agtctgggat
      661 gcagaaggtg ttttgtacgt cgatcagcat cagccaggtt ttggtcgaat cagcggaagc
      721 gggtccaatt ccatgctgac gtgcccagtc acgggcctgt ttcgcccgtt cttcgtaggg
      781 aatccgccag accgtgccta ctttgtttgg atcaaaaaaa tcagggatgg gaacttcagg
      841 tgtattcata gcgtctgact cgattgtaag ccctcatatt tttctgtcaa ggtatcggtg
      901 tgtggttgac tttttagctt gaaaccttta cactcggtgc aaatcatctg cgattttttg
      961 gaggagtacg tatggcgtcc catttccaac tcatcatgcg gtccggaccc acgcctggcg
     1021 ccgtcttcaa cctgggaggt gagcagctca ctgtcggccg tgattcgacg aatgatgttg
     1081 tgatcaacga tgcggaaatt tcacgccgac atgcacgcct gacctttcag ggtgggaagt
     1141 atattctgga agaccttggt tcaacaaatg ggacgtttgt gaacggtcag cgcctggctg
     1201 gtcctcgtgt attgaaagca ggcgaagttg tttcatttgg ggaacagatc gtgctggtct
     1261 atgaagcctc cacgttcgac ccggctgcta ccatcgcttc gccgcgtgct acggctgtgc
     1321 cttcggcctc gcgccccatc tcggctcctc cgcccgcccc ggctgactat gccggaagta
     1381 tcccagccag ccccgtggtt cccccctctg cccccgccag aacaaatttg actcccattt
     1441 tgatcggtgt tggattttta ttgttcctct gcacttgtgc tggaattctt tggttcatcg
     1501 acgcaaactt cctctggtgt acttttttcc ccttcctggg cggttgctga tatcaataag
     1561 atcgccccgc aagcttttca tcttgcgggg tatttttatt tacagcgtgc ccgataatta
     1621 tctaaacctg ggctgtatcg tttttgtttt ataacaggtt aataactcat aagaccaggg
     1681 gcaaatggtt gcaaaagctt cctcacttga ctacagagtg ccgcacgcat gactgacatg
     1741 cgtgtgtaaa gctgagattt tgttttccgc ttaagcatat aaaagtttac ttgcgtatgt
     1801 agcacatgcg tgagacaagc ggtagaatat agattatctt gccggagcat tttcatgaat
     1861 gacatcacct caacttcacc acgttggagc cccacaacca aattgctggt tgggctggtc
     1921 gttgttggca tcgttgcatt cctgctttat cggttttcaa acctcatcac gccgctgttg
     1981 atgatcttca tcctggcgta tcttttccac ccgctcgcct ccgggatttc gaatagtctg
     2041 caaatttcgt ggagagcggc agtcaatctt ctttatctgc tgatcgttat ccttttcatc
     2101 ggactggtta cgctgggcgg tgtgggactt gttcaacaag tgcagagcct gattggagtg
     2161 attcaggata tccttgccaa ccttccagaa tatgtagaga acctctccgg gcaagtcttc
     2221 caaattggtc cgttccgatt ggatatgcgg acgattgacc tcaacgccct cagtcagcag
     2281 gtgctttcgt ttgttcagcc cctacttggg cgaacgggaa ctctggtcgg cgcgctggcg
     2341 agcggtgcgg cagaggccct gggctggacg ttctttgtcc tcctcgtatc ctattttttg
     2401 atggcagaaa gcagcggctt gcaaagcaac ttgcttcgag tggatgtgcc tggatattcc
     2461 cgagacctgc gtacaattgg tggtaaattg agtcgcattt ggaatgcgtt cctgcgcggg
     2521 caaattttta tttttcttct gtcaacagtt atctatatca tcctgttatc tctactgggc
     2581 gtgagatatg cactggggct ggccctcatg gccgggcttg caaaattttt accttatatc
     2641 ggtccagcga tcacctgggt ggtcatgggt ctggtcacat tctttcagcc cggaaaaccg
     2701 tttgatctct cgtccctcgc ttacacagcg attgtcgtca tcattaccct agtcatcgat
     2761 cagatcattg acagtttcat cacgccccgc atcatggcac gtacgctaaa agttcaccca
     2821 gccgcggtat tggtcacggc cttggtcgcc gccaatttgc ttggtctatt gggtgtcgtc
     2881 attgctgcac ccttcctcgc atcagtgact ttattgggaa gatatacaat gcgtaagatg
     2941 tttgacctgg agccttttcc ggaaagagaa accgaattgc cttcgcctgt cgactctgaa
     3001 tggatcagac agatcagaaa actatggtct gcgacattgc agcgacggaa acgcgaactt
     3061 atagaatcaa aaagaggaga tcgtaatgag taataatcat actgaaccaa aaacggaaac
     3121 catcgccgaa accgacaact tcctggcatg gaaggcagaa gaaccagatg gcgagacaac
     3181 gtatcatatt gagctaaata acgttaccct gcacttcttt caggaagaat ggaacgagtt
     3241 cctgcagctc gttcgcgatt taggatagct gatttgctct cgccgcggtc cctcgcctgc
     3301 actgctcgca tgcgcaagtg tgcgaagcat ccgcgtggga cggcggcgag aactccgcca
     3361 tgagcattga tgaagctcaa gaatgcgatc acggatgtac gcggaattga ggtgggacat
     3421 gcacaggatg agcaagcact gacaggttgt actgtaattc tttgccgcaa aggcgcagtc
     3481 gcgggcgtgg atgtgcgcgg cggcgcaccc ggaacgcgcg aaacggatct gcttaacccg
     3541 atcaaccttg tggggcaagt ccacgcaatt gttctggcag gcggctctgc ttttgggctt
     3601 gatgcagcct ctggtgtaat gcgttttctt gaagaaaaca aaatcggatt caacaccggc
     3661 gcagcaagag tcccgattgt cccttccgcc attctgtatg acttgaacct cggtcgtgca
     3721 gacgttcggc ctgattccgc catgggatac cgcgccgcgg cagcggcctc ggctctcgct
     3781 cctcaagagg gaaacgctgg cgctggaatg ggagcttctg ttggcaagat gtttggctcg
     3841 aaacttgcaa tgaaagccgg gttgggaaca gccagcctgg caatcggcgg tggaatcgtg
     3901 gtcggcgcaa ttgtggcggt caatgccttt ggagatgtca ttgatccgca cacgggaagc
     3961 atcatcgctg gcttgcgttc ggggaaagta ggtccggtac ggattggcaa aaaggatcat
     4021 tttgcagata cacttgcggc aatgaagaat ccggttggac gcggcgttct aagtttggca
     4081 tcccatacga acacggttat tggtgtggtt gctacaaatg caaaattgac gaaggcacag
     4141 gcaaccaaag ttgcacaaat ggcacaggat ggacttgcaa gagcgatccg acctgctcat
     4201 accatgctgg atggtgatac catcttcgcg ctatcaacgg gaaacaggaa ggccgatatt
     4261 tcaacgattg gtgcgttcgc agcagaggtc atggcacaag cgatcgtgcg tggagtaaag
     4321 atggccgtgt ccgccggcgg g
//
LOCUS       DASTJP010000704         5375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_108913, whole genome shotgun sequence.
ACCESSION   DASTJP010000704 DASTJP010000000
VERSION     DASTJP010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108913"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFQ23_20560"
     CDS             <1..760
                     /locus_tag="VFQ23_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909052.1"
                     /translation="YAPPSEGPIPSSRALYTMPTDGSREPELLFQPPTPDDHYIHVEW
                     SSDGQYIYYVHYNNTERAAGELYPAYELLRMTYPDGQPESIAQKAFWPRLSADSSRLV
                     YVSLDPATGLNELFLANADGSNAQKVNINGSYISDVIDAPIFSPDGTSILFSAPPPPQ
                     AYQPNWFDKLTGVQIVKAHAIPSDWWSVPVTGGELTRLTQIQTINLFASASPDQVWLA
                     SVSGEGLFAMNWDGSNLTQLLFDPGITGTVNWIP"
     gene            1013..2782
                     /locus_tag="VFQ23_20565"
     CDS             1013..2782
                     /locus_tag="VFQ23_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909053.1"
                     /translation="MQKRFSRTIASLLAVFVIFTTAAAPGGNNKSGFKTAQGSMLDPV
                     MAGVEVTPILTVGDVLPSGFRFEAVPDGIALRTRGQGRVDVFVNHETSKVPFPFNPAN
                     PTAANGENDFDNAQVSQLIFNQHSGGVLNGSFIIDSSSGYQRFCSSYLATSKEGFDRD
                     ILFTNEEAIDYVFRQEDSWPPPIGDPNERQVGWVVALDVQTGQHHPIYGMGRHNHENS
                     VPIPGFDELAVISGDDTFTSGPLTIPAGGPLPAGSRPAQSKLFSYIAADTDSLLADEG
                     ELWGFVSDNPSFNDYYDFTPGSTQTVSGHFVKVPKNIATGLDSDGSELKAADVGFPLP
                     PNDGSWQRDNRTTVPTGLDGPQWVLEYWSQLNNVFQFVRVEDIAYDKRPGMENVVYVV
                     DSGRGTAGVSQAGRSTNGRVWKMVFDPNDPTVVTSLSIFVEGDDNPVKTLAEVHQPDN
                     IESTPTGILLTEDPGSSQQFPVGSADPAATTARLMWVPFVGNGAGAAQTVVKVNQSAD
                     EGPTDVDPNTAPGNWGAWESSGIVDASEAFGPGAFLIDVQAASLWVESAPGFDTFIDA
                     NSDPDFTFKRAGGQLLLIRIPGL"
     gene            2891..4966
                     /locus_tag="VFQ23_20570"
     CDS             2891..4966
                     /locus_tag="VFQ23_20570"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9909054.1"
                     /translation="MLLFAISIFLSAFLLFQIQPMIGKFILPWFGGTPAVWSTVMLFF
                     QVLLTGGYAYAYWLIARVKPKKQSTIHISMITLAIFILICLSFSWTSPITPDASWKPA
                     DIDMPVRDIFKLLVISVGLPYFILASNGPLMQAWFSNIFPDQSYARLYALSNLGSLLG
                     LLVYPVLIEPNLPLRGQGWGWAIGFALFGILASWIALRSSGQSPLAKPIADPVKSTQR
                     PAFALMSLWILLSTTASLFLLSVTNQISQEVAVIPFLWIVPLTLYLLSFIMTFSGERG
                     YNRKFYAVLFMIFTAFTLFVMLNATALHVYWQIVAYCLLLFAVCMLCHGELYLLRPPA
                     NHLTTFYLMVSIGGALGGLFVSLIAPVIFNGYWEFFVGLAMAVSVLLTVLRAGQSRNI
                     TARARFVFTTFGLVTMMLAVLSLVSSEISFSKRNFYGVIRVHTATPSGSTLQANIMSH
                     GITVHGLQFLTGFKTSNIPTTYYVRDAGAGLAILNHPKYGENMQVGLLGLGVGTLLAY
                     SQPGDTYRLYEINPAVIDLAAGVGDYFSFVSDSPAEITVVPGDARISLERELAKGNAQ
                     NFDILVLDTFSSDSIPVHLVTREAFALYLEHLAPDGIIAAHITNLHLDLQPVFWQLAM
                     YYGLSMVRINYEGDSNGGYASHWILLSRDPAVLHIPAIQARSVDLTDYSTAVRLWTDD
                     YSNLFQILK"
     gene            complement(5002..>5375)
                     /locus_tag="VFQ23_20575"
     CDS             complement(5002..>5375)
                     /locus_tag="VFQ23_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909055.1"
                     /translation="GLIVPAAEIHAVAKDDFMAGFRVREWWPIFRQNWGGFVVAMAIL
                     YALLTAMSFAMQFLIITVVLFCLLPLLIPVISMYSAIIQFSTFAQAYREGRDKLSVVS
                     AQALTTGETDLQIQTSEQSKG"
BASE COUNT         1225 a         1378 c         1323 g         1449 t
ORIGIN      
        1 ctatgcaccg ccatcagaag gtccgattcc ttcgagtcgg gccttgtata caatgcctac
       61 cgacggatcg agagaaccgg aattgctatt tcaacctccc accccggatg atcactatat
      121 tcatgtggag tggtcctcag atggacagta tatttattat gttcattaca acaatacgga
      181 gagggcggca ggcgagcttt acccggcgta tgaactgctc cgaatgacat atccggatgg
      241 tcagcctgaa tcgatcgcgc aaaaagcatt ctggccgcga ctttctgccg actcgtcccg
      301 gctggtttat gtatcccttg atcccgccac gggtctgaat gaattgtttc tcgccaatgc
      361 agacggaagt aacgctcaaa aggtaaatat caatggatcg tatatttccg acgtgattga
      421 tgccccaatt ttctccccgg atggaacctc cattcttttt agtgcgccgc ctccacctca
      481 ggcgtatcag cccaattggt tcgataagtt aacaggggtt caaattgtga aagcacacgc
      541 gatcccttcc gactggtggt cggtgcccgt taccggcgga gagctcactc gcttaactca
      601 gatccaaacc atcaatttat ttgcgagtgc ttcgccagat caggtttggc ttgcgagcgt
      661 gagcggcgaa ggcctgttcg caatgaattg ggatgggtcg aatcttacac aattgctgtt
      721 tgatcccgga atcactggaa ccgtcaattg gataccgtaa tgcagtttct tgttgtgtaa
      781 cattttctaa ttagaaatct ctcacctact gttgaccttg catccaaggg tgagtagaat
      841 aaattcgaaa attcatgaca atatacacat aagtcagatt ctcaaaaaac tcgtttacga
      901 caggaggtga cgggcaaaat tcgagcagtt aacgaggaga tatggttccg cacttgccat
      961 gcgacagatc ataaatttct aaccacccat agtttgtttg aggagacgca gtatgcaaaa
     1021 gagattttct cgcacgattg caagtctatt ggctgttttc gtgattttca caaccgcggc
     1081 ggcaccggga ggaaataaca aaagtggttt caagacggca cagggttcga tgctcgaccc
     1141 agtgatggcc ggtgttgaag ttacaccgat tctgaccgtt ggggatgtgc ttccgagcgg
     1201 tttccggttt gaagccgttc cggatggtat cgctcttcgc acccgagggc aggggcgggt
     1261 ggatgtgttt gtgaaccacg agaccagcaa ggtgcctttt ccgtttaacc cggcaaaccc
     1321 cacggcagcc aacggtgaaa atgattttga taatgcccag gtgagccagt tgattttcaa
     1381 tcagcactca ggcggtgttc tcaatggctc atttatcatt gatagcagtt caggttatca
     1441 gcgtttttgc tcaagctatc tcgccaccag caaagagggc ttcgaccgcg acattctctt
     1501 cacgaacgaa gaggcgatcg actacgtttt ccggcaggag gattcgtggc ctccaccgat
     1561 tggcgaccca aatgaaagac aggttggttg ggtggtcgct ctggatgtcc agaccggtca
     1621 acatcatcca atctatggaa tgggtaggca caatcatgag aatagcgttc ccattccggg
     1681 cttcgatgag ctggctgtaa tttctggcga tgatacgttc accagtggtc cacttacaat
     1741 cccggctggt ggtccattac cggcagggag cagacccgcg caatctaagc tcttctccta
     1801 cattgcagcc gatacagata gccttctggc tgacgaaggt gagttgtggg gatttgtttc
     1861 agataacccc agctttaatg actattacga cttcacgccc ggttcaaccc agactgtgag
     1921 cgggcacttc gtcaaagtgc caaagaatat tgccaccgga ctggacagcg atggctccga
     1981 gctgaaagcc gcagacgtcg gcttcccatt gcctccaaac gatggttcct ggcagcgcga
     2041 caaccgcacc accgttccta cgggcttgga tggtccacaa tgggtgctcg aatactggag
     2101 tcagttgaat aatgtgttcc agtttgtccg cgtcgaagac attgcgtacg acaagcgccc
     2161 tggtatggaa aatgtggtct acgttgtgga ctctggccgc ggtacagcgg gcgtttctca
     2221 ggccggtcgc tcgacaaacg gccgcgtctg gaagatggtc tttgacccga acgatccgac
     2281 ggttgtcacg tccctttcca tattcgtgga aggagacgat aaccctgtaa aaaccctggc
     2341 tgaagttcat cagcccgaca atatcgagtc gaccccgacc ggaattctgc tgaccgaaga
     2401 tccgggcagt tcacagcaat tccctgtggg ctcggcagat cctgctgcga caaccgcgag
     2461 gctcatgtgg gtgccatttg ttggaaatgg agccggtgct gctcaaactg tggtgaaggt
     2521 caatcagtct gctgacgaag gtcctaccga cgttgatccc aatacagcac caggcaactg
     2581 gggtgcctgg gaaagctctg gcatcgttga tgcctctgaa gcgtttggtc cgggcgcctt
     2641 cctgatcgat gttcaggccg cgtccctttg ggtggagagc gccccaggct tcgatacctt
     2701 tatcgacgcc aatagtgatc ctgacttcac gttcaaacga gcaggtgggc agctgctgct
     2761 aataagaatt ccgggtctat aaaccgatac agcccaattt cactacttaa caaaactggg
     2821 tgtccgttat tatcgacggt acacccagtt ttattttctt cgatgtagaa ttgaccagtt
     2881 acaaattcgt atgcttctct tcgccatcag cattttcctc tcagcatttc tcctctttca
     2941 aatccagccg atgatcggca agtttatttt gccctggttt ggcgggacgc ccgcggtgtg
     3001 gtccacggtg atgctgttct ttcaagtgct gctcacgggc ggctatgcct atgcttattg
     3061 gctgatcgcg cgtgtgaaac caaaaaaaca gagcactatt catatttcta tgatcacgct
     3121 ggccatcttc attctcatct gtctttcttt ctcctggacc tctcccatca ctcctgatgc
     3181 aagctggaag cccgctgata ttgatatgcc tgtgcgggat attttcaagt tgctcgtgat
     3241 ttccgtcggc ttgccctatt tcatccttgc ctcgaatggt cctctgatgc aggcctggtt
     3301 cagtaatatc tttcctgatc aatcctacgc acggttgtat gctttatcta acctcgggtc
     3361 tttgcttggc ttgctggtct atccggtttt gatagaaccg aatttacctc ttcgcggcca
     3421 gggatggggc tgggcaatcg gcttcgcact attcggcata ctcgccagtt ggatcgcatt
     3481 gcgcagcagc ggacagtcac ctctcgcaaa gccgattgct gatcccgtta aatcaacaca
     3541 acgcccagcc ttcgctttga tgtcgctatg gattcttctc agcacaacgg cttcattgtt
     3601 tctgctctct gtgacaaatc agatctccca ggaagtggcg gtgattccat ttctttggat
     3661 cgtgccactt acactctacc tgctttcctt cattatgaca ttttcagggg agcgcggcta
     3721 taacagaaaa ttctatgcgg tgttattcat gatattcacc gcgtttacat tgttcgtcat
     3781 gttgaacgcc accgctctgc atgtgtactg gcaaatcgtc gcctactgcc tcctgctatt
     3841 cgctgtctgc atgttgtgtc acggagaatt gtatcttttg cgtccccctg cgaatcatct
     3901 cactacgttc tacctgatgg tctcgatcgg aggtgcgctt ggcgggttat ttgtcagctt
     3961 gattgcgccg gtcattttca atggttactg ggagttcttt gtcggcctgg cgatggcagt
     4021 ctcggtcttg ctcacggtcc ttcgcgcagg tcaatctaga aatatcacag ctcgggcacg
     4081 atttgtgttc actacgtttg ggttggtcac catgatgctg gcagtgctca gtttggtctc
     4141 ctccgaaatt tcattttcca aaaggaattt ctatggcgtg attcgggtgc atacggcgac
     4201 ccccagcggc tcgacccttc aggccaacat aatgtcacat ggcatcaccg tacatggact
     4261 tcaatttctc accgggttca aaacgagcaa tataccaaca acctattatg tacgcgatgc
     4321 cggtgccggg ctggcaattc tcaatcaccc gaagtatggt gaaaatatgc aggtcgggtt
     4381 actgggactc ggggtaggaa ctctcctggc ttactcgcag cccggcgata cgtatcgtct
     4441 gtacgagatc aatccggctg tgatcgattt agcagcaggg gttggagact atttttcttt
     4501 cgtgagtgat agcccggcgg agatcaccgt cgttcccgga gatgcgcgaa tctccctcga
     4561 acgcgaactt gcgaaaggca atgcgcaaaa ctttgacatc ctggtattgg atacctttag
     4621 cagcgactcc attccagtcc atcttgtaac gagagaagcc tttgctttat atcttgaaca
     4681 tcttgcaccc gatggcatca ttgccgctca tattacgaac ctgcatttag acttgcaacc
     4741 ggtcttctgg caattggcaa tgtactatgg tttgagcatg gttcgcatta attatgaggg
     4801 cgactcaaac ggtgggtatg catcccattg gattctgctt tcacgtgacc cggcagttct
     4861 acatattccc gctattcagg ctcgttcggt ggatctaacc gattactcga ccgctgtcag
     4921 attgtggacc gatgattaca gcaatttgtt tcagattttg aagtagcgta tattactgcg
     4981 aagtgagaag agaccgacgt atcaaccttt agattgctct gacgtttgga tttgcaagtc
     5041 ggtctcgcct gtggtgagcg cttgcgcact tactacactc aacttatccc taccttccct
     5101 atacgcctgt gcaaatgtcg aaaattgaat aattgcagaa tacatggata tcactggtat
     5161 tagtaagggt aataagcaaa acaaaactac agtaataatc aggaattgca tcgcaaacga
     5221 cattgccgtc agtagagcat ataggattgc catggcaaca acgaatcctc cccagttttg
     5281 cctgaagatc ggccaccatt cccgaacgcg aaacccagcc ataaagtcat ctttcgcgac
     5341 tgcatgaatc tctgctgcag gaacgatcaa tccga
//
LOCUS       DASTJP010000705        16114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_371081, whole genome shotgun sequence.
ACCESSION   DASTJP010000705 DASTJP010000000
VERSION     DASTJP010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16114
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371081"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..8811)
                     /locus_tag="VFQ23_20580"
     CDS             complement(<1..8811)
                     /locus_tag="VFQ23_20580"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9909056.1"
                     /translation="MAGKSSSLSKVIKSKRRVGIKSETSKASGLKDSSQKKSSELKQQ
                     LAQREDELQIINSIQQGLASKLDVQAIYDLVGDKIRDIFDAQIVTLVSYDRAAGKNLI
                     HYAIENGKRHTYGSQPTTDLYRELIATGKPIIIQEKLAERVRHKAQPMDDSAGHLSGV
                     PKSGVWIPLSVSGTVTGHVSLQNVDRENAFSDSDIRLLTTLANSMSVALESARLFKAE
                     QERSAELAIINSVQEGLAAELDMQSIYELVGEKIREVFNADSNYIGLYDHANQLVHPQ
                     YAIDRGFRLKFDRPFQMGHGFYTHVIRAQKPLIVNTIEDGAQLGGIPTPRPDTGQDLN
                     ESYLGVPLMLGSEVKGVVAVQSYKKFAFDESDARLLSTLTNSMSVALENARLFDETQR
                     LFKAEQERVAELQIINSIQQGLAAELDFHAIIDLVGEEIRNLFAVDTIFISLYDQKTQ
                     WIHFPYYLENGIRTHPDPYQLGTGLSSHVVNTRQPLLINENAEQRFKELGAVYLPNEN
                     PKKSWLGVPIVSGNIVIGTITLQDVNENVFRNSDVRLLTTVSASMGVALENARLFDET
                     QRLFKAEQERVTELQIINSIQQGLAAELDFQSIVDLVGDKLREVFNTSDMAIRWYDEE
                     ANLIHFLYEYEHGERLKLEPIQPRAEGPFEEMRRTRLPLILNTPEDFLKKGLAAIQGT
                     DSGKSMITAPIIGSDRVLGVISIENYERENAFGDSELRLLTTIAASLGTALENARLFD
                     ETQRLLKITEERNAELAVINSVQLALAAELDMQGIYDAVGDKIRDIFDAQGVIIGTLD
                     YENKQTKFDYFYEKGQRYFPETIPFSGLMEHIAQTGEMVVINENMLERVKEYRMTVAA
                     GELARSGVWMPFKAGTVVRGIIALQNIDRENAFSASDIRLLQTLANSMGVAIENARLF
                     DETQRLLKETEQRNTELAIINSVQAGLVAKVNIQAIYDLVSAKIRETFNVNTVAIETY
                     DHVAKTSTLHLTWESGEPVRKNPEPFNKIDERLITTRQPFLVNKNVAETMRQLGVELG
                     TLDDPKMSIVWIPLIIGDIVIGNIGLQDDDKENAFTESDVRLLQTLANSMSVALENAR
                     LFDETQRLLKETEQRNAELAIINSVQAALAAELNIQGIYDAVGDKIRSIFHNTDLNIR
                     ILDPNTNLVHFPYFYEGGRQIEVDPIELTDKGVTPHILRTRKILVINENLEEETNKYG
                     SFTLPGTEIEKSAVFVPLVIGDQARGLINLSNMEHENAFSDSDVRLLETLANSMSVAL
                     ENARLFDETQRLLKETEERNAELAILNSVGEAMAQTLDVKTLTSIVGEKVQNIFSSDI
                     VYIRLLDPKANLLHQYYNYDMGPMQLDQPIPFGEGMSSKAIQTKQPIVFETDQESREL
                     GAIPSTMPTAKGSMKDSESGVIVPILTADKAIGVISVQSYRKHAFTKNNLKLLQTITA
                     SMSVALENARLFDETIRHARESAALNQVGRDISSTLNLSSVMERIASHARDQLQADTS
                     AIFLPESNGSVYKTIVAQGVNAAEIKAHIVQAGEGIIGTLAQQGEAEFINDTNKDPRV
                     VQIRGTPKQEQERLMVAPLLTGDKVSGMMAVWRQGGEIFAQADLEFLQGLSLQAAIAI
                     KNANLFDEIEQRAAELAIINSVQKGLASRLEMQAIYELVGEKIREVFDAQVVVINSFD
                     TEKQLTIMRYGVEKGNRFYNDPYTLTEGHHRLIKKRQPLLINEDWAERMSEYGFTINI
                     VPGTEMPKSTVIVPLIVSSEVKGSVSLQNVDRENAFSEADARLLTTLTNSMSVALENA
                     RLFDETQRLLKETEQRAVELAIINSVQQALAAKLDMKGVYTTVGEKLREIFSAQAIII
                     YSGNLKTRLETQEYYFEKGQKLERITEPLNSMYEYVLGLNKTFVRNKDFPQFAAQFKD
                     YVIPQGEVPISVINTPVIRNEGDGYYLNISIMDLNGEKTFDESDVRLLETLASAMSVA
                     LENARLFDETQRLLKETEQRAAELAIINSVQAGLAQNLDIGAIYNVVGDKIREIFDAQ
                     TVMLTSYDKSATSIISRYLIEKGERFYPPPVAINKLHQNLISNRKTFIFNENTDEELT
                     NLGAVVVPGTEIVRSAVYVPLLAGKHVFGMISLQNVDHEHAFSESEVRLLETLAASMS
                     VALENARLFDETQRLLRETEQHASELSTINTVSNALAGELDLSALIDLVGEQIRNAFT
                     ADIAYVALLDEKTNIINFPYEHGQHLEPLPLGEGLTSKIIEKGEPLLINQDVDRRREQ
                     LGATQIGVQARSYLGMPIFVRGKAIGVVSVQSTTQEDLFTEDDQRLLGTIAANVGVAL
                     QNARLFDEIQTRNREVTESLEQQTATSEILRVIASSPTDIRPVLDAVATNAARLCEAN
                     DVQIYKVDGNLLRQVTHFGPLPALEDGEGLPLVRGLVTGRAVLEHRTIHIEDMLELSE
                     AEYPNSVALQKRLRHRTTLAAPLLREGMAIGAIVIRRNEVHPFSEKQISLLGTFADQA
                     AIAIENIRLFNETQHLLKETERRAAELAILNNIGEAMTKALDIKSLTRNVGDKVREIF
                     NAEIADILLYDAQSNTVHLAYSYSNGYFEDEPPWNLEDGGLTSKIILTSQPLLLSTAK
                     ELEENGAAAYVTTLDESEEIQSYLGVPILAGDKVLGVVDVQSARHSAFNEDNLRLLQT
                     LATSMSVALENARLFDETQRLFNEAQLARAAAEQANEAKSSFLATMSHEIRTPMNAVI
                     GMSGLLMDTELNKEQRDYAETIRNSGDALLAIINDILDFSKIEAGKMDVERQPFDLRE
                     CVESALDLTAAHAIEKGLDIAYLMEDDVPVGIKSDATRLRQILINLLSNAIKFTEKGE
                     VVLTVRKGKAKNELLFAVRDTGIGISESHMSRLFQSFSQADSSTTRRFGGTGLGLAIS
                     KRLAEMLGGEMHAESEGVGKGSNFFFTIKAESAKVTERKTARDIKGIQSIL"
     gene            complement(8837..9040)
                     /locus_tag="VFQ23_20585"
     CDS             complement(8837..9040)
                     /locus_tag="VFQ23_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909057.1"
                     /translation="MKPTKATNVVDLRQIIFQLLWACQFISSTGSTLVLLKRREKSNG
                     HVAPDQPDMKEEPASARGWSMIS"
     gene            complement(9094..10095)
                     /locus_tag="VFQ23_20590"
     CDS             complement(9094..10095)
                     /locus_tag="VFQ23_20590"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET9909058.1"
                     /translation="MSQREDILGLLSGNTRTNQPVFSGLIHITTEGLEGDGLVLHEVH
                     KDAKRMARAAASTFRLTGMPSAALPLDLCAPAEALGSTLHYYEQEKDQFPQPAKPLFA
                     STKYLNSAYFENADFVNKGRIPLICDAIRLLKEEVGGEVVISGIIPGPYTLLLYLIEP
                     GGLFAEMKREPNAVLDALVHLSAFLAEIGKAYTDAGADYITIHDMGGSPGFIGPTKYE
                     QFAFPPEKQLIENLQKPCVLSVCGNTNKSMYLLAQTGANAISVDQVNDLSASREALRD
                     TLLFGNIDPVKVLWQGDEVSITDAIRVAKEAGVDAIWPGCDLVPSTPIQNLAIMLKG"
     gene            complement(10116..11018)
                     /locus_tag="VFQ23_20595"
     CDS             complement(10116..11018)
                     /locus_tag="VFQ23_20595"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET9909059.1"
                     /translation="MNKFLERLNSDEILVADGATGSNLQKMGLKPGKPPEDLIIDAPD
                     TILKLASSFVEAGSDIVLTCTFGGTRMRMKDSKYQDRTPEVNIRAVEIARKAASAREG
                     VLVAGSMGPVGLLIKPYGPLEAEEVKATFAEQAKALADGGVDLLLIETMFAFEETTAA
                     FEGARSATDLPIVISFSYDRGTRSMMGVKPKDVIKKYSEMGATLVGANCGTTLENMEA
                     VVKEYSETVANFPLWVKPNAGVPRMDIETEQGVYDMGPDDMAKFSQRYVELGARVVGG
                     CCGNTPEHIAAIVKAVKRQPVTSE"
     gene            complement(11149..13182)
                     /locus_tag="VFQ23_20600"
     CDS             complement(11149..13182)
                     /locus_tag="VFQ23_20600"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HET9909060.1"
                     /translation="MTKKHTIILQPSGRRGQVDEGTSIRAAARELGVEIESICAENAT
                     CGKCMVLIEEGRFEKYNIDSRREHLSPVGTEERAYLMRRPKLLKDKGWEIGQVRLSCQ
                     CKVLGDVLINVPEESRGNKQIVRKSARERAIEIKPSVRKYYVSMSPPNLERPIADWER
                     LAKGLETSMGLVRRGEENLPRWFDLDIDYQCLRTLSSTLRAAKWNVTVSVWQDKEVIE
                     VQAGYVEDSYGAAVDIGSTTVALYLCNLRTGELLAAESEMNPQIVYGEDVMSRIQYTI
                     EHADGLEKLHKAVIQTLNKLLKQAAQTAFIKTDEILEMVLVGNSTMHHLLLNLHPKDL
                     GLAPFVPAIHKSVDVKARELGLQINPSGNIHVLPTIASFVGADTSAMIVAEEPHKQDE
                     NWLLIDVGTNAELILGNRKRLVCTSTPTGPALEGAHVEYGMRAAPGAIERVHIDEATL
                     EPKYKVIGVEGWNTDHAEFKGHVKGICGSAIIDSVAELFRAGIVDSRGKFKKNLDSKR
                     IREGENGWEYVVAWADETSIGRDIPMTQQDVRQIQLAKAALFTAARTLLKRSNLQSPD
                     KIILAGGFGSYIDKEKAMLIGLIPDCKLENVYAVGNAAGDGARIALLNVEKRKEIDSV
                     TRKVERFELPTDPEFQNQFMLATSFPHMSEPFEHIAHLIPNRVLDPLAKNFMK"
     gene            complement(13249..13539)
                     /locus_tag="VFQ23_20605"
     CDS             complement(13249..13539)
                     /locus_tag="VFQ23_20605"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HET9909061.1"
                     /translation="MARYRIMYWKHIPQSFTVEGDGRTVKKQLSEKIQNKIDAYAMAE
                     GLTSTSDYSAQYKRGEWIECDGAPEEVAEALLLELETEFAKLPIPKRNGNHA"
     gene            13816..>16114
                     /locus_tag="VFQ23_20610"
     CDS             13816..>16114
                     /locus_tag="VFQ23_20610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909062.1"
                     /translation="MSDMGKFRGTSLRLWFMIALVIGSAIPVHIRASAQQSPAPLWVV
                     QSLSTSEYGVIAPQGLAFSADEKAFLVLDGTSDVTLITIADEPAGKKAIADVGSDPLN
                     TAFDKRTNSLVVLDRGRGELAKVKEDGSSMRFAIGGLKLADPQGITINPANGQMFVLD
                     AGKAYIVSVPTHDTLGVDASKMQQISLQGLGAGKFKGLAYNPNNGHLYISETTQKKLY
                     EITQSGELVNTFDLTAVEISNPSAMTFAPSVDNTDDPNIYDLFILDTTSSGQIVELSL
                     QQPAALPSGTTLLPANMVNIIDTSNQAWNPSAPDPAGLDYWPAANRLVISDSEVDEMP
                     PYFQGKNVFLSTTSGSLTGTCSTTAYTGEPTGVGVNPNNNHIFIAADFQDRLFEISLG
                     PDGQYCTSDDTFTNTSLATAYGVTDAEDVAYGNNTVFIAGGADAEVFVIPLGANGVVG
                     GGDDGPMTHWDTAALGFADAEGIGFNHDAGTLFVVSTKRTDKYLGEFSTTGTLLRAYN
                     LSFMPDVGNIRSDVTYAPGSQNPLVKNIYIASRGIDNDSNPNENDGKIWEINIGSAPS
                     ATNTPGPSPTFTNTATQTSTPTITPTSTAIVPPSGNSFHTSFGSGGTLSGVAFADDDI
                     MHFDGSTWSLYFDGSDVGLSSVDILAIQSLDADSLLLSFNTAITLNGIAFASTDVARF
                     DATSLGSNTAGTFSMYFNGADVDLSASGENIDAFEILPDGKLLVSTTGNPTVTGVSGA
                     RDEDILQFTPTTLGTNTSGTWSLYFD"
BASE COUNT         3762 a         4060 c         3859 g         4433 t
ORIGIN      
        1 cagaatagat tgaataccct taatatcgcg tgctgttttg cgctctgtga ctttggcgga
       61 ttccgctttg attgtaaaga agaaatttga gcctttgccg acaccctcac tttcggcatg
      121 catttctccg ccgagcattt cagcaagacg tttgctgatt gcaagaccca gacccgtgcc
      181 gccaaaccgc cgcgttgtgg atgaatcggc ctgtgagaat gattgaaaga gccgactcat
      241 atgactctcg gaaataccta tgcctgtatc gcggaccgcg aatagcaatt cattcttcgc
      301 ttttcccttt ctaacagtga gaaccacttc gcctttttct gtaaacttga tcgcattgct
      361 gagcaggttg atcagaatct gccgcagacg ggtcgcatcg cttttaatgc caaccggtac
      421 atcatcttcc atgagatagg cgatgtccag tcctttttcg atcgcgtgag ctgctgtcag
      481 gtccagtgca gattcgacac attcccgcag gtcaaagggt tggcgctcca catccatctt
      541 tccggcctca attttgctga agtccagaat gtcattgatg atagcaagga gcgcatcgcc
      601 tgaattgcga atggtctctg cgtaatctct ttgttcttta tttagttcgg tatccatgag
      661 caaaccgctc atgccgatga ccgcattcat cggtgtgcgg atctcatgac tcatcgtagc
      721 caggaaggaa cttttggctt cattcgcctg ttcggcggcg gcgcgggcca actgagcctc
      781 attgaatagg cgctgggttt cgtcaaagag gcgtgcgttt tcaagggcaa cgctcatcga
      841 ggtggcaagg gtttgcaaaa ggcgcaggtt gtcttcatta aatgcgctgt gtctggcact
      901 ttgcacatcc accacaccca ataccttgtc gcccgccagg atcggtacgc ctaaataaga
      961 ttggatctcc tcactttcgt ccagcgttgt cacataggca gccgcgccat tttcttccag
     1021 ttctttcgca gtgcttagca gtaatggctg gctggtgagg atgatcttcg acgttaagcc
     1081 accgtcctcc aaattccagg gaggctcgtc ctcaaagtag ccgttgctat agctataggc
     1141 aaggtgcacc gtgtttgatt gtgcatcata caggaggata tctgcgatct ctgcattaaa
     1201 gatctcacga actttatcgc ccacattgcg agtcagagat ttgatatcca gagccttcgt
     1261 catggcttca ccgatgttgt ttaggatggc aagttcggca gcgcgtcgct ctgtctcttt
     1321 gagcaggtgt tgtgtctcgt taaagagacg tatattttca atggcgatgg cggcctgatc
     1381 tgcgaatgtt cctaataatg aaatctgttt ttcagaaaat ggatgcactt catttcggcg
     1441 aatgacgatt gcaccaatgg ccatgccctc gcgcaataat ggcgccgcca gggtagtccg
     1501 gtgtcgaaga cgtttttgga gggcaacact attcggatat tcggcttcac ttaactccag
     1561 catatcttca atgtgaattg tcctgtgttc taacacggca cgacctgtga ctaaacctct
     1621 cactagcggt aacccttctc cgtcctccag ggcggggagc ggtccgaagt gcgttacctg
     1681 acgaagcagg ttgccatcca ctttatagat ttggacgtca ttcgcttcac acaaccgcgc
     1741 agcattcgtt gcaaccgcat ccaacactgg tcgaatgtct gtcggtgaac ttgcgatcac
     1801 acgcaggatt tcgctggtgg cagtttgctg ttcaagtgac tcagtaacct cacgattccg
     1861 agtctggatt tcatcgaaga gtcgggcatt ctgcagggcc acgcccacat tcgccgcaat
     1921 ggtgcccaac agccgttgat catcttctgt gaataaatct tcctgcgtcg tgctttggac
     1981 gctgaccaca ccgattgcct tgcctcgcac aaaaataggc atacctagat acgaacgcgc
     2041 ctggacgccg atttgtgtcg cacccagctg ttcccgccgc ctgtctacat cctgattgat
     2101 cagtaatggc tcgcctttct caatgatctt gctggtcaac ccctcgccca atggcaacgg
     2161 ttccaaatgc tgaccatgct cataggggaa gttgatgatg tttgtttttt cgtccagtag
     2221 tgctacatat gcaatgtctg cagtaaatgc gttgcggatt tgttctccga ccagatcaat
     2281 gagcgcgctc agatccaatt cgcctgccag ggcattgctc actgtattaa tagtggaaag
     2341 ttctgatgca tgttgttccg tctctcttaa cagacgttgc gtttcatcga agagacgggc
     2401 gttttcgagg gcaacgctca tgctggctgc cagtgtctcg agcaggcgca cctccgactc
     2461 actaaaggca tgttcgtgat ccacattctg aaggctgatc atgccaaaga cgtgtttgcc
     2521 agccagcaaa gggacataga ctgctgagcg gacgatctcg gtgcccggga ctacaaccgc
     2581 gcccaggttg gttaattcct catcggtatt ttcattaaaa ataaaggttt tccggttgct
     2641 aatgagattt tggtgcaatt tattaatagc cacaggtggt ggataaaacc gttcgccttt
     2701 ttctatgaga tatcgtgaga tgatggatgt tgcggatttg tcgtaactgg tcagcatgac
     2761 ggtttgggca tcgaagattt ctcgaatctt atcgcctaca acattataga tagcgccgat
     2821 atccagattt tgtgccaggc ctgcctgcac gctattgata atagctagtt cagcagcacg
     2881 ctgctctgtc tctttcagca gacgctgggt ctcatcgaaa agacgcgcat tttcgagtgc
     2941 gacactcata gcgctggcaa gggtttcgag caagcgcaca tcggattcgt caaatgtttt
     3001 ttcgccgttc aaatccatga tcgaaatatt tagataataa ccatctccct cgtttctgat
     3061 cactggggta tttattacag atatgggcac ttcgccttgc ggaataacat agtctttgaa
     3121 ttgcgctgca aattgcggaa agtctttgtt ccgcacaaag gttttgttca atccaagaac
     3181 gtattcatac atggagttga gcggctcagt gattcgttca agcttttgac ctttttcaaa
     3241 ataatactcc tgcgtttcca gccgtgtttt taaatttcca gaatatatga tgatggcttg
     3301 cgcactgaaa atctcgcgca gtttttcacc gaccgtggta tagacacctt tcatatccag
     3361 ctttgcggct aaggcttgtt gcacgctgtt gatgatggcg agttccacag cgcgctgttc
     3421 ggtttccttg agcaggcgct gagtttcatc gaagagtcgg gcattttcaa gtgcgacact
     3481 cattgagtta gtcagagtcg tcaagagacg ggcatctgcc tcgctgaacg cattctcccg
     3541 atctacattt tgtaagctaa cggaaccttt cacttcactg ctaacgatca gaggcacaat
     3601 gaccgtggat tttggcatct cagtgcctgg aacgatatta atcgtaaaac cgtattcgct
     3661 catccgttcc gcccagtcct cattaatgag caaaggctga cgctttttta tcaggcgatg
     3721 atggccctcg gttaaggtat aagggtcgtt gtaaaatcta tttccttttt caacgccata
     3781 gcgcattatg gtcagctgtt tttccgtgtc aaacgagttt atcactacca cctgggcgtc
     3841 aaacacctcg cggatttttt ctccgacgag ctcatagatg gcttgcatct cgagcctgga
     3901 tgctaaccct ttctgtacac tgttgatgat cgcaagctcg gccgcgcgct gttcaatttc
     3961 gtcgaacaaa tttgcattct ttatggcaat cgccgcctgc aatgacaacc cttgtagaaa
     4021 ttcgaggtcc gcttgcgcaa aaatttctcc gccctgacgc cagactgcca tcatgccact
     4081 tactttatct cctgttagca atggcgccac catcaaacgt tcctgttcct gtttaggtgt
     4141 gcctcgaatc tgaacaaccc gtggatcctt atttgtatca ttgatgaatt cggcttcgcc
     4201 ttgctgcgcg agcgtgccga taattccttc accggcttga acaatatggg ctttgatttc
     4261 tgctgcgttg acgccctggg cgacgatcgt cttataaact gatccattcg actcgggaag
     4321 aaagatagca ctggtatctg cctgaagctg atcacgggca tgcgatgcaa ttctctccat
     4381 gacagaagaa agattcagcg tggaagagat gtcgcgtccc acctggttca gagcagcaga
     4441 ttcacgggcg tgacgaatcg tctcgtcaaa cagacgcgca ttttcaaggg caacactcat
     4501 gctggccgtg atggtttgca atagtttcaa gttattcttt gtaaaagcat gtttgcgata
     4561 actctggacg ctaatcacgc caatggcttt atccgcggtt aagattggga caatcacacc
     4621 cgattcgctg tctttcatgc tgcctttggc agtcggcatg gtagaaggaa tcgcgcctaa
     4681 ttctcttgat tcctgatcgg tttcgaagac gatcggctgt ttcgtttgta ttgctttaga
     4741 cgacatgcct tcgccaaacg ggatcggctg atccaattgc atcgggccca tgtcgtagtt
     4801 atagtactga tgaagcaagt ttgcttttgg atccagcagg cgaatgtaga cgatatctga
     4861 actaaatata ttttgcactt tttcgccaac aatgctcgta agggttttta catcgagcgt
     4921 ttgtgccatg gcttcgccaa cgctattgag gatcgctagt tcggcattgc gttcctcggt
     4981 ttccttgagc aggcgctggg tttcgtcaaa gagacgggcg ttttcgaggg cgacactcat
     5041 tgaattggcg agcgtctcaa gcaggcgtac atccgagtcg ctgaacgcat tttcatgctc
     5101 catattgctt aggttaatca aaccacgggc ctgatctcct ataaccagag ggacgaaaac
     5161 cgccgatttc tcaatctctg taccaggcag ggtgaagctg ccatacttat tggtttcttc
     5221 ctcaaggttt tcattaatga ccagtatctt ccgagttcga aggatatgag gagttactcc
     5281 tttatcggtc aattcaattg gatcaacttc gatttgcctt ccgccctcgt aaaaatacgg
     5341 aaaatgaacc agattggtgt ttggatccaa aatacgaata ttcaaatctg tattatggaa
     5401 gatactgcgg attttgtctc ctacagcatc ataaatgcct tggatattaa gttcggccgc
     5461 caaggcagcc tgtacactgt taataatagc taattcagca ttgcgctgct cggtttcctt
     5521 gagcaggcgc tgggtttcgt caaagagacg ggcgttttca agggcgacac tcatcgagtt
     5581 ggcgagtgtt tgcaggaggc gaacatcaga ttcagtaaaa gcattctcct tgtcgtcatc
     5641 ttgtagccca atgttgccga tcacaatatc accgatgatc aggggtatcc agacaataga
     5701 catttttgga tcgtcgagcg tacctaattc aactccaagc tgacgcatag tttcggcgac
     5761 gttcttgtta accaggaaag gttggcgtgt cgttatcagc cgttcatcaa ttttgttgaa
     5821 aggctcaggg tttttgcgca ccggttcgcc actttcccaa gttaaatgca gggtggatgt
     5881 tttggcgaca tggtcgtagg tttcgatggc aacggtattt acattgaatg tttcgcgaat
     5941 ttttgcgcta accaaatcat atatcgcctg aatatttact ttggcaacca gccctgcttg
     6001 tacgctatta atgattgcca gttcggtgtt cctttgctca gtctctttga gcaggcgttg
     6061 tgtctcatcg aagagacggg cgttctcaat ggcaactccc attgaattcg ccagggtttg
     6121 caaaagccga atatcggagg ctgaaaaggc attttctcgg tcgatatttt gcaatgcaat
     6181 aatgcctcgc acaacagttc ccgctttgaa tggcatccaa acccccgatc gggcgagttc
     6241 cccagctgct accgtcatgc gatattcttt tacgcgttcc agcatattct cgttaatgac
     6301 gaccatttcg ccggtctggg caatatgctc cattaatcca ctaaaaggaa tagtttcagg
     6361 gaaatagcgt tgaccttttt catagaagta atcgaatttt gtctgtttgt tttcataatc
     6421 aagggttccg ataatcacgc cctgggcatc aaagatatcc cgaatcttgt cgccaactgc
     6481 atcataaatt ccctgcatat ccagctctgc cgcaagcgcc agttgcacac tgttgatgac
     6541 cgcgagttct gcgttgcgtt cctctgttat ttttaagagg cgttgagttt catcaaataa
     6601 acgcgcgttc tcaagcgcgg tgcccagcga ggcagcgatg gtggttagca gtcgtaactc
     6661 cgagtcgccg aaggcatttt cgcgctcgta gttttcaatg ctaattacgc caaggactcg
     6721 atcacttcca atgattgggg cggtaatcat tgatttgcct gaatctgtac cctggattgc
     6781 tgccaaacct tttttcaaaa aatcttcggg tgtattaagt attagaggaa gccgggttcg
     6841 gcgcatttcc tcaaatggac cctctgctct cggctgaata ggctcaagtt tcaagcgttc
     6901 gccatgttca tattcgtaga gaaagtggat cagattcgct tcttcatcat accaacggat
     6961 tgccatatca gatgtgttga acacctcacg cagtttatca ccgaccaaat ccacaataga
     7021 ctggaaatcc aactcggcgg cgagtccttg ctggatcgag ttgatgattt gtaattcggt
     7081 aacacgttcc tgttcggctt tgaagaggcg ctgggtctca tcgaacaggc gcgcgttctc
     7141 cagtgcgact cccatactgg ccgagactgt ggtgagcagg cggacatctg aattccgaaa
     7201 aacgttttcg tttacatcct gtaaggtgat cgtgccaatc acgatgttgc cactcacaat
     7261 tggaacgccg agccaggatt tcttcgggtt ctcatttggc aggtagaccg cgcccagttc
     7321 cttgaagcgt tgctctgcat tttcattaat caggagtggc tggcgagtat taacaacatg
     7381 ggaacttaat cccgttccta attgataagg gtccggatga gtgcgaatcc cattctccag
     7441 gtaataggga aagtgaatcc attgcgtttt ttgatcatag agggaaatga agatcgtatc
     7501 tacagcgaac agattgcgaa tctcttcacc aaccaggtca ataatagcgt ggaagtccaa
     7561 ttcggcggcg agtccctgct gtatggagtt gatgatctga agctcggcaa cgcgctcctg
     7621 ctcggctttg aagaggcgct gggtctcgtc gaagaggcgg gcgttctcga gggcaacgct
     7681 catcgaattg gtaagcgtac ttaataggcg agcgtcagac tcatcaaagg caaatttttt
     7741 gtaactctga acggccacca cgccttttac ttcactgcca agcatgagag ggacaccgag
     7801 ataggattca ttgaggtcct gccccgtatc agggcgggga gtgggtatgc ctcccaactg
     7861 ggcgccatct tcaatcgtat ttacaatgag cggcttctgc gcccggatca cgtgtgtgta
     7921 gaatccgtgt cccatctgga atggtctgtc gaacttcaaa cggaaaccac gatctattgc
     7981 gtattgaggg tgaaccagtt gatttgcgtg atcgtacaga cctatatagt ttgagtcggc
     8041 attgaatact tcacggattt tttcgccaac gagttcataa atggactgca tatccaattc
     8101 agctgccaac ccctcctgca cactgttgat gatggcaagt tcagcagagc gttcctgctc
     8161 agctttgaag aggcgcgcgc tttcgagtgc cacgctcatc gaattggcga gggtggtcag
     8221 caggcggata tccgaatcgc taaaggcgtt ttccctatcc acgttctgca ggctgacgtg
     8281 tcctgtcacg gttccagata cggataacgg aatccataca cctgattttg gaacacccga
     8341 taagtgcccc gccgaatcat ccatgggttg cgccttatgt cgtactcgct cagccagttt
     8401 ttcctgaata atgatcggtt ttccggtcgc tatcaattcc cggtacaagt cggtggtggg
     8461 ctgggaaccg taggtatgcc gcttgccgtt ttcaatggca tagtgaatga ggtttttccc
     8521 ggccgcgcgg tcataactga ctagggtcac gatctgcgcg tcgaagatgt cgcggatttt
     8581 gtcgccgacc aaatcataaa tggcttgtac atccaatttg gatgccaatc cctgttggat
     8641 ggaattgatg atctgcaatt catcttctcg ttgggcgagt tgttgtttaa gctcggatga
     8701 ttttttctgt gaagaatcct tcaacccact cgctttggaa gtctccgatt ttatccccac
     8761 cctgcgtttc gatttgataa ctttgctcaa agacgatgac tttcctgcca tacccgttac
     8821 tcctcgaatc ctttggttag ctaatcatac tccagcctcg tgctgatgcg ggttcttctt
     8881 tcatatcagg ctgatctggt gccacatgac cgttggattt ttcgcggcgc ttcagcaaaa
     8941 cgagcgtgct tccagttgaa gaaataaatt gacaggccca taacaattga aagatgattt
     9001 gcctaagatc gacaacattt gtggctttcg ttggtttcat cccgccattt tctttgctcc
     9061 tccaaaaccc cttcgtggat gcagagagtt gtatcaaccc ttcagcatga tcgctaaatt
     9121 ctgaatcggc gtggacggaa cgagatcaca gcctggccag atcgcatcta cacctgcttc
     9181 tttcgcgact cgaattgcgt ccgttatcga gacctcgtct ccctgccaca agaccttgac
     9241 cggatctata tttccaaaca gcaaggtgtc tctcaaagct tcacgcgaag cagacaaatc
     9301 atttacttga tcgacagaaa ttgcattggc acccgtttgc gccagcaggt acatagactt
     9361 attcgtattt ccacagacgg agagcacgca gggcttttgc aaattttcga tcagctgctt
     9421 ttcaggtgga aaggcaaatt gttcatactt tgtgggtcca ataaaccccg gcgacccgcc
     9481 catatcatgg atcgtaatgt aatctgcacc tgcgtctgtg tatgccttgc cgatttcggc
     9541 aaggaaagct gaaagatgaa cgagggcatc gagaactgcg ttcggttcgc gcttcatttc
     9601 ggcgaacaag ccgccgggtt caatgaggta taagagcagg gtataaggac ctggaatgat
     9661 cccgctaatg acgacttccc cgccaacctc ttcctttaat agtcgaatcg catcacaaat
     9721 aagtggaatc ctccctttgt tgacgaaatc agcattttca aaataagccg aattcaaata
     9781 ttttgttgat gcaaacagtg gtttcgcagg ttgtgggaat tgatccttct cttgctcata
     9841 ataatgtagg gtagacccta atgcctcagc cggtgcgcac agatccaaag gcaaagccgc
     9901 ggacggcatt cccgtcaaac ggaatgtact cgccgcggct ctcgccattc gttttgcatc
     9961 tttatggact tcgtggagga ctaatccatc accttccagc ccctccgttg tgatatggat
    10021 caacccgcta aaaacaggct ggttggttct cgtattgcct gaaagtaaac caagaatatc
    10081 ttcgcgctgg ctcatatttt atacaatatg agttcttatt cactggttac tggctgcctc
    10141 ttcactgctt ttacaatcgc cgcgatgtgc tcaggtgtat ttccacagca tccgcccacg
    10201 acgcgtgcac ccagttcaac atatctctgt gaaaactttg ccatatcgtc cggacccata
    10261 tcgtacacgc cctgctcggt ctcgatatcc atccgcggaa caccagcgtt cggcttaacc
    10321 cataatggga agtttgccac ggtctcagag tattccttga ccactgcttc catgttttca
    10381 agggttgtcc cgcaattcgc gccgaccagc gtggcaccca tttcactata tttcttgata
    10441 acgtctttcg gtttcacacc catcatggag cgcgtgccgc gatcatagct gaaagaaata
    10501 acaatcggca aatcggtcgc tgatcttgca ccttcaaacg cagccgtggt ctcttcaaaa
    10561 gcaaacatcg tttcaattaa taataggtca acaccgccat cggcgagcgc ttttgcctgt
    10621 tctgcaaacg tcgctttaac ttcctccgcc tccagtggcc cgtatggctt gatcaataat
    10681 ccaaccggac ccatggaacc cgccaccagg accccctcac gcgccgaggc ggctttgcgt
    10741 gcgatctcca ccgcgcgaat attcacttca ggggttcgat cctgatattt tgaatccttc
    10801 atccgcatac gtgtaccgcc gaaggtgcaa gtgaggacga tgtctgaccc tgcctcaaca
    10861 aaggaacttg ccagtttcaa aatcgtgtcg ggcgcatcaa taataagatc ctcaggcggc
    10921 ttgccaggct tgagccccat cttttgcagg ttggaaccag tcgctccatc tgcaactaag
    10981 atttcatctg aattcaatcg ttctaagaat ttgttcatta tatgtgtcct ctggtggtcg
    11041 agtagatacg agcgatagcg agtgtcatat cgagaccacc aataaccaaa atcattttgc
    11101 tcatgtggtg gtctcgatac gggctacgcc cactcgacca ccagaatatc atttcataaa
    11161 attcttcgcc aacgggtcca gaacgcgatt cggtattagg tgtgcaatgt gttcaaacgg
    11221 ctcgctcatg tgcgggaaac ttgtcgccag catgaattga ttctgaaact ccgggtcggt
    11281 tggcaattca aatcgttcca ccttgcgcgt tacagaatca atctcttttc gtttttcaac
    11341 attcaacaac gcgatgcggg cgccgtcacc tgcggcattc ccaactgcat acacattctc
    11401 aagtttgcaa tcggggatca aaccaatgag catggctttt tctttatcaa tataacttcc
    11461 gaagccgcca gccagaataa ttttatcagg gctttgcaaa ttgctgcgct ttaataatgt
    11521 acgcgccgcg gtaaacaagg cagcttttgc caattggatt tgacgtacat cctgctgggt
    11581 catggggatg tctcttccga tggaggtctc atccgcccat gcaacgacat actcccagcc
    11641 gttttcgcct tctcgaatcc gcttcgaatc taaattcttt ttgaacttgc cgcgagaatc
    11701 gacaatcccc gcgcgaaaca gttctgccac cgaatcgatg atggcggagc cacaaatccc
    11761 tttgacatgc cctttgaatt cggcatggtc cgtgttccag ccttccacgc caataacttt
    11821 gtatttcggc tcaagcgtcg cttcatcaat atggactcgt tcaattgcac caggggcggc
    11881 acgcattccg tactccacgt gggcgccttc aagcgcaggg ccggtcggtg tggatgtgca
    11941 aacgagtcgt ttgcgattcc ccaggatcaa ttccgcgttt gtgccgacgt cgatcagcag
    12001 ccagttttca tcctgcttgt gaggttcctc tgcaacgatc atggcgctcg tgtccgcgcc
    12061 tacaaaggat gcaatagtgg gcaagacgtg aatatttccg gacgggttga tttgcaagcc
    12121 aagttcccgc gcttttacat cgactgattt atgaatcgcc ggcacgaacg gcgcgagtcc
    12181 aagatctttg gggtgcaaat ttagtaaaag atgatgcatg gttgaattgc cgaccagcac
    12241 catttccagg atttcgtcag ttttgatgaa ggcagtttgc gcagcctgct tgagtaattt
    12301 attgagcgtt tgaataacag ctttatgcag tttttccaaa ccgtcggcat gttcgatcgt
    12361 gtactgaatg cgcgacatca catcttcgcc atacacaatc tgcggattca tttctgattc
    12421 agccgcaagt aattctcctg ttcgcaaatt gcacaaatac aacgcgaccg ttgttgaacc
    12481 gatatcaact gccgcgccgt agctatcctc cacatatcca gcctggactt caatcacttc
    12541 cttatcctgc cagacggaga cggtcacatt ccactttgcg gcccgtaagg tggaagaaag
    12601 tgtgcgtaag cattgataat cgatatccag gtcgaaccag cgcggcagat tctcttcacc
    12661 gcgtcggacc agtcccatgg atgtttcaag tcccttggcg agtcgctccc agtctgcgat
    12721 cggacgctcg agattgggcg gcgacatgga aacataatac ttacggacag agggcttgat
    12781 ctcaatcgcg cgctctcgcg ccgacttgcg gacgatctgt ttattgccgc ggctctcttc
    12841 gggcacattg atcagcacat cgcccagcac tttgcactga cacgataaac ggacctgccc
    12901 gatttcccag cctttatcct ttaataactt tggtctgcgc atcaaatagg cgcgctcttc
    12961 tgtgccaaca ggggagaggt gctcacgcct cgagtcgatg ttgtactttt cgaagcgacc
    13021 ttcttctatt aataccatgc actttccgca ggtcgcgttc tctgcacaaa tggactcaat
    13081 ctccacgccg agttcacgtg ccgcagctcg aatggaggtg ccttcgtcaa cctgtccgcg
    13141 gcggccagag ggttgaagaa tgatcgtgtg ttttttggtc ataaggtcac tcgtagggaa
    13201 cggcgccctc atcagggtgc ttcgcgagct gccgttctct actatgtatt acgcgtgatt
    13261 tccgtttcgc ttgggaatcg gtagttttgc gaactcagtt tccaattcca ggagaagagc
    13321 ttctgcaact tcttccggtg ccccgtcaca ttctatccat tctcctcgtt tatattgtgc
    13381 agaatagtca cttgttgagg ttaatccctc tgccattgca tatgcgtcaa tcttgttttg
    13441 aatcttctca gaaagttgtt tctttacagt tcgcccatcg ccttcgacgg tgaacgattg
    13501 aggaatgtgc ttccaataca taatacgata tctagccatt tgaacccttc caaaattata
    13561 tataatctgt ttattctaag ggaggttagt aacaaggtca atagcatgga tgaatggcac
    13621 cggcactgtg agtttgtaac ctttcaattc tgaaagcgtc ttgtccgcaa ctagtacgtt
    13681 aagtcactat tcgtttttat catcagtagt gggggcaaat tggcccgagc ctaacacatt
    13741 ttgccgggat tatcataaaa aagttggtta cattgaatct tccgcgttac tttattaata
    13801 gaagaggaga gtacgatgtc agatatgggc aaatttaggg gcacatcttt acgtttgtgg
    13861 tttatgatcg cgctggtgat tggatcagca atacccgtac atataagggc ctctgcccag
    13921 cagtctcccg caccgctttg ggtcgttcaa tcgctttcga ccagtgagta tggcgtgatt
    13981 gctccgcagg gattggcctt ttcagcagat gaaaaagcat tcctggtgtt ggatggaacc
    14041 tctgatgtga ccttgatcac tatagcagat gagccagccg gcaaaaaggc aatcgcagac
    14101 gtgggttccg atcctttgaa cacagcgttt gacaaacgca cgaatagcct ggttgtgctt
    14161 gatcgcggcc gaggggagtt ggcaaaggta aaagaagatg gttctagtat gcgctttgca
    14221 atcggtgggt tgaagctggc cgatccgcaa ggcatcacta ttaaccctgc gaatggtcag
    14281 atgtttgtcc tcgatgcagg caaagcctat attgtgtcag taccaacgca tgatactctt
    14341 ggcgttgatg ccagcaaaat gcagcagatc tcgctgcagg ggctgggcgc cggtaagttc
    14401 aaaggtctgg cgtacaaccc taacaatgga catttgtata tttccgaaac cacgcaaaag
    14461 aaattatatg aaatcaccca aagcggcgag ctggtcaaca catttgattt gactgcggtg
    14521 gaaattagca atccgtcggc catgactttt gctccgagtg tggataatac cgacgatcca
    14581 aacatctatg atctgttcat cttggatacc acttccagcg gtcagatcgt tgaattgtca
    14641 ttacagcagc ctgcggcgtt gccttctggg acaacgttgc tgccagcaaa catggtgaac
    14701 attatcgaca cttccaatca ggcgtggaat ccctccgcgc cggacccggc gggccttgat
    14761 tattggccgg ctgccaatcg tctggtcatc agcgatagtg aagtggatga gatgccgcca
    14821 tacttccagg gtaaaaatgt atttctctcc acaacaagcg gtagcctgac cggaacttgt
    14881 tcaacaacgg cgtataccgg cgagcctacg ggagtaggag tcaacccgaa caacaaccac
    14941 atcttcatcg cggcggattt tcaggatcgt ctatttgaga ttagcctggg accagatggg
    15001 cagtactgca cctcggatga cacgtttaca aacacaagcc tggccacagc ctatggtgtt
    15061 acagatgcag aggatgttgc ttacgggaac aacacggtat tcattgctgg aggggcggat
    15121 gcagaggtat ttgtgattcc gctcggggcg aatggcgtgg ttggcggtgg ggatgatggt
    15181 ccaatgaccc attgggacac tgctgccctg ggcttcgcag atgccgaagg aattggtttc
    15241 aatcatgatg ctggcacgct ctttgtcgtg agcacgaagc gcactgacaa atatctcggg
    15301 gagttttcta ccacgggcac gctgctgcga gcctataatc tatcgttcat gcctgatgta
    15361 ggcaacattc gctcggatgt cacttatgcg ccgggtagtc agaatcccct ggtaaaaaac
    15421 atttacatcg ccagccgtgg cattgataac gacagcaatc ccaatgagaa tgatggaaag
    15481 atctgggaga tcaacatcgg gagcgcaccg agcgccacaa atactcctgg accctcacct
    15541 acgtttacga atacggcaac tcaaacatcc acaccgacga ttactcccac atctacagcc
    15601 atcgtccctc cgtcagggaa ttcattccat acctcctttg gaagtggcgg tacgttaagt
    15661 ggggtagctt ttgctgatga tgatattatg cacttcgacg gcagcacctg gagcctgtac
    15721 tttgatggtt cagatgttgg actttcgtca gtcgatatcc tggcgatcca aagcctggat
    15781 gctgattcac tcctcttgtc cttcaacact gcgatcacac tcaatggaat agcatttgcg
    15841 tccacagatg tcgccaggtt cgatgcaact tcactcggca gcaacaccgc cggaacgttc
    15901 agtatgtatt tcaatggtgc agatgttgac ttgagtgcat ccggtgaaaa tattgatgcg
    15961 tttgagattc ttccggacgg taagttattg gtctcgacga ccggcaatcc aacagtcact
    16021 ggtgttagcg gtgcacgaga tgaagatatt cttcagttta cacctacaac actggggacg
    16081 aacacaagcg gaacgtggtc tttgtacttc gacg
//
LOCUS       DASTJP010000706         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1540244, whole genome shotgun sequence.
ACCESSION   DASTJP010000706 DASTJP010000000
VERSION     DASTJP010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540244"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFQ23_20615"
     CDS             complement(<1..273)
                     /locus_tag="VFQ23_20615"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9909063.1"
                     /translation="MRFKKLNYIRLLIIFLITSGCAATRPEAGETRVSEKDGMVQVYI
                     PAGAFTMGSSLDDPTADEDERPAHHVLLDSFWMDRTEVTNAMYLRCI"
     gene            complement(362..1690)
                     /locus_tag="VFQ23_20620"
     CDS             complement(362..1690)
                     /locus_tag="VFQ23_20620"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9909064.1"
                     /translation="MKRLYTLVSVLMLATLVLAACGGGATTEAPATEMPATEAPMTEA
                     PMTEAPMTEAPAAFECTDAIGCVDIAPGEPIHIAWIQSVSGATAALGQTNVNGGQIAI
                     DDRGGELLGHPIQYDGEDSLCNAEGGQAAGTKIASDPTVVAILGTTCSSEARAASPLI
                     SDAGMVMMSSSNTNPDLTNPDHPDFHTGYFRTAHNDLFQGRIAAEYAFNELGFTTAAT
                     VHDGSPYADGLRKVFEAVFAELGGTITSSEAVNVGDTDFKPVLTTIAASAPEIIYFPI
                     FEPEGNLLASQKCEVSGLEDTALMGADGLFTSGFASTSGSCAVGMYLSSPYVAGDAGA
                     QFVEKYNAAFGEDPASGFGPHSYDALNIFFAAIEQVAVVDADGTVHIPRQALRDAVYG
                     TTGFDGLTGSLACDENGDCATGEALAVYQISQEMVDGSVNLTTSVPVWQP"
     gene            complement(1936..2475)
                     /locus_tag="VFQ23_20625"
     CDS             complement(1936..2475)
                     /locus_tag="VFQ23_20625"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9909065.1"
                     /translation="MLHYLNLEVAVSTASIVNFLKQSDIFFQFTPTQLELVANLCQEA
                     VYQKDDLIFKENSGSKELYVIVQGEVDIFVDPSLVGTLEAGSENRTIATLRRGQSFGE
                     VALVDEGLRSASACASQKDTRLLIIPRDKLIMLCDTYPQLGYRLMYNLAADLAMKIRN
                     TDLRIREQLLYKPPERTNK"
     gene            complement(2496..>3683)
                     /locus_tag="VFQ23_20630"
     CDS             complement(2496..>3683)
                     /locus_tag="VFQ23_20630"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HET9909066.1"
                     /translation="QLVIPGLEGVNGILETEVISFGDTLRSLSRRTQVSDEQMKKLNR
                     LISPTELYVGVSLIVPLQEGQTPLTTRMTPSNGESLLELAVKQGSDPWTLTTLNKISG
                     TWDALPGDILYSPSDNGVSSATGLPSAFVSASIEPLPIVQGSTEVLEVMLQGNATISG
                     TLVDKPLNFFDANGSQVALQGVHALLEPGVYPLSLEVTLADGSKQSFEQMVLVNSGNY
                     LSEAIPINDPSTLDPAVTEPEISNITAITTPATLTRYWNGLFTTPSVYTDCFTSRYGT
                     RRLYTVINSDIEIPGFHSGLDFCGGEDLQIFAPAPGKVVFAAPLTVRGNATIIDHGWG
                     VYSGFWHQSETLVNVGDVVEQGQVIGLVGGTGRVTGAHLHWEVWVNGVQVNPLEWLNQ
                     AYP"
BASE COUNT          924 a          926 c          947 g          886 t
ORIGIN      
        1 aatacaacga agatacattg cgtttgtcac ctccgtgcga tccatccaaa atgaatccaa
       61 taatacgtga tgagcgggtc tctcgtcttc atcggctgtt ggatcatcca gggagcttcc
      121 catcgtgaat gcacctgccg gtatatagac ttgaaccatg ccatcttttt cagatacacg
      181 agtctcgccg gcttcaggtc gggtggctgc acacccgctg gtaatcaaaa agataataag
      241 aagccttatg tagttaagtt tcttaaatcg cattctatta gggatgagaa atgggtcggg
      301 caataaattc tgcccgaccc aaattttgta cgtttatctt aggttcttga aaacttcaga
      361 cttatggctg ccagacagga accgacgtgg tgaggttcac gctgccatca accatttctt
      421 gcgaaatctg ataaacagcc agggcttcgc ctgttgcgca gtcaccgttt tcatcgcagg
      481 caagcgagcc ggttaagcca tcaaagcctg tggtgccata tacagcatcg cgcagagcct
      541 ggcgaggaat atggacggta ccgtcggcgt cgacgacagc tacctgttca atggcagcga
      601 agaagatgtt cagcgcatca taagaatgcg gaccaaagcc agaagcagga tcttcaccga
      661 aggcggcgtt gtacttctca acgaattgcg cgccagcgtc accggcgacg tacgggctgg
      721 agaggtacat accgacggcg caggaaccgg aagtgctggc aaaaccagat gtgaacaaac
      781 cgtccgcacc catcagggca gtgtcctcaa gaccggaaac ttcacacttc tgggaagcca
      841 gcaggttgcc ttcgggctcg aagatcggga agtaaatgat ctcgggagcg ctggcggcga
      901 tggttgtcaa cacaggtttg aaatcggtat cgccaacatt gaccgcttcc gatgaggtga
      961 tggtgccgcc gagttcggcg aacacagctt cgaagacctt gcgaagtcca tcagcataag
     1021 gggagccatc atgaacagtt gcggcagtgg tgaagcccag ttcgttaaag gcatactcag
     1081 ctgcaatacg accctggaag aggtcattgt gcgcggtgcg gaagtaaccg gtatggaaat
     1141 ctggatgatc agggttggtc aaatccggat tggtgttgga ggatgacatc atgaccatac
     1201 cagcatcaga aatcaatggg gaagcagcgc gcgcttcgct cgagcaggtt gtgccgagga
     1261 ttgcgacaac ggtgggatct gaagcaatct tggttccggc agcctgacca ccttcagcat
     1321 tacaaaggga gtcttcgccg tcgtactgga tgggatgacc gagcaattcg ccaccgcggt
     1381 catcgatcgc gatctgacca ccgttaacat tggtctgacc caaagcagca gttgcaccag
     1441 atacggactg aatccaggcg atgtgaatcg gttcacccgg ggcaatatcc acgcagccga
     1501 ttgcatcggt acattcaaaa gcagcaggtg cttcggtcat gggtgcttcg gtcatgggcg
     1561 cctcagtcat gggcgcttca gtggccggca tctcagtcgc gggggcttca gttgttgcac
     1621 caccaccaca ggcagcaagt accagggtag caagcatcaa cactgaaacc agtgtataca
     1681 agcgtttcat ttctttctcc tccgaaaaat tgttagttgg aaattgtatc cttatggttt
     1741 ggatacgacg atgatcgggc ggacaatgct cttctctgtc agcatcacct cctttgaaat
     1801 caaaactctt ctgaaattca gactaacgcc gcagaaactt ctcacggctt tcgaccattc
     1861 ttgatgtgag gcacgcgcaa ttataagggt taatagtaag gcaaatatct taatgggtca
     1921 agtgcttgtt tggaattact tattcgttct ttccggtggt ttgtataata gctgctcacg
     1981 aattcttaaa tccgtatttc tgattttcat ggcaagatcc gctgcaaggt tatacattaa
     2041 ccgataccct agttgtggat acgtatcaca aagcatgatc agtttgtcac gcgggatgat
     2101 caaaaggcga gtgtcttttt gtgaagcgca ggcagacgca gatcgcagcc cttcgtctac
     2161 cagcgctacc tcaccgaagg attgacctcg ccgtagcgtt gcgatggttc tgttttcact
     2221 gccggcttcg agcgtgccta ccagcgacgg gtcgacaaag atatccacct caccctgaac
     2281 gatgacgtac agttccttgc tcccactatt ttccttgaag atcaaatcgt ccttctgata
     2341 cacagcttcc tgacaaagat ttgccacgag ttccagctga gtaggtgtga actgaaaaaa
     2401 gatatcgctt tgttttagga agttgactat cgacgcagtg gacacggcga cctccaggtt
     2461 aaggtagtgt agcataaaga agtggggagt gcaatctacg ggtatgcctg attcaaccat
     2521 tccagcggat tcacctgcac gccgtttacc catacctccc agtgcaggtg cgcacccgtc
     2581 acacgtcccg tgccgcccac caaaccgatc acctgacctt gctccacgac atcacccaca
     2641 ttgactaacg tttcagactg atgccaaaat ccgctgtaca ccccccagcc atgatcgatg
     2701 attgtggcat tgcctcgaac agttagcggc gcagcaaaca cgactttccc cggtgctggc
     2761 gcaaagatct gcaggtcttc accgccacaa aaatccaacc ctgagtggaa gcctggaatt
     2821 tcaatatcgg aattgattac tgtatacagc cgtcgagtgc cgtagcgtga ggtaaaacaa
     2881 tccgtataaa cggaaggtgt cgtgaagagc ccgttccaat agcgggtgag agtagcaggc
     2941 gttgtaatcg cagtgatatt gctaatttct ggttcggtga cagccggatc aagtgtactt
     3001 ggatcattaa tgggaatagc ttcacttaag tagtttcccg aatttacaag caccatttgc
     3061 tcgaaagatt gtttgctccc atctgcaagc gttacttcca ggctcagtgg gtaaacacca
     3121 ggttccagta aagcgtgaac cccttgtaac gcgacttgac tgccatttgc atcaaaaaaa
     3181 ttcaaaggtt tatctacaag cgtcccggag atagttgcat ttccctgaag catcacttcc
     3241 aagacttcgg tgctgccctg tacgataggc aatggttcaa tgctcgcgct tacgaatgcg
     3301 gatggtaacc ctgttgcgct acttacacca ttgtcagagg gggagtagag gatatcaccc
     3361 gggagcgcat cccatgtgcc gctgatctta ttcagagttg taagtgtcca ggggtcgctg
     3421 ccttgcttga cggcaagttc gagcagagat tcgccgttgg acggagtcat tcgggtggta
     3481 agcggagttt gcccttcttg aagaggcaca atgaggctga cacccacata aagttctgtg
     3541 ggactgatca ggcgattgag tttcttcatt tgctcgtctg aaacttgcgt gcgcctgctc
     3601 aggctgcgga gagtgtcacc aaaggaaatg acttctgttt caagaatacc gttcacaccc
     3661 tccagaccag ggatgaccag ttg
//
LOCUS       DASTJP010000707         4304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_762805, whole genome shotgun sequence.
ACCESSION   DASTJP010000707 DASTJP010000000
VERSION     DASTJP010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762805"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFQ23_20635"
     CDS             <1..473
                     /locus_tag="VFQ23_20635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9909067.1"
                     /translation="GTGKNIEFWPANAEITAIDLTPGMLAFARQRAMSLDSDADLRLS
                     DVQFLEFPSASFDSVVATFVFCSVPDPVQGLREIGRVTRPGGQILLLEHVRIERPIIG
                     KMMDALAPLIVRLNGANINRRTIKNIRIAGLQIEQVEDLDSMGMFKLILARAHA"
     gene            512..736
                     /locus_tag="VFQ23_20640"
     CDS             512..736
                     /locus_tag="VFQ23_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909068.1"
                     /translation="MEHEQVEKQVARQSALIYVPLSLGSALLFLLVSTFTGNYPLVAR
                     VGGTVWVALLSLIVSMPVVISKVKKDSRNS"
     gene            767..1801
                     /locus_tag="VFQ23_20645"
     CDS             767..1801
                     /locus_tag="VFQ23_20645"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9909069.1"
                     /translation="MYLGFSRRDFLKASGAGLLGLFLADLRLERVFASPTPKQGRVID
                     SGVELFSEPLFSSKITHLFGRDQVVEITGEVQGDQGYGNPFNTAWYQVNNEGFVYSGR
                     IQPVETKHRMPVFNIPAEGALGEITVPFCDTKRAISTYADRGYRIYYGTTHWITETLV
                     NRAEKSIWYKIFDGQLKESFYVPAHEMRVIPPGELALLSAEVPDEAKRVYIDLASQTV
                     TAFEGENPVFVTRCASGAKGTDTPLGDFRTYHKGPSVHMTNQGDGTENTYHLPGVPWV
                     SFFTGSGVALHGTYWHNDYGRPSSRGCVNLTPSDSKFFYLWTRPNVPPETPYLFRPGE
                     GTLVQVVSSI"
     gene            1828..2070
                     /locus_tag="VFQ23_20650"
     CDS             1828..2070
                     /locus_tag="VFQ23_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909070.1"
                     /translation="MSRIKVSKTSPGSTELTQQKKEWPVVVYLWGFGLAILGYAVGRV
                     GLASLPHPYHWMSGVVGAVVGILIGWLWYRWRGDVF"
     gene            2197..2448
                     /locus_tag="VFQ23_20655"
     CDS             2197..2448
                     /locus_tag="VFQ23_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HET9909071.1"
                     /translation="MMGGGMMGMHGFGGMGLFGGWIGLLFNLAIIIGIVILVVWAVKR
                     FTSGSVSSGSQTPREILQARYARGEITRDQYQQILLDLN"
     gene            complement(2549..3826)
                     /locus_tag="VFQ23_20660"
     CDS             complement(2549..3826)
                     /locus_tag="VFQ23_20660"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET9909072.1"
                     /translation="MKRIKSPRLYLIILVAMLAGILTAYRLPSFQERVDWRINDLVGR
                     VKYALSPPEQVVFIPQEKVVVPSRIAPSKSPTLSSVPTSTIAVTETPFSAPSQTATPI
                     PDRVTLTGFTHEYQTWNNCGPATLGIALSFWGWEGDQQPIAAFTKPNSRDKNVMPSEL
                     VAFVEEETEFDVVSRAGGNVELLKRFLASGMPVIIEKGFEGPKLEGWMGHYVLVTGYS
                     DAEERFTVQDSFFGPDQGMSYADLESYWRAFNFAFLVVYPADRKDDVENLLGPLVDEE
                     YSYRSAGETASNEIYLLTGRDQFFAWFNRGTNLMRLQDYAGASQAYDQAFQLYPSLPE
                     KGRPWRMMWYQTGPYWAYYYTGRYQDVINLATQTLDAMSQPVLEESYYWRALALLAWG
                     DSKGAIKDLRSSLEYHPGFEPSITRLQQLESTP"
     gene            complement(3823..3984)
                     /locus_tag="VFQ23_20665"
     CDS             complement(3823..3984)
                     /locus_tag="VFQ23_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909073.1"
                     /translation="MALLCQIMGKPDLQLTFQSVGGIPNVPWQSGLSTPMKQAQNISF
                     GFYGEALLE"
     gene            complement(4003..>4304)
                     /locus_tag="VFQ23_20670"
     CDS             complement(4003..>4304)
                     /locus_tag="VFQ23_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909074.1"
                     /translation="NLHWSPTGGPFTRNIQKLCPDATSDLEACDVRIHFDGTVAALQW
                     IDGNRILFLTRNPSVLFLGEMDFTGNMDGTTIPIVAWPLEDWVSATSFTMAEVSQ"
BASE COUNT         1056 a         1057 c         1124 g         1067 t
ORIGIN      
        1 tgggtactgg aaagaatatt gaattttggc cggcaaatgc tgaaattaca gcgattgatc
       61 tcacacctgg gatgctggct tttgctcgtc agcgcgcgat gagtctagac tcggatgcgg
      121 atttgcgcct gagtgacgtg caattccttg aattcccctc cgcaagtttt gatagtgtgg
      181 tggccacgtt cgtcttttgc tctgtccccg atccagtcca gggcctgcgc gaaatcggcc
      241 gcgtgactcg cccaggcggg caaatcctgc tgctggaaca tgttcggatt gaacgaccca
      301 ttattggcaa aatgatggat gccctggctc cgcttattgt tcggctgaat ggtgccaaca
      361 ttaaccgcag aaccattaag aatatccgaa ttgcaggact gcagatcgag caagtcgagg
      421 atctcgatag catgggcatg ttcaagctta tccttgcacg agcgcacgcc taatccgaga
      481 tattacagag tttgtttaca aggagtatct tatggaacac gaacaagttg aaaaacaagt
      541 cgcgcggcag agcgcgctga tctatgtccc tctttcactc ggatcggcac tcttgtttct
      601 gttggtaagt acatttacag gcaattaccc gctggtggct cgcgtaggag gcacggtctg
      661 ggtagccttg ctaagcctga tcgtatctat gcccgtcgtg atatcaaagg ttaagaaaga
      721 ctcacggaac tcctaggtac actccttttt gctactgagt tactccatgt atctaggttt
      781 ttcccgccgg gacttcttga aggcctcagg cgcaggactg ttaggactgt ttctggcaga
      841 cctccgcctc gaacgggtgt ttgcgtctcc gacaccgaaa caggggcgtg tgatcgacag
      901 tggtgtcgag ctttttagcg agccgttgtt cagttctaaa atcacccatc tgtttggacg
      961 tgatcaggtc gttgaaataa caggagaggt tcaaggtgat caagggtatg gcaatccgtt
     1021 caacacggcg tggtatcagg taaataacga aggcttcgtc tactccggtc ggattcagcc
     1081 agtagaaaca aagcaccgaa tgcccgtctt caacattcct gcggaaggcg ctcttgggga
     1141 gatcacggtt cccttctgcg ataccaagcg tgcaatcagt acatatgcag atcgtgggta
     1201 tcggatctac tatggcacaa cccattggat cacagaaaca ttggttaacc gcgccgagaa
     1261 gagtatctgg tacaagattt ttgacggcca attgaaggaa tcattctacg tgccggctca
     1321 tgaaatgcgt gtgatcccac ccggtgagct ggcactgctc tccgccgaag tgccagatga
     1381 agcaaagcgt gtttatattg acttagctag ccaaaccgta acggcgttcg aaggagaaaa
     1441 tcctgtcttc gttacacgct gtgccagtgg tgccaaaggc acagatactc cattaggtga
     1501 cttccgcacc tatcacaagg ggccgagtgt ccacatgacc aatcaaggcg atggaaccga
     1561 aaacacatac catttgcctg gtgttccctg ggtatccttt tttacgggta gcggtgtcgc
     1621 tcttcatggt acgtactggc acaatgacta tgggcggcca agtagccggg gttgcgtaaa
     1681 cctgacgcca agtgactcaa agttctttta tctatggacc cgaccaaacg ttccaccgga
     1741 gacaccatat ttgtttagac caggagaggg tactctggtc caggttgtat catctattta
     1801 agaagcatct cattgtcagg agaaaatatg agcagaatca aggtatcaaa gacctctcca
     1861 gggtcaacag agctgaccca acaaaagaag gaatggccag ttgttgttta tctctggggg
     1921 tttgggctgg caattctagg ctatgctgtg gggcgcgtgg ggctcgcgag cctccctcat
     1981 ccataccatt ggatgagcgg ggtggttggt gcagtcgttg gaatcctgat tggttggctc
     2041 tggtatcgct ggcgcggcga cgtcttctaa tgccaatgtt tttcttcacc aaatctttac
     2101 actatcttca aagaacgttc aatatcgctt gatagaattc cagcatatgt tcagaacaca
     2161 tcgagatata aagcttgatg gaaaggagaa acaactatga tgggcggtgg catgatgggt
     2221 atgcacggct tcggaggtat gggcctcttc ggcggttgga tcggcctact gtttaacctc
     2281 gcgattatca tcgggattgt gatcctggtg gtttgggcag taaagcgctt taccagtggt
     2341 agcgtttcat ccggcagcca aacgccgcgt gaaattctgc aggcccgtta cgctcgcggg
     2401 gaaattactc gcgatcagta tcagcagatc ttgctggatc tgaattgatt ttttcaaaga
     2461 ggtgcatatc tggtccaaat gagacggctg gccaacgacc agccgttttc tgcttctatg
     2521 aatcggggca acccaatgac aacagagtct agggcgtgga ctccaactgt tgaagcctgg
     2581 tgatagaggg ctcaaatccg gggtgatatt caaggctcga gcgaaggtcc ttgattgcac
     2641 ccttgctatc gccccaagcg agaagcgcca gggcacgcca ataataactt tcttccaaaa
     2701 caggttggct cattgcatcc agtgtttgcg ttgccaggtt gattacatcc tggtaacgtc
     2761 cagtgtaata atacgcccag tagggaccgg tctgatacca catcattctc catggccttc
     2821 ccttctcagg gagggacgga taaagctgga atgcctgatc atacgcctgc gaggctccag
     2881 catagtcttg taagcgcatc aggtttgtcc cacgattgaa ccaggcaaag aactgatcac
     2941 ggcctgtcag aaggtaaatc tcgttcgaag ccgtttcacc agctgatcga tagctatact
     3001 cttcgtccac aagaggtccc aataaatttt caacgtcatc tttgcggtcg gcaggataga
     3061 cgacgaggaa tgcaaaattg aacgcgcgcc agtaggattc taggtccgca tagctcatgc
     3121 cctgatcggg tccaaaaaag ctatcctgca ctgtaaagcg ttcctccgca tcactgtaac
     3181 ccgttaccaa aacataatgg cccatccaac cttcgagctt gggcccttca aaaccctttt
     3241 caataatcac cggcataccg gaagcgagaa accgtttgag taattcgaca ttgccacccg
     3301 cacgcgagac cacatcaaat tccgtttctt cttcgacaaa cgccaccaac tcagatggca
     3361 tgacattctt gtcgcgcgag ttaggcttgg taaacgcagc aataggttgc tgatccccct
     3421 cccagcccca gaaagagagc gctataccca gtgtcgctgg gccacagttg ttccatgtct
     3481 gatattcatg cgtgaaccca gtcagagtta ctcggtcggg aatcggtgtg gctgtttgag
     3541 atggagcaga aaaaggggtt tcggttacgg caattgtgct ggtagggaca gaagataaag
     3601 taggcgattt cgatggcgca attcggctag gtactacaac cttttcttgg ggaataaaga
     3661 ccacctgctc tggaggagat agagcatatt tgacgcgccc cacgagatca ttaattctcc
     3721 aatcaactcg ttcctgaaaa gacggaagtc gataagcggt gagaattccg gcaagcatcg
     3781 ctacaagtat gatcaggtac aagcgtgggg atttgattcg tttcattcaa gtaaggcctc
     3841 gccataaaaa ccaaagctaa tattttgcgc ctgtttcatg ggtgtagaga gcccgctttg
     3901 ccagggtacg ttcgggatcc cgccaacgga ttgaaaggtg agttgtaaat cgggcttgcc
     3961 catgatctgg caaaggagtg ccacatctgc atctactttc tcttactggc tcacctctgc
     4021 catagtgaaa ctcgttgcgc tcacccagtc ttccagcggc caggcgacga tgggtatcgt
     4081 tgtgccatcc atattgcctg tgaaatccat ctcgccaagg aataagaccg acggattgcg
     4141 agtcagaaat aggatgcggt tgccgtcgat ccactggagg gccgcgacag ttccatcgaa
     4201 gtgaatacga acatcacatg cctctaaatc actggtggcg tccggacaca gcttctggat
     4261 gttcctggta aaggggccgc cagtgggtga ccaatgcaga ttgg
//
LOCUS       DASTJP010000708         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_137611, whole genome shotgun sequence.
ACCESSION   DASTJP010000708 DASTJP010000000
VERSION     DASTJP010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137611"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..810
                     /locus_tag="VFQ23_20675"
     CDS             52..810
                     /locus_tag="VFQ23_20675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9909075.1"
                     /translation="MREQLLIGKVALITGSAKGIGRASAIALAEAGADVILGLKDISK
                     SEQVEEEIKQIGRRVLKVQMDISRLDQIQEAVDEGVRHFGHIDILVNNAGIGAPNKAE
                     DVNEQDFDSTINVNLKGTFFTAQAVGREMIKQKSGSIINLSSQAGFIALETESIYCMT
                     KAAISHLTKCLALEWAKHNVTVNAVAPTFIRTPGTAKWLDDKKFHDSVIKRIPIGRVG
                     EPKDIAEIIVFLASPKASLITGATIMADGGWTIQ"
     gene            933..1187
                     /locus_tag="VFQ23_20680"
     CDS             933..1187
                     /locus_tag="VFQ23_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909076.1"
                     /translation="MFTIKLDKINIVLLAIAVLLGAWSIFMPAPAGGFFPLVLVFLAI
                     AYIRTIYMNSGRTAAIVMSAIIVLIPLALYAFYYFLTSSR"
     gene            1258..1689
                     /locus_tag="VFQ23_20685"
     CDS             1258..1689
                     /locus_tag="VFQ23_20685"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9909077.1"
                     /translation="MGLKLFMLGLTVRDMPRALEFYRRLGLDVPEGSETSTHVEIPMG
                     RGFTFFLDSNPGRWDPGFDVSDQPDLPESPQWYPMVLEFYVRNQSALEAKYAELIDFG
                     YESCREPYRTSFGMYFAMVKDPDGNTILISAKAKDNSETEV"
     gene            1728..2099
                     /locus_tag="VFQ23_20690"
     CDS             1728..2099
                     /locus_tag="VFQ23_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909078.1"
                     /translation="MHMTLLSRDSFLVPTPVKLILATGLFFLFGWVIWPVIFTAFMTD
                     WNQVGFPLAIRATGLCAPAFVCIEFRWTALLIDAIFWYLAAAAVVHARVGFWTFCLYF
                     VVTVLGMILLSFLVPLIDIAL"
     gene            2217..>2568
                     /locus_tag="VFQ23_20695"
     CDS             2217..>2568
                     /locus_tag="VFQ23_20695"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9909079.1"
                     /translation="MDKQYEVIIIGGRVAGSSLAIRLAQQNRKVLLIDRATFPSWPAV
                     PSSPLVHPGTMRLLDEVGMSDSEYAYPEGKVTHLILDVMGKYQAIMPLELLQLDRNYV
                     FGIDRNRFDTLLWQR"
BASE COUNT          688 a          561 c          656 g          663 t
ORIGIN      
        1 ttttgaaaca gcggaataac aaagcttgcg cggacgcaac ctcgaggaaa tatgagagaa
       61 caattgttaa tcggaaaagt agcgctgata acaggttctg caaagggaat aggtcgtgcc
      121 agcgcaattg ccctggcaga agcaggcgcc gatgtaatac tgggactcaa ggatatcagc
      181 aaaagcgaac aagtggaaga agaaattaaa cagatcgggc gtagagtact aaaggtccag
      241 atggacattt caagacttga tcaaatacaa gaggcagtgg atgaaggtgt gcgccacttt
      301 ggtcatatcg atattctggt gaataatgca gggatcggtg ctccaaataa ggcagaagat
      361 gtaaatgagc aggatttcga tagcacgatt aatgtcaatc taaaagggac attcttcaca
      421 gctcaagcag tgggccgcga aatgataaag caaaagtcag ggagcataat aaacctcagt
      481 tcacaggcgg gattcattgc gctggagacc gagtcaattt attgcatgac gaaagcggcg
      541 atttcgcatt taacaaaatg tttggcattg gaatgggcaa aacataacgt cacagtaaac
      601 gccgtggcgc ccacgtttat tcggacgccg ggcacggcaa aatggttaga tgataagaaa
      661 ttccatgatt cagtaatcaa gcgcataccc atcggtagag taggcgaacc aaaggatatt
      721 gccgagatca ttgtgttcct ggcgtcgccg aaagcctcat taattactgg ggcaacgatc
      781 atggcagatg gcggttggac cattcagtga aaagaattgc gcccaacaca gtctccatcg
      841 ctaggcacgt cgcttttgcg acagtcttcg acgtgcagca ttctgaagtg attagaagct
      901 ataaatatat tgaaacaaca cgtggaggaa aaatgtttac aataaaatta gataaaatca
      961 acatcgtcct acttgctatt gcggtcttgc ttggagcatg gtcaattttc atgccagcac
     1021 ccgcaggcgg attttttcca cttgttttgg tgttccttgc catcgcatac atcaggacta
     1081 tttacatgaa ctcaggcaga actgccgcca tagtcatgtc agctatcatt gtgttaattc
     1141 ccctggcttt gtacgcattc tattactttc taacaagctc cagataaaac catcgccaca
     1201 tcgatagtcg atatccaaat aattgcatcc cgaggcacac gaagaaagag gcaccctatg
     1261 ggactcaaac tatttatgct tggcttgact gttcgcgata tgcccagggc gctcgagttt
     1321 tatcgtcgtc ttggactgga cgttcccgag gggagcgaaa cgagcaccca tgtcgaaatc
     1381 ccaatgggga gaggcttcac gttctttctg gactccaatc cagggcggtg ggatcccggc
     1441 tttgacgtaa gcgatcagcc agatctacct gaaagtccgc aatggtatcc gatggttctt
     1501 gaattctatg tcagaaacca aagcgctctg gaagccaaat atgctgaatt aatcgacttt
     1561 ggatatgaga gctgccgtga gccatatcga acatcattcg gtatgtattt tgcaatggtg
     1621 aaggatccgg atggaaacac gatcctgatc tcagcgaaag caaaagataa ttcagaaaca
     1681 gaagtgtaag aatagctgaa acgacctcat agaaattaat ctatctcatg cacatgacac
     1741 tgttgtccag ggactcattt ctagttccta ccccagtaaa attgattctt gcaaccggac
     1801 tattttttct tttcgggtgg gtgatctggc cggtgatttt cactgctttt atgaccgatt
     1861 ggaatcaggt gggctttcct cttgcaatcc gcgcaactgg attatgtgca ccggcgtttg
     1921 tgtgtattga atttcggtgg accgctttgc tcattgatgc cattttctgg tatctggcag
     1981 ctgctgcggt tgtccatgcg cgtgtagggt tttggacgtt ctgcctgtac ttcgttgtta
     2041 ccgttttggg gatgatcctg ctctcatttt tagtgccatt gattgacatt gccttataag
     2101 tagcaatgcg acgcgggctt ccgcatgggg ataaaatatt gatactggtg tgatgacgat
     2161 catcagattc ctgttttagc gatgggaact gatccttctt gaatcaagga gttgccatgg
     2221 acaagcagta tgaagtcatc attattggcg ggcgggttgc ggggtcaagc ctggcgattc
     2281 gactcgcaca acagaacagg aaggtcctgc tgatcgaccg ggcgaccttc ccgagctggc
     2341 ctgcggttcc cagtagtccg ctggttcatc cgggtactat gcggctgctt gatgaggtgg
     2401 ggatgtctga ttcagaatac gcctatccgg agggcaaggt aactcatctc attctggatg
     2461 tgatgggcaa gtatcaggcg atcatgccgc tcgaactcct gcaactggat cggaattacg
     2521 tcttcggtat cgaccgcaac aggttcgaca cgctcctctg gcagaggg
//
LOCUS       DASTJP010000709         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1244098, whole genome shotgun sequence.
ACCESSION   DASTJP010000709 DASTJP010000000
VERSION     DASTJP010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244098"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFQ23_20700"
     CDS             complement(<1..891)
                     /locus_tag="VFQ23_20700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909080.1"
                     /translation="MIPNLPTLKILPLESLILHEDHDNQRTAPLVQRLRRAGILRNPP
                     VVMPLTDGTERYMVLDGANRVTSLTEMEFPHIVAQVVKSDDVHVNLQTWNHVVWGMST
                     KAFIAKIRKIKGLVATKVNTFKSLDAPKYVPGQIRLPDGTLYLLAETPSELKQHIDTL
                     HQIVDAYKTRASLDRTSQTLIDSFKHIYSDLTALVIFPSFKIKTILKLAAQNIILPTG
                     ITRFTVSPRALHLNYPLHELSSGKPMSYKEEYLQEKRNPYYALLPLPFALSDSENQAR
                     EGRGEGEIIHQRETSAPHRIG"
     gene            complement(918..1559)
                     /locus_tag="VFQ23_20705"
     CDS             complement(918..1559)
                     /locus_tag="VFQ23_20705"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HET9909081.1"
                     /translation="MRIIHQDANILVIDKPAGLPVLPDGWEKGAPYLLKILEADHGKL
                     WVVHRLDKTTSGVMVFARDEATHRSLNLQFENHEVEKIYHALLEGNPKWSEKTAKHPL
                     RVNVGHKHRTAVDDKNGKPSETQFKIMKRYEASALVEASPRTGRTHQIRAHAYALGYP
                     LLADTLYSAEETSIIARPALHAQSLTINLNGDQITFQARYPDDFRMALERLKR"
     gene            complement(1556..2155)
                     /locus_tag="VFQ23_20710"
     CDS             complement(1556..2155)
                     /locus_tag="VFQ23_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909082.1"
                     /translation="MELWLARSPWIGVMIWIVLYISDYYLTIYSARGFKEIGHFKFEG
                     SFELTPQYQKDIDALKPISKLHVTLLILYSLVIVAIWYLFVYFLGLPWAYLLYLGMFL
                     LLETAVHLRHFRNLFLIREIKRNGGASGEISYKKWFSYRVSAFELYLYFGLFLMIAAL
                     EYSPFFLGGALICFGNGFKHNRLARKAKSNPTPFVETKA"
     gene            2211..>2545
                     /locus_tag="VFQ23_20715"
     CDS             2211..>2545
                     /locus_tag="VFQ23_20715"
                     /inference="COORDINATES: protein motif:HMM:NF016583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909083.1"
                     /translation="MNEPSANNPFFIDSLLHPLAGPIRKLLTRQGRQELNQILIDDEE
                     NILQALEAGVEIESLYFAGEEIISENLRKNLTEGATIHEVAKRTTKKLFENDKISRIF
                     AIAETPKSVG"
BASE COUNT          661 a          612 c          604 g          668 t
ORIGIN      
        1 tccgattcta tgcggagcag acgtttctct ttgatgaatg atttctcctt caccccgacc
       61 ctctctcgct tggttttcgc tatcgctcaa tgcgaatggg agaggaagta aagcgtaata
      121 tggatttctt ttttcctgaa gatattcctc cttataggac atcggtttgc cgcttgataa
      181 ttcatgaagt ggatagttca gatgtaatgc acgcggcgaa acggtaaaac gcgtaattcc
      241 ggttggtaga atgatattct gcgccgccag tttgagaatt gttttgatct tgaaacttgg
      301 aaatatcacc agagccgtca aatctgagta aatgtgtttg aacgaatcga tcaacgtctg
      361 gctggtacga tcaagtgatg cccgcgtttt gtaggcatcc acaatctggt gtaaagtatc
      421 tatatgttgt ttgagctcac tgggcgtctc agccagcaga tacaaggtgc cgtccggcag
      481 cctgatctgc cccggtacat attttggtgc atccagcgac ttaaacgtat tgacttttgt
      541 ggcgacaagc cctttaatct tgcgaatttt ggcgataaac gcttttgtac tcattcccca
      601 aaccacatgg ttccaggtct gcaaattgac atgcacatca tcagacttaa ccacctgcgc
      661 aacgatgtga ggaaattcca tttccgttaa agatgtgacc cggtttgctc catccagcac
      721 catgtagcgc tccgttccat cggtcaaagg catcacgacg ggtgggttgc gcagaatgcc
      781 cgctctacgc agtcgctgta caaggggcgc agttcgttga ttgtcatgat cttcatgcag
      841 gattaatgat tcaaggggca atatcttcaa agtgggcagg ttgggaatca tgatttcctg
      901 agcataccat acaaggacta gcgcttcaaa cgctccagcg ccatccgaaa gtcatcaggg
      961 tagcgtgcct gaaatgttat ttgatcgcca ttcaagttga tcgtcaatga ttgagcgtga
     1021 agagccgggc gtgcgatgat acttgtttct tctgcgctat acagcgtgtc cgcaagaagc
     1081 ggatatccca aagcgtaggc atgggcgcgt atctgatggg ttcttcccgt ccttggactc
     1141 gcttcaacca acgccgaggc ctcataccgt ttcatgatct tgaactgcgt ctctgaaggt
     1201 ttcccgtttt tgtcatcgac cgctgtgcga tgtttgtgtc ctacatttac tcgcaatgga
     1261 tgttttgcgg ttttctcact ccattttgga tttccttcaa ggagggcatg atagatcttt
     1321 tctacttcgt ggttttcaaa ctgaaggttt aatgagcgat gcgtagcttc atcccgcgcg
     1381 aagaccatca caccgctggt ggtcttatcc aaacgatgga caacccaaag cttgccatga
     1441 tctgcctcca atatttttag gaggtagggc gcgccctttt cccagccatc cggaaggacc
     1501 ggaagcccgg caggcttgtc tattaccaga atgtttgcgt cctggtgaat gatcctcatg
     1561 cttttgtttc aacaaatgga gtggggtttg attttgcttt ccttgccagg cgattgtgct
     1621 tgaaaccatt tccgaagcat attagtgccc caccgagaaa gaatggactg tattcaagcg
     1681 cggcgatcat caaaaagaga ccaaagtaca aatacaattc aaaggcagac acccgatagg
     1741 aaaaccattt tttgtaggaa atctcccccg atgccccgcc atttcttttg atctcgcgaa
     1801 tcagaaagag gttacggaag tgtcgcaggt gcacagcggt ctcaagcagg aggaacatgc
     1861 ccagataaag caaatacgcc cagggtagcc cgagaaaata aacgaacaaa taccaaattg
     1921 ccacgattac aaggctatac agaataagca gggtaacgtg tagcttagaa ataggcttga
     1981 gggcatcgat atctttctgg tactgaggag taagctcgaa actgccttcg aatttgaaat
     2041 gcccgatttc cttaaaccct cgcgcggaat aaatggtcaa ataataatca ctaatataca
     2101 ggacgatcca gatcatcacc ccgatccagg gactccttgc cagccataat tccatgcgga
     2161 aattataacc ctgggaagaa gctccacatt ggcagggtta taatatttat atgaatgagc
     2221 cctcggcaaa taacccgttt ttcattgatt cgcttctcca tccactggcg ggacccatcc
     2281 gcaaattgtt gacgcgacag ggccggcagg aactcaacca aattctcatc gacgatgagg
     2341 agaacatcct tcaggcgttg gaagcgggcg tggaaattga atccttgtat tttgctggcg
     2401 aggaaataat ctctgagaac ctgcgcaaga acctaacgga gggcgcgacc atccatgagg
     2461 tagcgaaacg caccacgaaa aaacttttcg agaacgacaa gatctctcgc atcttcgcca
     2521 tcgccgaaac gccaaaatcc gtggg
//
LOCUS       DASTJP010000710         3360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1012473, whole genome shotgun sequence.
ACCESSION   DASTJP010000710 DASTJP010000000
VERSION     DASTJP010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012473"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFQ23_20720"
     CDS             complement(<1..345)
                     /locus_tag="VFQ23_20720"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9909084.1"
                     /translation="MQLKEYLEQLYDYNYWANKRYLAVAETLTEEQLFLKQGHSWDSV
                     HAVLVHMMSSERMWPQRWRGETGTFLDAKDFPTMTSIRDYWVDVEKAMRSFLAEQSEG
                     SLLQDVSYTNPRG"
     gene            complement(355..1083)
                     /locus_tag="VFQ23_20725"
     CDS             complement(355..1083)
                     /locus_tag="VFQ23_20725"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET9909085.1"
                     /translation="MKRRGAGAFAEQLKFQHKATRKIKPDEPVQTWLQLPRGNKAVLP
                     ARFQHEDVRYADSLVEYCIKNFTEDGQVVFDPFAGFGTTLLIAEEMGRVGYGIEYSKQ
                     KADYIHSLLEHPERLIHGDSRNLLEYDLPLFDLCLTSPPYTNQSDDENPFVDYRKKGF
                     DYPSYLGEMKHIFSQVSQKLNPSGHVVVEASNLKKNGQVTTLAWDITRELAQIFHFHG
                     ETVICWDKYGYGYNHSYCLVFSKA"
     gene            complement(1104..1568)
                     /locus_tag="VFQ23_20730"
     CDS             complement(1104..1568)
                     /locus_tag="VFQ23_20730"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9909086.1"
                     /translation="MAKKQTFTATILNAGGGGAYVEVPFDVEAIFGSKKTKVKATLEG
                     VPYRGILTRMGTEHHVLIILKGIREKINKTFGDEVNITVELDTEPRVIELPSELTKAF
                     KTETDAKAFFDKLSYTHQREYVMWINEAKREETRQNRIAKTIEMLKQGKKAR"
     gene            complement(1572..2267)
                     /locus_tag="VFQ23_20735"
     CDS             complement(1572..2267)
                     /locus_tag="VFQ23_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909087.1"
                     /translation="MIKMDNRDKINEYDLVEIIQVPERLEGIVDIGDIGVVLEKYDET
                     SFKVECIQPGGSYKWLRMLDARHIRLRSRDPFSNWAKKSLREKSITETSITAGVVVGA
                     ASGALIGVGLGAITKSLNGLLTGLIIGLILGAVTGALTAALTVRTAGTTGGIGVGYFT
                     GMIFGGVFGMTLGALVPASFRMRAQTDDLPLLDALMIGSFETAMLAGFLLSILATVVG
                     VWVAGKNLVEEKA"
     gene            complement(2390..2980)
                     /locus_tag="VFQ23_20740"
     CDS             complement(2390..2980)
                     /locus_tag="VFQ23_20740"
                     /inference="COORDINATES: protein motif:HMM:NF022167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909088.1"
                     /translation="MLNFDPVFQATQIFTNYDRPWGVCGGWGIDLFLNRVTRSHKDID
                     FAILRRDQLIIQEYLLSRGWALEKAVSGELIPWRRGERIDLPVHTIWCKNPKAVPDFI
                     ELLFNEVDSTDFRFRRDRSITLPFEKMILLSALDIPLLAPEIVLLYKSVKPDDPSAAA
                     DFRNALPELSSASRAWLVVALNKLYADHIWLQDLAR"
     gene            complement(2981..>3360)
                     /locus_tag="VFQ23_20745"
     CDS             complement(2981..>3360)
                     /locus_tag="VFQ23_20745"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET9909089.1"
                     /translation="EMLFRALSGMNKDEILFDATVKISSPKIIDISNGNYRQKSVNQI
                     RGSGYVVESLEAALWCFYQTNTFEAAILQAANLGDDADTTAAICGQIAGAYYGESGIP
                     LKWFERLHMQAEMTELADKLSIE"
BASE COUNT          832 a          879 c          737 g          912 t
ORIGIN      
        1 gcctctggga ttggtatagg aaacgtcctg caggagtgat ccctccgact gctcggcgag
       61 gaagctgcgc atggccttct cgacatccac ccaatagtca cgaattgatg tcatcgttgg
      121 aaaatctttg gcatcgagaa atgttccggt ttctcctcgc cagcgttggg gccacatccg
      181 ctctgaactc atcatgtgaa ccagcacagc atgaacgcta tcccaactgt gcccctgttt
      241 gagaaaaagt tgttcttccg tcagcgtttc agcgaccgcc agatagcgct tgttcgccca
      301 gtagttatag tcgtaaagct gctcaagata ctctttgagt tgcatacatg ctcctcaagc
      361 ttttgaaaat accaaacaat agctgtggtt atagccgtag ccgtatttgt cccagcaaat
      421 gacagtctcg ccgtggaagt gaaagatttg agcaagctcg cgcgtaatgt cccaggctaa
      481 cgttgtgact tgaccgttct ttttcaggtt ggatgcttcc accacaacat gacccgatgg
      541 attcagcttt tgggagacct gtgaaaagat gtgtttcatt tcccccaaat aagaggggta
      601 gtcaaatcct ttttttcgat agtctacaaa cggattctcg tcatcactct gattcgtgta
      661 gggtggtgag gtaagacaaa gatcgaagag cggcagatca tactccagca ggtttcgaga
      721 gtcaccgtgg atcaaacgct cgggatgttc gagcaggcta tgaatatagt ctgctttttg
      781 tttgctatat tcaattccat atccgaccct gcccatttct tcggcaatta acagagtagt
      841 gccaaacccg gcaaacggat caaagacaac ctgcccatct tccgtaaaat tctttatgca
      901 atactcaacc aggctatccg cgtaacggac atcttcatgt tggaaccggg ctggcaggac
      961 tgctttgttg cctcggggta actgcagcca ggtctgcacg ggttcatcag gtttgatttt
     1021 ccgagttgcc ttgtgctgaa acttcaattg ttcagcgaat gcacccgctc cgcgtcgttt
     1081 cacgccttat ctccttggtt gatttatctg gccttcttgc cttgtttcaa catctcaatc
     1141 gttttcgcaa ttcgattctg gcgcgtttct tctcgcttgg cttcattgat ccacatcaca
     1201 tattcccttt gatgtgtgta cgagagttta tcgaaaaaag ccttcgcatc cgtctccgtc
     1261 ttgaacgcct tcgttaactc agacgggagt tcaatcacac gcggctctgt gtccaactca
     1321 accgtgatat tcacttcgtc cccaaaagtt ttgttgatct tctcgcgtat ccctttcagg
     1381 ataatgagca catgatgttc tgttcccatc cgcgtcaaga tgccgcgata gggcactcct
     1441 tcaagcgtgg ctttgacttt ggtttttttt gagccaaata tggcttctac atcgaaaggc
     1501 acttcaacat acgcaccacc cccgcccgca tttaggatgg tggcagtaaa tgtttgtttt
     1561 ttcgccatgc gctacgcctt ttcttccact agattttttc cggcaaccca gacgccaaca
     1621 accgtagcca aaatggatag cagaaaaccg gcaagcatcg ctgtttcaaa gctccctatc
     1681 atgagcgcgt caagcagagg taaatcgtca gtttgggctc tcattcgaaa cgaggcagga
     1741 accagggctc caagggtcat tccaaacacc ccgccgaaga tcatgcccgt aaaatatccg
     1801 acgccaattc cgcccgtcgt cccggccgtc cggacggtca acgcggcagt gagagcgccc
     1861 gtcaccgctc caaggatcag accgatgatc aatccggtca acagaccatt gaggctcttt
     1921 gtaatcgcgc cgagtcccac tccaatcaat gcgccagagg ccgcgcccac taccacacca
     1981 gctgtaatgc tggtctctgt gattgatttc tctctcagag acttcttcgc ccagttgctg
     2041 aacggatccc tgcttctcaa ccggatatgt ctggcatcca acatcctcaa ccatttgtaa
     2101 gaaccacccg gttggataca ttcgacttta aagcttgtct catcgtattt ttccaggaca
     2161 accccaatat cgccgatgtc tacgatcccc tccaatcgtt ctggcacctg gataatttcg
     2221 accaaatcgt attcattaat tttgtcacgg ttatccatct tgatcatggt ttatcatcct
     2281 gagaaataac aagaagccgg ttactacatc cattttaccc tttgtctccc cgccggccgc
     2341 gctcgtgatt cggatagcaa ttttagatag tctttgtcat tgtactccac tacctggcca
     2401 agtcctgaag ccagatatga tctgcataca atttattcag agcaacgaca agccaggcgc
     2461 gcgatgcaga ggaaagctcg ggcaaagcat tcctgaaatc tgcggcagcg gatgggtcat
     2521 ccggtttgac agatttgtac aataatacaa tctccggggc aaggagtggg atgtccaatg
     2581 ccgaaagcag tatcattttc tcaaagggca gtgtgatcga cctatctcga cgaaaccgga
     2641 agtccgttga gtccacttcg ttaaatagta gctcaataaa atcaggtaca gctttgggat
     2701 ttttacacca gatcgtatga acagggagat cgatccgctc gcccctccgc cagggaatca
     2761 gttcaccact gactgctttt tcgagagccc agccgcgaga taaaagatat tcctggatga
     2821 tcagctgatc tcttcggagg atagcgaaat cgatatcttt gtgtgaacgt gtaacacgat
     2881 ttaaaaacag gtcgattccc cagccgccac aaactcccca gggacgatcg taatttgtga
     2941 atatctgtgt ggcttgaaaa acagggtcaa agttcagcat tcattctata gaaagcttat
     3001 cagctagttc ggtcatctca gcttgcatat gtaaacgttc gaaccatttc aaggggattc
     3061 ctgattcacc ataatatgca ccagcaattt gaccgcagat tgctgcggtg gtatcagcgt
     3121 catccccaag atttgcagct tgaaggatag cagcttcaaa tgtattagtc tgataaaaac
     3181 accagagtgc agcttccaaa ctttccacca catagcctga tccacgaatc tggttaactg
     3241 acttctggcg ataatttcca ttggaaatgt cgattatttt tggcgatgag attttgacag
     3301 tcgcatcaaa taggatttca tccttgttca tcccagataa cgcgcgaaaa agcatttctg
//
LOCUS       DASTJP010000711         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1202673, whole genome shotgun sequence.
ACCESSION   DASTJP010000711 DASTJP010000000
VERSION     DASTJP010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202673"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="VFQ23_20750"
     CDS             complement(<1..1669)
                     /locus_tag="VFQ23_20750"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9909090.1"
                     /translation="MKATSRLDRIVSLIDEHSFLTVSDLSRLCDVSEMTVRRDLDQLQ
                     KQNRILRTFGGAVPVRTESLPDNGAAHPHDQRAEVLLVDQVDVLIGTSVNPYYDNLLI
                     DRAIKKDIAIIAESIEMPNQRTVVAVDNYQAAYDLGTWTGKYLKQQGVKKVNLLDLTF
                     HQPNTQNRSRGFADGLGKINSSFETILSINAQSRYATAYQLSKDALIVHPHINLIFAI
                     NDTTAWGAINACRDLNIDPAQMTIVTFGLEGETLKNELMSQNSYCKAGLAMFPEIVSL
                     TCIEAAIAAFNHQPQADKYITPHVVLTAATLPKYYKRTAAGWELDWETARKNLNIPIK
                     IEREKPEPTTKLPRQIGLIVPFLEHEWYKNLTALLKKYSEQYGIGLQIIDADQNVRDE
                     VDLRRRQIAIKAATLVNPGDVVLIDGGPIAPYLARELKQKKDITVITNSVFVFDTLNR
                     TPDIILISTGGAVRYSTQMLVGPTAEGALKELRADKLFLMVSGISLDFGLSHHTISEV
                     TMKQAMIRSAREVILLADHTAFGAEVGIQVAPLKAVHWLITDDVLPPS"
     gene            1999..>2468
                     /locus_tag="VFQ23_20755"
     CDS             1999..>2468
                     /locus_tag="VFQ23_20755"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9909091.1"
                     /translation="MYNKRIFSVVAIIAMLAMLMTACGAAQAPAEGEKTLTIAGVVFQ
                     DDQFMKSMVQGYEDAGVKYGIKVMTANTNNDQAKEAELIQTYIAQGVNGIAIAPLSQD
                     ASIPNLKEATSKGIQVAITNMNLAGDSSWLAGGYTSDDATNGKIVGTNAAEFIKA"
BASE COUNT          621 a          574 c          603 g          670 t
ORIGIN      
        1 tactaggcgg taacacatca tctgtgatta accagtgaac tgccttcagc ggtgcaactt
       61 gaattcccac ttcagctcca aacgccgtat gatctgccag taatatcact tcgcgtgctg
      121 aacggatcat ggcctgtttc atggtgactt cagagatcgt atgatgggat aatccaaagt
      181 caagtgaaat tccgctgacc atgaggaaca acttgtccgc ccgcagttcc tttaaggcgc
      241 cttcggctgt tggtccgacc agcatctggg tactataccg cactgctccg cctgtggaaa
      301 tcaaaatgat atccggcgtt cggttcagcg tatcgaacac gaacacagaa ttggtgatca
      361 cggtgatatc ctttttttgt ttcagctccc tcgcaaggta gggagcgatg ggtccaccgt
      421 cgattaatac aacatcacct gggttcacaa gagtcgcggc tttaatcgca atttgccggc
      481 ggcgcaggtc cacttcatca cgcacatttt ggtcagcatc gatgatctgc aatccgatcc
      541 catattgttc cgaatatttt tttagtaggg cggtcaggtt tttataccac tcgtgctcaa
      601 ggaaaggcac gatcaagcca atttgtcgcg gcagctttgt cgtgggctcg ggtttttcgc
      661 gctcgatctt gatcggaatg ttcaaattct ttcgagcggt ttcccagtcc aattcccaac
      721 ccgctgcagt tcgtttgtaa tatttgggta gcgttgcggc tgtcaggaca acgtgagggg
      781 tgatgtattt atctgcttgt ggctggtggt tgaatgcagc aattgccgcc tcaatgcagg
      841 ttaagctcac aatctcgggg aacatggcca ggccagcctt gcaatagctg ttctgactca
      901 tcaactcatt tttcagtgtt tccccttcca gaccaaatgt aacaatggtc atctgtgccg
      961 ggtcgatatt taaatcgcgg caggcattga ttgctcccca ggccgtcgtg tcattgatgg
     1021 caaagatcaa gttgatatgt ggatgcacaa ttaatgcatc ctttgagagc tggtacgcag
     1081 tggcgtatcg ggattgggcg ttgatggaca ggatcgtttc gaatgatgaa ttgatcttgc
     1141 ccagtccatc ggcgaaacca cgactgcggt tttgcgtgtt gggttgatgg aacgttaggt
     1201 ccagcagatt aactttttta accccttgtt gcttaagata tttgcctgtc caggttccca
     1261 ggtcataggc tgcctggtag ttgtcgactg ccacgacagt gcgttgattg ggcatctcta
     1321 tcgattcggc aatgatggct atatcttttt ttatggccct gtcgatcagc aggttatcgt
     1381 aataggggtt gacggaagtg cctatcaaca catccacctg atccactaat aagacctcgg
     1441 ctctttgatc gtgtggatgt gctgcaccat tgtcgggaag agattcggtc cggacgggca
     1501 cggccccgcc aaaggttcgg agaatgcgat tctgtttttg aagctgatcc aggtcccggc
     1561 gaacggtcat ttctgacaca tcgcaaagac ggctgagatc gctcaccgta aggaagctat
     1621 gctcatcaat gagtgaaacg atcctatcaa ggcgggaagt ggctttcatt tagcgtcctt
     1681 tttcaaagat tactgaaaca taaaggcatt atactcgaag aatgttaaat gtcaaacaaa
     1741 attgaataaa actcgaacat tatttgcttg actttaaatt cctattgtgc taatatgcac
     1801 aagtgggcgg tttattttat agacattaac aaaaaaatga gtattttggt agtttataaa
     1861 catttatcgg tgggaatcgt tcgaataaca ttaattcact acgacctcac agagaaggag
     1921 ctgacaagat atcaaatacc ctattccctt ttcgaaactc aaaacgttcc attttgtaac
     1981 caatctaagg agaagaaaat gtacaacaaa cggatttttt cagtcgtagc gatcatcgct
     2041 atgctggcaa tgttgatgac agcctgtggc gcagcgcagg ctccggcgga aggtgaaaag
     2101 acactcacca ttgcaggcgt tgttttccag gatgaccagt tcatgaaaag catggtccag
     2161 ggctatgagg atgctggtgt caagtatggg atcaaagtca tgaccgccaa caccaacaat
     2221 gaccaggcga aagaagccga gctcatccag acctatattg cccagggcgt aaatggtatt
     2281 gcgattgccc ctctgagcca ggatgcttcc atccccaact tgaaggaagc aacatccaag
     2341 ggtatccagg tcgccatcac caatatgaat ctggctggtg attcttcctg gctggccggt
     2401 ggctatacct cggacgatgc caccaacggc aaaatcgtgg gtactaatgc cgctgaattc
     2461 atcaaagc
//
LOCUS       DASTJP010000712        17102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_924127, whole genome shotgun sequence.
ACCESSION   DASTJP010000712 DASTJP010000000
VERSION     DASTJP010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17102
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924127"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFQ23_20760"
     CDS             complement(<1..325)
                     /locus_tag="VFQ23_20760"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9909092.1"
                     /translation="MHQESEVLTTIIAAVGFGLFAQVLAHRWSIPAIVLLLLSGILLG
                     PHGFHLIEPEALGEGLEILVKLAVAVILFEGALNLRLQTLRHSALEVRNLVTIGVLIS
                     WAVTSF"
     gene            677..1876
                     /locus_tag="VFQ23_20765"
     CDS             677..1876
                     /locus_tag="VFQ23_20765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909093.1"
                     /translation="MKRKKTLPPHSKKKEMIGIQVFDQILRTAPASLRRPILAAAHQL
                     LGALQQSEVRFQSVFENTVRGMALLNSTGEIMESNLALHNILGYSGEELKGKTLIPYI
                     HPGDAELDPTLNQALSAHEAGDYEDEKRFVRKDGQVRWGRWIVSHVYLEHGQNPAFAL
                     AMLEDITERKKDQQFLVQSESLSIVGRLGASLAHEINNPLQAALGCLGLAEEALEEGA
                     DVRRYLAIAMEELERAAGIVSRLRNLTRTPKEKKKQPVDLNALIEQSLVLIRKRCQDQ
                     AIEVKWRRKSDLSPVSIVADNNRQVFLNLMLNAVEAMPDGGQLKISTISTGRPRGVRV
                     TISDTGKGIEPDSLSNIFEPFYTTRPDGMGLGLYISKKIVEDQGGRLEVESRIGKGSK
                     FSVWLPG"
     gene            1887..2603
                     /locus_tag="VFQ23_20770"
     CDS             1887..2603
                     /locus_tag="VFQ23_20770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909094.1"
                     /translation="MTKSLRALVVDDEEGVRFFLIETLQRMGHVVTEASSGEEALDHL
                     RDTAFDLAILDLKLGGPADGQRVLESIRWRWPATMVIMLTAHGSLESAVEAIHEGVDG
                     YLLKPVRPIDVRRAVQKALHRRQKLIVAEPEPLDHRLQRGPFHIDLERYEAKFEGQIL
                     SLSPRDFALLHYLMQKAPQVVSAAELVKVIRQYEPEYAGEARTMIKWYVHQLRKKVEP
                     NPAKPRYILNVRGVGYRFVG"
     gene            2852..3277
                     /locus_tag="VFQ23_20775"
     CDS             2852..3277
                     /locus_tag="VFQ23_20775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909095.1"
                     /translation="MQGPYRILVVDDDPKVLFMLRESLEKLGNGYGIATAADGQQALV
                     KAQNQPFDLIISDVKMPGIDGIQLVEMIRSLNIETMLIWITALGCESLKAECERLSVS
                     SCLQKPLRISEIRDAVQAALSSKDENRQGHGNERRSEND"
     gene            3270..3974
                     /locus_tag="VFQ23_20780"
     CDS             3270..3974
                     /locus_tag="VFQ23_20780"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9909096.1"
                     /translation="MIRMELKEGTSVFTTGGKEVGKINRFVLDPTTNEVTHIVVQKGW
                     LFSEDKVVPFGMVNSATMERVVLSENIDTFDQLPPFEERHYVKAGESEGSDSHDQQSD
                     QDEIRKVDVRREGRLRDDDFPSYYWYPPHGYVGYPIGVYGWPSLETTRNIPGNTISLK
                     EGTDVVSSDGKHVGDIERLFVASDSNKVTHFVISQGLLFKDRKLIPAHWVRTADEGKV
                     DLAVSTQLLKDLPTYE"
     gene            4160..4315
                     /locus_tag="VFQ23_20785"
     CDS             4160..4315
                     /locus_tag="VFQ23_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909097.1"
                     /translation="MNALNIIADHSLDGKIYRVTSALLASELLLDWQGKFFVVSLIFS
                     QIDKHWD"
     gene            4367..5983
                     /locus_tag="VFQ23_20790"
     CDS             4367..5983
                     /locus_tag="VFQ23_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET9909098.1"
                     /translation="MKTVIAAALGECVHVAGVSNFLRLAEMAGWRTVFLGPAVSVERV
                     LEAARAEKAELIGVSYRLTPETGERLLGDFAEEAADLHEAGVKFAFGGTPPIAERASK
                     LGFFEKIFDGSESPELVLEYLKGQSTSDMVESGYPQSVTQRIAWRSPYPILRHHFGLP
                     SMEETIDGIKKIAEAGALDLISLGTDQDAQENFFHPERQDPRRKGAGGVPVRSAEDYR
                     MLFEASRVGNFPMLRAYSGTDDFIRYAEVLTETIHNAWCAIPLFWFNQMDGRGPWDLE
                     GSIREHQKVMAWYGERNIPVELNEPHHWGMRDASDVIYVVSAFLAAYNAKKFGVSDYI
                     AQMMFNSPPGTADAMDLAKMLAVLELINPLADEDFRIWKQTRIGLLSHPLDSDAARGH
                     LAASTYLQMALKPHIYHIVGHTEAHHAATADDVIAASRIVRRAITNALGAPDMTQAKE
                     VQQRKKKLISEVRFTLGVIRDLADIGVDDPWADPATLTKAVRTGVLDAPQLLNNKFGC
                     GVIRTRIIDGACEAVDPSGHIISEKERLSK"
     gene            6019..7119
                     /locus_tag="VFQ23_20795"
     CDS             6019..7119
                     /locus_tag="VFQ23_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HET9909099.1"
                     /translation="MTEKYTVIGAGHGGKAMAAHLALMGLDVALWNRNFDHISIIKQR
                     GGIELESFDGGPHGFGNLSLVTPDLEQAVAHAQIIMVVLPSSAHAGMAKAVAPYLRDG
                     QIVVLHPGRTLGALEFTKVIRDNGCTADVTVAEAETFIYASRAEGPAQARIFRIKEAV
                     PLAALPACRTEMVLNAIQCAYPQFIDGEDVLHTGLNNMGAIFHPALTLLNAGWIEHTH
                     GDYQFYIDGVTPSVARVLEVLDRERVTVASSVGIRARTSLEWLKLAYNTAGENLHEAI
                     HNQPGYHGIKAPATLNHRYIFEDVPMSLVPIASLGKRYGVSVQGMESVIRLGSIIHRT
                     DYWRRGRTLERLELERWSVSELTRFVREGIVE"
     gene            7219..8712
                     /gene="asnB"
                     /locus_tag="VFQ23_20800"
     CDS             7219..8712
                     /gene="asnB"
                     /locus_tag="VFQ23_20800"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF006949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HET9909100.1"
                     /translation="MCGIAGVFQRPANQIVKSMVRNIAHRGPDGLGVMDVPYGTFGHT
                     RLAILDLSEGRQPMEYRDTTIVFNGEIYNYQDLQRKHLTNYNVRTRSDTEILLHLYRK
                     FGPECVELLDGMFAFAIQHNNDLFLVRDPIGIKPLYYGTSHDGKHFIFGSEIKALDGV
                     VNSITEFPAGHWFHSRLGWHKYFHLEEKIRLFQGTEHDVQPVIKSTLRDAVHKRLLAD
                     VPVGVSLSGGLDSSIVTSLAREESEQLHSFAVGVMGSPDLEAARQMGKHLDTIHHEYI
                     YTVPDMIAALPHVLYYLESFDPALVRSAIPNYFLAKLASDHVKVILTGEGADEIYAGY
                     DYLAGFQSPDALQGEMLSITNALHNTNLQRADRMSMAFGLEARVPFLDVQSISLALGI
                     PPSWKIHAGRAPKSLLRQSFADDLPKEIVNRPKAKFSQGAGSSELLAQQAVEKISDQQ
                     FMSERKRLKEDWNYNLQNKEALFYYRILHEFYDDKWILPTMGNSRSL"
     gene            8718..9293
                     /locus_tag="VFQ23_20805"
     CDS             8718..9293
                     /locus_tag="VFQ23_20805"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HET9909101.1"
                     /translation="MLETFDWYSWVILPLIVFIARVLDVTLGTMRIIFLSRGRKYLAP
                     ILGFVEVLIWITVVSQIVGSAQNPVAYIAYAAGFATGNYVGLSIEDKLAIGTLVIRII
                     VTHNGKSLINNLQGAGYGVTSVDGQGATGPVILVYTVVVRKELEQVIEIIEGSSPKSF
                     FTVEELRSVRQGIFPQRRRLPDEILLRRKSK"
     gene            9480..11051
                     /locus_tag="VFQ23_20810"
     CDS             9480..11051
                     /locus_tag="VFQ23_20810"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9909102.1"
                     /translation="MYDLTAFNLKNMTTLGVALRQMGMGASSMEEVANKIVRYLYENL
                     GDGTGKPISALVEFFITYPYQKLETELQQHVRHVLGMRPADPDLSCQTLLATVGEEPN
                     WNDRRRSVYYKVLPLTEGIVDDTPMFAQVSEVFDIVMKSRTKPNPESLVELEQKTYNV
                     FYVPDAAGSDHVPEQEDFVIRYGLRSILGFYALLPSGNLFTVIVYSKTRIPSATTEFF
                     KTLALNVKISILPFDDVAVFAGSTPDGLNTIPQNELIHFRSQAASLAQLLKVRDRIVV
                     QQSDRIEQVMVELREQARELERTNYELESTIAENVRLRQLAAETAVIEERNRLSRDLH
                     DSVTQALYGQTLYAEAAARQLDAGMVAQAAEHLRQLRHTAQQALREMRLLIFELRPSA
                     LETEGFAAALQARLESVEIRTGLEANVNIDEELHLTPEMENDLYWIVQEALNNTLKSA
                     EAKKVVVSLSRESNQMILQIADDGKGFDPEISSNARGLGLRSMEERSEKLGGHFHIES
                     QPGTGTVVRVEVPFG"
     gene            11044..11700
                     /locus_tag="VFQ23_20815"
     CDS             11044..11700
                     /locus_tag="VFQ23_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909103.1"
                     /translation="MGEPIRIFIADDHAIVRSGLRALLATEPEIEVVGDAANGEQAVT
                     GVKERQPDVVLMDLEMPGMDGIEAIRQIIGADPEARILVLTGFATDERVFPAIQAGAL
                     GYILKDAGPEALVQAIRQVYRGESSLHPKIARKLIEEMARPVDQPPTADPLTPREVEV
                     LQLVAKGMSNQEIAGKLVIGEATVRSHVNNILRKLHLASRTQAALYALREGYASLGDS
                     "
     gene            11877..12290
                     /locus_tag="VFQ23_20820"
     CDS             11877..12290
                     /locus_tag="VFQ23_20820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909104.1"
                     /translation="MYEQNQPEERKSLRLLIVDDNASARSGMKALLETVASHKWKPDI
                     FEAASGPEAIDLVETLQPDLILMDAHMPTFNGIEATRIIKQRWPTIKVIILTLYDAYR
                     DIALAAGADLFLLKGGSSEELFSAIYNVSIGQDKS"
     gene            12313..13563
                     /locus_tag="VFQ23_20825"
     CDS             12313..13563
                     /locus_tag="VFQ23_20825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9909105.1"
                     /translation="MSRRKKSVNTAVIVAVLLVLFMGARAKQDDELTYAGSQLEGATY
                     SERGPYTVGVRDLIIQGKRPLEISLWYPAANGEGGEEVTQYPYEIKMAKPIGKFAVAT
                     FAGRALRDVSPDLSDVPYPLVILSPGFSLGSFAYAWLAEHLASYGFVVIAPEHQEHLD
                     PANELWRSAIERPQDVLSVLAYVDEQTADGGVFDGLINAERIAVIGHSYGGYTSLASA
                     GARINTPAFKSHCEEASASNHPAAWLCNEILPHLDDMADLAGLDETPNGLWPAWTDSR
                     IVAIVPMAGDAFFFGQEGLAEINIPVMAIGGTKDNDAPYEWGTLPAFEHASSPRKVLI
                     SLTDAEHMIFTNPCEKIPFYLKLFSREFCHDTDWDRKYAHELVSHFTAAFLLAELKQD
                     LAASQALAPNNIELPGMKYEAQGY"
     gene            13842..14600
                     /locus_tag="VFQ23_20830"
     CDS             13842..14600
                     /locus_tag="VFQ23_20830"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9909106.1"
                     /translation="MSSNSLALEKNKMTNTAGATSQLNVGRIAGLLYLTIIVSGIFAE
                     FFVRQSLRVPGDAAATASNIMASASLFRLGIAADLVMIMSDVALALIFYVLLKKVNQS
                     LSLLAAFFRLGQATILGLNLLNLFAVKQLLSGESFLSTLEAGQLNSLAYLFFNAHGTG
                     YSIAMVFFGFSILVLGYLIFKSGYIPKVLGAMLMLASVGYLVDSFAKFLMPNYASYEG
                     VFTMVVFVPAFISELALCLWLLIKGLKVQPQEIN"
     gene            14614..15324
                     /locus_tag="VFQ23_20835"
     CDS             14614..15324
                     /locus_tag="VFQ23_20835"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9909107.1"
                     /translation="MKSIQKTARFAGVLYLIITVAAIVAHQYVPSMLIVPGDAATTAS
                     NIAASETLFRIGGVGSELIILLSEIVLAVVLYVLLKPVNKTLSLIAAVSRLAMTTIHG
                     LNLLNYYFVLSLVGDTGYVAAFAPEQVNALVSLFLDAHHYGFTIGIAFLTIHVFVLGY
                     LILKSGYFPSVLGILFLAAGLGYLVDSFALLLSTSYDTTPIFLAIPIALAEIAFPFWL
                     LTKGINVEQWRKSVLAPA"
     gene            15450..16001
                     /locus_tag="VFQ23_20840"
     CDS             15450..16001
                     /locus_tag="VFQ23_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909108.1"
                     /translation="MTRSLEEQREEFARRRFIAMPIAGIIAWSIAGISGAFLPKIYAV
                     WVLFISTGSIVYLGILISKFTGENFLDKKKDKNEFDTLFIYTVGMAVLVYSIAIPFFL
                     VDYESLPLTVGILAGLMWVPFSWIVGHWVGIFHTLLRLVLVVAFWYLLPDSRFVAIPV
                     VIIAVYFATIVILENRWRRLETG"
     gene            16042..16959
                     /locus_tag="VFQ23_20845"
     CDS             16042..16959
                     /locus_tag="VFQ23_20845"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9909109.1"
                     /translation="MKAIICTKSGPPEVLQLREVERPVPRDNEVLIRVHASTVTQGDV
                     MLRKLHPLLFLPMRLFGVKQKRTPGHEFAGVVDGVGKAVKRFRKGEAVFGTTTGLSVG
                     ANAEYVCLPEEWSDGVLALKPSNATFEEAAALPVGGMTALYILRQANIRRGQKVLVYG
                     ASGSVGTYAVQIARQYFGAKVTGVCSTRNIDLVRALGAEMVIDYTREDITSSGETYDV
                     IFDAVGKLSSSKTRNMLKENVTFLTVQSSTTEELENLIVLRNLMEEEKLKPVIDKCYS
                     LEQAAEAHRYVEAGHKKGNVVISVGYSVN"
BASE COUNT         4380 a         3927 c         4564 g         4231 t
ORIGIN      
        1 tgaagctggt cactgcccac gaaatgagaa caccaattgt cacaagattc cgaacttcca
       61 atgcgctatg ccggagggtt tggaggcgga gattcagcgc cccttcgaat agaatgacag
      121 caactgccag cttgaccagg atttcgagtc cttcccctag agcttccggc tcaatcaaat
      181 gaaatccatg gggtccaaga aggatgccgg ataagagcaa caaaacaata gctggtatgc
      241 tccagcgatg cgccaggact tgagcaaaca gaccaaagcc cacagccgcg attatcgttg
      301 tcagtacttc ggactcttga tgcataaatt gtgtcggaaa ctgatgttgt ttgaacgatt
      361 atacccaaga aaaagaaatg ccaatcagct cctggtcatg gggattggct aatttcgtgg
      421 gcttagccac tgagatcaca gagacctgag agaattttct caaagaactc agtgttctcc
      481 cccggcactg cccgccaggg caagtgtgtg gtgaatccta ctctagccca tccttttatc
      541 cactaccctt ttccctaaag tgcaaaaaat gtcatgatat actggttctg gggaaaattg
      601 gacgtctctt tgcagattca aggacgtgtc ttcagtatca ttcagattgt aagaatcgat
      661 ggcaggagga tccaggttga agagaaaaaa aaccttgccc ccacacagta agaagaaaga
      721 aatgattggc attcaagtat tcgaccaaat tttgcgcact gcaccggcta gcttgcgtcg
      781 gccgatcttg gcagcagcac atcagctttt gggagcctta cagcagagtg aggtacgctt
      841 ccaaagcgtg tttgagaata ctgtcagagg catggctctc ctgaactcca caggagagat
      901 tatggaaagc aatctggctc tccacaacat actgggttat agcggagaag aactaaaggg
      961 aaaaacccta atcccataca ttcatcctgg agatgcggag ctggacccca cattgaatca
     1021 agccctgtct gctcacgaag cgggagatta tgaggatgaa aagcgctttg ttcgtaaaga
     1081 cggacaagta cgttggggtc gatggattgt ttctcatgtt tatctagagc acggacaaaa
     1141 tccggcgttt gctttagcca tgctggaaga tatcaccgaa aggaaaaaag atcagcagtt
     1201 cctggtacag tcggagagtc tatctatcgt cggtcgattg ggcgcctcac tggcgcatga
     1261 aatcaataat cccttgcaag cagctcttgg atgtctggga cttgctgagg aagcgttgga
     1321 agaaggagcc gacgtacggc gctatctggc gatcgcgatg gaggaattag agcgggcagc
     1381 ggggattgtc tcacgattga gaaaccttac gcggacgcca aaggagaaaa aaaaacaacc
     1441 tgttgatctc aatgctctca tagagcaatc tcttgtgctg attcgcaaac ggtgccagga
     1501 ccaggctatc gaagtaaagt ggaggcgcaa gtccgatcta tctccggtct cgattgttgc
     1561 ggacaataat cggcaagtat ttctaaacct catgctcaat gctgtagaag caatgccgga
     1621 tggaggacag ttaaagattt ccactatttc gacaggcagg cctcggggtg ttcgggttac
     1681 aatttcagat accggcaaag ggattgaacc tgactcactg tccaatattt ttgaaccatt
     1741 ctatacgacc cggccggatg gcatgggctt ggggctctac ataagcaaaa agatcgtgga
     1801 ggaccaaggt ggtcgtcttg aggtagagag tcgcatcggc aagggttcca agttcagcgt
     1861 ctggttacct ggctgagaag gaaagaatga ctaaatcact gcgcgcatta gtcgtggatg
     1921 atgaagaagg tgtacgcttc tttctaatag agaccctcca acgtatggga catgttgtga
     1981 cggaagcctc cagcggcgaa gaagctttgg atcacctgag agacacagcg tttgatcttg
     2041 ccatcctgga tctgaaactg ggcggtcctg ccgatggtca gcgagtatta gaatcgatcc
     2101 gctggcgttg gcctgccact atggtcatta tgctgacggc acatggctca ttagaatcgg
     2161 cggtagaagc cattcacgaa ggtgtagatg gttacctact caagcccgtg cgccctatcg
     2221 atgtgcggcg ggctgtacaa aaggcgctcc accgccgcca aaagctaatt gtcgctgagc
     2281 cggagcccct agaccatcgg ctgcaacgcg gacctttcca tatcgatttg gaacgctatg
     2341 aagcaaaatt cgaaggacag atactgagtc tttcgccacg tgattttgcc ctcctacatt
     2401 atttgatgca gaaagcccca caggtggtca gcgctgcaga gctcgttaag gtgatacgcc
     2461 agtatgagcc agagtacgcg ggcgaggcgc gcaccatgat taagtggtat gttcatcagt
     2521 tacgaaaaaa ggtggagcct aatcccgcca agccgcgtta cattctcaac gtgcgcggtg
     2581 ttggatatcg tttcgtcggg tgaatttgga gaacgaatgc tttgaggctg tacttccctc
     2641 ctgtttggcg gtagaaaatt gagtggagta gcagattaag ctggactctt aacgagcaag
     2701 tccaaccaaa aaccaactct ttccaaccag agtcaaaggc aaccctaccc gaatccgtcg
     2761 gtttgcggtt gctttttctg ttaataatta gtgcagcgtc cgcaacagtg cggatgcgag
     2821 aaaaacagaa tccaggaaga ggaggatctg tatgcaggga ccttatcgca tactcgttgt
     2881 cgatgatgac ccaaaggtct tattcatgct gcgcgagtcc ctggaaaaac tgggaaatgg
     2941 ttatgggatc gcgaccgctg ccgacggaca acaagcatta gtcaaggcgc aaaaccagcc
     3001 cttcgatctg atcatctctg atgtaaaaat gcccggtatc gacggtatac agcttgtaga
     3061 aatgatccgt tctttgaaca ttgaaacgat gctgatctgg atcactgcct tgggatgtga
     3121 aagcctgaag gctgaatgcg aacgattgag tgtatcgagc tgtctgcaaa aaccactgcg
     3181 gatcagcgag atccgggatg cagtacaggc agcattatcg tctaaagatg aaaataggca
     3241 ggggcatgga aatgaaagga gatccgaaaa tgattagaat ggaattgaag gaaggaacta
     3301 gtgttttcac gactggtggg aaagaggtgg gtaagatcaa tcgatttgtt ctggatccga
     3361 caacaaacga agtcactcac atcgtcgtcc aaaaaggatg gcttttttca gaggacaaag
     3421 ttgtaccatt tggtatggtt aactctgcca caatggaaag agtggtgttg agtgagaata
     3481 ttgacacatt cgaccaattg cctccgtttg aggagaggca ctatgtcaag gcaggtgagt
     3541 cagagggaag tgattcacac gatcagcaat cagatcaaga tgaaataagg aaggttgacg
     3601 tacgtcgcga gggccggcta cgagatgatg attttccatc ctattattgg tatccgccgc
     3661 atggatatgt gggttatccg attggcgttt atggttggcc gtcgctggaa acgactcgca
     3721 acattcccgg gaatacaatc tcactaaagg aaggaacgga tgtggtgagc tctgatggaa
     3781 agcatgtcgg tgatattgag cgcctgtttg tcgcgtctga ctcaaataag gtcacacatt
     3841 ttgtcatctc gcaaggcttg ctcttcaaag accgcaagct tatcccggct cattgggttc
     3901 gtactgctga tgaaggaaag gttgaccttg ccgtctccac tcaactgctc aaagaccttc
     3961 caacgtatga gtgatcgata ggattccagg taagtctcaa acagcgcagc aacacgatga
     4021 cgagtgaatt tgttgaaatc gggatgtgaa atcgaatatc aaattctgac ctgaggtgat
     4081 tgtggtcaat caactgcctg gctcagcagt ttgaacgggc agacagtagt ttaggaaaag
     4141 gacaaaggtg gagccagaca tgaacgcact gaacattatc gcagaccata gcctggacgg
     4201 gaagatttat cgggtcacga gcgcgttact ggcttctgaa ctgctcttgg actggcaagg
     4261 aaaattcttc gttgtctcgc tgatatttag ccagatagat aagcattggg attgagagtg
     4321 tgagtatgtt tctacattgt taccctaaaa caggaaggag atcaacatga aaactgtcat
     4381 tgctgccgcc ttgggcgaat gtgtccatgt tgcaggtgta tccaattttt tgaggctagc
     4441 cgaaatggcg ggctggcgca ctgtattttt aggacccgca gtctcagtgg aacgcgttct
     4501 cgaagcggcc cgcgctgaga aggctgaact cattggcgtg tcctatcgtc tcacccctga
     4561 aacaggagag cggttacttg gtgactttgc cgaagaagct gctgacttgc acgaggctgg
     4621 agtcaaattt gcctttggcg gcactccacc cattgccgaa cgggccagca agctggggtt
     4681 ttttgagaaa atttttgatg ggagcgaatc tcctgagcta gtgctggagt atctcaaagg
     4741 tcagtcaaca agtgacatgg ttgagtcagg ctatcctcaa agtgttaccc aacgaattgc
     4801 atggcgatcg ccctatccga tattgcgcca ccacttcggc ttaccttcca tggaagaaac
     4861 tatagatgga attaagaaga ttgcggaagc gggagccctt gacctgatat ccctcggcac
     4921 tgatcaggac gcacaggaaa acttctttca tcctgaaaga caggatccac gtcgcaaagg
     4981 tgcaggtgga gttccagtac gcagtgccga ggattatcgt atgctgtttg aagccagtcg
     5041 cgttggaaat ttcccaatgc ttcgagcgta ctctggcacc gatgatttca tacggtatgc
     5101 cgaggtattg accgaaacga tccataatgc ctggtgtgct attccactgt tctggttcaa
     5161 tcagatggat gggcgcggtc cctgggattt ggaaggttca atccgcgaac atcaaaaggt
     5221 gatggcctgg tatggagaac gaaatattcc tgttgaactc aacgaaccac atcactgggg
     5281 catgcgagac gcgtcagatg tgatctatgt ggttagcgca tttcttgcgg cctacaacgc
     5341 aaagaaattt ggggtgagtg attacattgc acaaatgatg tttaatagcc cacctggtac
     5401 tgcggatgcc atggacctgg caaagatgct ggccgttctt gagctgatca atccgcttgc
     5461 tgacgaagac ttccgcatct ggaagcaaac acgcattggc ctgctttctc atcccctgga
     5521 ttcagatgct gctcgtggcc atctggcggc gagtacctac ctgcaaatgg cgctcaaacc
     5581 acatatttat cacatcgtag gtcatactga agcacatcat gccgcgacgg ctgatgatgt
     5641 catcgcagcc agtaggatcg tccggcgtgc gatcacgaat gcactgggcg cgcctgatat
     5701 gacccaggca aaggaagtgc aacagagaaa aaagaaacta atcagcgagg ttagatttac
     5761 ccttggagta attcgcgacc tggcagatat aggtgtagat gatccatggg cagatccggc
     5821 cacattaact aaagccgtga ggaccggggt cttggatgcg ccccagttat taaacaataa
     5881 atttggatgc ggtgtgattc gaacgcggat tatcgatggt gcgtgtgagg ctgtagatcc
     5941 gtcgggacat atcatctcag agaaagaacg actatccaaa taggaagaat caaaatattc
     6001 cagggcaaag gagcaaacat gacagaaaaa tatacagtaa ttggtgcggg gcacggtggg
     6061 aaagcaatgg cggctcatct ggcattgatg ggcctggatg ttgcactctg gaaccgcaat
     6121 tttgaccata tttcgatcat caagcagcga gggggaatcg agctggaaag ctttgatggt
     6181 gggcctcatg gctttggaaa cctgagcctg gttacacctg atctcgagca agcggttgcg
     6241 catgcacaga tcatcatggt ggtcctgcca tcctcagcac atgcaggcat ggcgaaggcg
     6301 gtggcaccct acctcaggga tgggcaaatt gttgttctgc atccagggcg cacgcttggc
     6361 gctttggagt ttacaaaagt tattcgcgac aacggttgta ccgcggacgt aacggttgca
     6421 gaggctgaaa cgttcatcta tgccagccgc gccgagggac cggcccaggc gcgcatcttt
     6481 cgcattaagg aagctgtccc actagccgcg ttgccagcct gccgtacgga aatggtattg
     6541 aatgcaattc agtgcgctta tccacagttc attgatggcg aggatgttct tcacactggc
     6601 ttgaacaata tgggcgcgat cttccaccct gccttaacac tgcttaatgc aggctggatt
     6661 gagcacacac atggagatta tcagttctac atcgatggcg ttaccccgtc ggttgcacgt
     6721 gttcttgaag tgcttgatcg agagcgcgtg acggtcgcgt cgtctgttgg gatccgcgcc
     6781 cgtacctccc tggagtggct taagttggcc tataacacgg ctggtgaaaa tctgcatgaa
     6841 gccattcaca atcagccggg ctaccacgga atcaaggcac cagcaacact caaccaccgc
     6901 tacatatttg aggatgtgcc gatgagcctc gtgccgatcg catctctcgg aaaacgttat
     6961 ggagtgtcag tgcaggggat ggaaagtgtc attcgactgg ggagcatcat tcatcgtacc
     7021 gattactggc gtcgaggtcg aacgttagaa cgccttgagc tggagcggtg gagtgtgagc
     7081 gagctcacgc gttttgtgcg agaaggtatt gttgagtagt cattttccct ttgcagttat
     7141 aaaggcagag ctctgggaaa caagcttaga ttgattgcat cagcatcatc aatcaaatta
     7201 acacgaaagg agataatcat gtgcggaatc gctggggtct ttcaacgacc agcaaatcaa
     7261 attgtcaaat ccatggtgag gaatattgcg catcgtggtc cggatggtct tggcgtcatg
     7321 gatgtgccat atggaacatt tgggcataca cgtcttgcca ttttggatct gtcggagggg
     7381 cgtcaaccaa tggaataccg agacacaacc attgttttca atggcgagat ctataactac
     7441 caggatctcc agcggaaaca ccttacaaat tacaacgtga ggactcgttc ggatactgag
     7501 atcctgctcc acctatacag gaagtttgga ccagaatgtg tagaactgtt agatggtatg
     7561 ttcgccttcg ccattcaaca caacaatgat ctgtttctgg ttcgtgatcc aattggaatc
     7621 aagccacttt attatgggac gagtcatgat ggaaagcatt ttattttcgg ctctgaaatc
     7681 aaggcgctcg atggtgtagt gaacagcata acagaatttc cggcaggaca ttggtttcac
     7741 tctcgccttg gatggcacaa atattttcat cttgaagaaa aaattcgtct gtttcaagga
     7801 accgagcatg atgtgcagcc agtcatcaag tccactttgc gggacgcagt gcataagcgt
     7861 ttgcttgccg atgtgcctgt cggtgtaagt ctaagtggcg ggttggatag tagcattgtc
     7921 acgagcttag cgcgtgagga gtcggaacaa ttgcattcgt ttgcggtggg tgtgatgggc
     7981 agtccagatc ttgaggcggc acggcagatg ggaaagcatc tcgatacaat tcaccatgag
     8041 tatatctaca ccgtgccaga tatgatcgcg gcattgcccc atgttcttta ttacttggaa
     8101 tcgtttgatc cggcgctggt acgtagcgcg atcccaaatt acttccttgc caagctggca
     8161 tctgaccatg taaaggtaat actcactggc gaaggcgccg atgagattta tgccggatac
     8221 gattatttgg ccggattcca atcgccggat gcactgcagg gcgaaatgct ttccatcacg
     8281 aatgcgcttc acaatacgaa tttgcagcgt gccgaccgta tgtccatggc atttggtctg
     8341 gaagcccggg tacctttttt ggatgtgcag tctatatcgc tggcattggg gattccgcca
     8401 tcctggaaga ttcatgctgg aagggcgccc aagtccttgt tacgtcaatc gtttgctgat
     8461 gatttaccaa aagaaattgt taaccgtcca aaggctaaat tttcacaagg agccggatca
     8521 tccgagttgc ttgcacaaca agcagtggaa aaaatatcag atcagcaatt catgagcgaa
     8581 cgcaagcgtc taaaggaaga ttggaactac aaccttcaaa ataaggaagc ccttttctat
     8641 taccggatcc tgcatgagtt ttatgatgac aaatggattc ttcctaccat gggaaatagc
     8701 aggagtctct gagattgatg ctggaaacgt ttgattggta tagctgggtg atattgcctc
     8761 ttatcgtatt tatcgcgcgc gtattggatg tcacccttgg gacaatgcgg atcatcttcc
     8821 tctcgcgcgg caggaaatac cttgcaccca tccttggatt tgtagaggtg ctgatctgga
     8881 tcacagtggt ctctcagatt gtgggaagtg cgcaaaatcc tgtcgcctat attgcctatg
     8941 cagccgggtt tgcgacagga aattacgttg gactttctat cgaggataag ctggccattg
     9001 gcacgctagt gattcggatt attgtaaccc acaatggaaa gagcctgatc aataacttgc
     9061 agggtgcagg ttatggtgtc acctccgtgg atggacaggg agcaacagga ccagtgatcc
     9121 ttgtgtatac agttgtcgtc cggaaagaac tggagcaggt gatcgagatc attgaaggtt
     9181 cttcacccaa atccttcttt actgtggagg aattgcgttc agtgagacag ggtatcttcc
     9241 cgcaacgtcg tagactaccg gatgaaatcc tacttcgcag aaaatcaaaa taagaaccat
     9301 tgacgagtag ccatcgatcg tccctcactc aagtgctacc atgtaaatgg tggttaacga
     9361 ataaacccag atcctgcgaa gcccgtttgg ccgggagaaa acaccaaggc aaatagcgcc
     9421 agcccgcgac ctgaacagag tgatctagaa agtgaagagg caaccttata aacttttcta
     9481 tgtacgatct gactgctttt aacctgaaga atatgacgac actgggcgtc gccctgcgcc
     9541 agatggggat gggggcgtca agtatggagg aagttgccaa taaaattgtg cgatatctct
     9601 atgagaatct cggagacgga actggaaagc caataagtgc gttggtagag ttcttcatca
     9661 cctatcccta tcaaaaactc gaaacggagc tacagcagca cgttcgacat gtgcttggta
     9721 tgcgtcccgc agacccagat ttaagctgcc agactctgct cgccacggtc ggtgaagaac
     9781 cgaattggaa tgatcggcga cgttcggttt attataaagt tctacctctg acagaaggga
     9841 tcgtggatga taccccgatg tttgcccagg tatcggaagt ctttgacatt gtgatgaaaa
     9901 gccgtacgaa acctaatcca gaatcgctgg tggaacttga acagaaaacc tacaatgtct
     9961 tctacgtgcc ggatgccgct gggagcgacc atgttcccga gcaggaagac tttgtgatcc
    10021 gatatggact ccgctcgata ctaggatttt atgcgttgct gccgagcggt aacctcttca
    10081 ctgtgattgt ttattcaaag acacgcattc ccagcgcgac aactgaattc ttcaagactc
    10141 tggcgttgaa tgtgaagatt tccatcctac ccttcgatga tgtcgcggtc ttcgccggat
    10201 caactcccga cgggctgaat actattccgc aaaatgaact catccacttt cgttcgcagg
    10261 cggcaagtct ggcccagctg ttgaaggtcc gcgaccgaat tgtggtgcag caatcagatc
    10321 gcattgagca ggttatggtt gaacttcgtg aacaagcacg agagctggaa cgcaccaatt
    10381 acgaattgga aagcacgatc gctgagaatg tgcgcctgcg acagctggcc gctgaaactg
    10441 cggtaatcga ggagcgtaac cggctctctc gtgatttgca cgattcggtc acccaggcgt
    10501 tgtacggtca gacgctgtat gcagaggcgg ctgctcgtca actggatgcc ggaatggtag
    10561 cgcaggccgc tgagcacctg cggcagttgc gtcatactgc tcaacaggcc ctgcgggaaa
    10621 tgcgtctgct catttttgaa ctacgtccat ctgcgcttga aacggaaggt tttgcggctg
    10681 cgttacaagc gcgcctcgaa tctgttgaga tccgcactgg actggaagcg aatgtgaaca
    10741 tcgacgagga actccacttg accccagaga tggaaaatga tctgtattgg atagtgcagg
    10801 aggccttaaa caataccctg aaatctgcag aagcgaaaaa agtggtggta tccttatcgc
    10861 gggagtccaa ccagatgatc ctccaaattg cagatgatgg caaagggttc gatcccgaaa
    10921 tatcttccaa tgcgcgtggg cttggtcttc gatcgatgga ggaacggtca gaaaaactag
    10981 gtgggcattt ccatatcgag agccaacctg gcaccgggac agttgtccgt gtggaggtgc
    11041 cctttgggtg aaccaattcg gatcttcatc gccgatgacc atgccattgt tcgctctggg
    11101 cttcgggcgc ttctggcgac cgaaccggag atcgaagttg tgggagatgc agccaacggt
    11161 gagcaggcag tgaccggcgt caaagagcgg cagccagatg tggttttaat ggacctggaa
    11221 atgcctggga tggacggcat tgaggctatt cggcaaatca ttggcgccga tccggaagca
    11281 cgcatcctgg tgctgaccgg ttttgccaca gatgagcgcg tctttcccgc cattcaagcg
    11341 ggagcgttgg gctatatctt gaaggatgcc gggcccgagg ctctggttca ggccatccgc
    11401 caggtgtatc gcggcgaatc atcgctgcat cccaagatcg cgcgcaaact gatcgaggaa
    11461 atggcgcgcc cggtggacca accgcctact gccgatcccc tcactcctcg cgaagtagaa
    11521 gtgttgcagc tggtggcaaa gggaatgagc aatcaggaaa tcgcgggaaa gctcgtcatt
    11581 ggcgaagcaa cggtgcgatc acatgtcaac aacatactgc gaaaattaca cctcgcgagc
    11641 cgcacacagg ctgccttata tgcattgcgc gaaggatatg catccctcgg agattcgtag
    11701 agatatttgc tcgcctcctg atcctccaac aaatgttggg gatggctcca caaattgtgt
    11761 aatgggaccg ctgcagagcg gtcctttttg ttgacggaaa gagtcgcctt tctgtggatg
    11821 tgagaaacgc actgtgttcc tataatcctt ttatcgcaaa ggattcggtg tgaacagtgt
    11881 acgagcagaa ccaaccggaa gagagaaagt ctctacgctt gctcattgtc gatgacaacg
    11941 cctctgcccg ctcggggatg aaagcgttgt tggaaaccgt cgcgagccac aaatggaagc
    12001 cggatatttt cgaggcggcc agtggaccgg aagcgatcga cctcgttgaa acacttcagc
    12061 ctgaccttat tctcatggac gcccacatgc ccaccttcaa tgggatcgag gcgacgcgca
    12121 taatcaaaca gcggtggcca accatcaaag tgataatact cacgctttat gatgcttatc
    12181 gagacatagc tttggcggct ggagccgatt tattcctgct caaaggtggt tcgtcagaag
    12241 aattgttttc tgcaatttat aacgtatcta tcgggcaaga caaatcttaa ttctttaagt
    12301 acgaggagaa aaatgagccg acgcaagaaa tctgtgaaca cagcggtcat cgtcgccgtg
    12361 ctccttgtgc tgtttatggg tgcaagggca aaacaggacg atgagttaac atacgctggc
    12421 tcgcagctcg aaggagcaac ctatagcgaa cgtggtccgt atacggttgg agtacgcgac
    12481 ttgatcatcc agggtaaaag acctttggaa atctcgctct ggtatccggc tgcaaatggg
    12541 gaaggtggag aggaggtaac tcaatatcct tatgaaatca aaatggccaa gccaatcgga
    12601 aagtttgccg tggcgacgtt tgctggacga gcgttgcgtg atgtgtcccc tgatctctcc
    12661 gacgttcctt atccgctggt tatcctatcg ccgggctttt ccctggggtc attcgcatat
    12721 gcctggctgg cggaacatct tgcttcatat ggatttgtag tgatcgctcc tgaacatcag
    12781 gaacatctcg atcctgccaa tgaattatgg cgatctgcca tcgagcgccc acaggatgtt
    12841 ttgagcgtgc tcgcctacgt ggatgaacaa acagcggatg gtggagtttt tgatgggctc
    12901 atcaacgccg agcgtatcgc tgtgatcggt cattcctacg gcggatacac ctcgctggca
    12961 tcggcagggg cgcggataaa cacgccggca ttcaagtcgc attgtgagga agcctccgca
    13021 tcgaatcacc ctgccgcctg gttatgcaat gaaattttgc cgcatcttga cgacatggct
    13081 gaccttgccg ggctggatga gacgccaaat ggtctttggc ctgcatggac tgactctcgc
    13141 atagtggcca tcgtaccgat ggcaggagat gctttcttct tcggtcagga aggattggct
    13201 gagatcaaca tacctgtcat ggcaatcggc ggcaccaaag ataacgacgc gccttatgaa
    13261 tggggaactc tccctgcttt tgaacatgct tccagcccga gaaaagtgct gatctccctc
    13321 acggatgccg agcacatgat cttcacaaat ccgtgtgaaa agattccgtt ttacctgaag
    13381 ctcttctcac gtgaattttg ccacgacaca gattgggatc gcaaatacgc ccacgaactc
    13441 gtcagtcact tcacggcggc gtttctcctc gcggaactga agcaggattt ggccgcatcc
    13501 caggcactgg cgcccaacaa tattgaactc cccggcatga agtacgaagc gcagggatat
    13561 tagcggagca ggataatgtc agaaagaaat gatcttaaaa ccaatccaga acagccgatg
    13621 atctatcaga tcaagatcaa gggacatcta ggaccgcaat ggcaggatcg gtttgacgga
    13681 aggccgccct gcacgtattg ctaaagaatg tgcgcgatct gggaatgagc ctggtcgtag
    13741 tcaaccgcat catttcagac cacggagttg ctttggaaat caaaggtgaa gaagatacat
    13801 catccttaaa tatagaaaat cattacaaaa ggagtatgga aatgagctca aactcgttgg
    13861 ctttagaaaa aaataagatg acaaacaccg caggtgcaac gtcccagctt aacgtcggaa
    13921 ggatcgcagg tttgctctac ctaaccatca ttgtcagcgg tattttcgct gagttttttg
    13981 tccgtcagag tctgcgcgtg cccggcgatg cagcagcgac cgccagcaat attatggctt
    14041 ccgcgtcgtt attccgcctc ggcattgctg ctgatctcgt catgatcatg tccgatgtgg
    14101 cattggcatt gattttttat gtattgctta aaaaggttaa ccagtcattg tccctgctcg
    14161 cggcgttctt tcgattaggg caagccacaa ttttgggact taacctgttg aatcttttcg
    14221 ctgtgaaaca gcttttgagt ggagagagct ttctgtccac acttgaggcg gggcagttaa
    14281 attcgctggc gtacctgttc ttcaacgcgc atggcaccgg ctattccatc gccatggtat
    14341 ttttcgggtt cagcatcctt gtacttgggt atctgatctt caagtcaggt tacatcccca
    14401 aagtattggg agcgatgttg atgctggcat cggtgggtta tctggtggac agcttcgcaa
    14461 aatttctgat gccgaactac gcgagttacg aaggcgtgtt tacgatggtc gtgttcgtgc
    14521 ccgccttcat ctcggagttg gcactgtgcc tgtggctgtt gatcaaaggc ttgaaagtcc
    14581 agcctcaaga aataaactaa tggagtagaa gaaatgaaat ccatccaaaa aaccgccaga
    14641 tttgcgggag tactttatct gatcattacc gtagctgcca ttgtggctca tcaatatgtc
    14701 ccatccatgt tgatcgtgcc tggagatgcc gcaacgaccg ccagtaatat tgcagcctcc
    14761 gagacattat tccgtatcgg tggcgtgggt agtgagttga tcatcctgct gagtgagatt
    14821 gtactggcag tggtcttgta tgtattgctc aagcccgtca acaaaacgct gtctttgatc
    14881 gccgcagttt cccgtttggc gatgaccacc attcatggtc tcaacctgtt gaactattat
    14941 ttcgttctgt ccctcgtggg tgacaccggt tatgtagctg cattcgcgcc ggagcaggtc
    15001 aatgctcttg tgtcgttgtt cctggacgca catcattatg gatttaccat cggcatcgca
    15061 tttctcacca tccatgtctt tgttctgggg tatctgatcc tcaagtctgg atacttccca
    15121 agcgttttgg gaatcctgtt tctggctgca ggactgggct atttggtaga tagcttcgcg
    15181 cttctgctct cgactagtta tgatacgaca ccgatcttcc ttgccatccc cattgctttg
    15241 gcggaaattg cctttccgtt ttggcttttg acaaaaggca taaacgtcga acagtggagg
    15301 aaaagcgtcc ttgccccagc ctgaattgca tttatgctga agatggggcc tgctttcaag
    15361 ctgtccccga gcagggacgg ccggaaattg aaatcatttg aaattgccgg aatccgcaac
    15421 gtcttgaaaa gcaccaaaaa ggagaacaga tgaccaggtc cttagaagag cagcgcgaag
    15481 aatttgccag gagaagattt atagcgatgc cgattgccgg aattattgca tggtctatcg
    15541 ccgggatctc aggcgcattc ctgccgaaaa tatatgcggt atgggtgtta tttatctcga
    15601 ccggttccat tgtgtatcta ggtattttga tttcaaaatt taccggtgaa aattttttag
    15661 acaagaaaaa ggataaaaat gagtttgata cactttttat ctatacagtc ggaatggctg
    15721 ttcttgttta ctcgattgca attccatttt ttctggttga ttatgaatca ttacctctga
    15781 cggtcggcat acttgccggg ttgatgtggg tgccgttttc ctggattgtt gggcactggg
    15841 tcggtatttt ccatacgctg ttacgcttgg tattggttgt cgcgttttgg tatttattgc
    15901 cagattcgag atttgtagcc atacctgtgg tgataattgc agtatatttc gcgacaattg
    15961 ttattttgga aaatcgttgg cgcagattag aaactggctg acgctatccg taaacgaatg
    16021 gttcacacaa aggataagta aatgaaagca atcatctgca caaaatcggg ccctcccgaa
    16081 gttcttcaac tcagagaagt ggagaggcct gttccaaggg acaatgaagt tttaatccga
    16141 gtgcatgcgt ccactgtcac acagggtgat gtgatgcttc gaaaactcca ccctttattg
    16201 tttttaccga tgcgattgtt cggtgtaaaa caaaaaagga caccgggtca cgagttcgct
    16261 ggggtggtgg atggcgtggg gaaagccgtg aagcgcttca ggaagggtga ggcggtgttt
    16321 ggcacgacca ctggattgag tgtgggtgcg aacgctgagt atgtatgcct gcctgaagaa
    16381 tggtccgacg gcgtactcgc cctcaaaccg tcaaatgcga cttttgaaga agctgccgcc
    16441 ctcccagtcg ggggaatgac cgcgttgtac attctgcggc aagccaatat tcgacgcgga
    16501 cagaaagtcc tggtgtatgg cgcctcaggg agtgtcggca cgtatgcagt tcagattgcc
    16561 aggcagtatt ttggggcgaa agtgaccgga gtatgcagca ccagaaatat cgatttagta
    16621 agagcattgg gtgctgagat ggtcatcgat tacacgagag aagatattac cagcagcggt
    16681 gagacctatg atgtcatttt cgatgcagtg ggcaagctct catcctcgaa gacgagaaac
    16741 atgctaaagg aaaatgtaac atttcttacg gttcaatcct cgaccactga agagcttgag
    16801 aatctcatcg ttcttagaaa cctgatggag gaagagaaac tcaaaccggt gatcgataaa
    16861 tgttattcgc tcgagcaggc cgcagaggcg catcggtatg tcgaagcagg gcacaaaaaa
    16921 ggcaatgtgg ttatcagcgt ggggtactcc gtcaattgac aataagcagg caggcgggtg
    16981 tacgcctgat gttggtatcg cacacatggg ttctggcgcg aaaagcagaa aatcagtttt
    17041 ttatgaaaag acatttaata ataatttgtc tcacattgat cggtagttct tgtgcccgag
    17101 aa
//
LOCUS       DASTJP010000713         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_378533, whole genome shotgun sequence.
ACCESSION   DASTJP010000713 DASTJP010000000
VERSION     DASTJP010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378533"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..831
                     /locus_tag="VFQ23_20850"
     CDS             244..831
                     /locus_tag="VFQ23_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9909110.1"
                     /translation="MHRRIFHGNINPNDIAHALMAEFNRGNMHAQLLGNKDKVAVQIA
                     TRRGTMSGGHTALTINIHKVEDGIMAEIGQQAWLGLAASLGHTAISAFRNPLNLLSRL
                     DDLAQDIENIQLSEKVWKIVDRTVATLGASHALSDRLTRVACEYCNSANPLGEANCLA
                     CGAPLGDVHPTTCFNCGFVVTTNEKKCPNCNQNLR"
     gene            950..1822
                     /locus_tag="VFQ23_20855"
     CDS             950..1822
                     /locus_tag="VFQ23_20855"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9909111.1"
                     /translation="MKLRLDQLEANLQKLVEDQLSGLLPGLKLEDRVIQHLATALREN
                     VIQQKDESAIAPDTFTLIVATDTSPMWKEQRIIEALKNIIATAGKDVGLRFTTQPTIN
                     ITTDDTFSADEVRVVAAHKLEPVAETQGMQTSLYNEDHGEGENIPENAFLIIEGVKVH
                     QLSDSVVNIGRRLENQLVIDDPRVSRNHAQLRAIKGRFVLFDLNSTGGTFVNGQRTSQ
                     TVLYPGDVISLAGVALIFGQDNPPPRLDLNDPASGETEPGASERPTATIDQNTVDIKT
                     DSLKFRFREEKPPK"
     gene            1819..2280
                     /locus_tag="VFQ23_20860"
     CDS             1819..2280
                     /locus_tag="VFQ23_20860"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9909112.1"
                     /translation="MIATVVLVLRFALAIALYAFLGWALFTLLQELKQQGNKLSTQKK
                     PSITIFVNVDRKRESQRYFTQTEIIIGRDTHCDLSVMDEALSAHHARITHHHGQWWLE
                     DLNSRNGTFLNREKLNTSAVIITGDQFKCGNTLFDIRVDADEPTPSSTTQT"
BASE COUNT          672 a          597 c          547 g          562 t
ORIGIN      
        1 gcccgccccg cacgttgatc tgttcagcat aaatgacagt cgccaggacg ccggtcggtg
       61 tggctgaatc ctgaaaggct tcagcctgtt tcggcgaaag aaaaacacct attcctaatg
      121 ttgcaagtac cgctattcca agtagacttt taaaccaaaa tcttcgcatt gcgtagataa
      181 tcataagaca taaaacacga cgacaaatcg tcgtcgctac taaagcggat tataatcgaa
      241 aacatgcatc gacgaatttt tcacggaaat atcaacccaa acgacatcgc acatgctctt
      301 atggcagaat ttaatcgggg gaatatgcat gcccaactgc tcggaaacaa ggataaagtt
      361 gctgtgcaaa tcgccacacg ccggggaacc atgtccggag ggcatactgc cctgacgatt
      421 aacatccaca aggtggaaga tggaatcatg gctgaaatcg gccagcaggc ctggctgggc
      481 ttggcagcga gcctcggtca tacagcgatt tctgcctttc gaaaccccct aaatctgctg
      541 agcaggctgg atgatcttgc acaggacatc gagaatatac agctgagcga gaaagtatgg
      601 aaaatcgttg atcggacagt tgcgactctg ggcgcttcac atgcgctttc agatcggctc
      661 acccgcgttg cctgcgagta ttgcaattcc gccaacccct tgggagaagc caattgcctt
      721 gcctgcggcg ctcccttagg agacgttcac cctacgacct gcttcaattg tggctttgtc
      781 gtcactacca atgaaaaaaa atgccccaat tgcaaccaaa atttgcgata accaatagac
      841 gtgttgtacg ttaatatgtt atattgaagc tgcaagattg taatcttgca gatttttcac
      901 gtcgcctcta taatatttga aactcacaaa ttgggtttga gggctttcta tgaagctgag
      961 acttgaccaa ttggaagcaa atttacaaaa acttgtcgaa gaccaattat cggggctact
     1021 acctggtctg aaactggaag accgcgtcat tcaacacctc gctactgcgc tacgagaaaa
     1081 cgtcatccag caaaaagatg aaagcgcaat cgcgccggac acatttactc tgatcgtcgc
     1141 caccgacacc tctcccatgt ggaaggaaca acgcatcatc gaggcgctaa agaatatcat
     1201 cgcgactgcc ggaaaagatg tggggctacg gttcaccaca caaccaacga taaacattac
     1261 aaccgatgac actttttctg ccgatgaagt aagagtcgtc gccgctcata aactcgaacc
     1321 tgtcgccgaa acccagggta tgcaaacttc cctttacaac gaggaccacg gggaaggcga
     1381 aaatattcct gaaaatgcct ttctcatcat tgaaggtgtt aaagtgcatc aattatctga
     1441 ttcggttgtc aacatcgggc gcaggcttga aaatcagctg gtcattgatg atccgcgggt
     1501 ttcccgaaac catgcacaac tgcgtgcaat caaggggcga tttgttctgt ttgatctaaa
     1561 ttcgaccggc ggtaccttcg tcaacggcca gcggacgagt cagactgtgc tctatccggg
     1621 cgatgtgatt tcgctggcgg gcgtcgcctt gatctttggg caggataatc ccccgccgcg
     1681 gctggatctt aacgaccctg catctggcga aactgaacct ggtgcaagtg aaagaccaac
     1741 cgcgaccatt gaccagaata cagtggacat caaaactgat agtcttaagt ttagattcag
     1801 agaagaaaaa cctcctaaat gatcgcgaca gtagtcctgg tcttgcgatt tgccttggca
     1861 attgcgcttt atgcatttct ggggtgggca ctttttacac ttttgcagga actcaaacag
     1921 caggggaaca aattatcaac tcaaaaaaaa ccaagcatta ctatttttgt taatgttgac
     1981 cggaagcggg aaagtcaacg ttacttcact cagaccgaaa tcatcattgg cagggatacc
     2041 cattgtgacc tctccgtgat ggatgaggcg ctctctgccc atcatgctcg tatcacacat
     2101 caccacggcc aatggtggct ggaggatctg aactccagaa atggaacctt tctcaaccgc
     2161 gaaaaattaa acacctccgc cgttattatc accggtgatc aattcaagtg tggaaacact
     2221 ctcttcgaca ttcgcgtgga cgcggatgaa cccacccctt cgagtacaac tcaaacatag
     2281 aggttagtca tgcctgatca agtgatcctt gcaatgattg gaggctcggg gctttatgaa
     2341 atgcccgggc ttaagaatat ccaggaatac aacatcaa
//
LOCUS       DASTJP010000714         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1575607, whole genome shotgun sequence.
ACCESSION   DASTJP010000714 DASTJP010000000
VERSION     DASTJP010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575607"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFQ23_20865"
     CDS             complement(<1..876)
                     /locus_tag="VFQ23_20865"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET9909113.1"
                     /translation="MEDKIITYGGQAVIEGVMMRGRKALAIAMRAPDGQIVIHQENLG
                     AVYRSRLAKIPFLRGVILLWDALGLGMRALTLSANVQTGEDEKLEGPALYLTLGISLA
                     IGIGLFFLLPAGIGGFAESSLGWNPWLNNLLEGVIRLILLVAYIWAIGFMPDVKRLFG
                     YHGAEHKTINAYEAGAELTPETVAKFPIEHARCGTAFMLTLVLVSILVFTALGPMSMF
                     WRLASRVLLIPFLAGIAVEYIRWTANHLDSPIVRFLIKPNLALQALTTREPDTSMLEV
                     AIESFKTMRKAEQELV"
     gene            complement(883..2262)
                     /locus_tag="VFQ23_20870"
     CDS             complement(883..2262)
                     /locus_tag="VFQ23_20870"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET9909114.1"
                     /translation="MKEARQTALDYARQNNERFVDELKEFLAIPSISTSPENNADVRR
                     GAEWVAEQLRALGMDNVQVMTTRGHPVVYGENLKAGKDAPTVLIYGHYDVQPVDPLEL
                     WTTDPFKAEVRGDNLYARGSSDMKGQVIASFKAVEAVARTSGLPVNVKWLIEGEEEIG
                     SINLGDFIKKNKALLKADFCINPDAGLINKDSPTITYGLRGLAYFEIRVYGPDRDLHS
                     GLFGGTVHNPAQALAELIAGMHDKKGRITLPGFYDNVRKSSKAERAEIARLPTKPKHF
                     LKQTNVPALWGEPNYTPEERVSIRPTLEVNGLLSGFTGEGSKTVLPAWAMAKISCRLV
                     PDQEPEEIHKTMVKYMKKHAPKTIRWEVKNLHNSGTAITERDSAGVEAMSKGLEQAWG
                     KRPFFKREGGSIGVVVQLQKYAGVESVLTGFGLPEDNAHSPNEKLHLPTWRKGIESFI
                     HFFYNIGKR"
     gene            complement(2275..3966)
                     /locus_tag="VFQ23_20875"
     CDS             complement(2275..3966)
                     /locus_tag="VFQ23_20875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9909115.1"
                     /translation="MKQLRWQILVVIVTLGIVAVLLLSQQAPSGGLPILPQPEQGGVY
                     TEGLVGSLGRLNPLLDWNNSADRDVDRLIFSGLLRFDEHGLPQKDLAEGWGVMPDGTV
                     YNFTIRPNALWHDGEPVTSDDIIFTIDMMKSEGSLYPPDIKELWNKIEVTRLDDKNLK
                     FTLPEPYVPFIDYLTFGVLPKHLLESVQPAQMASADFNIKPVGTGPYRFDHLLVDDGQ
                     IIGVVLTISPNYYGTPPYVEQVVFRYYPTSADAFDAYKQGDVLSVSQITNDILSGALE
                     EPNLSVYTSRLPQMSMVLLNLNNTEVAFLQDAKVRRALMLALNRSYIVNSFLEGQAIL
                     ADGPILPGSWAYYDGIEHFDYNPDEAVNLLKSEGYVIPAGGGDVRTKDGIPLVFTMLH
                     PDDAVHTQIAQTMQEYWAAIGVRADLQAVPYDQLVLDSLASRTYQAAFVDLNLSRTPD
                     PDPYPFWHQAEAVGGQNYTQWDNRAASEYLEQARVTTDYTLRTRLYRNFQVVFGKELP
                     ALPLYAPVYSYGVDVEVQGVQISALYDSSDRLATFNQWYLLTRRALEQETPTTVP"
BASE COUNT         1023 a         1087 c          985 g          962 t
ORIGIN      
        1 gactagttcc tgctctgctt tgcgcatcgt cttgaacgat tcaatggcaa cttccagcat
       61 ggatgtgtcg ggctcgcggg tcgtcagcgc ctgcagggca agattcggct tgatcaggaa
      121 acgcacaatg ggcgaatcca ggtgattggc cgtccagcgg atgtattcga cagcaatccc
      181 tgccaggaac ggaatcagca acacgcggct tgccagcctc cagaacatgg acattggtcc
      241 gagggctgta aacaccaata tcgaaaccaa tacaagtgtc aacataaagg cagtgccaca
      301 gcgggcgtgc tctatcggga atttcgcaac ggtttcaggt gtgagttccg ctccagcttc
      361 ataggcattg atggtcttat gctccgctcc gtgatagcca aacagacgtt tcacgtcggg
      421 catgaagcca atcgcccaga tataagcaac cagcaaaatc aaacgtatca ccccttccag
      481 caggttgtta agccacggat tccagccaag agagctttca gcaaatccgc caataccagc
      541 cggaagcagg aaaaacaaac cgataccgat tgccagagaa attcccaggg tgagatacag
      601 agcaggaccc tcaagttttt catcttcacc tgtctgcacg tttgcagaga gcgtcaacgc
      661 tcgcattcca agcccgagcg catcccatag caaaatcacg ccgcgcagaa atgggatttt
      721 tgcaagccgc gaacgataaa ctgccccaag gttctcctgg tggatgacaa tttgtccatc
      781 tggcgcccgc atggcgatgg caagggcttt tcgcccacgc atcatgacac cttcgataac
      841 ggcttgaccg ccgtaggtaa tgatcttatc ttccatgaat aattaacgct ttccaatgtt
      901 ataaaagaaa tggataaagg actcaatgcc ttttctccag gtaggtagat gcagtttttc
      961 gtttggtgaa tgggcattgt cttctggtaa gccaaaccca gtgagaacag attcaacgcc
     1021 tgcatacttt tgtaattgaa caaccacacc gatcgagcca ccctcgcgtt tgaagaacgg
     1081 tcgtttgccc caggcttgct ccagcccttt cgacatggct tcgacgcccg cgctatcgcg
     1141 ttccgtaatg gctgtcccgg aattatgcaa attcttaact tcccagcgga tcgttttggg
     1201 cgcatgtttc ttcatgtatt tgaccatcgt cttatggatt tcctcaggtt cctggtctgg
     1261 tacaaggcgg cacgaaattt tcgccattgc ccaggcaggc aaaacagtct tggacccttc
     1321 tccagtaaaa cccgaaagta aaccgttgac ttcgagagtc gggcggatgc tcacgcgttc
     1381 ctccggtgtg taattgggct caccccacaa cgccgggaca tttgtctgtt tgagaaagtg
     1441 ttttggcttc gttggaagcc gagcaatttc ggcgcgttct gctttgctgg atttgcgaac
     1501 gttatcataa aaacccggta gagtgattcg gcccttttta tcatgcatac ctgcaatcaa
     1561 ctctgctaag gcctgcgcag ggttatgcac tgtaccacca aacaagcccg aatgaaggtc
     1621 acgatccggg ccatatacac gaatttcaaa gtaagcaagc ccgcgcaatc cataggtgat
     1681 ggtaggtgaa tccttgttga tcaaacccgc atccgggtta atacaaaaat ctgccttgag
     1741 caatgcttta ttcttcttga tgaaatcacc caaatttatc gaaccaattt cctcctcccc
     1801 ttcgatcaac catttgacat tcaccggaag gccactcgtt cgcgccacag cttcgaccgc
     1861 cttgaacgac gcaataacct gtcccttcat gtcagacgaa ccacgggcat acaaattatc
     1921 accgcgcact tctgctttga agggatctgt cgtccacaac tcaagtgggt caacaggttg
     1981 aacgtcataa tgaccataga tcaaaaccgt aggtgcatcc ttccccgctt tgagattttc
     2041 gccgtacaca acgggatgac cacgggttgt catcacctgg acgttatcca taccgagtgc
     2101 acggagttgt tccgccaccc attccgcgcc acgccggacg tctgcgttat tttcagggct
     2161 ggtggaaatt gatgggatcg ccagaaactc ttttaactca tccacgaatc gctcgttatt
     2221 ctggcgagcg taatcgagtg cagtttgcct tgcctccttc atcttgctga ctccttaagg
     2281 tacagtggtg ggtgtctctt gttcgagagc gcgccgggta agtagatacc attgattgaa
     2341 cgtggcaagg cgatcactcg aatcatacag tgcagagatt tgcactccct gtacttctac
     2401 atcgactcca tatgaataca ccggcgcata gagcggaagt gcgggcaatt ccttcccaaa
     2461 caccacctga aagttgcggt acagacgcgt gcggagggtg taatctgtgg tgacgcgcgc
     2521 ctgttcgagg tattcgctgg cggcgcggtt atcccattgt gtgtaatttt gtccgcccac
     2581 cgcttccgcc tgatgccaga acggataagg atccgggtca ggtgtgcgcg aaagattcaa
     2641 gtccacgaag gcagcctgat acgtgcgtga agccagggaa tccaaaacaa gctgatcata
     2701 gggcacagcc tgcaagtccg cgcggacgcc gatcgccgcc cagtactctt gcatcgtttg
     2761 ggcgatctgt gtgtgcaccg catcatccgg gtgaagcata gtaaatacta aaggaatgcc
     2821 atccttggtt cgtacatcgc cgccacccgc cggaatcaca tacccttcgc ttttgagcag
     2881 gttgaccgct tcatccggat tgtaatcaaa atgctcgatg ccatcgtagt aggcccacga
     2941 gcccggcagg atgggtccgt cagcgaggat ggcctgtccc tctaaaaatg agttgacaat
     3001 gtaggatcga ttcaacgcca gcatcaacgc ccggcgaact ttcgcatcct gtaaaaaggc
     3061 gacctcggta ttattcaaat ttagcaggac catactcatt tgcggcaggc ggctggtgta
     3121 aacagaaagg ttgggttctt ccagtgcgcc gctcaatata tcgttggtga tttggctcac
     3181 ggaaagaaca tccccttgtt tataggcatc gaacgcatcc gcagacgtcg gataataccg
     3241 aaacacaacc tgttctacgt aaggcggcgt cccataataa ttcggagata tggttaatac
     3301 cacaccgata atctgaccat catccaccag caaatgatcg aaacgatagg gccctgtacc
     3361 gacgggcttg atattgaaat cggcacttgc catttgcgct ggctgaacag actcaagtaa
     3421 atgcttgggt aggacgccaa acgtcaggta atcaatgaac ggaacataag gctcaggcag
     3481 ggtgaacttc agattcttat catcgagtcg tgtgacttcg atcttattcc aaagttcctt
     3541 gatatccggt ggatacaaag aaccctcgct cttcatcatg tcgatagtga aaatgatatc
     3601 gtcactggtg accggctcac catcatgcca gagcgcgttg ggtcgaatcg taaagttata
     3661 aaccgtaccg tcaggcataa ctccccaacc ttcggcaagg tctttttgag gcagaccatg
     3721 ctcgtcaaag cgcaggagtc cactgaaaat cagacgatcc acatcgcgat cagcagagtt
     3781 gttccaatcc aatagtggat taagacgccc cagcgagcca accaaacctt ccgtgtagac
     3841 acctccctgc tctggttgcg gcaggattgg aaggccaccc gagggcgcct gttgactcaa
     3901 tagcagcact gccacaatac cgagtgttac gataacaacc agtatctgcc agcgtaattg
     3961 tttcataggg tataaaaaaa gaaaggctcg attacccccg cggtatttgc cgcgcggaag
     4021 taacggcctt tcagaaggga ttcaccagat tacggag
//
LOCUS       DASTJP010000715         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_709997, whole genome shotgun sequence.
ACCESSION   DASTJP010000715 DASTJP010000000
VERSION     DASTJP010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709997"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFQ23_20880"
     CDS             <1..231
                     /locus_tag="VFQ23_20880"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909116.1"
                     /translation="MFVRPQFRGLGLGKLMLDRLAEYAQERQVNILRLETGIYQTDAI
                     RLYERYGFLRRAPFGEYKIDPLSVYFEKQLEK"
     gene            300..989
                     /locus_tag="VFQ23_20885"
     CDS             300..989
                     /locus_tag="VFQ23_20885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET9909117.1"
                     /translation="MSASATFQLITFDVYTALFDIEGSLIPVIQALLNEKVEAVDFVR
                     IWRQKQLEYALISNSLQRERIPFAVITRLGLDYALNRVQVDLTDASRQSLVSEWNRLK
                     LWPEAAQVLVEVNNRGYAIGLLSNGDETMLQTLAAKLPIQCDHIFSSEKAAYYKPHPG
                     VYKLPLKTLKLAANQVLHVAGSATDVTGTKAAGLHCAWSNRKHDRVLDSRYQADYEFE
                     NLLGLMDVLQV"
     gene            1043..1525
                     /locus_tag="VFQ23_20890"
     CDS             1043..1525
                     /locus_tag="VFQ23_20890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909118.1"
                     /translation="MNFTFRPLDINSAGAIITWHYEPPYDIYDMASTDPEDTLQYLLD
                     PQNAFHGMYGQSNELEAFCSFGADGQVTGGDYSVPAFDIGLGIRPDLTGQGRGSEFVS
                     AVIDFADQTYTPDRLRVTISAFNRRAQRVWQKAGFQVIQKFQGGWANMDFVVMTKSRS
                     "
     gene            1654..>2177
                     /locus_tag="VFQ23_20895"
     CDS             1654..>2177
                     /locus_tag="VFQ23_20895"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9909119.1"
                     /translation="MNYNQPYEAKTYSKLMQSVCRFVLLSIWLVGCTVEPAVLPTATS
                     LPTVAAPTQGNLNQETPTSAPPTITPTTLSDPERQIMTQRLIQAAEAGEISTVTELLQ
                     AGADINGRDEQGRTAAMAATHANQVEMVRVLIEAGADINIQDDRLDNPFLYAGAEGLL
                     DILKLTIDAQADPR"
BASE COUNT          574 a          508 c          530 g          565 t
ORIGIN      
        1 atgttcgtcc gcccgcagtt tcgcggttta ggtttgggca aactgatgct ggatcgcctg
       61 gcggaatatg cccaggaacg ccaggtaaat attttgcgat tggagacagg catttatcaa
      121 actgatgcaa ttcggctgta tgaaagatat ggctttcttc ggcgcgcccc cttcggggaa
      181 tataaaattg atccgctcag cgtctatttt gaaaagcagt tggagaaatg attttgtcat
      241 tgcgaggcag cctgaagggc cgacgaagca atctccgttc acctctaaaa aaagctcata
      301 tgtcagcctc agctactttt caattgatca ccttcgacgt ttatacagca ctcttcgaca
      361 tcgaaggtag tcttattcca gtcattcagg cattactcaa cgaaaaggta gaagccgttg
      421 attttgttcg catatggcgt cagaagcagt tggaatacgc gttgatcagc aactctcttc
      481 aacgtgaacg gatacccttt gcagtgatca cccgcctggg cctggattat gcattgaacc
      541 gggtgcaagt cgatctgaca gatgcctcgc gtcaatctct tgtttcagaa tggaatcgac
      601 tgaaactgtg gcccgaagcc gcgcaggtac ttgttgaagt caataatcgt ggatacgcaa
      661 ttggattgct ctccaacggg gatgagacga tgttacaaac gttggccgca aaactgccga
      721 ttcaatgtga tcatatcttt tcttctgaaa aggctgcata ctacaaacca catcctggcg
      781 tatacaaatt gcccctgaaa accttgaagt tggcagcgaa tcaggttctc catgttgcgg
      841 gatcagcaac agacgtcaca ggtacgaagg cggcaggatt gcattgtgca tggtcaaatc
      901 gaaaacatga tcgggttttg gatagtcgtt atcaggcaga ttatgagttc gagaatttgc
      961 tcggtttaat ggatgtactc caggtttgat cgcgaacaga acttcgtttc ataccctttg
     1021 aacctgtaat acttttgtgt tcatgaactt cacgtttcgt cccctggata taaacagcgc
     1081 cggagcgatc atcacctggc actacgagcc accatatgat atttatgata tggcgtccac
     1141 agatcccgaa gacactttgc aatacttgct tgaccctcaa aatgccttcc atggcatgta
     1201 tgggcagagt aatgaactcg aagccttttg ctctttcggt gcagatgggc aggtaaccgg
     1261 aggtgattac agtgtgcctg catttgatat tggattgggg attcgcccag acttaaccgg
     1321 acagggacgt ggatcagaat ttgtaagtgc cgtgatcgat tttgcagatc aaacgtatac
     1381 accggatcgg ctacgtgtca ccatttcagc cttcaatcgg cgcgcgcaaa gagtgtggca
     1441 aaaggccggc tttcaagtta tccaaaaatt tcaaggcgga tgggcaaaca tggattttgt
     1501 tgtaatgacg aagagcaggt catagcattg tagtgataac gaaaaagctt cagaataggc
     1561 atcgcgaaat tttcatcttc gccagctgtt gtggtttggg tcaactccga taattcccgt
     1621 aatattatcg atagtttcat catctacttg tgaatgaatt acaatcaacc gtatgaggca
     1681 aaaacgtatt caaaattaat gcaatccgtt tgtaggtttg tccttttgtc aatttggttg
     1741 gtggggtgca cagttgaacc cgccgtctta cctacggcaa cgagcctgcc aacagtggct
     1801 gcgcctacgc aagggaacct gaatcaggaa acaccgactt ctgcgccacc aaccattaca
     1861 cccacgactt tatctgaccc tgaaagacaa attatgactc aaagactcat tcaggctgcc
     1921 gaagctggcg aaatctcaac tgtcacagag ctcttacaag ccggcgccga catcaatggg
     1981 cgtgatgaac aagggcgcac cgccgcaatg gctgccacac atgccaatca ggtagaaatg
     2041 gtccgagtgt taattgaggc gggcgcggat atcaacatcc aggatgaccg cctggataat
     2101 ccctttctct atgccggcgc agaagggttg ctcgatatcc ttaagctgac gattgacgcg
     2161 caggcagatc cccggtt
//
LOCUS       DASTJP010000716         7619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_906848, whole genome shotgun sequence.
ACCESSION   DASTJP010000716 DASTJP010000000
VERSION     DASTJP010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906848"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFQ23_20900"
     CDS             <1..585
                     /locus_tag="VFQ23_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909120.1"
                     /translation="LNNDIVIDLNSPQPTTATAAFYDSHPVTTNMNNLVSFYPFTRSL
                     SATGAVEGVTVTPLVQTNERSWGETNFEDLQQGGQVGFDEGIETLGPLTLAMAGEHAT
                     TGGRVVVFGTSLFATDQIFDQYGNGDMFVNSVDWSAEQEDIASITPKTPTARTFTPPN
                     QLQWIAILLGSVLIIPGLVVFAGISAWLTRRRQG"
     gene            592..1173
                     /locus_tag="VFQ23_20905"
     CDS             592..1173
                     /locus_tag="VFQ23_20905"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET9909121.1"
                     /translation="MRRPTVIFLLLFLALAATYYYLNNREQPADIALTVEPTTEVTYL
                     FPAEDGLPTSIRIESNAGDAVEVARDTDNAWALIQPQAAPADQASAEAAASQVTTMQI
                     LDTIPDVDPEIIGLADPQYIITIKFTGGVERKVDIGVVTPTESGYYVRNTNGDIVIIS
                     RSSVDALLGLLLNPPYLETPTPEATPSNATPTP"
     gene            1299..2510
                     /locus_tag="VFQ23_20910"
     CDS             1299..2510
                     /locus_tag="VFQ23_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9909122.1"
                     /translation="MDVDKILMVDEEEEFSAIARLIELGRQKGYITLDDILHFFPEAE
                     QDVEQLEEAFSALLSAGIPFVEDTATAEPTEDELVAVEATEAEPEVDLGLDDYLANID
                     TDDTIGLYLKEVSRVPLLTAEEEVKLAQRIERGRMSREELAKGNASPRRRSELRKLIE
                     DGWAGREHLITANSRLVISVAKKYMGRGVPFLDLIQEGNIGLIRATKKFDYRRGHKFS
                     TYATWWIRQAVTRAIADQGRTIRVPVHMGDQINKLLRVQHQLTQRLGREPSVEELAIA
                     LDVPPKKVENMIQVARRPLSLETPTDDEEDSVLGDFIEDDEAPPPDDTATYNLLKEHL
                     GEVLNGLPPREVRILQLRYGLLDGQAYTLEEVGRKMGVTRERVRQIEAQALSRLRHPS
                     IRRKLRDYLGE"
     gene            2569..4578
                     /locus_tag="VFQ23_20915"
     CDS             2569..4578
                     /locus_tag="VFQ23_20915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9909123.1"
                     /translation="MKQQRVLPAIAILAWSGFVIAAYFVVQKPLALQVMKPLITSIWG
                     LVVFAVLLLNALALGVLTLRRFVGNLLNGPAVLALACGIGLGELGLLGFLLAATGASD
                     FIVLLGVQALILGWSISTGKFRSVVSLMKEVSKSLLSFFRGLPFWMRWAVFLALTFTF
                     LMTLLPPADAFDALLYHLTIPELWLADGGIQLHNFPHYWFPGLIEGIYLWGLGLGSEI
                     VPQQVHFAFALCSLILLCDWTRRLWGELASGWAVMLFISMPNILLLASWAYTDLALIF
                     FGLGMLYTLSMGKELNDTRWWRISAVSAGMAMGVKYTSFFMPVTAVLLIAAWNFQKKR
                     ELLEQVFNFTLISTLTGLIWYLRNWFWMGNPIYPFLLGGRFWDEFRAAWYANAGTGIG
                     WDIGALILLPLTVTLGYQDVNGIDADIGPLLLLTLPLALWAVTSQRHAEQPQKISLAT
                     ISLFSLIGMVFWVYGYISTRNLWQARLLLPAIVPFLIITAVGVTSLSSLDSRRLRISF
                     IVSTIVAASIFVNLLDMGLILLSRNPLAIATGIVTKESYLSRYQPGYADALRIISQLS
                     PQSKTYFLFEPRSYGMHRAVQPDAILDNFSHDIFLHKTPEDIISAWQAEGYTHVLVNK
                     RNAEAILENRGEGPLLDEVTGSLKLVSVSQDENYELFEIPFGASK"
     gene            complement(4539..5771)
                     /locus_tag="VFQ23_20920"
     CDS             complement(4539..5771)
                     /locus_tag="VFQ23_20920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET9909124.1"
                     /translation="MKLPVSEIFRQTDIQKSSGTKRLDFSMLSAWLMMSVTLAVIAFV
                     VFGVDFRGYYAASRVLISGENPYDYRAVAQVLLDVTGEMGNNPYYYPPWFAWLFIPLV
                     YLPFQVARAVWMILNVIIWSLGLWQLGRIIGWPQPGWRRYLLFTVTTFSFAWITWRYE
                     QAAILVFAMWVALISSIYNEKWQQTGIWLALLLVKPNVTLIVIAGVTLWLLRQGRRRT
                     VGIMLFTIVVLLSISTLITPNWFQPFFEDGFGRGLTVALDGPDEIVALRINTTFLDWM
                     RTLGVEAPWSVVLYGVTIGISIFVFFRSVYFSRSFLELICILLLLSYAITPYSLQYDF
                     PPLVIPLYWALSQCISSPKKFAIGITLAAFVCSVIFWQQNISWAYWMIIGLIALLIWA
                     VVGGKSYLEAPNGISNNS"
     gene            6054..7508
                     /locus_tag="VFQ23_20925"
     CDS             6054..7508
                     /locus_tag="VFQ23_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909125.1"
                     /translation="MKKILLPGFTDFLFIMLFVSALVTGPKMLNIDGDLPRHLLMGKY
                     VLETGSPPTQEIFAYPYEGQSYVSHEWLAGVLFYLSYVLFDLNGVVLLASLLIAGTFT
                     ILFSDAVRQNGEIVPNFLLIFFGASVTSIHWIPRPHLFTMLFLAIWLTRIERLDRGNS
                     SKLWMFPALMLLWGNIHAEFVAGFLTLLAYIAGWLWNYLFARQSLNASTGKNLLLVTI
                     TSFVATLINPAGLRTWGTILGYINNSYLMSRITETRPPDFARAEYFPLLLLLVISVLL
                     MVTRRDKFTPAHIFLLLGFGTMSLLSARNAHLFGVVAPFVLSNGLKGLSNIQPFKRME
                     SLISGIERQISGRMVPSLLAVVISVPILASAVNFNRFEPAVFPVDAVHWLKENPQPGQ
                     VFNAFDWGGYILFHLWPEKKVFIESQTDVRGELTREYEKVITLQYDWQGIFQRYDVSW
                     VIIPPQWDLATELQRIGWEIAYQDPTTIILVAGN"
BASE COUNT         1959 a         1816 c         1850 g         1994 t
ORIGIN      
        1 ctcaacaacg acatcgtcat tgacttgaat agccctcaac ctacgacagc cacagccgcg
       61 ttttatgatt cgcatccggt cacgaccaat atgaacaatc tcgtttcctt ttatccattt
      121 acgagaagcc tgagtgcaac cggcgccgta gaaggtgtga ctgtgacccc cctggttcaa
      181 accaatgaac ggtcctgggg tgaaacaaat tttgaggatc tgcagcaagg tggacaagtt
      241 ggattcgatg agggtatcga aacgttggga ccgctaaccc tggccatggc tggcgagcac
      301 gccactaccg gtgggcgtgt ggttgtgttt ggaacatccc tttttgctac cgatcagata
      361 tttgatcaat acggcaatgg tgacatgttc gtcaattcgg tggattggtc tgctgaacag
      421 gaagacatcg ccagcatcac tcccaaaact ccgactgcga gaactttcac ccctcccaat
      481 cagcttcaat ggattgctat tttacttggc tccgttctga ttattccagg gctcgtcgta
      541 tttgctggaa tttccgcatg gcttactcga aggcgtcagg gatagaacaa aatgcgtcgt
      601 cctaccgtca tatttttgct gctcttcctt gcattggcag cgacctatta ctatctcaat
      661 aatcgagaac agcccgcaga tattgcactg accgtagaac ctactaccga ggttacttat
      721 ttattcccgg cagaggatgg tctaccgacc agcatccgaa tcgagtcgaa cgcgggagat
      781 gctgtcgagg tggcgagaga tacagataac gcctgggcat tgattcagcc acaggccgcc
      841 cctgcagatc aggcctccgc cgaagcggca gccagtcagg tcaccaccat gcaaattctg
      901 gatacgatcc ctgacgtaga ccctgagatt attggcttgg ccgacccgca atacatcatc
      961 acgatcaaat tcacaggtgg tgtggaacga aaggtcgata tcggtgttgt tacaccaaca
     1021 gagagcggtt actacgtccg caatacaaat ggtgatattg tcatcatcag ccgaagttcg
     1081 gtggatgcat tacttggact cctcctcaac ccaccttatt tggagacgcc gacacccgaa
     1141 gccacgcctt ctaacgcgac tcctacaccc tagaacgtaa catttcctta tttgaggcgt
     1201 ctaactacag tagaaaagca atgaattgag tattgaaatt caccctaaaa aagtgtatcc
     1261 ttatagtgta ttctaaagag aaaatggacc tttaacgaat ggatgtagac aagatattga
     1321 tggttgatga agaggaagaa ttttccgcta ttgcgcggct gattgaactg ggccggcaaa
     1381 agggatatat cacgctggat gatatattgc atttcttccc tgaagctgaa caagatgttg
     1441 aacaattgga agaggctttt tctgctctgt tgagcgcggg tattcccttt gtggaagaca
     1501 ccgctacggc agaacccacg gaggacgaac ttgtcgcggt ggaagccacg gaagcggaac
     1561 cagaagttga ccttgggctg gatgactacc tggcaaatat tgatacggac gacacaatcg
     1621 gcttatatct taaagaggtt agccgcgtac cgctgttgac cgcggaagaa gaggtcaagc
     1681 tcgcccagcg catcgagcgc ggacgcatgt cccgcgagga actggcgaaa ggcaatgcca
     1741 gcccgcgtcg tcgttctgag cttcgcaaac tgattgaaga cggctgggcc gggcgcgaac
     1801 atctgattac tgcgaactct cgtctggtaa tttcggtggc gaaaaaatat atggggcgcg
     1861 gtgttccgtt tctggatctc attcaggaag gcaacatcgg cttgatccgc gcgacgaaga
     1921 aattcgatta ccgtcgcggc cacaaattct caacctatgc cacctggtgg attcgccagg
     1981 cggtcacgcg cgcgattgct gatcagggac ggacgattcg tgtccccgtt cacatgggcg
     2041 atcagatcaa caagctttta cgtgtacagc accagttgac ccagcgcctg ggtcgtgagc
     2101 cgagcgttga agagctggcc attgcattgg acgttccacc caagaaggtg gagaacatga
     2161 ttcaggtggc gcgacgtccg ctctcgcttg aaacaccgac cgatgacgaa gaagattccg
     2221 tcctgggaga tttcatcgaa gatgatgaag ctccgcctcc ggacgacacc gccacatata
     2281 acctcttgaa agaacacctt ggtgaggtgt tgaacggatt gcctcctcgc gaggttcgta
     2341 tccttcaatt gcgttatggg ttgctcgatg gacaagccta cactcttgaa gaagtgggac
     2401 gcaagatggg ggtcacacgc gaacgagtaa gacagattga ggcacaggca ttgagtcgac
     2461 tcaggcaccc ctcgatccgg cgcaaactgc gcgattacct gggcgaatag aactttaaaa
     2521 acgacaccaa ttggtgtcgt ttttgattgt cagcttataa ttcgattgat gaaacagcaa
     2581 agggtcctgc ccgctattgc catcctggca tggagtggtt ttgtaatagc ggcatacttc
     2641 gtggttcaaa agcctcttgc actgcaagtg atgaaaccac tgatcacgtc catatggggg
     2701 ctagttgtgt tcgcagtgct tttgctgaat gcactggcac tcggggttct cacactccgt
     2761 cggtttgttg gcaacctcct gaatggccct gcagtgcttg ccctcgcatg tggaattgga
     2821 ttgggggagc ttggcttgct cggcttcctc ctggctgcta caggagcttc cgactttata
     2881 gtcctcttag gcgtccaggc actgattctt ggttggtcaa tatcgacagg aaaatttcga
     2941 agcgtcgtat cgctgatgaa agaggttagc aaatcgcttc tgtctttctt ccgcggactg
     3001 ccgttttgga tgcggtgggc agtgtttcta gctttaacat tcacgttctt aatgacgttg
     3061 ctccctccgg cagatgcatt tgatgctttg ctatatcacc tgaccattcc cgaactttgg
     3121 cttgcagacg gcggcataca actccacaat tttcctcact attggttccc cggtttaata
     3181 gaaggtattt atctctgggg actgggcttg ggatccgaaa tcgttccgca acaggttcat
     3241 tttgcttttg ccttgtgctc ccttattctg ctctgcgact ggactcgacg cctttgggga
     3301 gagctggcat ccgggtgggc agtcatgctg tttatttcga tgccgaatat tttattgctg
     3361 gcgagctggg catacacaga tttagccctg atcttttttg ggctgggaat gttatacaca
     3421 ctctcgatgg ggaaagaatt aaacgatacc aggtggtgga ggattagtgc cgtcagcgca
     3481 ggaatggcaa tgggcgtcaa atacaccagc ttctttatgc cagtcacagc tgtactgctc
     3541 atcgctgcct ggaattttca gaaaaaaaga gagctcctcg agcaggtttt taattttacg
     3601 ttgatcagca cattaacagg tcttatctgg tacttgcgca attggttctg gatgggaaat
     3661 ccgatctatc cgtttctgct tggaggacgt ttctgggatg agttccgcgc ggcatggtac
     3721 gcaaatgcag gaacaggcat cgggtgggat atcggagcac tgattctatt gccgctgacc
     3781 gtaacgttag ggtatcagga tgtaaatggc attgatgcag atattggacc cttgctcctt
     3841 ttgactttgc cgttggcctt gtgggcagtg acgagccaac gacatgctga gcagcctcaa
     3901 aagataagcc ttgccacgat ttcgctcttc agccttatag ggatggtttt ctgggtttac
     3961 gggtatatct caacgagaaa tctgtggcag gcccgtcttc tgcttccggc aattgttcca
     4021 tttctgatca tcacagcagt gggcgttacc tcgttgagca gtctggattc gagacggctt
     4081 cgcatttctt ttattgtttc aacgattgtg gccgcgtcaa tctttgtcaa tctcttggac
     4141 atgggtctta tacttctttc tcgaaatccc ctggcaattg caacgggcat tgttacaaaa
     4201 gagagttatc tttcgagata ccagccgggc tatgcagatg ctctacgaat aatatctcag
     4261 ttgtcaccgc aatcgaaaac ttattttttg ttcgaaccaa gaagctacgg tatgcatcga
     4321 gcggttcaac cagatgccat actggacaac ttttcacatg acattttctt gcataagacg
     4381 ccagaagata tcatttctgc atggcaggca gaaggctata cacatgttct cgtaaataag
     4441 agaaatgcag aagcaatttt ggaaaacagg ggtgaagggc cccttttgga tgaggttact
     4501 ggctcactca aacttgtatc ggtttctcag gatgaaaatt atgaattatt tgagatccca
     4561 tttggagctt ccaagtagct tttacctccc acaaccgccc atatgagcag cgcaattagc
     4621 ccaataatca tccaatatgc ccatgaaatg ttttgctgcc agaatattac actacaaaca
     4681 aatgctgcca gcgtaatccc gatcgcgaat ttcttcggcg aggatataca ttgagaaagc
     4741 gcccaataca acggaattac taggggagga aaatcgtatt gcaaactata tggggtaatg
     4801 gcataagaga ggagcagcaa aatacaaatt aactcaagga acgatcgaga aaaatagacg
     4861 ctcctgaaaa acacaaatat actgatcccg attgttactc cataaagcac cacactccag
     4921 ggagcttcga cacccagagt tctcatccaa tcgaggaagg ttgtgttgat gcgtaaggct
     4981 acaatttcat ccggcccgtc aagcgcaacc gtcaatcccc ggccgaaccc gtcttcaaaa
     5041 aaaggctgaa accaattggg ggtgatcagg gtggaaatag aaagtagaac aacgatagta
     5101 aacagcataa tacctaccgt gcggcgccgt ccctgacgta agagccacag agtgaccccc
     5161 gctatcacaa tcaaagtgac attcggtttg accagcaata gagccagcca aatacctgtc
     5221 tgctgccact tctcgttgta gatagacgaa atcaatgcga cccacatggc gaagacgaga
     5281 atcgctgcct gttcataccg ccatgtgatc catgcaaatg agaaagttgt cactgtaaaa
     5341 agtagatacc gcctccagcc aggttgcggc cagccaataa ttcttcccaa ttgccacaaa
     5401 ccaagactcc agatgatcac attcaatatc atccaaacag cgcgtgcaac ctgaaacggc
     5461 agatacacga gcgggatgaa aagccaggca aaccatggcg gatagtaata cgggttatta
     5521 cccatttcac ccgtcacatc caacaaaacc tgtgcaactg cacgataatc gtatggattt
     5581 tcaccggata tcaaaacccg cgacgcggcg taatatccgc ggaagtcaac gccaaagact
     5641 acaaatgcaa tgacagccag ggtaacactc atcattaacc atgccgagag catggaaaaa
     5701 tccagccgct tggtccctga agatttttgg atgtccgtct ggcggaatat ctcactgacc
     5761 ggtaatttca tataattcca tatcttgatg gctgtagatg tgtaagtccg gatgccagca
     5821 aagtgaattc aagattcaga ccaggtaggt tgatctttcg atgaattatt cattcgaatt
     5881 aaagcaatgc cgcgccagca ataaaaatac acctttgtaa attaccgtgc aagaattgca
     5941 tcgagcaatt gtacaagtat ccgggaaagc aggatactca caaaaccctt acaaagcttt
     6001 gatgaagcat atccaaaaac ccagtacaaa tagttatgat ttagaataaa tcaatgaaaa
     6061 aaatcctgtt gcccggcttt acggatttcc ttttcatcat gttgtttgtc agtgctttgg
     6121 tgaccggtcc gaagatgctc aacattgacg gagatctgcc gcgtcatctg cttatgggaa
     6181 aatacgtact cgaaaccgga tcacctccga cgcaggagat atttgcctat ccttatgagg
     6241 gccaatccta tgtctcacac gaatggctcg caggtgttct tttctatttg tcctatgtgc
     6301 tgtttgactt gaacggcgtc gtcttactgg caagtctatt gatcgccggg acatttacga
     6361 tcttattttc tgacgcggtg agacaaaatg gggaaattgt tccaaacttt ctattgatct
     6421 tttttggtgc gtcggttaca tcgattcatt ggatcccgcg tcctcacctg tttacgatgc
     6481 ttttcctggc gatatggcta acccgaattg agcgcctcga tcgaggaaat tcatcaaagc
     6541 tctggatgtt tccggcactg atgcttttgt ggggaaacat ccacgccgaa tttgtggctg
     6601 gttttcttac gttattagca tacattgctg gttggttgtg gaactatctt tttgcacgtc
     6661 aaagcctgaa cgcatccact ggaaaaaatt tactgctcgt tacgattact tcatttgtgg
     6721 caactttgat taaccctgcc gggctcagaa cctgggggac cattctgggt tacataaaca
     6781 actcgtattt gatgagccgt attacagaaa ctcgtccgcc tgattttgct cgggcggagt
     6841 acttcccatt gcttttattg ctggtgattt ccgttctctt gatggtaaca cgacgggata
     6901 aattcactcc tgcgcatatt ttcctcctgt taggtttcgg cacgatgagt ctgctttctg
     6961 ccaggaacgc tcacctgttt ggtgtagttg caccattcgt gctttcaaac ggtctgaaag
     7021 ggctttcaaa tatccagcca ttcaaaagga tggaatcgct gattagtggc atcgagcgtc
     7081 agattagtgg caggatggta ccgagccttc ttgctgtagt aatcagcgtt ccaattctcg
     7141 caagtgcagt aaatttcaat cgatttgagc cggctgtttt ccccgtcgat gccgtgcatt
     7201 ggctgaaaga gaatccgcag cctggtcagg tgttcaatgc ctttgattgg ggaggctata
     7261 ttttgtttca cttatggccc gagaaaaaag tgttcattga gagtcaaaca gacgtcagag
     7321 gggagcttac gcgggagtat gaaaaggtca tcacacttca atatgactgg cagggaatct
     7381 ttcaacgata tgatgtttcg tgggtcatca tcccgcctca atgggacctg gcgacagaac
     7441 tgcaacgcat tggatgggaa atcgcctatc aggatccaac gaccattatt ctagttgcag
     7501 gtaattaatc caattgtgaa attagcacac cgtcgctttc ataaacaatt ctgaaatcat
     7561 tgctctcccg aaggctctca acaaaatagg ggaaacttct tggagttgtg accggatta
//
LOCUS       DASTJP010000717        10653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1118571, whole genome shotgun sequence.
ACCESSION   DASTJP010000717 DASTJP010000000
VERSION     DASTJP010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118571"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="VFQ23_20930"
     CDS             complement(<1..1366)
                     /locus_tag="VFQ23_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909126.1"
                     /translation="MMSNSTSQAPKPGQRIPTAPDFPIKWENPREAKITWMSNPAYKT
                     PVPPLIFSVVGTFMVGAGVGFEQAGLDMEMHLKRINTYFYMGMVPKALPPEAVMKAMG
                     LVNRAAPGVFKLMMGKVVAGMSKQQDAALNPIIERFDAHWNDELLPEMKQHIAYFESC
                     DLRGMSLDQLRAHLSETIKRVERMAALHGVIMPMLYAMSQFEELYCELFEGATTLDAL
                     RLTQGFDNKTMEGDRALWRLSRAARSIPEVSEILSKHAAGEVIPALEKSAASQQFLAD
                     LHKWLAYYGQRLNSVFALDQPSWIEDPTPAIQNLQAYISMPNLRPEMEPAALAAEREK
                     AVAEARAKLAGYPQPIVARFETLLKAAQVATVVHEDHNFWIDQRCFYHVRRVIMEFGG
                     RLAQAGALDTVNDVLYLTLDELQSGQDISMKRLVQERKAEMERFSHVTPPPMLGTAPA
                     FEMTD"
     gene            complement(1578..4013)
                     /locus_tag="VFQ23_20935"
     CDS             complement(1578..4013)
                     /locus_tag="VFQ23_20935"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9909127.1"
                     /translation="MPNTIPLSMTLLLGREHEIRTLSQLLRSSEVRLVTITGPGGVGK
                     TSLALQVAQDIQDAFTDGVYFISLATISDPTLIIPTIAHALGVIESPKRLLLDNLKEF
                     LRGKQALLLLDNFEQIMSAAPLLSELLSACAGLRMLATSREALRLRGEQEFPLSPLAL
                     PDQPSLETLMQFSGIALFVQRAQAIQPDFKLTQANAPAVSEICARLDGLPLAIELAAA
                     RIKLLPPQSLLTQLQESSLTLLTSGAKDLPARQQTLRNAIQWSYDLLNADEQRTFRSL
                     AVFVGGCTLQAALAGLEQKTALDTLDSLVNKSLLRQIETDAEPRLTMLETIREFGLEQ
                     LIHTHELESARRAHANYYLSFVEEAESHLTGADQKAWIKRLEREQDNLRAALHWAIEH
                     QQGELAQRMAGALQPFWLTRGYWSEGRRWLEESLAIDLGAALAPAVRAKALYGAGTLS
                     RFQGDFARARMLLEQSLALYRPLADNTGLLMALVELTRITAFQEDQTAKQVFLSEAAS
                     LLETLPDTVMKAYAYTELATVMLDVSINSIQLPPEASRYMAESVRIYRALNNPAGLAL
                     SLARQGSGALFTGDYTLMASQLDEAERLALELGDERILSRLAGIRVLRHIQEGDLTTA
                     RRRLEDTFQRGANRGDHQLPMWLPMLAVVLNRQGLGVWSARVLGLVDTLTGARQAYGE
                     GMAAAFKRFLGIGDIHAELRAQLGDEAFAREFAAGKHLTLDDLRTIPHPPEPNAVAQA
                     ISASETSLTVREIEVLRLLVQDLSNPQIAERLVVSRRTVDAHLRSIYDKLGVKSRDAA
                     IRVAGEQGLISNH"
     gene            complement(4195..5352)
                     /locus_tag="VFQ23_20940"
     CDS             complement(4195..5352)
                     /locus_tag="VFQ23_20940"
                     /inference="COORDINATES: protein motif:HMM:NF022307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HET9909128.1"
                     /translation="MSTPTQHGYLVIADISGYTSFVAKTELEHSQEILSELLGLLVNR
                     FQPIMTISKLEGDAVFAYASEDVFACSDTLIDFVESMYVAFRDKQLSIKRATTCTCNA
                     CRHIPSLDLKFFIHCGDYIQQSIANIKELVGSDVNLVHRLTKNHVTEATGWRAYMLFT
                     EQCVNHNGLKLADAQVQMEAYESLGEIKTFNIDLHTRYQEICEARCIVLTEQDADFVF
                     QMDFSTPPAVTWEWIHNPEKRNLWGGVSDSWSVGDRPQGRSGVGASNHCAHGNSVSTQ
                     VVVDWHPFEYYTIHSYEHGKHTFTETVHFEALPNGGTHVWRAMRMHIPMPRFIRRPAV
                     KFVLITLNHFDKMMVRAAQLAHEEFTKFTGDDFAISSKSLPILPQEGEKER"
     gene            complement(5367..5807)
                     /locus_tag="VFQ23_20945"
     CDS             complement(5367..5807)
                     /locus_tag="VFQ23_20945"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9909129.1"
                     /translation="MEAYELNTVINRPIEEVFTVLSNLENDLKWRTEWVDAKKVSEGP
                     IGMGTEFSLVAKAFGKRIETIYETIQYEPNRVAAWKAASGPIPLTFRRSVERVEGGTR
                     LTIRYEMELRGFFKLVMPLLAGSVTRQHNGDLRKVKELMESRAL"
     gene            complement(5828..6289)
                     /locus_tag="VFQ23_20950"
     CDS             complement(5828..6289)
                     /locus_tag="VFQ23_20950"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9909130.1"
                     /translation="MGKFILRVFINRSQQDVFDFLSNPVNLSKWNSNFASAAWTSSEA
                     PGPGSTYKVLSKMSGGKNEGLFEITQWDPPHRYGYKSISRLPFPIESIESTITLAPKE
                     HGTQITFESQFGLVGVLKLADGMFRKLAEKGDGHNFDTAKRLLEANEPANR"
     gene            complement(6329..6772)
                     /locus_tag="VFQ23_20955"
     CDS             complement(6329..6772)
                     /locus_tag="VFQ23_20955"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9909131.1"
                     /translation="MQKFTASVFVNRSQQDVFDFLSNPAIKHTWMPMLESAAWTSSGE
                     PGVGSTLGAIMKVAGKETEMRLEITQWDVPNRYGYKIHTVMFPMKAMAHSFRLEPEDG
                     GTRVSQVGDFEVVGLLRFAAGLISKMASKINQNELNTLKQLMEAV"
     gene            complement(6889..7782)
                     /locus_tag="VFQ23_20960"
     CDS             complement(6889..7782)
                     /locus_tag="VFQ23_20960"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909132.1"
                     /translation="MNRSSLQMLAGFAPELSRSTLNMTNDLSELAERKSVKAAIKANW
                     EAYHYCLAQSPNVELSVGRYLTWLMTDLPDHFMNLVVCHQLPLEGLDDLIESTLIRFK
                     SMNIRRLSWLTRADPLSVNINQVLLAHGLKFSDSFAAEMAVDLSLLPGCLPTNPGLRI
                     VRVDDEQTLRQWIHIASVGFRIDEKFEQVWYDFFVDAIFEPRFQTYLALLDGKPVGTS
                     QLFLSEGVAGIYNITCLPEARGQGIGSAITLAPLLRARELGYQIGVLQASLNGYNVYR
                     RLGFQDFGKLSLYLWENDRGS"
     gene            complement(7868..8347)
                     /locus_tag="VFQ23_20965"
     CDS             complement(7868..8347)
                     /locus_tag="VFQ23_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909133.1"
                     /translation="MYNWLVFLHIFFAFLFMLSHGAHAAAMLKFRDEPDPERSLTFFN
                     NVPDIKYVRYLTLAMGLFGFAPAFMTVWWRQWWVWLSVLVFVIVAWVMQQYGAAYYGM
                     IFNAAQRLMEARKTDVDLPAAQKEFDKARHAPNALIVSVVGIVGLAILLWLMRFKPF"
     gene            complement(8423..8920)
                     /locus_tag="VFQ23_20970"
     CDS             complement(8423..8920)
                     /locus_tag="VFQ23_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909134.1"
                     /translation="MSTITSSSPISRNSLLRLSVFAGLILGTADVIIYHWFVQNVLGG
                     VPLITLYQYIASGALGESAFAGGIPTALLGVLFHFIFSIIIAGIFILGVDRIPLLRRY
                     IIPGSLLYGLGVYIVMNMIVTPLSATPPLPTPTIPELIVALIVHILVSGLLLGIIVRR
                     NMRSI"
     gene            complement(8982..9476)
                     /locus_tag="VFQ23_20975"
     CDS             complement(8982..9476)
                     /locus_tag="VFQ23_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909135.1"
                     /translation="MSTPATTFPASRNSLLRFSVVTGLIIGTADMIIYNWLVVNVLFG
                     IPLITFFQYIASGALGESAFAGGVTTAFIGVLFYFLISIVVAGVFILGVDRIPLLRRY
                     IIPGSLLYGFGVFIVMNLIVTPLSATPPVPAPTTPELLLIILDHLLFYGLLLGILVRR
                     NMNK"
     gene            complement(9516..9896)
                     /locus_tag="VFQ23_20980"
     CDS             complement(9516..9896)
                     /locus_tag="VFQ23_20980"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9909136.1"
                     /translation="MNEQLEENKRIVREWHELALQKPEEMVAKHLGPNYRQHNPGSAD
                     GPEAFINTMKWIMQNFPELRMETKRIIAEGDLVAIHSHLILKPGDRGSAVVEIFRLEN
                     GKIVEHWDVAQEIPETSANDNTMF"
     gene            complement(9940..10251)
                     /locus_tag="VFQ23_20985"
     CDS             complement(9940..10251)
                     /locus_tag="VFQ23_20985"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9909137.1"
                     /translation="MPTENEAPIKVIVELHAKSGKRAELKNLLKSVVAKYGSVQPGFL
                     GSLQYEVIDNPDILVEIADWASAEARATAMQQAMNAGAYAPLEDLLAAPFRATVLKQL
                     T"
     gene            complement(10281..>10653)
                     /locus_tag="VFQ23_20990"
     CDS             complement(10281..>10653)
                     /locus_tag="VFQ23_20990"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9909138.1"
                     /translation="MPVEEVTHEVHVSAPERGASAAYGEYAVNINDCRICHGPELNGG
                     PFPDPTITRISPNLTPGGELAFWTEADFINTIRTGKTPSGHELDPKLMPWNEYRNLHD
                     YELQAIWLYLQSLPKLLQYTE"
BASE COUNT         2559 a         2705 c         2776 g         2613 t
ORIGIN      
        1 catcggtcat ttcaaaggct ggtgcggtac ccaacatcgg cggcggggtc acgtggctga
       61 acctttccat ttctgctttg cgctcctgaa ccaggcgttt catcgaaata tcctgaccac
      121 tctgcagttc gtccaacgtc agatataaca catcgttcac ggtatccaac gccccagctt
      181 gtgccagacg ccctccaaat tccatgatga cccgccggac gtgatagaag cagcgctggt
      241 cgatccagaa attgtgatct tcatgcacaa cggttgcgac ttgtgccgcc ttcaacaaag
      301 tttcgaagcg ggcgacgatt ggctgtgggt aaccggcgag tttggcacgc gcctccgcca
      361 ccgctttttc acgctcagca gccagtgcgg cgggttccat ttcaggtcgc agattgggca
      421 tcgagatgta cgcctgcaaa ttttggatgg cgggtgttgg gtcttcgatc caactgggct
      481 ggtcaagtgc aaagacagag ttaagacgct gaccgtaata agccagccat ttgtgcaggt
      541 cagccaggaa ctgctgactc gcggcggatt tttcgagggc ggggatgacc tcacccgcgg
      601 cgtgtttgga tagaatctca ctcacctcgg gtatcgaccg ggcagcgcgg ctcaaacgcc
      661 atagggcacg gtcgccttcc atggtcttat tgtcaaagcc ctgggtgagt cgtagagcgt
      721 ccaaggtggt ggcaccttcg aataactcgc aatacaactc ctcgaactgg ctcatggcat
      781 acagcattgg cataatcacg ccatgcaggg cagccatacg ctccacgcgt ttgatcgttt
      841 cggacagatg cgcgcgtaac tgatcgaggc tcatgccgcg caaatcgcag ctttcgaagt
      901 aagcaatgtg ctgtttcatc tccggcagga gttcatcgtt ccaatgggca tcgaatcttt
      961 cgatgatggg attgagggcg gcgtcttgtt gtttcgacat cccagccacc accttgccca
     1021 tcatcagctt gaacacgccc ggcgcggcgc ggttgacaag ccccatggct ttcattacag
     1081 cttccggcgg tagggctttg ggcaccatac ccatatagaa gtatgtgttg atgcgtttca
     1141 ggtgcatttc catatccaaa cccgcctgct caaatccgac accggcgccg accataaaag
     1201 tgccgaccac ggaaaaaatc agtggcggaa caggcgtttt atatgcggga ttcgacatcc
     1261 aggtgatttt cgcctcacgc gggttttccc acttaatggg gaaatcaggc gctgtaggaa
     1321 tgcgttgacc gggctttggt gcttgtgaag ttgaattaga catcataggc tcctttactc
     1381 tcattggtta tgaacagtat aggttgcaaa cctccaccgc acatcggtaa cttacctatt
     1441 tcgcttgcct atttgggttg cctatttcat aaggatatgt cactctgagc ggagcgaaga
     1501 gtctcggctg tggaatgctt gaaggcaata gtagagattc ttcgtcgcta cgctcctcag
     1561 aatgacatga aaaggggcta gtgattggag attagtcctt gctcccctgc cacccggatg
     1621 gcggcatcgc gggattttac acccagcttg tcatagatcg agcgcaagtg tgcatccact
     1681 gtgcggcggc tgacgacgag ccgctcggcg atttgaggat tgctcaagtc ttgcacgagc
     1741 aggcgaagca cctcgatctc gcgcacggtc agggatgtct cagaggcgga tatagcttgg
     1801 gcgacagcat taggctccgg cgggtgagga atcgtccgca ggtcatccag agtcagatgc
     1861 ttgcccgccg cgaactcgcg ggcaaaggct tcgtctccca attgggcgcg taactcggcg
     1921 tgaatatcac caatacccag gaatcgcttg aaggctgctg ccatcccttc gccatacgct
     1981 tggcgggccc cggtcaaagt atctaccaaa cccaaaaccc gtgccgacca cacacccaga
     2041 ccttgccgat tcagaacaac agccagcatt ggcagccaca tgggcaactg atggtcacct
     2101 cggttggcac ctcgctggaa agtgtcttcc aggcggcggc gcgcggtggt cagatcgccc
     2161 tcctgtatat ggcgcaacac acggatgcca gccaggcggc tcaggatacg ctcatcccca
     2221 agttccagcg ccaggcgttc ggcttcgtcc aactgggacg ccatcagggt gtaatcccct
     2281 gtgaataacg cgccactgcc ttgacgagcg agagacaacg ccaacccagc tgggttgttt
     2341 aacgcgcgat aaatgcgtac gctttcagcc atgtaacgag acgcctcggg gggtaattga
     2401 atggagttaa tactaacatc caacatgaca gtagccagct ctgtgtaggc gtacgctttc
     2461 atgacggtat cgggcaaggt ttcaagcagg gaggcagctt cagatagaaa aacctgcttt
     2521 gccgtttgat cctcttgaaa ggcagtgatg cgggtcaatt ccacgagcgc catcagcagc
     2581 ccggtgttat cggcgagtgg gcgatacaac gccaggcttt gttccaacag catccgcgct
     2641 cgagcaaagt cgccctgaaa gcgagacagg gtgcctgccc catacagcgc cttggcacgg
     2701 acagccggtg ccagcgccgc acccaagtca attgcaaggg attcttccag ccaacgacgt
     2761 ccctcgctcc agtaaccacg cgtaagccag aacggttgca acgcgccagc catacgttga
     2821 gccaattcac cctgttgatg ttcgatcgcc cagtgtagtg cggcgcgcaa attatcctgc
     2881 tcacgttcca gccgtttaat ccaagccttt tggtcagcgc ctgtcaggtg cgactcagct
     2941 tcttcaacga acgataaata atagttggca tgcgcacgac gcgcggattc cagttcgtgc
     3001 gtatgtatta gttgctccaa gccaaactcg cggatggttt ccagcatcgt cagacgcggc
     3061 tcagcatcgg tttcgatctg gcgcaggaga ctcttgttca ccaatgaatc gagcgtatcc
     3121 aaagcggttt tctgttcaag cccagcgagc gctgcctgca atgtgcatcc accgacgaaa
     3181 acagccaggc ttcggaatgt gcgttgctcg tcggcattca ataagtcata actccattga
     3241 atggcgttac gcaacgtttg ctggcgggcg ggcaggtcct tcgctccgct cgtgagcaag
     3301 gtgagggatg actcttgcag ctgcgtaagt agggattgtg ggggcagcaa cttgatccgc
     3361 gctgcggcta attcaattgc cagtggcaat ccgtccaggc gggcacaaat ttcagagact
     3421 gcgggtgcgt tcgcttgtgt caatttgaaa tcaggctgaa tcgcttgggc acgttgaacg
     3481 aataacgcga tacccgaaaa ctgcatcagc gtttcgagtg atggctgatc gggtaatgcg
     3541 agcggtgaga gcggaaactc ctgctcaccg cgcaagcgta aagcctcgcg gctggtggcg
     3601 agcatccgca acccggcgca ggcggacaat aactcgctca atagcggcgc agcggacatg
     3661 atctgctcaa aattgtccag caataacaag gcttgcttgc cccgcagaaa ttctttgagg
     3721 ttatccagca gcaaacgctt cggggattct atgacaccca gggcatgggc aatggtcgga
     3781 atgatcaggg tcgggtcgct gatggtggcc agagaaataa agtagacgcc atcagtaaag
     3841 gcatcttgta tatcctgggc cacctgcaat gccaggctcg tcttacccac gccaccggga
     3901 ccggtgatgg tgaccaggcg aacttcggag gagcgcaaga gttggctcag cgtccgaatc
     3961 tcatgctcac gcccgaggag cagggtcatt gataggggga tcgtatttgg cattacgaat
     4021 attatatgtt gattgtttcg ttcttttaga atcgatcgct ttttatttat gacttcaagg
     4081 gattgagagg ggatacaacg tacgttgatt gtatgtccac aggcccggct gattactagc
     4141 tcattgggtc atgttgtaaa caatcaggca agatcgcttc acttgttatg tctatcagcg
     4201 ttccttttct ccttcctgag ggaggatggg cagactcttt gaacttatcg cgaaatcatc
     4261 gcctgtgaat ttggtgaatt cttcgtgggc taattgggca gcgcgaacca tcattttatc
     4321 gaagtggttt agggtgatta gcacgaattt cacggcaggt ctgcgaatga aacgtggcat
     4381 tggtatgtgc atccgcatag cacgccacac gtgtgtacca ccatttggca aggcttcaaa
     4441 atggactgtt tctgtgaaag tatgtttgcc atgctcgtag gaatggatgg tgtagtattc
     4501 aaatggatgc caatccacca ccacttgtgt gctgacggaa ttgccgtggg cgcagtgatt
     4561 gcttgcgccg acacctgagc gtccctgtgg acggtctcct accgaccagg agtcggatac
     4621 accgccccag aggttgcgct tctccgggtt gtggatccat tcccaggtca cagccggagg
     4681 cgtggagaaa tccatttgaa agacgaagtc tgcatcctgc tcggtcagca caatgcagcg
     4741 ggcttcgcaa atttcctgat aacgtgtgtg taaatcaatg ttgaaggtct tgatctcgcc
     4801 gaggctttca tacgcttcca tttggacctg ggcatccgca agctttaaac cattatggtt
     4861 cacgcattgt tcggtgaaca gcatatacgc ccgccagccg gtcgcttcgg tgacgtggtt
     4921 cttggtgagg cggtgaacca ggtttacatc cgagccgacg agttccttga tgttcgcgat
     4981 gctttgttga atatagtcgc cgcaatggat gaagaatttt agatcaagag aaggaatgtg
     5041 acggcaggca ttacaggtac aagtggtggc acgcttaatc gaaagttgtt tgtcacgaaa
     5101 cgccacatac atggattcga caaaatcgat cagtgtgtcg ctgcacgcga atacgtcttc
     5161 agacgcgtat gcaaacacag cgtcaccttc gagtttggag atggtcatga tgggctgaaa
     5221 ccggttgacc agcaacccaa gcagttcaga caggatctcc tgtgaatgtt cgagctctgt
     5281 ctttgccaca aaggatgtgt agccggagat gtctgcgatg accagataac cgtgttgggt
     5341 gggtgttgac atttttagaa taaagctcac aacgcgcggg attccatcag ttctttcacc
     5401 ttgcgtagat cgccgttgtg ctgccgcgtg actgagccag ccagaagagg cataaccagc
     5461 ttgaagaagc cgcgcaactc catctcatat cggatggtta agcgtgtgcc gccctcaacg
     5521 cgctcaactg accttcgaaa tgtcagcggg atgggtccgg atgcggcttt ccacgctgcg
     5581 acccggtttg gctcgtattg gatggtctcg tatatcgttt caatccgctt gccaaaggcc
     5641 ttggcaacca aactaaactc agtacccatt ccaattgggc cttctgaaac tttttttgca
     5701 tcaacccact cagtccgcca ctttagatca ttctcaaggt tggacaatac tgtaaagacc
     5761 tcctcgatgg gtcggttgat cacagtgttc aattcgtagg cttccataaa actcatttcc
     5821 ttttgaatta cctgttggca ggttcgtttg cttccagtaa tcgtttggcg gtgtcaaaat
     5881 tatgcccgtc ccctttctca gcaagtttcc taaacatgcc atcggcaagc ttcaatacgc
     5941 caacgagccc gaactggctt tcgaaggtga tttgcgtacc atgctccttt ggggcaagcg
     6001 taatggtcga ctcgatgctt tcaatgggaa acggtagcct gctgatcgac ttataaccat
     6061 agcggtgggg cggatcccat tgggtgatct cgaacagacc ttcgtttttc cctccagaca
     6121 ttttagataa tactttatac gttgagccgg gaccaggtgc ctcgctggag gtccacgcgg
     6181 ctgacgcgaa atttgaattc cattttgaaa ggttgacagg attactcaag aagtcgaaca
     6241 catcctgttg tgagcggttg ataaaaactc ttaatatgaa tttacccatc gcatgttttt
     6301 cctttgagac agttacctgc ccgccggatc atactgcttc cattaattgt tttagggtat
     6361 tgagttcatt ttggtttatt ttcgaagcca tcttactaat caatcccgcg gcaaacctca
     6421 gtaagccaac cacttcaaaa tcaccaactt gagacactcg tgtaccgcca tcctctggtt
     6481 ccagccgaaa tgaatgagcc atggctttca taggaaacat aacggtgtgt atcttatagc
     6541 cgtaacggtt gggaacatcc cattgagtaa tctcaagacg catttccgtc tctttgcccg
     6601 ccaccttcat tattgccccg agggttgagc ctacaccagg ctcaccgctg gatgtccacg
     6661 ctgctgattc caacattggc atccatgtat gttttattgc aggattgctg aggaaatcga
     6721 atacatcctg ttgtgaacga ttgacaaaaa cgctggctgt gaacttttgc atttcaaact
     6781 ctcctttgca taccacaatc aaactctggg gttttactta ttctcaataa tgttgactga
     6841 agtgcaattt ccagaaggtg acatcgggaa gatgatcgtt gcgcaacatt agctgccacg
     6901 atcattttcc caaagataga ggctcaattt gccgaaatct tgaaatccca gacggcggta
     6961 tacgttgtac ccatttaggg aggcttgcag gacaccgatc tgataaccca gctcacgtgc
     7021 ccttaacagc ggagccaatg tgatggcgga gccgattccc tgaccacgcg cttcgggcag
     7081 gcaggtaatg ttatagattc ctgccacgcc ttcagacagg aataactgtg atgtgccaac
     7141 cggttttcca tctaataggg cgagataggt ttgaaatcgc ggctcaaaaa tagcatccac
     7201 aaaaaaatcg taccaaacct gctcaaactt ctcgtcgatt ctgaagccaa cacttgcaat
     7261 gtgaatccat tgcctcagcg tctgttcatc gtcgacccgc acgattctca agccgggatt
     7321 agtcggcagg caacctggca atagtgacag atcgacggcc atctcagcgg caaacgaatc
     7381 gctgaatttc aaaccatgcg caagcaaaac ctgattgatg ttgacagata gtgggtctgc
     7441 tcgcgtcagc caggagagtc gcctgatgtt catcgacttg aaacgaatca gtgtgctctc
     7501 gatcagatcg tcaagacctt caagtggcaa ttgatggcat accaccaggt tcatgaaatg
     7561 atccggcagg tctgtcatca gccaggtcag ataacgacca acggacaatt ccacgtttgg
     7621 tgactgcgcc aggcaataat gataggcctc ccagtttgcc ttgatggcgg ctttcacaga
     7681 tttcctttca gcgagttcac tcaagtcatt tgtcatgtta agagttgatc gagataattc
     7741 tggggcaaag ccggccaaca tttgaagaga ggatctgttc atagccgaag attatctttc
     7801 tgttttgcca tctgatcgcc agcgaagacg atcaggtagg tgcagatacg tttgatgaac
     7861 aaggttctta aaacggtttg aatctcatca gccagagcag gatggcaagc ccaacgatac
     7921 ctacaaccga cacgatcagc gcattcggcg cgtgacgcgc cttatcaaat tctttttgcg
     7981 cggcgggcaa gtccacatct gtttttctgg cttccatcag gcgctgggca gcgttaaaga
     8041 tcatgccgta gtaagccgcc ccatattgct gcatgaccca ggctacgatc acgaagacaa
     8101 gcacggaaag ccaaacccac cattgtcgcc accacacagt cataaatgca ggagcaaagc
     8161 cgaagagccc catggcaagg gtgagatacc tgacgtactt gatgtcgggt acattattga
     8221 agaatgtcag gcttcgttca gggtcgggct cgtcgcggaa tttcaacatc gcggcggcat
     8281 gggctccgtg tgagagcatg aagagaaagg cgaagaagat gtgcaaaaat accagccagt
     8341 tgtacatatc agactccgta gagaccaaac ctatgctctg tcattgcgaa gcgatctgcc
     8401 tgcctgctgc gacggaccca aactagatac tgcgcatatt tcgccgcaca ataatgccca
     8461 aaagcaaacc agaaaccagg atatgaacga tgagagccac gattaactcg ggtattgttg
     8521 gagttggtaa tggaggtgtc gcactgagcg gcgttacgat catgttcatg acaatgtaaa
     8581 cgccaagtcc gtacagaagc gagccaggga taatatagcg ccgcagaagc ggaatgcggt
     8641 cgactcccag gatgaaaata ccggcgatga taatgctgaa gataaagtgg aataacactc
     8701 caagcagggc ggtggggatg ccgcctgcaa aggcggattc acccaatgcg ccactggcta
     8761 tatattgata gagagtaatc aatgggaccc caccaaggac attctgaaca aaccagtggt
     8821 agatgatcac atcggctgta ccgaggatca gtccggcgaa cacacttagg cggaggagtg
     8881 aattgcggga aatgggagat gaactggtga tagtagacat gttattagtt ccttttctgt
     8941 aattatgaga gttaatggtt gctgccgatc agctttcttt tttatttatt catatttcga
     9001 cgcacaagaa ttcccaagag caacccgtag aacagcaggt gatccaggat gatcaagagt
     9061 agctctggcg ttgtcggagc tggtactgga ggtgtcgcgc tgagcggcgt tacgatcaag
     9121 ttcatgacaa tgaaaacgcc aaatccatac aggagcgagc cagggataat atagcgccgc
     9181 agaagcggga tgcggtcgac tccgaggatg aaaacgccag cgactactat gcttatgagg
     9241 aagtagaata gcactccgat aaaagctgtg gtgacgccac ccgcgaaggc tgattcaccc
     9301 aatgcgccac tggctatata ttgaaaaaat gtaattaatg ggatcccaaa caggacattt
     9361 acaacaagcc agttataaat gatcatgtcg gctgtaccga taatcaatcc cgtgaccaca
     9421 ctaaagcgga gcagtgaatt gcgggaggcg ggaaatgtgg tggcgggagt agacatgata
     9481 aagattccat tctttggata actaatcgtt cgtcgctaaa acatcgtgtt gtcgtttgct
     9541 gacgtttcag gaatttcctg agcgacatcc cagtgttcaa cgatcttgcc attttcaagc
     9601 cgaaagatct ccacaaccgc gctgccgcgg tcaccgggct tgaggatcaa gtggctatgt
     9661 atagctacca aatcgccttc agcaatgatc ctttttgttt ccatgcgtaa ttctggaaag
     9721 ttttgcatga tccatttcat ggtattaata aatgcttctg gcccatctgc tgagccggga
     9781 ttatgctgtc gatagttcgg cccaagatgt ttggcgacca tttcttcggg cttctgtagt
     9841 gcaagctcat gccattcgcg tacgattctc ttgttttctt ccaattgttc gttcatggtt
     9901 cctccaaaaa ttcttaattt accgactatg gtgaatggct taagtcaact gcttgaggac
     9961 tgtcgctctg aatggtgcag ctaacaagtc ttcaagcggt gcatacgcac cagcattcat
    10021 ggcttgttgc atggcagttg cccgtgcctc ggcggatgcc caatcggcga tctcaaccag
    10081 gatgtcaggg ttgtcaatca cttcatattg caggctgccc agaaaaccag gttgcactga
    10141 tccgtatttc gccactacgc ttttgagcaa atttttaagc tctgcccgtt tgcctgattt
    10201 ggcatgcagt tccacgatca ctttgattgg cgcttcattt tcagttggca tggtttgctc
    10261 ctaaggtagg ccagatttga ttactctgtg tattgaagta gtttcggtag tgattgcaga
    10321 tacagccaga tggcctgcaa ctcataatca tggaggttac ggtattcgtt ccacggcatg
    10381 agcttggggt ccaattcatg gccacttggc gttttacctg tgcggatcgt attgatgaaa
    10441 tcagcttccg tccaaaaggc aagttcgccg cccggtgtca gatttgggga gatccgcgtg
    10501 atggtcgggt cggggaacgg accgccattc agttccggtc catgacagat gcggcaatcg
    10561 ttgatattca ccgcatattc gccatatgcg gcacttgctc cccgttcggg tgcgctgaca
    10621 tgcacttcgt gggtcacctc ctccaccggc ata
//
LOCUS       DASTJP010000718         4484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_590358, whole genome shotgun sequence.
ACCESSION   DASTJP010000718 DASTJP010000000
VERSION     DASTJP010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590358"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFQ23_20995"
     CDS             complement(<1..977)
                     /locus_tag="VFQ23_20995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9909139.1"
                     /translation="MTARKVRSLLIHILIVGAILTACAPASTEAPAATEPAQPAAGSE
                     LNVLCTPQEQWCQGMKQEFEAKYGITVNYVRMSSGEALARVQAEKDNPQFDIWWGGPI
                     DSFVAAKGEGLLETYESPNFGNLTDPAKMKDVDNQWVGVYVGTLGFATNTDWLAANPD
                     AAAPTSWDDLLKPEFTGQVMVAHPSSSGTSYTALATVLQIRGEEAGWEYLDKYASQVS
                     QFTKSGAAPAKFVGQGEAAVAIVFSHDIVNEIENNKLPLVLTFPEEGTGYEIGGMAIL
                     KGAKNLDAAKLWYDWALTPEAQALGPTYAAYQAPTVSGVELSHPELLEV"
     gene            1267..2319
                     /locus_tag="VFQ23_21000"
     CDS             1267..2319
                     /locus_tag="VFQ23_21000"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9909140.1"
                     /translation="MKNSRSITIKELALRAGVSHSTVSRALNNHPSLARGTVERIQKL
                     ARELNYVPSGFARSLKTNRSYMLGVIVHRIADPFYSEVLAGIQNVAHQFQYSVFVSAF
                     ESDLERQDSLIRGVLGRQADALIIGDSVFTAAHVDKLGIRVPTVFIHNRAEILPNSIY
                     HDDLNATRALTRHLLDLGHSRIAYGGNQRAGLLNQQRREGVVTELQSAGLELQSPYDL
                     MAPDGQMSSGAWLAKQLLNMTERPTAIICFNDMQAIGVMQSLQQANLRIPEDISVTGF
                     DNIPLAEFLYPSLTTYSQPIWELGQTAAKVALKLLGETLDEPLVFESLITMHGHLVVR
                     RSTAAPAAEAQVEPAT"
     gene            2482..3435
                     /locus_tag="VFQ23_21005"
     CDS             2482..3435
                     /locus_tag="VFQ23_21005"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET9909141.1"
                     /translation="MFVKKLNLLIVSITILALVGACAPAQATEPAAGGDGNITIGVTM
                     KFDDLWLTTLRDAMTEYAATQPGVELVMVDSKEDVATQLGQVENFVAQGVDAIVVIAA
                     NTDAADPMTKAAQDAGIPLLYVNRLPSNLPEGISYVGSESIQAGIMQAEWIAEQLGGK
                     GNVVIMNGDLAQEAAQKRTEGEKQVFAKYPDITIIREDTGNWSRDQGLALMENWLASG
                     DQIDAVAANNDEMAIGALQAIEAAGQLGQIIVGGVDASPDALQEMDKGRLDVTVFQNA
                     KGQGEGGIKVAIALARGESVEKYTWIPFELVTPENYKEYMN"
     gene            3507..>4484
                     /locus_tag="VFQ23_21010"
     CDS             3507..>4484
                     /locus_tag="VFQ23_21010"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET9909142.1"
                     /translation="MSENDILRMENISKSFPGVKALSDVNLTVRMGTIHALMGENGAG
                     KSTLMKILDGIYTPDSGQITFQGQPVTIDNTHTALKLGISMIHQELSPIPHMTVAENI
                     FLGREPLGRFGLIDKRKLNGDTKALLQRLEIDIDPESTMKDLSVANTQMVEIAKAISY
                     DSSLIIMDEPTSAITEREVAHLFRMIRSLKEKGVTIIYITHKMDEVFQIADDITVLRD
                     GRHVSTVTATQTNKYNLITMMVGRELTELFPKEVAPIGETVLSVRNLTRAGIVEDVSF
                     DLRRGEILGIAGLMGAGRTEVIEAIFGIHKLDSGEIIIKDKKTQINTPAD"
BASE COUNT         1156 a         1064 c         1185 g         1079 t
ORIGIN      
        1 ttgacttcga gcaattcggg atgtgaaagc tcgacaccgc tgactgtcgg tgcctgatag
       61 gcagcatacg tggggcccaa ggcctgtgct tcgggtgtca gcgcccagtc ataccagagt
      121 tttgccgcat ccaggttttt agcacccttg agaatcgcca taccaccaat ttcatatcct
      181 gttccttctt ccgggaaggt cagcaccagg ggtaacttat tgttctcgat ctcatttacg
      241 atatcgtgag agaaaacaat ggccaccgcg gcttctccct gaccgacaaa cttggctggc
      301 gccgcgccgc tcttggtgaa ctgggaaacc tgcgatgcat atttatccaa atactcccac
      361 ccggcttcct cgccgcggat ctgcaggacg gtcgcaaggg ctgtgtagga tgttcctgaa
      421 gaagacggat gagccaccat tacttgcccg gtgaactcag gtttgagcag atcatcccac
      481 gaggtcgggg cagcggcatc ggggttcgca gccagccagt cggtgtttgt ggcaaagcca
      541 agtgtgccca cataaacacc aacccactgg ttatccacat ccttcatctt tgctggatct
      601 gtgaggtttc caaaatttgg tgactcatat gtctcaagca atccttctcc ttttgcagcg
      661 acaaagctat cgatcggtcc accccaccag atatcgaatt gcgggttgtc cttctcagcc
      721 tggacgcgag ccaaggcttc acctgaggac atgcgaacgt agttgacggt gataccatac
      781 ttcgcctcaa actcttgctt catgccctga caccactgtt cctgcggggt acatagcaca
      841 ttgagttccg agccagcagc aggctgcgct ggctcggttg cagcaggggc ttcggttgaa
      901 gccggcgcgc aagccgtgag gatcgcgccc acgatcaaaa tgtggatcaa caaactgcgt
      961 acttttcttg cggtcatctt cttctcctta tatatagaat agggtgggca tttgaaaata
     1021 cccacattcc aagcgggaat caatttcccc cgagcgttga ccatagtgaa ctataaggaa
     1081 caaaaaccat agtgtcaaga ggtaatcttc aatcataatg ggagcagttt tttgatttgg
     1141 ggatgaagcg cgctatatca gcaaagttaa cctacttttc ccttgacagg tgaattctta
     1201 gtgctatact gctgcaatcg tgtgcagcac acgtgtgcat tccctcaaaa aacatattga
     1261 tataagttga aaaactccag aagtattacg attaaggagc tagctcttcg agcgggtgtc
     1321 tcgcattcaa cggtatcgcg ggcgctgaat aatcatccat ctctggcacg cggaaccgtg
     1381 gaacggattc aaaaactggc gcgggaatta aattacgtgc ccagtggttt tgcgcgtagt
     1441 ttaaagacga atcgttccta tatgttgggg gtaatcgtcc atcgtattgc tgatcccttc
     1501 tatagcgaag tcctggccgg aatccagaac gtggcacatc aattccaata cagcgtgttt
     1561 gtatcggcat ttgagagtga tttggagcgc caggatagcc tgatccgagg cgtgctgggg
     1621 agacaagcgg atgccttgat catcggcgat tcggttttta cagcggcgca tgtcgacaaa
     1681 ctggggatac gggtgccgac tgttttcatc cataaccgcg cggaaatttt gccgaactct
     1741 atttatcacg atgatttgaa tgccacacgc gcgttgacgc ggcatttgct ggatttggga
     1801 cacagtcgta tcgcctacgg cggcaatcaa agagcggggc tcctcaatca acagcggcga
     1861 gaaggagtcg ttactgaact ccaaagcgcc gggctcgaat tgcagagtcc ctatgacttg
     1921 atggcgccag acggacaaat gagcagcggg gcgtggctgg caaaacaact gttaaatatg
     1981 acggagcgcc ccacggcgat tatatgcttc aatgacatgc aggcgatcgg agtaatgcag
     2041 tctcttcaac aagcgaattt gcgtatcccc gaagacatct ctgtaactgg tttcgacaat
     2101 attccgctgg cagaatttct ttatccttct ttaactacat attcacaacc gatttgggag
     2161 ttaggtcaaa cggctgctaa ggttgccttg aaactactcg gtgaaaccct ggatgagccg
     2221 ctggtgtttg aatccttgat tacgatgcac ggtcatctcg ttgtgcgccg atctactgcc
     2281 gcgccagctg cggaggcaca agtagaaccg gcaacctgat cctactgtta tttttattaa
     2341 cttgaatcga aacatgaatt cgttgcgaag ttcgcccaac aatgaggttc ataaaaggag
     2401 gtgaaacggc gagaaacatg attgagtgaa acgtgttcat tgactcgaac tacatcaaga
     2461 aaggataaag gagaaaatcc aatgttcgtc aaaaaattga atttgctgat cgtgagcatt
     2521 acaattctgg cactcgtagg ggcttgtgct cccgcacagg caaccgaacc cgccgccggc
     2581 ggagatggaa acatcacgat cggcgtgacg atgaagtttg acgatctctg gctgacaacg
     2641 ttaagggatg cgatgacgga gtatgctgca actcaacccg gtgttgaact ggtcatggtc
     2701 gattccaagg aagatgtggc aactcagctg ggtcaggtgg aaaactttgt ggcgcagggc
     2761 gtggatgcca tcgtagtcat cgcagccaac acagatgctg ccgatccgat gacgaaagct
     2821 gcccaggatg caggcattcc tcttttatat gtcaaccggc taccttcgaa cttgcctgaa
     2881 ggaatatcgt atgtaggctc tgagtccatt caagctggaa tcatgcaggc tgaatggatc
     2941 gctgagcaac tcggcggcaa gggaaatgtc gtcatcatga atggagattt ggctcaggaa
     3001 gctgcccaga aacgcaccga aggcgaaaaa caagtctttg ccaaatatcc tgatattacg
     3061 atcatccggg aggacactgg caactggagc cgtgaccaag gcctggccct catggaaaac
     3121 tggcttgcct ccggcgacca aatcgacgct gtcgctgcca ataatgacga aatggcgatc
     3181 ggtgccctcc aggcgattga ggccgcgggt caactgggcc agatcattgt tggtggcgta
     3241 gatgcctctc ccgatgcgct gcaggagatg gacaaaggca ggctggatgt cacagtcttc
     3301 cagaatgcga aaggacaggg agaaggtggc attaaggtcg ccatcgctct ggcccgtgga
     3361 gaatcggttg aaaagtacac ctggattccc ttcgaactgg tgacgcctga gaattacaaa
     3421 gagtatatga attagtattt cgtaagatgg cgggatgact gcgaagcgtc ccgccatttt
     3481 gtcacgtcaa atgtggagca actcgcatga gtgagaatga tattctccgg atggaaaaca
     3541 tctcaaaatc gttcccgggt gtaaaggcgt taagtgatgt aaatttgacg gtacgtatgg
     3601 ggacgatcca tgcgctgatg ggagaaaatg gcgccggaaa gtccaccttg atgaagatac
     3661 ttgacggtat ctacactcca gatagcggtc aaattacttt tcagggacag cctgtcacga
     3721 ttgacaatac tcatacagcc ctcaaactgg gcatttcgat gattcatcag gagctgtcgc
     3781 cgatcccaca tatgacggtg gcagagaata ttttcctcgg gcgcgaaccg ctgggtcgtt
     3841 ttgggttgat tgacaaacgc aaattaaatg gcgataccaa agctttgctt cagcgactcg
     3901 aaattgatat tgatccagag agcacgatga aagacctaag cgttgcgaat acgcagatgg
     3961 tcgaaatcgc caaggccatt tcctacgact ccagtttgat catcatggat gaacctacct
     4021 cagccatcac cgaacgcgag gtagcacatc ttttcagaat gattcgctct cttaaggaaa
     4081 agggcgtcac aattatctat atcactcaca agatggatga ggtctttcag attgctgatg
     4141 atattacggt tttgagagat ggcaggcatg tttccacggt aacagccacg caaacgaaca
     4201 aatacaattt gatcacgatg atggtaggac gcgaattgac cgagttgttt ccgaaagagg
     4261 tagcgcccat cggagaaact gttttgtctg ttcgcaacct gacgcgcgcg ggtattgtag
     4321 aagatgtcag ttttgacttg cgtcggggcg agattttagg gattgccgga ttgatggggg
     4381 cgggacgaac ggaggtgatt gaggcgatct tcggtattca taaattggat tcaggggaga
     4441 tcattattaa ggacaaaaag actcaaatta acacacctgc tgat
//
LOCUS       DASTJP010000719         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1013706, whole genome shotgun sequence.
ACCESSION   DASTJP010000719 DASTJP010000000
VERSION     DASTJP010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013706"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFQ23_21015"
     CDS             <1..196
                     /locus_tag="VFQ23_21015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909143.1"
                     /translation="EQRNGGTRAFDELANEYKNRPDKAANILAPTELQCDWLREIGYE
                     DVDCYFRIYELAVFGGIKPK"
     gene            305..889
                     /gene="leuD"
                     /locus_tag="VFQ23_21020"
     CDS             305..889
                     /gene="leuD"
                     /locus_tag="VFQ23_21020"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET9909144.1"
                     /translation="MAQFTTLTSRVVQLPVNDVDTDQIIPARFLKATDKNGMGDHLFA
                     DWRYNSDGSPKADFVLNKPESKGAQILLAGDNFGCGSSREHAPWALTAFGFRAVISTS
                     FADIFRNNSLKNGLIPIIVDDATHKMLFDLIEEVPNTELAVDLESQTLSFPHGSVTFP
                     IDAFNKTCLLNGVDELGYIMGFEKEIAAFESQLT"
     gene            963..2519
                     /gene="cimA"
                     /locus_tag="VFQ23_21025"
     CDS             963..2519
                     /gene="cimA"
                     /locus_tag="VFQ23_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET9909145.1"
                     /translation="MQQIQIYDTTLRDGTQSEGFTLSSNDKIRIAHKLDELGVAFIEG
                     GWPGSNPKDVEFFERVRDMQWNNALIAAFGSTCRVKGGPEDDANIKALLDSHTPVCTI
                     FGKTWSLHVKEVLLTTYEDNLRIIEQSVAYLKANGKRVIYDAEHFFDGYKADSSYAIE
                     TLQAAIRGGAEIVVLCDTNGGTLPWELEQIIRDLKPALNHPFGIHTHNDSECAVVNSL
                     TAVREGAIQVQGTINGVGERCGNANLISIMADLELKMGYQCLPPGQINCLYELSHFVA
                     EVANITPDEHLPFVGKSAFAHKGGVHVAAMRRSAQSYQHVEPELVGNKMRVVVSDLSG
                     RGNLLSKAEEHGVEVEGEEVLPVLNDIKELEARGFSFEAAEASVAMMLKRQEYGYKPP
                     FELVDFSVNVEHRQGRGIFAEATVKVRVQGELMHTAAEGNGPVNALDNALRKALVSYY
                     PQLAKFHLSDYKVRILDSDHGTEAITRVLIDTRNSTTRWSTVGASTNIIEASWRALAD
                     SMEYGLMVAH"
     gene            2574..>3653
                     /gene="leuB"
                     /locus_tag="VFQ23_21030"
     CDS             2574..>3653
                     /gene="leuB"
                     /locus_tag="VFQ23_21030"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET9909146.1"
                     /translation="MNFNITLLPGDGIGPEVVNEGARVLDVVATKFGHTFSFQERLMG
                     GCSIDKYGSSLTDETLADCQSADAVLLGAVGGPKWDDPNAKDRPERGLLALRKGLRVF
                     ANLRPVKVHPALVDSSPLKPEKLQGVDILVVRELTGGLYFGQPKMREIKDGKERAVDT
                     LEYYDFEICRVLELAFKLARDRKKKVTSVDKANVLESSRLWRQLATQVGKDNPDVELE
                     HVLVDTASMRLITGPAWMDVVVTENMFGDILTDEASVLAGSMGMLPSASLSEAGVGLS
                     SERRMGLYEPIHGSAPDIAGKGIANPIGTILSCALLLRYSLKLEAEARSIEKAVDETI
                     TAGARTTDLGGTLTTRQMTDEIINKI"
BASE COUNT          918 a          882 c          951 g          902 t
ORIGIN      
        1 cgagcaacgg aacggtggca cgcgtgcctt tgacgagctg gcgaatgaat acaaaaatcg
       61 cccggataaa gctgccaaca tattggcgcc tacagaactt caatgcgact ggctgcgaga
      121 gatcggctac gaagacgtcg attgctactt ccggatctat gaacttgcag tgtttggagg
      181 aataaaacca aaataaatgg tttgtcattg cgaggcgata gcccgaagca ggctcatctt
      241 taaacaggag attgcttcgt cgctcgctgg cgcttgctcc tcgcaaagac attaaggaat
      301 aattatggct caatttacta ctcttacctc tcgtgttgta caacttccag tgaacgatgt
      361 ggacactgac cagatcatcc ctgcccgctt cttaaaggcc accgataaaa atggcatggg
      421 cgatcacctc tttgcagact ggcgctacaa ctcggatggc tcgccaaaag ccgattttgt
      481 cttgaacaag cctgaatcaa aaggcgccca gatcctattg gcaggtgaca acttcgggtg
      541 tggttcaagt cgcgagcacg ctccctgggc tctcactgcg tttggatttc gtgccgttat
      601 cagcacctct tttgccgata tcttccgcaa caattcactc aagaacggac tcatcccgat
      661 cattgtggat gatgcaaccc acaaaatgtt gttcgacctg attgaagaag ttccgaacac
      721 ggaactcgct gttgatcttg aatcacagac tctttccttc ccgcatggtt cggttacatt
      781 tccgatagac gcgttcaaca aaacctgttt gcttaatggt gtggatgagc tcgggtacat
      841 tatgggtttt gaaaaagaaa tcgcggcctt cgagtcacaa cttacttagc cacagcgatg
      901 cggagccgca gagaaaaata attctccgcg actctgtgcc tctgtggcaa aacggataaa
      961 ctatgcaaca aatccaaatt tatgatacga ccctccgcga tgggactcag tctgaaggct
     1021 tcacgctttc gagcaacgac aagattcgca tcgcgcacaa gctggatgaa ctcggtgttg
     1081 cttttatcga aggcggttgg cccggttcaa accccaaaga tgttgaattc ttcgagcgtg
     1141 tccgcgatat gcaatggaat aacgcgctca ttgcagcctt cggctctacg tgccgtgtca
     1201 agggcggacc ggaagatgac gccaatatca aagccttgct tgattcacat acccccgttt
     1261 gcacgatctt cggtaagacg tggtctttgc atgtcaagga agttcttctc actacctacg
     1321 aagataatct tcggatcatt gaacagtcgg tagcctatct caaagccaat ggcaagcgtg
     1381 tgatctacga tgctgaacat ttcttcgatg gctacaaagc cgattcttca tacgcaatcg
     1441 aaacgctgca ggctgcgatt cgcggcggcg cagaaattgt cgttttgtgc gataccaatg
     1501 ggggaacatt gccatgggaa ttggagcaaa tcattcggga tctcaagcct gcgctcaatc
     1561 atccgtttgg aattcacact cataacgact ccgagtgtgc tgtcgtcaac tcattgaccg
     1621 ctgtacgcga aggcgcgatc caggttcagg ggacgatcaa cggagtcggt gaacgttgtg
     1681 ggaatgcgaa tctgatctcg atcatggctg accttgaact caaaatgggc tatcaatgtc
     1741 ttccaccagg gcagatcaac tgcctttatg aactctcaca ctttgttgct gaagtcgcca
     1801 atattacacc tgacgaacat ttgcctttcg ttggcaaatc cgcctttgct cataaaggcg
     1861 gtgtccatgt tgcagcgatg agacgttcag cgcaatccta tcagcatgtg gaacctgaac
     1921 tcgtcggcaa caagatgcgt gtggttgttt cggatctttc tggcaggggc aatttgttga
     1981 gcaaagccga ggaacatggc gtggaagtgg aaggggagga agtcctgccg gtcctcaatg
     2041 acatcaagga attggaagcg cgcgggtttt ctttcgaagc tgccgaagcc tcagtggcaa
     2101 tgatgctcaa gcgacaggaa tatggttata aaccgccttt tgaacttgtt gatttttcag
     2161 ttaatgttga gcatcgccag ggacgcggca tctttgcgga ggcaacggtc aaagtgcgcg
     2221 tgcagggcga gctgatgcat accgctgcag aggggaacgg cccggtcaat gccttggata
     2281 atgcattaag aaaggctttg gtttcgtact atcctcagct tgcaaaattt catctctctg
     2341 attacaaagt tcggattctg gactcggatc acggcacgga ggcgattaca cgtgtgttga
     2401 ttgacacacg aaatagcacg acccgttgga gcactgttgg cgcgagcacc aatatcatcg
     2461 aagcctcgtg gcgcgcgctg gcagattcga tggaatatgg cttgatggtg gcacattaat
     2521 cttaatgcgt agtaagggcg aagcatgctt cgcccttaca gaatggaaat aatttgaatt
     2581 tcaacatcac tctcctccct ggcgatggta tcggccccga agtagtgaat gagggtgcac
     2641 gcgtcctgga cgtcgtcgcc acaaaattcg gccacacctt cagttttcag gaacgcttga
     2701 tgggcggttg ttccattgac aaatatggtt catcgctcac agatgaaact ctggccgatt
     2761 gccaatcggc ggatgccgtt ctgcttggtg cagtaggcgg accgaaatgg gacgacccaa
     2821 atgccaaaga ccggcctgag cgcggcctgc ttgcccttcg taaaggactg cgcgtatttg
     2881 ccaatctgcg acccgtcaaa gtgcaccccg ccttagtaga ttcgtctcca ctcaagccgg
     2941 agaaacttca aggtgtggat attcttgttg tgcgtgagct aacgggtggc ttgtattttg
     3001 gtcagcccaa aatgcgcgaa ataaaagatg gcaaagaacg tgcggttgat actcttgagt
     3061 attatgattt tgagatctgc cgggtgttgg aattagcttt caagctcgcc cgcgaccgga
     3121 aaaagaaagt aacatctgta gacaaagcca atgtgttgga atcctcgaga ttatggcgac
     3181 agctcgcgac tcaagtcggg aaagacaacc cggatgtgga attggagcat gtccttgttg
     3241 acacagcgtc gatgagattg atcacaggtc cggcctggat ggatgtcgtt gttacagaga
     3301 atatgtttgg cgacatactc accgatgagg catctgtcct ggcgggttcg atgggaatgt
     3361 tgccctcggc aagtttatcg gaggcaggcg tcgggttgtc ctctgaaagg cgcatgggac
     3421 tttatgaacc gatccacgga tctgctcctg acattgccgg caaaggcatc gcaaatccaa
     3481 ttggtacaat cctttcctgt gcattgctgt tgcgttattc actaaaattg gaagctgagg
     3541 cacgatcgat cgaaaaggct gtagatgaaa ctatcaccgc gggcgcacgc acgaccgacc
     3601 tcggtggcac gttaacaacc cgtcaaatga cggatgaaat tattaataaa ata
//
LOCUS       DASTJP010000720         7300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_682897, whole genome shotgun sequence.
ACCESSION   DASTJP010000720 DASTJP010000000
VERSION     DASTJP010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682897"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFQ23_21035"
     CDS             <1..633
                     /locus_tag="VFQ23_21035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9909147.1"
                     /translation="TMIAMIASGGLVTWILLTDGLRDISFQFSENLFPVYMQEFGGLS
                     LQQIGWVTSVFGICMMLTTIPGGWLSDKVGERVGIAAGMILMSGALFLLINIPAGIQW
                     LYYVGWGLAGVGIGILTPAYQSLISKAVPAHMRGTAFGLFSTSLGVISLPSPWIGAQL
                     WDRVNPQFPFMVTAIVLLLSVIPIWIKFKLPKTVENVRVPSEEQAAVTTA"
     gene            658..1035
                     /locus_tag="VFQ23_21040"
     CDS             658..1035
                     /locus_tag="VFQ23_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909148.1"
                     /translation="MASVEERMKILKMIEEGKLSAEEGTKLLAALSERRGPPARMPGM
                     PGPARWLRIRVTDTRSGRSKASVQIPLALVDAGLKIGAHFAPQVEGVDMSNVMEALRS
                     GVTGKIIDVTDDEDGEHVEIYVE"
     gene            1078..2424
                     /locus_tag="VFQ23_21045"
     CDS             1078..2424
                     /locus_tag="VFQ23_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9909149.1"
                     /translation="METNTNRPRGMFGFTVVWLGQIVSVLASSMSQFALTIFMYEETG
                     SATALGLMQVFFITPFLIISPIAGVMIDRYNRKMMMMVSDLAAGIATLAILAFQALGL
                     LEYWHLYAASIIYGLGMAFQWPAYSAAISTMVPKEQLGRANGMMSLIEAGPGVIAPIL
                     AGAVLPLIGLTGILVFDVVTFLVAIGALLVVHIPQPPRTTEGSQAKGGMLQEAAYGFK
                     YIFARPSLLGLQMIFFAGNLFAGIAFTLLAPMILSRTGNDSLMLGSAQTAGAVGGLVG
                     GVLMSAWGGFKRRAHGVLFGWIWSGLTMAIVGFAGGLPMWIVGLALTALAGPLVNASN
                     QAIWQSKVAPDLQGRVFSARRLIAWFTNPISPIIAGTLADYVLEPAARAETGLPATFA
                     WLVGTGPGSGMGLLIVFCGVASAVVGLLGYFIPAIYNAETILPDYDALGPAKPEPA"
     gene            2510..4006
                     /locus_tag="VFQ23_21050"
     CDS             2510..4006
                     /locus_tag="VFQ23_21050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9909150.1"
                     /translation="MTGLDFLIRRPNGMRGFYVIWSGQMISGIASNITFFALPIWILN
                     MTDISGGALGLWESFYFGSYLVVVLVAGVFIDRYSRKRMMLVYDFLSLSATAILLVLQ
                     STDNMEVWHLYIAAIFQGIGFAFHSPSYSAAITTLVPRRQYVRANGLMSLLYDAPEIF
                     GPVLAGVLYLTIGLGGILALNLLAFVVSIGALLFVEIPPTPHTREGQMNQESFWSEAI
                     YGIKYILRRPGLLGIQLIFFFGNFFSGIALSVTALFTMILLRSGDASAAGTVQSLGAL
                     AAVVAGVYLSTLGGIKRPVRAILLGWVISSLFGLTLLGVGQVFIVWAIAMVLNTIFEP
                     VVNVAVETFMQTKIPPDLQGRVFSASDFLAQAMVPITPLLAGFFGEGIFEPAMAEGGP
                     LANIFGWLVGVGPGSGFGLMILLCGIGGTLIGLSGYLIPAIRNVDDYLPDYTPLPPAG
                     VVLRIRPRTDKRKARDSANKRRIKKTVNKSTREAGEERINIEPEEKRD"
     gene            complement(4048..4848)
                     /gene="arsM"
                     /locus_tag="VFQ23_21055"
     CDS             complement(4048..4848)
                     /gene="arsM"
                     /locus_tag="VFQ23_21055"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HET9909151.1"
                     /translation="MTTNTPIHEAVREHYAERISNNASCCGPSSDCCGPDSNLYPSEL
                     LSILPEGESAISYGCGDPITLAALQPGQTVLDLGSGAGLDCFFAAKKVGETGKVIGVD
                     MTPQMIERARASAQRLNLNNVEFRQGYLEDLPVESDSFDVIISNCVINLSPDKSKVFS
                     EAFRVLNSGGKLAVSDIVTDGPLPASIKQSLSAWAGCVSGAVDAKDYVGMMEAVGFKN
                     ISIKPVFFDQATVDSAINDLGDTIELKTLSRDDVYKSVYSAKITAYKP"
     gene            complement(4878..5843)
                     /locus_tag="VFQ23_21060"
     CDS             complement(4878..5843)
                     /locus_tag="VFQ23_21060"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET9909152.1"
                     /translation="MDIITLLQYATNKLFETFANNWVLLLLSILISAAIKVYLDEDAI
                     ARFLRRNTKNSVIMSTGVAVATPLCSCGTTAIVLGMMASTIPWAPIVAFLVASPLTSP
                     QELFYSAGLFGWPFAIAFFFASILLGLTGGAIASFAEGRGWLANQVRMAPPKASSLSL
                     GIMGGPAPIGLPVLPPALAEPIKTPASLRLFTQETFNVSKSLLPLFVGFTFIGYLLNG
                     LIPSAWISSLFGTGHAYSIPLAATLGLPFYINTEASLPLVRAMLESGMSEGAALAFLI
                     TGAGTSLGALGGALTIARWRVVGIVIGTLWLGSILLGFAYNLFVI"
     gene            complement(6116..6433)
                     /locus_tag="VFQ23_21065"
     CDS             complement(6116..6433)
                     /locus_tag="VFQ23_21065"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9909153.1"
                     /translation="MKTPLNPVQFAKAIADETRQKIMNECCCCTLSVSEIVEKVGVSQ
                     PTVSHHLAILRDAGLVDIREEGKQTFYTLNQECVAVCCGQLMIKFAPEEKATELVLKV
                     VST"
     gene            complement(6430..6765)
                     /locus_tag="VFQ23_21070"
     CDS             complement(6430..6765)
                     /locus_tag="VFQ23_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909154.1"
                     /translation="MNSVPRDTIIFCNMDVFTPMERESHIQTTRRLFQAVQSIHETQN
                     GYEFLFPNDGSEGILDFARFISEERRCCSFLEFTLKIPSTNDPISLILTGPEGTQEFL
                     REEFSEAFS"
     gene            6884..>7300
                     /locus_tag="VFQ23_21075"
     CDS             6884..>7300
                     /locus_tag="VFQ23_21075"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9909155.1"
                     /translation="MAANAVRALILSGGGGRGAFHAGVYKYLMRQHKLNLDADHDDVW
                     DPQIIVGTSIGAVNGAAIAQGMSADRLVEVWKSLEEKDIQGLPPGMRGIARWVAGTIF
                     KEVMDTKLPQIHPKEATSPIPKEFWPPLPLLPNWIAE"
BASE COUNT         1724 a         1767 c         1896 g         1913 t
ORIGIN      
        1 accatgatcg ccatgatcgc ttctggcgga ctcgtaacat ggattctctt aaccgatggc
       61 ttacgagata tttcttttca gttttctgaa aaccttttcc ccgtctatat gcaggaattt
      121 ggcggtttgt cactgcagca gatcggctgg gtcacttcgg tttttggaat ttgcatgatg
      181 ttgacgacca tccccggcgg ctggctttca gataaggtgg gggaacgagt gggaatcgca
      241 gccggcatga tactcatgag cggagcctta ttcctcttga tcaacattcc tgctggaatt
      301 cagtggctct attatgtagg ctggggattg gcgggtgtcg gcattggcat acttactcct
      361 gcctatcagt cattgatcag caaagccgtt ccggcacaca tgcgcgggac tgcctttggg
      421 ctgttcagca ccagcctggg cgtaatctcc ctcccctccc catggatcgg cgcgcagctt
      481 tgggatcgcg taaacccgca gttcccattt atggtcacgg cgattgtttt attgttatcg
      541 gtaattccaa tttggataaa gttcaaactc cccaaaacgg ttgaaaatgt acgtgtcccg
      601 tcagaggaac aagcagcagt tacaactgcg taatgcattt atgcatcgga ggtacttatg
      661 gcatctgtag aagaacgaat gaagattttg aaaatgatcg aggaaggaaa actgagcgca
      721 gaagaaggca caaaactgct ggctgcgttg agtgaacgac ggggtccgcc cgctcgcatg
      781 cctgggatgc ccggtcccgc gcgctggctg cggattcgcg taacggatac gcgatctgga
      841 cgctcgaaag catcggtgca gatcccgctt gcgttggtgg atgccggact gaaaatcggc
      901 gcgcactttg cccctcaagt cgagggtgtg gatatgtcca atgtgatgga agcgttgcgt
      961 tccggtgtca cgggcaaaat cattgatgtc accgacgacg aggatggcga gcacgttgaa
     1021 atctacgtcg aatagccccc ggttcgtcgt gacaaattca ctttaactgg agaataaatg
     1081 gaaaccaata caaaccgtcc gcgcgggatg ttcggtttta cagttgtttg gcttgggcag
     1141 atcgtttcgg tccttgccag ctcgatgagt caatttgcgc tgacgatctt catgtatgaa
     1201 gagacaggca gcgctaccgc cctgggcttg atgcaggtct ttttcatcac gccattcctg
     1261 atcatctcgc cgattgccgg cgtaatgatc gaccgctata accgcaagat gatgatgatg
     1321 gtgagcgatt tggccgccgg aattgcaacg cttgcgatct tagctttcca ggcgttgggt
     1381 ctactggaat attggcacct ttatgcggct tctattatct atgggttggg aatggcgttt
     1441 caatggcctg cctactctgc agcaatttcg acgatggtac caaaagaaca actgggacgt
     1501 gccaacggca tgatgtcact gatcgaagca ggtcccggcg tgattgcccc cattctggcg
     1561 ggcgctgttc tcccgctgat cggtttgacc ggtattttgg tttttgatgt agttactttc
     1621 ctggtggcga ttggtgcact gctggttgtt catatccccc aaccaccgcg tacgactgaa
     1681 ggttctcagg caaagggagg aatgttgcag gaggctgcct atggcttcaa gtatattttt
     1741 gcccgcccaa gtttgttggg attgcagatg attttcttcg ccggaaattt atttgcaggc
     1801 attgcgttca cgttgcttgc cccaatgatt ctctcacgca ctgggaacga tagcctgatg
     1861 ctaggctctg ctcaaaccgc gggcgcagtc ggcggcttag tggggggtgt tctgatgagt
     1921 gcctggggtg gtttcaagcg acgcgctcat ggggtgctgt ttgggtggat ctggagtgga
     1981 ctcacgatgg ctattgtcgg attcgccggt ggactcccta tgtggattgt ggggctggca
     2041 ctaacagctt tagcgggtcc gttggtaaac gcgtccaatc aggcgatctg gcaatcgaaa
     2101 gtcgcgccag atttacaggg acgtgtgttc tcggcacgac gcctcattgc ctggtttaca
     2161 aatccgattt ctcccatcat cgctggtacc ctcgcagact atgtccttga accggcggct
     2221 cgcgccgaga ccggactgcc tgcgaccttt gcctggctgg tcggaaccgg accaggatcc
     2281 gggatgggac tcttgattgt tttttgtggg gttgcttctg ctgtggttgg tcttttaggg
     2341 tattttattc cagccattta caacgcagaa acaatattgc cagattacga cgcgcttggg
     2401 cccgcaaaac ccgaacccgc ctaaactttt gaaatgacac tacaagaaaa tagccctttc
     2461 ttagggttgt tttcttatta acgctgtgtt atgcttttcc gagaggagaa tgacaggttt
     2521 ggattttttg attcgacgcc cgaatggaat gcggggattt tatgtgatct ggagcggtca
     2581 gatgatttcg ggcatcgctt ccaatattac gttttttgca ttgccaattt ggatcctaaa
     2641 tatgaccgat atcagcggtg gtgcgctcgg tctctgggag tctttttatt ttgggtccta
     2701 tctggttgtc gttctggttg cgggtgtctt cattgatcgt tacagccgca agcgaatgat
     2761 gcttgtctat gatttccttt cgctttctgc gactgcaatt ctgctggttc ttcaatcaac
     2821 tgacaatatg gaagtatggc atttgtatat tgcggccatc tttcagggaa ttggatttgc
     2881 tttccattcg ccttcctatt cagcagcgat caccaccttg gtacccagaa gacaatacgt
     2941 gcgtgccaat ggtttgatgt ctttgctcta tgatgcaccg gagattttcg gcccggtcct
     3001 tgcaggggta ctctacctca cgatcggact gggaggtatt cttgccctta acttgcttgc
     3061 ctttgttgta tccattgggg cattgttatt tgtagagatt ccccctaccc cccatacacg
     3121 cgaagggcaa atgaatcagg aaagcttctg gagcgaagcc atttacggca tcaaatacat
     3181 cctcagacgc cctggcttgc ttggaataca actcatcttc ttcttcggta atttcttttc
     3241 ggggattgca ttatcggtca ccgccctctt caccatgatt ctgttgcggt cgggtgatgc
     3301 cagcgcagcg ggaacggttc agtctcttgg tgcgttggcg gcagtcgttg caggtgttta
     3361 tctaagtaca ttggggggca ttaaacggcc ggttcgagcg attttgctcg gatgggtcat
     3421 atcgagtctg ttcggtctga ccttgctagg tgtgggacag gtattcatcg tctgggcgat
     3481 cgccatggtc cttaacacga tatttgaacc tgttgtgaat gtggccgtcg agaccttcat
     3541 gcaaacaaaa atccctccgg atttgcaggg acgcgtattt tctgccagcg acttccttgc
     3601 gcaggcgatg gtccccatca cacctttgct ggcaggattc tttggtgaag gaatcttcga
     3661 acccgccatg gcggagggag gtccccttgc caatatcttt gggtggctgg ttggcgtcgg
     3721 acccggctcc ggtttcggat tgatgattct gctgtgtggt attggtggaa cgttgatcgg
     3781 gctctctggc tatttgattc ctgcgatccg caacgtggat gattaccttc ccgattacac
     3841 tcctctgcct ccagccgggg tcgtactccg cattcggcca cggacagata aacgaaaagc
     3901 ccgagactct gcaaacaagc gccggatcaa aaaaactgtg aacaaatcca ctcgagaagc
     3961 tggcgaagaa agaataaaca ttgagccgga agaaaaaaga gactgagaaa actcagtctc
     4021 ttttttgtag aatggtcgcc agatctttta cggcttataa gccgtgatct ttgcactgta
     4081 cactgactta tatacatcat cacgtgagag ggtcttcagt tcaatcgtat cacccaaatc
     4141 gttgatggct gagtcaaccg tcgcctggtc gaaaaatacg ggcttgatgg aaatattctt
     4201 gaaacctact gcttccatca tgcctacata atcttttgca tctaccgcac cggatacaca
     4261 accggcccag gcactcaggc tttgcttgat ggatgctggc agcggaccat ccgtcacaat
     4321 atccgagaca gcaagtttgc cgccagagtt caacacacga aacgcctctg aaaaaacctt
     4381 cgatttatca ggcgagagat tgatcacaca atttgagatg ataacgtcaa agctatccga
     4441 ttcaacaggt aaatcctcaa gataaccttg tcggaattca acgttattga gattcaatcg
     4501 ttgtgcactg gcccgggcac gttcgatcat ttgcggcgtc atatccacac caatgacctt
     4561 gccggtctcg ccaactttct tcgcagcgaa aaagcaatca agtcccgcgc ctgagccgag
     4621 gtcaagaacg gtctgaccag gttgcaatgc agccagtgtg attgggtcgc cacagccata
     4681 gctaattgca gactcaccct cgggaaggat ggaaagcaac tccgatgggt agagattgct
     4741 atccgggcca cagcagtccg acgacggtcc acaacaggaa gcgttgtttg aaatcctttc
     4801 tgcataatgc tctcgaacag cttcgtggat aggggtgttt gtagtcatgc tggaatctcc
     4861 ttttgattta ttgaaatcta aataacaaac aagttatagg caaagccaag aagaatggat
     4921 ccaagccaga gtgtgccgat aacgatacca acaactcgcc aacgcgcaat ggtcagtgca
     4981 cctcccagcg cacccaggga tgtgccagcg cccgtgataa gaaaagccag ggctgccccc
     5041 tcactcatcc cgctctccag catcgcgcgc acaagaggca gagatgcttc tgtattgatg
     5101 tagaagggca gcccaagtgt ggcggccaat ggaatgctat aggcatgtcc ggtgccgaac
     5161 aagcttgaaa tccatgctga agggatcaag ccattgagca gatatccaat aaacgtgaaa
     5221 ccaacgaata gtggcagcaa gctcttactc acgttgaatg tctcctgagt gaacagccgc
     5281 aagctcgccg gtgtttttat cggctcagcc agagccggtg gaagaacagg tagtccaatc
     5341 ggtgccggcc cgcccataat tcccagggaa agtgatgagg cttttggggg cgccattcga
     5401 acctgattgg caagccagcc gcgtccttct gcaaaactgg caatggctcc gcccgtcaga
     5461 cccaacagga tggacgcaaa aaagaaagcg atagcaaagg gccagccgaa caatcctgcg
     5521 ctataaaaga gttcctgtgg cgatgtgagc ggggatgcaa caaggaatgc gacgatcggt
     5581 gcccagggga tcgtggatgc catcatgccc agcacaatgg ccgtcgttcc gcatgaacaa
     5641 agcggagtgg ccaccgcaac accggtagac atgatcacgc tgtttttcgt attgcgacgc
     5701 aggaaacgcg cgatggcgtc ttcgtccagg tacaccttga tggcagcgct aatgaggatg
     5761 ctaagcaaaa gcagaaccca gttatttgcg aaagtttcaa ataacttatt ggttgcatat
     5821 tgaagcagag taatgatgtc catgtcttgt ccttcaaaat cgggtttgat gcgaattggt
     5881 gattaaactt gtcatataga agactgtcta tgtatcttaa taaaaaaatt ttacaatgcc
     5941 tctctgctca ccgcgcgatg aacagagaca cttacctagc agcagcctcc gccgccacaa
     6001 caaccgcctt cgcaatcgcc gtcgcagttg ttaatcacga ggttcaattc gttcatagct
     6061 tttacctcct ttcaaataga tatgtgtcga tgcatctggg caaaaaaaga ggtgcctatg
     6121 tcgaaacgac tttaagcacc aactctgttg ctttttcttc gggcgcaaac ttgatcatca
     6181 attgtccaca acagacagct acacattcct ggttgagtgt atagaacgtc tgcttgcctt
     6241 cttcacgaat atcgaccaga ccggcatcgc gcagaatagc aagatggtgt gaaaccgtag
     6301 gttgagaaac tccaactttc tcgacaattt cactgacaga taacgtgcaa caacaacact
     6361 cgttcatgat cttctgacga gtttcatccg cgatggcttt ggcaaactga actggattaa
     6421 gaggagtttt catgaaaatg cctcgctgaa ttcttcgcgc agaaattcct gcgttccttc
     6481 agggcctgtg agaattaaag agataggatc attcgttgaa ggtattttaa gtgtaaactc
     6541 taaaaatgaa cagcaccgtc tctcctctga gataaacctt gcaaaatcca ggataccctc
     6601 tgagccatca ttgggaaaga gaaactcata gccattctgt gtttcgtgaa tactttgcac
     6661 ggcctgaaag agtcgtctgg tggtttggat gtggctctca cgctccattg gcgtaaagac
     6721 atccatatta cagaaaatga tcgtatcgcg aggtacagaa ttcatatcga caaccgtcta
     6781 tttgttatta tatcgatgat cgtctatttg tcaagaggtc gaaaatcgtt gacacagagg
     6841 aaaatccatt ctataacagg aattcttaca aaggagtttt ttcatggcag ccaatgcggt
     6901 tcgagcgctt attctttcag gtggtggtgg gcggggtgca tttcacgctg gggtatataa
     6961 gtatttgatg cggcaacaca agctcaacct ggatgcggat catgatgatg tttgggatcc
     7021 acaaattatt gttggcacat ccattggcgc tgttaatggt gccgcgattg ctcaggggat
     7081 gtctgccgac cgacttgtgg aagtgtggaa gtcactggag gagaaagata ttcagggctt
     7141 gccgccggga atgcgtggca tcgcgcgctg ggtcgcgggg actattttca aagaagtgat
     7201 ggatacgaag cttccgcaga tccatccgaa ggaagccacc tcaccgattc ccaaggaatt
     7261 ttggccaccc ctgcctctac tcccaaactg gatcgctgaa
//
LOCUS       DASTJP010000721         4175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1013948, whole genome shotgun sequence.
ACCESSION   DASTJP010000721 DASTJP010000000
VERSION     DASTJP010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013948"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFQ23_21080"
     CDS             <1..347
                     /locus_tag="VFQ23_21080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9909156.1"
                     /translation="SLGLKICDRAKVLSEALRVLRPGGRFIALEASDIVWSWLHRAYL
                     HYMMFTMPVIGWIATGGDSSAYKYLLKGIRGFPGAEAFAAELSTVGFEDISFERLSLG
                     IVAIHRARKPSF"
     gene            571..1110
                     /locus_tag="VFQ23_21085"
     CDS             571..1110
                     /locus_tag="VFQ23_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909157.1"
                     /translation="MALYFENLDERTRGLMLDEMKYDITNNQLHISPFLSGQGQRDYA
                     SLLQEAIQHGDDETLAQNLREHRRINRTQPRRQPKASGYSIAATPENAAEILAESEFN
                     RYYIRGVARCAIEDGIQELVIYRAKPARNPRPESEARVETSISPQDLLDDLRSHPGDE
                     PPALGVPSGPNSGLSVRLP"
     gene            1268..1747
                     /locus_tag="VFQ23_21090"
     CDS             1268..1747
                     /locus_tag="VFQ23_21090"
                     /inference="COORDINATES: protein motif:HMM:NF025686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4385 domain-containing protein"
                     /protein_id="HET9909158.1"
                     /translation="MTFDYSIDFENIDFRKHPELYRVGKGEQGVLLVEPYKSEILPHW
                     RFKTPDIARKSANKIYKMFLDYKKMNDFVGMDMARKFLQMGYTRSRRYANHKSGRKYA
                     KDSKEVLPREEDKVKAESAKIFYEKWHKAKADKKYLERKKNHQEFYESKVKVKERAG"
     gene            1860..2396
                     /locus_tag="VFQ23_21095"
     CDS             1860..2396
                     /locus_tag="VFQ23_21095"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9909159.1"
                     /translation="MDSINRNQPEDNREDLQGQEAVNKIKEMVKHAENCWFCSSVATG
                     ESNGDRPMNVRQVDDEGNLWFLSASDSRKNEELGLDSEVKLYFQGSKHSDFMQLNGQA
                     SITRNKEKIKELWSPLVQTWFTEGIDDPRVTVIQVTPTAGYYWDTKHGNAVAGVKMMI
                     GAALRKTLDDSVEGTLDV"
     gene            complement(2554..3366)
                     /gene="mutM"
                     /locus_tag="VFQ23_21100"
     CDS             complement(2554..3366)
                     /gene="mutM"
                     /locus_tag="VFQ23_21100"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET9909160.1"
                     /translation="MAEVPEVETLVRDLREAVVGRALLTTDVLLPATVRYPAVAEFSE
                     LLTDRTFLDSQRRAKYILLPLSGDLLLAVHLALSGTLTLVPADEPRLPETVIIWHLDQ
                     AQELRFTDKLGYARAAVGSPDILAERLDLNSLGPEALDPNFNVDVLTRQLAKRRGVLK
                     TVLLNQRVLAGLGNRDADESMWLAQIDPRRPAASLTSEEHIRLYDAIKNVLAEGIALR
                     GTQRDLFGRRGEAMHRRQIFEKARQPCPRCGTLIAYLRIGGRNTYYCPNCQG"
     gene            complement(3442..>4175)
                     /locus_tag="VFQ23_21105"
     CDS             complement(3442..>4175)
                     /locus_tag="VFQ23_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909161.1"
                     /translation="RQIAYGEGAVWALGTDHTALARIDPLTYSVIAEIDLSSLQMPEY
                     NLVLIAAGEGSVWITDQTAVIQIDPKTNQFVGEPLFTGEEIIAVALGHGTFWTGSHDD
                     GIVARVDPATHKVVASIEVGFSVHGLAVDDESAWVLDEHGSAVVRIDSQTNLMQERIP
                     IDFIGANLAAGAGSVWVAPAARDSGRSIGNDGIARISADEKQIVETIHVGGVPESEYY
                     AAYYADGSVWVLVVTPQMSVVQIAP"
BASE COUNT         1129 a         1028 c         1054 g          964 t
ORIGIN      
        1 tttcattggg gcttaagatt tgtgacagag cgaaggtttt aagcgaagca ctacgcgttc
       61 tccgtccggg cggcaggttt atcgcattgg aagcctcaga tatcgtttgg tcgtggctgc
      121 atcgcgctta tctacactat atgatgttta ccatgccggt cattggttgg atcgccaccg
      181 gaggcgactc ttctgcatac aagtatctgc tgaaaggtat ccggggattt ccaggcgcgg
      241 aagcgttcgc ggccgagttg tctaccgtgg gatttgagga tatttcattc gagcgactct
      301 ccctcggtat cgtggcaatt catcgcgcca ggaaaccatc attttaataa actcaaaaag
      361 cttgtcttgc ccttgtcttt tgcatagatt tctagtacaa ttgttctatc cattgcataa
      421 ctcgtcaaaa ttgaacaggc acatcataag gggatgttga ttcgtcgaca cttctcaagg
      481 aggaactggg cacacatttc ggtcccgtaa catctaacta aaaaggcacg ttaaattaac
      541 tgaacacgta caggaaagta gtaggagaca atggctctat attttgaaaa tctcgatgag
      601 cgcacgcggg gactcatgct cgatgagatg aaatacgaca tcaccaacaa ccaattgcat
      661 attagtccct ttttgagcgg tcagggtcaa agagattatg cttccctttt gcaggaagca
      721 attcaacacg gcgacgatga aacgcttgct caaaacttgc gtgaacaccg gcgcatcaac
      781 cggacccagc cgcggcgcca gcccaaagcc agcggatatt ccattgctgc cacacccgaa
      841 aacgcagcag aaatattagc cgagagcgaa ttcaaccggt actacattcg cggagtggct
      901 cgctgcgcga ttgaagatgg cattcaggaa ctggtgatct atcgcgccaa accagctcgc
      961 aaccctaggc ctgagtcgga agcgcgcgtg gagaccagca tctcgcctca agatttgctg
     1021 gatgacttac gatctcatcc cggcgatgaa ccgccagccc ttggcgtacc atccggaccg
     1081 aactccggac tcagtgttcg cttgccctga attctgtaaa tttcacttta ttcgtaatgc
     1141 cgcctgacat ccaatgctca ccgtgatgta aggcggttgt gatttaataa gtgtttcaga
     1201 caagaggaac gaatcctctt aagcgaccgc tttgcgcgga gtcctagaaa agacacggta
     1261 cgaaacaatg acattcgatt attccatcga cttcgaaaat attgatttca gaaaacatcc
     1321 ggagctttac cgcgtgggca agggcgagca gggcgtgttg cttgtcgaac cctataaaag
     1381 cgagatcctt ccccattgga gattcaagac ccccgatatt gcgcggaaat ctgctaacaa
     1441 aatttacaag atgtttctcg actacaagaa gatgaatgat ttcgtcggca tggatatggc
     1501 tcgcaagttc ctgcaaatgg gatatacacg ctcacgacgc tatgctaatc ataaatctgg
     1561 gcgcaaatat gcaaaagaca gcaaagaagt tctgcctcgc gaagaggaca aggtcaaggc
     1621 ggaatctgca aagatttttt atgagaagtg gcataaggca aaagcggata aaaaatattt
     1681 ggaacgaaag aagaaccacc aggaatttta tgagagcaaa gttaaagtga aggaaagggc
     1741 cggttaaagg aacggtcaaa catccagcaa ggaccattca gacaccgcct tcaaaaatcg
     1801 tgtcatagtt catccctgcc tcgttaatcc aaatacaaaa ccaatcagga gatgaaggaa
     1861 tggattcaat caacagaaat cagcccgaag ataaccggga agatttacag gggcaggaag
     1921 cagtcaacaa gatcaaggag atggtgaaac atgcagaaaa ttgctggttt tgctcatcgg
     1981 tcgctacagg cgaatcaaat ggggatcggc cgatgaacgt ccggcaggtg gatgacgaag
     2041 gaaatctttg gttcctgagc gcgagcgaca gtcgcaagaa cgaagaactt gggctcgact
     2101 cggaagtaaa actatacttc cagggttcaa agcattctga ttttatgcaa ttgaacggac
     2161 aggcaagcat tacacgaaac aaagaaaaaa ttaaagagtt atggtcccct ctggtgcaga
     2221 cctggttcac tgaaggcatc gatgatccgc gtgtcacggt catacaggtc acacctacgg
     2281 ctggctatta ttgggatacc aaacatggaa atgctgtggc cggagtgaag atgatgattg
     2341 gggccgcgct tagaaagaca ctggatgatt cagtcgaagg aacactggat gtttgagtgg
     2401 ttgataattt gtagttgata aaaaaacgga tggaagccat tgcgcgccat ccgttttgct
     2461 tattgttcat ccaagagttt tgcatcgtgt ccctgcgcag gattctttct ggaacacaat
     2521 ttagtgaact gaaagatcag cgagacagat atttcaaccc tgacagttgg ggcaataata
     2581 ggtgttccgg ccgccgattc gtaaataggc aatgagtgtg ccgcagcgcg gacagggctg
     2641 tctggccttc tcaaagattt ggcggcgatg catagcttct cctctgcgcc cgaagagatc
     2701 gcgttgtgtt ccgcgtaagg caatgccttc agccaaaacg ttcttaattg catcataaag
     2761 tcggatgtgt tcttcagagg ttagtgaggc tgccggacga cgcgggtcaa tttgagccag
     2821 ccacatactc tcatcggcgt cgcgatttcc caagcctgcc agcacgcgct ggttcaacag
     2881 cacggttttt agtacacctc gacgtttcgc aagttggcgc gtgagtacat cgacattgaa
     2941 gtttggatcc agtgcttcag ggccgagtga attcaggtca aggcgctccg ccaggatatc
     3001 aggtgatcca actgccgcgc gggcatagcc aagtttgtcc gtaaaacgca gctcctgcgc
     3061 ctgatccaga tgccagataa tcaccgtttc aggcaagcgc ggttcgtcag ccgggacgag
     3121 ggtcaaggtg cctgacaatg ccagatggac agccagcaga agatcgcctg aaagaggcaa
     3181 caaaatatac tttgcgcgcc gctgagaatc cagaaaggta cgatcagtta ataattcaga
     3241 aaattcagct accgcagggt aacgcaccgt tgccggtaac agaacatcgg tcgtcagcaa
     3301 agcccgaccg acaaccgcct cgcgaaggtc gcgtaccaga gtttccactt cagggacttc
     3361 cgccatgata catataacga acttccggaa gcttcgtcat gggaaaagct ttatacaaag
     3421 actgggaaat ttgctgttga gttagggtgc aatctgcaca acactcattt gtggcgtaac
     3481 aaccagaacc caaactgagc catcggcgta gtaggcggcg taatattcac tttcaggcac
     3541 tcctcccacg tgaatcgttt ctacgatttg tttttcatct gcgctgatgc gtgcaatgcc
     3601 gtcgttgccg atagatcgac cactatcccg cgcggccgga gctacccaga ccgatcccgc
     3661 gcccgccgcc aggttcgcgc cgataaaatc gatcggaatt cgctcctgca ttaaattcgt
     3721 ctgtgagtca attcgtacca cggcggaccc gtgctcatcc agcacccagg cggattcatc
     3781 atccactgcc aacccgtgta cggagaagcc cacctcaatg ctggctacca ccttgtgagt
     3841 cgccgggtcg acacgtgcca caattccatc atcgtgacta ccggtccaga atgtgccatg
     3901 gccgagagca acggcaatga tttcttcgcc agtaaatagc ggctcgccca cgaattgatt
     3961 cgttttcggg tcgatctgaa tgactgcggt ttgatccgtg atccagaccg atccttctcc
     4021 cgcagcgatg agcacaagat tgtattcagg catctgaagg ctactaaggt caatttctgc
     4081 gatgaccgaa tacgtcagag gatcaatcct ggccaaggca gtatggtctg tgcctaatgc
     4141 ccagactgct ccttccccat aagcgatttg gcggg
//
LOCUS       DASTJP010000722         5418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_222007, whole genome shotgun sequence.
ACCESSION   DASTJP010000722 DASTJP010000000
VERSION     DASTJP010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222007"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="VFQ23_21110"
     CDS             <1..1565
                     /locus_tag="VFQ23_21110"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9909162.1"
                     /translation="FIWLFTNRKWNERWGEMTGKSLLKESPWVVETGMFDRFVSAMQS
                     AVPLVQEQYYADERFNGWFIQSLVLVEDGLLVTTFDITQLKQAEEALHRSKSQLAKEL
                     EDTQQLQKISSQLIHEDNIEVLYRQILQAAISLMHSDMGSLQMSDPETEQLRLLAWKG
                     FHPESAAYWDTITDESSTLCGRALRTGERQIVPDLNSPDFVTDSENRKQYQLSGILAM
                     QSTPLISRYGRPVGMISTHWSQPHVPGEHELRMFDVLARQAADVLERARAEEALRESE
                     QRLKALNETLEQKVHEKTIEVRRLASDVVTAIQRERQRISHVLHDDLQQRVFAVQMQM
                     SFLKDALAAENESARQEASDIERELAEIVKITRDLSLNLSPPILPGEGLAHALEWFAS
                     RVREQFDLAVEIQTTEPYIIANEELHVLLFNFVRELLFNVVKHAGASQAVIRMESSDQ
                     HLRIEVHDNGKGFPIDAPDGRRSPGNDLPRSLGLPTIRHQLSLLGGKIEINSKPEAGT
                     QVILTVPVIEGQ"
     gene            complement(1658..2470)
                     /locus_tag="VFQ23_21115"
     CDS             complement(1658..2470)
                     /locus_tag="VFQ23_21115"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9909163.1"
                     /translation="MSGSLSLLSMNCFGAPVPTTRRRLQALAHHLEQSSLQIVCLQEV
                     QLLAYQNLLFQACASFPFQAYEPHLHCPKGGLVTLSRTPLTTQRFETFTEQGPWYLPT
                     AMDRFLRKGMLSSSLQWGEVPVVVINTHIIANYSGDWERKGVFTRMQEKQLRQLADTV
                     AAQPSHALVAVVGDFNLPRGSRLYHDFLQQTGLVDALAGDKRPTHRPPPGVPAHYSLP
                     IDFVFMRVPGSLSLQITGDLRFASKLDITHRYQDYLSDHNAIEVGVTLNMPA"
     gene            complement(2688..3143)
                     /locus_tag="VFQ23_21120"
     CDS             complement(2688..3143)
                     /locus_tag="VFQ23_21120"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909164.1"
                     /translation="MITIGFLADYPDTIPTLAKWFREQWPDYYADLSQEELEKDFLED
                     TSRDRLPSRLVAFESNELVGTIILRERESLAEFGPELGGLFVLESHRRHGIGTALVRA
                     GMKLASEQGYETVVATTVVAAGILESLGWEFIKTVVHEDGELALYQCKL"
     gene            3418..4491
                     /locus_tag="VFQ23_21125"
     CDS             3418..4491
                     /locus_tag="VFQ23_21125"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET9909165.1"
                     /translation="MENQMMQALVWLGPRDMVQRAEPIPKLATDEVLIAVGAVGICGS
                     ELGGFLGHNSLRVPPLIMGHECAGQVVQVTGGTFATGKKASVGTRVTFNPLVVCGTCD
                     RCISGKSNLCRRRQLVGAHRPGAFAQYVAVPARQCYPLPESSPALAPGASVSLIAGSL
                     AEPLACSIRAVALSGVQPQESLLILGAGPIGLCAVAAARAQGIEQIIVSDVAPQRLEI
                     ARRWGASDIVNARQQDVVAFVQDNFLGGVDRVIDAVGATPVRAQAIRAVVPGGRVVFI
                     GLHDEESVLPANYIIRQEITVTGSFAYTDDDFAQAIDLLKQGVVQPSADWLEERPLSD
                     GPAAFAELVDGKASVAKIVLTVS"
     gene            4517..>5418
                     /locus_tag="VFQ23_21130"
     CDS             4517..>5418
                     /locus_tag="VFQ23_21130"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET9909166.1"
                     /translation="MKISAVRPLVLGTPWRDLTFVVVETDDGLRGVGEARMVNHTEAL
                     LGYLREASRNHILGSDPFKIEDLVQRMFRRDFARASEIMTSAIAVVEMACWDIMGQSL
                     GVPVYQLLGGSVRDRIPAYANGWYTVERTADDFRTAVRPVVERGYRALKVDPFGAGFY
                     ELSRAEHNRSIELIEAIRDEVGPDIEIFIEMHGRFNPATAIAMTNALAPFRPGWIEEP
                     VPPENLRALQKVAQAVAPLGIPVAAGERMHTAHEYRELFELQAADIVQADITHYGGMM
                     ATKKLAAWADAYYMLMAPHNVCGPV"
BASE COUNT         1285 a         1351 c         1497 g         1285 t
ORIGIN      
        1 atttcatctg gctgtttact aatagaaagt ggaacgaacg atggggcgag atgacaggca
       61 aaagtttgct gaaggaaagc ccctgggtcg tcgaaacagg aatgttcgat aggtttgtta
      121 gtgccatgca gtcggccgtc cctcttgtgc aagagcaata ctatgctgat gaacgattca
      181 atggatggtt catccagtct ctcgtcctgg tagaggatgg tctcctggtg accacttttg
      241 acatcacaca actaaagcag gccgaagaag ccttgcaccg gagcaagtct caactagcaa
      301 aagagcttga agacacgcag caattacaaa agatcagcag tcagctcatt catgaagata
      361 acattgaggt gctgtatcgg caaattctgc aagctgcgat ctcattgatg cattcagata
      421 tgggcagcct gcaaatgtct gacccggaaa ccgagcaact ccgtctcctg gcctggaagg
      481 gatttcatcc tgagtctgct gcgtattggg acacgatcac tgacgaaagc tccacgctct
      541 gcggcagggc acttcgaacg ggcgaaaggc aaatcgtgcc agatttaaac tctccagact
      601 ttgtgacgga cagtgaaaat cgcaaacaat atcagctcag cggtatcctg gcaatgcaat
      661 ccaccccgct catctcgcga tatggtcgcc cggttgggat gatttcaacg cactggagcc
      721 aaccgcatgt ccccggcgag catgaattga ggatgtttga cgtgctggcg cggcaagctg
      781 ccgatgtatt ggaacgcgcg cgtgccgaag aagcgttgcg ggaaagcgag cagcgactga
      841 aagcactgaa tgaaactcta gagcagaaag tgcatgagaa aacaatagaa gtgcgccgtc
      901 tcgcttctga tgttgtcacc gccatacaac gcgagcgcca gcgcatctcg catgttttgc
      961 acgacgattt acagcagcgt gtttttgcgg tccagatgca aatgtctttt ctcaaagatg
     1021 ccctagctgc tgaaaatgaa tcggcgaggc aggaagcgtc tgatatcgaa agggaactcg
     1081 cagagatcgt gaagatcacg cgtgatctca gcctgaacct gagcccgcca atcctgcccg
     1141 gagaaggtct ggcacatgcc cttgagtggt tcgcctccag agtgcgcgag cagtttgact
     1201 tggcagttga aattcagaca actgaaccgt atatcatcgc caatgaagag ctgcatgtgc
     1261 tcttgttcaa tttcgtacga gagctgttgt tcaacgtagt gaaacatgcc ggggcgagcc
     1321 aggcagtcat tcggatggaa tcatcagatc agcatctgcg catcgaggtg cacgataatg
     1381 ggaagggttt cccgatcgat gcacccgacg gccgaaggag cccgggaaac gacttgccga
     1441 ggagtttagg tctcccgacc attcgtcatc agttaagtct tctcgggggc aaaatcgaga
     1501 tcaattcgaa gcctgaagcg gggacgcagg tgattcttac agtccctgtg attgaaggac
     1561 aataagaaat ttccttctga tatgttgtat atcttttaga ttagtgtgca gacgagatgg
     1621 gatcctttgg gacgcctgca tcggtttgca cccggcatta tgccggcata ttgagcgtaa
     1681 ccccgacctc aatcgcattg tggtctgaaa gataatcctg atatcggtgt gttatatcga
     1741 gtttgcttgc aaaacgtaaa tcaccggtga tttgcagcga gaggctgcca gggaccctca
     1801 taaaaacaaa atcgatcggt agggaatagt gtgcgggtac gcccggaggg ggacgatggg
     1861 ttgggcgctt gtcgccagcg agtgcatcta ccaaaccggt ctgctgtaga aaatcgtgat
     1921 agagcctgct gccgcgcggc aagttgaagt caccgaccac agccaccagc gcatgggaag
     1981 gctgtgccgc tacggtatct gccagttgac ggagttgctt ctcctgcatg cgagtaaaaa
     2041 cacctttgcg ctcccagtca ccactgtaat tggcgatgat gtgggtattg atcactacta
     2101 cgggcacctc tccccattgt aacgaactgc tcagcatccc tttgcgcagg aatctatcca
     2161 tggccgtcgg aagataccag ggtccctgct cggtgaaggt ttcgaagcgc tgggttgtca
     2221 atggcgtacg ggacaaggtg accaacccac ccttagggca atgcagatgg ggctcatagg
     2281 cctgaaaagg aaagctggca catgcctgga acagcaggtt ttggtaggca aggagttgca
     2341 cctcctgcag gcaaacgatc tgcaggctgc tttgctccag gtgatgagca agggcttgta
     2401 atctgcggcg agtggttggc acaggtgcgc caaaacagtt catgctgagt aacgataatg
     2461 atccggacat tttatgacta cgttttcctt ctttggtgaa acgggctgga aggtatttag
     2521 tttactacat ctggaccgcg gcatattttg cggaaaggtg ggtcttgcta ggtggtttcc
     2581 gaaacgaatt catgaaaata cctcgcgtag acttctgcta ccatcatagc tgaattatcg
     2641 ataatccatt cgtcttctat atagtttgac tcatttccgg gcagcactta tagtttgcat
     2701 tgatacaacg ccagttcccc atcttcgtga acaactgttt tgataaattc ccaacccagg
     2761 ctttccagga ttcctgctgc tactaccgtt gttgcgacta cggtctcata cccttgctcg
     2821 ctggctaatt tcatgccggc tcgaaccaac gctgtaccaa tgccgtgacg tcgatgagac
     2881 tcaaggacaa ataatcctcc taattcaggc ccgaactctg ccaggctttc gcgctcgcgt
     2941 aggataatcg tgccgacaag ctcattcgac tcgaaggcaa ccaggcgcga gggcaggcga
     3001 tcacgagaag tatcttcaag aaaatccttc tccagctcct cttgtgacaa atcggcataa
     3061 taatcaggcc actgctcgcg aaaccacttg gccagggtgg gtatggtatc agggtaatct
     3121 gcaaggaaac caatcgtaat catgtgacct gtcaatctat aaaacaatta gtcttcaaaa
     3181 tgttggacaa aatacctacc agagattgtt gcgaaaccaa tttcaccaaa aattattata
     3241 gcattcgtgt gagcacgctc attttttctt atcctgcgcg ctttactgat gctcagtcgg
     3301 ttggctattt tttcgcttca gtacggcgac agtaatagag ataaactaca tataataatg
     3361 cagtagcgca gtgacctaac cccacaggat cgtcagcaag aaccaggaga aaacacgatg
     3421 gagaatcaaa tgatgcaggc actggtgtgg ctcggtccac gcgatatggt acagcgtgct
     3481 gaaccaatac caaaactggc aactgacgaa gtattgatcg cagttggcgc ggtgggtatc
     3541 tgtggttcgg aactaggcgg ctttttggga cacaatagcc tgcgcgtccc gccgctcatc
     3601 atggggcatg aatgtgccgg acaagtcgtt caggtgacag gtgggacatt tgcaacgggt
     3661 aagaaagcct cggttgggac ccgcgtcacg ttcaatccgt tggtggtttg tggaacctgt
     3721 gaccgttgta tttctggcaa atcaaatcta tgtcgtcgtc gccaactggt cggcgcgcat
     3781 cgcccgggtg cattcgccca gtatgttgct gtaccagcgc gccagtgcta cccgctccct
     3841 gagtcttccc cggcacttgc gcccggggca agtgtgtcgc ttatcgcagg atcattggcc
     3901 gagccactgg cgtgttccat ccgagccgtc gcgctcagcg gcgtgcaacc acaagaaagt
     3961 ctgctgatcc tcggggctgg gccgatcggg ctttgcgctg tcgccgctgc acgcgctcag
     4021 ggcattgagc agattattgt cagtgatgtt gcgccacagc ggctcgagat tgcacgacgc
     4081 tggggtgcga gcgatattgt caatgcgcgc cagcaggatg tagtcgcttt cgtgcaggac
     4141 aacttcctcg gaggtgtcga tcgtgtgatc gatgcagttg gtgcaacgcc cgtgcgcgcg
     4201 caggcgattc gggcggtggt accaggtggt cgcgttgtct tcattggcct tcatgatgag
     4261 gagtcggttc tgcctgccaa ctacatcatt cgccaggaga tcacagttac cggcagcttt
     4321 gcttatacgg atgatgactt tgcacaggcg atcgacctgt tgaagcaggg tgtcgtgcag
     4381 ccgagtgcgg actggctgga agagcgccca ctgagcgatg gaccagcggc atttgctgag
     4441 ttagtagatg gcaaagccag cgttgccaag atcgttctga cagtttcttg aaaaatccac
     4501 ttagacggag tctgcaatga aaattagtgc tgttcgccca ctggtattag gcacgccctg
     4561 gcgagatctg acttttgtgg ttgtcgagac cgatgatggg ttgcgcggcg tcggcgaggc
     4621 acgcatggtg aatcataccg aagcattgtt aggttacctc cgtgaagcca gccgcaatca
     4681 catccttgga tccgatccct tcaagatcga agatctggtt cagcgtatgt ttcggcgcga
     4741 ctttgctcgt gcgagcgaga tcatgacctc cgccatcgca gtcgtcgaaa tggcttgctg
     4801 ggacatcatg ggtcagtccc tgggtgtgcc ggtctaccaa ctcctcggtg gatccgttcg
     4861 cgatcgtatt ccagcatatg cgaatggctg gtacacggta gagcgaaccg cggatgactt
     4921 tcgaacggcg gtgcgcccgg ttgtcgaacg cgggtatcgg gcactcaagg tagacccatt
     4981 cggtgcaggt ttttatgaat tgtcacgcgc tgaacacaat cgctctattg aactgataga
     5041 agccattcgt gatgaagtcg gacccgatat cgaaatcttt atcgaaatgc acggacgatt
     5101 caacccggcg accgctattg caatgaccaa tgcccttgcg ccattccgcc cgggttggat
     5161 cgaggagcct gtgccacctg agaatctgcg tgccctgcaa aaggttgcgc aggctgtcgc
     5221 tccgcttggt attccagttg ccgctggtga gcgtatgcat actgcacacg agtatcgcga
     5281 actattcgaa cttcaggctg cggatatcgt gcaggcagat attacgcatt acggtgggat
     5341 gatggcaacc aagaagcttg ccgcatgggc agatgcttat tacatgctta tggcaccaca
     5401 taatgtgtgt ggtccggt
//
LOCUS       DASTJP010000723         6886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_275294, whole genome shotgun sequence.
ACCESSION   DASTJP010000723 DASTJP010000000
VERSION     DASTJP010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275294"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFQ23_21135"
     CDS             complement(<1..767)
                     /locus_tag="VFQ23_21135"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HET9909167.1"
                     /translation="MSVIEEVVTKIPDWAGKNISIVPLSGGLTNSNFRVDVDGTPYFV
                     RVPGASTELLAVNRDNEYYNSKAAYEAGVGPKVLYHLPEYSVMVLEFLNGKTMSKDSL
                     NEHGMPTRMAQAIKKLNAGPRFLLDFNMFRLTEYYLGICKDHEINIPNGYAERMPTIR
                     RIEQAMNVSPVATVPCNNDLLAENYIDDGTQLWLIDYEYSGNNDPTFELGNTCQEMQF
                     NDDQIREVCVAYFGHADADKIARMKLNMIMSDVGWGL"
     gene            complement(846..1919)
                     /locus_tag="VFQ23_21140"
     CDS             complement(846..1919)
                     /locus_tag="VFQ23_21140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909168.1"
                     /translation="MSEGFLSLNGVSKQFGENVVVDNFNLDIQKGEFVSFLGPSGCGK
                     TTTLRMVAGFESPTDGTISLDGADITQKAPNQRNMGMIFQSYALFPNMTVSQNIGFGL
                     RVRKASKADIADRVNEMLSLVNLEKHKDKYPYQLSGGQQQRVSLARALAIRPHVLLLD
                     EPLSALDAKIRVSLRDQIREIQKRLGITAIFVTHDQEEALSISDRIVVMYDGKVEQVG
                     TPFEIYNFPQTTFVANFVGSLNTAAAEVIDPSKGLLSLDGVQFSSAAKMDGLKKGDKV
                     RMAIRPERFSFASEGKKANVVDCRIENITFLGSVVRVHILVGSTKFTMDTFNYPSMEL
                     PKIGSTQQVTCSREAVLILAPAE"
     gene            complement(1922..2749)
                     /locus_tag="VFQ23_21145"
     CDS             complement(1922..2749)
                     /locus_tag="VFQ23_21145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9909169.1"
                     /translation="MSIPTTPLKISTATSSDQPVVKKSAIGPWLIYLVTALYFLVPLA
                     TTFYWSLRAEKDKLGLEAYRRLFADSNFLPSFNQSIMNAVAAIFISLLIIVPTAYWVT
                     LRLPKLRPVIEFITLLPFVIPAVVLVFGLIRLYSWPPLLWTATYDSSRILLICVYAAL
                     SFPYMFRSVDNGLRAMDVRSLTEAAQSLGANWSTILLQVIFPNLRVALLSGALLTFAI
                     VIGELTIALFMAQRTLGPYMADLTRSKVYEPAAMTIVAFAITWLAMGLIAYISRERN"
     gene            complement(2746..3660)
                     /locus_tag="VFQ23_21150"
     CDS             complement(2746..3660)
                     /locus_tag="VFQ23_21150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9909170.1"
                     /translation="MGTTANTDPKPVRSTQGGAKRPPRLNAGQWLGVIPFFVFSGLFL
                     IAPTVLLAYRSIENPEGGLTLQYYGQLLDQNAVNAYRISIGISLTTAILGGLLGFLLA
                     YAISIGGLPKPLRSLTSTFSGVAANFAGVPLASAFVFTLGRVGLITTFFEFLGFDLYG
                     SGFSLYSFLGLTLVYLYFQIPLMVLTILPAIDGLKREWREAAENLGASPAQYWRDVAF
                     PVLTPSILGTMILLFGNAFGAHATAYALTGGLFTLATLLIGQQISGDVLNNPGLGHAI
                     AMGMVFIMAFAIFFIYLPLQRRSERWLK"
     gene            complement(3667..4794)
                     /locus_tag="VFQ23_21155"
     CDS             complement(3667..4794)
                     /locus_tag="VFQ23_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9909171.1"
                     /translation="MKTRKYLFQLFSLLIALSLALAACAPAATEAPTTSDNALYEAAK
                     AEGMLTTIALPLDWCNYGAMYEGFKAKYPGIEINPLDPGAGSAEELEAIRANKDNPGP
                     QAPDVVDIGIGLAPDAVEEGLLQAYKVSTWEDIPEGAKDPNGYWYGDYYGVLSLEVNT
                     AEVANPPTEYADLLKPEYNGQVALSGDPLTSNAAALSVWAAGLATGATGPDAANAGLD
                     FFRQLNEAGNFVPIDGDSRTVGIGETPILIDWSYNSIANDIALAGNPDLVTNVPKQGI
                     IAGVYVQGISAYAPHPNAAKLWMEYLYSDEGQLIYASGFCYPIRLDAMNAAGVIPDEV
                     FAQLPSIEGAYFPTVDDITAARAIIAEGWPSVVGVEVKPLP"
     gene            complement(4899..5648)
                     /locus_tag="VFQ23_21160"
     CDS             complement(4899..5648)
                     /locus_tag="VFQ23_21160"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9909172.1"
                     /translation="MAIVPDIQNEMNRLISTAREKSILIRVLGGLGVKLYQKEDHPTF
                     RREYGDLDFIVAGKQRREFEAFMPSAGYSPHKQFNVLNGAQRQIYYHNETEMKIDIFV
                     GSFEMCHKIPLEDRLHVHPFTIPLAELLLTKAQIAELNRKDGLDLASLLLFNATGHDD
                     SKINLSQIAKLCGADWGLYKTTSINLKRVEEIVRDENVKLEDSERSLIQQRVKEIQQT
                     FETMPKSLAWQMRDKVGTRVRWYEEVEEVAR"
     gene            5725..6534
                     /locus_tag="VFQ23_21165"
     CDS             5725..6534
                     /locus_tag="VFQ23_21165"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9909173.1"
                     /translation="MNKSLRDLMHKGLITCPPETSLGNVAMLLTQHHIHALVVVENNK
                     PLGIISDFDLLAGEWLSVDAESLDTMRRMTARDLMTYPIATIESNISAEAAAQQMIEK
                     QVHRMIVIENGEPAGVISISNFVASIAEQEPVKRQTVGDVMSETFLVCREETSVLSAA
                     RTMTQAGWRSIVVVNATGKLLGFITGRDLMHLAGRNVDEKLKVSQCMNRSLLTVDINA
                     SLQEAADLMIQNHRHRVVVIDTNDPDSFPLGVLSSFDIVAEMARPGSIWQS"
     gene            complement(6680..>6886)
                     /locus_tag="VFQ23_21170"
     CDS             complement(6680..>6886)
                     /locus_tag="VFQ23_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909174.1"
                     /translation="IVEWMFDPWLNTGGEGAGLYGISFKNTNSIIFLAACYGLAAAIY
                     YGSKARRKTEGIDLDKVQAEIPVE"
BASE COUNT         1803 a         1741 c         1673 g         1669 t
ORIGIN      
        1 cacaaccccc aacccacatc agacatgatc atattcaact tcatccgtgc gatcttatct
       61 gcatcggcat gaccaaaata tgccacacat acttctctga tttgatcgtc gttgaattgc
      121 atctcctggc atgtatttcc caactcaaac gtcgggtcgt tattgccact gtattcgtag
      181 tcaatcaacc ataactgcgt gccgtcatcg atatagtttt ctgcgagcag atcattgtta
      241 cagggaacgg tcgcgacagg gctgacattc attgcctgct caatccgtcg gatcgttggc
      301 attcgctccg catatccatt ggggatattt atctcatggt ctttgcaaat tccgagataa
      361 tattccgtca ggcgaaacat gttgaaatcc agaaggaaac gcggccctgc atttagcttc
      421 ttaattgcct gcgccatacg agttggcata ccgtgttcgt tcaatgaatc ctttgacatt
      481 gtcttgccgt tcagaaactc caaaaccatc acgctatatt caggcaaatg atataaaact
      541 ttggggccga caccggcttc gtacgcagct ttggaattgt aatattcgtt gtcgcgattc
      601 actgccagca gttctgtact tgcgccagga acacgcacaa aatatggagt tccatccacg
      661 tccactctaa agtttgagtt ggtcaggcca cctgaaagcg gaacaatgga aatatttttt
      721 ccggcccagt ccggaatttt cgttacaact tcttcgatga cagacatgta ctgacctcac
      781 aggttaatta aagatatggt ggttgccggc caccattatc caggaaaacc actataaatc
      841 cttagttatt ccgcgggagc caggatgagg acagcttcgc gagagcaggt gacctgctga
      901 gttgaaccaa tctttggtag ttccatcgac gggtagttaa atgtatccat cgtgaacttg
      961 gtactgccaa ccaggatatg gacgcgaaca accgagccaa ggaaggtaat gttttcgatg
     1021 cgacaatcta caacattggc cttttttccc tcagacgcaa agctgaatcg ctcaggtcga
     1081 atcgccatcc gaaccttatc accttttttc aaaccatcca tttttgctgc ggacgaaaat
     1141 tgcacgccat ccagactaag caatcctttg gatggatcaa tcacttcggc agccgcagta
     1201 tttagcgaac ccacaaaatt tgcgacgaag gtggtctgtg ggaaattata aatttcaaac
     1261 ggtgtgccaa cctgctccac ttttccgtca tacatcacca caatgcggtc agaaatggat
     1321 aaggcttctt cctggtcatg tgtcacgaaa atggcggtga tccctaatcg tttttggatc
     1381 tcacgaattt gatcccgcag gctcacccga atctttgcat ccagcgccga gagaggttca
     1441 tccaaaagaa gcacgtgcgg gcgaatggcc agcgcgcgcg ctaaagagac acgctgttgt
     1501 tgtccgccag aaagctgata aggatattta tccttgtgtt tttccagatt taccagactg
     1561 agcatttcat tcactcgatc tgcaatatcg gccttgcttg cctttcgcac ccgcaatccg
     1621 aatccgatat tttgagagac ggtcatattc ggaaacagag cataggattg aaagatcatt
     1681 cccatgtttc gctggttggg cgccttttgt gttatgtctg ccccatccag cgaaatggtg
     1741 ccatctgttg ggctttcaaa ccccgcaacc atacgaagtg tcgtcgtctt cccacaacca
     1801 gacggaccaa gaaaagagac aaattcaccc ttttgaatat caaggttgaa gttatcgacg
     1861 acaacgtttt ctccgaactg tttggacacg ccattcaggc ttagaaatcc ctcgctcatt
     1921 actaattcct ctctcgtgaa atgtacgcga tcaaccccat tgccaaccag gtaatggcga
     1981 acgccacaat cgtcatcgcg gcgggctcat atactttgct acgtgtcaga tctgccatat
     2041 acggtcccaa tgtacgctga gccatgaata acgcaatggt caactcgccg atcacaatgg
     2101 caaaagtaag caatgcgccc gagagcaggg ccacgcgcag gttgggaaag atcacctgaa
     2161 gcaggattgt tgaccaattc gcgcccaggc tctgtgccgc ttccgtcagc gacctcacat
     2221 ccatcgcacg cagcccatta tcaaccgagc ggaacatgta cggaaaggat agtgccgcgt
     2281 aaacgcagat caggagtatt cgagaactgt cgtaggtggc cgtccaaaga agtggaggcc
     2341 agctgtacaa tcgtatgagc ccaaacacca gcaccactgc agggatgacg aacggcaata
     2401 gcgtaatgaa ttcaatgacg ggtcgcagtt tcggcaatcg cagtgtgacc caataggccg
     2461 tcggcacaat gatgagcaga ctgatgaaaa tcgctgcgac tgcgttcatg atcgattggt
     2521 tgaacgaagg caggaagttg gaatctgcaa acagcctgcg atatgcttcc agtccaagct
     2581 tgtccttctc cgctcgaagc gaccaataaa aggtggttgc cagtgggact aagaagtata
     2641 gggcagtcac gagataaatc agccagggac cgatcgcaga ttttttcact acaggttgat
     2701 ctgaagaagt ggcggttgat atcttcaggg gtgtagtcgg aatgctcatt tcagccacct
     2761 ttcagacctg cgctgaagtg gcaggtagat gaaaaagatg gcaaaagcca taatgaacac
     2821 catacccatg gcaattgcat gacctagccc ggggttgttc aggacatcac cagagatttg
     2881 ctgacctatc aacagagtag ctagcgtgaa aagtccaccg gtcaaggcgt acgctgtggc
     2941 atgcgcaccg aaggcatttc cgaaaagcaa aatcatggtg cccaggatcg atggtgtcaa
     3001 gacaggaaac gccacatcgc gccagtactg cgccgggctt gcccccagat tttcggctgc
     3061 ctcccgccac tcacgtttga gcccatcgat tgcagggagg atcgtgagta ccatcaacgg
     3121 gatttggaag tacaaataaa ccagggtaag ccctaaaaag gaatacaggc tgaatccact
     3181 gccatacaaa tcaaaaccta aaaattcaaa aaaggtggta atcaacccga ctcggcccag
     3241 ggtaaagaca aatgctgatg ccaggggaac cccagcaaag ttagcagcaa cgccggagaa
     3301 cgttgaagta agtgacctca gcggttttgg aagccctcca atggaaattg cataggcaag
     3361 gaggaaaccg agaaggcctc cgagaatcgc tgttgtgagg cttatcccga tagagatgcg
     3421 ataggcattg actgcgtttt gatccaacag ctgcccataa tactgaagtg ttaatccgcc
     3481 ctccggattt tcgatgcttc ggtacgcaag cagcaccgtc ggcgctatca ggaacaggcc
     3541 cgaaaataca aagaagggaa tgacaccgag ccattgccca gcatttaatc gaggggggcg
     3601 cttggcgccc ccctgcgttg atcttactgg tttaggatca gtgtttgctg tggtgcccat
     3661 gacaagttag ggcaagggct taacttcgac accgaccaca ctcggccaac cttccgcgat
     3721 gatcgcacgt gcggcggtga tgtcatcaac ggttgggaag taagcacctt cgatgcttgg
     3781 cagctgtgcg aagacttcat cagggatcac gcccgctgca ttcatggcat cgagccggat
     3841 cgggtaacag aaaccagagg cgtagataag ctgaccctca tccgagtaga ggtactccat
     3901 ccacaatttg gcggcgttgg ggtgcggagc ataggcactg attccctgta catacacacc
     3961 agcgatgatg ccttgcttcg gaacgttcgt gaccaggtca gggttgccag cgagggcaat
     4021 gtcgttggcg attgagttgt atgaccagtc gatcaggatt ggagtttcgc caataccgac
     4081 ggtgcgtgag tcgccatcga tcggaacaaa gttaccggct tcgttcagct ggcggaagaa
     4141 gtcgagcccg gcattggcag catccggacc agtagcacca gttgccagcc cggcagccca
     4201 aaccgataag gcagctgcgt tggaggtaag aggatcaccc gaaagagcaa cctgtccatt
     4261 gtactcaggc ttgagcaggt cagcatattc cgtgggaggg ttggcaactt cggcggtgtt
     4321 gacttccaat gaaagaacac cataatagtc accataccaa tagccattcg gatccttggc
     4381 accctctgga atatcttccc aggttgacac tttgtaggcc tgaagtaagc cctcttccac
     4441 cgcgtcaggg gcgagaccga taccaatatc tacaacatcc ggagcctgcg gaccggggtt
     4501 gtccttatta gctcgaatag cctccaactc ttcagccgaa ccggcgccag ggtcgagagg
     4561 attaatctcg ataccagggt atttggcttt gaacccttca tacatagcac catagttgca
     4621 ccagtcaaga ggaagggcga tcgtggtgag cattccttca gccttggctg cctcatagag
     4681 cgcattgtct gaagtggtag gtgcctcggt agctgcaggt gcacaggcag caagggcgag
     4741 gctcagggca atcagcaacg agaacagttg gaacaaatac tttctggtct tcattctctt
     4801 ctcctttgaa ttaagtactt gcggagagca cacgccctcc atcactgcga ttatatactg
     4861 aacaatacaa atgatggtaa tctttcgcga aaacttcgtt aacgagcgac ttcttccacc
     4921 tcttcatacc aacgaacacg tgtgcccacc ttatctcgca tctgccaggc caaagacttg
     4981 ggcatcgttt caaaggtttg ttgaatttct ttgactcgtt gttgaattaa acttcgttcg
     5041 gaatcttcca gcttaacatt ttcatcgcgt acgatttctt cgacgcgttt caggttgatg
     5101 gaagtggtct tatacagtcc ccaatcagcg ccacataatt ttgcgatctg cgataggttg
     5161 attttgctat catcatggcc ggttgcgtta aagagcaata atgaggcaag atcaagcccg
     5221 tctttgcgat tgagttcggc aatttgagct tttgtcagca atagctccgc caacggaatc
     5281 gtaaacggat gcacgtgcaa tcggtcttcc agcgggattt tatgacacat ctcaaaagag
     5341 ccaacgaaaa tatcgatctt catctcggtc tcgttgtgat agtaaatttg ccgctgcgca
     5401 ccattcaaca cattaaattg cttatgaggg gaataacctg ctgagggcat gaatgcctca
     5461 aattcgcggc gctgcttacc cgccacgata aaatccaggt cgccatactc acggcgaaac
     5521 gttggatgat cctctttttg ataaagcttt acacctaacc cgccaagcac gcgtatcaat
     5581 atcgattttt cgcgcgcggt tgagatcagg cgattcattt cgttttgaat atcaggtaca
     5641 atcgccataa ggattatctc attttaccgc tggtctgtcg catatgccat atgcgtaagt
     5701 aatcttttgg gaaggatcat cacaatgaac aaatcgctga gagatttgat gcacaaaggc
     5761 ctgatcactt gcccgcccga gacatcgctt ggaaatgtgg caatgctcct tacacaacat
     5821 catattcacg ctttagtggt tgttgagaat aacaaaccgc tgggaatcat ctccgatttt
     5881 gatctactgg ctggggagtg gctatccgta gatgcggaaa gcctcgacac aatgcgtcgc
     5941 atgaccgcgc gtgacctgat gacctatccc atcgctacga tcgagtctaa catttcggcc
     6001 gaagccgctg cgcaacagat gatcgaaaaa caagtacatc gaatgatcgt gatcgagaat
     6061 ggcgagcctg cgggcgtgat ttcgatttcc aattttgtgg ccagcatagc cgaacaggaa
     6121 cccgtgaaac gccagaccgt aggcgacgtt atgtctgaaa cattccttgt ctgtcgcgaa
     6181 gaaacctccg tgctctctgc cgcgcgtacc atgactcagg ctggctggcg ctccattgtg
     6241 gtcgtgaatg cgacaggcaa gttacttgga ttcattaccg gcagggatct gatgcatctg
     6301 gccgggcgaa acgtcgatga gaaattgaaa gtcagccaat gcatgaatcg gagtctgctc
     6361 actgtagata tcaacgccag cttgcaggaa gccgccgacc tgatgattca aaaccatcgc
     6421 catcgtgtag tggtcatcga caccaacgac cccgatagtt ttccacttgg ggtgctctct
     6481 tcatttgata tcgttgctga gatggcaaga cccggatcga tatggcagag ttaaaatccc
     6541 ggcacagaat aactgatgat ttgggagtga atttagtaga tatttgcgct ctgtaaaact
     6601 aagattccag gccggaataa aagagacagg caacctgcaa tacaagcctg tctctttttg
     6661 tatttatcaa agtagatttt tattctactg ggatctctgc ttgaacctta tccaggtcaa
     6721 ttccttccgt cttgcgacga gctttggagc cgtagtagat ggcagccgcc aaaccgtaac
     6781 aggctgccag gaaaatgatg gagtttgtat ttttaaagct gatgccatac agacctgcac
     6841 cctctccgcc tgtattgagc caggggtcaa acatccattc aacaat
//
LOCUS       DASTJP010000724         4240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_440699, whole genome shotgun sequence.
ACCESSION   DASTJP010000724 DASTJP010000000
VERSION     DASTJP010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440699"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1652)
                     /gene="xylB"
                     /locus_tag="VFQ23_21175"
     CDS             complement(165..1652)
                     /gene="xylB"
                     /locus_tag="VFQ23_21175"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET9909175.1"
                     /translation="MAYFLGIDTSTTSSKALLIDEQGEVIAVASSPHTLQTPRALWSE
                     QDPREWWDAVTVSIKSVLEKAGIGGERIAAVGLTGQMHGLVLMDEAGNVLRPAILWND
                     QRTQSQCDEIHHIIGKEKFIQITGNVALTGFTAPKILWVKENEPEVFAKARHVLLPKD
                     YIRYKLTGEYAMDKADGAGTVLFDLKARDWSEKVLSALDIPRLWMPKTFEGPEFTGYI
                     TREAELLTGLKAGTPVAAGGGDQAAQAVGVGAVEPGIVGLTVGTSGVVFATTPSALIE
                     PEGRLHAFCHAVPGMWHFMGVMLSAAGSLQWFRDTLAPNVSFDDLLKEAESVPAGSEG
                     LQFLPYLSGERTPHPDPLARGAFIGLTLRHGRAHMTRAVLEGVAFGLKDSFTLIQNAG
                     LGAITQVRASGGGTKGALWRQILASVLDAELVTVNTTEGAAYGSALLAAVGAGAWNDV
                     PYACKMSVKITGGTLPDPAQVEVYQTAYSLYQELYPALKPSFSQM"
     gene            complement(1666..2832)
                     /gene="xylA"
                     /locus_tag="VFQ23_21180"
     CDS             complement(1666..2832)
                     /gene="xylA"
                     /locus_tag="VFQ23_21180"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HET9909176.1"
                     /translation="MADFTPKPENKFTFGLWTVGNRGADPFGYAVREGKSPAELVRLL
                     GEVGAWGVNFHDNDLIPIDATASERDAILKDFKQALADTGVVVPMATTDLFREPIFKD
                     GAFTSNDSKVRAYALQKTMNAIDLGVELGAKIYVFWGGREGTETDAAKDPLTSIKRSR
                     EAMNFLCEYVKDKNYDLRFALEAKPNEPRGDIYNSTTGHMLAFIETLDHPEMVGVNPE
                     VAHEHMAGLNFVHGVAQALEAGKLFHIDLNDQAFGRYDQDFRFGAVNIKSAFFLVKLL
                     EDNTYDSSRHFDAHAYRTEDYEGVKDFARGCMRTYLILKEKAAQWNADQEIQSLVAEI
                     NADDGSMNQYFGTYSAEKAKALKAQAFDRNAIATRGLQYERLDQLTVEVLLGVR"
     gene            complement(2891..>4240)
                     /locus_tag="VFQ23_21185"
     CDS             complement(2891..>4240)
                     /locus_tag="VFQ23_21185"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET9909177.1"
                     /translation="KVTKDAAESAALALEHGCDLGCDHVFDEIPEAIRRGLITEADVD
                     RSLERTLATRLKLGMFDAPEDVPFASISMDVVACDEHRQLAYRTATESVVLLKNKNNI
                     LPIKPSTKKIFVTGPTATSMEVLLGNYYGFNNQMITLLEGITGRVPEGMGMEYTSGAM
                     LKHPREIKSTWAPGMAQSADFAIVCAGFSSFLEGEEGESLLSPPNGDRDSISLPDSQV
                     NYIKELAIHGVKIVLVLTGGSPIALGEVEEMVDAILFVWYPGMEGGRAVANVLFGDVS
                     PAGKLPVTFPKSLDQIPAFDDYSMNGRTYRYMTEEPLYPFGFGLSYNRFDYSDLQLDS
                     TTLTSGDSLNVSLTLTNCGDSDSAEVAQFYVSDLHASTTVPLHHLVGFERVDLKAGES
                     KALKFTITPEMMSFYNDDGKLTLEPGEFRLEVGGCSPGRRGQELGAPKPVNAVFEIK"
BASE COUNT          993 a         1174 c         1030 g         1043 t
ORIGIN      
        1 cgcaccgaat ggcgcgcccg agcatggatt attgcaagga aggtcggctt ctctttttcc
       61 ctgaggcgac ccattgggtt caacgcgatg aggctgagga agtaaacagg cacctgctcg
      121 atttcgtatt tatgtaagcc gcccgtatcc atcgtcgttc ttcatcacat ctgtgaaaaa
      181 ctgggcttca atgccgggta taactcctgg tagagcgaat atgccgtctg atacacctcg
      241 acctgtgcgg gatctggcaa tgtacccccc gttattttga cggacatttt gcaggcatat
      301 gggacgtcat tccacgcacc ggcgcccacc gctgccagca acgccgaccc atatgccgcg
      361 ccttctgttg tattaaccgt gaccaactcc gcatccaata cggaagcaag gatttgcctc
      421 catagcgcgc cctttgttcc accaccggag gcgcggactt gtgtgatggc gcccaaacct
      481 gcattttgaa tcaaggtaaa gctgtccttc aacccaaaag ctactccttc gagtacggca
      541 cgcgtcatgt gcgcgcggcc gtggcggaga gtcagcccga tgaacgcacc gcgcgctaac
      601 gggtcagggt gaggggtgcg ctctccactc aggtatggaa ggaattgcaa gccttcactt
      661 cccgcaggaa ccgactcggc ttccttcaat aaatcatcga aacttacatt cggcgcgaga
      721 gtatcccgaa accactgcag gctacccgcc gcagaaagca tcacccccat gaaatgccac
      781 atgcctggca ccgcgtgaca aaatgcatgt aagcgccctt ccggttcgat caatgcagat
      841 ggagtcgtgg caaacacaac gccacttgtg ccgacggtca gcccaacaat gccaggttct
      901 actgcaccca caccaaccgc ttgcgcagcc tggtcacctc cgcccgccgc gaccggcgta
      961 cccgctttta gtcctgtgag taactcggct tctcgcgtta tgtatcctgt gaactccggt
     1021 ccttcaaagg tttttggcat ccacaggcgt ggaatatcga gtgccgataa aactttctct
     1081 gaccagtccc gcgcctttag atcaaacagc accgtgcccg ccccatcggc tttatccatt
     1141 gcatactcgc ctgttagctt gtagcggatg taatccttgg gcagtaaaac gtgcctggct
     1201 tttgcaaaca cctccggttc attttctttt acccataata ttttcggcgc ggtaaatcca
     1261 gtcagcgcga cgttacctgt gatttgaata aatttttctt tgccaatgat gtgatgaatt
     1321 tcatcacatt gactctgcgt gcgctggtca ttccataaga tcgccggacg caaaacgttt
     1381 ccagcttcat ccatcaaaac cagaccatgc atttgccccg tcaggccaac agctgcaatt
     1441 ctttccccgc cgatgcctgc tttttccaat actgacttga tactcactgt gactgcgtcc
     1501 caccactcgc gcggatcctg ctccgaccac aaggcacgcg gcgtctgcag cgtatgcggc
     1561 gatgaggcta ctgctatcac ctcaccctgt tcatcaatca acagtgcttt gctggaagtt
     1621 gtggaggtgt cgatgccaag aaaataagcc atagtggttg tgattctagc gtacgcccaa
     1681 caagacttcc acagtcaact gatcgagtcg ttcgtattgc aagccacgag tcgcaatagc
     1741 attgcgatca aaggcttgcg ctttgagtgc ctttgccttt tcagcagaat acgtgccgaa
     1801 gtattggttc atcgagccat cgtccgcgtt gatctcagcg acgagtgatt gtatttcctg
     1861 atctgcgttc cattgagcgg ctttttcctt gaggataaga tacgtgcgca tacagccgcg
     1921 tgcaaaatct tttacacctt cgtaatcttc agtacgatag gcatgcgcgt caaaatggcg
     1981 tgagctatca tacgtattat cttccagtaa cttaacaagg aagaaggcac tcttgatatt
     2041 caccgcgcca aagcgaaaat cctgatcata acgaccaaag gcctggtcat tcaaatcaat
     2101 gtggaataac ttacctgctt ccagggcttg tgctacgccg tggacaaagt taagcccggc
     2161 catatgttca tgcgccactt cggggttgac acccaccatt tcaggatgat ccagcgtttc
     2221 aatgaatgcc agcatatgac ccgttgtgga gttgtaaata tcgccgcgtg gttcattcgg
     2281 tttcgcttcg agcgcgaatc tcaaatcgta attcttatcc ttcacgtatt cacataaaaa
     2341 gttcatcgct tcacgtgaac gtttgatgga tgtgagcgga tccttcgctg cgtcggtctc
     2401 agttccctca cgcccacccc agaagacgta gatcttcgcg ccgagttcga ctccgaggtc
     2461 aatcgcgttc atcgtctttt gcaatgcata tgcgcggacc ttggaatcgt tcgatgtaaa
     2521 tgcgccatct ttgaatatcg gttctctgaa tagatccgtt gttgccatcg gcacgacaac
     2581 gcccgtatct gcgagcgctt gcttgaaatc tttcagaatg gcatctcgtt cggatgcagt
     2641 tgcatcgatg ggaatcagat cgttatcgtg gaagttgact ccccacgcgc cgacttcgcc
     2701 cagtagacgg actaattcag caggtgattt gccttcgcgc acagcgtacc caaatgggtc
     2761 cgcaccacgg tttccaacgg tccatagacc aaaggtgaat ttgttttcag gcttgggggt
     2821 gaagtctgcc atggaatact ccttttagaa aacctgtcag gtttttgaga actgacaggt
     2881 ttataaaaat ttactttatt tcaaacaccg cattgactgg ctttggcgcg cccaactcct
     2941 gaccgcgtcg gccgggagag catccgccta cttccagccg aaactcaccc ggttcgagag
     3001 taagctttcc gtcatcgttg tagaacgaca tcatctcggg agtaattgtg aatttcaaag
     3061 ctttactttc acctgctttg agatctacgc gctcaaagcc cacaagatga tgcaacggga
     3121 cagtagttga agcgtggaga tcgctcacgt aaaactgggc cacttcggct gagtctgaat
     3181 cgccacagtt ggttagggtc aggctcacgt tgagggaatc gccagaggta agagtggtgc
     3241 tatcgagttg cagatccgaa taatcaaaac ggttataact cagaccgaaa ccaaagggat
     3301 aaagcggctc ctcggtcata tagcgatagg tgcgtccgtt catgctgtaa tcgtcgaagg
     3361 cagggatttg atcgagtgat ttggggaagg tgaccggaag tttgccagca ggagagacat
     3421 ctccaaatag aacattagct acggcacgac cgccctccat gccgggatac caaacgaaga
     3481 ggattgcgtc taccatttcc tccacttcac ccagcgcaat cgggctgccg cccgtcagca
     3541 ccagcacgat ctttacaccg tggatcgcga gctctttgat gtagttcacc tgactatcag
     3601 gcagagaaat gctatcgcga tcaccgttgg gaggcgagag caacgactcg ccctcttcgc
     3661 cctcgagaaa cgaagaaaag cccgcacaga caatcgcaaa gtctgcggat tgtgccatcc
     3721 cgggtgccca cgtactcttg atctcacggg ggtgtttcaa cattgctcca gaggtgtatt
     3781 ccattcccat tccctcggga acgcgccctg tgatgccttc cagtagagtg atcatctggt
     3841 tgttgaagcc ataatagtta ccgagcaaca cttccatgct tgtggcagtg ggacccgtga
     3901 caaatatctt ttttgtggag ggtttgatcg gaaggatatt attcttattt ttcaacagca
     3961 ccactgattc ggtcgcggtg cgataggcca gttgacgatg ttcatcgcaa gccacaacat
     4021 ccatcgaaat agaggcaaag ggaacatctt ctggcgcatc gaacatgccc agcttaagtc
     4081 gtgtggctag cgtacgctcc agtgagcgat cgacgtctgc ctcggtgata aggccacgcc
     4141 ggatcgcctc gggaatctca tcaaaaacgt gatcacaccc caggtcgcat ccatgttcca
     4201 aagccagtgc agcggattca gccgcgtcct tcgtgacctt
//
LOCUS       DASTJP010000725         5762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_591208, whole genome shotgun sequence.
ACCESSION   DASTJP010000725 DASTJP010000000
VERSION     DASTJP010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591208"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFQ23_21190"
     CDS             complement(<1..401)
                     /locus_tag="VFQ23_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HET9909178.1"
                     /translation="MNALATYYYPNRMGRIILLSMEEVMGRNGVNALLKLASLHSLIE
                     NYPLDNTQSAFPFSTVSNLTEMLEQAYGPHGGRGLALRIGRACFNYGVRQYSTQLGLT
                     EMAFRLLPLPTKLHAGASAFAELFNNFTDQRV"
     gene            complement(398..1255)
                     /locus_tag="VFQ23_21195"
     CDS             complement(398..1255)
                     /locus_tag="VFQ23_21195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9909179.1"
                     /translation="MSSITTDQGIVHYEVYGRGKPVILLHGWLGSWGLWQETMSFLGR
                     YYRTYALDFWGFGESGKKRETYAVQDFVGLVNQFMDQLGIVHAPLVGHSMGGTVSLSV
                     AIRFPERVSKVVVVGSPMVGSSLAPLLKLLGLRPVAFMLFNMMSVFRVGMKLYSPLIC
                     SDPRFPAMMDRDLTRTTLESFLLSIGSLRRTDLRPMLDQIKIPALGIYGDRDIIVHPK
                     QWQPMQKGIAHAQIQRLPLAGHFPMLEEPQPFAERLKAFLDKDDSLSSPQTSASLSPT
                     PSPSVTLSP"
     gene            complement(1284..1823)
                     /locus_tag="VFQ23_21200"
     CDS             complement(1284..1823)
                     /locus_tag="VFQ23_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HET9909180.1"
                     /translation="MQTVKMVVTGPFSAGKTEFIQSVSEIDVVSTERKISSMAERSVK
                     EATTVAMDFGRITVDEDLVLYLFGTPGQKRFDFMWEILSEGMLGFIVMVDSTRPETFR
                     EARSILETFRAYAPTPYVVAANKQDKEDAWDLEDMRHALRLDSKVKFLPCVATDKDTV
                     KSVLLELLYNILAEMESEG"
     gene            complement(1846..2652)
                     /locus_tag="VFQ23_21205"
     CDS             complement(1846..2652)
                     /locus_tag="VFQ23_21205"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9909181.1"
                     /translation="MALRGNLRDFTITQLLNLINLAQKTGTLVVDGPSEQAYVSFRDG
                     KLAYARVGQDDGSLAAVLHKANKLTVNQYRAIVERAGKITDKELGLLLINAGYLSQEE
                     ILANLQGYFTEAIRRLFTWVEGFFRFEGEMLPPDDRINVRLDLENIIIEGSRQLREWE
                     QLQDEIPSLEMALKFTDRPLKNVNLSVEEWRVVSFINPKNTMKQIAQTNKMNDMEVRR
                     IVYGLLQAGLVEIIRPEGMVIQQSPKTFPTQNKEEQKSLVNKLIGRIRTL"
     gene            2817..4187
                     /locus_tag="VFQ23_21210"
     CDS             2817..4187
                     /locus_tag="VFQ23_21210"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HET9909182.1"
                     /translation="MYRTILALILNFSLVFVTSAFTLPRLQITNDQVILDFPISATFR
                     ASFTSDSEIQSIILEYGNEQQNCGEVIAKAFPQFTPGRTVKAEWTWEMRQSGSLPPGA
                     TLWWRWRITDANGNETVSERKTATWLDDIHNWQSLNNGDFIRLHWYEGDQNFAIDLAK
                     AANDGLQFNETNSGLKAESPIDIYIYADTNDLKDAILYEPSWAGGQAFPNQDIVILGI
                     SQSDLDWGRDAIVHELTHVLVGHLTFSCLGDVPTWLNEGLAVYSEGELERSSQRQLEN
                     AIREDTLLTVRSLSSGFSEVSDKAYLSYSQSYSLTKFLIETHGQEKMTLLLVSLRDGL
                     AIDEALTQIYGFNIDGLEAAWRQDIGAAPSAPLEPASMRPTPTFVPTIVPLSIAPVVN
                     QSTPTAIPTSTFDEPTETPVRTAPPLWLTLTLLGFCCVFLLLIGIVVLGFVVRRQNQA
                     GGENAR"
     gene            4177..>5762
                     /locus_tag="VFQ23_21215"
     CDS             4177..>5762
                     /locus_tag="VFQ23_21215"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9909183.1"
                     /translation="MLDRAHREANSTMKTNISKILLLTLSVGITLGLASPKAAGAWQS
                     DIPHNQALVLEGGESTNPREFDPATTHSSGDKRIFSGLVSFDPQLNLTPDLAERWEIS
                     DDRMTYTFHLRANAKFHNGRAVTAQDVIYSWERAASPSLASDTVSTYLGDILGVNEMI
                     SGEVNSIEGLLAVDEHTLQVRIDAPKPYFLLKLTFPTAFVVDKENVESSEEWYRQPNG
                     TGPYRLTEWVSFERKIYEANEEFYLGAPSIPYILVNLYSGDSQALYETDDVDLTHVYS
                     IERFTDPTEPLHQELRSGVSLCTGYIVFDTTQPPFDDVNVRKAFSMAFNRQQYIDVVL
                     DGHALPARGLYPPGLPGFNLSLQGLSYDPAQARQLLEQSKYGGANGLPPIVFTDSGIG
                     SSIGGSIAAVAQMWKQDLGVTVTVENLEPSFYYDRIHAGQHGQLLDGGWCADYPDPEN
                     FADVLFHTASTQNSGGYSNPDLDSLLEAARVEQDVTKRMAMYQQAEQILVDDAAALFT
                     VHWLSYELVKPYVQGYVLTP"
BASE COUNT         1500 a         1413 c         1384 g         1465 t
ORIGIN      
        1 acccgctgat ctgtaaaatt attgaataac tcagcaaaag cgcttgcccc agcatgtaat
       61 tttgtaggga gcggcaatag tcgaaatgcc atctcagtta accccaattg tgtgctgtat
      121 tggcgtactc cataattgaa gcaggcacgt ccaatgcgga gagcaagccc gcgccctcca
      181 tgtggaccat aagcctgttc gagcatttcc gtgagattgc ttacggttga aaatggaaac
      241 gcggactgcg tgttatccag cgggtaattc tcaataagag agtgcagcga ggcaagtttt
      301 aagagggcgt tgactccgtt acgccccatt acttcttcca ttgacaacag gataatacgc
      361 cccatccgat tcggataata gtatgtggca agagcattca tggggataga gtcaccgaag
      421 gggaaggagt tggggatagt gacgcggagg tctgtggcga ggaaagacta tcatccttgt
      481 ccagaaaggc tttcagtcgt tctgcaaagg gttgcggttc ttccagcata gggaaatgtc
      541 cggctaatgg aagccgctgt atctgagcat gagcgattcc cttttgcatg ggttgccact
      601 gttttgggtg gacaatgatg tcacgatcac cgtagatgcc taatgcgggg attttgatct
      661 gatccagcat cgggcgcaaa tctgttcggc gcagagagcc gatggaaagt aagaacgatt
      721 ccagggtcgt acgcgtcagg tcgcgatcca tcatcgcagg gaaacgcgga tcgctgcaga
      781 ttagcggaga atataacttc atgccgacgc ggaaaacact catcatgttg aatagcataa
      841 aagccaccgg gcgaaggccg aggagcttaa gcagcggtgc cagcgatgag ccaaccatgg
      901 gcgagccgac aacaactact ttgctgacac gctcagggaa acggatcgct accgaaaggc
      961 ttacggtacc acccatgcta tgaccgacaa gcggcgcgtg cacgattccc agttggtcca
     1021 tgaactgatt gacgagaccg acaaaatcct gaactgcata ggtttcacgt ttcttgcccg
     1081 actcgccaaa cccccaaaaa tccagagcat acgtccgata ataacgaccc agaaaactca
     1141 tggtctcctg ccacaacccc caggacccaa gccaaccatg caaaagaatg acaggtttcc
     1201 cacgcccata aacttcgtaa tgaacgatgc cttgatcagt agtaattgat gacacgactt
     1261 cttaaactcg cggcaggctc cccttatcct tccgattcca tttcagcgag aatgttatag
     1321 agtaattcca agagcacaga tttcacggtg tctttatcgg ttgcaacaca aggaaggaac
     1381 ttaaccttgc tatcgagccg caaggcatgc cgcatatctt cgagatccca ggcgtcttct
     1441 ttatcctgct tgttcgccgc aacgacatag ggagttggtg cataggcacg gaaggtctca
     1501 agaatggagc gcgcctcccg aaacgtttca gggcgggtgc tgtcaaccat cacaataaat
     1561 cccagcatgc cctccgaaag gatctcccac atgaaatcga atcgtttttg accgggcgtg
     1621 ccgaatagat aaaggacaag gtcttcgtcc acagtgatgc gaccgaaatc catcgcgacc
     1681 gttgtcgctt ccttaacgga acgttcggcc atgctggata tctttctttc agtggagaca
     1741 acatcaattt cgctgacaga ctgaataaac tccgtcttgc cggcgctaaa ggggcctgtg
     1801 accaccattt ttactgtttg cataatctgt gtccttccac ctgggttaaa gagtacgaat
     1861 gcgtccaatc aatttattaa ccaacgattt ctgttcctct ttattttgtg tcgggaacgt
     1921 cttggggctt tgttggatga ccatgccttc aggtcggata atttcgacaa gcccggcctg
     1981 taataaacca taaacaattc ggcggacttc catatcgttc atcttattcg tttgcgcaat
     2041 ttgcttcatt gtattcttag ggttgataaa tgaaacaacg cgccattctt ccacactgag
     2101 atttacgttc ttgagcggac gatccgtaaa tttgagggcc atctcgaggc tgggaatttc
     2161 atcttgtaat tgttcccatt cacgcaactg acgtgagcct tcgataataa tattttccag
     2221 gtcaagccgc acattgatac ggtcatccgg cggcagcatt tcgccttcaa aacgaaagaa
     2281 accttccacc caggtaaaga ggcgtcgaat cgcttccgtg aaatacccct gaagattggc
     2341 aaggatttct tcctgtgaaa gataaccggc attgatgagc agcagtccca actctttatc
     2401 ggtaatcttc ccagctcgtt ccacaatcgc acgatattga ttgaccgtta gcttatttgc
     2461 tttatgtagc actgccgcca ggctgccatc atcctgccca actcgtgcat aagcgagttt
     2521 tccatcacga aaggaaacat aggcctgttc actgggcccg tcgacgacca gcgtaccggt
     2581 cttctgggcc aaattaatta aattaagcaa ttgtgtaatg gtgaaatcac gtagattgcc
     2641 gcgcagggcc atatatacct caacaaaatc tccacgatgg gagactcaaa agattataca
     2701 tgcaagggaa acatgcgtca aacgactgcc tcttgattga tatgtgattg gaaggtgaca
     2761 catttcttcg agaaatgaaa cactgataca cttataaaaa gattttttgg agttttatgt
     2821 atagaacaat cctagcactt atccttaatt tcagcctggt gttcgttaca tcagccttca
     2881 ccctacctcg actacaaatc acgaacgatc aggtaatctt ggactttccc atatcggcga
     2941 cttttcgagc ttcgttcacg agtgattcgg agatccaatc gatcatcctg gaatacggaa
     3001 atgagcaaca gaactgcggg gaggttattg ccaaggcttt tccgcaattt acacccggaa
     3061 ggaccgtcaa agcggaatgg acatgggaaa tgcgtcagag cggctccctg ccgccgggtg
     3121 caacactctg gtggcgctgg cgcattaccg atgcaaacgg aaatgagacc gtcagcgaaa
     3181 gaaagacagc tacctggctg gatgatatcc ataattggca aagcttgaac aacggcgatt
     3241 tcattcgcct gcactggtat gagggagacc aaaactttgc gattgacctg gcaaaagcgg
     3301 ctaacgatgg tttgcagttt aacgaaacaa attccggact aaaagcggag tccccgatag
     3361 atatttatat ttatgcggat acaaatgatt tgaaagacgc cattctttat gaaccatcct
     3421 gggctggtgg gcaggcattt ccaaaccagg atatcgtgat tctgggaatt tcacaaagcg
     3481 acctcgattg gggacgcgat gctatcgtcc atgagcttac acacgtcctt gtcgggcatc
     3541 tgactttttc ctgtctcggc gacgtaccta cctggctcaa tgaaggtctg gccgtttaca
     3601 gcgaagggga acttgagcgc tcctctcagc gacaactaga gaatgccatc cgcgaggata
     3661 cattgttaac tgttcgctcg ttgagttctg gtttttccga agtctccgac aaggcatatc
     3721 tctcctacag tcagtcttat agcctcacaa aattcctgat cgaaactcat ggtcaggaaa
     3781 aaatgacatt gctgctcgtt tcattgagag acggtttggc aattgatgaa gccctcacac
     3841 aaatctacgg attcaatatc gatggtcttg aggcggcatg gagacaagac atcggcgcgg
     3901 caccgtccgc tccgttggaa cccgcctcca tgcgtcccac gccaaccttc gtacccacaa
     3961 tcgttccact ttctattgct ccggtcgtta atcaatccac tccaacggca atacccacgt
     4021 caacttttga cgagccgact gaaaccccgg tacgtaccgc tccgcctctt tggctgacac
     4081 ttacattgct gggtttttgt tgcgtttttc tattgctgat tggtatcgtg gttctgggat
     4141 tcgtcgttcg caggcagaat caagctggag gggaaaatgc tcgatagagc acatcgcgag
     4201 gcgaacagca ccatgaaaac taatatttca aaaatacttt tattgacatt atctgtcggt
     4261 attacacttg ggctggcttc accaaaagca gcaggggcat ggcaatcgga tattccgcat
     4321 aatcaggcgt tggtcttgga gggtggtgag tctactaatc cgcgcgagtt tgatccggcg
     4381 acaacccata gttccggaga caaacgaatc ttcagtggat tggtctcttt tgatcctcaa
     4441 ctgaatctca cccccgatct cgccgagcga tgggaaatca gcgatgatcg gatgacgtac
     4501 acctttcatc ttcgcgcgaa tgcgaaattc cacaatggac gcgcggtcac ggctcaagat
     4561 gttatctatt catgggaaag agctgcaagc ccaagcctgg cgtcagacac agtatccact
     4621 tatctgggtg acattttggg cgtgaatgaa atgatatctg gggaggtcaa ttcgattgag
     4681 ggacttctcg cagtggacga gcatacctta caagtgagga tcgacgcgcc aaaaccctac
     4741 ttcctattga agctcacttt tcctacagcc tttgtggtgg ataaggaaaa cgttgagagc
     4801 agtgaagaat ggtaccgcca accaaatgga actggtccct atcgattaac cgaatgggtt
     4861 agttttgagc gaaaaattta cgaggcgaac gaagagtttt atttgggagc accgtccatt
     4921 ccctatattc tcgtcaattt gtattctggg gatagtcagg cgttgtatga aaccgacgat
     4981 gtggatctga cccatgtata ttccattgaa cgcttcaccg atcccacgga gccacttcat
     5041 caggagttgc gttctggagt ttccctttgc acaggataca tcgtatttga tacaacacag
     5101 cctccattcg atgatgtgaa tgtgcgcaag gctttttcaa tggcattcaa tcgccagcag
     5161 tatattgacg ttgtgttgga tgggcacgcc ctacccgctc gtggactcta cccgcctggc
     5221 ttacctggtt tcaacctttc actgcaggga ttatcgtatg atcctgcaca agcacgccaa
     5281 ttgttggaac aatctaaata cggtggagca aatggactgc cgccgattgt atttactgat
     5341 tcaggcatcg gaagttcgat aggcgggagc attgcagccg tggctcaaat gtggaaacaa
     5401 gatttaggcg tcactgtcac tgtagaaaat ctggaaccga gtttttacta cgatcgaata
     5461 catgcgggtc agcacggtca attgctggat ggcggctggt gtgcagatta tcccgaccct
     5521 gaaaatttcg cagatgttct gttccatact gcttctactc aaaatagcgg tggttattcc
     5581 aatcctgatt tagactcact tcttgaagcg gcgcgtgtcg aacaggatgt gaccaagcgt
     5641 atggcaatgt atcaacaagc tgaacaaatt cttgtagatg acgctgctgc gctttttacc
     5701 gttcattggc tctcctatga actggtaaaa ccttatgtgc aaggatatgt ccttacgccg
     5761 at
//
LOCUS       DASTJP010000726         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_485945, whole genome shotgun sequence.
ACCESSION   DASTJP010000726 DASTJP010000000
VERSION     DASTJP010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485945"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFQ23_21220"
     CDS             complement(<1..737)
                     /locus_tag="VFQ23_21220"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET9909184.1"
                     /translation="MNNRIQYIDIARGIGILLVVLGHNDFALVSPFAYQVIYSFHMPL
                     FFFLSGYFINPSAGFWEFVRRRFNSLLKPYLFTIFMIYFVSISFEKMSFQTALFRITK
                     SLYGSGYYLDWVQLWFLPHLFVVSLYAYLFLNLFNRLNNHWVRWGILLATLEISLLFL
                     KTFYPFPFQAFGNDYELYGLPFSLDLVLLSGFFFILGSEVRQITSEETFSSPILLFGT
                     GMALLLLNFFFPAKIDFNTRLYESFLI"
     gene            complement(809..1735)
                     /locus_tag="VFQ23_21225"
     CDS             complement(809..1735)
                     /locus_tag="VFQ23_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET9909185.1"
                     /translation="MLTIYKTTEQGLEQLESMANGTWVKVIDPTPEETQQLVNWGIDA
                     EYINYSLDLDEMPRIERDDDYTFILIRIPHRQPNDDIPYITIPLGIMIKGNTIVTICR
                     HDKEMFKVLANGKYRLLKTGKRYRFALYIFLETATRYLTHLREINRITEALEDQLQKS
                     TRNSEVLELLKYQKSLTYFATALRSNEVMMERVQRTQIFNYYEDDQDLLEDVLTENQQ
                     AIQMTSINTEILSSMMDAFASIISNNLNGVMKGLAAITIIINLPAVVAAFYGMNVQLP
                     GEGHPLAFLTVFAISLTLTALATFIFYKRDWF"
     gene            2031..3230
                     /locus_tag="VFQ23_21230"
     CDS             2031..3230
                     /locus_tag="VFQ23_21230"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9909186.1"
                     /translation="MLDTFVKLYFNAPDMIPGLDGLRALAFLLVFAFHTDYLQVGWVG
                     VSLFFVLSGFLITGILLDMKKSLSTRDYFFKFYGRRFLRIFPLYYFYLILMTIVTTWL
                     IGIVYRPLYMQIFLDQVRFAFFYVYDFFFGTANFQYSFFLDHFWSLSVEEQFYIFWPL
                     SVLLVPEKSLKKLFLGFIVLGPILRLAVYFIHQSGVFRFLADTAPLAIFPLPFSYIDA
                     FAFGAYISRFTIPKAKTQFFLLLGIMPIIGYGAQYLATGKIGTVSALGYPLLLPESYQ
                     FIWGYTLLNYFFAVTIYSVVNDRIFIRFLESPPLQYLGKISYGLYVYHFPIIWFSMRL
                     RDFGIVDPFAKPVIAVVSFVATLLVASISYKLLEQPLLNLKDRFFNRRETSVPEGVRL
                     PLKSDLS"
BASE COUNT          866 a          773 c          773 g          992 t
ORIGIN      
        1 ttgatcagaa atgattcata gagccttgta ttgaaatcaa tctttgccgg aaagaagaaa
       61 ttgagaagca gtagagccat gcctgttccg aacaaaagaa tcggactgct gaacgtttcc
      121 tcagacgtga tctgccggac ctcactgccc aaaatgaaga aaaagccgct taggaggaca
      181 aggtccaggc taaatggcaa accatacagc tcgtagtcat ttccgaaggc ttgaaaagga
      241 aacggataga atgtcttcaa aaacagaagg gaaatctcca gggtggcaag aagaatgccc
      301 catcgcaccc agtggttgtt cagccgattg aaaagattga gaaagagata agcatacagg
      361 cttaccacga acaaatgggg caggaaccag agctgcaccc aatccaagta atatccactg
      421 ccgtataaag acttggtgat tcggaacaaa gcggtctgaa aactcatctt ttcgaatgag
      481 atcgaaacaa aatagatcat gaagatcgta aaaagatacg gcttcaagag agaattgaaa
      541 cgccgtctca caaattccca aaagcccgcc gatgggttga tgaaataacc ggacaggaaa
      601 aagaacaacg gcatgtggaa tgaatagatg acctgatacg cgaaagggga taccagtgcg
      661 aaatcattat gccccaatac gaccagtagt attccgatgc ctcttgcaat atcgatatat
      721 tggattcgat tgttcaagga gatgattcct ctcctcaaat tttaccctgc cagctccaat
      781 cctggcatac gagaggaacc cgtggatctt agaaccaatc gcgtttatag aagatgaacg
      841 tagccagcgc agtgagggtg agtgaaattg cgaacacggt cagaaaagcg agcggatgac
      901 cctcgcccgg caattggaca ttcatcccat agaaggcggc gaccactgca gggagattaa
      961 tgatgatcgt gattgcagcc agacccttca tcacgccatt caggttgttc gaaataatcg
     1021 aggcaaaagc atccatcatg cttgagagaa tttctgtgtt gatgctggtc atctggatgg
     1081 cttgctgatt ttcagtgagc acatcttcca gcagatcctg atcgtcttca taatagttga
     1141 aaatctgtgt ccgctggacg cgttccatca tcacctcgtt ggagcgaagc gcagttgcaa
     1201 aatatgtgag gctcttctgg tatttgagta attccagcac ttcactattg cgtgttgatt
     1261 tttgcagttg atcttccaat gcctcagtaa tacgattgat ctcgcgcaag tgggtcagat
     1321 aacgagtggc agtctcgagg aaaatataca atgcaaaacg gtaacgcttg cctgttttga
     1381 gcaggcgata ttttccgttg gcaagcacct tgaacatttc cttgtcgtgg cggcaaatgg
     1441 tcacgatggt attgcctttg atcataattc ccagtggaat ggtgatataa gggatatcgt
     1501 cgttaggctg gcgatgagga atacgaatca ggatgaaggt gtaatcatca tcccgttcga
     1561 tacgaggcat ttcgtcaaga tccagtgaat aattaatgta ttcagcatca atcccccagt
     1621 ttacaagttg ttgagtttcc tcaggagtgg gatcgatcac ctttacccat gtgccgttcg
     1681 ccatggattc gagttgttcg agcccctgct ctgttgtttt ataaattgtg agcattactg
     1741 cctcctacac gtgaaattat cacgcaggag actagtccat ctccttcgtg atattagggg
     1801 tattttcttg caatgcgtgc cgcccgcgca gcgcgcggcc tactacttgg cggttgactt
     1861 tcggttgaac ctatatcgct ctcatcaaga agatacatca ttgatccttg ccacctgctc
     1921 gcgcagatgg cttgggtatt ttacgcctgt taagggaaat ccacaacgat tatgtggatt
     1981 gcaatttgga tacaacccgg cgatgttatc gtaattagca caaactcacg atgcttgaca
     2041 cctttgtgaa actgtatttc aatgcacccg atatgatccc cggactggac ggcctgcgcg
     2101 cccttgcctt tttactcgta tttgcatttc acaccgatta tctgcaggtt ggatgggtgg
     2161 gtgtatcttt attttttgtc ctgtcaggtt ttttgatcac gggcatcctg ctggatatga
     2221 aaaaatccct gtctaccagg gattattttt tcaaatttta tggcaggcgc tttcttcgaa
     2281 tatttccgct gtactatttt tatctcatcc tgatgacgat agtgacgacc tggctgatcg
     2341 gcatcgtgta ccgcccgctc tacatgcaga tatttttaga tcaggtcagg ttcgctttct
     2401 tttatgtata cgatttcttt tttggaacgg ctaatttcca atactctttt tttctggatc
     2461 atttttggtc gttatccgtg gaggaacaat tctatatctt ttggccgttg tccgtcctgc
     2521 tggttcctga aaagtcactc aagaaacttt ttttaggttt tattgtattg ggacccattt
     2581 tgcggcttgc cgtctatttc attcatcagt cgggtgtatt ccgttttctt gctgatactg
     2641 ctccgcttgc catcttcccc cttcctttca gctatatcga tgcctttgct tttggggcct
     2701 atatcagccg tttcactatt ccaaaagcca aaactcagtt ctttctgtta ttagggatta
     2761 tgccaattat tggatacggc gcgcaatatc ttgcaacagg caaaattggg acagtttcag
     2821 ctctcggcta cccgcttctt ttgccggagt cctatcaatt catttgggga tatacacttc
     2881 ttaattactt cttcgcggtc acgatctatt ccgtcgtgaa cgatagaatt ttcatcagat
     2941 ttctggaatc gccgccattg caatacctag gaaaaatttc ctacgggtta tacgtctatc
     3001 actttcccat aatctggttt tccatgcgcc tgcgtgactt cggtatcgtg gatccgtttg
     3061 ccaaacctgt gattgcagtt gtctccttcg tcgcgacgtt gctcgtggca tccatcagtt
     3121 ataagctgct ggagcaacct ctgctcaatt tgaaggatcg cttcttcaac cgtagggaaa
     3181 cttcggtgcc agagggtgta aggctaccac ttaaatcaga cctttcatga acagtcgagt
     3241 cttacaaact gctccaggca aagctaacac gttttcattg tcggaaaacg tcttgaccgg
     3301 tttaattctc attgcgggtc tttttctacg tctcaggcaa tacttgacag gtcgctcgct
     3361 ttggctggat gaggcaatgc tggctttgaa cattgttaat cgcg
//
LOCUS       DASTJP010000727         6770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_380995, whole genome shotgun sequence.
ACCESSION   DASTJP010000727 DASTJP010000000
VERSION     DASTJP010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380995"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFQ23_21235"
     CDS             <1..825
                     /locus_tag="VFQ23_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9909187.1"
                     /translation="VMWYNPTVLSDAGVEVPTTWDEFFTACDAIMAADKICLALGPQW
                     TAMHLFENVMIGSLGADGWNALWTEGADWSSAEVTAALENFSRALSYTNSDAAQLPDW
                     QPAAQLVTNGDAAFNIMGDWADGYFENAAPDGLGLTAHEDFDWAPVPGTDGIFVFLSD
                     SFVLPANAANPDATNAWLAVAASKAGQEAFNPVKGSICARTDCDQSLFNEYLQSAASD
                     WSTDTVVGSLTHGVVANDAWKSKIDTALGLFLASPTDVASFQTALADACASDGVCQ"
     gene            985..2061
                     /locus_tag="VFQ23_21240"
     CDS             985..2061
                     /locus_tag="VFQ23_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9909188.1"
                     /translation="MSRGFSLHTPRLILASLLILTGIFSIRFLSANELEIAGNNFTSI
                     GLLISALVFYVGVMMIVAMFENETFLSFALLFPSIVAVAIFVYGFIAWSVRVSLSKWK
                     GLNPDYTFVGLQQYTDLIFDDQRFLIDVRNTAVFTIGFILGCLVLGLGLAILLDQKLK
                     GESLFRSIFLFPMSISFIASGVVWAWLMNPATGSRTTGLNLLFETLGLDFLTSTWHAT
                     PPPWGMAYTVIPAIWQLSGFAMALYLGGIRSVSEEIREAARVDGATELEIYRYVILPM
                     LRPVTLSAVIILGHMSLKIFDLIVALGRKDQRLDVPGIYMWTTTFDGTNYAQGAAIGI
                     LMLISVAILVVPYLINSMKAEAEL"
     gene            2058..2879
                     /locus_tag="VFQ23_21245"
     CDS             2058..2879
                     /locus_tag="VFQ23_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9909189.1"
                     /translation="MTRQNAVRPLFYLVLLTLAVFYLLPVYLMVLTGLKPFDEVDLKT
                     MWNLPSVLRIENFIAGYQQLAPNLRNSLLMVIPEALLSALLGSFNGYLLSKWKFRGSN
                     VIFTLILFGMFIPYQSILIPLVKFMQYINLYGDLPGLILVHIIYGIPITTLIFRNYYA
                     GIGTEIVEAARIDGASLFGIYRHIILPLSAPSFAVVIIWQFTQAWNEFLFAVILTNQS
                     NWPVTVALNNLAGSQLVQWNVLMAATFLAALPTLLVYIFLGRFFLRGLMAGALKG"
     gene            2911..4431
                     /locus_tag="VFQ23_21250"
     CDS             2911..4431
                     /locus_tag="VFQ23_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9909190.1"
                     /translation="MKPPILEMKNITKEFPGVKALKDVSFQVAEGEIHCLVGENGAGK
                     STLMKVLSGVYPIGSYTGDILFNGEIQNFRSISDSEKAGIAIIYQELALVPEMTVYEN
                     IFLGHEIANGFTVNWNETIKRANEMLKKVRLEVNPEVKVKNLGVGKQQLVEIAKALSK
                     DVRLLILDEPTAALNENDSENLLNLLRDLKNHGVTCVLISHKLKEVIEIADTVTVLRD
                     GKTVVTLDAKKGEVSEGVLIKHMVGREIDNIFPSREHKGSGEVVLEIKNWSAYNPELG
                     RQILKNVNVSVRKGEIVGFAGLMGSGRTELALSLFGNPDGYRINGELYIKGQRREFNH
                     PQEAIAAGMAYVTEDRKGDGLILIQDVKQNITLANLREISSSGVVNKNAEVKVANEYK
                     TSLNIKTPTVEQKVVNLSGGNQQKVSVAKWLFVRPDILILDEPTRGIDVGAKFEIYTI
                     MTRLVQEGMSIIMISSELPEVLGMSDRIYIVAAGKIAGELPIEEATQEKIMHMATN"
     gene            4442..5626
                     /locus_tag="VFQ23_21255"
     CDS             4442..5626
                     /locus_tag="VFQ23_21255"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909191.1"
                     /translation="MSFFNDLRRVLGQNIRDYGMFIALFIITTIFSIATDGLFISSRN
                     ISNLLNQAGYIAVLAVGMTLIIIIRHIDLSVGFLAGFLGAVAAIAMVSWGLPVYVVIP
                     LVLVLGAIAGLITAFPVAQLGIPSFVASLAGWLIYRGALLLVTLRTGTIVIPDDAFNA
                     IGNGYIPDIPGLEFLPGVHKLTLILGLIAIILFIQNDLSARRKKQIYNFEVLPINMFI
                     LKLVSTSVLMGLVTWILAGYNGLSWTVVIVLIVVGIYHFIMSQTVLGRHIYAIGGNPE
                     AAQLSGINVKKLTFIVFASMGMLSALSGILFASRLQSATTTAGTLFELDAIAAAYIGG
                     VSAAGGVGNVPGSLIGALVYISLTSGMNLMGIDISSQYIVRGAVLALAVIFDVATRRS
                     AR"
     gene            complement(5653..>6770)
                     /locus_tag="VFQ23_21260"
     CDS             complement(5653..>6770)
                     /locus_tag="VFQ23_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909192.1"
                     /translation="GGLMLQPNAGDVYELLSLKTTNVILELGDSTIHTLQDVPACDAV
                     VCFAPNHFLHNIITSILILPTSPQMDGLRQTVREVYLYWRPDWDGSFNISALSFFVLN
                     LFFIVLGISVAWGKLKMPGLTPLAIFIFYDLSTGLARTSGGRYLVPMDWIISIYFLLG
                     VFQSIVWFANQIGVTWNLFSSPVSQNNSQNTSNPKLSSVILIVAALFGLGSLIPLSES
                     FQAARYQNTDVAQSLIEHEPTITSAGMSISDIDAFLMNPNAELLIGRILYPRFYPIDR
                     GEIFIQPYVTMGFPRTAFIFIGPHGEQGVVLPGDTPKYFPHAADALVLGCREEKYLDA
                     VAVIFLDDSQAMYTRSPKSELTCPLQQPVCNSNRVCQ"
BASE COUNT         1686 a         1506 c         1721 g         1857 t
ORIGIN      
        1 gtgatgtggt acaaccccac agttcttagt gatgccggtg tcgaagttcc tacaacgtgg
       61 gacgagttct ttacagcctg cgatgcgatc atggcagccg ataagatctg tcttgcgctt
      121 ggcccacaat ggactgcaat gcatctgttt gagaacgtaa tgatcggctc tttgggtgca
      181 gatggctgga atgctctgtg gacggaaggt gccgactgga gcagcgctga agttacggcc
      241 gccctggaaa acttctcgcg cgctctctcc tataccaata gcgatgccgc acaattgcca
      301 gactggcagc ctgccgctca actggtaacc aatggtgacg ccgccttcaa catcatgggc
      361 gattgggcag atggctactt tgaaaatgcc gccccggatg gccttgggct aacagctcat
      421 gaggatttcg attgggctcc cgttcctggc acagatggca tctttgtgtt cctgtcagat
      481 agcttcgtgc tgcctgcgaa tgcagcgaac ccagatgcga caaacgcctg gttggcggtt
      541 gccgcctcca aggcaggtca ggaagcattc aacccggtca agggctctat ttgtgctcgc
      601 acagactgcg atcaatccct gttcaacgaa tatctgcaat cggctgcctc cgattggagc
      661 accgatacag tggttggaag cctgacgcac ggcgtggtcg cgaacgatgc ctggaagagc
      721 aagattgata ctgctctcgg gctgttcctc gcaagtccga ccgacgttgc cagtttccaa
      781 actgctttag cagatgcctg tgcatctgat ggtgtatgcc aataggcaaa tggttgtgta
      841 agtagaatga tgggcggctc agtatcgagc cgcccatttg tcattaattg tttttatgct
      901 aagactccca aggtgcacat tccggagccg ttcgattaaa atgacgtatc atagggcata
      961 ctccaaacga caaagaggta gtcaatgagt cgcggtttct ctctgcatac gccacgctta
     1021 atactggcca gcctactgat acttacgggg atattttcaa tccgttttct ctctgcgaat
     1081 gagctggaaa tcgccggaaa caattttaca tccattggct tgttgataag cgcacttgtc
     1141 ttttatgtcg gtgtaatgat gatcgtcgct atgttcgaga atgaaacgtt tctctcattt
     1201 gctttattat ttccatccat tgtggcagtt gccatttttg tatatggttt tattgcctgg
     1261 tccgttcgag tgtctctgag caaatggaag ggacttaacc ctgattacac atttgtaggt
     1321 ctacagcagt acacggatct aatttttgac gaccagcgct tcctgatcga tgtgcgcaat
     1381 acagcagttt ttacgattgg tttcattctg ggttgcctgg tgttgggact tgggctggcg
     1441 attttgctgg atcaaaaact taagggagaa agtttatttc gcagcatttt tctttttccc
     1501 atgtcaatct cttttattgc atccggtgtg gtttgggcat ggctcatgaa ccccgcgact
     1561 ggcagccgaa cgacaggtct gaatcttctg tttgaaactt tgggcttgga ttttctcacg
     1621 agcacatggc atgctacgcc gcctccgtgg ggtatggcct atacggtcat ccctgccatc
     1681 tggcagctct caggctttgc gatggcgctc tacctgggag gcatccgaag tgtttcggag
     1741 gaaatacgcg aagcagcgcg ggtggacgga gctaccgagc tcgaaattta ccgttatgta
     1801 attctcccta tgcttcgacc tgttacattg agcgcagtca ttattcttgg acacatgtcg
     1861 ttgaaaattt tcgatctgat tgttgcgctg ggacgtaaag atcaacgtct tgatgtacct
     1921 ggaatttata tgtggaccac aaccttcgat ggcacaaact acgctcaggg cgcagcgatt
     1981 ggtatactca tgttgatcag tgtggcgatt cttgtcgttc cttatttgat taacagtatg
     2041 aaagcggagg cagagctatg acgcgtcaga atgccgtcag acccctgttt tatttggtat
     2101 tgctgaccct ggctgttttt tatttgctgc cggtttacct gatggtactc acaggtctca
     2161 agccttttga tgaagtcgat ttgaagacca tgtggaacct gccaagcgtc ctacgtattg
     2221 agaacttcat tgcaggttat caacaacttg cacccaacct gcgaaatagc ctgcttatgg
     2281 tgattccaga agctctgtta tccgctctgc tgggttcctt caatggctac ctcctttcca
     2341 agtggaaatt ccgcggttcg aacgtaatct ttacattgat cctatttgga atgttcattc
     2401 catatcagag catcctgatt ccgcttgtga agttcatgca atatatcaat ctgtatgggg
     2461 atcttccagg cttgattttg gtgcacatta tttatgggat tcctatcact acgctgatct
     2521 ttcgcaatta ctatgcaggc attgggactg aaatcgttga agcggcacgc attgatggtg
     2581 caagtttgtt tggtatctac cgccatatta tcctgccgct atctgcgccc agttttgctg
     2641 tggtgatcat ctggcagttt acgcaagcct ggaacgaatt cctgtttgca gtgattctga
     2701 ccaatcaatc caactggccg gtgacggttg cgctcaataa ccttgccgga agtcaactgg
     2761 tgcagtggaa tgtcctcatg gcagctacct ttctggctgc attgccaacg cttctggttt
     2821 atatcttcct gggtcgcttc ttcctccggg gtctaatggc cggtgcgctg aaaggctaat
     2881 tgtgagcatt ttatataaga tggagcaacg atgaaaccac ccatcctcga aatgaaaaat
     2941 atcaccaagg agtttcctgg cgtaaaggca ttgaaggatg tcagttttca agtcgcagaa
     3001 ggggagattc actgtcttgt gggggagaac ggcgccggga aatccacatt aatgaaggta
     3061 ttgagtggag tatatccaat cggcagttat acaggtgaca tcctttttaa tggtgaaatc
     3121 cagaatttta gaagtatcag cgacagtgaa aaggccggga tcgcgattat ctatcaggaa
     3181 ttggcgcttg tccctgaaat gacggtctat gaaaatatct ttctgggtca tgaaattgca
     3241 aatggcttta cggtcaattg gaatgaaact attaagagag ccaacgaaat gctcaagaaa
     3301 gtcaggctgg aagttaaccc tgaagtaaag gtcaagaacc tgggcgtggg aaaacaacaa
     3361 ctggtggaga ttgcaaaggc cttgagcaag gatgtcaggc tgcttattct cgatgaaccg
     3421 acagcggcct tgaatgagaa tgacagtgaa aaccttttga acttgctgcg ggatctgaag
     3481 aaccacggtg ttacctgtgt tttaatctcg cacaagctaa aagaagtgat tgagatcgca
     3541 gatacagtga ctgtgttgcg cgatggaaag acggtcgtca cgctcgacgc aaaaaaaggt
     3601 gaagtctccg agggcgtgct tatcaagcac atggttgggc gtgaaattga caatatcttc
     3661 cctagtcggg aacataaagg ctcgggtgaa gttgtgcttg aaatcaaaaa ctggtctgcc
     3721 tacaaccccg agcttggccg gcaaattctg aagaatgtaa acgtcagtgt aagaaaaggg
     3781 gaaatcgttg ggtttgctgg tcttatgggg tccggacgta cggaacttgc tcttagtcta
     3841 tttggaaacc cggatggcta tcgtatcaat ggtgaattgt atatcaaggg gcagcgaaga
     3901 gagttcaatc atccacagga ggcgattgcc gcaggcatgg cttatgtcac agaagacaga
     3961 aaaggtgatg gactgatcct gatacaggat gtgaagcaga acatcactct cgcgaatttg
     4021 cgtgaaatct ccagcagcgg tgtggttaat aaaaatgctg aagtgaaagt tgcaaacgaa
     4081 tacaaaactt ctcttaatat caaaacgccg actgtggaac aaaaggtagt caacctgagt
     4141 ggcggcaacc agcaaaaggt atccgtggcg aaatggctgt ttgtccggcc agatatattg
     4201 attttagatg agccaacacg gggaattgac gtcggcgcaa aatttgagat ttacacaatt
     4261 atgacgcgtc ttgtacaaga gggcatgagc ataatcatga tatcgtctga attgcctgaa
     4321 gtcctgggaa tgagcgacag gatctacatt gtcgccgcag ggaaaatcgc cggtgaactt
     4381 cccattgagg aagcgacaca ggaaaaaatc atgcacatgg caacgaactg aggaggcctg
     4441 gatgtccttt ttcaatgatt tgcgaagggt attgggtcag aatatccgtg actacggtat
     4501 gtttatcgcg ttgtttatta ttacaacgat cttttcaatt gcaacggatg gtctgttcat
     4561 ttcatctaga aatataagca atctcttgaa tcaggctggc tatatcgccg tcctggcagt
     4621 gggcatgacc ctgatcatca tcatccggca tattgatctc tccgtcggat ttctggctgg
     4681 ctttctgggt gcggtggcag cgattgcaat ggtctcctgg ggattgcctg tttatgtcgt
     4741 catcccgctt gtgttggtac tcggcgcgat tgccggacta atcacggctt ttccggtggc
     4801 gcagttagga attccttcat ttgtcgcgtc gctggctggg tggttgatct atcgaggcgc
     4861 gttgctgctc gtcacattgc gaacgggtac gatcgtgata ccggacgatg cttttaacgc
     4921 aattgggaat ggatatattc ctgatattcc tgggttggaa tttttgcctg gagtacataa
     4981 gctcaccctc atcttagggc tgatcgctat tattctcttc atccaaaatg acctcagcgc
     5041 gcgtagaaag aagcagatct ataactttga ggttctgccg atcaacatgt tcattctcaa
     5101 gttggtctcc acatcggttt tgatgggtct cgttacatgg atcctggcag gctacaacgg
     5161 tttgtcatgg acggttgtta tcgtgctgat tgtggtgggt atctaccatt tcatcatgtc
     5221 tcagacggta cttggacgac acatctatgc gattggcggt aaccccgaag ccgctcagtt
     5281 aagtggtatc aatgtaaaga aattaacctt tatcgttttt gcttcgatgg gcatgttatc
     5341 ggctctctct ggcatcctct ttgcatcccg cttgcaatct gccacgacta cggcaggcac
     5401 gcttttcgag ctggatgcga tcgctgccgc gtacatcggg ggtgtatccg cagcaggtgg
     5461 tgtgggcaat gtgcctggct cgctgattgg ggctttggtg tatatctctc ttaccagcgg
     5521 aatgaacctc atgggtattg atatttcctc ccaatacatt gtgcgcggtg ctgtacttgc
     5581 ccttgcggtt atctttgacg tggcgacacg tcgcagcgca cgataatctt tcctctttgc
     5641 tttagtaaac cgctattggc agacccgatt gctattgcac accggttgtt gcaacggaca
     5701 cgttaattca gatttgggag agcgggtgta catcgcttga ctgtcgtcca gaaagatgac
     5761 agccacggca tcaagatatt tctcctccct acatcctaaa acgagtgcat ctgccgcatg
     5821 cgggaaatac ttcggggtgt cgcctggcaa aacgacgcct tgttcgccat gcggaccgat
     5881 gaagatgaaa gcggtacgag gaaatcccat cgtaacataa ggctgaatga atatttctcc
     5941 tcgatcaatc ggataaaaac gtggatacag gattcgacca ataagcagct cggcatttgg
     6001 attcatcaga aatgcatcga tatcgctgat tgacatcccc gcgctggtga ttgttggttc
     6061 atgctcgatc agcgattgag ccacatctgt attttgataa cgcgctgctt gaaagctctc
     6121 agagagaggg atcagcgaac ctaacccaaa caaagctgcg acaataagaa tgacacttga
     6181 taattttgga ttcgatgtat tttgagaatt attttggctg acaggcgaag agaaaagatt
     6241 ccaggtgaca ccgatttggt ttgcaaacca tacgatggac tgaaatacac ccaataagaa
     6301 atatattgaa ataatccaat ccatcgggac cagatatcta ccgcctgaag tacgcgcgag
     6361 tccggtggag agatcataga agataaaaat agccagggga gttaatccgg gcatttttaa
     6421 cttgccccat gcgacactga tgcccaacac aataaaaaat aaatttagaa caaagaaaga
     6481 cagagctgaa atgttaaacg agccatccca gtctggcctc caataaagat agacctctct
     6541 tacggtttgt cttaacccat ccatttgagg tgaggtaggc aagataagga tggatgtaat
     6601 gatattgtgt aagaagtgat ttggagcaaa gcaaacgacc gcatcgcaag caggaacatc
     6661 ctgcaaagta tgtattgtgc tatcacccaa ttcaagtatg acgtttgtag tctttagcga
     6721 gagaagttca taaacatctc ctgcattcgg ctgaagcatc aagccaccag
//
LOCUS       DASTJP010000728         7894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1015709, whole genome shotgun sequence.
ACCESSION   DASTJP010000728 DASTJP010000000
VERSION     DASTJP010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7894
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015709"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFQ23_21265"
     CDS             <1..694
                     /locus_tag="VFQ23_21265"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909193.1"
                     /translation="LWFQTPIPILPYNGILKFQSEKNLKQRIDWFIHHFKSRRVDFFW
                     LVHPTSPPDLPNQLMERGLQEIEVLPGMTRNLRNLPELPSLPDDIQARKAMGENDVSA
                     LYDFSAWRWNVPAEYKGTLANILATLELGKPGSNAHLWQTWRDGQAIAKTGMYVSANS
                     AGIYAVATKPEARGLGLARFLTLSALKEAQSLGKTLAVLHSTPMAQSLYRSLGFEAIA
                     DFRVFASVAGHI"
     gene            complement(744..959)
                     /locus_tag="VFQ23_21270"
     CDS             complement(744..959)
                     /locus_tag="VFQ23_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909194.1"
                     /translation="MFDLFRSFLTSQSYGDYLRSRPTARVCEAWRLGESTHETEKTAR
                     LDSAIFAGPTSRPVYVEDPLSAVMGHC"
     gene            938..1735
                     /locus_tag="VFQ23_21275"
     CDS             938..1735
                     /locus_tag="VFQ23_21275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9909195.1"
                     /translation="MSEISQTWHYGLVARHWAEKNTSGPEIAYYLQRIQQYGQPALDA
                     GCGTGRLLIPFLRAGLDVDGCDVSADMLAYCRQSAEREGFTPRLYHQALQQLDLPRSY
                     QTIVACGVFGVGVSRPQDFSALQRFYQHLLPGGVLLLDVKLPYSDDNLWPLWQKKAQN
                     LLPEPWAKNLGKPPEDGREYELHYRLVNVNSLEQQTTGEMRTLLFSDGKLVADDTYRL
                     ISNYYFPNEVRLLLEKAGFTIEAEKGNWTDTDVTADHHTIVFVARKL"
     gene            2547..3026
                     /locus_tag="VFQ23_21280"
     CDS             2547..3026
                     /locus_tag="VFQ23_21280"
                     /inference="COORDINATES: protein motif:HMM:NF025473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abscisic acid-deficient protein Aba4 family
                     protein"
                     /protein_id="HET9909196.1"
                     /translation="MLSTSFFILSNMAVTPFYLLMLVAPRSGLTRRLLGSLWPVALPA
                     LVHVVFVVLIVILIRPDVPGLWRTMYIENGLFSVTTIEFLSRLYGGFPEYATLHGWVH
                     LVVGDMVMARWAYWDALEHGVASWLIALVTVLIGFIGPVGVVIYLVLRMCSHPAADL"
     gene            3340..4218
                     /locus_tag="VFQ23_21285"
     CDS             3340..4218
                     /locus_tag="VFQ23_21285"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET9909197.1"
                     /translation="MSFNNKSRLDPTQVEDRRGRGSGRAIALGGGGLTLVIVLVAMLF
                     GVDLTDLATVDPGQQAPATEYAGGGPDLQAECQTGADANAREDCRIVGFVNSIQAFWA
                     DEFARGGGQYSEADTVLFTGATEAACGYASSAVGPFYCPRDQKVYLDLSFFNELQTRF
                     GAQGGSFAEAYVLAHEYGHHVQNLLGVLSNTSNATGPQSEAVETELQADCLAGVWAYH
                     AAETGYLTQVTNQDIIQALDAAASVGDDRIQSQTQGYVSPETWTHGSSEQRQLALQDG
                     LQSGDIATCDSPGWTP"
     gene            4553..5095
                     /locus_tag="VFQ23_21290"
     CDS             4553..5095
                     /locus_tag="VFQ23_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909198.1"
                     /translation="MNNQRTLTYLKIVIFLALTLAACSGAPQSQDAQHYVGAVDGSDA
                     FIGLVSDGINIRAYVCDGTTDSVNISAWFQGDVSDGSFALASEGGLSLTGQITESGVD
                     GTVGFVDSTQHSFSATSATEPAGLYRLEETVDGNTTVSGWIILPNGEYRGGLSLTNLV
                     GGPPAIIGGPPAIQFRLLFP"
     gene            5285..5968
                     /locus_tag="VFQ23_21295"
     CDS             5285..5968
                     /locus_tag="VFQ23_21295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909199.1"
                     /translation="MNSDGRLEHPFIREWVKANLIGWLLGILFGMFLFHLIITPLVWS
                     FFDVDYDVYSKLEPIFLSFPLGAGVGIFQQRNLERWKIQLSSWALLTTLSLGIPITLI
                     TWVFQTDVMGYRVPIIASIAEIVIVSLSVGGLQAYLLRQSLSRPIRWVQAYLFGSLAI
                     TVIEFILMAVAFLAAEPIQKLLYSVDLWVIVEYREALLLFSIALIFPFLAAFFIGLPT
                     GRMLYNTMS"
     gene            6092..6940
                     /locus_tag="VFQ23_21300"
     CDS             6092..6940
                     /locus_tag="VFQ23_21300"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9909200.1"
                     /translation="MSIESLIQDIVDEIKNVSGVKAIVLGGSRARGTNHSASDIDLGI
                     YYDLRHPLNLAELAKVATKLDDEHRTDLITAIGGWGPWINGGGWLHIQTFPVDFLYRD
                     LDKLNNVITSCLDGKVEIFYQPGHPNGFVSSIYLGEIAICQPLWDPEGIIADLKRKVI
                     PYPSSLQRAIIQNFAWEIDFSIGIAQKSIAKADVVYAAGCCFRGSMCMLQVLFAMNKE
                     YWLNEKGAVAIADNFKIKPVNFQSRVGKIFTLLSDTPDTISQSVSLLKELNAELSDLL
                     QNSTTV"
     gene            6971..7354
                     /locus_tag="VFQ23_21305"
     CDS             6971..7354
                     /locus_tag="VFQ23_21305"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9909201.1"
                     /translation="MKTTMLPEEADAKSPAGANIRFLMDGQTGNMIHSTVPPHQINKA
                     TVHATVSEFWYVLEGHGEIWRNDGKESNVTDLVPGISIDIPVGTAFQYRNISDVELKF
                     ICISMPPWPGESEATFVNGIWQPTV"
     gene            7531..7713
                     /locus_tag="VFQ23_21310"
     CDS             7531..7713
                     /locus_tag="VFQ23_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909202.1"
                     /translation="MNTILLSILLGLSGMVGWGVYDFLGGAVSILPLAFVVNAATSIP
                     GVIVLLVPLECQRGNF"
BASE COUNT         1999 a         1788 c         1964 g         2143 t
ORIGIN      
        1 actttggttc cagaccccca tcccgatcct tccttacaac ggcattctaa agtttcagtc
       61 agagaagaac ttgaagcaga gaattgattg gtttatccat cattttaaga gtcggcgagt
      121 tgattttttc tggctcgtcc atcctacttc acctcctgat ctccccaatc agttaatgga
      181 gcggggattg caggagattg aagtcttacc aggcatgacc agaaatttga gaaacttacc
      241 agaacttcca tccttgcctg atgatatcca agcacgaaaa gccatgggtg aaaatgatgt
      301 gagtgcctta tatgattttt ccgcttggcg gtggaatgta cctgctgagt acaagggaac
      361 tttagccaac atccttgcta ctttggagct tggtaagccc ggctcgaacg cacatttgtg
      421 gcagacttgg cgtgatggac aggcgattgc caaaacaggc atgtatgtgt cagccaattc
      481 agctgggatc tatgcagtgg caacaaaacc cgaggctcgc gggctgggct tggctcggtt
      541 tctaactctg tccgcactaa aagaagctca atcgcttgga aagacattag ccgtcttgca
      601 ctccacacct atggcacaaa gtctttatcg atctttgggt tttgaggcga ttgctgattt
      661 tcgagttttc gcttctgtgg cggggcatat ctaatctaat gctgagcagc tcttccataa
      721 aatccttgcc acaccaacac cgcctaacaa tgccccatca ctgcgctcag aggatcctcg
      781 acgtacaccg gacggctggt gggacccgca aaaatggcgg agtccaggcg agcagttttt
      841 tccgtttcgt gggttgactc tcccagccgc cacgcttcgc agacgcgagc cgttgggcgg
      901 ctgcgtaaat aatcaccata cgattgtgag gtaagaaatg agcgaaataa gtcaaacatg
      961 gcattacggt ttggtggccc gacattgggc tgagaaaaac accagcggac ctgaaatcgc
     1021 ctattacctt cagaggattc aacagtatgg gcaaccggct ctcgatgccg gttgtggtac
     1081 aggtcggcta ctcatcccct tccttcgcgc cggactggat gtggacggct gtgatgtctc
     1141 agccgatatg ctcgcctatt gccgacaatc tgctgagcgg gaagggttta caccgcggtt
     1201 atatcaccaa gcgctacagc agctcgacct gccccgctcc tatcaaacga ttgtggcttg
     1261 tggtgttttt ggcgttggcg tcagtcgtcc gcaggatttc agcgccctgc aacgttttta
     1321 tcagcacttg ctaccaggcg gcgtattatt acttgacgtg aaattgcctt actcagatga
     1381 caatctttgg ccgttgtggc aaaagaaagc tcaaaacctc ttgcccgaac cctgggcaaa
     1441 gaaccttggt aagcctcctg aagatggtcg agaatatgaa ctccattacc gactggtcaa
     1501 tgttaactca ctggagcagc aaaccactgg tgagatgcgc actctattgt ttagtgatgg
     1561 gaagctggta gcagatgata catacaggct catctcaaat tactactttc ctaatgaagt
     1621 gcgtctgttg ctagaaaaag ctggctttac aatcgaggca gaaaaaggca actggactga
     1681 taccgatgta acagccgacc atcacactat agtctttgta gcaaggaaac tataaaatat
     1741 tttacggcac tgtgcaaaat gcgcccaaca cagcccccat cgctccgctc aggggatctt
     1801 cgacgtgcac tagacgtcgg ggactgcccg aagtgcgtgc cgcatttcaa gcatttttct
     1861 cgcttcgggt ttttcctgct cccaggcaga gtccaccatc gaataaaata agctaccgtc
     1921 gattaatcat ctacaaaaaa ggagtttcaa atggcgccaa gaacttgtcc aacgtgtaat
     1981 ggaagtacat gggaacatga atcctgctct acctgtaatc aaagtggaaa aataacagat
     2041 tcgtccactg gggaaatgaa agattgttcg gactgtaatg gaaccggaac gaagcattat
     2101 agatgtacgg agtgtgacgg caaaggcttt atcgatgatt agtagaagca ccatgcagtc
     2161 atcgtgcaac ttattgttac aaattagtct gtggaggctg ccatagcgaa ccgttaagag
     2221 aatggaagag caacgaaaaa gaactaaaaa taattgccgt atgattcaat gtgtatctct
     2281 taagtgagat tctgtttctt aaactggagt gatgatgatc agctgctgaa gttgtgggac
     2341 tgctaccgca cggctcgctc attttcaggc attttgcgtg gcttggagtt ggttccgata
     2401 aaaaggagtt atctcgtccc acgcagatgc cagccgttag cccgcagaag caacgcaaga
     2461 ggtaaattgg tattggacga gatgcacagt caacagagtg cgtcggttca gtgaatgctt
     2521 cgctacagga tcctcccttg gcactcatgc tcagtacgtc cttctttatc ctgagtaata
     2581 tggcggttac gcctttttac cttttgatgt tggtggcacc gcgatcaggt ttgacgcgcc
     2641 gattgctggg ctcgttgtgg ccggtagcat tacccgctct tgtgcatgta gtatttgttg
     2701 tgctcattgt cattcttatt cgacccgatg tccctgggtt atggcgcacc atgtatattg
     2761 agaatggtct tttcagtgtg acaaccatcg agtttctttc tcggctatat ggcgggtttc
     2821 ctgaatatgc aacattacat ggttgggtac atcttgtcgt cggggatatg gttatggccc
     2881 gctgggcgta ttgggatgcg ttggagcatg gtgtcgcgtc gtggttaatt gcgttggtga
     2941 ctgtactcat tggttttatt ggaccagtgg gagttgttat ttatctggtg ttgcgaatgt
     3001 gctctcaccc agccgccgac ctgtgatctg taaaaacata acgcgaccca ttggctttac
     3061 gatttacatt gcttttacca ccgatctaat atagcttccg aaaatccagg tcttgaactg
     3121 cttctgttga cccctgcagc tggtggagtg caaccttcgc ctgatcgagc gcgcggttga
     3181 tgaacgctgg gtggattata atcattcaag gcttgtacac taggtcaagt caaatctgtt
     3241 cccaaaaaga acacaatagt taaaaaataa ttcataacga tctggatctt acacgcgtca
     3301 atttcgcacg ccaacggatc tcagacaaga ggatgaaaaa tgtcattcaa caacaaatct
     3361 cgtttagacc ccactcaggt ggaagaccgg cggggccgcg gatcaggccg agccatcgct
     3421 ttgggtggcg gagggcttac tctggtcatt gtgttagtcg caatgctgtt tggcgtggac
     3481 cttactgacc tggcgactgt tgatcccggg caacaagcac cagcaaccga atacgcgggt
     3541 gggggccccg acttgcaggc ggaatgccag acgggagcag acgccaatgc ccgggaggac
     3601 tgtcgtattg tgggcttcgt caatagcatc caagcgtttt gggcagacga atttgcccgc
     3661 ggtgggggtc agtattcgga ggccgataca gtgcttttca ccggggccac agaagcggct
     3721 tgtggatacg ccagttccgc tgtgggaccg ttctactgtc ccagagacca gaaggtctat
     3781 ctggacctca gcttcttcaa tgaactccag acacgctttg gcgctcaggg tggttcgttc
     3841 gctgaagcgt atgtgcttgc ccatgagtac ggccatcacg tgcagaacct gcttggtgta
     3901 ttatccaata cttcgaatgc cacaggacca cagagcgaag ccgtcgagac ggaactccag
     3961 gccgactgcc tggccggtgt ctgggcatac cacgctgctg agactggtta cctgactcag
     4021 gtgacgaatc aggatattat ccaggctttg gacgccgcag cttccgtagg tgacgaccgc
     4081 atccagagcc agacgcaggg ctacgtctca cccgagacgt ggactcatgg ctcttctgag
     4141 caacggcagt tggcgctcca ggacggtctg caaagcggcg acatcgccac ctgtgattcg
     4201 ccagggtgga cgccatagac atacaataaa taatacatca ctgattcaat ccatcattgc
     4261 tgggaatgct ctgcctgacc aagaagacgt aacaaaaagt gcccgatggc ttgccttata
     4321 tgcctgccat tgggcacttt gcatggattg tcagtttttc tccgaatact tgcaaatcat
     4381 aatttgatat tgcaagcatt aaatacattt ccagttccat agctagaaaa aggttcagtc
     4441 taacctccct tacttaacta tttaaaaaga gtgctggtag aatcaaattc caaccaaaga
     4501 cagctattgc tgttagtaac agttgcctgt tacaactttt ctcagaggca aaatgaacaa
     4561 ccaacgcaca ttgacctatc tgaagattgt aatatttctt gcactaactc ttgcagcatg
     4621 ctcgggtgcg ccccaatcac aagatgccca gcattacgtg ggcgcggtgg atggatcgga
     4681 tgccttcatc ggtttggtga gcgacggcat caatattcgc gcgtatgtgt gtgacggcac
     4741 gacggattcg gtcaacatct ctgcctggtt ccagggggat gtttcagatg gatcctttgc
     4801 tttggccagc gagggcgggt tgagtcttac gggtcaaatc acggaaagtg gggttgatgg
     4861 tacggtcggg tttgttgaca gtacccagca ttcctttagc gcaacgtcgg ctactgaacc
     4921 tgcagggtta tatcgcctgg aagaaaccgt tgatggtaac acaactgtta gcggatggat
     4981 tatcctgcca aatggagagt atcgcggcgg cctaagcctt accaatttag ttggtggtcc
     5041 ccctgccatt attggaggac cgccggcgat ccaatttcga cttttgtttc cttgatattc
     5101 tggtctgagg cataaaacca caaaattcat ctaccgtgtg cggcatatag gcatgcaata
     5161 atctgcgtgc gtccatgtca aaagtgattt tgcctaaatc catttcggta cttgtcaacc
     5221 ttggtgaagg aatcgtgcgt cgttagtcat ccatctcaat tgttcacacg tcgatggaaa
     5281 aacaatgaac agtgacggtc gattagaaca tccgtttatc agggaatggg taaaagccaa
     5341 tctgattggc tggctattgg gcatcttgtt tggtatgttc ttgttccatc tgatcattac
     5401 tcctttggtg tggtcatttt ttgatgtcga ttacgatgtc tatagcaaat tggagccaat
     5461 ctttctttcc tttcctttag gtgctggtgt gggaatcttc cagcagagga atttagaacg
     5521 ttggaagata caactatctt cctgggcatt gctcacaacg ctgagtcttg gtattccgat
     5581 aacccttata acctgggtat ttcagactga tgtgatgggg tatcgtgtcc ctattatcgc
     5641 cagcattgcg gaaatagtta ttgtcagcct cagtgttgga ggattacagg cttacttgct
     5701 tcgacaatcg ctttcccgac ccatccgatg ggtacaagct tatctatttg gttctctcgc
     5761 tattacggtt atcgaattta tcctgatggc tgttgcgttt ttggcagccg agcccattca
     5821 gaagcttctt tattctgtcg atctttgggt gatcgtagaa tatcgggagg cactgttgct
     5881 gttttctata gcgcttattt ttcctttcct ggctgcgttc ttcattggtc ttccaacagg
     5941 aagaatgtta tacaatacta tgagttgaac atccatgcgc gatggcgaag aaaatggatc
     6001 agcatggtaa aaaagcttgc ccccgaatat atttgggccc atgttgcgtc caacggccat
     6061 taggcagata gaaatatatt cgtaggtgtt catgagtatt gaatcgctca ttcaagacat
     6121 tgttgatgaa atcaaaaatg tatcaggagt taaggcaatt gtgctcggtg gttcccgcgc
     6181 cagaggaacg aaccattctg catctgatat tgatttgggg atttattatg acttgcgcca
     6241 tccattgaat ttagccgaat tagcaaaagt agcgacaaaa ctcgacgacg aacatcgaac
     6301 agaccttatc acagccatcg gtggttgggg cccttggatc aatggcggtg gttggcttca
     6361 tattcagaca tttccggtag actttctata cagggatttg gacaaattaa acaatgtgat
     6421 tacttcttgc ctggatggaa aagtagaaat tttttaccag ccaggacacc caaatggctt
     6481 tgtttcatcg atttatcttg gagaaattgc aatctgtcag ccactttggg atcccgaagg
     6541 aatcattgcc gatttgaaac gcaaagtcat tccatatccc tcctctttac aaagagcgat
     6601 catccaaaat tttgcatggg aaatcgattt ctcgatagga attgcccaaa aaagtattgc
     6661 caaggctgat gttgtgtatg cggcaggatg ttgtttcagg ggttcgatgt gtatgcttca
     6721 ggtactattt gcgatgaaca aagaatattg gttgaatgag aaaggggctg ttgccattgc
     6781 agataatttc aaaataaagc cggtcaattt tcagagtcgg gtcggaaaaa ttttcacatt
     6841 gttaagcgac acgcctgata ccataagcca atccgtgtct ttgttgaaag aattaaatgc
     6901 agaattgagt gatttgttgc agaatagtac gacagtataa aatctgcaga tgtaaattga
     6961 ggagaaatat atgaagacaa caatgcttcc agaggaagcc gatgcgaaat cgcccgcagg
     7021 tgcgaacatt cgcttcctta tggatggcca aacgggcaac atgatccata gcacggtccc
     7081 tccgcaccaa ataaataaag cgactgttca cgcaacagta agtgaattct ggtatgtatt
     7141 ggagggacat ggtgagattt ggagaaatga cggcaaagag agcaatgtaa ccgatcttgt
     7201 tcccggcata tcaattgata ttccagtagg aacagcattt caataccgaa atatttcaga
     7261 cgtagaatta aagttcattt gcatcagcat gccaccttgg ccaggcgaat cagaggccac
     7321 ctttgtcaac ggtatatggc agccgacagt ttgaggtttt tctatcactc atacgagcag
     7381 aatagagaga agaaggctaa aaaggcgtcc cgggagaaca ccgggatagt gctcaattct
     7441 gcccagccgt actctgaagc ggcatgacgc ttgccgctgg gctctgacta tagccgcttc
     7501 aacaaaacac caatgaatta ttcaaaaccg atgaatacaa tcctcctaag catattactg
     7561 ggactcagcg gaatggtcgg ttggggggtt tatgattttt tgggtggcgc ggtgtctatc
     7621 cttccgcttg catttgtggt taacgccgcc actagcattc ccggtgttat agttctcctc
     7681 gttccactgg aatgtcagcg aggtaatttc tgaagaaatc gattggctat tgagcgcgtt
     7741 acttgtgaaa tctggaattg tttagtcttg ttactatctt cctttgtagc aaagcaagac
     7801 attagaataa tgaacagttc gacaagaaca aagcatgtga ttctcttgat gggtattatt
     7861 gaggttggag ccgtggcact tgtaaactat ggtt
//
LOCUS       DASTJP010000729         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_909680, whole genome shotgun sequence.
ACCESSION   DASTJP010000729 DASTJP010000000
VERSION     DASTJP010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909680"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFQ23_21315"
     CDS             complement(<1..591)
                     /locus_tag="VFQ23_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909203.1"
                     /translation="MIRKLLERILPWMARIGYDENDSDESRLQKTMMALGSLMFTTAG
                     GLWGILYFLLGEKAASAIPLGYTLISFLSVLIFHWTRWYKFLLFTQLLLILFLPFLLM
                     IALGGFIKSSAVILWSLLSPLGALLFGDPRQAMRWLAAYLGLTIISGYFEFYPLSVSS
                     LSPTLIVIFFVMNIGTVSAISIFLMAYFVNQKNMLFE"
     gene            1075..1482
                     /locus_tag="VFQ23_21320"
     CDS             1075..1482
                     /locus_tag="VFQ23_21320"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET9909204.1"
                     /translation="MRRNLLLMSLILTLILTSCGGSDRPTTTIDVTMTDFMFQPNQFI
                     VPAGQEITVNTRNNGVVVHNFVIMKLGTTAGPTFEDEDEPNVYWKVEMTSGNSTTTSF
                     IAPTEPGEYEVICSTQGHIASGMVGKLVVVAGE"
     gene            1515..2315
                     /locus_tag="VFQ23_21325"
     CDS             1515..2315
                     /locus_tag="VFQ23_21325"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9909205.1"
                     /translation="MQKPLITSLSNPLIKQARSLHQKKARSESGLFLVEGIHPVGEAI
                     EAGWDVKSILFAPELLTSNFARDLLSRQSVESQPVTAQVMESLAGKENPQGILAVVRQ
                     KETHFADVAFVEKSVALVSPQDPGNVGTILRTLDAVGAEALFLLDSQDNAGGSVDLYH
                     PTVIRASMGTIFWKPVIQSSFERFIQWVRQGTHQLIGTSAHGDVDYRTLIPQSPWILV
                     LGNEQKGLSADQMKACDVTVSMPMKGRVSSLNLAVAAGVLLYQFTEKT"
BASE COUNT          693 a          568 c          607 g          628 t
ORIGIN      
        1 ttcaaataac atatttttct ggttcacaaa gtaggccata agaaatattg aaattgcgga
       61 gacggtgccg atgttcataa caaaaaagat gacaatgagg gtgggtgaga gtgatgaaac
      121 cgataaggga taaaattcaa aatagccact aatgatggtc agtccgaggt aggctgccag
      181 ccagcgcatt gcctggcggg gatctccaaa taagagtgcg ccaagcggag acaaaagtga
      241 ccataaaatg accgcgctgg atttaataaa gcctcccagt gcaatcatga gcagaaaggg
      301 cagaaatagg atcaagagga gttgagtgaa gagaaggaat ttgtaccacc gcgtccaatg
      361 aaaaattaga acgcttaaaa aggaaattaa ggtatagcca aggggaatgg cactggctgc
      421 cttttcaccc aacaaaaaat acaggatgcc ccacagtccg ccagcggtcg tgaacatgag
      481 ggaccccagc gccatcattg tcttttgtaa acggctttca tcgctatcat tctcgtcgta
      541 gccgatgcgc gccatccagg gcaagatacg ttccagcagt tttcgaatca aattaaagcc
      601 tcctttggca cttctcctga atgtacaatc cggttagtga tatatgctac gtttgaatcg
      661 ttttatagta tgtacgcacc ctgatcccgc atgaactaca ggttaagtgc aaatacattt
      721 tcgaaaagcc aggtgcctta gatcttattt cttaattatt gaaacggtca taggagtggc
      781 tttgccgaaa ctggagcggt aatacgggaa taggtcgcaa ttcaggagct gcggcatcga
      841 ccaaactccg tccacgaagt tgcgatagct ctgcatcctc atcgcgcggc aaaggacgat
      901 gttagctgaa gtcattttga tccaataagg tcacaaactt tgctgaattt agatagtatc
      961 tcaacaagcc gggcgccgat ccatagcggt gacggctgca gtaatattct gagagtaaac
     1021 tttcattttg gattatatta caatatatct acccaaaatt ttgaggagaa gtaaatgaga
     1081 agaaatttgc tattgatgag cctcattctc actttaatac ttacatcctg cggcggttca
     1141 gatcgcccta caacgacaat cgatgtaacg atgaccgatt ttatgtttca gccgaatcaa
     1201 ttcattgtgc cggctggtca ggaaatcacg gtcaatacga ggaataatgg agtggtcgtc
     1261 cataattttg tcattatgaa acttggaaca actgccggac caacctttga agatgaggat
     1321 gaacccaacg tatattggaa ggttgagatg acttcaggaa actctacaac cacttccttt
     1381 atcgcaccga ctgaacccgg tgaatatgaa gtgatttgca gcacccaggg tcatattgct
     1441 tctggaatgg ttggcaaatt ggtcgtggta gctggtgagt aagagcggag caatgctccg
     1501 cctctaaatt gaatatgcaa aagccattga taaccagcct aagcaatccg ctgatcaagc
     1561 aggcgcgttc cctgcatcag aaaaaggcac gcagcgaaag cgggcttttc ctcgtcgaag
     1621 gaattcaccc tgtgggtgaa gcgatcgagg ctgggtggga cgtcaaatcc attctgtttg
     1681 cgcccgagct tttgacaagt aattttgcaa gagatctgct gtcacgtcag tccgttgagt
     1741 ctcagccggt cactgctcag gtgatggaat cgctggctgg aaaagaaaac ccccagggca
     1801 tcctggcggt cgttcgtcaa aaagaaactc actttgccga tgttgccttc gttgaaaaat
     1861 ctgttgcatt ggtctcccca caagaccccg ggaatgtggg gacaattctg cgaacgcttg
     1921 atgcagtcgg agcggaggct ttgtttttgc ttgactcaca ggacaacgcg ggtgggagtg
     1981 tggatctgta tcatcccact gtcattcgtg ccagcatggg gacgatcttt tggaagccag
     2041 tcatacaatc ttccttcgag cgattcattc aatgggtgcg acaaggtacg catcagttaa
     2101 tcgggacgtc cgcacacggc gatgtggact atcgaaccct tattccgcag tctccctgga
     2161 tcctggtgct cggaaatgaa caaaagggtt tatccgccga ccaaatgaaa gcctgtgatg
     2221 taactgtatc tatgccaatg aaggggaggg tcagctcatt aaacctggcc gtcgcggccg
     2281 gtgtgttgtt atatcagttt acagaaaaaa cataggggcg aagtatggct tcgcccctat
     2341 aagtgggtat ggatgctcaa ctaaaatcaa cgattagaat tcgtcttcgt cctcatccca
     2401 aagttcatca tcatcctcgc catcctcctc ctcccattct tctgactctt cgtcatccca
     2461 ttcctcttca gcttctgatt cgccgctggg agaata
//
LOCUS       DASTJP010000730         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_752277, whole genome shotgun sequence.
ACCESSION   DASTJP010000730 DASTJP010000000
VERSION     DASTJP010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752277"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFQ23_21330"
     CDS             complement(<1..634)
                     /locus_tag="VFQ23_21330"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9909206.1"
                     /translation="MRRVFLILAVLGLCSSAFAQKPRLIKEANDYFAHGKYRAALQYY
                     KQGGNAQTWDGDTRLRVAISQFEINDIDGAIPLLVSLIQEGKTDPQVFLYLARCYHHK
                     NQFAYAVNYYKQFLRKFDDPTPLREWVKDEVTRCANGISLRYGVERAYVENMGAAINS
                     VGEDFAPTPSPNYQERLYFTSGREDSKGGMLSNDGSTDQKFGSYRSDIYYT"
     gene            complement(655..2052)
                     /locus_tag="VFQ23_21335"
     CDS             complement(655..2052)
                     /locus_tag="VFQ23_21335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9909207.1"
                     /translation="MTTVDLIMPKMGESIMEATILKWVKKEGDPIAVDETILEIATDK
                     VDSEIPSPVAGTLGKILFKENDVVPVGTVIAQIQVAGEAVKPKPTPAVSETQTQTQTQ
                     TQNVIQAQKQNGLSNGQKQTPPPAPKKVVSPTAPVATAVAARAGHRFYSPLVRNIAQQ
                     EGIGAAELETIEGTGLDGRVTKNDLLSYLSNRSTPQQPVAPVSPAVTVEPAAPVSPVS
                     PVSPVSPALAGDNVEIIEMDRMRQLISDHMVRSKHTAPHVTSFVEADVTALVQWREKV
                     KDDFFKQYGQKITFTPIFIEAVVRAIRDYPMINISVDGTRIIRKKDINIGMAAALPSG
                     NLIVPVIRNADYMNLMGLTKSVNDLADRARKGQLKPDEIQGGTFTLTNVGTFGNVMGT
                     PIINQPQVAIMATGAIRKKPAVIETKQGDVIAIRHMMFLSLSYDHRVVDGALGGSFLR
                     RVADYLEGFDPERPV"
     gene            complement(2143..>2385)
                     /locus_tag="VFQ23_21340"
     CDS             complement(2143..>2385)
                     /locus_tag="VFQ23_21340"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET9909208.1"
                     /translation="AMAIGARETFKSDYAIAVTGIAGPSGGSPEKPVGTVWIAVADEN
                     GAVTKRHSFGKDRLRNIELSAVYALDLLRKQLSAAS"
BASE COUNT          564 a          625 c          574 g          622 t
ORIGIN      
        1 ccgtgtaata aatatctgat cgataggatc caaacttctg atctgtcgag ccatcgttgg
       61 acaacattcc tcctttgcta tcctcacgac cagaagtaaa atataaacgc tcctggtaat
      121 tcgggctggg tgtcggtgca aagtcttccc ccactgagtt gattgctgca cccatgtttt
      181 ctacataggc cctttctacc ccataccgca gactgattcc attggcacaa cgcgtgactt
      241 catcttttac ccattcacgc aatggtgtgg gatcgtcaaa ttttcggaga aactgtttgt
      301 aataattgac agcataagca aactgattct tatgatgata gcatcgtgcg agatataaaa
      361 atacctgtgg atcagtcttg ccttcctgga tcagactgac cagcaacggt attgctccat
      421 caatatcatt gatctcaaat tgactgatgg ctacgcgcaa cctggtgtcg ccatcccagg
      481 tctgggcatt gccaccctgc ttgtagtact gcaatgcagc acgatacttt ccatgtgcga
      541 aatagtcatt ggcttccttg atcagtcttg gcttctgcgc aaaggcgctc gaacacaaac
      601 caagtacagc aagaatcagg aagacacgac gcatagtgta attaaagttg gagatcaaac
      661 agggcgttcg ggatcaaagc cttccaggta atctgctacc cgtcgcagaa atgaccctcc
      721 aagtgctcca tcgacaaccc gatgatcgta cgataacgaa agaaacatca tgtgccgtat
      781 tgcgataaca tctccctgct ttgtttcaat gaccgccggc ttcttccgaa tcgccccggt
      841 agccataatg gcaacctgtg gctgattgat gatcggtgta cccatgacat tgccaaaggt
      901 gcccacattc gtcaatgtga aggtgcctcc ctgaatctca tcaggtttga gttgaccttt
      961 tcgcgcgcga tcagccagat cgttcacgga ttttgtcagg cccatgagat tcatatagtc
     1021 ggcattgcgg atgacaggga caatcagatt tccacttggc aatgcagcgg ccatcccaat
     1081 attgatatcc tttttgcgga tgatgcgtgt tccatcgaca gaaatattga tcatcggata
     1141 atccctgatc gctctgacaa cggcttcaat gaagattggt gtaaaggtga ttttctgtcc
     1201 gtattgctta aaaaagtcgt ccttgacttt ttcccgccat tgcacgagtg cagtcacatc
     1261 agcctccaca aacgaagtca catgtggcgc ggtgtgcttt gagcgcacca tatgatcaga
     1321 gatcaactga cgcatccgat ccatctcaat gatctcgacg ttatcacccg ctaaagccgg
     1381 agaaaccggg gaaaccggag aaaccgggga aaccggggcc gcgggttcaa ctgtaacagc
     1441 aggtgaaaca ggtgcaacgg gttgttgcgg agtgcttctg ttgctgagat atgagagcag
     1501 gtcattcttt gtgacgcgac catcgaggcc ggtcccctca attgtttcaa gttcagccgc
     1561 cccaatacct tcttgctgtg cgatattccg caccagcggt gaataaaacc ggtgtccggc
     1621 gcgagccgcg actgcagttg ctaccggagc cgtgggactc accactttct tcggcgccgg
     1681 cggaggcgtt tgcttctgcc cattcgacag gccattctgc ttttgcgctt gtattacgtt
     1741 ttgcgtttgt gtttgtgttt gagtttgagt ttccgaaacg gccggcgtcg gcttcggctt
     1801 cactgcttca cccgccacct gaatctgcgc gatgacagta ccgactggca caacgtcgtt
     1861 ttctttgaac aggattttcc ccagtgtgcc agcgaccggt gaaggaatct cagagtccac
     1921 cttgtcggtg gcaatctcga ggattgtttc atccacggcg atggggtccc cttctttttt
     1981 gacccatttc aggatcgtgg cctccatgat actttcgccc atcttgggca tgattaaatc
     2041 tactgttgtc atgatcgcaa aggtaagcat tcggggaagt cgcgtaaggt gggggaatgg
     2101 agaagagcca acaagtttag ttcccacggt ttagcgagca actcaactgg ccgctgacaa
     2161 ctgttttcgc aggaggtcca gggcgtagac tgcactcaat tcaatgttgc gcagacgatc
     2221 cttaccaaaa gaatgccgct tcgtcacagc tccattttcg tcagcgactg caatccacac
     2281 tgtaccaaca ggcttttcag gagatcctcc ggatgggccg gcaatccccg tgaccgcaat
     2341 cgcatagtca gatttgaatg tttcccgtgc accaattgcc attgc
//
LOCUS       DASTJP010000731         8476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1332462, whole genome shotgun sequence.
ACCESSION   DASTJP010000731 DASTJP010000000
VERSION     DASTJP010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332462"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFQ23_21345"
     CDS             <1..220
                     /locus_tag="VFQ23_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909209.1"
                     /translation="ERYTQDVRVLRNVMQQRSASQLDIIRVRTEEEDSFTVEIHIQAL
                     SLRGGVKRRRSREASSHHRDCFELTLSQ"
     gene            complement(232..894)
                     /locus_tag="VFQ23_21350"
     CDS             complement(232..894)
                     /locus_tag="VFQ23_21350"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9909210.1"
                     /translation="MTRKLIDLSHEVEHGMITYKGLPAPIICDFLSREDSRKHYAEGT
                     EFNIGKIEMVANTGTYVDSPFHRFADGMDLAELPLESLADLESTVVYAQNFGRAINAK
                     AFKDIDVKGKAVLIHTGWARHWRTDQYFEGHPFLTRDAAEFLAESGAAFVGIDTYNID
                     DTTDGTRPAHTVLLGNRIPICEHMCGLENLPGSGFRFHAAPVKVKAFGTFPVRAYAVV
                     GE"
     gene            complement(959..1759)
                     /locus_tag="VFQ23_21355"
     CDS             complement(959..1759)
                     /locus_tag="VFQ23_21355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9909211.1"
                     /translation="MEINYQETSKDLLTRIDIHEKYGSANIDVWTTDLLKPQAGMKIL
                     DVGCGAGKQSFLFNEYANGGAEITGGDFSEELLDKARAKNKELGTNIDFQFLDFNKPF
                     NFPDNTFDLVTSAFAIYYASDLDFTFGEAHRVLKPGGRLFVSGPLPENKQMFYEIIKE
                     ATNATIPPMPGSSRFKGDIFNTIDRLFSKTELHKFENHLTFPEVAPFIEYVRASLSED
                     RRLWTSMFNGHEEYEALIQSIEKVATRWFERDGKLVMTKVVGGILATK"
     gene            1999..3276
                     /gene="hisS"
                     /locus_tag="VFQ23_21360"
     CDS             1999..3276
                     /gene="hisS"
                     /locus_tag="VFQ23_21360"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET9909212.1"
                     /translation="MAGKVQTVKGTREFYPEQMSLRNFLYEKVRAASQAFGYQEYDGP
                     FIEPIALYAAKSGDELVKKQSFTFEDRGGDLVTLRPELTPSLARMIAAKQNELAYPVR
                     WWSFGPFWRYEQPQKGRTREFFQWNIDMLGVNSPEADAEIIAVGATFLRSVKLDPARA
                     VIYVNNRRLMESEFDALGIPPEKRLDVSNLIDRRAKMDSAKWDANATEMGLSQTQLDE
                     LKVVLSNFDLWKKSEELTRTFTALEALGVKDYIKFDPSIMRGLLYYTGTVYEAFETSG
                     SLKRAIFGGGRYDNLLEDVGGQPLPGVGFAMGDVVIGIVLQEAGLLPEFLPTPAQVLV
                     TVFDEKLSSQSYQLAAELRNAGLNVIVYPETAKLQKQFKFADRMKMKAVLVLGPDEAE
                     KGLVVVKNLLNGEQGPVGREAVIESVRGILNHA"
     gene            3269..3733
                     /locus_tag="VFQ23_21365"
     CDS             3269..3733
                     /locus_tag="VFQ23_21365"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909213.1"
                     /translation="MPEIRPVSLENWQSLIKLKVREDQTGFVASNLYSIAQAQFGDEY
                     EGHWDLHPFGIYDDDEVPVGFLMYGLNFNHPKQQAFIQRLMVDEKFQGKGFGRFAMEK
                     MIEIFRADERIQEVGISYEPHNEGARELYARVGFVETGEMVEEEVVAVLKLK"
     gene            3762..3832
                     /locus_tag="VFQ23_21370"
     tRNA            3762..3832
                     /locus_tag="VFQ23_21370"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3794..3796,aa:His,seq:gtg)
     gene            complement(4048..4407)
                     /locus_tag="VFQ23_21375"
     CDS             complement(4048..4407)
                     /locus_tag="VFQ23_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909214.1"
                     /translation="MYLVLFVLNDPDKLEELLIAWEENDVQGATVLFSTGLGRIHELD
                     NWRDDMPLIPSLRDFYEVPENMNRTVFTVVNDEAQVDAIVAATKKIVGELDEQETGLL
                     LVLPILRAYGMNKRKGS"
     gene            complement(4407..5660)
                     /locus_tag="VFQ23_21380"
     CDS             complement(4407..5660)
                     /locus_tag="VFQ23_21380"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9909215.1"
                     /translation="MTTFLQLAFLLSIILFFAKLAGYISIRLGQPSVLGELLVGILLG
                     PSVIDILHLNFIDPALSHTIAELGELGVLLLMFIAGLELHLDELTRNTRVAAYGGFLG
                     VLVPATLGWGAGRLFGMDNAAAIFLGLTLGATSVSISAQTLMELKVLRSRVGLSLLGA
                     AVFDDILVILSLSIFLAIEAGTGSIGGVLWIVARMIVFLALSVLFGVRVLPWLMRRVA
                     HLPISQGVLTLALVVMLAYGVASELLGGMAAITGAFIAGLMMARSGERERVEHSVHAV
                     AYGLFVPIFFINIGLSVNARSLPVEMLWFTLVIIVAAIIGKWLGAGLGARLGGLTWRE
                     SIQLGAGMVSRGEVGLIVVGVGINQGLVSSNEFSAIVGMVLVTTLVTPPILRALFATN
                     KKSEKTAAEAETMAGSPEQQNEESI"
     gene            complement(5660..6946)
                     /locus_tag="VFQ23_21385"
     CDS             complement(5660..6946)
                     /locus_tag="VFQ23_21385"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET9909216.1"
                     /translation="MIIPAVLSSLIFLTSLVLIFSEKIHRSIVAIAGGVFIVGLGKML
                     GFYSEEAALEAIDFNTLGLLLGMMILVAMLEPTGFFQYIAVWAARVSLGRPTRLFVLL
                     GSITTILSMFLDNVTTVVLIAPVTILICEILGISPLPYLVSEALLSNTGGVATLVGDP
                     PNVLIGSAAGFSFADFLYYSLPVVAVGWVAALLLLRFLFREELMISPPNAQAVMSLNP
                     AQSLDDPKMARRILLVLTVSILFFFIHHLIHISPSFIAVMGAAVALLWVQPDIQELFK
                     RVEWSVLIFFSSLFVMVGGLEAAGVLDAIITVLEGFSNIPPIWFGVSLIWIVAGLSAV
                     VDNIPITIALIPVIRGLGASGMDVDPLWWALAFGAGFGGNGTIIGSTANIIVTTLSEK
                     TRTPITSAIWNRRGLPVMILTCAIASILYILFYEVF"
     gene            complement(6949..7407)
                     /locus_tag="VFQ23_21390"
     CDS             complement(6949..7407)
                     /locus_tag="VFQ23_21390"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9909217.1"
                     /translation="MKISEFMKSNVVSIPESSTVREAAAVFAKAHVGLLPIVDEEKKL
                     VGVVGLRDLLSLVLPDFVNFVADVDFVHDFGAVETSRPSPRILDRSIHSLMKPPITVN
                     EDSGLLRTYALMLQHNLHVMPVVSGDGQLVGIASRVDIGVAIVSTWSLVK"
     gene            complement(7423..>8476)
                     /locus_tag="VFQ23_21395"
     CDS             complement(7423..>8476)
                     /locus_tag="VFQ23_21395"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HET9909218.1"
                     /translation="VVKTKKSILPNPESEEAGQTAISVLVDKWFAENTFSADEFQDLD
                     YLVDLKRERGLSISLALPALNEEETVGNVIQTIKQALLLDAPLLDEIVLIDSNSKDQT
                     REIAQSLDIPVYIHQSVLPESGARSGKGEALWKSLHCTRGDIVIWLDTDIVNIHPRFA
                     YGLIGPLLLRPEIQFIKGYYRRPLKVGDKIQAGGGGRVTELTARPLLNLFYPELSGVI
                     QPLSGEYGGRRAGLEQLSFFSGYGVEIGLLIDVLEKFGLHTIAQVDLQERIHHNQPLE
                     ALSKMSFAIIQAVMRKLESRSGESIMENVNKTMKLIRYEEHRFLLEIEEIAENERPPI
                     LEMAEYRERLNLFAQS"
BASE COUNT         2336 a         2064 c         2006 g         2070 t
ORIGIN      
        1 agagcgttac acacaagacg tccgtgtgct tcgcaatgtg atgcaacagc gctccgcatc
       61 ccagctcgat atcatccggg tgcgcaccga ggaagaggac tcgtttaccg tggaaattca
      121 tattcaagcc ctgtcattgc gaggaggagt aaagcgacga cgaagccgcg aagcgtcctc
      181 gcaccatagg gattgcttcg agctgacgct ctcgcaatga cgctctgtaa tttattcacc
      241 tacaactgcg tacgcccgca cagggaacgt cccgaacgct tttactttca ccggcgcggc
      301 atggaagcgg aatccactcc cgggcaggtt ctccagcccg cacatgtgct cgcaaattgg
      361 aatccgattc cctagaagaa cggtgtgagc agggcgcgtt ccatcggtgg tatcgtcaat
      421 gttataagta tcaattccaa caaatgccgc gccagattct gctaaaaatt cagcagcgtc
      481 tcttgttaga aatggatgtc cctcaaagta ttgatccgtg cgccaatgcc gtgcccagcc
      541 cgtgtggatg agcacggctt tgccttttac atcaatgtct ttaaaagcct ttgcattgat
      601 ggcacgacca aaattctggg catacaccac tgtgctctct aaatctgcca gcgactcaag
      661 gggtagttcg gcaagatcca ttccatctgc aaaccgatga aatggactat ccacgtacgt
      721 tccggtgttg gcgaccattt caatcttgcc aatgttgaat tctgtccctt ccgcgtagtg
      781 cttacgagaa tcctcgcggg agaggaagtc acagataata ggtgcgggga gacccttata
      841 ggtgatcatc ccatgctcca cttcatggct gaggtcaatc agttttcgag tcattcgttt
      901 tttcctgtgt cgttgcgcgg atggcgttga gcccaatgaa gcaatgacaa attggttttt
      961 atttcgtcgc caggattccg cccactacct tcgtcatcac gagttttccg tcccgttcaa
     1021 accagcgtgt agccaccttc tcaatcgact ggatcagggc ttcgtactct tcatgtccat
     1081 taaacatcga agtccataac ctgcggtctt cgcttagaga agcgcgaacg tattctataa
     1141 acggggcgac ttcagggaag gtgaggtggt tttcgaattt atgtaattcc gtctttgaaa
     1201 agagacggtc aattgtattg aagatatcgc ctttgaaacg cgaagacccg ggcataggag
     1261 ggatggtcgc attggttgct tctttgatga tctcgtagaa catctgcttg ttctccggca
     1321 gcggccccga gacgaaaagc cgaccacccg gtttgagaac gcggtgcgcc tcaccaaagg
     1381 tgaagtccag gtccgatgca tagtatatgg cgaacgcact ggtgaccagg tcaaaggtat
     1441 tatcagggaa attaaagggc ttgttaaaat ctaaaaattg gaaatcaata tttgtgccaa
     1501 gttctttatt ctttgcgcgg gccttgtcta aaagttcttc tgagaaatct ccgccggtga
     1561 tttccgcgcc gccatttgcg tattcgttaa agagaaagct ttgctttccc gcaccgcacc
     1621 ccacgtccag gatcttcatc ccggcttgcg gcttaagcaa atcggtcgtc cacacatcaa
     1681 tgttggctga accatatttt tcatgaatgt caatgcgagt gagtaggtct ttgctggttt
     1741 cctgataatt aatttccatt ggtttctcct taagtttccg ttggttgagt aggcggcgct
     1801 tgtccgccgt atcgaaacca acatgttacg aaaatctttc aaattaataa gtggtctcga
     1861 tatacccctc aaaagtattc ggggctactc gaccaccgat ttgaaattat accaaaattg
     1921 cgccaaaatt cagcgactga attttggaag ccgttctgta actcatgata gaatgcgagc
     1981 tactggagtc aagtcaacat ggcaggcaaa gttcaaactg taaaaggcac acgtgagttt
     2041 tatcctgagc agatgtcgtt gcgcaatttt ctgtatgaga aagttcgagc cgcatctcag
     2101 gcatttggat atcaggaata tgatggtccg ttcatcgagc ctatcgcttt atacgcggca
     2161 aaatctggag acgagctcgt caagaaacaa tcgtttactt ttgaggatcg tggtggagat
     2221 ctagtaacac ttcgtccgga gcttacaccc agcttggcgc gcatgatcgc ggcaaagcaa
     2281 aatgaattgg cctatcccgt gcgatggtgg tccttcggcc cattctggcg atatgaacag
     2341 ccacaaaaag ggcggactcg cgaattcttt cagtggaaca ttgacatgtt gggcgtcaac
     2401 tctccggaag cggatgccga gatcatcgcc gtcggggcta cctttctccg ttcggtcaaa
     2461 ctggacccgg cgcgggcggt tatctatgtg aacaatcgtc gcctaatgga gtcggagttt
     2521 gacgcgcttg gaattcctcc cgagaagcgg ctggatgtgt cgaatctgat tgatcgtcgc
     2581 gcgaaaatgg actctgcgaa atgggatgcc aatgcaactg agatgggttt atctcagacc
     2641 cagctggatg aactaaaagt cgttttgagt aattttgacc tttggaaaaa aagcgaggaa
     2701 ttaactcgaa cgtttaccgc gttggaagcg ctgggcgtga aagactatat taaattcgat
     2761 cccagtatta tgcgtgggct gttgtattac acaggcaccg tctacgaggc ttttgagaca
     2821 agtggctctc taaagcgcgc tatctttggc ggcgggcgct atgacaattt actcgaggat
     2881 gtaggcggac agcccttacc gggcgtaggc tttgcgatgg gtgacgtggt gatcgggatc
     2941 gttctccagg aagcaggtct cctgcctgaa tttttgccaa cccctgcaca ggttttggtg
     3001 actgtctttg atgaaaaatt atcttcgcaa tcctatcaat tagctgctga attacgcaat
     3061 gccggattga atgtcattgt gtatcccgaa acagcgaaac tgcaaaaaca attcaagttc
     3121 gccgacagga tgaaaatgaa agcagttttg gtgctggggc ctgatgaagc agaaaaggga
     3181 ttagtagtag tcaagaatct attgaacggc gagcagggtc cggtggggcg tgaggcggtg
     3241 attgagtccg taagaggaat tctaaatcat gcctgagatt cgtccggttt cgctagagaa
     3301 ttggcaatcg ttgatcaaac tcaaagttcg agaagatcaa actggattcg tggcgtcgaa
     3361 tttgtattcc atcgcgcaag cacaatttgg agatgaatac gagggacatt gggatttgca
     3421 tccctttgga atctacgatg acgatgaggt cccggtcggt ttcctgatgt acggattaaa
     3481 ctttaatcac cctaaacagc aggcgtttat tcagcgtttg atggtggatg aaaagtttca
     3541 aggcaagggg ttcgggcgct tcgctatgga gaagatgatc gaaatctttc gcgcggacga
     3601 acgtattcag gaagtcggaa ttagttatga gcctcacaat gaaggcgcca gagagttata
     3661 tgccagggta gggtttgtgg aaacaggaga gatggttgaa gaggaagtgg tggcggtgtt
     3721 gaagttgaag taaatatggt aaaattttga ccgtttccac ggagcctatg gtgtaatggc
     3781 aacacagcac actgtggatg tgctattatg ggttcgaaac ccattaggct cccctaaact
     3841 atttggttgt attttacggc acttattgca tattttatga ctgaatagta catcgttggg
     3901 caccccgtta attcggggtg tctcgatttt aagaccttaa tcttgtcagc aaaagagagg
     3961 gaattttgac ggaacaggga tcaatttatc ttcttacgag tacgtaaaaa tcattgaaac
     4021 gaatcaggga accgaaagct ctgtttttta acttcctttt cttttgttca taccataagc
     4081 acgcaagata ggcagcacta gcaacagccc agtctcctgt tcatcaagtt cacccacaat
     4141 tttcttagtc gctgctacga tcgcatccac ctgtgcttcg tcgtttacga ctgtaaatac
     4201 cgtacgattc atattctcag gcacctcata aaagtcccga agagaaggta tcagcggcat
     4261 atcgtcccgc cagttatcca actcatggat tcgccccaag ccagtactaa aaagaacggt
     4321 ggcgccctgt acatcgttct cctcccatgc tatgagcaac tcctccaact tatcaggatc
     4381 atttagaacg aataagacta aatacattag atggactcct cattttgctg ctcgggggaa
     4441 ccagccatgg tttccgcctc agcagctgtt ttctcactct tcttatttgt tgcgaacaac
     4501 gctcgcagga tcggtggtgt cacaagcgtt gtcaccaaaa ccatccccac gatggctgaa
     4561 aattcatttg agcttaccaa accctggtta atccctacgc ccacaacgat caaaccaact
     4621 tcaccgcgtg aaaccatgcc tgctcccaat tggatagatt ctcgccaagt cagcccgccc
     4681 aacctggcac cgagccccgc gccaagccat tttccgataa tcgcagctac tataattacc
     4741 agcgtaaacc aaagcatttc aacgggtagc gaacgggcat tgacggaaag ccctatgttt
     4801 atgaaaaaga tggggacaaa taggccataa gccacggcgt ggacgctgtg ctctacacgt
     4861 tccctctcac cgctccttgc catcatcagt ccagcaataa aagcgccagt tatggccgcc
     4921 atgccgccca gaagctccga ggctacaccg taggccagca ttacaaccag tgcaagtgtt
     4981 aggactccct ggcttatggg caaatgcgca acccggcgca ttaaccaagg caagacccgt
     5041 acgccgaata gcactgagag agctagaaat acgatcattc ttgccacaat ccataacact
     5101 ccgccaatgc tacccgtacc agcctcgatc gcgagaaaaa tggataggga cagtatgacc
     5161 aaaatgtcat caaagaccgc cgctcctagc agacttaacc ccacacggct acgcaagacc
     5221 ttgagctcca tgagggtttg agctgaaata ctgacacttg tggctcccaa ggttaggccg
     5281 agaaaaattg cagcggcatt atccatgccg aaaagtctac ctgctcccca gccaagcgta
     5341 gctggaacaa ggacccctaa gaaacctccg tatgccgcaa ctcgcgtatt gcgagttagt
     5401 tcatccaagt gaagttccaa accagcgatg aacatcaaca atagtacgcc aagctcacca
     5461 agctccgcga tcgtgtgcga gagggctgga tcaataaagt taagatggag aatatcaatg
     5521 acggatggtc ccaaaagaat tccaactaaa agttctccta aaacagacgg ctgccccagg
     5581 cgaatactaa tgtagccggc aagtttggca aaaaacagaa tgatggaaag aagaaaagca
     5641 agctgtaaga atgtagtcat tagaacactt cgtagaagag tatgtatagg atactggcaa
     5701 ttgcacaggt tagaatcatt acgggaaggc cacggcgatt ccagatagct gaggtaatcg
     5761 gtgttcgcgt tttctctgac aacgtagtta cgatgatgtt ggcagtcgaa ccgataatcg
     5821 tcccattgcc accaaatccg gcaccaaagg caagtgccca ccatagggga tcaacatcca
     5881 tacctgatgc acccaaacct cgaataacag gaataagagc aatggtaata ggaatgttat
     5941 cgacgacagc tgaaagaccg gcaacgatcc aaatcaaact taccccaaac caaattgggg
     6001 gaatatttga aaacccctcc aaaacagtaa ttatagcatc taatacaccg gcggcctcca
     6061 acccgcctac cattacaaag agagacgaaa agaaaataag aacgctccat tcgacccttt
     6121 tgaaaagctc ctgaatatcg ggctgaaccc acaagagggc tacagcggcg cccatgactg
     6181 caataaagga gggactgata tgtattagat gatggataaa gaagaacaaa atggatacag
     6241 tgagcacaag gaggatacga cgtgccattt taggatcatc cagtgactgt gctggattca
     6301 ggctcattac agcctgggcg ttaggtggcg agatcatcaa ttcttcgcgg aacagaaaac
     6361 gtagcagcaa caatgcggct acccatccca cagcaacaac cggaagggaa tagtataaaa
     6421 agtcagcaaa cgaaaaaccg gctgcggagc caatcaacac atttggtggg tcacccacca
     6481 aggttgcaac accgcctgta tttgaaagta aagcctcaga caccagatag ggaagtggac
     6541 taataccaag gatttcgcag atcagaattg ttactggagc aatcaagaca actgtcgtta
     6601 cattgtcaag aaacatggat aggatcgtgg tgatactgcc tagtaaaaca aataagcgtg
     6661 tgggcctacc gagggaaaca cgtgctgccc acacggcaat gtattgaaaa aacccggttg
     6721 gctcaagcat ggcaaccagg atcatcattc ccaacagaag tccgagagta ttgaagtcaa
     6781 ttgcctccag cgcagcttct tcagaataaa agcccagcat ctttcccagt cctacaataa
     6841 atactcctcc tgcaattgcg acgatcgagc gatggatttt ttcagaaaat atcaaaacca
     6901 aactggtcaa aaaaatcagg cttgaaagta cagcaggtat tatcatcgct acttcaccag
     6961 cgaccaggtg gacacgattg ctactccaat atccactcgc gacgctattc ccacaagctg
     7021 accgtcgccc gaaacaacag gcatgacatg cagattgtgt tgcaacatta gcgcatatgt
     7081 acgaagcaag ccgctatctt cattgaccgt tatcgggggc ttcatgagag aatggatcga
     7141 tcgatcaagt atccttgggg agggacgaga tgtttccact gcaccaaaat cgtgaacaaa
     7201 atccacatct gcaacaaagt tgacaaaatc aggaaggacc agggaaagga gatcgcgcag
     7261 accgacgacg ccaacaagtt ttttctcctc atcgacaata ggcaaaagcc ccacatgtgc
     7321 cttcgcgaag accgcggccg cttcccgtac cgttgatgac tccggaattg acacaacatt
     7381 gcttttcata aactcgctaa tcttcattcg tctcctttcc cgttagctct gggcgaataa
     7441 attgagccgt tcacggtatt ccgccatttc gagaatagga ggacgctcat tctctgcaat
     7501 ctcctcgatc tcgagaagaa accggtgctc ttcgtagcga atcaacttca tcgttttgtt
     7561 cacattttcc atgatgcttt cgcctgaacg agactcgagc tttcgcatca cagcttgaat
     7621 gatcgcaaat gacattttgc tcaaagcttc caaaggctgg ttatgatgga tccgttcctg
     7681 caggtcaact tgcgcaatcg tatgcaaacc aaacttttcc aatacgtcaa ttagcagacc
     7741 aatttcgacg ccatagcccg agaaaaaact gagttgttcc aatccagcac gtcgaccgcc
     7801 atattcccca gagaggggct ggattacgcc agagagttcc ggataaaaaa ggttcaataa
     7861 gggacgagcg gttaactcgg ttactctgcc accgcctcca gcctgtattt tgtcaccaac
     7921 tttcaatggc cgtcgatagt atcctttgat gaattgaatt tcgggcctca agagcaaggg
     7981 accgattaat ccatatgcaa aacgggggtg gatattgaca atgtctgtat ccagccaaat
     8041 gacaatatct ccacgtgtgc aatgcagact tttccacagc gcctcacctt taccgctgcg
     8101 tgcaccggat tctggtaaaa cagattgatg gatgtaaaca ggaatatcca aactttgagc
     8161 aatttccctg gtttggtctt ttgagttcga gtcaattaat acgatttcat ccaagagcgg
     8221 cgcgtccagc aacaaagctt gcttgattgt ttggattaca tttcctacgg tctcttcctc
     8281 gttgagcgca gggagtgcaa gacttataga aagtccacgc tcccgcttca agtcgacaag
     8341 gtagtccaaa tcctggaatt cgtctgcact gaaggtgttt tctgcaaacc atttatccac
     8401 gagcaccgat atggcagtct gccctgcttc ctcgctctca ggattgggta gaattgattt
     8461 tttcgtctta acaaca
//
LOCUS       DASTJP010000732         3382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_277417, whole genome shotgun sequence.
ACCESSION   DASTJP010000732 DASTJP010000000
VERSION     DASTJP010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277417"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="VFQ23_21400"
     CDS             complement(<1..1410)
                     /locus_tag="VFQ23_21400"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HET9909219.1"
                     /translation="MTTDFRARIRKAIANEPLQIALDANAERRVKGRINALATLPNPS
                     ERRQQAHAIRADVIEHLDEYLDQFMSNAQANGVNIHRAKNAAEAIQIVLSIVAADGRP
                     LSEKLVAKSKSMVSEEIELNHALEAEGIKVVETDLGEYIVQLRNEKPAHIITPAVHLR
                     RNDVGKLFHEKLGIPYTEDIPTLTNTARKILRNVFLTADVGITGVNFGIAETGGICLV
                     TNEGNGRMVTTMPRIHIALMGMERLVRNLDDLALLLSLLPRSATAQKLSVYTQLVHKP
                     LEHQQRHFIILDNGRTGLRNSPLKESLYCIRCGACLNACPVFRELSGHGYNSTYSGPI
                     GSVISAGFFGNDFVPLAQASSLCGACKEACPVDIDLPKLLVRVRTGLTPQPLTASGES
                     PKGMIRQNEGGGLSTLGRLFMKMYSRIAPYSRLFALSQRLGSLGTHLISPFSKMAWIP
                     SFTGWGYSKDFPKFASKPFR"
     gene            complement(1407..2132)
                     /locus_tag="VFQ23_21405"
     CDS             complement(1407..2132)
                     /locus_tag="VFQ23_21405"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9909220.1"
                     /translation="MTTVQLFISCLVDSFYPETGEAILNILKRLGVDVEFQRDQTCCG
                     QPPFNAGLRSDARPVVEHTIRVFEKTKGDIVIPSGSCAHMLKHGYHELFAEDPVWLPR
                     AEAVASRVYEFTQYLVDKLGVTDLGATWNGTLTFHPSCHALRGMNITYQPRALLEKVK
                     GATLIDLPEAESCCGFGGIFSLEHPELSAEWLKRKIGNLEKTESPTMVVTESGCLMHI
                     AGGLHRQKKRQQIVHIAQVLNSR"
     gene            2206..>3382
                     /gene="glgP"
                     /locus_tag="VFQ23_21410"
     CDS             2206..>3382
                     /gene="glgP"
                     /locus_tag="VFQ23_21410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET9909221.1"
                     /translation="MNTFRTHLPDGFKLPTRLARLGELAYNLWWTWQPEAGRLFGRLD
                     YDLWERLGHNPIRLLREIGRPRLSQASKDKEYREIYDRVFEEFDAYMNETETWTSENH
                     SELSLRTIAYFSMEFGLHETLPIYSGGLGVLAGDHLKESSDLGLPLIGMGFMYAQGYF
                     SQRISEDGWQEALNNMLDFKNLPALPVLHENGERMTVEVEFPDRIVKLGLWEIRVGRI
                     PLYLLDSNFETNSDYDKQLTARLYWSDLDRRVMQEMLLGIGGVRAVRALGYNPSVWHM
                     NEGHAAFLTLERARELVAAGQTFEQALATTRGQNVFTTHTPVPAGNDEFPLWLIEKYL
                     SAIWPQLNLTREQFFDVARHQMPQGEMFGMGVLALRNAEGRNGVSELHGQVARKMWNF
                     "
BASE COUNT          813 a          804 c          902 g          863 t
ORIGIN      
        1 gcggaatggc ttcgatgcaa attttggaaa atccttgctg tagccccagc cggtgaagga
       61 aggaatccat gccatttttg agaagggaga aatgagatga gttccgaggg agccgagtct
      121 ttgtgatagg gcgaaaaggc gcgagtacgg cgcgatgcgg ctatacatct tcatgaaaag
      181 gcggccgagt gtggagagtc cgcctccttc attctggcga atcattcctt tcggactttc
      241 tccggatgca gtgagtggct gaggggtaag tcccgttcgg acccgaacaa gtaatttcgg
      301 taaatctata tccaccgggc aggcctcttt gcacgcaccg cacagagacg atgcctgtgc
      361 aagtggaaca aaatcgtttc cgaagaaacc tgctgaaata accgagccaa tcggtcctga
      421 atacgtgctg ttataaccat gcccactgag ttcgcggaaa accgggcagg cattgagaca
      481 cgcgccgcag cgaatacagt agagtgattc tttaagcggt gagttacgaa gcccggtgcg
      541 cccattatcg aggataataa aatgacgctg ctgatgctcc agcggcttat gcaccaattg
      601 ggtatatacg ctcaattttt gcgccgtggc tgagcgtggc aggagagaca gcagaagcgc
      661 aaggtcgtcc aggttgcgaa caaggcgttc catacccatc aacgcaatat gaatgcgcgg
      721 catggttgtt accattcgac cattgccttc gttggtaacg agacaaatcc cgcccgtttc
      781 cgctattccg aagttgacgc ctgtgattcc cacatccgct gtgagaaaaa catttctcag
      841 aattttacgt gcggtattgg tcaaagtcgg gatatcttct gtgtatggaa tacccagttt
      901 ctcatgaaag agtttaccga cgtcattccg acgcaaatgg acggcgggtg tgataatgtg
      961 cgcaggcttt tcatttcgta gctgaacaat atattcgcca agatcggtct caacgacttt
     1021 gatgccctcc gcctcgagtg cgtgattcag ctcgatctcc tcagagacca tggattttga
     1081 cttggcaact agcttctcgg agaggggtcg tccatccgcc gccacaatgg atagaacgat
     1141 ttggatcgct tctgccgcgt tctttgcgcg atggatattg acgccattcg cctgggcgtt
     1201 ggacataaat tggtccagat attcatcgag atgttcgatc acatctgcac gaatggcatg
     1261 agcctgttgc cgccgctcgc tcggatttgg aagtgtcgcc agcgcgttga tgcgtccctt
     1321 aacgcgccgt tcggcgttgg cgtctaacgc gatttgcagt ggctcgttgg cgatggcctt
     1381 acggatacgt gctctgaagt cagtagtcat cttgaattca atacctgagc gatatggacg
     1441 atttgttgtc tctttttttg ccgatgcagc ccacctgcaa tgtgcatgag acaaccggat
     1501 tctgtgacaa ccatggttgg cgattcggtt ttctccagat ttccaatctt tcgctttaac
     1561 cactccgccg aaagttcagg atgctccaga gaaaagattc caccgaaccc acaacaagac
     1621 tccgcttcgg gcaagtcaat taacgtcgcg cctttgacct tctccagcaa ggcgcgcggc
     1681 tgatatgtaa tattcatgcc tcgtaaggcg tgacaggacg gatggaacgt aagcgtccca
     1741 ttccaggtgg ctcccaaatc tgtgacaccc agtttatcca ccagatattg agtgaactcg
     1801 tacacgcgac tcgccactgc ctccgcgcgc ggcagccaga cgggatcctc tgcaaataat
     1861 tcatgatagc catgcttgag catgtgcgcg caggaaccgg aaggaatgac aatatcccct
     1921 ttagtctttt caaatacacg aatcgtatgt tccacaaccg gacgggcatc tgagcgcaga
     1981 ccggcgttga agggaggttg accgcaacag gtttggtcgc gttggaattc gacatcgacg
     2041 ccaaggcgtt tcaaaatgtt taggatggct tccccggttt caggataaaa agaatcgacg
     2101 agacaggata taaaaagctg tacagtggtc atgagttaat tgtaatatga tttcatcctc
     2161 aaagtcctaa tatcgaattc taaaagtttg gtatgattgc cagccatgaa tacatttcgc
     2221 actcatttac ctgatggttt caaattgcca actcgcctcg cacgccttgg tgagctggcg
     2281 tacaacttat ggtggacctg gcaacccgaa gccgggcgtt tatttggccg gcttgattat
     2341 gatctatggg aacggcttgg ccacaatccg attcgtcttt tgcgcgagat cggtcgcccg
     2401 cgcttaagcc aggcatccaa ggataaagaa tatcgtgaga tctatgatcg cgtatttgaa
     2461 gaattcgatg cctatatgaa tgagactgaa acatggacga gcgagaacca ttctgaatta
     2521 agtttacgca cgatcgcata tttctcgatg gaatttggtt tacatgaaac acttccgatt
     2581 tattcagggg ggctgggtgt gctggctggc gatcacctga aagaatcgag tgatttaggc
     2641 ttgcctttga tcggcatggg tttcatgtat gcgcagggtt atttttctca gcgcatttct
     2701 gaagatggct ggcaggaagc gctcaacaat atgctggatt tcaagaacct gcctgcctta
     2761 ccagttttgc atgaaaacgg tgagcggatg acggtggaag tggaattccc agaccgaatc
     2821 gtgaaattgg gattgtggga aattcgtgtg ggacgtattc ccctgtattt gctggattca
     2881 aatttcgaaa caaattctga ttacgacaaa cagttgacgg cccgattata ttggtcggat
     2941 ctcgatcgcc gcgtaatgca ggaaatgttg ctcggtatcg gtggagtccg tgctgtacgt
     3001 gcgctgggat acaacccctc ggtttggcat atgaatgagg gacatgctgc atttctcact
     3061 cttgaacgcg cgcgggaatt ggttgcagca ggtcagacct ttgagcaggc gctggcaaca
     3121 acacgtgggc agaatgtctt cactacccat acccctgttc cagcaggaaa tgatgagttc
     3181 cctctgtggt tgattgaaaa gtatctttcc gcgatctggc cccagctcaa tttgacgcgc
     3241 gaacagttct ttgatgtggc gcgtcatcag atgccgcagg gtgagatgtt tggcatgggt
     3301 gttctggcat tgcgaaatgc ggaaggacgc aatggcgtct ctgaactgca cggacaggtc
     3361 gcgcgcaaaa tgtggaattt ct
//
LOCUS       DASTJP010000733         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_910446, whole genome shotgun sequence.
ACCESSION   DASTJP010000733 DASTJP010000000
VERSION     DASTJP010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910446"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2255)
                     /gene="cydD"
                     /locus_tag="VFQ23_21415"
     CDS             complement(<1..2255)
                     /gene="cydD"
                     /locus_tag="VFQ23_21415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HET9909222.1"
                     /translation="MHRRLLALSRDSYNALLITILSGFVIGLLTIGQAFFVSRTVDGV
                     FLGGQTLAEVSRYLQFILIIIAGRAILTWLNEVSANALAVKIKSELRERLFSHILNLG
                     PAYTRGQRTGELTTAAVEGIEALDAYYSQYLPQLVMTTLIPISILLVVFPLDLLSGIV
                     MLVTAPLIPLFMMMIGKGAEIVTKRQYQTLSRLSAYFLDSLQGLTTLKLFGQSKAHAQ
                     NIGKVSDQFRDTTLKVLRVTFLSALALELLATLSMAVIAVEIGLRLLYARIEFREAFF
                     LLILAPEFYLPLRMLGARFHAGMAGTTAAKRIFEILDIPISQTTDLTTAREIEITGIE
                     LSNVTYTYPDETRPTLENIDLQIQTGQHVALVGPSGAGKSTLVNLLLGFIQPISGTIT
                     VNREHLISNIELRNSIAWVPQNPHLFHDTISANISLGKPNATQDEVIAAAKAAHLHEL
                     IETLPEKYETVIGEAGARLSSGQAQRLALARAFLKNSPILILDEPTSSLDPETELLLE
                     QSTRRLMQGRTVITVAHRLNTIFRADQIVVLEEGRIVEQGPHHDLLIKNGKYANMINA
                     HQVLGREVEHQKSHVSDKGRSSTIATRPAIKPGLVGSAIQSKIDNRQSLTFFRLLSFL
                     NGSWNWVALSILLGTLTIGSSIALIGTSAWMISTAALHPSIAGLGVSIVGVRFFGISR
                     GVFRYLERLVSHNVTFRLLSRLRVWFYEKLEPLAPARLMEYKSGDLLARVIGDVETLE
                     NFYVRVISPPLT"
     gene            complement(2346..3371)
                     /gene="cydB"
                     /locus_tag="VFQ23_21420"
     CDS             complement(2346..3371)
                     /gene="cydB"
                     /locus_tag="VFQ23_21420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET9909223.1"
                     /translation="MSYEFLQTLWFILIAVLWIGFFFLEGFDFGVGMLLPFLGKKDIE
                     RRAIINTIGSVWDGNEVWLLTAGGATFAAFPHWYATMFSGFYLALFLLVIGLILRGIS
                     FEYRSKDSNPVWRNRFDWMIAIGSFMASLLLGTAFSNLVRGVPIDENMMYTGNLFTLL
                     NPYGLIGGVTMVSVFLLHGANFLTLKLDGELRERAREASKKLYFVAAVAVVVLAISTY
                     IFTDISTKIGINPGIMPIASVVVLLAAIFFINRKMEGWAFISTGVHIILTQVAFFTLM
                     FPRVMISSTDPAYNLTIYNASSSQYTLTVMSIIALIFVPIVLAYQGWTYYMFRKRIIA
                     DKQHLVY"
BASE COUNT          950 a          817 c          877 g          821 t
ORIGIN      
        1 gtcaaaggtg gagaaatcac acgaacatag aagttttcca gcgtctctac atcaccgatc
       61 acgcgcgcta acaaatcgcc gctcttatat tccatcagtc ttgccggagc aagtggctcc
      121 aacttttcat agaaccagac acgcagtctg gagagtaatc gaaacgtgac attatgtgaa
      181 accaaccgtt cgagataacg aaagacaccg cggctaatac caaaaaaacg gacgccaacg
      241 atcgacacac ccagcccggc aatcgatggg tgtaaggctg ccgttgagat catccaggcg
      301 gaggttccga tcaaagcaat gcttgatcca atcgtcagtg ttccgagcaa aatggaaaga
      361 gcgacccagt tccaggagcc gttgaggaag gagagcaggc ggaagaacgt taacgattga
      421 cgattgtcga ttttggattg aatagcagag cccacgagcc ccggcttaat tgcaggtcga
      481 gtagcaattg tcgatgatcg acccttatcc gagacatgtg atttctgatg ttcaacctcc
      541 ctgcctaaaa cttgatgggc gtttatcatg ttggcgtact tgccattttt tataagcagg
      601 tcgtggtgcg gcccctgctc cacaatgcga ccctcttcca gaacaacgat ttgatccgct
      661 ctgaatatcg tgttcaggcg gtgagcgacg gtgattactg tgcgaccctg catgagccgg
      721 cgcgtggatt gctcgaggag caattccgtt tcaggatcca aactcgatgt cggttcatcc
      781 aggattagaa tcggtgagtt cttcaagaag gcccgggcaa gagccaaccg ttgtgcttgt
      841 ccactgctga ggcgggctcc ggcttcccca atgactgttt cgtatttttc aggaagcgtc
      901 tcgatcagtt catgcaaatg ggcggcttta gcagcggcta tgacttcgtc ttgcgttgca
      961 tttggtttac ccaggctaat gttggctgaa attgtgtcat ggaataagtg ggggttttgt
     1021 ggaacccatg cgattgaatt tcgtaattcg atattagaaa tcagatgttc gcggttcacg
     1081 gtaattgtgc ctgatatagg ctgaataaag ccaagtaaaa gatttacgag ggttgacttg
     1141 cccgctccac ttgggcccac cagggcgaca tgttgacccg tttgaatttg tagatcgata
     1201 ttttcgaggg tgggtctggt ttcatctgga tatgtataag taacattcga taattcgata
     1261 cctgtaattt ctatctcacg agctgtagtt aggtcggtcg tttgtgaaat cggaatatcc
     1321 agtatttcaa atattctttt cgctgccgtc gtaccagcca tacctgcatg aaagcgcgcg
     1381 cccagcatgc ggagtggtaa ataaaactca ggggccagga tgagcaggaa gaatgcctca
     1441 cgaaattcaa ttctcgcgta gagtaatctc aacccgatct ctaccgcaat gacggccatg
     1501 ctcaaggtgg caagcaattc caatgcgagc gcagaaagaa atgtgactcg taacactttg
     1561 agcgtcgtat cgcggaattg gtcgctgact ttgccaatgt tttgtgcgtg tgccttggac
     1621 tgcccaaaga gcttcagagt tgttaaaccc tgcaggctat ccagaaagta ggctgaaagg
     1681 cgggagagag tctgatattg gcgttttgta acgatctctg cacctttacc aatcatcatc
     1741 ataaataaag gaatcagtgg cgcggtcacc aacattacaa tgccagataa caaatctaag
     1801 ggaaatacca ccaacagaat gctgattggt atcaaggttg tcatcaccag ttgaggtaaa
     1861 tactggctat agtacgcatc caatgcttcg atgccttcga ccgcggcggt ggtcagctcg
     1921 ccggtacgtt ggccacgcgt gtaggctgga ccgagattca gaatatggct gaagagtctc
     1981 tcgcgcaact ctgactttat ttttacagcg agcgcattcg cgctgacctc gtttagccag
     2041 gtcagaatcg cccgccctgc gatgatgatt aggatgaact gaagatacct tgatacttcc
     2101 gcgagggttt gtcctcccag aaacactcca tcaacagttc tgctgacgaa gaaggcttgg
     2161 ccaatcgtca acagaccgat tacaaatccg gaaaggatag ttatgaggag tgcattgtaa
     2221 gagtcgcgag agagagcaag aagtcgacgg tgcattatac gtatcgggta tccaggtcca
     2281 cattaaaagc attggaaggc tgaggagtat ctcgccaacc ttccaatgac ggagggacta
     2341 ttattctaat aaacgagatg ttgcttatcg gcgatgatcc gtttacggaa catgtaatac
     2401 gtccagcctt gataggcaag cacaatgggc acgaagatca aggcgataat ggacatgacc
     2461 gtcaacgtat attgtgatga ggatgcgttg tagattgtga ggttataggc aggatcggta
     2521 ctggagatca tcacgcgcgg gaacatcaaa gtgaaaaaag cgacctgcgt caaaatgatg
     2581 tgcacacccg tggagataaa tgcccaaccc tccatcttgc gattgatgaa gaaaatcgca
     2641 gctagcagga caaccactga tgctatcggc ataatgcccg ggttgatacc gatcttggtt
     2701 gaaatatcgg tgaagatgta ggtcgaaatc gccagcacca caactgcaac agcggctacg
     2761 aaatacagct ttttggatgc ttctcgcgcg cgttcacgaa gttcgccatc cagttttagg
     2821 gtgaggaagt ttgcgccatg cagcagaaat acagagacca tggtaacccc accgatcaaa
     2881 ccgtatggat taagcagggt aaacagatta cctgtgtaca tcatattttc atcgattggc
     2941 accccgcgaa cgagattcga aaatgcagtc cccagcaaca gcgacgccat gaaggaaccg
     3001 atagcgatca tccaatcgaa gcgattgcgc caaacagggt ttgaatcctt cgaccggtat
     3061 tcgaatgaga tgccgcgcag gatcagtccg atcaccagga ggaacaatgc aagatagaac
     3121 ccgctgaaca tggtggcata ccagtgcggg aaggcggcaa aggttgctcc gccagcggtc
     3181 aacaaccaga cctcatttcc atcccacacc gatccaatcg tgttgatgat agcgcggcgt
     3241 tcaatatcct ttttgccgag gaaaggcagg agcattccca cgccaaagtc aaaaccttcc
     3301 aggaagaaga agccaatcca caatacggca atcagaataa accaaagagt ttgcaaaaat
     3361 tcgtacgaca tgatttgtct ccttacttag ccctgggcac caattggaag tgcttcagac
     3421 aaactatctt cttctacatc aacggactcg tgcaaggtgg catct
//
LOCUS       DASTJP010000734         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_170798, whole genome shotgun sequence.
ACCESSION   DASTJP010000734 DASTJP010000000
VERSION     DASTJP010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170798"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="VFQ23_21425"
     CDS             complement(<1..1857)
                     /locus_tag="VFQ23_21425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET9909224.1"
                     /translation="MKTTLALPKSPKVQFVGITRRFKEFSLIFRDPVLFMSLLFSGIF
                     IFVFVVLPILRTISGGFFSEEGTLDFTYFGRYFDSFYGPGLRQAFLDTMIMGLLTAVF
                     GTLVGFIFAYASVRCNPPAKNLLHWLALLPTVSPPFALALSVILLFGRSGLITKKVLG
                     ITYVPGMNDIYGLDGLVLVQTITFFSVAYLILRAMLERLNPSLEEAAASLRASRWHIF
                     RTVTLPLLIPGIAGSFLLLFVESLADLGNPLFIAGNRTVLSAQIFLAVIGEYDYQKAS
                     TLSLILIIPTLVLFMIQRYYVNRRSYISVTGKPSGAQITEKDPIIRWGFNIAAYLICA
                     FVILLYATIFYGSFSFTWGVDFTPTLRHWSMVVTRGVEAILDTTFLSALATPFAALLG
                     MIIAWLVVRKNFSGKEVLDFTSNLGGAVPGTILGIGFVLVFNKPPLALAAVIYALLAM
                     FFVLVVGKGARERLIILLVGTAIGVLLTRMAAVQAYYLLGGLYLVLAAILFISNRNTF
                     MALCSASLGIYVMSTNWATTIVQPIADYSRSIERGFWSNAIFQFSDHIKVLLQPPPSL
                     LAIFLVFAGILLVQGLKQPLPRVAGGILALTIPCTMSFIGIPFALVGGAYIIM"
BASE COUNT          637 a          512 c          493 g          454 t
ORIGIN      
        1 cataataatg taggcacctc caaccagcgc aaacggaatc cctatgaaag acatggtgca
       61 gggaatggtc aatgccaaaa taccccccgc tactcttgga agcggctgct tcaatccctg
      121 aaccaaaagg atgcctgcaa agacgagaaa aattgccagg agggaggggg gcggttgtaa
      181 gagtacttta atatggtccg aaaattggaa aattgcattg ctccagaaac cgcgctcgat
      241 gctccgactg tagtcagcga tgggttggac gatcgtggtc gcccaattcg tactcatcac
      301 atatatgcct aaagatgcag agcacagtgc cataaatgtg tttctattgc tgatgaaaag
      361 gatcgctgcc agcaccagat atagaccgcc cagcaaataa taagcctgta cagccgccat
      421 tcgggtcagc agtacaccga tggcggttcc caccagcaaa atgatcagac gttcacgagc
      481 acccttgcca acgaccaaaa caaaaaacat cgccagtagt gcataaatca cagctgccag
      541 tgcaagcggc ggcttattga aaaccaaaac aaatccaatt cccaggatcg tcccaggtac
      601 ggctccgcca aggtttgagg tgaagtcaag gacttctttc ccactaaagt ttttcctcac
      661 caccagccag gcaatgatca tacctagaag ggcagcgaag ggagttgcga gcgcgctcaa
      721 gaacgtagta tccaggatcg cttccactcc acgcgtaacc accatcgacc aatgccttaa
      781 cgtaggtgtg aaatcaactc cccatgtaaa gctgaaggaa ccataaaaaa tcgtcgcata
      841 aagcaaaatc acaaacgcgc agatcaaata agcggcaata ttaaatcccc agcgaatgat
      901 aggatccttc tccgtgattt gcgcgccgga tggtttgccc gtcacagaaa tgtaggagcg
      961 tctgttgacg taatatctct gaatcatgaa taagaccaat gttgggatga tcaagatcaa
     1021 cgataatgtc gaggcttttt ggtaatcata ttcgccaatc actgccagga agatttgagc
     1081 cgaaagcaca gtgcgatttc ctgcaataaa aagtgggttt cccaggtcag ccaaggattc
     1141 gacaaataat agaagaaacg aacctgcaat tccgggaatc agaagcggaa gggtcacagt
     1201 tcgaaagatg tgccatcgac ttgcacgcag gctggcagct gcctcttcca gggatgggtt
     1261 taggcgctca agcatcgcgc gcaggatcaa gtaagcaact gagaaaaatg tgatcgtctg
     1321 aacgagaacc aatccatcca atccataaat gtcgttcatc cctgggacgt aagtgatccc
     1381 aagaactttt ttcgttatca atccactccg cccaaataag agaatcacac tcaaggctaa
     1441 tgcgaagggt ggagaaacgg tgggcaacag agccaaccag tgcagcaggt tttttgcagg
     1501 cgggttgcag cgtacgcttg cgtaggcaaa aataaatccc accaacgttc caaaaactgc
     1561 tgtcaatagt cccatgatca tggtatccag aaacgcctgg cgcaacccgg gaccataaaa
     1621 actgtcgaag tatcgcccaa agtaagtaaa gtcgagagtt ccttcttcgg agaaaaatcc
     1681 acctgaaatc gttctcagga ttggcaatac gacaaacaca aagataaaaa ttccagagaa
     1741 cagcaggctc atgaagagta ctggatctct gaaaattaac gagaattctt taaaacggcg
     1801 ggtgatgccc acgaactgca cttttggcga tttgggtaag gcaagtgtgg tcttcattct
     1861 cctccgatat gcaattcagg gtggcactac ggtgccaccc tgaatttgta aacaaacgac
     1921 tattctttta gattgtctgc gcctgcgatt tcgttcgtaa atttatcgac aaactcctgc
     1981 ttatgttcgc ccgcccagat gaagtcatag tcaatcaggt tgacttcgag caattcagga
     2041 tgtgaaagtt cgacaccgct gactgtcggt gcctgatagg cagcatacgt ggggcc
//
LOCUS       DASTJP010000735         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_910668, whole genome shotgun sequence.
ACCESSION   DASTJP010000735 DASTJP010000000
VERSION     DASTJP010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910668"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1642)
                     /locus_tag="VFQ23_21430"
     CDS             complement(11..1642)
                     /locus_tag="VFQ23_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909225.1"
                     /translation="MQNLTRRDLLWLLPLALVLGAILSSVQPGSWLIGFLSFSFLFLL
                     SFSVLTLSVKWANAGKTLAWIVALAFALRFIVGLTLYFGLPVYGHDDVDDQAGFVFTD
                     AHRRDDQAWDLASSDRPILVAFTDRFAYDQYGGLLAFSAFIYRYFSPDVHRPLMLVLI
                     SAFMGALGVPFLWKAVSQVFGERVAWASAWIFALYPESILLGASAMREPYLLTFSAVA
                     LWGFVEWHGTSSAHSPSLRAPLSAMVFGLLGMLLISPAIALATLIIFAGWMFFTSERS
                     SISWRTVLIFGLIFIVGLLFLSTSLNASGQFDSSSPLSVINDWLRSAVKWNVYQIADE
                     SGWVQKIFRLREENEAPAWIRLPFVAIYGLLQPVLPATILHPTKLIWMIIGFLRGLGW
                     YALIPLLILSFGAAAGSSSNKTRNLILWLALFTWTWILLASIRGGGDQWDNPRYRTLL
                     FLWQAILAGYVWVWWRETRNAWLPRILVMEGVFLLIFTQWYASRYYHIGGQLPFSVMV
                     AMIIGLWGLIIAVGWWQDRGVAKRVSENRLRHREH"
     gene            1725..2045
                     /locus_tag="VFQ23_21435"
     CDS             1725..2045
                     /locus_tag="VFQ23_21435"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9909226.1"
                     /translation="MSPKIQRLLADLKKGLTDLYGTRLKAVYLFGSYARGDYNNNSDL
                     DVMIVLDNYISYWDELVYSAELASNLSLEYNVTISRMIMTEDQWKKGDLPILRNIRAE
                     GLPA"
     gene            2042..2422
                     /locus_tag="VFQ23_21440"
     CDS             2042..2422
                     /locus_tag="VFQ23_21440"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HET9909227.1"
                     /translation="MKETTKQLLARAVDTIEAADILLTNEKTDIAAGRAYYAMFYVAE
                     ALLNEKGFQFGKHGNVIAAYGQHFAKTKELDPKFHHWLLTSFDQRQIGDYAFDPDIQA
                     SVVLQMIEQAQEFLEAAKAYLVED"
     gene            2577..3407
                     /locus_tag="VFQ23_21445"
     CDS             2577..3407
                     /locus_tag="VFQ23_21445"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9909228.1"
                     /translation="MLKEQIALVTGASRGVGKGIAQALGAAGATVYVTGRTERANDAT
                     VPLPGTIHETAALVTQAGGTGIAIRCDHRDDDQVRAVFDQISNTHGRLDLLVNNAWEG
                     YQAKQRSRKSGFHTSFWKLPPTFWDTMFTVGVRSHYVAGVYGAAIMAERQRGLIIQIS
                     STTGDGYSDNVAYGVSKAAVHRLTMDMAHELKPHRVAVIALCPDIVATEMIMARRKNQ
                     ALEKWMESPLFVGRAVVALATDPQIMAKTGSVLRTRALAQEYGFTDIGGHEPQWKPEQ
                     "
     gene            complement(3422..>4485)
                     /locus_tag="VFQ23_21450"
     CDS             complement(3422..>4485)
                     /locus_tag="VFQ23_21450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9909229.1"
                     /translation="LGHNLTLLVPGNRTREAQSGIVDLKSHYGLQAEFPIEWLASTNR
                     RLFTWQAVRRARELKPDLLYTWFPQSAVFGLLYKLPVVFEIHIQPTGFFGPVWHQAFA
                     SLPGRKRLVSITHALVNLLERDYNMRLKPEEVIIAPNGVDLERFASLPDPVTARRQLS
                     LREASTVMCTGHLYAGRGADLFFSLAKSIPHAHFVWVGGRAEDIESWKPRAESQNISN
                     ITFTGFISNSDLPLYQAAADILLMPYSRSIMGSSGTADSASVASPMKMFEYMAAGRAI
                     ATSDLPVIREVLNERNAVFCEPDEVGSWKLAIEALLKDEQRRGELGDQARLDVESYTW
                     VARATRILKEFPSSKSIKA"
BASE COUNT         1278 a         1110 c         1122 g          975 t
ORIGIN      
        1 gtcaaagggc tcagtgctct ctgtggcgaa gtcgattttc actcactctc ttagccactc
       61 cacgatcctg ccaccagcct acagcaataa tcagccccca taagcctata atcatggcga
      121 ccatgacaga aaatggcagc tgcccaccga tgtgataata ccgactggca taccattggg
      181 tgaagatcaa tagaaagacc ccttccatca ctaaaatccg tggcagccac gcgttccgtg
      241 tttctctcca ccagacccaa acatacccgg caaggatggc ttgccataag aacaggagcg
      301 tccggtagcg tggattatcc cactggtcac cgccaccgcg tatggaagca agcagaatcc
      361 aagtccatgt gaagagcgcc aaccagagga ttagatttcg cgtcttattg gaactcgatc
      421 cggccgcggc gccaaaagag aggatcaaca gcggaattag cgcataccaa cccaggccgc
      481 gcagaaatcc gatgatcatc cagatcagtt ttgttgggtg aaggatcgtc gcaggcagga
      541 ctggctgaag aagaccataa atcgcgacaa acggcagccg aatccatgca ggagcttcat
      601 tttcttcacg taaacgaaag atcttttgta cccagccgga ttcatcggcg atttgataca
      661 cattccattt gaccgcagag cgcagccaat cattgatcac acttaatgga gaggatgaat
      721 caaattgacc ggaggcgttg agagaagttg aaagaaaaag taacccgact ataaaaatga
      781 gaccaaagat aagaactgtt ctccatgaga tactgctgcg ttcactggtg aagaacatcc
      841 agccggcgaa gatgataagc gtagcaagcg caatggcagg ggagatcagc agcatgccta
      901 gcaggccaaa gaccatcgcg ctgagcggag cacgaagcga aggggagtgc gcagacgaag
      961 tgccgtgcca ttctacaaac ccccataatg ccacggcact gaacgtgagc aaatagggtt
     1021 cacgcatggc agacgcgcca agcaaaatac tttcaggata caaagcaaag atccatgccg
     1081 acgcccacgc aaccctttcg ccaaatacct gactcactgc tttccataaa aatggaactc
     1141 ccaacgcgcc catgaaggct gaaatcagca ccagcatcag cgggcgatgc acgtccggtg
     1201 aaaagtatcg ataaatgaaa gcgctaaatg ccaataaccc accatactgg tcgtaagcaa
     1261 atcgatctgt aaacgcgaca aggatcgggc ggtcagaact ggcaagatcc caggcctggt
     1321 catcgcggcg atgcgcgtcg gtgaagacga agcctgcctg gtcatctaca tcatcatgtc
     1381 cgtagacagg caagccaaaa taaagtgtta agccaacaat aaagcgtaag gcaaaggcaa
     1441 gagctacaat ccatgcgaga gtttttcccg cattcgccca tttgacagaa agtgtgagta
     1501 cagagaatga aagaagaaag agaaaagaga acgaaagaaa gccaatcagc cagctgccag
     1561 gttgaacaga ggatagtatt gctcccaaga cgagggcaag aggaagcaac cagagaagat
     1621 cgcgtcgagt tagattctgc atattcccac ttttaccatg aaaaccaccc tgctcgctgg
     1681 atgaagattg atatgttagg tttataatac actgtgaggc atccatgtca ccaaaaatcc
     1741 aacgcctgtt agccgattta aagaagggat tgaccgatct ttacggcact cgcctgaaag
     1801 ccgtatattt attcggctca tacgctcgcg gggactacaa caataattcc gacctggacg
     1861 tgatgattgt gttggacaac tatataagtt attgggatga gcttgtttat tcggcagaac
     1921 tggcaagcaa cctgtcactt gaatacaatg taacaatcag ccgaatgatt atgacggaag
     1981 accagtggaa gaagggtgac ttacctatct tgaggaatat ccgcgctgag ggactacctg
     2041 catgaaagaa actaccaaac aattgcttgc ccgcgcagtg gacacaattg aagcagcaga
     2101 tattttgctc accaatgaaa agaccgacat cgctgccgga cgcgcctatt atgcaatgtt
     2161 ttacgttgct gaggcactac ttaacgaaaa agggttccag tttggcaaac acggaaacgt
     2221 catcgcggca tatggacagc attttgcaaa gacaaaagag ctggatccaa aatttcacca
     2281 ttggctgctt acttcattcg accagaggca gattggggat tatgcctttg accctgatat
     2341 tcaagctagt gtcgtccttc aaatgattga acaggctcag gaatttctgg aagctgccaa
     2401 agcatatttg gttgaggact agagattcca attcctatca agagcgaaag agggaaaatc
     2461 caaagaagag cacttttgtt agtttcatct cgtttgattc tacaccagtt acccactgcc
     2521 actttctgga tataattctg gacctacttt atacataata taaggaagcc atacaaatgt
     2581 tgaaagagca gatcgcgctg gtaacgggtg caagtcgagg agtcggcaaa ggtattgcac
     2641 aggcgttggg cgcggcgggt gccacagtct atgtgactgg gcggactgag cgcgcaaacg
     2701 atgccaccgt gccactccct ggaaccattc atgagacggc cgcactcgtc actcaagcag
     2761 gcgggaccgg aattgcaata cgatgcgatc atcgggatga tgaccaggtt cgagcggtgt
     2821 tcgaccaaat tagtaacacc catggtcgtc ttgatctctt agtcaataat gcctgggagg
     2881 gctaccaggc gaaacaacgc agcaggaaaa gtggctttca tacatcgttc tggaaacttc
     2941 ccccaacctt ctgggatacg atgtttactg ttggcgttcg ctctcactat gtagccggcg
     3001 tctatggtgc tgcgattatg gcagaacggc aaagaggcct catcatccag atttcgtcaa
     3061 ccactgggga tggatatagt gacaatgtag cctacggggt tagtaaggca gcggtgcatc
     3121 ggctcacaat ggacatggcg catgagctga agcctcatcg cgtcgcagtg atcgccttgt
     3181 gccccgatat cgtggcgacc gaaatgatca tggcgcgccg caagaatcag gctcttgaaa
     3241 aatggatgga gtcccctctt ttcgtcggac gcgcagttgt cgccttagct actgatccac
     3301 aaattatggc gaaaacaggt agcgttctca ggacacgagc actggcgcag gaatatggat
     3361 ttacggatat aggtggacat gaacctcaat ggaagccaga acagtaagct aactgttgtg
     3421 gttatgcttt gatactctta cttgatggaa actctttcag aattctagtt gccctcgcca
     3481 cccaggtata actctccaca tcaagtcgtg cttgatcacc caactcacct cggcgttgtt
     3541 catcctttag caacgcctca atggccaatt tccagcttcc aacctcatct ggttcacaaa
     3601 aaaccgcatt cctctcattc aacacttccc gaattacagg caaatctgag gtagcaatcg
     3661 cccgccccgc tgccatgtat tcgaacatct tcatggggct ggcaacactg gcggagtctg
     3721 cggttccact agagcccata atggaacggc tgtatggcat aagcaatata tctgctgcgg
     3781 cttgataaag tggcagatca ctattggaaa taaatcccgt gaaagtgata ttggatatat
     3841 tttgagattc tgcgcgcggc ttccaacttt ctatatcttc agcgcgacca cccacccaca
     3901 cgaaatgcgc atgcgggatg gattttgcca gagaaaaaaa caggtccgca ccgcgacctg
     3961 cgtagagatg ccctgtgcac atcacagtcg aggcttcgcg aaggctaagt tgacggcgcg
     4021 cagtgacagg gtctggcaag gaggcgaagc gttccaaatc cacaccgttt ggcgcgatga
     4081 tcacttcctc cggtttgagt cgcatgttgt agtcgcgttc gaggaggttg accagtgcat
     4141 gagtaatcga gacgagtcgt ttcctgccag gtaacgaggc aaacgcttga tgccacacag
     4201 gaccgaaaaa acctgtaggc tggatgtgga tctcgaaaac aacaggcagc ttgtagagca
     4261 agccaaagac tgcagattga gggaaccagg tatatagcag atcaggtttg agttcacgtg
     4321 cccgtctcac agcctgccat gtgaacaacc gacgatttgt agaagcaagc cattcgatgg
     4381 gaaactcagc ttgaagacca tagtgcgatt ttagatctac gattcccgac tgcgcttcgc
     4441 gcgtgcgatt gccgggcacg aggagcgtga gattgtgtcc tagtt
//
LOCUS       DASTJP010000736         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1021999, whole genome shotgun sequence.
ACCESSION   DASTJP010000736 DASTJP010000000
VERSION     DASTJP010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021999"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3087)
                     /locus_tag="VFQ23_21455"
     CDS             complement(<1..>3087)
                     /locus_tag="VFQ23_21455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9909230.1"
                     /translation="DTNPSLLYKPSQELLGKTLHEVFPKDQADFFLHHLRQVIDTQES
                     VSFEYSIPIGDTKMWFYATVSPLPEHKTLMVARDITDQKEAQAQVLASEQLFRALVEN
                     SPDFIARYDREFRRIYVNPAIQKLFGDQVENVLGKTPADQSPVYAPQIYINHLRQVIE
                     TGTESVTEMPFLTAKGEMHWGHMRFVPEFGPEGRVESVLAIGRDIHEIKENERRFRML
                     AENFPDFVMRFDSEGRYIYVNPAFEKAVGMPAESIIGKTLQELPQRIKTEQNRARLDS
                     IRRALDEGISNESEVRWDTEKGERIFEDRYVPERDATGNVVTVLCIARDVTEQKRAEE
                     ELQKTNDLLRAIIEAAPTAIIGLDLEGKVQNVWNPAAEKMLGWSAEEALGKFLPSVSV
                     DKEEEFQKFRQWVRSGKSMNGVEVQRRRRDGTPIDYSIYASPLHDTQGQTIGNIAVLV
                     DITERKRVEQALRESEERYRLIAENTADTIAVFDLNLRPTYISPSIIKLRGYSVQEAL
                     AQSLDQMLTPESLQQAQKVLADQMALEASGEANLSRTSLFELEEYCKDGSVIWVELAA
                     SFLRDENSQPTGVLTVTRDITERKRAEDDLRASETRFRTLVDHAADAFFLYDEQGTIL
                     DVNRQACESLGYSRHELIGMTPYEIDSMLDHSFIGQLESRLDAGDTVAFETRHRRKDG
                     SVFPVEVRGRPFWHGERRFSVALARDITERKQAEEQRKEHVRFLESMDKVNRAMQSAN
                     DIEWVMNDVLDLVLSIFDCDRAYLGYPCDPETPSWSLRAESTRPEYPGLLALGIDEVP
                     IDPEGTEIFRILLNTDGPVTFGPGNSHPLSANATNRFGLQSFMSMAFFPKVGKPWQFG
                     IHQCSYTRVWTPEDERLFHEIGRRLSDVLTVLLTYHHLEESEERYRQLVNLSPDGISI
                     HRDGRVQFVNPAMVRMSGATSADQLIGKSVLDGIHPDFRAAARNRMHEMESSGQPLPF
                     IEEKLLRLDGSSIDVELAAVPYNINNTRYILIVARDITERKRAEEALRESEERLRQI"
BASE COUNT          690 a          836 c          771 g          790 t
ORIGIN      
        1 aatttgacga agtctttctt cgctctcccg caaggcttct tccgcccgct tgcgttccgt
       61 gatatcacgc gccacaatca gaatgtaacg ggtgttattg atattgtaag ggacagcggc
      121 aagttccaca tcgatggagc tcccgtcaag ccgaagtagt ttttcctcga tgaacgggag
      181 gggttgtccg ctactctcca tctcatgcat acggtttcgt gcggccgcgc gaaaatctgg
      241 atgaatgcca tcgagcacag atttaccgat gagctgatcg gcacttgttg cgccagacat
      301 gcgcaccatc gccggattta cgaactgaac ccgtccatca cgatggattg aaatcccgtc
      361 tggagacagg ttgacaagct ggcgatagcg ttcttcactc tcttcaaggt ggtgataggt
      421 aagcaaaacg gtcagaacgt cggagagacg tctgccaatt tcatggaaaa gcctctcatc
      481 ttccggtgtc caaacccgtg tataggaaca ttgatggatc ccaaactgcc agggcttgcc
      541 cacctttggg aaaaatgcca tggacataaa agactgtaga ccaaaacgat tagttgcatt
      601 tgctgacaat ggatgtgaat ttccggggcc aaatgttaca ggaccgtcag tgtttaacaa
      661 tatgcgaaat atctctgtgc cttccggatc tatgggtacc tcatcaatcc ccagggcgag
      721 gagtcccggg tattctggcc gggtgctttc cgcacgcaga ctccatgatg gagtctctgg
      781 atcacaggga tagcctagat atgcccgatc gcaatcaaaa atggacagga ccaggtcaag
      841 aacatcgttc atgacccact cgatgtcatt cgcgctctgc atagctcgat ttactttatc
      901 catgctttcc aaaaaccgta catgctcctt gcgctgctcc tccgcctgtt tgcgttcggt
      961 gatgtcccgg gccagggcga ccgaaaaccg gcgctcgccg tgccagaaag ggcggccccg
     1021 cacctcaact ggaaacacgc ttccatcctt ccggcggtgc cgggtttcga aggcaaccgt
     1081 gtctccggca tccaatcgtg attccagctg accaataaag gagtgatcaa gcatcgaatc
     1141 gatttcatag ggtgtcatcc cgatcagttc atggcgactg tagcccaggc tctcgcaagc
     1201 ctggcggttc acgtccagga tagttccctg ctcgtcgtac aagaagaagg cgtcagccgc
     1261 gtgatctacc aaagtacgga agcgtgtctc gctggcacgg aggtcgtcct ccgcccgctt
     1321 gcgctccgtg atatcccggg tgactgtcag taccccggtt ggttgggaat tctcgtctct
     1381 aagaaatgat gccgcgagct caacccaaat cacagaacca tctttgcaat actcctcaag
     1441 ttcaaacagg gaggttcttg acagattagc ttctccactt gcctctaaag ccatttgatc
     1501 ggcaagtacc ttctgtgcct gctgcaagga ttcaggcgtc aacatttgat ccagagactg
     1561 tgccagtgct tcctgtactg aatatccacg cagctttatg attgaaggac tgatataggt
     1621 tggacgaagg ttcagatcga acacggctat tgtatcggca gtattttcgg caatgagacg
     1681 gtagcgttcc tcgctctcgc ggagtgcctg ctccacccgc ttgcgttcgg tgatgtctac
     1741 cagcacagcg atattgccaa tggtttgtcc ttgcgtgtca tgcaggggag aggcatagat
     1801 gctgtaatca atcggtgtac cgtcacgtct tcgcctttgg acctcaacac cattcatgct
     1861 ttttccactg cgcacccatt ggcggaattt ctggaactct tcttctttat ccacagaaac
     1921 gctggggagg aacttgccca gggcttcttc ggcgctccac ccaagcattt tttcagccgc
     1981 cggattccag acattttgta cttttccctc caggtccaga ccgataatgg cagttggagc
     2041 cgcctcgata atcgcgcgca ggagatcgtt ggtcttttgg agttcttctt ccgctcgctt
     2101 ctgttcggtg acatcgcggg caatacagag gacggtgacc acgtttccgg ttgcatctct
     2161 ctccggcaca tagcgatcct cgaaaatgcg ctcgcctttc tctgtatccc aacgcacctc
     2221 ggattcattg gagatccctt cgtccaacgc acggcggatc gagtccaggc gcgccctgtt
     2281 ctgttccgtt ttgatgcgtt gaggcaattc ctgcagggtt ttgccaataa tcgattcggc
     2341 tggcatgccg actgcttttt cgaacgcggg attgacgtaa atataccgac cctcgctatc
     2401 gaaccgcata acaaagtcgg ggaagttttc tgccagcatt ctgaagcgcc gctcgttttc
     2461 cttgatctcg tggatatcgc gcccaatggc cagcacagac tccaccctgc cctctggccc
     2521 aaattccgga acaaacctca tgtgacccca gtgcatctct cctttagctg tgaggaaagg
     2581 catctccgta acgctttccg tacctgtctc gatgacctgc cttagatgat taatgtaaat
     2641 ttgcggggcg taaaccggcg actggtcagc cggtgtcttg ccaagcacat tctccacctg
     2701 gtctccgaat agcttctgga tagcagggtt aacgtagatg cggcgaaatt cccgatcata
     2761 gcgggcaata aagtcaggcg agttctccac aagggcacgg aaaagttgtt cgctggcgag
     2821 aacttgtgct tgcgcttcct tttgatcagt aatatctctg gcaaccatca gtgtcttgtg
     2881 ctcgggcagc ggagacacgg tagcataaaa ccacattttc gtatcaccga tcggtatact
     2941 gtattcaaaa ctgaccgatt cctgggtgtc aataacctgc ctgagatgat ggagaaaaaa
     3001 gtcggcctga tctttaggga aaacttcgtg taatgttttt cctaatagtt cttgtgatgg
     3061 tttatataaa agtgacggat tcgtgtc
//
LOCUS       DASTJP010000737         4983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_699154, whole genome shotgun sequence.
ACCESSION   DASTJP010000737 DASTJP010000000
VERSION     DASTJP010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699154"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VFQ23_21460"
     CDS             complement(<1..1096)
                     /locus_tag="VFQ23_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909231.1"
                     /translation="MNQAQNASRSFWLTILIFTFMVNLTMLRLTYLRLIEIGANLPRA
                     TWSGMLVICVAVMLICLWLIFSIVRSDTLPFSLISRLSHVKFDNLLWRVLGILLFLGI
                     LILIPYIKFRFELGQAVKETTRDPILVLYLYYWLCWWALLLGMWALKVGFGTTWQIGF
                     ASALILLGVAYEILIRFNLVTSYPLSLGWSEGSRYYYASLYFSNWIYGESFPLSTLHP
                     TRYLLQSVPFLIPDLGLFAHRFWQFFLWIGLTAAGALAIAKRAFSSQEKYGQWLTAGW
                     LFLFLLRVGVYYHLEVMVILPLLFVSAKHPWRSLIAVIAASLWAGISRVNWFPMPAMI
                     AIALYLLETPFASVINNHSFKGIVKYLSQPV"
     gene            complement(1103..2029)
                     /locus_tag="VFQ23_21465"
     CDS             complement(1103..2029)
                     /locus_tag="VFQ23_21465"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9909232.1"
                     /translation="MPKGSWWLRNRQRLLQLVGTLLAVVMIILLVRNDEWGEIVAAMQ
                     KITILDLILVTILFAISRLAVAWRWHMLLQSGGVHIPLKKSLALTFTGLFASNFLPTT
                     IGGDVVRLAGAMQMGFDRAVCLASIAADRLIGMLGMGMTVPIGLSYSWNMIQLGVASS
                     SLVGVLQKPVAFIKRTASIFSIWLKKPAFLLGSLAFTWIHMLCLFGSIYVFVNDLGSH
                     ISFWMIAGLWSLTYFITLIPISINGYGLQELSFTFLMSNVAGLTPAVSLTVAVLIRAF
                     FLLSSLPGAFFLPSILSAMAEQKKQSPSTTLL"
     gene            complement(2034..2984)
                     /locus_tag="VFQ23_21470"
     CDS             complement(2034..2984)
                     /locus_tag="VFQ23_21470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9909233.1"
                     /translation="MTDLSLIIPVKNEAENLPLLYKAIKQALKPIKRSWEVIFVDDGS
                     EDQSLNVLRALIEEDPNCVRVIVFRRNFGQTAAIAAGIDYAEGEIIILLDADLQNDPE
                     DIPRLLEKLDEGYDLVSGWRKDRKDNQFTRTIPSRIANGVISWVTGVYLHDYGCTLKA
                     YRRDVLGGFRLYGEMHRFIPVFAHSVGATITEIPVSHHPRKFGQANYGLERTAKVILD
                     LFTVKFLLDYSHKPIRLFGAAGAGLMLLGAADLMFLFIRRTFFGVPAFSSPLLLVGVM
                     FFIMGFQSILMGLIAELLARTYHESQGKPTYTVRTVLGKK"
     gene            complement(2981..3952)
                     /gene="gmd"
                     /locus_tag="VFQ23_21475"
     CDS             complement(2981..3952)
                     /gene="gmd"
                     /locus_tag="VFQ23_21475"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9909234.1"
                     /translation="MPTALITGITGQDGSYLAELLLSKGYRVIGVARRSSTVTYERID
                     HLLDSITVVQGDLHDQGSLLALVEEYAPTEVYNLAAQSFVPTSWNQPALTGDTTALGV
                     TRILEAIRFVNPKIRFYQASSSEMFGRVLEVPQREETPFYPRSPYGVAKVYGHWITVN
                     YRESFNMFAASGILFNHESPRRGLEFVTRKISDGVARIKLGRAKELRLGNLEAQRDWG
                     FAGDYVEAMWRMLQQDKPDSFVIGMGETHSVREFCEIAFGHVGLDYNEYVVQDEKFYR
                     PAEVDLLISDPSKARAKLGWEPAVGFKELVTMMVDADMERIRQKKIE"
     gene            complement(4102..>4983)
                     /locus_tag="VFQ23_21480"
     CDS             complement(4102..>4983)
                     /locus_tag="VFQ23_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909235.1"
                     /translation="LLISPPIALATLIIFAGWMFFTSERGGISWRTILIFGVIFIVGL
                     FFLSTSLNASGQFDASSPLSVINDWLRRAVKWNVYQIADESGMVQKIFGLREENEAPA
                     WIRLPFVAIYGLLQPVLPATILHPTKLIWMVIGFLRGLGWYALIPLLILSFGAATGSS
                     SNKVRNLILWLALFTWTWILLASIRGGGDLWDNPRYRTLLFMWQAVLAGYVWVWWRET
                     RNAWLPRILAMEGVFLLIFTQWYASRYYHIGGQLPFSVMVALIIGLWGLIIAVGWWQD
                     RGVAKRVSENRLRHREH"
BASE COUNT         1433 a         1291 c         1206 g         1053 t
ORIGIN      
        1 acacaggctg agaaagatac ttcacaatcc ctttgaaaga gtggttgttg atgactgaag
       61 caaaaggtgt ctcaagcagg tacagggcga ttgcgatcat ggccggcatg ggaaaccagt
      121 tcacccggct gatgcctgcc caaagggacg cggcgatcac tgcgatcaac gacctccagg
      181 ggtgtttcgc cgacacaaac aaaagcggga gaattaccat cacttccagg tgatagtaga
      241 cccccaccct caacaagaaa agaaaaagcc agcctgcagt cagccattga ccgtatttct
      301 cctgtgatga gaacgctcgt tttgcaattg cgagtgcgcc tgcggcggtc agcccgatcc
      361 ataaaaagaa ctgccagaag cggtgcgcga ataacccgag atcaggaatt aaaaacggaa
      421 ccgattgcaa caaataccgc gtaggatgaa gggttgaaag agggaaggat tcaccataga
      481 tccagttgga aaaatagagc gaggcatagt aatagcgact cccctccgac cagcccaggg
      541 aaagtggata agatgttacc aggttgaatc gaatcaagat ctcatacgcg acgccgagca
      601 atatcaaggc ggaggcaaac ccgatttgcc aggttgttcc aaagccaacc ttgagcgccc
      661 acatgcccag taacaatgcc caccagcaca gccagtaata cagatacagc accaggatgg
      721 ggtcacgcgt cgtttctttg acggcctgac caagctcgaa tctgaattta atatagggaa
      781 ttaagatcaa tattcccaaa aataatagga tccccaaaac gcgccataac aggttatcaa
      841 acttcacatg gcttaatcgt gatatcaggc tgaacggcaa cgtgtcggag cgaacgatag
      901 agaagatcag ccaaagacag atcaacatga ccgccacgca tatgaccagc attccgctcc
      961 aggtggcgcg cggtaaatta gcgccgatct cgatcagacg cagataggtc aggcgcagca
     1021 tcgtcagatt gaccatgaag gtgaagatga gaatggtcaa ccagaaagag cgggaagcgt
     1081 tttgcgcctg attcatggaa gatcaaagga gggtagtgga aggcgactgc tttttttgtt
     1141 ctgccatggc agagagaatg gatggcaaaa aaaatgcacc aggcaggctg gatagcagga
     1201 aaaaagcacg aatgagcacg gcaaccgtga ggctaacggc cggggtcagc ccagccacat
     1261 tggacatcaa aaaggtaaat gaaagttcct gtaagccata cccgttgatc gagatcggta
     1321 tgagggtaat gaaataggtg aggctccaca agcccgcgat catccagaaa gaaatgtgac
     1381 tgccaagatc gttcacgaac acataaattg atccaaaaag gcaaagcatg tggatccagg
     1441 taaacgccag cgaccccagc aggaaggcag gtttttttaa ccaaattgaa aatatggaag
     1501 cggtccgttt aataaatgcg accggttttt gaaggacacc caccaatgaa gacgaggcaa
     1561 ctccaagctg gatcatgttc catgaatacg acaatccaat tggaaccgtc atacccatgc
     1621 caagcattcc aatcaaacga tcggccgcga tggatgcaag acaaaccgca cgatcaaagc
     1681 ccatctgcat tgcacccgcc agtcggacca catcccctcc gatggtggtc ggcaggaagt
     1741 tcgatgcaaa cagtccggta aacgtcaacg caaggctttt ctttagcggg atatgaacgc
     1801 cacccgattg caacagcatg tgccagcgcc aggccactgc cagacgtgag atggcaaaca
     1861 agattgtgac taaaattaaa tcgaggatgg taatcttttg catggcagct acgatttccc
     1921 cccattcatc attccgtacc aacaaaatga tcataacgac cgctaacaat gttcccacca
     1981 attgcagcag gcgctgacgg ttccgtaacc accaggaccc tttgggcata atcttacttc
     2041 ttccccaaca cggtccgaac ggtataggtt ggctttccct gagattcgtg ataggtacgc
     2101 gccagcagtt cagcaatgag ccccataaga atggattgaa aacccataat gaaaaacatc
     2161 acgccgacca acaaaagagg agaactgaag gcaggcacac caaagaacgt acgacggatg
     2221 aataaaaaca tcaaatccgc cgcgcccaac aacatcagtc cagccccggc agcaccaaac
     2281 agacgaatcg gtttgtggga ataatccaga agaaatttta cagtaaaaag atcaaggatg
     2341 accttcgcgg ttctttctag cccgtaattt gcctgtccga atttacgagg gtgatggctg
     2401 acgggaattt ctgtaatcgt tgcgccgaca gaatgggcga acaccgggat gaaacgatgc
     2461 atttccccat acaagcgaaa cccgcccagc acatcgcggc gataggcttt gagcgtacag
     2521 ccatagtcat gaagatatac ccctgtcacc catgagatca ccccattggc aattcttgac
     2581 gggatcgttc gggtaaattg gttatctttt cgatccttgc gccagccact gaccaggtca
     2641 tagccttcgt cgagtttttc cagcagtcgc gggatgtctt cgggatcatt ctgcaaatca
     2701 gcgtcgagca ggatgatgat ctcgccttct gcgtagtcaa tcccggccgc gatcgcggcc
     2761 gtctgaccaa agttgcgacg aaagacaatc acacgaacac aattcgggtc ttcctctatc
     2821 agcgctctta atacgttgag actttgatcc tcacttccat catcgacaaa gatcacctcc
     2881 caacttcgct tgatgggttt cagtgcctgt ttaattgctt tatagagaag aggcagattc
     2941 tcagcctcgt ttttgacagg aataattagg gagagatcag tcattctatc tttttctggc
     3001 gaatacgttc catatccgca tccaccatca ttgtgacaag ttccttgaaa cccacagcgg
     3061 gttcccagcc aagtttcgca cgggctttgg aaggatcaga gattaataga tccacctcgg
     3121 ccggacggta gaatttttcg tcctgcacaa catattcatt gtagtccagg ccgacatgac
     3181 caaaggcaat ctcacagaac tcgcgcacgg aatgtgtttc gcccatgcca atgacaaagc
     3241 tatcgggctt atcctgctgt aacattcgcc acatcgcttc tacataatca cctgcaaagc
     3301 cccaatcacg ttgtgcttcg aggtttccca gccgcaattc tttggcacgt ccaagcttga
     3361 tgcgcgccac accgtccgag atcttgcgtg tcacaaattc cagcccgcgg cgcgggcttt
     3421 catgattgaa caaaataccg gaggcggcaa acatattgaa ggattcgcga taattaactg
     3481 taatccaatg tccgtatact tttgcaacac cgtatggact gcgcggataa aagggcgtct
     3541 cttcccgttg tggaacttcg agtacccggc cgaacatttc gcttgacgaa gcctgataaa
     3601 agcgaatttt ggggttaacg aaacggatgg cttcgagtat tcgagtaaca cccaaggccg
     3661 tagtgtcgcc tgttagtgct ggttgattcc aggaggtagg aacaaacgat tgagctgcca
     3721 ggttatagac ttctgtgggt gcatactctt ccaccagggc caggagcgag ccctggtcat
     3781 gcaggtcgcc ctgaacaact gtgatactgt ccaacaggtg gtcaatccgt tcataggtca
     3841 ccgtactgga acgacgcgcg acgccaatca cacgatatcc ttttgaaagc agcaattctg
     3901 ccaggtatga tccgtcttgt cccgtaattc cggtgattag tgcagtgggc atccgttctc
     3961 cttaggtcaa aattcaaccg ctgaattata acccacattc ataacctgcc agttttcttt
     4021 cgatcctggg gagtggcgaa tttagtgtgt aagccacaca cctgcctgat ttgcttaaca
     4081 aatcatgcgg cgtcaaaggg ctcagtgctc tctgtggcga agtcgatttt cactcactct
     4141 cttagccact ccacgatcct gccaccagcc tacagcaata atcagccccc ataagcctat
     4201 aatcagggcg accatgacag aaaatggcag ctgcccaccg atgtgataat accgactggc
     4261 ataccattgg gtgaagatca atagaaagac cccttccatc gccaaaatcc tcggcaacca
     4321 cgcgttccgt gtttctctcc accagaccca cacatacccg gcaaggacag cttgccacat
     4381 aaataggagc gtccggtagc gcgggttgtc ccacaggtcg ccgccgccgc gtatggaagc
     4441 aagcagaatc caggtccatg tgaagagcgc caaccagagg ataagatttc gcaccttatt
     4501 ggaactcgat ccggtcgcgg ctccgaagga gaggatcaat agcggaatca gcgcatacca
     4561 acccagcccg cgcagaaacc cgatgaccat ccagatcagt tttgttgggt gaaggatcgt
     4621 cgcaggcagg actggctgaa gaagaccata aatcgcgaca aacggcaggc gaatccatgc
     4681 gggagcttca ttctcttcgc gtaaaccaaa tatcttttgc accatgccgg attcatctgc
     4741 aatttggtac acattccatt tcacagcacg ccgaagccaa tcgttgataa cacttaatgg
     4801 agaggaagca tcaaattgac ctgaggcgtt gagagaggtt gaaagaaaaa ataaccctac
     4861 tataaaaatg acaccaaaga tgagaatcgt tctccatgag ataccgccgc gttcactggt
     4921 gaagaacatc cagccggcga agatgataag agtagcaagc gcgatgggag gggagatcag
     4981 cag
//
LOCUS       DASTJP010000738         8199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1026932, whole genome shotgun sequence.
ACCESSION   DASTJP010000738 DASTJP010000000
VERSION     DASTJP010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026932"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFQ23_21485"
     CDS             complement(<1..412)
                     /locus_tag="VFQ23_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET9909236.1"
                     /translation="MAKITMREAISQALMEEMDRDPAVFIIGEEVGVWGGTYAVTKGF
                     YDKYGPDRIKDTPIAENAIIGAAIGAAMVGQRPIAELMTINFAFSAMDYIVNQAPKLH
                     YMFGGQFKVPMVIRAVGGGGRQLGATHSQTPDAIF"
     gene            complement(642..1601)
                     /gene="pdhA"
                     /locus_tag="VFQ23_21490"
     CDS             complement(642..1601)
                     /gene="pdhA"
                     /locus_tag="VFQ23_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET9909237.1"
                     /translation="MKKEQQLDLYHQMVLIRRVEERGAELYQAGKIGGFMHLYIGQEA
                     VSTGLIAAREPRDRVITAYRDHGVAINCGIPATEVMAELLGKATGMSKGKGGSMHMAS
                     VEKNFWGGHAIVGGHLPIAAGLATGDQYAKNDNVTICMFGDGATNIGYFHEALNLAKT
                     WGLRVLWVCENNVYGMGTSVERASAVKEIRQKAEGYSMKNSRVDGMDVMKVYEAAKEA
                     IGFVRENSQPYFLEVDTYRFRGHSMGDPERYRKQEEVKKWQENDPIGIFRKHLIEKKT
                     ATAKALDEIDAKVEVEVNEAVEFAETSPEPAMEELFTDIFVEN"
     gene            1865..2038
                     /locus_tag="VFQ23_21495"
     CDS             1865..2038
                     /locus_tag="VFQ23_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909238.1"
                     /translation="MRLSPPKVITFWISVALGLLGLLGSFGVLGTALAGFSFWLVFLG
                     FALLAVSLMVDGL"
     gene            2106..3245
                     /locus_tag="VFQ23_21500"
     CDS             2106..3245
                     /locus_tag="VFQ23_21500"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9909239.1"
                     /translation="MNWLTGGKQGEAKRLIAQLSDPTRREHAAQELIRLGSDAVPALV
                     ESLATQDLNLLSLYQQILARIPSASPSLLKVLGTAHPVIRGRAAEVISLSKDRTAVPA
                     LLEALQGEYFTVRSRAALALGKIGDRKALPPLLHALKDPEDEVRIAACLALGFFKDPS
                     TFDEITNVLLDDPQIEVRQSAAKALGNTEHPAALPFLMEALHDSYWWYEREYAAVDLL
                     QAIEKMGKTAIDPLIDALQDKEGTVRKFAAALLGKLGEPRAIEPLGMALYDLHHDVGS
                     AAAESLARFGASAVEVLIEALSHPEMWIRIHAVSALGKIRDARVTPVLLEMLNDPERE
                     VRKHVIKILGELKDTRALSALQEIASNRADRELSMLAKQSLNVSN"
     gene            3280..4500
                     /locus_tag="VFQ23_21505"
     CDS             3280..4500
                     /locus_tag="VFQ23_21505"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HET9909240.1"
                     /translation="MTAEGAAPIAPSKPKDRILDIDILRGFALFGVILVNIFYFHVPA
                     PYFSAYYEQFHDPLNRGVFYILNWFFTGKFYPIFSFLFGLGFSIQFLSAKQKGVNPYA
                     FLVRRLTILLFFGLAHIIFVWEEDILFIYAVFGFVLLALIEQSPTFILSAALLLYFAS
                     PAFDIIDHFFKLSSNVPALFDSVQSYADFYVNASYWQILQERLAIYVQKYDQPGELIS
                     LLNRLAFFLIGLYAGKMNYIATFRENFRVWYKFLILSLFIFSIGFVVDKIWLQDLDKA
                     RNSALLLSVEGVENSVSGLFQVFVYIIGFLFLLTFSRIRPFLLPLTNIGRTALTVYLT
                     HTIVFSFLFYSFGLRLYASLSPIQLFVIAIVLFFVDVVASILWLRYYQYGPLEWVWRS
                     LTYKKALPLRKQAI"
     gene            4574..5272
                     /locus_tag="VFQ23_21510"
     CDS             4574..5272
                     /locus_tag="VFQ23_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909241.1"
                     /translation="MMKRIHLLLAVQSVVAILVSINRLGTWTLGYVLPNEFLRWVDFN
                     NMLVLPLISLVALYLLKKTIEYDSPARDGRAHLILNLTFIIGLYFLGASYGDHEVTNY
                     LHVRFCLDDPSSDLCRINIFNDDEFSHWVFFAGFFMVNAALLFLQEIFPYRGQLSSSD
                     IGFLSFNALFLGAGILANLGFEEIGLDLYIVALLTFLSTYLLWKRGRQPLFIYYTAAF
                     WLGLIASFIAQVMR"
     gene            5292..5414
                     /locus_tag="VFQ23_21515"
     CDS             5292..5414
                     /locus_tag="VFQ23_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909242.1"
                     /translation="MTTASILKNATDEQLALAVFENLYHLFRAMAANLPCLTGS"
     gene            5396..6178
                     /locus_tag="VFQ23_21520"
     CDS             5396..6178
                     /locus_tag="VFQ23_21520"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909243.1"
                     /translation="MPDGQLIESEKLSRHLTFPTNPMFKGVWNTRLSESDVDEVIRET
                     IIWFKEQNAPYLFWWTGGNTSPADLEERLAEQGLISMAEQTQELAKGILSTEQGSPCM
                     IAELSHMNEDVLEQTPRDFAIREISNEPELYDFKNVFVETYEIPEWAGQAWVDATLQI
                     GIGKTPWRMFVGYFDNKPVATNMLFNGGGVASVYAVATIPSARGKGLGGAITLKPLLE
                     AREKDGYQYAVLFSTEMGIKVYERIGFRKTNVRINRYLWRNG"
     gene            6260..6769
                     /locus_tag="VFQ23_21525"
     CDS             6260..6769
                     /locus_tag="VFQ23_21525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909244.1"
                     /translation="MTYFIWLLRIVHIVAGIFWVGGALIMAFFISPTIGATGEAGQKF
                     VGHLMNNLKFSTRMMIASGLTILAGFILYWIDSDGFTSAWMQSGAGTGFGIGAAFALI
                     GLVFGILVGRTTNAMAKLGAQFQGKPSGEQMSQLGALRTQQIRYSYLNAAMLVISVIF
                     MAIARYLVF"
     gene            6914..>8199
                     /locus_tag="VFQ23_21530"
     CDS             6914..>8199
                     /locus_tag="VFQ23_21530"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9909245.1"
                     /translation="MVETTENTAVRKNASVMISYSRKDLAFVRKLFESLIAHGFAKEE
                     IWVDWEGIPLTADWMAEITEGIQSSDAFIFVISPDSLSSEVCAKEIAIAVEANKRFIP
                     ILHRDPPKGTALPAKISSHNWVFMRDEPELEKNLPPLVAALNTDLDWLAQHTRLLNRA
                     LEWERKGHNDSYLVRGSDLQEAQSFIELGATGKEPPPTTLQVEYVKSAREHAAVLRRR
                     NRIIAAVVGVALLVLSVFSLIQWGNALVQRDRADANANTAVANEQIAKAQEELAIEAQ
                     ARAEMEARVANAQALAAEAFNQKDNDSQLALMLSLLSIQETESDGIVLPESKSALFAS
                     LNSPNVLYTFPETNGVVWAVAIDPNGMYAATCDELGVSIWNLRTRELIRLIEVGFITN
                     GLDFSPDGTRLGIAGDIGKVVDVQSGQTVFELQGHEG"
BASE COUNT         1934 a         1969 c         2025 g         2271 t
ORIGIN      
        1 caaagattgc atcgggtgtt tgtgaatggg ttgcgccaag ctgacgtcca ccaccaccaa
       61 cagctcggat caccatcggt actttgaact gtccgccaaa catatagtgc agctttggtg
      121 cctggttgac aatataatcc attgccgaaa aagcaaagtt gatggtcatc agttctgcga
      181 ttgggcgctg acccaccatg gctgcgccga ttgccgcgcc gatgattgca ttttctgcga
      241 tcggtgtatc tttgatccga tcaggaccgt acttgtcata aaaacctttg gtcaccgcat
      301 aggttccacc ccaaacgccc acttcttccc cgataataaa tactgccgga tcacgatcca
      361 tttcttccat cagtgcttgc gagatcgctt ctcgcattgt gatttttgcc atgtatatcc
      421 tcttaagtat gtctttgcga agatggtcgg agacccgacg acacttgcac ccgatttttg
      481 aaatgcttgt gcatttcatg gtaaatcgtg cttccggtgc agtgcaggtg agcaatctca
      541 acgaaccaat aatttcgaga ttgctacgcc tgcttcgcag gctcgcaaca cttgcatgat
      601 ttgccaagca aatcacaccc aaccaagtgc aggtgtgact tttaattttc cacaaaaatg
      661 tctgtgaaca gctcttccat cgcgggctcg ggactggttt ccgcaaattc tacagcttca
      721 ttgacttcca cttcaacttt tgcgtcgatt tcgtccagcg ccttggctgt tgcagtcttc
      781 ttttcaatca gatgtttacg aaagatccca atcggatcgt tttcctgcca ctttttcact
      841 tcttcctgtt tacgataacg ctcagggtca cccatggagt gacctcggaa acgatatgta
      901 tccacctcaa gaaaatacgg ctgagaattc tcacggacga aaccaatcgc ttccttggct
      961 gcttcataca ctttcatcac atccatgccg tccacgcggc tgttcttcat cgaatagcct
     1021 tctgcttttt gacggatctc tttgaccgcc gaggcccgtt cgacagaggt gcccatccca
     1081 tagacgttgt tttcgcaaac ccacaagacg cgcaagcccc acgtttttgc cagattcagc
     1141 gcttcatgga aatagccgat gtttgtcgcc ccgtcgccga acatacagat ggtcacatta
     1201 tcatttttcg cgtattgatc gccggtggca agcccggctg cgatcggtag atgcccacca
     1261 acgattgcat gcccgcccca gaagtttttc tccacacttg ccatgtgcat ggagccacct
     1321 tttcctttgg acataccagt ggctttgccc agcaattcag ccatgacttc agtcgctgga
     1381 atgccgcaat tgatagcgac accatgatcg cgatatgcgg tgatcacccg gtcgcgtggt
     1441 tcccgcgcgg cgatcaaacc tgtcgagacg gcttcctgcc cgatatacaa atgcatgaag
     1501 ccgccaatct tgccagcctg gtacagttcg gcgccacgtt cttccacgcg acggatgagc
     1561 accatctggt gatacaaatc aagctgttgt tctttcttca tcgtaactcc caataaaaat
     1621 gattgcaaag ttcaggcaaa ataacaccgg ggcagccccg gcgatgtgct ttcctctctc
     1681 cacataaaaa ttataacaga gattacaggc tcaattcctg actgcgatga cccatagatt
     1741 tataaatttt aagagaatca gcggatataa gtattgaatt atgaataaga atttgctata
     1801 acgaatatag tgattcgcca gttcgttttt gaatcagtac gttaatggag aaaaggagaa
     1861 tgcaatgaga cttagtcccc ccaaggtaat tacattttgg atttctgtag cgctcggctt
     1921 gcttggtttg ctcggcagtt tcggtgtgct tggcacggcg cttgcagggt tttcgttctg
     1981 gcttgtattt ttgggattcg ctctgcttgc agtgagtctt atggtggacg gcctataaga
     2041 ttggcttttg gcgaatcacg actcctcgcg tcgcgaggag ttttctttta agataaaata
     2101 aaactatgaa ctggctcact ggtggaaagc aaggcgaagc caaacgcctc attgcccagc
     2161 tttcagaccc aacaaggcgc gaacatgctg cacaggaatt aattcggctt ggctcggatg
     2221 ccgtacctgc actcgttgag tcgcttgcca ctcaggactt gaatctgctc tcgctttatc
     2281 aacagatcct cgcccgaatt ccatccgctt ccccctcatt gctcaaagta ctcggcactg
     2341 cgcatcccgt cattcgcggg cgcgcagcag aggtaatttc actcagcaag gatcgaacgg
     2401 ccgtgccggc tttattggag gcacttcagg gtgaatactt tacagttcgc tctcgagccg
     2461 cgctggcgct ggggaaaatc ggtgatagaa aagcacttcc tccgttgttg catgccttga
     2521 aagacccgga agacgaagtt cgcatcgcgg cgtgcctggc gctgggtttt ttcaaggatc
     2581 cttccacctt tgatgaaatt acaaatgtct tgcttgatga tccacagatc gaagtacgtc
     2641 agtccgctgc gaaagcgttg ggaaacacag agcaccccgc ggcattaccg tttctcatgg
     2701 aagcgctgca tgattcgtat tggtggtacg aacgtgagta tgctgcggta gatttgcttc
     2761 aggcgatcga aaagatgggc aagactgcga ttgatccgct tattgatgcc ttgcaggata
     2821 aagaagggac ggtgcgaaag tttgctgcag cactgctcgg taagcttgga gagccgcgcg
     2881 ccattgagcc gctgggtatg gcgctctatg atctgcatca tgatgttggc agcgccgctg
     2941 cggaatcgct cgcccgattt ggtgcatccg cggtcgaagt gctgatcgaa gctttaagtc
     3001 atcctgaaat gtggattcga atccacgctg ttagcgcgtt aggaaaaatc agagatgcgc
     3061 gggtaacgcc tgtacttttg gaaatgctga acgacccgga gcgtgaggtg aggaaacacg
     3121 ttatcaaaat tcttggggaa ttgaaggata ctcgcgcgct atccgcattg caggaaatcg
     3181 catccaaccg cgccgatcgg gaactctcga tgctggcaaa acaatcactc aacgtctcga
     3241 attgaaaatt tgcgggataa acaattttgt tgattatcca tgacagccga aggcgccgcc
     3301 ccaatcgcac ccagtaaacc gaaagacaga attcttgata tcgatatcct gcgtggtttt
     3361 gcgttgttcg gtgtcattct cgtaaacatt ttttactttc acgtcccggc tccttatttc
     3421 agtgcctatt acgaacaatt tcatgatcct ttgaacaggg gtgtttttta tatcctcaac
     3481 tggtttttta ccgggaagtt ctatccgatc ttttccttcc tgtttgggct agggttttcg
     3541 atccaattcc taagtgcaaa gcaaaaaggt gtaaatccct atgcgtttct tgtacgccgg
     3601 ctgacgatcc ttttgttttt tggactggca cacatcattt ttgtgtggga ggaggatatt
     3661 ctttttattt atgccgtttt tggtttcgtt ttgctggccc tgattgaaca gtctcccacg
     3721 ttcattctca gcgcggcact gcttttatat ttcgcttcgc ccgcattcga catcatcgat
     3781 cactttttta agcttagttc gaacgttcca gcccttttcg attccgtcca atcgtacgct
     3841 gatttttatg ttaacgcttc ctactggcaa atccttcagg aacgcctcgc catttatgtt
     3901 caaaaatatg atcaacccgg agagctcata tcattactga accgtctggc cttctttctc
     3961 atagggctgt acgctggcaa gatgaattac attgccacct tcagagagaa tttccgcgtt
     4021 tggtataaat tcttgatatt gtccctcttt attttctcca tcggatttgt agtggacaaa
     4081 atatggctgc aggatttgga caaggcgcgg aactctgcgt tgcttctctc tgtggaaggt
     4141 gtcgaaaatt cggtttcagg cctctttcag gtttttgttt atattatagg ctttctgttt
     4201 cttctgacct tcagccgcat aagaccgttc ctgctccctc tgaccaacat cggcaggacg
     4261 gcactgaccg tttatttgac tcacaccatt gtcttctctt tcctgtttta ttcttttggc
     4321 ttaagactgt atgcatcatt gagtcctata cagctcttcg tgatcgctat cgttcttttt
     4381 tttgtggatg tggttgccag cattctctgg ctgagatatt accaatatgg accgctggag
     4441 tgggtatgga gatcgcttac ctacaagaaa gccctgccgc tgcggaaaca agcgatctaa
     4501 caaaaatccc agatgcatta ggaggagaga tcctatttat gcatttcgac tttgtagctg
     4561 gctttgagta gaaatgatga aacgtattca tcttttgctg gcggtgcaat ccgttgttgc
     4621 gattctggtg tccatcaacc gcctgggaac ctggacactt ggctatgttc tgccaaatga
     4681 atttctgcgc tgggtggatt tcaacaatat gctggtcctg ccgctcatca gcctggttgc
     4741 gttatatcta ctgaagaaaa caattgagta cgactcaccc gctcgcgatg gacgcgctca
     4801 cctgatcctc aatctgacgt ttatcatcgg gctgtatttt ctcggcgcaa gctatggcga
     4861 tcatgaagtg acgaattatc tgcacgtccg tttttgtctg gatgatcctt cgagtgattt
     4921 gtgccgcatc aatatcttca atgatgatga attcagtcat tgggtattct ttgccggttt
     4981 tttcatggtc aatgcggctc tgctgttcct gcaagaaatc tttccatatc gaggccagct
     5041 cagcagcagc gatatcggtt tcctgagttt caatgccttg ttcttaggtg cggggatcct
     5101 ggcgaatctg ggctttgaag agatcgggct ggatttgtac attgtggcgc tgcttacttt
     5161 tctttctaca taccttttat ggaagcgtgg caggcagccg ttgtttattt attacactgc
     5221 agcgttttgg ctgggcctga tcgcaagttt tatcgcgcaa gtaatgagat gacattgcaa
     5281 gggaaaatat catgacaaca gcttccatcc tcaagaatgc gaccgatgag caactggctc
     5341 tggctgtctt cgaaaacctc tatcacttat tccgcgccat ggcagcaaac ttgccttgcc
     5401 tgacgggcag ttgattgaaa gtgaaaagtt gtcgcggcat ctcacctttc ccaccaaccc
     5461 gatgttcaaa ggggtttgga atactcgtct ctcagagagt gatgtggacg aagtgattcg
     5521 cgaaaccatt atctggttta aggaacagaa cgctccctac ttgttctggt ggaccggagg
     5581 aaacacatcc cctgcagatt tggaggaacg gcttgcagag cagggtctga tttccatggc
     5641 cgagcaaacc caggaactcg ccaaggggat cctctccacc gagcagggat caccctgcat
     5701 gattgccgag ttgagtcaca tgaacgaaga tgtccttgaa caaactccca gagactttgc
     5761 tatccgagaa atatctaacg agcccgagtt atatgacttt aagaatgtat ttgtagaaac
     5821 ctatgagata ccagaatggg cagggcaggc ctgggtggat gccacactac agattggcat
     5881 tggcaagacg ccctggagaa tgttcgtcgg atattttgat aataaaccgg tcgccacaaa
     5941 tatgctcttc aatggcgggg gagttgcaag tgtatacgcc gtcgcgacga ttccttcggc
     6001 gcgcggcaag gggcttggtg gagcgattac actcaagccg ctgctggaag cgcgtgaaaa
     6061 agatggatat caatacgcag ttctcttttc gaccgaaatg ggtatcaaag tctatgagcg
     6121 gatcggtttt cgcaagacaa acgtccgcat caatcgctat ttgtggagaa atggataact
     6181 cctggggcat acaccgtatc aattttcctt tatgtgggtt ttgttcttag gctgaatggt
     6241 tcgagaaaag gagaatagaa tgacgtactt tatctggtta cttcgcattg ttcacatcgt
     6301 agcgggtatc ttttgggttg gcggtgccct gatcatggcc ttcttcatta gcccgacgat
     6361 cggcgcgacg ggagaggcag gtcagaaatt tgtgggacac ttaatgaata atcttaagtt
     6421 tagcacccgt atgatgattg cctccgggtt gacgatcctt gcagggttca tcttatactg
     6481 gatcgactcg gatggcttta cctctgcctg gatgcagtcc ggggcgggaa ccggttttgg
     6541 aatcggcgca gcctttgcgt tgattgggtt agtgtttggc attctggttg gtcgtacaac
     6601 gaatgcgatg gccaaacttg gcgcacaatt ccagggaaaa ccatctggcg aacagatgtc
     6661 acaattgggt gctttgcgca cgcaacagat caggtattca tacctgaatg cggcaatgct
     6721 ggttatctcc gtaatattca tggcaattgc gcgttatctt gtattttagg tcagcgtggc
     6781 tgagacaatg tagactagac tctcagaagt aaaaattact tcctgtggac gaaataatat
     6841 agtgcctgtg aaacaatcag gtgcgtgata ttaattctaa atgggcagga tatcaaaagg
     6901 aaacatcgaa gcaatggtgg agacaacaga aaacacagca gttcgtaaaa atgccagcgt
     6961 aatgatttcc tattcacgca aagacctggc tttcgttcgt aagctctttg aaagtttgat
     7021 tgcacacggt tttgccaagg aagaaatctg ggtggactgg gaaggtattc ctctgacggc
     7081 cgattggatg gcggagatca cggaaggtat acaatcttct gatgccttta tctttgtcat
     7141 tagccctgat tctctttcct ccgaagtctg tgcgaaggaa attgcaatcg ctgttgaagc
     7201 caataagcgc ttcatcccaa tcctgcatcg tgatccgcct aaaggtacag ccttgcccgc
     7261 caagatctct tctcataact gggttttcat gcgcgatgag ccggaattgg agaaaaacct
     7321 tccgccgctg gtggcagcgc tcaacactga cctggattgg cttgcgcaac atacccgttt
     7381 gctaaaccgt gcattggaat gggagagaaa gggacacaat gatagttatc tcgtacgcgg
     7441 aagtgatctg caggaggcac aaagcttcat agaactcggg gccacaggga aggagccgcc
     7501 tccaacaacc ttgcaagtgg agtatgtaaa gtctgcgcgc gagcacgctg ctgtgctccg
     7561 ccggcgaaat cgaatcattg cggcagtagt tggcgttgcc ttattggtac tgagtgtttt
     7621 ttcgctgatc caatggggga atgctttagt acaaagagat agagctgatg cgaatgccaa
     7681 tactgcggtt gctaacgaac agatcgcaaa agcgcaggag gagcttgcca tcgaggcaca
     7741 ggcccgggcc gaaatggaag cacgggtggc gaacgctcaa gcgctggcgg cggaggcgtt
     7801 caatcaaaaa gacaatgaca gtcaactcgc tttgatgttg tcattattgt ccatccagga
     7861 aacggagtcg gacggtattg tgctgcctga atccaaatcg gccctgtttg caagtttgaa
     7921 ttcccccaac gttttgtata ccttcccgga gaccaatggc gtggtatggg cggttgccat
     7981 tgacccaaat ggtatgtatg cggccacctg cgacgaactg ggagtgtcaa tctggaatct
     8041 gcggacccga gaactgatcc gtctgatcga agtcggtttt attaccaacg gattggattt
     8101 cagcccggat ggcacacgct tggggatcgc aggtgacatc gggaaagtcg tggatgttca
     8161 aagtggtcaa acggttttcg aactgcaggg tcacgaggg
//
LOCUS       DASTJP010000739         5231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1052173, whole genome shotgun sequence.
ACCESSION   DASTJP010000739 DASTJP010000000
VERSION     DASTJP010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052173"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..267)
                     /locus_tag="VFQ23_21535"
     tRNA            complement(194..267)
                     /locus_tag="VFQ23_21535"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Pro,seq:ggg)
     gene            448..1044
                     /locus_tag="VFQ23_21540"
     CDS             448..1044
                     /locus_tag="VFQ23_21540"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET9909246.1"
                     /translation="MPDMIEVQIDSVRVHLMTPSRLVVLKQINSERYLPIWVGPYEAE
                     AITVALQEVEMVRPLTHDLLKNVFGAFNARITRIEIVKLENDIYYGSIVAEVDGREIF
                     VDSRSSDAIALSVRAHVPILVHPSVMDSAGIVPEQDMPENGDQPEKSEPAPLTKEGAE
                     RLTVFEDFLGKLDLDKLDDKDQTDDDEPPEKPKKPKKK"
     gene            1041..2426
                     /gene="dnaB"
                     /locus_tag="VFQ23_21545"
     CDS             1041..2426
                     /gene="dnaB"
                     /locus_tag="VFQ23_21545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET9909247.1"
                     /translation="MTNPLPPEIPFGDTAPTTPTAVPHSREAEEAVVGAVFINPEVYY
                     DVAQFLNADDFYIHRHRWVWEAFTSLHEQRIPIDLLTVSDELDRRGQLSEIGGPAYLT
                     ALVNQVPSSLNAESYGRIVEGYAVRRKMITAANQIASIAYNERTSVDDVMNEAEKAVF
                     NVSERRLKHDLQPISSVLSEYYDRIDDLAKRPDEFFGVPTGFIDLDKMLSGLQPSDLL
                     IIAGRPGQGKTGFLLSIAKNASLTHKKHVAIFSLEMSNEQVVQRLIAQETGIDSQRLR
                     TGKLEETEWPLFTHAIEVFSDTHVYLDDTPAITPLQLRTKCRRLHMEFGLDLVIIDYL
                     QLMGGDTRNDNRVQEVSYISRNLKVLARELNVPVLAAAQLSRAVEQRSDKRPVLSDLR
                     ESGSLEQDADIVMFIYRPDQYEKDTVKQNVAEIIVAKHRNGPVGSVELVFRNALAKFE
                     NAATRIFKPNE"
     gene            2471..3136
                     /locus_tag="VFQ23_21550"
     CDS             2471..3136
                     /locus_tag="VFQ23_21550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET9909248.1"
                     /translation="MKTFKGFTDSETFTQLPNAFFHQLIHQIDDIAELKVTLFVMWHV
                     DHMDGPFRALSKMDFDVKELGLGADEITRGLEKAVRRGSLLTVNAPRDAMVYFLLNSP
                     RGRSAIQAIEKGKWNPADGVSAPILERPNIFKLYEENIGPLTPLIADALKDAEQTYAD
                     EWIAEAIELAVKNNKRYWKYCEAILKRWKEEGRHGKKDQQDSVQSSERYTKSEFAEYL
                     ESE"
     gene            3051..4577
                     /locus_tag="VFQ23_21555"
     CDS             3051..4577
                     /locus_tag="VFQ23_21555"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909249.1"
                     /translation="MEKKISKTLSRVLNDTQKANSQNISNLSNPAMQMFGKPDCPHCG
                     GVGYLRTDVPVGHEKFGKLEPCVCRASDIAANARQRLYELSNLERLSHLTFENFNRSG
                     NPKAEFISPQEAASLHEAYTSSETFARDLQGWLLLEGSYGGGKTHLAAAIANAAVQRG
                     VPTLFITVPDLLDSLRFSFSDATTSFESRFDDIRDAALLVMDDFGTHNATPWAQEKLF
                     QIINYRYINKLPTVITTNLILDEVESRIRSRLQDADYVKHVRITSADYRRPKETSNPG
                     ISMLSFTDIKEMSFGNFETRDDEVGKEIVTTIVKERQDRYGRPFKDKEIVREKVTQQH
                     IKRLHDALDAAVQFAENPNGWLVFLGGSHSGKTHLAAAIGNYRLLLGGQAILVEVSNL
                     LDYLKSTFRPNSDVTFDRRSYEIRTTPLLMLDDLKEGGISSVWAEDKLHQILNHRYYA
                     NLPTVITSTLDPESFAKSYPSLWNKILDPAKCRVCVIDMPPYRRAAKVKKPAVSKKVK
                     "
     gene            complement(4641..>5231)
                     /locus_tag="VFQ23_21560"
     CDS             complement(4641..>5231)
                     /locus_tag="VFQ23_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909250.1"
                     /translation="IHWVDRGGDVTYHGPGQLVGYPLLPLRPVRSSEDAELQGDSSAH
                     TAEATLDTLRPDRSDSRIPQAHYVGYVRNLEKTLITALARLGLPAGQRPGLTGVWIQA
                     DVHSRCPRCKPEDRKKPAKIAAIGVKVDARGISRHGFALNVNPDMEYWEGIIACGLQD
                     EPIVSLADLFPEPPSMERVKQEVITAFSEVFDFQTK"
BASE COUNT         1401 a         1288 c         1315 g         1227 t
ORIGIN      
        1 agggaggtcg gatttgctgc tcgcgtcccc gtaggggaaa cctacgaccc actaaaagca
       61 aaagactcca tttctcgagg tctcctcgag tcctccgtct gtcggggcga taagatttgc
      121 tgctcgcgtc cccgtagggg aaacctacga cccactaaaa gcaaaagact ccatttctgg
      181 agtcctccgt ctgtcggggc gataggattt gaacctacga ccccttgcac cccatgcaag
      241 tgcgctaccg ggctgcgcca cgccccgatt gataggtgct gattatagtc tcatttgaat
      301 aatctggcaa ctcgatgaca ttaacctttg attatgctaa aatacatgga aaggtgttat
      361 tatcgaaaca catttcattg caagccaagg cgatcaccaa tttgaaaaac aatgaaagat
      421 atgtgcaagt aatctgctgg agttcccatg cccgacatga ttgaagtgca aatcgacagt
      481 gtccgtgtcc acctgatgac accaagccgt ttggttgtat taaaacaaat caactctgaa
      541 cgatacctgc ctatttgggt ggggccctat gaagcggaag ccatcacggt cgctttacag
      601 gaagtggaaa tggtacgccc gctgacacat gacctgctca aaaatgtctt cggcgcgttc
      661 aatgctcgaa ttacacggat cgaaattgtc aaactcgaaa atgatatcta ctatggctcg
      721 attgtcgctg aagtagacgg acgagagatc tttgtcgact ctcgatcctc agatgcgatt
      781 gcattatcgg tgcgcgcgca tgtaccgatc cttgttcacc caagcgtgat ggattcggcg
      841 ggaatcgtcc ctgagcagga tatgcctgaa aacggtgacc aacccgagaa aagtgaacct
      901 gctccattga ccaaagaagg ggcggagcgt ctcaccgtat ttgaagattt cctcggaaag
      961 ctggacctgg ataagctgga tgacaaagat caaactgacg atgacgaacc acctgagaaa
     1021 ccgaaaaaac caaagaaaaa atgaccaatc ccctgccccc tgaaatcccc tttggggata
     1081 ccgcccccac aactccaacg gcagtgcctc acagccgaga agccgaagaa gcagtcgttg
     1141 gggcggtatt cattaatccc gaagtctatt acgacgtcgc tcagtttttg aatgcggacg
     1201 atttttacat tcacagacat cgctgggtgt gggaagcatt taccagcctc catgaacagc
     1261 gcattcccat tgacctgttg acagtctccg acgaacttga ccgtcgcggc cagctaagcg
     1321 aaatcggcgg gccggcctat ttgactgcgc tcgtgaatca agtgccgtcc tccctcaacg
     1381 ccgaatcgta tggccgcatc gtggagggat atgccgttcg ccgcaagatg atcactgcgg
     1441 caaatcagat tgcctccatt gcttacaatg aaagaaccag cgttgacgat gtgatgaatg
     1501 aagcggaaaa agctgttttc aacgtcagcg aaaggcgtct caaacatgat cttcagccga
     1561 tctcttcggt cttaagcgaa tactacgacc gcattgatga cctcgccaag cgtcctgatg
     1621 aattttttgg tgtaccaaca ggttttattg atctcgataa aatgttgagc gggctgcaac
     1681 catcggatct attgatcatc gcgggtagac ccggacaagg caagaccgga ttcttacttt
     1741 cgatcgctaa aaatgcgtcg ctcactcaca agaaacatgt agcaatcttc tcgctggaaa
     1801 tgtccaatga acaggttgtg cagaggttga tcgcgcagga aaccggcatt gactcgcagc
     1861 gtctgcgaac agggaagctg gaagaaaccg aatggcctct ctttacacat gcgattgaag
     1921 tgttctccga tacgcatgtc tatctcgacg acacgcctgc gatcacgccc ttgcaattgc
     1981 ggacgaaatg ccgccgcctg catatggaat ttggactgga cctcgtcatt attgattacc
     2041 ttcagttgat gggcggtgac actcgcaacg ataatcgtgt gcaggaagtc tcttatattt
     2101 cacgcaatct caaagtgttg gcgcgcgagt tgaatgtgcc tgtccttgcc gcagcccagt
     2161 tatcgcgtgc tgtcgagcag cgttctgata aacggcctgt cctttcagac ttgagggaat
     2221 caggctctct tgaacaggat gccgatatcg tcatgttcat ctatcgccct gaccagtatg
     2281 agaaagacac ggtcaagcaa aacgtggctg agatcattgt ggcaaaacat cgtaatggtc
     2341 ctgtggggag cgtagaactc gtattccgta acgcgctggc gaagttcgag aacgccgcga
     2401 cacgaatatt caaaccgaat gaataattat ggtgtaggga ggagcagctg ctcctcccta
     2461 caagacctgc atgaaaacat ttaaaggctt caccgattcc gaaacgttca ctcaactccc
     2521 caatgcgttt tttcatcagt tgatccatca gattgatgac atcgcggagt tgaaagtcac
     2581 tttgttcgtc atgtggcatg tagatcacat ggacggaccg tttcgggcgt tgagcaagat
     2641 ggacttcgat gtgaaagaat tggggcttgg ggccgatgag atcacgcgcg ggctggaaaa
     2701 agcagtaagg agggggagcc tgttgacagt caacgctccg cgagacgcga tggtgtactt
     2761 cctgcttaat tcgccacggg ggagatctgc tattcaggct atcgaaaagg gaaaatggaa
     2821 tccggcagat ggggtttccg ctccgatttt ggaacgccca aatatcttta aattgtatga
     2881 agagaatatc ggcccactga cgccgctgat cgcggacgcg ttgaaggacg cggaacaaac
     2941 gtatgcagat gagtggattg cggaagcgat cgaactggct gtaaaaaaca acaaacgata
     3001 ttggaaatat tgtgaagcga ttttgaagcg atggaaagaa gaaggacggc atggaaaaaa
     3061 agatcagcaa gactctgtcc agagttctga acgatacaca aaaagcgaat tcgcagaata
     3121 tctcgaatct gagtaaccct gccatgcaaa tgtttggaaa accagattgt ccacattgtg
     3181 gcggtgtcgg ctatttgcgc acggatgttc cggtcggcca cgaaaaattt ggaaagcttg
     3241 agccgtgtgt atgtcgcgca agtgatattg ctgcgaacgc gcgccagcgg ttgtatgaat
     3301 tgagcaacct ggagcggttg agtcacctga cctttgaaaa tttcaatcgc tctggaaacc
     3361 caaaagcgga atttatttct cctcaagaag cagccagcct gcacgaagcc tacacatctt
     3421 ctgagacgtt tgcaagagat ctgcagggct ggttattgct ggaaggttcg tatggggggg
     3481 gaaagacgca tttagccgcg gcaattgcca acgcagccgt acagcggggt gttccgactt
     3541 tatttatcac cgtgccagat ttgctggatt ctctacgctt ctcattcagt gatgcgacaa
     3601 cttcctttga gtcgcgtttc gatgatattc gcgacgcggc tctgcttgtg atggatgatt
     3661 ttggaacgca caatgccacg ccatgggcac aggaaaaact attccagatc atcaattatc
     3721 gctatatcaa taaactgcca acggtgatta caacgaatct gattctggat gaagtcgaaa
     3781 gccgcattcg ctcacgcctg caggatgcag actatgtaaa gcatgttcgt atcacatccg
     3841 cagactatcg ccgtccgaaa gagaccagta accccggcat ttcgatgctt tcgtttacag
     3901 atatcaagga aatgtccttt gggaattttg aaacacgcga tgatgaagtc ggcaaggaga
     3961 tcgtaaccac gattgtcaag gaaagacagg atcgatacgg acgaccattc aaggataagg
     4021 aaattgttcg agaaaaagta acgcagcaac acatcaaacg cttgcacgac gcgctggatg
     4081 ccgccgtaca atttgctgaa aacccaaacg gctggctggt ttttctggga ggatcacaca
     4141 gcggaaaaac tcatcttgca gcagcgattg gaaactaccg tttgttgttg ggaggacaag
     4201 caattctggt ggaggtatcc aatctgttgg attatctgaa atcaaccttc cgccccaact
     4261 cagatgtgac gtttgaccgc cgctcctacg agattcgcac tacgcccctc ctgatgctcg
     4321 atgatttgaa ggaaggtggt attagctcag tttgggcaga agacaaattg caccagattt
     4381 tgaaccaccg atattatgcc aatttgccta cggtcatcac ctcaaccctg gaccccgaaa
     4441 gttttgcgaa gagctacccc agtctgtgga ataagatttt agatcccgcc aagtgccgag
     4501 tctgtgtaat tgatatgcca ccttatcgcc gcgctgccaa agtgaaaaag ccagccgtat
     4561 cgaaaaaagt taaataagaa aacgaatagg aacagataga tatcaacagg ctttgggtct
     4621 aaaagacgaa aagcctgttt ttatttcgtc tggaaatcaa aaacttcact gaacgccgtt
     4681 atcacttcct gttttacgcg ttccatggaa ggtggctctg ggaacaaatc cgcaagtgaa
     4741 acgattggct catcctgcaa tccacaggcg atgatgccct cccaatattc catatctgga
     4801 tttacattca atgcaaagcc atgacgtgaa atgccgcgcg catccacttt gacgccgatg
     4861 gccgcgatct tggctggctt tttgcgatct tcgggcttgc agcgcgggca acgcgaatgc
     4921 acatccgcct gaatccacac cccggtcaaa ccgggtcgct gtccagccgg cagacccagc
     4981 cgtgctaacg ctgtgatgag tgttttttcc aaattcctta catagccgac gtaatgggct
     5041 tgaggaatgc gcgagtcaga cctgtcaggt ctcagcgtgt ccaaggtcgc ctcggctgta
     5101 tgcgcggacg aatcaccttg aagttcggca tcctccgaag acctgacagg tcttaatggc
     5161 aacaacggat aacccactaa ctgccccggt ccgtgatagg tgacatcgcc gccgcgatcc
     5221 acccagtgaa t
//
LOCUS       DASTJP010000740         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_954697, whole genome shotgun sequence.
ACCESSION   DASTJP010000740 DASTJP010000000
VERSION     DASTJP010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954697"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFQ23_21565"
     CDS             <1..308
                     /locus_tag="VFQ23_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909251.1"
                     /translation="LILAVCEFLSRLHSDCAQPHQQFESLVETQIKKQLFDLLSYWLK
                     QTNVKISTDIPATVATWAIYGLASHYSHLKKRPALEKFVEDAMPLVAVNLEQFAQPA"
     gene            complement(376..1116)
                     /locus_tag="VFQ23_21570"
     CDS             complement(376..1116)
                     /locus_tag="VFQ23_21570"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9909252.1"
                     /translation="MDINLHQDLIEKNQEYWNRKPLLRTLYRDFYTLISTHLSRLPDG
                     KIVELGSGLGNIHDVIPECIRTDLFPNQWIDQIENAYKLSFADESVSDLILTDVFHHL
                     KYPGTALNELYRVLRKGGKVIMLEPCISALGLVVYGALHDEPIAVTKDIEWFAPETWS
                     PENIDFYAAQGNATRIFVGNKFRLNLKNWKSIKTIRLSAIAYAASGGYSKPQLYPTAF
                     LPLIKRLEKVLDLFPILYATRLLVILEK"
     gene            complement(1128..2561)
                     /locus_tag="VFQ23_21575"
     CDS             complement(1128..2561)
                     /locus_tag="VFQ23_21575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9909253.1"
                     /translation="MSLKTDFEPAGINYQQSRIAHWDSVARKRDSWQGMGRWYHQRME
                     EIYQFLVSPGLRVLEIGCSDGNLLAALHPSRGVGVDFSPEMIRRAKERHPELTFVHAD
                     AHDLSEIDGPFDVIILSDLVNDLWDVQRVLEQLQPLCTERTRVILNFYSRLWQLPLNI
                     ARGLNLATSNLYQNWLTREDMKVLLRLASFEPIRITQEVLWPLPMGGLANRFIVRFWP
                     FHHLAISNLMVARPIPKQRKEPVVSVIVPARNESGNIKAIFERTPRMGRETELVFVEG
                     HSKDDTYESIKREIAAHPSTPSLLFQQTGIGKADAVRLGYSKANGDVLMILDADLTVP
                     PEDLPRFYEALVSGKGEFINGVRLVYPMEKEAMQTLNFFGNKFFSLAFSWMLGQSIKD
                     TLCGTKVLWKTDYERIAANRSYFGDFDPFGDYDLIFGAAKLNLRIVDLPIRYRERTYG
                     STNISRWKHGLLLMRMVAFAARRIKFT"
BASE COUNT          697 a          644 c          631 g          649 t
ORIGIN      
        1 atctgatcct ggcagtctgt gaattcctct cccgcctgca cagcgactgt gcacaacccc
       61 accagcaatt tgaatccctc gtggaaacgc agatcaaaaa acagttattc gatttgttgt
      121 cctactggtt gaaacaaacc aatgtaaaga tctccaccga tatccccgcg accgttgcaa
      181 cgtgggcaat ctatggattg gcatctcatt acagccattt gaaaaaacgc cctgccctcg
      241 aaaagttcgt ggaagatgca atgccgctgg tagcggtcaa tttggagcag ttcgcgcagc
      301 ctgcttaaaa agtaaaaacc tccaggtcaa tttgaccttg gaggttttct gacaaattca
      361 tctataatga tacagtcact tttcgaggat gactaatagt cgcgtggcat ataggatggg
      421 aaacaaatcc agaacttttt caagtctctt aatcaaaggc aggaacgctg ttgggtagag
      481 ttgaggcttg gagtaaccac ccgacgcggc gtaggcaatc gcggagagcc ggatcgtctt
      541 gatacttttc caattcttca agtttaaacg aaatttattg ccaacaaaaa ttcgagtcgc
      601 atttccctgg gcagcataga aatctatatt ttccgggctc caggtctccg gagcaaacca
      661 ttcgatatcc tttgtaactg caatcggctc atcgtgtaac gctccataaa caacaagccc
      721 cagggcgctg atacaaggct cgagcatgat aacctttccg cctttgcgca aaactcggta
      781 aagttcattc aacgctgtac cgggatactt caggtggtga aagacatccg tcaatatcag
      841 atcggaaacc gactcatccg cgaaggaaag tttgtaggca ttttcgatct ggtcgatcca
      901 ctggtttggg aataagtcgg tgcgtatgca ttccgggata acatcatgaa tattgcccag
      961 gccggagcct agctctacga tcttgccatc gggcaatctg ctaagatgcg ttgagatcaa
     1021 ggtgtagaaa tctctataca acgttcggag caaaggcttg cggttccagt attcctggtt
     1081 tttttcgatc aggtcttgat gcagattgat atccaaaata tgtttcctta cgtaaatttt
     1141 atccgacgcg cggcaaaagc aaccatgcgc atgagaagca aaccgtgctt ccagcgtgaa
     1201 atatttgtgg aaccatacgt ccgttcacgg taacggattg gaagatctac aatccttaaa
     1261 ttaagttttg ccgcgccgaa gatcaggtca taatcaccga acgggtcaaa gtcaccaaaa
     1321 taggatcgat ttgctgcaat gcgctcgtag tctgttttcc agagaacttt cgtgccgcac
     1381 aaggtgtctt tgatcgattg tcccaacatc catgagaaag ccaggctgaa gaatttattg
     1441 ccaaagaagt tgagcgtctg catggcttct ttctccatgg gatagaccag gcgaactccg
     1501 ttaataaact ctcctttgcc agaaaccagt gcctcataaa aacgcggcag gtcctcgggt
     1561 gggaccgtaa ggtccgcatc aaggatcatc aacacatcac cgttagcttt ggagtagccc
     1621 agccgcactg cgtccgcttt gccgatgccg gtttgctgaa agagcaggct tggggtcgaa
     1681 ggatgtgcag cgatttcccg cttaatggac tcataagtat cgtcctttga atgcccttcc
     1741 acgaagacca gctcggtctc gcgtcccatt cggggagtcc gctcaaagat ggctttgata
     1801 tttccagatt cgttacgcgc cgggacaatg acggaaacaa caggttcttt tctctgttta
     1861 gggatgggcc gcgcgaccat cagattggat atagccaggt ggtgaaacgg ccaaaaacga
     1921 acaataaaac gattcgccag tcctcccatt ggaagaggcc ataagacctc ctgcgtgatt
     1981 cgaatcggct caaaactagc caatcgcagc aagactttca tatcttcgcg ggtgagccag
     2041 ttttggtaaa gatttgaagt tgccaggttt aggccccggg caatattaag cgggagctgc
     2101 cataacctgc tgtaaaagtt cagaatgacg cgagtgcgct ctgtacaaag aggctgaagc
     2161 tgttcaagga ctcgctgaac atcccaaaga tcgttgacca aatcagacag gatgatcaca
     2221 tcgaaaggtc catcgatttc cgaaaggtcg tgggcatctg catgaacgaa cgtcagctca
     2281 gggtggcgtt cctttgcgcg gcggatcatt tcaggtgaaa aatccacgcc gacgccgcga
     2341 gacggatgga gggccgccag caaattcccg tcactacagc caatctccaa aacgcgcaga
     2401 ccggggctga cgagaaattg atagatctct tccattcttt ggtgatacca ccgccccata
     2461 ccctgccaac tgtcgcgctt gcgtgcaact gaatcccaat gtgcaatacg agattgttga
     2521 tagttaattc cagcgggctc aaaatcggtc tttaggctca tttcctaatg attttatgcg
     2581 gggagccatg ctttgtatag gaggttttgt agctcaaagc c
//
LOCUS       DASTJP010000741         6584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1227575, whole genome shotgun sequence.
ACCESSION   DASTJP010000741 DASTJP010000000
VERSION     DASTJP010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227575"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFQ23_21580"
     CDS             complement(<1..533)
                     /locus_tag="VFQ23_21580"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9909254.1"
                     /translation="MAKDVIMPALGMAQETGTLIQWLKSAGDSVTKGEPLMEIETDKA
                     TVEIEAPASGTLSSITAQAGDVIPVGQRIALILAQGESDASTSAQSSPTPVSSPQSES
                     KPVSVNATPVAARLAAEHNVDISRIKPDGGQIRKEDVLAYLDNHNKPIAISRVLASPK
                     ARRLAHERGLDLKSISGT"
     gene            complement(548..1933)
                     /locus_tag="VFQ23_21585"
     CDS             complement(548..1933)
                     /locus_tag="VFQ23_21585"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9909255.1"
                     /translation="MPIKTFGTMGVDWEERVHFDRLREERLARIKGLLQKSEIGALLC
                     FDMNNIRYITNTTIGTWAIDKLGRFCLLPQNDEPINWDFGIAAKHHQMYCSWFKEGHS
                     RAGISTLRGAMPPGAQRAEDVARKIRIELEERGLHKEPLGVDVIELPVLFALQKEGIT
                     VVDGQQLMQDARVIKTQDEITLLSTACSMVDAAYDELYRFMKPGVRENECVGLVNNVL
                     YSLGSEHVEGVNAISGERCSPHPHIFSDRMLRPGDLAFFDILHSYMGYRTCYYRTLSV
                     GSASQAQVDAYKRCRYYLDVAIDAIKPGVTTADVVKLWPKAEEFGVANEELAFALQYG
                     HGVGLAIWEKPVFSRLVSFDSPEEIKEGMVFALETYWPATDGWSAARIEEQLVVTKDG
                     CEVITRFPAEELMVVGKKYYTATGPLSETRETQSHKNIDYTGVDYGGAGWPDGYVPST
                     HRSDGKGVPAA"
     gene            complement(2071..3057)
                     /locus_tag="VFQ23_21590"
     CDS             complement(2071..3057)
                     /locus_tag="VFQ23_21590"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET9909256.1"
                     /translation="MSERELFFGEAIKEALAEELRRDPTVFMLGEDIAEAGTTFKVLN
                     GLVEEFGTERILDTPISEPGFTGLAVGAAMTGMRPVVDIMFGDFSGLIMDQVANQAAK
                     IHYMSGGKLKVPMVLRTTMGAGRRSAAQHSQSLHAWYSHIPGLKVIVPSTPYDAKGLM
                     KSAIRDDNPVVILEDKMMYKTVKGPVPEEEYTIPIGVADIKREGKDITLIATSSMVYV
                     ALEAAKLLEEIGISAEVVDPRTLVPLDKETLINSVKKTSRAMVIDEGYEQYGVTAELA
                     SVIADGAFYYLDAPVKRMGAMNVPIPFSPALEDLTIPNAKGVFEMAKSLCNK"
     gene            3056..3265
                     /locus_tag="VFQ23_21595"
     CDS             3056..3265
                     /locus_tag="VFQ23_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909257.1"
                     /translation="MGTLVTLHVIAKAMIVRPKQSSVIRELLRRLGLDMPFATNVQGY
                     STSGSSQHLHLAHTAPVGRCRGVQV"
     gene            complement(3270..4265)
                     /locus_tag="VFQ23_21600"
     CDS             complement(3270..4265)
                     /locus_tag="VFQ23_21600"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET9909258.1"
                     /translation="MSQVTTEESMTDIDREQWLHMYEQMAKIRAFEEQVNELYQGAKM
                     PGLAHLYIGEEAIAVGVCEALNREDYITSTHRGHGHCLAKGASIDKMFCELLGKEAGY
                     CHGKGGSMHIADQDTGNLGANAIVGGSSAIATGAAFSSKRLGNGRVAVCFFGEGALGQ
                     GLIYESMNMAQLWKLPVIYVCENNLYNEYTHHSESTAGSMKGRAEAFGIMTVEVDGQN
                     VREVHEVARTVVERARSGEGPVFMLCHTYRYLGHHVGDISREYYRPKEEEMKWRNERD
                     PLKILSGWLIEHQMADTGIFEQIENQVQNEVQSAVEYALNAPFPAPEEVTNHVYV"
     gene            complement(4267..5151)
                     /locus_tag="VFQ23_21605"
     CDS             complement(4267..5151)
                     /locus_tag="VFQ23_21605"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9909259.1"
                     /translation="MANLGFVGLGLMGSRIVNRLLAAGHQVSGYNRTRAKAESLIQAG
                     MQWKDSPREVAEAADITLSMVTDTAALSSITDGANGILAGLSAGKIYVDLSTVSPRLT
                     RDLAERVASTGAEMLDAPVSGSLPAAESGTLIIFVGGSADALEKARPVFESISQKIIH
                     VGTNGQAITTKIAINLNLPIQLIGIFEGVLLAERSGIPRAEALDALLNSVIASTSLKY
                     RAPFMLKMPEEVWFSASMMKKDIDLALELGEELGVSLRTAELVNEMLMKAIEMGWGDE
                     DFAVLFKVVEHMSVKSEA"
     gene            5358..6044
                     /locus_tag="VFQ23_21610"
     CDS             5358..6044
                     /locus_tag="VFQ23_21610"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9909260.1"
                     /translation="MAETNPNHARAIGEKLRGVRQERKMSLRDLADRSEISASMLSQI
                     ETGKVFPSVRSLYGIAAALSVSVDYFFPEQVNNNGASQGSPLPSSAGEMTASEMRDAK
                     LRGSGDSATEFPGTIQKSPVVHASSRPTIELKGGVSWSRLTSLAESGAEFLEISYSPG
                     AMSGEHMSHHEGREFGLILDGELVVELGFESYRLGAGDSIIFESTTPHRLSNQGERPM
                     RAIWVVLSKV"
     gene            complement(6056..>6584)
                     /locus_tag="VFQ23_21615"
     CDS             complement(6056..>6584)
                     /locus_tag="VFQ23_21615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9909261.1"
                     /translation="IRYAAPNVTDAEMLEMARKIGDGEWLETLPNGIQTEVGERGARL
                     SMGQRQLVALMRVLLQKPAIFILDEATASIDPFTEWQIQQALNLILKKSTSILIAHRL
                     STVKAADRIVVMEKGSIIEEGNHNGLLAQGGHYATLYNTYFRHQSLDYRPPGMEELLA
                     YKMGLAAGTNQSAES"
BASE COUNT         1521 a         1822 c         1614 g         1627 t
ORIGIN      
        1 gtaccagaga tagatttgag gtcaagtccg cgttcatgag caagccttct tgcttttggc
       61 gatgcaagga ctcgagaaat agcaatcggt ttgttgtgat tgtcgagata agcgagtacg
      121 tcttccttgc ggatctgtcc gccatcgggc ttgatccgtg agatatctac gttatgttct
      181 gcggcaagcc gcgcggcaac gggagtggca ttaacagata caggcttgga ctcgctttgc
      241 ggcgaagaaa ccggcgtcgg tgacgattga gcggatgtac ttgcatcgga ttcgccctgt
      301 gcgaggatga gcgcgatccg ctgcccgacc ggaatcacat ctccagcctg agccgtgatg
      361 cttgataggg ttccactggc aggcgcctcg atctcgaccg ttgccttgtc ggtttcgatc
      421 tccatcaacg gctcaccttt ggtgacagag tcacccgcag atttcagcca ctgaatcagg
      481 gtgccggttt cctgcgccat tccgagcgcg ggcatgatca catcttttgc cacgttgaca
      541 tttcccgtta tgctgccggg acaccctttc catctgagcg atgcgtactc ggaacatatc
      601 catcgggcca acccgcgcca ccataatcca cgccagtgta atcaatgttc ttgtgcgatt
      661 gcgtctcgcg cgtctctgaa agtggacccg tggcagtgta atatttcttg cccaccacca
      721 tcaactcctc ggcagggaag cgcgtgataa cttcacagcc atctttggtg acaactaact
      781 gctcctcgat gcgtgccgca gaccagccat cggtggcggg ccaataagtt tcaagggcaa
      841 agaccattcc ttctttgatc tcctccgggc tgtcgaagga gacgaggcga ctaaagacag
      901 gtttttccca aatcgcaaga ccaacgccat gcccatattg aagagcaaag gcaagttcct
      961 catttgccac cccaaactct tcagccttgg gccacaactt cacaacatct gcagtagtaa
     1021 cgccaggttt gatcgcgtca attgcaacgt ccagataata tcgacaacgt ttgtacgcgt
     1081 ccacctgggc ctgggaggca ctgcccacgg aaagtgtacg atagtaacaa gtgcggtaac
     1141 ccatataact gtgcagaatg tcgaagaagg caagatcgcc cgggcgtaac atgcggtcgc
     1201 tgaagatgtg tggatgcgga ctgcatcgtt cgcccgagat ggcattcacg ccttccacgt
     1261 gttccgagcc gagactgtac aagacgttat tgaccaaacc gacacattcg ttttcgcgaa
     1321 cgcctggctt cataaaacga tacagttcat cataagctgc atcgaccatg ctgcaggcag
     1381 tggaaagcag cgtgatttca tcctgtgttt tgatgacgcg cgcgtcctgc atcagttgtt
     1441 gaccatccac aaccgtaatg ccttcttttt gcagagcaaa aagcacgggc agctcgatca
     1501 cgtccacgcc gagtggttct ttgtgaagtc cgcgctcttc cagctcgatg cggattttac
     1561 gcgctacatc ttctgcgcgc tgtgcaccag gaggcatcgc accacgcagg gtggagatgc
     1621 cagcccggga atgtccttcc ttgaaccagg aacaatacat ttggtgatgc ttcgccgcga
     1681 taccaaagtc ccagttgatc ggttcatcgt tttgcggcag cagacaaaaa cgaccgagtt
     1741 tgtcaatcgc ccaggtacca atggtcgtat tggtgatgta gcggatgttg ttcatgtcga
     1801 agcagagcaa cgcgccgatc tcagactttt gaagcaatcc tttgatgcgc gcgagccgtt
     1861 cttcgcgcag gcgatcaaag tggacgcgct cctcccaatc gacacccatg gttccgaaag
     1921 ttttgatagg catggttcct ccgatttacg cttggtaacc gttggtcgag acgcaagcag
     1981 tcgagaccaa cgcgtttcaa aagtaccttt gttacatgaa ttgtcttaga catcgtggtc
     2041 tcgatacctt cgtactcgac caccaattgc ttacttatta caaagcgact ttgccatctc
     2101 aaacacgcct tttgcgttcg ggatggtcaa atcctccaaa gcaggtgaaa acggaatcgg
     2161 gacgttcatt gcacccatcc gcttgacggg tgcatcaaga taatagaagg cgccatcggc
     2221 gatgacagag gcgagttcgg cagtgactcc gtattgttca tagccttcat caatcaccat
     2281 cgcgcggctg gttttcttta cagaatttat tagagtttcc ttgtcgagcg ggacaagcgt
     2341 gcgcgggtcc acaacctctg cgctgatgcc gatctcttcc agtagtttcg cggcttccag
     2401 cgcaacatac accatgctgc tggttgctat gagcgtgata tctttgcctt cgcgttttat
     2461 gtcagcgacg ccaatgggta tcgtatattc ttcctctggc acaggtccct ttaccgtctt
     2521 gtacatcatc ttgtcttcga gaatgacgac cgggttatca tcgcgaatgg cgctcttcat
     2581 caatcccttg gcatcatagg gagtggatgg cacaatcact ttgagcccag ggatatggct
     2641 gtaccatgca tgcagagatt gcgagtgctg cgcggccgaa cggcgtccgg cacccatggt
     2701 tgtgcgcaac accatcggga ccttgagctt tccaccggac atgtaatgaa tcttcgccgc
     2761 ttgattcgcg acctgatcca tgataagtcc gctgaagtcc ccaaacatga tgtccaccac
     2821 cggacgcatc cccgtcatag ccgcaccgac agcgagcccg gtaaaaccag gttccgagat
     2881 gggtgtatcc agaatccgtt ctgtgccaaa ctcttcaacc aaaccattca ggaccttaaa
     2941 tgtcgtgcct gcttcggcaa tgtcttcacc gagcatgaac acagtggggt cgcgtcgcaa
     3001 ctcctctgcg agcgcttctt taatggcttc gccgaaaaac agctcgcgtt cagacatggg
     3061 taccctcgtt acgcttcatg tcattgcgaa ggcgatgatc gtccgcccga agcaatcttc
     3121 tgttataagg gaattgcttc gtcgcctcgg tctcgatatg cccttcgcaa caaacgttca
     3181 gggctactcg accagcggct cctcgcaaca cttgcacctg gcgcacaccg ccccggttgg
     3241 tcggtgccgg ggagtgcagg tgtgacatac taaacataaa catgatttgt tacctcttcg
     3301 ggagcgggga agggggcatt cagtgcatac tcaacagcag attgcacttc gttttgtacc
     3361 tgattctcaa tctgctcgaa gatccctgta tctgccatct gatgctcgat caaccagccc
     3421 gataagatct tgagaggatc tcgttcgttg cgccacttca tctcttcttc tttagggcga
     3481 taatattcac ggctgatatc gcctacgtga tgaccgagat agcggtaggt atgacacaac
     3541 ataaatacag ggccttctcc gcttcgtgcc cgctccacta cggttcgagc aacttcatga
     3601 acttcacgca cattttgacc atccacttca acggtcatga tcccaaaggc ttccgcgcgc
     3661 cccttcatgc ttccggctgt cgattcggag tgatgagtat attcgttgta taaattattt
     3721 tcgcatacat agataacggg cagtttccac aactgagcca tattcatgga ctcatagatc
     3781 aacccttgcc ctaaggcccc ttccccaaag aagcacacag cgacgcgtcc attgcctaag
     3841 cgcttacttg aaaatgctgc accggttgca atcgctgagc tcccgccaac gatggcattc
     3901 gcacccaaat tgcccgtgtc ctgatcggcg atgtgcatcg agccgccctt gccatggcag
     3961 taccccgcct ccttaccaag caattcacaa aacatcttgt caatggatgc gcccttggcg
     4021 aggcaatgcc catgaccgcg gtgggtgctg gtgatgtaat cttctctgtt cagcgcttca
     4081 caaactccga ctgcgatcgc ttcctcaccg atgtataaat gtgccaggcc cggcatcttc
     4141 gcgccctgat acaactcatt gacctgctct tcaaaggcgc gaatcttggc catttgttcg
     4201 tacatgtgaa gccattgttc gcggtcgata tccgtcatgg attcctctgt tgtcacttga
     4261 gacatgtcac gcctcgctct tcacagacat atgctcaacc actttgaaga gcactgcaaa
     4321 gtcttcatcg ccccatccca tttcaattgc tttcatcaac atctcattaa ccagttccgc
     4381 cgtccgcagg gagacaccca actcttctcc aagctcgagg gcaagatcaa tgtctttttt
     4441 catcatcgag gcgctgaacc acacttcttc cggcattttc aacatgaagg gcgcgcgata
     4501 tttcagcgac gtggatgcaa tcacgctgtt gagcaatgca tccaacgcct cagccctcgg
     4561 gatgccgctc cgttctgcca acaggacacc ctcaaagatt ccgatcagtt gaataggcag
     4621 gttcaaattg atggcaatct tggtcgtgat tgcttgtcca tttgtcccga cgtgaataat
     4681 tttctgactg attgattcaa aaacaggtct ggccttttcc agggcgtctg cgcttccccc
     4741 aacgaagatg atcaacgtgc cagactctgc ggctggcagg ctccccgaca ccggagcgtc
     4801 caacatctcg gctccggtag aagccacacg ctccgcgaga tcgcgggtga ggcgcgggct
     4861 gaccgtgctc aaatccacat atattttgcc agccgagagc ccggccaaaa ttccgttcgc
     4921 cccatcggtg atggacgaca aagccgccgt atccgtgacc atactcaacg tgatatcggc
     4981 ggcttctgcc acttcgcgtg gggaatcctt ccactgcatt ccagcctgaa tcaatgattc
     5041 ggcctttgct cgcgtacggt tgtagccaga cacctggtgc ccggctgcca gcaaacgatt
     5101 tactattcga ctgcccatca agcccaaacc gacaaagccg agattagcca ctgaggatcg
     5161 ctcccgaata gaatgttcaa cgatggactt aaatttaact gaacagatta taatggaatg
     5221 ggcgtttttg tcaatctata ctgaacagaa gaaccaaaat ttaagaattc atgtacgatt
     5281 ttcgcttgct ttgttcactg tgattgaata taatccaatt attcacaaat atccctcaat
     5341 cacactatag gaagaagatg gctgagacaa atccaaacca cgcccgggca atcggcgaaa
     5401 aacttcgagg agttcgccag gaacgaaaaa tgagcctgcg tgatttggct gatcgatctg
     5461 agatcagcgc cagtatgttg agccaaatcg agaccggaaa ggtttttcct tccgtacgat
     5521 ctctgtatgg gattgccgct gcgctctcgg tatccgtgga ttattttttc ccggaacaag
     5581 ttaataacaa cggtgcttct cagggaagcc cgcttcccag cagcgcggga gaaatgaccg
     5641 ccagcgaaat gcgtgatgca aaattacgag ggtctggcga tagcgctacc gaattcccag
     5701 gaactataca aaaatcgccc gtcgtacatg caagctcacg tccaacgatt gagctgaaag
     5761 gcggtgtttc ctggtccagg ttaacatcgc tggctgaaag tggcgcggaa tttttggaaa
     5821 tcagttatag ccccggcgct atgtctggtg aacacatgtc ccatcacgag ggtcgggaat
     5881 ttggattgat cctggatggt gaattagtag ttgagttggg atttgaatcg tacaggctgg
     5941 gcgcggggga tagcattatc tttgaatcaa ccacgcctca tcgtttatcc aatcagggtg
     6001 aaagaccaat gcgggcgata tgggtggtgt taagtaaggt gtagcagaaa atcaattaac
     6061 tttccgctga ttgatttgtc ccggcggcca gtcccatttt atacgcaagc aattcttcca
     6121 tcccaggggg tcggtaatcc agtgattgat gcctgaagta ggtattgtac aacgtagcat
     6181 agtgcccacc ctgcgcaagc aggccattgt gattcccttc ttcaataatt gagccttttt
     6241 ccattacgac gatccggtcc gctgctttga cggtggaaag acgatgcgcg atcagaatgc
     6301 tagtggactt tttcaggatc aaattcaacg cctgctggat ctgccattcg gtgaacgggt
     6361 caatactggc tgtcgcttca tccaaaatga agatggcagg tttctgcaac aagactcgca
     6421 tcaacgccac aagctgtcgt tgccccatcg acaagcgcgc gcctctctct ccgacctcgg
     6481 tctggattcc gtttggaagc gtttccagcc attcgccgtc tccgatctta cgcgccatct
     6541 cgagcatctc ggcgtcggtc acgttcggcg cagcgtaacg gatg
//
LOCUS       DASTJP010000742         5476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1181355, whole genome shotgun sequence.
ACCESSION   DASTJP010000742 DASTJP010000000
VERSION     DASTJP010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181355"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFQ23_21620"
     CDS             complement(<1..664)
                     /locus_tag="VFQ23_21620"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET9909262.1"
                     /translation="MTATEFLKRLRALRAPSVAKSHATLAADNEDEILGVRMGQVFAL
                     AKEFMSMPLDEVEKMLESPIHEMRVGAVSIMDFQARHKKTTEQRKKELFNLYIKRHDR
                     INTWDLVDRSAPYIVGGYLFDKKRDPLYKLARSKKMPERRTAILGTGYFIRQKDVDDT
                     FKIGEMLLEDKEDLIHKAVGWMLRAAGDVDKKQLIAFLDKYAATMPRVALRYATEHFD
                     KKQ"
     gene            complement(683..1267)
                     /locus_tag="VFQ23_21625"
     CDS             complement(683..1267)
                     /locus_tag="VFQ23_21625"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9909263.1"
                     /translation="MRKLIVAEFITLDGVIQAPGGADEDTDGGFIHGGWTHPYWHDDI
                     GAHFFQAMTEADTLLLGRKTWQGHGAAFDPMPEGDPFGDVMKSKKKYVVSTTLKSASL
                     WRNSTIISENVLEEVRKLKDQPGQNIVLDGSSVLVHTLIENDLVDEYFLHVYPLVLGS
                     GKRLFPEGKRVNLKLIQSTPLPTGVVFQQYQPVS"
     gene            complement(1383..1841)
                     /locus_tag="VFQ23_21630"
     CDS             complement(1383..1841)
                     /locus_tag="VFQ23_21630"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9909264.1"
                     /translation="MKKITPFLWFDTQAEEAMNYYVSIFKNSKVLNVQRQGPDGPVFT
                     VTFELDGQEFMGLNAGPRFKFNESISFFVNCENQAEVDEYWNKLISDGGEESMCGWCK
                     DKFGLWWQIIPRQLGELMGDPDPEKSQRVMQAMLQMQKIIVSDLERAHSG"
     gene            complement(1900..2514)
                     /locus_tag="VFQ23_21635"
     CDS             complement(1900..2514)
                     /locus_tag="VFQ23_21635"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9909265.1"
                     /translation="MGKVVFNMTVSLDGFVAGPNDNPENGLGDGGDALFNWYFSGDTE
                     VPISDGNMVLKVSSQSAELLKQSFETYRAGVWGRKTFDIAHAWGGHPPGSPAFIVTHH
                     VPQEWVYEGSPFTFVTDGVESAVRQAKDAAGDKDVVICTASILQQCLNAGLMDEIHLD
                     IAPLLLGKGVRLFDHLNIEPVNLERIRVVEAPGVTHFGFRVVKN"
     gene            complement(2530..3324)
                     /locus_tag="VFQ23_21640"
     CDS             complement(2530..3324)
                     /locus_tag="VFQ23_21640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9909266.1"
                     /translation="MKSVTSKDGTTIAFDQFGAGPVIILVDGALQYRAFDQGMAALAD
                     LLSLHFTVIHYDRRGRGDSTDTQPYAIEREIEDIEAIIDEVGGSASLYGISSGAALAM
                     HAAIKLGNKVKKLAMYEAPYNDEETAQQTWKEYTKKLAELLADGRNGDAVGHFMLLVG
                     ASPEDVEGIRQTPMWPLWESIAPTLAYDHIAAIGEDGKVPVEEATRVAVPTLVMDGSA
                     SFPFMHTTAISLAEAIPNAQHRSLEGQTHEVQAKSLAPVLIDFFKS"
     gene            complement(3351..4193)
                     /locus_tag="VFQ23_21645"
     CDS             complement(3351..4193)
                     /locus_tag="VFQ23_21645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9909267.1"
                     /translation="MKTVTSKDGTTIAFDQSGKGPTVIVVTGALGTRSHLTSDSLAGL
                     LSKHFNVIDYDRRGRGDSTDTQPYAVQREIEDIESLIDAAGGSAFLFGMSSGGVLALE
                     AARELGSKVGKLALYEPPFIIDDSRPPLPTDYVEQLNAAIDAGRPGDAVGIFMTKALL
                     IPDEFVTMMRTAPMSETFSEGSGARPPEWSNMEKVAHTLAYDGLIVRDFLAGKPLPAN
                     RWSAVTAPTVVIVGGNSEPFFHDGAQALVNDMPDARRVILDGQDHAVSSEALAPLVIE
                     FFKS"
     gene            complement(4210..4695)
                     /locus_tag="VFQ23_21650"
     CDS             complement(4210..4695)
                     /locus_tag="VFQ23_21650"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9909268.1"
                     /translation="MASKNKTTILVEPGKQELSITREFDAPRELVYKAHIDPDLYVQW
                     LGPRGYEMILETFEPHSGGRYRYIHRDKNGGEFGFHGVFHEISEDLMIQTFEFEGLPE
                     RGHVVLDTMKLEELPGGRTRATIQSVYQSVADRDGMVQSGMEHGVNEGYERLDELLAS
                     M"
     gene            complement(4697..5032)
                     /locus_tag="VFQ23_21655"
     CDS             complement(4697..5032)
                     /locus_tag="VFQ23_21655"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9909269.1"
                     /translation="MARTPTTHDPFNAVAEPKRRQVLQTLGTNELSVNELVRKLGWTQ
                     PMVSKHLKVLKQVGLVSERRVGRLRMYRVNAERLKPIFDWVSPFELFWIDRFERLDEV
                     LQTMKKKEK"
     gene            complement(5173..>5476)
                     /locus_tag="VFQ23_21660"
     CDS             complement(5173..>5476)
                     /locus_tag="VFQ23_21660"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9909270.1"
                     /translation="YAKFVGEGFRHHNPYFEGSAEALEVGMQENADQNPEKTLDVKQV
                     LAEGALVAVYSEVHHNRGDRGAAVMHLFRFENGRIVELWDVGQEVPETSPNQYGMF"
BASE COUNT         1313 a         1507 c         1236 g         1420 t
ORIGIN      
        1 tctgtttttt gtcaaagtgt tcggtagcat atcgcaaagc gacgcgtggc attgtggcgg
       61 catatttatc taaaaaggca atgagctgtt tcttatcgac atctccggca gcacgcaaca
      121 tccagcccac tgccttgtga atcaaatctt ctttgtcttc taacagcatc tcgccaatct
      181 tgaacgtgtc atccacatcc ttttggcgaa taaaataccc ggtacccagg atcgctgtac
      241 ggcgttcagg catctttttc gagcgcgcca gtttatacaa gggatcacgc tttttatcga
      301 ataagtaacc gccgacgata tagggcgcag aacgatccac cagatcccag gtgttgatcc
      361 gatcatgccg cttgatgtaa agattgaaca actctttttt tcgctgctcg gtcgtctttt
      421 tatgacgcgc ctgaaaatcc atgatgctga ccgccccaac tcgcatttcg tgaatcggac
      481 tctcaagcat tttttccact tcgtccaaag gcatgctcat gaactctttg gccagtgcaa
      541 agacctgccc catgcggact ccaaggatct catcctcgtt atccgccgcg agagtagcat
      601 gagacttggc cacggagggc gcgcgcagtg cccttaagcg tttgaggaat tcagtcgcag
      661 tcattattga gtcggccttt tattaggaga ctggttggta ttgctggaac accacgccag
      721 tcggaagcgg ggttgattgg ataagtttca ggtttacgcg ctttccctcc ggaaataatc
      781 tctttccact tcctaacacg aggggataaa cgtgcaggaa gtattcatcc accaagtcgt
      841 tctctatcag ggtatggacc agcacgctac tgccgtcaag tacaatattt tgacccggct
      901 gatccttcaa tttacgaact tcttcaagga cattctcgct gatgatggtc gaatttcgcc
      961 agagggaagc ggatttcaaa gtagtggata caacgtactt tttcttactt ttcatcacat
     1021 cgccgaatgg atcaccctct ggcatcgggt cgaacgccgc accatgaccc tgccatgttt
     1081 tccgtcccag taacaacgtg tccgcttctg tcattgcctg aaagaaatga gcaccaatgt
     1141 catcgtgcca atagggatgc gtccagccac cgtggatgaa gccgccatcg gtatcttcat
     1201 ccgcgcctcc cggtgcttga atgacgccat ccaaagtaat gaactcagcc acaattagtt
     1261 ttctcatatt tgctccgtat ttgaaagatt actgattatg acacaacatg cagagtccat
     1321 gggcggtccc ttcggggctg gacaataagc aacgttctca ttaccgcaaa ctatcctgtc
     1381 cattagccac tatgtgccct ctccaggtct gagacgatga tcttttgcat ctgcaacata
     1441 gcttgcatga cacgctgaga cttttcggga tcggggtcac ccatcaactc gcctaattgc
     1501 cgaggaatga tttgccacca caaaccgaat ttgtctttgc accagccaca catactttct
     1561 tcgccaccat cggaaatcaa tttgttccag tattcatcca cttccgcctg gttttcacag
     1621 ttgacgaaaa aggaaatcga ttcgttgaac ttgaaacgcg gacctgcatt taatcccatg
     1681 aattcctgcc catccagttc gaaggtcaca gtgaacacag ggccatccgg tccctgccgc
     1741 tgaacgttta ggaccttgga attcttgaag atggaaacat agtagttcat cgcctcctca
     1801 gcctgcgtat caaaccataa aaacggagta atttttttca ttgtagtttc ctttgtattg
     1861 atatattttt tgctgcttgt tcttcataaa catagaaaat cagtttttca cgacgcggaa
     1921 tccaaagtgc gttacaccgg gcgcttcaac tactcgaatg cgttccagat tgacgggctc
     1981 gatattcaga tgatcaaata agcgcacgcc ttttccgagc agaaggggcg caatatcgag
     2041 gtggatctcg tccatcaagc ccgcattgag gcactgctgc aaaatgctgg cagtacatat
     2101 gacgacatct ttatctccag cagcgtcttt cgcctgccga actgcgcttt ctacaccgtc
     2161 ggtgacaaaa gtgaaaggcg acccttcata aacccattcc tgcggaacat ggtgagtgac
     2221 aataaaggca ggcgaaccag gtggatgtcc accccaggca tgagcaatat caaacgtttt
     2281 gcgcccccat accccggccc tatacgtctc aaatgactgc ttgagaagtt cggcgctttg
     2341 cgatgaaacc ttgagcacca tgttgccatc cgagatcgga acttcggtgt caccgctgaa
     2401 gtaccaattg aataaggcgt caccgccatc ccccagccca ttctccggat tgtcattggg
     2461 accagccaca aatccatcca gcgatacagt catattaaaa actacttttc ccatttcttt
     2521 cgcctttcat caggatttga agaaatcgat cagcaccgga gccagcgatt ttgcctggac
     2581 ttcatgtgtc tgaccttcca ggctgcgatg ctgtgcattg gggatcgcct ctgccagcga
     2641 tatggctgta gtgtgcatga agggaaagct tgcactgcca tccatgacaa gcgtcggtac
     2701 agccacgcgg gtcgcttctt cgaccggaac cttaccatcc tcaccgatgg ctgcaatatg
     2761 atcataggca agcgtaggcg caatcgactc ccataggggc cacatgggtg tttggcggat
     2821 tccttccaca tcctccggcg aggctccgac caacagcatg aaatgcccta cggcatcgcc
     2881 gttgcggcca tcggctaaga gctcggccag ctttttggta tattctttcc aggtctgttg
     2941 ggcggtttcc tcgtcgttat agggcgcttc atacatcgcc agtttcttca ccttattacc
     3001 gagcttgatc gctgcatgca tagccagggc ggcgccagac gaaatgccat agagggatgc
     3061 tgacccgccc acttcgtcta tgatcgcttc aatatcttcg atctctcgtt cgattgcata
     3121 gggctgtgta tctgtgctat cgcctcgtcc gcggcgatca taatgaatga cggtgaagtg
     3181 cagtgataac aggtctgcca atgcagccat gccctgatca aaggcacggt actgcagtgc
     3241 gccatccacc aggataatta caggtcccgc tccgaactga tcaaatgcga tcgtcgttcc
     3301 atcctttgaa gttacgcttt tcataagagc ctttcgctgt aggtcttaac tcaactcttg
     3361 aagaactcaa ttaccaatgg cgcgagagct tcagaagaca ctgcatgatc ctgcccatcc
     3421 aaaatgacgc gccgagcatc cggcatatcg tttacgagag cttgtgcacc gtcatgaaaa
     3481 aatggctcac tatttccacc gacaatcaca accgtcggtg ctgtgacggc cgaccagcga
     3541 ttggccggaa gcggtttgcc cgcgaggaaa tcgcggacaa tcaggccgtc ataggccaac
     3601 gtatgcgcga ccttttccat attcgaccac tcgggaggcc ttgcgcccga cccctcgcta
     3661 aaggtctcgc tcataggagc cgtcctcatc atggttacaa attcgtcggg gataagcaaa
     3721 gccttcgtca tgaaaattcc aactgcatct cccgggcggc ccgcgtcaat ggcggcattc
     3781 agttgctcga cataatctgt cggcagcggc gggcggctat catcgataat aaagggcggc
     3841 tcataaagag cgagtttacc gactttactc cctaactctc gtgcagcttc caaagcaaga
     3901 accccaccgg atgacattcc aaagagaaaa gcggatccac cagccgcatc gatcagggat
     3961 tcgatatcct ctatttcccg ctgaactgca taaggctgcg tatcggtgct atcgccgcgt
     4021 ccacggcggt cataatcaat tacgttgaaa tgcttagaaa ggagaccagc cagtgaatca
     4081 gaggtcaaat gtgaacgcgt cccaagcgca cccgtcacga ctataacggt tggtccttta
     4141 ccggactgat caaatgcaat cgtagtacca tcttttgaag ttactgtttt catcgaaaat
     4201 ctctccgtat tacatacttg caagaagctc atcaaggcgc tcgtacccct cgttgacgcc
     4261 gtgctccatc ccagattgga ccattccatc tcgatctgcg acggattgat agacggactg
     4321 aatcgtggcc cgagtgcgcc cccctggcag ttcttccagt ttcatcgtgt ctagaacaac
     4381 atgtccacgt tcggggagtc cttcaaactc aaacgtttga atcatcagat cttcagagat
     4441 ttcatggaag acgccatgaa atccgaattc acctccgttt ttatcccggt ggatgtagcg
     4501 gtacctgcct ccactgtgtg gttcgaacgt ttcgagaatc atttcataac cgcgtggccc
     4561 caaccactgc acataaagat caggatcaat gtgcgctttg tagaccagct cgcgcggtgc
     4621 atcaaattct cgggtgatcg agagttcctg tttgccaggc tcaaccagga ttgtggtttt
     4681 atttttcgaa gccatattat ttctcctttt ttttcatagt ctgcagcact tcatcgagtc
     4741 gctcaaaccg atcgatccaa aatagctcaa agggcgagac ccagtcaaag atgggcttga
     4801 gccgttccgc attgactcga tacattcgta agcgtcccac tctccgttca ctcaccagcc
     4861 caacctgttt caacaccttg aggtgcttcg acaccattgg ctgggtccaa cccagttttc
     4921 taaccagctc gttgacggaa agctcgttgg tgccaagcgt ttgaaggact tgtcgccttt
     4981 ttggttccgc caccgcgtta aacggatcat gtgttgttgg ggttcgtgcc atgggcactc
     5041 attatattcc tatataggaa tatgtcaatg tcttatttta gaatgggtgt tctaatttgt
     5101 caagaggaag ttgataaatt cattccctgt aaatccaccc ttttagcaca gcacataagc
     5161 tttcaatacg aactaaaaca tgccgtattg atttggggac gtttccggca cttcctggcc
     5221 aacgtcccat aattccacga tccgtccgtt ctcaaatcgg aagaggtgca tcacagctgc
     5281 accacgatcg cccctgttat gatgcacttc ggaataaacg gcgacaagcg cgccctccgc
     5341 aagaacctgt ttcacatcca gggtcttctc cgggttttga tctgcgttct cctgcatccc
     5401 aacctcaagg gcttctgccg acccttcaaa atatggattg tgatgccgaa atccctcccc
     5461 cacaaacttt gcgtac
//
LOCUS       DASTJP010000743         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_699393, whole genome shotgun sequence.
ACCESSION   DASTJP010000743 DASTJP010000000
VERSION     DASTJP010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699393"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFQ23_21665"
     CDS             <1..358
                     /locus_tag="VFQ23_21665"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9909271.1"
                     /translation="DGLSIGILSSLKGVGYCTPEQPCPVVTGQDAEVASVKSIIAGEQ
                     MYTVFKDTRVLAKQAAKMADQALKGQTVEINDTTTYDNLVKVVPSYLLTPISIDINNY
                     QKELVDTGYISADDLK"
     gene            469..2010
                     /gene="mmsA"
                     /locus_tag="VFQ23_21670"
     CDS             469..2010
                     /gene="mmsA"
                     /locus_tag="VFQ23_21670"
                     /inference="COORDINATES: protein motif:HMM:NF040905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9909272.1"
                     /translation="MADVILEMRNITKTFPGVKALNNVSFSVTRGEIHALVGENGAGK
                     STLMNVLSGVYPYGTYEGEIYFQGQECRFRDITESEKVGLVIIHQELALIPFLSVAEN
                     LFLGNERAKNGVIDWNESISKTAELLERVGLHESPNTLITDMGVGKQQLVEIAKALAK
                     EVKLLILDEPTAALNETDSEKLLQLLLQFKSHGISSILISHKLSEVSKVADSITILRD
                     GATIETLDCQKDTITEDRIIRGMVGRDLTHRFPPREKNIGEVVFEIRDWNVYHPLHED
                     RKVSTDVNIHVRKGEVVGLAGLMGAGRTELAMSIFGHAFGTRISGQAYRNGKEVDIST
                     IDKAISNGIAYATEDRKAYGLVLIQQVKNNITLANLEEISRRQVINEPQEMKVTSEYR
                     DNLNIKCSSIQQLALNLSGGNQQKVVLSKWLFASPEILILDEPTRGIDVGAKYEIYTI
                     INRLVSEGKGIILISSELPEILGVCDRIYVMRGGKIVGEVPASEATQEVIMKYIMRKQ
                     EVTSQ"
     gene            2052..>2881
                     /locus_tag="VFQ23_21675"
     CDS             2052..>2881
                     /locus_tag="VFQ23_21675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909273.1"
                     /translation="MTTLSGLFRNNIRDYGMLIALIAVMVFFQIRTDGVLMKPVNLTN
                     LVLQNSYVITMAMGMLLIIITGGIDLSVGSVVAVIGALAGVLIVRYDVNWVVVVVASL
                     LLGAAIGAFQGYWVAYLKIPAFIVTLAGMLIFRGLTIALLRGESVGPFPRGFQNISSG
                     FIPDFFQNEGLHITTLLIGVLLSVLLIFLDARGRKNQQKYGLEVTPFYFFVIKNIAIT
                     LAVMSFCYLMASYKGLPNVLVLLFALITFYAFITSSSVIGRRIYAMGGNEKAARLSGV
                     "
BASE COUNT          762 a          631 c          732 g          756 t
ORIGIN      
        1 cgatggtctt agcattggta tcctttcctc gctcaaaggc gttggctatt gcacacctga
       61 acagccgtgc cctgtcgtaa ccggtcaaga tgcagaagtt gcctccgtca aatcgatcat
      121 cgccggcgaa cagatgtata ccgtcttcaa agacacccgt gtgctggcca agcaggccgc
      181 caagatggct gatcaggctc tgaagggtca gaccgttgaa atcaatgaca ccacgaccta
      241 cgacaacctt gtcaaggttg tgccttccta tttgctgacc cccattagca ttgatattaa
      301 caactaccag aaggaattgg tagatactgg ctatatcagt gctgatgatc tgaagtaaga
      361 cgttttatcc atcgtgtgtg agagtttaat gctctcacac acgatgggct taattggcaa
      421 ggttctgcag taaaatcgca ggaaaaattt attaaggacg agaagaccat ggctgatgtc
      481 atcttggaaa tgcgcaacat cacaaagact tttcccggtg tcaaagcgct taataacgtg
      541 agttttagtg tcacccgggg agagatccat gctctggtgg gagaaaacgg agcgggaaaa
      601 tcaaccctga tgaatgtatt aagcggcgta tatccatacg gcacctacga gggagaaatc
      661 tattttcagg gccaggaatg ccggttccgg gatatcactg aatctgaaaa agttggactt
      721 gtcattatcc atcaggaact tgcattgatt ccttttcttt ccgtagctga aaatctgttt
      781 ctcggcaacg agcgcgccaa aaacggcgta attgattgga atgaatccat ttccaaaacc
      841 gctgaattgc tggagagagt gggtttgcat gagtcgccca ataccttgat taccgatatg
      901 ggtgtgggca aacagcagtt ggttgaaatc gccaaggcgc tggcaaaaga agttaagctt
      961 cttattctgg atgagcccac agccgcccta aatgaaacgg acagtgagaa actgctccag
     1021 ttattgctcc aatttaaatc acatgggatc tcctcgattt tgatctctca taaattaagc
     1081 gaagtctcca aagttgccga ttcgatcacc attctgcgcg acggcgcaac gattgaaacg
     1141 ctggattgcc agaaggacac cattacagaa gacagaatca ttcgcggcat ggttgggcgt
     1201 gatctgaccc atcgcttccc gccgcgtgaa aagaatattg gagaagttgt ttttgaaata
     1261 cgtgactgga atgtatatca cccactccat gaagatcgca aagtaagtac cgatgtaaat
     1321 atccacgtcc gcaagggcga agtggttgga cttgcggggt taatgggggc tggaagaact
     1381 gaactggcga tgagcatttt tggccacgcc tttggtactc gtattagcgg acaggcttat
     1441 agaaatggaa aggaagtgga tatcagcacc atcgataaag ccatttccaa cggcattgcc
     1501 tatgcaacgg aagatcgcaa ggcgtatggg ttggtcctga tccagcaggt caaaaataac
     1561 attactctgg cgaatctgga agaaatttcc agacgtcagg tgatcaatga gcctcaggaa
     1621 atgaaggtta ccagtgaata tcgggataac ctgaatatta agtgttccag tattcagcag
     1681 ctggctctta atctttccgg cggcaatcaa caaaaagttg tgctgagcaa atggttattt
     1741 gcaagtccgg aaattctgat cctggatgaa cccacacgcg ggattgatgt tggcgcaaag
     1801 tacgagattt acaccattat caatcgcctg gtcagcgaag ggaaagggat catcctgatc
     1861 tcatcggaat taccggagat tctgggggtt tgcgatcgca tttacgtaat gcgcggcggc
     1921 aagatcgttg gggaagttcc ggcgagtgag gcaacgcagg aagtaataat gaagtacatc
     1981 atgagaaagc aagaggtaac atcacaatga aaaccccggc tgtttatgag acaacaagag
     2041 ataacggaat catgacaact ctgtctggcc tttttaggaa caatattcgc gattatggca
     2101 tgctgatcgc cctgatcgcg gtcatggtgt tcttccagat tcggacagat ggcgtactga
     2161 tgaagcccgt gaacctcacc aatctggttc tgcagaacag ctatgtcatc accatggcga
     2221 tgggtatgtt gttgattatc atcaccggcg ggatcgacct gtctgttggt tcggttgtcg
     2281 ccgtgatcgg cgcgctcgca ggcgtgctga tcgtcagata tgatgtcaat tgggttgtgg
     2341 ttgttgtggc ttccctgttg ctgggcgcag cgattggcgc ttttcaaggc tattgggttg
     2401 cgtacttaaa aatcccggca ttcattgtta ccctggcagg catgttgatc tttcgcggtt
     2461 tgactatcgc tctgcttcgg ggagagtcgg tagggccttt tccgagggga tttcaaaata
     2521 taagttcagg atttattccg gacttttttc aaaatgaagg cttgcacatt accacgcttc
     2581 tgatcggagt gttactttca gtattactga tattcctgga cgcgcgcggc agaaaaaacc
     2641 agcaaaaata tggactggaa gtaactccat tctatttctt cgtcatcaaa aatattgcca
     2701 ttacgctggc agtcatgagc ttctgttatt tgatggcttc ctacaaaggc cttccaaatg
     2761 tgctcgtcct cctttttgca ctgatcacgt tttatgcctt tatcaccagt tcgtcggtga
     2821 ttggtcgccg tatttatgca atgggtggca atgaaaaagc cgccaggctt tcaggtgtca
     2881 a
//
LOCUS       DASTJP010000744         4943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1544886, whole genome shotgun sequence.
ACCESSION   DASTJP010000744 DASTJP010000000
VERSION     DASTJP010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544886"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VFQ23_21680"
     CDS             complement(<1..1154)
                     /locus_tag="VFQ23_21680"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9909274.1"
                     /translation="MTESRSLRVFLCHSSRENSVARELYRQLDTEEWLDVWFVEERLL
                     PSQEWGPEIKKGVKNADALIVLISRNFDAEEKNSYPSWDFVREELHEKVLVLPLLLDE
                     FDLPASMNTWESIIFYPKSKRKVVIQTIIKSLKTHAAQLDISIGKRSGKPVPEKSVQW
                     TPFRWKQLDDGDFEKDTEPELSNSPISTSRQRFKKKLFQGVNHVFLWSASVATLLLLI
                     VCGLTVNYLVKGGGTNSFTAPIVSRALTLVPLPAPTLGVGSVRVSPQDGMRMVYVPAG
                     DFIMGSDDGADDEKPARSVNLDAYWIDQFEVTNRMYKACVDEGKCNPPDYSSAREWLN
                     SELIPFIPLVEPTIIFYADVEFGNYPVSSITWDDASAYCTWVGRRLPTEA"
     gene            1764..3455
                     /locus_tag="VFQ23_21685"
     CDS             1764..3455
                     /locus_tag="VFQ23_21685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9909275.1"
                     /translation="MISITVANATLVLGSHVIFRDLNWEIQHDQKIGLIGPNGAGKSS
                     LFKLITGEHSPEKGGTVIKAKGVSVGYLPQHPEFEPHKTALALALEGNSRVAEIEHEL
                     QRVESKLSDPAVYNNAKSLERVLDAQHKLLEEFESLGGLNYGSRVRDLLQGLGLPESD
                     FEKPISALSGGQKKLIGLARLILARPYVLLLDEPDNHLDMPGKAFLEKLINEYPGAVA
                     IISHDRYILDAIATHIAEIEDGKITTFSGNYTEYIIDKEERLARQEELYQIQQREISR
                     LEMALKRYKTWVIFNDKFATRVHNMEARIARIEKIDRPTLERRKMDLELNGWRGSNQV
                     LEFERVGKSFLPHPSPLPKGEGTHLFVLKDINFLIRHGERVGLIGANGAGKSVLLRLI
                     LGKETPTLGEIRIGPSVKVGYYAQEHETLDFNQSVIDAVRLFGNMSESNAVSFLIRYL
                     FTYQQATQRIGSLSGGERSRLQLALLVLSGANFLLLDEPTNNLDIASAEVLENALNDF
                     NGTVLVISHDRYFLDRTVSRIFALEDAKITEYIGGYSDYAAKAGVHLFGGKQVKA"
     gene            3557..4720
                     /locus_tag="VFQ23_21690"
     CDS             3557..4720
                     /locus_tag="VFQ23_21690"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9909276.1"
                     /translation="MLQEPTSQKTKVCPTCGTRLSENATRCLVCGTELTAKAATKSKK
                     AETIVQASRMPEITLSLPAALGALVTILLIGAAVVYFSLQAGITGTTLSEPTIELTPS
                     ETGTITPTGTEPPTQTPIPTQTELPPFDYTVRPGDTCGGIAVAFEVSVQSLVILNNLP
                     TTCIISENQVLKIPYPTATPAPPPTEIPNEATQTAEACEKVPYTVQANDTLSSISLNY
                     NVPQDAIKFYNGLPTDTVFVGTTILIPLCARFATPGPTPTATLPPPYPAANLLLPADG
                     AAFTLANDVVTLQWASIGTLRENEAYQVTIEDVTSGQGRRLTDFVTDTKFIVPTSFRP
                     NDNLAHVIRWWVTTVRQNGVDDQGQPLFESAGAISEERVFTWVGVAVEGTPNP"
BASE COUNT         1270 a         1280 c         1190 g         1203 t
ORIGIN      
        1 tccgcctctg ttggcaatct tcgcccgacc caggtgcaat aggcagaggc atcatcccag
       61 gtaatactcg aaaccggata gtttccaaac tcgacgtccg cataaaatat gattgttggc
      121 tctaccaacg gaataaatgg gataagttct gagttgagcc attctctggc agaactatag
      181 tctggcggat tgcactttcc ctcatcaacg cacgccttat acatcctatt tgtcacttca
      241 aactgatcga tccaatatgc atcgagattc acagaacggg caggtttttc atcgtctgct
      301 ccatcatcgc ttcccataat gaagtcccca gccggaacgt ataccattct cataccatcc
      361 tgaggtgaca cacgcacact gccaaccccg agagtgggtg ctggcaatgg gacaagagtt
      421 agcgctctcg aaacgatcgg tgctgtgaag gaatttgtgc cgccaccttt caccagataa
      481 ttaacggtca agccgcaaac tatgagtaac agcagtgtgg cgacgctcgc cgaccaaaga
      541 aaaacgtgat ttacaccctg aaataatttc ttcttgaatc gttggcgaga agtgcttatg
      601 ggcgagttag ataattcggg ttccgtatct ttttcaaagt ctccatcatc cagctgcttc
      661 caccggaatg gagtccattg aactgatttt tcaggaacgg gctttccaga acgtttgcct
      721 atcgaaatat caagctgcgc tgcatgagtc tttaggcttt ttataatagt ttgaataact
      781 actttgcgct tgctctttgg ataaaaaatg atggattccc atgtgttcat gcttgcaggc
      841 agatcaaatt catcaaggag caaaggcagg acaagaacct tctcatgcag ttcttccctc
      901 acaaaatccc agctcggata ggaatttttt tcttctgcgt caaagtttct tgaaatcaaa
      961 acaatcagag cgtcagcatt ttttacccct tttttgattt ccggacccca ctcctggctc
     1021 ggaagcaatc gttcttccac aaaccaaacg tccagccact cttcagtatc cagttgacga
     1081 tacagttctc gtgcgactga attctccctg gacgagtgac aaagaaatac gcgtaaggaa
     1141 cggctctcag tcatatagtc tcttcgaata atctatccaa aattatagga tgacccagtt
     1201 agtaaattat gagctataga taagattcga cacggcggaa accgacaaac tcatgatgga
     1261 tcgaggaaat accctggttt caccgctcta tttggataac tgcccatttt cgtagcgctg
     1321 tgcatgattc gtcgtcccag ttaaaacgtc tggggtggga tcagtgttcc aatcgatata
     1381 ggaaccccat gcgtagattc ttggatatta aagtttcaaa ttatggacta agaaattata
     1441 tgccgagatt ggatttgatg ctataatcac gccacgatgc gttacgaacg atctactaca
     1501 tccgtgctgc acattggcgt gcgtggcttt atcagcgaag ggcttcctcg aatcgcgcct
     1561 gtcgctcgaa gatagttcat ttatctgtcg ttaccgccgc aggccgagtt gcctgcggcg
     1621 tttttgttta atttgaatca acaagcctga caggtttcat caagcccttt gttcgttggg
     1681 tttctcacga gagattattt agaacgtctg tcaatgagca ttgaaaacct gtcaggtctt
     1741 atcggtataa ttttggagtt cctatgatct caatcaccgt tgcaaatgcc acacttgtcc
     1801 ttggttctca tgtcattttc cgtgatctca actgggagat tcaacatgac cagaaaattg
     1861 gtctgatcgg gccgaacggt gccggcaagt cttcgctttt caagctcatc accggagaac
     1921 attctcctga gaaaggtggg acggttatca aagccaaagg tgtgtccgtg ggctatctgc
     1981 ctcagcaccc tgaatttgag ccacacaaaa cagctcttgc gcttgcgctg gagggaaatt
     2041 cacgcgtggc agagatcgag catgaattac agcgagtcga aagcaaacta agcgaccctg
     2101 ctgtttataa caatgcaaaa tcgctcgaac gagtcctcga cgcccagcat aaactacttg
     2161 aagaatttga gtctctgggg ggcttaaatt acggctctcg cgtccgagat ttgctgcaag
     2221 ggctggggtt accagaatct gatttcgaga aacccatcag cgcgctgagc ggcgggcaaa
     2281 agaaactgat tgggctggcc agattaatac tggcgcgccc atacgtgttg ttattggatg
     2341 agccagataa ccatctcgat atgccgggaa aggcttttct tgaaaaactg ataaacgagt
     2401 atcccggtgc cgtagcgatc atctcccacg atcgttacat tctggatgcg attgccacac
     2461 atatcgccga gattgaagac ggaaaaataa cgaccttctc gggaaactac accgaataca
     2521 ttattgataa ggaagaacga ctggcgcgtc aggaagagct gtatcaaatc caacaacgag
     2581 aaatcagtcg tcttgaaatg gcgttaaagc gctataaaac ctgggttatc ttcaatgata
     2641 agtttgcgac gcgtgtccat aatatggagg cgcgcattgc acggatcgaa aaaatagatc
     2701 gcccgaccct cgaacggcga aaaatggatc tcgagctcaa cggctggcgt gggtcgaatc
     2761 aggtgttgga gtttgagaga gttgggaagt catttctccc tcaccctagc cctctcccca
     2821 agggagaggg gactcacctc tttgtgctca aggatattaa cttcctcatc aggcatggcg
     2881 aacgggtggg gttaatcggc gccaacggcg caggcaaatc cgtcttgttg agactaatcc
     2941 tgggaaagga aacacctacc ctcggagaga tccggattgg tcccagcgtg aaggttggct
     3001 attacgcgca ggaacacgag acgctggatt tcaatcaatc cgtgatcgac gccgttcggc
     3061 tcttcggcaa tatgagcgaa tcgaacgcgg tatccttcct catccgttat ctgtttacct
     3121 atcaacaagc cacacagcgg atcgggtcac tttcgggtgg cgagcgcagc cggctgcaac
     3181 tagctttgct ggtactttca ggagcgaact ttttattgct cgacgagcca accaataacc
     3241 tggatatcgc ctcggcagaa gtgctggaga acgcgctgaa tgatttcaac ggcacggtcc
     3301 tggtcatctc gcacgaccgc tattttcttg atcgcaccgt cagccggatt ttcgcgctgg
     3361 aggatgcaaa aatcaccgaa tatattggcg gctacagcga ttacgcggca aaggcaggcg
     3421 tccatttgtt cggcgggaag caggtcaagg cgtagaaaga gctgcgtctc ccgtaaaatc
     3481 cgccgatttc gtagggaaaa agcatggaaa ggcggtcaaa cgggtgactt ctgtcaatgg
     3541 tataattccc gcccgtatgc ttcaggaacc cacttcacaa aaaacaaaag tttgccctac
     3601 ctgcggaacc cgcctgagcg agaatgccac gcgctgtctg gtgtgtggaa ccgagctcac
     3661 cgccaaagcc gctacaaagt cgaaaaaagc tgaaaccatt gtccaggcca gccgaatgcc
     3721 tgagatcacc ctcagcctgc ctgccgcgct gggtgctctg gtgactattc tcctgatcgg
     3781 tgcagccgtc gtttacttca gtctgcaggc cggtatcaca ggtacgactc tcagcgagcc
     3841 aacgattgag ctcactcctt ctgaaactgg gacaattaca cccactggca cagagcctcc
     3901 tactcaaaca ccgatcccaa cgcaaacaga actaccgcct ttcgattaca ctgtccgacc
     3961 tggcgatacc tgtggtggga tcgcggttgc ttttgaagtt tcggtgcaaa gccttgtcat
     4021 tctgaataat ctcccgacaa cctgtatcat ttcggaaaat caggttttga aaatacctta
     4081 tccgaccgcc acgcctgcgc ctccgccaac tgaaatcccc aacgaagcca cgcaaactgc
     4141 ggaagcctgt gaaaaggtcc cttacaccgt tcaggcaaac gatacgcttt cgagcatttc
     4201 cctcaactac aatgtgccac aggatgccat caagttttac aatggacttc ccaccgacac
     4261 ggtttttgtg gggaccacga tcctcatacc gctgtgcgct cgattcgcga cgcccgggcc
     4321 aactccgact gctacgctcc cacctcccta ccccgccgcg aacctgctcc tgcctgcgga
     4381 tggagctgcc tttacgctgg caaacgatgt cgtgacgttg caatgggctt cgattggtac
     4441 cttacgcgag aatgaggcgt atcaggtgac gattgaggac gtgacctcgg gtcaggggcg
     4501 ccgcctgaca gatttcgtga ccgacacgaa atttatcgta cctacctcgt tccgcccgaa
     4561 cgacaacctg gcgcatgtca tccgctggtg ggtgacaacc gtccgccaga atggcgtgga
     4621 cgatcagggt cagccgcttt tcgaatccgc tggcgcaatc agtgaggagc gggtctttac
     4681 ctgggtcggc gtagccgtcg aaggcactcc gaatccatag tcgatttgtt cgaactccaa
     4741 ctccccatca ggtttgatgg ggagttttct ttttatctcg acaacccgtt cttcaacatt
     4801 tacaatctca aaagagaaac aatacatgga gagcatatgg acattcgagc ctataaccgc
     4861 gacgcatgga acaaagaagt ggagggtggc gaaaaccgat ggacacaacc tgtcagtcac
     4921 gagatcatcg aaaaggcgag aga
//
LOCUS       DASTJP010000745         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_297707, whole genome shotgun sequence.
ACCESSION   DASTJP010000745 DASTJP010000000
VERSION     DASTJP010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297707"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="VFQ23_21695"
     CDS             complement(<1..1572)
                     /locus_tag="VFQ23_21695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HET9909277.1"
                     /translation="MANKSSNRAKKLLGGIIVIIAILALALPAQAAEPSFQPNFPPTR
                     TPVIEINPDASHHNHANLDAPPPMSALELAVSTCSGGFAGEYPCQGIDFMSRVPLSSF
                     TGNPLSASNLWGYVDLDDNREYAIIGLRNGTGVVNVTDPANPVIVGHIAGVSSQWREV
                     KVYQFWNASASRWDAYAYITTEGSGGGIQIIDLTNLPTSVSLAATWTGVSTSHTDHVS
                     NINYSTNVSNNPNFPPMLYISGSNKGGLRFISLANPTSLTEVGFWTGTYSHDTYAHVF
                     TDARANQCAPGHNPCEVVFSFAGTPGLKIIDVTDKSAPVTLSTFTYPQLGYSHSGWIS
                     SDGNYLFLHDELDEQNFGISGTIRTINITNLTAPFISNVYTTGNAAIDHNGYTIGNKF
                     YFSNYTRGIGILDVTNPNAPVSLAYFDTYPTTNTATFNGAWGIYPYLPSGNILIADIQ
                     RGLIVVREQSSGPTPTPTITPTPSNTPTPSNTPTSTNTPSAGNTGWLSASANAAETNG
                     AGDNNGYEINPSNAFA"
     gene            complement(1836..2336)
                     /locus_tag="VFQ23_21700"
     CDS             complement(1836..2336)
                     /locus_tag="VFQ23_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909278.1"
                     /translation="MTNAKLVMAILVALVLSLTGVVGTTPALAEATTTRESISFPVDT
                     NMFVSCAAGGVGEFIHFTGNMHYTGIFVLDASGGRHFTDINITQNLGGIGLTTGNTYR
                     MAIMGQDHLIAASNDGVAITSLTSFRIIGAGPENDFIIHENGHVTISSDGTEIVGFSN
                     LFVECR"
     gene            complement(2707..3483)
                     /locus_tag="VFQ23_21705"
     CDS             complement(2707..3483)
                     /locus_tag="VFQ23_21705"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9909279.1"
                     /translation="MGKLSGKVAIITGATSGIGKATALLFADEGADVVITGRRAELGA
                     RVEEEIKQTGSRGLFVQADHTIADDCSRVVERTLAEFGRVDILFNNAGIVTRGTAETT
                     SDEVWNETIAINVSAVRRMCKLVIPIMKKQGKGVIVNNGSDWSVVADKDAFPYIMSKG
                     AVAMMTKAMALDHARENIRVNAVCPGDTMVDRWLQSDYFEYTDDASLDAAINAASDYL
                     PMGRFGKPEEIAKAVLFLASDDSSFVTGHLLLVDGGNTAR"
BASE COUNT          879 a          907 c          863 g          964 t
ORIGIN      
        1 agcaaacgcg tttgagggat ttatttcata cccattattg tcgcccgccc catttgtctc
       61 cgcggcattg gcgctggcac taagccaacc cgtgttcccc gcgcttggtg tattggttga
      121 ggtaggtgtg ttcgagggag ttggtgtgtt ggaaggcgtt ggggtgatcg taggcgtcgg
      181 agttggacct gagctctgtt cgcgcacgac gatcagccca cgctgaatat cagcgatcaa
      241 aatgttgccg ctgggcaggt aaggataaat gccccaggca ccgttgaagg ttgcggtgtt
      301 agtagttggg taggtgtcaa agtaggcgag actgacaggc gcgttcgggt tggtcacatc
      361 gaggataccg atgccccgtg tgtaattgga aaaatagaat ttgttaccaa tggtatagcc
      421 gttatggtca atggctgcgt tcccggtcgt atacacgttg ctgatgaacg gtgcagtgag
      481 gttggtaatg ttaatcgtac ggatcgtgcc gctgattcca aaattctgct catccaactc
      541 atcatgcagg aagaggtaat taccgtcgct cgaaatccag ccggagtggc tgtatcccaa
      601 ctgtggatag gtaaacgtac tcagagtcac aggcgcgctt ttatccgtca catcgatgat
      661 cttgaggccc ggtgtgccag caaagctgaa caccacctca caggggttat gcccgggcgc
      721 acactggttg gcgcgcgcgt cggtgaatac atgggcatag gtgtcatgtg aataggtccc
      781 ggtccagaag ccgacttccg tcaggctggt ggggttggca agactgataa accgtaagcc
      841 gcctttgttg gaacctgaga tgtataacat gggcggaaaa ttcggattgt tcgacacgtt
      901 cgtgctgtaa ttgatattgg aaacatgatc cgtgtgagat gtggagacac ccgtccatgt
      961 ggctgccagt gaaacactgg tcggcagatt ggtcaggtcg ataatctgaa ttccaccacc
     1021 agagccttcc gtggtgatat aggcgtacgc atcccagcgg ctggctgacg cattccagaa
     1081 ctgatagacc ttgacctcgc gccattgcga acttacgccg gcaatgtgcc cgacaatcac
     1141 agggttggcc gggtctgtta catttaccac gcccgtgccg tttcgcaaac caataatggc
     1201 atattcgcga ttgtcgtcca gatccacata accccacagg ttggacgcgc ttaacggatt
     1261 gccggtaaac gaactcaatg gcacgcggct cataaaatca ataccctgac acggatactc
     1321 acccgcaaaa ccaccagaac aggtggatac cgcaagctcg agcgcactca taggtggagg
     1381 agcgtccaaa tttgcgtgat tatggtggct tgcatctgga ttgatctcaa tgaccggcgt
     1441 ccgggtgggt ggaaagttcg gttggaagct gggttcagcc gcttgcgcag gtaaggccaa
     1501 tgcaagaatt gctatgatga caataattcc gcccaggagt ttctttgctc tgttgctgct
     1561 tttgttcgcc ataaagtttc cctttctttg ttgagcctgc tgtgatgtcc tatcagagta
     1621 ttagaataac tttaacaaaa ttctattgtg tgcctacttt acagcgttca aatctatctg
     1681 tcaacgggca atttgaaaaa ccatgttgct tttcaacaac tctccctcat gcgtcagccg
     1741 tatataatta gtaagtaatt ttactcatca ctcgacacaa agtgacgggt caagacgtca
     1801 cttgacccgc cttacagcga attcttaacg aaggtttagc gacactcgac aaagagattt
     1861 gagaagccca cgatttcggt accatcagaa gagatcgtga catgtccgtt ttcgtgaatt
     1921 atgaaatcgt tttctggacc tgctccaatg atgcggaagc tggttagact agtaattgca
     1981 actccgtcgt tgctagctgc aattagatga tcttgcccca tgattgccat cctatatgta
     2041 ttaccggttg tcaggccgat tccgcccaaa ttctgtgtga tgttgatatc agtaaaatgg
     2101 cggccgccac tagcatccaa aacaaagatc cctgtatagt gcatgttccc agtgaagtgg
     2161 atgaattctc ctacgcctcc cgctgcacag gaaacaaaca tatttgtgtc gacagggaaa
     2221 ctaatgcttt ccctggttgt ggtggcttcc gccagagcgg gcgtcgtacc tactacgccg
     2281 gtcaggctta acaccaatgc gacgaggatt gccattacta gctttgcgtt tgtcataact
     2341 ctccctaaag tgttcattat gccctctctt cctagtatgg attaagttga cttgacaatt
     2401 gttgagacat actctatttc aatataattg aacggtttta cctccttttc gtggttaggt
     2461 atttagatgt tttgaaataa gaaatatttg atagtagtat tgtaataagt gatttagcgc
     2521 acatcgatat ctcacatcac agaccttatc acttcattca gaccctccct catgttttat
     2581 cccaatactt ctattgaatg aaaatactat acaactttcc aagacagaag tcaactaaga
     2641 ctctattaca ttgaaaaatt cttataggct cctattgagg tgaaatcact aagtggcctt
     2701 cccaacttat cgagccgtat tccctccatc caccagcagt aaatgccctg tcacaaatga
     2761 agaatcatcc gaagccagga ataacacggc cttggcaatc tcttccggct ttccgaaacg
     2821 tcccattggc aaataatcac ttgccgcgtt gattgcagca tccaaacttg catcatctgt
     2881 atactcaaaa taatctgatt gcagccagcg gtctaccatg gtgtcccccg ggcaaaccgc
     2941 gttcacgcgg atattctcgc gtgcgtggtc cagcgccatg gctttcgtca tcatcgccac
     3001 cgcacccttg ctcatgatgt agggaaaagc gtctttatct gccactacgg accaatccga
     3061 gccgttgttg acgatcacac ccttgccttg ttttttcatg attgggatca caagtttaca
     3121 catccgcctg accgctgaga cattgatcgc gatcgtctcg ttccacacct catcggaggt
     3181 ggtttcagcc gtcccgcgtg tgacgatgcc tgcgttattg aacaggatat ccacgcggcc
     3241 gaattcggcc agagtccgct cgacgacgcg ggagcaatcg tcagctatag tatgatccgc
     3301 ctgcacaaat aagccacgcg aacctgtttg tttgatctct tcttcgacac gtgcaccgag
     3361 ctctgctcgt cgtccagtga tcaccacgtc tgcaccttcg tctgcgaata acaacgcggt
     3421 agcttttccg atgccagatg tcgcaccagt aatgatcgca accttacccg ataatttgcc
     3481 catagaattc tccagcaatc aacctatgtc atagcaagcc gtgtcctatt gaacgagggg
     3541 attgcttcgt tacagcttcg ctgctcctcg caatgacaga tgattgcctt gacaatctca
     3601 ttatattcac aat
//
LOCUS       DASTJP010000746         6783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_699527, whole genome shotgun sequence.
ACCESSION   DASTJP010000746 DASTJP010000000
VERSION     DASTJP010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699527"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..231
                     /locus_tag="VFQ23_21710"
     tRNA            145..231
                     /locus_tag="VFQ23_21710"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:181..183,aa:Ser,seq:gga)
     gene            623..787
                     /locus_tag="VFQ23_21715"
     CDS             623..787
                     /locus_tag="VFQ23_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909280.1"
                     /translation="MINQTEPKPYACYDFTTGGIAHHTLKLDFCQANETPDDMPIPIE
                     IPQEQLQTTE"
     gene            complement(860..1486)
                     /locus_tag="VFQ23_21720"
     CDS             complement(860..1486)
                     /locus_tag="VFQ23_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909281.1"
                     /translation="MNDVRQMIYGSLVLFFLVLAFWFSIIYVSACGFTLSCNQADHIV
                     VRTPVPTLIPRAHSNAQPVGAVGEFNQCQVLATDLIGAWVAAGHPEATAFSFADVNGQ
                     PCEGTFAEDVQPLFTENSIWYDGSIGCVSCHNAGQPDRNAGLDLSTYGAMSSADIFGG
                     DDWQNSVLYDVLVNRELVPEGHSTDNTGHAVIVYAGQAMSEPEVTPTP"
     gene            complement(1501..2010)
                     /locus_tag="VFQ23_21725"
     CDS             complement(1501..2010)
                     /locus_tag="VFQ23_21725"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9909282.1"
                     /translation="MRLFRQLFGVFLTLGFLGGVVLLFSYDIIKIEWISFMEIQPAYR
                     PMEDPLPPPAQSIPIEGAISIPGMGAPENPFEVDDASLARGAELYAINCRMCHGPTGG
                     GNGPVAPFLAVKPANLTSDVVQAKSDGSFFLTISNGIDGRMPPLNENLTVPERWDVVN
                     FVRTLKAQE"
     gene            complement(2007..2534)
                     /locus_tag="VFQ23_21730"
     CDS             complement(2007..2534)
                     /locus_tag="VFQ23_21730"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HET9909283.1"
                     /translation="MTETTLLALFDEDKIDPAADAIEKLHEMGIPHENINVISGVPIA
                     HKILGRPHPWTNVSRLALGGAVVGFCIGFFLNYGTPLLYNVPVGGQYITPVPPGMILI
                     FEMTMLFALLSTFLGVFLDSYFPNYRPMEYVPEISDGKIGVFFKYPAGEQEKYVDVLS
                     GFGAESVRPVEVQQL"
     gene            complement(2547..3995)
                     /gene="nrfD"
                     /locus_tag="VFQ23_21735"
     CDS             complement(2547..3995)
                     /gene="nrfD"
                     /locus_tag="VFQ23_21735"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9909284.1"
                     /translation="MAVTSKYFSGAERGHETHHEEEHDPREYLMYDPKPRGEMNDMVM
                     ESMLTTSWKFWVVFAILAVLVATCFVYAWGYMIANGLGVAGVMRPSYWGIFLVNTVFW
                     IGISHAGTFISAILRVMKAEFRRPFTRAAELMTTFGLIQAGASIFMHLGRVWLAYWLF
                     PIPNERQLWPNFHSPLMWDLLAITTYVLGSTMYLFLPLIPDLAMARDRSTGWRKIFYR
                     VLALGFRGTEGEWRHLTTAMNIFAFAIIPVMFSVHTIVSWDFAMAMRPGWSSTVFGPY
                     FVLGALHSGMGAVAVVLFIMRYTMKHMDYFIRKEHFDALGKLMLIVTFAYTYFFFNDY
                     IVQWYGGDKWTDELLHWLEEGPMSWMFYQLLLFNLAIPALTLWNKRIRQTPWLLALIG
                     ILINIGMYWERYLIVPVMLTINRMPFTWKLYEPRIEIFLTIGTFSLFLLFYLAASRLI
                     PLIPVWEVQEGQMTHSMRKVGKANLPTISEFE"
     gene            complement(3999..4784)
                     /locus_tag="VFQ23_21740"
     CDS             complement(3999..4784)
                     /locus_tag="VFQ23_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9909285.1"
                     /translation="MSLSIDLNKMGAKGAIAEEAPGKWGMVIDQDLCTGCQACVAACA
                     MENNLSFVGEEDAGYGRTQHWIRIERFWDGEYPEIKVAGGFQPMLCQQCGHAPCEPVC
                     PVFATPHSNAEELNLQVYNRCVGTRYCANNCPYQVRSFNWRDYHKQPFHANNVDGQLV
                     FPLHNQFNPDVTVRQRGVMEKCTFCIQRIHKAQDTAKSEGREVEDGEFTTACAQACPA
                     NAITFGRIDHPESLVSQLAHQNRGYKHLEELNTLPRITYLEKG"
     gene            complement(4795..>6783)
                     /locus_tag="VFQ23_21745"
     CDS             complement(4795..>6783)
                     /locus_tag="VFQ23_21745"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9909286.1"
                     /translation="GCGLIVRTMQGRAIKVEGNPANPVNLGKTCPRGQITLQGLYNPN
                     RVRGPLRHARGEGLYDANFQDAEPNMNWDEAVQVVAGALQNSGSVAFLLGTAPDHLFD
                     LVTEIAGSTGASAPVRFSVSSMFETRATLSKASENLFGQAAMPFFDMGTSDLVLSFGA
                     NFLETWLSPVAYNRGYSQMRRGNPRVRGYFIQFESRMSQTAAKADEWIPIHPGTEGLV
                     AAAIGRLVAEIRGVAAPAAFEAVDLNAVLAETGLNLETLEHIAHLVAESTSPIAIPGG
                     SALGLSNGLQTAEAILSLNAVVDNLGKPGGVAFSPLSPNQDEYHRPASAQELTEFINR
                     LNSGEIQTLFIHGVNPVYELPTSLGFAEALANVETVISFATFPDETALAADYVFPDHH
                     GLESWGYQRVATGSLQSVLSGAQPVVSPMHDTRATADVLLAASQALPFKDEMEYLQSK
                     FASLVGQADGSFSAPEINTFTAYFQQHGGWWNNAAELLTPTAAGSSNLTVEHAEFVGE
                     GEFFFLPFLSPTLGEAGANKPWLQELPDPTTTVMWNTWVEINPETAHELGLENDDVVK
                     IISDAGEVEAPVYLYPAIHPEVIAMPFGQGHTAYGRYAQGRGANPVALLGAHFNEAGD
                     LTFAGMKVHIEKTGRKQILSRLESRIGVYREGMGEEHP"
BASE COUNT         1763 a         1807 c         1683 g         1530 t
ORIGIN      
        1 gctaaattga tctccaatca ggaaatgcca aagcgcggac ccaagttgac tccgcctcaa
       61 gtgcagatca ttgttgattg gatcaatcag ggcgcgttgg ataattagaa aaacgctatg
      121 gtataatttt gtcgccataa acaaggagag gtcgcgtagt ctggcttagc gcgcacgctt
      181 ggaaagcgtg taacccacaa aggttcgcgg gttcgaatcc cgccctctcc gctgaaaacg
      241 tctcgcaaca agcgaggcgt ttttatttag gttcgcgtgt cccttgaaaa gcacactcga
      301 agggtgcatt gaagggatga tatttgaatt ttaaaatgct tggggccagc gtgtccagac
      361 gctagcccca ggagtatgtt aggcgcgtca ccgcgcatag taactagacg cctacgctca
      421 ctacatgtta cgttgcaaaa aacacatcta tgcttgcctt tttataacaa gtacgccaag
      481 caaaacgcgc ttgcatccat taccaattgc acccttactc attaatgcct ttaattttgg
      541 aactgtaacc tttaagggga atatccctac ttacatccca acttgattag cagtaaaaca
      601 tagtcagata tggaggctcg acatgatcaa ccaaactgaa cctaagccgt acgcttgcta
      661 cgactttacg acaggcggga ttgctcatca tacgctgaaa ctggattttt gccaggcaaa
      721 tgaaacgcct gacgacatgc ccattcccat cgagatccca caagagcagc tacagacaac
      781 tgaatagttc gctacaaata cagacaagaa aaccctcgtt gacgagggtt tttttaattt
      841 cagttatggg agtcgatggt tatggcgtcg gtgttacttc tggttcactc atggcctggc
      901 ctgcatatac gatcacagca tggccagtgt tatcagtcga atgaccttca ggaaccagct
      961 ctcgatttac caatacatca taaaggaccg aattttgcca atcatcgcca ccgaagatat
     1021 cagcagatga catcgcccca taggtggaca aatctaatcc tgcgttgcga tccggttggc
     1081 cagcattatg gcaggaaaca caaccgatcg agccatcgta ccaaatactg ttctctgtaa
     1141 acaacggttg tacatcttca gcaaacgtcc cttcacaggg ttgtccattt acatcggcaa
     1201 aagagaacgc cgtcgcttca ggatgtccag ctgcaaccca ggcgccgatc aagtctgtgg
     1261 caaggacctg acactgattg aattcaccca ctgctccaac gggttgagcg ttggaatgag
     1321 cccttggaat gagcgttggg acaggcgtac gtactacaat gtgatcagcc tgattgcagg
     1381 acaacgtgaa accgcaggct gacacataga taatactgaa ccaaaaggca agcaccagga
     1441 agaacaacac caggcttccg tagatcattt gacgaacatc attcatagtt cggctgctcc
     1501 ctattcctgt gcttttagtg tgcggacgaa gttgaccaca tcccagcgct ccggcacggt
     1561 caggttttca ttgagtggag gcatacgtcc gtcaataccg tttgagatcg tcaggaagaa
     1621 tgaaccgtcg ctcttggctt gcaccacatc cgaagttaga ttcgcaggtt taaccgccag
     1681 aaacggagct acaggtccgt tcccgccgcc ggttggtcca tgacacatac gacaattgat
     1741 ggcataaagc tctgcgccgc gcgccagaga ggcgtcatcc acctcgaagg gattttcagg
     1801 ggcgcccata ccggggatag agattgcccc ttcgatcggt atcgactggg cagggggcgg
     1861 aagcggatct tccatggggc gataggccgg ttggatttcc ataaagctga tccactcgat
     1921 tttgatgatg tcataggaaa ataataggac caccccaccg agaaacccaa gtgtcaagaa
     1981 gaccccaaag agctgtctga ataatctcat agctgttgca cctccacagg tctcacggac
     2041 tcagcaccga aaccactcag cacatccaca tatttttcct gttcacctgc cggatacttg
     2101 aagaagaccc cgatcttgcc atcactgatt tcagggacat attccatggg ccggtaattc
     2161 gggaaataac tatccaggaa cacgcccagg aaggtagaaa gcagggcgaa aagcatcgtc
     2221 atttcgaata ttagaatcat gccgggtggc acgggtgtga tgtactggcc accgaccggc
     2281 acgttataca ggagcggcgt tccataattc agaaagaacc caatacaaaa accaaccacc
     2341 gcgccgccca atgccagccg cgagacattc gtccagggat ggggccgccc caggattttg
     2401 tgtgcaatag gcacaccaga aatcacattg atgttttcat ggggaatacc catctcatgc
     2461 aacttctcaa ttgcatctgc tgccgggtca attttatctt cgtcaaagag agccaacagg
     2521 gttgtttcag tcatgttgtg ttccatttat tcgaattcac tgatggtggg aagattggct
     2581 ttacctactt tgcgcatcga gtgcgtcatc tgcccttcct gcacttccca aacggggatg
     2641 agcgggatga gtcgggaggc tgccaggtag aacaacagga agagtgaaaa agttccaatc
     2701 gtcaggaaga tttcgatgcg cggttcatac aatttccacg tgaagggcat acggttgatc
     2761 gtcaacatca ccggcacgat gagatagcgt tcccaataca taccgatgtt gatcaggatg
     2821 ccgatcagcg caagtaacca gggtgtttgg cgtatccttt tattccacaa cgtaagagcc
     2881 gggatcgcca ggttgaacaa aagcaattgg tagaacatcc aggacatcgg tccctcttca
     2941 agccaatgca gaagttcatc cgtccacttg tctccgccat accactgtac gatgtagtcg
     3001 ttgaagaaga agtacgtata cgcaaaggta acgatcaaca tcaatttacc gagcgcgtcg
     3061 aaatgctctt tgcggatgaa gtaatccatg tgtttcatcg tgtagcgcat gatgaacaac
     3121 accacggcca ccgcgcccat gcctgaatgc aaggcaccca gcacgaagta aggaccaaac
     3181 accgtggacg accagcccgg gcgcatcgcc atagcaaagt cccacgacac gattgtgtga
     3241 accgagaaca taaccgggat gatcgcaaag gcaaagatgt tcatggcggt cgtcaggtga
     3301 cgccattcgc cttcggtgcc gcgaaaaccc aatgccaaaa ccctgtagaa gattttgcgc
     3361 cagcctgtcg agcggtcgcg cgccatcgcc agatctggaa tgagaggcag gaacaagtac
     3421 atcgtactac ccagaacgta tgtggtaatc gcaagcaagt cccacatcaa cggcgagtgg
     3481 aagttcggcc ataactgtcg ctcattgggg atcgggaaca accagtatgc caaccacaca
     3541 cgacctaaat gcatgaagat gctggccccg gcctggatca gaccaaaggt cgtcatcaat
     3601 tctgctgcgc gcgtgaatgg acgtcgaaac tcagctttca tcacacgcag gatcgcggaa
     3661 ataaatgtac ctgcgtggct gataccgatc caaaacaccg tgttgacgag aaagatgccc
     3721 caatacgaag gacgcatgac acccgccaca cccagaccat tcgcaatcat gtagccccat
     3781 gcatacacaa agcatgtagc aacgaggacc gccaaaatag caaacaccac ccagaacttc
     3841 catgaagtgg tgagcatgga ttccatcacc atgtcattca tctccccgcg cggcttgggg
     3901 tcatacatca agtattcgcg cgggtcatgt tcctcttcat gatgggtctc gtgccctcgt
     3961 tcagcaccgg aaaaatattt ggaggtaacg gccatagcct aacctttctc aagataagta
     4021 atacgcggca gggtattaag ttcttccagg tgcttataac cgcggttctg atgagccaat
     4081 tggctcacta aactctcggg gtgatcgata cgaccgaacg taatggcatt agccgggcaa
     4141 gcctgagcgc aggcggttgt gaattctccg tcctccactt cgcggccttc cgattttgct
     4201 gtgtcctgtg ccttatgaat gcgctggata caaaacgtgc atttctccat gacgccgcgc
     4261 tggcgcacgg tgacatcggg attgaactga ttatgaagtg ggaaaacaag ttgtccgtcc
     4321 acattgttcg catggaaggg ctgtttgtga tagtcacgcc agttgaagga acggacctga
     4381 taggggcagt tgtttgcgca ataacgtgtg cctacacagc ggttgtaaac ttgcaggttg
     4441 agctcttctg cgttgctgtg aggtgtggca aagaccgggc acaccggttc acagggggcg
     4501 tgtccacatt gctggcaaag catcggctga aagccgccgg ccactttaat ttccggatat
     4561 tcgccatccc aaaatcgttc gatgcggatc cagtgctgag tacgtccgta gcctgcatct
     4621 tcctctccga cgaatgagag attgttttcc atggcgcagg ccgcaacgca agcctgacaa
     4681 cctgtgcaaa gatcctggtc aatgaccatt ccccatttgc cgggagcctc ttctgcgatg
     4741 gcgcctttcg cgcccatttt gtttaggtct atgctcaaac tcatgatttc tcctctaagg
     4801 atgttcttcg cccatacctt cacgatacac gccgattcgg ctctcgaggc gtgaaagaat
     4861 ctgtttccga ccggtcttct caatatgaac cttcatgcct gcgaaggtta gatctccggc
     4921 ttcattgaaa tgtgcaccaa gcaaagcaac gggatttgca ccgcgcccct gagcatagcg
     4981 accataagca gtatgaccct gcccgaatgg catcgcgatc acttccggat gaattgccgg
     5041 atagagatag accggcgctt ctacttctcc cgcgtcactg atgattttca cgacatcatc
     5101 gttttcaaga ccaagctcat gtgcagtctc agggttgatc tcaacccagg tgttccacat
     5161 aacggtggtc gttggatcag gtaattcctg aagccagggt ttgtttgcac cggcttcgcc
     5221 caacgtcggg gacaggaaag gcaggaagaa gaattcacct tcacccacaa actctgcgtg
     5281 ttcaaccgtc aggtttgatg aaccagccgc ggtaggcgtc agcagctcgg ctgcgttatt
     5341 ccaccaacct ccatgctgct ggaaataggc agtgaaggta ttgatctcgg gtgctgagaa
     5401 agaaccatct gcctgaccga caaggctggc aaacttgctt tgaagatatt ccatttcatc
     5461 tttgaagggc aaggcctgcg aggcagccag cagcacatct gcagtcgcac gcgtatcatg
     5521 catcggcgaa acgactggtt gcgctcctga gagcacagat tgtaagctac cggttgccac
     5581 acgctgataa ccccacgatt cgagaccatg atgatcgggg aatacatagt cagcggcaag
     5641 cgcggtttca tcggggaaag tggcaaacga aattacggtt tcaacgttgg ccaatgcttc
     5701 ggcaaatccc aacgaggttg gcaattcata aaccggattt accccatgaa tgaacaaagt
     5761 ctgaatttca ccgctgttca accgattaat aaattcggtc aattcctgtg cgctcgccgg
     5821 gcgatgatat tcatcctgat tcggcgaaag cggagagaaa gcaacaccac cgggtttgcc
     5881 caggttatcc acgacggcat tcaaagacag gatggcttcc gccgtctgta gtccgttgct
     5941 caaacccagg gcacttcctc cgggaattgc gatcgggctg gtggattctg caacaaggtg
     6001 cgctatgtgc tcaagtgttt cgagattcag acccgtctca gccagaactg cattcaggtc
     6061 aacggcttca aatgccgctg gcgccgcaac accgcgaatt tctgccacca gccgaccaat
     6121 ggcagccgca accagtcctt cggtccccgg atgaattggg atccactcat ccgctttggc
     6181 agccgtttgc gacatacggg actcaaactg gataaagtaa ccgcgcaccc tggggtttcc
     6241 acgacgcatc tgcgaataac cacggttata agccacaggc gaaagccagg tctcaagaaa
     6301 atttgcaccg aacgagagga cgagatctga agtgcccata tcaaagaatg gcattgcggc
     6361 ctgtccgaaa agattctcag atgctttact gagggttgcg cgtgtttcaa acatgctgga
     6421 tacactgaag cgaacgggcg cagacgcacc cgtggaaccg gcaatttcag ttaccagatc
     6481 aaaaagatga tcgggtgcgg taccgagcag gaatgccacg ctaccagaat tttgaagcgc
     6541 acctgccaca acttgcaccg cttcatccca gttcatattc ggctctgcat cctggaagtt
     6601 agcgtcgtac agtccttcgc cacgcgcatg tcgtaaggga cctcgaacac gattcggatt
     6661 atataagccc tgtaacgtga tttgcccacg cggacaagtc ttaccgagat ttaccgggtt
     6721 agccgggtta ccttccactt tgatcgcccg cccctgcatc gtgcggacga tgagtccgca
     6781 gcc
//
LOCUS       DASTJP010000747         5967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1227932, whole genome shotgun sequence.
ACCESSION   DASTJP010000747 DASTJP010000000
VERSION     DASTJP010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227932"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2079
                     /locus_tag="VFQ23_21750"
     CDS             <1..2079
                     /locus_tag="VFQ23_21750"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9909287.1"
                     /translation="GEFRLRPLLKLGLSLPAWLLKGIGPLTRKWLFAPPHALKQFYAQ
                     NMSRWNGWEKFAALEVPTLVIRGNRDLVFERTRFEKVTTSIPGAEESDIGVSGHMVML
                     ERRDAVNRAIERFMSGTDGEQHSWRESSDTTPKKKSERDPLRKKRPWLENYERGVPYT
                     VDVPRIALHHLLRSAVRRFPTNPAIFFEGTRLSYRRLNREVNRFANALLALGIGKGAR
                     VVLLMPNVPQMVIGFYGTMKAGATAVFIPPVIEPEEVVRQVKDSDASVLVTLSMWAGL
                     AKQIQEGSSLPHIILTDPADYLSLPKFLISNWRNRDFKLANSVRWNDFLLGQSNKSPT
                     VEVLPEDLAVIQYTGGTTAQSKGVMLSHRNLVANALQTRHWLPDANEGKERFLCVVPL
                     FHSYGLTTALNTPVSLGAAMILKPQFQILDVLKTIQAYEPTIFPGVPSMYVAINNFRG
                     VRKYGISSIKACISGSAPLPVEVQEAFEKLTKGRLVEGYGLTEASPVTHANPLSDKRK
                     IGTIGVPLPSTETKIIELGQRSKEVKQGQIGELAVRGPQVMLGYWKDPKATQDVITED
                     GWLLTGDVAQMDEDGYTRIVARKADMWYPTQLKKTPAFPRDVEEVIHEIPQVKEVAVV
                     PIAGKPFAFIIAGKEAPTPAAVIAYCKRRLPSHLVPRFVIFMDDFPRTFIGKVLRREL
                     ARRYEQHISE"
     gene            2057..2890
                     /locus_tag="VFQ23_21755"
     CDS             2057..2890
                     /locus_tag="VFQ23_21755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9909288.1"
                     /translation="MNSISPNEINIPHEANDVRQGEGTPVVLIHGIAASLHDWDDLIP
                     ALIENGHAAYALDLLGHGDSPKPDSNAYQLDWLYEHFSTWIRSLHLTEPAIVIGHSLG
                     GHLALEYARRDSAWTRGLVLVNPFYSRSQLNRLIRRPYSQRNLRGLIAGKTPEWLYRA
                     IVDIASVAMRPDNNTLHSLPAKVRAQTALDYTRTAPGIYHVPNTIGDLTEHASSITIP
                     ALVVWGERDQTLAPSSFPKLVEAMPRAKGRAFRAGHVPHQSHAEEFNQVVMEFIRGLS
                     A"
     gene            complement(2913..3641)
                     /locus_tag="VFQ23_21760"
     CDS             complement(2913..3641)
                     /locus_tag="VFQ23_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9909289.1"
                     /translation="MDTSTRAPRYDLFKLVVAILLLLLFICLLLWIPANAPLPSTPTS
                     TPFLLTETPTADETSTSAPPTDLPPPSPSSTSGAATSTPPADPSATQTATVSASRTAT
                     PTPFSEPTSTFTAEPSPTQIVTLTSTGTPASELTATEATETEEPPSGAYTCESALSRS
                     RLQVGGDATILRRLNFRSSPGIQNNWLRTNLSGTKVEVLDGPVCLPFSRGAYVWWQIR
                     LPDGQVGWSAEISLLGRFYFMEPN"
     gene            complement(3649..4086)
                     /locus_tag="VFQ23_21765"
     CDS             complement(3649..4086)
                     /locus_tag="VFQ23_21765"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9909290.1"
                     /translation="MGVIASIVLGLLLGWLLEWVIDWFYWRGRMRSIAEENARLSKDN
                     EDLKERLATLETKVSRKSQLSKTRPTGDRSNKDNLQAIKGIGPVIAKRLNEAGIYTFE
                     ELSRLTPEELQEVMGPLAKRFFPKQESILTQAKDFAEQKAQKS"
     gene            complement(4403..4825)
                     /locus_tag="VFQ23_21770"
     CDS             complement(4403..4825)
                     /locus_tag="VFQ23_21770"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9909291.1"
                     /translation="MKVIKLFILGLILGLFYKWLTSRVYTDIEISTITTEDPLDMDSL
                     KTEYIKSLDTKEKLTAEVKPVVPKTKKEILEVLKGIGPASVKRLNNAGVHTFDELAQL
                     EPEDLRAILGNVLKRAGITESQVISEARKFAQQKIEQE"
     gene            5059..5508
                     /gene="nrdR"
                     /locus_tag="VFQ23_21775"
     CDS             5059..5508
                     /gene="nrdR"
                     /locus_tag="VFQ23_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET9909292.1"
                     /translation="MRCPYCQHHDSKVLDTSHDSHGGIRRRRECFKCGQRFSTYERPI
                     LATPLIFKQDGTREEFDREKLARGIRISCAKRPVSAADIERLIGQVESQLQKLGKSEV
                     SSRVVGDLVMNGLKEMDQIAYIRYGIVYLGLDDLQSLRNEIDRLLEG"
     gene            5682..>5967
                     /locus_tag="VFQ23_21780"
     CDS             5682..>5967
                     /locus_tag="VFQ23_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HET9909293.1"
                     /translation="MATKAVPQTTRVKNGLLPTPPMPDGLPQANLTDNARQVLMKRYV
                     RRGDDGKPAESVEEMFWRVAYHVAKVEEQWDGDIQKRTIEYYHLLSSKKFF"
BASE COUNT         1446 a         1445 c         1581 g         1495 t
ORIGIN      
        1 ggcgagttca gacttcgtcc attgctcaaa cttgggctga gcctgccagc ctggctgttg
       61 aaaggcatcg gcccattaac gcgcaaatgg ttatttgctc cgccacatgc gctcaaacaa
      121 ttttatgcac agaatatgtc gcggtggaac ggatgggaga aatttgccgc acttgaagtg
      181 ccaacccttg tcattcgcgg taatcgggat ttggtcttcg agcgaacgcg gtttgagaag
      241 gtgaccacct ccattcccgg tgccgaagag tcagatatag gcgtatccgg tcacatggtc
      301 atgctggagc gaagggacgc tgtgaaccgc gccattgagc ggttcatgtc aggaactgat
      361 ggtgagcagc actcctggcg cgaatcatca gatacgactc ccaaaaagaa aagcgaacgc
      421 gatcctttgc gtaaaaagag accctggctg gaaaattacg aacgcggtgt gccttacacg
      481 gtcgatgtac cccgcatcgc gctgcatcat ctgttgcgat ctgctgtgcg acggttcccg
      541 accaaccccg caattttctt tgagggaacg cgtctttcct atcgacgatt gaatcgagaa
      601 gtcaatcgat ttgccaatgc ccttctcgcc ctgggaatcg gaaagggcgc tcgggtggtt
      661 ttattgatgc caaacgttcc gcaaatggtg atcggttttt atggaacgat gaaagcgggt
      721 gcgacggcag tattcattcc accggtgatc gaaccggagg aagtagtccg ccaggtaaaa
      781 gattcggatg caagtgtgct cgttacatta tcaatgtggg cggggctggc taagcaaatt
      841 caggagggca gtagccttcc gcatatcatc ctgactgacc cggcggatta tctttcgctg
      901 ccaaaattct taatctcaaa ttggcgtaat cgtgatttca agctggccaa tagcgttcga
      961 tggaacgatt ttcttttggg gcaatcgaac aaatcaccaa cggtagaagt attgcccgaa
     1021 gacctggctg tcattcaata tactggcgga acgaccgcac aatccaaagg tgtgatgctt
     1081 tcccatcgca atctggtggc aaatgccttg cagacccgtc actggctgcc cgatgccaat
     1141 gaaggcaaag aaaggttttt atgtgtagtc ccgctctttc acagttatgg cttgacgacg
     1201 gcgcttaata caccggtctc gcttggcgcg gcaatgattc tgaagccgca gttccaaata
     1261 ttggatgtgc tcaaaactat tcaggcgtat gaaccgacca tttttcccgg cgtgcccagc
     1321 atgtacgtcg ccatcaataa tttccgcggc gtgcgcaaat atgggatcag ttcgatcaag
     1381 gcttgtatca gcggatctgc accgttgccg gtcgaagtgc aggaagcctt cgagaagcta
     1441 acaaaaggca ggcttgtgga aggctatgga ttgaccgaag cctcgccggt cacgcatgct
     1501 aatccgctca gcgataaacg caaaataggg acgatcggtg tgccgctccc ttcgacagaa
     1561 acgaaaatca tcgagttagg tcagcgtagt aaagaagtga aacagggcca gatcggtgag
     1621 cttgctgtgc gcggtccgca ggtgatgttg ggatattgga aagacccaaa ggcgacccag
     1681 gacgtgatca ccgaagatgg ctggctcctg acgggtgatg tggcgcagat ggatgaagat
     1741 ggatataccc gcattgtggc gcgcaaagca gatatgtggt acccaaccca actgaaaaag
     1801 acacctgcct tcccgcgtga tgtggaagaa gtgatccatg aaattccaca ggtgaaggaa
     1861 gtggcggtcg tgcccattgc aggtaaacca ttcgccttca tcatcgcagg gaaagaggcg
     1921 ccgacgccgg cggcggtcat cgcctattgc aaacggcgtt tgccgtcaca tctcgtgccg
     1981 cgcttcgtta tctttatgga cgatttcccg aggactttta ttggcaaggt attacgacgt
     2041 gagttagcta gaaggtatga acagcatatc tccgaatgaa ataaacattc cccacgaggc
     2101 aaatgatgtt cggcagggcg agggcacacc ggttgtgctc attcacggca ttgctgcctc
     2161 tctgcacgat tgggatgatc tgattccggc gctgatcgag aacgggcatg ccgcatacgc
     2221 ccttgacctg cttggtcatg gcgatagccc aaagccggat tcaaatgcct atcaattgga
     2281 ttggttgtat gagcattttt caacgtggat tcgatccctt catctgacag aacccgccat
     2341 tgtcattggg cattcccttg gtggacatct cgccctggaa tacgcacgcc gcgattctgc
     2401 ctggacgcgc ggactggtgc tggtcaatcc attttattca cgttctcagt tgaaccgcct
     2461 gattcgacgg ccatatagcc aacgaaattt gagaggctta atcgcgggga agactcccga
     2521 gtggttgtat cgcgccattg tggatatcgc cagtgttgcg atgagacccg ataacaacac
     2581 cttacactcc ttgccagcca aagttcgggc gcagactgcg ctcgattaca cccgcaccgc
     2641 acccggaatc tatcacgtgc ccaacacgat cggggactta accgagcatg cctcttcgat
     2701 cacaattccc gcgctggtcg tttggggaga gcgcgatcag acgttggcac cgtcgtcgtt
     2761 tcctaaatta gtagaggcaa tgccgcgcgc aaaggggaga gcttttcgag ccgggcatgt
     2821 accgcatcaa tcgcatgcgg aggaattcaa tcaggtagtg atggaattta taagagggct
     2881 ttccgcgtag gacaggttgc caatctgtcc tactaatttg gctccataaa ataaaatcgt
     2941 cctaaaagag agatctcagc tgaccagccg acttgcccat cgggcagccg aatctgccac
     3001 catacatacg cgcctctgga gaacggcagg caaactggcc catcaaggac ctccaccttt
     3061 gtaccagata aatttgtccg gagccaattg ttctgaatgc ctggcgaaga ccggaagttc
     3121 aaccgacgga gaatagttgc gtccccgccc acttgtaagc gtgagcgtga taacgctgac
     3181 tcgcatgtat aagcgccgga aggtggttcc tcggtttccg tagcttccgt agcagtcagc
     3241 tcgcttgcgg gagttccagt tgaagtgagc gtaactatct gagtgggcga gggctccgct
     3301 gtgaatgttg aggtaggttc agaaaatgga gtcggcgttg cggtgcggga tgctgacacc
     3361 gttgcagttt gcgtggcaga tgggtcagcg ggaggagtcg atgtggctgc accagatgtg
     3421 gacgaaggtg acggcggtgg aaggtctgtg ggtggggcgc ttgtagaagt ttcgtctgcc
     3481 gtcggagttt cggtcagaag gaagggtgtg ctggttgggg ttgagggcag tggtgcattt
     3541 gctggaatcc ataacagtag gcatataaat aacaacaata gaagaatcgc gacgactaac
     3601 ttgaacaggt catagcgcgg tgcgcgcgtc gatgtatcca tgcgatcctt agctcttctg
     3661 cgctttttgt tctgcgaagt cttttgcctg cgtcaggata ctttcctgct ttgggaagaa
     3721 tcgtttcgcc agggggccca tcacttcctg aagttcttcc ggagttaatc gtgataattc
     3781 ttcaaaggta tagattcccg cttcattcaa tcgttttgcg atgacaggac caatcccctt
     3841 aattgcctgc aaattatctt tgtttgaccg gtcaccggtc ggtctcgttt ttgaaagttg
     3901 agactttctg ctcacttttg tttcgagagt ggcaagtcgt tccttcaaat cctcattgtc
     3961 tttgctcagc ctggcgtttt cctccgcaat cgagcgcatg cgcccgcgcc agtaaaacca
     4021 gtcaatgacc cactcaagca gccagcctag cagcaaacca aggacaattg atgcgatgac
     4081 acccataatg cagccctttc tgttgtgcgg taacgtgaga gaattctatt gtatcagaag
     4141 ggtgcgggta tacaatatcg cgttgatcgg atgagagtgg ctaagagagt gagtgaaact
     4201 ctacttagcc acagagagca ctgagcccat tgaccccgca cctgcgttcg gggcaggtgg
     4261 aaaaaaactc aaaaactctg tgggctctgt ggcttacaca ccaaattcgc cactcagtgc
     4321 gagttccttt ggttgatgca agatttcaat ataacgggat gaagtgtcac tgaggaaaga
     4381 caagaaaaga aataccggcc gcttattcct gctctatctt ttgttgtgca aatttccttg
     4441 cctctgaaat cacctggctc tctgtgatcc ccgcgcgctt taatacgttt ccaagaatgg
     4501 cacgcaagtc ttccggttca agttgcgcaa gttcatcaaa cgtgtgaact ccagcattgt
     4561 tcaatcgttt cacacttgcc ggtccgatcc ctttgagcac ttccagaatt tctttcttgg
     4621 ttttcggtac aacaggcttg acctctgccg tgagtttttc ttttgtatct agcgatttaa
     4681 tgtattctgt tttgagtgaa tccatgtcga gtggatcttc cgttgttatc gtgctgatct
     4741 caatgtctgt atagactcgg cttgtaagcc atttgtaaaa caaaccgaga atgagaccaa
     4801 gaataaatag tttgatcact ttcattgctg agtcctttca gtatgatttc tatgtatctt
     4861 aacgtagtca tgtgggatta caatacacta catcattttt tcacgaaaaa ttttaaggtt
     4921 catcccccgc ctgtcctctt gaattgaggt taggtctatg ctaaaatggt cttcaagata
     4981 accatatgta cggggatgca gcgctcctac gcccacagat ggcgctaaca aatgttctaa
     5041 tattggagtg atctatctat gcggtgtcca tactgccagc atcacgattc taaagtgctc
     5101 gatacatctc atgatagcca tggtggaatt cgtcgacgcc gcgaatgctt caaatgcggg
     5161 cagcgtttca gcacgtatga acgaccaatc ctggcaacgc cgctcatttt caagcaggat
     5221 ggaacacgag aagagttcga ccgggagaag ctggcacgcg gcatacggat ctcctgtgca
     5281 aagcgacccg tctctgcagc cgatattgag cgtttgatcg gacaagtcga atcacaacta
     5341 cagaaattag ggaaatcaga agtgtcttcg agagtggtcg gtgacctggt gatgaatggt
     5401 ttgaaggaaa tggatcagat tgcatatatc cgttacggca tcgtctattt gggtttggac
     5461 gatcttcaat ccctgcgcaa cgaaatcgat cgccttctcg aaggctaatt gataaatgac
     5521 agtgaccctc cgcccggggc ggagtgtgct cctgtcattt tttgttgctc gttactttcg
     5581 cgctggtcga gaccagcagg ttaagtcaaa gtgtgtgttt cttagttagt cgctcctcgt
     5641 ctgcaactca ggacgaataa aattaatttg gagagtgtac tatggcaacg aaagcagttc
     5701 cccaaacgac tagagtaaag aacggattac ttccaacgcc tccgatgcca gatggattgc
     5761 ctcaggcgaa tctcaccgac aacgcgcgtc aggtgttgat gaaacgttac gtccgccgcg
     5821 gggatgatgg caagcccgcc gaatcagtgg aggaaatgtt ctggcgcgtg gcgtatcatg
     5881 tggcaaaggt cgaagagcag tgggacggtg atattcaaaa acgcacgatc gagtactacc
     5941 atttattatc cagcaagaaa ttttttc
//
LOCUS       DASTJP010000748         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_115573, whole genome shotgun sequence.
ACCESSION   DASTJP010000748 DASTJP010000000
VERSION     DASTJP010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115573"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFQ23_21785"
     CDS             complement(<1..453)
                     /locus_tag="VFQ23_21785"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HET9909294.1"
                     /translation="MATVPGIDVSYWNAGIDWPKVRATGQRYVFTKATEGSSYSDPTF
                     DDNWRGAKRAGLLRGAYHFFRCNVDPKKQAARFIDYVQSMNDNGELPPVLDLETNDGQ
                     TKDKIIERAKIWLDTVEAAFGKKPIIYSGQYFLQDYFSELGGGPPAWAK"
     gene            595..774
                     /gene="ssrS"
                     /locus_tag="VFQ23_21790"
     ncRNA           595..774
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFQ23_21790"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(827..2260)
                     /locus_tag="VFQ23_21795"
     CDS             complement(827..2260)
                     /locus_tag="VFQ23_21795"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HET9909295.1"
                     /translation="MRSEHSIPEYVDVVKRGITAKFNDEKPKKVIIVGAGLAGLSAGY
                     ELLRAGHEPLILEAQQRVGGRVYTLREPFARGLYGEAGAMRIPRSHALTMAYIDRFGL
                     QTSDFSMGNPEAYVHVGGVKRRMAEVLAAPELMGFELAEIEKGKISGLYWEETIRPLV
                     EKVQKEGDVGWEQIYAEFDQYSVREFLELKGWSEGMIEMFGLLNNQEAMMNSSFLELF
                     REDGGNYYTNMCQVVGGSDNLPHAFLPELGSRIRYGAKMIAIDQTPDEVIIHYQTRSG
                     RFAVHGDYAILTVPFPVLRHVEVVKPFSRAKQRAIRQLHYDASAKIFFQTRRRFWETD
                     EGIFGGGTVTDLAIRNLYYTDYGKETGRGILLVSYTWSEDAQRWGSLLPHERIEQALE
                     NVAIIHPQIKDEFETGASHMWHDDPFAGGAFALFDPGQQTLLHDDIVKPEGRIHFAGE
                     HASLYHAWIQGALESGLRAAIAIHQSP"
     gene            2678..3544
                     /locus_tag="VFQ23_21800"
     CDS             2678..3544
                     /locus_tag="VFQ23_21800"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9909296.1"
                     /translation="MNTTIDLKQSSEDYLRIEQAISYIENHYKNQPSLEEVAANIGLS
                     EYHFQRLFTRWAGVSPKRFLQFLTKEGAKELLNRSENLLDTTHQVGLSSLGRLHDLFV
                     TAEAVTPGEYKSHGEGVTIRYGIHLTPFGKCLIGLTERGICHLGFVQTSEGDAIDAMF
                     AEWKEAKMIEDYRATAALVEPIFNLRYNTRIKPLTLHLRGTNFQLKVWEALLTIPAGS
                     VTTYERIASTIGKPGATRAVGTAIGHNPIAVLIPCHRVIRKIGDFGNYRYGEVRKKAL
                     LAREFVNTSMVE"
BASE COUNT          922 a          914 c          888 g          931 t
ORIGIN      
        1 ctttgcccag gcgggaggtc caccgcccag ctctgaaaaa taatcctgga ggaaatattg
       61 acctgaataa atgatgggtt ttttgccaaa tgctgcttca acggtatcca accaaatttt
      121 tgcccgctcg atgattttat ctttggtttg accgtcgttt gtttcaaggt cgaggacggg
      181 tggtaattct ccgttatcgt tcatggattg cacgtaatca atgaacctcg cagcttgttt
      241 tttaggatcc acgttgcatc ggaagaaatg ataggcaccg cgtaacagtc ctgctcgctt
      301 ggcgccacgc cagttatcat cgaaggtggg atcagaatat gagcttcctt ctgtcgcttt
      361 tgtaaacacg taacgttgac cggtcgctcg aactttgggc caatctatcc cagcattcca
      421 ataggaaacg tcaataccag gaacggttgc catttttgat ctccttcaat ttgattggat
      481 cgggacacaa tgcagcataa ggttagcaca attcggtggg gaaatcaaca acagagaata
      541 attagaaatt aatttttatc tacccttgct tcactgttta tggtataaaa acgcacatcc
      601 ctgctgtgca agtgatgttg cacttcccgt tttgagccaa cacttattaa aagggtctcc
      661 aactccatat agaaataacg cgataggcct gagccaaggc ggcgtttctg ggtagggacc
      721 cacctgcgaa agcaggttca aaactcggca gcacacggca ttggcgggga attgaaatca
      781 aaagacctga gaggtttcgc aaagcgaacc tctcaggtct tattgtttac ggcgattgat
      841 gaatcgcaat ggcggcgcgg agtcctgatt caagcgcgcc ttgaatccag gcgtgatata
      901 gcgaagcatg ctccccagca aaatggattc taccttctgg cttgacaata tcatcatgca
      961 gtaaagtttg ttgaccggga tcaaacaatg caaacgctcc acctgcaaat ggatcgtcat
     1021 gccacatgtg tgaggcgccc gtttcaaatt catctttgat ttgcgggtgg attattgcga
     1081 cattttctaa tgcctgctcg atgcgttcat gtggcaataa agaaccccat cgctgagcat
     1141 cttcagacca tgtatagctg acaagcaata ttccacgacc tgtttctttt ccgtaatcgg
     1201 tgtagtacag gttacgaatt gccagatcgg tcacggtacc cccgccgaag ataccttcgt
     1261 cagtttccca gaagcggcga cgggtttgaa agaatatctt tgctgaggcg tcgtagtgta
     1321 attggcggat ggcacgctgc ttggcacgcg aaaaaggttt caccacttct acgtgccgca
     1381 agacaggaaa cggcactgtc aggatggcgt aatctccatg aacagcaaag cgacctgagc
     1441 gagtctgata gtggataatt acttcatccg gagtctgatc gatggcgatc atcttcgcgc
     1501 cgtaacggat gcgtgaacct aattcgggga gaaatgcatg tgggagattg tctgacccgc
     1561 cgacgacctg acacatgttt gtgtagtagt taccgccgtc ttcgcgaaac aattcaagaa
     1621 acgatgaatt catcattgcc tcctgattgt tcagcaggcc gaacatttca atcatgcctt
     1681 ccgaccagcc tttgagctcg agaaactcgc ggactgaata ctggtcgaat tcggcataaa
     1741 tctgctccca gcccacatcg ccttcttttt gcaccttttc gaccagtggt cgaatcgtct
     1801 cttcccagta caaaccggaa atctttccct tctcgatttc agccagttcg aaacccatta
     1861 gttcaggtgc cgccaacacc tccgccatgc gtcgcttgac tccgccaaca tggacatagg
     1921 cttcagggtt acccatcgaa aaatcagacg tctgtaatcc aaacctgtcg atgtacgcca
     1981 tcgtcaaggc atgcgagcgt gggattcgca tcgcgcccgc ttccccatag aggccgcgcg
     2041 cgaacggttc acgcagcgta taaactcgac ctcccacgcg ttgctgcgcc tctaagatca
     2101 agggttcatg gccggctcgc agcagttcgt acccggcgga aagtcccgcg agacctgctc
     2161 caacaataat gactttcttg ggtttttcgt cattgaattt tgctgttatc ccccgtttga
     2221 cgacgtccac atactctgga atggaatgtt cagatcgcat gtatgcctcc gaaaatcaag
     2281 acgcatcccg ccgaaattcg atacaagttt cacgaaccga tttttgactt gacgaagcaa
     2341 ctctgaaccg tgtagaatca ccgccgttaa caatcgaata tcggagaact agcggctggg
     2401 tccttttttt ggtttcgaca catgcatgat ttgccatgaa atgctcacgc atttcaaaga
     2461 tcacatgtct gtgctagtga tacaggtgta tctctggtac cctttgtaag ggctgtctcc
     2521 tttaatgcta ggtcatttaa aaggcatgcc cgggcatgcc ttttttgttt tgacctcacc
     2581 ccccaacccc tttcctaaag gagacgggag taaatgagca agaatcgggt cgtcatatgg
     2641 aagtgatgtt catagaatag aatcaaaagg agcaactatg aacacgacca ttgatctcaa
     2701 acaaagttct gaagattacc tgagaatcga gcaggccatc tcgtacatcg aaaatcatta
     2761 taagaatcag ccaagccttg aagaggttgc cgcgaatatt gggttgagtg aatatcactt
     2821 tcagcgatta ttcactcgct gggcgggagt cagccccaag cgcttcctgc aattcctgac
     2881 gaaggaaggc gcgaaagaac tgctgaaccg ttctgagaat ctgttggata cgactcatca
     2941 ggtcggtctt tcaagcctgg ggcgtcttca tgatctgttt gtcaccgcag aggctgtgac
     3001 gccaggggag tacaaatcgc atggcgaagg agtgacgatc cgctacggga ttcacctgac
     3061 gccttttggc aaatgcttga tcgggttgac ggagcgcggt atctgccatc ttggctttgt
     3121 gcagaccagt gaaggtgacg cgattgatgc gatgtttgca gaatggaaag aagcaaagat
     3181 gatcgaagat tatcgagcca cagccgcttt ggtagagccg atttttaact tgcgatataa
     3241 cacgcgcatc aaaccgttaa cgctccacct gcgcggtaca aatttccagc tgaaggtatg
     3301 ggaagcactg ctgaccattc ctgccggctc ggtcacgacc tacgaacgga tcgcctcaac
     3361 aatcggcaag cctggcgcga cgcgtgccgt cggtactgct attggtcaca atcccatcgc
     3421 agtgctgatc ccctgtcacc gcgtcatacg taagattggt gacttcggaa actaccgtta
     3481 tggagaagtg cggaagaaag cgctgctggc aagagaattc gtaaatacgt cgatggttga
     3541 gtagttctaa gccgcttcat ggcggaaata gatcgatacc acctgttttg tgatttactc
     3601 ttgcggcatg ttggtgtcga tacgggctgc gccccattca gccctactca accaa
//
LOCUS       DASTJP010000749        10715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_911618, whole genome shotgun sequence.
ACCESSION   DASTJP010000749 DASTJP010000000
VERSION     DASTJP010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911618"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFQ23_21805"
     CDS             complement(<1..915)
                     /locus_tag="VFQ23_21805"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9909297.1"
                     /translation="MNYKLWIDGKWEDTQGGGVMSIEDPTTGKAIAEVIDASRQDVDR
                     AVQAARRAFYDGRWSKKTPSERSKIIWKLADLIESNAEALAKVESENTGKPYAFVSLG
                     GDIPFAVDNLRFFASAARDTHGSHAGEFMTGYTSMYRREPVGVVGQIAPWNYPVMMAA
                     WKIGPALAAGCTIVLKPAPGTPLTTLMLAEIIQEAGIPDGVVNIISGGNDAGQAIVEH
                     PDVRMVSLTGSTGTGKKIMKTAAETLKRVHLELGGKAPFIVFDDADPELVAAKASMAA
                     TLNTGQDCTAATRLYVEKGKAQEMQDAVV"
     gene            complement(937..2361)
                     /locus_tag="VFQ23_21810"
     CDS             complement(937..2361)
                     /locus_tag="VFQ23_21810"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9909298.1"
                     /translation="MEYKLLIDGQWVNSGPTMEVKNKYDGKVIGTVPTASKENVDAAI
                     DAAERAEDSMADMPAHKRSEILLRTAALLRERSEDLAKTIAAEAGKALKFARAEVDRA
                     VSTFTIASEETKRIHGETIPLDAVPAGEGYVGFWTRRPVGVIAAISPFNFPLNLVAHK
                     VAPALAAGNTIVLKPASTTPLAAVKLCQILQEAGLPPGAINLVVGSGGTVGEWLVTDE
                     RVDKITFTGSPDVGRHILSVAGIKKVTLELGNTSPVVVAPDADLDFVAKRAAVGAFYN
                     SGQVCISVQRIYSEKQVYEPFSEKFVKATEAMVVGDPLDERVDVGPMIDVKEVDRVEG
                     WVKEAQTSGAKVLTGGRRDGTVYYPTILADVDDDMKVVAEEVFAPVASVISSDDFESA
                     LKQANDTKFGLQVGVFTNDVNRVFRAVKRLNFGGVIINDTPNFRADHMPYGGNRQSGL
                     GREGVKFAMEDMTNIQLVAIRLNS"
     gene            complement(2519..3958)
                     /locus_tag="VFQ23_21815"
     CDS             complement(2519..3958)
                     /locus_tag="VFQ23_21815"
                     /inference="COORDINATES: protein motif:HMM:NF010000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HET9909299.1"
                     /translation="MDYRLWIDGQWVDTQGGGKMSIENPATGETIAEVQNASRADVDR
                     AVQAARKAFYDGRWSKKTPSERSKAIWKLADLLEANSEEFARVESENTGKPYKFLSLA
                     SDIPFTVDNLRFFATAARDTHGSHAGEFMAGYTSIYRREPVGVIGQITPWNYPLNMAG
                     WKLGPALAAGCTIVLKPAPGTPLTTLMLAELIQEAGIPDGVVNIISGGNDAGQALAEH
                     PDVRMICVTGASSTGKKVMKTAAETLKRVHLELGGKAPFIVFDDSDPELVAAKASFAA
                     TANTGQDCTAATRIYVEKGRANDMREAVVEAMKNVKVGLPFEDDVVMGPLISGIQRER
                     VSGFVDRAKSQGAKILVGGGVPKDLQKGYYYEPTVISDVRQDWEIVQSEVFGPVITVQ
                     EFENEAEALHLGNDVLYGLAASVFTKDVGRAMRLTSQLEFGTVWVNDHLPITSETPHG
                     GFKQSGFGKDLSSEAVGDYQITKHVMIAH"
     gene            complement(4069..4269)
                     /locus_tag="VFQ23_21820"
     CDS             complement(4069..4269)
                     /locus_tag="VFQ23_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909300.1"
                     /translation="MLIIEIKENGTLVGELSAPEKQFKTGSRGFFGMGKIQIGEKRYQ
                     VQVQMVEIGSKPKAEEKALRDE"
     gene            complement(4271..5497)
                     /locus_tag="VFQ23_21825"
     CDS             complement(4271..5497)
                     /locus_tag="VFQ23_21825"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HET9909301.1"
                     /translation="MRVLLVGVGGVGEAIAAIAKPREWMKLMVLADYNIQRAEEVQKK
                     LGDADAKRFPIEFIDASKQENIEALAKKYDVDLIMNAVDPIFNKQIFDAAYNIGANYM
                     DMAMTLSEPHPTDPFHMTNVKLGDYQFDKAEAWKEKDLLALVGFGVEPGMADVFARYA
                     QDHLFDEIEEIGIRDGANLTIDGYDFAPSFSIWTTIEECLNPPVIFEEGKGWFTTEPF
                     SEHETFEFPEGIGPLDVVNVEHEEVLLVPRWLKAKRVTFKYGLGDEFISVLKTLHKLG
                     LDNKGTIKVKGVEVSPRDVVAASLPDPAHLGEKMHGKTCAGTWVKGSKDGKPKEVYLY
                     QVADNQACMEQWGCQAVVAQTAFTPVIAMDLIEHGQWKGSGVLGPEAFDSRPFLQKMA
                     DYGFPYGIKEMTKEGA"
     gene            complement(5620..6750)
                     /locus_tag="VFQ23_21830"
     CDS             complement(5620..6750)
                     /locus_tag="VFQ23_21830"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9909302.1"
                     /translation="MKKTLIYMFVLVSLMLAACQPATSEATAPASGGDQTSDRCGDTS
                     QLADEIFLYTWVEYIDPDLKAQFETECGVKVTETNYDSNETLLATLQAGGADYDVIVP
                     SDYMVQLLIEEGMVQELDYNVITNIQNMEPLNVNQYFDPDQKYTVPWFWGTSGFAVDT
                     SVVTDVQPSWNMMFDPNSPYCGQISMLDDERETIGAALMSLGYSINDTDPAHLEEAKN
                     LLIAQSNCVKAYDSQTNDDLIISGETALGHMWTGDAILAGLPDSGGREEIQYVIPQEG
                     CTIWQDNMMIPTGAPNPYTAMIFMNYFQYPEIAAQNAEWVGYATPNKAAKEFIDPEML
                     SNESIYPSAEVTESLQWIEDVGPALELYDRIWTEFKAAVGSS"
     gene            complement(6848..7975)
                     /locus_tag="VFQ23_21835"
     CDS             complement(6848..7975)
                     /locus_tag="VFQ23_21835"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909303.1"
                     /translation="MTADAPAVTRQQARQMRFTTRVGNWLLGTNAALVFGFLYLPVII
                     LILFSFNNTRSVAVFTGFSTEWYVSLAANTELLDAARNSLLVGLITTIFATVIGTLTA
                     LAMDRYRFRLRTAFDANLYLPIVIPEIVMGIALLLFFNQALFPFLQNVFGIRASTGLH
                     TITISHIAFDIPFVYIIVRARLADFDRTLEEAAADLGADEWKTFQRVTLPLLMPGIIG
                     GALMAFTLSLDDYLITVFTKGIREQTMPLYIYSLVRRGVTPEINALSTALLIGSIGLV
                     GLSLTAQNGGAVYTRAMSVGAGLGLGLFGLLSLPALFASFTIGGLIIRLLLIAAFLWV
                     RRAYGPYRDELNEANRSGKILAWIAVSVSAAVAFLSAVLLI"
     gene            complement(7972..8883)
                     /locus_tag="VFQ23_21840"
     CDS             complement(7972..8883)
                     /locus_tag="VFQ23_21840"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909304.1"
                     /translation="MLKFFREKKGFQLFGLIGPGALWILLFFNLPILIVLFISFVERG
                     RAGGIQIPPVYTLENYIQLFNACTSEFSGPQCDPFLYIGIFGHSVRIALVVTFWCIIL
                     GYPLSYFISKQKQVWRDALMILVIIPFWTNFLVRTYALKQVLATEGLLNSFLMNTGLI
                     GQPLDLLFNEFAVIVGLVYGYLPFAILPMYASIEKFDHTLMEAAADLGASPLRAFLKV
                     MLPLTLPGVIAALVLVFVPVVGAFITPDIMGGGKIEMIGTLINRQFGVSRNWPFGASM
                     SLILMVLVLIGVIAYFRSSTEETRRGI"
     gene            complement(8897..9994)
                     /locus_tag="VFQ23_21845"
     CDS             complement(8897..9994)
                     /locus_tag="VFQ23_21845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909305.1"
                     /translation="MAEYAVEMQDVVKKFATPEGGILAAVNHVTMQIKDGEFFSLLGP
                     SGCGKTTSLRMIAGFEMPTEGKILIHGKDVSQVPAFQRPVNTVFQQYALFPHMTVEQN
                     IEFGLEMKGVPRAERSVRVSQALDMVRLPGMEMRKPKQMSGGQQQRIALARALVNKPE
                     VLLLDEPLGALDLKLRKEMQLELKTLQREVGITFIYVTHDQEEALTMSDRIAVMSKGV
                     ALQIGKPDEIYERPATKFVADFIGETNFLNGVVKAQNGNLVEVEIDNTGTVFVESSRK
                     FTNGQQVSVAVRPEKLRLNNERSGDNNLKGRVEEVIYIGTDTHFGVRFAGGQKVRVRE
                     QNIAHTNDEFAKTGDEVMVSFTRISPRILTE"
     gene            complement(10098..>10715)
                     /locus_tag="VFQ23_21850"
     CDS             complement(10098..>10715)
                     /locus_tag="VFQ23_21850"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9909306.1"
                     /translation="FWPRVRDVTKKYGVLLIADEVQTGLGRTGQLWGVNHWNVTPDIL
                     ATAKSLGGGVMPVSTAITTEEIFQPMMYPNPFMHTTTTGGNALACAAAIATINVILRE
                     RLWESAATKGVYLKEKVDDIAQEFPHIYKEVTGKGLLIGQHFHTPEIGYKIAAELFKR
                     GVLVAGTLTSAQTIRIEPPLIISQEEIDEGLNRLTDAVGVVAKTL"
BASE COUNT         2601 a         2946 c         2482 g         2686 t
ORIGIN      
        1 gacaacagca tcctgcatct cctgcgcctt tcccttttca acataaagcc gagtcgcagc
       61 agtacaatcc tgaccggtgt tcagcgtagc cgccatagag gcttttgcag caacgagttc
      121 cggatccgca tcatcgaaca caatgaacgg agctttgcct ccgagttcaa ggtgaacacg
      181 cttgagcgtc tcagcggcag tcttcatgat cttcttacca gttccggtcg agcctgtgag
      241 gctgaccatc cgaacatccg gatgttccac aattgcctga ccggcatcat tgccgccaga
      301 aatgatgttc acaacaccat cagggatacc tgcttcctgg atgatctcag cgagcatcag
      361 ggttgtgaga ggcgtaccgg gcgcaggctt caaaacaatt gtgcagccag cggctagtgc
      421 gggaccgatc ttccacgcag ccatcatcac cggataattc cagggtgcga tctggccgac
      481 tacgccaaca ggctcgcggc gatacatcga ggtataacca gtcatgaact cgccggcatg
      541 cgatccgtgt gtatcgcgcg cggcacttgc gaagaagcgc aggttatcta cagcaaaggg
      601 aatatctcca ccgaggctca caaacgcata tggtttgccc gtattttccg attcaacctt
      661 tgccagcgct tccgcgttgg attcaatcaa gtccgccagt ttccagatga tcttcgaacg
      721 ttcactcggc gtcttcttcg accaacgacc atcatagaag gcgcgccggg cagcttgcac
      781 ggcgcggtct acatcctggc ggctggcatc gattacttca gcgattgcct tccccgtagt
      841 ggggtcttca atcgacatca cacctccgcc ttgcgtatct tcccatttgc catcaatcca
      901 taatttgtaa ttcattgatg ttctcctcga agtagattat gagttcaatc ggatcgctac
      961 cagttgaatg ttcgtcatat cttccattgc aaatttaaca ccttcacgtc ccaaaccgct
     1021 ctggcgattt ccgccgtatg gcatatgatc ggcacggaaa ttgggcgtgt cattgatgat
     1081 gacaccgccg aaattcaagc gcttgaccgc tctgaacaca cggttcacat cattagtaaa
     1141 taccccgacc tgcaagccaa actttgtgtc attcgcctgt ttcaatgcac tttcgaaatc
     1201 atcgcttgaa atcacggagg caaccggggc aaatacttcc tctgccacga ccttcatatc
     1261 atcatccacg tccgcgagga tcgtcggata gtagactgtc ccatcacggc gacccccggt
     1321 caaaactttt gcgccagacg tttgggcttc tttcacccag ccttctacgc gatccacctc
     1381 cttgacatcg atcatcggac cgacgtccac gcgctcatcg agagggtcac ccaccaccat
     1441 cgcttcggta gctttgacaa atttctctga gaaaggttca tacacctgtt tttcactata
     1501 aatgcgttga acggaaatgc agacttgccc ggagttataa aacgcgccaa cggccgcacg
     1561 cttcgcgaca aaatccaagt ctgcatctgg tgcgacaact acgggagaag tgttacccaa
     1621 ttcgagagta actttcttga taccagcgac agaaaggata tgccgcccaa catcaggtga
     1681 accagtaaaa gtgattttgt ccacgcgctc atcggtgacc agccattcgc caaccgttcc
     1741 tcccgaaccc actaccagat tgatcgcccc agggggcaaa ccggcctcct gcaagatctg
     1801 acataatttc accgccgcga gcggagtcgt agatgcaggc ttaagcacta tcgtgtttcc
     1861 tgcagcgaga gccggcgcta ctttatgagc tactaaattg aggggaaagt taaacgggct
     1921 gatcgccgcg atcacaccga cagggcggcg agtccaaaag ccaacgtatc cctcgcctgc
     1981 cggaacggcg tccagaggaa tggtctcacc atggatacgt ttggtttctt ccgatgcaat
     2041 cgtaaaggtg ctgaccgcac gatccacttc ggcgcgcgca aatttgagcg ctttgccggc
     2101 ttccgcggcg atggtctttg ccagatcttc cgagcgttcg cgcagaagcg cagccgttcg
     2161 caagagaatt tccgagcgtt tgtgggcggg catatctgcc atcgaatctt ctgcgcgttc
     2221 cgctgcatcg atggcagcat cgacattctc tttgctggcc gttggcaccg tcccaatcac
     2281 tttaccatcg tatttatttt tcacttccat agtgggccct gagttgaccc actgtccgtc
     2341 gatcaaaagt ttatattcca ttttgcctca aaataagttt tcctcagtgg tcgagtgggc
     2401 attagcccgt accgagacca cgaattttca aaagtaaact tgtaacaaat acactttgct
     2461 tattggtggt ttcgatacgc ccttcgctac cgctcagggc tactcaacca ccgagctatc
     2521 aatgggcgat catcacgtgc ttcgtgatct gataatcacc gaccgcttcc gaggaaagat
     2581 cttttccaaa tcccgattgt ttgaagcctc cgtgaggggt ctcggaagtg atcggcaaat
     2641 ggtcgttgac ccaaaccgtg ccaaattcga gttggctggt caatctcatg gcgcgtccta
     2701 catcttttgt aaagaccgag gctgccaaac catacaacac atcgtttcca agatgcagcg
     2761 cctcggcttc attctcaaac tcctgcactg ttattactgg tccaaacact tcactttgca
     2821 cgatctccca atcctgccga acatcgctga tgacggttgg ctcatagtaa taaccctttt
     2881 gcaggtcctt tggaacaccc ccaccgacca ggatctttgc cccttgagat ttcgcccgat
     2941 cgacaaaacc agaaacgcgt tcgcgttgaa taccggaaat cagtggaccc atcactacat
     3001 catcctcgaa cggcaaccct acctttacat tcttcatcgc ctcaacgacc gcttcgcgca
     3061 tatcatttgc gcgtcccttt tccacataaa tgcgcgtcgc tgcggtgcaa tcctgacctg
     3121 tgttggcggt ggcggcaaag gaggctttcg cagcaaccag ttccgggtca gagtcatcaa
     3181 acacaataaa gggagcttta ccgcccaatt ccagatgaac acgtttgagt gtctccgcgg
     3241 cagtcttcat taccttctta ccggtgctgg aggctcccgt cacacagatc atgcggacat
     3301 ccgggtgctc tgccaaagcc tggcccgcat cattgccacc agaaataata ttgaccacac
     3361 cgtccggaat acctgcctcc tggatgagtt cagcaagcat gagcgtcgtc aacggtgtgc
     3421 ctggggcggg ctttaaaaca atcgtacagc ccgcggctaa ggccggtcca agtttccatc
     3481 ccgccatgtt caacggataa ttccagggtg tgatctgccc aatgaccccc actggctcac
     3541 gccgatagat ggaggtatag cctgccatga attcaccagc gtgtgatcca tgggtatcgc
     3601 gcgctgcggt ggcaaagaag cgcaggttat ccactgtaaa cggaatgtcg ctcgcaaggc
     3661 tgaggaattt atacggcttg ccggtatttt cgctttcaac gcgcgcgaat tcttctgagt
     3721 tggcttccag cagatcagcc agtttccaaa tggctttgga tcgttcgctt ggcgtcttct
     3781 ttgaccagcg accatcgtag aaggccttcc ttgcggcttg aacagcgcgg tcaacgtcgg
     3841 cgcgtgatgc gttctgcacc tccgcgatcg tttcgcccgt agccgggttc tcaatggaca
     3901 tctttccgcc gccttgtgta tccacccact gtccgtcgat ccaaagtcta tagtccatcc
     3961 gtgtttctcc ttgtggatat taccgggatt tcgcgattat acattccggc acccgtttgt
     4021 tcaacataat tctgccgaat tggataatga tcgtttgtga cgaagaaatc actcgtctcg
     4081 caaagctttt tcttcggcct tcggttttga accgatctcg accatttgca cctgaacctg
     4141 atagcgtttc tcgccaattt ggatttttcc cattccgaag aatccgcgcg agcccgtctt
     4201 gaactgtttt tcgggcgcgc tgagttcgcc tacaagtgtt ccattttctt tgatttcaat
     4261 aattagcatg ttatgctcct tcctttgtca tttctttgat gccatatgga aagccatagt
     4321 ctgccatttt ctgtaggaac ggcctagaat caaaggcctc cgggccgagt acgcccgagc
     4381 ccttccattg accatgctcg atcaagtcca tggcgatcac cggcgtgaac gcggtctgcg
     4441 cgacgactgc ctgacatccc cattgctcca tacaggcctg attatcggcc acctgataaa
     4501 gataaacttc ctttggttta ccgtccttac ttcctttgac ccaggtaccc gcgcatgttt
     4561 tgccatgcat cttttcgccc agatgtgcag ggtctggcaa tgatgcagcg accacatcgc
     4621 gcggtgatac ttcaacacct tttactttga tcgttccttt attgtccaaa cccaatttgt
     4681 gaagagtctt gaggactgaa atgaattcat cgcccagccc atacttgaaa gtgacacgct
     4741 ttgcttttaa ccagcgtgga accaacagga cttcttcgtg ttcgacattc accacatcca
     4801 gaggtccgat tccttctgga aattcgaagg tctcatgttc agaaaatggc tccgttgtga
     4861 accagccttt gccttcttcg aagatcaccg gcgggtttaa gcactcctca atggtggtcc
     4921 aaatggaaaa actcggggca aagtcatagc catcaattgt tagatttgca ccatcccgta
     4981 tgccaatctc ttcaatctca tcaaaaagat gatcctgtgc gtagcgggca aacacatcgg
     5041 ccatgcccgg ctctacacca aaaccgacaa gtgcgagcag atctttttct ttccaggctt
     5101 ctgctttatc gaactgataa tcaccaagct tgacattcgt catatggaac gggtcagttg
     5161 gatgcggctc agacaaggtc atcgccatat ccatataatt cgcgccgatg ttgtaggccg
     5221 cgtcgaaaat ctgcttgtta aaaatcggat ccactgcgtt catgatcagg tcgacatcat
     5281 attttttggc gagcgcttcg atattttcct gtttgcttgc atcaataaat tcgatcggga
     5341 accgcttcgc gtccgcgtcc cctaatttct tttgcacttc ttctgctcgc tggatgttgt
     5401 aatcagcaag caccattagt ttcatccact cacgtggttt tgcaatagcc gcgatcgctt
     5461 cccctactcc ccctaccccc acgagtaaaa ctcgcataaa ctcctttcaa atggctccac
     5521 cacccatcac gttgcggggg cagacaagtt tggtctaact ttattttgaa caggagacag
     5581 tcacccgctt cccgcggtga ctgtctcctt agcctaatct tatgaactgc ctacagcagc
     5641 tttgaattcg gtccagatac gatcgtagag ttctaatgcg ggaccaacat cttcaatcca
     5701 ctgtaaactt tcagtgactt ccgcactggg gtaaatcgac tcgttggaaa gcatttccgg
     5761 atcgatgaac tccttggcgg ccttgttggg ggttgcgtac ccaacccact ctgcattctg
     5821 cgcggcgatc tcaggatact ggaaatagtt catgaaaatc atcgccgtat acgggttggg
     5881 tgcacctgtc ggaatcatca tgttatcctg ccagatcgtg catccttcct gggggataac
     5941 gtattggatt tcttcacgtc caccagaatc tggcaggcca gcgagaattg catcaccggt
     6001 ccacatatgg ccgagtgccg tttcacctga aatgatcagg tcatcattgg tctggctatc
     6061 gtaggctttc acacaattcg attgtgcgat gagcaggttt ttcgcttctt caagatgggc
     6121 agggtcagta tcgttgatgg aataacccag cgacataagc gccgcgccaa ttgtttctcg
     6181 ctcgtcgtcc aacatactga tctggccaca ataaggtgag ttaggatcaa acatcatgtt
     6241 ccaggagggc tggacatcgg ttaccacact ggtatccact gcaaagccag aggtacccca
     6301 gaaccatgga acggtatatt tttgatcagg atcaaagtat tggttgacgt tgagcggctc
     6361 catgttctga atgttggtga ttacgttgta atccagttcc tgcaccatcc cctcctcgat
     6421 caaaagctgc accatatagt cagacggaac gatgacatcg tagtctgctc cgccagcctg
     6481 caatgtggct agcaaggttt cgttcgagtc atagttcgtc tcggtaactt ttacaccgca
     6541 ctcggtctca aactgtgcct tgaggtcggg gtcgatatat tcaacccagg tatagaggaa
     6601 aatctcatct gcaagttgag atgtatctcc acagcgatca gaagtctgat ctccgcccga
     6661 tgctggtgcc gttgcctcac ttgtcgcagg ttgacacgca gccagcatca aagacacaag
     6721 tacgaacatg taaatcagcg tctttttcat ttctttcttc tctcctttat taactatata
     6781 tctctcattc tccgaaagat gaaactgtta ctacgagttg tgtagcaggc atcacctcct
     6841 tagactatta gattaataga accgccgaca gaaatgctac agcggccgac acagacactg
     6901 cgatccaggc cagaattttt ccggaccgat ttgcctcatt caattcgtcg cgatagggac
     6961 cataagcccg ccgtacccag aggaacgcgg caattaacag caatcgaatg ataaggcctc
     7021 caattgtaaa ggacgcaaac aatgcaggca ggctaagcaa cccaaaaagc ccaagcccca
     7081 ggcctgcacc aaccgacatc gcacgcgtgt agacagcacc gccgttctga gcagtgagtg
     7141 aaaggccaac caagccaatt gaccctatca gtaatgccgt cgaaagtgca ttgatctcag
     7201 gcgtgacgcc gcgacgcacc agcgagtaga tatagagcgg catcgtttgt tcgcggatac
     7261 ccttggtgaa taccgtgatc aaataatcat ccagcgaaag agtaaaggcc atcaaagcac
     7321 cgccgatgat gccaggcatc agcaatggca gggtcactcg ctggaatgtt ttccattcat
     7381 ccgcgcctaa atctgcggcg gcctcttcga gcgtgcggtc aaaatccgcc aggcgcgcgc
     7441 gtacaataat atagacaaat ggaatatcga acgcgatgtg cgagatcgtg atcgtgtgca
     7501 atccggtgga agctctgatt ccgaaaacat tctgcaaaaa cggaaataga gcctgattaa
     7561 agaataacaa caaggcaatg cccatcacga tttcagggat cacaattggc aaatagagat
     7621 ttgcgtcgaa cgcagttcta agcctgaaac ggtaacgatc catggcaagt gcggtcaggg
     7681 ttccaattac agtggcaaaa atggtcgtaa tcaaacccac cagcaggcta ttccgcgccg
     7741 catctaacaa ttcagtattt gccgccaggg agacatacca ttctgtggaa aatccggtga
     7801 atacagcgac cgaacgcgtg ttattaaaag agaacagaat gagaatgatg actggcaggt
     7861 atagaaagcc aaataccagc gcggcattgg tccccaacaa ccagtttccg actctcgtgg
     7921 taaatcgcat ctgtcttgct tgctgtcggg tgacagctgg cgcatcggcg gtcatatgcc
     7981 cctccgtgtt tcttctgtac ttgagcggaa ataagcaatc acgccaatta acaccaatac
     8041 catcaagatg agcgacatgg atgcgccaaa cggccaatta cgcgacaccc cgaactggcg
     8101 attgatcaac gttccgatca tctcgatttt gccgccgccc atgatatccg gtgtgatgaa
     8161 ggcgcccacc accggcacaa agaccaagac cagggcagca atcacaccgg gtaaggttaa
     8221 tggtagcatc actttaagaa aggcacgtaa aggagaggct cccagatcag cagccgcttc
     8281 catcaacgta tgatcaaatt tttcaatcga ggcatacatt ggcaggatcg caaatggaag
     8341 gtagccatac accagtccta caatcacagc aaattcgtta aacaggagat ccagcggctg
     8401 accgattaaa ccagtattca tcagaaagga attgagcagt ccttctgtag cgagcacctg
     8461 cttgagcgca taggtacgca ccaggaaatt cgtccagaag ggaatgatta ccaggatcat
     8521 caaggcatcg cgccaaactt gtttctgttt cgatatgaaa taagagagtg gatagcctaa
     8581 aataatgcac caaaatgtga cgaccagcgc gatacgaacg gaatgcccaa agatgcctat
     8641 gtaaaggaaa ggatcgcact gaggtcccga aaattcagac gtgcaggcgt tgaataactg
     8701 aatataattt tccaacgtat acaccggcgg gatttgaatt cccccggcgc gtccacgttc
     8761 cacaaagctg ataaagagta cgattaagat gggcagattg aagaacagca aaatccacaa
     8821 cgcgccaggt ccaatcagac caaagagttg aaatcccttt ttctcacgga agaatttgag
     8881 cataatattc tccgccttac tccgtaagaa tgcgtgggga tattcgcgta aatgaaacca
     8941 tcacttcatc gccggtcttt gcaaactcat cattcgtatg tgcaatgttc tgctcgcgga
     9001 cacgcacctt ttgtccacct gcgaagcgta cgccaaagtg ggtatcggtt ccgatgtaga
     9061 tgacttcctc aacgcgtcct ttgagattgt tatcgccgct gcgctcgttg ttcaggcgaa
     9121 gcttttcggg tctcactgca accgacactt gctggccgtt tgtaaacttt cgactcgatt
     9181 ccacgaatac cgttccggta ttgtctattt caacttcaac caggttaccg ttctgcgcct
     9241 ttacaactcc gttgaggaaa ttcgtctcac caataaaatc ggctacgaat ttggtcgccg
     9301 ggcgttcata gatctcatca ggcttgccaa tctgcagggc cacgccttta ctcatcaccg
     9361 cgatgcgatc actcatcgtg agtgcttcct cctgatcatg agtgacgtag ataaatgtga
     9421 tccctacctc gcgctgcaac gttttgagtt ccaactgcat ttccttacgc agcttcaggt
     9481 caagggcacc gagcggttcg tcaagcagga ggacttcagg cttgttgacc agggcacggg
     9541 caagtgcaat gcgttgttgt tgtccgccag acatctgctt gggtttacgc atctccattc
     9601 cgggcagtcg gaccatgtcg agcgcttgtg aaacgcgcac tgaacgttcc gcgcgcggaa
     9661 cgcctttcat ctctaaaccg aactcgatat tctgttcaac cgtcatgtgt gggaaaagag
     9721 catactgctg gaacacggtg ttaactggtc gttgaaacgc tggcacctga ctcacatcct
     9781 tgccatggat caggattttc ccttccgtag gcatttcaaa gccggcgatc atacgcaggg
     9841 aggttgtctt tccgcacccc gatggaccta gcagggaaaa gaattcacca tccttgatct
     9901 gcattgtgac atggttgact gccgccaaaa tgccaccttc gggcgttgca aacttcttta
     9961 cgacatcttg catttcaact gcgtactcag ccatattgaa tgctcctcgg atgggatgag
    10021 tttattttat caacattcat gtcttttcga agaagtgatt cttttcatga aaccatctgt
    10081 ccgtgcgata agcgaactca cagcgttttg gctaccacac caactgcatc tgtcagacga
    10141 ttcagacctt cgtcgatttc ttcctgagaa ataatcaacg gcggttcgat gcgaatcgtc
    10201 tgtgcgctgg taagcgtgcc agctaccagc acaccgcgtt tgaacaattc cgccgcgatt
    10261 ttatagccta tctcgggtgt atgaaaatgc tggccgatga gcaatccctt gcctgttact
    10321 tctttataga tatgcggaaa ctcctgtgcg atatcatcca ctttctcttt gagatagacg
    10381 cccttggtgg cggcgctttc ccataatcgc tcacgcaaaa tgacattaat ggtagcgatc
    10441 gcggcggcgc atgccagtgc atttcctccg gttgtggttg tatgcataaa aggattcgga
    10501 tacatcatcg gctgaaagat ttcttctgta gtaatggccg tgctgaccgg cattacgccg
    10561 cctccaagcg atttggcggt ggcaaggatg tctggtgtga cgttccaatg atttacaccc
    10621 cacagttggc cggtccgccc caggcccgtt tgcacttcat cggcaatgag tagaacacca
    10681 tatttcttgg tcacgtcgcg cacacgcggc cagaa
//
LOCUS       DASTJP010000750         8848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1228323, whole genome shotgun sequence.
ACCESSION   DASTJP010000750 DASTJP010000000
VERSION     DASTJP010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228323"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..910
                     /locus_tag="VFQ23_21855"
     CDS             218..910
                     /locus_tag="VFQ23_21855"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HET9909307.1"
                     /translation="MMNRPYVFINVAMTADGKIDTIQRRGAPISSQRDKERVDQLRAE
                     SDAVMVGGKTLLDEDPKLTVKSEALRSERVARGLSPNPIKVCIVTKAEIEPNSAFLNA
                     GPSGIVIFTTRQTSNTQVSFFKARGVQVFVHDSERVNLPNALKTLKELGIRRLMVEGG
                     ATLNFELLHLGLVDEVTAYVAPMIFGGNTAPTMAAGSGVGRSEAIPLKLMHVEQWDDG
                     GVLLQYFLERSV"
     gene            907..1665
                     /gene="ribA"
                     /locus_tag="VFQ23_21860"
     CDS             907..1665
                     /gene="ribA"
                     /locus_tag="VFQ23_21860"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET9909308.1"
                     /translation="MTTTMHEKFDMLLESDPDHHCEGLGRDKVCVRLVAMAELPTRFG
                     EFHIIAFENNRDGKEHVAITKGDVIGTSDVPVRLHSECLTGDALGSLRCDCRDQLETA
                     LRKIGQMERGMLLYLRQEGRGIGLINKIRAYSLQDEGLDTVEANHALGFRDDERDYAV
                     AAHMLMSLKIQSVQLMTNNPKKIDQLTQYGIQVNGRIPHIMAPNEYNRFYLETKAAKS
                     GHMIDFRGKEHLREQYDDPIVEGMSEDQIGALNE"
     gene            1658..2341
                     /locus_tag="VFQ23_21865"
     CDS             1658..2341
                     /locus_tag="VFQ23_21865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9909309.1"
                     /translation="MNEDSTQKVIVITGANGALGKKTAEAFAARGHSLVLAGMDQSKL
                     DSLASDLKLPDDRFLTLAADLRDAGAVHAAAEAVSDKFGRVDALFHLVGGWVGGKTIP
                     ESSAEDLESMLGQHVWTTFYLFQSFGPKLASNGWGRVIIVSASTVPKPPEKRGIYSAS
                     KAAQENLVLSLSVELKQSGVTANIIQVKAIDVENKGTGTTPEEIVATMLYLFSDEAAK
                     VNGARIPVY"
     gene            complement(2397..3185)
                     /locus_tag="VFQ23_21870"
     CDS             complement(2397..3185)
                     /locus_tag="VFQ23_21870"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HET9909310.1"
                     /translation="MKPLRPLFFFFSILAIVSLACSFGGGNNTPPTQAVQPTQPPPPT
                     AVPPTEVVATEPPAPTEPPAPESQQFFTEEFDDPLSNDWSILTVTGSNDADPDKVTVE
                     AENGNLVWNFDSEYVYYYIFYEPFTYEDVQIEVRADNRGRNNNNISLVCRYDPDVGWY
                     EFNIANNGLYDIYYAEVDSSGDIGYNRITNGGSNAIKQGKEVNEYLVSCKGEELSLTI
                     NGDEVTTVSEKNFGLREGQAGVSVSSFNVLPILIEMDWIKISEP"
     gene            3364..4266
                     /locus_tag="VFQ23_21875"
     CDS             3364..4266
                     /locus_tag="VFQ23_21875"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9909311.1"
                     /translation="MELDANFYGTVKPIMPILALILLLTGAILHTAWNLILKQSKEKY
                     IATWWMLITGGVVALFALFLTGLPPREMWKFAIFSVITEATYFITLTYAYHDSEFSLV
                     YPIARGAAPAFLSLWSFVFLGERLTSGGMIGLGLIIGGLLIMGLSTLLKSNVSHVHFK
                     GIAIALLIALLISIYTTIDGAAVRHGADYALPYVMTMFALVPLPVTPFIIRQYGWARL
                     KAEWNELGLRVSIMGLLGVLSYLTAVLAFAFAPLSYSGAIREVSVVFGAFAGWWLLKE
                     KMGWMRVLGAIVIFAGILVIALFG"
     gene            4303..5118
                     /locus_tag="VFQ23_21880"
     CDS             4303..5118
                     /locus_tag="VFQ23_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909312.1"
                     /translation="MEITDTSGNKEGWIQNNIGFVLAVLALSCICLGSVIGAPLWGIN
                     DMKTTVLANATSTAFSIATQSNNVTVTAVARSTVQAGYEFIESFDEVSARWYVGPHPR
                     QYGDATYSIRDGVYIWDIRNSSTFFFWKNFYKGNKIKDFDAFLDTRFVESESQGAVCA
                     GLAFRSVEGDWNKGTFAFGICNDAHFQVQYYDRDGWREVSNSNLIDFIQVENWNRLEV
                     SARGDQFVFRINNMNVFEMTDDRLQQGSLGIYLVMPANESALIWFDNFGYQSR"
     gene            complement(5129..6547)
                     /locus_tag="VFQ23_21885"
     CDS             complement(5129..6547)
                     /locus_tag="VFQ23_21885"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET9909313.1"
                     /translation="MNPEEFRRAGHQLIDWIADYRAQVDKFPVRSKLEPGAVRAKLPT
                     APPVEPEPFEGIFSDLEEIILPGLSHFQSPNFFGYFPANGHLASVLGDYLSTGLGALG
                     LSWQSSPALTELEEVVTDWMRQMVGLSDAWSGVIQDTASTCTLLGLICARERASNYSL
                     SRGGLQGEAASLVVYVSQHSHSSVEKAALLAGFGRENLRFIDTDENFAMKSNALNEAI
                     LTDLEHGLVPCAVVATTGTTTTTALDPIEAIAKVVRQHKLWLHVDAAMAGSAMILPEC
                     RWMWDGIEEADSLVFNPHKWLGAAFDCSLYYVRDPQHLIRVMSTNPSYLQSAVDDKVK
                     NLRDWGIPLGRRFRALKLWCLIREQGVEKLQTRLRRDLANAQWFAEQVRSTPNWRVLA
                     PVPLQTICVRHEPQGLDGDDLDKHTQTWVERVNNSGQAYLTPATLDGRWMVRVSIGSL
                     LTERPHVVALWELIQREVKNSS"
     gene            complement(6727..7272)
                     /locus_tag="VFQ23_21890"
     CDS             complement(6727..7272)
                     /locus_tag="VFQ23_21890"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET9909314.1"
                     /translation="MSFYTSKGDDGTTNLLGEGRVAKYHARIEAVGTLDESTAALGLA
                     RAQALDPRSNEILLEAQRDLYKLMAEVAATPENAEKFRLIDAARVTWLEEQTDALSKL
                     VEMPKEFIVPGDSLAGAALSLARTVIRRAERRVVELFDAGELTNPDLQRYLNRLSSLC
                     FVLELLENKAAGHTTTLANRK"
     gene            7393..8037
                     /locus_tag="VFQ23_21895"
     CDS             7393..8037
                     /locus_tag="VFQ23_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909315.1"
                     /translation="MLTKYHHQILSEGLAERFSVRAFSAIVTANNHQDRLSGLLFHDE
                     YHVDNSAFQQANDYLEGQRSLTISSLKANDAKAAWAAFGRLTHTVHDFYAHSNYIDLW
                     LARFNDGRQFPSNSPSTPAEIDPVDPSLLHHSELHSGKVYYPLELFYFVKPLRKFALK
                     FIPRDSHAWMNLDSPEQGFKFDYAMQAAIKRTKIEFDKTTLGFSDEMCRLFLDK"
     gene            8242..8658
                     /locus_tag="VFQ23_21900"
     CDS             8242..8658
                     /locus_tag="VFQ23_21900"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9909316.1"
                     /translation="MTSNANQSDSSEAPSGNADTLRATEVSRLRALVDADMETADKLH
                     AEDFQLITPGGSALSKAEYLSGVASGELDYRLWDVDSPIDVRLFDEVAIIRYRSILHM
                     VYHGNDLGSDKYWHTDSYEWRDGRWQVVWSQATRIN"
BASE COUNT         2233 a         2126 c         2241 g         2248 t
ORIGIN      
        1 taacccaggt tgccacctcg atctccaaat cgcctcaccc cgactctgat gaatgtttgt
       61 cagaatagtt tctcctttct cccgctgaga gatggcaggc tatgcactgc gaccagaggt
      121 aggtgtcaaa gcggtgaggg tatttcgctc ctgaaactac acaacaaatt gaggcaattc
      181 attaataagt ctggttttga aactataatg gtcactgatg atgaaccgcc cttatgtttt
      241 tatcaacgtc gccatgactg cagatggcaa aatcgatacg attcagcgta ggggcgcgcc
      301 gatctcttcg cagagggata aggaaagggt ggatcaattg cgtgcagaat cagatgcggt
      361 gatggtgggc gggaaaaccc ttcttgacga agaccccaag ttaacggtta aatctgaagc
      421 attgcgctcc gaacgggtcg cgcgcgggct ttcaccaaac ccgatcaagg tatgtattgt
      481 aacgaaggca gagatcgagc caaattctgc atttcttaat gcgggaccct ccggtatcgt
      541 aatatttaca actcgacaga catctaatac ccaggtttct tttttcaagg cgcgcggcgt
      601 tcaggtgttt gtgcatgact cagaacgagt gaacctgccg aacgcgctga aaacgttgaa
      661 ggaacttggc attcgtcgtc tcatggtgga aggcggcgcc acacttaatt ttgaactatt
      721 gcatctcggg ctggtggatg aagttacagc ctatgtagct ccgatgatct tcggggggaa
      781 cactgctcca accatggcag caggttcagg cgtaggtaga agcgaagcaa tccccttgaa
      841 attaatgcat gtagaacaat gggatgacgg tggagtgcta ttacaatatt tcttggagag
      901 gtcagtatga ctacaacaat gcatgaaaaa ttcgatatgc tactggaatc cgatccagat
      961 catcattgcg aaggtttagg cagagacaag gtttgtgtac gccttgtggc aatggcggag
     1021 ttgccaaccc gcttcgggga attccatatt attgcatttg aaaataatcg cgacggaaaa
     1081 gagcatgtcg ccattacaaa aggggatgtg atcggcacgt ccgacgtgcc tgtgcgtctg
     1141 cattcggagt gcctgaccgg tgatgcgctg ggctccttgc gctgcgattg tcgcgatcaa
     1201 ttggaaaccg ccttgcgaaa gatcggtcag atggaaaggg gtatgctgct ctatctgcgt
     1261 caggaagggc gaggcatcgg tttgattaac aagatccgcg cctatagttt gcaggatgaa
     1321 gggctggata cggtcgaagc aaaccatgcc ctgggatttc gcgatgatga acgcgattat
     1381 gcagttgcgg cgcacatgtt aatgtcgctt aagatccaat cagttcaatt gatgacgaat
     1441 aatccaaaaa agatcgatca actcacgcaa tatggtattc aagtcaatgg gcgcatccca
     1501 cacattatgg ctcccaatga atataaccgc ttctatctgg aaacgaaagc agccaagtct
     1561 ggtcatatga ttgattttcg cggcaaggaa catcttcgtg aacagtatga tgatccaata
     1621 gtggaaggca tgagcgaaga ccagattgga gcgttgaatg aatgaggatt ccactcaaaa
     1681 agtaatcgtc atcaccggtg ctaatggcgc ccttggaaaa aaaactgcag aggcctttgc
     1741 ggcacgggga cactccctgg tgttggctgg catggatcaa agcaaactgg attctttggc
     1801 cagcgatttg aagttgcctg atgaccgatt tctcaccctg gctgcggatc tccgggatgc
     1861 aggagcagtt catgcggcag cggaagctgt ttccgataaa tttggcagag tcgatgccct
     1921 gttccatctc gtcggtggct gggtcggagg gaagaccatt cccgaatcca gcgctgaaga
     1981 cctggaatcc atgctgggcc agcacgtgtg gacgacgttt tatttattcc agtcctttgg
     2041 gccaaagctt gcctccaatg ggtggggacg tgtgatcatc gtttcggcct caactgttcc
     2101 aaagccgcca gaaaagagag ggatctactc ggcttcgaaa gctgcgcagg aaaatcttgt
     2161 tctctcactg tctgtggagc ttaaacaatc cggcgtgaca gctaacatta tccaggtgaa
     2221 ggctatcgat gtcgaaaata agggcacggg aacaacaccg gaggagattg tggcgaccat
     2281 gctatatttg ttctcagacg aagccgcaaa agtaaatgga gcaaggatac cggtgtatta
     2341 aaaacaaaac ttccgaagtc tcgtagactt cggaagttta ttagtcacat cttgaattac
     2401 ggctcgctga tcttgatcca atccatctcg atcaaaatcg gtaacacatt gaacgatgat
     2461 actgaaacgc ccgcctgtcc ttcgcgcaat ccgaaattct tttcagagac tgtcgtaact
     2521 tcatcaccat tgatggtcag tgaaagttcc tctcccttgc aggaaactaa atattcattg
     2581 acttccttgc cctgtttgat ggcatttgag ccaccgtttg tgattcggtt gtatccaata
     2641 tcgccgctgc tatcgacttc agcatagtag atatcgtata aaccgttgtt ggcgatgtta
     2701 aactcatacc atcctacatc ggggtcataa cggcaaacga gactgatatt gttgttgttt
     2761 cgtccgcggt tatcggcgcg cacctctatc tgaacatctt cataggtgaa aggttcatag
     2821 aaaatatagt aatagacgta ctcactatcg aaattccata caagatttcc gttttcggct
     2881 tccacagtaa ctttatcggg atccgcatca ttagaacccg ttactgttaa gatggaccaa
     2941 tcattggaaa gcgggtcatc aaattcctcg gtaaaaaatt gctgggactc gggagcaggt
     3001 ggctctgtgg gggcaggagg ttcggtagcg acaacctctg tcggaggtac cgctgtgggc
     3061 ggaggcggct gagtcggctg aacggcctgg gttggtggag tattatttcc accaccaaag
     3121 gagcacgcca ggcttacaat cgccaggatt gaaaaaaaga aaaacagggg gcgtaggggt
     3181 ttcatggtcc tctccattga aaaatttcga gtagtatagc atgggattgt tccaattcaa
     3241 tcacccgatg gttacacttt tgcaagcgtt tggcgagggg ttttctacca ccctattccc
     3301 tactcttgat tcaaaatctg cctttgctgc actctcccct tcactttgtt tcggagatgg
     3361 cgcgtggagc tggatgcaaa tttttatggt acagtcaagc ccattatgcc cattcttgcc
     3421 ctcattctcc tcctcaccgg tgccatactt cacaccgcct ggaatctcat tctcaaacaa
     3481 tccaaagaaa aatatatcgc cacctggtgg atgcttatta cgggaggggt cgtggcgctt
     3541 tttgccctgt ttttgacagg attacctcca cgcgaaatgt ggaaatttgc aatctttagt
     3601 gtaatcaccg aagccaccta tttcatcact ctcacctacg cctatcatga cagcgaattc
     3661 tcgctggtat atcccattgc gcgtggggcc gcgcctgcat tcctgtcgtt atggtccttc
     3721 gtgtttctcg gtgaacggct gaccagcggt ggcatgatcg gactcggctt aattattgga
     3781 gggttactaa ttatgggtct gagcacatta ttgaaaagca acgtcagcca tgtgcacttt
     3841 aagggaattg cgattgcctt actgattgcg ctcctgatct cgatctatac gacaattgat
     3901 ggtgcagcgg ttcggcacgg tgcagactac gccctgccgt acgtgatgac gatgtttgct
     3961 ctggtccctt tgcccgtgac gccattcatc atacgccagt atggctgggc gcgtctcaaa
     4021 gcggaatgga acgagctggg actgcgcgta tcgatcatgg gactgcttgg cgtattgtct
     4081 tatctaacag ccgttttggc gtttgccttc gcgccgctaa gttattcagg cgccatcaga
     4141 gaggtaagcg tggtattcgg cgcgttcgca ggctggtggc tcctgaaaga aaaaatggga
     4201 tggatgcgcg tgctgggtgc gatcgttatt tttgcaggga tactagtgat tgccctgttc
     4261 ggctgacgta gcctaatctt atgatccgtc cgtagggatt ttatggaaat tacagacaca
     4321 tctggcaata aagaaggttg gatccaaaat aatatcgggt ttgtccttgc agtcctggct
     4381 ttgtcctgca tttgcctggg tagcgtgatt ggtgcgccgc tttgggggat caatgacatg
     4441 aaaacaactg tgctggcgaa tgccacgtcc accgcgtttt ccatagccac tcaatcgaac
     4501 aatgtcacag tcaccgcggt ggctcgcagt acagtacagg cagggtatga attcatcgaa
     4561 agctttgatg aagtttcggc acgctggtat gtgggaccgc atcccaggca atatggagat
     4621 gcaacgtatt ccataagaga cggggtttat atatgggata ttcgtaattc cagtaccttc
     4681 tttttttgga aaaattttta taaaggaaat aaaatcaagg actttgatgc tttcctggat
     4741 acccggtttg tggagagcga atcacagggc gctgtttgtg cggggttggc ttttcgaagc
     4801 gtagagggtg actggaataa aggtacattt gctttcggaa tatgcaatga cgcccatttc
     4861 caggttcaat actatgatag agacggatgg agagaggtta gtaattcgaa tttaatagat
     4921 tttattcaag tagagaattg gaaccgcctt gaagttagcg cgcgagggga tcaattcgta
     4981 ttcaggatca ataacatgaa cgtctttgag atgaccgatg atcgcttaca gcagggtagc
     5041 ctggggattt acctggtgat gccggcgaac gagtctgctt tgatttggtt tgataacttt
     5101 ggttatcaaa gccgctaatt cgatttcgct acgaactatt tttaacttct cgctgtataa
     5161 gctcccataa agctaccaca tgtggacgtt cggtcaacaa tgagccaatg gatacgcgca
     5221 ccatccacct tccatccaaa gttgcggggg tgagataggc ttgaccggag ttattcactc
     5281 gttctaccca tgtctgtgtg tgcttatcta aatcgtcccc atccaggccc tggggctcat
     5341 gccgcacaca aatcgtctga agaggaaccg gagccagaac gcgccagttc ggagtagacc
     5401 tgacctgttc agcgaaccac tgtgcattgg cgaggtcgcg tcgaaggcgc gtttgtaatt
     5461 tctccacgcc ttgttcccga atgagacacc acaacttcag cgcacggaag cggcgtccga
     5521 gtgggatgcc ccagtcacgc agatttttca ccttatcgtc gactgcgctc tgtaaataac
     5581 ttggattcgt actcatcacg cgtatgagat gttgcggatc gcgcacgtaa taaagagaac
     5641 aatcaaacgc cgcgcccagc catttgtgtg ggttgaacac gagcgagtct gcttcttcga
     5701 ttccgtccca catccagcga cattcaggca ggatcatcgc ggaaccggcc atcgccgcat
     5761 cgacatgcag ccatagctta tgttggcgta cgacttttgc aattgcctcg attgggtcaa
     5821 gcgcggtggt cgttgttgta cctgttgtcg caacaacagc acatggaaca agaccatgct
     5881 cgaggtctgt tagtatcgct tcattcaggg cattagattt catcgcgaaa ttttcatctg
     5941 tgtcaatgaa gcgcaggttc tcgcgcccga acccggcaag caaagcggct ttctccacag
     6001 agctgtgact atgctgggaa acataaacga ccagggaggc cgcttcaccc tgcaagccgc
     6061 cgcgcgacaa cgaatagttc gatgcacgtt cacgagcaca aattaacccg agcaaagtac
     6121 atgtcgaggc cgtgtcttga atcacaccac tccacgcatc agagagaccc accatctggc
     6181 gcatccagtc tgtgactact tcctccagtt cagtgagtgc cgggctggat tgccacgaga
     6241 gtccaagtgc acccaacccc gtgctgaggt aatctcctaa gacagaggct aaatgtccat
     6301 tcgctggaaa gtaaccgaag aaatttggag attgaaaatg agatagacct ggcagaatga
     6361 tctcttctag gtcgctaaag ataccttcga atggttcagg ctctacagga ggcgccgttg
     6421 gaagttttgc gcgcactgct cctggttcaa gtttagaccg aaccggaaat ttatccacct
     6481 gggcgcggta atctgcgatc caatcgatca attggtgtcc ggcacgtcga aattcttcag
     6541 ggttcatagg ggattctctt tttaaaaatt attcgcgctg agcggagtga gagccatgct
     6601 cgtcgggtca catgaagacg aagcgcggcg gcttttgtgt ctatgataat cacccttcgt
     6661 cttcgggtct tgatacgagc gatgagaacc ttcgctctac tcaaccctcc gctcaggacg
     6721 agtggtttat ttacgatttg ccaacgtcgt tgtatgcccc gcggccttgt tctccagcaa
     6781 ctctaaaaca aaacacaaag acgataaacg atttagatag cgctgcaaat caggattcgt
     6841 cagttccccg gcgtcaaaca attcgactac gcggcgttct gccctgcgaa tgaccgtccg
     6901 cgccagagaa agcgctgcac ccgccagaga atcgcctggc acaatgaact cttttggcat
     6961 ctcgaccagt ttgctcaaag cgtcggtttg ttcttcgagc caggtaacac gcgcggcatc
     7021 aatcaaacgg aatttctctg cgttttcggg ggtagcggca acctctgcca ttaacttata
     7081 tagatcgcgt tgtgcttcga gcaggatttc gttcgaacgt ggatcaagcg cctgagcacg
     7141 cgccagccca agagcggcgg tggattcgtc cagcgtcccg accgcttcga tgcgggcatg
     7201 atactttgct acacgtccct caccgagcag attcgttgtc ccgtcatccc ctttcgaggt
     7261 gtaaaaactc atgcaatgat tataagcgaa gcgaaccctg tgggtaagcg aagcgaaccc
     7321 tgtgggtaag cgaaccctac gagtaacccg tggcttattt acagaatagg cttggcttat
     7381 aatcaatcaa ttatgctgac gaaatatcac catcagatcc tctccgaagg actggcagag
     7441 cgttttagtg tgcgcgcttt ctcagccatt gtcactgcaa acaatcacca ggatcgatta
     7501 agcggacttt tgtttcacga tgaatatcat gtagataaca gtgctttcca acaagccaat
     7561 gactatctcg aaggacaacg atcactgacc atttcttctc tcaaagcgaa cgacgcgaaa
     7621 gctgcctggg ctgcctttgg acgattgact cacacggtgc acgattttta cgcgcacagt
     7681 aactacatcg atctgtggct ggcgcgcttc aacgatggga ggcaattccc atcgaattcg
     7741 ccttccacgc cagcagagat tgatcccgtt gacccgtctt tgctccacca ctcggagctt
     7801 cattctggaa aagtctatta cccgctggag ttattttatt ttgtcaaacc gctcaggaaa
     7861 ttcgcactca agttcatccc gcgcgactca cacgcctgga tgaacctcga ctcgcccgaa
     7921 cagggattca aattcgacta cgccatgcaa gccgccatca aacggacgaa gatcgagttt
     7981 gataaaacga cattgggttt ttcggacgaa atgtgcaggc tgtttttgga taagtagaca
     8041 tgggagccta taattagctt gttgaacatt aatctttcgc cgcctaaccc tacaatttgg
     8101 gatgttatac ggcgtccgtc gaatctctag ttcggcggtt actccgaaag attattgaga
     8161 attaccgatt ttttatctgt aagaagttgc acgcgaatat ttaaccacgt aagtccctat
     8221 tagtagaatg gatgtagcga tatgacaagt aacgcaaatc agtcagattc ttcagaggcg
     8281 ccaagtggaa acgctgacac attgcgcgca acagaggtta gccgactccg agctctcgta
     8341 gatgcggaca tggaaactgc tgataaattg cacgcagaag attttcagct cattacacct
     8401 ggtggatctg ccctgtcaaa agcagagtac ttgagcggag ttgcctcggg tgagctggac
     8461 tatcgcctgt gggatgtcga ttcgccaatc gatgtgcgcc tgttcgacga agtcgccatc
     8521 atccgctacc gatccatcct tcacatggtt taccacggta atgaccttgg atcagataag
     8581 tactggcaca cagacagtta tgagtggcgc gatggccgtt ggcaagtggt gtggtcccaa
     8641 gcgacacgaa ttaattgatt acgttgtcgc cgtttcactg caagataaca aaagagaatc
     8701 accgcctaac acagcgtact aagaaacaga attacggtag aaaacaatga aagatgtgca
     8761 agctgcataa ccccaaaaag gcgtcgcgac aatttccaaa aaacgacgcc gatgagatgc
     8821 tagaattgtt cgacatgaac ggcacggc
//
LOCUS       DASTJP010000751         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_912053, whole genome shotgun sequence.
ACCESSION   DASTJP010000751 DASTJP010000000
VERSION     DASTJP010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912053"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFQ23_21905"
     CDS             <1..413
                     /locus_tag="VFQ23_21905"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9909317.1"
                     /translation="VADSKVEQAKAALEVLQASIAQNIVTAPHDGTIASVNVAAGEFV
                     QTGQVVLTLATLDQFQIETTDLSERDIANVQVGDTVNISFDALETTVRGKVASISPKA
                     DTVGGDIVFKVTIALDEQLKGLLWGMTAEVTIGE"
     gene            705..2099
                     /locus_tag="VFQ23_21910"
     CDS             705..2099
                     /locus_tag="VFQ23_21910"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909318.1"
                     /translation="MPLPTKITVIGAGSASFGENTLSAIMRSKKLRGSTLALVDRNAQ
                     SLEIVHRLANRLNREWDAQFNLTAHTDHRDALADSDFVVVAIEVGAREGLWKQDFEIP
                     IKYGVRQPYSENGGPGGFAHAARNIGPIMQIARDMEQACPQAWLVNFTNPMIRICDAV
                     NRYSSIKAVGLCHQIYAGYGMVGVALAKDLDIEVPDGLTGMHAAMDQHPLQHRVKEQI
                     VPLINIRAAGLNHFTWILSIQDRRTGEDLYPLFRKRFFELDPSFEPLTRDIFSAFGLF
                     PVPGDTHLCEYLPWMSDPVTKPWERYNIRLYDWDLFASIRDYSLDRLNDMSNGHATIE
                     GLLETDSEGALEMIENIAGGGTHYHLAANLPNSGQISNLPLRAIVETPIIVDGSGIHP
                     VHVGALPDGIAELCRRETTVAQLSVDAAVEGNREKALQCFLLDPVTTDIDIARKILDD
                     YLTSYKEYLPQFWQ"
     gene            2186..>2887
                     /locus_tag="VFQ23_21915"
     CDS             2186..>2887
                     /locus_tag="VFQ23_21915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9909319.1"
                     /translation="MHDHNQHDDHDHDHDHDHEHEHSRNPIVEWFKHTFAPHDHGYQT
                     AALDAALATDRGIWAVKISLVALLVTALFQVVIVAISGSVALLADTIHNFSDALTAIP
                     LGIAFTLSRRARNTRFTYGYGRAEDIAGVIIVIMIFATAVEAIRQSVLKLLDPQPISN
                     LGWVAVAAVIGFLGNELVALYRIRVGKQIGSAALIADGNHARTDGFTSLAVLAGVIGV
                     WLGYPLFDPIVGLGIG"
BASE COUNT          708 a          698 c          760 g          721 t
ORIGIN      
        1 aggttgcaga ttcaaaagta gagcaggcaa aagctgcact ggaagtcttg caagccagca
       61 ttgcccaaaa catagtaact gctccccacg atggaacgat cgcgtctgtg aacgtagccg
      121 cgggtgagtt tgtacaaacg ggtcaagtgg tgctcacact ggctacgctg gaccagttcc
      181 aaatcgaaac gacggattta agtgaacggg atatcgcaaa tgtgcaggtg ggcgataccg
      241 tcaatatatc gtttgatgcg ttggagacaa cggtacgtgg aaaagtggcc agcatttcac
      301 cgaaggctga tactgtaggt ggtgatattg tgttcaaagt gacgattgca ttggatgaac
      361 agctaaaggg attgttgtgg ggtatgaccg ccgaagtgac gattggtgaa taggcgagag
      421 attcaaatta ttaaaagaca cggagatgaa aatcatcccg tgtctttttt tattaacctt
      481 tctatggtta aatcgacttc ataaagaaac ggtggtcgag tagccccgaa tgattctgga
      541 ggggcataca cttgcacctg cgtgccagtg caggtgtcga gaccaccagt tagcaaagaa
      601 acttctgcta taaaaggact tttgaaagtt gttggtctcg agacgccttt cgcagcaatc
      661 gctccggcta ctcgaccaac ggctgaaact ggagaactaa atacatgcct cttcccacaa
      721 aaataacagt gattggcgca ggctccgcgt catttggcga gaacacgtta tctgcgatca
      781 tgcgctcgaa aaagctgcgc ggctccacgc ttgctctggt ggatcgaaat gcgcaaagcc
      841 tggagatcgt tcatcgcctc gccaatcggt tgaaccgcga atgggatgca cagttcaacc
      901 tcacggcaca taccgaccac cgggatgcac tggcggatag cgactttgtc gttgtcgcca
      961 ttgaggtcgg tgcgcgcgaa gggttgtgga aacaagactt tgaaatcccc attaaatatg
     1021 gcgtgcggca gccttattca gaaaacggtg gacccggtgg ttttgcccat gccgcccgca
     1081 acatcggtcc catcatgcag attgctcgcg atatggaaca agcttgcccg caggcctggc
     1141 tggttaattt tacgaatccg atgatccgca tctgtgacgc ggtcaatcgt tacagcagta
     1201 tcaaagctgt cggtctctgt catcagattt atgcaggtta tggaatggtc ggtgttgcac
     1261 ttgcaaaaga tcttgatatc gaagtcccag atgggctgac cgggatgcat gccgctatgg
     1321 atcaacatcc gttacagcac cgtgtcaagg aacaaatcgt accactcatt aacattcgtg
     1381 cagcagggtt gaatcacttc acctggattc tttccattca ggatcgccgt acaggcgaag
     1441 acctctatcc gttgttccga aagcgcttct tcgagctgga cccgagtttt gagccgctca
     1501 cgagggacat tttttcagcc tttggattat tccctgtgcc tggcgataca catctgtgtg
     1561 aatatctgcc gtggatgagc gatcccgtga ccaagccctg ggaaagatat aacattcgtc
     1621 tttatgattg ggatctgttc gcctccatcc gcgactacag ccttgatcgt ttgaacgaca
     1681 tgtcaaacgg acatgcaacc atcgaaggct tactcgaaac tgattcggag ggtgcgttgg
     1741 agatgatcga aaacatagca ggcggcggga cgcattatca tctcgcggcg aatcttccga
     1801 acagcgggca aatctcgaac ctccctctcc gtgccattgt tgagacgcct atcatcgtag
     1861 acggttccgg gattcacccc gtgcatgtgg gcgctctccc tgatggaatc gcagaactct
     1921 gccggagaga aacaaccgtt gcacaattaa gtgtggatgc cgcggtcgaa ggcaatcggg
     1981 agaaggccct gcaatgcttt ttgcttgatc ccgttacgac agatattgat atcgccagga
     2041 aaattttaga cgactatctg acgagttata aggaatacct gccgcagttc tggcaatgag
     2101 catattcttg cgagcgagct gaaggcgagc gtggcaatct ccaagaacct gtaaacatct
     2161 atttgaatga ataatcgagg tatctatgca cgatcataat caacacgatg atcatgacca
     2221 tgatcatgat cacgaccatg aacatgaaca ttccaggaat cccatcgttg aatggtttaa
     2281 gcacacgttc gctccgcatg accatggata tcaaacagcg gcgctggatg ccgcgctagc
     2341 caccgaccgc ggcatctggg ctgtgaagat ttcactggta gctttgctgg ttactgcact
     2401 gttccaggtg gtcattgtgg cgattagtgg aagtgttgcc ctgctggcag atacgattca
     2461 caatttttca gatgcgctta ccgcaattcc acttggcatt gcttttactc tctcgcgccg
     2521 cgcacgcaat acacgtttta cctatggcta tggacgtgca gaagatatcg cgggtgtcat
     2581 tattgtgatc atgatctttg ccacggcagt ggaggcaata cgtcagtccg tcctaaaact
     2641 gctggaccct cagcccattt cgaatctcgg atgggtggca gtcgccgcag tgattggctt
     2701 tctggggaat gaattggttg cactctatcg catccgcgtt ggcaaacaga ttggctctgc
     2761 tgcgttaatc gcagatggta accatgcccg cacggatggc tttacctcgc ttgcagttct
     2821 tgccggtgta atcggagtct ggctaggcta tccattgttt gacccgattg ttggtttggg
     2881 gattggc
//
LOCUS       DASTJP010000752         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1018302, whole genome shotgun sequence.
ACCESSION   DASTJP010000752 DASTJP010000000
VERSION     DASTJP010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018302"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /gene="zwf"
                     /locus_tag="VFQ23_21920"
     CDS             complement(<1..1444)
                     /gene="zwf"
                     /locus_tag="VFQ23_21920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET9909320.1"
                     /translation="MENSLPISIVIFGASGDLTQRKLVPSLFNLFRKGRLPKQFRIVG
                     YGNTAFSEEQFRTHLKEGLTEFASFKYEDEEWSNFASNLAYQQGRYTDLADFKKLGNL
                     LKSWEISSGNRIYYMATPPGVFPNIIDLLGLTDQLTEYNGWRRVVIEKPFGTDLESAR
                     SLNEQIHKTLHERQIYRIDHYLGKETVQNILMTRFANTIFEPLWNRNYIDHVEITVAE
                     QVGVEHRGRFYDHVGVLRDMFQNHLLQLTSLVAMEPPASFDATALRNEKVKVLSSIRP
                     MSEEEVLKWTVGGQYSGYREEEGVNPNSVALTFASLRLQIDNWRWQGVPFYLRSGKGL
                     KEKLSQITIEFKKPPHLLFPSTDGDLTANMLVLFLQPHEGVHWRFEAKVPDTVAKLRS
                     VDMEFHYEEAFGQTALPDAYERLLLDTITGDASLFTRADEVETAWSLIDPITKAWEAQ
                     KRPLAFYEPGSWGPKESDDLLAADGRKWSTW"
     gene            complement(1535..2467)
                     /gene="gnd"
                     /locus_tag="VFQ23_21925"
     CDS             complement(1535..2467)
                     /gene="gnd"
                     /locus_tag="VFQ23_21925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET9909321.1"
                     /translation="MTNQQGGIMELAMIGLGKMGLNMATRLARGGHRVIGYARTAATV
                     DEAIKLGAEGAHSLEEVVSKLNAPRIAWVMVPAGQVTYDTIEKLSNLLSSGDIVIDGG
                     NSNYKDSVRHAAMLEPKGIDFVDCGTSGGIWGLTEGYSLMIGGQAAVVEKMRPIFETL
                     APGPDKGWGHVGPHGAGHFVKMVHNGIEYGMMQAFAEGYSILKAKKEFELDLAQISHI
                     WQHGSVVRSWLLDLAARALDEDAKLSDIKPWVADSGEGRWTVFESIDLDVAAPVITLA
                     LQMRFLSRDEENFSARMLAALRNQFGGHAIKKAE"
     gene            complement(2628..>3069)
                     /locus_tag="VFQ23_21930"
     CDS             complement(2628..>3069)
                     /locus_tag="VFQ23_21930"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET9909322.1"
                     /translation="ELPPVEASKDYSSTLKEFASPPFDWPRFDLLYLGMGEDGHTASL
                     FPGSPVTVSEPTVPVTAHYQDRPANRVTLTQLVFNQARCIVFMATGEKKSFTLAEVLS
                     DRYNPELYPAQRIDPKDGELIWLVDEDAASKLPRKIKGLKICEG"
BASE COUNT          734 a          798 c          734 g          803 t
ORIGIN      
        1 cccaggtaga ccactttctc ccatctgcgg cgagtaagtc atccgactct ttgggtcccc
       61 aggaaccagg ttcgtaaaaa gcgagtggtc gtttttgcgc ttcccatgct ttggtaatcg
      121 gatcgatcaa agaccaggcc gtctcgactt catccgcgcg agtaaaaaga gatgcgtcgc
      181 cagtgatggt atccaatagg agacgttcgt aggcatccgg aagcgccgtc tgaccaaagg
      241 cctcctcata atgaaattcc atatcgaccg agcgtaattt cgcaaccgtg tctggcactt
      301 ttgcctcgaa acgccagtgg acaccttcgt gtggctgaag aaagagcaca agcatgttag
      361 ctgtcagatc accatctgtg gaaggaaaaa gcaaatgagg cggtttctta aattcgatgg
      421 tgatctgaga taatttttct ttcaagcctt tgcctgagcg aagatagaaa ggcacaccct
      481 gccagcgcca gttgtcaatt tgtaggcgca ggcttgcgaa ggttaatgcg acggagttcg
      541 gattaacgcc ttcttcttcg cggtaaccac tatattggcc gccgaccgtc cactttagca
      601 cttcttcttc agacattggg cggatcgagc tcaacacttt gactttttca tttcgaagcg
      661 cggtggcatc aaaggaggcg gggggctcca tggctaccaa ggaggtaagt tgcagcaggt
      721 gattttggaa catatcacgc agcacaccga cgtggtcata gaaacgtccg cggtgctcca
      781 cgcccacttg ttcagcaacg gtaatctcta catggtcaat ataatttcga ttccacagcg
      841 gctcaaaaat cgtgttagca aagcgtgtca tcaggatatt ttggacagtc tctttgccca
      901 agtaatggtc aatgcggtag atctgtcttt catgcaatgt cttatgaatc tgttcattca
      961 aactgcgcgc agactcaagg tctgtgccga aaggcttttc gattacgacc cggcgccagc
     1021 cgttgtattc ggtcaattga tccgtcagcc cgagcagatc aatgatattt gggaaaacgc
     1081 cgggaggtgt ggccatgtaa taaatgcgat tgccagaact aatttcccaa ctctttagaa
     1141 gattgcccag tttcttaaaa tcagcgagat ctgtatagcg gccctgttga tacgcaaggt
     1201 tcgaagcaaa gttagaccat tcttcgtctt catatttgaa gctggcaaat tccgttagcc
     1261 cttctttgag atgtgtgcgg aactgctcct cagaaaaagc ggtattgccg taaccgacga
     1321 tgcggaactg ctttggcagg cgccctttgc gaaataaatt aaataacgat ggcaccagct
     1381 tgcgctgtgt aagatcgccc gatgcaccga agattacgat agaaatgggt aagctgtttt
     1441 ccatatttta tattgtctcg ttctgccgcc gagtctcaga gacacagaga aagaattgat
     1501 tctccgcgac tccctgtctc tgtggcgaat tcatttattc tgctttcttt attgcgtgcc
     1561 caccgaattg attgcgtaag gcggccagca tgcgtgctga gaaattttct tcgtctcggc
     1621 tgaggaaacg catttgcaaa gccagggtga tgacgggtgc tgctacgtcc aggtcaattg
     1681 actcaaatac ggtccaacga ccttcccctg aatctgccac ccagggtttg atatcagaaa
     1741 gctttgcatc ctcgtccagc gcccgtgctg caagatccaa caaccaggag cgcaccacac
     1801 ttccatgctg ccagatgtgt gagatctgtg cgaggtccaa ttcgaactct ttcttggctt
     1861 tcagaatgct gtatccctca gcaaatgcct gcatcattcc atattcgatt ccgttatgga
     1921 ccattttgac gaagtggccc gcgccatgcg gtcctacgtg accccaacct ttatccggac
     1981 ctggagccag agtctcgaag atgggacgca tcttttcgac caccgcagcc tgcccaccga
     2041 tcatcagact gtatccttcc gtcaatcccc agatcccacc gctggtgcca cagtcgacaa
     2101 agtcgatccc ttttggttcc agcatcgcgg cgtgacggac cgaatcttta taattggagt
     2161 tacctccatc aatcacaata tcgccgctcg aaagaagatt tgataatttc tcaatcgtgt
     2221 cgtatgtgac ctgacctgcc ggaaccatta cccaggcaat acgcggcgca ttgagtttac
     2281 tcaccacctc ctccagcgag tgcgcgcctt ctgcacccag cttgatggcc tcgtcaaccg
     2341 tcgcggctgt acgcgcatac ccgatgacgc gatgtccgcc acgtgcaagg cgtgttgcca
     2401 tgttcaatcc catcttgccc aagccgatca ttgccagttc cataatgcct ccttgttgat
     2461 ttgtcattgc ggggagccca aagggcgacg tgacaatctt gtgttttcac gtaacatttt
     2521 atttataggt tactttatac acttgcagcg gcgagtgatt tgcttggcaa atcatgcagg
     2581 tgttgcttcg gacgaacgat caccgatcta acaatgacat cggttaatta accttcacaa
     2641 attttcagcc cctttatctt ccttggcaat ttacttgctg catcttcgtc aaccagccag
     2701 atcaattcgc catctttcgg gtcaattctc tgtgcggggt atagctccgg attatatctg
     2761 tcacttagca cttctgccaa agtgaatgac ttcttttcgc cggttgccat aaatacaata
     2821 catctcgcct gattgaacac aagctgggtc aatgtgacgc ggtttgccgg gcggtcctgg
     2881 taatgagccg tgacgggtac ggttggctct gatacggtca caggagagcc agggaataac
     2941 gaagccgtat gaccatcttc tcccatccct aaatacaaaa ggtcaaagcg gggccaatcg
     3001 aaaggagggg aggcgaattc tttcagtgtg gatgaataat ctttcgaagc ctcaactggc
     3061 ggaagttcg
//
LOCUS       DASTJP010000753         5967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1271316, whole genome shotgun sequence.
ACCESSION   DASTJP010000753 DASTJP010000000
VERSION     DASTJP010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271316"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2918)
                     /locus_tag="VFQ23_21935"
     CDS             complement(<1..2918)
                     /locus_tag="VFQ23_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909323.1"
                     /translation="MECPNCKHTTGNSVLLQCSHCGEAFERRLLEEYQHLQYLAGWLG
                     DRPEISPDLRGQLLTLVGAKQNLLRGQLLPKVSQKEKPVQPKPSPIPVAEKKPATEKA
                     IPAPVVSAKAESAPLPTAVVKPIPVAVAAPKPKPAPPKPVAPPTPPRPPIDWRKLILE
                     AATSGALLRALLYLGAFMIVVSATVLVIRFWNQFHPIIQLLFIASVPLSFYAGGWALR
                     VRLKLEQAGTVLTGIGALLVIVDFGAIYQLGGIGQNNGPLYWLFVSIFCTALYTFTAW
                     KLKGEFFDYLPLLAATSTLFTFTRLLRLDLEWSVVSVTVSGTLMTMFAGQYSKANGQW
                     REFARAARYLSQILIPASLFYIVFSPGMPPVGQMMSFMFATAGYLVLAWQFPAFVFAY
                     AAIGGSIGTVIFALRVGGLGFEWYPAAVSVLAMAYFLISERTRHLKIDPLIIEKYAAA
                     LNVSGLLLLSIGAVGGFIVSVGNQVWGGILAMIFSTLDLILCAYLFRKSRYTLLASGL
                     FIAPVSMTTIELLKGMQLQIPAAIAWITVAWGALAIFYIILAAVFNGLEKHNRWLYEW
                     AHILTLLALFCLPFSYLLDPTNWTKIPTLVSLGACILVYLVSFVLQDSGRHASLSPLS
                     TWLPYGLGKGIFLWPVALLTPFWFAVAGNSLNFNNPRVGVVLTGVGLAYIGIGQILIR
                     RAREYRLPFHTLTYFLCMLGIFISLPNIYALSTALLITVAAASVIAYLYNRLIETIIA
                     GVLFIWPFQLSMEMINISDHVQTLAYALLASFVYIPVAIQLNKFQMSREKSHPLPIFS
                     VGYALMGYAIIQSLLQSQSNHYIPWIGVAVSLVGTALFTFSASYFRKSRLAFLWASAS
                     ILTFTIAFGQALILFKIPSAYNALAWVGLAFFYVIAERTVFFRPRKQEQEIENMFRAP
                     VIVSASILSILALSLTLPPTINTLVLAGVLPAEILPAILAQVAVVLLAVLSARLYKQG
                     "
     gene            complement(3105..4592)
                     /locus_tag="VFQ23_21940"
     CDS             complement(3105..4592)
                     /locus_tag="VFQ23_21940"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HET9909324.1"
                     /translation="MNIPDWVQDAVFYQIFPDRFARSARNPNDTLTFEPWDAPPTVHG
                     FKGGDLYGVVERLDYLKDLGITALYLNPIFESASNHRYHAYDYYTVDSILGGNRAFTE
                     LLEKAHRKNIRIVLDGVFNHASRGFWQFHHVLECGEDSPYKDWFLFDPERLNRKKHWG
                     AYPSPHEQRLLQHEDSITAIGYRGWWNLPALPKFNTKSPVVREFLFDVAEHWIKLGVD
                     GWRLDVPTEIDDDSFWQEFRLRVKNINPEAYIVGEIWHEAQRWLQGDQFDAIMNYDIA
                     RPCLAFFSGRHLDLKILHQQLNYQGIHYPIDSDEFANRIDYNLQLYKREVTYSQLNSL
                     NTHDTPRFLSCAGGDKNALKLAWMFIFAYPGAPNIYYGDEIGVDGKHDPDNRKSFPWD
                     ESKWDKDLLKYAKDLIKLRRKNPALRRGDFKRLWSANGVYAFSRSLDQQTFIIALNAS
                     ESAQQAEVKYEARNPKVLYGEASNLSVNDQRLRFTIPARSGVILR"
     gene            complement(4726..>5967)
                     /locus_tag="VFQ23_21945"
     CDS             complement(4726..>5967)
                     /locus_tag="VFQ23_21945"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9909325.1"
                     /translation="WYLWRDPAPDGGPPNNWLSHFGGPAWTFDQTTGQYYLHQFVKQQ
                     PDLNYRNPDVVTAILDVMRFWLDRGVDGFRVDVIGLMMKDTEFRDEPLNSAWDGVNPF
                     GQLNHIYTANLPEVHELIHEMRKVLDSYDDRMMVGETYLPNDMLMKYYGTADQLECHL
                     PFNFQLILAKWNAAGVRKMVDDYEAALPADGWPNWVLGNHDQHRLATRIGADQARVAN
                     MLLLTLRGTPTCYYGDELGMQDVAIPPEKIQDPPAVNQPEIAHIVGRDPERTPMQWDA
                     SPNAGFASPEVQDLWLPVAPNYKEVNVAKQLEDAYSFLNYFRTLVRYRKSSPALKWGS
                     YASVDPGSMDARENCFVFERRADNNHLLIALNFSAQDQKLSLPGLGSARIALSTKLDH
                     DGEVNLTDFTLRANEGCIIEL"
BASE COUNT         1562 a         1470 c         1544 g         1391 t
ORIGIN      
        1 cagccctgct tatacagccg tgcggatagg acggcaagta aaacaaccgc gacctgcgca
       61 aggatggcag gcaggatctc cgccggaaga actccagcga gaaccagggt gttgattgta
      121 ggtggaagag taaggcttag ggcgagaatc gacaggattg atgcagagac tatcactggc
      181 gcgcggaaca tgttttcaat ttcttgttcc tgctttcgtg gtctgaagaa tacagttctt
      241 tcagcaatca cgtagaagaa tgccagaccc acccatgcca gcgcgttata ggctgatggg
      301 attttgaaca aaatcaacgc ctgaccgaag gcaattgtaa aagtcaaaat actggcagat
      361 gcccagagga acgcaagtct tgatttcctg aaatacgagg cactgaatgt aaacagcgcg
      421 gtgccgacca gcgaaacagc cacaccgatc cacggaatgt aatggttgct ctggctctgc
      481 agcaaggatt gaattattgc ataacccatt agtgcgtagc ctacgctgaa gatcggcaga
      541 ggatgggatt tctcgcggct catttgaaat ttattgagtt ggatggcaac agggatgtat
      601 acaaagcttg caagcagggc ataggcaagg gtttgtacat gatcggatat attgatcatt
      661 tccatggaca actggaacgg ccaaataaat agaacacccg cgatgatggt ttcaatgagt
      721 cgattgtata gataagcaat aacactcgct gcagcaaccg tgattaatag ggcggtagac
      781 aaggcatata tattcggcag ggatatgaaa ataccgagca tgcagaggaa gtatgtcaac
      841 gtgtgaaatg gcagccgata ctccctggcg cgcctaatca gtatttgacc gattccaatg
      901 tacgcaaggc cgactcccgt caataccaca ccgacccgtg gattattgaa atttaagcta
      961 tttccggcca ctgcaaacca aaatggtgtt aacaacgcga ccggccagag aaagatccct
     1021 ttgcccaggc cgtatggcag ccaggtcgaa agaggagaaa gcgaagcatg acgcccactg
     1081 tcttgcaaaa caaacgaaac aagatagacc aggatgcagg cacccaacga cacgagtgtt
     1141 ggaatctttg tccagtttgt tggatcgagc agatagctaa agggcaggca aaaaagagcc
     1201 agaagggtaa ggatatgcgc ccattcgtaa agccatctgt tgtgtttctc aaggccattg
     1261 aaaacagccg ccagaatgat atagaagatt gcgagtgcac cccatgcgac agtgatccag
     1321 gcaattgcag cgggaatttg cagttgcata ccctttagaa gttcaatcgt cgtcatggag
     1381 accggcgcga taaataatcc ggaagccagc aaggtgtatc gagacttcct aaacagatat
     1441 gcacaaagta tcaaatccag cgttgaaaag atcatggcca ggattccacc ccaaacctga
     1501 tttcctacgg acacgataaa tccaccaacg gcgcctatgc tcaaaaggag taatccgctg
     1561 acatttaatg cagccgcata tttctcgatg atcagagggt caatttttag atgtcttgta
     1621 cgctcgctga tcagaaaata agccatagca aggaccgata cggcagccgg ataccattcg
     1681 aaaccaagtc cgccaacgcg cagcgcgaag ataacagttc caatcgatcc gccaatcgcc
     1741 gcgtatgcaa atacaaaggc tgggaactgc cacgccagca ccaggtagcc tgctgttgca
     1801 aacataaaac tcatcatttg gccaacaggt ggcattccag gtgagaagac aatgtaaaac
     1861 aaactggcag ggatcagaat ctgtgaaagg taacgagcgg cacgcgcaaa ttcgcgccat
     1921 tggccattcg cttttgagta ttggcctgcg aacatagtca tgagtgtgcc tgagacggtt
     1981 acagacacaa cactccactc caggtccaat cggagcaggc gggtgaatgt aaataaggtg
     2041 ctagtggcag caagcagggg cagataatcg aagaattcgc ctttcaactt ccatgcggtg
     2101 aatgtataaa gggcggtaca aaaaatagaa acaaacagcc agtatagagg accgttattt
     2161 tgcccgatgc ctcccaactg atagattgct ccaaaatcaa caatcaccag gagcgcgcca
     2221 atgcccgtca aaacggtgcc ggcctgctcg agcttcagcc gaactctcaa ggcccagcct
     2281 cccgcataga aggatagcgg caccgatgca atgaatagga gttggatgat cggatggaat
     2341 tgattccaga accgaatcac taacacagtg gcggatacga cgatcatgaa cgcgccgaga
     2401 tacaataagg cgcgtaatag cgcgccggat gtggcagctt ccaggatcaa cttgcgccag
     2461 tcgatgggcg ggcggggagg cgtgggcggc gcgacaggct tgggaggagc aggtttaggc
     2521 ttgggcgcag caacggcgac tggtatgggt ttcaccacag ctgttggaag tggagctgat
     2581 tccgcttttg cgctcactac aggggctgga atcgcttttt cggttgcagg ttttttctct
     2641 gcaaccggaa ttggcgaggg tttcggttga accggctttt ccttctgaga gactttcgga
     2701 agaagctgcc cgcgcagaag attttgcttt gctcccacga gcgtgagcaa ttgccctctg
     2761 agatcgggtg agatttccgg gcggtcaccc agccagccgg ctaaatattg cagatgctga
     2821 tattcttcca gcagcctgcg ctcaaacgcc tctccacagt gcgaacactg gagcaggacg
     2881 ctgtttccag ttgtgtgttt gcaatttgga cattccatgc tgccacctca ctttgcctga
     2941 cctgttttga ttctaatggt gtgaattgcc ttcactctca ccttcattct tgctcaacct
     3001 gactgccaaa gctagagaat taactgccaa aaactgccaa ctttgcaata aaaaaggacg
     3061 agagaaatct cgtccttttt gcctacactt gtttctgccg atatctatct caaaatcaca
     3121 ccacttcgag ccggaattgt aaacctcagc cgctgatcat tcaccgagag attggaggcc
     3181 tccccataca gcaccttcgg gtttctcgcc tcatacttca cctctgcctg ttgagcggac
     3241 tctgatgcgt tcaaagcaat gataaacgtc tgctgatcga gagagcgaga gaatgcgtac
     3301 acgccatttg cggaccataa gcgcttgaaa tcgccccggc gcagggcagg gtttttcctt
     3361 cgtagtttga tcagatcctt cgcatacttt agcaaatcct tgtcccactt gctttcatcc
     3421 cacggaaatg acttgcggtt gtccggatca tgtttcccgt ctacaccgat ttcgtcgcca
     3481 taataaatgt tgggggcacc agggtaggcg aaaatgaaca tccaggcaag tttcagcgcg
     3541 ttcttatcgc ctccggcaca cgagagaaaa cgcggtgtgt catgcgtatt cagcgaattc
     3601 agttgagaat aggtgacctc gcgcttgtag agctggaggt tgtaatcgat gcgatttgca
     3661 aactcatccg agtcgatcgg gtaatggata ccctgataat tcagctgctg gtgcaaaatc
     3721 ttaagatcga gatgtctgcc cgagaagaac gccagacatg gtcgggcgat atcataattc
     3781 atgatggcat cgaattgatc gccttgcagc cagcgttgcg cctcgtgcca gatctcacca
     3841 actatatagg cttccgggtt gatgtttttc acccgcagac gaaattcctg ccagaacgag
     3901 tcatcatcaa tttcggttgg aacgtccagc cgccagccat ccacgcccag tttgatccag
     3961 tgttcggcga catcaaagag gaattcacgg acgacgggtg atttggtgtt gaatttcggc
     4021 agtgccggca aattccacca gcctcgataa ccaatcgcag taatactgtc ttcgtgctgg
     4081 aggagtcttt gttcgtgcgg cgaaggatag gctccccaat gctttttacg gttcagccgc
     4141 tcagggtcaa acaggaacca atctttatac ggtgaatctt caccacattc gagtacatga
     4201 tggaactgcc agaagccgcg cgaggcgtgg ttgaagacgc catctaaaac aatgcgaata
     4261 ttctttctgt gtgctttttc cagtagttct gtaaaagccc ggttcccgcc taggatggag
     4321 tcgaccgtgt aataatcata ggcatgataa cggtgattag aggcagattc aaaaatcggg
     4381 ttgaggtaga gtgctgtaat ccccaggtct tttaagtaat caagtcgttc aacaacccca
     4441 tacaggtctc cacccttaaa tccgtgtacc gtcggtggag catcccaggg ctcgaaggtc
     4501 aaggtatcgt tggggttgcg tgcacttctg gcaaaacggt cgggaaagat ttgataaaaa
     4561 accgcatcct gtacccaatc tggtatgttc atggtgaaac ctcattgtga gcttacatag
     4621 aatcggacaa gaggtcatct tgtccgatgt tgattctact ttattttgtt gttcctcgtt
     4681 aaaaaggcaa tgatattctt acctgggcaa attgctggct tttactcata actcaatgat
     4741 acaaccctcg ttggcccgta acgtgaagtc ggtgagattg acttcaccgt cgtgatccag
     4801 tttcgtcgag agcgcaatcc tggcactgcc tagcccgggg agactgagct tctgatcttg
     4861 agcagaaaaa ttcaaggcga taagcagatg attgttatcg gcgcgtcttt caaagacaaa
     4921 gcaattttct cgagcgtcca tagagcccgg gtcaaccgaa gcgtaactcc cccacttcaa
     4981 ggctggtgag gattttcgat acctcaccag ggtccggaaa tagttcagaa aagaatatgc
     5041 atcctcgagc tgtttggcga cgttgacttc cttgtagttc ggtgcgacgg gaagccacaa
     5101 atcttgcact tcaggcgatg cgaagccggc gttgggtgaa gcatcccatt gcatgggagt
     5161 tcgttcgggg tcgcgcccga cgatgtgagc gatctccggt tggttgactg ccgggggatc
     5221 ctggattttc tccggtggga ttgccacatc ctgcatgccg agctcatcac cgtaataaca
     5281 ggtgggtgtt ccgcgcagcg ttagcaagag catattagcg acacgtgcct ggtccgcgcc
     5341 aatgcgggtg gctaagcgat gctggtcatg gtttcccagc acccaattgg gccagccatc
     5401 tgcagggagt gccgcttcat agtcatccac cattttgcgg acaccagcgg cattccattt
     5461 cgcgaggatc aactgaaagt tgaaaggcag gtggcattcc aattgatcgg ccgttccata
     5521 atatttcatc agcatatcat tcggcagata ggtttcgccg accatcatac ggtcatcgta
     5581 ggagtcgaga actttgcgca tctcgtgaat cagttcatgc acctccggaa ggttggcggt
     5641 atagatatgg ttaagctgtc caaacggatt gacgccgtcc catgcagaat tgaggggttc
     5701 atcgcggaac tcagtgtcct tcatcatcag cccgatcaca tccacgcgga agccatctac
     5761 gccgcggtcg agccagaagc gcatcacgtc caggatcgca gtgaccacat ccggatttcg
     5821 gtagttcaag tccggttgtt gtttgacgaa ctgatgcaga tagtattgtc cggtagtttg
     5881 atcaaatgtc cacgccggac ctccaaagtg actcagccag ttgttaggcg ggccgccatc
     5941 tggagccgga tcgcgccaga gatacca
//
LOCUS       DASTJP010000754         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_825260, whole genome shotgun sequence.
ACCESSION   DASTJP010000754 DASTJP010000000
VERSION     DASTJP010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825260"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VFQ23_21950"
     CDS             complement(<1..1576)
                     /locus_tag="VFQ23_21950"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HET9909326.1"
                     /translation="MPKVRFDKFYRYDEMTRLLKAYAKEYPNLIRLESIGKSYEGRDV
                     WLITATNFKSGSDGEKPALWVDGNLHASEVTGSAAVLYLIHTLVTRYKEDENITRALD
                     TRTFYIVPRVNPDGAEWALADRPKEIRSVTKPYPYSEDAMDGLTPWEDVDGDGRILQM
                     RVEDSNGGWKAHPDDPRLMIRRDPIETGGKYYRILPEGLLKNYDGVTINVRRPKEALD
                     LNRNFPANWRSESEQFGAGAYPTSESEARNLVNFVINHKNITGVISFHTMSGVLLRGF
                     SVRPDEDQITEDLWTFQTIGAKGTEITGYPHISTHRDFQYHPKQVITGAFDEWSYEHM
                     GVFSWTVEFWSPMKQAGIENIKYIEWYREHPAEDDLKLLKWNDEVLKGSGFLDWHSFD
                     HPQLGKVELGGWDFLHMWTNVPLEFLEKEISPFPEWIVWHALISPKLGLRESSVTSLG
                     GDTYRIRLVVENQGWLPTYVSKKALERNVTRGVFAEIQLPKDASLEAGKAREELGELI
                     GRSYKSAFVNEQNEGTE"
     gene            complement(1707..2165)
                     /locus_tag="VFQ23_21955"
     CDS             complement(1707..2165)
                     /locus_tag="VFQ23_21955"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9909327.1"
                     /translation="MRTRAGVVLIEDNKVALIERHRAGLDYFVFPGGGVDEGESPEQG
                     AIREAMEELGVEVSIKQKIAEIYFGRTSTHVYFLVEGVGGEFGSGTGEEYTDADPNHP
                     EEGIYVPIWMPIEELPKYSNIYPAGLAKLVIQSMKDGWSKEPMVVFEGNP"
     gene            complement(2181..>3019)
                     /locus_tag="VFQ23_21960"
     CDS             complement(2181..>3019)
                     /locus_tag="VFQ23_21960"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9909328.1"
                     /translation="GKKFGFQYATSSDEEIINDPNINTVAILTRHDSHAELAIKALKA
                     GKYVFVEKPLAINSTQLSAVSEQLKANSESLLTVGFNRRFSPLGQQLSAFYKNRNEPM
                     HIHYRVNAGYLPLNHWTHDPEIGGGRIIGEACHFVDFITFLIGASPVSVTAQALPNNS
                     KYRDDNVSMTFTFPDGSIGVVDYLANGDKSMPKERVEVFCGGKIAVLDDFVVLQTIED
                     GKKKELKGTQDKGWVNEWKVFVRTIREGGVPPIPYDQLIAVTKSTFSVIEALKINHVV
                     EI"
BASE COUNT          718 a          815 c          688 g          798 t
ORIGIN      
        1 cctctgtacc ttcgttctgt tcattgacaa acgcgctctt ataggaacga ccaatcaact
       61 caccgagttc ttcgcgggct tttcccgctt ccagtgaggc gtctttcggc aactggatct
      121 cggcgaagac gccgcgagtc acgtttcgct cgagagcttt cttcgagaca tacgttggaa
      181 gccaaccctg attctcaacc acaagccgga tgcgataggt atcccctcca agtgaggtaa
      241 cgctgctttc gcgcaaccca agtttcggcg agatcaacgc atgccacaca atccactccg
      301 ggaacggact gatttctttt tccaaaaatt cgagcggaac gttcgtccac atgtgcagga
      361 agtcccagcc acccagctcg acctttccaa gctgcgggtg atcgaacgaa tgccagtcca
      421 gaaacccact gcccttcagt acttcgtcat tccacttgag aagtttgaga tcatcttcag
      481 ccgggtgctc gcgataccac tcaatatatt tgatgttctc gataccggcc tgtttcatcg
      541 gactccaaaa ctcaaccgtc catgaaaaca cccccatatg ctcataggac cattcgtcaa
      601 atgcgcctgt gatcacctgc ttgggatgat attggaagtc gcgatgggtg gagatgtgtg
      661 gataccccgt gatctcggtc cccttcgcgc cgatcgtttg gaacgtccat aaatcttcgg
      721 tgatctgatc ctcatccggt ctcacgctaa agccgcgcag taagactccg ctcatggtat
      781 ggaacgaaat gacaccagtg atattcttgt gattgatgac gaagttgacg agattacgcg
      841 cttcggattc actggtcggg taggcccctg caccaaactg ttcagactcc gagcgccagt
      901 tggccgggaa gttgcgattc aagtcgagcg cttcctttgg gcggcggaca ttgatcgtca
      961 cgccatcgta gttcttcaac aaaccctctg gcaaaatccg gtaatacttt ccaccggtct
     1021 cgattggatc gcgccggatc attaaacgcg ggtcgtcggg atgagctttc cacccaccat
     1081 tggagtcttc gactcgcatc tgcaagattc gcccatcgcc gtcaacgtcc tcccaggggg
     1141 tcaatccatc catggcatct tccgagtatg gatatggctt ggtcactgag cgaatctctt
     1201 ttggacgatc tgccagcgcc cattccgcgc catccgggtt gacgcgcggg actatataaa
     1261 acgtccgcgt atctaatgcg cgcgtgatgt tttcatcctc tttatatcgg gtgaccagag
     1321 tatggatgag atagagcaca gcggcggagc ctgtcacttc ggaggcatgc aggttgccat
     1381 ccacccataa tgccggtttc tccccgtcac tcccggactt gaaatttgtc gccgttatca
     1441 gccagacatc tcgtccctca tagctcttgc cgatagactc caaccggatc agattcgggt
     1501 attcctttgc ataagctttc aacagtcgag tcatttcatc gtagcgatag aatttgtcaa
     1561 aacggacctt gggcatggga atcctccgtg agatggaatc aaaacggcac agagatacgt
     1621 tgaaagactg catctccgtg ccgcgttgca agtttcatga ttctatttta ccgcctgtgg
     1681 tcgtgactgc tcagcacgtg cgccttttat ggattgcctt caaaaacgac cattggttct
     1741 tttgaccacc catctttcat agactgtatc accaatttcg ccaggcctgc cggataaata
     1801 ttactatatt tcggtagctc ttcgatgggc atccagatag gaacgtatat tccttcttcc
     1861 gggtgattcg ggtctgcatc ggtatattct tcgccagtgc ctgagccgaa ctcaccacca
     1921 acgccttcga ccaaaaaata gacatgcgta cttgttcgcc caaagtagat ttcagcgatc
     1981 ttctgtttta ttgaaacttc aacacccagt tcctccatcg cttcacggat cgcgccttgc
     2041 tcgggcgatt cgccttcgtc cacgccaccg ccagggaaaa cgaaataatc caacccggcg
     2101 cggtgacgtt cgatcagcgc aactttgtta tcttcgatga gtacaacgcc cgcacgagtt
     2161 cgcatgagat ttctacgaca ttatatttca actacatggt tgattttcaa cgcctcaata
     2221 actgagaatg tagacttcgt cacagcgatc aattgatcat acggaattgg cggaacgcca
     2281 ccttcacgaa ttgtccgtac aaaaactttc cattcgttga cccagccttt atcttgagtt
     2341 cctttcagtt ctttcttctt tccgtcttct atcgtttgga gcaccacaaa atcatccagc
     2401 accgcgatct tgccgccgca aaagacttcg actctttctt tcggcatgga cttgtctcca
     2461 ttggcaagat aatccacgac acctatagaa ccatcaggga aagtaaaggt catcgagaca
     2521 ttgtcgtctc gatatttgct gttatttggt aatgcctgcg cggtcacaga aactggagac
     2581 gcgccgataa ggaaagtaat gaaatctaca aagtgacagg cttcgccgat gatccgtccc
     2641 ccgccgattt ccgggtcatg agtccagtga tttagaggaa gatagcccgc atttactcga
     2701 taatgaatat gcatcggctc gttacgattc ttatagaaag cagaaagttg ttgaccaaga
     2761 ggagagaaac gacggttgaa accgacagtg agcagtgact cgctattggc ttttaactgt
     2821 tcgctgaccg ctgaaagctg agtgctattt atagccaatg gtttctcaac aaaaacgtac
     2881 ttcccggctt tcaacgcttt gattgcaagt tcagcgtgtg agtcgtgacg tgtgagaata
     2941 gcaacagtat tgatgtttgg atcgttgata atttcttcat cagacgaagt tgcatattga
     3001 aaaccaaatt tcttgcctg
//
LOCUS       DASTJP010000755         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_122901, whole genome shotgun sequence.
ACCESSION   DASTJP010000755 DASTJP010000000
VERSION     DASTJP010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122901"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFQ23_21965"
     CDS             <1..348
                     /locus_tag="VFQ23_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9909329.1"
                     /translation="ASGMVPAEGFKHSGANSPESLLLGRKITEVPELVRAANPETYIH
                     PNVPPFFIQHGTSDDTVPHQQSVNFAQRVREVCGEERVIHELLEGARHGDPAFETLEN
                     VNKVLDFLDKNLK"
     gene            425..1141
                     /locus_tag="VFQ23_21970"
     CDS             425..1141
                     /locus_tag="VFQ23_21970"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET9909330.1"
                     /translation="MSEKRLLVIGAHSADFVWRSAGTIAVITSQGGSANVVALSYGER
                     GESGELWKEANQTVDNVKRIRHEEASAAAEYVGATFRCLDLGDYPLSMADTATEQLVG
                     IIQDFKPTILLTHSPADPFNPDHPTASQIAQKARLLSTGAGVASAFSRIMPPDLYFFE
                     PHQPELCDFKPNTFVDITAVMDKKTKAMDSMKSQSYLHKYYSELASRRANHARRISGS
                     DSIKFAEAHFRTLPWVVNSL"
     gene            1138..1770
                     /locus_tag="VFQ23_21975"
     CDS             1138..1770
                     /locus_tag="VFQ23_21975"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HET9909331.1"
                     /translation="MNTNDMMRLCELGVATVYEASGREGLIDIPLIQLLPNSRVAGPA
                     RTVLCAQNDNLMVHAVIEQIQPGEVLVLSMPEPAPVALIGELLATQVKVRGAAGILVD
                     AAVRDVAELIDMGLPIWTRFIRVKGADKKKIGEVNAPVVVGGTAITTGDILILDTDGA
                     VCVKRERADEVLKASEARLLKEGNLRSRLLAGETSYDLHGLRAYVEGGGK"
     gene            1767..2720
                     /locus_tag="VFQ23_21980"
     CDS             1767..2720
                     /locus_tag="VFQ23_21980"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HET9909332.1"
                     /translation="MTDEMIHDIAHLGHVELLTPEPEESLRFFVDVMGMTETAREGDS
                     VYLRGWDDYEHHSLQLTASAKPRLGHYALRAASPQALGRRVDAIEKSELGKGWKENNL
                     GHGPAYCFCDPDGHPIEIYYETEWHQPSAETKPALKNQASRYSDRGVGVRRLDHIALF
                     AADVRSNRQFMQNILGARMTEQVIFDDGSEKAAWVTFTNKSYDVTFAEDAYGAKGRLH
                     HFTYAVDNREAILRAADICLEHGVFIETGPHKHAIQQTFFLYVYEPGGNRFEIANAGA
                     RLILAPDWRPIVWSEAERKKGQAWGLKTIESFHTHGTPPVK"
     gene            2731..>2967
                     /locus_tag="VFQ23_21985"
     CDS             2731..>2967
                     /locus_tag="VFQ23_21985"
                     /inference="COORDINATES: protein motif:HMM:NF014958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HET9909333.1"
                     /translation="MPHLTLEYTDNLDVDVQPLLARLHEEIVATGAINLKGIKSRVIC
                     HTQYRIADGNPDYAFVHVELLIREGRPLDIQKEAT"
BASE COUNT          786 a          716 c          804 g          661 t
ORIGIN      
        1 gcatcaggaa tggtgcctgc agaaggcttt aagcacagcg gcgccaactc acccgaatct
       61 ttgcttctcg gaaggaagat caccgaagtt cctgaattgg tccgtgcagc aaatccagaa
      121 acatacatcc acccaaatgt accgccgttc tttattcaac atggcacaag cgacgatacc
      181 gtaccccatc agcaatctgt taattttgct caaagagtca gggaagtttg cggcgaagag
      241 cgagtcatcc atgaactgct cgaaggggca agacacggtg atcctgcatt tgaaactctc
      301 gaaaacgtaa ataaggtcct ggactttctg gataaaaact taaagtaaca aacagaggtg
      361 acagttggat tggagccggc tgtcacctgt attcagattt acaaagagcc aaaggaaacg
      421 atctatgagc gagaagagat tactcgtgat tggagcgcac tctgccgatt ttgtctggcg
      481 ctcggcaggg acgattgctg tgatcacatc acaaggtggc agcgcgaatg ttgtcgcact
      541 ctcgtatggc gaaagagggg aatcgggcga gctttggaaa gaggcgaatc aaacggtcga
      601 caatgtgaag cgcattcgtc atgaagaggc aagcgcggca gcagaatacg tcggtgcaac
      661 gttccgctgc cttgaccttg gcgattatcc attaagcatg gcagatacag ccacggagca
      721 gttggtggga atcattcagg atttcaagcc gactattttg ctcacacaca gccctgctga
      781 cccattcaac ccggatcatc cgaccgccag ccagatcgcg caaaaggcac ggctgttgtc
      841 gacaggagca ggcgttgcca gtgcgttcag ccgcatcatg cctcccgatc tgtatttctt
      901 cgaaccacac cagccagaat tatgtgattt caaacccaac acgtttgttg atatcacagc
      961 cgtgatggac aaaaagacaa aagcgatgga ttcaatgaag tcgcaaagct acctgcataa
     1021 gtactacagc gaacttgcct cccgtcgtgc gaatcatgca cgccggatct ctggcagtga
     1081 cagcatcaag tttgcggaag cacattttcg aacgctgccc tgggtagtaa acagcctatg
     1141 aacacaaatg acatgatgcg cctctgtgag cttggcgtcg caacggttta tgaagcatcc
     1201 gggcgtgaag gattgatcga tattcccctg attcaactcc ttccaaatag ccgcgtggcg
     1261 ggtccggcgc gcaccgtctt atgcgcacaa aacgacaacc tgatggtgca tgccgttatt
     1321 gagcaaatcc agcccggaga agtattggtc ttgagcatgc cggaaccggc gcctgtggca
     1381 ttgatcggtg agttgctcgc tacacaggtc aaggtaaggg gagcggcagg gattctagtc
     1441 gatgcagcag ttcgagatgt ggcggagttg atcgacatgg gtctgcctat ctggacacgt
     1501 ttcattcgtg taaaaggtgc agataagaag aagatcgggg aagtgaacgc gcctgttgta
     1561 gtgggcggaa cagctatcac aacaggtgac attctgatcc tggatactga tggcgctgtg
     1621 tgtgtcaagc gtgaacgcgc cgacgaagtg ctcaaagcat cagaagcgcg gcttttaaaa
     1681 gaaggaaatc tgcgcagtag attattggca ggagaaactt cttatgacct tcatggtctg
     1741 cgagcatatg tggaaggagg cgggaaatga ccgacgagat gattcatgat atcgcccatc
     1801 ttggtcacgt ggaactgctg actcccgagc ctgaagaaag tctgcgcttt tttgttgatg
     1861 taatgggtat gacggaaact gccagggaag gcgattccgt ttatctgcgc ggctgggatg
     1921 actacgagca tcactcgctc caactgaccg cttctgccaa accaaggcta ggacattatg
     1981 cattgcgtgc cgcaagtcca caggcattgg gacggcgcgt ggatgcgatc gagaaatcgg
     2041 aattaggcaa aggttggaaa gagaacaatc ttggtcacgg tcctgcctat tgtttctgcg
     2101 acccggatgg acatcccatt gagatctatt acgagaccga atggcaccaa ccatcagcgg
     2161 agacaaagcc ggcgttgaag aatcaggcgt cccgttattc agatcgaggc gtgggtgtgc
     2221 ggcggctgga tcacatcgct ttattcgcag cggatgttcg gtccaaccgt caattcatgc
     2281 agaacattct tggcgcgcgc atgactgagc aggtcatatt cgatgatggt tctgaaaagg
     2341 cagcctgggt cacgttcacg aacaaatcct atgatgtgac ctttgccgaa gatgcgtatg
     2401 gagcaaaagg acgtttacat catttcacat acgcagtgga taaccgcgaa gccatcctgc
     2461 gcgcggctga tatctgtttg gaacatggcg tctttatcga aacgggtccg cacaaacacg
     2521 ccattcagca gacgttcttt ttgtatgttt acgaaccggg tggaaatcgt tttgagattg
     2581 ccaatgccgg ggcacgtctc atccttgcgc cagattggag acctattgtc tggagcgaag
     2641 ccgagcgcaa aaaaggtcag gcatggggac tgaaaacgat cgagtcattt cacacgcacg
     2701 gcacaccgcc tgtcaaataa ggagaaacac atgccacatc ttacgctgga gtataccgac
     2761 aaccttgacg ttgacgttca accgcttctg gcacgtttac atgaagagat cgttgcaaca
     2821 ggcgcgatca acctgaaagg aatcaagagc cgtgttattt gtcacacaca ataccgcatc
     2881 gcggatggaa acccggacta tgcatttgtc catgttgaac ttttaatccg cgaaggacgt
     2941 ccactggata ttcagaagga agcgaca
//
LOCUS       DASTJP010000756         7767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_68499, whole genome shotgun sequence.
ACCESSION   DASTJP010000756 DASTJP010000000
VERSION     DASTJP010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68499"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="VFQ23_21990"
     CDS             complement(<1..1186)
                     /locus_tag="VFQ23_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909334.1"
                     /translation="MKKVSTSEYKKRLAAWKKAEKEQAETQKVVWNRWIEDLDANKAL
                     SPIEKAEAWLHQIMIKGKSIIDSGDWNAQLTVDEFVDQEALIYFVSRGVDCREQIKNM
                     TSRLKEQKKKSEAFVKTVESWSDKELRFMGFRKKKKNPRKSNEIPSPEEAMWRNTIPL
                     TDDNQPHNLVSTYRIIEWCDIGGFDEMWESHAERLREDVARGGEIKSELACQFLFNIC
                     RSDFAIEIFEEDLLKLLSIVEAPDYQLTIPWHRWNRDDMSLLAGVKSNSIFAYAASIA
                     FSNGRIRKEKTNKEVVNQALKWLLESQEKNGSWKFSTVLDEPSILATCMVVHALGLSR
                     PRGWKSSAALACEWLLSKQDSFGFWFESTSLDIDPVYLTVLVLDTLELAAESPKLTFH
                     VNK"
     gene            complement(1392..2153)
                     /locus_tag="VFQ23_21995"
     CDS             complement(1392..2153)
                     /locus_tag="VFQ23_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909335.1"
                     /translation="MISAFDYKDNCWWCGNKADSGEHKYKKTDLVREFGTGPYKGSNS
                     IVRVLNGKQRIIQGPDSQEAKFESNLCRTCNNAKSQPFDYEYDTFTTFIKEEEDRIYT
                     TRQFKLSDIFGSNWMPKKQNLVRYFVKHICCRVAASNILIEPEIIEFLNGGSVSQYLS
                     LWFEIREDIVAFIKDSKENGEDLGILWLGEMTYQKSRGTGKISEITSYIGYRWLRLNY
                     KYDKSISPSRDNFSSEVVQLESNYNVDPGTIPKIS"
     gene            2384..2845
                     /locus_tag="VFQ23_22000"
     CDS             2384..2845
                     /locus_tag="VFQ23_22000"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9909336.1"
                     /translation="MIPRTAVFLRRGDSYLLLKGAPTKRLWANLYNGLGGHVEQGEDV
                     LSAAKRELLEETGLTADLWLCGTAIVNTGDAQAGICLFFFTGERTDGEPRPTEEGTAE
                     WIHHDALAEIPVVEDLPVLLAKIHHMQRGDPPFSARSHYDEAGKLIVEFGA"
     gene            complement(2908..3699)
                     /locus_tag="VFQ23_22005"
     CDS             complement(2908..3699)
                     /locus_tag="VFQ23_22005"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909337.1"
                     /translation="MKVNRKKKLGSLLLILLVVSACAAPANNQPGVTETASAQSTSTE
                     LSSSTELCSNRYFPVREGATWTYKNSGSPAGEFQFTDRVSSVRADGFTLTSQLGNTAW
                     TQEWACQQEGLVALQLGGAPAMTLTAQGVQLNLAVNNVSGVTFPRDIIAGNQWQHNLD
                     FDGQVEMAGQSGNAQGTALTRFTVLGNETVTVPAGTFEAVKIQVEPTLDINVGFQLLS
                     IPVTISGTYTYWFVQDVGWVKASGAGNFAGQTLSETIELQSYHVP"
     gene            complement(3716..4885)
                     /locus_tag="VFQ23_22010"
     CDS             complement(3716..4885)
                     /locus_tag="VFQ23_22010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909338.1"
                     /translation="MTTQRAPSKIIPRAFPGIKLPEVEELIANSQVRTYSAGTVLCRE
                     NAVEDRFYMILEGEVEVTKNINNNETRLLKTLSPGDFFGEMALIHNAPRAATVVAKTE
                     LTTLELDKNGFDRVLHNSTSIAMAMVSEISNRLRANDQMAVDDIRIRATELANAYQKL
                     AEQEMARREFLSNIAHELRTPLMIATGFLQMLQRGMVSGELLKNGIDTVARNVQQIAT
                     LVNDILFLQEMELVLPEFQPVDMSELASQVLERYKAKALERGVRLRLEADSNLPMASG
                     DPKSLERALMAIVDNAIKFSPMGGDVIVRLRSTNENVIVAVTDHGIGMSSDTLPRIFD
                     RFYHTERQDDNLFGGIGLGLSITRQVIMQHQGSLDVTSEPNQGSTFTLTLKKWRV"
     gene            complement(5026..5514)
                     /locus_tag="VFQ23_22015"
     CDS             complement(5026..5514)
                     /locus_tag="VFQ23_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HET9909339.1"
                     /translation="MEIQTVKIEKPEVINFILGQSHFIKTVEDIHEALVSAVPGIKFG
                     LAFCEASGKCLIRWTGTDPEMIGLAKQNAEAVGAGHSFILFLGEGFFPINVLNSIKNV
                     PEVARIFCATANPTEVLVAQTEQGRGILGVVDGFSPQGVEDEEGIAWRKDFLRMIGYK
                     AT"
     gene            complement(5630..6772)
                     /locus_tag="VFQ23_22020"
     CDS             complement(5630..6772)
                     /locus_tag="VFQ23_22020"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HET9909340.1"
                     /translation="MYKRKIQINIVLFIAALVTQVTTVFAAPALQPQKGVRLAPARAA
                     APGWIVSCQYSHSLPDDPIVAFRQPGASHMHDFVGARVTDAFSTATALVAGGTTCAMP
                     GDKSAYWVPALFKNGVRVLPQGTSKHALFYYRRKGAPTGVNVQPFPLGLRMIIGNARA
                     TSPAQNPQLGRDIIFKCGPGSTTDLPAPPTQCSSGILVMSLRFPNCWDGRNLDSADHK
                     SHMAYPAGSRCPASHPVVLPRIESFFRYNVGTGPIGTITLASGPYYTAHQDFFNAWDP
                     AVLQRLVTSCMNAMRDCGTNPTVSASTTNSLLAASTLAATSTALPTVTPALISTNTPL
                     PLPTNTVAPTFTAIPPTDVPTEAPTEVPTEVPTEAPPTAEPTQPSP"
     gene            complement(7095..>7767)
                     /gene="nfi"
                     /locus_tag="VFQ23_22025"
     CDS             complement(7095..>7767)
                     /gene="nfi"
                     /locus_tag="VFQ23_22025"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HET9909341.1"
                     /translation="GLSPEKAIRVQAALRGRLILSWDHRPVKTIGGVDISIKTDIART
                     AIVAISYPDLTPLESVVADAPLVFPYIPGLLAFREGPAMLAAWDKLQAKPDMLMFDGQ
                     GIAHPRGVGIASQMGLWLKRPTIGVAKSRLYGRHEEVGPKRGHQAALIDKNGIFIGAA
                     LRSREKTNPLYVSPGHWIDVNHSTEYIMACCTTYRLPEPTRWAHKVASGDALPNEDEQ
                     QPRLF"
BASE COUNT         1841 a         1967 c         1858 g         2101 t
ORIGIN      
        1 ctttgtttac gtgaaaagta agttttggac tttcggctgc aagttctaat gtgtctagaa
       61 ccaagactgt taaataaacg ggatctatat caagagaggt cgattcaaac cagaacccaa
      121 aactgtcttg cttagaaagt aaccattcac atgcgagagc agcagaactt ttccaccctc
      181 ttggcctgct aaggccaagg gcatgaacca ccatacaagt ggctaatatt gatggttcat
      241 ctaggaccgt cgaaaacttc caagaaccat tcttttcttg actttctagt aaccacttga
      301 gtgcttgatt aactacctct ttgttggttt tctcttttcg gattctcccg ttggagaaag
      361 cgatactagc cgcgtaagca aagatagaat tcgactttac gcctgctaac aaactcatat
      421 cgtcgcgatt ccagcgatgc cacggaattg tcaattgata atctggcgct tctacaatgc
      481 ttaatagttt cagcaaatct tcttcaaata tttcaattgc aaaatcagag cgacaaatat
      541 tgaataagaa ttgacacgct aactctgact tgatttctcc tccccttgcg acatcttctc
      601 ttagtcgttc agcgtgactt tcccacattt catcaaagcc acctatatcg caccattcaa
      661 tgattctgta cgttgataca agattgtggg gttgattatc gtcggtaaga ggaatagtgt
      721 tcctccacat agcttcttca ggggaaggta tctcgtttga tttccttggg ttcttctttt
      781 tcttgcgaaa ccccataaat ctgagttctt tatctgacca agactcgacg gttttgacaa
      841 aagcttctga tttcttcttt tgttctttca gtcttgaggt catgttcttg atttgttcgc
      901 gacaatcaac ccctctgctc acaaagtata tgagagcctc ttgatctaca aactcatcta
      961 cggtaagttg tgcattccag tcacccgaat caatgataga ttttccctta atcatgattt
     1021 ggtgtagcca ggcttcagcc ttttctattg gcgaaagtgc cttatttgcg tcgagatcct
     1081 cgatccagcg attccagacg actttttgtg tttcggcctg ttccttctca gcttttttcc
     1141 aagcggccaa gcgtttctta tactctgatg tcgatacttt tttcatatat aaatattata
     1201 cggcatccgt ctatatggaa aatgctactt tgattatgga cgggaaacgc ccaacggctg
     1261 gcgttagccg cttggggcgg gagcgtggac tcgccgtcga aacggaaacc cctcaaagct
     1321 caaagaaatt ctgaaaaacc gcggtgtcta cctatctgtc aattttccca tgacgttggg
     1381 tgctttctgc ctcatgatat cttaggtatc gtgccaggat ccacattgta gtttgattca
     1441 agctgaacta cttcagagga gaagttatct cgggaaggag aaatactctt gtcatactta
     1501 tagttaagcc tcaaccaacg atatccaata taacttgtta tttcacttat tttgcccgta
     1561 cctcgacttt tctggtatgt catttccccg agccaaagaa ttcctagatc ctctccattc
     1621 tccttagaat ccttgataaa agctactatg tcctcacgaa tctcaaacca tagcgataaa
     1681 tattgagaga cggagccacc attgagaaac tcaatgattt caggctcaat tagtatattg
     1741 ctggcagcca ccctacaaca aatgtgcttg acgaagtaac gaaccagatt ttgttttttg
     1801 ggcatccagt ttgaaccgaa tatgtctgag agtttgaatt ggcgcgttgt ataaattcta
     1861 tcttcttcct ctttgataaa agttgtgaag gtgtcatatt cataatcaaa tggttggctt
     1921 tttgcgttat tgcaagttcg gcataaattg ctttcgaact ttgcctcttg cgaatccgga
     1981 ccctgaataa tccgctgttt gccattcaga accctcacaa tagaattgct tcccttgtat
     2041 ggccctgtgc caaactctct cactaaatca gtttttttat atttgtgttc tccagagtca
     2101 gctttattcc cacaccacca acagttatct ttgtaatcaa aagctgaaat cattcattcc
     2161 accatctgag agattctact ctagcgcgag cgcccaacta gtgtttatat gacaaaaatc
     2221 tgcaaaacgg cttatagaca gctcgttgct gttagtatct taatttgttt agcctaagag
     2281 tttaggctgc gttacgataa aatactaaaa aattatagca tatgcagaaa gccaaattga
     2341 gatgccacaa tccaatcaag gtgttacaaa ccatcgatac accgtgatac cacgcaccgc
     2401 cgtctttctt cgtcgtggtg attcatacct gctcctcaag ggcgcaccga cgaaacgcct
     2461 ttgggcaaat ctctataacg gactgggcgg tcatgtggag caaggagaag acgtcctctc
     2521 tgccgccaaa cgcgaactgc tcgaagagac cggcttaacg gcagatctat ggctgtgcgg
     2581 cacggcgatt gttaacacgg gcgacgccca ggcgggaatt tgtctgtttt tcttcaccgg
     2641 tgagcgtacc gatggagaac cccgcccaac ggaagaagga actgctgagt ggattcacca
     2701 cgatgcgctt gcggaaatcc cggtcgtaga agacttgccg gttctcctgg cgaaaatcca
     2761 tcacatgcag cgcggcgacc ctcccttcag cgcgcgctcc cattatgatg aagcaggaaa
     2821 gttgattgtg gagtttggcg cataaataga aaacccctct cctgctggga gaggggatag
     2881 gggtgagggc ggaatcaata ctactatcta cggaacgtga tacgactgca attcgatcgt
     2941 ctccgaaagg gtttgaccgg caaaattacc cgcaccgctt gctttcaccc agccgacatc
     3001 ctgcacaaac cagtaggtgt aggtgccgct gatggtcacc gggatggata gcagttgaaa
     3061 tcccacattg atatcgagag tcggctctac ctggattttc accgcctcga acgtcccggc
     3121 gggtaccgtc actgtttcat tccctagtac ggtaaaacgc gtcagagccg taccttgcgc
     3181 attgccggac tgacctgcca tctccacctg accgtcaaag tccaggttat gctgccactg
     3241 gtttccagct atgatatccc ttggaaaggt cacaccgctc acattattaa ctgccagatt
     3301 gagctgtacg ccctgagcgg tcaacgtcat cgcgggtgca cctccgagtt gtaacgccac
     3361 cagaccttcc tgctggcatg cccattcctg cgtccaggcc gtattaccaa gctgcgatgt
     3421 gagtgtaaat ccatcggcgc gcaccgagct aacgcgatct gtaaattgaa attctccagc
     3481 cgggctgccg gagttcttat aggtccaggt cgcgccttca cggacgggga agtagcggtt
     3541 gctacagagt tcggtgctcg atgataattc tgttgatgtg ctttgtgcgg atgcagtttc
     3601 tgtaaccccc ggttggttgt tggctggtgc tgcgcatgcc gaaacgacaa gcaggatcag
     3661 cagcaggcta cctaattttt tctttcgatt caccttcatt gaaaatagtc acctttcaca
     3721 ctctccattt ctttagggtt aaggtaaatg tacttccttg gttgggctca ctggtcacgt
     3781 ccaggctgcc ttgatgctgc atgatcacct ggcgtgtaat cgacaaaccc aggccgatcc
     3841 cgccgaagag attatcatcc tgtcgttccg tgtgatagaa gcgatcaaaa atacgcggca
     3901 gggtatcgct ggacatccca atcccgtggt cggttacggc aacgatcacg ttctcgttgg
     3961 ttgaccgtaa tcgcacgatg acgtcgccgc ccatcgggct gaacttaatc gcattgtcga
     4021 caattgccat gagggcacgt tcgagcgatt ttgggtcgcc agacgccatg ggcaaattgg
     4081 agtcggcttc gaggcgcaat ctcacaccgc gctccagcgc tttcgctttg tagcgctcaa
     4141 ggacctgact cgcgagttcg ctcatatcca ccggctggaa ctcgggcaac accaactcca
     4201 tttcctgcag gaagaggatg tcgttgacca gcgtggcgat ttgctgcacg ttacgcgcca
     4261 ccgtatcgat gccgtttttc agaagttcgc cgctcaccat gccacgctgc agcatctgga
     4321 gaaacccggt tgcgatcatc agcggcgtgc ggagttcatg ggcaatgtta ctaaggaact
     4381 cacgccgcgc catttcctgc tctgccagtt tttgataagc attcgccagt tcggtcgccc
     4441 ggatgcgaat atcgtccacc gccatttgat cgttggcccg caggcgattg ctgatctcgc
     4501 tgaccatcgc catcgcaata ctggtactgt tatggagcac acggtcgaac ccgttcttat
     4561 ccagttcgag cgtggtcaat tctgttttag caacgacggt ggcggcccgc ggcgcattgt
     4621 ggatcaatgc catctctccg aaaaaatcac cggggctaag cgtcttgagc aggcgcgtct
     4681 cgttgttgtt gatgttcttt gttacttcca cttcgccttc gaggatcata taaaagcggt
     4741 cttcgacagc gttctcgcgg caaagaacgg ttcccgccga gtatgtgcgc acctgactgt
     4801 tggcaatcaa ctcttcgacc tcgggcagtt tgatgccagg aaaagcacgt ggaatgatct
     4861 tggaaggggc tcgttgggtg gtcattctgt ctgtgggtta gattgggtcc tggcgcaatt
     4921 ttagcacgcc cagggcagct gtaactttac attttcatac taatagggaa ctgcaaggaa
     4981 acaactgtaa ctcttaccag agcctctttg gagtatagcc ctctcctatg tagccttata
     5041 tccgatcatt ctcaaaaaat ctttgcgcca ggcgatgcct tcttcatcct ccacgccctg
     5101 cggcgaaaac ccatcgacca cgcccaggat gccgcgtccc tgctcggtct gcgcgaccag
     5161 cacttcggtc gggttggctg tggcacagaa gatacgcgcc acttccggca cattcttaat
     5221 ggagttcaga acgttgatgg ggaagaagcc ctcgcccaaa aagaggatga atgaatgtcc
     5281 cgcgccaact gcctccgcgt tttgcttcgc caatccgatc atctctggat ctgtgccggt
     5341 ccaccggatg agacacttcc ccgaagcctc acaaaaagcc aatccaaact tgattccagg
     5401 caccgcactt accaatgctt cgtgaatatc ctccaccgtc ttgatgaaat gtgattgacc
     5461 caggatgaaa ttgataactt caggtttctc gatttttacc gtttggattt ccatggagtc
     5521 tccttttttg gttggcacat ttcaagattc aattatgttc attatacaaa acaatcccgc
     5581 ccacaatggg gcgggattgc cttcgttgga attcttttca gagcgcttat tacggtgaag
     5641 gttgtgttgg ctcagccgtc ggtggtgcct cagtcggaac ttcggtcgga acctcggttg
     5701 gagcttccgt cggaacatcc gttggcggta ttgcagtgaa tgtcggtgcc acggtattcg
     5761 tcggcaaagg caacggtgta ttcgtggaga tgagagccgg agttaccgtt ggcaatgcgg
     5821 tgctcgttgc ggcgagtgtg ctcgcagcca gcagtgagtt cgtggttgat gcgctcacag
     5881 tgggattcgt accacaatcc cgcatggcat tcatacaact cgtgacaagt ctctgcagca
     5941 cggccgggtc ccaggcattg aagaagtcct gatgtgcggt gtaatagggt cccgacgcga
     6001 gcgttatagt gccaattgga cctgtcccaa cgttgtaccg gaagaacgac tcgattctcg
     6061 gaagtacaac gggatgcgat gccgggcagc ggcttccagc cggatacgcc atatgggact
     6121 tatgatcggc actatccagg tttctgccat cccaacagtt ggggaaccgt aaggacatga
     6181 ccaggattcc cgaggagcat tgggtgggcg gtgcaggcag atcggtggtg gatcccggtc
     6241 cgcatttgaa gatgatgtct ctgcccaatt gagggttttg agccggggat gtggctcgtg
     6301 cattgccgat gatcatcctc agtccgaggg ggaacggttg tacattcact cctgtgggcg
     6361 cgcccttcct gcgataatag aacaacgcat gtttcgatgt tccctgaggc agaacgcgga
     6421 ctccattttt gaagagtgcc ggtacccaat atgcagattt atcgccgggc attgcgcagg
     6481 tagttcctcc ggctacaagt gccgttgcag ttgaaaacgc atcggtcact cgtgctccaa
     6541 cgaaatcgtg catgtgagat gcacccggct gacggaacgc cacgatggga tcgtcaggaa
     6601 gggaatgtga atattgacaa gacacaatcc acccgggtgc tgcggctcgc gcgggagcca
     6661 gcctgactcc tttttgcggc tgcagtgcag gtgctgcaaa gacagttgtg acttgagtca
     6721 ccagtgcagc gatgaacagt acgatattga tttggatctt acgtttatac attgtcggtg
     6781 cctcctaccg gtttgatgtt caaattctac agacggcagc gagagctttc cagcaatatt
     6841 gcgataaatc aatttcaata gtgcaagtcg ggctagtacc gcggtatagg acctcattaa
     6901 aaattcattg catgtagagt aatttttctc ataaaggtta catcaaaaca ccttgctcat
     6961 ttgaaagcca aaaataatca gcaaagattc tgtttgtgta agtgtttcat gcgttcgatc
     7021 tttaataact aaattgagtt tgacctgatc catatatcca cccgtcagtt catttgcctt
     7081 cgaagtttga tgttctaaaa tagacgcggt tgttgctcgt cttcattagg taatgcatcg
     7141 ccactggcaa ctttgtgtgc ccagcgagtc ggttctggaa gacgatatgt tgtacagcaa
     7201 gccattatat attccgttga atgattcaca tcgatccaat gtcctggaga aacataaaga
     7261 ggatttgttt tctcgcgcga acgaagagct gctccaatga aaattccatt tttatcgatt
     7321 aatgctgctt gatggccgcg ctttggaccc acttcttcat gtcgtccata caaccttgat
     7381 ttggcaactc cgatcgttgg acgtttcagc catagtccca tttgggaagc gattccgacg
     7441 ccgcgcggat gagcgatgcc ctgtccgtca aacatgagca tatctggctt ggcttgtaat
     7501 ttgtcccagg ctgccagcat agccggaccc tcacgaaatg ccagcaaacc agggatgtat
     7561 ggaaatacta agggtgcgtc ggcgaccact gactcgagcg gcgttagatc tgggtaactg
     7621 atagccacaa tcgccgtgcg cgcaatatct gtttttatgc tgatatccac gccgccgata
     7681 gttttgacag gccggtgatc ccagctcaga atcaaccgcc ctcgcaacgc tgcctgaacg
     7741 cgaatagcct tttcagggga gagaccc
//
LOCUS       DASTJP010000757         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1468684, whole genome shotgun sequence.
ACCESSION   DASTJP010000757 DASTJP010000000
VERSION     DASTJP010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468684"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFQ23_22030"
     CDS             complement(<1..193)
                     /locus_tag="VFQ23_22030"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HET9909342.1"
                     /translation="MKSYRKELWFNIPSRRGFVNITPDVEVALRESGVKEGFVLVNAM
                     HISASVFINDDESGLHHDYD"
     gene            complement(200..775)
                     /locus_tag="VFQ23_22035"
     CDS             complement(200..775)
                     /locus_tag="VFQ23_22035"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HET9909343.1"
                     /translation="MDIFTAIFLLALVVEMVIRAPLNKKRKQEKMSERRVTDQEKIIL
                     GLLSLGGFFIPLIYAFTNWLDFANYELPAWAGWLGVVLLTGALYVFWRAHADLGLNWS
                     PTLEIREQHELITRGIYSVIRHPMYASQWLLAIAQPLLLQNWIAGFLNLLLFIPFYLF
                     RVRAEEQLMIKQFGDQYRSYMQKVGRVLPKV"
     gene            1031..1996
                     /locus_tag="VFQ23_22040"
     CDS             1031..1996
                     /locus_tag="VFQ23_22040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HET9909344.1"
                     /translation="MNTIAVRNLLISLYKIVEASEKGYAVVTSNVKNRALKVLFQSYS
                     QQRLQFKDEIFEEISRLGGDEKSPADNILGMMHRGRIDIFATLTIGDENVERVLLKEV
                     WLGERVALKSYEKTLKEELPPATRSLIESQLQTVRKNVEQIRLLRGQDGKRLVLRLYD
                     SRQDAEQALQSLKQAGVSEKNIKIQDFDPPALEPYKGRGTTLIETVLSGGVGGAVWGV
                     VAGILGAISVMQIAARTQDALSPNMVMIVALGLTAAGIFIGSMVGLFISWGVASQDSY
                     VSETVKKGEVLMETLIDESLASRAWRIMNQVAVAARARHAGESAA"
     gene            complement(1998..2258)
                     /locus_tag="VFQ23_22045"
     CDS             complement(1998..2258)
                     /locus_tag="VFQ23_22045"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HET9909345.1"
                     /translation="MSEEKVHTEEFRVNGEELIGKIKNIIKEGNIRRIIIKDKEGKVI
                     FEIPLTFGILGALLAPQLAAIGAIAALLGEATVVVEKGDKEN"
BASE COUNT          687 a          559 c          586 g          635 t
ORIGIN      
        1 tatcgtaatc atgatgtaat ccgctttcat catcattaat gaagaccgat gccgaaatgt
       61 gcattgcatt gacaagaaca aatccttcct tgacaccgct ttcgcgcaag gcgacctcca
      121 catcaggtgt gatattcaca aatccacgac gggagggaat gttgaaccat agttctttac
      181 gataggattt cattttctat caaaccttcg gaagtaccct gccgaccttt tgcatatatg
      241 aacgatattg gtcaccgaac tgctttatca tcagctgttc ttcggctcta acgcggaata
      301 gatagaaagg gatgaatagc aataggttta aaaaacctgc aatccagttt tgaagaagca
      361 agggctgtgc gatagctaaa agccactgcg acgcatacat ggggtgacga atgacgctgt
      421 agatgccgcg cgtgatcagt tcgtgctgtt ctcgaatctc cagggtaggt gaccagttga
      481 gtcccagatc tgcatgagcc cgccaaaaaa catacagtgc tccggtaagc agaacgacac
      541 ctagccaccc tgcccaggcc ggcaactcgt aattggcgaa atcaagccag ttcgtaaagg
      601 catagataag cgggataaaa aagccgccca gtgataataa acccaggatg atcttttcct
      661 gatcggtgac ccggcgttcg ctcatctttt cttgtttacg ttttttgtta agcggcgcgc
      721 gaataaccat ttcaacgact aaggctagaa ggaaaatcgc tgtaaatata tccatgtgtg
      781 tacccgggat ttttgcaact tactatcagt gattcatgcg gcagggatag tatagcagag
      841 aatgtgaatc ttctcatcac tcactctttg gttgaaaaaa ggtgacaggt cagaactgaa
      901 atcgttagac aaaatagcca gcctgataat ttcaatggat tactttatct ttttttaagg
      961 tctgtagaag taagacttga tcaaacgtta gaaagcaaca cctaactaat ctggctgaaa
     1021 gaggtgcaaa atgaatacaa tagctgtaag aaacttacta atttcccttt acaagattgt
     1081 ggaagcaagt gagaagggat atgccgtagt aacttccaac gtcaaaaacc gggcgctgaa
     1141 ggtcctcttt caatcctact ctcagcaacg ccttcagttt aaggatgaga tctttgaaga
     1201 gatcagtagg ttgggcgggg atgagaaaag tcctgcggat aacattttag gcatgatgca
     1261 ccgcggtcga attgacattt tcgccacgtt gacgattggc gacgaaaatg tagaaagagt
     1321 gctcttaaaa gaagtatggc ttggcgaacg ggtagctctc aaatcttatg aaaaaacctt
     1381 gaaagaggaa ttgccacccg ccacacgcag cctgattgaa agtcaacttc agacagtgcg
     1441 aaaaaatgtg gaacaaattc gcttgttacg cggacaggac ggaaagcgat tggtgcttcg
     1501 tctgtatgat tcgcgacagg atgcagagca ggcgttgcag tcgctgaaac aagcgggcgt
     1561 ctcagaaaag aatattaaaa tccaagactt cgatccacct gctctggaac cctataaggg
     1621 tcgaggaacc acgttaatcg agaccgtact ttccggaggt gtgggaggtg ccgtatgggg
     1681 tgtggttgcc ggtattctgg gagccattag tgtgatgcaa atagcggcgc gcacacagga
     1741 cgccctctca ccaaacatgg ttatgattgt agccctgggt ttgaccgcag ccggtatctt
     1801 catcggcagt atggtcggcc tatttatcag ctggggcgtt gccagccagg acagttatgt
     1861 ctcagagacc gtcaaaaaag gcgaagttct catggaaacg ctgatcgatg agtcactcgc
     1921 atctagggcg tggagaatca tgaaccaggt cgcggtggct gcacgagcaa gacatgcagg
     1981 tgaatcggcc gcctagatca attttctttg tcaccctttt caacgacgac cgttgcttca
     2041 cccagaagcg ccgcgatggc gccaatcgca gcgagttgcg gagccagcag cgcgccaagg
     2101 ataccaaacg tcagcgggat ttcgaaaatg actttgcctt ccttatcctt gatgataatt
     2161 cgccggatat tcccttcttt gatgatgttc ttgatcttgc caatcaattc ttccccgttt
     2221 acgcggaact cttcggtatg aactttttct tcagacataa ctcctccaaa ttttgaaaat
     2281 ttctaccact tatgtctacg agctgtctat ttcaaggttg caaatttata tgagttgata
     2341 ggcacgcaac attccctcaa atgcctgcct gccatcggca agtgcttttg cacgccaatt
     2401 gccttctgat ggaaaatata attcttcaaa ctcccggcga acaagttttg ctgtatgttc
     2461 acttatt
//
LOCUS       DASTJP010000758         5464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_965594, whole genome shotgun sequence.
ACCESSION   DASTJP010000758 DASTJP010000000
VERSION     DASTJP010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965594"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /locus_tag="VFQ23_22050"
     CDS             complement(<1..1988)
                     /locus_tag="VFQ23_22050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909346.1"
                     /translation="MKPRWLKVFHDLIDNKGRTLLVVFSIAVGVFSIGVIAGAYQIIS
                     SDLGLSYSSKHPANIEMRMSNFDEDVRDSLRNQPEVDQVEARRVFNVRVRVPGSESWT
                     TLDMTAFDDFEKNNINLLTQIDGRIIPEKREILLEQDALQRLDVGLGGILEFQLPDGS
                     TKTLPVVGIVQDTASGAGDFLASPAAYVTMDTLQFLGHPKLYNRAYVTVSGDGDDIFY
                     VREVGAELKNKIEKGDTVVIRSRFSKTHEHPLADTLNAVLGILMALGILIVFLSSSLI
                     ANTLSALLNQHLRHIGIIKLVGGQRRQVLRMYLTLIMAFGVLALLIAVPLGGQGAYGL
                     ALFLASELNFDILGYRIVPAALLVQIAVGLLVPLVAGFMPVLNGSRITVLRALSGGLA
                     DEQNQAEVSHAASSAEKRLSWFDWMQIKVTRILAARGIHFPRPFVISLRNTFRRKGRL
                     ALTLFTLTMGGAIFISVFNVRVTLHQYIQQIGKYFVADVTIDFDEPYRRREIEQFVLQ
                     VDGVQRVEGWQFVSGDLLDANDEVLENVNVFGPPADSELVDPLLLAGRWIRSDDVRKF
                     AVSEGALEYFPDLKPGDMLTLRIEGRDEAWEVVGIFQFADREGVLAYAPYEYVAQMNN
                     LVNRSFSYRLVTEQHDRAYQNAKADELDKYFREQGFKIR"
     gene            complement(2055..2807)
                     /locus_tag="VFQ23_22055"
     CDS             complement(2055..2807)
                     /locus_tag="VFQ23_22055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909347.1"
                     /translation="MNMSSSVLGAERRTSEVEGERDPVMIDMQNIVKTFKNAAGEFIV
                     LKGIDLKIDRGEFVSIVGKSGSGKSTLLNMITGIDHPTSGRVVIGGTDIYTGVSESQR
                     SRWRGRNLGIVFQFFQLLPMLTLLENVMLSMDYANLYDFDERPERAMDMLRLVRLEQF
                     ASKLPVLVSTGQQQLAAIARALACDPPLLVADEPTGNLDTKSASAIIDLFEELSRQGK
                     TIVMVTHDPSLTSRTTRNITIADGVLIADSRR"
     gene            complement(2804..3556)
                     /locus_tag="VFQ23_22060"
     CDS             complement(2804..3556)
                     /locus_tag="VFQ23_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909348.1"
                     /translation="MQPNKPLQPIIEVRDVVKVYSTAAGEYEALKKVNLRVGAGEFVG
                     IIGKSGAGKSTLLNMITGVDHLTSGEVIVNSNGDSVSIHKRSEDEIALWRGQTLGVVL
                     QSFQLLPMLTLAENITLPMDLCGVYSPKQSRQRALDLLRLVEIEEHANKLPAYISGGQ
                     QQRVAIARALANDPPILVADEPTGSLDSVTADHILEVFEHLVGDQGKTIVMVTHDQSL
                     ASRFTRTLEIVDGELNDSSMERRDVVLGEMQQ"
     gene            3769..3939
                     /locus_tag="VFQ23_22065"
     CDS             3769..3939
                     /locus_tag="VFQ23_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909349.1"
                     /translation="MARTNVLWNLLVQDALREYGPPIQTSKERPDYTPRMYIVNASSA
                     GDPLILLHHQTL"
     gene            4139..4765
                     /gene="upp"
                     /locus_tag="VFQ23_22070"
     CDS             4139..4765
                     /gene="upp"
                     /locus_tag="VFQ23_22070"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET9909350.1"
                     /translation="MSNVYASKHPLVAHKLSRLRDKNTEPKKFREFVREIAAMLAYEA
                     TADLAIVPRELETPLERMEGAELKEKIGLVPILRAGLGMVEGVWGMMPSAEVWHIGLY
                     RDEHTLRPVEYYNKLPLEPTVSVCLILDPMLATGGSATATAEVLKRWGVKKIKFVGLI
                     GAPEGIKKMQEAHPDIDIYLAAIDDHLNERAYIVPGLGDAGDRQFGTG"
     gene            4787..>5464
                     /locus_tag="VFQ23_22075"
     CDS             4787..>5464
                     /locus_tag="VFQ23_22075"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9909351.1"
                     /translation="MRAMLLREVAPLAQNSTPLSFANIPEPNAAEGEVLIRVTRCGVC
                     HTELDEIEGRTPPPHLPIVLGHQVVGVVESRSSEFNRPVTNEFVTTGQRVGVAWIAAA
                     CGHCHFCLSGQENLCAEFKATGRDINGGYAEYMKIREDFVHRIPDAISDAAAAPLLCA
                     GAVGYRAWKLCNLSEGQALGLMGFGGSNHIVLKTAKHKYPNSSIFVFSRNLNEREFAL
                     SLGAVWAG"
BASE COUNT         1394 a         1448 c         1310 g         1312 t
ORIGIN      
        1 cggatcttga atccctgctc gcggaaatat ttatccaact catcagcttt ggcattctga
       61 taagcacgat catgttgttc cgtcacgagt ctgtaggaga acgaacgatt gaccagatta
      121 ttcatctggg caacatattc atagggtgca tacgccaaca caccttcgcg atcagcaaat
      181 tggaaaatgc cgaccacttc ccaggcttca tctcggccct cgattcgtaa tgtcagcatg
      241 tcgccaggtt ttaggtctgg aaaatattcc aacgcgcctt cactcaccgc aaatttgcgc
      301 acatcatccg agcgaatcca tcgcccggca aggagaagcg gatcaaccag ttcgctgtct
      361 gcaggaggac caaagacatt cacattttcg agtacctcgt cgttcgcatc cagcaggtca
      421 ccgcttacaa actgccagcc ctccacccgt tggaccccat ccacctgcag gacaaattgc
      481 tcgatctctc gtcgccggta gggttcatca aaatcaattg tgacatctgc aacaaaatac
      541 tttccaatct gttgaatata ttgatgcaag gtcacacgta cattgaaaac cgaaataaag
      601 atcgcaccgc ccattgtcaa cgtgaatagc gtcagcgcaa ggcgcccctt ccgtcgaaat
      661 gtgtttcgga gcgagataac aaaagggcga ggaaaatgaa tgccgcgcgc ggcaaggatg
      721 cgcgtcactt tgatctgcat ccagtcgaac catgacagcc gtttctcagc tgaggatgct
      781 gcatgactca cttcggcttg attctgttca tccgccaggc ccccactcaa ggcccgtaaa
      841 acagtaatgc gcgagccatt gaggaccggc ataaagcctg cgaccagtgg caccagtaac
      901 cctactgcaa tttgtacgag caatgccgcg ggtacgatcc gatagcccag tatgtcgaaa
      961 ttcaactcac tggcgagaaa caacgcgagc ccataggctc cctgtccgcc gagcggaacc
     1021 gcgatcaaga gtgccagtac accgaacgcc atgatcaggg tcaaatacat tcgtaggacc
     1081 tggcgtcttt gtccacccac cagtttgata ataccgatat gcctcaaatg ctgattcagt
     1141 aatgcgctca gggtattggc gatcaacgaa ctggatagaa atacaataag aatccctaac
     1201 gccatcagaa ttccaagcac cgcgttgagc gtatcggcga gcggatgctc atgtgtcttc
     1261 gagaaacgcg atcgaatgac cacggtatcg cccttctcga tcttgttctt tagctccgca
     1321 cccacttccc gaacataaaa aatgtcgtca ccatcgcctg agacggtcac ataggcacgg
     1381 ttataaagtt ttggatgtcc tagaaactgt aaagtatcca tggtgacgta agctgccgga
     1441 gaggcaagaa aatccccggc tccggaagcg gtatcctgca cgatgccgac cacgggcagg
     1501 gtctttgttg agccatcggg cagttgaaac tcaaggatac ctcccagccc aacgtccaat
     1561 cgttgaaggg catcctgttc caataaaatt tcacgcttct ctggaatgat tcttccatcg
     1621 atctgagtca gcaaattgat gttgttcttc tcgaagtcat cgaaggcggt catatccagg
     1681 gtcgtccacg attcagaacc cggtacgcgc acacggacgt taaatacgcg acgcgcttcc
     1741 acctgatcca cttcaggttg atttcgaagg gagtcccgta catcctcatc aaagttcgac
     1801 atgcgcattt cgatatttgc cggatgcttc gaagaatagg agaggcccag gtccgaggaa
     1861 atgatctgat acgctcccgc gatcacacca atggaaaaca cacccacggc aatggagaaa
     1921 accaccagca gcgtgcggcc cttgttatct ataagatcat gaaatacttt tagccaacga
     1981 ggtttcataa atatcttcaa aacgtggttt cgatacgtcg gacttacacc gccgactcaa
     2041 ccagcgcgtt gcttctatct cctgctgtcc gcaatcagca caccatcagc aatcgtgatg
     2101 ttacgcgtgg ttcgcgaggt caacgacgga tcgtgcgtaa ccatcacaat ggtcttgccc
     2161 tgacgtgaca attcttcaaa cagatcaata atggcactgg cagatttcgt gtccagattt
     2221 ccagttggtt catccgcaac taacaatgga ggatcacagg ccaacgcgcg tgcaatggct
     2281 gccagttgtt gttgtcccgt ggaaaccaaa acgggcaatt tacttgcaaa ttgctccaac
     2341 cgaaccagcc tgagcatgtc cattgcgcgt tcaggacgtt catcaaaatc gtacagattg
     2401 gcgtaatcca tggagagcat gacgttctca agcaaagtga gcattggcag caattgaaag
     2461 aattgaaaca caatccccag attacgcccg cgccatcggg agcgctgact ttcgctcaca
     2521 cccgtataaa tatccgtgcc gccgatgacg accctgccgc tggtgggatg gtcaatcccg
     2581 gtgatcatgt tcaacaaggt agatttgccg ctcccagact tgccgacaat cgaaacaaat
     2641 tcgccgcgat cgatttttaa gtcaatgccc ttgagaacaa taaactcgcc tgctgcattc
     2701 ttgaatgtct ttacgatatt ctgcatatcg atcatcacag gatctctttc gccttcgact
     2761 tcactcgtgc ggcgctcggc acccaggaca gaagaactca tattcattgc tgcatctccc
     2821 ctaacacgac gtcacgccgc tccatggatg aatcgtttag ttccccatcc acaatttcca
     2881 acgtacgcgt gaagcgcgag gcaagggatt gatcatgagt caccatcacg atcgtcttac
     2941 cctggtcgcc aaccagatgc tcaaagacct ccaaaatatg atctgccgtg accgaatcaa
     3001 gacttcccgt tggctcgtct gcaaccaaaa tgggagggtc attcgccagc gcacgcgcaa
     3061 tggcaacacg ttgctgctgc ccgccagaaa tataggcagg cagtttattc gcatgttctt
     3121 ctatttcaac caggcgcagc aaatccagtg cgcgttgacg cgattgcttc gggctgtata
     3181 ctccacataa atccatcggt agagtgatat tttcagccag cgtgagcatc ggcaatagtt
     3241 gaaaggattg cagcaccacg cccagtgttt gaccgcgcca tagggcaatt tcgtcttcgc
     3301 ttcgcttgtg gattgaaaca gaatcgccat tggaattgac aatcacttcg ccagatgtca
     3361 gatgatcgac tccggtgatc atattgagca atgttgattt gcctgcgccg gatttgccaa
     3421 taattcccac aaattcaccg gcaccgactc gcaggttaac tttcttaagc gcctcatatt
     3481 caccggctgc agtggaatac actttcacga cgtctcgcac ttcgatgatg ggttgaaggg
     3541 gtttgttcgg ttgcatcgtt ttttttattc cccaatggga atgactctag gtcaactgat
     3601 atacaaaaac ggtcgccagg gcaaccgttt tgcagacttg ccgtatttat gattttaccc
     3661 ctgtcactta tttgtgacaa atttcctaat cttacactta tgcaaattat gatggagccc
     3721 ggatttaaag atccgggctc catcagctac atcatatgaa ttacaagcat ggcacgtact
     3781 aacgtgctat ggaaccttct cgttcaggat gcacttcgcg aatatggtcc tcccattcag
     3841 acttcgaagg aaagacctga ctacacacct cgcatgtata ttgtgaatgc atcgtccgct
     3901 ggtgatcctc taattttgct gcatcatcaa actctgtagc acagatttga cacttgtgaa
     3961 cttgagccat ggaatttttc tcctttcaga tgatttagta ttaattatgt ccgcaccggt
     4021 tcccgggtca atgaggattt tcccagccgg agcagggaaa tccccacctt cttcccaatc
     4081 catagctata attttcgagc aggcgggcta taataaagta atcaatcctg gaggagccat
     4141 gtccaacgtc tacgcgtcca aacatccgct ggttgcccac aaactatccc gtttacgaga
     4201 taagaacacc gagccaaaga aattccgtga gttcgtgcgc gaaatcgcgg cgatgctcgc
     4261 ttatgaagct actgccgatt tagcaattgt gccaagggaa ttggaaacgc ctctcgagag
     4321 aatggagggc gctgaattaa aagaaaaaat cgggctcgtc cccatcctgc gggcgggcct
     4381 gggtatggtg gaaggtgtat ggggaatgat gccgagcgcc gaagtgtggc atatcggttt
     4441 gtatcgcgat gaacatacgt tgcgacctgt ggaatattac aacaagctac cgctcgaacc
     4501 caccgtttcg gtttgcctga tcctcgaccc gatgcttgcc accggcgggt cggctaccgc
     4561 aaccgcggaa gtgcttaaac gctggggggt aaagaagatc aagttcgtcg ggctcattgg
     4621 cgcgccggaa ggaattaaga agatgcagga agcgcaccct gatattgaca tctatctcgc
     4681 cgccatcgat gatcatctca acgaacgcgc gtacattgtg cccggtctgg gcgatgccgg
     4741 agaccgtcag tttgggacgg gataacttaa cgtcgaaata acctctatgc gagccatgct
     4801 gttgcgcgag gtcgcgccgc ttgcgcaaaa ctcaacgccg ctttcatttg caaatatacc
     4861 tgagccaaac gctgctgaag gcgaagtgct gatccgtgtg acgcgatgcg gagtctgtca
     4921 tacggagttg gacgaaatcg aagggcggac tccccctccg catctgccca tagtactggg
     4981 acatcaggtt gttggagtcg ttgaatctcg tagtagcgaa ttcaatcgcc ctgtaacgaa
     5041 tgaattcgtt accacaggtc agcgcgtggg tgtggcatgg atcgccgccg cgtgtgggca
     5101 ttgccacttt tgcttatctg gtcaggaaaa tttatgcgcg gaattcaaag ctacgggccg
     5161 tgatatcaac ggcggatatg ccgaatacat gaagatccgt gaggattttg ttcaccggat
     5221 ccccgatgca atttcagatg cggcggccgc gccattatta tgtgcgggag cggtgggtta
     5281 ccgcgcatgg aagttatgta atcttagcga gggtcaggca ttaggtttaa tgggattcgg
     5341 cgggtccaat cacatcgtat tgaaaactgc aaaacataaa tatccaaact catcgatttt
     5401 tgtctttagc cgaaacctta atgagcgaga gttcgcgctt tcgttgggag ccgtctgggc
     5461 aggc
//
LOCUS       DASTJP010000759         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1166393, whole genome shotgun sequence.
ACCESSION   DASTJP010000759 DASTJP010000000
VERSION     DASTJP010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166393"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFQ23_22080"
     CDS             <1..185
                     /locus_tag="VFQ23_22080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909352.1"
                     /translation="VDRVSLKLHSKLRLLFFGSHTAAQHSPPGKNTLGDDVPLRYAPG
                     MITPAVSPHSLPVIQL"
     gene            199..1146
                     /locus_tag="VFQ23_22085"
     CDS             199..1146
                     /locus_tag="VFQ23_22085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909353.1"
                     /translation="MKTLTTRLYETERDLQQMYDLLQEARSLTNDWRYSHVGELAFNY
                     FMVAIHLNPQEHIRLWHDNGKLVAYAILGEDPAFDWQVLPEYEWSGIELEALLWAEAR
                     TDELRKQDEGRWGADLVSGSRQDDIHRIAFLEQHGFQYCGDFAEVNMMRLLEEPIPES
                     RLPDGCQVREVSETGEIAARASIQREVWHPWTVGNVSDDDYARFMRLPGYYRDLDIVS
                     VAPSGAIAAYVNGWIDPLNKIGDFGPVGALEAYRRQGLTRAALLEGLRRMKAYGMNRV
                     CISTGVSNVPAKQLYESIGFRMVNRYLDYVKLREKQKAA"
     gene            1392..1994
                     /locus_tag="VFQ23_22090"
     CDS             1392..1994
                     /locus_tag="VFQ23_22090"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9909354.1"
                     /translation="MIVLYLGLVGLLAFIMGAGVLGLWLRKHPSKPNAERSSRIMHFL
                     FFAGLGTPFLVSLFYPGLAHLDELIGLKPLPWRSVFLIAGIVLAVPGLYFLAVSNKSL
                     RALGSGTNAFRLTKHIVEGDIYKYTRNPMSLGYYLFSLGVGFISGSTLLTLYVLFGLI
                     PAHLFFLRFFEELELELRLGESYKEYKQKTPFLIPSPSEK"
     gene            2318..>2573
                     /locus_tag="VFQ23_22095"
     CDS             2318..>2573
                     /locus_tag="VFQ23_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909355.1"
                     /translation="MNEKIEVDELASWVVIIFITLFGGWLRILLLADKGMWLDETFSV
                     WIASHSVPDMLQWIVKIDQHPPLYYLLLHYWMAHNGDTPYY"
BASE COUNT          619 a          626 c          661 g          667 t
ORIGIN      
        1 atgttgaccg ggtaagcttg aagctgcact caaaactcag gctactattc tttggatccc
       61 atactgctgc ccaacacagc cctccaggga agaacaccct gggggacgat gtcccactcc
      121 gctacgctcc ggggatgata acgccggccg ttagcccgca ctctctgcca gttattcaat
      181 tatgatgtaa aagaaacaat gaaaacgttg acaacccgtc tctatgaaac cgaacgagac
      241 ttacagcaga tgtatgatct actgcaggaa gcgcgttcac tcacaaatga ttggcgctat
      301 tcccatgtcg gtgaattagc cttcaattac ttcatggtcg ccatccatct caatccacag
      361 gaacatatcc gtctctggca tgacaatgga aaactggtag cgtacgcaat tttaggcgaa
      421 gatcctgcct tcgattggca ggtattgcca gagtatgaat ggtccggtat cgaactcgaa
      481 gcgctactgt gggcagaagc acgtacagac gaacttcgaa aacaggatga aggaagatgg
      541 ggcgctgatc ttgtttcagg ttcacgtcag gatgacatcc atcgaattgc tttcctggaa
      601 caacacggtt ttcaatactg cggtgatttt gcagaggtca atatgatgcg cctgttggag
      661 gaaccaattc ccgagtcacg gttgcctgat ggttgtcagg tccgtgaagt ctcggaaaca
      721 ggggagatcg cggcccgcgc ctccatccag cgcgaagtat ggcatccctg gacggttggc
      781 aatgtcagcg acgatgatta cgcacgcttt atgcgattac ccggttatta tcgcgacctt
      841 gatattgttt cggttgcacc cagtggagct attgccgcgt atgtgaatgg atggattgac
      901 ccacttaata agattggtga ttttggaccg gttggagcgc ttgaggctta tcgcagacaa
      961 ggattgacgc gcgccgcact actggaagga ctgcgccgaa tgaaggcata tggaatgaat
     1021 cgcgtgtgta tctctactgg tgtatcaaat gtacctgcaa agcaactcta tgaatcgatt
     1081 ggtttcagga tggtcaacag gtatcttgat tatgtaaaat taagagaaaa gcaaaaagcg
     1141 gcctaacaca gtcccgcctc gtgcgtcgct gttgcgacag tcttcgacgt gcaccggggg
     1201 aaggtgtgcg caatcccact cgggaagaac gccctcggga agaacaccct cggctgcaca
     1261 gcctttcagg gtggggacga tgtcccactc cactacgctc cggggatgat aacgcaagca
     1321 gttgggcaga gctttttagt tgtcgaggta tgatgtagca gcatacccgg tgtgattgaa
     1381 ggaggtgtca aatgatcgta ctctatctgg gtcttgtcgg tctcctggca tttatcatgg
     1441 gcgccggggt cctggggtta tggctacgaa agcatccctc caaaccgaat gctgaaagat
     1501 ccagtcgtat catgcatttt cttttctttg ccgggttagg aacgcccttc ctggtcagcc
     1561 tcttctatcc cgggctcgca cacctcgatg aactcattgg tctgaagcca ttgccgtgga
     1621 gatccgtttt tcttatcgcg ggcattgtat tggcggtacc gggtttgtat ttcctcgcgg
     1681 tatccaataa atcactgcga gctttgggca gcggaaccaa cgccttccgt ctgacaaagc
     1741 atattgttga aggagatatt tacaaataca cccgaaatcc aatgtcgctt ggctattatt
     1801 tattctcgct gggcgtgggt ttcatttcgg gatcaacact tttgacgctt tatgtactct
     1861 tcggtctgat tcctgctcat cttttcttcc ttcgattctt tgaagaactt gagctggaac
     1921 tccgccttgg agagtcttat aaggaataca aacagaaaac accgttcctt attccgagcc
     1981 cttcggaaaa gtagttgtaa aattcgctct gcacaacccc gcgtgcaccg aacagccggg
     2041 tctcttctcg gcgggcggtt gataatcacc gccgcacagg tagaattaca gccgggttcg
     2101 gatttagaac gctccttgat ccccctgtcc aggtggatag gatgaaactc ccccgtggta
     2161 gggggagttc ctgtttagaa tggggtacca tgaattttgc ccgcagtcat ctgaatagcc
     2221 tcaccctgag caagaaactt attcgggcaa atcacggtcg aatatggaaa aagcttgctt
     2281 tcgggtacat ctgtcaatta caggattctt aaagacgatg aacgaaaaaa tcgaagttga
     2341 cgagttggcg tcctgggtgg taatcatctt catcacattg tttggtggct ggctgcgaat
     2401 tctgctgctc gcggataaag ggatgtggtt ggatgaaacc ttcagcgtgt ggattgccag
     2461 ccacagtgtc cccgatatgc tgcaatggat tgtcaagatt gaccaacacc caccccttta
     2521 ttatctcctg ctccattact ggatggcaca caatggggat acgccctatt atg
//
LOCUS       DASTJP010000760        22910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_45266, whole genome shotgun sequence.
ACCESSION   DASTJP010000760 DASTJP010000000
VERSION     DASTJP010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 22910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22910
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45266"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2804
                     /locus_tag="VFQ23_22100"
     CDS             <1..2804
                     /locus_tag="VFQ23_22100"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9909356.1"
                     /translation="YVGPALNRVARLLSAAYGGQVLLTQPVYDLVRDDLPFDIDLRDL
                     GEHRLKDLVRPEHIYQLTAPGLQSDFPPLKTLDSFPHNLPLQVTSFIGREKEMAEVKR
                     LLWDGRLVTLTGPGGTGKTRLSLQIAADFLELYPDGAWLIELAPLADPALLPQTVANV
                     MGIRESSGHPVMSLLTEHLRAKELLLILDNCEHLVEACAHLVDTLLKACPDVLILASS
                     REALGIAGEASFRVPSLSTPDARHLPPFETLTQYEAVQLFIERAEMAMPGFTVTKENA
                     SAIAQICYRLDGIPLAIELAAARIQVLRVEQIASRLDNRFRLLTGGSRTALPRHQTLR
                     ALIDWSYELLSTSERALLGRLSVFAGEWTLEAAEAICIGEGIEADEIMDFLIQLVNKS
                     LVNSDRAHTAEARYHLLETIRQYARDKLLEAGGGERIRDQHLDFFLKMSERAEPDLRG
                     PDQVKLLNMLEDELDNMRAALEWSLERDIEKGLRLASSLMWLWHLRGHASEGIEWLDR
                     MLTSPIGEDDKTPGLQTRAKALLMLGLLRMFLSNPGAALQPLQESQKLYEELGADGKQ
                     GKAYAIWGLGTTASYKGNNDKAKALTEESLALFRELGDKFGITECLNTLGDIALTQGD
                     YEQAKLLWQESLVLKRERGDKDGIAFSLAQLGNIAFLEGEFERATPLYEEAATLFRQV
                     GNKFFASAAFYSLGETAWAQGNYAHAAKKYEEALKLGKGVDEERAVAFVLYDLGAMAR
                     SQGNYDQAVKMLEEAVTLFKEIGNKWGISTAYYGIGEVAWARGQYDLAARKFEESLVL
                     SRELDAKFNIALALYGLGKVAQSRGDYESAHNFHREALTIRRGTWDRPGIARSLESFA
                     ALAVAEGHMERAARLLGAMETAHELMRFTVSPAERAEREHGMNSSRTALGDDAFANAY
                     DEGKKMTLDEAVGFALGES"
     gene            complement(2918..3994)
                     /locus_tag="VFQ23_22105"
     CDS             complement(2918..3994)
                     /locus_tag="VFQ23_22105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HET9909357.1"
                     /translation="MKHKFVSVIVSLLFLLSITFTNAYSVEAQSDIIVVTTTIQAAVD
                     AASPGDTVRVPPGVYRENVFVTKNNITIEGSVGAILDGTGLPGTSGITVRSLIPSARI
                     NGFRLTGLLIQNYVRNGVILNRVDNYQIDHGVYVNNKAYGIFPIRSTNGLIEFNQVSG
                     SDDTGIYIGQSSGTIIRKNHVTDCTVGISAEVSSYITIQDNKVMDNTIGILAAVLPGL
                     SMTETANVQINDNVIMRNNRPNPVTDPADILSQLPSGVGLLIFGADQSIVTGNKVLDN
                     NSVGIAVIQVPPALAALDPRIDPFPDDNQISRNIVLSNGSNPDPKIAPFPASDLIWDV
                     SGTGNCWSDNNYKTSFPLLLPVCQ"
     gene            complement(4076..5161)
                     /locus_tag="VFQ23_22110"
     CDS             complement(4076..5161)
                     /locus_tag="VFQ23_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909358.1"
                     /translation="MNVITRVSIILVVFSILMSGCGAAPTPTPMAVPPTASVSTEAPL
                     ATASPIPTVIPPTQTESTPGILAIVPVAMGPTTLAATSDSIWVESHREDIVTRIDPLE
                     NKEVTRLEDAPVHCSVASGGGFVWAAQAHSDGSPSLDEGRVTKIDPASGAVVGSIELP
                     TACGVTADDNDLWVTSPMGSKVVRYDVATLQERAVIPLAADIFEIKIGPEAVWALGAS
                     DGGTVWRIDPATNQVMTSISISDPFFIGLEVAFGSVWVGSRAKGTLYRIDPATNAVTE
                     TIKVNNSIGGIGVGPDAIWASGFGDGTIYRIDPATNSISGSLSTRYWNLGPPLVAFDS
                     IWVGALDQNVVLRIDPAAFDSSAATTP"
     gene            complement(5358..8159)
                     /locus_tag="VFQ23_22115"
     CDS             complement(5358..8159)
                     /locus_tag="VFQ23_22115"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET9909359.1"
                     /translation="MVRSSYRVYLLGSFQIEYVDSTDASVELIHLPRRKAEALLSHLI
                     LHPQTHSREKLAALLWGDFSDAQARASLRNALAILRKALGENLFLTEHDLIQINPDYP
                     LWVDIREFERQTDPEAAIALYRGDLLSDFYDDWILAFRDHYRELYLSALLNLTQAYRA
                     QSEYERAIEIAKKILEKDPANERAHQQLMFCYMASGDRQAALEQYGKAERVLQEEFAI
                     EPAPETNALNHWIKQTPSDVPSFAARITNLPIPLTSFIGRKQESREVKKLLAHSRLLT
                     LTGAGGSGKTRLAIQVATDLIDQFKDGVWWVDLAPLTDEVLVPQIVAKALGVREVPNQ
                     PLLETLAGFLDEKQLLLVIDNCEHLITASAQLANHLLTQCSNLCLLATSRELLNITGE
                     TVWPVPKLSVPETETLSLTELLMSYESIRLFVERAQSVSADFELTTENALSVVQICRR
                     LDGIPLAIELAAARVKVLSPAQISARLDDRFNLLTRGSRTALPRQQTLRALIDWSYDL
                     LSENERVLFRRLAVFAGGFTLDAAGAICGADNFKESELLDCLSGLVEKSLIIVEKQRH
                     EKRYDVLETIRQYAADRLLDSREEERIRANHRDYFLAMAEQAEPRGLWGAVEITWLNR
                     FELEAGNLRAALEWSMQHGEAEEKLHFAWLIVWYWYVRAQFSEGRRWLEQALTTSNGA
                     SASTRALALMGLGNLVEQQGEYARAAELLEEALRLFHELKNSWRAAWTLQHIGLVALF
                     LSDYHRATSAFTESLTLFRERQDKGGIATLLMYLGITACYKGENEGALALLEESLPML
                     RELGDEVGIARALHGLGLLAQQKGDFDNAASMYAEGLMLAQQRGARAEVADYLRGFAD
                     LACAQNKFERAARLFGAEATLREAIGYPVPSIEAPVHEQNLSTVRALLGEEKFSQAWA
                     EGHVMEWTQASEYALSERE"
     gene            complement(8300..8497)
                     /locus_tag="VFQ23_22120"
     CDS             complement(8300..8497)
                     /locus_tag="VFQ23_22120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909360.1"
                     /translation="MLKSPRFMVGLGLILMLLGIVLPFLILIKVLESTFFLNFFAYGA
                     SVGGLFMGVIGVAYWVRTKKE"
     gene            complement(8498..9559)
                     /locus_tag="VFQ23_22125"
     CDS             complement(8498..9559)
                     /locus_tag="VFQ23_22125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909361.1"
                     /translation="MSASETKDAETLLSIQDLHVWYELRRFGFGHAGYVKAVDGVSFN
                     MEEGETVAVVGESGCGKSSLMKTILGLNIPTSGDVVFDGENLSDLKGNEVQRYRFKVG
                     YVQQDPYGALPPFMNVQRILEEPLLISGMKDPEERLARIHKVMTEVKMHPVDDFLQKF
                     PHMLSGGQQQRIVIARAMLREPKLIVADEPVSMLDASVRVEILKLMRALQQSHGLSVI
                     YITHDLSTVKYFSKRIFVMYAGNLIEKAETRNLLENPLHPYTHALLAATSDPDARNAD
                     SFKEVPPGEPPSLVNPPTGCRFHPRCPHFMAGLCDVKFPPDFEPEPGHFVACWLYDKP
                     EALDAFRNPNGNTQTKSDD"
     gene            complement(9735..10316)
                     /locus_tag="VFQ23_22130"
     CDS             complement(9735..10316)
                     /locus_tag="VFQ23_22130"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9909362.1"
                     /translation="MSSLALYDILYHFKDIHKVVLNLAESLTDEQLTWKPEGYSTSIG
                     FHLWHLARESDYLKAAILEFFPGLELEVENSGEIWSRNGLARKWEFPIEAHETVGTGL
                     SDEVAASLPIPPKDELLDYLRNSYNAIEEFVEQLDTKYADFEHLDEELRKKVERIRLN
                     LLVFLTHDCRHLGMMECLKGLQTGFGSATEKRN"
     gene            complement(10325..10525)
                     /locus_tag="VFQ23_22135"
     CDS             complement(10325..10525)
                     /locus_tag="VFQ23_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909363.1"
                     /translation="MEKFISFFWFIATVTSWVSFGFASWEWADSYNGQVYDSGMLVLM
                     ILWFVMSLVLFLVGLRLRKPSA"
     gene            complement(10537..11499)
                     /locus_tag="VFQ23_22140"
     CDS             complement(10537..11499)
                     /locus_tag="VFQ23_22140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909364.1"
                     /translation="MNSNGHKLLQIEKLVLHFKTRGGVVQAVDGVNFELDSNRAVVVL
                     GESGCGKSSLAKAILRLLPRNVDKYSGHVHLQGTDVMKFDEEEFRKNVRWVGISLVPQ
                     AAMNSLNPVLKVGDQVAEPAITHLGLNRADAIGLVHKMFQHVGVPRDFVERYPFELSG
                     GMRQRVALAMALVTSPALIVLDEPTSALDLLTQANIMNVLKRIKQELGTSFILITHDI
                     ATSSELADEVAVMYAGQIVEVGHARDFFPAPLHPYAQMLMASVPRLRSDSDPMFITGQ
                     PPSLLNPPKGCRFAARCPFRFEKCDEEPPMFGKNGRKVKCWLHA"
     gene            complement(11496..12833)
                     /locus_tag="VFQ23_22145"
     CDS             complement(11496..12833)
                     /locus_tag="VFQ23_22145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909365.1"
                     /translation="MNTWRGSLQRLFQYPSAVVGTFVVFLLVFTAIYAMVKIPYREAI
                     RLWRGGEEVWYQNPKFAPPAWVNLFSSKKYVESFSVRTSDGSLAKTVEGGDQDVTTYT
                     MNYSFDFNYDYYPQEMIVYVTSTFAEKQPFISLEWLKPDGETVRIANLAVGQKHTYRF
                     SQDDKLKTKLRTQEIIPVLFSLPEQNVPEKGTYQLLITGTTFEPNSDINVEFVLHGQV
                     FGLAGTDHARRDLVVPLLWGAPVALAFGLIASLGTAILTMVISALGAWYGTWVDELIQ
                     RITEINLVLPFFSILILIGTFYSRSIWVILGATILLSIFTGAIKGYRSIFVQLKESPY
                     IEAARAYGASNPRIVFLYLIPRMIPLLIPGLVLSVPTFVFLEAGLAVLGLGDPVLPTW
                     GKMIEDANRNAALYRGYYYWILEPTVLLMVTGLGFAMLGFALDRVFNPKLRDA"
     gene            complement(12836..13960)
                     /locus_tag="VFQ23_22150"
     CDS             complement(12836..13960)
                     /locus_tag="VFQ23_22150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909366.1"
                     /translation="MAVVTHTPTENVAPKKARHQSTFVRVSRYVVVRLLTLFVTVVIG
                     IYLTIVIANMGGFVDTIMKGEIRERVTNSVIASPAAQQMDPDVRTKLIQDRIAAEEER
                     LGLNVPTAIRNFRFLSNALTLQLGRAFNMTSDSGSKTVRLIILERLPATLLLMGISQL
                     FLFFSSIFLALNLSRRYGNIWDKIVVALSPTSAVPPWFYGIFLILIFASLLRVLPFGG
                     MVDSPPPSNTFDYSLSLAKHLILPSLALILSAFFLSIYTYRTFFLIYSSEDYVEMAKA
                     KGLPARDVEQKYILRPTLPNIITNFALLIISLWNGAIITETIFLWPGLGRTLFQAVGL
                     YDIPIIVGSTIINAYLLAFTVFLLDFIYALVDPRVKIGGN"
     gene            complement(14075..16696)
                     /locus_tag="VFQ23_22155"
     CDS             complement(14075..16696)
                     /locus_tag="VFQ23_22155"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9909367.1"
                     /translation="MNSKGPYYILSLLVALSMILAACAPAATATEPPAMQETEPPVVA
                     TDAPATDAPATEAPATEAPTAEPTTRRGGWLDEIDISVVDGASAISQIQAGTIDFFSF
                     NLASDVYPAIQDAGLPSTQSLGGYYGISLNPAVFTDTSRLNPFSNRKIREALNWLIDR
                     NYINQEIYAGGSMPRLLPITTQLVEYTNLIETARALESKYAYNPDRAKEVIDAEMPGM
                     GAELGSDGTWQFNGAPVTLTFLIRSDGDGTRQPMGDYVSNQLETLGFTVDRQYKTSSE
                     AFPIWQGTTAADGQWHLYTAGYIPSGLASLRDERGNIQQSYLNTSVQASEPFISNVSD
                     PELQELGDDLANGNFTSKAERDDMMRRALELSMEDSLFVWVIDQQQYAPHADNVQVTY
                     DLATGPESTNVGPYNLRFTDQEGGTMRIGTNDLFTQPWNTVAGSNWVWDAHVMRMTTM
                     GTSNFIGGAGLMADPYTGLAYPQRIDSAELTYKEGLPINQSLDWLTVNAVAQVDVPED
                     AWADWNAAEQRFVTAAERFPDGATANIMSKVTYPADLWDTVKWHDGSPISPADFVMSI
                     IQGFDPAKPESAIYDESLALSIDAGLASFKGIRITSTDPLTIEYYTDSYNSDAELNIL
                     PLWPDSPFGLSGENSWPVLAISNLAEANGELAYTQTKADDAQIENTSWVGGPSLEILK
                     KYLDQAESESLIPFEPTLGEYITAEEAAQRYANLQQWYADHDHFWVGTGPYYLDQVFT
                     TEKTAVLKNNAEFVDLADRWSEFSSPKLATTVLDGPGQVKVGEEAVFDAIVNLNDQPY
                     PQADIARVKYILYDATGAVVSVGDATAVGDGQYQVTLPADVTSNLPTGSARLEVAVVP
                     LPVAIPSFTSFDFVAVP"
     gene            17183..21106
                     /locus_tag="VFQ23_22160"
     CDS             17183..21106
                     /locus_tag="VFQ23_22160"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET9909368.1"
                     /translation="MHKYWKQAGSRILGLGLALALASCGGVESPKNASESIKPGQDVA
                     VSTPINLPLAPSHAPDESLPQICNCVLRFDHISIEQGLSQSSVRVIFQDSRGFLWFGT
                     EDGLNRYDGYEFKAYKPEPSNPNSISSGWINAIVEDQDGYLWIGTSLGGINRFDPLTG
                     GFTQYQHIDRQTNTVRDNHINALLVDQDNRLWIGTLNGLDRLDPETGNFHHYPYNELQ
                     PKTGEGNVNNPDAQTNPDSIRYYPGSTLSNKLSSKNVTAIYEDSSGRLWIGTSDAGLN
                     QFDEESGVFTIYRNIEDLPLSSASKLMISSNRITAITESPQGDLWIGTGNGLNLLDPE
                     TGEFQIFVNDESDTGSLVSNSINTLSTDGTGNLWIGTANGLDRLHEDFEHFIHYNNDP
                     SFQNSLSNNYIISIYEDRGGVLWFGTYGGGVNKYDRGRANFAYYRSDPKDPNSLSGNF
                     IFPIHVDKEGFAWIGTSGDGLNRFNWQTGENTRFVNDMDNLNSISSNSLLSIYEDSEG
                     TMWFGTTKSLDRFDPKQKTFIHYTHDESNPDSISANRVYAIHEDGNGDLWLGTSIGLD
                     RFNKKTGVFTHYNPDPEDPASLSGNKVTAILEDKEGTLWVGTSESGLNRLNLNTGRFT
                     HYRHDSKIENSISSDSILSVYQDSQERLWIGTAGGGLNLYDPETNGFTFYLEKDGLPN
                     GFVYGILEDEKGHLWMSTNFGLSRFDPDSKTFRNFSADDGLQSNEFDSYAYAMGRDGE
                     FYFGGINGLTVFHPSNITDNPYVPQISLISLSQDDQPVTLDSSVEAAQEVTLEWPQNS
                     LEFEFAALSYNQPNKNQYAYMLEGFDTNWHFIGTKRSGRYTNIPGGKYTLLLKATNSD
                     GVWNETPVQLDVTVIPPYWQTVWFRAALSLAVVAIVLGGVKLRTKSIQDRNRALERLV
                     RERTHALEKRGRELEALYQADEKILRNISLNQVFQTLVDVAVDLLHADRSVVFAWDEE
                     QTKVRPRVSHGYSARTLKVMEFARGEGNVGQVLATGKPIIARELEPETMRPDVREAII
                     AEGIKSFVHLPITVDHKVVAVFNVAFTQPNFIGEDTIRLFSALTDRASLSIANMELFE
                     QTKDLAVMEERSRLARDLHDSAKQKAFAALAQLGTARGILNGNGHSVAIHLDQAENLV
                     SDVIQELTFLVQEIYPIAVQEKGLATALREYIFEWENRNDSTAKLSIRNERRLPLEVE
                     QAIYRVTQESLANVARHSDATRVDIALVYNVDSVQLSLSDDGCGFDTDVKSHGMGLRS
                     IRERVSSVHGVAQIQSAPGHGTRILVQIPLKVQEGASNGKQSTYA"
     gene            21081..21770
                     /locus_tag="VFQ23_22165"
     CDS             21081..21770
                     /locus_tag="VFQ23_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909369.1"
                     /translation="MANNQHTHSNRTSILIVDDHEVVRNGIRSYIETVKEFQVVGEAA
                     SGEEAVRLVSEHIPDIVLLDLIMPGMDGVETTRHIKQISPRTQVVVLTSYHEDVHIFP
                     ALKAGAISYILKDMKMEKLVDALYRAAQKEVTLHPLVAARVLQNIRSDNSEEQPLFTE
                     LTERELDVLRLIANGMTNSQIAEKLVISEYTVKGHVSNILSKLHLADRTQVAVYAWQR
                     GLVSREQMGKK"
     gene            complement(21910..22482)
                     /locus_tag="VFQ23_22170"
     CDS             complement(21910..22482)
                     /locus_tag="VFQ23_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9909370.1"
                     /translation="MTNSNTELVTFFKALADANRLKIVGLLAEKPYSVEELAALLNLK
                     ASTVSHHLSRLAEAELVTSRTESYYSVYQLDQSVLQEKARTMFSQNELSSVVADVDAD
                     AYDNKVIKDYSKRDGSLKTLPAQRKKLDAVLRHVVKSFESGKRYSEKRVNEILAGYYE
                     DTATLRRELVGSGLMKREGGGGEYWRVEIN"
     gene            complement(22683..>22910)
                     /locus_tag="VFQ23_22175"
     CDS             complement(22683..>22910)
                     /locus_tag="VFQ23_22175"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HET9909371.1"
                     /translation="NADGTIRQIPQEGKKMVIMLNFLLDAFSFDTNYTEKEVNTILRR
                     FHVDTATLRRNLIDYGFLDRESDGTRYWRIK"
BASE COUNT         5856 a         5779 c         5800 g         5475 t
ORIGIN      
        1 attatgtagg tccggccctc aatcgcgtcg cgcgtttgtt gagcgctgcc tacggtgggc
       61 aagtgctgct aacacagcct gtctatgacc tcgtacgtga tgatttgcct ttcgacatcg
      121 acttgcgtga tttgggagaa catcgtctca aggatctagt ccgtccggag catatctatc
      181 aattgactgc gcccggtctc caatccgatt ttcctccgct aaaaacgctt gatagcttcc
      241 ctcataactt acccctgcag gtgaccagct tcattggtcg tgaaaaagag atggcagaag
      301 tgaaacgtct gctctgggac gggcgtctgg tcacactaac aggtcccggt ggaacgggga
      361 aaacacgtct ctcattgcag attgctgctg actttctgga gctctaccca gacggcgcgt
      421 ggctgatcga gttggcaccg ctggcagacc ctgctctatt accacaaaca gttgcgaatg
      481 tgatgggaat acgtgaatcg tcagggcacc cggtcatgtc cctgttgaca gagcacctgc
      541 gagcgaaaga gttgcttctg attctggata actgtgagca cctggtcgaa gcttgtgctc
      601 atctggtgga tacattgctt aaagcctgcc cagatgtctt gatcctggct agcagccgcg
      661 aagcactggg cattgcagga gaggcgtcgt tccgcgtgcc gtcgctgtca acaccggatg
      721 cgcggcattt gccacccttt gaaaccctga ctcaatatga agcggttcaa ttattcattg
      781 agcgcgcgga aatggcaatg cccggcttca cagtaacaaa ggaaaatgca tccgccatcg
      841 cgcaaatctg ttaccgcctg gatggaattc cgttggccat tgaactggca gctgcacgta
      901 tacaggtcct tcgcgtggag cagatcgcat cgaggctgga taatcgcttt cgattactta
      961 caggcgggag ccgcacagcc ttgccgcgcc atcagacctt gcgcgcatta atcgattgga
     1021 gttacgaact tctatcgact tccgaacgtg ctctcctggg ccggctgtct gtgtttgccg
     1081 gcgagtggac gctggaagct gcggaggcaa tttgtatcgg agagggaatc gaagctgatg
     1141 agattatgga ttttttgatt caactggtga acaaatcatt ggttaattca gatcgcgctc
     1201 atacagcaga ggcacgctat catttacttg aaaccatccg ccaatatgcc cgggataaat
     1261 tactggaggc cggcggcgga gaacgtattc gagaccagca tttggatttt ttcctaaaaa
     1321 tgtctgaacg tgctgaacca gatttacgcg gccctgacca ggttaagttg ctcaatatgc
     1381 tggaggatga actggataac atgcgcgctg ccctggaatg gtctctggag cgcgatattg
     1441 aaaagggact gcggcttgcc tcgtctctga tgtggttatg gcatttgcgc ggtcacgcca
     1501 gcgaaggcat cgaatggctg gatcgtatgc tgacctcccc aattggagag gatgacaaaa
     1561 caccaggtct gcagacgcgc gcaaaagcac ttctgatgct tggtctgttg cgcatgtttc
     1621 tttccaaccc aggcgctgca ctgcaaccgt tgcaggaaag tcagaagtta tatgaagaac
     1681 ttggcgcaga cggaaagcag ggtaaagcct atgcaatatg gggtcttggg accacggcat
     1741 cctataaggg aaataacgac aaggcaaaag cgctcacaga agaaagcctc gcactatttc
     1801 gggagctagg cgacaagttc ggcattacgg aatgtctcaa taccctggga gatatcgcgt
     1861 tgactcaggg agattatgaa caggcaaagc tactgtggca agagtcgctt gtgctgaaaa
     1921 gggagagagg cgataaggac ggaatcgcat tttcattggc gcaattgggc aacatcgcgt
     1981 tcctggaggg tgagttcgaa cgagccacgc cactgtatga ggaggccgcc acgctgtttc
     2041 ggcaggttgg gaataaattt tttgcgtccg ctgcctttta ctcgttggga gaaaccgcct
     2101 gggcacaggg gaactatgca cacgcggcta aaaaatatga agaagccctt aagcttggga
     2161 aaggcgtaga tgaagagagg gcagttgcgt tcgtcctcta tgatctggga gccatggcac
     2221 gctctcaggg taattacgac caggccgtca aaatgctgga ggaagccgtt actctattta
     2281 aagagatagg aaacaaatgg ggaatatcaa ccgcctatta cggcatcggt gaagtagcct
     2341 gggccagagg gcaatatgac ctggcagcca ggaagttcga agagtcgctg gtattgagcc
     2401 gggaattgga tgcaaaattc aacattgcac ttgcactgta tggattgggc aaagtggcgc
     2461 agtcacgcgg cgactatgag tctgctcata attttcatcg tgaggcactg acgatccggc
     2521 gcggaacctg ggaccggcct ggcatcgccc gctcactgga atcatttgca gccctggcag
     2581 tcgccgaggg acacatggag cgagcagccc gattacttgg cgccatggag actgcccacg
     2641 aattgatgcg ttttacggtc tcccctgctg agcgtgcgga acgtgaacat ggtatgaaca
     2701 gttcacgtac tgcgttggga gatgatgctt ttgcaaatgc ttatgatgag ggaaaaaaga
     2761 tgacacttga cgaagcagtc gggttcgcgc tgggagaaag ctgattactt tattgttttc
     2821 ttttcgtgat tctgcagagt ctcagacaat cctttaagaa aaacaaacgt gaccgtaggc
     2881 atactcacgg tcacgtttct acttgtatcg ctacttatta ctggcaaaca ggcaacagca
     2941 agggaaacga tgttttgtaa ttattatcgc tccaacaatt gcctgttccc gacacatccc
     3001 aaatgagatc acttgcaggg aatggagcaa tcttcgggtc aggattactt ccgtttgata
     3061 aaactatatt gcggctaatc tggttatcgt caggaaaagg atcaatgcgc gggtctaacg
     3121 cagccagggc aggtggaact tgaatcacag cgatgccaac actattattg tcgagtacct
     3181 tgttcccggt cacaatactt tgatcggcac cgaaaatcag aaggccaacg ccgctcggaa
     3241 gttgtgagag aatatcagca ggatctgtta ccggattcgg acggttattc cgcatgatca
     3301 cgttgtcgtt aatttgaacg ttcgccgtct ctgtcatgct cagtcctggc agaactgcag
     3361 caagtattcc gatggtgttg tccattacct tattatcttg aatggtgata tacgagctaa
     3421 cctcagcgct gatgcctact gtgcaatctg tgacgtggtt ctttctgatg attgtaccgc
     3481 tggattgacc aatgtagata cccgtatcgt ctgagccgga cacctgattg aattcgatca
     3541 atccgttggt agagcggatc gggaagatgc catatgcctt gttgttgacg taaactccat
     3601 gatcgatttg atagttatca actcgattca agattacacc attccgcaca taattctgaa
     3661 tcagcaatcc ggtcaatcga aagccattta ttcttgcaga aggaattaac gaccggaccg
     3721 tgatgcccga agtgccgggc aagcccgtcc catccagaat ggcgccgacg cttccctcga
     3781 ttgtgatgtt gtttttcgtg acaaaaacat tctcacggta aacacctggc ggcacacgca
     3841 cagtgtcacc cggactggcg gcatccaccg cggcttgaat ggtcgtggta acaacaatga
     3901 tgtcggattg tgcctccaca gaataggcat ttgtaaacgt gatcgagagt aagaaaagca
     3961 aactcacaat aacagaaaca aatttgtgtt tcataattca tctcctgttt ttgaactgca
     4021 ataggttatg aataaacggt tcgactgaac cgcctatttt aggtgcagca cttcatcagg
     4081 gcgttgttgc agcagaactg tcaaaggcgg ccgggtcgat ccgcagtacc acattctggt
     4141 caagtgcccc aacccagatt gagtcgaatg caacaagcgg aggtccgaga ttccagtagc
     4201 gagtggagag cgatcctgag atggaatttg tcgcggggtc aatcctgtag atcgttccat
     4261 ctccaaaccc tgaagcccag attgcatctg gcccaacgcc aatcccgcca atcgaattgt
     4321 ttaccttaat cgtttccgtt accgcgttcg tcgcaggatc gattcgatat aaggtaccct
     4381 tagcgcgcga ccctacccaa acagagccaa aagcgacttc gagcccaatg aagaaaggat
     4441 ccgaaatact aatggaagtc atcacctggt tcgtcgctgg atcaatccgc cagaccgtac
     4501 caccatccga cgcaccaagt gcccacacag cctctggccc gatttttatc tcaaaaatat
     4561 ctgcggccag aggaattaca gcgcgttcct gcagagtagc tacatcgtaa cgaacaacct
     4621 tcgaacccat tgggctcgta acccagaggt cgttatcgtc cgcagtgaca ccgcaagccg
     4681 taggtagttc aatagaccct acgaccgccc cgctagctgg gtcaattttg gtgacccgac
     4741 cttcgtcaag gcttggggag ccatccgaat gagcctgggc cgcccataca aaaccgccgc
     4801 cgctggccac gctgcaatgt accggtgcgt cttcgagcct ggtaacttcc ttgttctcca
     4861 atgggtcgat acgagtcacg atatcttcac gatgtgactc gacccatatg gaatcagagg
     4921 tggctgcgag cgtagtgggt cccatggcca cgggcacgat ggcgagaatt cccggcgtcg
     4981 actccgtctg cgttggtggg atgactgtcg ggataggaga tgcggtggcg agaggtgctt
     5041 cggtcgagac tgacgctgtc ggcggtactg ccatgggagt tggggttggc gcagcgccgc
     5101 atcccgacat caagatggaa aaaacaacaa ggatgatgga tacacgtgtg attacattca
     5161 ttcttaactc ctttcggctt aaagacctgt gcagtaatta acatcggttc ctataatatt
     5221 tttcctctcg ataatacaaa gtcaccgcaa caaaaccgta acagaacagc atcgagtcca
     5281 aattaaacaa aaaacctgct caaggcaggt atgttaaaga gaacttctaa tgtcgtgctc
     5341 ttctcaacag aaatttacta ctccctctca gaaagagcat attcactggc ttgtgtccat
     5401 tccataacat gaccctcggc ccacgcctgg ctgaactttt cttcacctaa cagagcgcgc
     5461 acagtgctca ggttctgttc atgcaccggt gcctcgatgg agggaaccgg atagccaata
     5521 gcctcacgca acgttgcctc cgcgccaaat aaccgggcgg cacgctcgaa cttattctgc
     5581 gcgcatgcca ggtctgcaaa acctctaaga tagtcggcaa cctctgccct ggcacctctt
     5641 tgctgggcca gcattaatcc ctctgcatac attgaggcag cgttatcaaa atctcctttc
     5701 tgttgtgcta aaagccctaa accatgcaaa gcacgcgcga tgcccacctc gtcgccaagt
     5761 tcccggagca tcggcaagct ctcttcgagt agtgcaagcg caccttcgtt ttcacccttg
     5821 taacatgccg taataccaag atacatgagc aatgtggcaa taccgccctt gtcctgtctt
     5881 tctcgaaaca aggtgagact ctcggtgaat gctgacgttg cacgatgata atccgacaaa
     5941 aagagtgcta ccaatccgat atgctggagg gtccaggcag cacgccacga gtttttcaat
     6001 tcgtgaaata atctcagggc ttcctccagc agttctgcag ctcgggcata ctcgccctgt
     6061 tgctccacca ggtttcccaa ccccatcaga gccagcgctc gtgtagatgc cgatgctcca
     6121 ttactagtag taagtgcctg ttccaaccag cgacggcctt cactaaactg cgcacgaaca
     6181 taccagtacc acacgatcaa ccatgcaaag tgcagctttt cctctgcctc tccatgctgc
     6241 atagaccatt caagtgctgc ccgcagatta ccggcttcca gttcaaaccg attcaaccag
     6301 gttatctcta cggcccccca tagacctcgt ggttctgcct gctctgccat ggccagaaag
     6361 taatcgcggt gattggcccg tattctttcc tcttcacgcg agtcgagtaa cctgtcagca
     6421 gcatactggc ggattgtttc caaaacgtca taacgctttt catggcgttg cttctcaaca
     6481 atgatgagcg atttctctac caggcctgac aagcaatcaa gcagctcact ctccttgaaa
     6541 ttatcagctc cacaaattgc tcccgcggca tccaatgtaa atccgcctgc aaacacagca
     6601 agtcgacgaa agagcacacg ctcattttcg ctaagcaaat cgtagctcca gtcgatcagg
     6661 gcgcgaaggg tctgctggcg cggtaaggct gtacgactgc cgcgcgttag caaattgaag
     6721 cgatcatcca gccgcgccga gatttgagct ggggacaaaa ctttgacgcg cgccgcggct
     6781 agttcaattg caaggggaat gccatccagg cgccggcaga tctgtacaac tgacaatgcg
     6841 ttttcagtag tcaactcaaa atccgcgctg accgactggg cacgctcgac aaaaaggcga
     6901 atactttcgt aagacatcag gagctctgtc agcgacagcg tttcagtttc tggcactgac
     6961 aatttgggca ccggccacac cgtttcgcct gtgatgttta gaagttcgcg gctcgtcgcc
     7021 aggaggcaca agttgctgca ttgtgtcaag agatgatttg caagctgcgc agatgcagtg
     7081 atgagatgtt cgcagttgtc tatgaccaac agtagttgct tttcatcaag aaaccctgca
     7141 agcgtttcga gcaaaggctg gtttggaact tcacgtacgc ctaaggcttt ggcgacgatt
     7201 tgtggcacca atacttcgtc tgtcaatggt gctagatcca cccaccatac gccatcctta
     7261 aattggtcaa tcagatctgt tgctacctga atcgcgagcc tcgtcttacc actaccaccc
     7321 gcgcctgtta aggtgagcaa tcgcgagtga gcgagtaatt tctttacctc gcgcgattcc
     7381 tgcttgcgac cgataaaact tgtgaggggg atgggtagat tggtaatacg cgcggcaaat
     7441 gatggaacgt ctgagggcgt ttgcttgatc cagtgattca gtgcgtttgt ttcaggcgca
     7501 ggctcaatag caaactcctc ttgcaaaacg cgttccgctt tgccgtattg ttccaatgcg
     7561 gcttgacgat ctccacttgc catataacaa aacatcaact gctggtgggc gcgctcattc
     7621 gccgggtctt tctccagaat tttcttcgca atttcaatgg cgcgttcata ctcactttga
     7681 gcacgatacg cctgggtcaa attcagaagt gcactgagat acaactctcg ataatggtcc
     7741 cggaaagcca gaatccaatc atcataaaaa tctgaaagca aatcaccacg atacaatgcg
     7801 atggctgctt cagggtctgt ttgcctctcg aactcacgta tatcgaccca tagcggataa
     7861 tcgggattga tctgaatcaa gtcatgctcg gtaagaaaaa ggttttcacc tagtgctttg
     7921 cgaagtatgg caagtgcatt acgcagtgag gcgcgcgcct gcgcatcgga gaaatcaccc
     7981 cacaatagag ccgcgagttt ttcgcgcgaa tgagtttgag gatgcagtat aagatgggag
     8041 agcagggctt cggctttacg acgcggcagg tgaattagtt caaccgacgc atccgttgaa
     8101 tcgacgtact cgatctgaaa tgaccccaac agatacacgc gatatgatga gcgaaccatc
     8161 acaatcatca tagcacgcgc ggacaagata gtccactaat aagaatcaac atgaccaggc
     8221 ctatcccggt catgttcttc aggcgaaagt caggtactac gatacttaag gcttttgcga
     8281 attcctggaa tagtgtctat tactccttct tcgtccgcac ccaatacgca acaccgatca
     8341 cccccataaa cagcccaccg actgaagcac cgtaggcaaa aaagtttagg aagaatgttg
     8401 attccaggac ttttatcaat atcaaaaagg gaagaacgat ccctaataac atcagtatta
     8461 atcccagacc taccatgaag cgtggagact tgagcatcta gtcgtcactc ttggtttgtg
     8521 tatttccatt tggattccga aacgcatcca aagcctccgg tttatcataa agccagcagg
     8581 ctacaaaatg accaggctca ggttcgaaat cgggcgggaa ttttacgtca cataagcctg
     8641 ccataaagtg tgggcaacgc ggatggaacc ggcacccggt cggagggttg acaagactcg
     8701 gcggttcgcc gggtggtacc tccttgaaag agtcagcgtt acgcgcatcc gggtctgagg
     8761 ttgcggcaag caaggcatgg gtgtaaggat gtaatggatt ttcaagcaga ttgcgcgttt
     8821 cggctttttc gatgagattg ccagcgtaca tgacaaagat gcgcttcgaa aaatatttca
     8881 cagttgaaag atcgtgcgta atgtaaatga ctgacagacc atgtgattgc tgcaaggcgc
     8941 gcatcagttt gaggatttcc acgcgcaccg aagcgtccag catcgaaacg ggttcatctg
     9001 ccacaatcag ctttggctca cgcagcatgg cacgggcaat tacgattcgt tgttgttggc
     9061 caccactcaa catgtgcggg aacttttgta aaaagtcatc cacgggatgc atttttactt
     9121 cggtcatcac tttgtgaata cgggcaagcc gttcctctgg atccttcatt ccactgatca
     9181 acagaggttc ttccaaaatc cgttgaacgt tcataaaggg cggtagagcg ccataagggt
     9241 cctgctgaac atagccaact ttaaagcgat accgctgcac ctcattcccc ttcaaatcgg
     9301 ataaattttc gccatcaaag accacatcgc cgctggttgg gatattaagt ccgagaattg
     9361 ttttcatcag gctcgattta ccgcatcctg actcaccgac gacagctact gtttcaccct
     9421 cctccatgtt aaagctgacg ccatccaccg ccttgacata gccggcgtgg ccgaatccga
     9481 aacggcgtaa ttcataccac acatgcaggt cttgaatcga taacaatgtc tcggcgtctt
     9541 ttgtttctga agcactcata gttacctctt gcaattccgt tttcataatt ttacctgttc
     9601 ccttttcctg ttattttggc gaacggaatt tgctgacacc ccagtgaaag acacccagcc
     9661 tcataaaaaa tactccgaga cccatcggac ctgtccaaaa aaaggtgata aataaatctt
     9721 ccatcacttc tccattaatt tcgtttttct gttgctgagc caaaacccgt ctgcaaccct
     9781 ttcagacact ccatcattcc gaggtggcgg cagtcgtggg tcaagaagac gagcaggttc
     9841 aatcgaattc gttcgacctt ctttctcagt tcttcatcca gatgctcaaa gtcggcgtac
     9901 tttgtatcca gttgttccac gaattcttca atcgcattat atgaatttcg caaataatcc
     9961 agcaattcat cctttggcgg gatgggcagg ctggctgcca cttcatcgct caaaccggtt
    10021 cctactgttt catgggcttc tatcggaaac tcccatttcc tggcgagtcc attccgagac
    10081 cagatttcgc cactattttc aacttcaagt tccaaaccgg gaaaaaactc gaggatcgcg
    10141 gctttgagat aatctgattc gcgcgccagg tgccagaggt ggaatccgat cgatgtgcta
    10201 tagccttcgg gtttccaggt gagttgttcg tcagtcaggc tctcggcaag gttgagaacg
    10261 actttgtgga tatccttgaa atggtaaaga atgtcgtaga gagcgaggga ggacatatgg
    10321 ctctttaagc agatggtttg cgcagccgta acccgaccag aaaaagaacc agactcatga
    10381 caaaccaaag gatcatcaga accagcatcc cgctatcata tacttgtcca ttgtagctgt
    10441 cagcccattc ccacgacgcg aaaccgaagg aaacccagga ggtaactgta gcaatgaacc
    10501 agaagaagct gataaatttt tccattgtag actccgttaa gcgtgcagcc agcacttaac
    10561 cttgcgtccg ttttttccga acatgggggg ttcttcgtcg cacttctcaa atcggaaagg
    10621 gcagcgcgct gcgaaacgac agccttttgg agggttcaac aggctgggcg gttgaccggt
    10681 aataaacatc gggtctgagt cgcttctcag acgcggcacg ctggccatca gcatctgcgc
    10741 gtacggatga agcggcgcag ggaagaaatc gcgcgcgtgc cccacttcca cgatctgacc
    10801 tgcatacatc accgcgactt catctgcaag ttcgctggag gtggcaatat catgggtaat
    10861 caggatgaat gatgtaccca gttcctgctt gatgcgtttg agaacattca tgatgttggc
    10921 ctgcgtgagc aggtcgaggg cagaggtggg ttcatccaaa acgatcaaag ccggagaggt
    10981 cacgagtgcc attgccaggg ccacacgctg acgcattcca ccgctcagct caaacggata
    11041 acgctccaca aaatcgcgcg gcacgcctac atgttggaac atcttgtgga caagcccaat
    11101 cgcatcggcc cggtttaatc ccaggtgcgt aatggctggt tcagccacct gatcacccac
    11161 tttcaacacg gggttgagcg agttcattgc agcttgaggc acaagggaga tacccaccca
    11221 gcgcacattc ttgcgaaact cttcttcatc gaatttcatc acatccgtgc cctgcaaatg
    11281 aacatggccc gaatatttat ccacgttgcg cggcagaagc ctaagaatgg cttttgccag
    11341 tgagcttttg ccgcagcctg attcacccag gactaccaca gcacgattgg agtccaattc
    11401 aaaattaacg ccatccacgg cctgcacaac gccaccgcgt gtcttgaagt gtaaaacaag
    11461 tttttcaatt tgaagtaatt tatgtccgtt tgaattcatg catccctcaa cttgggattg
    11521 aagacgcggt ccaatgcaaa cccaagcatc gcaaaaccaa gtccagtcac catgagcaaa
    11581 actgtaggct ccaaaatcca atagtaataa ccgcgatata aggcggcatt acggttcgca
    11641 tcttcgatca ttttgcccca ggtcggtaga accgggtccc ccagccccag cacagccaag
    11701 ccagcttcca agaagacgaa cgttggcact gacaatacca atcccggaat gagaagtgga
    11761 atcatacgag gaatcaaata taagaaaaca atacgcgggt tgctggcacc ataggcacgc
    11821 gccgcttcga tataaggaga ttcctttaac tgcacaaaga ttgatcgata gcctttgatg
    11881 gcacccgtga atatgctaag caaaatcgtc gcacccagga tcacccagat actacgtgaa
    11941 tagaaagtgc caatcaggat gagtatcgag aagaatggta aaacgaggtt aatctcagta
    12001 atgcgctgga tcaactcatc cacccaggtt ccataccagg cacccaatgc tgaaatcacc
    12061 atcgtgagaa tcgcagtccc cagggaggcg atcaagccga atgccaatgc cacgggtgcg
    12121 ccccacaaca aaggcaccac aagatcgcgg cgagcatggt ctgtgccagc caatccaaat
    12181 acctgtccat gcagcacaaa ttccacattg atatcggaat tgggttcaaa ggtagtgccg
    12241 gtgatcaata gctgatacgt gcccttttca ggaacattct gctctggcaa cgaaaacaga
    12301 acggggataa tttcctgcgt ccttagcttc gttttcagct tatcatcctg tgaaaatcga
    12361 tacgtgtgtt tttgcccgac agccaggtta gcgatccgaa ccgtctctcc atccggtttc
    12421 aaccattcca acgagatgaa cggctgtttc tccgcaaatg tggaggtgac atatacgatc
    12481 atttcctggg gataatagtc atagttgaag tcgaaggaat agttcatggt gtaggtggtc
    12541 acgtcctgat caccaccctc caccgttttc gccaggctgc catcggaagt tctcactgaa
    12601 aatgactcaa catatttctt ggaagaaaac aggttgaccc atgcgggcgg cgcaaatttg
    12661 ggattctgat accagacctc ctcaccgccg cgccatagac gaatggcctc ccggtacggg
    12721 attttgacca tcgcatagat cgcagtaaac accaacagaa aaacgacaaa cgttccaaca
    12781 acggccgagg gatactggaa caatcgttgg agggaacctc gccacgtatt cataattaat
    12841 tccctcctat cttcactcgt ggatctacca gggcatagat aaagtccagc aggaaaacgg
    12901 taaacgccaa tagataggca ttaatgatcg tcgagcctac aatgattgga atatcatata
    12961 acccaaccgc ctgaaacagc gtcctgccaa gaccgggcca aaggaagata gtctctgtga
    13021 tgatggcacc attccacaac gaaataatga ggagtgcgaa attggtgatg atgttcggca
    13081 aggtgggccg aagaatatac ttttgttcca catcgcgagc ggggagtccc ttggcttttg
    13141 ccatttccac atagtcttca ctggaataga tcaggaaaaa ggttcgatac gtgtatatgc
    13201 tgaggaaaaa cgcactcagg atcaaagcca gactcggcaa gatcaggtgc tttgccaggc
    13261 tgagtgagta atcgaacgta ttcgatggag gaggcgaatc gaccatacct ccaaagggaa
    13321 gcaccctcaa tagggatgca aaaatgagaa ttaaaaagat gccgtagaac catggcggga
    13381 cggcagacgt cggagacagt gcgaccacga tcttgtccca gatgttgcca tagcggcgtg
    13441 aaagattaag agcaagaaaa atgctggaga agaacaggaa caattgcgag atgcccatta
    13501 ataacagcgt ggccggcaat cgttccagaa tgatcagcct gaccgtcttg gaaccagaat
    13561 cgctggtcat attaaaggca cggcccaatt gcagagtcaa cgcattggaa aggaaacgga
    13621 agttccgtat tgcagtgggc acattaagac caagccgttc ctcttctgct gcaatccggt
    13681 cctggatcaa ttttgttcgt acatccgggt ccatttgctg cgcggcaggg ctggcaatca
    13741 ccgagttcgt cacgcgctcg cggatttcgc ctttcatgat ggtatccaca aagccgccca
    13801 tattggcaat cacgatcgtt aaataaattc caattactac agtcacaaat aaggtcaaca
    13861 gtcgtaccac aacataacgt gatacacgta cgaatgtgct ctgatgtctc gcttttttgg
    13921 gggctacgtt ttctgtcggc gtatgggtaa ccactgccat ggcgtcctca ctttcttcaa
    13981 gaccacaaac gaagtgaact acagggttca gaattgtacg acaggggtaa ggacaaagcc
    14041 ttacccctgt cttcattata aacaggtctt acatttatgg gacagctaca aagtcgaatg
    14101 aggtgaacga ggggatggca acagggagtg gtaccacagc aacttcgagc cttgctgatc
    14161 ctgtcggcaa attggatgtg acatctgcag gcagtgtgac ttgatactgg ccatcaccca
    14221 ctgcggtggc atcacccacg ctaacgaccg caccggttgc atcgtataag atgtatttca
    14281 ctcgtgcgat gtcagcctga ggatagggct ggtcattgag attcacgatg gcgtcgaaga
    14341 cagcttcttc accgaccttc acctgacccg gtccatcaag gacggttgtt gcaagcttgg
    14401 gtgagctaaa ttctgaccag cggtctgcca ggtctacaaa ttcagcattg ttcttgagaa
    14461 cggccgtttt ctcggtggtg aaaacctggt ccaaataata cggacctgta ccaacccaga
    14521 agtgatcgtg atcggcgtac cactgctgga ggttggcata gcgttgcgcc gcttcttcag
    14581 ccgtgatgta ctcgcccaag gttggttcaa acggaataag tgattcgctt tctgcctgat
    14641 cgagatactt cttaaggatc tcaaggctgg ggccacccac ccagctcgtg ttttcgatct
    14701 gggcatcatc tgccttcgtt tgagtgtaag cgagttcgcc gttggcttca gccaggttgg
    14761 agatcgcaag gaccggccag ctgttctcgc cagagagacc gaacggtgaa tcgggccaca
    14821 ggggcagaat gttcaattca gcgtctgaat tataagagtc agtatagtat tcaattgtca
    14881 gcgggtcggt ggaggtaatg cgaatacctt tgaaggatgc aagtcccgcg tcaatgctta
    14941 gggcaagcga ttcatcgtaa atagcgcttt cgggcttcgc aggatcaaaa ccctggatga
    15001 tggacatcac aaagtcagcc ggggaaatcg ggctgccatc gtgccacttg acggtatccc
    15061 acaaatcagc ggggtaagta accttgctca tgatattggc agtggcgcca tctgggaatc
    15121 gttccgcggc ggtgacgaat ctctgctctg cggcgttcca atcggcccag gcatcttcgg
    15181 gaacgtccac ctgagccact gcgttcacgg tcaaccaatc caggctctga ttgatcggca
    15241 aaccttcttt atatgtcagt tcagcactgt caatacgctg tgggtaagcc agaccggtat
    15301 atgggtcagc catcaagcca gcgccaccga tgaagttgga agtacccatg gtggtcatgc
    15361 gcatgacatg tgcatcccat acccagttgc ttccagcgac ggtgttccag ggttgcgtga
    15421 agaggtcgtt cgttccgatc ctcatcgttc caccttcctg atccgtgaag cgcaggttgt
    15481 aaggacccac gttcgtcgat tcaggaccag tggcgaggtc gtacgtcacc tggacattat
    15541 cagcatgagg ggcatattgc tgctggtcga tcacccatac aaacagggag tcttccatgg
    15601 acagttcgag agcacgcctc atcatatcgt cgcgctctgc cttgctcgtg aaattaccgt
    15661 tcgccagatc atcgccaagc tcttgaagtt cagggtcaga gacattggaa atgaagggtt
    15721 cgctggcttg aacactggtg ttcaaatagc tctgctggat gttgccgcgt tcatcacgca
    15781 gggaagccaa accagaggga atgtaaccag cggtgtacag atgccactgt ccatcggcgg
    15841 cagttgttcc ctgccagatg gggaaggctt ctgaagaggt cttgtactgt cggtcaaccg
    15901 taaagccaag ggtttccaac tgattggaca cgtaatcacc catcggctgg cgtgtgccgt
    15961 caccgtcaga acggatgagg aatgtcagcg tcaccggcgc gccgttgaac tgccaggttc
    16021 catcggaacc caattctgcg cccatgcccg gcatttcggc gtcaatcact tcctttgcgc
    16081 gatcggggtt ataggcatat ttggattcaa gcgcgcgagc cgtttcgatc aagttggtgt
    16141 attccacaag ctgagtggtg atggggagca atctcggcat ggaaccaccc gcatagattt
    16201 cctggttgat gtagttgcga tcaatcagcc agttcaacgc ttcgcggatc ttccgattgg
    16261 agaatgggtt caggcggctt gtatccgtga acacagccgg gttcaggctg atgccataat
    16321 aaccaccaag ggattgggtg gagggcaagc ctgcatcctg gatggcagga tatacgtctg
    16381 aagcaagatt gaatgagaag aagtcgattg tccccgcctg aatctgagag atagcagaag
    16441 caccgtcaac aacagagata tcgatttcat ccagccatcc gccgcggcgt gttgtgggct
    16501 cggcagttgg cgcctctgta gctggagctt cggtagctgg agcgtcggta gctggagcgt
    16561 cagtcgcaac gactgggggt tcagtctctt gcatggcagg gggctcggtt gcggttgcag
    16621 caggggcaca agctgcaagg atcatactaa gcgccaccag caggctgaga atatagtaag
    16681 gacctttaga attcattttc ttttctctcc ttgaaattgg ataggttcag gaatggatgt
    16741 aaacagatat gaactttgag gggactcacc tccttcggct gccgttcgtt tctttatgtg
    16801 cgccagactt cgatatgcat ggattgcgtg cggcactgtg tagacatgga gggaggcgga
    16861 aaccgggctg cgtagacccg atatccgcct caccatcctt tggattgctt tctgtgagga
    16921 gaattcgtga aagcaaagca gattagtcat gctaacctgc ccacatgata cgtactgcag
    16981 tgaaatcaag catccgcctg tgaatgtgtt tcccctccct caatgtagtg gtatttcatc
    17041 ccccaaaatg gacaaaaaga aactaatcta acgttgcgta agatgtgtaa aaacccctcc
    17101 cgagaggggt atagaaacag attaataaat gaataatttc atcgggtttt gttatactat
    17161 aaagagaaga tgtaggtctg agatgcacaa atattggaaa caggctggat cacgcattct
    17221 aggtctggga ctcgccctcg ctttggcgtc ttgtggaggg gtggagtcgc ctaaaaacgc
    17281 gtctgaatcc atcaaaccgg ggcaggacgt ggctgtttcc acaccgatta acctccccct
    17341 cgccccaagc catgcccctg acgagtcgct tccacagatc tgcaactgcg ttcttcgttt
    17401 tgaccatatc agtatcgagc agggactgtc tcagagctcg gtgcgtgtga tctttcagga
    17461 cagtcgcggt tttctctggt tcggcacaga agatggcctg aaccgttacg atggttacga
    17521 attcaaagct tataaacccg agccaagcaa cccaaatagc atcagcagcg gatggatcaa
    17581 tgcgattgtg gaggaccagg atgggtatct atggataggc acaagcctgg gcggtattaa
    17641 tcgctttgat ccgttaacgg gtgggttcac tcagtaccaa catattgatc ggcaaacgaa
    17701 cactgtcaga gataatcaca tcaatgcgct gctggtagac caggataatc gtctgtggat
    17761 aggaacgtta aatggcttgg atcgcctcga tccagaaacc ggaaactttc atcattatcc
    17821 ctataacgag ctccagccaa aaaccggtga aggcaatgtc aataacccgg acgcccaaac
    17881 aaaccctgac agtatcagat attatcccgg ttccacgtta tcaaacaaac taagcagcaa
    17941 gaatgtcact gctatttatg aggactcatc aggtcgctta tggatcggca cctccgatgc
    18001 aggtctaaat cagttcgatg aagagtcagg cgtatttacg atctaccgaa atattgagga
    18061 tttgccatta tccagcgcca gcaagctaat gatcagcagt aatcgtatta ctgccattac
    18121 ggaaagccca cagggtgatt tatggatcgg gactggcaac gggttgaacc ttcttgaccc
    18181 ggagacaggc gagtttcaaa tttttgtgaa cgacgaaagt gatacgggga gcctggtcag
    18241 taattcgatc aataccctct ccacagacgg cacaggcaat ttatggattg gtactgccaa
    18301 tggattagat cggctgcatg aggattttga gcattttatt cactataaca acgacccgtc
    18361 gtttcaaaac agcctgagca ataattacat catctcgatt tatgaagatc gaggtggtgt
    18421 gctttggttc gggacgtatg gcggaggtgt taataaatat gatcggggac gtgcaaattt
    18481 cgcttattat cgcagtgacc cgaaggaccc gaacagcctg agtgggaatt ttatctttcc
    18541 catccatgtg gataaggaag ggtttgcatg gattggaact tcaggcgacg gattaaaccg
    18601 tttcaactgg cagaccggtg aaaacactcg ttttgttaac gatatggata acctaaatag
    18661 catctcatcc aattcgctgc tttccatcta tgaggatagc gaaggaacga tgtggtttgg
    18721 caccaccaaa agcctggatc ggttcgatcc gaaacaaaag acgttcattc actacaccca
    18781 cgatgaatcc aacccggata gcatcagtgc caatcgagta tatgccatcc acgaagacgg
    18841 caatggggat ttgtggcttg gaacctccat tggcttggac cgcttcaata agaagaccgg
    18901 agtgttcacc cactacaatc cagatcccga agacccagcc tcgttgagcg ggaacaaggt
    18961 caccgccatc cttgaggaca aggaaggcac gttatgggtc ggtacgtctg aaagtggatt
    19021 gaatcgactt aatctcaaca cgggcaggtt cacccattac cgtcacgatt caaaaatcga
    19081 aaacagcatt agtagtgatt caattctctc tgtctatcag gattcgcagg aacggttatg
    19141 gataggaacc gctggcggcg ggttaaatct ctacgatccc gaaaccaacg gctttacctt
    19201 ttatctggaa aaagacggcc ttccaaatgg atttgtttat ggcattttgg aagacgaaaa
    19261 gggtcatcta tggatgagta ccaatttcgg actctcacgc tttgaccctg actcaaaaac
    19321 cttccgaaac ttcagtgcag atgatggctt gcaaagtaac gagttcgatt cgtatgccta
    19381 tgccatggga agagatggag agttttattt tggtggcatt aatggactga ccgtcttcca
    19441 cccgtcgaat atcacggata atccgtatgt gccccagatt tctctgatat cgctttcaca
    19501 ggatgatcag cctgtcacgc tggattcgag cgtggaggcc gcgcaggagg tcactcttga
    19561 atggccacag aattcactcg aattcgagtt tgctgcgttg agttataacc agcctaataa
    19621 aaatcaatac gcctatatgc ttgaaggttt cgatacgaac tggcatttca tcggaacgaa
    19681 acgtagtgga cgttatacca acatccccgg cggcaaatat acgcttctgt tgaaagcaac
    19741 caacagcgat ggcgtgtgga atgagactcc tgtgcaattg gatgtgactg ttattccacc
    19801 ctattggcaa acagtctggt tccgtgcagc gttgagtttg gcagttgtcg cgatcgtcct
    19861 gggcggcgtc aagctgcgca ccaaatcgat tcaggatcgt aaccgtgctc tggagcgatt
    19921 ggttcgtgaa aggacacatg cgctcgaaaa acggggccgc gaactggaag cgctctatca
    19981 ggcagatgaa aagatcctgc gaaacataag tcttaatcag gtctttcaga ctctggtgga
    20041 tgtagcggtc gacctgctgc acgcggaccg gagcgtcgtc tttgcctggg atgaggagca
    20101 gaccaaagtg cggccgcgcg tgagtcatgg ctattccgca cggacgctca aggtgatgga
    20161 atttgcacga ggggaaggga atgttgggca ggtgctggcg acggggaagc ccattatcgc
    20221 ccgcgagctt gagcctgaaa cgatgcgccc ggatgttcgt gaggcgatca tcgccgaagg
    20281 aatcaaatct ttcgtacatc tgcctatcac agttgatcat aaggtggtcg cggtcttcaa
    20341 cgtcgcgttt acccagccaa actttatcgg agaagatacc attcgtttgt tctccgcgct
    20401 gaccgaccgc gcttcgcttt ccattgccaa tatggaattg ttcgagcaga ccaaagatct
    20461 ggcagtgatg gaagagcgaa gccgcctcgc ccgtgacctg catgatagtg ccaagcaaaa
    20521 ggcattcgcc gccctcgccc aactaggcac tgcccgcggc atcttaaatg gaaatggaca
    20581 ctctgtagcg atacatctgg atcaggctga gaatctggtg agcgatgtca tccaggagtt
    20641 gacgttcctt gttcaggaaa tctacccaat cgctgtccag gaaaagggac tggcaactgc
    20701 cttacgtgaa tatatctttg agtgggaaaa tcggaacgac agcacagcaa aactttctat
    20761 tcgcaatgag cgacgtttgc cgctggaagt ggagcaggcg atctatcgcg tgacccagga
    20821 gtctcttgca aatgtagcgc gccatagcga tgccactcgt gtggatatcg cactggttta
    20881 caatgtagat tctgttcagc tttctctttc ggatgacggc tgtggattcg ataccgacgt
    20941 aaagtctcac gggatggggc tacgctccat ccgcgaacgt gtcagcagtg ttcatggtgt
    21001 ggcgcagatt cagagcgccc cagggcatgg cacaaggata cttgttcaga taccattaaa
    21061 ggtccaagaa ggagccagta atggcaaaca atcaacatac gcatagcaat cgtaccagca
    21121 tcctcatcgt ggacgatcat gaggtcgtcc gcaatgggat tcgttcttac atcgaaacag
    21181 taaaagaatt tcaggtagtc ggtgaagcag cttcggggga ggaggcagtg aggctggttt
    21241 ccgagcatat ccccgatatc gttctgctgg acttgatcat gcccggcatg gacggcgtcg
    21301 aaacgactcg gcacatcaag cagatcagcc cgcgtacgca ggttgtggtg ctgacctcct
    21361 accatgaaga tgtgcacatc ttcccggcgt tgaaagcggg cgccatctcc tatattttga
    21421 aagatatgaa aatggagaaa ctagtcgacg cgttatatcg cgccgcgcag aaggaagtga
    21481 cactgcaccc cctggtcgcg gcgcgtgtgt tgcagaacat ccgcagtgat aacagtgaag
    21541 agcagccttt gtttactgaa ctgaccgaac gagaattaga tgtgttgcgc ttaatcgcca
    21601 atggcatgac gaacagccag atcgctgaaa agctggtgat cagcgaatac acggtgaaag
    21661 gtcatgtcag caacatcctg agcaagctgc acctcgcgga tcgcacgcaa gtggctgtct
    21721 acgcctggca aaggggatta gttagccgag aacagatggg aaaaaagtaa cgtgaaataa
    21781 tgtcattgcg agccgccgtt cgttagcggc gtggcaatct tcacattctg tgtccttttt
    21841 gaaccgcaaa gcacgcgaag gccgctaaga atcttaagtt tttctttggg ttcttagcgg
    21901 tctttgcggt taattgattt ccactcgcca gtactctccc ccaccgcctt cgcgcttcat
    21961 tagacccgaa ccaaccaatt cacgccgcag cgtcgctgta tcttcgtaat accctgccag
    22021 aatctcgttc acgcgttttt cgctgtaccg ttttcccgat tcgaaggact ttacaacatg
    22081 gcgcaaaacc gcatcgagct ttttgcgctg agcagggaga gtctttaagc taccatcgcg
    22141 ctttgaataa tctttgatca ccttgttgtc gtatgcatct gcatccacat ccgcaacgac
    22201 ggaagaaagt tcattttgcg agaacatggt acgagctttt tcctgcagca ccgattggtc
    22261 cagttgatac acgctgtaat agctttcggt acgtgaggtc accagttccg cctccgccag
    22321 gcgcgagagg tggtgtgaaa ccgtcgaagc tttgaggttc aataaggcag ccagttcttc
    22381 cacactatag ggtttttccg cgagcaaacc aacgatcttc agccggttcg catctgccag
    22441 cgctttaaag aatgtgacga gttctgtgtt tgaattagtc attcttcata atccggtggt
    22501 cgagtaccgc gaagcgggta tcgagaccac cactaaacaa aagttatctt gtcataagat
    22561 tttcttcaat aacctgttgg tttcgacacc tgcactcccc ggcaccgacc aaccggggcg
    22621 gtgtgcatgc aggtgcaagt gtacgtcccg ccaaaagcgg gcgggactac tcaaccaacg
    22681 gattatttaa ttctccaata gcgcgtccca tcgctctcgc gatcgagaaa accatagtcg
    22741 ataagattac ggcgcagggt agctgtgtcc acatggaagc ggcgcaggat ggtattcact
    22801 tccttctcgg tgtaattcgt gtcaaaagag aacgcatcca gcaggaaatt aagcatgatc
    22861 accattttct tcccttcctg tgggatctgc cgaatggttc catccgcgtt
//
LOCUS       DASTJP010000761         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1433807, whole genome shotgun sequence.
ACCESSION   DASTJP010000761 DASTJP010000000
VERSION     DASTJP010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433807"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..495)
                     /locus_tag="VFQ23_22180"
     CDS             complement(13..495)
                     /locus_tag="VFQ23_22180"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET9909372.1"
                     /translation="MNSSEAAPRIRIQKKNPNGEVVYEYEGDLIERDETSITLKALFT
                     RDDMPFMDVVFKKNDLFIEYYYSDRWYNIFEIHDREDGLIKGWYCNIGQPAVIEDGVV
                     SYVDLALDLWVSANGAQTVLDEDEFENLNLSEELQTGALQGLEELKQLFQTRFASRTA
                     "
     gene            complement(492..1487)
                     /locus_tag="VFQ23_22185"
     CDS             complement(492..1487)
                     /locus_tag="VFQ23_22185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909373.1"
                     /translation="MLEVQNVFKSYEGKQILNDISFAVGAGETVCLLGASGSGKSTLL
                     RIIAGLELPDSGQISFNKLDLTSVPAHLRDFGLVFQDYALFPHLNVYDNVAFGLRMRR
                     TPESEIRERVANGLEMVNLTGFELRQVTELSGGEQQRVALARALAPRPRLLMFDEPLG
                     ALDRTLREDLLNELRTILQQTNIPAIYVTHDQEEAFTIADRLLILHAGKIIRDGTPTE
                     IWADPGSSFVAGFLGLGNIIEGTAAANSKVETELGILVTHCAHKHQVGDRIYVLVRPL
                     EVDNEENVVHGVVKDVIFQQERFKVTLDNGLYFYLNASTKVGEKISVNVKVECLS"
     gene            complement(1487..3112)
                     /locus_tag="VFQ23_22190"
     CDS             complement(1487..3112)
                     /locus_tag="VFQ23_22190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET9909374.1"
                     /translation="MLLWFLPLSFLLVFFFIPVARILLLTFDPSTFTPESLKIVSAAL
                     GFTFYQAALSTLLTLLLGLPAAYLFARYEFRGKSLLRALTAVPFMLPTVVVAAGFNAL
                     LGPRGLLHHLFPFFELPFIGSLWAILIAHVFYNMTIIIRVVGNALSSLDPKIEGAARS
                     LGADSFRVWWNVLLPLLRPSLLASSMLVFLFDFTSFGVILLLGASRFSTLEVEIYLRV
                     LKLPNLPLAALLSLIQLICTLVFSILYTRLVARSTTQLAPRSAQSNLRKPKQTREKIF
                     VAVLIFLLSSFFILPLSAIPLRSFFRLEADRGQRGEVQSGLTTDYYEELFINRRGSVF
                     YVPPIQATINSLGYASATVVLSLLLGFPAAFALARPTRLERVLDPLIMLPLGSSAVML
                     GLGFVISYGAWLTSPLLVPVVHTLVALPFVIRTLQPAIASIPQRLRQAASTLGASPVQ
                     VWKTIDIPILRRATLAAGTFAFTISLGEFGATLLISRPDYPTIPVAIERFLSQPGGLN
                     YGQAMAMATILMTLTTLSILLIERFRLPNAGEF"
BASE COUNT          931 a          788 c          813 g          719 t
ORIGIN      
        1 ggtcttttgt ttttaagcag tacgactggc aaaacgcgtc tgaaacagtt gcttcaattc
       61 ttccagtccc tgtaacgcgc cggtctgtaa ttcctcactc agattgagat tttcaaattc
      121 gtcttcgtct agtaccgttt gagccccatt cgctgaaacc cacaagtcga gagccaaatc
      181 aacatacgac acaaccccgt cttcaattac ggcaggctgg ccgatattgc aataccagcc
      241 ttttatcaat ccatcctcgc ggtcgtggat ttcaaagatg ttataccagc gatcggaata
      301 ataatattca ataaacagat catttttctt gaaaaccaca tccataaatg gcatatcatc
      361 tcgcgtgaat aatgctttga gagtgatgga agtctcgtcg cgctcgatca aatccccttc
      421 atattcataa acgacttcac cgtttggatt ctttttctgt atcctgatcc gcggagccgc
      481 ttcgcttgaa ttcatgagag acattctacc ttaacgttca cagatatctt ttcccccact
      541 ttcgttgatg cattcaaata aaaatagaga ccattgtcca aagtgacttt gaagcgctcc
      601 tgttggaaaa ttacatcttt gactacgccg tgaactacat tttcttcgtt gtcgacctca
      661 agcgggcgca ccaatacata aatcctatct ccaacttgat gtttgtgtgc acagtgtgtg
      721 accaaaattc caagctccgt ttcaaccttt gaattggcag cagcagtccc ttcgataata
      781 ttcccgagcc ccaggaaacc tgctacgaag gaggagcccg gatctgccca gatctcagtt
      841 ggagttccgt cacggataat ttttccagca tgcagaatca acaaacggtc agcaatggta
      901 aacgcttcct cctggtcgtg agtcacataa atggctggga tattggtttg ctgaaggatc
      961 gtccgcaatt cattaagcag atcttcgcgc aacgttcggt ctaacgcccc taacggctca
     1021 tcaaacatca acagccgcgg tcgtggcgcc aacgcacgcg caagtgcgac gcgctgttgt
     1081 tctccgccgg ataattcagt gacttgtctt agctcaaagc ccgtcaggtt gaccatctca
     1141 agcccattgg ctacacgctc tcgaatttca gattcaggtg tccggcgcat ccttaacccg
     1201 aaggcgacat tgtcataaac gttgagatga gggaaaagcg cgtagtcctg aaagaccagg
     1261 ccaaaatccc gaagatgagc tggtacactt gtaagatcga gtttgttgaa cgagatttgc
     1321 ccagagtcag gcaactccaa tccagcgatg atccgcagta aagttgactt gccactgcca
     1381 gacgcaccca gtagacaaac cgtttcccct gccccaacag cgaaggagat gtcattaaga
     1441 atctgcttgc cttcgtacga tttgaaaacg ttctgaactt caagcattag aattctcccg
     1501 cgttcggcaa ccggaatcgt tcaatcaaga gaatgctgag ggtggtaagt gtcatcagga
     1561 tcgtcgccat tgccatggct tgcccgtaat tgagtccacc tggctgtgat agaaatcgct
     1621 cgatagctac cgggatggtc ggataatcag gacgcgagat caaaagcgtc gcaccgaatt
     1681 ccccaaggga aattgtgaaa gcaaacgtcc ccgcggccag agtagcgcgg cgaaggatgg
     1741 gtatatcgat cgttttccag acttgaaccg gcgacgcgcc aagcgttgac gccgcctgtc
     1801 gcaatcgctg tgggatcgac gcgatagcgg gctggagcgt ccgaattaca aacgggagag
     1861 caacgagtgt atgaacaact ggaacaagta aaggtgaggt gagccatgct ccgtacgaaa
     1921 taacaaaccc caaccccagc atgacagctg atgatcctaa tggaagcatg atcaatgggt
     1981 caaggactct ttcaagacga gtgggtcttg ccagagcaaa ggctgcagga aagcccagca
     2041 gaagggaaag gacaacggtc gcgctggcat atccaagtga gttgatagtt gcctgaattg
     2101 gaggaacata aaacactgac ccgcgccgat taataaagag ctcctcgtag taatcggtcg
     2161 tcaggcccga ctgaacctcc ccgcgttgac cacggtcggc ttccaggcga aagaaggagc
     2221 gtaatgggat ggcagagaga ggcaggataa agaaagacga tagaagaaat ataagaacag
     2281 cgacgaatat tttctctctg gtctgttttg gttttcgtag gttggattgt gcagaacgag
     2341 gcgcaagttg agtggtggaa cgggccacca gccgtgtgta aagaattgaa aataccaaag
     2401 tgcatatcaa ttgaatcagt gaaagcaaag cagccagagg tagattggga agttttagaa
     2461 ccctcaaata aatttccact tcaagcgtcg aaaaccgcga ggcacccaat aacaagatga
     2521 caccaaaact tgtaaagtcg aagagaaaga ccagcatgga agaagccagc agggatggac
     2581 gcagcaaagg caagaggaca ttccaccaga cgcggaagga gtctgcacca agggaacgtg
     2641 cggctccttc tatttttggg tcgaggctgg agagagcatt accgacgacg cggataataa
     2701 tcgtcatatt ataaaaaaca tgagcaatca aaattgccca aagacttccg atgaatggca
     2761 gctcgaagaa tggaaacagg tgatgaagca aaccgcgcgg cccgagcaat gcattgaaac
     2821 ctgcggcaac aaccacggtc ggaagcataa aagggacggc tgtcaacgcc cgcagaaggg
     2881 atttgcctcg aaactcgtag cgcgcgaaca gatatgcggc gggaagcccg agaagcaagg
     2941 tgagcagggt cgaaagcgcg gcctgataga acgtgaagcc cagggcggca cttacgattt
     3001 tgaggctttc tggcgtgaag gtagaaggat caaacgtcag taaaaggatg cgtgcaaccg
     3061 ggatgaagaa aaatacaagt aagaaggaaa ggggcaagaa ccacaaaagc aaacgagaac
     3121 tagacagaga tgtcttcctc aacgcggctg ttcccggatt tccctgtcta cttgtttcca
     3181 tatttacttg aggacgatat ctgtccactc ttgaatccag cggtcacggt tggctgcgat
     3241 ctcgtcaggc g
//
LOCUS       DASTJP010000762         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_284222, whole genome shotgun sequence.
ACCESSION   DASTJP010000762 DASTJP010000000
VERSION     DASTJP010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284222"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..1586
                     /locus_tag="VFQ23_22195"
     CDS             420..1586
                     /locus_tag="VFQ23_22195"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET9909375.1"
                     /translation="MTEFFQYDEMTWDAVAALPRDIPLVLPLGSGYDLDMLASQLGDP
                     ARVGLLPPFPFGWRGSGLEIPESVFFPYIANLLDSLRDDGFSRVYCLAPSGIDPQSSF
                     ILNRSAFFLRLPHSTHLQTNPFWPPDSERGKVILFPIGHTEQHGFHLPLSTDNLIINA
                     IAQGTGALVPTRSFAMPLMPYGVSTHRSSFAGTMNAGGRAFEDFWLAVIDVLVQRGFD
                     RFYLMSGHGGNCSFLVTIVKYAGERHRRIFCATAWLHTSGRIGSEVIPKYRTSKIGGM
                     GHAGELETSYVLHLRPDLCHMERVVDETDFVATPDYFMDWLEGGSLVANPPWDDDTRT
                     GAYGAGSHGTAEKGRLWLEAAIQEKADHVEQIHEQHQRREKRRNEGYGLWGKLK"
     gene            complement(1609..2037)
                     /locus_tag="VFQ23_22200"
     CDS             complement(1609..2037)
                     /locus_tag="VFQ23_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909376.1"
                     /translation="MQDWLHFTDSRIALALQYPKVTPQGNLVELDENHEQDTVRVHFR
                     STGSREVYFEITKYQDLSPQMEYEQHKENLERRPEKFVLTDFREIGWMSQPAYEYSLR
                     WSQGSRVVRLIEFENSTYRVLYDPFSPLNVQILLTLRWTY"
     gene            complement(2049..2591)
                     /locus_tag="VFQ23_22205"
     CDS             complement(2049..2591)
                     /locus_tag="VFQ23_22205"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9909377.1"
                     /translation="MNKIVFITGAPASGKSTIARRVAEHFSKSLHIQVDQLREMMVTG
                     AEMPDHNWTDEAARQFQRARSTAAYMAKLYAEEGVDVFIDDVCVPENFPDYYKSLFTD
                     AAVQRVLLMPTPDALIQRMEKRAGPFDKFLINFLPWFYSYLEPMPKTGWIVLDSSDWT
                     IEQTVDELLDRIHASPANVD"
     gene            complement(2684..>2914)
                     /locus_tag="VFQ23_22210"
     CDS             complement(2684..>2914)
                     /locus_tag="VFQ23_22210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909378.1"
                     /translation="VVYPEANPAAWSIFLRDVTRVCQYFASQGWRRDARKVAAELWTS
                     HGHRIVKEVDPSHLDGDKQEDRRLWEKQKVEK"
BASE COUNT          723 a          729 c          692 g          770 t
ORIGIN      
        1 cagggttcaa agtgtccgga ctccccgccc accccactcg caagaccgag ggctgagatg
       61 cagcgattcc tccacaagac gacagtacta gtgcaatcac tgtgaccaaa acgagactca
      121 aataactctt tttcatcctg ctcctccagc aaagttgaaa tgtcgcgagc taaacctcgc
      181 gccaaataaa aaaaattgca tcacggtgat tgtagccgcg atgcagaaac taccagatgt
      241 acttctgtgt tgtgagtagc gtacctgctg atgattcagt catgtacatt caatgttatt
      301 ttactttagt ttgtaggttg ggaacaggcg gttatttgtt tggtgattaa tagtagtaga
      361 aaaacagaag taagatattg gctatgcgca aatttatttt atatcaagga gaatgcttca
      421 tgactgaatt ttttcaatac gatgaaatga cctgggatgc agtcgcagct cttccacgag
      481 atataccgtt ggtcctgcca ctcggctctg gttacgacct ggacatgctc gccagccagt
      541 taggtgaccc tgcccgcgtc ggactcctgc ccccgttccc gtttggatgg cgcggcagtg
      601 ggctcgaaat tcctgaatct gttttctttc catacattgc aaacttatta gatagcctgc
      661 gagacgatgg tttctcgcgt gtatattgtc tcgcgccttc cgggatcgat cctcaatcgt
      721 ctttcattct aaatcgctcc gcattttttc tcaggcttcc tcattcaacc catttacaga
      781 caaatccgtt ttggccaccc gactccgaac ggggcaaagt gatcctattt ccaattgggc
      841 atacagagca acacggattt catctccccc tctccaccga taaccttatt atcaatgcca
      901 ttgcccaggg cacaggcgcc ctagtgccta cacgcagttt tgctatgcca ttgatgccat
      961 acggagttag tacccaccgg tcttcgttcg cgggcacaat gaacgcgggc gggcgcgcct
     1021 ttgaagattt ctggctggcg gtgattgatg ttctcgtcca gcgaggcttt gaccgttttt
     1081 atcttatgag cgggcatggt ggcaattgtt ctttcctggt caccatcgtc aaatatgccg
     1141 gcgagcgaca ccgtcgaatc ttttgcgcga ccgcctggct tcacacctca ggcagaatcg
     1201 gctcggaggt cattccaaaa tatcgaacct cgaagatcgg agggatgggc catgccgggg
     1261 aactcgaaac gtcctatgtg ctgcatctcc gcccagatct gtgccacatg gaacgtgtgg
     1321 tggatgagac agatttcgtt gccacgccag attattttat ggattggctc gaaggcggtt
     1381 cgctggttgc gaatccgccg tgggatgacg acacaagaac aggagcctac ggcgcgggaa
     1441 gccatggtac ggcagagaaa ggccgcctat ggctggaggc tgctatccag gaaaaagccg
     1501 atcacgtgga acaaatccat gagcagcatc agcgtcgcga gaagcgcagg aacgaaggct
     1561 atggtctatg gggaaagctc aaatagcaag ggagatcaaa ccgttttatc aatatgtcca
     1621 ccgcaatgtt aataagattt gaacgttgag cggagaaaat ggatcataaa gaacgcgata
     1681 tgtcgaattt tcaaattcaa tcagcctgac gactcgtgat ccctgactcc acctgagcga
     1741 atactcatag gcaggctgag acatccagcc gatctctcta aaatcggtga ggacaaattt
     1801 ttccggccgt cgctcaagat tctctttatg ttgttcatac tccatctgcg gggatagatc
     1861 ttgatatttg gtaatttcaa aatacacttc gcggctacct gttgatctga aatgcacgcg
     1921 aacagtgtct tgttcgtgat tttcgtctaa ttcaacaaga tttccctgag gcgttacctt
     1981 tggatactga agagccaggg cgattctaga gtctgtgaag tgcagccaat cctgcatact
     2041 tgtaatttct aatcgacgtt tgctggtgaa gcatggatgc ggtccagtag ttcatcaacc
     2101 gtctgctcaa ttgtccaatc gctcgaatcc aaaacaatcc aacctgtttt tggcatcggc
     2161 tcaaggtaac tataaaacca gggtaggaaa ttgatcagaa atttatcaaa tggacccgcg
     2221 cgtttttcca ttcgttggat cagggcatca ggtgtaggca tcaacaagac tcgctgcacc
     2281 gcagcgtctg tgaaaagtga cttgtaataa tcaggaaagt tttctggaac acatacatca
     2341 tcaatgaaaa catccacacc ttcttccgcg tacaattttg ccatataagc cgcggtagat
     2401 cgcgcgcgtt ggaactgccg tgctgcttca tcggtccaat tgtgatcagg catttcagcg
     2461 ccggtaacca tcatttcccg cagttgatcc acttgaatat gaagactttt ggaaaaatgt
     2521 tccgcgactc tacgggctat tgttgattta ccagaggccg gcgcgcctgt tatgaataca
     2581 atcttgttca tcgtagatgt cctttagcag gtttggattc aagcaaattg tgtggcacgc
     2641 cgggtagagt gccagcctga tagatattct atcaggctgg tttttacttt tcaactttct
     2701 gtttttccca caaacgacga tcttcttgtt tatccccatc caaatggctg ggatccactt
     2761 ctttgacaat ccgatgccca tgagacgtcc atagctctgc cgcgacttta cgcgcatcgc
     2821 gccgccagcc ctgcgaggca aaatattgac agacacgcgt cacatcacgc aggaagatgc
     2881 tccatgcggc agggtttgcc tcaggataca caac
//
LOCUS       DASTJP010000763         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_806845, whole genome shotgun sequence.
ACCESSION   DASTJP010000763 DASTJP010000000
VERSION     DASTJP010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806845"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="VFQ23_22215"
     CDS             <1..1793
                     /locus_tag="VFQ23_22215"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9909379.1"
                     /translation="ADENYQITYWNRAAELLYGWRAEETIGKFGPDITKTEFPNENPD
                     EMQRQIREQGFWRGEVTQIKKDGTRFPSEISSLLLRDENGKITGYVSLNHDITERKQA
                     EADLRRREQEFRSLADNIPDIISRFDRQLRHIYINDVIESVAGQPSESFMGKTNLELG
                     MPSAQVRIWRDSLRQVFESGKSMAIEFEFPSVAGLRSFESRLVPEFGPTGDVESVLSI
                     ARDITGRKQAEQQTQLQIQRLKALRAIDVAISSSFNLNLTLSILLDQVIGQLNVNAAS
                     ILLFDSIVRGLEYGASRGFLTSYIREAKVRLGEGYAGKAVLERRTIHIENLMQRAEEL
                     PPAFLKMLQEEGFREYYCVPLIVKGEAKGVLEIFHRSALDTNLEWLDFLETLAGQAAI
                     AIEDSTLFENLQRSNQELFQAYDATIEGWSHALDLRDRETEGHTLRVTELSVDLARHF
                     DFTEEQLIYVRWGALLHDIGKMGVPDSILLKPAKLTDVEWEIMRQHPTFAFEMLSPIT
                     FLKTSVDIPYCHHEKWDGTGYPRVLKGDVIPLAARLFAVVDVWDAVTSDRPYRRAWSK
                     EEAAQYLREQSGKHFDPHVVNVFLNSIARM"
     gene            complement(1834..2610)
                     /locus_tag="VFQ23_22220"
     CDS             complement(1834..2610)
                     /locus_tag="VFQ23_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909380.1"
                     /translation="MQPSTIGGINKLPEDEKRAIYSRYIPKELIQKFNLSDLTNNEDL
                     LKFRFLAGSSDVEMMVYHQKDFPDPILYAHLSDSMNGQIYILLYILNDPASPRFNVDK
                     MPDGRPTQFGIRIRNIEAEKAALQAGLSPGQIRHGLRSLQYALNACEEFMSSLGHDMY
                     YMEPLYYHNAIIFERYGFSYQLGRRQMNEIQAGFGESGQFRKMLDGSNPFRSPKAASS
                     IRLRSWAIHDGILGTPFTNVTMYKRVGISANINTAPGCKW"
     gene            complement(2683..3141)
                     /gene="smpB"
                     /locus_tag="VFQ23_22225"
     CDS             complement(2683..3141)
                     /gene="smpB"
                     /locus_tag="VFQ23_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET9909381.1"
                     /translation="MDENVKVIATNRKASFEYFLLEKFEAGLALQGSEIKSIRAGQIS
                     IQESYVDIENGEQAWLVEAHIAPYEQANRFNHDPRRKRRLLLHKKQIRELWNNVRIKG
                     MTIVPTRVYLKKGRAKIEIALAKGKRAYDKRATIAKRDEARSAERESRVR"
     gene            complement(3186..>3428)
                     /locus_tag="VFQ23_22230"
     CDS             complement(3186..>3428)
                     /locus_tag="VFQ23_22230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909382.1"
                     /translation="LFGGLVFALVNGAYANYMLEHIPAHDRTPHLAWYNIILNTAVLL
                     GSLGGPVFANWLGLSDSLVLFSILRLFAGFAILKWG"
BASE COUNT          886 a          796 c          841 g          905 t
ORIGIN      
        1 ccgcagacga aaactatcaa attacctatt ggaatcgggc tgctgagctg ctatatggtt
       61 ggcgcgctga agaaaccata ggaaagtttg gccctgatat tacaaagact gaattcccaa
      121 acgaaaatcc agatgaaatg cagcggcaaa ttcgtgagca agggttctgg cgtggtgagg
      181 tcacgcagat aaaaaaggat ggaacgcgct tccccagtga aatttcttct ctcttacttc
      241 gtgacgaaaa tggcaagata accggatatg tgagcctcaa tcacgatatc accgaacgca
      301 aacaggctga agcagacttg cgtcgacggg aacaggagtt cagatcactt gccgacaata
      361 ttcccgatat catctctcgc tttgatcgcc agttgcggca catctatatc aatgacgtta
      421 ttgaatcagt agccggccag ccctcggaat cctttatggg caagacgaat cttgaattgg
      481 gaatgccatc agctcaggtt cggatctgga gggacagcct gaggcaggtc ttcgaaagcg
      541 gtaaatccat ggcgattgag tttgagtttc cttccgtggc agggttgcgg agctttgaat
      601 ctcggcttgt cccggagttt ggaccgacag gtgacgtgga atcggttctg agtatcgctc
      661 gtgacattac tggacgaaag caggcagagc agcaaacaca attacaaatc caaaggctga
      721 aagctctgcg tgccattgat gtagcgatta gttcgagttt caatctgaat ctgaccctca
      781 gtatcctgct ggatcaagtc attgggcagc ttaatgtaaa tgcagcatcg atcctgttgt
      841 ttgactcaat tgtcagaggt ttggaatatg gggcgagtcg aggttttctc acttcctata
      901 ttcgtgaagc gaaggttagg ttaggcgaag gctatgctgg taaagcggtc ttagaacgac
      961 gtactatcca catcgaaaat ctgatgcaaa gagccgaaga acttcctccg gcttttctga
     1021 agatgctcca ggaagagggc ttccgggaat actattgtgt ccctctgatc gtcaaaggag
     1081 aagcgaaggg tgttctcgag atctttcatc gcagtgctct cgacacgaat cttgaatggt
     1141 tagattttct tgaaacattg gctgggcaag ccgcgattgc aatagaagat tcaaccttat
     1201 tcgaaaatct gcagcgttcc aatcaggaac tttttcaggc atacgatgct acgatagaag
     1261 gctggtctca cgcgcttgat ttgcgcgatc gggagaccga aggccatact ctacgggtga
     1321 cagagttgag cgttgatctt gcgcgtcact ttgacttcac cgaagagcaa ttgatctacg
     1381 tccggtgggg agccttactg cacgacattg gcaagatggg tgtcccagat agcatcctgc
     1441 tcaaaccagc aaagctgaca gatgtggagt gggagattat gagacaacac cccacgtttg
     1501 catttgaaat gttatcaccg attacgtttc taaaaacttc tgtagatatt ccttattgcc
     1561 accatgaaaa gtgggatgga acgggctatc cgcgcgtcct aaaaggcgat gtaattcctc
     1621 tcgctgcgcg gctctttgca gtggttgatg tttgggatgc agtgaccagc gatcgtccat
     1681 accgaagggc atggagcaaa gaagaagctg ctcagtattt gagagaacaa tcgggaaaac
     1741 acttcgaccc ccatgtggta aacgtcttct taaattcgat tgcccggatg tgaatttgct
     1801 acgagtgctg aactcgtaaa tacttccgcg taactaccac ttgcaacccg gagccgtatt
     1861 tatgtttgcc gatatcccca cgcgcttgta cattgtgaca ttggtaaacg gcgtccccag
     1921 aatgccatca tggattgccc aggatcgcag gcgaatgctg cttgcggctt tcggggatcg
     1981 aaatggattc gagccgtcca gcatcttccg gaattgaccc gactctccaa agcctgcctg
     2041 gatttcgttc atttggcgcc tccctaattg atacgaaaac ccgtagcgtt cgaagatgat
     2101 tgcgttatgg tagtagagag gttccatata gtacatgtca tgtccgagac tcgacataaa
     2161 ctcttcacat gcgttcaatg catactggag cgaacgcaat ccgtgacgta tctgtcccgg
     2221 agaaagccca gcttgcaagg ctgctttttc ggcttcaata tttcgaattc gtatgccaaa
     2281 ttgcgtcggt cttccatctg gcattttgtc tacattaaat cggggcgagg cggggtcatt
     2341 caagatataa agcaaaatat agatttgccc gttcatactg tcggacagat gagcatacag
     2401 tatgggatct ggaaaatcct tctggtgata gaccatcatt tcaacatcgc tggagccagc
     2461 aagaaagcga aatttcaaca agtcttcgtt gttggttaag tccgaaaggt taaatttttg
     2521 gattaattct ttcggtatat agcgggaata gatcgcccgt ttctcatctt ccgggagctt
     2581 gttgattcca ccgatggttg aaggttgcat atttttagtt tcttgtttcg tttagatttc
     2641 tggaaaatcc attccaagcc gccagacgaa ttaaaaattg aactatcgaa cccttgactc
     2701 acgttctgca ctgcgcgctt cgtcgcgttt tgcaatcgtg gcacgcttat catatgcccg
     2761 ctttcccttg gcaagtgcaa tttcaatttt ggcgcgaccc ttcttgagat agacacgggt
     2821 cgggacgatc gtcatccctt tgattcgaac attgttccat agttcccgaa tttgcttctt
     2881 atgcaacagg agacgacgct tccgccttgg atcgtgattg aaacggttcg cttgttcata
     2941 gggggcgatg tgtgcctcaa ccagccaggc ctgctcacca ttttcgatat ctacatagga
     3001 ttcttgaatg ctgatctggc ccgcgcgaat ggatttgatc tcgctgccct gtaacgcaag
     3061 acccgcctca aacttttcaa ggagaaaata ttcaaagctt gctttgcgat ttgtagctat
     3121 aacttttacg ttttcatcca tagttggatg ttaccacaat catcctttta cacctgtata
     3181 accgattatc cccacttcaa aatggcgaaa cctgcaaaaa gccgcagtat ggagaataaa
     3241 accaatgaat cagaaagccc aagccagttt gcaaagaccg gcccgcccag agaaccaagc
     3301 agtaccgctg tgttcaaaat gatgttgtac cacgccaaat ggggagtccg gtcatgagcc
     3361 gggatgtgct cgagcatgta gtttgcatat gctccgttta ccagggcaaa gaccagcccg
     3421 ccaaagag
//
LOCUS       DASTJP010000764        16709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_817295, whole genome shotgun sequence.
ACCESSION   DASTJP010000764 DASTJP010000000
VERSION     DASTJP010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16709
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817295"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFQ23_22235"
     CDS             <1..281
                     /locus_tag="VFQ23_22235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909383.1"
                     /translation="NRKKRIQPTLVVVRHFFRSKIGRYVQGPPPPPPTSQREDAIYKQ
                     VLNKIGFVGAIEELIELEREFQPAANRVELLEKINQDWDLDIAGKWKP"
     gene            298..1230
                     /locus_tag="VFQ23_22240"
     CDS             298..1230
                     /locus_tag="VFQ23_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HET9909384.1"
                     /translation="MHITEEHFKKSSDLNRASKALLPIVALESTVLTHGLPRPQNLQL
                     ARDMERAVKDEGATPATIAFLEGHLHVGLNESELEQLANADDALKVGPRDFARVITEG
                     ANGGTTVAGTMLACKHANIKVFATGGIGGVHRESSFDISADLQALATIPMIVVCAGAK
                     AILDLPATLEYLETMSVPVIGYGTNEFPAFYSRESGLDVSARLDTPQEVVDFAKAHWN
                     TGMQSAVLVTNPVPLADAIAKSEMEPIIEKASKEAKDQKIHGQALTPFLLKRISQLTN
                     GKSMKANLSLLLNNARLAAQIARALRASEKRKLA"
     gene            1377..2150
                     /locus_tag="VFQ23_22245"
     CDS             1377..2150
                     /locus_tag="VFQ23_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909385.1"
                     /translation="MNSYRRNAGDGLASLFRDLTRGRRFNRGAAWGLMIIGALLAFEI
                     FNFSTTQFALMDVLGDLSFGQFRWATILAIAFCGIDFAGIARIFTPEQGADEPKEVYY
                     LFGAWLLAAAFNAILTWWGVSVAIVNHASASGGAVISAETMTKAVPVFVAAMILLIRV
                     LIIGTFSLAGDRLFTTDERRAYNSQPRSVQQPAYRPMNNQPVLRSASSLNRPAYTQNQ
                     NTFRPAPKTNVQQTSFTRQEPTYHPVANSAQTGDRSYDA"
     gene            2239..3090
                     /locus_tag="VFQ23_22250"
     CDS             2239..3090
                     /locus_tag="VFQ23_22250"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9909386.1"
                     /translation="MPRARFQNQDDWQTAAAYETGSGCLSFYMLPVLAVFVIAGFLTV
                     LALNSPISVAAPIYNSALQTPVAQVNNTDPAAQVLAPAVDNPIVPSPDSALSITQPEV
                     TNPLWNFSVDNSTSSSGLSPIFTPEIQHWGDDIIRWANAASVDPNLAAVVMQIESCGD
                     PQALSRSGAMGLFQVMPFHFHRGENGFNPETNALRGMNYLSRSLSTGGGNARLALAGY
                     NGGIGVISRSEWSWPAETKRYVLYGAPIYEDARGGATSSPMLAEWYRKYGAGLCRQAN
                     ERLNLAD"
     gene            complement(3110..4480)
                     /locus_tag="VFQ23_22255"
     CDS             complement(3110..4480)
                     /locus_tag="VFQ23_22255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909387.1"
                     /translation="MSLRLRLTLLYATLMGAILLVIGAAVIMLVRVLLLNGIDDNLDS
                     VQEAIIENITITPIGEVEIDYVKAEVTSDVYVQVWGVDGEIQTSFGEFENFDNNVLDQ
                     ASLKNVHPIYRDLRGEDWHIRVLTVPLEQNNHIIAVLQVAASLNVVDKTRADLLDVLS
                     VIWIVAILFLAFLVWLTLGQTLKPLEAITEAAEQINRADDLSRRIPYDGTDNDEIGNL
                     VESFNQTLERLEALFTSQQRLLADVSHELRTPLTVIKGNVDLMRRMKSLDDESLTSID
                     QEAGRLTRLVGGLLLLAQAESGQMALALKKVELDLLLTEVFQEMMILAGNKVRVHLNE
                     IDQVYVNGDRDRLKQVLINLVANAIQYTPQGGDVFLSLEQIKDQARIICRDTGPGIPA
                     EDLPHIFERFYRAEKSRTRGKTTSFGLGLSIANWIVERHGGRIEVNSREGMGTTFAIW
                     LPVWSG"
     gene            complement(4524..5201)
                     /locus_tag="VFQ23_22260"
     CDS             complement(4524..5201)
                     /locus_tag="VFQ23_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909388.1"
                     /translation="MNERILIIEDDQQILKLLQRGLAYEGYTVDTATDGRMGLILARD
                     HTPDLVILDWMLPGMDGLEVCHRLRTGGSIPILMLTAKDTVQDRIQGLDAGADDYMIK
                     PFNLDELLARVRALLRRTQPERVPVLKFADLTLDTGSRQASRGNRSISLTAKEYELLE
                     LFLRHPKQVLTREVIFDRVWGYDFGGESNVLEVYIRYLRQKLEGEGEIRLIHTVRGVG
                     YVMRENP"
     gene            complement(5292..6116)
                     /locus_tag="VFQ23_22265"
     CDS             complement(5292..6116)
                     /locus_tag="VFQ23_22265"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9909389.1"
                     /translation="MLQKMSEYPKFESNDVRILGKRTAEDIESQLLVIQTPFGYRRVA
                     ELFRPAVEGSFAAILYSHWYEPGSPDSNRSQFVEEAKELARAGAVSLLIETLWSDPDF
                     FLKRTQEEDMQNSLEEVVNTRRAMDLLLSQPDTDAQRFAYVGHDFGGMYGVLAGSLDQ
                     RPSHYVIMAATPRFPDWYLYSPKLEGEAREAFISQMTEIDPITHVPNLSPAEIIFQVG
                     TDDPHVPKERAEEFFAAAKTPKEMKWYEAGHGLNEAATLDRTAWLKEKLNLKLNEN"
     gene            6603..7874
                     /gene="serS"
                     /locus_tag="VFQ23_22270"
     CDS             6603..7874
                     /gene="serS"
                     /locus_tag="VFQ23_22270"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET9909390.1"
                     /translation="MLDLNLIREKPDLLRTALTNRQLESTSVDNILRLDEKRRILLTE
                     VEKLKAERNTVSKEIGKMKDAAEREQKIAAMREVGDRISALDKEVAEVEAELNSFVAA
                     LPNIPDERTPIGTSEEENVVLRTVGQLPEFDFEPKAHWDLGPALGILDFERGAKITGS
                     RFYVLSGAGARLQRALIAFMLDLHIRQGYTEKYLPFMVKYDTVFGAGQLPKFADNLYK
                     DHEEELYFVPTAEVPLTGLHMEEILDEAALPLLYTGYTPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYMYAKPEESDALLEKMREDAEATCAALGFTYRVKQLCTGDLGFGSAITYD
                     IEVWAPGCNEWLEVSSVSNVRDFQARRANIKYRPADGGKARLLHTLNGSGLGLPRTLI
                     AVMEQYQQADGSIVIPEVLRPWMGGVEIIKP"
     gene            7933..8451
                     /locus_tag="VFQ23_22275"
     CDS             7933..8451
                     /locus_tag="VFQ23_22275"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HET9909391.1"
                     /translation="MSPEFTQLLLVVLDLLTLAVMLIGLVGLIMPVFPGLVVMWLGAL
                     VYALVQNGAGAMTGWDWFLFALITILMIAGSIVDNIIIARKMRDHFIPWSSILFAFGA
                     SIVASIFFTPIIGLAAAPFALFLAELRRLKNHDEAITSTKAYMIGWGWAFGIRVLIGF
                     VMIGFWMVWAWL"
     gene            complement(8501..8965)
                     /locus_tag="VFQ23_22280"
     CDS             complement(8501..8965)
                     /locus_tag="VFQ23_22280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909392.1"
                     /translation="MSDWKRITREISIENLLPEMVTAINKHIEQYNLGPILSEAVMCI
                     QTDSEKVKKGLFGKAETVHTGAVITPRWLIWATSGSKTVTTVLSAQLNDIVVQDYTQT
                     EFMKLIPDSGMNVSGKFTDVSEDSTAFIGLDDGIAGNKFKETVIKAVQDSKK"
     gene            complement(8982..10262)
                     /locus_tag="VFQ23_22285"
     CDS             complement(8982..10262)
                     /locus_tag="VFQ23_22285"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9909393.1"
                     /translation="MSRFLRFFVHVGLILTAGCASIEQFLVTPTAPPPTSPTPAPDIA
                     TPDGTTSPDANATPTIPSTTQPQILRIWLPSRFDPNEATSAANMLKQRLADFEAEHPG
                     LELDVRIKSDENELDIVNALSVTSKAAPSALPDLLALSRPALESASLQGLLHPMDGLS
                     ATLDDPNWYAYARQLAHIQNTGYGLPFAGDTLILAYHSEVERINSWNDIVASENPLVF
                     TAGDPKALVALSLYVSANGELVNAQGQPTLNAEVLTRVLTLISDGVLAGVFAPSLGNV
                     VSDEQVLQAYLGERANMAIFWASHYRPPEGQFTIPLPGLEATSFSLGTGWVWALAGSN
                     VENQQLAADLADFLVADDFLRDWTTASGYLPTRPSSVDTENTTMTAILESTQLIPSND
                     VLLVLGPIMQDAVNRVLAGEKPEVVAESVINQFP"
     gene            complement(10262..11266)
                     /locus_tag="VFQ23_22290"
     CDS             complement(10262..11266)
                     /locus_tag="VFQ23_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET9909394.1"
                     /translation="MKNLLKQLTETFGPSGYEENVRQLVLSEVKPLADETRVDALGNL
                     IVHKRPSRQTKDAKRIMIAAHMDEIGLIVSHVDENGFVRFSSIGGVFGRYILGGRVQF
                     LNGTQGVIGYDRFDRVNEMPPLDKIYVDVGASSKKDCPVKVGDVAAFDRPFIEMGNRL
                     VSKSMDDRVGVLVAIEALRALKSTLHDVYFVFTTQEEVGVRGAKTSAFGIDPDIGVAI
                     DVTHSADTPNAAKMEMGLGKGPCIKIQDPGMISDPRVVEWMIRAAEKNKIPYQREVLL
                     FGTTDAQAMQLARAGVPSGCISIPTRYIHSPSEMVDYSDIQNSVRLLTAVLNTKIDLG
                     "
     gene            complement(11330..12034)
                     /locus_tag="VFQ23_22295"
     CDS             complement(11330..12034)
                     /locus_tag="VFQ23_22295"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HET9909395.1"
                     /translation="MKGLIALVGSGEYLPVMEEVDRYLLTSLNVNTPQVVCIPTAAGQ
                     EGDESVNRWSRMGVDHFRRLGADVQALPIINKKTADDEQYEAILAGADLIYFSGGNPQ
                     YLFETLNGSRAWNAMQKAWMNGAVYAGCSAGAMILSKRIPSFRLAGTLEGFGIVPAKF
                     IIPHFDAIPGIWKPLVMALKGQLKKGECMIGVDENTALVGELGGEWKVMGKGKVHLFR
                     NKENSVYENGQTLSLD"
     gene            complement(12202..13251)
                     /locus_tag="VFQ23_22300"
     CDS             complement(12202..13251)
                     /locus_tag="VFQ23_22300"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9909396.1"
                     /translation="MPTFGTPQLKLLEKLCNVIAVSGDEGEVRKIVIEEVKPYADEVK
                     VDALGNVLATKLGRGRQRTRVMLDAHMDEVGFMIVADEGDGIYRFETVGGIDVRHLVG
                     KQVFVGKERTPGVIGGKPVHLMDGGERTRKVPLDGLRIDLGPAGKAKVSDRAGFATKF
                     RRAGPSILSKSIDDRIGVVTVIELLKHAPSSLDLCAAFTVQEEIGLRGAKVAAQYFNP
                     DLAIAIDSTPANDLPVFDGGENMIYNTKLGLGPAIYIADGSTLHDPRLVGFLQATAES
                     QKIPYQLRQPGGGGTDSGAIQRSLAGIPTVSVSVPHRYTHSPVSIARVDDWKNTLSLL
                     HAALKRITPQLVAQR"
     gene            complement(13282..14358)
                     /locus_tag="VFQ23_22305"
     CDS             complement(13282..14358)
                     /locus_tag="VFQ23_22305"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET9909397.1"
                     /translation="MADILPFLKSLISVSGLSGFEAPVASLIKERWSSLVDEVRVSRV
                     GSLHGLKRGTASSQSKKTLPSIMIATHMDAIGMMVTKITDGFLHITNIGGIDVRVLPG
                     AQVTVHASSRDELPAIIAMPSSKLLPENVGDGVLEISYLLVDTGLTAREVSRKVKVGD
                     LVSFANEPLELAGDVISGHTLDNRASVAALTVCLEELQGKPHRWDVWAVATVQEESSY
                     LGGYSSAFEIRPDLAIAVDGTFAKGPGANGWQTFQLGKGVGICMGPNMHPFLHKKLKE
                     LAERLELPWFLDITPAHSGTDAYPIQTTAEGIPTALVEFPIRYMHTPVESVSVKDIQR
                     AGRLLAEFIVSLEENFVETIKWDD"
     gene            complement(14360..14791)
                     /locus_tag="VFQ23_22310"
     CDS             complement(14360..14791)
                     /locus_tag="VFQ23_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909398.1"
                     /translation="MLETSDARARRRLGLISIALLTIPCYCLGFIAIALAPSPGDLTP
                     TITETPTVSPTSTLSLTPLILTGTPTSTPTFTPTFTSTATPTTSLTATASQTFTTTPT
                     PTFTFTPSLTFTPSPTFTPSVTITPSITLTPSITSFPSATP"
     gene            14859..>16709
                     /locus_tag="VFQ23_22315"
     CDS             14859..>16709
                     /locus_tag="VFQ23_22315"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HET9909399.1"
                     /translation="MIPTYLRISGFLSYRDAVELDFNSFDVACISGHNGAGKSSLLDA
                     ITWSLFGEARGKSNDIINLHPDVRAAEVIFTFMLEQNTYRVQRILPRNKSTVLEFQVQ
                     NGETWKPLTEKTTRDTQARIEQILRLDYDTFVNASFFLQGKADQFTQQNSSKRKDVLG
                     NILGLEIWEHYRIRAAEKRKVIEREVDEIDGRIREINAELSEEVSRKIKLAELEASLR
                     QLTSARSAQETVLENIRKNAGLLDEQRKLIAALSAGLERAQTALETLETRLSLKEAER
                     DTYARLVDRAQEIESSYKAWQKSRKELEEWDKVAVKFHEHERERTPLLEQIAVEKARL
                     DEERRTLLIEEESIKDQQTSIDSLKLEIEKAEGLLKETEIKIAERGALEITRNTNRER
                     QAELRVENDTLKVEMNQLKERIDTLKSAKGASCPLCGQALSNQHRKSTLRQLEEDGKE
                     RGDRFRANQKETNDLAGQTSTLESQIAGLLSAENERIRFTSEISQRATRLESLQTLSK
                     EWESAGKKRLKELEKLLENRRYAVDEQKQLARLDKELAKLGYDASTHDEAREKESELR
                     AAEEEFSNLKAASEVSRQIQGEISNLRSEIGNRKSEIINLETDYQTAKKNL"
BASE COUNT         4273 a         4264 c         4153 g         4019 t
ORIGIN      
        1 ggaatcgtaa aaaacgtatt caaccaacgc tggttgttgt aagacatttt tttaggagta
       61 aaattggccg ttatgttcaa ggccctccac ctccccctcc cacgtcccag agagaagatg
      121 ctatctataa gcaagtacta aataagatag gcttcgttgg agccattgaa gaactaatcg
      181 agctggagcg cgaatttcaa ccagcggcaa accgagttga attgctcgaa aaaattaacc
      241 aagactggga tctcgatatc gctggaaaat ggaaacccta atttcttaaa tagaactatg
      301 catataactg aagaacattt caagaagtca tccgacctta atcgagcctc gaaagctctt
      361 ttacctatcg tcgcgcttga gtccacagtc ctcacccacg gtctaccgcg gccacaaaat
      421 ttgcagttag cgcgcgatat ggagcgcgcg gtgaaggacg agggcgcgac tccggcaacg
      481 attgccttcc tcgaggggca tcttcacgtc ggtctgaacg aatcagaact cgaacaactt
      541 gcaaacgcgg atgacgctct caaagtgggt ccacgtgatt ttgcaagagt catcaccgaa
      601 ggtgcaaacg gcggcacaac cgtggctggg acaatgcttg cctgtaaaca tgccaacatc
      661 aaggtctttg ccacaggtgg aatcggcggc gtgcatcgcg agtcgagctt tgacatctcg
      721 gcagatttgc aggccttggc aacgattccg atgattgtag tgtgtgccgg cgcaaaagcc
      781 attctggatt tacctgccac attggagtat ctggaaacaa tgagcgttcc agtgattggc
      841 tatggcacaa atgagttccc ggcattttat tcacgtgaaa gcgggctgga tgtgagcgcc
      901 aggctcgaca ctccgcagga agttgtcgat ttcgcgaaag cacactggaa cacggggatg
      961 cagagcgcgg tcctcgtgac gaaccccgtc cccttggcag atgcaatcgc aaagtccgag
     1021 atggagccga ttattgagaa agcatccaaa gaagcgaaag atcagaaaat ccacgggcaa
     1081 gcgctcactc cctttctgct taaacgtatc agtcaactga ccaacgggaa atcaatgaag
     1141 gctaatcttt cgttgctgct caataatgcc cgattggcgg cacaaatcgc aagggcgctg
     1201 cgcgccagtg aaaaacgaaa gcttgcatga agcaaattga ggtcattttg ctcaaatcca
     1261 tgaaccgttg agcaaggaaa ccaatttcaa aattcatcca tccggaatct tgacagtttt
     1321 agaacatgtg ttctataatc gcctcagttg tcacctatcc gacaaaggag gtcgaaatga
     1381 acagctaccg taggaatgca ggggatggac ttgcctctct cttccgtgac ttaacgagag
     1441 gtcgccgttt taatcgcggt gcagcttggg gtttgatgat cattggtgcg ctattggcat
     1501 ttgagatctt caacttcagc accacacaat ttgcattgat ggatgtactc ggagatcttt
     1561 cctttggaca gttccgctgg gcaaccattc tcgccatcgc cttctgcggc attgacttcg
     1621 ctggcatcgc tcgcatcttt actcccgaac agggagccga tgaacccaag gaagtctatt
     1681 atctgtttgg cgcctggctc ctggccgcgg cattcaacgc catcctcacc tggtggggcg
     1741 tttcggttgc cattgtgaat cacgcaagtg cctcgggcgg ggcagtcatc agtgccgaga
     1801 ctatgaccaa agctgtgccg gtctttgtcg ccgcgatgat cctgttgatc cgcgtgttga
     1861 tcatcgggac attttccctg gcaggcgacc gcttgttcac gacggatgag cgccgagcat
     1921 ataacagcca gcctcgttcg gttcagcagc cagcctaccg gccgatgaac aatcaacccg
     1981 tcctgcgctc cgcctccagc ttaaatcgac ctgcttatac acagaatcaa aatacgtttc
     2041 gacctgcacc aaaaacgaat gtgcaacaaa catcctttac acgtcaggag ccgacctatc
     2101 atcctgtcgc taatagcgca caaacaggag accgttcgta cgacgcttaa ctcgtcggat
     2161 ttagttccgg caccccattc aacttaatcc gaactccttt cgagctcggg tcccttacac
     2221 agaatagagg aaccccatat gccccgtgct cgcttccaaa atcaggacga ctggcaaaca
     2281 gccgcggcct atgaaactgg cagcggctgt ttgtcttttt atatgcttcc agtgcttgcc
     2341 gtctttgtga ttgcaggctt tttaactgtg ctggcattga actcaccgat ttcagtcgcc
     2401 gcgcccattt acaattcagc gcttcagacg cctgtagctc aagtcaataa cactgacccc
     2461 gcggcgcagg ttttagctcc agcagtcgac aatccaatcg ttccgtctcc agattcggcc
     2521 ctttcaatca cgcagcctga agtgaccaat ccgctgtgga atttctcagt tgacaactcg
     2581 acctcctcaa gcggactttc ccccatcttt actccggaaa tccagcattg gggcgatgat
     2641 attattcgat gggcaaacgc ggcatctgta gacccgaatc tggctgctgt tgtcatgcag
     2701 attgaatcgt gtggagatcc ccaggcactg tcgcgttccg gagcgatggg tttatttcag
     2761 gtgatgccat ttcactttca cagaggcgaa aacggattta accccgaaac caatgctcta
     2821 cgaggtatga attatctttc gcgttcactt tcgaccggcg ggggaaacgc gcggctggca
     2881 cttgccgggt ataacggcgg cattggtgtc atttctcgta gcgaatggtc ctggcccgca
     2941 gaaacaaaga gatacgtgct gtatggcgcc ccaatttacg aggacgcacg cggcggcgca
     3001 acctcgagcc ccatgttggc agagtggtat cgtaaatatg gggccggttt atgccgacaa
     3061 gcaaacgaac ggctgaacct ggcggattaa agcaactctg agcatttgct caaccagacc
     3121 acacaggcag ccaaattgcg aaagtcgtcc ccattccttc gcgcgagttc acttcaattc
     3181 tccccccgtg ccgttctaca atccaatttg cgatggaaag acccaggcca aagcttgtgg
     3241 tctttccgcg cgtacgcgac ttttcggcac gataaaaacg ttcaaagatg tggggcaggt
     3301 cttcggctgg gatgccgggt ccggtgtctc gacaaatgat tcgcgcctgg tcttttatct
     3361 gttccaggct gagaaataca tctcctccct gcggggtata ttgaatggca ttcgcgacca
     3421 gattgatcaa tacctgcttg aggcggtccc gatctccatt cacatagacc tgatcgatct
     3481 cattcaaatg cacacggacc ttattacctg ccaatatcat catttcctga aagacttctg
     3541 tcaggagcaa atccagttcc acttttttca gcgccagcgc catttgccca gactctgcct
     3601 gagccagtaa tagcaaacct cctaccaggc gcgtcaaacg acctgcctct tgatcaatac
     3661 tggtcaaaga ctcatcatcc agactcttca tgcgccgcat cagatccaca ttgcccttga
     3721 ttacagtgag cggcgtccgc aattcatggc ttacatctgc taaaagccgt tgttgagagg
     3781 taaacaacgc ctccagtctt tcgagcgtct ggttaaatga ctcaaccagg ttcccgattt
     3841 catcgttatc tgtgccgtca tagggaatac ggcgggaaag gtcgtcagca cggttgatct
     3901 gctcagccgc ctcagtgatg gcttcgagag gtttcagggt ttgccccagc gtcagccaaa
     3961 cgaggaacgc caaaaacagg attgccacaa tccaaatcac tgaaagaacg tccagtagat
     4021 cggcacgagt cttgtctaca acattcaagc tggctgcaac ttgaagtacg gcgatgatat
     4081 gattattttg ttccaatggg acggtcaaca cacggatgtg ccaatcctcc cctcggagat
     4141 cccgataaat gggatgaacg tttttcaggg aagcctggtc gagcacattg ttgtcaaaat
     4201 tctcaaattc gccaaaactt gtttgtatct ccccatccac accccagact tgaacatata
     4261 catctgaggt cacttctgcc ttgacataat caatttctac ttcgccgatc ggggtgatcg
     4321 tgatgttttc gataatggct tcctgcactg aatcaagatt atcatcgatc ccgttcaata
     4381 acaggactct cacgagcata atgacggctg cgccaatcac aagaaggatt gcgcccataa
     4441 gtgtggcata caacagggtg aggcgcaatc gaagagacat gcacgtttaa tttacagttt
     4501 tagatttatc gaatcagcaa acgttacgga ttttctcgca ttacatatcc tacaccgcgc
     4561 acggtatgga tcaaacggat ttctccctcc ccttccaact tctgacgcaa ataacgaata
     4621 tatacctcaa ggacgttact ttcgccgcca aagtcgtagc cccaaacgcg atcaaagatc
     4681 acttcacgcg tcaacacctg cttggggtgc cgcagaaaaa gctcgagcag ctcgtattcc
     4741 ttggcggtca acgaaatcga gcgattccca cgcgacgcct gtctcgaacc tgtatccagt
     4801 gtaagatcgg caaacttcaa aacgggaacg cgttccggtt gggtgcgtcg taacaaagcg
     4861 cgcacgcgcg ccagcaattc atccaggttg aacggcttga tcatatagtc atccgcgccg
     4921 gcatccaatc cctggatacg atcctgaact gtatccttcg cagtcagcat caaaatggga
     4981 attgaaccac ctgtgcgtag gcgatgacag acctccaaac catccatgcc gggcaacatc
     5041 caatccaaga tcacaagatc gggcgtgtga tcgcgcgcca ggatcaaacc catgcgtcca
     5101 tcggttgcag tatctaccgt gtagccttca tatgccaacc ctcgttgaag gagttttaaa
     5161 atttgttggt cgtcttcgat gatcaagatg cgttcattca tagttttgct ccttaccaaa
     5221 atataccaca tttggtatgg atgacattag gggaaattcc ccagaaataa atagaaaagc
     5281 gccatttggc gctaattttc attcaatttc aagttcaact tttctttgag ccatgctgta
     5341 cgatccagcg tagcagcttc gtttaaccca tgccctgctt cgtaccattt catttctttc
     5401 ggcgtcttcg ctgcagcaaa gaattcttcg gcgcgctcct ttggcacatg cggatcatcc
     5461 gttccgacct ggaagattat ttcagcaggt gaaagatttg gaacatgagt gatcgggtca
     5521 atttctgtca tttgactgat gaacgcctcc cgtgcctctc cttcaagttt aggggaatac
     5581 aggtaccaat ctgggaagcg cggcgtggca gccataatca cataatgtga aggacgctga
     5641 tccaggcttc cggccagcac gccatacatc ccgccaaaat catgaccgac atacgcaaag
     5701 cgttgcgcgt ctgtatcagg ttgagagagt agcaaatcca tggcacgccg agtgttgact
     5761 acttcttcca atgaattctg catatcctcc tcctgggtgc gtttgaggaa gaaatcaggg
     5821 tcagaccata atgtttcgat cagcaagctg actgcgcccg cgcgtgccaa ctcttttgct
     5881 tcttcgacaa attgactgcg attcgaatca ggtgagccgg gttcatacca atgagaatat
     5941 agaattgcgg cgaatgaacc ttcgacggcc gggcgaaaca attcggcaac acgtcgatat
     6001 ccaaacggcg tctggatcac tagcaactgg ctttcaatat cttctgcagt tcgtttgcca
     6061 aggatacgca catcgttgga ttcaaacttt ggatactcgc tcattttttg taacatggtt
     6121 tctccattgg aaattaggtc cttcccatta tatggaattc atgctgcttg acacgcttcc
     6181 gcagaagtgt ataatcacat tcaacaaaac agcgtgaagc gaagtaactg tgaaaccgtt
     6241 cagagagccg tcggtcagtg tgagacggcc ggaaacgcag tgaaatccac ttcaccgatg
     6301 ttgctctcgc aggggcaaat atgtagtgac gatttcaatc gtccagcgac tgaccctaaa
     6361 gggtgctacg caaagtcgct actactgaaa gccgtaagag agacgtgtgc gcccgttata
     6421 gcgcgagtct gaggccccct cccggcctcc cctattttcg agaaaatggg ggaggtgctc
     6481 cgaaggagcg gtgggggcaa aagcaggtgg caccacgaga cgccccctcg tcctgcaaat
     6541 ggaccggggc gtattctttt ttctggagcc aggaggcttc aggtgcacac ggaggtgcaa
     6601 ccatgttaga cctaaatctt attcgcgaaa agcccgatct gcttcgcacc gcgctcacga
     6661 accggcagtt ggaatccact tcggtggata acattctgcg gctggatgag aagcggcgaa
     6721 tccttctcac ggaagttgag aagctaaagg cggaacgcaa cacagtatct aaagaaatag
     6781 gcaaaatgaa agatgccgcg gagcgtgagc agaaaattgc tgccatgcgt gaggtcggcg
     6841 atcgaatcag cgcgcttgat aaagaagtgg cagaggtgga agctgaactt aattctttcg
     6901 tcgccgcatt gccgaatata ccggatgaac gtacgcccat tggtacgtct gaagaagaaa
     6961 atgtagtgct tcgtacggtt ggtcaactgc cagagttcga ctttgagcca aaagcccatt
     7021 gggatctcgg tccggcgctg ggcattctcg actttgaacg cggcgcgaag attacagggt
     7081 cgcggtttta tgtattgagt ggcgcagggg cacggttgca gcgcgccctg atcgcattca
     7141 tgctggacct gcatatccga caaggctaca cagaaaaata tcttcccttc atggtgaaat
     7201 acgatacagt tttcggcgcg ggacaattac cgaaattcgc cgataatctt tacaaagatc
     7261 acgaagagga actttacttt gtacccactg ctgaagtccc gctcactggc ttgcacatgg
     7321 aagagattct ggacgaagcc gcgctgcctt tgctttatac aggatacaca ccctgtttcc
     7381 gtcgcgagaa aatgagcgcg ggacgcgacg tgcgcggcat caaacgcgga catcaattcg
     7441 acaaggttga gatgtatatg tacgcgaagc ctgaagagtc agatgcctta cttgaaaaga
     7501 tgcgtgaaga tgccgaagcc acctgcgccg ccctgggttt tacttatcgg gtgaaacagt
     7561 tatgtacggg tgacctcgga ttcggttcag ctatcaccta cgatatcgaa gtatgggctc
     7621 ctggttgcaa tgaatggctt gaagtttcgt ccgtctcaaa cgtgcgcgac tttcaggcac
     7681 gccgtgcgaa tataaagtat cgccccgcgg atggtggcaa agcccgtttg ctacatacct
     7741 tgaacggatc aggactcgga ctcccgcgga cgttgattgc cgttatggag caatatcaac
     7801 aggctgatgg atcgattgta atccccgaag tccttcgacc gtggatgggc ggggtagaga
     7861 taatcaaacc ctaaataaat tagggcagcg atcagtgaaa ggatcgcagg ctgccctaca
     7921 acggatatat gtatgtcgcc tgaatttacc cagttattgc ttgtagttct ggaccttctc
     7981 accctggcag tgatgcttat tggtttggtc ggcttaatca tgcccgtttt cccgggactg
     8041 gtcgttatgt ggctgggtgc ccttgtgtat gcgctggttc aaaacggcgc gggcgcaatg
     8101 accgggtggg attggttttt gtttgcgctg atcacaatcc tgatgattgc aggcagtatt
     8161 gtcgataaca tcatcatcgc acgcaaaatg cgtgatcatt tcatcccatg gagttcaatt
     8221 ctttttgcct ttggagccag cattgtagcc agtattttct ttacaccaat catcggcctg
     8281 gctgccgccc catttgcttt gttccttgca gaactaagaa ggctgaaaaa tcatgacgag
     8341 gccatcacgt ccacaaaagc gtatatgatt ggttggggct gggcgtttgg tataagagtt
     8401 ttgatcggct ttgtgatgat cgggttctgg atggtgtggg cctggttatg actcagattg
     8461 gctaaaagcc atcaggctcc cgtttgggag cctgattttt ttatttcttt gagtcttgta
     8521 ccgctttgat gacagtctct ttgaatttat ttcctgcgat accgtcatcc aacccaataa
     8581 acgcagtgct gtcttctgat acatcggtaa atttacccga cacattcatc cccgaatccg
     8641 gaatcagttt cataaattct gtttgagtgt aatcctgaac tactatatca ttcaattgag
     8701 ccgatagaac agtggttact gttttgcttc cgctcgtcgc ccagatcagc caccgcggag
     8761 tgattacagc ccctgtgtgc accgtttctg ctttgccaaa caagcctttc ttcacttttt
     8821 cagagtcagt ctgaatgcac atgactgcct cggacaatat cgggccaaga ttatattgtt
     8881 caatatgctt gttgatagcg gttaccattt ccggcaacaa gttctcaata gagatttcac
     8941 gggttattcg tttccagtca gacattattc attctctgtt ttcagggaaa ttgattgatc
     9001 acgctttctg ccaccacttc aggcttttcg ccagctaata cgcgattcac cgcatcctgc
     9061 ataataggtc caagcaccag caacacatca ttggatggaa ttagctgtgt ggactcaaga
     9121 attgcagtca ttgtagtatt ctctgtatcc acactggatg gacgggtggg aagatagcct
     9181 gatgccgtcg tccagtcccg gagaaaatca tcggcaacca gaaaatcagc aaggtcagcg
     9241 gccaattgct ggttttccac atttgaccct gccaaagccc agacccagcc tgttccaaga
     9301 gaaaaagagg ttgcttcaag gccgggaagt gggatggtaa attgaccttc aggtgggcga
     9361 taatgcgacg cccaaaaaat tgccatattt gcacgttcac ccagatacgc ctgcaggacc
     9421 tgctcatctg aaactacatt tcccagggaa ggggcaaaaa ccccagcaag gacaccatct
     9481 gagatcaatg tcaatacgcg tgtcaacact tccgcgttca gcgtaggctg accctgcgca
     9541 ttcactaatt ctccatttgc agaaacatac aatgacaaag cgactaatgc ttttgggtca
     9601 cccgccgtaa agaccagggg gttctcactt gccacaatat cattccagga atttatccgt
     9661 tccacttcgg aatgatatgc aaggatcaaa gtgtctccag caaacggcag tccgtatccg
     9721 gtgttttgta tatgcgctaa ctgacgggca taggcatacc agttcgggtc gtccagcgtt
     9781 gctgaaagtc catccatcgg gtggaggagc ccttgcaaag aggcagactc aagcgccggg
     9841 cgcgagagcg caagaaggtc aggcaatgca gaaggcgcag ccttgctagt cacagataat
     9901 gcgttgacta tatccaattc attttcatcg gacttgattc gcacatccag ttcgagtccg
     9961 gggtgctccg cttcaaaatc tgccagccgc tgcttcagca tattggctgc gctggtcgct
    10021 tcgtttggat caaatcgact gggcagccat atgcgcaaga tctgcggttg cgtcgtcgaa
    10081 ggaatcgtcg gtgtagcatt agcatctggc gatgtggtgc catctggcgt ggctatatcg
    10141 ggagctggcg tggggctggt tggaggtggg gcggtaggtg tgacgaggaa ttgctcgatg
    10201 gaggcacatc cggcggtcag tatcaatccc acatgaacaa aaaatcttaa gaagcgtgac
    10261 attatcccag atcgatcttt gtattcagaa cagcagtcaa caacctgacg gagttctgta
    10321 tatcagaata atccaccatt tctgatggcg agtgaatata ccgagtaggt atggagatac
    10381 acccggaggg tactccggca cgggccagtt gcattgcctg cgcatcggtc gtcccaaaca
    10441 ggagcacttc gcgttgatag ggaatcttgt ttttctctgc cgcgcgaatc atccattcaa
    10501 caactcgagg gtcagaaatc atgcccggat cctgaatttt gatgcagggc cctttcccga
    10561 gtcccatttc cattttggca gcattgggcg tgtcggcaga gtgcgtcaca tcgatggcga
    10621 cgccgatatc aggatcgatt ccgaatgccg atgttttggc accgcgtaca ccaacttcct
    10681 cctgggtggt gaagacaaaa tagacatcgt gcagggtgga tttgagagca cgcaaggctt
    10741 cgatggcgac caaaacgcct acgcgatcat ccatagattt cgacacgagt cggtttccca
    10801 tctcaatgaa tgggcggtca aaggcagcca catcacccac cttgacggga caatcctttt
    10861 tactggatgc acccacatcg acataaatct tatcgagcgg gggcatttca ttcacccgat
    10921 caaaacggtc atagcctatg acgccttgcg ttccattcaa aaactggacg cgtccaccca
    10981 aaatgtatcg gccgaaaacg cctccaatgg atgaaaaccg aacaaatcca ttctcgtcca
    11041 catggctgac gatcagtccg atttcatcca tatgcgcggc gatcatgatt cgcttcgcat
    11101 ccttcgtctg gcggctgggg cgtttgtgca cgataagatt gcccaacgca tccactcgcg
    11161 tttcgtcagc caacggcttc acctctgaca ataccagctg ccgtacattc tcttcatagc
    11221 cagagggacc aaaagtttca gtaagttgtt tgaggagatt tttcatgaag agttcccgga
    11281 gacgcatgca caaatctgtg ccgagctatg cccgtgcaag tatgtaacac taatccaaag
    11341 ataatgtttg accattttcg tagacgctgt tttccttatt tctgaacaga tgcactttgc
    11401 cttttcccat caccttccac tcgccgccca gttcgcccac cagggccgta ttttcatcta
    11461 caccgatcat gcattcacct ttcttgagtt ggcctttcag tgccatcaca agaggcttcc
    11521 atatgcccgg aatagcatca aagtgcggaa tgataaattt tgcaggcacg ataccaaatc
    11581 cttcgagggt tcctgccaat cggaaactcg ggattcgctt cgacaaaatc atcgcgccgg
    11641 cagaacagcc tgcatacacc gcaccgttca tccacgcctt ttgcatcgca ttccaggcac
    11701 gtgagccatt cagtgtctca aaaagatatt gagggtttcc gccagaaaag taaattagat
    11761 ctgcgcctgc aagaattgct tcgtactgtt catcatcggc ggttttctta ttaatgatcg
    11821 gcagagcctg aacgtcagcc cccagccgcc tgaagtgatc cacgcccata cgtgaccagc
    11881 gattgacact ttcgtctccc tcctgtccgg ctgcagtagg gatgcacacg acctgcggtg
    11941 tattgacatt caagcttgtc aacaaataac gatctacctc ttccatcact ggcagatatt
    12001 caccagagcc caccagagca attagacctt tcataattta cctatgtttc ccgctggttg
    12061 aggagtcgaa gcccaatgcg tgcgtcgcac acagatcggg agtgcgggtg tcaaaaccag
    12121 caataagaaa tatgaaatca aatgaatgcc gtcccgagat acgacgattg cacgccacca
    12181 aaggatcgcc ttcagagagc atcatcgttg tgcaaccaat tgaggagtga ttcgcttcaa
    12241 tgccgcatgc aataaactca atgtgttctt ccaatcatcc acgcgggcga tgctcaccgg
    12301 agaatgagta tagcggtgcg gtactgaaac ggatactgtt ggtataccag ctaaggaacg
    12361 ctggatggcg cccgaatctg tgccgccgcc tccgggctgg cgcaattgat agggaatctt
    12421 ctgagactca gccgttgcct gcaaaaagcc gaccagacgt gggtcatgaa gcgtcgaacc
    12481 atccgcaata tagatggcag gtcccagacc cagttttgta ttgtagatca tattctcccc
    12541 gccatcgaaa acaggcaggt cattggcagg ggtcgaatca atggcgatcg cgagatcagg
    12601 gttgaaatat tgggcagcta cttttgcacc acgcaagccg atctcctcct ggacagtgaa
    12661 ggccgcacac aggtcgaggc tggagggagc gtgcttgagt agttcgatca cagtcacgac
    12721 gccgattcga tcatcaatag actttgaaag gatcgaaggc cccgcacggc gaaactttgt
    12781 agcaaaaccc gcgcgatcac tgactttggc tttgcctgcc gggcccagat caattcgcag
    12841 cccatccagg ggcacttttc gagtgcgttc cccgccatcc atcagatgaa caggcttacc
    12901 gccaattaca cctggcgttc tctctttccc tacaaatact tgctttccga ccaagtggcg
    12961 tacatcgatg ccgcccacag tttcaaagcg atagatccca tctccttcgt cagcaacgat
    13021 cataaagccg acttcgtcca tatgcgcgtc cagcatgacg cgcgtgcgtt gccgaccgcg
    13081 accaagctta gtagcaagga cgtttcccaa agcatccact ttcacttcgt ccgcgtaggg
    13141 tttgacctct tcaatgacaa tcttgcgaac ttcaccttca tcgccagaga cagcgatcac
    13201 attgcaaagt ttttcaagga gtttgagttg aggggttcca aaggtgggca taaaggtctc
    13261 agagtaactg atgtattttc gtcaatcatc ccatttgatt gtttccacaa aattctcttc
    13321 cagtgaaaca atgaattcag ccagtagacg tccagcgcgt tggatatcct tcacagaaac
    13381 cgattccacc ggggtatgca tatagcgaat gggaaattcc acgagtgccg ttgggatgcc
    13441 ctccgcggtc gtttgaatgg ggtacgcatc tgttccagag tgagcaggtg taatatccaa
    13501 aaaccacggc agttcgagcc gttctgcaag ttcttttagt ttcttatgca gaaaaggatg
    13561 catgttaggt cccatgcata tcccaacacc tttcccaagt tggaaagttt gccagccgtt
    13621 tgcgcccggt ccttttgcaa aagtcccatc aactgcaatt gcgagatcag ggcggatctc
    13681 aaacgctgaa ctatagccgc ccagataact tgattcctcc tgaacggtgg caaccgccca
    13741 cacgtcccaa cggtgaggct tgccctgcaa ttcctcaagg caaacagtga gggccgcaac
    13801 ggaggcgcgg ttatcaaggg tatgcccgga gatcacatcc ccggctaatt ccagaggttc
    13861 attagcaaac gagactaagt ctcctacctt cacttttctg cttacttcac gcgcggtgag
    13921 acctgtatca accagaagat aactaatttc cagtacacca tcccccacat tttcaggcag
    13981 caatttggaa gatggcatcg caattatggc aggcagttca tctctactac tcgcatggac
    14041 cgtcacttgc gcgcccggga gaacgcgaac gtcaattccc ccgatgtttg taatgtgcaa
    14101 gaatccgtcg gtgattttcg tcaccatcat gccgatcgcg tccatgtgcg tggcgatcat
    14161 gatggacggg agcgtctttt tggactgact ggatgccgtg cccctcttca gcccgtgcag
    14221 cgaaccgacg cggctgacac gaacctcatc gacaagtgaa ctccatcgct ccttgatcag
    14281 gctcgccact ggcgcttcaa aaccggacag ccctgaaaca gagatcagcg attttagaaa
    14341 tggaaggata tctgccatgt tatggtgtgg ctgaggggaa ggatgtgatg gaaggagtca
    14401 gtgtaatgga aggagtgatc gtcactgagg gtgtaaacgt gggcgaaggt gtgaacgtca
    14461 gcgacggcgt gaacgtaaaa gtcggtgtcg gcgtggtagt gaacgtttgt gaagcggttg
    14521 cggtaaggct ggtcgtaggc gtcgcagtag atgtgaaggt cggggtgaag gtcggtgtgc
    14581 tggtcggggt tcccgttaaa atcaacggag tcaggctgag ggtggaggtc ggagagacag
    14641 tcggagtttc agtgatcgtg ggcgtcaaat ctcccggact cggcgcaagc gcaattgcga
    14701 tgaaaccaag gcagtagcat ggaattgtga gaagggcgat agagatcaat ccgaggcgac
    14761 gacgagcgcg ggcatctgag gtttccaaca tgatggttag attataatcc atcattattc
    14821 ttgaaatacg ccgcgttcgt tgggcgtgac acgtcactat gattcccact tatcttcgaa
    14881 tttcaggttt tctttcttac cgcgatgcgg ttgaactgga tttcaactca ttcgatgtcg
    14941 cctgtatatc cggtcataac ggtgcaggga aatcatccct tctggatgcc attacatggt
    15001 ctctgttcgg tgaagcgcgc ggtaagagta atgacatcat caatctgcat ccggacgtga
    15061 gagctgcaga agtcattttc acgtttatgc ttgagcaaaa cacctatcgg gtacagcgca
    15121 ttctgccgcg caataaaagc acggtgcttg aatttcaggt tcagaatggc gaaacctgga
    15181 aaccactgac cgagaaaacg actcgagata cccaggcgcg cattgaacag attttgcggc
    15241 tggattatga cacctttgta aacgcatctt tctttttgca gggaaaggca gatcaattta
    15301 cacaacaaaa ctcaagcaaa cggaaggatg tattggggaa tatcctcggt ctggaaatat
    15361 gggagcacta tagaatccgc gcggcggaaa aacgaaaggt tatcgaacgc gaagtcgatg
    15421 aaatcgatgg gcgcattcgt gagatcaacg cagaactgag cgaggaagtt tcacggaaaa
    15481 taaaactggc tgagttggaa gcttctctcc gacaattgac ctctgcccgc tctgcgcagg
    15541 aaacggtgct ggaaaacatt cgtaagaacg ctgggctgtt ggatgaacaa cgaaagttga
    15601 tcgccgcctt atccgccgga ctcgagcgcg ctcaaacggc gcttgagaca ctcgagacgc
    15661 gtctctcttt gaaagaagct gaacgcgaca cgtacgcccg cctggtagat cgcgcacagg
    15721 aaattgagtc atcgtacaaa gcctggcaaa agtcacgcaa ggagttggaa gagtgggata
    15781 aggtcgccgt gaaatttcac gaacatgaaa gagagcgcac accgttgttg gaacagattg
    15841 cggtggaaaa ggcaagattg gatgaagaaa gacgcacctt gttgatcgaa gaagaaagta
    15901 tcaaagatca acaaacatcc attgatagtc tgaagttaga aatagagaag gccgaaggcc
    15961 ttctaaaaga gacagagata aaaattgctg aacgcggcgc tttggaaatt acgagaaata
    16021 caaaccgtga acggcaagcc gagttgagag tagagaacga caccctcaaa gttgagatga
    16081 atcaactcaa ggaacgtatc gacacgctca agtctgctaa aggcgccagc tgtccgttgt
    16141 gtggacaggc attgagcaac caacatcgta aatccacact ccggcaactg gaagaggacg
    16201 gtaaggaaag aggcgaccgg tttcgagcca atcaaaaaga gaccaacgat cttgcaggtc
    16261 agacctcgac gcttgaatct caaattgctg gtcttctttc cgccgaaaac gaacgcatcc
    16321 gtttcactag cgagatttcg caacgcgcca cgcgattgga atcgctacaa acgctttcaa
    16381 aggaatggga gtcggctggc aaaaagagac taaaggagct ggaaaaactt ttggaaaacc
    16441 gccgatacgc ggtggatgaa caaaaacagc tggctcgatt ggataaggaa cttgccaaac
    16501 ttggctatga tgcatccact cacgatgagg ctcgcgaaaa agaaagtgaa ttgagagcgg
    16561 cggaagaaga atttagcaat ctcaaggctg ccagtgaggt gagtagacag attcaaggtg
    16621 agatttccaa tctgcgttct gaaattggga accgcaaatc tgagattatc aatctggaaa
    16681 ctgattatca aaccgcaaaa aagaatctt
//
LOCUS       DASTJP010000765         5585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_565400, whole genome shotgun sequence.
ACCESSION   DASTJP010000765 DASTJP010000000
VERSION     DASTJP010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565400"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="VFQ23_22320"
     CDS             <1..1784
                     /locus_tag="VFQ23_22320"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HET9909400.1"
                     /translation="GQLPFYFPAVLLMTAIALIIKIPIYYLFGLYRRLWIYASTGELR
                     LITIAVTTASILTSAVMAMLIVTGNVFPGMPRSALGIDWLLSLVLIGGSRFALRILAE
                     QSMASGANGKGKRVLIIGAGDAGALVLRELQKSSQLNLTPVGFLDDDPAKQRHSIHGV
                     TVIGAVNDLQSMIELHQIDQVIIAIPSAPGKLVRQVNNICRIKGITSRTMPGIYELIG
                     GRVRVNRLREVEIADLLRREHVHVNDEAVGAALEGKRVLVTGAGGSIGRELCRQIARR
                     NPAQLVLLGHGENSIFEILLELQQDYPNHIFSPVLADVRNAERLASVFEQHKPQVVFH
                     AAAHKHVPLMEANVVEAITNNVIGTRNVVQVALDANVERFVIISTDKAVRPASIYGAT
                     KRLAEMIVLNAAHNSGRAFTVVRFGNVLGSRGSIIPIFKQQIASGGPVTITHPDMYRF
                     FMTIPEAVYLVLHAASMVQGGETFVLDMGLPVRILDLAEDLIRLSGLEPHRDIEISYT
                     GIRPGEKLTEELWDSGTPLKKTSHPDIFCLEADGSLPGLNLAKAIDSLSALTHGADPD
                     AIIKLLDELIPGSSIREIPHSDITSVI"
     gene            1934..2902
                     /locus_tag="VFQ23_22325"
     CDS             1934..2902
                     /locus_tag="VFQ23_22325"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HET9909401.1"
                     /translation="MPTVSLTDWNQFLLQHPNTHLLQSGEWGELKSAFGWKPVRIVSG
                     NAGVQILFRKLPLGFTIGYIPKANVKETLWQEIDFVCKQNRAIFLKLEPDFWNDQQPG
                     TWNLELRTSPHNIQPPRTIVVDIKSSEEEILARMKQKTRYNIRLAEKKGVTVRAWDDL
                     KAFHKMMLVTGGRDGFGVHSFEYYKHAYELLQPKQMGELLVAEYEGKPLAALFVARHG
                     NRAYYLYGASTDEERNRMPTYLLQWDAMKWAKARGCEEYDLWGVPDDDEGTLEANFET
                     RHDGLWGVYRFKRGFGGELKRAVQAMDRVYNPLMYWAYLRFIGNRE"
     gene            2906..4051
                     /locus_tag="VFQ23_22330"
     CDS             2906..4051
                     /locus_tag="VFQ23_22330"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HET9909402.1"
                     /translation="MNRNNWDSLISNFPNPHFLQTYEWGQVKAKYGWSPLYSVWDADG
                     KWKVESDPNLFSSFHSPVAAALILKRQILRNAFAARLSILYVPKGPLLDWSNEPLRNR
                     VLNDLQSFARKQGAIFLKTDPDVVLATGVPDSPDNVNDNGGQVVMSDLKRRGWGYSSD
                     QIQFKNTVLIDLNPSEEELLAHMKQKTRYNIRLAQKKGVSVRVGMSADLALLYRMYAE
                     TSIRDGFVIRDEGYYKTVWNLFMNSADPYCEPLIAEVDHQPVAGIFVFYFAGRAYYVY
                     GMSRDVHREKMPAYLLQWEAMKRAKARGCAVYDLWGAPDIFDESDSMWGVYRFKEGLG
                     GKVVRTLGAWDYAPNRLWYNMYSEIIPRVLDVMRSRGKAKTKQNLGA"
     gene            4195..5160
                     /locus_tag="VFQ23_22335"
     CDS             4195..5160
                     /locus_tag="VFQ23_22335"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HET9909403.1"
                     /translation="MNSIPRLHFAHLPTPIEELPRLSEMLGGPRIFVKRDDQTGLAFG
                     GNKTRKLEFLLAEALEQGAKTLITCGALQSNHCRQTAAAAARFGFECILVMNGEMPEQ
                     LSANLLLDRLFGAEIVLVKDRAAREQALQNTFDQALAAGKKPYLITLGGSNSIGALGY
                     AFAMKEFMEQNVDADWILFGTSSGGTHAGLVLGQRVFDFKGKVLGISIDEPEAWLKRR
                     VSELASDASEKLGERIEFTREDVLANEEYCQAGYGVLTDAEREAVKLFAKYEGLLLDP
                     VYTGRAAAGMIDLMRKGFFKRDETILFWHTGGGPALFAEKYANLI"
     gene            complement(5198..>5585)
                     /gene="eno"
                     /locus_tag="VFQ23_22340"
     CDS             complement(5198..>5585)
                     /gene="eno"
                     /locus_tag="VFQ23_22340"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9909404.1"
                     /translation="LMVQKVGNKCQLVGDDLLVTNAERVRRAIKENAANALLVKVNQI
                     GSLTETIEAVETCQRAGWRAVTSHRSGETEDTTIADIAVALNSGQIKTGAPARSDRVA
                     KYNQLLRIEAELGETAKYAGWGALKS"
BASE COUNT         1386 a         1331 c         1438 g         1430 t
ORIGIN      
        1 taggacagtt gccattttat ttccctgctg tcttgttaat gacagcgatt gctttgataa
       61 taaagattcc catttattat ttatttgggc tctatcgccg cttatggatc tatgccagca
      121 ccggtgaatt gcgattgatc acgattgcag taacaacggc ttcgatattg acctcagccg
      181 tgatggccat gctgatcgtc accggaaatg tcttccccgg gatgcctcgt tccgcgctgg
      241 gtattgattg gcttctttcg cttgtgctca tcggtggttc gcggttcgca ttgcggatcc
      301 tggcagaaca atcgatggca tccggcgcga atggtaaagg gaaacgagtt ctgatcattg
      361 gcgcgggaga tgctggtgcg ctagtcttgc gtgaacttca aaagtcatca cagcttaact
      421 taactccagt tgggttttta gatgatgacc ctgcaaaaca aagacattcc atccatggtg
      481 taacggtgat tggcgcggtg aatgacttgc aatccatgat tgaattacat cagatcgatc
      541 aggtcattat tgcgattccc tctgcgccgg gtaagctggt ccggcaggtt aataatatct
      601 gtcgtatcaa gggaatcaca tcccgtacga tgcccgggat ctatgagctg atcggcggca
      661 gagtgcgcgt caatcgactg cgtgaagtgg agatcgccga tctcttacgc cgtgaacatg
      721 tccatgtcaa cgatgaggca gtcggcgctg cgctcgaggg caagcgtgtg cttgtcacag
      781 gtgcgggggg gtcaatcggg cgtgagttat gtcgtcaaat cgcacgtcga aatcctgcgc
      841 aattggtttt gctgggtcat ggtgaaaaca gcatttttga aattttgctg gaactccagc
      901 aggattaccc aaaccacatt ttttcaccgg ttctcgcgga tgtccgcaat gcggagcggc
      961 ttgcttctgt tttcgagcag cataaacctc aggttgtgtt tcatgccgca gcacacaaac
     1021 atgtcccgtt gatggaagcc aacgtggtgg aagccatcac gaacaatgta attggcactc
     1081 gaaacgtggt ccaggtcgcg ctggatgcaa atgtcgaacg gtttgttatc atatccaccg
     1141 ataaagcagt tcgccccgca agtatttatg gcgcaaccaa aagactggca gagatgatcg
     1201 ttctcaacgc ggcacataac tccgggcggg cgtttaccgt tgtccgtttt ggaaatgtcc
     1261 ttggcagtcg tggcagcatc attcctattt tcaaacaaca gatcgcgagt ggcggcccgg
     1321 tcacaatcac acatccggat atgtaccgct tcttcatgac cattcctgag gcagtctatc
     1381 ttgttctgca tgcagcttcg atggtccaag gcggtgagac gttcgtgctg gatatgggcc
     1441 tacccgtccg cattcttgat ctcgcagaag acctcattcg gctctcgggg ctggaacctc
     1501 atcgtgacat cgaaatttca tatacgggta tccgacccgg tgaaaagctt acggaagaac
     1561 tttgggatag tggaacgcct ctcaaaaaaa catcgcatcc agacattttc tgcctggaag
     1621 cagacggttc tcttccaggt ctcaacctgg ctaaagccat cgactctctc tccgctctta
     1681 cgcatggcgc agacccagat gcaatcatca agctcctgga cgaattgatc cccggttctt
     1741 ccattcggga aataccgcat tctgatataa cttctgttat ctaactgata tataccgttg
     1801 gtcgagtagc cctgcgtgct tttggcaggg cgtaccgaga ccattggtaa tcataaaaat
     1861 actagatttg aatccaagtg gtgatctcga tacgacggac gatcgccgcc cactcgacca
     1921 cctaataaat taaatgccca ccgtctctct caccgactgg aatcaattcc ttcttcagca
     1981 ccccaataca catctcctcc aatccggcga gtggggtgag ttgaaatccg cctttgggtg
     2041 gaagccggtg cggattgtta gcgggaatgc tggcgtacaa atcctctttc gtaaactccc
     2101 attgggcttc acaatcggat atattccgaa agctaatgtc aaggaaaccc tttggcagga
     2161 aatcgatttt gtttgcaaac aaaaccgcgc catcttcctc aaacttgagc cagacttctg
     2221 gaacgatcaa caacctggaa cttggaactt ggaactccgc acttcccctc acaatatcca
     2281 acctccacgt acaattgttg ttgacataaa aagcagtgaa gaagaaattc ttgctcgtat
     2341 gaaacaaaag actcgctaca acattcgcct tgccgagaaa aagggagtga ccgtccgagc
     2401 gtgggatgat ctcaaagcct ttcataaaat gatgctagtt acaggtggac gcgatggctt
     2461 cggcgtccac tcattcgaat attacaaaca cgcctatgaa ttactgcagc caaagcagat
     2521 gggtgaatta ctagtcgctg aatatgaggg gaaaccatta gctgccctat ttgtcgccag
     2581 acatggaaat cgtgcctatt atctctacgg cgcatcgaca gacgaagagc gcaaccgtat
     2641 gccgacttat cttttgcagt gggacgccat gaaatgggca aaagcgcgcg gctgtgaaga
     2701 atatgacctt tggggtgtcc ccgatgacga tgaagggact ctcgaagcga actttgaaac
     2761 ccgacacgat ggcttatggg gcgtctaccg cttcaagcgc ggctttgggg gtgagctcaa
     2821 acgcgcggtg caggctatgg accgagtgta caatccattg atgtattggg cgtatttacg
     2881 atttatcggg aatcgagaat agaaagtgaa tcgtaataac tgggattctt tgatttctaa
     2941 tttccccaat ccccattttc tccaaaccta cgaatgggga caggtgaagg cgaagtatgg
     3001 ttggagtcca ctttattcag tttgggacgc ggacggaaaa tggaaagtgg aaagtgatcc
     3061 gaatctattt tcctctttcc attccccagt cgccgctgct ttgattctga aacgccaaat
     3121 tcttcgcaat gcatttgccg cgcggttatc gattctctac gtgccgaaag gtccattgct
     3181 ggactggtca aatgaacctc tcagaaatcg tgtgctcaat gatttacagt cctttgcaag
     3241 gaaacaaggt gcgatcttct tgaaaacgga tcctgacgtg gtgctggcga cgggagtccc
     3301 tgacagcccg gataacgtca atgataacgg tgggcaggtc gtaatgtccg atttgaagcg
     3361 gagaggctgg ggatattcgt cggatcagat tcagtttaag aatacagtcc tgattgattt
     3421 gaatccctca gaagaggaac tacttgcgca catgaaacaa aagactcgct acaacatccg
     3481 tctggcgcag aagaaaggcg tctcggtgcg cgtaggcatg tcagcggatt tggcgctgct
     3541 ttacaggatg tatgcagaga catccatacg cgatggattt gtgattcgcg atgaagggta
     3601 ttacaaaaca gtttggaatc tgtttatgaa ttccgctgac ccatactgtg aaccattgat
     3661 cgctgaagtg gatcatcaac ctgtggctgg aatctttgtg ttttatttcg caggtcgcgc
     3721 ttattacgtc tatggaatgt cgcgggatgt ccatcgtgaa aaaatgccgg cctatttgtt
     3781 gcaatgggaa gcgatgaaac gtgcgaaggc aagaggctgt gccgtttatg acttatgggg
     3841 cgctccggat atattcgatg aaagcgattc catgtggggc gtgtatcgat tcaaggaagg
     3901 cttaggtggt aaagtcgtgc gcacgctggg tgcctgggat tacgcgccga atcgactctg
     3961 gtacaacatg tattcagaga tcattccgcg tgtgctggac gtaatgcgtt cgcgcggtaa
     4021 agcaaagacg aaacagaact tgggcgcgta atagctccga tggttgagta gggcggatgc
     4081 tctttcgccc gtatcgaaac catcatgtta tagaaacact tttgtttgca cggtggtctc
     4141 gatacgggct tcaccctact cgaccaccaa ggaagactaa aattaggaat tctgatgaat
     4201 agcattcccc gtctgcattt tgcacatctt cccacaccga ttgaagaact cccgcgtctt
     4261 tctgagatgt taggaggacc gcgcattttc gtcaaacgcg atgatcaaac cgggttagct
     4321 tttggcggca acaagactcg taaacttgaa ttccttttag cagaagcact tgagcaaggc
     4381 gcgaagacgt tgatcacatg tggcgcacta cagtccaatc actgccgtca gacggcggct
     4441 gccgcggctc gattcggttt tgaatgtatt cttgtgatga acggtgaaat gcctgaacag
     4501 ctatctgcaa atttactgct ggatcggcta ttcggtgctg aaatcgtatt agtaaaagac
     4561 agagcagcgc gcgagcaagc tttgcagaat acatttgatc aggctctcgc cgcgggaaag
     4621 aaaccctatc tcatcaccct cggaggatcc aactcaattg gcgcgttagg gtatgcattc
     4681 gcgatgaagg aatttatgga gcaaaatgtg gatgctgact ggatcctatt tggcacatcc
     4741 tcaggcggta ctcatgcagg acttgtgctg ggtcagcgtg tgtttgactt caaaggaaaa
     4801 gtgttaggga ttagtattga cgaaccagaa gcatggttga agaggcgcgt ctctgaactg
     4861 gcatccgatg caagtgaaaa actcggggag cgaattgaat ttacacgcga ggatgtcctg
     4921 gccaacgaag agtattgtca ggccggctac ggtgtcttga ccgatgcaga acgcgaagct
     4981 gtaaaattat ttgcgaagta tgaaggtcta ttgctcgacc cggtttatac cggccgggcc
     5041 gcagcaggga tgattgattt gatgcgcaag ggatttttca agagggatga aacgatctta
     5101 ttctggcaca ccggcggggg acccgcattg tttgcagaaa aatacgcaaa cctgatatga
     5161 aaaaacggat gagttcaact catccgtttt gtttgaacta cgactttaga gcaccccagc
     5221 ctgcatactt ggcagtctca ccgagttcag cctctatcct aagcaactga ttatatttcg
     5281 caacccgatc tgaccgcgca ggtgcacctg ttttgatctg cccggaatta agtgcaacag
     5341 cgatatcggc gatggttgta tcttccgtct cgccggagcg atgactggtg acggctcgcc
     5401 atcccgcccg ctggcaggtt tccacggctt caattgtctc agtgagggaa ccaatttgat
     5461 ttaccttcac caggagggca ttcgccgcgt tttctttgat cgcccggcga acccgttcgg
     5521 cgttagtgac cagaagatca tcaccgacca gctggcattt attgccaacc ttttgaacca
     5581 tcaac
//
LOCUS       DASTJP010000766         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_596055, whole genome shotgun sequence.
ACCESSION   DASTJP010000766 DASTJP010000000
VERSION     DASTJP010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596055"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFQ23_22345"
     CDS             <1..221
                     /locus_tag="VFQ23_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909405.1"
                     /translation="EWSLDFALPLTFIAMAVPILKNRPTIAAAVSAGIVALVSYSLPY
                     RLGLLVAALVGITVGTLLESRKSSKAAE"
     gene            218..532
                     /locus_tag="VFQ23_22350"
     CDS             218..532
                     /locus_tag="VFQ23_22350"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET9909406.1"
                     /translation="MNIWLVMIFGGLITFAIRFVLIYLFGTFEIPETMRKALHYVPPA
                     VLSGFIFPELLLRDGVINVSLDNHRLFAGLLAILVAWFSRNTLVTILTGMAALFLFQF
                     FF"
     gene            complement(621..1532)
                     /locus_tag="VFQ23_22355"
     CDS             complement(621..1532)
                     /locus_tag="VFQ23_22355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9909407.1"
                     /translation="MKILVLNNDLMERTVIQQVLQRNGHEIIPAENSDIGMHLLQQGD
                     IRFVIADRATTDIDEKQFIKRVREARPPYYIYILLIAMRVQDTDVTAPRMGADDYLHK
                     PIVPIELKSRVYIGERILALGDNLSEAKGDAKNTAVFDPLTNTLNQSAFLTLSRGELE
                     RARRAQSPLSLIALDIDNFKAINDKHGMDIGNDVLTLVAQAIREKSRPYDGVARYEDD
                     LFLLILPGVIGQDAEKIAERIIKGIYNINVSLMDGTAVNVKLSAGIASAMQITASTEI
                     ENLIQKSTEAVRQAKREGGNQVYIVFL"
     gene            1637..1708
                     /locus_tag="VFQ23_22360"
     tRNA            1637..1708
                     /locus_tag="VFQ23_22360"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1669..1671,aa:Met,seq:cat)
     gene            1754..>2407
                     /locus_tag="VFQ23_22365"
     CDS             1754..>2407
                     /locus_tag="VFQ23_22365"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9909408.1"
                     /translation="MDTQIIKNHRINILGDDNLEVWIQSFLSDRKSQNLAKGTLYFYQ
                     GKLKTFLKYADSQAITNVSQISPPFIRDFILFLEQSNHNEGGVSTYYRSLKTFLRWYW
                     EEVEPEFTNPISKVKTPRVSIEPLQGITKEKFDILVAACPRNDFLGERDRTILNVLYD
                     TGIRAEELCNIKITDVSLAENWILISHGKGRKMRLVFFGKTTRKQIRRYMKLRITNDS
                     "
BASE COUNT          607 a          551 c          587 g          662 t
ORIGIN      
        1 aagaatggtc actggatttt gccctgcctc tcacctttat tgccatggct gttcccatat
       61 tgaagaatcg accaacgatt gccgcggctg tctccgcagg cattgtggcc ctggtctcgt
      121 attcattacc ctaccgtctt ggcttgctcg tggcggcgtt agtaggaatc acagttggaa
      181 ccttgctgga gagtcgaaaa tcatcaaagg cggctgaatg aacatctggc tggtaatgat
      241 atttggcgga ctgatcacct tcgctatacg gttcgtgtta atttacttat tcggcacgtt
      301 tgagatcccg gagacgatgc gtaaggcgct gcactacgtc ccaccggctg tgctttccgg
      361 gttcatcttt ccggagctgt tgctccgaga tggcgtaatt aatgtatcgc tcgataacca
      421 tcgtttgttc gcaggtctgc tggcgatcct tgtagcgtgg tttagcagga atactttagt
      481 taccatcctg accgggatgg ctgctttatt cttgtttcaa ttcttttttt aatcaggaaa
      541 aggtagcaac gaacatgttc gtctaacgta aaagtgactg acccacaggg gcacttcgta
      601 gagtcgctac tacagatttt ttagagaaag acgatataga cctgatttcc accctcacgc
      661 ttggcttgcc tgaccgcttc agtggatttc tggatcaggt tctcgatttc ggtggaagcc
      721 gtgatctgca tggcagaggc aatgcccgcg ctcagtttga cattgaccgc ggtgccgtcc
      781 atcagggaaa cgttgatgtt gtaaatgcct tttatgatcc gttcagcgat cttttccgca
      841 tcctgcccga tcactcccgg taggatcaat agaaacagat catcttcata acgtgccaca
      901 ccgtcatacg gacggctttt ctcacggatc gcctgggcga ccagagtcag tacgtcattg
      961 ccgatgtcca tcccgtgttt gtcattgatg gctttgaagt tatcgatatc cagtgcaatg
     1021 agactcagag gggattgagc ccggcgggca cgttccagct ccccgcgtga caaggtcagg
     1081 aaagccgatt ggtttaacgt attcgtgaga gggtcgaata cggccgtgtt ttttgcatcg
     1141 cctttggcct cggacaggtt atcgcccaat gccagaatgc gctccccgat gtagacgcgc
     1201 gacttcaatt caatcggcac gatcggttta tgcaggtaat catctgcacc catgcgcggc
     1261 gcagtgacgt cggtatcttg cactcgcatg gcgatcagta gaatgtaaat gtaataggga
     1321 ggccgagctt ctcgaactcg cttaatgaat tgtttttcgt cgatgtcggt ggtggcgcgg
     1381 tctgcaatca caaatcggat gtcgccctgc tgaaggagat gcataccgat atcactattt
     1441 tcagcaggga tgatctcatg accattacgc tgcaacacct gctgaatcac ggtgcgttcc
     1501 attaagtcat tgttcaggac gagaattttc ataaatgggc gcgcctcctg acttgattat
     1561 atctcagacg ctcttatttc tgcctgtgtt gacaaaccta tcctcccata gtaatatttg
     1621 cggcgctttc ctaatcgccg aggtagctca gtggtagagc aggggactca taagcccttg
     1681 gtcgggagtc caaatctccc cctcggcact gaactctata aatcctaagt ttttgatggt
     1741 ctgaagagga aatatggaca ctcagatcat caaaaaccat aggattaata ttctggggga
     1801 tgataattta gaggtatgga tacagtcttt tctcagcgat agaaaatccc aaaatcttgc
     1861 taaaggaaca ttgtattttt atcagggaaa gttaaagact ttcctgaagt atgccgattc
     1921 acaggctatt acaaatgtct cacaaatcag cccacccttc ataagggact tcatactatt
     1981 tttggaacag tctaaccaca atgaaggagg tgttagcacc tactataggt ctttgaagac
     2041 gtttctacgt tggtactggg aagaagtaga acctgaattc actaacccaa ttagtaaggt
     2101 aaaaactcct agagtttcaa ttgaacccct acaaggaatc accaaagaga agtttgatat
     2161 tctggtcgcc gcatgtccaa ggaatgactt tctaggtgaa agagatagaa ccattttgaa
     2221 tgtgctatac gatacaggga tcagggcaga ggaactttgc aacatcaaaa taaccgatgt
     2281 gagtctggca gagaactgga tactaatttc tcatgggaag gggaggaaaa tgaggcttgt
     2341 gtttttcggc aagaccacga ggaagcaaat caggagatat atgaagttac gcattaccaa
     2401 cgatagt
//
LOCUS       DASTJP010000767        11775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_69109, whole genome shotgun sequence.
ACCESSION   DASTJP010000767 DASTJP010000000
VERSION     DASTJP010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69109"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VFQ23_22370"
     CDS             complement(<1..1277)
                     /locus_tag="VFQ23_22370"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9909409.1"
                     /translation="MMDDSIHILLIEDDPAHAELIQRAFEDRGDGAHLTIAHNLEEAS
                     IQIKKLKPTLIIADWRLPDGDSTTLLQEHSHATAPPIIIMTSYGSERNAVDAIKAGAL
                     DYIVKSSESMTDMPHIAERAVQQWKSVQEQKRMQDALAERESQFRLLAENSSDIISRH
                     DAKGIFLYVSPACRNILGYEPEELIGTSFLSYIHPDETKQLEELLSSSSWNDPTLGIP
                     YRASRKNGEYIWLETTARVIFDDENRMVEIQASSRDITERKEAQEALQQAHEGLREAY
                     DKTIEGWVVALDLRDRETEGHTQRVTQMTVRLAREFRFTEEEIVHIRRGALLHDMGKM
                     GIPDEILQKPGPLTDDEWMIMRRHPQYAYQMLSPISYLNQALIIPYYHHERWDGSGYP
                     QGLKGDQIPLYARLFAVVDVWDALSSDRPYRKR"
     gene            complement(1310..1759)
                     /locus_tag="VFQ23_22375"
     CDS             complement(1310..1759)
                     /locus_tag="VFQ23_22375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909410.1"
                     /translation="MVGEPILVMLVEDNVDHAELVIRTMEEHRITNKVRHFLDGQSAL
                     DYLFYRGEFSDPLNSPRPHVILLDLRLPRVDGIDVLKAIKEDENLKTIPVVVLTTSEA
                     EKDVARAYYNHANSYLVKPVGFEEFKKLMDDLGFYWLGWNTNPENRE"
     gene            complement(1860..4076)
                     /locus_tag="VFQ23_22380"
     CDS             complement(1860..4076)
                     /locus_tag="VFQ23_22380"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909411.1"
                     /translation="MITRPAMKFLFSLPEFGNEELNIKGRLVYLVASSVVILTLIFVL
                     AIPFVAPSLTRRSLLLAISILPTCIGIMALVHRSKLRLASNLLLLTLWLVVTGGSITA
                     GGISAPIFVGYFIVIIISGLISRRRIRFYVLLLCILTGIILVYAELTGSLPPPTSYSP
                     QARIFIYSFFFSIALLLQNITSGNIGRLLKRAKEIETRYKSLLENIPSITYINSLDVE
                     SFTQYVSPQVEHLAGYSQKEFMEDNLLWTKILHPDDREQVLAENARTVESGERFQMEY
                     RVLTKDQQTIWVHDEANLLYDSEGMPLYWLGVWTDITSRKLAEEEQANLIGVLTKHTL
                     QLQTAAEVSNAVSSVLDIDELLINVVELIRHHFDYYYTGIFMVDETREWAVLKAATGE
                     MGRQMLAQGHRLKIGNSSMIGWSIFHRKARIALDVGEDAVRFKNPNLPLTRSEIALPL
                     IAHGEVIGAMTIQSQSPSAFSRMDVTVLQTMADQIANAIENARLFTERASLINELGGQ
                     NAELERFTYTVSHDLRSPLVTIRGFLGYLRQDAASGDLSRFDTDIARISNAVDKMQVL
                     LNDLLELSRIGRIINPPEEIPFEHIVKESIEMTSGLLEARNVRVEIQKELPIIYGDRT
                     RLLEVMQNLISNAIKFMGDQAVPRIEIGTTESQLDGKPVFFVRDNGIGIEAQYHERIF
                     GLFNRLDPSVDGTGIGLSLVRRIIEVHNGKIWVESEPGIGTTFLFTLPLAQLVAKD"
     gene            4206..4541
                     /locus_tag="VFQ23_22385"
     CDS             4206..4541
                     /locus_tag="VFQ23_22385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9909412.1"
                     /translation="MEITVQEFKHCDMITIKGRIDSATAPQLAQALEAANNNGKYKLV
                     VNMSELEYMSSAGFRALLAAQRNSKRYNRGEVVLAAVPDRIREALELAGFTELFKTFD
                     DPLLAVGHF"
     gene            4639..5052
                     /locus_tag="VFQ23_22390"
     CDS             4639..5052
                     /locus_tag="VFQ23_22390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909413.1"
                     /translation="MQSVQFPAKFDYLDEIRDFVGEIARTGGFGSKEVYNIQLATDEA
                     ASNIIEHAYEGISDGVLELSCGFQGGAIVIILVDHGEPFDPSEIPMPDLKADLSERKI
                     GGLGIFLMRKLMDEVHYDSQPGKNSNTLTMIKRKR"
     gene            5059..8346
                     /locus_tag="VFQ23_22395"
     CDS             5059..8346
                     /locus_tag="VFQ23_22395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9909414.1"
                     /translation="MSAASRLPHSSDWRDLASLGEQILSAPSLAAQRDRIIAMASRLV
                     DGNVEVWLHENMFRLPDTEDELLFPEQPSTPGMQRALRLGKMSTKQKREAKNGASRET
                     WAAIPLEDQGIVLGALQVTRAKGPEFRQEELDVLEGLAGVIIVSLIASHRVAVEKFRL
                     NQLHLVSAVSAQIANVLNLDELTRRVTKLIQQTFHYYYVAIFTLRDRSTAMRYRASAM
                     SKATRLEKTSRKRKPVVKPKVAVEVEIGQGLIGQAAETGERIVIGDVRKDTRYRFIDA
                     LPDTRSEAVIPLKIEDRVLGVLDVQSDQLNGFHPNDLLILGALADSIARAVEGTRLYN
                     NLRRRADQLTLISEVSKSVSSSLELGKLMTDVAHLIHDRFRFPYVQLLTVHPNRGLIE
                     YQAGSGERSEGLAGYTLSLEDEQGIIPWVAREGKVVLANNVKKDKRYRASPLPPDDTK
                     SELSVPLLYDNEVVGVLDIQSNKYNAFTDEDQRMFEAVADNIATAIHNADLYRTEQWR
                     RQVGDGLREVAGLISAEADLDDVLDAILSELDRNLSVDISAIWLLDDEELYLAASHNV
                     DETLLENTLYDSPESYDALMRVVQSDGPQVRKPTDPVWVSGLAAGFDQHYSALAVPLR
                     LGDQPLGVITLAHQAPGKYGHESQAMTTTFASYAAVAIENARLYDAAQEQAYASAALL
                     QVAQAIVSLSDLDEILGTIIRIMPILVGVQRAALYQWDAEKEIFRPSQEYGLDDEDEN
                     RFWERSFAPGEFAFLDACRDSTGLLACQLDEQSGLNAWLSVYPDHDVNLGSSGTLLFA
                     VPIAVKDDLYGVMMIEEAVGGLRFRARRLEIITGIAQQAALAIQNDLLQKEMFVRERL
                     ETEVQLARQIQQTFIPESLPQFSNWELAARWKTARQVGGDFYDVFDLPNNRLGLFIAD
                     VADKGVPAALFMALTRTLVRAAVIETDSPAEAMRRVNDLLVPDTRQGMFVTAVYAVLD
                     LDSGELTYVNAGHNPPLWVKQDGSIERLTRTAIALGVVTGDSVDQRTIKLETGDNILL
                     YTDGLTESFNAADEFFGEKRLLDAIVSSGCDSASNLLDVVEAALLNFVQDMPPADDLT
                     MLVLRRT"
     gene            8460..8870
                     /locus_tag="VFQ23_22400"
     CDS             8460..8870
                     /locus_tag="VFQ23_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9909415.1"
                     /translation="MYTTEGKKIVYTDKAPKTLGPYSQAIRTENLVFAAGQLGVDPAT
                     MELVEGGIDAQTRQALTNLKNVLEVADSGLNFIVKTTVFLQDMRDFPNMNAVYAEYFP
                     ENPPARSTVQVAALPRNALVEIECIALLSSTRGD"
     gene            8942..9859
                     /locus_tag="VFQ23_22405"
     CDS             8942..9859
                     /locus_tag="VFQ23_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909416.1"
                     /translation="MNNGSLTKFWPLIYSTVRELWQITEPQIEDAAIRYDIPVELYVY
                     SELGLEYFSIADFQKRDPFSNTEQFEKLYARLLVKGWIAALPDGRYQVTEKARGATRH
                     IIQAGDTHLATFEPLLEIDLKKLAGLLQRLFIASAEAAEPPQKWGILNRFRVADENSP
                     WLAQIREYMMDLFAYRDDSHLAASHPHFGGAGIVWSVLGSIWKGDAVTPEKMAESMAF
                     RGYEVDDYEVAIQAAIELGWVESADVPFSFRLTPQGRELREQAEQLTNEYFYRPWSVL
                     DQDDLADLYDLLLKLRDQLRELSRNKTSL"
     gene            10077..11081
                     /locus_tag="VFQ23_22410"
     CDS             10077..11081
                     /locus_tag="VFQ23_22410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9909417.1"
                     /translation="MQPKVSIIVPCYNEQSTIRLLLEALREQTFPRADMEVVIADGIS
                     QDGTRDAIALFQRDYSDLDVRVVDNKSRTIPAAVNRAIQASRGEIIVRMDAHSKPYPD
                     YVQNCVNALEGNLGDTIGGIWEIHPGAESWVAKSIAVAAAHPLGVGDAFYRHTKQAAE
                     VDTVPFGSFRRSLIDRVGYFDESLLTNEDYEFYVRVKKAGGRIWLDPSIRSIYFARAT
                     LSQLARQYRRYGFWKWRMLRRYPHTLRWRQALPPLFVLSVIVLGLVSLFIPFVGILLA
                     IEILLYFSILLFVGLRVALRRRKHYLLFGLPLAISIMHVSWGGGFLWSILTAGFQKNG
                     "
     gene            11074..>11775
                     /locus_tag="VFQ23_22415"
     CDS             11074..>11775
                     /locus_tag="VFQ23_22415"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909418.1"
                     /translation="MAENVRTTPLRLRPSEHRTILFVGDLVTAIVSVFTALYTWRQYN
                     FSQRDGQIAELIERGFSQARAVSIVEQRSIEVPFWFYLLPLLWMLLLVELYEPHVAGS
                     GRKTTRGIAIAAFVGLAVYSLVFTLRQDPTNLPRIGVGAFLFFASILTLVWRVLFIRI
                     YKTTGQRRRILLIGAGKAGQTLAELYRNEGMRSFNIVGFIDDDMMKVGQNFHGLPVIG
                     TSKQLLNLIDVYRISD"
BASE COUNT         2861 a         2812 c         3052 g         3050 t
ORIGIN      
        1 atccgcttac gatatggccg gtcactggag agcgcatccc agacgtcgac caccgcaaag
       61 aggcgcgcgt acagtgggat ttgatcgccc ttcagcccct gcggatatcc actgccatcc
      121 caccgttcgt gatgataata ggggatgatc aaagcctggt ttagataact aatgggcgag
      181 agcatttgat aagcatattg tggatgacga cgcatgatca tccattcatc atccgttaac
      241 ggccctggct tttgcaggat ttcatcggga atgcccatct tacccatgtc gtgcagtaac
      301 gcaccacgac gaatgtgaac gatttcttct tcggtgaacc gaaattcgcg cgccagccgt
      361 acggtcattt gtgtgactcg ttgcgtatgt ccttcagttt cgcggtcacg caaatccagc
      421 gcaacgaccc acccctcaat cgttttatcg tacgcttctc gcaagccctc atgggcctgc
      481 tgtaatgctt cctgagcttc tttccgttcg gtaatatcgc gggaggaagc ttgaatttcc
      541 accatcctat tttcatcatc aaagattacg cgcgcagttg tttccagcca gatatattca
      601 ccattcttgc ggctggcgcg gtagggtatt cccagagtcg ggtcattcca tgaagaagat
      661 gacaaaagct cttccagctg tttggtttca tctgggtgaa tataagacag gaatgaagtg
      721 ccgatcagtt cctcaggttc gtagcccaat atgttgcggc atgcaggtga aacgtagaga
      781 aatattcctt tggcatcgtg tcttgaaatg atgtcagatg agttttctgc cagcagccgg
      841 aattgcgact ctcgctcggc aagcgcatcc tgcatccgtt tctgttcctg cacgcttttc
      901 cactgttgaa cggcacgctc cgcgatatgt ggcatatccg tcatcgactc ggacgacttg
      961 actatatagt ctaatgcccc agcttttatg gcatccaccg cgttacgttc actgccataa
     1021 ctggtcatga taatgatcgg tggggcagtg gcatggctgt gctcttgaag aagtgttgta
     1081 ctatcgccat cgggaagtcg ccagtctgca atgatcagcg tgggcttaag tttcttgatc
     1141 tgaatgctgg cttcttcaag attgtgcgcg atggttaaat gcgctccgtc tccgcggtct
     1201 tcgaaggcgc gttgaatcaa ttctgcatgg gcaggatcat cttcaatcaa cagaatatgg
     1261 atggaatcat ccatcatatg gatctatcca gagctttaac gatcttattt tattccctat
     1321 tctccggatt ggtgttccag ccaagccaat aaaagcctag atcatccatg agcttcttaa
     1381 attcttcaaa tcccactggt ttaacaaggt aactgtttgc gtgattgtaa taggctctgg
     1441 ctacatcttt ttcagcttca gaggtagtga gcacgacaac tgggatggtt tttagatttt
     1501 cgtcttcctt gatcgccttt agtacgtcga tgccatccac gcgcgggagt cgaaggtcca
     1561 gcaggatcac atgcgggcgt ggactgttaa gtgggtctga aaattcccct cggtagaata
     1621 gataatctaa tgctgattgg ccgtccagga aatgacggac tttgtttgtg atgcggtgtt
     1681 cttccatggt gcggatcaca agctctgcat gatccacatt gtcctctacc agcatgacca
     1741 ggattggctc accaaccatt tgattctcct acggagcgca gcgcatattt caaatatatg
     1801 cagaagctgc aactgcctgc gtgattttaa tcccaattcc tattttggga tagttggtct
     1861 cagtctttag ccacaagttg ggctaaggga agggtaaaca ggaaggtagt tccaataccg
     1921 ggttcagatt ccacccatat ctttccgttg tgtacctcaa taattcgccg gacaaggctt
     1981 agtccaattc cagtgccgtc aacacttgga tcaagccgat tgaatagtcc gaaaattctt
     2041 tcgtggtatt gagcttctat gccaatgcca ttatctcgta cgaaaaatac tggttttcca
     2101 tccagctgcg actcggttgt gccgatctca atgcgcggta cagcctggtc acccatgaat
     2161 ttgatggcat tactgatgag attttgcatc acttccagca aacgagtacg atctccgtag
     2221 ataatgggca attccttttg aatttcaacc cggacgttac gagcctcgag cagcccgctt
     2281 gtcatttcta tgctctcctt cacaatgtgt tcaaacggga tttcctccgg tggattgatg
     2341 attcgaccga tgcgggaaag ttccaataaa tcatttagta aaacctgcat tttgtcaacg
     2401 gcgttcgaaa ttctggcgat atccgtatcg aagcgagaga ggtctccgga ggcagcatcc
     2461 tgccgaagat accccaggaa gccgcgtatc gttacaagcg gcgaacgcag gtcatgagag
     2521 actgtatacg taaagcgttc aagctccgca ttttggcctc caagttcatt gattaaggag
     2581 gcgcgttctg tgaacaagcg agcgttttca atggcgttgg cgatctggtc tgccatggtt
     2641 tgaaggacag tgacatccat ccgcgagaag gcagaagggg attgagattg tatcgtcatg
     2701 gcaccgatta cctctccatg cgcaatcaaa gggagcgcga tttccgaacg cgtcagagga
     2761 agatttggat tcttgaatcg aacggcgtcc tccccgacat ccagcgcgat gcgtgccttc
     2821 cgatggaaaa tagaccagcc aatcatcgag gagttgccaa tcttaaggcg atgaccctgg
     2881 gcaagcatct gtcttcccat ttcaccggtg gctgctttga gaactgccca ttccctggtt
     2941 tcatcgacca tgaaaattcc cgtgtagtaa taatcaaaat gatgtcgaat gagctccaca
     3001 acattgatga gaagctcatc aatatccagc acagaagaca ctgcatttga aacctcagca
     3061 gccgtttgaa gttgtaatgt gtgtttggta agaacgccga tcaaattcgc ttgctcttct
     3121 tcggccaatt tgcgagaggt gatatctgtc cacacgccca gccaatacaa aggcatgcct
     3181 tctgaatcat ataagaggtt tgcttcgtcg tgtacccata tggtttgctg atcctttgta
     3241 aggacgcgat attccatttg gaagcgctcg cctgactcta cggtccgcgc gttttcagcc
     3301 aatacttgct cgcgatcatc gggatgcagg atctttgtcc acagcaaatt atcctccatg
     3361 aattcctttt gagaatatcc tgccaggtgt tccacctgag ggctgacata ctgtgtaaat
     3421 gattcaacat caaggctatt gatgtaggta atgcttggga tgttttccag aagcgattta
     3481 tatcttgttt cgatttcctt tgcccgtttc agtaatcggc cgatattccc cgaggtgata
     3541 ttctgaagga gcagcgctat ggagaaaaaa aacgagtaga tgaaaatacg tgcttgtggt
     3601 gaatagcttg tgggcggtgg aaggctgccg gtcaattcag cataaacgag gattattccg
     3661 gtcaatatgc aaaggagcag aacataaaac ctgattcgtc ttctggatat taacccgctg
     3721 ataatgatga cgataaagta tccaacgaag atgggcgctg aaatcccgcc ggcggtaatg
     3781 cttcctcctg tcacaaccag ccagagcgtg aggagcaaaa ggttgctggc tagtctcaat
     3841 tttgagcgat ggaccagcgc cataatccca atacaggtcg ggaggatcga aattgctaag
     3901 agtagtgagc gtcttgttaa gctgggtgct acaaacggaa tggcaaggac aaagatcagc
     3961 gtaagtatga cgacagaact tgcgaccaga taaaccaagc gccctttgat atttagttct
     4021 tcgtttccga actcaggtag tgaaaaaaga aacttcatgg ccggtctggt aatcaagtgc
     4081 caagtatagc atcccttaac gagttcacat ttgcacaact ctcgcaatgc cctggtggga
     4141 gtggatttat tatcatgata taattttgca gttcaacacc gtactttcga tttggaggat
     4201 gttctatgga gatcacggtt caagagttca aacattgcga tatgatcacg atcaagggtc
     4261 gcatagatag tgcgactgcc cctcaacttg ctcaggcact tgaagccgcg aacaataacg
     4321 gcaaatataa gttggttgtg aacatgagcg aattggaata catgtccagc gcgggttttc
     4381 gagctctgct ggcagcacaa cgcaatagca aacgatacaa ccgtggtgaa gttgtattgg
     4441 cggctgttcc agatcgaatc cgcgaggcgc ttgaactggc tgggtttacg gaactcttca
     4501 aaacctttga tgatcctttg ctcgccgttg gccatttcta agggctgtgt acgcagaaaa
     4561 acctggagaa cgcatacaat acttgaactg atgccgccct cagcgatcca tcactgatgg
     4621 cggctcttaa tttatcgaat gcaatctgtt caattccctg cgaaatttga ttacctcgac
     4681 gagatccgtg attttgttgg cgagattgcg cgtaccggcg gcttcggcag caaagaggta
     4741 tataacattc aacttgccac ggacgaagcg gcttccaaca ttatcgagca tgcgtatgaa
     4801 ggtatcagcg atggggtcct tgaactttcc tgcggatttc aaggcggcgc gattgtcatt
     4861 attctggtgg accatggaga gccgtttgat ccctccgaaa tccccatgcc agacttgaaa
     4921 gccgaccttt cggaacgcaa aatcggggga ctgggaatat tcctgatgcg aaaactcatg
     4981 gatgaggtcc actatgattc gcaaccgggt aaaaacagca acaccctcac aatgatcaaa
     5041 cgcaagagat aggacggcat gtcagccgct tctcgcttgc ctcattcttc ggattggcgc
     5101 gaccttgcca gcctggggga acaaatcctc agcgcaccgt cgctggctgc gcaacgtgac
     5161 cgcatcattg ccatggcttc gcgcctggtg gatgggaatg tagaggtctg gcttcacgag
     5221 aacatgttca ggctgccaga tacggaagat gaacttcttt tccccgaaca accttccaca
     5281 cctgggatgc aacgcgcgtt aagactgggg aaaatgagca caaaacaaaa acgggaggca
     5341 aaaaacggcg cctcacgcga aacgtgggca gccatcccat tggaagatca ggggattgtg
     5401 ttgggggcct tgcaggtcac tcgagccaag ggaccagaat ttcgacagga agagctcgac
     5461 gtgcttgaag ggctggccgg agttatcatc gtgagcctga ttgcctcgca tcgcgttgca
     5521 gtggaaaagt tcaggctcaa ccaactgcat cttgtaagtg ctgtgagcgc acaaattgca
     5581 aatgtgctca atttggatga gcttaccagg cgggttacaa aactgattca acaaacattc
     5641 cactactact atgtggccat atttacactt cgtgatcgtt ctaccgccat gcggtatcgc
     5701 gcgagtgcta tgtctaaggc aacgcgcctg gaaaagacat cacgcaaacg caagcctgtg
     5761 gtgaagccca aggtggcggt ggaggtggaa attggccagg ggttgatcgg gcaagctgca
     5821 gagaccggag aacggattgt gatcggtgat gtgcgcaaag atacgcgtta tcgattcatt
     5881 gatgccctcc cagatacacg ttcggaggca gtcattccgc tgaagattga agaccgtgta
     5941 ctgggtgtgc tggatgtaca gagcgaccaa ctgaatgggt ttcatccaaa tgacttgttg
     6001 atcttgggtg ctctggccga tagcattgca agagcagtgg aaggcacacg cctttacaat
     6061 aatttgcggc gtcgtgccga tcagttgacc ttaatatcag aagtcagcaa gagcgttagt
     6121 tcatcgcttg aattagggaa gttaatgaca gatgttgcac atttgatcca tgaccgattc
     6181 cgttttcctt atgtgcagct gctcacggtc cacccgaacc gtggattaat tgaatatcag
     6241 gccggtagtg gtgaacgtag tgaaggcttg gccggctaca ctctttcact ggaggatgaa
     6301 cagggaatta ttccctgggt ggcgcgtgag gggaaggtgg tcctggcgaa taatgtgaaa
     6361 aaggataagc gttaccgtgc ctcgccgctg ccccccgatg atacaaaatc agaactctct
     6421 gttccacttc tgtatgacaa tgaagttgta ggggtgctcg atattcaatc gaacaaatac
     6481 aacgcgttta cagatgagga tcaaagaatg tttgaggcgg tcgcagataa catcgcgaca
     6541 gccatccaca atgccgactt gtaccgaacc gagcagtggc ggcgacaggt cggagatggt
     6601 ttgcgggaag ttgcaggttt gatttctgca gaagcagatt tggatgatgt gctggatgcc
     6661 attctatctg aactggatcg caatctctcg gtcgatattt cggcaatctg gctgctcgat
     6721 gatgaagagt tgtatctggc agccagccat aacgtcgatg aaacactttt agaaaacacg
     6781 ctgtatgatt cacccgaatc ctatgatgct ctgatgagag tcgttcaatc agatggtcca
     6841 caggttcgca agccgactga tccggtttgg gtttcgggtc ttgcggctgg cttcgatcaa
     6901 cattattcgg ctctggctgt cccattgcgt ctgggagacc agcccctggg cgtgattacc
     6961 ctcgcgcatc aggcaccggg caagtatggg catgagtctc aggcaatgac cactacattt
     7021 gcaagttatg ccgcggttgc gatcgagaac gcgcggcttt acgatgcagc acaggaacag
     7081 gcctatgcct ctgcagcctt attgcaagtt gcacaggcga ttgtcagctt gagcgacctg
     7141 gatgaaattc tggggacgat cattcgcatc atgccaattc ttgtaggtgt gcaacgggcc
     7201 gcattgtatc aatgggatgc ggagaaggaa atcttccgac cgtcacagga atatggattg
     7261 gatgatgaag atgaaaatag attctgggag cgttcctttg cacctgggga attcgccttc
     7321 ctcgacgcct gccgcgactc gaccggtttg ctggcatgtc agctggatga gcaaagtgga
     7381 ctgaacgcct ggctttccgt gtatcctgat catgatgtca atcttggctc gtctggaacg
     7441 ttgttgtttg ccgtacccat tgcagtcaag gatgacctct acggcgtcat gatgatcgaa
     7501 gaagccgttg gaggcttgcg cttccgcgcc cgccgactgg agatcatcac gggcattgca
     7561 cagcaggcag cacttgccat tcaaaatgac ctcctccaaa aagagatgtt tgtgcgcgaa
     7621 cgcctcgaaa ccgaagtaca actggcgcgg caaattcagc agactttcat ccccgagtcg
     7681 ttgcctcaat tttcaaattg ggaactcgcc gcgcgctgga agacggcccg tcaggtgggc
     7741 ggtgatttct atgatgtgtt tgacctgccg aataaccgac ttggcctctt catcgcagat
     7801 gtcgcggaca aaggcgttcc ggctgccctc ttcatggcgc tcacacggac gttggtgcgt
     7861 gcggccgtta ttgaaactga ctcgccggcc gaggcgatga gacgcgttaa tgacctgctc
     7921 gttcctgata cccgtcaggg aatgttcgtt acggctgtgt atgcagtatt ggatttggat
     7981 agcggcgagc tcacgtacgt caatgcaggg cacaacccgc cgttatgggt taagcaagat
     8041 gggagtattg aaagattgac tcgcactgcc atcgcgcttg gtgtcgtgac aggcgactct
     8101 gtcgatcaac gaacgatcaa actggaaaca ggagataaca tcctgcttta tacggatggt
     8161 ttgaccgaat ccttcaacgc ggccgatgaa tttttcggcg agaaacgtct tctggatgca
     8221 atcgtttcca gtggttgcga ttctgcctcc aatttgttgg atgtggtcga ggccgcgctg
     8281 ctaaattttg tgcaggatat gccccctgcc gatgacctga cgatgcttgt gctgagaagg
     8341 acatagaacg caaccgggca agggaggatg tacagattga tatgaaaaaa aagtttacac
     8401 aaacctcaaa tcagcagtaa aatttcaatt caccctaaaa tccaaaaacg tgaggaatca
     8461 tgtacacaac tgaaggcaag aaaattgtct acaccgacaa ggcaccaaaa acgcttggtc
     8521 cgtattcgca agcgattcga acggaaaatt tggttttcgc cgcaggtcag ctgggtgttg
     8581 accccgcgac aatggaactc gttgaaggcg gcatcgacgc acaaacccgt caggccctga
     8641 ccaaccttaa gaacgtgctg gaagtggctg actcaggctt gaacttcatc gttaaaacaa
     8701 ctgtcttcct gcaggacatg cgcgactttc ccaacatgaa tgcggtctac gcggaatatt
     8761 tccctgaaaa tcctcctgca cgcagcacag tacaggtggc ggccttgcca cggaacgcgc
     8821 ttgtagaaat cgaatgtatc gcgctgctct cttctacgcg tggtgactga tccccatcca
     8881 atgatattgt aattttcaat catagttttg gtcggataat agacatccac acgttaccca
     8941 tatgaataac ggatcgctga ccaagttctg gccgcttatc tactccacag tcagagaatt
     9001 gtggcagatt accgaaccac agattgagga tgccgcaatt cgttatgata ttccggtcga
     9061 gttatacgtt tacagtgaac tgggactgga atatttttca attgccgatt ttcaaaagag
     9121 ggatccgttt tccaatacgg aacaatttga gaaactatat gcccgccttc tcgtcaaagg
     9181 ttggattgcc gcgctcccag atggtcgtta ccaggttact gagaaagcac gcggtgccac
     9241 acgccatatc atacaggcgg gggatacaca tctcgcaacg tttgaaccgc ttctggagat
     9301 tgacctaaaa aagcttgccg gattactaca gcggttgttt attgccagcg ccgaggcggc
     9361 tgagcctcca caaaaatggg ggatcctcaa ccgcttccgc gttgcggatg aaaacagccc
     9421 ctggcttgcg caaattagag aatacatgat ggacctgttt gcctatcgtg atgattcaca
     9481 ccttgcggct tcgcatccgc attttggagg ggcaggtatc gtctggagtg tgctcggttc
     9541 gatctggaaa ggagatgccg ttacccccga gaagatggcg gagtcgatgg cattccgtgg
     9601 ctacgaggtt gatgactatg aagtcgcaat tcaggccgca attgagcttg gatgggtgga
     9661 aagcgcggat gttcctttct ccttccgcct gacaccccag ggacgggaat tgcgtgagca
     9721 ggcagagcaa ctcactaatg agtattttta tcgtccctgg tcggtattgg atcaggacga
     9781 tcttgccgat ctgtatgacc tgctattgaa actacgcgat cagttacgcg agttatccag
     9841 gaataaaaca agtttgtagg gggttcgcgt ggatttcgca atgggtggtc gtggtataga
     9901 cgactttgcc gcattagcaa aatggcaagt gcatggtggt agaattagcg tccgcagtta
     9961 gttggggcat ggcacaatgc tggtcatcca caaatcgctt gccatgtgaa agaaaatatt
    10021 tttacggatt ctgaattacg cattatcatt gtggttcgta acgcgtaatt tgtactatgc
    10081 agccaaaagt ttcaatcatc gtcccctgtt ataacgagca atccacgatc cgtttattgt
    10141 tagaagcact gcgcgaacaa acctttccgc gtgcggatat ggaagttgtc atcgctgatg
    10201 gtatctcaca ggacggcacg cgcgacgcaa ttgccttatt ccaaagagat tactccgatt
    10261 tggatgtccg tgtcgtggat aacaagagtc gtaccattcc tgcagcggtc aatcgcgcca
    10321 ttcaggcttc acgcggcgag atcatcgtcc gcatggatgc ccattccaag ccctatccag
    10381 attatgtaca gaattgtgtg aacgcactgg aaggcaacct tggcgatacc atcggtggca
    10441 tctgggaaat tcatcccggt gcagagagtt gggttgcaaa atccatcgca gtcgcggcgg
    10501 cgcatcctct gggggtgggg gatgcgtttt atcgtcatac caaacaggct gccgaagtgg
    10561 atacggttcc atttggctcc ttccgtcgct cgcttataga ccgtgtagga tattttgacg
    10621 aatccctgct caccaatgag gactatgagt tctacgttcg tgtgaaaaag gccggtggta
    10681 gaatctggct tgacccttcc atacgctcaa tctattttgc acgtgccacg ttgagccagt
    10741 tggcgcggca gtatcggcgt tatggattct ggaagtggcg tatgctccgt cgctaccctc
    10801 atactctgcg ctggaggcag gctctgcctc cgttgtttgt attaagcgta attgtgttag
    10861 ggttggtctc actcttcatt ccctttgtag gaatcctgct ggcaattgag attttgctct
    10921 atttttccat attattgttt gtgggtttgc gggttgcatt aaggcgccgt aagcattatt
    10981 tgcttttcgg gttgccgctg gcgatttcaa tcatgcatgt atcgtggggc ggggggtttc
    11041 tatggagcat cctcacagcg ggtttccaaa agaatggctg aaaacgttcg caccacgccc
    11101 ctgcggttga ggcccagtga gcatcgcaca attctctttg tcggtgacct ggttaccgcg
    11161 attgtctctg tcttcaccgc tctgtatacg tggagacaat ataatttttc acaacgtgac
    11221 gggcaaatcg cagagttgat tgagcggggc ttttcgcagg cccgtgccgt ttcgattgtt
    11281 gaacaacgca gcattgaagt gccgttctgg ttttatctcc tgccactcct ctggatgcta
    11341 ctgctggtcg aattatacga acctcatgtg gcaggtagtg gacgaaaaac tacccgcggg
    11401 attgcgatcg cagcgtttgt tgggcttgcc gtgtattcac tcgtctttac acttaggcag
    11461 gacccgacaa atctgcctcg tatcggtgta ggcgcgttcc tgttctttgc ctccatcctg
    11521 acgctggtct ggcgcgtgct attcatccgc atctataaga caacaggaca aagacgtcgg
    11581 attctgctca ttggggccgg gaaggcaggg caaactcttg cagaactgta tcgaaacgaa
    11641 gggatgcgtt cctttaatat cgtgggcttt atcgacgatg acatgatgaa ggtgggacaa
    11701 aattttcatg ggttaccggt aatcggtaca tcgaaacagt tattgaatct gatagatgta
    11761 tatcgtattt cagat
//
LOCUS       DASTJP010000768        18097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1125166, whole genome shotgun sequence.
ACCESSION   DASTJP010000768 DASTJP010000000
VERSION     DASTJP010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18097
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125166"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /gene="pgl"
                     /locus_tag="VFQ23_22420"
     CDS             complement(<1..559)
                     /gene="pgl"
                     /locus_tag="VFQ23_22420"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET9909419.1"
                     /translation="MKAELRIFHELERLSRHAANLFVEQAGRSVRERNQFLVALNGGS
                     TPNRLFQLLGTEYRDQVDWGKVQVFWGDERCVPPDEAGSSYGQARELLLRHVSTPESN
                     IHRIKGELPPVEASKDYSSKLKEFAAPPFDWPRFDLVYLGMGEDGHTASLFPGSPVTV
                     SEPTVPVTAHYQDRPANRVTLTQLVF"
     gene            complement(562..1137)
                     /locus_tag="VFQ23_22425"
     CDS             complement(562..1137)
                     /locus_tag="VFQ23_22425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9909420.1"
                     /translation="MQNLFRRILFQYWYFGQPKWDTGISPPELLDFIENHKAARAIDI
                     GCGTGTNVITLAKAGWRVTGVDFAPRAIGLARKKLESNSIEAEVSVSDATQLKGVTGP
                     YELALDIGCFHSIHKDGKANYLKQLNRILAPHGFWLMYGFLKQDPDLAGTGIDPADLD
                     LISAQLTLVSRRDGVDDKRDRKSAWFLFQKP"
     gene            1324..3408
                     /gene="fusA"
                     /locus_tag="VFQ23_22430"
     CDS             1324..3408
                     /gene="fusA"
                     /locus_tag="VFQ23_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9909421.1"
                     /translation="MKEYTTEFIRNIALVSHGGAGKTMLAEAFLHATGATTRLGKVED
                     GTAISDYDEEEHRRKISIYSSVIPLEHRDHKINIIDAPGYTDFQGEAIAALSVADGAI
                     ILVDAVAGVEVGTEIAWRYADEFNLPRFFLINKMDRDNADFEKTYAALEEFVRAHGKR
                     ALKVHLPIGEKQNFKGVVDIIGMKSYLGDGKTTGEIPAELKEEAEAAHFTLVEAAAEG
                     EDELMEKYLENGSLSDEEMVRGLEDVVYAGTFVPIFCAAGAREVGSIALLNDIIDLMP
                     SPLHGPARTAKGKDGSEEKLSASDSAPLAAYVWKTTADPFVGKMTYFRVFSGSIQADA
                     RVWNQNKNAEERMSGLHFQRGKEHIPAKVVHAGDIGCVSKLTVSSTGDTFCDKGHPLT
                     IEKPNFPAALYRVAVTPKTQADAAKISPTLTRLCEEDMTLSWENDKITHETVLKGMGD
                     QHIDVAIHRAQTKFQVGLATHEPKIPYREGITRKANAQYRHKKQSGGAGQFGEVHLRI
                     EPYHDGDFDFGDELVGMNLSKSYLPPIEKGIKATMERGAFAGYPMSNVKVIVYDGKEH
                     EVDSKPVAFEIAGREAFKLAVQDAGPVLFEPIMNVRVTVPDASMGDVMSDLNTRRGRV
                     QGTESERGSTVIMAHVPMSEMTRYTTQLRSITGGRGYFTMDFDHYDVVPTHIAGTIIE
                     AHKREMEAKKEE"
     gene            3778..4728
                     /locus_tag="VFQ23_22435"
     CDS             3778..4728
                     /locus_tag="VFQ23_22435"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET9909422.1"
                     /translation="MTLYDTSLSQTNTDSPTDITSRVGNTPLLSLQRIGRGLSPRVKV
                     FAKAEWFNPGGSVKDRPALNIIQTALANGELGNGKRLLDSTSGNMGISYATFGAALGI
                     PVTLTIPASASPERFTMLRALGAELITTDPTEGSDGAILVARQLASERPDLYWYARQY
                     DNPANWKAHFESTGPEILSQTNGEVTHFVAGLGTSGTLTGTGRYLREQLPDVAILAFQ
                     PDAAFHGLEGLKHMPTAIKPGIYDPFFADEVLEVRTEDAHEMVRRLAREEGLFVGISS
                     GAAAVTALRVANELDEGTVVTVFPDAGYKYLSDKALWEGK"
     gene            4725..5153
                     /locus_tag="VFQ23_22440"
     CDS             4725..5153
                     /locus_tag="VFQ23_22440"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET9909423.1"
                     /translation="MTLNLTDELLARIHAHGEESYPEEGAGFLLGDDSGNSQRAVTEI
                     FPLINSRENEARHNRYLITPEDYLKAELAADKLGLSLIGVFHSHPDHPNQPSDYDREW
                     AQPFFSYIITSVNGGKAVESRSWRLTEDRSKFEEEELKIS"
     gene            5223..5717
                     /locus_tag="VFQ23_22445"
     CDS             5223..5717
                     /locus_tag="VFQ23_22445"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HET9909424.1"
                     /translation="MTSQTLQKVDHSALKTNQIIIISLNILAFILNLPILAAVVALVM
                     GIGSALKVPGFGFVYKNLLKPRGWMKPDVLDDNPEPHRFAQFLGFLFMTAGSVSLFLG
                     ATVLGWGLVWLVVALAALNAFGGFCVGCAVYYWLARFQLRGFNKQPPTGTFPGMKPKA
                     GVRA"
     gene            5714..6130
                     /locus_tag="VFQ23_22450"
     CDS             5714..6130
                     /locus_tag="VFQ23_22450"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9909425.1"
                     /translation="MSSDPMDIVLRFLLAIGIIGLGAAVYWYINYRLLLRAKNNIFTL
                     FNRLPKKPVIVYFTTPDCVPCKTVQRPALDQIRSLLGEKLQVVEINAYERPDLAQRWG
                     VMSVPTTFLLDASGEARYVNNGVARVEKLMEQVKTL"
     gene            6310..6582
                     /locus_tag="VFQ23_22455"
     CDS             6310..6582
                     /locus_tag="VFQ23_22455"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9909426.1"
                     /translation="MPVLRIPTPLRSYTSGQSEVNVSGGNISEALTDLTNQYPTIKPH
                     IFHENGDLRPFVNLFVGENNIKDLQGVETPIKDGDRLVLIPSIAGG"
     gene            6587..7738
                     /gene="moeB"
                     /locus_tag="VFQ23_22460"
     CDS             6587..7738
                     /gene="moeB"
                     /locus_tag="VFQ23_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET9909427.1"
                     /translation="MLPELSRDEILRYSRHLLIPEVGLEGQRKLKNSSALIIGTGGLG
                     SPVSLYLAAAGIGRIGLVDYDVVDSSNLQRQVIHGTSTIGKLKVDSARDKLIDLNPDI
                     QVDIFNEPYTSENALRIAADYDIILDGTDNFPTRYLTNDVAVFLGKPNVYASIYRFDG
                     QASVFYAKEGPCYRCLFPEPPPPGLVPSCAEGGVLGVLPGTIGTIQATEALKVLLGIG
                     EPLIGKLLLYNALDMSFDFVKLKKNPNCRVCGPNADIKELIDYEEFCGVPSHDHEEGS
                     AGAGLDITVQELAERVKTNHLRLLDVREPHELEISALPNATNIPLGQLAARLSELDTA
                     DEMVIFCKSGGRSARGLELLASAGFKKVKNLKGGINAWAKEVDPSLPLY"
     gene            7807..8943
                     /locus_tag="VFQ23_22465"
     CDS             7807..8943
                     /locus_tag="VFQ23_22465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9909428.1"
                     /translation="MKRVLILGGGFGGVATAHRLKERLGDQVEVILVDRRPFFMVGFR
                     KSWALVGESTLEDGQQPLDGLTRLGVRVMQDSVTRIDPQARSATMGDQRIEADALVVA
                     LGAELVPTAIPGFKEYALNVYDPNEIPRATQALDEFQGGRLVIGIFGAPYKCPPAPLE
                     MALLIQDKFKKKEVDASIEVFTPQPVTLPILSQESCDAVDGRLLEHGIRTYGHHKALR
                     VEAGEVIFEKRNIAFDLLLGIPPHRAPAVVRESGLTGESGWIEINPRTCATSFENVYA
                     IGDTTQIVLSNKKTLPKAGLFAELMGETVADRLAAEFSSQEPTAQFSGTGGCYFETGN
                     GEAMMIKGDFLAEPAPLVDITESSKEYYDEKVRFETDRWARWFG"
     gene            complement(9049..9636)
                     /locus_tag="VFQ23_22470"
     CDS             complement(9049..9636)
                     /locus_tag="VFQ23_22470"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9909429.1"
                     /translation="MTRTDRRRQRTHKSLQNALIELISERDFDTITIQDIADRADVGR
                     TTFYLHYNSKEELFLSCHEAIVSRLQLGLFHPLSREEFLSSETHPGITSAYRHLEKDR
                     GSLYPIFQGKNSQLMLRWIRDRSSLEIEENLRATFKNVDSKVPLDILASYLAGAQIAL
                     MQWWLEKRRVYSPNDLAQALHQLQRAVIQSAFPEV"
     gene            9818..9973
                     /locus_tag="VFQ23_22475"
     CDS             9818..9973
                     /locus_tag="VFQ23_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909430.1"
                     /translation="MSQSPVKQDRGTPPPMPNWVKVFGAILIVLILAVIILHVTGNSL
                     GGLHHMP"
     gene            9998..10531
                     /locus_tag="VFQ23_22480"
     CDS             9998..10531
                     /locus_tag="VFQ23_22480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909431.1"
                     /translation="MKPNLRKFVRTVHVIFTVGWLGTVVGFLALAIVGLTNQEPETVG
                     AVYIAMALMTRFVIVPLSLAPLLITGPLLSVGTPWGLFRHYWILAKLIINILSTLILL
                     VHVQPIDYLARVAMEGTLSAADRGVQIQLLVAAGAGLMALLIATALAVFKPRGMTSYG
                     WRKQFQDNKVSPNESVS"
     gene            complement(10618..11277)
                     /locus_tag="VFQ23_22485"
     CDS             complement(10618..11277)
                     /locus_tag="VFQ23_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909432.1"
                     /translation="MFAGHLAVGLVLKKVERRISLAWLFFATLFHDLLLGVFTLLGLE
                     KIIIPANYAQGHFLMFEFPYSHSLAASVVWSLLGFLLTYALLAKWANKERMQAGLAIA
                     LSVFSHFVLDWLVHVPDLPLFGADSPKLGLGLWENQPLALTLETALVAIGFIYYLTSI
                     KPQSNLARYGLASLLLFTTGMTVMGMLFATTPPPASGAAMSWILQPFLICGLAYWFDK
                     P"
     gene            complement(11334..11951)
                     /locus_tag="VFQ23_22490"
     CDS             complement(11334..11951)
                     /locus_tag="VFQ23_22490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9909433.1"
                     /translation="MATHPLEKTMIEQAQAGDLDAFNQLVLHYQDGLYHYAVSLVNDP
                     DLAEDITQESLIKAFQNIKNLQGYSFRAWLFRIITNTARDLARRSARHPLVSLYPKDD
                     NGEEVESPEWMIDPTSNVEEIVQGNEFSAQLYELLEELPEAYRNILILVDVQEMDYIE
                     AAEILNIPLGTVKSRLARARMQMKSKLQIMDKSRVFARGARALAV"
     gene            complement(12112..12627)
                     /locus_tag="VFQ23_22495"
     CDS             complement(12112..12627)
                     /locus_tag="VFQ23_22495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909434.1"
                     /translation="MQLSQLIQLGFFWGAIFGLVFCLATLIIGRINVVMLLNDYPPDI
                     RAKYGPMNEATRKQANKASLPLLVSLGFVLVLGLSQLRSLSGELTFINTLIVTLLIFQ
                     TWNLIDLIVLDWFLLMTLKPTFMIIPGTAGMAGYRDYMYHFRKFLNGIVFTVILSMVV
                     TLISLGTEWLI"
     gene            complement(12669..12986)
                     /locus_tag="VFQ23_22500"
     CDS             complement(12669..12986)
                     /locus_tag="VFQ23_22500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909435.1"
                     /translation="MKDNVWSVLGEFTNSSISENLNDPSNSLIKAMDPLHLPDSFLQR
                     LMAALQATMNDGIGRPSPATQKVRILSNYLDVPVQGSSSSWGFFFIEKDQEIELYLFQ
                     DRP"
     gene            complement(13004..13648)
                     /locus_tag="VFQ23_22505"
     CDS             complement(13004..13648)
                     /locus_tag="VFQ23_22505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9909436.1"
                     /translation="MKNKKRFLIISSILLLPFTYVLFQLTTAYLGPKWGYVSGFAGYW
                     AYCLATALLVAGADMTYFQRMWNGQPGRSTTKWLSLLAFVPVFGVFFVSFLPNAAALT
                     LPTGILLVIVVLLNGSIEELYWRGLYLLEYPDNTRIGFLFSWVLFGAWHISLWFARGV
                     IYEDGFLALVGGAYGLGLIWTWVARTNGNLRAVVPSHILVNLFAFTALFVSNGF"
     gene            complement(13718..15115)
                     /locus_tag="VFQ23_22510"
     CDS             complement(13718..15115)
                     /locus_tag="VFQ23_22510"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9909437.1"
                     /translation="MSTTLETNELKSIDELRQAVKGTVLTADDAGYDAARQVWNLAVE
                     QHPAVIVIAQSAEDIAEAVGFANANDLDIAVKATGHGTIREANGSLLIVTAHMNDVQI
                     DAAAQTAWISAGTKWGRVLEQAQAVGLAPLLGSSPDVGAVGYTLGGGMGWLARHYGLS
                     IDNVNYFEVVTAQGEIVTASSTENSDLFWALRGGGGNFAVVTGMEVRLVPVKEVYAGN
                     LYYPAELAPEVFARFETWAANAPDELTSSVVLMNFPPIPEMPEFMRGQSFVMVRGCYS
                     GRVEDGEALVKYWRDWQSPVVDDFKVMPFSQAATISNDPVDPTPGQSSGAWLKDLGAE
                     TAETLIHFVLPHGAPPKLVMAEVRHAGGQISKIDPGSTAYGNRDAQFSLQVVGFTPTP
                     QSVLEVREHIANMKQALEPHMHGGVYLNFLEGAEARQRTRQGFSAENFKCLQDIKAKY
                     DPQNRFNHSYVFASK"
     gene            15219..17756
                     /locus_tag="VFQ23_22515"
     CDS             15219..17756
                     /locus_tag="VFQ23_22515"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9909438.1"
                     /translation="MDEAPYSFGEWVRRRRKALDFTQQELADRAGCSLPALQKIEREE
                     RRPSRQLAALLADQLQISPEQREKFIRVARGERTADTLPSTLEPVSPPQAIASAAISY
                     PPLPVPATPLIGRENELETIRRLLADPACRLVTLAGPGGIGKTRLALEIAHLLQNEFA
                     DGVYFAQLVGVKGTEFIVPSLAEAVGFTFSGAKSPNVQLQNFLRGKRMLIVTDNFEHL
                     LAGADLLNELLQHAPELRFLVTSREPLHLQAEWTLEVQGLPVPEESSDTNSSAAQLFL
                     LRARHVRVGFQSSDEDAKAIANICRLVGGVPLGIELAAAWVRVMSLNEILNEIQQNLH
                     FLTSTARDIPERHRSLRTVFDYSWRLLTEEEQTTLARLSVFRGGFTREASQKVTGASL
                     PTLLALIDKSLVRRAENDRFDLHELVRQYAAEHLADPESVQAAHAETYLNLWRDNDAL
                     WSTAVHFGRRRLLSADLDNFRVAWDWAVAHEKFEWQSNVFRAIWGFHEIHGSYAELRS
                     QMESAAQMARGKDPRVYGRALTYQAWANLRYGQIADAQATLDEALTVLRPLEDPTILF
                     EPIFFKGILYFISGDWAATQHWLAEARAQAVASGVPRFIAQSTVHQSGVLAMMHNDEE
                     SFDRLQAALERLKELDLMRAYLTAFNTYTVPVALRLGRLPELYQPLKEALDTYQQIGD
                     PWMVAVIHSNLGTVLAAMGEMDEARSHAAQSLLLFKQLGSQADIAFTHLRYGRIELVA
                     KDFSEAEYHFRESIRIGQSAQAFMAVADSAAELAKLWAADEKTTTQAMLLCTYLIKQP
                     TTRADLRAAAEALRVQLEAKLSPSNIEAIQTKLNAIPAESLLRELVG"
     gene            complement(17906..>18097)
                     /locus_tag="VFQ23_22520"
     CDS             complement(17906..>18097)
                     /locus_tag="VFQ23_22520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909439.1"
                     /translation="MAHLRAGRSNQALNAYQHGLDFTDALNELDDSIEDLEKLKFKKP
                     DLDGLEEILLMLKNWKPTK"
BASE COUNT         4374 a         4522 c         4744 g         4457 t
ORIGIN      
        1 tgaacacaag ctgggtcaat gtgacgcggt ttgccgggcg gtcctggtaa tgagcggtga
       61 cgggaacggt tggctctgat acggtcacag gagagccagg gaataacgaa gccgtatgac
      121 cgtcttctcc catccctaaa tacacaaggt caaagcgcgg ccaatcgaaa ggaggggcgg
      181 cgaattcttt cagtttggat gaataatctt tcgaagcctc aactggcgga agttcgcctt
      241 tgatacgatg gatgtttgac tctggagttg acacatgtct caataaaagt tctctcgcct
      301 gaccgtaact gctgcccgcc tcatctggcg gcacacagcg ttcatctccc caaaagactt
      361 gaactttgcc ccaatccact tgatcgcgat attcggttcc cagcaactga aagaggcgat
      421 tcggcgtact gcccccatta agtgccacca aaaattgatt tcgctcccga accgatcgcc
      481 ccgcctgttc cacgaaaaga ttcgcggcgt gccggctgag cctttccaac tcatggaaga
      541 tacgaagttc agctttcatg tttatggttt ctgaaacaag aaccaggcag attttcgatc
      601 gcgtttatca tccacgccat cgcggcgcga aacgagagtc agttgggcgg agataaggtc
      661 caggtcggcg ggatcaatgc ccgttcccgc gagatccggg tcctgtttta agaatccgta
      721 cataagccag aagccgtgtg gggcaaggat tcgattcagt tgcttcaggt agtttgcctt
      781 gccatctttg tgaatgctgt ggaaacaacc aatatccagt gcgagttcgt aaggacctgt
      841 tactcccttt agctgggtgg cgtcactgac gcttacctct gcctcgatgc tgtttgattc
      901 caattttttt cttgcgaggc caatcgcgcg cggtgcaaaa tccactcctg taacgcgcca
      961 acctgctttt gccaatgtga tcacattcgt gcctgtgcca catccaatgt caatggcgcg
     1021 agccgcttta tgattttcga taaagtctaa gagttcgggt gggctaatac cggtatccca
     1081 tttcggctga ccgaaatacc agtattggaa gaggatgcgg cgaaacagat tttgcattga
     1141 tataaatcga ccccgacggt ttcaggtaaa tttattgcaa agcttgtata aaaaagcctt
     1201 gtcaagcgct tccttatttg caagaaaaac cgttagggtc ttaacaccaa ctttgacaat
     1261 tcaattgagt tcttttacaa tcatgcgact tacatatccc aactgtatag gaagggtctt
     1321 ttcatgaaag agtataccac cgagtttatt cgcaacatcg cgctggtctc gcatggaggt
     1381 gccgggaaaa cgatgttggc ggaggctttt ttacatgcca cgggggctac cacgcgtctt
     1441 gggaaagtgg aagatgggac tgctatttca gattacgatg aagaggaaca ccgtcgaaag
     1501 atttcaattt attcaagcgt gattcctctg gagcaccggg atcataagat aaatattatt
     1561 gacgcgcccg ggtacactga ttttcagggc gaggcaattg ccgccctcag tgttgcggat
     1621 ggcgcgatta ttttggtgga tgccgtagcc ggggtggagg tcggtaccga gatcgcctgg
     1681 cgttatgcag atgaatttaa ccttcctcgg ttttttctca tcaacaagat ggatcgtgat
     1741 aatgccgatt ttgaaaagac ctacgctgcg ctggaggagt tcgtccgtgc acacggtaaa
     1801 cgggctttaa aagtgcattt gccgatcggc gagaagcaga acttcaaggg ggtggtggat
     1861 attatcggga tgaaatccta tttgggagat ggcaagacaa ccggtgagat tcccgcagaa
     1921 cttaaagaag aagcagaagc ggcacacttt accctcgtcg aagccgcggc agaaggggaa
     1981 gatgaattga tggagaaata tttggagaac ggctcccttt cagacgaaga aatggtacgc
     2041 ggattggaag atgtagtcta cgctgggacg tttgtcccga tcttctgcgc ggcaggcgcc
     2101 cgcgaggttg gttcgattgc cctgctcaat gacatcattg atctcatgcc ttccccactg
     2161 catggacctg cgcgtactgc aaaaggcaag gatggttctg aagaaaaatt aagcgcatct
     2221 gactccgctc cgttggcggc ttatgtatgg aagacgaccg cggatccatt tgttggcaaa
     2281 atgacctact tccgcgtatt ctccggttcg attcaagcgg acgcgcgggt atggaaccag
     2341 aacaaaaacg cggaagaacg tatgtcaggt ttgcatttcc aacgagggaa ggaacacatt
     2401 cctgctaaag ttgtccatgc gggcgatatc gggtgtgtct ccaagctgac cgtgtcctcc
     2461 accggtgaca ccttctgcga taaaggtcat ccgcttacaa tcgaaaaacc aaatttcccc
     2521 gccgcgttat accgcgtagc ggttactcct aaaacacaag ccgatgcggc gaagatttct
     2581 cctaccctca cccgcctgtg tgaagaagat atgactctct cgtgggaaaa cgataagatc
     2641 actcatgaaa ctgtcttgaa gggtatgggc gatcaacaca ttgatgtcgc tattcatcgt
     2701 gcacagacaa aattccaggt gggactggcg actcatgagc cgaagatccc ttatcgcgag
     2761 ggaatcacac gcaaagccaa tgcacaatat cgccataaaa agcaatctgg tggtgcgggc
     2821 cagttcggcg aagtccatct ccgtattgag ccctatcacg atggcgattt cgattttggc
     2881 gatgaactcg ttgggatgaa tctctccaag tcgtatttgc cgccgattga gaaaggcatt
     2941 aaagccacca tggaacgcgg cgcctttgca ggttacccga tgagtaatgt gaaagtgatc
     3001 gtatacgacg gtaaggaaca cgaagtggac tcaaaaccgg tcgcttttga aatcgccgga
     3061 cgcgaggcat tcaaactcgc ggtgcaggat gccggtcccg tgttgttcga gccgatcatg
     3121 aatgtgcgcg tgaccgtccc cgatgccagc atgggtgacg tcatgagcga cctcaatacc
     3181 cgccgtgggc gcgtccaggg cacagagtct gaacgaggca gtactgtcat tatggcgcat
     3241 gtacccatgt ctgagatgac gcgttacacg acccaactcc gttccatcac aggcgggcgc
     3301 ggttatttca ccatggattt tgatcactac gatgttgttc caacccatat tgccggtacg
     3361 atcatcgaag cacataagag agaaatggaa gcgaagaaag aagagtagtt tctctaccag
     3421 cgctatttct agatctcctc gaagaaaatt cgaggagatt tacgttaata aaagcaacct
     3481 aaattgtcat ttggatcgat gttttggtaa tttgtagttc atcggattct aattttcccg
     3541 ggttttctcc ttgacaattt gcgtaagaat cgtacaatac tcgtcatcat ttcagtagcc
     3601 atgattacat tcaactattg cttgtgtcta cggcggggct cgtaaccacc gcctgagacg
     3661 ctactcccgc agtcaacagc tcagaacggt ggacgactcc cgcgtgctct atatagcgag
     3721 gcgtttgttc gtcgttcttt tattttgtct gttgactgct tttgatttaa ggatgcaatg
     3781 acactttacg ataccagtct ttcccaaaca aatactgact ctcccacaga catcacgtcg
     3841 cgtgtcggga atacccctct tttatcgctt caacggattg gccgcgggct ctcgccgcgg
     3901 gtcaaggttt tcgcaaaggc agaatggttc aaccctggtg gctccgtcaa ggaccggcct
     3961 gcgcttaaca tcatccaaac tgcgctggca aacggtgaac ttggaaatgg caaacgcttg
     4021 cttgattcaa cttctggcaa tatgggaatc tcatacgcca catttggcgc agcgctcgga
     4081 atcccggtca cgttgaccat tcccgcgagc gccagccctg agagatttac aatgctgcgt
     4141 gcgctgggtg ctgaattgat caccactgat cccactgaag gctcggatgg tgcgattttg
     4201 gtggctcggc aactggctag cgaaagacca gacctatatt ggtatgcgcg tcagtacgat
     4261 aaccccgcca actggaaggc tcattttgaa agcaccgggc ctgaaatcct ctcacaaacc
     4321 aatggcgaag tgacacactt cgttgcgggt ctcgggactt cgggtacatt gaccggaacg
     4381 ggccgttatt tgcgtgagca actacctgat gtagcaatcc ttgcctttca gccggacgcc
     4441 gccttccacg gactggaggg gcttaagcac atgccaacgg ccatcaagcc cgggatttat
     4501 gatccattct ttgcggatga agtgcttgaa gtccgaacgg aagatgcgca tgaaatggtc
     4561 aggcggttgg cgcgtgagga aggtctgttt gtcgggatct cttccggggc ggcggcagtc
     4621 acggcattac gtgtagccaa cgagttggac gaaggaacgg ttgtcacagt ctttcccgac
     4681 gcgggctata agtatttgag tgataaggct ctgtgggagg gtaagtgaca ttgaacctca
     4741 cagatgaatt gttggcacgt attcatgctc atggggagga gtcctatccc gaggagggag
     4801 ctggctttct gcttggcgat gattcaggga acagccaacg tgccgttaca gaaattttcc
     4861 cgctcataaa ttcccgggag aatgaagcac gtcacaatcg gtacttgatc acgcctgaag
     4921 attacctcaa ggccgaatta gcagcagaca agcttggact gagtctgatc ggtgttttcc
     4981 attcacaccc agatcacccg aatcagccat ctgactatga tcgcgagtgg gcacagcctt
     5041 tcttttcata catcatcacc agtgtgaacg gaggcaaggc ggtagaaagc cgctcctggc
     5101 gtctgacaga agatcgttca aaatttgagg aagaagaatt gaaaatttct tagccactga
     5161 gcaaacagaa tttgtgcgtt attgatactg gaaatctctg tggcaatgat taggataacc
     5221 atatgacatc gcaaacttta caaaaagtag atcattctgc tctaaagaca aatcaaatca
     5281 ttatcatctc tttgaatata ctcgccttca ttcttaattt accgatcctc gcggccgttg
     5341 tcgcgttggt gatggggatt gggtctgcgt tgaaagttcc gggcttcgga tttgtttata
     5401 agaacctgct caagccgcgc ggctggatga aaccggatgt gctggacgat aaccctgagc
     5461 cgcatcgctt tgctcagttt ctgggctttc ttttcatgac agcaggttct gtttcgctat
     5521 tccttggcgc tacggtgtta ggctggggcc tggtttggct ggtggttgca ctcgcggcgt
     5581 tgaatgcttt cggcggcttc tgcgttggtt gtgctgtgta ttattggttg gcgcgttttc
     5641 aactgcgcgg ttttaacaaa caaccgccca ctggcacttt ccctggaatg aaaccaaaag
     5701 ctggagtgcg tgcatgagtt ccgatccgat ggacatcgtg ttgcgttttc tcctcgccat
     5761 cggtatcatt gggttaggcg ccgcggttta ttggtatatt aattaccgcc tgttgctgcg
     5821 tgcaaagaat aatattttca cgttattcaa caggctgccg aagaaacctg tcattgttta
     5881 cttcacaacg cctgattgcg ttccctgcaa aaccgtccag cgacctgcgc ttgatcagat
     5941 tcggtctttg ctcggcgaga aattgcaggt ggtcgaaatc aacgcctatg aacgaccgga
     6001 ccttgcccaa cgctggggtg tgatgagcgt cccgactaca tttttgctgg acgcgagcgg
     6061 tgaagcacgt tatgtcaata atggcgtggc acgcgttgag aaattaatgg agcaggtaaa
     6121 gactttgtaa tgatttatcc gttggtcgag tagccgcgcc gattgttggt gccacttatc
     6181 gcgaccaacc agtaacatga aataaagaat aacttgaaac gggtggtttc gactgctcac
     6241 ctgcactgcg cgcagtgcgc aagtgtgcgc tcaaccaccg catctataaa taatttgagg
     6301 agaattttta tgcccgtatt acgaatccca acaccgttgc gttcctatac cagtggacaa
     6361 agcgaagtga acgttagcgg cgggaatatt tccgaagcgc ttaccgatct aacgaatcag
     6421 tatcccacga tcaaaccaca tatcttccac gagaatggtg atttgcgtcc ctttgtaaac
     6481 ctgtttgttg gcgaaaacaa tatcaaggac ctgcagggag tggaaacgcc gatcaaggat
     6541 ggagatcggc ttgtattgat tccatccatt gcaggaggct agatagatgt tacctgaact
     6601 ttcacgtgat gaaattctac gttactcgcg tcatttgttg attcccgaag tagggctgga
     6661 aggtcaacgc aaattaaaaa attcatctgc actcatcatt ggaaccggtg ggcttggctc
     6721 gcctgtttca ctttatttgg cagccgccgg catcggtcga atcggactcg ttgattacga
     6781 tgttgtcgat tcatccaacc tgcagcgaca ggtcattcat ggaacgtcca ccattggtaa
     6841 gctgaaagtg gacagcgccc gggataaatt gattgatcta aacccggata tccaggtgga
     6901 tatttttaac gagccttaca cgtcagaaaa cgcgctgcgc atcgcggcgg actatgacat
     6961 catcctcgat ggcacggata acttcccaac tcgttatctc accaacgatg tggctgtgtt
     7021 ccttggcaag ccgaatgtgt acgcctcgat ctatcgcttt gatgggcagg caagtgtgtt
     7081 ctatgccaaa gaaggacctt gctatcgctg tctcttcccc gagccaccgc caccgggact
     7141 tgtcccatcc tgcgcagaag gtggagttct gggcgtatta ccaggtacga ttggaacgat
     7201 ccaggcgaca gaagccttga aagttctgtt gggaatcggc gagccgttga tcggcaaatt
     7261 actgctctac aatgcgcttg acatgagctt tgattttgtg aagctaaaga agaatccaaa
     7321 ctgccgtgtc tgtggtccga atgctgatat caaagaattg attgactatg aagagttttg
     7381 cggcgttcca agccatgacc acgaagaagg gtcagcgggt gctgggttgg atattactgt
     7441 gcaggaattg gcagagcgcg tcaagacgaa tcatctcagg ctattagacg tgcgcgaacc
     7501 acacgagttg gaaatctctg cgttgcccaa cgcgaccaac atccctcttg gtcaattagc
     7561 agcacggctt tccgaattgg ataccgcgga tgaaatggtg atcttctgta aatcgggtgg
     7621 tcgctcggcg cgtggcttgg agctgctcgc cagcgcgggt ttcaaaaagg tgaagaacct
     7681 caaaggcggc atcaacgcct gggcaaaaga agtagaccca agtttgccgt tgtattaaat
     7741 tgataagcaa tcccgtaggg gtgggcagct gcccacccct acgggattaa agaggagaga
     7801 gggtatatga aacgtgtctt gattctcggc ggtggatttg gcggtgttgc gacagcgcac
     7861 cggcttaaag aaagactcgg agatcaggtc gaagtgattc ttgtggatcg ccgtccgttt
     7921 tttatggtgg gctttcgcaa gagttgggcg ctcgtggggg aatcgacgct tgaagatggt
     7981 caacagccgc tcgacggcct caccaggcta ggcgtccgcg tgatgcaaga ttccgtaaca
     8041 agaattgatc cgcaagcgcg ttcagcaacg atgggagatc aacgaattga ggctgatgca
     8101 ttggtagtcg cgctgggtgc tgaacttgtt ccaactgcta ttcctggatt caaagaatat
     8161 gctctcaatg tgtatgaccc caatgagatc ccgcgtgcta cgcaggcgtt ggatgagttt
     8221 caaggtggtc gattagtgat cgggattttt ggcgcgccgt ataaatgccc cccggctcct
     8281 ctggagatgg cgttgctgat tcaggataaa ttcaagaaaa aagaggttga cgccagcatc
     8341 gaagttttca cgccacagcc agtgacattg cccattctga gtcaggaaag ctgcgacgcg
     8401 gttgatggca ggctcctgga acatggaatt cgaacgtacg gacatcataa agcgttgaga
     8461 gttgaggcag gagaagtgat tttcgagaaa cgaaatatcg cctttgatct gctactgggt
     8521 atcccgccac atcgagcgcc tgcggttgtg cgcgagagcg ggctgacggg cgaatccggc
     8581 tggattgaga tcaacccgcg cacgtgcgca acatcctttg aaaatgtgta tgccataggc
     8641 gatacaaccc agatcgttct ttccaacaaa aagactctgc ccaaagcggg cctgttcgct
     8701 gaactgatgg gcgagaccgt ggctgaccgg ctcgcggctg aatttagtag tcaggaacct
     8761 actgcccaat tctcaggtac tggcggttgt tactttgaaa cgggcaacgg cgaagccatg
     8821 atgatcaaag gtgactttct ggcggaacct gccccgctcg tcgacatcac cgaatcatcg
     8881 aaggaatact atgatgagaa ggtccggttc gagactgatc gttgggcgag gtggtttgga
     8941 taggcaaagg atgaagcatg aatggtctcc cgtcggagta ttggcgggag actgtatttt
     9001 aaattgtctt cgtggaaaag ctggtatttt gatggaagcc gattcaattc aaacttcagg
     9061 gaatgcagat tgtatgactg cacgttgtag ctgatgtagt gcctgtgcca ggtcgttcgg
     9121 cgaatatacc cggcgctttt ccagccacca ttgcatgagt gcgatttgtg cgcctgcgag
     9181 gtagctcgcc agaatgtcaa gtgggacctt gctatcaacg tttttaaagg tcgcgcgcag
     9241 gttttcctcg atctccagtg agcttcgatc gcgaatccat cgtagcatca attgactgtt
     9301 cttgccttga aagatcggat acaacgaccc tctatccttt tcaaggtgtc ggtatgccga
     9361 tgtaatccct ggatgtgttt cggatgatag gaattcctct cgggaaagcg ggtggaataa
     9421 cccaagctgt aagcggctta cgatggcttc atggcagctc agaaataatt cctctttgct
     9481 gttgtagtgc agatagaagg tggtccgtcc aacatcggcg cggtctgcaa tatcctgaat
     9541 cgtaatcgta tcaaagtcgc gttcacttat cagctcaatc aatgcattct gtagtgattt
     9601 gtgtgtacgt tgtcttcgtc gatctgttct tgtcataata aatcctcggc atggataatt
     9661 gtactctcag tgggcgaatg gtgaggcgct cttcaagatt ctgaacagtt gtccgttatt
     9721 gttcacaaat agacaaatct tattctttga ttattgaaat tgacaggcgt tcacgatatt
     9781 ctgtatgcga aatctaaaac cgttcaaagg ataagtcatg tcacaaagcc ctgtaaaaca
     9841 agaccgcgga acgcctcccc cgatgccgaa ctgggtgaag gtgtttggag ccattctgat
     9901 cgtcttaatt ttggcagtaa ttatcctgca tgtcacgggc aatagcctcg gtggactgca
     9961 tcacatgcct taaggtttga aagtagcccc gattgttatg aaacccaatc tccgtaaatt
    10021 tgtgcggact gtacatgtaa ttttcacagt gggctggctg ggcacagtcg ttggttttct
    10081 ggcgcttgcc atagttggtt taaccaatca agagccagaa acagttggtg cagtctatat
    10141 tgccatggca ttgatgacaa ggttcgtaat tgtacctttg agtctggctc ccctgctcat
    10201 caccggaccg ctcttatccg tgggtacacc atggggatta tttcgtcatt actggatttt
    10261 ggcgaaactc attatcaaca tactgtccac ccttatcctg cttgtgcatg ttcagcccat
    10321 tgattatctt gctcgtgttg cgatggaagg tacgttatct gcggctgatc gtggagtaca
    10381 aattcaattg ctggttgccg ctggggcggg tttaatggca ttgctcattg caaccgcttt
    10441 ggcagtcttc aagcctcgag ggatgacttc ctatgggtgg cgtaaacaat ttcaggataa
    10501 taaagtttca ccgaatgagt ccgtttctta agatgtgtgt aagaaatcat gtgatgagaa
    10561 cagtagtttt acagcctacc gtcaaagttt tggcccgaga ttttttatgc gaatacacta
    10621 tggcttatca aaccaatatg caagaccgca gataaggaag ggttgcagta tccatgacat
    10681 ggctgcaccg gatgccggcg gtggagtggt ggcaaagagc attcccatca cagtcattcc
    10741 tgttgtaaat agcagaaggc ttgccaaccc gtagcgtgcg agattcgatt ggggtttgat
    10801 cgaagttaga tagtagataa agccaattgc aaccagggca gtttcaaggg taagggcgag
    10861 cggctggttt tcccataagc ccagaccaag cttcggcgaa tccgccccga acaggggcag
    10921 atccgggacg tggacaagcc aatccaaaac aaagtgcgaa aagacactca gcgcaatcgc
    10981 cagaccggcc tgcatccgtt ctttgtttgc ccatttggct aggagggcgt acgtgagcag
    11041 gaacccaagc agagaccaaa caaccgacgc agcgagtgaa tgtgaatagg gaaattcgaa
    11101 catcaggaaa tgtccctggg cataattcgc agggatgatg atcttctcga ggcccagcag
    11161 ggtgaaaact cccaatagaa gatcatggaa gagggtggcg aagaacaacc aggctaggct
    11221 gatgcggcgt tctacttttt tgagaactaa accgacggct aaatgtccag cgaacataag
    11281 tatctccttg ttgagtaact tttattgatt gctaccaggg aatgaaagca attctagact
    11341 gccagcgcac gtgcgcccct tgcgaagaca cggcttttgt ccataatctg gagttttgac
    11401 ttcatttgca tccgcgcccg tgcgagtcga cttttgactg tacccagagg gatattgaga
    11461 atctcggcgg cttcgatata gtccatttcc tgcacgtcaa ccaggatcag gatgttccga
    11521 taagcttccg gcaattcctc caatagttca tacagttgcg cggagaattc atttccttgt
    11581 acgatctcct ccacattcga agtagggtca atcatccatt caggagattc gacctcttcg
    11641 ccattgtcat ctttgggata cagcgaaacg agagggtgcc ttgcactccg gcgagcaaga
    11701 tcgcgagcag tattggtgat gatcctgaag agccaggctc gaaacgaata tccctgcagg
    11761 tttttgatat tctgaaaagc tttgataaga ctttcctggg tgatatcctc agcaaggtcc
    11821 gggtcattga ccagcgacac ggcgtagtga tacaaaccat cctgataatg gagcacgagt
    11881 tggttgaagg cgtcgaggtc gccagcctgt gcttgttcga tcatggtttt ttccagcgga
    11941 tgcgttgcca tagtctctcc tcttacctgt tacacgatgt tgcgtctgtg atttcgatgt
    12001 ttctacttta tcggttttga tattgtgata ggtgtcgtaa agtggtacaa aaatcggtac
    12061 aaaaaaacac agccgttgtc gactgtgttt tttgcaaaaa cgaaatttat ttcagatgag
    12121 ccactccgtg cccaggctga tcagcgtgac caccatggac agtatcactg taaacacgat
    12181 tccattgagg aacttgcgga agtgatacat gtaatcgcgg tagcctgcca taccggcagt
    12241 gccagggatg atcataaagg tgggtttcaa ggtcattaga agaaaccaat ccaatacaat
    12301 aaggtcaatg agattccagg tttggaagat cagtagtgtg acaataaggg tgttgatgaa
    12361 ggtcagttcg ccagaaaggc tgcggagctg actcaaaccc agtacgagaa cgaacccgag
    12421 tgagacaagc agcggaagcg acgctttgtt agcttgtttg cgtgttgctt cgttcatagg
    12481 gccgtatttg gcgcggatgt cgggtgggta atcattgagc agcatgacga cgttgatacg
    12541 tccaatgatg agcgtggcaa gacaaaagac cagcccgaag atcgcgcccc agaagaaacc
    12601 aagttggatg agttgagaga gttgcatgtg acgctccttg aaatttagtt gatctttttg
    12661 atggtctgct acggtcggtc ttgaaacagg tagagctcga tctcctgatc tttctcgatg
    12721 aagaagaagc cccaactcga ggacgatcct tgtacgggta cgtccagata gttcgagagg
    12781 atgcggacct tctgtgttgc tggactaggt ctgccgatac cgtcattcat tgtcgcttgc
    12841 agcgcagcca tcaaacgttg aaggaatgaa tctggcagat gtaaaggatc cattgccttt
    12901 atcaagctgt ttgaggggtc gttcaaattt tcagagatgg atgagtttgt aaattcaccc
    12961 agcacactcc atacattgtc tttcattcaa tttcactttc agtttagaaa ccattgctca
    13021 caaacagggc agtgaaggca aatagattga ccagaatatg cgacggaaca actgcacgca
    13081 aatttccatt cgtgcgagcc acccaggtcc agatcagacc cagcccatac gcgccgccta
    13141 cgagagccag gaacccatcc tcatagatca cgccccgtgc aaaccacagg gaaatatgcc
    13201 aggccccaaa caacacccac gagaagagga agccgatgcg ggtgttatcg ggatattcaa
    13261 gcaggtacag ccctcgccag tacagctctt caatcgaacc gttcagcagg actacgatca
    13321 ccagcaaaat tccagtgggc aaggttaacg ctgcagcgtt gggtaggaag gagacaaaga
    13381 acacgccaaa aaccgggacg aaggccagga gcgaaagcca ttttgtggtg gatctgcccg
    13441 gctgtccatt ccacatgcgt tggaagtagg tcatgtccgc gcctgcgacg agcaaggcgg
    13501 tggccagaca gtatgcccag taccctgcaa agcctgaaac gtaaccccat ttgggcccga
    13561 gataggccgt ggtgagctgg aacagcacat aagtaaaggg tagtaaaaga atggatgaga
    13621 ttattaggaa gcgttttttg tttttcatct tttcacctgt gcgaattttg agggatggtt
    13681 gtttcgcaac cacccctcaa tcagaatacg ataaagttca cttgcttgca aacacgtaac
    13741 tgtgattgaa gcgattctgt gggtcgtact tggctttgat atcttgcaaa catttgaagt
    13801 tttcggcgct gaatccctgc cgcgtgcgtt gacgagcttc tgcgccttcg aggaaattca
    13861 agtacacacc gccgtgcatg tgtggttcca gggcctgctt catgttcgcg atatgctcac
    13921 ggacttccaa cacgctttgt ggagtgggtg tgaaacccac cacttgcaaa ctgaactggg
    13981 catcgcgatt gccatacgcc gtggaccccg gatctatttt ggaaatctgg ccgccggcat
    14041 ggcgaacttc tgccatgacc aacttgggag gcgcgccgtg cggcaatacg aaatgaatca
    14101 gcgtctcggc cgtttcagcg cccagatctt tgagccatgc gccactgctc tgacccggcg
    14161 tggggtctac agggtcgttg ctaatcgtcg ctgcctgtga aaagggcatg accttgaaat
    14221 catccaccac gggtgactgc cagtcgcgcc agtatttcac caacgcttcg ccatcttcca
    14281 cgcgacccga gtaacagccg cgcaccatca cgaacgattg accgcgcatg aactcaggca
    14341 tttcagggat tggcgggaag ttcatcaaga ccaccgatga agtcaactcg tccggggcat
    14401 tggcggccca agtctcaaag cgggcaaaca cttctggggc cagttcagcg ggataataca
    14461 aattacccgc ataaacttct ttgaccggta ccaggcgaac ttccatgccg gtaactaccg
    14521 caaagttccc tccgccgcca cgtagcgccc agaacaaatc ggaattttca gtggagctgg
    14581 cagtaacgat ctcgccttgg gcggtgacga cttcaaagta attcacatta tcaatggata
    14641 atccataatg ccgggccagc cagcccatcc cgccgcccag ggtgtagccc actgcgccga
    14701 cgtccggcga cgaacccagc agaggggcga gcccgaccgc ttgagcctgt tcgagcacgc
    14761 gtccccattt ggttcccgca ctgatccagg ccgtctgggc tgccgcgtcg atctgcacat
    14821 cgttcatgtg cgcggtgacg atcaacagac tgccattcgc ctcacggatc gtgccatgcc
    14881 cggtggcttt cacggcgatg tcgagatcgt tggcgttcgc aaaaccgacc gcctcggcaa
    14941 tatcctccgc cgattgcgcg atgacgatca cggctgggtg ctgctccacg gccaaattcc
    15001 atacctgtcg cgccgcatca tacccggcat cgtcagcggt cagcaccgtg cctttcactg
    15061 cctgacgcaa ttcatcaatc gatttgagtt cgttcgtttc aagcgttgtg ctcatgatta
    15121 actccttagg tttgatgttc gcattaaaca tccaaacctg ccataacagg ggctggttaa
    15181 ccgtacaaaa agcggtacaa aacaagtaca atacgcccat ggatgaagcc ccatactcgt
    15241 tcggcgagtg ggttcgtcgc cgccgcaaag ccctcgattt cacccaacaa gaacttgccg
    15301 accgtgccgg ctgttcactg cctgccttgc aaaagatcga acgcgaagaa cgtcgccctt
    15361 cccgtcaact cgccgcacta ctggcagatc aacttcagat ttctcccgag caacgtgaaa
    15421 aattcatccg agtcgcccgt ggcgagcgga cggctgacac gcttccttcc acgctcgaac
    15481 ctgtttcgcc cccccaagcc attgcctccg ccgccatttc atatccgcca ttgcccgttc
    15541 cagccacgcc gctgattgga cgcgaaaacg aactcgaaac catccgccgc ctgctggcgg
    15601 acccagcctg ccgactcgtc accctcgcgg gcccaggcgg gatcggcaaa acgcgtctcg
    15661 cgttggaaat tgctcatctc ctgcaaaacg aatttgccga cggtgtgtat tttgcgcaac
    15721 tggtcggcgt caaggggact gagttcatcg tccccagcct ggccgaggca gttggcttta
    15781 cgttctcagg ggccaaatct cccaacgttc aactgcaaaa ctttttgcgc ggcaaacgca
    15841 tgttgatcgt gaccgataat tttgaacatc tcctggctgg tgccgacttg ctgaacgagc
    15901 tcttacagca tgctcctgag ctcaggttcc tggtcacctc gcgtgaaccc ctgcacctgc
    15961 aagccgaatg gacgctcgaa gtgcaagggc tcccggtgcc cgaagaaagt tcggatacca
    16021 acagtagtgc tgcgcagtta tttctgctgc gggcgcgtca cgtgcgagtg ggctttcaat
    16081 cctccgatga agatgcgaaa gccattgcca atatctgccg cctggtgggt ggcgtgcctc
    16141 tgggaatcga actggccgct gcctgggtgc gggtcatgtc gttgaatgag atcttgaatg
    16201 agatccagca gaacctgcac tttctaacca gcactgcccg cgatattcct gagcgtcatc
    16261 gttccttgcg caccgttttt gactattcgt ggcggctgct gactgaagag gaacaaacta
    16321 cgctggcgcg cctctccgta tttcggggtg gcttcacgcg tgaggcttct caaaaagtaa
    16381 ccggcgcaag tctgcccaca ctgctagccc tcattgataa atccctggtt cggcgtgccg
    16441 agaacgaccg tttcgatttg cacgaactgg tgcgtcaata tgcggctgag caccttgctg
    16501 acccagagtc cgttcaagcc gcccacgccg agacatacct caacttgtgg cgcgataacg
    16561 atgcactgtg gtccaccgcg gttcatttcg gtcgccgccg gttgctcagc gccgatttgg
    16621 acaacttccg tgttgcctgg gactgggccg ttgcgcatga aaaattcgaa tggcagagca
    16681 acgtatttcg cgccatctgg ggctttcatg agatccatgg ctcttatgct gaactccgct
    16741 cgcagatgga gagtgccgca caaatggccc gtggaaagga tccacgtgta tacggtcgtg
    16801 cgctcaccta tcaggcctgg gcaaatttac gctacgggca aattgccgac gcccaggcaa
    16861 ccctcgatga agccctgact gttttgcgtc cgcttgagga tcccaccatc ctgttcgagc
    16921 cgatcttctt caaggggatc ttgtatttca tcagcggtga ttgggcggcc acccagcact
    16981 ggctggcgga ggcacgggcc caggcagttg cctcaggggt cccgcgtttt attgcgcaat
    17041 ccacggtaca ccaaagcggc gttttggcga tgatgcataa cgatgaagag tcgtttgatc
    17101 ggttgcaggc cgcccttgag cggcttaaag aactggactt gatgcgtgcc tacctgaccg
    17161 ctttcaacac gtacaccgtg ccggttgcct tgcggctcgg acgcctgccc gaactgtatc
    17221 aaccgctgaa agaagcgctg gacacctatc agcaaattgg tgatccctgg atggtggctg
    17281 taatccattc aaatcttgga accgtgctgg cggcgatggg agagatggac gaggcccgct
    17341 cacacgccgc ccaaagcctg cttctgttca aacaactggg ctcccaggca gacattgcct
    17401 tcacccacct ccgttacgga cggattgaac tcgtcgccaa agacttctcc gaggccgaat
    17461 accacttccg tgaatcgatt cgaatcgggc aaagcgctca ggcctttatg gcggtcgccg
    17521 attccgccgc cgaactggcg aaactctggg cagctgatga aaaaactaca acacaggcga
    17581 tgctcttgtg tacctatctt ataaaacaac ctaccacccg cgctgacctg cgggcggctg
    17641 cggaagcact ccgtgttcag cttgaagcaa agctctcacc atcgaatata gaggccattc
    17701 aaaccaaatt gaatgcaatc cctgccgagt ccctgctccg tgaacttgtt ggttgagcca
    17761 ggaacgctcc tgggtctcat actccattga agaaatacta cgccgacaaa atcacgctcg
    17821 agaccgatcg ctgggtgaag taatttgggt aagcaatgga tgaagcataa atgaaaaact
    17881 ctcgccgaaa cggcgagagt ttttactatt tggttggttt ccagtttttg agcatcaaca
    17941 atatttcctc tagcccatcc aaatcaggtt ttttgaattt taacttttca agatcttcta
    18001 ttgaatcatc cagttcatta agagcatccg tgaaatctag tccatgttgg tatgcattta
    18061 atgcctggtt tgagcgacct gcgcgcaaat gtgccat
//
LOCUS       DASTJP010000769         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_263522, whole genome shotgun sequence.
ACCESSION   DASTJP010000769 DASTJP010000000
VERSION     DASTJP010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263522"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFQ23_22525"
     CDS             <1..184
                     /locus_tag="VFQ23_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909440.1"
                     /translation="TKMRLSEKRAMEAASTGANVLAVACPFEVSRFEDAVKSTGNEHV
                     TVNDILELLDRSMRGS"
     gene            374..1021
                     /locus_tag="VFQ23_22530"
                     /pseudo
     CDS             374..1021
                     /locus_tag="VFQ23_22530"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1065..2012
                     /locus_tag="VFQ23_22535"
     CDS             1065..2012
                     /locus_tag="VFQ23_22535"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET9909441.1"
                     /translation="MSNDVFVITEQMDGVIAEVSFEMVGKAKELTTSWGGQVVAVVLG
                     SGVSADVFASNTTFVVDDPNLARFNPEAYGKIIEGLVNEKSPRLVMFGWTATGMDLAA
                     WLSARTGKPCVAYARDLCIENNSLVVNSQIYGGKMVAEVVPEGDGTIVACMAGSFPAE
                     AGRGSTAATKVDSPVSLEGLKTKFVEAIKPASGDVDITAQDKLISVGRGIGGRENIEL
                     AEELAEKLGAVVSASRPVVDAGWLPRTRQVGKSGLKVKPKLYLMLGISGAPEHLEGMK
                     SAELIIAVNTDKKAPIFNVAHYGATADLFEVAEAMLELL"
     gene            2190..>2666
                     /locus_tag="VFQ23_22540"
     CDS             2190..>2666
                     /locus_tag="VFQ23_22540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909442.1"
                     /translation="MLTTIEKIVFIILALISAGFTLHGIKTIVDSVRRARPAPELKNV
                     PATLIKAAITVLFQRTIFKARKILSAVHLGLFFGLVTYAFVNLVDVLEGFIPGFELVY
                     GGKHLAFPFLNSTVINIFNLIADLMSGALLVSITIFLVRRFIVKDKRLAYRENVLLN"
BASE COUNT          630 a          630 c          781 g          625 t
ORIGIN      
        1 cacaaaaatg cgcctctcgg aaaaacgcgc catggaagca gcttccacag gcgccaatgt
       61 gctggctgtg gcctgtccgt ttgaagtctc gcgttttgag gatgcggtca agtcgacagg
      121 caatgagcat gtgaccgtga atgatatttt ggagttgttg gatagatcga tgagaggtag
      181 ctaaaacacc cagagcgcca tcctcggcgc tcgataccgc cgccgaggac ggcggctgga
      241 gcatgaataa ggagagattc ttatgaacat agttgtcgca atcaaacaca ttcccagcat
      301 cgccgatgac ctgcctgtca aaggaaacaa cgtggacttt gactcggttg acttcgtata
      361 aaacgaattc gatgagcaat ccatcgagca ggcggtgctg gtgaaagaag ccgtgggagg
      421 gacggtaacc gtcgtcgggg tggatttgat tggggaactc gatagtgtac ttcatctcgc
      481 gctggcaaag ggcgcggata aggcggtcaa aatcacagcc gacgaaccag gagcagactc
      541 gcacatgcaa gccaaatggc tggcagaggc aatcaaaggc atgtcacctg atttggtttt
      601 cgcgggtgtg caagcttcca atgacctcga cggtcaaatc ggaccgatga tcgccgcgta
      661 tctcgatctg ccatatatcg gcggtgtaag ttccgtggaa gcctcggacg gctccgcaac
      721 agtgacaaag gaattcgccg gcggtatggc ggggaaatat tccatcagtt tgccgatggt
      781 agtcggcgtg caagccgcat ccaagccgcc gcgctatgct ccgatcagta aagtgcgtca
      841 agtcgcgcag accgcgacga ttgaaaaggt cagtccttct gcggatggag cgggggctgg
      901 tctcacgatg aggaagatgg ctccgcccgt cgtgaccggt cacgcggaga tgatcgaggg
      961 ttccgcgaaa gaggtcgcgg cgaagatcgt ggagttatta aagagcaggg gattagccta
     1021 gctgaccata gccgattcag agcatactaa ttacggagta aaacatgtca aatgatgttt
     1081 ttgtaattac agaacaaatg gacggcgtta ttgctgaggt ttcttttgaa atggtgggga
     1141 aggcaaaaga gcttactaca tcatggggcg gacaggttgt cgcggttgtc ttaggaagcg
     1201 gagtttccgc agatgtattt gcttctaata caactttcgt cgttgacgac ccgaacctgg
     1261 cgcggttcaa tcccgaagcc tatggcaaaa tcattgaagg attggtcaat gaaaaatccc
     1321 cacggctggt tatgtttggc tggacggcca caggcatgga cttagccgcg tggctttcgg
     1381 cgcgcacagg caagccttgt gtcgcctacg ccagagattt gtgcattgag aataactccc
     1441 tcgtggtgaa tagccaaatt tacggcggga agatggttgc agaggtcgtg cccgagggcg
     1501 atgggacaat cgtcgcctgt atggcgggtt cattcccggc tgaagcgggg cgcggctcga
     1561 ctgccgcgac gaaggtagat tcgccagtat cgcttgaggg cctgaaaacg aaatttgtag
     1621 aagccatcaa acctgccagc ggcgacgtgg acatcaccgc gcaagacaaa ctgatctctg
     1681 ttgggcgcgg gattggtggc agggaaaaca tcgaacttgc cgaggaactg gcggaaaaac
     1741 ttggggcagt ggtctcggct tcccgtcccg ttgtggatgc aggctggctg ccgcgcacgc
     1801 gccaggtcgg caagtcggga cttaaggtca aaccgaagtt gtatctcatg cttggcatct
     1861 ccggagcacc tgaacatctt gaaggaatga agtctgccga gttgattatc gcggtcaaca
     1921 ccgacaagaa agcgccgatc tttaacgtag cgcactacgg cgccaccgca gatttgtttg
     1981 aggtagccga agcgatgctg gagctattgt aatttcattg cgaggagcga aacgacgaca
     2041 ctggcaccgc acgtgaattg cttggccatt catgcaggtg agcaatctct gattagacgg
     2101 agggaactac ctggtgagtt ttgcctggtt taatggagat tgcttcgcta cgtgcgctcg
     2161 caatgacatg gtgtgctcgg gagggcactt tgcttacaac gattgaaaaa attgtcttca
     2221 tcattctggc gctgatttcg gcggggttca cgcttcatgg gatcaaaacc atcgttgatt
     2281 ctgtgcgcag ggcacgacct gcgcctgagt tgaagaatgt ccctgcgact ttgatcaaag
     2341 ctgcaattac ggttctcttt caacgcacca ttttcaaggc gcgcaagatc ttgagcgcgg
     2401 tccacctggg attgttcttc gggcttgtca cctatgcttt tgtgaatctg gttgacgtgc
     2461 tcgaaggttt catcccgggc ttcgagctgg tatatggcgg caaacatctt gcctttccat
     2521 ttttaaattc aaccgtaatc aatatcttca accttatcgc tgacttgatg agcggcgcgc
     2581 tcctcgtcag tatcacgata tttcttgtgc ggcgttttat tgtcaaggat aagcgcctcg
     2641 cttatcgcga aaatgttttg ttaaat
//
LOCUS       DASTJP010000770         8987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_913748, whole genome shotgun sequence.
ACCESSION   DASTJP010000770 DASTJP010000000
VERSION     DASTJP010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913748"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFQ23_22545"
     CDS             complement(<1..921)
                     /locus_tag="VFQ23_22545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9909443.1"
                     /translation="MSYLKLASYMLIRLVINTAYRMIYPFLSVFARGLGVEISVISGL
                     VANRAIAGAVEPFLFPFIETRGRKFGMLLGLGLFVLSMGLVAVWPNLTTFGIALVFSL
                     VGKSIFDPSLTSYVADHISYEIRGKAIGILEFAWSLAFIIGVPVAGFMIARASWSAPF
                     RVLAVLGFLAFIYIVFTLTDSEKPVHHADGIFGNVKQIFTTPTVLIAFSIGLLVTAAN
                     EVVNLMFGVWLEDSFKLQIAALAGASAVIGLSELGGEGFVALFVDRIGKVRAAGYGIV
                     ANCLAALLLPIIGRTQLGALTGLFLFYITFE"
     gene            1035..1535
                     /locus_tag="VFQ23_22550"
     CDS             1035..1535
                     /locus_tag="VFQ23_22550"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9909444.1"
                     /translation="MNNARPSSNQNQPPASVALEALGIPHRIFTHEGSVTSFEQAASE
                     RGQRESQVVRSILFQIRSDEFLLVLVAGPAQIDWKKLRQLVGRSRIRMATEGEVLGIT
                     GYRVGTVSPFGLKNQVRVLIDTSVLNEEEVSIGSGVRNTAVIMNTTDLRHALGDAEIV
                     SLLEGA"
     gene            1562..3655
                     /locus_tag="VFQ23_22555"
     CDS             1562..3655
                     /locus_tag="VFQ23_22555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET9909445.1"
                     /translation="MFQRNQLRRLRSQLVLTFLLSALGIGVAIGLPVILLISRQASSH
                     TQLLLEQATSTTRVFLSSELSDLQNLALLTSQRPTLARLLSEPDPSTLEDYLNTLREG
                     ADLDLLLVCADGEEVKGIGENISMAELCQRNPQSAYEIFNSNDDLYLYTGVDWESSEG
                     PVYEIVAGKTLSRVLTELQNETGLVYFLVLQNEIIRSSDPLIQPTVDESLELQNQANQ
                     ASNLSLERRSVNINQHEYISSVLDLDPGLEAQLISALNIDDQITTQQNLVQTLTLTLF
                     FIILVAFGLGVWQSQRLSLPIVNLANIAAKFRQGNLDSPVSIQSSVTEINQLANTLED
                     ARVALQHSMEQLQAEKDWIEHLLNSIVEGTLTLDAHNRITFASAGVGKILEQEPNQII
                     GRKVDEIFLPAEAEIPFSSQLPTTGQQRRVSVKLMNGQERLLSISKADFVPPVASDSN
                     RALVIRDVSNEEYIHRLLGDFMANITHEFRTPLAALEASSELLLDNLQNLSQDEIKEL
                     LVALNLGIINLQTLIDNLIEAASIEAGRFKVSIQPVPFDVILSEAQEIIQPLVEKYGL
                     RLKSSPIAEPVQVLADRRRTVQILVNLLSNAIKYSPDNGLIQINHSIQNETLRVEVTD
                     EGRGVPLEQRNHLFRRFSHLNASNERAKQGVGLGLSVVKAIVEAQAGEVGVVDSPGGG
                     SSFWFTLPLANGEER"
     gene            3652..4353
                     /locus_tag="VFQ23_22560"
     CDS             3652..4353
                     /locus_tag="VFQ23_22560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909446.1"
                     /translation="MKVLVVDDDRVLADLVIFTLRRAGNEGILASDAASAVRRWADDK
                     PDLIILDVNLPGTTGLENGFAICKRIRSESDTPIILLTARGDESDIVHGLEAGADDYV
                     LKPFSPRQLMARVQAVMRRFHTTSTSASTPAKLSVDGLEFNPKLREVTLSDGTSASLT
                     PLESKLLESLMINAGQMLTVDDLISDIWGAEGGSSEMLRQVVRRLRSKIEPDSTDPYY
                     IQNLPGLGYAFRLKR"
     gene            4518..6437
                     /locus_tag="VFQ23_22565"
     CDS             4518..6437
                     /locus_tag="VFQ23_22565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9909447.1"
                     /translation="MSILLENVTKRYEQQIVVNNVSLEIKDGEFFVLLGSSGSGKTTV
                     LNIIAGLASAEQGRVLLHGRDVTNLPTQKRNIGFVFQNYALFEYMTVAENIEFGLQIR
                     KVHKKERQLKRDELLELVGLVGLGDRMPRQLSGGQQQRVALARALVLEPDVLLLDEPL
                     GALDAKIRVDLRRSLKNIQKQLGVAAILVTHDQEEAFDLADRIGVMSYGRLVEVGTPQ
                     ELYQRPKTEFVASFLGTANILLGQTEGERIQIGPHIFKLAKDATRFSTDGRVQVVFRP
                     EDLALVQTEEELDCTPLGEGTVTSLGFNGPTERIRLELPSIRGVRAIAPSVPFGSQNI
                     LIEATRSPDQASAFPLGVSDKAWVGIRRIHALSHPGLNFLVLTDGSLRSQSALSVGSY
                     LARMAHARMTLLGVGKDETLLDTYLQDARKQLGSGMASIEVRTDPLPAPIAITKIIEK
                     DPVDLAIVGWRPVEGTEIAEQILRHGDHNLLLAAHPGARIEKALVCVASGEPGKDDVM
                     FAGRLLRHLGASAKLFTVLTPAMNNEHQNRLAVRFVSGGVQTLEMFGVPTESELKVGD
                     PLTEISEEIKRGAFDLVVMGSPFPQKDGRILLHGLIEGVMKNAGDCSVLIVRSHHHKN
                     TIKQNWSESLYTYIN"
     gene            6451..7524
                     /locus_tag="VFQ23_22570"
     CDS             6451..7524
                     /locus_tag="VFQ23_22570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9909448.1"
                     /translation="MFTKFRKTLLISLLLISILVTGCSAGEPTEGAGTEPATGDEVKL
                     ILAAYTTPREAYRELIPIFQQQWKDKTGQTVTFEESYQGSGAQSRAVAEGFEADIVAL
                     SLEADVIRLEDAGLITQDWRSQPFGGIISTSVVAFAVREGNPKNIQDWADLAQADLEI
                     LTPNPKTSGGAMWNVLALYGAAQRGHVVGYSADDAGAQDLLLSVLQNVTVMDKAARDS
                     IVNFEKGIGDVAITYENEVFVGQQAGQTYELVLPTSTIQIDNPIAIVDTYADKHGTRE
                     VAQAFIDFLFTPEAQQIFAKHGLRSPDPEVAAATAEQYPPIEDLFTIADFGGWKEATP
                     KYFGDNGIFYQVFAQVQGIQISE"
     gene            7610..8473
                     /gene="cysT"
                     /locus_tag="VFQ23_22575"
     CDS             7610..8473
                     /gene="cysT"
                     /locus_tag="VFQ23_22575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HET9909449.1"
                     /translation="MNLSTTISSTNKTQRSFPWGQLGIRSIALAYLALLLIIPLLVIL
                     QDGLKEGPAELWRQITLPAAWHALKLTLWTSTIMTVINIIMGTLTAFVLVRYEFPGKT
                     ILNGIIDLPLAIPTLVTGVMLVILFGPQEALGTWLKNQFDISIIFAPPGIILALLFLT
                     FPFVVRSVQPVLLELDRSQEHAAATLGAGGWTIFRRIILPPLTLPVITGALLSFARAI
                     GEFGSIVVVAGNIPFRSQTAAVYVFGEVESENRLGASAMSIVMVGIAFSLMMIADYLR
                     KRSKSTKKENL"
     gene            8470..>8987
                     /locus_tag="VFQ23_22580"
     CDS             8470..>8987
                     /locus_tag="VFQ23_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909450.1"
                     /translation="MSITTSVRTSLAKTQSPVRSWGEKNKGKWVQWGLIGIVIAYVSI
                     LILAPLTALTLGAFEQGFGAIIESFNHSFVLTSFWNTLWISLVVVIIHAVFGTIVAWV
                     FVRHRFPGRNLINALVDMPFAISPVVAGYMLLLLFGRNGLLAPLVEATGIRVAFAFPG
                     MLLATLFVTFPF"
BASE COUNT         2270 a         2283 c         2326 g         2108 t
ORIGIN      
        1 ttcaaacgta atatagaaca ggaacaatcc ggtcagcgcg ccgagctgcg tgcggccgat
       61 gatgggcagt aaaagcgccg ccagacagtt ggcaacaata ccatagcctg ctgcgcgtac
      121 tttgccgata cgatctacaa atagcgctac gaatccctcc ccgcccaact cagacaaacc
      181 gataactgca gaggctccgg caagcgcggc aatctgcaat ttaaaagaat cctccagcca
      241 tacaccgaac atcaaattga cgacttcatt tgcggcggtc actaaaagcc cgattgagaa
      301 cgcgatcaaa acggtcggtg ttgtgaaaat ctgcttcacg ttgccaaaga tcccatccgc
      361 gtgatgaaca ggtttttcag aatcggtcag tgtaaagaca atataaatga aagcaagaaa
      421 ccccagaacg gccagcactc gaaacggtgc cgaccagctt gctcgcgcaa tcatgaagcc
      481 ggcaacgggg acaccaataa taaacgccag ggaccaggcg aattcaagaa tcccgattgc
      541 cttgcctcgt atttcataag agatatgatc tgccacatac gatgtcagcg atgggtcgaa
      601 gatggacttc cccaccagag aaaaaacgag ggcaatgcca aacgtagtaa ggttgggcca
      661 tacggcgacc agccccatcg aaagcacgaa caaaccgagc cccagcagca ttccaaactt
      721 gcgtccgcgc gtttcaatga acggaaagag aaacggctct accgcaccgg caatcgcccg
      781 gttggcgacc aggcctgaga tcacgctgat ctctacccca agcccgcgcg cgaagacaga
      841 gagaaatgga tagatcattc gatacgctgt gttaatcacc aggcgtatga gcatgtacga
      901 cgcgagtttc aaataactca tgaaattatg tttgcgaatt acacaatggg aatgtagtga
      961 tataaagcat tagcaaattg taccctacaa gaaaaaagca accctgatgc cgtattgtga
     1021 taggattcct aattatgaac aatgctcgtc cttcttccaa ccaaaatcaa ccacctgcca
     1081 gtgtcgcgtt agaagcgctg ggtattcccc atcgcatctt cactcatgag ggcagtgtga
     1141 ccagctttga gcaggcggca agtgaacgtg gtcaacgcga gtcacaggtg gttcgcagta
     1201 ttttgtttca aatccgatca gacgaattcc tgctggtatt ggtagcgggc cccgcgcaaa
     1261 tcgattggaa aaaattacgc cagctcgtcg ggcgttcgcg catacgaatg gcaaccgagg
     1321 gtgaagtgct gggcatcacg ggatatcgcg tagggactgt gagtccgttt gggctgaaga
     1381 atcaagtcag ggtcctgatc gatacgagtg ttctaaacga ggaagaggtg tcgattggtt
     1441 caggagtccg caataccgcg gtcataatga acactactga tctacgtcat gcactgggag
     1501 acgcagaaat cgtctctttg ttggaagggg cataaccttc ccggttgagc aaacggcaac
     1561 tatgtttcaa cgaaatcaac ttcgaagatt aagatcacaa ctggtcctta cctttttgct
     1621 cagcgcgctc ggtatcggcg ttgcgatcgg gctgccggtg atcctgctga tcagccgaca
     1681 ggcctcctcc catacccaac tcctgctcga acaggcaacc tccaccacac gtgttttcct
     1741 ctcaagtgaa ctttctgacc tgcaaaattt ggccttactc acctcgcagc gaccaactct
     1801 ggcgcgctta ctctcagaac cggacccttc cacgctggaa gattatctaa acacactgcg
     1861 cgaaggtgca gatcttgatt tgctactggt ttgtgcagat ggggaagaag tcaaaggaat
     1921 cggcgaaaat atttcaatgg ctgaactttg tcagcggaat ccacagtcgg cgtatgagat
     1981 ttttaactcg aatgatgatt tgtatcttta caccggggtt gattgggagt catccgaagg
     2041 accagtctac gaaatagtgg ctggaaaaac cttgtctcga gtcttaacag aacttcaaaa
     2101 cgagacgggc ctcgtttatt ttttggttct gcaaaatgag atcattcgat ccagcgatcc
     2161 cttgatccag cccacggtgg atgagtcact agagctgcaa aaccaggcga accaagcatc
     2221 gaatctgtca ctggaacgac gctcagtaaa catcaatcag cacgaataca tttcttctgt
     2281 tcttgatctc gatccagggc ttgaggctca attaatcagc gccttgaata tcgatgatca
     2341 aataacgacg cagcaaaacc tggttcagac cttaacgcta acgttattct tcattatcct
     2401 ggttgcgttt gggttggggg tgtggcaatc tcagcggctc agcctgccaa ttgttaatct
     2461 cgcaaatatc gcggcaaaat ttcgacaggg gaatttggat tcgcctgttt ccatacaatc
     2521 ttcagtcact gagatcaatc aacttgccaa caccctggaa gatgcacgcg ttgcactgca
     2581 gcattccatg gaacaactcc aggcagaaaa agactggatc gagcatttgc tgaattccat
     2641 tgttgaagga acgcttacgc tggatgccca caatcgtatt acatttgcca gtgcaggcgt
     2701 tggcaagatc cttgagcagg aaccgaatca aatcattgga cgaaaagtgg atgaaatctt
     2761 tctgccggcc gaagcggaga ttccgtttag cagccagctc cctaccacag gtcaacagcg
     2821 gcgcgtctcg gtcaaattga tgaacggaca ggagaggttg ttatccatct ccaaggccga
     2881 ttttgtccct ccggtggcca gtgactcgaa tcgtgcgctg gtgattcgcg atgtatccaa
     2941 tgaagaatac attcatcggc tgctgggcga ctttatggcg aatatcacac atgagttccg
     3001 cacacccctg gctgccctgg aagcttcttc tgagctcttg ctggataacc ttcagaacct
     3061 ctcgcaggat gaaattaagg aactcctggt ggcgttgaat ctggggatta tcaatttgca
     3121 aactctgata gataacttaa ttgaagccgc aagcattgaa gcaggacgct tcaaagtttc
     3181 cattcaacca gttccatttg atgtcatttt gagcgaggca caggagatca tccagccgtt
     3241 ggtcgaaaaa tatgggctaa ggttgaaatc ctctccaatc gcagaacccg tccaggtgtt
     3301 ggcagaccgc aggcggacgg tgcaaatcct cgtcaattta ctttccaatg caattaaata
     3361 cagcccggat aatggcctga tccagattaa tcattccatc caaaatgaaa ccctgcgggt
     3421 cgaagtcacg gacgagggca ggggcgtccc actggagcag cgcaaccatc tcttcagacg
     3481 tttctctcat ctgaatgcct caaacgaacg cgccaaacaa ggcgtgggac tgggtctttc
     3541 ggttgtgaag gcaattgtcg aagctcaagc gggtgaagtt ggagttgtcg attcgccggg
     3601 tggcggcagt tctttctggt ttaccctgcc tctcgccaac ggagaggaac gatgaaagta
     3661 ttggttgtgg atgatgaccg agtccttgcg gacctggtca ttttcacact tcgccgcgcg
     3721 ggcaatgagg ggattcttgc cagtgacgct gccagtgccg tccgtcgctg ggcagatgat
     3781 aagcctgatt tgatcattct ggatgtcaat ctgccaggca caaccgggtt ggaaaatggc
     3841 tttgcaatct gcaaaagaat tcgcagtgaa tcagatacgc ctatcatctt actgacggcg
     3901 cggggcgatg agtctgatat tgtgcacggg ttagaagctg gcgcagatga ttatgttctg
     3961 aaaccattta gcccgcgaca gctgatggca cgcgtgcagg ccgtgatgag gcgatttcac
     4021 accaccagca cttccgcttc aacccctgcg aaattatcag tggatggatt ggagttcaat
     4081 cccaaactca gagaggtgac tctttctgat ggaacgtcag ccagcctcac tccgttggaa
     4141 agcaaactgc tcgaatcgct gatgatcaac gccggccaaa tgcttacggt ggatgacctg
     4201 atcagcgata tctggggagc agagggcggc agttcagaaa tgctccggca agtcgtgcgt
     4261 cgcctgaggt ccaagatcga gccggactct accgatccgt attacattca gaatttacca
     4321 gggttgggat acgcgtttcg attaaaacga taagtcattg gtcgaatagg gggcgatcgt
     4381 agccatatcg aaaccacaga caatcataaa aggagccggg ggaaatcaaa aattgtcaca
     4441 gcattgtcac acctctatca ccaggctcgt cacacctcat gggtatccta taagagataa
     4501 aaaatcctga ggtgaaaatg tcaattttgc ttgaaaatgt aacaaaacgc tatgaacagc
     4561 agatcgtcgt aaataacgtc tctttggaga taaaagacgg cgagttcttc gtattactgg
     4621 gctccagcgg aagtggaaaa acaactgtcc tcaacatcat cgccggtttg gcttctgctg
     4681 aacaggggcg cgttttatta catggacggg atgttacgaa ccttcccaca caaaaacgca
     4741 atatcggttt tgtatttcaa aattatgcac tttttgagta tatgaccgta gcagaaaata
     4801 tcgagttcgg actgcagatc cggaaagtgc ataaaaaaga acgccagctc aagcgagatg
     4861 aacttttgga attagtaggt ctggttggat taggcgacag aatgccgcgc cagctctctg
     4921 gcggtcaaca gcaacgcgtg gcgttggcaa gggcattagt cttggaaccg gatgtcctcc
     4981 tgctggatga gccattgggt gcgctggatg cgaaaatccg ggtggatctc aggcgttcgc
     5041 tgaaaaacat ccagaagcag ttaggcgtgg ctgccatcct tgtgacacat gaccaggaag
     5101 aagcgtttga tctggccgac aggatcggtg tgatgagtta cggtcgattg gttgaagttg
     5161 gcacacctca ggaactgtac cagcgcccga agactgaatt cgttgcgtcc tttctgggga
     5221 cggcaaatat tctgctgggt caaaccgagg gagagcgcat ccaaattgga ccccatatct
     5281 tcaagctggc aaaggatgcc actcgcttta gcacagatgg gcgcgttcag gtagtgtttc
     5341 gccccgaaga tctagccctg gtccagaccg aagaggaact ggattgcaca ccgttgggtg
     5401 aaggcaccgt gacaagcctc ggcttcaatg gaccgacgga gcgaatcagg cttgaactac
     5461 cttctattcg cggcgttcgg gccattgcgc cttctgttcc ttttgggagt caaaacatac
     5521 tcatcgaagc cacgcgctcc ccagatcagg cttcagcgtt cccgctgggt gtgagtgaca
     5581 aagcctgggt ggggatacgt cgcatccatg ccctgtcaca tcccggactg aactttctgg
     5641 tcctgacaga tggatcgttg cgcagccaat cggccctcag cgttggaagt tacctggcgc
     5701 ggatggcaca tgcaagaatg acattgctcg gcgttgggaa agatgaaacc ctgctggata
     5761 cgtatctgca ggatgcccgc aagcaacttg gcagtggtat ggcctccatc gaagtgcgga
     5821 ccgatccgtt gccggcgccg atcgcgatca ccaaaattat tgagaaagat cccgtagacc
     5881 tggcgattgt cggctggagg ccggtggagg gaaccgaaat cgccgagcag attttacgac
     5941 atggagatca caacctgctg ctggccgctc atcccggtgc gcgaatagaa aaggcgttag
     6001 tctgtgttgc cagtggagaa cctggaaaag atgacgtaat gtttgcaggt cgcctgctgc
     6061 gccatctggg tgccagtgcg aaactgttca ccgtattaac accggcgatg aacaatgaac
     6121 atcagaaccg gctggctgtg cgcttcgtct ctggcggtgt acaaaccctg gaaatgttcg
     6181 gggtacccac cgaaagtgaa ttaaaagttg gggacccact gacagaaatt tcggaagaaa
     6241 taaagagagg cgcgttcgat ctggtggtca tgggctcgcc cttcccacag aaagatggtc
     6301 gcatcctgct ccatggcttg atcgaaggcg tgatgaaaaa cgccggtgat tgctccgtgc
     6361 tcatcgttcg atctcaccat cataaaaaca ccatcaaaca aaattggtca gaatctttat
     6421 acacatacat aaattaacaa aggacaaaaa atgtttacca aatttcgcaa gaccttactt
     6481 atttctcttt tgctgatttc cattctcgtc accggatgct cagcagggga gccaaccgaa
     6541 ggagccggaa ccgagcctgc caccggcgac gaagtcaaat taattctcgc tgcatacact
     6601 accccacgtg aagcgtaccg cgaactgatc ccgatctttc agcaacagtg gaaagataag
     6661 accgggcaga ccgttacctt tgaagaatcc tatcagggtt cgggtgctca gtcccgcgcg
     6721 gtcgctgaag gctttgaagc cgatatcgtc gcactttcac tggaagccga tgtgatccga
     6781 ctcgaagatg ccggtttgat cacgcaggat tggcgcagcc agccgttcgg cggaatcatc
     6841 agcacgtcgg tagtggcatt tgccgtgcgc gaaggcaacc cgaagaatat tcaggattgg
     6901 gccgatctgg cccaggctga tttggaaatc ctcacgccca acccaaaaac cagcggtggc
     6961 gcaatgtgga acgtgttagc actatatggt gcggctcaac gtggtcatgt ggtcggttat
     7021 tcggcggacg atgccggcgc tcaggatctc ctgctctctg ttttgcagaa tgtgaccgtc
     7081 atggacaaag ccgcccgtga ctccattgtg aatttcgaga agggcattgg cgatgtggcc
     7141 attacctacg aaaacgaagt ttttgtcggt cagcaggccg ggcagaccta cgagttggtg
     7201 cttcccacgt ccacgattca gattgataac ccaattgcca tcgtggatac ctatgcagat
     7261 aagcatggca cgcgcgaagt tgctcaagcc ttcattgact tcctatttac tccggaagcc
     7321 cagcaaatct ttgccaagca tggcttgcgc tccccagatc ccgaagtggc tgctgccacg
     7381 gctgagcaat atccgcctat cgaagacctg ttcacgatcg cagattttgg cggttggaag
     7441 gaagccactc ccaagtactt tggtgacaac ggcatcttct atcaggtctt tgctcaggta
     7501 caaggcattc aaatcagcga atagtaaatt aaccataaaa ggtgacagtc acttttagga
     7561 accaagtgac tgtcacctac tgcacgatgc gaaactttaa ataactcaca tgaacttatc
     7621 tacgacaatt tcttcaacta ataaaacgca acgctcgttt ccctggggac aattgggcat
     7681 ccgctcgatc gcgttggcct acctggcatt attgctcatc attccgcttc tggttatttt
     7741 gcaggacggt ctgaaggaag gaccggcgga actctggaga cagatcaccc tgccggcggc
     7801 gtggcacgcc ctgaagctca ccctttggac atccaccatc atgacggtca tcaacatcat
     7861 catgggcaca ctgaccgcat ttgtcctcgt gcgctacgag ttccccggca agaccatcct
     7921 caatgggatc attgatcttc cattggccat ccccacgctg gtaacgggcg tcatgcttgt
     7981 gatcctcttt ggtccgcagg aagcattggg aacatggtta aaaaatcagt ttgatatttc
     8041 gatcattttc gctccaccgg gaatcattct tgcactgtta ttcctcacct ttccctttgt
     8101 tgtgcgttcc gtacaacccg tgctattgga actggaccgt tctcaggaac atgcggcggc
     8161 tacactgggt gcgggaggct ggacgatctt ccgacgcatt attcttccgc ccttgaccct
     8221 gccagtcatt actggagcac tgctcagttt tgcacgtgcc atcggtgagt tcggttcaat
     8281 tgtcgtcgtg gccgggaaca tccccttccg ttcacagaca gctgctgtgt atgtgtttgg
     8341 tgaagtggaa tcagagaacc gactgggtgc cagcgccatg tccattgtga tggtgggaat
     8401 tgcgttcagt ttgatgatga tcgctgacta tctccgcaag cgttcgaaaa gtacaaagaa
     8461 ggaaaaccta tgagtattac cacctctgta cgtacttcgt tagcaaaaac tcagagcccg
     8521 gtgcgaagct ggggagagaa aaacaagggg aaatgggtgc agtggggact catcggaatt
     8581 gtgatcgcct atgtgagcat tctgatcctg gcgcccctca cagccttgac cctgggtgcc
     8641 ttcgagcaag gcttcggcgc gatcatcgag tcgttcaacc attcgttcgt gctcacgtca
     8701 ttttggaaca cgttgtggat cagcctggtc gtcgtgatca tacatgcggt cttcggaacc
     8761 atcgtggcct gggtgttcgt gcgccatcgc ttcccgggcc gtaatctgat caacgccctg
     8821 gtggatatgc cattcgccat ttcgccggtg gtggcgggct atatgttact gttgctcttc
     8881 ggacgcaacg ggctgctggc tccgctggtg gaggccacgg gcatacgggt tgcgtttgcg
     8941 ttcccaggaa tgctgctggc aacgctcttt gtcaccttcc catttat
//
LOCUS       DASTJP010000771        16117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_702223, whole genome shotgun sequence.
ACCESSION   DASTJP010000771 DASTJP010000000
VERSION     DASTJP010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16117
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702223"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1811
                     /locus_tag="VFQ23_22585"
     CDS             <1..1811
                     /locus_tag="VFQ23_22585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET9909451.1"
                     /translation="SILRPLFGPLFLLIISNAEGFLEVLHRKGFFWQFNQDGTATSAF
                     WTWLDMQELSEVPAQPLQWIPERFWWWWRASRVLQDYDLNGTFRETIDEFPFFSYLLG
                     DLHPHVLAMPFTLLAIAVALNLILGGWRGNINLFFGQLRINKTGFFVIALILGGLAFL
                     NTWDILVAAALIAFSYALARVREAGWSWERIEDILLLVIPVGLTAFVMYIPFYIGFDS
                     QAGGVTPNFMYPTRGAHLWVMWGTLFVPLFAFLIYLWRSGIPAQWRTGIFGMLGILFT
                     LLVAMFVIGILALRLKPELINPILQAQGLDVGAFISASMMRRLNYFGSLITLMALMIP
                     TFSFLAKANEDGEATENVAPSRPPSMIFVLLLMALGLLLILGPDFLYLRDNFGYRINS
                     VFKFYYQAWILLSLASAYGVSVLLVRFDNASQPQRSRVNFVFSLVLAMVLIVGLTYPA
                     LSIFNKTNNFKPPFGFTLDDFDRVQRENPDEAAAIQWLQSAPDGVVAEAVGNAYSNYA
                     RISIYTGLPTVLGWGNHEGQWREQSLQGSRQQDIETLYSTSDWATAQDIINRYGIRYI
                     YVGNLERNSYRISEDKFSRFLKPAFQQGSATIYEVP"
     gene            1834..3549
                     /locus_tag="VFQ23_22590"
     CDS             1834..3549
                     /locus_tag="VFQ23_22590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909452.1"
                     /translation="MNSSRLRYEGLLYCFAFLLALGFRFIQLGASSLTDSEANLALQA
                     LQIAQGKSPLLDPQPSYILFTSILFAVIESSNFIARFVPALVGSTLVFIPYLFREKLN
                     PRPALILAFLFAFDPGLVALSRQANGTILAVTFLLFAWGLWINRRVISAGIFAALALL
                     SGPSLWAGVLSLGLIWLFRRGTKNESDGDQSQATRWEEVRPVFLPLIVTLLLGGTLFF
                     VSPNGLSAWLLSLPEYLRGWVSPSAITPSRILLAFFAYEPLGIFIAALAIIRGFRTRS
                     DQLNRLSLWLGIALLLGIFYRQPAELVWVIIPLLTLAALELSRSLKVHAEERVEVGIV
                     VLAVFVLLVYIWFNVSNIAVNPYVQIPAVMPFFGQVQNPRYIIIAGASAILVVCLMMV
                     GFGWSPRTARLGAAWSFAIFFSLYSLAAAWGASGLRNPTGVELWSPDPKPLHERLLIS
                     SVNDVSEFSLGHIYSQPVTILGIDSPALEWALRNRTVEKVSVLDPQVAPPLVITPPMN
                     ELSLPSAYRGQDFTWRQTPVWDQIRNPDWWSWLVYRQLPRENETIILWARDDLFPDAR
                     ETVQP"
     gene            3601..4284
                     /gene="cmk"
                     /locus_tag="VFQ23_22595"
     CDS             3601..4284
                     /gene="cmk"
                     /locus_tag="VFQ23_22595"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET9909453.1"
                     /translation="MNSFKRPSIIAIDGPAASGKSTIGLRLAKALGYLFFDTGLMYRA
                     VTWLALDHDMNLHNEAAITSLAEKTPIDVAPASKSDGRACDVLVAGKDITWETRLPNV
                     ESNVSLVSAYRGVRAALTEQQRRIGKRGQIVMVGRDIGTVVLPDADLKIYLDASAEER
                     AQRRYDEIIARGREADYQDILEKVIERDRIDSTRDVAPLKAAEDASIVHTDKRSVDEV
                     FEQVLALCN"
     gene            4275..4976
                     /locus_tag="VFQ23_22600"
     CDS             4275..4976
                     /locus_tag="VFQ23_22600"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9909454.1"
                     /translation="MQLSDTNVYQVPIHFRLSRPILKTVFRGIFHLLANVKVAGKENV
                     PYGKPYIVAMNHISIFDPPFAGAFWPEQLEIIGASDVFDKPSQAWLLRGYGVIPVHRG
                     DYDRVLLTKIIRIIKSGSPLLIAPEGGRSHVPAMRRAKPGIAYLVEQTGVPVLPAGLI
                     GTTEDFWQRAKRGDRPSLEMRIGRPIFLPEITSKGTERHAARQKNADLVMSHLAGLLP
                     EEYRGVYAESAILPA"
     gene            4948..5673
                     /locus_tag="VFQ23_22605"
     CDS             4948..5673
                     /locus_tag="VFQ23_22605"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9909455.1"
                     /translation="MQNQQSSPPKPITDVWRPDLVALPRLTPARRAFRIFLRGVAKLV
                     TFFTMRTTIRGMENFPRHGPALIVINHLGDADAVLVGAAIPTTIDGMGKIELNDDRFV
                     GPIFRAYGVIWVHRGRPDRRALRAALDALAEGRIVGLAPEGRQSVTGGLEEGNEGAAF
                     LAMKSGAPILPLAMTGTENDHVYGHLKRWKRAPVTLTVGKPFQLREQPDRQKMLQEGT
                     RQIMQSLADLLPESYQGNYKPNS"
     gene            5724..8999
                     /locus_tag="VFQ23_22610"
     CDS             5724..8999
                     /locus_tag="VFQ23_22610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909456.1"
                     /translation="MQFLATLLTRLAYINPISLAGWMVWLGLAGVLVLALQNWRRYQK
                     EWNTRAWLIFGALFIATPLATLFLGLEFSASSALPVPGIPDEPPGSTMMIFSAVPWTL
                     AGGLLGPFAAAVLGMLSGIIRGIWDTHSLFSVLDLGLMGILFAVANRQRYRTFVYRLL
                     RQPLVSAVSLTLFHALLFLLSAFFTVSSTASVTERLDYALSNLGVASIAFAGEMLVAG
                     LVAQVIAMVFRAHWGALGMLQPSPTERSIETRFIFGTGTIVTILLITLLVGDWVIAGT
                     AARNLLRDRLKSSAELAAQNVPFFLETGQNLASQIANDSRLQDPNADLSAILSERIQS
                     IPYFNQIVVLDMQTRNILASYPSEPVFQITRPEEEGMLLIQQGIPNQIYTVPPSEDGT
                     GAGTSFLAVIPQMGRVVIGRTYLSANPYTRSLVSNLNSLVEVNGAGLLVADGMIVYHS
                     EATQTWTAYQGQLSESPAFFDETASLGTRQLVYYQPVEGYPWAVVLTVPAQATQQLAI
                     NIALPISLMIILLGIIALITMRANLRAVTRSLQSLATEAGHIATGRLDRPLNTEGVDE
                     LGELRRAFEQMRVSLQARLQDLNRLLVASQGVASSLTIGDALRPVLEAVVDNGASSAR
                     VVLVREMLPTTVETPLRFADGVEQDVYMHLDQQILALTEQQERLVMATLSRTRGLMMD
                     PNLPHPESLIAIALRHENRFYGVLWAAYNKQRIFSEADLRFISTLASQAALAVTNIRL
                     FLTVEVSRRQLEAILNSTPDPVLVTDASNRMILANPAATHVFGVTIRRGEKPELERTI
                     QVKPLNELLLSSSTERRSAEIRMPDGKTYLAMASAMTVDGRTIGRVCILRDVTQLKEI
                     DTLKSDFVATVSHDLRSPLTLMRGYATMLEMAGTLNEQQKGYARMIVQGVDNMAKLVN
                     NLLDLGRIDFGVGLQVESIPVLDILERVTGSLQMHAKQKNISLGVEIPKDMPHAIEAD
                     QSLLHQAIYNLVENALKYTPEGGEVTIELQTSPSSLTFAVQDSGIGIPKSDVPRLFEK
                     FYRGTNREALAQRGTGLGLAIVKSIAERHGGKVWVESELGKGSTFHLQVPLEQPEESA
                     PVP"
     gene            complement(9056..11020)
                     /locus_tag="VFQ23_22615"
     CDS             complement(9056..11020)
                     /locus_tag="VFQ23_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9909457.1"
                     /translation="MLTSENTQPEILRRQSDYKPPRSVSHWFIGRPLSTADAPHQTIG
                     KRIGLAVFASDALSSTAYATQEIMIILAVAGTMAFGYVFPISLAIVALMTIVTISYEQ
                     TIHAYPGGGGAYIVARDNLGETPAQIAGAALLTDYILTVAVSISAGVAQITSAYPDLF
                     PYRVWISVGAVFFIMLINLRGVKESGTAFSIPTYFFIVMLFVTVGTGMIRHFTGSLGV
                     VIDPPHLEVAGIVSTITPFLILHAFSSGTAALTGIEAISNGITAFKEPRSRNAGITLI
                     WMAVILATLFLSISFLASHIGAIPSESETVISQLGRTIFEGRGTLYLLVISATTVILI
                     MAANTAFADFPRLSALAAQDGFLPRQLTFRGSRLVYSNGIITLSIIASILIIIFKASV
                     TLLIPLYAIGVFLSFTLSQTGMARRWWKIGHLKKDQEIIEQGSTLKYEPGWKYKMLIN
                     GFGGFCTAIVMVIFAVTKFREGAWIVLIIIPLLVKMFFTIHHHYNDLASHLSLDKFSG
                     LPARQMRHRVLLPVSGIHQGALEALRYAKLLSDDVTAIHISTDPTETERVQKKWKIWG
                     EGTRLVILDSPFRLLIEPLVEYVEDIVKNSQPNETITIVVPQLMPSKRWHQALHMRTA
                     DVLRQELLSTHGVVVTDVPYHVYKEAEESE"
     gene            complement(11061..11702)
                     /locus_tag="VFQ23_22620"
     CDS             complement(11061..11702)
                     /locus_tag="VFQ23_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909458.1"
                     /translation="MNKSITQIQTIPGLISPVSELGIESIRRLIVLVPDAELDYAAAT
                     RRVWELAGELKAHILFIGLCKDPEQEPGLRRQLITMTAMVQDGNASAETCIETGTNWV
                     TIVKRNYRPGDLVVCFAEQRAGFLQKQLSQILQSSLHIPVYIISEAFMEKPGMNWRSQ
                     ITLWTGYAGIVIAFCLLQVRIVQLPKDGFQSLLLLLSIIPEFWLIWVWNNLFS"
     gene            complement(11866..12561)
                     /locus_tag="VFQ23_22625"
     CDS             complement(11866..12561)
                     /locus_tag="VFQ23_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909459.1"
                     /translation="MNTEPHILVIDDELQILRALRTILTEKQFKVTTASRGEEGLTLA
                     AAQEPDLIILDLGLPDMDGVEVCTRLREWTQCPIIVLSVRDSERDKVAALDKGADDYL
                     TKPFGIEELLARVRVALRHSARVHGIQEKVVRAGPLTIDLAWHIVKFGEEEVKLTGTE
                     HKLLAYLAANHGRVLTHQTILTNVWGPADADHTEYLRVYMRQLRKKLEADPERPQYIL
                     TEPGIGYRFIADE"
     gene            complement(12558..14024)
                     /locus_tag="VFQ23_22630"
     CDS             complement(12558..14024)
                     /locus_tag="VFQ23_22630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909460.1"
                     /translation="MNSANMQSSSFLFRTQRYLLPTVFIALVTSVFFTLRDVLDTTLI
                     ALLYLIPLGMITAYWGLGPGITSALLTFLVFNYFFIPPYYLLTVHQPSDVVILIVFLI
                     VAVVISQLVGRAQAGQAIATAREREATQLYELSVALAGLHSDRAIAEILAKRIRPIAQ
                     GEVELNIMGAQPFVFHLNENHPPTRPPDFVVPIQAARGILGEIRIWRTPPAVSSGERR
                     LLQTFASQGALALERAWLAQAESRAQVLEESDKLKSAILSSVSHELRTPLSTIKAAAS
                     SLRGGEVSWDSPARAELIEAIDDEADHLNMLVGNLLDMSRIDSGALKPKREWNILSEI
                     VGSVLARMKRLTDTHRIEVDIPETLPLVPVDYVQMEQVFTNLLSNSLKYAPGNTTINI
                     RAAKENEMVHVQVSNQGPHVPIEHLERIFDKFYRTTDADRIAGTGLGLSICKGIIEAH
                     QGHIWAENLTNGIAFNFTLPLIWDGVLPSQLPFDAEDE"
     gene            14283..>16117
                     /locus_tag="VFQ23_22635"
     CDS             14283..>16117
                     /locus_tag="VFQ23_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9909461.1"
                     /translation="MIDPEDNQDTSLIHRTTTEEPSRSFRHFLIGRPISTAKAEHETI
                     GKAVGLAVFASDALSSTAYATQEMLVILAGAGTIAFGYVFPISIVIVALLIIVVVSYQ
                     QIINAYPGGGGAYIVAADNLGQFPALVAASVLLMDYVLGVSVSISSGVAQIVSAFPGL
                     YDYRVPLCIVFVALITIVNLRGVRESGLAFAIPSYFFIIIMFITIGTGLFRMVTGTLG
                     NVINPPEIEHVGEALTGISLFLILHAFSSGTTALTGVEAISNGITAFKEPRSRNGNIT
                     LTWLALILSLLFLGISFLTREIQAVPSEKETVISQMARTVFGGQGLFYLLAIAGTTFI
                     LILAANTSFAGFPRLSAKLASDGFMPRQLTYRGTRLVYSYGIISLGAAASILIVIFQA
                     SVTRLIPLYAIGVFLCFTLAQVGMARRWRRSGQLGNDESTSKSKQSIRYDSKWMLKMF
                     ANGFGAICTAVVMIVFIITKFVDGAWIILAVTPIFISVLLWVHRHYKSMAQGLSLENY
                     GEPPPYNLRHRVIVPISNVHQGTLAALRYARALSDDITAVHVSIEPEDTEKTVKKWET
                     WGRGTRLVIVDSPYRLFLEPLLTYIEDIIASRQANETITIVVPHFV"
BASE COUNT         3791 a         3982 c         4090 g         4254 t
ORIGIN      
        1 catcgatcct acgtcctctc tttggccctc tgtttttgct tatcatttca aacgcagaag
       61 gctttctcga agtcctgcat cgaaagggat tcttctggca attcaaccag gatgggacag
      121 ccacgtctgc attctggacc tggcttgata tgcaggaatt gagtgaggta cctgcacagc
      181 ctctccaatg gattcctgaa cgcttctggt ggtggtggcg cgcctcacgc gtcttacagg
      241 actatgactt gaacggaacc ttccgcgaaa ctatagatga gttcccattc ttttcgtatc
      301 tgctggggga tttgcatccg catgtgttag cgatgccctt tacattgttg gcgattgctg
      361 tggctttgaa tctcatcctt ggtggctggc gcgggaacat taatctcttt tttggtcaat
      421 tacgcatcaa caaaacaggc ttctttgtta ttgcgctgat attaggcggg ctggcctttc
      481 tcaacacgtg ggatattctc gttgccgcgg cgttgatcgc tttcagctac gcgttggcac
      541 gtgtccgtga ggcaggctgg agctgggaaa ggatcgaaga catcctactg ctggtgattc
      601 cagtagggct cacagccttt gtaatgtata ttccgttcta tattggtttt gattcacagg
      661 caggcggcgt tacaccaaac ttcatgtacc caacgcgcgg tgcgcatctg tgggtcatgt
      721 ggggcacgct tttcgttcca ctttttgcat tccttatcta tctttggaga agcggcatcc
      781 cagctcaatg gcgaacaggg atttttggaa tgctgggtat tctctttacg ctcctggtag
      841 cgatgtttgt gatcggtatc ctggcattga gattgaagcc tgaacttatc aatccgattt
      901 tgcaggcaca ggggcttgat gtaggcgcgt tcatttcagc tagcatgatg cgccgattga
      961 attattttgg cagcctgatt acgctgatgg cgttgatgat tcctactttt tcgtttctgg
     1021 caaaagcaaa cgaggatgga gaagcaacgg agaatgttgc gcccagtcgt ccaccctcga
     1081 tgatctttgt ccttttattg atggcgcttg gcttactgct tattcttggc ccggactttc
     1141 tttatcttcg tgacaatttt ggatatcgta ttaactcggt tttcaaattt tattatcagg
     1201 cgtggatatt attgagtctc gcttctgcct acggagtttc tgtcctgctc gttcgcttcg
     1261 acaatgcatc tcaaccacaa cgcagtcgtg taaactttgt ttttagtctc gtgcttgcta
     1321 tggttctgat cgttggtctc acctatcccg cgctcagcat cttcaacaag accaataact
     1381 ttaagccgcc ctttggattc acgctggatg attttgatcg ggtacaacgc gagaatcccg
     1441 atgaagcggc cgccattcaa tggcttcaat ctgcaccaga tggcgtggtc gctgaggctg
     1501 tagggaacgc atactccaac tatgcgcgca tctctatata cacaggcttg ccaacggtcc
     1561 tggggtgggg aaatcatgaa ggtcagtggc gtgaacaatc tctacaaggg tcgcgccagc
     1621 aggatatcga aacgttgtat tccacttcag attgggcgac agcccaggac attattaatc
     1681 gttatggtat tcgatacatt tatgttggta atctggaacg gaattcctac cgcatcagtg
     1741 aagacaaatt tagtcgcttt cttaaacccg cctttcagca gggtagcgcg acgatttacg
     1801 aagtacctta atatcgtagg gtaaaatact tttatgaatt cttctcgact cagatatgaa
     1861 ggcttgctat attgtttcgc ctttctccta gccctgggtt tccggtttat ccaacttggg
     1921 gctagttctt taaccgactc tgaagcaaac ctggctctgc aagccttgca gattgcacag
     1981 ggtaagagtc ctctgcttga ccctcaacct tcctacattc tttttaccag tattttattt
     2041 gctgtcattg aaagctcgaa cttcatcgcc cgtttcgttc ctgccctggt tgggagtacg
     2101 ctcgtcttca tcccttattt gtttcgtgaa aaactaaatc cgcgtccggc gctgatcctg
     2161 gcattcctgt ttgcatttga ccccgggctt gttgcgctgt cgcgtcaggc aaatgggacc
     2221 attcttgcgg tgacgttcct tctctttgca tggggtctgt ggatcaatcg acgggtcatc
     2281 tcggctggca ttttcgctgc actggcgctt ctctccggac cttcactttg ggcaggagtc
     2341 ctttcattag gtttgatctg gcttttccgc cgtggaacga aaaacgagtc agacggtgat
     2401 caatcgcagg caacgcggtg ggaagaagtc cgaccggtct tcttaccgct gattgtcact
     2461 ttgctcttag gaggtacgct tttcttcgtc tcccccaacg gacttagcgc gtggcttttg
     2521 tctttaccag aatatttgcg aggctgggtc tcaccttccg ccataacccc gagccgaatt
     2581 ctgctggctt tcttcgccta tgaaccattg gggattttta tcgccgccct tgcaatcatc
     2641 cgcgggtttc gcacaagaag cgaccaactt aaccgcttga gtctttggct gggcatcgca
     2701 ttgctgctgg gaattttcta tcgacagcct gctgaactcg tctgggtcat catccctttg
     2761 cttacgcttg ctgccctgga actctcacgc agtttgaagg tccacgccga agagcgtgtg
     2821 gaggtgggga ttgtcgtcct ggcagtcttc gtcctgctgg tgtatatctg gttcaacgtt
     2881 tcgaacattg ctgttaatcc gtatgtacag attccggctg tcatgccttt ctttggtcag
     2941 gttcaaaatc ctcgctacat aatcatcgct ggcgcttccg ctattctcgt tgtgtgccta
     3001 atgatggtcg ggtttggctg gtccccgcgc acagcgaggc ttggtgcagc ctggtccttc
     3061 gccatctttt tcagccttta cagtcttgca gcggcctggg gcgcaagcgg actacgaaac
     3121 ccaactggtg tggaactatg gtcacctgac ccgaagcctc tccacgaacg tttactcata
     3181 tccagtgtca atgatgtttc cgaattcagc ctgggtcaca tctactccca gcctgtcacc
     3241 attcttggaa ttgattcccc tgccctggaa tgggcactgc gaaatcgtac tgtcgaaaaa
     3301 gtttcggtgc ttgacccgca agtcgcgccg ccgcttgtca tcacaccgcc catgaacgag
     3361 ctgagtctcc catctgctta tcgtggacaa gatttcacct ggaggcaaac tccagtttgg
     3421 gatcaaattc gaaacccgga ctggtggagc tggcttgtct atcgtcaact gccaagagag
     3481 aacgaaacga tcattttatg ggcccgcgat gatttattcc cagacgcgcg cgagactgta
     3541 caaccatgag cctggagcca ggtagtttgt gttgccttga cagagtggga tagtaaagaa
     3601 atgaattcct tcaaacgacc atccattatc gcgattgatg gacccgcagc ctccggaaag
     3661 tcgacaatcg gtttgcgact cgcaaaagct cttggctatc ttttctttga tacggggttg
     3721 atgtatcgtg ctgtcacctg gctcgcgctt gaccatgata tgaatctgca taatgaagcc
     3781 gcgatcacgt cactggctga gaaaacgcct atcgatgttg ctcccgcttc aaagtctgat
     3841 ggacgcgctt gtgacgtcct ggtggcaggg aaagacatca cctgggaaac acgtttgcca
     3901 aatgtagaat cgaatgtttc tttggtctct gcttatcgcg gggttcgcgc ggcgttgacg
     3961 gaacagcaaa ggcgtatagg aaaacgcggt caaattgtaa tggtagggcg cgatattggc
     4021 acggtggtgt tgcctgacgc ggatcttaaa atttatctcg acgcaagtgc tgaagaacgc
     4081 gcccagaggc gttatgatga aatcatcgcg cgcggcaggg aagcagatta tcaagatatt
     4141 ctcgaaaaag tgatcgaacg cgaccggatc gattcgactc gcgacgtagc gccgctgaaa
     4201 gctgcggaag atgcctcgat agttcatact gataaacgta gtgtggacga ggtctttgaa
     4261 caggtactgg ctttatgcaa ttgagtgaca ccaacgtcta tcaggtgccg attcattttc
     4321 ggctcagtcg tcccatctta aagactgttt ttcgtgggat ttttcatcta ctggcaaatg
     4381 tgaaggtggc gggtaaagaa aacgtaccgt atggcaagcc ttatattgtg gcaatgaatc
     4441 atatttcaat ttttgatcct ccatttgcag gcgcgttctg gccagagcaa ctggagatca
     4501 tcggtgcctc cgatgtgttc gataaaccca gccaggcatg gttgttaaga ggctatggtg
     4561 tgatccctgt ccatcgcggt gattatgatc gcgtgttgct aacaaaaatc attcgcatca
     4621 ttaagtctgg ttccccgctt ttgattgcac cggaaggtgg ccgttcgcat gtgcctgcca
     4681 tgcgtcgggc aaagccaggt attgcttatc ttgttgaaca gacaggtgtg cctgtcttac
     4741 ccgcagggct gatcggcaca acagaagact tctggcaacg cgccaaacgc ggcgacagac
     4801 cttcactcga aatgcggatc ggtcggccca ttttccttcc ggagatcacg tccaagggca
     4861 cggaaagaca tgcagcgcgc cagaaaaacg ccgacctggt catgtcgcat cttgcaggat
     4921 tactaccaga agaatatcgc ggggtctatg cagaatcagc aatcctcccc gcctaaacca
     4981 atcacagatg tgtggcggcc agacctggtc gccttaccgc gtctcactcc agcacgccgc
     5041 gcgtttcgta ttttcttgcg cggggtggca aaacttgtca ccttcttcac catgcgcacc
     5101 accatccgtg gcatggaaaa cttccccagg cacggaccgg ccttaattgt gatcaatcac
     5161 cttggcgatg cggatgcagt tctagtaggt gctgccatcc caaccaccat tgacggaatg
     5221 gggaaaattg aattgaatga tgatcggttt gttggaccca tcttccgagc ctatggggtg
     5281 atctgggtac atcgcggacg acctgatcgc cgtgccttac gcgctgcatt agacgctttg
     5341 gctgaggggc gtattgtggg gcttgcacca gaaggaagac aatctgtcac cggcgggctg
     5401 gaggaaggga atgaaggcgc tgcgttcctg gcgatgaaat cgggtgcgcc aatccttcct
     5461 cttgccatga ctgggacaga aaatgaccat gtttacggcc atcttaagcg atggaaacgc
     5521 gcgccagtca cattgacagt gggaaaacct ttccaattac gggaacagcc tgatcgtcag
     5581 aaaatgcttc aagaaggcac gcgccagatc atgcaatcgc tggcagacct gcttccagaa
     5641 tcgtaccaag gcaactacaa acccaattca taacaagcgt gagacggcgc tctgcgcatg
     5701 aattaacgct ataatcattg cctatgcagt tcctggcaac gctgttgaca cgtcttgcct
     5761 atatcaaccc catatctctg gcagggtgga tggtctggct tgggttggcg ggcgtgttag
     5821 ttcttgccct tcaaaactgg cgcaggtatc aaaaggaatg gaacacgcgc gcatggttga
     5881 tctttggcgc gctgtttatt gcgacacccc ttgcaacctt atttttaggg ctggagtttt
     5941 cagctagctc tgctttgcct gtgcctggca taccagatga accgccaggc tccaccatga
     6001 tgatcttctc cgccgtccca tggacattgg caggtggctt gctgggtccg tttgcggcgg
     6061 cggtgttggg tatgttaagc gggatcatac gcggcatctg ggacacacac agtcttttct
     6121 cggtgcttga tctgggtttg atggggatac tattcgcagt ggcaaatcga cagcgatatc
     6181 gcacttttgt gtatcgctta ttacggcaac ctctggtaag tgcggtcagc ctgacattat
     6241 tccatgcgtt actctttttg ttgagtgcgt tctttacggt ttcttcaaca gcttctgtta
     6301 cagaacgcct ggattatgcg ctgagcaacc ttggtgtggc aagtattgca tttgccggag
     6361 aaatgttggt tgcagggctg gttgctcagg tgatcgcgat ggtctttcga gcccactggg
     6421 gcgcgttagg catgctacaa ccctctccta cagagaggag catcgagaca cgtttcatct
     6481 ttggcacagg cacaattgtc acaatccttc tgattacact gctggtcggt gactgggtta
     6541 tcgctggcac agccgcgcgt aatttattga gagatcgcct caagagttca gctgaactgg
     6601 ctgcacagaa cgtgcccttt tttctggaaa cgggccaaaa cctggcttca cagatcgcga
     6661 atgattctcg cctgcaggat ccgaatgctg atctgtctgc cattctcagc gagcgcattc
     6721 aatccatccc gtacttcaat caaattgttg tgctcgatat gcagacaaga aatattcttg
     6781 cgagctaccc ttctgaaccg gtttttcaaa ttacgagacc tgaagaagaa ggtatgctac
     6841 tcattcagca aggtattcca aatcaaattt ataccgttcc gccatctgaa gatgggactg
     6901 gggcgggcac gtcctttctc gcggttattc ctcaaatggg acgcgtggtc atcgggcgta
     6961 cctatctaag tgcaaatcca tacactcgtt cattggtcag caatctgaac agcctcgtcg
     7021 aagtcaatgg agcaggcttg ttagttgcag acgggatgat tgtctatcac tctgaagcga
     7081 ctcaaacatg gacggcttat cagggccaac ttagtgagtc cccggccttc tttgacgaga
     7141 ccgcctcgct tggaacgcgt cagttagtct actatcaacc tgtagagggt tatccctggg
     7201 cagtcgtgct gacagtcccg gcgcaggcga cacagcagct cgcaatcaac attgcattgc
     7261 cgatctcact gatgattatt ttgctgggca tcatcgcctt gattaccatg cgtgcgaacc
     7321 tgcgagcagt cacgcgttct ctacaaagcc tggcgacaga agccggacac atcgcgacgg
     7381 gcagattgga tcgtccgctc aataccgaag gggtggatga actcggcgaa ttgcgacgcg
     7441 ccttcgagca aatgcgcgtc agcttacagg cgcgtcttca ggacctcaac cgcttgctcg
     7501 ttgccagcca gggagtggcc tccagcctga cgattgggga tgcactgcgc cctgtgcttg
     7561 aagccgtggt ggataacggc gcgagttccg cccgcgttgt gctggtgcgc gaaatgcttc
     7621 cgaccacggt cgaaacgccg ctccgctttg cggatggggt cgagcaggat gtttatatgc
     7681 atctggatca acaaatcctg gcgctcacag aacaacaaga gcgactggtc atggcaactc
     7741 tctcccggac ccgcggcttg atgatggacc caaacctgcc tcatcctgag tccttgatcg
     7801 cgattgcctt acgtcatgag aatcgttttt atggtgtttt gtgggcagcc tataacaaac
     7861 agcgcatctt ttctgaggcc gacctccgtt ttatcagtac actggcaagc caggctgcgc
     7921 tggcggtcac aaacatccgt ttgttcttaa cggtggaagt gagtcggcgt cagttggaag
     7981 ctattcttaa ctctacgcca gatccggtcc tggttacaga tgcatcaaat cgtatgatcc
     8041 tggcaaaccc agctgcaact cacgtttttg gcgtaacgat ccgtagagga gaaaagccgg
     8101 aacttgagcg aaccatacag gtcaagccgc tgaacgaact gcttttatcc tcctcgacag
     8161 agcggcggtc ggcagagatc cgcatgccag acgggaaaac ctatcttgcg atggcttcgg
     8221 caatgactgt ggatggaagg acgattggtc gtgtttgtat tttgcgcgat gtgactcaac
     8281 tcaaggaaat tgacaccttg aagtctgatt tcgttgcgac tgtcagccat gatttgcgct
     8341 cgcctctcac cctcatgcgt ggatatgcaa ccatgcttga aatggctggc acattgaatg
     8401 agcaacaaaa aggctatgcc cggatgatcg tgcagggcgt ggacaacatg gcgaagctgg
     8461 tcaataatct gctggatttg ggacggattg attttggcgt tggcctgcaa gtggaaagta
     8521 tcccggtgct ggatatcctc gagcgtgtga ccggcagcct gcaaatgcat gcgaagcaaa
     8581 aaaatatctc actgggtgta gagatcccca aagatatgcc gcatgccatc gaagccgatc
     8641 aatccctatt gcatcaggct atttacaacc tggtggaaaa tgcactcaaa tatacgcctg
     8701 aaggcggcga ggtcacgatt gagctgcaaa cctcgccttc gtcattgact tttgcagtgc
     8761 aggatagtgg tattgggatt ccaaaaagcg acgtcccgcg cttgtttgaa aaattctatc
     8821 ggggtaccaa tcgagaggcg ttagcgcagc gtggcaccgg tcttggactt gcgattgtca
     8881 agtcgattgc cgaacggcac ggtggcaaag tctgggtgga aagtgagctt ggtaaaggca
     8941 gtacgttcca tctgcaggtt ccgttagagc agccagagga atcagcgccc gttccataac
     9001 caggctttgc ccagattctt tacgagtctt ttccgtcatc gaacgacagt acagattatt
     9061 cactctcttc cgcttctttg tacacatgat acggaacatc ggtgacaacg acaccgtgcg
     9121 tggataatag ctcctggcgc aatacatctg cggtacgcat gtgaagtgcc tgatgccagc
     9181 gtttggatgg catgagttga ggaacaacaa tcgtaatggt ctcatttggc tggctatttt
     9241 taacgatatc ctcaacatat tccaccaaag gttcgatcag taaacggaat ggtgaatcga
     9301 gaatgaccag gcgtgtgcct tctccccata ttttccattt tttctgaacc cgttctgttt
     9361 ctgtcgggtc agtcgaaata tgaattgctg ttacatcgtc tgacagtagt tttgcgtaac
     9421 gcaacgcctc taatgcgccc tggtgaattc cgctcacggg cagtaggaca cgatgccgca
     9481 tctgacgtgc aggcaatcca ctaaacttat ccaacgaaag gtgcgatgcc aggtcgttgt
     9541 aatgatggtg aatggtaaag aacattttga ccagcagggg aatgataatg agcacgatcc
     9601 aggcgccttc acgaaatttg gtgaccgcaa atatgaccat aacaatcgct gtgcaaaagc
     9661 caccaaaacc attaatcagc attttgtatt tccagcccgg ctcatatttg agtgtggagc
     9721 cttgctcgat aatttcctgg tctttcttca agtgaccgat cttccaccag cggcgtgcca
     9781 tgcccgtctg tgaaagggta aaggatagga acacgccgat ggcatacaga ggaatcaaca
     9841 aagtcacact ggctttgaag ataatgatca ggatggatgc gatgattgaa agcgtgataa
     9901 ttccgtttga atagactaga cgactgccgc gaaaggtcaa ttgacgaggc aggaagccat
     9961 cttgtgcagc gagtgcactc aagcgcggaa agtcagcaaa ggcggtattc gcggccataa
    10021 taagaataac ggtcgtagct gaaatgacaa gtaaatatag agtaccgcgt ccttcaaaaa
    10081 tcgtacgccc caactgggag attacggttt cagattcaga tggaatcgcg ccaatgtgac
    10141 ttgccagaaa ggagatgctc aaaaataggg tggcaaggat aaccgccatc cagatgagag
    10201 taatacctgc attacgactg cgaggttctt taaaggcagt gattccattc gatatggctt
    10261 caattccggt cagggcggct gtaccacttg agaatgcatg gagaatgagg aaaggggtga
    10321 ttgttgaaac gatacccgca acttcaaggt gaggcgggtc aataacaacg cccaagcttc
    10381 cagtgaagtg tcgtatcatg ccggttccaa cggttacaaa tagcattacg atgaaaaaat
    10441 aggttgggat ggaaaacgca gtgcccgatt cttttacgcc gcgcagattg atcagcataa
    10501 taaagaacac agcacccaca gaaatccata cacgataagg gaagagatcc ggataggctg
    10561 aggtaatttg cgcaacaccc gctgaaatag atacagccac tgtaaggata tagtccgtaa
    10621 gcaaagccgc accagcgatc tgcgcaggcg tttcaccgag attatcccgg gcgacaatgt
    10681 atgcgccacc gccgccggga taagcatgga tggtctgctc gtatgaaatg gtaacaatcg
    10741 tcattaatgc gacaattgcc aacgagatgg gaaaaacata cccgaatgcc atcgttccgg
    10801 caaccgcaag aatgatcata atttcctgcg tagcatacgc tgtggaagat aaagcatcgg
    10861 aggcaaagac agccagccca attcgtttgc caatggtctg atgtggcgca tcggcagtgg
    10921 agaggggccg accaataaac cagtgactca cggaacgtgg cggtttgtaa tcactttgcc
    10981 gccttaggat ttcgggttga gtattttcgg aagttaacat ttctacgttc aattctccag
    11041 gatataaggt cgataaaata tcagctaaat aagttgttcc acacccagat cagccagaat
    11101 tcagggatga ttgaaagcag gagcagcagg ctttgaaatc catcttttgg tagttggaca
    11161 attctgactt gcaacaaaca aaatgcgatc acaattccag cgtaccctgt ccacaaagta
    11221 atttgtgaac gccaattcat cccgggtttt tccataaaag cttcggaaat aatgtataca
    11281 ggaatatgca ggcttgattg caggatttgg cttaactgtt tttggaggaa cccagcgcgt
    11341 tgctctgcga agcaaacgac caggtctccc ggacggtagt tccgctttac gatcgtcacc
    11401 cagtttgtgc cggtttcaat acaggtttct gcagaagcgt ttccatcctg caccatggca
    11461 gtcatcgtga tcaattgtct acgaagacct ggttcctgct ccggatcttt acatagccca
    11521 ataaatagga tgtgagcttt taattcaccg gccaattccc atactcggcg agttgcggct
    11581 gcatagtcca attctgcatc gggaacaagc acaattaacc ggcggatcga ttcaatgcca
    11641 agctcggaaa ctggagaaat taagcctggg atagtctgta tttgcgtaat agatttattc
    11701 atcgttacct ctttgtcatc catgatctta accagcctgc ataaaacggt gacaaaaaaa
    11761 ttgaccaatc gtataaagga caaataaaaa acgccctttg cgagggcgtt ataaggtggt
    11821 tatcatagtt ttggatgccg aaaatagcgg ttttagcctc tggttttatt catccgcaat
    11881 aaaacgatat ccaatgcctg gctcagtgag aatgtattgt gggcgttccg ggtcagcttc
    11941 aagcttcttg cgcagctgcc gcatgtaaac tcgcaggtac tcggtatgat cagcatcggc
    12001 gggtccccaa acgttcgtca gaatggtttg atgggtgagg acccgtccgt gattggccgc
    12061 cagatacgct aatagtttgt gttctgtgcc cgttaacttg acttcctctt caccgaactt
    12121 cacaatgtgc caggccagat caattgtaag tgggcctgct ctgacgactt tttcctgtat
    12181 cccatgaacc cgcgcggaat gccgcaatgc gacacgtaca cgcgctagga gctcctcaat
    12241 gccaaacggt ttcgtaagat aatcatcggc tcccttatcc aatgcagcca ctttgtctcg
    12301 ttcgctatct cgaaccgaga gaacaatgat ggggcattgc gtccattcgc gcaggcgtgt
    12361 acatacttcg acgccatcca tatccgggag accgaggtcc aggatgatga gatcaggctc
    12421 ctgggcggca gcgagtgtga gtccttcctc acctcgactg gccgttgtca ccttgaactg
    12481 tttttcggta agaatggtcc gtaaggctcg caggatttgt agctcgtcat caatgacgag
    12541 aatatgtggt tcggtattca ttcatcctct gcgtcaaatg gaagttggga aggcaagact
    12601 ccgtcccaga tcagcggcaa agtgaaattg aaagcgatcc cgttcgtcaa attctcagcc
    12661 catatgtggc cctgatgtgc ttcaatgatc cctttgcaaa tcgaaagtcc aagccctgtg
    12721 ccagcgatgc ggtcagcatc cgtcgtccgg taaaacttat cgaaaatgcg ttcaaggtgt
    12781 tcaataggca cgtgtggtcc ttgattgctg acctgaacgt gaaccatttc attctcctta
    12841 gccgcgcgaa tattaatcgt cgtatttcct ggggcatact ttaaactgtt actgagcagg
    12901 ttggtaaaca cctgctccat ttgtacataa tcgacaggca caagtggaag ggtctctgga
    12961 atgtcaactt cgattcgatg cgtatccgtc aaacgtttca tacgagccag cacactgcca
    13021 acgatctcag acaaaatatt ccattccctc tttggcttaa gggcacccga atcaatccgc
    13081 gacatatcca gtaaattacc aaccagcata tttagatgat cggcttcatc gtcaatcgcc
    13141 tcgatcaact cagctcgagc gggtgaatcc caactcacct ctccgccgcg taagctggac
    13201 gcagctgcct ttatggtgga caaaggagtt ctcaattcat gagaaacgga agacaaaatg
    13261 gcagatttca atttgtcact ttcttcgagc acctgggcgc gtgattctgc ttgagccagc
    13321 cacgcacgtt caagggccaa tgctccctga cttgcaaagg tctgtaaaag ccgcctctcg
    13381 ccagaggaga cggctggcgg cgtcctccaa attcggatct ccccaaggat cccgcgtgcg
    13441 gcctgtatcg gaacgacaaa gtctggcggg cgcgtcggtg gatggttttc atttaggtga
    13501 aatacgaagg gctgagcacc cataatattg agttcaactt caccttgcgc tatgggccga
    13561 attcgtttcg caaggatttc cgcgatagcg cggtcgctgt gcagacctgc aagtgctaca
    13621 ctcagttcgt aaagctgggt ggcttcacgc tcccgtgctg tagcgattgc ctgaccagcc
    13681 tgcgcacgcc ccacaagctg actgatcacc accgcgacga tcagaaagac aatgagaatc
    13741 acaacgtccg agggctggtg aactgtcagg aggtaatacg gtggaatgaa aaaataattg
    13801 aacacgagaa acgttaacaa tgcactcgtg atgccaggtc caagccccca ataggcagta
    13861 atcatcccta gtggaatcaa atacaaaagg gcgatcagag tcgtatccag cacatcgcga
    13921 agagtgaaga atacagaagt aaccagtgcg atgaatacag tggggagaag atatcgctga
    13981 gtccggaata gaaaagatga actctgcatg tttgcagagt tcattatacc ttttcggttg
    14041 tatcgaaatt gcttgatctt tttgctggga gtggataatt tcgtgggctt agtcaccgag
    14101 acctgagaga attttctcaa agaactcagt gttctccccc ggcactgccc gccagggtaa
    14161 gtgtgtggcg aatccgactc tagtccatcc ttttagtcac tccctttcct ggcttgttaa
    14221 caatctaaaa ttgatttcgt ttataatgca ttatcctcca tgaggacttg cgccgaattt
    14281 acatgattga ccctgaagac aatcaagata cttcccttat acatcgcaca accaccgaag
    14341 agccatctcg tagtttccgc catttcctaa tcggtcgtcc tatctccact gcaaaagccg
    14401 aacatgaaac catcggcaag gcagtggggc tggctgtgtt cgcctccgat gcactttcct
    14461 ccactgccta tgcaacacag gaaatgctgg tcatattggc tggtgccggg acaatagcat
    14521 ttggctatgt gttccccatt tccattgtta ttgtcgcact gctcatcatt gttgtagttt
    14581 cgtaccagca gatcatcaat gcttatccgg gtggcggtgg cgcctatatc gtagcagctg
    14641 ataatcttgg tcaatttcct gcgttggtgg ccgcatcggt tctcctgatg gattatgtgt
    14701 tgggtgtgtc tgtatcaatt tcctcaggtg tcgctcagat cgtatctgca ttccccgggt
    14761 tgtatgatta tcgtgtgcca ctttgcatcg tatttgtggc gctaattacc atcgtcaatt
    14821 tgcgcggggt ccgggaatca ggtttggcct ttgccatccc cagttacttt ttcatcatta
    14881 tcatgttcat tacgatcgga acaggattgt ttcgaatggt caccggaacg ctgggaaatg
    14941 taattaaccc gccagaaatt gaacatgtag gtgaggctct aacaggtatt agcctgtttc
    15001 ttatactgca tgcattttcc agcggaacga ctgcgctcac cggtgtggag gcaatctcaa
    15061 atggtattac ggccttcaag gaaccccgca gtcgaaatgg gaacattacc ctcacctggc
    15121 tggctctcat cctgagcttg ttgtttcttg gaatatcatt ccttacgcgc gagattcagg
    15181 cagtgccatc agaaaaagaa acggtgattt ctcaaatggc acgtactgtt tttggtggac
    15241 agggactgtt ttacctactt gccattgcag ggaccacctt cattttgatc cttgcggcaa
    15301 acacctcgtt tgctggattt ccgcgtttga gcgcgaagct tgccagtgat ggtttcatgc
    15361 cgcgccaatt gacgtatcga ggcactcgtc tggtgtactc gtatggaatt atctcgctgg
    15421 gggcggccgc gtcgatattg attgtcatct ttcaggcaag cgtaacgcgg ctgatcccgt
    15481 tgtatgcgat tggtgtattc ctgtgtttta cgcttgccca agtcgggatg gcgcgtcgat
    15541 ggaggcggtc aggtcaatta ggaaatgatg agtctacatc gaagtccaaa cagtcaattc
    15601 gatacgatag taagtggatg ttgaagatgt ttgccaatgg cttcggagcg atttgcaccg
    15661 cggttgttat gatcgtcttt atcattacta aatttgtgga tggcgcatgg ataattttgg
    15721 cggtcactcc catctttatc agcgttttgc tttgggttca tcgtcattat aaaagcatgg
    15781 cacagggtct ctccctggaa aactatgggg aaccgccacc ttataatctc cgccatcgag
    15841 ttattgtgcc aatcagcaat gtgcatcagg gtacgctggc tgcgctccgc tatgcccgtg
    15901 ctttatcaga tgatatcacc gctgtacatg tgtccatcga acctgaagac acagaaaaaa
    15961 ctgtaaagaa atgggaaaca tgggggcgtg ggacgcgtct tgtgattgtg gattccccgt
    16021 accgactttt tctggagccc ctgttgacct atattgagga cattatcgcc agccggcagg
    16081 caaacgagac aattacaatt gtggtgccac atttcgt
//
LOCUS       DASTJP010000772         7956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1125908, whole genome shotgun sequence.
ACCESSION   DASTJP010000772 DASTJP010000000
VERSION     DASTJP010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125908"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /gene="xseA"
                     /locus_tag="VFQ23_22640"
     CDS             <1..791
                     /gene="xseA"
                     /locus_tag="VFQ23_22640"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET9909462.1"
                     /translation="FPRKIGIVTSATGAALRDMLNTLIRRLPLVEVILAPSPVQGVEA
                     PPALIKAIRALNDRSPDVIILARGGGSIEDLWAFNDERVVRAVAASDAPVICGVGHET
                     DFTLSDFAADLRAPTPTAAAELATQITIVDLSAELQSYGSRLVSATLNLVGEKRVELS
                     SLQARLRYISPDRRIQSERQRVDELTRRIYSSLTHHLQLQSSHVKGMQKRLDALNPMA
                     VLARGYAVVTRKEDGNVVSRVEQASEVMNVRVIDGEFEVTKNSR"
     gene            824..1075
                     /locus_tag="VFQ23_22645"
     CDS             824..1075
                     /locus_tag="VFQ23_22645"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET9909463.1"
                     /translation="MPKSSNQKTEKTIDELSYEEALAELEEIVSALEGEQNQLEDSIR
                     LFERGQALAARCGVLLEAAELKVKQVVGEDILPFEEESE"
     gene            1072..1524
                     /locus_tag="VFQ23_22650"
     CDS             1072..1524
                     /locus_tag="VFQ23_22650"
                     /inference="COORDINATES: protein motif:HMM:NF014711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HET9909464.1"
                     /translation="MNLLALFENKALIAGLIAWLLAQIIKIPLDYFRTRRWNWALLLT
                     TGGMPSSHSSLMTATTLAIGLYDGFDSPVFALGVAITMIVTYDAAGVRQQAGIHAKRI
                     NVLFDELLHGHPINQRDLREVIGHTPLEVAGGILLGLIVATSQWMFWQ"
     gene            1557..2726
                     /locus_tag="VFQ23_22655"
     CDS             1557..2726
                     /locus_tag="VFQ23_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909465.1"
                     /translation="MKNSIFALIGLLVLFVSACAPMLESTQEALSTQTPPVTTSTKPT
                     GALLIWQSNDSPCKTAAFSTESLSYGECGKALTASTPETADYESRLLELSDSFASFTA
                     ETSVGSLILKGSGESVPTSVEKRAIAEWATWMFETSESGQADTSSSQAFVWQREGGIA
                     GFCDNVSVHLTGLVTTSDCKGFHAEAYLTASQLEQLYGWVYNLGDIEYDDSNAPLADG
                     MQITLSLDGTGQGEPDEQTIRDMIDFASTLDVQLGNAAEAGPEVSDAQNALGEYLLAL
                     HKGDYPRASELYSGDLSLLQTWNPDIPNAGSELFERACSQNGLQCLAPRSITYRSSEV
                     DGHHFWVEFNNNDGTLFQQGPCCGETNGPLISRFPFYVEQRQSGFFVWDLPPYVP"
     gene            3214..3987
                     /locus_tag="VFQ23_22660"
     CDS             3214..3987
                     /locus_tag="VFQ23_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909466.1"
                     /translation="MRTIKSSFRLVTAIFLIAILVSIPAPAKADHSFLGLHWPREENP
                     FRLRFGDNVSSGWDSHLRETAEEWSESRVLNLRVVEGSTTGRRCIPRQGRVEVCSFAY
                     GDNGILYTSTVFFVDDHILGGIIQLNDTYLNSAPFNTRPWRRYILCQQIGFTLGLDYQ
                     DVDPFNTNNGSCNDLTDNPAGPPRNTKPNRHDFDMLKQIYSHSDGDDDLALTSVLSPS
                     IDAGAMDTTAMAASDGNSGVYITDLGNGMMTVTLVGRAK"
     gene            complement(4056..5651)
                     /gene="murJ"
                     /locus_tag="VFQ23_22665"
     CDS             complement(4056..5651)
                     /gene="murJ"
                     /locus_tag="VFQ23_22665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET9909467.1"
                     /translation="MSQTEANRQIARAAGTVMFAIVFGQLAGLLRGVLVARAFGASPE
                     LDAFFAANRVSETLFLLVAGGALGSAFLPTFTGLLAKAERDSAWRLASTLAKVVTLTL
                     SLLAFLLAIFAPQVVRYALAPGLSANTELFSLTISLLRIQMISAVLFGLGGLIVGILN
                     AHQVFLIPALTPALYQFGIIFGAVVLAPSMGIYGLAWGVVIGALMYLVIQVPRLIRLF
                     PQPFPSSNLSNVRDPNTRQVLLLMLPRLLGVAVVQLNFWVNTNLASTMEEGSVTGLYY
                     GFSLMLMAQAAIAQSVAIAAMPTFSAQHALGKTDEIRSSLAASLRGILMMSLPASIGL
                     ILLREPIISFLYQRGEFNEHDVQLVAWALLWYAAGLVGHSIMEVLTRAFFAQQDTRTP
                     VLIGTIAMALNVVFSLWFSSLFQQIGWFPLGGLALANSLATALEATALFIVMRRRLNG
                     IEENHILRGFIAFALAALGMGLTLLFWLEVSAGLMLWLAALGGVALGGLVYGLLLFVL
                     RVPEIQSITQFFLRRLHRFQRVD"
     gene            5726..6823
                     /locus_tag="VFQ23_22670"
     CDS             5726..6823
                     /locus_tag="VFQ23_22670"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HET9909468.1"
                     /translation="MSTITQHGYLLIADISGYTSFVAKTELEHSHEILSDLLGLLVEK
                     FQPLMTISKLEGDAVFAYAAETVFTRGETLIEFIESMYVAFRDKQVSMKRATTCTCNA
                     CRNIPTLDLKFIAHHGDFIIQNVLNIRELVGSDVNLIHRLTKNHVSDATGWRAYMMFT
                     EKCLDYLEMNLDDTHVQLEDYEHLGEVKTFSVDLHKRYKEITESRRILVDERDADLVL
                     SVDFPTPPAVTWEWLQDPTKRNIWSGGHIQWSLGERPGGRAGKGASNHCAHGKTLSTE
                     VTLDWRPFEYSTSESFENGKKTFSETFRFEPLPDGGTRVIDVMQMHMPLPRFLRRLLA
                     KMVLLGQHHYDDQMRKAAQLAGEEFARAKIE"
     gene            6889..>7956
                     /locus_tag="VFQ23_22675"
     CDS             6889..>7956
                     /locus_tag="VFQ23_22675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9909469.1"
                     /translation="MNRLAQLLSIKQGEGRTAFLTIGVMLFTSIGAALGGTGIEALFF
                     ARFGVEYLPYMFVGLGITSMIMSFGVTALLSRIPRQILYITVPLLISILLILARLALL
                     TGVNWLYPVLWLGKEVLNILVGLVIWGIAGVVCDTRQAKRLFPLFNASRILGQVIGGF
                     ITGLLVASIGTENLLLVWSGTLFVAFLLSRSLLAGTQISSAPERRSRRKQPRLIQEMQ
                     RGYQFVRGSKLMTWIAISTTFFSILYFSISLPFSRAATEQYINEDSLASFLGLFNGLS
                     TAAAFLASLLLANRLFARVGIMACILALPIIYLIGFGALAIVPVFIVIVSFRFVQMLW
                     LSGIADPAWQTMFNVVPPEKRD"
BASE COUNT         1948 a         1936 c         2061 g         2011 t
ORIGIN      
        1 tattcccccg caaaattgga attgtcacct ctgccacagg cgcggcgttg cgcgatatgc
       61 tcaatacgct gatacgtcgt cttccgcttg tcgaagtgat ccttgcgccc tcacctgtgc
      121 aaggagttga agcgccgcct gccctgatca aagccatccg ggccctcaac gaccgatcgc
      181 ctgatgtaat cattctggca cgcggcggag ggtccatcga agatttgtgg gccttcaatg
      241 atgaacgcgt cgtgcgcgcg gttgccgcgt cggatgctcc ggtgatttgt ggagttggtc
      301 atgagaccga tttcactctt tcggacttcg ccgctgattt gcgcgccccg actcccacgg
      361 cggcagcaga gcttgccacg caaatcacca ttgtagactt gagcgcggaa ttgcagagtt
      421 atgggtcccg cctcgtttcg gcgactctca atctggtcgg tgaaaaaaga gttgaactct
      481 cctccctgca ggctcggttg cgatatatct ctccggatcg ccgtatccag tctgaacgcc
      541 agcgcgtgga cgagctgacg cggcgcatct attcttcatt gacccaccat cttcaattac
      601 aatcatccca cgtgaaagga atgcaaaaga ggctggacgc gttaaatccc atggcagtcc
      661 tggcgcgtgg atatgcggtg gtcacccgta aagaggatgg taacgtcgtt tcccgcgtgg
      721 agcaagccag tgaggttatg aatgtaagag tgattgatgg tgagtttgaa gtaacaaaaa
      781 attcgcgctg agaataaacg aagcgctcga tataggaacc attatgccaa aatcatccaa
      841 tcaaaaaacc gaaaaaacaa ttgatgaatt atcgtatgaa gaagctttag ccgagctgga
      901 ggagatcgtc tccgcgctgg aaggggaaca gaaccagctc gaagattcga tcaggttatt
      961 tgaacgaggg caggcactgg ctgcgcggtg tggggtgctt ctcgaagccg cagagctcaa
     1021 agtcaagcag gtcgtcggtg aggatatact tccgtttgag gaagagtctg aatgaatttg
     1081 ctggcactat ttgaaaacaa agccctgatt gccgggctga tcgcctggct gcttgcacag
     1141 atcataaaaa ttcctctgga ttattttcgt acacgtcgtt ggaattgggc cttgctcctc
     1201 accacaggtg gcatgccaag ttcacattcc tctctcatga cggccacaac actggcaatt
     1261 gggttgtatg acgggtttga tagtcccgtg tttgcactgg gggttgccat aaccatgatc
     1321 gtgacctacg acgctgcagg tgtccgtcag caggctggca ttcacgcgaa gcgcatcaac
     1381 gtgttatttg atgagctatt acacggtcac cctatcaatc aaagggattt acgggaagtg
     1441 atcggtcata cacccctgga agtcgcggga ggcattctcc tggggttgat cgtcgccacc
     1501 agtcaatgga tgttttggca gtgaaagctt gttatatcga atggttttcg taaattatga
     1561 aaaatagtat ctttgcactt attggactcc ttgtattatt tgtttctgcc tgcgcgccta
     1621 tgcttgaatc cactcaggaa gccttgtcta ctcaaacgcc accggtcacg accagcacaa
     1681 aaccgacagg tgctttgttg atttggcaga gcaacgattc accgtgcaag acagccgcat
     1741 tttcaacgga gagtctttca tatggtgaat gcggaaaggc actcactgcc agcacaccag
     1801 aaaccgcaga ttatgaatcg cgtctcctgg aactttcaga ttcatttgct tcatttacag
     1861 ctgagacgtc tgtcggtagt ctgatcctaa aaggctccgg ggaatctgtt cctactagcg
     1921 tcgaaaaacg ggcgatcgcc gaatgggcca catggatgtt tgaaacctca gaatctggac
     1981 aggcagacac atcaagctct caggcattcg tatggcagcg cgaaggaggc atcgcgggat
     2041 tttgcgataa cgtttctgtg cacctgacag gcctggtaac aacctctgac tgcaaaggct
     2101 tccatgctga agcgtatctt acggcctcac aactggaaca actttatgga tgggtgtata
     2161 acctgggtga tatcgagtat gacgacagca atgcccctct tgctgatgga atgcagatca
     2221 cactgtccct ggatgggacg ggtcagggcg agccggatga acaaaccatt cgcgacatga
     2281 tcgactttgc ttcgacactg gatgtgcaat tgggaaatgc cgcggaggct gggccggaag
     2341 tcagcgatgc tcaaaatgcc ctgggtgagt atctgcttgc tctccataaa ggcgattacc
     2401 cacgcgcctc tgaactgtac agtggggacc tgagtctttt gcagacctgg aatccagata
     2461 ttcccaatgc tggttcagaa ttgtttgaac gggcctgttc gcaaaacgga ctgcaatgtc
     2521 ttgccccgcg ctcgatcact tatcgcagtt cagaagtgga tggtcatcac ttctgggtag
     2581 aatttaacaa taatgatggc acgttgttcc aacagggtcc ttgttgtggc gagacgaatg
     2641 gtccgctgat tagcagattt ccattttatg tagaacaaag acagagcggc ttttttgtgt
     2701 gggatttgcc gccgtatgtg ccgtagttga ttttttgacc tcctaaaaac atgcttacca
     2761 attctatacc agggtggatg tggtattttt gaaattgcct attgtatttg gttgacaaaa
     2821 gtgctatatt ctttgtgggg atattgagtt cgttaaaagc tgcaaggcga atgcacgcac
     2881 gctagcctac atttactttg cggggttact taattggggt ttctttcttg gcgcgcggtt
     2941 gctgactgat cctcatcccg cccactttcc tgactttcat tgctggaacc catttcgtac
     3001 tttttgttgt ttgtttgctt tttgacagga ggtctcagca gctatcatcg cctcacttcg
     3061 taacgcaggc cgtctttaaa taaagagatt cggttttttt gaaccgaacc ttacaaccag
     3121 gcagaaagga ggtcttatcg gatcaactct gtcatcaact cttacctcca taaagaaatg
     3181 tctctcttcg tacacgcaaa aggagtgtta aacatgcgta caattaagag ttcattccgt
     3241 ctggttaccg cgattttttt aatcgctatt ctggtgtcga tccctgctcc tgcgaaagca
     3301 gaccattcgt ttctcggcct tcactggccg cgtgaagaaa atccattccg gttgaggttc
     3361 ggcgataatg tttcgtccgg ctgggattct cacttgcggg aaacggctga ggagtggtcc
     3421 gagtcaagag tactaaatct tcgggttgta gagggttcta caacaggtag aaggtgtatc
     3481 ccccggcaag ggcgtgtgga agtatgcagc ttcgcctatg gtgataatgg gattttgtat
     3541 acttcgacgg tcttttttgt tgatgatcac atcctgggtg ggattattca actcaacgat
     3601 acgtacctga attcggcacc gttcaacacc cgcccctgga ggcggtacat tctctgccag
     3661 cagatcggct tcacccttgg actggattat caggatgttg atccgttcaa tacaaataat
     3721 ggtagttgta acgaccttac tgataacccc gccggacctc cgcgcaacac caaacccaac
     3781 cggcacgact ttgatatgct caaacaaatc tacagtcatt ccgatggaga tgatgattta
     3841 gcgttgacaa gtgtactcag cccgtcgatt gatgccggtg ctatggacac tactgccatg
     3901 gcagccagcg atggaaattc tggggtctat atcaccgatc tcggaaatgg aatgatgacc
     3961 gttacactcg taggtcgcgc caaataggaa atctaaggtc tcaactgaca ggcgcttggt
     4021 gttaaccaag cgcctatgtt tttaaccaga gtcggctagt ccacccgttg aaatctatgc
     4081 aggcgtctca aaaagaattg ggtaatggat tgtatttcgg ggacgcgtaa gacgaataat
     4141 agtagtccat atacaagacc tcccaaagcg actcctccca gtgccgcaag ccagagcatc
     4201 agccctgccg atacctcaag ccaaaacaat aaggtgagtc ccatgcccaa ggccgcaagc
     4261 gcaaacgcga tgaagcctct aaggatatga ttctcttcga tgccgtttaa tcgtctacgc
     4321 atgacgatga agagcgcggt cgcttcgagg gcagttgcca gtgagtttgc cagagcaagc
     4381 cctcccagtg gaaaccagcc aatttgctgg aagaggcttg aaaaccacaa actaaacact
     4441 acattcaatg ccatggcaat tgttccaata aggacggggg ttcttgtatc ctgctgagca
     4501 aaaaacgcgc gagtcagaac ttccataatg gagtgcccga cgagtcctgc cgcataccaa
     4561 agcaaagccc aggccactaa ctggacatcg tgctcgttga attcgccacg ctggtagaga
     4621 aatgagatga tgggttcacg taaaagaatt aatccaatgc tggctggcag cgacatcatc
     4681 aggataccgc gcagcgacgc ggcaagcgaa gaacgtattt catcagtttt gccaagggca
     4741 tgctgcgcag agaatgtcgg catggcggca attgccactg attgagcgat cgcggcctgg
     4801 gccataagca tcaacgagaa accataatac aggccagtca cactaccttc ctccattgtg
     4861 gaggctagat tcgtattgac ccagaaattg agttgcacca cagccacacc aagcagacgg
     4921 ggaagcatga gaagaaggac ctgacgtgtg ttggggtcgc gtacgttact taagttcgac
     4981 gaggggaagg gctgagggaa caagcgtatc aacctgggaa cttgaataac cagatacatc
     5041 aacgcaccga tcacgacgcc ccaggctaat ccgtaaatac ccatggacgg agcaagcacc
     5101 accgcgccaa aaatgattcc aaattgatac agggcggggg taagggcggg aattaagaaa
     5161 acctgatggg cgttgagaat cccgacgata agaccaccga gaccgaaaag tacggcggat
     5221 atcatctgaa tacgaagaag cgaaatggtt aaagagaata gctctgtgtt tgcagataaa
     5281 ccgggcgcga gtgcataccg aactacctga ggggcgaaga ttgccaataa aaaggcaagc
     5341 aggcttaggg tcagtgtgac gactttcgcg agggtagagg caagcctcca ggcggagtct
     5401 ctttcagcct ttgccagtaa ccctgtaaat gttggtagaa aggcggagcc caaggcgccg
     5461 cctgcaacca gcaggaatag agtctcactc acgcggttgg cggcaaagaa ggcatccagt
     5521 tcgggagatg ccccgaaggc ccgggcgaca agaaccccac gcagtaatcc ggcgagttga
     5581 ccgaatacga tagcgaacat cactgtaccg gctgcgcgag cgatctgccg atttgcttct
     5641 gtctgcgaca tgtccgacga ttatactttc ataacggccg ccagttggaa cagatggtcg
     5701 gagtataatc actgcgaaat tcataatgtc cacaatcacc caacacggct atttgttaat
     5761 tgcagatatc tctggctata cttcctttgt cgccaaaaca gagttggaac actcacatga
     5821 aattctctct gacctgcttg gtttacttgt agagaagttt cagccgctca tgaccatctc
     5881 caagctcgag ggggatgcgg tcttcgcata tgcagccgaa acagtgttta cgcgcggtga
     5941 aacgttgatt gaatttatcg agtctatgta tgtggcattt cgcgataagc aggtttcaat
     6001 gaagagagcg acaacctgca cttgcaatgc ctgtcgtaat atccccactc tcgatttgaa
     6061 gttcattgcc catcacggcg attttattat tcagaacgtg ctgaatatcc gtgagctggt
     6121 tggttcggac gtaaatttaa ttcatcgact cacaaagaat cacgtctctg atgcgacggg
     6181 ctggcgagcc tatatgatgt tcacggagaa atgcctggac tatcttgaga tgaatttgga
     6241 tgatacgcac gttcaactag aggattacga gcatttaggg gaggtcaaaa ccttcagtgt
     6301 tgatttgcat aagcgttata aggaaattac agagtcgcga cgcattttgg ttgatgagag
     6361 agacgccgat ctagtcctca gcgttgactt tcctacgcct ccagcggtga catgggaatg
     6421 gcttcaggat ccgaccaaac gcaatatatg gtctggtgga cacatccagt ggtccttggg
     6481 ggagagaccc gggggacgcg caggcaaagg tgcaagcaac cattgcgcgc atggaaaaac
     6541 gttgagcacg gaggtgactc tggactggcg tccgtttgaa tattcaacga gcgagtcgtt
     6601 cgaaaatggt aagaaaacct tttcggagac gtttcgattt gagccgttgc ctgatggcgg
     6661 aacgcgcgtc attgacgtga tgcaaatgca tatgccattg ccacgcttcc ttcgtcgtct
     6721 cctcgcgaaa atggttctcc tgggacaaca tcattatgat gatcagatgc ggaaagcggc
     6781 ccaactcgcg ggtgaagaat tcgcaagagc gaaaatcgaa tgaatgtgga tgaaacccag
     6841 cgtggcgatt tgtttttgtc caaattttcc aaatgctgac gtaaacagat gaatcgactc
     6901 gcgcaactcc tttccatcaa acagggtgaa ggccgaaccg cattcctcac gatcggagtc
     6961 atgctcttca cctcgatagg tgcagccctg ggcgggactg ggatcgaggc gttattcttc
     7021 gcacgttttg gcgttgaata tctgccgtat atgttcgtag ggctgggtat tacttccatg
     7081 atcatgtcgt ttggggtaac ggctctcctt agccgaattc cccgccaaat tctgtatatc
     7141 actgtcccat tgcttatttc tattcttctg atcctcgccc ggttggcact tctcacagga
     7201 gtcaactggc tgtatccggt gttatggctc gggaaggaag tgctcaacat tctggtcggt
     7261 ttggtgatct ggggaatcgc cggagtggtt tgtgatacgc gtcaggcaaa gcggctgttt
     7321 ccgttattca atgcttcgcg cattctgggt caggtgattg gcgggtttat caccggcttg
     7381 ctggtcgctt ccataggaac tgaaaacctc ctcctcgttt ggtcagggac attgtttgtc
     7441 gcatttctgc tcagtcgttc tctgcttgcc ggaacccaga tttcatctgc accagagcgt
     7501 cgctcccgtc gtaaacaacc cagactgatt caggaaatgc aacgcggtta tcaatttgtg
     7561 cgtggctcga agctgatgac ctggatcgcg atctccacga ctttcttttc gatcctttac
     7621 ttttcaattt ccctgccgtt ctcacgcgcc gcaaccgagc aatatatcaa cgaagattca
     7681 cttgcatctt tcctgggtct tttcaatggt ctcagcacgg ctgcagcttt tttggcttct
     7741 cttctgcttg caaacagact ctttgcgcgg gttggcatca tggcatgcat tctggcactt
     7801 cccatcattt acctgattgg ctttggcgcg ctggcgatcg tgcctgtctt tatcgtgatc
     7861 gtcagttttc gttttgtgca gatgctctgg ctttcaggga ttgctgaccc ggcctggcag
     7921 acgatgttta atgttgttcc gcctgaaaaa cgcgac
//
LOCUS       DASTJP010000773         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_491692, whole genome shotgun sequence.
ACCESSION   DASTJP010000773 DASTJP010000000
VERSION     DASTJP010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491692"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFQ23_22680"
     CDS             complement(<1..613)
                     /locus_tag="VFQ23_22680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9909470.1"
                     /translation="MTEQIKKQPLLSGLLILFLAAMIFANIGGNMYQSFMPLYVKDLG
                     ASIRQIGLFFTLAQIVPLFLQILGGWISDSLGRLRAIAIGSVFGVLGFIPMVLADRWE
                     WLLFAMAVGSIARALVGPSFDAFIAEHSSEENRGKVFGISQAIFMIVSVVGPPLGGWL
                     AGSYGFRLMLLVAGIFYFIAMVIRLGMAREAAKSETSATQKLSF"
     gene            complement(626..1261)
                     /locus_tag="VFQ23_22685"
     CDS             complement(626..1261)
                     /locus_tag="VFQ23_22685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909471.1"
                     /translation="MSSEERKKILQMVAEGKISPTEAATLMRAMDADSDPVEEEMEVI
                     QTAAGQSSESTAAPEFEEIKARARRFSLIPLWVGVFITVISAWIIYSIQQNGGTGFWF
                     YCMVLPLMFGILLITLGAGGRSSRWLYVDVDRRNAKAGDGPRHITLGFPLPLGFIAWF
                     FRTFGSNIHGMSGGRIEGIIQLMNATKDSNEPFIVNVDEGGNGEHVRVFIG"
     gene            complement(1261..2370)
                     /locus_tag="VFQ23_22690"
     CDS             complement(1261..2370)
                     /locus_tag="VFQ23_22690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909472.1"
                     /translation="MSKSIHVGSSPRININAVGGDLSIVGWDGEDLLVKADDDDTSVE
                     NKNGEVTFVSGGDLSLRIPKAAYLLLKSAGGDVAIRGVIGDIELKDIGGDLSIREAGS
                     IVVDTIGGDFSLRSAKKNLYVKNAGGDVSIREVEGSVTLDSVGDDLALRGARGNIKVN
                     VGEDVVVYLEPKPDGEYSITAGDDILLVLPTNANATLNIKGDRILVDWPSFKDEDETE
                     RIVTLGNGSAKISLNAGGKVRVSNNAEAGESAEEFGNFAGLNFDWSGFGERISKQVEQ
                     ATSRAAKRVEEAAKRAERHAERVAGRHMRHGKHGLSVGPWNWDLGPKGVPTPPSPPSE
                     PVSEDERMAILKMLAEKKITAEQAEQLLAALEGGK"
     gene            complement(2373..2756)
                     /locus_tag="VFQ23_22695"
     CDS             complement(2373..2756)
                     /locus_tag="VFQ23_22695"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HET9909473.1"
                     /translation="MFAAPTRCPVCRSELEIARLHCPSCDTSIEGRFTGGPIAHLTAE
                     HLDFIVTFVRCEGKINRMEQELGLSYPTIRNRLHEVIRALGFEPGKDEPVEISSEKRS
                     SVLEDLETGKISADEAMRMLRGEEA"
     gene            complement(2794..3033)
                     /locus_tag="VFQ23_22700"
     CDS             complement(2794..3033)
                     /locus_tag="VFQ23_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909474.1"
                     /translation="MDNMFPDRLEDLNRQKIDKDRAFILKEEMLKSRSGLNTFLNGLG
                     SWMVSTGEDLRKKNAASAQVRKLDSLQEASRIFKA"
BASE COUNT          843 a          872 c          668 g          831 t
ORIGIN      
        1 cgaaagaaag cttttgagtg gcagacgttt cacttttcgc ggcttcacgt gccattccga
       61 gtcgtatgac catcgcaata aagtagaaaa tacccgcaac caatagcatc aatcgaaacc
      121 catatgaccc cgccagccaa ccacctaacg gcggacctac taccgagaca atcatgaaaa
      181 tcgcctgtga gatgccgaat actttgccac gattttcctc gctggaatgt tctgcaataa
      241 acgcgtcgaa acttgggccc acgagcgctc gtgcaattga accgacagcc atcgcaaaca
      301 gtagccattc ccatcgatcg gcaaggacca ttgggataaa accgagcacg ccaaacacac
      361 ttccaatggc gatggctcgt aatcgcccga gagaatcaga aatccagcca ccgaggatct
      421 gcagaaacag agggacaatc tgtgccagtg taaagaacaa tccgatctga cgaatcgatg
      481 cacccagatc cttaacataa agcggcatga acgattgata catgttcccg ccgatgtttg
      541 caaagatcat ggcagcgaga aacaaaatca aaagtccgct taataaaggc tgtttcttaa
      601 tttgctcggt cattattgtt cgccgttaac ctataaatac gcggacgtgt tcaccgttac
      661 cgccttcatc aacatttact atgaatggct catttgagtc tttggtcgca ttcatgagtt
      721 gaatgattcc ctcgattcgt ccgccgctca taccatgaat gttggatcca aacgttcgga
      781 aaaaccaggc aataaatccc aggggtaatg gaaatcccag cgtgatatgc cgtggaccat
      841 ccccggcttt tgcattgcga cggtcgacat ccacatagag ccatcgtgaa gatcgtccac
      901 ctgcaccaag cgtgatcaat aaaattccaa acatcaatgg cagcaccatg caataaaacc
      961 aaaaaccggt gccgccattt tgttggattg aataaatgat ccacgcgctt atcaccgtga
     1021 tgaacacgcc cacccataac gggatcaggg aaaaacgtcg agcacgtgct ttgatctctt
     1081 caaattccgg cgcagcggtg ctttcggaac tctgtccggc tgccgtttga atgacttcca
     1141 tctcctcttc cacgggatct gagtcagcat ccatggcccg catcagagtt gcggcttctg
     1201 taggggaaat tttcccctct gccaccattt gaaggatttt ctttcgctct tccgaagaca
     1261 ttattttcct ccctccaacg ccgccagcaa ttgctcagcc tgctcggcgg ttattttctt
     1321 ctcggccagc atttttagaa tcgccatacg ctcatcttct gaaacaggtt cagacggagg
     1381 agatggggga gtcggcacgc ctttcggacc aagatcccag ttccaggggc ccactgacaa
     1441 accatgtttc ccatggcgca tatgccgtcc ggcaactcgt tctgcatgac gctcggcgcg
     1501 cttggccgct tcttccacgc gctttgccgc gcgggatgtg gcttgttcga cctgcttcga
     1561 tattctttcg ccaaagccag accagtcaaa gttcaagccc gcaaaattgc cgaattcttc
     1621 agcggattca cccgcctcgg cattgttgga aacacgtacc tttccacccg cgttcaacga
     1681 gattttggca gaaccatttc ccaatgtgac aatacgttcc gtttcatcct catccttaaa
     1741 gcttggccaa tctacaagaa tacgatcacc cttgatatta agtgttgcat tggcattggt
     1801 cggcaggacc agcaggatat catcaccggc ggtgatggaa tactcaccat ccggttttgg
     1861 ctccaaataa acaacaacat cttcgcccac attaacctta atgtttcctc gagcgccgcg
     1921 cagtgccaga tcatcgccca ctgaatccag cgtgacgctg ccttccactt cacggatgga
     1981 aacatctccg ccggcgttct tcacataaag atttttcttt gcgctgcgca gactgaagtc
     2041 gccgccaatc gtatctacga caattgaccc tgcttcgcgg atcgaaagat cgccaccgat
     2101 gtcttttaat tcaatatcgc cgatgacgcc gcgaattgcc acatctccac cggcagattt
     2161 tagaagaagg tatgccgctt ttggaatgcg cagcgacaaa tcgcctccac tgacaaatgt
     2221 cacttctcca tttttgtttt cgacgctggt atcgtcatca tccgctttca cgagtagatc
     2281 ttcaccatcc caaccgacga tactgagatc cccacccacc gcattgatgt taattcttgg
     2341 agatgatcca acatgaattg atttgctcat aatcatgcct cctcgccgcg caacatgcgc
     2401 atggcttcat ctgcagatat tttgcctgtt tccaggtctt caagcacact gcttcgtttt
     2461 tcagaactga tctccaccgg ctcatccttt cccggttcaa agcccagagc acggatcact
     2521 tcatgcaaac ggtttcgaat ggtggggtag gagagaccta attcctgttc catgcgattg
     2581 atcttcccct cgcatcggac aaaggttacg ataaaatcta aatgttcagc tgtaaggtgt
     2641 gcgatcggtc cgccagtgaa gcgaccttca atggaagtat cacaagacgg acaatgtaat
     2701 cgtgcaattt ccaattcaga acgacaaaca ggacaacgag tcggtgcggc aaacatagaa
     2761 cctccactta gtcgggcttg taatgataat acttcatgct ttgaaaattc tggatgcctc
     2821 ttgaagagaa tccaatttgc ggacctgagc cgatgcagcg tttttctttc gcaggtcttc
     2881 gccagtgctg accatccagc ttcccagccc gttcaggaag gtattgagac ctgacctcga
     2941 tttcagcatt tcttccttga ggataaatgc ccgatcttta tcaattttct ggcgatttag
     3001 atcttccaaa cgatctggaa acatattgtc catagttttc tccttgattt taggcaaatt
     3061 tgagccaaaa atcaattttc tcaaagccaa aatcacgttt catgatgtta ttatattcat
     3121 aatttataat ttgtcaagta aaactaaata aaataaagaa atttattaag atatttaaac
     3181 ccagatttag aatcttaaac cccttttttg gagg
//
LOCUS       DASTJP010000774         6361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1148980, whole genome shotgun sequence.
ACCESSION   DASTJP010000774 DASTJP010000000
VERSION     DASTJP010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148980"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..554)
                     /locus_tag="VFQ23_22705"
     CDS             complement(171..554)
                     /locus_tag="VFQ23_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909475.1"
                     /translation="MANSMMILIAGPYRSGTGDDPEKMAANVHEMETYALPIFRLGHI
                     PVLGEWFALPLVTLAGSTQVGDEAFNEIFHPIAERLLSKCDAVLRVGGASQGADLMVK
                     TAKEKGLQVYYSLEEIPAGSHQDFK"
     gene            complement(547..1086)
                     /locus_tag="VFQ23_22710"
     CDS             complement(547..1086)
                     /locus_tag="VFQ23_22710"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9909476.1"
                     /translation="MPFELIKSETLLQGRVFKIRRDFLRTPEGRETRLEIIEHGGSVV
                     IVPIDQEGNVIFVRQYRHAAGGDILELPAGTRESNEPHEECAARESREEIGMEAGKLE
                     KVGDFYLAPGYSTEFMAVFIATELKENPLDPDEDEVLEVEKIPAGKVFDLAKAGKIQD
                     AKSLAALLLAKPQLEKYLG"
     gene            complement(1174..1878)
                     /locus_tag="VFQ23_22715"
     CDS             complement(1174..1878)
                     /locus_tag="VFQ23_22715"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9909477.1"
                     /translation="MARITRGTTTASILMLVALVVSSCNQPYSQQPSVTNTPIDTSLF
                     ATPIGQTPAISMSDVANFGTQTAIAQTTTPGIPAATLDPALATATATSNIAITPQETF
                     TATQAVVGAGTTLPTSAPVGSRPSSYTLQRGEFPYCLARRFDVNPEELLSLNGLSDGT
                     IYYPNLSLRIPQSGSFPGTRALRNHPATYTVAASSETVYSVACVFGDVDPAAIAQANG
                     ISVGTALTSGQTLSIP"
     gene            complement(2068..4104)
                     /locus_tag="VFQ23_22720"
     CDS             complement(2068..4104)
                     /locus_tag="VFQ23_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909478.1"
                     /translation="MQAKRFLERNIPLLFLIVTIVAYGLLLPLTGFYWDDWPFLWIAE
                     FLGPQEFFPAFAAVRPFLAPIFFITTSLIPPIPIYWQLFALVIRFLSGLSAWFALNQV
                     WPNHKRQILIASLLFLVFPGYSQHWVALTHINQEWIPFIFYLLSFGFTARALRNIERF
                     KTNTYFALLFLILGVFPTEYFVSMEPMRFLFIWVIVSENIQGFQQKLIQSLKYWWPYL
                     LIWLANAGWLAYFYTIGSYESYDVEVVKQPLTLLHLFSTMGEAIWKAGFYVWGQVLVL
                     TSEAIAAPTSLLTIALILLSFFLLLFYLSKYTFPQTSTLQFAVQAIIIGLLGILLGRI
                     PSFAAGLPLTLQSSFDRLMISIMLGASLFAIGLIELLIKNIRIKTYIFALLIALGIGQ
                     QFFNANIFRRDWAQQQQIYWQLAWRIPAMERDTVLLTDQMPIDYETDLSFTAPINWMY
                     MPDYKRSTPQSGSVDKRSNLPYGLLYVEKRLGGSLLSLEPNTKITLDFRTVDFVGSTS
                     QAIVIHMPRNGCLRVLDPARGDAITYERQSRFLVDAIPLSNPARIDTNSDRTATLPFL
                     SEPTHTWCYYYAKSELARQKEDWTQVVDLMDEAIALDYRPEDPFEWLVYIEAKGMIGD
                     VEDAEKLSSDAFDQEKGIRNGLCIVWKRIQTQVEAGNASAQRINQVLTDYRCAQ"
     gene            complement(4139..5176)
                     /locus_tag="VFQ23_22725"
     CDS             complement(4139..5176)
                     /locus_tag="VFQ23_22725"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     response regulator"
                     /protein_id="HET9909479.1"
                     /translation="MKPAHEPGHLLVVDDNKVNRILLSRGLEQQGHKVDTAENGRQGL
                     EKLRANFFDLVLLDIEMPEMNGYQVLETCLQDPDLRDIPIIMTSSLDEINSVVKCIEL
                     GAEDYLNKPVNPILLRARVNASLEKKRLRDEQRKLFRTFATKEVADELLRSGFSLGGE
                     YVDASVMFADIRSFTSIGEKQEPADTIELLNNYFALMFEAIVNNHGTVNQLEGDGFMA
                     IFGAPIYYEEHREHVVRAALEMTELLKVFNAEQATQNKAQIKIGIGIASGRVIAGYTG
                     TQHRATYTCVGDTVNIAARIEKYTKEAKRPILIDLDTRDGLPEGVEVETLGPVLFQGK
                     QFPIHIFAVNG"
     gene            complement(5173..5508)
                     /locus_tag="VFQ23_22730"
     CDS             complement(5173..5508)
                     /locus_tag="VFQ23_22730"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9909480.1"
                     /translation="MPVIDKATFEELKQMSGADFINELIDAFLDDAPNMMQNMQAALE
                     IKDIESFRRNAHSLKSNANTFGATELGVRAKELEFMAKENNLDVGTKLEGLKEEFDKA
                     AQELRGMRE"
     gene            complement(5571..>6361)
                     /locus_tag="VFQ23_22735"
     CDS             complement(5571..>6361)
                     /locus_tag="VFQ23_22735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909481.1"
                     /translation="KRVLIVDDNATNRRILSLQTEKWGMTSRETEHPREALEWIRESE
                     NFDLAILDLQMPEIDGIMLTREIRKLRDEKSLPIILLTSLGRREIGAEDLDFAAYLIK
                     PLKPSALYDALAGLFATNVISPKVDSIKPVMDADLGKRHPLRILLAEDNVVNQKLAVR
                     LLELMGYRADVASNGLEAVESIQRQIYDVVLMDVQMPDMDGLDATREIRKLIEVTQPH
                     IIAMTANAMEGDREMCIAAGMDDYISKPIRVNELVDALVKAERK"
BASE COUNT         1672 a         1529 c         1496 g         1664 t
ORIGIN      
        1 gattacgaga gccttttttt gtttaacttc gttatccgtt ggtcgagtac gcgttgtagt
       61 caatagaaaa tccctttgga tctggtggtc gagtaatccc gagggtttgt cgagggatgt
      121 atcgagacca ccggatgccg ccagattctt aaaactgtca ggtcactaaa ttacttgaaa
      181 tcctggtgag aaccagcagg aatctcttct aaagaatagt agacctgtaa tcccttttcc
      241 ttcgccgtct ttaccattaa atctgcaccc tgtgaagcgc caccaactct caagactgca
      301 tcacatttgc tcaacagtct ttcagcaatg ggatgaaaaa tctcattgaa cgcttcatcc
      361 ccgacctgtg ttgaaccagc cagcgttaca agcggcagcg caaaccattc ccccaatacc
      421 ggtatgtgcc ctaaacgaaa aatgggtaag gcgtacgtct ccatttcatg tacattcgca
      481 gccatctttt ccggatcatc ccccgtgcca gaacgatagg gtcctgcgat aagaatcatc
      541 attgagttag ccaagatatt tctccagttg aggctttgcc agcaaaagag ctgccagaga
      601 tttggcatcc tggatttttc cggcctttgc aaggtcaaat acttttccag caggaatttt
      661 ttcaacttct agaacttcat cttcatcggg gtcaagcgga ttttccttca actctgtggc
      721 gataaacact gccatgaatt ctgtggaata tccgggtgca agatagaagt cacccacttt
      781 ttccagtttg cctgcttcca tgccgatctc ttcacgtgac tcgcgcgccg cacattcttc
      841 atgtggttca ttagactcac gcgtgcctgc gggaagttca aggatatcac cgcctgcagc
      901 atgccggtac tgacggacaa agatcacgtt gccttcctga tcgatcggca caatgacaac
      961 ggaaccaccg tgttctataa tctcaagtct ggtttctcga ccctcaggtg ttctcagaaa
     1021 gtcacgtcga attttaaaaa cacggccttg caagagggtt tcggatttga ttaactcaaa
     1081 aggcatgata catttccttg atctggttgt ttctcgaatt ataaacaaaa gcccaggcaa
     1141 ttgcctgggc tttcattatt atgaaaagat caactacggt atgcttaacg tttgcccgga
     1201 ggtgagggct gtgccaaccg agattccatt ggcctgtgca atggctgctg ggtcaacgtc
     1261 tccaaacaca caagccacgc tataaaccgt ttcactggaa gcagcaaccg tataggtggc
     1321 tgggtggttc cgcagagcgc gagtgcctgg gaagctgccg ctttgaggaa ttctcaggct
     1381 gaggtttgga taatagatag tcccatcaga caggccattt agcgatagca attcttcagg
     1441 gttaacatcg aagcggcgcg caagacaata agggaattca cctcgttgca gcgtatacga
     1501 cgaaggacgt gaaccaaccg gtgcagaggt gggcagtgtg gtgccagcgc caacgacagc
     1561 ctgtgttgca gtaaatgtct cctgaggtgt gatggcgata ttggatgtgg ctgttgcggt
     1621 agccagagca ggatctaatg tagccgcggg tattccgggt gtagtcgttt gcgcgatcgc
     1681 cgtctgtgtg ccaaaattag caacatcact cattgaaatt gccggcgtct gaccaatggg
     1741 tgtggcgaac aaacttgtat caatgggagt atttgtgaca ctgggttgct gtgaatatgg
     1801 ctgattacat gaagaaacaa cgagtgcaac cagcataaga atggaggcag tcgttgtgcc
     1861 tcgtgttata cgcgccatag tcttctcctc aatctcgaac aaagtttgcc gcctgtctga
     1921 cgcatgttct ccatgcgcaa ttaaactttt gaaaagatgc caattagaat ccaaagttta
     1981 ggttagaaaa ttatgcggca ctgtgaatcg atggtagcac aaccctttta gcgcgtcaag
     2041 ttatgggatg ggatattaga gggataccta ctgcgcgcaa cgataatcgg tcaatacttg
     2101 attgatacgc tgcgcggatg catttcccgc ctcgacctga gtctgaatcc gtttccagac
     2161 aatgcacaac ccgttgcgga ttcctttctc ctgatcgaat gcatcagacg ataatttttc
     2221 tgcatcttca acgtctccga tcatcccttt cgcttcgata tataccaacc attcaaaggg
     2281 atcttccggt cgatagtcca gagcgatggc ttcatccatc aaatcgacga cttgagtcca
     2341 atcctctttc tggcgggcca attccgattt tgcatagtaa taacaccacg tatgagtggg
     2401 ctcggataaa aaaggcaatg ttgcagttcg atctgaattc gtatcaattc gcgcgggatt
     2461 tgaaagtgga attgcatcca ctaaaaaacg ggattggcgt tcataggtta ttgcatctcc
     2521 ccttgccgga tcgagcacgc gcaggcatcc atttctcggc atgtgtatca caattgcctg
     2581 tgatgttgaa cccacaaagt caacggtacg gaaatccagc gtaattttcg tgtttggttc
     2641 aagcgatagc aaactgccac ccaggcgttt ttccacatat agtaatccgt atggcagatt
     2701 cgaacgcttg tccacactgc cgctttgcgg cgtgcttcgc ttgtaatcag gcatatacat
     2761 ccaattaatg ggtgcagtaa atgaaagatc ggtttcgtaa tcgataggca tttggtcggt
     2821 caggagaacg gtgtcccgtt ccatggcagg aatccgccat gccaactgcc agtagatttg
     2881 ctgttgttgt gcccaatccc tgcggaagat attagcattg aagaattgct gcccgatgcc
     2941 taatgcaatt aacaaggcaa atatataggt ctttattcta atgtttttga ttagtaattc
     3001 gatcaatcct atcgcaaaaa ggcttgcgcc gagcatgatt gagatcatca gacggtcaaa
     3061 gcttgactgg agggtgagag gcaacccggc tgcaaaggag gggatacgcc caagcaggat
     3121 accgaggaga ccaatgatga ttgcctgcac ggcaaactgt agtgttgatg tttgcgggaa
     3181 agtatatttg gataaataaa ataaaagaag aaagaaggaa agaagaatca atgcaattgt
     3241 taggagtgat gtcggcgcag cgatcgcctc ggaggtaagc accagcacct gtccccaaac
     3301 ataaaagccc gctttccaaa tggcttcgcc catggtggaa aaaagatgaa gcaaagtcag
     3361 tggctgtttt acaacttcga catcatagga ctcataagac ccgattgtgt agaaataagc
     3421 cagccagccc gcatttgcca gccagatcag caggtatggc caccaatatt ttaaggattg
     3481 gatcaacttt tgctggaagc cttgtatatt ttcagaaaca attacccaga taaataagaa
     3541 gcgcataggt tccatgctga cgaagtactc ggtggggaaa actcccagta tcaagaagag
     3601 cagtgcaaaa taagtgtttg tcttgaaccg ctcgatgttt ctcaatgcac gggcggtaaa
     3661 gccgaatgag agaagataaa aaataaatgg aatccattcc tggtttatat gtgtcagggc
     3721 gacccaatgc tggctataac ctggaaagac cagaaatagc aaggacgcga tgagaatttg
     3781 tctcttgtgg ttgggccaaa cctgatttaa cgcgaaccag gctgaaagtc ccgaaagaaa
     3841 acgaataacc aatgcgaata actgccagta aatgggaatt ggaggaatca ggctggttgt
     3901 aatgaaaaaa atcggtgcca ggaaagggcg taccgcggcg aaggcaggga aaaattcctg
     3961 cggccctaga aattctgcaa tccagagaaa cggccagtca tcccaataaa aaccggttaa
     4021 cgggagcaac aacccatacg caacaatcgt gacaatcaaa aataaaagcg gaatgttccg
     4081 ctcaagaaaa cgcttagcct gcatacgatg aattataaaa gaaaagccgc gttgggattc
     4141 atccgttcac agcaaatatg tggatcggga attgtttccc ctgaaacaaa acaggtccaa
     4201 gtgtttcgac ttcgacgcct tccggcaatc catcgcgcgt atccagatct attaaaatgg
     4261 gtcttttcgc ttctttggta tatttttcaa tgcgcgctgc gatattaacc gtatcaccga
     4321 cacatgtata cgtggcgcga tgctgcgttc ctgtataacc cgcgattacc ctgccagatg
     4381 caattccgat gccaattttg atctgcgcct tattctgtgt ggcttgttcg gcgttgaaga
     4441 ccttgagcag ctcggtcatc tccagggccg cacgcacgac atgttctcga tgctcttcat
     4501 agtatattgg cgcgccgaag atcgccatga acccatcgcc ctccagttga ttgactgtcc
     4561 catgattgtt gacgatcgcc tcaaacatca aggcaaaata attattaaga agttcaatcg
     4621 tatctgctgg ttcctgtttt tcgccaatgg atgtgaagga acgaatatct gcgaacatca
     4681 cgctggcatc gacatattct ccgcctaagg aaaagcctga tctcaataac tcatctgcta
     4741 cttctttggt ggcgaaggtg cggaacagct ttctctgttc atcccgcaga cgtttctttt
     4801 caaggctggc attgacacgc gcccgcagca aaatggggtt taccggttta ttgaggtaat
     4861 cctccgcacc caactctata cacttgacta cgctgttaat ttcatcaagg gaagaggtca
     4921 taataatggg aatatctcgc aggtcagggt cctgcaaaca agtttcgagg acctgatagc
     4981 cgttcatctc aggcatttca atatcgagta acaccaggtc gaaaaaattc gcgcgtaatt
     5041 tttccagccc ctgccgtcca ttctcggctg tatcaacctt atgcccttgt tgttccaggc
     5101 cacgcgacag cagaattcga tttaccttgt tatcgtcaac caccaacaaa tggcctggtt
     5161 catgagcagg tttcattcgc gcattccccg taattcctgt gcagctttgt caaactcttc
     5221 cttcaagccc tcgagctttg tcccaacgtc caggttgttt tcctttgcca tgaactccaa
     5281 ctccttggcc cgcacgccga gctctgttgc cccgaatgta ttggcatttg atttcaagga
     5341 gtgcgcattg cgacggaatg actctatatc ctttatctca agtgctgcct gcatgttctg
     5401 catcatattt ggcgcgtcat caagaaaagc gtcaattagc tcattgataa agtccgcacc
     5461 agacatttgt ttgagctcct cgaatgtcgc tttgtcaatt acgggcatat aaatctcctg
     5521 ctgtcacatt agctcctctt aacctagaga tgaatcctta tggaagaata ttatttccgc
     5581 tctgctttta cgagtgcatc caccagttca ttcactcgaa tcggcttact gatgtaatcg
     5641 tccataccgg cggcgatgca catttcacga tcaccttcca tggcgttagc agtcatcgca
     5701 atgatgtggg gctgcgtaac ctcgattaat tttcggattt cacgggtggc gtcgagtcca
     5761 tccatatcag gcatctgaac atccatgagc acaacatcat agatttgacg ttggatggat
     5821 tctacagcct ccagaccatt cgatgcaaca tcagctcggt atcccatcag ctcaaggagg
     5881 cgtactgcaa gtttttgatt gacaacatta tcttcagcga gcaggatgcg cagcggatga
     5941 cgcttgccca gatccgcgtc cataacgggc ttaatggaat cgaccttcgg agaaatgaca
     6001 tttgtagcga agaggccggc taaggcgtca taaagagctg atggtttgag aggtttgatc
     6061 aaatatgccg cgaagtccag atcctctgca ccgatctcac gccggccaag agacgtcagt
     6121 agaatgatgg gcagggactt ttcgtcgcgc agtttgcgaa tttcacgcgt caacatgatg
     6181 ccgtcgatct caggcatttg caaatcgagg atggccaggt caaagttctc gctttccctg
     6241 atccattcaa gcgcttcacg tgggtgctcc gtttcgcgag atgtcatacc ccatttttct
     6301 gtttgaagcg agaggatccg ccgattggtg gcattgtcat ccacaatcag gacgcgctta
     6361 t
//
LOCUS       DASTJP010000775         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_702744, whole genome shotgun sequence.
ACCESSION   DASTJP010000775 DASTJP010000000
VERSION     DASTJP010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702744"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1538)
                     /locus_tag="VFQ23_22740"
     CDS             complement(147..1538)
                     /locus_tag="VFQ23_22740"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9909482.1"
                     /translation="MNQEEILSGKAGTAGIRHLLSGQSSRRTLRDEVQSMLRPGYRAG
                     VFDLTRAKFKPARKLSAYFTIPVLDANETSSPPVHLAVTWQNSLDDSAPADQWSQLQS
                     EAEGSGLMPVQSALWRDVPEKGMRLQLWPFDPKFPQLVRLGTPAYVAGVFSSLGIADQ
                     TPLITPIRYRPKERHVLRYEIDSTGAAHSEHQLLYAKLYSNAQDASRAFGVASRVVDW
                     LERNNIGLQGNRPQAISEKDAVIFYPHAPGIPLSRQLHRSRRWLAKQLHIIGKGLLIL
                     HNGPEALQADLKQNNFTSEAKVVRRACEHIQILLPDTYQKILDVVDRAEEQYYGLPQE
                     QPTFTHADFKSDHLLTTPEGLTLIDFDTCTLTDPALDLGKFLADLEWWFTSRGISGVE
                     EAQADLLNSYAAGKESDPNMQARLERAKLFHVLILVKIVARRVPVYKTGWESKTEKLI
                     QRAADVLHQAVAA"
     gene            complement(1535..3025)
                     /locus_tag="VFQ23_22745"
     CDS             complement(1535..3025)
                     /locus_tag="VFQ23_22745"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9909483.1"
                     /translation="MAKTDQPISQEQDALILEGLQIFRELPDWMMAGRDPDRLCAAFS
                     ESIPEFRSGQLILHECDSSNVRYKGENWKGFYELTVSKPGESDKSEIHLDGVLSAPGI
                     NSERPLSVENALGSEDWHAYIPALNLELWTKKPEAVLSSLDMLIDPEQSREYLMSSIR
                     AGSPAYQELQIDATHPKIVRYKPGSRCTIVYNLDYPADAHTHWPELVVAKTYRKEKGQ
                     SAYESMRAFWETALSSSTLVKIAEPLAYDAELKVMIQGPIRQERTLKELTVSAIKAGT
                     AESYDELTEAMRKTARGLAELHNSGTKLTSNYGWEDDEEQTRESIQELSVSVPQLAAA
                     GMPLLERLNSLEASAPPDPRVPSHGTFRPAQVLMYGEEIGFIDFDSSCMAEPAKDLGL
                     FLCAFLRAGMATVKFDDMEVASEPIDHATRVARFERLIGISEKFLEEYERHTGPVNRQ
                     RVALFEALELFILILHAWTKIKVRELNDIMFVLEQFLPAYKILNPK"
BASE COUNT          729 a          809 c          809 g          792 t
ORIGIN      
        1 ccgaaatggc attgacgtaa atggcaacga gagcattgaa ccggtttcag gggagggtgg
       61 cgcgctgact gcctacgaac acgcgtatta catggcagac atgtccatta tccccagttc
      121 gaatcaaacc ccataaaaaa acgaaattaa gctgccacag cttgatgaag tacgtccgca
      181 gcgcgctgaa tcaatttttc ggtcttcgat tcccacccgg ttttataaac cggcacgcgc
      241 cgtgccacga ttttaaccag gattagaaca tgaaatagtt ttgcccgctc caggcgcgcc
      301 tgcatatttg gatcagactc ttttcccgca gcatagctgt taagcaaatc cgcctgggct
      361 tcttcgacgc ctgaaattcc tcttgatgta aaccaccatt caaggtctgc gagaaacttt
      421 ccgagatcca gagccgggtc ggtaagagtg caggtgtcga aatcgatcag cgttaagcct
      481 tcgggagtgg tcagcaaatg atccgattta aagtccgcgt gcgtaaaggt cggttgttcc
      541 tgtggcaaac cataatactg ttcctctgct cgatccacaa catccaggat tttctgatag
      601 gtatcgggca agagaatctg aatatgttca caggcacgcc gtacaacctt tgcttcactg
      661 gtgaagttat tttgcttcaa gtcagcttga agtgcttccg gtccgttatg caagataagc
      721 aaccctttgc cgataatgtg tagctgtttt gcgagccagc ggcgtgaacg gtgtaattga
      781 cgcgagagtg gaattccagg ggcatgcgga taaaaaatta ctgcgtcttt ttcgctgatg
      841 gcttgcggcc ggttgccttg cagtcctatg ttattgcgct ctaaccaatc cacaacccgg
      901 ctggctacgc caaacgctct ggaggcatcc tgggcgttgg agtaaagctt ggcatacagc
      961 agttgatgtt ctgaatgggc cgctccagtg gagtcgattt cgtatcggag tacatgccgt
     1021 tctttcgggc gatatcgtat cggcgtaatt aagggagttt gatctgcaat tccaagagat
     1081 gaaaatactc cagcaacata ggcaggtgta ccgaggcgaa ccagctgtgg aaacttcggg
     1141 tcgaagggcc aaagctgaag ccgcattccc ttttcaggca catctctcca caaggcagat
     1201 tggacgggca ttaagccaga gccttccgcc tcgctttgca gttgagacca ctggtctgca
     1261 ggagctgaat catccaggct attttgccag gtaaccgcga gatgaaccgg cgggctggac
     1321 gtctcgttcg catccaaaac cgggatcgtg aaatacgcag atagctttcg cgcgggtttg
     1381 aatttggcgc gagttaggtc gaatacgccg gcacgatagc ccggccgcag catagactga
     1441 acctcgtctc tcaacgtgcg gcgtgaggat tgtccactta atagatgccg gattcctgcg
     1501 gtgccagctt ttccggagag aatttcttcc tggttcattt ggggttcagg atcttataag
     1561 ccggaaggaa ttgctccagc acgaacatga tatcgtttaa ttcgcgcact ttgatcttcg
     1621 tccaggcatg tagaatgaga ataaagagtt caagcgcttc aaagagagcc acgcgctgcc
     1681 ggttcaccgg gccggtgtgg cgttcatatt cttctaaaaa tttctcgctg ataccaatca
     1741 gacgttcaaa gcgcgccact cgggttgcat ggtcgattgg ttctgaagcg acttccatat
     1801 catcgaattt gaccgtagcc atccctgcac gcaggaacgc gcagaggaat aaacccaggt
     1861 ctttggcagg ttctgccatg caggaagaat cgaagtcaat aaatccgatt tcttcaccgt
     1921 acatcagcac ttgcgccggg cggaatgtgc cgtgggaggg cactcgtggg tcaggcggtg
     1981 cgcttgcctc caggctgttc aatcgctcaa gcaatggcat tcctgccgcg gccaattgag
     2041 gcacagagac tgacaactcc tggattgatt cacgagtctg ctcttcgtca tcctcccagc
     2101 cgtaattcga tgtcaacttc gtgccagagt tgtgcaactc agccaatcct cgggctgtct
     2161 tacgcattgc ttccgtcaat tcatcgtagg actcagcggt tcctgcctta atggcactga
     2221 cggtcaattc cttcaatgta cgttcctgac gaatcggacc ctgaatcatg actttcaatt
     2281 ctgcatcgta agccagtggt tctgcgatct tcaccagcgt gctggacgac aaagcagtct
     2341 cccagaaagc ccgcatgctt tcataggcac tctgtccctt ttctttgcga taggttttag
     2401 cgacgaccag ttccggccag tgagtatgcg catcggcagg atagtcgagg ttatagacaa
     2461 tcgtgcagcg actcccgggt ttatagcgga caatcttagg atgggtcgca tcgatctgca
     2521 gctcctgata cgcaggagag cccgcccgaa tactgctcat caagtattcg cgtgactgct
     2581 cagggtcaat caacatatcc agagaggata gaactgcctc gggcttcttt gtccacagct
     2641 cgaggttcag cgctggaata taggcatgcc aatcttcact ccccagagca ttttccacgg
     2701 aaagcggacg ttccgagttg atacctgggg cggacagaac tccatctaaa tggatctcac
     2761 tcttatcaga ttcaccaggt ttgctgaccg ttaattcgta gaaacccttc cagttttccc
     2821 ctttgtagcg aacgttgctt gaatcacact cgtggaggat gagctgaccc gagcggaatt
     2881 cagggatgga ttcggagaag gctgcgcaga gtcgatccgg atctcggcca gccatcatcc
     2941 aatccggcag ttcccggaat atctggagtc cttccaggat cagagcgtct tgttcttgac
     3001 tgattggctg atctgttttt gccattcgat tctctcctcg agcaagggga tggtttgctt
     3061 tagacggctg cctttcattt tctcccaact atgtatggta agccggatga gactgatcaa
     3121 ttcatagatc gcgacgcgc
//
LOCUS       DASTJP010000776         7021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_687142, whole genome shotgun sequence.
ACCESSION   DASTJP010000776 DASTJP010000000
VERSION     DASTJP010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687142"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..2309)
                     /locus_tag="VFQ23_22750"
     CDS             complement(240..2309)
                     /locus_tag="VFQ23_22750"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HET9909484.1"
                     /translation="MISRRDFLKLSAAGTLSLYMTSRGKFLLKAQAQIPGGTLDPLSV
                     PKYVTPLLVPPVMPRAETIRLPGGKPADYYEISMKQISQQILPNGLPPTTVWGYGAVA
                     GSGRNPLLIHNAPSLTIEAQWKRPVRIKWINDLKDSNGNCRPHLLPVDSTLHWANPPQ
                     TPDVNGSSGTDRRPVFSETPGPYTGPVPIVTHVHGAVGVGDESDGYAEAWYLPLANDI
                     PSGFAAEGTWYNYFAGKAQAKHGVTWGPGFATFQYPNLNRASTLWYHDHALGMTRLNV
                     YAGPAGFFIIRGGPSGDQAVVDVRTAGPAILPGPAPREGDRFPSNKTYYEIPIAIQDR
                     AFNSDGSLFYPDTREFFDEFTGPFVPNSNVSPIWNPEFFGNMIMVNGNTWPFLNVEQR
                     RYRFRFLNGCQSRFLILNFGTIPGVEVWQIGNEGGFLAAPVNLTANHNNQLLMGLAER
                     ADLIVDFTNVPVGQHLLTNLGPDEPFGGGIADVDFEPSDPNSTGQIMAFQVAPALAPD
                     PTTPPLFLQLPALQPVPPATATRALALIEMMSNVHDGPSEARLGIVESGMAMHKMWSD
                     PISENPNVGDTEVWEFYNFTADAHPIHVHEVTFEVVNREALILDSEGEEPVQPVQLSG
                     VTRPPEAWESGVKDTVIAYPGEVTRIRTKFDTPGTFVWHCHIVEHEDNEMMRPYRIGP
                     VQPGQPA"
     gene            complement(2693..3157)
                     /locus_tag="VFQ23_22755"
     CDS             complement(2693..3157)
                     /locus_tag="VFQ23_22755"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9909485.1"
                     /translation="MKANLHSAVVKQLRRKLDPDEYKIIRQLWIDHSIAEDARDIPGL
                     MATLTEDCVYTVANNGVSWHGKAGATQFYQQLLTAFPDVHFDLQHIVIGPQGVFEEAH
                     VTGTYQSQWLDMPPPHGEPIQFDVILFFPWDPKQQLFTGERIYFFNVSLSSI"
     gene            3344..4216
                     /locus_tag="VFQ23_22760"
     CDS             3344..4216
                     /locus_tag="VFQ23_22760"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9909486.1"
                     /translation="MDITLALGGGGAKGNSHIGVIRRLEQEGFQIKAVAGTSFGGLVA
                     VFYALGYSPDEIEEMFAARDQTQLYTRPPTDGSSFLGLAGVLVFLEEVIGDRTFADLK
                     IPCVLTATDLNSGREVLLREGSLVDAISTTIALPGILPPRRIDGLVLVDGGTLDPVPV
                     VPARTLAPKLPVVAVVLTIEMGNPAQAWEIPLPENLPLRLIARVGKMRYGPALDVFLR
                     SFDIVTRAVTEYRLEVDKPDVIIRPQVTHIDTLDRVNVHEVVRKGEEAVDAALPQLKK
                     LFTWSKRIRRRIGL"
     gene            4219..4446
                     /locus_tag="VFQ23_22765"
     CDS             4219..4446
                     /locus_tag="VFQ23_22765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909487.1"
                     /translation="MTRDLRKYARQTNIRLAVGAVLVLLIVGVGLIYLIYGPGAAVTG
                     LLCLLAGISPIVLIVLSLAILDWINQRANRS"
     gene            4430..5089
                     /locus_tag="VFQ23_22770"
     CDS             4430..5089
                     /locus_tag="VFQ23_22770"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909488.1"
                     /translation="MQTVPETQLIQFEDWTLRVRESEDQSAKLLLLVHGLTGDENSMW
                     VFARDLPANYWIIAPRAPHTVDPGGYSWRPSQLGNLDTMRLDWLRQSAEALIGLVDTY
                     SASVGIDASVFDVMGFSQGAALCNVLTFLYPQRVRKAGILAGFVPNGLEELVSQRPLE
                     GKPFFVAHGTKDETVTVESARASIALLEKAGAHVTYCEDEVAHKVSMKCLRILKRFLT
                     D"
     gene            5209..6369
                     /locus_tag="VFQ23_22775"
     CDS             5209..6369
                     /locus_tag="VFQ23_22775"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9909489.1"
                     /translation="MNRNLSRRDFLKLAGAGLGALAFNPYRKLDLDYLAIPKRLPDFP
                     KSDIIGRTVDPGVDLRSSPTNDPTLNNSITKLGADTLVEWGRQVVGNVVGGLTNQRYI
                     ETPNGFIYSSVLQPTRNLPNTPLAEIPAGQPGFWAEVTVPYVDLAHEGVVASPWMQDH
                     ILYNFPPRLYYGQVVWIDQVRTTNGFAEYRWNEDANGHGYGYGASGEFFWGDGAAFKV
                     LAEEDVSPINPDIDPNDKRIEASLDYQTLSCFEGSTEVYFCRISSGLSNFIDPSTGQV
                     NDKMATPVGNLLTHWKIISLNMTAGTFQSGYSTPAVPWNTMISGDGVAIHGAFWHNAF
                     GEKRSHGCINVTPEDAKWIFRWTTPYISLAQSEMRVSLPDHGTIVLTSETRF"
     gene            6427..>7021
                     /locus_tag="VFQ23_22780"
     CDS             6427..>7021
                     /locus_tag="VFQ23_22780"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET9909490.1"
                     /translation="MDIANVTIGLAFLAGLASFLSPCVFSLVPAYVGYLGGRAVGGEA
                     TENNRLITFTHGLAFVLGFSIVFVTLGVATSAFGRLLFDLRFILSKVGGIIIIIFGLH
                     MIGVFRIPLLEYDTRVQKVPDRKWGYFSSALMGVFFSAGWAPCVGPVLGAILTLALNG
                     GSISTGVSLLSAYSAGLAIPFLLAALGIGWVSLTLKKY"
BASE COUNT         1663 a         1656 c         1825 g         1877 t
ORIGIN      
        1 aggtaagaac gatttagatc aatcatgaat caaaagcggc gcgatttcgc gccgcttttg
       61 taattttgat tattctattt caatgagatt ctgccctttg tgaagggcac catgatggtt
      121 gccaaatgac ttgaatcgaa aaaaagaata aaaaattggc gggatgacgg catgaacttg
      181 gcgacaaaat gacaaggacc gcctcgagcg gtccttgtca ttttgctgga tagtcttacc
      241 taagcaggtt gcccgggctg tacaggtccg atacgataag gtcgcatcat ctcgttgtcc
      301 tcatgttcaa cgatgtggca atgccataca aacgtgcccg gagtgtcgaa cttcgtccga
      361 atgcgggtaa cctcgcccgg atacgcgata accgtgtcct ttacgccgct ttcccaggcc
      421 tctggtggcc gcgttactcc gctcagttgg accggctgta ctggctcttc tccttcactg
      481 tcaagtatca acgcttcccg gttgacaact tcaaaagtta cctcgtgcac atgtatagga
      541 tgcgcatccg cagtgaagtt gtagaactcc cagacttctg tatcgccgac attcggattt
      601 tcgctgatcg gatcagacca catcttatgc attgccatgc cgctttccac aatgcccaac
      661 ctggcttccg aaggtccgtc gtgaacgttt gacatcattt cgatcagtgc cagagcccgt
      721 gtcgctgttg ctggtggaac tggctggaga gccggcaact gcaggaacaa cggcggagtc
      781 gttgggtcag gagccagggc aggggcaacc tggaacgcca taatctgccc ggtggagttt
      841 ggatcggaag gttcgaagtc tacatctgca atccctccac caaatggttc gtctggaccg
      901 aggttcgtaa gaagatgttg gcccactggc acattcgtaa agtcaacaat caagtccgca
      961 cgctccgcaa gacccattag caactgatta ttatgattgg cggtgagatt gacgggtgca
     1021 gccagaaacc caccctcgtt gccaatctgc cagacttcga caccagggat cgtgccgaaa
     1081 ttaaggatga ggaagcgcga ctgacatcca ttcaggaagc gaaagcgata gcggcgctgt
     1141 tcgacattca gaaacggcca ggtgttgccg tttaccatga tcatattgcc gaagaactca
     1201 ggattccaga tgggcgaaac attactattc ggaacaaagg gacctgtgaa ctcatcgaag
     1261 aactcgcgtg tgtctggata aaatagcgag ccatcagaat taaaggcgcg atcctggatc
     1321 gcaatcggaa tctcataata tgttttgttg gatggaaatc tgtctccctc ccggggcgca
     1381 ggacccggta gaatggcagg accggccgtt cgcacatcaa ccacggcctg atcacctgat
     1441 ggtccgccgc ggatgatgaa aaatcctgca ggaccggcat aaacattgag ccgtgtcata
     1501 cccagcgcat gatcgtgata ccaaagcgtt gatgcccggt taaggttggg atattggaat
     1561 gtggcaaagc ccggacccca cgtgacccca tgtttggcct gggctttgcc ggcaaagtag
     1621 ttataccatg tgccttcagc ggcaaaacct gaggggatgt catttgctaa aggcaggtac
     1681 catgcctcgg cgtacccatc ggattcatca ccaacaccta ctgcaccatg cacgtgcgtg
     1741 acaatgggaa ctggaccggt atagggcccc ggggtttcac taaagactgg tcggcggtca
     1801 gttccagaac ttccattgac atccggagtt tgcggcggat ttgcccaatg cagggttgag
     1861 tcaacaggta acaagtgcgg ccgacaattt ccattgctat ctttcagatc attgatccac
     1921 ttgatccgta caggtctttt ccattgtgct tcaatggtga gcgatggcgc gttgtgaatc
     1981 agcaacggat tcctccctga accggcaacg gcgccataac cccaaacggt cgtcggtgga
     2041 agcccgttag gaagaatttg ctgggagatc tgtttcatcg agatttcata gtaatccgcg
     2101 ggtttgccac caggtagccg aattgtttct gccctgggca ttacaggagg gaccagcagg
     2161 ggagtcacat attttgggac cgacaaaggg tccaacgtcc caccggggat ttgcgcctgc
     2221 gctttgagca ggaatttccc acgtgaagtc atataaagcg aaagcgttcc agctgcgctc
     2281 agtttcaaaa aatctcttct ggaaatcatg gttccctcca aactttacaa atccgaatct
     2341 gataagatgg gcaattagtt attgcaatga gagagaaata tggttggatg tttgatcggt
     2401 aacttctccc ttcttgtttg gaatggaagc aaactgaccc aataagctgt tatggctatt
     2461 gtctccgagt gaatttaccc ggacaagata gcgcgatcct gctaatcatg cgcattcata
     2521 acttgagtgc gcatgggtct attttgtttt gtatgtttat agtagtactt taggtgtaga
     2581 agaggtaagt gtcatttgtc acaaacttct gtgatttttg tcccaaactt tgagaaacca
     2641 ggccaggata ccccgtcggt gttatgagat ggccgtttga ttattttgcg agtcagattg
     2701 agcttaaact gacgttgaag aaatagatcc gttcaccggt gaacagctgt tgctttggat
     2761 cccacggaaa gaagagaatt acatcaaact gaattggctc gccatgaggt ggaggcatat
     2821 ccagccattg ggattgatag gtgcccgtga catgtgcttc ctcaaaaact ccttgtggac
     2881 cgatcacaat atgctgtaaa tcgaaatgga cgtcgggaaa agcagtcagc agttgctgat
     2941 aaaactgagt ggcacccgcc ttgccgtgcc agctaacgcc attatttgcc acggtataga
     3001 cacaatcttc tgtaagggta gccatcaaac ccggaatgtc gcgcgcgtct tcagcaatcg
     3061 agtgatcgat ccagagttgg cggatgattt tatattcatc aggatcaagt tttctacgga
     3121 gttgttttac gacggcagaa tgaagatttg ctttcatatt gtgttcatac gcctcattta
     3181 taattcttac gcttggttta tcgtttgtat tgtatgataa gccaatgaaa gtgataatat
     3241 gcagaagtag cctctacaga ttccgcgtac ggaatatttc tcctggttcc aacactatac
     3301 gactgttcta ataatttatc aacggctgag gaatttcttt gttatggata ttacgctcgc
     3361 gcttggtgga gggggtgcaa aaggaaactc acatattgga gtcattcgtc ggcttgaaca
     3421 ggaaggcttt caaatcaagg cagttgcagg gacaagtttt ggtggtctcg tggcagtctt
     3481 ttatgcactg gggtattcac ccgatgaaat cgaagagatg tttgccgcac gtgaccagac
     3541 tcaactctac actcgaccgc ctacggatgg ctcgtcattt cttgggctcg ctggtgtcct
     3601 cgttttctta gaagaggtga ttggagaccg taccttcgcc gatctgaaga taccctgcgt
     3661 tcttacggca acggatttga atagcggtcg ggaggtgctt cttagggaag gctccctggt
     3721 ggatgcgatt tcgacgacga tagccttgcc aggaattcta cctccgcgtc gcatcgatgg
     3781 tctcgtgctt gtggatggcg gcacattgga ccctgtccct gtggtgccag cgcgtaccct
     3841 cgcgccgaag ttgccagttg tggccgtcgt gttgaccatc gaaatgggca atcctgcaca
     3901 ggcctgggaa atcccgttgc ctgaaaacct gccgctgcgc cttatcgcaa gagtgggtaa
     3961 aatgaggtat ggtccggcac tggacgtttt tctgcgttcc ttcgatatcg tcacccgcgc
     4021 ggttaccgaa tatcgactgg aggtggataa accggatgtc attatccgtc ctcaggtgac
     4081 acatatcgat acgttggatc gagtcaatgt ccatgaagtt gttcgaaaag gtgaagaagc
     4141 agtggatgct gctttgcctc aattgaaaaa gttgtttacc tggagcaaac gcatccggcg
     4201 ccggatcggt ttataaatat gacacgtgac ctgcgaaaat atgcacgaca aacaaacatt
     4261 cgcctggcag tgggggcagt cttagttctg ctcatcgttg gtgtgggctt gatctatttg
     4321 atctatggtc ccggcgcggc agtaactggt ttgctttgtt tattagctgg tatctcgccg
     4381 attgtgttga tcgtattatc tcttgcgata ttagattgga taaatcaacg tgcaaaccgt
     4441 tcctgaaact caactgattc aatttgaaga ttggactctt cgtgttcggg aatcagaaga
     4501 tcagagtgcc aaattgttgt tattggttca tggactcacc ggcgatgaaa attcaatgtg
     4561 ggtcttcgcc agggatttac cagcaaatta ctggatcatc gctccacgcg caccgcacac
     4621 cgtggaccct ggcggctatt cctggcgtcc atctcagctc ggaaatttgg atacgatgcg
     4681 actcgattgg cttcggcaaa gcgctgaagc gttgatcggc ctcgtagaca cctattccgc
     4741 atcggttggg atagatgcga gtgtcttcga tgtgatgggg tttagccagg gcgcggcgct
     4801 gtgtaatgtg ctcacatttt tatatccgca gagagtccgc aaggcaggaa tccttgcggg
     4861 gtttgtcccc aatgggttag aagaactggt ctcacaacgt ccattggagg gtaagccatt
     4921 ttttgtcgct catggtacaa aggatgaaac tgtaacggtc gagagcgcgc gtgcctcgat
     4981 tgctttgctg gaaaaagcgg gtgcccacgt gacgtattgt gaagacgagg tcgcgcacaa
     5041 ggtgagcatg aaatgcctgc gcattttgaa gcgatttctt acagattgat taatccttac
     5101 taaccgaggc gtttactagc ctcttgttta caggtatcct tactccttaa gtagctttag
     5161 ccgttaagaa aagcgcctaa agtcgctact acattttctg gtgaattcat gaatcgtaat
     5221 ttatcccgtc gtgattttct caaattggca ggcgcaggcc taggtgcctt agcatttaat
     5281 ccttaccgga aacttgattt agactatctt gctattccca aaagactacc cgattttccc
     5341 aagagtgaca tcatcgggcg cacagtggac cccggtgtcg acctccggag cagcccaacg
     5401 aacgatccca ccctgaataa ttccatcaca aaattagggg cggacacact tgtggagtgg
     5461 gggcggcagg tcgttgggaa tgtagttggt ggtctaacga accaacgtta tatcgaaact
     5521 ccaaatggtt ttatctattc gtcggtgctc cagcccacac ggaaccttcc caatacgccg
     5581 cttgctgaga tacccgccgg gcaacctggt ttttgggcag aagtgacggt cccgtatgta
     5641 gacctggcac atgaaggtgt cgtggcctcc ccgtggatgc aggatcacat cctttataac
     5701 tttccccctc gtttatatta cggtcaggtc gtgtggatcg atcaggtccg tacgacaaat
     5761 ggttttgctg aatatcgttg gaatgaagat gccaacggac atggttatgg ctatggtgcc
     5821 tcgggagagt ttttctgggg ggatggagcg gcattcaagg ttctggcgga ggaagacgtc
     5881 tcgccgatca accccgatat tgatccaaat gacaaacgga ttgaagcgag cctggattat
     5941 caaacattat cctgttttga aggaagtacc gaagtatatt tttgccgcat ttcaagcggt
     6001 ctttcaaatt tcattgatcc ctccaccggg caagtcaacg acaaaatggc aactccagtg
     6061 ggaaacttat taacccattg gaaaatcata tcgttgaata tgactgcggg cacgtttcaa
     6121 tctggttatt ccacacctgc cgtgccgtgg aataccatga tcagcggtga cggggttgcg
     6181 attcatgggg cgttctggca taacgcgttt ggcgagaagc gctcgcatgg ttgcatcaac
     6241 gtaactcccg aagatgcaaa atggatcttc cgctggacga caccgtacat ctccctcgcc
     6301 caaagtgaga tgcgcgtctc actccccgat catggcacga ttgtgctaac gagcgaaaca
     6361 cggttttgat ttcgatctct cttgagaaca gtagcttaag aaattgattg ttttgaaagg
     6421 aatcgcatgg atatcgccaa tgtaactatc ggtcttgcat tcttagccgg actcgcatct
     6481 tttctttctc cgtgtgtatt ttctctagtt ccagcctacg ttggttacct gggtgggcga
     6541 gctgtgggtg gtgaggcaac cgaaaataat cgtttgatca cattcacaca tgggttggca
     6601 tttgtacttg ggtttagtat tgtgtttgtg acgctgggtg ttgccacctc tgcgtttgga
     6661 cgattgctct tcgacttgcg cttcattctc tccaaagttg gcgggatcat cattatcatc
     6721 ttcggtttac acatgatcgg tgtctttcgc attccactcc tggaatatga tacgcgcgtt
     6781 cagaaagtac ctgaccgaaa atggggatat ttctcgtcgg cgctgatggg cgtatttttc
     6841 tcagcaggct gggcaccttg tgtggggccg gtgttgggag ccattttgac attggcgctc
     6901 aatggaggtt ctatttccac cggtgtttcg ttgctttccg cctattcggc cggattggcc
     6961 atcccatttc tcctcgctgc tctgggaatt ggttgggttt cactcacctt aaagaaatac
     7021 g
//
LOCUS       DASTJP010000777         4847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1231307, whole genome shotgun sequence.
ACCESSION   DASTJP010000777 DASTJP010000000
VERSION     DASTJP010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231307"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /gene="bcp"
                     /locus_tag="VFQ23_22785"
     CDS             <1..471
                     /gene="bcp"
                     /locus_tag="VFQ23_22785"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HET9909491.1"
                     /translation="AVPLPAGIPAPDFNLLDDTNTPRKLSDFRGKNVVLYFYPADDTP
                     GCTKEACNFRDDYSAYEQAGVIILGVSPDTVASHVKFKKKFQLPFPLLADEDHKVCDL
                     YQVWGPKKFLGKEYEGVLRTTFLIDEQGNIAKVFEKVRPAEHSAEVLAAFGATS"
     gene            622..1635
                     /gene="coxB"
                     /locus_tag="VFQ23_22790"
     CDS             622..1635
                     /gene="coxB"
                     /locus_tag="VFQ23_22790"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET9909492.1"
                     /translation="MRHFVIVGILVLVVSVLTFFGVYAADLMPVAASMQAGYIDWMWN
                     IELIAMSFLFALIVVPMFYSFVVFRRKEGDTTDAEHVEGNTTLEIVWSIVPLFLVVIF
                     AYLGAVNLADTLREDPNAMVVKVTGIQWSWSFEYPPIDGVTVVSDELHIPVGKQVLLQ
                     MTSKDVIHSFWVPEFRVKQDLVPGRITELHITPIQEGDYKVRCAELCGTSHYSMEKPV
                     IVSTQEEFDAWLSEQKEFALELQATPEGAGQLLVAGNGCAACHSIDGAAGIGPTWFNL
                     YEHEVELSDGTVVIADDAYLFESIHEPQAKIVAGFETQLMPTYGFTDEEINNIVAYIK
                     TLR"
     gene            1651..3747
                     /locus_tag="VFQ23_22795"
     CDS             1651..3747
                     /locus_tag="VFQ23_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9909493.1"
                     /translation="MRQTNPVLKGLLLLVVGGALGYAIGLGIDLLVSGTLNFSGSVAS
                     AFAVLFAFTSFFFGVYGYRGITRGLVWQIVGMLLGAFFVTAIRALVGSEQIFGPFFFS
                     EPAWVFGGFTGVISFLFGVGSVDDWFKWARGIDTPEHKEDPPGWEKYFYVSYDHKVIG
                     IQYTITALFLIAVGGTFALIFRSELAFSQLQFLTTTFRLFNQTGPQFYNTIMSLHGIM
                     MIISILLGVSGIINYVIPLLLGARDMAFPRMNAFAYWVAVPATVLFLMSLVLGGFDTG
                     WTGYPPLSARAPVGMQMFFLAVFTAGWSSILGSLNVIATVLRLRAKGMTSMRLPIFVW
                     AGLATSIIALTATQLIGLSFQLVMFQRLFGMGFFDAAKGGNPVLFQHMFWFYSHPAVY
                     VFVLPGLGVISELLPVFVRKPLFGYRWIAMSSLGIAFVGFTVWAHHMFTSGMNEYLRV
                     PFMYSTLLVAVPTGVKFFSWVATIWGGKISAPTPMLFVIGAIIVFLIGGLTGPPNAAI
                     ALDLHLHDTYFIVGHFHDTIFGGFVFPFFAAIYYWFPKATGRRMNEFWGKIHFWLMTP
                     SFFVLTFGMMVVGLRGMRRRIVDYDPALGIDGAHLILTISAFLIAISVLIFFVNFFHS
                     IKNGEKAEGNLWNSRSPEWQVPSPMAAHNYEVPFEVVGEPYDYGLPGSKYVEFIADGV
                     RKHHHHHGEEATVHGS"
     gene            3737..4549
                     /locus_tag="VFQ23_22800"
     CDS             3737..4549
                     /locus_tag="VFQ23_22800"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9909494.1"
                     /translation="MDHKHGKSFLLSQGVFIFVYLAVLTALEFFIAVTFNAVALLLVV
                     ALVKAVLVVYYYMHVYKLNNENEGDEESYAYKTGTNRLGLWLFLISDSFVFGGLLLMR
                     INLLGLTRPELNQALGFFVTVVLLISSFFMNRGEAMMHNGDRKGFLNNTAITFILGLG
                     FLLGVVLIEWRTVPQIAEPVLALFGEHEGVEPLRPTSGVAGAAFFMMTGMHAFHVLTG
                     LILLAIVWINGRRGLYDEKQYPVEAAAVYWHFVDVVWIFFYPALYLVGVIAH"
BASE COUNT         1050 a         1097 c         1262 g         1438 t
ORIGIN      
        1 gccgtgcccc ttcctgcggg aatccctgcc ccagatttta atttacttga cgatacaaac
       61 acaccgcgta aactttctga ttttcgcgga aagaatgttg tattatattt ttacccggcg
      121 gatgatacac ctggttgcac caaagaggcc tgcaacttcc gtgatgatta ttctgcgtac
      181 gagcaagcgg gtgtgattat tttaggtgtg agccccgata ccgttgcgtc gcacgtgaaa
      241 ttcaagaaaa aatttcaatt accctttcct ttgctggcgg atgaagatca taaagtctgt
      301 gacctgtatc aggtgtgggg accgaaaaaa tttctgggga aggaatacga aggcgtttta
      361 cgaaccacgt ttctgatcga tgaacaggga aacatcgcaa aagtattcga gaaggtacgg
      421 ccggctgagc acagcgccga ggtgctcgcg gcgtttggtg caaccagcta accttgcgaa
      481 cggaatagat ccacgttgcc gctttttatg gtgaaaagtt actctgtgaa atttgtgaca
      541 agtgagcaaa agtttggtaa acttgtgtgg ttgtaaactg aggcactgct tgactgattt
      601 ttgtttcagg agaaacaatt tatgcgacat tttgtgattg tgggaatact ggtccttgtg
      661 gtgtctgttc tcacattttt tggcgtctac gcagctgatt taatgcctgt tgcggcaagc
      721 atgcaggcag gttatatcga ttggatgtgg aatattgagt tgatcgccat gtcctttctt
      781 tttgccttaa ttgtggtgcc aatgttttat agctttgttg tctttcgccg caaagaaggc
      841 gatacaacgg atgcagagca tgtagagggc aacacgactc tggaaatcgt atggtcgatc
      901 gttccacttt ttctcgtggt cattttcgcg tatctggggg ccgtaaattt ggctgatacg
      961 ctgcgcgaag atccgaatgc gatggttgtg aaagtaacag gcattcagtg gtcgtggagt
     1021 tttgaatatc caccgatcga tggtgtaacg gttgtctccg atgaattgca catccctgtt
     1081 gggaaacagg ttctgttgca gatgacttcc aaagatgtca tccactcatt ttgggtgccc
     1141 gaatttcgtg tcaagcagga ccttgtcccg ggtcgtatca cggaattaca tatcactcca
     1201 attcaggaag gcgattacaa ggtccgctgc gctgaattgt gcgggacatc ccattattcc
     1261 atggagaaac cggtcattgt ttcaacccag gaagaatttg atgcctggct gagtgaacag
     1321 aaggaatttg cgctggaact gcaggccaca ccggagggag ctggtcagct cttggtggca
     1381 ggaaatggat gtgcggcctg ccattcaatt gatggagcgg cagggatcgg tcccacctgg
     1441 tttaatttgt atgagcatga agtggaatta agtgatggca cggttgtaat tgcggatgat
     1501 gcctatctct tcgaatcgat ccatgagcca caggcaaaga tcgtcgctgg ttttgaaact
     1561 cagctgatgc cgacctatgg attcacggat gaagaaatta ataatatcgt cgcatatatc
     1621 aagacgttaa ggtaatcggt gaggaatacc atgagacaaa cgaatccagt tctaaaagga
     1681 cttttgcttc tcgtcgtggg aggagcactt gggtatgcga ttggcttagg gatcgattta
     1741 ttggtctcgg gcacgctaaa cttttccggg tcagtcgcat ctgcgtttgc ggtccttttt
     1801 gctttcactt ccttcttttt tggcgtatat ggttatcggg gaattacacg cgggttggtg
     1861 tggcagattg ttgggatgct gctcggcgcg ttcttcgtta cagcgattcg tgccctggtg
     1921 ggcagcgaac aaatctttgg cccatttttc ttcagtgaac cagcctgggt gtttggcggc
     1981 ttcaccggag ttatatcatt tctgtttggc gttggttcgg tcgatgattg gttcaagtgg
     2041 gcgcgtggta tcgatacccc tgagcataaa gaagacccgc cggggtggga aaaatatttt
     2101 tatgtttcct acgatcataa agtgatcggt attcaataca ccataactgc actttttctc
     2161 atcgccgtgg gtggtacatt tgcgttgatc ttccgttcag aattggcgtt ctctcaactg
     2221 cagttcctga caacgacctt ccgcctgttc aatcaaacgg gtcctcagtt ttacaacacc
     2281 attatgtcac tgcatggaat tatgatgatc atctccattt tgcttggagt atcaggcatt
     2341 atcaactatg taattccatt attgttgggc gctcgggata tggcttttcc gcgaatgaat
     2401 gccttcgcat actgggtggc tgtccccgcc accgtgcttt tcctgatgag cctcgttctc
     2461 ggcgggtttg acactggttg gaccggttac ccaccgctct ccgcacgtgc cccggtaggg
     2521 atgcagatgt tcttcctggc agtattcact gctggctggt cgtccatttt aggttcctta
     2581 aatgttattg cgaccgttct tcgtcttcgt gccaagggca tgacttccat gcggctgccc
     2641 atctttgtat gggccggtct tgctacgtcc attattgcgc tcactgccac acagttgatc
     2701 ggcctatcat tccaattggt gatgtttcaa cggttgtttg gcatgggctt tttcgatgca
     2761 gccaaaggcg ggaacccggt gctattccag cacatgttct ggttctattc acatccggcg
     2821 gtatatgtct ttgtgctccc gggtttgggt gtgatctctg aactcctacc agtattcgtt
     2881 cgcaagccat tgtttggcta ccgctggatt gcgatgtcct cactgggcat cgctttcgtg
     2941 gggttcactg tatgggcaca ccatatgttc acttctggca tgaacgagta tctgcgtgtg
     3001 ccgttcatgt atagtacgtt gctggtggcc gtgccaaccg gtgtgaaatt cttctcatgg
     3061 gtcgcaacca tctggggagg taagatatca gcacccacgc caatgctatt cgtcatcggc
     3121 gccatcattg tatttctaat tggtggtctc accggacctc caaatgctgc tattgccctg
     3181 gaccttcatt tgcatgatac ctactttatt gtggggcact ttcatgacac gatcttcgga
     3241 ggctttgtct ttccgttctt cgctgccatc tattactggt tccccaaggc gaccggccgt
     3301 cgcatgaacg agttctgggg caagatccac ttttggttga tgactccctc gttctttgtg
     3361 ctgaccttcg gcatgatggt cgttggtctg cgaggcatgc ggcgccgcat cgtggattat
     3421 gatcccgcgc ttggcatcga cggcgcgcat ttaatcctta cgatttcggc gttcctgatc
     3481 gctatttcgg tgctcatctt ctttgtcaat tttttccata gcatcaagaa cggcgaaaaa
     3541 gcagaaggta acttatggaa ctcacgctct ccagagtggc aggtcccatc gcccatggcc
     3601 gcgcacaatt acgaggttcc gttcgaagtt gtcggcgaac cgtatgatta tggtctgcct
     3661 ggttcgaagt acgttgaatt tatcgccgat ggagttagaa aacatcatca tcaccatggg
     3721 gaggaagcaa ccgtacatgg atcataaaca tggcaaatcg ttcctgttga gccagggcgt
     3781 cttcatcttc gtttacctcg ccgtattgac tgcacttgag ttcttcattg cggtaacttt
     3841 caatgcggtc gctttgctgt tggtagtagc gctggtcaag gcggtcttag tcgtctacta
     3901 ctacatgcac gtgtataagc tcaacaatga gaatgagggt gatgaagagt catatgccta
     3961 taaaacaggt acaaaccgac tcggactctg gctctttctc atttctgact cctttgtatt
     4021 cggtggattg ctgctcatgc gcatcaacct gctgggactc acccgacccg agctaaatca
     4081 ggcgctgggt ttctttgtga ccgtggtcct tctcatatca tccttcttca tgaaccgtgg
     4141 tgaagccatg atgcataatg gtgatcgcaa aggatttcta aataatacag ccatcacgtt
     4201 tatcctggga ttgggctttt tgttgggtgt ggtgcttatt gagtggcgca cagttccgca
     4261 gattgctgaa cctgttctag ctttatttgg cgaacatgag ggcgtagagc cgttgaggcc
     4321 tacctccgga gtcgcgggcg cggccttctt tatgatgaca ggtatgcacg cttttcatgt
     4381 gttgaccgga ttaattcttc tcgccatcgt gtggatcaac ggcaggcgcg gtttatacga
     4441 tgagaaacaa tacccggtcg aggctgcggc tgtatattgg cacttcgttg acgtcgtttg
     4501 gatatttttc tatccggcac tgtatctggt cggtgtaatc gcgcattagc ctcatgattt
     4561 tttggcgaac tcaccggtag cattttggcg tgaccgaaaa tcgaacacac aagtttagat
     4621 gataataaga cagatggcaa cacgttgggg aatctggtat aaacaaatac tgaagcataa
     4681 aatcggggcg gccaacaggt cgccccgatt ttcaagtcta aggaaccgct tggtgcatcg
     4741 agtgtgcaac agagtagaaa gaacatgaat agaagaatct ttttggtagg tttagcgtcg
     4801 ctcgtactga ttgctggtgt ggcaactgcg atattccttt tttcagg
//
LOCUS       DASTJP010000778         3244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1013656, whole genome shotgun sequence.
ACCESSION   DASTJP010000778 DASTJP010000000
VERSION     DASTJP010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013656"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VFQ23_22805"
     CDS             complement(<1..999)
                     /locus_tag="VFQ23_22805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HET9909495.1"
                     /translation="MHKHSHINCIVPPHMLKNIANNGTEAQKNVAISTLKTSEQMRGQ
                     RQALADFTAATFRVAAVGGKERIIYDAKNGSGLPGTIVRREGDPATTDVTVNEAYDGS
                     GVTYDLFSNIYQRNSIDGNGMRLDSTVHYRVGYDNAFWDGEQMVYGDGDEDMPADERL
                     FNRFTIAIDIIGHELTHGVTQFEAKLQYFQQSGALNESMSDVFGSLVKQYKTQQIASE
                     ADWIIGEGLFTPNVNGVGIRSMKAPGTAYDDQVLGKDPQPAHMRDYVNTISDNGGVHI
                     NSGIPNHAFYITALELGGFAWEKAGQIWYVTLKDKLNATSKFQDCANMTYQVAGELY"
     gene            1176..2396
                     /locus_tag="VFQ23_22810"
     CDS             1176..2396
                     /locus_tag="VFQ23_22810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909496.1"
                     /translation="MRLSVLLRGFFVLFLLTACANNQSLVTVTETPDISSITTETVSP
                     ATQTPDSKDEVNSLAGKIVFSAEGDVYVINADGSGRTRLTTNREEDFDPVWSPDGTQI
                     AFRSHRDGNEEVYVMNADGSNQLNISNAPRGDYSPAWSPDGVWIAFMSDRNGGNPNVW
                     VMEPDGSNPRQVTDMPGISEYPSWSPDSARIAFHCTFGRILSNGTGDFEICVVNVDGT
                     DLIQLTDTDGENKLPAWSPDGSKIAFQSNRDGWPTLPDYEPPGYDPREFGDDEIYLMN
                     ADGTDQTNITNNPREDEEFPAWSRDGHLVFSRYGCLIAITADGTGPTPIGSCEEGGHF
                     PDWYQPIKTAIQMSRQIAVVQLCLLTNATDRQISSALSLMVRLCISSQMIQHTKYGRP
                     GRLTERKLPTSAMM"
     gene            2306..3019
                     /locus_tag="VFQ23_22815"
     CDS             2306..3019
                     /locus_tag="VFQ23_22815"
                     /inference="COORDINATES: protein motif:HMM:NF027787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HET9909497.1"
                     /translation="MHQLTNDSAHEIWPAWSPDGTQIAYQRYDVDRSNPEIYRMSADG
                     FGQVNLTNNTGDDWNPAWSPDGAKIAFYSQRQEGRALYRMNSDGSAVTKIPGTDVGAS
                     PDWSPDGARLVYRQELPHNDEIFIINEDGTNPINLTNHPANDNSPDWSPDGQIILFES
                     IRDGNYELYTVETDGSDLQRLTDNDVDDQHARWSPDGSTILFTHHRELYLINPDGSNQ
                     RPLGGKAISGIFAAWGPCN"
BASE COUNT          800 a          759 c          796 g          889 t
ORIGIN      
        1 atataattct ccggccacct ggtaggtcat atttgcgcag tcctggaatt ttgaagtcgc
       61 gttcaacttg tctttcaatg ttacatacca aatctgacct gctttttccc aggcaaagcc
      121 gccgagttcc agtgcagtga tgtagaaagc atggttgggg attcccgagt tgatatgaac
      181 gccgccgttg tcactaattg tattgacgta atctctcatg tgagctggct gcggatcttt
      241 ccccagcacc tgatcatcat acgcagttcc aggtgctttc atcgagcgga tgcccacccc
      301 gttcacattg ggcgtgaata ggccctcacc gatgatccaa tccgcttcgg atgcaatttg
      361 ctgggtttta tattgtttga ccagtgaacc gaagacgtca gacatcgatt cgttcaatgc
      421 accagattgt tggaaatatt gcagcttggc ttcgaactgg gtcacgccgt gtgttaattc
      481 gtggccgata atgtcaattg caatggtaaa gcggttgaag agacgttcat cggcaggcat
      541 gtcttcatca ccatcaccgt agaccatttg ctcgccatcc caaaatgcat tgtcatagcc
      601 aaccctgtaa tggaccgtcg agtccagacg catcccattg ccgtcgatcg aatttctttg
      661 ataaatgtta ctaaataaat catacgtcac gccactgcca tcataggctt cgttgactgt
      721 tacgtcggta gtggctggat ctccctctct gcggactatc gtaccgggaa gccctgaacc
      781 attcttcgca tcatagatga ttcgttcttt accgccaact gctgcgacgc ggaatgtcgc
      841 ggcagtaaaa tctgcaaggg cttgtcgctg gccgcgcatt tgctctgacg tcttaagggt
      901 gctgatcgca acattctttt gagcttctgt gccgttattg gcgatgtttt tcagcatgtg
      961 cggcggaacg atgcaattaa tatgggaatg cttgtgcatg aggattcctt tcctgttgtg
     1021 tagatagtaa caagccttgt cagtatacgt ctttttcagg gaaatttcaa tgtagaggtt
     1081 agagagcttg cgccttgtat gattcttttt taggaacttt tcttttgcat ccggtgtata
     1141 tcactaaagt caaatgatat gctaaggaga gcttaatgcg cttatctgtc cttttgagag
     1201 gattttttgt tttgtttttg ttgacggctt gtgcgaataa tcagtctctt gtgacagtca
     1261 ctgaaactcc tgatatttca tcaatcacca ctgaaacggt atcccctgcc acacaaacac
     1321 cagattccaa agatgaagtc aactcgctcg cgggaaaaat cgtcttttct gcggaaggtg
     1381 atgtttatgt gattaacgcc gatggatccg ggagaacgcg attaaccact aacagggaag
     1441 aagatttcga tccggtttgg tcaccagatg ggacccaaat cgcctttcgt tcccatcgtg
     1501 atggaaatga agaagtatat gtgatgaatg ctgatggttc caatcaactt aatatttcga
     1561 atgcgcccag aggagactat tcaccagcct ggtcacccga tggagtttgg attgccttta
     1621 tgtctgaccg aaacggaggg aaccccaacg tctgggtgat ggagcccgat ggttcaaacc
     1681 ctcgtcaggt gacggatatg cctggcatat cggaatatcc cagctggtct ccggacagcg
     1741 ctcgcatcgc ctttcactgt acttttggcc gaatcctttc caacggtaca ggcgactttg
     1801 aaatctgcgt ggtcaatgtg gatggaactg acttgatcca actgacggat acagatggcg
     1861 aaaacaagct gccggcctgg tcgccggatg gaagcaagat tgcctttcaa agcaaccgag
     1921 atggttggcc cacgctgcct gactatgaac ctcctgggta tgacccgcgg gaatttggcg
     1981 acgatgagat ttatcttatg aacgctgatg ggacggatca gacgaacata acgaataatc
     2041 cgcgcgaaga tgaggagttc ccggcctggt ctcgtgatgg tcacctggtg ttctcgcgtt
     2101 atggatgttt gatagcgatc actgctgatg gaactggacc taccccaatc ggctcatgcg
     2161 aagagggagg tcatttccct gattggtatc agcccataaa aacagcaata cagatgtcaa
     2221 gacaaatagc agttgttcaa ttgtgtttgt tgacgaacgc aacggacaga cagatatctt
     2281 cagcgctctc gctgatggtt cggctttgca tcagctcaca aatgattcag cacacgaaat
     2341 atggccggcc tggtcgcctg acggaacgca aattgcctac cagcgctatg atgtagaccg
     2401 ttccaaccct gaaatttaca ggatgtcggc tgatgggttt gggcaagtga atctgacgaa
     2461 taataccggc gacgattgga atccggcctg gtcacctgat ggggcgaaaa tcgcctttta
     2521 ttctcaacgt caagaaggta gagcgcttta ccggatgaat tccgatggtt cagcggtgac
     2581 aaaaattcct ggtacagatg ttggggcctc acccgattgg tcacccgatg gagcccggct
     2641 cgtgtatcgg caggaactcc cgcacaacga tgaaatcttt attattaatg aggatggaac
     2701 gaatcccatc aacctgacga atcaccctgc gaacgacaat agtccggatt ggtcgccgga
     2761 tggtcagatc atattgtttg aatcaattcg ggatggcaac tacgagttat acaccgttga
     2821 aacggatggc agtgatttgc aacgcctcac cgataacgat gtggatgatc aacacgcccg
     2881 ctggtcacca gatggttcaa ctattctatt tacccatcac agagagttat atttaataaa
     2941 ccctgatggt tcgaatcaac gaccgcttgg agggaaggca atatctggca tatttgcagc
     3001 ctggggacca tgtaattaat ctgcaagtct atgtgggtgc cttgagaact tctgccaaac
     3061 ttgggaggac ttagatatct gctgcttgga cagataattc ttcagctttg acatttaagt
     3121 cgttcagcgc agcaaacgtt aattgtgtgc ggtcgagttc tgttcttatc tgacacaatc
     3181 cttcaaatag cgcgttataa cttttaccga tagttgtttc ccatttcaga ttcagcatcc
     3241 tggc
//
LOCUS       DASTJP010000779         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_965827, whole genome shotgun sequence.
ACCESSION   DASTJP010000779 DASTJP010000000
VERSION     DASTJP010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965827"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..1414
                     /locus_tag="VFQ23_22820"
     CDS             284..1414
                     /locus_tag="VFQ23_22820"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET9909498.1"
                     /translation="MQALQRIILPPRYENRVLDVHAKFLHISLWFTFFIAMYFASINT
                     ALTRLAFFVLALSSLVGLILNYHQKYILSASIPVIMGTIALFFNFFDGISLYDPGIVA
                     IPLLIILTSFLFGSRLIYRSAFVNILGVGLLVYFERAGIISPPYLASNERFIIISILV
                     VFTAILQKRIIQSWEQAVEVSHESEHRVREAYVLTLEGWAKTLEFHDRETLGHSQRVT
                     SLCQRLAEKLGIVDPQELEYIRWGALLHDIGKLAIPYDILRKQGELSNEEWEIIKSHP
                     ALAEELLGNIEYLKPALSIPRAHHENWDGTGYPRKLIGEEIPFHARLFTVVDNWDALT
                     TDRSYRKAWSREKTINYMREQSGKRFDPKIVDVFITHIALAE"
     gene            1573..1827
                     /locus_tag="VFQ23_22825"
     CDS             1573..1827
                     /locus_tag="VFQ23_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909499.1"
                     /translation="MAEKYTPLESYLRALHPSQEEVTLTFEIIEGILKESLPAAAYQD
                     GAWWGNQKQGTQVEVIAWMDAGWMVEIVNLNEKWVRFVRQ"
     gene            2073..2564
                     /locus_tag="VFQ23_22830"
     CDS             2073..2564
                     /locus_tag="VFQ23_22830"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET9909500.1"
                     /translation="MKIQEGDTVMHWTHGLGKVVRLEERTLAGEVVLYYAIQIGDMMV
                     WVPADDMLETRLRLPTDAAEFQSLMGNVSEPGEPLPENRRERKTLLVEWLKDGRTESR
                     IRVIRSLVTYRQVRPLNADEQAILKRSKNALLEEWSCALSSPVPQAETELHRLLKVQT
                     LEG"
BASE COUNT          671 a          621 c          692 g          645 t
ORIGIN      
        1 tgtggcaaga gaagttaact ccaattcaaa ttgtttagcg tgacactgtt ttgccaaagg
       61 gctaacagcg aggcgaaggt cacaatgcaa gtgcttgact gcgcgctgta ggcctattcc
      121 aagaagaatc aatacctgtg cgtcgatgtg aaggttctat tcagggcatt acggaccgcg
      181 actacgtatt tatgcgacga aagcgggtga tatacttcaa atcaaatttt ctgtaaataa
      241 cgttcaatcc gtgtagttga tttgaagaac gaggagttgt cacatgcagg cgcttcaacg
      301 gattattctt ccgcctcggt atgagaatag ggttttggat gttcatgcga aattcctgca
      361 catttccttg tggtttacat tctttatcgc gatgtacttc gcctcgatca acaccgcctt
      421 aaccaggctg gcattcttcg tcctcgcgct gtccagtctc gttggtctga ttctcaacta
      481 ccatcagaaa tatatcctga gcgcctccat ccctgtgatc atggggacca tcgcattatt
      541 cttcaatttc tttgacggca tttctctata tgacccgggc attgttgcga tccccttgct
      601 catcatcctg accagcttcc tgttcgggag ccggctcatt tacagatcag ccttcgtgaa
      661 catcctgggg gttgggctgt tggtgtactt cgaacgcgct ggaatcattt cccctccata
      721 ccttgccagc aatgagcggt tcattatcat ttccattttg gttgtattta cagccatcct
      781 tcagaaaagg atcattcaaa gctgggagca ggcggttgag gtctcgcacg agtctgaaca
      841 cagggtccgg gaggcatacg ttctcaccct ggaaggctgg gcaaagaccc tggaatttca
      901 tgatcgggaa acgctgggtc acagccagcg ggtcacctcc ttgtgccagc gtctggctga
      961 aaaactcgga atcgttgacc cacaggaact tgagtatatt cgctggggcg cgctccttca
     1021 cgatatcggg aaactggcaa ttccctatga catcctgcgt aaacaaggcg aactgagtaa
     1081 cgaagagtgg gaaatcatca aaagccatcc tgcactcgcc gaagagctgc tcggtaatat
     1141 cgaatacctc aagccagccc tgtcgattcc acgcgctcat catgaaaact gggatggaac
     1201 aggctatccc cgcaaattga taggagagga gattcctttt catgcccggc tctttaccgt
     1261 ggtagataat tgggatgcac tgaccacaga ccggtcctat cgaaaagcat ggtcccgcga
     1321 gaaaaccatc aactatatgc gtgagcaaag cgggaaacga ttcgacccaa aaatcgtgga
     1381 cgtgtttatc acccatatcg cgctggcaga gtaggagatc ttgcaattat taaattgacg
     1441 cccatactat gagcggaacc gcacctgaaa tcacatataa gatttttacc ttcaaagttg
     1501 taacttattc gatgggtgaa ttgcttgcgt aataagagtt gctgaataca attgagtgat
     1561 aagaaagtat gcatggctga aaaatacacc ccacttgaat catatttacg agcgcttcat
     1621 cccagtcagg aagaagtcac actcacgttc gagatcatcg aagggatctt gaaggagagc
     1681 ctgccagccg cggcctatca ggatggcgca tggtggggaa accagaagca aggcactcag
     1741 gtggaagtga tcgcctggat ggacgccggc tggatggtgg aaattgtgaa cctgaacgag
     1801 aagtgggtcc gttttgtacg tcagtagaca ataggagcag acagagactc ttcgcggagt
     1861 ttaccctgag ctgaagagag attcttcgct tcgctcagaa tgacagacga agggctcaga
     1921 gtgacacgtt ctccattcag gaagcgtcag tgttggacgc agggttcgtg atacaatcag
     1981 tttaaatccc tgaattgacg ggttcgaaat gccagtctat ttcttcgact ggcgtttttc
     2041 tttttttccc atagtacgaa acggagatac ttatgaagat ccaggaaggc gacacggtca
     2101 tgcactggac gcatggactg gggaaagttg ttcggttgga ggagcggacc ttggcaggtg
     2161 aggtcgtcct ttactatgca atccaaattg gcgatatgat ggtctgggtt ccggcagatg
     2221 acatgctgga aacgcgcttg cggctcccga ccgatgccgc tgagtttcaa agcttgatgg
     2281 gcaatgtgtc cgagccgggg gagccgcttc cagagaaccg tcgtgagcga aaaactctgc
     2341 tggtggaatg gctgaaggat ggtcggacgg aatcacgcat tcgtgtgatc cgcagtctgg
     2401 tcacgtaccg gcaagtccgc cctctgaatg cagacgagca agccatcttg aagcggtcga
     2461 aaaacgcgct gcttgaggag tggagttgcg cgctgtctag tcccgtgccg caagctgaaa
     2521 ccgaactaca ccgtttactc aaagtccaga cgctggaagg ttgagtatga gcgagaggaa
     2581 ataaaccatg tccatcaaca cgaaggtact tgtacggaat gccagagag
//
LOCUS       DASTJP010000780        21039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_914960, whole genome shotgun sequence.
ACCESSION   DASTJP010000780 DASTJP010000000
VERSION     DASTJP010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 21039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21039
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914960"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..820
                     /locus_tag="VFQ23_22835"
     CDS             83..820
                     /locus_tag="VFQ23_22835"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909501.1"
                     /translation="MKFKTILGAMFVLALIMTACAPAETASPTAMTEPTTESTSTEPA
                     MTESPAMTESPAVTDTQAVAETPTVSVPVTGEVTVEVSETTDYGPILVDGNGMSLYVF
                     IADTQDSGTSTCGDDDGCATEWPPLLCSAEMSLGNDNANTNSNTNDNSNSNTNSNANS
                     NDNTNTSGTPGADDCASETIAGEGVDALLLGTITRDDGSTQVTYNGWPLYYFAADTTP
                     GDMNGQGIDEFGGLWFLVSPTGEAIEQ"
     gene            996..2258
                     /locus_tag="VFQ23_22840"
     CDS             996..2258
                     /locus_tag="VFQ23_22840"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9909502.1"
                     /translation="MSVSNSTSAKKTSRLTAPILVNSTEVEIMRALRRQGRISRTEIS
                     NITGWSKAKASQEIRRLVNKGYLIEIGEGASQGGRRPRLLRINNQLGFIVGIDIGATS
                     LDVALADVSGLILKRRSEPTDVKLSPVSVLGRCSELLLELIQDQSVNPHQILGIGIGV
                     PGPVDFARGVLVAPPLMPEWENFLIRDFFKKTFTSAFVVVDNDVNIMALGEQRSGDAE
                     NVDHFLVVKIGTGIGCGIMASRKIHRGSSGSAGDIGHICVDTTGPVCRCGNRGCLEAM
                     AAGPAISEKATQAARERKSKLLLKMMQNNGNVLTPEDVNMACREGDETALEIIRSSGR
                     MIGEVLAGLVNFFNPSQIYITGGISNFGNHLLIAIKRAVLRRSLPLATSGLDIVFSRM
                     GSDAGVLGAIMLALEYLFIVENDLHFSF"
     gene            2423..3478
                     /locus_tag="VFQ23_22845"
     CDS             2423..3478
                     /locus_tag="VFQ23_22845"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9909503.1"
                     /translation="MKQSKYLTLFSLLIVASVVLTACGGAAATATPAPQQDGFVIGVS
                     NTLVGNGWREQMICSIKAEATASGLVDKVVVVNRNGGPTEQIADLEGLISQGVDAIII
                     NPTDREGLNAVIETAIAQGIVVVAVDQAVTAEGAYVVTNDQTAYAQLGAEWLFEQMGG
                     SGNVVEMRGIDGVPADTDRHNGFLAALEKYPDINVVAEVFTGWDPSVGAQQALDLITT
                     TQVDGIWTSGIDYTVVEQFIAANAPYVPIVGADNNGFIKQLIELEGDGLTGAAVTNPP
                     AIGAVGTSIALQALTGKNPEHVVTLTPKVYATGDSALNDLFAPDEQPGWSTFVNIDPY
                     TSYSGSADVSACKGPGE"
     gene            3610..5091
                     /locus_tag="VFQ23_22850"
     CDS             3610..5091
                     /locus_tag="VFQ23_22850"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET9909504.1"
                     /translation="MTEKINPYLLETKGIAKSYGSVVALTSVDLVVRHGEIHALLGAN
                     GAGKSTLVKILAGVQSADAGELQVNGKSVTFHSPADAMAAGISTVFQDPSLIPDLTIE
                     QNLRLSNIEESKFREWLDWFNLGQLDLSALVRELPLETLRIADLARAVARKPQLLLLD
                     ELTAALTADQAERVFKLLAHWKEIGNSAVLITHRLAEVMSICDRATILRNGHNVDVLE
                     TSKVNEHQLVAAMLGQLAEAGSEETKGDVDRNQGDPAFTVMELSSGDVVRDVSFSVKS
                     GEILGIVALEGQGQDRLFELLSGDRQPSSGKILVHGIAKHFRSPFDAVQSGVVLVPGD
                     RLRALLPDLSIRQNLAIPLFNRIREWFGLPRDENEKVAGAINQLSIDTRAASQVRRLS
                     GGNQQKVVIGRWLVSGFETLLCFDPTRGIDIQTKREIYILLRRLASEGSAVLLYTSEL
                     AEIPLVCDRVLVMHAGKIVAEQDAATATESSMLTAAHGLDVTK"
     gene            5088..6092
                     /locus_tag="VFQ23_22855"
     CDS             5088..6092
                     /locus_tag="VFQ23_22855"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909505.1"
                     /translation="MNKVHRTGNNNLWQKILSRNRWVLGAWLLLGVLLLWYSTLIPVF
                     GEFQITSITKNSLPLVYLAIGQAIIVIGGGIDLSLGSLLLLGNVIAARFMEDQSPGVA
                     FVIGFAMILGIAVLNGITGYVISVSRIPDIVVTLATSYIWSGMALLILSSPGGGTAPE
                     VRWLFSGSESGIGGSPIMPILMLIIPTLLVFTYLNRSQNGLSIYAIGSDKTAAQLAGL
                     DVRKAKVLSYAIGGAMASLAGLATVALTGTGDPRFSIGAIATLNSVAAVVLGGVALTG
                     GIGSVIGMVPAAVILIMLNPILSAMGFDPNSAQVVQGILIAAVMMVGGFVTLRRERAA
                     "
     gene            6089..7117
                     /locus_tag="VFQ23_22860"
     CDS             6089..7117
                     /locus_tag="VFQ23_22860"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909506.1"
                     /translation="MTNKTNSSEPGMLTADSKTEVKRAGFISRVRTIAEERPALLLVG
                     VLFILVVVTGIIQPNYISVAGIRNTLLLAAPLGILAGAQTILMLTGGIDLSVTMIATG
                     SAYVAANQSPNGALIAIVFGILVGVIAGTANGIGITVFRVNPLIMTLAMSGILLGLFT
                     GWTLTILQGATTVADFIKLMGTGSFFGNRIPYSVVIWIVVSILLIWLLERTGWGRLIY
                     AIGNNEGAVRLAGIRVWQVRITAYMVAGVLGSISGILLAGRTGAVDLQLANAFLLPSV
                     AAAVIGGTSIFGGIGKYTGTILGALILSVLNSMLTFLNAGQAVQHIVYGVIVLALAWG
                     YASLTRRT"
     gene            7139..8332
                     /locus_tag="VFQ23_22865"
     CDS             7139..8332
                     /locus_tag="VFQ23_22865"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9909507.1"
                     /translation="MMHKVTMLGTGLIGMFYTKTLHRNRSLDQVAVVYSRTEERAKQF
                     ASEWKIPKYTTDLEAAINDPDTDVVVIGLPNNLHEEAVRLATQAGKHVLCTKPLGRNA
                     EEARRMLKMVEEAGVFGGYLEDLAYTPKTLKAMQSVRNGAIGKVLWGRSRETHSGPHS
                     DWFWNKEISGGGAIVDLGCHCIEIIRNFIGKDVRPVEVMCWADTQVHPIDAEDSAIGL
                     VKYASGAIGQFEVSWAFRGGMDLRDEISGTKGTIWLNHWMRTGFELFTAEGEKDYVSE
                     KTESETGWLFPVGDEIGGLGYITMFDDMLDSIDGGKQPMESFYDGYVVNAIMDAAYRS
                     AASKQWEPVLIEDWRGSDDAETEVQMAEYDADHFLIKQEKMPDGKTKYILKQKSSGAI
                     IQKVM"
     gene            8360..9106
                     /gene="deoC"
                     /locus_tag="VFQ23_22870"
     CDS             8360..9106
                     /gene="deoC"
                     /locus_tag="VFQ23_22870"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET9909508.1"
                     /translation="MSNLNSKTITYEQLAKVIDHSLLRPELTAADVLAGCELAARYHV
                     ATVCVKPCDVKLAKEALKDSDVLVSTVVGFPHGSNLTDVKVAEAEKAMDDGALELDMV
                     LNIGGLRSGKHDFVREDIKAVCDAAHARGVKVKVIFENAYLTDEEKITACKLSEAAGA
                     DWVKTSTGFASGGATLEDLRLMRANVSEKVQIKSAGGVRTLQSLLDVIDAGVTRSGAT
                     ATATILDDFKKMKEGTPLTTPDPDSVSGTY"
     gene            9159..10325
                     /locus_tag="VFQ23_22875"
     CDS             9159..10325
                     /locus_tag="VFQ23_22875"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9909509.1"
                     /translation="MAKQLTKQTIGVGVAGTGFIGPAHVEGLRRNGIRVLGLVGSTGE
                     KAEQKAAELGIPHAYASLDEMLADPEIDVVHLATPNHLHHPHARAALLAGKHVVCEKP
                     LAMTSEESADLVQLAVEKQLVNAVNFNIRMYPLVHQARSMVQSGELGDLFILQGSYLQ
                     DWLLFPTDWNWRLETNLGGTLRAVGDIGSHWLDLLTFITGLRVEEVYSDFKTFHPVRK
                     KPAKPLETFTGKILTPEDYIDQPISTEDYATILLHYENGVTGVVTVSQVSSGRKNRLF
                     YEINGSKSSLGWDSERPNELWIGRRTEPNQTLLKDPSLLSPEARAVTSYPGGHNEGFP
                     DTFKQLYAKVYDYIISGDYTKTPDFPTFADGHYEMQLCEAIERSAREKTWVKVK"
     gene            10338..11252
                     /locus_tag="VFQ23_22880"
     CDS             10338..11252
                     /locus_tag="VFQ23_22880"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET9909510.1"
                     /translation="MKLGFLTAALPGNTLEQAAKWGAESGFEAIEIACWPPGKATRRY
                     AGVSHIDVNELDEAKAREIRTMLDGYGLTISSLAYYPNPLHPELEHREMVINHLKKVI
                     EAAELLEVPIVGTFIGKDKNKTVPQNLEDYAKVWPPIVKFAKDHGVKIAIENCPMLFS
                     YDEWPGGNNLAASPAIWRQMWEIIPDDNFGLNLDPSHLILQMIDYERVIKEFSDKLFH
                     VHAKDLHIDRDGLYNNGVLAQGMGWQIPRLPGLGDVDWAKFIGTLKSVGYDYVVSIEH
                     EDRNYEGDEELVKKGFYLSRDVLKPLIQ"
     gene            complement(11329..12174)
                     /locus_tag="VFQ23_22885"
     CDS             complement(11329..12174)
                     /locus_tag="VFQ23_22885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909511.1"
                     /translation="MVLASFVVLAYVAAAAILSWLYFRRYVMTRPPIGVFNLGDIAVM
                     IGAIILVPFLYLLVPIWILASLLALASISILYFTWEPVFRSVWSIWLVTILLAGSDLA
                     TALLPGIPSIWFFAVNNIVLVISVIGVTNLWAQSGMKARHAAILGAALMVYDLIATSL
                     LPLMNDLFIRLEGLPFAPEIAWLVNAEGDWLSIGLGDLVMAAVFPLVMRKAFGRTVGM
                     IALVIGLGAITLLILSLISGLLVGTFPVMVVLGPLMVLQYVYWQRQLKQERTTWQYLQ
                     AEPLK"
     gene            complement(12184..13635)
                     /locus_tag="VFQ23_22890"
     CDS             complement(12184..13635)
                     /locus_tag="VFQ23_22890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9909512.1"
                     /translation="MFETAFAQLRYAASVLFAIPFDLRALDRLVEGILATQHEFGTLG
                     TDSAEFLGGPELDEESRREIQLRRFRTQALRAVNETDYYKQQFDQLGIDPANLKYEDT
                     PRIPITPKEALRSDPEAFVRRGSQPAFRTTTTGTTGKPTSVYFTSHEIQATGLLTAIT
                     FLQSGQITPEDIVQISTSSRATLGNTCFSQACQRIGAVWYQTGLVDPTHALMLLSEEH
                     HLPGKKSRASFLNTYASYLGELVEYGLSNRYSPADFGLERISVGGEIVTEGLKKRCQK
                     LFGSVEFIQGYAMTETWPFSGTRCAESHLHFEPSQGLMEVLDPDTHEPVKLGEFGTIV
                     ATPFPPYREASVVLRYDTEDVVQAINGPLTCNLKHWPATSDLQGKLRLSVRGPDGWAH
                     PRQVREALEAIEEVPLPARCGFWSVPGGVAVEVLVRKAESSIHKKIEQNLEEHSVPIK
                     ELHLVENPSQLKHPLPLRCDLKEALFTPVRRLR"
     gene            complement(14216..17164)
                     /locus_tag="VFQ23_22895"
     CDS             complement(14216..17164)
                     /locus_tag="VFQ23_22895"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9909513.1"
                     /translation="MTSNPSGTVTFLFTDIENSTRLAREYPDSWDDARARHHAILREA
                     IELNNGFVFQVIGDACCAAFHKAGDALKAAVRAQQDLQNEPWGEATIYVRIGIHTGEA
                     EFNGNDYRGYTTLSFVQRLMSAGHGGQILVSSVTEQLLREQLPEQIGLGNMGMQKFAG
                     MASPVRVFQVIASDLPTEFPPLRTLDNLPNNLPTQLTSFVGREKELADVKRLLQNTHL
                     LTLIGLGGTGKTRLSLEAAGEMLHLYPDGVWFVELAPILDPRLVPRTTAIAIGLRDEP
                     QRPVIDMLCDYLHEKKILIVLDNCEHLVDACARTADRILRAAPDTRILASSREALGIG
                     GEVTYRVPSLDLPDLSHLPPVDSLSQYEAVKLFIDRATSAVPTFTVTNDNAPALAQVC
                     YHLDGVPLAIELAAAKIRVLTVEQIAKRLDDRFRLLTGGSRTVLERHQTLRAAIDWSF
                     NLLLEDEQALFQRLSVFVGGWTLEAAESVCEGGSVKREDILNRLEQLVNKSLVITEEV
                     EHESRYHMLETIRQYAREKLFDTGQDKTLRQKHGDWFLKLAEDAERGLLFGKEFAKWM
                     DRLEHDLDNFRAALAWLLETEQIEKCVRLASKLGLFWIDRNFYSEGRYWLETGLKYRD
                     VLSKNTLAKAIRISGRLLARMGEYDQAIRHGEESVALFREISDKFDVALAIQFLGETI
                     YERGDDNSDTQYYEEALWLYRELGLREFANGMMIELGWHHVSVGNFLDGFSTLEECLQ
                     TARELNELPSIAYSLFVLGICRWRYAELDKSEAALKEGLRLYKQLGDRWFTHACLIGL
                     AGVASLRGKGEQAAKLIGFSDKGMEFIGGVKPPFWMRDYENPIIDSIRSQMEDSAYTK
                     AWNEGYSMTLEQAIELAESQFVLSVPMSELKPEIGASILPSQREVEKQKYGGLTERER
                     EVAAQIAQGKSNQTIARELFVGLKTVEAHVTRILSKLGFTSRAQIAGWAVAKGLAEAP
                     SDLDTLARKE"
     gene            17306..17701
                     /locus_tag="VFQ23_22900"
     CDS             17306..17701
                     /locus_tag="VFQ23_22900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909514.1"
                     /translation="MASEELQPHLFTGRWIGETQGYVMPAHIWEITQHALGFLMVSTR
                     WEGQTEVAGFSAKVVPNELAFIISGSKTFKATLVDKQHFVIPGWCTNDVRGGIGPAYD
                     VVFSRPGIAELTGRQAYAQYLEHAVQEKN"
     gene            complement(17698..18855)
                     /locus_tag="VFQ23_22905"
     CDS             complement(17698..18855)
                     /locus_tag="VFQ23_22905"
                     /inference="COORDINATES: protein motif:HMM:NF024601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HET9909515.1"
                     /translation="MDTTFDLDSILSVLRTRLCSRTPFLGSLLLFAETISVDTIPTAA
                     TNGRQIMINPQFFERLTLDQQEAVLLHEVLHAALLHVSRGTGRDAKRWNIAADIVVNG
                     ILTREGFNLPADSVQDQTLERFSVEEVYDLLEKSPWDKKGFALGSPDLLTQDGMGNEQ
                     NSMPGALGQEQMRATDAHWRNAFEQASVMAETLLHGSLPASIQRELQTLKGGQLNWRH
                     YLWRYLVRTPIDFGEFDRRFVGKGLYLETLEGETVSVAVAVDTSGSINDEQIQLFLSE
                     VLSILLAYPQLNCQLYYADAVLHGPYELTPYASFPSPVGGGGTDFRPFFTKLAQDHSP
                     WVTTVAVYLTDGYGFFPSEPPSFPVLWVVTPGGLDSSYFPFGETVRLLGEA"
     gene            complement(18864..19865)
                     /locus_tag="VFQ23_22910"
     CDS             complement(18864..19865)
                     /locus_tag="VFQ23_22910"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9909516.1"
                     /translation="MTPAELANYLDGLIRHDIKLSTMIWGPPGIGKSSIVAQTAEAFE
                     MACIDLRLSQLAPTDLRGLPVAENGLSRWYPPEFLPREGKGILFLDELNLAPPAMQGM
                     AQQLILDRQVGSYRVPEGWFIWAAGNRKEDMASVYDMPSPLANRFLHLEVEPEFESFK
                     RYALDTGVHENIIAFLAFRTNLLHKLTPQQNAWPSPRSWVMASKLHRANLSVESAVGS
                     ATAQEFKAFVALYGSVPDLEAILRGEGKTIPFPSEPSLCYATIIGLTSRAKDAQSAYH
                     AFQWLVGAAKPEWVQLCAIDLFRMMRIKGMMEELNGLFLQDEQVQQFLTTYQDAMAL"
     gene            complement(19943..20311)
                     /locus_tag="VFQ23_22915"
     CDS             complement(19943..20311)
                     /locus_tag="VFQ23_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909517.1"
                     /translation="MGWKDWVVVILVALTAGWMLFDGTRALIAGDYVTPKSGKYAGQL
                     GPWSNLVKAIGIEPRSTSMKLIFVGYGLTALIVLACFMFGLSWARTVLIIVCILGLWY
                     LPIGTMTNLIALALLFIGGK"
     gene            complement(20319..20714)
                     /locus_tag="VFQ23_22920"
     CDS             complement(20319..20714)
                     /locus_tag="VFQ23_22920"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9909518.1"
                     /translation="MKPLIRKVDCLRLYVPDLEAGLSFYRDRLGHELIWRSPTAVGLR
                     MPDTDAELVIQTEEQRQEIDLLVDSADEAAAFIEGEGGKVIVPPFDIQIGRCAVVEDP
                     WGNPLVVLDISKGLFETDADGNIIGIVEP"
     gene            complement(20836..>21039)
                     /locus_tag="VFQ23_22925"
     CDS             complement(20836..>21039)
                     /locus_tag="VFQ23_22925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909519.1"
                     /translation="IWHTVEKVTPEEWEKYKRELKDAYQRMEKLFHENPTWNEDTMGG
                     ALSIVIHTAYHLGEIRQALCTLK"
BASE COUNT         5348 a         5068 c         5375 g         5248 t
ORIGIN      
        1 acatccccca ttgaggcgtg tacagctcac ttgtatattt gtggaaacct aataaggtgt
       61 taatttgaaa ggagtaagag aaatgaaatt caaaacaata ttgggagcta tgttcgtcct
      121 ggctcttatc atgacagcct gcgcgcctgc tgaaactgca tcccccaccg cgatgacgga
      181 gccgaccacc gaatcaacat caacggaacc ggcgatgacc gaatcacccg caatgactga
      241 gtcaccggct gtcacggata cacaagccgt tgctgaaacg ccgactgtga gtgtgcctgt
      301 gaccggtgaa gtgactgtcg aagttagcga gacaacagac tatggcccga tccttgtgga
      361 tggcaatggg atgtctctat acgtctttat tgctgatact caggatagtg gtaccagcac
      421 ctgtggtgat gatgatggtt gtgctaccga atggccccca ttgctgtgta gtgcagagat
      481 gagccttggt aacgataatg caaacaccaa cagcaataca aatgacaaca gtaactccaa
      541 caccaatagc aatgcgaact ccaacgacaa cacgaatacc agcggaacac ctggtgccga
      601 cgattgtgct tcggaaacga ttgctggaga aggtgtggac gccttgctgt taggcaccat
      661 tacacgtgat gacggctcca ctcaggtgac ctacaatggc tggccgctct attactttgc
      721 ggcagatact actcctggcg acatgaatgg tcagggcatc gatgagttcg gtggactctg
      781 gttccttgtt tcaccgaccg gggaagccat tgaacagtaa aaaataataa tcagtaaata
      841 gctggaataa gaacttcgtt cgcaatttga acgaagttct tatttttaat tccatgtttg
      901 gctattgaat tcacaatggt atctgactat aatagaggag ccccttaatg tagtacttca
      961 gtttcaatat tcgcattgaa ttggtatttc tgacaatgtc tgtaagcaat tcaacaagcg
     1021 caaaaaagac gtctcgactg accgctccta ttttagtcaa ttcaactgaa gtagagatta
     1081 tgcgcgcatt acgcagacag gggcgaattt ccagaactga aatttcaaac attacaggtt
     1141 ggtccaaggc caaggcatct caggagattc gccgcttggt gaataaaggc tatttgatag
     1201 agatcggtga aggggcatca cagggtgggc gtcggccgcg actgctcaga atcaataatc
     1261 agctgggttt tattgtcggg attgatatcg gagccacaag cctggacgtt gccctggcag
     1321 atgtctccgg actgattctt aagcgcagat ctgaacctac ggatgtcaag ttgagtcctg
     1381 tatccgtgct ggggcgctgc agcgaactgc ttcttgagct aattcaagac caaagtgtaa
     1441 accctcatca gatcctggga atcggtatcg gcgtacccgg acccgtggat tttgcccgag
     1501 gcgttttggt ggcacctccg ctcatgcccg aatgggaaaa ttttctcatt cgggattttt
     1561 ttaagaagac ctttacatcg gcgtttgtgg tggtggacaa cgatgtaaat atcatggcac
     1621 tgggggaaca acgatcgggt gatgcagaga atgttgatca cttccttgtg gtcaagatcg
     1681 gcaccggcat cggttgtggg ataatggcaa gcagaaaaat ccatcgtggc agcagtggca
     1741 gcgccggcga cattggccat atttgtgttg acacgaccgg accggtttgt cggtgtggta
     1801 atcggggatg tctggaggca atggccgccg gacccgcaat ctctgaaaaa gcgactcagg
     1861 ctgccagaga gcgaaaaagc aaattgttac taaaaatgat gcaaaataac ggaaatgtat
     1921 tgaccccgga agatgttaat atggcctgcc gtgaagggga tgaaactgcc ctggagatta
     1981 tccgttccag cggcaggatg attggcgagg tgctggctgg gctcgtgaat tttttcaatc
     2041 ccagtcaaat ttatattacc ggcggcatat ccaatttcgg caatcattta ctaattgcga
     2101 tcaagcgtgc ggtcttacgg cgttcactgc ctttggcaac ttcgggtctt gacattgttt
     2161 tttcacgaat gggttcggat gcgggcgtgc ttggagccat tatgctcgcg ttggaatatc
     2221 tcttcatcgt cgagaatgat ttacattttt ccttttagat tattggatca gaaaggaggt
     2281 gcaaggccgt tctaaaacaa agcacaggtc atctccatcc atactaagat cgaacgatga
     2341 cctctgaacg ccaggatgag aacagagagc tcagcctgga aggatcgtaa aactgatact
     2401 ctataaattc aaggagtgag acatgaaaca aagtaaatac cttacactgt tcagcttgct
     2461 aatcgtagcc agcgtcgtat tgaccgcctg cggaggcgcc gctgcaactg cgaccccggc
     2521 gccccagcaa gatggatttg tgattggtgt aagtaacaca ttggtaggta atggctggcg
     2581 cgagcaaatg atttgctcca tcaaagctga agcaaccgcc agcggactcg ttgataaagt
     2641 agttgtcgtt aatcgcaatg gcggcccaac tgagcagatt gccgatctcg aagggcttat
     2701 ttcccagggc gtggatgcta tcattatcaa ccctactgac cgcgaaggtt tgaatgcagt
     2761 cattgaaaca gcgattgcac aaggcattgt tgttgtggct gtcgatcagg ctgtaactgc
     2821 tgaaggtgcc tatgtcgtca ccaatgacca gactgcctac gctcagcttg gcgccgagtg
     2881 gctgtttgaa caaatgggtg gttccggcaa tgttgtggaa atgcgtggta ttgacggcgt
     2941 acctgccgac acagaccgcc ataatggttt cctggcggcg cttgaaaaat atccggacat
     3001 taacgtagtt gccgaagtgt ttaccggctg ggatccctcc gttggtgcac agcaggccct
     3061 cgacctgatt accaccaccc aagtggatgg tatctggaca tcaggtattg actataccgt
     3121 ggtagagcag ttcattgccg ccaatgctcc ctatgtaccg attgtaggtg ctgacaacaa
     3181 cggttttatt aaacagttga tcgaacttga aggtgatgga ctcactggtg ctgcagtcac
     3241 gaacccgcct gccattggtg cagttggcac ttcgattgcc ctgcaagccc tgaccggtaa
     3301 gaacccggag catgtagtta cattgacgcc taaggtgtat gctactggtg acagcgccct
     3361 taacgatttg ttcgcacctg acgaacagcc gggctggagc acatttgtta acatcgatcc
     3421 ctataccagt tattctggca gcgccgatgt ttctgcatgt aagggaccag gagaataaca
     3481 ctggttttac agagtgccgc acgcatgtct cacatgcgta tctaaacttg aaatttgaat
     3541 ttcagcttac acagcaattc aaaagtttac ttaataatga gacaagcggt agattgatca
     3601 ggtgccttga tgaccgagaa aattaatccg tatttactcg agaccaaagg cattgctaag
     3661 tcgtatggat ccgtcgtggc gttgacgtcg gttgatctcg tcgtccgcca cggcgaaatc
     3721 catgccttgt taggcgcgaa tggtgctgga aagagcacgc ttgtaaaaat acttgcgggc
     3781 gtacagtctg ccgatgccgg cgaattacag gtcaatggaa aatcggtgac ttttcattcc
     3841 ccggcggatg ctatggcagc cgggatttct acggtctttc aggacccttc actgatccct
     3901 gatctgacga ttgaacaaaa ccttcgtctt agcaatattg aagaatcgaa gttcagagaa
     3961 tggttggatt ggttcaacct gggtcagctt gatctttctg ctctggtgcg tgaacttccg
     4021 ctggagacgt tgcggattgc agatttagcc agagcggttg cccggaagcc acaattatta
     4081 ttgcttgatg aacttacagc ggctctgaca gctgaccagg ctgaacgagt atttaaattg
     4141 ctggctcatt ggaaggaaat tgggaattca gcagttctga tcactcatcg cctggcagag
     4201 gtgatgagta tttgtgatcg ggctaccatt ctgcggaatg ggcacaatgt agatgtgctt
     4261 gagacctcca aggtgaatga acaccaattg gtcgcagcca tgctcggtca attggcagaa
     4321 gcgggcagcg aggaaaccaa aggagatgtt gatcgcaacc agggtgaccc tgcgttcacc
     4381 gtaatggaat tgagctctgg ggatgtagta cgcgatgttt ccttctctgt aaaatcaggc
     4441 gagatacttg gcattgtagc tcttgaggga cagggacaag accgcctgtt cgagctgctt
     4501 tccggagata gacaaccatc ctcggggaaa attctggtac atggcatagc aaagcatttc
     4561 cgttcacctt ttgatgcggt ccaatctgga gttgttttgg tgccggggga tcggctgcgt
     4621 gctttgctgc ctgacctatc gatacgacaa aaccttgcca taccactctt caaccgaatt
     4681 cgtgagtggt ttggcctccc gcgtgatgaa aatgaaaaag tggcgggcgc aattaatcaa
     4741 ttatccattg atacaagggc tgcgtctcag gtacgacgac tgtctggggg aaatcagcaa
     4801 aaggtagtta ttggtcggtg gttagtttct ggttttgaaa ccttactgtg ttttgatcca
     4861 actcggggta ttgatatcca aaccaaacgt gaaatctata ttttgttacg aagattagca
     4921 tcggagggct ctgcagttct tctctatacc agcgaacttg cagaaatccc tttggtttgt
     4981 gaccgggtgt tggtgatgca tgccggaaaa attgtggctg aacaagatgc ggccactgca
     5041 accgaatcct ccatgctgac cgctgctcac ggcttggacg ttacaaaatg aacaaagtac
     5101 ataggactgg gaataataat ctgtggcaaa aaattctctc gcggaaccgc tgggttctcg
     5161 gcgcgtggtt attgcttggc gtattgttat tatggtattc cacactcatc ccggtatttg
     5221 gtgagtttca aatcacctcg atcacgaaaa acagtctgcc gttggtgtat ctggcaattg
     5281 ggcaggcaat tattgtaatt ggtgggggaa tcgacctttc gttaggttca ttgttattac
     5341 tgggcaatgt cattgcagcc cgctttatgg aagaccagtc acctggcgtt gcttttgtga
     5401 ttggattcgc catgatactc ggaatcgctg tccttaatgg catcaccggt tatgttatca
     5461 gtgtctccag gataccggat attgtagtga ctttggcgac cagttatata tggtctggca
     5521 tggctttatt gattttaagc tctccaggcg gaggcaccgc gccggaagtg cgctggctat
     5581 tttcggggtc ggaatccgga attgggggaa gcccaattat gccaatcctg atgttaatca
     5641 tccctaccct tttagttttc acgtatctta atcggagtca gaatggcttg tccatttatg
     5701 cgatcggtag cgacaaaacg gctgcccaat tagccggcct tgatgtgcgc aaggcaaagg
     5761 tactctccta tgcaattggc ggagcaatgg cgtcgcttgc cgggctggca accgtggctc
     5821 tgaccgggac cggtgatccg cgttttagta tcggagcgat tgccacactc aatagcgttg
     5881 cggctgttgt cctgggcggg gtggcgctca ccggtgggat aggatccgtg attggaatgg
     5941 tccctgctgc tgtgatattg atcatgttaa atccgatatt gagcgcaatg gggttcgacc
     6001 caaatagtgc acaagttgtt cagggcatct taattgcggc agtgatgatg gtcggcggtt
     6061 ttgttacttt gcgccgcgaa agagcagcat gacaaataaa acaaactcaa gcgagcctgg
     6121 aatgttaacg gccgattcaa agaccgaagt caaacgagct gggtttattt ctcgggtccg
     6181 aacgatagcc gaggaacgcc ctgctctatt gctcgtcggg gttttattta ttttggtagt
     6241 tgtaacaggc atcattcagc cgaattacat atctgtcgct gggattcgaa atacattatt
     6301 gctggcggcg ccccttggta ttttggctgg ggcacaaacc atcctgatgc tcaccggtgg
     6361 gattgacctg tctgtcacga tgattgctac tgggtcagct tatgtagcgg cgaaccaatc
     6421 tccaaatggc gcgttgatcg caattgtatt tggcatcctg gttggagtga ttgctggtac
     6481 ggcgaatggg attggtatca ccgtgtttcg agtcaatcca ttaatcatga ccttggccat
     6541 gtctgggatt ttactgggtc tatttacggg ttggacgctg accatcttac agggcgccac
     6601 gacggtagcc gactttatca aacttatggg aacaggctca ttttttggca atcgaattcc
     6661 ttatagtgtg gttatttgga tcgttgtctc catattatta atctggttat tggagcgcac
     6721 gggctgggga cggttgattt atgccatcgg taataacgaa ggcgccgtcc ggttggctgg
     6781 cattcgagtg tggcaggtgc gaattacggc ttatatggtt gccggcgtgc ttggctccat
     6841 tagcggcatc cttttagcgg gacgcactgg tgcggtcgat ttgcagcttg ccaacgcatt
     6901 tctgcttccc tcggttgccg cggcagtgat tggaggcacc tccatctttg gtggcatcgg
     6961 gaaatatacc ggaaccattc tgggagcgct catcctcagt gttcttaatt cgatgcttac
     7021 ctttcttaat gccgggcagg ccgttcagca catcgtctat ggcgtgattg tgctggcgtt
     7081 ggcctgggga tatgcaagtc ttacccgaag gacatgaaaa tttccattca ggagaaatat
     7141 gatgcataaa gtaacaatgc ttgggacagg cttgatcggt atgttttata ccaagacgct
     7201 gcaccggaat cgcagtctcg atcaggttgc cgttgtctat tcgcggaccg aagaacgtgc
     7261 aaaacagttc gcctctgaat ggaagatacc caaatatacg accgatctgg aagcggcgat
     7321 taatgatccc gatacggacg tggtggtgat tggcttaccc aataacttac atgaagaagc
     7381 agttcggctt gcaacccagg cgggaaaaca tgtcttatgc acaaagccgc tgggtcgaaa
     7441 cgcagaggaa gcccggcgta tgttgaaaat ggttgaagaa gccggggttt tcggcggcta
     7501 tctcgaagac ctggcgtaca cgccgaaaac cctcaaagcg atgcaatcag ttcggaacgg
     7561 cgcgatcggc aaggtattgt ggggtcgttc gcgcgaaaca cattccggcc cacacagtga
     7621 ctggttttgg aacaaggaaa tttcaggcgg cggcgcgatc gttgacctgg ggtgccactg
     7681 tattgagatt atccgaaatt tcatcggaaa agacgtacgc cccgtagaag tgatgtgctg
     7741 ggctgatacc caggtccacc caatcgatgc ggaagacagc gcaattggtc ttgttaaata
     7801 tgccagcgga gcgattggac agttcgaggt cagttgggct tttcgaggtg ggatggacct
     7861 gcgggatgaa atatcaggca ccaaaggtac aatctggctc aaccactgga tgcgaaccgg
     7921 ttttgagttg tttactgcgg agggtgaaaa agactatgtg tcagaaaaga cggaaagtga
     7981 aaccggctgg ctgtttcctg taggggatga gattggcggt ctgggatata ttacgatgtt
     8041 tgatgacatg cttgattcca ttgatggtgg taaacaacca atggaatcct tctatgacgg
     8101 ttatgtggtc aatgccatca tggatgcggc ctaccgttcc gccgcatcaa agcaatggga
     8161 accggtactc attgaagatt ggcgtggctc tgacgatgct gaaaccgaag tacaaatggc
     8221 agagtacgat gcggatcatt tcctgatcaa gcaggaaaaa atgccagatg gtaaaaccaa
     8281 gtacatctta aagcaaaagt ccagtggcgc gatcatacag aaggttatgt aattcaccat
     8341 aaatagattg aggattttta tgagtaattt gaatagtaaa acaattacct acgagcaact
     8401 tgcaaaagtg atcgatcatt cacttctgcg acccgaactg acagcagccg atgtcttggc
     8461 aggttgcgag cttgcagcac gctatcatgt ggcgaccgtt tgtgtgaagc cctgcgatgt
     8521 caaactagcc aaagaagcgc tcaaagattc tgatgtgcta gtcagcacag tggttggttt
     8581 cccacatggg agcaatctca ctgatgtcaa agttgcagaa gctgaaaaag ccatggacga
     8641 tggcgcactc gaactcgata tggtcctcaa tattggagga ctacgttccg ggaaacatga
     8701 cttcgtgcga gaggatatta aagctgtgtg tgacgcggct catgctcgcg gtgttaaggt
     8761 taaagtaatt tttgaaaacg cttatctcac ggatgaggaa aagatcacgg cctgcaaact
     8821 tagcgaagcc gctggcgcag actgggtgaa gacctctacc gggtttgcct cgggtggcgc
     8881 gacgcttgaa gacctgcgtt tgatgcgcgc caatgtcagc gaaaaggtgc agatcaaatc
     8941 tgcgggtgga gtccgcacgt tgcagtcctt actggatgtg atcgacgcgg gtgtgacgcg
     9001 ttccggcgcg acagccacgg caaccatcct ggatgatttc aagaagatga aagaaggaac
     9061 gccgcttact acgccggatc ctgattctgt ttcaggaact tattgaccct aaaagtcttt
     9121 aagaccttta gggtctaaac ctcaaaaaga gatactttat ggccaaacaa ctcacaaaac
     9181 aaaccattgg cgttggcgtg gcagggactg gctttatcgg tccggcacac gtcgaaggct
     9241 tgcgtcgaaa tgggattcgc gtcctcggac tggttggaag cacaggtgaa aaggctgagc
     9301 agaaggctgc tgagttgggt attccgcatg cgtatgcctc actcgatgaa atgctggcag
     9361 accctgagat tgacgttgtg cacctcgcta cgccaaatca tttacaccat ccacatgcca
     9421 gagccgcgtt attagctggc aagcatgtcg tctgtgagaa gccgctggca atgacgtctg
     9481 aagagtcggc ggacctcgtt caacttgcgg tagagaaaca actcgtcaat gcagtcaact
     9541 tcaacattcg aatgtacccg ctggtgcatc aagcgcgcag catggtgcaa agcggcgaac
     9601 tcggtgacct ctttatcttg cagggatctt atttacagga ttggctcctg ttcccaaccg
     9661 attggaactg gcggctggaa acgaatctcg gtggcacatt acgtgcggta ggcgatatcg
     9721 gttcacactg gcttgatctt ctcacgttta tcacaggctt acgcgtggaa gaagtctact
     9781 cagatttcaa gacattccat ccggttcgca agaaaccagc caaaccactc gaaacgttta
     9841 cgggtaaaat tctcacgccc gaggattaca tcgatcaacc catcagcacg gaagattatg
     9901 cgacaattct tctgcactat gaaaatggtg tgaccggcgt agtgactgtt tcacaagtct
     9961 cttcgggacg caagaatcgt ctcttttatg aaatcaacgg atcgaagtct tctcttggct
    10021 gggattcaga acggcccaac gagttgtgga tcggtcgtcg aacggaaccc aaccaaacct
    10081 tattaaaaga cccgtcgctt ttatccccgg aagcgcgcgc cgtgacatcc tatcccggtg
    10141 gtcacaacga aggcttccct gatacgttca agcagttgta cgccaaagtg tatgattaca
    10201 tcatctctgg cgattacacc aaaacaccag acttcccaac ctttgccgat ggtcactatg
    10261 aaatgcaatt atgtgaagcc attgaacgaa gcgcgcgtga aaagacgtgg gttaaagtca
    10321 aataaaggag tcaataaatg aaactcggat tcttaaccgc tgcactgcct ggtaacacac
    10381 ttgaacaagc tgccaaatgg ggagcagaga gtggctttga agcgattgag atcgcttgct
    10441 ggccacctgg aaaagcgaca cgccgatacg caggggtgtc tcatatcgat gttaatgagt
    10501 tggatgaggc gaaggcgaga gagattcgca cgatgctgga tgggtacggt ttgaccattt
    10561 cctcgctcgc atactatccg aatcctctcc atccggaact ggagcatcgc gaaatggtaa
    10621 ttaatcatct aaagaaggtg attgaagccg cagaattatt ggaagttcca attgtgggaa
    10681 ccttcattgg taaggataaa aataaaactg ttccacagaa tctggaagac tatgcaaagg
    10741 tctggccgcc cattgtgaaa tttgccaaag accacggcgt gaaaattgcg attgaaaact
    10801 gcccgatgct cttttcgtat gatgaatggc ccggcggcaa caatctggct gccagccccg
    10861 ccatctggcg ccagatgtgg gagatcattc ctgacgacaa ctttggcttg aatctcgatc
    10921 cttcgcatct catcctacag atgattgatt acgaacgtgt catcaaggaa ttttctgaca
    10981 agctattcca cgtccatgcc aaggatttgc atatcgatcg ggatggtctc tacaataacg
    11041 gtgtcttagc ccagggcatg ggctggcaaa tcccccgctt accgggtctc ggtgatgtag
    11101 attgggcgaa attcattgga acacttaagt ccgtaggcta tgattatgtt gtaagcatcg
    11161 aacacgagga ccgcaactac gaaggcgatg aggaacttgt aaagaaaggt ttctatttat
    11221 cgcgtgatgt actaaaaccg cttatccaat aatcaatcag tataaataat attgggcgag
    11281 gtgaaatcct cgtccgaact gtttaaaaat agtaagtgca attaggaatc atttaagtgg
    11341 ttccgcttgc agatattgcc aggttgtacg ttcctgtttt agttggcgct gccaataaac
    11401 atattggagc accatcagcg gtccaagcac aaccattacc ggaaatgttc cgaccaagag
    11461 accagagata aggcttagga tcaacagggt aatagcccca agaccgatca ccaaagcgat
    11521 catgccaaca gtacggccga acgcttttct catgaccaat ggaaataccg ccgccattac
    11581 cagatctcct aaaccgatac tgagccagtc gccttcagcg ttcacgagcc aggcgatctc
    11641 cggcgcgaac ggcagccctt ccaaacggat aaagagatca ttcatcaatg gtaacaaaga
    11701 ggtggcgatc agatcataga ccatcaacgc ggcgccaagg atggccgcat ggcgcgcttt
    11761 cattccgctc tgcgcccaaa ggtttgtaac accaattaca gatataacta gaacaatgtt
    11821 atttacagcg aagaaccaga tcgaagggat gccaggcaac aacgcggtag cgagatcaga
    11881 tccggcgagg agaatcgtca ccagccagat actccataca gaacgaaaca ctggctccca
    11941 ggtaaagtaa agaatgctga tgctggcaag cgctagcaag ctagcgagta tccagatagg
    12001 cacaagtaaa tacaagaagg ggaccagaat gatcgcacca atcatgaccg cgatatcgcc
    12061 caggttgaaa acgccgatgg gtggccgcgt catcacgtag cgccgaaaat atagccaact
    12121 gagaattgct gctgccgcga cgtaagccaa aacgacgaac gaagccaaaa ccatttggtc
    12181 acctcatctc aatcggcgaa cgggagtgaa taacgcttct ttaagatcac aacgcaatgg
    12241 taagggatgt ttgagttgac tcgggttttc cacgaggtgt agttccttta ttggcacact
    12301 atgctcttct aagttttgtt caatcttttt atgaatggaa gattctgctt ttcttaccaa
    12361 aacctccaca gcgacaccac ctggcactga ccaaaaacca cagcgcgcgg gcagaggcac
    12421 ttcttcgata gcttccagcg cctcacgaac ctgccgcgga tgggcccaac catcaggacc
    12481 gcggactgag aggcgcaact tgccttgcag atcggatgtg gccggccaat gttttaggtt
    12541 gcaggtcaat gggccgttga tcgcctgcac tacatcttct gtatcataac gaagtactac
    12601 acttgcctcg cggtatggag gaaatggcgt tgcaacgatc gttccgaatt ctcccagttt
    12661 aacgggctca tgagtatcag gatcaaggac ttccatcaaa ccctgcgaag gttcgaagtg
    12721 caagtgactc tctgcacagc gcgtcccact aaacggccac gtctcggtca tggcataacc
    12781 ttggataaat tcgacagaac cgaagagctt ctggcagcgc ttctttaacc cctcagtgac
    12841 aatctcgcca ccgacagaga tacgctccaa accgaaatcc gcagggctat atctgttgga
    12901 tagaccatac tcgaccagct cgcccaggta agaagcatat gtgttcagaa aactggcgcg
    12961 cgacttttta cctggcaaat gatgctcctc agaaagtaac atcaaggcat gggtcgggtc
    13021 caccaagcct gtctggtacc acaccgcgcc gatgcgttgg caggcttgcg agaagcaggt
    13081 gttgccgagc gtggcgcgcg aggaggtgct gatctgaaca atatcttcgg gcgtgatctg
    13141 accgctttgg agaaaggtga ttgctgttag cagccccgtc gcctggatct catgcgatgt
    13201 gaaatagaca ctggtcggct taccggttgt gcctgtcgtt gtagtgcgga acgcaggctg
    13261 agagccccgg cgcacaaatg cctcgggatc actgcgaagc gcctctttgg gtgtaattgg
    13321 aatccggggg gtgtcttcat actttagatt tgccgggtca atacccagct ggtcgaattg
    13381 ttgcttgtaa tagtccgttt cgtttacggc gcgcaatgcc tgagttcgaa agcgacgcag
    13441 ttggatctcg cgccgtgact cttcatccag ctcaggaccg cccaggaatt cggcgctgtc
    13501 agtaccaaga gtgccaaatt catgctgtgt tgccaggatt ccctcgacca gcctgtccaa
    13561 cgcgcgcagg tcaaaaggaa tggcgaagag cacagaagcg gcgtaacgca gttgggcaaa
    13621 ggcggtttca aacatgggtg atcgtgtgat tagagcaact caagaataaa aaagttatct
    13681 tcataaaaga ggagaggctg aaggcttctt ccatcagcca gtctttgagt attcattttt
    13741 ctaggcaagt gcccatttgc tcaggcacat cggtgtctta gttgagaaca agtccgaaac
    13801 cgacatttga gagcagggac agagcatagc ggatgtagcg gatggtgagc tttttcatgg
    13861 gtagagtctc cctagttgga ataattctga atgacttggg tttctttagt tcagtgacaa
    13921 tttgaaaccg acatttgaga gcagggacag agcatagcgg atgtagtgga aggttagctt
    13981 tttcatgggt tgggtctcct tttgtttttg tgaaaaccat tttgtttcaa cttatgacaa
    14041 tactatatca ggaagagacc cacgtttcat ccgtgaaaac cctataacca accctacaat
    14101 ttgtggaacc ctcaccactt cgacaagctc agtgcagcgc ctgccctctc ccagcggggg
    14161 agggggaagt cgtccctact gaataccgtt atcatcggcg tgagggaatg ttgtcctact
    14221 ctttccgtgc aagcgtatcc aaatcagatg gcgcttccgc caagcctttt gccaccgccc
    14281 agcccgcgat ctgcgcgcgg gaggtaaacc ccagcttgga caagatacgc gtaacgtgcg
    14341 cctccaccgt tttcaaacca acgaagagtt cccttgcaat cgtctgatta gatttcccct
    14401 gtgcgatttg cgcggctacc tcgcgttccc tctcagttag cccgccgtat ttctgcttct
    14461 ctacctcgcg ctgcgagggg aggatggatg ctccaatttc tggttttagt tcagacatag
    14521 gcaccgagag gacaaattga gattccgcca actcgatagc ttgctccagt gtcatggagt
    14581 aaccctcatt ccaggctttg gtgtaggctg aatcttccat ttgagagcgt atcgagtcaa
    14641 taatgggatt ttcatagtca cgcatccaaa aaggcggctt tactccacct ataaattcca
    14701 ttcctttatc tgaaaagcca attagttttg cggcttgctc ccctttccct cttaaggatg
    14761 caacgccagc aagaccgata agacaagcat gcgtaaacca cctatcgccg agttgcttat
    14821 agagccgtaa tccttctttt aatgctgcct ctgatttatc aagttcggca tagcgccatc
    14881 tgcaaatacc caaaacaaat aacgaataag caatagaagg cagctcattg agttcccgcg
    14941 ctgtttgcag acattcttcc aatgttgaaa acccatcaag aaaattacct actgaaacat
    15001 gatgccagcc caactcaatc atcatcccat tcgcaaattc cctaagacca agttcgcgat
    15061 atagccacaa ggcttcttca taatactgag tatctgaatt gtcgtcgcca cgttcgtaaa
    15121 tagtctctcc taagaattga atcgccagtg caacgtcgaa tttatcgctt atttcacgga
    15181 acaaagccac gctttcctcg ccgtgcctga tggcttgatc atattccccc atacgcgcca
    15241 ataaacggcc tgatatacgg attgctttgg caagtgtgtt ttttgataaa acatcgcgat
    15301 acttaagccc ggtctctaac cagtatcgtc cttctgaata aaaatttcga tcgatccaaa
    15361 acaggccaag cttggaggca agccgaacgc acttttcgat ctgctccgtc tccaacagcc
    15421 aggcaagggc agcccgaaaa ttgtctaggt catgctcaag tcggtccatc catttcgcaa
    15481 attcctttcc aaatagcagt ccacgttcgg catcctcagc gagtttaaga aaccaatcgc
    15541 catgtttttg ccgcaacgtc ttgtcctggc ctgtatcaaa taacttctcg cgcgcatact
    15601 ggcggatggt ttccaacatg tgatagcggg attcatgttc tacttcttct gtaataacca
    15661 gtgatttatt gacgagttgt tccaaccgat tcagaatatc ctcccttttg actgatccac
    15721 cttcacaaac ggactcggcc gcctcaagcg tccagccgcc tacaaaaacc gacaatctct
    15781 ggaacaaagc ttgttcgtct tctaaaagca ggttgaagct ccaatcaatt gcagcgcgta
    15841 aggtttggtg acgttcgagc acagtgcggc tcccccctgt gagtaatcga aagcggtcat
    15901 cgaggcgttt ggcaatttgc tctacagtca gcacacggat ctttgctgcg gccagttcaa
    15961 tggcaagagg gacgccgtcg agatggtaac aaacctgagc gagggcgggc gcattgtcat
    16021 tcgttacagt gaaggttgga acagcagacg tagcgcggtc gatgaaaagt tttaccgctt
    16081 catattggct cagcgagtcg actggaggca gatgcgatag atcgggtaaa tccaatgagg
    16141 ggacgcgata tgtaacctcg cctccaatcc ctaaagcctc gcggctgctt gcaagaatgc
    16201 gtgtatctgg cgcggcgcgt aagatacggt ctgccgtcct cgcgcaggca tccacaagat
    16261 gttcgcagtt gtcgaggacg atgagaatct tcttttcgtg aagatagtca cataacatat
    16321 caatcaccgg acgctgtggt tcgtcacgca agccgatggc aatcgccgtc gtacgcggga
    16381 caagtcgagg gtcaagaatc ggcgcgagtt ccacaaacca gacgccatcg ggatataaat
    16441 gcagcatttc tccggctgcc tcgagggaaa gccgagtctt gcctgtccct cccaggccaa
    16501 tcagagtgag taagtgcgtg ttttgtaaga gccgtttaac atcggctagc tctttttcgc
    16561 gccccacaaa acttgtcagc tgggtgggca ggttgtttgg aagattgtcg agtgtacgaa
    16621 gtggggggaa ttccgtcggt aaatcagatg cgatgacttg aaatactcgc actggcgagg
    16681 ccatgcccgc gaatttctgc atccccatat tgccaagacc aatctgttcc ggtaattgtt
    16741 cccgaagcaa ttgttcagtt acgctggaaa ccaggatttg tcctccatgt cccgcagaca
    16801 tcaatcgttg cacaaagctg agtgtggtgt aaccacgata gtcattgcca ttgaactctg
    16861 cttcgcctgt gtgaatccct atccgcacat aaatggtcgc ttcgccccag ggttcgtttt
    16921 gtaaatcctg ttgtgccctt acggccgctt tgagcgcatc gcctgcctta tgaaacgctg
    16981 cacaacatgc atcaccaatg acttggaaca cgaatccatt attaagttca attgcttcgc
    17041 gcaaaatcgc gtgatgacgc gcccgcgcgt catcccaaga atcgggatac tcgcgcgcca
    17101 gccttgtgct gttttctata tctgtgaaga gaaaggtaac agtgccacta gggttggagg
    17161 tcataattgc ctccctttga ttttacctat tatgacacaa atgatttgat ttgaaaatgt
    17221 aaacccgcga taatatgcgg ctgttgactc agaaaaagac ttcttggaat ttcagttata
    17281 ctccttgaac cttagtgaac ttgatatggc atctgaagaa cttcaacctc atttattcac
    17341 tggacgttgg atcggcgaga ctcaaggata tgtgatgcct gcacacatct gggagatcac
    17401 gcaacacgcg cttggcttcc tgatggtatc tacacgttgg gaaggacaga cagaggtggc
    17461 tggcttctcc gctaaagtag ttcccaacga actggccttt atcatttctg gaagcaaaac
    17521 ttttaaggcg accttagttg acaagcagca ctttgtaatc cctggttggt gtacaaacga
    17581 tgtgcgcggc gggatcggac ccgcttatga tgtggttttt tcacgtccag ggattgccga
    17641 acttactggc cgccaggcct acgcacagta tcttgaacat gctgttcaag aaaagaatta
    17701 ggcctcgcct aacaaccgca ctgtttcccc aaaaggaaaa tagctggaat ctaacccacc
    17761 cggtgtcacc acccacaaaa cagggaatga aggtggttcg gacgggaaaa acccgtatcc
    17821 gtcggtaaga tacactgcca cagttgtcac ccagggcgaa tgatcttgcg ccagttttgt
    17881 aaaaaacgga cggaagtccg tgccgccccc tcccacggga gatggaaagc tggcataagg
    17941 agtgagctcg taaggaccat gtagaactgc atccgcgtaa tataactggc aatttagttg
    18001 gggataggcc aggaggatgc tcaggacttc acttaagaaa agctggatct gttcatcgtt
    18061 gatggagcca cttgtgtcga cagccaccgc gacagagaca gtctcgcctt ccagcgtttc
    18121 caaatacaat cctttgccaa cgaaccttcg atcaaattca ccaaagtcga taggcgtgcg
    18181 taccagatag cgccagagat agtgccgcca gttaagctgc cctcccttga gggtttgaag
    18241 ctctcgctgg atggaggccg gcagggagcc atgtaacaag gtttctgcca tgacgctagc
    18301 ctgttcaaac gcgtttcgcc agtgtgcatc ggttgcacgc atctgttcct ggcctagtgc
    18361 cccgggcatg ctgttttgct cgtttcccat cccgtcctgg gttaataaat ccggcgatcc
    18421 caaagcgaag cctttcttgt cccagggaga tttttccaat agatcataaa cttcttcgac
    18481 actgaatcgt tccagtgttt gatcttggac gctgtcagct ggtaaattga atccctcccg
    18541 agttaatata ccgttgacca cgatatctgc tgcaatattc cagcgtttgg catcgcgtcc
    18601 agtcccgcgc gagacatgca agagggcggc atggaggact tcatgtaaga ggacagcttc
    18661 ctgctggtcg agagtgaggc gctcaaaaaa ttggggattg atcatgatct gccggccatt
    18721 ggttgcggca gtgggaatcg tatccacgga aatcgtctcc gcaaataata gcaacgaccc
    18781 tagaaatggc gtgcgtgaac ataatcgcgt tcgaagaaca ctgagaatag agtccagatc
    18841 aaatgtcgtg tccatgctct gccctacagc gccattgcat cctgatatgt agtcaaaaac
    18901 tgttggacct gctcatcttg aagaaagagt ccattgagtt cttccatcat ccctttaatg
    18961 cgcatcatgc ggaagagatc gatcgcgcac agttgcaccc attcgggttt ggcggcgccg
    19021 accagccatt gaaaggcatg gtaggccgac tgcgcgtctt tggcgcgcga ggtgagtccg
    19081 ataatggttg cgtaacagag ggatggttcg ctgggaaatg ggatggtttt tccctccccc
    19141 cgcagaatcg cttcaagatc cgggacgctc ccatacaaag caacaaaagc cttaaactcc
    19201 tgcgctgttg cgcttccaac cgcgctctca acactcaaat tggcccggtg tagtttgctg
    19261 gccatcaccc atgaacgagg cgaaggccag gcattttgct gaggggtcag tttatgtaat
    19321 aagtttgtgc gaaaagccag aaaagcaatg atattctcat ggactccggt atccaatgca
    19381 taacgcttga aactttcgaa ttcaggttca acctcgagat gcaggaaacg attcgccagc
    19441 ggagaaggca tatcataaac agatgccata tcttctttgc gattgccggc tgcccaaata
    19501 aaccagcctt cagggacacg ataacttccc acctgtcggt ccagaattaa ctgctgtgcc
    19561 atgccctgca tggctggcgg cgccagattg agttcatcga gaaacagtat tcctttacct
    19621 tcgcgtggta aaaactccgg gggataccag cgagagagac cattctcagc aaccggcagc
    19681 ccgcgcaaat ctgttggagc aagctgggat aggcgtaaat caatgcacgc catctcaaac
    19741 gcctcggctg tttgagccac aatactggat ttcccaatgc cgggaggtcc ccagatcatc
    19801 gtgctcaatt taatgtcatg gcgaatcagg ccatccagat agtttgcaag ttcagcaggt
    19861 gtcatatcat tctcttctac ttcgaaagtt tagcatggaa attgagccgg ttttgaaaac
    19921 gtaacgaggt tgataagcat cattatttcc ctccaataaa aagcagtgcc aaagcgatga
    19981 gatttgtcat cgtgccaatc ggcaaatacc ataaccccaa aatacaaacg attattaata
    20041 ccgtgcgcgc ccaggataag ccgaacatga aacaagccag gactattaac gcagtcagcc
    20101 catatccaac aaaaatcaac ttcatggatg tggagcgcgg ttcaattcca attgctttta
    20161 ccagattcga ccacggtccc aactgccccg cgtatttgcc actcttgggt gtgacgtaat
    20221 cgcctgcgat cagagcgcgt gtgccgtcaa agagcatcca ccctgcggtg agtgcgacca
    20281 gtatgacaac tacccaatct ttccagccca tagatttctc acggttctac aattccaatg
    20341 atattgccat ccgcgtctgt ttcaaataat cccttactga tatccaatac aactagtgga
    20401 ttaccccacg gatcttcgac gaccgcgcag cgtccgattt gaatgtcaaa cggcgggacg
    20461 attactttgc cgccttcgcc ttcgatgaac gcagctgcct catccgctga gtcgaccagc
    20521 aggtcgattt cctgtcgttg ctcttcggtt tggatgacga gttctgcatc tgtatcaggc
    20581 atacgcagac ctacagcagt gggagaacgc caaatcaatt catgtcccag gcggtcgcga
    20641 tagaaactca gacccgcttc gaggtcaggc acgtacaggc gcaggcaatc tactttacga
    20701 ataaggggtt tcataacatt atttcgcggt ttggtaagtg gttcatgctc agccacagag
    20761 ttcgctgaga cctttgagaa aatctgcttt tctctgtgat ctctgtggcg aagaaatctt
    20821 tgtaagaaat tcagtttatt taagagtaca cagggcttgc cggatctcgc caagatgata
    20881 ggcggtatgg atcacaatag acagcgctcc gcccatcgtg tcttcattcc aggttgggtt
    20941 ttcgtggaac agtttctcca tacgctgata ggcatctttt aattcacgtt tgtacttctc
    21001 ccactcttcc ggcgtgacct tttcgacagt gtgccagat
//
LOCUS       DASTJP010000781         6031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1097890, whole genome shotgun sequence.
ACCESSION   DASTJP010000781 DASTJP010000000
VERSION     DASTJP010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097890"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1315)
                     /locus_tag="VFQ23_22930"
     CDS             complement(131..1315)
                     /locus_tag="VFQ23_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9909520.1"
                     /translation="MNTQEIIEIETKYSAGTYVKQPVVIVRGQGASLFDADGVEYLDC
                     SSGHGAANLGHAHPRVAEAIYQQASTLITLFETYPNDKRAQVMESLASLVDGLDRVFL
                     CNSGTEAVEAALKFARLSTGRKNIVAAMRAFHGRTYGSLSATFNKKYREGFEPLVPGF
                     SHVAYNNIDALAEAVNEETAAVILEVIQGEGGVHPASAKYIQAALQICSERGALLIVD
                     EVQSGFGRTGRMFAIEHFDVIPDLLCCAKSLAGGVPMGAVLIGPAVNNLAPGVHSSTF
                     GGNPLACAAAIATLKVMEEENLSRQAEVKGAYLIDKLRRIESPNIREVRGMGLMIGIE
                     LKQKVSPYLKALQEKKIIALNAGMTVIRLLPPLVITYQQIDHLVDVLTEVLVSDLIAV
                     EA"
     gene            complement(1318..2148)
                     /locus_tag="VFQ23_22935"
     CDS             complement(1318..2148)
                     /locus_tag="VFQ23_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HET9909521.1"
                     /translation="MTQSITVVKLGGTDGVDFSAICRDAADLLHQGRQLVFVHGGSAE
                     ANSLGDALGAPPKMITSPSGYTSRYTDRKTLEIFLMAVNGKVNSLLTEQLQRLGVNAF
                     GMSGLDGRLLQATRKDSIQSIDAQSGKRKMIRDDYTGKIECVNQALLTVLLNAGYLPV
                     IAPVAVSEKGEALNVDADRAAAMIASAIEAETLILLTAVPGLMKNFPDESTLISQLTQ
                     SQLVEAGKSAQGRMKKKVLGAEEALRGGVNRVVIADGRIQNPITNALNGNGTVIQCTA
                     "
     gene            complement(2310..3350)
                     /gene="argC"
                     /locus_tag="VFQ23_22940"
     CDS             complement(2310..3350)
                     /gene="argC"
                     /locus_tag="VFQ23_22940"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET9909522.1"
                     /translation="MKTVSIVGGSGYGGGELLRLLLGHPCVDVQQATSRSHVGEYVYQ
                     THPNLRKRTQLKFSDPAQLTPVDILFLAQPHGQAQHHIEHFAELSPRIIDLSADFRLR
                     DAEVYEKWYGEKHSAPEWLSKFVYGLPEVHREDLKNANFVSGVGCNATAANLALLPLI
                     KADLLDFSSPIYVEIKVGSSEGGAEGNSGSLHAERANVIRTFSAFGHRHTAEVIQEMG
                     VTAERISLTMTAVDLVRGVLATAHAKVKAGVTTKDLWKAYRQAANENPFLRVVKEARG
                     VYRVPEPKVLAGTNYADLGFELDESNGHIVSLCAIDNLMKGASGSAVQSMNLMMGWEE
                     TLGLEFMGLHPI"
     gene            complement(3347..4219)
                     /gene="lysX"
                     /locus_tag="VFQ23_22945"
     CDS             complement(3347..4219)
                     /gene="lysX"
                     /locus_tag="VFQ23_22945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HET9909523.1"
                     /translation="MQKRLKIGVLYSRVRVEEKWIFQTLEKRGIDYDRLDDRTIHFDL
                     NHPAAWQEYDAVLERSLSYNSGLYATRVLNAFGVPTVNSAEVAEICGDKLITSAMLAR
                     DDIPQPHNATAFTPEAALDAIESMGYPVVLKPVIGSWGRLLAKVNDRDAAEAVLEHKA
                     TLGSVQHSVFYIQEYIEKPGRDIRAVVIGDRVLTAMYRKSEHWITNTARGGEGELCPV
                     TPEIEELCLRAAKAVGGGVLAVDLVEHPHRGLIVNEINHTMEFHTMQPLSGIDIAGEI
                     VNYVLEVAAKEYVS"
     gene            complement(4294..4464)
                     /gene="lysW"
                     /locus_tag="VFQ23_22950"
     CDS             complement(4294..4464)
                     /gene="lysW"
                     /locus_tag="VFQ23_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HET9909524.1"
                     /translation="MSTVTCPECAAAISLEEGTEVGEIIVCSDCGVDLEVVGLNPTAV
                     QLAPMDQEDWGE"
     gene            complement(4547..5920)
                     /gene="argH"
                     /locus_tag="VFQ23_22955"
     CDS             complement(4547..5920)
                     /gene="argH"
                     /locus_tag="VFQ23_22955"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET9909525.1"
                     /translation="MSTLWGGRFAQKLDSGAWDFNSSLAVDQRLALQDVQGSLAWADA
                     LHTADVLSDEEHGSISLGLATVKKEFLAGKFLFAPTDEDIHTAVERRLTELIGDAAGR
                     LHTGRSRNDQVATDFRLWMLEAIPSLDAALKDVQTALVEQAKLAGETLMPGYTHLQRA
                     QPILLAHWWLSHFWSLQRDHARLHDLTARVSVLPLGSGALAGVPFDIDRLSLAESLGF
                     GEVSQNSMDAVSDRDFAAEFLFCTAMIGIHLSKLAEQIVLYTSAEFGFFELSDAFSTG
                     SSLMPQKKNPDVFELTRGKAGTLIGLLTGLLVTLKGLPSTYDKDLQEDKAPVFQATDT
                     LLTVLPVIAGAIRTITTQAERLRSGIDSTMMATDLADYLVEKGVPFREAHGVAGRVVR
                     VAVENKVGLESMSLESYQAISPVFETDVYQVFDPMQSVERRNAIGGTSTQSVRNQITK
                     CRENLQA"
BASE COUNT         1432 a         1704 c         1401 g         1494 t
ORIGIN      
        1 catccacaaa agcctcatcg tagccaatcg cccgcattcg ttcgacaagc catgccaccg
       61 cattaagctc ttgtccagat gggctatagt gtgaaacgag tccaaccagc gtatcaaatt
      121 cgctcatgga ctatgcttcc actgcgatca aatctgacac caatacttca gttagcacat
      181 ccaccaaatg atcaatctgt tgataggtaa tcaccagagg cggcaataag cggatgactg
      241 tcatgccggc attcaacgcg atgatcttct tctcctgcaa cgctttgaga tacggcgaaa
      301 ctttttgttt caactcgatt ccgatcatca atcccattcc tcgtacttcg cgaatatttg
      361 gagattctat tctgcgcagc ttgtcaatca aataggcacc cttgacctct gcctgacgtg
      421 acagattttc ctcttccatc accttcaacg ttgcaatcgc tgcggcgcag gcaagcggat
      481 tcccgccgaa cgtggagctg tggactcctg gcgcgaggtt attcacagct ggcccgatca
      541 ataccgcgcc catgggcacg ccacccgcca gcgactttgc acaacacagc agatcaggta
      601 tcacatcaaa gtgctcgatg gcaaacattc tgcccgtccg cccgaacccc gattggacct
      661 catccacaat caataaagcg ccgcgctcac tgcagatttg aagtgctgcc tgaatgtact
      721 ttgcactggc aggatgtaca cctccctccc cttgtatcac ttccaggatc actgcagcgg
      781 tctcttcatt caccgcctct gccaacgcat caatattgtt ataggctaca tgactgaacc
      841 cgggcacgag aggttcgaat ccctcgcgat actttttatt gaatgtagca gaaagagatc
      901 cgtacgtcct tccgtgaaag gcgcgcatgg cagcaacgat attttttcgt ccggtagaaa
      961 ggcgcgcgaa cttcagtgca gcctcaacag cttctgtccc tgagttgcaa agaaagacgc
     1021 ggtccaaccc atcgactagc gatgcaagtg actccattac ctgtgcgcgc ttatcattgg
     1081 ggtacgtctc gaagagggtg atgagagtgg atgcctgctg atagattgcc tctgcaactc
     1141 ttgggtgcgc gtgaccaaga ttggccgcgc catgtccaga cgaacagtcc aaatattcca
     1201 caccgtctgc atcgaacaag gacgcgccct ggccgcgtac gatcacaact ggctgcttga
     1261 cgtaggtccc tgccgagtat tttgtttcga tttcgataat ttcctgagta ttcattatta
     1321 tgctgtgcat tggatcaccg ttccatttcc gtttaaggca tttgtaattg gattttgaat
     1381 tcttccatct gcaataacga cgcgattcac tccgcctcta agggcttctt ctgcccctaa
     1441 cacttttttc ttcatccggc cctgtgctga tttacctgct tccacaagct gactctgcgt
     1501 tagctgtgag atcagcgtgg actcatccgg aaagtttttc atcaatcccg ggacagcagt
     1561 tagtaggatc aatgtctccg cctctattgc agacgcgatc atggcggccg cgcgatccgc
     1621 atccacattg agcgcttctc ctttttcgga gacggccacc ggcgcaatga ccggcagata
     1681 tcccgcgtta agcaagactg tcaataatgc ctggtttaca cattcaatct tgcctgtgta
     1741 atcatctcgg atcatcttgc gtttgccact ctgtgcatca atgctttgaa ttgaatcctt
     1801 gcgcgtggcc tgtagtaatc ttccatccag accgctcatc ccaaacgcat tgactccgag
     1861 cctctgcaat tgctctgtca gcagcgaatt gaccttgcca tttactgcca tcaaaaatat
     1921 ttccagtgtc tttcgatcgg tgtatcggga cgtgtaccct gaaggtgatg tgatcatttt
     1981 cggcggcgca ccgagtgcat caccaagcga atttgcctct gcagaaccgc catgtacgaa
     2041 gaccagctgc ctgccttgat gcagtaagtc tgcggcatct ctacagatgg cagaaaaatc
     2101 tacgccgtcc gtgccaccga gctttacaac tgtgattgat tgagtcatta attctccgtc
     2161 tcaagtggtg gtcgagtgtt ccgagtgata acgaggaacg tatcgagacc acactgtaaa
     2221 cttgcattct tactctgaat tgatggtctc gactgctcac ctgcactgcg cgcaggcgca
     2281 agtgtgcgtc tcgaccatcg cgacattctt tatattgggt gcaatcccat aaactccaaa
     2341 cccagagtct cttcccagcc catcatcaga ttcattgatt ggactgcaga gccggaagcg
     2401 cccttcatca agttatcgat ggcacacagg gagacaatgt gcccattgct ctcatccagt
     2461 tcgaagccta gatcagcata gttggtgcca gccaaaacct tcggttctgg cacacgatag
     2521 acaccccttg cctccttgac tacgcgcaaa aacggatttt catttgcagc ctgacgatag
     2581 gctttccata gatcttttgt ggtcaccccg gccttcactt ttgcatgagc tgtcgccagc
     2641 acaccgcgca ccagatccac agcggtcatc gtgagtgaga tacgctccgc ggtcactccc
     2701 atctcctgaa tgacttccgc cgtgtggcga tgaccgaacg cagaaaaggt acgtattaca
     2761 ttggcgcgct ccgcatgcag cgaacctgaa tttccttctg cccctccctc cgaggaaccc
     2821 actttgatct caacataaat gggagaagag aaatcgagca agtcggcttt gatcagcggc
     2881 aggagcgcca gattagcagc agttgcatta caccctacgc cactaacaaa atttgcattc
     2941 ttcaaatctt cgcgatgcac ttcaggcaat ccatacacaa atttacttaa ccactctggc
     3001 gcagagtgtt tctcgccata ccacttctcg tagacttctg catcccgcaa acggaagtct
     3061 gcagagaggt caatgattct gggtgatagt tccgcaaagt gttcaatgtg atgttgcgcc
     3121 tgtccgtggg gttgcgcaag aaataagata tcgacagggg taagttgagc gggatcactg
     3181 aatttaagct gcgtgcgttt tcgcaaattt ggatgagtct gatagacata ttcccccaca
     3241 tgagaacgag aggtcgcttg ctgaacgtca acacagggat gacccaacaa caaacgcaac
     3301 agctcgcctc ctccataacc tgacccgcca acaatggaaa ccgtcttcat gaaacgtatt
     3361 ccttcgcagc gacttccaaa acatagttga cgatctcacc agcgatatcg atgccgctca
     3421 acggctgcat ggtatggaac tccatggtgt gattaatctc gttgacgatc aggccacgat
     3481 gtggatgttc gaccaggtcc accgccaata cgcctccacc taccgctttg gccgcgcgca
     3541 ggcacagttc ttcgatctca ggcgtgaccg ggcacaactc cccttctccg ccgcgcgcgg
     3601 tattcgtgat ccaatgttca gacttgcgat acatggcggt caatacgcga tcgccgatca
     3661 ccaccgcgcg gatatcccgc ccgggctttt cgatatattc ctgaatgtaa aacaccgaat
     3721 gctgcacgga gcccagcgtg gctttatgct ccagcacggc ctcggccgca tcgcgatcat
     3781 tgacctttgc cagcaaccgt ccccatgaac cgatgacggg tttcaacact actggatagc
     3841 ccatggactc aatggcatct aatgctgcct cgggagtgaa ggctgtggcg ttgtgaggct
     3901 ggggaatatc atctcgtgca agcatcgcgc tggtgatcaa tttgtcacca cagatctccg
     3961 ccacttctgc ggagttcacg gtgggaacgc caaatgcatt caacacgcgc gtcgcgtaca
     4021 gaccgctgtt gtaactgagg ctgcgttcca gcacggcatc atactcctgc cacgccgcag
     4081 gatggttcag gtcaaaatga atcgttcgat catcgagccg gtcatagtca atgccgcgtt
     4141 tctcgagcgt ctggaagatc cacttttctt ccacgcggac acgagaatat aagacgccaa
     4201 ttttcaatcg cttttgcata agttctcctt gtcagaccat ggaccatgga caatcgacca
     4261 ttgattccgt ttccatagtc tattgtccat cgtctactct ccccaatctt cctgatccat
     4321 cggtgcaagc tgcacagcgg tgggattcaa tcccactact tccagatcga caccgcaatc
     4381 tgaacacacg ataatctcac ccacttcggt tccttcctcc agcgaaattg ctgcagcaca
     4441 ttccggacac gttacagtag acatgcttca ttctcctttg atgattctgc cgcttacatc
     4501 actactcacc agacttttga aatggattgc atattggatt tccatttcag gcctggagat
     4561 tttcgcggca cttcgtaatt tgatttctaa cggattgggt actggtgccc ccaattgcat
     4621 ttcttctttc aacggattgc atcggatcaa agacctgata cacatctgtt tcaaacacgg
     4681 gactgatcgc ctgatacgat tcaagtgaca tactctcaag accgacctta ttctccactg
     4741 ccacgcgtac aactcttcct gcaacgccat gcgcttcacg aaatggaacg cctttttcga
     4801 ccagataatc tgccaaatca gtagccatca tggttgaatc gattccgcta cgcagtcgtt
     4861 cagcctgagt ggtgattgtt cgtatcgcac ctgcgatgac aggcaataca gtgagtagcg
     4921 tatctgtcgc ttgaaaaacg ggcgctttgt cctcctgcag gtccttatca taggtggatg
     4981 gcaatccctt caaggtgaca agcagtcctg tgagcagacc gattaatgtg ccagccttgc
     5041 cgcgtgtcaa ttcaaagaca tctggattct ttttctgcgg catgaggctt gagcctgtgg
     5101 aaaaagcatc cgatagttca aagaacccaa attcagcact ggtatacaac acgatttgtt
     5161 ctgccaattt gctcaggtga attccgatca tggcggtaca gaacaaaaac tcggccgcga
     5221 aatctcggtc ggagacggca tccatgctat tttgtgatac ttcgccaaag cccagggatt
     5281 ccgctaaaga gaggcgatca atatcaaacg gaactcctgc aagtgcgcca ctccccagcg
     5341 gcagcaccga tacccgcgcg gttaaatcat ggaggcgcgc gtggtcacgt tgtagtgacc
     5401 aaaagtgact gagccaccag tgagccagca atattggttg agcacgctgc agatgagtgt
     5461 agcccggcat gagggtctca ccggcgagtt ttgcttgttc gaccagtgca gtttgtacgt
     5521 ctttcaatgc agcatccaaa gagggaatcg cttccaacat ccacagcctg aaatcagtcg
     5581 cgacctgatc attgcggctt cgacccgtgt ggagccggcc tgctgcgtct ccaattaatt
     5641 cggtgaggcg gcgttcgacc gcggtgtgga tatcttcatc ggtaggcgcg aagagaaact
     5701 tccctgcgag gaattctttt ttgacggtag caagcccgag cgagattgag ccgtgctcct
     5761 catctgaaag aacgtctgcc gtatgtaaag catctgccca ggcaaggctt ccctgaacat
     5821 cctgcagtgc caggcgttgg tcaacggcca gagacgagtt gaaatcccag gcgccagagt
     5881 caagtttttg ggcgaagcgt ccgccccaga gtgtggacat cctaatctct cttgaagcgc
     5941 gaataatctg gcttgggcat atcgagaccg gacacgggca gtccattcag cgcacggact
     6001 ttaagactca aaccaaagag ccggataaac c
//
LOCUS       DASTJP010000782         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_619814, whole genome shotgun sequence.
ACCESSION   DASTJP010000782 DASTJP010000000
VERSION     DASTJP010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619814"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFQ23_22960"
     CDS             complement(<1..1100)
                     /locus_tag="VFQ23_22960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909526.1"
                     /translation="MQNLVRSERSLFWFSIAIALLIGVITVRNYGESWDELQLYKYAD
                     NSLKAYATWPRTGEIPITGGYFENYGPAFMMFSKLTTQALKSIGFRWMEVDIHHLLYF
                     LTFLIGLWSFFEICRRWVGQNAAIGATLLFASQPLLWGHAFINPKDTTLLSLMLLSVH
                     LGLKMYDSLFSSQADSALDRLTSAWGNLVRSKKRLLVISTSIWIVAILVLFGGTPLWH
                     AWFDSSVRATASGETVLLSPIILGAAANFQTVEPQIYVQKFFVAFLQARAGFFWVSTI
                     VLFWLYFRYFPSVFSALGIILPASIALGFTSSIRIFGPLAGILVAQHAIRNSGKRSIL
                     VIAIYAVLAIFVMYATWPYLWSDPIGKLVETIT"
     gene            complement(1119..1973)
                     /locus_tag="VFQ23_22965"
     CDS             complement(1119..1973)
                     /locus_tag="VFQ23_22965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909527.1"
                     /translation="MLDSINKIVSGKRQAIVVGIGAAVALSFLLAPVVPPAIDWTLTF
                     RPATLTLLSGLSPYGRVYYVHAPWTLIPLIPLAVLPEQVGRVLLFFCGLASYAYVAHK
                     LGAKPIAVAFLLISPLVMHVLLNGNMDWLAILGFVLPPQIGLFFISIKPQIGIAVAPF
                     WLIQAWREGGWRQVLKTFAPFTIVLLLSFVPFGLWPLETIREDIVGVSFNASLWPLSI
                     PVGLALFITSIRKQRIEYAMAASPCLSPYLLFYSWGGALLAIISSVPETIAAVIGLWI
                     LVIIRFFS"
     gene            complement(1984..>2342)
                     /locus_tag="VFQ23_22970"
     CDS             complement(1984..>2342)
                     /locus_tag="VFQ23_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HET9909528.1"
                     /translation="RFDKLQAKHDYTEAYFTHGLAVQAAEATADYLHEHIRREMGIAE
                     NQGKRYSWGYPAIPELEDHTKVFELLPAVTTDLGIRLSTAYQLIPEQSTAAIIVHHKN
                     AKYYSVGESRVEQLMR"
BASE COUNT          685 a          562 c          597 g          498 t
ORIGIN      
        1 gttatcgtct caaccaattt gccgattgga tccgaccaaa gataaggcca ggtagcgtac
       61 ataacaaaga tcgccagcac agcatagatg gcaataacca ggatggatct cttcccggag
      121 ttgcggattg catgctgcgc cacaagaatc cctgcaagcg gaccgaagat gcgtatcgaa
      181 ctcgtgaatc ccagcgcgat ggaggcgggc aaaattatcc ccagggctga gaagaccgag
      241 gggaagtatc taaaatagag ccagaataaa acgatcgtgg atacccaaaa gaatcctgcg
      301 cgtgcctgaa gaaatgccac aaagaatttt tggacataaa tttgaggttc aactgtttgg
      361 aaattcgctg ccgccccaag gatgatggga ctcagcaaca ctgtctcacc gctagctgtc
      421 gcgcgcacag acgagtcgaa ccaggcatgc cagagcggag ttccaccaaa tagcacgagg
      481 attgccacaa tccagatcga tgtggaaatc acaagcagcc gcttctttga tcgaacaagg
      541 tttccccagg ctgaagtcaa cctatccaac gcagaatcag cttgagaact aaagagtgag
      601 tcgtacatct ttaatccaag atgtacactc aataacatca aagaaagtag agttgtatcc
      661 tttggattaa taaaagcatg accccacaag agtggttgtg aagcaaatag cagagtcgcg
      721 ccgatagcgg cgttctgacc cacccatcgc ctgcaaattt caaagaacga ccacaaaccg
      781 atgagaaatg tgaggaagta aagcaggtga tgaatatcga cttccatcca acggaaacca
      841 atgctcttga gcgcctgcgt ggtgagtttg gaaaacatca tgaacgcagg accataattt
      901 tcgaagtacc cacctgtgat cggaatttca ccggtgcgcg gccaggtagc atacgccttc
      961 aaggagttat cggcatattt gtagagctgc aattcatccc acgattctcc ataattacga
     1021 acggtgatga ctccaatgag caatgcgatt gctatggaga accagaatag ggagcgttca
     1081 gagcggacaa ggttttgcat taaatcatca caggaggctt aactgaagaa tctaattatt
     1141 accaggatcc aaagcccgat gactgcggca atggtttcgg gaaccgatga aataattgca
     1201 agcagcgcgc cgccccagga gtaaaacaac aagtaaggag ataggcaggg cgaggctgcc
     1261 atcgcatatt caatcctttg tttacgaatg gaggttataa acagcgccag cccaaccggg
     1321 atggataggg gccagaggct tgcgttgaaa gagactccta caatatcctc acgtatggtt
     1381 tcgagaggcc ataatccaaa aggtacaaac gaaagtaaca acacgatagt gaaaggcgca
     1441 aacgttttta gcacttgtct ccacccaccc tcacgccagg cctggatcaa ccaaaatgga
     1501 gcaactgcaa ttccgatttg cggtttgatc gaaataaaga ataaaccgat ttgaggcggc
     1561 aggacaaaac ccaaaatggc aagccaatcc atgttgccgt tcaagagcac gtgcattacc
     1621 agtggtgaaa tgagcaggaa agcgactgca atcggtttgg cacccagttt gtgtgcgaca
     1681 taggcatacg atgccagccc gcagaagaaa agcagcacac gcccaacctg ttcgggaagc
     1741 acagcaagcg gaattaacgg aattagtgtc cagggcgcgt ggacgtaata caccctgcca
     1801 tacggagaaa gccctgagag cagggtgagc gtcgctggtc ggaaggtgag cgtccagtca
     1861 atggcaggcg gcacaacggg cgcgagtaaa aaactaagcg ccaccgctgc gccgatccca
     1921 acgacgatgg cttgcctttt tccggatacg atcttattga ttgaatcaag catggaactt
     1981 catctatctc atcaattgct caacgcgtga ttctccaacg ctgtagtatt tcgcgttctt
     2041 atgatggaca atgatcgcgg cagtggattg ctcaggaatc aactgatatg cagtactgag
     2101 tctaatgcct aaatctgttg taaccgcggg caataactca aagacctttg tatgatcttc
     2161 caattcgggt atggcggggt aaccccatga atagcgtttg ccttggtttt ctgcgatgcc
     2221 catttcacgg cggatgtgtt cgtgcagata gtccgcggtt gcctcggccg cctggacggc
     2281 aagtccgtgc gtgaaatagg cttcggtgta gtcgtgcttt gcctgcagtt tatcgaagcg
     2341 ct
//
LOCUS       DASTJP010000783         7605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1110188, whole genome shotgun sequence.
ACCESSION   DASTJP010000783 DASTJP010000000
VERSION     DASTJP010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110188"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..1198)
                     /gene="surE"
                     /locus_tag="VFQ23_22975"
     CDS             complement(353..1198)
                     /gene="surE"
                     /locus_tag="VFQ23_22975"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET9909529.1"
                     /translation="MNKHILVTNDDGVLAPGLLALTQEMRKLGKVSVLAPDRNWSGGG
                     HVKTLDRALRVKEVRLADGTQAFASDGAPSDCVALATLGYFKEPIDLVVSGINAGANL
                     GHDVTYSGTVTAAMEAVIAGLPGIAVSLETIEEHIGDMDYTPAARIAGKIIQQVIENG
                     LSHETLLNVNVPLLAEEKIRGIKVTRQGLRVYHSRLDERTDPRGRPYYWIGGDAPTGV
                     PERGTDVGALAEGFVSVSPLQLDLTAYRTLTDLNTWRWDDESNPYLTSQMIFSDQTLK
                     LKTEG"
     gene            complement(1316..2023)
                     /locus_tag="VFQ23_22980"
     CDS             complement(1316..2023)
                     /locus_tag="VFQ23_22980"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9909530.1"
                     /translation="MGLADLHIHTIYSYDGTASVPAVLTQARKQGLDVIAITDPDEIA
                     GSLKALALAPKYGVDVIPGIEITTSEGDLLALNITEKVERNLPLIETILRVGELGGFC
                     IAPHPMADGIGMKSLSAYSILKALRNNDVADILIGIETYNATTIDKMSNHYAHILSEH
                     LDLAQIGNSDAHVVEAIGLGATEFSGKTVPALLKAIRKQKTTVHKKNEWTPVRILGSW
                     AARYVGSTFARRSALAQ"
     gene            complement(2388..3608)
                     /locus_tag="VFQ23_22985"
     CDS             complement(2388..3608)
                     /locus_tag="VFQ23_22985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909531.1"
                     /translation="MNSFFWVITILLVMIAAWFAWRYYNLRRSIDEFAGQAREQNITT
                     ETKELQNLSSAIASLVSTFDAQHSAVDAERARLAAVLDQMTDGVLIADSQGLIHFANP
                     AAGRLFQTSNPTQRSIAEVIRNHQLVEAWRRCQQTGQMQTESVEVPTRHQFLQLVVIP
                     DTHASGSLLLVQDLTRLRRLETVRRDFVSNLSHELRTPLASLKALAETLHEGALDDPP
                     AARRFIEQIQVEVDALTQMVNELLELSKIESGRFMLDLAPVAACDLLDSASRRMLLQA
                     ERAGLRLRVDCASDLPKVYIDSQRLEQVLVNLIHNAVKFTRAGGEVVLSAQPADGSVR
                     FGVCDTGIGIPEDEVSRIFERFYRVDKSRTGNGTGLGLSIAKHIIEAHKGKIWAESRE
                     GQGSMFYFSIPITP"
     gene            complement(3612..4322)
                     /locus_tag="VFQ23_22990"
     CDS             complement(3612..4322)
                     /locus_tag="VFQ23_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909532.1"
                     /translation="MPDNILVVEDEPALRDTLIYNLKKDGFNVEAVGDGRSALESARR
                     LQPDLILLDIMLPELDGFEVARILRKEMSTPILMLTARDDEIDRVVGLEVGADDYLTK
                     PFSMRELMARVKAQLRRTRLLREELEKSPQPEKKQETLSFNDLVINITRREVTLHNIP
                     LQLKPKEYELLEFFAAHKGQMLSREFILERVWGWDFIGDSRTVDVHVRWLRQKVEEDP
                     GSPTRIVTVRGGGYRFEG"
     gene            4722..6527
                     /locus_tag="VFQ23_22995"
     CDS             4722..6527
                     /locus_tag="VFQ23_22995"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HET9909533.1"
                     /translation="MTREFYFSWQWDLRSSPEAIWPYVADTNRFNRDTGQPEVEMLDN
                     KMGTKNARMKLPFIRVEWEEEPFKWTYPYSFGVLRIYRKGPIDEMRVQADFEPLATGG
                     TRIKYQTWIKTSNILAQLGIPFGIGVIAKNRFERAFRFYDRIAEKDKSQKLISIGRPH
                     GLNARERARFKSLSETVLRQGTSAALVARLESFLNEADELSLQRIRPYALADHWGENR
                     RAVLEMFLRATRAGIFDMSWDILCPSCRGTTEGRTNLGEVRSQSHCNTCQIDFTTNFD
                     HNIEVIFQPNPSVRPVEFTAAFCVGSPQLQPHVVMSQSISSLRSLPVNVKLEHGRYTL
                     RASNIAGSISLFADAAGSPSSDIRVTSFGWPAEQQHVSLLPTLNLINATDSDQTFQLE
                     RTAWSDQASTAADVTALQVFRDLFATEVVRPGEEISIGSVTLMFTDLRDSTRLYRKIG
                     DASAFGRVREHFEILEKSIAEQGGAIVKTMGDAVMATFRHPIDGLRAVWNAQKQIAER
                     GEPMLWLKVGLHKGPCIVVNLNDRLDYFGSTVNITARLPGFSQGGELILSEAFYGDPE
                     IQDFLTQDIKPSALTKFTGNVKGFDEPFAMWKLAV"
     gene            complement(6579..7556)
                     /locus_tag="VFQ23_23000"
     CDS             complement(6579..7556)
                     /locus_tag="VFQ23_23000"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9909534.1"
                     /translation="MSTTLEAVRPIELDEIKEARERIANTIMRTPLVRLELGPGYPDI
                     RLKLENLQPINAYKLRGAANAVGMLSEAERRRGVWTISAGNAGQGVAYAAKKAGVPCT
                     VVAIETAPLSKLERMKALGANLVLVSYDDAWKALDARSFPGVEGTFVHPFDDHNFIAG
                     HATMGLEILEDAPDVVTVIAGIGGGGLITGVGSAIKALRPDVQLWGAEPETAAPAALS
                     FSMGSPQVFKEWQASFVDGAGGKSLFPRMWERIKPLADGFIVVSLEETKRAMRIMAEK
                     ARIIAEGAGALPLAAALTGKAGQGPIVAIVSGGNIDLKKFSELIESVAG"
BASE COUNT         1820 a         1977 c         1878 g         1930 t
ORIGIN      
        1 agaacaggtt aacaacttgt taaagttaag ctcctaaggg actcaaagtc ccttaggagc
       61 tataatccat atgtaaaata attgcccgcc atctcaccag aggcgggcaa tttccctact
      121 agggaacgga aggaggagaa aacagggaac gttgtgtttg accagtttgc gaaaacggat
      181 caaattacag cgtaaatccc tcataaacta attattgtag agttatgctc tttcctgcgc
      241 ttcttcatcg ttgcggtggg catcaataaa agttccaatg aaaattagca gtgtaaacag
      301 tgcaatgaca acgtaaattc caaaacaggt tcctaacata gtagcctcca gttcatcctt
      361 cggttttcaa ttttaaagtt tggtcagaaa atatcatttg tgatgtgaga tatggatttg
      421 actcatcgtc ccatcgccat gtattcaggt cggtcaaggt gcgatacgcg gtgagatcta
      481 attgcagcgg tgagacggaa acgaatcctt cagcaagcgc gccaacgtct gtgccgcgtt
      541 ccgggacgcc tgtcggcgca tcgccaccga tccagtaata gggtcggcct cgaggatccg
      601 ttcgctcatc cagccggctg tgatagacac gtaagccctg ccgtgtgact ttgatgcctc
      661 tgatcttttc ctccgctaga agtggcacat taacattcaa aagggtctca tgggaaagac
      721 cgttctcgat gacctgctgg attatttttc cggcaattcg cgctgcgggt gtgtaatcca
      781 tgtcaccaat atgctcttcg atcgtttcaa gcgagacggc gatccctggc aagcctgcga
      841 tgaccgcttc catcgccgca gtgacggttc cggagtaggt aacatcatgc cccaggttcg
      901 cgcctgcatt gatgccagag acgaccaggt caattggctc tttgaagtaa ccaagtgtag
      961 ctagcgccac acaatctgag ggagcgccat cgctggcgaa ggcttgcgtt ccatcggcca
     1021 ggcgaacttc cttcactctc aacgctcgat ccagcgtctt aacgtgtccg ccgcctgacc
     1081 agttgcgatc cggcgcaagg acgctgacct ttcccagctt tcgcatttcc tgcgttaacg
     1141 ctagcagccc gggagcaagc acgccatcgt cattggtgac gaggatatgt ttattcattt
     1201 tctatttacc ttctataaga aacgtatgtc gtttctgtaa tgcgtcgatt gtgataacca
     1261 caatcaaact aaaaatcgtt agagcaagaa gtacaaattc cattcgttct ccgttttact
     1321 gtgccagtgc agagcgcctg gcaaacgtgc ttcccacata gcgggcggcc cagcttccaa
     1381 gaatgcggac cggtgtccat tcattctttt tatggacggt tgtcttctgc ttccgaatcg
     1441 cttttaggag agctggcact gtcttaccgc tgaactctgt ggcgccaagc ccgatcgctt
     1501 ccacaacgtg agcatcactg ttcccaatct gtgccagatc caaatgttca gacaggatgt
     1561 gggcgtaatg gttgctcatt ttatcgattg tggtggcatt gtatgtttcg ataccgatca
     1621 aaatatcggc tacatcgttg tttcgcagcg cctttaagat cgaatacgca ctcaggctct
     1681 tcattccgat accgtctgcc atggggtgag gggcgatgca gaaaccaccg agctcaccaa
     1741 ctcgcaatat tgtttcgatc aatggcaaat tgcgctccac cttctccgtt atgttcagcg
     1801 ccaggagatc gccctctgac gtcgtgatct caatgccagg gattacatct acgccgtatt
     1861 tgggtgccag tgccagcgct ttcagtgaac cggcaatctc gtcagggtca gtaatggcaa
     1921 tcacgtccag tccttgtttt cgagcttgag tcaacacagc gggcacagag gccgtgccgt
     1981 cgtagctgta aatggtgtgt atgtgtaaat cagcaagtcc catttgttct cctttttata
     2041 caatcccagt gtagcagggg ggagttaagg ataaatcaag gacgttttaa gagttggtta
     2101 acagaattcc ttcaaaaccc aaaggattaa cgcacaagag cctgggagga gaccaggctc
     2161 ttgtgaggag gagaagagtg aaagtcttct tggtgcattc aggattgctt ttttgccccg
     2221 gcctgatgat attgaaaagc aagtcgcttc tgcttcgtat caatgataaa gacgatcagc
     2281 attgcagtga tgttcacgac caagaaaatt tccatgccca taggatagca tggagtccct
     2341 attctgtgct ttacagcgag tttgccaata caaaacaaaa atgcaattta tggagtgatc
     2401 gggatggaaa aataaaacat gctcccttga ccttccctgc tttctgccca tatttttcct
     2461 ttgtgcgctt ctataatatg cttcgcaata gataagccca gtccggtgcc atttcctgtg
     2521 cgggatttat cgactctata gaatctctca aagattcttg aaacttcgtc ttctgggatt
     2581 ccaatgccgg tgtcgcacac tccgaatcgt acagagccgt ctgctggttg tgctgaaaga
     2641 acaacttcgc ctccggcgcg tgtgaacttg accgcgttat gaattagatt taccagcacc
     2701 tgctccagcc tctgcgaatc aatgtagact tttgggagat cacttgcaca gtccacccgc
     2761 agacgcagcc cggcgcgttc ggcttgtaat agcatacgcc tggacgctga gtcgagcaga
     2821 tcgcaagccg cgaccggtgc aaggtccagc atgaaacgcc ctgattcgat cttggaaagc
     2881 tccagcagtt catttaccat ctgagtcaat gcatccactt ccacttgaat ctgctcaatg
     2941 aatcgacgcg ccgcgggagg gtcatccagc gcgccttcat gaagagtttc agccagtgct
     3001 ttaagagatg ctagtggagt gcgaagttca tgggagagat tgctgacaaa atctctgcgc
     3061 accgtttcca ggcgtcgcag gcgggtgaga tcctggacca gcaaaaggct gccgctggca
     3121 tgggtatcag gaatgacaac cagttgcaga aattgatgac gggtgggcac ttcaacggac
     3181 tcagtttgca tctggccggt ctgctgacaa cgacgccacg cttcgacaag ctgatggttg
     3241 cgaatcactt ccgcgatact gcgctgtgtt ggattggagg tttgaaagag ccggcctgca
     3301 gcaggatttg caaaatgaat tagcccctga gaatctgcga taagcacacc atcggtcatt
     3361 tggtcaagca cagccgccag acgagcgcgc tctgcgtcaa ctgcggaatg ctgtgcatcg
     3421 aaggtcgaaa ccagggaagc aatggcactg gaaagatttt gaagttcttt agtctcggtc
     3481 gtgatatttt gttcgcgtgc ctgacctgca aattcatcga tactgcgtcg caaattgtaa
     3541 taacgccacg caaaccaggc cgcgatcatc actaagagaa ttgttattac ccaaaaaaat
     3601 gaattcatgc ttcatccttc gaatcgatat cccccgcctc gtacggtgac gatccgtgtt
     3661 gggctacccg gatcctcctc caccttttgc ctaagccaac ggacatgcac atccacggtg
     3721 cggctgtcgc cgataaagtc ccagccccag acgcgctcaa gaataaattc gcgtgaaagc
     3781 atttgtcctt tgtgcgcggc gaagaattca agcaactcat attcctttgg tttgagttgc
     3841 agtggaatgt tatgcaatgt gacttcgcga cgcgtgatat tgatcacgag atcattgaag
     3901 gacagtgtct cctgtttctt ctcgggctgt ggcgacttct ctagctcctc cctgagcaga
     3961 cgcgtacggc gtaattgcgc tttgactcgc gccatcagct cacgcatgga aaacggcttg
     4021 gtcagataat catccgcgcc gacttcgaga cccacgacgc ggtcaatttc atcatcacgc
     4081 gcagtgagca tcagaatcgg cgtggacatt tccttgcgga ggatgcgtgc cacttcaaat
     4141 ccatccagtt cggggagcat gatgtccagc aagatcaggt caggctggag acggcgcgcc
     4201 gattcaagcg ccgagcgccc atcacctacg gcttccacat tgaagccgtc tttttttagg
     4261 ttatagatga gggtgtcacg cagcgcgggc tcgtcttcta caacaaggat gttatctggc
     4321 attatgctct gttgccactg gtcacatcaa gaactacact ttcctgataa attcgcaggt
     4381 taaggtccaa atcgtaagtt tagttagtta tgcggcacta cgtaacaaac tataccagat
     4441 tatttttttc gatggttaag gtcttgttaa ataaaaagag ccgccgggtg tagcggctct
     4501 tgcttatatg gttgccttat cttgaaacta caatttgcat gctgctcccc gcacaatcta
     4561 aattatctct tcccttacat gggtccataa ttagattgtt caatagtaaa cagaaaacag
     4621 gcgacagctt acaatctgtc gtctgttttt aaattcagat agtaatgatg ttattatgat
     4681 agtatctgta aaattccagt aatttccatc gtctaaccac catgacccgc gaattctatt
     4741 tctcctggca atgggatcta cgttcctcgc ctgaggcgat ctggccctat gtggcagata
     4801 cgaatcgctt caaccgcgat acgggccagc ctgaagtcga gatgctggat aacaaaatgg
     4861 gcaccaagaa tgcgcggatg aaactccctt tcatcagggt ggaatgggaa gaagagcctt
     4921 tcaagtggac gtatccatat tcgtttgggg ttttgcgaat ttatcggaag gggcccattg
     4981 acgaaatgcg cgtgcaggcg gacttcgagc cgcttgctac tggcggcaca cgcattaagt
     5041 atcagacctg gataaagaca agcaacatcc ttgcacagct tggtattcct tttgggattg
     5101 gcgtcatcgc gaaaaacaga tttgagcggg ctttccgctt ctatgaccgc attgcagaaa
     5161 aagacaaaag tcagaagttg atcagcattg ggcgacctca tggactcaac gcaagagaac
     5221 gcgcccgctt caaatcgttg agtgaaacgg tccttcggca gggcacaagc gcggctttgg
     5281 tagcgcgact ggaatccttc ctcaacgaag ccgatgaact ctccctccag cgaattcgtc
     5341 cctatgcact tgcagatcat tggggtgaga atcgtcgcgc cgtgctggag atgttcctgc
     5401 gagccacgcg tgcgggaata ttcgatatgt cgtgggacat actttgtcca tcttgccgcg
     5461 gtacaacaga agggcgtacc aatctgggtg aggtgcgcag tcagtcacat tgcaatacat
     5521 gtcagatcga tttcacaaca aatttcgatc acaacatcga agtgatcttc cagcccaatc
     5581 catctgtgcg gcccgttgaa tttactgccg cattttgtgt gggcagcccg caattgcagc
     5641 ctcatgttgt gatgtcacag agcatatcat cgctgcgttc gttgcctgtc aatgtaaaac
     5701 ttgagcacgg acgttataca ttgcgtgctt cgaatatagc ggggtcgatc tctttgtttg
     5761 cagatgcggc gggatcaccc agttcagata tccgggtgac atcctttgga tggcccgctg
     5821 aacaacaaca cgtctcactt ctcccgaccc tcaacctgat caatgcgact gactcagatc
     5881 aaaccttcca actggagcgc acagcctgga gtgatcaggc ctctactgca gccgatgtaa
     5941 ccgcgttaca agtcttccgc gatctttttg ccaccgaagt cgtccgccct ggtgaagaga
     6001 tttcgatcgg atctgtcacg ttgatgttca ccgatctacg tgactcgacg cgcctgtatc
     6061 gcaaaattgg ggatgcctct gcgtttggac gcgtacgtga gcatttcgag atattggaaa
     6121 aatcgattgc agaacaaggt ggcgcgattg taaagaccat gggcgatgct gtgatggcga
     6181 ccttccgtca ccccattgac gggctgcgcg ccgtctggaa cgcgcaaaaa caaattgctg
     6241 aacgcggtga gccgatgcta tggctcaaag ttggccttca taaggggcca tgtattgtcg
     6301 tcaatttgaa tgaccggctg gattatttcg gctcgacagt taatatcact gcacgcctgc
     6361 cgggcttctc acagggcggt gagttgatcc tctctgaagc cttttatggt gaccccgaaa
     6421 tacaggactt tctcacacag gacatcaagc ccagtgcgtt gactaaattt acaggtaatg
     6481 tgaaaggatt tgacgaaccg tttgcgatgt ggaaattagc ggtctgatcg tgggtaggtt
     6541 tgaacttggc ccaaatgcgg gtgtcgaaac cgcccgtttc aaccggctac cgattcaata
     6601 agctcggaaa acttcttcaa atctatattg ccaccagaga caattgccac gatcggtccc
     6661 tgcccagctt tcccagtcag cgctgcggca agaggtaaag ctccggcgcc ttcggcgatg
     6721 atacgcgctt tttctgccat gatccgcatg gctcgttttg tctcctccag gctgaccaca
     6781 ataaatccat ctgctaatgg ctttatccgt tcccacatac gcgggaaaag gctcttgcca
     6841 ccggccccat caacgaacga agcctgccac tccttgaaca cttgtggaga gcccatcgaa
     6901 aacgagagcg ctgcgggagc ggctgtctcc ggctcagcgc cccaaagttg aacatctggt
     6961 cgaagggcct tgatcgcgct gccaactccg gtgatcaacc cgcctccgcc aatgccagca
     7021 atgacagtaa ctacgtctgg tgcatcttcc agaatctcca gtcccatcgt cgcatgaccg
     7081 gcgatgaaat tatgatcgtc gaacggatgc acaaacgtcc cttcgacccc gggaaacgaa
     7141 cgtgcgtcga gggccttcca cgcatcatca taagatacta gaacgagatt cgcgccgagt
     7201 gctttcattc gctcaagttt tgagagtggt gctgtctcta tcgcaacaac cgtgcatggc
     7261 acgccagctt ttttggctgc gtacgcgacg ccttgccctg cattccccgc gctgatggtc
     7321 cacacgccgc gcctgcgttc agcttctgat aacatcccca cggcgttggc agcccctcgc
     7381 agtttgtagg cgttgatcgg ctgcaggttt tcaagcttga gtcgaatatc cgggtagcct
     7441 ggccctaact ccaaacggac cagcggcgtt cgcatgatgg tgttcgcaat gcgttcacgc
     7501 gcttctttga tctcatccaa ttcaatcggc cggaccgctt caagtgttgt ggacattatt
     7561 tgttctcctc tgtcttacta tttcaaacat cagcacagac cccgc
//
LOCUS       DASTJP010000784         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1429771, whole genome shotgun sequence.
ACCESSION   DASTJP010000784 DASTJP010000000
VERSION     DASTJP010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429771"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFQ23_23005"
     CDS             <1..222
                     /locus_tag="VFQ23_23005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909535.1"
                     /translation="RAVAPNLWFLAPGVGAQGGELESALRAGLRKDGKGMLIPISRGI
                     SRAESPAKAAAEIRDEILNIKMQLDREDR"
     gene            312..920
                     /gene="pyrE"
                     /locus_tag="VFQ23_23010"
     CDS             312..920
                     /gene="pyrE"
                     /locus_tag="VFQ23_23010"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET9909536.1"
                     /translation="MTQLDIDRSTLADGLLSAGCIKFGEFTLKSGLKSPIYIDLRQII
                     SHPKLLSEVAQAYLPILSNLQFSRIAGLPYAAIPIATAISLAGNYPMIYPRKEAKTYG
                     TKADIEGQYHAGETVVVIDDLATTGGSKFEAIEKLTGVGLVVKDVVVLVDRQSGAKES
                     LEQAGYSMYAVLTISQLLDYWEENARVEKEKIEQTRKFLSGN"
     gene            927..1823
                     /locus_tag="VFQ23_23015"
     CDS             927..1823
                     /locus_tag="VFQ23_23015"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET9909537.1"
                     /translation="MERDLNRFRGCLLGLAVGDAVGTTVEFQPRGSFPPVTDMLGGGP
                     FRLQPGQWTDDTSMALCVATSLIAKNGFDPKDQMDRYWDWYRNGYLSSTGRCFDIGNT
                     VRQALESYKQTENAFSGSTEPNTAGNGSLMRLAPIPMFYFPHLEEIVKFAGESSRTTH
                     GALECIEACQLFSEMLFRALSGMNKDEILFDATVKISSPKIIDISKGRYRQKSIEQIR
                     GSGYAVESLEAALWCFDQTNTFESAILQAANLGDDADTTAAICGQIAGAYYGESGIPL
                     KWLERLHMRAEITELADKLSIE"
     gene            1824..2414
                     /locus_tag="VFQ23_23020"
     CDS             1824..2414
                     /locus_tag="VFQ23_23020"
                     /inference="COORDINATES: protein motif:HMM:NF022167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909538.1"
                     /translation="MLSFDPVFQATQIFTAYDRPWGVCGGWGIDLFLDRVTRPHKDID
                     FAILRRDQLIIQEYLLSRGWTLEKAVRGELIPWRRGEWIDLPVHTIWCKNPNATPDFI
                     ELLFNEVDSTDFRFRRDRSITLPFEKMILLSASGVPLLAPEIVLLYKSVKPDDPSAAA
                     DFRNVLPKLSSASRAWLVVALNKLYTDHIWLQDLAR"
BASE COUNT          626 a          578 c          632 g          636 t
ORIGIN      
        1 cgtgctgtcg cgccaaattt gtggtttctt gctcctggcg ttggcgcgca gggcggagaa
       61 ttggaatcgg ctttaagagc aggcttgcgg aaagatggaa agggcatgtt aatccctatt
      121 tcacgcggca tctcacgcgc ggaaagccct gccaaagctg cggcggaaat ccgcgatgaa
      181 attttgaata tcaaaatgca attggaccgc gaagaccgct aaggtcgcca agaaaataga
      241 attcctttgc gttctcacct gcacttgcgc caggtgcaag tgtcgcgtac tttgcggtaa
      301 aaggatctac tgtgacacaa ctagacattg atcgctcgac cctcgccgat ggactgctct
      361 cagcaggctg catcaaattc ggagaattca ctctcaaatc cgggctcaag tctcccatct
      421 atattgactt gcggcaaatc atttctcacc ccaaacttct ttctgaggtc gcgcaagctt
      481 atttgccgat actctcaaat cttcaatttt ctcgcattgc gggtttgccc tatgctgcta
      541 ttcccattgc aactgccatc agtctcgcgg gaaattaccc catgatctac ccgcgcaagg
      601 aagccaagac atacggaaca aaagcggata tcgaaggcca ataccatgcg ggtgagactg
      661 tcgttgtcat tgacgatctc gccacaaccg gcggaagcaa attcgaagcc atcgaaaaat
      721 tgacaggcgt gggactggtt gtaaaggatg ttgttgtact ggtggatcgt caatcgggcg
      781 caaaagagtc gttggaacag gcgggatact ccatgtacgc ggtattgacg attagtcagt
      841 tgctggatta ctgggaagag aacgcaaggg tggagaagga aaagattgaa cagacgcgga
      901 aatttttatc agggaactag tcgaacgtgg aacgtgatct aaaccgattt cgcggctgtc
      961 ttcttggttt ggcagtgggc gatgctgtgg gaacaaccgt tgagtttcag ccgcgcggat
     1021 catttccacc tgtcaccgat atgctcggtg gtggtccttt ccgtttgcag cccggtcaat
     1081 ggacagatga cacctccatg gcgttgtgtg tagcgacaag tcttattgcg aaaaacggct
     1141 tcgaccccaa agaccaaatg gacaggtatt gggattggta ccggaatggc tacttgagca
     1201 gcacgggtcg atgttttgac attggcaaca cagtccggca ggcattggaa agctacaaac
     1261 agacagagaa tgcgttcagc gggtcaactg aaccaaacac tgcaggaaat ggctcactga
     1321 tgcgtttggc tccgatacca atgttttact tccctcatct cgaagagatt gtgaagtttg
     1381 caggggaaag ttctcgcacg acacatggcg cgttggagtg cattgaagct tgtcagcttt
     1441 tttcagaaat gctttttcgc gcgttatctg ggatgaacaa ggatgaaatc ctatttgacg
     1501 caactgtcaa aatctcatct ccaaaaatta tcgacatttc caaaggaaga tatcggcaaa
     1561 agtccattga gcagattcgt ggatcgggct atgcggtgga aagtttggaa gctgcgctct
     1621 ggtgttttga tcagactaat acatttgaat ctgctatcct tcaagctgca aatcttgggg
     1681 atgacgcgga taccaccgca gcaatctgcg gtcaaattgc tggtgcatat tatggcgaat
     1741 cgggaatccc cttgaaatgg ctcgaacgtt tacatatgcg agctgagatt accgaactgg
     1801 ctgataagct ttccatagaa tgaatgctga gctttgatcc tgtttttcaa gccacacaga
     1861 tattcacagc ttatgatcgt ccctggggag tttgcggcgg ttggggaatc gacctgtttt
     1921 tagatcgtgt cacacgtcca cacaaagata tcgatttcgc tatcctccga agagatcagc
     1981 tgatcatcca ggaatatctt ttatctcgcg gctggactct cgaaaaagca gtccgtggtg
     2041 aactgattcc ctggcggagg ggcgagtgga tcgatctccc ggttcatacg atctggtgta
     2101 aaaatcccaa tgctacacct gattttattg agctgttatt taacgaagtg gactcaacgg
     2161 acttccggtt tcgtcgagat aggtcgatca cactgccctt tgagaaaatg atactgcttt
     2221 cggcatcagg tgtcccactc cttgcacccg agattgtttt attgtacaaa tctgtcaaac
     2281 cagatgaccc atccgctgca gcagatttca ggaatgtttt gcccaagctt tcctctgcat
     2341 cgcgcgcctg gcttgtcgtt gccctgaata aattgtatac agatcatatc tggcttcagg
     2401 acttggccag gtagtggagt acaatgacaa agactatcta aagttgctat ccgaatcacg
     2461 agcgcggccg gc
//
LOCUS       DASTJP010000785        12819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_493690, whole genome shotgun sequence.
ACCESSION   DASTJP010000785 DASTJP010000000
VERSION     DASTJP010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12819
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493690"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFQ23_23025"
     CDS             complement(<1..492)
                     /locus_tag="VFQ23_23025"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909539.1"
                     /translation="MAKLLTLQLIPQSLRNGDTLEIESNGKRSPWEIVGIYSITGNIN
                     PPLLYVNYEYLSRLIHQPGQIFSLRVITSRHDVDFQKSVKSQLQDLYETRGIQVSSTQ
                     LSAEFIEDQKAQTDILVYFMLVMASLIAIVGGLGLMGTMSINVLERTREIGVMRAIGA
                     SNRD"
     gene            complement(705..1394)
                     /locus_tag="VFQ23_23030"
     CDS             complement(705..1394)
                     /locus_tag="VFQ23_23030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909540.1"
                     /translation="MSTITGTLSNEKGVATLPKVRTFVHRAIWALPIWTAMLFLGTLT
                     HQPDPQTDFASFAAYVTTSQFLFSHLVNSIGGAAIGSIGVIGLMLYLQNTKAAGKAIT
                     GMVLTVAGNTLTSSVFGAAAFAQTAMGHAYLAGEKNALDFYNLVYSAPLFATVLLGLL
                     LFIIGGVSTARAISASGQFPRWIGWVYAITVIGFVLSNFTIPAGQTPMSILLFASAVA
                     VAWTVSRTDTI"
     gene            complement(1410..2222)
                     /locus_tag="VFQ23_23035"
     CDS             complement(1410..2222)
                     /locus_tag="VFQ23_23035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9909541.1"
                     /translation="MNWIKRNDLAVFLLLAFALSWWPWPFNLMNPNSAPMIPWGPMFA
                     AFIVLGLTRGWSGVKSLLADMFRWRVGARWYVLAFLLPIGVTFVAVYLNVALGGPAPA
                     TSVFAVNELILLIPALMFTTLLAGPFTEEPAWRGFFLPRLQTKYSPLAASLILGVIWW
                     SWHMPVMISDPTGQRPPLQYFLSIVAYAVIYTWIYNNTKASVFLVTLMHGVTNTIAAF
                     LFRGLFGEYYGQLWWLYAGIWWVVALFVVVRSNAMVQASNSVSISQNQKATI"
     gene            complement(2254..3807)
                     /locus_tag="VFQ23_23040"
     CDS             complement(2254..3807)
                     /locus_tag="VFQ23_23040"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9909542.1"
                     /translation="MDKDRKGPQRIIIGLLVLILLFALIPETSASAINTTNVEQIDAF
                     VIHQMERHGIPGLALALVDGDKVIFIKGYGKADQTGRLITPQTPFILASVSKPLTAVA
                     IMQLVDTGQVELDTPVQKYLPEFRVADPIVSSQITVRHLLLHTSGIPATACDTKVNAQ
                     TIEEYVAELQTVKLDAPVGSHHNYCSGNYNVLGRIIEVVSGQSFGEYMQEHIFAPLDM
                     QQSFTSEQEAKQAGLAQGYQWLFGLSVPTHHPYNPSQLPSGYMISSVEDMSHFLISQL
                     NDGRYANANLLSAKNVAAMQMAGTNRSEDGGYGFGWVIAPVGEVPAIWHDGVNVNFHS
                     LLLMEPETGRGAVVLMNSFGIVPYQGAYKEIETGVARMLADLDPAPESTRLRTIYLMI
                     DTLLAGLLAIVLFPLLRMGSWHRWLLERQQENRLPLIRVSLRAAWEINFALAFLIGLR
                     VIIVTGLGAQSWYEVLTVFPDFVLWIWVFALTVLLTGVIRMKLILQTRKFAINGSEVA
                     TDAPLIKGM"
     gene            3959..6553
                     /locus_tag="VFQ23_23045"
     CDS             3959..6553
                     /locus_tag="VFQ23_23045"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9909543.1"
                     /translation="MTEYSFGTWVKRRRRALDLTQQGLAQRVGCSVSAIVKIEADERR
                     PSHQVAELLAQTLEIPLDQRDSFLKVARQEKTVDTLGEQPDLSQHSTRPQANIPLPPN
                     ALIGREFELTEINLLIEDPKCRLLTLTGAGGIGKTRLALETASQKQSELELGAVFINL
                     TPLGSRDQIVTAIADALGIVLYNASDRSAQLITRLRDKEVLIVLDNFEHLLSHADCLA
                     LPRDLLAGAPLVKLLVTSREPLQLQSEWAFEVRGFPVPGINESHSPESSSAVKLFVQR
                     ARQTRSGFELRPEDHSAVREICQLVDGMPLAIELAAAWTRTLTCAEIAREIQSNVNFL
                     RTSSRDTTERHRSIRATFEHSWKLLSSEEQTVLRRMSIFKGGFTREAAEYVTGADLSL
                     LSSLVSKSLVHRTEQGHYDLHELVRQYSLEYLKKNESEHIETQDRHSEYYSALLKRRG
                     DAFKSADQPGVSRELASQIANLRQAWRWAGERKQALQVGQAADTLFWLYESRCDCREG
                     VPLFGHVATHIGSESISLEETVPNETRAITQARVLAYQGFFCLRQGQHPQSKDLLQRS
                     LGILHPMAEGGSLEARDALSNTLAFLGMLTVSLGDYTNGNRYLNEGLEIKRTIKDGWG
                     IAFCLRQLGVLGFYQGAYDEADRLLNEGLEVSRALGNSWAIAYSLDFLSTAAYARGAY
                     GEAEKLLREGLMLSQQVGDRFTTAYALNGLGLVKQSLGAHDEARQLLEDSISIWREIG
                     DLGSMAQSLNNLGNVFLVMDKHLEAQNCFRDALAVAKHAGLIPVMLDSMMGEAEIRAA
                     NGEIKSAFEAAWMVSQHPSSSHATRTRAGMMCSSIEKQLSPDEVASIRAKNSTIESSA
                     QEILSQVA"
     gene            complement(6566..6940)
                     /locus_tag="VFQ23_23050"
     CDS             complement(6566..6940)
                     /locus_tag="VFQ23_23050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909544.1"
                     /translation="MESSSITPSHKTGAAWQLLGDFDLTQDPQVDTIRAWLEELLTPL
                     NLQDELAHQLLGSAQDAAIRALNSDNGTDFERIHLSVFAPKERDVQCNTWGFFRIEKI
                     NSTEQSEDHPQFIVEFYIYLEG"
     gene            complement(7046..8338)
                     /locus_tag="VFQ23_23055"
     CDS             complement(7046..8338)
                     /locus_tag="VFQ23_23055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9909545.1"
                     /translation="MNLTRNSIQTAIPSVSLQGTGLIVLFAFVHTISDAVTNMLSALL
                     PTLQNRFGLTGTTLAMLVATLSLSALMTQPLFGALADRVGHRRIAALGVIFNAILFSL
                     IGIVSDVKTLFVLILIGGLGSAALHPAIASMARKAGGNKPEVAVGLFSAGGTLGIAIG
                     PIIIMILLANLGLSFTPWLMIPGILLGTCMFFFVPEDDQRHTDASNRIFDLRLLSGRL
                     GLLALIGMLGNLAFVTFMCAMPLWLVHTHGYSSDAALIGWTLSTFSLSAAAGGIVGGA
                     LSNWLGAKRLIVGTLLLAVVPLYSILILTPGTIAYFGAVALAGALVNAGMPMLIVTAQ
                     DLAPKAAATASGMLMGFSAGLAGLAYVLIGGLQEILGLAPAMAIGYLSLIAGAVLAMV
                     AIQPRSVDEEKPIEKLSCLCSTCMDQNIAAYPQRMNNS"
     gene            complement(8482..9345)
                     /locus_tag="VFQ23_23060"
     CDS             complement(8482..9345)
                     /locus_tag="VFQ23_23060"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HET9909546.1"
                     /translation="MNPKYPFQFILAALIIASLSCSFANQSRFRKLEIGPLNTVTLSE
                     PYPGTSAIQDVTLSMAVGELTLSGGAETLIEGKMQYNVEGWKPNIEYKNNALTISQEA
                     EFPVNGFPGDDVVNIWDLKLGQTPMNLVLTADAYDATLNLSGLPIHSMVVQDGASNSV
                     IRFDTLNPAEMQELTYQTGASEITFLGLAHANFAQMSFDGGVGEYTFDFAGDLQRDAV
                     VNIYAGLSNVRIIVPEGMAARVAVDDGLRKIYATGAWKVEGGKYANDGAGPLLTIVVD
                     MGAGELHLMNP"
     gene            complement(9395..10291)
                     /locus_tag="VFQ23_23065"
     CDS             complement(9395..10291)
                     /locus_tag="VFQ23_23065"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9909547.1"
                     /translation="MTTIAGTLSTEKGISVLIRRHPLVAYFFLAYAGMWIVISPLVMD
                     SFGWIELSDGISLLLFILSSLSGPTVAAYWVTGVLEGKAGMGRLLRRTFQLRAGLQWY
                     AVALFIFLSVWLIAYSFLYNGAPLAALAENPSLLLSVFLPSVIMGLLIPSIGEEPGWR
                     GFALPRLQAAYGPVIGTLILGTLHGVWHLPALFTPLLGPFTVEGFIVFVLTAAAGTFI
                     YTWLFNNTRGSVWMAMVLHASSNAASQLVSSMIPKEVELTGWMKILESGWLNVIVFGA
                     VALLLTISTRGTLGYRPDQADH"
     gene            complement(10675..11502)
                     /locus_tag="VFQ23_23070"
     CDS             complement(10675..11502)
                     /locus_tag="VFQ23_23070"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9909548.1"
                     /translation="MNIPILPLNNGLSIPQIGLGTSPLNDEAIAPLVVAAIEAGYRHI
                     DTAYRYNNEKGVGKGIRDSGIDREDLFLTTKLDGEFQGNDRAITGLDESLRRLGVEYV
                     DLLLIHWPLPKRDEYISTWKTFEKLVAAGKTRSIGLSNFKPSHIDRLLAECTIRPTVN
                     QIQLNPRITRTDQVAYNSQHDIVTEAWSPLGAGNDLLKAPTLVSIAKKYEKTPGQVVL
                     RWQIDLGIVTIPRSSNPARIAENLNIFDFALTNDEIASISALDTGKEPRVDSDKSGH"
     gene            complement(11568..>12819)
                     /locus_tag="VFQ23_23075"
     CDS             complement(11568..>12819)
                     /locus_tag="VFQ23_23075"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET9909549.1"
                     /translation="PVTNAAAPTVGFTPQIRLGFDVGDQWEPAIAADRFGHVYMLYPQ
                     YEGVPGCANCSNPTQILQISNDRGQTWGSPFVLYPAGATTGGQWDPQVVVDPVDGRTV
                     YTTFMQNNKSDIIVGKSTDFGLTWSFVTSDATNAGTDKPILTVRGQDVYVVYTHAQTT
                     YAAYSHNGGASFTEVKVSKGGRLGWALAGGGTVTPNGDVFFAWAGYEGNGGATGNVNL
                     YVSKSTNGGANWTSKVIDISSSPPQCPEFNCGWAFLGAQLVMTSDSNGTIYLLWNAGS
                     TATGPERIYFAKSTDGGNNYSTKVEVSTAPAGTHHAFPAIAAVGNGDVRISWMDARAA
                     NGGLDRWNVYYRSSTNGGSTWSSEVDLSTAVSGIPYIFNDGFRFPFGDYYELDIDDQG
                     TNHVIFGIGFDYNAPGSVWYVKGK"
BASE COUNT         3247 a         3213 c         3318 g         3041 t
ORIGIN      
        1 atctctattc gatgcaccaa tggcgcgcat cacgccgatc tcgcgtgttc gctccagtac
       61 gttgatactc atcgtaccca tcaggccaag cccgcccacg atagcaatca gactcgccat
      121 gaccagcatg aaatagacca ggatatctgt ctgcgccttt tgatcctcaa taaattcggc
      181 gctcagttgt gttgaactga cctggatgcc gcgggtttcg tataaatcct gcaattgaga
      241 tttcacggat ttttgaaaat caacatcgtg cctggacgtg atcactcgca acgaaaaaat
      301 ctgaccgggc tgatgaatga ggcggctgag atattcataa ttcacataca gcaggggagg
      361 gttgatgttg cctgtaatgc tgtaaatccc tacgatttcc cagggggacc ttttgccgtt
      421 tgactctatc tccaaagtat ccccattacg taacgactgg gggatgagtt gcaatgtcaa
      481 caatttcgcc actaaaataa ttatccgact agatgcttca ttgaatttgt gtgtttacag
      541 tttgtttttc tggaaaagtt tatataaata attttttcaa aagattaggc acgagcaata
      601 taatttgatt atcgtcacaa atttttagaa ttccaaacct tgtgaattat cctccccgta
      661 gccaatgctc gtggggagga taattttata aacagactgg ccgattagat tgtgtccgtt
      721 cggcttacgg tccatgcaac tgcaacggct gacgcaaaga gcaatataga cattggggtc
      781 tgcccagctg gaatcgtgaa gttgctaagt acaaacccga ttaccgttat ggcgtacacc
      841 catccaatcc atcgcgggaa ttgtccggag gcagagatgg ctcttgcggt agacacacct
      901 ccaatgatga aaagtaagag tcctaaaaga accgtggcga agagtggagc agagtagacg
      961 aggttgtaga agtcgagagc gtttttctct cccgcgagat aggcgtgccc catagcggtc
     1021 tgtgcgaaag cagccgcacc aaataccgag gaagtgagtg tatttcctgc tacggtgagc
     1081 accattccag tgattgcttt tcctgcagcc ttggtgtttt gcaaatagag catcaatccg
     1141 ataactccaa tagacccgat cgcagcgccg ccgatgctat tcaccaaatg ggagaacaaa
     1201 aactggctgg tcgtgacgta tgccgcaaag ctggcaaagt cagtttgtgg gtctggctga
     1261 tgggtgagag tgcccaaaaa cagcatggca gtccatattg gaagagccca aatggctcta
     1321 tgtacaaatg tacgtacctt gggtagagtg gccacacctt tttcatttga taaagtacca
     1381 gttatagtag acatgatatt ttctcctttt tatattgttg ctttttgatt ttgacttatt
     1441 gacactgaat tgcttgcctg caccattgca tttgacctga ccactacaaa gagagctaca
     1501 acccaccaaa taccagcata gagccaccat aattggccat aatattctcc aaatagacct
     1561 cgaaatagaa acgcagcaat cgtattcgta actccgtgca taagtgtcac aaggaacacg
     1621 cttgcttttg tattgttata gatccatgtg tagatgactg cgtacgctac tatgcttaga
     1681 aaatattgga gcggaggtcg ttggccggtt gggtcgctga tcattactgg catgtgccaa
     1741 ctccaccaga ttaccccgag gatcaagctg gctgccaaag gtgaatattt tgtttgcagc
     1801 ctcggcagga aaaatccgcg ccaggctggt tcttcagtga aagggccggc aaggagggtc
     1861 gtgaacatca gcgcaggtat tagcagaatt aactcgttga ctgcaaacac agaagtggct
     1921 ggcgctggtc cgcccagagc gacattaaga taaaccgcca caaaggtgac tccaatcgga
     1981 agaagaaaag cgagcacata ccatcgtgca ccaacccgcc aacggaacat gtctgccagt
     2041 aaacttttca cgccggacca gccacgcgtc aggccaagaa cgatgaaagc cgcgaacatc
     2101 ggtccccagg gaatcattgg agcgctgttc ggattcatca agttgaatgg ccaaggccac
     2161 cacgagagtg caaatgcaag taataaaaat actgccaggt cgttacgttt tatccaattc
     2221 acaatattca tcctttctaa aatgagtttg gggttacata cctttaatta atggagcatc
     2281 tgtggccacc tcgcttccat tgatagcgaa tttccgcgtt tgtagaatca gcttcatgcg
     2341 gatgacacct gtcaacaaca cagtcagcgc aaatacccag atccacaaca caaagtcagg
     2401 gaaaacggtc agcacttcat accatgattg agcgccgagg cccgtgacga tgatcacgcg
     2461 tagaccaatc aggaaggcca gtgcaaagtt gatctcccaa gccgcccgca agctgactct
     2521 gatcaaaggc agacgattct cctgttgcct ttccaatagc cagcggtgcc agctgcccat
     2581 ccgcagaagt ggaaatagca cgatggcaag gagtcccgcc agcagggtat cgatcatcaa
     2641 atagatagtc ctcagcctgg ttgactcagg cgcggggtca agatcggcga gcatccgcgc
     2701 gacgcctgtt tcgatttcct tgtatgcacc ttgataaggg acaatgccaa atgaattcat
     2761 caacacgacc gcgccacgcc cggtctcagg ttccatgagc agcagcgaat ggaaattgac
     2821 attgacgcca tcgtgccaga tggcgggcac ctcacccaca ggtgcgatga cccaaccaaa
     2881 tccatacccg ccatcttcgc tacggttcgt acccgccatc tgcatggccg caacgttttt
     2941 tgcggacagc aagtttgcat tggcatatcg accatcattg agttgcgaga tgaggaagtg
     3001 actcatgtcc tcgacactcg agatcatata tcccgaagga agctgcgaag gattataggg
     3061 atggtgcgtc ggaacagaca atccaaacaa ccactgatat ccttgggcca gcccagcctg
     3121 cttcgcttcc tgctcggagg taaaactctg ctgcatatca agcggagcaa aaatatgttc
     3181 ctgcatatat tcaccaaagg attgcccgga gacaacttcg atgatgcggc cgagtacgtt
     3241 gtaattgccg ctgcaatagt tatggtgtga gccgaccggt gcatcgagtt ttacagtttg
     3301 cagttcggcc acatattctt caatggtttg tgcgtttact tttgtgtcgc aggctgtggc
     3361 agggattccg cttgtatgga gcaagagatg gcgcaccgtg atctggctcg atacaatcgg
     3421 gtctgcaact cgaaactctg gcaaatactt ttgcacgggt gtgtcaagct caacttgacc
     3481 tgtatcaacc aattgcataa ttgccacggc agtcaacggc ttgctcaccg acgccaaaat
     3541 aaatggcgtt tgcggagtga tgagacgacc cgtttggtcg gcctttccat aaccttttat
     3601 aaagatcact ttgtcaccgt cgaccaacgc caatgccagg ccggggatgc cgtgacgttc
     3661 catctggtga attacaaagg catcgatctg ttccacattc gtggtgttga tggcagatgc
     3721 agacgtttca ggtattaacg caaataacaa tatcaatacc aacaaaccga ttatgattct
     3781 ctgtggacct ttccgatctt tatccatatt tcttctcctt ttcattacaa ataaaattaa
     3841 tgcctcccag tttatacatg agagagaata tggattggta cgaattaaga tacaataatg
     3901 tagacaaaaa atagtacaat ttgataatcg tttcgactat ttgcctggtg gagtgattgt
     3961 gacggaatat tctttcggaa cctgggtcaa gcgcagacgg agagccctcg atctaacaca
     4021 gcagggactt gcccagcgcg tgggttgttc tgtctctgct atcgtgaaga tcgaagcgga
     4081 cgaacgccgc ccgtcacatc aagttgcgga attactggcg cagactctgg agattccgct
     4141 cgaccagcgc gactcatttc tgaaagtggc ccggcaggaa aaaaccgttg acacattggg
     4201 ggaacaacca gacttatccc agcactcaac tcgccctcag gcaaatatcc cgctcccgcc
     4261 caatgccttg atcggacgtg agtttgaact tacagagatc aatcttctca tcgaagatcc
     4321 caaatgccgt ttgttgaccc tgaccggagc aggcggaatc gggaagacac gccttgccct
     4381 ggaaacggct tcccaaaagc aatctgagct tgaactcggg gcagtcttta taaaccttac
     4441 gcctctgggg agtagagatc agatcgtgac cgcaattgca gatgcactgg ggattgtgtt
     4501 gtataacgct tctgatcgct cggcccagct gattactcgt ttgcgtgata aggaagtgct
     4561 gattgtgctc gataacttcg agcatcttct tagccacgca gattgcctgg ctttgcctcg
     4621 cgacctttta gcgggcgcgc cactcgttaa gctgttggta acgtctcggg agccgctgca
     4681 attgcagtcg gaatgggcat ttgaagtgcg tgggtttccc gttccgggta tcaatgaatc
     4741 gcattccccg gaatccagta gtgcggtgaa gttgtttgtg cagcgtgccc ggcaaaccag
     4801 gtcaggtttt gaattacgac ctgaagatca ttccgcagtt cgggagattt gccaactcgt
     4861 ggatggtatg ccgttggcga ttgagttggc agcagcgtgg acacgaactc tcacttgcgc
     4921 agagatcgca cgcgagatcc aatccaatgt caatttcctg agaacctcct cacgggatac
     4981 tacagaacgt caccgctcca ttcgtgcgac attcgagcat tcgtggaagc tgctttcctc
     5041 agaggaacaa actgttctca ggcggatgtc cattttcaag ggcggtttca cgcgtgaggc
     5101 ggccgaatat gtgaccggcg cagacttatc cttattatcg tcgttggtat caaaatctct
     5161 cgtgcatcgc accgagcagg gacactacga tctgcacgaa ctggtgcgcc agtattcact
     5221 tgaatatcta aagaagaatg agtctgagca tattgaaacg caggatcgtc acagcgaata
     5281 ttattctgct ttactgaaac gccgtggcga tgcgttcaag agtgctgacc agccaggtgt
     5341 gagtcgtgaa ctcgcttccc agatcgcgaa tttgcgtcag gcatggcgtt gggccggcga
     5401 gcggaagcag gccctccagg ttggacaagc cgcagatacc ctcttctggt tgtacgagtc
     5461 gcgttgtgac tgtcgcgagg gggttccgct tttcgggcat gttgcaactc acattggatc
     5521 tgaaagtatc tctcttgagg aaacagtgcc gaatgaaacg cgtgccatta cacaggcacg
     5581 cgttttggcg tatcagggat tcttctgtct gcggcagggc cagcatccgc aatcgaagga
     5641 tttgttgcag cgtagtctag ggatattgca tccgatggca gagggcggtt ctctggaagc
     5701 ccgagacgca ttgtcgaaca cactggcatt cctggggatg ctgaccgtct ctctcggcga
     5761 ttacacaaac ggcaaccgtt atttgaacga aggactggaa atcaagcgca cgatcaagga
     5821 tggctgggga atcgcgtttt gtctgcggca attgggcgta ttgggttttt atcagggcgc
     5881 gtacgatgag gcagatcgtt tgctaaacga aggtctggag gtcagccgcg ctctcggtaa
     5941 ctcgtgggcc attgcctatt cgttggattt tctcagcacc gcagcctacg cgcgaggtgc
     6001 atatggcgaa gcagaaaaac ttctgcgcga gggactgatg ttaagccagc aggttgggga
     6061 tcgatttaca actgcatacg ccctcaatgg actgggcctg gtgaaacaaa gtttgggtgc
     6121 acacgacgag gcgcggcagt tattggaaga cagcatctcg atctggcgcg agatcggtga
     6181 tctgggcagc atggcccaat ccttgaataa tttaggcaat gtttttctgg taatggacaa
     6241 acatctcgaa gcccaaaatt gcttccgtga tgcgctggcg gtcgcaaaac acgccggtct
     6301 gatccccgtg atgttggatt cgatgatggg cgaggctgag attcgcgcgg cgaacggcga
     6361 aatcaagtcc gcgtttgaag cggcatggat ggtcagccaa catccatcca gttctcacgc
     6421 aactcgaacg cgcgcaggaa tgatgtgctc cagcatcgaa aagcaactct cgcccgatga
     6481 ggtagcctcc atccgggcta agaacagcac tatcgaatcg agcgctcagg aaattctttc
     6541 ccaggtagcg taactccagg ggatattatc cttccaaata aatatagaac tccacgatga
     6601 attgagggtg gtcttcgctc tgttcggtgc tgttgatttt ctcgatgcga aagaaacccc
     6661 acgtattgca ctgcacgtcg cgctccttcg gtgcgaaaac agaaaggtga atgcgctcaa
     6721 agtccgttcc attatctgaa ttcagcgccc gtatcgccgc gtcctgagct gaacccaata
     6781 gctggtgtgc caactcatcc tgcaggttga gcggagtgag tagctcctct aaccaggcgc
     6841 ggatcgtatc cacctgtggg tcctgggtga gatcgaaatc ccctaacaac tgccaggctg
     6901 cacctgtttt atgagaaggg gtaatagagg aggattccat tcagcgtacc aatgcctatc
     6961 tatcataaaa attcttgact ggttgacgag attttcgtgg acgatgccga tcactgtcaa
     7021 tgcgactttg ttaaatattg ttgggttaag aattgttcat tcgctgtgga tacgcagcga
     7081 tattttggtc catgcaagtt gagcacaggc aggagagttt ttctatgggt ttctcctcgt
     7141 ctaccgagcg cggctggatg gcgaccattg ccaggactgc ccctgcgata agcgagagat
     7201 aaccgatcgc catggcgggt gccaggccaa gaatctcctg caggcctcct attaacacat
     7261 aggccaggcc agcaagcccg gcggaaaagc ccatcaacat gccggaagcc gtcgccgcgg
     7321 ctttgggcgc caggtcctgc gcagtgacga tcaacatcgg catgcccgcg ttgactagcg
     7381 cacccgccaa cgcgaccgcg ccgaaatacg caattgttcc tggcgtaaga atgaggatgg
     7441 aatagagtgg cacgacggca agaagtagag tgccgacaat caaacgtttc gcgccaagcc
     7501 agttgctgag cgctccaccc acaatgcctc cggctgcagc ggacagcgaa aatgtagata
     7561 gagtccaacc gatcaaagcg gcgtcactgc tatatccgtg ggtgtggaca agccataatg
     7621 gcatggcgca cataaaggtg acaaacgcca ggttgccaag catgcctatg agagccagca
     7681 ggcccagccg acctgacagg aggcgtagat cgaagatcct gttcgaggca tcggtgtggc
     7741 gttgatcatc ttctgggacg aagaagaaca tgcaggtacc cagcaaaata ccagggatca
     7801 tgagccatgg cgtaaaggat aggccgaggt tcgccagcag gatcatgatg atgataggac
     7861 cgattgcaat gcctaacgtg ccacctgcgc taaacaatcc aacagcgact tccggtttgt
     7921 tccctcctgc tttgcgcgcc atactggcga tggcaggatg caacgcggca gaccccaacc
     7981 caccgatcag gatcagcaca aagagagtct taacgtctga gacgatgcca atcaggctga
     8041 acaggatggc gttgaatatg acacccaacg cggcaatgcg acgatgaccc acgcgatctg
     8101 ccagcgcgcc aaacaagggc tgggtcataa gcgcactcag tgaaagcgtc gcgaccagca
     8161 ttgccaatgt agttccggtc agcccaaagc ggttttgaag cgttggcaat aacgcggaaa
     8221 gcatattggt cactgcatca ctgatggtgt gtacgaaggc gaacagaacg atgagtccgg
     8281 tgccttgcag tgaaacagac gggatagcgg tttgaattga gtttcgggtg aggttcatga
     8341 tgatttactc gcgattttga ttaatgtatg gatctctgca attactacgt cggggcggtc
     8401 aaaggtagag aaggcagaga tggtaaagac gcagtattca tagaagtttc ctgtttgatg
     8461 aggtgtttct ttgccagaag cctatgggtt catgagatgt aattcaccgg cgcccatatc
     8521 tacaacaatg gttagcaacg gtcctgcgcc gtcgttggca tacttgccgc cttcaacctt
     8581 ccaggcgccg gtggcataaa tctttctcag tccatcatcg acagcgaccc gggcggccat
     8641 gccttccgga acaatgatgc gtacgttgct caagccggcg tatatgttga ccaccgcatc
     8701 tcgttgcagg tcccctgcga aatcgaacgt gtactcaccc actcctccgt caaaggacat
     8761 ttgagcgaag ttcgcatgcg ccagacccag gaaggtaatc tcggatgcac cagtttgata
     8821 cgttagttcc tgcatttccg ccggattcaa agtatcgaaa cggatcaccg agttgcttgc
     8881 gccatcctga acgaccatac tgtggatagg caatccgctc agattaaggg tcgcatcata
     8941 agcgtcggcc gtcagcacca gattcatagg ggtttgcccc agcttgagat cccagatatt
     9001 gacaacatcg tctcccggaa agccattcac cgggaactcc gcttcttgcg aaatggtcaa
     9061 tgcgttattt ttgtattcga tgttcggttt ccaaccctcg acgttgtatt gcattttgcc
     9121 ttcgatcaac gtttcggctc cgccggagag ggtgagctca ccaactgcca tcgacaatgt
     9181 cacgtcctga atggcacttg tgcctggata aggctcgctc agagtgacgg tgttcagcgg
     9241 accgatttcg agtttgcgaa atctcgactg attcgcgaat gagcaactca gagaagcaat
     9301 gatgagtgcc gccagaataa actgaaatgg atattttgga ttcatgctat cacctgcatt
     9361 gaaaaagatt gaagttttca aaatgtgaat aattctagtg atctgcctga tcgggtcgat
     9421 atcccagcgt gccgcgtgtg gagatagtta acagcaaggc gacagcgccg aagacgatca
     9481 cgttgagcca gccagactcg aggatcttca tccagccggt caactccacc tctttcggga
     9541 tcattgaact gaccagttga gatgcggcgt tactcgaagc gtgcaagacc atcgccatcc
     9601 acacactgcc gcgggtgttg ttgaaaagcc acgtataaat aaaggtcccg gccgcggcgg
     9661 tcaacacgaa aacgatgaat ccttcgacgg tgaatggacc aagtaaaggt gtgaacagcg
     9721 caggcagatg ccagactcca tgaagggtgc caaggataag cgtcccgatg acaggaccat
     9781 atgccgcttg caaacgcggt aacgcaaaac cgcgccagcc gggttcctca ccgatggagg
     9841 ggatcaggag acccatgatc acactgggca ggaaaaccga gaggagcagg gacgggttct
     9901 cagcaagtgc cgctagcggc gcgccgttgt agaggaaact gtaggcaatc agccaaacgc
     9961 tgagaaaaat aaacagggcg acggcatacc actgcaagcc agcccgcagc tggaaggtcc
    10021 ggcggaggag acgtcccatg ccggctttac cctccaatac gccagtaacc cagtaggcgg
    10081 caacagtcgg cccactcaag gaacttagaa tgaacaggag gaggctgatc ccgtccgaca
    10141 gttcgatcca tccgaagcta tccattacca atggcgagat aacgatccac atccccgcgt
    10201 aggcgaggaa gaagtaggca accaggggat ggcggcggat gagaaccgaa atcccttttt
    10261 cagtggatag agtaccggca attgtagtca ttatgttttc tccttttttt tgatttagaa
    10321 ccaccgaaaa cacgagctat gctttacaac agaatgtagt aagtctgctg agatccatat
    10381 tgttcttagc caggtctgta cagcttctcc tggcttgccc ataatggtta gtagggcgga
    10441 tgatgatgtt ataaagaaaa agcccggcct tatggtgtag aaaattccca caagaacatg
    10501 tacaaaaagt agtacatgcg tgttaaaaaa ataaggcacc gagaggtgcc ttttcaagat
    10561 gagaatagga tttgagaaac tgaaatcggt ctacgatgcc aattgagcta ctgtggaatt
    10621 gggattatca aggaagggtg tgtcattttc gtgctgacga ttattttgct tgcatcaatg
    10681 cccggattta tcagaatcaa ctcttggctc cttgccagta tcgagtgctg agattgacgc
    10741 aatttcatcg ttggttagcg caaagtcaaa gatgttgagg ttctccgcaa ttcttgcggg
    10801 gttcgaggac cttggaatcg tcacgatgcc caggtcaatc tgccagcgaa gaaccacttg
    10861 accgggagtc ttttcatact tctttgcaat actcactaaa gtgggagctt tcaataaatc
    10921 gtttccggcg cccagtggac tccatgcttc ggtcacgata tcgtgttgac tgttgtaggc
    10981 gacctggtct gttcgtgtaa tgcgcggatt caactgaatc tgattgaccg ttggtcggat
    11041 ggtacattca gccaggagtc gatcgatgtg agatggcttg aagttcgaaa gcccaatcga
    11101 gcgcgtcttt cccgcggcga ccagcttttc gaaagtcttc caggtcgaga tgtattcgtc
    11161 acgctttggc aacggccagt gaatcaggag caagtccacg tactcgactc ccaaacgccg
    11221 aagcgattca tccaacccgg tgatggcgcg gtcattcccc tgaaattcgc catccagttt
    11281 tgtcgtgagg aagagatctt cgcggtcaat gccgctatca cgaattccct tgccgacccc
    11341 tttttcattg ttatatctgt aggcggtatc gatatgccgg tagccggctt caattgctgc
    11401 gacgaccagt ggcgcgatgg cctcatcgtt gagcggtgat gtgccgagtc cgatctgagg
    11461 aatggaaaga ccgttattaa gtgggagaat gggaatgttc ataacttgaa ttgtaacgga
    11521 tttacttgga agcctccgat ggtacgaacc catcggaggc cttcatctta ttttcccttt
    11581 acgtaccaaa ctgagccggg cgcgttatag tcgaatccga ttccaaatat cacatggttt
    11641 gtgccttggt catcaatgtc cagttcatag taatcgccaa acgggaagcg gaacccatca
    11701 ttgaaaatat aaggaatgcc gcttacagct gtagagaggt caacttcgct gctccatgtg
    11761 gaaccgccat tggttgaact gcgataataa acattccatc gatcaagtcc accattcgcg
    11821 gcacgcgcat ccatccatga aatgcgaaca tcaccatttc ctacagcagc aatggcaggg
    11881 aacgcgtgat gagttccagc cggcgccgtt gagacctcta cttttgtaga atagttattc
    11941 ccaccatcgg tggacttggc gaagtaaatc cgttcaggtc ccgttgcagt tgaccctgca
    12001 ttccaaagca aatagattgt gccgttggag tcagaagtca tcaccaactg agcacccaga
    12061 aatgcccaac cgcagttgaa ctccgggcat tgcggagggg aggaagatat gtcgatcact
    12121 ttggaggtcc agttggcgcc accgtttgtc gatttgctca catacaaatt tacattgccg
    12181 gttgcgccgc cgttcccttc atagccggcc caggcgaaaa atacgtctcc attcggagtg
    12241 acggttccac cgccggcaag tgcccagccg agtctgccgc ctttactcac tttgacttcc
    12301 gtgaatgaag caccaccatt atgagaatag gcagcatatg tcgtttgcgc gtgtgtataa
    12361 acgacgtaga catcctgacc gcggactgtc aaaatcggtt tgtctgtacc tgcattggtg
    12421 gcatccgatg tgacaaacga ccacgtcagg ccaaaatcag tggactttcc cacgatgatg
    12481 tcacttttat tgttctgcat gaacgttgtg taaacggttc ggccatctac cgggtctacc
    12541 acgacttgcg gatcccattg cccgcccgtt gtcgcgcctg ccggatagag tacgaaaggc
    12601 gagccccagg tctgccctcg atcattactg atctgcagga tctgagtggg gtttgaacag
    12661 ttcgcgcacc cagggacacc ttcatattgc ggatacaaca tataaacgtg tccgaatcgg
    12721 tctgcagcaa tggctggttc ccactgatca cccacatcaa aaccaagccg gatctgtggc
    12781 gtgaaaccaa ctgtgggcgc tgccgcgttt gtcaccggg
//
LOCUS       DASTJP010000786         3834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_71195, whole genome shotgun sequence.
ACCESSION   DASTJP010000786 DASTJP010000000
VERSION     DASTJP010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71195"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="menC"
                     /locus_tag="VFQ23_23080"
     CDS             complement(<1..898)
                     /gene="menC"
                     /locus_tag="VFQ23_23080"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET9909550.1"
                     /translation="MKLSSLTLHHISMPLVAPFETSFGRETVRECILIVAQSEGLVGY
                     GECVASRDPGYSYETTGTAWHILKDFIAPLILGKDINDAEEFQSHVEGIRGHHLAKAA
                     VEMALWDLLGKRDGKSLKEMFGGTRDKVDVGVSIGIQESASALVSTVASYLEKGYRRV
                     KIKIKPGREVDETSAVRNAYPDLRLQVDANSAYTLESASALKPLDDLNLLLIEQPLFE
                     DDIWDHRRLQAEFKTPICLDESVVSPRHARYALEMEACKIINIKPARVGGLSQAIAIH
                     DFCVGRNVPVWCGGMLETGVGRA"
     gene            1115..3442
                     /locus_tag="VFQ23_23085"
     CDS             1115..3442
                     /locus_tag="VFQ23_23085"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HET9909551.1"
                     /translation="MQALLDDANGLYVTDDTPNAEPRYRARFYLDPNSLTMANNDAFF
                     LFRGFQGTSTDILRVELRYSSSAYQIRAALMDDASTWVNTNWFNLTDAAHYMEVDWKA
                     ATSAGANNGYLTLWIDGTQSADLTSVDNDTWSIDRARLGALTGIDAGTSGTFYLDAFE
                     SRRSSYIGPMAFGGGHFTSYHYVPLQQGNSYTGQPDATDGIDTYLLNTSPTTNNGTAV
                     TMWIGESNNATDKVARSLIKFDLSSIPSNATITSATLSLWTDADFSDNDRTIRVYRLK
                     VPFSETQATWNEASTGVNWQSPGASGTNDRESTDIGWVLIPENQALDVEKQIPLSTAQ
                     IQEMINGTFTNNGFIIIADTELNDRFSYKTSDASAGTKRPKLVIEYTTSSSTPTNTPT
                     PGPSPTATRTQTPGASPTPTPTQPPQVAFNSATFTYDGDGKRVKSTFNGATTTYFVGN
                     HYEVTGSTVIKYYYAGAQRIAMRTNGTLNFLLGDHLGSTSLITDSTGVKTNEQRYKAW
                     GETRYTFGSEKTKFQYTGQYSYVSDFGLHYYNARWYDSSLGRFAQADSIIPIYTQGVQ
                     AWDRYAYTNNNPVLYVDPTGHSLCQILGTFLGFSLSCPSEGANDVGALINVGEKDEGG
                     ADINVPVPPDATPGIIDVPEGQMGSGALINVPGQQDDEPEGYDPAPNDGLGVIAATED
                     DESPEDILMPDGNPIGTSEDDERIRTIQTEEEMEEIFDRLTVNGTSVTKPTYEGDGYN
                     LPDGGFVGWRDSRDHGPTIDINIPGIPIDKLHLPI"
     gene            3451..3804
                     /locus_tag="VFQ23_23090"
     CDS             3451..3804
                     /locus_tag="VFQ23_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909552.1"
                     /translation="MKLQNIEKEILGLCVDDDTGFWLIIKRIAKDSYSIVSVPESVRK
                     KTMEVIRHLLQNQLIVAGNPNGPKFRACSLSTPDLIAYIVQEWDKLGSTPNIGDICWF
                     RATPAGEKLARELEV"
BASE COUNT         1046 a          976 c          905 g          907 t
ORIGIN      
        1 aggcgcgtcc tacgcctgtc tcgagcatgc caccacacca gacaggaaca ttccgtccta
       61 cacaaaaatc atgaattgca attgcctgac tcagaccacc cacgcgtgca ggcttgatgt
      121 tgatgatttt gcaggcttcc atctccaatg cataacgcgc atggcgcggt gacacgacgc
      181 tttcatcaag acagatcggt gttttgaatt ctgcctgcaa tctgcgatga tcccagatgt
      241 catcttcaaa caagggctgt tcgatcaaca gcaaattcaa atcatcaagt ggcttgagcg
      301 cgctggcaga ctccaatgtg taagcggagt tcgcgtccac ttgcaggcgt aaatcgggat
      361 atgcatttcg gacagcggaa gtctcatcga cttcgcgccc aggcttgatc ttgatcttaa
      421 cgcggcgata gcctttttcc agataagacg cgacagtact tactaaagcc gatgccgatt
      481 cctgaatgcc aatcgaaact cccacatcca ctttatcacg cgtgcccccg aacatttctt
      541 tcagcgattt gccgtcgcgt ttgccgagca aatcccacaa tgccatctcg accgcagcct
      601 tggcaaggtg atgtccgcga atcccttcca cgtgcgattg aaattcctct gcatcattta
      661 tatcttttcc caggataagc ggggcaatga agtctttgag aatatgccac gctgtgccag
      721 tcgtttcgta gctatatcct gggtcgcggg aagcgacaca ttcgccataa cccactaaac
      781 cttctgattg cgctacgatg aggatacact cacgaaccgt ctcgcgccca aatgaggttt
      841 cgaaaggggc gaccagcggc atggagatgt ggtggagggt aagggaggaa agtttcatgt
      901 ggcaagtgta taatattttt tgttacagtt caatggtaca gcttaataca aaagaatgga
      961 attgtataga gagttgtacc ggccatgaag cagctaatca atatgtggaa ttacatcaaa
     1021 ctattttggt gcgagattaa ggtatggcgc ggccttgccc gcccaattgt gctagtcaag
     1081 tttctctcaa gtcgagatca ggaaatacgc tgggatgcag gcgctgctcg acgatgccaa
     1141 cggactttac gtcacagacg atacgccgaa tgccgaacct cgctaccggg cgcgcttcta
     1201 tctcgacccg aactcgctca ccatggcaaa caacgatgcc tttttcctgt tcagaggctt
     1261 ccagggcacc tcgacagata tcctgcgagt cgaattgcgc tactcctcca gcgcgtacca
     1321 gatcagagct gcactgatgg acgatgcttc cacctgggtc aataccaact ggttcaacct
     1381 cacggatgca gcgcattata tggaggtgga ttggaaggca gcaacctccg ctggcgcaaa
     1441 caatggctat ctcaccctgt ggatcgacgg cacacaatca gccgatttga caagcgtgga
     1501 caacgacacc tggagtatcg accgtgctcg cctcggcgcg ctcaccggca tcgacgctgg
     1561 caccagcggt accttctacc tggacgcctt cgaatcccgt cgcagcagtt atattgggcc
     1621 gatggcattt ggaggtggtc acttcaccag ctatcactac gtgcctctcc agcagggcaa
     1681 ctcctacacc ggccagcccg atgccacgga tggcatcgat acctacctct tgaacacctc
     1741 gcctaccacc aataatggaa cggctgtcac gatgtggatc ggtgaaagca acaacgccac
     1801 cgacaaagtt gcgcgcagcc tgatcaaatt cgatctctcc tctattccat cgaatgcgac
     1861 gatcacctct gccactttat ctctatggac cgatgcggac ttttctgata atgaccgtac
     1921 catccgcgtc taccggttga aagtgccgtt cagtgaaact caagccacct ggaacgaggc
     1981 atctacaggt gtgaattggc aatccccggg tgcctcaggc acaaacgacc gcgaaagcac
     2041 agatattggc tgggtgttaa tccctgaaaa tcaggcactg gatgtggaga agcagatccc
     2101 actctccacc gcgcagatcc aggaaatgat caatggcaca ttcaccaata acggattcat
     2161 tattattgca gatacggaac ttaacgaccg cttcagctat aagacctcag atgccagcgc
     2221 gggaaccaaa cgtcccaaac tggtgatcga atatactacc tcttcctcga ccccaaccaa
     2281 cacgcccact cctggaccca gcccaacagc gacacgcaca cagaccccag gtgcgagccc
     2341 gacacccacc ccgactcaac cacctcaggt agctttcaac agcgcgacct tcacatacga
     2401 tggcgatgga aagcgcgtga agagcacctt caatggcgca acaaccacct actttgtagg
     2461 taatcactac gaagtcacag gttcaacggt catcaagtat tactatgctg gcgcgcaacg
     2521 aattgccatg cgaaccaatg gtacattgaa tttcttgctt ggagatcatt taggctcaac
     2581 cagcctgatt actgactcca caggcgtaaa aaccaacgag caacgataca aagcttgggg
     2641 tgaaacgcgc tatacctttg gaagtgagaa gacgaagttt caatatacgg gacaatattc
     2701 gtatgttagc gactttggat tgcattacta caacgcgcgt tggtatgata gctcgttagg
     2761 tcgttttgcg caggcggata gtattattcc tatttatact caaggtgtgc aggcttggga
     2821 taggtacgcg tacacaaata ataatcctgt tctatatgtt gatccgacgg gacatagtct
     2881 gtgccaaatt cttggaacct tccttggctt ttccttatca tgtccgagtg agggagcaaa
     2941 tgatgtagga gccttgataa atgtcggcga aaaggatgaa ggcggagctg atataaatgt
     3001 tccagttcct cctgacgcta cccctggcat aattgatgtt cctgagggac aaatgggttc
     3061 tggcgcacta atcaatgtgc cgggtcaaca agatgatgag cctgaaggtt atgatccagc
     3121 gcctaatgac ggtctcggag taatcgcggc aactgaagac gatgaaagtc cagaagatat
     3181 tctgatgcca gatggtaatc cgattggcac atctgaagat gatgaaagaa taagaacaat
     3241 acagactgaa gaagaaatgg aagaaatatt cgatagactt actgtgaatg gcacatctgt
     3301 cactaaacct acatatgaag gcgatggata taatttgccc gatggaggat ttgtaggatg
     3361 gcgagattct agggatcatg gaccaaccat tgatatcaac attcctggaa tacctatcga
     3421 taaactccat ttgccaatat aggtgttctt atgaagcttc aaaatataga aaaagaaatt
     3481 ctcggtttat gtgtagatga tgacactgga ttttggttga tcatcaaaag aatcgcgaag
     3541 gattcttatt ccattgtttc agtaccagaa tcggtacgca aaaaaactat ggaagtaatt
     3601 cgtcatttat tacaaaatca gcttattgtc gcgggaaatc caaatggtcc caaatttcgg
     3661 gcttgttctc tgtcaacccc tgatcttatc gcttatattg tacaggaatg ggataagctt
     3721 ggaagtacac ccaacatcgg cgatatatgc tggtttcgtg ccacgcccgc gggagaaaaa
     3781 ttagcccgtg aattagaggt atgacaaaaa tgaacggaca aacggtcaca aatg
//
LOCUS       DASTJP010000787         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_916019, whole genome shotgun sequence.
ACCESSION   DASTJP010000787 DASTJP010000000
VERSION     DASTJP010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916019"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2467)
                     /locus_tag="VFQ23_23095"
     CDS             complement(<1..2467)
                     /locus_tag="VFQ23_23095"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9909553.1"
                     /translation="MSERKARLHIRLMGTPEVSAEGISFALNQLKSRALLFYLAATGQ
                     SHTRDHLATLLWGETGQSKAYHSLRSSLYHLRKALQAVQADEVLISEGDLLRLDTASF
                     QCDVIEFNRLLTLGDESSLSTVVSLRRGSLLQGFSIPDAPLFDDWAQMENTRLNHACF
                     EALNRLAGWADERGAWTDAVGYVKQMIQIDPLSESAQQRLMNVYLKQGEVGLVLRQYR
                     QFENQLNQELGIAPSLETKALYEDVLRQQRNVALSVSAHHSTRPATILPFVGRDDLIH
                     ELSNISEDVEAAQGATVLIQGEGGIGKSRLIDEFASHLFSGSQPWIVLQGACSPFDDL
                     LSHGPFLEALQTGGADDLNDILAESSASVPDARGRFFWRVLQTIRSLSQSGPLLLFID
                     DLQWANGSTLNLFGFLSMRLHHLPVMLVGTVQHADAIPALQRLITLGRRRHELHLFSL
                     TPLPQEAIIELLHASDVNTSAVETLAEWLNAKSGGNPFLLSEILAQLRAEKVLKIAGD
                     GWLLDMAQWLRWRSTFSLPETTHDLVGWRLANISLDARNLLDVLAVASQPIPESVLRS
                     LPNLWNDSFPALVDDLSARGLVMELRSDRALTLPHHLLRETLLHRLSNLRRRTIHRQL
                     AEAMESQVVSDADKWLRQVALHAVAGEDVGRARRFGMSLLPHLPHEYTGAETVDFVHH
                     LHDLLAPAASPDEMVLITRALGTLHQSLGHLEVASQWHQQTLEWAQKTNNTAAQAEAY
                     FEMSELALMSIDYRTAMQAAERGLQVIHTSPGDAPLVSIGRGYRLLGASLAMEGRDLA
                     AAEDHLQKAVAVHRQIENQGDLCA"
BASE COUNT          653 a          713 c          665 g          574 t
ORIGIN      
        1 ccgcgcacag gtcgccctgg ttctcaattt ggcgatgtac agccaccgcc ttctgcaaat
       61 ggtcttctgc agcagcaagg tcgcgtccct ccatcgccag ggaggcgccg agcagacgat
      121 atccccttcc gatggacacg agcggagcat ctccagggga tgtgtggatg acttgaagac
      181 ccctttcagc cgcctgcatc gcagtccgat agtcaatgct cataagggca agttcgctca
      241 tttcaaaata cgcttcggct tgcgcggcgg tgttgttcgt cttttgcgcc cattccagag
      301 tctgctggtg ccattggctc gctacttcaa gatgaccaag cgattgatgc aatgttccca
      361 acgcacgcgt gatcaaaacc atttcatcgg gggatgcagc aggagcaagc aagtcgtgta
      421 agtggtgcac gaagtcaacg gtctctgcgc ccgtgtattc gtgcggcagg tgaggcaaca
      481 gactcatccc gaatcgccgt gcacgaccga catcttcccc agcaacggca tgcaaggcaa
      541 cttgacgcaa ccatttatct gcatcggata cgacttgtga ttccatcgcc tccgccagtt
      601 gacgatgaat cgttcggcgg cgaagattgc tcaaacgatg cagcaacgtc tcgcgcaaaa
      661 ggtgatgcgg taatgtcagc gccctatcac tgcgcagctc cataacgagc ccgcgtgcgg
      721 atagatcgtc aacaagtgca gggaacgaat cattccagag gttaggaagg cttcggagaa
      781 ccgattcagg tattggctgg gaagcgaccg ccagaacatc cagcagattg cgcgcatcga
      841 gcgagatgtt tgcgaggcgc cagccaacca ggtcgtgggt tgtctcaggc aatgaaaaag
      901 tagagcgcca ccgtagccat tgtgccatat ctaacagcca gccatcgccg gcgattttca
      961 gaactttttc cgcacgcaat tgcgcgagaa tttcggaaag caagaacgga ttgccccctg
     1021 actttgcatt cagccattcc gcgagagttt ccacagcgct tgtgttgaca tcggatgcgt
     1081 ggagaagctc tatgatagct tcttggggta atggagtcag cgaaaacaaa tggagttcat
     1141 ggcggcggcg tccgagcgta attaatctct gcaatgcagg gatggcatct gcatgttgca
     1201 ctgtgccaac cagcatcaca ggcagatgat gaagccgcat ggaaagaaag ccgaacaaat
     1261 tgagcgtcga accattcgcc cattgcaggt cgtcaatgaa aagcagcagt ggcccgcttt
     1321 gagagaggga gcgaatcgtt tgcagcactc gccagaagaa acggccgcgc gcatccggaa
     1381 cgctggcact cgattcagcg aggatgtcat tcaagtcatc tgcacctcct gtctgcagag
     1441 cttcgaggaa gggtccatgc gaaagcaaat catcaaacgg cgaacaggcg ccttgtaaaa
     1501 cgatccaggg ttgtgaacca gaaaagagat gggatgcaaa ttcatctatg agccgactct
     1561 tgccgattcc gccctcgcct tgaatcaaaa ccgtcgcgcc ctgcgctgcc tccacatcct
     1621 cactaatatt tgataactcg tgaattaaat catcgcgccc gacaaagggg agaatagtag
     1681 ccggacgtgt tgaatgatgc gccgagacac ttagggcaac atttctttgc tgccgaagga
     1741 catcttcgta caacgctttg gtttccaggg atggcgcgat cccaagctcc tggttcagct
     1801 gattctcgaa ctggcgatac tgacgcaata ccagcccgac ctccccctgc ttgagatata
     1861 cgttcatcaa tcgttgttgc gccgactcgg acagcgggtc aatttggatc atttgtttga
     1921 cgtaaccaac ggcatctgtc catgctccac gctcgtccgc ccaacctgcc aaacgattca
     1981 atgcttcaaa acaagcgtgg ttgagacgcg tattttccat ctgcgcccaa tcatcgaaca
     2041 agggtgcgtc gggaatggaa aatccctgca acagggaacc tcttcgcaat gagacaaccg
     2101 tagagagcga actttcatca cccagagtca gtaaccgatt gaactcaatt acatcgcact
     2161 ggaaagaggc tgtgtcaaga cgcagcaagt cgccttcact gattaagact tcatccgctt
     2221 gaacggcttg caaggcttta cgcaaatgat acagactgga acgcagcgaa tgataggctt
     2281 tactctgacc cgtttcgccc cacagcagag tcgccaaatg gtcgcgggta tgggattgtc
     2341 cagtcgccgc aaggtaaaac aataaggcgc gtgatttaag ttgattcaag gcaaatgata
     2401 tcccctctgc ggaaacttct ggagttccca tcaatcgaat gtgtagacgc gcctttcttt
     2461 cagacattca tctacctgta aacttcagta agttgaattt taaccttatt tattttaccc
     2521 gactcacttg aagcaaacga attctcttct acccgagcag caactccggc cgactgcgac
     2581 actccacggc actccctggc accgt
//
LOCUS       DASTJP010000788        13682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_418297, whole genome shotgun sequence.
ACCESSION   DASTJP010000788 DASTJP010000000
VERSION     DASTJP010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13682
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418297"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VFQ23_23100"
     CDS             <1..1532
                     /locus_tag="VFQ23_23100"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9909554.1"
                     /translation="AFRLSDGADPFMPIASFLNSIFGASQTGVHVAWWVSLAWVLYIL
                     PYITRSKHTHFTVGPINLGLAKQNPRGQLDPAIPLKVVSGNHYEPGAKFLHDLSWKRV
                     LDSYACVQCNRCQDVCPADFYGRPLSPSALEVNKRYLLKDATFGSHPDRLLIPLTETV
                     ISPEAVWSCTTCYACVRVCPVGNEPMADIIDIRRRMIMEGAELDSGVQNALQSLATNG
                     NWMSKGKRLRGRWAKEMEFPIKNAIEEPVEHLWFVGDTASLDERVIPNTKMVARVFQC
                     AGLDFGIMYQNESNAGNDVRRVGEEGLFEQLVEQNLQAFGKAQFKRILTTDPHSFNTL
                     KNEYPQFGGNFDVKHYTAVLVRLFEEGKLTIKNKLPHYKVTFHDPCYLGRYNGGFSAP
                     RKLLALLGVEFSEMPRNCENSFCCGAGGGQIWMGKVASGERPAENRIKEALTTFEKNP
                     SNKKQLFIVTCPKDMIMYSDAVKTTGNEAKIEVRDLIQLVAEAVGVEEPAMTEAVASV
                     A"
     gene            1537..1821
                     /locus_tag="VFQ23_23105"
     CDS             1537..1821
                     /locus_tag="VFQ23_23105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909555.1"
                     /translation="MFKCPYCEFRGKRSEVHNHLAESHPDTLVSRVDEFTGHTFYVVT
                     CPVCGASYEQVTKKARRDPSFVSEYEHEIHLVVFDLLLYHLQGEHGEGTS"
     gene            1838..2275
                     /gene="gcvH"
                     /locus_tag="VFQ23_23110"
     CDS             1838..2275
                     /gene="gcvH"
                     /locus_tag="VFQ23_23110"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9909556.1"
                     /translation="MAQVRGCSIPEDRFYWVEKHTWARPEDDGTVTIGITDVAQNLAK
                     GIINATPKDVGRTVQKGKSAGTLESGKWVGPVTSPITGEIIAVNEAMKAKPSLINSDP
                     YGEGWFVKVKPADWAGESALLVTGEEGVAAYQKFMEEQNISFE"
     gene            2305..2751
                     /locus_tag="VFQ23_23115"
     CDS             2305..2751
                     /locus_tag="VFQ23_23115"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909557.1"
                     /translation="MIYYACDIPEDLTYDIERDVWIRFEGNLAILGMTDVAQSRCGKI
                     AAISFRDVGKKVVQGKALATIESAKWVGPFPAPFSCEIVETNEEGFARDILITNKEPY
                     TTGWLVKVRPTNLEAERGHLVTGEEAAEKYKVRIQELKINCLRCID"
     gene            2870..3955
                     /locus_tag="VFQ23_23120"
     CDS             2870..3955
                     /locus_tag="VFQ23_23120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HET9909558.1"
                     /translation="MSRKIRLLYMEGVSPVRSQTIYHAVGYAMKEDTPDTIILVSPNA
                     PYVCIGYHQDIEKEVDLEYCRAHSLPVYRREVGGGAVYLDNGQLFTQWIFHKENLPAS
                     LEERFKLYIDPLVATYQALGINATHRPVNDVHVNGKKIGGTGAAQIGSAEVVVGSLMY
                     TFDKKTMSQVLKVPSEKMRDKIFESLEAYMTTMTEQLGTTPDRQHVKDLYMQKVSEVL
                     GVEVYEGEWTAEEEAMATDLDARFVSEEWLYQKGRLRQPGVKIHEDVHIVEAAFKAQG
                     GLIRVIARLHEGRIDDVSMSGDFTLMPSFALGALEQSIRGMRATPETLEARLREAYNR
                     LSIQSPGVTPADFTTAIMNAVTPPQPA"
     gene            4003..5691
                     /locus_tag="VFQ23_23125"
     CDS             4003..5691
                     /locus_tag="VFQ23_23125"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9909559.1"
                     /translation="MPNYGFLIDQSKCIGCHACSTACKSENQVPLGVYRTWVKYTETG
                     AYPDVRRRFQVTRCNHCANPPCVRICPVSAMYQRSDGIVEFDPSICIGCKSCMQACPY
                     DSIYLDPETNTAAKCHFCAHRLDVGLEPACVVVCPEHAILAGDLNDPASEISRQLASA
                     QATVRKPEQGTGPKLFYINGNDWSLHPTASPVSNSYVWADKITEQNTNPYEHQSFQLP
                     VIQSPRGNGHSSIRQKVKKSKSGTPIRTPREQGRPLNGPVQIGGRVAEHMVQTAYNAQ
                     HKVQWHWELPSYLVTKNIAGGLFMLLSLGSGLGAFPFDSLTFLAAGFTALIFMLLTVI
                     LLIKDLSQPKRFLNVLLRPQWKSWLARGAYIMVTFTAVAGLWWLLEGAAYLNWLLADF
                     AVGIRPFAAWITFPLALGVVIYSAFLLGQAEGRDMWQSPLLPFELLAQSIVVSSGVFL
                     GLSLVVALPADLQQLLSVVFPVSLAINLLLMLANKFAMPFSSEIAMLASRDITHGQYR
                     NHFWWGGIAIGHVLPFALFLTVPVTVPIAALTAIIGLFFYEYAFVMAPQRIPNS"
     gene            5732..8644
                     /locus_tag="VFQ23_23130"
     CDS             5732..8644
                     /locus_tag="VFQ23_23130"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9909560.1"
                     /translation="MTKTKNIPQTSAGTSVPQFSASDRKPVKLTEVVHPDGRISQYPP
                     PEVWDDWVEWDGKAWPQRVARRYTLVPTVCFNCESACGLLAYVDKETYEIRKFEGNPV
                     HPGSRGRNCAKGPATHNQVYDPERILYPLKRAGKRGEGKWERITWDQALNEIAEKMRE
                     SRARRRDGIVYHVGRPGEDGYTNRVIQAWGVDGHNSHTNICSSSSRAGYNFWTGQDRP
                     SPDYANARTILLISSHLETGHYFNPHAQRIIEAKTNGAKLIVFDPRLSNTASMSDAWL
                     PTWPGSENTILLAIANYLIQNDRYNKDFVRKWVNWKEFLQYVQSSSSNEWNAIRHLIS
                     DPLSFDVFDRVLKTLYSEYTFERAAQESEVPMERIEQAAKYVADCDGRLATHVWRSAS
                     VGNLGGWQVSRCLFFLNVLTGSIGNYGGTSMINWNKFVPKPFKNPPPINAWNELHYPK
                     EWPLSHYEMSFLLPHMLEEGRGDLDVYFTRVYNPMWVNPDGFMWLKALQDESKIKCYV
                     ALTPTWNETAWFADYVLPMGHGPERHDLMSQETHSGQWIAFRQPVRRVALERAGTKVD
                     FTYQANPGEVWEENEFWIQLSAHFDPDGSLGIRQYFESPYRPGQIITVDEYYQWIFEN
                     SVPGLPEAAANEGCTPLDYMRKYGVFEVKKENYIPFEKAPQAAGETEFIFDQHDRVLD
                     ASGGVVGLMVDGQPKAGFDTPSRRLEFFSPTLSAWGWDEKQYVIPWSVKSHVHPDNID
                     REKGEMILLPNFRLPTLIHTRSANAKWLYEISHKNPIWINPADAKRIGVETGDLARVD
                     TEIGYFVDSIWVTEGIKPGVIAISHHLGRWRLQEDSGVNRGASSLVKLTDDSKGGFLL
                     RVIHGAQPYESSDPDTSRIWWSEVGVSQNLTHGVHPDPVSGVHCWLQKAKSVRKAHDD
                     EKAGDVFVDTNRSMEIYRQWLATTRPADQVSPDGTRRPYWLTRPLKPTKEAYKLPT"
     gene            8663..9733
                     /locus_tag="VFQ23_23135"
     CDS             8663..9733
                     /locus_tag="VFQ23_23135"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HET9909561.1"
                     /translation="MTEIFTPSPKADETKTQDAEEPVWTYRGYRLKTSEFVTAMVHLF
                     RAEIQRANVWRQRLDTTTNWAVVSTGATLSIAFSQPDIHHGIIILNTLLVMWFLLIEA
                     RRYRYYELWSYRVRLMETDFYAAMLVPPFHPSPEWAESLAENLLSPKFPISIWEAFGR
                     RLRRNYFWIFLILYASWAAKIWLFPQPAVNLNEIIQRSAVGLISGEIMILLGFGLYAF
                     LLIVALATISMTRATGEVLPRFGEDTITSMFSSNGKPREARLFFAPRHRRRQLVALII
                     TGKAEAVSKRIMADLSRGVTALAGKGMYTGQDRSVLICALTVTEVHNLKHAVAKEDPS
                     AFVVVSAAQEILGRGFNPLAET"
     gene            9733..10359
                     /locus_tag="VFQ23_23140"
     CDS             9733..10359
                     /locus_tag="VFQ23_23140"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HET9909562.1"
                     /translation="MQPLEMILRESAKRHKHLCPRQVLGARMGILAGEILKLDLPRAD
                     KKLLVIAETDGCTVDGLIAATGCHVGGRTLRILDFGKVAATFVDSYTEGAIRIAPNDK
                     ARTLSFAYAANARNRWEAMLLGYQVMPVEELFCVQYVHLNLSLEEIVSKLSKKAQCEI
                     CHEEIINGREVTHDGSVLCRSCAGEDYYEVLDVSRSLVSSAPHRLCCH"
     gene            complement(10642..11619)
                     /locus_tag="VFQ23_23145"
     CDS             complement(10642..11619)
                     /locus_tag="VFQ23_23145"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HET9909563.1"
                     /translation="MLKDDEFDSQVIALLDELKPAPARNPQIAARARARFLAEAISVR
                     DESRLSIWTIFQQKEKFAMKLILSTFVIVGLLFGGSATVSAAQDDLPNELLYQIKLVS
                     EDVTLWWVSDPSAQIEILMQQAQTRTEEMAALVSRGDTPPAELALRAQERIQRALQLA
                     ASLDDASQTATLQQIRTRLQTQEQLMIQLQDGTCSACEPILQQTRDMLRARLRQIENG
                     LVEPEPFQNQNQNQLRTTQTPQPPNSIFTPQGTCCTPALNGTGQQNGNGSKNPSTGTP
                     VPQNNTTNQGGSGPQNGNNSGNGNGAQSGEGSENGGGSGPGSGGQGGKP"
     gene            complement(11606..12151)
                     /locus_tag="VFQ23_23150"
     CDS             complement(11606..12151)
                     /locus_tag="VFQ23_23150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9909564.1"
                     /translation="MISEQDLLLRARQLDPCALEEIYDLYSPRLYRYAVRLLGDACTA
                     EDCVAETFGRFLQAIRTRKGPSDFLQAYLYRTIHNLVVDHYRRQSPLLLLNDDSSGFG
                     NIEETADQNIRKLRVRAAIHELTSDQQQVIALKFLEGWENEEIAHILNKPVGAVKSLQ
                     HRALASLQKILLNDEVEHVER"
     gene            12420..13274
                     /locus_tag="VFQ23_23155"
     CDS             12420..13274
                     /locus_tag="VFQ23_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET9909565.1"
                     /translation="MVTLFGKRIRIFRPRTIWIRKIVQWFFFLLIALIAVNHTLAENG
                     RGIPILSSASLHAVCPFGGVETLYTLAAGGILVKKIHDSSLVLAGIVLVLSILFGPVF
                     CGWVCPLGTVQEWVGKIGKKLFRRRYNHFVPARLDNVLRYARYGILLWVLYITATSGT
                     LIFEAYDPYFALFNFWSTEVAWSAIAILGITLVLSVFVERPWCKYACPYGAVLGITNL
                     FRVFSIKRSETTCKADGACSIMCPMNIPVDTKKVVRDHQCISCLECTSEAICPVANTV
                     LFTGGGVK"
     gene            13271..>13682
                     /locus_tag="VFQ23_23160"
     CDS             13271..>13682
                     /locus_tag="VFQ23_23160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909566.1"
                     /translation="MTLTSKSLAVIVLAMVFGGIAFSSVMGWWATESTKQPVTFADGE
                     FAGQADPADIRGSYTFSDIANSFDVTPEVLAQAFGITESDPAAFAVKELEAIYLDSGF
                     EIGTASVRLFVAFYSGLPFDTTEQEIYMPQSATDI"
BASE COUNT         3260 a         3415 c         3608 g         3399 t
ORIGIN      
        1 aggcctttcg tctcagcgac ggcgcggatc cattcatgcc gattgcaagt ttcctcaaca
       61 gtattttcgg agcgtcgcaa acgggtgtcc atgttgcgtg gtgggtaagc cttgcatggg
      121 tactgtacat tctgccttat attactcgca gcaagcacac gcatttcact gtcggtccca
      181 tcaatctcgg acttgccaaa caaaatccac gcgggcagct cgaccctgca attccgctca
      241 aagtcgtgag tggcaatcac tacgaacccg gagcaaaatt tttacatgac ctctcatgga
      301 aacgtgtttt ggattcttat gcctgcgtgc aatgcaatcg ctgtcaggat gtatgtcctg
      361 cggatttcta tggacgtccg ctctcgcctt cggccctgga ggtaaataag cgttatctcc
      421 ttaaggatgc gaccttcggt tctcaccctg accgactact gatcccgctt actgagacag
      481 tgatctcccc ggaggcggtc tggtcttgca caacatgtta tgcatgcgtg cgcgtgtgtc
      541 ctgttgggaa tgagccaatg gcagacatca tcgatatccg tcgccgcatg attatggaag
      601 gggcagagct ggatagcggt gtgcagaacg cattacagtc ccttgctaca aatggcaact
      661 ggatgagcaa aggaaagcgt ctgcgtggac gctgggcaaa ggagatggag tttccaatca
      721 agaacgcgat cgaagagcca gtggagcatc tctggtttgt gggggatacc gcctcgctcg
      781 atgagcgcgt gattccaaac acaaagatgg tcgcgcgggt cttccaatgc gcggggttgg
      841 attttggaat tatgtatcaa aacgaatcta atgcaggtaa tgatgtccgc cgcgttggcg
      901 aggaaggtct cttcgagcag ttggttgagc agaatcttca agcttttggt aaagcgcagt
      961 tcaaacgaat tctcactact gacccgcact cgttcaatac gttgaaaaat gagtatccgc
     1021 aattcggtgg gaactttgac gtgaagcatt acacagcagt tttggttcgg ttgttcgagg
     1081 aaggaaaatt aaccatcaag aacaaactgc cgcattacaa ggttaccttc cacgatccat
     1141 gctaccttgg tcgctacaac ggaggatttt ccgcgccgcg caaattgctt gccttgctgg
     1201 gcgttgaatt cagcgaaatg ccgcgcaact gcgagaactc attttgctgt ggcgccgggg
     1261 gtggacagat ctggatggga aaagtcgctt ccggcgaacg ccctgcagag aatcgaatca
     1321 aagaagcgtt gactacattt gagaaaaatc ccagcaataa aaaacaattg tttattgtca
     1381 cctgccccaa agacatgatc atgtattccg atgcggtcaa gacaaccggt aacgaagcga
     1441 aaatcgaagt ccgtgatctg attcagctgg tggcggaagc cgttggcgtg gaagaacctg
     1501 caatgacgga agctgttgca agcgttgcgt gagccaatgt tcaagtgccc gtattgcgaa
     1561 ttccgcggta aacgttccga agtccacaat catctggcgg agagtcatcc ggacacattg
     1621 gtctcgcgcg tagacgagtt tacaggtcat acgttttatg tcgtaacgtg tcctgtctgt
     1681 ggcgcgagtt atgagcaagt gacgaaaaag gcgcgccgcg atccgagttt tgtctcggag
     1741 tatgaacacg agattcattt ggtcgtcttt gatttactcc tctatcacct gcagggtgaa
     1801 catggagaag gaacatcata aataatcgga gggcagcatg gctcaagtac gtggatgcag
     1861 tattcctgaa gaccgcttct actgggtgga aaaacacacg tgggcgcggc ccgaggacga
     1921 tggaacggta accattggga ttaccgacgt ggcacagaat ttggcaaagg gcattatcaa
     1981 tgccacgcca aaagatgtgg ggcgtaccgt gcagaaaggc aagagcgccg ggacgttgga
     2041 aagcgggaag tgggtgggac ccgtgacatc gcccataact ggtgagatta tcgccgtcaa
     2101 cgaggcgatg aaagcaaaac cttcattgat caacagtgac ccgtatggtg aaggctggtt
     2161 tgtcaaagtg aagccagcag actgggccgg cgaaagcgca ttgctcgtga cgggggagga
     2221 gggcgtggcg gcgtatcaga agtttatgga agagcaaaat atctcgtttg agtaaaacgc
     2281 ttgaacgttc aaatgtaatc gccgatgatt tattacgcct gtgatattcc tgaggatctg
     2341 acctacgata tcgaacgcga tgtgtggatt cgattcgaag gcaacctcgc cattctcggc
     2401 atgactgatg tcgcgcagtc acgttgcgga aaaatcgcgg cgatcagttt tcgcgatgtt
     2461 gggaaaaaag ttgtgcaggg caaggccttg gcgaccattg aaagcgccaa atgggtagga
     2521 ccattccctg cgccgttttc atgtgaaatc gttgaaacga atgaagaagg ttttgctcgc
     2581 gatattttga ttacgaacaa agagccttac accactggct ggcttgttaa agtgagaccg
     2641 acgaatctcg aagcagagcg cggtcatttg gtcactggcg aggaagcagc tgagaagtat
     2701 aaggtgagaa tacaggaatt gaagataaat tgtttgagat gcattgatta acctgtcatt
     2761 gcgagcgagg gtttttcagg gagcaaagca atctattaat tgctttgaag attgcttcgt
     2821 cggggagaac gccctcctcg caatgacata aaactaaaag aggtgactca tgtctcgaaa
     2881 aatccgcttg ttgtatatgg aaggcgtttc cccggttcgc tctcagacca tctatcacgc
     2941 ggtcggctat gcaatgaaag aagacacccc tgataccatc attcttgtct cgccaaacgc
     3001 gccgtatgta tgtattggct atcaccagga catcgaaaaa gaagttgatt tggaatattg
     3061 cagggcacac agtctgcctg tctatcggcg agaagttggt ggtggtgcag tatatctgga
     3121 taacggtcag ctatttacac agtggatttt ccataaggaa aacctccctg cctcgttgga
     3181 agaacgtttc aaactatata tcgatccgct tgttgcgacc tatcaggcat tgggtatcaa
     3241 tgccacccat cgtcccgtaa atgatgttca tgtcaatggg aagaaaatcg gtgggacagg
     3301 cgcggcacag attggctccg cagaagtagt ggttggcagc ttgatgtaca catttgacaa
     3361 aaagaccatg tcacaggtac taaaagtccc ttcggagaaa atgcgcgaca agatttttga
     3421 atcgctcgaa gcctacatga caaccatgac cgaacagctt ggcacaaccc ccgaccgcca
     3481 gcacgttaaa gatctttaca tgcagaaggt ctctgaagtg ctgggggtgg aggtctacga
     3541 aggcgagtgg acggctgaag aggaagcgat ggctaccgac ctcgacgcac ggttcgtttc
     3601 ggaagaatgg ctctatcaga aggggcgctt gcgccagccc ggagtcaaaa tccatgagga
     3661 tgtacacatt gtcgaagcag cgttcaaggc acagggtggt ctaatccggg tgatcgctcg
     3721 cctgcatgaa gggcgcattg atgatgtttc catgtcgggc gactttaccc tcatgccgtc
     3781 gtttgcattg ggcgcactgg aacagtccat tcgtggaatg cgcgctacac ccgaaacact
     3841 tgaggcgcgt ctgcgagaag cctacaaccg gttgagcatt cagtcccctg gcgtcactcc
     3901 tgcagacttc acgaccgcga tcatgaacgc ggtcacacct ccgcaacctg cataatgagt
     3961 taaacacaga ggatcaagtc tttcatcctt ataaaggtat tcatgcccaa ttacggattt
     4021 ttgattgatc aaagcaaatg tattggctgt catgcctgtt ccaccgcgtg caagtctgag
     4081 aaccaggtgc cgctgggtgt gtaccgcacg tgggtgaaat ataccgagac aggtgcatat
     4141 cccgatgtgc gccgtcgctt tcaggtgaca cgctgtaatc actgtgccaa tccgccttgt
     4201 gtgcggatct gtccggtcag cgcgatgtac cagcgcagtg atgggatcgt cgaattcgat
     4261 ccgtctatct gcatcggatg taaatcctgt atgcaagcct gtccctatga ctcgatttat
     4321 cttgatcctg aaaccaacac ggccgcgaaa tgtcacttct gtgcccatcg cctggatgta
     4381 ggactcgaac ccgcttgcgt tgttgtctgc cccgaacacg cgattctggc tggagacttg
     4441 aacgatcccg cttcagagat cagccgacag cttgccagcg cacaggcgac cgttcgtaag
     4501 cccgagcagg ggaccggacc taaactcttc tacatcaatg gcaacgattg gtccttgcat
     4561 cctacggctt cacctgtaag taattcctac gtgtgggcag ataagatcac tgagcagaat
     4621 acaaatccct atgagcatca atcgtttcaa ttgcctgtaa ttcagtcacc ccggggtaac
     4681 gggcactctt caatacggca gaaagtcaag aaatccaaat cagggacacc gatccgcacg
     4741 ccgcgcgaac agggacgacc gctcaatggt cccgtccaaa ttggcgggcg tgttgccgag
     4801 cacatggtcc agactgccta taacgcccag cataaggtcc aatggcattg ggaattgccc
     4861 tcctacctcg tcaccaaaaa catcgcgggc gggcttttca tgctccttag tctcggctcg
     4921 ggacttggcg cattcccctt tgattcgctt acgtttctcg ccgcaggctt cactgcgttg
     4981 atcttcatgc ttctcactgt tattctactg atcaaagatc tgtcgcaacc caaacgcttc
     5041 ctcaacgttt tgctgcgccc acaatggaaa tcgtggctgg cgcgcggcgc ctacattatg
     5101 gttacgttta cggctgtcgc agggttatgg tggctgctcg aaggcgcggc atatttgaac
     5161 tggcttctcg ccgacttcgc agtgggcatc cgccctttcg cggcgtggat aacattcccg
     5221 cttgcactgg gcgtagtcat ttactctgcc tttctgctcg gtcaagctga gggacgcgat
     5281 atgtggcaga gtcccttgct tccttttgaa ctactcgcac aatccattgt ggtatcgagt
     5341 ggcgtatttc ttggactgag tctggtcgtc gcactccccg cggacctgca acaactttta
     5401 tctgtcgttt ttcctgtcag tttggcgatc aacttgctcc tgatgctggc aaacaagttc
     5461 gccatgccgt tttcctccga aattgcaatg cttgcctcgc gcgatattac acacggtcaa
     5521 taccgcaatc atttctggtg gggtggtatt gcaatcggac atgttcttcc attcgcgttg
     5581 ttcttgaccg tcccggtgac cgtgcccatt gctgccctca ctgcgatcat agggttattc
     5641 ttctatgaat acgctttcgt catggctccg cagagaatac caaattcatg attcgctaac
     5701 cgaatagcga atacttacta ttgagtaacg aatgacaaaa acaaaaaaca tccctcaaac
     5761 ctctgcaggc acgtccgtcc ctcaattttc cgcgtcggat cgtaaacctg tcaaattgac
     5821 cgaagttgta caccctgatg ggcgcatcag ccagtaccca cctcctgaag tatgggatga
     5881 ctgggtggaa tgggacggca aggcctggcc gcaacgcgtc gcacggcgat atacgcttgt
     5941 gccaaccgtt tgcttcaact gtgaatcggc ctgtggattg ctggcctatg tggataaaga
     6001 gacgtatgag attcgcaagt tcgagggcaa ccctgtccat cccggaagcc gtggacgaaa
     6061 ctgcgccaag ggtcctgcca ctcacaatca ggtctatgac cccgagcgga ttctgtatcc
     6121 actcaaacgg gctggaaaac gcggggaagg aaaatgggaa cgcatcacct gggatcaggc
     6181 cctgaatgaa atcgcagaga aaatgcgtga gagccgcgcc cgacgccgtg atgggatcgt
     6241 ctatcacgtg gggagaccag gcgaggatgg ctacactaat cgtgtgatcc aggcctgggg
     6301 cgtggacggt cacaacagcc acaccaatat ttgctcgagc agttcacgcg cgggctacaa
     6361 tttctggaca gggcaggatc gacccagtcc tgattacgct aacgcgcgca cgatactctt
     6421 gatctcaagc catctcgaaa cgggacatta cttcaatcct cacgctcagc gcattattga
     6481 agcgaagacg aatggtgcga agctaattgt cttcgatccg cgcctctcga acacggccag
     6541 catgagcgac gcctggttgc cgacctggcc cggcagtgag aacacaatcc tgttggcaat
     6601 tgccaactat ctcattcaaa acgatcgcta taacaaagac ttcgtccgca agtgggtaaa
     6661 ttggaaagag ttccttcaat acgttcaatc atcatcatcg aacgaatgga acgcaatccg
     6721 ccacttaatc tctgaccccc tttcttttga tgtctttgat agggtgctaa aaactcttta
     6781 ctctgaatat accttcgagc gcgccgctca ggaatcagag gttccgatgg aacggatcga
     6841 acaagccgcg aaatatgttg ctgattgcga tggcaggctt gcgactcacg tttggcggag
     6901 tgccagcgtt ggcaatctcg ggggatggca agtctcgcgc tgtttgttct tcctcaatgt
     6961 gctcacgggg agcattggca attatggcgg cacatccatg atcaactgga ataaattcgt
     7021 gccaaagccg ttcaaaaatc cgccgccgat caacgcctgg aatgaattgc actatccaaa
     7081 ggaatggccg ctttctcatt acgaaatgtc cttccttctt ccacacatgc ttgaagaggg
     7141 gcgcggcgat cttgacgttt atttcacgcg cgtctacaac ccgatgtggg tcaacccgga
     7201 cggtttcatg tggctcaagg cgctccagga tgaatccaaa ataaagtgtt atgttgctct
     7261 cacacccacc tggaatgaaa cggcgtggtt cgcggattac gttttgccaa tgggtcacgg
     7321 tccagaacgt cacgatctga tgagtcagga aacgcattcc ggccagtgga ttgccttccg
     7381 ccaacccgtc cgccgcgtgg cactcgaacg cgcgggcacc aaagttgact tcacttacca
     7441 agccaaccct ggcgaagtct gggaggagaa cgaattctgg atccaacttt ctgcccattt
     7501 tgaccctgac ggctcgctcg gcattcgaca atattttgag agcccttatc gcccaggtca
     7561 aattatcacg gtggatgaat actatcaatg gatttttgag aactccgtcc cagggcttcc
     7621 ggaagctgcc gccaacgagg gatgcacccc gcttgactac atgcgcaagt atggtgtctt
     7681 tgaagttaag aaagagaatt acattccgtt tgaaaaagcg cctcaagccg cgggcgaaac
     7741 cgaatttatc tttgatcagc atgatcgtgt cctggatgcc tccggtggag tcgttggact
     7801 gatggtggat ggccagccaa aggctgggtt tgacacgcca agccgcagat tggaattttt
     7861 cagccccacg ttatccgcct gggggtggga tgagaagcag tatgtcatcc cctggtcggt
     7921 gaagagccat gtgcaccctg ataacatcga ccgtgaaaaa ggtgagatga ttttgcttcc
     7981 caacttccgc ttgcccacat tgattcacac gcgcagcgca aatgcaaagt ggttgtatga
     8041 aatcagtcac aagaacccga tctggataaa cccagctgac gcaaaacgaa ttggtgtgga
     8101 aactggcgac ctggcgcggg tggataccga gattggttac tttgtggaca gcatctgggt
     8161 cactgaagga atcaaaccgg gtgtcatcgc catcagccat catcttgggc gctggcgctt
     8221 gcaggaagac tccggcgtca atcgcggggc ttcgagtttg gtgaaactga ctgatgacag
     8281 caaaggcggg ttccttttgc gcgttattca cggtgcgcaa ccctatgaat ccagcgaccc
     8341 tgatacaagt cggatctggt ggagtgaagt aggtgtttcg caaaacctga cgcacggcgt
     8401 tcatcctgac cctgtgagtg gagtgcattg ctggctgcaa aaagcgaaga gcgttcgaaa
     8461 agcgcacgat gatgaaaaag cgggtgatgt tttcgtagat acaaatcgct caatggaaat
     8521 ttatcggcaa tggcttgcaa ccacccgccc cgcggaccag gtcagtcctg acggtacgcg
     8581 gcgaccatac tggctcacgc ggcctctcaa gccgacgaag gaagcttaca aattgcctac
     8641 atgatgttgc ctggaagaca gaatgactga aatttttaca ccctcaccaa aagcggatga
     8701 aacgaaaacg caagatgccg aggagcccgt ctggacatac cgcggctatc gcttgaagac
     8761 cagtgagttt gtcactgcga tggttcatct tttccgcgca gaaatccagc gcgcgaatgt
     8821 ctggcgtcaa cggctggata ccacgacgaa ctgggccgtc gtttcaaccg gcgcgaccct
     8881 gtcgattgca tttagtcaac ccgatattca tcatggaatc attatcctca atacattgct
     8941 cgtaatgtgg ttccttctta ttgaagcgcg gcgttatcgc tactatgagc tgtggagtta
     9001 ccgggtgcga ctgatggaaa cagattttta cgcagccatg ctcgtcccgc ccttccatcc
     9061 ttcccctgaa tgggcggaga gtcttgccga gaatttgctt tcccccaagt ttcctatctc
     9121 aatatgggag gcgttcgggc gtcgcctgcg cagaaattac ttctggatat tcttgatcct
     9181 ttacgcgtca tgggcggcga agatctggct tttccctcag ccagcagtga acttgaatga
     9241 aatcatccag cgtagtgcag tgggactcat ctccggcgag atcatgattc tgttaggatt
     9301 tggtttatat gcttttcttc tcattgtcgc gcttgctacg atcagtatga cacgtgcgac
     9361 cggtgaagtg ctgccacggt tcggcgaaga tacgatcaca tccatgttct cctcgaatgg
     9421 caaacccagg gaagcgcgcc tattctttgc cccgcgtcat cgacggcgcc aactggtagc
     9481 cctgatcatt actggaaagg cagaagcagt atcgaagcgg atcatggctg atctgagccg
     9541 cggcgtgacc gcattagcgg gcaagggcat gtacactggt caggatcgct cggttttgat
     9601 ctgcgcatta acagtgacgg aagttcacaa tttgaaacat gcagttgcaa aagaagatcc
     9661 ctctgccttt gtggtcgtct ccgcggcaca ggagattctg gggcgtggtt tcaatccact
     9721 tgcagaaaca taatgcaacc gctggaaatg atccttcgtg aaagcgcaaa acgccataaa
     9781 catttgtgtc cacgtcaggt gttgggtgcg cgtatgggaa tattggcggg ggagattcta
     9841 aaactggatt tgcctcgagc ggataaaaaa ttgcttgtca ttgcagaaac cgatggatgt
     9901 accgtggatg ggctcatcgc cgcgacaggt tgtcatgtgg gaggacgtac gcttcggatt
     9961 cttgactttg ggaaggttgc cgcaacattt gttgatagct acactgaagg agcgatccgt
    10021 attgctccca atgacaaagc ccgtactctt tcattcgctt atgccgctaa cgcgcgaaat
    10081 cgctgggaag ccatgttgtt gggctatcaa gtgatgcctg ttgaagagct gttttgtgtt
    10141 cagtacgtgc atctaaattt gtcattggaa gagatcgtga gcaaactcag caaaaaagct
    10201 caatgtgaaa tatgtcatga agaaattatt aatgggcgtg aagtgacaca tgatggttca
    10261 gtcttgtgcc gctcatgtgc aggggaagat tattatgaag ttttggacgt atccagatcc
    10321 ctagtcagct cagcgccgca ccgcctttgc tgtcattaat agctgcttga acaaaacagg
    10381 atacgtagaa agcctcagtt catcttcgct ctaaatcaaa acaaaagttc caagaaggtt
    10441 tatctggagt ggtgtatgcc gtttgacatc gctccgtaag gcatctaccg cctgttgcac
    10501 ttgtttatgc cggttgcggc cgtatttccc tggcacactc tgttccgtga caaagctcgc
    10561 ctggtaaacc ctctccggct ggactggcaa ctcgccaata aagtgcttcc tgctgaaaac
    10621 agcaggaagc actttgattt gttagggctt tccgccctga ccaccggacc caggccccga
    10681 accaccgcca ttctcgctgc cttcaccact ttgcgcgcca ttgccattcc cgctgttgtt
    10741 gccgttttgt gggccactac caccctgatt tgttgtgttg ttttgcggca cgggtgttcc
    10801 agtagacgga ttcttgcttc cattcccgtt ttgttgacct gtgccgttca gggcgggagt
    10861 gcagcaagtt ccctgtggcg taaagatgct attgggaggt tgcggagttt gtgtggtacg
    10921 caactggttc tgattttggt tttggaaagg ttccggttcg acgagaccat tttcaatttg
    10981 tctcaatcga gcccgtaaca tatcgcgtgt ctgttgcagg attggttcac acgcagagca
    11041 agtaccatcc tgaagttgga tcatcaattg ttcctgtgtt tgcaagcgcg ttcggatttg
    11101 ttgaagtgta gctgtctgtg atgcatcgtc gagacttgca gccagttgga gggcgcgttg
    11161 aattcgttcc tgcgcccgca aggccaattc ggcgggcggc gtatctcctc tcgagaccag
    11221 tgctgccatt tcttcggtgc gagtctgcgc ctgttgcatg agtatttcga tttgtgcact
    11281 gggatcagat acccaccaga gagttacatc ttcgctgact aatttgatct ggtacagcag
    11341 ttcattggga agatcgtcct gcgcggcgga aacggtggca cttccaccaa acagcaagcc
    11401 gacaataaca aatgtggaca ggataagttt catggcaaat ttctcctttt gttgaaagat
    11461 tgtccatatg ctgagacggc tttcatcgcg tacggatatg gcttcagcca ggaacctggc
    11521 ccgagcacgt gcagcaattt gagggtttcg ggcaggggcg ggcttcagtt catccagcag
    11581 ggcaattacc tgcgagtcaa attcgtcatc tttcaacatg ttcgacctca tcgttcaaaa
    11641 gaattttctg caggctcgcg agggcgcggt gttgtaaaga cttgaccgcg cccaccggtt
    11701 tattcaaaat atgcgcaatc tcttcattct cccagccctc aagaaatttg agcgcaatga
    11761 cttgctgctg atccgacgtc aattcatgaa tggcggcgcg cacacgaagt ttgcggatgt
    11821 tttgatcggc ggtttcttca atgttaccga aaccagatga atcgtcgttg agcagtagta
    11881 ggggtgactg gcggcggtaa tgatccacaa caaggttgtg gatcgtgcga tagagatagg
    11941 cctgcagaaa atcactcggc cctttacgcg ttcgtatggc ttgcaggaaa cgaccgaatg
    12001 tctctgctac acaatcctcg gcagtgcagg catcgcccag caaacgaact gcatagcgat
    12061 acaagcgcgg gctgtagaga tcgtaaattt cttccagggc gcagggatca agctgccggg
    12121 ctctcaacaa caaatcttgt tctgatatca ccgtgactca tttcttacga cgcttaatac
    12181 ggctggaaga tacgggagga aaaacaggaa tgcgtgattt cgctttgata tctgaatttt
    12241 tcgccatatc ggcagcctat tttcagtcta cctctcgcga gtaaagagaa tttgcaggat
    12301 ttgtaaagaa tcaataaagt tctatctcca tttttcttta ccgaatcttt acaggacttt
    12361 tgaatgccct ccataatctg aagctacact ctgggcaatc aattttccgg aggtcgttta
    12421 tggttacttt gtttggcaaa cgcatacgca tctttcgccc gcggacgatc tggatacgaa
    12481 agattgtcca atggttcttc tttttgctga tcgcactcat cgcggtcaat catacgctgg
    12541 cagaaaatgg cagagggatt cccatcctgt cgagcgcgtc gctgcacgca gtctgtccgt
    12601 ttggcggagt ggaaacgctg tacaccctgg cagcaggtgg aatactcgtc aagaagatcc
    12661 atgactcctc actggtgctc gcgggcatcg ttctggttct ctccattttg tttggtccgg
    12721 tcttttgcgg atgggtgtgc cccttgggaa cggttcagga atgggtgggc aagattggga
    12781 aaaagctctt tagacggcga tacaatcact ttgtgccggc gcgactcgat aacgttctcc
    12841 gctacgcacg ctatggcatc ttgctctggg tgctgtacat cacagccacc agcgggacat
    12901 tgatcttcga agcttacgac ccatactttg ccctgttcaa tttctggagt acagaggttg
    12961 catggagcgc catcgccatt ctcggcatta cgctcgtact ctcggtcttc gtggagcgac
    13021 cctggtgtaa atacgcttgt ccgtatggtg cggtgctggg aattacaaac ttgtttcgag
    13081 tgttctccat aaaacgctct gagaccacct gcaaggcgga cggcgcctgc tcgatcatgt
    13141 gccccatgaa catccccgtg gacacaaaga aagttgtccg cgatcatcag tgcatttcgt
    13201 gcctggaatg tacatcggaa gcaatctgcc cggtcgcaaa cacagtcttg ttcaccggtg
    13261 gaggtgtgaa atgacactca cttcaaaatc attggctgtc attgtgctgg cgatggtatt
    13321 cggtggaatt gcattctcat cggttatggg ttggtgggca actgaatcga ccaagcaacc
    13381 tgtcactttt gctgacggtg aatttgcagg gcaagcggac cctgcagata tccgcggttc
    13441 gtataccttt agcgatattg caaactcctt cgatgtgact cctgaggtac tggcgcaagc
    13501 ctttggcatt acagaaagtg atcccgccgc ttttgcagtg aaagaactcg aagcgatcta
    13561 ccttgatagc ggattcgaaa ttggtacagc atccgttagg ctgtttgttg cgttttattc
    13621 cggtctgcca ttcgatacaa ccgagcagga aatttacatg ccacaatccg cgacggacat
    13681 ct
//
LOCUS       DASTJP010000789         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_916028, whole genome shotgun sequence.
ACCESSION   DASTJP010000789 DASTJP010000000
VERSION     DASTJP010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916028"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFQ23_23165"
     CDS             complement(<1..222)
                     /locus_tag="VFQ23_23165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909567.1"
                     /translation="MNNDNEPVLTSSELLAYQRSIGGLPDFSPPRAVIFVPQKTLAEY
                     ALRRHPARRIKGFFGEFYLFKNTGGEVAIS"
     gene            complement(219..1427)
                     /locus_tag="VFQ23_23170"
     CDS             complement(219..1427)
                     /locus_tag="VFQ23_23170"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET9909568.1"
                     /translation="MFSASTLSLRLNSFWLLIARLTTQGLAVAFIALVARRLEPASFG
                     QFTFIATIVFIGNTFTSFGTDSLLIREVARSGQIPPLVHRALTLQLVLSALFCFALIM
                     FRVNAPLLIYSLSLFPLAIFSIVSALLRGLERMDLFWGISVAIGLLQILFALFASDLL
                     TLCILLLIGNSLTSFIAMRIFSFLEADSGLLPLLDFRPLLPIALPFAALTIFSVILQR
                     LGILSIWMLINDTATGQFSSAARILDGMKFGHYAVLGALLPMISRGSFRSQQNYRLAF
                     TGLFVLSILLAGVAAFFARPILLFLFGEEYISAVDILGILAWCIVPYTISAFISVDLV
                     ARGKETLLLRSIVISLLVSIVLYSWCIISFGVEGASWAALGGEIFQAVVLLLVRSKSV
                     IESEMMIHKK"
     gene            complement(1433..>2521)
                     /locus_tag="VFQ23_23175"
     CDS             complement(1433..>2521)
                     /locus_tag="VFQ23_23175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909569.1"
                     /translation="FPSDFKVPLVISIDLEQDGPNHGVRTVAESFDWQYGPKEIVFHK
                     EHIGLLQNFYYCGGLTSTYGSVIFLEDDSLVSPAFYQYAAESLSYFEEDPCVAGISLY
                     RYAFNGFTHQPFEPLADGSDIFFMQVSSIMGQAWSRRQWNAFEKWRVANKADSGNTNT
                     ALHDLWSKFPSDDHFPILTRYLVSANRYYVFPRVSLTTGFGDAGTHFAKRTSYFQVPL
                     QQSATAFRFKKMESSNSVYDSFMEILPVCIKRLAPDFQNLDFEVDLNATKDHRHLTAD
                     YVITTRISSNPLKSFALAMRPPEANVIFKAGGEGISLCRADDIRRDTWSELQTRKRLY
                     DYFSRGSQTSLRKSLLYFLMDLFPHRKR"
BASE COUNT          803 a          601 c          570 g          547 t
ORIGIN      
        1 ggaaatcgca acctctccac ccgtattttt gaataaatag aactccccaa agaacccctt
       61 gattctcctg gcaggatggc ggcgaagtgc atactctgca agagtttttt gaggtacaaa
      121 aatcaccgcg cgtggtggag aaaaatcagg aagccctccg attgaacgct ggtaagctaa
      181 caattcagaa gaagtcagga ccggctcatt gtcgttgttc attttttgtg aatcatcatc
      241 tcgctttcaa tcactgattt cgaacgaact aagagaagaa ctaccgcttg aaagatctca
      301 ccgccgagcg ctgcccagga ggcgccctcc acgccaaagg atatgatgca ccaagaatac
      361 aggacgatcg aaacaagtaa tgaaataaca attgatctta gtaataaggt ttctttaccg
      421 cgcgcgacca gatcgaccga aataaacgca gaaatcgtat atggcacaat acaccacgca
      481 agaattccta agatatctac cgccgagata tattcttcac cgaaaagaaa cagtaggatc
      541 gggcgcgcaa aaaatgcagc gactcctgcc agcaagatgc tcaatacgaa taaaccagta
      601 aaagcaaggc ggtagttttg ttgtgaacga aatgatcctc tcgatatcat gggcagtaag
      661 gcgccgagaa ctgcgtaatg cccaaatttc attccatcca gaatacgggc tgcagatgaa
      721 aattggcccg tcgctgtgtc gttgatcaac atccaaattg acaagatgcc caatcgctgc
      781 aaaatgacac tgaagatcgt taaagctgca aacggcaagg cgatgggcaa tagggggcga
      841 aagtcgagta gaggtagtaa gccagagtcc gcttcaagaa acgagaagat ccgcatcgca
      901 ataaaagagg tcagcgaatt tccaatcaac agcaaaatac aaagcgtcaa caagtcagaa
      961 gcaaacaaag caaacaatat ttgaagtaaa ccaattgcta cgcttatccc ccaaaacaaa
     1021 tccattcgct ccagccctcg taaaagtgcg gacacaattg aaaaaatcgc cagtggaaat
     1081 aggctcaaag agtaaatcaa caaaggcgca ttaacacgaa acattatcag agcaaagcaa
     1141 aaaagagcag aaagcaccaa ctgcagcgtc agtgcacgat ggacaagcgg cgggatctga
     1201 ccggacctgg cgacttcgcg aatcaacagg ctatctgttc caaaacttgt aaaggtgttg
     1261 ccaataaaca ctatggtagc gataaatgtg aattgaccaa aactggccgg ttccagccgc
     1321 cgcgccacca atgcgataaa cgccaccgcc aggccttgcg tggtcagccg cgcaattagc
     1381 aaccagaacg aattgagccg aagggatagg gttgacgcag agaacaagcg cttcaccgtt
     1441 ttcgatgagg aaacaaatcc ataaggaaat acagaagaga ttttcttaga ctcgtttgac
     1501 ttccacgcga gaaataatca tacaggcgtt tgcgagtctg cagttcactc catgtatccc
     1561 ggcgaatgtc gtcagctcga catagactaa tcccctcccc accagccttg aaaatcacat
     1621 ttgcttctgg gggtctcatc gcaagggcga acgatttcaa aggattggaa ctaatgcgag
     1681 ttgtgattac ataatccgct gtaagatggc gatgatcttt ggtcgcattc aaatcgacct
     1741 caaaatcgag gttctgaaaa tcaggtgcaa gacgctttat acagaccggc agaatttcca
     1801 tgaacgagtc atacacggaa tttgagctct ccatcttttt gaatcgaaat gcggtcgcgc
     1861 tttgctgaag cggaacctga aaataggaag tccgttttgc aaaatgggta ccggcatctc
     1921 caaagccagt ggtcagcgaa acacgcggaa aaacataata tcgattcgct gaaaccaaat
     1981 atcgcgtcag gatgggaaaa tggtcatcgc tcgggaactt cgaccaaagg tcatgtaaag
     2041 cggtgttggt atttccgcta tccgccttat tggctacacg ccatttttca aatgcgttcc
     2101 attgtcgtct actccaggct tgccccatga tcgatgacac ctgcataaag aatatgtcgc
     2161 tgccatctgc cagtggctcg aatggctgat gcgtaaatcc gttgaacgca taacgataaa
     2221 gtgagatgcc tgccacgcag ggatcttctt caaaataact caacgactca gccgcgtatt
     2281 gataaaacgc aggcgataca agtgagtcat cctctaagaa gatgacagat ccgtatgtgc
     2341 ttgtcagtcc gccgcaataa taaaaatttt ggagcagccc gatgtgttcc ttgtgaaaaa
     2401 cgatctcctt tggaccatat tgccaatcaa aggattcagc aacagttcga acgccgtgat
     2461 ttggtccatc ctgctccaaa tcaatggaaa ttaccaacgg cactttgaaa tcagaaggaa
     2521 a
//
LOCUS       DASTJP010000790         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1227051, whole genome shotgun sequence.
ACCESSION   DASTJP010000790 DASTJP010000000
VERSION     DASTJP010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227051"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFQ23_23180"
     CDS             complement(<1..641)
                     /locus_tag="VFQ23_23180"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909570.1"
                     /translation="MTTFRTSIPLKTYFFGLIVLLYLPIAILFLFSLNSNTILSFPLK
                     GLTLDWYQQLFVSDALLRSARNSLVVALGSSLAATFLGTMVALLMMRYQFRSKGFLVA
                     LAALPLIVPYIVLAVALFLLFSVLKIDRSLWTVGIAHTIVALPYVLLIVGARLAGFDS
                     SIEEAAMDLGADYPTTLRRIVIPMITPAMISAWLTAFTVSFDEFALALFLAGT"
     gene            complement(638..1567)
                     /locus_tag="VFQ23_23185"
     CDS             complement(638..1567)
                     /locus_tag="VFQ23_23185"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909571.1"
                     /translation="MTTPTRPSITRPHFLKKMGAGRGRGLDRRATFWWLYIPPLFWLG
                     AFFLIPLLLMVAYSFRESMSGDFFQLWQPSLKQYSQLFATGSYWRLLGTSAWTALLIS
                     LSAVVLSYPVAYFLAFHGRQRVGFYLVLLLVPFWTSYLLRVMAWKIMLGTEGVINSFL
                     LYINVIQEPLTALLYNRNAVLITLIYVWIPFATLPILAALQRIDTSLFEAAADLGARP
                     VHQFLRVTLPLSLPGVFAAFFMVFIPTIGEYVTPLLVGGSRGSLYGNIIQDFFTKAAN
                     WPFGSAMSMVMLAATLVLVAIAMRLINLRDLLE"
     gene            complement(1564..>2185)
                     /locus_tag="VFQ23_23190"
     CDS             complement(1564..>2185)
                     /locus_tag="VFQ23_23190"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9909572.1"
                     /translation="DGVDSWDALMDPALAGHISMWDDGPGAVTISSYIHGYDETNITA
                     EQLDAIKAEWIAQRDLNLFYWAGEPELVEGMTSGDVWAAYAWQGAYATLLANGVPVAY
                     ANPKEGRNSWVGLYGIRKGTENLDLALKFLDAKLGEATGNNVVNLFYYGHTNQDVMNG
                     ITDETLKQAFSIDDPSILEKTNFTPNLTAEQRDAWTAMWAEVKAAP"
BASE COUNT          659 a          552 c          531 g          443 t
ORIGIN      
        1 tgtgtgcctg ctaaaaataa tgccagagcg aattcatcaa atgaaacagt aaaagcggtt
       61 agccaggcag agatcatggc cggcgtgatc ataggaatta cgatacgccg aagcgtagtg
      121 gggtaatctg ctcccaggtc catcgcggct tcttcaatac tggaatcaaa cccggcaagc
      181 cgcgccccca cgatcaatag cacatacggc aatgccacaa tcgtatgcgc aatgccgacg
      241 gtccataatg accgatcaat cttgagtaca gaaaaaagca gaaacagggc cacggcaagt
      301 acgatatagg gaacaatcaa cggcaatgca gccagcgcca ccagaaatcc cttactgcgg
      361 aactggtaac gcatcatgag caaggcaacc atcgttccca ggaacgtagc agccaaactg
      421 ctccccagag cgacaaccag actattgcgt gcggagcgta gcaatgcatc cgaaacaaat
      481 agctgttggt accaatccag ggtcaaaccc ttaagcggga aggaaaggat cgtgtttgag
      541 ttgagagaaa acaaaaagag aattgcaatc ggaagataaa gcagtacgat caagccgaaa
      601 aaataggtct taagaggaat cgaagtgcga aacgttgtca ttcgagaaga tccctcaaat
      661 tgataaggcg catcgcgatt gcaacgagta ctaatgttgc agcgagcatg accatagaca
      721 tcgctgaacc aaagggccag ttggcagctt ttgtgaaaaa atcctggatg atattgccat
      781 acaacgaacc gcggcttccg cccaccaata gtggagtcac atattcgcct atagtgggaa
      841 tgaataccat gaaaaatgca gcgaagaccc ccgggaggct caacggcaaa gtaacccgta
      901 gaaactgatg gacaggacgt gcccccagat cagccgcagc ttcaaaaagt gatgtatcga
      961 tccgttgaag cgcagcaaga attggaaggg tcgcaaacgg aatccaaaca tagatcaacg
     1021 taattaaaac tgcgttacgg ttatagagca atgcagtcaa cggttcctga atcacattga
     1081 tatacagcag gaaggaattg atcactccct cggtacccaa catgatcttc caggccatca
     1141 cccgtaaaag ataactcgtc caaaacggga ccagcagcaa gacaagataa aaccctacac
     1201 gctggcggcc atgaaaagca aggaaatagg cgacgggata ggatagcaca acggcggaaa
     1261 gtgaaattaa taaagctgtc caggcagagg ttcccagtag acgccaataa cttcctgtgg
     1321 caaacagttg tgagtattgt ttgagagagg gctgccaaag ctgaaaaaaa tccccgctca
     1381 tgctctcgcg aaaactgtac gctaccatca gaagcagcgg aatcaaaaaa aatgccccaa
     1441 gccagaacaa gggggggatg tagagccacc aaaatgtggc gcggcgatcc agaccccgac
     1501 cccggcccgc ccccattttc ttaagaaagt gggggcgggt gattgaaggt cgggttgggg
     1561 tggtcacggc gcggctttca cctcagccca catggctgtc catgcatcgc gctgctccgc
     1621 tgtcagattt ggtgtgaagt ttgttttttc caagatggaa ggatcatcaa tcgaaaaggc
     1681 ctgcttcagc gtttcatcgg taatgccatt catcacatcc tgatttgtat gtccataata
     1741 gaaaagatta acgacgttat tgcccgttgc ttcacccagt tttgcatcca ggaatttcag
     1801 agccaggtcg agattctctg ttcctttacg aatgccataa aggcccaccc atgaattacg
     1861 cccttctttc gggttggcat atgcgactgg caccccattt gcaagcaggg ttgcatacgc
     1921 accctgccac gcataggccg cccatacatc gccactcgtc ataccttcga cgagttccgg
     1981 ttcaccggcc caataaaaga gattgagatc acgttgagca atccattctg ccttaatcgc
     2041 atccagctgt tctgcggtga tattggtttc atcatatcca tgaatatagg aggaaattgt
     2101 cactgcacca gggccgtcat cccacatgga gatatgtcct gccagtgccg gatccattaa
     2161 cgcgtcccat gaatcaacac catct
//
LOCUS       DASTJP010000791         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1431166, whole genome shotgun sequence.
ACCESSION   DASTJP010000791 DASTJP010000000
VERSION     DASTJP010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431166"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /locus_tag="VFQ23_23195"
     CDS             complement(<1..1598)
                     /locus_tag="VFQ23_23195"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9909573.1"
                     /translation="MLLRSLKSQIQKYERIKTLMVNQELEKLLATLDNDLPRSALFKN
                     KVPALAKAFDAEQMKQSLQAALFDENDDRYSIVKCVPGKALYLNDHVINMQFKLTIQD
                     KASNETTETMVNARLFQELDACKTYLDQILEPIARRMEGRPEIQPFARPVAMVEPLSM
                     TLSVFPIDGMIPTLVDTTDPKTVAGILSKTLPDAISGEFAIENVQLLLAHYGRYKRCV
                     LRYSVDGKNTATQSPQNETVYGKIDADGLGSQIGPIIAALRVALQKPDLLYRFRVPRS
                     LGYLPDLQLLLMEALPGKPFFKPLLKAWIENNNHQTGANESLPHEEDARLDKAIQTCA
                     LIAATLHSSDIKLGRQTTLEMQVAKLQESADVISQVYPELGAQINSWIQQTVDFAKAY
                     PAMPLCFSHGDFTYTQLIFDGKEGGLVDFDTMCQAEPAQDLGHYLAYQRLNIIKDQDP
                     NAPFAPEAIEHLCALFLDTYIEVSKAWIPDEAQLRGRVAIYELISLIRLTIHSWEKMK
                     GSRLKQTIPLLEERIEWQKQISQSV"
     gene            complement(1549..3486)
                     /locus_tag="VFQ23_23200"
     CDS             complement(1549..3486)
                     /locus_tag="VFQ23_23200"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909574.1"
                     /translation="MSKKDKKKDSDDKKSGSSSLTFRRLVGTIVRFSGNSRRNFFLAA
                     FMLIFEQVAATAVGWVAPGLVLDHVGERLAEMRVNPAAAAFTSEDFRVLTIVTVSLIA
                     LTMLNSLCDSLAEIYLAQGGRKVGYNMRVFLYSHLQKLSLSFHSQSRTGDILTRVTSD
                     VAAVENFIIGNLSDFVGSVLALIVRMAAIFWIAWQVGVVALIIIPLMGLISNYFSNLI
                     KSASKKLRSSEGELASAAQEMLASIRVMQVYGQGDYEQSMFSGQSQKAMNAALEAATY
                     QARFSWVVSVFGAISTSAVIWVGTWLVFGRPLQMQVGDLSRFIIWIDEMFKPSKRLIS
                     EWNAFGKLYASLERINDLIELKPDVQDKPGAIAVDSLRGNIEFRNVVFSYPSVETSKK
                     KGDAKPRPTLNGLSFTIKPGQVIALVGHTGAGKSTVAQLIPRLYDPSGGQILIDGRDI
                     REYTLESLRAQMSMVLQESILFTGTIVENIAYGRPDATGMEIIEAAKDANADEFISKF
                     EDGYYTLLAERGANLSGGQRQRISIARAFVRDTPILILDEPSTGLDAESTDLVLQALR
                     KLMKGKTTIIISHELNLIRDADQIIVIKEGQVEQIGTHDELLRSGGLYANLYIMQSGQ
                     RALDNDAATVSEIANPEVRKD"
     gene            complement(3535..>3741)
                     /locus_tag="VFQ23_23205"
     CDS             complement(3535..>3741)
                     /locus_tag="VFQ23_23205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9909575.1"
                     /translation="VAGIPELVEHNRNGFLYQQHDVDGISLGITELLRDAEKRAQLGA
                     AALKKVTEQFDVRLAADRLKALFV"
BASE COUNT          981 a          957 c          850 g          953 t
ORIGIN      
        1 actgattggc tgatctgttt ttgccattcg attctctcct cgagcaaggg gatggtttgc
       61 tttagacggc tgcctttcat tttctcccaa ctatgtatgg taagccggat gagactgatc
      121 aattcataga tcgcgacgcg ccctcttagt tgtgcttcat cgggaatcca cgctttggac
      181 acctcaatgt acgtatccag aaacagagcg cataaatgct caatcgcttc cggagcgaag
      241 ggagcatttg gatcctgatc cttaataata ttcaatcttt gataggcaag ataatgaccc
      301 aaatcctggg ctggttccgc ctggcacatg gtgtcaaaat ctaccagacc tccctctttg
      361 ccatcaaaga tcaattgagt atatgtaaaa tcgccatggc taaaacacaa aggcatggca
      421 ggatacgctt tggcaaaatc gacggtctgc tgaatccagg aattgatttg tgcacccagc
      481 tcaggataaa cctgggaaat gacatcggca gattcctgca atttagcaac ttgcatctcc
      541 aacgtggttt gccgccccaa ctttatatcg gagctatgaa gggtagcggc gatcagagcg
      601 caggtctgaa tcgctttatc gagccttgcg tcttcttcat gcggaagact ctcgtttgcg
      661 cccgtctgat ggttgttatt ttcgatccag gctttcaaca ggggtttgaa aaatggttta
      721 cccggaagag cctccatcaa cagcagctgt aaatctggaa gataccccaa cgaacgaggc
      781 acacgaaatc gatacaggag gtctggtttc tgcaatgcaa ctcgtagcgc agcgatgatc
      841 ggaccgattt ggctgcctaa cccatccgca tcgatcttgc catacaccgt ctcattctgt
      901 ggagattgcg tcgctgtgtt tttaccatca acggagtaac gcagaacaca acgtttatac
      961 cgaccgtaat gcgcgaggag aagttgcaca ttttcaatgg cgaactcccc ggatattgcg
     1021 tccggtagag ttttggataa gattcctgca acggttttgg gatcagttgt atcgaccaac
     1081 gtaggaatca tgccgtcaat ggggaataca gataacgtca tgcttaaggg ctcgaccatt
     1141 gctacaggtc tggcaaatgg ctggatttcc ggacggcctt ccattcgccg ggcaatcggt
     1201 tccagaattt gatcaaggta cgttttacac gcatcaagct cctggaaaag tcgcgcgttg
     1261 accatcgtct cggtggtttc gttactcgcc ttatcctgaa tcgtcagctt gaattgcata
     1321 ttgataacgt gatcattcag gtagagtgct ttaccaggaa cacacttgac aattgaatac
     1381 cgatcatcat tttcgtcaaa cagcgcagcc tgtaacgatt gcttcatctg ctcggcgtca
     1441 aaggcttttg ccagtgcagg gactttattc ttaaagagcg cgctgcgcgg caaatcatta
     1501 tccagagtcg cgagtaattt ttccaattcc tgattcacca tcagcgtttt aatcctttcg
     1561 tacttctgga tttgcgattt cagagaccgt agcagcatcg ttatccaggg ctctctgacc
     1621 cgattgcata atgtataagt tagcatacaa cccgcctgag cggagcaatt catcgtgcgt
     1681 gcctatttgc tcgacctgcc cttccttgat gacaataatc tgatcggcat cgcgaatcaa
     1741 gttaagctca tgggagatta ttatagtggt cttgcccttc attagtttgc gtaaggcctg
     1801 aaggacaagg tcagtcgatt ctgcatccaa gccggtggaa ggttcgtcca gaatcagaat
     1861 aggcgtatca cggacaaaag cacgagcgat agaaattctc tgccgctgcc cacccgataa
     1921 attggcgcct cgttctgcca acagggtgta atacccatct tcaaatttgg agataaattc
     1981 gtctgcgttg gcatctttgg cggcttcgat gatctccata cccgttgcat ccgggcgccc
     2041 gtaggcaata ttctcaacaa tcgtgccagt aaacagaatc gactcctgaa gcaccatact
     2101 catctgcgct cgcagggatt ctaatgtgta ttcgcgtata tcacgaccat caatcaaaat
     2161 ttgtcctccg gacggatcat acagtcgcgg aatgagctga gcaacagtgc tcttacccgc
     2221 tccggtatga ccgaccaaag caatgacctg acccggctta atagtgaacg aaagcccatt
     2281 caatgtggga cgcggctttg catctccctt tttcttcgaa gtttcaaccg agggataaga
     2341 aaaaactaca tttcgaaact cgatgtttcc tcgtaaggaa tctacggcaa ttgctcctgg
     2401 cttatcctga acatcgggtt tcaactcaat caggtcattg atgcgctcca ggctggcata
     2461 cagcttacca aacgcattcc attcagaaat cagtctcttt gatggcttga acatttcatc
     2521 aatccagata ataaatcggc taagatcacc gacctgcatc tggagcgggc gaccaaacac
     2581 gagccatgtc cccacccaaa taacggcaga tgtggagatg gcgccaaata cactgaccac
     2641 ccagctaaaa cgtgcctgat aggtggcggc ttcgagagcc gcgttcatgg ctttctggct
     2701 ctgtccggaa aacatggatt gttcgtagtc tccctgccca tacacctgca tgactcgaat
     2761 cgaagctaac atttcctggg cagctgacgc aagctcccct tcgctggaac gcagcttttt
     2821 gcttgcgctt ttgatgaggt ttgaaaaata attggagatc aaccccatca gcggaataat
     2881 gatcaaggca accacaccca cttgccacgc gatccagaat atagcagcca tacgaactat
     2941 gagagccagt acactgccga cgaaatcgga aagattaccg ataataaagt tttccacggc
     3001 ggctacgtct gaggtgacgc gcgtgagaat gtcacccgtc cggctttgac tatgaaacga
     3061 tagtgacagt ttttgcaaat gcgaatacag gaagactcgc atgttatagc ccaccttgcg
     3121 gccgccctgg gcaagataga tttccgcgag cgagtcacat aagctgttga gcatggtcag
     3181 agcaataagc gacacggtaa cgattgtcag tacgcgaaaa tcctcggagg tgaaagcagc
     3241 cgccgccgga ttgactctca tttcagccag ccgctcacct acatgatcca atacaagacc
     3301 cggtgccacc cacccaacag ccgtggcagc gacttgttca aaaatcaaca taaaggcagc
     3361 caggaaaaag tttcgccgcg agttaccaga gaatcggaca atcgtcccca ccaggcggcg
     3421 gaaggtgagg ctgctggaac cagacttttt atcatccgag tcttttttct tatctttttt
     3481 actcattagc acaccaatct aaatctaacg aaaaggatag tagccatcat aatatcaaac
     3541 aaaaagtgct tttaggcgat ctgctgccag cctaacatca aattgctcgg tcactttctt
     3601 caaagctgct gcacctaatt gcgcgcgttt ttctgcatca cgtaaaagtt cagtaattcc
     3661 caatgaaata ccatcaacat catgctgctg gtataagaac ccatttctgt tgtgctcaac
     3721 aagttctgga attccagcca c
//
LOCUS       DASTJP010000792         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1022231, whole genome shotgun sequence.
ACCESSION   DASTJP010000792 DASTJP010000000
VERSION     DASTJP010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022231"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VFQ23_23210"
     CDS             complement(<1..1028)
                     /locus_tag="VFQ23_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9909576.1"
                     /translation="MMASGKKGKMAAIKKRSASMMEKRTNNTLPRDAQMDDLLLENHR
                     AENPNEMLTEMNDLEGLVIMGDEVGDPDIGVLVQQEEELIDDVLDLKSLAVATELSED
                     PVRLYLGEIGRVKLLDADSEFRLATMIESNRLVASLRRHPLRKGLTLECAIYHELLTE
                     MQTSWERLLEDTERLQHQPPNLGLILDESQVLHAGWESSEPSYLRAFLDNGLWGIDEL
                     WNDIARKAYSVFLSLYLLPRNYANWLRENIGQKGQLPNQETLFQNLPSDEQLEQELNA
                     AEFRAVDANQSLIRANLRLVVSVAKRYLGRGIAFDDLIQEGNMGLLRAVGKFDPRRGF
                     KFSTYATW"
     gene            complement(1056..>2830)
                     /gene="dnaG"
                     /locus_tag="VFQ23_23215"
     CDS             complement(1056..>2830)
                     /gene="dnaG"
                     /locus_tag="VFQ23_23215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET9909577.1"
                     /translation="VVWPESGTWRCFGQCNEGGDVFKFVMKRENLDFKDALNTLAARA
                     GVEIPAYIKETPEQREAFDNLRKLLEDSLLFYRRHLLANTALLNYLHNQRGLTDASIE
                     TFGLGYAPNAWDSALTYFTQRGYTQRDMLEAGLISERDNGGYYDRFRNRIMIPIRDED
                     GKMAGFGARIIDPDDVPKFLNSPETPVFSKGRILYGLDRARKPIRSADQAVIVEGYLD
                     VIALHQAGYENVVSPMGTALTEDQLRLLKRFTRKVVLALDPDTAGQKAVLRGLDAARS
                     AMDREGELGFDAKGLLRNEARLQADLRVASLPDDLDPDEIVARDKNEWAQLIEKAKPI
                     VTHVMESLAAGQNISDPKVKNQIAAQVLPLIEDLPSALERDTYRQQLARMLRVDERSL
                     VGMKPEVPRIRREKRMQSVQKQAVEKSAVVVSVSSNKKVEAYIIGVLFEKPALLYRLD
                     RFLQEYGLIPLTAHDFEYTDYQMLFGLIRESLEQDKTEQHEFVVEAVPESLQGLSREL
                     VAQAEKQERIDDKMLEELLRGVIKLRRVAAGENLNQLRFLQEEAHQGGDLRAASYQDL
                     VLQHTRLLRDLDQASRKMSLKRMD"
BASE COUNT          680 a          778 c          639 g          733 t
ORIGIN      
        1 caccaggtgg cataggtact gaatttgaaa ccacgccgcg ggtcaaactt acctacggcg
       61 cgcaggagcc ccatatttcc ttcctgaatg agatcgtcga aggcaatgcc gcgccctaaa
      121 tagcgtttgg caacactaac caccagacgc aggtttgcgc gtatcagcga ctgatttgcg
      181 tcaacagcac ggaactcagc agcattcagt tcctgttcca attgctcgtc tgagggaagg
      241 ttttgaaaga gtgtctcctg gttcggtaat tgtccctttt ggccgatatt ttcgcggagc
      301 caatttgcat aatttcgcgg cagcagataa aggctcagaa agacactgta cgcctttctt
      361 gcgatgtcat tccacaactc atcgatgccc cacagcccat tatccagaaa cgcacgcaga
      421 taagaaggct cactcgattc ccagcctgca tgcagcactt gtgattcatc aagaatcaaa
      481 ccaagattgg gaggctgatg ttgaagccgt tccgtatctt cgagtaagcg ctcccaggag
      541 gtctgcatct cggtcagcag ctcgtgatag atggcacatt caagagttag tcccttacga
      601 agcggatgac gacgcaggct tgccacaaga cgattggact cgatcatggt cgcaagacga
      661 aattcactat ctgcatccag aagtttcaca cggccgatct cccccagata aagccgcacc
      721 gggtcttcag acagctctgt agccaccgcc aggcttttca ggtcaaggac atcatcaatt
      781 aattcctctt cctgttgtac aagaacgccg atatcagggt cacccacctc atctcccata
      841 attaccaaac cttccaaatc attcatctcg gttagcattt catttgggtt ttccgcgcga
      901 tgattttcca acaacagatc atccatttgc gcgtcgcgcg gcaatgtgtt gttcgttcgt
      961 ttttccatca ttgatgcgga tcgtttcttt atggcagcca tcttcccttt cttaccagac
     1021 gccatcatgc aaaaattgtt acgtcccttt taaaatcaat ccatccgctt taaagacatc
     1081 ttccgagatg cctggtccaa atccctcaaa agccgtgtat gttgtaacac cagatcctgg
     1141 taggaagccg cgcgcaaatc tccaccttga tgagcttcct cctgtagaaa ccgtagttgg
     1201 ttcaaatttt cgccagcagc tacacgacgt aatttaatca caccgcgcag cagttcttcc
     1261 agcatcttgt cgtcgatgcg ctcctgtttt tcagcctgtg ctactagttc ccgcgaaagc
     1321 ccctgcaacg attcaggtac agcttcgaca acgaactcat gttgttcggt cttatcctgt
     1381 tccagtgatt cgcgtatcaa tccaaaaagc atttgatagt ctgtgtattc aaaatcatgc
     1441 gcagtcaaag ggatcaatcc gtattcctgc aaaaacctat ccaggcgata gagcagcgca
     1501 ggcttttcga atagaacgcc gataatatac gcttctacct ttttattgga cgaaaccgac
     1561 acgactacgg cagacttttc aactgcctgt ttttggacac tttgcattcg tttttcacgt
     1621 cgtatgcgcg ggacttcggg cttcatccca accaacgagc gttcatccac ccgaagcatt
     1681 ctcgccagtt gctgacgata cgtatcacgc tccaaagcgc tgggaagatc ttcgatcagc
     1741 ggcaggacct gtgccgcaat ttgatttttt accttgggat cgctgatgtt ttgtcctgct
     1801 gcgagggact ccatgacatg cgtgacgatt ggctttgctt tctcaatcag ctgtgcccat
     1861 tcatttttat cgcgcgccac aatttcatcc gggtccaaat catccggcag ggatgcaaca
     1921 cgcaaatccg cctgcaggcg ggcctcattt cgtaataaac cttttgcatc gaagccaagc
     1981 tcgccttcgc gatccattgc cgatcgcgct gcatccagac cgcgtaacac agctttttga
     2041 ccggcggtgt ccgggtccaa ggcgaggacc accttgcgcg tgaagcgttt caacaggcgc
     2101 aattggtctt ccgtcaatgc ggtgcccatg ggtgagacaa cattttcata tcccgcttga
     2161 tgcagcgcga tcacatccag atagccttca acgatcacag cctggtcggc agaacgaatt
     2221 ggtttacgag ctcgatctaa tccgtacaat attcgccctt ttgaaaagac cggtgtttca
     2281 ggagaattca gaaattttgg aacatcgtcc gggtcaatga tacgggcgcc gaacccagcc
     2341 attttcccgt cttcatctct gatcggaatc atgatcctgt ttctgaaacg gtcgtaataa
     2401 ccgccgttat ctcgttcaga gatcaaacca gcctccagca tatctcgctg ggtatacccg
     2461 cgttgggtga aataggtcag ggctgagtcc catgcgtttg gcgcgtagcc cagaccaaag
     2521 gtctcaatgg atgcatctgt tagtccgcgc tggttatgga gataattaag caatgcggta
     2581 ttcgcaagca gatgacgtcg atagaacagc aacgagtctt cgagaagttt tcgaaggttg
     2641 tcgaatgcct ctcgttgttc aggggtttcc ttaatatagg cagggatttc cacacccgcg
     2701 cgggcggcca gcgtgttcaa tgcatccttg aaatcaaggt tttcacgctt cattacaaac
     2761 ttgaagacgt cgccaccttc attacactgc ccaaagcagc gccatgtgcc agactcgggc
     2821 catacgacaa
//
LOCUS       DASTJP010000793         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1349405, whole genome shotgun sequence.
ACCESSION   DASTJP010000793 DASTJP010000000
VERSION     DASTJP010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349405"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VFQ23_23220"
     CDS             <1..779
                     /locus_tag="VFQ23_23220"
                     /inference="COORDINATES: protein motif:HMM:NF021622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909578.1"
                     /translation="YSVNSPKNQIIRSTLTRLIQTGQFGPDVDQANALRQRLRHLTSS
                     LDGIDLIELTSDLIHRQQLGRNDNDYRLMLEICWLILQRQMPTELAGEHPLPVLDRDA
                     LVLHHIYERFVANFYRAHLNGWGVAPQKRLEWHEKNTNPYLPSMIPDVVLQERSSGEL
                     IILDTKFTAHSLTPNPWGKEIFDSSHLYQLYAYLKSQAHLSEAHRNASGILLYPVVHK
                     NFSEKIELPDHLMRVESVDLSLPWSDIERQLIELINPSEF"
     gene            945..1622
                     /locus_tag="VFQ23_23225"
     CDS             945..1622
                     /locus_tag="VFQ23_23225"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET9909579.1"
                     /translation="MMLREKASPDISEFLRYLAANEEAENGLPTLNELSQELGVSVAS
                     LREQLEVARALGLVEVKPGRGGTRRRTFSFTPAIRQSLGYALALGNGHFQKYSELRNH
                     VESAYWYEAVQKLTEEDKEELQALVSRAWEKLRGSPIQVPHEEHRSLHLLFYSRLENP
                     FVTGILEAYWDAYEAMRLNMLTGGYEYLEEVWQYHQQMVEAVCNNDLKAGYDALITHT
                     DLLYHRP"
     gene            1740..>2644
                     /locus_tag="VFQ23_23230"
     CDS             1740..>2644
                     /locus_tag="VFQ23_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET9909580.1"
                     /translation="MDKVVNDFSIIVGTKNGSGSSTANTTILRAIFKMGIPVGGKNLF
                     PSNIQGLPTWYTIRVSKDGFLARREEQEIVVAMNPDSFTRDLASVVPGGAFYYADDIK
                     LPITRADIAIYPMPVKQLVRSDQSIPSDFRDQVGNMIYVGVLSHMIGLDVETIRAALT
                     FHFKGKQKPIDMNMNAIKMGAEWASANLEKKDPFYLEHMDKTEGLIMADGNTAAAIGS
                     IFGGAQFVAWYPITPASSLAESVIDYFPMLRKRDDGKFDYAVVQAEDELAAIGMAVGA
                     GWSGLRAMTSTSGPGLSLMTEFAGLA"
BASE COUNT          658 a          635 c          672 g          679 t
ORIGIN      
        1 aatatagtgt caattcgcca aagaatcaaa tcatccgcag tacgctcacg cggctgattc
       61 aaaccggtca attcggaccg gatgtggacc aggcaaacgc cctccgtcaa cgcctgcgcc
      121 atttgaccag tagcctcgat ggaattgatt tgatcgaatt aacatcagat ttgattcatc
      181 ggcagcaatt gggacgcaac gataacgact atcggcttat gctggaaatc tgctggctca
      241 tcctgcagcg tcagatgcca actgagttag ccggtgaaca tcctcttccg gtcttggatc
      301 gggatgcgct ggtcttacat catatctatg agcgatttgt ggccaatttt tatcgcgcac
      361 atctaaatgg atggggtgtc gcaccccaga aacgccttga gtggcatgag aaaaatacaa
      421 atccgtatct gccgtcaatg attcccgatg tcgttctaca ggaaaggtct tctggcgagt
      481 tgatcatttt ggataccaag tttaccgctc acagcctgac cccaaatccg tggggcaagg
      541 aaatctttga ctcatcgcat ttgtatcagc tttacgcgta cctgaaatca caggcacatc
      601 tctccgaagc acaccgcaat gcttcaggaa tcctgcttta ccctgtcgtt cataagaact
      661 tttcagaaaa aatcgaactg ccagatcact taatgagagt ggagagtgtt gatctcagtt
      721 tgccctggag tgatatcgaa aggcagttga ttgagttgat caatccttct gaattttgaa
      781 cctgttattt ccaattgcat tttcatacga taaatctgaa ttccttgact tcgccttcaa
      841 atcgtcatat actcgaatta atcggattaa ttggagttac gacattgcgc atccaggata
      901 aagatcgctt tcagtcgaaa gtgaatcgat aagagacgag cattatgatg ttgcgagaaa
      961 aggcatctcc tgatatttct gagtttcttc gttatctggc tgcgaacgaa gaggcagaga
     1021 acggtctccc cactctgaat gagctgagcc aggagctcgg ggtcagtgtt gcttcgctgc
     1081 gtgagcagct tgaagtggcg cgcgcgctgg ggctggtcga agtcaaacct gggcgtggag
     1141 ggactcgtcg tcgtactttt tcgttcacgc cagccattcg tcagagtctg gggtacgccc
     1201 tggcgcttgg aaatggtcac tttcaaaaat attccgagct gcggaatcac gtagagtccg
     1261 cctattggta tgaagctgtt caaaaattaa cagaagaaga taaagaggaa ttgcaagcat
     1321 tggtttctcg cgcatgggaa aaattgcgcg gctcacccat tcaagttccg catgaagaac
     1381 atcgcagttt gcacctattg ttttatagca ggttggagaa tccgtttgtg accggcatcc
     1441 tggaagccta ttgggatgcg tatgaggcaa tgcggctgaa tatgctcacc ggcggttatg
     1501 agtatttgga agaagtttgg cagtatcatc aacagatggt ggaagcggtt tgcaataatg
     1561 acttgaaagc cgggtatgat gcgctcatca cgcatacgga ccttttgtat catcgaccgt
     1621 aattattttg tcattgcgag ccccgagcga aagcgagtgg gcgaagcaat ctccaaatta
     1681 ataaggagat tgccacgtcc agtctcggac tctcctcgca atgacgtact aggagcatta
     1741 tggacaaagt agttaacgat ttttcgatca ttgtgggaac caagaacggt tcaggtagtt
     1801 cgacggccaa tacgaccatc ctgcgcgcta ttttcaagat gggcattccc gttggcggaa
     1861 aaaacctctt cccctccaac attcaaggct tacctacctg gtataccatt cgcgtcagca
     1921 aggatggttt cctcgcccga cgcgaggagc aggaaatcgt cgtggcaatg aaccccgact
     1981 cattcacgcg cgaccttgct tccgttgtgc cgggcggagc gttctattac gcggacgata
     2041 ttaaactgcc gatcacacgc gccgatattg ccatctaccc gatgccagta aaacagcttg
     2101 tccgcagtga tcaaagtatt ccatctgatt tccgcgatca ggttggcaac atgatttacg
     2161 tgggcgttct ctcccatatg atcgggcttg atgtcgaaac cattcgcgcc gcgttgacgt
     2221 tccacttcaa agggaagcag aaacccatcg acatgaacat gaatgccatt aagatgggtg
     2281 ccgagtgggc atcggctaat ctcgaaaaga aagacccgtt ctatctcgaa catatggata
     2341 agacggaggg tctgatcatg gcggatggta ataccgcagc cgcgatcggt tcgatttttg
     2401 gcggtgcgca atttgtcgcc tggtatccga tcacccctgc ttcatcgctc gctgaatctg
     2461 ttatcgatta cttccctatg ctgcgcaaac gggacgatgg caaattcgat tacgcggttg
     2521 tccaggcgga agacgaattg gctgccatcg gtatggcggt cggcgctggc tggagtggtt
     2581 tacgcgccat gacctccact tccgggccgg gcttgagcct gatgacagaa tttgcagggt
     2641 tggc
//
LOCUS       DASTJP010000794         5911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1444934, whole genome shotgun sequence.
ACCESSION   DASTJP010000794 DASTJP010000000
VERSION     DASTJP010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444934"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1872)
                     /locus_tag="VFQ23_23235"
     CDS             complement(<1..1872)
                     /locus_tag="VFQ23_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909581.1"
                     /translation="MPDLNSIQLQIAEAPLESKIFVSGPNGTGKTTTGVERMRYLLAQ
                     GVPADSILMLTPQRILQEPYLDLLFSPERGAGGEATSTTMGGLARRIVELFWPIASEA
                     AGFASPDQPPVFLSLETSQYYMAHIVRPLLDEGYFESVTIDRNRLYSQILDNLNKAAL
                     IGFPHTEIATRLDSSYFGDPVQRRVYADAQDAANRFREYCLEHNMLDFSLQMEVFSNI
                     LWPLTPVRDYLTRSYRHLIYDNVEEDSARAHDILWDWMPDFESALLLFDPSGGFRYFL
                     GADPGTGRVLSERCDEQIEFQESFVSSESIAYLRQGLIEAIDPSFESKVESQKPDGTP
                     LAFIQSRFYPELLDGIVNEVETLIVDHNTRPSDIVILSPYLSDALRFSITNRLEARGV
                     PWRSHRPSRSLRDEPSSLALITLAMVGHPHWNIHPPKFDIAYALMLSIEGLDLVRAQL
                     LADIVYRQRDLSLSSFENIKPDVQERITFSVGNRYTILRDWLLAYREGMPLPLDHFFR
                     KLFGEVLSQAGFGYHSNFDAVRVAGSLVESVKNFRRAMEPSIVNKDRPDFDMGREYIA
                     MLEDGVIAASYVESWKSEDKDAVLVSPAHTFLMMNRPVTYQFWIDPGSNGWYQRLTQP
                     "
     gene            1975..2442
                     /locus_tag="VFQ23_23240"
     CDS             1975..2442
                     /locus_tag="VFQ23_23240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909582.1"
                     /translation="MPTPIRPTAKASHLAAKPGSIVKFSDQLTNSLNDITRMINEHKS
                     MIDTIQEVALELTNSIGTLHTLTVKYAGIANKILDGLLPIARGLPIIPKNILQMLINL
                     ESITQKIIDNNLKTAKTITDVQTGLKTGDVNKIKGHAGELQNVTKALTAILPK"
     gene            2671..3351
                     /gene="rplU"
                     /locus_tag="VFQ23_23245"
     CDS             2671..3351
                     /gene="rplU"
                     /locus_tag="VFQ23_23245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET9909583.1"
                     /translation="MRFAIVESGGKQYRVVEGRAIEVDRLPVEAGKKFDLERILLMSD
                     GDDVLVGTPLISDILVKVTVVDHVKGPKIDRFKYRPKKRIRVRGGHRQHYTRLMVDFI
                     GKPDEERKAEAPKQAEAKKETDDLVKIEGIGPKVSKTLKDAGITTFEALANTSVEDIQ
                     KILSDAGLKMMDATSWPEQAKLAAAGDWDGLKAMQDELSGGRAASQAKKPAAKKSATT
                     KKTDTKKK"
     gene            3363..3629
                     /gene="rpmA"
                     /locus_tag="VFQ23_23250"
     CDS             3363..3629
                     /gene="rpmA"
                     /locus_tag="VFQ23_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002088814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET9909584.1"
                     /translation="MAHKKGGGSSRNGRDSNSQRLGVKRYAGQFVLSGNILIRQRGTK
                     IKPGLNVKAGKDDTLFATADGVVMYDILRGQKRVNIVPEVEAEA"
     gene            3631..3837
                     /gene="rpmE"
                     /locus_tag="VFQ23_23255"
                     /pseudo
     CDS             3631..3837
                     /gene="rpmE"
                     /locus_tag="VFQ23_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            3889..4098
                     /locus_tag="VFQ23_23260"
     CDS             3889..4098
                     /locus_tag="VFQ23_23260"
                     /inference="COORDINATES: protein motif:HMM:NF015102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HET9909585.1"
                     /translation="MRPIEELGLATRAVESLKKVGIENVGQFLERLSQGNEAVLAIPG
                     FGQASLTAAKKKLRSLGYELPEAAA"
     gene            4255..4824
                     /locus_tag="VFQ23_23265"
     CDS             4255..4824
                     /locus_tag="VFQ23_23265"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET9909586.1"
                     /translation="MKHTQGSIEVVCGSMFSGKTDELIRRLVRATIAKQKVQVFKPAI
                     DIRYAVEKVTSHAGSNFDAIPVEKADDIRTNLDKDATVVAIDEAQFFDPEVIKVAQEL
                     ASQGVRVLIAGLDTDFRGEPFGPMPILMSMAEDVDKLHAICMVCGGEASRTQRLVNGK
                     PARYDDPVVIVGASELYEARCRLHHEVPR"
     gene            4883..5440
                     /gene="hpt"
                     /locus_tag="VFQ23_23270"
     CDS             4883..5440
                     /gene="hpt"
                     /locus_tag="VFQ23_23270"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET9909587.1"
                     /translation="MQHFENILAEVLISEEMLQTRVKELGAEISTDYPDNNLLLICIL
                     RGGVPFLVDLSRHIKSPHMVDFMAVASYGVGKRESSGAVRVTMDLQMDIHDKDVLLVE
                     DIVDSGHTIASVLELLQVRQPRSLKVCALLDKAGRREAVVPIHYRGFEIPNKFVFGYG
                     LDLDEYYRNLPFVGVVDLEKYKPST"
     gene            5465..>5911
                     /locus_tag="VFQ23_23275"
     CDS             5465..>5911
                     /locus_tag="VFQ23_23275"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HET9909588.1"
                     /translation="MSKESQAISAYAGRWVARVRGRIVAQGGTPEQALQAAKKIRHKE
                     RPEIIYIPFPYSYSPLVDRVSEILLHQEIYLVGGAVRDMMLNRLSDDLDFALPSNGIS
                     TARRVANALNADFMALDEERDTGRVIVAEADGERTFLDFATYRGVNL"
BASE COUNT         1626 a         1442 c         1499 g         1344 t
ORIGIN      
        1 cggctgtgtg aggcgctggt accatccgtt agagcccggg tcgatccaga attgatatgt
       61 cactggccga ttcatcatca ggaacgtatg ggccggtgag acgagcaccg catctttgtc
      121 ctcgctcttc caggattcca cgtacgatgc ggcgatcacg ccgtcctcca gcatggcgat
      181 atattccctg cccatgtcaa aatcagggcg atccttgttc acgatcgaag gttccatggc
      241 tctgcgaaaa ttcttcacag actcgaccag gctccccgcc acgcgcacag catcgaaatt
      301 gctgtgatat ccaaaacctg cctgtgacaa cacctctccg aataattttc ggaagaaatg
      361 atcgagaggc aatggcatgc cttcccggta agcaagaagc cagtctcgca atatcgtata
      421 tcggttgcca acactaaacg tgatgcgttc ctgcacatca ggcttgatat tctcaaaaga
      481 ggaaagggac aaatcacgct ggcgataaac aatatcagcc agcaattgcg cacggacaag
      541 gtcaagcccc tctatggaca gcatcaaggc ataggcaatg tcaaattttg gcgggtgaat
      601 attccaatgt gggtgcccta ccatcgccag cgtgatcaat gcgagggagg aaggttcatc
      661 tcgtagagac cgcgaagggc gatgcgaacg ccaggggacc ccgcgtgctt caagtcggtt
      721 agtaatggaa aagcgtagag cgtctgaaag atagggtgaa aggatcacaa tatcagatgg
      781 acgtgtgttg tgatcgacaa tcagcgtttc aacttcattt acaatcccat ccagaagttc
      841 cggataaaaa cgggattgaa tgaaagccag tggagtccca tcaggcttct ggctttcgac
      901 cttggactca aaactgggat caatggcttc aatgagtccc tgtcgtaggt aggcaattga
      961 ttcgctcgat acgaacgatt cctgaaattc aatttgttcg tcacagcgtt cgctcaacac
     1021 tctaccggtg cccgggtccg caccaaggaa gtagcggaat ccgccgctcg ggtcaaagag
     1081 gagcaaagca gattcaaaat cgggcatcca gtcccagaga atatcatgcg cacgagcact
     1141 gtcttcttcc acattgtcat agatcaaatg acgataactg cgcgtcaagt aatcacgcac
     1201 aggagttaac ggccacagga tgttactaaa aacttccatt tgcagcgaga agtccagcat
     1261 attatgttcc aggcaatatt cacggaaacg gttcgccgcg tcctgtgcat cggcatagac
     1321 acgtcgctgc accgggtcac caaagtacga agagtcaagt cgtgttgcaa tttctgtgtg
     1381 agggaaacca atgagcgcgg ctttattcag attatccaga atttgcgaat acaaacgatt
     1441 ccggtcaatc gtgaccgatt caaaataccc ttcatcgagc aacggtcgga caatatgtgc
     1501 catgtaatat tgcgaagtct caagggagag aaagacaggc ggctggtccg gactggcaaa
     1561 cccggcagct tccgaggcga tcggccaaaa taattcgacg attcggcggg cgagcccacc
     1621 catcgtagtt gaggtggctt caccaccggc gccacgttca gggctaaaaa gcagatcgag
     1681 ataaggctcc tgcaaaatcc gttgcggcgt aagcatgaga atcgaatcag ctggtacgcc
     1741 ctgggcaagc agataacgca tccgctcgac acccgtagtg gtcttccccg taccgttggg
     1801 gccggagaca aagatctttg attcaagagg agcttcagca atttgaagtt gaattgagtt
     1861 aaggtcaggc atgagatgag ggtacaggaa gtggaaaggt ttgtcaatat gattttgttg
     1921 aattcaatcg taatctcaag tatagttacg aaaattcata tcaaggagaa tctcatgcct
     1981 acccctatca gacccaccgc caaagccagc catctggccg ccaagcccgg atccatcgtc
     2041 aaattttcag atcaactcac aaactcatta aacgatatta ctcggatgat caatgaacat
     2101 aaatcgatga ttgataccat tcaggaggtt gcacttgagc ttacgaattc catcggcaca
     2161 ttgcatacac tcactgtcaa gtatgcgggc attgccaata agatccttga cgggcttctc
     2221 cccatagcca gaggcttacc catcattcct aagaacatcc tacaaatgct tattaacctt
     2281 gagagcatca cacagaagat catcgataac aatttaaaaa ccgcgaaaac aatcacggat
     2341 gtgcagaccg gtttgaaaac tggggatgta aacaagatca aaggccatgc cggcgaacta
     2401 caaaatgtga caaaagccct gaccgctatc ctaccaaaat aaaaggtttc gtttcctacc
     2461 gcgccaaaga ccgtttccgt tttttagtca aagcctcgtt gacacatctc caccctgcat
     2521 gtataatacc gtccgctact gcctcagcag attaacgctg aggcggttgt ttgtcgaaag
     2581 acaacgagaa accccagctt ccccgttcgc aggcgacgag tgaaagaact ccgcaggcac
     2641 acggcgaagt gaaagactgg agaaaaaata atgagatttg caattgtaga aagcggtggg
     2701 aaacaatacc gcgtcgtcga agggcgcgcc atcgaggtag accgcctccc cgtggaagca
     2761 ggtaaaaaat ttgaccttga acgcatccta cttatgtcag atggggatga tgtgctggtt
     2821 ggaacaccct taatcagcga catcctcgtc aaagtcactg tggtggatca tgtcaagggc
     2881 cccaaaattg accggttcaa atatcgacct aagaagcgca tccgtgtacg tggcgggcac
     2941 cgtcagcatt acactcgctt gatggtggat ttcatcggta aacccgatga agagcgaaaa
     3001 gctgaagctc cgaaacaggc cgaggctaag aaagagacgg acgacctcgt caagattgaa
     3061 ggcatcggtc cgaaagtttc gaaaacgttg aaagatgctg gcattaccac atttgaagcc
     3121 ctggcgaaca caagcgtcga agatattcaa aaaatcctga gtgatgctgg cttaaagatg
     3181 atggatgcga caagttggcc cgagcaggcg aaactcgccg ccgcaggtga ctgggatggt
     3241 ctgaaagcca tgcaggatga actatccggt ggtcgcgcag ccagccaagc aaagaaaccg
     3301 gccgcgaaaa agtccgctac aacgaagaag acagacacga agaaaaagta agagaggtag
     3361 aaatggcaca taaaaaaggt ggcggctcaa gccgcaacgg acgagactca aattcccagc
     3421 gattaggcgt caaacggtac gctggtcaat ttgtattatc tggcaacatc ctgatccgcc
     3481 agcgtggcac gaagatcaag cctggtctca atgttaaagc gggcaaggat gataccttat
     3541 ttgcaaccgc agatggcgtc gtcatgtacg acatcctccg cggacaaaag cgcgtgaaca
     3601 ttgttccaga agtggaagca gaggcgtaac atgcgagccg atattcatcc aacttactat
     3661 cctgatgcca aggtgacttg tgcgtcctgc ggcagaacgt ggacaaccgg ctccactaaa
     3721 aaagaaattc gtgtggatat ttgttccaac tgccacccgt tctttaccgg tgaagcctcc
     3781 cgtatcctcg acattgaagg tcaggtagat cgcttctaca agaagctttc tgttcgccag
     3841 acctacgtcg aacagcagaa ggcaaaggaa gaattgaagg gctctcctgt gcgcccgatt
     3901 gaggaactcg gactcgctac tcgtgcagtg gagtctctca agaaggtagg cattgaaaac
     3961 gtcggtcaat tcctcgaaag acttagccag ggcaatgaag ctgtgcttgc tattccgggt
     4021 ttcggtcagg catccctgac cgccgccaag aaaaagctcc gctcgcttgg ttatgaactt
     4081 cctgaagccg cagcataacc aatttgcaga ataaataatc tcaggtaaac ttacaggcag
     4141 gtgtgaaagc atctgcctgt tttgttaccc cctccgtcat gacatgacac ctcccccatt
     4201 ttccgactga agtccgtcgg aaaatggggg aggcaggatg ggggcaggag aattatgaaa
     4261 catacacaag gttccattga agttgtttgt ggttcgatgt tcagcggtaa gactgacgag
     4321 ttgatccgcc gactcgtccg agcgacgatt gccaaacaga aagtgcaggt tttcaagcca
     4381 gccatcgaca ttcgctatgc tgtcgaaaaa gtcacttcac atgcggggtc gaacttcgat
     4441 gccattccgg ttgaaaaggc tgatgatatt cgtacaaacc tggataagga tgcaacggtc
     4501 gtggcaattg acgaagcgca attcttcgac cccgaagtta taaaggtggc tcaggaactt
     4561 gcttcacaag gcgtgcgcgt gcttatcgct gggctggata cggacttccg tggtgagccc
     4621 tttggaccca tgcctatatt gatgtctatg gcggaagacg tagataaatt acacgccatc
     4681 tgcatggtct gcggaggtga agcttcaagg acacagagac tcgtcaatgg caagcctgcc
     4741 cgctatgatg atccggttgt gatcgtcggt gcatcggaat tatatgaagc acgttgtcgc
     4801 cttcatcacg aagtaccgcg ttagcggtgc gaagtgccgc gttaatttag cagtctttta
     4861 gttcacacat cgacgaaata ccatgcaaca ctttgagaat atccttgcag aggttctaat
     4921 ttctgaagaa atgcttcaaa cccgcgtaaa agagctcggc gcggagatca gcacagatta
     4981 tcctgacaat aatttgctgc ttatctgcat attgcgtggc ggagttccat ttcttgtaga
     5041 cttgagccgc catatcaaat ctccgcatat ggtggacttt atggctgttg cttcctatgg
     5101 tgtgggcaag cgtgaatcga gtggcgcagt gcgggtcacc atggatctgc agatggatat
     5161 ccatgataaa gatgtgcttc tggtagaaga tatcgtggac agtggccaca ccatcgcttc
     5221 tgtattggaa ctgcttcagg ttcggcagcc gcgcagcctc aaggtctgtg cattgctcga
     5281 taaagccgga cggcgagaag ctgtggtgcc gattcattac cggggattcg agatacctaa
     5341 taagtttgtc tttggttatg gactcgatct tgatgaatat tatcgtaatc tgccatttgt
     5401 cggtgttgtg gacctggaaa aatacaaacc atccacttaa tccaggattg aggtttggat
     5461 attgatgtcc aaagagtctc aagccatttc agcctatgcc ggccgttggg tcgctcgcgt
     5521 ccgcggcagg attgttgctc aggggggtac acctgaacaa gccttacaag ctgcaaagaa
     5581 gataagacac aaagaaagac cagaaattat ttacatccca tttccatatt cttattcacc
     5641 tttggtggac agggtcagcg aaatcctcct acatcaggaa atatatttgg taggcggtgc
     5701 agtgcgggat atgatgctaa accgcctctc agatgacctt gactttgctt tgccatcgaa
     5761 cggcatctcc acggcccgac gcgttgccaa cgcgcttaac gcggatttta tggcactcga
     5821 cgaagaacgc gatacaggac gcgtcatcgt tgccgaagct gatggtgaac gcacttttct
     5881 ggatttcgct acctatcggg gagtcaatct g
//
LOCUS       DASTJP010000795         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_767885, whole genome shotgun sequence.
ACCESSION   DASTJP010000795 DASTJP010000000
VERSION     DASTJP010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767885"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1268)
                     /locus_tag="VFQ23_23280"
     CDS             complement(204..1268)
                     /locus_tag="VFQ23_23280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9909589.1"
                     /translation="MYNLDQIAAEKDEMRAAVLNARAYKPIYVKIKVNYGCNLKCEMC
                     KHWRETREPPISMDRFRDIITELGELGCKKIHFSGGEPMLRPQLPDLVEHATQLGMRV
                     TLTTNGTLIDKVKAKRLIEAGLRGVNVSIDSPLRKMHEKIRGVEGSFKLTARAVSLFN
                     KYSHKGKLTVRINTVVGRSNYLTLATLPDLAHELGADGINLIPVDDHCGEHLSMRKKD
                     IQIFNSEIAPVIEQRANELGVSLADEDAYPFGRDDTEVKLGRAGRYAFGYYNKLPCYA
                     PWTHSLIDFNGLVYVCCMTREQIKPLGDVRKQSFREIWEGAAYRQIRLNMHPPALKPC
                     QRCDDFILENKKIWETIGPY"
     gene            complement(1288..1683)
                     /locus_tag="VFQ23_23285"
     CDS             complement(1288..1683)
                     /locus_tag="VFQ23_23285"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HET9909590.1"
                     /translation="MHKRTTPKIFKRAKELRREMTHQERKLHSHLRAHRTGDVHFRPQ
                     HAIGNYIVDFCAPRRKVIIELDGSQHLEQDAYDMERTAFLESKGYRVLRFWNNDVTNN
                     LASVLTVIWEALKEQEAKGSETSKAAGSN"
     gene            complement(1958..3139)
                     /locus_tag="VFQ23_23290"
     CDS             complement(1958..3139)
                     /locus_tag="VFQ23_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909591.1"
                     /translation="MAQVTSTVETNITPTKIATSIPTATAKLTCPKINSNIQFILPSD
                     NVDLEISILNYLNEGGDPTKIEPKASESEIPPLYALSADIDGDLLSEVVISVGNFFGD
                     PTIIHLYHCEQNDYQLAKAFTLDDISFAKPEFATKIFNTEPHFMILRSGYISGWRQDF
                     LAVGWQNSEWRLIRLAAGTTPSVIVLLDQNADGTKEIFVKTRTAATPGGGISRIIIDS
                     YSWNGNGFAFIKSDMPPGSDRVHYLDDAETAWRNGNPFLAISYYEIAARDPNLSSYWT
                     TYELGTNQTELAAAYQKAFAFFRIVAIWFYLDRPDRASEYIQEMAETFREGKPGSEFV
                     LAAKAISESYDKEPSFSKACSQAVNFLDTKYPDVVRNHLGDWGVANPIYSATSDICKF
                     E"
     gene            3383..>3940
                     /locus_tag="VFQ23_23295"
     CDS             3383..>3940
                     /locus_tag="VFQ23_23295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9909592.1"
                     /translation="MRLKADLLLFMVAIIWGTAFVAQGVAGQYKVAYLFNGVSFMLAA
                     LILIPFTLKGAGSTKITSEQWKWMLIAGVVLFIATAMQQVGLLYTKVANASFLTSLYA
                     VFTPFLLWIGFREKPHRLDTIAVGMAAVGAFFLSTAGKFEVQRGDSLEVIGSIFWALH
                     FVVIGKFASRFESVSFASGHFFISGL"
BASE COUNT          973 a          919 c          910 g         1138 t
ORIGIN      
        1 tcaatcgcgc cagcatgtgg gatgatgaag accgcgcaga tatgttttct gatttgagtt
       61 aatccgttgg tcgagtagcg ttgagcgatg gttgcgaagc gcatatcgag accaacattt
      121 tcaagaaatt atctgttcat atcttacttc tggtctcggt acatgcctcg acttaccact
      181 cggcattact cgaccagcga gtgctaatat ggaccaatcg tctcccaaat ctttttattc
      241 tccaggataa aatcatcgca gcgctgacag ggcttgagcg cgggcggatg catattcaaa
      301 cggatttgcc gatatgccgc gccttcccag atctctctaa acgattgctt tcgcacatca
      361 cccagcggct tgatctgttc gcgcgtcatg cagcacacat aaaccaaccc attgaagtca
      421 ataagactat gcgtccacgg cgcgtagcag ggaagcttgt tgtagtagcc aaacgcataa
      481 cgacctgcgc gccccagctt aacctcagta tcatccctgc caaacggata agcatcttcg
      541 tccgcgagac tcacgcctaa ctcattcgca cgctgctcga tgactggcgc gatctcagaa
      601 ttgaagattt ggatatcctt cttccgcatc gagagatgtt caccgcaatg atcatctacc
      661 ggtatcaaat tgatcccgtc tgcgccgagt tcatgcgcca aatcgggcaa ggtagcaagc
      721 gtaagatagt tggatcgccc gaccactgta ttaatgcgga cggtcaactt gcctttgtga
      781 gagtatttgt tgaacagtga aaccgcgcgt gccgtgagct tgaacgaacc ttccacgccg
      841 cgaatttttt cgtgcatttt gcgaagggga gaatcgatcg agacattcac ccctcgtaag
      901 ccagcttcaa ttaatcgttt cgctttgact ttgtcaatca atgttccgtt ggttgtgagt
      961 gtgacacgca tcccgagttg ggtggcatgt tccacaagat caggcaactg cggacgcagc
     1021 atcggctcgc cgcccgaaaa atggattttc ttacagccga gttcgcctaa ttctgtgatg
     1081 atgtctctaa agcgatccat cgaaatgggc ggctcgcgcg tttctcgcca gtgcttgcac
     1141 atctcgcatt tgagattaca tccgtagttg accttgattt tgacgtaaat gggtttataa
     1201 gcgcgtgcat ttaaaacagc ggcgcgcatt tcatcttttt cggcggcgat ttgatcgagg
     1261 ttgtacatta agtggtcttt gttttcatca attactacct gctgctttac tcgtttctga
     1321 tcccttggct tcttgttcct tcaaagcctc ccagatcacc gttaagacgg acgccaaatt
     1381 atttgtcaca tcgttattcc aaaaacgcag aactcgatag ccttttgatt ccaaaaaagc
     1441 agttcgttcc atatcgtaag catcctgttc caaatgctga ctaccatcca gttcaataat
     1501 cactttgcgg cgcggggcac aaaagtccac tatgtaattc ccgatagcgt gttgcggacg
     1561 gaaatggaca tcgccagtgc ggtgggcgcg taaatggcta tggagtttgc gctcttgatg
     1621 tgtcatctcg cggcgcaact ctttggcgcg tttgaagatt ttgggggtgg tacgtttatg
     1681 cattcgaccc cctccctgcc ctcccccaat tgattaacac aattggggga ggagcgattc
     1741 ttcgttcgga ggtgaaaaat atgttcataa ctgcttttca ttccccttcc cccaaatccg
     1801 ctggttgatt tgggggaagg acgggaaggg ggtcaattag gcgcatttca tctttttcga
     1861 tggcgatttg gtcgaggtta tacattaagt actcagaaca gaatgaaaag ttgtttccat
     1921 tgtaacattc gacaattagt gcttgctatt ttaagtttca ttcgaacttg caaatatcag
     1981 acgttgcaga atatatcgga tttgcgacac cccaatcacc taaatgattt cttaccacgt
     2041 ccggatactt tgtatctagg aagttaacag cctgggaaca ggccttggag aagcttggct
     2101 ctttatcata cgattccgaa attgctttgg ccgccaaaac aaactcgctg cctggttttc
     2161 cttctcgaaa ggtctctgcc atttcttgaa tatactcaga ggctcgatca ggacggtcaa
     2221 gatagaacca tattgcaaca atgcggaaga aggcaaaagc tttttgatac gctgcggcta
     2281 actcggtttg gttagttcct agctcatacg tggtccaata actactaagg ttggggtctc
     2341 gtgccgcaat ttcataataa gagattgcga gaaatggatt accatttcgc catgctgttt
     2401 cggcatcatc caaataatgg actctgtcac tacccggtgg catatcactt ttaatgaaag
     2461 caaacccgtt tccattccaa gaatatgaat caataattat tcgacttata ccgccccccg
     2521 gcgttgcggc tgttctggtt tttacaaaga tttcctttgt gccatctgca ttttggtcca
     2581 atagaacaat tacagaggga gtggtgcccg ctgccagacg gatgagccgc cattctgaat
     2641 tctgccaacc aaccgctaaa aaatcttgac gccagcctga aatataccca gaacgcaaga
     2701 tcatgaagtg tggttctgtg ttaaaaatct ttgttgcaaa ctctggttta gcaaaagaaa
     2761 tatcatcaag cgtaaatgct ttcgccaact gataatcatt ttgttcacag tgataaaggt
     2821 gaatgattgt tggatcgcca aagaaattac cgacacttat aacaacttcg gacaataaat
     2881 caccatcaat atcggctgat aaagcgtaaa gaggtggtat ttctgattcg ctggctttgg
     2941 gttcaatttt cgttggatcg ccaccttcat tcagataatt caggatcgat atttcaaggt
     3001 ctacattatc cgaagggaga atgaactgaa tatttgaatt gattttgggg caagtcagtt
     3061 ttgcggtcgc agtaggaatg cttgtagcaa tttttgtggg ggttatattt gtctctacgg
     3121 tactggttac ctgagccaag actgtgtttg tttgagttga cggaactgga tgttgcgcgg
     3181 gcacacaggc agacattaca attaatatta aggcaagata ttgcttacat gattgcttca
     3241 tttcttagat tacctaggat ttcttgataa cggacttttg tgattagtgt cagatcttaa
     3301 gtggcgcact gtataattga ctatatacaa cattataaag tttcccccgc agttagacat
     3361 taaaacataa tttcaccttc ccatgcgcct caaagccgat ctcctcctct tcatggttgc
     3421 catcatctgg ggcacagcat ttgtcgcgca aggggtggcg gggcagtata aagttgccta
     3481 cttgttcaac ggtgtgagct ttatgctcgc ggcattgatc ctgattccat ttaccctgaa
     3541 aggggctggc agcacaaaaa taacaagtga acaatggaaa tggatgctca tcgcgggcgt
     3601 cgttttattt atcgctacag ccatgcaaca ggtgggcctt ttatatacaa aagttgccaa
     3661 tgcgagcttt ctaacaagtt tatacgcggt cttcacgccc ttcctgttgt ggatcggttt
     3721 ccgcgagaaa ccgcaccggc tggatacgat tgccgttggt atggctgcgg tcggtgcgtt
     3781 ctttctgtcc acggcgggta agttcgaagt ccaaagaggc gactcgctgg aagtcatcgg
     3841 ctcaatcttc tgggcgttgc attttgtcgt catcggtaag ttcgcatcca gatttgagtc
     3901 cgtttcgttt gcgtccggtc actttttcat cagcggcttg
//
LOCUS       DASTJP010000796         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_387948, whole genome shotgun sequence.
ACCESSION   DASTJP010000796 DASTJP010000000
VERSION     DASTJP010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387948"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFQ23_23300"
     CDS             complement(<1..492)
                     /locus_tag="VFQ23_23300"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9909593.1"
                     /translation="MRKFILIIVIFFTAALVYLSFGELESIVKTLRQANLGFLLLAFL
                     IQSGWFVVSGFTYLSLYRLLNMESSLYKLSMLFAASYFVSTVTPSAGMSGIVVFLTDA
                     NRNGQSPGKITVISVLYIFLDYLAFLFMLTLGLIVLFRRNDLDPSEIAASFVILVIAV
                     GLGS"
     gene            complement(600..1259)
                     /locus_tag="VFQ23_23305"
     CDS             complement(600..1259)
                     /locus_tag="VFQ23_23305"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HET9909594.1"
                     /translation="MIPAFILMAATSWYVRHANSKWSQIRASSGLTGAQAAQRLISTG
                     NLYGVQVQRTPGELTDHYDPRNKTLFLSQTVADVPSVAAVAIAAHELGHALQDAEEYF
                     PMKIRSALVPAVNIGSNLGWFLIIIGLVLGITQLAWVGVIVFASGALFALATLPVEFN
                     ASARAKQLLYETGIIQTEEERGGVNQVLNAAALTYVAGLVTAVLQLLYYVNLVGGRSR
                     D"
     gene            complement(1311..2618)
                     /locus_tag="VFQ23_23310"
     CDS             complement(1311..2618)
                     /locus_tag="VFQ23_23310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9909595.1"
                     /translation="MNQGLKTFVNIMAGIILLAGAFAGGVLVGHFVPSSSQLPFLSDL
                     IPSAPTMTPDEQTATPNELETLFAPFWEAWNIVHNEYVDQPIDDVTLMRGAIRGMMEA
                     LGDKQTFYMDPEVYDTQMALLQGDEYEGIGAYVDTDGEYLKVISPISGSPADQAGLRP
                     GDEIIAIDGEDMTGIPPEQARQKVLGPAGTTILLRIRREGESAPLEVSITRARIAVKS
                     AEGKMLQDGIAYIDINTFGDNTTSELRTALDSVLVENPRGIIIDLRNNGGGYLHTSVE
                     VASEFISDGVVLYEQYGDGRRDVYDATGNGRATDLPIVVLVNEGSASASEILAGALQD
                     YERAKLVGVQSYGKGSVQIWFPLSDDEGAARVTIAKWLTAKERAIDGVGLTPDVYVEC
                     SECSLVNTDNDVQVQTAIETLLAMINNSPIPTSMPTTIPTPVQ"
BASE COUNT          750 a          709 c          719 g          655 t
ORIGIN      
        1 cgaaccaagc cccacagcga ttacaagaat tacaaacgac gctgcaatct cacttggatc
       61 caggtcgtta cggcggaata agacgatcag ccccagggtc agcatgaaaa ggaaggcaag
      121 ataatccagg aaaatataca gcacgctgat gacggtgatc ttgccaggcg attgtccgtt
      181 tctgttggca tcggtgagga agaccacgat tccgctcatc cctgcgctgg gggtaactgt
      241 gctgacgaag taggatgccg caaacagcat ggagagttta taaagtgaag actccatgtt
      301 caacaaacga tagagggaaa gataggtgaa tccggaaacg acgaaccaac cactctggat
      361 cagaaatgca agcagaagaa accctagatt tgcctgacgg agtgttttta caatgctttc
      421 gagttcgccg aaactcaggt aaaccagtgc cgcggtaaaa aagataacaa tgatgagtat
      481 gaacttccgc atatcggcag gattatacac aaacacccgc gagatcctcg cgggtgtcat
      541 caaaaagact tcggaagcgc cagagaatgg caggacttac gaagtctgat cggtcatgat
      601 taatctctgc ttcgtccgcc gacgaggtta acgtagtaaa gcaactgcag gacggcagtc
      661 accaggccag ccacataggt cagcgcggca gcgttaagga cctgattgac accgcccctc
      721 tcttcctcgg tctgaatgat gcctgtttca tacagaagct gctttgcacg tgcagaagcg
      781 ttaaactcta caggcagcgt agcgagtgcg aaaagagcac cgctggcgaa gacgatcacg
      841 cccacccagg ccaattgcgt tataccgagc accagaccaa tgatgatgag gaaccagcca
      901 aggttggagc cgatgttgac agcaggcaca agggccgaac ggattttcat tgggaaatat
      961 tcctcagcat cctgcaaggc atggccgagc tcatgggcgg cgatcgccac agcagcaact
     1021 gatggaacat cggcgacggt ctgcgagaga aataaggttt tattgcgcgg atcgtagtga
     1081 tcggtgagtt caccgggcgt tctctggacc tgcacgccat acagattgcc ggtcgagatc
     1141 aatcgctgcg cggcttgcgc gccggtgagt ccgcttgaag cgcgtatctg gctccatttg
     1201 ctattggcgt gacgtacata ccaggacgtc gcagccatca aaatgaaagc cgggatcatg
     1261 aagatgaagt agagtgggtc aaagaaaaac atgatttacc tccgggttga ttattgaaca
     1321 ggggttggaa tagtggtggg catcgaagtc ggaatcgggc tattattgat catagcaagc
     1381 aaggtctcga tggcggtctg cacttgtaca tcgttatcag tgttcacaag actacattca
     1441 ctgcactcta catagacatc gggcgtaagg cccactccat ctatggcgcg ctctttagcg
     1501 gtaagccatt ttgcaatcgt cacacgcgct gcgccttcgt cgtcagaaag cgggaaccaa
     1561 atctgtacag agccttttcc ataggactgc acaccgacaa gttttgcgcg ctcataatcc
     1621 tgtaatgcac ctgctaatat ttcagaagca gaagcagagc cttcattaac taacactaca
     1681 ataggaagat ccgttgcacg tccgttgcca gtggcatcat acacatcgcg gcgaccatct
     1741 ccatattgtt catagagcac gacaccatca gagataaact cagacgccac ttctaccgag
     1801 gtgtgtaaat acccgccgcc attattgcgc agatctataa tgatgcctct gggattttcc
     1861 accagcacag aatccagtgc ggtgcgcaat tcagacgtgg tattgtcgcc aaaggtgttg
     1921 atatcgatat aagcaatacc atcctgtagc attttccctt ccgcactctt gaccgcaatc
     1981 cgtgcccggg taatgctaac ttccagcggc gccgattcgc cctcacgccg aatccgtaga
     2041 aggatggttg tgccagcggg tcccagcact ttttggcgtg cctgctcggg tgggatgccg
     2101 gtcatgtctt cgccgtcgat ggcgatgatt tcatcgcccg ggcggagacc ggcctgatct
     2161 gcgggagatc ctgaaattgg gctgattact ttaagatatt ctccatcggt gtccacatag
     2221 gcaccaatgc cttcatattc atcgccctgg agcaatgcca tttgtgtatc gtagacttcc
     2281 gggtccatgt agaaggtttg tttgtcaccc aacgcttcca tcatgccgcg gatcgcgccg
     2341 cgcatgagag tcacgtcatc aataggctga tctacatact cattgtgaac gatattccat
     2401 gcctcccaga atggggcaaa cagggtttcg agctcattgg gggtggctgt ttgttcatcc
     2461 ggggtcattg ttggggcgga agggataaga tcgcttagaa atggtaattg gctgctcgat
     2521 ggcacgaaat gcccaaccaa aacaccacct gcaaatgccc ctgccagcaa aattattccc
     2581 gccatgatat taacaaatgt tttcaaacct tgattcacta atgcctccgg aagataaaga
     2641 cagctaaata ttccacaacg atacaataca ctgattagta tacgctgaat taattgttac
     2701 ggatgtatat gagtcgcgta agcagattat atgttttcaa acgatttttt acccgtttta
     2761 ttcggtaggg atgacccgta taaaaatgaa agttcgggcg ctcgttcgat ttgcctgaca
     2821 aatcgtgtcg ccc
//
LOCUS       DASTJP010000797         6983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_388075, whole genome shotgun sequence.
ACCESSION   DASTJP010000797 DASTJP010000000
VERSION     DASTJP010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388075"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFQ23_23315"
     CDS             complement(<1..445)
                     /locus_tag="VFQ23_23315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909596.1"
                     /translation="MSKASLVIENLSFQYRTRSELAIKGISFELQPGEMILIAGSSGC
                     GKTTLARCINGLIPRSYRGERTGKVLLQGTDVSEMQIADVAQVVGTLLQDPERQIVAS
                     NVYNEIAFGPENLGLPREEIGRRVENALKRLNMEDLRDRQTFNLSG"
     gene            complement(450..1247)
                     /locus_tag="VFQ23_23320"
     CDS             complement(450..1247)
                     /locus_tag="VFQ23_23320"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET9909597.1"
                     /translation="MSVITLMTDFGIKDGTVGVMKGVIWGICLTAQISDLSHLIQAQN
                     IREAAFILARSISYFPKGSVHVVVVDPGVGTARRPMAAQIGDWYYVGPDNGTITGLLE
                     QAEQAGWRTDFVELDRKEYWLENISSTFHGRDIFSPVAAHLANGVALEKVGTAFSNPV
                     RIEVARPQKMDHGWHGEVNHIDHFGNVSTNIRVEHLGEKMTAKENIMVRLNGIEIKGL
                     VKTFGERPVGEIIALIGSTGYLGISIVNGNAAEKLGVKTGDEIFLNW"
     gene            complement(1311..2432)
                     /locus_tag="VFQ23_23325"
     CDS             complement(1311..2432)
                     /locus_tag="VFQ23_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909598.1"
                     /translation="MMESFFPTLVLIAGIVVVFLTLSSALSTFVLPRAARSQLNRLVF
                     GLLRRIFDFFAHFAKSYIRRDAIMAYYAPIGLMLLLPTWYVLILVGYAAIYWAVGFGD
                     LFDVFRLSGSSLFTLGFEISKTPLVTLLAFSEAMIGLMLVGLLIAYLPTMYSAFARRE
                     QVVNMLEVRAGSPPSALEMLLRFNRIHGLEKLSDYWKTWEAWFADVEESHTTLPALIF
                     FRSPRPENSWVTAAGTVLDAAAITLSAVEIPYEASAALCIRAGYLSLRRIATYFDIPN
                     PQDPHYPEHDICVEREEFEDVLNQLAIAGLPIKEDRDQAWRDFAGWRVNYDRALILLC
                     GLVMAPHAPWSSDRAPQLKLPPLFILRKQRLLAEGSQSR"
     gene            2497..3105
                     /locus_tag="VFQ23_23330"
     CDS             2497..3105
                     /locus_tag="VFQ23_23330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909599.1"
                     /translation="MNQLLHHAVLTSFEIMVQLQTYTTIDFPAALKCQTLSFLRIQWP
                     GGFIGENRLRDWVTKESDHPIHFVLVEAGILISHTNVVWKYLEHEGMSYKAYGLTGVF
                     TYPAFRGEGYGSQIIEAGTNYIKQSDADIAMLYCDDSLHNFYAQHGGIPMDDSISYID
                     SEAGPVLVEDEILMMQFISPKGQQNRISFERQPIYFGSDSTW"
     gene            3115..3516
                     /locus_tag="VFQ23_23335"
     CDS             3115..3516
                     /locus_tag="VFQ23_23335"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET9909600.1"
                     /translation="MMSQKQLERVRNICTQLPETTERLSHGEPTFFVKNKVYVMFANN
                     HHNDGHIAVWLPVPSGFQSTLIEAAPQTYFMPPYVGVRGWVGIELAQISDPDLTYHIQ
                     VAWELTAPKRLLSKVGQLNASQPDRKGEIHK"
     gene            3513..3815
                     /locus_tag="VFQ23_23340"
     CDS             3513..3815
                     /locus_tag="VFQ23_23340"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HET9909601.1"
                     /translation="MSFSKLTGLSLIVLQLTFNAVFFLLQRTFEYPDILRKPTDYILS
                     RFNQGGRQLIMTWYAFAFTGVLFVPVAFLVHQTFASENTPFMAFATTIGLLAGVSE"
     gene            3821..4501
                     /locus_tag="VFQ23_23345"
     CDS             3821..4501
                     /locus_tag="VFQ23_23345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909602.1"
                     /translation="MTKAIDIYLEIGRKRTFAGAIDWPGWCRSGANTESALQALVEYG
                     PRYARVLRLEKIEFKPPADVSVFSIVEKLEGNATTDFGAPGLAPSADSELVNEEELQR
                     FEAILRACWKAFDSAVKSAQGKELLKGPRGGGRQLDGVIKHVLEADEGYLSSLGWKIH
                     KNDKFDQSEALKFRRKEILEGLAASAHGEIPPRGPRGGLRWTPRYYVRRVAWHVIDHV
                     WEIEDRIN"
     gene            4511..4825
                     /locus_tag="VFQ23_23350"
     CDS             4511..4825
                     /locus_tag="VFQ23_23350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909603.1"
                     /translation="MEDEVLIRRGNTLVRRLQLEAGEAMPWHRDPFHRVTVILQGSAI
                     AIEFRDGSKEERFTVRAGQVDWDEPTDRLHRGVNVGDEPYEEITVFFLKHSDDVPQPV
                     EE"
     gene            4895..5389
                     /locus_tag="VFQ23_23355"
     CDS             4895..5389
                     /locus_tag="VFQ23_23355"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HET9909604.1"
                     /translation="MLINPELVNAINAQIGREFGASMQYLSIAGYFHAQKLTLIAKLF
                     FEQAEEEKMHAMKFVHYVLDTQGELQIPMIAAPTASFANAEEAVQAALTWETEVTGQI
                     NQLMDLAVSQNDYLAQNFLQWFVDEQLEEVTKMDQLLSVVRRAGERNLLMVEAYLIHI
                     DKAG"
     gene            5597..6961
                     /locus_tag="VFQ23_23360"
     CDS             5597..6961
                     /locus_tag="VFQ23_23360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909605.1"
                     /translation="MRFTPWFKQFFLVFCLLSISLAACIPTSGTAVSTSTPSPAPSLP
                     LTGEPAATAAPIIGSGLRVAFVKERNIHLWDESTGQSQIIFNAGDVIAVKMSEDGQLI
                     AFLRRSAVQLTEAEWREQSSLWIVDSNGDNPRELVSADALRQRLNAVERDSTNIPQLE
                     WVPGTHKLLFSGWTYFVMAEGESHAVPQGLFLIDADSLTDAELVPAGNNLRFVPSLDG
                     NQIALMSLNGLSFINTDGGNLRPNVLTYTQVGRTAPLFPTGVWTQDSTAFLVTGSLEA
                     DPEFNINFTIWRVPVDGSAQTSLATITKSDPRSVMFSPDGQRIAFIQPTDTNPPFSAS
                     WFIMALIGNASPIAIQPSFEVWSASLHWSPAGDPFTGTLKKLCPDASNDGNICDDRLY
                     FDGALSAIHWIDSNRVLFLTRNPSVLFLATMDFTSPWDATTIPIVAWPLNESVSLNSF
                     TTAK"
BASE COUNT         1831 a         1770 c         1711 g         1671 t
ORIGIN      
        1 cgccagatag attaaacgtc tgacgatcgc gcaggtcctc catattcaaa cgtttcaatg
       61 cattttcaac tcgccggccg atctcttcac gtggaagacc caggttctca gggccgaagg
      121 cgatctcgtt atagacgttg cttgctacga tctggcgttc cgggtcctgc agcagagttc
      181 ccaccacctg agccacgtcg gcgatctgca tttctgaaac atctgtgcct tgcaacaaaa
      241 ccttccccgt acgttcccct cgataggaac gggggatcag cccattgata caacgcgcca
      301 acgttgtttt gccgcaaccg ctggagcccg cgatcagaat catttctcca ggttggagtt
      361 cgaatgaaat gcccttgata gcaagttcag aacgagtacg gtattggaag gataggtttt
      421 caattactaa agaggctttg gacatcacat caccaattca aaaaaatttc atcaccggtc
      481 ttaacaccca acttttccgc agcgtttcca ttaacaatcg agattcccaa ataacctgta
      541 cttccgatca aagcaatgat ttcgcccacc gggcgttcac cgaatgtttt gaccaaaccc
      601 ttgatctcaa ttccattcaa gcgcaccatg atattttcct tggcggtcat cttctcgcca
      661 agatgttcaa cccgaatatt ggtggacaca ttgccaaaat ggtcaatgtg attcacctcg
      721 ccatgccatc catgatccat tttttgcggc ctggctacct caatccggac agggttgctg
      781 aaagccgtcc caaccttctc taaggcaaca ccgttcgcca aatgtgcggc caccggcgaa
      841 aagatatcgc gaccgtgaaa ggtggagctg atattctcca accaatattc ttttcgatct
      901 aattcaacaa aatcggtacg ccaaccagct tgctctgcct gctcgagcaa gcccgtgatg
      961 gtgccattat caggacccac ataataccaa tcgccaatct gagccgccat cggacgacga
     1021 gccgtaccca cacccgggtc aacgacaacg acgtgaacag atccctttgg aaagtatgaa
     1081 atggaacgcg ccaatataaa ggcggcttct cgaatattct gggcttgaat caaatgactc
     1141 aaatctgaaa tctgtgcagt caggcaaatc ccccagatca cccctttcat cacgccgaca
     1201 gtgccatctt tgataccgaa atctgtcatt agggtgataa ccgacaacgc attctcctca
     1261 gcgattaaga atgcgcctat tatatagcat tcagagggta ttgccttgca tcaacgactt
     1321 tggctgcctt cggccaagag tctttgtttc ctgagaataa atagaggggg taacttaagc
     1381 tgaggcgcac ggtcactgga ccagggtgca tgtggcgcca tgacgaggcc acacagcagg
     1441 atgagtgcac ggtcgtaatt cacgcgccag ccagcgaagt ctcgccaggc ctggtctcgg
     1501 tcttccttga taggcaagcc tgcaatcgca agttggttga gaacatcttc gaattcctca
     1561 cgttccacac aaatatcatg ttccggatag tgagggtcct gcgggttagg gatatcgaaa
     1621 tacgtcgcga tccgccttaa cgacagatat ccagctcgaa tgcaaagcgc agcagacgcc
     1681 tcatagggga tctcgactgc cgaaagtgtg atggcagctg catccagaac ggttccggcg
     1741 gcggttaccc aggagttctc gggccgaggc gaacgaaaga agatcaaggc aggcagggtg
     1801 gtatggcttt cctccacatc ggcgaaccag gcttcccacg ttttccagta atcggacagt
     1861 ttctcgaggc catgaatgcg attgaaacgc aatagcatct cgagagcaga tggcggactg
     1921 ccggcgcgca cttcaagcat attaacaacc tgttctcgcc gcgcgaatgc agaatacatt
     1981 gttgggagat acgcgatcaa caaacccacc agcatcagac caatcatcgc ctcgctaaat
     2041 gcaagcaaag tcaccagcgg agtcttggag atttcaaacc cgagcgtgaa gagggacgag
     2101 ccgcttaatc gaaaaacatc aaataaatcc ccaaaaccta cagcccaata gatcgcggcg
     2161 tatccaacca agatgagtac ataccaggtc gggagcaaca gcatcaagcc gatgggagca
     2221 taatacgcca taattgcgtc ccgacggata tatgatttgg caaaatgagc aaagaaatcg
     2281 aagatacgcc gcaaaagtcc aaacacaaga cggttgagct gactgcgcgc cgcgcgcggt
     2341 aacacgaacg ttgatagcgc ggaggaaagc gtaaggaaaa ctacaacgat tccggcgatc
     2401 aagacgaggg tgggaaagaa ggattccatc atccaaatta taactctgcc ctcgcaccca
     2461 cgagcaaaat gcaaaactcg atatggtaat atttcgatga accagctact tcatcatgcg
     2521 gtacttacaa gtttcgaaat catggtgcag ctacaaactt acacaactat agattttcct
     2581 gcagctctaa agtgccagac gctttctttc ctgcgcattc agtggcccgg ggggttcata
     2641 ggcgagaaca ggctccgcga ttgggttacg aaagagtcag atcatcccat ccattttgtg
     2701 cttgtagaag cgggcatcct gatcagtcat acgaatgttg tttggaaata tctcgagcat
     2761 gaagggatgt cttataaagc atacggtctg acaggcgtat tcacatatcc agcattccgc
     2821 ggggaggggt acggaagcca gattattgag gcaggcacca actatatcaa gcaaagtgac
     2881 gccgatatcg ccatgctata ctgtgatgat agcctccata acttctatgc gcaacatggc
     2941 gggattccca tggatgattc gatcagttat attgactccg aggcaggacc ggttcttgtt
     3001 gaggatgaga tcttaatgat gcaattcatc tctccgaaag gacagcaaaa caggatttct
     3061 tttgaacgcc agcccattta tttcggaagt gatagcacgt ggtaatacga taaaatgatg
     3121 agtcaaaaac aattggagcg tgtacgaaac atctgcaccc agctccctga aaccactgag
     3181 agactttcac acggcgagcc caccttcttt gtgaagaaca aagtatatgt aatgttcgcg
     3241 aataatcatc ataacgatgg tcatatcgcg gtgtggctgc cggtgccgtc cggtttccag
     3301 tccaccttga ttgaagctgc gccccagaca tatttcatgc ctccttacgt gggggttcgc
     3361 ggctgggtgg ggattgagct cgctcaaatc agtgatcccg acctgacgta tcacattcag
     3421 gtggcctggg aattgaccgc cccgaaacgc ttattatcaa aagtcggaca actcaatgca
     3481 tctcaaccag accgtaaagg agaaatccac aaatgagttt ttctaaactt acaggtctct
     3541 cactcattgt attacagctc actttcaacg cagtcttttt tctcttacaa aggacttttg
     3601 aataccccga tattcttcgc aaaccgaccg actatatttt gagtcgattt aatcagggtg
     3661 gccggcaact gattatgaca tggtatgctt tcgccttcac cggagtgctt ttcgtgccgg
     3721 tggctttcct tgttcaccag acctttgcca gtgaaaatac tccgttcatg gcatttgcca
     3781 caacgattgg tctgttggca ggtgttagtg agtgatccaa atgactaaag caatcgacat
     3841 ttatttggag attggcagga aacgaacttt tgccggtgcc atagactggc ctggctggtg
     3901 tcgcagtgga gcaaatactg agtcagcact gcaggcgctg gtagagtatg gtccaaggta
     3961 tgcgcgcgtt cttcgattgg aaaagatcga attcaaacct cctgctgacg tatctgtttt
     4021 ttccatcgtt gaaaaactgg aaggcaacgc caccaccgat ttcggcgcac ccgggctggc
     4081 tccatccgcc gattcagagc tggtcaatga agaggaactt caacgcttcg aggcaatttt
     4141 gagagcctgc tggaaggcat tcgactcagc ggtgaaatct gcgcaaggca aagaactgct
     4201 caagggaccg cgcggcggtg gccgccagct cgatggagtc attaaacacg tcctcgaggc
     4261 cgatgagggc tatctttcca gcttgggttg gaagatccac aagaatgata agttcgatca
     4321 aagtgaggca ttaaagttca ggcgaaaaga gatcctggaa gggctagcgg cctctgcaca
     4381 tggtgaaatt ccaccccgcg gacctcgcgg cggacttcgt tggacaccac gttattatgt
     4441 tcgacgcgtc gcctggcatg tgatcgatca tgtctgggaa atcgaggata gaatcaatta
     4501 aggagaaact atggaagatg aagtattaat tcgacgagga aataccctgg tgcggcgtct
     4561 acagcttgaa gctggagaag cgatgccctg gcaccgtgac ccgttccatc gggtgacagt
     4621 tattttgcag ggcagtgcta ttgccataga atttcgcgat ggaagtaaag aagagcgatt
     4681 cacggttagg gctggccagg tagattggga tgaaccgacg gatcggcttc accgcggcgt
     4741 caatgtgggc gatgagccct acgaggaaat tacggtcttc ttcctcaaac actcggatga
     4801 cgtccctcaa cctgttgaag aataatgcgt tatagagctg agaggtctta aatttaacga
     4861 atacatgaga tcagaataga gtaaaggaga catcatgctg atcaatcccg aattggtgaa
     4921 tgccatcaac gcacagatcg gtcgtgagtt cggtgccagc atgcagtatc tgagcatcgc
     4981 cgggtatttt catgcccaaa aactaacgct gatagcaaaa ttgttttttg aacaggcaga
     5041 agaagaaaag atgcacgcta tgaagtttgt gcattatgtt cttgatacac agggcgagct
     5101 gcaaatccca atgatcgcgg cacctacagc gagtttcgca aatgctgagg aagccgtgca
     5161 ggcggcactg acctgggaaa cagaagtaac tgggcagatc aatcagctta tggatctggc
     5221 ggtttcacaa aacgattatc tggcacagaa cttcctgcag tggtttgtag atgaacaact
     5281 ggaagaagtg acaaaaatgg atcaactgct cagcgtggtc cgccgcgccg gggagcgaaa
     5341 tctgctgatg gtagaggcat acttaattca cattgataaa gctgggtaag cgagtccgca
     5401 tcggattcgc tttactttga tgttatgaac cggcagaacc ttgccggttc tttgttttat
     5461 ttcgcaatgg ctggttttgg aaacttttga cctgcggatt gcgttataac tccacattaa
     5521 atgtacagta ctaaaccctg taagattatc caactgaatt cttttcagtg cttttccaaa
     5581 aagtgaaggt aaagaaatgc gatttacacc atggttcaaa caattctttc ttgtgttctg
     5641 cctcttgtca atttcattag cggcatgcat cccaacgtca ggaacggcgg tttcgacatc
     5701 caccccatcg cctgcgccga gtctcccgct cacgggcgag cccgccgcta cggccgcgcc
     5761 gatcatcggg tccggacttc gagttgcttt tgtaaaggaa agaaatatcc atttgtggga
     5821 tgaatccacc gggcagagcc agattatttt caatgccggg gatgtgattg ccgtaaagat
     5881 gagtgaggat gggcaactga tcgcattcct tcgccgttca gcagtgcaac tgaccgaagc
     5941 agaatggcgc gagcaatcct ccctttggat agtggactcg aatggagaca atcctcgaga
     6001 actagtttcc gcagatgctt tacgtcaacg gctcaacgcg gtggaaagag attccacaaa
     6061 catccctcag ctggaatggg tgccaggaac acataaatta ctttttagtg gatggaccta
     6121 tttcgtcatg gcagaaggtg aatcgcatgc cgttccacag ggactttttc taatcgatgc
     6181 cgactcgctc accgatgcgg agctggttcc agctggtaat aatttgcggt ttgtgccatc
     6241 cctagatgga aaccagatcg cgttgatgtc tctcaatgga cttagcttca tcaataccga
     6301 tggaggcaat ctgcgcccga acgttttaac ctacacgcaa gtagggcgga ctgccccgct
     6361 tttcccaacg ggcgtttgga ctcaggactc cacagctttc ctggttaccg gctctctcga
     6421 agctgatccc gaatttaaca ttaacttcac catctggcgc gtaccggtgg atggctctgc
     6481 gcagacctcg ttagctacaa tcacaaaaag tgatccgcgc tcggttatgt tttctccaga
     6541 cggtcagcgt atcgcattca ttcaacccac ggacacgaat ccgccgttta gcgcgtcctg
     6601 gttcatcatg gcactaattg ggaatgcgag cccaatcgct attcagccga gtttcgaggt
     6661 ctggtctgcc tccctgcact ggtcgccagc cggtgaccct tttactggaa ccctcaaaaa
     6721 gctctgtccg gatgcctcaa acgatggcaa tatctgtgac gatcgcctct actttgacgg
     6781 ggcgctttcc gctattcatt ggatcgacag caaccgcgtc ctgtttctga ctcgcaaccc
     6841 ttccgtcttg ttcctcgcca ccatggattt cacaagtccc tgggatgcca cgacgattcc
     6901 cattgtcgcc tggccgctaa atgaatcggt aagcctgaac agctttacaa cagcaaagta
     6961 attcgctgaa agaggaccct cag
//
LOCUS       DASTJP010000798         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_599498, whole genome shotgun sequence.
ACCESSION   DASTJP010000798 DASTJP010000000
VERSION     DASTJP010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599498"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1053
                     /locus_tag="VFQ23_23365"
     CDS             199..1053
                     /locus_tag="VFQ23_23365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9909606.1"
                     /translation="METRRFGRTGHMSTVAIFGAAALWKISQEDADKVMEMVIEAGVN
                     HIDVAPSYGQAEERIGPWMPRERGRFFLGCKTTERTREGAWNEMQASLKRLQTESFDL
                     YQCHAVTTMEELDAVTMKGGALEAFVKARDEGLTKYLGITGHGVDAPQIYLEALRRFD
                     FDSVLFPINFVQMANPEYRKYTEELIATCNAKDVGTMIIKSITKAPWGERQHTASTWY
                     EPFDQMEEIQQAVNFVLSFDVTGVCTAGDTRILPMVLKACENFARLSNSEMEEMIKSG
                     KQFEPLFS"
     gene            1083..1763
                     /locus_tag="VFQ23_23370"
     CDS             1083..1763
                     /locus_tag="VFQ23_23370"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9909607.1"
                     /translation="MMSREFFDAIKEGNRDEVEHRLNLDPGLIHAKENGLSPIMVAAY
                     HQEPAIASFLADKTVAINIFEASATGKINNVIRLLARDPQLVNAYAEDGFQPLGLACF
                     FGHLETAEYLIKAGAPINFASRNGLKAAPIQSATAARHEKIVQMLLRLGADPNVREGN
                     GYTPLHAAAQNKDIDMIHTLIYGGADLTLAGKDGKTPLDLAVETGDQETIGLLAEGIT
                     KRLKPKRA"
BASE COUNT          614 a          545 c          576 g          483 t
ORIGIN      
        1 ggcatcgtca aaggacatcg cgcgaccggc tcccttggta ttaaagatga cctggtcaac
       61 gggggcgcga cctgggtaga tgaacccgcg tttcgagacg cgaaccaggt ctggggtcgg
      121 gtggttgagg atatcccagc cttttgcaga gaactggtaa aactcatcgc tgaataacaa
      181 attacgaagg agtcaaaaat ggaaacaaga cgattcggac gaacaggtca tatgagcacg
      241 gttgccattt tcggtgcggc cgcgctttgg aagatctcac aagaagatgc cgataaagtg
      301 atggaaatgg ttatcgaggc gggcgtgaat catattgatg tcgcgccatc ctacggtcag
      361 gctgaagagc gtatcgggcc atggatgccg cgtgagcgag gcagattctt cctcggatgc
      421 aaaaccacgg aacgcacgag agaaggtgcc tggaatgaaa tgcaggcctc acttaaacga
      481 ttgcagaccg agtcatttga tttgtaccaa tgtcacgcgg tcacaacgat ggaggaactg
      541 gatgcggtga ccatgaaggg cggcgcgttg gaagcctttg tgaaagcgcg tgacgagggc
      601 ttaacgaaat atctaggcat taccggtcac ggcgtggacg caccgcaaat ctatctcgaa
      661 gccttgcgac ggtttgattt cgactccgtc ctgttcccta ttaacttcgt tcaaatggca
      721 aacccggagt atcgaaaata tacagaggaa ttgatcgcca cctgcaacgc caaagatgtg
      781 ggaacaatga tcatcaagtc gatcactaaa gctccctggg gcgagcggca acatacggct
      841 tcaacctggt acgagccatt cgatcagatg gaggaaatcc agcaggcagt aaacttcgtg
      901 ctttcctttg atgtcacggg cgtctgcaca gcgggtgaca ctcgcattct tcccatggta
      961 ttgaaagcct gcgaaaactt tgctcgcttg agtaattctg aaatggaaga aatgataaaa
     1021 tcaggaaagc aattcgaacc attgttttcg taacaacgtt cgcggtcaat cagggcaggt
     1081 gcatgatgag cagggaattt tttgacgcaa tcaaagaggg aaacagggat gaagtggaac
     1141 accgcctcaa tctggacccg ggcctgatcc acgccaaaga aaatggactc agccctatca
     1201 tggttgccgc ctatcatcag gagccggcga tcgcttcctt cctggcggat aagaccgttg
     1261 ccatcaacat cttcgaagcc tcagcgaccg gcaaaatcaa taatgtgatc cgtttgctgg
     1321 cgcgagatcc ccaactcgta aacgcatatg ctgaagacgg attccagccg ttaggactgg
     1381 cctgcttttt cggtcatttg gaaacggcgg aatacctgat caaggcgggc gcgccgataa
     1441 acttcgcctc ccgcaatggt ctcaaagccg cgccgattca atccgccaca gccgctcgtc
     1501 atgaaaagat tgtacaaatg ctcctccggc ttggagcaga cccaaacgtc cgcgaaggaa
     1561 atggctacac tccactccac gccgcagcac agaacaaaga tatagacatg atccacacct
     1621 tgatctacgg tggcgcagat ctgactctgg ccggcaaaga cggaaaaacc ccgctcgatc
     1681 ttgctgtgga aactggggat caggaaacta ttggattgct tgcagaggga attacaaagc
     1741 gactgaagcc gaaaagggcg taacgatcta aagaccaaca gcagagcctc ctaaaccata
     1801 actacccgtt agtttctcgt tttcccactc gctgcagatt gaagagctca accctgcttg
     1861 cttgatctaa tggtcacaaa gaccattcca tgaacaacgg actgatccag agcctcagtg
     1921 atgaaggcaa caagctcctg gctgcgttgc gagtaatctg ccagacaacg ggaaaacgtt
     1981 acagtccaaa ttaccccctg tatccaattc atatcttact actgtaacca aaatgttact
     2041 cgtgcggtct tgtatttagt ggttttatgc ctaaaatgaa tattattatt gttcccgagc
     2101 ttgcggcgaa ggtggggtcc atgcagccag gggaggatgc ctcttatgag gcatcaataa
     2161 tttgtatcat aaatgtcaca cgcaaacact tgaacggaga atgaggagat ctgtagaa
//
LOCUS       DASTJP010000799         7093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1340163, whole genome shotgun sequence.
ACCESSION   DASTJP010000799 DASTJP010000000
VERSION     DASTJP010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340163"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFQ23_23375"
     CDS             <1..939
                     /locus_tag="VFQ23_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909608.1"
                     /translation="QIMTSTAAPLQLEGLKTPVSRTPLQNALRRFIRPRSARVGMTIL
                     GLLVFVAIFASVLAPHDPIKPLRDAKRRSPPCIHLLGCPADQPQHFFGIDSNQRDLLS
                     RIIYGSRLSLEIGLATVTFAIIIGMALGALGGFAGGWTDNIIMRFMDVLLAFPSLLLS
                     IAIVAVLGPGLINALIAIAFVSIPVYARIVRASVLQVKEQDFVSASRALGASPFGILT
                     GHVLPNAITPLIVQATLGIASAILDAAALSFLGLGAAPPTPEWGLMLGEERNSVFNAP
                     HLVFIPGIAIMLTVLAFNLIGDGLRDALDPRLVNVS"
     gene            1240..3075
                     /locus_tag="VFQ23_23380"
     CDS             1240..3075
                     /locus_tag="VFQ23_23380"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9909609.1"
                     /translation="MKRNNLFVLLSILVLASMVLSACGGAATPTEAPVATEPPATEPP
                     AATDAPATEAPAATEAPAACTEPAGTEPIAFPDGGKSVTGAWDQEPDSIVPYFSQMSF
                     AVWVTQFTLAGLGEWDDQNNFVPELAAEVPTAENGGVSEDGLTITWKLKDCLYWSDGT
                     PITSDDVKFTWEAVMDPGNAVATRTGYDKIASIDTPDPLTAVITFSELYPPWQTLFTQ
                     GPNNSGAILPKHMLEGETALESNDFIHQPTVGSGPFVITEWVPGDFMTLLPNPNFHAG
                     RATLDRIQIRFVPDSATALAALQTGDIDWYPNFSEADIATVSALEPDIHLIVTPGANF
                     EHYFFNLGRTEGVDGRGAADNEGFCPFKDINVRKAITLGINRQAFVDALLEGKTTVPV
                     SQWPNSTWTNTDLVVDQYDPEAAAALLDEAGYTVGSDGIRVGDCNGEQTRLSFNFETT
                     TAPIRIDIATAVQADLAAIGVEFTPIHTPAGTFFGQYVDGGPLTTGNYDMGGYTTGFY
                     PDPYTDNYNCENVPSAEKPDGTNWYHNCDPFLSDLMEEVNASADPAVRKATLDEAQKY
                     IFDNYYVIMMYARANVQAHVDRMVLGPFGYYSNMNWNAEVWDVKE"
     gene            3205..4164
                     /locus_tag="VFQ23_23385"
     CDS             3205..4164
                     /locus_tag="VFQ23_23385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909610.1"
                     /translation="MWSYIARRVIQASFTLLIITIICFLLTRFSGDPMAQYAANPRMT
                     AEDRAALRERLGLNQPLPVQYLKWLGLALQGDLGKSFFSKQPVTEMIATRLPNTLVLM
                     FTAEIITVGFALLLGILAAVKQYSAADNIITSFSFVGFSMPIFFIALGLMLIFAVQFK
                     EWGLPYLPTGADVWDKRDPVELATHLVLPVTALAIVNTAGYSRFVRTSMLEVLGLDYV
                     RTARAKGLTSRVVLMKHALRNAALPLVTIVGLDIPALLGGALVTETVYAWPGMGRLFW
                     EYAQRGDYPVVLGVLLITSSAVVVFTIITDVVYTFVDPRIRLS"
     gene            4174..5130
                     /locus_tag="VFQ23_23390"
     CDS             4174..5130
                     /locus_tag="VFQ23_23390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909611.1"
                     /translation="MATGTTLDIPVLGEREASRPILTPRQLIWRRFRKHRMALLGMIG
                     FLLLLAFIIVGSILISEKEANEVDLQARLSPPSAAHWMGTDSTGRDLFARIIHGGQIS
                     LIVGVLAVILSVTVGTLIGGIAAYYGGWVDSLLMRFTEAMLSIPSLFLLIILGKFFGK
                     DVQTIEVLGRTFSGSVGIVILVIGLTSWMYLARIVRANVLSLRELDYISVSKSLGATD
                     MRIFFNHLIPNTMGAIIVSSTLGLAGAILSEAYVSFLGLGVQPPTASWGNILTSAQSL
                     IQRGSWWVWLFPGTFIIITILSINLVGDGLRDAFDPRSTRHL"
     gene            5431..>7093
                     /locus_tag="VFQ23_23395"
     CDS             5431..>7093
                     /locus_tag="VFQ23_23395"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9909612.1"
                     /translation="MKRSLLLLLSMLVLASMVLSACGGAATATEAPVATEPPSTEPPA
                     ATEPSAATEPPAAACEPVGTEPIAFPDGGKSVTGAWDQEPDSIVPYFSQMSFATWVTQ
                     LTLVGLAEWDDQSQFVPELAAEIPTTENGGVSEDGLTITWKLKDCLFWSDGTPITSED
                     VKFTWEAVMDPGNAPATRTGYDLISSVDTPDPLTVVLNFSELYPPWQTLFTQGPNNQG
                     AILPKHLLEGETALESNDFIHWPTVGSGPFIITEWVPGDFMSLLPNPNFYKGRPILDR
                     VQIRFVPDSATALAALQTGDVDWYPNFSEADISTIDALEPAVHLLVVPGSDFEHYFFN
                     MGRVEGVDGRGPADNEGFCPFKDINVRHAITIGINRQAIVDALLEGKTTVPVSQWPNT
                     AWTNTDMQVEAYDPDAAAALLDEAGYTVGSDGIRVGDCNGEQTRLAFNFETTNSPIRI
                     DIATAVQADLAAVGIEFTPIHTPAGTFFGQYVDGGPLTTGNYDMGGYTTGFYPDPYTD
                     NFNCENVPNAEKPDGTNWYHNCDPFLSDLIDSSLQTVDTAERKRII"
BASE COUNT         1594 a         1961 c         1756 g         1782 t
ORIGIN      
        1 cagattatga catcaactgc cgctccgctc caactggagg gactcaaaac tcctgtctcg
       61 cgcacacctc tgcaaaatgc gctgcgacgt ttcattcgcc ctcgttcggc gcgcgttgga
      121 atgaccattc tagggctgct cgtctttgta gcaatctttg catcggtact ggcaccacat
      181 gacccgataa aaccactccg cgatgcaaag cgtcgttctc cgccttgtat tcatctcctg
      241 gggtgcccgg ccgaccagcc acaacatttc tttggtattg atagcaatca acgcgatttg
      301 ctttcccgca tcatctatgg ttcgcgtctc tctttggaga tcgggcttgc gacggtgacg
      361 tttgccatca ttatcggcat ggcattgggt gcattgggtg gcttcgcagg cgggtggacc
      421 gataacatta ttatgcggtt catggatgtc ttacttgcgt ttccaagttt gttattatcc
      481 attgcaattg tggcggtgtt gggtcccggc ttgatcaatg ctttaatcgc gattgcattt
      541 gtttcaatcc cggtctatgc acggattgtg cgtgccagtg tccttcaagt gaaggagcag
      601 gattttgtat ctgcttcgcg tgcactcggc gcttcaccgt ttgggattct gaccggtcac
      661 gttctaccga atgcgattac gcctttgatc gtgcaggcaa ccctggggat cgcatctgcc
      721 attcttgatg ccgcagcact ttcttttctt ggactgggcg cggcaccacc aacaccggag
      781 tgggggctga tgcttggtga ggaacgcaat agcgtgttca acgctccaca tcttgtcttc
      841 atccccggta tcgcgatcat gctcactgta ctggcattta acctgatcgg cgatgggcta
      901 cgagatgcac tcgacccaag attagtcaat gtaagctaga aagcagccac aagctaaaag
      961 caaatgaggg tgggcacaaa gaattcaaaa tccaataaca aattaatcat atcctcttgc
     1021 gtaaatcagt gtaatgaatc acctcgcaag agggtatttt tctaaatata tccccctctg
     1081 tagtggcgtt aacggtactg gtatacgcac tacccatgta ctattttatg atttgctgta
     1141 taatcatttc actcattcga aaggaggtgc tttgccacaa atatttgttt gaggttccct
     1201 acgtcgtaac taaaccataa attctctttg gaggagaacg tgaaacgcaa caatctattt
     1261 gtattactct caatactcgt gctggcgagc atggtgttgt cggcctgtgg tggcgctgcc
     1321 actccgactg aagcaccggt agccactgag ccacctgcca ccgaaccgcc tgctgctaca
     1381 gatgcccctg caacagaagc acctgccgcc actgaagcac cggctgcctg taccgaacca
     1441 gcgggcactg aacccattgc tttccccgat ggtggcaaat ctgtcaccgg cgcctgggat
     1501 caggaacccg attccatcgt tccgtatttc agccagatgt cctttgcagt ttgggtaacc
     1561 cagttcaccc tcgccggatt gggtgaatgg gatgatcaaa ataatttcgt gcccgaactt
     1621 gcagccgagg ttccgacggc cgaaaatggt ggggtatctg aagacggctt gaccatcacc
     1681 tggaaactga aggattgtct ctactggtca gatggcacac ccattacttc agacgatgtg
     1741 aaattcacct gggaagctgt aatggatcca ggcaatgccg ttgcgacccg cacagggtat
     1801 gacaaaattg ccagcatcga tacacccgat cctttgaccg cggtgatcac cttctcagaa
     1861 ctgtatccgc cctggcagac tctgtttacc cagggaccaa acaacagcgg cgcgatcctt
     1921 ccaaagcata tgcttgaagg cgagactgca ctggaaagta acgacttcat tcaccagccg
     1981 acggttggtt caggaccatt cgttatcacg gaatgggtgc ccggcgactt catgacgctg
     2041 ctccccaacc cgaacttcca tgccggacgc gcaacgcttg accgaatcca gatcagattc
     2101 gtgcccgatt ccgcaactgc gctggctgcg ttgcagaccg gtgatattga ctggtacccg
     2161 aacttctcag aagcagatat tgccacagtg agtgccctgg aaccggatat tcatttgatc
     2221 gtaactcctg gcgctaactt tgagcattac ttcttcaacc tgggtcgcac ggaaggtgtg
     2281 gatggacgcg gcgcagccga taacgaaggc ttctgcccgt tcaaagacat caacgttcgt
     2341 aaggctatca ctctgggcat caaccgccag gcattcgttg atgcactgct cgaaggcaag
     2401 accacggttc ccgtaagcca gtggccgaac tcaacctgga cgaatactga tcttgttgtt
     2461 gatcagtatg atccggaagc tgcagccgcc ttgctggatg aagcaggcta caccgttggc
     2521 tcagatggca tccgcgtggg tgactgcaat ggtgaacaga cccggctctc cttcaacttc
     2581 gagaccacaa ccgctccgat ccgtatcgac attgctaccg cagtccaggc tgacctggcc
     2641 gcgatcggtg ttgaattcac tccgatccac acccccgccg gaaccttctt cggtcagtat
     2701 gtggatggtg gcccattgac cactggtaac tatgacatgg gtggctacac cactggtttc
     2761 tacccggatc cttataccga caactacaat tgtgaaaatg taccgagtgc tgagaagcca
     2821 gatggcacca actggtacca taactgtgat cccttcctct ccgatttgat ggaagaggtc
     2881 aatgcctcag cagatcctgc ggttcgcaaa gccacgctgg atgaggccca gaaatatatc
     2941 tttgataact actatgtcat catgatgtat gctcgtgcca acgttcaggc gcatgtcgat
     3001 cgcatggtgc tcggaccctt cggctactac agcaatatga actggaatgc tgaagtctgg
     3061 gatgtaaaag agtaagtgaa ttagcaagtc agaaatagat gttcaaaatg agaggcaggg
     3121 aagcccctgc ctctcattca aataaaaaag tatttaaccg aaagatatgc cttatcagtt
     3181 ataacgtgaa taacggagac tcccatgtgg tcctatatcg cgcgaagagt aattcaggca
     3241 tcttttaccc ttttgattat cactattatt tgtttcctcc tgacgcgttt ttcaggggac
     3301 cccatggctc agtatgccgc taacccgcgc atgaccgctg aggatcgagc cgccctgcgc
     3361 gaacgtttgg gactgaatca gcctctcccc gtccagtatt tgaagtggct gggtctggca
     3421 ttacaagggg acctcggaaa atccttcttt tccaagcagc ccgtcaccga gatgatcgcc
     3481 actcgcctgc ccaacacctt agttctgatg tttaccgctg aaattattac tgttggcttt
     3541 gctctccttt tgggtatttt ggcggccgtc aagcaatact cagcggccga taacatcatc
     3601 acttcatttt ccttcgttgg cttttcgatg ccgatctttt tcattgccct gggactgatg
     3661 ttgatctttg ccgtgcaatt caaggaatgg ggcttgcctt atcttccaac gggagcagac
     3721 gtctgggaca agcgcgatcc ggtcgaactg gcgacgcacc tggtactgcc ggtaacggcc
     3781 ctggcgattg tcaatacagc tgggtattct cgttttgtgc gcaccagcat gctggaagta
     3841 ttggggctcg attacgttcg caccgcccgt gccaaagggc tcaccagtcg cgtagttctg
     3901 atgaagcacg ctctacgaaa tgccgcctta cccctcgtga ccattgtcgg tctggatatt
     3961 cctgcattgc tcgggggcgc gttggtgact gaaacggtct atgcctggcc ggggatggga
     4021 cgtctgttct gggagtatgc acaacgcggc gattacccgg tggtgctcgg tgtgttgtta
     4081 atcacctcat ctgcagtagt tgtgtttacc attattactg atgttgttta cacctttgta
     4141 gatcctcgca ttcggttgag ttaggaggaa agaatggcaa caggaacaac cttggatatt
     4201 cccgtcctcg gggaacgtga agcttcacga ccgatcttga cccctcgtca gttgatctgg
     4261 cgacgcttcc gcaaacaccg catggctctc ctcggtatga tcggctttct cctcctgctc
     4321 gcattcatca tcgttgggtc cattctcatc agcgagaaag aagctaacga agtcgatctg
     4381 caggcacggc tcagcccgcc atccgctgcg cattggatgg gcaccgatag caccggtcgc
     4441 gatctgttcg ctcgcatcat tcacgggggt cagatctccc tgatcgtcgg tgtgttggca
     4501 gtcatcctct ctgttaccgt gggaaccctg atcggtggca tcgccgccta ctatggtggc
     4561 tgggtcgatt cactcttgat gcgtttcacc gaagcgatgc tctccatccc ttcccttttt
     4621 ctgttgatca tcctcggcaa gttctttggc aaggatgtgc agactatcga ggtcctgggt
     4681 cgcaccttca gtggcagcgt gggcatcgtc atcctcgtga tcggtctcac ctcctggatg
     4741 tatctcgctc gcatcgtgcg cgccaatgtg ctctccctca gagagttgga ttacatctcc
     4801 gtgtccaaat cgttgggagc cactgacatg cgcatcttct tcaaccacct gatccccaat
     4861 accatgggtg ccattatcgt ctcctccacc cttggattgg ccggagcgat tctttctgaa
     4921 gcctatgttt cgtttttggg attgggtgtt caaccaccga ccgcatcctg gggaaacatc
     4981 ctgaccagcg cacagtcgtt gatccagcgt gggtcgtggt gggtctggtt gtttccgggc
     5041 accttcatca tcatcaccat cttgtccatc aacctggtcg gcgatggtct cagagatgcc
     5101 ttcgacccca gaagcaccag acatttataa aatataaaac caggcaacgg gcgcctacag
     5161 cgcccgtttt ttgtttaact cttaagacaa gcacgaatgc tataaatcct gaatcaatag
     5221 ccgttcttat ccaataaaca gggactgaaa aatacgcacc agacaagcgg ggaatgacgc
     5281 ctctatggag gggtctgtat ttgtaatcgc taccctataa ttttataagc aggtaatgaa
     5341 agacgatatg gtatggaagg aggcccatcg tcacttatat ttttatttaa ccgcattcca
     5401 aactaacaaa aattctcttt ggaggagaac gtgaaacgca gcctactttt attactttca
     5461 atgcttgttt tggcgagtat ggtgctctcc gcctgcggtg gtgctgccac tgctactgaa
     5521 gcaccggtag ccactgagcc accttcaacc gaaccacccg ctgctacaga accatctgcc
     5581 gcaaccgaac ccccagcagc cgcttgcgaa ccagtgggca ctgaacccat tgccttcccg
     5641 gatggcggca agtctgtcac cggcgcgtgg gatcaggaac ccgattccat cgttccgtat
     5701 ttcagccaga tgtcctttgc tacctgggtg actcaactga cgctggttgg tctggcagag
     5761 tgggatgacc agagccaatt cgtgccggaa ctggcagcag aaatcccaac cactgaaaac
     5821 ggtggtgtat ctgaagacgg attgaccatc acctggaaac tcaaggattg tctgttctgg
     5881 tcagatggca cacccattac ctcagaagat gtgaagttca cctgggaagc tgtgatggac
     5941 cccggtaatg caccggcaac gcgcactggc tatgatctga tttccagcgt tgacacaccg
     6001 gatccactga ccgtcgtgct caacttctca gaactatatc cgccctggca gactctgttc
     6061 acccagggac ccaacaatca aggcgccatt ttgccgaaac acctgttgga aggtgagacc
     6121 gctctggaaa gtaatgactt tatccattgg cctactgtag ggtccggacc tttcatcatc
     6181 acggagtggg tacccggcga ttttatgagc ctgctgccta accctaattt ttacaagggt
     6241 cgccccatac tggatcgagt ccagatccga ttcgtgcccg attccgccac tgcgctcgcc
     6301 gcacttcaga cgggcgatgt ggattggtat ccgaacttct cagaagcaga tatttccaca
     6361 atcgatgcac tcgaaccagc tgtccacctt ctggttgtgc ccggttcaga ctttgagcat
     6421 tacttcttca acatgggtcg cgtcgaaggt gtggacggac gaggtccagc cgataacgaa
     6481 ggcttctgcc cattcaagga tattaatgta cgccatgcca tcaccattgg tattaaccgc
     6541 caggccattg tagacgcctt gctcgaaggc aagaccacgg ttcccgtgag ccagtggcca
     6601 aatacggcat ggacaaatac cgatatgcag gttgaagcct acgacccgga cgccgccgca
     6661 gccttactgg atgaagcagg ctacaccgtt ggttccgatg gcattcgcgt gggcgactgc
     6721 aatggtgaac agacccgcct cgccttcaac ttcgagacaa ccaactcccc gatccgtatc
     6781 gacatcgcca ccgcagtcca ggctgactta gctgccgtcg gcatcgagtt cactccgatc
     6841 cacacccccg ccggaacgtt cttcggtcag tatgtggatg gtggtccgtt gaccactggc
     6901 aactacgata tgggtggcta caccactggc ttctacccgg acccatacac agataacttc
     6961 aactgcgaga acgtgccgaa tgctgaaaag ccagatggca ccaactggta ccataactgt
     7021 gaccccttcc tctctgattt gattgactct tcacttcaga cagtcgatac agcagagcgc
     7081 aagagaatca ttg
//
LOCUS       DASTJP010000800         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1082141, whole genome shotgun sequence.
ACCESSION   DASTJP010000800 DASTJP010000000
VERSION     DASTJP010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082141"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VFQ23_23400"
     CDS             complement(<1..1349)
                     /locus_tag="VFQ23_23400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909613.1"
                     /translation="MSNPVRILIVEDRISDFELARREIARVVKNYELHQVETEEAYLE
                     ALETLQPDLILSDYSLPGFDGRTALTLALEHAPFTPLIIWTGTANEDTAVDIMKAGAT
                     NYVIKENIKRLGPAVLHALEERDLRLKRKRGDTEREALFEIMKGLADTEDLSAFLKII
                     HSSIARVIDAQNFMVIFYREDTMSYEAMYSVDQYDSPAVSAQLERSAAAYVCQCEEPV
                     LLTKTEYEALIDQGQIGSVGTRPACWLGAPLKTSHGVIGVIAVQNYENPNAYSEQDKE
                     FLAGISSQVAVAVERRQAEEVVRESQRFAESTLNALNSHIAVLDENGTIIAVNRAWRE
                     FSDLNGGDPTKTGPGVSYLAVCEAASALGTEGPLIAAAIRSVLRGDRKDYSLVYSCPS
                     AFERRWFWVRVTAFETGGPVRIVIEHQNITERKEAELELRKSRARYQEIFDHSPVSL"
     gene            complement(1423..1863)
                     /locus_tag="VFQ23_23405"
     CDS             complement(1423..1863)
                     /locus_tag="VFQ23_23405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909614.1"
                     /translation="MTHLNMIEILLVEDNPRDAELTLRALTKNNVANRVFHVEDGEEA
                     LDFLLCRGKYTGQETARSPRVVLLDLKLPKLGGLEVLRAIRADERTRTIPVVVITSSS
                     EDPDIANAYDLGANSYVVKPVNFDAFMNVVNSLSFYWLMVNHPR"
     gene            complement(1841..>2023)
                     /locus_tag="VFQ23_23410"
     CDS             complement(1841..>2023)
                     /locus_tag="VFQ23_23410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909615.1"
                     /translation="FQRLHTEREFEGTGVGLAIAQRVIHRHGGRIWAEGEQGKGAVFY
                     FALPKKEKEHDSSKHD"
BASE COUNT          468 a          575 c          476 g          504 t
ORIGIN      
        1 caaagtgaga cgggggaatg atcgaaaatc tcctgatacc gtgcccggct ttttcttaat
       61 tccaattcgg cttcttttcg ctccgtgatg ttttgatgct cgatcacgat acgcactggc
      121 cctccggttt cgaaggcagt aacacgaacc cagaaccatc ttcgttcaaa ggcagatggg
      181 cacgagtata cgagagagta atccttacga tcgccccgca agacggagcg aatggcagcg
      241 gcgatcaacg gaccctcggt tcccagggca gaggctgcct cacaaacagc gaggtagctc
      301 acacccggcc cagttttcgt ggggtcccca ccatttaagt ctgaaaattc acgccatgcg
      361 cgattaacag cgatgatcgt gccattctcg tcaagaactg cgatatgaga gttcagtgca
      421 ttcaacgtgg actcggcaaa tcgctgactc tcacgcacca cctcttcggc ctgcctgcgc
      481 tcaacggcca ccgcgacctg tgagctgatg cccgccagga actccttatc ctgttcagaa
      541 tatgcgttgg gattttcata gttctgtact gcgataacgc caatgacacc gtgggatgtt
      601 ttgagcggcg cgccgagcca gcacgccggc ctggttccaa ctgatccgat ctgaccctga
      661 tcgattaaag cttcatattc agtcttcgtc agaagcaccg gctcttcgca ttggcaaaca
      721 tatgcggcag cgcttcgttc caattgagcc gacacggcag gcgaatcata ctgatcgaca
      781 gaatacattg cctcatacga catggtatct tctcggtaga agatgaccat gaagttctgt
      841 gcatcaatca ctctggcgat cgaggaatga ataattttaa ggaaagcgct taaatcttcg
      901 gtatcggcaa gccctttcat gatttcaaac agcgcctcac gttctgtatc cccccgtttg
      961 cgtttcaatc gcaagtctct ttcttcgagc gcgtgcagga cggcaggtcc gagccttttg
     1021 atattctctt tgatgacgta gttggttgca ccggctttca tgatgtcgac cgctgtgtct
     1081 tcattcgccg tgcctgtcca gatgataagc ggcgtaaacg gcgcatgctc aagcgccagg
     1141 gtcaaggcag ttcgcccatc gaagcctggc aaagagtaat ctgaaagaat gagatccggt
     1201 tggagagtct ccaatgcctc caaataagct tcctccgttt ccacctggtg aagttcatag
     1261 tttttcacga ctcttgcgat ctcgcgtcgt gccaattcaa agtctgaaat ccggtcttca
     1321 acaatcagga tgcgcacagg attgctcatg tgtaaatttc cttctcaact cactccgccg
     1381 ggaattccag caaacatttc acagatcttt tctgcgcgtt tatcaccgag ggtgattgac
     1441 catcagccag taaaaactga gagaattaac aacgttcata aacgcatcga aattcactgg
     1501 tttaaccacg taactgttcg ctcccaaatc ataggcgttg gcaatgtccg ggtcttcgga
     1561 ggacgaagtt atcacgacta cgggaatcgt ccgcgtccgt tcatccgcac ggatggcacg
     1621 caacacctcc agtccgccga gcttgggcag cttcaaatcc agcaacacca ctctcgggga
     1681 acgcgctgtt tcctgtccgg tatattttcc cctgcacagc aggaaatcca aagcttcttc
     1741 accatcctct acatgaaaga ctcggtttgc cacgttattc ttggtcagtg cccggagggt
     1801 caactcggcg tcgcgtgggt tgtcctctac cagcagaatc tcaatcatgt ttagatgagt
     1861 catgctcttt ctcctttttc ggcagagcaa agtagaacac ggctccttta ccctgctcgc
     1921 cttccgccca gatccgccca ccgtgtcggt gtatcacgcg ctgggcaatc gccagcccca
     1981 cacctgtccc ttcaaattca cgttcggtgt gcaatcgttg aaa
//
LOCUS       DASTJP010000801         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1129311, whole genome shotgun sequence.
ACCESSION   DASTJP010000801 DASTJP010000000
VERSION     DASTJP010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129311"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFQ23_23415"
     CDS             <1..346
                     /locus_tag="VFQ23_23415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909616.1"
                     /translation="LIIVNSTEGALYTVDPNTGIATRIDLGTGAVPAGDGILLQGKTL
                     YVVQNQLNQIAVVKLSTDLSSGTIEETITSPNFRVPTTIAQFGNSLYAVNARFGTPPT
                     PDTEYEVVQVPR"
     gene            519..1199
                     /locus_tag="VFQ23_23420"
     CDS             519..1199
                     /locus_tag="VFQ23_23420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909617.1"
                     /translation="MAKASTLGKNTPVRDGTGTSIKDYTSLLGVVAAGILGMSLLCIT
                     SAIALSAPNQSKPIVLEAGNIVDLAVNNINTVSALTVQALSTISIDSTPILLPSRTPT
                     SSPTVTASPVLIPPTQSQPTRTRRPRIPPTFTPRPPPTRTPLPPPTNTAVPPSPTSIP
                     PTFTSIPPTFTSIPPTQTSVPPTVTPEPPTATPNPPTVPPDTSTPADPPPTVPVVPIV
                     DSSPTSGP"
     gene            complement(1281..1970)
                     /locus_tag="VFQ23_23425"
     CDS             complement(1281..1970)
                     /locus_tag="VFQ23_23425"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9909618.1"
                     /translation="MAIKLVVPVNGPITQLFGENPERYKQWGFPGHNGIDYGIANGTP
                     VGAAASGTVAAVSFENGGYGNYVKLSHMDGSKPYFTYYAHLASAAVSPGQKVKVGSMI
                     GFSNNTGASTGPHLHFGVKIDGQNPAYKGYVDPMPLFTVGDAADSDETTSTDQFTDAI
                     KLPNLSFEVLSEFLNVRNGPGVEFAIVGQLQKGAKVTGKRMQSKSVWIEFERGKWCAV
                     AFNGVVNLKVK"
     gene            complement(2032..2673)
                     /locus_tag="VFQ23_23430"
     CDS             complement(2032..2673)
                     /locus_tag="VFQ23_23430"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9909619.1"
                     /translation="MTPDTFQIRTCLSCGLRYPMTNESTFGMRCPVCLGETELSVRVN
                     TDLKTNLTSIRSTESRRRVSVLLDNIRSAWNVGSIFRSADGFGFAHVYLCGITPTPEN
                     EAVTKTSLGAENYVSWSYHKDAVQLVGALKKEGGKVWALEEHERAVHLSKHVDADSTN
                     PVILIVGNEVTGVDPGLIELSERIFFIPMRGEKTSFNVAIAFGIASYQLTGLS"
     gene            2716..>6247
                     /locus_tag="VFQ23_23435"
     CDS             2716..>6247
                     /locus_tag="VFQ23_23435"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9909620.1"
                     /translation="MHRVVPDLILENYRRGLYHGEFPTIALFLDLSGFSTMTDVLMQQ
                     GQHGAEVLAGLMHTVFDPLVDGIFAYGGKIISFAGDGILALFPIESSEKSSALTALAA
                     AWDIQQTLGENPEHETVYGKFRFSVKIGLAFGTVSWGILHSQDRQQATYYFRGTPVDV
                     SAAAEKHASAGEILITDELNELLKNEIEATPFGPFHKLHGFRVAMPVPTPTALDPIDL
                     EAARIFIPEKIIVQDVRGEFRQIVNLFMRFPDLPNEQLDQFFYIIFDLHKKYGGLLNR
                     LDFGDKGCNMLMLWGAPVAYENDIRRALNFILDLQSRVTFPITAGITYYVAHAGYLGS
                     VMCEDYTCYGWGVNLASRFMMSAPQGKIWVDDRIARRVATRFEIENVGEQRFKGFSAE
                     QKVNLLKGHKREAEAIYLGELVGRENELAALESFTDPLWNGNFAGMLLVLGDAGTGKG
                     RLVHDFRASEVFEKHYALWALCQADQLVRQSFNPFRSWLFRYFGFASANNVDDRKQIF
                     VSKLQALRAAIQDETLAQELDRLHFALGALLDLRWIDSPYEQMDAEGRHNNTILGLIA
                     LIKAESLRQPLILFIEDMQFIDHDTLQFLPRLKRTLLAGQESYPVGIIATSRVQPGNP
                     ITEGLIDSQIELGGLSLEAVARLIEIQLGGVPAVELVKLVMARSEGNPYFVEQIIRYL
                     QEEHVIEMSERGWNQVRRTRDFFVPSDLRVVLVARLDQLTQEVKDVVQTASVLGREFA
                     ISVLTEMTDEADNIERVVADAVQSEIWAPQDEVRYVFTHGLLRDAAYTMQMRARRQEL
                     HKWAVLALEKIYADELKFHYPELAYHAERANLHQKAQEYFLLAGQASAEVYSNSQAID
                     YFTQALTFTPADDVAGTFELLAERAALFHRVGDRIRQLRDIESLEQLAKEPTDLNGMA
                     RVEMLKAYYCFATGDYLSLIDHARRVMDLNIPMNAEMAMDTYSIWSLGLLRLGRLDAA
                     MEIAQDGLDLAQASRNIPKQGSILNSMGLIALEQKNPAVGRGYFELALSIAREVSDQR
                     LEAQCLNNLGNSAGFVQGDYSAAMQYYEQANVIFRERGDRSSEGMTLNNLGWAAGMLG
                     DFISARGHNERALLIAREVGNRYQETYTRINLSAVTGIDGDAQASLDYAHTAQELAIA
                     AGELSGEAWALLYKGHALLMLGDSERARENF"
BASE COUNT         1523 a         1553 c         1580 g         1591 t
ORIGIN      
        1 tctgatcatc gtcaacagca ctgagggtgc actttatacg gtggatccga acacgggtat
       61 cgctacgagg attgacctgg gtaccggagc tgtgcctgcc ggtgatggaa tcctgctgca
      121 aggtaaaacg ctttatgtgg tgcagaacca gctcaatcaa attgcagtgg taaagctaag
      181 tacggatctg agctctggta cgatcgagga gacgatcacg agcccgaact tccgcgtgcc
      241 gaccacgatc gcgcagtttg gaaatagcct gtatgcagtc aacgcacgct ttggcacgcc
      301 gcccacgccc gataccgaat atgaggtcgt gcaagtaccc agataacagc tgaagaccaa
      361 ccgattgttt gcatgccgcc ccgacaattg cgcaaaacgt ggatgtcggg gcggttttcc
      421 atcaggctgg aatttcaacc tgcaactgta actaattgga aagggtgaaa gaaaggagtt
      481 atgggataat tcaatcaagt atgaggatct ctttgtaaat ggcgaaagct agtaccctag
      541 gaaaaaatac tccagttcgg gatggaaccg ggacgtctat taaggattat acgagcctgt
      601 tgggtgtggt tgcggcaggc atattaggta tgagcttgtt atgtatcact tccgctattg
      661 cgctctcagc ccccaaccaa tctaaaccca tcgtactcga ggccggtaat attgtagatt
      721 tagccgtaaa taacatcaat accgtgtccg cactgacggt ccaggcgctc tcaacaatca
      781 gtatcgactc cacgccgata ttgctcccct ctcgtactcc gacttcgtca ccaacggtaa
      841 ctgcttcgcc agttttaatt cctcccacgc aaagtcagcc gacccgaacg cgtcgtcccc
      901 gaataccccc gacctttacg ccgcgaccgc ctcccacacg gacgcctctt ccaccgccca
      961 caaatactgc cgtaccaccg tccccgacat ccattccgcc gacgttcact tcaatacctc
     1021 caacgtttac ttcgatacct ccaacgcaga cctccgttcc tccgactgtc actccggaac
     1081 ctccgactgc cacgccgaat cctcctacag ttccgcctga caccagtacc ccagcagatc
     1141 cgccgccaac cgttcctgtt gtgccgatcg ttgacagttc gcctacctca ggtccctagc
     1201 cgagagattt gctgatctct taaatcaaac cgccttcaga gtgacaggcc ctgaaagcga
     1261 ttgctctata tgcggcctct ttatttcact tttaagttaa caactccatt aaaggcaacg
     1321 gcgcaccact taccccgttc aaattcaatc cacacactct tcgactgcat gcgcttgccc
     1381 gtcacttttg cacctttttg taactgacca acgattgcaa attccacccc ggggccattc
     1441 cgcacgttca aaaattcaga taatacttca aaagataagt ttggaagctt gattgcatct
     1501 gtgaactggt cagttgaagt tgtctcatcc gaatctgcag cgtcacccac cgtgaagagc
     1561 ggcatcgggt ctacatatcc tttgtaggcc gggttctgtc catcaatctt tacaccgaaa
     1621 tgcagatgtg gtcccgtgct tgcgccggtg ttattgctaa atccaatcat tgagcctact
     1681 tttaccttct gccccgggct aacggcagca ctcgccagat gcgcataata agtgaaatat
     1741 ggtttgcttc catccatgtg actcaatttg acgtagttgc cgtacccccc gttttcaaat
     1801 gaaaccgccg ctactgtgcc cgacgccgca gcgcccactg gcgttccatt tgctattcca
     1861 taatcgatac cgttgtgtcc ggggaatccc cactgcttat accgttcagg attctcaccg
     1921 aacaactgcg tgattggacc attgaccggg acaaccaatt tgatagccat tgtttatctc
     1981 ctctgccatg tgagtggaat gccatcctga aaacataata acacatcctt attaggacaa
     2041 tcctgttaat tgatacgagg caatgccgaa ggcgatggcg acattaaacg atgtcttctc
     2101 gccgcgcatt gggataaaaa atattctttc ggatagctcg atcaacccag gatcaacgcc
     2161 cgttacttcg ttgcctacga tcagaattac cgggtttgta gaatcggcat caacatgttt
     2221 tgataaatga acggcccgtt catgttcttc caaagcccag actttcccac cctctttctt
     2281 tagggcgccc acaagttgga ctgcgtcctt atgataagac catgaaacgt aattttctgc
     2341 acccagcgaa gttttcgtca cggcttcgtt ttccggtgtg ggtgtaattc cgcataaata
     2401 cacatgtgcg aagccaaatc catcggcgct gcgaaagatc gaaccaacat tccacgccga
     2461 gcgaatgtta tccaaaagaa ccgagaccct ccgtctgctt tcagtggatc ggatagaggt
     2521 gaggttggtt ttgaggtcag tattgacgcg aaccgagagt tcagtttctc cgaggcagac
     2581 cgggcagcgc attccaaatg tgctttcgtt cgtcattgga tagcgcaatc cgcaggaaag
     2641 acacgtacgt atttgaaatg tatcaggagt cataatggga ttatacatgc tacaattaaa
     2701 gccactggat ataaagtgca tcgcgttgtc cctgatttga tcttagaaaa ttatcggcgc
     2761 ggactctatc atggggagtt tccgaccatc gcgttgtttt tggacctttc agggttttcc
     2821 acgatgactg atgttctgat gcagcaaggt caacacggcg ccgaggtgct ggcaggattg
     2881 atgcatacgg tgtttgatcc acttgtagat ggcatatttg catatggtgg gaagatcatc
     2941 agctttgcag gggatggcat tttggcgctc tttccgatcg agtcgagcga gaaatcctcg
     3001 gcgttgaccg cccttgctgc cgcctgggat attcagcaaa cgctaggcga gaatccagaa
     3061 catgagactg tatacggaaa atttcggttt tcggtaaaga tcggcctggc ttttggtaca
     3121 gtgtcgtggg gaatcctgca ttcacaagat cggcagcagg cgacgtacta cttccgtggc
     3181 actcccgtcg atgtctctgc agcagccgaa aagcatgcca gtgcagggga gatcttaatt
     3241 acagacgaac tcaatgaatt gctcaaaaat gaaattgagg cgactccgtt tggtcccttc
     3301 cataagctcc acggttttcg tgtggccatg ccggttccca cgcctacggc cctcgatcca
     3361 attgacctgg aagcagctcg tattttcatt cccgaaaaga tcattgttca ggacgtccgc
     3421 ggtgaattcc gacaaatcgt taatttgttc atgcgttttc ccgatctacc caacgaacag
     3481 ctagatcaat tcttctatat catatttgat ctacataaaa aatacggcgg tctgctaaac
     3541 cggctggact ttggcgataa ggggtgcaat atgctgatgc tgtggggtgc accggtcgcc
     3601 tatgaaaatg atatcaggcg tgcgctcaat ttcattctgg acctgcagtc gagagtaact
     3661 ttccctatta ctgcaggtat tacgtattac gttgcccatg caggttatct tggtagtgtc
     3721 atgtgtgaag attacacatg ttatggatgg ggagtcaatc tggcatcgcg ttttatgatg
     3781 tctgcacccc agggtaagat ttgggtggac gatcggattg caagacgtgt ggccactcgc
     3841 tttgagattg aaaatgtggg tgagcaacgg ttcaaagggt tttctgcgga acaaaaagtg
     3901 aatcttctga aaggtcataa gcgggaagca gaagcgatct atctgggtga gttagtcgga
     3961 cgcgagaacg agctcgcggc ccttgaatct tttacggatc cactttggaa tggaaatttc
     4021 gcgggcatgt tgctggtatt gggagatgcg ggcacaggga agggaagact cgtacatgat
     4081 tttcgtgcct cagaagtatt cgagaagcat tacgcgctct gggcactgtg ccaggctgac
     4141 caacttgttc ggcagtcatt taacccattt cgcagttggc tgtttcggta ttttggattt
     4201 gcatctgcta acaatgttga cgaccgaaaa cagatattcg tttctaaact acaggcacta
     4261 agggcagcga ttcaggacga aacgctcgca caggaattag atcgtctgca ctttgcactt
     4321 ggtgcgctgc tggacctgcg ttggattgat tcgccatacg aacaaatgga cgcggagggt
     4381 cggcataaca atacaatcct ggggctgatc gcgctaatta aggcggaaag cttaagacag
     4441 cctttgatac tgttcatcga ggatatgcaa ttcattgatc acgatacgct ccagtttttg
     4501 ccccgcctga agcgtacgct acttgccggg caggagtcgt atccggttgg tatcatcgcc
     4561 acctctagag tacagccagg taatccaatt acagaaggtt tgattgactc ccaaattgag
     4621 ctcggcgggc tctcccttga agcggttgcc cgcctcattg aaattcagct tggaggggtc
     4681 cctgcggtcg agttggtgaa gctggtgatg gcgcgttcag agggaaaccc atattttgtc
     4741 gaacagatca tccgttatct gcaggaggaa catgttattg aaatgagcga gagagggtgg
     4801 aatcaagtca ggcggacgcg cgattttttc gttcccagcg atcttcgcgt ggttttggta
     4861 gcgcgtcttg accagctaac ccaggaagta aaagatgtag ttcagacggc ctctgtgctg
     4921 ggacgagaat ttgcaatttc agttctgacc gaaatgaccg atgaagcaga taatatcgaa
     4981 cgtgtcgtgg ctgatgcggt gcaatccgaa atctgggcac cccaggatga agtgcgttat
     5041 gtatttacgc atggcttgct tcgtgatgcg gcgtatacca tgcaaatgcg tgctcgcaga
     5101 caggagctac acaagtgggc tgtcctggct ttggaaaaga tttatgccga cgaactgaaa
     5161 tttcattacc ctgaactggc atatcatgcg gagcgggcta acttacatca gaaggcgcag
     5221 gaatatttct tattggctgg ccaggcctct gcggaggtgt acagtaacag ccaggcaatt
     5281 gattatttta cccaagctct cacgtttaca cctgctgacg atgtggcagg tacattcgag
     5341 ttgctggcag aaagagccgc cttattccat cgtgtgggag atcggatccg tcaactgcgt
     5401 gatattgagt ctctggaaca attggcaaaa gaacccaccg atcttaacgg aatggcacgc
     5461 gtcgagatgc tcaaagcgta ttattgtttt gccacaggtg attatctttc tctgattgac
     5521 catgccaggc gcgtcatgga cctgaacatt ccgatgaatg ccgagatggc aatggatacg
     5581 tattcgatct ggtcgcttgg cttacttcgc cttggaagac tcgatgcagc aatggaaatc
     5641 gctcaggacg gactggatct tgcgcaggct tcgcgtaata tccctaagca gggcagtatc
     5701 ttaaattcta tgggtttgat cgcgctggag cagaagaacc cagccgtagg tcgtggatac
     5761 ttcgaactgg cactgtctat tgcacgagag gtaagtgatc aacgacttga ggcgcagtgc
     5821 ctgaacaatt tgggtaactc tgccgggttt gtgcagggtg attattcagc cgccatgcaa
     5881 tactatgagc aggcaaacgt gatctttcgc gaacggggtg atcgttcctc tgagggcatg
     5941 accctaaaca atctgggatg ggcggccgga atgttgggcg attttatctc ggcacgtggg
     6001 cataacgagc gagctttgct tattgcgcgc gaagtcggca accgatacca ggaaacctac
     6061 acgcggatca atctgagcgc ggtaacgggc attgatggag atgcgcaagc ctcgttggat
     6121 tatgctcaca cagcacagga attggcaatt gcggctggag aactttcagg ggaggcctgg
     6181 gcgttgttgt ataaaggaca tgccctgcta atgttaggcg actctgagcg tgccagagaa
     6241 aatttca
//
LOCUS       DASTJP010000802         9521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_494871, whole genome shotgun sequence.
ACCESSION   DASTJP010000802 DASTJP010000000
VERSION     DASTJP010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494871"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="VFQ23_23440"
     CDS             complement(<1..1753)
                     /locus_tag="VFQ23_23440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909621.1"
                     /translation="MSWFQYLKQHLSNLGSSRLTKPSVFEYAQLVLGILVFYLLLVFP
                     PAPFTNKIVVDPNNRIVFLCLAVFCILLFSKTGSAWKIIQASLLFALFAMTLIYKWQS
                     AHYDGFMIGGMLPWSDSAAYNWEAHRLVNGNLLSTWGGRRPLFSGLLAVLFQLTGDFM
                     LTLSILTLFNALAVFFVVRIVNKLYGSVAAGTFLALSLVFYFRFSGVTASEQLGFAAG
                     NLALYFLLIGVDTRNLKMALFGLGLLTLALNARAGAFFILPVLIVWLASSFRQTIAFW
                     RSAGLAVLVVSMVFVLNLVLVRIIADPTSTSFSNYSYTLYGLASGNKGWNQVIKDYPD
                     VTESEVMPLAIEKIRTAPSLIVKGTFRALGDYFRIDYGAFSFIYRGVFKQRVNLILWG
                     LVALGLAYSLSRWKEGFHGFILASFLGVIFSVPLLPPIDSDKMRVYAATIPFSVLWIA
                     TGVSALFNWRRTMIVRTENQAETETELLVQHALLISILFLGLILSALFLRHNFLPGSP
                     RQSSTPVCISGEEVIKGMKFRNTSIFIVEDGAIPESYAPFIRQADFQRAIGKAPPLYP
                     FLNDELFRLTSGDGVSVG"
     gene            complement(1755..3695)
                     /gene="asnB"
                     /locus_tag="VFQ23_23445"
     CDS             complement(1755..3695)
                     /gene="asnB"
                     /locus_tag="VFQ23_23445"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9909622.1"
                     /translation="MCGITGFWNTEKLQTNRDLISTVTRMRDIMHHRGPDDSGEWVDE
                     TSGIALGFRRLAIVDLSPTGHQPMFSADQRYVMIYNGEVYNYAELRAELDAAGYQFRG
                     TSDTEVMLAGILEWGLERAVTRFNGMFAIALWDRQEKKLHLIRDRVGVKPLYYGWCGR
                     TLLFGSELKALNAHPSFKRAIDRQSLTLYLRYGYVPAPHSIYQGVKKLEPGNILTFDE
                     GMSQTEGSSLRYWSAREVVERGLQNPFLGSDQDAISELDHLLRASVRDRMIADVPLGA
                     FLSGGIDSSTVVAMMQVQSARPVKTFTIGFQEAGYNEAEYAKEVAAHLKTDHTELYVS
                     PRDAMDVIPQLAFMFDEPLGDSSQIPTYLVSKLARESVTVSLSGDGGDELFGGYNRYF
                     NSLNVWSLVGNIPFPVRKVAGAVFDQASKVEWHKITSHGRLSTRFYHLSEITKMRSAD
                     ELYWRFSSNWEQPRQVVVDGREPPAFHMAPENAHLLPEFAQRMMYLDFNTYLPDDILV
                     KLDRASMATSLEGRVPLLDDHRVVEFAWRLPLHMKIRSGKGKWILRQVLHQYIPQKMV
                     DRPKMGFGVPIDSWLRGPLRDWAESLLDETRLRQEGYLNPGPIRKRWTEHLQKQKDWQ
                     YPLWYVLMFQDWLGHGEKNLIT"
     gene            complement(3695..4873)
                     /locus_tag="VFQ23_23450"
     CDS             complement(3695..4873)
                     /locus_tag="VFQ23_23450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9909623.1"
                     /translation="MNSILFLLRSFSVGGAERQLCVLALGLQQKGYSVKVAVIYTGGA
                     LEAEARKMGLQVVDLKRKGRWDLIPFFFRLIRLVKSEKPDILHSYLQVPNIWSAFVKI
                     LQPRTKVVWGVRSSDIKWKDYGLQWQLTDMVESLLRKIPDWIILNSHAGLLHHAKLGY
                     PSQKMSVVHNGIDTRAFFPDRKLGHALRAQWGIRDNQKLIGMAARVDPVKDYPAFLRA
                     ASLLLQARQDVRFVCVGGGSSDYVRECRELAASLNLDDFLIWAGERADMLNVYNAFDI
                     LVLSSVSEGVSNVIGEAMACGVPCVVTDVGDSAQLVGSLGEIVPSGDSEALKQGVLIM
                     LDRLANDEELSERLSQRIIEQFSIAKLVSRTVTILDEVAKIDGVNRPAINFFSRKNDH
                     "
     gene            complement(4926..5729)
                     /locus_tag="VFQ23_23455"
     CDS             complement(4926..5729)
                     /locus_tag="VFQ23_23455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9909624.1"
                     /translation="MSTDSMRFGFGKNWDDYIKKHFSEERVEISRKHLLSFLKLENLE
                     GKSFLDIGCGSGLHSLAALRSGAERIYSFDYDPDSVATTQKLREFAGTPSYWEARQGS
                     ILDKEFLGTIEPADVVYSWGVLHHTGSMWEAMDNVVPLGKKGSLYYIALYDYEIHVDP
                     TPEFWLDVKQRYNKSSAWGKRKLELWYIWRFMLYGSIRNVPQFLGRIFGYKQSRGMAF
                     YNDVKDWLGGWPMEFAKRPDVKSWADKHGLEMLTMKTGEANTEYLFRKL"
     gene            complement(6270..7388)
                     /locus_tag="VFQ23_23460"
     CDS             complement(6270..7388)
                     /locus_tag="VFQ23_23460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9909625.1"
                     /translation="MKPRGMMVIHQFRPITSGAELQAERLAIRLVQRGHEMQVFTELR
                     VPGSLPEEDIQGVAVHRVDFKMAYWILTGAESTFRYLVKTRKSYDVLHVHQAFGHAVV
                     SIIAAKVFGKRCIVKIACAGSYGDLNFFSDFPGFKGALSVLKRADCIIALSSDVEREL
                     LEWGFAPQRIKRIPNGVDTEFFKRERPFPPRAPVRFILIGRRTPQKGIDLALQAAQIL
                     QKEGLDNEFEIKFYGLDYTEYDYRQMAEELGVTSLVEFLPHQDAIRDILHQAHSLILP
                     SRAEGMPNVILESMSFELPVIASRVSGVVDIIHDQVDGILIPEEDPQALAHEMKRIIK
                     DPELATKLGRQARQKVASEFSLESIADKYSDLYEQLWS"
     gene            complement(7390..9318)
                     /gene="asnB"
                     /locus_tag="VFQ23_23465"
     CDS             complement(7390..9318)
                     /gene="asnB"
                     /locus_tag="VFQ23_23465"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9909626.1"
                     /translation="MCGICGIIYHDPRRRVSNELVTQMNNVIHHRGPDDSGEYINGNV
                     GLGFRRLAIIDLSPTGHQPMTNEDGSVWIVFNGEIYNYQELREELLAKGHKFNSRTDT
                     ETILHLWEEEGERCVERLRGMFALALWDSKQQCLFLARDREGKKPLFYAQLADRLLFG
                     SEIKTILQDPDFHPSPNLEAIHLYLAYQSVPAPYSAFEGIKKLPPAHTLLYKNGRVKL
                     RRYWKLSYKEKRVIRTPQDEIRLQEEIIDRLREAVRIRLMSDVPLGAFLSGGIDSSII
                     VALMAELMDQPVKTFSIGFTHEEYNELPYARQVAERYGTEHHEFVVTPDAQAIFPELV
                     WHYNEPFADSSAIPTYYVSKLAREFVTVILSGDGGDENFAGYPRYQNTGDYALNGTPQ
                     TLFHRLFRLNGMAQGFVNQGSSWYKTFTRLKDLDQQKLLYYYRITHFHEQYQSKLYTQ
                     DFWKKLNGVSTVDWMLDIYRQSDAHNFLDATLDLDLRLYLPDTLMTKTDIASMAHSLE
                     ARAPMLDHEFLEFVASIPPELKLKNGTESKYIFKKAVEPYLPHEVIYRKKMGFGVPID
                     HWFRHELRDMVRDTLLSQRAMQRGYFQRDYIEQILDRHQSGGESWQYMIWNLLMLELW
                     HLMFIDQTMPIPTEHAAV"
BASE COUNT         2604 a         2449 c         2146 g         2322 t
ORIGIN      
        1 atccaacgga tacaccatca ccggatgtaa gccggaagag ttcatcattc aggaatggat
       61 atagtggagg ggccttacca atcgctcttt gaaaatcagc ttgtcggatg aagggtgcgt
      121 aggattccgg tatcgcacca tcctcaacaa tgaaaatgct ggtattcctg aacttcattc
      181 ccttaatcac ctcctcccca gagatacaaa caggagttga agattgacgc ggggagccgg
      241 gaaggaagtt atgtcgcaga aagagcgcag ataagatcaa acctagaaaa aggatggaaa
      301 ttaataaagc atgttgaaca agtaattcag tttcagtctc ggcctgattc tcagtcctta
      361 caatcatcgt tcggcgccag ttaaataacg cagatacccc ggttgcgatc cacaagactg
      421 aaaaagggat tgtggccgca taaacgcgca ttttatcgga atcaatcgga ggcaacaaag
      481 ggacagagaa aataactcca agaaaggagg cgaggataaa accatggaat ccttccttcc
      541 atcttgaaag tgaataagcc aatcccaagg cgaccagtcc ccaaaggatc aggtttaccc
      601 gctgcttgaa cacgccacga tagataaagg aaaacgcccc ataatctatt ctgaagtaat
      661 cccctaatgc ccgaaaggtg cctttgacaa tcagacttgg cgcagtgcgt attttttcga
      721 ttgccaatgg catgacttct gattcagtca catcagggta atcctttatg acctgattcc
      781 agcccttatt gccgctcgcc agcccatata atgtgtagga ataattggaa aaggaggtac
      841 ttgttggatc tgctatgatt cgtaccaaaa ccagattcaa cacaaagacc atgctgacaa
      901 ccagaactgc aagacccgca gagcgccaaa aggctatcgt ttgacgaaag gaagatgcca
      961 gccacacaat gagaactgga agaataaaga aagcacctgc acgggcattc aaggccaggg
     1021 tgagtaaccc cagaccaaag agagccattt tgaggtttcg ggtatctacc ccaatcagaa
     1081 ggaagtaaag tgctaaattc cctgctgcga agcccagttg ttcagaggcc gtcacaccgg
     1141 aaaaacggaa ataaaacacg agggataggg cgagaaatgt tccggcagcg acagagccgt
     1201 aaagcttgtt aacaattcga acaacgaaaa aaaccgctaa agcattgaac aaggtcagaa
     1261 tactcaaggt gagcatgaag tccccggtaa gctgaaacag gacagccaga agtcccgaga
     1321 acagcggacg acgaccaccc caggtgctca ataggtttcc attgaccagc cgatgagctt
     1381 cccaattgta ggcagcagaa tcggaccagg gaagcattcc cccaatcata aatccgtcat
     1441 aatgagccga ttgccattta tagatcagag tcattgcaaa caaggcaaat agcaatgagg
     1501 cttgtattat cttccatgcg ctgccggttt tactgaacaa taggatacaa aatactgcca
     1561 ggcaaaggaa cacgatcctg ttatttggat ctacgacgat tttattagtg aacggcgccg
     1621 gtggaaacac caacaataaa tagaagacaa ggatgcctag taccagttga gcgtactcaa
     1681 agacagaggg ctttgttaag cgactcgaac ctaaattaga aagatgttgc ttcaggtatt
     1741 gaaaccagct catatcaagt tataagattc ttctcaccat gtccgagcca gtcctggaac
     1801 attaacacat accacaaagg atattgccag tccttttgct tttgcaaatg ttcggtccat
     1861 cgttttcgga tgggcccggg attcagatag ccttcctggc gaaggcgcgt ttcatccaaa
     1921 aggctttcag cccaatcgcg caagggtccg cgcagccagg agtcgatcgg cacgccaaac
     1981 cccatcttcg gacgatccac cattttctgt gggatgtatt ggtgtaatac ctgccgcagg
     2041 atccacttgc ctttccctga gcggatcttc atgtgaagtg gaagacgcca ggcaaattcg
     2101 acgacacgat gatcgtccaa aagtggcact ctgccctcca gactcgttgc catacttgcc
     2161 cggtccagtt tgaccaaaat atcatccggc agataggtgt tgaaatccag atacatcatc
     2221 cgctgggcaa actcaggaag caggtgagcg ttctcgggcg ccatgtgaaa ggctggcggc
     2281 tcgcgaccat caacgacgac ctgtcgcggc tgttcccagt ttgagctgaa tctccaatac
     2341 aattcatccg cacttctcat cttggtgatt tcagacaaat gataaaacct ggtagatagc
     2401 cttccatgcg atgtaatctt atgccactcc actttcgagg cctggtcaaa aacagcgccc
     2461 gccaccttgc gcacagggaa cggaatattt ccgacaaggc tccagacgtt caatgaattg
     2521 aaataacggt tatatccgcc aaataattcg tcgccgccgt cgcctgaaag agaaacagtc
     2581 acagactctc tggcgagttt ggaaaccaaa tacgtcggaa tttgagatga atcaccaagc
     2641 ggttcatcga acataaaggc cagttgggga atcacatcca tcgcgtcgcg cggggagacg
     2701 taaagttccg tgtgatcggt cttgagatgc gcagctactt cctttgcata ttcagcttcg
     2761 ttataacctg cctcttgaaa accgatggtg aacgtcttca ccgggcgtgc gctttgcacc
     2821 tgcatcatcg caacgactgt tgaggagtcg atacctccag ataagaaggc gcccagaggc
     2881 acatccgcga tcatccggtc ccggacggag gcgcgcagca aatgatctaa ttcagagatc
     2941 gcgtcctggt cacttcccaa aaatggattt tgcaacccgc gttcgaccac ttcacgcgca
     3001 gaccagtacc tgaggctgct tccttccgtt tggctcatcc cttcatcaaa cgtcagaata
     3061 ttgcccggtt ccagtttctt cacaccctga taaatcgaat gtggcgcagg gacgtaacca
     3121 tagcggagat aaagcgttag cgactggcgg tcgatcgccc gcttaaagct cggatgcgcg
     3181 ttcagggcct tcaactctga tccgaagaga agagtccttc cgcaccagcc ataataaagc
     3241 ggtttgactc cgacccggtc acgaatgaga tgaagttttt tctcctggcg atcccataag
     3301 gcaatcgcaa acatcccgtt gaagcgcgtc acagctcgtt ccaagcccca ttccagaatc
     3361 ccggccagca tcacttcggt atccgatgtg ccgcggaact gataaccagc cgcatccaat
     3421 tcagccctta actccgcgta gttataaact tcaccgttgt agatcatcac ataacgctga
     3481 tctgcagaga acatcggctg gtgcccggtt ggagataaat cgacgatcgc aagacgacga
     3541 aagccgaggg caataccaga tgtttcgtcc acccactccc cgctatcgtc gggaccccgg
     3601 tggtgcatga tatcgcgcat gcgcgttact gtggagatca gatcacgatt cgtctgtaat
     3661 ttttccgtat tccaaaaacc ggtgataccg cacatcaatg atcgttcttt cttgaaaaga
     3721 agtttattgc aggtctgttc accccgtcta tttttgcaac ttcatcaagg atcgtaactg
     3781 tcctggaaac caattttgca atactgaatt gttcgatgat cctttgtgag agtctctcgc
     3841 tgagctcttc gtcatttgca agacgatcca gcatgattaa tactccctgt tttagggctt
     3901 cggaatctcc cgaaggaacg atctcgccca gagatccgac caactgagca gaatcgccta
     3961 catccgtcac aacacagggt acgccacaag ccatagcttc gccgattacg ttagaaacac
     4021 cttcactgac cgaagataat acaaggatat cgaaagcatt ataaacattc agcatatccg
     4081 cccgttcacc agcccaaatt agaaagtcat ctaaattcaa ggacgcagca agctcacgac
     4141 attcccgaac gtaatcactg gagccgcctc ccacacacac aaaacgaaca tcttgacgcg
     4201 cctgtagcag caaagaagca gcgcgtagaa aagcaggata atccttgacg gggtccacgc
     4261 gcgccgccat gccaataagt ttttgattat ctcgtatgcc ccattgagct ctcaaggcat
     4321 gcccaagctt tcggtccggg aagaaagcgc gggtatcaat tccgttgtga actacgctca
     4381 ttttttgaga tgggtatccc agttttgcgt gatgaagcaa ccctgcatgt gaattaagaa
     4441 tgatccaatc tgggattttc cttaacaggg attccaccat atccgtcaat tgccattgca
     4501 atccataatc tttccattta atatcggagg agcggacacc ccatactact tttgttctag
     4561 gttgtagaat tttgacgaag gctgaccata tgtttggtac ctgcaaatag gagtgcagga
     4621 tatctggctt ttcgcttttg accaatcgta tcaatctgaa gaaaaaaggt attaaatccc
     4681 agcgtccctt tcttttcaaa tccacgacct ggagccccat ttttcttgcc tctgcttcca
     4741 gagctccgcc ggtataaatc acagcaacct tgaccgaata gcctttttgc tggagtccca
     4801 gagcaagaac acacaactgg cgttctgcac cacctaccga aaaggatcgt aaaagaaata
     4861 ggatgctatt catactgttt ttggcaacag aacctggtta ttgaccttca ggcaaaatac
     4921 tcttcctaca actttctgaa taaatattct gtatttgcct cgcctgtctt catggtcagc
     4981 atctccaggc catgcttgtc ggcccaactc tttacatcag ggcgtttggc aaactccatc
     5041 ggccagccac ccagccagtc tttcacatcg ttgtaaaatg ccatcccgcg tgattgttta
     5101 tatccaaaaa tacgacccag gaactgaggt acgttacgaa tacttccata cagcataaat
     5161 ctccagatat accagagttc cagttttcgc ttgccccagg cactactttt attgtaacgt
     5221 tgcttcacgt ccagccagaa ctctggcgtc ggatcgacat gaatttcgta atcatatagg
     5281 gcaatgtaat acaaactgcc tttcttcccc agcgggacga cattgtccat ggcttcccac
     5341 atgctgcccg tatgatgaag cacgccccat gaatagacga catctgccgg ttcaattgtg
     5401 ccaaggaatt ccttgtccag aattgacccc tgccgcgctt cccaatagga gggcgtcccc
     5461 gcaaactctc tgagcttttg cgtcgttgct accgaatctg gatcgtaatc aaaactatag
     5521 atcctctccg ctcccgagcg caacgccgcc agcgagtgta agccacttcc gcagccgata
     5581 tccaggaagg attttccctc gaggttctcc agctttaaga acgacaacag gtgcttgcgc
     5641 gatatttcga cccgttcctc actgaaatgt tttttgatat agtcatccca gttcttacca
     5701 aaaccaaatc gcatcgaatc tgtagacatg tctatattct cctcatggat tcgtagttat
     5761 acaaggtgat ccagccttct cctgccagat taacgttgac catctttagg ctgggaaata
     5821 acataaagaa aagagagcca ataaaatgaa ctccaaaaaa agaacgagaa tttcaggcgt
     5881 cttaatgatc ctgcaatctg cccgagcccc gagaattttt taagtggacc aaaaaaaact
     5941 gattggggaa atagctttag gatccagtgc aatggaaaga gcaaaatacc caacctggtt
     6001 atttcattct catcatagcg aaacgaatat gaccgaccat ttgttctggg gtcactatta
     6061 tcggatgctg aaaagtaaac ctctccgtac caaaaagaat aacgacccat ccccaggttg
     6121 cgaatcgaat cgtgggaagc acgagctgca ccaggcaagg gcacgccgtc ctcaagcata
     6181 attgcggagc ccaatccaag cttcttcgtt cgagcaagat acgcaaatcc accgttatgc
     6241 cgaattttgt ttacttcctg tatcttcatt caactccata actgctcata gagatcagaa
     6301 tacttgtcgg caatgctttc aagtgagaat tctgatgcta ccttttgcct ggcctgtctt
     6361 cccaatttgg tggcaagctc aggatcctta atgattcgtt tcatttcatg tgcgagggcc
     6421 tgcggatctt cttctgggat caatattccg tccacctgat catgaataat atccacaacg
     6481 ccactcaccc ggctggcaat gaccggaagc tcaaaggaca tgctctccaa aatcacattt
     6541 ggcatcccct ccgcgcgact cggcaaaata agtgaatgtg cctggtgcaa aatatcccga
     6601 attgcatcct gatgcggcag gaattctacc agagaggtta cccccaattc ttcagccatc
     6661 tggcgatagt cgtattcggt gtaatccaat ccatagaatt tgatttcaaa ttcattatcc
     6721 aacccttctt tttggagaat ttgcgcggct tgcaaagcca gatcgattcc tttttgaggc
     6781 gtacggcggc cgatgagaat aaatcgtacc ggtgcgcgtg gaggaaacgg tcgttctctc
     6841 ttgaaaaatt ctgtgtcaac gccattcgga atgcgcttaa tccgttgagg agcaaagccc
     6901 cactccagca actctctttc tacatcactg ctgagggcaa taatgcagtc ggcacgtttt
     6961 agcacggaca gggctccttt gaatcctgga aaatcggaaa agaaatttag atcaccatag
     7021 cttcctgcgc atgcgatctt cacgatacat cgcttcccaa agactttggc agcaatgatc
     7081 gagactaccg catgaccaaa ggcctgatga acgtgcaaga cgtcatagct ttttcgggtt
     7141 ttgacgaggt agcgaaatgt gctttccgcc cccgtcaata tccaataagc cattttgaag
     7201 tctacgcgat ggaccgccac gccctgtata tcttcttcag gcaaagagcc aggtacgcga
     7261 agttcagtga atacctgcat ttcgtgtcca cgttgaacaa gccgaattgc cagtcgttct
     7321 gcctgcaact ccgcgccgct cgtaatgggg cggaattgat gaatgaccat catacctcgt
     7381 ggtttcattt tagactgctg catgctcagt agggatgggc atggtctgat caatgaacat
     7441 caggtgccac aattcaagca tgagcaaatt ccagatcatg tattgccagg attccccgcc
     7501 tgattgatgc cgatcgagga tttgctcgat atagtctcgt tggaagtagc cgcgttgcat
     7561 cgctcgctgg ctcagtaagg tatcccgcac catatcacgc agctcgtgac ggaaccagtg
     7621 atcgattggc acaccgaacc ccatcttttt gcgataaatg acctcgtgtg gcaagtacgg
     7681 ttcaaccgct tttttgaaga tatatttact ttctgtgcca tttttcaatt tcaactccgg
     7741 gggaatactc gccacaaatt caagaaattc atggtcgagc atgggtgccc gggcttcaag
     7801 cgaatgcgcc attgaagcga tgtcagtttt tgtcatcagc gtgtccggca gatagagcct
     7861 caagtcaaga tcgagggtag catccagaaa gttgtgcgca tctgactggc gatagatatc
     7921 cagcatccaa tcgactgttg aaaccccgtt tagctttttc cagaaatcct gagtatataa
     7981 ctttgactga tattgctcat ggaaatgagt aattcgataa tagtacaaaa gcttttgctg
     8041 atcgagatcc ttgaggcgtg tgaacgtttt gtaccagctg cttccctggt tgacaaatcc
     8101 ctgtgccatc ccattgagac gaaacagacg atgaaacaaa gtctgagggg ttccgttgag
     8161 agcgtagtcc cctgtgttct gatagcgcgg atacccggca aagttttcat cgccgccatc
     8221 tccgctcagg atgacggtaa caaattcacg cgccagcttg gaaacataat aggtaggaat
     8281 ggcagaggaa tcagcgaacg gctcattata gtgccacacc aactcaggga aaattgcctg
     8341 cgcatcaggc gtgacaacga attcgtgatg ctccgtcccg taccgttcag cgacctgacg
     8401 tgcgtagggt agttcgttat attcctcgtg ggtaaagccg atcgaaaacg tcttcaccgg
     8461 ctgatccatc aactcagcca ttaaagctac aatgatcgat gaatcgatgc cacccgagag
     8521 aaaggcaccc agcggcacgt cactcatcag gcggatgcga accgcttcac gcagccgatc
     8581 gataatttct tcctggagcc ttatttcatc ctgcggggtt ctgatgactc gtttctcttt
     8641 ataggataac ttccagtaac ggcgcagctt aactcgaccg ttcttgtata acaatgtatg
     8701 cgcaggagga agcttcttta tgccttcaaa cgcgctgtaa ggcgcgggaa cagactgata
     8761 ggcaaggtaa agatgaatcg cttcgaggtt gggagaagga tgaaaatcag gatcctgcaa
     8821 aatggtcttg atctctgatc cgaacaacaa gcggtccgca agctgggcgt aaaatagcgg
     8881 tttcttgcct tcccggtcac gcgccaggaa caggcattgc tgtttcgaat cccagagagc
     8941 cagggcaaac atgccacgca agcgttccac acagcgttcg ccctcttctt cccataagtg
     9001 aaggatcgtt tcggtatcgg tccgagaatt aaatttatga cccttggcaa gaagctcttc
     9061 gcggagttcc tgatagttat agatttcgcc gttaaagacg atccagaccg aaccgtcttc
     9121 attcgtcatc ggctgatggc cggtcggaga aaggtcaatg atggcgagac gacgaaagcc
     9181 cagcccaaca ttgccattga tatattcacc gctatcatcg ggcccacgat gatggatgac
     9241 gttgttcatc tgcgtaacga gttcatttga aacccgccgc ctcggatcat gatagattat
     9301 tccgcaaatt ccgcacataa gacctacctc tttcctttac ctattataag gagtatacat
     9361 agtttgcaac tattccggcc agatgacttt ataagttctg ccattggaat tgggatctga
     9421 accgtcagat gtagagaaaa acaaaaattc cccccagtgg gaataacttc ccccaccttt
     9481 tttacgaata tctgcgtgat tcgtatgggc ggggcccaat a
//
LOCUS       DASTJP010000803        11440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1023862, whole genome shotgun sequence.
ACCESSION   DASTJP010000803 DASTJP010000000
VERSION     DASTJP010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11440
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023862"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..628
                     /locus_tag="VFQ23_23470"
     CDS             251..628
                     /locus_tag="VFQ23_23470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909627.1"
                     /translation="MATNTLHEKDLHRVFHEIRQEIGNAQSREELTELYKRTGYMITM
                     THATPVEEKQDTEMKDRRETTEHEFATTVRLINSRAQELGVEADYNENWDGLAANGYE
                     AEGENLLEAEENEQFDEGEVTTR"
     gene            791..1240
                     /locus_tag="VFQ23_23475"
     CDS             791..1240
                     /locus_tag="VFQ23_23475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909628.1"
                     /translation="MIDVAQLPILLKAIDFIFDEGRKILEERRERRKAEAALQETQNP
                     EPAEEAAPVEEQEPAEVKQELVSSKIDEIVWKDHEAEVKHLVKLLETYSHNYHLAKEQ
                     YAKWGSALVPPIILHNMTEAENSMLETIQKLEGVLSKVYKKEINPVK"
     gene            1255..3486
                     /locus_tag="VFQ23_23480"
     CDS             1255..3486
                     /locus_tag="VFQ23_23480"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9909629.1"
                     /translation="MDKEEINHLETMISLHKKNLYILEEMLAKYGVDQPLHLVNSVTM
                     EREAIARYTKQIEGLTFKGREVEKSTALASLPRRPYFVGREEEIKTILQSLQPNSRTF
                     IIGIEGIGGVGKSTLATEVSHLCIENELFEAVIWVSAKEFTLTLHGIESITAEVKSLS
                     DILITIGTNLGNPTIGNLSIQDQIKRAYNLLSRQTTLLVLDNFESLSKNEQRDILDFL
                     RRSPITLKVVVTSRERVSEGQIIRLQGLSFEESDALLKWDAQQKNIHLTKDQSKHLVE
                     LTGGLPLALLWVQGQIAVLGYSVTQVLDKLSLDSDIPILQYCFNHSWNLLRHSNEKDL
                     LFILALQPDAVSRAALKEISELEDADAFDRSISDLLQLSLIEHEQNRDYFNVLPLTRR
                     FIRTQFASDRKFIKQAELKTAQYYVKYLPQKSSFKEWRGYDDLLRDRNNILSVAQWCY
                     KSIQKKQNTRNPLTKRAKNIAEILVQIGRQFGSVLWQRAYWYDRMTLAHAALNAAKLL
                     SDWKSVSTFARNISWIYFYQGDYLRALHWSEESLSATTKTEDELLIAAAKRSLGTVEM
                     RLGNFDRSQKLLEEVIQTTEQFTNDDYGIYSKGFAQYGLGDLENERGNTSKARDWYQK
                     ALDTWQNPVRNDPVRHISYALNGLGFVALKEKNYEDAKRLFMKGIQSAEEFGRSDELA
                     KGQLGLASVYFETESDLKSALTLVNEAIEIFQQHGMQYEIQKSRSLYDEILKVQTQIT
                     APR"
     gene            3494..5200
                     /locus_tag="VFQ23_23485"
     CDS             3494..5200
                     /locus_tag="VFQ23_23485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909630.1"
                     /translation="MENEKEIFLRSFKDLENQLQSLPIADAQLQQVKGFLSSIQKNAE
                     TLALYDPITHVFNTRAREWMMPGVPLKGMAKIDIYDLRQANKVYGIATVDSELHKLAY
                     MLMSMFTLENGDSVHRSPGSDEFRIFSTSKTPKELRFLLTKPYLDQETESLLSWDFGT
                     GHSETEAENNLQKQRKTFRPIVIRQTILESHAEIPRHIEGNNSYQSWNEFNMPYERLA
                     DTIYSLNLPVAIEQQTLEKLQTLKKIIENLVTKEALTSTLNALGAKWYLDSSLVQSVA
                     LTDMLDMHEGNARFGSLAIDQELRRFSNMLSEHFPKADGFLLFRSERAGDEFKITSTK
                     LDTTQLEDKIRTVWQEDLHRGLLAWNYGVGRNAGEAHVHLYRNRVKEIETMAASNSNG
                     KATFIIVRPESDDYAGLFKRSVDAARLINGTPIIDLHLTIQAIRNVQDFAALKERLEE
                     YCLTLQPFEVRVRNIARMNVNNQQGRLWLLAEKTPMLEDMYNNLGKIAEEMGCESYPY
                     KSQDWLPHMKIVDLPEHTSTQIKDPTFGVGNGVTFTVNFFEWTVQKAYDHWELLDQFV
                     FP"
     gene            5217..6191
                     /locus_tag="VFQ23_23490"
     CDS             5217..6191
                     /locus_tag="VFQ23_23490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9909631.1"
                     /translation="MATILLVDATPSDRRSYMMLLGNFGHRFLEAENGAQALDIARAE
                     LPDLIITDILMPHIDGFTLVRRLRSEPLLTGVPVIFHTANYDVTEIHRLARSSGIQHI
                     LRKPAEPQEILRAVNESLKHPTQPSRLPQTGQLQREHLQLLADKLYQKVAELEKANEN
                     LRNFSLTDGLTGLNNRRGFMILATNLLKFARRANYSSCLLYIDLDSLKQINNTFGHAA
                     GDLAITHFARILNDTFRESDIIGRLGGDEFVILIIDASEGDLANMEARLQSNVDAHNL
                     QADSEHALAFSLGSIRVDANSTITMEALMSQADEAMYRHKQSRRRVPQ"
     gene            complement(6210..6629)
                     /locus_tag="VFQ23_23495"
     CDS             complement(6210..6629)
                     /locus_tag="VFQ23_23495"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9909632.1"
                     /translation="MSKSKPPLKVFLCYAHSDKAEVYKLYQRVAKEFGIETWFDAEKI
                     LPGQDWEHAIRKAILESDVVVICLSRQFNKLGGYRHKELKIALEKANLLPSQEIFIIP
                     VRLEKCALPGRLRRWQCVDLFEDDGYQKLLQALRKCL"
     gene            complement(6683..9010)
                     /locus_tag="VFQ23_23500"
     CDS             complement(6683..9010)
                     /locus_tag="VFQ23_23500"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HET9909633.1"
                     /translation="MKKYTGWLFDLYEHQTKGVVLWLIGEDGKRYSFHQDFETIFYAS
                     GSAQRLHELGVFLRKGYSRENLRLERVTKEDLFAGPQDVMGIAVSRPDLYKTVFKAVY
                     RVFSDLIFYDVDIPLTVRYAAAHDVFMMARCEVVAEDDNTIVHIHALDTLDDLDPKLP
                     RLRILTLKPDTDPSHAFPQYLVATFRKSYLRISMEKPPELINLLNAILVSFDPDVIRT
                     HFGDAWLFPYLREVSKKYRIPFNPNRDVSMPVLRRREISFFNYGQAHYRAPQVHLRGR
                     WHVDLKNCMTYNQYQLIGAIEQTRLSSLPLQEVARRSPGAAIASMQNLTALKRGTLVP
                     YQHQKGEIPKTFHQFYQAARGGLIFQPAPGIFQNVAILDFSSKMASIMIKYNVSPETV
                     VRLHEAGEGLEISELGVKILSRPGLIPQTLRSMRDKRLSLKRRLKSMDKNDPKTRGIY
                     HRYKAVTNALKWLTVVCYGRLGFANSIFGRLNAHEVVSHLSRKVITDAKFLVEARGFD
                     VLHAYVDSLFVSRADASQEDFQTLANEIEQATGLPMEFENVYSWFAFLSSRQNPRIPV
                     ANRFYGIAENGEHKIRGLAVRRADTCKFVTDLQLQIIRTLAKESDPIKLMGLLPEIIE
                     ITQEKLDQLKNRIVPLRELTVTQTLSRELNNYSVLSPLAVAARQLKAQGKTIKMGQQL
                     KFIYIRGGPGVHAWDLPVPPDPTTIDITQYKELILRAVHEVLQPLNLTEKTLRDWLFN
                     KARYSTSPGLIAPGNLGRSELPLFNDLKHVYVDNF"
     gene            complement(9007..9300)
                     /locus_tag="VFQ23_23505"
     CDS             complement(9007..9300)
                     /locus_tag="VFQ23_23505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909634.1"
                     /translation="MAAKNLSPTELAHKIIAELMPLADMLSPSDRRIIKRFCEYILQN
                     RVSISNATSLLPLEAALVVVQVEEHENNNYQFSDLRSQLLELKREIEELKLKT"
     gene            complement(9310..9909)
                     /locus_tag="VFQ23_23510"
     CDS             complement(9310..9909)
                     /locus_tag="VFQ23_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909635.1"
                     /translation="MDNLHTHGFLPIRRSNHPFRAAHWINTYRFGHLLLVETSNEESN
                     VIVEVITQLALRGPFNLIAGDEWLPDRDTLYRSIRWHTLAVEEALNHPSLMRPMTCLQ
                     LLDLLMEVDMQNKPTLILNFLHHFYNADVELSLRDRVLERCCQYTKRLALSNSVVVLV
                     PRLSTMEYRRFFPVLASIANEIIPLKEIPSANAIQNLLF"
     gene            complement(10638..>11440)
                     /locus_tag="VFQ23_23515"
     CDS             complement(10638..>11440)
                     /locus_tag="VFQ23_23515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909636.1"
                     /translation="FLILSMAGIVAAWVYNEPLTLEATNRLTEIDTQLSQAQTSLQSS
                     QMELERALRILNAAEDAMEKLAGQSTSAENIFDNIQDTLDDRLLPELKTTRERILAAR
                     AALENLRNALEGVSSFLSFDLNLPDEILADLITSANLLDAEISNVEAIAHQASTFVSD
                     SSFLLGGDLSQTKKSLEEFLAVAKEYEGRITDWREQVGELIEKTPKWIDQASIVVTVF
                     LLWFGISQFGLILHGLNIRRGNDPFDVIRRRPVSLVERVREEDPNLLS"
BASE COUNT         3219 a         2607 c         2733 g         2881 t
ORIGIN      
        1 cgtttttcgg tggacataga actccccgcg atgatagatt aatcagatta tacggcaaac
       61 atggttcatt ttctacgaac tttcatcaca ttttgctgtt aaaaactcat tccctggtga
      121 tttgtatatc tgatggctgg caatagggca ctccccaatc caccataggg acattctcca
      181 ttgatttttc ggtcccttag ctacatgata ttagcaaagc actatcagat ttcaaaaagg
      241 agtgaacaca atggccacga atactcttca cgagaaagac ctgcaccggg tctttcatga
      301 aatccgccag gaaataggaa acgctcaatc ccgcgaggaa ttgaccgagc tttacaaacg
      361 aacgggttac atgatcacga tgacccatgc aaccccggtg gaagagaagc aggacacgga
      421 aatgaaggat cgccgcgaaa caacggagca cgaattcgcg acgaccgtgc gccttatcaa
      481 cagccgggca caggaacttg gcgtagaagc cgattacaac gagaactggg atggactagc
      541 cgctaatggc tacgaagcag agggcgaaaa cctgctggaa gccgaggaaa acgagcaatt
      601 tgatgaaggt gaagtaacca cccgataaat ggtagaaaac caaggcaaga gccagtcagg
      661 tagctgactg gctcttttag attccatgta aaagaatgcc aaatttgtct gacaactgac
      721 cttattaaat tgttaggtgg taggacttcc gtactatcct atgctatact taaatctcca
      781 cggaggaccc atgattgatg ttgctcaact cccgatcctg ctcaaagcca tcgattttat
      841 ctttgatgag gggcgaaaga tccttgaaga acgccgggaa cgaagaaaag cagaggcagc
      901 tcttcaggag acccagaacc cggaaccagc cgaagaggcg gctcccgttg aagagcagga
      961 gccagccgaa gtcaagcagg aattggtatc atccaaaata gatgaaatcg tgtggaaaga
     1021 ccatgaggcg gaagttaaac atctagtcaa gttgctggaa acctactccc acaactatca
     1081 tctggccaag gaacagtatg caaaatgggg aagcgcgctg gtacctccca ttattctgca
     1141 taatatgacc gaagcagaaa attcgatgct tgaaaccata cagaagctcg aaggtgtcct
     1201 ttcgaaggtc tacaaaaaag agatcaaccc cgttaaataa acacaaggtg cattatggac
     1261 aaggaagaaa ttaatcatct cgaaacgatg atttctttgc acaaaaaaaa cctctatatt
     1321 ctggaggaaa tgctggcgaa atatggggtg gatcagccgc tgcatctcgt caacagtgtc
     1381 acgatggaac gggaggcgat cgccagatac acgaaacaaa tcgagggact gacgttcaaa
     1441 ggccgtgaag ttgaaaaatc gactgcgctc gcaagcctgc caaggcgtcc ctattttgtg
     1501 gggcgggagg aggagatcaa gaccattttg cagtcgctgc agccaaacag ccggactttt
     1561 atcatcggaa ttgaggggat cggtggcgtg gggaaatcga cgcttgctac cgaagtcagt
     1621 cacttatgta ttgaaaatga gctttttgaa gccgtaatct gggtttcagc caaggaattc
     1681 acgctgacgc tgcatgggat cgagtcgatc acagccgagg tcaaatcgtt atcggatatt
     1741 ctgatcacga tcggaacaaa tttggggaat cccaccattg ggaatttgtc cattcaagac
     1801 cagatcaaac gagcctataa cctactttcc cggcagacca ctctgcttgt gctcgataac
     1861 ttcgaatcct tgtcgaaaaa cgagcagcgc gacatcctcg actttttgcg ccggtcaccc
     1921 atcacgctga aggtggtggt tacgtctcgg gaacgggtct ctgagggcca aattatccgc
     1981 ctgcagggat tgtcgtttga agaaagcgat gccctgctta aatgggatgc ccagcaaaaa
     2041 aacatccatc tcacaaagga tcagagcaaa cacctggtgg agttaacagg cgggctgcct
     2101 cttgccctgt tatgggtcca gggacaaatc gccgtgttgg ggtactcggt gacgcaggtg
     2161 ctggataaat taagtctcga tagcgatatt ccgatcttgc agtattgttt caaccatagt
     2221 tggaacttgt tgcgacatag caacgagaag gatttgttat tcatcctggc attgcagcca
     2281 gacgcagttt caagagcagc tctgaaagaa atttcagagc tggaagatgc agacgccttc
     2341 gaccgctcga tcagtgatct cttgcagtta tctctcatcg agcacgagca gaacagggat
     2401 tactttaacg ttctgccgtt aacgcgccga tttattcgca cccaatttgc cagcgaccgg
     2461 aaattcatca aacaggcaga actgaaaacc gctcaatact atgtgaagta tcttccacag
     2521 aagagcagtt tcaaagagtg gcgtggatat gatgatctat tgcgggatcg aaacaatatc
     2581 ctcagtgttg cccagtggtg ctacaagtct atacagaaaa aacagaatac caggaatcct
     2641 ctgacgaagc gggcgaaaaa catcgccgag attctggtac agattggtag acaatttggg
     2701 agtgtgttat ggcagcgggc ttactggtat gaccggatga cgctggccca tgcggcattg
     2761 aacgcagcaa aattgttgtc agactggaaa tctgtgagca cctttgcccg aaatatttcc
     2821 tggatctatt tttatcaggg tgattatctg cgtgccttac actggtctga ggaatccctt
     2881 tctgccacaa cgaagaccga agatgagctg ttgatcgcgg cggcaaagcg gtctttggga
     2941 acggttgaaa tgcgtctggg taattttgat cgctctcaaa aattattgga agaagtcatt
     3001 caaacgacag agcagttcac gaatgatgat tacggaatat atagtaaagg ctttgcccaa
     3061 tatggtctgg gtgacctcga aaatgagcgt gggaacacaa gcaaggctcg ggactggtat
     3121 caaaaagcat tggatacctg gcagaatcct gttcgcaacg atcccgttcg acatatcagt
     3181 tacgccttga acggcttggg ctttgttgcg ttaaaagaaa agaattacga agatgcaaag
     3241 cgccttttca tgaaaggcat ccaatcggca gaggagtttg ggcgctccga tgaactggcg
     3301 aaaggacagc ttgggctggc ctccgtctat tttgaaacag agtccgattt gaaaagtgcg
     3361 ctgacgttag tcaatgaggc aatcgagatt tttcagcagc atggtatgca gtacgaaatt
     3421 caaaagagtc gctctcttta tgatgaaatc ctgaaggtac aaacgcagat cacagcgcca
     3481 aggtaaccat ttaatggaaa atgaaaagga gattttcctg cgatccttta aggacctgga
     3541 aaatcaactt cagtccctgc ccatcgcgga cgctcaactt cagcaagtaa agggattcct
     3601 ttcaagcatt caaaagaatg ctgaaaccct ggctttgtat gatccgatta cacacgtctt
     3661 caatacgcgg gcgcgggaat ggatgatgcc aggcgtacca cttaagggaa tggcgaagat
     3721 tgatatctat gatttgcgac aggccaacaa agtttatggg atagctaccg tcgattcaga
     3781 gctgcataaa ctggcctata tgcttatgtc gatgttcact cttgagaacg gtgattctgt
     3841 ccatcggtcc cccggcagtg atgagtttcg gatattttcg acatcaaaaa cgccgaaaga
     3901 gctcaggttt ctgttgacta aaccctacct ggaccaggaa acagagtctc tgttgagctg
     3961 ggacttcggc accgggcatt cggaaacaga agcagagaat aacctgcaaa agcagcgaaa
     4021 aacctttcga cccatcgtca tccggcagac cattctggag agtcatgcag agattcccag
     4081 gcatattgag ggaaataaca gctatcaaag ttggaatgag tttaatatgc cttatgaaag
     4141 gcttgcagat acaatctatt cgttgaattt gccagttgct atagagcaac aaaccctcga
     4201 aaaacttcag accctcaaaa aaatcataga aaatttagtc acgaaagagg ccctcacaag
     4261 cacactcaat gcgcttggag ccaaatggta cctggatagc agtcttgttc aaagtgttgc
     4321 tttaacagac atgctcgata tgcatgaagg aaatgcgcgt tttgggagcc ttgcaattga
     4381 tcaggaactc agacgatttt cgaatatgct ctccgagcat tttccaaaag cagacggctt
     4441 tttgttattt cgatctgaac gcgcgggtga tgagtttaag atcacatcaa cgaaacttga
     4501 cacaacgcaa ttggaagaca agatccgcac ggtctggcag gaagacttgc accgcgggct
     4561 gctcgcctgg aattacggcg ttgggagaaa tgccggcgaa gcccatgtcc atctgtatcg
     4621 aaacagagtg aaggaaatag aaactatggc agcctcaaat tccaatggca aagcaacatt
     4681 cataattgtc agacccgaaa gtgacgatta tgcgggcttg tttaaacgtt ccgtagatgc
     4741 cgctcgcctg atcaatggga ctccgatcat tgatttacat ctgacgattc aggccatccg
     4801 aaatgttcag gattttgccg cgctgaaaga acgtctggag gaatactgtc tcaccctgca
     4861 accctttgaa gtgcgggtca gaaatatcgc cagaatgaat gttaataatc aacaaggcag
     4921 gctgtggctg ctggcagaaa aaacgccgat gctcgaagac atgtataaca acctggggaa
     4981 aatcgcagag gagatgggat gtgagagtta tccttataaa tctcaagatt ggctgccaca
     5041 catgaaaatt gtcgatttac ctgaacacac atcgactcag atcaaagacc ccaccttcgg
     5101 agtcggcaat ggagttacct tcactgtcaa tttctttgag tggacagtac aaaaagcata
     5161 tgatcactgg gaattgctgg atcagtttgt attcccatag taacaggtga atttctatgg
     5221 caaccatcct ccttgtggac gctaccccgt ccgaccgtcg atcgtatatg atgcttctgg
     5281 gtaattttgg tcatcgcttt cttgaagccg aaaatggcgc tcaggctctg gatatcgccc
     5341 gggctgaact tcctgacttg atcattacag acattctcat gccacatatt gacggattta
     5401 cgctggtgcg tcgtctgcgg tctgaaccac ttctgacagg cgtgcctgtc atttttcaca
     5461 cggctaatta tgatgtaacc gagatccaca ggctggcgcg ttccagtgga atacagcaca
     5521 ttctgagaaa acccgccgag ccgcaggaaa tactgcgagc ggtgaacgaa tcgctgaaac
     5581 accccaccca accttcgagg ctgccacaaa ctggtcaact tcaacgcgaa catctgcaat
     5641 tgctggcaga taaactttac caaaaggtcg ccgagcttga gaaagccaac gaaaaccttc
     5701 gcaacttttc actaacagac ggtctgactg gcttgaacaa ccgacgcggc ttcatgattc
     5761 tggctaccaa cctgttgaaa tttgcacgac gcgccaatta ttcgtcctgc cttctctata
     5821 tcgatttgga cagcttaaaa cagatcaata atacatttgg acatgcagcc ggggatctgg
     5881 cgatcaccca ttttgcacgt attttgaatg atacgtttcg cgagtccgac attattggcc
     5941 gcctgggcgg ggatgagttt gtgatcctga ttatcgacgc gtctgaaggg gatcttgcca
     6001 acatggaggc gcggctgcaa agcaatgtgg atgcccataa ccttcaagcg gatagtgagc
     6061 atgccctggc gttcagcctt ggaagcatcc gcgtagacgc gaactccacg atcaccatgg
     6121 aagcattaat gtctcaggca gatgaagcca tgtacaggca taaacagagc aggagacgtg
     6181 tcccacaata gctggaagga atattggttt tacagacact tccttaaggc ttgcagcaat
     6241 ttttgatagc catcgtcctc gaataaatcc acgcattgcc atcggcgcag gcgcccgggt
     6301 aaagcacatt tctccagccg taccggaata atgaatattt cctggctcgg gagcaggttt
     6361 gctttttcga gtgcgatttt cagctcttta tgtcgatagc cacccagctt gttaaattgc
     6421 cgcgaaagac aaataacgac cacgtcgctc tcaaggatcg ccttgcgaat ggcatgttcc
     6481 caatcctgcc cgggcaggat tttttctgcg tcaaaccagg tctctattcc aaattctttc
     6541 gcgacgcgct gatataactt ataaacttct gccttgtcgc tatgtgcata gcagaggaaa
     6601 accttaagtg ggggtttcga tttggacata ttttgcttaa atggaaacag gttcaatcgc
     6661 gggaaatcaa tcagcgggtc agttaaaaat tgtcaacata tacatgtttc aaatcattga
     6721 aaagaggcaa ttctgatcta cctaaattgc caggggcaat taagcctggc gaagtactat
     6781 accgggcttt attgaataac caatcgcgta acgtcttctc tgtcaaattc aggggttgca
     6841 aaacttcgtg caccgcacgc aggatcaatt ctttgtactg ggtaatatcg atggtggtgg
     6901 ggtcaggtgg aacaggcaaa tcccaggcat gtactccggg gcctcctcga atgtatataa
     6961 attttagctg ttgacccatc ttaatggttt taccctgtgc tttcagttgc ctggctgcga
     7021 ccgccagagg agataagacg ctgtaattat tcaattcacg actcaacgtt tgtgtgacag
     7081 tcaattctct taagggaaca attctatttt ttagttgatc aagcttttcc tgtgttatct
     7141 ctatgatttc aggcaaaagg cccatcagtt ttattggatc cgattctttt gcaagagtcc
     7201 ggatgatctg caattgcagg tcagtaacaa acttgcaggt atctgcgcgg cgaactgcaa
     7261 gacctctaat tttatgttcc ccattttctg caatcccgta gaatcgattc gcaactggaa
     7321 tcctgggatt ctgacgtgag ctgagaaaag caaaccagga ataaacgttt tcaaattcca
     7381 tggggaggcc agttgcctgc tcaatttcat tagcaagtgt ttgaaagtct tcctgagatg
     7441 catcagcgcg ggaaacaaac aggctatcga catatgcatg tagtacatcg aaacctctgg
     7501 cctcgacgag gaattttgca tctgtgatca ctttcctcga aaggtgagaa acaacttcat
     7561 gcgcgttcaa acgcccaaaa attgagttgg caaaacccag gcgtccgtaa caaacgacgg
     7621 tgagccactt tagcgcgttt gtgaccgctt tataacggtg atagatccca cgagtcttgg
     7681 gatcattttt atccatggat ttcaaccgtc gtttgagaga aagccgttta tcacgcatgg
     7741 atctgagggt ttgcgggatg agtcccggac gagaaaggat tttcacaccc agttcagaga
     7801 tctccaaccc ttcacccgct tcatggagac ggaccaccgt ctcaggcgag acgttgtact
     7861 tgatcatgat ggaagccatt ttcgaactaa aatccaaaat cgctacattt tggaaaatgc
     7921 ccggtgcggg ctggaagatc aaaccaccac gggcagcctg gtaaaactga tggaacgtct
     7981 tggggatctc gcccttttga tgttgataag ggacaagcgt gccgcgcttc agcgctgtaa
     8041 ggttttgcat tgaagcgatc gctgcgccgg gggaacgccg ggctacttcc tgcaggggaa
     8101 ggctgctcag ccgggtttgt tcaattgctc caatcaactg atactggttg tacgtcatac
     8161 agttcttaag atccacatgc catcgcccgc gcagatgaac ctgcggtgcc cgatagtgag
     8221 cctgcccgta gttgaagaaa ctgatctccc ggcgtctcag aacaggcatg gatacatcgc
     8281 ggttcgggtt gaatggaatc ctatattttt tggatacttc ccgcaaatac ggaaatagcc
     8341 acgcatcacc aaaatgtgtc cggatcacat ctggatcaaa tgaaaccagg atagcgttca
     8401 agaggtttat aagttcaggt ggtttttcca tggatattcg caagtaggat ttcctgaacg
     8461 ttgcaaccag atattgggga aaggcatgag atggatctgt atctggtttc aaggttagga
     8521 ttcgcaggcg cggtagtttt gggtccaggt catccagggt gtcaagcgca tgaatgtgta
     8581 cgatcgtatt gtcatcctcc gcgaccactt cacagcgcgc catcataaaa acatcatgag
     8641 cagccgcata ccgcactgtg agaggaatat caacatcata aaaaatcagg tctgaaaaaa
     8701 cccgataaac agccttaaac acggttttat agagatcagg acgagagacg gcaatcccca
     8761 tcacatcctg aggtcccgca aacaaatcct cctttgtcac acgctcaagc ctgaggttct
     8821 ctctggagta tccttttctc agaaagactc ccagctcgtg caaacgttga gcagagccag
     8881 aggcataaaa tatggtttca aaatcctgat ggaaggaata tcgttttccg tcctctccaa
     8941 ttaaccatag gacaacgcct ttcgtctggt gttcatacaa atcgaacaac caaccagtat
     9001 attttttcat gttttaagtt tgagttcttc gatttcacgc tttaattcca aaagctggct
     9061 gcgaagatcg ctgaactgat aattattatt ttcgtgttct tccacctgta caactacaag
     9121 ggctgcttcc agcggcaaca ggctggtggc attggaaata gaaacccggt tttgtaagat
     9181 gtattcacaa aatcttttta tgattctgcg atcactggga gaaagcatat cagcgagagg
     9241 cataagctca gcaatgatct tatgtgccag ctctgtagga gagaggttct ttgcggccat
     9301 ggacgtctgc taaaaaagca aattttgtat agcgtttgct gaagggatct cttttagagg
     9361 gatgatttca tttgcaatag aggcaagcac aggaaaaaac cgccggtatt ccatggtaga
     9421 taggcgtggt acaagtacaa cgacagaatt ggaaagtgcg agtcgctttg tatattgaca
     9481 gcaccgctcc aggacacgat ctcgcaggga gagttctaca tcagcgttgt aaaaatgatg
     9541 aaggaaattc aggatcaatg tgggtttatt ttgcatatca acttccatca gcagatccaa
     9601 cagttggagg caggtcatgg gtcgcatgag gctcgggtga ttcaacgctt cttcgacggc
     9661 cagggtatgc cagcggatgg aacggtaaag cgtatcgcgg tccggtaacc attcatctcc
     9721 agcaataagg ttgaagggac cgcgcaacgc aagttgagta attacctcaa cgatcacatt
     9781 gctttcctcg ttagaagttt caaccaaaag caaatgtcca aaccgatatg tgtttatcca
     9841 atgcgcagca cgaaaaggat gattacttct tcttatcggc agaaacccat gggtgtgtaa
     9901 gttatccatg tttgcaggat aatctattta cataaaataa aaaacaccca ttggatagta
     9961 tccaatgggt agtagccaca gggtagtagc aggtacagga gataagcgtt ttactgacct
    10021 attactaggg gggatatcgc tcgaaatcaa aattgtaaaa tttcaagcgt aattctgtct
    10081 tactccgaac atccatctta ttaaaaacat gttgaagata tgatttgacc gttcctacag
    10141 taacccccat atgaaaagcg atcttattgt tcttataccc catacacgtt aaataggtga
    10201 cattccgctc ccgtggtgaa agagataacc aacggtcata taaatcaagg aaatccttat
    10261 cagttgatcg gcggccacca gtcagatctc tagcttgggt attggaagag agattttcct
    10321 gggtggttgt gtctgttcgg ttaacaaggc gtaatgggtt agccaggacg tggaagtatc
    10381 tatttgtctg ctgtgagcct atccgtgaat tctttttctt tcgcggagta gacatagcag
    10441 aaattatatt agaacatata ttctattgtc aagtcattct agccgaaggt caggcgaaac
    10501 ctcggataac agtctcttag ccacggatta tgttgccatg gtcccacgcg acttttcccg
    10561 gagaataatt gatacatcta gggcaaaaaa gaggatctgc caacttctgt gccaaggtca
    10621 gatacgacac atggagatta agacaacaaa ttgggatctt cttccctcac ccgttccact
    10681 aagctaaccg gtctccttcg gatcacatcg aacgggtcat ttccgcggcg gatgttgagt
    10741 ccatgcagga tcaacccaaa ctgtgagatg ccaaaccaca acaggaaaac cgtcacaacg
    10801 atagaagcct gatcaatcca tttgggagtc ttttcgatta actctcctac ctgttcgcgc
    10861 caatcggtta ttcttccttc gtattccttc gccactgcta gaaactcttc caggcttttc
    10921 tttgtttggc tcaagtcgcc acccagaaga aaagatgaat cacttacgaa ggtcgaagcc
    10981 tgatgtgcaa tcgcttctac attagagatc tctgcgtcca ataagttggc agaagtgatc
    11041 aggtcagcca ggatttcatc aggcaggttt aagtcaaacg ataagaaaga gcttactcct
    11101 tccagcgcat tacgcagatt ttccagggca gcgcgtgccg caaggattct ctcacgagtg
    11161 gttttcagtt caggtaacaa tctgtcgtct agagtatctt ggatgttatc aaatatattt
    11221 tctgcactgg tagattgccc tgcaagtttt tccattgcat cttcggcagc atttaaaatt
    11281 cgtagcgcgc gttctaattc catttgagag ctttgcaggc tcgtttgtgc ttgtgacagt
    11341 tgggtatcga tttcagttaa tctattggtc gcttccagag tcaatggctc gttataaacc
    11401 caggcggcaa cgatgcccgc catactcaaa ataagaaata
//
LOCUS       DASTJP010000804         5415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_597780, whole genome shotgun sequence.
ACCESSION   DASTJP010000804 DASTJP010000000
VERSION     DASTJP010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597780"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFQ23_23520"
     CDS             complement(<1..559)
                     /locus_tag="VFQ23_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909637.1"
                     /translation="MKHLLLFITLATLSACAQQTAAKPLETPIPTTVSPTSIPISTLI
                     PVTVTPSPLPTEPIFPIITPNPIQVEKWNEYEDALATIFFKSYQPDDVVCEWVILGQT
                     DREVYVLAYCANIYSAGPSQASIPTVIHLGADGSVISAEIPGSGTGYGPDILRLFPPD
                     LHQIIFDHSVVPSPAQMDRLRWRRGH"
     gene            complement(733..1842)
                     /locus_tag="VFQ23_23525"
     CDS             complement(733..1842)
                     /locus_tag="VFQ23_23525"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9909638.1"
                     /translation="MNRLILVLLLATLACRSNTSTPSSIPTLIPTDFIPTAIAMTFEA
                     QSTSTSPAPTATIVPSTSTPTPTATQSSPTSTFTTSTDPYEQYGIDYLRQRSYGGGNF
                     EIVETTEVSDELTRTLIRYSSDDLTLYGFMNIPAREGPFPVIIMMHGLGDSGSFSELN
                     FRSDIVDTLTYSGYIVIHPIMRGYPPSDSGDNMFRVGMAVDMLNLIALIKTQSGQPGA
                     LEKAIPDQIGLLGQSMGGNVALRVLTVSSDVKAAVLYSSLSGDELKNSEQLYKVTVDP
                     QFQNELNILPSQLQSISPVYYYSNITAPIQLHHGVIDETVPVSWAEETCSLLTTAGKS
                     IECIYYENEDHVFDGDIKIQFLENAIPFFQTHLSP"
     gene            1885..3684
                     /gene="mutL"
                     /locus_tag="VFQ23_23530"
     CDS             1885..3684
                     /gene="mutL"
                     /locus_tag="VFQ23_23530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET9909639.1"
                     /translation="MPIRLLSSEVASQIAAGEVIERPASVVKELLENSLDAGARIVGI
                     NIEEAGKKLIEVADDGAGIPSDELELAVARHATSKLVRSDDLFSITTLGFRGEALASI
                     GSVSRMTITSRVANETEGAQLRVEGGKPGKLTKIGTTVGSTVRVEDLFYNVPARLKFL
                     KTDVTERRAIDSLVTRYALAYPDKRFKLVEGKNVTLQTSGDGDRRTILAALYGVDVAK
                     QMLEIISEEEGYRLTGLISPISLTRSNRKEITFFINGRWVHDASLSMALLQAYHTLLM
                     VGRYPLTALFLEMNPEEVDVNVHPAKAEVRFRSQDKVFSFIQRSVRKALLAYSPVPNV
                     APSLWGMTRTVQSEPLRNSVTDWAIAHDEKLQVSTDLLVEDRDQNLETQSPISGPEIH
                     QSTSTPSLVTTSRIPLLRLIGQIGATYLVAEGPDGLYLIDQHAAHERVLFEKLMTQHG
                     MKNIPSQTLLTPVVVTLPPHASKLLMNQLAELQKFGFDVEEFGPNIFQIRAMPALFMG
                     SDPAVALRALVEDFEEDETPLQNEVEAKLAARVCKRMAVKAGQTLSNEEQRALLLDLE
                     ACDSPRTCPHGRPTMIHLTVDMLERQFGRRGAR"
     gene            3674..4636
                     /locus_tag="VFQ23_23535"
     CDS             3674..4636
                     /locus_tag="VFQ23_23535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9909640.1"
                     /translation="MPDKHQTSRQLTVLFARYGIESFLESTSLLVALLEKSGELLSWN
                     PAFDSIKKTQPDKNYLKDFLSASRQDVFEELFTKAKRERARSQGNLEFVHESRNNSLA
                     CLFVPLPDERFLFIAEPVPANTLDAVAAELQTTKERLQRKETELKAVIAQANEVSHTD
                     SLTFLPNRRQIIGDLQREVMFSEQYGTPLAISLLDIDHFKNINDTHGHVTGDEVLREL
                     SDELRQHIRHPDTIGRYGGEEFLLILPHSTVIAATEQASRLCNYVRSLVIQAGGSDIT
                     LTISIGVAQFKIGREDWQTFLNRADKALYQAKNNGRDQWVVAEK"
     gene            4640..>5415
                     /locus_tag="VFQ23_23540"
     CDS             4640..>5415
                     /locus_tag="VFQ23_23540"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9909641.1"
                     /translation="MRALWLENNQISLRDVPPPSKPNEALVKIRKAGICSTDLELVKG
                     YYPYTGILGHEFVGEVVSLTPNSSPHRRGEGVNVGDRVVGEINAVCNVCEQCLNGRPT
                     HCENRTVLGIVNRDGTFAEYTTLPIANLHRVPDSVPDEMAVFTEPLAAALEIQQQIQI
                     SPTDRVLLVGAGRLGQLIAQTLALTGCDLRVVARHSRQQDLLKQRGIRTISEDEIERW
                     RWDIVVEATGSPGGFSLARQAIRPRGTLVLKSTYKGELNV"
BASE COUNT         1349 a         1298 c         1422 g         1346 t
ORIGIN      
        1 ggtgacctcg cctccagcgc aggcgatcca tttgcgctgg agacggcaca actgaatgat
       61 caaaaatgat ttgatgcaaa tctggaggaa acagtctgag aatatccgga ccatatccag
      121 ttcccgatcc gggaatttcc gcgcttatta ccgagccatc tgctccaaga tggattacag
      181 taggaattga tgcttgagag gggccagcag aatagatgtt tgcacaatac gccagtacgt
      241 aaacttcccg gtcagtttgt cctaagatga cccactcgca tacaacgtca tccggctggt
      301 aagatttgaa aaaaatggtt gccagtgcat cctcatattc attccacttc tccacttgaa
      361 tagggttagg tgtaataata ggaaaaatcg gctctgttgg caatggagat ggagtgacgg
      421 taacaggtat aagtgtcgat atgggaatag acgttgggga aacagttgtt ggaatgggtg
      481 tttccagtgg cttggcagca gtctgctgtg cacaggcgga cagtgttgcc aaagtgatga
      541 agagtagcaa gtgtttcata ttgggtgttt aatagatatt tgtttatatt gcaactgctt
      601 aggcttgttt taccacgttc cggcggactc aagtcctgcc tcagtacgtg aaagtccgct
      661 gaagcggact cgcgcaatca gcccgtaggg cttccatgaa ctgagcaggg gcttcagccc
      721 tgctctcgtt ctttatggcg aaagatgcgt ttggaaaaac gggattgcat tttcaagaaa
      781 ttgaatcttg atatctccat cgaagacgtg atcttcattt tcgtaataga tgcattcgat
      841 acttttgcca gccgtggtta ggagggagca ggtctcttct gcccagctca caggtacagt
      901 ttcatcgatg acgccatgat ggagctggat cggtgcggtg atatttgaat agtaatacac
      961 cggcgagata ctttgtagct gactcggcaa tatgttcaac tcgttctgaa attgtggatc
     1021 cactgtgact ttgtataact gctcggaatt tttcaattca tcgccgctga gggatgaata
     1081 aagcaccgcg gcttttacat ccgaacttac ggtcaaaacg cgtaaagcga cgttaccacc
     1141 catgctctga cccagcagcc ctatctggtc aggtatcgct ttttcgagcg cgcctggctg
     1201 tccactctgt gttttgatca gtgcgatcaa attgagcata tccactgcca tcccgacgcg
     1261 gaacatattg tcgccgctat ctgacggggg atatccacgc ataatgggat gaatgacgat
     1321 atagcccgaa tacgtcagtg tatctacgat atcacttcga aaattcaact cactgaatga
     1381 tcccgaatcg cctaagccgt gcatcatgat gatcactgga aacggacctt cgcgtgcagg
     1441 gatgttcata aatccataaa gagtcaggtc atcgctggaa tacctgatca gagttcgagt
     1501 gagttcatcc gacacttcgg tggtttcgac gatttcaaaa ttgcctccgc catacgaccg
     1561 ctgacgcaaa taatcgatcc catattgctc gtaaggatca gtcgaggttg tgaatgtaga
     1621 ggttggtgaa gattgcgttg cagtgggtgt cggcgtggat gtacttggca caattgttgc
     1681 agtcggtgct ggagaggtgg atgtcgattg tgcctcaaac gtcattgcga tagctgttgg
     1741 gatgaaatct gtaggaatca gtgtgggaat cgaagagggt gttgacgtat ttgatctgca
     1801 ggccagggtt gccagcagta gaaccagaat cagtcgattc attcgacctc cagagccgat
     1861 aattatagcg gttataatcg ccgcatgccc atccgtttgc tttcatctga agtcgcatca
     1921 caaattgccg caggtgaagt gatcgagcga cccgcctctg tggtgaagga actgttggaa
     1981 aattcactgg atgctggtgc gcgcatagtg ggaattaaca tcgaagaagc gggaaaaaaa
     2041 ctgatcgaag ttgcagatga tggggctggc attccttcgg atgaactgga actggcagtc
     2101 gcccgtcatg cgacctcaaa actggtccgc tccgatgacc ttttttccat cactaccctt
     2161 gggttccgcg gagaagccct ggcttcgatc ggctccgttt cgcggatgac catcacttca
     2221 cgtgtggcaa acgagacgga aggcgcgcag ttacgcgtcg agggcggaaa accaggcaaa
     2281 ctgacgaaaa taggtaccac agttgggtca accgtccgcg tggaggatct cttctataac
     2341 gttccggcgc ggctaaagtt cctgaagacc gatgtcacag aacgcagagc aattgactcc
     2401 cttgttacgc gctacgccct ggcatatcca gataaacgct ttaagttagt ggaagggaag
     2461 aatgtcacct tacaaacatc cggtgatggt gaccggcgta ccattcttgc tgcgctctat
     2521 ggcgtggatg tcgctaagca aatgctggag atcatatctg aggaagaagg ctaccggctc
     2581 accggactta tcagcccaat ctccctcacg cgttccaatc gcaaggagat cacctttttc
     2641 atcaatgggc gctgggtgca tgatgcgtct cttagcatgg ctctcctgca ggcgtatcat
     2701 acccttttga tggtggggcg ttacccgttg acagcgctgt tcctcgaaat gaatcctgaa
     2761 gaagtggatg taaacgtgca tcccgcaaaa gcagaagtgc gctttcggag tcaggataag
     2821 gttttcagtt ttattcaacg ctcggttcgt aaggctttac tggcgtactc ccctgtcccg
     2881 aatgtcgcgc cttcgttatg gggaatgact cgcacggtcc agtctgaacc gctgaggaac
     2941 tctgtcactg attgggcaat tgcgcatgat gagaaattgc aggtatctac tgatctattg
     3001 gtagaagata gagaccagaa cctcgagact cagtctccaa tttcaggtcc cgaaattcat
     3061 cagtcaacaa gtactccatc cctagttaca acttccagga ttcctcttct tcgcctcatt
     3121 ggtcagatcg gcgcaaccta tctcgtggct gaaggtccgg atggtctcta cctgatcgat
     3181 cagcatgccg cgcatgaacg cgtattgttc gaaaaactaa tgactcaaca tgggatgaaa
     3241 aacattccat ctcaaaccct gctcacgccc gtggttgtga ctcttcctcc tcacgcttcg
     3301 aaattgctga tgaaccaatt agctgaacta cagaagtttg gctttgacgt ggaagaattt
     3361 ggacccaaca tttttcagat ccgtgcgatg cctgcgttgt tcatggggag tgatcccgct
     3421 gtggccttac gtgccctggt ggaggatttt gaagaggacg aaaccccctt acagaatgaa
     3481 gtagaagcga aacttgcggc gcgcgtttgc aaacggatgg ctgtaaaagc ggggcagaca
     3541 ttatccaacg aagaacagcg agccttactg ctggatcttg aagcctgcga ttcaccgcgg
     3601 acctgcccgc atggcaggcc aacgatgatc cacctgacgg tggatatgct cgagcgtcaa
     3661 tttgggaggc gtggtgccag ataaacatca aacatccaga caattgactg tgttattcgc
     3721 tcgctatgga attgagtcct ttctcgagtc taccagcctg ctggtcgctc tattggaaaa
     3781 gagcggcgaa ttgctctcgt ggaatccagc gtttgattcg atcaagaaga cccagcctga
     3841 taaaaactat ctcaaggatt tcttatccgc ctcacgtcag gatgtctttg aggaactttt
     3901 tacaaaggcg aaacgcgaac gtgccaggtc acagggtaac cttgaatttg tccacgagag
     3961 tcgaaataac agtttagcgt gtcttttcgt gccgctgcct gatgaacgct ttttattcat
     4021 tgcagagcca gtcccggcga atactcttga cgcggtcgcc gcggaattgc aaaccaccaa
     4081 agagcgcctc cagcgtaaag aaacagagtt gaaggcggtc attgctcagg caaatgaagt
     4141 ttcccatacc gattctctga cattccttcc caaccggcgg cagatcatcg gagatttgca
     4201 gcgcgaggtg atgttctcgg agcaatacgg caccccgctg gcgatttcat tattagatat
     4261 cgatcatttt aaaaatatca acgataccca tggccacgtc actggagatg aagtccttcg
     4321 ggaactatca gatgaactgc gccagcacat ccgtcacccg gatacgatcg gtcgttatgg
     4381 cggtgaggag tttcttctta ttttgcctca cagcactgtg atcgctgcga cagaacaggc
     4441 cagccgtctc tgcaactatg ttcggtccct ggttattcag gcaggcggaa gtgatattac
     4501 tctcacgatt agcatcggag tagcgcagtt caaaataggc agagaagatt ggcagacctt
     4561 cctcaaccgc gctgataaag ctttatatca ggcaaagaac aacgggcgcg atcaatgggt
     4621 ggtggcagag aaataaatta tgcgagcgct ttggcttgaa aataatcaga tttctttacg
     4681 cgatgtccct ccaccgagca aaccgaatga ggcattggtc aaaattcgca aagcagggat
     4741 ttgcagcaca gaccttgaac tagtcaaagg ctattaccca tacacgggca ttctgggtca
     4801 tgagtttgtc ggtgaggtgg tctcccttac ccctaactcc tctccccata ggagaggaga
     4861 aggtgtgaac gtgggcgacc gcgtcgtggg tgagatcaac gcggtttgca acgtgtgtga
     4921 acaatgtttg aacggacgcc caacccactg tgagaatcgc accgtattgg ggattgtgaa
     4981 tcgtgacggc acatttgcag aatatacgac cttaccaatt gccaacttac atcgcgtgcc
     5041 ggattcagtt ccagatgaaa tggctgtttt cacggagccc cttgcggcgg cattagagat
     5101 acagcagcag attcaaatca gccctacaga tcgagtcttg ctggtgggcg cggggcggct
     5161 gggacaactc atcgctcaga ccctggcatt gacaggctgc gacctgcgcg tcgttgcacg
     5221 ccatagccgc cagcaggatc tattgaagca gcgcggcatc agaaccattt ctgaagatga
     5281 gatcgaacgg tggagatggg atatcgtcgt ggaagcgacc gggtcgccgg gcggtttttc
     5341 tttggcgcgg caggcgattc gtccgcgcgg gacgctggtg cttaaatcta cttataaagg
     5401 tgaactcaac gtcaa
//
LOCUS       DASTJP010000805         7667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_178564, whole genome shotgun sequence.
ACCESSION   DASTJP010000805 DASTJP010000000
VERSION     DASTJP010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178564"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..120)
                     /locus_tag="VFQ23_23545"
     tRNA            complement(48..120)
                     /locus_tag="VFQ23_23545"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Glu,seq:ttc)
     gene            complement(226..771)
                     /locus_tag="VFQ23_23550"
     CDS             complement(226..771)
                     /locus_tag="VFQ23_23550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909642.1"
                     /translation="MDSAPRTYRFLPADVYTSGYRVVGKIMVSNNGIVGILNDINKSY
                     LEIHDARLARIHMPTKLVDHFEVVRLVKAQIFAICAARREDLGPTNVVRGGYSQVSVL
                     PARLTTNVYELEGKLEVTGRFDFAVMIADRSRNFIPIYEAVLTAILMPSLKVETPAML
                     FNREKVDLIGLLGQRVKEEKQ"
     gene            complement(797..1096)
                     /locus_tag="VFQ23_23555"
     CDS             complement(797..1096)
                     /locus_tag="VFQ23_23555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HET9909643.1"
                     /translation="MTTPNTFPASNGSPCRECAAGVMQLRHITYFTWLGEELITVPNF
                     PAWICDMCGRREYDERAIAWLNMLLDPNAGKPTRRVKRAPRPRAAKRNLPPPPLD"
     gene            complement(1098..1706)
                     /gene="lepB"
                     /locus_tag="VFQ23_23560"
     CDS             complement(1098..1706)
                     /gene="lepB"
                     /locus_tag="VFQ23_23560"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9909644.1"
                     /translation="METYQPETQIQPEQSTTEQPTNWKRFVLDILETIVLAVVLYFGI
                     NAVSARVRVDGFSMNPTLQNGEYILVNKLAYKLGRPTRGDIVVFMFPMNPQEDLIKRV
                     IGLPGETVSVLNGVVSINGVPINEPYINAAPFYNGEWQIPEGQLFVLGDNRNDSRDSH
                     QWGLLPIENVIGRAVLIYWPPEQWQVIDHVKRASALMAAWTP"
     gene            complement(1820..2359)
                     /locus_tag="VFQ23_23565"
     CDS             complement(1820..2359)
                     /locus_tag="VFQ23_23565"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HET9909645.1"
                     /translation="MKRPISESYWVEPGRFLAGEYPGRYDAEQTRKRIDAFIEEGFNI
                     FIDLTRPNEISPYSHILLEQSQVYDLEVKQYRFPIGDFGLPTPELMKSILDTIDAELR
                     AGKKIYVHCWAGIGRTGTTVGCYLVRHGKSGDESLRQLGEWWRDVPKSQFHPRSPETN
                     DQMEFIRTWQEPKLTDSSA"
     gene            complement(2369..3376)
                     /locus_tag="VFQ23_23570"
     CDS             complement(2369..3376)
                     /locus_tag="VFQ23_23570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909646.1"
                     /translation="MEICYLLAFPDSDDGTSPPPEQIKGLKDAPYFQPIDIDLVTLGE
                     ETLIIEGYAVSVTRQRYDGVVQMVECHFDLLDPFAPSVLQERTKIQSALQSRFIPEEY
                     RRSGLFEEYTILLVDSAKPTPDKWLEKNALALANFIRSQREVLDEKEIGEILASRSRY
                     STQDLTLVDWEGAVIIAPNNDFQSDIVLLKIGNYQLLRYRMLDKRIEEMLDKINETFF
                     KNRRRPRATRGLIRQIAEHRLEVMLDFERAEQNLLLIGDWYTAKLYESIHNELYLKDW
                     KESVRSKLDNLENIVQTVKDNFLLSWENLMDRLQLLGWVILLVGYIYLYLLDAGWITF
                     P"
     gene            3513..5183
                     /locus_tag="VFQ23_23575"
     CDS             3513..5183
                     /locus_tag="VFQ23_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9909647.1"
                     /translation="MTIQEQYNKWKETTLKKSLGKFKERKERFETSSGIELPRVALPE
                     GGLDAERLDQQYLEKLGFPGEYPYTRGVQPTMFRSRLWTMRQYAGFGTAEETNKRYRY
                     LLAHGQTGLSVAFDLPTQIGYDADDPIAHGEVGKVGVSISSVHDMETLFKDIPLEQVS
                     TSMTMNAPAGVLLAMYIAVAKKQGADVKKLRGTIQNDILKEYIARGTYIYPPAPSMRL
                     ITDIFQFCASEVPAWNTISISGYHIREAGSTAAQEVAFTLSNAIAYVEAALRAGLSVD
                     DFAPQLSFFFNAHNNFLEEVAKFRAARRLWAKIMRERFKAQKPSSWQLRFHTQTAGST
                     LTAQQPENNVVRVTIQALSAVLGGTQSLHTNSMDEALWLPTEKAVRVALRTQQIIAYE
                     SGVADSVDPLAGSYLIEYLTDEIEKGAEEYITRIDDMGGALKAIEHGYMQNEIQNAAY
                     EAQQAIEKKEQIVVGVNQFQVEEEMTLERLRVDPAIEEAARERLKVLRQTRDQRQVEE
                     LLGKLKSAAAGTENLMPIIIESVENDITLGEICGALRAIWGEYVAEGF"
     gene            5261..>7667
                     /locus_tag="VFQ23_23580"
     CDS             5261..>7667
                     /locus_tag="VFQ23_23580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9909648.1"
                     /translation="MSHVSHKLKNSLEGALAGGGDPATSPLYVFGPFLKLIVFAGVAR
                     VTFGASVWLVVITIAVVSAMYRLVMQWVTDGSGGSGLSEEEFGGWAAKLNAAITFIEY
                     TLTFLVSMAAMVTFMADRFPILNQPVLAQTAFSYRTIVAILLSILTGWLVNRGPKMAA
                     RIFGPATAGVLLLLWGMVIASIIKYGFHLPDFNPAAFQLNYIHFTVGGYVRILAVMTG
                     VEVFANLVAAYDGTAAQKSSKAFYSLLIIMGTTAITMLVVGPVIYLVANPADVHVSVF
                     TQTMDHLLPKPLPFLGTMVGVAVLMSASAASAQGLQNLALGLRERRYLPPQLGEPNKY
                     EVAELPVWIEVGIVCFCFILLGTHEETYLALYAAGVFILLSMTGWAVTRRLMRAARAS
                     ASWRYAFLIPGTILAALLTSGATLIIFFERFWEGAWTYLLFLPSLYLVFTLFRRRYGD
                     PTPEMDYLGQLDAAQLAGFGFGQVAKLPIMPGQPISSKHVEVAWHPDYNDERTWMRQI
                     PDMKHIILLLDGSENAAQAIPISKVIATALNSQVVLLSAVKLREDTPAEKIQLAIRER
                     MRYLESTIHEFAAVGIKAEPRVEIGSTSEVSEKLIRQNGYGLVITATRGARGEHTWLR
                     GGLTHKLVENLETPVLLVQVSDKSNGMQQKFNRILVALDGSSYSERALPYARLLAKAL
                     NTKLTLLSVPAVPEAEEYRAPADIVRKIRQKAEANMQKFLAGTARLLRRDGLKVDLRV
                     TGSIPAHAILSVAEEERADMIILTSQGRGGYNALLLGSVADHVVQNTNALVFMVPIQE
                     NPNG"
BASE COUNT         1847 a         1893 c         2013 g         1914 t
ORIGIN      
        1 tcacttactc cattccacca aaatcggctg ctcactcttt agagatgtgc ccccacgggg
       61 attcgaaccc cggtcgtagc cttgaaaggg ctgcgtccta gtccactaga cgatgggggc
      121 ttatggcata gctgacacat gtccgctatg tgatttgcgg caactcgaag ctgcccaaat
      181 caagcgggcg gattttatca ttcccttatc taatggtcaa gcaatttatt gtttttcttc
      241 ttttacgcgt tgccccagca aaccgatcaa atcaactttt tcgcgattga agagcatcgc
      301 cggggtttcc actttaaggc tgggcatcag gatggcggtg agaacagctt catagattgg
      361 gatgaagttt cggctccgat ccgctatcat caccgcgaaa tcaaaacgac cggtcacttc
      421 gagcttacct tcaagttcat aaacgttcgt ggtcaagcgc gctggcaaca ccgagacctg
      481 actatagcct ccgcgcacaa cgttagttgg acccaaatcc tcacgccgcg cggcacagat
      541 ggcaaagatc tgagctttta ccaggcggac tacttcaaag tgatccacca gtttcgttgg
      601 catatggata cgtgccagcc gcgcatcatg gatctccaga taggatttgt taatgtcgtt
      661 caagataccg acaatgccat tgttggagac cattattttc ccgacaacgc gatatcccga
      721 ggtgtataca tctgcaggaa gaaatcggta tgttcggggt gcgctgtcca tgatcgctgc
      781 tttacatcag gcgagctcaa tcgagaggag ggggaggcag gttccgtttt gcagcgcgag
      841 gccggggggc acgtttgaca cggcgcgtcg gcttcccggc gtttggatcc agaagcatat
      901 ttagccaggc aatcgcgcgt tcatcatatt cgcgcctgcc gcacatgtca catatccatg
      961 cagggaaatt cgggacggtg atcagttcct cgcccagcca ggtgaaatac gtgatgtgac
     1021 gcaactgcat cacccctgcc gcacattcac gacatggtga gccattcgag gcagggaaag
     1081 tattcggagt ggtcatttta tggagtccag gcggccatta aagccgatgc tcgtttgacg
     1141 tgatcgatca cttgccactg ttctggaggc cagtatatca aaactgccct gccaataaca
     1201 ttttcaatgg gtaacaatcc ccattgatgt gaatcgcggg aatcattgcg attatctccg
     1261 agtacaaaca attgaccctc aggaatttgc cattctccgt tataaaatgg cgcggcattg
     1321 atataaggtt cattgatggg tacgccgttg atgctgacca caccgttcaa tacgctgact
     1381 gtttcacctg gcagaccaat cactctcttg atcaaatcct cttgtggatt catcggaaac
     1441 ataaacacga cgatgtcgcc gcgcgtgggt cgacctagtt tataagccag cttattgacc
     1501 agaatgtatt caccattctg cagggttggg ttcatgctaa agccatccac tcgcacgcga
     1561 gccgagacgg cgttgatacc aaaatataat acgaccgcca gaacaatcgt ttcgaggata
     1621 tccagcacaa aacgtttcca attggttggt tgctctgtag tagattgttc gggttgaatt
     1681 tgtgtttcag gttgataggt ttccaaactt tcgtgcctcc aaaaagatta tactgctttt
     1741 tgcaaggggc ttcctcaaac ttaaggctga tttaacgtaa ctgtatggtt tacgcaatgg
     1801 tattgaaaac caggaggttt caagcagaag agtctgttaa tttaggttcc tgccacgtgc
     1861 ggataaactc catttggtca ttggtctcag gggagcgtgg gtgaaattga ctcttcggca
     1921 catccctcca ccactcacct aattgacgca atgactcgtc accactctta ccatgcctca
     1981 caaggtaaca gcccaccgtt gtaccagtac gcccaatgcc agcccagcaa tgcacgtata
     2041 tttttttgcc tgcccgtaat tcggcatcga tcgtatctaa aatcgatttc attagctcag
     2101 gtgttggtaa accgaagtct ccaatgggga aacgatattg tttcacttca agatcataca
     2161 cttgtgattg ttccagcagg atgtgagagt aaggagatat ttcattgggt ctggtcaggt
     2221 caatgaatat gttgaatccc tcttcaatga atgcatcgat tcgtttgcgg gtttgttcgg
     2281 cgtcgtagcg acccgggtat tctccggcta gaaaacgacc tggctccacc cagtacgact
     2341 cagaaatagg gcgtttcata gcatcggctc acggaaaagt aatccagcct gcatccagta
     2401 agtataaata aatgtacccg acgagcaaaa tgacccagcc taacaattgc aggcggtcca
     2461 tgagattttc ccaggacaag aggaagttat ccttcacggt ttgcacgatg ttctccaggt
     2521 tatccagctt gcttctaaca ctttccttcc agtctttgag ataaagctca ttgtgaatcg
     2581 attcatataa ttttgcagta taccaatcgc cgatcagaag cagattttgt tcggcgcgtt
     2641 caaagtccag catgacctcg agcctgtgtt ctgcaatttg gcggatcagc cctcgggtcg
     2701 cgcgtggacg gcgtctgttc ttaaagaacg tttcattgat cttatccagc atctcttcga
     2761 tccgcttatc cagcatgcgg tagcgcaata attgataatt gccaatcttg agtaacacga
     2821 tatctgattg aaaatcgtta tttggcgcga tgatcactgc accttcccaa tccacaaggg
     2881 taagatcctg tgtggaatag cgcgaacggg aggcaaggat ttcaccgatc tctttttcgt
     2941 ccagcacttc acgctgcgag cggatgaagt tcgctagcgc gagcgcgttt ttttccagcc
     3001 atttatcagg agtaggttta gcgctatcaa cgagcagaat cgtgtattct tcgaaaagtc
     3061 cactgcgacg atattcttca gggatgaagc ggctctgcag cgcgctctgg attttagtcc
     3121 gctcctgaag gaccgacggc gcgaacggat caagcagatc gaagtggcac tccaccatct
     3181 gcacaactcc atcgtaccgc tgacgtgtga cagagaccgc atagccttcg atgatgagcg
     3241 tctcttcgcc gagggtgacg aggtcaatat caatgggctg gaagtagggc gcgtctttga
     3301 gtcccttgat ttgctcagga ggtggagatg tgccgtcatc actatctgga aatgcaagca
     3361 agtaacaaat ttccattcga taagtgtagc acggattaat tcctttggat ttatacttga
     3421 ggaaaactta accgccaagc acgcaaagaa cgcgaagatt tttaagagat tcttggcgac
     3481 cttagcgctc ttcgcggttc aaaggtctta tcatgactat ccaggaacaa tacaacaaat
     3541 ggaaagaaac cacgctcaag aaatcattgg gtaaattcaa agagcggaaa gaacgctttg
     3601 agacgtcttc agggattgaa ctcccgcgcg tcgcattgcc cgagggtggt ctcgacgcag
     3661 agcgtctcga ccagcagtat cttgaaaaac ttggtttccc cggcgaatat ccctacacac
     3721 gcggcgttca gccaacgatg tttcgctcgc gcttgtggac catgcgccag tatgcgggct
     3781 ttggcacagc cgaagagacc aacaaacgtt atcgttacct attagcacat ggccagaccg
     3841 gcttatcagt cgcattcgac ctgcccacac aaatcggcta cgacgcagat gacccgatcg
     3901 cgcacggcga agtggggaag gtgggtgttt cgatttcatc ggtgcatgat atggaaacat
     3961 tgttcaaaga tattccgttg gagcaggtct cgacatccat gacgatgaac gcacccgcgg
     4021 gggttttgct ggcaatgtac attgctgtcg caaagaaaca gggagcggat gtaaagaaac
     4081 tgcgcggcac cattcaaaat gatatcctca aagaatatat tgcccgtgga acgtatatct
     4141 atccacctgc gccatccatg cggctaatca ccgatatctt ccagttctgc gcctctgagg
     4201 tcccagcgtg gaatacgatc tcgatttcgg gatatcacat ccgtgaagcg ggctcgaccg
     4261 ctgcgcagga ggtggcgttt actttatcaa atgcaatcgc ctatgtggaa gccgcactaa
     4321 gagctggctt gagcgtggac gacttcgcgc cgcagctctc gttcttcttc aacgcgcaca
     4381 acaacttctt ggaagaagta gcgaagttcc gtgcagcgcg acgtctatgg gcgaagatta
     4441 tgcgcgagcg tttcaaagcg cagaaaccct cctcatggca attacgtttt cacacacaaa
     4501 ccgcgggctc gaccctcact gcgcagcagc ccgagaacaa tgtagttcgt gtgactatcc
     4561 aggcgctttc cgctgtcctc ggcgggacgc agagtctgca caccaattcg atggatgagg
     4621 cattatggtt accaactgag aaagctgtgc gtgtcgcgct ccgcacgcaa cagatcatcg
     4681 cctatgaatc aggtgttgcc gattccgttg acccgctggc aggatcgtat ctcatcgaat
     4741 atctcacgga cgagatcgaa aaaggcgccg aggaatacat tactagaatt gatgatatgg
     4801 gcggtgcgtt gaaagccatt gaacacggct atatgcagaa cgaaattcag aatgcagctt
     4861 atgaggcgca gcaggcgatt gaaaagaagg agcagatcgt cgtgggagtc aatcaattcc
     4921 aggtggaaga agagatgacg ctggagagat taagagttga ccccgcgatt gaagaggcgg
     4981 cacgcgaacg tttgaaggta ttgaggcaga cgagagacca gaggcaggtg gaagaattgc
     5041 ttggaaagtt gaaatctgct gctgcaggca cagagaactt aatgccaatt attattgagt
     5101 ccgtagaaaa cgacatcacc ctcggcgaga tttgcggagc gctgagagcg atatggggag
     5161 agtacgtagc ggaggggttt tgaggaatcg taaccaattc taattgccgc caggttcaag
     5221 tgggggtaga atattcccgt tttgcctgag gaccgcatct ttgtcgcatg tctcccataa
     5281 actgaagaat tcgcttgaag gggcccttgc aggtggcggc gatcccgcta cctcacctct
     5341 ttacgtcttt ggtccatttc tgaaattgat tgtctttgcc ggggttgctc gcgttacatt
     5401 tggcgcgtct gtttggcttg tagtgatcac gatcgcggtc gtatctgcca tgtatcgtct
     5461 ggtgatgcag tgggtcaccg atggaagtgg tggcagcggg ctgagcgagg aagaattcgg
     5521 tggctgggct gcaaagctca atgccgccat cacgttcatt gaatacactc tgaccttcct
     5581 ggtgagtatg gcggctatgg tcacgtttat ggccgatcgg tttcccattc tcaaccaacc
     5641 agtattggca cagaccgcct tctcctatcg gacgatcgtt gccatcctgc tgagtattct
     5701 aacgggttgg ctggtgaacc gagggccgaa aatggccgcg cggatttttg gccctgccac
     5761 tgcaggcgtt cttttgcttc tctggggcat ggtcattgct tcaatcatta agtacggatt
     5821 tcacttaccg gatttcaatc cagccgcatt tcagcttaac tacatccatt ttaccgtggg
     5881 cggatatgtc cgcatcctgg cggtgatgac aggcgtcgaa gtttttgcca acctggttgc
     5941 agcgtacgat ggtacagccg cgcagaaaag cagcaaggct ttctacagct tgttgatcat
     6001 catgggcacg acagctatca cgatgctggt tgtcggtcct gtgatttatc tcgttgcaaa
     6061 cccggcagat gtgcacgtct cggttttcac tcagacgatg gaccacctgc tgccgaaacc
     6121 tctcccgttt ttgggaacga tggtcggtgt ggcggtgttg atgtctgcct cagcggcgag
     6181 cgcgcaaggg ctgcagaatc tggcgctcgg tctgagggag aggcgttatc ttcctccaca
     6241 acttggagaa cccaataaat acgaggtagc cgaactcccg gtctggatcg aagtgggaat
     6301 cgtctgtttt tgctttattc ttttgggtac tcatgaagaa acctatctgg cactgtatgc
     6361 tgcaggggtg tttattctgc taagcatgac gggctgggct gtgaccaggc gtctgatgcg
     6421 ggctgcccgt gcctctgcct catggcgata cgccttccta attcctggca caatcctggc
     6481 ggccttattg acaagcggtg caacgctgat tatttttttc gaacggtttt gggagggtgc
     6541 gtggacctac ctgcttttcc ttccttcttt gtacctggtt ttcacactgt ttcgcagacg
     6601 ttatggagac ccaacgcctg agatggatta tctggggcag ttggatgcag cacagctcgc
     6661 cggattcggt tttgggcagg tagccaagtt gccaattatg ccagggcagc caatttcctc
     6721 aaagcatgtg gaggtggcct ggcatcctga ctacaatgat gagcgcacct ggatgagaca
     6781 gattcccgat atgaaacata tcatcctttt gctcgatggt tcggagaatg ctgcgcaagc
     6841 tatacccatt tcgaaagtga tcgcgacggc gttgaactca caggttgtgc tgttatccgc
     6901 tgtcaaattg cgcgaggaca cacccgcgga gaaaattcaa ctcgccattc gtgaacgaat
     6961 gcgatatctg gagtccacta tacatgagtt tgctgcggtc ggcataaagg ctgagccgag
     7021 agtagaaatc ggatccacca gtgaagtgtc agagaagctg attcgtcaaa atggctatgg
     7081 cttggtcatt acggcgacgc gaggtgccag gggcgaacac acctggctca gaggcggact
     7141 gacgcataaa ctggtcgaga acctggagac gccggttctg cttgtacaag tgagcgacaa
     7201 aagcaatggc atgcagcaaa aattcaaccg aattcttgtg gcgctggacg gttccagcta
     7261 ctcggaacgc gctctacctt atgcccgctt gttggcaaag gcgctgaata cgaaactcac
     7321 gctgctgtcg gtgccggctg ttccagaggc ggaggaatat cgcgcaccag ccgacattgt
     7381 gagaaagatc cgccaaaaag cagaggcgaa tatgcaaaaa ttcctggcgg gtactgcacg
     7441 cctcttgcgc cgtgatggtc taaaggttga cctaagggtg accggttcga tccccgcgca
     7501 tgccattctc tcggtggcag aggaggaacg cgcggatatg ataattctta cgtcgcaggg
     7561 gagaggcggt tacaacgcct tattgctagg tagcgtcgcg gaccatgtgg tgcagaacac
     7621 gaacgctttg gtgttcatgg ttcctattca agaaaatccc aatgggg
//
LOCUS       DASTJP010000806        12338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1447040, whole genome shotgun sequence.
ACCESSION   DASTJP010000806 DASTJP010000000
VERSION     DASTJP010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12338
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447040"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1115
                     /locus_tag="VFQ23_23585"
     CDS             33..1115
                     /locus_tag="VFQ23_23585"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HET9909649.1"
                     /translation="MTKKISRRDALKFGALGFLGGLAACSPVARATETLLPTYAPFPT
                     STPLPPAATVPGTTPTAGPVITTLINDIAAYATRYLESLDEAQRTKSTYAFTDPELTR
                     WHWTTPGNFPRNGLPLREMGTSQRDAAFALLQASQSAYGLQKSLDIISLQNDLGNDPE
                     LYYVTVFGTPGVEPWGWRWEGHHLSRRFNVINGKLSVTPFFLGAWPTVNNAGLKAMER
                     EEWAARELLTSMDDSQRASAIFQQDTLGRHVTQNDAYVAPLENIGIAASNLNANQQAL
                     ITEIIHKYLDTLPDLVAAPHLDRLQAAGIEKITFAWAGPLEAQRPHYYRLQGPTFLME
                     HDNSRNRGTHIHSVWRDFAEDFGQTF"
     gene            1229..1858
                     /locus_tag="VFQ23_23590"
     CDS             1229..1858
                     /locus_tag="VFQ23_23590"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET9909650.1"
                     /translation="MVRVLLRRNLIVHILAALPLLLTAYEYVTNTLPIVLDRHLVIRF
                     GAVGIAFLVAAFSITPISILFGKTNLKPIRRPLGVYGFCYIALHRLAYAWLSNGFDWN
                     LILRDIGERRAMSVGLLAFALLIPLALTSTNGWQKRLGRRWKVLHRLVYFAVPLSILH
                     YFWLERDIRDWVLVYFALVVFLFIVRLPAIRQAFLRNRQHPQVHTIDAD"
     gene            2114..3094
                     /locus_tag="VFQ23_23595"
     CDS             2114..3094
                     /locus_tag="VFQ23_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET9909651.1"
                     /translation="MESIWLWVGFNVFVLAMLALDLGVFHRKSHAVSGKEALTWSLVW
                     ISLSLAFNAVIYFFWDRMVPGSTYTNNEAALAFFTGYLIEKSLSVDNIFVFILIFSFF
                     AVPAAYQHRVLFWGILGALIMRGTLIALGAALLKEFHWIIYVFGAFLIFTGIRMWRHQ
                     DEEVQPDKNPVVKFFKRFMPVTDNFEKDKFFVRRAGKLMATPLFLILLIVESTDLVFA
                     VDSIPAIFAVTREPFIVYTSNVFAILGLRALYFLLADVMDKFQYLKLGLSAVLTFIGV
                     KMVITDLYHIPVGISLAVVAGILTISVLASLWKAKSSPKELTPKELPAIE"
     gene            3233..3697
                     /locus_tag="VFQ23_23600"
     CDS             3233..3697
                     /locus_tag="VFQ23_23600"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9909652.1"
                     /translation="MTNSPQFSQAIRSWMDIFMHRSMRGWGLFAKSTGLSMPQFSVMM
                     QLHHRGNCAIGDISERFDITNAAASQLVDKLVQSGFINREEDPQDRRAKMLNLTDKGR
                     DLIQRGMEERYRWVDQLSEKLTTAERAKVTEALNLMTQAAKELEAELAQEAA"
     gene            3779..5767
                     /locus_tag="VFQ23_23605"
     CDS             3779..5767
                     /locus_tag="VFQ23_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909653.1"
                     /translation="MQATSNRPQSRTRTPGQRPSFKGIARAVRYLTRYGRQAALPYIF
                     LIIATLSQLAVPRMVRNVIDAVTSGYVADQILNALDQIPANLVGAALPQILEATGKDA
                     SLTLDQLKVQLDADVTNAPQALLYALIAIIVFATLRGLFSFLQAFWAEKNSQAVAYDL
                     RNDLYAKIQRLSFSYHDKNQTGQLMIRATDDVEKVRLFIGQGLLQLVGAVILLTGTII
                     ILFTSNVTLAWTAMPILPVAIVIFIIFASLAQPMFAKVQQKLSHLNTVLQENLAGIKV
                     IKAFTREKEQQGKFQHAADETMNQQIAVSRLFTFMFPFVFLVANLGQAAILYVGGKQI
                     IAGTLGLGAWQEFSLYLMYLFFPVMMFGMIITQMGQAGASAERIFEILDAKSDIVDKP
                     DAIKLPDVKGKVKFENVTFRYFGGGEPVLNNVSFEANPGETIALLGATGSGKTTIINL
                     LPRFYDPSEGKITIDGHDLRDVTLDSLRSQIGIVLQETTLFSGTIRENIAFGKPDATQ
                     AEIESAARAAAAHDFIMTFPDGYNSHVGERGQTLSGGQKQRVAIARALLLNPRILILD
                     DSTSSVDLNTEAHIQKALDSLMKGRTSFVIAQRISTVINADKILVLDKGKIVEQGKHA
                     DLMEESAIYAEIYNSQLLHTSEAPTSGETLEAEKEGAL"
     gene            5764..7764
                     /locus_tag="VFQ23_23610"
     CDS             5764..7764
                     /locus_tag="VFQ23_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909654.1"
                     /translation="MMRFGEMLNKETIKPRNLSETLARLGQYFVQYWYMILLAVLFVV
                     VSTWTQVTTPELTGQATDCFLVPAGASALEGFGSFGNQAGSSTQASTCYLATDDPSTL
                     SFTRQIVYNAYTLGGFQTPDPLSMTSDQRIEGLTRLIIVMVALFVLSSVLTGATFFTM
                     AWTGQHVLRVLRVQVFEQLHKLSLSYYSEHEAGDLMSRITNDTTAIEQAFSFALVNVF
                     SGILLIVWVSYNMLTTNLPFALLSLAVAPIMFLATYYFSEQARKAFRVSREEIGNVNA
                     ELQESIAAVREVQAFNRADENIEQFKEVNAANRDANVRAVAYTSALAPTLEALSYVAL
                     AIVTGVGGWYLLKGQTLMGTTVTLGIVVTFLGYVQRFNQPIQQIAVLWTNIQNAIAGA
                     ERIFTILDEKPAVNNKPAAKEMPEIKGLVEFENATHSYEDGVPVLKDVSFTAQPGQTI
                     AIVGPTGAGKTTIINLIPRFYDVTSGAVKIDGVDVRDVSMKSLREQIGIVLQDTFLFS
                     QSVMENVRFGRPDATDDEVKAAIRLANADSFIERLPEKYETVLGERGSGLSQGQRQLL
                     SIARAALADPRILILDEATSSVDTRTERLIQTAFDELLKGRTAFVIAHRLSTIRNADV
                     ILVLKDGQIIERGRHDELLAKEGFYYDLYMSQFKKQEEMEVE"
     gene            7857..9221
                     /locus_tag="VFQ23_23615"
     CDS             7857..9221
                     /locus_tag="VFQ23_23615"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET9909655.1"
                     /translation="MTLSPALTYLEEHYDRFQAELVELLRIPSISHDPAHKEDMDEAA
                     NWLANKLRSMGINNVEILPTAGHSVVYGESLKGGDSTPTVLIYGHYDVQSSEPLKDWN
                     SEPFEPTIRDGNLYARGATDMKGQTLAAVNAVEAILKSENLPINIKFLIEGEEEIGSR
                     HLNEFLVAHREKLACDFSLNTDAGGMPDANTPSICYSLRGGVAFVLQIFGPSQDLHSG
                     EYGGVVQNPIHVLSKLIAGLHDENGHVTLPGFYDKVRNLDDEERAEMARLPFDEEFLL
                     KHSGAPALWGEPEYIPAERIGARPTVEVVQFEAGQPKSAIPAKATARFSFRLVPDQDP
                     NEIHQQFKHYLETHAPATVNWDLEMIIGNPAVITDRKSREVLAMKDALQTAFGTPPIF
                     QRSGGGIGAVLMFKQTLGVDSVLTGFSLFDDNLHGPNEKLHLPTWKKGMAALVHFFHN
                     LVNR"
     gene            complement(9282..9413)
                     /locus_tag="VFQ23_23620"
     CDS             complement(9282..9413)
                     /locus_tag="VFQ23_23620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909656.1"
                     /translation="MNTCLVELMIQGMTMTPLLRGLNVLSSIPTQPDPHLEELKKET"
     gene            9518..10156
                     /locus_tag="VFQ23_23625"
     CDS             9518..10156
                     /locus_tag="VFQ23_23625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET9909657.1"
                     /translation="MYVAIEGVIGVGKTTLARLLQSTFNAEILLEVFEENPFLSDFYA
                     DRNRYAFQTQIFFLLSRYHQQRRTVLDMLSTGTNLFADYTFAKDALFARINLKGDELD
                     MYYKVHEALAEKIQKPDLLVYLQATTDTLMQRIAQRDRSYERQMERSYINELNLAYDE
                     FFSKPFDHTPVLTIDSNNLNVVQNPEDLKLIENRIRQSLSLPPFQQTLPLPH"
     gene            10177..11205
                     /locus_tag="VFQ23_23630"
     CDS             10177..11205
                     /locus_tag="VFQ23_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909658.1"
                     /translation="MRVTRESLIRIAKETAQQRAFNDRDIIAAYLSGSLASDEKDPLL
                     GGTADIDIIFVHVDDPLIRREFVKLTPDFHVDISHRAKAEFKKPRELRVDPWLGWEMY
                     DPMLLFEREKFFEFIQAGLRAGFEFNAPAPALHRSRLLLAKARQLWRELLAIEDVVTP
                     GDVLQYMDSLYHAVNAVAELSGPPLQERRLLLDFAQRAETAQRPDMDAALIGLLGGSA
                     LDSASISEWLPEWKLAFESAMQDSDVDPRIHSSRINYYEKALREILANDNPQAALWPL
                     WQTWTLAAKVLPNFAQDPWRGVCGQLGFTSQGMEEHVTGLDHFLDEVEVLLDELTAQY
                     GLETSTTL"
     gene            11372..11950
                     /locus_tag="VFQ23_23635"
     CDS             11372..11950
                     /locus_tag="VFQ23_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9909659.1"
                     /translation="MTRDEALALVREYVKNEGLVRHMLSVEAAMRFYAEKFGEDLETW
                     GLIGLLHDFDWEIHPTLEQHPQDGAPILRERGVPEEIIQDILSHADHLNLPRDTIRRK
                     AICACDEITGLITAVALVRPSRSLYDLEASSVKKKWKDKAFAAGTSRTEMGHAAQEFG
                     IELWEHTGNVIQAMRRIAPELGLVGNIQQPSS"
     gene            12082..>12338
                     /locus_tag="VFQ23_23640"
     CDS             12082..>12338
                     /locus_tag="VFQ23_23640"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HET9909660.1"
                     /translation="MSVTDEIKARINIVDLVSEAGVKLRHAGKNYTGFCPFHENKKTP
                     AFVVWPESGTWRCFGQCNEGGDVFKFVMKRENLDFKDALNTL"
BASE COUNT         3023 a         3122 c         2975 g         3218 t
ORIGIN      
        1 acgatctgta gttttccttt tggaggtatc ccatgacgaa aaaaatctcc cgcagagatg
       61 cgctaaaatt cggcgcactg ggtttcctcg gtggccttgc agcctgctca cccgttgctc
      121 gtgcaactga gacgttactc cccacctatg ccccatttcc aaccagtaca cctctcccac
      181 ctgctgcaac tgtgcccggc acaacgccca cggctggtcc tgtcatcacc acattgatca
      241 atgacattgc ggcttatgcc acgcgttatc tcgaatcgct agatgaagct caacgaacaa
      301 aatccaccta cgcgtttacc gaccccgaac taacacgttg gcactggaca accccgggta
      361 acttcccacg caacggtttg ccattgcgcg agatgggaac ctctcaacgt gatgcggcat
      421 ttgccttatt acaggcaagt caatcggcct atggcttgca aaagtcgctg gatatcatct
      481 ctctacagaa tgatcttggc aatgatcccg agctttacta tgtgaccgtt ttcggcacac
      541 cgggcgtgga accctggggc tggagatggg aaggccatca tctttcacgc cgcttcaatg
      601 tgatcaatgg aaaactttca gttaccccgt tctttcttgg tgcttggcca acggtcaaca
      661 atgccgggct caaggcgatg gaacgcgaag aatgggcggc gcgcgagctt ctcacttcga
      721 tggacgatag tcaacgcgcc agtgctattt tccagcagga cacgcttggc cgacatgtca
      781 cacaaaatga cgcctatgta gctcccttgg aaaatattgg aattgctgca tcgaatctca
      841 atgcgaatca acaagcactg atcacggaaa ttatccataa atatctcgat accctgcctg
      901 atctggttgc ggctccccat ctggaccgtt tacaagccgc cggtattgaa aaaattacct
      961 tcgcctgggc tggcccgctc gaagcgcagc gtccacacta ttaccggctt cagggtccca
     1021 ccttcttaat ggaacacgac aattcgcgaa atcgcggtac ccatattcat agcgtgtggc
     1081 gcgatttcgc cgaggacttt gggcagacgt tctagaaaat caagagaccc gaatggtttc
     1141 ctttgcatgt gaatccatac ttcaagcgta atttttatgc acaggttcct tccggtttat
     1201 cgaaaaccat tagagtctga attttgacat ggtgcgagtt ttacttcggc gaaatctaat
     1261 tgtacacatc ctggctgcat tgccattgct gctgaccgct tatgaatatg tcaccaatac
     1321 gctgccgatc gtcctggacc gtcatcttgt gatccgcttt ggcgcggtag gaatcgcttt
     1381 tctcgtcgca gcattttcga ttacaccgat cagcattttg tttggaaaaa cgaatctaaa
     1441 gcccattcgc cgtccgttgg gtgtatatgg gttttgttat atcgcattgc atcggctggc
     1501 ttatgcctgg ctgagcaacg ggttcgactg gaatctgatc ctgcgcgata tcggtgaacg
     1561 tcgcgccatg agtgtcgggc tcctcgcatt tgcactcctg atccctctgg cgttaacctc
     1621 tacgaacggc tggcaaaaac gattgggccg gcgctggaaa gtcctgcatc gacttgtgta
     1681 tttcgccgtg ccgctcagca ttttgcatta cttctggctg gaacgtgata ttagggattg
     1741 ggttcttgta tatttcgccc tcgtagtatt tctgtttatc gtccgattac cggccatccg
     1801 gcaggctttt ctgcgcaatc gccagcaccc ccaggtacat acaattgatg cggactgaga
     1861 ctcttataag aattgtacaa aaagctaaca aaattctaat ttgacaggct tagtccaatt
     1921 atgctaagat ttgtcaactt aagtagggag tagccatggc gccgcgggcg ccatcgatca
     1981 gtcgtcaata cggattatta tcccggctga ccgtacgtgt gccgacacaa ctggcgagac
     2041 taccacgatt tggtcgtggg gtctcgtttt tttattcccc gaccagacac aaaacaatat
     2101 aaggagtttt gttatggaat cgatttggtt gtgggtgggc tttaacgtat ttgtgttggc
     2161 aatgctggcg ctggacctgg gggtctttca tcgaaagtct catgcggttt ctggcaaaga
     2221 agcacttacc tggagcctag tctggatctc gctgtcactg gccttcaacg ccgtgatcta
     2281 tttcttctgg gatcgaatgg tacccgggag cacatatacc aataacgaag ccgcacttgc
     2341 atttttcaca ggctacctga tcgagaaatc tcttagcgta gataacatct tcgtcttcat
     2401 tttgatcttc tcgttctttg ctgttccggc tgcgtatcag catcgcgtcc ttttctgggg
     2461 tattctcggc gcattaatca tgcgtgggac gcttattgcc ctgggtgcag ctctgcttaa
     2521 agagttccac tggattattt acgtctttgg tgcgttcttg attttcacag gaattcgaat
     2581 gtggcgtcac caggatgaag aagtgcagcc ggacaagaat cccgtcgtta aatttttcaa
     2641 aaggtttatg cccgttaccg ataactttga aaaggacaaa ttcttcgttc gccgcgcggg
     2701 gaaattaatg gcaacgccgc tgttcctgat cctgctgatc gtcgaaagca cagacctggt
     2761 gtttgcggtg gattcgatcc ccgcgatatt tgcagtaaca cgggagccct tcattgttta
     2821 tacatcgaat gtctttgcca ttctgggctt gcgggcgctg tacttcctgt tggcagatgt
     2881 catggataag ttccaatacc tgaagcttgg gttatctgcc gtgctgacct ttatcggtgt
     2941 caaaatggtg atcacagacc tataccatat ccctgttggc atttcacttg ccgttgtggc
     3001 cggcatcctg accatatccg ttttggcttc cctatggaaa gcaaagagct ctccaaaaga
     3061 actgacccca aaggaactac ccgcaatcga ataacgcttt catactttag caaaaatgag
     3121 tgtaggtttc tacactcatt ttttatttcc gcaattctca atttctgccc cttgacaaaa
     3181 acttaaccca ggtataaagt taagcacgct taactaaaaa gaaggcttaa ctatgaccaa
     3241 ctcccctcag ttctctcaag ccatccgttc ctggatggat atctttatgc accgttcgat
     3301 gcgcggctgg ggactctttg caaagtccac cgggctttcc atgccgcagt tcagtgtgat
     3361 gatgcagcta catcaccgag ggaactgtgc cattggcgat atcagcgagc gctttgacat
     3421 caccaatgct gccgccagcc aactggtgga taaactcgtt caaagtggat tcatcaatcg
     3481 cgaagaagat ccgcaggacc ggcgcgccaa aatgttaaat cttaccgata agggcagaga
     3541 cctgatccag cgcggtatgg aagaacgcta tcgctgggtg gatcaactat ctgaaaagct
     3601 aactacagca gagcgcgcaa aagtcacaga ggccctgaat cttatgactc aagcggcgaa
     3661 agaactggaa gcagaattag cccaggaagc tgcctgacct cctgacagat ttcatactca
     3721 attcataagt aagccgtaaa ctcgatgcga tttaattgtt tatcttcaaa ggaaacctat
     3781 gcaagctaca tccaacagac ctcaatcccg aactcgtact ccgggacaac gaccctcctt
     3841 caaaggcatt gctcgtgcag tccgctatct gacgcggtat ggacggcagg ctgccttacc
     3901 atacatcttt ttgatcattg ctacgctctc acaacttgct gtgcctcgta tggtgcgcaa
     3961 tgtcattgac gctgtgacca gtggttatgt tgcagaccag attttgaatg cgctcgatca
     4021 aattccggcc aacctggttg gtgctgcgct gccgcagatt ctggaagcca caggcaagga
     4081 tgcatcctta acgctggatc agctcaaagt ccagttggat gcggatgtca ccaacgcccc
     4141 gcaggcgctg ctctacgcgt tgattgctat cattgtcttc gcgacgttac gtggtctctt
     4201 ttcgttcctg caggcgttct gggcagagaa aaactcgcag gccgttgcct atgatttgcg
     4261 taacgatttg tatgcaaaga tccagcgact ttcgttttct taccacgata aaaatcagac
     4321 cgggcagttg atgatccgcg cgacggatga tgttgaaaaa gtccgtttgt tcatcgggca
     4381 gggactttta cagcttgtgg gcgcggtgat cctattgacg ggtaccatca tcattttatt
     4441 cacctccaat gtcacgctcg catggacggc gatgcccatc ctgccggtgg cgatcgtgat
     4501 cttcatcatc tttgccagcc ttgctcaacc gatgtttgca aaagtgcagc agaagttatc
     4561 tcatttgaac actgtcttgc aggagaacct ggcgggtatc aaagtgatca aggctttcac
     4621 gcgtgagaag gaacaacagg gcaaattcca acatgctgcc gatgagacca tgaaccagca
     4681 aattgcagtc tcacgtttat tcacgttcat gttcccattt gtgttcctgg tggcaaatct
     4741 cggacaggct gccattcttt acgttggtgg caaacagatc attgctggca cattggggct
     4801 tggcgcctgg caggagtttt ctctttatct gatgtatctg ttcttcccag tcatgatgtt
     4861 cggtatgatc atcacgcaga tgggacaggc aggcgcgtca gcggaacgca tctttgaaat
     4921 tctggatgcg aaatcagata ttgtagataa gcctgatgca atcaagctcc ccgatgtgaa
     4981 gggaaaagtc aaattcgaga acgtaacttt ccgctatttc ggaggcggcg aacctgtgct
     5041 caataatgtt tcgttcgagg caaatccggg cgaaacgatc gcgttgctgg gtgcaaccgg
     5101 ctcaggcaaa acgacgatca tcaacttgtt gccgcgcttc tatgacccaa gcgaaggcaa
     5161 aatcaccatc gatggccatg atctgcgtga tgtgactctc gattcacttc gttcacaaat
     5221 tggtattgtc ttgcaggaaa cgacgctctt tagcggaacc attcgcgaga acatcgcatt
     5281 tggcaaaccg gatgccacgc aggcagagat cgaatccgct gccagggctg ctgccgcaca
     5341 tgatttcatc atgaccttcc ccgatggata caactcacac gtcggtgaac gcggtcagac
     5401 gctttcgggc gggcaaaaac aacgcgttgc cattgcccgc gccttactcc tcaacccgcg
     5461 cattctcatc ctcgatgact caacctccag cgttgacctc aacaccgagg cacacattca
     5521 aaaagcactt gattcattga tgaaaggacg cacatctttt gtgattgcgc agcgtatcag
     5581 cacagtaatc aacgccgata agatcctcgt ccttgataaa ggcaaaatcg tggaacaagg
     5641 taagcatgcc gacctgatgg aggagagtgc catctacgca gagatttata actctcagtt
     5701 attacatact tccgaagcgc caacgagtgg cgagacgttg gaagcagaga aggaaggtgc
     5761 actatgatgc gctttggcga aatgctaaat aaagaaacca tcaagccgcg caacctgagc
     5821 gagacgctgg cgcgtcttgg tcaatatttc gtccagtact ggtatatgat cctactggca
     5881 gttctcttcg tggtggtttc gacctggacg caggtgacta ctcctgaatt gaccggccag
     5941 gcgacggatt gtttcctcgt tccggcgggc gcttcggcac ttgaaggatt cggctccttc
     6001 ggaaaccagg ccggttccag cacacaggcc tccacctgtt accttgccac ggatgatcct
     6061 tctaccttat cgtttacgcg acagatcgtt tacaacgcct atactctggg tggcttccaa
     6121 acccctgacc ccttgagcat gaccagtgat caacgcattg aaggtctgac ccgcttgatc
     6181 attgtcatgg tcgccttatt tgttctcagc tctgtactga ccggcgcgac cttcttcact
     6241 atggcatgga cgggacaaca tgtattgcgc gtcttgcgag tgcaggtgtt cgaacaatta
     6301 cacaaactct cgctcagtta ctactcggag catgaagcgg gcgatttgat gagccgcatc
     6361 accaacgata ccaccgccat cgagcaggca ttttcattcg cactggtcaa tgtgttcagt
     6421 ggcattttgt tgatcgtttg ggtatcctat aacatgctta ccacgaacct gcccttcgcc
     6481 ttgctctctt tagctgtagc cccaatcatg ttcctcgcca cctattattt ctctgaacag
     6541 gcgcgcaagg ccttccgtgt ttcgcgcgaa gagatcggga atgtcaatgc ggaattgcag
     6601 gaaagtatcg ctgcggtgcg tgaagtgcag gccttcaatc gcgcagatga aaatatcgaa
     6661 cagttcaagg aagtcaacgc tgcgaaccgc gacgccaatg tgcgcgctgt ggcatacaca
     6721 tctgcccttg ccccgactct ggaagcctta tcttatgtag cgttggcaat cgtcacaggt
     6781 gttggaggat ggtatttact aaagggtcag actcttatgg gcaccactgt gaccctgggt
     6841 attgttgtga ctttcctcgg ttacgtccag cgcttcaacc agccgattca acagatcgcg
     6901 gtgctttgga ccaatattca aaacgccatt gcaggggcag agcgcatctt cacgattctc
     6961 gatgagaagc ccgctgtaaa taacaaaccg gctgcgaaag agatgccaga gatcaaagga
     7021 ttggtcgagt tcgaaaatgc aacgcattct tatgaagatg gcgtacctgt gttgaaagat
     7081 gtttccttta cagctcaacc cggacaaacg attgcgattg tcggtcccac cggcgcgggc
     7141 aagacgacga ttatcaatct gatcccgcgc ttttatgatg tgaccagcgg cgccgttaag
     7201 atcgatggcg tcgacgtacg cgacgtcagc atgaagtcac tgcgcgagca aatcggcatc
     7261 gtgcttcaag acaccttcct cttcagccag agcgtgatgg aaaacgtccg ctttgggcga
     7321 ccggacgcga cagacgatga agtgaaggct gcgatcagac tggcaaatgc agattcgttc
     7381 attgaacgcc tgccagaaaa atatgagact gttttaggcg aacgcggctc cggcttgagc
     7441 caggggcagc gtcagctatt gtctattgcc cgtgctgcgc ttgcagaccc gcgcatcctg
     7501 attttggatg aggccacctc cagcgtggac acgcgaaccg aacgtctcat ccagacggcg
     7561 ttcgacgagc tgctcaaagg acgcacagcg tttgtcatcg cacatcgttt atccactatt
     7621 cgaaatgcgg atgtgatcct ggtcttgaaa gatggtcaga tcatcgaacg cggcaggcat
     7681 gatgaactac tggcaaaaga aggtttctat tacgatctgt acatgagcca gtttaaaaaa
     7741 caagaagaaa tggaagttga gtaattcgca aaaagaaaat cccctttcca tcggaaaggg
     7801 gattttgatt ctataaggtt atacttcact gaagacatta tttcgaggag aaatccatga
     7861 ctctatcccc tgcattgact tatcttgaag aacactatga cagattccag gcagagctgg
     7921 tcgaactcct gcgtatccct tccatttcgc atgacccagc gcacaaagaa gatatggatg
     7981 aagctgcgaa ttggcttgca aacaaacttc gttcgatggg tatcaataac gtagagatac
     8041 ttcccacagc agggcattcg gtggtttatg gagagtcttt gaagggtggc gactcaactc
     8101 ctaccgtatt aatttatgga cactatgatg ttcaatcttc ggagccgttg aaagattgga
     8161 atagtgagcc atttgagccg accattcgag acggaaacct gtatgcgcgc ggcgcgacgg
     8221 atatgaaagg acagactctg gcggcagtaa acgctgtcga agccatcctg aagtccgaga
     8281 acctgccgat caatatcaag ttcttgattg aaggcgaaga ggaaattggc tcaaggcact
     8341 tgaatgagtt tttggtagcg catcgcgaaa aacttgcttg tgatttttcc ctgaacaccg
     8401 atgcaggtgg tatgcccgat gcaaatactc cgtcaatttg ttattcgctg cgaggtggcg
     8461 ttgcctttgt tttacagatt ttcggaccca gccaggatct ccattcaggc gaatatggcg
     8521 gagtggttca aaacccgatc catgtcttga gcaaattgat tgcgggtttg catgacgaaa
     8581 atggacacgt cacattgcct ggtttttacg acaaggtccg aaacttagac gatgaagaac
     8641 gcgccgaaat ggcacggctt cccttcgatg aagagtttct tctcaaacat tcaggcgcac
     8701 ctgcattatg gggcgagccg gaatatatcc ccgccgaacg gattggagcg cgtcctacag
     8761 tagaagttgt gcaatttgaa gccgggcagc cgaaatccgc catccccgcc aaggccactg
     8821 cccgtttttc atttcggctt gtcccggatc aggatccaaa cgaaatccat caacagttca
     8881 aacactattt agaaactcat gctcctgcta ctgtgaactg ggatcttgaa atgatcattg
     8941 gtaaccctgc tgtcattacc gatcgcaaat ctcgcgaagt gctcgcgatg aaggatgctc
     9001 ttcaaactgc ttttggaacg ccaccaattt tccaaagatc aggtggaggg atcggtgcgg
     9061 tattgatgtt taaacaaacg ctgggtgttg actcggtttt gaccggcttt tcgctatttg
     9121 atgacaacct tcatggtccg aatgagaaat tacatctgcc cacctggaag aaaggcatgg
     9181 cagcactggt ccatttcttc cataatttgg tgaatagata actgggtatg ttcaatatat
     9241 aaaagtctct cacgattata gtgagagact tttgattttc ttcaggtttc cttctttaat
     9301 tcttcaagat gcggatcagg ctgggtcgga attgaagaca gtacgttcaa gcctcttaat
     9361 aacggagtca ttgtcatgcc ttgaatcatg agttctacaa ggcaagtatt catttatatc
     9421 cttaccaacg acagcttgcg atttcattct ttcatccttc ttctttcatc ttctatggta
     9481 tccttaactt aaccaacctt actcctggag cctgtgcatg tacgttgcca ttgaaggggt
     9541 tatcggagtt ggaaagacca ctctggcccg ccttctacaa tctacgttca acgccgaaat
     9601 cctactcgag gtctttgaag agaatccatt cctttctgat ttttacgccg atagaaatcg
     9661 ttacgccttc cagacccaaa tcttcttttt gttgagtcgc tatcatcagc aacgccgaac
     9721 tgtacttgat atgctctcta ctggaacaaa tcttttcgcc gattacacgt tcgccaagga
     9781 cgcgctcttc gcgcgcatca atctgaaagg cgacgaactc gacatgtact ataaagtcca
     9841 tgaggccctg gcagagaaga ttcaaaaacc ggacctgctc gtctatttgc aagcgaccac
     9901 cgatacgctc atgcagcgga ttgcccaacg cgatcgttcc tatgaacgtc agatggaacg
     9961 ctcctacatc aatgaactca atttggctta tgatgaattc ttttcaaagc ctttcgacca
    10021 tacgccggtg ttaaccatcg actccaacaa cttgaacgtt gttcaaaacc cggaagattt
    10081 gaaactgata gagaaccgta tcagacaaag cctcagcctg cctccgtttc aacagacatt
    10141 gcctttgcct cattaagggt atagaaggaa ttatccatgc gagtcacgcg tgaatcattg
    10201 atccgaatcg ccaaagagac tgcgcagcaa cgcgccttca atgaccgcga tatcattgct
    10261 gcttatttat ctggctcgct tgcgtctgac gaaaaggatc cgttattggg gggcaccgcg
    10321 gatattgata tcatctttgt ccatgtggat gatccgctta tcagacgtga attcgtcaag
    10381 ctcactcctg attttcatgt agatatcagt catcgcgcca aagctgaatt taagaaacct
    10441 cgtgaactgc gtgtagaccc atggttgggt tgggaaatgt acgatcccat gctgcttttc
    10501 gagcgtgaaa agttctttga gtttattcag gccggcctca gggctggctt tgaattcaat
    10561 gctcccgccc ctgcccttca tcgctccagg ttattgcttg cgaaggcgcg acagctctgg
    10621 agggaattgc tggcaattga ggatgtcgtc acaccaggcg acgttctgca gtatatggac
    10681 tccctctacc atgctgtgaa tgcagtcgcc gagttgtcgg gtccgcccct tcaggaaaga
    10741 cgcttgttat tggattttgc ccaaagagcc gagacagcgc aacgccccga catggatgcg
    10801 gccctcatcg gcttattagg cggatcggca ctggattcgg cctccatcag tgaatggctc
    10861 cctgaatgga aactggcttt tgaatcggca atgcaggata gtgacgtaga cccgcgcatc
    10921 cattcttcac gcatcaatta ttacgagaaa gcgcttcgag agattcttgc aaatgacaat
    10981 cctcaggctg ccctttggcc cttgtggcaa acctggactt tggctgccaa ggtactgccc
    11041 aattttgcgc aggatccctg gcggggcgtc tgtggtcagc tcggtttcac aagtcaaggc
    11101 atggaggaac atgtaaccgg gctcgatcac ttcctcgatg aagtggaagt attgctcgat
    11161 gagctgactg cgcagtatgg attggaaact tctacgactt tataaattcg tagttgcgac
    11221 ttaagtcgct ctatttgcta aattattgga cgactgcacg cagtgcaggt gagcaatctc
    11281 aactaacttt taatctctta tttgaatgaa attatgagat tgccacgtcg ccgctacgcg
    11341 gctcctcgca atgacatatg aaaaggtgaa tatgacccgt gatgaagcgt tagcactcgt
    11401 tcgtgaatat gtaaaaaatg aaggcctggt aagacacatg ctttccgttg aagctgccat
    11461 gcgtttttac gccgaaaaat ttggtgaaga ccttgaaaca tggggattga tcggtttact
    11521 gcacgatttt gactgggaaa tccacccgac ccttgaacaa catccacagg acggcgcgcc
    11581 aattttacgc gagcgcggcg tccctgaaga gatcattcag gacattttga gccatgcaga
    11641 tcacctgaac ttgcctcgcg ataccatacg ccgcaaagcc atctgtgcct gtgacgagat
    11701 caccggactc attaccgcgg ttgcgctggt ccgcccctct cgttcgttat atgatctgga
    11761 agcatcctcc gttaaaaaga aatggaaaga taaagctttt gcagccggaa cgtcccgcac
    11821 cgaaatgggg cacgcggcgc aggagttcgg tatcgagcta tgggaacata ctgggaatgt
    11881 gattcaagcc atgcgcagga tcgctccaga gttgggattg gtggggaata ttcagcagcc
    11941 atcctcctga tggcttcatc aaccctggct ttctaacgtt atcatagaaa aatccacttt
    12001 tgttcgttta attaatagtt tcgagagtac ccctttcgat gatttgccat tccatacaac
    12061 caagcctata atcaacctat catgtcggta actgacgaaa tcaaagcccg cataaatatc
    12121 gtagaccttg tctctgaagc tggcgtcaaa ctgcgccatg cgggcaagaa ctatacaggc
    12181 ttttgtccct tccatgaaaa caagaagacg cctgcgtttg tcgtatggcc cgagtctggc
    12241 acatggcgct gcttcggtca gtgtaatgaa ggtggcgatg tcttcaagtt tgtaatgaag
    12301 cgtgaaaatc ttgatttcaa ggatgcattg aacacgct
//
LOCUS       DASTJP010000807         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1132777, whole genome shotgun sequence.
ACCESSION   DASTJP010000807 DASTJP010000000
VERSION     DASTJP010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132777"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..2009
                     /locus_tag="VFQ23_23645"
     CDS             78..2009
                     /locus_tag="VFQ23_23645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HET9909661.1"
                     /translation="MAIQTAELKPAVASSPLFGFWKSFSKNRIAVVGLFMLIGIMILA
                     IFAPILAPYDPRSSAGIQTSDIYNPPSAQHWFGTDDAGKDVLSSFIYGSRVSLIVGFF
                     AAFISIFIGGVIGIVAGFYGGRVENLLMRFTDTMLVIPDLPLIVVIVALTRPSLLNII
                     FVIGLLGWTTTARIVRSQTLAVKSRKFVLRARAIGAGNWHIILNHILPLVMPLLVVNA
                     ILVISLAILNESTLSFLGLGDPTATSWGQMLNFAFGRGAMSAGAWWALFAPGFGIVWV
                     VLALTLLGHGLEQVLNPRLESHHLMPGRPTIQTDRDAKLQTRKRRMDVPVLLDVQNLS
                     VNYVNDDRIANAVHNVSFQLHEGELLGLVGESGCGKTTLMMALMRLLPASGEISNGHV
                     YFRGQDLATMGEDEIADLRWSGISIIFQGAMNALNPVRTVGDQIAEAILRHTPSFPPG
                     ALDTRVVELLDLVGISAEHKNHFPHQYSGGMRQRAMIAMALSCSPQVVIADEPTTALD
                     VMIQAQILELLDDLRKKLGLAIIFVTHDLGVVAEICDSVLVMYGGVTAEYASVDAIYN
                     DSRHPYTQELLKAFPDLSRPEKRLISIPGYPPKLDALPAGCRFAPRCPLAFERCFSEM
                     PALHSLHDGDHLASCHLVE"
     gene            1969..3018
                     /locus_tag="VFQ23_23650"
     CDS             1969..3018
                     /locus_tag="VFQ23_23650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909662.1"
                     /translation="MTAIILRVATWLNKALSMSNDYILEVKNLKKYFPAGRPGWFSRN
                     DKFVHAVDDVDLQLLRGEVIALVGESGCGKSTLSLTLMGLEEATEGTILFEGKDITHI
                     SEEERKKIRQRIQMVFQDPYESLNPTQTIEEIVTEPLLVHALVPDHNERRERVRRALE
                     DSGLKPAESYLHRFPHQLSGGQRQRVVIAGALVLEPHLLLADEPVSMLDVSIRAEIIN
                     LLADLRKSRGISVIFITHDLGTVGYFADKIAVMYLGRIVEIGKMTDVLKTPQHPYTQA
                     LLSVIPVPNPRLRKQRVILQGETPNPIDIPSGCRFHPRCPVAIEICRHQDPSLIQVSE
                     THAAACLLLNKVSVT"
     gene            3019..>3700
                     /locus_tag="VFQ23_23655"
     CDS             3019..>3700
                     /locus_tag="VFQ23_23655"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9909663.1"
                     /translation="MDITSLQNPRIKYIVRLRDDKRQRQSDGLMLVEGFDEIQLALSA
                     GYRPQTLLSAPELTSRELEVVNAEAITVTQAVFEKISYRQNPDGWLAIFPLPHISLDD
                     LKLSETPLLIVAESIEKPGNLGAILRTADAAGVDALFVCDPRVDAYSPNVVRASRGTL
                     FTVPVIEINSTQAVIYLQRNGIRVLAATPAAEEEYTRQDLRGPLAVVVGTEAEGLSDF
                     WLSQADMRV"
BASE COUNT          849 a          923 c          981 g          947 t
ORIGIN      
        1 tcagcgtggt ggcagcaaat ctgatagcag accttaccta ctcttttctt gacccgcgcg
       61 tgcaggtgga ataaacgatg gctattcaaa ccgcggaact caaaccagcc gtcgcttcct
      121 cgccattatt tggtttctgg aaaagtttca gtaaaaaccg gattgcagtc gtgggattgt
      181 tcatgctgat cgggatcatg attcttgcaa tctttgcacc catcctggcg ccctacgacc
      241 ccaggtcatc ggcaggaatt cagaccagtg acatttataa cccgcccagc gcgcagcatt
      301 ggttcggaac ggatgacgcc ggaaaagatg tcctttccag tttcatctat ggttcacgcg
      361 tttcattgat cgtgggtttc tttgctgcat tcatttcaat tttcattggc ggggtaatcg
      421 ggattgtggc agggttttat ggtggacgag tcgaaaacct tctgatgcgg tttacagata
      481 ccatgctggt cataccggat ttaccgttga tagtggtaat cgtggcgctg acccgcccca
      541 gcctgttaaa cattattttt gtgattggct tattgggctg gaccacgacc gcacgcattg
      601 tgcgttcgca aaccctggcc gtcaagtcgc gaaaatttgt attgcgcgct cgtgccatcg
      661 gggcgggtaa ttggcatatt atcctcaatc acattctgcc actcgtgatg ccattgctcg
      721 ttgtcaacgc aattctcgtg atttcgctgg cgattctgaa tgaatccact ttatcctttc
      781 tgggtcttgg cgacccgacc gcaacgtcct ggggacagat gctcaacttt gcttttgggc
      841 gtggtgccat gagcgcgggt gcctggtggg ccttgtttgc acctggcttt ggaattgtat
      901 gggtggtcct cgccttgacc cttctggggc atgggctgga acaggtatta aacccaaggc
      961 tggaatcaca tcacttgatg ccgggcaggc caacgatcca gaccgataga gatgcaaaac
     1021 tccagacgag aaaaaggcga atggatgtgc cagtcctgct ggatgtgcaa aatctgtcgg
     1081 tcaattacgt caacgatgac aggattgcaa acgccgtgca caatgtcagt tttcagttgc
     1141 atgaaggtga attgctgggg ctggttggag aaagcggctg tggcaagacc acattgatga
     1201 tggcgcttat gcggttgcta ccagcctcag gcgagatcag caatggacat gtctatttcc
     1261 gcggccagga cctggcaacc atgggcgaag acgaaattgc agatttacgt tggagtggga
     1321 tatccattat ttttcagggc gctatgaacg cgctcaaccc tgtgcgcacc gtgggtgatc
     1381 agattgctga ggcaatccta agacacactc catctttccc gccaggagca cttgatacgc
     1441 gggtcgttga attgctggac ctggttggca tttctgccga acataagaat catttccctc
     1501 atcaatattc gggaggcatg cgacagcgtg ccatgatcgc catggcgctc tcgtgcagtc
     1561 ctcaggtagt cattgcagat gagccaacca cagctctgga tgtgatgatc caggcgcaga
     1621 ttctggaact gctggatgat ttgcggaaaa agctgggtct tgccatcatt ttcgtcacgc
     1681 atgatcttgg ggttgtagct gagatctgcg actcggtgct ggtgatgtat ggcggcgtga
     1741 cagccgaata cgcaagcgtc gacgcaattt acaatgattc gcggcatccg tatacccagg
     1801 aattactcaa ggctttccct gacctttcca gacctgaaaa aagactgatt tccattccag
     1861 gatatccgcc caagctggat gcgctacctg ccgggtgccg ctttgcgccg cgctgcccgc
     1921 tggcgtttga acgatgcttt agcgaaatgc cggcattgca ctcactccat gacggcgatc
     1981 atcttgcgag ttgccacctg gttgaataaa gccctatcta tgtccaacga ttacattctt
     2041 gaagtcaaaa acctgaaaaa atattttcca gcgggaaggc cgggctggtt ttcgcgaaat
     2101 gacaaattcg ttcatgccgt ggatgatgtg gatttgcagt tgcttcgtgg ggaagtgatc
     2161 gctctggttg gcgaaagcgg ctgcggtaaa tccacacttt cgcttactct gatgggactg
     2221 gaggaagcca ccgagggaac gattctattt gaaggcaagg atattacgca tatcagcgaa
     2281 gaagaacgca agaaaattcg tcaacgaatt cagatggttt ttcaggatcc gtatgaatcc
     2341 ctgaacccaa cacagaccat tgaagaaatt gtcactgaac cattgctggt tcatgcactt
     2401 gttcccgacc acaatgaacg tcgtgagcgc gtccggcgcg cgctggaaga ttcgggactg
     2461 aaacccgcag aaagctatct tcatcgtttt ccgcatcagt tatccggtgg gcagcgacag
     2521 cgcgtggtga ttgctggagc gctcgttctg gagccacacc ttttgctggc agacgagcca
     2581 gtctccatgt tggatgtctc catccgcgca gaaattatca acctgctggc cgatttacga
     2641 aaatcgcgcg ggatttcagt catctttatc acacatgatc ttgggacggt tggttatttt
     2701 gcggataaga ttgcagtcat gtatctgggc cgtatcgttg agatcggaaa aatgaccgac
     2761 gtgctgaaga ctccccagca tccgtacaca caggccttgc tttcggttat acctgttccc
     2821 aatccgcgcc tgcgcaaaca gcgggtgatc ttacaagggg aaacacccaa tccaattgat
     2881 attccatcgg ggtgccgttt tcatccacgg tgccccgttg cgatcgaaat ttgcaggcac
     2941 caggatccta gcttgataca ggtcagcgaa acgcacgcgg cggcatgcct gttattaaac
     3001 aaggtgtctg tcacatagat ggatattacc agcctgcaga acccgcgtat caaatatatc
     3061 gtccggctgc gcgacgataa acgacaacgc cagagtgatg gtttgatgtt agtcgaagga
     3121 tttgatgaaa tacaactggc gctttctgcc ggataccggc ctcagacact cctctccgca
     3181 cctgaactca cttcccgtga acttgaagtg gtcaatgctg aagccattac tgttacgcag
     3241 gcagtctttg agaaaatatc ctatcgacag aatccggatg gctggctggc gatcttccct
     3301 ctcccacata tttctctgga cgacttgaag ctcagtgaaa caccattact tatcgtggcg
     3361 gaatcgatcg agaagccggg taatcttggg gcgattcttc gcactgcaga tgctgctggc
     3421 gtcgatgcac tctttgtctg tgacccccgc gtcgatgctt acagccccaa tgttgttcgc
     3481 gcgtcgcgcg gcacactgtt tacagtcccg gttattgaga taaacagtac tcaagcagtt
     3541 atttatttgc aacgcaatgg aattcgtgtt cttgcggcta ctcccgctgc ggaagaagaa
     3601 tatacacggc aggatttgcg cggtccgctg gccgttgtgg tgggcacgga agccgaggga
     3661 ttaagcgact tctggttaag tcaggcagat atgagagtaa
//
LOCUS       DASTJP010000808         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_498183, whole genome shotgun sequence.
ACCESSION   DASTJP010000808 DASTJP010000000
VERSION     DASTJP010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498183"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..937
                     /locus_tag="VFQ23_23660"
     CDS             176..937
                     /locus_tag="VFQ23_23660"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9909664.1"
                     /translation="MSTHPVLNPEAEFIQKRPLKEDIFDVLHEKIISGTYKPGDWLRQ
                     DDIATQLGVSMTPVREALDLLVSAGLAERVPYRGVRVREASTKDVVEAYGLRLVLEVM
                     IAQEAARNITPEQISDLERMIDEMKKHNSLKDMAYARQLSREFHSAVAETTRNDLLIK
                     VYSVVANAFPDWLLYEALFRIPEILAESMSDTHSEHIAMVDALKEHNGDLAAEKFIEH
                     VLESGRWLKEYLDIPAELLLEKEEQALYLLKKTTK"
     gene            950..1600
                     /locus_tag="VFQ23_23665"
     CDS             950..1600
                     /locus_tag="VFQ23_23665"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HET9909665.1"
                     /translation="MSEELYKQMAQCIIEGEKEIAIELAEKSIELQMHPLDTITKGFV
                     VGVNYIGDQFGKGEAFLPELVMAGEAMKAAVAVLEPELLKLGEAREMMGRVVLATVEG
                     DIHEIGKTLVGTMLSASGFEVTDLGVDQPADKIIGKALEIDAQIIGMSALLTTTMIRQ
                     REVIEELDKEGLRPRIKVIVGGAPITKDWAEKIKADGTAEDAVGAVQLAKKLLGKE"
     gene            1661..3142
                     /locus_tag="VFQ23_23670"
     CDS             1661..3142
                     /locus_tag="VFQ23_23670"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HET9909666.1"
                     /translation="MQPKLTLLTDDIIERILDEAYQLLLKPGVKVNNEEARELLASAG
                     AQVDAETNVVHIPQQIVRKALESAPREFYLYDYDGNPTVKYGGDAVHFDPGSSGIAML
                     NPETLEHDTTETPHLLKLIKVAEQLSQYDAQSTAVVCHDVPQQVQDSYRLYLALLYSK
                     KPIVTGAFTNKTVTEMIDMLAILAGGQEAAREKPRAIFDVCPAPPLIWSNFASGNLIA
                     LARAGVPAEIVSVPLSGAAAPVTLLGTVTQHTAECLAGITIHQLANAGSPIVWGGAAC
                     IFDMKKGATPMGAVETAMLDCSYAQVAKSLNMPTHTYLGATDSKLVDAQAGLESGITA
                     MIGALSGINMISGSGMLDFLSCHSAEKLVIDAEGIAMAKRMIEGVKLHTETLATGFYD
                     DNINFKGGDFLKQRITMQLFRKEQHLPSNVIDRDSMRNWKEAGSLDTYGRAKVMVTQL
                     LASYKKPELDPVKERELRDFMLDLAKKAGMDTLPVIEEITAPA"
BASE COUNT          928 a          817 c          863 g          778 t
ORIGIN      
        1 cttgcctgga tgttgaaaga agctccagtt gttcaggccc ctatcctcaa tgtagaagag
       61 tcaataaaaa ccgtagcata gcagtctcag tttttcaggg tgtaacttat tcggttcaaa
      121 cttcctgtac taaaaattat atataattta taatcacgga cacttctaag gaaatatgtc
      181 tacgcatccc gttttaaacc cggaagctga attcattcag aaacggcctc tcaaagagga
      241 catttttgat gtactgcacg agaaaatcat ctcaggtacg tataaacctg gcgactggct
      301 tcggcaggac gatatcgcca cccagcttgg agtcagtatg actccggtcc gggaggcgct
      361 ggatttactc gtttccgccg ggttggcaga gcgtgtcccc tatcgggggg tacgcgtccg
      421 agaagcgtcg acgaaagacg tagtcgaagc ctatggatta aggcttgtcc ttgaagtaat
      481 gatcgcccag gaagcagcca ggaacatcac cccggagcag atttcggatc tggaacgaat
      541 gattgacgaa atgaaaaagc ataattcgct gaaagacatg gcgtatgccc ggcagttgag
      601 tcgtgaattc cattccgctg ttgcagaaac aacgagaaat gatctactta tcaaggtcta
      661 tagcgtggtt gccaatgcct tccccgattg gttactttat gaggcattgt ttcgtatccc
      721 tgaaatcctg gcagagagca tgtctgatac tcacagtgaa catattgcca tggtggatgc
      781 actcaaggaa cacaacggag atttggcagc tgaaaaattt attgagcatg tgctggaatc
      841 tggcaggtgg ttgaaggaat acctcgatat tcctgcggaa ttattgcttg aaaaagaaga
      901 acaggcgctg tacctattga agaaaacaac gaaataagga gtaatttgca tgtcggaaga
      961 actatacaaa caaatggcgc agtgcatcat cgaaggcgaa aaagagattg cgatcgagct
     1021 tgcggaaaaa tcgatcgaac ttcaaatgca ccccctggat acaatcacga aaggatttgt
     1081 ggtcggcgtt aattacatag gcgaccagtt cggcaaaggc gaagcctttt tgcctgaact
     1141 cgtgatggcg ggcgaagcga tgaaagctgc ggtagcggtg ctcgaaccgg aattattgaa
     1201 acttggcgag gcacgcgaaa tgatgggacg tgtggtactt gcaaccgtcg aaggtgacat
     1261 tcatgagatt ggcaaaacat tagtcggcac catgctgagc gcttcgggct ttgaagtgac
     1321 cgatctcggc gtggaccaac ccgctgacaa gattatcggc aaagcactag aaatcgacgc
     1381 gcagatcatt ggcatgagtg cgttgttgac aacgaccatg attcgccagc gtgaagttat
     1441 tgaggaattg gataaagaag gtctgcgacc tcgcatcaag gtgatcgtgg gcggcgcgcc
     1501 gatcacaaag gattgggcag agaagatcaa ggcggacggc accgcggaag acgcagtcgg
     1561 cgcggtgcaa ttagcaaaga agttacttgg taaggaataa tgaatttgtc cgcagctgat
     1621 gcagtctgcg gatgtttttt tctttaggaa agaggtagtt atgcagccaa aactcacgct
     1681 cctcacggat gacattattg agcgcatcct ggatgaagcc tatcaacttt tgttaaagcc
     1741 tggggtcaaa gttaataacg aagaagcacg tgagctactt gcttcagccg gagcgcaggt
     1801 tgatgcggaa acaaacgtag ttcacatccc tcaacaaatc gtccgcaaag cgttggaatc
     1861 tgccccgcgc gaattttatc tttatgacta tgatggcaat cccacagtca aatacggcgg
     1921 tgatgcggtt catttcgacc ccggctcttc cggcattgcc atgctcaacc ctgaaacact
     1981 tgagcacgac actacagaaa ccccacatct cctcaaactt attaaagtag cagagcaatt
     2041 atcgcaatat gatgcgcaat ccaccgccgt agtttgccac gatgtgccac aacaagttca
     2101 ggattcgtat cggctgtatc tggccttgct ctattcaaaa aaaccaattg tcacaggcgc
     2161 gttcacaaac aaaaccgtga cggagatgat cgatatgctt gccattctgg caggcgggca
     2221 ggaggctgca cgcgaaaagc cgcgcgccat ctttgatgtt tgccccgctc ccccactgat
     2281 ctggagcaac ttcgcttcgg gcaacttgat tgcattggcg cgcgcaggtg tgccggctga
     2341 aatcgtctct gttccgcttt cgggggcggc tgctcccgtt acgctgctgg gaacggtaac
     2401 acaacacacc gcggagtgtc tggcgggtat cacgattcac cagctggcaa acgcaggttc
     2461 acccattgtt tggggcggcg cggcctgtat cttcgacatg aaaaaaggcg caacgcccat
     2521 gggcgcggtt gagactgcca tgctcgattg ctcatatgcc caggttgcaa aatcactaaa
     2581 catgccaacg catacttacc ttggcgccac tgactcaaaa ctggtagacg cgcaggccgg
     2641 tctcgaaagt ggcatcacgg ctatgatcgg cgcgttgagc ggcatcaata tgatctccgg
     2701 ctccggcatg ctagatttcc tctcctgcca cagcgcggaa aaactcgtca tcgacgcgga
     2761 aggcattgcc atggcgaagc gcatgatcga aggtgtaaaa ctccataccg aaacactcgc
     2821 taccggcttc tacgatgaca acatcaactt caagggtggc gattttctta agcaaagaat
     2881 tacaatgcaa ctgttccgca aggaacagca cctgcccagc aatgtgattg accgcgactc
     2941 catgcgaaac tggaaggaag ctggcagttt ggatacgtat gggcgcgcca aagtgatggt
     3001 gactcaactg ctcgcctctt ataaaaagcc tgaacttgat cccgtcaaag aaagagaact
     3061 ccgcgacttc atgcttgatc ttgctaaaaa agccggcatg gacacgttgc ctgttatcga
     3121 agaaatcacc gctcccgcct aaagtgtgat gtcttcattc gatgcactcc tcaaggaaac
     3181 aggacggccc gcaaagcaaa cgttcgccgc ttagtcgttc cttaggattt tgtctgagca
     3241 ttttgagatc agcaattgaa caagctgtaa aatagaagct ctggtacctt cggtactgtc
     3301 acccgtaata ccaatcacca tcttaaagga gaacgctatg cttaaacaag cacatgcaat
     3361 ttcagaagaa ctcattgaat ggcggc
//
LOCUS       DASTJP010000809         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_498261, whole genome shotgun sequence.
ACCESSION   DASTJP010000809 DASTJP010000000
VERSION     DASTJP010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498261"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFQ23_23675"
     CDS             complement(<1..213)
                     /locus_tag="VFQ23_23675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909667.1"
                     /translation="MADTKTCSKCNGTMTQGRILKYNEYTPGGKYMYVFAADDESGPD
                     LSKMFSGKPLSKSRKPLVAFCCEQCGF"
     gene            complement(275..2326)
                     /locus_tag="VFQ23_23680"
     CDS             complement(275..2326)
                     /locus_tag="VFQ23_23680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9909668.1"
                     /translation="MSLINVSSLSKSFGAEDLFAGVTFSVAKGARLALVGPNGIGKTT
                     LLRILIGQEEPSSGTVTRAKNLRVGYLSQEADFELQGVLWDVCLEPFADLIRMQKEVE
                     RLEDEMSDPAKREEALVRYGSVQHEFERRGGYVYQVRIKQVLTGLGFDEPDFHMSLDH
                     LSGGQRTRAHLARLLLSNPDLLLLDEPTNHLDIKAVEWLEGYLTQWEGAAIVVSHDRY
                     FLDHACNALLEMAISGSEYYRGNYTTYLNEREIRWNHRFEVFESEKEKLLKELDYIKK
                     NISGQNTLQAKGRLKRLTRAVQAVEQVGISAVVNSNWSQLDVETTTSSFSVDEAERRV
                     RALRPPRRTMPDLHLHLRSTQRSGDLVIRTKDLQVGYPAENGLAEKLLFRSPNIELRR
                     TDCAALIGPNGAGKSTFLKTILGSLAPLAGEVILGASLHVGYFAQAHEGLDAEKTVLD
                     EILEASPGMLPFQGREYLGKYLFSGDDAFKQVSMLSGGERGRLALAKLALEDTNLLLL
                     DEPTNHLDIPSQEVLQSVLDSYNGTILLVTHDRYLVDALATQIWEIDPDESHLSVFNG
                     TYSQMKEESAALSLSKGEKEAYRLAAQESTVVSDTRTSTNGARRSRNKENKEERRKIA
                     QLQELENTIAELEAQLANLASQLESPFVKPNEAAKLGTEYERVQKEMDQKLDEWEKMQ
                     T"
BASE COUNT          637 a          724 c          657 g          702 t
ORIGIN      
        1 aaacccacat tgctcacaac aaaatgcgac cagaggttta cgcgattttg acagtggctt
       61 gcccgaaaac attttggaga gatcaggccc ggattcatca tctgcagcga agacgtacat
      121 atatttcccg cctggagtat attcgttgta cttcaaaatc cgcccctgag tcattgtacc
      181 gttgcatttc gaacaagttt ttgtgtctgc catgttcgtc tccaatggta gagaggataa
      241 acgattggcg tagtttatcg tccatcctct actatcaggt ttgcatcttc tcccattcat
      301 ccagcttctg atccatctct ttttggacgc gttcatattc tgtgcctagt ttcgcggctt
      361 cattcggctt cacaaacgga ctctctaact gggaagcgag atttgccagt tgcgcttcga
      421 gttcggcaat ggtattttcc aattcctgca gctgagcaat ctttcttcgc tcctctttgt
      481 tttccttgtt ccttgatctt cgagctccgt tagtagaggt tcgtgtatca gaaactaccg
      541 tggattcttg cgcggcaagt cgataggctt ctttttctcc cttcgataaa ctcagggcgg
      601 cgctttcctc tttcatttga gaatatgttc cattgaagac agatagatgc gattcatccg
      661 ggtcaatttc ccagatttgg gtcgccagcg catcgaccag ataacggtca tgtgtgacaa
      721 gcaggatcgt cccgttgtat gaatccaaca cggattggag aacttcctgt gaaggaatat
      781 ccaaatgatt agtcggctca tcgagtaaca acaggtttgt atcttccagg gcaagcttgg
      841 ctaaagctaa tcgtccgcgt tcaccgccag agagcatgga aacttgcttg aacgcgtcat
      901 cacccgagaa gaggtatttg ccgagatatt cacgtccctg gaaaggcagc atgccgggcg
      961 aagcttccag gatttcatca aggaccgttt tctcggcgtc gagtccctca tgcgcctgcg
     1021 cgaaatagcc gacgtgcaga ctcgccccaa ggatcacctc gccagccaat ggcgcaagcg
     1081 agcccaggat cgtcttgaga aatgtcgact tccccgcgcc attaggtccg atcaacgccg
     1141 cgcagtctgt tcgtcgcagt tcaatattcg gcgaacggaa aagtaatttc tctgcgagtc
     1201 cattttcggc cgggtaacct acttgtaagt ctttggtacg aatcaccaaa tcgccagaac
     1261 gctgtgtaga acgcagatgc aggtgcagat caggcatggt gcgccgcggt ggccgcaacg
     1321 cacggacgcg tcgttcggct tcgtccacgc tgaaggaact ggtggtcgtt tccacatcca
     1381 attgagacca attcgaattg acgaccgcac ttatgccaac ctgttcaaca gcttgcaccg
     1441 cgcgtgtaag ccgcttgagc ctgcccttcg cttgcagcgt gttctgcccc gaaatatttt
     1501 tcttgatgta gtcaagctct ttgagaagtt tttccttctc gctttcaaaa acttcaaacc
     1561 gatggttcca acggatctca cgctcgttca gataggtggt gtaattcccg cgataatatt
     1621 ctgaaccgct gatcgccatc tcaagcaacg cattacaggc atggtccaga aaataacgat
     1681 catgtgaaac gacgatcgcc gcgccttccc attgcgttag atagccttcc agccattcaa
     1741 ccgctttgat atcgagatga ttggtcggct cgtccagcag caacaaatcg ggattggata
     1801 ggagaagcct cgctaaatgg gcacgcgtgc gttgcccgcc ggagagatgg tccaacgaca
     1861 tatgaaagtc gggttcatca aagcccagac cggtgaggac ttgctttatg cggacttgat
     1921 agacgtatcc gccgcgccgt tcgaactcat gctgtacact tccataccgg accagggcct
     1981 cttcccgttt cgctgggtca gacatttcat cttcgagtcg ttccacttcc ttctgcatgc
     2041 ggatgaggtc cgcgaacggc tcaaggcaca catcccataa cacgccctgc agttcaaaat
     2101 cagcttcctg tgaaaggtac cccacacgaa gattcttcgc gcgcgtaacc gttccggaag
     2161 atggttcttc ctgcccgatc aggattctaa gaagagtcgt tttgccaata ccgttcggac
     2221 caacgagcgc taaccgcgca cctttggcaa ccgaaaacgt gacaccggcg aagaggtctt
     2281 ctgcgccgaa ggatttggag agagaactta cattaatcag tgacataatt gtttgcgtag
     2341 gggtacagcg gagaattgca tgtcctcttg acgaacaccc ggctcgctgt gcccttacgg
     2401 tgcaaatgcc cccgaattat aaaacaaccc ccacccatcc tccacctgca aagcatcctg
     2461 taagggcaaa tctgacattg agtttttcac atctgccagg cttaggattg tcggatttgg
     2521 gggaggtgcc cgcaaagcgg gcggaggggg ctgattcctt cacttacata cgaaatttac
     2581 ctgttgctat ttagaatcat gagcgcgata atgtttcagc cgagtcgcct gttgaatgta
     2641 ggaggatggt atggaaactc gctcgaccca aagtgcggat cgtcttgcga aaatcagtcg
     2701 aatccttgga ctattatcaa
//
LOCUS       DASTJP010000810         5975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_373848, whole genome shotgun sequence.
ACCESSION   DASTJP010000810 DASTJP010000000
VERSION     DASTJP010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373848"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..473)
                     /locus_tag="VFQ23_23685"
     CDS             complement(204..473)
                     /locus_tag="VFQ23_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909669.1"
                     /translation="MAKQPAFTKYLPLENWFRKQPASKMQIKLTFDKVEEILGNPLPK
                     SATKLATWWTNVQPKIQSHRTAWLNNGWKVKEFDLQARWAKFVRD"
     gene            complement(588..1448)
                     /locus_tag="VFQ23_23690"
     CDS             complement(588..1448)
                     /locus_tag="VFQ23_23690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9909670.1"
                     /translation="MTQVIKYITANGLAFAYYEEGSGPLILLLHGFPDTPHTWFQVQP
                     RLVEAGYRVVAPFMRGYSPSAAPTNNDYSALSLGQDVLALIEALGEKQAIVIGHDWGA
                     FAAYAAANIDSQRISKLITIAIPHPGTLRANLRTLLKAHHFITFQFRKWIVARLKRNN
                     FAEVEAIYRRWSPAWAVMPAEIEPVKQALAAPHGVEHALGYYWSVAGDAMGPRGVRVR
                     RVLQARTAVPTLSIFGDLDGALDNKNIEQTRNYFTAVYELVRIPEAGHFVHREAPEVV
                     LDRILKFIQQ"
     gene            complement(1569..1985)
                     /locus_tag="VFQ23_23695"
     CDS             complement(1569..1985)
                     /locus_tag="VFQ23_23695"
                     /inference="COORDINATES: protein motif:HMM:NF039682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HET9909671.1"
                     /translation="MIDESSISSYITSTFESVENSTNLGYVFFFYRDDHMVPFATIAS
                     SGNEYEKVSRLDRPGVYRLNIGVSRETFQSLFGTDKINVSDYDFTALDLIMPHPDYSA
                     QLFICVLSLSEATFETIKPLLAEAYDIAVKKYNRRH"
     gene            complement(2071..5958)
                     /locus_tag="VFQ23_23700"
     CDS             complement(2071..5958)
                     /locus_tag="VFQ23_23700"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9909672.1"
                     /translation="MATSIPASTLDKFITFGDLLRFLRRRMGITQMELSIAVGYSNTQ
                     ISRLEQNERLPDIPTIEARFVSALGLEEEPKAVARLLDLAANVRREDAPATGLSPYKG
                     LNYFDETDADFFVGRETLTAKLIERVLALTSTRSSHKTRFLAVVGASGSGKSSLARAG
                     LVSALRWNKASTDWRIHILTPTAHPLESLAGTLTQESNSVIATATLMDDLARDERSLQ
                     IFTKRILRSMNGSRLMLVVDQFEELFALCRSEEERAAFIGNLLTAASEVDGPNLVVIT
                     LRADFYAPCANYPRLREALAAHQEYIGAMNEEELRRAIEEPAQRGRWEFEHGLVDLLL
                     HDVGREPGALPLLSHALFETWQRRRGRTMTLSGYTSSGGVRGAIAETAEAVFVDQFTH
                     EQQAIARRIFLRLTELGDEAGAGDTRRRATFDELILKPEEAATTHVVLKALADARLIT
                     TTEGAAEVAHEALIREWPTLLGWLEENRDGLRLHRQLTEAAQDWVAMNHDPDLLYRGA
                     RLVQVSEWSSTHEEEMNALEVEFLAASLEAGEREAAEREVQRQRELAAAQKLADTEKQ
                     RAEEQTHSATQLRKRAYYLIGAFVIALIMAIAAIFLSRQAQISSRLATTRELASASIS
                     NLDVDPERSILLALRAVNKTYMVDGTVLPEAEDALHRAVQASRIELTLRGHTDEVWGA
                     VFSPDGTRIATSSADGTAKIWDANTGKDLLTLKGPVPGVVWHTMFSPDGKLLATAGQD
                     GIARIWNVTTGEQVLGLAGHTEDIFHIEFSPDESRLATISRDGTAKVWDALTGKELLT
                     LDQGADAVPYWVAFSPDSSRIAVANTRGGFEGWVSIWDVVTGEMLLALPDQNAHVNSA
                     SFSPDGTQLLTTSDDQTTRIWDANSGEELKTFYGQTINITNAAYSPDGTRIATAIGNS
                     QISILDAVTGRELLTLAGHSSDIRTVAFSPDGKRMVTASRDNTVRVWNVGPSQEFLTL
                     VNGPAILSQIGAKLAYSPDGTRLAVAYSDTIAKVWELTTGKLLFNLDGHSDAVVFIAY
                     SFDSTRIATAAMDGTAKVWDAKTGKELLTFSGHEDVVAGVAFSPDGSRLATASFDETA
                     ILWDTKTGEEIFKLTGQADWLVGVAFNSQGSRFATSSFGGTAMIWDTMTAKRLFGLLG
                     HTDTVRQVVFSPDDTRLATAGLEGNAKLWDANNGKELLTLSGHAGQVFSIAFSPDGKT
                     VATASGDRTAKLWNPLTGNALLTLHAPDGLTSVAFSPDGSQLAVASRDGTARVYLLRI
                     EDLMTLARQRVTRELTTEECQQYLHMEQCPLEP"
BASE COUNT         1405 a         1570 c         1474 g         1526 t
ORIGIN      
        1 caggtgaaac cacaaatcgc tgtagcaaat gcaaggatgg agaaatcgaa atcaaaagct
       61 gtggctcatc agggttcgcg tcgctcgaag accaggcggt cgaaataaaa tggtttgtcc
      121 ccgcttgaga ttcgcctgcc aatcaaatac aggctgatat aagggtcaag ctgccgccct
      181 ttctagactt acgatctttt tatttagtca cggacaaact ttgcccatct ggcctgtagg
      241 tcaaattctt tcaccttcca gccattgttg agccaggctg tgcgatgact ctggatcttg
      301 ggctggacat ttgtccacca ggtggccaat ttggttgcgg attttggtaa cgggtttcct
      361 aagatttctt ccaccttgtc aaaggttaac ttgatttgca ttttggaggc aggttgttta
      421 cgaaaccaat tctccaacgg caggtatttt gtaaatgcag gttgctttgc cattcaggat
      481 ttctccgaga gatgaattca ctggttggct tgaattatag aactgcttca acaaatactc
      541 aataaccccg tacagaccat gtctttagga atccggatgt aaagttttta ctgctgaata
      601 aactttagta tcctatcgag aacgacttca ggggcttcgc gatgcacaaa gtggcccgcc
      661 tctggaatgc ggaccagttc atataccgca gtaaaataat tacgcgtttg ctcgatattt
      721 ttgttatcca gggcgccatc caggtctccg aatatcgaca acgttggaac cgcggtcctg
      781 gcttggagaa ctcgccgaac cctgacgcct cttggtccca ttgcatcccc ggcaacgctc
      841 cagtaatagc ccagcgcgtg ctcgactccg tgcggtgctg ccagtgcttg cttgacaggt
      901 tcgatttctg caggcattac cgcccaggca ggtgaccatc gccgatagat cgcttcgacc
      961 tctgcgaagt tatttcgttt gagccgtgca actatccatt tgcggaactg gaatgtgata
     1021 aagtgatgtg cctttagtag agtacgaagg ttggctcgga gcgtcccggg atgagggata
     1081 gcaatcgtaa ttaacttact gatgcgctgt gagtcaatat ttgctgctgc ataagctgcg
     1141 aacgcacccc agtcgtgacc gatcacgatg gcctgttttt ctcccagcgc ctcgatcaag
     1201 gccaaaacat cctgccctaa acttagggca gaatagtcat tatttgttgg tgccgcgcta
     1261 ggggaataac ctcgcatgaa cggcgccacc acacggtagc cagcttccac taaccgcggc
     1321 tgaacctgga accaggtatg gggcgtatct ggaaaaccgt gtagcaacag gataagggga
     1381 ccgcttccct cctcatagta tgcaaatgct agtccgttcg cggtaatgta cttaattact
     1441 tgcgtcattg aacattctcc aacttaatct atgggaaatt ttacttctga tttgaaaagg
     1501 cttatccatc acagataaaa caaaacgccc cgcgttcaga gcgaggcgtt gctaggttga
     1561 agttgggatt agtggcgcct gttgtatttt ttcacggcaa tatcataagc ctcggcaagc
     1621 aacggcttta ttgtttcaaa tgtcgcttcg ctcagcgaaa gcacacaaat aaacaactgc
     1681 gcggaataat cagggtgggg catgatgaga tcaagtgcgg tgaagtcata gtcgctgacg
     1741 ttgatcttgt ctgttccaaa tagcgattgg aatgtctccc tgcttacacc gatattcaat
     1801 cgatacactc ctgggcggtc aagccttgaa accttctcat actcattacc tgacgatgca
     1861 attgttgcaa atggaaccat gtggtcatcg cgatagaaaa agaatacata tcctaaattc
     1921 gttgagtttt cgacactttc aaatgtgctg gtgatatagc tcgagataga tgattcgtct
     1981 atcacggatc cttcctttct tcactgtgaa tggattgttg accaataacg aatcgctact
     2041 atatcacaag gctctataaa tcagcgctat ttaaggttcg agtgggcatt gctccatatg
     2101 caaatactgc tggcattcct cggttgtcag ctctcttgtg acacgctgcc tggcgagcgt
     2161 catcaaatct tcgatcctca gtagataaac gcgcgcagtc ccgtcacggc tagcaactgc
     2221 tagttgggag ccatctgggc tgaaggccac gcttgtgaga ccatcaggag cgtgaagtgt
     2281 aagcagtgcg ttaccagtca gtgggttcca gagttttgcg gttctatctc cactggcggt
     2341 agcgacagtt ttgccatcgg ggctaaaggc aatgctaaac acttggcctg catgacctga
     2401 taaagtcaat aattccttac cattattagc atcccacaat ttggcgttgc cttccaaacc
     2461 ggccgttgcc aggcgtgtat catccggact aaatacaacc tgccgaacgg tatccgtatg
     2521 gccaagtagg ccaaagagtc tttttgcggt catggtatcc catatcattg ctgtgccgcc
     2581 aaaactggag gttgcgaatc gggagccctg tgaattgaag gctactccga ccagccaatc
     2641 tgcttggccc gtaagcttga agatttcctc gccagttttt gtatcccaca agatcgcggt
     2701 ctcatcaaag cttgctgttg ccagacgaga tccatctgga ctaaaagcca cgccagccac
     2761 tacatcttca tgaccagaga atgtcaacaa ttccttcccg gttttggcat cccacacttt
     2821 tgcggtccca tccattgcag cagttgcaat gcgagtgctg tcaaagctat aagcaataaa
     2881 aaccacggca tctgagtgtc catctagatt gaataatagc ttgccagttg ttagctccca
     2941 caccttggca attgtgtcag aataagcaac tgccagtcga gttccatccg ggctgtaggc
     3001 aagtttggct ccgatctgcg ataggatcgc aggtccattc accagggtga ggaattcttg
     3061 gctcggccca acattccaaa ctcttacagt gttgtcacgg ctggcagtga ccatccgttt
     3121 tccatcgggg ctgaaagcca cagtgcgaat atcgctgcta tgtccggcca acgttaataa
     3181 ttcccgacct gtgaccgcat ccagaatgct aatctggctg ttcccaatgg cggttgcgat
     3241 ccgtgtaccg tcaggactgt aggcagcatt cgtgatatta atggtttgac cgtagaaggt
     3301 ttttagttct tctccgctat tggcgtccca tattctcgtg gtttgatcat cgcttgttgt
     3361 gagaagttgt gtaccatcgg ggctgaacga cgcactgttc acatgcgcgt tttgatcagg
     3421 aagagcaaga agcatttctc ctgtaacgac atcccaaatg ctgacccatc cttcaaaacc
     3481 tcctcttgta ttcgcaacag caatgcgtga gctatctgga ctgaaagcaa cccaatatgg
     3541 cactgcgtcg gctccctggt ctagagttag taattccttg ccagtgagag catcccacac
     3601 ttttgcagtt ccatcgcggc tgatggtagc cagccggctt tcatctgggc tgaactcaat
     3661 atggaaaata tcttcagtgt gacccgccag acccaacact tgctctccag ttgtcacatt
     3721 ccagatgcga gcaattccat cttgtccagc ggtcgccaat aattttccgt ccgggctaaa
     3781 catcgtatgc catactacgc cgggaacagg acctttcaag gttaggaggt ctttgccagt
     3841 gtttgcgtcc caaatctttg ctgttccatc ggcgctggac gtcgcaattc gtgtgccatc
     3901 tggactgaac actgcgcccc aaacttcgtc tgtatgacct cgcaaggtca actcaatccg
     3961 cgaagcctgt acagcgcggt gcagtgcatc ctctgcttct ggcaagactg tgccatcgac
     4021 catgtatgtt ttattcacag cccgtaatgc caacaagatg ctacgctccg ggtccacatc
     4081 caaattgctg atggatgccg atgccaattc acgcgtcgtt gctaggcggc tggaaatctg
     4141 cgcctgcctg ctcaagaaga tcgcggcgat tgccatgatc aaggcaatga cgaatgcacc
     4201 tataagataa taggcgcgct tgcgtaattg tgttgcggaa tgtgtttgtt cttcggcgcg
     4261 ttgcttttcg gtatcggcga gtttttgcgc tgctgctaat tcgcgttgac gctgaacctc
     4321 acgctctgcg gcttctcgtt cgccggcttc taacgaagca gccaggaact caacttccaa
     4381 agcattcatt tcttcttcat gagttgagga ccattcgctt acttgaacaa gccgtgcacc
     4441 acgatatagc aaatcgggat cgtgattcat cgcgacccaa tcctgtgcgg cttcggtcaa
     4501 ttgcctgtgc agacggagcc catcgcgatt ttcttccagc cagccaagca gggtgggcca
     4561 ttcgcgaatc agtgcttcgt gagccacttc cgctgcacct tctgtcgttg tgatgagacg
     4621 ggcatcggcg agtgctttga gaacaacgtg cgttgttgct gcttcttcag gcttcaagat
     4681 caactcatca aaagtagcac gccgacgagt atcgcctgcc ccggcttcat cgcccaattc
     4741 cgttaagcgc agaaagattc gccgggcgat cgcctgctgt tcatgcgtga actgatcaac
     4801 gaagacagct tccgcggttt cagcaatggc gccacgcaca ccgcctgagg atgtgtaccc
     4861 actgagggtc atcgtgcgcc cgcgccgtct ctgccaggtt tcgaataagg catgcgagag
     4921 caatggcagc gcgccgggtt cacgaccgac gtcatgcaat aacagatcaa ccagaccgtg
     4981 ctcaaactcc caacgaccgc gctgggctgg ttcttcaatg gctcgcctta attcttcctc
     5041 attcatcgcg ccgatatatt cctggtgtgc ggcaagcgcc tcgcgcaaac gaggatagtt
     5101 cgcacaggga gcataaaagt cggcgcgaag tgttatgaca acaaggttag gaccatccac
     5161 ttctgaggca gctgtgagca ggttgccaat aaatgcagcc ctttcctctt ccgaacgaca
     5221 caaggcgaac aactcctcaa actggtcaac gaccagcatg agccgtgagc cattcataga
     5281 tctcaggatc cgcttggtaa agatttgcag gctccgctcg tcgcgcgcca gatcatccat
     5341 gagtgttgcc gtcgcgatga cagagttact ttcctgcgtt aatgttccgg ccaggctctc
     5401 taacggatgc gcggtgggcg tcaggatgtg aatccgccaa tcagtggaag ccttattcca
     5461 acgtaatgcg gaaacgagcc ccgcgcgtgc aagcgaggac ttaccgctcc ccgacgcgcc
     5521 gacaaccgcc agaaaacgcg tcttgtgtga ggaccgggtg gaggtcaaag caaggacacg
     5581 ttctataagt ttcgcagtga gtgtttcacg tccgacaaaa aaatcagcat cggtttcgtc
     5641 aaagtagttc aaacctttgt agggagataa tccggtcgcg ggcgcatctt cacgcctcac
     5701 attggcagca agatccagca gtcgcgcgac tgcttttggt tcctcctcca gcccaagcgc
     5761 ggaaacaaag cgcgcctcga tcgtggggat atccggcagt cgctcgttct gttcgaggcg
     5821 gctgatctgg gtgttgctat aaccgaccgc gatcgacagt tccatctgtg tgatacccat
     5881 gcgccttcgc agaaagcgca gcaagtcacc aaaggttatg aacttatcga gtgtgctggc
     5941 agggatagaa gtggccatgc tgtttcctct gtaat
//
LOCUS       DASTJP010000811         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_709360, whole genome shotgun sequence.
ACCESSION   DASTJP010000811 DASTJP010000000
VERSION     DASTJP010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709360"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFQ23_23705"
     CDS             <1..281
                     /locus_tag="VFQ23_23705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909673.1"
                     /translation="TDRIRDVFIIVVALESLVIGVALIILIVQLASLINLLQNEVRPI
                     LAATNETVNHLRGTAEFLGENVVEPVIKLNGYLAGMNRVIELMGIKKK"
     gene            399..758
                     /locus_tag="VFQ23_23710"
     CDS             399..758
                     /locus_tag="VFQ23_23710"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9909674.1"
                     /translation="MSDRDEFGAFLVGFIVGGLSGAVVALLFAPQSGEETRALIKDKS
                     IELRDKAQITAEEAIARAEAAAADARARADDLARQLRERGHVVVEDVRERGKSAVDAV
                     RSKVGRKSDETSESTAM"
     gene            836..1528
                     /locus_tag="VFQ23_23715"
     CDS             836..1528
                     /locus_tag="VFQ23_23715"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9909675.1"
                     /translation="MKTLLRFFFRLLYHQFAVTYDVVAATVSLGRWKDWVLSVVPFIQ
                     GNRILEIGHGPGHLQRLLLSRNLLAFGIDESTQMGLLAKRNLMRSLPSEADKSKPHRN
                     AHAQLNLTRGISQYLPFLPESFDTLVATFPSEYIFDPRTLAEAHRALTPKGRFVILPG
                     ATIMGRGVMDRAMALLFRITGQTPPDLSEILYERSKEHFAKAGFSTEVHELHIKSSLV
                     YILVATKSSNSK"
     gene            1539..2177
                     /locus_tag="VFQ23_23720"
     CDS             1539..2177
                     /locus_tag="VFQ23_23720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET9909676.1"
                     /translation="MLKKLREPVNSLTHWGGAFLALIGLVALLVVGWGTPAKVISLLV
                     YGVSLIFLFSASATYHMVQVKDKALEIFRKIDHAAIYCLIAGTYTPFCVNAFSGFWKW
                     GMLSIIWSLALTGIIVKVFYIRAPRWLNAGIYLIMGWLCIAAIGQMLAALPVWVLIWL
                     IAGGLIYTFGAVVYITKIFNFVPGVFGFHEMWHILVMLAAAAHFVAVLGVAL"
     gene            complement(2271..>2779)
                     /locus_tag="VFQ23_23725"
     CDS             complement(2271..>2779)
                     /locus_tag="VFQ23_23725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909677.1"
                     /translation="VTELAQGQENYFSFLSDSRANITTVLGDARISLERELAEGNPQN
                     FDVLVLDTFSSDSIPVHLLTKEAFDVYLAHLAPDGIVAAHITNLHLDLQPVFWQFSQH
                     YGLSMARINYDGDSNGGSASHWILLSRDPALLEVPAVQVRSVDLNGYFTDLKLWTDDY
                     SNLFQILK"
BASE COUNT          656 a          711 c          735 g          677 t
ORIGIN      
        1 ccacagaccg catccgcgat gtctttatta ttgttgtggc gcttgaatcc ctggtgatcg
       61 gcgttgcctt gatcatcctg atcgttcagc ttgcaagcct gatcaacctg ctgcaaaatg
      121 aagtccgccc gatcctcgcc gccaccaacg agaccgttaa tcacctgcgc ggcacggcag
      181 agttcctcgg agagaatgtc gtcgagccgg tgatcaaact taatggctat ctcgccggga
      241 tgaaccgcgt gatagaatta atgggcatca agaagaaata acaaaagcaa atcagagcag
      301 tctacgagtt tgccagacgt ccctacaacg tctgcaaaat tcgagataaa attcacactc
      361 gcaatttcaa atcgtttgat aaccagaagg aggttactat gtctgaccgt gatgagtttg
      421 gcgcttttct cgtggggttc attgttggcg ggctttcggg agccgtggtt gcactgctct
      481 ttgcaccgca atccggcgag gagacccgcg ccttgatcaa agacaaatcc attgagctgc
      541 gcgacaaggc ccagatcacc gcagaagaag ccattgcccg cgccgaagcc gccgcggccg
      601 atgcccgtgc ccgagccgat gatctcgcga ggcagctgcg cgaacgcgga catgtggttg
      661 tggaagatgt tcgtgagcgc ggcaagagcg cagtcgatgc cgttcgcagc aaggttggca
      721 ggaaatcgga cgagactagc gaatccaccg cgatgtaagt attattcaac aaggcaaggg
      781 tttgactcgc ggtgcggtca aacccttatt ttttatccac aggaccacgt cgcttgtgaa
      841 aacactgctg cgcttcttct tccgcctgct ctatcaccag tttgcggtta catacgatgt
      901 ggtcgcggcg acggtttcat taggaaggtg gaaagactgg gtcttgagcg tggttccctt
      961 tatccagggg aaccggatcc ttgaaattgg tcatggaccc ggacatttgc agcggctcct
     1021 tctcagccga aacctgttgg cctttgggat cgacgaatcc acccagatgg gtcttctcgc
     1081 caaacgcaat ctgatgcgct ccctgccttc tgaagcggac aagtcgaaac cccaccgcaa
     1141 cgcccacgcg caactcaatt tgacacgcgg gatatcccag tatctccctt ttctaccaga
     1201 gtcgttcgac acgctggtag ctaccttccc atcggaatat attttcgacc ccagaacgct
     1261 cgctgaggct catcgcgcgt taacgcccaa agggcgtttt gttatcttgc ccggcgcgac
     1321 aattatggga cgcggcgtca tggaccgagc catggcatta ttgttccgta tcaccggaca
     1381 gactcctccg gacctttcag agattctcta tgagagatca aaggagcatt ttgccaaagc
     1441 tggtttctca acggaagttc atgaattgca cataaaatca agcctggttt acatcctggt
     1501 agcaacaaag tcttcgaatt ccaagtaaag gattggtcat gcttaaaaaa ctccgagagc
     1561 cggtcaatag cctcacgcat tggggaggcg catttcttgc cttgatcggc ttggtcgcct
     1621 tgctcgttgt cggatgggga acgcctgcca aagtgatatc actgttggtc tacggtgtca
     1681 gcctgatctt tttattttcg gcaagtgcaa cctatcacat ggtacaggtg aaggacaaag
     1741 cgttggaaat cttccgtaag attgaccacg ctgcgatcta ttgcctcatc gccgggacgt
     1801 atacgccatt ttgcgtaaac gcgttttcag gcttttggaa atggggcatg ttgagcatca
     1861 tctggtcact ggcgttgacc ggaatcattg tgaaagtgtt ctacattcgc gcaccgcgct
     1921 ggttgaacgc ggggatttat ctaatcatgg gctggctttg catcgccgct atcgggcaga
     1981 tgcttgctgc cctgcctgtg tgggtcctga tctggttaat cgcgggcggc ttgatttaca
     2041 catttggcgc ggtcgtatac atcacgaaaa tcttcaattt cgtcccgggt gtatttggat
     2101 tccatgaaat gtggcacatc ctggttatgc ttgccgccgc cgctcatttt gtcgcagtgc
     2161 taggggtggc actgtaaatt gtcacgctga aggcggtcgt tgcaaagcga tcctgatatg
     2221 cctgcgaagt ttcggggaag ttttttcacg aagaggatta cacgatcgcg tcacttcaag
     2281 atttgaaaca agttgctgta atcgtcggtc cacagtttga gatcggtgaa atagccattt
     2341 aaatcaactg aacgcacctg aacggcagga acttcgagta aagcaggatc acgagaaagc
     2401 aaaatccagt gagaggcaga gccgccgttt gaatccccat cgtaattgat tcgggccata
     2461 ctcaagccat aatgctgcga aaattgccag aagacaggct gcagatccag atgcagattt
     2521 gtgatatgcg cggcaacgat tccgtcgggg gcaagatggg cgagatacac atcgaaggct
     2581 tccttcgtca acaaatggac ggggatcgaa tcactgctaa atgtatcgag aaccagcaca
     2641 tcaaagttct gagggtttcc ttccgcaagt tcgcgttcga gtgagatgcg cgcgtcgccg
     2701 agaacagttg tgatattcgc ccggctgtcg ctgagaaacg aaaagtaatt ctcctgcccc
     2761 tgggcaagct cagtcacaa
//
LOCUS       DASTJP010000812         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1238187, whole genome shotgun sequence.
ACCESSION   DASTJP010000812 DASTJP010000000
VERSION     DASTJP010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238187"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFQ23_23730"
     CDS             <1..482
                     /locus_tag="VFQ23_23730"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9909678.1"
                     /translation="IKAQLAPLYRQGFERMIGGPPEQIFADVLKTDTPMAKTVRSGLY
                     LVNHFAQVPLFLIGFGRTKDGAGIYPALQNAMLAARAEGIGSTLTGFLQSYFANEVMD
                     ILGVPKDDGWYMHGVVPMGYPLGKWGFGARKPVHEVAARNSWQGDLGFTVPESLWQK"
     gene            470..742
                     /locus_tag="VFQ23_23735"
     CDS             470..742
                     /locus_tag="VFQ23_23735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909679.1"
                     /translation="MAEVIGSDQPMIYQIKVKSHLSSNWTDWFDGLTITPEVDGDTLL
                     TGPVIDQAALYGLLKKVRDLGMILISLNPVQFNHASPEKGEQKNEV"
     gene            732..1319
                     /locus_tag="VFQ23_23740"
     CDS             732..1319
                     /locus_tag="VFQ23_23740"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HET9909680.1"
                     /translation="MKSNPAREIQITAHSNTSQLLYARVAGVTFLYYIAAGITSLAVA
                     AQSPIADVLTLSTSFAAIVLGITLYAITREQGPALALLAMMCRVVESIHAESAIFFAV
                     GSLIFSWLLLRGRMIPIVLAWLGVIASALLVVILPLQLAGLFGGATNWSQSADWLTWI
                     PMLVFEVALAFWLVIKGVAVQSGRTTTSLGADPLL"
     gene            1358..2071
                     /locus_tag="VFQ23_23745"
     CDS             1358..2071
                     /locus_tag="VFQ23_23745"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9909681.1"
                     /translation="MINRTKEISPQFYARLIAVLYLIVIAAGITAQMGISEGLVVAGD
                     AAATANNILAQKDLFQLGFTIYLIEMICQIAQMALFYILLRPVNRNVALLSLVFSLIG
                     CTIKTLGRLFYISPLLVLGDSTYLNVFNDGQLEALALLLLNVNEQAAGIAVAFFGVST
                     FLNGYLVFKSTFLPSWLGVLSMVGGLGWFTYVYPPLGNQLSLYILFFALIGSLAEIGW
                     LLIKGVNVEQWNKRALEAA"
     gene            2129..2797
                     /locus_tag="VFQ23_23750"
     CDS             2129..2797
                     /locus_tag="VFQ23_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909682.1"
                     /translation="MVLLPIHIIAGLTGIVSGFIALYTLKGGKLHRKGGMIFVYAMLV
                     VAITGVVMGALISEMSAVIPGVLAFYLVMTALRSIRHPVLKFHWPDLGLLLLALILGG
                     GSILYGILAEDQPTALYIVFGIVTLLAALGDVRVMLAGSIQGKQRVVRHLWRMGLAMF
                     IATGSFFLGQSDEFPEQFRNSGLLSIPVLLVIFTMFYWLVRVSFTQWYRRFQQQLQAD
                     ISIS"
     gene            3039..>3511
                     /locus_tag="VFQ23_23755"
     CDS             3039..>3511
                     /locus_tag="VFQ23_23755"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9909683.1"
                     /translation="MKAIKYYEYGPPDVLTIEEVDKPTPKDDQILIKVHAVSINGSDR
                     EGLIGKPLYARIGGLRKPGEQILGSDIAGRVEAVGKSHTEFQVGDEVFGELPNYHGGF
                     AEYVCTHGRTLAKKPADLTFAEASAIPQAGVIAIRAIRDKGQVQPGQRVLMNGAGG"
BASE COUNT          805 a          899 c          918 g          889 t
ORIGIN      
        1 agatcaaggc tcagctcgcg ccgctctatc ggcaaggatt cgagcgcatg atcggcggac
       61 caccggaaca gatcttcgcg gacgttttga agacggatac gccaatggcc aaaacggttc
      121 ggtcggggct gtacctggtg aatcactttg cccaggtgcc cctattcctc atcggatttg
      181 gtcgtacaaa agacggtgca ggtatctacc cggccctcca aaacgcaatg ctggctgccc
      241 gcgccgaggg catcggctct acgctgactg gcttcctaca gagttacttt gccaacgagg
      301 tcatggatat tcttggtgtg ccaaaggacg acggctggta catgcacggt gtcgttccga
      361 tgggctaccc gctcggaaag tggggcttcg gggctcgtaa accggtccat gaggtggcgg
      421 cgcgcaatag ttggcagggt gacctgggat tcacagtgcc ggagtcatta tggcagaagt
      481 aattggttca gatcaaccaa tgatttatca aatcaaggtc aagagccatt tgagctccaa
      541 ttggaccgat tggtttgatg ggctaacgat cactccggaa gtggatggcg atacgctgtt
      601 aaccggtcct gtgatcgatc aagcagcgct gtacggactg cttaaaaaag tacgggattt
      661 gggaatgatc ttgatctccc ttaatccggt tcagttcaat cacgcttcac ctgagaaagg
      721 cgagcaaaaa aatgaagtct aaccccgcaa gagaaattca aatcaccgcc catagcaata
      781 cctcgcaact tctatatgcc agagtcgctg gagtcacatt cctctactat attgctgccg
      841 ggatcaccag tttggccgtc gcagcacagt cgcctatcgc agatgtactc actttgtcca
      901 cctcttttgc ggccatcgtt ctcggtatca cgctctacgc gatcacgcgc gagcaggggc
      961 ccgctctggc cttacttgca atgatgtgtc gcgtcgttga gtccattcat gccgagtctg
     1021 caatcttctt cgctgtgggc agtctgattt tttcctggct tctcctgcgc ggccggatga
     1081 tccccatcgt actagcctgg ctgggtgtga ttgcatctgc tctgctcgtc gtcatccttc
     1141 ctttgcaact cgccgggctc tttggagggg ctacaaactg gtcgcaatcg gccgactggc
     1201 ttacatggat acccatgctg gtgttcgaag tcgctctggc cttttggctg gttattaagg
     1261 gcgtcgctgt tcaatctgga cgtaccacca cttcactcgg agcagaccca ctgctatgag
     1321 tgaaaattta ttcgcttcaa acttgaggag taatcaaatg atcaatcgaa caaaagaaat
     1381 atcaccgcag ttttatgcgc gccttatcgc ggtgctttat ctgatcgtca tcgccgcagg
     1441 catcactgcc cagatgggaa tcagcgaagg actagtcgtc gctggggatg cggcagcgac
     1501 ggcaaacaac attctggcgc aaaaggatct ctttcaactg ggattcacca tctacctgat
     1561 tgagatgatc tgtcagatcg cccagatggc tctcttttat atcctgctta ggcccgtaaa
     1621 cagaaacgtc gcattgcttt cattggtctt cagtctgatc ggctgcacca taaaaaccct
     1681 gggccgcctg ttttatatct ctcccttgct cgtcctgggt gactcgactt acctgaatgt
     1741 tttcaacgat ggtcagctgg aggcactagc actcctgttg ctcaacgtga atgaacaggc
     1801 tgcaggcatc gcagtggcgt tcttcggggt ttccacattc ctgaacggct atctggtttt
     1861 caaatccacc tttctgccca gttggttggg agtgttatcc atggtgggag gtttgggttg
     1921 gttcacctac gtataccccc cactgggaaa ccaattatcc ctttatattc tgttcttcgc
     1981 tctcatcgga agcctggcag agatcggctg gctgctcatc aagggcgtca acgttgagca
     2041 atggaataaa cgtgctttag aagcggcttg aatgaaatca actcggcaat cgaagtgccg
     2101 gtcgatcgct acagaaaagg aaaatcatat ggtgttacta cctattcaca ttatcgcggg
     2161 tttaactgga atcgtctccg gattcatcgc actttatact ctgaagggag gaaagctgca
     2221 tcgtaagggc ggaatgatct ttgtctatgc catgctggtt gtggcaatca ccggcgtcgt
     2281 gatgggggca ttaatctccg aaatgtctgc agtaattccc ggtgtattgg cgttctatct
     2341 ggtaatgacc gcattgcgtt cgatccgcca cccggtccta aaatttcatt ggccagatct
     2401 ggggctcctc ttgctggcct tgatcctggg cggtggcagt attctctatg ggatcctcgc
     2461 cgaagatcag ccaaccgccc tgtatattgt ctttggcatc gtgacgttgc tcgctgcttt
     2521 gggggacgtt cgtgtaatgc tggctggtag tattcagggc aagcagcgtg ttgtgagaca
     2581 cttatggcgc atgggactgg ccatgttcat tgctaccggt tcgttctttc tcggccagtc
     2641 agacgagttt ccagagcagt ttcggaactc tggactgctg tcaatcccgg tcctgctggt
     2701 gatatttacc atgttctact ggctggtacg agtatcgttc acgcaatggt atcgacgctt
     2761 tcagcaacaa cttcaggcgg acatttctat ttcctgacgc agtcatctac gtcaagaact
     2821 tgtagagagt agctaaactc aaggaaatgg agaacacatg tcaccctgag catagcgaag
     2881 ggtctccgcc gctgcgcggc gggattcttc actccctagg gtcgttcaga atgacaaaat
     2941 catttgtaag ttaagcactc ccaacttata aacctttaca gcggattatc gtatactcag
     3001 taaacgtccg agaaaaaatt actcctggag gagtagggat gaaagccatt aaatattacg
     3061 aatacggtcc accagacgtc ctgaccatcg aagaagtgga caaacccact ccgaaggatg
     3121 accagatcct catcaaggtc catgcggtat ccatcaacgg ctcagacaga gaaggactga
     3181 tcggcaaacc gttatatgca cgcatcggtg gtcttcgaaa gccaggtgaa cagatactcg
     3241 gctcagacat tgccggtcga gtggaagcag taggcaaaag ccatacagaa ttccaggtag
     3301 gggatgaagt atttggagaa ctcccaaact atcatggtgg ttttgctgag tacgtctgca
     3361 cccatggcag gaccctggca aagaaacctg ccgatctgac atttgcagaa gcctcggcca
     3421 tcccacaggc tggagtgatc gccatacgcg cgattcgtga caaaggccag gtgcagccag
     3481 ggcagagagt cctgatgaac ggcgcgggcg g
//
LOCUS       DASTJP010000813         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_288882, whole genome shotgun sequence.
ACCESSION   DASTJP010000813 DASTJP010000000
VERSION     DASTJP010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288882"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /gene="secD"
                     /locus_tag="VFQ23_23760"
     CDS             <1..984
                     /gene="secD"
                     /locus_tag="VFQ23_23760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET9909684.1"
                     /translation="FVDTGDYSPDPGTVLKTDFVTGSDTLPTPEGTDPAQDVVYHTIM
                     TGQVLDSVNVSAGQVGGAAVDFVLTPEGRTIFAQHTAANTNKYLTITLDKVVVSSPII
                     QNAIPDGLGQISGGFTPEGANALAVQLRYGSLPIPLKVVETRIIGPTLGEDSLQKSLL
                     AGMIGMIIVILFMVIYYRLPGIVAALSILFYAAISYAIFKYFHFTLTLPGIAGFLLST
                     GAALDANILIFERLKEELRNGRSLTQAVDQGWTRAWSSIRDSNLSAIITSLILYWFGS
                     NFGATIVKGFSLTLALGVAISLFTAIYVSRTLLTAALQNFKPKNLEFWFGL"
     gene            999..1922
                     /gene="secF"
                     /locus_tag="VFQ23_23765"
     CDS             999..1922
                     /gene="secF"
                     /locus_tag="VFQ23_23765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET9909685.1"
                     /translation="MIDILSKRYLYFAISLLVILPGLIVLGSRGLPLSIDFTGGSLLE
                     VQFEGGNSLQNAEIVQLYENADIADAQVQTTDSGTLLIRSSFLSNETRDLILNEMREQ
                     SGGEVTVIRFDSVGPSIGAAVASRAALAVGVAALAVVVYITWAFSRVPNAFRYGVCAV
                     IAMIHDVAVVFSVVGFGALFLNWQIDSLFLTALLTVIGFSVQDKVVVFDRIRENTNIF
                     RRLDYEKLVNHSIVQTLQRSINTQLMTVEFLLLALALFGGITLQEFAVILLVGLFSGT
                     YSSIFIAAPILVLWEKREWETWFRRPQQSGA"
     gene            complement(2367..3620)
                     /locus_tag="VFQ23_23770"
     CDS             complement(2367..3620)
                     /locus_tag="VFQ23_23770"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET9909686.1"
                     /translation="MTLENTTISFFDRPSIEMGTVDVADGIPFGIERVTNLSVGIGGV
                     YGTWGRSYDNETLHFFLEERLGRPLQPNEKLNLSELGFRNRHHVPLSLTPEEHDKLEV
                     EIGTRFLREAINACNWQPSEVEAVLIGMSAPLSEDYLDRITQAAGIPESALKVSIHKA
                     CDGSTSSLHLALNPDLPENSQLDQNIAEYLYGKKVLVGGIEGLSRFIVSSHDANALQL
                     FGNAAGVVGVIPGKTMKFLAGRSREVFDDEGVLQVAMYYPHSGQKTDGSYNVDVTEPL
                     ENNIRVAGLMHEPEDGSSIQMAGPMGMVKLFVRTGVQVVRDVYGAYQSKLDELGMAGK
                     SLAVAIVHHANYKINKLKEKHLQNEGIVLPMPWLLSEFGNVSAASNMIAFLRELPNMK
                     PLDHILIDGFGAGTYYDALAVELGG"
BASE COUNT          924 a         1044 c          947 g         1052 t
ORIGIN      
        1 tttgtggata ccggcgacta tagccctgac cctggaactg ttctgaaaac agattttgtg
       61 accggttcag ataccttacc cacacccgaa gggaccgatc ccgcgcagga tgtggtgtat
      121 cacacaatca tgaccggcca agtgcttgat agtgtcaacg taagcgcagg gcaagtaggt
      181 ggcgctgcag ttgattttgt gctcacccct gaaggaagaa ccatctttgc ccaacatact
      241 gccgcgaata caaacaaata cctgactatc accctggata aggttgtggt ttcatcacct
      301 attattcaga atgctatacc agatgggcta gggcaaatta gtggaggatt tactccagaa
      361 ggagccaatg cgctggctgt gcagttacgc tacggttcac ttcccattcc cctcaaagtc
      421 gttgagacac gcatcatcgg tccgacattg ggtgaagatt ctctgcaaaa gagtctgctg
      481 gccgggatga tcggtatgat catcgtgatt cttttcatgg tgatttatta tcgtctgcca
      541 ggtatcgttg cggctttatc catcctgttt tatgcagcga tttcctatgc catctttaaa
      601 tacttccatt tcacactgac gcttcccggc attgcaggct tcctgctcag cacaggcgcg
      661 gcattggatg cgaacattct gatctttgag cgcttgaagg aagagcttcg caacgggcgg
      721 agtcttacgc aagcagtcga tcagggctgg acccgtgcct ggtcttccat ccgtgactca
      781 aacctctcag ctatcatcac ctccctgatt ctctactggt tcggatccaa ctttggtgca
      841 accatcgtaa agggtttttc tctcacgctg gccctgggtg ttgctatttc gctatttact
      901 gctatttatg tctcccgtac gcttcttacg gctgcattac aaaacttcaa accgaaaaac
      961 cttgagttct ggtttggact ctaaaggacg aggaacccat gattgatatc ctcagcaaac
     1021 gttacttata ttttgcaatc tctcttctgg tgattctgcc gggtctgatc gtccttggca
     1081 gtcgcggatt gcccctctcc attgatttca ctggcggctc cctgctggaa gtgcaattcg
     1141 aaggcggaaa ttcgttgcag aacgcggaga tcgttcagct ttacgaaaac gccgacatcg
     1201 cagacgccca ggtccaaacc acggacagtg gtacgttgtt gatccgttct tcttttctct
     1261 ccaacgaaac gcgcgacctc attctcaatg aaatgcgaga gcaaagtggt ggcgaagtca
     1321 ctgtcatacg ttttgatagc gtaggaccaa gcattggcgc agccgtggca tcccgtgcgg
     1381 cactggcagt tggcgtggcc gctttagccg tggtagttta catcacctgg gcatttagcc
     1441 gagttccgaa tgccttccgt tacggcgtat gcgcggtgat cgccatgatt catgatgtgg
     1501 cagttgtgtt cagtgtagtt ggctttggcg cgctgttcct aaactggcag atcgactcgc
     1561 tcttcctaac cgctctgctg accgtcatcg gattttcggt gcaggataaa gtggtcgtgt
     1621 ttgatcgtat ccgtgagaac acaaacattt ttcgtcgtct tgattacgaa aagctggtca
     1681 accattccat cgttcagacg ctgcagcgtt cgatcaatac gcagcttatg acggtagaat
     1741 tccttttatt ggcgttggcc ctttttggtg gtattacgct gcaggaattt gccgtcatat
     1801 tattagtggg actcttcagc gggacctatt cgtccatttt cattgccgct cccattctcg
     1861 tgctgtggga aaagcgtgaa tgggagacct ggttccgtcg ccctcaacaa agcggcgcat
     1921 agttttggat ccctttaaat caaaagaccc tcacctatca acggtgaggg tcttttttta
     1981 taagatcatt cctaatacat tcgcatcaac gaaaaaatat agcgggttac taaagttaga
     2041 aaagaggtcc gacgtgtaga aaacatccga agaggatatt cattttcgag agactttaat
     2101 ctcagaagca acctaacaac gtcctggttt tgatttgaac gatacattac tcgcgctcac
     2161 catgtaaaaa ggaatcccaa ttgcatatac tagacgcaag ttagaaatga aagttacaga
     2221 aaacttgcaa attgcttaca aaatgtttct gcccggcgaa gttgccgggc agatggtttg
     2281 tcacacccgt actgggcgca ggcgcaagtg ttgtttcgtc gggatttcgg tgctatcgca
     2341 cctcaaccct cgtcgcaatg acagggttac cctcccaatt caaccgccag cgcatcgtaa
     2401 taggtgcccg cgccaaagcc gtcgatcaga atgtgatcca gaggcttcat atttggcaac
     2461 tcacgcagga atgcgatcat attcgaggcc gcgctcacat tgccaaattc gctcagcagc
     2521 cagggcattg gcaaaacaat cccctcgttc tgtaagtgct tctctttgag tttgttgatt
     2581 ttgtagttgg catgatggac aatcgccacc gccagcgact tccctgccat tcctaattca
     2641 tccaattttg attgataggc gccgtacaca tcccgcacga cctgcacgcc tgtccgcaca
     2701 aataatttga ccatgcccat cgggcccgcc atttgaatgg acgaaccatc ctccggctca
     2761 tgcatcaaac cagccacacg aatgttgttt tccaggggtt cggtcacatc cacgttatag
     2821 ctgccatcgg tcttctgccc cgagtgcggg tagtacatcg ccacctgaag aacaccttca
     2881 tcgtcgaata cttcgcgcga tctgcctgcc aggaatttca tcgttttgcc ggggatcaca
     2941 cccaccacgc ccgcggcatt cccaaacaac tgtaaagcgt tcgcatcgtg cgaggacaca
     3001 ataaagcgac tcaaaccttc aatgccaccg accagcacct tcttcccata caggtattcg
     3061 gcgatgttct gatccagctg gctattctca ggtagatcgg ggttcagggc taaatgcagg
     3121 ctgctggtgg aaccatcaca ggccttgtgg atggaaactt tcaaggcgct ttctgggatc
     3181 ccggcggctt gtgtgattcg atccaggtaa tcttctgaaa gaggggcgct catgccgatc
     3241 agcacggctt ccacttcaga gggctgccag ttacaggcat tgatggcttc gcggaggaat
     3301 cttgtgccga tctcaacttc gagtttgtcg tgttcctcgg gtgtcagact caagggaaca
     3361 tggtgtcggt tgcgaaaacc cagttcagac agattcagtt tctcgttcgg ttgcagcggc
     3421 cgcccgaggc gctcctccag aaagaaatgc agggtttcgt tgtcatagct acgtccccag
     3481 gtgccgtata ctccaccaat ccccacagag aggtttgtca cgcgttcgat cccgaaggga
     3541 atgccatccg ccacatcgac tgtgcccatt tcgatactgg gtcgatcgaa gaaagatatt
     3601 gttgtatttt caagggtcat ttcgtacgct cctttttgct taccatccaa acccccattt
     3661 ggtttgaaag tttcgctccg ctgtgggatc tatccgacag aatattataa ctgattcctt
     3721 tcgtttgccc tgatgagaac tcacgtgggg gtggctaaga gagtcagtga aaatctactt
     3781 agccacagag agcactgagc cctttgacag aaaaaactta aaaactctgt gggctcccct
     3841 ggcgccgccc gtgatttgct cgagcaaatc aggcaggtgt gtgacttaca cactacattc
     3901 gccactcccc tcacgtagcc gctattcccc aaaacgaaca gaaatttcag agaattacag
     3961 caaggct
//
LOCUS       DASTJP010000814         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_31865, whole genome shotgun sequence.
ACCESSION   DASTJP010000814 DASTJP010000000
VERSION     DASTJP010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31865"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..407)
                     /locus_tag="VFQ23_23775"
     CDS             complement(51..407)
                     /locus_tag="VFQ23_23775"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9909687.1"
                     /translation="MKLQSSELDGGIRLIKLIGALDMTGTYAIEVEFVRQCDGDNMHL
                     IVDLSKVNYLSSIGIPMLINTAKSVSSRGGKLVLLNPQQNVSDVLDIVGIQQIIPIYT
                     DIEAAKASVTSDISSQ"
     gene            546..1904
                     /locus_tag="VFQ23_23780"
     CDS             546..1904
                     /locus_tag="VFQ23_23780"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9909688.1"
                     /translation="MTIQNPTTLKTIPGPRGLPLLGVMPEMVTDMLGLFTKTSREYGG
                     IAQFKLLNSTYFLITNPDYVKYILQDNYKNYVRGRSVETGRVLLGNGLPLIDGDFWLR
                     ERRLLQPAFHRERLAALTSTAANVIDTFIQKWETYAQTGQPLDVDEEMMRLTLTVIIK
                     SMFSADIDSKIDALSHAFNVASKFMLWRSQQMWAPPLSIPVPRNVEYNRASKVLDDTI
                     YPLIADARKNPKDDLLGMLLAMRDEETGEGMSDKQARDEVVTIFFAGHETTAASMAWG
                     FYLLSEHPEVEERVRGEIQTVLNGRIPTSADLQNLAYTQRVIQEVLRLYPAAYLFARE
                     AVKDDTIDGYPIPPKTLIFITPYITHRDARYWPDPERFDPDRFEPEQVASRPRHIYYP
                     FGEGPHVCIGNNFALMEMQLILAIALQRFRLKLVPNHPIALKPEATLRPKHGMKMTVE
                     KI"
     gene            1950..2861
                     /locus_tag="VFQ23_23785"
     CDS             1950..2861
                     /locus_tag="VFQ23_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9909689.1"
                     /translation="MKNSQLYGGIEAGGTKFVCVVGSGPNHILDEIRFPTTTPGETLG
                     KTIQFFQPFVSSGQINVIGVGCFGPLDLNPKSPTYGFITATPKPTWSNTDVRGMLQHG
                     LKVDVAFDLDVNAAGMGEATWGASKGLDSSLYLTIGTGIGGGYIKDGKPLIGLLHPEM
                     GHIRIPHDRNLDPFNGTCPFHDDCLEGLASGPAIEKRLGLTGAFIPEDDPYWELEAEY
                     IASALMNFILILSPKKIILGGGVMQREYLFPKIRRRVAELLNGYVSSKSILENIDSYI
                     VPPALGNQSGSLGAIALAMYHGEKNQS"
     gene            2899..>3088
                     /locus_tag="VFQ23_23790"
     CDS             2899..>3088
                     /locus_tag="VFQ23_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909690.1"
                     /translation="MMSTNEVPLIPRKLLFGNPDKTQARISPDGQRLSYLAPVNGVLN
                     VWVGPVNDPESAKPVTNDT"
BASE COUNT          806 a          746 c          767 g          769 t
ORIGIN      
        1 tgcggtttga gagaatgatt cggctttctg aggatgatat cgagttgaag ctattggctg
       61 ctgatatctg aggtaacgct tgcttttgca gcctctatat ccgtatagat cggaatgatc
      121 tgttggatac caacgatatc cagcacatct gacacatttt gctgcggatt gagcagcacc
      181 agtttcccac ctcggctgga aacggacttg gctgtgttga tcagcattgg aataccaata
      241 gaggaaaggt agttcacttt ggataaatcc acaattaagt gcatgttgtc accatcacat
      301 tgacggacaa actctacctc aatagcatag gtaccggtca tatctaatgc gccgataagc
      361 tttatcagtc gaatgccccc gtccaattca ctggattgga gtttcatgaa ttttgccttc
      421 caactgggat gaaatgctga ctaggatgga ttgattatac atggcatata tcctcaattc
      481 caacggaacg gtacatttgg cggataaata aaactggtaa aatttcagta tccagatacg
      541 gactcatgac aattcaaaac ccaacgaccc tcaaaaccat ccctggacct cgcggattgc
      601 cccttctcgg cgtgatgcct gagatggtca cggacatgtt ggggttgttc acaaaaacat
      661 cacgggagta tgggggcatc gctcaattca agctgcttaa ttcaacctat tttctcatta
      721 cgaatcctga ttatgtcaaa tacattcttc aggataacta caagaattac gtgcgcggcc
      781 ggagtgtgga aacaggccgc gtcctactag gaaatggact tccgctcatt gatggagatt
      841 tctggctccg agagaggcgt ctgctccaac ctgccttcca tcgcgaacga cttgcagcgc
      901 ttacttccac cgctgccaat gtgatcgata ccttcatcca aaaatgggaa acgtacgcgc
      961 agactggtca accgcttgat gtagacgagg agatgatgcg cctgacgttg acagtgatca
     1021 tcaaatccat gttcagcgcg gatattgaca gtaagattga cgccttgagt cacgcattta
     1081 acgttgcttc caaattcatg ctgtggcgaa gccaacaaat gtgggcaccg ccgttatcaa
     1141 tccccgtgcc gcgcaatgtg gaatataacc gcgcctccaa agtattggat gatacgattt
     1201 atccgctcat tgccgacgcg cggaaaaatc cgaaagatga cttgctcggc atgcttctgg
     1261 ccatgcgcga tgaagaaacc ggggaaggaa tgtctgacaa gcaggcacgg gatgaagttg
     1321 ttacgatctt ttttgccggg cacgagacga ctgctgcttc tatggcctgg ggcttttatc
     1381 tgttgtctga acaccctgaa gttgaggaac gcgtgcgggg tgaaatccaa actgtgctca
     1441 acggacgaat tccaacctct gctgatttgc agaacctagc ctatacacag cgtgtgattc
     1501 aggaagttct ccggctctac ccggcagcct atttatttgc acgtgaggca gtcaaagacg
     1561 acacaataga tggatatccc attccgccga aaactctgat tttcatcact ccctatatta
     1621 cacatcgcga tgcgagatat tggccagatc ctgagcgttt tgatcctgac cgttttgagc
     1681 ccgagcaggt cgcaagccgt ccgcgtcaca tttattatcc ctttggggag ggtccgcatg
     1741 tgtgcatcgg taacaatttc gctctgatgg aaatgcaact gatcctcgcc atcgcgctcc
     1801 aacgcttccg cttgaagctg gttccaaatc acccaatcgc actcaaaccg gaagcgacgc
     1861 tcagaccgaa gcacggtatg aaaatgaccg tagaaaaaat atagaacctt taagcaatct
     1921 ttgtttgcag cggctggaga agaaaaaaaa tgaaaaattc tcaactatac ggcggtatcg
     1981 aagcaggcgg gacaaaattt gtatgtgtcg taggtagtgg gccgaatcac attctggatg
     2041 aaattcgctt tccaacgacc actccgggtg agactctggg aaagacgatt caatttttcc
     2101 agccgtttgt ttcatcaggg cagatcaatg tcattggggt tgggtgtttt ggcccgctgg
     2161 atttgaatcc caaatccccc acgtatggat tcatcactgc cacccccaaa ccgacctgga
     2221 gcaataccga tgtgcgcggc atgttacagc acggactaaa ggtggacgtc gcgttcgatc
     2281 tggatgtcaa tgcggccggc atgggcgaag ctacctgggg tgcaagcaag ggattggact
     2341 catcgttgta tctaaccatt gggacaggga taggcggtgg ttacatcaaa gatggcaaac
     2401 cactcatcgg tttgctgcat cccgaaatgg gacatatccg catcccgcac gaccgcaacc
     2461 tggacccgtt taatggcacc tgcccctttc acgatgactg cctggaggga ctcgcaagcg
     2521 ggcccgcaat agaaaaacga cttggtctca ctggggcctt catccctgag gacgatccgt
     2581 actgggagct tgaagcagaa tacattgcct cggcattgat gaatttcatc ctgatcttgt
     2641 cacccaaaaa gatcattctc ggtggaggcg tgatgcaacg tgaatatcta ttccccaaga
     2701 tccggcgcag ggtcgctgag ttgttgaatg gttatgtttc aagcaaaagc attttggaga
     2761 atatcgatag ctatattgtc ccaccggcat tgggaaatca atcgggcagt ttgggagcaa
     2821 tcgctttagc tatgtatcat ggtgaaaaga atcaatcttg aagttggaac tctgaaatgt
     2881 taatcataaa aggagtggat gatgtcaact aatgaagttc ctttgattcc ccgcaaactt
     2941 ttatttggca atccggataa gactcaggcg cgcatcagcc cggatggtca acgattgagt
     3001 tatctggcgc cggtcaacgg cgtgttgaac gtgtgggtcg ggccggtcaa cgatccggag
     3061 tccgccaagc cagtgacgaa tgacacca
//
LOCUS       DASTJP010000815        14792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1555678, whole genome shotgun sequence.
ACCESSION   DASTJP010000815 DASTJP010000000
VERSION     DASTJP010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14792
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555678"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFQ23_23795"
     CDS             <1..685
                     /locus_tag="VFQ23_23795"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HET9909691.1"
                     /translation="LKPYIRELFSLVQDGLKAEKHFLLEQAMGTFLDTDWGTYPFVTA
                     STTIPSAASAGLGIPPRYLTNIVGVTKAYTTRVGAGPLPTEIHDVESEAYKKFAEVAA
                     TTGRTRRVGWLDLELVKTAVELSGVTEICLTKLDVLSGLDEIQVCTKYELSCDPVRYA
                     DLDSYSLEKVETVYKTVPGWKEDISQARSFDELPENAQNYVKMIEDASGIPVKWIGVG
                     PERDATIRR"
     gene            complement(723..1352)
                     /locus_tag="VFQ23_23800"
     CDS             complement(723..1352)
                     /locus_tag="VFQ23_23800"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9909692.1"
                     /translation="MKLIIFGASGGTGLDVVKQALEAGHFVTAFVRTPSKISIQHPNL
                     TLFAGDVMNAGAVEKAIAAQEAVISVLGPVRPPVPHMMETAAKNIVAAMKKYGVRRIV
                     STTGAGVRQPEDQPKFMDRLIGVLLNLLAKEVVLDSAENVKVVQSSNLDWTIVRFPRL
                     MNGAHTGKYRVGYVSKDSSTQFSRADGADFILKELVERKWVRELPLVSY"
     gene            complement(1382..1681)
                     /locus_tag="VFQ23_23805"
     CDS             complement(1382..1681)
                     /locus_tag="VFQ23_23805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909693.1"
                     /translation="MLERLNEHVAFFLLIMSIILVTPLLSERVRLPGIIVIILGEMLI
                     GPLKKNCSRINLNPSFWKRKVLAVLWLCLGLDLVSALRIHWAIYPNASLHHLMGI"
     gene            1960..2214
                     /locus_tag="VFQ23_23810"
     CDS             1960..2214
                     /locus_tag="VFQ23_23810"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9909694.1"
                     /translation="MNFIVWIIFGALAGWIASMIMGKNAQMGALANIVVGILGALIGG
                     FLMTNVFGAQGVTGFNLTSLLVAIGGAVVLLFLVGMVRRS"
     gene            complement(2311..3135)
                     /locus_tag="VFQ23_23815"
     CDS             complement(2311..3135)
                     /locus_tag="VFQ23_23815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9909695.1"
                     /translation="MYRYPSAGRRRGSSLSGRLIFAAIIAIMSLVSYFGSRQDNPVTG
                     ETQYIGITVDQEIALGLQAAPQMAEEFGGLDPDESAQAIVDQIGNLIVQSSAASQTPY
                     QYEFHLLEDDQTINAFALPGGQIFITRALFDRLQTEGELAGVLGHEVGHVVARHSAEH
                     MAKAQLTEGLTGAAVIATYDPDNPASANSAQVAMLIGQMINMKFGRDDELESDFLGVC
                     FINDAGYNPEDLIRVMQVLAEASQGNAPPEFFSTHPNPDRRIERIQAAIQNISSCP"
     gene            3303..3554
                     /locus_tag="VFQ23_23820"
     CDS             3303..3554
                     /locus_tag="VFQ23_23820"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9909696.1"
                     /translation="MNIIVWIIFGALAGWIASMIMGANARMGALANIVVGIVGAIIGG
                     FLMTNVFGAQGVTGFNLPSLLVAIGGAVVLLFLAGLVRR"
     gene            3576..4175
                     /locus_tag="VFQ23_23825"
     CDS             3576..4175
                     /locus_tag="VFQ23_23825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909697.1"
                     /translation="MTIDISESKVAQNEMGMWLEWTLATAFGMLLGFLPSIIFVNSLD
                     LALARIIVPLFAGFLVGLAQWFVLRKYVNSVSDWIWSAGASWAVGYALGLLIINGLTG
                     TGLDGYIGYILFGAIVGLVQWPVLRREIPNVWIWILANVIGWTAGFFLSQVALDLFFN
                     GPAIAPVASTAVISGVSGLVAGAITGVALVMIVRQPERV"
     gene            4268..5350
                     /locus_tag="VFQ23_23830"
     CDS             4268..5350
                     /locus_tag="VFQ23_23830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9909698.1"
                     /translation="MKALSNLLDLFKRAYQDWKEDRASRLAASLAYYTIFSIAPLLVI
                     AIAVAGFIWERESVEAQVIGQIHGLVGTEGAIFVGSLIESASGQAEGVIATVVGIITL
                     LFGALGVFNELHNALNVIWEVKVEEAESFLDAVKKAIFDRLLSFTMVLGIGFLLLVSL
                     VISAGLSATQELLGNTFPIPEFFLQLINLIISIAVISVLFAMIYKYLPDAEIPWKYIW
                     PGAFATAILFSLGKLLIGLYLGNSAVASSFGAAGSLVLLLVWVYYSAQILFFGAEFTQ
                     VYANTYGPKILPDEKVTGAKTDSRPRKDTRDRVPKPLPNSSFEVRKDPVTNEKENQQF
                     ARFVLGLMVTSFLTGIMTTIWGLKKR"
     gene            5410..6348
                     /locus_tag="VFQ23_23835"
     CDS             5410..6348
                     /locus_tag="VFQ23_23835"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET9909699.1"
                     /translation="MPSLLILTDDTKKYLDLIQQAQLPNLEIFTEFHSECDIVFGDTG
                     RIKEALPKLSKPRWIQTTSAGIERLIDPSLRRDYVLTNARDVFGESMSEFVLGYILSF
                     KKRIFERYKSQQLGEWDNKDGGRLRGKTIGLLGVGSIGSHVAMTAKHFGMNVWGYTRS
                     SETSPYIDRYFHPQSESPSDKQAILAFARGSDYLVSVLPRTEGTDKIIDADLLNALPS
                     HAIVINVGRGNAMDEPALVEALNQKKLAGAVLDVTEQEPLPKDHAFWTTPNLLLTFHT
                     SAISYPEDMARLFIENYQLYVEGKPLRHQVDFEKGY"
     gene            6449..7402
                     /locus_tag="VFQ23_23840"
     CDS             6449..7402
                     /locus_tag="VFQ23_23840"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9909700.1"
                     /translation="MTHTLDDVRSAAERIKPYIHRTPVLTNQSLNQRTSAQVYLKCEN
                     LQKVGAFKFRGACNAVFSLGEEEARHGVSTHSSGNHAQALALAAQFRGIPAYIVMPSN
                     APRVKKEAVAGYGGLITFCEPTLEAREATLAKVAQETGSTIIHPYDNEQVIAGQGTVA
                     LEFLEDLPDLDVILAPVGGGGLISGTAIAATGLKAGIRVIAAEPEMADDAFRSLQIGS
                     IIPSKNPQTIADGLLTSLGTITFPIIQQHVEQIVTVSEAAIIESMKFIWERAKIIIEP
                     SAAVAIGVLWEKKIDLRGLNVGVILSGGNVDLERLPWQIRF"
     gene            7473..7847
                     /locus_tag="VFQ23_23845"
     CDS             7473..7847
                     /locus_tag="VFQ23_23845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909701.1"
                     /translation="MDEHSPIEERLPYKTRPLFFPNGASVEEFIIPDEDREIVLEQLY
                     PFSPIPKLTDVLYDLHADKTFVVKDFRVTWEHGMNYLVSPYYSEGGGTVIDWVDEDWA
                     NEDGEDDDFDEDMDDKEGGSLF"
     gene            complement(7912..8766)
                     /locus_tag="VFQ23_23850"
     CDS             complement(7912..8766)
                     /locus_tag="VFQ23_23850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET9909702.1"
                     /translation="MLKLIRPLHLFLAALTYTLGISLANYLGHPFSANIFWLGLVVSL
                     LTQLSMSLLADVFRLDVEPLLEKETRSERQTLRNNTLYVSIASLAVIAFITYLLFVNH
                     QLPAAAFFFLVLSLILILVYSLPQFRGRGLSEFLLAVQLAYVFPSFAFILQSGETHRF
                     LTLTIPLVFLAFAYFILMTFQSFASDRKYGRVTFLTQLGWERVVPLHHFLVLLAYLLF
                     FTSPAFGVSLSLIWRVFLALPFAIFQILQLRSISLGAPPNWALLSVTAFATFGLTTYL
                     LMLTFWLR"
     gene            complement(8840..9961)
                     /locus_tag="VFQ23_23855"
     CDS             complement(8840..9961)
                     /locus_tag="VFQ23_23855"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9909703.1"
                     /translation="MNLPPFKLERYFAKYEFITEFLLCSSDCEAMSIGDLLAVEPDAA
                     KKFQDVWLGYTESQGSPTLRKEISNLYQTISPEDVLVHAGAEEAIYLFMFAAFKKGDH
                     VIVHSPGYQSLAEVARAAGCDVSPWRAREENNWALDLDELRHLMRSNTKALVINTPHN
                     PTGFLMPRADYEDIHRFAQEKKLLLFSDEVYRESEYDVADRLPAACDFGEHAVSLGVT
                     SKTYGLAGLRIGWIATKNKKIYEAMASLKDYTTICNSAPSEFLAELAMRNRQTLAQRN
                     LDIIKHNLTVIDDLFARREDLFSWIRPKAGSMAFPRLLRGDVEDFCEDLVKTAGVLLL
                     PGSMYDHSRNHFRLGLGRKNLPQAVERLEAYLSKHKITG"
     gene            complement(10069..10539)
                     /locus_tag="VFQ23_23860"
     CDS             complement(10069..10539)
                     /locus_tag="VFQ23_23860"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9909704.1"
                     /translation="MSETSELAEKLKSEGEKVVTFFSEFRAGDWEREVYTEDTTWTVR
                     NILSHLVTSERGLIKLFERIRHGGEGAALDFSIDRYNASMQAKNKDLTEAELLNQYQE
                     VRANSIIWVSNLKEDELEITGRHPFLGVTTIREMIKMLYIHNQTHSRDMKKALK"
     gene            complement(10579..11283)
                     /locus_tag="VFQ23_23865"
     CDS             complement(10579..11283)
                     /locus_tag="VFQ23_23865"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9909705.1"
                     /translation="MPSFALVTGAAHRLGKTFALTLARLGYNIVLHYHSAEDEALQTQ
                     AEIESLGRRVTLVEADLTDPTQIQSLTSSLESLHVLVNSAAYMPGGNVNELSLENWDI
                     SLDLNLRAPFLLAQSVSKKMADNGLIVNITDTGAQKAWSRFPSYTVSKAGLESLTRIL
                     ARALAPKIRVNAIAPGFVLPSDIVPTEEWERLIKRVPLQRPARPDEIASALEFLLKNE
                     YVTGQVIVVDGGYSLI"
     gene            complement(11332..12297)
                     /locus_tag="VFQ23_23870"
     CDS             complement(11332..12297)
                     /locus_tag="VFQ23_23870"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET9909706.1"
                     /translation="MLRSFLISLSRAAWAQKLITSWSFAWRAASRFVAGERTEDVIRV
                     IRELNKKGFNATLNYLGESISTAEEVERATDEILKVLNEVDKADVKSNVSIKLTQIGI
                     GLDENICRQSLVQILEQVKKCGNFLRVDMEDTPYTDITISLYHSMLERGFDQTQLGLV
                     LQAYLFRTEADLRKLLEIGARIRLVKGAYNEPPDKAYSKKADTDANYDLLTKITIDAA
                     LKMDRNEISADGKIPPMIAIASHDEKRIGFAKQYAEKVGLPKNAIEFQMLYGIRRDLQ
                     QQLLKDGYPVRVYVPYGSQWYPYFMRRLAERPENIWFFISNYFRN"
     gene            12586..14091
                     /locus_tag="VFQ23_23875"
     CDS             12586..14091
                     /locus_tag="VFQ23_23875"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9909707.1"
                     /translation="MRNRRSIPILRGASIAVLSIALVLVVISLIGYSRQRNNYPSGMT
                     IAGVQVGGVNPQIATQRVLQVYSSPIEVQYGEAVIHIEPTVVGFELELDTMIAAADLA
                     RTGGSFWGGFWNYLWNRDPQLVEVPLTASMAEERLVSYLQNEIAARYDEPPTPAQPIA
                     GTTNFSPGSPGRVLDIESAVRLIEDTLRSPTNRTVVLSFQRSAAARPTMQNLEILLQQ
                     NISVAEYDGVIGVYLLDLQTGQEIHFAMNQGEKIEVNPDVAFTASSTIKIPIMVSYFI
                     KYGPPAINTDAMQLIQSAMQTSENALADVLLNELDPNRGPLIVTENMRMIGLENTFLA
                     GFFCNPDFPCPLLERISTPANQRTDVSTDPDAFNQTTPSDAGMLLADIYQCATTGGGA
                     LIAAFPDKINPQICQQMISFLAADKIGVLIEAGVPEGTQVAHKHGWITEPSSGVIQNI
                     SDAGIVYTPGGNYVLVIYAYHPVQTIWEPVSALFAQMSQAVYNFYNIPTTQ"
     gene            14115..>14792
                     /locus_tag="VFQ23_23880"
     CDS             14115..>14792
                     /locus_tag="VFQ23_23880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909708.1"
                     /translation="MSAALGLYRLQQVDSQIDQIQARLKLIRETLENDRELQTATRNF
                     TSADNVYKDAQRALKQSEGEVEKQRIKIEQAEASLYGGRVHNPKELQDLQKDVASLKR
                     YLETLEERELEAMLVVETSENESKAAKANLDHVQSNRSDQNRDLMQESDLLHKNLERL
                     NTERQAVIQNLAQQKMGVYDQLRLQRRGIAVTTISDAACAACGTTLTPSQQQNARSTS
                     QLFHCPSC"
BASE COUNT         3920 a         3521 c         3563 g         3788 t
ORIGIN      
        1 cctgaagcca tatattcgcg agttgttctc gctggtgcag gatggcttga aagcagaaaa
       61 acatttcttg ctcgaacaag ccatgggaac ttttctagat acagattggg gcacctaccc
      121 atttgtgact gcttcaacga cgattccctc ggcagcatca gccggtcttg gaatcccgcc
      181 gcgctatctc acgaacattg ttggtgtgac aaaggcttac accacgcgag tgggcgccgg
      241 accgttgcca acagaaatcc acgatgtgga aagcgaagct tataagaagt tcgctgaagt
      301 cgctgcaacc actgggcgca cacggcgagt cggctggctg gatctggagc tcgtgaaaac
      361 tgctgtcgag ctaagcggcg tcactgagat atgcttgacc aaactagacg tattgagtgg
      421 cttggatgaa attcaagtgt gcacaaaata tgaattgagt tgcgaccctg taagatatgc
      481 agatttggac tcttacagcc ttgaaaaggt ggaaacggta tacaaaaccg ttcccggctg
      541 gaaagaagat atcagccagg cgaggtcatt tgatgaattg ccagagaacg cgcaaaatta
      601 tgtgaagatg atcgaagatg cgtctggcat tcctgtgaaa tggattgggg tgggtcctga
      661 gagagacgcg accattagga gataatactt caaacgccga agggtcttaa gaattttcca
      721 ccttaataac tcaccagcgg caactccctc acccactttc tctctaccaa ttccttcaaa
      781 ataaaatccg cgccatcggc gcgggaaaat tgtgtgctgg aatctttact tacataacca
      841 acacgatatt tgccggtgtg tgcgccattc atcaatcggg gaaaacgcac aatcgtccaa
      901 tccaggttgg aagattgaac tactttcacg ttctcagctg agtccagcac gacttctttt
      961 gctaagagat taagcaatac gccaatgaga cgatccatga atttcggctg gtcttctggc
     1021 tgacgtaccc ccgcaccggt tgtcgaaacg attcggcgaa cgccatattt cttcatcgcg
     1081 gccacgatat ttttcgcggc cgtttccatc atgtgaggca ccggagggcg gacgggtccg
     1141 agaacgctga tcactgcctc ctgagcggca atcgcttttt ccactgctcc agcattcatc
     1201 acatcgcctg cgaacagagt caggttcgga tgttgaatgc taattttcga tggcgtacgc
     1261 acaaaggcag tcacgaaatg cccggcttcg agcgcttgtt ttacgacatc caaaccggtg
     1321 ccgcctgagg caccaaagat gatgagtttc atcctcgttt acttatcaaa atgcaataca
     1381 gctagattcc catcaaatga tgcagcgagg cgttcgggta gattgcccaa tgtatccgca
     1441 acgcgcttac gagatccaaa cccaggcaca accataaaac tgctaatact ttcctcttcc
     1501 agaatgacgg gtttaagttg atccttgagc aattcttttt caatgggccg atcagcattt
     1561 ccccaagaat aataacgatg atacccggca agcgcacccg ctccgatagc agcggtgtca
     1621 ccaggataat actcatgatt aaaagaaaaa aagcgacatg ttcatttaat cgttcaagca
     1681 cgaaatacct cctaagaata ttttttcgat tttacacgac tgacaatctg ataattccaa
     1741 aaaaatccat taaatttgtc agaagatgct gtttaccgta actccatacg aatcggaggg
     1801 ttgaaatata taaagccgag cgtgcaatac gtggccgtgg ttacgtcact ctgatgtgag
     1861 taacatttga aaattgcctt tgtatcttat tcataaacag atgcgatctg ccagagtaag
     1921 gacaggcaat ccagtattct tatttctatg gaggtagaaa tgaatttcat tgtatggatc
     1981 atctttggtg ctcttgctgg atggatcgca agcatgatca tgggtaagaa tgctcagatg
     2041 ggtgcactgg cgaacatcgt ggtaggcatt ctgggcgctc tgattggtgg ttttctgatg
     2101 accaatgtct tcggcgcgca gggagtcacc ggcttcaacc tcaccagcct gctcgtcgcc
     2161 attggaggtg cggtggtttt gctcttcctg gtaggcatgg ttcggcgttc ctaaaattca
     2221 gatttagtca aaaacaccaa aagaagccac gcacgaatgc gtggcttctt cgttatttat
     2281 tgtttcgtcg agtttcccaa tgtggttcaa ctacgggcat gagctaatat tttgtatggc
     2341 agcctgaatg cgctcaatgc gtcgatcggg gttcggatgt gtactaaaga attccggcgg
     2401 cgcatttccc tggcttgcct ctgccagaac ctgcatcact cggatcagat cttcagggtt
     2461 gtatccagca tcatttatga aacagacgcc gagaaaatcc gactcgagtt catcatctct
     2521 tccgaacttc atattaatca tttgcccaat caacattgca acctgtgcag aattcgcgct
     2581 ggcagggtta tccggatcat aggtggcaat gactgcggca cccgtcagcc cttctgtgag
     2641 ttgtgccttt gccatgtgct ctgccgaatg gcgcgcaacc acgtgcccaa cttcatggcc
     2701 cagcacaccc gccagttctc cttcagtttg aagtcggtca aacaacgcgc gtgtgataaa
     2761 tatctggccg ccgggcaaag caaatgcatt gatggtttga tcatcttcca ggagatgaaa
     2821 ctcgtattga taaggcgtct ggctggcagc agagctttgc acaatcaaat ttccaatttg
     2881 atccacgatc gcctgtgcac tctcgtcagg gtcaaggcca ccaaactcct ctgccatttg
     2941 aggtgctgct tgtaaaccca gagcaatttc ctgatcgacc gtgataccga tatactgtgt
     3001 ttcacctgtt acagggttgt cctgcctgga tccaaaataa ctaacaagcg acatgatcgc
     3061 gatgattgca gcgaagatca gacgaccact caatgacgag ccccgccgtc tccctgcact
     3121 tgggtaccga tacatatcat tctccttcgt ttattaatga acggtaatca attgattgaa
     3181 aacaaaaata tactcttgta ctctaaatct tcctacgggg gaaatcccta attgcttatc
     3241 tatccgttcc acatatactt taagccagca ttaccaaaca aacactttac tcggaggatg
     3301 aaatgaacat tattgtatgg attatctttg gtgcacttgc aggctggatt gccagcatga
     3361 tcatgggcgc caatgcccga atgggcgccc tggctaatat tgttgtcggt attgtgggtg
     3421 ccattatcgg tggatttctc atgaccaatg tctttggtgc gcaaggcgtc acaggcttca
     3481 acctgcccag cttactagtc gccatcggcg gcgcagtcgt tttgctcttc ctggctggat
     3541 tggttcgacg ctaagcaatc tgcaaatgct actatatgac tatcgatatt agtgaatcaa
     3601 aagtagcaca aaatgaaatg ggcatgtggc tagagtggac tctagccact gcctttggaa
     3661 tgctattggg atttttaccc tcgatcatat ttgtcaacag cctggatctg gcacttgctc
     3721 ggatcattgt gccactcttt gcaggttttc ttgttggtct ggcacaatgg tttgtattgc
     3781 gcaaatacgt aaatagcgtc tcagattgga tatggtctgc aggagcaagc tgggcagtcg
     3841 gatatgccct tggtttgctc atcataaacg gactgaccgg gacagggctg gacggataca
     3901 ttggttatat cctttttggt gcaatcgtcg gactcgtcca gtggccggtt ttgcgaagag
     3961 aaattcccaa tgtgtggatt tggattctag ccaacgtgat cgggtggact gcaggtttct
     4021 ttttgagtca agtcgcgctg gatctttttt tcaatggtcc cgcaatcgct ccagtggcca
     4081 gcactgcggt gatctcgggt gtaagcggtc tggtggctgg cgccattaca ggcgtagcac
     4141 tggttatgat cgttcgtcaa cctgaacgcg tttagacttc gaccactggt ttttcaaagg
     4201 atctcacttc ccaggcccgt tgcgaggaag atgagattct ttttatttcc tcataaggat
     4261 agatgaaatg aaagcgcttt ctaatttatt ggatctcttc aaacgagctt atcaggattg
     4321 gaaggaagat cgagcgtccc gattggcggc ctcactcgct tattacacaa ttttttcgat
     4381 cgcgccgttg ttggtaatcg ccatagcagt ggctgggttt atctgggaac gagaatctgt
     4441 tgaagctcag gttatcggac agattcacgg ccttgtcgga acggaagggg ccatttttgt
     4501 tggcagcttg atcgagagcg ccagcggtca ggctgaaggg gtaattgcga ctgttgtcgg
     4561 aatcatcacg ttgttattcg gtgcgctggg tgttttcaac gaattgcata atgcccttaa
     4621 cgttatttgg gaggtgaagg tagaggaagc agaaagcttc cttgacgcag ttaaaaaggc
     4681 aatatttgac cgcctcctct ccttcacaat ggttttggga attggttttc tattactggt
     4741 ttcactcgta atcagcgccg gactctccgc tacgcaggaa ttacttggaa ataccttccc
     4801 catccctgaa tttttcctgc aattgatcaa tttgattatt tctattgctg tgatttctgt
     4861 actctttgcc atgatttata aatacttgcc cgacgcagaa ataccctgga aatacatttg
     4921 gcctggtgct tttgccactg cgattctgtt ttcgttgggt aaactattga ttgggctcta
     4981 cttaggaaat agcgcggtcg catcttcctt tggcgcggca ggatcattag tcttgctttt
     5041 ggtttgggtc tattattccg ctcaaatcct tttctttggt gcggaattta ctcaggtata
     5101 cgcaaataca tatggtccaa aaattctgcc tgatgaaaaa gtaacaggcg cgaaaactga
     5161 ttccagacca agaaaagaca ccagagatcg agttcccaaa ccgctaccca attcatcttt
     5221 tgaagtaaga aaagatcccg ttacgaatga aaaggaaaat cagcagtttg cgcgtttcgt
     5281 tttaggactg atggtcacat cctttcttac cggaatcatg acaacaatct ggggcttgaa
     5341 aaagaggtaa accgaaaact gagaaataag taggcacgaa gggttgccta gttcgaatat
     5401 aatctcacta tgcccagcct cctcatcctt acagacgaca caaagaaata tctggacctg
     5461 atacagcaag ctcaattgcc gaaccttgaa atcttcacgg aatttcattc ggagtgtgat
     5521 atcgttttcg gcgatacagg acggataaaa gaagcactcc ccaaactctc aaaacccagg
     5581 tggatacaga cgacatcagc cggtatcgag cgtctcatag acccttccct gcgacgcgac
     5641 tatgtgctga ccaatgcaag agatgttttt ggtgagtcca tgtctgagtt tgtgctcggg
     5701 tacattctct cgttcaaaaa aagaatcttt gagcgctaca agtctcaaca attgggagaa
     5761 tgggataata aggatggcgg ccgactgcgc ggaaaaacga tcggcctgct cggtgtcggt
     5821 tcgatcggat cgcatgtagc catgacggcc aagcatttcg gtatgaacgt ctgggggtat
     5881 acaagatcca gtgaaacaag cccatacatt gataggtact ttcatcctca atctgaatcg
     5941 ccctctgaca aacaggcgat tctggcattc gccagggggt cggattattt agtttcagtt
     6001 ctacctcgca cagaaggcac ggacaaaatc atcgatgcag acctgctcaa cgcgttgcca
     6061 tctcatgcga ttgtgatcaa tgtggggcgt ggaaatgcaa tggacgagcc agccctggtg
     6121 gaagcactta atcaaaaaaa gcttgcgggc gctgttttgg atgtcacgga gcaagagcca
     6181 ctcccaaaag atcatgcttt ttggactaca ccaaatttat tactgacctt tcatacatcc
     6241 gcgatcagtt atccggaaga catggcacgg ctatttattg agaattacca actttatgtt
     6301 gaaggcaaac ctctacgaca ccaggtcgat tttgaaaaag gctactagcc tttgcgtcgc
     6361 ggagtcaaag ggatacatct cttcctattg actcgggatt tacatttctt ggcaaactgg
     6421 acccaggtct gacggccggt ggagaagcat gacccatact ctcgatgacg ttcgaagcgc
     6481 agcagagcga attaagcctt acatacatcg cacgcctgta ctgacaaacc aaagcttaaa
     6541 tcagcgtact agtgcgcagg tgtatctcaa atgtgaaaac ctgcaaaaag tcggtgcttt
     6601 caagtttcgt ggcgcctgca acgccgtgtt ttcattgggt gaagaggaag ccaggcacgg
     6661 tgtatccacg cattcctctg ggaatcatgc gcaggcgttg gcccttgcgg cacaatttcg
     6721 cggtatcccg gcttatattg tcatgccgag taatgcgcct cgggttaaga aagaggcagt
     6781 ggcaggctat ggaggactaa tcacgttctg tgagccaact ttggaagcac gcgaggcgac
     6841 gcttgcgaag gtcgcgcaag aaacgggctc aaccatcatt catccctatg acaacgagca
     6901 agtgatcgcg ggtcaaggta ccgtagcgct ggagttctta gaagatctgc ctgacctgga
     6961 tgtgatcctc gcgccagttg gcggtggtgg cttgatcagt ggaacagcca tcgcggcgac
     7021 tgggctcaaa gccggaatcc gtgtcattgc ggcagaaccg gaaatggcag acgatgcatt
     7081 tcgttcctta caaattggca gcattatccc ctctaagaat ccacaaacga ttgcagatgg
     7141 tttgttgact tcactcggaa caattacatt tccaataatt caacaacatg tcgagcagat
     7201 tgtcacagta agcgaagcgg cgattatcga atcgatgaag ttcatttggg aacgcgcaaa
     7261 gatcattatc gagccttccg ctgctgttgc aatcggtgtg ctatgggaaa agaaaattga
     7321 tttgcgagga ttgaatgtgg gcgtcatcct gagcggcgga aatgtggacc tggaaaggct
     7381 gccctggcaa attcgatttt gacaaaaatt tatgcaggcg cgttacaatt atttcagaca
     7441 agttgcagtg tggcatttga caaggagata aaatggacga acactcaccg attgaagaga
     7501 gattgccgta taaaacacgt ccacttttct ttccgaacgg tgccagcgtg gaagagttca
     7561 tcatcccgga tgaagacagg gaaattgtcc ttgaacagct ctatcctttt tcacccatcc
     7621 caaaattaac ggatgttctc tatgacctgc atgccgacaa aacttttgtg gtgaaagatt
     7681 tccgcgtcac ctgggagcac ggcatgaatt atcttgtcag cccctactac tctgaaggcg
     7741 gtgggactgt cattgactgg gtggatgaag actgggcaaa tgaggacggt gaggacgatg
     7801 attttgatga ggatatggat gataaagagg gtgggtcgct gttttagatc agtgtcacgt
     7861 ccccagtagt tggtaaaaga ataaaggtaa agaaaacggc caacaccagg ctcagcgaag
     7921 ccaaaacgtc agcatgagaa gatatgtcgt cagcccgaag gttgcaaagg cggttacgga
     7981 gaggagggcc cagttgggcg gagctccgag agaaatgctt ctcaactgaa ggatttgaaa
     8041 aatcgcgaat ggcagagcga gaaacacacg ccaaatcagc gacagtgaaa cgccaaaagc
     8101 cggggatgtg aaaaagagta gataagccag caaaacaaga aaatgatgta acggaaccac
     8161 ccgttcccat ccaagctggg tgaggaaagt gacgcgtcca tacttgcggt cagaggcaaa
     8221 ggattggaag gtcatcagga tgaagtatgc aaacgctaag aacacaagcg ggatggtgag
     8281 ggtgagaaaa cgatgcgtct cgcctgattg aaggatgaac gcaaacgatg ggaagacgta
     8341 cgccaattgc acagccagca ggaattcgct gagacctcgg ccgcgaaatt gtggaagaga
     8401 atagacaaga atgaggatca gggataggac aagaaaaaag aatgccgctg caggtaactg
     8461 gtgattgaca aataatagat aagtaatgaa tgcaatcaca gccagagatg caattgaaac
     8521 gtacaaagtg ttgttgcgca atgtttgacg ctctgagcgc gtttcctttt cgaggagtgg
     8581 ctccacatca agacgaaata catctgccaa cagactcatg ctcagttggg ttaacagact
     8641 gacaacaagc cccaaccaga atatatttgc gctaaaagga tgcccaagat aatttgcaag
     8701 gctaatacca agggtatagg tcagcgctgc cagaaagagg tgaaggggac gaatgagttt
     8761 taacaattta tgaattccga ttttcgatgg agggttttgt aacgagtcgg cttaatagtc
     8821 actgttagtc tatcaacatt caaccagtga tcttatgctt tgaaagatac gcttccagtc
     8881 tttcaactgc ctgcggaaga ttcttccgtc ccagacccag acgaaagtgg ttccttgaat
     8941 gatcatacat cgaacccggc aacagcaata cacctgcggt tttgaccagg tcctcacaaa
     9001 agtcttcaac atcacctctc aataaacgag ggaaagccat tgagccggct ttcggtcgaa
     9061 tccacgaaaa taaatcctcg cgacgagcaa aaagatcatc gatcacagta aggttatgct
     9121 tgatgatatc aagatttcgc tgtgccaacg tttgtcgatt ccgcatcgcg agttcagcca
     9181 gaaactcact tggcgcagaa ttacagatcg tcgtgtaatc cttcagagat gccatcgcct
     9241 catagatttt cttgttcttt gtagcaatcc atccgatacg cagcccggca agtccatatg
     9301 ttttggatgt gactcccagg gagactgcat gctcgccgaa gtcgcaagcg gccgggagcc
     9361 gatccgcaac gtcatattca gactctcgat agacctcatc agaaaatagc agaagctttt
     9421 tctcctgcgc aaatcgatga atatcttcat aatccgcgcg aggcatcaaa aagcccgtcg
     9481 ggttgtgcgg cgtgttgata acaagtgctt tcgtattgct tcgcatgaga tgacgaagct
     9541 catccaaatc gagagcccag ttattctcct cacgtgcgcg ccaggggctc acatcgcaac
     9601 ccgccgcgcg cgccacctcc gccagagatt gatatcccgg cgagtgcacg ataacgtgat
     9661 cgcctttttt gaacgccgcg aacatgaaga gataaatagc ttcttctgca cccgcatgaa
     9721 cgagaacatc ttcgggtgaa atcgtttgat aaagatttga aatctctttc cgcagtgttg
     9781 ggctgccctg tgattcggtg taacccagcc atacatcctg gaatttcttt gcggcatcag
     9841 gttccactgc aagcaaatct ccaatagaca ttgcttcaca atctgacgaa caaagaagaa
     9901 attcggtaat aaattcgtat ttggcgaagt agcgctcgag tttgaagggt ggaagattca
     9961 tgaaaacctt tcggtcattg cgagcgagca ttcttatgcg agcgaagcaa tctccaactt
    10021 aaccagggga ttgcttcgtc gggcttacgt cctcctcgca atgacgtatt atttcaatgc
    10081 ttttttcata tctcgtgaat gagtttgatt atggatatat aacattttta tcatttctcg
    10141 aatagttgtc acgccgagaa acggatgtcg ccctgtgatt tcaagttcat cctccttcag
    10201 attggaaacc catataatgg aatttgcgcg cacctcctga tattgattga gcaactcagc
    10261 ctccgtcaaa tccttgttct ttgcctgcat actggcattg tagcggtcaa tcgaaaaatc
    10321 cagggctgcc ccctccccac catgacggat tctctcaaat agtttgatga gccctcgctc
    10381 tgaggttacc aggtgcgaca agatattgcg aacggtccag gtagtgtctt ctgtgtagac
    10441 ttccctttcc caatcgcccg ctcgaaattc actgaaaaat gtgacaacct tttcaccttc
    10501 tgattttaat ttttcggcca actcagaggt ttcagacatt ttagcttctc ctgaaagcgc
    10561 gttttgacat cttttacatt aaatcaatga atacccgccg tcaacaacaa tgacctgacc
    10621 agtaacatac tcattcttca ataaaaattc aagggcggat gcaatctcat caggtcgtgc
    10681 gggacgttgg agcggcaccc tcttgatcag cctttcccac tcttcggttg gaacgatatc
    10741 tgatggcaaa acaaagccgg gcgcaattgc gttcacccgg atcttcggcg ccaaggcgcg
    10801 tgccagaatc ctggttagcg attcaagtcc ggctttactg actgtataag acggaaaacg
    10861 actccacgcc ttctgggcgc ccgtatcggt aatatttacg atgagaccat tatctgccat
    10921 ttttttagat accgattgag caaggagaaa gggggctctg agatttagat caagagagat
    10981 atcccagttt tcgagggaaa gctcatttac atttccgcct ggcatatacg cagcagagtt
    11041 cactaaaaca tgaagagatt ctaaactcga tgttagagat tggatttgtg tgggatcagt
    11101 caggtcagct tcaactaagg tcacgcgcct tccaagagat tcgatttcag cctgagtctg
    11161 aagtgcctca tcttcggcag aatgatagtg aagcacgata ttgtagccga gacgggcaag
    11221 agtcagggca aaagtttttc ccagccggtg tgccgcgcca gtgaccagag caaaagaggg
    11281 catgcagcaa ttataaaccg ccctacccct ccctcccgcg gaacgatgac gttagtttcg
    11341 gaaataattt gaaataaaga accagatatt ctcaggtcgt tctgccagac gtcgcataaa
    11401 ataaggatac cactgtgatc cataggggac atagacacgc acaggatatc catccttcaa
    11461 taattgctgc tgcaaatcgc gacggatgcc gtacaacatc tgaaactcga ttgcattttt
    11521 cgggagtcca actttttcag catattgctt tgcaaacccg atacgctttt cgtcatgcga
    11581 ggcaatggca atcatcggcg gaatcttccc gtccgcgctg atctcattcc tatccatctt
    11641 caaggccgcg tcaatcgtga tctttgtcag aaggtcataa tttgcatccg tatcggcctt
    11701 tttggagtac gctttatcgg gtggttcatt gtaagcaccc ttgacgaggc ggatacgggc
    11761 cccgatttcg agcagtttcc gcaaatctgc ctccgtgcga aaaagatagg cttgcaacac
    11821 cagccccagt tgagtctgat caaagcctct ttccagcatg gagtgataga gcgaaattgt
    11881 gatgtctgta tagggagtat cttccatatc cacccgtaga aagtttccac actttttcac
    11941 ctgttcaaga atctgaacca gactttgccg gcaaatattt tcatccaagc caatgccgat
    12001 ctgggtcagc ttaatagaga cattggattt aacgtccgcc ttatctactt cgtttaaaac
    12061 tttaaggatt tcatcggttg ctctttccac ctcttcagcg gtagatatgc tttctcccaa
    12121 ataattgagc gtggcattga agcccttctt gtttaattcg cgaatcacac gaattacgtc
    12181 ttctgttcgt tcccccgcta cgaatcggga cgctgcccgc caggcaaagc tccaactcgt
    12241 aatcagtttc tgtgcccatg cagcccttga aagagaaata aggaaagagc ggagcatgat
    12301 cccgtcctac agataaaatt tattcctgcg tgaacgaaaa cgcaggaggt ggagatattc
    12361 tagcacaaag cgtaccgctt gtctcgtcag caactaaaac ttttggactg atttgtagac
    12421 taaaagttaa atcttaaatt cacctaatta tgcggcactc tataaaaatg agtatgttat
    12481 actttttgcc gctttgcccc ataaattgct gaacctgcct gaactactaa ttggtcagat
    12541 ccaaattcta gtaatttatg cggcaccttg ccagccggag gaagtgtgag aaatcgacgt
    12601 tccattccca tcttgcgcgg tgcatccatc gcggtgttgt ccatcgcgct ggtgctggtc
    12661 gtcatctcgt tgatcggtta tagccgccag cgcaataact atccaagtgg aatgaccatt
    12721 gccggtgtgc aagtaggtgg ggtcaacccg caaattgcaa cccaaagggt cctgcaagtc
    12781 tattcgtcgc cgattgaggt gcaatatggt gaagcggtca ttcatatcga acccactgtg
    12841 gtcgggtttg agttggaact ggatacaatg atcgctgctg cagaccttgc tcgcacaggt
    12901 ggttcttttt ggggaggatt ttggaattac ctgtggaatc gagaccctca attagttgaa
    12961 gtccctctaa ctgcttcaat ggccgaagag cgcctggttt cctatcttca aaacgagatc
    13021 gcagcgcgct atgatgaacc tccaacgcca gctcaaccca ttgccggcac cacaaatttc
    13081 tctccgggtt cgccaggccg cgtcctggat atcgagagcg ccgtccgttt gatcgaagac
    13141 acactgcgtt ctcctacgaa tcggacggtg gtgctctctt tccaacgtag cgctgcagcc
    13201 cgaccaacga tgcagaatct ggaaattctt cttcagcaga atatctcggt tgctgaatat
    13261 gatggagtga tcggtgtata tttgctggac ctgcaaaccg gccaggaaat tcactttgcg
    13321 atgaatcagg gcgagaaaat tgaagtgaat cccgatgtgg ccttcacggc gtcgagcacc
    13381 atcaagattc ccattatggt ttcgtacttt atcaaatacg gacctcccgc catcaatacg
    13441 gatgcgatgc agttgattca aagcgctatg caaacgtctg aaaacgctct tgcagatgtg
    13501 ttgctgaacg aattggatcc aaatcgcggt ccgttgattg tgaccgagaa catgcggatg
    13561 atcgggttgg agaacacctt tcttgctgga ttcttctgca acccagactt tccatgtccg
    13621 cttttggaac gaatcagtac accggcaaat cagcggacag acgtctctac agacccggat
    13681 gcgttcaacc aaacgacacc ctcagatgca ggaatgcttc ttgctgacat ctatcaatgc
    13741 gctaccacgg gcggtggtgc tttgattgca gcattcccag acaaaatcaa tccgcaaatc
    13801 tgccaacaaa tgatcagctt tctggctgcc gataagatcg gcgttctgat tgaggccggc
    13861 gtcccagagg ggactcaggt tgcacacaaa catggatgga tcacagaacc gagctcaggc
    13921 gtcatccaga acatcagcga tgcggggatt gtttatacgc caggtggcaa ttacgtcttg
    13981 gtaatctacg cctatcatcc ggtccaaacg atttgggagc ccgtctcggc gctattcgcg
    14041 caaatgtctc aggcagttta caacttttat aacatcccca ccactcaata agggacttcg
    14101 tatataatcg aggtatgagt gctgcgcttg gactttaccg acttcaacag gtggatagcc
    14161 agatcgatca aattcaggcg cgtttgaagc tgatacgaga aacgctggaa aacgatcgcg
    14221 agcttcagac tgccacacgc aatttcacat ccgcagacaa tgtctataaa gatgcccagc
    14281 gcgctttgaa acaaagcgaa ggagaggttg agaagcaacg catcaaaatt gaacaggcgg
    14341 aagcaagtct ctatggcggg cgtgttcaca atcctaaaga attgcaggac ttacaaaaag
    14401 acgtcgcttc tctgaaacgt tatctcgaga cgttggagga acgtgaactg gaagctatgc
    14461 tcgtggttga aacatcagaa aacgaatcta aggctgcaaa agccaatctc gaccatgtac
    14521 aatccaatcg gagcgatcag aatcgtgatc tgatgcagga aagtgacctg cttcataaga
    14581 atcttgaacg gttgaatacc gaacgccagg ctgtgatcca gaatctcgcg caacagaaaa
    14641 tgggtgtata cgatcagctt cgtctacaac gacgtgggat cgccgtcaca actatcagcg
    14701 acgccgcctg cgctgcatgt ggtacgacac ttacgccttc acaacagcaa aatgcgcgtt
    14761 ccacgagcca attgttccac tgcccatcct gt
//
LOCUS       DASTJP010000816         3666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1239127, whole genome shotgun sequence.
ACCESSION   DASTJP010000816 DASTJP010000000
VERSION     DASTJP010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239127"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFQ23_23885"
     CDS             complement(<1..451)
                     /locus_tag="VFQ23_23885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909709.1"
                     /translation="MKHPLELTVMMYHYIRDRGDEAEAGSGIPGMTVHTFETQLDELS
                     KQHMFVDWPAVRAAVQEDKPLPSSACLLTFDDGVLDHYLNVFKILHRRNLSGLFFAMS
                     RCEDAGLTLPHKIHFLLAKLGLTSLRDAIWEKLSAEQKEQFAHAEKKY"
     gene            complement(448..2757)
                     /locus_tag="VFQ23_23890"
     CDS             complement(448..2757)
                     /locus_tag="VFQ23_23890"
                     /inference="COORDINATES: protein motif:HMM:NF021534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HET9909710.1"
                     /translation="MTENFPDFDFELSPYTGWTRAHWEYIFARMTYGYAKIAEQTGSP
                     ARVLYPDDRRGLPDSVDAIESFARIASAWGAWLRNPANPAVIKFHGHEMNLETLLGQA
                     LLDGTNSSNPYTYWGDITHLDQRIVESADIAVAIWMSRERVFNKMTKAEQEQIMTWLA
                     QVDNKGTYTDNWILFTAMAQAVRHYLGFPSPVDDLDYRLLQIGEFYRGDGWYVDGPTD
                     EFELYNAWMFGWHYFLWTYIDGDRRPEHRELVRQRGRSFIDGFLHFFGSNGSYPAWGR
                     SIVYRFAALAPFAVGHFLKIAPEDPGLLRRVSSGCIRYFHDHGLFDSDGHFVRQGYHG
                     EFPPAGEAYISPGSPYWCCHGLFALTLDRDDPFWTATEKPLPVEKDDYDLVLPAPGYV
                     VSGRKDTGQVLLLNSRSGQEHDAPRHNYTSKYGKLTFSTHFPFNVLPVRGSYAPDAMV
                     SLTRDGKSFGHRGHTRTGGVAPGFMWSKFDQLIDDELQPLWTSVLLWRDIQLRLTVIR
                     PTLGVTAYEAPGALGCEQAAHVIRSSDLSAGWEYAESEGRAVAIQRMFGYDRQKTSAP
                     FLDQSNINLAYTYSEQPMIYESEARMTARCLASASLVRPSSFDPASEFSGINVEVEYP
                     EIFHVTLPDGTLALAAPGETTPKRATINGVEVEGKQIRYMQVSKDLNEVCGLGLTHIA
                     GMVSFTEAATFRLKRISPVEILISTSAGFTLHDPLLGGNARCIETRSLDGQWFDVTSQ
                     CQNNSIPTQVVQEYSDCNQRTLVEFRVCL"
     gene            complement(2763..>3666)
                     /locus_tag="VFQ23_23895"
     CDS             complement(2763..>3666)
                     /locus_tag="VFQ23_23895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9909711.1"
                     /translation="LGFDSVWLEEHHSIRNHYWPSPLMALAGIATRTSKVLLGTDIVV
                     LPFYHPVRVAEDTAMLDVISDGRVIFGAAIGYRPPEFELYDVSLDDRGARYVEMLKIM
                     RALWTQDRVEYDGRFFKIKGSIEPRPENPIPIWLGGWGELSLKRAAQLGDAWVPGPTA
                     NLEKLLNAQKQYRAFLSDAGKNPADVPNPLTREVVIAETREEALELAEKYVMVNYRDE
                     YGGGWKHPLIGSQDQTAIALESLSQDRFIVGNPDDCIAHIKRFVDTFGVDHLICRLYF
                     AGMPHDHIMKELKLLSQEVFPAFR"
BASE COUNT          943 a          966 c          913 g          844 t
ORIGIN      
        1 gatatttctt ctcagcatgg gcgaattgtt ctttctgttc tgcagacaat ttctcccata
       61 tggcatcccg caagctagta agtcccaatt ttgccaacag gaaatgaatc ttatgaggca
      121 gggtcagccc tgcatcttca cagcgactca tggcaaaaaa taagccagaa agatttcgcc
      181 gatgaaggat cttgaacacg ttgagatagt gatcgagaac accatcatca aaggtcagta
      241 aacacgctga acttggcagc ggcttgtctt cctgcacggc cgctcgtaca gcgggccagt
      301 cgacaaacat gtgttgcttt gacaattcat ccaattgcgt ttcgaacgta tggacggtca
      361 tccccggaat gcccgaaccc gcctcggcct catcaccgcg gtcgcgtata tagtggtaca
      421 tcatgacagt caattcaaga ggatgcttca tagacatacc cggaattcca ccagagttcg
      481 ctggttgcaa tcagaatatt cctgcactac ctgtgtggga atggaattat tttgacattg
      541 tgaggttaca tcgaaccatt gaccatccag gctgcgagtt tcaatgcagc gagcgtttcc
      601 gcccagaagt ggatcgtgca gtgtaaaacc ggcactcgta gaaataagga tttcgaccgg
      661 cgatatacgt ttcagccgaa atgtcgcagc ttctgtaaat gagaccatgc ccgcgatatg
      721 ggttaatccc aaaccacaaa cttcattcaa gtcttttgac acctgcatgt agcggatttg
      781 ttttccttcc acttcgacac cgttgatcgt ggcgcgcttc ggagtagttt caccaggcgc
      841 agccagggct aaagttccat ccggtaaggt gacatgaaag atttccgggt actccacttc
      901 cacgttgatt ccagaaaact cactcgccgg atcaaacgac gaaggacgaa cgagtgatgc
      961 ggatgcaaga cagcgcgcgg tcattcgggc ctcggactcg taaatcatcg gttgttcgga
     1021 ataggtgtaa gccagattga tatttgactg atccaaaaac ggcgcgctgg tcttctgcct
     1081 gtcgtagccg aacatccgct gaatcgcgac cgcccgacct tctgattctg cgtattccca
     1141 acctgctgat aaatcagagc tgcgaatcac atgcgcggct tgttcgcaac ccaatgcacc
     1201 cggcgcttcg taagcagtaa ctccaagggt cgggcgtatg actgtcaatc tgagttgtat
     1261 atctcgccat aaaagcacag aagtccaaag tggttgcagt tcatcgtcaa ttaactgatc
     1321 gaacttcgac cacataaatc caggtgcaac tccacctgtt cgtgtgtgac ctcgatgacc
     1381 aaagcttttg ccatcgcgtg ttaaggaaac catggcatcc ggcgcgtacg atccgcgaac
     1441 cggtaaaaca ttgaagggga agtgggtgct aaacgttagc ttgccatatt tagatgtgta
     1501 gttgtggcgc ggtgcgtcgt gttcctgtcc gctgcggcta ttgagcagca gaacctgacc
     1561 ggtgtcctta cggcctgaga cgacatagcc tggtgcaggc aagacaaggt cgtaatcgtc
     1621 tttctcaaca gggagaggtt tctctgttgc ggtccagaac ggatcatcgc ggtccaatgt
     1681 cagcgcgaat aaaccatgac aacaccagta gggggaaccc ggcgaaatat aagcttcacc
     1741 tgccggagga aattcgccat gatagccttg cctcacaaag tgtccatccg aatcaaaaag
     1801 gccgtgatcg tgaaaataac ggatgcaacc gctggagacg cgtcgcaaca gcccgggatc
     1861 ttccggcgcg attttcagaa agtgccctac agcaaatggt gcgagtgctg caaatcgata
     1921 tacgatggag cgtccccagg caggatagga gccattggag ccgaaaaagt gcaggaaacc
     1981 atcaatgaag gaacgccctc gttgccggac gagttcacga tgttcgggac ggcgatcacc
     2041 gtcgatgtat gtccagagga aataatgcca gccaaacatc caggcgttat acaattcaaa
     2101 ttcatctgtc ggaccgtcca cataccagcc atcccctcga tagaattcac caatttggag
     2161 tagccgatag tccaggtcgt ctaccggaga aggaaaaccg agataatggc gtacagcctg
     2221 ggccatcgcg gtgaacagaa tccagttgtc ggtataagtg cctttgttat ctacctgcgc
     2281 gagccatgtc atgatttgct cttgttcagc tttggtcatc ttgttgaaca cacgctcgcg
     2341 actcatccat atcgcaacag caatatccgc actttctaca atgcgttgat ccaggtgggt
     2401 aatatcaccc caatacgtgt acggattcga tgaattcgtc ccatccagca atgcctgccc
     2461 aagaagagtt tcaaggttca tttcatgacc atgaaatttg atcacagcgg gattcgccgg
     2521 attacgcagc caggcacccc aggcagatgc aattcgcgca aaggactcga ttgcgtccac
     2581 cgaatcgggc aaaccgcggc ggtcatccgg ataaagcacg cgcgcaggtg aacccgtctg
     2641 ctcagcaatt ttggcatagc cataggtcat acgcgcaaag atatattccc agtgggcgcg
     2701 cgtccagcct gtgtagggcg aaagctcaaa atcaaagtcc gggaagttct ctgtcatggt
     2761 actcaccgaa acgcgggaaa tacttcctga gacaacagct tcagttcctt cataatatgg
     2821 tcatgcggca tgccagcgaa atagagtctg cagatcagat gatcgacgcc gaaggtgtcg
     2881 acgaagcgtt taatgtgagc aatgcagtca tccgggtttc ctacgataaa acgatcctga
     2941 ctcaacgact ccaatgcaat tgccgtctga tcctgtgagc cgatgagcgg gtgtttccag
     3001 ccgcccccat attcatcgcg ataattcacc atgacatact tttcagccag ttccagcgct
     3061 tcttcgcggg tttccgcaat aaccacttcg cgggtaaggg gattgggtac atctgccgga
     3121 ttctttccgg cgtcggacaa aaacgcgcgg tattgtttct gcgcgttcag gagtttttcc
     3181 aaattggctg tcggacccgg aacccaggca tcgccgagtt gggcggcgcg tttcaatgaa
     3241 agctcacccc aaccaccgag ccagatcggt atcggatttt ctggccgcgg ctcaatggag
     3301 cctttaatct taaagaatcg accgtcatat tcaacacgat cctgagtcca gagggcgcgc
     3361 atgatcttaa gcatttccac ataacgcgcg ccgcggtcat ccaggctgac gtcatacaat
     3421 tcaaactccg gtggacggta tccaatcgca gccccaaaga tgacacgccc atctgaaatg
     3481 acgtccagca tagctgtatc ttctgcgaca cgaactggat gataaaaggg caggacaacg
     3541 atatctgttc caagcaacac tttggatgtc ctggtcgcaa tccctgccag ggccattaaa
     3601 ggtgatggcc aatagtgatt gcggatactg tgatgttcct cgagccaaac cgaatcaaag
     3661 ccaagc
//
LOCUS       DASTJP010000817         6449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1046776, whole genome shotgun sequence.
ACCESSION   DASTJP010000817 DASTJP010000000
VERSION     DASTJP010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046776"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFQ23_23900"
     CDS             complement(<1..395)
                     /locus_tag="VFQ23_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909712.1"
                     /translation="MLDLARRKIRNAIRDVRNGSMLWQKIKHLFRVRAWTSVALQIIL
                     ILVVGNYTAARSDAFLSEFNLNSLLLATIPLALVTMAQMNALVAGYLDISVGSIMTMG
                     VIIASFIITDGAPPNAVLLGGLAILGMGIL"
     gene            complement(398..1981)
                     /locus_tag="VFQ23_23905"
     CDS             complement(398..1981)
                     /locus_tag="VFQ23_23905"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET9909713.1"
                     /translation="MSNSNHDQKFVLELQGVSKQYGTVQALSNVSLDCYAGEVHAVVG
                     ENGSGKSTLLGIASGFVEPDQGVVLIGGKRLRRDSPAEALRLGLGMAYQDNSQVLALS
                     VKENLFMAAPRNLRPAYWKMKKWATDTLAEFELDLFPDAPTMYLTLAQRQLFEVVKAL
                     MGDPKVLLLDEPTTALGPDEVDLLHRMISTRAARGVGIVYVSHRLPEVLTVANRITVL
                     RDGKSQGTYIASEMSEPALVSLMIGRPFHAAFPPALENSAESESVLRINGFQGPLFGP
                     IDLTIKRGEIVGLAGAEGNGQGEFFKCLSGQIPPKAGLVMCDGRELTLISPLDAVQAG
                     ILLLAGNRKHEALFPVLGVRNNATIQVLAKFSSLGWVNRGREQHTVSDLIKQLKVRTP
                     SLEQPVQFLSGGNQQKVSLTRPHLQEIVNVILAYEPTQGVDAGARLDIYEALRAKANE
                     GAAVIVKSSDPIELSGLCDRVIVMSRGQIVDEIQRADLSEKRIIESIVGGVGFGLKSR
                     RARVAGPRAATNLDKDRGD"
     gene            complement(2105..3343)
                     /locus_tag="VFQ23_23910"
     CDS             complement(2105..3343)
                     /locus_tag="VFQ23_23910"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9909714.1"
                     /translation="MKRLFVINMMVILTMALAACGGAAPAPATEAPAAALGPGIEGAD
                     EFGERYDADPAVLTQSVGTPDGVPQIALAALYRAGLPVDQEMQDLAVKCWKEKTCETG
                     TGGPLTVALADGFGENFWRQMTHMEFILQALTYPEIGKIIYVTALGDTQRSISDFRSL
                     IAQDVDIIIGFPDAGDALLPAVREATERDIIYITHSYGAIGAPGTDYPTFVAEDVCLL
                     GQLFAETLNTEVGTGKVAFLGGTPGNALSAYWQSCEEPALSSDLTLVGRADTSWTREG
                     TLEAVSGFISSDPDIKGYSYEAADSFMGGVRAYEAAGLPLDIVVTLRTDEMSLFCEWE
                     KINNPNFKVFYASGGNYQSRIALTAAMMKLKGYELPAEGIILPTVMRELTTGTCNPDV
                     PQEASTSSLIPIPIFQAMGK"
     gene            complement(3423..4469)
                     /locus_tag="VFQ23_23915"
     CDS             complement(3423..4469)
                     /locus_tag="VFQ23_23915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9909715.1"
                     /translation="MKNQIQSQLLTSIVISSRYLSVWIAIGLLLLTALWIAPQTLTRT
                     SFSAVLPLTSFLALAALGQMLVVMTGGIDLSIPGVMTLAAIMAVGISGNSDERLPLAF
                     ASALGVSLLIGFISGVLVGVLKLNPLIVTLAIGQIVYGATLAYADSVPNEASVPPAFA
                     AWINRPLFGLSNLEWTAIGASLLFVVMFRYTEIGRKFQSVGANPHAAWILGLRVNVYV
                     ILAYMVACLLYGLNGILLAGFLRSPSLVLGAPYLLGPIAAVVIGGASLSGGLANAVST
                     WAAAFFLELLSQMLRVLGLPTALQFAVFGLAIIGGMIISGDRIVTLIENSLMGISRFK
                     ARDAEEKKGGAPVA"
     gene            complement(4478..5824)
                     /locus_tag="VFQ23_23920"
     CDS             complement(4478..5824)
                     /locus_tag="VFQ23_23920"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9909716.1"
                     /translation="MTSQRTLIKNGIVLTLDKSVGNFHQADVLIEGTKISAVGPNLGA
                     TDAEVVDASDMIVMPGFIDTHRHVWEGILRNIGTDIPLEGDASYLMVIQNTLAPAYRP
                     EDAYIGNLVGLYGAIDAGITTILDWSNVQATREHTDAVVKALQESGMRAVFAYGYPWW
                     KEPDANQESWIKETAKQYFSSKDQLLTFALAPHGPEFDSFETTKSQWQFARELDARLT
                     VHVGVGTAGKRGKLAEMGKAGLLKNDTTYIHCTTLSDEEIKMIVDTGGTVSLASTNEM
                     SMGYGMPPVQRFLNHGLRPSFSVDTETNTPNDMFTQMRSVNSLQHAMIFDRKLEGKRN
                     LPPFMNSRDVIECATIEGARANGLLHKTGTLTPGKEADLILLRTDRPNILPVNDPIGA
                     VVWGMDTSNVDSVFVAGNALKRNGKLLNVDLDDLRKKAYQSRDYVLAQSGFRLPEI"
     gene            complement(5843..>6449)
                     /locus_tag="VFQ23_23925"
     CDS             complement(5843..>6449)
                     /locus_tag="VFQ23_23925"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9909717.1"
                     /translation="QFGLIGTGVPHGWRNVGDTTARWLEMQSPQPRTMDAQAGRDTFF
                     VAGEVSAQAAPPTSDSQMLGYFDESQLPRPGAPSQMEGFNPTTGVAIKMFVDRSFGAI
                     HQSLFLIQYQPGAKIDSHDHTFEESYFIVGGNVRAIADGQAYDLGPGDVIWTGVGCVH
                     SFSNMGTEPVRWIETQAPLPPAKEIFRFERDWAQYANEQPG"
BASE COUNT         1600 a         1784 c         1593 g         1472 t
ORIGIN      
        1 aggatgccca tgccgagaat ggccagccca ccgagtagca cagcattagg tggtgcacca
       61 tccgtaataa taaaagaagc aatgatgaca cccatcgtca tgatcgagcc aacggaaata
      121 tcaagatagc ctgcgactag cgcgttcatt tgcgccatgg tgacaagcgc caggggaatc
      181 gtcgccagca ataaactatt aagattgaat tcagatagga acgcatcaga tcgtgcagcc
      241 gtatagttgc caacgaccag gatcaaaatg atttgcagcg cgacagaggt ccatgcccga
      301 actctgaaca ggtgtttgat cttttgccac aacatactgc cattgcgaac atctcgaatg
      361 gcatttctta ttttgcgacg ggcaaggtca agcataccta atctcctcga tctttatcca
      421 aattcgtcgc ggcacgcgga cccgcaactc gcgcacggcg cgatttcagc ccaaagccca
      481 ctccaccgac aatggattcg attatccgtt tttcgcttaa gtcggcacgc tgaatctcat
      541 caacgatctg cccgcgcgac atgacgatca cgcggtcaca cagacccgag agttcaattg
      601 ggtcactcga tttcacgatc accgccgcgc cttcgtttgc cttggcacgc aatgcttcgt
      661 aaatgtcaag gcgggcaccc gcatccacgc cttgtgtcgg ttcataggca agtatcacat
      721 tgacaatctc ctgcaggtgg gggcgtgtca acgaaacctt ctgttgattc ccgccagaca
      781 gaaattgcac cggctgttca agggaagggg tacgcacttt gagctgcttg atcaaatcgc
      841 tgacagtatg ttgttcacgc cctcgattca cccagcctag gctgctgaac ttcgccagca
      901 cttgaatcgt ggcattgttc cgcacgccaa ggacgggaaa taacgcttcg tgcttcctat
      961 tccctgccag caacaaaatg cctgcctgca cagcgtccag aggagaaatc aatgtcagct
     1021 ccctgccgtc gcacataacc aatccggctt tgggagggat ttgacctgaa aggcatttga
     1081 aaaattcgcc ctgaccattt ccttccgcgc cagccagacc aacaatctcg ccgcgtttga
     1141 tggtcagatc aatcggacca aataatggac cttgaaaccc gttgatcctt aatacacttt
     1201 ctgactcagc tgagttttcc aacgctggag gaaaagccgc atggaaggga cgcccgatca
     1261 tcaacgatac cagagcaggt tcagacattt ccgaggcaat ataagtcccc tggcttttgc
     1321 catctcgcaa gaccgtgatg cgatttgcca cggtaagtac ctcgggcaaa cgatgactga
     1381 cataaacaat acccacaccg cgcgcggcgc gggtgctaat catgcgatgc aataagtcca
     1441 cttcatcagg accaagagcc gttgtcggct cgtcaagcag aagcactttc gggtcgccca
     1501 tcagggcttt gacaacttca aataactggc gttgtgccag tgtgaggtac attgttggcg
     1561 cgtcagggaa cagatccaat tcaaactcag ctaatgtatc tgttgcccat tttttcatct
     1621 tccaataagc aggccgaaga ttgcgcggtg ccgccatgaa caagttctct ttgaccgata
     1681 aagcgagaac ctgtgaattg tcctgatagg ccatccccag gccgaggcga agcgcctcgg
     1741 ctggggaatc tctacggagt cgtttgccac cgatcagcac cacaccctga tcaggttcca
     1801 caaaaccgct ggcaattccg agaagcgtag acttgcccga accgttctca ccgactacgg
     1861 catgaacctc gcccgcataa caatccaggc tgacattaga caaggcttgc accgtgccat
     1921 attgcttgga gactccctga agttcaagga cgaatttttg atcgtggttc gagttcgaca
     1981 taaatgacgc tttactcaga attattagac ctattgcttc gccacagagt ccattgagac
     2041 ctttgagaaa aactcttttc tcagtgttct ctgtggcaaa gagaattctg caagggcttt
     2101 gcctttactt gcccatcgcc tggaaaatcg ggatcgggat cagcgatgaa gtcgaggctt
     2161 cctgcggcac atccgggttg caagtgcctg tagtaagttc gcgcattacc gtgggaagaa
     2221 taattccttc cgcgggaagc tcatagcctt ttagcttcat catagcggct gtcagcgcaa
     2281 ttcgtgactg gtagttgcca cctgatgcat aaaagacttt gaaattcggg ttgttgatct
     2341 tttcccattc gcagaacaga ctcatttcat cggttcgcaa cgtaacgaca atatcgagcg
     2401 gaagccctgc ggcttcgtag gcgcgtacac cgcccatgaa ggagtcagcg gcttcgtagc
     2461 tatagccctt gatgtctggg tcgctgctga tgaaaccgga gacagcctcc agcgtgcctt
     2521 cgcgcgtcca gctggtatct gcacggccca ccagagtcaa atcggagcta agcgcaggtt
     2581 cctcgcagga ctgccaatag gcagatagcg cgttgcccgg tgttccaccc aggaaggcga
     2641 ccttgccggt tccaacttct gtattcagtg tttcggcaaa gagttgacca agcaagcaga
     2701 catcttcagc cacaaacgtg ggatagtctg tgcctggtgc gccgatcgcg ccgtacgagt
     2761 gcgtgatgta gatgatatcc cgttcggttg cttcacgaac ggctgggagt agagcatcac
     2821 ccgcatccgg gaacccgata atgatatcta catcctgtgc gatcaaactg cggaagtccg
     2881 agatggatct ctgagtatcg ccaagcgcgg tcacatagat gattttgccg atttcggggt
     2941 atgtgagagc ttgcaggatg aattccatat gggtcatctg tcgccagaaa ttttcgccaa
     3001 agccgtcagc caaagcgacg gtaagaggac cgccggtgcc ggtctcgcag gttttttcct
     3061 tccagcattt gacagccaga tcttgcatct cctggtcaac aggcagccct gctcgataca
     3121 gagcagccaa agcgatttgc ggcacgccat ccggcgtacc aacagattgg gtcaggaccg
     3181 cagggtcggc gtcgtagcgt tcaccaaact cgtctgcacc ttcaattccc ggacccagag
     3241 ctgctgctgg agcttccgtt gccggcgcgg gcgctgcccc gccgcatgcg gcaagtgcca
     3301 tcgtcagaat gaccatcata ttgatcacaa ataaacgttt cattttcttc tcctaattgg
     3361 ttggtggata gacaaaaaca attgatgatt cttgaagttt ggctgtgacg cgcggaaatt
     3421 ggtcacgcta ccggggctcc tcctttcttt tcttcagcat ctctcgcttt gaaccgtgaa
     3481 atacccatca gcgaattctc gatcaacgta acaatgcggt ctccagaaat gatcatgcca
     3541 ccgataattg ccagaccaaa gacggcaaac tgcaaagcag tcgggagacc caaaacccgg
     3601 agcatctgac tcaacaactc gaggaagaaa gctgccgccc aggtggaaac ggcattcgcc
     3661 agcccgccag aaagtgaagc cccaccaatc accacggccg caatcggacc caacaaatac
     3721 ggcgcgccca acaccaggct tgggctgcgc aggaagcctg ccaacaagat accgttcaaa
     3781 ccataaagca ggcaggcgac catataggca agaatcacgt acacattgac gcgcaaacct
     3841 agaatccagg ccgcatgcgg gttggctcct acagattgaa acttgcggcc aatttcggta
     3901 tatcgaaaca tcacgacgaa aagaagagat gcgccaatgg cagtccactc aaggttgctt
     3961 aagccgaata agggccgatt gatccaggca gcaaacgctg gcggcacaga ggcctcgttg
     4021 ggcacactgt ctgcatacgc cagggtcgcg ccgtaaacta tctggccgat tgccagcgtc
     4081 acaatcagag gattcaactt cagtacgccc accagaactc ctgatataaa tccaatcagc
     4141 agggaaacgc ccaacgctga tgcaaatgcc aaaggcaaac gctcatccga attgcctgag
     4201 atccctacag ccattatggc ggcaagagtc atcacaccag ggatggataa atcaatcccg
     4261 cccgtcatca cgaccagcat ctgacccagc gccgcgagag cgagaaagga ggtaagagga
     4321 agtaccgccg aaaaagatgt gcgcgtcagt gtttggggtg caatccagag tgctgtaagg
     4381 agaagcagac caattgcaat ccaaacggat agataacgag atgaaataac gatggaggtt
     4441 agaagttgag attgaatttg atttttcatc acagttccta aatttccggc agtctgaagc
     4501 ccgattgagc cagcacataa tcgcgtgatt gataagcttt cttgcggaga tcatccaaat
     4561 ccacattcaa caactttcca tttcgcttga gggcgttgcc tgccacaaag acggagtcta
     4621 cgttgctcgt atccatgccc cacacaactg cgccgattgg atcattcaca ggcagaatgt
     4681 tggggcggtc tgttcttaac aggatgaggt ctgcttcttt acccggagtc agtgtgccgg
     4741 ttttatgcaa caagccattg gcacgcgcgc cctcgatcgt ggcgcattcg atcacatctc
     4801 gcgagttcat gaacggcggc aggttgcgct tgccttccag cttccggtca aagatcatgg
     4861 catgttgcag agaattcacc gaacgcattt gggtgaacat atcgttgggc gtattggtct
     4921 ctgtatccac actgaaactt ggtcgcaaac catgatttag aaatcgctgg accggtggca
     4981 ttccatagcc catgctcatc tcattcgtcg aagccagcga cactgttcca cccgtgtcca
     5041 cgatcatttt gatctcttca tcgctcaagg tcgtgcaatg aatataggtc gtatcatttt
     5101 ttagcaaacc agccttgccc atctcagcca gtttgccccg tttccctgcg gtgcctactc
     5161 ccacatggac agtgaggcgc gcgtcaagtt cacgtgcgaa ttgccattgc gatttcgtcg
     5221 tttcgaacga atcgaactcg ggtccatgtg gcgcaagagc aaatgtcagc aattgatctt
     5281 tcgatgaaaa atactgtttt gccgtctctt tgatccaact ctcctgattc gcatccggtt
     5341 ccttccacca cggatagccg tacgcaaaga ctgcacgcat ccccgactct tgcaaggctt
     5401 tgaccaccgc gtccgtatgt tcacgcgtgg cttgaacgtt tgaccaatcc aggatggttg
     5461 tgattccggc gtcaatggca ccatataacc caacaagatt tccaatatat gcgtcttctg
     5521 gtctataggc tggcgcaagc gtattctgga tgaccatgag ataactcgca tcgccttcca
     5581 gcgggatatc cgttccgatg ttgcggagga tgccttccca gacgtgacgg tgtgtgtcga
     5641 tgaaccccgg catgacaatc atatcggatg catcaaccac ttcagcatct gtcgcgccca
     5701 ggttcggtcc aaccgccgaa atctttgtcc cctcgatcag cacatcggcc tgatgaaagt
     5761 tacccacgct tttatcaagc gtaaggacga tgccgttctt aatcaaagtt cgttgggaag
     5821 tcactctaca ttcctatttg tgttagcccg gttgttcatt cgcgtattgt gcccaatctc
     5881 gttcaaaacg gaaaatttct tttgcaggcg gaagtggggc ttgtgtttca atccaacgca
     5941 ctggttcagt gcccatattc gaaaagctgt ggacgcagcc aacacctgtc caaatcacat
     6001 caccaggacc gagatcgtag gcctggccat ctgcaatcgc ccgcacattt ccaccgacga
     6061 tgaaatagga ttcctcaaag gtatgatcat gcgagtcaat cttcgcgccg ggttgatatt
     6121 ggatcaggaa caacgactga tggatggctc caaaggagcg atcgacaaac attttgatcg
     6181 ccaccccggt cgtcgggtta aatccttcca tttgtgaggg agcacccggg cgaggcaact
     6241 gcgactcgtc gaagtaacca agcatctgtg agtcagaagt gggaggcgcg gcctgtgccg
     6301 atacttctcc ggcgacgaaa aacgtatcgc gtcccgcctg ggcatccatc gtgcgtggtt
     6361 gtggcgattg catttccagc cagcgagccg tcgtgtcgcc cacattcctc caaccatgag
     6421 ggacacccgt gccaatcaat ccaaactgg
//
LOCUS       DASTJP010000818         7163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1451493, whole genome shotgun sequence.
ACCESSION   DASTJP010000818 DASTJP010000000
VERSION     DASTJP010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451493"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFQ23_23930"
     CDS             complement(<1..915)
                     /locus_tag="VFQ23_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9909718.1"
                     /translation="MKKWQLWLGVLISILFIWLALRGLRLEEFWGSVKQANYIWLIPG
                     IAVYFIGVWVRAWRWHYLLGPIKQIPTKTIFPIVTIGYMGNNVYPARAGEVLRAVILK
                     RKEGVSVSASLATIIVERIFDGVVMLGFVFVNLAELAKLTGSSGFVGNIQQVAVIGTG
                     LFLGALVAFLLSAMFPHRTMNIVAWLLDHFAPQRFRKRLTSMTNRFLDGLASLRSPFN
                     VLMVFFTSVIIWLLETGKYWFVMHAFDFSVSFFALMLMNGIVNLATTIPAAPGYIGTF
                     DAPGIAVLTAYGVEHSVAAGYTLVLHVAL"
     gene            985..1488
                     /locus_tag="VFQ23_23935"
     CDS             985..1488
                     /locus_tag="VFQ23_23935"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9909719.1"
                     /translation="MQLNQPALDFELYDLRGKIHKLSDYRGKIVILNFWSAECPHSER
                     TDRVLLDSLVHWGDNVAVLPIASNRNESVQMVKEAAKTRHLPTVLLDAECKVANRYEA
                     ISTPHVFVLDPDGILRYRGAVDDVKFRQPKATRFYLEEAVEALLNNRKPDPEETLAYG
                     CAIVREI"
     gene            1501..3207
                     /locus_tag="VFQ23_23940"
     CDS             1501..3207
                     /locus_tag="VFQ23_23940"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9909720.1"
                     /translation="MAIEIPARIAFLKKIHPFYGLEDDDYERIANELDEMQVPKDGVV
                     YEQGGKADSFYLIYGGSVRIVRRHNNKEIQLAILVKNDYFGEMALVEHRLHSGTVTAM
                     ADTSLLVLSRKDFEKLYKTDPQLKLNLEVAIKSRQLARRVNFKWLRDDEVIYFLARKH
                     RLVLYQKLILPIVALSVPAFFFYAWYAIVGFTIVGIAAWLSLIAVVVWGFWLIIDWGN
                     DYYIVTNQRVVWLEKVVGIYDSRMESPIHTVLSVGVEANQFGRLLDYGNVIVRTFVGR
                     IVFSNVDHPEQAARMIEEYWGRTREAAVGIEKEAMKNALRKRLGIPIPPQPKPFGSVQ
                     EKPPAPKTKPPWWIAMLGLDALKLRLEEGDSIIYRKHWIALIIQAWMPIAGIFAALTL
                     FLNRLWRLAFDPNAVFISFAGGINIDTWALVFSMGMVPFIGWLIYEIADWSNDKFQVT
                     AEQIIDLDKKPFGTETRNAAQLDSILGTEYKRIGILGNLFNYGTVYITVGGSKFAFED
                     VMDPPAVQSDIDRRRMARSAKKKEAEVATERERMAEWLATYHTSSDDFRREMDKRNQK
                     SE"
     gene            3296..3817
                     /gene="def"
                     /locus_tag="VFQ23_23945"
     CDS             3296..3817
                     /gene="def"
                     /locus_tag="VFQ23_23945"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET9909721.1"
                     /translation="MALRKIVTLPEPVLKRKAHAVTKFDKNLQTLIDDMVETMRAAPG
                     VGLAAPQIGLSERIIVIEYTENEEDEDKEDAPKKVWAVLNPEIIQRSEETLFGVEGCL
                     SIPNLIGEVERHAEVQVKGLNRHGKPFKIKAKGWLARIFQHEIDHINGVLFTERATRV
                     WRPTEEVDLEQEV"
     gene            3897..4049
                     /locus_tag="VFQ23_23950"
     CDS             3897..4049
                     /locus_tag="VFQ23_23950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909722.1"
                     /translation="MSRRGRIFFPEQLEGSGVEDTKKWAGWLTFLIDRNECEATSPDR
                     LQKGQG"
     gene            4059..5264
                     /gene="recF"
                     /locus_tag="VFQ23_23955"
     CDS             4059..5264
                     /gene="recF"
                     /locus_tag="VFQ23_23955"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET9909723.1"
                     /translation="MYLKHLSLTNFRNFARLDIDLPTRIVLLTGANAQGKTSVLEAIY
                     FLAAFTSFQTHADRQLINFTEGRKASLAVARLVADYQRGRSRHRLEVRLILEPMGVLN
                     GQRLRKEILLDGVKKPAHEVIGHFNAVVFVPQMSQIIEGSPEERRRYLNLALAQSTPV
                     YARVLSEYNQALTQRNALLKALGERGGNSDQLGVWDEALTRLGAQLILWRIDAVQQIE
                     RLAARVHHELTHGSEILRLSYEPAFDPLPKPERQLGLKIETVIDRSGLTLNEIQDGFL
                     ERLRELRSEEIARGVTTTGPHRDELRFIANDIDLGHYGSRGQIRTALLALKLAEVNWM
                     KERTGEWPVILLDEVMAELDIQRRTDLLKYVGASEQVLFTTTDLNLFASDFVENAEVW
                     KVQDGRVEK"
     gene            5392..6171
                     /locus_tag="VFQ23_23960"
     CDS             5392..6171
                     /locus_tag="VFQ23_23960"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9909724.1"
                     /translation="MNLSDSFIQSALEQLDSPDVIGIGIVGSYARGQEAKYSDVDIDI
                     FVNKLPENELDHYTLRYWDDKLISLKQTLLAEERAALSDPRRAIWAVPGLRGMNIILD
                     KDGSLTALRKAAQEFEWSPLQRAADEFASDALMHCAEEIHKVLNGLAHESESTVLYAV
                     WGLIKNLPQAVFVQKGLMMTSENRCFDLLQDSMGWDSAWTQAFRKGWGLSPEGSHYQD
                     RAAGALALYCITAEMLNELIPEKHRLVVNKTLQLIKEAGYS"
     gene            6168..6851
                     /gene="nth"
                     /locus_tag="VFQ23_23965"
     CDS             6168..6851
                     /gene="nth"
                     /locus_tag="VFQ23_23965"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9909725.1"
                     /translation="MNSLAKRAVKIHERLLAFYGEPIWRNPLPAIDELVSTILSQNTN
                     DINRDRAFNALRAKFATWEEVRDATTNDVTEAIRPAGLANQKGPRIQQVLRSITEERG
                     SLDLSFLADLSVNEARAWLTKFNGVGPKTAAIVLCFSLDMPAFPVDTHVYRVTGRIGL
                     RPEKMTVEQAHPHLESLFPPETYYAAHLNIIRLGREICQARRPRCEVCPIIKLCDYGR
                     NVTSLRGGH"
     gene            complement(6931..>7163)
                     /locus_tag="VFQ23_23970"
     CDS             complement(6931..>7163)
                     /locus_tag="VFQ23_23970"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HET9909726.1"
                     /translation="AGEVAGKIKKVFRDKEGEISQETREALKAELGDVLWYIAQVATE
                     LDLSLDDIAESNIAKLYDRLERGKIKGDGDNR"
BASE COUNT         1875 a         1663 c         1867 g         1758 t
ORIGIN      
        1 caaggcgacg tgcaggacga gtgtatatcc cgccgcgaca gaatgttcca cgccataggc
       61 ggtcagtacc gcaattcccg gtgcgtcgaa ggtgcctatg taaccgggtg cggcggggat
      121 ggtcgtcgca agattaacaa taccgttcat cagcatcagc gcgaagaagg aaacgctgaa
      181 gtcaaaggcg tgcatcacga accagtattt cccggtttcc aaaagccaga taatgactga
      241 tgtaaaaaat accatcaaca cgttgaatgg agaacgcaga gaggcaagcc catccagaaa
      301 acggttggtc atactcgtca gtcttttacg aaatcgctgc ggcgcgaaat gatccagtaa
      361 ccaagctact atattcatag tgcgatgtgg gaacatagca gatagcagaa acgctaccaa
      421 agcgcctaaa aaaagtcctg ttccgatcac agctacctgt tgaatattgc caacgaagcc
      481 tgaactgcct gtcaatttgg cgagctcagc caggttgaca aatacaaagc ccagcatcac
      541 tacaccatcg aaaatgcgtt cgacaatgat tgttgcaagt gaagcagata ccgatacgcc
      601 ttccttgcgt ttgagaatca ccgcgcgcaa cacctcgccc gcgcgggcag gatagacatt
      661 gttccccata tagccaatcg taacaattgg gaaaatggtt ttggtgggga tctgtttaat
      721 gggacccagt agataatgcc atcgccaggc gcgtacccag accccgatga aatacacagc
      781 gattcctgga attagccaaa tataatttgc ctgttttacc gaaccccaga attcctctaa
      841 tcgtagacct cttaacgcca gccagataaa aagaatactg atcaataccc caagccataa
      901 ttgccacttt ttcataggca aggattgtat catcttgcac agacgaaacg aatttcaaat
      961 atctgtcagg cttataattg gaaaatgcaa ttaaaccaac ctgcacttga ttttgaattg
     1021 tatgatctaa gaggcaagat ccataagttg agtgattatc gaggaaagat tgtcattctc
     1081 aacttttggt cagcagagtg cccgcattcc gaaaggacag accgcgtcct gctggattct
     1141 cttgtgcatt ggggagataa cgtggcggtt ctgccgatcg cgtcgaatcg aaacgaatcg
     1201 gttcaaatgg tgaaagaggc tgcaaaaacg cggcaccttc caacggttct gctggacgcg
     1261 gaatgtaagg ttgctaatcg atatgaagcc atttccactc cgcatgtctt tgtacttgat
     1321 cccgatggca ttttgcgtta tcgcggcgca gtggacgatg tcaaattccg tcagcctaaa
     1381 gcaactcgtt tttacctgga ggaagcggtt gaagccctgc ttaataaccg caaaccagac
     1441 ccggaagaaa cgctggctta tgggtgcgca atcgtacgtg aaatttgata gaatcaaatc
     1501 gtggccattg agattcctgc ccgaatcgca tttctgaaga aaatccatcc tttctacggc
     1561 ttggaagatg atgattatga gaggattgcg aacgaactgg acgagatgca ggtccctaaa
     1621 gatggcgtgg tctacgaaca gggcggtaag gcagatagtt tttatttgat ctatggtggg
     1681 agtgtgcgca ttgtccgcag gcacaataat aaagagatcc aactggcgat cttagtaaaa
     1741 aatgattact tcggcgaaat ggctttggtg gagcaccgcc tgcattccgg gacagttacc
     1801 gcaatggctg atacgtcttt gcttgtgcta tctcgcaaag atttcgaaaa attatataaa
     1861 acggatccac agctaaaact aaatcttgag gttgcaatca aaagccgcca gttggcgcgc
     1921 cgtgtgaatt ttaagtggct aagggacgat gaggtgattt attttctagc gcgcaagcat
     1981 cgcttagtgc tgtatcaaaa acttatcttg cctattgtag cgctttctgt cccggcgttt
     2041 ttcttctacg catggtacgc tattgttggt tttacaattg ttggtatagc tgcctggctt
     2101 tcgttaattg cagttgttgt gtggggtttc tggctgatca tcgattgggg gaacgattac
     2161 tatattgtga ccaaccagcg cgtggtttgg cttgaaaagg tggtggggat ctatgatagc
     2221 agaatggaat cgccaatcca taccgttctg tctgtgggtg tggaagcgaa ccaatttgga
     2281 cgccttctgg attatggaaa tgtcatcgtc cgcacctttg tggggagaat tgtctttagc
     2341 aacgtagatc accctgagca agcggcacgc atgatcgagg aatattgggg acggacgagg
     2401 gaggctgcag tagggatcga aaaagaggca atgaaaaatg cccttcgcaa gcgactgggg
     2461 atcccgattc cacctcaacc aaagcctttt ggatcagttc aggaaaagcc tcctgcccca
     2521 aagaccaagc ctccctggtg gatagcaatg ctcggactgg atgcgctcaa actacgtttg
     2581 gaggaagggg atagcattat ctatcggaaa cactggatcg cattgattat tcaggcgtgg
     2641 atgcccatcg ctggcatttt cgctgcactg accttgttcc tcaaccgcct ttggcggttg
     2701 gcgttcgatc caaatgctgt ctttatttct tttgcaggtg ggataaacat tgatacctgg
     2761 gcacttgtat tttccatggg gatggtgccg ttcatcggct ggctgatcta tgaaatcgcg
     2821 gactggagca atgataagtt tcaggttact gccgaacaga tcattgatct ggataaaaaa
     2881 ccctttggca ccgagacgcg caatgctgca caactggata gcatcctggg aactgaatat
     2941 aaacgaatcg gtattttagg aaatctcttc aattatggaa cggtgtatat caccgtgggt
     3001 ggctccaagt ttgcatttga agacgtgatg gacccgccgg ccgttcaatc ggatatcgac
     3061 cgcagacgca tggcacgatc agcaaagaag aaggaagccg aagttgctac cgaacgtgag
     3121 cgcatggcag aatggctggc gacataccat acaagctcag atgattttcg aagggaaatg
     3181 gacaaacgga atcaaaaatc agagtaaaat cgcccgtgct cataccctgc tttgattgtt
     3241 ttcgcaccca gggcatatga gaaaatgaaa ctccatatca aaggtaaacc taatcatggc
     3301 tctccgcaag attgtcacac tccctgaacc tgtattaaaa cgcaaagctc atgctgttac
     3361 aaaatttgac aagaacctcc agactctgat cgacgatatg gttgaaacaa tgcgcgctgc
     3421 acccggcgtt ggcctggccg caccacaaat cggactctcc gagcgcatta ttgtgattga
     3481 gtacaccgaa aatgaagaag atgaagacaa ggaagacgca ccgaaaaaag tgtgggccgt
     3541 cttaaatccg gagattatcc agcgatcgga agagacgtta tttggcgtgg agggctgcct
     3601 ttccatccct aatttgatcg gcgaagtgga acgtcatgcc gaggtacagg tgaagggatt
     3661 gaaccgccat ggcaaaccct ttaagatcaa ggcgaaggga tggctggcac gcatctttca
     3721 gcacgagatc gaccacatca atggtgttct gtttaccgag cgcgccacac gcgtatggag
     3781 acctacagaa gaagttgatc tggaacaaga agtttgatga tgcctttttg ccgatttatg
     3841 ggtaatgacc tgctgtggct ccgtcaattt tgggttgaac agtggacgat aaagcgatga
     3901 gtaggcgtgg cagaatcttt ttccctgaac agcttgaagg ttctggtgtg gaagatacga
     3961 agaaatgggc tggctggctc acattcctga ttgatcgtaa tgaatgtgaa gctacctccc
     4021 cggatcgttt gcaaaaaggg caggggtgat gaagcactat gtatctcaaa catctttcgc
     4081 ttactaactt tcgtaacttt gcccgtctgg atattgattt gcccacgcgg attgtgttac
     4141 ttaccggagc gaatgctcag ggaaagacca gcgtgctgga ggctatttat ttcctggcag
     4201 cctttacttc ctttcaaacc catgccgatc gccaattgat caatttcacg gaaggcagaa
     4261 aagcttcgct ggcggtcgcg cgtttggtag cggactatca acgaggccgg agcagacatc
     4321 gtctggaagt gagattgatc cttgagccca tgggagtgtt gaatggtcag cgcctgcgca
     4381 aagagatcct gctggatggc gtgaaaaaac cggcgcatga ggtgatcggt catttcaatg
     4441 cagtcgtttt tgtgcctcaa atgtcacaga tcattgaagg ctcgccggaa gaaaggcgtc
     4501 gctatctgaa tctcgcgttg gcgcagtcca cgccagtgta cgcccgtgtg ctgagtgaat
     4561 ataaccaggc gctgactcag cgaaacgcgc tgctcaaggc gttgggagaa cgtggaggca
     4621 acagcgacca gctcggagtc tgggatgagg ccttgacgcg ccttggcgca cagctcatcc
     4681 tgtggcgcat tgatgcggtc cagcagatcg agcgactcgc ggcgcgcgtc catcacgagc
     4741 tgactcatgg atctgaaatc ctgcgtcttt cctatgaacc ggcattcgac ccattgccga
     4801 agccggagcg acagttaggg ctgaagattg aaacggtgat cgatcgttct ggtttgacat
     4861 tgaatgaaat tcaggatggt tttctcgaac ggctgcgtga attgagaagt gaggaaattg
     4921 cgcgtggtgt tacgacaacc ggtccgcacc gcgacgagtt gcgttttatc gccaacgata
     4981 ttgacctggg tcattatggc tcgcgcggac aaatccgcac tgcattgctg gcattgaagc
     5041 ttgctgaagt gaactggatg aaggaacgca cgggtgaatg gccggtgatt ttgctggatg
     5101 aagtgatggc ggaactggat atccagcgtc gcaccgattt gctcaagtat gtgggcgcca
     5161 gtgaacaggt cctatttact acgacagatt tgaatctgtt tgcctctgat tttgtggaaa
     5221 acgctgaagt gtggaaagtg caggatggga gagtagagaa gtaataaagg aatggcatgc
     5281 cgagctgcaa ggcggcgagg tatctccttc atttgatgag aactcctcac gtgcactacc
     5341 tccgcagcgt tgtatcagtg tcgctacgct cagagtgaga taaaaattat tatgaaccta
     5401 tcagactcat ttattcaatc tgctcttgaa caactggatt caccagatgt cattgggatt
     5461 ggcatcgtgg gcagttacgc acgtgggcag gaagcaaaat atagcgatgt tgatatcgat
     5521 atttttgtca acaaactgcc tgagaatgaa cttgaccact acacactacg ctactgggac
     5581 gacaaactga tcagcctgaa acaaacgtta cttgcagagg agcgcgctgc attatccgac
     5641 cctcgtcgcg ccatctgggc tgttcctggt ttacgtggaa tgaatatcat tcttgataaa
     5701 gacggctctc tgactgcctt gcgcaaagca gcacaggagt ttgagtggtc tcccttacaa
     5761 cgtgcggcgg atgaatttgc gtcagatgcg ctgatgcact gtgcagaaga aatccacaaa
     5821 gttttgaacg gactcgcgca cgaaagcgag tcaactgtct tatatgcggt gtggggattg
     5881 atcaagaatt taccgcaagc tgtgtttgta caaaaaggct tgatgatgac cagcgagaat
     5941 cgctgctttg atctgctgca ggattcgatg ggctgggatt ccgcatggac acaggccttc
     6001 cgcaagggct ggggactcag cccggagggt tcgcattatc aggaccgcgc ggctggcgct
     6061 ttggctttgt attgcatcac tgcagaaatg ttgaatgaat tgatcccgga aaaacatcgt
     6121 cttgttgtaa acaagacgtt acagctcatc aaagaggctg gctattcatg aactctcttg
     6181 cgaaacgcgc cgtcaaaatc catgaacggc tgcttgcgtt ttacggagag cccatttggc
     6241 gaaatccatt gccggccata gatgaattgg tttcaactat tctctcgcag aacacgaatg
     6301 acatcaaccg agatcgggca ttcaacgcgc tgcgcgcgaa attcgcaacc tgggaggaag
     6361 tgcgcgacgc aacgacaaac gatgtgactg aggcgattcg acctgccggc ctggcaaatc
     6421 aaaaaggacc gcgtatccag caggtgttac gctcaattac agaagaacgc ggaagtttgg
     6481 atctatcgtt tcttgcagac ctgtcagtga atgaagcaag agcatggctg acgaagttca
     6541 atggcgtagg tccaaaaact gctgcgattg tcttgtgttt ttctttggat atgcctgctt
     6601 tccctgtgga tactcacgtt taccgcgtga cgggccgtat cggccttcga cctgaaaaga
     6661 tgaccgttga acaggcgcat ccacatcttg agagtttgtt cccacctgag acctattacg
     6721 cggcccattt gaacataatc cgcctcggac gcgagatctg ccaggcgcgc agaccgcgct
     6781 gcgaggtttg cccaatcatc aaattgtgtg actacgggag aaatgttacg tcattgcgag
     6841 gagggcatta gcccgacgag gcaatcgcct cacgaaacag gagattgcca cgccgtcgga
     6901 aaatcaccga cagctcgcaa tgacagaagg ttatctatta tctccatccc ctttgatttt
     6961 tccccgttca agccggtcat ataacttagc gatatttgat tccgcaatgt cgtccagaga
     7021 taaatccaac tcagttgcga cctgcgcgat ataccataat acgtccccaa gttcagcttt
     7081 gagcgcttcg cgcgtttcct ggcttatctc accctcttta tcgcggaata ctttcttgat
     7141 cttacctgcc acttctcccg cct
//
LOCUS       DASTJP010000819         7906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1135921, whole genome shotgun sequence.
ACCESSION   DASTJP010000819 DASTJP010000000
VERSION     DASTJP010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135921"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2869
                     /locus_tag="VFQ23_23975"
     CDS             <1..2869
                     /locus_tag="VFQ23_23975"
                     /inference="COORDINATES: protein motif:HMM:NF020498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HET9909727.1"
                     /translation="VYAPILSDGNGLLVTTSDGTENALNVYRLQNSSDNLSLVDQVKL
                     DQQPGRIALSILDAQNFVAAIPGTKYIVRMINGQLQQLENEDGRSASIVDLDMISADK
                     GWGKSIDSRCSTSVLDNQATSVSCAANTSLLETNDGGVTWRPVNLPFAVTDRAFYESQ
                     RQTNPRQTSAVANGENTLAVIGQGFDRCEIPTLSQMQTWSQSSPYQSVNLYIGGINRA
                     CSNNPLNASYIFQMSQQGWTFFPTWVGPQAPCANYINRFSSDVTMAFQQGVDQANLAV
                     ERLAELALTGPTKTGSVVYYDMENYGTTTSCRAAVNAFMNGWVSQIRARSNLAGVYGS
                     TLCNTGLSDFQNIANVPDVIWPARWYHNFGEGFYDPNANVWNLGSCLPNTAWANHQRI
                     RQYEGDHNETWGNLTLNIDSNVLDGLVTTSYDYPFVSSIRAADPNPITASSVSFTVTF
                     SKAVSGVNTTDFSLTRTGSIANASITSVTGSGNTYTVIVNTGSGTGTIRLDLLDNDSI
                     KDAGNQSLGGSGMGNGNFTNGESYTISAPPNIDVLIDEELRGQYFIPPNGSTRESYAG
                     LNQGPVRVTGTGTDSMLAALRMIWQEPGYRSSYSEVMGLPKPVEPSTYWTEYWFPWYN
                     NATSSMEQAFRIANIDSSATTIDVSVGTTVIDSFSLGADQSILKSYPGVDNGPIHIQS
                     TNGRKILASLQVIWEEPGFRSSYSELIGLPQEQLSTEYWFPWYNNAVVNSMDQGFRIA
                     NVDTTSGNTVEVWIGTTKLETISLSAGGSIRVGYNIDNGPARVVCITCTSGGNDKIIA
                     ALRVIWKEPGYRSSYSEMIGLPKEQLSTEYWFPWYNNSSYSMDQGFRVANVDTTLHTV
                     KIFVGAAQIGSDLNLTAGGSVRVGYPVNNGPIRVVCTTCNPLTNDDRIIAALRVIWTE
                     PGFRSSYSELMGLPKEQLSTEYWFPWYNNVDLDTQLRFGVP"
     gene            complement(2995..4899)
                     /locus_tag="VFQ23_23980"
     CDS             complement(2995..4899)
                     /locus_tag="VFQ23_23980"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HET9909728.1"
                     /translation="MFFSIQNKHRRAAAGQTRFGTNITLIILILLSLSSMATNSYVAP
                     PPPRTPTPSPTPGIGCTSTADCLTKMTLDEKIGQMTQANKNALTTPSDITTYYLGSLL
                     SGGGEGPNGSGGTAIQWADMYDGFQGYALQTRLRIPLIYGVDAVHGHNNVFGAVIFPH
                     HIGMGATRNIALIQQAEEITRDEVLGTGIDWVFAPCVCVPRDDRWGRTYEGYSEDPAL
                     VASMGVASIKGFQGIALGPSTVLATAKHYVGDGGTSFGTGDSGYLIDQGNTQLAEADL
                     RSIHLPPFQAAVQNGVGSVMISYSSWNGQKLHGHQYLITTVLKGELGFDGIVISDWAG
                     IDQIPGDYASDVRTAINAGIDMVMVPSNYRTFISTLRTEVTAGRISISRIDDAVTRIL
                     NAKFALGLFSQPLTDRSYTSQVGSPAHRAVARQAVRESLVLLKNTGVLPLSKSDTYKI
                     VVGGSHIDNLGYQMGGWSISWQGAGGNPSVGTTLLQAIQAAVGPSVTVQNVGTRTKGS
                     YSGDVGIVAIGETPYAEGRGDSSTLAVSGSNAAQVTDICSRVTRCVVILFSGRPLIIN
                     TQLNQSHGFVAAWLPGTEGSGITDVLFGDYPFVGKLPITWPNAVTQEPINIGDGKTGL
                     FPFGFGITPY"
     gene            complement(5243..6931)
                     /locus_tag="VFQ23_23985"
     CDS             complement(5243..6931)
                     /locus_tag="VFQ23_23985"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET9909729.1"
                     /translation="MARKLLSVFITILLASLVMVSPALAAKSYYAEYFDVQIDLQQGG
                     SAIVTETVKFHFSGDPFTFAFREISARETDGVTFLDATLDGVPMPQGTQAGQVEVEAG
                     DPLKVTWHFPPASNTSHEFVVRYRVDGVIRTGNGDTLIWRAVPEEHEYLIERSTITLI
                     YPPEVTTLAQPTLDWNHDASFEENRVILTTNGIGEDQDVILSANFAPNSLTQTAPQWQ
                     VKSQQAAATRARALPAGLLAGIATLIVGGLGLFTYLRANGRDLNISSVVPTAIPPSNL
                     SPAIIGKLTGQSHTAMGAIFDLAQRGVLEIREEKGYWGTQNHVLFLKDRSIPLQPFEQ
                     GLLDALIKAGEWQVNMNEVGGRLATKNKLFDAPLEQELVQRGWLDPERKQKRTFFLGI
                     GITALLIALGVFLAALLLGLNSSQNVNLAIAFAVLGGISLAVFLLSLVVLIYAGSFSI
                     LTPAGEEQSARWKGFAEYLKQVSKGREPAIRPDYFEKYLAYASVFGLGANWAKYFQKL
                     GDVPLPIWFHALDAKNANFGAMVAVMSTSDSTGAGGGGAAGGAGASGGGASGAG"
     gene            7071..7808
                     /locus_tag="VFQ23_23990"
     CDS             7071..7808
                     /locus_tag="VFQ23_23990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9909730.1"
                     /translation="MFLRISIFYVLTWFFLILIGGIQQATGLLSPEVGLPQLGPGIAG
                     MLMLLIFRKDGHRIQFLSKETPFSRYLYAALIPVGLGLVVFLISSVIDIPPSAASPIY
                     NSLITAILWMPIGALGEEIGWRGYLHKKLDTKMRGLFSSLLVGLLWLPIHITFLTQGA
                     VFVFFLGVLIISYSIVIYVLVQDTRFNVLLASIFHFAINLTNLLFLDVIYDLTFMIIN
                     SLVWAVVATGLVISNRELFLASKGRVP"
BASE COUNT         1978 a         2035 c         1960 g         1933 t
ORIGIN      
        1 agtttacgca cccatccttt ccgatggaaa cggcctgttg gtaactacaa gcgacggaac
       61 cgaaaatgca ctgaacgttt acaggctaca gaattcctct gacaatttat ccctggtgga
      121 tcaagtaaag ctggatcaac aacccggaag gattgcgctt tcgattctgg atgcgcagaa
      181 cttcgtcgct gccattcccg gaacgaaata catcgttcgt atgataaatg gacaacttca
      241 gcagctggaa aacgaagatg gacgttctgc ctcgatcgtt gatttagata tgatttcagc
      301 ggataagggc tggggaaaat cgattgactc acgctgctcc acttcagtct tagacaatca
      361 ggctacttcc gtttcctgcg cagctaatac gagtctattg gagacgaacg acggtggagt
      421 cacctggcga cctgttaatc ttcctttcgc cgtgacggat cgcgcgttct atgaatcgca
      481 gcgccagaca aatccacgcc agacgagcgc ggtggcaaac ggcgaaaaca cgctggcagt
      541 cattggacag gggtttgatc gctgtgaaat ccctaccctg tcacaaatgc agacctggtc
      601 acaaagcagc ccatatcaat ctgtgaatct atacatcggt ggaatcaatc gcgcttgttc
      661 aaacaaccct ttgaatgcat cctatatctt tcagatgtcg caacagggct ggacgttttt
      721 ccccacctgg gtaggtccgc aggctccgtg tgccaactat atcaaccgct ttagcagtga
      781 tgtgacgatg gcatttcagc agggagtgga tcaggcaaac ctggcagtag aacgcctggc
      841 agagctggct ttaacaggtc cgacaaagac cggctcggtc gtttactatg acatggaaaa
      901 ttacgggacg actacctcct gtcgtgccgc cgtcaatgcc ttcatgaatg gatgggtctc
      961 acaaattcgt gctcgcagca atttggcagg tgtttacggt tccaccttgt gtaatacggg
     1021 cttgagcgat tttcaaaata tcgctaatgt gcccgatgtc atttggcccg caaggtggta
     1081 tcacaacttt ggcgaagggt tctatgaccc caatgccaat gtgtggaacc ttgggagttg
     1141 tcttcctaac acagcctggg cgaaccatca gcgcatccgc caatatgaag gggatcacaa
     1201 tgaaacctgg ggaaacctga cgctgaacat cgatagcaac gttctggacg gcctggtaac
     1261 aacttcgtat gattatccgt tcgtaagcag tatccgcgct gcggatccaa accctataac
     1321 tgcaagcagc gttagtttta cggttacgtt ttcaaaagcg gtttcagggg tcaatacaac
     1381 cgacttttct ctgacaagga ccggcagcat cgccaacgca tcgatcacaa gcgtgacggg
     1441 ttcggggaat acttatacgg tcatagtaaa tactggttct ggaacaggca cgatccgcct
     1501 ggatcttctc gataatgaca gcatcaagga tgcaggcaat caatctttgg gcggaagtgg
     1561 aatgggcaac ggaaatttca caaatggcga aagctacaca atttcagctc cacccaatat
     1621 tgatgttctt atcgatgaag aattgagagg tcaatatttc atcccgccaa atggcagtac
     1681 acgcgagagc tatgccggtc tcaatcaggg tccggtgagg gttaccggga ccggtacaga
     1741 ttcgatgtta gccgccctgc gaatgatctg gcaggagccg ggttatcgca gtagctattc
     1801 ggaagtgatg ggcctgccca agccagtcga accatcgacc tactggactg agtattggtt
     1861 tccctggtac aacaatgcca cttcttccat ggagcaggca ttcagaatag caaacataga
     1921 ctcctcagcg acgaccatcg atgtttctgt gggcacaacg gtaatcgata gcttttcgtt
     1981 aggagcggat caaagtatcc tgaaaagtta tccgggtgtc gataatggtc ccattcacat
     2041 ccagagcacc aatggcagga agatactcgc ttcgctccag gtcatctggg aggaacctgg
     2101 ctttcgcagt tcgtattctg agttgatcgg gttaccccag gagcagcttt ccaccgagta
     2161 ttggttcccc tggtataaca atgcggttgt caactctatg gatcagggct tccgcatcgc
     2221 caatgtagat acgacctcag gcaacacggt ggaggtctgg ataggtacta cgaaactgga
     2281 aacgatttcg ctctctgcag gtggcagtat ccgagtggga tataacatag acaacggccc
     2341 ggctcgcgtg gtttgtatca cctgcacgag cggaggcaac gacaagatca tcgccgcgct
     2401 gcgcgtcatc tggaaggagc cggggtatcg aagcagctac tctgaaatga taggtttacc
     2461 aaaagaacag ctttccactg aatattggtt cccgtggtac aacaattctt cttactcgat
     2521 ggatcaaggc ttccgcgtcg cgaatgtgga taccaccctg cataccgtca aaatatttgt
     2581 cggcgcggcg caaataggct ctgatctcaa tctcaccgcc ggaggcagcg tgcgggttgg
     2641 gtatcctgtg aataacggtc ccattcgcgt cgtttgtacc acctgtaacc cgctcactaa
     2701 cgatgaccga atcatcgctg ccctgcgcgt gatctggacg gagcctggct tccgcagcag
     2761 ctactcggaa ttgatgggct tgcccaaaga gcagctatct accgaatact ggttcccctg
     2821 gtacaacaat gtggatctcg acacccagtt aagattcggc gtgccatagt atctgcctaa
     2881 caatctagat tttcgtaaag tatcccgata cttcaaacaa acaggtctcc tcggagacct
     2941 gtttgttgat ttgcgtatga ctatctcaaa gctacttgcc tttggaaatg atcgttaata
     3001 cggtgtgatt ccaaatccaa atgggaacaa gcctgttttg ccatcgccaa tattgattgg
     3061 ttcctgtgtg accgcgtttg gccaggtgat aggtagcttg ccgacaaagg ggtaatcacc
     3121 gaagagtacg tcagtgattc cggacccttc agttccggga agccaggcag ccacaaatcc
     3181 atgagactgg ttcaactgag tattaataat caacggacga cccgagaata gaatcaccac
     3241 acaccgagta acgcgtgaac agatatcggt cacctgggcc gcatttgaac cgctcaccgc
     3301 cagagtagag ctatcccctc tgccttccgc gtagggagtt tcaccgattg caacaatacc
     3361 cacgtcaccg ctgtaactgc ctttggtgcg ggtgcctacg ttctgaacag tgaccgatgg
     3421 tccgacggct gcctggattg cttgcaatag tgtagtaccg acgctcgggt ttccacctgc
     3481 tccctgccag ctaattgacc aacctcccat ttgatagccc aggttatcga tatggcttcc
     3541 gccgacaacg attttatagg tatcagactt ggacaacggt agcacacccg tatttttcag
     3601 cagcaccagc gattcacgga ctgcctgacg agccactgcc cgatgagcag gcgaaccaac
     3661 ctgagaggta tacgaacgat ctgtaagggg ctggctgaac aaccccaagg caaacttggc
     3721 attgagaatc ctggtcactg catcatcaat acgggagata gaaatacgac ctgcagtcac
     3781 ttccgtgcgc aatgtactga taaacgtcct gtagttggag gggaccatca ccatatcaat
     3841 gccagcattg atcgctgtgc gtacatcact ggcataatcg cctggaattt ggtcaatccc
     3901 tgcccagtcc gaaatgacaa ttccgtcaaa acccagttcg ccttttagta cagtcgtaat
     3961 gagatactga tgaccatgta gcttttgtcc attccagctg ctataggaaa tcatcacaga
     4021 accaacacca ttttggacgg ccgcctgaaa aggtggcaaa tggatcgatc gcaaatctgc
     4081 ttcagcgagt tgtgtatttc cctgatcgat aagataaccg ctatcacctg ttccaaacga
     4141 ggtgccgcca tcgcccacgt agtgcttggc tgttgccaac acggtggaag gacccagggc
     4201 gatgccctgg aatcccttga tagaagcaac gcccatcgaa gcgaccagag ccgggtcttc
     4261 gctataacct tcgtacgtgc gaccccaacg gtcatcacga gggacgcaga cacatggggc
     4321 aaacacccag tcgattccag tgcccagcac ttcatcgcgc gtgatctctt ccgcctgctg
     4381 gatgagagca atattgcgcg tggcacccat gccgatatga tgaggaaaaa tgactgcacc
     4441 aaacacgttg ttatgcccat ggacagcatc cactccatag atcagaggaa tccgtagtcg
     4501 cgtctgcaaa gcatagccct ggaagccatc gtacatatct gcccactgta tagccgtgcc
     4561 gcccgatcca tttggtcctt cgccaccgcc actgagtagt gagccaagat aatacgttgt
     4621 aatatcagag ggcgttgtca acgcgttctt gtttgcctgt gtcatctgac cgatcttctc
     4681 atccagtgtc atcttagtga gacaatcggc agtggaggta cagccaattc ctggggttgg
     4741 ggagggtgtc ggagtccgcg ggggtggcgg tgctacgtag gagtttgttg ccatggagga
     4801 caaagataga agtatgagaa tgataagggt gatattcgtt ccaaacctgg tttgacccgc
     4861 tgctgcccgc cgatgtttat tctgaattga aaaaaacatg ggaactcctc tcgaaagtaa
     4921 gtttgaactt agttattata agcctccttc ttccgttatt gattcgaccg aggctcaacg
     4981 gatcataaat actgaaggaa tccccgcaag aattaaaaag aagcgagacc tcgctagcga
     5041 gatctcgcta tttctgcaat acaccggttt attatgtttt agaggccaca ccggacgcga
     5101 accggtaaaa ctcccgtttc cagtgggatg cacaacccct gtgctgggtg gcctatgagt
     5161 aacaaccctt tactagacca ccaggctaag cccaagaaca ttgcgaacca attacaagtg
     5221 agtctctacg tgtggttatg aactacccgg ctccactcgc gcctccacca gaagcgccgg
     5281 ccccaccggc tgccccaccg ccacctgctc cggtcgaatc agaggtggac atgactgcaa
     5341 ccattgcacc aaagttcgca ttcttggcat ccagggcatg gaaccagatt ggcaacggca
     5401 catcgccaag cttttgaaaa tacttcgccc agtttgcacc cagaccaaat acagatgcat
     5461 aggctagata cttttcgaaa taatccgggc ggatcgccgg ttcccggcct ttgctcacct
     5521 gtttaagata ctctgcaaag cctttccaac gcgccgattg ctcttcaccg gcaggagtca
     5581 atattgagaa agacccggca tagatcaaaa cgaccaggga aaggagaaac acggcaaggc
     5641 ttatccctcc cagcacggca aaggctattg cgagattaac gttctgactc gaatttaggc
     5701 ccaaaaggag tgcggcaaga aaaacaccca acgcaatgag cagggctgta attccaattc
     5761 cgagaaagaa tgttcgtttc tgcttacgct ccgggtcaag ccagcctcgc tgaacgagct
     5821 cctgttctaa tggcgcatcg aaaagtttat tctttgtcgc caggcggcca ccgacctcat
     5881 tcatgttgac ttgccattcg ccggccttga tcagcgcatc gaggagtccc tgttcaaagg
     5941 gctgcagcgg aatagagcgg tccttcagaa acaacacatg gttttgcgtt ccccaatatc
     6001 ccttttcctc gcgaatttcc agcacgccgc gctgagcgag gtcaaagatg gctcccatgg
     6061 cggtatgcga ttggccggtc agctttccga tgatggcggg agataaattc gaaggcggaa
     6121 tggctgtggg aacgacagag ctgatgttca agtctcgtcc gttggcacgc aggtaggtga
     6181 atagccctaa tcctccaacg atcaacgtcg caatacccgc caaaaggccc gcaggtaatg
     6241 ctctggctct cgtggcggcg gcttgttggc tttttacctg ccattgaggc gcagtttgag
     6301 tgagactgtt cggcgcaaaa ttggcactca ggattacatc ctggtcctca ccaattccgt
     6361 tcgtcgtgag aatcacgcgg ttctcttcga aggaggcatc atggttccaa tcgagcgtgg
     6421 gttgtgcgag agtagtaact tctggcggat atattaatgt gatagtagag cgttcgatca
     6481 aatattcatg ctcctccggc accgcgcgcc agattaaggt atcgccgttc ccggtacgaa
     6541 tgactccatc cactcgatat cggacgacaa actcatgtga ggtgttcgac gcaggaggga
     6601 aatgccacgt taccttgagt gggtcacctg cttccacctc gacctgcccg gcttgtgtgc
     6661 cttgcggcat cggaacaccg tccaaagtgg catccaggaa ggtcacaccg tcagtttccc
     6721 gagctgaaat ttcacgaaac gcaaaggtga aggggtctcc tgaaaaatgg aatttcacag
     6781 tctctgtaac gatcgcagaa ccgccctgtt gcagatcaat ttgaacatca aagtattctg
     6841 catagtagga cttggcagcc aatgccgggc taaccataac tagcgaagct aacagaatag
     6901 tgataaaaac acttaaaagt tttcgggcca tttgttcctc caaatacgtc tacgtatagt
     6961 gtatctaaaa gttgaccgtc tgaccgtgtg tttaacaaga ccttaacaat cgaaaatcaa
     7021 agagattttt tcgccggagt gtatgattga agggtcttta aggagaacct atgtttctgc
     7081 gcatcagtat attctacgta ctaacctggt tctttctgat cctgattggt ggaattcaac
     7141 aggccacagg tctactctcg ccagaagtgg gacttcccca attgggtccg gggatcgccg
     7201 ggatgttaat gcttcttatt tttcggaaag atggccatcg gattcaattc ctctcaaaag
     7261 agacaccttt ctctcgttac ctgtacgccg ctttgattcc tgtcgggtta ggtttggttg
     7321 tattcctgat cagctctgta attgatattc caccctctgc cgcttcaccg atctataact
     7381 ctctgataac agccatctta tggatgccaa ttggagctct cggtgaagag atcggatggc
     7441 gtggttatct gcacaaaaag ttggacacga aaatgcgagg cttgttttcg tcgttgctcg
     7501 ttggtcttct gtggctgccc attcacatca catttcttac gcagggtgcg gtcttcgttt
     7561 tctttctggg ggtgttgatc atttcctact ccatcgtgat atatgtgctg gtgcaggaca
     7621 cccgttttaa tgtgttactc gccagcattt ttcatttcgc gattaacctg accaatctgc
     7681 tgtttctgga tgtgatttat gatctaacct tcatgatcat caacagcctc gtttgggcag
     7741 ttgtggcaac aggtcttgtc atttcgaaca gagagctgtt tctcgcatca aaaggcagag
     7801 tcccgtgatg tagaagtcat cgacctgtta actatgtact gagtccaggc tggcacaagt
     7861 gaattaccaa aagaccaaag ccaatgcagc gttggtctgc gagtac
//
LOCUS       DASTJP010000820         6362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1506824, whole genome shotgun sequence.
ACCESSION   DASTJP010000820 DASTJP010000000
VERSION     DASTJP010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506824"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="VFQ23_23995"
     CDS             complement(<1..1692)
                     /locus_tag="VFQ23_23995"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9909731.1"
                     /translation="MLETLNSYVPILIRRRFARSPEPLHAAEYEALEAAVLYADISGF
                     TRLTEELVRTGPRGVEKISIGLNDYFGRWIDIISRYGGDVVKFAGDALLAIWPADEKT
                     GGLGEATLQAATCALEAHTNLRGYQTAEGNPLVIRTGISAGRIRAVHLGGLLKRWELL
                     IVGKAISEVSITMQKATPGTVVLSPTALEHLGSNAQGRRIADGYFTLLNLESRIRNFD
                     SPQKLFQTPHEGSAALETYIPGAIRARLAAGQIGWLAELRTIATLFMNFPDLNSSTNL
                     ETAQEVMVQVQKALYYYEGSVNDSFVDEKGSTLLTAFGLPPFSHEDDALRATLTALEV
                     QQRMQEMGLRLNVGIATGRAFCGSIGNDSRRKYTMIGDIVNLSARLAQAARQMTETES
                     LILVDEATMESTRHRVQFETLPPVHVKGRSEAIAIFTPKKEQRQASHISVEMVGRHKE
                     RRQLTQAMQELTNLEAVNAARIFLIEGEPGIGKSRLLTEALSQAQQIGLLSLAGTADA
                     IEKSTPYFAWRHIFAQLFGLDFTTSMSGRKEALVSTLDDDLVERAALLNDILQVDF"
     gene            1940..2170
                     /locus_tag="VFQ23_24000"
     CDS             1940..2170
                     /locus_tag="VFQ23_24000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909732.1"
                     /translation="MNTVYEDDELGQDTLSIMDTLQTLDPLTYMPSRAANYPDSEFGL
                     ALKQTTILNNRTHAESHERFGRWARSIPRGHV"
     gene            2163..2480
                     /locus_tag="VFQ23_24005"
     CDS             2163..2480
                     /locus_tag="VFQ23_24005"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HET9909733.1"
                     /translation="MFDHMNSLTTVTMSEFGRRASENGSLGTDHGHEGMMMVLGGHVH
                     GGKVHGEWPGLEDGQMVGPGDLAVTTDYRDVLSEVLTKRLNNTATNQIFPNDQAQETK
                     TFA"
     gene            2640..6254
                     /locus_tag="VFQ23_24010"
     CDS             2640..6254
                     /locus_tag="VFQ23_24010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909734.1"
                     /translation="MAEKIYFQWQNPVLRKTIYPRRDLKLQHFLLYYKEIDLWAEHRN
                     KKIEDFSKDKEEFEKNQKAEILAAYQSEQLLRDYFKKPMKPSVPETASKRGKDEMDRF
                     ANLIAGMHTAFGENFVNYEQEEDESRDRKEKYFVTQRLEEWDGYLNDLRRTIVKNISK
                     DLNALEFLEKTLLPIFEVEHDNLKAFVSAQAKLEKRKAEFAKRKKENTRKAAEINRVL
                     EPLQKQMEIWGAELGKITATIARLTNPRKVEDVLEYLKQDLYVKIQRRFEEGQWKVEE
                     SAIQIIKNTVHTAFDDVIESLQKGILPFIGTSDPGVLKQLVKSQSEKTRKAQKQLDKE
                     INKLDSKIESLRILLEEKMSSSPDDLQGIRSLEDKKRIHQNASSILDGSGMREILQGE
                     VDTLDDLYVAIDFRGHDKAKWNEKIKDEEAKLEKLQKNIAKNEPDVAKSLKELEPILS
                     VLEIGREKFLSEFILDREIDVRDIVLYLTEKERARLEGLTSDELLNEVFQRFWKEPER
                     FPLWLQYMVIHFSGMRYASAHDSWYDPRALLLDLWTMDIQEGLKNNDDDPSNDTDYKI
                     ESLTDEQVLEKLKTYKSQLPKWMWKEIVEVTDLRLTEVTNPDEDWETLSSDEQKERNS
                     ARDKKTGQYLKIMEKWKKNKMTGWRKEHDRANRLVVTRAVCNEVAEHIQHLRGHEGAA
                     GLAEKPNWYMGEESKYYESKERKPGSRPYFVKAKGRNDFEVGASILWLRFVHESPDQW
                     RKAKALETRAGDGLIPQAYLGRSIDSGSWAYKMDDPIVRTRIKATTNNTKGSNKGSRG
                     GKENQWLRWMHEATVVEVAETAETKGENRVVLTFETDLPSEDPSRSTMGLVKRYLSDL
                     TREQVEDSYNAAFVGYMPEAKLGADAAVGLASDLEVMLDWNHILQREVVSAAEMETYR
                     SKYIRNRKPPKPTIEIIDTPERVKGKADLPICEFVKWTDSLATKDNNLWKVRIWGDDK
                     LKLVGLTTTSAAVQEKAPSNFQAVGLYDKETRQRGAVSNFLKIERDEIDELIAMQIED
                     EFKRKQSEPKRSSRKQLEIWRQQKMRWLCKGEGTIYFTKGGKGWENASFILWGTVSLG
                     GNLVQVEGEEEIEFKTAEGKKKMRMARLAGFTKSHWSWPLDELLAQGLVHRCFCALPG
                     DKPTDTPQGIVYSPFFSPRNRDFSGQAKPSAFYLPFDQLVKPDDKDYVGIRQRLKCKE
                     "
BASE COUNT         1804 a         1429 c         1651 g         1478 t
ORIGIN      
        1 aaaatccacc tgcaggatgt cgttcaggag agcggcgcgc tcaacgagat cgtcatccaa
       61 tgtagagaca agcgcctctt tgcgtccgga catggatgtg gtgaaatcca gcccaaaaag
      121 ctgcgcaaag atatgacgcc atgcaaaata gggtgtggat ttttcaatgg catctgccgt
      181 accagcgagg ctcaacagac caatttgctg ggcctgagac aaggcttctg tcaacagtcg
      241 agatttacct atccctggct cgccttcgat aaggaagata cgtgctgcgt tgacggcttc
      301 caggttcgtc agttcctgca tggcttgtgt gagttgtcta cgttctttgt gacgcccgac
      361 catctccacg ctgatatgac tggcctgtct ctgttccttc tttggggtaa agatggcaat
      421 tgcttcgctt ctgcctttga cgtggacggg tggcagcgtc tcaaactgga ctcgatggcg
      481 agtgctttcc atcgttgcct catccaccag gatcagggat tcagtttctg tcatttgccg
      541 ggcggcttgt gccagtcttg ccgaaaggtt gacgatgtca ccgatcatgg tgtatttgcg
      601 ccttgaatca ttgccgatcg agccgcagaa cgcgcgtccg gtcgcgatgc ccacgttcag
      661 gcgcagtccc atttcctgca ttctttgctg gacttcaagt gccgtcaacg tagcacggag
      721 cgcgtcatct tcatgggaga agggaggcaa tccaaacgca gtcaacaggg ttgagccctt
      781 ttcatccacg aagctatcgt tcacgctccc ttcgtaatag tacagagcct tttggacctg
      841 caccatcact tcctgggctg tctccagatt ggtggatgag ttgaggtctg ggaagttcat
      901 aaaaagtgtg gcaatcgttc gcagttctgc cagccagccg atttgcccgg cggccaggcg
      961 cgcacggata gcgcccggaa tataggtttc aagcgcggca cttccctcgt gcggcgtctg
     1021 aaacaatttt tgaggcgagt caaaattccg tatcctgctt tccagattca acagggtgaa
     1081 atagccatct gcaattcgtc tcccttgtgc attgctgcca agatgttcga gcgccgtcgg
     1141 ggaaagaacg actgtgccgg gtgtggcttt ctgcatagta atgcttactt cgctgatcgc
     1201 ttttccgaca atcagcaatt cccaccgttt gagcaaccct cccaaatgga cagcccgaat
     1261 cctccctgcc gagatccccg tacgaatgac cagcggattg ccttctgcag tctggtagcc
     1321 gcgcaagttg gtatgggcct caagggcgca ggttgctgcc tgcaaagtgg cttcgcccag
     1381 tcctccggtt ttttcatctg caggccagat tgccaggagt gcatcccctg caaatttgac
     1441 tacatctccc ccatatctac tgatgatatc gatccagcgc ccgaaataat cgttgagccc
     1501 aatcgagatt ttctccactc cccgcggccc cgtacggacc agttcctccg tgaggcgtgt
     1561 aaaaccggat atgtcagcat acaagaccgc agcctcaagc gcttcgtact cagcggcgtg
     1621 taagggctcg ggtgatcttg caaaccttcg acggatcagg atgggcacat aactgttcag
     1681 agtttcgagc ataagactat ttgcggcgag ttgtcagcca gtcaaggaca caagtcatcg
     1741 atgatttctt caatgcggga ttgtgagaaa aggctacaga gatgattata actgcacgtc
     1801 acgaaagcga atcatcaaca cacctgttcc tgacaggtga attcttttgc ttcttcgggt
     1861 ttcacccttc attgaatgca ttattaggtg tatagcactc aaaatagctt gccattatcc
     1921 acgcctgcgg cgcgtcgctt tgaatacagt ctacgaggac gatgaattag gccaggatac
     1981 tctctcgatc atggatacgc tgcaaactct tgacccgttg acctatatgc cctcacgtgc
     2041 cgcgaactac cccgactcag aatttggact ggcactcaag cagaccacca tactcaacaa
     2101 ccggactcat gccgaatctc atgaaagatt tggccgatgg gctcgcagca ttccacgcgg
     2161 acatgtttga tcatatgaac agccttacga ccgtaacgat gtctgaattt ggacggcgtg
     2221 cctccgagaa cggaagcctc ggcacagacc atggacatga aggtatgatg atggtcctgg
     2281 gtgggcatgt gcatggcggc aaagtccatg gggagtggcc agggctggaa gatgggcaga
     2341 tggtgggtcc gggcgacctg gcagtcacga cggattaccg cgatgtctta tcggaggtgt
     2401 taacgaagcg attgaataat accgcgacga atcaaatctt tccgaatgat caagctcagg
     2461 agacaaagac atttgcataa cctgacttcc aaggtcttta ttctttcacg atttgcagag
     2521 ctgctgatgc gctacccatc agtagctctt ttttacaagc agcttgattt ccctctccaa
     2581 ctttcaaaga atggaataga ataggaatat gatttagtca gttacttaag gagcggagaa
     2641 tggctgaaaa aatttatttt caatggcaaa accctgtctt aaggaaaacg atttatcctc
     2701 ggcgcgattt gaaattgcag cattttctcc tgtattacaa ggaaattgat ttatgggcgg
     2761 agcataggaa taaaaaaatc gaagattttt cgaaagacaa agaagagttt gaaaagaatc
     2821 aaaaagccga gatcttagca gcgtatcaat ctgaacaatt gttgcgcgac tactttaaaa
     2881 aacccatgaa accctcggtc ccagagacag cctccaaacg aggcaaagac gaaatggatc
     2941 ggttcgcaaa tttgattgcc ggaatgcata ctgccttcgg ggaaaatttt gtcaattatg
     3001 agcaggaaga agatgaaagc cgcgatcgta aagagaagta ttttgttacc cagcgcctcg
     3061 aagaatggga tggatatctt aacgatttaa gacgaacgat cgtaaaaaat atttcgaagg
     3121 atttaaatgc actggagttt ctcgaaaaaa ctctattacc tatattcgag gtggagcatg
     3181 ataacttaaa ggcatttgtc tctgcgcagg caaagcttga gaaacgcaaa gccgagtttg
     3241 caaaacgaaa aaaggaaaac acaaggaagg cagccgagat caatcgcgtc ttagaaccac
     3301 ttcagaaaca aatggagata tggggagcag agttgggaaa gattacggca acgattgcac
     3361 gcctcaccaa cccgcgaaag gtcgaagatg ttttggaata tctaaagcag gatctttacg
     3421 ttaagatcca acgccgcttt gaagaggggc aatggaaggt ggaagagagt gctatacaaa
     3481 taattaagaa caccgtgcac acagcattcg acgatgtgat cgagagcttg caaaaaggaa
     3541 ttctgccatt cataggtacg tcagaccctg gcgtgttaaa acaattggtc aaaagtcaat
     3601 ctgagaaaac gaggaaagct caaaaacagc ttgacaaaga aatcaataaa ttagactcga
     3661 aaattgaaag tctccgcatt ttactggagg agaaaatgag ctcctcgcca gacgatttgc
     3721 aggggattcg ctcattggaa gataaaaaac ggattcatca aaacgccagc agtatcttgg
     3781 atggctctgg catgcgcgag attcttcaag gcgaggtaga tacactggat gatctgtatg
     3841 tcgcaatcga ctttcgagga catgacaaag ccaagtggaa tgagaagata aaagacgagg
     3901 aagcaaaact ggaaaaactt cagaaaaata ttgcaaagaa tgaacctgac gtagccaaaa
     3961 gtctgaaaga gcttgagcct atactatcgg tcctggaaat tggaagagaa aaatttttat
     4021 cggagtttat tcttgaccgg gagatcgatg tacgagacat tgttctctat ctgacagaga
     4081 aggaaagggc gcggctggag ggcctgactt cggatgagct gttgaatgaa gtattccagc
     4141 gcttctggaa ggaaccggag cgtttcccgc tctggctgca atatatggtg atccactttt
     4201 cggggatgcg ttatgccagc gcccatgatt cctggtatga cccaagagcc ttgctcctgg
     4261 acctgtggac aatggatatc caggaaggct tgaaaaacaa cgatgacgac ccgtccaatg
     4321 atacggatta caagatcgag tccctgacgg atgaacaggt actggaaaaa cttaaaacct
     4381 ataagagtca attgccgaaa tggatgtgga aggaaatcgt tgaagttaca gaccttcggt
     4441 tgacagaagt caccaatccg gacgaagatt gggagactct ctcctctgat gagcagaagg
     4501 aacgcaattc ggcccgggat aagaaaacag gtcaatatct caagattatg gaaaagtgga
     4561 aaaagaacaa aatgacgggc tggcgcaaag agcatgatcg cgccaatcgg ctggttgtca
     4621 caagggctgt ctgcaacgaa gtagcagaac atatccaaca cttgcgcggg catgaaggcg
     4681 ccgccgggtt agctgaaaaa cccaactggt acatggggga ggagtccaaa tattatgaat
     4741 caaaagaacg aaaacctggt tcacgcccat attttgtaaa agcaaaaggc agaaatgact
     4801 tcgaagtcgg tgcgagcatc ctttggcttc gctttgtaca cgagtctccg gatcaatgga
     4861 ggaaggcgaa agcattggaa accagggcag gggatggctt gattcctcag gcgtatttgg
     4921 ggagaagcat tgacagtggt tcttgggcgt acaaaatgga tgaccccatt gtgcggacac
     4981 ggattaaggc aactacgaat aatacgaagg gatccaataa aggcagtcgg gggggtaaag
     5041 agaatcagtg gctgcgctgg atgcatgagg cgacagtcgt tgaggtagcc gaaaccgcag
     5101 aaacaaaggg agaaaatcga gtcgtgctca cctttgaaac tgacctgccg agtgaagatc
     5161 cgagtcgatc aaccatgggc ctggtcaagc gttacttgag tgatttgacg cgtgaacaag
     5221 tggaagatag ttacaatgct gcatttgtcg gctatatgcc cgaggcaaag ctaggcgccg
     5281 acgcggctgt cggtctcgca tccgatctgg aagtgatgct ggattggaat cacatcctcc
     5341 aaagggaagt tgtttctgct gctgagatgg aaacctaccg aagcaaatac atccgcaatc
     5401 ggaaaccacc aaaacccacg attgaaataa tcgatacacc cgagcgtgta aaaggcaagg
     5461 cagatttacc catctgtgaa tttgtgaaat ggactgacag ccttgctaca aaagacaaca
     5521 acctgtggaa ggtgaggatc tggggcgacg acaagcttaa acttgtggga cttactacaa
     5581 catctgcggc tgtgcaggaa aaagctccat caaacttcca ggcagtagga ttatatgaca
     5641 aggaaacgcg ccaaaggggt gcggtatcaa actttctcaa aatcgagcgt gatgaaatcg
     5701 atgagctcat cgccatgcag attgaagatg aatttaaacg aaagcagtct gagccaaaga
     5761 ggtccagccg aaagcaactc gaaatctggc gccaacaaaa aatgcgttgg ctttgtaagg
     5821 gagaaggtac gatttacttt acaaagggtg gtaaaggctg ggaaaacgcg tcgttcattc
     5881 tatggggaac ggtttcgctg ggtgggaatc tggttcaggt ggaaggagaa gaagaaatag
     5941 agttcaaaac ggcagaaggg aaaaaaaaga tgcggatggc gaggcttgcc ggctttacaa
     6001 aatctcattg gtcctggcct cttgatgagt tgctagctca gggactcgtc catagatgct
     6061 tctgcgcact ccccggtgat aagcctactg atactccaca gggcatcgtg tatagtccgt
     6121 ttttcagtcc acggaataga gatttctcag ggcaagcgaa accgagcgca ttttaccttc
     6181 ccttcgacca acttgtaaaa cctgatgata aagattacgt tggaattagg cagagactca
     6241 aatgtaagga atgattcccc ggccgtattt ttattataaa cacgtatgag ttggtctcta
     6301 atcgatcact gcaattcata ttgaggtatg attcggtatg ggaacggaaa gccgttggct
     6361 tt
//
LOCUS       DASTJP010000821         6280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_290179, whole genome shotgun sequence.
ACCESSION   DASTJP010000821 DASTJP010000000
VERSION     DASTJP010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290179"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /gene="recO"
                     /locus_tag="VFQ23_24015"
     CDS             <1..726
                     /gene="recO"
                     /locus_tag="VFQ23_24015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET9909735.1"
                     /translation="VVLRHHDYGEADRLLTLYTRQLGKTRVLAKGARKIASRKAGHIE
                     PFTQVKLQLARGRDMLLVTQADTVDAYLPLRENLILTSQASYVLELLDRFTYVDETEN
                     PSTFRLLTDTLARLAANDDPWLVIRYYEMRLLDYLGFRPQLVQCVNCGREIQAEDQFF
                     SFRAGGVICPRCGKGLPQLKSITVETLKYLRHFQRSNYRDSSRARPNGEVQKEAEDLM
                     QGYFTYLLERELNTPGFLKKIKE"
     gene            complement(740..1861)
                     /locus_tag="VFQ23_24020"
     CDS             complement(740..1861)
                     /locus_tag="VFQ23_24020"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9909736.1"
                     /translation="MSATVLTEEKIAPGTIEWEFGTAYEMFISLHVFHEPDYYGVRAS
                     WAAGVRSRIPAPERKLLEEVFPLMSAPLAWIHSLPGPKDAISALWALKQIPPTERMIK
                     LLSLDTPHSKHGTNEDSNKYNQIRDTLLRIAETRSWKQEDLEFFMKLWGKKHSLKREK
                     LERALNWWSRPEELGEGLLSGIQSYYQAFFEEEEKRIEPVLRAGLERAQELATQLTIE
                     ELFAELSQGIQLGDEFRASKFIIAPSFWTTPLIFFDRIDRDTLFFFFGARPAEMSSIP
                     GESLPEGLVRSLKALADPTRLKILFYLSKESLSPSELAKRLHLRAPTVIHHLKELRLA
                     SLIEFGLEEDDKRYAIRRQGLESTFGNLNSFLKSDITEE"
     gene            complement(1866..2117)
                     /locus_tag="VFQ23_24025"
     CDS             complement(1866..2117)
                     /locus_tag="VFQ23_24025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909737.1"
                     /translation="MSNRPHINLIENQTIYMLKDTEYSEATETSSPASARDLFTHQTG
                     QVLVEIGNMFINIPPFNDHVQPNTGELLISIGNKLKARK"
     gene            2437..4059
                     /locus_tag="VFQ23_24030"
     CDS             2437..4059
                     /locus_tag="VFQ23_24030"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9909738.1"
                     /translation="MANETQRKTFLSYSRVNKDFALKLAKELKSEGFDVWLDQLDIPP
                     GARWDAEVEKALAESEIFMIIITPASAKSENVLDEIGYAIDSGKRILPVLLETATIPL
                     RLRRFQYVDFTSKSFHEGIESAKELLRTLINQPTLLNGEVQAETQTQAERETISPIAL
                     SVGMKGRNVSTAPSQKKPLARELVIGLIAVVVLGIAGIGYGVLSKGGSSNSPADGPTT
                     IPATEISVIATSTIALPSATLTLVQPTATVPPTNTPESAIASVPINADAAIQAAFVQS
                     AMSGDMTGRNLYIDKEKHPLASERDMLVFAMPNYKSPEPPSDYPGVYYDYPVSVWFTG
                     TQWSIVKISGMESIPWKTAFNVQILAKDSNANIHTATAENTSEYWTVIDSEKFPLASD
                     PNALVFATFNLSASAGRGKYFDKPIGVAYKDGQWIIYNRDLADMPEGAAFNVQIVNPD
                     TPGTNAFIYKVESPVDNWTIIKNPNGTPIIDANALIFAMPRGTLEGNGVKYDQNIGVW
                     LNDSMIWVIFNQKKTIPMPEGAEFNILILEPK"
     gene            complement(4223..5590)
                     /locus_tag="VFQ23_24035"
     CDS             complement(4223..5590)
                     /locus_tag="VFQ23_24035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909739.1"
                     /translation="MLVIVSDLHLGDGTTASSISPSAFHLFANRLSETAYFASFRTDG
                     TYRPLKSMDLVLMGDILDPIHSTRWLDTSPSAANFVRPWTDVSGQYYAPKLAETTQSI
                     IDVNREALEVLKRLSKGQMILLPPADSRGRPDRHSKDRIPLKLNIHYLVGNHDWYYHL
                     KGPAFDQIRRNIIDSMGLINSADPFPHEIDESPRLQEVLGAYKVFSRHGDRFDKFNFN
                     REQGRDHATLGDIFCMSVCNRYPVEVERQYGEELPKGIIDSLRRLTNIRPALATPLWI
                     SGQIKRHAGNPALEVELKKVWDDLCDEFLQLDVVREEDRAFKFDMVDALQIAIKISKR
                     TSFSTINDIVMWVRDKLWDSEHSFAEHALQEPAFLKNTAKYIVYGHTHHHEIVALDSD
                     GDLGSQENQLYINSGTWHSYFDLAVKDPSEQKFIPYQTLTYLTFYKGDEREGRHFEAW
                     SGAYA"
     gene            5848..>6280
                     /locus_tag="VFQ23_24040"
     CDS             5848..>6280
                     /locus_tag="VFQ23_24040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909740.1"
                     /translation="MASKFFSAVLIALGTIFLILSVAGIVAAWVYNEPLTLEATSRLN
                     EVDTQLSQAQTSLQSSQMELERALRIVNAAEEAMEKLVGQSTSAENIFENIQNTLDDR
                     LLPELKTTRERILAARAALENLRNALEGVSSFLSFDLNLPDE"
BASE COUNT         1672 a         1455 c         1483 g         1670 t
ORIGIN      
        1 gttgtgcttc gtcatcatga ttatggggaa gcagatcgcc tgttgacgct ctacacccgc
       61 cagcttggaa aaacacgagt acttgccaaa ggtgcgcgca agatcgcctc gcgcaaggcc
      121 gggcatattg agccattcac acaagtcaaa ttgcagcttg caaggggcag agatatgctc
      181 ctcgtcaccc aggccgatac tgtggacgcg tatctgcctt tacgagaaaa cctgatcctc
      241 accagccagg cttcctatgt gctggaatta ttggatcgtt ttacctatgt ggatgaaacg
      301 gagaacccgt ctaccttccg tttgttgacc gacactcttg cccggctcgc cgctaatgac
      361 gacccgtggc tggtcatccg ttattatgaa atgcgtttgc ttgattattt gggattccgc
      421 cctcaactcg tccaatgtgt caactgcggc cgtgagatcc aggcagaaga tcaattcttt
      481 tcattccgtg cgggaggtgt catatgccca cgctgtggaa agggattacc tcaattgaag
      541 tcaattacag tggaaaccct taagtatctc cgtcattttc aacggtcgaa ctaccgcgac
      601 tcgtctcggg cgcggccaaa tggagaggtc cagaaggaag cggaggatct catgcagggt
      661 tatttcacgt atttacttga gcgtgagttg aatacaccgg ggtttttgaa aaaaataaag
      721 gaatagatcg tggcttggtc tattcctcag tgatatcgct tttcaaaaaa gaatttagat
      781 ttccaaaggt agattcgagt ccctgcctgc gaatggcata acgtttgtcg tcttcctcga
      841 ggccaaattc gatcaggctg gcgagccgca gttccttcaa gtgatggatg accgttggag
      901 cgcggagatg gagccgcttc gcaagttcag agggggagag actttccttt gaaagataga
      961 aaaggatctt taaccgtgtg ggatcagcca gtgctttaag agatcgaacc aacccttctg
     1021 gtaacgattc gccggggatg gaagacatct ctgcgggccg tgcaccgaag aaaaagaaca
     1081 aagtgtccct gtcgattctg tcaaagaaga tcaagggcgt tgtccagaag gagggcgcga
     1141 taatgaattt gctggcgcgg aattcatcgc ccaactggat tccctgtgaa agttcagcga
     1201 ataattcttc gatggtcaac tgggttgcga gttcctgagc gcgttctaac cctgccctga
     1261 gaaccggttc aatacgtttt tcttcctctt cgaagaatgc ctgataataa gactgtatgc
     1321 cagaaaggag tccttcgcca agttcttccg gtcggctcca ccaattcagg gcgcgttcca
     1381 gcttttctcg ttttagcgag tgtttctttc cccaaagctt catgaaaaat tccaggtctt
     1441 cctgtttcca tgagcgggtc tcggctattc tcaaaagagt gtcgcgaatc tgattgtatt
     1501 tattcgagtc ctcgttcgta ccgtgtttgc tatgaggtgt atccaaactc aataatttga
     1561 tcattcgctc agtaggcgga atttgtttta aagcccataa ggcactaatg gcatctttag
     1621 ggccgggcag actatgaatc cacgccagag gggcgctcat gagcggaaag acctcctcca
     1681 gcaacttgcg ttcaggagct ggaatccgtg accggacccc ggcggcccac gaagcgcgaa
     1741 ctccgtagta atccggctcg tgaaagacat gcaggctgat aaacatttca taagccgtgc
     1801 caaattccca ctcgatcgtg ccgggtgcga tcttctcttc ggtcaagacg gttgcgctca
     1861 caggcttatt tccttgcttt cagtttgttg ccaatggaga tgagtagttc accagtattc
     1921 ggttgtacgt gatcattgaa tggcggaatg ttgatgaaca tattcccaat ctcaacgagc
     1981 acctggccgg tctggtgggt gaagaggtca cgcgccgatg cagggcttga ggtttctgta
     2041 gcttcgctgt attctgtatc tttcagcata taaatggttt gattttcgat gaggtttata
     2101 tgtggacgat ttgacatggt tagctacctt cttgatagac gcacatattg gaattaggca
     2161 attggctttg ttagactatt atctaattag ataatagtct aactcaatca tgttgtcaag
     2221 agggaatttt catgttcata actgtctgcc gggtcaggga ttgtccatcc cttctgcatt
     2281 tgcggtagat gccaaatctg ttttccccat tcaataccaa tgctttggga tgataagcaa
     2341 taggacaaat acaggtcaaa ttgcctcttg taacataatc gccctgatat ataattcctg
     2401 tgattattgg tttaagggca catacttgga gaagatatgg ctaacgaaac acagcgcaag
     2461 acattcctta gttattcacg tgtcaacaag gattttgcac tcaagttggc gaaagaattg
     2521 aaatccgaag gattcgatgt ctggcttgac cagttggata tccctccggg cgcgcgctgg
     2581 gatgctgaag ttgaaaaagc attggcagaa tccgaaattt tcatgatcat cataacccca
     2641 gcttcggcta agtcggagaa cgttcttgat gaaattggat atgccatcga ttctggcaag
     2701 cgcatcctgc ctgtattatt ggaaactgca actatacctt tgcgactccg gcgcttccag
     2761 tatgttgatt ttacgtcgaa gagctttcat gagggtattg aatctgccaa agagctgcta
     2821 agaaccttaa tcaaccaacc aaccctacta aatggagaag ttcaagcaga aactcaaact
     2881 caagcagaga gagaaacgat ttctccaata gctctgtctg ttgggatgaa aggcaggaat
     2941 gtgtcaacag caccttctca aaagaagcct cttgccaggg aacttgtgat tggtcttatc
     3001 gcagttgttg tacttggcat tgcagggatt ggatatggcg ttctctcgaa gggtggaagc
     3061 agcaattcac ccgctgatgg tcctacaaca attccagcca cagaaatatc ggtcatagca
     3121 acatctacca ttgctctacc gagcgcaaca cttacacttg ttcaaccaac cgcaacggtt
     3181 ccacccacta atacacctga gtccgccatt gcatcagtcc cgataaacgc agatgctgcc
     3241 attcaagctg cttttgtcca atccgcaatg tcgggagata tgaccggtcg taacttatat
     3301 attgataagg aaaaacaccc actcgcatcc gaacgcgata tgctggtttt tgccatgcca
     3361 aactataaaa gtccagagcc cccatctgat tatcctggag tatattatga ttatcctgtc
     3421 tccgtatggt ttacgggaac acaatggagt attgtaaaaa tcagtggtat ggagtccata
     3481 ccttggaaaa cagcgttcaa tgtccaaata cttgctaaag atagcaacgc aaatatccat
     3541 acggctacag ctgaaaatac ttcggaatat tggacggtca tcgacagtga aaaattccca
     3601 ctcgcatctg accccaatgc cttggtattt gccacgttca acttaagtgc atcggcaggc
     3661 cgtggaaaat actttgacaa gcccattggt gtagcataca aagatggtca atggattatc
     3721 tataaccggg atcttgccga catgccggaa ggcgcagcat tcaatgtgca aatcgttaat
     3781 ccagacaccc caggcacgaa cgcttttatc tacaaagtcg aatccccagt agacaattgg
     3841 acgatcatta aaaatcccaa tggcactcct attattgatg ccaatgcgct tatttttgct
     3901 atgccgcgag gcacgcttga aggcaacgga gtaaaatacg accagaacat tggtgtatgg
     3961 ctcaatgact cgatgatatg ggtcatcttc aatcagaaga aaaccatacc tatgccagag
     4021 ggtgcagagt tcaatatact gatattggag ccaaagtaag cagttaattt gaggaaaaca
     4081 tactgagctg gtcaaacagg tcagttcata ctttcaatcg ttttccccaa ttcaatacaa
     4141 acatatctgc aataaatatt gccatataag gtgctgggac attacctgtg tcagatgaca
     4201 ctcgactctt tatgtattca gtttatgcgt atgccccaga ccaggcttca aaatgccggc
     4261 cttcccgctc atcacctttg taaaaggtca aatacgtaag ggtttgatat gggataaatt
     4321 tctgctcgct gggatcctta accgccaggt caaaataaga gtgccaggtg cccgaattaa
     4381 tatataactg attttcctga ctccccagat ccccatcgga atccagcgca acgatctcgt
     4441 gatgatgtgt atgaccataa acaatgtact tcgcagtatt cttaagaaaa gccggctcct
     4501 gaagtgcatg ttcggcaaag gaatgttcgc tgtcccataa tttgtcgcgc acccacataa
     4561 caatgtcatt gattgtgctg aatgacgtgc gcttggagat cttgatcgca atctgaagcg
     4621 cgtcgaccat atcaaactta aacgccctat cctcttcgcg gacaacatcc agctggagga
     4681 attcatcgca gagatcgtcc cacactttct taagctccac ctccagggca gggtttcccg
     4741 cgtgacgttt gatctgtccg ctgatccata ggggagttgc cagcgccggg cggatatttg
     4801 tgagtcttcg caggctgtct ataattcctt ttggaagctc ctcgccatat tgcctttcca
     4861 cttcaaccgg gtatcgattg caaacggaca tacaaaagat gtcgcccaga gtggcgtgat
     4921 ctcggccttg ttcacggttg aagttgaatt tatcgaacct gtctccatgg cgactaaaaa
     4981 ctttgtaggc gcccaacacc tcttgcaaac gcggcgattc gtcgatctca tgaggaaaag
     5041 gatcagccga gttgattaat cccatcgagt cgataatatt cctgcggatc tgatcgaagg
     5101 caggtccctt gaggtgataa taccaatcgt gattaccaac caggtaatga atgttgagtt
     5161 tgagcggaat acggtcttta gagtgtctgt cgggcctgcc gcgtgagtct gcgggaggga
     5221 gcaaaatcat ctgacctttt gacaatcgct tgagaacctc tagggcttct cggtttacat
     5281 cgataatgga ctgcgtcgtc tccgcgagtt ttggcgcata atattgacca gagacatcag
     5341 tccaggggcg aacaaagttt gccgcactgg gtgaagtatc cagccagcgc gtggagtgaa
     5401 tggggtcgag aatgtcaccc atcaggacaa ggtccatgct tttgagcgga cgatacgtcc
     5461 cgtccgtgcg aaaggaggcg aaataggcag tctcgctcag gcgattggcg aacaggtgga
     5521 acgcactcgg agagatggaa ctggccgttg taccatcccc taaatgaaga tcactgacaa
     5581 taacaagcat aaattctccc tttggacatg aaatacagtt ttattatagt cctaatgctg
     5641 tacccgtatg caatacagta tcattaccag atgattctgc taatttctca ttcatcaata
     5701 aataaaatta ttgacgtata attttggaac cacattttca gggaggatgt gtggatctct
     5761 cgtttcacag catttcaagc aggcagttcc ttacaaactg cacaccactg ccaattttcg
     5821 ttgtgcttga aatttgagga ggtcgtgatg gctagtaaat ttttctcagc agttttgatc
     5881 gctctgggta cgatatttct tatcttgagt gtagcgggca ttgttgccgc ctgggtttat
     5941 aacgagcctt tgactctgga agcgacaagt agattaaatg aagtagatac ccaattgtca
     6001 caagcacaaa cgagcctgca aagctctcaa atggaattag aacgcgcgct acggattgta
     6061 aatgctgccg aagaggcaat ggaaaaactt gtagggcaat ccaccagtgc agaaaatatt
     6121 tttgaaaaca tccaaaatac tctggatgac aggttgttac ctgaactgaa aaccactcgt
     6181 gagagaatcc ttgcggcacg cgctgccctg gaaaatctgc gtaatgcgct ggaaggagta
     6241 agctctttct tatcgtttga cttaaacctg cctgatgaaa
//
LOCUS       DASTJP010000822         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_188865, whole genome shotgun sequence.
ACCESSION   DASTJP010000822 DASTJP010000000
VERSION     DASTJP010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188865"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFQ23_24045"
     CDS             complement(<1..1178)
                     /locus_tag="VFQ23_24045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909741.1"
                     /translation="MVNERRWLWGIFGFYFFLAVGYSLLMPLWEAPDEPAHYHLAWRI
                     VRVGEYASEERNYEATQPRPYYYFASLFIRALDKIDPAYSDYYFPKEFKFNIRVAKPR
                     FDWNAENYRFLLGVYVLRWVNVLFGALALWLNYKSFCLIAPDKPTLRLSALALAALTP
                     QYLHIMSSVNNDALGTVAGALLFYLAIRILNGAPVWLSLLTILLALALPLSTKLTVLP
                     VSAAVIVIIGVKWFFSLNQKRWLVIAAVLVLFVGVLIYILFPDSIQSASHEITWRLFG
                     LRKRGVDAEYLNVITNQIIQTYWGKVGWLAVGLPDWIFKLMTALGFLGAVLQVRKLIN
                     HRLTPQFTLWAGTFLIALFTILAVARNGLTTGATQGRLLFPAIGAISLLMLSGWHEVL
                     A"
     gene            complement(1172..2236)
                     /locus_tag="VFQ23_24050"
     CDS             complement(1172..2236)
                     /locus_tag="VFQ23_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909742.1"
                     /translation="MPDFNELYRKHNEQMEDFWSRRENAPRYERKIHVFGQAVSFDSN
                     LEKVLDSATLAEEMYSIADPNNEMPWQVHLTVRSMPHPSTSLALRSGSAPPPERLIDM
                     IRYTGAEDWLSIDLLEWGHCLVDMKRGEAYAVLSESLAEKPEQVCQVLINTILTNFIT
                     RHGYSMLHASALVKDEHLLLLQAPHGTGKSTTALRLLLNDYRLLSDSMVYSGERENEL
                     WMGGFPVGRIKLRTDMLPLFPALAAQAETEPVRNETKHRVELKRVDSNLAYPHMIRIR
                     RVEYCLLERWGKSESQIESLSEEDLWPEIMVNSLHYDTPELWSENLHRISLLLKHANL
                     HRLRIGTSEDEIIKTVNGLW"
     gene            complement(2330..>2818)
                     /locus_tag="VFQ23_24055"
     CDS             complement(2330..>2818)
                     /locus_tag="VFQ23_24055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909743.1"
                     /translation="ALLPGVAVTVMFPRVAKALAGGERPHRLLIQTATIILAASGALT
                     ILYFLFAEPLITIIFGNAYQPASSLLGWMGLAMIGVSLSSIWLNYYLAEKPRNFVILL
                     IAAVALEWFLLNVLPISLDNAVIAFGATGWALALSGLVMYLFKSELSLRGAAEQRRGS
                     LK"
BASE COUNT          742 a          752 c          689 g          635 t
ORIGIN      
        1 gccaaaacct cgtgccaacc actgagcatc agaagcgata tcgcaccaat ggcgggaaat
       61 aacaaccgtc cttgtgttgc tccggtggtc agtccgttgc gcgccaccgc aaggattgtg
      121 aacaatgcga tcaggaatgt cccagcccat aaagtaaatt gcggtgttaa cctgtgattg
      181 atgagttttc gaacttgaag cacggctcca agaaacccca gggcggtcat cagcttgaag
      241 atccaatccg gcaggcccac cgccagccag ccaacctttc cccagtaggt ttggatgatc
      301 tggttggtaa tgacgtttag atattcagca tcgactcccc tttttcgaag tccgaatagc
      361 ctccatgtga tttcatgaga agctgattga atcgaatctg ggaagaggat atagatgaga
      421 acgccaacaa atagtacaag cacagccgca atcaccagcc atcttttttg gttcaaacta
      481 aaaaaccatt tgactcctat gatgactata acagctgcgc tgacgggtag cacggtcaat
      541 tttgtggaga ggggcagcgc caacgcgaga aggattgtga gtagggataa ccaaacagga
      601 gctccattca aaatacgtat cgccagataa aaaagcaacg cgccggccac ggttcccaac
      661 gcgtcgttat tgaccgacga cataatatgt agatattgag gcgtaagcgc ggcaagcgct
      721 aaagcgctca gtcgtaatgt tggcttgtct ggggcgatca aacagaatga tttatagttc
      781 aaccataacg ccaaggcgcc gaacagcaca ttgacccaac gcaggacgta tacccccaac
      841 aaaaagcgat agttttccgc attccaatcg aacctgggct ttgcaacgcg aatgttgaat
      901 ttgaattcct tgggaaagta gtaatctgaa taagccgggt caattttatc cagcgcccgg
      961 atgaacaaag atgcgaagta ataataaggt ctgggttgag tcgcttcgta atttctctcc
     1021 tcggaggcat attccccaac gcgtacaatc cgccaggcga gatgataatg ggctggctca
     1081 tccggcgctt cccacagcgg catcaacagg ctatatccga ccgccaggaa aaaatagaag
     1141 ccgaagatcc cccacagcca tcgccgttcg tttaccataa gccgttgacc gtcttgatga
     1201 tctcatcttc cgacgtgccg atccgcaggc gatgtaaatt tgcatgcttc aacagcaggc
     1261 tgatcctgtg caggttttcg ctccacaatt ctggcgtgtc ataatgcaga ctattgacca
     1321 tgatctcggg ccataagtct tcttctgata acgactcaat ctggctttcg gatttgcccc
     1381 accgttccag cagacaatat tcgacgcggc ggattcggat catatgcgga taagccagat
     1441 tggagtccac tcgtttcaat tcgacccggt gcttggtctc gttgcgaaca ggttccgtct
     1501 ccgcctgtgc cgccagcgcg gggaagagtg gcagcatgtc agtgcgtagc ttgattcgcc
     1561 caacggggaa acctcccatc cataattcat tctcgcgctc accgctgtag accatactgt
     1621 ctgaaagtaa tctataatcg ttcaacaaca gccgcaatgc cgtagttgac tttccggtcc
     1681 catgaggagc ctgcaagagc aggagatgct catcttttac cagcgcgctg gcatgcaaca
     1741 tgctgtatcc gtgccgcgtg atgaaattgg tcaggatggt attgatcaac acctggcaaa
     1801 cctgttccgg tttttctgcc agcgactcag atagaactgc atacgcttcg ccgcgcttca
     1861 tatctacgag acaatgtccc cattcaagca agtcaatcga aagccaatcc tccgcgccag
     1921 tatagcggat catgtcaatc agccgctcgg gtgggggagc gctccctgag cggagcgcta
     1981 gcgaagtcga agggtgaggc atggaacgaa ccgtgagatg gacttgccag ggcatctcat
     2041 tgttggggtc agctatcgaa tacatttctt cggcaagagt cgcagagtcc aatacttttt
     2101 caagattgct atcgaagctg accgcctgcc cgaacacatg aatcttgcgt tcatatcgtg
     2161 gcgcgttctc acggcgcgac caaaaatcct ccatctgttc attgtgcttg cgatacagtt
     2221 cgttgaaatc aggcataata atcaaaacct tcgcgctgag cggagccgca ggcgtagacg
     2281 aagcggtttc gagtcaaccg cttctcccat gaaatgtttc aattggtggt tacttgagac
     2341 tgccacgtcg ctgctccgcg gctcctcgca gtgacagttc tgatttgaac aaatacatca
     2401 ctaagccact cagcgccaac gcccaccccg tcgcaccaaa tgcgatcact gcgttatcta
     2461 aacttatcgg caacacgttc aaaagaaacc attccagagc aaccgccgcg atgagaagaa
     2521 tcacaaagtt cctcggcttt tcggcaagat aataattcag ccagatcgag ctcagcgaca
     2581 caccgatcat cgcgagtccc atccagccaa gtaatgagga tgcaggttga taagcattgc
     2641 caaaaataat cgtgataagc ggctcggcaa acaagaaata aagaatcgtg agcgcgccgc
     2701 tggctgcgag aataatggtc gcagtttgga tcaacaaacg gtgaggtctc tcgcctcccg
     2761 ccagagcttt tgcaacgcgc gggaacatca cggtcactgc cacgccgggc agcagtgc
//
LOCUS       DASTJP010000823         4822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1289264, whole genome shotgun sequence.
ACCESSION   DASTJP010000823 DASTJP010000000
VERSION     DASTJP010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289264"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..2556)
                     /locus_tag="VFQ23_24060"
     CDS             complement(217..2556)
                     /locus_tag="VFQ23_24060"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909744.1"
                     /translation="MNLPNLSNTFSPVKILVVDDHPNTATTLARALSQLGPKVEVKSA
                     TNGQEAIEHVNGGATDILITDMIMPGMTGLELIEKLQNHPAGRPTFSFLVTAYDVPGL
                     KVTARRLKVKDVIVKPVHPERICQIIVQAMQEMDHAKPVRIESAVGKSFRILIADDQP
                     DNLRLLSRYLESEGYEYIEAKDGVETLEKVRKELPDLVLLDINMPRKDGFAVLEEIRA
                     DAITKHIPVIILTAARLDPTEIQSGLNMGADDYVTKPFDRRELFARIRTKLRVKEAED
                     VIRRRNRELNLLPEIGKDLSARLNMDELSNLVLRRTVETLGAEVGHILLLNSKNPYHK
                     QYRVPTSDTPETNLKLPVLNALLEQIEENRESILIDNTRDDPRWEASKDDAAHSVVIV
                     PMFGRFALIGLLVLANEQPGYFKVEHQLLLQAIASQAAIALENARLYTAMEREQQKLA
                     AILKGAADAIMMFDADTCVSMLNPAAEKLFTDHITKLGLPLARDRGYDTIIQSLEDAF
                     NSETPISREVSWPDGRVFTALFTPIAEGGCVVVLNDVTHFKRLEKVKNEFIATASHDL
                     RNPITSIRGYSQLIQHSGPLSDNQRDFVQRIQHASSHMTELVENMLDLAKMDLGAKPE
                     HEVLDMTHFLWQIADEFQPQAEAKRQLLTVAKTEQSSMVRGDGLQLRQALRNLVGNAI
                     KYTPQGGTITLSAVNQLDTVSIRIQDTGYGIPASDLPHVFDRFYRVRNNGHDEIEGNG
                     LGLAIVKSIAQQHGGDVIVASKQGEGTCFSFTLPMLAEK"
     gene            complement(2656..3456)
                     /locus_tag="VFQ23_24065"
     CDS             complement(2656..3456)
                     /locus_tag="VFQ23_24065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909745.1"
                     /translation="MKPSGHILIIDDETSLRQTLARILQRAGYEVTTAANGKDGLALV
                     TQHPFDLLYLDIRMPDISGLELLKTIHAKFPDLPVILFTAQPDLNSAVEALRRGATDY
                     LLKPLKPQAVIDRTQAILFDKQKERRRREIQRQIEALQTELMALDNDQTANQSPEQTN
                     SSDERFLRRGLLTLDMHTRRVTMNDRVINLPPTSFDYLLVLARHAPNIVDYQTLVSEA
                     QGYETDTREAQELTKWHIHHIRQAIEPDKRNPVHVINVRGSGYRLVIA"
     gene            complement(3453..>4822)
                     /locus_tag="VFQ23_24070"
     CDS             complement(3453..>4822)
                     /locus_tag="VFQ23_24070"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909746.1"
                     /translation="QIGTISVQSKMPNAFSSKDVELLKSLGVQAALAIKNTRLFESTQ
                     QRLKEVDALYKINQGLAASLNADKLIKDVIALLHQNFGYYHVQIYLLDPSSLDLILKR
                     GSGEVGEQLLRSGFRLSQGLGIVGHVAETADPFFTNNVNDVVFFSRNPLLPDTQSELA
                     VPIKLDGKVAGVLDIQHTPPHRLTDGDLQLMTAVADQLAVALQRANLYTDLQTALNHE
                     QTMRSQLVQSERLALVGRLLASVSHELNNPLQAIQNALFLIKEENLSNQASQDLDIIL
                     SETERMAALIERLRSAYRPVRLYDFNPLDLNNLIEDVHALISTHMRQKEIAFEFFPDP
                     DLPQIAGVSDQIRQVVLNLFLNAIEIMKPGGCLTVHTKSLSEQGEVFLSVKDTGPGID
                     PEILPKIFDAFITSKHTGTGLGLTITHDIIEQHHGRIQAENNADGQPGATFRVWLPID
                     RGNLE"
BASE COUNT         1164 a         1135 c         1184 g         1339 t
ORIGIN      
        1 agtgcgggtt ttctcttcat cggcttcgaa taccggggcg gtaaataacg atttaagcac
       61 aataaaggaa tttttcattg caactcctta ggatagaagg tttcatcctg cttttacttt
      121 gatcaggaat ttcataattc attaacatat tttcagcgaa atcgccgttt tggcagatgg
      181 ggttaagtga agaaaagttg gggaaagttg ttgttctcat ttttcggcta gcatcggcag
      241 agtaaagctg aaacaagtcc cttctccttg tttacttgcc acgattacat caccaccatg
      301 ctgctgtgca atggatttga caattgccag gccgagccca tttccttcga tttcatcgtg
      361 cccattattt ctcacccggt agaaacggtc gaacacatgc ggcaggtcag aagcgggaat
      421 gccatagcca gtatcctgaa tgcgaatgct gacggtatcc agctggttca ccgctgaaag
      481 tgtgattgtg ccgccttgag gggtgtactt aatcgcattt ccaacaaggt tacgaagcgc
      541 ttgtcggagt tgcagcccgt cgcctcggac catcgaactc tgttccgttt ttgcaaccgt
      601 cagcaactgt ctctttgctt cggcctgtgg ctgaaactca tctgcgatct gccagaggaa
      661 gtgggtcata tccagtactt catgttccgg ttttgcgccc agatccattt ttgcgaggtc
      721 gagcatgttc tccaccaact cggtcatgtg cgaagatgca tgttgaattc tctgcacaaa
      781 gtcacgctgg ttatcgctga ggggcccgga atgctggatg agctggctat agcctctgat
      841 ggatgtgatc gggtttctta ggtcgtgaga ggcggtggca ataaattcat tcttgacttt
      901 ttcaaggcgt ttgaagtgtg tcacatcgtt tagaaccaca acacagccac cctccgcaat
      961 cggagtgaac aatgcagtaa agacgcgccc atcgggccag gagacttccc tggaaatcgg
     1021 tgtttcagag ttgaaagcat cctcaagact ttgaatgatc gtgtcatagc cgcgatcgcg
     1081 tgcaagcggc aaccccagtt tcgttatgtg gtcagtaaac aatttttcgg cggccgggtt
     1141 cagcattgag acacaagtgt ctgcatcaaa catcatgatt gcatcggccg caccttttag
     1201 aatcgcagcc agcttttgtt gttcccgctc catcgctgtg taaagacgtg cgttttccag
     1261 cgctatcgca gcctgactgg ctatggcctg caatagcagt tgatgttcca ctttgaaata
     1321 gcctggttgt tcattggcta ataccaacag gccaatcaac gcaaaacggc caaacatcgg
     1381 aacgatgaca acagagtgag ccgcatcatc cttcgaagcc tcccagcgag ggtcgtcgcg
     1441 cgtattatcg atcaggatac tttcacgatt ttcctcgatc tgttcgagca aggcattcaa
     1501 gacaggcagt tttagatttg tttccggggt atctgaggtt gggacacgat attgtttatg
     1561 gtacggattt tttgaattaa gaagcaggat atgccctacc tctgcgccca gggtttcgac
     1621 ggttcggcga agcaccagat tcgacaactc atccatgttg agtctggcac tgaggtcctt
     1681 gccgatttcg gggagcagat taagttcgcg attccggcgg cggatcacat cttcggcttc
     1741 cttcactcgc agttttgttc gaatgcgggc aaacaactcg cgacggtcga aaggtttggt
     1801 cacataatca tcggccccca tattcaaccc ggattgaatc tctgttgggt caagccgcgc
     1861 ggcggtgaga ataatgacag gaatatgttt tgtgatagcg tccgcccgaa tttcttccag
     1921 gacggcgaag ccatctttcc ggggcatgtt tatgtcaagc aggacgagat cgggcaattc
     1981 tttgcggact ttttcaagtg tttctacgcc gtctttggct tcaatgtact catatccttc
     2041 gctttccaga tagcgtgata atagtcttag attatctggc tggtcgtctg caatgagaat
     2101 cctgaatgat ttacctactg cactttcaat tcggactggc ttggcatggt ccatttcctg
     2161 catggcttgc acaatgattt ggcagattcg ttcggggtga accggtttga cgatcacatc
     2221 tttgaccttc aagcgccgag ccgtgacctt taaccccgga acgtcataag ccgtgaccag
     2281 aaacgaaaat gtcgggcgac ccgcaggatg attttgtaat ttctcaatca gctccaaccc
     2341 ggtcatgcca ggcatgatca tatctgtaat caaaatatca gttgccccgc cattgacatg
     2401 ttcgattgcc tcctggccat tagtggcaga ctttacctct actttgggcc ccagttgtga
     2461 aagcgccctt gccagggtgg tagccgtgtt gggatgatca tccacaacga ggatttttac
     2521 cggcgaaaag gtgtttgaga gatttggtag gttcatagca atattttgca tcaacctgcc
     2581 gatcttcaac gatagggtaa attggtcaaa gttggtgaaa gttagcaaat cccccgcttc
     2641 agtacaaata cacgatcagg caattacaag tctataaccg gaccctcgga cattaataac
     2701 gtggacagga ttacgcttgt caggttcgat tgcctgacga atgtgatgga tgtgccactt
     2761 tgtcagttcc tgtgcctcac gtgtatccgt ttcatatccc tgtgcttctg acacaagagt
     2821 ttgataatcc acaatattgg gagcgtgacg cgctaaaacc aacaggtaat caaaggaagt
     2881 ggggggcagg ttgatgacgc ggtcattcat agtcacgcgg cgagtatgca tgtctaacgt
     2941 aagaagccca cgccgaagga atcgttcatc ggagctgttc gtttgttctg gcgattggtt
     3001 tgctgtttgg tcgttgtcaa gcgccattaa ctcggtttgt agagcttcaa tctgccgctg
     3061 aatctcacgt ctgcgtcgtt ccttttgctt atcgaacagg atcgcctgtg ttcgatctat
     3121 aaccgcttgc ggttttagag gcttcaataa ataatcagtg gcgccgcggc gcagggcttc
     3181 cactgcagaa ttcagatctg gttgggctgt gaatagaata acaggcaggt cgggaaattt
     3241 tgcatggatg gtctttagaa gttccaaccc acttatatcc ggcatgcgta tatccaaata
     3301 aagcaaatcg aagggatgct gtgttacaag agcaagccca tccttgccat tggcagccgt
     3361 tgtgacttca taccccgcgc gttgaagaat gcgcgccaag gtctgtcgta agctggtttc
     3421 atcgtcaatg atcagaatgt gaccgctggg tttcactcca gattccctct gtcaatgggc
     3481 aaccagactc tgaaagtagc tccgggttgc ccatccgcat tattttctgc ctggatgcgt
     3541 ccgtgatgct gttcgatgat atcgtgcgta attgtcaaac caagtccggt ccctgtgtgt
     3601 ttgctggtga tgaacgcatc aaatatcttt ggtaggattt cagggtcaat gcccgggccc
     3661 gtatctttca ccgacaggaa cacttcacct tgttcagata agctttttgt atgaacggtg
     3721 agacaaccgc caggtttcat aatttcaatt gcatttagaa atagattaag cacgacctgc
     3781 cggatctgat cggatacacc tgctatctgc ggtaggtctg ggtcaggaaa aaattcgaac
     3841 gcgatttctt tctggcgcat atgcgtggaa atcaatgcat gcacatcctc aataaggttg
     3901 ttaagatcca atgggttaaa gtcataaagc cggacagggc gataagcact acgcaatcgt
     3961 tcgatcagag cagccatgcg ttcggtctct gaaaggataa tatccaaatc ctggctagcc
     4021 tggttggaaa gattttcttc ttttattagg aataaggcat tttgtatggc ctgtaacggg
     4081 ttattcaact catgcgaaac ggaagccagc aaacgtccga caagcgcaag gcgttcgctc
     4141 tgaaccagtt gagatcgcat ggtttgttcg tgattaagcg cggtctgtag atcggtatat
     4201 agatttgccc gttggagcgc tactgccaat tgatcagcga cggctgtcat aagttgtaaa
     4261 tccccatccg taagccgatg agggggagta tgttgaatat ccagcacgcc cgcaactttt
     4321 ccgtccagct tgatagggac agccaattct gattgagtgt ctggcagaag aggattacgg
     4381 ctgaaaaata cgacatcgtt tacattattg gtgaaaaacg gatcagccgt ttcggcgacg
     4441 tgcccaacaa tccccaagcc ttgagacaga cgaaacccac tccttaacag ttgttctcca
     4501 acttccccgc ttcccctttt tagaatcagg tcaaggctcg atggatccag aagatatatt
     4561 tgtacgtgat aatagccaaa attttgatga agcagcgcta tgacatcttt gatgagttta
     4621 tcggcattca acgatgcagc taacccctga ttaatcttat aaagcgcatc tacctctttt
     4681 aatcgctgct gcgtactttc aaacaggcgc gtgtttttga tagctagtgc tgcttgaacg
     4741 cccaaagact taagcagttc cacatctttt gaagagaagg catttggcat tttgctttgc
     4801 acactgatcg taccaatttg tt
//
LOCUS       DASTJP010000824         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_183556, whole genome shotgun sequence.
ACCESSION   DASTJP010000824 DASTJP010000000
VERSION     DASTJP010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183556"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFQ23_24075"
     CDS             complement(<1..908)
                     /locus_tag="VFQ23_24075"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HET9909747.1"
                     /translation="MNSNWNLVGHDWAVDMLRKHVINGPRHAYLFTGPPGLGRRTLAL
                     SFAQALNCTTPLDAGVPCGKCRDCKQTEAMQHADLTVVQAESEGGIIKVDQIREARRT
                     LTLKPYMSRYRVALFLRFQEANDNAANALLKTLEEAPSYVVLILTADSPEQLLPTIVS
                     RCEVLRLRPLSVEVVQRELEKRGLETQGAKLIAHISGGRFGYALRLLDDAALLEKREE
                     QLEDLQRLIAASRVQKFGYADKLSKDKDGMRRTLLIWLSYWRDVMLRTAQASTPLINV
                     DRNVEIEDLAGRMNLSSTHTVVRRLE"
     gene            complement(984..1892)
                     /gene="rsgA"
                     /locus_tag="VFQ23_24080"
     CDS             complement(984..1892)
                     /gene="rsgA"
                     /locus_tag="VFQ23_24080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET9909748.1"
                     /translation="MTNDTVYSGLIIKAQSGFFTVETGQGFIVSQLRGKLKQGRATGD
                     IAAIGDRVRVTVLTDGSGAIEEVEERKQAIIRLDPRPQGEYQQVLLANADQAVFVFAC
                     ADPKPKLRMLDRFLVIAEKQRVPAVIVANKIDLVDNAKEIFGLYEPLGYRMIYTSIKT
                     GTGIDELHTTLKNKISALAGPSGVGKSSLLNEIQPGLGLAVNEISKAMKKGKHTTVTR
                     QLFPLVGGGYVADTPGWKSLALWDTEPEEMEAYFPELHELVQHCQFSDCTHTHEPNCA
                     VLAAVKEGSVHPERFESFLRLRAGQE"
     gene            complement(1898..>2345)
                     /locus_tag="VFQ23_24085"
     CDS             complement(1898..>2345)
                     /locus_tag="VFQ23_24085"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9909749.1"
                     /translation="VDGSAGELIFQPEVNGTLPVTPDRLKVIQDAMWEVVHNPLGTAN
                     FRLRGLRIPVAGKTGTAESGSGDPHAWFAGYTMASESSGLPDIAIAVVLENQGEGSDW
                     AAPVFQRIVETYYLGSPQTIYWFESNFGVTETPTPFGGIPTETPEP"
BASE COUNT          555 a          656 c          542 g          592 t
ORIGIN      
        1 ttttcgagtc ttcgtactac ggtatgtgtg gatgaaagat tcatccgccc ggccaaatct
       61 tcaatttcca cattgcggtc tacattgatc agcggagttg atgcttgtgc agttcgtagc
      121 attacatcgc gccagtagga gagccagatc aatagtgtac gccgcatacc gtccttgtct
      181 ttcgacaatt tatcggcata accaaatttc tgcacgcgcg aggcagcgat taaacgttga
      241 aggtcttcca attgttcctc gcgtttttca agcagcgccg cgtcgtcaag taatcgaagc
      301 gcgtaaccaa acctcccacc ggagatatgg gcaattagct ttgcgccctg agtctctagc
      361 cctctctttt ctaattctct ttgcaccact tcaacagaca gtggtctcag cctcaacact
      421 tcgcaacgcg agacaatcgt aggcaataac tgttcagggc tatcggcagt tagtatcagc
      481 acgacatacg agggggcttc ttccagggtt ttcaacaagg cgtttgcggc attgtcattt
      541 gcttcctgga atctcaggaa gagcgccacg cgatacctgg acatgtaagg tttgagagtc
      601 agcgttcgcc gtgcctcgcg gatctggtca accttgataa tcccgccttc cgattcagcc
      661 tgcacgaccg tcaaatctgc atgctgcatc gcttccgtct gtttacagtc acggcatttc
      721 ccgcatggaa ctccggcatc aagcggcgtc gtgcagttca gggcctgggc gaaacttaaa
      781 gctaatgtac ggcggcccag accgggaggt ccagtgaaaa gataggcatg gcgcggtccg
      841 ttaatgacat gtttcctcaa catgtccaca gcccaatcat ggcccactaa attccagtta
      901 ctattcatcc attgtcaatc gtaaatcaaa aacctgtact gagcaagtcg gaggtcggag
      961 cataggaaat cgtaaatcca aaactattcc tgtcctgctc tcaaccgcaa aaaagactcg
     1021 aagcgctcag gatgcacact tccctctttt actgcggcca gcacagcgca atttggttca
     1081 tgtgtatgcg tgcaatcact gaactggcaa tgttgcacaa gctcgtgcaa ttcaggaaaa
     1141 tacgcttcca tctcctcagg ctcggtatcc cataaagcta gcgatttcca tcccggcgta
     1201 tctgcaacat atccgccgcc caccagtgga aacaactggc gtgtcactgt cgtgtgtttg
     1261 cctttcttca tcgccttgct gatctcattc acagccagcc caagcccggg ttggatttca
     1321 ttgagcaaac tggacttacc cacgccactt ggccccgcca gcgctgaaat tttattcttc
     1381 aacgtagtat gcaattcatc aatgcccgtg cctgttttta tagaagtgta aatcatgcga
     1441 taaccgagtg gctcatacaa accaaaaatt tcctttgcat tatccactag atcaatttta
     1501 ttcgcgacga tgacggcggg cacgcgctgt ttttccgcga tgacaagaaa acgatccaac
     1561 attctcaatt ttggtttcgg atctgcgcac gcaaagacaa agactgcctg atcggcattc
     1621 gccagtagaa cctgctgata ctcgccctgc ggccttgggt caagccggat gatagcctgc
     1681 ttccgttctt caacctcctc tattgctccg cttccatccg ttaacactgt aacgcggacg
     1741 cggtccccga ttgccgcgat atcaccagtt gctctgccct gcttcaactt gccgcgcagt
     1801 tgagaaacga taaagccctg cccggtctcc actgtaaaga aaccagactg ggctttgata
     1861 attaaaccag aataaactgt atcgtttgtc aaatatttta cggctctggt gtctcggtgg
     1921 ggattccgcc aaacggagtc ggcgtttccg tcacaccgaa attggattcg aaccagtaga
     1981 tcgtttgagg cgaacctaag taatacgtct cgacgatacg ttgaaagacc ggtgcagccc
     2041 agtctgaacc ttcaccctga ttctcaagca ccactgcgat agcgatatca ggcagaccgg
     2101 agctttcact cgccatggta tacccggcaa accaggcgtg cggatcgccg cttcctgatt
     2161 ccgctgtgcc cgtcttaccg gcgactggaa tccgcaaacc gcgcaaccgg aagttggctg
     2221 tgccgagggg gttgtgtact acttcccaca tggcatcctg aatgactttc aggcggtcgg
     2281 gtgtgacagg taaggtaccg tttacttcag gttggaagat taattccccg gcactgccat
     2341 ccaca
//
LOCUS       DASTJP010000825        12231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_394984, whole genome shotgun sequence.
ACCESSION   DASTJP010000825 DASTJP010000000
VERSION     DASTJP010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394984"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFQ23_24090"
     CDS             complement(<1..232)
                     /locus_tag="VFQ23_24090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909750.1"
                     /translation="MTISAPRWDLTNIYPSLESKEFQNAVKEYKSLLDKLEKFYKNKI
                     SKASSKTSVKVLGSLLGKSVDQFNAIYELYGTI"
     gene            complement(335..1999)
                     /locus_tag="VFQ23_24095"
     CDS             complement(335..1999)
                     /locus_tag="VFQ23_24095"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9909751.1"
                     /translation="MSPNQYNFQKAIHDFQSARQKAAIQEVLARFTGKSTQLLSYDEV
                     ADKLKLRARTERGVQHIPLNAIVGSVGRDTDFTRAFLPRRAVDQQRWANVKTAMEDGA
                     GLPPIEVYKVGDVYFVIDGNHRVSIARQEGYTSIEARVIEVRTDIPLTPDIQPDDLII
                     KAEYADFLDSTKIMDLRPNVDLNVSIPGQYEKLMSQIYLQKFILVEDQKLNASLQDAV
                     EAWYDNVYISLAEIIRDRGLLRLFPNRTITDLYIWISENRTALEKELGLEIQSDVAAT
                     DLILERNARSEPGSWRKARAATRYTDHLFMDILIPLSGDAESWDALEQAILIAKREDA
                     KLLGLHVVDSKDKVNSSNALEVQSQFRQRCANANVNGNLIIESGDITRKISERAAMTD
                     LIVLKIVHPPMGGISTLRSPFRSILLNSSRPLLGVPGKATPFKRAVLAFDGSDRSKEA
                     LFVATYLAEMWKTELTVFTGVDSNKVSADTQDYVRRYLDVHEVEANYILSDGSVTDHL
                     KKTANERGADLLLMGSHGGSLMQQLLAGSALDFILRESTIPIFICG"
     gene            complement(2002..4872)
                     /locus_tag="VFQ23_24100"
     CDS             complement(2002..4872)
                     /locus_tag="VFQ23_24100"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9909752.1"
                     /translation="MNNPPSGTVTFLFTDIEGSTQLAQQYPRQMPSLLNRHHEILRSA
                     IHSHNGYIFQNEGDSLAVAFHSPMDALNTAFDAQQQLQSEAWSPAPIKVRMGIHTGAA
                     QLNDTSAPTIYTGYTTLATTSRIMSAGHGGQILISGATRELVRDVLPIDSELQDLGEK
                     RLKDLLRPERIYQLNISGFPNTFPPLKTLDAHPNNLPTQLTSFIGRENEISEVKQELA
                     AHRLVTLTGSGGTGKTRLSLQVAADLLDKFDHGVWFVELAPLTDPDLIPQAILSTIGV
                     QEQQGKTSLEVLKEYLDEKQTLILLDNCEHLISASAHIANVLLGVAPKLEILASSREA
                     LGVKGEVSYPVPSLSLPDIKNLPIIEQLSQYEAVRLFIDRALLVAPHFVVDKDNAPHI
                     AQICNRLDGIPLAIELAAARIKMLSVEQISKRLDDRFRFLTGGARTALPRQQTLRALI
                     DWSYDLLSENERLLLCRLSVFAGGWTMEAAEEVCVGQDDILPYDILDLLTQLVNKSLV
                     VVVENSQSGETRYRMLETIRQYTREKLLEAGGSESLRDQHLTHFVKLVEQAEPELYRS
                     NQIFWLNRLEDEVDNLRLALEWAVASNVRAGLKIASIPWRFWGLRGNARELGDWLERL
                     LKTETAMDALYAQALVSYAFSRFNLGDIPEALRLAEQSLQLARAISDKRMEAFSLSFL
                     GGFNVLQGKVGAGSLLLEESLTLYQSLSDEIGQINALGWLTTNSGDFERPSRITSEFL
                     HLSREVGDLASIATSLIRMARFAMWRGDFVSPVPWLEEALALARRMDDRSNEEEALST
                     YGVLSYWLGDYQRAIVFYEEAIRLGEQTGNLYQNFWAQFHMTYAILGQGDLQRARERF
                     MAGIANTYKANFLIALVYNMEGLASLLVQENQPTRSVQLFAWADTMRGQLGDQRPPVE
                     QARVERDLALIHSILNDNDFTRLSVEGRTMTLEQAIALALET"
     gene            complement(4869..7574)
                     /locus_tag="VFQ23_24105"
     CDS             complement(4869..7574)
                     /locus_tag="VFQ23_24105"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9909753.1"
                     /translation="MDRLPSGTVTFLFTDIEGSTKLWEQHPEAMKSALAKHDSILKDA
                     IESNRGHIIKTTGDGVHAVFATALDGINAAINAQRELNQISEVLIRARMGLDTGEAEL
                     RDGDYYGGTLNRAARIMSAGHGGQMLISDVTAQVAREHLADDISLQDMGECHLKGLLK
                     PEHIYQVNVPGLEHNFPPLQSLPAKTSNLPQQLTSFIGRERELSEANDRLPNARLLTL
                     VGPGGTGKTRLSLRVAEEQLPHFKDGVWFVELASISDPANIIPAISAVFELREVQGVP
                     LLTFVLDYLRAKHLLLILDNCEHLVEASAQIADQILHTSPQVKIIASSREALGINGES
                     VYRVPSLPDNDATRLFIERATKADSRFQVTEHNAPAIAQICHRLDGIPLAIELAAARV
                     KVFSAEQIAQRLDDRFKLLTGGSRTALPRQQTLRALIDWSYTSLNELEQRTLRQLAVF
                     MGGWTFDGAEAVVGESEALDGLVSLVNKSLVNVEEQESESRYRFLETIRQYAMEKLLE
                     SGEGVEARNRHLDYFMKLAEDAEQYLYGANSLGWVMRLESEYDNMRAAIEWGLDNSLA
                     AVLRMAGALPNFWFRRGYEGEGLQWIQRALDRCEELPESLGENERMKIIAKAWQAVSF
                     MAFSRGNMPEASAAAAKCATIARALGDKRLLGTVLSFEASANMMSGRIREAEPILDEA
                     LKNADESNDALAIGMAYGMGGTRRMMAGMNDERAKAMLAKGLGMLKGNENRFGYVMIS
                     FGAAMSARFNGRFAEARTQFTPLISTFRDMGDHHRSNMICSELAHMDRLEGNLERALP
                     QYRETIIEWKRLGHRAAVANQIECFAFIAKAQEQPERATILLGAAETLRELINIDMSP
                     MERIEYNREVEDLKANMNEKDFSSAWSKGRSMTMEQAIDLSLGSL"
     gene            complement(7567..10365)
                     /locus_tag="VFQ23_24110"
     CDS             complement(7567..10365)
                     /locus_tag="VFQ23_24110"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9909754.1"
                     /translation="MNSLPSGTVTFLFTDIEGSTKLAQQYPRQMPSLLNRHHEILRSA
                     IHSHNGYIFQNEGDSLAVAFHSPMEALNTASIAQKLLQSEAWSPAPIKVRMGIHTGTA
                     QLNDTSAPTIYTGYTTLATTARIMSVGHGRQVLLSEATQILIRNALPTDMELIDLGEK
                     RLKDLLRPEHLYQLNITGLQMDFPPLKTLNELRTNLPAQLTSFIGREKEVEQIKSRLG
                     KNRLVTLTGSGGIGKTRLSIQIASELLSEYPHGAWLVELASITDPEFVTREICAVLDI
                     NLPGNASPLMVLTEYLQAKKLLLVVDNCEHLIETCAQICDSLLHACPNLSVLASSREA
                     LGIDGENGYHVPSLSLPDPKSGMAAIQGTESVKLFTDRATAVLPEFELSETNASSVAQ
                     ICKRLDGIPLAIELAASRVKLLKVEQIASRLNDVFRLLTGGSRTALPRQQTLRALIDW
                     SYNLLSEPEKILFRRLAVFAGGWTMEAAESICSGKGIEREDVLDLMAHLMDKSLIVVT
                     RGGTESRYRRLETIRQYAREKLAESGESEILRNRHLEYFGGLVERFEPGLRGPDQVNL
                     MDILDIELDNLRTALEWTLDHNASAGIKLASQLKWFWHIRNHENEGVNWLVKFLIEET
                     EPLSIRSNLAVSVFDHAKALITLSYLAGNLGEMQKGMLCATEGLALCEKMSEAEGGSL
                     LAECYYLLSGGAVLSGDLHQAKALAEKSLTLYQRLGDKFGIAEVQSNRLLPIALRAGE
                     LETAHFLSEANLAIRYEIGDQDGITYELFQGGIVAMHLKNYELAQKLFLAAIDASQLG
                     RSVYILGLSLGDLGVTYLLKGEMEQARTYFLQVAKLALDKWFYIHKALTACLFALYGF
                     ETKQYRKFIKLMSFLESKNFMAMYLYSRPILETAIQKNMAIAHAEVGEEIFRQADVEG
                     KALTLDQAFDYVLEEFYG"
     gene            complement(10358..>12231)
                     /locus_tag="VFQ23_24115"
     CDS             complement(10358..>12231)
                     /locus_tag="VFQ23_24115"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9909755.1"
                     /translation="DGENAYRVPSLSLPNLKDGLSTIEQSEAVKLFMERATATLPEFK
                     LTEANASAVAQICQRLDGIALAIELAASRVKLLKVEQIASRLDDAFRLLTGGSRTALP
                     RQQTLRALIDWSYNLLSEEEKTALLRLSVFVGGWTLEAAEHVCDNSNMLDLLTHLVDK
                     SLVVVDLEQGDEPRYYFLETIRQYTREKLFDSNEMKATRQRHLTYFLAFAEMKGTETQ
                     GRDQITALKKLDDEYENIHEAMDWAIETGQMEEATQITVALAWLYWWSRGLTEEAYEK
                     INLILDHPATTKEKLFRAVALILAALYAPATVWNTGQGQAMIEEAIEISTLAGEEGKI
                     YLATAYYVWGFRMAGEDDAVAEKAFAMGLPVAKELNNKYSLANLTEFWGYLARFQKDY
                     SRALEFSKESIRLFREMGNRWGVARGLENIGLVLYSQADYQAAQKYLEESSAIYRNLA
                     DRPNLIGVLGFLGKISALTANYQQAEELFQERLFFAKDSGWAHDLGICSRDLAYLDLY
                     QGNPASALALFRESLQRFDKSDEAEISLSIAGIASAIFQLSPQNAEYAAQLWGSVQML
                     MERIGVDNLRYEKEQITKTLNAVRKHLGEASYQKFVSQGKAMSLVQALSYALEGIDE"
BASE COUNT         2929 a         3196 c         2977 g         3129 t
ORIGIN      
        1 cgatggttcc gtaaagctcg taaatggcat tgaattggtc caccgatttg ccaagcaggg
       61 aaccgagtac tttcaccgat gtttttgagc tggctttgct gatcttgttt ttgtaaaact
      121 tttcaagttt atccaaaagg cttttatatt ccttaacagc attttggaat tctttcgatt
      181 caagcgaggg atagatgttt gtcaggtccc aacgcggtgc cgagatggtc atgcagcaac
      241 tccttaggaa tgaagtgaat taagtatatc aaactccaaa aatgtgcttt gcatccacct
      301 ggccgtcgag gatcacagcc ggatccatcg attgtcagcc gcaaataaaa atcgggattg
      361 ttgattcccg taatatgaaa tccagcgcac tccccgccaa taactgttgc atcagtgatc
      421 cgccatgact gcccatcagc aacagatcgg cgccgcgttc attggccgtt ttcttcagat
      481 gatccgtgac gcttccatca ctaagaatgt agttggcttc cacctcatgc acatcaagat
      541 aacggcggac gtaatcctgc gtgtcggcgc ttaccttgtt gctatccaca ccggtgaaga
      601 cggtcaactc agttttccac atttcggcaa gatacgttgc cacaaagaga gcttccttcg
      661 agcgatcact tccatcaaaa gccagcacag cccgtttgaa tggagtggct ttccctggaa
      721 cgcccagcag ggggcgagag gagttcaaaa ggatcgaacg aaatggagag cgaagggtgg
      781 atatcccacc catcggtgga tgaacgattt tcaagacgat caagtcggtc atggccgcgc
      841 gctcgctgat cttgcgcgtg atatcaccag attcaatgat caggtttccg ttcacatttg
      901 cattcgcaca tctttgccga aattgcgatt ggacctccag tgcatttgaa ctgttcactt
      961 tatctttcga gtccaccacg tgcaaaccaa gcagttttgc atcttcacgc tttgcgatga
     1021 gtattgcttg ttccagcgcg tcccagcttt cagcatcacc actgagagga atcaggatat
     1081 ccatgaagag atgatctgta tagcgcgtgg ctgcgcgcgc ttttcgccag ctaccgggct
     1141 ccgacctggc gtttcgctcc agaatcaaat ctgtcgcggc gacgtcagac tgtatttcca
     1201 aaccaagctc cttctcaagc gcggtgcgat tctcggaaat ccatatatat aaatccgtga
     1261 tggtgcggtt gggaaacaga cgtagcaggc cgcggtcacg aatgatctca gccagagaaa
     1321 tgtaaacgtt gtcgtaccag gcctccacag cgtcctgaag cgaagcattc agtttttgat
     1381 cttccaccag gatgaacttt tgcaggtaaa tttgtgacat caatttttca tactgccctg
     1441 ggatgctgac attcaaatcc acattggggc gtaaatccat gattttggtg gaatctagaa
     1501 aatctgcata ttcggctttg atgatcaagt catctggttg aatatcaggt gtgaggggaa
     1561 tgtcggtcct gacctcgatc acgcgcgcct caatggatgt atatccctcc tgccgcgcga
     1621 tggaaacgcg gtgatttcca tcgatcacaa aataaacatc gcccactttg tacacctcaa
     1681 tcgggggcaa acctgcaccg tcttccatag cagttttcac attcgcccag cgctgctgat
     1741 cgaccgcgcg gcgcggtaga aacgcgcgcg taaaatctgt atcgcgccct acgcttccta
     1801 cgatggcatt aagtggaata tgctgaacac cgcgctctgt gcgcgcacga agcttcagct
     1861 tatcagccac ctcatcatac gatagcagct gggtggattt accggtaaag cgggcaagga
     1921 cttcctgaat ggccgctttc tgccgcgccg attggaaatc atgaatcgcc ttttggaaat
     1981 tgtattggtt gggagacatc gttaagtttc cagggcgagc gcaattgcct gctcgagtgt
     2041 cattgtacgc ccttctactg aaagacgtgt aaagtcattg tcattcaata tagaatggat
     2101 caatgccaaa tctctttcaa ccctagcctg ttcaacaggc ggacgctgat caccaagctg
     2161 accgcgcata gtatctgccc aggcgaaaag ctgcaccgag cgggttggct ggttttcctg
     2221 caccagcagg ctggccagac cctccatgtt gtacaccagc gcaatcagga aatttgcctt
     2281 gtaggtgttg gcaatgccgg ccataaaccg ctcgcgggca cgctggagat caccctgccc
     2341 caaaattgcg tacgtcatat gaaactgcgc ccaaaaattt tgataaagat tcccagtttg
     2401 ctcgcccaac cggattgctt cttcgtaaaa tacgatagca cgctggtaat cacccaacca
     2461 gtaggaaaga accccatagg ttgagagcgc ctcttcttca ttggatcgat catccattcg
     2521 acgggcaagc gcaagcgcct cctccagcca gggaaccggc gagacaaaat cccctctcca
     2581 catggcaaag cgtgccattc gtatcaggct ggtcgcgatg cttgccaggt cgcccacttc
     2641 ccgactgaga tgcaagaatt cgctcgttat acggctcggg cgctcgaaat caccactatt
     2701 ggtggttagc catcccaatg cgttgatctg cccaatttcg tccgacaaac tctgatataa
     2761 ggttaggctc tcctccagga gaagagagcc agctcccact ttcccttgca gcacattaaa
     2821 accgcccagg aacgacaaac tgaatgcctc catgcgcttg tcggagatcg ctcgagcgag
     2881 ttgcaggctt tgttctgcga ggcgaagcgc ttccggtatg tcacccagat taaaacggct
     2941 gaaagcgtaa ctgaccaacg cctgtgcata taaggcgtcc atagcggttt cggttttcaa
     3001 aagtcgctcc aaccagtcac ccaactctcg agcattgccg cgtaaacccc agaagcgcca
     3061 cggaatggag gcgattttca gaccagccct aacattgctt gccaccgccc attccagggc
     3121 caagcgcagg ttatctacct catcttccag cctgttcagc caaaagattt gattggagcg
     3181 atatagttcg ggctcggctt gttcgaccaa tttcacaaag tgtgtcaggt gctgatcgcg
     3241 aagagattcg ctgccgccgg cttcgagcaa tttttcgcgg gtgtactggc gtatggtttc
     3301 caacatgcga tagcgcgttt ctccactttg cgaattttcc acaacaacga cgagtgattt
     3361 gttgacgagt tgggtgagta gatccaatat atcgtaaggc aggatgtcat cctgccctac
     3421 gcaaacctct tccgcggctt ccatagtcca gccgcccgca aagacggaga gtcggcataa
     3481 caacaatcgc tcgttttctg agagaaggtc atagctccag tcgatcaggg cacgcaaggt
     3541 ctgttggcgc ggcaaggcgg tacgcgcgcc gccggtcaaa aagcggaagc ggtcgtcgag
     3601 tcgttttgat atctgttcga cagataacat cttgatgcgc gcggcggcaa gttcaattgc
     3661 cagaggaatg ccatccagcc gattgcagat ttgagcgata tgcggcgcgt tgtctttatc
     3721 gaccacaaaa tggggcgcga cgagcagagc gcggtcaatg aagagccgta cagcttcgta
     3781 ttgcgagagt tgctcgataa tgggtagatt tttgatatcg ggtaaggaaa gtgaagggac
     3841 gggatatgac acttcccctt tgactcccag cgcttcacgg ctcgaagcca agatttcgag
     3901 cttgggagcg acacccaaca gcacatttgc gatgtgcgcg ctggcagaga tgagatgctc
     3961 gcagttatcg agcaggatca gcgtttgttt ttcgtccaaa tattccttca atacctccag
     4021 ggaagttttt ccttgctgct cttgaacccc aatcgttgaa agaattgcct ggggtatcaa
     4081 atcgggatct gtcagcggtg caagttccac aaaccaaaca ccgtgatcga atttgtcgag
     4141 cagatcggct gcgacttgaa gagataaacg cgtcttaccc gtcccacccg aacctgtcag
     4201 cgtgaccagg cggtgcgctg ctaattcctg tttgacttcc gaaatttcat tttcgcgacc
     4261 aataaatgac gtgagttgtg tcggaagatt gttaggatga gcatcgagag ttttcagtgg
     4321 tggaaaggtg tttggaaaac cagagatatt gagttgatat atgcgttccg gacgcagtaa
     4381 gtctttgaga cgcttctcac ccagatcctg cagttcagaa tctattggaa gcacatctcg
     4441 cacaagttca cgggttgcgc ccgagataag aatttgccct ccatgcccgg cggacataat
     4501 ccgcgaagtc gtagccagcg tggtatagcc ggtgtaaatt gtcggtgcgg atgtgtcgtt
     4561 gagttgggct gcaccagtat ggattcccat acgcactttg atgggtgcag gagaccaggc
     4621 ttcgctttga agctgttgtt gggcatcgaa cgctgtattc aaggcatcca taggagagtg
     4681 gaatgccacc gcaagggaat cgccttcgtt ttgaaagata tagccattgt gggagtgaat
     4741 cgcactcctc aggatctcat ggtggcgatt caatagagaa ggcatttgcc tgggatattg
     4801 ctgcgcgagt tgggtcgagc cttcgatatc ggtgaagaga aaggtgacag tgccggaagg
     4861 gggattattc ataaggatcc caacgacaaa tcaattgcct gctccatcgt catacttcgc
     4921 cccttgctcc acgctgagct gaaatccttc tcattcatat tcgctttcaa atcctcgact
     4981 tcgcggttgt attcgatacg ttccatcggt gacatatcaa tgttgattaa ctcgcgtaat
     5041 gtctccgccg cgccgagcag gatggtggcg cgttcgggct gttcctgtgc tttggcgatg
     5101 aaggcaaaac actcgatttg atttgccacc gccgcgcgat gaccgagccg cttccattcg
     5161 ataatggtct cgcgatattg cgggagggcg cgctcgagat tcccctccag cctgtccatg
     5221 tgtgcaagtt cactgcaaat catatttgag cggtgatggt cgcccatgtc gcggaatgtc
     5281 gaaatgagcg gggtgaattg cgtccgcgcc tctgcaaagc gaccgttgaa gcgcgcgctc
     5341 attgccgcgc caaacgaaat catcacgtaa ccaaaacgat tttcattgcc ctttaacatg
     5401 ccgagaccct ttgccagcat ggctttggcc ctttcatcgt tcatgcccgc catcatgcgg
     5461 cgtgtaccgc ccatgccata cgccatgcca atggctaacg cgtcgtttga ttcgtccgca
     5521 tttttgaggg cttcatccaa aattggttcg gcttcacgaa tcctgcccga catcatattg
     5581 gcagaggctt caaacgatag caccgttccc agaagtcttt tatctcccaa cgcgcgcgcg
     5641 atggtcgcgc attttgccgc agcggcgctc gcctcgggca tattgccgcg gctgaacgcc
     5701 atgaacgaaa ctgcctgcca cgccttggcg atgattttca tcctttcatt ttcgcccagc
     5761 gactcaggca gttcttcgca gcggtcaagc gccctctgaa tccattgcag accttcccct
     5821 tcatacccgc gccggaacca aaagtttggc aatgcgcccg ccatgcgcaa cacggcggcg
     5881 agactattat ccaagcccca ctcgattgcc gcgcgcatgt tatcgtattc ggattcgagc
     5941 ctcatcaccc accccaagga attggcgcca taaaggtatt gttcggcatc ctccgccaat
     6001 ttcataaaat aatcgagatg acgattgcgc gcctccacgc cttcgcctga ttccaaaagt
     6061 ttttccatcg catattggcg gatggtctcg aggaatcgat atctggactc ggactcctgc
     6121 tcctcgacat tgaccagcga cttgttgacg agactcacca acccgtccag cgcttcggac
     6181 tcaccgacaa ctgcctctgc tccgtcgaac gtccagccac ccatgaagac cgcgagttgg
     6241 cgcagagtgc gttgctccaa ttcgttgagg gaggtataac tccaatcaat gagcgcgcgc
     6301 agagtctgct ggcgcggcaa cgccgtgcgc gagccgcctg tgagcagttt gaatctatcg
     6361 tcgaggcgtt gcgcgatttg ctccgcagaa aataccttca cgcgcgccgc ggctaattca
     6421 atcgcgaggg gaatgccgtc gagccggtgg cagatttgag cgatggctgg ggcgttatgt
     6481 tccgttacct ggaagcgtga atccgctttg gtcgcgcgtt cgatgaacag ccgcgtcgcg
     6541 tcattatccg gcagggaagg gacgcgatac acgctttcgc catttatccc caatgcctcg
     6601 cggctcgagg caatgatttt cacctgcggg ctggtgtgca aaatctggtc agcgatttgc
     6661 gcgctggctt cgacgagatg ttcgcagtta tcgaggatga gcaacagatg tttggcgcgc
     6721 aggtaatcga gcacgaacgt gaggagcggc acgccctgca cctcgcgcaa ctcgaacacc
     6781 gccgagatgg ctggaataat attcgctggg tcgctgatgg atgcaagttc aacgaaccac
     6841 acaccgtcct tgaagtgggg caattgctcc tcggcaaccc gaagcgacaa ccgcgtcttg
     6901 cccgtgccgc cgggaccgac aagagtcagc aggcgtgcat tagggagacg atcattcgct
     6961 tcactcaatt cacgctcgcg cccgataaag gatgtcagtt gttgcggtag attactcgtt
     7021 ttggccggga gcgattgcaa tggcggaaag ttatgttcga gccctggcac attgacttga
     7081 taaatgtgtt cgggtttgag caagcccttg agatgacatt cgcccatatc ctgcaacgag
     7141 atatcgtctg ccaaatgttc ccgcgccact tgcgcggtaa catccgaaat caacatctgt
     7201 cccccatgcc ccgcggacat gatccgtgcc gcgcgattaa gcgtgccacc ataataatct
     7261 ccgtcgcgca attcggcttc tccagtgtcc aatcccatgc gggcgcggat taagacttcg
     7321 gaaatctgat tcagctcacg ttgtgcatta atagcagcgt taattccatc cagcgcggtc
     7381 gcaaacaccg catggactcc atcgcctgtc gttttgatga tgtgtccgcg attcgattca
     7441 atggcatcct tcaaaatcga atcgtgtttt gccagcgcgg acttcatagc ttcggggtgt
     7501 tgctcccaaa gcttggtgct gccttcaata tcggtaaaga gaaaggtgac ggtgccggaa
     7561 ggaagtctat ccataaaatt cctccaagac ataatcaaaa gcctggtcta acgtcagggc
     7621 ttttccttcc acatctgcct gcctgaagat ttcttcgccc acttctgcgt gtgcaatcgc
     7681 catattcttt tggatggctg tttcaagaat ggggcgactg tataggtaca tagccatgaa
     7741 attcttgctt tccaaaaagc tcattagttt gatgaatttt cgatattgtt ttgtttcaaa
     7801 accatatagg gcgaatagac aggcagtgag tgccttgtga atataaaacc atttgtccaa
     7861 tgcaagtttc gcaacttgta gaaagtatgt acgcgcttgt tccatttctc ctttgagcaa
     7921 atatgtaacc cccaagtctc ccaacgaaag cccaagtata taaacacttc ttccaagttg
     7981 actggcatca atcgcagcca ggaataattt ttgagccagt tcataatttt tcaggtgcat
     8041 cgctacgata cctccctgaa ataattcgta tgtaatgcca tcctggtcac ctatttcgta
     8101 tctgatcgca agatttgcct cgctcaggaa atgagcagtt tccaactctc cggcacgtaa
     8161 cgctataggt aatagcctgt ttgattgaac ctcagcaatt ccaaatttat ccccaagtct
     8221 ttgatacaac gtcagactct tttctgccag ggcttttgcc tggtgcagat cgccgctgag
     8281 gacagctccc ccggaaagga gatagtaaca ttctgccaat aatgatcccc cctctgcctc
     8341 actcattttt tcacaaagtg ccaggccctc tgttgcacat aacatgcctt tttgcatttc
     8401 gcccaggttg ccggccaggt aggataatgt tatcaaggct tttgcatggt cgaatacact
     8461 aactgccaaa ttcgatctta tcgaaagagg ctcagtttct tcaattagaa atttcaccaa
     8521 ccagttgaca ccctcgtttt catgattgcg tatatgccag aaccatttta actgggatgc
     8581 taacttaatt cccgcagaag cattatgatc caatgtccac tccagggcgg tacgcaagtt
     8641 atcaagctca atatccagaa tatccatgag gttgacttga tctggtcctc gcaagcccgg
     8701 ttcgaagcgt tcgaccaacc cgccaaaata ttccaagtgg cgatttcgca gaatttcact
     8761 ctcaccactt tcagccaatt tttcacgcgc atactgtcga atggtttcaa gcctgcggta
     8821 acgggattcg gttccacctc ttgtgacaac aatcagggat ttgtccataa gatgtgccat
     8881 cagatcgagg acatcttcgc gctcgatccc tttgccactg caaattgatt ctgcggcttc
     8941 catcgtccat ccgcctgcaa agacagccag tctgcgaaat agaatcttct ctggttctga
     9001 gagtaggttg tagctccaat cgatcagggc acgcagggtt tgctggcgag gcagtgccgt
     9061 acggctgcca ccggtgagta agcggaagac atcatttagg cgtgaggcga tctgctccac
     9121 tttcaacaac tttacgcgtg aggctgcaag ttctattgct agcggaatgc catcgagacg
     9181 tttacagatt tgcgcgacag aagatgcgtt ggtttcactc aattcaaatt cgggcagaac
     9241 agccgttgcc cgatccgtga acaacttgac cgactcggtt ccttgaatgg cagccattcc
     9301 actttttgga tcaggcaggg agagcgaggg cacgtgatag ccattttccc catcgatgcc
     9361 caacgcttca cgactgctgg cgaggacact tagattggga caggcatgta acagtgaatc
     9421 acaaatttga gcgcaggttt caatcagatg ctcgcagtta tccactacca acagaagttt
     9481 tttcgcttgt aagtattctg tcaacaccat caatggagat gcattgcctg gtagattaat
     9541 atccaatacc gcgcagattt ctcgtgttac gaattcaggg tcggtgatcg atgcaagttc
     9601 aaccaaccat gcaccatgtg ggtattcact caacaattca gaggcaatct ggatggagag
     9661 ccgtgtcttt ccgatgccgc ctgaacctgt tagtgtgacc aagcgattct tccccagccg
     9721 actcttaatt tgttccactt ctttttcacg tccgatgaaa ctggtaagtt gagcaggcag
     9781 atttgttcgt aattcattca gcgttttcaa aggggggaaa tccatttgca accctgtgat
     9841 attcaactga tacaagtgtt caggacgaag caaatctttt aatcgtttct cgcctaaatc
     9901 aatcagttcc atatctgtgg gaagcgcgtt gcgaattagt atctgggttg cctccgaaag
     9961 caggacctgc ctaccatgcc cgaccgacat gatccgtgca gtcgtagcca atgttgtata
    10021 cccggtgtaa attgtcggtg cggatgtgtc gttgagttgg gctgtaccgg tatgtatccc
    10081 catgcgcact ttgatgggtg caggagacca agcttcgctt tggagtagtt tttgtgcaat
    10141 tgaggctgtg ttcaaagcct ccataggtga atgaaatgcc accgcgaggg aatcgccttc
    10201 gttttgaaag atatacccat tatgtgagtg aatcgcactc ctcaggatct catggtggcg
    10261 attcaataga gaaggcattt gcctgggata ttgctgcgcg agtttggtgc tgccttcaat
    10321 atccgtgaag aggaatgtga cagtgccgga ggggagacta ttcatcgatc ccctccagag
    10381 cataagaaag agcttgaacc aacgacatag cttttccttg ggatacgaac ttttgataac
    10441 ttgcttcgcc aagatgcttt cgaacagcat ttaaagtttt ggtaatttgt tctttctcat
    10501 aacgcagatt gtcaacgccg attctttcca ttagcatttg caccgacccc cacaattgcg
    10561 ctgcatattc cgcattctgc ggagacagtt gaaagatagc tgaagcgatt cctgctatgc
    10621 tgagggagat ttcagcctca tcactcttat caaagcgttg taaactttca cgaaagagag
    10681 caagagcaga ggccggattc ccttgataaa gatcgaggta agccagatcc ctactacaga
    10741 tgcctaaatc gtgtgcccat ccactatctt ttgcaaaaaa tagccgctct tgaaacaact
    10801 cttctgcctg ttgataattt gcggtcagtg cggagatttt acccaaaaat cccagaacgc
    10861 caatcaaatt aggtcgatcc gcgagattcc tgtaaatcgc ggaggattct tcaaggtatt
    10921 tttgtgctgc ctgatagtct gcctgcgaat aaagaaccaa tcctatgttt tccaaaccgc
    10981 gcgccacgcc ccatcgatta cccatttcac gaaataacct gatactctct ttggagaatt
    11041 ccagggcgcg agagtaatct ttctggaatc ttgccagata tccccaaaac tctgtcagat
    11101 tggcaaggga atatttattg ttcaattcct tggctaccgg taaccccatg gcaaatgctt
    11161 tttcagcaac tgcgtcatct tccccagcca tgcggaaccc ccagacatag tatgccgtag
    11221 ctagataaat ttttccttcc tcgcccgcaa gtgtcgaaat ttcaattgct tcctcgatca
    11281 tggcctgacc ttgtcctgta ttccaaaccg tggctggagc atacaaagca gcaagaatga
    11341 gtgctacggc ccggaatagc ttttctttgg tcgtcgcggg atgatcaaga ataagattta
    11401 tcttctcata tgcttcctca gttaatccac gactccacca ataaagccag gcaagggcaa
    11461 cagttatttg agtcgcttcc tccatctgac ctgtctcaat tgcccaatcc attgcctcgt
    11521 gaatattttc gtattcatca tcaagctttt ttagagcagt aatttgatct ctgccttgtg
    11581 tttcggtgcc tttcatttcg gcgaaagcaa ggaagtatgt caaatggcgc tggcgggtgg
    11641 ctttcatttc attcgaatcg aacagtttct cacgcgtgta ttgacgaatc gtttcaagaa
    11701 aataatagcg cggttcatcg ccttgttcga gatcaaccac gaccagggat ttatccacaa
    11761 ggtgagtgag cagatccaac atattcgagt tatcacaaac atgctctgcg gcttccagag
    11821 tccaaccgcc aacaaaaacg gataaccgca gcaacgcggt cttttcttcc tctgatagaa
    11881 ggttgtaact ccaatcgatc agggcgcgca gagtctgttg acgtggcagg gccgtgcgac
    11941 ttccacccgt gagcaaacga aaggcatcat cgagccggga ggcgatctgt tccactttca
    12001 atagtttcac gcgcgaagcg gcgagttcaa tcgccagggc aatgccatcc agccgctgac
    12061 agatttgcgc aacagcggat gcgttcgctt ctgttagttt aaattctggc aaggtggcgg
    12121 ttgcccgttc cataaataat ttcaccgctt ccgattgttc aatggtggat agtccgtcct
    12181 ttagattcgg cagcgagagc gaagggacgc gataggcatt ttcgccatca a
//
LOCUS       DASTJP010000826        10780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_541881, whole genome shotgun sequence.
ACCESSION   DASTJP010000826 DASTJP010000000
VERSION     DASTJP010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10780
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541881"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /gene="hypD"
                     /locus_tag="VFQ23_24120"
     CDS             complement(<1..1059)
                     /gene="hypD"
                     /locus_tag="VFQ23_24120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET9909756.1"
                     /translation="MKYVDEYRDAETAQHYAEAIRQITTRPWTLMEVCGGQTHAIVKF
                     GVDELLPQQITLLHGPGCPVCVTPVELIDKATEIAAQPGVIFTSFGDMLRVAGTRSDL
                     LACKAAGADVRMVYSPLDALKLAQDNPNRQVVFFAVGFETTAPANAMAIYLAHQKGIK
                     NFCMLVSHVLVPPAVEAILSSPRNRVQGFLAAGHVCTVVGYKEYEPLAQKYKTPFVVT
                     GFEPLDILQGIYMTVKQLEEGRAEVENQYVRAVHRNGNEQAQRIVRKVFTVVHRKWRG
                     IGKIPRSGLGFNPLYAEFDAEQQFGLANYHAEESSDCLSGLVLQGEKKPHECPAFGVQ
                     CTPEHPLGATMVSTEGACA"
     gene            complement(1157..4714)
                     /gene="nifJ"
                     /locus_tag="VFQ23_24125"
     CDS             complement(1157..4714)
                     /gene="nifJ"
                     /locus_tag="VFQ23_24125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HET9909757.1"
                     /translation="MNKKNFLTLDGNEAVARVAYKLNEVIAIYPITPSSAMGEWADEW
                     SAQGKPNLWNTVPTVIEMQSEGGAAGALHGALQAGALSTTFTASQGLLLMIPNMYKIA
                     GELTSTVFHVAARSLAAQALSIFGDHSDVMATRSTGFAMLCSASVQEAHDFALLAQAA
                     TLKSRVPFLHFFDGFRTSHEVAKIDLLTDEHLFSLIDAATVLEHRARALSPEHPVLRG
                     TAQNPDVYFQARETVNPFYSACPDIVQAEMDRFAALTGRQYQLFEYHGAPDATRVMVL
                     MGSGAETVHETVDYLLQQGENVGVLKVRLYRPFDKERFVAALPASVQSIAVLDRTKEP
                     GSAGEPLYIDVLTALYEAQRANIGVVGGRYGLSSKEFTPAMCKAIFDNLTQPTPKNHF
                     TVGINDDVSGTSLVYDPAFSIESERVTRALFYGLGADGTVGANKNSIKIIGESTDNYA
                     QGYFVYDSKKSGSITISHLRFGPQPIHSTYLITKANFIACHQSNLLQRFDMLQSAAPG
                     GVFLLNTPYGPQEIWEHLPRLIQEEIVAKQLKFFVIDGSGVARESGMAGRINTVMQVC
                     FFAISGVLPKDEAIEAIKHSIEKTYGRKGKEIVAMNLAAVDRTLENLHEVAVPSEATS
                     QIEMIPPIAVGAPLFARDVLGELIAGRGDCLAVSQFPVDGTFPTGTAQYEKRNLAEEI
                     PVWDPDLCIQCGKCTMVCPHAALRIKVYESEAVAGAPETFKSTTARDTEWRGLKYTIQ
                     VAPEDCTDCGLCVDICPVKSKSVPDYKALNMRPQSPLRESERANWEFFTTLPEMDRRH
                     IKSSSIRQQQVQQPLFEFSGACSGCGETPYLKLLSQLFGDRSLIANATGCSSIFGGNL
                     PTTPWAKNGDGRGPAWSNSLFEDNAEFGLGMRVALDKQNENALELIKRMSAELGDELV
                     KAILKAEQTSEAGIYEQRERISQLKSKLQQSQNPDAAYLLSLADALVKKDVWIVGGDG
                     WAYDIGFGGLDHVLASGRNVNILVLDTEVYSNTGGQMSKSTPRGAVAKFAWGGKASQK
                     KDLGLIAMSYGNVYVARVAMGAKDEHTLRAFLEAEAYDGPSLIIAYSPCREHGINLET
                     SIKNQKAIVDSGQWLLYRYHPDRAAQGENPLLLDSRAPKIPVRDYMEMENRFKMLSLS
                     KPAVADELANLAQSDVDQHWAFYEYMAKRIVHKGNEPAS"
     gene            complement(4707..4964)
                     /locus_tag="VFQ23_24130"
     CDS             complement(4707..4964)
                     /locus_tag="VFQ23_24130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET9909758.1"
                     /translation="MCLAVPGKLISMDDHPPLTRMGKVNFGGILKEVNLAYVPNVKLG
                     DYVLVHVGFALSIVDEAEANQVFEYLQKMDELAELKEASNE"
     gene            complement(4933..7284)
                     /gene="hypF"
                     /locus_tag="VFQ23_24135"
     CDS             complement(4933..7284)
                     /gene="hypF"
                     /locus_tag="VFQ23_24135"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET9909759.1"
                     /translation="MPYKPERRRLLVQGAVQGVGFRPFIYRLATEMSIQGWVSNSAQG
                     VLIEAEAPRTLLDAFLEQIEMQKPPHSSIQRIAVETIPATGIRREVAFEIRASSLEGN
                     RSTIILPDLATCPECLREIFNPDNRRYRYPFTNCTHCGPRYSIIERLPYDRPNTTMRA
                     FIMCPECRTEYENPLDRRFHAQPNACPNCGPQLSLWDATGREAAARNEALLVAADAIR
                     SGEVVALKGIGGFQLLTNACDPSAVLRLRQRKQRPDKPFAVMAPNIDQAKTICEISEE
                     QEKLLVSSEAPIVLLRRRDHNSPCAEIAPDNLNIGVMLPYTPLHHLLMAELGFPIVAT
                     SGNLSGEPICIDELESLTRLHGIADVFLIHNRPIARPVDDSVVRVMAGSECILRRARG
                     YAPLPVELSEELPVIVATGAHLKNTVALSVGQQVFVSQHIGDLDNPEAVSTFRNILKD
                     LQGLYEQQPELVACDLHPDYRSTHLAQEIAQQFGKPVIRVQHHYAHILACMAEHHLSA
                     PVLGVAWDGTGYGTDGTIWGGEFLSIGEDSFERRVHLRPFRLPGGEQAIREPRRAALG
                     LLFELYGENLPHVPQFGFSPSELNTLKAALVRKINAPLTSSTGRLFDAVGALIGLRTR
                     SSFEGQAAMDLEFVCEGIDTNKCYPIYGADVTTEEAPSGRMMDWSPMIKGILADFANG
                     VTRGEIAAAFHNSLIEMMISVATQQQIEQVVLSGGCFQNKRLLEGSIKRLRSAGFRPF
                     WSQKIPPNDGGIALGQIAAASAFRHAPILLGGQDVLSSSRQIN"
     gene            complement(7268..7642)
                     /locus_tag="VFQ23_24140"
     CDS             complement(7268..7642)
                     /locus_tag="VFQ23_24140"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET9909760.1"
                     /translation="MHEFIITETLIRGLLHQLRREHISKVLKITFRRSSTFSEEVLRQ
                     AFDILRVDTPLANAELVVEVRELHITCDCGYSSRLDRENLVGHIFMCLNCGSVREIDE
                     LHDLELVEVIAETENVLNAVQT"
     gene            complement(7647..8210)
                     /gene="hycI"
                     /locus_tag="VFQ23_24145"
     CDS             complement(7647..8210)
                     /gene="hycI"
                     /locus_tag="VFQ23_24145"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HET9909761.1"
                     /translation="MISPKSWQISLNRLMSQTGINSGGRPRIAIIGIGNELRADDAAG
                     MLVVSRLLKYGSDFDQALIRVIQAGLAPENSTSELRAFAPHLVLFVDAADMGEVAGTI
                     RWIDMEEISGISASTHSLPLSILAAYLIQEIDCQVALLGIQPASNDVGGPVSLEVLQA
                     VEEIVNELGTRFSSGEDAAMSLDTFRG"
     gene            complement(8149..8571)
                     /locus_tag="VFQ23_24150"
     CDS             complement(8149..8571)
                     /locus_tag="VFQ23_24150"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET9909762.1"
                     /translation="MKLAAMFNDVLVSFFKKPVTQLYPFQKTDPPERLRGRLYFEPGA
                     CTGCNLCVKDCPANAIELVTLDRAAKRFVLKYHMDRCIYCGQCVTGCRFKCIRMSNVD
                     WELASLKKDAFTVNYGKENDIAEILANLAQPTHEPDRD"
     gene            complement(8568..9491)
                     /locus_tag="VFQ23_24155"
     CDS             complement(8568..9491)
                     /locus_tag="VFQ23_24155"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET9909763.1"
                     /translation="MNTLLSLFNILIFPCGLTLILIGLLYDWIDRKLIARLQNRIGAR
                     WFQTFADVVKLFAKEQITPSIVNRILFFGLPVVALSGALTSALYVPWIGLPPTHSFPG
                     DLVVTLYLLSLLTMCMGIAGWNTSSRFSLVGSTRALTQLFAYEAPFLLALLGPAIAAG
                     SWQISEINSYAGSHWLLLTQPVGFVVTLIGLMGKLELPPFDAPEAETEIVAGALTDYS
                     GRGLALFNLGKSVELVVGLTLISAFYLGGLNSVPSFIIKTLALVVIMSILQSVLTRLR
                     IDQTVGLWWRYGSLLVLTQILIMILQKGWSV"
     gene            complement(9488..>10780)
                     /locus_tag="VFQ23_24160"
     CDS             complement(9488..>10780)
                     /locus_tag="VFQ23_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HET9909764.1"
                     /translation="HLFLPDDWPDDVYPLRAEFTQEQAGPVSGDDAVAVPSKGTLRGN
                     TFIVPIGPQHPALKEPGHFEFTVDGEIVTAARMRLGYAHRGIEQAAESRNWFQNLYLF
                     EHVCGICSHTHAMAYSMGVEKLAQIEVPARALAIRELVACLERIHSHLLWLGVSAHEA
                     GFDTLFMYSWRDREAVMNLLEQITGNRVNYSANLLGGVKCDIGSDQAKAIKDGVDYLE
                     GRIQHYLEIVTSDVMFLNRTRGIGSISFEETVKFGLVGPTARASGVSHDVRVEAPYGG
                     YTLFPVTAVTETNGDLEARFIVRLKELLVSCSVIRTIVANLPEGPLTTRIPRKIPAGE
                     TISRIEAPRGELFYYIKSNGGENPARVHIRTPSLCNWVSVLEKAVGAQLADIPMLISG
                     MDPCFSCNDRLVIVARPGDSQTWSWKQLREYGIEFYKR"
BASE COUNT         2558 a         2888 c         2838 g         2496 t
ORIGIN      
        1 agcacacgct ccctcggtgg ataccattgt cgcaccgagt gggtgttcgg gtgtgcattg
       61 cacgccaaag gctgggcact cgtgcggttt tttctcgcct tgcagtacta aaccgcttag
      121 gcaatcgctg ctttcttccg catggtaatt cgcgaggcca aactgctgtt cagcgtcgaa
      181 ttcagcataa agagggttga aaccaagtcc actccgaggg attttcccga tcccacgcca
      241 ttttcgatgg acaacggtaa acaccttgcg cacgattcgt tgcgcttgtt cattgccgtt
      301 gcgatgcact gctcgcacat actggttttc cacctctgcc cggccctctt cgagttgttt
      361 gaccgtcata taaatgcctt gcaaaatgtc caatggctca aaccccgtca cgacaaaagg
      421 tgtcttgtat ttttgtgcca gcggttcata ttctttgtaa ccaaccacgg tgcaaacatg
      481 tccggcggcc aggaatccct gaacgcggtt cctgggcgaa gaaaggatcg cttccactgc
      541 aggaggaacg agaacgtgtg agaccagcat gcaaaaattt ttgattccct tctgatgtgc
      601 caggtaaatc gccatggcat tagccggggc agtggtttca aagccgaccg cgaaaaagac
      661 gacctgacga ttcggattgt cctgtgcaag ttttagcgca tcaagcgggg aatagaccat
      721 gcgcacatcc gcccccgccg ctttgcaggc gagcaggtca gagcgcgtgc cggcaacccg
      781 aagcatatcg ccaaaagagg tgaagatcac gcccggctgg gccgcaatct ctgtggcttt
      841 atcgatcaac tctacgggcg tgacgcacac cggacagccg ggaccatgca gtagtgtaat
      901 ctgttgcggt aaaagctcat ccacaccaaa tttgacgatg gcatgggttt gccccccgca
      961 gacttccatc aacgtccagg gccgcgtagt gatctggcga atggcttcag cgtagtgttg
     1021 ggcagtttca gcatcgcggt attcgtcaac atatttcatt gaaagaactt ccgtttctga
     1081 gctgttcgat acagcagatg gctgcaacta ccagcctcgc tgcaacagct ggtaactcaa
     1141 tttcgttgca gacaaatcat gaagcaggct cgtttccctt atgtacaatg cgcttggcca
     1201 tatactcata gaatgcccaa tgctgatcaa catcgctctg cgccaggtta gcgagctcat
     1261 cggcaactgc gggtttactt aagctgagca tcttgaaacg attttccatt tccatatagt
     1321 ctctgaccgg aatttttggc gcacgcgaat ccaacaggag cggattttcg ccctgtgcgg
     1381 cacggtcagg atgatagcgg tagagcagcc attggcccga gtcgacaatt gctttctgat
     1441 tctttataga cgtttctaaa ttaatgccat gctctctgca gggtgagtaa gcgataatga
     1501 gcgaaggacc atcataagct tccgcttcaa ggaaggcgcg cagggtatgt tcgtcttttg
     1561 cacccatcgc aacacgcgct acatagacat ttccgtacga catggcaatc agtccgagat
     1621 cctttttctg agaagccttg ccgccccagg caaacttcgc aaccgcaccg cgcggcgtgg
     1681 atttggacat ctgtccgcca gtgtttgaat aaacttcggt gtccagaacc agtatgttga
     1741 catttcgtcc gctggcaaga acatgatcca gaccaccaaa ccctatatca taggcccagc
     1801 catctccgcc gacgatccaa acatcctttt taaccagcgc gtcagccagg ctgagcagat
     1861 aagccgcgtc gggattctgg ctctgctgca gttttgactt gagctggctg atgcgttcgc
     1921 gctgttcgta aataccggct tcgcttgtct gttccgcttt gagaatagct ttgacaagtt
     1981 cgtctcctag ttcggccgac atccgcttga tcagttcaag tgcattttca ttctgcttat
     2041 cgagtgcaac gcgcattccc aaaccaaact cggcgttatc ttcgaatagg gaattagacc
     2101 aggccggacc gcgcccatca ccattttttg cccagggtgt ggtgggaagg tttccaccga
     2161 agatcgagga gcagcccgtg gcgtttgcaa tcagggaacg gtctccgaat agttgcgaga
     2221 gcagcttgag atagggagtt tcaccgcagc ctgagcatgc accagaaaat tcaaagagcg
     2281 gctgctgaac ctgctgctgg cgaatgctac ttgatttgat gtggcggcga tccatttcgg
     2341 gtagagttgt gaagaactcc cagtttgctc gctctgattc acgcagcggc gattggggcc
     2401 gcatattcag cgctttatag tcggggaccg atttgctctt cacggggcat atatcaacgc
     2461 aaagaccgca gtccgtacaa tcttcaggag caacctggat tgtgtactta agaccgcgcc
     2521 attcagtatc gcgtgcggta gtggacttga acgtctcagg cgcgccagcc acagcttcgg
     2581 actcataaac cttgatgcgg agggcagcat gcgggcagac catcgtgcac ttgccgcact
     2641 gaatgcacag atccggatcc caaactggga tttcttctgc aaggttgcgt ttttcatact
     2701 gcgctgtgcc tgttggaaat gttccgtcca ctggaaattg gctgactgct aaacagtctc
     2761 cgcgaccggc gatcaactca ccaagcacat cacgtgcgaa cagtggcgcg ccaactgcaa
     2821 tcggcggaat catttctatc tggctggttg cctctgatgg cacggcaact tcatgcaggt
     2881 tctccaaagt gcgatctacc gcggccagat tcatggcgac aatttccttg cccttcctgc
     2941 cgtatgtctt ttcaatcgaa tgcttgatgg cctcgatagc ctcgtctttg ggtagcactc
     3001 cagagatggc gaaaaaacag acttgcatta ctgtgttgat gcggccggcc atgccacttt
     3061 cccgtgctac gccggaacca tcgatcacga aaaactttaa ctgtttagcc acgatttctt
     3121 cctgaataag cctgggcagg tgctcccata tttcctgagg accatacggc gtgttcagca
     3181 ggaacacacc tcctggagcg gcagactgca gcatgtcaaa acgttgcagc aggtttgact
     3241 ggtggcaagc tatgaaattg gctttcgtaa tcaaatatgt ggagtggatc ggctgtggac
     3301 caaagcgcaa atgcgaaatc gtaatcgagc ctgatttctt ggagtcgtag acgaaatagc
     3361 cctgagcata attatctgtg ctttcgccga tgattttgat cgagttctta ttcgcgccga
     3421 ctgtcccatc cgcgcccaaa ccataaaaca aggcccgcgt gacacgttca gattcgatgg
     3481 aaaatgccgg gtcatagaca aggctcgtcc cggaaacatc gtcgttgata cccaccgtga
     3541 agtgattttt aggagtgggc tgtgtcaggt tgtcgaagat agctttgcac atcgccggag
     3601 taaattcttt ggaggagagt ccatagcggc caccaacaac gccgatgttt gcccgttggg
     3661 cttcataaag ggctgtaagg acatcaatgt acaggggttc tccggcgctg cctggttctt
     3721 tcgtacgatc gagcacggca atggactgca cgctggccgg taacgcggca acgaaacgtt
     3781 ccttatcaaa aggccggtac aggcggactt taagtacacc cacgttttca ccctgttgca
     3841 ggaggtaatc cactgtttcg tgaacagtct ctgcccccga gcccattaag accataacac
     3901 gtgtagcatc cggagcgcca tgatattcga acaattggta ctgccgccca gtcagcgcag
     3961 cgaagcgatc catctcggcc tgaacgatat ctggacaagc cgagtagaac ggattgaccg
     4021 tttctcgcgc ctggaagtag acatccgggt tttgagccgt gccgcgcaag acagggtgct
     4081 cgggagagag cgcgcgagcg cggtgttcca acactgtggc agcgtcgatc aatgaaaaca
     4141 ggtgctcatc tgtcaacagg tcaatcttgg caacttcatg cgaagtgcgg aagccatcga
     4201 agaagtgtag gaaggggacg cgcgatttga gtgtagccgc ctgggcgagc agggcaaagt
     4261 cgtgcgcctc ttggacggaa gccgagcata gcattgcgaa gccagtggag cgcgtagcca
     4321 tcacatcgct gtggtcgcca aaaatcgata aggcttgtgc agctaacgag cgcgctgcca
     4381 catggaacac cgtcgaggtc agttccccgg cgatcttgta catgttcgga atcatcagga
     4441 gcagaccctg cgacgccgta aacgttgttg aaagtgcccc agcctgtagc gcgccgtgta
     4501 gtgctccagc cgcgccgcct tcactctgca tttcgatgac ggttggaacc gtgttccata
     4561 gattaggttt gccctgggca gaccattcat ctgcccattc ccccattgca gacgaaggag
     4621 tgatcgggta gatggcgatg acctcgttca acttataggc aacccgcgcc acagcttcgt
     4681 tgccatcgag ggtgaggaaa ttttttttat tcattggatg cttctttcaa ttcggctaat
     4741 tcgtccattt tctgcaagta ttcaaatacc tggttcgctt cggcttcatc aacaatactc
     4801 aaggcaaacc caacgtgaac caaaacgtaa tcgccaagtt tgacgttggg cacataggcc
     4861 aagttgacct ccttgagaat gccgccgaaa tttactttgc ccatgcgcgt caaaggaggg
     4921 tggtcatcca tactaattaa tttgccggga actgctaagc acatcttggc ctcctaaaag
     4981 tattggcgca tggcgaaaag cagaagctgc tgcaatttgc ccaagggcga tcccaccgtc
     5041 gttcggtggg attttctggc tccagaatgg gcgaaatccg gcacttcgta aacgtttgat
     5101 gctgccctcc agcaaacgct tattctgaaa acagccaccg ctcaatacga cctgttcaat
     5161 ttgttgttgt gtggccacgc ttatcatcat ttcgatcagc gaattatgga acgctgcagc
     5221 aatctctcca cgtgtcactc cattggcgaa gtcagcgaga atgcctttaa tcattgggct
     5281 ccaatccatc atacgtccgc tgggcgcttc ttcagtagtg acatctgccc cgtatatggg
     5341 ataacatttg ttggtatcta tgccctcaca gacaaattcc aagtccatgg ccgcttgacc
     5401 ctcgaagctg gatcgcgtac gcaatccgat gagggcgccg acagcgtcga acaaacggcc
     5461 cgtgctcgaa gtcagcggcg cattgatttt cctgacaagt gccgctttca aggtgttcaa
     5521 ctcggaaggt gaaaaaccga actgcggaac atgcggtaaa ttctcgccat acaattcgaa
     5581 aagcaggcca agcgctgccc ggcgaggttc acggatcgct tgttccccgc ccggcagccg
     5641 gaagggccgc aggtgtacgc gacgctcgaa cgaatcttcc ccgattgata aaaactcgcc
     5701 gccccatatc gtaccgtctg tgccgtagcc ggttccgtcc caggcgacgc ccagaacagg
     5761 cgcggagagg tgatgctcgg ccatacaggc caatatgtga gcgtaatgat gttgcactcg
     5821 aatcacgggt ttcccaaact gctgggctat ttcctgcgcc aggtgcgtcg agcggtagtc
     5881 gggatgcagg tcacaagcca ccagttcggg ctgttgctca tacagcccct gcaggtcttt
     5941 caaaatattt cgaaatgtcg agactgcttc ggggttgtca aggtcgccga tgtgctggct
     6001 cacaaaaacc tgttggccga cgctaagcgc aacagtattc ttaagatgcg ccccggtggc
     6061 gactatcacg ggtagttctt cggatagctc gaccggcagt ggcgcataac cgcgcgcgcg
     6121 gcgcaagata cattcgctgc ctgccatgac acgtacgaca gaatcgtcca cggggcgggc
     6181 aatgggccgg ttgtggatca ggaacacatc ggcgattccg tgtaggcggg tgagggactc
     6241 gagttcgtcg atgcaaatag gttcaccaga taaattgccg cttgttgcca caatagggaa
     6301 acctaattcg gccatcaaca aatgatggag tggcgtataa ggtagcatca cgccaatatt
     6361 gagattatcc ggcgcgatct ctgcgcacgg tgaattgtga tcgcggcgtc gcagcaatac
     6421 aatcggcgct tcggaggaga ctaatagttt ttcttgctcc tcggaaattt cgcaaatcgt
     6481 tttcgcctga tcgatgtttg gagccatcac ggcgaaaggc ttgtctgggc gttgtttgcg
     6541 ttggcgtagg cgcaacactg cactgggatc gcaagcattt gtcagtagtt ggaagccgcc
     6601 aatccccttc agcgcgacga cttcgccgct acggatagcg tcggctgcca caagtagggc
     6661 ttcgttacgc gccgcggctt cccggccggt cgcatcccac aagctgagtt gaggtccgca
     6721 gttcgggcag gcattcggct gggcgtggaa gcgtctatca agcgggtttt cgtattccgt
     6781 gcggcactcc gggcacatga tgaaggcgcg catggttgta ttcggtcggt cgtagggcag
     6841 gcgttcgatg atgctatagc gcggcccaca gtgggtgcaa ttggtaaaag ggtatcgata
     6901 gcgacggttg tccgggttga atatctcgcg caggcattct gggcaggtgg caagatcggg
     6961 caatatgatc gtcgagcgat tcccctcaag actgctggca cggatctcga aagctacctc
     7021 gcgacgtatt cccgttgccg ggattgtctc caccgcgatg cgctgtatgg aactgtgcgg
     7081 tggcttttgc atttcgatct gctccagaaa ggcatcaaga agggtgcgcg gcgcttccgc
     7141 ctcgatcagc actccctgcg cagagttact aacccagccc tggatgctca tttcggttgc
     7201 cagccgatag atgaatggtc ggaagcccac accttgcaca gctccctgca ccaggagccg
     7261 ccgtcgttca ggtttgtacg gcattgagaa cgttctccgt ttcggcgatg acttcaacta
     7321 gttccaaatc gtgcagttca tcaatttctc tgacgctgcc gcaattcagg cacatgaaaa
     7381 tatgcccaac caaattctca cggtccaagc ggctactgta accacaatcg caagtgatat
     7441 gcagctctct gacctcaaca accagttctg cattggcaag cggtgtatcc acgcgtaata
     7501 tatcgaatgc ttggcgcagc acctcttcag agaaggtgct gctgcggcgg aatgtaatct
     7561 ttaggacttt agagatatgc tcgcgccgaa gctgatgaag caagcccctg attagtgttt
     7621 cggtgatgat aaattcatgc atcggatcat cctctgaacg tgtcgagaga catcgctgcg
     7681 tcctcgcctg atgaaaatcg tgtgcctaat tcgttcacaa tttcttccac cgcctgcagc
     7741 acttcgagac tgaccgggcc accaacatca ttagatgcgg gttggatacc cagcagtgct
     7801 acctggcaat caatttcctg tatcagatag gctgccagaa tcgaaagcgg cagactatga
     7861 gtggatgcac tgattccaga aatctcttcc atgtcgatcc aacggatcgt gcctgcgacc
     7921 tcacccatat ctgccgcatc gacgaaaagg accagatgcg gtgcaaaggc gcgcagttct
     7981 gatgtgctat tttcaggggc cagccccgcc tgaataacgc ggatcaaggc ttggtcgaaa
     8041 tccgaaccgt actttaataa gcgactgact accaacatcc ctgcagcatc atccgcacgc
     8101 aactcgttcc caattcctat gatagctatg cgcggtcgcc cgccagaatt aatccctgtc
     8161 tggctcatga gtcggttgag cgagatttgc caggatttcg gcgatatcat tttcttttcc
     8221 atagttcacc gtaaacgcat ccttctttag agaagccagc tcccaatcca cgttggacat
     8281 acgaatacac ttaaatctgc aacccgtcac acattgacca caatagatgc acctgtccat
     8341 gtgatatttc agtacaaaac gcttggccgc acgatccagg gtaactaatt cgatggcatt
     8401 ggccgggcaa tctttgacac ataagttgca gccggtgcat gcgcccggct cgaaatagag
     8461 tctgcctcgc aagcgttctg gtggatccgt tttctgaaat ggataaagct gcgtgacggg
     8521 tttcttaaaa aaggatacaa gcacatcgtt gaacatggcg gctagtttca tactgaccat
     8581 cctttctgaa ggatcatgat tagtatttgg gtcagaacca gtaaagatcc ataacgccac
     8641 cagagaccga cggtctgatc gattcgcaaa cgggtgagga ctgactgcag gatggacatg
     8701 atcaccacta acgccaacgt cttaataatg aacgacggaa cactgttcag tcctcccaga
     8761 tagaaggcag agatgagagt taagcccaca accagttcca ccgatttacc caggttgaaa
     8821 agagccaggc cgcgaccgct gtaatcggtc agcgctccag ccacgatttc agtctcggct
     8881 tcaggagcat cgaagggcgg taattcaagc ttgcccatta aaccgatcag tgtgaccaca
     8941 aagccaacgg gctgggtcag aagcagccag tgactcccgg cgtaagaatt gatttcactg
     9001 atttgccaac tcccggcagc gatggcagga ccgagcaatg ctaatagaaa gggtgcttcg
     9061 taggcaaaca attgcgttag agcgcgggtg ctgcccacca atgaaaatcg gctgctggta
     9121 ttccatccag ctatacccat acacatagtt agcagactga gcaggtaaag tgtcaccacc
     9181 aggtctccgg gaaaactgtg ggttggtgga agaccaatcc atggcacata cagagccgac
     9241 gtcagcgcac cggacaacgc gacgacgggt aagccgaaga acagaattct attcacaatg
     9301 ctgggagtga tttgttcttt ggcaaacagc ttgaccacat ccgcaaaagt ttggaaccag
     9361 cgcgccccaa tgcggttctg gaggcgcgcg atcagcttgc ggtcgatcca atcatagagc
     9421 aggccgatta agatgagtgt cagcccacat gggaagatca ggatgttgaa gagagatagc
     9481 aatgtgttca tcgcttgtaa aactcaattc catactcgcg taactgtttc caggaccagg
     9541 tttgactgtc acccggccga gctacgatga cgagacgatc attgcaggag aagcacggat
     9601 ccataccaga aatcaacatg ggaatatcag ccagctgagc accaactgct ttttcaagaa
     9661 ccgagaccca attgcataag ctgggtgtgc ggatgtgaac ccgggccgga ttctccccgc
     9721 cgttactctt gatgtaataa aacagttcac ctcttggagc ttctatacgg gaaatcgttt
     9781 cgcccgcagg gatcttgcgc gggatacggg tcgtcagcgg accttcgggc aggtttgcaa
     9841 cgatggtacg tatgaccgag caggagacca gcaattcctt caaacggacg ataaagcgtg
     9901 cttcaagatc accgtttgtt tcggtgacgg ccgtaacagg aaaaagagta tatccaccat
     9961 atggagcttc cacgcgcaca tcatggctaa cacccgaagc gcgagcggtt ggaccgacaa
    10021 ggccgaactt gacagtttct tcgaaggaga tggagccaat gccgcgcgtc cgattcagga
    10081 acatcacatc tgatgtgacg atctccaggt aatgctggat gcgcccttcg agatagtcca
    10141 ctccatcttt gatcgctttt gcctggtcag aaccaatgtc acatttcacg ccgcccagta
    10201 gattggcgga gtagttaacg cgatttccgg taatttgttc gagcaaattc atcacagctt
    10261 cgcgatcacg ccacgaatac atgaacaggg tatcgaaacc agcctcatga gcggacacac
    10321 caagccacag cagatggctg tggatgcgct ccaggcacgc caccagctcg cgaatggcta
    10381 gcgcacgcgc cggaacttcg atctgcgcaa gtttttccac gcccatgctg taagccatgg
    10441 catgagtatg tgagcagatt ccgcagacat gctcgaacag gtataaattc tggaaccaat
    10501 tacgactctc agcagcctgt tcgatgccgc gatgagcata gcccagtcgc attcgtgcag
    10561 cggtgacaat ctcgccatca acggtgaatt caaaatgccc gggttctttc agcgcgggat
    10621 gttgcgggcc aatcggcacg ataaaggtgt tgccgcgaag tgtgcctttg gagggtacag
    10681 caacggcgtc atcaccggag acaggcccgg cctgttcctg agtgaattcc gctcgtagtg
    10741 ggtaaacatc atccggccag tcatccggca ggaacaggtg
//
LOCUS       DASTJP010000827         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_924714, whole genome shotgun sequence.
ACCESSION   DASTJP010000827 DASTJP010000000
VERSION     DASTJP010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924714"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..2377
                     /locus_tag="VFQ23_24165"
     CDS             98..2377
                     /locus_tag="VFQ23_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET9909765.1"
                     /translation="MTADIQSIISQMTLEEKAALCTGASGWTTTPVDRLGIPEMLVSD
                     GPHGVRRVPDIHSLGAPSLPATCFPTASCLASTWDVELLRQMGNALAEEAIALNVDVL
                     LGPGVNMKRSPLGGRNFEYFSEDPYLAGELAVALIDGIQSRGVGTSLKHFAANNQEFQ
                     RFSISAEVDERTLREIYLPAFEKAVKQAKPWTVMCAYNKLNGAYCSENHYLLTDILKN
                     EWGFEGLVVSDWGAVHDRVAALKAGLDWEMPGPQDWRVKAVIEAVRSGELDEAILDES
                     VRRILNIVFKAQETSKGGTFDVNAHHEMAHKVAAEGMVLLKNNGLLPLKDQQHIAVIG
                     RAAENAHFQGGGSSHINPTKVSMPFKELQSQAANAELTYAEGYPADDSFRQEMIDQAV
                     NLATSADVAVLYIALPSYKESEGYDRTDLDLTEQQVALIKSVAKVQPNTVVVLNNGAP
                     VAMSEWIDDVAAVLEAWMMGQAGGGAIADILFGRVNPSGKLAETYPLKLNDTPAHLNW
                     PGNSGEVRYGEGLFIGYRYYDARSLPVLFPFGHGMSYTTFTYSNPKLSATSFKDIDGL
                     TVTVDVTNTGDVMGKEIVQVYVHDQACELNRPEKELKGFVKVELQPGETKTVSIQLDF
                     RAFAFYHPEYKQWITESGDFDILIAASSTDIHHRLTATLQSTLELPCILDKESTIREW
                     TVDPRGSVVFATVFQKMKEMSRRTFGSDDDDESMGVDIMDMLMDMPLLSVLLFQQSNW
                     PTPADEIVNELLAEAHNAS"
     gene            2405..>3135
                     /locus_tag="VFQ23_24170"
     CDS             2405..>3135
                     /locus_tag="VFQ23_24170"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET9909766.1"
                     /translation="MTKTRNYLAVDLGAESGRTIVGSLADGKLTLTETHRFPNGPVHL
                     NDGLHWDVLRLWSEIKTGIGISSAKFEKKLESIGIDTWGVDFALLDKNDSLLSNPFHY
                     RDSRTDGMLEQAFQRMPREQIFTHTGIQFMQFNTLYQLLAMAMQRSPFLDVAKTLMTI
                     PDLFNFWLSGEITNEFTNATTTQCFDPRQRDWATPVLDVMNVPSHLFRPVTEPGVVIG
                     NLLPPIAEATGARMAQIIAPACHDTG"
BASE COUNT          783 a          758 c          832 g          762 t
ORIGIN      
        1 atatttccat ttggattgat tcatcgtgag tagtctttta tactgctgtt acaacagcca
       61 atcaggctcc cgaaactaat atccaaaagg aaatcagatg actgctgaca ttcaatccat
      121 catttcacaa atgactcttg aagagaaggc tgccttatgt acaggagcaa gcggatggac
      181 cacaactccg gttgaccggc ttggcatccc ggagatgctc gtctccgatg gtccgcatgg
      241 tgtgcggcgc gtaccggata tccattcatt gggcgcgcca agtctcccgg ctacctgttt
      301 cccgacggct tcctgcctgg catcgacatg ggatgtggaa ctgcttcgcc aaatgggaaa
      361 tgcgctggcg gaggaagcga tcgcattgaa tgtggatgtc ttacttggcc ccggtgtgaa
      421 catgaagcgg tctccattgg gtggccgcaa cttcgaatat ttctccgaag atccttatct
      481 ggctggcgaa cttgccgtgg ctctgatcga tggcatccag agcaggggcg tgggtacatc
      541 gctgaagcac ttcgcggcca acaatcagga atttcagcgt ttcagcatca gcgccgaagt
      601 agatgaacgg acactccgtg agatctattt gcccgccttc gaaaaagccg tcaaacaggc
      661 gaaaccctgg acggtcatgt gtgcctacaa caaactcaat ggtgcatact gttctgagaa
      721 tcactatcta ttgacagata ttcttaagaa tgaatggggt tttgaaggtt tagttgtctc
      781 agactgggga gccgtgcatg atcgtgtcgc tgcgctgaag gctgggttgg attgggaaat
      841 gccgggtccg caggattggc gcgtaaaagc ggtgatcgag gcggtccgtt ctggtgaatt
      901 ggacgaagcc attctggatg aatctgtgcg acgtatcctg aacatcgtct tcaaggcgca
      961 agagacttcc aaaggtggca cgttcgatgt gaatgctcat catgaaatgg cgcacaaggt
     1021 cgccgcggaa ggcatggtac tgctcaagaa caatggtctg ttgccgctta aagatcaaca
     1081 gcatatcgca gtgatcgggc gggcggcaga aaacgcgcac ttccagggcg gtggcagctc
     1141 acacatcaac ccgaccaaag tgtccatgcc gttcaaggaa ttgcagtcgc aggcggccaa
     1201 tgccgaatta acctatgctg aaggttaccc ggccgatgat tcgtttcgtc aggaaatgat
     1261 cgatcaggca gtgaatcttg caacctctgc agatgttgca gtcttataca ttgcgctgcc
     1321 atcctacaaa gaatccgagg gatatgatcg caccgatctc gatctcaccg aacaacaagt
     1381 ggcgctgatt aagtctgttg ccaaagttca acccaataca gttgttgtct tgaacaacgg
     1441 tgctccggtt gcgatgagcg agtggattga cgacgtggcc gctgtgctcg aagcatggat
     1501 gatggggcag gcaggcggag gtgctattgc tgatatccta ttcggcaggg tcaacccgag
     1561 cggtaagctc gctgaaacct atccgctcaa gctcaatgac acgcctgctc atctcaattg
     1621 gcccggtaac tcgggagaag tgcgttatgg agaaggtctg ttcattgggt atcgctacta
     1681 tgatgcgagg agtcttccgg tcctcttccc atttggtcat ggaatgagtt acaccacctt
     1741 cacctatagc aacccgaaac tttcagcgac aagctttaag gatatagacg gactgaccgt
     1801 tacagttgat gtgacgaaca ctggggatgt gatgggtaag gaaatcgttc aggtttatgt
     1861 tcacgaccaa gcgtgcgagt tgaaccgtcc ggaaaaggaa ttgaagggtt tcgtaaaagt
     1921 agaacttcag ccgggcgaga cgaagacggt ttccatccaa ttggattttc gcgcctttgc
     1981 gttttaccat cccgaataca aacagtggat taccgaatcg ggtgactttg acatactgat
     2041 cgcggcttcc tccacagata ttcatcacag gctgacagcc acacttcaat cgactctcga
     2101 actgccatgc attctcgaca aagaatcaac tatccgcgaa tggacagttg atccgcgtgg
     2161 cagtgttgtc tttgcgactg tgtttcaaaa aatgaaggaa atgagccgtc gaacgtttgg
     2221 cagtgacgat gacgatgaaa gcatgggtgt agacattatg gatatgttaa tggatatgcc
     2281 acttctaagt gtgctcctgt ttcagcaaag taattggcca acgcccgctg atgaaattgt
     2341 gaatgaactt ctggcagagg ctcacaacgc cagttgaatc gttcgaagat aaattcgaaa
     2401 tgtaatgacc aaaactcgta actatcttgc cgttgatctc ggcgcggaaa gcggacggac
     2461 gatcgttggc tcgctggcag acggcaaact gacactgacc gagacgcatc gtttccccaa
     2521 tggacccgtg cacctcaacg atggcttgca ttgggacgtg ttgcgtctgt ggtcagagat
     2581 caaaacaggc attggaatct catcggcaaa atttgaaaaa aaacttgaaa gcattggaat
     2641 cgatacctgg ggggtggact ttgctctact cgacaaaaac gattcgctgt tatccaatcc
     2701 gtttcactat cgcgactctc gcaccgatgg catgctggag caggcattcc agcgcatgcc
     2761 tcgggagcaa atatttacgc atactggcat tcagtttatg cagttcaata cgttgtatca
     2821 attgctggct atggccatgc agagatctcc gttcctagat gtggcaaaaa cgttaatgac
     2881 gattcccgat cttttcaact tctggttaag cggagagatc acgaacgaat tcacgaacgc
     2941 caccaccaca cagtgcttcg acccgcgcca acgcgattgg gcgacgccgg tgctggatgt
     3001 aatgaatgtc ccgtctcatc ttttccgtcc agtgaccgag cctggcgtcg tgatcgggaa
     3061 tttattgcct cccattgctg aggcaaccgg tgcgcgtatg gcacaaatta tcgctcctgc
     3121 ttgtcatgat acggg
//
LOCUS       DASTJP010000828         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1044944, whole genome shotgun sequence.
ACCESSION   DASTJP010000828 DASTJP010000000
VERSION     DASTJP010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044944"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..512)
                     /locus_tag="VFQ23_24175"
     CDS             complement(87..512)
                     /locus_tag="VFQ23_24175"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9909767.1"
                     /translation="MGTTTVKWISGKQFIGIDSTQHSVVLSTPDEGVGIKPSELLLIA
                     VASCTAVDVVEILSKKRMPLNYLEISCSGEQDQAPPWTFRKIHLRFVIGGKNLTEKAV
                     AQAIELSEEKYCSVAATVRATAQITTEFEILAEELQLVA"
     gene            complement(553..1068)
                     /gene="trxA"
                     /locus_tag="VFQ23_24180"
     CDS             complement(553..1068)
                     /gene="trxA"
                     /locus_tag="VFQ23_24180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9909768.1"
                     /translation="MEPQIFFEKLQQNSRPVVVDLWAPWCGPCKVVKPHLEKLAQEYD
                     ERVDLWEINADENPDLLHKLKVYGIPTLIGYDHGKEVVRYVGVKPKDELKSLFESLST
                     GNVPIPNGLSSLDRLIRLIFGSIVIGMGWMNDSHWLLLALGGILLFSAVYDRCPIWRA
                     ITTQFKKIAWK"
     gene            complement(1122..1322)
                     /locus_tag="VFQ23_24185"
     CDS             complement(1122..1322)
                     /locus_tag="VFQ23_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET9909769.1"
                     /translation="MKINETHIDSVIRIFASVLLLCLGFGGELAGALAIVADILGVML
                     LLTGVVGFCPLYALFRFSRSEK"
     gene            1507..1860
                     /locus_tag="VFQ23_24190"
     CDS             1507..1860
                     /locus_tag="VFQ23_24190"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9909770.1"
                     /translation="MNLFELLFGKPVSTISAVELNDRLKNGKRPVVLDVRQPDEYRSG
                     HIAGAKLIPLNRLGSRLKELPQNREIVCVCASGNRSGSATRMLVQAGLNAVNMKGGML
                     SWRRASFPVKKGDGA"
     gene            1860..2612
                     /locus_tag="VFQ23_24195"
     CDS             1860..2612
                     /locus_tag="VFQ23_24195"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9909771.1"
                     /translation="MLPLELALGFGIGLSLGLLGGGGSLLTVPALVYLVGQTPQAAMT
                     TSLAIVGTNSLMGALMHRAQGTLDWKVALIFGGTGMLASYFAVSISSQLSPEVLLITF
                     ALLMLIIGSMLFFRKNSETEADSSTKPLWLVLASGAGVGLLTGVFGVGGGFLIVPALV
                     MLVGLPIQMAVGTSLVVIAMNSFAGFLGHMGRGSIDLILTLIFASAGLAGTFAGTKLS
                     VKFSSSKLQKAFAGFVIALGFFLLYDNLPKFF"
     gene            2634..>2822
                     /locus_tag="VFQ23_24200"
     CDS             2634..>2822
                     /locus_tag="VFQ23_24200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9909772.1"
                     /translation="MFTQQFFVDGLGCASYIVGCEAKGVAAVIDPDREVRKYLDVAQS
                     RGLTITHIIETHLHADHVS"
BASE COUNT          687 a          673 c          723 g          739 t
ORIGIN      
        1 atagggagcg caagggagat tcgtttgaat tgatcttgag ttatcatttt tctgataatg
       61 cggacggatc aatcttcatt ccgaaattaa gcaacgagtt gaagctcttc tgcaaggatc
      121 tcaaattcgg tggtgatctg agcagtcgca cggacagtgg cggccaccga gcagtatttc
      181 tcctccgaaa gttcgattgc ctgagcaaca gccttctccg ttagattttt cccgccgatg
      241 acgaaacgca agtggatttt ccgaaacgtc cagggtggag cttgatcctg ctcgccgcta
      301 caactgattt ccaggtaatt cagcggcatc cgtttctttg agaggatttc tacaacatcc
      361 acagcagtac aggatgccac agcaatcagc agcaactccg aaggcttaat ccccactcct
      421 tcatcgggtg tggaaagaac aacggaatgt tgcgttgaat cgatgccgat aaattgtttt
      481 ccagaaatcc attttacagt ggtagtaccc ataggtctcc ttcggttttg ccatgcaggc
      541 taaaacatag ctttacttcc atgctatctt cttgaactgt gtggtgattg ctctccaaat
      601 cgggcatcgg tcgtataccg cgctgaagag gaggattcca cccaatgcga gaagaagcca
      661 gtgagagtca ttcatccaac ccattccaat cacaatactt ccgaagatta agcggataag
      721 acgatccaaa cttgatagtc cgtttggaat cggaacattt cccgtagaca atgattcaaa
      781 aagagatttc aattcatcct ttggctttac gccgacgtat cgcacgacct ctttaccatg
      841 atcgtagcca attagggttg ggataccata gactttcagc ttgtgcagca gatctggatt
      901 ctcatcggca ttgatttccc ataggtcaac tcgctcgtca tattcctgag ccagcttttc
      961 caaatggggt ttgacgactt tgcagggccc acaccaaggt gcccaaaggt ccaccacgac
     1021 gggacgggag ttttgttgaa gtttctcaaa aaatatttgt ggttccataa acaaatcagg
     1081 cacagtaaaa caactgtccc cgcgctcctt gtgtagaaga tttacttttc tgacctgctg
     1141 aatcgaaaca gggcatagag tgggcaaaag ccaacgacac cggttagtaa cagcatcacc
     1201 cccagtatgt cagcaacaat ggccagggcg ccagccaatt caccaccgaa gcctaaacaa
     1261 agcaacagca ccgatgcgaa aatccgaatc acgctatcaa tgtgagtttc atttattttc
     1321 atgcaagcac acgaaaccaa agtagatagt tactcttatc ttttgatgca aatcatgcga
     1381 ggaagttgca atgacgcttc cttgttttca tgcctgttac aaagatcact gcaaacgggt
     1441 gatatttgca acttttttgg aagctgagca tctaaattgt gatatcaaaa ttgggagtat
     1501 tgagaaatga atttatttga attgcttttc gggaaacctg tttcgaccat cagtgccgtg
     1561 gaattgaacg atcggctcaa gaatggcaag cgtcccgttg tgctcgatgt gcgccagccg
     1621 gatgagtatc gcagcggaca cattgcgggc gcaaagctga tccccttaaa tcgattagga
     1681 agccggttga aggagttgcc tcaaaaccgc gagatcgtct gtgtttgtgc atcagggaac
     1741 cggagtggtt ccgctacgcg gatgctcgtc caggcggggt tgaatgcagt caacatgaag
     1801 ggtggcatgc tctcctggcg gcgagcgagc ttcccggtca agaaaggtga tggggcgtga
     1861 tgctccccct cgaactggca ctgggatttg gaatcggcct ttccctcggc ttgttaggcg
     1921 gcggtggatc cctgttaacg gtgccagcac tggtctatct ggtcgggcag acgccccagg
     1981 ctgcgatgac aacatcgttg gcgattgtgg ggacgaacag tctgatgggt gccctgatgc
     2041 accgcgcaca gggaacattg gattggaagg tagcgttgat ttttggtggg acagggatgc
     2101 tggcgtctta tttcgcggtg agcatttcaa gtcaattatc ccccgaagtc ttattaataa
     2161 ctttcgcgtt gttgatgctc atcattggtt cgatgttgtt tttccgcaag aatagtgaga
     2221 cagaggcaga ctcttcaaca aagcctctct ggcttgtcct tgccagcggt gcaggcgtgg
     2281 gacttcttac tggagtgttt ggagtgggcg ggggcttcct gatcgtcccg gcgctggtga
     2341 tgttggtcgg tcttcccatc caaatggccg tggggacgtc gcttgtggta attgccatga
     2401 acagtttcgc cggtttcctg ggtcacatgg gacgcggatc cattgacttg atcttaacat
     2461 tgatctttgc ctcggccgga ctggcgggaa cgttcgccgg aacgaaactg tccgtcaaat
     2521 tctcatcgtc aaaattgcag aaggcctttg cggggttcgt catcgcgctg ggattcttcc
     2581 tgctctacga taatctgccc aaattttttt aaatgtacaa ggagagataa tctatgttca
     2641 cacaacagtt ttttgtcgac ggcctggggt gcgcctccta cattgtggga tgtgaagcaa
     2701 aaggcgtcgc cgcagtcatc gatcccgatc gcgaggtccg gaaatatctg gatgtggcgc
     2761 aaagccgcgg actgaccatc acgcatatca ttgaaacaca tcttcacgcg gatcatgttt
     2821 cg
//
LOCUS       DASTJP010000829         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_502159, whole genome shotgun sequence.
ACCESSION   DASTJP010000829 DASTJP010000000
VERSION     DASTJP010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502159"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1843)
                     /locus_tag="VFQ23_24205"
     CDS             complement(140..1843)
                     /locus_tag="VFQ23_24205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909773.1"
                     /translation="MKYINSILTVPTSDPDDARQRRLLNILLLGLFIAAMMGLIAIFT
                     YSIINPDLGRSEIQVLLGGIVVFILGLFGIYQINRRLSGRWAALLFLLLLNIIFIFTD
                     TPEELVRGRSLFLFTIPITIASLILAPQASFLFAAISSMLLAWLGTVNNLPINVFVTI
                     GFFVLALVSWLSARSLEAALKDLRAINAGLDELVAERTQALAESLERERIEAGRSRAI
                     LNSIADGVIVFDRNWNATLANPAVRSMLDFPLELIIDKNFSELIDHPKLAPKSRGLLS
                     AMMEHDTQPVGFRIEWGGKTLSVSAAQIYNRTENMGTVTVFRDFTREAEVERLKSTFV
                     AIVSHELRTPLNAVLGYAEMFKEAVYGPMNDKQVNMAERIMKNTQRLLGLINDLLDQA
                     QMEAGKLTIHMAPVRPVELLDNLHGLMDKTAADKGIALTSEIDESLPPLISGDAARLQ
                     QILVNLVNNSIKFTDQGSVHVKLFYPSSFKWGIEVSDTGQGIPENEVPYIFETFRQVE
                     GTATRVHGGFGLGLSIVKQLVNLMNGEVKVNSTLGEGTAFVITLPLVIVDVPQTNGRS
                     T"
     gene            complement(1848..>3281)
                     /locus_tag="VFQ23_24210"
     CDS             complement(1848..>3281)
                     /locus_tag="VFQ23_24210"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HET9909774.1"
                     /translation="GPGHMATTAYDTAWAARLGEVDWNLSTHALHWLAENQLPDGSWG
                     APAPMYYHDRVICTLAAMIALTYQGRREHDKEQIQKGKLALERIVGGATQGLLSDPNG
                     ATVGFEMIAPTLAAEAEKLGIIKHQGDRILGRLSKLRAKKLAYLKDHMISRNVTIAFS
                     AEMAGVDGKHMLDIENLQESNGSVGLSPSATAYFATYIKKGDEASLKYLRSVMKPDGG
                     MPNVSPFDIFEIAWTLWNLSYIPGLKNNGKLQPHLDFLSNAWQPRLGVGFASGYSVKD
                     SDDSVITYSTLLRFGIEKDIASVLAYEEKDHFRCFDLEANPSISTNIHILAALSQAGL
                     DRRNSSVQKVLHFLYKSKGQKTFWLDKWHSSPYYATAHAIIACAGLANELVEDSVQWL
                     LRSQNKNGSWGTYISTAEETAYAMQALWVWNEKVARIPKPVLANGARWLKENMDKPYP
                     PLWIGKCLYSPHLVIRSAIISALALAG"
BASE COUNT          817 a          815 c          780 g          869 t
ORIGIN      
        1 ggtgcggcac catttttcaa cctctcttgt gccactcttc catctgccac aagctctacc
       61 tcatatccaa tgccacgcag ggcttccgca aaaatgtttg ccaaatcttc gtcatcttcg
      121 atgattaagg ccagttcatt catgttgatc tcccgttggt ttgtggtacg tccactatga
      181 cgagtggaag tgtgatgaca aatgctgtcc cttcacctaa tgtactgttc accttgacct
      241 ccccgttcat caagtttacc aattgcttga cgattgacaa ccccagcccg aacccaccgt
      301 gaacccgggt ggccgttccc tccacttgac ggaacgtttc gaaaatataa gggacttcat
      361 tctctgggat gccttgacct gtatcggaca cttcgattcc ccatttgaac gaacttggat
      421 aaaacaattt tacatggact gaaccctggt ctgtaaattt tatggaattg ttcaccaggt
      481 ttaccaggat ctgttgcagg cgggcggcat ctccacttat cagaggagga agactctcgt
      541 caatttcact agtcaatgcg atgcctttgt ctgctgcggt tttgtccatc agtccatgca
      601 ggttatcaag aagctccaca ggccgtactg gagccatatg gattgtcagt tttccggctt
      661 ccatttgtgc ctgatctaaa agatcattga tcaaaccaag aagccgctgc gtatttttca
      721 tgatacgctc tgccatgttg acctgtttat cgttcatggg cccgtagact gcctctttga
      781 acatttctgc atagccaagg actgcgttca gtggtgttct taattcatga gagacaatgg
      841 caacaaaggt gctcttgaga cgttccactt ccgcctcacg ggtgaagtca cggaagacgg
      901 tcacggtacc catattttca gttcggttat agatttgcgc cgcactgacg gacaaagttt
      961 ttcctcccca ttcgatgcga aaacccacag gctgtgtatc atgctccatc atcgctgaga
     1021 gcaggccgcg gcttttcggt gcaagcttgg gatgatcgat cagttcgcta aaattcttat
     1081 caataatcag ttcgagagga aaatcaagca tactcctaac cgcgggattt gccagcgtgg
     1141 cgttccagtt tctgtcaaaa acgattacac catctgcgat ggaattcaaa atggcacggc
     1201 tccgccctgc ttctattcgt tcgcgttcga gagattccgc cagagcttgc gtacgttcgg
     1261 caactagttc atccaatccg gcattgatcg cccgcaggtc cttgagggca gcctccaaac
     1321 ttcgagcgga aagccaggaa accagggcga gaacgaaaaa accgatcgta acgaagacat
     1381 taatcggaag attattaacg gtgcccaacc aggccaggag catactgcta attgcagcaa
     1441 acagaaaact ggcttgtgga gcaagtatca aactggcaat tgttatcggg atggtaaaga
     1501 gaaataaaga ccgaccgcgg accagttctt cgggtgtgtc tgtgaagatg aaaatgatgt
     1561 ttaacagaag aagaaaaagt aaagcagccc atcttccaga caatcgacga tttatctggt
     1621 aaattccaaa aagacccaga atgaatacga cgatccctcc gaggagcacc tggatttctg
     1681 atcggccaag atccggatta ataatgctat atgtgaaaat tgcaattaaa cccatcatcg
     1741 ctgcgatgaa taaacccaat aaaagaatat tgagcagtcg tcgctggcgc gcgtcatccg
     1801 gatctgaagt tgggacggta agaattgaat ttatgtattt catagattta cccggcgagc
     1861 gccagtgcgc tgataatcgc ggatcggatg acaagatgcg gactatacaa acacttgcca
     1921 atccataacg gaggataggg cttatccata ttttccttta gccagcgtgc tccgtttgcc
     1981 aaaacaggtt tgggaattct ggcaaccttc tcgttccaga cccacagggc ctgcatggcg
     2041 tacgccgtct cttccgctgt agagatatac gtgccccacg agccgttctt attctgcgat
     2101 cttaatagcc attggacgga atcttccacg agttcattgg caagtccggc gcacgcgatt
     2161 atcgcgtggg cagtcgcata ataaggtgac gagtgccatt tatccaacca gaaggttttt
     2221 tgacccttgg acttgtatag aaagtggagc actttttgta cggaagaatt tcttcgatcc
     2281 aggccagcct ggctgagcgc tgcaaggata tggatatttg tactgatgga tggatttgcc
     2341 tccaggtcga aacagcggaa atggtctttc tcttcatatg ctaacacgct tgcgatgtct
     2401 ttttcaatgc cgaatcgtaa caaagtgctg taggtaatca cagagtcatc actgtctttg
     2461 accgaatatc ccgaggcaaa gccaacgcca agcctgggct gccaggcgtt tgaaagaaaa
     2521 tccagatgag gttgaagttt tccgttgttc ttcagcccgg gaatatagct taggttccac
     2581 agagtccagg caatctcaaa tatgtcgaac ggggacacgt tcggcattcc gccatccggc
     2641 ttcatcacgc ttcgcaggta cttcagcgat gcttcgtccc cttttttgat ataggtcgcg
     2701 aagtaggccg tagcagaggg gcttaagcca acggagccat tgctctcctg aagattctca
     2761 atatccagca tatgtttgcc atcaacgcct gccatttcag cagaaaaagc gatcgttacg
     2821 tttcggctaa tcatatgatc tttaagataa gctagttttt ttgcgcgtag tttggataaa
     2881 cgtcccagga tgcgatcccc ctgatgtttg ataattccca gtttttcagc ttctgctgcc
     2941 agcgtgggag cgatcatttc aaagccgacg gttgctccgt tgggatccga gagtaatccc
     3001 tgcgtggcgc caccgacgat tctttccaaa gcaagcttgc ctttttgaat ctgttcttta
     3061 tcatgttcac ggcggccttg ataggtgagg gcaatcatgg cggctaatgt acaaatgacg
     3121 cggtcgtgat aatacattgg cgcgggtgct ccccatgaac catctggcaa ttggttttca
     3181 gcaagccagt gtagtgcgtg cgtgcttaga ttccagtcca cttcaccaag gcgggctgcc
     3241 caggctgtgt catacgccgt cgttgccata tggccgggac c
//
LOCUS       DASTJP010000830        10730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1078519, whole genome shotgun sequence.
ACCESSION   DASTJP010000830 DASTJP010000000
VERSION     DASTJP010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078519"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="VFQ23_24215"
     CDS             <1..1570
                     /locus_tag="VFQ23_24215"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9909775.1"
                     /translation="GFLIINTITALMTQQTRQVGIMKATGGGTSQIFGMYIVLIVCFG
                     LAALLIAVPLANLSAQAIGGGMAGWLNFHPSPYKGYAATLLQQFFVAVIIPLIAAIFP
                     IYNSVRVTVREALSDYGLGGNAKVKADSVNKNSVLIPRPIRISLRNTFRRKTRLALTL
                     FTLVLGGAVFIAVYNLWASFDKVIEDIQGYFLADINISFNRAYRFDEVAGIAQNVPGV
                     EGVEGWLEYAGTLIADETAAGTQILFVAPPSDSTLIDPIITKGRWLKTGDENAIVIGN
                     HLLQMFPDLQIGDELIIETNGKKSPWKIVGFYSITGNVSPPLLYVNYEYISRLINQPG
                     QVYSLRVITASRDGDFQRRINDQLQAAYESHGVQIGSSQLGSEFIESQKSQTDILVYF
                     MLVMASLIAIVGGLGLMGTMSINVLERTREIGVMRAIGASNGDIQSIVIIEGMVIGLL
                     GWAIAILFAVPITSVLTTGVGLAILTAPMPAVYDVNGIIAWLIFTLVLATIASTLPAR
                     RASRLTVRDTLAYE"
     gene            1701..3017
                     /locus_tag="VFQ23_24220"
     CDS             1701..3017
                     /locus_tag="VFQ23_24220"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9909776.1"
                     /translation="MGRIDKTVFISYRNTNQPWALAIYQHLIHQGFNVFFDYNILKSG
                     DFEQVITENIRGRTHFVVLLTPSALERCNEPGDQMRREIELALDEKRNIVPLFFEGFS
                     FSSPSISKYLTDRLDLLKKHKEITLSAEYFDAAMARLCNDFLNVPFAAILHPLSDTVE
                     NAVKMQQAAARKAKKIQHNELAAQEWFEQGKHYDDSNDFDECINSYTKAIGLKSDYVE
                     AYLRRGVACHEKGDMDGALKDTNEAIRLNPDFAEAHFIRGRARYYKSDVEGAIQDYNK
                     AIRLNPEYALAYYNRGIARSDKGDLAGAIKDYGEAIRFSPDYALAYYNRGIARGDKGD
                     LAGAIKDYSETIRLSPEYADAYNNRAILRSDKGDLAGALEDYNEAIRLNPSDADAYIN
                     RGILWEEYKNNYDAAIADFQRYIDLGGERKEVAEWVTDLKKKSNSE"
     gene            3130..6021
                     /locus_tag="VFQ23_24225"
     CDS             3130..6021
                     /locus_tag="VFQ23_24225"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HET9909777.1"
                     /translation="MDLQSQLSQIIHPSRVLTSHLHRIAYANDASYFRLLPQAVVQPN
                     SISEIQSLFKFTQQNNIPMTFRAAGTSLSGQAVTDGVLVDISKHWGNYRIEENGELIR
                     FQPGIVGGFINNVLKGYGRRIGPDPASIDACMMGGILANNSSGMCCGVIENSYHTIHS
                     MILVLPNGFVLNTADSNANQIFEDQQPHLAKGLLELKKRIQASSFSGKGGTNGEVESL
                     AERIHRRYQMKNTNGYLLNALLEFDTPLDILTHIMIGSEGTLGFIAEGVLHTLPDMPR
                     RYTGQLYFKNLQDAASAILPIKESGARAVEIMDRAALRSVENNAGVPSILRELPEGAT
                     AILVEYQAQDAESLFRSTKEAEQVIRKLKLIHDPEFTGDPAQQAALWKTRKGIIPSIG
                     GMRAPGTVCINEDVVFPIHRLAEAVTDLQHLFADFGYTDGAVFGHAKDGNLHFLINQA
                     FNTNAEVTHFDRFVHAMVQLVSGKYDGALKAEHGTGRNMAPFVETEWGSEAYAIMRDL
                     KSLFDPDYMLNPGVMINHDPQAHVQHLKTMSLVSPEVDKCIECGFCESKCPSRQLTLT
                     PRQRIVVQREASRLKALGSDSSTLESISKDFSYAGMDTCAVDGLCATACPVSINTGDL
                     TRRLRSESSTPGGNQTAKWLADHFSLTEFAVGSAAQLGHIAESMLGVKGINSVIQTAE
                     KISKTTLPKWNSHIPSPSPKRREVRGEVNKDFIYFPSCISRQLGKPKGGSHLSLPETL
                     ITLANRSGISLWIPPDANGTCCGMPFHSKGFESAYHASLHKTFANLWEWSEQGKYPIV
                     VDTTSCTHTLRTCQEDLDTEDKEIWSNLAFLDGLDFLNDFVLPKLAVHPIDEDIILHP
                     NCSARKLGLDSKMLAIAKQCARSATVPLHLGCCAFAGDRGLLFPELTRSATEKESAEV
                     NTREYGGYYSSNIPCEIGMSEATGKDYTSIAYLVERASRKQDEKESVRY"
     gene            5996..6661
                     /gene="nth"
                     /locus_tag="VFQ23_24230"
     CDS             5996..6661
                     /gene="nth"
                     /locus_tag="VFQ23_24230"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9909778.1"
                     /translation="MKKKAFDIDLALEGISEAVKPWPKAAMFELAEEGFDSPFEQLVA
                     CIISIRTYDEVSLPTARALFALARTPAQISKLSPEEIDGLIHACTFHERKAAQIHAIA
                     KRVVVEYEGNLPCEAELLLSFSGVGPKCANLALGIACGQPAISVDIHVHRVTNRWGYV
                     KASTPEKTMLALEAKLPREHWININRLLVPFGKHICTGTMPKCSTCPVLDMCAQVGVK
                     THR"
     gene            6671..7630
                     /locus_tag="VFQ23_24235"
     CDS             6671..7630
                     /locus_tag="VFQ23_24235"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9909779.1"
                     /translation="MNIFITGASGFIGGAITKALAASHNVYGMSRSNQSDDHIRSLGA
                     TPVRCELGAVRPEHLADCEVVIHCAAFVKQCGTREEFWKTNVDGTAQLLEVARLAGVR
                     RFIHVGTEAALFHGQHMRDMDETYPYPDSTPFLYPETKAAAEKLVLSANAPGFETISL
                     RPRFVWGPGDQTILPVVKKMVNKGMYMWIDQGRVRTSTTHIDNFVHAVTLALTRGRGG
                     EAYFITDDSTSTVREFLTALLKTQAITMPNTSIPSWLARALGWSIEGIWKLLRLKSEP
                     PLTHFAAAMASSECTIRIDKARAELGYAPVMTIDQGLAELANP"
     gene            7720..>10730
                     /locus_tag="VFQ23_24240"
     CDS             7720..>10730
                     /locus_tag="VFQ23_24240"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9909780.1"
                     /translation="MAKVFVSYSRKDIEFAKRLTAELQKSDLDFWIDWEGIPPTVDWW
                     REIEKGIEESDVFIFLISPDSAKSKICGQEIDTAVKNGKRLIPLVVRDIKGDETPSQL
                     SHLNWIFFREGDDFDAGLQKLVTGIHTDYEWVKEHRWLQVRALDWERENKDSGSLLRG
                     KDLQDAEFQLATNTSKEPHPTDLQREYVFTSRTAADRQRRVTMGFSIAGIIALATLAI
                     WGWGQAGQATLNADEANKNAAIANAASTLAFENAIRADHEAAIAQAASTFAVSKEQEA
                     ERQAQISLARQWAAEANFLLANQNGNTETAALLSLRALQLHDVYLPSADAALVASLNK
                     LYTVKIFDEHESKVSAVAYSPNGDRISIGYGDGTVGLYDLPVGKEIRHFNVELRDYEG
                     DPYETGIHALSFSADGNSLLIADSGGRVVVWNLVTFTIEVDFYVQENLPLWSAKFSPN
                     GQFILISTGFPHSGPGNIYLYNLQNGEKANISFAQLQQRYVTDVEFSSDGSQVLSTSW
                     DKSAILWDINWESLEATPIQTFELSSPIRDGAISANGELILLGTDNNSAELYTSGGVL
                     KQTYVGHTSYVYSVNFSPDGRHILTGSWDKTARLWDIESAKVSRILANHMDSVSSSAF
                     SPDGKHIITGSYDRTARLWNADPLHDARTIYGHVDPINSVSYSPDGTNILSASRDNSV
                     RVWNAATLELEDTVKQSEALLDPRAVFSAGGRWLAAGSEDYQGPNANIAFWEKIGGEY
                     PKPVVVPTNDNHVSSLVFSPPGDGGESEYLLTGYENGQLDLWQNVDGVWNTVRTVVEP
                     YGGPSFPTVMFTSDSKYIASVNGKIKIINIQDPNEVVSSCAEELFSGTTYNAKTIAFS
                     PDSKYLAVPLGNDVVILNWKTNQRQGCTLVGRLNGHLAPVKGLIFSPDGNYLMSTSED
                     GIAILWGTETWQQLRLLAGHEGVITSAAFSPDSKSIVTGGADKTIRIWDVDYMDAVRQ
                     VCSILLNLGRDFTEPERSKYGITDEKPTCNQ"
BASE COUNT         2842 a         2517 c         2706 g         2665 t
ORIGIN      
        1 tggctttctg atcatcaaca cgatcaccgc gctgatgacc cagcagaccc gccaggtagg
       61 aattatgaag gctacaggtg gcggcaccag ccagattttt gggatgtata tcgttttgat
      121 cgtttgcttt gggctggcag cgctcctgat tgctgtaccc ctcgcgaatt tgtcagcaca
      181 ggcgattggt ggtggtatgg caggctggtt gaactttcat ccaagtccgt ataagggtta
      241 tgccgcaacg cttttgcagc aattttttgt ggcggtgatt attccgttga ttgccgcaat
      301 tttcccgatc tataatagtg ttcgggtaac agttcgcgag gctttgagcg attatggatt
      361 gggcgggaac gccaaggtca aagctgattc tgtaaataag aactcagtgc ttattccacg
      421 cccgattcgt atttcgttgc gaaatacgtt tcgccgcaaa acgcgtctgg cactcacttt
      481 gttcacgctg gtacttggtg gcgctgtttt tatcgcagtt tataaccttt gggcctcctt
      541 cgataaggtc atagaggaca ttcaaggtta ttttctggcc gacattaaca tcagcttcaa
      601 ccgcgcctac cgcttcgatg aagtagcagg gattgcgcaa aacgtgccgg gcgttgaagg
      661 cgtggagggc tggctcgagt atgcaggtac gttgattgca gatgaaacgg cggcaggtac
      721 acaaatccta ttcgttgcgc ctccttccga ctcgacgttg attgacccga ttatcacgaa
      781 gggccgctgg ttgaagacgg gtgacgaaaa cgccatcgtg atcggaaacc atttactgca
      841 aatgttccca gatctgcaga ttggcgatga gctcatcatt gaaaccaacg gtaaaaaatc
      901 tccgtggaag attgtgggct tctatagtat tacaggcaat gtcagcccgc cactcttata
      961 tgtaaattat gaatacatca gccgcctgat caaccagccc ggtcaggttt attcgctgcg
     1021 agtgatcacg gcgagccgcg atggagattt tcagcggcgc atcaacgacc agctacaggc
     1081 agcctatgaa tcccacggtg tgcaaatcgg ttcatcgcaa ttggggtcgg agtttatcga
     1141 aagccaaaaa tcgcaaactg atatattagt ctactttatg cttgtgatgg caagcctgat
     1201 cgcaatcgtg ggtgggctcg gtctgatggg cacgatgagc attaatgtac tggagcgtac
     1261 tcgtgagatc ggtgtgatgc gtgcgattgg cgcgtcgaat ggcgatatcc aaagcattgt
     1321 catcatagag ggcatggtaa ttggtctgct gggctgggca atcgccattt tatttgcggt
     1381 tcctatcaca tcagtactga ctacaggcgt tggtttagca atcctcaccg ctcctatgcc
     1441 agctgtttac gatgtaaacg gaattattgc ctggcttatt tttacgttag tgttggccac
     1501 gattgcaagc acactaccgg cgcggagagc atccagattg acggtgagag atacattggc
     1561 ttatgagtga aagctgagcc gaatcgcata gtacatccgt agcaacgcca aaaactgaca
     1621 attgaatctc cattgcagct tgtttcgtcg tgtgaggtcg aaatgttcaa agagttataa
     1681 tctccataaa ggagatctac atggggcgca ttgataaaac cgtttttatc agttaccgaa
     1741 acacaaatca gccctgggcg ttagcgatct atcagcatct catccatcaa ggcttcaatg
     1801 tgttctttga ttacaatatc cttaagagtg gggattttga acaggtcatt acggaaaata
     1861 taagagggcg cacgcatttt gttgtcttat tgacaccgtc cgcattggag cgttgcaacg
     1921 agcccggaga ccagatgcgc cgcgagattg aattagcgtt agacgaaaag cgcaatatcg
     1981 tcccactctt cttcgaaggc tttagcttta gtagtccatc catttccaaa tatctgacag
     2041 accgacttga cctcctgaag aaacacaaag aaatcaccct gtcagccgaa tacttcgacg
     2101 cggctatggc tcgattgtgc aatgattttt tgaacgttcc atttgctgcg attttgcatc
     2161 cactatctga tacggttgaa aacgccgtca agatgcaaca ggctgctgcg aggaaagcca
     2221 aaaagataca acataatgaa ctggcagcac aggaatggtt tgagcagggc aaacattacg
     2281 atgacagtaa cgatttcgat gaatgtatca atagttatac aaaagccatt ggactcaaat
     2341 ctgattatgt tgaggcatac cttagacgtg gcgtggcgtg tcacgagaag ggcgatatgg
     2401 acggcgcgct caaggacacc aatgaagcca ttcgcctcaa ccccgatttt gcggaagctc
     2461 attttatccg cggacgtgct cgctattaca aaagcgatgt ggagggggcc attcaggact
     2521 ataacaaagc tatccgcctc aatccagagt atgcgttagc ctactacaat cgcggcattg
     2581 cgcgcagcga taaaggagat ctggctggtg cgatcaagga ttacggtgaa gccattcgct
     2641 tcagcccgga ctatgcgtta gcctactaca atcgcggcat tgcgcgcggc gataaaggcg
     2701 acctggcggg tgcgatcaag gattacagcg aaaccattcg cctcagccca gagtatgcgg
     2761 atgcctataa caaccgcgca attttgcgca gtgacaaagg agacctggct ggcgccctcg
     2821 aggattacaa cgaagccatc cgactcaacc ccagtgatgc ggatgcatat ataaatcgag
     2881 ggattctttg ggaagaatat aagaataatt acgatgcagc aatcgccgat tttcaaaggt
     2941 atatcgatct cggtggggaa agaaaggaag ttgcagagtg ggtcacggat ttgaaaaaga
     3001 aatcgaatag cgaatagaac gcctggaatt acatatcccg cggttctttt agccacgccc
     3061 gaaaattcat ctcacaatac tttgtttccg tcctgaaaga atgtaaaatg gggctggaaa
     3121 gtccaggcaa tggatctcca atcccaactc tcacaaatca ttcatccgtc gcgcgtcctg
     3181 acctctcact tgcatcgaat cgcatacgcg aatgatgcgt cgtattttcg gctcctgcct
     3241 caggcagttg ttcaaccgaa ttcgatcagc gaaattcaat cccttttcaa atttacacaa
     3301 caaaataata ttcccatgac cttccgtgcg gcagggacaa gcctctctgg tcaggctgta
     3361 actgacgggg ttctggtcga tatctccaag cattggggaa actacagaat cgaagaaaat
     3421 ggtgagctga ttcgttttca acccggcatt gtgggtggct tcatcaacaa tgtgcttaag
     3481 gggtacggca gacgcatcgg gcctgaccct gcctcgatcg acgcctgcat gatgggcggt
     3541 atccttgcca acaactccag cggaatgtgc tgtggggtga tcgaaaattc gtaccatacg
     3601 attcattcga tgatcctcgt cttacccaat ggattcgttc taaacaccgc agactcgaat
     3661 gcaaatcaga tctttgaaga tcaacaaccg catctagcaa agggcttgct ggaacttaag
     3721 aaaaggattc aggcatcctc cttttccgga aaaggaggga ctaacgggga ggtcgaatca
     3781 ctggccgagc gcatccatcg ccgttaccaa atgaaaaaca cgaacggcta tttgctcaat
     3841 gcgcttctcg aattcgatac cccgctcgac atcctgactc acatcatgat cggctctgaa
     3901 ggaacgcttg ggtttatcgc cgagggcgta cttcacaccc tgccggatat gccgcgccgt
     3961 tatacgggtc aactttactt caagaattta caggatgcag cttcagcaat cctgcccatt
     4021 aaggaatcgg gggcgcgtgc tgtcgaaatt atggaccgtg ccgcgcttcg ttccgtggaa
     4081 aacaacgctg gcgtgccgtc tatactgagg gaattgccag agggcgcgac agccatcctc
     4141 gtggaatatc aggcacagga tgccgaatca ctgttcaggt ccacaaaaga ggcggagcag
     4201 gttatcagga agctaaagct tattcatgat ccggaattta caggcgatcc ggctcaacag
     4261 gcggcgcttt ggaaaacgcg aaaaggaatc atcccatcca tcggcgggat gcgtgcaccg
     4321 ggaacggtct gcatcaatga ggatgttgtc tttccgattc atcgactcgc agaagctgtt
     4381 accgaccttc agcatctttt tgcagatttt ggatataccg atggcgctgt atttgggcat
     4441 gcaaaagacg gcaacctgca tttccttatc aatcaggcct tcaacacaaa cgcggaggtg
     4501 acacacttcg atcgtttcgt tcatgccatg gttcagctcg tcagcggaaa gtacgatggc
     4561 gcgctcaaag ccgaacacgg tacgggacgc aacatggcgc cgttcgtcga aacggaatgg
     4621 ggctctgaag cctatgccat catgcgtgat ctcaaatcct tgttcgatcc tgattacatg
     4681 ctcaaccctg gtgtgatgat caaccatgac ccacaagcgc atgttcagca tctcaagacg
     4741 atgtcactcg tgtcgcctga agtggataag tgcattgaat gcgggttttg cgagtctaaa
     4801 tgcccaagcc gacagctgac ccttacgccg cgccaaagga tagtcgtcca gcgtgaggct
     4861 tcccgcctaa aagcactcgg ttcagactca tcgacacttg aatccatttc aaaagatttt
     4921 tcctacgctg gaatggatac ctgcgctgta gacgggctct gcgccaccgc ctgccctgtc
     4981 agcataaata ctggtgacct gacccgacgc ctgcgctctg aatcaagtac accgggcggc
     5041 aatcaaacgg caaaatggct ggctgatcat ttttcattga ctgaatttgc ggttggctcg
     5101 gctgcacagc tgggtcatat cgcagaatcc atgcttggtg taaaaggtat caattcagtg
     5161 attcaaacgg ctgaaaagat ttccaaaacc acgttaccca agtggaattc tcacattcca
     5221 tccccgtctc ctaaacggag agaggttagg ggtgaggtca acaaggactt catctacttc
     5281 ccttcctgca tctcgaggca attgggtaaa ccaaaaggtg gcagtcactt atcgctacct
     5341 gaaactctta ttaccctcgc caaccgctca ggcatttcac tttggattcc acctgatgca
     5401 aacggcacat gttgcggcat gccttttcat tccaagggat ttgaatcggc atatcacgcg
     5461 tcacttcaca agaccttcgc caatctttgg gaatggtctg agcagggaaa atatccgatt
     5521 gtcgtagata ctacctcctg cacacatacg ctgcgtacat gtcaggaaga cctggacacc
     5581 gaagataagg aaatctggag taacctggct ttcctggacg gtctggattt tttgaatgat
     5641 tttgtattac ccaaacttgc agttcatccg attgatgaag atatcatcct gcaccccaat
     5701 tgttctgcaa ggaagcttgg gctggattca aaaatgctgg caattgctaa acaatgtgca
     5761 agatcggcaa ctgttcctct gcatctcggc tgctgtgcgt ttgcaggtga ccgcggactt
     5821 ctctttcccg aattgacacg atccgcaaca gaaaaggaat ctgctgaagt caatacgcgt
     5881 gaatatggcg gatattattc atccaacatc ccctgcgaaa ttggcatgtc tgaagctact
     5941 gggaaagatt acacgtcgat tgcctattta gtagaaaggg catccagaaa acaagatgaa
     6001 aaagaaagcg ttcgatattg accttgcact ggaaggtatc agcgaagccg tcaaaccctg
     6061 gcccaaggcg gccatgtttg aactggcaga ggagggcttt gactcgccgt tcgaacaact
     6121 ggtcgcttgc attatctcca tcagaacgta tgatgaagtt tcattaccaa cggcacgagc
     6181 attgttcgcc cttgcgcgca ctcctgcaca gataagcaaa ttaagccctg aggaaattga
     6241 cggactcatc catgcgtgta cgtttcatga acgcaaagct gcacagatcc atgcgattgc
     6301 aaaacgtgtc gtggtggaat atgaaggaaa cttgccgtgc gaggctgagt tactgctttc
     6361 tttctcaggc gtcggtccga agtgtgccaa tttagcatta gggattgcct gcggacagcc
     6421 agcgatcagt gtagatatcc acgtccaccg cgtgacgaat cgctggggat atgtaaaggc
     6481 atccacgccc gaaaagacga tgcttgctct tgaagccaaa ctcccccgcg aacactggat
     6541 taacatcaat cgtcttcttg ttccttttgg taaacatatt tgtacgggta caatgccaaa
     6601 atgctccacc tgccctgtgc tggacatgtg tgcgcaggtt ggcgtcaaaa ctcatcgatg
     6661 aaaaactaag atgaacattt ttattacagg cgcatcaggc ttcattggtg gtgcaattac
     6721 aaaagcttta gctgcgtctc acaacgttta cgggatgtcg aggtcgaatc agagcgatga
     6781 ccatatccgc tctttgggag cgacgccggt ccgctgtgag ttgggcgcag tgagacccga
     6841 gcatctcgca gattgcgagg tcgtaatcca ttgcgcagcg ttcgtcaaac agtgcggcac
     6901 gcgggaggaa ttctggaaga cgaatgtgga tggaaccgcg caactgctgg aagtggctcg
     6961 gctggcaggc gtgaggcgct tcatccatgt gggtaccgaa gccgcgctgt ttcacggaca
     7021 acacatgcgt gatatggacg aaacatatcc atatccggac agcaccccat ttctctatcc
     7081 cgaaacaaag gcggcagctg agaaattggt gctttcagca aatgcccccg gctttgagac
     7141 gatctcttta cgtccacgat ttgtgtgggg accaggtgat cagaccattt tgcccgtggt
     7201 caagaagatg gtgaataaag gaatgtacat gtggatcgat cagggacgtg tccgcacatc
     7261 cacgacgcat attgacaact tcgttcacgc tgtgacatta gctctgacgc gcggtcgtgg
     7321 tggagaggca tacttcatca ccgatgactc cacgtcaacc gttcgagaat ttctgacggc
     7381 attattgaag acgcaggcaa taacaatgcc aaacacatcc attcccagct ggcttgcgcg
     7441 ggcgttagga tggagcatcg aaggcatttg gaaactactg aggctcaaga gcgagccgcc
     7501 gttgacgcac ttcgccgcag ccatggcatc cagtgaatgt acaatccgca ttgacaaagc
     7561 gcgggctgaa ctgggctatg caccagtcat gaccattgat cagggtttag cagaattagc
     7621 aaatccataa tcaggaactc ttttctggta tcagtgtttt gttgattacg aggggcacca
     7681 cagaaacgtg gagtagaagt tcagggtaag gagatagata tggcaaaagt cttcgtttcg
     7741 tattcacgta aagacatcga attcgcaaaa cgactcaccg ccgaactcca aaaaagcgac
     7801 ttggatttct ggatcgattg ggaaggcatc ccacccaccg tggattggtg gagggagatc
     7861 gagaaaggca tagaagaatc ggatgtcttt attttcctta ttagtccgga ctctgcaaaa
     7921 tcaaaaattt gtgggcagga aattgatacg gctgtcaaaa acggcaagcg attgattcca
     7981 ttggtggtgc gagatatcaa aggggatgag acgccatcac agctcagcca tctgaactgg
     8041 attttcttcc gcgaaggtga tgattttgac gccggtctcc aaaaactcgt aaccggcatc
     8101 cataccgatt atgaatgggt aaaggaacac cgctggttac aagtgcgggc actggactgg
     8161 gagcgagaaa ataaagacag cggatcattg ctgcgcggca aagatttgca agatgcagaa
     8221 tttcagctgg ctacaaacac atcgaaagag cctcatccta cggatctaca gcgcgaatat
     8281 gtattcacaa gccgcaccgc tgctgaccga caacgtaggg ttactatggg cttttcgatt
     8341 gccgggataa tcgctttggc aacccttgcg atttggggat ggggccaggc cggtcaggcc
     8401 acactcaacg cagatgaagc gaacaagaat gcggcaatcg caaatgcagc aagtacactc
     8461 gcgtttgaaa atgcgatcag agcagatcac gaagctgcca ttgctcaagc tgccagcacg
     8521 tttgcggttt ccaaggaaca ggaggccgaa agacaggcgc aaatttccct ggctcggcaa
     8581 tgggcggctg aagctaattt tctcctcgca aaccaaaatg gaaatacaga aacagctgca
     8641 ttgctgagtt tgcgagcgtt gcagttacac gatgtgtatt taccatccgc agatgcggcg
     8701 cttgtggcga gtttgaataa actatatacg gtgaagattt ttgatgagca tgagtcaaag
     8761 gtcagcgctg ttgcatattc gcccaacggg gatcgaattt caatcggtta tggtgatgga
     8821 accgtagggc tatacgacct gcctgtcgga aaagaaataa ggcacttcaa tgtagaattg
     8881 agagattacg aaggcgatcc gtatgagaca ggcatacatg cactttcatt ttccgccgac
     8941 ggtaattctc ttctcatcgc tgattcagga ggacgtgttg tggtatggaa tctggtaact
     9001 ttcacaatag aagttgattt ttatgttcaa gaaaatctcc ctctttggtc cgcgaagttt
     9061 tcacctaatg ggcaattcat tttgatcagc actggatttc cccacagcgg tccgggcaac
     9121 atatatttgt ataatcttca gaatggcgaa aaagcaaata tttcatttgc gcagcttcag
     9181 cagcgatatg ttaccgatgt ggaattttcc agcgacggca gccaagtgct ttcaacctca
     9241 tgggataagt cggcaatttt atgggacata aattgggagt ctttagaagc aactccaata
     9301 caaactttcg aattgtcttc gccgattcgg gatggggcaa tttctgcaaa tggcgagttg
     9361 attttgctgg gaacagataa taacagcgct gaactttata cttcaggtgg tgtcttgaaa
     9421 cagacatatg ttggtcatac cagttatgtt tactcagtaa atttttcgcc tgacggaagg
     9481 catattttga caggcagttg ggataaaact gcgcgtttat gggatatcga atctgcaaaa
     9541 gtcagccgta tcttagctaa tcacatggat tctgtttctt cttcggcctt ttctccagat
     9601 ggaaagcaca ttattactgg gagttatgac aggacagcca ggttgtggaa cgcagatcct
     9661 ttgcatgatg cacgaaccat ctacggacat gtagatccga ttaattccgt ttcgtattct
     9721 cccgatggaa ccaatattct ttcagccagc cgcgataaca gcgttcgcgt gtggaacgcg
     9781 gcaaccctgg aactggaaga tacggtcaag caatcggagg cattacttga tccccgagcg
     9841 gttttttcag cgggcggacg ttggcttgct gccggaagtg aggattatca ggggccaaat
     9901 gcgaatattg ctttttggga aaagatcggg ggtgaatatc cgaaaccggt ggttgttcca
     9961 acgaatgata atcatgtgtc gtcgctggta ttttccccgc caggtgatgg aggcgaaagt
    10021 gaatatttat taacaggcta cgagaatggc caattagatt tatggcaaaa tgttgatgga
    10081 gtttggaata cggtaaggac cgtcgtcgag ccatacggtg gcccatcatt tccgaccgtg
    10141 atgtttacct cagattcaaa atacatagcc agcgtgaatg gaaagatcaa aattatcaat
    10201 attcaagatc ctaacgaagt agtttcaagt tgcgctgaag aacttttttc aggcacaacc
    10261 tacaatgcga aaaccattgc cttttctcct gactcaaaat accttgcggt gccccttggg
    10321 aacgatgttg ttatcctaaa ttggaagaca aatcaaagac agggatgcac actcgtcggg
    10381 aggcttaatg gtcatttggc accggtgaag ggattaatat tctcccctga tggaaactac
    10441 cttatgtcaa caagcgagga tggaattgcc atcctttggg gtaccgaaac ctggcaacaa
    10501 ttaagattac ttgcaggtca cgaaggggtg atcacttctg cagcattttc tcctgatagt
    10561 aaatcaatcg taacaggcgg cgcagacaaa actattcgga tttgggatgt tgactatatg
    10621 gatgcggttc ggcaagtatg ctcgatcctt ttgaatctcg gaagagactt taccgaacca
    10681 gaacgtagta aatacggcat tactgacgaa aagcctactt gtaatcaata
//
LOCUS       DASTJP010000831         8379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_133734, whole genome shotgun sequence.
ACCESSION   DASTJP010000831 DASTJP010000000
VERSION     DASTJP010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133734"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFQ23_24245"
     CDS             complement(<1..754)
                     /locus_tag="VFQ23_24245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909781.1"
                     /translation="MRSRSVILLLLISALLAACGGAAESSVTATPIPTDTQIVPPTLT
                     ATPTIPLAVLVLPAELDPDTSNLYQTTVYDLAQASGYRFQVRNTLTPADIEPGLKVVI
                     ALPPDPGIVALAAAAPNVQFLAINIPDVVAGGNISVLGNNSQSDIAAFLAGYTAAMIT
                     DDYRIGMMIPKDNPDAIRSLNAFANGMKAYCGICRPLFFYQSTFPQYIEIGALEDPAN
                     YDAYADILMLQYKVGTIYLHPDIATTDLMNYIG"
     gene            complement(942..1322)
                     /locus_tag="VFQ23_24250"
     CDS             complement(942..1322)
                     /locus_tag="VFQ23_24250"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9909782.1"
                     /translation="MTFYLHPYPYRRAARRLVANNERALGVNIREQDDAYVLSALVPG
                     LNAEDLNIQVLEDVVRIEGEYKADETQYLMQELPHGSFNRTLRLPTVIDAENVEAKIT
                     DGVLTLTLPKAESARPKKIKITSK"
     gene            complement(1476..2867)
                     /locus_tag="VFQ23_24255"
     CDS             complement(1476..2867)
                     /locus_tag="VFQ23_24255"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9909783.1"
                     /translation="MDRKIVRMLSLVLVFVLALSYSPAFAASQNGMIGINVVFNTDVT
                     DALLADLGTHGRVRDVVYEIDAVTLQARASELAAIRALSYVAAANPDAEVKGVPVDTV
                     AATNFVNGVNTWDQDAVNVTNLQAGPDRTISYDGTGVYVAVLDTGLLYSWRQYFPQER
                     IAEEFAVALGGGGGTQGTVSTQPNKWQQDTNSHGTHVTSTILGYSLNGVPVNGTAPMA
                     TVIPVKVLNQNGSGQNSVVARGIVYAANLKANGDLGSSPLVINMSLGDSVPDAMIEAA
                     VDYAIAHGVIVVASAGNAGETGMGWPGAYPQVISVAASGWIGEWSPPGNNGWWNNLDV
                     PDPTNPADYYITDFSSRELAGQDLDVAAPGSWVVGPYQTQQGKTSYFFLGGTSMASPH
                     VAGIAALMLQKNSSLTQAQVENILETSAIPLAPGCRNVLTPFGPNPEQFCWGADATGS
                     GLATADAALAATP"
     gene            complement(3234..4085)
                     /locus_tag="VFQ23_24260"
     CDS             complement(3234..4085)
                     /locus_tag="VFQ23_24260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9909784.1"
                     /translation="MSRKLLVVVFSVMVVLAVAVSPVGAITNNWVNDFEHPFVGLVVF
                     YDEAGEFSHRCSGSLISPTEFLTAGHCTDGATTARVYFQQDAGANFNGTIDPVSGYPE
                     TCAAGTLGTLCATSDELYNYGFNDFAGFPNTHDAGMVILDQPIALTEYGVLASAGALD
                     NLGTARGTKNTVFTVSGYGLTLSTNVNDANPNISFRSRLMAASTLVNLNSGNNDGYNL
                     QTQGNGNGRGGTCSGDSGGPVFLGGPESNVIVAVTSFGLNALCRGTDFAYRIDRQEVL
                     DWIANPE"
     gene            complement(4383..5195)
                     /locus_tag="VFQ23_24265"
     CDS             complement(4383..5195)
                     /locus_tag="VFQ23_24265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9909785.1"
                     /translation="MRKKMLFVVFSVLVILAVAVSPVGAVTDGQPDGNGHPFVGLMVA
                     QDADGNPLWRCSGTLLSDTLFLTAGHCTEAPAAHVEIWFDADVESGIPANGYPFNGDV
                     GGTPHTHPDYNPNAFFVRDVGVVVLDTPFASPNGEYGELPSLNQLDSLKTKRGKQNQM
                     FTAVGYGLQESFPDAAAWQENNVRVRMVAHPHLIQINVPGFTGDFSILLSNNANTGGT
                     CFGDSGGPNFIGNSNVVGGVTSFGINGNCAGTGGVFRMDRSWSLDWVGSFLN"
     gene            5742..6059
                     /locus_tag="VFQ23_24270"
     CDS             5742..6059
                     /locus_tag="VFQ23_24270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909786.1"
                     /translation="MSTSIPTPADANLQNLVGKARADLANRLAVSTNEINLIEATSVT
                     WPDSSLGCPQEGMVYAQVLTAGYLILLEQGGTTYEYHASGGDTIVTCDNPSSPVPGSP
                     GNT"
     gene            complement(6178..>8379)
                     /locus_tag="VFQ23_24275"
     CDS             complement(6178..>8379)
                     /locus_tag="VFQ23_24275"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9909787.1"
                     /translation="GGNYGVATTGPSTAIGSSISGIAPRARIAMYKALWSTQDASTAS
                     GNGADLVAAIDQAVADGVDVINYSISGTSTNFLDGAEVSFLFAADAGVFVAASAGNSG
                     PTASTVAHPSPWITTVAAGTHNRSASGSVTLGNGATYFGASVAAGVGPAPLIDSIAAG
                     MSGASAAAVSLCFSTATNGGTPALDPALVAGKIVLCDRGTNARVDKSFAVAEAGGVGM
                     ILVNTSNNSLNADLHSVPSVHLSHLDRPAVKAYAATPGATATINPAAFNFSTPAPFTA
                     AFSSRGPLTAGGGDLLKPDVIAPGQDILAAVAPPGNFGLSFNVLSGTSMSSPHVAGLA
                     ALLMDLHPDWSPMMIKSALMTSSGDVLDGSNTSPAVIFAQGAGHVRPNSAADPGLVFN
                     HGFNDWLAFLCGTTTGVNPASCTALANAGYSFDPSNLNGASIAIGDLAGIQTVTRKVT
                     NVGSSAATYNASVTGMAGFNVSVSPSSLNLNPGQTGTFTVTFTRTTATLGSYVGGQLT
                     WTDGTHNVRIPLVARPVALAAPRQVNGTGGAINYNVTFGYTGPFTATARGLIPAVTTT
                     GTVTDDPTDSTCSLTSPNAQLLPVTLPAGTTYARYSLFDVDVNPGTDIDMCLFQGTTL
                     VGSSGSGTSAEEINLTFANPLAGNIPLTLVVQGWGVVGSSPFKLHTWYLGTAAAGNMT
                     VTAPASATIGGTGSINLTFTGLAPATRYLGSVVYGGSTGLPSPTIVRVDTP"
BASE COUNT         2156 a         2111 c         2203 g         1909 t
ORIGIN      
        1 tgccaatata gttcattaaa tctgtcgtcg cgatatccgg atgaaggtag atggtaccga
       61 ctttgtattg caacatcagg atatctgcat aggcgtcata attggccgga tcttccagcg
      121 cgccgatctc aatatactgc gggaaagtcg attgataaaa aaagagcgga cggcaaatcc
      181 cgcaataggc tttcataccg ttcgcaaagg cattcaacga acggattgca tcgggattgt
      241 ccttcgggat catcatgccg atacgataat cgtcagtgat catggcggcg gtatagcccg
      301 ccaggaatgc agcaatatca gattgtgaat tattacccag tacactgata ttgcctccgg
      361 caaccacgtc tggaatattg atggcaagga attgcacatt gggcgccgcc gcggcaagcg
      421 ctacgatacc agggtccggc ggcagagcga tcactacctt tagccccggt tcgatatcag
      481 ccggggtcaa tgtattacga acctgaaatc gatacccgga ggcctgagcc aggtcataca
      541 cggttgtttg gtacagattg gatgtgtctg ggtcaagctc tgccgggagc actaatactg
      601 cgagcggtat agtgggggtg gcagtcaggg tgggaggaac gatctgtgtg tccgttggga
      661 ttggagtggc tgtaacagat gattcagcag cacctccaca ggcagccagc aaagcggaaa
      721 tgagaagtaa caggatgaca gatcttgaac gcacaggctt ctccagtcgt aagatggacg
      781 aattatacac tttgctaact gatggtcttg ctcgttactg agcttttgaa atgaactgaa
      841 ggctataact cgcagttaga ctgtaggtcc ttatgcggca ctccgaaaaa caaaaaagcc
      901 cgtgctcaaa gcatgggctc tttagggtaa tttttctctt cttacttgga cgtgattttg
      961 atcttcttcg gccgggcgga ttctgccttg gggagcgtca gtgttaacac gccatctgtg
     1021 atcttcgctt caacgttttc cgcgtcgata acagtgggca ggcgcagggt ccggttgaag
     1081 gagccgtggg gaagttcctg catgagatac tgggtctcat cagccttgta ttcgccttca
     1141 atgcggacga cgtcttccag gacctgaatg ttcaggtctt cagcgttgag tccgggcacc
     1201 agcgcagaaa gaacataggc atcatcctgc tcgcgaatgt tcacaccgag ggcgcgttca
     1261 ttattagcaa ccaggcggcg ggcagcgcgg cgatatggat aggggtgtaa gtagaatgtc
     1321 atggtaagtc tcctaagtaa agaacttgat tgaatttttt cctagtgtag tgagtgagta
     1381 taagaaatga aataaagcga catagaattt ttataagaaa aatcccccgc attcgttgcg
     1441 ggggatagat gcatactcac ttcgtacaag ctgatttatg gagtcgctgc gagtgcagca
     1501 tccgcggtcg ccagacctga accggtcgca tctgcgcccc agcagaattg ttcagggttc
     1561 ggaccaaaag gtgtcagaac attgcgacaa ccgggcgcca aaggtatggc tgaggtttcc
     1621 agaatattct caacttgagc ctgtgtcagg gagctattct tctgcagcat gagagctgca
     1681 ataccagcga catgcgggct ggccatgctt gtgccgccga ggaagaagta acttgtcttg
     1741 ccttgctgag tctggtaagg accaaccacc caggaaccag gtgcagctac atccaggtcc
     1801 tgacctgcca gctcgcggct ggagaagtcg gtgatgtagt agtcagccgg gttggttgga
     1861 tctggcacat cgaggttatt ccaccagccg ttgttgccag gaggtgacca ctcaccgatc
     1921 catccggatg cggcgacaga aataacctgc ggatacgcgc cgggccagcc cattccggtc
     1981 tcacctgcgt taccagccga ggctacgacg atgacgccat gtgcgatcgc ataatccact
     2041 gctgcttcga tcatcgcatc tggaacggag tcgccaagcg acatgttgat aaccagcgga
     2101 gaagatccca aatcaccgtt ggctttaagg ttagccgcat agacgatgcc gcgcgctaca
     2161 acagaatttt gaccagagcc gttctggttg agcactttga caggaatcac ggttgccatc
     2221 ggagctgtgc cattcaccgg cacgccattc aagctataac ctaaaatcgt gctggtcacg
     2281 tgtgtgccat gcgaatttgt atcctgctgc cacttgtttg gctgtgtaga aacggtgccc
     2341 tgtgttccgc caccaccacc caaggcaacg gcaaattcct ccgcaatacg ctcctggggg
     2401 aaatactgac gccacgagta gagcagacct gtatcaagca ccgccacata aacacctgtg
     2461 ccatcatagc tgatcgtgcg atcgggaccg gcctggagat tcgtgacgtt gaccgcatcc
     2521 tgatcccagg tattgacccc attcacgaaa ttcgttgcag cgaccgtatc tacaggaacg
     2581 cctttgactt ctgcatccgg gttagcggct gcaacatacg atagagcacg aatcgcggct
     2641 aattcgctgg cccgagcctg cagtgtgacc gcgtcgattt cataaaccac atcgcgcact
     2701 cttccatgcg tcccgaggtc ggctaagagg gcatctgtca catcggtgtt gaaaactacg
     2761 ttgatgccaa tcatgccgtt ctggctggca gcgaaggccg gagaatagct caacgccaga
     2821 acgaatacca acacaagtga aagcattcta acaatttttc gatccatggt tgtaaagctc
     2881 ctctttagat taggtagagt aaaagttgca actacacttt cttattcgtt tgggcaggca
     2941 cctcctccaa aaatcaagaa cgaccagcac tgaaatagcc tcgacgatct gcacaagttg
     3001 ttcgcctcag ctcgccagat ccatggcttg agattcctcc cgacgatggt tatggttcca
     3061 acggagcgcg agtctaattg aatgacggtt actttgggtt aaggggaaat gagaaaatcc
     3121 ctggcggtaa tttgggatga acataggaaa aacattaagg aagcccatgt acatgacacg
     3181 ggctttatta tgctgtttga gtttattccc catgctaagc gtgctgaaga taactactcc
     3241 ggattggcaa tccagtcgag cacttcctgt cgatcaatgc gataggcaaa gtcggtcccg
     3301 cggcaaagag cgttcaagcc gaatgaggtg actgccacga taacattcga ctcgggtcct
     3361 ccaaggaaga cggggccacc ggagtcaccc gagcacgtac caccgcgccc atttccattg
     3421 ccctgggtct ggaggttgta cccatcgttg ttgccactgt tcaggttaac cagtgtggaa
     3481 gctgccatta gtcgcgagcg gaaggatatg ttgggattag catcatttac attggtgctc
     3541 aacgtcaggc cgtagccact tacggtaaag accgtattct tggttccacg agcggtacct
     3601 agattatcca atgcgccagc ggacgccagg acaccgtatt cagtcaatgc aatcggctgg
     3661 tcaagaatca ccatgccggc atcgtgtgta ttggggaacc cggcaaagtc gttaaagccg
     3721 tagttataca gctcatcaga ggtggcgcaa agcgtaccca atgttcccgc cgcgcaggtt
     3781 tcgggatagc ctgagaccgg atcaatcgtg ccgttaaagt tggcgccggc atcctgctgg
     3841 aagtacacac gggcggttgt tgcaccatcg gtacagtgac cagcggtcag aaactctgtt
     3901 gggctgatca aggaaccgga gcaacggtgg ctgaattcac cagcttcatc ataaaaaacg
     3961 acaagaccaa cgaacggatg ttcaaaatcg ttcacccaat tgttggtaat cgctcccaca
     4021 gggctaacag cgacggcaag gacgaccatg acggagaaca caacaactaa caattttcta
     4081 ctcattgact aactccttat aaaagtttga gttgggttga aaaccagaga tgtgcgatga
     4141 atgagtccag tcacctcctt tgaagcctga gatttactcc tcaaaattaa aatgaacccg
     4201 ccttctctgg ctgagattat agttccagat agttggttaa atatgcctct agagaactgt
     4261 tttggttaca atctatcagg atacccagga tactaaatct gcttggaaca taaatcaaaa
     4321 agaaaggaag cccgcgctga gcgcgggctt ccttgttaaa gtactgtgtt ttcctatcag
     4381 tcttagttga ggaaagaccc aacccaatca agggaccagg aacgatccat gcggaagacc
     4441 ccacccgttc cagcacaatt gccgttgatg ccaaaggagg tgacgcctcc aacgacattg
     4501 gaattgccga tgaaattggg accaccagag tcaccaaagc aggtaccacc ggtattagca
     4561 ttattggaga gcaaaatcga aaaatcgccg gtgaagccgg gcacgttgat ttggatcagg
     4621 tgagggtgag cgaccatgcg aacacggaca ttgttctcct gccaagccgc cgcatcaggg
     4681 aaactttcct gcaaaccata gccgaccgca gtgaacattt ggttctgctt accgcgtttg
     4741 gtcttgaggc tgtcgagctg gttcaacgaa gggagttcgc cgtattcgcc attaggactg
     4801 gcaaatggag tatccagcac caccacgccc acgtcgcgga cgaagaaggc attcgggttg
     4861 taatctggat gggtatgggg agtgccaccc acatcgccat tgaacggata cccattggct
     4921 gggataccag attcaacatc ggcatcaaac cagatttcca cgtgcgccgc cggggcttct
     4981 gtacagtgac cggctgtcaa aaagagggta tctgaaagca gcgtaccgct acagcgccac
     5041 agcgggttgc catctgcgtc ctgagcaacc atgagaccaa caaaggggtg accatttccg
     5101 tccggctgac catctgtcac agcaccaaca gggctaacag caacggccag tattaccaat
     5161 accgagaaaa ctacaaaaag cattttttta cgcatgtaat gtttcctttt acttttttgg
     5221 aatttgggtt acgttggatt gaccaatttt taccaagata gattcttctt gaacacatcc
     5281 tcctttcaaa agaaccgcaa agtaaaatct gccgaagatt tcttgaatcg acagtctggc
     5341 aatctaccaa gctctacgca tcagtttggt agatcactag ctttgcgttt ccgtcttgcg
     5401 acggtttttt aggttgaact ctaataacat tatattttcc tttttggaaa agacaataac
     5461 gattcatatg attctcatta gacaatgtta caaggtgtag aacagcgtga cgtatacgcg
     5521 atatgtgatg cgtaagctat tcaaccacca cgctgtatag gaaccttttc agccaaccat
     5581 cgtctggaaa gcgttcggag atttctatgc gaattattat tctattgacc gtattttgtg
     5641 catttacatg cggatgtcag gccagtgcca ttgatccggg gtcatcacaa gaaacaattg
     5701 ccagtcccct agcccctccc gcggaaggag atgtaaccgc aatgtcaaca tcgattccaa
     5761 cacccgcaga tgctaacttg caaaacttag tcggaaaagc aagggctgat ctcgccaatc
     5821 gattggcagt ttcgacaaac gaaattaacc ttatcgaagc gactagtgtc acgtggcccg
     5881 attcaagttt gggatgccca caggaaggca tggtatatgc gcaggttttg acagcgggct
     5941 atcttatttt gctggaacag ggtggtacta cctatgaata tcatgccagc ggcggcgata
     6001 cgattgtcac ttgtgacaat ccatcttcgc ctgttccggg cagtcctggc aatacctgag
     6061 cctatactat cgtgctaacc ccgcttagac gacactggca cctctctaac aaaggaacag
     6121 gctggcagaa atttctgcca gcctgtcttc tagcttatag aacaaacacg aacttaatta
     6181 gggtgtatcg acacggacaa ttgttgggct cggcaatcca gttgagcctc cataaacaac
     6241 actgcccaga tatctggtcg cgggggctaa tccagtgaag gtcaggttga tggatcccgt
     6301 accaccaatg gtagcggatg cgggtgctgt gacagtcata ttgccagctg cagctgtacc
     6361 taagtaccat gtatgcagct tgaacggcga actgccaaca actccccagc cttggacgac
     6421 aagagtcagt ggaatattcc cggccagtgg gttggcaaag gtaaggttaa tctcttctgc
     6481 ggatgtacct gaaccactac taccgaccag tgtagtgcct tggaaaaggc acatatcaat
     6541 atcagttcca gggttgacat cgacatcgaa cagtgaataa cgcgcgtacg tagtgccagc
     6601 tggaagggtt accgggagta attgcgcgtt tggagacgtc agtgaacagg tgctgtcagt
     6661 cgggtcgtca gtcacagtac cagtcgtagt tactgcgggg atcaacccac gtgctgtggc
     6721 tgtgaatgga cccgtatagc cgaaggtcac gttgtagtta atagcaccgc cagtgccgtt
     6781 gacctgcctg ggggcggcta gcgctaccgg acgtgccacg agcggaatac gcacattgtg
     6841 agttccatcc gtccaggtaa gttggccacc tacgtaggaa ccaagcgttg cggtggtacg
     6901 cgtgaaggtc acagtgaagg tacctgtctg acccgggttg aggttcagcg aactggggct
     6961 cacacttaca ttgaagccag ccatacctgt cacagaggca ttgtacgtgg ctgcgctgct
     7021 accgacattc gtaaccttgc gagttaccgt ttggataccg gcgaggtctc cgatggcgat
     7081 ggaagcaccg ttaagattgc ttgggtcaaa cgagtagcct gcattagcca gcgctgtaca
     7141 agaggcagga ttcacaccgg tggttgtacc acataggaag gcaagccagt cattgaaacc
     7201 gtggttgaat accaaaccag gatcagccgc tgaattgggg cggacgtgac cagctccttg
     7261 tgcgaagatg actgctggac ttgtgttcga accatcaagt acatcaccag aggaggtcat
     7321 cagagcggac ttgatcatca tcggtgacca atcgggatgc agatccatca gaagagcagc
     7381 gagaccagca acatgcgggc tggacatgga tgttccgctc aatacattga agctaagacc
     7441 aaaatttccc ggaggcgcca ctgctgcgag aatatcctgt cccggtgcga tgacatcggg
     7501 tttcaacagg tccccacctc ctgcggtcaa tggaccacgc gacgagaaag cagcagtaaa
     7561 tggagcgggt gtgctgaaat taaatgcggc agggttaatg gtcgcggtag caccaggggt
     7621 ggccgcgtac gccttgaccg cgggacgatc aagatgagat aggtgaacag aaggtacaga
     7681 gtggagatcc gcatttaggg agttattcga cgtattgaca agaatcatac ccacaccacc
     7741 ggcttctgct acggcgaaac tcttatcaac acgggcattc gtgccgcgat cacaaaggac
     7801 gatcttgccg gctactaaag ctggatccaa cgcaggtgta cctccgttgg ttgctgtcga
     7861 aaaacatagg ctcactgcag cagcgcttgc cccagacatt ccagctgcaa ttgaatcaat
     7921 gagtggcgca gggccaacac ccgctgcgac cgaggctcca aagtaagtag caccattgcc
     7981 cagtgtcaca gacccagacg cacttcggtt atgtgttcca gcagcaacgg tagtaatcca
     8041 tgggctgggg tgagcaacag tactggctgt cgggccgcta ttaccagccg aggcagcgac
     8101 gaacacgcca gcatccgccg caaagaggaa cgaaacttca gcgccatcca ggaagttggt
     8161 cgacgtgccg ctgatcgagt agttgatcac atccacgcca tcggcaacgg cctggtcaat
     8221 ggctgcaacc agatcggcac catttccact ggctgtagag gcatcctgcg tagaccacaa
     8281 tgccttatac attgcaattc gtgcgcgggg agcaatacca ctgatagagg aaccaatcgc
     8341 agtcgaagga ccagtagttg ccacgccata gttaccgcc
//
LOCUS       DASTJP010000832         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1453938, whole genome shotgun sequence.
ACCESSION   DASTJP010000832 DASTJP010000000
VERSION     DASTJP010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453938"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFQ23_24280"
     CDS             <1..536
                     /locus_tag="VFQ23_24280"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9909788.1"
                     /translation="GYPLAGDLSRGGFASENIVAGSNMTIEQAITSWYGDEPHTNTMI
                     SDKYTECGAGVAVTGNVIYYCFDVARSTTSQPTGPTPNAPTPATPAKPVSTSAPSGSY
                     VVYVPKTMTSGLRIRKTGSTSGALVRVAAAGEWLEVQEPKASAKAKVGAENKWLKVKD
                     SKGNVGYVAAWLVSESG"
     gene            558..896
                     /locus_tag="VFQ23_24285"
     CDS             558..896
                     /locus_tag="VFQ23_24285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909789.1"
                     /translation="MLSNLIAVLSGLVLLAYWLKGRAARFARAIAQFDVVIGVVALVM
                     GVLELLSLLGILLILAGLVLSASALRSIPSVGKSLSRLGNALDQFRLIIGILILLVGL
                     LNLLSALLRF"
     gene            923..1750
                     /locus_tag="VFQ23_24290"
     CDS             923..1750
                     /locus_tag="VFQ23_24290"
                     /inference="COORDINATES: protein motif:HMM:NF015402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HET9909790.1"
                     /translation="MKPPRTLPPKFLAAMVAVLPLPGSPLYDGDDQRVMEQAMADLEV
                     YQSASVDSILFENDHDLPYIQPPLDERGIALMTKIVKEARGRFSGPIGVQMLEAANIT
                     SLEIAAEADLDYIRVEAFVFAHVGGSGIINGSAGKILRRRKELAADHIKVFADVKKKH
                     GSHSLTIDLDIKDEIMQAEFFLADGVIVTSQFTGINPDKTDLTKARAATKLPVLIGSG
                     MTAENITEYLPFADGFIVGSYFRKDGKFLETLEPERLDRFMNVFTSTRNSILDSAKQ"
     gene            1857..2351
                     /locus_tag="VFQ23_24295"
     CDS             1857..2351
                     /locus_tag="VFQ23_24295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909791.1"
                     /translation="MEDSQSSERTPVPDRWSRLLWAGGIVMLLLIVAAVSGYTWIALY
                     GPCSVNTVESASNSMLDQLELFDEMYQSIPTLSPLELFEPMTQLQQILIDTKKVVVPT
                     CLQTAHLELTTAMESLMRALLAIMESKPESTSAGLFEKSNKHRDNFTAELEWINKCSP
                     FCPR"
     gene            2389..2598
                     /locus_tag="VFQ23_24300"
     CDS             2389..2598
                     /locus_tag="VFQ23_24300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909792.1"
                     /translation="MIGGYCIACLMAGGAVYLNQLFTQGSASQASAGMAAFGDFILFV
                     GIFGILALFPTGLAAYFLLRKFLKR"
     gene            2683..>2926
                     /locus_tag="VFQ23_24305"
     CDS             2683..>2926
                     /locus_tag="VFQ23_24305"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HET9909793.1"
                     /translation="MADVIDYQIFGDDLQLVEIGLDRGEGVRAEAGTMTYMEDGIEMQ
                     TGMEGGLFSGFKRAVTGESFFITTFMNNDQRRRHVSF"
BASE COUNT          730 a          695 c          780 g          721 t
ORIGIN      
        1 tgggctatcc actggcagga gatctttcgc gcggcggctt tgcttctgaa aatatcgtcg
       61 cgggctcaaa tatgacgatt gaacaagcca tcacctcttg gtatggagac gaaccacaca
      121 ccaacaccat gatcagcgac aaatataccg agtgcggcgc gggcgtagcg gtcacaggca
      181 atgtgatcta ttactgcttc gacgtggcac gctctacaac atctcagcct acggggccaa
      241 caccgaacgc gcccacacct gcaacccccg ccaaaccggt ttcaacgtcg gcaccatcag
      301 gcagttatgt cgtctatgtt cccaaaacaa tgacaagcgg attgcgcatc cgtaaaacgg
      361 gcagtaccag cggcgctctc gtccgtgtgg cggctgcagg cgaatggctg gaagtgcagg
      421 agccgaaggc gtcggcgaag gcaaaagtgg gagcggaaaa taaatggttg aaggtcaaag
      481 atagcaaagg aaatgtcggc tatgttgcgg cgtggctggt gagtgagtct ggataagata
      541 agtggtaagt cgatgcaatg ttaagtaatc tgattgctgt cctgtctggg ttggtactcc
      601 tggcctattg gctgaagggc cgagccgcaa ggtttgcgcg ggcgatcgca caattcgatg
      661 tcgtcatcgg cgtcgttgca ctggtgatgg gcgtgcttga gctgctcagt ctgctgggaa
      721 ttcttctcat cctggccggg ctggtccttt ccgccagcgc gttgcgttcc attccgtcgg
      781 ttggcaaatc actgtctcgt ctcggcaatg cactcgatca attcaggcta atcattggta
      841 tcttgattct tctggtggga ctgctcaatc tgctttccgc cttgttgcgt ttttgaccga
      901 aatatccata gaggtgatct tgttgaaacc cccgcgaacc ctccctccaa agttcctggc
      961 agctatggtc gccgtcttgc cgctccccgg gtcgccctta tacgatggcg atgatcaaag
     1021 agtgatggaa caggcgatgg cagatcttga ggtctatcaa tcagcaagtg tcgatagtat
     1081 tctctttgag aacgatcatg atctgcctta tatccaaccc ccattggatg aaagaggcat
     1141 tgccctgatg acaaagattg tcaaagaagc gagaggcagg ttttctgggc ctatcggagt
     1201 tcaaatgctg gaggctgcaa atatcacctc ccttgagatc gcagcggaag cggatttgga
     1261 ttacattcga gtggaggctt tcgtctttgc gcacgtcggc ggtagcggca tcatcaatgg
     1321 ttcagccggg aaaatcctgc gaagaagaaa ggaactggca gcagaccata tcaaggtatt
     1381 tgcggatgtc aagaaaaagc atggctccca ttccctgaca attgatttgg atatcaaaga
     1441 tgaaattatg caggctgaat ttttcctggc tgatggcgtc attgtgacca gtcaatttac
     1501 aggtatcaac ccggataaaa cagatttgac caaggccaga gctgcaacaa aattgccggt
     1561 gctgattggt tcgggtatga ccgcagaaaa catcacagag tatttacctt ttgccgatgg
     1621 ctttattgtt ggttcctatt tcagaaaaga tggtaaattt cttgaaacac tggaacctga
     1681 aagacttgat aggtttatga acgtttttac ttcaaccaga aacagtattc tggactctgc
     1741 aaaacaatga agagcgtaca gtctcagttc gtgattgagc tacagtgggg aaaggtcagt
     1801 gtctggtaga gtgaacgtgc cgagtgcaat cgtgtcatgg gaaaaaggta aacaaaatgg
     1861 aagattcaca atcctctgaa cgaactccgg ttcctgatcg gtggtcgcgt ctgttatggg
     1921 cggggggtat cgtgatgttg cttttgatcg tggcagccgt cagtggatac acctggattg
     1981 cgctctacgg cccctgttca gtgaatacgg tcgaatcagc ctccaattcg atgctcgatc
     2041 aattagagct atttgatgaa atgtatcagt caataccgac cctgtctccg ctggaactct
     2101 tcgaaccaat gacacaactg cagcaaatcc tgatcgatac aaaaaaagtg gtcgtgccaa
     2161 cctgcttgca gaccgcacac cttgaactga ctaccgccat ggaatcctta atgcgagccc
     2221 tgctggccat tatggaatcg aaaccagagt caacctcggc tgggctgttt gaaaaatcca
     2281 acaagcaccg tgacaacttt actgctgaac tggaatggat caacaaatgc tccccgtttt
     2341 gcccccgata agcgcctgag aatgagcgga cttacaaaga ttggataggt gattggtggt
     2401 tactgcattg cctgtcttat ggcaggcggc gcagtttatc tcaaccaact ttttacacaa
     2461 gggtctgcct cgcaagcctc cgccgggatg gctgcattcg gagacttcat tttgttcgtc
     2521 ggaatattcg gcatcctggc gctcttccca acgggtttgg cggcttactt tctcttaaga
     2581 aaattcctga agcgatgatc ctctagcttc gcgacccaac gtccacaact tcgtaaaata
     2641 ctatgataac aatcagaatt tcatgcgaag aggagtgcaa acatggcaga cgtaattgac
     2701 tatcaaatct tcggggatga tttgcaattg gtggagatcg gactcgaccg gggcgagggc
     2761 gtgcgagccg aagccgggac gatgacgtat atggaggacg gcatcgagat gcagaccggc
     2821 atggagggtg ggctattttc cggtttcaag cgggcggtca ccggggaaag tttctttatc
     2881 acaaccttca tgaacaatga tcagcgcaga cggcacgtat cgtttg
//
LOCUS       DASTJP010000833         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_792013, whole genome shotgun sequence.
ACCESSION   DASTJP010000833 DASTJP010000000
VERSION     DASTJP010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792013"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..957)
                     /locus_tag="VFQ23_24310"
     CDS             complement(364..957)
                     /locus_tag="VFQ23_24310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909794.1"
                     /translation="MNKHPLSNLLYIGFLLMLTSCQAAGQPWGVSMFKPGDTIDGMSL
                     TTGAQDAIPLWAYCDPAQSIGNAATMVCAVPRLSKLAIGHFLMPGDDTIAMLDWSEIN
                     WELTIDNQPVDLESFGTYEFALPATSHNPSPIREVFVTFTAWDVVLTDLHPGVHTIQG
                     SARMGSDSYRWVIHLTIEGNDLGGRIPWVGPDIQSVF"
     gene            complement(1228..1740)
                     /locus_tag="VFQ23_24315"
     CDS             complement(1228..1740)
                     /locus_tag="VFQ23_24315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909795.1"
                     /translation="MAKKSDFTSEEWQLILSMPQVASLYLALASPSNPVGLAQELIAS
                     TKSIVETLKTSSGNELMDAVSADIREKVEKRERLEAPLKTTNDLNDMKSQCMQACRQV
                     ATLLSQKAPADAEAYKQWVYQAAEDSANAAKEGGVFGIGGERVSEAERAALNEIAQAL
                     DISVANLSRV"
BASE COUNT          504 a          545 c          497 g          526 t
ORIGIN      
        1 atgaaaggcg atatgattga tctctcaagc ctcccacatt ggggagcggc agaagtgttg
       61 ggggttacga tgatgttaac ggcgcatgac aacaccgtcg tattgaaagt gcggccgacg
      121 gcgagttcgc aaatcactta aaagtttgaa cgtgagcggc gcatccgggc tcggttacac
      181 ataatagcaa ccatccattc gaatcgactg aatatatcca accctttgcc gtttgcaaag
      241 tactctgagc tttgaaggac tgtaccctgt cagtattcaa agtaaatcta cggggaaaga
      301 aagagtgcag gtgtgagcca cctgcacttc tcaaccggtc aagtttcatc ctcgttcgag
      361 catctaaaat acgctttgga tgtcgggccc aacccaggga attcgaccac cgagatcgtt
      421 tccttcgata gtcaggtgta tcacccagcg gtagctgtca gatcccattc tggctgaacc
      481 ctggatggtg tgcacgcccg gatgtaaatc cgtcagtacc acgtcccagg cagtaaacgt
      541 cacaaagacc tcgcgaatgg gtgaaggatt gtgcgacgtt gccggcaagg cgaactcata
      601 cgtgccaaaa ctttccaggt caaccggctg attatcgatc gtcaattccc agttgatttc
      661 agaccagtcc aacatcgcga tcgtatcgtc gcccggcatg aggaagtgac cgatcgccaa
      721 tttcgacagc cggggtacag cacagaccat ggtcgccgca ttgccgatgg attgagcggg
      781 gtcacagtat gcccacagcg gaattgcgtc ttgtgcaccg gtggtcaggc tcataccgtc
      841 gatagtgtca cctggcttga acatggagac gccccagggt tgccccgctg cctgacagga
      901 cgtcagcatc agtagaaatc caatatacaa taggttggat aacggatgtt tgttcatgtg
      961 gacctcctcc tggttgaaaa tccaattgcg gacaccattg gtctaatctt aagattttaa
     1021 agaacgtttg tctgtcactt tggcgacacg agtacaccaa ctcaacctct gaaccataac
     1081 tcgtgttgat cataaatagc gttaaagagg agcggacggg aattcccgtc cgctcctctt
     1141 tattggtaac gactcttctt ggttattata attgccgcaa cagggctcta aaagaaatcg
     1201 taaaaagggc gaccctttgt gagcccgtta aactcggctt aggtttgcga cagagatatc
     1261 gagagcctgc gcgatctcat tgagtgcagc acgctctgcc tcactcaccc gttctccgcc
     1321 aatcccgaag actccgcctt ccttggctgc gttggcgctg tcctcggcag cctgatacac
     1381 ccactgctta taagcctcgg catcagccgg tgccttttgt gagagcagcg tagccacctg
     1441 gcggcaggcc tgcatgcact gggatttcat atcgttcaga tcgttggtcg tctttagcgg
     1501 ggcttccagc ctttcccgct tctctacctt ttcacggata tctgcggata ccgcatccat
     1561 caactcattc ccactgctcg ttttgagtgt ttcaacgatg gacttggtgg aagcgatcag
     1621 ctcctgtgca agacccaccg gattgctggg gctggcgagc gcgagataaa gagatgcaac
     1681 ctgcggcatg ctgaggatca gctgccattc ttccgacgta aaatctgatt tctttgccat
     1741 ttttcatgtc tcctgggaaa tttagaattt cttgaaaaca acaataactc gatccccttt
     1801 cggaacgtgc tctgcacgcg gcaggcatca ggttcactat acgttatgtt gaaccgctcc
     1861 acaacggggt acatcctaca acttcggtag gaataccccc ttaaaaagcc cgccttataa
     1921 ctactatatt ttcattgccc catctttggc acaagtttac atactaagac catcgtcttg
     1981 atcctctgtt aatttgggtt ccaatgaata atgtatatat ggaaaatata aaaaggagcg
     2041 gacagtcatc actgtccgct ccttttcgtt ta
//
LOCUS       DASTJP010000834         8784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1015145, whole genome shotgun sequence.
ACCESSION   DASTJP010000834 DASTJP010000000
VERSION     DASTJP010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015145"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..344)
                     /locus_tag="VFQ23_24320"
     CDS             complement(39..344)
                     /locus_tag="VFQ23_24320"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9909796.1"
                     /translation="MPARVEFTGDIARLILSGDFDFSTQEDLGRAIDEILRANAIREI
                     RVDLVDATFIDSSVIRALLKLRESALEDGKSLSLWNCNDYIREIFTIGGFDQLFVMH"
     gene            complement(638..1303)
                     /locus_tag="VFQ23_24325"
     CDS             complement(638..1303)
                     /locus_tag="VFQ23_24325"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HET9909797.1"
                     /translation="MFKSRSRPIVIPQSEHLRLVGALAMLWGNEDFDSPPVERNSMVA
                     GMGLHDRGYGTLDHSAIGEMSEEEWQHIARRSFSMFGSDLTADTIIKYHIRRLATYSD
                     TDQRKALTSEFSQIIDEQLKSHALPKEVFDRMDRITELTDNISFTFCFDVPDSGEVSI
                     FPKNDSTTEITVRYQIANGTIQVEPWPFSVTRYDGYIFAYPLDGYPERLDPFILPYHL
                     VQD"
     gene            1486..2475
                     /locus_tag="VFQ23_24330"
     CDS             1486..2475
                     /locus_tag="VFQ23_24330"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET9909798.1"
                     /translation="MKNLVGFRFVYVLLIAALAFGAAPVQAAQEENTIVDVALAANAE
                     TGEFSILIAALQAANPDLLARLDGKREYTVFAPTDAAFLALLDELGVTAEQLLSDRKL
                     VSRVLRYHIARGELDSTEVLSRERIRTLQGGSLFQSGGVLTDVNGRTANIVATDIQAS
                     NGIIHVIDRVVLPRLKSGVENSIVDVAMAANDETGEFSILIAALEAANPDLLERLDRR
                     GEYTVFAPTDAAFLALLDELGVTAEQLLSDRALVSKVLRYHIVRGELDAATVLARARL
                     RTLQGGSLFQSGGVLTDVNGRTANIVATDIQASNGIIHVIDRVVLPRLNGNDD"
     gene            2646..2990
                     /locus_tag="VFQ23_24335"
     CDS             2646..2990
                     /locus_tag="VFQ23_24335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9909799.1"
                     /translation="MHLQFRELDNSVRLIKLTGRFDTTGTGEIETKFSGYCAGEKCRI
                     AVDLSDVDFLASIGIRLLLLTAKSVASRGGKMVLESPIPEVQNVLELTGIPALIPVYS
                     YLESAETILLAG"
     gene            complement(3027..4061)
                     /locus_tag="VFQ23_24340"
     CDS             complement(3027..4061)
                     /locus_tag="VFQ23_24340"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9909800.1"
                     /translation="MNYVNFGRTGVKVSQLCLGCMNFGGRQNEAESIRIIHQALDAGI
                     NYLDTANVYGHEPLHFEIGRGRSEQIVGKALGDGRRESVFLATKVHYAMGEGPNAQGN
                     SRRHIIEQCEASLTRLNTDHIDLYQLHGTDLETPIDETLRALDDLIRAGKVRYIGTSG
                     FAAWELMEALWAADVNNLHRFVSEQAPYNLLDRRIERELIPMLQTYDMALVPWAPSAG
                     GFFSEHYERGHKPPEGSRYEAFWRGLYRESFTQNQRVFDIQSVVLEMAKEKQTSGYAF
                     ALAWCMSRPGVTSPIIGPRTSDQLVDSLTAVDITLTNEDLKRLDEIAPPESVTVPYID
                     YKGKHHLRWY"
     gene            complement(4200..5909)
                     /locus_tag="VFQ23_24345"
     CDS             complement(4200..5909)
                     /locus_tag="VFQ23_24345"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9909801.1"
                     /translation="MMARFSHLLIVVAFLGTNMESGKARTSNYLHTSGNQILDASGKV
                     VGLSGVNWFGFETESAAPHGLMQRNYEEVLDQVQNLGYNVIRLPFSNAMLAPGTKPKG
                     ISYSKNPDLANLTALELMDRIIAAAKARGIRVILDNHRSTAGGGPESNGLWYTSTYPE
                     SRWIEDWKMLANRYKGNDAVIGMDLRNEPHGACWGCGNTSKDWRLAAEKAGNAILSVN
                     PELLIIVEGTANYNGQYTWWGGNLMGARDFPVRLDVSNRLVYSPHEYPESIYPQSWFK
                     ASNYPENLPGVWDKYWGYLADDYPILIGEFGSRLETEKDRQWFQKFRRYIQEKRLNWT
                     FWSLNPNSGDTGGLLLDNWSSVHQEKQNVLKSIQYPFIELTTTSLPSTSVSIKTTSTP
                     NTPRFLAGARSLENFESSWRHNWRTFKSNTSSVTSQVVSPGASGTYAMKLDYNIRKDG
                     WGGIERVFVISQDWTTYQSIQFNFYGTNSGSTIRFEVLDNRSAGSSSDTAERFEYNFT
                     DNFTGWKTFKLSWNEFVRRADWQPKGAPNDGFSLTAIRGFNFSPINGQGSFQVDDIKL
                     VNS"
     gene            6243..6746
                     /locus_tag="VFQ23_24350"
     CDS             6243..6746
                     /locus_tag="VFQ23_24350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909802.1"
                     /translation="MDERRLRKLFQFTEADLEANRRGQFSEKQRKRLLDQARAEQKSA
                     WESATILFVVAAVGLAFGLILASVAPVPFGRILFVALLCILWPSAWAGKGLQIIRSAR
                     TLQEPRLRTVSGRADIIKYDSETYVLKVGELEFDLDGNPSGVIIEGDEYTIYYVEATH
                     EILAVEF"
     gene            complement(6800..7612)
                     /locus_tag="VFQ23_24355"
     CDS             complement(6800..7612)
                     /locus_tag="VFQ23_24355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9909803.1"
                     /translation="MDENAGSTKPKDTWMSGEAYEGYIGRWSRLVAREFLKWLTVPAG
                     SQWLDVGCGTGMLSKTILEMAKPAKVRGIDRSEGFIAFAKRNVQDQRVEFEVGDAQAL
                     AVDDGTFDTTVSGLVLNFVPQPERALAEMFRVTRADGIVAVYVWDYADGMQLIRYFFD
                     SAIELDPKVAELDEGQRFSICQPDALGRLFESAGLQQVEVRPIEVPTDFRDFDDYWNP
                     FLGGQGPAPSYAMSLDEEHRAALREKIRASLPYNSDGSISLVARAWAARGVR"
     gene            complement(7654..>8784)
                     /locus_tag="VFQ23_24360"
     CDS             complement(7654..>8784)
                     /locus_tag="VFQ23_24360"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9909804.1"
                     /translation="GSPIYRDFVPTADSLVVERIKGAGAITIGKTNVPEFGAGSQTFN
                     PVFGPTRNPYDLTKTVGGSSGGAAAALASGLMPLADGSDMGGSLRNPANFCNVVGFRP
                     SPGRVPSWPVELGWFTLSVDGPMARTVSDVALLLSVLAGPDPRSPIALGDPGSVFAAP
                     LDRDFKGTRIAWLDLGLPYEPDVKSVVNSNRSVFESLGCIVEEAEPNFSDADEIFKTW
                     RAWSFEAGLSGEYQKHKDQLKDTVIWNIEQGLPLTGPQLARVEIKRTQLFHRLREFMD
                     RYEFLILPVSQVLPFDVNQPYPTEINGKKMLTYIDWMKSCYYISTVGHPALSVPGGFT
                     ASGLPVGVQVVGRHHEDWSVLQLGYAFEQATEHWKRHPDIAL"
BASE COUNT         2318 a         2256 c         2034 g         2176 t
ORIGIN      
        1 ccctctgaaa tctagtttgc gtgttgtagg cggctatatc agtgcatcac aaacaactga
       61 tcgaatcccc cgatcgtaaa gatctctcga atgtaatcgt tacagttcca cagactcaaa
      121 gatttcccgt cttccagtgc cgattcacga agcttcaaaa gagcgcgaat cacagaagag
      181 tcaataaacg tggcatcaac caagtcaact cgtatttctc tgatcgcatt ggcacgtaag
      241 atttcatcaa ttgcacggcc taaatcttcc tgtgtcgaaa aatcaaagtc ccccgataag
      301 atgaggcggg caatgtctcc ggtaaactcc actcgtgcag gcatcctggt ctccttcctg
      361 cctgattccc ttatcgcttg tcagacgaat caccaagctt ttgaaaaatt agtagagcgg
      421 tactctacta cctgaatgaa taatatatgg tgctgtttag atggacgaaa gatttgaaga
      481 caagaactgc cctattataa agaaatttca aactttggta taaaaaaata tccacccatt
      541 ggttggatta ctctggtatc ccttgcgagt tatttgcctg atctatccaa aagcttcgga
      601 attgacctcc gaagcttttt gttttacaga atcactatca gtcttgcacc aaatgatatg
      661 gcaggataaa tgggtcaagc cgctccggat aaccatccag tggataggca aagatataac
      721 catcatagcg agtaacggaa aaaggccatg gctcgacctg aatggtaccg tttgcgattt
      781 gataacgtac cgtaatttcc gttgttgagt cgtttttcgg aaagatcgaa acctcacctg
      841 agtcgggaac gtcaaagcaa aacgtgaatg aaatgttatc tgtcaactca gtgatccggt
      901 ccatcctatc aaagacttcc tttgggaggg cgtgagactt gagctgctca tcgataattt
      961 gtgaaaattc agaagtcaac gctttacgct gatcagtatc cgaataggtg gcaagccgtc
     1021 gaatgtgata cttgataatc gtatcagccg taagatctga gccaaacatc gaaaaacttc
     1081 gccgcgcaat gtgctgccac tcctcttcac tcatttctcc aatcgcagaa tgatcgagtg
     1141 tgccgtaacc gcgatcatgc aaacccatcc cggctaccat ggagtttcgt tccacaggtg
     1201 gagagtcgaa atcttcattt ccccatagca tcgccagagc tccaaccagc ctgagatgtt
     1261 ctgattgagg gataacgatc ggtctgctcc tggatttgaa catacattaa ctcccgattt
     1321 ttggcatagc atatcacaat ccctacggag ctgcattgga aacctcacac gtatcatttt
     1381 gcacagattt tattagagtt gaataataat ctgtgcaaaa cattgacacg aactggaaaa
     1441 tccttagaat gtcaagcatc agcaaaattc aataccggag gaaaaatgaa aaatcttgta
     1501 ggtttccgat ttgtgtatgt tctattgatt gctgccctgg cgttcggggc ggcgccagtg
     1561 caggcagcgc aggaagaaaa caccattgtg gatgtggctc tggctgccaa tgccgaaacc
     1621 ggtgagttct cgatattgat cgctgccttg caggctgcca atccagacct gctcgcgcga
     1681 ctcgatggca agagagagta caccgtattc gcccctactg acgcggcttt tctcgccctg
     1741 ctggatgaac tgggtgtgac ggccgaacag ctcttgagcg atcgaaaact tgtctccaga
     1801 gtgctccgat atcacatcgc ccgtggcgaa ctcgactcga cagaagtatt atccagagag
     1861 cgcatccgca ccttacaagg cggatccttg ttccagagcg gcggtgtgtt gacggatgtc
     1921 aacggtcgga ctgccaatat cgtagccact gacatccagg caagcaacgg cattatccat
     1981 gtcatcgacc gcgttgttct gcccaggctc aaatctggtg ttgagaactc cattgttgat
     2041 gtagccatgg ctgccaatga tgagaccggt gagttttcga tcctgattgc agcgcttgag
     2101 gcggccaatc cggatttgct cgaaagactc gaccggcgag gagagtacac cgtattcgct
     2161 cccaccgatg cggcgttcct ggcactgctg gatgaactgg gcgtgacagc cgaacagctc
     2221 ttgagcgatc gggctctcgt ctcgaaggta cttcgctatc acattgtccg cggcgaactt
     2281 gacgctgcga ccgttctggc aagagcgcgc ctccgcacgc tgcaaggcgg atccctgttc
     2341 cagagcggcg gcgtgttgac ggatgtcaac ggccgcacag cgaacatcgt agccaccgac
     2401 attcaggcga gcaatggcat tatccacgtc atcgaccgcg ttgttctgcc cagacttaac
     2461 ggcaacgacg attagttcgc aatccaaata ataacgtcta taaaaaacat acaccctgaa
     2521 aatccccagg gtgtatgttt tttcatcggt tgtcgcgcgc tgattcaaaa tcataacctg
     2581 ttgttaacac tgacgttttc cgaagttaat ataattagtt atacatttga ccgccggggg
     2641 aaaatatgca tcttcaattc agagaattag acaacagtgt acgattgatc aaactcaccg
     2701 ggagattcga tacgactggc accggcgaaa tcgaaacaaa attctccggc tattgtgcgg
     2761 gagaaaaatg ccgcatcgct gtggatctct ctgatgttga ttttcttgca tccataggaa
     2821 tccgcctcct tctgctaact gccaaatcag tcgctagtcg gggcgggaaa atggtccttg
     2881 agagtccaat ccctgaagta caaaatgtgt tggaattaac cgggataccc gccttaattc
     2941 ctgtctattc atatcttgag tccgctgaaa caatcttgct tgcgggctga acaatgttat
     3001 ccacttcgta accgcgaatt gcaggtttag taccaacgaa ggtggtgctt ccctttgtaa
     3061 tcaatataag gaaccgtgac cgattcaggc ggcgcaattt catccaatcg ctttagatct
     3121 tcattcgtca aagtaatatc caccgccgtt agagaatcca caagttgatc cgatgtacgt
     3181 ggtccgataa tagggctggt cactcccggg cgggacatac accacgccag agcgaaggca
     3241 tatcccgaag tttgtttctc cttggccatt tcaagcacga cgctttgaat atcaaatact
     3301 cgctggtttt gcgtaaagct ttcccgatat aagccgcgcc agaacgcttc atatcgtgaa
     3361 ccctctggcg gtttgtgccc acgctcatag tgttcggaaa aaaaacctcc ggcgctcggc
     3421 gcccagggaa ccaatgccat atcatacgtc tgcagcatgg ggatcaattc tcgctcaatg
     3481 cgccgatcga gtaagttata cggagcctgc tcgctgacaa agcgatgcag attgttcaca
     3541 tctgcggccc acaatgcttc catcagttcc caggcggcaa atccgcttgt gccgatataa
     3601 cggaccttgc ctgcgcgaat cagatcatcc agagcgcgca acgtttcatc gatcggagtt
     3661 tccaagtcgg taccatggag ctgatacaga tcaatatgat ctgtgttcag tctcgtgagt
     3721 gaggcttcac actgttcgat gatgtgcctg cgcgaattgc cctgggcgtt cggaccctct
     3781 cccatcgcat aatggacttt ggttgcaaga aatacgcttt ctcgtcttcc atcgccgagc
     3841 gctttcccaa caatttgctc acttctgccg cgcccgattt cgaaatgaag tggctcatgt
     3901 ccatagacgt tggcagtatc cagatagttg atgcctgcat ccagcgcctg atggataatt
     3961 cgaatcgact cagcttcatt ttgcctgccg ccgaaattca tacagcccag gcacagttga
     4021 ctgaccttga ctccagttcg cccgaaatta acgtagttca ttgctcaact ctcatcctct
     4081 gaattgaatt cctaataaca actgattata agattgttgt cagtcttttg cgggcgagga
     4141 aacactttag cataataaac aacaaactcc ccactttgga ttagagctgg aaggtcgatt
     4201 caggaattga caagcttgat gtcatccacc tgaaaacttc cctgtccgtt gatcggtgaa
     4261 aagttaaaac cccgaattgc tgtcaggcta aatccatcat tgggtgcgcc ttttggctgc
     4321 caatcagcgc ggcgcacaaa ttcattccag gatagtttaa aggttttcca cccggtaaag
     4381 ttatcggtaa agttgtactc gaaacgctct gccgtatcgc tactactgcc tgcactgcga
     4441 ttatccaaaa cctcgaaacg gatggtcgag ccgctattcg tgccataaaa gttgaattga
     4501 atggactgat acgtggtcca atcttgcgat atgacaaaga cacgttcaat cccaccccag
     4561 ccgtctttgc ggatgttata gtcaagtttc attgcatagg tccccgatgc acccggtgag
     4621 accacttggc tggtaacgct tgaggtattc gatttgaaag ttcgccaatt gtgtcgccag
     4681 cttgattcaa agttttccaa cgaacgagca ccagcgagaa accgcggcgt gttgggtgtc
     4741 gaggttgtct taattgagac agaagtggat ggaagagagg tcgtagttaa ctcaataaaa
     4801 ggatattgaa tagacttcaa cacattttgt ttttcctgat ggacggacga ccaattatcc
     4861 aacaacaaac caccggtatc accggaattt ggattcaacg accaaaacgt ccagttcagc
     4921 cgcttctcct gaatataacg tctgaatttt tgaaaccact gacggtcctt ctcggtttcc
     4981 agtcgtgacc cgaactctcc aattagaatc ggataatcat cagcaaggta tccccaatat
     5041 ttatcccaaa cacctggcag gttttcagga taatttgagg ctttgaacca ggactgcgga
     5101 taaatggatt cagggtattc atggggtgaa taaaccaaac gatttgaaac atctaaccga
     5161 acaggaaaat ccctggcgcc catgagatta ccgccccacc aggtatactg cccgttgtaa
     5221 ttagcagtcc cttctacaat gatcaatagt tccgggttga ccgagagaat ggcgttcccc
     5281 gccttttcag cagccagcct ccaatctttg gatgtattgc cacagcccca acaagcgcca
     5341 tggggttcat tgcgcagatc cataccaatc acggcatcgt tacccttata acgattagca
     5401 agcatcttcc aatcttcgat ccagcggctt tccgggtaag tactggtgta ccaaagcccg
     5461 ttcgattcgg gtccaccacc agcagtagaa cgatggttat ccaggatcac gcggattccc
     5521 cgcgctttcg cggcagcgat gattcgatcc atcaactcga gtgcagttaa gttagccaga
     5581 tctggatttt ttgaataact tatccccttc ggctttgtgc caggagccag catggcattt
     5641 gagaaaggca atcgaatcac gttgtatccc aaattctgaa cctgatccag cacttcctcg
     5701 taattacgct gcatcaaccc gtgcggtgcc gccgactcag tttcaaagcc aaaccagttc
     5761 acaccactca agccgacgac cttgcccgat gcgtcaagaa tctgattgcc cgaggtatga
     5821 agataattgg aagtcctagc cttgcctgat tccatatttg ttccgagaaa ggcaacgaca
     5881 atcaatagat ggctgaatcg agccatcatg gctaagatgc ttttgaaaat tctattaagg
     5941 gatgcgctta acattagtat agttgagtta ccggtggaat acgagaacag tttcaggcta
     6001 ggcacagcga gtaaaagtga atgataatct tcatatgccc acacctggta tgtttatagc
     6061 gaatgacaat gaatttcaga ttaaaagcga cagcgctggg tataggacat ttgtaacgta
     6121 accgaacagg atatctccac ttgttggaag aacggcaaaa cttacagaaa tatgctcaat
     6181 ttacacatta aaatttgtac agatacacgt tagaccattg aagattaaag gcagtgagaa
     6241 ccatggatga gcgacgcctg agaaagttgt ttcaattcac cgaagctgat ctggaggcaa
     6301 atcgccgcgg tcagttttcc gagaagcaaa ggaaacggtt actggaccag gcaagggcag
     6361 aacaaaaatc cgcctgggaa tcagcaacga ttttatttgt agtggctgct gtgggtttgg
     6421 ctttcggact gatccttgct tctgttgccc ctgtaccgtt tggtcgtatt ttgtttgtag
     6481 ccctgctatg catcctgtgg ccttctgcct gggccggcaa aggattacag atcattcgat
     6541 cagcgcgcac tttacaggag ccgcgcctgc ggacggtgag cgggagagcc gacatcatca
     6601 aatacgacag tgagacttat gttttgaaag tgggagaact ggaattcgac ctggatggga
     6661 atccatctgg cgtgatcatc gaaggggacg aatatacgat ctattatgta gaggcaacgc
     6721 acgagatact tgcagtagag ttttagatac gctacgaaag cgaaaaatcc ctctcgatga
     6781 gacggtttcc ctggacgatt taacgcacac cacgtgccgc ccatgcccga gcgaccagtg
     6841 aaattgaacc atctgagtta taaggcaggc tcgctcgaat cttctcacgc aacgcagcgc
     6901 ggtgttcttc atccaaagac atcgcataac ttggagcggg accctgtcct ccaagaaacg
     6961 gattccaata atcgtcaaaa tcgcggaagt ctgtgggaac ttcaatcggg cggacttcta
     7021 cctgttgcag gcccgccgat tcaaaaagcc gacccagtgc atcaggttgg cagatcgaaa
     7081 aacgctggcc ttcatcaagc tcagcaactt tgggatccaa ttcaatggca gaatcgaaga
     7141 aataacggat taattgcatt ccgtctgcgt aatcccagac atatactgcg acaattccat
     7201 cggctcttgt cacacgaaac atttctgcaa gtgcgcgctc cggttgtgga acgaagttaa
     7261 gaacaagacc agatacggtt gtatcgaagg tcccatcatc gaccgccaac gcctgcgcat
     7321 ccccgacctc aaactcaacc ctttgatctt gcacatttcg ctttgcaaag gcaataaatc
     7381 cttccgagcg gtcaatacct cggactttgg ccggcttcgc catttcaaga atggttttgc
     7441 taagcatacc tgttccgcag cccacgtcca gccactgact gccagctgga actgtgagcc
     7501 atttcaaaaa ctcacgtgca accaggcggc tccaacgacc gatatatcct tcatacgcct
     7561 ccccactcat ccatgtgtct tttggttttg ttgaaccggc gttctcgtcc atgagatgct
     7621 cctaatagat ctgtcttgga caatcaacca ccatcaaagt gcgatgtcag gatgacgttt
     7681 ccagtgctca gtggcttgct caaaggcata acccagctgc aatacactcc aatcctcatg
     7741 atggcgtcca accacttgta cgccaaccgg caacccagag gcagtaaacc ctcccggcac
     7801 cgataatgcc ggatgcccta ccgtcgaaat gtaataacag gacttcatcc aatcgatata
     7861 agtgagcatc ttcttgccgt ttatttcggt tggatatggc tgattcacgt caaatggtaa
     7921 aacctggctt actggcaaaa tcaggaattc ataacgatcc ataaactcgc gtaatcggtg
     7981 aaataactgc gttcgcttga tttcgactcg ggcgagctgc ggaccagtca gtggcaagcc
     8041 ctgttcgatg ttccagatca ccgtatcttt taattgatct ttatgctttt ggtattcgcc
     8101 agacagacca gcttcaaagc tccaggcgcg ccatgtctta aagatttcat ccgcatctga
     8161 gaaattgggt tcggcttctt caacaatgca gcccagcgac tcaaatacgg atcggtttga
     8221 attcaccacg cttttcacgt caggttcata cggcaaaccc aaatctaacc aggcgatacg
     8281 cgtccctttg aaatcgcggt caagaggtgc ggcaaacacg gagccgggat cgcccaaagc
     8341 aattggcgaa cgaggatcgg gtcccgcgag aactgaaagg agtaaggcaa cgtctgagac
     8401 ggtacgcgcc atcggtccgt ctacagacaa ggtaaaccaa cccaattcaa ccggccacga
     8461 gggaacgcgc ccgggcgagg gtctgaaccc caccacatta caaaaattgg ccgggttgcg
     8521 aagcgagccg cccatatcac ttccatcggc aaggggcatg agtcctgaag ccaacgcggc
     8581 tgccgcgcca ccgctgctac caccaaccgt cttggtgaga tcatatggat ttcgcgtggg
     8641 gccgaaaact ggattaaacg tctgtgaccc cgcaccgaac tcaggcacgt ttgttttgcc
     8701 gatcgtgatc gcacccgcgc ctttaattcg ctcgactacc agcgagtctg cagtgggaac
     8761 aaaatcacga taaatcggcg aacc
//
LOCUS       DASTJP010000835         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_925318, whole genome shotgun sequence.
ACCESSION   DASTJP010000835 DASTJP010000000
VERSION     DASTJP010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925318"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VFQ23_24365"
     CDS             complement(<1..694)
                     /locus_tag="VFQ23_24365"
                     /inference="COORDINATES: protein motif:HMM:NF014437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET9909805.1"
                     /translation="MRERLQQFGGFLAGMVIPNIGAFIAWGLITALFIPTGWIPNENF
                     AKLVGPMILYLLPVLIGYTGGKMIHGHRGGVVGAAATMGVVVGTDIPMFLGAMIMGPL
                     GGWLMQKVDTYLEPKVGVGFEMLVNNFSAGILGLILALFGFLVVGPVVTVFSNAAGGV
                     VDFLVNARLLPLAAIIIEPAKVLFLNNALNHGVLAPLGVAAVNESGRAIHFLLETNPG
                     PGLGLLLAFYVAG"
     gene            1194..2180
                     /locus_tag="VFQ23_24370"
     CDS             1194..2180
                     /locus_tag="VFQ23_24370"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9909806.1"
                     /translation="MASIKDVAEAAGVSTATVSRVLANKSRVHAETRKRVSAAIERLN
                     YRPNLIARGLRAQKTAKIGLVVSDIRNPFFTAISRAVEDAAYERGYSVLLCNTDENPE
                     KEEMYINLLHDENVAGIIFSPTQKFGASRKSYMSTTPFVAIDRYIENKNMDMVLLDNV
                     SAGYELTLHLIENGYSNFAGLFGNASTTAPERRRGFQMALDDNKLTPVEIRVLEPRIK
                     QGYETTMSLLAADKKPDAIFTSNSLLTAGAFQAIRDSKLSVPGDVALVGFDETTWGAL
                     VDPPITIIAQPTEEIGLTATELLFQRISEPERSPKTVILKGKLLARGSSAPR"
     gene            complement(2196..>2432)
                     /locus_tag="VFQ23_24375"
     CDS             complement(2196..>2432)
                     /locus_tag="VFQ23_24375"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET9909807.1"
                     /translation="TTKQANEKIMNFLLEMGPLERLAIMHTGAESRAKEFLNAVMQQA
                     SQSVPRDILIVNVTTVIGTHIGPNGLGFAAVKKS"
BASE COUNT          663 a          614 c          567 g          588 t
ORIGIN      
        1 taccagccac atagaaagcc agcaacaagc caagtccggg tcccgggttg gtctcgagca
       61 ggaagtggat ggcacgacca ctttcattca cagctgccac acccaacggc gccagcacac
      121 cgtggttcaa ggcattattg aggaagagta ccttggcagg ttcgatgatg atcgccgcca
      181 gcggcagaag ccgggcattc accaggaaat ccaccacccc tccagcagca ttgctaaaga
      241 ccgttaccac tggaccaacg acaaggaacc caaacagggc caggatgagt cccagaatgc
      301 cagccgagaa gttgttgacc agcatctcga agccaacacc aaccttgggc tcgagatagg
      361 tgtccacttt ctgcatcaac caaccgccca gaggtcccat aatcatggca ccgaggaaca
      421 tagggatgtc agttccgaca acgacaccca tcgttgcagc cgcgccgacg acgccaccgc
      481 gatgtccatg gatcatcttg ccaccggtat aaccaatcaa gacgggaagc aggtacagga
      541 tcattggacc aaccagcttc gcaaaatttt catttggaat ccagccggtc gggataaaca
      601 gggctgtgat caaaccccag gcaataaacg cgccgatatt agggatgacc attccagcca
      661 aaaaaccacc gaattgttgc aatcgctctc tcatcttttc tctccttgat tttgaaaatt
      721 cgcctgagtc tcacaggctt ttgcttgcac aggtaacagt cggagtgtta ccaggtgacc
      781 aaaaagaaag attggaaatg tttgccaaaa agatatgtat aaaaatgaaa aatttcgttc
      841 cgcagtggaa aaaggacgtt gaccccattg attcctttcc gccttcgaat tgaccgaagt
      901 atctgtttca gcttgctgta ttacagtggt ttccagcatt tgacctcatg atcaaactta
      961 tgatggttga agcagctttg gagccattcg gtgggaaaat gttggctcgg agtgtaaacg
     1021 attacacaaa ctttgtcaat ggattaatct ggtactcata ataataggtc gcttggcttg
     1081 aaaagacctg tttttgtatt aaatgtgctc ttacggaccg ttttccgata cttataaccc
     1141 tgatggtatg aaatcgtttt caccaattta aagaaaatgg tatacttctc agcatggcaa
     1201 gcatcaaaga cgtagccgaa gccgccgggg tatccacagc cactgtctct cgggtgttag
     1261 caaataaatc tcgtgtgcac gctgaaacca gaaagcgcgt ctcagcagct atcgaacgtc
     1321 tcaactatcg gccgaatctg attgctcgag gcttgcgggc tcagaaaaca gccaagattg
     1381 ggctcgtcgt ttccgacatc cgtaatccgt tttttactgc gatcagccgt gcagtggaag
     1441 atgcagccta tgagcggggt tattctgtat tattgtgcaa taccgatgaa aaccccgaaa
     1501 aagaagaaat gtatatcaac ctgctgcatg atgaaaacgt cgcaggcatt atcttttctc
     1561 ccactcaaaa atttggtgca agccgaaaat cctacatgtc caccaccccc tttgtagcca
     1621 ttgatcgtta tatcgaaaac aagaacatgg atatggttct tttagacaac gtatccgctg
     1681 gatatgagtt gacccttcac ctgatcgaaa atggctatag caactttgca ggtctcttcg
     1741 ggaatgccag cacgaccgca cccgaacgaa ggcgcgggtt tcaaatggca ctggatgaca
     1801 acaagttaac tccggtagaa atacgtgtgc ttgaaccgcg catcaaacag ggctatgaaa
     1861 caaccatgtc attattagcg gccgacaaaa agcctgacgc catatttaca agcaacagcc
     1921 ttcttacggc tggcgcgttt caagcgattc gggatagtaa attatcggtg ccgggtgatg
     1981 ttgccctggt gggattcgac gaaacaacct ggggtgcgtt ggtggaccct ccgattacga
     2041 tcattgcgca gccgacggag gagatcgggc tcacagcgac agaattgctt tttcaacgga
     2101 tctctgaacc ggaacgttcc cccaagactg ttattctcaa agggaaactt ttagcgcgtg
     2161 gttccagtgc gccccgctag atgcatagcg ttagtctaag attttttcac tgcggcaaac
     2221 cccaaaccat tgggtccaat atgggttccg atcacggtcg tcacattgac gatcaaaata
     2281 tcgcgcggca cagactggct tgcttgttgc atcaccgcat ttaggaattc ctttgcacgt
     2341 gattcagcgc ccgtatgcat gatcgccaat ctttccagtg gacccatttc caataaaaag
     2401 ttcataattt tttcgttggc ttgtttcgtt gt
//
LOCUS       DASTJP010000836         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_608544, whole genome shotgun sequence.
ACCESSION   DASTJP010000836 DASTJP010000000
VERSION     DASTJP010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608544"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFQ23_24380"
     CDS             <1..269
                     /locus_tag="VFQ23_24380"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9909808.1"
                     /translation="RFIYFYFNRNEPEKIHQVVPAHVEYWRSLNMEGYMGGPFTDRTG
                     GLISFLDSNLEEAEEIMRQDPFVSADLIEQKWIKQWIVESHAAA"
     gene            431..1366
                     /locus_tag="VFQ23_24385"
     CDS             431..1366
                     /locus_tag="VFQ23_24385"
                     /inference="COORDINATES: protein motif:HMM:NF016124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909809.1"
                     /translation="MNGSKNKKAEESLTRAMRRVEGFRDWLVAGRFRNHANSTTTSWP
                     VVPGAYVVGDRLGPVAICTLTSNALFAPLATLPEVAIAGRAYSANLGIEKMISNVSAN
                     PSIRFLLLCGKESPYFQVGQALDALFAYGVTSGGRIIKASGHMPELSRVEMTHIEHFR
                     KQVELVNCIGETNLDRLAAVVKELSGRSPGLCQDTPLEPSVVASGRKLGDGFRPIVLG
                     GHRESLAYDPMGFFIITLDREVNEILVHHYWSDHSPAHLVRGRNGEAILLALLREEII
                     SERSHAGYLGAELAKAETAIRLGLQYEQDQPLRLQ"
     gene            1636..2496
                     /locus_tag="VFQ23_24390"
     CDS             1636..2496
                     /locus_tag="VFQ23_24390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9909810.1"
                     /translation="MIRQHPLSFFFGLTFLLSWLIWVPMALDHYSLLPFELDENFVLI
                     VRLFGTLGPAIAASLVALIVGGRSAVRKLWSQIGLWRVSWTWYVAAALVFPVLVFVAA
                     WLYNLLPGIEPLLIQQISPATLLVIMIIMTISVTGEEIGWRGFALPEMQKRWTALKTS
                     LLLGTIHTLWHLPFWITLGELERYGWSYWLLSWAWILALTIYMTWILNNTGNSLLMIL
                     LFHFSLNVVSNAFLPVTTVVPAYLLLIGIAWLLALSILARYGPQRLVRSNSGTPGIPP
                     ELIRSSGLSD"
     gene            2584..3441
                     /locus_tag="VFQ23_24395"
     CDS             2584..3441
                     /locus_tag="VFQ23_24395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9909811.1"
                     /translation="MFTSFGRWIKAHDLLTYFVLAFLFTYAAWSVTLFTAGADDPNTM
                     IDLPTVFRSLGMWGPAAAGIVVMAVTRGKAGLQEMFRRVFLWRVPLRWYLLILFIWPV
                     IVILSDFLYAQITNQSIRFEWNGWRQTTIWLAQSPFIAFWASEEIGWRGFALPRLLSR
                     WNALVSSLIVGTIHALWHLPLFFMGNPPLGYWLFTVSLAIFMTWLMNNNRGSVFLAIF
                     FHFWVNVYGGIHADRFFIANPDPAQRMIIQGFMMAAFAIVVVILYGYRTLTRERENPM
                     LAELKPSAS"
BASE COUNT          752 a          873 c          893 g          933 t
ORIGIN      
        1 agcgctttat ctatttttac ttcaatcgaa atgaaccaga gaagatccat caggttgtcc
       61 ccgcccatgt ggagtactgg aggtcgctga acatggaagg ctatatgggc ggtcctttca
      121 ccgataggac aggcggcttg atttcttttc tggactcaaa cctggaagag gcggaagaga
      181 tcatgcgaca agatccattt gtttctgctg atttgatcga gcagaaatgg atcaaacagt
      241 ggattgttga atctcatgca gcagcatagc atagagcgtt tataaaatct aatcaaaagc
      301 atgaatgcca tactctgcag attgatcagt ttgctcctcg caaatggtac ttcactacct
      361 gattaagggt agaggtcagg aatactgcgc tgcaaaatga cattttgtga agcacttccg
      421 gagataaaca atgaacggtt ccaagaataa aaaagctgaa gagagcctta cgcgcgccat
      481 gcgcagggtg gaaggtttca gagattggct tgttgcgggc cgatttcgga atcacgcaaa
      541 ttcgacgaca acctcctggc cagttgttcc gggggcctat gtagtcgggg ataggcttgg
      601 ccctgtggct atctgtacat tgactagcaa tgcgctgttc gcgcctctgg ccacactgcc
      661 ggaggtagct attgccgggc gggcatactc agccaacctg ggcattgaga agatgatttc
      721 taacgtatct gccaatccat caatccgctt tctgttgctt tgtggaaaag agtctcccta
      781 cttccaggtt ggtcaggctt tagacgcctt gtttgcctat ggggttactt caggaggaag
      841 gattattaag gctagcggcc acatgcctga actaagccgt gttgagatga cacacattga
      901 gcatttccga aaacaagtcg agttggtgaa ctgtatagga gagaccaatc ttgacagact
      961 ggctgctgtc gtaaaggaac tctccggccg gagccccgga ctctgccaag atacccccct
     1021 tgaaccgtca gtcgtggcgt ccggacgtaa acttggcgat ggctttaggc cgattgtgct
     1081 gggcgggcat cgcgaatcgc tcgcctacga tccgatggga tttttcatta tcacacttga
     1141 tcgggaggta aatgagattc tcgttcacca ctattggtcc gatcattccc ccgctcacct
     1201 agtgagaggc cgaaatggag aagcgatttt gcttgcgctg ctgcgcgaag agattatctc
     1261 cgagagaagc catgctggtt accttggcgc ggagctggca aaagccgaaa ccgcgattcg
     1321 tctgggcctg caatatgagc aggatcagcc actgcgatta cagtaatggc tttatgggag
     1381 ctttccaatg tcaataacgt cgcgccgaat caatgagcga ttacatatct cctacttttg
     1441 gatgacgatt ggtctttttt gtctcttggc taagctatag aatgttatat aaagatgtct
     1501 actcaagtca acagaaggga acaacattca gagtgatttt gttttcttaa tttccatacc
     1561 gaagccctga cgtctgcgta tcttatatgc ctcgccgaca tacttaaatt ctgatcgaat
     1621 gggaaaatca aaaccatgat tcgtcaacat cctttgtcat tcttcttcgg ccttacattc
     1681 ctactctcct ggttgatctg ggtgccgatg gcgctggacc attactcgct gctcccgttc
     1741 gaactcgacg agaatttcgt actgatcgtg cgcctgttcg ggaccctcgg cccggccatc
     1801 gccgcttccc tggttgccct catagtaggc ggtagatccg ctgtcagaaa attatggagc
     1861 caaatcggtc tgtggcgggt cagctggacc tggtatgttg ccgcagcgct tgtgtttccg
     1921 gttctcgtat tcgtagccgc ctggctgtat aaccttttgc ccggcatcga accactcctc
     1981 atccagcaga tctcgccggc gacattgctg gtcatcatga tcatcatgac catcagcgtt
     2041 accggggagg aaattggctg gcgcgggttt gcgctgcccg aaatgcagaa acgctggaca
     2101 gccttgaaga ccagcctgct gctgggcacg atccataccc tctggcattt gccgttctgg
     2161 atcacactgg gggaattaga acgctatggc tggagctact ggctcctgag ttgggcctgg
     2221 atcctggcgc tcaccattta catgacctgg atcctgaata acacaggcaa cagcctgttg
     2281 atgatactgc tcttccactt ctctctgaat gtagtgagca atgctttcct gccggtcacc
     2341 acagttgtac ccgcttacct cctgttgatc gggattgcct ggcttctggc actcagtatc
     2401 ctggctcgct atggtccaca acggttggta aggtccaact ccggcacacc aggtattcct
     2461 ccagaattaa ttagatctag cggactttca gactaaagac gaagaactgt atctatcgga
     2521 atacaggctg actttggatg attttgacgg gccgggcctg ccaaaatcac ggcggaggtt
     2581 cagatgttta cttcgttcgg tcgttggatc aaagctcacg atttgttgac ctattttgtt
     2641 ttggctttcc tgttcaccta tgccgcgtgg tcagtcactc tctttaccgc gggagcagat
     2701 gatccaaaca ctatgataga tctcccaacg gtcttccgat ctctcggaat gtggggaccc
     2761 gcggcagcag gaatcgtggt catggcggtt acccgcggca aagcgggctt gcaggagatg
     2821 ttccgccgcg tgttcttgtg gcgggttcct ctccgctggt atttgttgat ccttttcatc
     2881 tggccggtca tcgtgatcct gagtgatttc ctgtatgctc agatcacaaa ccagtctatt
     2941 cgttttgaat ggaatggctg gaggcagacc acgatatggc tggctcagtc tcctttcatt
     3001 gcgttttggg cctctgagga aatcggctgg cgcgggtttg ccttgccgcg cctgctgagc
     3061 cgctggaatg ccctcgtctc cagcctcatt gtgggcacga tacatgcatt gtggcacctg
     3121 ccattattct tcatgggcaa ccctccgctg ggttactggc tctttacggt ttcgcttgcc
     3181 attttcatga cctggctcat gaacaataac cggggcagtg tgtttcttgc cattttcttc
     3241 cacttctggg tcaatgttta tggtggcatc cacgcggaca ggttttttat cgctaaccct
     3301 gatcctgctc aacggatgat catccagggc tttatgatgg ccgcgtttgc aatcgtggta
     3361 gtgatcctgt acggttaccg gaccctcacc cgcgagcggg agaacccgat gcttgccgag
     3421 ctgaaaccaa gtgcttcgtg atccaaaata t
//
LOCUS       DASTJP010000837         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_447972, whole genome shotgun sequence.
ACCESSION   DASTJP010000837 DASTJP010000000
VERSION     DASTJP010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447972"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1109)
                     /locus_tag="VFQ23_24400"
     CDS             complement(123..1109)
                     /locus_tag="VFQ23_24400"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9909812.1"
                     /translation="MSTVLIVDDEYSGRQTLESVLDRDGYHIEMAENGLQALEKVKAL
                     QPDVVLLDVMMPGMTGFEVCQRIRSDPLIAEIPIIVLTALDDRESLLNALKAGADDFV
                     TKPFDRFELRARLIGITKLNRFHKLVQERAKLREAHAQLLKAYEATIEGWSHALELRD
                     RETEGHSQRVTEMTVELARAYGMSEDELIQIRRGSYLHDIGKLGVPDQILHKPGALTD
                     EEWKVMKKHPQFACDMLYPIEYLRPALDIPRCHHERWDGSGYPHGLRGEAIPISARLF
                     AVVDVWDALTSDRPYRKAWTKEKALAYIIEQSGKYFDPEVVELFSKNVKIGG"
     gene            complement(1210..1860)
                     /locus_tag="VFQ23_24405"
     CDS             complement(1210..1860)
                     /locus_tag="VFQ23_24405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909813.1"
                     /translation="MIRVIVAEDHLMVRAGIRALLEKAGDVHVIAEASNGQEAMEMTQ
                     EQKPDVLIMDIMMPRMNGIQAAKQIRDMKLPTKILILSMYADEGFVHQALQCGVKGFV
                     LKSSVSDELIQAVRAIANRQTFLSGPISEIIIESAMNPRIAQSGDPLSVLSPREKEVL
                     QLIAEEHTSGEIAKLLFVSEKTVEKHRARLMEKLNVRNIAGLVRMAIKFRLVDRDI"
     gene            complement(1857..>2036)
                     /locus_tag="VFQ23_24410"
     CDS             complement(1857..>2036)
                     /locus_tag="VFQ23_24410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909814.1"
                     /translation="GRGFVEGETQSNGIGLTGLRERVTLAGGKFSISSSPVRGTIVSA
                     QFQMPEVYGNEEGQA"
BASE COUNT          490 a          565 c          451 g          530 t
ORIGIN      
        1 tccctaggca ccaaaatata aaaacccgct tattggcggg tctttgattt ttggtcggga
       61 aaatgttttg tgcagttctt ttgcttcaat tacaggataa tcacgatgaa caacaacgag
      121 ccttacccac cgattttcac attctttgaa aaaagctcta ctacctctgg gtcaaaatac
      181 ttgcctgatt gctcgatgat gtaggcaaga gccttttctt ttgtccatgc cttacgataa
      241 gggcgatcag acgtcaacgc atcccataca tccaccaccg cgaacaaacg cgctgaaatt
      301 ggaatcgcct cgcctcgtaa accatgtgga tacccgctcc cgtcccatct ctcatgatgg
      361 cagcgaggga tatcgagcgc aggtctcaga tattcaatgg gataaagcat gtcgcatgcg
      421 aattgagggt gcttcttcat caccttccac tcttcgtccg tgagcgcgcc aggtttatgc
      481 aaaatctgat caggaactcc gagttttccg atatcatgaa ggtacgaccc tcgtcggatc
      541 tggatcagtt catcttcgct catcccatac gcccttgcca actcgactgt catctctgtg
      601 acacgctgac tatgcccctc ggtttcccga tcacgcaatt ccaatgcatg agaccagccc
      661 tctatggttg cttcgtacgc cttgagcagt tgagcgtgcg cttcccggag cttggcgcgc
      721 tcctgcacaa gcttatgaaa gcggtttagt tttgtaatac cgatcaatcg cgccctcaac
      781 tcaaaacggt cgaaaggctt ggttacaaaa tcatcggcgc cagcctttaa tgcattcaac
      841 aatgattcgc ggtcatccag ggctgtgaga acgataatcg gaatttcggc gatcagaggg
      901 tcactacgaa ttctttggca gacctcaaag ccggtcatcc caggcatcat cacgtccaga
      961 agaacgacgt caggctggag cgcctttact ttttccaacg cctgtaaacc gttttcagcc
     1021 atttcgatat ggtatccatc ccgatccaaa acggattcaa gcgtctggcg ccccgagtat
     1081 tcgtcatcga caatcaagac agtgctcatg ttactctcct ggcaaatcgg aaaaactgat
     1141 ttcaggcaat catattcgtt aaaatccacc tgcacatgtg ggtaaacacc catttagcga
     1201 aggaaaattc tagatatcgc gatcgacaag gcgaaactta attgccatac gcaccagtcc
     1261 agcgatattg cgaacgttga gcttttccat gagcctggca cgatgtttct caacggtttt
     1321 ctcactcacg aatagaagct tggcaatttc accgctggta tgttcttccg cgatcaactg
     1381 caggacttct ttctcgcgcg gcgaaagaac cgacaagggg tcgcctgact gggcaatacg
     1441 gggattcatc gcactctcaa tgatgatctc cgagatcggg ccactgagaa acgtctgtcg
     1501 attggcaatt gcgcgtacgg cctgaatgag ttcatcactg acagaggact ttagaacaaa
     1561 tcctttcact ccacattgca atgcctgatg cacgaatccc tcatcagcat acatggacag
     1621 gatcaggatt tttgtcggaa gcttcatatc tcgaatctgt tttgcggcct ggatgccgtt
     1681 catgcgcggc atcataatat ccatgattaa gacatcaggt ttttgttcct gtgtcatttc
     1741 catggcttcc tgcccattgg aagcctcggc gattacatgc acatcacccg ctttttccag
     1801 gagcgcgcgg attcctgccc gaaccatcag atggtcttct gcaacgatga cgcgtatcat
     1861 gcttggccct cttcatttcc ataaacttct ggcatctgaa actgtgccga cacgatggtg
     1921 ccccgaaccg gactggaact gatgctaaac tttcctccgg cgagcgtcac tctctcgcgc
     1981 aggcctgtca acccgattcc gttggattgc gtttctccct ccacgaagcc gcggcc
//
LOCUS       DASTJP010000838         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_503001, whole genome shotgun sequence.
ACCESSION   DASTJP010000838 DASTJP010000000
VERSION     DASTJP010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503001"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="VFQ23_24415"
     CDS             complement(<1..1769)
                     /locus_tag="VFQ23_24415"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9909815.1"
                     /translation="MKTLILGFDSFDPAIFEEMAGQNQLPNLEKFISHGGYSRLEVCS
                     PPQTEVSWTSIATGVDPGGHGIFDFVHRDPQTYLPYVSILPTRKGALGEQFVPPYKAK
                     TFFEEAAQLGYPATALWWPAMFPARPELPVNTLPGLGTPDIRGQLGVGTLFSTEVEVK
                     QKTRVLKLELAGKDRYQAELPGPQVPDKSGPRFVTVPLTIDVLDEKTAKLTIGGHNIQ
                     LQVGEWSQILELQFKVSLLFTVHAITRVILTNLKSGIRLYFLPLQIHPLHSTWHYASS
                     GSFAKRIWKNTGPFLTLGWPQDTTGLEEGCITDEQFLTLCREIFDQRTKILYHLLGDF
                     NEGVLASIFDCLDRVQHMFYHDRQDIVREWYQRLDHFVGDVQEKIGSWNSKHRFLVLS
                     DHGFSEFRNKVHLNRWLLDHGYLNFINGNSSGDLRDVDWEKTRAYSVGLNSIYLNIHG
                     REGNGSVPADAIESTLAEIKDNLLDWKDVDGSAVIKHARLKHEVYSGPYTRFGPDLVV
                     GYASGYRSSAETGLGKIPEVAMEPNHDHWGADHCMDADVVPGVIFANRELRDFGKISF
                     RDIPFLAIGKHLDQSHLKPPSQMT"
     gene            complement(1782..1943)
                     /locus_tag="VFQ23_24420"
     CDS             complement(1782..1943)
                     /locus_tag="VFQ23_24420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET9909816.1"
                     /translation="MYIDPNTGGVLFQALAGALVAISGVLFVFSRRIKEGIARLRRKA
                     RKDEEDRGQ"
     gene            complement(2133..>4044)
                     /locus_tag="VFQ23_24425"
     CDS             complement(2133..>4044)
                     /locus_tag="VFQ23_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET9909817.1"
                     /translation="KEKMPAAYKQFMTISSRLEKHYKDLQDVEFTIERGKLWMLQTRS
                     GKRTAKSAVKVAVDMANEGLITKEQAVLRVTPDNVDSLLHPQFDETAMNEAEKTGTFL
                     AKGVNASPGAAVGQVYFDADTAERMAKEEKQDTLMVRPFTKPDDVHGMIASKGVLTSE
                     GGATSHAAVVARQFGIPCVVGASAIKIDLERRQMTVGDAVVKEGEWISVDGTTGKVFL
                     GKIPTSAPSLDEQTDLLTLLKWADDICARKDIRAASAKGYPTRGLQVWANADYPKDAQ
                     RARSYGAVGIGLCRTEHMFFEPERLPIVQRMILSGSSEGRTVALSELLPFQRKDFDGL
                     FEAMDGYPVIIRLIDPPLHEFMPDEEKLLEEVITLRVKNESDGLKEKEDLLSAIKGLH
                     ESNPMMGLRGVRLSIAMPEIVEMQVRAIFEAAADCTKRGIVVKPEVMIPLTGTVKELE
                     WIQPRLERIAGTVMQEKKVKFEYKFGTMIEIPRAAVTAEEVAQRAEFFSFGTNDLTQM
                     TYGYSRDDAERNFLITYQEQGILEKNPFQTLDRTGVGKLMEWAIAEGRKTRPNLEVGI
                     CGEHGGDPSSIEWCHIIGNNYVSCSPFRVPIARLAAAHSALKYRPQKAATAKKKSPAK
                     SKTVKKTAKSKK"
BASE COUNT          977 a         1038 c          980 g         1049 t
ORIGIN      
        1 gtcatttgtg agggtggttt gaggtgcgac tgatccaggt gtttgccaat tgccaaaaag
       61 ggaatatcac gaaaggagat cttgccgaaa tcacgcagct cacgatttgc aaaaatcacg
      121 cctggcacca catctgcgtc catgcaatga tcggctcccc agtggtcgtg attgggttcc
      181 atggcaactt cggggatttt gccaagaccg gtctcagcgg atgaacggta cccggaagcg
      241 tagcctacca ccaggtccgg cccgaagcgg gtgtagggac ctgaatacac ttcatgtttc
      301 agacgcgcat gtttgatcac cgcgcttccg tctacgtctt tccaatccaa taaattatct
      361 ttgatctcgg caagtgtaga ttcgattgcg tcagcaggga cgctgccatt tccttcccgt
      421 ccgtgaatgt tgagatagat gctgttcaag ccgactgaat aggctcgagt cttttcccaa
      481 tcgacatccc ttaaatcacc tgaagagttt ccattaataa agtttagata accatgatcc
      541 aataaccagc gattcaaatg gactttgttt ctgaactcgc taaagccatg atccgataat
      601 acaagaaaac gatgtttact attccatgaa ccgatcttct cctgaacgtc gccaacgaaa
      661 tgatcaagac gttgatacca ttctcggaca atatcctgac ggtcatgata aaacatgtgt
      721 tgcacgcggt ccaagcagtc aaaaatgctc gccagcacac cttcgttgaa atcacctaat
      781 aagtgataaa gaatcttcgt tctctgatca aagatctccc ggcataacgt cagaaactgt
      841 tcatcagtta tgcagccttc ctccaaaccg gttgtatcct gtggccagcc gagtgtgagg
      901 aaaggcccag tattcttcca tattctttta gcaaacgaac cggaagaagc gtagtgccat
      961 gtggaatgca aagggtggat ttgcaaaggc aggaaatata aacgaatgcc actcttgagg
     1021 tttgttaata tcactcgtgt aattgcatga accgtgaaaa gtaaacttac cttgaactgt
     1081 aactcaagga tttggctcca ttccccaact tgcagttgaa tattgtgtcc gccaattgtt
     1141 agtttcgcgg tcttttcatc cagcacatcg atagtaagcg gtacagtaac aaagcgcgga
     1201 ccagacttat caggaacctg aggacccggt aactcagcct gataacgatc ctttcccgca
     1261 agttccaatt taagcacccg agtcttttgc ttcacttcta cttctgttga aaacagagtg
     1321 ccaacaccca attgaccgcg aatatctgga gttcctaatc cgggcagagt attcaccggc
     1381 agttcggggc gcgccgggaa catagcgggc caccacaagg cggtggctgg gtaacctaac
     1441 tgggccgcct cttcaaaaaa tgtcttcgct ttgtagggtg ggacgaactg ttcgcccaac
     1501 gctcctttgc gggtaggcaa aatggaaaca tacggcagat aggtttgagg gtcgcggtgc
     1561 acaaagtcga agatgccgtg accgcccgga tcaacacccg tggcaatgct cgtccatgac
     1621 acttcagttt gcgggggaga acatacctcc agccgggaat agccgccgtg cgagatgaac
     1681 ttttccagat ttggtaattg gttttgtccg gccatctctt caaatatggc cggatcaaat
     1741 gagtcaaacc ccaggataag cgttttcatg aaaccgcgaa gttattgccc gcgatcctct
     1801 tcatctttac gtgctttgcg gcgcaggcgt gcaatgccct ctttaatcct tctggaaaag
     1861 acgaataata cgccagaaat tgctactaat gccccggcga gcgcctgaaa caagacgcca
     1921 ccggtgttgg gatcaatata cataggtact ccttacagca cgcagtcgtt cgtaagttgg
     1981 aaatgttaat ggggattttt gagcgactac gtcgtggaat ttttgttttc tatgataatt
     2041 ttatcaccat aaaattagtt caaaatgtgt gagtgaataa accaacgata aaaaacctgc
     2101 cccaaagaaa tttctttggg gcagataagg ttttacttct ttgactttgc ggttttcttc
     2161 acagttttgc tcttggcagg agactttttc tttgctgttg cagccttctg cggacgatat
     2221 ttcagggcag agtgtgcggc tgccaggcgt gcaatcggca cacggaatgg cgagcaagag
     2281 acatagttgt tgccgatgat atgacaccat tcaatggagc tagggtcacc accatgttca
     2341 ccgcagatac ccacttcaag atttggtcgg gttttgcgac cttcagcgat tgcccactcc
     2401 atcagtttac cgacgcctgt gcggtcgagc gtctggaatg gattcttctc aaggattccc
     2461 tgctcctgat acgtgatcag gaagttgcgc tcggcatcat cgcgggaata accgtaggtc
     2521 atttgcgtca ggtcgttggt gccaaaggag aagaattcag cccgctgagc cacttcttca
     2581 gcggtgacag cggcgcgcgg gatttcgatc attgtgccaa acttatattc aaatttgacc
     2641 tttttttcct gcatgacagt tccagcgatg cgttcgagac gaggctgaat ccattcgagt
     2701 tccttcacgg ttccggtcag agggatcatc acttccggtt tgactacgat gccgcgtttt
     2761 gtacaatctg cagcagcctc aaagatggcg cggacttgca tctccacgat ctctggcatg
     2821 gcaatgctca gtctgacgcc gcgcaagccc atcatcgggt ttgattcgtg caatcctttg
     2881 atcgcagata acagatcctc tttttccttt agcccatccg attcattttt cacgcgcaga
     2941 gtaatgactt cttcaagaag tttttcttcg tctggcatga attcatgcaa aggcgggtca
     3001 atcaggcgga tgattaccgg gtagccgtcc atcgcttcaa agagcccgtc gaagtccttc
     3061 cgctggaagg gcagtaattc gctaagggcg acggttcttc cttcactgct tcccgataag
     3121 atcatacgct ggacaatggg caaacgctca ggctcaaaga acatgtgctc ggtgcggcaa
     3181 agaccgattc ccactgcgcc ataagagcgg gcgcgctgcg cgtcctttgg atagtccgca
     3241 ttcgcccaca cttgaagacc gcgcgtcgga taacctttgg cggatgcggc acgaatatct
     3301 ttgcgtgcgc aaatatcatc tgcccatttg agcaaagtca acagatcagt ctgttcatca
     3361 agagaaggag ctgatgtcgg aatttttccg agaaacacct ttccggttgt gccatccaca
     3421 gaaatccatt caccctcttt gaccacagca tcaccgaccg tcatttgccg tctttccagg
     3481 tcgattttga tggccgacgc gccgaccaca cacggaatac caaactggcg agccacaacc
     3541 gctgcgtgag acgtagcccc accttcgctg gtgagcacac ccttcgaggc gatcatgcca
     3601 tgcacgtcat caggcttcgt gaatggacgc accatgagcg tatcctgctt ttcttccttt
     3661 gccattcttt cggcagtatc agcatcgaaa tagacttgac ctacggcggc gccgggagag
     3721 gcgttgacgc cttttgcaag gaacgtgcct gtcttttctg cctcattcat ggcggtttca
     3781 tcgaattgag ggtgaagcag ggaatcgaca ttatctggcg tgacgcgcag aacggcctgc
     3841 tccttcgtga tcaggccctc gtttgccata tcgaccgcaa ccttcaccgc ggacttggcg
     3901 gtgcgcttgc cgcttcgggt ttggagcatc cagagcttgc cgcgttcaat cgtgaactca
     3961 acatcctgca ggtctttgta atgcttttcg agtcggcttg aaatggtcat gaactgctta
     4021 tatgcagcag gcattttctc tttt
//
LOCUS       DASTJP010000839         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_81677, whole genome shotgun sequence.
ACCESSION   DASTJP010000839 DASTJP010000000
VERSION     DASTJP010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81677"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFQ23_24430"
     CDS             complement(<1..249)
                     /locus_tag="VFQ23_24430"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9909818.1"
                     /translation="MPIKYSCFISYKHDSVNIARDFQDALENEISLLLNLPVYRDEER
                     LHAGDFFNSALAQALCQSACMVMIYIPRYFDKASTYCAR"
     gene            complement(401..3013)
                     /locus_tag="VFQ23_24435"
     CDS             complement(401..3013)
                     /locus_tag="VFQ23_24435"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9909819.1"
                     /translation="MPLASLLDFWKYDADTAPNLVAWRTLPPRPAQTAPFPDDLPAPI
                     KQTLIASGIHSLYSHQLEAWTHTRAGENIILSTSTASGKTLAYNLPVFASLLQNSNAR
                     ALYLFPTKALTQDQLSNLQAIQTNLAPSIYDGDTPQSQRSAIRKNARIVLSNPDMLHT
                     GILPHHTNWLEFFTNLRFVVIDETHTYRGVFGSHVANVIRRLKRVANFYGANPQFILA
                     SATIGNPKELAEKLIEEPVQLIDNDGSARGPRHFIIYNPPIIDESLGLRKSSLLESVR
                     LAQDLLRNDIQSVVFARSRRSVEIVLRYLQESPSPPTPLPSGEGRRGEGEIRGYRSGY
                     LPHQRREIEKGLRDGTVKTVVATNALELGIDIGGLGAAILVGYPGTVASARQQAGRAG
                     RGLESAAAVMVASANPIDQFLAHHPEYFFERSPEQALVNPDHLLILLEHLRCAMFELP
                     FQKGEGFGAVSPQMIEDYLNFLVENKEAHLSKEKYYWMADQYPAANISLRSASPQSVV
                     LQTTIDDRPLTIGTVDGESATWMTHPGAIYLHEAQSYFVKELNLEEHIARLHPIESDY
                     YTEPLQQTSIEVLSVASESPSPTRAERTLSGGEVEDEAERESGGEGENRATKSWGELQ
                     VTTQVTGFRKRRWYTHDNLGEEPLDMPPSDLQTTGYWLSLSEETVNRLREMGAWTNDP
                     NNYGPEWTRIRDRVRARDKYTCQVCGTPETTRQHDVHHKVPFRAFTSLAEANRLDNLT
                     TLCPSCHHKAEQNVRMRSGLAGLAYVLGNLAPLFLMCDSGDLGSHIEPVENKTFGQPT
                     VVLYDSIPAGIGFSQKLFEMHDELIARALELVSECACADGCPSCVGPGGENGYGGKQE
                     ALAILRELIRDTGG"
     gene            complement(3022..>3601)
                     /locus_tag="VFQ23_24440"
     CDS             complement(3022..>3601)
                     /locus_tag="VFQ23_24440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HET9909820.1"
                     /translation="RRNDLDPSEIAASFVILIIAVGLGSLLYLGSRSGDALGNALARL
                     ARLVNRVARPFIHRDYLREERAHEFAHEMAADLKSLPERPRSLIKPLLLAFAGKTLMV
                     CILAVSFLSFNVPVSIETIIGGFAISYLFLIVSPTPSGIGIVEGIMPLALNSLNVAWS
                     QAVVITLAYRAVTFWFPLGVGAWAFRHLHMQE"
BASE COUNT          826 a          876 c         1017 g          882 t
ORIGIN      
        1 acgagcgcaa taagtgctgg ctttatcaaa gtatcttgga atatatatca ttaccatgca
       61 cgcgctctga cagagagcct gtgcaagggc agagttgaaa aagtcccctg catgtaaacg
      121 ttcttcatcc ctgtaaacag gcaaatttaa tagcagagaa atttcgtttt ctaaagcgtc
      181 ttgaaaatct ctggctatgt tcacgctgtc gtgtttgtaa cttatgaagc aagagtactt
      241 tatcggcata ttgcttttcc tgttacttat ggctttattt taacaccaac tatcctcttg
      301 ttccgcttta gcgtaaatta ctatattaag aataatatca accgagatcc ttcatgatta
      361 ttggtggttt cgatacgttc ctcgctgtcg ctcggaacac tcaaccacct gtatctctga
      421 tcaactctct caagatcgca agcgcttctt gtttccctcc atatccgttc tcgccgccgg
      481 ggcccacgca tgagggacaa ccatccgcgc aggcgcattc actcacgagt tcaagtgcgc
      541 gtgcaatcag ctcatcgtgc atctcaaata gcttctggct gaaaccaatc cctgcaggaa
      601 ttgaatcata tagaaccaca gttggctgac cgaaggtctt gttttcgacg ggttcgatgt
      661 gtgagcccag gtcacctgaa tcgcacatca ggaataatgg cgcaaggttc ccgagcacat
      721 aggcgagccc ggccagcccg cttcgcatgc ggacgttttg ttccgctttg tgatggcagg
      781 aggggcagag cgttgtgagg ttatccagtc gatttgcctc tgccaatgaa gtgaaggcgc
      841 gaaaaggaac cttgtgatgc acatcatgtt gtcgggttgt ctcaggtgtg ccacagacct
      901 ggcatgtgta tttgtctcgg gctcggacgc ggtcacgaat gcgagtccat tcgggtccgt
      961 agttgtttgg gtcgttggtc cacgctccca tttcgcgtaa acggtttaca gtctcttcgg
     1021 agagggaaag ccagtagcct gttgtctgca gatcgctggg aggcatgtcc agtggttctt
     1081 cgccgagatt gtcatgcgtg taccagcggc gcttgcgaaa gccagtgacc tgggtagtga
     1141 cctggagttc gccccaggat ttggttgctc tgttttcgcc ctcacccccg gactctctct
     1201 ccgcttcgtc ttccacttcg cctccgctca gtgtccgctc cgcgcgagta ggagaagggg
     1261 actcgctcgc gacagataac acttcgatgc tggtctgttg aaggggctcg gtgtagtagt
     1321 cgctttcgat gggatgcagg cgggcgatgt gttcttcgag attgagttct tttacaaaat
     1381 aggattgtgc ttcatgcaaa tagatcgcac cggggtgtgt catccatgtg gcgctttcgc
     1441 cgtcgaccgt gccgatggtc aatggtctat cgtcgattgt tgtttgaagc acaacacttt
     1501 gcggagaggc cgaccgcaac gaaatattcg cagcgggata ttgatctgcc atccaatagt
     1561 atttttcttt cgagaggtgc gcctctttgt tttcaaccag gaagttcagg taatcttcaa
     1621 tcatttgcgg gcttaccgca ccaaaacctt ctcctttttg gaatggcaat tcaaacatcg
     1681 cacaacgcaa gtgctccagc agaatcaaca aatgatcggg attgaccaac gcctgctcag
     1741 gtgaacgctc gaagaaatat tcggggtgat gtgcgaggaa ctggtcaatg ggattcgcag
     1801 acgccaccat cactgctgcc gcggattcga gtccgcgccc tgctcgaccg gcttgctgcc
     1861 gcgccgaagc gacagtgcct gggtagccga cgaggatcgc cgcgcccagc ccgccgatgt
     1921 cgattccgag ttctagggcg tttgtggcga cgacggtttt gaccgtgccg tcgcggagtc
     1981 ctttttcgat ttcgcgacgt tggtgaggga ggtaacctga gcggtagccg cgaatttctc
     2041 cctcacccct tcgcccctct cccgatggga gaggggtagg gggtgaggga gattcctgca
     2101 aatatctcaa aacaatttca acacttcttc tggagcgagc aaagaccaca gactggatat
     2161 cgtttcgcag caagtcttga gccaggcgga cactttcgag gagagacgat tttctcaagc
     2221 caagtgattc atcgatgatc ggcgggttgt agatgatgaa gtgacgtggt ccgcgggcgg
     2281 agccatcgtt gtcgatcagt tgaacgggct cttcgatgag tttttctgca agctcttttg
     2341 ggttgccaat cgtggcagac gccagaatga attgtggatt tgcaccgtaa aaattcgcaa
     2401 cgcgtttgag tcggcgtatc acgtttgcga catgtgaacc aaaaacaccc cggtaggtat
     2461 gggtttcgtc gatgacaaca aaacgcaaat ttgtgaagaa ctccagccag ttggtgtgat
     2521 gcggaagaat gcccgtgtga agcatatctg ggttgcttaa tacaatgcgc gcgtttttac
     2581 gaatagcgga acgttgtgat tgtggcgtat caccatcgta gattgagggc gccaggtttg
     2641 tttgtatcgc ctggagattg gagagttgat cctgggttaa ggctttcgtg gggaagaggt
     2701 aaagcgctct cgcattgctg ttttgaagca gggatgcaaa gacgggcagg ttataagcga
     2761 gggtcttccc gctggctgtg ctggtggaaa gaataatatt ttcacctgcg cgggtgtgag
     2821 tccaggcttc gagttggtgt gagtagagag agtggattcc agatgcgatc agagtctgtt
     2881 taatgggagc aggcagatca tccgggaagg gagctgtttg tgcgggacgc ggaggcagcg
     2941 tccgccaggc cacgagattg ggtgcggtat ccgcgtcata tttccagaaa tccaaaagag
     3001 aagcgagggg catgctgtat cttattcctg catatgcaag tgacggaatg cccaggcgcc
     3061 cactcccagc ggaaaccaga atgtgactgc gcgatacgca agcgtaatga cgactgcctg
     3121 gctccaggcg acgttgaggg aatttaacgc cagcggcatg atgccttcga cgatgccaat
     3181 gccagaaggg gttggcgaga cgatgaggaa gagatacgaa atggcgaacc caccaatgat
     3241 cgtctcaatt gaaaccggga cattgaacga caggaaggat actgccagaa tacagaccat
     3301 cagcgtcttg cctgcaaacg caaggagcag cggcttaatc aagctgcgcg gccgctcggg
     3361 aagagacttt aagtctgcgg ccatttcatg ggcgaattcg tgtgcccgtt cttcacggag
     3421 ataatcacga tggataaagg ggcgtgccac gcgattaacg agtcgcgcca aacgggccag
     3481 cgcgttgccc agggcatcac cggaccgtga accaagataa agaagcgacc caagccccac
     3541 agcgataata aggataacaa acgaagctgc aatctcactg gggtcaaggt cgttgcggcg
     3601 g
//
LOCUS       DASTJP010000840         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_713978, whole genome shotgun sequence.
ACCESSION   DASTJP010000840 DASTJP010000000
VERSION     DASTJP010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713978"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFQ23_24445"
     CDS             <1..687
                     /locus_tag="VFQ23_24445"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET9909821.1"
                     /translation="LTEQVAPADLIYESNEDETDRFLIKKEISFGGISRIRRTGDSWG
                     RGEPDRAAKKLLGALLADGEVIEVQVEGWKTIHHALASDTKVLRDVGAGRIPKAWTPL
                     ETTTMEEVVFLAPLDPVSARGRAKALFDFDYVWEVYKPEHQRKFGYYTLPILWGDKLV
                     ARFDSKLNRSTNTFVILGLWLENKALGKDEAFAEALARGFARFVKFLGANKMDAKAIR
                     EPLLRKRVSA"
     gene            complement(684..1139)
                     /locus_tag="VFQ23_24450"
     CDS             complement(684..1139)
                     /locus_tag="VFQ23_24450"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9909822.1"
                     /translation="MKAIILETRDWVNIVAVTPEKKIVTVKQFRFGVAKTTVEVPAGI
                     IEKGETPEQAAIRELKEETGYTTNHWQYLGWFEANPAFLNNVCHVWLALDVVKTQSTQ
                     LDDGEEISISELSMEEVRREIEQGRMRNSLTLLALSRVFDLRDPEQKSG"
     gene            complement(1293..2105)
                     /locus_tag="VFQ23_24455"
     CDS             complement(1293..2105)
                     /locus_tag="VFQ23_24455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9909823.1"
                     /translation="MKVQNKVIVVTGGGNGIGRELVLNLLSKGARVAAVDVNESALQE
                     TVALAGNHKDKLSTHVVDITNKDAVEALVEKVISAHGTVDGVINNAGIIQPFVRVNEL
                     SYADIDRVMNVNFYGTLYMTKALLPHLLKRPEAHIANVSSMGGFLPVPGQSLYGASKA
                     AVKLFTEGLHSELLTTNVGVTVIFPGAIGTNIAANSGVANTLRVENVDQAEMARRTLA
                     PSKAAEIIVNAIEKNLFRVTVGSDATFLDRFYRLSPERAANFIFKQMQSLLG"
     gene            complement(2418..2873)
                     /locus_tag="VFQ23_24460"
     CDS             complement(2418..2873)
                     /locus_tag="VFQ23_24460"
                     /inference="COORDINATES: protein motif:HMM:NF038048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="HET9909824.1"
                     /translation="MKLAEALVLRADKKKRIEQLKQRILRNAKVQESQKPAEDPESLI
                     KEMEKISKELVELIQRINKTNSSTELEKGVSLADAIASRDILVLKNSIYRDLAQAATI
                     TQDIRTKSEVKFKGTVKVAQIQERADELAKAHRELDTKIQEMNWKTELQ"
BASE COUNT          708 a          761 c          693 g          861 t
ORIGIN      
        1 ctcacagaac aggtcgcgcc cgcggacttg atttacgaga gcaatgagga tgagaccgat
       61 cgtttcctga tcaaaaagga aatcagcttt ggtggcatat cgcggataag acgcacaggt
      121 gattcgtggg ggcgcggcga accggaccgt gccgcgaaga agttgctagg cgcgctgtta
      181 gcggacggcg aggtcatcga ggtgcaggtc gagggctgga aaacgatcca tcacgcactc
      241 gccagcgaca ccaaagtact gcgcgatgtg ggcgccggtc gcattccgaa ggcatggacg
      301 ccgttggaga caaccacgat ggaagaggta gtttttctcg cgccgcttga ccccgtcagt
      361 gcacgcgggc gagcaaaagc tttattcgac tttgattatg tttgggaggt atacaaaccc
      421 gagcaccagc gcaagtttgg ttattacaca ttaccgatcc tatggggcga caagcttgtc
      481 gctcgcttcg acagcaaact taaccggtcg acgaacacct ttgtcatact gggcctatgg
      541 ctggagaata aggctctcgg aaaagacgag gcgtttgcag aggcattggc gcgtggtttc
      601 gctcgcttcg ttaaatttct cggcgccaac aaaatggatg cgaaagcgat tcgcgaacca
      661 ttgctgcgta agcgagtaag tgcctaacct gatttctgtt ctgggtcccg cagatcaaac
      721 actcgcgaga gggctaatag ggtaagggaa ttgcgcattc tgccctgctc aatttccctg
      781 cggacttctt ccatcgatag ctcactgatt gaaatctcct ccccgtcatc caattgagtg
      841 gactgtgttt tgactacatc cagagctaac cagacatgac atacgttatt taaaaatgcc
      901 gggtttgcct caaaccagcc aagatattgc cagtgatttg tggtatatcc ggtttcttcc
      961 tttaactcac ggattgcggc ttgctctggc gtttctcctt tttcaatgat acctgctggt
     1021 acttcgaccg ttgttttcgc tacgccaaac cggaattgtt tcaccgtgac aattttcttt
     1081 tccggggtca ctgccacgat attgacccag tctcgcgttt cgagaattat agctttcatg
     1141 agttttgcat tacgagggtt tcgaacccag tcgtaccttg tctggaacaa aaccagatcc
     1201 ggtccctgtt ctgattgaat cacttcccaa gactgtggcg tatctgcatc catatttgaa
     1261 agtatcctat tgatcatcac cgtttgtatt tatcatccca acagcgattg catctgcttg
     1321 aaaataaaat ttgccgcgcg ttcggggctg agtctgtaga aacgatcgag gaaggtagca
     1381 tcggagccaa ccgtaacgcg gaagaggttt ttctcaatag cattcacaat gatttctgct
     1441 gccttgctgg gtgctaatgt ccttcttgcc atctctgcct gatccacatt ttccactctc
     1501 aatgtgtttg ccactccgga attggcagca atatttgtgc caatcgcgcc gggaaatatg
     1561 acggttaccc ccacattcgt agtcaacagt tccgagtgta atccttccgt aaataacttc
     1621 acggccgcct ttgatgcccc ataaaggctt tgcccgggga ctggcaggaa accacccatg
     1681 ctggacacat tggcaatatg ggcttcaggc cgctttagca ggtgcggcaa gagtgccttt
     1741 gtcatgtaga gggttccata aaaattgaca ttcattactc ggtcgatgtc ggcataactc
     1801 agttcgttga cccgaacgaa aggttgaatg ataccggcgt tgttgatcac gccatccacg
     1861 gttccatgtg cggagatcac cttttcaacg agggcttcca ctgcgtcttt gttcgtaata
     1921 tccacgacat gtgtggataa tttgtctttg tggtttcccg caagcgcaac agtctcttgt
     1981 aaagcagatt cgtttacgtc aacggcagcg actcttgcac ctttggacaa tagatttagt
     2041 accaactctc tacctatgcc gtttccgcca cctgtgacca cgatgacttt attctgaact
     2101 ttcatttcta ttttctcctt agtttccgta tgtgattgtt tgatatggtg cacgccgccg
     2161 aggatcacat ccatgctgaa taaggcacag tcatgcaact gcacctaaaa gccccggacc
     2221 cgccctcacc attctggcac gttagcacga ggtagttact tttacaatac cctgtacacc
     2281 tgtaacgtta ctgggcatac cctgtataag gtgggattcg aacccactag gcaccgcccc
     2341 aagcacctac cggcgccaac tttgccccag tcggcagggg ttgtgctcac ccatgcagaa
     2401 tggctaccaa ctaattttta ttgcaactca gttttccagt tcatttcctg tattttggtg
     2461 tcgagttcgc gatgagcctt tgcaagctcg tctgctcgct cctgaatctg tgcaaccttt
     2521 actgtgcctt taaatttcac ttcagatttt gttcgaatat cctgtgtaat agttgcagcc
     2581 tgagccaagt cacgataaat agaattcttc aagacgagga tatcacgact agcaatagca
     2641 tctgcaagag aaacgccttt ttctaattcg gtgctcgaat tcgtcttgtt gatacgttga
     2701 attaactcaa ccagttcctt tgatattttt tccatctcct taatcaagga ttctggatct
     2761 tcggctggct tttgactttc ctgaactttt gcgttgcgaa ggatacgctg tttgagctgc
     2821 tcaattcttt tcttcttatc agctctcaaa accaatgctt ctgctaattt cataatattt
     2881 tcccttcgat tttggtgatc agttatgctt catacgaagt gctgagaatg agcttgccat
     2941 cgagaataag attgttagga aacgtatttg attcgctcaa ttattttcac tgaacttcct
     3001 tcaccaaaga tacaccctct gaa
//
LOCUS       DASTJP010000841         3936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_876226, whole genome shotgun sequence.
ACCESSION   DASTJP010000841 DASTJP010000000
VERSION     DASTJP010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876226"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFQ23_24465"
     CDS             <1..741
                     /locus_tag="VFQ23_24465"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9909825.1"
                     /translation="FINQFTSAGYQVIRLDYLGTGLSDWISNWNKNNAYSLFDMAKDV
                     ISVIDTLHISRVHLFGFSLGGMIAQEIAINNPERISSLTLMMTTGDATDSKMKTMSMT
                     YVFSSMIRSVPIIKYRILGGERNLIIERILKMQAAQMNFDVKETAQQVLYDLRNRKGI
                     NFKAVFQHFKAVEISGSRYEKLKDIKTPTLIIHGTKDNIFPIEHGRKLQELIPNTKSI
                     WLDGVGHMLSVPNMDSLVYKVIEHMSAL"
     gene            915..1280
                     /locus_tag="VFQ23_24470"
     CDS             915..1280
                     /locus_tag="VFQ23_24470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909826.1"
                     /translation="MKFAKIGAILYIIWGLLHIKAAFDEFALGASVDPGLIRGKLHQG
                     AWDLLFFALASIAIAIIFNWKNDVRGYWINLFLVSIADLGFIIFVLIPGYVAFFPGIL
                     GPVFWVSAAIFSSIGNRSR"
     gene            1563..2987
                     /locus_tag="VFQ23_24475"
     CDS             1563..2987
                     /locus_tag="VFQ23_24475"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HET9909827.1"
                     /translation="MENLKDETVTKDAIFQDRTRLVQADYLVVGAGAAGMAFVDSLIH
                     NANVHVALLDRRHSAGGHWLDAYPFVRLHQASEFYGVVSTPLGKGERQTDGPEAGLYQ
                     RAAAPEIVAYYARVVKNMVTSGKVSFHPNSEYLGGRRWISRVSGQNFAVPDRCRVVNA
                     HYQAPDIPSQSPAPFGVADGVHVIPVNDLVRLPEIPGQFVIVGSGKTATDAIVWLLDN
                     GVDPGTVCWLRPRDPWMFNRAVVQPDPAIYLGMVADILEAAGDATSPDDLFLRMEAAG
                     VMLRIDRRVTPTMAKTPTLAQWELDRLRAIENVVRLGHVRYVEPGRVMCAGGEAAIDR
                     DAVVVHCAASGLRYEPLIPVWGSEAITLQSVAVGPIFGAAIAGYVEATREDDAEKNRL
                     CPPHPFSNTPADWPQLIVRGAQGSQALATESDVKRWSHATSLYRSRIPAERANDPDVA
                     AAAARVEAVAGIGMARLAELGRMQ"
     gene            3309..3755
                     /locus_tag="VFQ23_24480"
     CDS             3309..3755
                     /locus_tag="VFQ23_24480"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909828.1"
                     /translation="MMTTKTILTRVATSDDVQDLARLNAKFNGSSDPPEYLAMRLADR
                     FCVEQPLLAEVDGHVVGFAALRIVPCVFYADPHGELTELFVEEPYRRQGVGRELLRLA
                     EKIAKEKGVKELLILTDASNQAAQTFYNSMDYEDGELAFKKELAKS"
BASE COUNT          932 a          988 c         1060 g          956 t
ORIGIN      
        1 tttattaatc agtttacatc tgcaggatat caagtgatcc gattagacta tctaggcact
       61 ggattatcag attggataag taactggaat aaaaataatg catactcgct ttttgacatg
      121 gcaaaggatg taatatccgt aattgataca ttgcatatta gccgtgttca cctttttgga
      181 ttttctcttg gaggtatgat tgctcaagaa atagcaatta acaatccgga aagaatttct
      241 tctcttactt taatgatgac aacaggcgat gccacagatt ccaaaatgaa aacaatgtcc
      301 atgacctacg tttttagttc aatgataaga tctgttccaa taataaaata tcgtattctt
      361 ggaggtgaaa ggaatttaat aattgaacga atactaaaaa tgcaagccgc acaaatgaat
      421 tttgatgtca aagagacagc acaacaggta ttatatgatt tgagaaacag gaaagggatt
      481 aattttaagg cagtctttca gcattttaaa gctgttgaaa tatctggatc gagatatgaa
      541 aaattgaaag acataaaaac accaacactt atcatacatg ggacgaaaga taatatattc
      601 cccatagaac atggaagaaa attacaagaa ctaattccaa acacaaaatc aatctggtta
      661 gatggtgtag gtcatatgct ctcagtgccc aatatggatt ctttagttta taaggttatc
      721 gaacacatga gtgcgctcta acacagcatc cagcggacgg ctgagagtct gcgcgttttc
      781 aggcattttc tggcttcggg cttttctccg tttcggcggc gagtccacgc tcccacccca
      841 agcggctaac gccagccgtt gggcgcttgg agattggcag ctttagaagc cgaaaatata
      901 aaaaggagca gcttgtgaaa ttcgctaaaa taggcgcgat cctgtatatc atttggggtt
      961 tacttcatat taaagctgcc ttcgatgaat ttgctcttgg cgcaagcgtc gatccaggat
     1021 tgattcgcgg aaaactacat caaggcgcat gggacctttt gtttttcgcc ttggccagta
     1081 ttgcgattgc catcattttc aattggaaaa atgatgtgag gggatattgg attaacctgt
     1141 ttctagtaag tatcgctgat cttggtttca ttatctttgt tctcatcccg ggttatgtgg
     1201 ctttctttcc tggtattttg ggacccgtat tttgggtatc cgcagccata ttctcaagca
     1261 ttggcaatcg atcgcgttga catttgggtc atcattcatg tcgagaaatc agtgcctggt
     1321 tgtcaaactg tcggaagcga ggcgcccaat aacggctgca gggggacttg gtggcacgcg
     1381 ccacgctaga accgccgttc ggcggcaatt gacattctct gcagcttttc gattgctccg
     1441 gaggtaccac ttattttcaa gcaacacaat cccaatctat gactcgcccc tcgcgtcaga
     1501 aaagagtgca cggattttat ggatcaacgc cgatattgac gcggcatgag gagaatcccg
     1561 aaatggaaaa tcttaaggat gaaaccgtaa ctaaggacgc catctttcag gatcggacta
     1621 gactggtgca ggccgactac ctggtcgtcg gcgctggtgc cgcaggcatg gcgttcgtgg
     1681 attccctcat ccacaacgcc aacgtccacg tggccctact cgaccgccgc cacagtgcgg
     1741 gcggtcactg gctcgatgcc tatccgttcg ttaggctgca tcaggcatcc gagttctatg
     1801 gcgtggtctc gaccccgctg ggcaaaggcg agcggcagac tgacggaccg gaggcgggct
     1861 tgtaccagcg ggctgccgcg ccagaaatcg tggcctacta cgcacgtgtg gtcaagaaca
     1921 tggtcaccag cggcaaggtc tccttccatc ccaacagcga atacctcggc gggcgacgtt
     1981 ggatttctcg ggtgtcggga caaaatttcg ctgtgccgga tcgttgccgc gttgtcaacg
     2041 cacactatca ggcaccggac attccgtccc aaagcccagc acccttcggc gttgcagatg
     2101 gcgtacacgt aatcccggtc aacgatctgg tcaggctgcc tgagataccg ggtcagttcg
     2161 tgatcgtggg ttctggcaag acagcgaccg acgccatcgt ctggctgctt gacaatggtg
     2221 tcgacccagg cacagtctgc tggttacgcc cgcgagaccc ctggatgttc aaccgcgcgg
     2281 ttgtgcagcc tgatccggcc atctatttgg gcatggttgc cgacatcctg gaggcggcgg
     2341 gcgatgcaac ttcaccggac gatctcttcc tgcgcatgga ggcggcagga gtgatgctgc
     2401 gtatcgatcg gcgcgtcact cccaccatgg cgaagacccc gaccttggca caatgggagc
     2461 tggaccgcct gagggctatt gagaatgtgg tgcggttggg tcacgtccgc tacgtggagc
     2521 cgggtcgagt tatgtgcgcg ggcggcgagg ccgcaatcga ccgagatgcg gtggtagtgc
     2581 attgcgctgc ttcgggcctg cggtacgaac cgttgatacc ggtctgggga tccgaggcga
     2641 tcacgcttca gtccgttgca gtcggaccga tctttggtgc cgcgatagca ggctacgtgg
     2701 aagccacccg agaagacgac gccgagaaaa accgactctg cccaccacac cccttctcga
     2761 acacgccggc tgactggccc cagctgatcg tccgaggtgc tcaaggatca caagcgttgg
     2821 ccacagaatc ggatgtcaag cgttggtcgc atgcgaccag cctgtaccgg tctcgcatcc
     2881 cggccgagcg agcgaacgac cccgatgttg ccgccgccgc tgcacgggtg gaggctgtag
     2941 cgggaatcgg catggcaagg ctggccgaac tgggcaggat gcagtagccc gttaggcgcg
     3001 gcctgtttgt gtctaccgtc ggtctggcct gtagtagcgc gaatggcgac gacaggattt
     3061 agactagaaa atgatcattg ccactttctg cacctccctg tgtccgtcag cgagggtgcg
     3121 cgcctggtct catccacgca gtctaatgct tgcaagatgg cggcgggtgc gcaacgctcg
     3181 cttgacaggg tgggttctca acccgtccac cagtactcct gaaagggcat accctttaca
     3241 ggatgctacg caaacgccaa ccgttaagcg ctcagcgaaa atcatgtgat gcttcctaag
     3301 aggttattat gatgaccacc aaaacgattc tcaccagagt cgcgacgtcc gatgatgtgc
     3361 aagatttggc acgtttgaat gctaagttta atggcagctc tgatcccccg gaatacttgg
     3421 caatgcggct tgccgatcgt ttttgtgtgg agcaacctct gctcgctgaa gttgatggtc
     3481 atgtggttgg gtttgccgcc ctgcggatcg tcccttgtgt cttttatgcc gatccccatg
     3541 gcgagttgac tgagttgttt gtcgaagaac cgtatcggcg gcagggtgtc ggtagggaat
     3601 tgctccgctt ggcagagaag attgccaagg aaaaaggcgt gaaggagtta ttgattctca
     3661 ctgatgcctc gaatcaggcc gcgcaaacat tttacaattc catggactat gaagacggcg
     3721 agctagcctt taagaaagaa ctagccaaat catgagaagc cttccagacg tgcgccaaac
     3781 acagccatca tcgctccgca cgtcgctatt atgacagcct tcagcgtgca ctggacgctg
     3841 ggggtgccaa gcgccaatgt gcttttgctg ggcaatccca tccgctacgc tcctggaaca
     3901 ggtgtgctcg cttgaagttc gcttgctcaa tctgcg
//
LOCUS       DASTJP010000842         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_186706, whole genome shotgun sequence.
ACCESSION   DASTJP010000842 DASTJP010000000
VERSION     DASTJP010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186706"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VFQ23_24485"
     CDS             <1..1532
                     /locus_tag="VFQ23_24485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9909829.1"
                     /translation="TGNLGAVFTQIYRYKRVSNSVEKEQIKWVVYGLSLCVIGYLISG
                     SVILNLGDPNGSDLSIKLLFVVVSTLSVLLIPISIAVAILRYRLWDINPLINRTVVYA
                     SLTALVVGIYVLIVSYLSFLFRTQSNFFISLIATGMVAVLFQPLREWLQQGVNHLMYG
                     ERDEPEVVLSRLGQRLEASLEPDSVLPSVVETVGQALKLPYVAIALKDGTDFALAAEY
                     GRFVTSDESKADLTKMPLTYQSETVGELIVAPRASNERFSPADKKLLSILSQQAGAAA
                     HTIRLTHELRNLNLDLQKSREELVTAREEERRRIRRDLHDGVGPILASLLQRLDAVRL
                     LIPRDPEKAASIAEALKGQVKSTIADIRRLVYALRPPILDELGLVSAIREYTADYQGA
                     GSLQVTLNAPETLPRLPAAVEVAAYRIVLEAFANVVHHSQATKCVITIRLEAQQTASL
                     FCVDIQDNGQGLPEKHHAGVGMVSMRERTSELGGSIRIEASRSTGTRILACLPFSEEE
                     K"
     gene            1529..2191
                     /locus_tag="VFQ23_24490"
     CDS             1529..2191
                     /locus_tag="VFQ23_24490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9909830.1"
                     /translation="MTIRVLIADDHLFYREGVRTLLNTAEGIEIVGEASNGHEVVARA
                     AELQPHIILMDLKMPGPNGIDATRQIIQKQPNVGILVVTMFDDDESVFAVMRAGARGY
                     ILKDADQEELVRAVTAVFRGEAIFSPTIAQRMMNYFSNVSREGRSKADTAFPELTERE
                     LDILELIAQGHGNPAIANKLSLSVKTVQNYVSNILNKLQVVDRSEAIVRAREKGLGVD
                     KK"
     gene            2733..>3521
                     /locus_tag="VFQ23_24495"
     CDS             2733..>3521
                     /locus_tag="VFQ23_24495"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HET9909831.1"
                     /translation="MHFYAVSIGSTVPQNNFTAAIQSVFESSLNLRLGHQDRLITILV
                     SDHYDLPQGIRLIARNVPFQSLVVGLRAASTEKILRFESSPLLISLREASIWEAQIPY
                     IDVTLASTRQAWARLWHSLNLQQRLKGTELIGEDLFQTEQGSHLTRKLSQPVLGLIAG
                     TKDYDVETAVDAAQKMIGLGPGVTPTGDDILIGYLAGLWSATGDDNQQIKFVEAFGNA
                     LLNLIRQTNEISRTYLYHAIHKQFSSSLIKLLNAIREGEPDQIQT"
BASE COUNT          869 a          831 c          915 g          906 t
ORIGIN      
        1 tcactggcaa ccttggcgcg gtcttcacac agatctatcg atacaagcgc gtatcgaact
       61 ctgtggagaa agaacagatt aaatgggttg tctatggctt gagtttgtgt gtgattggat
      121 atctgatcag cgggtcggtt atccttaatt tgggcgatcc caacgggagc gacctgtcga
      181 tcaagcttct ttttgtcgtg gtttcaacgc tgtctgtttt gctcatcccc atttccatcg
      241 ccgtggcaat tcttcgttac cgtttgtggg atatcaatcc tctgatcaat cgtactgtgg
      301 tctatgcatc gcttaccgcg ctggtggtag gcatctacgt tttgatcgtt agttacctat
      361 cattcctgtt tcgaacgcaa tcgaatttct ttatctcgct aattgcgact ggaatggtgg
      421 ctgtgctatt tcagcctttg cgagaatggt tgcagcaggg tgtaaaccat ttgatgtatg
      481 gggaacgtga tgaaccggag gttgtcctct cccggctcgg acagcgcctc gaagccagcc
      541 tggaacccga ttccgtattg ccgtccgtgg tggagacggt ggggcaggca ctgaaacttc
      601 cttatgtcgc gatcgcgttg aaagatggga cggacttcgc tctcgctgca gaatacggtc
      661 gtttcgtgac atctgatgag tcgaaggcgg atcttactaa aatgccgctc acgtaccaat
      721 cggaaaccgt tggggaattg atcgtggcac ctcgtgcttc caatgaaagg ttttctcccg
      781 cagataaaaa gttgctatcc atcttgtcgc agcaagcggg tgccgctgcc cataccatcc
      841 gccttactca tgaattacgc aacctcaacc tggatctgca gaaatctcgt gaggagctgg
      901 tgactgcacg cgaagaggaa cggcgccgga tccggcggga tctgcatgat ggcgtggggc
      961 cgatcctggc aagcctgttg caacgcctgg atgcggtccg tctgttgatc ccgcgtgacc
     1021 cggaaaaggc tgccagtatc gcggaagcac tgaaaggaca ggtcaagtcc acgatcgcgg
     1081 acattcgccg tctagtgtat gccctccgtc cgcccatatt ggatgagctg ggcttggttt
     1141 cagccattcg cgaatataca gcagattatc aaggtgcagg cagcttgcaa gtcaccctga
     1201 atgctcccga aacattacca cgtttgcctg ctgcggtcga agtggctgcc taccggattg
     1261 ttctggaagc gttcgcgaat gtggttcatc attcgcaagc aacaaaatgt gttattacca
     1321 tccggctcga agcacagcaa acggcctccc tgttttgcgt tgatatccag gacaatggcc
     1381 agggcttgcc tgagaaacat catgcaggtg tggggatggt ctccatgcgc gagcggacat
     1441 cggaactggg aggcagcata cgcatcgaag cgagcagaag cacagggaca aggatcctgg
     1501 cttgcctacc atttagtgag gaagaaaagt gacgataaga gttttgatcg cagatgacca
     1561 tcttttttac cgcgagggag tgcgcactct gttgaatacg gcagagggta ttgagatcgt
     1621 cggagaagcc agtaatggac acgaggttgt ggcacgcgca gcagaacttc aacctcatat
     1681 catactgatg gatttgaaga tgcccggtcc aaatggtatc gacgcgacgc ggcaaattat
     1741 tcagaaacaa ccaaacgtgg gcatactggt tgtgactatg tttgatgacg atgaatccgt
     1801 ctttgcagtg atgcgcgcgg gcgcacgtgg ttatattctc aaggacgcgg accaggaaga
     1861 actggtgaga gccgttaccg cggtctttcg tggcgaagcc attttcagcc ccactatagc
     1921 gcagcgaatg atgaactact tctccaatgt ctcacgggaa ggcaggtcaa aagccgatac
     1981 tgccttccca gagcttacag agcgcgagct ggatatcctg gaactgatcg cacagggaca
     2041 tggcaacccg gcaattgcca ataaactctc gttgagtgta aaaactgtac agaattacgt
     2101 ctctaacatc ctgaacaaat tacaggttgt ggatcgctca gaagcgatcg tccgtgcccg
     2161 tgaaaaagga ttgggcgtgg ataagaaata aatgcaaatt agaccagaaa acaactgcgc
     2221 agctttggcg aagaaacaat ttttgaaatc aagtcgcctt tcctgtgcag tttcgtgcag
     2281 ttaggatgaa aattgcccaa ttttcctgat aaagacctaa ggatgtttat atcctgggtc
     2341 tttttttatt gccaccagcg cggacgtcag aaccatgttc aggaaggcgt cgtgaagatc
     2401 caactggctt aggagttatg gatctcatgt tccatttata cttttaactt tgctcgtttg
     2461 gtatgaaaaa ttggatatga attgatacaa aagatgccct catacatgtg gattgaaaag
     2521 tcagcttgat gaatcagctg aagactttaa gcagacattg ggctcggtag ttccggggaa
     2581 tggaatgcaa ccaatgatgg tagctcatct gtattcatgt tactatacgt gcagcctgtt
     2641 ctcccctcac gcaatagcca gtagataaaa ccaggaacag ggcatggaca gggtttgttg
     2701 agtacccctc aggtttcagt cgtttcttga ttatgcattt ctacgctgtt tctatcggtt
     2761 ctacagtccc tcagaataat ttcactgcgg cgattcaaag cgtctttgaa tcatcgctca
     2821 acttacgcct cggtcatcag gatcggttga ttacgatcct tgtttcggat cactatgatc
     2881 tgcctcaagg gattcggttg attgctagaa acgttccatt tcaatcactg gtggtgggtc
     2941 tgcgagccgc ttccacggag aaaattctac gctttgagtc ttctccgcta ctgatcagct
     3001 tgcgtgaagc ctcaatttgg gaagcacaaa taccctacat tgatgtgacc ctggcctcca
     3061 ccagacaagc ctgggcgagg ctatggcatt ctctcaattt acagcaaaga ctcaagggaa
     3121 ctgaactaat tggcgaagat ctttttcaaa cggagcaagg ttctcatctc acccgtaaac
     3181 tcagccagcc cgttttaggt cttattgcag ggacaaaaga ttatgatgtt gagacggctg
     3241 ttgacgcggc gcaaaagatg atcgggctgg gacctggcgt cacgcctacc ggagatgata
     3301 ttctgatcgg ttacctggct ggcttgtgga gcgcgacggg tgatgacaat caacagatca
     3361 aattcgttga agcatttggg aatgctctct tgaatctcat ccggcagacg aatgagatca
     3421 gtcgcacgta tctatatcat gcaattcaca agcaattctc gagcagcctt atcaagttac
     3481 taaatgccat tcgcgaaggt gaaccagacc agatacagac t
//
LOCUS       DASTJP010000843         4875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1349842, whole genome shotgun sequence.
ACCESSION   DASTJP010000843 DASTJP010000000
VERSION     DASTJP010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349842"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFQ23_24500"
     CDS             complement(<1..338)
                     /locus_tag="VFQ23_24500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909832.1"
                     /translation="MNIRCSFCQMPYTLSRIEMIDALQEMDAQKLSHYDAHCPRCKRA
                     TPIQRSRMEMMMPKWRDELKKLEVELKENPQIAAPESIASPEAEPVKQAESGPEPVPA
                     KAEIKGNTKA"
     gene            complement(365..1399)
                     /locus_tag="VFQ23_24505"
     CDS             complement(365..1399)
                     /locus_tag="VFQ23_24505"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909833.1"
                     /translation="MNTTPTREQILPLPVGYTARGGNIDDYKITFDLLNAHSQYMNGS
                     NDLNDPELIRLDWLNEGFNPETDLHMIFASDGSLAAFIECWLNQEPPAHPWIFGSVHP
                     EHWGRGIGGHIITWAEEHARLALEKCAPELRVAPRSGTEAHNANGNALFEGLGWKHIR
                     SYYRMVTDLDAAPGVPSFPDGITVRPYNPETETEEVYRTFVDSFKDHFGFMEQPFEKG
                     FAEFKHNLIEEPGYDPRLWFVAMDGNEMAGICLCRGEDMEDAESGWVNELGVRRAWRK
                     RGLGNALLKHAFATFHADGKKRASLAVDASSLTGALKLYEGAGMRVLRQFNQYEKEFR
                     PGKEISVQSL"
     gene            complement(1396..2304)
                     /locus_tag="VFQ23_24510"
     CDS             complement(1396..2304)
                     /locus_tag="VFQ23_24510"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909834.1"
                     /translation="MMRARGYEGMQDLYAMLDLLTEGSKAKNGAYYVHRGDLQWWLFY
                     TDVPEETWRSHIRLWFEDDKLIGWALLTLGEDVFDVYVSPHLRGDAQEQEMLAWASQE
                     MSAHGDVQNIWAAEDDDVRTRWLEENGFRPEEAHFIHFSRSLSGPLASPPLPEGFSIR
                     TARGPEDARLRSVASHAAFGSGKPFDEYWPRTLRFMQSAVYIPEHEIFVISPNGEVAA
                     FCIIWTDEHTKVGHFEPVATHPNFQRMGLGKSLLFESLRRLKSEGMNEADVFTNHDNP
                     AAIRLYESVGFQKSKRLFTYKKERTT"
     gene            complement(2301..2876)
                     /locus_tag="VFQ23_24515"
     CDS             complement(2301..2876)
                     /locus_tag="VFQ23_24515"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9909835.1"
                     /translation="MRELFRGELVRMTSEDPEARAKVEFQWQRDTEFHRLADANPARM
                     ESEKKIKEWVEKQNEDGFQPQRYSFSVRTLEEDKHIGFFVLWVDLIHKEAWVGIGIGD
                     REFWGKGYGTDMMKLCLQYAFMELNVQRVSLGLHEYNPRALRSYEKAGFRLEGRTRQD
                     LVREGKRYDSFWMGILRDEWIALQETEGANA"
     gene            complement(2886..3488)
                     /locus_tag="VFQ23_24520"
     CDS             complement(2886..3488)
                     /locus_tag="VFQ23_24520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9909836.1"
                     /translation="MFDIQTQLFEGRDIRFGPIDHEKDPEIESKWTHDSEFIRLMEVN
                     AARPMSAAILKKQYEKIEKQVDDDKNFFYFAIRAKEDDRLIGKALIQSVEWTNAYGWI
                     HLGIGSAENRRKGYGSQALTLLLRFAFAELNLFRVSARVPEYNEAGIALFTKFHFVEE
                     VRQRQALERDGRRWDILIFGLLISEWQNDTRPDLNANEGK"
     gene            3776..4213
                     /locus_tag="VFQ23_24525"
     CDS             3776..4213
                     /locus_tag="VFQ23_24525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909837.1"
                     /translation="MVAWKANADTIPLHTVLIVSDQPESVAVLDTLFNQRNCIVLSES
                     NISDALQSARLVGPSLMLIDMQLSKTERAALLKGLRAASRGPILMLISANTVDEIMEA
                     NQAGADECLVKPVNSAVLVIKAMAWLGHGQRSERSSAILGVTL"
     gene            complement(4300..>4875)
                     /locus_tag="VFQ23_24530"
     CDS             complement(4300..>4875)
                     /locus_tag="VFQ23_24530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909838.1"
                     /translation="QSDIAAFLAGYTAAMITDDYRIGMMIPKDNPDAIRSLNAFANGM
                     KAYCGICRPLFFYQSTFPQYIEIGAEEDPANYDAYADILMLQYKVGTIYLHPDIATTD
                     LMNYIGTTGASMIGTRTPEQQPAGWVMTIQPDVIKAIQNAWPSLINGQGGVTVQSPLG
                     LADIDPSLLTPGKQRLVEQTLNELQAGKIVP"
BASE COUNT         1208 a         1302 c         1119 g         1246 t
ORIGIN      
        1 ttggctttgg tattgccctt gatttcagct tttgccggaa ctggctcggg accagactca
       61 gcctgtttca caggttcagc ctcaggcgaa gcgatcgatt ctggcgcggc gatctgtgga
      121 ttttccttca actccacctc cagctttttc agttcatcgc gccactttgg catcatcatc
      181 tccatccgcg aacgttggat cggcgttgca cgcttgcaac gcgggcaatg agcatcatag
      241 tgactcaatt tttgcgcgtc catttcctgc aacgcatcga tcatttcaat gcggctaagt
      301 gtataaggca tctggcagaa agaacaacga atgttcatgg atactccttt attgcaatag
      361 tttttcagag ggattgtaca ctgatttctt taccaggtct gaattctttt tcatactggt
      421 tgaactgacg caggacgcgc atccccgcgc cttcatacag cttcagtgcg ccagtgagac
      481 tacttgcatc tacagcgagg ctagcgcgct tttttccatc cgcatgaaag gtagcgaagg
      541 catgcttgag cagagcattc ccaagcccgc gtttgcgcca cgcacggcgc acacccagtt
      601 catttaccca tccagactca gcatcctcca tatcttcacc gcggcaaaga cagataccag
      661 ccatttcatt gccatccata gcaacaaacc acagacgcgg gtcataacct ggttcctcaa
      721 ttaaattgtg tttgaactct gcaaaacctt tttcaaatgg ctgttccatg aaaccaaagt
      781 gatctttgaa tgaatcaaca aaggttcgat agacctcttc ggtctccgtt tcagggttgt
      841 atggacgcac ggtgatccca tcagggaaag aaggaacacc aggtgcggca tccaggtctg
      901 taaccatgcg atagtaagag cggatgtgtt tccaccctaa cccttcaaaa agcgcattcc
      961 cgttagcgtt gtgtgcttca gtaccagaac ggggcgccac tcgcaattcg ggagcacatt
     1021 tttcgagtgc aaggcgggcg tgctcttctg cccaggtaat gatgtgaccg ccaatacccc
     1081 taccccaatg ctcaggatgt acactgccaa agatccacgg atgagccggc ggttcctgat
     1141 tgagccagca ttcgataaac gcggcgagag agccatcgga cgcaaagatc atgtgtagat
     1201 cggtctcagg gttgaaaccc tcattcagcc aatccagccg aatcaattca gggtcattca
     1261 ggtcattgct tccattcatg tactgtgagt gagcattgag caaatcgaaa gtgattttgt
     1321 aatcatcaat gtttccaccg cgcgccgtat agcccacggg taatggaaga atttgttctc
     1381 tggtaggtgt tgtattcatg ttgttctctc tttcttatag gtaaataaac gtttggattt
     1441 ttgaaaccca accgactcat atagacggat agcagccggg ttatcatgat tcgtgaagac
     1501 atctgcttcg ttcatccctt cagacttgag cctgcgcagg ctttcgaaca gcagactctt
     1561 tcctaaaccc attctctgaa aatttgggtg tgtagcaacg ggctcgaaat gcccaacctt
     1621 cgtgtgttca tctgtccaga tgatgcaaaa tgcagcgact tctccgtttg gagagatgac
     1681 aaaaatctca tgttcgggga tatatacagc tgactgcatg aaccgcaagg tacgcggcca
     1741 atactcatca aatggcttgc ccgatccaaa tgctgcatga gacgcaaccg aacgtagacg
     1801 agcgtcctcc ggacctcgcg cggtacggat gctgaatcct tctggcagcg gcggactggc
     1861 caagggtcca gatagagatc ggctgaaatg aatgaaatgt gcttcttcgg gcctaaagcc
     1921 attttcctcc agccagcgag tgcgaacgtc atcgtcctcc gcagcccaaa tattttgtac
     1981 gtcgccatgc gcggacattt cctgggaagc ccatgcaagc atttcctgct cttgagcgtc
     2041 gccgcgcaaa tgcggagaaa cataaacatc gaagacatct tcgcccagcg taagcaacgc
     2101 ccagccaatc agcttgtcat cctcaaacca caaacggatg tgagaccgcc aggtttcctc
     2161 aggtacatcg gtgtaaaaaa gccaccactg caagtcaccg cggtgaacat aatatgcgcc
     2221 gtttttagct ttgctccctt ctgtgagtaa atcaagcatg gcgtataagt cctgcattcc
     2281 ttcgtaaccg cgtgcgcgca tcatgcattc gccccttctg tctcttgcag agcaatccac
     2341 tcatcgcgca ggatacccat ccaaaacgag tcgtatcgtt tgccttcgcg caccagatcc
     2401 tggcgcgttc gaccctccag gcgaaaccct gccttctcat aagaacgcag ggcacgcggg
     2461 ttgtattcat gcaaacccag ggaaacacgc tgcacattca attccatgaa tgcatactga
     2521 agacacaatt tcatcatatc ggtcccatag cccttgcccc agaattcgcg atcaccgatg
     2581 ccaattccta cccaggcttc cttatggatc aggtccaccc acaggacaaa gaagccgata
     2641 tgtttatctt cttccagtgt acgaacagaa aaggagtaac gctgaggctg gaaaccatcc
     2701 tcattttgtt tttcaaccca ttcctttatt ttcttttctg attccattcg ggcagggtta
     2761 gcgtccgcca agcggtgaaa ttccgtgtcg cgctgccact ggaattcgac tttggcgcgt
     2821 gcctcgggat cctcagacgt catgcggacg agctcgccgc gaaataattc tctcatcggt
     2881 tcctcctact ttccttcgtt tgcattcaga tcaggtcttg tatcattctg ccactcagaa
     2941 attaacaagc caaagatgag gatgtcccat cggcggccgt cgcgttcaag tgcctgccgc
     3001 tgacggacct cctccacaaa atgaaatttt gtaaacaagg cgattcctgc ttcgttgtat
     3061 tcaggcaccc gtgcgcttac ccgaaagaga tttaattctg caaacgcaaa ccgcagaagc
     3121 agcgtcagag cttgtgagcc ataccccttg cgccggtttt ctgcagaccc aatgccgaga
     3181 tgaatccacc cataggcatt tgtccactcc accgactgaa tcagggcttt accgatcaac
     3241 ctgtcatctt ctttggcacg gatcgcaaaa taaaagaagt tcttatcatc atcgacttgc
     3301 ttctcaatct tttcatattg ctttttgaga atggcggcag acatgggacg cgcggcgttg
     3361 acttccatta gacgtatgaa ctcagaatca tgcgtccatt tggattcgat ctcgggatcc
     3421 ttttcgtggt cgattggacc gaagcgaata tccctgcctt caaataattg agtttgaatg
     3481 tcgaacatgt tacctccagg aaaataaaaa aatagccaca ggcatttgcc catagcttca
     3541 ccaggaaaaa aagaagccac gggcgagtgc accgtggctg taatctacat tattgcgtgt
     3601 aaattaacga gtaacaggcg cactccgagg atgcgaaaag tccccaccca caacatttcg
     3661 actttggatc aaattactaa acatcggagc gcctcctttc tttaaagttg ttatattttg
     3721 agtgtaacat gttgacttga ttcacgtcaa gttaacaaca aagaaaggaa aacccatggt
     3781 tgcctggaag gctaatgccg atacaatccc cctccacaca gtgttgatcg tctctgatca
     3841 gcccgagagc gtcgcagttt tggacacgct ttttaaccag agaaattgta tcgtcctgtc
     3901 tgaatccaat atctcggacg ccttacagag cgcgcggctg gtgggtcctt ccttaatgtt
     3961 gatagacatg caattatcca agactgagcg cgcggctctt ttgaaaggtt tacgcgcagc
     4021 cagccgtggt ccgatcttga tgctaatctc cgcgaacact gttgatgaaa tcatggaagc
     4081 caaccaggcg ggcgccgatg aatgccttgt aaagcctgtc aactctgccg tactcgttat
     4141 caaagccatg gcatggcttg gacacggtca acgaagcgaa agatcttctg cgatcctggg
     4201 ggtgacttta taagtccccc tttgctattc tccttcttct cctccttttt gatgccaaaa
     4261 ctctcgtgac cttcacggga gttttggttt aaggaagctt tagggaacga tcttacccgc
     4321 ctgcaattca tttaatgttt gctcaacaag acgttgcttc cccggagtaa gcaggcttgg
     4381 gtctatatcg gcaagcccca ggggagactg aaccgtgacc ccgccttgcc cattaatcaa
     4441 actaggccag gcattctgaa tagccttgat cacgtccggc tgaatggtca tcacccagcc
     4501 cgcgggttgc tgctcgggcg tcctggttcc gatcatggac gcgccagttg tgccaatata
     4561 gttcattaaa tctgtcgtcg cgatatcagg atgaaggtag atggtaccga ctttgtattg
     4621 cagcatcagg atatctgcat aggcgtcata attggccgga tcttcctccg cgccgatctc
     4681 aatatactgc gggaaagtcg attgataaaa aaagagcgga cggcaaatcc cgcaataagc
     4741 tttcataccg ttcgcaaaag cattcaacga acggatggca tctggattgt ccttcggaat
     4801 catcatgccg atacgataat cgtcggtgat catagcagcc gtatagccgg ccaggaatgc
     4861 tgcaatgtca gattg
//
LOCUS       DASTJP010000844         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_926430, whole genome shotgun sequence.
ACCESSION   DASTJP010000844 DASTJP010000000
VERSION     DASTJP010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926430"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1086)
                     /locus_tag="VFQ23_24535"
     CDS             complement(226..1086)
                     /locus_tag="VFQ23_24535"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET9909839.1"
                     /translation="MNVQVILVPYDSGQRSIRMGSGPEHFINNGLAQALEANGHKVSV
                     ETIETPTEFRAEVQTQFQLYRSLAKKVAEAKQNSRFPLVLSGNCGATLGVIAGSGAKQ
                     PGVIWFDAHGEFNTPETTTSGFLDGMGLAIATGTCWKKLAASIPNFQPLPAEHILLVG
                     GRDFDEGEKEGLEQAGASVVDAVTISQRGIRDALQSAITGLQSNVEEVHLHIDLDALN
                     PKEVPSNGFVVEDSGLSLNEMSEAIQYVKESLKIRSLTIASFDPSYDHEGKALQASLN
                     LIQQVIGDQS"
     gene            complement(1120..1845)
                     /locus_tag="VFQ23_24540"
     CDS             complement(1120..1845)
                     /locus_tag="VFQ23_24540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909840.1"
                     /translation="MLTDELLNYLFDGRNHILTGDMRAWLGASRRFTTFVASFRDKIR
                     KKLRVTQDRETLYDLRLELETAYLLLNERTLNVVYEPEQSGPGRSPDFAVTYTTSLTF
                     MVEVTRLRSIAVLDHGLPLESDRFADAICSKLRQFLPQRSNILIVGVDAMYLTQSELQ
                     SIMLRIQQRAEQNDSSYWQRYGFRERTDFFKGYQRLSEIIVRSSQLQADEAPIVWVNS
                     QANHSLPSKVRTVLYRSHTASSH"
     gene            complement(1879..>2516)
                     /locus_tag="VFQ23_24545"
     CDS             complement(1879..>2516)
                     /locus_tag="VFQ23_24545"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9909841.1"
                     /translation="NLVLQITEDQRPWCPEFDGDVKASCIQTGLFAGPLGSRLGQLRF
                     SDELIVREAQTNARLYTPQYGYFECRAKAINIPGNHVSLYMIGYEDTPEKCGEINLFE
                     IFGKNVGPNSANVGYGIHPWKDPKLIDEFYQDWLGIDATKFHVYALEWTPSHIDFYVD
                     TQKIRWIHQSPDYPMQFILSIYELPGGDINAPYPKEFVIDYVRAYQPETGY"
BASE COUNT          611 a          645 c          596 g          664 t
ORIGIN      
        1 tcaaagagct cgagtgtggc tttgcccata tccaggatca gtgcctggcc gccatcattg
       61 ttccagatgg cagcgggttc tatgccaaga ccttcgcaat agaatttgac taaccgctca
      121 tagtctctgg ctgtcagtgc gacacggaac tcaaggatgg gaggattaag gttcgacatg
      181 aaaaggatgg cctttctgga tttctacgca atggcatagc aagaattaag attgatcgcc
      241 tattacctgc tgtatcaggt tcagactagc ctgtaatgct tttccttcat ggtcataact
      301 ggggtcgaaa gaggcgatcg ttaatgagcg gatctttaag ctttccttca catattggat
      361 tgcctcgctc atctcattta ggcttagccc actatcttcc accacaaatc catttgaggg
      421 aacttccttc ggatttagcg catccaggtc gatgtgcaaa tggacttctt caacattgct
      481 ctgaagaccc gtgattgcag attgtagggc atcccgaata ccgcgttgtg aaattgtgac
      541 tgcgtctaca acactggcgc ctgcctgttc gagcccttct ttttctcctt catcgaagtc
      601 acgaccgcca acaagcaaga tgtgctcagc aggcagtggt tgaaaattag ggatcgaagc
      661 ggctaatttc ttccaacagg ttcctgtcgc gatcgccaac cccataccgt ccagaaatcc
      721 gcttgttgtg gtttccggtg tattgaactc accatgcgca tcgaaccaaa tcacgccagg
      781 ctgctttgca ccggagcctg caattacgcc taaggtcgcg ccgcaattgc cagagagaac
      841 cagcggaaat ctactgttct gttttgcttc ggcaaccttt ttcgcaagcg atctatataa
      901 ttggaattgt gtttggactt ctgctcggaa ctccgttggc gtctcgatgg tttccaccga
      961 aaccttgtga ccattggctt ccaaagcttg cgcgagcccg ttgttgataa aatgctctgg
     1021 cccgctgccc atgcgaatac tgcgctgccc cgagtcatac gggactagga tgacttgcac
     1081 gttcattttt cctctgcctc cttttacttc cgaatactac taatgcgatg aagctgtgtg
     1141 acttcgatac aagaccgttc gcaccttgct cggtaatgaa tgattcgctt gagaattcac
     1201 ccatacgatc ggggcttcat ctgcctgcaa ttgcgaactg cgcacgatga tttcgcttaa
     1261 tcgctgataa cctttaaaaa agtcagttcg ttctcgaaag ccatatcgct gccaatacga
     1321 agagtcattc tgttcggcgc gctgttggat acgcagcatg atggattgaa gctcgctttg
     1381 cgtcagatac atggcatcca caccgacaat gaggatgttg ctgcgctgcg ggaggaattg
     1441 acgcagtttg ctgcagatcg catcggcgaa acggtcgctc tccagtggaa gaccgtgatc
     1501 taaaaccgcg atgctccgca accgtgtgac ttcgaccata aaggtgagac tggtcgtata
     1561 ggtgacggca aaatcaggac tgcgtcccgg cccggattgc tctggctcat agactacatt
     1621 gagagtccgt tcgttgagca acagataagc tgtctccagc tcgagccgca aatcgtataa
     1681 agtttcccga tcctgggtca cgcgtagttt cttgcggatc ttatcccgaa aagaagcgac
     1741 aaacgtggtg aaccgccgcg atgctcccag ccaagcgcgc atgtccccgg taaggatatg
     1801 atttcgccca tcgaaaagat agttgagtaa ttcgtcggtc agcatggatg cacaagttta
     1861 acataaggtg cccacaggtt aataccccgt ctccggctga taagctctga cataatcgat
     1921 tacaaactct tttggataag gggcgtttat atcgccgcct ggcagctcat agatcgataa
     1981 tataaattgc atcggatagt caggcgattg gtggatccat ctgatcttct gagtatctac
     2041 ataaaagtca atatgagatg gcgtccactc aagtgcgtag acgtgaaact tcgttgcatc
     2101 gatacccaac caatcctggt aaaactcatc gatcagtttc gggtccttcc aaggatgaat
     2161 gccatatcct acatttgcag aattgggccc gacattcttg ccaaaaatct cgaacaggtt
     2221 gatctcgcca cacttttccg gcgtatcttc atacccgatc atatacaacg aaacatgatt
     2281 tcccgggatg ttgatagctt tcgctctgca ttcaaaataa ccgtactgag gtgtatagag
     2341 tctggcattg gtctgtgctt ctctgacaat taactcatca ctgaagcgaa gctgccccag
     2401 tcgactccca aggggacccg caaataaacc ggtctggatg catgaagctt tcacatctcc
     2461 atcgaattcg gggcaccacg ggcgttggtc ttcagtgatc tgcagtacca ggttgc
//
LOCUS       DASTJP010000845         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_960343, whole genome shotgun sequence.
ACCESSION   DASTJP010000845 DASTJP010000000
VERSION     DASTJP010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960343"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2144
                     /locus_tag="VFQ23_24550"
     CDS             <1..2144
                     /locus_tag="VFQ23_24550"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9909842.1"
                     /translation="GTVEFLLTSHPLDCPICDKGGECPLQNLTMRHGPGKSRFLFDEK
                     LRLGKRVPLGELIVLDQERCIQCARCVRFQEEVVGEPVIEFAERGRAMQIVTYSDPGF
                     DSVFSGNTTDICPVGALTTVDFRFEARPWELGEVASVCTQCPVGCNLTLNVRREAKAG
                     GDFVVKRVMPRQNEDVNEIWICDKGRFAHHYTDSKKRLTKPLVRNADKLTHASWESAT
                     KQAADHFAAAKKDFVVLASGRLANEDLFNLKSLADRAGAKAYLYSYIGGGELTSLIGV
                     GEGTNFGSMGKGTTIVVVASDLYEEAPIWYLRVKQAAERGANVIVLNPRETKLERFAT
                     HALRYDYGDEVKTILDLGKKDGKAGEGFTSAENAVILFGSEGLGIAGTTGLASACANL
                     LRDHGFVGKPNNGLIGVWERANDQGAWEMGFQVDEDLANALKGKTVYIVGANPAADDP
                     KLAKALEGAEFVVVQDVMETVTTEMADVVLPAQTFTEREGTLTSGERRVQRFYPAVPP
                     TGDSKPDFSITSQIARHMGIDLEGTSVTAVFDTLANSVNSYEGLNYARLAEVKPQWPV
                     VHRGDLYYGGTSYENRHGLGAHLTAAAARGETVSIPPFQGESAPHPKDKELLAVPITK
                     LYDQGTTVMPSGLLHGRIGETRVGLHPQTAKRLGVEEGQTVNVSFNGTSGDAIVRLDD
                     SIADGVALVPRSMGIAIREPVPVNVRSQVKS"
     gene            2141..>2786
                     /locus_tag="VFQ23_24555"
     CDS             2141..>2786
                     /locus_tag="VFQ23_24555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HET9909843.1"
                     /translation="MITDWTLWLEWFIKSVILILALLGGFAYLTWYERRALARIQTRV
                     GPNRAGPFGLLQPIADAVKLIFKEELTPAKAFKPVFFLAPILTVVPSIIIAAVIPWGR
                     SISLFGREITLYLADINVGILYFMSIAAISVYGIVLAGWSSNSKYAMLGGLRATAQML
                     SYELSLGISLVPVILLANSMSMIEIVDAQNPFWFILIQPVGAFIFWIATLAEVNR"
BASE COUNT          651 a          669 c          792 g          674 t
ORIGIN      
        1 gaggaacggt tgaatttctg ctcacctctc atccgctgga ctgcccgatc tgcgataaag
       61 gcggcgaatg tccactgcaa aatctcacca tgaggcacgg accgggcaaa agccgcttcc
      121 tctttgatga aaaacttcgt ctgggaaaac gcgtgccgct tggtgaacta attgtcctcg
      181 accaggaacg atgtatccaa tgtgcgcgtt gtgttcgatt tcaggaggaa gtcgtcggtg
      241 aaccggtaat cgagtttgcg gaacgcggcc gagccatgca gatcgtcacc tattcagacc
      301 ccggctttga ttccgttttc tcaggcaaca ccaccgatat ctgcccggtc ggcgcgttaa
      361 cgactgtgga cttccgcttc gaggcgcgcc catgggaact gggagaggtt gcttcggtat
      421 gcacccaatg cccggtgggc tgcaatctga cattgaacgt gaggcgtgaa gcaaaagcgg
      481 gtggtgactt cgtcgtgaag cgcgtaatgc cgcgccagaa cgaagacgtg aatgaaatct
      541 ggatctgtga taaaggtcgt tttgcacatc actacactga cagcaaaaaa cgactgacaa
      601 agcctcttgt ccgtaacgct gataaactga ctcacgcttc ctgggaatct gcaacgaaac
      661 aggcggcaga tcatttcgcg gctgctaaga aagattttgt cgttcttgcc tccggtagat
      721 tggcgaatga agacttgttc aatctgaagt ctcttgctga tcgcgcgggt gcgaaggctt
      781 atctctattc ctatatcggt ggaggcgagc tgacttcgct gatcggtgtg ggcgaaggca
      841 caaattttgg aagtatgggc aaaggcacaa ccattgtggt cgttgcctct gatctctatg
      901 aagaagcgcc gatctggtat ttgcgagtga aacaggcggc tgaacgtggc gcgaacgtga
      961 tcgtgttgaa tccacgtgag acgaagctcg aacgctttgc tacacatgca ctgcgttatg
     1021 actatgggga tgaagtaaag acaattcttg accttggtaa aaaagacggc aaggctggcg
     1081 aaggcttcac cagcgccgag aacgcggtca ttctttttgg aagcgaaggg ctgggcatcg
     1141 ccgggaccac tggtttagct tctgcttgtg ccaacctgtt gagggatcat ggctttgtcg
     1201 gcaaacccaa taacggactg atcggcgtct gggaacgtgc caacgatcag ggcgcctggg
     1261 aaatgggttt ccaggtggac gaagatctgg cgaatgcact aaaaggcaaa acagtttaca
     1321 tcgtcggtgc gaaccctgcg gcggatgacc cgaaattggc caaagcgctg gaaggtgcag
     1381 agtttgttgt cgtgcaggat gtgatggaaa ctgtgaccac cgaaatggcg gatgtggttc
     1441 ttccggcgca gacgtttaca gaacgcgaag gaacgcttac ctccggtgag cgacgcgtgc
     1501 aacggttcta tccggcagtg cctccgaccg gagactcaaa accagatttt tcgatcacat
     1561 cgcagatcgc gcgacacatg ggcattgacc tggaaggaac gtctgtcact gccgtgtttg
     1621 atactctggc aaattcagtg aattcctatg agggtttgaa ttacgcccgg ctggcggaag
     1681 tcaagccaca gtggccagtc gtccatcgtg gagatctgta ttacggcgga accagctatg
     1741 agaacaggca tggcctgggc gcgcatctca cagctgcggc agcgcgcgga gaaacagtga
     1801 gcattcctcc gttccaggga gagtcggctc cacatccgaa agataaagaa ttactggctg
     1861 tacccatcac gaaactctat gaccaaggca cgacggtgat gcctagtgga ttgctgcatg
     1921 ggcgcatcgg tgaaactcgc gttgggttac atcctcaaac cgcaaagaga ctcggcgttg
     1981 aagaaggtca gaccgtcaat gtcagtttca atggcacgag cggcgatgcg attgtgagac
     2041 ttgacgactc gattgcagat ggcgtggcat tggtcccgcg cagtatggga attgcaattc
     2101 gcgaaccggt tccggtaaac gtacgttcac aggtgaaatc atgataacgg actggactct
     2161 gtggctcgaa tggtttatca agtcggtgat cttgatcttg gcattgctgg gtggttttgc
     2221 ctatctcacc tggtacgaac gccgcgcgct ggcgcgcatc cagacccgtg taggacccaa
     2281 cagagccgga ccttttggtt tgcttcagcc gattgcagat gccgtaaagc tgatctttaa
     2341 agaggaacta acaccagcca aagcattcaa gcctgtcttt tttctggcac cgattctgac
     2401 cgttgtacct tcgatcatca ttgcagcagt cattccatgg ggacgctcga tcagtttgtt
     2461 cgggcgcgag atcacgttat atctggcaga tatcaatgta ggcattttat atttcatgtc
     2521 catcgctgcg atttcagtct atgggattgt gctggcgggc tggtcatcca atagtaaata
     2581 tgccatgctg ggtggtttgc gtgctaccgc acagatgctt tcgtatgagt tatctctggg
     2641 tatttcgctt gtgcctgtta ttttgctggc aaattcaatg agtatgattg agattgtaga
     2701 tgcgcaaaac cccttctggt ttatcctcat tcaaccagtg ggagccttca tcttttggat
     2761 tgcgactctt gcagaggtaa accggg
//
LOCUS       DASTJP010000846         6766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_926458, whole genome shotgun sequence.
ACCESSION   DASTJP010000846 DASTJP010000000
VERSION     DASTJP010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926458"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2490
                     /locus_tag="VFQ23_24560"
     CDS             <1..2490
                     /locus_tag="VFQ23_24560"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HET9909844.1"
                     /translation="NIDKTAPTLNLPSNITVEATSLAGAVVNYIASVSDNLDPAPSIS
                     CLPTSGNTFVIGTTMVNCSANDHADNTAFGNFNITVQDSAAPIIAFHADVTAEATSAS
                     GANVSYTSPSTSDAVDGSGTATCSPASGSLFALGDTTITCSATDSNGNAAIPTTFVIH
                     VVDTTAPVIAAHADITVEATSASGAVVTYTNPTTTDAVDGAGTSFCSPSSGETFSLGN
                     TTVTCTAADSHGNVSTPATFVVHVVDTTAPLIASHADVTVEATSSAGAAVTYASPTTS
                     DIVDGAGTATCSPASGTTFAIGNTTVTCNATDSNGNAAVATTFVVHVVDTTAPVINPH
                     ADVFVPATSALGAIVNYSTPTTTDAVDGAGVANCTPASGSQFAIGNTTVTCNATDSHG
                     NAAVPTSFVVHVVDTAAPVIDGHLDVTVEATSAAGAIATYTSPTTSDVVDGPGVATCS
                     PGSGTFFVFGDTTVTCTATDSNGNAATPTTFIVHVVDTTAPSIASHADVTAEATSASG
                     ASVTYTNPTTSDIVDGTGVASCSPASGSTFALGNTTVTCTATDSHGNSASSTSFVVHV
                     VDSTAPVISAQADISVFIDNLIGTIVSYSAPTTSDAVDGPGTASCSPFSGTFFSLGDT
                     LITCNASDSQGNAAQPVTFTVHVVYQPGAPAPTTETTRNPQSGLSIPVTSGLLDLDCL
                     TVINSFGVQVTFHNLCDYQAAVTEAPADTLPAPLPEEYSFVQGLNVLVLFEEEVVKAL
                     PTGTGVQLDFSIPADTQDEYDVLLWDDKDGDGIGEWLKVTRLVLDRDLSKVMLADPSD
                     ELYQIARTETLDAFYRIITTEKTGTFILVKN"
     gene            2566..3087
                     /locus_tag="VFQ23_24565"
     CDS             2566..3087
                     /locus_tag="VFQ23_24565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909845.1"
                     /translation="MKSRFLIVLIPCLLLLIACANQTLTITPSSPTTDQTLTSTFTAT
                     PDPCSVEHLSESIKVVNDLMREFDDMSKLAANLAREKLPELISEMQRVRRAAEDQNTP
                     ACLSTLKTHQISHMNIVIDTLIAFLGGAQPETLNDGIARAGQAHDLYTIEVARLLGIP
                     QGITSTPAVTITP"
     gene            3176..3994
                     /locus_tag="VFQ23_24570"
     CDS             3176..3994
                     /locus_tag="VFQ23_24570"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HET9909846.1"
                     /translation="MTTKTYENDLLQWRAKKYDERVRENGWLALAGLFWLKQGRNLIG
                     SSSVCEVVLPERAPTFIGIAELKRDTVRVQVTEGVPVKINDRFVQKAILKSNKDEKPS
                     FITWNDIRLVLHEYAGRYAMRVWDNKREQRFNFPPLKWFPIDKKFHISARYTRYPSPK
                     ISPQEDTFGGTVEDRLDGYITFKFDGTTYKLDATETDDQKLFVKFRDATSGKETYPAS
                     RYLYTLPVNNNKVVLDFNYAYSPPCAFTEYATCLFASPQNNLPFRVEAGEMYRG"
     gene            complement(4058..4936)
                     /locus_tag="VFQ23_24575"
     CDS             complement(4058..4936)
                     /locus_tag="VFQ23_24575"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET9909847.1"
                     /translation="MLVTWKYRPRNTFIQRLDPRTRFILLLCTILALTIPEIWDYRIL
                     VPLFLFSLTQFLMARIEWRDIRRPLFFIFILVFFIVGLNGLLSGRGGPSSVNDPNSPI
                     LFQALIPGTTWGLTVTISKLWFAMSQIIRILTMALPAILIPYTIDPNIYGTTFKQLKA
                     SDKVAFTIDLAFRFLPTFARDLTITMDAQRARGYEMDKLKGGVAAKISKLAPLVIPVT
                     MQSTVTGEEVIDAMDLRAFGTNPRTWLKKLEYQSRDYFVISLGVAIFLACCILKWGFG
                     IGDFFVPDFFTKALLG"
     gene            complement(4942..5490)
                     /locus_tag="VFQ23_24580"
     CDS             complement(4942..5490)
                     /locus_tag="VFQ23_24580"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET9909848.1"
                     /translation="MNNNSTWEFGTRQVVYGAIGAALYGALSWVSNFIPLPAAGNITF
                     RPAVAVLIFFGVAYGPIVGFLAGFIGNTLGDLISGFGFYWNWSLGNGLMGLVPGLIMA
                     AITDFKDRADIIKAVGWGALGVAVGMLFASLTEMFVGGIDLSTALVGYFTPAFIGNLV
                     VVAILLPILMIAFAGVAARRGR"
     gene            complement(5624..>6766)
                     /locus_tag="VFQ23_24585"
     CDS             complement(5624..>6766)
                     /locus_tag="VFQ23_24585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9909849.1"
                     /translation="LLLDEPLASLDPASAHEALELFRSLADEGKTIVLIEHRVEDAIV
                     AKPDRLLYMEAGQIKYLGPIDALPTEINHHEVKLPAEWVVKRVRQLGEKIEKTEPAKN
                     SERGEPLIVFEDVDFRYDEELPLILQNVNLQIYPGDLVAILGPNGAGKSTLVKQTIGL
                     LKPTRGRVLMDGNDTRTMSVAQIARTLGFVFQSPTHMLYAPTVREELEFGPKNLEFNP
                     VAIKRSVAESVSTVNLKGFEEYPPLGLSFGQQKRTTIAAVLAMQSKIMIMDEPTAGQD
                     YANYTHFMDAMCRPVNGAKSLVAANFAATIFITHDLDLAITYANRVLLFGDKHVVADG
                     PPEEVLKDFDLLTRYRVRPTSLLRMNLDLLPRTKQFLTAEALAAYA"
BASE COUNT         1731 a         1841 c         1627 g         1567 t
ORIGIN      
        1 aatatcgata agacagcacc cactctcaat ttgccctcaa acattactgt tgaagcgacc
       61 agtctggcag gagcagttgt caattacata gcctccgtca gtgacaacct ggatcccgcg
      121 ccaagcatta gctgtctacc gacttctggg aacacctttg tcattgggac cacgatggta
      181 aactgctctg cgaacgacca tgcagataac acagcctttg gtaactttaa tatcacagtg
      241 caagactcag cagctccaat cattgctttt catgcagatg taacagctga agccacttct
      301 gcttctgggg cgaatgtgag ctatacaagc ccaagtacga gcgatgccgt ggatggttct
      361 ggaactgcaa catgctcacc agcctccgga agtctctttg cccttggcga tacaacgatt
      421 acttgtagtg ctacggactc caatggtaat gcagctatac cgacgacatt cgttatccat
      481 gtcgttgaca cgaccgctcc ggtaattgcg gcccatgccg atattacagt agaagctact
      541 tccgcttcag gtgcggtcgt cacgtacacc aatccaacca ccacagacgc ggtcgatggg
      601 gccgggacgt ccttctgttc cccctcttct ggcgaaacct tctctctcgg taacacaact
      661 gtcacatgca ccgcagccga ctctcacggc aatgtctcca cgccagctac ctttgtcgta
      721 catgtcgtgg ataccacagc acccctcatt gcatctcatg cagacgttac ggtcgaagca
      781 acctcatcag cgggcgcagc cgtcacgtac gcaagcccaa ccacatcgga tatcgtagat
      841 ggtgcaggaa ccgcaacctg ctcaccggct tcaggcacca ctttcgcgat tggcaataca
      901 actgtcacat gcaatgcgac agactcaaat ggcaatgcag ccgtcgcaac tacttttgtt
      961 gtgcatgtgg tcgatacaac ggctccagtc atcaatcctc acgcagatgt ctttgttcca
     1021 gcaacctctg cattgggcgc cattgtgaat tacagcaccc ctacgactac ggacgccgtg
     1081 gatggcgccg gggttgcaaa ctgtacacct gcttcaggca gtcaatttgc tattggaaac
     1141 acgaccgtca catgcaacgc cacagactct catggcaacg ccgctgtacc gacttcattt
     1201 gtcgtgcatg ttgttgacac agcagcaccg gtgattgacg gtcatctgga tgtaaccgtc
     1261 gaggcgactt ctgctgcagg cgcgatcgcc acctacacca gcccaactac ctcagacgtc
     1321 gtggatggtc cgggcgtggc gacctgcagc cccgggtcgg gaacgttctt cgtattcggc
     1381 gatacaacag ttacttgtac tgctactgac tccaatggca atgcagccac gcccacgacc
     1441 ttcatcgttc atgtagtgga taccacagcc ccgagcattg catctcatgc agatgtcact
     1501 gcggaagcca catcggcttc aggcgcatcc gtgacctata ccaatccaac gacatcagat
     1561 atcgtggatg gcactggtgt cgcatcttgt tctcccgctt cgggtagtac cttcgctctt
     1621 gggaacacaa ccgtgacatg cactgcaacg gactcacatg gcaactcagc ctcttcgaca
     1681 tcgtttgtcg tccatgtagt agatagtacg gcacctgtaa tttctgcaca agcagacata
     1741 tcagtcttca tagataacct cattggtacg atcgtgagtt attcagcgcc aaccacatca
     1801 gacgccgtag atggccccgg aaccgccagc tgttcgccgt tctctggaac tttcttctcc
     1861 ctaggcgata cgctcattac gtgtaacgcc agcgactcac agggcaatgc agctcagcct
     1921 gttacattca ccgttcatgt cgtgtatcag cccggcgcac cggctcccac taccgagaca
     1981 acgaggaatc cacaaagtgg actcagcatt ccggttacga gcggtttgct ggacctggac
     2041 tgcctgaccg tgatcaattc ctttggagtt caggttacct tccataacct gtgtgattat
     2101 caggcagccg ttaccgaagc acctgctgat acattgcctg ccccattacc ggaggagtat
     2161 tccttcgtgc agggattaaa tgtgctggtg ctgtttgaag aggaagttgt gaaggcacta
     2221 cccactggaa caggcgtaca attagacttc tccatcccag cagacacgca ggatgagtac
     2281 gatgtccttt tgtgggatga taaagacggt gacggaattg gcgaatggct taaggttacc
     2341 cggctcgttc ttgaccggga cctttccaaa gtgatgctgg ctgacccgag cgatgagtta
     2401 tatcaaattg cacgtaccga aacacttgac gctttctatc ggatcatcac caccgaaaag
     2461 accggcacct tcatcctcgt caagaattaa acatacgctt ctaagtcccc gatttgttga
     2521 gggacggtga acaccgtccc tcaactgttt ttttaggcac acaacatgaa atcgagattt
     2581 ctcattgtat taataccttg cttgttatta ctgatcgcct gcgcgaatca gactttgacc
     2641 attacaccct ccagcccgac gaccgaccaa actttaactt ctacgtttac cgctacaccc
     2701 gatccctgct cggtagagca cctgagtgag tccattaaag tcgtaaacga tctgatgcgt
     2761 gagttcgatg atatgtcaaa actggcggca aatcttgcaa gagaaaaact tcctgaactg
     2821 atttccgaaa tgcaacgtgt tcgccgcgcc gcggaagatc agaatacccc agcctgcctt
     2881 tccaccttga aaacacatca gatttcacac atgaacatcg tcatcgatac gctgatcgct
     2941 tttctcggcg gcgcacagcc agaaacactc aacgatggga tcgcacgtgc cggacaagcc
     3001 catgatctct atacgatcga agttgcgcgc ttattgggaa ttcctcaagg tatcacttca
     3061 acaccggcag tgacgatcac tccataaatc agattaaagt gaaactgtgc aatccacaag
     3121 agatatgcgt taatgcatgt ctcttgtttt acgtatggaa gtataatagc gtctcatgac
     3181 aacgaaaaca tacgagaatg acctcctgca gtggcgagca aagaaatacg atgaacgcgt
     3241 acgagaaaat ggatggttgg cactcgcagg tttattttgg ctaaaacagg gccgaaattt
     3301 aatcggctca agctccgttt gtgaagtcgt cctgccggag cgcgctccta cgtttattgg
     3361 catcgccgaa ctcaagcgcg acacggtccg cgtgcaagtg accgaaggtg ttcccgtcaa
     3421 aattaatgac cggttcgtac aaaaggcaat cctcaagtca aacaaggatg agaagcccag
     3481 cttcatcacc tggaacgaca ttcggttggt gctccatgaa tacgccggca gatatgcaat
     3541 gcgtgtttgg gacaacaaac gcgagcagcg ctttaatttt ccgcccttaa agtggttccc
     3601 aatcgataag aaatttcaca tttctgcccg ctatacgcgc tacccctccc ctaaaatctc
     3661 gccacaggaa gatacctttg gagggaccgt tgaagatcgc ttggacggat atattacgtt
     3721 caaattcgat ggaacaacgt acaagctgga tgccactgaa accgatgatc aaaaactgtt
     3781 tgtcaaattt cgtgatgcta ccagcggcaa ggaaacgtat cccgcgagca ggtatcttta
     3841 cacgctcccg gtgaacaata ataaggtcgt cttggatttc aactacgctt atagcccacc
     3901 ctgcgcgttc accgagtatg caacctgtct gtttgcttcg ccgcaaaaca atctgccttt
     3961 ccgcgtggag gcgggcgaga tgtatcgtgg ataattcaac acaaacgaaa aaaagtcgac
     4021 gaatcgtcgg ctttttttta ttattaatga atcgaggcta acccaatagt gcttttgtga
     4081 agaaatctgg cacgaagaaa tcgccgatcc caaaccccca cttcaaaata cagcaagcaa
     4141 ggaagatcgc tacccccaga cttatgacaa agtaatctcg tgactggtat tcgagttttt
     4201 tcaaccatgt gcgcggattc gtaccaaagg cgcgcaaatc catcgcgtcg atcacttctt
     4261 caccggtgac ggttgattgc atggtgaccg gtatcaccag gggagcaagt ttgctgattt
     4321 tcgcagcgac tccacccttg agtttgtcca tctcgtagcc acgggcgcgt tgggcatcca
     4381 tggtgatggt gaggtcccgc gcgaaggttg gcagaaagcg gaacgccaaa tcaatggtga
     4441 acgcgacctt gtctgatgcc ttcaactgtt tgaaggtggt gccgtagata ttagggtcga
     4501 tcgtgtatgg gatgagtatc gccgggagcg ccatggtaag tatgcggatg atctggctca
     4561 tggcgaacca taatttcgaa atggtcacag tcaatcccca ggtggtgcct gggatcaatg
     4621 cttgaaacag aatcggggaa ttcgggtcat tcacggagga aggaccgccg cgcccggaaa
     4681 gtaagccatt caacccaacg atgaaaaaca ccaggatgaa aataaagaac aggggccgcc
     4741 gtatgtccct ccactcgatc cgcgccatca aaaactgggt cagcgaaaaa agaaagagtg
     4801 gaacgagaat gcggtaatcc cagatctctg gaatcgtgag tgccaagatc gtacacaaca
     4861 gcagaatgaa tctggtgcga ggatctaaac gctggatgaa ggtgttgcgc gggcggtact
     4921 tccaggtgac aagcattcgg gctatcggcc tctgcgcgcg gcgactcctg caaaagcgat
     4981 catcagaatc ggcaggagga tggcaaccac aacgagatta ccaatgaagg cgggtgtgaa
     5041 gtaacccacc agcgcagtgc tcaggtcaat cccaccgacg aacatttcag tgagcgaagc
     5101 aaataacatg cctactgcta caccgagcgc gccccagcca acggccttaa taatatcggc
     5161 acggtctttg aaatcggtga tggctgccat gatcaaaccg ggaactagtc ccatcaagcc
     5221 attccctaag ctccaattcc aatagaaacc aaagcctgaa atcagatctc cgagagtgtt
     5281 gccgataaaa cctgcaagga aaccgacgat cggcccatac gcaacaccga agaaaattaa
     5341 tacggcaacc gccggtcgga aggtgatgtt accggctgcg gggagtggaa tgaaatttga
     5401 cacccaggat aacgcgccgt agagtgccgc gccgatggca ccatacacaa cctgccgtgt
     5461 accgaactcc catgttgaat tgttgttcat tgcttctctc cttgttttaa aaagttgaat
     5521 agacgagctg acccaacttt gataaccctg taacagggtt acagttgcac ggaaatgcta
     5581 cctttctttt tgagagcaat agccctcctc gcaacgacat gtatcaagca taggctgcca
     5641 gggcctctgc cgtcaggaac tgtttcgtac gcgggagcag gtcaaggttc atgcgcagga
     5701 gggaagtcgg gcgtacacga tagcgcgtca gcaaatcgaa atccttgagc acctcttccg
     5761 gcggtccatc ggcgacgaca tgcttatcgc cgaacagcaa aacgcgatta gcataggtga
     5821 tggcgagatc aaggtcatgg gtgatgaaaa tcgtcgctgc aaagtttgca gcgaccagcg
     5881 attttgcgcc gttcaccgga cgacacatcg catccatgaa gtgagtgtag tttgcataat
     5941 cctgcccggc cgtgggctca tccatgatca tgattttgga ttgcatcgca agcacggcag
     6001 cgatggttgt acgcttttgc tggccaaatg aaaggcccag cggcgggtat tcctcaaatc
     6061 ccttcagatt cacggtggaa acactttcag ccacagatcg cttaatcgcc accgggttga
     6121 attccaggtt tttgggacca aactctaact cttcgcgcac cgtgggtgca tagagcatat
     6181 gtgtcgggct ttgaaacacg aaacctagcg tacgcgcaat ttgtgcaacg ctcatcgtgc
     6241 gcgtgtcatt tccatccatt aggacgcgcc cacgtgtcgg cttcaacaaa ccaatcgtct
     6301 gtttcaccag tgtggacttc cccgcaccgt ttgggccgag tattgccacc agatcaccgg
     6361 gataaatttg cagattgacg ttctgcagga tcagcggcaa ctcttcatca tagcgaaagt
     6421 ccacatcctc aaagacgatc aacggctcgc ctcgttcaga atttttggcg ggctctgttt
     6481 tttcaatttt ctctcccagc tgtcgaactc gtttcaccac ccattccgca ggaagcttga
     6541 cttcgtgatg atttatctct gtcggaagcg catcaatcgg acccaggtac ttgatctgac
     6601 cagcctccat atagagcagc cgatcaggct tggcaacgat tgcgtcctct acgcgatgct
     6661 ctatcaatac gatcgttttt ccttcatcgg caaggctacg gaaaagctcc aatgcctcgt
     6721 gcgctgaagc aggatccaga gaggcgaggg gttcatccaa caataa
//
LOCUS       DASTJP010000847         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_293663, whole genome shotgun sequence.
ACCESSION   DASTJP010000847 DASTJP010000000
VERSION     DASTJP010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293663"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFQ23_24590"
     CDS             <1..480
                     /locus_tag="VFQ23_24590"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9909850.1"
                     /translation="YDEYNDSARSNLVAIGVHEIAHQWWFGLVGNDQATEPWLDEAMA
                     IYSEALFYKYIYPNSSDWWWNFRVNYFGPTGWVDTTIYEVGTFREYVNATYLNGANFI
                     EALNYRMGDDDFFRFLKDYTSRYGRGRATAYDFFAVARQNTTADISDLINAYFKGSY"
     gene            497..1432
                     /locus_tag="VFQ23_24595"
     CDS             497..1432
                     /locus_tag="VFQ23_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909851.1"
                     /translation="MMKIYSVILGFLLAGCSSNEPAATTIPNILPTSSPIPTETVPPP
                     TPTLVAVVPSPIFTSSPIWTPFPTLSSTAAEETLSLWIEGVFDCLLPCWGGITPGVTT
                     WEETRQVMEQLSGFATVNISEGMNCEFGGCNGIAWSLYPNTLAEGVFYTRLPENKIHL
                     IHINIQNEGNAQKINLLRGIGLQEAFKWYGLPPIFLLTVETDQAQNRLMKLVLVYPGR
                     QSIIQYTKKTELIDGKLTNCGPDHQIEMIILDNKEQLSSLDFIANSVETRDLRLDARY
                     KTVEEATGITSNTFYNAVTSFNDFCISTPADMWVP"
     gene            1507..2364
                     /locus_tag="VFQ23_24600"
     CDS             1507..2364
                     /locus_tag="VFQ23_24600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9909852.1"
                     /translation="MELEHSYIETNGIRLHVVQAGPKSGTPVLLLHGFPENWRCWIRQ
                     LPALAEAGCRVIVPDQRGYNLSDKPDGIKNYQMEVLTRDILGLIDKLEYEKINLVGHD
                     WGAAVAWSLGIQHPERLHRLGILNVPHPAVMKRFLRRDLEQLRRSWYIFFFQLPWLPE
                     AGMRFQGWQALVRSIKDSGKAHTFSDEDIENYKEAWSQPGAISAMLNWYRAAARYPIE
                     PTGELCVRVPTLMMWGMHDNALTHRMARPSMDYCKEGRLLFFPEATHWVQRDEAEEVN
                     RHLLDFVFM"
     gene            complement(2392..>2652)
                     /locus_tag="VFQ23_24605"
     CDS             complement(2392..>2652)
                     /locus_tag="VFQ23_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET9909853.1"
                     /translation="LWRQILASVLDAELVTVNTTEGAAYGSALLAGVGAGAWNDVPSA
                     CKVSVKITGSTLPHPTQVEVYQTAYSLYQELYPALIPSFSQM"
BASE COUNT          669 a          614 c          668 g          701 t
ORIGIN      
        1 tacgatgaat acaatgattc ggcgcgcagc aaccttgtgg cgatcggtgt acacgaaatt
       61 gcgcatcagt ggtggtttgg tctggtcgga aatgaccagg caacggagcc gtggctcgat
      121 gaagccatgg cgatttacag cgaagctttg ttttataagt atatttatcc caactcgagt
      181 gattggtggt ggaatttccg cgttaactat tttggtccaa ccggctgggt ggatacgacc
      241 atttatgaag ttgggacatt ccgggaatat gtcaacgcga cctatctcaa tggtgccaat
      301 ttcatcgagg ccttgaacta tcgaatggga gatgatgact tctttcgttt cctaaaggat
      361 tacacctcac gttatggtcg gggacgcgcg accgcttatg atttcttcgc ggttgcgcgc
      421 cagaacacga cagccgatat ttcagatttg atcaatgcat atttcaaagg gagttattaa
      481 tagaaggaaa tcgttcatga tgaaaattta ttctgttatt ttagggtttc tactggcagg
      541 ttgttcatcc aacgaaccgg cggcaaccac cattccgaat atactgccca cgtccagccc
      601 aattccaact gagaccgtac caccgcccac tccaacacta gtggcagttg taccctctcc
      661 aatatttacc tcctctccga tttggacgcc tttcccgacg ctttcctcca cagcagcgga
      721 ggaaacgctg agcctttgga tcgaaggggt cttcgattgc ctgttgccat gttggggtgg
      781 gataacgcct ggggtaacga cgtgggagga aaccaggcaa gtgatggaac aactttctgg
      841 ttttgcgacg gttaatattt ctgaaggcat gaactgcgaa ttcggaggct gcaatggtat
      901 cgcatggtca ctttatccaa acactctggc agaaggtgtg ttttatacca gactgcctga
      961 aaataaaatt catttgattc atataaatat ccagaacgag ggtaatgctc aaaaaatcaa
     1021 tttgttgaga ggtattgggc tgcaggaagc tttcaaatgg tatgggttac ctcccatctt
     1081 tttgcttact gttgagacag accaggctca aaaccggtta atgaagttgg ttttggtcta
     1141 tcctggacga caatccatta ttcagtacac aaagaagact gaattgatcg atggtaaatt
     1201 gacaaattgt ggacccgatc accaaattga gatgatcatc ctggataaca aagaacagtt
     1261 gagttctctg gatttcattg ctaattccgt ggagaccaga gatttacgac ttgatgctcg
     1321 ctataaaact gtcgaggaag cgaccgggat cacatccaat actttttata atgccgtcac
     1381 ttccttcaat gatttttgta tttccacgcc tgcggacatg tgggttccat aataggctaa
     1441 tccccacttc ataattgtct gttagaatgc tgcctcgctt tgtttggagg cagatatact
     1501 ttccccatgg aattagaaca tagttatatt gaaacaaatg gcatccgctt gcacgttgtg
     1561 caggccgggc cgaaaagcgg gacgccagtc ctgctcttgc atggatttcc agaaaattgg
     1621 cgatgttgga ttcggcaact gccagcatta gcagaggcag gctgccgggt cattgttccg
     1681 gatcaacgcg gctataactt gagcgataaa cccgatggaa tcaaaaatta tcaaatggaa
     1741 gtattaacca gggatattct tggtttaatt gataaattgg agtatgaaaa gatcaacctg
     1801 gtcgggcacg actggggcgc ggccgtggcg tggtcgctgg ggatccaaca tcctgaacgg
     1861 ttacatcggc ttggtattct caacgtgccg cacccggcgg tgatgaagag attcctgcga
     1921 cgagacctcg agcaactgcg ccgctcctgg tatatcttct tctttcaatt gccctggctc
     1981 cctgaagcag ggatgcgttt tcagggatgg caggcgttag tgcgttcaat aaaagatagt
     2041 ggaaaagcgc atacattctc agacgaggac attgagaatt ataaagaagc ctggtcgcag
     2101 cccggcgcca tcagcgctat gcttaactgg tatcgcgcgg cagcacgtta tccaatcgag
     2161 ccgacaggtg agctatgtgt tcgtgttcct acgcttatga tgtgggggat gcatgataat
     2221 gcactaacgc accgaatggc gcgcccgagc atggattatt gcaaggaagg ccggcttctc
     2281 tttttccccg aagcgaccca ttgggttcaa cgcgatgagg ctgaggaagt aaacaggcac
     2341 ctgctcgatt tcgtatttat gtaagccgcc cgtatccatc gtcgttcttc atcacatctg
     2401 tgaaaaactg ggtatcaagg ccgggtacaa ctcctggtag agcgaatatg ccgtctgata
     2461 cacctcgacc tgtgtgggat gtggcaatgt actccccgtt attttgacgg acactttgca
     2521 ggcagacggg acgtcattcc acgcgccggc gccgacgcct gccagcaatg ctgacccata
     2581 tgccgcgcct tctgttgtat taacggtgac caactccgca tccaatactg aagcaaggat
     2641 ttgcctccat ag
//
LOCUS       DASTJP010000848         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1437798, whole genome shotgun sequence.
ACCESSION   DASTJP010000848 DASTJP010000000
VERSION     DASTJP010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437798"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFQ23_24610"
     CDS             complement(<1..510)
                     /locus_tag="VFQ23_24610"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET9909854.1"
                     /translation="MTQTYWEKTNVSPVKFTGTSQNRAVMNWLLIFICVVAFLIVYGA
                     FVRLTRSGLSITEWNPISGTVPPLSAEAWQEEFTKYQGTPEFRQINFNMTLDEYKYIF
                     WIEWIHRFLARLVGLIYAIPVFYFLFTKKIPLKEFGIYFVMGLLFIAQAFAGWIMVAS
                     GLVDRPAVSH"
     gene            complement(714..>2415)
                     /locus_tag="VFQ23_24615"
     CDS             complement(714..>2415)
                     /locus_tag="VFQ23_24615"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9909855.1"
                     /translation="GGTGPIAMAQVKQLEKPKQEADSGLDPIAEARKKALTRLAEILF
                     EYTTDDGAAQQQRRGLQALMRGTGQLSMQSSEQTKVVLHLGQAIDAQSKGNDNQAAEE
                     LEHALEAGFNHPALYFDLGLLKSNGDRLESAIRHLQHAVKHNDFGLAARLLMGQINQK
                     MERPGPASVEYLEALKLADAMIVPAEQSDEIRQMYEPLIEAQAMQTDEASLKRLCENI
                     NGMLMRKNWRNNLHRSREQMPKSAEGDMLMPLAEVILQAQSSQVLESISHVHQLARSG
                     RLRSAMDEAFQALTYAPTYLPLHTLMGDLLVRENHTQEAIAKYSVVAQAYSVRGEAGQ
                     SAKMLRRVIQLAPMDMAARTKLIDQLVARGQVDEAIREYIELADIYYRLAELDMARKT
                     YTIALRFVQQANADRQWNVHILQRMADIDLQRLDWKQAIRVYEQIRTLRPDDEGVRKH
                     LIELSLRMGQPAQAAAELESYLSYLQTNNHGEQGIPFVEDLLTERPDDPVLRRALAQQ
                     YQQAGRISEAVAQLDSLAESLLTSNKKEEAMVVINQILLIGPPNSEQYRQLLMQLQSG
                     "
BASE COUNT          557 a          636 c          616 g          606 t
ORIGIN      
        1 gtggctaacc gctggccggt caacaagccc gcttgcgacc atgatccaac cggcaaatgc
       61 ctgggcgatg aagagcaacc ccatcacgaa gtaaatgcca aattccttaa gagggatttt
      121 ctttgtgaaa agaaagtaga aaacgggaat ggcataaatc aaacccacaa gtctcgccag
      181 gaagcgatga atccattcaa tccaaaaaat atatttatat tcgtccagcg tcatattgaa
      241 attgatctgc cggaattcag gcgtaccctg atacttcgtg aactcttcct gccaggcttc
      301 tgcacttagt gggggaactg ttccactgat gggattccat tcagtaattg ataggcccga
      361 acgcgtaagg cggacaaagg caccgtagac gataagaaaa gccaccacgc agataaagat
      421 aaggagccag ttcatgacgg cgcgattttg cgatgtgccg gtaaatttca cgggcgaaac
      481 attggtcttc tcccagtacg tttgtgtcat tcgttagaaa ctccaaatat aaggtcgtgg
      541 ggattatagc attcccctcg cgcttagcgg agccgtaggc gcagacgaag cgcaagtatc
      601 ttggcaaata cccttcgcct acgggcttcg ttatcactta ttatcactta gcccttcgct
      661 cagcgcggag gcgataacaa aaacccgccg ttcgtgcggc gggcgttgat acgttatccg
      721 gattgcaact gcatgagcaa ttgacgatat tgttcggaat tgggcggtcc gattaacagg
      781 atctggttga tcacgaccat ggcttcttct tttttattac tcgtcaacag cgattcggcc
      841 agcgagtcaa gttgtgcgac cgcttctgaa atgcgtccgg cttgttgata ttgctgtgca
      901 agcgcacggc gtaacaccgg atcatctggc cgctcggtaa gcaaatcctc gacaaacggt
      961 atgccttgtt cgccgtgatt attggtttgt aaatacgaca agtagctttc gagttctgca
     1021 gctgcctgcg ccggttgacc catacgcaag ctaagttcta tgagatgttt ccgtacccct
     1081 tcatcatcgg ggcgcagggt gcggatctgt tcatagacac ggattgcctg tttccaatcc
     1141 agtctttgca ggtcgatatc agccatacgc tgcaaaatgt ggacgttcca ctggcgatct
     1201 gcattggctt gttggacaaa gcgcagagca atcgtgtatg tcttgcgtgc catgtcaagc
     1261 tcggccaggc gatagtaaat atcggcgagt tcgatgtact cacggatggc ttcatccacc
     1321 tgtccacgcg caaccagctg atcaattaat tttgtccggg cagccatatc catcggcgca
     1381 agttggatta cacgccgcag catttttgcg gattggccgg cttcgccacg gacactataa
     1441 gcttgtgcaa caacagagta cttggcaatg gcttcctgag tatgattctc acggaccagc
     1501 aggtcgccca tcaatgtatg taaaggcaga taggttgggg cataggtaag cgcctggaag
     1561 gcctcatcca tggccgagcg gaggcgacct gaccgcgcca attgatgtac atgactgatc
     1621 gattcaagca cctgactgga ttgggcctgc aggatgacct ctgcaagcgg cataagcatg
     1681 tcgccctctg cggacttggg catttgctcg cgggagcgat gcaaattatt tcgccagttc
     1741 ttgcgcatca acattccgtt gatgttttcg caaaggcgtt tgagcgaagc ttcatcggtc
     1801 tgcatggcct gtgcttcgat gaggggttcg tacatctgcc ggatctcatc cgattgttca
     1861 gccgggacga tcatcgcatc tgccagcttc aatgcctcga gatactcaac cgacgcaggt
     1921 ccgggtcgct ccatcttctg attgatctga cccatcagca accgcgccgc gagtccgaaa
     1981 tcattgtgct taactgcatg ttgcaaatga cggatggctg actccagccg atctccattt
     2041 gatttaagta gacctaaatc gaaatacaag gcaggatgat tgaatccagc ttcaagagcg
     2101 tgttccagtt cctcggcagc ctgattgtca tttcctttgc tttgcgcgtc gattgcctga
     2161 cccaggtgca acacaacttt cgtctgctcg gaggactgca tcgaaagttg acctgtgccg
     2221 cgcatcaacg cctgtaaacc gcgtcgttgt tgttgagccg cgccatcgtc agtagtgtat
     2281 tcgaagagaa tctctgccag ccgagttaac gcctttttgc gcgcttccgc gatcgggtcc
     2341 agccctgaat cggcctcctg tttcggcttc tccaattgct tgacctgcgc catcgcaatc
     2401 ggccctgtcc cgccc
//
LOCUS       DASTJP010000849         8701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1138792, whole genome shotgun sequence.
ACCESSION   DASTJP010000849 DASTJP010000000
VERSION     DASTJP010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138792"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFQ23_24620"
     CDS             complement(<1..389)
                     /locus_tag="VFQ23_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909856.1"
                     /translation="MIRVLLNIPSPALRAGLRALLSSDNSLQIINDSLDQDDPGSEAD
                     VVITSASPVLFSKHLPAINTNNGLHDSPSAAILFLSDEQVNLRELRRLSGTWGILPSD
                     ASAEELTAAVHALAQGLIVGTSTLLFES"
     gene            complement(386..1282)
                     /locus_tag="VFQ23_24625"
     CDS             complement(386..1282)
                     /locus_tag="VFQ23_24625"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9909857.1"
                     /translation="MPNPLTDFSNNLTAAVEKGGASTILVDARKRYPASGIAYAEDLV
                     LTADHVVTREENIKIITSDGKSLSATIAGRDPGSDLALLRLAEKALSPAKTANDVKVG
                     QLVLALGRPSSAGVQASWGIVASISGPTRTFRGGMLDEYIQTETTPYPGFSGGPLVNT
                     EGEILGLNTSGLTRGSALTIPVKGAWQVADALAKHGSVKRGYLGVRSQPVEIPETARQ
                     ALKREQNHGLLVLWLEESGPAAKGGLFVGDILVGVNGQTVSDPDDLFSALGSDTVGKS
                     VAVELLRGGRPESVNVTVGERK"
     gene            1415..2704
                     /locus_tag="VFQ23_24630"
     CDS             1415..2704
                     /locus_tag="VFQ23_24630"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9909858.1"
                     /translation="MQASVRLNAKIFPVISVVALLMQIIDPSRVWVILSIGLGGTWLI
                     SRWWARGLARSLHFEREMRFGWAQVGDQLEERFTLRNDFILPATWVTIQDHSTLPDHK
                     ASVATGVDGSSTSQWRILTRCTRRGVFTLGGTSLETGDPLGIYTITFEDRTSSTLAVM
                     PPVLALPKFHINSSGWAGEGKTNPRSLEETIHASHTREMTPNDPMRWVHWKSTAKYNK
                     FYVRQFEGTPAGDWWILLDLHKDSQLGNGWDSTEEHGVILASSLAAQGLDEDHPVGIA
                     INSDEPVWLVPRRNEYQQRSILKSLAVASLADLNLKQYLQRVGSALSSHSSLLIITAH
                     TDGDWASSLLPLIWRGIRPTVFLLDPISFGGKTTTAGLTDALQSLGVPCHTIPRELLD
                     TPKARPGQEGEWEWRVSATGKAILVQAPIHSDWRRLG"
     gene            2629..5067
                     /locus_tag="VFQ23_24635"
     CDS             2629..5067
                     /locus_tag="VFQ23_24635"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HET9909859.1"
                     /translation="MAGFCNREGHPRPGADSLRLAEAGLILRLIRRLLTANVMGVLLV
                     LLSLQLLTSGISSSLRDTDTKNLFPICLVAALIGVGLSRSKFNGIKAVVLVVVLGFIG
                     VWVMGAGLIIPIINLLKTIAPVIPQLNPALRAPVIVDLESIADAWLVILQASAALTAR
                     LQTWIFGLNRNVLVNDALIRNMMWILIVWLFAAATGWFAGRRNAIASLLPSVLLLALV
                     TSYSERNIESFWLMIAMLLLLMGIWNYKNHTQQWEKQKVDYSESISYDTGQAVFFFTV
                     AIGLIAFFTPSISWREIREFLRERNQTSQNEAAEMLGIQQQALPSQNISQPKPSLPRD
                     HLLTGGFAQSQKIVMTISTGELPPIPNPSLIGDVPQYYWRSVTYDVYVGEGWVTSSAP
                     PSKYEANTPLIPGLLDQHRLVHLDVQMFQPEGKLFWSGILFSADIPFTADWRVRPQPS
                     LFADQSTLLQADMFAAASNANSYKAQSYVPDVTEDQLRGSSTEYPESIRDRYLLLPRS
                     VPQRVRALAQEIVAGKTNPYDKAKAIERYLRTYPYDLEVPAPPEEQDVADYFLFDLKR
                     GYCDYYATAMVVLARASGLPARFVSGYSSGSYDAPNAQYVVRELNAHSWAEIYFPEIG
                     WVEFEPTASQPEIDRMLEEAILPPQGSEEATASTLLNRYRTGLVLIWASPLIAILFIA
                     LLYFTIVERWYYMRLSPNTAIERIYRRLYRFGRPLVGNRSSGETAFEFSNRLNRKIDE
                     VRHRSGRSNLFHKIKENVQELTDIYYRSLFSQQHTQKKDAVMAFNIWKQLRIRLIATQ
                     IVNRLNKNKITSTY"
     gene            5166..5657
                     /locus_tag="VFQ23_24640"
     CDS             5166..5657
                     /locus_tag="VFQ23_24640"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET9909860.1"
                     /translation="MVSMDSLVVKTENKFRSLITKQAYTKGQVICEIPTEKLFDKANR
                     FTVQIGRDKHTEVGKLAALNHSCDPNVILDTENLSMVARRDIEKGEELSFFYPSTEWE
                     MDAPFICLCGSSNCIHVVAGARFLPLSTLETHYLNRHIREMMIELLNNTESHLTGLLQ
                     VQK"
     gene            complement(5730..6146)
                     /locus_tag="VFQ23_24645"
     CDS             complement(5730..6146)
                     /locus_tag="VFQ23_24645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909861.1"
                     /translation="MSPIINPDSAGKERTRLTKAIVLAVRQLAKQSQVNEEAKDLAAF
                     ISLALRIISDGIDVSVAAWEKRDYWIKADKFRMEWMWTGQVADKMKVAIFTNDWGTVA
                     MLSAQIAQKFNKIEVSDNHRLGRPWEGAFQKMLTDA"
     gene            6285..6366
                     /locus_tag="VFQ23_24650"
     tRNA            6285..6366
                     /locus_tag="VFQ23_24650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6319..6321,aa:Leu,seq:tag)
     gene            6427..7962
                     /gene="tig"
                     /locus_tag="VFQ23_24655"
     CDS             6427..7962
                     /gene="tig"
                     /locus_tag="VFQ23_24655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET9909862.1"
                     /translation="MKIEKVIEDGHQAKLIVEVEPEKMDTYKRRAARSISQRGKIAGF
                     RPGKAPYDMVVRSYGEQAIVEQAMDLFIDAEYSNILKEAEVEPGASGALESIDSLEPP
                     KLTFRVPLAPEVDLGDYRALRLPYEWTAPEDKDVEAAIEDLRQMYATTENVEREAQVG
                     DYVLVDVKSEITELNRTGFAAVIREEDRDTEWPYAGFARELLGLKAEDTKTVQHKFPA
                     DHDLEALQDKDAVLEVTVKTVRAVTLPELDDEFAKTVGAGETLDALREAVKKDVENRS
                     KADYDDKYFVDLIEKIKEGATLKYNEHTLEHEEEHVLEDLSQRLAGQNMDLDTYFKLR
                     STTREQFIADEVKPVAQKRLERSLILDELVRKEKIEVDNESLDSEFNQTLNALSMQGM
                     DFGKIRGGKKGQQRVAQAVAMESANRVLTRRALDMLKTIATGQYKTAEDAKADMISEG
                     GPVMTEEQAVESEPSTTGAKTEEAKADMVSEGAPVMTEEQAVETEPTSESETETDPTK
                     SSE"
     gene            8029..8637
                     /locus_tag="VFQ23_24660"
     CDS             8029..8637
                     /locus_tag="VFQ23_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9909863.1"
                     /translation="MIPNFKNIVPMVVENTGRGERAYDIYSLLLKERIIFLGTPIDDQ
                     VANVIVAQLLFLNHEDPEREIQMYINSPGGIIYAGLAIYDTMQMISNPISTVAVGVTA
                     SFGTVLLTAGTKGRRYALPNATIHMHQPLGGAQGQATDIEIQAKQILRLKSLLLGIMA
                     KHTGQPLEVIERDSDRDYYLEAKGAVDYGLVDQVLEAPVKVE"
BASE COUNT         2237 a         2162 c         2198 g         2104 t
ORIGIN      
        1 gattcaaaca acagcgtgga tgtgccgacg atcaatccct gtgccagcgc gtggacggcc
       61 gcggtcaatt cttcagcaga tgcatcggac ggaaggattc cccaggtccc ggacaggcgt
      121 ctcagctcgc gcaggttgac ctgctcatcg ctcaggaaca ggatcgcggc ggaaggcgag
      181 tcatgcaatc cgttgtttgt gtttatcgct ggcaaatgtt ttgaaaataa aacaggcgaa
      241 gccgacgtga ttaccacgtc ggcttctgac cctggatcgt cctggtccaa cgaatcattt
      301 atgatttgta atgaattatc cgaggacaac aacgctcgta accccgcgcg tagtgctggt
      361 gatgggatat tcagcagaac tcgaatcact tcctctcacc taccgttacg ttgacactct
      421 cagggcggcc gccgcgtaat agctccacag cgactgattt accaaccgta tcgcttccaa
      481 gggccgagaa caagtcatcc ggatcactca ccgtctgacc gttgacgcca accagaatat
      541 caccaacgaa tagcccgcct ttcgcggcag gtccactttc ttccagccat aaaacaagaa
      601 gtccatgatt ctgttcacgc ttgagggcct gacgcgccgt ctcagggatt tctacgggct
      661 gggaacggac tcccagatag ccgcgcttga ctgaaccatg ctttgccagc gcatccgcaa
      721 cctgccatgc tccctttacg ggaatggtca aagccgaacc gcgcgtgagg cctgaggtat
      781 tcaatcctag gatttcgcct tctgtgttaa ccaatggtcc gccagaaaaa ccagggtaag
      841 gggttgtttc ggtttggatg tattcatcca acataccgcc gcggaaagta cgagtcgggc
      901 cgctgatcga agcgacaatg ccccaggatg cctgtacccc tgcactgctc ggacgaccca
      961 gcgcaagcac cagttgacca actttgacat cgtttgctgt ctttgccggg gataacgctt
     1021 tttcggcgag gcgcagaagg gcaaggtcag agccggggtc gcggccggcg atggtcgcac
     1081 taagtgattt gccgtctgag gtaattatct tgatgttttc ttcccgcgtg acaacatgat
     1141 ctgcggtgag caccaggtct tctgcatacg caattccact tgccggatat cgcttgcgag
     1201 catccaccag gatcgtgctg gcgccgcctt tttccacggc agcggtaaga ttgtttgaaa
     1261 aatcagtaag agggttgggc atattatctc cttataacat agggatttgg cgaaagccaa
     1321 gcatcgtcag tgtactttat ataaaaatca aatacatcag gcggatgggc ggttgttctc
     1381 ctagtcgttc ggggaggtcg tttataatcg aatcatgcaa gccagcgtgc gactcaacgc
     1441 aaaaatcttt ccggtcatca gcgtcgtggc gctacttatg caaatcattg acccatcgcg
     1501 tgtatgggtc attttgtcga tcgggcttgg cggcacctgg ctgatcagtc gctggtgggc
     1561 acgcggactg gcgcgctcgc tgcacttcga gagagaaatg aggtttggat gggcgcaggt
     1621 tggggatcag cttgaagagc gcttcacact gagaaacgat ttcatcctgc ccgccacctg
     1681 ggtaacgatc caggaccatt ccactctgcc tgaccacaaa gcttcagtcg ccacaggtgt
     1741 agatgggtca agcacctccc agtggcggat tctgactcgc tgtacacggc gcggcgtttt
     1801 taccctcggt ggaaccagtc tggaaaccgg tgacccgctg ggaatttata caattacatt
     1861 cgaggatcgc acttcttcta cgctggcagt gatgccgccg gttttagctt tgccaaaatt
     1921 ccatatcaat tcaagtggct gggctggcga aggcaagacg aatcctcgtt cgctggaaga
     1981 gaccatccat gcttcccata cgcgcgaaat gactccgaat gatcccatgc gctgggtgca
     2041 ttggaaaagc acggcaaaat acaacaaatt ttatgttcgg caatttgaag ggacacctgc
     2101 cggtgactgg tggatcttgc ttgatcttca caaggattca cagcttggca atggctggga
     2161 ttccacggag gaacacggcg tcatcctggc ttcatcgctg gcggcacagg gattggatga
     2221 agaccatccg gtggggattg ctattaacag cgatgagcca gtctggctgg tgccgcgtcg
     2281 caacgaatat cagcagcggt ctattttgaa gtcgctggcg gtggcctcgc tggctgatct
     2341 aaacctgaag caatatcttc aaagggtcgg cagtgcttta tctagtcatt cgagcctgct
     2401 cattatcact gcccatacag acggagactg ggcatcgtca ttactacctc tcatctggcg
     2461 cggcattcgg cccactgtat ttctgcttga cccaatctca ttcggcggaa aaacaacgac
     2521 agctggactc acagatgctt tgcaatcact tggtgtgccc tgtcatacta tcccaagaga
     2581 attactcgat acacctaagg cgcggccagg tcaggaaggt gaatgggaat ggcgggtttc
     2641 tgcaaccggg aaggccatcc tcgtccaggc gccgattcac tcagactggc ggaggctggg
     2701 ttgatccttc gcctgatcag aaggttattg actgcaaatg taatgggggt attgcttgtt
     2761 cttttatctc ttcaattact gacttctgga atatcctctt cgctgcgaga tacagacacg
     2821 aaaaatttat ttccaatttg ccttgtcgcc gcgctgatcg gcgtggggtt aagtagaagt
     2881 aaatttaacg gaatcaaggc tgtggtgctg gttgtcgtgc tgggatttat tggcgtatgg
     2941 gtgatgggcg ccggattgat tattcccatt atcaatctcc tgaaaacgat tgctccggtg
     3001 atcccgcaac taaatcctgc actgcgcgca ccagttattg tagacctaga atcaattgca
     3061 gatgcatggc ttgttatcct gcaggcatct gccgcgctca cagcgcgttt gcagacctgg
     3121 atattcggtc tgaacagaaa tgtcctggta aatgatgcgc tcatccgaaa catgatgtgg
     3181 atattgatcg tgtggctatt cgcggcggcg acgggctggt ttgccgggcg gcgaaacgca
     3241 attgcctcgt tattgccatc cgttctgctg ctggcgctgg tcacatcata cagtgaacgc
     3301 aacatcgaaa gcttctggct gatgatagcg atgcttctcc tgctcatggg catatggaat
     3361 tacaaaaatc acacccagca gtgggaaaaa caaaaagttg attactctga gagtatcagc
     3421 tacgatactg gtcaggccgt tttcttcttc accgtcgcaa tcggactaat cgcttttttc
     3481 acaccgtcta tttcctggag agagattcgt gagtttctgc gagagaggaa tcaaacatcg
     3541 cagaatgaag cagccgaaat gcttggcatt cagcaacaag cattaccctc tcaaaacatt
     3601 tctcagccaa aaccctcact tccacgggat catctattga ccggcggatt tgctcagtca
     3661 caaaaaattg tgatgacgat cagcacaggc gagttacctc cgattcccaa cccttccctg
     3721 atcggggacg tacctcaata ttattggcga agtgttacgt atgatgtgta tgttggcgag
     3781 ggctgggtca cgagctctgc ccctccaagc aagtatgagg cgaacacccc actcatcccc
     3841 ggtttgctgg atcagcatcg tctcgttcat ttggatgtgc aaatgtttca gcctgaagga
     3901 aaattatttt ggagtggaat cttatttagc gcagatatcc cctttacagc ggactggcgc
     3961 gttcggcccc agcccagcct cttcgctgat caatctacct tgcttcaagc tgacatgttt
     4021 gcagccgcca gcaatgcaaa ctcatataaa gctcaatcct atgttccgga tgtgaccgaa
     4081 gatcaacttc gtgggtcctc tacagaatat ccggagagta ttcgcgaccg atatctactt
     4141 ctgccgcgct cagtaccaca gcgtgttcga gccttggcgc aggagattgt ggctggtaag
     4201 accaacccgt acgataaagc caaagcgatt gaaagatatt tacgcaccta cccatatgat
     4261 ctcgaggtcc ctgcaccgcc agaggagcag gacgtggctg attacttcct gtttgaccta
     4321 aaaaggggat attgtgacta ctacgctact gccatggttg ttctagcacg tgcgagtggg
     4381 cttcctgcgc gcttcgtatc cgggtattct tccggctcct atgatgctcc caatgcgcaa
     4441 tatgtcgttc gcgaactcaa tgcccattcg tgggcagaga tctacttccc tgaaatcggc
     4501 tgggtggaat ttgagccaac agcttcccag cctgagatag atcgaatgtt ggaggaagcc
     4561 atacttccgc cgcagggaag cgaagaggca acagcgtcta ccttactcaa ccgctatcga
     4621 accggattag ttcttatttg ggcatcacca ttgatcgcta tcttatttat cgctctttta
     4681 tatttcacga tagtggaacg atggtattac atgaggcttt ctccgaacac tgcgatcgaa
     4741 agaatctatc gaagactcta tcgctttggg cgtccgctgg taggcaatcg ctccagcgga
     4801 gaaaccgcat tcgaattttc aaacaggctg aatcgcaaga ttgatgaagt aaggcatcga
     4861 tcaggacgtt ccaacctgtt tcacaaaata aaagagaatg tgcaagaact gaccgacatc
     4921 tattacagat ccttgttcag ccaacaacat acacaaaaga aagatgccgt gatggcattc
     4981 aacatttgga agcaattacg catacgattg atcgcaactc agattgtaaa tcgactcaat
     5041 aaaaataaaa ttacatcgac gtattgaatt tcccatatct taaaatttta taaatatatg
     5101 actccagtta ttgattttga ttcaataatg ttgtataact ttaagttgaa agaaaggaat
     5161 tgatcatggt ttcaatggat tcacttgtag taaaaacaga aaataaattt agaagtctca
     5221 tcacaaaaca ggcttataca aaaggccagg tgatttgtga aatacccaca gaaaaactct
     5281 ttgataaagc aaatcgcttc acagtccaga tcggacggga taagcacacg gaagttggga
     5341 agctggcggc actcaatcat tcgtgcgatc caaatgtaat tctcgacacg gaaaatttgt
     5401 cgatggtcgc gcgacgagat atagaaaagg gagaggagct ttcttttttc tatccctcaa
     5461 cagaatggga aatggatgcg ccgttcatct gcctgtgtgg ctcatcgaat tgtattcatg
     5521 tggtcgcagg cgcgcgcttc ctgccgcttt ccacgctgga aactcattat ctcaacaggc
     5581 acatccgtga gatgatgatc gagctgctga acaataccga gtcgcacctg acgggcctgt
     5641 tgcaggtcca aaaataatca agtggtgaga taaaacaaac aggtcttgat acatgtcaag
     5701 acctgtttgt tcttaaagtg gctggttcat tacgcgtctg tcagcatctt ttgaaaggcc
     5761 ccttcccagg gtcttcctaa cctgtgatta tctgaaacct caatcttatt aaatttttgt
     5821 gcgatctgcg cagaaagcat agctaccgtt ccccaatcat tcgtaaatat ggccactttc
     5881 atcttgtcgg caacctggcc ggtccacatc cattccatgc ggaacttgtc ggctttgatc
     5941 caatagtcgc gtttttccca cgccgcgacg gaaacatcga tgccatcaga aatgattcgc
     6001 aatgcgaggg agataaatgc agccaggtct tttgcttcct cgttaacctg gctttgttta
     6061 gccaactgtc gtaccgccaa tacaatggct ttcgtgagtc gggttcgttc ttttcctgca
     6121 gaatcgggat tgataatcgg gctcaaatct atactccaaa gttgggttca agtgcgggat
     6181 tataccaatg aaaatcgcaa aggatttaca aatttctgac agttccctct gcataccttg
     6241 cggtataatt tcgccgctta ccaaatacac gaaatactac tcgcggggac gtgtcggaac
     6301 tggcagacga gcatgactta ggatcatgtg ccgcaaggcg tgagggttcg attccctccg
     6361 tccccatact caccctcacc cctaccctct cccaaaggga gagggagtac cgtatggaga
     6421 agaaaattga aaatcgaaaa agttattgaa gatgggcatc aggcaaagtt gatcgttgag
     6481 gtcgagcctg aaaagatgga tacatacaaa cgccgcgcgg cacgcagcat atctcaacgc
     6541 ggcaagatcg ccggttttcg cccgggcaag gcaccctatg atatggtggt gcgcagctat
     6601 ggcgaacagg ccattgttga acaagccatg gatttgttca ttgatgccga atattccaat
     6661 atcctaaaag aagcggaagt agaacccggc gcgtccggcg cgctggaatc aatcgattcg
     6721 ctggaaccgc ctaagttgac cttccgcgtg ccgcttgctc cggaggtgga tcttggcgat
     6781 tatcgcgcac tgcgtttgcc atacgagtgg acggctcccg aggataaaga tgtggaagcc
     6841 gccatcgaag acctgcgtca aatgtacgcc actacagaaa acgtcgaacg tgaagcgcag
     6901 gttggggatt acgttctcgt tgacgtaaag tctgaaatca ctgaactaaa tcgtaccggc
     6961 ttcgcggccg tcatccgtga agaagatcgc gacacagaat ggccgtatgc tggttttgct
     7021 cgtgagctcc ttgggctaaa agcggaagac acgaagaccg tccagcataa attcccggct
     7081 gaccatgact tggaagctct gcaggacaaa gatgcggtgt tggaagtgac ggtcaagacc
     7141 gtccgtgcgg tgacgctgcc cgaattggat gatgaatttg ccaagacggt gggagctggc
     7201 gaaacgctgg atgctttgcg cgaggcagtt aagaaagatg tcgagaaccg ctccaaagcc
     7261 gactatgacg ataaatattt cgtggatctg atcgaaaaga tcaaagaagg cgcaacgctc
     7321 aaatacaatg aacacaccct cgaacatgaa gaagagcatg tccttgaaga cctgagccaa
     7381 cggcttgctg gccaaaatat ggacttggat acctacttca agctgcgaag caccacacgc
     7441 gaacaattca ttgcagatga agttaaaccg gttgcacaga aacgcctgga gcgctctctc
     7501 atcctcgatg aacttgtacg caaggaaaag atcgaggtgg ataatgagtc attggatagt
     7561 gaattcaacc agacgctaaa tgcactttcg atgcagggca tggatttcgg aaaaatccgc
     7621 ggcggtaaaa aaggtcaaca acgcgtggca caggcggtgg cgatggaatc agccaaccgc
     7681 gtcctcactc gccgcgcctt ggatatgttg aagacgattg caacaggtca atataagact
     7741 gcggaagatg ccaaagccga tatgatctct gaaggtgggc ccgttatgac cgaggaacag
     7801 gctgtcgaat cggaaccaag cacgacggga gccaaaacgg aagaggccaa ggccgatatg
     7861 gtttctgaag gtgcacccgt catgacagaa gagcaggctg tggaaacgga accaacgtcc
     7921 gagtcggaaa ctgaaacaga tccgacaaaa tcatccgaat aaaaacaaaa ttttaacgta
     7981 attccaatac gcagcaggta tgatgtccca atacaaagga gacaaaatat gatccctaat
     8041 ttcaaaaata ttgttccaat ggtggttgaa aataccggcc gtggtgaacg tgcttatgac
     8101 atttattcgc ttctgctcaa ggaacgcatc attttcctgg gtacacccat cgatgaccag
     8161 gttgcaaacg tgatcgtggc acaattgctc ttcctgaacc atgaagatcc cgagcgagaa
     8221 atccagatgt atatcaattc ccctggcgga attatttacg cagggctcgc catctacgac
     8281 acgatgcaga tgatctcgaa cccgatcagc accgtagcgg tcggtgtaac cgcatccttc
     8341 ggaaccgtgt tgttgaccgc cgggacgaag ggccgccgct atgctctgcc caacgcgacc
     8401 atccacatgc accagcctct tggtggtgcc cagggacagg caactgacat cgagattcaa
     8461 gccaagcaaa tcctgcgcct gaagtccttg ctgcttggca ttatggcgaa gcacacaggt
     8521 cagccgctgg aagtgatcga acgcgatagc gatcgcgatt actatctgga agccaaaggc
     8581 gccgtggatt atggtttggt agatcaggtt ttagaagcgc ctgtaaaagt tgagtagttc
     8641 gctaattaga tagtttagta gctgaagccc gttgacattt tgtcagcggg cttcagcttt
     8701 t
//
LOCUS       DASTJP010000850         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_199537, whole genome shotgun sequence.
ACCESSION   DASTJP010000850 DASTJP010000000
VERSION     DASTJP010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199537"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFQ23_24665"
     CDS             <1..389
                     /locus_tag="VFQ23_24665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909864.1"
                     /translation="ETILLVLAPEMASLRTTVSAMEIYDRLGIPLEKVKIALNNNSSN
                     PAIKQAQLEKVLKRPIDFVLPFEAGEVNRALNFGQPFILSNPDLPICLVLEKMAYELS
                     AETQKALPPAVPSPTWKRVTSQLSKK"
     gene            395..>2159
                     /locus_tag="VFQ23_24670"
     CDS             395..>2159
                     /locus_tag="VFQ23_24670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909865.1"
                     /translation="MRTSKRHSFHRILFALGIAVPGLLGMLAVQAKAVTHETLKVEPA
                     LQAQDTSTVTSTPTPPLTATLPFQTSTVVPLATLPPLGNLLPSGLYAFIEAPLGTVPQ
                     PYVILSAFSSLPTQTSVTIQGFINTQEFICTDFPCAIDLQTSSRLVFTAYASSGEVSE
                     TVIASVSVTRTDAGYVVVIGSVSQYRSFTNSCGIAWGVFDEQNVTWDDFVQFPYEMHT
                     KKTLHNLATELLINGIVDASDCPAGGLSLGLSWPTACGLERASGAMIEWQNQFDDHIW
                     LASSQHGIPPKILKTLIEVETQFWPGNTRVFLDEYGLGQVNQLGVDVLLRRDPTLYQR
                     VCPSILSDCLRPYLSLEPQQQAMIRGAVVKLMDVTCADCEYGFDLNKARESIPLVAML
                     FRANCQQVDYILSIADVPDPDADDATATAAAATIAAGGDTDTTSYEDLWRFTILSYHS
                     GLNCFQEALLATRKAHRPFTWENVEERLRCRGGADYVNAFMNNLFAFDFYRLDYTDTN
                     VVLAAPTIIPTRTPISTPTVFVSNATVRVQVYMDRNGNQTPEADEWIDAMTVLLETST
                     NEQITRRTENGIAIFDMSGYTP"
BASE COUNT          507 a          548 c          533 g          571 t
ORIGIN      
        1 ccgagacaat tctgctggtg ctggcgccgg aaatggcttc gcttcgcaca acggtgagtg
       61 ccatggaaat atacgacagg ctgggtattc ccctcgaaaa agtgaagatc gctttaaaca
      121 ataattcctc caacccggcg atcaaacagg cgcagttgga aaaggtgctc aagcgcccaa
      181 tcgattttgt cctgcctttt gaagcaggtg aagtgaatcg tgcgctcaat ttcggtcaac
      241 cctttatttt gagtaatccc gatctaccga tttgtcttgt cctggagaag atggcatatg
      301 agttgagtgc tgagactcaa aaggctttgc cgcctgccgt gccgtcccca acctggaaac
      361 gggtaaccag tcaactgtct aaaaagtaga agcaatgcgg accagtaaac gccattcctt
      421 tcatcgcatt ctgttcgccc tcggaatagc ggttccaggt ctccttggaa tgttggcggt
      481 tcaggccaaa gccgtaacac atgaaacgct gaaagtggaa cccgccttgc aagctcagga
      541 tacatctacg gtcacgagca cgcctactcc acctctcacg gctacgcttc catttcagac
      601 ttcaacagtg gtaccgcttg ctactcttcc acccctggga aatttacttc catctggtct
      661 atacgccttc attgaggctc cccttggcac cgtaccgcag ccttatgtca ttttgagcgc
      721 cttttcctct ctcccgacgc agacttccgt tacaattcag ggctttatca atacccaaga
      781 gtttatttgc accgattttc cctgcgcgat tgatttgcag acaagctcca gacttgtgtt
      841 taccgcgtat gcgagttcag gtgaagtgag cgagactgtg atcgcctctg tctctgtcac
      901 gcgtaccgat gctggatatg tggtggtgat tggctcggta agccaatacc gttccttcac
      961 caattcctgt ggcatcgcat ggggtgtttt tgacgaacaa aatgtcacgt gggacgattt
     1021 tgtgcaattc ccttatgaaa tgcataccaa gaagactctc cataacctgg ctacagagtt
     1081 gcttatcaat ggaattgtgg atgcgagtga ttgtccggct ggcggtctga gtctgggctt
     1141 aagttggcca accgcctgcg gtctggaacg ggcttccggt gccatgatcg aatggcaaaa
     1201 ccagtttgat gaccatatct ggcttgccag cagtcaacat ggcattcctc ccaagatatt
     1261 gaaaacactg attgaggtcg agacccaatt ctggcccgga aatacccgcg ttttcctgga
     1321 tgaatatggc ctgggtcagg tgaatcagtt aggtgtggat gttctgctcc gccgtgatcc
     1381 cactttgtat caaagagttt gtccaagtat cctctctgat tgtttgagac cctatttaag
     1441 cctggagcct cagcaacagg caatgattcg tggggccgtg gttaaactta tggatgtcac
     1501 ctgcgcggac tgtgaatacg ggtttgatct aaataaagcc agggaaagta ttcctctggt
     1561 tgcgatgtta ttcagggcaa attgccagca ggtggattac atcttaagta ttgccgatgt
     1621 tcctgatcca gatgctgatg acgccactgc gaccgccgca gctgccacga ttgctgccgg
     1681 tggagatacc gatacgacta gctatgaaga tctctggcga tttaccattc tttcttacca
     1741 tagtggctta aattgttttc aggaagcctt gctggccacg cgaaaggctc atcgaccgtt
     1801 cacctgggag aatgtcgagg aaagactcag gtgcagaggg ggagctgatt atgtaaatgc
     1861 atttatgaat aatttgttcg cctttgactt ttaccgcctg gattatacag acacgaacgt
     1921 tgtccttgca gcgccgacta tcatacccac gcgtacacca atttcgaccc ccacagtttt
     1981 cgtctcgaat gccacggtcc gggttcaggt ctacatggat cgcaatggaa atcaaacgcc
     2041 tgaggccgat gaatggattg atgcgatgac cgtgcttctg gaaacctcca caaatgagca
     2101 gatcacccgg cgaaccgaga atggtattgc cattttcgat atgtccggtt ataccccgg
//
LOCUS       DASTJP010000851         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1455468, whole genome shotgun sequence.
ACCESSION   DASTJP010000851 DASTJP010000000
VERSION     DASTJP010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455468"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VFQ23_24675"
     CDS             <1..1184
                     /locus_tag="VFQ23_24675"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9909866.1"
                     /translation="DAAYQKAFQRAALSSEGWVAWGDVNYLNENPQRAAEIWEQAFEQ
                     KNPSEQLYSRLGQTYLETGEYSKATDSLQRYVSVYSEDAAAHYQLGLLLTLTDQPTAL
                     SELITASQLDPELDPAVQTLRTALNSASLSELPSERFVVIGRGLGLVEEWQLAQVAFE
                     LSIQADEKNAEAWAWLEEANQHISSKQNEGTPAEANSIEGLDKALSLNPNSSVIRGLR
                     GLYYQRSGNFREALQEFEQAARLEPDNPAWHVSLGETHAKLGDLIRALEAYQHATTLA
                     PEDVNYLRLLAIFCGQNGVHIRDVGIPAAQRVLITLKDDSGAEDLLGWLHLLDARYDD
                     AERHLTRAIALDSQNAAAHLHLGMLYLQQGDRLSAYDHLVLARDLGEAEADQLLKQYF
                     P"
     gene            1258..1896
                     /locus_tag="VFQ23_24680"
     CDS             1258..1896
                     /locus_tag="VFQ23_24680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909867.1"
                     /translation="MRGHLLYLSLLLLFLTGCTAAPIPTVLPANSGDILFQDEFDNHS
                     TGWDRVSNSNGIMDYDQGSYRILVQQPTMNFWSTPEKNFGNVRVEADVIRLNGPDENR
                     VGLICRYQAGDYYFFIISNDGFYAIGKFIGGNTILLGQTEMLSSDFILTNSINHLRAD
                     CIDNTLTFYVNFNQIASVQDADFPSGDVGLLAGSFSESGVDVSFDHFVVMQP"
     gene            1960..3240
                     /gene="mtaB"
                     /locus_tag="VFQ23_24685"
     CDS             1960..3240
                     /gene="mtaB"
                     /locus_tag="VFQ23_24685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HET9909868.1"
                     /translation="MNNMKIYLDTIGCRLNQAEIENMARQFRVAGHEIVPSANMADMA
                     VVNTCAVTNDAASVSRGKIRQIARAGVGEIVATGCWATLQPQQVYDLPNVFRVIPNEH
                     KDHLVSDLLHIPQHTFDVEPISRQPLPGMHRRTRAFLKVQDGCDNHCTFCITTVARGE
                     GRSRPVADVINDIKLALDGGTKEIVLTGVHLGSWGYDFHLQLKDLIKTILSETGVPRL
                     RLSSLEPWDLDEQFFQLWEDSRLMPHLHLPLQSGCEATLKRMARKTTPDSFRALVSAA
                     REAIPNVAITTDIIAGFPGETEEEFAASLAFVNEMNFSGGHVFTYSPRPGTGAARMKG
                     QVKPEVRKRRNHILHDALEASARSYRESFVGTQVAVLWESTSEMGERGWQMEGLSENY
                     LRVRAPAAFPRWNEIDNVRLCTSTENGLEGELIL"
     gene            3315..>3829
                     /locus_tag="VFQ23_24690"
     CDS             3315..>3829
                     /locus_tag="VFQ23_24690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET9909869.1"
                     /translation="MSWENTHKTRTAFILLILIPIFLLVSLSSSKIYDAHKQDFAAYW
                     QAGHMILSGMDIYDSSEWLAERQRLGTALHSEPTFHYPLPFAILFSPLALLPVQTAYT
                     LWLFLGQIAILSSILILLKFHGARTGYFELFTIAGIFLFRPSFSVIFNGQILSLLLLL
                     VSVSILLFHDQR"
BASE COUNT          886 a          937 c          984 g         1022 t
ORIGIN      
        1 cagatgcagc ttatcagaag gcctttcagc gtgcggctct atcgtctgaa ggttgggtgg
       61 catggggaga tgtcaattat ctaaacgaga atccgcagcg cgccgccgag atttgggaac
      121 aggcttttga gcagaaaaat ccgtcggagc aattgtattc acggctcggt caaacctatc
      181 tggaaacagg tgagtattca aaggctaccg attctttgca aagatatgtg tcagtttatt
      241 ctgaggatgc cgcggcgcat tatcaattgg ggcttctgct gacattaact gaccagccca
      301 cggctctgtc ggaattgatc accgcctccc aactggaccc tgaacttgac cccgctgttc
      361 agactcttcg cactgccttg aattcggcct cattaagtga gttgccatca gaacgatttg
      421 tggtcatcgg gcgcggatta gggctcgtcg aagaatggca gctcgcgcaa gtcgccttcg
      481 agttgtccat tcaggcggac gagaaaaatg cggaggcctg ggcatggttg gaagaggcga
      541 atcaacatat aagctccaaa caaaatgaag gaacgccggc cgaagcgaat agtatcgagg
      601 gtctggataa ggcattaagc ttaaacccaa actcgtctgt catacgcggg ttacgggggt
      661 tgtactatca gcgttccggc aactttcgcg aggccttgca agagtttgaa caagccgctc
      721 gcctcgagcc agataacccg gcctggcacg tttcattggg tgagacccat gccaagcttg
      781 gcgatttaat ccgtgcgctg gaggcttatc aacacgcaac aacgcttgca cccgaagatg
      841 tcaattattt gcgtctgctt gccatcttct gcggacagaa tggagtgcac atcagagatg
      901 tgggcattcc agctgcgcag cgcgtcttga tcacgttgaa ggatgattct ggggcagaag
      961 atttgttggg ttggctacat ctgttggatg ctcgctatga cgatgcggag cgacatctca
     1021 cgcgtgccat tgcactggat tcacaaaatg cagctgctca tttgcacctt ggaatgttgt
     1081 atttacaaca aggggatcgc ctgagtgcgt atgatcattt ggtgcttgcg cgcgaccttg
     1141 gggaagctga ggctgaccaa ttgttgaaac agtactttcc gtaaatattg catgtcgttg
     1201 cttcggcaac acaacactgc ctcgcaatga cagtcccaac tctgataaaa tctcaacatg
     1261 cgaggccatc tcctttatct ttcattgctc ctcctgttcc tcactggctg tacggcggca
     1321 cctataccga ctgtgcttcc tgcaaattcc ggagatatcc tgtttcaaga cgaatttgat
     1381 aaccattcca cgggctggga tcgggtctcg aacagcaatg ggattatgga ttacgaccag
     1441 ggcagctatc gaatcctggt gcagcagcca accatgaatt tctggtctac ccctgaaaaa
     1501 aactttggta atgtacgcgt cgaagcagat gtaatccggc tgaatggacc ggacgaaaac
     1561 cgggtggggc tcatttgtcg ttatcaggcc ggagattact atttctttat tattagtaat
     1621 gacgggtttt atgccattgg caaatttatt ggaggaaata cgatcctgct tgggcagact
     1681 gaaatgttat cgtccgattt cattctcacg aattccatta atcacctgcg ggcagactgt
     1741 atcgataata cgctcacatt ctatgtgaat ttcaatcaga tcgcgtctgt acaggatgct
     1801 gattttccct caggagacgt aggtcttttg gcgggttcat tttcagaatc gggtgtggac
     1861 gtttcttttg atcattttgt ggtgatgcaa ccttaattgc ataaatattg tagggacggg
     1921 cagctcgcga agcaccctga tgagggtgcc cttccctaca tgaataatat gaaaatctat
     1981 cttgatacca taggctgtcg cctcaaccag gccgaaatcg aaaatatggc gcgtcaattc
     2041 cgcgtagccg ggcacgaaat tgtcccgtca gcgaatatgg cggatatggc cgtggtcaat
     2101 acctgcgcgg tgacgaatga tgcagcctca gtttcacgcg gcaagatccg tcagattgcg
     2161 cgcgccggtg ttggtgaaat tgtcgcaacg ggctgctggg caacgttgca gccgcagcag
     2221 gtatatgatt taccgaatgt ttttcgggtg atccccaatg aacataaaga ccatcttgtc
     2281 tctgatctgc tacatattcc tcaacacacg ttcgatgtgg aaccgatttc ccggcagcct
     2341 ttgcctggta tgcatcgtcg tacgcgtgcc tttctcaaag tgcaggacgg ctgcgataat
     2401 cactgcacgt tttgcattac aacagttgcg cgtggcgaag gacgaagtcg tcccgttgct
     2461 gatgtcatta acgacattaa attggcgttg gacggcggca cgaaagagat tgtcctaacg
     2521 ggtgtgcatc tcggctcgtg gggctatgat tttcacctgc agttgaaaga tctgatcaaa
     2581 acaattttgt ctgaaacagg ggtaccacgg ctgcgcctct cctccctcga accctgggat
     2641 ctggatgaac agttcttcca gctttgggag gactcacgcc tcatgcctca ccttcatttg
     2701 ccgctgcaat ccggctgtga agccacactg aaacggatgg cacgcaagac aactcctgat
     2761 tctttccgtg cgcttgtctc cgccgcgcgc gaggcgatcc ccaatgtggc tattacaaca
     2821 gacattattg cgggcttccc gggagaaaca gaagaggaat ttgccgcgtc acttgccttt
     2881 gttaatgaaa tgaatttttc aggtggtcat gtctttacct attcgccgcg tccgggcaca
     2941 ggggcggcac gcatgaaggg gcaggtgaag cccgaggtcc gaaagagacg gaatcacatc
     3001 ctgcatgacg cgttggaagc ctcggccagg tcctatcgtg aaagtttcgt cggtacgcag
     3061 gtggccgtgt tgtgggagtc cacgtcagag atgggggagc gcggctggca aatggagggc
     3121 ttgagtgaaa attatttgcg tgtacgtgca ccggcagcct ttccgcgttg gaatgagata
     3181 gacaacgtgc ggctgtgtac atcgacagaa aatggactgg aaggtgaact cattttgtag
     3241 ccttctttca ttgccttgtc gctactcctg agtcgcatcc ttacttacgt gaattcactc
     3301 tctacttcaa caatatgagt tgggaaaaca cccataaaac acgcactgcg tttatcctcc
     3361 ttattttgat tcccattttt ttactggtga gcctctcctc ctcaaaaatc tatgacgctc
     3421 acaaacaaga ctttgcagcc tactggcaag cgggtcatat gatcttaagc ggaatggaca
     3481 tttacgacag ctcggaatgg cttgcggaac gccagcgact gggtactgca cttcactccg
     3541 agccgacatt tcactacccg cttccgtttg caattttgtt ttcacctctg gcactgcttc
     3601 cggttcaaac tgcatacacg ctttggctat ttttggggca gatcgccatt ttgagttcaa
     3661 tccttatcct cctgaagttc cacggtgcac gaaccggtta ttttgagttg tttactattg
     3721 ctggtatatt tttattcagg ccatccttta gtgtgatttt taacgggcaa attctctctc
     3781 tgctattgct gctggtgtcc gtttcgattc ttctgttcca tgatcagcg
//
LOCUS       DASTJP010000852        19189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1317500, whole genome shotgun sequence.
ACCESSION   DASTJP010000852 DASTJP010000000
VERSION     DASTJP010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19189
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317500"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFQ23_24695"
     CDS             <1..654
                     /locus_tag="VFQ23_24695"
                     /inference="COORDINATES: protein motif:HMM:NF020671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HET9909870.1"
                     /translation="ELLITNSFIPLEEGTPSITSEVLVLAAPYWNDLRNKLLKVVDSY
                     HQTYPLRRGIPREELKSRLKLPSRVFNGVMKKLSLDQSLTEYSAYLAKPEHEIRFDNR
                     QESRVGALMQKFEQNPFSPPSIKECQSEVGEEVLNALVEIKELILLSPDVIFRKQDYE
                     FMKSKVQELLVQREKITLAEVRDLFNTSRKYAQAFLEHLDTVGITRRDGDFRRVGKQ"
     gene            651..3425
                     /locus_tag="VFQ23_24700"
     CDS             651..3425
                     /locus_tag="VFQ23_24700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9909871.1"
                     /translation="MNTLKAWLSPPVFDNAEKTHQAYLLHFILLALIAIPIPFVTYLL
                     IRAPEESDRALILIAVSEIIHIILFIILRRGHVQIASFLQVITIWQFLAIASITSSSI
                     YGVAYTLGNAMVITIAGILLGGRGALVLTLLIILEGAILVYAEVQGWDPPDILDDALP
                     TWIVSVVLFAVVASLQNLAAREVRAALNRAQTSEERYRLISEVTSDYTFSTEFNAKGN
                     ISGFWAGGAFEKITGYTIKEYSNLESWRSRLHPDDFEKDAQNTLDLKQNRSFVSDLRL
                     YRKDGDLRWIRIYANPVWNQKEDRLARVVGAVQDITEQRRAEELEQRRRLMMEKVIQL
                     GKHVTEVSDLRTTLERIWHGVHDELDFDRLAIFLHNPKKNFMQGTLGTNNDGQIVEEW
                     EESFPITVGDMFHRVIQKPDGFHFTHNYDIEESVPEGDEMYGVKDFAAVAGWASDKPV
                     AIIVADNLPSGRSITDEKLEALRLFGGYAGLAIENARLHTALQNELRLQKEAEEREVH
                     RRARLEQVILLGQQVTKVSDLRSTLEKIWNGIHFELGFDRLAIFLYDQEANTVHGTFG
                     TDNDGRLVEEWEYVQTLEQQKPSSFLRALEQPNGLFFTRSFGIDFEIPEGHKMHNVQD
                     FVAVSAWAGEKPVAIITVDNLPSGRSITHAHLESLRLFCGYAGLAIENARLNEALQDE
                     LEHRQTLIRELEAKNAELERFTYTVSHDLKSPLVTITGFLGYLERDALDGNVQKIRSD
                     IERIVRAAEKMQALLNDLLELSRVGRLINSPQNVPFGDIVNEALENVRGRLESNKINV
                     KVHDDLPTVLGDKVRLVEVLQNLIDNAAKFTHTSPNPSIEIGTSGLDQEANHIYFVRD
                     NGIGIQPEFQERIFGLFNKLDQTVEGTGIGLTLVKRIIEVHGGKIWVESELGKGATFY
                     FTLPGSKNEE"
     gene            3428..4099
                     /locus_tag="VFQ23_24705"
     CDS             3428..4099
                     /locus_tag="VFQ23_24705"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET9909872.1"
                     /translation="MCGRFTLTIDPAEAQENFSNFTFPEKFAPRFNIAPSQPILAIPN
                     DEKFTADFFMWGLIPMWAKDPSIGSRLINARAETLAEKPAFRGSLKYKRCLILADGFY
                     EWKSSDGKKSKTPFFIHMKDRKPFAFAGLWDSWNSPEGSQLKTCTIITTEPNELMSLL
                     HNRMPVILHPRDYDKWLNPSPQTPDQLTPLLKPFPAEMMDAYPVTPLVNKPANDTPEL
                     VVPAR"
     gene            4120..5382
                     /locus_tag="VFQ23_24710"
     CDS             4120..5382
                     /locus_tag="VFQ23_24710"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9909873.1"
                     /translation="MIHKTQIFLNQRLPALASRDFILFLVGQLISVVGTWMQATALPY
                     LAYRISGRPLDLGLIGFSTTLPTLLFALPAGVFVEHWDKRKAVILLQALMSIQAFGLA
                     FLTYTGQIQIWHIVLLAFLYGSAMAVEVTARQAMLIELAGRDALPSAIALQTTAFNLG
                     RILGPLCAAWLISVTGSEGSVFLVNGISFIFVIGGLFLARTGFKTIEDTNQDRNLGNE
                     FREGIGYIRSEVVVMSVIIMSALVGFFGMPLIQQIPAFARDVLGTIQDTEAIVATRTS
                     NLYAAQGAGALVAAFIAASMHSSDKNRMLLLGQVTFILPIIALGFTSNTAVALILLTF
                     IGWGTVTQLVTMNTIIQTRVPNGLRGRVFSIYFWALQGVAPFGSIFIGWIAQTWNVPL
                     ASIISGAICLLGVVAVRLTFKNRQTSSA"
     gene            5464..6621
                     /locus_tag="VFQ23_24715"
     CDS             5464..6621
                     /locus_tag="VFQ23_24715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9909874.1"
                     /translation="MDNKSPINKNVDSPGFFSRLMQTLVGLGLGESMLRLGTTVLSVI
                     LLGVVIWLVRLFYAQTPARNVANALEAEPTAAVAEIQDISSASVDDFGGIPRLAQVHT
                     IIPSRPRQEIVTYTVLEGDTIFGIAEKFGLNPETVLWGNYYVLLDNPHSLRPGQELNI
                     LPVNGTYHEWQQGEGLNGVSEYYKVKPEDIINYPPNNLDMATVGDFSNPNIPPGTWLI
                     VPNGRRDFVSWSAPLGVTRENPAFARVLGPGACDPVSGGAVGYGTFVWPSNSHLLSGY
                     DYAPAANHWGIDLAGNEGEGVYATDAGVIVYAGWNNYGYGNMILVDHGGGFQSLYAHL
                     SSINVGCGQSVGQGDVIGAIGNTGNSSGSHLHFEIMTSSYKMNPFDVLPPP"
     gene            6694..6767
                     /locus_tag="VFQ23_24720"
     tRNA            6694..6767
                     /locus_tag="VFQ23_24720"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6728..6730,aa:Val,seq:cac)
     gene            complement(6888..8183)
                     /locus_tag="VFQ23_24725"
     CDS             complement(6888..8183)
                     /locus_tag="VFQ23_24725"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET9909875.1"
                     /translation="MTTASFLDRAIAVLRGNDTGIFVKPGPHQYPHQWNWDSALIALG
                     LSHFDMPRAQLEIRSLLGGQWQDGMLPSIVYHSTPSDYFPNPEFWQIENSSSAPHVAT
                     TGITQPPLLATVVRTMHSRHSMPDFVREVYPSLLNWHRWMHTARDVDNTGLACIIHPW
                     ESGTDDSPRWLKLLASIKPRVVPEFQRGDTRHVSANERPHRSEYERFIYLIDVFRKLN
                     YAPTELLSNSPFLVQDVLFNAILFRADEDLRALAVSHNQPTNEIDSWIRKVQLNFNTR
                     FWHDGVGLYYDYDVRSNRLIQVNTASSFLPLFAGLPSKRQAQRLVEEHLLDPNEYAPS
                     GAARHWVTTTAKTEPTWEARRYWRGPVWVIMNWLIIDGLKRYGYEDIADTIRRNTIGL
                     IEASGFREYYDAHDGSGCGSTDFSWSAALALELSMDSSQ"
     gene            complement(8218..9318)
                     /locus_tag="VFQ23_24730"
     CDS             complement(8218..9318)
                     /locus_tag="VFQ23_24730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9909876.1"
                     /translation="MADIRIENVTKRFENFVAVDNVSLTVEDQQFVVLLGPSGCGKTT
                     LLRAIAGLGMADEGRISIGDRDVTYLPPRDRKISMVFQSYAIFPHMTVFDNIAFGLKM
                     NNFEKKEIDKRVREAAELLHIEEMLNRYPSKMSGGQRQRVAVARAIAMKSEVLLMDEP
                     LSNLDALLRLEMRAELKRLLQEIKATTIYVTHDQIEALSMGDHIAVMKEGKIQQYDKP
                     ETVYDMPANRFVGSFIGTPPMNFMQAQVEPENSHVNVRVGNYKLRPSNVMQPVLHSYA
                     GKQILMGIRAENMETLAKPANDALQVEVLVVEPLGSQNLLTVKIGDDLVKVATHPTFR
                     VARGEPVWLRFPGEKIRWVDRDSGQLLIPPPE"
     gene            complement(9328..10218)
                     /locus_tag="VFQ23_24735"
     CDS             complement(9328..10218)
                     /locus_tag="VFQ23_24735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9909877.1"
                     /translation="MTSMVDRQPNVAQVSPEVKAIHRRMLRRQRLTRAFTYLVGILIS
                     LWVLIPILFITSMALTTPAEVRAYPKGVLPFIPLSTETMEFFLSSRGVIPGLRNSVVV
                     ALITLVLSTLIAAPAGYALSRFLFRGRDFFRLSILAVRAFPIVILSIPLAVVFLRTGI
                     YDSVYSLALMHTALALPTTILVLSSVFSSIPYELEEAAMVFGCSPIQAFRYVVLPLVM
                     PGIAASAIFTFVLSWNEVFAATILTIQNRTLPAQVLVTLNQSSEPYQFAGGFFMMIPS
                     LIFIFFIRRYLFNMWGQISK"
     gene            complement(10215..11606)
                     /locus_tag="VFQ23_24740"
     CDS             complement(10215..11606)
                     /locus_tag="VFQ23_24740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9909878.1"
                     /translation="MTIAPKTKGRKNINWTPYILILPSFLYLVLFFGWPMVQGLILAV
                     REERALMTVRSDAERNSIIAGQLPRGAQVEIIGQQGNPIPRQDAGQKDLLTEIWFRVR
                     GESTEGELIEGWTPERRIRVRETDVNGEPSAGTVRSILSSTADPLTKIHAEADEGSEV
                     VGQMEARTAVQILDQAVLEVWFQVRGENGSESVTGWVPSRFINPSEDGTRGRVEDGDT
                     GRFTTRYIQSMVNDRFFWPAVRTTLLLIVLIIPVQFVLAIIMALVIQAGLKGTGVFLY
                     IFAIALGVSDLAVGILWYSIFTQSGYLNSILQGLGLIDRPIIFLSADTRYWILIAVWL
                     AEVWRATAIVMVIVVAGMQAISREVLEASEVFGANLWQRVRYIILPLLRPSLQVALIL
                     RTILAFQVFAVVIVMSGGDIMTVLANETYRQYYDLRNFNVAAAYASFILFLSMITSLF
                     YLRAVRTQEEVVE"
     gene            complement(11696..13126)
                     /locus_tag="VFQ23_24745"
     CDS             complement(11696..13126)
                     /locus_tag="VFQ23_24745"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9909879.1"
                     /translation="MIRLNRFVTTLIVTAFVLTACGPQETQPPATEAPTEVMTEAPTE
                     APTEAPTEAPTEAPTEPATSEATVTPTLGPPAGDVSFFSTQFNIVEEGAKFRAILSEG
                     GNYDFTGSEEGPLLDLIRAGAETGQGQVDLVGALHGTFPSITDAMLNMTDVVDDLSAD
                     REFNQAYLETGLLGTDDYLYYVPWMQATYIMAAHKDALQYLPAGADVNALTWEQLAEW
                     CQTLMDETDGPKCGVPHAGLFHRFLEGFLWPSFTGGMVTKFKSAEAVSMLEWARDSLW
                     PTLHPQSISYEFMQEPLQSGEIWVAFDHVARLIEALNADPESFVTFPAPAGPMGRGYM
                     PVIAGLGIPNDAPNPEAAMALIDYLTQPETQAQVLRELAFFPVVGGVDTTDLPAGVAL
                     EAAAVDATVNAPDALPALIPVGVGARGGDINNIFRAAFDRVVIEGEDPATVLEQEGSN
                     LQAVLDDTGAACWAPDPVSEGPCQVE"
     gene            complement(13538..14791)
                     /locus_tag="VFQ23_24750"
     CDS             complement(13538..14791)
                     /locus_tag="VFQ23_24750"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET9909880.1"
                     /translation="MQQFPDLHHWLQKAKALLESNRIEIDGHSYTAPTLDLDDFGRRD
                     YANQFLWDSCFHAIIWRLIDHQKAQAELLSLLSHQVKDGPDAGMIPHCIYWHNGGGWL
                     WSQSDRSSLTQPPLIAFAAFMVFEYSKALEFLAAIYSQVEKYHDWFDRRRDPDHDGLV
                     CIIHPWEAGGDAPPRWDKLLDFDSYDPEVGRATRIKMVEKLIRYSYDPIALAQHHGFH
                     VESLEINGIRAADLEAMADIAMILDKPEEAAAWLRRVDAVRSGFRSRMIVDGLPYDLD
                     GMEEKPIPQANAGQFVTLFGGLPTYEQAQQLVQQLKQTNFWTAFPVTNTPTDSLEYEP
                     DHYWRGNVWPCLNWLIYQGLRRYGFQDVADELAIRSFALLEQSGFWEYYHPQTGKGLG
                     GQAFSWAAVMLDMIISAAPRLEKKA"
     gene            14977..16044
                     /locus_tag="VFQ23_24755"
     CDS             14977..16044
                     /locus_tag="VFQ23_24755"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9909881.1"
                     /translation="MGKVLVFISPRTVGFESYDDRPLEPHEVRLRTLYSGISAGTELT
                     AYRGSNPYLHKQWDPTRKLFIPGEQTTLTYPVSGWGYEEVGEVIELGEDVQDVSIGNI
                     VFGVWGHRTHHIVEESYARDRLQSTSLDPILGIFSQVGAISLNGVHDARLRIGETVAV
                     FGLGVLGQIVGQLSRKSGAKVIGIDRLEKRLEIALKNEAADIVLNARDHAVAEHIRSI
                     TENRGADVAIEVTGSTLALHQAIRSVAYSSRVVAMGFFQGESAGLFLGEEFHHNRVNI
                     VGSQISGTDPELTYRWNRLRLIQTFMHLQVEGLINLKPVVSHVIPFENAGEAFRILDE
                     EPENALQVVLDFRSNKDTNLL"
     gene            16041..17063
                     /locus_tag="VFQ23_24760"
     CDS             16041..17063
                     /locus_tag="VFQ23_24760"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9909882.1"
                     /translation="MSRHLLRTALLGCGSFAHRHARTLRQLNDEVEMVAFCNRTIEKA
                     VEFSRQYTNGQASVFADHHKMFEEVQMDAVIICLPPYAHSDEVDLAASRGINVFIEKP
                     IALTSEKGWQMVEAAEKFAIVTQVGFMFRFGEAVERLKMMIDSGEAGPVGLMSARYFC
                     NALHAPWWRQRSLSGGQLVEQVIHMVDLMRFLMGDATTIYSRQENLFHQEVADYTVED
                     VSATVLGFPQGGVGVIYATNGAIPGRWINDYRLVSKNLTADFSNANNATFHFTAHPDR
                     TPEIISSDRDFYLLEMQDFLTAIRTGQKTRTPLREGAKSLDLALAATRSAQLGSEVHL
                     SANDQS"
     gene            17150..17788
                     /locus_tag="VFQ23_24765"
     CDS             17150..17788
                     /locus_tag="VFQ23_24765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9909883.1"
                     /translation="MTTRTNKAWLSDLKSSGETREAALEDLRSVIQKGLPYALSRWLS
                     PDEPQFSSLVEEVTQETLLRVLDQIDSFEGRSQFTTWVHKIAVRIALTELRRKRWRDA
                     SLDELTENEDLPPPPGLLADPHAGPETSAERADMIVRVRRIIDEELTDRQRQALLLLG
                     VQDMPMEDAARKLKTNRNALYKLLHDARLRLKNRLSMEDINAHEVLALFEQK"
     gene            17823..18119
                     /locus_tag="VFQ23_24770"
     CDS             17823..18119
                     /locus_tag="VFQ23_24770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909884.1"
                     /translation="MNIKELVRRIRVSLGLQKELTNETVLGFLRVLEDVRSEELTCDE
                     IYAKLDQYVECEANTKGAAQLMPLIRQHLDTCPDCCEEYEALLDVVEKTDEDEK"
     gene            complement(18164..18673)
                     /locus_tag="VFQ23_24775"
     CDS             complement(18164..18673)
                     /locus_tag="VFQ23_24775"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HET9909885.1"
                     /translation="MSDLADFRAEKDDFFKHFPQSPLTPEQRSDFKGLQYFPENDDLR
                     LEIQVERFFEQQPMQMQTSTGGVQAYVRYGKFKFDVDGQPAELTIYQSENGYFLPFVD
                     SLAGQETYPAGRYLEPEPLPGDRFLVDFNVAYNPYCAYNEMWSCPITPAENRLKIPIR
                     AGEKLFEHE"
     gene            18791..19177
                     /locus_tag="VFQ23_24780"
     CDS             18791..19177
                     /locus_tag="VFQ23_24780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909886.1"
                     /translation="MSQKTVLIIEDEEDAADMFAEMMRVSGYRVLKTSSSTPALSMMA
                     AEKPDVVILDIMMPEISGLDILRQMRKNPELVQTPVVVVSAKSMPADIKLGMEAGASI
                     YLTKPVGFVELKEAIERAVGRSSTSS"
BASE COUNT         4995 a         4775 c         4708 g         4711 t
ORIGIN      
        1 gaattactca taaccaattc cttcattcct cttgaagaag gcactcccag cattacaagt
       61 gaggtgttgg tgctggcggc tccgtattgg aatgatttgc gaaacaaatt gcttaaggtt
      121 gttgattcct atcatcagac atatccattg cggcgtggga tccctcgtga agagctaaag
      181 agcaggctta aactaccatc acgcgttttc aacggcgtga tgaaaaaact gagtttggat
      241 caatcgctga cagaatactc tgcctacctc gcaaaacccg agcatgaaat aaggtttgat
      301 aaccgtcaag aatccagggt cggagccctg atgcagaaat ttgaacaaaa tccgtttagc
      361 ccgccaagta tcaaagagtg ccagtctgag gttggggaag aagtcttgaa tgctctggta
      421 gaaataaaag agttaatcct gctttcccca gacgtaattt tccgcaagca agattatgaa
      481 tttatgaaaa gtaaagttca ggaattgttg gtccaacgtg aaaaaatcac acttgccgaa
      541 gtgcgcgatc tatttaacac cagtcgcaaa tatgcccagg ccttcctgga acacctggat
      601 acagttggta tcacaagaag ggatggggat tttagaaggg taggaaaaca atgaacacgc
      661 tgaaagcgtg gctttcacca cctgtcttcg ataacgctga aaaaacacat caagcgtact
      721 tattacactt cattttgttg gcattaatcg caattccaat tccattcgtc acgtatttac
      781 tgattcgagc ccctgaagaa tcagacagag cactgatact cattgcagta tcggaaatca
      841 tacacatcat tctcttcatc attctcaggc gcggacatgt tcaaatcgct tctttcttgc
      901 aagtcattac aatctggcaa ttcctcgcga tcgcttcgat aaccagttcc agcatatacg
      961 gcgtggcgta tactctcggc aatgcaatgg tcatcaccat tgcgggaatt ctattaggtg
     1021 ggcgtggagc tcttgtactt acgttgctaa ttattctgga aggggcaata ctggtctacg
     1081 cagaagtaca gggttgggat ccgccagaca ttctggatga tgcgctgccc acatggatcg
     1141 tcagcgtggt tctatttgca gtcgttgcaa gtttgcagaa cctggctgcg cgtgaagtcc
     1201 gggcagcgct gaatcgtgcc cagacaagcg aggagcgcta tcgcttgatc tcagaggtta
     1261 cttcagacta tacattttca acagaattta atgcaaaggg caacatttct ggcttctggg
     1321 ccggcggggc ctttgaaaaa attaccggct atacaattaa ggaatactca aacctcgaaa
     1381 gctggcggtc ccgtttgcat cctgatgatt tcgaaaagga tgcacaaaac acactggatt
     1441 tgaaacaaaa ccgatctttc gtttcagatt tgcgtctata ccgcaaagat ggtgatctgc
     1501 gttggattcg aatttacgcc aatccggtct ggaaccagaa ggaagatcga ctcgcaagag
     1561 tcgtgggggc ggtgcaggat attactgagc aaagacgcgc tgaagaattg gaacaacgac
     1621 gccgtttaat gatggaaaag gtcatccaac ttgggaagca cgtgacagaa gtaagcgatc
     1681 tccgcacgac acttgagcga atttggcatg gagttcatga tgaattggat tttgaccggc
     1741 tggcaatctt tctgcataat cccaagaaaa acttcatgca aggaacgctg gggacgaata
     1801 atgacgggca aattgtagaa gaatgggagg aaagttttcc gattacagta ggcgacatgt
     1861 ttcaccgggt gatacagaaa ccggatggat ttcatttcac ccacaattac gatatcgaag
     1921 aatcagttcc tgaaggggac gagatgtacg gcgtaaagga cttcgccgcg gttgcgggat
     1981 gggcaagcga caaaccggtc gccatcattg tggcagacaa tcttccaagc ggacgatcta
     2041 ttaccgacga aaaattggag gcgttgcgtc tgtttggcgg ctacgcggga ctggccattg
     2101 aaaacgcgcg tcttcataca gcattgcaaa atgaactccg actacaaaaa gaagcagagg
     2161 aacgcgaagt tcatagacgt gccagattgg agcaagtgat cctgctgggg caacaggtga
     2221 ccaaagtaag cgacctgcgg agcacgcttg aaaaaatatg gaacgggatt cattttgaat
     2281 tgggattcga tcgccttgca atttttctct atgaccagga agccaacacc gtacatggca
     2341 catttgggac cgacaatgac ggtcggcttg tggaagagtg ggaatatgtg caaacactcg
     2401 agcagcaaaa accatcatcc ttcctgcgcg cgcttgagca gcccaatgga ttatttttca
     2461 cacgaagctt tggtattgat ttcgagatac cagaaggcca taaaatgcac aatgtgcagg
     2521 acttcgttgc cgtttccgcc tgggcaggtg aaaaaccagt tgccatcatt acggttgaca
     2581 atctgccttc cggcagatcc atcacacacg cgcaccttga atccttgcgg ttattctgcg
     2641 ggtatgccgg actagccatt gaaaatgcac ggcttaatga agcacttcag gatgaactgg
     2701 aacatcggca gactcttatc cgggagctgg aagcgaagaa tgcagaactg gaaagattta
     2761 cttatacggt ttcgcatgac cttaaatcgc cattggtgac cattacggga ttcctcggtt
     2821 atctcgaacg cgacgcgctg gacggtaacg tacagaaaat taggagcgac atagagcgca
     2881 tcgtaagagc tgcggaaaaa atgcaggctt tgctcaacga cctgcttgaa ttatcgcgcg
     2941 taggcagatt gatcaatagc cctcagaacg taccatttgg ggatattgtc aacgaggcgc
     3001 ttgaaaatgt gcgaggcagg ctggaaagca ataagattaa tgtgaaagtg cacgatgatc
     3061 tccccacagt tcttggagat aaagttcgcc tcgtagaggt cttacaaaac ctgattgaca
     3121 atgcggcgaa attcacccat acgagcccta accccagtat tgagattggt acatcaggct
     3181 tggaccagga agcaaatcat atttattttg tccgtgataa tggcataggc attcaacctg
     3241 aatttcagga gcgcattttt ggtttgttca ataagttgga tcaaaccgtg gaaggcacag
     3301 gcattggctt aaccctggtg aaacgaatta ttgaagtcca cggcgggaaa atttgggtag
     3361 aatcagagct gggtaaagga gccacattct atttcacatt acccggtagt aaaaacgagg
     3421 agtaactatg tgcggaagat ttacgttaac tattgaccct gccgaggcgc aggaaaattt
     3481 tagtaatttt acatttcccg aaaagtttgc accgagattt aatattgcgc cctcacaacc
     3541 gattctggcg attccaaacg acgagaaatt taccgcagac ttttttatgt ggggcttgat
     3601 acccatgtgg gccaaagacc caagcattgg gagccgtctc atcaacgcgc gcgcggaaac
     3661 actggcagaa aaaccggctt ttcggggaag tcttaaatat aaaaggtgtc ttatcctcgc
     3721 cgatggattc tatgagtgga aaagttccga tggtaaaaaa tccaaaacgc ctttcttcat
     3781 tcatatgaaa gaccgcaagc cattcgcttt tgcagggtta tgggactcat ggaacagccc
     3841 ggagggatcc cagttgaaga cctgcacgat catcacaaca gaacccaatg agctgatgtc
     3901 tctcctgcac aaccggatgc cggtcatcct gcatccacgt gattatgaca aatggctaaa
     3961 cccctcgcct cagactcccg atcagttaac gcccttgctc aaaccgtttc ctgcggaaat
     4021 gatggatgcc tacccggtca ctccactggt caacaaaccg gcaaacgaca ctcctgaact
     4081 ggtcgtacca gcccggtagg tttcacaacc tatttcaact tgatacataa aacccaaata
     4141 ttcttaaacc aacggctgcc cgcactggct tcgcgtgatt tcattttgtt cttggtgggt
     4201 caattgatct ctgtggtcgg gacatggatg caagccactg ccctgcctta tcttgcttat
     4261 cgaatttcag gtcgccctct ggacctcggc ttgatcgggt tttccactac tttgccaact
     4321 cttctgtttg cattgcctgc cggtgtattc gtagaacatt gggataaacg caaagcagta
     4381 atccttttac aggctctgat gtccattcag gcctttgggc tggcctttct tacctataca
     4441 ggtcaaatcc aaatttggca tattgtactg ttggcatttt tatacggctc tgcgatggca
     4501 gtggaggtaa cggcacggca ggcaatgctc attgagctgg caggtcgtga tgcattaccg
     4561 agtgccattg cgctgcagac gaccgctttc aatctgggtc gcatattggg tccactatgc
     4621 gccgcctggc tcatctcagt cactggtagt gagggcagcg tttttctcgt caatgggata
     4681 agcttcatct ttgtaatcgg cgggttattc ctggcccgga caggtttcaa aacgattgaa
     4741 gatacaaatc aggatcggaa tttaggaaac gaatttaggg aaggcatcgg ttacatccgc
     4801 agtgaagtgg ttgtaatgtc tgtaattatc atgtctgccc tggtggggtt cttcggaatg
     4861 ccattgattc aacaaatccc agccttcgcg cgcgatgtgc tgggaaccat ccaggataca
     4921 gaagccatcg tcgcaactcg taccagcaac ttatatgccg cacagggagc tggcgctttg
     4981 gtcgctgcat ttatcgcagc ctcgatgcat tcttccgaca aaaacaggat gcttttgctt
     5041 gggcaagtta cgtttatcct acccattatc gcgctgggat tcacttcaaa taccgcggtg
     5101 gctttgatcc tgttgacatt tatcggctgg ggaaccgtga ctcaactagt caccatgaac
     5161 acaattattc aaacgcgtgt accaaatggt ttgcgtggac gtgtgttcag tatatatttc
     5221 tgggcactac agggcgtcgc gccgttcgga agcattttca ttggctggat cgcccaaacc
     5281 tggaatgtgc cgctggcgtc cattatcagc ggcgcaattt gccttctagg cgtagttgcc
     5341 gtacggctca cctttaagaa tcgtcaaaca tcgtcggctt agcgtcaggt taacgttaag
     5401 taatgcgggt tgacgccgat ttttcgccct gatatgatgc tccacttgga gagtgcaagc
     5461 tgaatggata acaaatcccc cataaacaaa aatgtagatt caccgggttt tttctcgcgc
     5521 ctgatgcaga ccctggtcgg cctcggcctg ggagaatcga tgctgcgact ggggacaaca
     5581 gtattgtcgg tgatccttct gggtgtagtg atttggctgg tccggctgtt ctatgcgcag
     5641 acgccagcca gaaatgtcgc caacgcgctg gaagcggagc caaccgccgc cgtcgccgaa
     5701 atccaggata tctcatcagc cagtgtggat gactttggcg gcatccctcg cctggcacaa
     5761 gtccatacga tcatcccatc tcgtccgcga caggaaattg tcacgtatac agttttagag
     5821 ggcgatacca tttttgggat cgcggaaaaa tttggtctaa atcccgaaac tgtgctgtgg
     5881 ggaaattatt atgtgttgtt ggataacccc cactcgctca ggccagggca ggagcttaat
     5941 atcctgccgg tcaatggcac ctatcatgag tggcagcaag gcgaaggttt gaatggagtc
     6001 tcagaatatt acaaggtgaa gcctgaagat attatcaact acccacctaa caacctggac
     6061 atggcgaccg tgggtgactt ttccaatccg aatatcccgc caggaacctg gttgatcgtg
     6121 cccaatggtc gtcgtgattt cgtttcctgg agtgcccctc tgggtgtgac ccgcgaaaat
     6181 ccggcgttcg cgcgtgtgct gggtccgggt gcctgtgatc cggttagcgg aggcgcagtg
     6241 ggatacggaa cgtttgtctg gccatcaaat tcacatctcc tctccgggta cgattatgcc
     6301 cctgcagcca accactgggg cattgacctg gcaggcaatg aaggcgaagg cgtgtatgcc
     6361 accgatgcag gtgtaattgt ctacgccggt tggaataact acgggtacgg gaatatgatt
     6421 ctggtggatc atggcggcgg ctttcaatcg ctctatgctc atcttagcag tatcaatgtt
     6481 ggctgcggtc aaagcgttgg gcagggtgac gtgatcggtg caattggcaa taccgggaat
     6541 tcaagcggat cgcacttgca cttcgaaatt atgacgtctt cctataagat gaatccattt
     6601 gatgtgttgc cgccacctta aatccccact ctcttctcat cagagaggat tgcttgcgca
     6661 acaactttat ctcggatata atgccgtcac tcagggcgca tagctcagtt ggttagagcg
     6721 ctactatcac acagtagagg tccggggttc gagtccctgt gcgcccacaa agacgagtcc
     6781 cgtggctcgt ctttttcttt tttagtagat tttgccgcgc tgtaaggtat atactaattt
     6841 ctccccaagg ttcgaagttt aaattttttg gtacgaacga catggctcta ttgggaagag
     6901 tccatcgaca actcaagagc caatgctgcg gaccaggaga aatcggtgga accgcaaccg
     6961 gagccatcat gtgcatcata atattcacga aacccggatg cttcaataag accaattgta
     7021 ttccgccgaa tcgtatccgc gatgtcttca tagccatacc gctttaaacc atctatgatc
     7081 agccagttca taattaccca tactggtcca cgccagtaac gccttgcttc ccaggttggt
     7141 tcagtcttag ctgtcgtcgt aacccaatgg cgcgcggcac cagacggtgc atattcattt
     7201 ggatcaagca ggtgttcctc gaccaaacgc tgcgcttgtc gcttagaggg caatccagcg
     7261 aacaatggca ggaagctcga agccgtattg acctgaatga gtcggtttga ccgcacatcg
     7321 taatcgtaat acaggcccac accgtcatgc cagaatcgag tattgaaatt caactgaact
     7381 ttgcgtatcc agctatctat ttcattggtt ggctgattat ggctcacagc taaagcgcgc
     7441 aaatcctcat cagcccgaaa cagaatggca ttgaagagaa catcctgaac aagaaatggc
     7501 gaatttgata ataattcagt gggagcataa ttaagttttc gaaagacatc gatcagatag
     7561 ataaatcgtt cgtactctga ccgatgcgga cgttcatttg ccgagacatg ccgagtatcg
     7621 ccccgttgga attcgggaac gaccctgggc tttatggatg ccaggagctt cagccagcgc
     7681 ggcgaatcat ccgtcccgga ttcccaggga tggatgatgc acgccagtcc cgtgttatct
     7741 acgtctcgag ccgtatgcat ccatcggtgc caatttaata aagatggata gacttctcga
     7801 acaaagtctg gcatggagtg gcgcgaatgc atggtccgaa cgaccgtcgc cagtagtggc
     7861 ggctgtgtga tgccggtagt ggcaacgtgt ggcgcactag aactattctc gatttgccag
     7921 aattcgggat ttgggaaata gtccgatgga gtggaatgat agactatgga tggaagcatg
     7981 ccgtcctgcc attgcccgcc caagagtgaa cgtatttcta actgcgcgcg tggcatgtca
     8041 aaatgcgaaa ggccaagggc tatcaatgcc gaatcccaat tccattggtg aggatactgg
     8101 tgcggcccag gtttgacaaa aatccctgta tcgttcccgc gcaacactgc gattgcacga
     8161 tctaagaatg aggctgtcgt catacgtgtt ctctagtgtt tatcttcatt caaatagcta
     8221 ttccggcgga ggaattaata actggccact atcacgatcc acccagcgga tcttctcacc
     8281 cggaaagcgc agccataccg gctcccctcg cgcgacgcgg aacgtgggat gggtagccac
     8341 ttttacaagg tcatcgccga tcttaacggt aagtaaattt tgtgatccta aaggttccac
     8401 cactaatact tctacctgca aggcatcatt ggcaggtttg gcaagtgttt ccatattttc
     8461 tgcgcgaatg cccattagga tctgcttacc cgcataggag tggagtacag gctgcatcac
     8521 attcgatggt ctcagtttgt aattgcccac tcgaacgttt acatggctat tctcaggctc
     8581 gacctgcgcc tgcataaaat tcatgggggg cgtaccaatg aagctgccaa cgaacctgtt
     8641 ggccggcatg tcatacacgg tctccggttt atcatattgt tgaatcttcc cttccttcat
     8701 cacagcgatg tgatctccca tgctaagagc ttcaatctga tcgtgcgtca catagattgt
     8761 cgtggctttg atttcctgca gcagccgttt taattcagca cgcatctcca atcgcaataa
     8821 ggcatccaga ttgcttagtg gctcgtccat caagaggact tcggatttca tggcaatcgc
     8881 ccgagcgaca gccacccgct gacgctgacc accactcatc ttgcttggat agcgattcaa
     8941 catttcttca atatgcaata actctgccgc ttcgcggact cgcttatcta tctccttctt
     9001 ttcgaagttg ttcatcttca acccaaaagc aatattgtca aaaacagtca tatgggggaa
     9061 gatggcataa ctctggaaaa ccatcgaaat cttgcggtcg cgcggcggca agtaggtaac
     9121 atcacgatca ccaatactga tccgcccctc gtccgccatt cctaatcctg caatggcccg
     9181 caacagggtg gtcttcccgc agccacttgg acccaacagt actacaaatt gctgatcctc
     9241 aacagtcaag ctcacgttat cgaccgcgac aaaattctca aagcgttttg ttacattttc
     9301 gattcgaata tcagccatat ctgcctctta tttggaaatc tgtccccaca tattgaacaa
     9361 gtaacgccgg atgaaaaaga taaagatcaa tgacgggatc atcataaaaa acccacctgc
     9421 aaactgatag ggctcagagg attgattcag agttactaaa acctgagcag gtagcgtccg
     9481 attctgaatt gtgagaatgg tggcagcaaa gacctcattc caggataaga caaacgtgaa
     9541 aatcgcggag gctgcgatgc ccggcatcac caatggcaac actacatacc gaaaggcttg
     9601 aatcggcgaa caaccaaata ccatggctgc ctcttccaat tcgtacggga tacttgaaaa
     9661 aacgctactg agcacaagaa tcgtggtggg caaggcgaga gcggtgtgca tcaaagccaa
     9721 actatacacg ctgtcataaa tacccgttcg gagaaacaca accgccaatg gaatggataa
     9781 gatcacaatc ggaaaggcgc ggactgccaa aatcgatagg cggaagaaat ctctaccgcg
     9841 aaacaggaaa cgggaaaggg catacccggc gggtgcggcg atcaacgtag agaggaccag
     9901 ggtaatcaag gcaacgacga cactgttgcg caggccggga ataacacctc ttgaactcag
     9961 aaaaaactcc atggtttcag tggataacgg aataaagggc aacacgcctt ttggataggc
    10021 gcgcacctcc gcgggcgtgg tcaatgccat ggatgtaata aacaggatgg gaataagaac
    10081 ccaaagcgag atgaggattc caaccagata ggtgaaggcg cgagtcagac gctggcgccg
    10141 caacattcgc cgatggatgg ccttcacttc tggagaaacc tgcgcaacgt taggctgcct
    10201 atctaccata cttgtcattc gactacctcc tcctgggttc gtaccgcccg caggtagaag
    10261 agtgaagtta tcatggataa gaacagaatg aagcttgcat acgccgcggc tacgttgaaa
    10321 ttgcggagat cgtaatactg tcggtaagtt tcattggcca acacggtcat aatatcgcca
    10381 ccgctcatca caattacgac agcgaaaacc tgaaacgcga ggatcgttcg caagatcaaa
    10441 gcgacctgca agctggggcg caggagcgga agaataatgt accggacgcg ttgccaaaga
    10501 ttcgcaccaa acacctccga agcttccaga acttcacggg agatggcctg catccccgcc
    10561 acgacaatca ccatgacaat cgctgtcgca cgccaaacct ctgccagcca aactgcaata
    10621 aggatccagt agcgggtatc cgcagagaga aagataattg gccggtcaat caaccccagc
    10681 ccctgcagga tcgaattcag atagccagac tgagtaaaaa ttgaatacca gaggattcca
    10741 accgccagat cagaaacacc caaagcaatg gcaaaaatat acagaaagac cccggtccct
    10801 ttaagtcctg cctggatcac aagtgccata atgatggcta acacgaactg cacagggatg
    10861 atcagcacga tcaacagcag ggtcgtccta acggcaggcc agaagaaacg atcattgacc
    10921 atactttgaa tatatcgggt tgtaaaccga cctgtgtctc cgtcttcaac gcggcctcgt
    10981 gttccatcct ctgaaggatt tataaatcgg gacggcaccc agcccgtaac gctctcgctt
    11041 ccattttcgc cacgtacctg aaaccaaact tccaaaacgg cctggtcgag aatttggacc
    11101 gcagtgcgcg cttccatttg gcctaccact tcacttcctt cgtccgcttc agcatggatt
    11161 ttggtgagtg gatcggccgt gctggataag attgagcgaa ccgtcccggc gctcggctcc
    11221 ccgttcacat cagtttcccg tacccgaatt cgacgttctg gcgtccaacc ttcgatcagt
    11281 tcaccctccg tactctcgcc gcgcacccga aaccagatct ctgtcaatag atctttctgc
    11341 ccggcgtcct gccgtggaat cggatttccc tgctgaccga tgatttcaac ttgtgctcca
    11401 cgcggtaatt ggcctgcgat tatgctattg cgttctgcgt cggaacgcac tgtcataagc
    11461 gcgcgctctt ccctcaccgc caggatgagt ccctgcacca tcggccatcc aaagaacaaa
    11521 accaaataaa gaaaggatgg caagatcaga atatatggcg tccaattgat gttttttcga
    11581 cccttcgttt tgggagctat cgtcatgtga tcaccttaca agtttcagga agggttttcc
    11641 ctggcgcatg cgcgaagagt gagcatgcac cagggaacat taaccataaa ctatcttatt
    11701 cgacctggca ggggccttca ctgacaggat ccggcgccca acacgcggca cccgtatcat
    11761 caaggacagc ctgcaaattg gagccttctt gctccagcac agtcgcagga tcctcacctt
    11821 caatcaccac ccggtcaaat gcggcacgga aaatgttatt gatatcaccg ccacgcgcac
    11881 ctacaccgac tggaatcaat gctggcaaag catctggagc gttgacagtc gcgtcaacag
    11941 ccgcggcttc cagtgcaacg cccgccggta gatcggtcgt atccacacca cccaccaccg
    12001 ggaaaaaggc taactccctc aggacctgcg cctgcgtctc gggttgcgtc aggtaatcaa
    12061 tgagcgccat ggctgcttca ggatttgggg catcgtttgg aatacccaag cctgcaatca
    12121 caggcatgta gccgcgtccc atgggtcctg ccggagccgg gaatgtgaca aaactttcag
    12181 ggtctgcatt gagagcttca atcaaacgtg caacatgatc aaaagccacc catatctcac
    12241 ccgactggag tggctcttgc ataaactcat aactaatcga ttgaggatgc agtgtaggcc
    12301 atagactgtc acgtgcccat tccagcatag agactgcctc agcactcttg aacttcgtga
    12361 ccataccacc agtaaaggac ggccacagga aaccctcaag gaagcgatgg aaaagcccgg
    12421 catgtggcac gccacatttc ggcccgtcgg tctcgtccat cagcgtctga caccattcag
    12481 ccaattgttc ccatgtcagc gcgtttacat cggcgcctgc gggtagatat tgaagagcat
    12541 ccttgtgagc tgccatgatg taggttgcct gcatccacgg gacgtaataa aggtaatcat
    12601 cagttccaag caagccggtc tccagatacg cctgattgaa ttcgcgatct gccgacaagt
    12661 catcgacaac atcggtcata ttcagcattg catcggtaat agatgggaat gtgccgtgaa
    12721 gcgctccgac cagatctact tgaccctgac ctgtctcagc gccagctcga attagatcca
    12781 gtaatggacc ttcctctgag ccagtgaagt catagttacc tccctctgaa aggatggcgc
    12841 ggaattttgc accttcctct acgatgttaa attgagttga aaagaaactt acatcccccg
    12901 ctggaggacc cagggtcggc gtaaccgttg cttcggatgt tgcaggttcc gttggggctt
    12961 ccgttggagc ttcagttggc gcttcagttg gcgcttccgt gggggcctcg gtcattacct
    13021 cagttggggc ttccgttgca gggggctgcg tttcctgagg tccacaagct gtcagcacaa
    13081 aggcggtaac tataagagtt gttacaaagc gattgagtct tatcatctaa ttcttctcct
    13141 tcgttccttc gccacttaat aagcgaacgg actatataca gaatcggata aatattttat
    13201 tttgcgaaga acacctcctt atgcgattca agttcggcat gcaacgccaa catccacctc
    13261 ttggagctac atctggtgtt gtgctagcat cgtgattgat gcaacgtcta tgtttagaag
    13321 ccctgagact gagtgtgaca gttacacatc tatgacaaat ctttaccccc tgcgataaac
    13381 ctttaattcg caatttcatt atagcgagaa actatgttcg agtcaatttg ttttggagtt
    13441 ggtttcggaa atttatcatc ttcttctcca tcatacggag accaaaccat tcactttaac
    13501 tccagctatt ctttcatcaa ctggccatcc agagacgtta tgcttttttc tccaagcgag
    13561 gcgcggcaga gataatcata tccagcatca cagccgccca gctgaatgcc tgcccgccca
    13621 atcccttccc ggtttgaggg tggtagtatt cccaaaagcc agattgctcc aatagtgcga
    13681 aactacgaat agccagctca tctgcgacat cctggaaacc ataacgacgt aaaccctgat
    13741 aaatcaacca gttaagacag ggccatacat tccctcgcca ataatgatca ggttcatatt
    13801 cgagcgaatc ggtgggtgta ttcgtaacgg gaaatgctgt ccagaaattt gtctgcttaa
    13861 gctgctgtac caattgctgg gcctgttcat aggtgggcaa gccaccgaat agggttacga
    13921 attgtccagc gttggcttga gggatcggct tctcctccat accatccaaa tcatacggca
    13981 gaccatcaac aatcattctg gagcgaaatc cagatcggac agcgtcgact cgacgtagcc
    14041 atgccgcagc ttcctcaggt ttgtccagaa tcatagcaat atcagccatt gcctccaggt
    14101 cagctgcgcg gatcccattt atttccagag actcaacatg gaagccatga tgttgagcca
    14161 gggcgatcgg atcatagcta tagcgaatca gtttctccac cattttgata cgcgttgcgc
    14221 ggccgacttc gggatcataa gaatcgaagt ctaacaattt atcccagcgg ggcggcgcat
    14281 cccctccggc ttcccacgga tgaatgatac ataccaggcc gtcatgatca gggtcgcggc
    14341 gtcggtcgaa ccaatcatga tatttttcca cctgcgaata gatagcagcg agaaattcga
    14401 gagccttgct gtattcgaat accatgaaag cagcaaatgc gattaacggt ggttgtgtga
    14461 gggagcttct atccgactgg ctccataacc agccaccgcc attgtgccaa tagatgcagt
    14521 ggggaatcat gcccgcgtcc gggccatcct tcacctggtg cgacaataag ctcagtaatt
    14581 cagcctgtgc cttttgatga tcaattaagc gccagatgat cgcatggaaa cacgagtccc
    14641 acaggaattg attcgcataa tccctccggc caaaatcgtc gaggtccaac gtgggcgcag
    14701 tatacgaatg cccatcaatc tcaattcgat tgctttctag aagagctttg gctttttgga
    14761 gccaatgatg caggtcaggg aactgttgca attcgacgat ctcctgtgct tcggtttgat
    14821 acagaaagaa acattgactg aattgattct actatgcact gaaagtgcca ggctatcggg
    14881 cgttataatt aagattgcgt tatagggtgt gacagatagc atgtcatcgg tcaaaataat
    14941 ctcaaatctt ggcaccctct tctggagtaa ttcggtatgg gaaaagtcct cgtattcatc
    15001 agcccaagaa cggtcggctt cgaatcgtat gatgaccggc cacttgagcc acatgaggtc
    15061 cgcctgcgga ctttatattc aggcatcagt gccgggaccg agcttacagc ctatcgcggc
    15121 agcaatccat atttgcacaa acagtgggat ccaacgcgca aactttttat tcccggggaa
    15181 caaacaacgc ttacttatcc ggtcagcgga tgggggtatg aagaagtggg tgaagttatc
    15241 gaactcggag aggatgtcca ggatgtgtcg atcggcaata tcgtctttgg cgtctgggga
    15301 catcgcacac atcatattgt agaagaaagt tatgccaggg atcgccttca gtctacgtca
    15361 cttgacccga tcttggggat cttttcacag gtgggtgcga tctccctgaa cggtgtgcat
    15421 gacgcgcgcc ttcgcatcgg tgagactgtg gccgtgtttg gtttgggagt cctgggtcag
    15481 attgtcggtc agctttcacg aaagtctggc gcgaaagtta ttggaattga tcgccttgaa
    15541 aagcgtttgg aaatcgcact caaaaatgaa gcagccgata tcgtattgaa cgccagagat
    15601 cacgcagtgg ccgagcatat tcgatccatc acagaaaatc gcggtgcgga tgtcgcgatc
    15661 gaagtaactg gctcaacgct tgctctccac caggcgatcc gttccgttgc ttattcgtcc
    15721 agagtcgttg cgatgggctt ctttcaggga gagtccgcgg ggctgttcct cggagaagag
    15781 ttccaccata acagagttaa tatcgtgggc tcacagattt ccggcacaga tcctgaactg
    15841 acttatcggt ggaatcgtct tcggctgatc caaacgttca tgcacctgca ggtcgaagga
    15901 ttaatcaatc ttaagccagt agtgtctcat gtgattccgt ttgagaatgc aggagaggca
    15961 ttccgcattc ttgatgaaga gcctgaaaac gcgctgcagg ttgttttgga tttcagatct
    16021 aataaggata caaatctgct atgagccggc acttactacg aacagcactt ttgggttgtg
    16081 gaagttttgc gcatcgtcac gcgcgcactc tacgacaact aaacgacgaa gttgagatgg
    16141 tggccttttg caaccgcacg attgagaaag cagtggaatt ctccaggcaa tacacaaacg
    16201 gtcaggcatc cgtatttgcc gatcatcata aaatgttcga agaagttcaa atggatgcgg
    16261 tcatcatctg cctgccccct tatgctcatt ctgatgaggt agaccttgca gccagccggg
    16321 gaataaatgt tttcatagaa aagccaattg cgcttacatc agaaaaaggg tggcagatgg
    16381 tagaggctgc cgaaaagttc gcgattgtca cgcaggtcgg atttatgttt cggtttggag
    16441 aagctgtaga acgcctgaaa atgatgattg attcggggga agccggtcca gtaggtttga
    16501 tgtcagcgcg ttatttttgt aatgctttgc atgcgccctg gtggcggcag cgttcgcttt
    16561 ctggagggca gttggtcgag caagtcattc acatggtgga tttaatgcgg ttcctgatgg
    16621 gagatgcgac gacgatttat tcacgtcagg aaaatttatt tcaccaggaa gtggctgatt
    16681 acactgtgga ggatgtaagt gcgaccgtgt tgggatttcc ccaaggagga gtaggtgtca
    16741 tttacgccac caacggcgcg atccctggcc gctggataaa cgactaccgc ctcgtttcaa
    16801 aaaaccttac cgcggacttc agcaacgcta ataacgccac atttcacttc acggcacacc
    16861 ccgaccgtac ccccgaaatc atctcctctg atcgcgattt ctatttactc gaaatgcagg
    16921 attttttaac ggcgatccgt actggtcaaa agactcgcac gcctttacga gagggagcca
    16981 aatccctgga tctggcactg gctgccacgc gctcagccca attgggcagt gaagtacact
    17041 tatccgccaa tgaccaaagt taaaacttat ctcggtggaa tattcaactc tcgcccacgg
    17101 atggtctata cgacatagag atggttttat gttttataat tccaactcta tgacgactcg
    17161 taccaacaaa gcctggctct ctgacttaaa gtcttccggt gagacgcgcg aagccgcact
    17221 cgaggactta cgttccgtta tccaaaaagg tctaccttat gccctctcgc gttggctatc
    17281 gccggatgaa ccccaattta gttcactcgt cgaagaggtg actcaggaaa cgctcctgcg
    17341 tgtcctcgat caaattgata gctttgaagg gcgaagtcaa ttcaccacct gggttcacaa
    17401 aatcgctgtg cgcatcgcac ttacagaatt acgtcgcaag cgttggcgcg atgcatcgct
    17461 ggacgaactc acggaaaatg aagaccttcc ccctccccct ggtttgctgg ctgatcctca
    17521 cgcgggacct gaaacgtccg ccgaacgggc ggacatgatc gtgcgcgtcc gcaggatcat
    17581 cgacgaggaa ctcacagacc gtcaacggca ggcattactt ctgcttgggg tacaggatat
    17641 gcccatggaa gacgccgcac gcaaactcaa aaccaatcgt aatgctctat acaaattact
    17701 gcatgatgcc cgtctcaggc tgaagaaccg cctctcgatg gaagatatca atgcgcatga
    17761 agtacttgcg ctctttgagc aaaagtaaga ttaagatatg attaaccatc aaaaccagtg
    17821 caatgaatat taaggaactt gtccggcgga ttcgtgtaag tcttggattg cagaaagaac
    17881 tcaccaacga gactgtcctc ggttttttgc gcgtcctgga agatgtgcgt tcagaggaac
    17941 tcacttgtga tgagatttat gcaaagcttg atcagtatgt ggaatgtgag gccaacacaa
    18001 aaggtgcggc gcagttaatg cccctgatcc ggcagcatct ggatacatgc cccgactgtt
    18061 gtgaagaata tgaggcactt ctggatgtgg tcgagaaaac cgacgaagat gaaaaataaa
    18121 acgacgagca cttgctcgtc gtttttgatt cgactctaag gaatcattcg tgttcaaata
    18181 acttttcgcc cgcacgaatg gggattttca gtcggttctc ggcaggcgta attgggcagg
    18241 accacatctc attataggca cagtaggggt tataagccac gttgaaatcc acaaggaaac
    18301 gatcgccagg caaaggttcc ggttcgagat aacgccctgc cggataggtc tcttgtccag
    18361 ccagtgaatc gacaaacggc aggaaatatc cattttcact ctggtaaatc gtcagttcgg
    18421 caggttgtcc atccacatca aacttgaatt tcccataacg gacataagcc tgcacgcctc
    18481 ccgtggaggt ctgcatttgc atgggttgct gctcaaaaaa cctttccacc tggatttcca
    18541 aacgcaagtc atcattctca gggaagtatt gcagaccctt gaaatcactt cgctgttcag
    18601 gcgttagtgg gctttgtggg aaatgcttga agaaatcatc cttctctgcg cgaaaatcag
    18661 ctaaatcact cataaatttt tttaatcctc cttgtcttag actgaaaata cttctcattt
    18721 cttacactcg gatatcgaaa tccacaagcc tcttgcatat tcattcctct tatgctatcg
    18781 ttatttttgt atgtcacaga agacagtgtt gattatcgaa gatgaggaag atgccgctga
    18841 catgttcgca gaaatgatgc gagtcagtgg atatcgcgtg ctgaaaacat ccagtagcac
    18901 ccctgcgctc agcatgatgg cggctgaaaa accagatgtg gtcatcctgg atattatgat
    18961 gcccgaaatc tccgggctcg acattttacg ccaaatgcgt aaaaaccctg agctggtaca
    19021 gacgccggtg gttgtcgtct ctgccaagag catgcctgca gacataaaac tggggatgga
    19081 agccggcgcg tcgatttact taacaaaacc agttggtttt gtcgaattga aagaagccat
    19141 tgagcgtgcg gttggtcgct cctctacgtc ctcgtaatac atgagttta
//
LOCUS       DASTJP010000853         9111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1139207, whole genome shotgun sequence.
ACCESSION   DASTJP010000853 DASTJP010000000
VERSION     DASTJP010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139207"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1208
                     /locus_tag="VFQ23_24785"
     CDS             24..1208
                     /locus_tag="VFQ23_24785"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9909887.1"
                     /translation="MKNLNQSAKSEKKIKVLIVITRLTIGGDTNVVLDIADYLNSHPP
                     YEVHMAVGPVLTHEVDLTYLAYEHKIPTTVIPNLVNYIKPWVNLKALYELYTLIRREK
                     FDIVHTHSSVGGVVGRLAAFLARVPVIVHHVHGWGIQENMSTTVRTLYLTLERICALF
                     TDRLIAVSAPTVQKGLAYRICKEDKFALIYNGINLQKFRQPVDRQKVFSDLGLDPDCK
                     IVGMIARLDQQKNPLDFIRAAAVVVKKYPKVQFLIAGDGSLRAECESLIDQLGLRGTF
                     FLLGYRDDINKIMPILTLTVLSSLWEGLPVVFQEAMSAGKPTVANDVDGARDVIIDGK
                     TGYLVTPHRYEEMAERILYLLNNEAVCHEMGLTAQNYSERFSGEKMVENITSLYRELL
                     KS"
     gene            1639..3750
                     /locus_tag="VFQ23_24790"
     CDS             1639..3750
                     /locus_tag="VFQ23_24790"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9909888.1"
                     /translation="MSSQAYPNQSELSRDVPSFDFNFFYSKGRKLAKRVFDFTMALIG
                     LIILAPVFTYVAILIKRDSPGPIFFWGSRMGRGGKPFKMLKFRTMFERAESYQGPPVT
                     ASGDSRITPLGQWLRDTKINELPQLWNVLIGEMSLVGPRPEDVEISKSWPKDAFDEIL
                     SVRPGVTSPASILYHDEEKLLSRANVMGDYLKGILPNKLRLDRLYVRHCSFFSDLDVI
                     FWTLAIILPRVSNTKIPEGYLFAGPFSRLIHRYVNWLLIDLLITTIVAYIVAIFSWGI
                     TEPLIVSIEALAFPLIFTALFTVINSLIGLNRVFWSQATIDDALGLFVSSGIATTLIW
                     IVNYLRYFYQWLPFSPLPSNMIIAIGIVSGFGFVAARYRLRLLTSLATRWLNWRQEAS
                     RVGERVLIVGLGDGNKIAHYLLKQRTFRTAFSIVGVVDNDNPSQHGMRVSGSWMLGGI
                     SDIPDLIERYDIGVVLSTIPHHKPENEHILEFCQLSRTRLIFLDDLLSMVDSQVTRPL
                     GKVDSQLWLDGRLEFKAMHDLLTGLPNRSLFQDRVQHTFAHAKRYKTQPALIFIEFDD
                     LAKISDSLGDKIGNEVLKDLGKRLISCKRESDTLARFGFDKFALLLENVPGDAELEIV
                     TQRITTALSEPLGINGQKYLLAPTIGACICQQTCGAFDVPGKVEIPRCYGCAMSKGIA
                     QRREMLAGVETLQEADSISSD"
     gene            3843..4817
                     /locus_tag="VFQ23_24795"
     CDS             3843..4817
                     /locus_tag="VFQ23_24795"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9909889.1"
                     /translation="MKILVTGGAGYIGSTLVPILLASGHSVRVLDNLMHGGRSLLGVW
                     PDLNFEFYEGDVRNEEHVRTALKDVDAVIHLAAIVGDPACARQPEVARATNFDASIQL
                     FNLSKESGVKKFVFASTCSNYGKMKDGSEYVDETSELSPVSLYAETKVAVEKYLLDPA
                     NSGSLSPTVLRFATAYGVSPRMRFDLTVNEFTMEILTKRKLVIFGEQFWRPYVHIRDI
                     SRAILCILESPTEKVQNEVFNVGSTTENFQKQQLADMLKSQIPDAVIEYVHKDEDPRD
                     YRVSFEKIKQTIGFNAEHTVGNGIQEVIRLVESGIIQDFKSPEYRNIS"
     gene            4900..5613
                     /locus_tag="VFQ23_24800"
     CDS             4900..5613
                     /locus_tag="VFQ23_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9909890.1"
                     /translation="MKAVILAGGKGTRLAPYTKVLPKPLMPIGDMPILEVILRQIKLT
                     GIDDVILTVGHLSELLRTFFQDGSRLGLNISYNYEDSPLGTAGPIAFVPNLDDTFLVM
                     NGDVLSTLPLKELIEFHKTNNAIATIATHHRRVKVDLGVIQWDGDARVTGYIEKPTYD
                     YSVSMGIYVFEPKVLTYIPKGEYYDFPDLVKKLIGVGEKVIGYRFDGYWQDLGRPDDY
                     ESAAQDFDQMRSQFLPNEV"
     gene            5617..6753
                     /locus_tag="VFQ23_24805"
     CDS             5617..6753
                     /locus_tag="VFQ23_24805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9909891.1"
                     /translation="MNWRVPLSDIDFDEAESLAVEKVLKSRWLTMGKVTQEFEAAFAA
                     HVQSKHAIAVTNATAALHLACLALGLGPGDEVIVPSLTFVATANAVRYVGATPVFADI
                     VGDDNLNISPESISALITPQTRAIIVVHYAGYPCDMEAILSIAKQHGLFVIEDAAHAV
                     GSELNGRKLGVWGDIGCFSFFSNKNMTTGEGGMMTTDNDELAQKLNRLRSHGMTSLTW
                     DRHKGHAYSYDVIDLGYNYRIDEIRAAIGLAQLSKVERNNERRRLLTQVYRDALQELA
                     PQVNVPFQHHLGKTSAHIMPVLLPSETKRQEFIQGMKENGIQTSIHYPPIHTFTSYKD
                     DAVWNLPVTEDIAKREITLPLYPAMSNDDVIVVVSAIRASLNLS"
     gene            6813..9062
                     /locus_tag="VFQ23_24810"
     CDS             6813..9062
                     /locus_tag="VFQ23_24810"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET9909892.1"
                     /translation="MNDFVELRRVMAIVLRRWWWLVIATILAALLGNIISRQQTPVYQ
                     ATTTILIGDSIKSPNVDRVDIQVSEALVQTYVEIARRQPVLQGVITTLNLNQSWQSLN
                     NQIQVTLIDLTQLIEIVVEANSPEMARNIADEIVNQMILLNPASVSDINSEAVNTFNR
                     EQLQSLQQRIVDGQNRLAEIDTAMSTSISEIELDNLQREKATLQSLIVEWEKNYTQLL
                     VITEPKRNPTQITVIESAHSSDRPIRPRTQLNTLLSGLVGLILALAVVFLLDFLDDTY
                     KSYSDFSLSEEVNLLGSIRKIKGRKLADKVVAHTHPYSPVTEAYRIIRSRIRFGPSTS
                     SARSIMVTSSMPAEGKSITVANLAVVFAQANYKTLVVDADLRHPVLHEMFNVSNEAGL
                     GDLLSSTELTVQDCIKDTSVSNLQILTSGTPLLDPSERLGSKRMEEILSELKTLAEVV
                     ILDSAPVLVFADAIVLSRRIDGVIVVIQAGKTKRSAITQAFFDLQNANAHLLGSIFNQ
                     SPQSDTFSVNKAYMQERPQLPSARTLKRKENTGSFKDLREAASPLSGSMALQKAIEDT
                     DPSPDQAELEELEFETQQATIAEEEIADFVETAKPDYDEPAMDEQTEEAAHPMSFSSE
                     NEDTRVIEDAHVAEAVEEVELELQDEIADLHLEDSSLEESVELETNETPGEEMHPSNA
                     SGASISEYTNKKSHKHRSRKNRHNGHLRASIAGENNFPKNGKTHVVTNESSNTSTDQA
                     TIQVDRTEE"
BASE COUNT         2474 a         1954 c         2161 g         2522 t
ORIGIN      
        1 tttaagaagc ctatcaaagt attatgaaaa acttgaacca atcggcaaag tccgaaaaaa
       61 aaattaaagt attaattgtg atcactcgat tgaccatcgg cggggatacc aatgttgtgc
      121 tggatattgc agattacctc aacagccatc ccccctatga ggttcacatg gctgttggac
      181 ccgttctcac gcatgaagtt gatctaacgt accttgccta tgaacataag attcccacaa
      241 cggtcatccc aaatttggtt aattatataa aaccctgggt aaaccttaag gctctttatg
      301 aactgtatac gctaatcaga cgggaaaaat ttgatattgt gcatacccat tcttcagtgg
      361 gaggggtggt agggcgcctg gcggcgtttc ttgctcgtgt ccccgttatt gttcatcatg
      421 ttcacggctg ggggatacag gaaaatatgt ccacaacggt gcgaacatta tatttaacat
      481 tggaaagaat atgcgctctc tttacggatc gattgatcgc cgtttccgcg ccgacagttc
      541 aaaaaggact tgcctaccgc atatgtaaag aagataaatt cgctctgatc tataacggta
      601 tcaatttaca aaaattccga cagccggttg accgacagaa agtgttttct gacctggggc
      661 tggatcctga ctgtaagatt gttggcatga ttgcacggtt agatcaacag aaaaatcccc
      721 tggattttat ccgggcagca gcggttgttg taaagaaata tccaaaggtt caattcttaa
      781 ttgcgggtga tggctcacta cgcgctgaat gcgaaagctt aattgatcaa ctgggtttac
      841 gaggtacatt tttcctttta ggctatagag atgacattaa caagatcatg cctatcctaa
      901 ctctcactgt gttaagctct ctttgggaag ggctgccggt cgtctttcag gaagctatga
      961 gcgccgggaa gccaacggtt gccaatgatg ttgacggtgc ccgcgatgta attattgatg
     1021 gaaagaccgg ttatttagta accccgcatc gatatgaaga aatggcagaa cgaatcttgt
     1081 acttgcttaa taacgaggct gtttgccatg aaatgggact gacggcacaa aactattcag
     1141 agcgtttttc tggcgaaaaa atggtggaga acatcacatc cctttatagg gaactcttaa
     1201 agagctaaat gaattcaact ttactcgttg ttaatgtgcg gaggcgttct ttcgacctga
     1261 ttttcggata aattcgtcaa ttttggggaa cgaaaaccct gtttacaact tgttaataag
     1321 agagggaatg tcaaatagat atacgaaacc tcaatgtaac cttttaactt ttttgatgtc
     1381 atgtactaac aaatctgcaa aaagaactaa taagacggta atcgcgctca actacgagaa
     1441 cggccgggca atttctgatg tctctcccgc tcttgtagtg ctgaggagtt cgatatggta
     1501 aaggaaatgt cttaccgtca ggcatccctt tgtggatttc tttatcgtac ctcacaccgc
     1561 atacctgagc ttaataaatc ttttggcaac acgatattat tcatttagta gatcatctgc
     1621 tgctattatc gaggaaatat gagttcacaa gcttatccta atcaatcaga actctcgcga
     1681 gatgttcctt cctttgattt taattttttc tattcaaagg gtcggaaatt ggctaaacga
     1741 gtctttgatt tcacgatggc actcattggg cttattattc tcgcgccggt ttttacttat
     1801 gttgccatat taatcaaacg cgactctccg ggtccaattt tcttctgggg ttcgcgcatg
     1861 ggccggggtg gaaagccatt caaaatgctg aaattccgca ccatgtttga gcgggcagaa
     1921 agctatcagg gtcctccggt aacggcaagt ggtgactccc gaattacccc tctgggacag
     1981 tggttacgtg atacgaaaat aaatgagctg cctcaacttt ggaacgtcct aatcggagag
     2041 atgagcctgg ttgggccgcg accagaagat gtagagattt ccaaatcctg gccaaaagac
     2101 gccttcgatg aaatactttc cgtcagaccg ggagtgacca gcccggcgag tattctttat
     2161 catgatgagg aaaagctgct ttcccgcgcc aatgtcatgg gcgattatct gaaaggcatc
     2221 ctgcctaata agttgcggct tgacaggctt tatgttcgtc actgctcatt cttctctgac
     2281 cttgacgtca tattttggac gttggcgatt atcctgccgc gagtctccaa tacaaagatc
     2341 cctgaaggat atctatttgc gggaccgttc tcgcgtctga ttcatcgcta tgttaattgg
     2401 cttctgattg atttgttgat cacgaccatc gttgcttata ttgtagcaat cttctcgtgg
     2461 ggcattaccg agccgttgat tgtgtccata gaggcactcg ccttcccact aatatttact
     2521 gccctcttta ctgttattaa ttccctcatt ggccttaatc gggtattttg gtctcaggca
     2581 acgatagatg atgctcttgg tttgtttgtt tccagcggta tcgcgactac gctaatttgg
     2641 atcgtaaatt atttgcgcta tttttatcaa tggctgccat tctcgccact tccctccaac
     2701 atgattattg ccattggcat tgtttcaggg tttggctttg tggccgcacg gtatcgccta
     2761 agattactta cctccctcgc cacacgctgg ttgaattggc gtcaggaggc atccagagtc
     2821 ggtgaacgcg tcttgattgt tgggctcggt gatggtaata agatcgctca ttacttgctc
     2881 aaacagcgaa cattccgcac tgcgttttca attgttgggg ttgtggataa tgataatccg
     2941 tcccagcatg gtatgagagt gagcggatcc tggatgcttg gaggaattag cgatattccg
     3001 gacttgatag aacgttatga tattggagtg gtcttatcaa cgatccctca tcataaacct
     3061 gagaacgaac acattcttga attttgccag ctctcgcgca cacggttgat ttttcttgat
     3121 gaccttttat ctatggtaga tagtcaggtg acgcgaccct tagggaaagt tgactcgcaa
     3181 ttatggcttg acggacggtt ggaatttaaa gccatgcatg atctccttac agggctgccc
     3241 aatcgctccc tgtttcagga tcgcgttcag cacacatttg cacatgctaa aagatataag
     3301 acgcaaccag cgttgatctt catcgaattt gatgacctgg caaagatttc cgattctctt
     3361 ggagataaaa ttggtaacga agtgctaaag gacctgggaa aacgcctcat tagttgcaaa
     3421 agagagagtg ataccctggc tcgctttggc ttcgataagt tcgcgttgct cctggagaac
     3481 gtgcccggtg atgctgaatt ggaaatagtc acccaaagaa ttaccaccgc attgtcagag
     3541 ccgcttggca taaacggcca gaagtatcta ctcgctccaa ccattggcgc ctgtatatgt
     3601 cagcaaacct gtggtgcttt cgatgttcca gggaaagtcg aaatcccaag gtgttacggc
     3661 tgcgctatgt cgaagggaat cgcacagaga agagagatgc tagctggcgt ggaaacgcta
     3721 caggaagctg attccatatc cagtgactaa tgcaatttag ttatggataa taatagcaat
     3781 actactaaat tcataaagta ggtttgatca aaaactacaa ttaggtgtaa atagaggttc
     3841 taatgaaaat acttgttact ggcggtgctg ggtatattgg ttcgactttg gtacccattt
     3901 tgcttgcttc tgggcacagt gttcgcgtcc tggataacct gatgcatggc ggcagatcgc
     3961 ttttaggcgt atggcccgat ctgaattttg agttttacga aggagatgtt cgaaacgaag
     4021 agcatgttcg cacagcactc aaagacgtag atgctgtgat ccacttggcg gcaattgtgg
     4081 gcgatcccgc ttgtgctcgc cagccagagg ttgctcgagc aactaatttt gatgcatcca
     4141 tccaattatt taatctctcc aaggaatcgg gtgttaagaa atttgttttt gcatccacct
     4201 gcagtaacta tgggaagatg aaagatggca gtgaatacgt ggatgaaacc tctgagctga
     4261 gtcccgtttc tttatatgcc gaaacgaaag tggctgttga gaaatatttg ttggatcctg
     4321 cgaactccgg ttcacttagt ccgacagttc tgcgctttgc cacggcatat ggtgtttcac
     4381 ccaggatgag gttcgatctg accgttaatg aattcactat ggaaattttg acgaaacgca
     4441 aattggtgat ctttggggaa caattctggc gtccttacgt tcatattcgt gatatttcga
     4501 gagcaatctt atgcattctc gaatcgccta cagagaaggt tcagaatgaa gtatttaatg
     4561 tgggctccac gaccgaaaat ttccaaaagc aacaattagc cgatatgttg aaatcacaaa
     4621 ttcctgatgc ggtcattgaa tatgttcata aggatgaaga tccacgggat tatcgggtgt
     4681 cgttcgagaa aataaagcag accattggct tcaacgctga acataccgtt ggaaatggca
     4741 tccaggaagt tatcaggttg gtcgagagtg gcatcattca ggactttaag agtcctgagt
     4801 atagaaatat atcttgacca aaattactca cgtaacccca catgtcttag ttaatcaaag
     4861 ggcgtttgag tgttacaaat tggatagtag gaactaaaca tgaaagccgt tatcttagcc
     4921 ggcggcaaag gcacccgcct ggctccttat acgaaggttc tacctaagcc tctcatgccg
     4981 attggcgaca tgcccattct ggaagttatt ttgcgccaga taaaacttac tggtatagac
     5041 gatgtgattt tgacagttgg acatctttca gagctacttc ggacattctt tcaggatggg
     5101 tcgaggttag gacttaacat ttcctacaat tatgaagatt cgccacttgg aactgccggt
     5161 cctattgcgt ttgtaccgaa tttggatgat acgttcctgg ttatgaatgg ggatgtgctt
     5221 tcaactctcc cactgaagga actcattgag tttcacaaga caaacaatgc aattgcgacg
     5281 attgctacac accatcgtag agtgaaggtt gatctcggtg tcattcaatg ggatggcgat
     5341 gcgcgcgtca caggatacat tgagaaaccg acttatgatt attccgtaag catgggaata
     5401 tatgtgtttg aaccgaaagt gttgacgtac attcccaaag gtgagtacta tgattttcct
     5461 gacctcgtaa aaaagcttat cggggttggg gaaaaagtga ttgggtaccg gtttgatggt
     5521 tattggcaag atcttggacg tcctgatgac tatgagagtg cagcacagga ctttgatcag
     5581 atgcgctctc aattcttgcc caacgaggtt taaaaaatga attggcgtgt tccgctttcc
     5641 gatattgatt ttgatgaagc tgaatctctg gcggttgaaa aggtattaaa gagccgctgg
     5701 ttgacaatgg gaaaagttac tcaggagttt gaagcagctt ttgctgccca tgttcagtct
     5761 aagcatgcca tagcagttac aaatgctacc gctgcgttac acctggcatg tctcgccctt
     5821 ggattaggtc caggagatga ggtgattgtt ccttccttga catttgtggc aactgctaat
     5881 gctgttcggt acgtgggagc tacgccggtg tttgccgata ttgtcggtga tgacaacctc
     5941 aacatttctc ctgagtcgat cagtgccctg attacacctc aaacgcgggc gattatcgta
     6001 gtacactatg ccggatatcc ctgtgatatg gaagccatcc tttccattgc aaagcaacat
     6061 ggcttgtttg taatcgaaga tgccgcacat gcggtaggtt ccgaactcaa tggtcgaaaa
     6121 cttggagttt ggggtgacat tggatgcttt agcttcttct ccaacaagaa tatgacgacc
     6181 ggagaaggtg gcatgatgac tacagataat gatgagcttg ctcagaaatt gaatcggttg
     6241 cgctcgcatg gaatgacttc tttgacctgg gatcggcaca aaggtcatgc gtatagctat
     6301 gatgttattg atttgggcta taattatcgg atcgatgaaa tcagggctgc aattggtctg
     6361 gctcagttat ccaaagtgga gcgtaacaac gaacgccgaa gactgttaac ccaggtgtat
     6421 cgtgatgctt tgcaagagtt ggctccgcag gtaaatgttc ccttccaaca ccacctcggc
     6481 aagacatctg ctcatatcat gccggtcctg cttcccagcg aaacgaagcg tcaggaattc
     6541 atccagggta tgaaagaaaa tggaatacaa acaagtatcc attacccgcc aatccataca
     6601 tttacatcct ataaggatga tgcagtttgg aacttaccgg taacagaaga tattgcgaag
     6661 cgcgaaatta ccttgccact ctatccggca atgagcaacg acgacgtcat tgttgtggtc
     6721 tcagccattc gggcaagctt aaatcttagt tgagttgttg agaagatatt gaagactcgc
     6781 tgcagtttct catgactaga aagtggtgtt aaatgaacga ttttgtggaa ttacgacgtg
     6841 taatggccat tgtcctacgg agatggtggt ggctggtgat agcaactata ttggcagctt
     6901 tgctaggcaa cattattagc cgccaacaga ccccggtcta ccaggcgacg acgacaatcc
     6961 ttatcggtga cagtataaag tcacccaatg tggatcgggt ggatatccag gtcagcgaag
     7021 ccctggtaca gacctatgtt gaaattgcca gacgccaacc tgtgttgcag ggtgttatca
     7081 cgacgctaaa tctaaaccag tcctggcaat ctctgaataa tcaaattcag gtcactctca
     7141 ttgatctcac gcaattgata gagatcgtag ttgaagcgaa ttctcctgag atggcgcgta
     7201 acattgcaga tgaaattgtc aatcaaatga tcctgttaaa tccggcaagc gtttcagata
     7261 ttaacagcga agctgtcaac acctttaacc gcgagcaact tcaaagctta cagcaacgaa
     7321 ttgtggatgg acagaatcga cttgcagaaa ttgacactgc aatgagcacc tcaatttctg
     7381 agattgaatt ggataacctt caaagagaaa aagcaactct gcaaagtttg atcgttgaat
     7441 gggagaaaaa ttatactcag ttactggtga tcaccgagcc gaagaggaac cccactcaaa
     7501 tcaccgtcat tgaatcggcg cattcgagcg ataggccgat tcgacctcgt acccaattga
     7561 acaccctgct gtcaggtctg gtgggattga tccttgcttt ggctgtcgta ttcctgttgg
     7621 acttccttga tgatacttat aagtcctata gtgacttttc tctatctgag gaagtgaacc
     7681 ttcttggtag catacgaaaa atcaaaggca gaaagcttgc ggacaaggtg gtcgcgcata
     7741 ctcatccata ttcgcctgtg actgaggcgt atcgtattat acggagtaga atcagattcg
     7801 gaccgtcaac gagttcagcc agatcgatca tggtaacaag ctcgatgcct gcagaaggca
     7861 agagcattac tgttgccaac cttgccgtcg tttttgctca agcaaactat aaaaccctcg
     7921 tcgtggatgc ggatttgcga catccagtgt tgcatgagat gttcaatgtt agcaacgaag
     7981 cgggactggg tgatctgtta agttcaactg aacttacggt gcaggattgc attaaggata
     8041 cttcagtcag caatttgcaa atactcacca gcgggacacc tctgcttgat ccttctgaac
     8101 ggcttggctc gaaacgtatg gaagaaatcc taagtgagct taagactctt gccgaggttg
     8161 tcattcttga tagcgcgccc gtcctggtat ttgccgatgc gattgttcta tcccgccgca
     8221 tcgatggcgt gattgtcgtg atccaagctg ggaagaccaa gcgttctgcc attactcaag
     8281 ccttctttga tctgcaaaat gcgaatgcgc atttgcttgg cagtattttt aaccaatctc
     8341 cccagagcga tacgttctct gtaaataaag cttacatgca ggaacgacct caattacctt
     8401 cggctcggac tttgaaacgg aaggaaaata cgggtagctt taaggacctg cgcgaagctg
     8461 cttcaccgct tagcggaagt atggcgttgc agaaagcaat tgaggataca gatccttctc
     8521 ccgatcaagc cgagcttgaa gagctcgaat ttgaaaccca gcaggctact atcgccgaag
     8581 aggaaattgc cgattttgtg gaaacggcaa aacctgatta tgatgagcca gccatggatg
     8641 agcagactga agaagcagct catcccatga gtttctcctc agaaaatgag gacacccgtg
     8701 ttatagagga tgcacacgta gctgaagctg tagaagaggt agagcttgaa ctccaagacg
     8761 aaattgctga tcttcacctt gaggattcat ccttagaaga aagcgttgaa ctcgaaacga
     8821 atgaaacccc tggcgaagaa atgcatccct ctaatgcaag tggagcgagt atttcagaat
     8881 acacaaacaa aaagagccac aagcaccggt ctcgaaaaaa cagacacaat ggacatttga
     8941 gagcctccat cgcgggtgaa aataattttc ctaaaaacgg aaagacccat gtcgtaacaa
     9001 atgagtcctc gaatacttcg acagatcagg ctacaattca agttgatcgg accgaagaat
     9061 aaatgcactg atgtactgct ccttggcaaa tttgtttgcg aaggagcagt t
//
LOCUS       DASTJP010000854         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1561248, whole genome shotgun sequence.
ACCESSION   DASTJP010000854 DASTJP010000000
VERSION     DASTJP010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561248"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /gene="deoC"
                     /locus_tag="VFQ23_24815"
     CDS             <1..871
                     /gene="deoC"
                     /locus_tag="VFQ23_24815"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET9909893.1"
                     /translation="AAMMEEQERGSQSDGYHCKFDCANQLCVKVCMDKAGNVVNAGAE
                     RLTSTLGVIPSDMSLAGMIDHTLLKPDATQQEIAQLCFEARKHNFASVCVNPTWVSLC
                     AQLLEGSPVKVCTVIGFPLGATTPETKAFETETAIKQGATEIDMVINIGALKARDLEL
                     VARDIRGVVNAAHARGIIVKVILETALLTDEEKTIASLISKEAGADFVKTSTGFASGG
                     ATVHDVALMRKAVGPQMGVKASGGVRTYEDAESMIKAGATRIGASAGVKIIQGPSNQT
                     GAKSEAGSSAKSY"
     gene            881..1171
                     /locus_tag="VFQ23_24820"
     CDS             881..1171
                     /locus_tag="VFQ23_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET9909894.1"
                     /translation="MLIARVIGTTVSTIKDEKLHGRKLLILRQTDEHGNVSGKPYVAV
                     DTVDAGVGDLVLTAAGSSARQTTITKDSPVDSVIMSIIDSLEVDGEVVFRKS"
     gene            1183..1566
                     /locus_tag="VFQ23_24825"
     CDS             1183..1566
                     /locus_tag="VFQ23_24825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909895.1"
                     /translation="MPKTFYTEKDIEELFKSGTRSLQVNDNVSLTDLAYERAKRLGMQ
                     LLFADVDLPPIAPIRPYLSNGSAQTPTASRTTPKVESVSPAPQPQTEVAQRIRSAVLE
                     RLGNQVDAKLLDSIIQRVVKATGVK"
     gene            1566..1886
                     /locus_tag="VFQ23_24830"
     CDS             1566..1886
                     /locus_tag="VFQ23_24830"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET9909896.1"
                     /translation="MLFGRVHGNAVCTIRYPGTEGVKLLVVQPLNKNMEPIGTLQVAA
                     DVVQAGPGDLCVMVRSREAALAMKDEKFVPIDLALVGIVDELEVKPDGTFDYILRAAQ
                     NYFT"
     gene            1929..2201
                     /locus_tag="VFQ23_24835"
     CDS             1929..2201
                     /locus_tag="VFQ23_24835"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET9909897.1"
                     /translation="MVLGKVVGTIVTTISHPHYKNRRLLVVQPLIVEGETADEDFIAL
                     DNTQAGIGDTVLVNREGNGARQALKNPDAAVISVIVGIVDSVHIEM"
BASE COUNT          688 a          589 c          722 g          636 t
ORIGIN      
        1 cgccgcgatg atggaagagc aggaacgcgg ctcgcaatcc gatggatatc attgcaagtt
       61 tgattgcgca aatcagcttt gtgtcaaagt ttgtatggac aaagcgggga atgttgttaa
      121 tgcgggagcg gagaggttga catccacgct tggtgtgatt cccagtgata tgagcctggc
      181 gggtatgatc gatcatacct tgctcaagcc ggatgctact caacaggaaa tcgcccagct
      241 ttgctttgaa gcgcgcaaac ataatttcgc ttcggtatgc gtaaacccta cctgggtgag
      301 tctgtgcgcg caactcttgg agggatcacc ggtcaaagtt tgtacagtga ttgggtttcc
      361 tttgggggct acaactcccg aaacgaaggc gttcgaaacc gaaaccgcca tcaaacaggg
      421 tgcgaccgaa attgacatgg tcatcaatat cggtgccttg aaggcacggg atcttgagct
      481 ggtggcacgg gatatccgcg gggtcgtaaa tgcagctcat gcgcgcggga ttatcgtcaa
      541 ggtgattctt gaaaccgcgt tactcacgga cgaagaaaaa acaattgcaa gtttaatttc
      601 gaaggaagca ggcgcggact ttgtcaaaac gtccacaggc tttgcaagtg gtggcgcgac
      661 ggttcatgat gtcgcgttga tgcgtaaagc agtcggccct caaatgggtg tgaaagcctc
      721 gggtggagtt cggacgtatg aagatgcaga aagcatgatc aaagcgggcg cgacacgcat
      781 cggcgcgagc gcgggagtaa agatcattca gggtccgagc aatcagaccg gggcgaagag
      841 cgaagcgggt tcatccgcaa aaagttacta ggagcttgag atgttaattg cacgagtcat
      901 tggcacgacg gtctccacca tcaaagacga aaaattgcac ggtcgcaagt tgttgatcct
      961 gcgtcagacc gatgagcacg gcaacgtgtc aggcaaaccg tatgtggctg ttgatacggt
     1021 ggatgcaggt gtgggagatc tggtattgac ggctgccggc tcctcggcgc ggcaaaccac
     1081 gatcaccaaa gactctccgg tggattcagt gattatgtcg attatcgatt ccctcgaggt
     1141 ggatggggaa gtggtgtttc gcaaatcatg ataaggtaac tcatgcctaa aacgttttac
     1201 accgagaagg atatcgagga actcttcaag agtggaaccc gatcattgca ggtgaatgat
     1261 aatgtttccc tgactgatct cgcttacgaa agagcaaaac ggttaggtat gcagctatta
     1321 ttcgctgatg tagatctgcc tccgattgcg cccatccgac cttatctttc gaatggatca
     1381 gctcaaactc caactgccag ccgcacgacg ccaaaagttg aatccgtttc acccgcgcct
     1441 cagcctcaaa cagaggtcgc acaacgcatc cgctctgcgg ttcttgagcg cttgggaaat
     1501 caagtcgacg cgaagctctt ggactcgatc atccagcggg tcgtgaaagc gacgggggtg
     1561 aaataatgtt attcgggcgc gtgcacggaa acgcggtttg cacgattcgg tatcctggta
     1621 cagagggcgt gaaacttctg gtcgtccagc ccctgaataa gaacatggag cccatcggga
     1681 ccctgcaagt ggcggctgac gtagtgcagg ccgggcccgg tgatttgtgc gtcatggttc
     1741 gttcgcgtga agcggcattg gcgatgaaag atgaaaaatt tgtgccgatt gatctggcac
     1801 tggtgggaat tgttgatgaa ctcgaagtga aaccagatgg tacgtttgac tacattttgc
     1861 gtgctgcgca gaattacttt acttagtttt taaccaccac gtgcacaaag gaacacaaag
     1921 gtttaagaat ggtcttaggc aaagttgtcg gaaccatcgt cacaaccata agtcatccgc
     1981 attacaagaa tcggaggctg cttgtcgttc aacctttgat cgtcgaaggc gagacagccg
     2041 acgaagattt catcgcgctg gataatacac aggctgggat cggggatact gtattagtca
     2101 accgcgaagg caatggcgcg cgtcaggctt tgaaaaatcc cgatgcagca gtaatctccg
     2161 ttattgtggg gatagttgat tcagtgcata ttgaaatgtg aacggtttca atccaagtat
     2221 gataattgaa taaagagact ccaagcttcg gatgttgctt atttttagat cccatgactc
     2281 tatgtcagag tacgctacca atcgtttcat aggtcgaaaa aattagatgc ctggcgctgc
     2341 ttcggccaac cttgtagaag gttgtgaagt attcacacat attgctgtac ttgcattctg
     2401 taccatataa ccccctttca ccctcgatta caatgtaaat cgatatagta aaatatcagt
     2461 tcgcagcggg cataaataat ctaaagtgat agcaggagta tctatggtta caggtcttca
     2521 acaaccgata gcgattccac ccggttctgg ccaacaattg aaatccctgg gtgtgacgca
     2581 caagcttacg agcgttcaaa cgaatggggc ttattatctt tgtgaagcga ttttt
//
LOCUS       DASTJP010000855         4859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1351146, whole genome shotgun sequence.
ACCESSION   DASTJP010000855 DASTJP010000000
VERSION     DASTJP010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351146"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFQ23_24840"
     CDS             <1..438
                     /locus_tag="VFQ23_24840"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909898.1"
                     /translation="SQRYGYLFRNTLFVCNKAGKPVATCSHWKAYGKINTIHWFKTLK
                     AYEGKGIGRALLSAIMRRYESTDYPIYLHTQPGSFRAIKLYSDFGFQLLRGGRLGTRM
                     NELEACLPILQEFMPKKDFEGLVIIDTPGDLITLLANEATIQF"
     gene            631..1068
                     /locus_tag="VFQ23_24845"
     CDS             631..1068
                     /locus_tag="VFQ23_24845"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET9909899.1"
                     /translation="MSEYKPSPRQWVAEQVERYEGSGGTAGFKWRDTDLPVIIVTNRG
                     RKTGAIRKTPLMRVADGNNYILVASQGGAPKHPEWYHNLKADPNVEIRDKTEVYAMRV
                     REIVDTLERDRLWKIAVQAFPNYQEYQKKTDRLIPVFLAEPVE"
     gene            complement(1150..1479)
                     /locus_tag="VFQ23_24850"
     CDS             complement(1150..1479)
                     /locus_tag="VFQ23_24850"
                     /inference="COORDINATES: protein motif:HMM:NF016147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HET9909900.1"
                     /translation="METTIKVSAKSRTSAVAGAIAGVTREHHRAVVQAIGAAAVNQAI
                     KALALAINYLKGDGIQVSFVPEMVEVSIEDQVRTAIRLVIEPVSTSAITDRVVLKPSE
                     SESVDAL"
     gene            complement(1641..>4859)
                     /locus_tag="VFQ23_24855"
     CDS             complement(1641..>4859)
                     /locus_tag="VFQ23_24855"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9909901.1"
                     /translation="RLENIQLREAVAIYDLSQTISFSLDSKLVLEKTLDAALKQTNAD
                     EGSIMLLTPSGDELRIVAVRGQGRDGLLGQRTPLTSGIAGWAARQSEALILNGEVTDD
                     RFAPLWPHPEIRSAISIPMTTAGKLIGVLNVHSLQARRPFTTGQAKALSIITGTAAAA
                     LDNQALYSALEQREKHFRALIENAPDAIAVNAPDGTVLYVSPAVEKITGIQAELGKKV
                     DPFQRIHPEDMRVSRDMLKQILQTPGIPALVQFRIQHPNGEWRWLEVVGTNLIGESSV
                     DGIVVNFSDITERKRAEERLRYQAYLLSNVNDAIIATDENFQITYWNHAAETLYGWSE
                     EEALGMYGPEITKTEFMGIDTDAMLQHIREIGVWRGEVTQVKKNGTRFPCEISSLLLR
                     SESGEVIGYVSINRDITERKQAEEALRISEERFRSYFELGLIGMAITSPSKRVLELND
                     QMCTILGYSREELLRINWTEITHPQDLEADVAQFNRVIAGEIDGYLLDKRWIRKDGQL
                     IHSSIAVKCLRRMDGSVDYFVALLQDITERKLAEEAVRTSEEQYRSLFEDSPISLWVE
                     DFSGVKRRLDQLKASGIENIPVYLREHPEFVSECAHQVKVLDVNSAALKLYHARSKDE
                     LVGRLMEILPAIPIEQFERELLMLAGDQLNFEREGTDETLTGEKIHVSLRWTVAPGYE
                     DTLARVIISTVDVTERKRAEENVQLQIQRLRALRSIDLAISSSFNLDLTLDILLSQVT
                     TQLKVDAASVLLFQPFTRKLEYAAGRGFHTASIRAASVKLGEGYAGIAVLERRMIHSS
                     NLTETNPELPPVFLLMLKEEGFVDYYCVPFIVKGEVKGVLEIFHRSLLPDDLEWLDFI
                     ETLAGQAAIAIEDATLFENLQRSNQELFQAYDATIEGWSHALDLRDKETEGHTQRVTE
                     LTVELAGTSGFTSEQLINVRWGALLHDIGKMGVPDNILLKPDKLTDKEWEIMRKHPTF
                     AYEMLSPIAYLKNSLDIPYCHHEKWDGTGYPRGLKGELIPLAARLFAVIDVWDALRSD
                     RPYRNAWSVEKTMEHIRSLSGTHFDPQAVRLFLEMIERRMET"
BASE COUNT         1187 a         1282 c         1205 g         1185 t
ORIGIN      
        1 tcgcagcgat atggatacct tttcaggaac acgctgtttg tctgcaacaa agccgggaaa
       61 ccggtggcca cttgttcgca ttggaaagcc tacggaaaga tcaatacgat ccattggttc
      121 aaaacactca aggcctatga aggcaaagga atcgggagag cgctgctctc tgcgatcatg
      181 agacgctacg aaagcacgga ctacccgatt tatctgcata cacaacccgg gagtttcagg
      241 gctataaaac tgtattccga ttttgggttt cagttgctgc gaggggggcg gcttggaacg
      301 agaatgaatg aactggaagc gtgtctgccg atcctccagg aatttatgcc gaaaaaagac
      361 tttgaaggtc tagtaataat tgacacacct ggcgatctga tcacgttgct ggcgaatgaa
      421 gcaaccatcc aattttgata cagtagaatc cacctggtca ccagacgctt tgaactcaaa
      481 gcaattcgtt ggtctcgatg cgacgaacac tcagggctac tcgaccaacg gaacttgcca
      541 caaatctaaa actgtcaggt gacgagtaga agtacagaga taaagaatac tgcctaccag
      601 tgaggcagtc ttacaggaaa ggatctgcaa atgagcgaat acaaaccaag tccacgacaa
      661 tgggtggcag agcaggttga acgttacgaa gggagtggag gcacggctgg ctttaaatgg
      721 agagatacag atttgccggt catcattgtg accaatcgcg ggcgtaaaac aggcgcaatc
      781 cgaaagaccc ccctcatgcg ggtggctgat ggaaacaatt acattctagt ggcatcgcag
      841 ggaggagcgc caaagcaccc ggagtggtat cataatttga aagccgaccc gaatgtcgaa
      901 attcgcgata agacagaagt ctacgccatg cgagtgcgag agatcgttga cacgctggaa
      961 cgagaccgac tctggaaaat tgccgtgcaa gcgtttccaa actatcagga ataccagaag
     1021 aaaacagatc ggctcatccc ggttttctta gccgaacctg tagagtgagc ctgaaggcga
     1081 ttccctcact gcagggcatt cgtatttaaa cccggctgtg cttaagaatt gttgccttcc
     1141 ccttaaaagt tatagcgcat ctactgattc agattcagaa ggcttaagga caacgcggtc
     1201 cgtgatcgct gaagtagaaa ccggctcgat caccaaccgg atcgccgtcc ggacttgatc
     1261 ttcaatgctg acttccacca tttctggcac gaaagagacc tggatgccat cccctttcag
     1321 gtaattgatt gccagcgcca gagccttgat tgcctgattc acggccgctg caccaatcgc
     1381 ttgcactacc gctctatgat gctctcgtgt aaccccggca atggcccctg ccaccgccga
     1441 agtacgtgac ttggcagata ctttgatggt tgtttccata gtatgcttcc tccaatgttt
     1501 acctgctcat ttggtcttgc gaattcagta ctatagaagt tttagtcccg atcgcctcgt
     1561 aaaattactg ctatttccat tatccacccg attctgaggc gagtcaatta gggttttcat
     1621 gtatgccatc aggatcgctg ctacgtttcc attcttcgtt ctatcatttc aagaaataga
     1681 cgaaccgctt gtgggtcaaa gtgagtacct gagagagagc gaatatgctc catcgtcttt
     1741 tcaacgctcc aggcgttacg atacgggcgg tcggaacgga gcgcgtccca cacatcgatt
     1801 actgcaaaga gccgcgcagc cagaggaatc aattcgcctt ttagcccgcg cggatagccg
     1861 gttccatccc acttttcatg atggcaatac ggaatatcca gcgagttttt tagataagcg
     1921 atgggagata acatttcgta agcaaaggtg ggatgtttcc tcatgatttc ccattctttg
     1981 tccgtcagct tatctggttt gagcaggatg ttatctggca cgcccatctt gccgatatca
     2041 tgcaatagcg ctccccagcg aacgttgatc aattgctccg atgtaaagcc agacgtgcca
     2101 gccagctcta cggtcaattc cgtgacccgc tgggtatgcc cctcggtttc cttgtcgcgc
     2161 aggtcgaggg catgagacca gccctcgatg gtggcatcat aggcctggaa gagttcctgg
     2221 ttggaacgct gcaggttctc aaataaggtg gcatcttcga tggcgatggc ggcctgccct
     2281 gcaagtgtct cgataaagtc caaccattcc aggtcatctg gaagaagact gcggtgaaaa
     2341 atttccagca cgcccttcac ctcgcccttt acaatgaaag gcacacagta atagtcaaca
     2401 aagccttcct ccttgagcat gagcaggaag acaggtggca attcagggtt ggtttccgtc
     2461 aaattggaac tgtggatcat gcggcgttca aggacagcga taccggcata gccttcgccc
     2521 agtttcacac tggctgcacg aatcgaagcg gtatgaaagc ctcggccagc tgcatattca
     2581 agtttccgtg taaagggctg gaacaacaag acagacgctg catccacctt cagttgtgtg
     2641 gtcacctggc ttaacagaat atcgagcgtc agatccaggt tgaagctgga gctaatggca
     2701 agatcgatgg agcgtaatgc cctcaggcgc tggatttgaa gctggacgtt ctcctccgcc
     2761 cgtttgcgct ccgtcacatc caccgttgaa atgatcactc tggcaagtgt atcttcgtat
     2821 ccgggagcaa ctgtccagcg cagactgaca tgaatttttt cgccggtgag cgtttcatcg
     2881 gtgccctctc gctcaaaatt aagttgatca ccagccaaca tgaggagttc acgctcaaac
     2941 tgttcaatgg ggatggccgg caaaatctcc atgagccgcc ctaccagttc atctttactt
     3001 ctggcatgat acagctttaa cgcggcgctg ttcacatcca acactttgac ctgatgtgca
     3061 cattctgaaa cgaattccgg atgctctcgc agatagacag ggatattctc aatgccactg
     3121 gccttcagtt ggtcgaggcg acgttttacc cctgagaaat cctccaccca gagtgaaatg
     3181 ggtgaatctt caaacaggct tcgatactgt tcttcgctgg tgcgtaccgc ctcctctgcc
     3241 agtttgcgct cggtgatatc ttgcagcaaa gccacgaagt aatccaccga accatccatg
     3301 cgacgcaagc atttcactgc gatgctggag tgaatcagct gaccatcctt acggatccag
     3361 cgtttgtcca gtaaatagcc gtcaatttct ccagctatca cccggttgaa ttgagcaaca
     3421 tcggcctcca gatcctgggg gtgggtaatc tctgtccaat tgatccgcaa cagttcctcc
     3481 cgtgagtagc ctaaaatcgt acacatttga tcgtttaact ctagaacacg tttggacggt
     3541 gatgtgatcg ccatgccgat cagccctaac tcgaagtaac tgcggaaacg ttcctcactt
     3601 atcctcagtg cttcctccgc ctgtttgcgc tcggtgatat cgcgattgat gctcacataa
     3661 ccgattacct cgccactttc actgcgtagc aaaagcgaag aaatctcaca ggggaagcga
     3721 gtaccatttt ttttaacctg tgttacctct ccgcgccata cgccaatctc acgaatatgc
     3781 tgtaacattg catccgtatc aattcccatg aattcagttt tggtgatctc gggaccatac
     3841 atcccaagag cctcttcttc gctccaacca taaagcgttt ctgcggcatg gttccagtag
     3901 gtgatttgaa agttttcatc ggtagcgatg atggcatcgt tcacattgga aaggagatag
     3961 gcctggtatc gaagccgttc ctcggcgcgc ttgcgctctg taatatcaga aaagtttacg
     4021 acaatgccat ccacacttga ttccccaatc agattcgtgc caaccacctc cagccaccgc
     4081 cattcgccat tcgggtgttg gatgcgaaat tgaacaagag cgggaatgcc cggagtttgc
     4141 aggatttgct ttagcatgtc gcggctcacg cgcatatcct ccggatgaat gcgctggaac
     4201 ggatccacct ttttacccaa ctcggcttgt atgcccgtga tcttctctac cgccgggctg
     4261 acgtataaga ccgttccatc cggggcgttc actgcgatgg catcgggtgc attttcgatc
     4321 agcgcccgga aatgcttctc acgctgctcc agcgcactat agagggcctg attgtcgagt
     4381 gcggcagcag ccgtgccggt aatgatactc aatgccttgg cttgtcccgt tgtgaatgga
     4441 cggcgcgcct gcaagctgtg gacatttaag acaccgatca gctttccggc cgtggtcatt
     4501 ggtatcgata ttgccgagcg aatctcgggg tgcggccaaa ggggtgcaaa gcgatcgtcg
     4561 gttacttctc cgttcaaaat cagcgcttca gactgacggg ctgcccaacc ggcaataccg
     4621 ctggtgaggg gtgtacgctg acctaacaac ccatctcgac cctggcctcg tacggcaacg
     4681 attcgcaatt catcgcccga gggcgtaagc agcatgattg acccctcgtc cgcattggtc
     4741 tgtttcagcg cggcatccag cgttttttca agcacgagtt tggagtccag tgaaaatgaa
     4801 atcgtctggc tcaagtcata aatggctacc gcctcacgca gctggatgtt ctccaggcg
//
LOCUS       DASTJP010000856        11468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_217186, whole genome shotgun sequence.
ACCESSION   DASTJP010000856 DASTJP010000000
VERSION     DASTJP010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11468
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217186"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1068)
                     /locus_tag="VFQ23_24860"
     CDS             complement(184..1068)
                     /locus_tag="VFQ23_24860"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9909902.1"
                     /translation="MKFATVSTLRATSQPALVKDDRVYTLPYPDMLAVMAAGSEKAAS
                     RASKDSFALDEVSFHSPIHPTTLRDGYAFEQHVVTANRNRGREVPEEWYQFPVFYFTN
                     PNAVFGHEDVIPYPPYTQAMDYELEIAVVIGKGGINIKPEEAPDHIFGYTIWNDWSAR
                     DIQRKEMIVGLGPAKGKDFASSFGPVIVTHESIADKATDRPGVYDLSMVARINGEEFS
                     RGNFKDIHYSFGEIIARASDSVTLYPGDVIGSGTVGTGCLLELTKFQGPWLKAGDIVE
                     LEIERIGVLRNTVGAPGK"
     gene            complement(1142..2278)
                     /gene="hppD"
                     /locus_tag="VFQ23_24865"
     CDS             complement(1142..2278)
                     /gene="hppD"
                     /locus_tag="VFQ23_24865"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HET9909903.1"
                     /translation="MATTVSENASSSRPMTEEADFLQIKSIDHLHFYVGNAKQAMYYW
                     WKAFGFKPIAYSGLETGNRDFASYVLESGQARFVVSAPYTPSSPLAAHHLLHGDGVKM
                     IALEVDDVETAWKVTTERGGKSAWAPREEKDDHGFYHTSAIYTYGETLHVFVDRSDYK
                     GVFAPTYRPIKYEAESAGLAAVDHVVGNVQLGKMDHWVNYYHQVMGFRQLLHFDDKDI
                     STEYSALMSKVMQNGNGRVKFPINEPAEGRRKSQIEEYLDYYLTPGVQHIAIITGNIL
                     QTVEKLRARGVEFLRVPDTYYEVLPDRVGTIKEDLKAIHELGILVDRDDEGYLLQIFT
                     RPMQDRPTMFIEVIQRHGSQGFGKGNFKALFESIEVEQERRGNL"
     gene            complement(2398..2853)
                     /locus_tag="VFQ23_24870"
     CDS             complement(2398..2853)
                     /locus_tag="VFQ23_24870"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HET9909904.1"
                     /translation="MFEDIRNFLPLSENLFTGGMPKSDQLTDAAKRGVEVVINLAVED
                     LSNGQSEEGELVESLGMKYINVPVVWHEPTRENLDDFMNAMDSHKDRKLLVHCEANYR
                     ATGFVALYRILRLGWDQNEAFQDVHKVWDPEKFPIWQKFIEENLPKNSE"
     gene            complement(2853..3332)
                     /locus_tag="VFQ23_24875"
     CDS             complement(2853..3332)
                     /locus_tag="VFQ23_24875"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9909905.1"
                     /translation="MSTTLSPTAQKFQELLKSLGYDYTVIEHAESTRTAQEAADRAGC
                     ELGQIVKSLIFKGKESGKPILVLTSGANRVDEKRISEYAGEAIGRADADFVRAVTGYA
                     IGGVTPIGHVQKMESYIDEDFLQYETIWSAAGTPNAIFELKTSDLQKMTNGKVVRVK"
     gene            complement(3512..3922)
                     /locus_tag="VFQ23_24880"
     CDS             complement(3512..3922)
                     /locus_tag="VFQ23_24880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909906.1"
                     /translation="MNPENQFDFWLGEWDAVWGEDGKGTNQVSRILDDKVIHEDFNGP
                     GMHGISHSVYDPERKLWCQTWVDNNGSYLDFTGTFEDEKMILMRDAIVRGESCKQRMI
                     WYNIEADEFDWNWERSDDGGETWRTLWQIKYKRK"
     gene            complement(3940..6117)
                     /locus_tag="VFQ23_24885"
     CDS             complement(3940..6117)
                     /locus_tag="VFQ23_24885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909907.1"
                     /translation="MSLKIEISVHLTLLIVLLTSCSSAPTSVASALPPTNIPSTATGN
                     GASPFESGKRYYDTSGDMEVSILDVVAFQATVNQEEEMLDVLLWVRDIPDMVNRTQPS
                     NLIEYSWTIDVYLTPADSTDLRSDYKLSMSTPVSDASTSGDVLSSTPGQPDSIPFYDL
                     FVNSNIYNSAGQTTGTVHVDITPDRNTLRFTGHVPGITTNAVFSFDMSYNEDARDQPD
                     GFVPAERSTPSPEIIFPAIDNTTQLVPIGNVHAFPGPKHYAGDILTFEIETSSSFDEA
                     FTVSMALDDGTPVDVPATFMPYGQVLMPMALDTTDLSGQHRLTFTTADGELNETYFFE
                     VLTADQRPKNEDHAVWLVQETDCCIFHYLSDTAAARDIDFISEHFQQGANEFEKIMQA
                     EIGRKVEVYIIDRILRNGAFGGSGKVLLSYTDRYYGPTVNGAGMQILARHELSHAADI
                     SLEATIDGVEFDYEGLALYVAGGHFKTEPLAQRAAALFDLGHSVPVNEIIPQHELSYL
                     HAAAILTYIEDTYDEAKVWEFLKADTTKDEQLISMKEAIQLTFGISLEEFDQGFQAWL
                     EKNDPGEQLDDLRLTIELQDLRREYQDVFASEPRFLQMEGVEAVARPENLPIVMREAH
                     APSNIAVELIIAHGQQAIVEGDYARAQELIRILGEILSTGKFEDPLAKEYLDIVLAAT
                     KEGYEVLDLDIQGDQATARLAAETPTLLNLNLQKIDGTWQIQP"
     gene            complement(6131..8923)
                     /locus_tag="VFQ23_24890"
     CDS             complement(6131..8923)
                     /locus_tag="VFQ23_24890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909908.1"
                     /translation="MKLNSPNQLMFRTYLVVLLVSILSSCGTTPTITITPPTPTPVPP
                     QPTNAEPATDTSSMFKAGLSYSDASGDMEVSFLDVVNFQATVNEETEMLEVLLHMRDI
                     PPTASLGQVKNLVEYVWSIYVYLDPPTVSPADIPGDFYFTLNTDIISPSIGSVTPIPG
                     DPSNVPINELFEHKNVYRSSGANVAELDVTADPNLDTLLLRGRVPGISSNAGFSFAMS
                     YYDGTQDRPDNIIPNDMPVFNTGLSYNDASGDMEISFLDVTKFQAAVNEELEIVEIVL
                     HMRDVPLTATRRQMRNVAEYMWRIPIFLDPSTNNPANSQADYYLLLWTLATDPPTGQA
                     DEVLTPRPGEPETVPIDQLWDDKFINNKQGDYISGVEVVADPDLDTITLKAPIPGITA
                     SSAFSFSTSSYGVETDRPDDAIASETVNLPTPLPFANQPSGSSETPIAIDETTQLIPA
                     GTVHAYPGPKHYAGDVLTFEIHNDGVFGDGTLDVEMTLDDNPPTNVSATTSFINILLP
                     QALDTTNLSGPHKVNLTTADGLLNESYSFEVLPADQRPENEIGAEWMVNEIDCCKLHY
                     ITQTAAARDIEFIAEHFQQAGEDFETITGANIDQKLDVYIMDRIWGNGGFGGNGEVVI
                     SYTDRYYGPTMGGSGLETLARHEFSHAAGIGAEPGGDGVLFNYEGLAVYVAGGHYKPE
                     PLAERGAALFDLGHYVSVTQFIPQHELSYLYSATMLTYIVETYGIDKMWEFLAADDNT
                     EDDQPGSLEGALQTTFGVSVQEFDESFQAWLESKDPGEQLQDLRLTVELQDLRREYQD
                     TYSPPAYFLLARAEDAVARPEYVPVVMREANNAPNIAIELIIAQGQQAILDGDYLRAE
                     ELIEVLRDVVTTGKFEDPLAKEYLDIVLAAASAGYEVVSVEIQGDQAIAQATAAPPEL
                     ISIELQKVNGTWQVQP"
     gene            9014..9709
                     /locus_tag="VFQ23_24895"
     CDS             9014..9709
                     /locus_tag="VFQ23_24895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9909909.1"
                     /translation="MSDQQRKIYQNDGDRYEALISREDFQGNIPRAIDEIISVDGLDI
                     FDLGAGTGRLTLMLAPRAKSIRAFDASDEMLRVCRERLVKSGLSNWQVDVADHRQLPV
                     EDGSADLVVSGWSVAYLAVWHPDSWRDELGKWLTEMKRVLNGAGTIILFESLGTGNES
                     PVQLDHLKDYYPWLDEAGFQSKWIRTDYKFESIEEAEELSRFFFGDELGDKVLKNNWI
                     VLPECTGVWWKRI"
     gene            complement(9711..11246)
                     /locus_tag="VFQ23_24900"
     CDS             complement(9711..11246)
                     /locus_tag="VFQ23_24900"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9909910.1"
                     /translation="MEMKKGSRVAVIGAGISGIAAASILKKNGIEAIVFEKYEKLGGV
                     WATAYPGIHLQNIYNQYHLSDFDWPFKPDLHPTGEQVMRYLGAAVNHLNLDIRFKHEI
                     LELKEQANGWLVRYKNESGIHEETFEYVIIAAGQYTDGKNNPQFLDQELFKGKIITER
                     EITSLDIFNGKRVVVVGYGKSALDMAVLAAERSAQVHHVFRSANWLIPEWILGAHFTY
                     ALFTRFGNVMMTSWAQPTAMERFLHNRLPFVISSFWDFIQSIVKFQLDRNGKGKDQET
                     KDRLKVLYPEHKILMDFRSSGALGPENYYPLVAEGKIFPYHSEIECFSHDSVQLKNGR
                     VIPCDVVVLSVGYLTPAFPYLPARYRTLVESEKDGPQLYRHLIHPRIPRLAFAGFNHG
                     YMHVPSVEIGTQWLSAYWRGELETPSVEEMERSIEHIRKWKRENIKFENARSSAVSTR
                     FQQYIDILLKELDVSPYRKLPNVFAEIFTRYEASDYKGIYEEYERKKAKRSAPLKTLP
                     LDT"
BASE COUNT         2825 a         2830 c         2767 g         3046 t
ORIGIN      
        1 agtacgtatc attgggtcca acatcggttg ggatcggaac accatcggtt ggtactggtg
       61 ctgcgggctt ttcattttct gcgagaaacg gtgcgttgat tggcaggatg gcaaggacga
      121 aatactgttc atccgtggtg agccctgtaa tagaacgtgc ttcacgcagc aggctgatga
      181 agattatttt cctggggcgc ctacggtgtt tcttaagact ccgatacgtt cgatttccaa
      241 ttcaacgata tctccggcct tgagccacgg gccctgaaac tttgtcagtt ccagtaagca
      301 acctgttcct acagtcccag agccaattac atcaccagga tataacgtca cagagtcaga
      361 ggcacgcgcg atgatctcgc cgaacgaata atggatatct ttgaaattac ctctggaaaa
      421 ttcttcccca ttgatccgcg ccaccatcga tagatcatac acgccagggc gatcagtggc
      481 tttatcggca atcgattcat gagtgacaat gaccggtcca aacgaagacg cgaaatcctt
      541 gcccttggcg ggacccagcc ccacgatcat ttcttttcgt tggatatctc gcgccgacca
      601 atcgttccat atcgtatagc caaagatgtg atctggtgcc tcttcaggtt tgatattgat
      661 tccacctttg ccaattacca cggcgatttc aagttcatag tccatggctt gtgtgtaagg
      721 cggatacggg ataacatctt catgtccaaa aacggcattt ggattggtga aatagaatac
      781 ggggaattga taccattctt ccggaacctc acgtccgcga ttccggttcg cagtgaccac
      841 atgttgctcg aatgcatatc cgtcgcgaag cgtggttgga tgaatgggtg aatgaaatga
      901 gacttcatca agggcaaagg agtccttgga tgcgcggctt gccgccttct cagagccagc
      961 cgccatcacc gccaacatgt cgggataggg cagggtatag actcgatcgt ccttaaccag
     1021 agcaggctgg gatgtcgcgc gaagagtgga aacagtagcg aatttcataa ttatgattca
     1081 ggtcgttgcg aaggggatcg agatgcgatt gcatcaaaat cacaacgctt tgacattcct
     1141 gttataaatt cccgcgccgt tcctgctcca cttcaatgga ctcgaacagg gctttgaaat
     1201 tgcctttgcc gaacccctgc gagccatgac gctgaataac ttcgatgaac atggtcgggc
     1261 gatcctgcat cggtcgggtg aagatttgca ggagataacc ttcatcgtca cggtcaacga
     1321 gaatcccgag ctcgtgaatg gctttcagat cttctttgat ggtacccacc cgatctggaa
     1381 ggacttcgta gtaagtatct ggcacacgca gaaactccac accgcgggcg cgcagttttt
     1441 caacggtctg aagaatattg cctgtaatga tcgcaatatg ttgcacacca ggtgtgaggt
     1501 agtaatccag atattcttca atctgactct tgcggcggcc ctccgccggt tcattgatgg
     1561 ggaacttgac gcgtccattc ccgttctgca tcaccttgga catgagtgcg gagtattcgg
     1621 tggaaatgtc tttatcatca aagtgcagta actggcggaa gcccatcacc tggtgataat
     1681 agttgaccca gtgatccatt ttgcccaatt gcacattacc aactacatga tctaccgcag
     1741 caagaccggc ggattcggct tcatatttaa tgggtctata ggtgggggca aagacacctt
     1801 tgtaatcgga ccggtctaca aagacatgca gggtttcacc gtacgtgtaa atggctgagg
     1861 tgtgataaaa gccatggtcg tctttttctt cgcgcggtgc ccaggccgat ttgccgccac
     1921 gctctgttgt gactttccag gccgtttcaa cgtcatctac ttccagcgcg atcattttta
     1981 caccatcacc atgcaaaagg tgatgagccg ccagtgggct ggatggagta tagggtgcag
     2041 aaacaacaaa ccgcgcctgc cccgattcca acacgtacga agcaaagtcg cggttgccgg
     2101 tctcaagtcc agaatacgca atgggcttga aaccgaaagc cttccaccaa tagtacattg
     2161 cttgtttagc attgcccacg tagaagtgaa ggtggtcaat ggatttgatt tgcaggaaat
     2221 ccgcctcttc tgtcatcggg cgggacgagc tcgcgttttc tgaaacagtt gtagccattt
     2281 agcctccttg ggtgaaatat taactctaaa aggattgtac gacaaagaat gcttgattgc
     2341 aatgaaaaaa tggaggagtg aatcttctta tcattttccc cttagaatcc gaattaacta
     2401 ctcagagttt tttggcaggt tttcttctat gaacttttgc cagattggaa atttctcagg
     2461 atcccacacc ttgtgcacat cctgaaatgc ctcattctga tcccatccca gccgcaagat
     2521 acgatacaac gcgacgaatc cggtagcgcg ataatttgcc tcacaatgca ccaacagctt
     2581 tcgatctttg tgagagtcca tcgcgttcat aaagtcatcc aggttttcgc gagttggttc
     2641 atgccagacg accgggacgt tgatgtattt cattcccagc gactcgacca gttcgccttc
     2701 ctctgattga ccattggaca ggtcctccac agccaggttg atcaccacct ccacgccacg
     2761 tttggctgca tccgtcagtt gatcagattt aggcatcccg ccagtgaata gattttcaga
     2821 gagaggcaaa aaattacgaa tatcttcaaa cattacttta ctctcactac ctttccattt
     2881 gtcatttttt gtagatctga ggtctttaat tcaaagattg cattcggtgt tcctgcagcc
     2941 gaccagatgg tctcatattg cagaaaatct tcatcgatat aggattccat tttttgtaca
     3001 tgtccgatag gagtcacgcc gccgatcgcg tatccggtca cggctcttac aaagtccgcg
     3061 tccgcgcggc cgatggcttc tcccgcgtac tcgctgatgc gtttctcgtc gacgcgattg
     3121 gcgccactgg tgagcaccag aatgggtttt cctgactcct ttcctttgaa gatgagtgac
     3181 ttcacaattt ggccaagctc acaacccgcc cggtcagcgg cttcctgcgc ggtgcgggtc
     3241 gattcagcat gttcgatcac ggtgtagtca tagccaagcg acttgagcaa ttcctgaaac
     3301 ttttgtgcgg tgggggagag tgtagtagac atacgataat tataaaatat tgtgtttgtc
     3361 attgcgagag cggtgacgtg gcacatctac tgttcagccg gagattgctt cgccctgtgg
     3421 tttcgatatg ccttcacctg cactgcgcgc aggcgcaagt gtcgcaaaag gcgctcaagg
     3481 ctactcaacc agccggctcg caacgaaatc cttatttccg tttgtacttg atctgccata
     3541 acgtgcgcca ggtttcaccg ccatcgtcag agcgttccca attccagtca aattcatcgg
     3601 cttcaatgtt gtaccaaatc atccgttgtt tacaggactc tcctcgaacg attgcgtcac
     3661 gcatgagaat cattttctca tcttcaaatg tgccagtgaa atcgaggtaa gagccattgt
     3721 tatctaccca ggtctggcac cacagcttac gttccgggtc atagacggag tgactgatcc
     3781 catgcatacc gggaccgttg aaatcctcat ggataacctt gtcatccagg atgcgactga
     3841 cctggtttgt tcctttgccg tcttctcccc agaccgcgtc ccattcgcca agccagaaat
     3901 cgaattgatt ctcaggattc atgtatattt ctcctcactt tatggttgga tttgccaggt
     3961 tccatctatc ttctgcaagt tcagattgag caaagtcggt gtttcagcag cgaggcgcgc
     4021 cgttgcctga tccccttgaa tatcgagatc caaaacttcg tagccttcct tcgtggcagc
     4081 taaaacgata tccaggtatt cttttgccag cggatcttca aacttaccag tagacaaaat
     4141 ctcgccaaga atccggatca actcttgtgc tcttgcataa tcgccctcga cgattgcctg
     4201 ctgtccgtga gcgatgatga gctcgacggc aatattggag ggcgcgtgtg cctcgcgcat
     4261 cacaatgggc aggttttccg ggcgcgccac cgcttctacc ccttccattt gaaggaaccg
     4321 cggttcggaa gcaaaaacgt cttgatactc gcgtcgcaag tcttgcaact caatcgtcag
     4381 cctcaaatcg tcaagctgtt cgccggggtc gttcttttcc aaccaggctt gaaatccctg
     4441 atcgaattct tcaagagaaa taccaaatgt caattggatg gcctctttca tagagatcag
     4501 ctgttcatct ttggtagtat ctgctttgag aaactcccat actttcgctt catcataggt
     4561 atcttcgatg taggtcaata tcgcggctgc atgcagatac gacaattcat gctgcgggat
     4621 aatttcattg accggcacag aatggccgag atcaaatagc gcggcggcgc gctgggctag
     4681 tggttcggtc ttaaagtgac ctcccgcgac atagagtgcc agaccttcgt aatcgaattc
     4741 cacgccatct atcgtagctt ccaggctgat atcggctgca tgagaaagtt catgccgcgc
     4801 cagtatctgc atgccagcgc catttactgt gggaccgtaa tagcgatcgg tataagataa
     4861 cagcaccttg ccgctgccac caaacgctcc gtttcttaag atgcggtcta tgatgtagac
     4921 ctccactttc cttccaattt ctgcttgcat gattttttca aactcattcg caccctgttg
     4981 gaaatgctcg gaaataaaat caatgtctcg tgccgcggcc gtatctgaaa ggtagtggaa
     5041 aatacaacag tctgtctcct ggaccagcca caccgcatgg tcttcatttt tcgggcgttg
     5101 atcggctgtc agcacctcaa agaagtaagt ctcgttcagt tcgccatccg cagtggtaaa
     5161 tgtgagccga tgctgaccgg acaggtctgt agtgtctaac gccatcggca tcagcacctg
     5221 gccgtaaggc atgaaggtcg cagggacgtc tacgggtgtt ccatcatcga gagccattga
     5281 aactgtgaat gcctcatcaa agctgctgga tgtttcgatt tcaaacgtca aaatatcacc
     5341 agcataatgc ttcggcccgg gaaatgcatg gacgttgccg atcggtacca gctgtgtagt
     5401 gttgtcaatc gctggaaaaa ttatctccgg agatggcgtc gaacgctcag cagggacaaa
     5461 cccatcaggc tggtctctgg catcttcgtt gtagctcata tcgaagctga acactgcgtt
     5521 tgtggtgatt ccgggcacgt gtcctgtaaa tctcaatgta ttccgatccg gcgtgatatc
     5581 aacatgcaca gtccccgtcg tctggcctgc cgaattgtat atattgctgt ttacaaagag
     5641 gtcgtagaag gggatggaat caggttgacc tggagtcgag gacaggacat ccccggaagt
     5701 agaagcatcg ctaacgggtg tgctcatgct tagcttgtaa tcgctgcgta aatctgtgga
     5761 gtcggctggg gtcaaataca cgtcgatggt ccaggagtac tcaatcaggt tgctgggttg
     5821 cgtgcgatta accatatctg gaatgtcccg tacccacagg aggacatcca gcatttcttc
     5881 ttcctggttg acagttgcct ggaacgccac aacatctaga atactgactt ccatatctcc
     5941 cgaagtatca tagtagcgtt ttccggattc aaacggagaa gcaccgttac ctgttgcagt
     6001 cgatgggata tttgttggcg gcagagcaga tgcaacggag gtcggcgccg acgagcagga
     6061 agttagcagg acgatcaaca aggtaaggtg gacagaaatc tcaatcttca atgacatcta
     6121 aatacctaag ctatggttga acctgccagg taccatttac tttctgcaat tcaatagaga
     6181 tcagctcggg tggcgctgca gtagcctgcg caattgcctg gtctccctga atctctacac
     6241 tcaccacctc gtagcctgcg ctcgctgcag ccaacacaat gtcaagatat tctttcgcca
     6301 acggatcttc aaatttacca gtggttacaa cgtcacgaag gacttcaatt aattcttcgg
     6361 cccttaagta atcgccatct aaaatggctt gctgtccttg ggcgatgatc agctcaatcg
     6421 ctatgtttgg tgcattattt gcttcccgca tcaccaccgg cacatattct ggacgagcca
     6481 ctgcatcttc ggctctggca agtaaaaagt aagcaggtgg tgaataagta tcttgatact
     6541 ctcgccgtaa atcctgaagc tctacggtca atcgcaaatc ttgcagttgt tcgccgggat
     6601 ctttactttc cagccaggcc tggaaacttt catcgaattc ctgcactgaa actccaaacg
     6661 tcgtttgaag cgctccctca agtgaaccgg gttgatcatc ctcggtattg tcatccgccg
     6721 caagaaattc ccacatttta tctatgccat acgtctcaac aatgtaagtc agcatggtag
     6781 ccgaatatag ataactcaac tcgtgttgcg gaataaattg agtaaccgac acatagtgtc
     6841 ccagatcaaa taaagcagcg ccgcgttctg ccagaggttc aggtttatag tgcccgcccg
     6901 caacatagac tgccagtcct tcgtaattga aaaggacacc gtctccccct ggttccgcgc
     6961 caataccagc tgcatgagaa aactcatgac gagccagcgt ctccaaacca ctgccgccca
     7021 tcgtaggacc gtagtagcga tccgtatagg agatgaccac ctcaccatta ccgccaaacc
     7081 cgccgttgcc ccagatacga tccataatat aaacatccaa tttctgatcg atgttcgcgc
     7141 cagtgatcgt ttcaaaatcc tcacccgcct gttgaaaatg ctcggcaatg aattcaatgt
     7201 cgcgcgctgc ggcggtctgt gtaatgtaat gcaatttgca gcaatctatt tcattgacca
     7261 tccattcagc gcctatttca ttttccgggc gttgatcggc tggtaatact tcgaaagaat
     7321 aggattcgtt taagagtcca tctgctgtcg taaggttgac tttatgcggc ccgctaaggt
     7381 tcgtagtgtc aagcgcctga ggcagcagaa tattgatgaa tgaggttgtc gcagaaacgt
     7441 tagtaggcgg gttgtcatca agcgtcattt ccacgtcaag tgttccatct ccgaatactc
     7501 catcattgtg gatttcaaag gtcaatacat cgccagcata atgtttagga ccgggataag
     7561 cgtgtacagt cccagcagga atcagttgtg ttgtctcgtc aattgctata ggagtttcag
     7621 aggatcctga cggttggttg gcgaaaggaa gtggagttgg aagatttact gtctctgaag
     7681 caatcgcatc atcgggtcga tcggtttcca caccgtatga agatgtggaa aaactaaagg
     7741 cagaacttgc tgtaatgcca gggatgggtg ctttcaaggt gatcgtatcc agatcagggt
     7801 cagcaacaac ctccacacct gagatgtaat cgccctgttt gttattgatg aatttatcat
     7861 cccaaagctg gtcgatcgga acagtttcag gttcgccggg ccttggagtc aggacttcgt
     7921 ccgcttgccc ggtgggtgga tctgttgcga gtgtccacaa aagcaaatag tagtctgcct
     7981 gcgagtttgc tggattattt gtagaaggat ccagaaatat tggaattctc cacatgtact
     8041 ctgcaacatt tctcatttgc cggcgtgtcg cagtcagcgg gacatcgcgc atgtggagga
     8101 cgatttcgac gatttctaat tcctcattga ccgcggcttg aaacttggtg acatccagaa
     8161 agctgatctc catatcgccg gatgcgtcgt tgtaactcag ccctgtattg aacacaggca
     8221 tatcattggg aataatattg tcgggtcgat cctgtgttcc atcatagtaa gacatggcaa
     8281 aactgaaccc cgcattagac gaaatgcctg gtacgcgccc tcttaaaaga agcgtatcca
     8341 aattcggatc ggcagttaca tctaactctg ctacgttcgc gcctgatgac cggtagacgt
     8401 ttttatgctc gaatagttcg ttaatcggaa cgttgctggg atcgcccggg ataggtgtta
     8461 cacttccaat cgagggcgat ataatatctg tgttcaaagt gaaatagaaa tctcccggaa
     8521 tatccgctgg actgaccgtt ggaggatcca aataaacata aattgaccac acatattcga
     8581 cgagattctt aacctgtccg agtgacgcgg tgggtgggat atcacgcata tgcaggagga
     8641 cctcgagcat ttcggtttct tcattaaccg tagcctggaa attgacgaca tccagaaagc
     8701 ttacttccat atcgccggac gcgtcgctat aacttagacc cgccttaaac atcgatgaag
     8761 tatcggttgc aggttccgca ttggttggct gtgggggaac cggtgtgggc gttggaggag
     8821 taatggtaat ggtcggtgtt gttccacagg aacttaatat gctaacgagc aatacgacga
     8881 ggtaagttcg gaacataagc tgattggggg aattgagttt catttgtacc tccagaaata
     8941 ggagtgtctt ataatctacc aagtcatact tggattgaca ccacccttaa gaactatctt
     9001 tggagaaacg gtcatgtctg accagcagag aaaaatctat caaaatgatg gcgatcggta
     9061 cgaagcatta atttcacggg aagattttca gggaaacatt ccgcgagcaa ttgatgagat
     9121 tatcagtgtg gatggactgg atatctttga cctcggtgcc gggaccgggc gcctgactct
     9181 catgctggcg ccgcgcgcca aatccattcg cgccttcgac gcctctgacg aaatgctcag
     9241 ggtatgccgc gaaagactgg ttaaaagcgg gttgtccaat tggcaggtgg atgtggctga
     9301 ccatcgccaa ttgcctgtgg aggatggttc tgcggatctg gtcgtctcgg gctggagtgt
     9361 ggcgtatctt gccgtgtggc atccggattc atggcgtgat gagttgggga agtggttgac
     9421 cgaaatgaaa cgcgtattaa atggagccgg gacgattatc ctgtttgaat cgctgggcac
     9481 agggaatgag tcgcctgttc agctcgatca tctcaaagat tattatccct ggctcgatga
     9541 agccggattc caaagcaaat ggatacgcac cgactataaa tttgaatcca tagaagaagc
     9601 tgaggaattg tcgcgctttt tctttggcga tgaactgggt gacaaagtcc tgaaaaataa
     9661 ctggatcgtt ttacccgaat gtacaggcgt ttggtggaag cggatttgag ttaagtatcc
     9721 agtggaagag ttttcaatgg tgctgaacgc tttgcttttt tgcgctcata ctcctcataa
     9781 atccctttgt aatcagatgc ttcgtatcgg gtaaatatct ctgcaaagac attgggcaat
     9841 ttgcggtagg gagatacgtc cagttccttc agaaggatat cgatatattg ttggaaccgc
     9901 gtactaaccg cgctggaacg tgcattttca aacttgatgt tttctcgttt ccatttgcgg
     9961 atgtgttcaa tgcttctttc catctcttcc accgacggag tttccaactc accgcgccag
    10021 taagcgctca accactgcgt tccgatttcc acacttggca catgcatata gccatgattg
    10081 aacccggcaa aagccagccg cggaatacgt gggtggatca agtgcctgta gagctgaggt
    10141 ccatcctttt cggactcaac caaagttcga tatctcgcag gtagataagg aaaggccgga
    10201 gtgagatacc caacagataa aacaaccaca tcgcatggaa tgacacgacc gttcttgagt
    10261 tgaaccgaat catgggaaaa gcattcgatc tcagagtgat acggaaagat ttttccctct
    10321 gccaccagcg gataataatt ctcaggaccc agcgcgcctg aggagcggaa atccatcagg
    10381 atcttatgtt ccggatacag gacttttagc cgatctttcg tctcctgatc ttttcctttg
    10441 ccgttccggt ccagttgaaa tttgacgatg gattgaatga aatcccaaaa gctcgaaatg
    10501 acaaatggaa gacggttgtg aaggaaacgc tccattgccg tgggttgtgc ccaactcgtc
    10561 atcatcacgt ttccaaaacg cgtaaatagt gcataggtga aatgcgcacc cagaatccat
    10621 tcagggatga gccagttggc tgaccgaaat acatgatgta cctgggcact tcgttcagcc
    10681 gccagaaccg ccatatccaa cgcgctcttg ccgtacccca ctactacaac acgctttcca
    10741 ttaaatatgt ccagcgaggt tatctcgcgt tctgtgatga tttttccttt gaatagctcc
    10801 tggtcaagga attgtggatt atttttacca tcggtatatt gaccggctgc aatgatcaca
    10861 tactcaaatg tttcctcatg tataccactc tcgtttttgt aacgaaccag ccagccgttt
    10921 gcttgttcct ttaattcaag gatctcgtgt ttaaaacgaa tatccaggtt gagatggttg
    10981 accgctgcgc ccagatatct catcacttgc tcaccggtgg ggtgcaggtc aggcttgaag
    11041 ggccaatcga aatcagatag atgatattgg ttgtaaatat tctgcagatg gattcccgga
    11101 tatgcggttg cccagactcc cccaagcttt tcgtatttct cgaaaacaat ggcttcaatt
    11161 ccgttcttct tgagaatgct tgccgcggca atgcctgaga ttccagcgcc gatcaccgca
    11221 actcgtgagc cctttttcat ctccatcggt catttctcct gtgcattcaa aaatccatca
    11281 agcctccaaa ccggaaactt gatttcatat tcaattattc acggctggat tttatttagt
    11341 ttccgatggt atggcacccc tctgaagaag tatcttagga ggatggtccc tggaactggg
    11401 atgcatcaat tcaatttgct cggcaagcca atccaccata tcgaaatacg cttgaggcgg
    11461 aagtccgt
//
LOCUS       DASTJP010000857         4856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_610774, whole genome shotgun sequence.
ACCESSION   DASTJP010000857 DASTJP010000000
VERSION     DASTJP010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610774"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFQ23_24905"
     CDS             complement(<1..839)
                     /locus_tag="VFQ23_24905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9909911.1"
                     /translation="MMQRWRKLESSLGVVLNDGAKFMFMIFATVGLLLCLGYQLLAVP
                     YRYSLDYGEAPLVDQAMRLAAGQPIYYADLSKPPYTISNYPPLYVTVLAGSVSILGPA
                     QTFPVGRILSAFSTWIASLCLGLIIYSQTRDRFAAAVAALTFVAFPFVLYWSPLLRID
                     MLALALSLSALYILISEPYSPRRLFISAVLLVAAIYTRQSYALAAPLAGFVWLLTRDW
                     RQALRLAAMVGGLTLALFFVLNGLTGGGFYFNIVTANVNEFKLDLLRFNWERLRDAAL
                     LPV"
     gene            complement(1118..4846)
                     /locus_tag="VFQ23_24910"
     CDS             complement(1118..4846)
                     /locus_tag="VFQ23_24910"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9909912.1"
                     /translation="MNCPNCGFNSPSSMRFCGLCGTRLTINCPECSSANPLDYRFCGM
                     CGTRLNGEAEPLVHAPSIFLPKTEDEPLLPAETSPLEGERRVVTVIVTDLTESTNLLE
                     NVGTEGWVDLMNRILHILESEINRFGGEVSQFRGDGLVAFFGATSAHEDDPERAVLAS
                     LSMQRTFDLFVRELAQPDAAELRMRVGINTGEVIVPSGSDRQHAQETAMGMAVAIAAR
                     METAAEAGTVLVSEHTYHLIESQFDWDALGEIFVKGVSQPIAVYRPRRHVTDAELPSQ
                     GNAFPDAMPLIGHEDEFQMLKSCVQSLFEGRGGIATLTGEKGYGKSFLLNEVRHYFAH
                     RRALLSESEPTSSSANISVTWVRSRCRSYSQTWPYSMWLELFRDWLGLRPDASKEEKR
                     ATLYRRAQEIWGAALEEHYPYLATFLGLPLEDNFTEKIRHLDGEGLRQRFFIAVRSWI
                     EGMSRNGPLVLSFSDMQWVDDSSLSLLKYCLPICDNESLLWLMSFRPERETSIWEFNH
                     YLEVEYPHRLTSVELLPLTPAQSLKLINHLIGSETLPEETSNLILRNAEGNPYYILEL
                     IQALIAKDVLEREPENGPWRLTRPVTTLDLPNSLQRLLLARIDRLTAQQRLILQIAAV
                     IGSVFWQNMLNHLLGDSPRLKTDLAALQRAQLIQESGRVPELGMQYFFKSPLVRETVY
                     DSLLSAQRITFHLRAAEYLENIISQNVLSEYDGMLAYHYRGAGNHRKELFYTFLAAEN
                     ERKIYANAEALQDYTRVLELLGILERETPSNGLSRSIQTQRFEALNGRRQVYFRLGKF
                     EEARADTQALLPLARQMADDPVWLVDALLAQSQIARDDRKELVPGLHMAEEALSLAKQ
                     SGEKRRIMQSLDAVADIRFTLNDPGWREFADHAVSLAKEFGDLRTEVKLLLAISGKYG
                     SDDLPRGREYLQEALARSETLNDKATKLAVLNAIGLQFERDGDYYQQLTKYEQERLRL
                     SREIGNRIVEGNALMFCGQIQALYLGDYEKGLQLQIQTLNFWENITDRLFPLLRIAQI
                     QTEMEHHEEALAALELARPLQEKVVLDIGRAGFGLVSAILYNSLGDEASLRSSLSVTS
                     QIQQMVSNSLVSRQYHMVAACEASLAHLKLAQLLTKKEDGNAESQYHLPKALEYSQTA
                     LQMYEEFGFVQVVECTSEEVLFRHSCALTANGRTEEGTEFLKRAHKEMMRKYELIPTE
                     SPYRKTYLENIKLHRDIQAAHKKQTSSTAKRRKTKQRA"
BASE COUNT         1192 a         1266 c         1234 g         1164 t
ORIGIN      
        1 atgacgggca gcaatgctgc gtcacgtaag cgttcccagt taaacctgag caggtctaac
       61 ttgaactcat tgacattagc ggtcacaatg ttgaagtaga agccgccgcc tgtcaatccg
      121 ttgagaacaa aaaacaaagc caatgtgagt ccgcctacca tcgctgcgag acgcaacgcc
      181 tgccgccaat cgcgcgtcag gagccagacg aatccggcca gtggagcagc gagcgcatag
      241 gattgccgcg tgtagatggc agctacgagt aacaccgcag aaataaacaa tcgtcgcgga
      301 gaataaggct ctgaaatcaa tatatatagg gcggataagc tgagagccag cgccagcata
      361 tcgatccgca aaagtggcga ccagtacagc acaaacggaa acgctacaaa ggttagggct
      421 gctaccgccg ccgcgaatcg atcacgcgtt tgagagtaaa tgatcagccc caagcaaagg
      481 ctggcaatcc aggttgagaa ggctgataaa atgcgcccga ccgggaacgt ttgggcgggc
      541 ccaaggatgc tcacgctgcc tgctagaacc gtcacataga gaggtgggta atttgaaatc
      601 gtatagggcg gtttggataa gtccgcgtaa taaataggtt gcccggcggc caggcgcatt
      661 gcctgatcga ctaacggtgc ctcaccataa tctaaagaat agcgatacgg gaccgcgaga
      721 agttgataac ccaaacaaag aagaagcccc actgtcgcga aaatcatgaa catgaacttg
      781 gcaccatcat tcagcactac cccaagtgat gattccagtt ttctccatcg ttgcatcatt
      841 ggattttacc ttagtgggga ggtagaacgt taagtgcggg agtggcgaat gtagtgtgtc
      901 agccacacac ctgcctgatg tgctcgagca aatcacaggc ggcgctaggg gagcccacag
      961 agttttttct caaagggctc tgtgctctct gtggcgaagt agattatcac tcactctctt
     1021 agccactccg ttgatgcccc gctctcgaaa acaattgtga agcgcacagt cttgatggaa
     1081 ggagaaacaa gccgatgttg atgcagagac cgccagctta ggcgcgttgc tttgttttgc
     1141 gtcgttttgc ggttgaagag gtttgttttt tatgagcggc ttgtatgtca cgatgtagct
     1201 taatattctc cagataagtt ttccgatagg ggctttcggt aggaatcagc tcatatttac
     1261 gcatcatttc cttgtgtgcg cgcttcagaa actcagttcc ttcctccgtg cgaccgtttg
     1321 ccgtcagggc gcagctatga cggaagagaa cctcttcgct ggtacactcc acgacctgga
     1381 caaacccaaa ttcttcgtac atctgcaacg cagtttggga atactccaac gcctttggca
     1441 gatgatattg gctttccgca ttgccatctt cttttttcgt aagcaattgc gccagcttca
     1501 aatgtgctaa cgaagcctcg caagccgcaa ccatatgata ttgcctggaa acaagactgt
     1561 tagacaccat ttgttgaatc tgtgaagtta cgctcagcga agaacgaagt gaagcttcgt
     1621 cgcccagcga gttgtaaaga atcgcgctga cgagtccaaa tccggctcgg ccgatgtcca
     1681 aaacaacttt ctcctgcaag gggcgagcca actccaaagc ggcaagagct tcttcgtgat
     1741 gttccatttc ggtttgaatt tgcgcgatgc gcaacagtgg gaacaggcgg tctgtaatgt
     1801 tttcccaaaa attgagagtt tgtatttgta attgcaatcc tttttcataa tcccccaaat
     1861 aaagcgcctg aatctgacca cagaacatga gcgcgttgcc ctcaacaatt cgattgccga
     1921 tctctcggct cagacggagt cgttcctgtt cgtattttgt tagctgttga taataatcac
     1981 catcacgctc aaactgtaga ccaatcgcgt ttagaactgc caactttgtg gctttgtcgt
     2041 tcaacgtttc tgagcgcgcg agtgcctcct gaaggtattc tcgtccgcga ggcaagtcat
     2101 ctgacccata ttttccactg atggccagca ggagtttcac ttctgtgcga aggtctccga
     2161 actctttcgc aagcgatacg gcgtgatcgg cgaattcccg ccaaccgggg tcgttcagcg
     2221 tgaatcgaat gtcggcaaca gcgtccaggc tttgcatgat gcgccgtttc tcaccagatt
     2281 gcttcgcaag ggataacgcc tcttccgcca tgtgcagccc tggaaccaat tcctttcgat
     2341 catctctggc gatctgcgat tgtgcaagca atgcatcgac caaccagacc ggatcatccg
     2401 ccatctggcg cgccagcggg agaagcgcct gggtgtcggc gcgtgcttct tcaaatttac
     2461 ctagccgaaa ataaacctga cggcgaccgt ttaatgcctc gaagcgttgt gtttgaatag
     2521 aacgacttag tccgttcgat ggagtttcac gttccagtat gcccagcaat tccaggaccc
     2581 tggtgtaatc ctggagtgct tccgcgttcg cataaatctt tcgttcgttt tcggcagcaa
     2641 gaaaagtgta gaacagttcc ttgcgatggt tgcccgcgcc gcggtaatga taggcaagca
     2701 ttccatcata ttcactgagc acattctggc tgattatgtt ttcgaggtat tctgcggctc
     2761 gaagatgaaa tgtgatccgt tgcgcgctta agaggctgtc atagacggtt tcccgcacaa
     2821 gcggtgattt gaaaaagtat tgcatcccca attcaggaac acgaccactt tcctgaatga
     2881 gctgggcgcg ctgaagggct gcaaggtcgg ttttcaatcg cggggaatcc cctaacaaat
     2941 gatttagcat gttctgccaa aatacagatc cgatgactgc cgcgatctga agaatcaacc
     3001 gttgctgagc tgtcaaccga tcgatgcggg caagcaacag gcgttgaagg ctgttgggca
     3061 ggtcgagtgt tgtcacaggg cgtgtcagac gccagggacc attttcaggc tcacgctcga
     3121 gaacatcttt tgcgatgagc gcttgaatga gctccaaaat gtaatagggg ttaccttcgg
     3181 catttcgaag gatcaagttg ctggtttctt ctggaagcgt ctctgagccg atcaagtggt
     3241 taattaactt caggctttgc gcaggggtga gtggcagtaa ttccacgctc gtcaatcgat
     3301 gaggatattc aacctcgaga tagtggttga actcccagat ggaagtttcg cgctccggac
     3361 ggaatgacat cagccacaac aaactttcgt tgtcgcagat tggcaaacaa tatttcaaga
     3421 gactcaatga tgagtcatcc acccattgca tgtctgagaa agagagcacc agcggaccat
     3481 ttcgactcat cccttcgatc caacttcgaa cggcaatgaa aaatcgctga cgcaggcctt
     3541 ctccgtccaa atgcctgatc ttctctgtga agttatcttc gagcggcaac cccagaaatg
     3601 tcgcgagata gggatagtgt tcttcaagag cggcacccca aatttcctgc gcgcggcgat
     3661 acaaggtggc acgtttttcc tctttggatg catccgggcg cagcccgagc caatcgcgga
     3721 ataactctaa ccacatggaa tacggccagg tctggctgta cgaccgacag cgactacgga
     3781 cccaggttac tgaaatgttc gccgaagacg aagtaggttc tgattcactc agcagtgctc
     3841 gacgatgagc aaaataatgg cgaacttcat tcaacaggaa ggattttccg tacccttttt
     3901 cgcccgtcaa cgtggcaata ccaccccggc cttcaaaaag gctttgcacg caactcttaa
     3961 gcatttggaa ttcatcttca tgcccaatca gcggcatcgc atctggaaat gcgttcccct
     4021 gtgatgggag ttccgcatcc gtgacgtgtc gtctgggtcg atacaccgcg atcggctgac
     4081 taaccccttt cacaaaaatc tcgcctaagg catcccagtc aaattgcgat tcgatcagat
     4141 ggtaggtgtg ttcgctcacg agaacggttc cggcttctgc ggcagtctcc atacgagcgg
     4201 caattgctac cgccatcccc atagcggtct cctgcgcgtg ctgacgatca ctgccgctgg
     4261 gcacaatgac ctctccagtg tttatgccca ctcgcattcg gagttcggct gcatcaggct
     4321 gtgcaagttc acgcacgaag agatcaaacg tgcgttgcat cgataacgaa gctaatacgg
     4381 ctcgctccgg gtcatcctca tgggcagagg ttgcgccaaa gaaagctacc agcccatcgc
     4441 cgcggaactg gctgacttca cctccaaagc gatttatctc tgattccaaa atgtgcagaa
     4501 tgcggttcat caggtccacc cagccttctg ttccgacgtt ctccagtaga ttcgtagatt
     4561 cggtcaggtc cgttacgatg accgtgacca cacggcgttc cccttccagg ggtgacgtct
     4621 ctgcgggcag cagtggctca tcttctgtct ttggcaggaa gatggatggc gcgtgaacga
     4681 gcggttctgc ctctccattc aaccgcgtgc cgcacatccc gcagaaacga taatcaagtg
     4741 ggttagcaga actgcactcg gggcagttga tcgtcagccg cgttccgcat agtccacaga
     4801 agcgcattga cgaagggctg ttaaaaccac aattaggaca gttcatattc cattaa
//
LOCUS       DASTJP010000858         4729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_356050, whole genome shotgun sequence.
ACCESSION   DASTJP010000858 DASTJP010000000
VERSION     DASTJP010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356050"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFQ23_24915"
     CDS             <1..628
                     /locus_tag="VFQ23_24915"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9909913.1"
                     /translation="SVRSMFENIILAYGGVDNVIITAGIFVPPDKLGYIPDDKWSLTY
                     AINVTGPYLVADEAKKIWQAQGLRTSLVITTSANAAVAKQGSFAYDTSKAAANHLVRE
                     LAIELSPLVRVNGLAPATVVEGSSMFPRDRVISSLTKYNISFDEGESTESLRNKLAAY
                     YAQRTLTKSPITLTDQAEVAYLLSSSKFSKTTGQIISVDGGLHEAFLR"
     gene            731..1105
                     /locus_tag="VFQ23_24920"
     CDS             731..1105
                     /locus_tag="VFQ23_24920"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9909914.1"
                     /translation="MPKTKPTTVDEYIKAAPDVAQAKLKEIRAILKKVAPKAKEGLKW
                     GNPVFEDGRILFSYSAYKSHLNFSPTGPAMKPFKEELSKFKTGKDTIQFAYDKPLPKT
                     LIRKIAAFRAKQVKENDARWMY"
     gene            1251..2234
                     /locus_tag="VFQ23_24925"
     CDS             1251..2234
                     /locus_tag="VFQ23_24925"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9909915.1"
                     /translation="MLKLKYLFDNPELAHMLLKNWDYDESSVDMFHSYRISANAVYPF
                     TRNEKVHLLRFCPASEKIKDNLIAELEFIAYLRNNQYPALEAVPSKTGEELVQKMTPW
                     GEYFASVFKRVNGTQLSDMPLENDLLFAYGSALGQLHKLSSKYTALVAERWTHVEVLA
                     WIKQILRNISTEDAALQELEYLRVKFSQLPVNAENYGLIHYDFELDNIFYDPQAKSCN
                     VIDFDDAMYHWYVMDIEQALDSLKGEVTESEFEQKKAYFLDGYKTQFAIDDELFESRL
                     LFRRFANLLGYARVTRAIQEKWENEPDWLIQLRVKLNESLRRRSAYFGVQA"
     gene            2521..3480
                     /locus_tag="VFQ23_24930"
     CDS             2521..3480
                     /locus_tag="VFQ23_24930"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET9909916.1"
                     /translation="MKRCSILVLFVSLILTACQPEAIGWRQLQLSTNPTASGGGAISY
                     NSDAHFALYLPGNLAETWIWENGSWSQSHPADQPPPRAKFTMAYDKINNQIVVFGGIY
                     GETLYDDTWVWDGKNWIKMETPHTPPARCCSAMAYDESLGQILLYGGWDSRTNTFLSD
                     LWIWDGTDWTELTCCSMPLMSGHKMVTYPPEILSTFTAGLGTQIWDGKNWQALEITSP
                     PDRPDSALVYDSHQDLVVLFGGKRDALLLNDTWIYDGEDWMELRFPIVPPVRDAHAMF
                     YDERRGSIILFGGVGETGVLGDIWELNLAEDLSGFIVLPTAQP"
     gene            3863..4192
                     /locus_tag="VFQ23_24935"
     CDS             3863..4192
                     /locus_tag="VFQ23_24935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909917.1"
                     /translation="MIEYQDWETTVRYMPEVEEETWNSLLQVESQHVPISPDLDIGYH
                     YVLVEDVPSSTCTYRFSEVGFNENQDQALVVLTVQYDDFYSGSLYLFKLVDGQWKAVR
                     ELLLWIT"
     gene            4324..>4729
                     /locus_tag="VFQ23_24940"
     CDS             4324..>4729
                     /locus_tag="VFQ23_24940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909918.1"
                     /translation="MVKRHWLKWVLVSYLTLMVVVLTARFVLPTSHWLSWGIDHFLLI
                     FFVPIPILFLICIHLRESFILLALPAFAFLVLYGRLFSPARQPEHRSDAIPIRVMTFN
                     VLFTNLDQEAVAQVIKDSGADLVALQELTPRNG"
BASE COUNT         1279 a         1040 c         1146 g         1264 t
ORIGIN      
        1 cagcgtgcgg tcgatgtttg aaaatattat tctggcttat ggcggtgtgg ataacgtcat
       61 tattaccgcg ggaatttttg tcccgcctga caaactgggc tatatcccgg acgacaaatg
      121 gtcactgacc tatgccatta acgtcacggg tccttacctt gtggcagatg aagcgaaaaa
      181 aatctggcag gcccaagggt tgaggacaag ccttgtgatc acgaccagcg caaatgccgc
      241 cgtggccaag cagggctctt ttgcttatga caccagcaaa gcggcagcga atcatttagt
      301 acgcgaactt gcaattgaac tctcgccgtt ggtgcgcgtc aatgggttgg cacccgcaac
      361 cgttgtggaa gggagcagta tgtttccccg cgaccgcgtg atcagttcgc ttacgaaata
      421 caacatctcg ttcgatgaag gcgaaagtac cgaatcgtta cgaaacaaac ttgcggcata
      481 ctatgcccaa cggacgctga ctaaatctcc catcaccctg accgatcaag ccgaagttgc
      541 gtatttgcta agcagtagta agttcagcaa gacgacaggg cagattatca gcgtggatgg
      601 gggattgcat gaggccttct tgcgatagct aaactagtat gcacctcgtt aggcaacaag
      661 cctaacagaa ccatacccgt atggcatgaa gaacgggctc gttaataacg atgaatacgg
      721 aggttcacac atgccaaaga ccaaaccaac cactgttgat gaatacatca aagcggctcc
      781 tgatgtggcg caagcgaaat tgaaagaaat acgcgccatc ctgaaaaagg tcgcgccgaa
      841 ggccaaagag gggcttaagt ggggaaaccc agtatttgaa gacggtcgaa ttctattctc
      901 gtattctgca tataaatcac atctcaactt ctcgcccaca ggacctgcga tgaaaccgtt
      961 caaagaagag ttgtcaaagt tcaaaactgg caaggatacg attcagtttg cttatgacaa
     1021 accgcttccc aagacgctta ttcgaaagat tgccgcgttt cgtgcgaagc aggtgaagga
     1081 aaacgatgcg cgctggatgt actagtgcat ctgatcccaa ctttattttt aggatgatta
     1141 gtagaacaaa acgtagctat gagtagcctc gcggcagtct taattgcatc gccagagccg
     1201 aatagcaata gcaataatgt agggttaatc agtctggtgt gacactcatc atgctcaaat
     1261 tgaaatacct cttcgacaat cctgagctag cgcacatgct gctgaaaaac tgggactacg
     1321 atgaatcttc cgtagatatg ttccactcct atagaatttc agccaatgcg gtctatccat
     1381 ttacaaggaa tgaaaaagta cacctgttgc ggttttgtcc agcctcagag aaaataaaag
     1441 ataatctcat tgctgaactt gagttcattg cctatttgcg gaacaatcaa taccccgctt
     1501 tagaagctgt gccctccaaa acgggtgaag agcttgtgca aaaaatgact ccgtggggtg
     1561 agtatttcgc gagtgtcttc aaacgtgtca atggaacaca gctaagtgac atgccactgg
     1621 aaaatgacct cctgttcgcc tatggatcag ctctggggca gctgcataaa ctatccagca
     1681 aatatacggc cttggtggca gagcgctgga cccatgttga ggtgcttgcc tggatcaagc
     1741 aaatacttag aaacatttca accgaagatg cagctttaca ggaattggag tatctcagag
     1801 taaagttctc tcaattacca gttaacgcgg agaattatgg tctcatacac tatgactttg
     1861 aactcgacaa tattttttat gaccctcagg caaaatcctg caatgtgatt gattttgacg
     1921 atgcgatgta ccattggtac gtcatggata tcgaacaagc gctcgacagc ctcaagggcg
     1981 aggtaacgga atctgaattc gaacaaaaga aggcgtactt tctcgatggg tacaaaacac
     2041 agttcgcgat tgacgatgag ttgtttgaat cccgactgtt gtttcggcgc tttgccaatt
     2101 tgctcggcta tgcccgtgtt acgagagcga tccaggaaaa gtgggaaaat gaaccagact
     2161 ggttgatcca attgagggta aagttgaatg agtcactccg aagaagatca gcctactttg
     2221 gagtgcaagc atagcccagt cgcaaatgcc agcttcatag gtggacaatt aggttctcag
     2281 caggtgccag agagcacact ggcacgagtg ctataatatt tttagcgaaa ttaatcccgc
     2341 tgtaaaggat tatggttgac cgtatgaagt cccaatcgct ttgggtttta agcggactgc
     2401 ccattagacg gacgcaagcc ccgcctgttg gtggagcgca gtgtccgtct aaaatctctt
     2461 atacggcagc cgtatggccc tggttggcgc tcgatgtttc aaaggaggta aggaagctat
     2521 atgaaaagat gttcaatcct cgttttattc gtaagcttaa tcttaactgc atgtcagccc
     2581 gaagcgatag gatggagaca acttcagctt tcaaccaatc cgaccgctag cggaggcggc
     2641 gcgatttcct acaacagtga tgctcatttc gctctttatc ttcccggcaa tctcgctgaa
     2701 acctggattt gggagaatgg aagttggtca cagtcccatc ctgcagatca accccctcca
     2761 cgtgcaaagt tcacaatggc atacgataag atcaacaacc aaatcgtagt gtttggcggc
     2821 atttatggcg aaacgcttta cgatgatacc tgggtatggg atggcaagaa ttggatcaag
     2881 atggaaactc ctcatacgcc tcccgctcga tgttgcagtg ctatggcata tgacgaatcc
     2941 ttaggccaaa ttcttttata tggtgggtgg gacagtcgta ccaacacatt tctaagtgat
     3001 ttatggatct gggacggcac agattggact gaattgacat gttgctctat gccattaatg
     3061 tccgggcata agatggtcac ttatccaccc gaaatcctat ccactttcac agctggttta
     3121 ggaacacaga tttgggatgg aaaaaattgg caggcactag agattacaag tccgcctgac
     3181 cgaccggata gcgcgcttgt atatgatagt catcaggatt tggtggttct atttggcggc
     3241 aaacgagatg ccttgctcct aaatgataca tggatttatg atggggaaga ttggatggaa
     3301 ttgagatttc caattgttcc tcctgtacgt gatgcccatg ctatgttcta tgatgagaga
     3361 cgaggctcta tcattttgtt tggtggcgtc ggtgaaaccg gagtgctagg tgatatatgg
     3421 gagctgaatc tggcagaaga tttatctgga ttcattgtgt tgccaaccgc tcagccatga
     3481 ggaaataccg aagagcgccc aacaatgcgt gcaggcgaac ttggcggtac gcacagcgct
     3541 agaaccgtcg tctcgacgag gagctttttt ttccgtttcg tgagttggtt ctcccaccaa
     3601 ggcgctaacg cgggctgttg agcagttttc attgaccgat tcaatttcta taacaagtta
     3661 cagcatgaaa ttaacaagaa aaactgtact gattctcaca attacaattc ttttgttatc
     3721 ctgttcccct gttcagaaaa gcgaagaaga gcgcctgtcc gaaatatatt ctcaggcaat
     3781 aagaatgtat aggcaaggtg atccgatccg attctgctcg gaagataatt tgagcattat
     3841 ttctgtaact gattctttta ggatgataga ataccaagac tgggaaacga ccgttcgtta
     3901 catgcctgag gtggaagaag aaacgtggaa tagtcttctt caggttgaat ctcaacatgt
     3961 gcctatctct ccagatttgg atattggtta tcactatgta ttggtagagg atgtgccaag
     4021 ttcaacctgc acatataggt tttcggaggt agggttcaat gaaaaccagg atcaggcact
     4081 ggttgttttg actgtgcaat atgacgattt ttattctggt agtttgtatt tgttcaagtt
     4141 ggtggatggt caatggaaag ccgtaaggga attattatta tggattacct gatttcttat
     4201 ggcagtgtct tatatcttac caaatagaga gagttgcgcc aaatactgcc caacactgtg
     4261 tgcagcaact gtctggggag acttgcgctg tataagtagt tatcgtatta ctggatcctt
     4321 cgcatggtaa aaaggcattg gctgaaatgg gtattggtca gctacctgac tctcatggtc
     4381 gtggttttga ctgcccggtt cgtccttcca acaagccatt ggctcagttg gggaatcgac
     4441 cattttctac ttatcttttt tgtccctatt cccatattat tcctgatttg catccattta
     4501 cgagaatcct tcatcctcct ggcattacca gctttcgctt tcctggttct ctatggcagg
     4561 ttgtttagcc ctgcccgaca gcctgaacat cgatcagatg ctattccaat ccgcgtgatg
     4621 acgttcaacg ttctctttac taatttagat caggaggcgg ttgctcaagt catcaaggat
     4681 tctggtgccg atttggtggc ccttcaggaa ctaaccccgc gcaatggaa
//
LOCUS       DASTJP010000859         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_505114, whole genome shotgun sequence.
ACCESSION   DASTJP010000859 DASTJP010000000
VERSION     DASTJP010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505114"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..753)
                     /locus_tag="VFQ23_24945"
     CDS             complement(172..753)
                     /locus_tag="VFQ23_24945"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9909919.1"
                     /translation="MIRAGFSFEHPTTKTRTVVLESDAETSGMGWLLEVTRYSKLGSD
                     LGEHLHLTWTETFEIIKGTAKYKLDGNERTAKAGESFVVEPGHFHVHPWNANEDELVY
                     RQRDRFAEPSPAAVQDILGIFATRTGLARDGIRYKGFAKLLFQSATIRTAIKHGNYVE
                     SPSMSMQKVLASTLGLLGELLGYKAVQPKYVGE"
     gene            complement(762..1625)
                     /locus_tag="VFQ23_24950"
     CDS             complement(762..1625)
                     /locus_tag="VFQ23_24950"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9909920.1"
                     /translation="MRLSTTNLLLASDAGIDLQLHTTYSDGVWTPEQLIDHLVEEKFS
                     LAAITDHDRLDTVAALQQLAVEKHVPLLVAAEMSASWQGGITDLLCFGFEVGPSALRE
                     LSQTVLQKQQENTREVYENLLRKGYTFQGHPEALSAILEKPGSQQPKELVALLKTTGE
                     ESAGKIVWEAGCTFMTNDLPEIVEAAHQSGAVCLLAHPGRKDGFITYDVELLDKLRQE
                     IPIDGLEVYYPAHTPEQTTMFLEYARRHNLLISAGSDSHSPEKPPIKYPAELSRSLLE
                     RLGIQILQSSQ"
     gene            complement(1622..2296)
                     /locus_tag="VFQ23_24955"
     CDS             complement(1622..2296)
                     /locus_tag="VFQ23_24955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909921.1"
                     /translation="MTNFFSVDRQGLAFLKIQWEDLSSLYASDRIIKDNLFQMLKEKY
                     SEKSRFYHNLSHIKTLLSLFESLHNKIQEHNAIRFAIWFHDVIYDSKRSDNEEESARL
                     VVEVLHKLQVNIETIEIVKEMILATKNHNGKNLTHDVKLFLDMDLAILGTSEEIYKEY
                     SKAIREEYSWASDAIYRSGRERVLRSFIERERIYLTHEMEVRYAEQARKNIDNEIKSL
                     QENISQ"
     gene            complement(2346..2894)
                     /locus_tag="VFQ23_24960"
     CDS             complement(2346..2894)
                     /locus_tag="VFQ23_24960"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909922.1"
                     /translation="MKLTVHALTPELLPALEDLFDTQGPVSRCWCMYWRIGDSYRSRP
                     SKENKAAFRKLVKKGPPPGLLAFDGDIAVGWCQLTPRDALPWLDRTWRLKRVDDLPVW
                     SISCFYTRKGYRKQGVTSVLIAAAIETAMRAGAPALEAYPFADESPSASSTGRVSIFK
                     RAGFKIVARHAPPRPILRYEFK"
     gene            complement(2896..>3439)
                     /locus_tag="VFQ23_24965"
     CDS             complement(2896..>3439)
                     /locus_tag="VFQ23_24965"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9909923.1"
                     /translation="SLFDYFHEDYAGDNKFDYYNAELLRACDFLLLSRNSFLGNKEYW
                     SDVLNNPNFTPIRRETAQLFKSIEKIVISDKLTSEELAPWENTRIISRAEAYKEIAAL
                     KQQPGRDILVLLSRLLWNDLLVHDLVDELHITYFPLIAGEGSSIFVGRPPVSLKLIQT
                     RSWHDSGNLLAVYKVDRPKS"
BASE COUNT          822 a          845 c          813 g          959 t
ORIGIN      
        1 acggtcgcgc cgagtgctct cttataccac tcaacagcag cgggcgtatc cgcaaccgcc
       61 agcatggcag aaattatcgg tctttgcata acagtctcct ttatcgaatg cgtgcttcca
      121 gggaagcata actttttcac tgaatgtgaa atgtatatta tttttgattt tctactcccc
      181 gacatatttg ggctggaccg ccttataccc cagcaattca ccgagcaacc ccagcgtgga
      241 cgccagtact ttctgcatcg acatggacgg actctctaca taattgccat gcttgatcgc
      301 agtccggatt gtggcagatt gaaacagcag tttggcaaat cccttgtagc ggatgccatc
      361 acgtgccagg cctgtgcgtg tcgcgaagat acctaagata tcctgcactg cagctggact
      421 cggttctgca aatcgatctc tttgacgata gaccagctca tcctcatttg cattccatgg
      481 gtgcacatga aagtgaccgg gttccacaac gaaagactca ccagccttcg ctgttctctc
      541 atttccatca agtttgtatt tggcagtgcc tttgatgatt tcaaaagtct ccgtccaggt
      601 gaggtgcaga tgttctccca aatcggagcc gagcttggag tatcgagtga cttccagaag
      661 ccagcccatg ccgcttgttt cggcgtcgct ttccagaacg actgtgcgcg ttttggtggt
      721 gggatgttcg aagctaaacc cggctcgtat catggcgctc ctcattgtga tgattgcagg
      781 atctgaatcc caaggcgctc gagaagagat cggctcaact cggccggata cttgatcggt
      841 ggtttctcag ggctatgtga gtctgagcct gcactgatca ataagttgtg tctgcgtgca
      901 tattccagaa acatggttgt ctgctcgggg gtgtgagccg ggtaatagac ttccaggccg
      961 tcgattggaa tttcctgtcg caatttatcc aggagttcca catcgtacgt aataaatcca
     1021 tccttgcgcc cgggatgcgc taaaaggcac acggctccgc tttggtgtgc ggcttccaca
     1081 atttcaggga ggtcgtttgt cataaaggta cagcctgctt cccagacaat tttcccggca
     1141 gactcttcgc ctgtcgtttt caggagagca actaattctt tgggttgctg tgaaccaggt
     1201 ttctcaagga tcgctgataa cgcttcggga tgcccctgaa atgtataacc ttttcggagc
     1261 agattttcat agacttcgcg agtgttttcc tgttgtttct gtaatacggt ttgagacaat
     1321 tcacgcagag cactcggacc tacttcaaac ccaaagcaaa ggagatcggt tatccctccc
     1381 tgccacgaag cgctcatttc tgctgcgacc agcaggggca catgtttctc cactgcaagc
     1441 tgttgcagtg ccgcgaccgt atccaaacgg tcatgatcgg tgatggctgc caggctaaac
     1501 ttttcttcta ctaaatgatc gattaactgc tcaggagtcc agactccatc actgtaggtt
     1561 gtatgaagct gaagatcaat cccagcatcg gaagccaaaa ggagattcgt cgttgataat
     1621 ctcattgaga tatgttttcc tgtaatgact taatttcgtt gtcgatgttc tttcttgctt
     1681 gttctgcata tcttacttcc atttcgtggg taagataaat cctttcccgc tcgataaaac
     1741 ttctgagaac cctctctctg ccgctacgat atatcgcgtc agacgcccag gaatattctt
     1801 ctcgtatcgc tttgctgtat tctttatata tctcttcact tgttccaagg atggctagat
     1861 ccatatccag aaatagtttc acatcatgag ttaggttttt accattatga ttctttgttg
     1921 ccagtatcat ctcttttaca atttctatcg tttcgatgtt cacctgtaat ttatgcaaca
     1981 cttcaacaac caacctcgca ctttcctcct cattatcact ccgttttgag tcataaatga
     2041 catcatgaaa ccaaattgca aaccttatgg cattgtgctc ctgtatttta ttgtggagag
     2101 attcaaacaa actcaacagt gttttgatat ggcttaagtt gtgatagaag cgactctttt
     2161 ctgagtattt ctcctttaac atttgaaaca aattgtcttt gatgattcta tctgatgcgt
     2221 aaagcgatga taaatcctcc cattgaatct ttagaaaggc aagaccctgt ctatcaacac
     2281 taaaaaagtt ggtcatttaa tcgaaacaaa gtgttagaag tttggagtca tagtcctcgg
     2341 tcctgttatt tgaactcata acgcaggatc ggacgaggcg gtgcatgacg cgcaacgatt
     2401 ttgaacccgg cacgcttgaa gattgaaaca cgacccgtgc tcgaagcgct gggtgactca
     2461 tctgcaaagg gatatgcttc gagggccgga gcccccgccc gcattgcggt ctctatcgcg
     2521 gctgcaatca ataccgaggt aacgccttgt ttgcgatagc ccttgcgggt gtaaaagcac
     2581 gaaattgacc agaccggtaa atcatccacg cgtttgagcc gccaagtgcg atccagccag
     2641 ggcaatgcat cgcgtggcgt aagctggcac cagccaactg ctatgtctcc gtcgaaggcg
     2701 agcagaccgg gaggtggacc cttctttact aatttacgga atgcagcttt gttctctttt
     2761 gaggggcgac tgcgatagct atcgccgatt cgccagtaca tgcaccaaca gcgactgacc
     2821 ggaccttgtg tgtcgaaaag atcctcaagc gcgggcagca gttctggtgt gagcgcatgg
     2881 acggtaagtt tcatcttacg atttggggcg atccacctta tagactgcaa gcaggttacc
     2941 cgagtcgtgc caggaacgag tctggatcag tttcagtgat actggcgggc gtccgacgaa
     3001 gatggaggag ccttctccgg caatgagcgg aaagtaggtg atgtgcagtt cgtccaccag
     3061 gtcatggacc agcaggtcat tccaaagcaa acgactcagg agtacgagaa tatccctgcc
     3121 cggctgttgt ttgagtgctg cgatctcttt ataggcctct gctcgtgaga tgatccgtgt
     3181 attttcccag ggtgccaact cctcagaggt cagcttgtcc gaaattacga ttttctcaat
     3241 ggatttaaaa agctgggcag tctcccgtcg gattggcgtg aagttgggat tattcagcac
     3301 atcggaccaa tactctttgt tgcctaaaaa cgaattccga ctcaacaaaa ggaaatcgca
     3361 ggcacgcaag agttctgcgt tgtagtagtc aaatttattg tcacccgcat agtcttcgtg
     3421 aaagtaatcg aacaatgag
//
LOCUS       DASTJP010000860         3450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_716035, whole genome shotgun sequence.
ACCESSION   DASTJP010000860 DASTJP010000000
VERSION     DASTJP010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716035"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /gene="rocF"
                     /locus_tag="VFQ23_24970"
     CDS             <1..719
                     /gene="rocF"
                     /locus_tag="VFQ23_24970"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HET9909924.1"
                     /translation="KLKYIDCIIPMGRRIAGAVATSIQGGRFPLVLGGDHSLSVGSIR
                     GAAKHRKLGVIWIDAHADFNTHETTPSGNIHGMPLAALCGLGVPSLVSLWDETPPVVD
                     PKRVAIIGARDLDPGEKDNLRSAGVMVQSMEQIDRLGMPAALEKAIERVSNDSDGIYI
                     SFDMDALDPRYAPGVGTPVPGGLTYREAHLVGEVVAETGKLVGMDLVEVNPILDVQNQ
                     TAILAVEFIRSALGSRVWSG"
     gene            917..1540
                     /locus_tag="VFQ23_24975"
     CDS             917..1540
                     /locus_tag="VFQ23_24975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9909925.1"
                     /translation="MSTLYGPILIVEDVPNVLELLEVTLRFKGYAVITARNGEEALEV
                     IARQRPVLIITDILMPKMDGYAFVQKLRLNPETRSIPVVFLSATYVTPEDKDFALSLG
                     AARFMEKPIDTEDFLLTIAELVTQQPFTQPHPLDMERFYNGYRTRLENKLRHKNTQIA
                     RAERLLPTLPHDQKPAFEALLHQSMRDRDEIQTELYDIYKTLEELKK"
     gene            1712..2518
                     /locus_tag="VFQ23_24980"
     CDS             1712..2518
                     /locus_tag="VFQ23_24980"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9909926.1"
                     /translation="MQPTLSYIENLAREAGSILLAGYEKEHQVNFKGVIDLVTEVDHE
                     SEAFLLGRIRKDFPDHHIFSEESGVIQGNDEHVWYIDPIDGTVNYAHHIPMFCVSIAY
                     AVRPKDSSSASLSANLNLGAVYDPMRDEMFTAERGAGAYLNGRALQVSSTNDLQQSLL
                     ATGFPYDAWNTPQDNFTNFIKFGKLSRGVRRLGSAALDLAYVGAGRFDGFWEMALNPW
                     DVAAGGLICEEAGARVTNVEGVDDYISPPQSVLATTPGIYARMLEVLHET"
     gene            2701..>3450
                     /locus_tag="VFQ23_24985"
     CDS             2701..>3450
                     /locus_tag="VFQ23_24985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909927.1"
                     /translation="MSTKSIPISKTKIIVPHRRPEILSRPRLLESIRALLDNKLILLS
                     APAGYGKTSLLIDLAHNIGMPVCWLSLDLLDRDPQRFMAYLIAALAEQFPDVGEAARL
                     QLNRLKSIEEDTESLLVTLTNDLYDNVENDFLLIIEDYHLLDDVQVISTLLNRFLELV
                     VENCHLILSSRTLPDLADVTLMVAREQVAGLSHTELAFIPREVQALYAQNHHQHLSDE
                     TAREFVDQTSGWITGMVLSNMPDMPRVSGIDT"
BASE COUNT          845 a          840 c          875 g          890 t
ORIGIN      
        1 caaagctcaa atatatcgat tgcatcatcc cgatggggcg gcgcattgcg ggggcagtgg
       61 caacctctat tcaaggaggg cgttttccgc ttgtgcttgg aggcgaccat agcttgtccg
      121 tagggtccat tcgcggcgcg gctaaacatc gaaaactcgg tgtgatctgg atcgacgccc
      181 acgctgattt caatacccac gaaacgactc catctgggaa tatccatggg atgcctctcg
      241 ccgcattgtg cggtctcggt gtcccaagcc tggtctcgtt atgggatgaa acgcctccag
      301 tcgtagaccc gaagcgggtc gccattatcg gagcgcgtga tctcgacccg ggtgaaaaag
      361 ataacctgcg ttcagcaggt gtaatggtac aaagtatgga gcaaatcgac cgtctgggca
      421 tgcctgccgc acttgaaaag gcaattgaac gtgtcagcaa tgactcggat gggatttaca
      481 tttcttttga tatggacgcg cttgacccac gctacgcgcc gggagtgggt accccggtcc
      541 ctggtgggtt gacttatcgc gaggcgcatc tggtgggtga ggtcgttgca gagacaggta
      601 agctggtcgg catggatctg gtcgaagtga atcccattct tgatgtgcag aaccagaccg
      661 caatcctggc ggtggaattc atccgctctg cgttggggtc acgcgtctgg agcggatagc
      721 atttgcatgt cacaagaaaa tgacgatttt ggggttttgc tggaaagcag ctatagattg
      781 ctgcccaatt aaatagattt gaaatctaaa ctttagctta cgagcgaagc acgccgcgca
      841 gcggcagtgt ggacaaaaca attgagaaag ttactaacgc atagaccatg cgttagacaa
      901 gcggtaaaat gatactatga gtaccttata tggtcccatc ctgattgtcg aagatgtacc
      961 caatgttctc gaattgctcg aagtcacgtt gcgttttaag ggctacgccg tgatcaccgc
     1021 gcggaatggg gaggaagctc tggaagtcat tgctcgtcag cgaccagtgc tgatcattac
     1081 ggatattcta atgccgaaga tggatgggta tgcctttgtg cagaaacttc gcctcaaccc
     1141 tgagacgcgg tccattcccg tggtattcct ctccgctacc tatgtcactc cggaagataa
     1201 ggatttcgca ttaagtctcg gtgcggcgcg tttcatggaa aaaccgatcg atacggaaga
     1261 ttttttgttg accatcgccg agttggtgac acaacaaccc ttcacacagc cgcatccgct
     1321 cgatatggaa cgcttctata acggctatcg cacgcggctc gaaaataagt tgcgccacaa
     1381 aaacacacaa attgcgcgtg cagaacgctt attgcccact ctaccgcatg atcaaaagcc
     1441 cgctttcgaa gccctgttac atcagtcgat gcgtgatcgc gatgagattc aaactgaact
     1501 ttatgatatt tacaaaactc tggaagaact aaagaagtag ttttattaat ttttcacgcc
     1561 tcgttgggtt caacccagcg ccgcgcattt actgcttgtc tcacgcatgg tctatgcgtt
     1621 actaaacctg ttgcaatgaa gagtatggta aaactaaaac atgtggttta gatacgcata
     1681 tgatgcgtgc ggcactccgt aagaaggccc catgcaacct acactctctt atattgaaaa
     1741 tctggctcgt gaagcgggct ccatccttct ggcaggttat gaaaaagagc atcaggtgaa
     1801 ttttaaaggc gtaattgacc ttgtcaccga agtggaccat gaatcggaag catttttact
     1861 tggcaggatt cgaaaagact tcccggatca tcatatcttt tcagaagaaa gtggcgttat
     1921 tcaggggaat gacgaacatg tttggtatat tgacccgatc gatgggaccg tgaattatgc
     1981 gcatcacatt ccaatgtttt gtgtttccat cgcctatgca gtgcggccga aggattcctc
     2041 ctccgcttcg ctcagcgcca atctgaacct gggggctgtt tacgacccca tgcgtgatga
     2101 aatgtttacc gccgaacgcg gcgccggcgc ctatcttaat ggtcgtgcgt tacaggtgtc
     2161 ttcaaccaat gatcttcagc aaagcctgct ggctaccggc tttccttatg atgcctggaa
     2221 tactcctcag gacaatttca caaacttcat aaaatttggc aagctttcac gcggtgtgcg
     2281 ccgactcggt tccgccgccc tggacctggc ctatgtaggc gcgggtcgct tcgatgggtt
     2341 ttgggaaatg gcacttaatc cgtgggatgt ggcagcaggt gggctgattt gtgaagaagc
     2401 aggcgcccga gtgacaaatg tagaaggcgt ggacgactac atatcgccac cccaatcagt
     2461 cctggcgaca acaccgggta tttatgcacg catgctggag gtcttgcatg aaacataagg
     2521 aaacttactc atcgttcctg catatactgt acagaatatc tgtcggggat ctgtcgtgtt
     2581 agattaacag ccttaccttc cccgtcgcaa tgtaagaaaa gtttgtcaca aaattgtgat
     2641 gaaattcaga tgaaaaatcc tccggaacca cataaaataa gggcagtttc atctgatcga
     2701 atgtctacaa aatctatccc tatcagtaag accaagatca tcgttccgca tcgtcgcccc
     2761 gagatactct cccgacctcg attgctggag agtattaggg cgttgctgga taacaagctg
     2821 atcctgcttt ctgccccggc gggctatggc aagacctcgc ttttgatcga tctggctcat
     2881 aatatcggta tgccggtttg ctggctttcg ctggatctgc ttgaccgcga ccctcagcgc
     2941 tttatggcct atctgattgc cgcgctcgcg gaacagtttc ctgatgtggg cgaagccgcg
     3001 cgactccaat taaatcgatt gaagtcgatt gaggaggaca cggaatcgct gctggtaacg
     3061 ctgacaaatg acctttatga caatgttgaa aatgactttc tcctgatcat cgaagattat
     3121 catctcttgg atgatgttca agttatttct acacttctaa atcgttttct tgagctagtg
     3181 gtggaaaatt gtcatctgat cctttcttca cgtaccctcc ctgatctggc ggatgtgact
     3241 ttgatggttg cacgcgaaca ggtggctggg ctaagccata cagaattggc attcattccg
     3301 cgtgaggtac aggctttata cgcgcagaat caccatcaac atctttccga cgagacagcc
     3361 cgagaatttg ttgaccaaac aagcggctgg attactggca tggtgctttc caacatgcca
     3421 gatatgccgc gcgtttccgg tattgatacc
//
LOCUS       DASTJP010000861         5078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_63283, whole genome shotgun sequence.
ACCESSION   DASTJP010000861 DASTJP010000000
VERSION     DASTJP010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63283"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFQ23_24990"
     CDS             complement(<1..290)
                     /locus_tag="VFQ23_24990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909928.1"
                     /translation="MRIAKLAFEAAGYQLVYSRKKEQPGYYLDGQSALSSEIKQVLKG
                     SAAEVDQRQIDIYRKLSFAERFHQGCSISDIARKVVAYRIHQENPRLSLAEA"
     gene            1310..1744
                     /locus_tag="VFQ23_24995"
     CDS             1310..1744
                     /locus_tag="VFQ23_24995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909929.1"
                     /translation="MKKISLLTKGTIVALLLALVLSSFPTTDVFAKGDRQNLEKKWDQ
                     LVTNYNRQSTVHNSMHKWVDHWLKAEKKASLADKVEVRKHLSICNSAISAAGSVVAIH
                     AGFDASGEVIDAGLARNSIKDLANYLRQHAGSIKNLKEHIGD"
     gene            complement(2053..2355)
                     /locus_tag="VFQ23_25000"
     CDS             complement(2053..2355)
                     /locus_tag="VFQ23_25000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909930.1"
                     /translation="MMKPVLVQIVGAQVACTDGLKDAWREVSSWAARQLSAHFGNRVD
                     VKYYDVFDADCPSMPIEAQLPLVLVNGEVTINGGKISITAIRRKVDSILEKETVNG"
     gene            complement(2352..4547)
                     /gene="arsD"
                     /locus_tag="VFQ23_25005"
     CDS             complement(2352..4547)
                     /gene="arsD"
                     /locus_tag="VFQ23_25005"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HET9909931.1"
                     /translation="MSNNILQLYTLTDVEFFDPPMCCPTGLCGPTTDQTLLDVSQTIR
                     ALQQDNYRVERYQMASNPNAFLGNAEVMQHVRQKQLQALPIIVVRGKVIKVGAYPSEK
                     EIRGALDGTGDKMTQFIFFSGKGGVGKTSMACAHAVRYADEGRRTLIVTTDPASNLAD
                     VFEQEIGHHVTPISGVPNLSAMEIDPDEATREYIDRAMSPIRGAFPPQLVQVMEEQMS
                     GPCTAEVAAFDRFTDFLDVPAENGNVFEIIIFDTAPTGHTIRLLELPDEWSQSINAAS
                     VGSGQTCIGPAAAIQDAKHKYERALSAMRQPEQTSFVFVLHPEAISINETHRAINELK
                     KLDIENYRLIINGIIPPEGVQNPLFAARAEMQSRYLDQIEREFVFPKQRMILLANEIK
                     GAQRLRQVGKIFFDGEPAPQVDETVKTQSHDEMLASPSDLVRARLQPNGQRRTIFFAG
                     KGGVGKTVTSCITAIWLAQQGHKTLLLTTDPAAHLGDVLDTPIGDEVAPVGEQPNLWA
                     VKIDPKVSAEKYRTRILNDARQRGRPESAIKMMAEELNSPCTEEMAAFDKFVEYASEN
                     DWEAVVFDTAPTGHTLRLLKLPFAWNKQIDVKAFTSVDVTAADDTAKQRFGRVIEMMS
                     DPEQSTFAFVLYPEATPILEAWRASQELSTVGIHPGLVVANMVIPPEQATTPFVQSRR
                     FMQKKYLQEITRRFNVPMVQIPLLPGEIKGLKMLTKLGEQMYGIERVAA"
     gene            complement(4641..>5078)
                     /locus_tag="VFQ23_25010"
     CDS             complement(4641..>5078)
                     /locus_tag="VFQ23_25010"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET9909932.1"
                     /translation="VLATLPYAFGVTTVIFGKHIDKIEGDRAKKIHTLPVLIGEKPAR
                     YLVMVMMILPYFLLIYLVAIKYYSPLILTVVFALPQLKVVLPVFLVPKPTRRPEDFPD
                     GEGGWPLYFASMAFWYNRSFGTLFVMSLIGEVLVRIFLPEFWR"
BASE COUNT         1314 a         1241 c         1184 g         1339 t
ORIGIN      
        1 tttgcctcag cgagtgacaa tcgtgggttt tcctgatgaa tgcggtaagc cacgaccttg
       61 cgagcgatat cactgatgga acaaccctgg tgaaagcgtt ctgcaaagga taatttgcgg
      121 taaatgtcaa tttgacgttg atccacttca gccgcgctgc ccttcaatac ttgtttgatc
      181 tcagacgata acgcggattg cccatccagg taatagccgg gttgctcctt ttttcggctg
      241 tataccagtt gataacccgc agcttcaaat gcaagtttcg cgatacgcat atcccggtag
      301 aacgtgtctc cccacgccga aaccccaaag cagtttttcc caagacggag ttcgactctg
      361 gcaatgagct gcgaccgtgt gagaaaacgt tcattccaga gaatctccag taaagcgaca
      421 cgtcgggaag tggtgatatc tcactgcact gcatcgttga gaatggcatc gttcattaca
      481 atgggtaaaa ttgactttct catctacgaa ctattatgcc tgattcatac atcagcttta
      541 tttcgagagt gtccaacatc aacaacacaa tgtcattctc ccgaagggac accgcttcgg
      601 gacgctgtga gcgacggaca ggagcgaaga atcccgccgc gcagcggcgg agacccttcg
      661 ctatgctcag ggtgacaaaa cgctttatcc tcgaagttgg acactccgtt gtttcgtttc
      721 taacttttat taagaggatt gacaacacat tgtgttgata tgatccattc tttggtcgcg
      781 aaccctaatc ccgtcgggac cgccaaatag acaacttgat gagatttcat caagttgttt
      841 ttgttttggt ctgtcagcag ggatggtagt tcggaacagg tttgcctaag ggtgtttaga
      901 attgctgaag ccagctagac ttctgagcag gagattctaa ctgggctttg tttagatagc
      961 ggggaatgcc aaatccagtc tcaatgaata agagcacatc gcaactagct aagaatccat
     1021 tcgttcagcc agtccaaaac atgtagcgca attttcccat tgagccactt tgcgaaccgg
     1081 tatatgacac ccgatgagag caaggttgcc cactgactct ttggtagata agaaaaataa
     1141 aacctaatga catgacggac agggcgatta gcctattgga taaagcaaat ctttataata
     1201 aaaatcgtct cataccaaaa tcaaacatct atttaatgta atctcgtttg gttcgtgcta
     1261 ttctacagat gtcgtttgtt ccaaatttca caaaaagaag gtgagcaaaa tgaaaaagat
     1321 ttcgttactt acaaaaggca cgattgtagc cttactgctg gcgctcgtgc tgtcttcgtt
     1381 ccccacaacc gatgtgtttg ccaaaggcga tcggcagaac ttagaaaaga agtgggatca
     1441 acttgtcacc aattacaacc gacagagcac cgttcataac agtatgcata agtgggttga
     1501 ccattggctc aaggcagaaa agaaggcttc cctcgcagac aaagtagagg taagaaagca
     1561 tctttcgatc tgcaattctg ccatatccgc tgcaggatca gtagtggcca ttcatgctgg
     1621 ctttgacgca tccggcgaag ttatcgatgc agggctggcg cgaaattcca tcaaggactt
     1681 agccaactac ctgcggcaac acgctggctc cataaagaat ctaaaggagc atatcggcga
     1741 ctaagaatcg cctgtaggca aaaaacggca gggacttcga taaagtccct gccgttgtcg
     1801 tttttgcaat ttcctattca agcgaaatat cgttgtcaac cttgagtcaa gaggctgctt
     1861 gctaattgta ggattacgtg ccacagagtc acagttccat actggctggt tcatcgaatt
     1921 actcttgacg gaggtcgtga tggccctcct ttcatacatt tctttgacac tcggcctgat
     1981 cccccttggg gtgtccgttc gacagtaaca tgcggagatg tttcaaactg taaccaaagg
     2041 attctactct gtctagccgt ttacagtttc cttctcaaga attgagtcga cttttcgacg
     2101 aatagcagtg atggaaattt tcccaccatt gatggtaact tcgccattca ctaacacgag
     2161 tggcaattgc gcctcaattg gcatagacgg gcaatcggca tcgaacacat cataatattt
     2221 cacatctact ctattaccga aatgcgcaga taattgtcga gctgcccagc tggatacttc
     2281 ccgccatgca tccttcagtc catctgtaca tgcaacctgt gcgccaacga tttgaacaag
     2341 gactggcttc atcatgcggc tactctttcg atgccataca tttgttctcc caatttcgtt
     2401 agcattttca agcctttgat ctcgccgggc aacaagggaa tttgcaccat cggcacgttg
     2461 aatcgccggg taatttcctg caaatacttt ttctgcatga aacggcggga ttgtacaaat
     2521 ggcgtcgtgg cttgctcagg cggtatgacc atatttgcaa ctaccaatcc gggatgaata
     2581 cccactgtgc tgagttcctg cgaggcgcgc caggcttcaa gaataggagt tgcctcagga
     2641 tacagtacaa aggcaaacgt gctctgctca gggtcagaca tcatctcgat aactcttccg
     2701 aagcgttgtt tggcggtatc atctgcggca gtcacatcca ccgaagtaaa agcttttaca
     2761 tcaatttgct tattccaagc aaagggcaat tttaacaacc gtaacgtgtg gccagttggc
     2821 gcagtatcga acacaactgc ctcccagtca ttttctgatg catattcaac aaatttatca
     2881 aatgccgcca tctcttccgt acacggcgag ttgagttctt cagccatcat tttgatcgca
     2941 gattccggac gcccccgctg acgcgcgtca ttcagtatac gtgtcctgta cttttctgct
     3001 gaaactttcg gatcaatctt gactgcccac aaattcggct gttcacccac aggggcgacc
     3061 tcatcgccaa tcggcgtgtc cagcacatca cctaaatgcg cggcggggtc agttgtcaac
     3121 aatagagttt tatgtccttg ttgtgccaac cagatcgcgg taatgcacga ggtgacagtc
     3181 tttccaaccc cacctttgcc ggcgaagaaa attgtccgcc tttgaccatt cggttgaagg
     3241 cgcgcgcgta caagatcaga gggcgaagcg agcatttcat cgtgcgattg ggtcttgacg
     3301 gtttcatcca cctgtggagc tggttcacca tcgaagaaaa tttttcccac ctgacgtagt
     3361 ctttgggctc ctttgatttc gtttgccagc aggatcatgc gttgttttgg gaagacaaat
     3421 tctcgctcga tttgatccag ataacgcgac tgcatttccg cgcgtgcagc gaagagcgga
     3481 ttttggacac cttcaggcgg gatgatccca ttgataatca gacgatagtt ttcgatatcc
     3541 agttttttca gttcgtttat tgcgcggtgt gtttcattta ttgaaattgc ctcaggatgc
     3601 agtacgaaca caaaactcgt ctgctcaggt tgccgcatag cggacagggc acgctcgtac
     3661 ttatgcttcg catcctgaat agcggccgcc ggaccaatgc aggtttgtcc gcttcccacg
     3721 ctggcggcgt tgatggactg gctccattcg tcaggaagtt caagtaagcg aatggtgtga
     3781 cccgttggag cagtatcaaa aataatgatt tcgaaaacgt tgccattttc ggctggaaca
     3841 tccagaaaat cagtgaagcg gtcgaaggcg gctacttctg ctgtacacgg accgctcatt
     3901 tgttcctcca ttacctgaac aagttgcgga gggaaggcac cgcggatggg tgacatggcc
     3961 cgatcaatat attcccgtgt ggcttcgtca ggatcaattt ccatcgcgga taagtttggc
     4021 acaccgctaa tcggcgtaac atgatgtccg atttcctgct caaaaacatc ggcaaggttg
     4081 gaagcgggat cggtcgtaac aattagcgtg cgcctgcctt cgtctgcata acgaacagca
     4141 tgagcgcaag ccatgctcgt cttgccgaca ccgcctttgc cagagaagaa aatgaattgg
     4201 gtcattttgt cgcccgttcc atcaagtgct ccgcgaattt ccttctcact cggatacgca
     4261 cctaccttga ttaccttacc ccgtacaacg atgatcggca aagcttgcag ctgtttctgg
     4321 cgcacgtgtt gcattacttc ggcgttgcct aaaaacgcat tggggttgct ggccatctga
     4381 tatcgttcta cacggtagtt atcctgctgg agggctcgga tcgtttgact cacatccaaa
     4441 agcgtttgat ccgtggtggg accacatagt cctgtcgggc aacacatggg cggatcgaaa
     4501 aattccacgt cggtcagggt atacagctga agaatattat tgctcatgat ggttcttcca
     4561 ggtaggttgt cagggttgtt tcagatttca ttcatcaaca caggcgctgt gagcacaaat
     4621 aaaagtctcc ttcaggattc ttagcgccag aattcgggta agaaaattcg aacaagaacc
     4681 tccccaatca ggctcatgac aaacaaagtg ccaaatgaac ggttatacca gaatgccata
     4741 gacgcaaagt aaaggggcca cccgccttcg ccatcaggga aatcttcggg acgccgtgtc
     4801 ggttttggga cgaggaaaac gggtaaaact accttcagct gcggaagcgc gaatacaaca
     4861 gtcaagatca ggggactata gtatttgatt gcaactaaat aaattagtag gaaatatggc
     4921 aaaatcatca ttaccattac caaatagcgg gctggtttct caccgattaa cactggcaat
     4981 gtatgaattt ttttcgcccg atccccttct attttgtcaa tatgcttacc aaagatcact
     5041 gttgttactc cgaaagcata aggtaacgtt gctaaaac
//
LOCUS       DASTJP010000862         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_611385, whole genome shotgun sequence.
ACCESSION   DASTJP010000862 DASTJP010000000
VERSION     DASTJP010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611385"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFQ23_25015"
     CDS             <1..404
                     /locus_tag="VFQ23_25015"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9909933.1"
                     /translation="QLDPEATAMAAFPSRDREPFMAHWSKILANGSGIQKTILFNDRV
                     AGNIMCWEQDGKREVGYWLGKEYWGKGIATQALKEFFHQMKIRPVVAHVAKHNIASRR
                     VLEKCGFTLIQEDKYYNPAGEEVEEFVLQLD"
     gene            473..1990
                     /locus_tag="VFQ23_25020"
     CDS             473..1990
                     /locus_tag="VFQ23_25020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9909934.1"
                     /translation="MNDSDSRISFLLQRIALIIFAFLPVGLSIYYQSRSLLDSLDTFS
                     FFRIGLITGLLLLPLIAILIRDDLWASIERADYAPLVWIVALGVALRLILVPLLSTNF
                     TSDFEDIHNFAVDVVSGRPFANLANYPSIPWATHLNMTGLTLSLVYRIIGASFTAAKM
                     FMVVMSGLTIWLIYLAGRDLVNARTGFVAASLYGTLPSLIGYTGIPSGENIALPLITL
                     ALLLYIRGQKTDGYKLSRSVLIYTLCGITIGLVDWFRPGGIILIAALVISDVLYTRRR
                     EWFPYRLATLCGLVISYLIVSALSVVISESFFQRPVLSTSQKIGYFVLIGLNAAEEGR
                     INLEDRDIAFQAYARFEDDNSAANNYLLQLARQRLEGASLVNLFRSKFIQAWSNHQQM
                     FDMALNGSNDQELMEVLGAIEGLVYLIITVFVGVNVYASFLKRSHPAVFAMQLFILGS
                     ALGLLILEVQNRYTIITFPYMSLLASIGMSEAASMIFGVPQAQQTSEISSRQIDA"
     gene            1998..>2366
                     /locus_tag="VFQ23_25025"
     CDS             1998..>2366
                     /locus_tag="VFQ23_25025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909935.1"
                     /translation="MKIFEREFRGNTDLASMAELVRRFPTENLHVVDLPYRFSSWSFE
                     YPENIRLWTDEGGQILAWAVLQVPFWTIDYAYHPEFHNPLHLQILKWADEQAHRAVDT
                     PSGHPAWYVSVPIQQAERIYD"
BASE COUNT          546 a          546 c          600 g          674 t
ORIGIN      
        1 aacagctcga cccggaagcc acagcgatgg ccgctttccc ttccagagac agggaacctt
       61 tcatggcgca ctggtccaag attttggcaa atggatctgg gattcagaaa accatccttt
      121 tcaatgaccg ggtagctggc aatattatgt gctgggaaca ggatgggaaa cgcgaagtgg
      181 ggtattggct cgggaaggaa tactggggaa aagggattgc gacacaagct ttaaaagagt
      241 tttttcatca gatgaagata cgtcccgtgg tggcgcatgt ggcgaagcac aatattgcat
      301 cacggcgcgt gctcgagaaa tgcgggttta ccttgatcca ggaagataaa tattacaatc
      361 cggccgggga agaagtagag gaatttgttc tacagttgga ttgatggtca accccgcgta
      421 gtaaaattaa ataaccgtgt atgtacaaac taccacggta tttccattcc atatgaacga
      481 ttctgactcc aggatttcat ttttgttgca gagaattgct ctgatcattt ttgcgtttct
      541 gcccgttggg ttatcgattt actatcaatc ccgttctctg ctggattcat tggatacctt
      601 ttcatttttc cgtatcggac tcattaccgg acttttgctg ctgcctctca ttgccattct
      661 catcagagat gatttgtggg catccatcga acgtgctgac tacgcgccgc ttgtatggat
      721 cgtggccttg ggcgttgcgc tacggctgat cctcgtcccc ttgctgagca cgaacttcac
      781 atcggatttt gaagatatac acaattttgc cgttgatgtg gtttccggca gacctttcgc
      841 caaccttgcc aactatccga gcatcccgtg ggcgacccat ctaaatatga ccgggctgac
      901 attatcgcta gtctatagga tcattggagc aagctttacg gcggcaaaga tgtttatggt
      961 ggtcatgtcc ggattgacaa tctggttgat ctatctggca ggtcgggacc tggtcaacgc
     1021 gcgcaccggg tttgtggcgg ccagcctgta tggaacgctt ccctcgttga tcggctacac
     1081 gggaatccca tccggggaga atatcgcctt gccgctcatt actctggcgc tcctgctcta
     1141 tattcgcggg cagaaaactg acggttataa actctcccgc tctgtactta tctataccct
     1201 gtgtggaatt acgattggcc tggtagattg gttccgcccg gggggaatca tactaattgc
     1261 cgccctggtc atttcagacg tactttatac ccgccgaagg gaatggtttc cttatcggct
     1321 ggcaacgtta tgtggattgg tgatcagcta tttgatcgtg agcgccctgt ccgttgtcat
     1381 ctctgagagc ttttttcagc gaccggtttt atcgacctcc caaaaaattg gctactttgt
     1441 tctgatcggg ttaaacgcgg cggaggaagg ccggatcaat ctcgaagacc gggatattgc
     1501 ttttcaggcg tatgcacgct tcgaagatga taattctgcg gctaacaatt atttattaca
     1561 actcgcaagg caaagattag agggcgcgtc gctggtgaat ttgtttcggt cgaaatttat
     1621 tcaggcgtgg tcaaaccacc agcagatgtt tgatatggcc ttgaacggct ccaatgatca
     1681 ggaactcatg gaagtgttgg gtgccatcga gggactggtc tacttaatca ttaccgtttt
     1741 cgtgggtgtg aatgtatatg catcatttct taaacggtct caccccgcgg tctttgcgat
     1801 gcaattgttt atcctgggtt ctgcgttagg attattaatt ttggaagtcc agaatcgcta
     1861 tacgattatc acctttcctt acatgagctt gcttgcttcc attgggatga gcgaggcggc
     1921 atcgatgatc tttggagttc cacaggctca acagacatct gaaatttcgt ctcgtcagat
     1981 tgatgcctga agctgatatg aaaatcttcg aacgcgaatt ccgcggcaat actgatttgg
     2041 catccatggc tgagctggtt cgaaggttcc ccaccgagaa tttacatgta gtggatttgc
     2101 cctatcgttt tagttcctgg tcgtttgagt atcctgaaaa cataaggcta tggactgacg
     2161 agggtggaca aatactggcg tgggcagtat tacaagtccc gttctggacg attgattacg
     2221 cataccaccc tgaatttcac aaccctttac acttgcaaat tttgaaatgg gcagatgagc
     2281 aggctcaccg agccgttgat actcccagcg gacaccctgc ctggtatgta tctgtgccca
     2341 tacagcaggc ggagcgaatt tatgat
//
LOCUS       DASTJP010000863         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_483822, whole genome shotgun sequence.
ACCESSION   DASTJP010000863 DASTJP010000000
VERSION     DASTJP010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483822"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFQ23_25030"
     CDS             <1..377
                     /locus_tag="VFQ23_25030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9909936.1"
                     /translation="GYQEKKSFKSWLYTITINTARSHLRKHRFAERLRQTLTSIFLVE
                     SQKSPSPEDVTIQNEQEAQLWKALNEMDEKHRMVTVLRYFHELSITEIAEILSIHEGT
                     VNSRLHTTRERLRNTLEHLDKK"
     gene            384..1040
                     /locus_tag="VFQ23_25035"
     CDS             384..1040
                     /locus_tag="VFQ23_25035"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9909937.1"
                     /translation="MQITHAEAHKLIQFNLDRTLDPEKQTSLFAHLESCASCRGYADT
                     LRNVENQLRQVMNKQWNQPPLPLSIDLLKTQLKPPRGTQNLFQLRTALVSMVLVAFSF
                     FIWQLASPSSNPSIQSPSTIFPVPTPSTQLSTVSMEPTKCEWMLYEVKATDTLDTIAF
                     KYSVSKQDIMEFNNMGSEIVNETMELKIPQCSATPTVTAQLPTTTLTPKLELFIDTPG
                     "
     gene            1156..>2417
                     /locus_tag="VFQ23_25040"
     CDS             1156..>2417
                     /locus_tag="VFQ23_25040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HET9909938.1"
                     /translation="MEKKSTTWLERPVHPALPAITNEIAIFAGIILLAIITRFYDLGT
                     RVMSHDESLHTYFSWLLYRGQGYQHTPMMHGPWQFHLIALSYFLFGASDFTARIPAAL
                     FSIATVWMAWYWRRYLGTWGAIIAGLMMVISPFMLFYGRYVREDVYAGFSGVLMLYAI
                     LRYFEKGTSKYLYLVTIALIIHFLDKESSFIYALQAFLFLAIYFIARVTRKSWNENPG
                     LYRAFIIALAVAVVFVAIGGGFAVANRHTETLASGETAAPVNPDGEGAIPSEITSNSP
                     SPTTLLLGAGGLALIAAAFFLLRGLGWERIRQERSFDLMILLGTFVLPMAIPFPIKWL
                     ESRLHIAIPTDSASVASLTQRDFLYMGLFVVIAFAISIAIGMFWNRNWWKYATLFWGA
                     FTIFYTTVFTNGPGFFTGLLGSLGYWLV"
BASE COUNT          638 a          602 c          543 g          634 t
ORIGIN      
        1 cagggtatca ggagaaaaaa tccttcaaat cctggctgta taccatcaca atcaacacag
       61 ctcgcagtca cttacgcaaa cacagattcg ctgaacgact gcggcagaca ctcacatcca
      121 tatttctggt tgagtctcaa aaatcgcctt cgcctgagga cgtaacgatt caaaatgaac
      181 aggaagcaca attatggaag gcattgaatg agatggatga aaaacaccgg atggttactg
      241 tgctgcgata ttttcatgag ttatctatca cagagattgc ggaaatactt tcgatccatg
      301 aagggacggt caactcccgg cttcatacaa ctcgggaacg actacggaac accctggagc
      361 acttagacaa aaagtaaact gacatgcaaa ttacacatgc agaggcacat aagttaattc
      421 aatttaatct ggatcgaaca ttagatcctg aaaaacaaac gtcccttttt gcgcatcttg
      481 aaagctgtgc gagctgtcgc ggctatgcag atacattgcg aaatgttgaa aatcagttgc
      541 ggcaggtaat gaacaaacaa tggaatcaac ctcccctgcc cctctcgatt gatctcctta
      601 aaacgcaact caagccaccg aggggtaccc aaaacctgtt tcaattgcgc actgcactgg
      661 tcagtatggt tttggttgca ttctcgtttt tcatatggca gttagcctcc ccttcatcta
      721 atccatcaat tcaatcccca tcgacgattt tcccggttcc gacaccatca acgcaattaa
      781 gcacagtaag tatggaaccc acaaaatgtg aatggatgct gtatgaagta aaagcgacgg
      841 acacactcga tactatcgcc ttcaaatatt cggtttccaa gcaagacatc atggagttca
      901 acaacatggg ctctgagatc gtcaacgaaa caatggaatt aaaaattcca caatgcagtg
      961 caacacccac ggtgacggct caacttccga caaccacgct gaccccaaaa cttgagctgt
     1021 tcatcgatac tccgggataa aagcaatgtc attaaggaag tattgtataa tcgtgcctac
     1081 acgaagggta ttaacttagc ttgcacaaag ctatggtata tacagtcaaa ataactttta
     1141 taacgagaga caatcatgga gaaaaaatca actacctggc ttgagcgtcc tgtccatcct
     1201 gcactgccag cgatcaccaa tgaaattgcg atctttgcag gaataattct cctggcaatc
     1261 atcaccaggt tttacgacct tggcacgcgt gtgatgagtc acgacgaaag cctccacacg
     1321 tacttctcct ggctgctcta tcgcgggcag ggttaccagc atacgccgat gatgcatggt
     1381 ccctggcagt ttcacttgat tgccttgagt tactttttat ttggagcaag tgactttacg
     1441 gcacgcatcc ctgccgcatt gttcagcatc gccacggtgt ggatggcatg gtattggcga
     1501 cgttatcttg gaacttgggg tgcgatcatc gccggtctca tgatggtaat ttctcctttc
     1561 atgttgttct acggtcgcta cgtacgcgaa gatgtctacg caggcttctc aggcgttttg
     1621 atgctatacg ccattttgcg atactttgaa aagggaacga gtaaatatct gtatctggtg
     1681 accatcgcgc tcatcatcca ctttctggat aaggaatcct cgttcatcta tgccttacag
     1741 gcatttttgt tcctggcgat ttactttatt gcacgcgtaa ccagaaaatc ctggaatgaa
     1801 aaccctggtt tgtatcgagc tttcatcatc gccctggctg tggctgtcgt atttgtagcc
     1861 attggcggag ggtttgccgt cgcaaaccgt catactgaaa cattagcaag cggtgaaacg
     1921 gctgcacccg taaacccgga tggagaagga gcgatccctt cggagattac gagtaattca
     1981 ccatctccaa ccaccctgct gcttggcgcg ggcggactgg cattgatcgc cgctgccttt
     2041 ttcctcttac gcggactggg atgggagcga atccggcaag agaggtcatt tgacctgatg
     2101 atactgctgg gcaccttcgt cttgcccatg gctatcccct tcccgatcaa atggttagag
     2161 tccaggcttc acatcgcaat ccctactgac agcgcgtcag ttgcctcgct cacgcagagg
     2221 gatttcttat acatggggct ctttgtcgtc atcgctttcg caatctccat tgcaatcggt
     2281 atgttttgga atcggaattg gtggaaatac gcaacattgt tttggggtgc gttcacaatc
     2341 ttctatacca cagtcttcac gaacggaccc gggttcttta ccggcttact tgggtcgctt
     2401 gggtactggc tggtgca
//
LOCUS       DASTJP010000864         4298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_716287, whole genome shotgun sequence.
ACCESSION   DASTJP010000864 DASTJP010000000
VERSION     DASTJP010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716287"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VFQ23_25045"
     CDS             complement(<1..1161)
                     /locus_tag="VFQ23_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909939.1"
                     /translation="MFSDYGKELLKNGIIEAKAGHKEAARRYLDRSIYSSNDHDVLAE
                     AWFWMSEVLQEASEKRKALENCLANNLQHARARRSLAILDGKLRADQIIDPNNLPLAP
                     EGLRSADADRFMCPKCGGRMSFSPDGQSLVCEYCSRNQKFTAQPGYANEKDFIVAMVT
                     ARGHSKPLNQQVFHCEGCGCEFILPPHQISTTCVYCGSPHVVNWESEEQLLAPDGIIP
                     HKFNKEQAAKLLINWVESNKIKPDGGAGLPRPYRTRGVYLPLWTFDLGGMIDYVGETV
                     DENSIQFQGRQPRKIRVSDSYPVQINDLPIPASRKLSAVFLKLIPTFELKAIKPYDPR
                     YLADWPAEVYDIPMAEASLDARSHGFKHYKQDLPNLLSPMQVISTSSAKMSIE"
     gene            complement(1210..2094)
                     /gene="folP"
                     /locus_tag="VFQ23_25050"
     CDS             complement(1210..2094)
                     /gene="folP"
                     /locus_tag="VFQ23_25050"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET9909940.1"
                     /translation="MKPLQIGNHIFNWGSRTYVMGILNVTPDSFSGDGLILPLPQGEG
                     AVRALQQAKEFLSNGVDILDVGGESTRPGSQPVSEEEELQRVIPVIEAITKEFPAALI
                     SIDTYKAKVAEAAFKAGAHILNDVWALRADPDLASVAAAFRVPVILMHNRSNPASVEV
                     REQLGNAYIGSTYENLMEDVKRELLASVDLAVQAGVDESHIILDPGIGFGKTREQNLE
                     LINRLDEIRALGYPVLLGASRKSFIGFTLDLPADQRVEGTAATVAIGIARGADIVRVH
                     DVKEMVRVAKMTDAIVRK"
     gene            complement(2126..2548)
                     /locus_tag="VFQ23_25055"
     CDS             complement(2126..2548)
                     /locus_tag="VFQ23_25055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909941.1"
                     /translation="MSEIKPLNWIPKPGVGFSVVRRDDGGMNLTFTDLSDSTLNDWRG
                     FAMDHLLGSDRRTRNLYDLRAVQEIPEKAIRAAVEANSDPSARNIRVAVVVADEKVAD
                     AVRNVAALALAGSASAMKIFTDIDDAESWLSRSLDQIP"
     gene            complement(2591..3085)
                     /gene="folK"
                     /locus_tag="VFQ23_25060"
     CDS             complement(2591..3085)
                     /gene="folK"
                     /locus_tag="VFQ23_25060"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET9909942.1"
                     /translation="MHEHIVYVALGSNLGDRLVNLKQAIASLSPQIEVRAKSRVYETP
                     PWGHTDQPAFLNQVIKGNTYVEPVPLIKHLKRLEIALGRVPSFQNGPRLIDLDILFYD
                     ELILNTPPLLLPHPRLQERGFVLMPLSDIAPNLVHPTLRKTVSQMLESCDTNGIQVYE
                     KSDK"
     gene            3308..3742
                     /locus_tag="VFQ23_25065"
     CDS             3308..3742
                     /locus_tag="VFQ23_25065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909943.1"
                     /translation="MFAIFLVGCTSSNEQWIIFTQRRAEQMGIGSWISSDGELDGYWT
                     PSAENIQSLEGDLASFLRQNADSFRRQPPVWEQLDQYKRQYVGVIIGGKQVIYGNFFC
                     TETGVDWREDWVLVMDGGDCFFQIQFDVENGTFTSLTVNGEA"
     gene            3789..4103
                     /locus_tag="VFQ23_25070"
     CDS             3789..4103
                     /locus_tag="VFQ23_25070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909944.1"
                     /translation="MESEVAIVDYTSDYQSSIRKILQQIGWAEQFALAKKARGIYVDT
                     PTLNTGGRSFYEAAGYQLGYIMPRYYEDEMDGVTYQKFWRAYEPDEHGKTHACRLASS
                     EK"
     gene            4054..>4298
                     /locus_tag="VFQ23_25075"
     CDS             4054..>4298
                     /locus_tag="VFQ23_25075"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HET9909945.1"
                     /translation="MSTAKPTRADLLAAKNKTIRDVIAPDLLVLFCGINPGLYSGATG
                     HHFARPGNRFWPTLYQAGFTPRLLVPAEERELLDSGYG"
BASE COUNT         1063 a         1066 c         1060 g         1109 t
ORIGIN      
        1 ttcaatgctc atcttcgcag acgaggtcga gatgacctgc atcggagaca gcaaattggg
       61 aagatcttgt ttgtaatgtt taaaaccatg actgcgcgcg tcgagggatg cttctgccat
      121 cggaatgtca tagacttcgg ctggccagtc cgccaggtaa cgcgggtcgt atggtttaat
      181 agctttcagc tcgaaggtgg gtatcaactt cagaaaaaca gcagaaagct tccgtgacgc
      241 gggaatgggc aggtcattga tctgcaccgg atagctatca ctgacgcgga tcttacgcgg
      301 ctgcctgccc tggaactgaa tgctgttttc gtccactgtt tcacccacat aatcgatcat
      361 tccacccaaa tcgaacgtcc ataggggcag ataaacgccg cgagtccggt aagggcgggg
      421 taaccccgcc cctccatccg gcttgatctt attagactca acccagttaa tcaacagctt
      481 tgcagcctgc tccttgttga acttatgcgg aatgatccca tcgggtgcca gcaattgctc
      541 ctctgattcc cagttgacga catgcggcga accgcaatat acacaagtag ttgagatttg
      601 atggggaggg agaatgaatt cacaaccgca gccttcgcaa tgaaaaacct gctggttcaa
      661 cggcttgctg tgcccgcgtg cggttaccat cgcaacaatg aagtcttttt catttgcata
      721 gccgggctgc gcagtgaact tctgattgcg cgaacaatat tcgcaaacca gtgattggcc
      781 atctggggaa aagctcatcc gcccgccgca cttgggacac atgaaacgat cggcatctgc
      841 cgagcgtaac ccttcgggcg caagcggaag gttattcggg tctatgattt gatctgccct
      901 cagtttgcca tccaaaattg ccagggaacg tcttgcccgc gcgtgctgta agttatttgc
      961 cagacaattt tcgagtgcct tgcgtttttc agaggcttcc tgaaggactt cactcatcca
     1021 aaaccatgcc tcggcaagaa catcgtgatc attgctcgaa taaatcgatc ggtcgagata
     1081 acgacgcgca gcctctttat gtcccgcttt tgcttcgatg atgccatttt tgagaagttc
     1141 tttgccgtag tcgctgaaca tcgtttttct ttctgtagcg aatggcagct gccgttcgct
     1201 acaacaatat tatttcctca caattgcatc tgtcatcttt gctacgcgca ccatttcttt
     1261 tacatcatgc acccgcacga tatctgcgcc gcgcgcgatg ccgattgcga ctgtcgccgc
     1321 agtgccttcc acgcgctggt ctgcaggcag gtcaagtgta aagccgatga atgactttcg
     1381 ggatgccccc aaaagtacag gatagcccag tgcccgaatc tcatctagtc tgttgatcaa
     1441 ctccagattt tgttcgcgcg tctttccaaa accgataccg ggatccagaa taatatgcga
     1501 ctcatccacc ccggcctgca ccgccagatc tacgctggcg aggagttcac gtttgacgtc
     1561 ttccattaag ttctcatacg tcgagccgat atatgcgttg cccaattgct cacgaacctc
     1621 tacgcttgcg gggttgcttc ggttatgcat caatatcaca gggacgcgaa atgctgcggc
     1681 aacagaggcc aggtcagggt ccgcgcgaag tgcccagaca tcgttgagga tgtgtgcgcc
     1741 cgctttgaag gctgcttctg cgaccttcgc cttgtacgta tcaattgaaa ttaaagcagc
     1801 cgggaattct tttgtgatcg cttcgatgac cgggataaca cgttgtagct cttcctcttc
     1861 agaaaccggt tgagaaccag gtctggttga ctcacctcca acgtctaaaa tatccacccc
     1921 attggaaaga aactcttttg cttgttggag ggcgcgtact gccccctctc cctgcggcaa
     1981 agggagaatt agtccatctc cactaaaact atctggcgtg acgttaagga ttcccatcac
     2041 gtaggtgcgg gaaccccagt tgaatatgtg gtttccgatt tgcagaggct tcatgattcc
     2101 tgctcgaaca gtctggagca gggtgctatg gaatctggtc cagtgaacgc gagagccagg
     2161 attctgcgtc atcaatgtct gtaaagattt tcatggcaga ggcgctcccg gccagcgcca
     2221 gtgcagccac atttcgaact gcgtctgcga ctttttcatc tgcaacaacc acagccacgc
     2281 gaatatttcg agcggatgga tcactgtttg cttctactgc tgctcgaatc gctttctcag
     2341 ggatttcctg cacggcgcgc aggtcatata gattgcgtgt acggcgatcg gagccaagta
     2401 aatggtccat tgcaaagcca cgccagtcat tgagcgttga atctgaaaga tctgtaaaag
     2461 ttaagttcat gccgccgtcg tcccggcgta caacagaaaa cccgacaccg ggtttgggta
     2521 tccagttaag aggtttaatt tcgctcatgg tttgcctcca aataagtata cagggattat
     2581 acacgctgca ttatttatca cttttttcat acacctgtat cccatttgta tcacaggact
     2641 ccagcatttg acttaccgtc tttcgaaggg tgggatgcac aagattcggc gcaatatcac
     2701 taagaggcat caacacgaat cctcgctcct gcaaccgagg gtgtggcagg aggagaggtg
     2761 gcgtattcag aatcaattca tcatagaaga gaatgtcaag atcaatcaga cgaggtccat
     2821 tttgaaagct gggtacgcgc cccaacgcaa tctcaagtcg ttttagatgc ttgattaaag
     2881 gaacgggctc tacatacgta ttccctttga tgacctgatt gaggaatgct ggctgatctg
     2941 tatgccccca gggtggggtt tcatatacgc gcgattttgc tctcacttca atttgtggag
     3001 ataaggaggc aatggcctgt ttcaaattca ccaggcggtc acccaggttg gagccaagag
     3061 ctacataaac aatatgttcg tgcatgattt atcctgtaca gagacaagtc tccgcttaaa
     3121 tgctatactg caaaatgcta cgtagtcatc cgttgtgacg gagttaaact cagcgaatta
     3181 agccgtaagg caagtgattt ctaaacgcat tgtacccttt aacaagggaa aacgattgaa
     3241 tcaagcaagc tcattaaagt atatgatgaa ctatcataaa ttcattttat ttttcgctag
     3301 ctttagtatg tttgcaatct ttctcgtcgg atgtacctcc tcgaatgaac aatggatcat
     3361 cttcacccaa cggcgtgcag agcaaatggg aatcggctca tggatatcgt ccgatggaga
     3421 gttggatggc tactggactc cttcagcaga gaatatccaa agccttgagg gcgacttagc
     3481 ttccttcctt cgccaaaatg ccgattcgtt caggcgccag ccaccggtat gggaacaact
     3541 tgaccagtac aagcggcaat atgtgggcgt gattattggt gggaaacagg tcatctatgg
     3601 aaatttcttt tgcactgaga ccggtgtgga ctggagagaa gactgggtcc tggtgatgga
     3661 cggaggagat tgtttctttc aaatacaatt cgacgtagaa aatggcacct ttaccagctt
     3721 aacggtaaat ggcgaagctt aataacagtt ttggaacttg aagaattcaa cgagttaact
     3781 aagtaatcat ggaatctgaa gtagccattg ttgattacac atccgactat cagtcctcca
     3841 ttcgcaaaat actccagcag atcgggtggg cggagcagtt cgcacttgct aagaaggcgc
     3901 gtgggattta tgttgacaca ccaaccctaa atactggcgg acgtagcttc tacgaagccg
     3961 ctggttatca actcggatac atcatgcctc gttattatga ggatgaaatg gatggggtaa
     4021 cgtatcaaaa gttttggaga gcctatgaac ctgatgagca cggcaaaacc cacgcgtgca
     4081 gacttgctag cagcgaaaaa taagacaatt cgcgatgtaa ttgcgcctga ccttctcgtg
     4141 ctgttctgtg gcatcaaccc cgggctatac tcaggagcga cgggtcacca ctttgcgcgt
     4201 cccggaaatc ggttttggcc tacgctgtat caggctggtt ttacccctcg tttgctggta
     4261 ccagcggagg aacgcgaact gctggattcc ggatacgg
//
LOCUS       DASTJP010000865         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1574475, whole genome shotgun sequence.
ACCESSION   DASTJP010000865 DASTJP010000000
VERSION     DASTJP010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574475"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..799
                     /locus_tag="VFQ23_25080"
     CDS             137..799
                     /locus_tag="VFQ23_25080"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET9909946.1"
                     /translation="MRALIFDLDGTLIDTVYAHVFAWQRALAEAGMAIEGWRIHRKIG
                     MSGGLFTRAVAREIGRDLSSSEEKALQNRHGELFTQLMPTRQPLQGAVDLLSFLRAHQ
                     ILFGIATSGRRPEINASLEILGIGSDTVVVERGDVARAKPEPDLFLACQQRLGVSASD
                     CYVVGDAVWDLLAARRAGMLSIGLLSGGYGEDELSRAGAFRVYRNPAELLGSLDELGV
                     LP"
     gene            796..2439
                     /locus_tag="VFQ23_25085"
     CDS             796..2439
                     /locus_tag="VFQ23_25085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909947.1"
                     /translation="MNSDFFARDLSGIIFLVLLIALPLAYLICLVLLRLYRRAVLQVM
                     LQQKENTQKETSSKPFVIPSDETGKRELKITVHDEKSEGIATSSPQHSHTNLLGAPRR
                     AAIVYSIAGLSYALVMTIVFLSATRSGFHLTSFLMLFWYYSWPVVVTVCLVAAANWQM
                     RLSVTLIYFLALIPLLILTFFVRPVVGWGQIAVLWLLTNLVALIILLAFLNRRIRAVG
                     PFVLTFMIIAVTGSVILPIFLGMNSILFAVLGFIVFGMLGWLLVQGIGNWYKQKKISE
                     QSITIDAIWLLFGMFQSIGLLFEGERWFIASLMAFVVYKLVSAVGFSYFGRKVSSDQN
                     SPKLLLLRVFSLGPRSERLFDILGIYWRYVGSIRLIAGPDLATATLEPHEFLDFLSGK
                     LSRRFIDTAQTLDLRISEMDSRPDRDGQFRVNEFFCYDATWKMVLLRLVGTSDVVLMD
                     LRGFSSQNAGCIFEINELINTVNPARIIFVIDETTDETFLRKVLQQAWDEMRATSPNV
                     ALKTGVLHLFRLKGFGNRDLKHFLHKLGSNVTTETKLTG"
     gene            2475..3746
                     /locus_tag="VFQ23_25090"
     CDS             2475..3746
                     /locus_tag="VFQ23_25090"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9909948.1"
                     /translation="MTETNTNWARNYVYRAAKLHRPTTIEQVQELVSRSTKVKALGSR
                     HSFNDIADSTGDLISLEHLNHVLELDQDRRTVTVEAGVRYGELGQWLHERGYALHNLA
                     SLPHISIVGACSTGTHGSGDQNGNLATVVSSMEMIKADGEIIAFSREKNPEDFHGAVV
                     GLGGMGLITKLTLDIIPTFQIRQDVYENLPLTQLEDHFDELSSSAYSVSLFTDWRNAT
                     FNQVWLKSLDSDESSFNAEPTLSGATRATRRLHPIASISAENCTEQMGIAGPWHERLP
                     HFRMDFTPSSGEELQSEYLIPRQYAFDVYKAIDGLRQHIVPLLQISEVRTIAADSLWM
                     SPCYHQDSVAIHFTWKKDWAGVSQILPILEDALAPFHARPHWGKLFTMHPSQLQSLYE
                     KLPQFQELLRLYDPEGKFRNPYLDKYILGDA"
     gene            3776..4621
                     /locus_tag="VFQ23_25095"
     CDS             3776..4621
                     /locus_tag="VFQ23_25095"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9909949.1"
                     /translation="MFKVFIALSVLIVVLGFAEVNNALHPPRIIPPENTLRENDIEYQ
                     QLDLVTEDGIRLSAWYTPPRKGAVILLAHGYGDNRPEWVHALLAKKGYGVLAWDARAH
                     GESDGEVSTIGYLEVLDVKAALDFALNQPEVLHVGAWGGSMGGATLIRAAAAFPEIEA
                     VFIDSAFASLNDEFDFLVPYPVINPLAKFLAEKRTGISLDQINPLNDIGQISPRPVYI
                     VHGTGDTVAPPNAGEKLYNAANEPRFLWIEEDAAHLSIHLVNERRYQRRLIGFFDEWL
                     LGQQE"
BASE COUNT         1147 a         1166 c         1208 g         1306 t
ORIGIN      
        1 caaattgatt gccgcgggag tggatacgaa aggtgtcgag cagtttgttc ctacggggaa
       61 ctggtacgtc gcctttcgcg acccggataa cattcaactg gaatactggc tgccttaatc
      121 gtttcaggag aatcctatga gagctctcat ctttgacctg gatggtacgc tcattgatac
      181 agtctatgcc catgtgtttg cctggcaaag agcattagcc gaggcaggta tggcaatcga
      241 aggctggcgc attcaccgaa aaatcggcat gagcggcgga ctgtttaccc gcgcggtggc
      301 gcgtgaaatc ggacgtgacc ttagttcatc cgaagagaaa gcacttcaaa accggcatgg
      361 agaacttttt acacaactca tgccgactcg tcaaccattg caaggcgcgg tcgatctatt
      421 gagcttcctc cgtgcgcatc agattctctt tgggatcgcc acctctggtc gccgcccgga
      481 aatcaacgct tcattagaaa tcctcggcat tggtagtgat acggtggtcg tggagcgtgg
      541 agatgtcgcc cgcgcaaaac cggaaccaga tctcttcctt gcctgtcaac aacgtcttgg
      601 cgtcagcgca agtgattgtt acgtggttgg ggatgcagtt tgggatcttc tggctgctcg
      661 acgagcaggc atgttaagta tcggtttgct gagcggcgga tatggcgaag atgaattgag
      721 ccgagcgggt gcatttcggg tatatcgaaa ccctgcggag ttgcttggct cgttggatga
      781 actgggtgtc ttaccatgaa ttccgatttc tttgcacgcg acctatcggg cattattttt
      841 ctggttcttt taatcgccct acctcttgct tacctgatat gccttgttct tttgaggctc
      901 tatcgccgcg cagtgctcca ggtaatgctt caacaaaaag aaaacacaca gaaagaaact
      961 tcatcaaaac cgttcgtaat accttctgat gaaaccggaa aaagagaact gaaaatcacc
     1021 gttcatgatg aaaagtcaga aggcatagct acctcatctc cacagcacag tcatacaaat
     1081 cttctcgggg caccacgccg tgctgctata gtttattcaa ttgccggact ttcctatgca
     1141 ttggtgatga cgattgtttt tctatctgcc acgagaagtg gatttcacct gacctccttc
     1201 ctgatgttat tctggtacta cagctggccg gttgttgtca cagtgtgtct cgtggctgct
     1261 gcaaactggc agatgcgatt gtctgtcacc ctaatttatt ttcttgcact tattccgctt
     1321 cttatattga ccttctttgt taggccggtc gtcggctggg gtcagatcgc tgtgctatgg
     1381 ctgctgacaa atcttgttgc tcttattatt ttgttagctt ttctcaatcg aagaattcgg
     1441 gcggtcgggc cgttcgtact gacttttatg atcatcgcag tgacaggttc cgtcatcttg
     1501 cccatttttc ttggaatgaa ttcaatcctc tttgctgtcc tgggcttcat tgttttcggc
     1561 atgcttgggt ggcttctggt tcaaggaatt gggaattggt ataagcagaa gaagatcagc
     1621 gaacaatcca ttacgattga tgccatctgg cttctttttg ggatgtttca gtcaattggt
     1681 ttgctctttg agggagagag atggttcatt gccagcctga tggcttttgt cgtttataaa
     1741 cttgtttccg cagtcgggtt ttcctatttt ggcaggaaag tatcctccga ccagaacagc
     1801 ccgaagcttt tactgctccg agtattttcg ctcggacctc gtagcgagcg cctgttcgac
     1861 atccttggta tctactggcg atatgtagga agtattcgtc tcatcgccgg acccgatctg
     1921 gcaaccgcca ctctggagcc gcacgaattc ctggattttc tcagcggaaa actgtcgcgc
     1981 cgattcatcg atacggctca gactctcgat cttcgaatct ctgaaatgga tagccggccg
     2041 gatcgggacg gccagtttcg agtcaatgag ttcttttgtt acgacgccac ctggaaaatg
     2101 gtccttttac gcctcgtggg gacgagtgat gttgttctaa tggacctgcg tggattctca
     2161 tcgcaaaatg caggctgtat ttttgaaatc aatgagttga tcaacacggt gaacccggca
     2221 aggatcatat ttgtcatcga tgagaccacc gatgaaacgt tcctccggaa agtgctacaa
     2281 caggcatggg atgagatgag ggcaacctct ccaaatgttg ctttgaaaac tggggtactc
     2341 catctttttc gccttaaagg attcggcaat cgtgatctga aacacttttt gcacaagctt
     2401 ggttcgaatg tcacaactga aacgaagctt acaggttaaa aaagcagagt tcctttgcta
     2461 tatgggagta ggccatgact gaaacaaata caaattgggc aaggaactac gtttatcggg
     2521 ccgctaaatt gcacagaccc accacgattg aacaggtgca ggagcttgta tctcggagca
     2581 ccaaagtaaa agcgttgggt agcaggcatt cgtttaatga catcgccgat tcaaccgggg
     2641 atttaatttc gcttgaacac ctaaatcatg ttttggaatt ggatcaagat cgtcgcactg
     2701 taaccgttga agcgggggtg cgttacggag agctaggtca atggcttcat gagcgagggt
     2761 atgcgcttca caacctggca tctttgcccc acatttcaat tgttggcgcc tgctccaccg
     2821 ggacacatgg ctcaggtgac caaaacggaa atctagcgac tgtggtttca tccatggaaa
     2881 tgattaaagc ggatggtgaa atcatcgcgt tctcacgtga aaagaatcct gaagattttc
     2941 acggtgcagt tgttggcttg ggaggcatgg gcctaatcac gaagctgacg ttagatatta
     3001 ttccgacttt tcaaatacgg caggatgttt acgaaaatct tcctctcaca caactggaag
     3061 atcacttcga tgaactttcg tcaagtgcgt acagcgtcag cttgttcacg gattggcgga
     3121 atgctacgtt caaccaggtc tggttgaaaa gcctggactc cgatgagtct tctttcaacg
     3181 cagaaccaac cttgtccgga gcgacacgcg ctacgagacg acttcacccc attgcaagca
     3241 tctctgcaga gaactgcacc gaacagatgg gcatagccgg accctggcac gagcgccttc
     3301 cacacttccg catggacttc acacccagca gtggcgagga attgcaatca gaatatctga
     3361 tacccaggca atacgcgttc gacgtttaca aagctattga tggattacgt caacatatcg
     3421 ttccgcttct gcaaatttca gaggtacgca cgatcgccgc agatagcctc tggatgagtc
     3481 cctgctatca tcaggattcg gtcgctatcc actttacgtg gaaaaaggac tgggctgggg
     3541 tcagtcagat tttgccgata cttgaagatg cgctggctcc atttcatgcg cgcccgcatt
     3601 gggggaagct ctttacaatg catcctagtc aattgcaatc cctctacgaa aagctgcctc
     3661 aatttcaaga actacttcgc ctttatgatc cagaaggtaa atttcgtaat ccatatctag
     3721 ataaatatat tttgggtgac gcgtgaagaa caatgtacga ataacattcg gaattgtgtt
     3781 caaagtattt attgctttgt cggtgttgat cgtcgttctc ggatttgccg aagtgaataa
     3841 cgcgttgcat ccaccgcgca tcatcccacc agagaatact cttcgtgaaa atgacattga
     3901 atatcaacaa ctcgatctgg tcactgaaga cggaattcgt ctttcagcct ggtacacccc
     3961 tcccagaaag ggtgctgtaa ttctgcttgc acacggctac ggcgacaacc gccccgaatg
     4021 ggtccatgcg ctgctggcaa agaagggtta cggtgtcttg gcctgggatg cgcgagccca
     4081 tggtgaaagt gatggtgaag tctcaacaat tggctatcta gaggtgctgg atgtaaaagc
     4141 cgcgctggat tttgcattga accagccaga ggtacttcac gtcggcgcct ggggcggctc
     4201 gatgggtgga gccacgttga ttcgcgccgc cgcggccttc cctgagattg aagcggtctt
     4261 catcgacagc gcatttgcat ccctcaacga tgagtttgac tttcttgtcc cgtaccccgt
     4321 gattaatcca ctggcgaagt tcctcgcgga gaaacgaacg ggtatctctc ttgatcagat
     4381 caatccattg aatgatatcg ggcagatcag cccacgccct gtctatatcg ttcatggcac
     4441 gggcgatact gtcgctccgc ctaatgcggg cgagaagcta tacaatgccg ccaacgagcc
     4501 acgtttttta tggattgaag aggacgcagc tcacctttct atccacctag tcaatgagcg
     4561 tcggtatcag cgaaggctga tcggtttttt tgatgaatgg ctgctgggcc aacaagagtg
     4621 atcatcgccc tgcaacctac gggttagagg gcgatgaccg aatgatcgtt ttactactgt
     4681 tcaattaccc agacttccgc attgctaatc tcgcagcgtg tcccaaaatc tgtacctgag
     4741 ccggtcagga tcaggcttga taaaggtccc gcttccgtga gccagtcatt tttcaacttg
     4801 tgctcagtaa agaaatcacc gttgatg
//
LOCUS       DASTJP010000866         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_400465, whole genome shotgun sequence.
ACCESSION   DASTJP010000866 DASTJP010000000
VERSION     DASTJP010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400465"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1272)
                     /locus_tag="VFQ23_25100"
     CDS             complement(130..1272)
                     /locus_tag="VFQ23_25100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909950.1"
                     /translation="MKYILSFLLLISFLTACAAPTERTPTPYPDTPSPAEITAPLVES
                     PALISIQFLNELDGWGVTETQIVRTNDGGITWYNVTPPNLTEAGYSVEADVLDTNHAW
                     VQKTNFDGDPNSGFLYRTSDGGNTWTESTTPFGGGDLNFIDANNGWMLTSLGVGAGSM
                     GISVFQTADGGATWEQKYTNDPNLANAGDSLPLSGLKNGLAPLDAQNAWVYGVVYAPG
                     NLYLFRTNTGGENWTPVPIQLPPGAENSEVGIDRDQMRFVSATNGFMAVRMVGDATQT
                     AVYTTRDSGNNWALSPTLIPNGGASDFISSEEAVLYNGEQFYVTRDAARTWMIIPPDI
                     KFGETFAGMDFVITLSGWVITLDPTNNQRTLYRTSDGGATWFPVVQ"
     gene            complement(1325..1723)
                     /locus_tag="VFQ23_25105"
     CDS             complement(1325..1723)
                     /locus_tag="VFQ23_25105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909951.1"
                     /translation="MVTEMFPEYSTGLDAFQKPHMNIYQVFFTFESDIDGLIQAKDQE
                     SVAKIFKFTEWCFDQRKRNHDIWNAAATAFLEHLADKDERAEIIPFWIKPEIFLDMKS
                     EFKKRRERDGKGKFQTLVEKYNQINNTNFE"
     gene            complement(1793..>2103)
                     /locus_tag="VFQ23_25110"
     CDS             complement(1793..>2103)
                     /locus_tag="VFQ23_25110"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET9909952.1"
                     /translation="IPATRKALQRAGLQVKDIRLVELNEAFAVQSLAVMEDLELEPDI
                     VNVNGGAIAIGHPLGCSGARLVTTLVHEMRLRGDVKYGLATLCVGVGQGEATILEYLG
                     "
BASE COUNT          537 a          534 c          482 g          550 t
ORIGIN      
        1 tgtcctgggt aagcacggga gttgataaag tgggaacagg cgttgcctga gtcggatttg
       61 agggagtcaa taaatctgag aagaaatctc cgcatgaggt caaagccaga catacaatca
      121 cgatccagct tattgaacga cggggaacca tgtggcgcct ccatcgcttg tacggtacag
      181 cgtgcgttga ttattggtcg ggtccaaggt gatgacccag ccagacaacg tgatgacgaa
      241 atccatacct gcgaacgtct caccaaattt aatatcggga ggaatgatca tccatgtacg
      301 cgccgcatcc cgcgtgacgt agaactgctc gccattgtat aggacggctt cctccgaaga
      361 gataaaatcg gaggcacccc cgtttggaat cagagtcggt gaaagggccc agttgtttcc
      421 agagtcgcgc gtagtgtaaa ccgcagtttg agtagcatct cccaccatgc ggaccgccat
      481 gaatccattc gtagcggaca caaatctcat ttgatcccgg tcgatgccca cctcagaatt
      541 ctcagcacca ggaggcaatt ggatgggaac gggcgtccag ttttcgcctc ccgtattcgt
      601 tcgaaagaga tacaaattgc ccggagcata aacaacacca tatacccagg cgttttgcgc
      661 atccagaggg gcaagtccat tcttaagtcc actgagcggg agcgaatcgc ctgcattcgc
      721 cagattggga tcattcgtat atttttgttc ccaggttgct cccccatctg ctgtttgaaa
      781 aacggatatg cccatcgagc cggcgccgac gccaaggctg gtaagcatcc aaccgttgtt
      841 cgcatcgatg aagttaagat ctcctccacc gaatggagtc gtagattccg tccacgtgtt
      901 gccaccgtca gatgtccggt aaagaaaacc gctgtttgga tcaccatcaa agttcgtctt
      961 ctgcacccag gcgtgattgg tatcaaggac atccgcctca acactatagc ctgcctctgt
     1021 aaggttcggc ggtgtaacgt tgtaccaggt gattccccca tcattggtgc ggacgatttg
     1081 tgtctcggtc acaccccagc catccagttc gttgagaaac tggatagaga ttagcgcggg
     1141 ggactccacc aaaggcgccg taatttcggc tggcgaaggt gtatcaggat acggagttgg
     1201 agtgcgttct gtaggagcag cgcaagctgt cagaaaagaa ataagaagga ggaaagagag
     1261 gatatatttc attatgcttc aggattctaa gtttgcatgg gtcactaaaa gattatcgat
     1321 tatgtcattc aaaattcgta ttatttatct gattgtactt ctctacaaga gtttgaaatt
     1381 ttccttttcc atcgcgctcg cgccgcttct tgaattcgga tttcatatcg agaaaaattt
     1441 caggctttat ccaaaatgga attatttcag ccctttcatc tttatccgct aagtgttcta
     1501 aaaatgcagt ggctgcagca ttccaaatat catggttgcg cttcctttgg tcaaagcacc
     1561 actcagtaaa cttgaaaatc tttgccacgc tttcctggtc ttttgcttga atcaacccat
     1621 cgatatcact ttcaaacgtg aaaaatactt gataaatatt catatgaggc ttttgaaacg
     1681 catccaatcc tgtcgaatat tcaggaaaca tttcagtaac cattcttttc caggtactca
     1741 tcgattcttg ttcaccgtga tgcttattcc tttgacttgc aactttcgac cttcaaccta
     1801 gatattcaag aatcgtcgct tcgccctgcc ccaccccaac acagagtgtc gcaaggccat
     1861 actttacatc tccacgtaag cgcatttcat gcacaagtgt cgttaccagg cgcgcgcctg
     1921 aacatccgag tggatgccca attgcgatcg cgccgccatt tacattgact atatctggtt
     1981 cgagctctaa atcttccatc accgccagcg actgaaccgc gaaggcttcg ttcaactcta
     2041 ccaaacgaat atctttcacc tgcaagccag cacgctgtaa tgcttttctt gtcgcgggga
     2101 tag
//
LOCUS       DASTJP010000867         6786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1146621, whole genome shotgun sequence.
ACCESSION   DASTJP010000867 DASTJP010000000
VERSION     DASTJP010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146621"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFQ23_25115"
     CDS             complement(<1..692)
                     /locus_tag="VFQ23_25115"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9909953.1"
                     /translation="MYSFDPTEEQQMLMEAVGKFATNDLRPAAHEAEENRELQKKMLN
                     KGWELGLLQASIPESYGGFGERSAVTGVLALEEMAFGDLASTLAVLTPGLFAMPILLA
                     GSEQQKQLHLPKVIEGTWSPYTAALIEYALDFDPNALRTTATLQNDQYVLNGEKAVVP
                     FANESETLLIYANLDGITQGFIVDKTTTGLTICDEREKLMSLNALPLYRVTLENVRVS
                     VANRLGGESGHD"
     gene            complement(823..1893)
                     /locus_tag="VFQ23_25120"
     CDS             complement(823..1893)
                     /locus_tag="VFQ23_25120"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9909954.1"
                     /translation="MSEQRSNATLETAGGAKIHRISLEVFPDFWAYVYLVLKDELSVL
                     IDCGSGTDSSHAGLLSGLEQAGLTPSDLSHILLTHAHIDHYGGLSKLKPLTDAKVGVH
                     ELDLQTVAHHEAKVALVSRRLGSFLTEAGLSEETCDQLLSIYRFTKALYQSVPVDFTF
                     GQAQGGADEETDVLLNSFEMIHLPGHCPGHVAIRLDDFIFCGDMVVEGVTPHLIPESI
                     EPNNGLDHYLESLNRFQIWANGTRLIFNGHDEVISDLPVRIDSTYQNLIRRMSKALDA
                     LSEPLTTAEVCTAVYGETGGYNQLLVIEKTGAYIEYFYQHGMIGITNTDEVEQGLPPR
                     YRRFKAISETEILPKQKHPVVD"
     gene            complement(1890..2450)
                     /locus_tag="VFQ23_25125"
     CDS             complement(1890..2450)
                     /locus_tag="VFQ23_25125"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9909955.1"
                     /translation="MTREDILEAAAQVFRQKGFHGASMNDIAEAVHLQKASLYHHVSS
                     KQEILLDILDRALQLLLEQISSISNQNILADKKLRLMVRAYLQILIENIDLATVLLFE
                     HRALERRQHARHIPNRDKFESLWKDVIAEGVKSKLFKCDNPALATRALLGIMNWTITW
                     YRPDGELSIEQIADQYSDLLLNGLLK"
     gene            2556..3527
                     /locus_tag="VFQ23_25130"
     CDS             2556..3527
                     /locus_tag="VFQ23_25130"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9909956.1"
                     /translation="MKYFVTGATGFVGGVLAKKLREAGHEVNASVRSPGKTKELQDLG
                     VKIFKGDVTDKESMREAMTGVDGVFHVAGWYKIGVKDKSDGERVNIQGTRAVLELMQE
                     LRIPKGVYTSSLVVNSDTQGELKDESYHFTGQHLSEYSRTKAVAHEMAKEFIAKGLPL
                     VIVMPGLIYGPGDTSILRANIRDFLNQKLPLLPSQNAYCWAHVDDIVQGHILAMEKGR
                     IGESYIIAGEPVTTADAFKLAGEITGKRVPSVVSYNLIKALSVIVRPFDNFLPETFTS
                     EGLRMIAGVTNWGDNSKAKRELGYHPRPFREGWGETLLHEQKLLKQS"
     gene            3546..4001
                     /locus_tag="VFQ23_25135"
     CDS             3546..4001
                     /locus_tag="VFQ23_25135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909957.1"
                     /translation="MSTSEFEQPVPPLVSEPVTSPDDQDKRMRRILIGVIVGAVILVI
                     LLGVSIYLLLQPATPTDRIRDVFIIVVALESLVIGVAMIVLIMQLASLINLLQNEIRP
                     ILHATNETVNTLRGTAEFLGDNVVEPVIKLNGYLAGLYRMLELMGIRKK"
     gene            4087..4503
                     /locus_tag="VFQ23_25140"
     CDS             4087..4503
                     /locus_tag="VFQ23_25140"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9909958.1"
                     /translation="MSERDEFGAFLVGFIVGGLTGSVVALLFAPQSGEETRAVIRDKS
                     IELRDKAQISAEEAIARAEAAAAEARARADELAKQLRERGTEVYGTVRDRGQAVYEDV
                     RERGKAAYDDVRDRSKSAVDNIRSKVKKEEGETAAG"
     gene            4588..5208
                     /locus_tag="VFQ23_25145"
     CDS             4588..5208
                     /locus_tag="VFQ23_25145"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9909959.1"
                     /translation="MKAFLRYFFKLLYHQFAFAYDLVAASVSFNRWKEWVKSVIPLID
                     GNRILEIGHGPGHLHRALLDRGLFAVGIDESKQMGRLAKRRAGSTARLTRGLAQHLPF
                     PSESFDTVISTFPAEYINDPRTLAEVKRCLRSSGRFIILPVAWPKNPFLEWLFRVTGE
                     SPSEALELVKAKFREPFAKAGFSTKVETLDVKSSILLIIVATKLSP"
     gene            5231..5869
                     /locus_tag="VFQ23_25150"
     CDS             5231..5869
                     /locus_tag="VFQ23_25150"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET9909960.1"
                     /translation="MFKKLREPVNSLTHWAGAFLALCGLIALLVVGWGTPAKVISLLI
                     YGVSLIFLFSASATYHMVQVKDKALEIFRKIDHAAIYFLIAGTYTPFCVNAFSGFWQW
                     GMLSIIWSLALIGITIKVFYIRAPRWLNAGIYLVMGWLCLAAIGQMLITLPAWVIGWL
                     VAGGIMYTLGAVVYITKIFNFVPGIFGFHEIWHIFVMLAAAAHFVAVLGVAL"
     gene            5949..6740
                     /locus_tag="VFQ23_25155"
     CDS             5949..6740
                     /locus_tag="VFQ23_25155"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HET9909961.1"
                     /translation="MLFGFDLNQLLLFPIKDDEARKHFIVGCLIYLAGFFIPILPWLV
                     TSGYSAIIIRQVLNGEKPHMVPWDNWEQLFKDGARLFGITLIYSLPLFVLMIFFFLSF
                     FAVPLLPALTKGDNNQGLGIGWFIFMLIGMGVSFLVFPLSLAIGLIVPAAEIHAVAKD
                     DFMAGFRVREWWPIFRQNWQGFVVAMAILYALLTAMSFAMQFLIITVVLFCLLPLLIP
                     VISMYSAIIQFATFAQAYKEGRDKLSVVSAQAPTTGETDLQIQTS"
BASE COUNT         1683 a         1594 c         1722 g         1787 t
ORIGIN      
        1 aaatcatgac ccgattcgcc accaagtcga ttcgccacag aaacccgcac attttccaat
       61 gtgactcgat acagcggaag tgcattgagg ctcatcaact tttcgcgttc gtcacagatg
      121 gtcaatcctg tcgttgtttt atctacgata aagccttgtg taatgccatc aagattggca
      181 tagataagca gggtttctga ttcgtttgcg aaaggcacaa ccgccttttc gccatttaga
      241 acgtattgat cgttttgcag cgtagccgtt gtgcgcaaag cattcgggtc aaagtcaaga
      301 gcgtactcga tcaaggctgc agtatacggg ctccaggtac cctcaatgac ctttggcaaa
      361 tggagttgct tttgctgctc actgcctgcc aaaagaattg gcatggcgaa caagccgggt
      421 gtcagaaccg caagtgtact agccaggtcg ccgaacgcca tctcttcgag cgccagcacg
      481 ccggttaccg cacttcgttc accgaagcca ccataggatt cgggtataga agcttgcagc
      541 aatccaagct cccagccttt gttcagcatc ttcttttgca gttcgcggtt ttcctcggcc
      601 tcatgggcag cgggacgcaa gtcattagtg gcaaactttc ccactgcttc cataagcatt
      661 tgctgttctt cagtcgggtc aaatgagtac atgttctttc cttttcattc atattccgct
      721 ggttgagtag cctgaggcga tagccgatgg catatcgaaa ccagtaagtt acatacaatc
      781 taaaacttca ttgaaaccgg tggtttcgat acgggctctg ccctaatcaa ccaccggatg
      841 tttttgtttt ggcagtattt cagtctcaga aattgcttta aaccttcgat agcgcggggg
      901 caatccctgc tctacttcat ctgtgtttgt gatgccgatc atgccgtgct ggtagaaata
      961 ttcgatataa gcaccggtct tttctatgac cagcagttgg ttgtacccgc ccgtttcgcc
     1021 gtagacagca gtgcagactt ctgccgtggt caatggttcg cttaacgcat ccagggcctt
     1081 gctcatccga cggatcaagt tttgatacgt cgagtcgatt ctgacgggta aatccgagat
     1141 cacctcatca tgtccgttga aaatcaagcg ggtcccgttt gcccaaatct ggaaacgatt
     1201 gagtgattca aggtagtgat cgagtccgtt gtttggttca atggactcgg ggataaggtg
     1261 tggagtgaca ccttcgacaa ccatatctcc gcaaaagatg aagtcgtcca gcctgatcgc
     1321 aacatgaccg ggacaatgcc ccggcaggtg aatcatctcg aacgaattga gaagcacatc
     1381 tgtttcctca tcagcgccgc cctgagcctg gccaaaggtg aaatcaacgg gaaccgactg
     1441 gtacagggct tttgtaaagc ggtaaatact gagaagttga tcacaagtct cttcagagag
     1501 cccggcttcg gtcaggaaag aaccgaggcg ccggctcacc agtgcgactt ttgcttcatg
     1561 atgcgctacc gtttgcaaat ccaattcgtg gactccaact ttcgcatcgg tcagcggctt
     1621 cagcttggag agcccaccat agtgatcaat gtgtgcgtga gttagcagaa tgtgagaaag
     1681 gtctgaaggt gtaagtcccg cttgttccag tccactcaat aagccggcat gagatgaatc
     1741 ggtgcctgaa ccacaatcaa tgagaacgct taactcatcc ttcaatacga ggtatacata
     1801 agcccaaaaa tccggaaaga cttccaacga gatgcgatga atcttcgcgc cacctgctgt
     1861 ttcaagggtg gcattagatc gctgttcact catttgagca acccattcag gagtaggtcc
     1921 gaatactgat cggcgatttg ttcaatggat aattcgccat ccgggcgata ccaggtgatc
     1981 gtccagttca tgatgccgag taaggcgcgt gtagcgaggg cggggttatc acacttgaag
     2041 agcttcgact tcaccccttc tgcaatcaca tctttccaaa gcgactcgaa cttgtctcga
     2101 tttggaatgt ggcgcgcgtg ttgtctccgc tccagcgcac gatgttcaaa tagcaggacg
     2161 gtcgccaggt caatgttttc gataaggatc tggagatagg ctcggaccat cagacgcagt
     2221 tttttatctg cgaggatatt ttggtttgaa atggatgaaa tctgttcgag gagcagttgt
     2281 aaggcacgat ccaaaatatc gagcaggatc tcctgtttgg aggaaacatg gtgatacaga
     2341 ctcgcctttt gcaggtgtac ggcttcagca atatcgttca tcgacgcgcc atgaaacccc
     2401 ttttggcgaa acacttgcgc tgcagcttcg agaatatctt ctctcgtcat aaatgctccc
     2461 taccgaacgg tcggttgttg tgatataata ctggatgcac cagtgcatgt caaggttggt
     2521 acaatagaaa taggttacag tcgaggagaa aaacaatgaa atattttgtg accggtgcaa
     2581 ctgggtttgt cggcggagtt ctggccaaga aattgcgcga agcgggacat gaggttaatg
     2641 cttctgtccg tagcccaggt aagacaaaag agttacagga ccttggagta aagatattca
     2701 aaggtgatgt gaccgataaa gagtccatgc gcgaagccat gactggtgtg gatggggtct
     2761 ttcatgtggc aggctggtac aagatcggcg tcaaggataa gagtgacggg gaaagagtca
     2821 acattcaggg aacgcgcgct gtgctggaat taatgcagga gttaagaatc ccaaaaggcg
     2881 tgtataccag ttccctggtg gttaattctg atacccaggg tgaattgaag gacgaaagtt
     2941 atcatttcac cgggcagcac ctcagtgaat attcccgcac aaaagcagtc gcccatgaga
     3001 tggcaaagga atttatcgca aagggacttc cccttgtcat tgtgatgccc ggtttgattt
     3061 acggacccgg ggatacatcc atcctgcgcg ctaacattcg agacttccta aaccaaaaac
     3121 tgccgctgct cccctctcag aacgcgtatt gctgggcaca cgtggatgac atcgtacagg
     3181 gccacattct ggctatggaa aagggaagaa tcggcgaaag ctatatcatc gccggggaac
     3241 ctgtcacaac agcggatgca ttcaaactgg caggcgagat tacgggcaaa cgcgtgccaa
     3301 gtgtggtttc atacaatttg attaaggcgt tgtccgtcat tgtcaggccc ttcgataatt
     3361 tcctccccga aaccttcacg tcggaaggat tgcgcatgat tgctggcgtc accaattggg
     3421 gcgacaacag caaagccaaa cgtgagctgg gctatcaccc gcgacccttc cgcgagggat
     3481 ggggagaaac cctactccac gagcaaaaat tgttgaaaca atcctaaaga tgctataata
     3541 tttttatgtc cacttctgaa ttcgaacagc ctgttccacc acttgtgtct gaacctgtga
     3601 cgtcaccaga cgatcaagac aaacggatga gacgcatcct gattggtgtc attgtgggtg
     3661 cggtgattct tgtgatcctg ctcggtgtgt cgatctacct cctgcttcaa ccggctacac
     3721 ccaccgatcg aattcgcgat gtgtttatta tagttgtggc gttggagtca ttggtaatcg
     3781 gtgtggcgat gatcgtgctg attatgcagc ttgccagctt aatcaacttg ctgcagaatg
     3841 aaattcgccc cattttgcac gcgaccaatg agacggtaaa tacgttaaga ggtacagcag
     3901 agtttttagg cgacaatgtc gtagaaccag tcatcaaact taatggctat ctggctggtt
     3961 tatatcgtat gttagaattg atgggcattc gtaagaaata aattccagaa aacacgcgct
     4021 cagcaggtcg catggctgag aatcgctgct ggagcaatat aagttaataa tcaaaaggag
     4081 attactatgt ctgaacgtga tgaatttggc gcatttctgg taggttttat tgtcggcggg
     4141 ctgaccggct ctgtcgttgc actgcttttc gctccccaat ctggagagga aacacgcgca
     4201 gtaattcggg ataaatccat tgagctgcgc gataaggcac agatttcggc agaagaggca
     4261 attgcccgcg ccgaagccgc tgcggctgaa gcccgcgccc gtgccgatga acttgccaag
     4321 caactccgtg aacgcggcac tgaagtttac ggcaccgttc gcgatcgcgg ccaggcggtt
     4381 tacgaagacg tccgtgagcg cggcaaagct gcttacgacg atgttcgtga tcgcagcaag
     4441 agcgccgtgg ataatatccg cagcaaggtg aagaaggaag aaggcgaaac cgccgccgga
     4501 taaggcaaat cgcaacatgt tgagggtttg gctgctttcg caaagcggtc aaacccttgt
     4561 ttttttaatt gtgccatcgc tgctaacatg aaagcatttc ttagatactt tttcaaactg
     4621 ctctaccacc agtttgcctt cgcttatgat ctggttgccg ccagtgtttc atttaatcgg
     4681 tggaaagagt gggtaaaaag cgtcatcccg cttatcgacg gaaatcggat tttggagatc
     4741 ggtcacggac ccggtcattt gcaccgggct cttctggacc gcggcttgtt cgccgttgga
     4801 atagatgaat caaagcagat gggtcgtctc gcgaaacgcc gcgccggaag cactgcccgt
     4861 ctcacgcgcg gactggcaca acatctcccc ttccccagtg aatcattcga taccgttatt
     4921 tcaacatttc ccgcagagta catcaatgac cctcgtaccc tggctgaggt gaagcgatgc
     4981 ctgagaagca gcggtagatt tatcatcctg ccggttgcat ggccaaaaaa tcctttctta
     5041 gagtggttat tccgcgtcac aggtgaatca ccatccgaag cgctggaact ggtgaaggca
     5101 aaattcaggg aaccatttgc aaaagcaggc ttcagtacca aagtggaaac ccttgatgta
     5161 aaatcgagca tcttgttaat tattgtggca acaaaactgt cgccgtgatc gaacgtacaa
     5221 aggatggttt atgttcaaaa aattacgcga acccgtcaat agcctcacac attgggccgg
     5281 agctttcctg gcactttgtg gcttgatagc gctccttgtg gtcggctggg gcacacctgc
     5341 aaaggtgatc tcactgttaa tttatggggt aagcctgatc tttttatttt ctgccagcgc
     5401 cacgtatcac atggtgcaag taaaagacaa ggcgttggaa atcttccgca agattgacca
     5461 tgccgcgatt tattttctca tcgcagggac ctataccccg ttttgcgtca acgcatttag
     5521 cgggttttgg caatggggga tgctgagtat catctggtct ctggcgttga ttgggatcac
     5581 gataaaagtg ttttatatcc gcgcgccgcg ctggttgaac gcagggatat atcttgtgat
     5641 gggctggcta tgtcttgcgg ccatagggca gatgttaatc actctgccgg cttgggtcat
     5701 tggctggctc gtcgcgggcg gtattatgta cacgcttggc gcagtcgtat acatcacaaa
     5761 gatattcaac tttgtccctg gcatatttgg ttttcatgaa atatggcata tctttgtgat
     5821 gcttgccgcg gctgcccact ttgtcgccgt actgggagtg gcgctttaga attcgatccg
     5881 atttgctcgc gggatacatg cgaaacacaa gactctacac ggcagaaaat atgggatagg
     5941 agagcaaaat gttatttgga tttgatctca atcaattatt attattcccc atcaaagatg
     6001 acgaggcacg aaaacacttt atagtgggat gtttgattta tttagcaggt ttttttattc
     6061 caattctgcc ctggctggta acgtcgggct atagtgccat tattattcgc caggtcttga
     6121 atggagaaaa gccacacatg gtgccatggg ataattggga gcaattattc aaagatggtg
     6181 cccggctttt tggcataacg ctgatctact cccttccgct ttttgtactt atgatatttt
     6241 tcttcttatc attctttgca gttccacttc ttccagcgtt aacgaagggt gacaacaatc
     6301 aagggcttgg aatcggatgg ttcatattca tgttgatcgg aatgggagtt tcattcctcg
     6361 tctttcccct ttccctggcg atcggattga tcgttcctgc agcagagatt catgcagtcg
     6421 cgaaagatga ctttatggct gggtttcgcg ttcgggaatg gtggccgatc ttcaggcaga
     6481 actggcaagg attcgtcgta gcaatggcca tcctatatgc tttactgaca gcaatgtcgt
     6541 ttgcgatgca attcctgatt attactgtag ttttgttttg cttattgccc ttactaatac
     6601 cagtgatatc catgtattct gcaattattc aatttgcgac atttgcacag gcatataagg
     6661 aaggcaggga caagttgagt gtagtaagtg cgcaagcgcc taccacaggc gagaccgact
     6721 tgcaaatcca aacgtcatag caatctcaag gctgagtcgt cggtttttac cctcttcgca
     6781 gtaata
//
LOCUS       DASTJP010000868         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_553916, whole genome shotgun sequence.
ACCESSION   DASTJP010000868 DASTJP010000000
VERSION     DASTJP010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553916"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1656
                     /locus_tag="VFQ23_25160"
     CDS             <1..1656
                     /locus_tag="VFQ23_25160"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HET9909962.1"
                     /translation="VAEISVFRDIRDQGSLEPLTQNIGDILKFFHQTGDLQGKHNLPG
                     EPMTDPITGLLFAIGVAYAIVGGRDQRYFLLLIWLVIGLAGSFLSSNHESPQSYRALT
                     ALPAVVFLAADVLDRIARAVYRGLQEYSVSTSIPNFSSIAVGGVIVLALTGSALWESN
                     VYFGRQADSIDVVRGFNPTENSVAQETMAALQAGDEVYLSPGFSSYSPLRFLVYGVIK
                     EKTGQNTLEDRPYRVALPEVNLPFPDNGHNVLMLLDSEYWSLRDYITSFYPQANMELI
                     RLADDSPIYFRVEIPHAQIAELQGITQHITYLDGRSEIGSASQIEISDPQIREALWEG
                     AIRLEHGGQYELRGENGLQVFLDDQLFEGKHYLGRGLYRLHLSWKSGSGNNPRLLWQV
                     NDQAPEPVPPEALFRITGASHGLLGTYWSNRNWEGDPVFHQVTPFLLLAWPDEQPIVP
                     NGEFSARYTGTLRVTDPGSYLLRIKADDGARLILDGDVLGEGLIAGQPNDIEAITELE
                     ARDYPIQIDYFQQGGGSGLRLFWSYNGGPLTPIPPTALFPAKP"
     gene            1666..>2722
                     /locus_tag="VFQ23_25165"
     CDS             1666..>2722
                     /locus_tag="VFQ23_25165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9909963.1"
                     /translation="MSSTDVNMPPPSSNALKGWGQNLNLKQIVNLFDRPYASTIAMVI
                     ILLVAALFRFHGLNWDEGRHLHPDERFLSTVTNDLQWPESLENYFDPTTSTLSPYSLP
                     NMGLYVYGTLPVYIVKWTAILLDRNNYDKITLIGRNLSGLFDVATIFILFLIGKRLYG
                     KKIGLLAAFLMALSVLNIQLSHFYTVDTFANLFVVGTIYAILRANESGRWLDHALTGL
                     LFGLGLASKLNVILLIVPIVIGAGLNLYQHARDGNLRAALEHTLVRLFSVLFIAALTF
                     RVVQPIAFSGPSFWDWSLNPRWVEDIGEQQKTLAGDSDLPWIQQWTNRSIMFPLKNMV
                     LWGMGIPLGLASLAGVGL"
BASE COUNT          645 a          637 c          674 g          766 t
ORIGIN      
        1 gttgcagaaa tcagcgtttt tcgtgatata cgcgaccagg gcagcctgga accactgact
       61 caaaatattg gtgacatcct taaattcttt catcagacag gtgaccttca gggaaaacac
      121 aatctgcctg gcgagccgat gacggatccg atcaccggat tgttgttcgc tattggagtg
      181 gcatacgcaa ttgtcggcgg gcgcgatcag agatatttcc tcttattgat ttggctggta
      241 attgggctgg ctggaagttt tttgagttcg aatcacgagt ctccacaatc ctatcgcgca
      301 ttgacagcct tgcctgcagt agtattcctg gcggcagatg tgctcgatcg tattgcacgt
      361 gcagtctatc gaggcctgca ggagtattct gtttcaacct caattccaaa tttttcatcc
      421 atagccgtgg gtggggttat tgtcttagca ttgactggct ccgcgctttg ggaatcaaac
      481 gtttacttcg gacgtcaggc agattcgata gatgtcgtac gtggttttaa tccgacggaa
      541 aatagcgttg cgcaggagac aatggcagcc ttgcaggcag gcgatgaagt ttatctttct
      601 cctggcttct cctcgtattc gccgttgcgg tttctggtct atggtgtgat aaaagagaag
      661 accggacaaa acacgcttga ggacagacct taccgtgtcg ctttgccaga agtgaatctg
      721 ccatttcctg ataatggaca taacgtcttg atgctcctcg atagcgagta ctggtctctc
      781 cgcgattaca tcacatcttt ttatccccag gcaaatatgg agttgattcg cctggcagat
      841 gattccccca tttatttccg tgtggaaatt ccacatgcac aaattgccga gcttcaagga
      901 atcactcagc atataactta tctcgatggt cgaagtgaaa taggctctgc ttcgcaaatt
      961 gaaataagtg atccacaaat cagggaagct ttgtgggagg gagcaatccg cctggagcac
     1021 ggcggccaat atgaattgcg cggtgagaat ggacttcaag tttttctcga cgatcaatta
     1081 tttgaaggca aacattattt gggacgcggg ttataccggt tgcatctgag ctggaaaagt
     1141 gggtcgggaa acaacccgcg cttgctctgg caggtgaatg atcaagctcc tgaacccgta
     1201 cctccagagg cactctttcg tattaccggt gcttcgcacg gactactcgg aacatattgg
     1261 tccaatagaa actgggaagg cgaccctgtt ttccatcagg tgaccccgtt tcttttgctt
     1321 gcctggcccg atgaacaacc tatcgttcca aacggagagt tcagtgcccg ctacactgga
     1381 accctgcggg tgactgatcc tgggagttat ttgttgcgca tcaaagccga cgatggagca
     1441 cgtttgatcc tcgatgggga tgtacttggc gagggattga tagccggtca accaaatgat
     1501 atcgaggcaa ttactgaact tgaggcacgc gattatccaa ttcaaattga ttatttccaa
     1561 caaggtggcg gcagcggtct gagactattt tggagttata acggcggacc gctaacccca
     1621 attccgccga ccgctctttt cccagccaaa ccgtaggagt acctcatgag ttctaccgac
     1681 gttaacatgc cacctccttc ttcgaatgct ctaaaaggtt ggggccagaa tctcaatcta
     1741 aaacaaatcg ttaatttatt tgaccggccg tatgcctcca cgattgcgat ggtcattatc
     1801 ttattggttg cggcattatt tcgctttcat ggtctaaact gggatgaagg ccgacattta
     1861 caccctgacg aacgttttct atctactgtg accaatgatc ttcagtggcc agagagtctc
     1921 gaaaattatt tcgatcccac cacctcaacg ctcagtccat actcattgcc caatatggga
     1981 ttgtatgttt atgggaccct gccggtctat atcgttaaat ggactgcaat tctgcttgac
     2041 cgaaacaact atgacaagat cacgctcatt ggtagaaacc tctctggact gtttgatgtc
     2101 gctacgattt tcattctgtt cctgattggg aaacgactgt atggcaaaaa aattggtttg
     2161 ctggctgcct tccttatggc gctcagcgtg cttaacatac aactttcaca tttttatacc
     2221 gttgacacct ttgcgaatct ttttgtggtg gggacgattt acgccatctt gcgagccaat
     2281 gaaagcggcc gttggcttga tcatgcgttg accggtctac tgtttggact tggacttgca
     2341 tccaaactta atgtcattct gctcattgtg ccaattgtga ttggagcagg tctcaatctg
     2401 tatcagcatg cgcgtgacgg aaatctacgg gctgcacttg aacataccct cgttcgttta
     2461 ttcagcgtcc ttttcatcgc cgcgttgacg tttcgggtgg tacagccaat tgcttttagt
     2521 gggccaagct tttgggattg gtcactcaat ccgcgctggg tagaggacat cggggaacaa
     2581 cagaaaacgc ttgccggaga ttctgatttg ccgtggattc agcaatggac caatcggtcc
     2641 atcatgtttc cattaaaaaa tatggttcta tggggaatgg gaataccgtt agggctggca
     2701 agcctggcag gggtagggtt gg
//
LOCUS       DASTJP010000869         8553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_716960, whole genome shotgun sequence.
ACCESSION   DASTJP010000869 DASTJP010000000
VERSION     DASTJP010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716960"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..6023
                     /locus_tag="VFQ23_25170"
     CDS             153..6023
                     /locus_tag="VFQ23_25170"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET9909964.1"
                     /translation="MLICWAVSPYMAARISRPAVQPDKRLSSTQENKLRILARSTWLY
                     FEHFVGPEDRWLPPDHFQESPRGQPSHNTSPTNIGLMLLSTLSAHDMGYIGPLEFSLR
                     IRDAFDSMDSLERVRGHFLNWYDTRTLAPLPPRYISTVDSGNLAASLIVLREGCLQLP
                     RSSVLQLGGLLDTLNLLAYIIEQAELDEAANELREAIDALRAQVETLNDPGQFSTEAM
                     MRLLQDSRIEMENMLWEAVQNSEEEIAPDVLRSLSIWINRVRYQLKHIRTDLQVLAPW
                     LLALPNKPHPESQTDEKSELATAWADLETSLTLHPKLGEIPEICIRSRSILEQITNNL
                     GENDQLALDWCKSLAYDLDSAQKNASSLLADYSALASRAEAFFQAMSFSFLYNSQRRV
                     FHIGYNVESGRLDSNYYDLLASEARIASLIAIARGDISQNHWLYLARPLTQVNDTRAL
                     LSWSGTMFEYLMPNLFAESYPNTLLDQSSEAAVEEQMRYAAEKGIPWGISEASYYGFD
                     AAQIYQYRAFGVPTLGYKRGLAEDLVIAPYASILALPIMPNAVMQNLEWLEQLKMWGL
                     YGLYESVDFTPERLKTGEEHAIVRSYMVHHQGMILLTLSNYLFNKRMIRRFHADLRIQ
                     SIELLLQEQIPADAPTEHPRPQPIDSMRASYTAIPLDPWRVSPEAPYTQVHSLSNGKY
                     SLLITAAGSGFSRWGNIELTRWRADSTLDNWGSWIYVEDRHTRKVWSATRQPSMIPPG
                     GSDVRFFPHRAEFERQDEDLILNTTVSVAPNDNVEIRRMTLVNKGNRSRVIALTSYAE
                     IILADQSVDRRHPAFNKLFIESEFLPNEEVLLFRRRPRSADEKPIYLAHFFISNASDT
                     HLTGYETDRERFLGRGGTPEMPKVFSSSSDASRLSGTTGATLDPICALQAEINLSAFE
                     STQVAFITLAAGSRKEALDLARRYRRWTNLRRAIDEARTHSEKEFSQLELTSRKLEQI
                     QKLLSPLLYPTSALRAEPDVLATNTLGQPGLWTFAISGDYPILLVRLRNEENLGLLRE
                     VLLAHLYWRKRGLMIDLVIFNQHETSYQQDFQSKVGRLLARTGGDAWMNKRGGIFIVN
                     EDQMNEAERILLRTAARVILDGESGSLEQQLSRLDSAPVRLPRFVPITSMKSPEDVNR
                     VQRPKDLLFDNGTGGFSPDGHEYVIYLERDQWTPAPWANVIATPEFGCLVSESGMGAT
                     WSKNSGENRLTPWRNDAVSDTPSEAIYLRDEDSGQIWSPTPLPARDDAPYLIRHGAGY
                     TIYEHASHGLEQNLRVFVVPDEPLKIVQLKLHNATKRMRRINATYYAEWVLGTTQENT
                     AAYIVPEFISGHFALLARNTYNMDFGQRVAFLASTREPHGITTDRTEFIGKHGSYDRP
                     AALERVGLTPNIHAGGDPCAALQTLLWLQPGETKEVTFLLGQGADRADTERLISHYKN
                     IHHVEAAWDSVGKFWDDILEQTQVDTPEPGMNLLLNRWLLYQALSCRIWGRTAFYQSS
                     GAYGFRDQLQDVMGLIHVRPDLVRQHILEAASHQFEEGDVLHWWHPPSGRGIRTRCSD
                     NLIWLPYVTAYYIQATGDQSILTQEVPFLRAEPLKPKEHERYGEFPAGEMGTLYEHCC
                     RAIAKGTTAGPHGIPLMGAHDWNDGMNRVGAEGQGESVWLGWFLSTTLTNFADVCESM
                     EDGARAETYRAQAKEIIKAIELSSWDGAWYRRAYYDDGSPLGSAANNECKIDSIAQSW
                     AVISKGADPFHAQMAMDSVYEKLVRFDEQQILLFTPPFDRTARDPGYIKGYPPGIREN
                     GGQYTHAALWAIWAFAEMGEGDRAGQLFNLINPIHHADTPEKSSRYRVEPYVIAADVY
                     SVPPHAGRGGWTWYTGSAAWMYRLGTQALLGLNRIGDTLQVKPSIPSGWTEYQLNYRF
                     GKTMYHILVRNKAGTHHKGNQILLDGELLKDANIPLVDDERNHEVMITLAARKSTK"
     gene            6234..6851
                     /locus_tag="VFQ23_25175"
     CDS             6234..6851
                     /locus_tag="VFQ23_25175"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HET9909965.1"
                     /translation="MKSGSKNSSSTPPPSKMKERRLARQREKQRAQLIRIGSIVGGIV
                     LVFLLIYFIGNSNQPNVDIEGLQTFNISGGHTTEPVTYPEVPPVGGVHNPAWQTCGVY
                     SQPVANENAVHSMEHGAVWITYQPDLPAAEVEALQTLTRQSGHRLLSPYPDLPSPIVI
                     SGWGNQLQLEKADDPRLSQFITAFEQSPRVPEPGASCSGGVGEPE"
     gene            6858..7532
                     /locus_tag="VFQ23_25180"
     CDS             6858..7532
                     /locus_tag="VFQ23_25180"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HET9909966.1"
                     /translation="MNPSNQTAVDESAPSSASRWFDPPVLWVFVSLVLGVVLGFWGGQ
                     QSMRPPTEDSAEVGFARDMANHHAQAVTMAQLVYDRTDDENMRLLALDIMLTQQAQIG
                     QMQGWLTTWERPLSTTTPAMTWMGMPTSALMPGMATAEEINRLRGLKGTEADGLFLQL
                     MIPHHRGGVEMANAVLEYTQRAQVVAMAQAIVNSQTTEIAYMQQLLHDKGFPPVPEET
                     SPHHAP"
     gene            complement(7545..7760)
                     /locus_tag="VFQ23_25185"
     CDS             complement(7545..7760)
                     /locus_tag="VFQ23_25185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909967.1"
                     /translation="MDKCLYGNTFFTQRVTKVVVTICDHEGNTIEQGQATLIADTLWK
                     YATSAPAEATLIVDAVDLAGNITKYGA"
BASE COUNT         2071 a         2213 c         2228 g         2041 t
ORIGIN      
        1 gggtgaccgc tgcccataca gttaaattat ttggcaggcg gttgaaagtt cgagccgcct
       61 ggcaggcaat gattgttgcg cccgcctttg caattgcttt gtttctcctc atattgtccc
      121 tatacccaca ggagttgctg gaggctgctc cgttgctgat ctgttgggct gtatcgcctt
      181 acatggctgc gcgcattagt cgaccggcag ttcaaccgga caaaagactt tcaagcacac
      241 aggagaacaa actccgaatt ttggcgcgct ccacgtggct ttattttgaa cactttgtag
      301 gtcccgaaga tcgctggctt ccacccgatc acttccagga aagtccacgc ggtcaaccct
      361 cgcataacac ctcaccgacc aatatcggct tgatgttact ttccacactt tctgcgcatg
      421 acatgggcta catcgggccg ctggaatttt cgctgcgcat tcgcgatgct ttcgacagca
      481 tggattcact cgagcgagtc cgaggtcatt tcctgaattg gtacgatacg cgcacgctgg
      541 ctcctctgcc tccgcgatac atctctacag ttgacagtgg gaatttggca gcgtccttga
      601 tcgttttgcg agaaggctgt cttcaattac cccgaagctc cgtgctgcaa ctgggaggct
      661 tgctggacac attgaacctg ctcgcctaca taatcgagca agccgagctg gatgaggcgg
      721 ccaacgagct gcgagaagcg atcgacgctt taagggcaca ggtagagacg ttgaacgatc
      781 ccggtcaatt ctctacggaa gcgatgatga ggttattgca ggatagccgt attgaaatgg
      841 aaaatatgtt gtgggaagct gtccaaaact ctgaagagga aatagcccca gacgttctgc
      901 ggagtctttc tatctggatc aaccgtgtac gctaccagtt gaaacatatt cgaaccgatc
      961 tgcaggtttt agcgccctgg ctgttggcgc taccgaataa gcctcaccct gaaagccaga
     1021 cggatgaaaa atctgagctc gccactgcat gggcagattt agaaacgagc cttacgttgc
     1081 atccgaaact cggagaaatc ccggagatct gtatacgctc gaggtcaatt ctggagcaga
     1141 tcaccaacaa tctaggtgaa aacgatcagc tcgctctcga ctggtgcaag tcactcgctt
     1201 atgatctcga ctcggcgcag aagaacgcct cctcgctcct ggctgattac tcggcactcg
     1261 cctcaagagc ggaggcattc tttcaggcaa tgagcttcag cttcctgtat aactcgcagc
     1321 gccgggtgtt tcacatcggc tacaacgttg aatcgggccg attggattcc aactactacg
     1381 acctgctggc ttctgaggcg cgcatcgcca gcctgatcgc gatcgcgcgt ggtgatattt
     1441 cacagaatca ttggctgtat ctggcacgtc ccctaactca ggtgaatgat acgcgagcgc
     1501 tcctctcctg gagcggcacc atgttcgagt acctgatgcc caacttattt gcagagagtt
     1561 acccgaacac tctcttggac caaagctctg aggcggctgt agaggagcag atgcgctatg
     1621 ctgctgaaaa aggaatcccg tggggtattt cagaagccag ctactatggt tttgatgctg
     1681 ctcaaattta ccagtatcgc gccttcggtg tccctacgct cggctataag cgcgggctgg
     1741 cagaggattt ggtgatcgca ccgtatgctt caatcctggc gctgcccatc atgcccaatg
     1801 cagtcatgca aaatctggaa tggttggagc agctcaagat gtgggggtta tacggcttgt
     1861 atgaatcagt cgatttcaca cctgaacgcc tgaagaccgg tgaagaacac gccattgttc
     1921 gttcgtatat ggttcatcac cagggaatga tcctgctgac actctccaat tatttgttca
     1981 acaaacggat gattcgacgg ttccatgctg acctgcgtat tcagagcata gagctattat
     2041 tgcaggaaca aataccagca gatgcaccca cagagcaccc acggccacaa ccaatcgact
     2101 cgatgcgcgc ctcatacacg gccattccac tcgacccctg gcgagtttct cctgaggcac
     2161 catacacaca ggtgcattct ctttccaatg gaaagtacag cttgttaatc actgcagccg
     2221 gttcgggctt cagccgctgg ggcaatatcg agctcacgcg ctggcgagcc gactccacac
     2281 ttgataactg gggaagttgg atctatgtgg aagaccgcca cacccgaaag gtatggtcgg
     2341 caacccgtca gccatccatg atcccaccgg gtggaagcga tgtgcggttc ttccctcacc
     2401 gcgccgaatt tgaacgacaa gatgaagatt tgatcctgaa taccaccgta agcgttgcac
     2461 ccaatgacaa cgtggaaatt cggcgaatga ctctcgttaa taaagggaat cgttcgcgtg
     2521 ttattgcgct cacgagttat gcagagatca tcctggcaga ccaatccgtg gaccggcgcc
     2581 atcctgcatt taacaaactt tttattgaaa gtgaatttct gcccaatgag gaagtgctgc
     2641 tcttccgtcg ccgcccgcgt tcggcagatg agaaaccaat ttatctggca cattttttca
     2701 tcagcaatgc cagcgacact cacctgacag gttatgaaac agatcgtgaa cgattccttg
     2761 gacgcggtgg cacacctgaa atgccaaaag ttttttcaag ttccagtgac gcatcccggc
     2821 tttcgggcac gacgggagcc accctggatc cgatttgtgc actgcaagcc gaaattaatt
     2881 tgtcagcttt tgagtcaaca caggtagcat ttatcacact ggcggctggc tcacgcaagg
     2941 aagcacttga cctggcgcgc cgttatcgtc gctggaccaa tctcagaagg gcgattgatg
     3001 aggcgcgaac gcacagcgaa aaggaatttt cacaactcga acttacatcc cggaagctgg
     3061 agcagattca aaaactgctt tcgcctctgt tgtatcctac ttctgccttg cgagccgaac
     3121 cagatgtgct tgctacgaat acattgggac agccaggctt gtggaccttc gcgatctcgg
     3181 gtgattaccc aatattatta gtgcgcctgc ggaacgaaga aaatctcggc ttattgaggg
     3241 aagtgctcct ggcgcacctg tattggcgta aacgcggctt gatgatcgac cttgttatct
     3301 tcaaccagca tgagaccagt taccagcagg actttcagtc aaaggttggg cggctcctgg
     3361 cacgcaccgg cggtgacgcc tggatgaata aacgcggtgg aatctttatt gtcaatgaag
     3421 atcagatgaa tgaagcagaa cgtattcttt tgcgcactgc tgcgcgcgtc attctggatg
     3481 gagagtctgg ctcgctcgag cagcagttat ccaggctgga ttctgccccg gtccgcctcc
     3541 cgcgctttgt tccgattacc tctatgaagt cgcctgagga tgtcaacaga gtgcaacgcc
     3601 ccaaagattt gttatttgat aatggaacag gcggttttag tcctgatggg catgagtatg
     3661 tcatctacct tgaacgggat caatggactc ctgctccgtg ggccaacgta atcgctacgc
     3721 ctgaattcgg atgtttggtt tctgaatcgg ggatgggcgc tacctggtca aaaaacagcg
     3781 gtgagaatcg actgactccg tggcgcaatg acgcagtgag cgatacgcct tcagaagcaa
     3841 tttatttgag ggatgaagat tcaggtcaga tctggtctcc gacgcctctg cccgcgcgcg
     3901 acgatgcccc ttatctgatt cgccacggcg caggttatac gatctatgaa cacgcaagtc
     3961 acgggctgga acagaacctg cgtgtgttcg ttgtccccga tgaaccgctc aaaattgttc
     4021 aattaaagct tcataatgca actaaacgaa tgcgccgcat caacgccacc tattatgccg
     4081 aatgggtgct tggcacaacc caggaaaata cagccgcata tattgttccc gagttcatct
     4141 cgggtcattt cgctttgctg gcgcgcaaca cctacaatat ggactttgga caacgcgtgg
     4201 ctttcctggc atccacacgg gagccacatg ggataacaac cgatcgcact gaatttatcg
     4261 gaaagcatgg cagctatgac cgtcctgcag cgctggagag agtcggtctg acgccaaaca
     4321 tacatgccgg cggtgaccca tgcgcagcat tacagaccct gctctggctg caacccggcg
     4381 aaacgaaaga agtcacattc ctgttagggc aaggggcaga ccgggcggat accgagcggt
     4441 tgatcagcca ctataaaaac attcatcacg ttgaagcggc atgggactct gtgggtaaat
     4501 tctgggatga tattctggag caaacacagg tcgatacccc tgaaccggga atgaatcttc
     4561 tcttgaaccg ttggctgctc tatcaggcac tttcctgtcg aatctggggg cggacggctt
     4621 tttatcaatc cagtggcgcc tacggcttcc gcgatcaatt acaggatgtg atgggcctga
     4681 ttcacgtgcg acctgacctg gtccgccagc acattctgga agcagccagt caccaatttg
     4741 aagaaggtga tgtgcttcat tggtggcatc caccttcggg gcgcggcatt cgaacacgct
     4801 gctcagataa cctgatctgg cttccttacg tcacagccta ctatatacag gccaccggag
     4861 accaatctat tctcacgcag gaggttcctt tcctgcgggc tgagccgtta aaacctaaag
     4921 agcatgaacg ctatggggag ttcccagccg gggagatggg gacactctat gagcattgct
     4981 gccgcgccat tgccaagggc acaacagccg gtccgcatgg gattcctcta atgggcgctc
     5041 acgattggaa cgatgggatg aatcgcgtcg gcgccgaggg tcagggtgaa agtgtatggc
     5101 tcggatggtt cctatccacc acgctgacga attttgcaga cgtttgtgaa agcatggagg
     5161 atggcgcgcg tgcggaaacc taccgtgcac aggcgaagga aataatcaaa gccatcgagt
     5221 tgagcagttg ggatggggcc tggtatcgcc gagcatatta tgatgatggc tcaccgcttg
     5281 ggtccgcggc aaataatgaa tgcaagatcg actcgattgc gcaatcgtgg gcagttatct
     5341 ccaaaggtgc ggatcccttt catgcgcaga tggcaatgga ctcggtctat gagaaactgg
     5401 ttcgattcga tgaacagcag attctactat ttacacctcc cttcgatcgg actgcgcgtg
     5461 atcccggtta tatcaaggga tatccccctg gcattcgtga aaacggaggg cagtatacgc
     5521 atgccgcact ctgggcaatc tgggcgtttg ctgaaatggg cgaaggcgat cgcgctggtc
     5581 aattgttcaa cctcattaac cccattcatc atgcggatac acccgaaaag agtagccgtt
     5641 atcgcgtgga accgtatgtg attgcagccg atgtgtatag tgtgccacct catgccgggc
     5701 gcggcggctg gacctggtac accggctctg ccgcctggat gtaccggctc ggtacgcagg
     5761 cattgctcgg tttgaatcgc atcggcgaca cgttacaggt caagccgagc attcccagtg
     5821 gctggactga atatcaacta aattatcgtt ttggtaaaac gatgtatcac atactggtca
     5881 ggaataaggc aggcactcat cacaaaggca atcagatcct tttggatggc gagttgttga
     5941 aggatgcgaa catcccgctc gtggatgacg aaagaaacca tgaggttatg atcacgcttg
     6001 ccgcccgcaa atctacaaaa tgatcgcgaa cgaaggataa gttactaagt cagggagtgt
     6061 ccaagtgtat gggcttagcc acagagtgct ttgagttttt ttctcattga actcggtaat
     6121 ctctgtggcg aaagtataga gttcgcctat ccttttattt ctttaatcca cgcaaccgac
     6181 tcgactcatc agaatcagat aaaatcatca aaatactcta ctcgaggtta ttcatgaaat
     6241 ccggatccaa aaattcttcc tccactccac ctccctctaa gatgaaggaa cgacggcttg
     6301 cccgtcagcg tgaaaagcaa agggctcagc tcatacgaat cggtagtatc gtgggtggca
     6361 ttgtgcttgt gttcctgctg atttacttca ttggaaacag caatcagccc aacgttgaca
     6421 ttgagggact gcaaacattc aatataagcg gcggtcacac aaccgaacct gtcacctatc
     6481 cagaggttcc gcctgttggt ggcgtgcata accctgcctg gcagacctgc ggtgtatatt
     6541 ctcagccggt tgcgaacgaa aacgcagtcc attccatgga acatggcgcg gtctggatca
     6601 cgtaccagcc tgacctgccc gctgccgagg tggaagcttt acagacactt acgcggcaga
     6661 gtggccacag actgctaagt ccgtatcctg acctgccaag cccgatcgta atctcaggat
     6721 ggggcaatca gctccagttg gagaaggcgg acgacccgcg cctttcacaa ttcatcaccg
     6781 ccttcgaaca gagccctcgc gtgcctgaac caggtgcatc ctgctccgga ggcgttgggg
     6841 agccggaata atcaacgatg aatcccagca accaaacagc tgtggatgag agcgcaccat
     6901 catcggcttc ccgctggttt gatcctcccg ttctttgggt attcgtctcc ctcgttctgg
     6961 gagttgtgct cggcttctgg ggtgggcagc aatccatgcg cccacccaca gaagattcgg
     7021 ctgaggtcgg ctttgcccgc gatatggcaa accaccatgc tcaggccgtg actatggcgc
     7081 aattagtcta tgaccggacc gatgatgaga atatgcgttt gctggcgctg gatatcatgc
     7141 tcacccaaca ggcacaaatc ggtcagatgc aaggctggtt gaccacctgg gaacgtccgc
     7201 tttcaaccac gactccggct atgacgtgga tgggaatgcc gacctcagca ctcatgccgg
     7261 gcatggcaac ggcggaggaa atcaaccgtc tgcgcggctt gaagggtact gaagccgatg
     7321 gcctgttttt acagttaatg attcctcatc atcgaggtgg tgtggaaatg gcgaacgctg
     7381 ttctggaata cacgcagcgg gcacaagtcg ttgcaatggc tcaagccatt gtcaattcac
     7441 aaactactga gatcgcttat atgcagcagt tgcttcatga caaaggcttt ccacctgttc
     7501 cagaagaaac aagcccgcat catgccccat aaactcgtag agagttaggc tccatacttc
     7561 gttatattac cagccagatc aactgcatcc actatgaggg tggcttccgc aggggcggag
     7621 gtggcatact tccataacgt atcggctatc agcgtggctt gtccctgctc tatagtattg
     7681 ccctcgtgat cacaaatggt gaccacgact ttcgttaccc gctgtgtgaa gaatgtgttg
     7741 ccgtatagac acttgtccat aaatttcttg gggaatttcc ctacttacgc cgcgaggggt
     7801 ttaaaagtaa aacagagcga atagcaagag aagaaaggca agtgcgtgtt gtgattagtc
     7861 agagaaagct tatcgtgact gtttattatg tttattgtga tcactgtgga gcacggggac
     7921 cagacgggat caacgaggta gatgcacata agattgcaaa gcagcgggga tgggaaatca
     7981 tcgaggacac atatgtttgc cctgatcctc gtcatgccga actcaaaaag atgcgcgagc
     8041 ttctgctaga aattaaagcc gaacccgacc cgataccctt agtgaattgg tggagatagg
     8101 ttccctatgt tcactcttga aactggtact gcctatttag ccgcaatttt gcttgttgca
     8161 atattacttg ccttgtttcc atctgaggaa agatacaaat agctggggac tggcacttgc
     8221 agaagtgacg ttatcacttt gctgttgtgg tttagctgaa agatgtaatt cgcgtgattt
     8281 cccctccgtg gtatgcagcg cctctccttg agcgggcagc ttcgggaaag tacgaattta
     8341 taggtccgac tcttgaactg gacttgtacc tataaatcaa acagcctcag tgagacgatg
     8401 tggggcgaaa gaaagaaccc gtccgctaat tttcgagaat gcaacatagg aatcactgag
     8461 cgtttccgat tcggactatg atgcaggttc cgcatgaaaa ccctatccaa gcagggggct
     8521 attccttatt gtctcccggg aaattgcccg gta
//
LOCUS       DASTJP010000870         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1245813, whole genome shotgun sequence.
ACCESSION   DASTJP010000870 DASTJP010000000
VERSION     DASTJP010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245813"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFQ23_25190"
     CDS             complement(<1..1062)
                     /locus_tag="VFQ23_25190"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909968.1"
                     /translation="MGLKITVIGAGSSYTPELFADLAVETQPLDVEQVVLMDMNPERA
                     AFVARVSEKLIKDSGQNIRVTGTADLEEAVRGADFIMLQIRVGGLAARVRDEKIPMEL
                     GMVGNETTGAGGFACALRTITATLDIARTIESLAPDAWIFNLSNPAGIVTETLLKRTS
                     LKVMGFCNIPINTTYALADVLGVSPAKVQLDSFGLNHLSWVRRVLVDGEERLQPLIAE
                     TCDADSILYRRGLVDDFMEPEFFRTLAMIPSWYVRYFYFPEQTLEEDRESGHSKGEED
                     MQREARLHEIYSMTGYDEEARRILSSKGGAQYYLPVLQAMQAIVNDSGEVVVADVRNG
                     TALPDLPTNVCVEVPTRVYR"
     gene            complement(1088..1939)
                     /locus_tag="VFQ23_25195"
     CDS             complement(1088..1939)
                     /locus_tag="VFQ23_25195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9909969.1"
                     /translation="MKRRRKILGLGIGGFLLLFAFLYFFPYIWMTAMSFKADGELFSG
                     KVFPEQPTLEQYQRLFYGYQYKDVTLTIPYWTYYKNTIIVTAISMVLVLFFDAMAAYG
                     FAKFNFRGKTLLFWLMIGTMMLPIYATLIPSFWLFSKLNWINTYHALILPGVVDAYGI
                     FLLKQYMETIPNELLDAAKVDGAGAFRRFWQIVLPLSRPALGTLAVFRFLFVWNDYLW
                     PLVIIRDDTMYTLTLGIANMQQRQGMVVWGQQMAAAVLATLPVLILVLVMQKQFLRGL
                     TAGAFKA"
     gene            complement(1970..>2367)
                     /locus_tag="VFQ23_25200"
     CDS             complement(1970..>2367)
                     /locus_tag="VFQ23_25200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9909970.1"
                     /translation="VMLIWAGLGWDSLIITAGLRSIPDEVYDAGKVDGTNGWTQFWYI
                     TLPLLQPTLLFVLVTGVIFLWGLFAQPSILTGGGPLRQTQTIAMYLYDVGFRYHKFGY
                     ASAIAMILSLIMFLSSYLNFRFVRAEAEY"
BASE COUNT          631 a          627 c          576 g          533 t
ORIGIN      
        1 gcggtagacc cgggtgggga cttccacaca gacgtttgtt ggtaaatcgg gtagtgcggt
       61 cccgttacgc acgtccgcaa cgacgacctc tccactatca ttaacaatgg cctgcattgc
      121 ctgcaggacg ggaaggtaat actgtgcacc gcctttcgac gacaggatac ggcgagcttc
      181 ttcatcgtaa ccggtcatgg aatagatctc gtggagccga gcctcacgct gcatatcttc
      241 ttcacccttg ctgtgaccag attctcggtc ttcctctaga gtctgctcgg ggaaataaaa
      301 gtagcggacg taccagctgg gaatcatcgc cagagtgcga aaaaattctg gttccatgaa
      361 atcgtctaca agaccgcgcc gatagaggat ggagtcagcg tcgcaggtct cagcgatcag
      421 gggctgcaat cgttcctcac catcgactaa aacgcgccgg acccaactca aatgattcaa
      481 cccaaaggag tccaattgaa ctttggcagg agaaacaccc agaacatcag ccagtgcata
      541 ggttgtattg atgggaatat tgcagaaccc catcaccttc aggctcgttc tcttgagcaa
      601 agtttccgtg acaattccag cgggattgct gagattgaag atccaggcat ccggcgccag
      661 gctttcgatc gtgcgtgcaa tatccaatgt cgccgtaata gttcgcagcg cacatgcgaa
      721 gcccccagca ccggtcgttt cgtttccaac catgcctaat tccattggaa ttttttcgtc
      781 acgcacacgg gcagcgagcc caccgacgcg gatttgcaac atgataaagt ctgccccccg
      841 gacggcttct tcaaggtccg ctgtccctgt gacccggata ttctgaccgg agtcctttat
      901 cagtttttca ctgacgcggg caacgaatgc tgctctctcc ggattcatat ccatcaggac
      961 gacctgctcg acgtctagcg gttgcgtttc taccgccaga tccgcaaaca gctcaggtgt
     1021 ataactgctg cccgccccaa tgacagtgat tttaagaccc atattttctc tccaaaattg
     1081 ttgagtgtta tgctttgaac gcgcccgcgg tcaggccgcg caggaattgt ttttgcatga
     1141 ccaacactaa gatcaacact ggcagcgtcg ccaggacagc agcggccatc tgttgtcccc
     1201 acaccaccat gccctggcgt tgctgcatgt tggcgatacc gagggtcagc gtatacatgg
     1261 tgtcatcccg gataattaca agcggccaca ggtaatcatt ccagacaaat aaaaatcgga
     1321 aaactgccag ggtcccaagt gccggacgac tcaacggtaa gacaatttgc cagaaacggc
     1381 ggaacgctcc agctccatcc actttggctg catccaacaa ttcgttcggg atcgtttcca
     1441 tatattgttt gagcaagaag atgccgtacg catccaccac ccccggcaga atgagcgcgt
     1501 gataggtgtt aatccaattc agctttgaaa acaaccaaaa ggaagggatc agggtggcgt
     1561 aaataggaag catcattgtc ccgatcatca gccaaaaaag cagcgtcttg ccgcggaaat
     1621 taaacttggc aaaaccgtag gcagccatcg catcgaaaaa gagaaccagt accatgctaa
     1681 tcgcggttac gatgattgta ttcttgtagt atgtccaata cgggattgta agtgtcacat
     1741 ccttatattg ataaccatag aaaagacgtt ggtattgttc caacgttggc tgttcaggga
     1801 aaacctttcc tgagaaaagc tctccatccg ccttgaatga catcgccgtc atccagatat
     1861 atgggaaaaa gtagagaaaa gcaaagagaa ggaggaagcc tccgatacct aatccaagta
     1921 ttttgcgcct acgtttcatg tcaaatcctc atttctagtg gcgtacgggc tagtactccg
     1981 cctccgctcg aacgaaccgg aagttcaggt aacttgatag gaacatgatc aaggatagaa
     2041 tcatcgcaat ggccgaggca tagccgaatt tatggtatcg gaagccaaca tcgtatagat
     2101 acatagcaat ggtctgggtc tgtcgtagag gaccgccgcc cgtcagtatc gagggctgag
     2161 caaataatcc ccacaggaag atcacgccag ttactaggac aaagagcagt gtgggttgaa
     2221 gaaggggaag ggtaatatac caaaactgtg tccagccatt cgttccatcc acctttccag
     2281 catcatagac ctcatctgga atggaacgta gaccagcagt gatgatcaac gagtcccagc
     2341 ctagcccagc ccagatcagc atgacca
//
LOCUS       DASTJP010000871         5012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1035487, whole genome shotgun sequence.
ACCESSION   DASTJP010000871 DASTJP010000000
VERSION     DASTJP010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035487"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="VFQ23_25205"
     CDS             <1..1589
                     /locus_tag="VFQ23_25205"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9909971.1"
                     /translation="IAHYVAKLEWAIAILFGALMTVTGPTVVQPLLKRIHISKRIKTI
                     LEGEAILIDPIGAILAVAVLDVVLQLSISRQVSVVGALWGYFGRLAIGIIVGGASALL
                     LARLMKVPRLIPPEISNLVALGMVWASFGIAESIQGEAGIMSSVAMGLVLQREAVPGE
                     RQLRHFKESLTTLSISTLFVLLAANLSLLTVLSEGYRGILTVFLIMFVARPISVCVAT
                     LGTALNWKEKLFISWIGPRGIVAASVASLFAFTLVGAGVEEGQRLLALTFLAIMMTVT
                     IQGLTASSVSRLLGVHTMEGRKAIIVGSGPLARAIAKAIKEHGRPIALVDTNQIMVEL
                     AQQDGLEAFHGNALEDELLEQTDIDEAETLLAVTPNSEVNVLVAQMAYDNFGIRRAYP
                     SIKNPERGANLRLLDRTGGKLAFGRPLDILAWEYVTSHIDRIDMEVHSNWNGKMVLAL
                     KFPEQVIPILRIRGTSTEIVHANQVWQVGDRVIFLTNVSPEKVMSTLKLECISKMVDF
                     GPNFCWERTDGSQRRAMGSG"
     gene            1555..2327
                     /locus_tag="VFQ23_25210"
     CDS             join(1555..1885,1885..2327)
                     /locus_tag="VFQ23_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611655.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET9909972.1"
                     /translation="MDLKDEQWAVVESLLPKVERRADGKGRPRVEDRAILNGILWILR
                     TGAPWHDMPNRYPSYQTCHRRFQEWVEKGVFEEVLRALVKDVKERGGLDLTECFIDGS
                     FVIAKKGVPGVGKTKRGKGTKLMAVADGTGFPVAISVGSASPHEVKLVEETLDKRFTE
                     EKPERLIGDRAYDSDPLDASLHKQGIELIAPHKSNRKKPATQDGRVLRRYKRRWKVER
                     LFAWLQNFRRIQVRHDRSLNNYLAFVLLGCIIIFLRNHF"
     gene            complement(2461..2616)
                     /locus_tag="VFQ23_25215"
     CDS             complement(2461..2616)
                     /locus_tag="VFQ23_25215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909973.1"
                     /translation="MSAIILATKLHIPPGRTSVIPRHRLIERLNDDLHRKLILVSALH
                     NLGKLLW"
     gene            complement(2782..3006)
                     /locus_tag="VFQ23_25220"
     CDS             complement(2782..3006)
                     /locus_tag="VFQ23_25220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909974.1"
                     /translation="MAPMVHGLEAKYFGKIIFSYLDADDPNTFDFQRTLGFYYQPEIY
                     LLDANGNLLQKWVGYTSEQEFETVFAQYIQ"
     gene            complement(3022..3327)
                     /locus_tag="VFQ23_25225"
     CDS             complement(3022..3327)
                     /locus_tag="VFQ23_25225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909975.1"
                     /translation="MKLRFLLSLSLFAVLLLSSCAPASTESVVLTTPAEANTPPVDAP
                     SPTLEAVLQPTLELTPTQAVDVLPVATSRGPELHATDPTTVSLATGQLHFVEFFRFT"
     gene            3570..>5012
                     /locus_tag="VFQ23_25230"
     CDS             3570..>5012
                     /locus_tag="VFQ23_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9909976.1"
                     /translation="MAGFKLTYGNMFNPPEELHIGFDKAVAKLKSNLGKEYGMIIDGK
                     DVFADEKFEDYSPVNTEWLLAVLQKGNASHANSAIAAARKAFPGWSHTPWKERVRLVR
                     KACALIEQRIFELGASMALEVGKNRMESLGDVQETADLMYYSAQMMEENDGFIKAMGK
                     DPLTGYDSTNTSILRPYGVWLIVSPFNFPFALTGGPTGAALVAGNTVVIKPASDTAWI
                     VRLYAECLRDAGIPDGVFNFVTGPGSTLGQALVDNRDVNGATFTGSFDVGMKLYRDFA
                     QRNYIRPIILELGGKNPAIVSRNANLEDAATGIYRSAFGLQGQKCSAASRVIVEEPVY
                     DELVSRLKAITDKLVIGDPTERSTYLGPVINQSSYNDFKNYTEEINQAGGQFLTGGHV
                     KTGGMYDKGYFVEPTFVTDLPFEHRLWQQEMFLPISTIGKVQNLDEAMRIANNVNYGL
                     TAGFYGSPAETDWFFDNIQAGVTYANRPQGA"
BASE COUNT         1302 a         1162 c         1320 g         1228 t
ORIGIN      
        1 tcattgcaca ttacgttgcg aaactggaat gggcaattgc gatcctcttc ggtgctctta
       61 tgacggtgac ggggcccacc gtagtccaac cgctcctaaa aaggatacat atttccaaaa
      121 ggattaagac catcttagaa ggggaagcca ttctgatcga tccgattggt gctattctgg
      181 ccgtagccgt tttggacgtt gtcttacagc tttcgatcag tcgtcaggtc agtgtggtcg
      241 gcgccctttg gggctacttt ggtcgactcg caattggcat aatcgtcggc ggagccagcg
      301 cactgcttct tgcgcgcctt atgaaagttc cgcgcttgat tccgcccgag attagtaatc
      361 tcgtcgcgct ggggatggtc tgggcatcat ttggaattgc cgaatcgatc cagggtgagg
      421 cgggaattat gtcttcggtc gcaatggggc tcgtccttca gcgcgaggcc gttccaggag
      481 aaagacagct acgtcacttc aaggaatcac tgacgaccct cagtattagc acgctgtttg
      541 ttctgcttgc agcaaatctg agcttgttga ccgttctttc agagggctac cggggaatct
      601 taacggtctt tctgatcatg ttcgttgccc gtccaatttc ggtttgcgtt gcaactttgg
      661 gcacggcatt gaactggaag gaaaaactct tcatatcatg gatcggacca cgcggaattg
      721 ttgccgcttc ggttgcttcc ttattcgcct ttaccctggt gggcgccggt gttgaagaag
      781 ggcaacggct gctggcatta acattccttg ctattatgat gactgtcact atacaagggc
      841 tcacagcatc gagtgtctca cggcttcttg gagtccatac catggaggga cgcaaagcga
      901 tcatcgtggg ttcgggcccc ctggcgcggg ccattgcgaa agccatcaag gaacatggtc
      961 gtccgattgc cttagtggac acgaatcaaa taatggtcga gttagcccag caggatggac
     1021 tggaagcatt ccacggtaat gcgctggaag atgaattgct ggaacaaacc gatattgacg
     1081 aagcagaaac tctgctggct gtcacgccta attctgaagt taatgtatta gtggcacaga
     1141 tggcttatga caattttggt atacgacggg cgtatcccag catcaagaat ccagaaagag
     1201 gggcaaatct aagattacta gaccgaacag gaggtaagct cgcctttggc aggccccttg
     1261 atatccttgc ctgggaatat gttacgagcc atatagacag aatagatatg gaagttcact
     1321 caaattggaa tggaaaaatg gtcttagcac tcaagtttcc agagcaagta attcccatcc
     1381 ttcgcattcg aggcacaagc acagaaattg tccatgctaa tcaagtctgg caagttgggg
     1441 acagagttat cttccttacc aatgtatcac cagaaaaagt aatgtcaacg ttgaaactag
     1501 aatgtatctc aaaaatggta gacttcggtc caaacttttg ttgggagcgg accgatggat
     1561 ctcaaagacg agcaatgggc agtggttgaa tcattacttc caaaagtgga acgtcgagca
     1621 gatggcaagg gacgtccgag agtagaggat cgagcgatcc tgaatgggat cttgtggatc
     1681 ttgcgcacgg gagcaccctg gcatgacatg ccgaatcgat atccatcgta ccagacctgc
     1741 caccgtcggt tccaggaatg ggtcgagaaa ggcgtgtttg aagaagtctt gcgagccctg
     1801 gtgaaagatg tgaaggagcg aggtggtttg gatctgacgg agtgtttcat cgatggcagc
     1861 tttgtcattg ccaaaaaagg ggtgctgggg tgggaaaaac caagcggggc aaaggtacaa
     1921 agctcatggc agtggcagat ggcactggtt ttcctgtcgc catatccgtt ggcagtgctt
     1981 cgccgcatga agtgaaattg gtggaagaga ccctggataa gagatttacg gaagaaaaac
     2041 ccgaacgatt gattggtgac cgagcgtatg atagcgaccc actcgatgcg agtttgcaca
     2101 aacaaggaat tgagttgatc gctccgcaca aatccaaccg caagaagccg gctacccaag
     2161 atggtcgtgt cctgcgaaga tacaaacgac gttggaaagt cgaacgcttg tttgcctggt
     2221 tgcaaaactt ccgaaggata caggttcgtc atgatcgtag tctcaacaac tatctcgcct
     2281 ttgtcttgct cggttgcatc attatcttcc tccgtaacca tttttgagat gacttctagt
     2341 gactgaagca ggccagtaat tcattttcaa aaaagttgaa gtgatctttc gacaaggtga
     2401 ttcggccgag aaactccact acctggtcag ctcgccgcct ggttgtagcg atccattcgc
     2461 ttaccagagt agttttccca aattatgtag ggcagaaacg aggatcaatt ttcgatgtag
     2521 atcgtcgttg agccgctcga tcagtcgatg acgaggaatg acactagtac ggcccggggg
     2581 aatgtgaagc ttcgtggcta aaataatggc cgacatcacg cgattatgaa cacatttatg
     2641 tcaggtcttg aagaagggtg ctggcaaaat cctttgcatg cagcagagaa aacgaatttt
     2701 tacaatggcc ttacaaaaga ctttgtgatt tagcccctgt ttaaaataga actccgcatt
     2761 ctattgaatg cggagttctg tctattgtat atattgcgca aatactgttt cgaactcttg
     2821 ctctgaggtg taccccaccc acttttggag taaattgcca ttggcatcca gaaggtaaat
     2881 ctcgggctga tagtaaaacc ctagcgtgcg ttgaaaatca aatgtgtttg ggtcatcagc
     2941 atccagataa ctaaatatga ttttgccaaa atatttcgct tcgagcccat gaaccatggg
     3001 cgccatagaa cggcaggtac ttcaggtaaa gcggaaaaat tcaacaaaat gcagctgccc
     3061 agtggctaga ctaaccgtcg tcgggtcagt ggcatgcagc tcgggaccac gcgacgtggc
     3121 aacagggagc acatccacag cctgagtagg agtcaactcc agagtgggct gcaaaacggc
     3181 ctccagcgta ggggagggcg catctaccgg tggtgtgttt gcctcagcag gggtggtcag
     3241 caccaccgac tctgtcgaag cgggagcgca ggaagagagc aataaaactg cgaagagact
     3301 aagagatagt aagaagcgaa gtttcattag aatttccttg aaaatttata acataaaatc
     3361 cttacgaaag gattaagaaa ctcatatttt tgaccatggt acgcaaatta atcctttctt
     3421 tgacggctgc gaggacctac gctatactag gttcgtcact tgtattctag tcagatgttt
     3481 ccatcatcaa agagccaatt gaatcgaaaa ttaccgaacc agagaactac ccgaccttca
     3541 aattacttaa atttttttca ggaggaagaa tggcaggttt caaactcacg tacggcaaca
     3601 tgttcaaccc acccgaggaa cttcatatcg gatttgataa agcggtcgcc aagctcaaat
     3661 caaacctcgg caaagaatac gggatgatca ttgatggcaa ggatgtattt gccgacgaga
     3721 aattcgaaga ctactcaccc gtcaatacag aatggctatt agctgtgctg cagaaaggca
     3781 atgcatccca cgccaattcc gccattgctg ccgcccgaaa ggcattccct ggctggagtc
     3841 acaccccctg gaaggagcgc gtccggctcg tgcgcaaggc ctgtgccctg atcgaacagc
     3901 gcattttcga gcttggtgcg tccatggcgc tggaggttgg caaaaaccgc atggaatcac
     3961 tcggtgacgt ccaggagacg gctgacctga tgtattactc cgcgcagatg atggaagaaa
     4021 atgatggctt catcaaagcg atgggcaagg atcctctcac aggatacgac tcgacaaaca
     4081 cttccattct gcgaccttat ggcgtctggc tgatcgtcag cccattcaat tttccgtttg
     4141 ctctgaccgg tggaccaacg ggtgcggccc tggtcgcagg aaacactgtc gtgatcaaac
     4201 ccgcttcgga tactgcctgg atcgtgcgac tctatgccga gtgtttgcga gatgcgggca
     4261 tcccggatgg tgtcttcaat tttgtgacgg gtccgggctc caccttgggg caggcgctgg
     4321 ttgacaatcg cgatgtaaat ggcgcgactt tcacgggttc atttgatgta gggatgaagc
     4381 tgtatcgaga ttttgcgcag cgcaattaca tccgcccgat tattctcgag ctgggtggca
     4441 agaacccggc cattgtttca cgcaatgcga atctcgaaga tgctgcgact ggcatttacc
     4501 gatctgcctt tggtttgcag ggacaaaaat gttccgcggc ctcacgcgtg atcgttgaag
     4561 aaccagtcta tgatgaattg gtatcgcggc ttaaagccat cacagataaa ctcgttatcg
     4621 gtgacccgac tgaacgtagc acctatttag ggccggtgat caatcagagc tcttacaatg
     4681 atttcaaaaa ctataccgag gagatcaatc aggcgggcgg acagttcctg accggtggtc
     4741 atgtaaaaac cggcggtatg tatgacaaag gttacttcgt cgaaccgacc ttcgtcacgg
     4801 acctgccgtt tgagcatcgg ctgtggcagc aggagatgtt cctgccgatc agcacgatcg
     4861 gtaaggttca aaatctcgat gaagcaatga ggatcgccaa taacgtaaat tatggtttga
     4921 ccgctggttt ctatggatcg cccgccgaga ccgattggtt ctttgataat atccaagcag
     4981 gcgttacata tgcaaatcgc ccacagggtg cg
//
LOCUS       DASTJP010000872        16337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1142219, whole genome shotgun sequence.
ACCESSION   DASTJP010000872 DASTJP010000000
VERSION     DASTJP010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16337
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142219"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="VFQ23_25235"
     CDS             complement(<1..1186)
                     /locus_tag="VFQ23_25235"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9909977.1"
                     /translation="MPPRRRLNLFAFLPETDALFGMLIMASIWLALFLGPAFFLVFGF
                     ESSLANTSFTTGGLEATRGYLPTICFSSIAISGVLVVAAIFYFRHPYEIRQRRKIESI
                     SEKDQEIQAQVTHLAIQSGIEPPKIEMPASGLRGTDAQAFGVGKMKRIALDGGLRILR
                     KTKPDVFQAIIQHELAHFANQDIGKSYFSDALWKSIRLVIMLPFVIGLLGYILLAVYL
                     GISNDNLLEQLTGPIQSFAGLIIQFGFVLVMAGSIWLRLLRTREFYADWRAVLWGAKN
                     GLSKIFQEEVEKEKSNARFQLYRFHPEANERLAAIENIEILFRFSPMMIFLSGLLLSM
                     LFVGLYFSLAAVLAVAGVLQAIRDTSTGLVYWLARGTLWFGIISTFLVTLGLSGWLIN
                     GVL"
     gene            complement(1193..1660)
                     /locus_tag="VFQ23_25240"
     CDS             complement(1193..1660)
                     /locus_tag="VFQ23_25240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909978.1"
                     /translation="MWNDTPESKQLVTEVSKEIVAEIAPEELEFIDELMEDYYASPAV
                     PQNRGNPLEFGGDLLAAATPVVAMALQTLFNFILNEVWVSAQKEGAVVIAQKMKTLLN
                     PVKEKTEPALGLTQEQLEKAKKLIRKEAIRGGMKPKKAEDLALKIVGRIALTA"
     gene            1790..2293
                     /locus_tag="VFQ23_25245"
     CDS             1790..2293
                     /locus_tag="VFQ23_25245"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET9909979.1"
                     /translation="MDIHFFIVMGVSGSGKTTVGKALASELGWEFYDADDFHPPENIS
                     KMESGIPLTDADRAPWLTALRNLIESCLKQNRPGVLACSALKERYRQKLMNDNDDIQL
                     VYLKGSYELIWSRMSARTDHFMKPEMLKSQFESLEEPVNALTVDISQSAEEILQDILR
                     RISNEQL"
     gene            2290..3444
                     /locus_tag="VFQ23_25250"
     CDS             2290..3444
                     /locus_tag="VFQ23_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9909980.1"
                     /translation="MNSLKNQFLLDPTVVFLNHGSFGATPKPVFREYQRWQRDLEKQP
                     VEFLSSTRRFASLMANARAVLGTYLGTNAENLVYTQNVTISLNIVARSLDLGSEDEVL
                     STDHEYGAIDRIWRFLSKERGFRYINQRISTPIESKESFVEEFWQGVSPRTRVIAISH
                     ITSSTAIIFPIHEIIRRAREAGIITVIDGAHVPGQLPLHLDSLGADFYGGNLHKWLCA
                     PKGAGFLYARPNVQHLLKPLVVSWGYESEAPSGSTFIDEQEWWGTRDVAAFLAVPAAI
                     EFHEKHNWETVRHQCHELARDAQNRIGQLTGLPPLHPPADDWFGQMFTAPLPPETDIG
                     TLKARLYDEFRVEVPLVEWNGNKLIRVSVQGYNTRRDIDQLCRALTSLLN"
     gene            3456..4070
                     /locus_tag="VFQ23_25255"
     CDS             3456..4070
                     /locus_tag="VFQ23_25255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909981.1"
                     /translation="MKMYALRINMVVVLFLVAACTPGAAPTQEAPTVESQPTAGEKFH
                     SLDTRTGIEAIDFVLTAVESGDPQSLRDLFSYTQTACMTVNALGGPPACRDGEAEGTS
                     VEVLPMLGPEGSYLYQDEIGAWPGLNAQGIYAVFQVSESAYSDEYYPKGDYGVILVGK
                     DENAVLQVNGSGIVRIDYILDLSPEALNAVLERSADELILAPIE"
     gene            4240..4440
                     /gene="rpmF"
                     /locus_tag="VFQ23_25260"
     CDS             4240..4440
                     /gene="rpmF"
                     /locus_tag="VFQ23_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET9909982.1"
                     /translation="MPPHPKRKHSKSRRNMRRAHDALQPRNLTACSNCGSMRLPHTVC
                     PNCGFYEGREVVEIKKEKKKES"
     gene            4630..5574
                     /gene="fabD"
                     /locus_tag="VFQ23_25265"
     CDS             4630..5574
                     /gene="fabD"
                     /locus_tag="VFQ23_25265"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET9909983.1"
                     /translation="MKLNPQTTAFVFPGQGSQAVGMGKDLAAQFPVSKEIFEEADAAL
                     GFSLSQLMWEGPDSELNDTINTQPALYVHSIAAYKTFSNLYPNFKPVAVAGHSLGELS
                     ALTVSGALSFSDGLRLVHTRGELMKRAGELNPGGMAAILGVDIPTLDKVCAEASAAEE
                     IVQVANDNCPGQVVISGHKPAVERAMAGAKSAGAKRALPLPISIAAHSPLMDSIQSEW
                     NAAVDACTMSDPGIPVISNVHAKAMTVADELRADIKAQMQSRVRWTESVQMMLGSSIQ
                     SYVEVGSGEVLLGLIKRIDASALRFALGNPADFAALNE"
     gene            5593..6027
                     /locus_tag="VFQ23_25270"
     CDS             5593..6027
                     /locus_tag="VFQ23_25270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909984.1"
                     /translation="MADVNARVQRVVEELTGNEALLEMLETEAATEMLNWGIDMAKSL
                     VKKTDDMDEPAANLALLPRLKEIRQSMRSIGNWAAGKYTDIASRAELRDKLLERFRVI
                     FGERMILPTTAEMDRLLNEADDENRNPYQLISQLRELLETKG"
     gene            6037..7227
                     /locus_tag="VFQ23_25275"
     CDS             6037..7227
                     /locus_tag="VFQ23_25275"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9909985.1"
                     /translation="MPKKRRKSKKGGAATLSGLIVLIIIGVYYFLTGTNTGGVFGSGT
                     ATPSPTQTPVASLPTAVSASGAWWEVYFTDPLNINNPDDWEGSIEGRLIEKINAAQTS
                     IHIVSFEFDLTRVAEALIAAEQRGVDVRWVTDDEHGIEADGEPGHGQFAMLDDAGIEV
                     RSDTRSALMHNKFWIFDGQIVWTGSTNITENGIFKQDNNTIVIQSAALATIYEREFQE
                     MWDGQFGPTSPSSLDEQITNVDGSQIVVVFTSEDPALEQAIIPLVKSAKQSVRFMAFS
                     FTDFPLAEALIERSRSGVDVSGVFERLGSDTEASELKTLICADVPARRDGNPSFLHHK
                     VIVIDERIVVTGSMNYSTNAEESNDENVLIIDNAEIARLYTQEFERVWSLGADPELDT
                     FACG"
     gene            7320..8045
                     /locus_tag="VFQ23_25280"
     CDS             7320..8045
                     /locus_tag="VFQ23_25280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9909986.1"
                     /translation="MNARPKSGRRGASRTPAPSQVPTMDLGSLFNVASQALTQNQSSL
                     NQADVENQNHGDNMVQVFNMISQVMANQQGAPPSTQLRRASQYLAKNGTSGSSQVYSQ
                     ALAQASKQFRGKSAVTPDNAMMLIQALLGSGQQSMPKEASAELLGALLGGGGQSSQGT
                     ELLETLLGGKGKQGGLDVATLLNAGMTFMNAKQQGQDNMQAALTALMSTGPMAQKPHR
                     QQSGQLVANALLQALASGMSNRR"
     gene            complement(8141..8617)
                     /locus_tag="VFQ23_25285"
     CDS             complement(8141..8617)
                     /locus_tag="VFQ23_25285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9909987.1"
                     /translation="MSDEKKSLFDKAIDALTDRDEKEAAEKARLEAAGAKEAAARKAM
                     ADKAAAEKAAEDKAAAEKVAAQKAATEKAADEREAAVKAAMNKAAAAPKKGIVTVRSL
                     RVRADHNTTSEVVAGLVDGNEVTILDTWTDGKDTWAKLENGWAAMVYDGDTYIKPA"
     gene            9117..10763
                     /gene="pckA"
                     /locus_tag="VFQ23_25290"
     CDS             9117..10763
                     /gene="pckA"
                     /locus_tag="VFQ23_25290"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET9909988.1"
                     /translation="MNNILEIKTPAETFAQARRADFDLSNQGIRDARLAYWNLSTEAL
                     FEEAVFRGEGYAARGGAFVAHTGRHTARSASDKFVVKHVDSENNIWWGTYNRPFDGGK
                     FDGLYDRMSEYLQGKDVFVQDVYAGADENYRLPVRFITEQAWHSMFLRNMFILPQTME
                     EYKDFVPEFTIVAAPGFQADPAIDGTRSDTFIILNFERRLAIIGNTAYAGETKKTVFT
                     LLNYLLPLQGVLSMHCSANVNPDDQHDVALFFGLSGTGKTTLSADPTRRLIGDDEHGW
                     SEDGVFNFEGGCYAKVIGLSESAEPQIYATTKMFGTILENVPFNPVTREIDLNDDSIT
                     ENTRASYPLDFIVNAVPEKKAGHPKNIILLTCDASGVMPPIARLTPNQALYQFISGYT
                     AKVGGTEVGLGKEPAITFSACFGGPFMVHHPSKYAELLKEKIERYGVNCWLVNTGWVG
                     GPFGVGKRISIKHTRALLNAALTGQLAEVEYDRDPVFGFEVPRTCPGVPDEVLKPWSS
                     WPNREEYDQKYKQLAQKFIENFAKFESETSQEVIDAGPKV"
     gene            complement(10902..12053)
                     /locus_tag="VFQ23_25295"
     CDS             complement(10902..12053)
                     /locus_tag="VFQ23_25295"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9909989.1"
                     /translation="MNFDLTDEQKLWQKTIHEFVAKEVQPQAHEVDITSEFNWTATRK
                     MGPIGLLGLSIPEEYGGSGVDAISAAIAIEELGWGCGSTALAIAAHNGLGTTPVVLYG
                     SERLKEEWLPQVSNGKNKLAALALTEPGAGSDLQGGVVTKAKKDGNDWVISGAKMWCT
                     NASIAEYIITLVRTDTAGGSRSLSQILVPTNTTGLKIGPAEKKMGLHGSPTHAVTYED
                     VRVPLGNLIGEEGKGLHQTLSTLDGGRIGIGAISVGLAQAAFEHAVNYARERRAFGKP
                     IAEQQAIQWMLADAATEIEIARTMLYKAAWLKEQKRPYNKEAAMAKMFATEMAERVCR
                     NAIQIHGGYGYSSEYPVERIYRDARLMTIGEGTSEIQRMVIARHVLASE"
     gene            12249..12992
                     /locus_tag="VFQ23_25300"
     CDS             12249..12992
                     /locus_tag="VFQ23_25300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9909990.1"
                     /translation="MANKKKIEEREAELRAQQTERTIRIVGIGVVALIVLTLIFMYFS
                     NRTDAVPTEVPLTEVPQTELPLTEPPQAETPVENNFSYDAYPPMTIDVSKEYFADVKM
                     AEGGEFTIQLFADKAPKTVNSFVFLARAGYFDGLTFHRVLQDFMAQGGDPTGTGMGGP
                     GYEFENEESDLTFDKEGVVAMANAGPDTNGSQFFITFGPTPHLNGGYTIFGQVIEGMD
                     VVKAITLRDPDANPTFTGDVMESVTITEQ"
     gene            complement(13064..13411)
                     /locus_tag="VFQ23_25305"
     CDS             complement(13064..13411)
                     /locus_tag="VFQ23_25305"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HET9909991.1"
                     /translation="MTEETVNPIKWTIHDTHPDRVQVSRDRLSEVVDPEIGMNIIQLG
                     LVRDIVIENDIARLKMILTTPFCPYGPAMIEMTKAKAVEALDMPVTIEMGMDMWDFSM
                     MEDPSALDWGMYA"
     gene            complement(13450..14070)
                     /locus_tag="VFQ23_25310"
     CDS             complement(13450..14070)
                     /locus_tag="VFQ23_25310"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET9909992.1"
                     /translation="MKSTKDKILQTLLRRPKITINDLAEAVGINPISVRHHLTNLQME
                     GLVGADEERHGVGRPRLVYSLTEDGMEKFPTKYLRLTTRLLTRMKETMPAPVVSQLFG
                     QIAEDLANEYSSQIQGLSMEERLEFVKELLAQEGFTVEWEKKGGEYQIHEISCPYYQI
                     GIAHPEVCTVDQTLISKMLALPVNKIQCILDGSAHCTYVVNAGSKN"
     gene            complement(14240..>16337)
                     /locus_tag="VFQ23_25315"
     CDS             complement(14240..>16337)
                     /locus_tag="VFQ23_25315"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9909993.1"
                     /translation="IIVNTSSSTVGAQAAWTPTPVRDPNDPEIVGGAASDPGEWPWQV
                     ALIGGSDPDLLGQFCGGSLITSQWVVTAAHCVYDFSTNPPTPINSADIDVVAGVYNVV
                     TPVAGFQRRNVSQIIRHASFNPTSPNLEFDIALLQLGSPITIGYTGQIKTSLVPLVSS
                     SVGTLAGTNAWVTGWGAIASGGPLADRILEVQIPVITNTNCNNAYNPFGFTIASSMLC
                     AGSAGKDACQGDSGGPLVVPSGNKWTLAGIVSWGIDCGVNPGVYTRVSDFVSWINTSV
                     PDRQNVFIGDQLKGKYGLIQNQSIRDGYVVDDGPAQVVSTNGASVITALRVIWKEPGP
                     RYSYSEMMGLPVEQLSNEYWFPWYNFAAPNSMDQGFRIANVDSGANTVEVRVGTTLLD
                     TIALGGGDSVRVGYPAVDNGPIKILCTTCSNTGTDKIIAALRVIWKEPGFRASYSEMM
                     GLPREQLSSEYWFPWYNNAAVNSMDQGFRIANVDAGPNTVEVRVGTTLLDTIALGGGA
                     SVRVGYTIDNGPIQIRCTTCSTGTDKIIAALRVIWKEPGFRASYSEMMGLPAEALSTE
                     YWFPWYNNLDTASMDQGFRIANVDTVGHTVRVFVGAAQVGTDLSLAAGGSVRVGFSVN
                     NGPIRIVCTTCNAGNANDRIIAALRVIWQEPGFRASYSEMMGLPAEQLSTEYWFPWYN
                     NASAASMDQGFRIGVP"
BASE COUNT         4070 a         4072 c         4045 g         4150 t
ORIGIN      
        1 gtaagacacc gttaattagc catcctgaca ggcccaatgt cacaaggaag gtacttatta
       61 tgccgaacca caatgtccct ctcgcaagcc aatataccag gcctgttgat gtatcacgaa
      121 tcgcctgtag cactccagca actgctaaaa cggcggcgag cgagaaatac aatccgacga
      181 atagcataga taacagcagg ccggatagaa aaatcatcat cggcgaaaat cgaaaaagaa
      241 tttcgatgtt ttctatggca gccagtcgtt cgttagcctc cggatgaaac ctgtataact
      301 gaaatcgtgc atttgatttt tccttctcaa cctcctcctg gaatatttta ctcaatccat
      361 ttttggctcc ccataatact gctcgccaat ctgcataaaa ctcccttgtt cttaagagcc
      421 gcaaccaaat tgatcccgcc atcacaagca caaacccgaa ttgaataatc aagccagcaa
      481 aggattggat tggtccggtt agttgttcca gtaagttatc gtttgagata ccaagataaa
      541 cggccaggag gatatatcca agcagcccga ttacaaaggg tagcataatc accaggcgga
      601 ttgatttcca gagtgcatct gaaaaatagg actttcctat atcctggttg gcaaaatgag
      661 ccaattcatg ctggatgatc gcctggaaga catccggctt tgtcttccta aggatgcgca
      721 gacctccgtc cagggcaatc cttttcatct tgccaacgcc gaatgcttgc gcgtcggtcc
      781 ctctcaaacc cgatgcaggc atctcaatct tcggaggttc aattcccgac tgaatcgcca
      841 aatgagttac ctgggcttga atttcttgat ctttctctga aatggattct atcttccgtc
      901 tttgtcgaat ctcgtatggg tgacgaaagt agaaaatggc tgcaacaacg agaacgcctg
      961 agatggcaat gcttgaaaag caaatcgtag gcaggtaacc acgcgtcgct tccaatccgc
     1021 ccgtcgtaaa cgaagtgttt gctaaactac tttcgaaacc aaataccagg aaaaatgctg
     1081 gtcccagaaa taaggccagc caaatggatg ccatgatcag catgccgaaa agcgcatccg
     1141 tttccggcag aaatgcaaac agatttaacc gtctgcgtgg aggcatggtt gctcaggctg
     1201 ttaatgcaat acgacccacg attttcaacg ccagatcttc ggctttcttc ggtttcatcc
     1261 ctccgcgaat ggcttctttt cgaatcagct ttttggcttt ttccaattgt tcctgtgtta
     1321 atccaagcgc tggctccgtt ttttctttta cagggttcaa cagtgttttc atcttttgag
     1381 cgatcacaac cgccccttcc ttttgcgcag atacccagac ttcattcaaa atgaagttaa
     1441 acagagtctg taaagccatc gcaacgacag gggtggcagc agccaaaagg tcgccgccaa
     1501 attcaagagg gttgccccta ttttgtggaa cagccgggct ggcgtaataa tcctccatta
     1561 actcgtctat gaattccaat tcttcaggtg caatttccgc aacaatttct tttgagactt
     1621 cggtgacaag ttgtttgctt tcaggcgtat cgttccacat attgactact cctacgtgct
     1681 tctaaataga ttttttgtgc agggtacagg tttgagaatt cgccacgcct tcactcggcg
     1741 aatgtatccg attttaccgg acttacgact acaatacatc atacttaaga tggacataca
     1801 cttttttatc gtgatgggcg tctctggcag cgggaaaacc acggttggta aggccctcgc
     1861 ctcagaactt ggctgggagt tttacgatgc ggatgatttc catcctcctg aaaatatctc
     1921 caaaatggag agcggaattc cgctcaccga tgcagaccgt gctccctggc ttaccgccct
     1981 ccgtaatcta atcgaatcct gcctgaaaca gaatcgacct ggtgtgttgg cctgctccgc
     2041 tctcaaggag cgttatcggc aaaagttaat gaatgacaat gatgatatcc agctcgtcta
     2101 cctaaagggc agttatgagt tgatctggtc gcgcatgtca gcgcgtaccg atcacttcat
     2161 gaaaccagag atgttgaagt cgcagtttga gtcgttagag gaaccggtga atgcgcttac
     2221 tgtcgacatc tcacaatcag cagaggagat tttgcaggac attttgagac gcatcagcaa
     2281 tgaacaacta tgaattctct taaaaatcaa tttctgctgg atccaactgt agtttttctc
     2341 aaccatggtt cattcggtgc cacgcctaaa cctgtcttcc gtgaatacca gcgttggcag
     2401 cgcgatttgg agaagcagcc tgttgaattt ctgagctcca ctcgccgttt tgccagctta
     2461 atggcgaatg cacgagcagt gctcggaact tatctgggta cgaacgcgga aaatcttgtt
     2521 tacacccaga acgtgacaat ttcactcaat attgtggcgc gttcactgga tttgggttct
     2581 gaggacgagg tgctttcaac agatcacgaa tatggagcga ttgaccgcat ctggcgcttt
     2641 ctctcaaaag aacggggctt tcgttatatc aatcagcgaa tatcaacacc gatagaatcg
     2701 aaggaaagct ttgttgagga attctggcag ggagtttcgc ccaggacgcg cgtgattgcg
     2761 attagtcaca ttacctcatc cactgccatc attttcccaa ttcatgagat cattcgccgc
     2821 gcgcgcgagg caggcataat cacagtgatt gatggcgcgc atgttccggg gcaattgcca
     2881 ttgcatctcg actcgcttgg cgcggacttc tacggcggta atcttcacaa gtggctgtgt
     2941 gctccaaaag gagcgggatt tctctacgcc cgtcctaatg tgcagcacct gttgaagcct
     3001 cttgttgttt catggggcta tgaatcagaa gctcccagcg gttccacttt cattgatgag
     3061 caggagtggt gggggacccg cgatgttgct gcatttctag cagtgcctgc ggcgatagag
     3121 ttccacgaaa aacataattg ggaaacagtg cgccaccagt gtcacgagct tgcacgagat
     3181 gcgcaaaaca ggattggcca attaaccgga ttgcctccgc ttcatcctcc cgctgacgac
     3241 tggttcggtc aaatgttcac cgcgcccctt ccaccggaaa cggacatcgg aactctcaag
     3301 gcaaggttgt acgacgaatt ccgcgtcgaa gtgccattgg tagagtggaa cgggaacaaa
     3361 ctcatacgtg tctccgttca aggatacaac acaagacgcg acatagatca gctctgtcgc
     3421 gccctgacat cgttgttgaa ttgaaaggat acaaaatgaa aatgtatgct ctaaggatca
     3481 atatggtggt ggtactgttc ctggtagcag cctgcactcc cggagcggct cccacgcagg
     3541 aagctcctac cgttgagtcc cagccaaccg ctggagagaa atttcattcg cttgatacgc
     3601 gaactggaat tgaagccatt gatttcgttc ttaccgctgt ggaaagtggt gatccccagt
     3661 cgttacgcga tctgttcagc tacactcaaa cagcatgtat gacagtcaac gccttgggcg
     3721 gaccgccggc ctgccgcgac ggtgaagcag aggggacctc ggtcgaagtc cttccgatgc
     3781 tcggaccaga aggcagctac ttgtatcagg atgaaatcgg tgcctggcct gggctgaatg
     3841 cgcaaggaat atacgctgta ttccaggttt ctgagagcgc ttattcagat gagtattatc
     3901 cgaaaggaga ttatggtgta attctggtcg gaaaggacga aaatgccgtc ttgcaggtca
     3961 acggtagcgg cattgtcaga attgattaca tcctcgatct ttctccggag gctctaaacg
     4021 cggtccttga acgcagcgca gacgaactga ttcttgctcc aattgaataa ttgaaatggc
     4081 cgctgtgggt cggctgcggg tacattttgc ccaccggaat ggcatctcat ccttgacgcc
     4141 tcttccgcct cccgctataa tctgcccgcc tcaatcgtaa attatgtagg ggcggacggc
     4201 tgtccgcccc tataacgtgt aaataaagga gaaagtttca tgcccccaca tccaaagcgc
     4261 aaacactcga agagccgccg caatatgcgc cgcgcacacg atgccctgca accacgcaat
     4321 ctgactgcct gctcgaactg tggatcaatg cgcctgccgc acacagtctg tccgaattgc
     4381 ggcttttatg aaggtcgcga agtggtcgaa ataaagaaag aaaagaaaaa ggaatcgtaa
     4441 cgacgagcgc tttgcgtctc gtcctggcgg gtcgtgacga acacgacccg tttgtgtttt
     4501 aattgatatt ctcgcgccga gcggagggac gcaatgaaat gaaggcccgc agacgaagcg
     4561 cgacttttca tgcaaaaaca ctcttcgtct gcacctctgg ctccgctcag cgcgaatact
     4621 aggagaccaa tgaaacttaa cccacaaact accgcatttg tatttcctgg gcaaggttcg
     4681 caagccgtgg gcatgggtaa agaccttgcc gcgcagtttc ctgtttcaaa agaaatattt
     4741 gaggaagcgg atgctgcact gggtttttcc ctctctcagt tgatgtggga aggacctgac
     4801 tctgaactta acgataccat caatacacaa ccggcattat atgttcattc tattgccgca
     4861 tataaaactt tttcgaatct ttacccgaac ttcaagccgg ttgccgttgc tggccattct
     4921 ctcggtgagc tttctgcgct gacagtatcg ggcgcattgt ccttttcgga cggtcttcgg
     4981 ctcgtgcata cgcgtggtga gttgatgaaa cgagccgggg aattgaaccc gggcggtatg
     5041 gcggctattc tcggtgtgga tattcccact ctggataaag tttgtgcgga ggcaagtgcg
     5101 gctgaagaga tcgttcaagt tgcgaacgat aactgccctg gtcaggtggt aatctcggga
     5161 cacaaaccag ctgtggagcg agcgatggcg ggggccaaat ctgcgggcgc gaaacgtgct
     5221 ttgccattgc cgatcagcat tgctgcacat tcccctctta tggattcaat acaaagcgaa
     5281 tggaacgcgg cggtagatgc ctgcacgatg agtgacccag gcatccccgt tatcagtaac
     5341 gtccatgcaa aggccatgac ggtggctgac gagttgcgcg ccgatatcaa ggcgcagatg
     5401 cagtcacgcg tccgctggac tgagtcggtg caaatgatgc tggggagcag tatccagtcg
     5461 tacgtcgagg tggggagcgg ggaagtatta ttgggtttga tcaaacgtat tgatgcatct
     5521 gcacttcgat ttgcattggg gaatccagca gactttgccg cgctgaatga ataatcggat
     5581 atacttttaa tcatggcaga cgtcaatgcg cgagttcaac gtgtggtcga agagcttacg
     5641 ggcaatgaag cactgttgga gatgctggag acagaagctg ctactgaaat gctcaattgg
     5701 ggaatcgata tggcaaagtc cttggtcaag aaaacagatg atatggatga acctgctgcc
     5761 aatctggctt tgttgccgcg cttgaaagag atacggcagt ccatgcgttc cattggcaat
     5821 tgggcagccg gtaaatacac cgacatagcc agtcgtgcgg agctgcgcga taaactgctt
     5881 gaacgatttc gagtgatctt tggcgaaaga atgattttgc caacaacggc ggaaatggat
     5941 cgactattaa atgaggcgga tgatgaaaat cgaaatccgt atcagttaat atcgcagtta
     6001 agagaattac tggaaacaaa aggttaagga gaaccgatgc cgaagaaacg acgcaaatca
     6061 aaaaagggag gcgctgcaac cttatccgga ttgattgtac ttatcatcat cggagtttat
     6121 tattttttga cgggtacgaa tacgggcggc gtatttggtt cgggcacagc aacgccgtca
     6181 ccaacacaaa ctcccgtggc gagccttcct acggcagtga gcgcaagcgg agcctggtgg
     6241 gaggtttact ttactgaccc gctcaatatc aacaacccag acgattggga aggctccatc
     6301 gaagggcgct tgatcgagaa gatcaatgcc gcgcagacca gtattcatat tgtatctttt
     6361 gagttcgatc taacgcgggt tgctgaagct ttgatcgcag cagagcagcg cggcgtggac
     6421 gtgcgctggg tgacagacga cgagcatgga atagaagccg atggggagcc agggcatggt
     6481 caatttgcca tgttggatga cgcagggata gaggttcgtt ctgatactcg ctctgcattg
     6541 atgcacaata agttctggat ctttgacggg cagatcgtat ggacgggttc gacaaatatc
     6601 acggagaatg ggattttcaa gcaggacaac aataccatcg tgatccagtc ggcagcttta
     6661 gctaccatct atgaacgcga gttccaggaa atgtgggacg gtcaattcgg accgacctct
     6721 ccatcctcgt tggacgaaca gataacgaat gttgatgggt ctcagattgt cgtcgtattt
     6781 acctctgaag accccgcgct ggaacaggcc atcatccctt tagttaagtc tgccaaacaa
     6841 agcgttcggt ttatggcgtt tagtttcacc gatttccctc tggcagaggc cctgatcgag
     6901 cgttcaagaa gtggcgttga tgtatccgga gttttcgaaa ggctcggtag cgatacggaa
     6961 gcctctgaac tcaagacact gatttgtgcg gatgtcccag cacgccggga cgggaatcca
     7021 agttttttgc atcacaaggt cattgtgatc gatgagcgaa ttgtcgttac cggttccatg
     7081 aattattcga ccaatgccga ggaaagcaac gacgaaaatg tcctcatcat tgataatgct
     7141 gaaattgcca ggttatatac ccaggaattt gaacgagtct ggagtttggg cgccgacccg
     7201 gaactggaca ccttcgcctg cggttaacga tcccttatga cctaaactgg tagaatacgt
     7261 ataagcgttt cgtttgttgt acgctttcaa caccctatcg attggaaagg atttctctca
     7321 tgaatgctag accaaaatcc ggcagaagag gagcaagtcg tacgccagct ccgtcacaag
     7381 tccccaccat ggatttggga tcgttattca atgtagcctc tcaggcgctg acgcaaaatc
     7441 aatcttcact gaatcaagcg gatgtcgaga atcaaaatca tggggacaat atggtgcagg
     7501 tttttaacat gatctcgcag gtgatggcaa atcagcaggg tgcccctcct tcaacacaac
     7561 tccgtcgggc cagccaatac ctggcaaaga acggcaccag tggttcgtca caggtctatt
     7621 ctcaggcact ggcacaggcc tccaaacaat tccgcggcaa gtcggccgta acgccggata
     7681 atgccatgat gctgattcag gcactgctcg gcagcggaca acaatccatg ccaaaggagg
     7741 cgagcgctga acttttaggt gctctgcttg gtggcggagg acagtcttct cagggtaccg
     7801 aattgttgga gactcttttg ggtggcaagg gaaaacaagg tggtcttgat gttgccacac
     7861 tcctgaacgc tggcatgacg ttcatgaatg cgaaacaaca gggacaggat aatatgcagg
     7921 cggccctaac agcattgatg tccacaggac ccatggcgca aaaaccgcac cgtcagcaat
     7981 cgggtcaatt agtagccaat gccctacttc aagcgcttgc ttccggcatg tcaaatcgca
     8041 gataaagaat ccaaccctct cctggtaagt gaaataataa gacctccgat tttcggaggt
     8101 cttatcattg ttacatcaag aggattgttg gatgatgctc ttaagcaggc ttgatataag
     8161 tgtcgccatc ataaaccatg gcggcccagc cgttttccag ttttgcccag gtatcttttc
     8221 catctgtcca cgtgtcgagg attgtcactt cgttgccatc caccagaccg gcgacgacct
     8281 ctgacgttgt gttatggtcc gcgcgcacgc gtagactccg aacagtcaca attccctttt
     8341 tgggcgcggc agccgctttg ttcatggcag cttttactgc ggcttctctt tcatctgcag
     8401 ctttctcggt agcagccttc tgtgcagcaa ccttttcggc ggcagccttg tcttcggcgg
     8461 ctttctccgc ggctgcttta tctgccatgg ctttccttgc cgctgcctct ttagcgccag
     8521 cagcttctaa tcgggctttt tccgcggcct ctttttcatc gcggtcggtc aaagcatcga
     8581 ttgccttatc aaacaggctc ttcttttcgt cgctcataat atttctcctt tttgttttga
     8641 tttgtgcggc aattcagatt atatactcga ttagagatac gtcaacgtag caggaattgg
     8701 tcgtatcgta aagctaccat cagaacttcc tggacacagc atgagttaac tcaacccgtc
     8761 tcatttcaac tattgatcgt atcttgctct atggataaag ccattattcc tcctgcctga
     8821 attaatagtc tgttaagtca attatgggct gattttcgct gttttcgaga atcggctacc
     8881 gggtggcaaa tccaaagatg ccctactgtg ttaagacaaa tgtcaccttt ctatcggacg
     8941 cgacctcgtg ggctgacata aatgaaactt ttgtcttcta agaaattatt gagggattgg
     9001 ttataattaa gtggtacgtg cgatattcag gcgaatttcc cgatagggat gcctgatgcg
     9061 atcgaatacc aggcgtgtgc agggaagccc ctgctcttat ggagaatcaa ggaataatga
     9121 ataacatttt ggaaatcaaa accccagcag agacatttgc gcaggcgaga agagctgatt
     9181 tcgatctctc caatcaggga attcgagatg cgcggcttgc ctattggaat ctctccacgg
     9241 aagccttgtt tgaggaagcc gtctttcgag gggaagggta cgctgcgcgc ggaggagcgt
     9301 tcgttgcaca taccggccgg catacggctc gttcggccag tgataagttc gttgtaaagc
     9361 atgttgattc cgaaaataat atctggtggg gaacctacaa ccgccccttt gatggcggga
     9421 agttcgacgg gttatacgat cgcatgtcag agtatttgca gggcaaggat gttttcgtgc
     9481 aggatgttta tgcaggtgcc gatgaaaatt accgcttgcc ggtacgcttc ataacggaac
     9541 aggcctggca cagcatgttc ctgcgtaata tgtttatcct gccacaaaca atggaggagt
     9601 ataaggattt tgttcccgag ttcaccatcg tggccgcgcc gggtttccag gctgatcctg
     9661 caatcgacgg gacgcgcagt gatacattta ttattttgaa ttttgagaga aggcttgcca
     9721 ttatcggcaa cacggcctac gcgggtgaaa cgaaaaagac agtattcact ttattaaatt
     9781 atcttctccc gcttcaaggt gtgctctcga tgcactgttc agcgaatgtg aatccggatg
     9841 atcagcacga tgtagcttta ttctttggct tgagcgggac cggcaaaacg acgctttctg
     9901 ccgacccgac ccgccgcctg atcggggacg atgaacatgg ctggagtgag gatggtgtct
     9961 tcaattttga aggcggctgc tatgctaaag tcatcgggtt atctgaatct gcagagcctc
    10021 aaatttatgc gaccacaaaa atgtttggga caatccttga gaatgttcca tttaacccag
    10081 ttacccggga gattgatctg aacgatgact cgattacaga aaatacacgt gcatcttatc
    10141 ctctggactt tattgtcaat gcggtcccgg aaaagaaagc agggcaccca aagaacatca
    10201 tcctgttgac gtgcgatgcc agcggcgtta tgcccccgat cgcgcgcttg actccgaacc
    10261 aggctttgta tcaattcatt tcaggttata cagccaaggt gggcggcact gaagtcggtc
    10321 ttggtaaaga gccagcgatc accttctctg cctgctttgg tggtccattt atggtgcatc
    10381 acccctccaa atacgcagaa ttgctcaaag agaagatcga gcgctatgga gttaactgct
    10441 ggttggtgaa caccggctgg gtgggaggtc catttggcgt cggcaaacgc atttcgatca
    10501 agcacacgcg cgccttattg aatgctgccc tgacgggtca acttgccgaa gtcgaatacg
    10561 atagagaccc ggtatttgga ttcgaagtgc cgcgtacctg tcctggtgtt ccggacgagg
    10621 tgcttaagcc gtggtcttcc tggccgaatc gtgaagaata cgatcagaaa tacaagcagt
    10681 tggcacaaaa gtttatcgag aattttgcga aatttgaaag tgaaacttcg caggaagtaa
    10741 tcgatgcggg accaaaggtc tgaactgaga aatcctggtg aatcaaaaat gcgactccgg
    10801 cagagtcgca tttttatttt accgtttgaa ggttctcatt tattcaagcc gccatatgta
    10861 agctttcggt tattacactt taaacagcca caaatagtta cctactcgct tgccagtaca
    10921 tgcctcgcaa tgaccatccg ctgtatttcg ctggtacctt cgccaatcgt catcaagcgc
    10981 gcatcgcgat aaattcgttc gacgggatat tcactgctgt atccatatcc accatggatt
    11041 tgaatcgcat tgcgacaaac gcgctcggcc atttctgtgg caaacatctt tgccatggca
    11101 gcttctttgt tatagggtcg tttttgctct ttgagccacg cggccttata aagcattgta
    11161 cgcgcgattt cgatctcggt tgccgcatct gccagcatcc attggatggc ttgttgttca
    11221 gcaatcggct tcccgaaggc tctacgttcg cgtgcataat taacagcatg ttcgaatgca
    11281 gcctgcgcaa ggccaactga aattgcaccg attccgattc tccctccatc cagcgtggat
    11341 agggtttgat gtaatccttt gccctcttcg ccaatgaggt tgcccaacgg cacgcgcacg
    11401 tcttcgtacg tcactgcatg agtgggggag ccatgcaacc ccatcttttt ctctgctgga
    11461 ccgatcttga gtcccgttgt attagtgggg acaaggatct ggctcagtga acgggagcca
    11521 cccgcggtgt cggtacgaac gagggtgatg atatactccg cgatcgaggc gtttgtacac
    11581 cacatcttcg cgccgcttat cacccagtca tttccatcct tttttgcttt tgtcacgaca
    11641 ccaccctgta ggtcagaacc ggctcccggc tcggtcagcg cgagggcagc tagcttgttt
    11701 tttccatttg aaacctgggg aagccattcc tccttcagtc tctcacttcc atagagaacc
    11761 actggcgtgg ttcccagtcc attgtgagcc gcgatcgcaa gggcagttga gccgcagccc
    11821 cagccgagtt cttcaatcgc aatcgcggcg cttatggcat cgacacctga accaccatac
    11881 tcttctggaa tacttaaacc gagcaatccg atcggcccca tcttgcgagt tgcggtccaa
    11941 ttgaattcgc tggtgatatc tacctcatgc gcttgcggtt gaacttcttt cgccacaaat
    12001 tcgtggattg ttttttgcca gagtttttgt tcatctgtta agtcaaaatt cattatcgaa
    12061 atcctttatc cagggatatg ttcttccaag tcggcctgag ccgattgtac caagtataag
    12121 atggagcgca cgagaagaat aatgcctcgc taatcttgaa ggaataccga acaagattat
    12181 gctagaatca tgaaggaaaa atctgtcaca aagataatta tctaattttc ctaaaggaga
    12241 cactcaggat ggccaacaag aagaaaatcg aagagcgcga agcagaattg cgcgcccaac
    12301 aaacggagcg cacaatcaga atcgtaggga taggcgttgt cgcacttatc gtcttaacat
    12361 taattttcat gtatttttct aatcgcaccg acgctgtccc tacagaagta ccgctgaccg
    12421 aagtacccca aaccgaactt ccgctgacgg agccgcctca agctgaaact cctgttgaga
    12481 acaatttctc gtatgatgcc tatcctccca tgaccattga tgtaagcaag gagtattttg
    12541 cagatgtcaa aatggcagag ggcggggaat ttacgattca gcttttcgcc gataaagctc
    12601 cgaaaactgt gaacagcttt gtattccttg cccgcgcggg atatttcgat gggctgacgt
    12661 tccatcgtgt tcttcaagac ttcatggctc agggtggtga ccctactggc accgggatgg
    12721 gcggtccagg ctatgaattt gagaacgaag aaagtgattt gacatttgac aaagagggcg
    12781 tcgttgcaat ggcgaatgcc gggcccgaca ccaatggtag tcagttcttt attacctttg
    12841 gtcccacccc gcatctaaac ggtgggtata caatcttcgg gcaggtaatc gaagggatgg
    12901 atgtggtcaa ggcgatcacg ttgcgcgacc cagatgcgaa tcccacgttt actggtgacg
    12961 taatggaaag tgtaacgatc accgagcaat aagtggcaca actacgagaa gtcctcacga
    13021 gaaacctcgt gaggactttt gaattattta gcttttaaat gtattacgcg tacatgcccc
    13081 aatcgagtgc ggaggggtcc tccatcatcg aaaagtccca catatccatt cccatctcaa
    13141 ttgtgacggg catgtctagc gcttccaccg ccttggcttt ggtcatttcg atcatggctg
    13201 gcccataggg gcagaaaggg gtagtaagga tcattttcaa gcgggcaata tcattttcga
    13261 tcacgatgtc gcgcactaag cctaattgga taatgttcat accaatttcc gggtcaacca
    13321 cttctgaaag ccggtcacgc gagacctgca ctctatcggg atgtgtatcg tgaattgtcc
    13381 acttgattgg attaacagtc tcttcagtca tttttcctca taaaatttgg tttgatatgt
    13441 ttcggcaaat caatttttac tgccagcatt tacaacgtac gtacaatgag cgctgccatc
    13501 cagaatgcac tggattttgt taaccggcaa cgcaagcatt tttgaaatca aagtctgatc
    13561 aacggtgcat acctcagggt gggcaattcc aatctgataa taagggcagg aaatttcatg
    13621 gatctggtat tcgccgcctt ttttctccca ttcgaccgtg aagccttcct gcgccagcaa
    13681 ctctttgaca aactccagcc tttcttccat gctcaggccc tgtatctgac tcgaatattc
    13741 attcgcgaga tcttcggcaa tttgcccaaa caactgactc accacgggtg caggcatcgt
    13801 ctccttcatc cgagtgagca aacgcgtcgt aagacgaaga tatttggtcg ggaatttctc
    13861 cattccgtct tcagtaagcg aatacaccag gcgggggcgc ccaaccccgt gcctttcttc
    13921 atcagcccca acgaggcctt ccatctgtag gttcgtcagg tgatggcgca cagaaatcgg
    13981 attgatcccc accgcttccg ccaggtcatt gatcgtgatc tttggccttc gaagcaaagt
    14041 ctgcaaaatc ttatctttcg tgcttttcat gggcgggagt ataaccttcg ggcatggtcc
    14101 tgtcaatata ctagataaat atcataaata tccgtgaatt gtctatcaag cggaatcatc
    14161 ttgcggccag aagttaaaat caaaaggtga cgctgaaaat tcaccagcgt caccttcgag
    14221 tgcccaaaca ctactcaggc tatgggacgc cgatcctaaa cccctgatcc attgatgcag
    14281 ctgaggcgtt gttataccag gggaaccagt actcggtaga aagctgttct gccggcaacc
    14341 ccatcatttc ggaataacta gcgcggaagc caggttcctg ccagatgact cgcagcgcgg
    14401 cgatgattcg atcattagca ttgccagcat tacacgtcgt gcagacaatc cggatcggac
    14461 cgttgttgac ggaaaagccg actctcacgc tgcctccggc cgcaaggctg aggtcagtgc
    14521 ctacctgggc ggcacccaca aatactcgaa cggtgtggcc aacggtgtcc acattcgcaa
    14581 tgcggaaacc ctgatccatg gatgctgtgt cgagattgtt gtaccagggg aaccagtatt
    14641 cggtggaaag tgcttcggca ggtaagccca tcatctcaga gtaacttgcg cgaaagccgg
    14701 gttccttcca gataacgcgc agggcagcaa tgatcttatc cgtacccgtt gagcaggttg
    14761 tacaacgtat ctggattggt ccattgtcaa tagtgtaacc cacgcgcaca ctcgctcctc
    14821 caccgagcgc aattgtgtcg agtagggttg tcccaacccg cacttcgact gtgtttggtc
    14881 cagcatcgac atttgcgatc cggaatcctt gatccatgga attgaccgca gcattgttat
    14941 accaagggaa ccaatattca ctcgagagct gctctctggg caaccccatc atctcggagt
    15001 aactagctcg aaaaccgggt tctttccaga tgacacgcag ggcagcgatg atcttatcgg
    15061 tgcccgtgtt cgagcaggtg gtgcatagga tcttgattgg accattgtcc actgccgggt
    15121 agccaaccct tacactatct cctccaccaa gagcaatcgt gtcgagcagg gttgtcccaa
    15181 cccgcacttc gacagtgttg gctccagaat ctacatttgc gatgcggaat ccctggtcca
    15241 tagagtttgg agcagcgaag ttataccagg gaaaccaata ttcattggag agttgttcca
    15301 ctggaagtcc catcatctct gagtagctgt agcgcggacc tggttccttc cagatgacgc
    15361 gtaaggcggt aatcaccgat gctccgttgg tgcttacgac ttgtgccgga ccgtcatcca
    15421 caacatagcc gtctcggata ctttggtttt gtattaatcc atatttccct ttcagctgat
    15481 cgccaatgaa cacattctgt ctatcaggca cagaggtgtt tatccaactg acaaagtctg
    15541 atacccgggt ataaactcct gggttaacac cacaatcaat tccccaggag acgattcccg
    15601 ccaaagtcca tttattaccg gatggcacta cgagcggccc gcccgaatcc ccctgacagg
    15661 catccttgcc agccgatcca gcgcatagca tactggacgc gatcgtaaag ccaaatggat
    15721 tgtaggcgtt attgcaattc gtgtttgtga tcacaggaat ctgcacttca agaatccgat
    15781 ccgccagcgg accgcccgac gctatcgctc cccatcctgt tacccaggcg ttggttcccg
    15841 ccagggtgcc tactgatgat gagaccagag gaaccaaact ggtcttaatc tgaccagtat
    15901 agccaatcgt aattggactc cctaattgga gtaaggcaat atcaaattcg agattgggtg
    15961 atgtggggtt gaaagatgca tgtcgaatga tttgagatac atttcgccgc tgaaagcccg
    16021 ccacgggagt aaccacatta tagacacccg caaccacatc tatatctgca gaatttatcg
    16081 gggttggggg attggttgaa aagtcatata cacaatgtgc tgcagttacc acccactgtg
    16141 aggttattaa tgaacctcca caaaactgtc cgaggagatc cggatcagaa ccaccgatca
    16201 gagcgacctg ccacggccat tcaccggggt cagaggctgc tccaccaacg atttctggat
    16261 catttgggtc tcggactgga gtaggtgtcc aggctgcttg tgctcccact gtagaagaag
    16321 atgtgtttac gataatc
//
LOCUS       DASTJP010000873        14152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_718491, whole genome shotgun sequence.
ACCESSION   DASTJP010000873 DASTJP010000000
VERSION     DASTJP010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14152
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718491"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFQ23_25320"
     CDS             complement(<1..1051)
                     /locus_tag="VFQ23_25320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9909994.1"
                     /translation="MGKSRTDKQIGYLLRSYPRLSQTFILNEILALEKIGVAIQIFAL
                     TNPHEKVVQMQVEQVQAPVQYLEEAMQKRPWVQIVREHLKVASLHLPGYLRALSYIAT
                     HPKIDKGYTASNRWECFLQAIHLIYILTLQEQRTGKKLDHLHAHFAHDPALIAYLVNY
                     ITGLPFSFTAHARDLYQVPEDVLIDRIHQARAVVTCCGANIDYLNQIAASQKSKISLI
                     YHGVNLEDFQLAPKIGTTARPEIPIILSVGRLVEKKGFQDLFQALRMVKERGQQFRCT
                     IYGDGPLSQQLKAWIDEHGMSDEILLMGDRSQHELIPIFQGATFFALTPVQTDDGDRD
                     GIPNVLVEAMAVGLPV"
     gene            complement(973..3498)
                     /locus_tag="VFQ23_25325"
     CDS             complement(973..3498)
                     /locus_tag="VFQ23_25325"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9909995.1"
                     /translation="MEYRFDFDDQLPDLPIAFDFEEVADLFERKVFAPNAARDPVGRV
                     HVRKLQDVKYRPAERCVTTYELIVGRPDRAPERTIGVLEFTTEGVIPRLYTSDERLSW
                     LSQATDLNEMQQRFSELPALASYGDQLRLWEIFPVRYKPGLHSVMRYTVHTPEGNKMF
                     YGKSFTGNAGQLMQTITELHKVSQEVPDMPLISAPVAVWPEMEMILQAAVPNGVEFTH
                     FIYDQRYDISVRESWMRKAGVALGEFHNNATAPGETKTVFDDLQDLHEYTVIMGKVRA
                     DLATKYEEVIELINTKVSKFQEPKPVASHGAMRTDQYIIQGDRLAMIDLDGYCWANPA
                     RDLGNFFAYLCWKAIRQPEHAEFIERAGRAFLEGYLTVQSNVDKRWLSVYQAASLLKI
                     AGRRFRSLTFLEWPLVIHLIQAAFSAITEDLANLEADTITNIKGKLVAHLRTASSKTK
                     FPKVFEDKPFPALWSALNAEIMNDNLTPMLGDVCSASAQRFVHRAKLLAYKPGKRGVI
                     RYDLDQFECQRYSSVYGKLYPEPYLSERAYNVMNTLHNEVFRDAPDLDIPEPLGVIPN
                     LSMLVFIPAEGELLGDYIALRSLDGDEVVRAMELSGKWLAQLHTHKIPLEKEFKVENE
                     VDNIRDWAELVSKKYPDERKAISHIADYLIQKTAELDFSARVPIHKDFHYEHILIEDG
                     LKVFDFDEMRLGDPNFDLAHFCANFYLLAYRNEEHTAAFTDLQNRFLDAYSSVTGWQW
                     DEKFLFFYVYSCLKIAKQLCKLRGPRPWPEGDEQQAQVWLMIEQALTLLETAKTKFAQ
                     KETEVPVYEFAEIKKATRWAKAALISKSATSSAAILVSHRPLY"
     gene            complement(3546..4871)
                     /locus_tag="VFQ23_25330"
     CDS             complement(3546..4871)
                     /locus_tag="VFQ23_25330"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9909996.1"
                     /translation="MEYRFDFDDQLPDLPIAFDLKEVAELFERDVFNSLQKVEGKVNV
                     KKLQDVKYRPSERCVTTYELIVEKPDGSSERTIGVLEFTPQGVLPRLYTADDRLPWLT
                     KATDLNHMQQLFSELPGFGAEVTLLEIFPVRYAPGLRCVFRYTLQTPSGKEVFYGKSF
                     SGGAERLMNAFNELHKSSEQNPDMPSISAPVAVWPEMEMILQTAVPDGIEFTQFIYDA
                     AYEASVREAWIVKAGRALGEFHSKATTPSETKTVHDDLQDLREYTVIIDKVRPDIAVK
                     YGEVIQLILEKVGQFEEPKSMAGHGAMRTDQYILQGARLAMIDLDSYCWANPARDLGN
                     FLAYLCWKATRQPEHAEFIERAGRTFLEGYLSVQGNIDERWLSIYQAASLLKIVGRRF
                     RSLTYREWPLVIHPIRLAELFLNEVLETASGTQRPEWNLKKKLSAQQSQ"
     gene            complement(4916..6529)
                     /locus_tag="VFQ23_25335"
     CDS             complement(4916..6529)
                     /locus_tag="VFQ23_25335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9909997.1"
                     /translation="MTDSIQKDGEKSQPPTGVLLQAKDLTVRTPAGTSGLTDISFHVE
                     PGEVVALTDLGRSGKSTLLECLAGLIQPERGEVLIDGINLHTNLKAFRPIIGFVPAEF
                     ALHQNLTALETLQDAALLRLPRGTRTQDRQQRVISVINSVGLSPVADRQVGLLSNVDQ
                     RKLSIAIEMMGSPKLLLVDYPLKPLSSFEDVQITILLRELSRQGITIIQVNPRSRSTG
                     VTDKIIFLAPKGFLGWFGPADETLNYLKNYLPRGVVKDLFGLQEALEMLVNPLETDGS
                     EWAKKYKDHESYQKYVDDPLHNRYPDLLLQTRPLIRLRLRNSAGEKVPPTVIPRAGFV
                     QKLMLFIRRNFRLLWRDRAWLAMLVIPLLVAIVHLLLSPRSAPSGQPVVFDLFTFLIV
                     LTSAFLVQNEIFKEKAVFQREQRTSPILIPYIVSKLWLVALLSVYQGFVWAIIRTFGS
                     AAGSQELLSSVITLSLVAFFGGVLGLIVSALSKRTMTSISWVLLLTVPLLLSFFNPLS
                     SWLSLVIVSLLLIVLLLVIQNSAGSVRTS"
     gene            complement(6616..7773)
                     /locus_tag="VFQ23_25340"
     CDS             complement(6616..7773)
                     /locus_tag="VFQ23_25340"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9909998.1"
                     /translation="MQRTCSIAWEIYRLQGDASILTFSDSQLGQFFPVSPHHDYIKLP
                     SIAKDSPGKWKATHLSMTFPEILHLRKQLLSHVLINYSPDILLVDHMPHGAMGELLPA
                     LEALKHSRLHTQVILGLRDILDGPEVTINRWKVEGAYDAIERYYARILVFGMKDVYNV
                     VEKYQIPAAEAKKLFYCGYVTNLDNANNAHAIRARFLANKSIHTKLIVVMAGGGADAY
                     PLMRTLIDALPKVLEHHKCVVAIVTGPFMPADLIADLNRRAAKLPIHMMESVHDSLSH
                     IAAADLVISMAGYNTSVEILRTRRPAILVPRAGPSAEQRTRAKLFADKHWVDMIDPDE
                     LTAKKLVQRISYHFKHAAENHQNEPPNLNGAAAAAALTLSVLASKNERVAG"
     gene            8067..10232
                     /locus_tag="VFQ23_25345"
     CDS             8067..10232
                     /locus_tag="VFQ23_25345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9909999.1"
                     /translation="MPEWIGKTVGKVRIEREIAKGGMAEVFLGTHLTLDRPVAVKVMH
                     NYIEADPDFQSRFEREAKVVAALRHPNIIQIFDFDTADGRPYIVMEYLKGPSLASYLS
                     ELHKRGDRLPPARIARLLAALASAIDYAHEEGVIHRDIKPGNIILHNKARELSTNDPL
                     TPLTEPVVTDFGLVRIVNSATQTASGMVSGTPAYMSPEQAQGVKVDHRSDIYSLGVLL
                     YEMVAGRVPFEADTSWTVILKHINEAPPPITGIQPPVQGVIERVLAKNPDLRYQSCRD
                     LAADYLEAIGLVAQSETMRFSSQSTPRAVPADTSARATPPSAPNFNSTQDQAIPSFDK
                     RYPAASPVWMRVLPVAGLIILLLGLTVFAFSRLSQPASPTNSPGSIQTSDNGTHATSE
                     THAVLPIDNQSSLGLLRFQDGTASGDQVTISTASMPAPPQGSQYEAWLIADDGEQRVS
                     IGTLSFDPENRGTLLFVDSNGQNLIGKYHALEITIEPSPDNNPINSSDIAFSVTLPRN
                     GFTHVRHLLFSYGATPNQVSLIHGLDATTRGINLSAEEMLTAFESGDEANVRLQAEEI
                     LNAIIGNQDPNYKDWNNDGDIDDESDGFGLLLNGENEGYIQGTISHAGFAAEANDATE
                     NMRIHGQHVQIAGNNIAVWTPQLRDQLTSIISAPFDSSMEGLIRQAVSITNQIQNGID
                     INGNENIEPIEGEGGVNTAYEHAYYMADMSIFASQLPVP"
     gene            complement(10214..11140)
                     /locus_tag="VFQ23_25350"
     CDS             complement(10214..11140)
                     /locus_tag="VFQ23_25350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910000.1"
                     /translation="MKYGNFHGHKSVILENVHFQVECLAQAGPRVVRLIPAWTGENLF
                     AEVPDFTTKTTLGEYQYFGGHRLWYAPESLSRTYSPDSHGISIKEVQDGLRLTGMDEP
                     GTGIQKTLTIQISPTRSFIILQHKLQNNGRATVRLAPWAITMLRPTGVAILPQQHGNL
                     DDDGVMPNRRFALWPYSRWDDVRLQLGDEFVIIKGDNTNSSPLKLGYFNPHGWLGYLC
                     NDVFFVKRFGVRRDKEYADYGSNSEIYTNDRITELESLGPILDLNPHEDVVHTETWEV
                     YRDTDIPQDLFGGKTLQDVLSKNRNLNHGTGS"
     gene            11219..11692
                     /locus_tag="VFQ23_25355"
     CDS             11219..11692
                     /locus_tag="VFQ23_25355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9910001.1"
                     /translation="MTIKQPNSKMCFICGLENPIGLHLHIYETEPGVVESTYLAPAHF
                     QGYPGVLHGGIVAAIIDEIAGRTLMGNDPMNPRFMFTAKMEVKYRKNVPIEKELKIIG
                     KAGKSRAISAEAWAGIYDAKTNELLAEGNVLLINVPEGQFDRSRLSELGWKVYPD"
     gene            11758..12333
                     /locus_tag="VFQ23_25360"
     CDS             11758..12333
                     /locus_tag="VFQ23_25360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9910002.1"
                     /translation="MTTLMATQSKTAEDDVQLVRQSLTDANAFAELYRRNILRVYRYH
                     LANIGNVKDAEDLTSQTFMAALEGIRSFRGTGSFAAWLMGIASKKRLMHFRGSRPEVP
                     LDTALHHPSPELPTELAAIQRLQVDSVSRALRNIPRDRAEAITLSYFGGLTNSEVSRV
                     MNKSEAAVKMLIARGLQDLRERTSLILEVER"
     gene            12330..>14152
                     /locus_tag="VFQ23_25365"
     CDS             12330..>14152
                     /locus_tag="VFQ23_25365"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET9910003.1"
                     /translation="MNDKFTNKNENEEAVSGILQSVAQNIEVNTDFQTALEKQLTEAH
                     KPARISFMFSAFQRTMPTLGWIAALAFMILMLNWIIRSIAPPPVPAAQETATASQPRT
                     PSPIATVENTTPMPDAEGYTWRGSRLYLSQPLPTSPAEASVYQLRRDQQVTLEEARAL
                     ARRFGLAGDVYTTFNYAFNVDNYYFTDGKQGLEVYSNRRFIYTADLAKAGRVTQLPPV
                     NNAEQIIRDFLSARGFDFPFKITPSDFFDRYSINPLAPDGLAMQYESFTFPPMLIQLD
                     ENNQVLSIDATLLEYEPTPLGTYGIISAQEALNILMNDNEQVGKQDFFHSAANPPPQE
                     WYRQYPDNQIITIRAYSFSVPHLEAGKPPFITIDGVPAVGNIAGLAQLEPSTLIEATG
                     EFRVESGIRQFNVESWDTNITEDYVEGTLRQEGDQIILTNRDGSDREYAIIDPPADLP
                     LNTNIDTSLVAFSGVIQDGQMDWYYIQFYEDIMGSGGGGGGGGGLGFYQLNLSGTPIP
                     FPTPTTQTSITGGTYIVQENDTLASIALNFGISVEEIAQANGLPDSNLIYVGQLLVIP
                     GLEIASDQRVDNVRGFLSISIQRSADGSQTTSYLVFVPDQSS"
BASE COUNT         3720 a         3591 c         3312 g         3529 t
ORIGIN      
        1 cgacaggcag accgacggcc atcgcctcga ccaacacatt gggtatgccg tcccgatcac
       61 catcatcagt ttggacggga gtcagtgcaa aaaaagtagc accttgaaag atcgggatta
      121 actcatgctg cgaacgatcg cccatcagca gaatttcgtc agacatgcca tgttcatcga
      181 tccatgcttt taactgttgg gagagtggtc catctccgta aattgtacaa cggaattgtt
      241 gccctctctc cttaaccatt cgcagcgcct gaaataaatc ctgaaagccc ttcttctcca
      301 ccagtcgtcc gaccgaaagg ataatcggaa tctcagggcg agctgtggtg ccaatttttg
      361 gagctaattg aaaatcctcg agatttacac cgtgatagat caatgagatc ttcgacttct
      421 gtgaggcggc aatttgattc aaatagtcaa tgttcgcgcc acagcaggtg accacagccc
      481 ttgcctgatg gatgcggtct attagcacat cctcgggcac ctggtagaga tcacgtgcat
      541 gggcggtaaa gctaaacgga agtccggtta tgtaattcac gagataagca atcagggctg
      601 ggtcatgggc aaagtgtgca tgcaaatgat ctaacttttt tccggttctc tgttcttgca
      661 atgtgagaat gtaaatcaag tgaatcgcct gaaggaagca ctcccagcga ttgctggcgg
      721 tataaccttt atcaatcttt ggatgggttg caatatagga taacgcgcga agatatccag
      781 gaaggtgtag actggcgacc ttcagatgtt cgcgtacaat ctgcacccat ggccgcttct
      841 gcatcgcttc ctccaggtat tgaactggag cctgaacttg ttccacttgc atttgaacaa
      901 ccttttcatg agggttggtc agggcaaaga tctgtatcgc tacaccgatc ttttccaaag
      961 caaggatctc gttcagtata aaggtctgtg agagacgagg atagctgcgg aggaggtagc
     1021 cgatttgctt atcagtgcgg cttttgccca tcgagtggcc ttcttaattt ctgcaaactc
     1081 atatacgggt acttcggttt ctttttgcgc aaattttgtt ttcgcggtct ccagtagcgt
     1141 gagcgcctgt tcgatcatta accagacctg tgcctgctgt tcatctcctt ctggccaggg
     1201 acgcggaccg cgcaacttgc ataactgctt ggcaattttg aggcaggagt agacgtagaa
     1261 gaacaggaac ttctcgtccc attgccaccc cgtcacgctt gaataggcat ccaaaaagcg
     1321 attttggagg tctgtaaaag cagcggtatg ttcctcattg cggtaagcaa gcagataaaa
     1381 attggcacaa aaatgtgcga ggtcaaaatt tgggtcaccc aggcgcatct cgtcaaaatc
     1441 aaataccttc aagccatcct cgatcagaat atgctcatag tgaaaatctt tgtgaatggg
     1501 gacacgcgcg ctaaagtcaa gctcggctgt cttttggatc agataatctg caatatgact
     1561 aattgccttt cgctcatccg gatatttctt tgagacgagt tctgcccaat cacgaatgtt
     1621 atctacttca ttctccacct tgaactcttt ctcgagcggg attttatgcg tgtgcagttg
     1681 cgccaaccat tttccagaaa gctccattgc acgaacaacc tcatctccgt ccaaggagcg
     1741 caaggcgatg taatccccca acaactcacc ttctgccgga ataaaaacca gcatggaaag
     1801 gtttgggatc acacccaacg gttcaggtat atccaggtcc ggagcatccc ggaacacttc
     1861 attatgaagc gtattcatca cattgtatgc tcgttcagat aaatatggct caggataaag
     1921 tttgccatag acagaagaat atctctgaca ctcgaactgg tccaggtcat aacgaatgac
     1981 accgcgtttc ccaggcttat acgccaataa cttagcgcgg tgcacgaacc gctgagcaga
     2041 agcagaacaa acatcaccaa gcataggagt aagattgtca ttcatgatct cggcattaag
     2101 cgcgctccat agggcaggga atggtttgtc ttcaaatact tttggaaatt tcgttttact
     2161 gctggctgtc cgcaaatgag ccaccaactt ccccttaata ttggtgattg tgtctgcttc
     2221 aaggtttgct aagtcctccg taatagcaga aaaggcggct tggatcaggt gaatgacgag
     2281 cggccattcc aaaaacgtca gacttcggaa gcgacggccg gcaattttca gtagagaggc
     2341 ggcctggtag acggaaagcc atcttttgtc aacattgctc tgcacagtca ggtatccttc
     2401 gaggaacgcc cgccccgcac gctcgatgaa ctcagcatgt tcaggttggc gaatggcctt
     2461 ccagcacaag tacgcaaaga aatttccaag atcacgagcc ggattcgccc aacagtagcc
     2521 atccaggtcg atcatcgcca gacgatcccc ctggatgatg tattgatccg tccgcattgc
     2581 gccatggctt gccacgggct tcggttcctg aaacttactg actttggtgt tgatgagttc
     2641 gatgacctct tcatattttg ttgccaggtc ggctcgaacc ttgcccatga tcaccgtgta
     2701 ttcatgcaga tcttgcaggt catcaaagac tgttttcgtt tcgccgggcg cagtcgcatt
     2761 attgtggaat tcccccaatg caacacctgc ttttctcatc caactttcgc gcacagaaat
     2821 atcatagcgt tgatcgtaaa taaaatgtgt aaattcaacc ccgtttggaa ccgcggcttg
     2881 taagatcatt tccatttcag gccacacggc aacgggcgca gaaatcagcg gcatatccgg
     2941 aacttcctgg ctcactttgt gaagctcggt gatggtttgc atcaactgtc ccgcattgcc
     3001 cgtaaacgac tttccataga acattttatt cccctcaggc gtgtgcacgg tataacgcat
     3061 cacactgtgc aagccaggct tataccgaac cgggaagatt tcccatagcc ttagctgatc
     3121 gccatagctc gccaaagctg gcagttcaga aaagcgttgt tgcatttcgt tcaagtcagt
     3181 tgcctgagat aaccaagaca agcgctcgtc ggaggtgtaa agccggggga tcactccttc
     3241 tgtcgtaaat tcgagaaccc caatagtccg ctctggagca cgatctggtc ttccaacaat
     3301 cagttcatag gtagtaacgc aacgctcggc aggtctgtat ttgacatcct gtaatttccg
     3361 gacgtgcacg cgacccaccg gatcacgagc agcattcgga gcaaacacct tgcgttcgaa
     3421 caggtctgcc acctcctcaa aatcaaaggc gatgggcagg tctggtaatt gatcgtcaaa
     3481 atcgaatcga tattccatac ttaaaatcct aatgatccat gatgcaaact agatgaatat
     3541 gtgacttatt gtgattgctg tgccgacagt ttctttttga gattccactc cggtctttgc
     3601 gtacctgacg ctgtttcgag cacttcattc aaaaacagct cagccagcct gatcggatga
     3661 ataaccagag gccattcgcg ataggtcagg cttcgaaaac ggcgaccaac aatttttagt
     3721 aaggaagccg cctgataaat ggataaccag cgttcatcga tatttccttg aacactcagg
     3781 tatccttcaa ggaacgtgcg acccgcacgc tcgataaact cagcatgttc aggttggcgt
     3841 gtcgctttcc agcacaagta cgccaggaaa tttccaaggt cacgagccgg gttcgcccaa
     3901 caatagctat ccaggtcgat catcgctaag cgagccccct gcaggatata ttgatctgtg
     3961 cgcattgcgc cgtgacctgc catagacttc ggttcttcaa actggccgac cttttcgagg
     4021 atgagttgaa tgacttctcc atacttaaca gcaatatccg gtcgtacctt gtcaatgatc
     4081 accgtatatt cacgcaggtc ctgcaggtca tcatggacgg ttttcgtttc acttggagtg
     4141 gtcgctttgc tgtggaactc cccgagagca cgcccggctt tcacaatcca ggcttcccga
     4201 actgaagcct catatgccgc atcgtaaata aattgagtaa actcaatccc gtctggaaca
     4261 gcagtttgta gaatcatttc catttcaggc cagacggcaa ccggcgcaga aatggatggc
     4321 atgtccggat tttgttcact ggatttgtga agctcattaa acgcgttcat gagccgttct
     4381 gcgccaccgg agaacgattt tccataaaac acttctttgc cggaaggcgt ttgcaaagtg
     4441 tagcggaata cgcagcgcag cccgggtgca taacgaaccg ggaagatttc aagcaatgtg
     4501 acttctgctc caaaacctgg caactcagaa aagagttgct gcatatgatt cagatcagtg
     4561 gcttttgtca accaggggag gcgatcgtcg gcagtgtaaa ggcggggtag aacaccttga
     4621 ggggtaaatt ccaaaacgcc aatcgttctc tctgaagagc catccggctt ttcaacaatc
     4681 aattcgtagg tggttacaca acgctcggag ggtctgtatt ttacatcctg caacttcttg
     4741 acatttactt tgccctcaac cttctgcaaa gaattaaaca catcgcgctc gaaaagttct
     4801 gccacctcct tcaaatcaaa cgcgatgggc aggtctggta attgatcgtc aaaatcaaat
     4861 cggtattcca tacttaaaat cctaatgaca ggtcattcaa agcgaccctg gcatctcacg
     4921 aggttctaac gctccctgcg ctgttttgga tcaccagtaa aagaacaatc aacaacaggc
     4981 tgacaatcac caaactcaac cagctactta atggattgaa aaaagaaagc aacaacggaa
     5041 cggtaagcaa gagtacccag ctgatggacg tcatcgtcct tttcgataac gctgaaacga
     5101 tcaatcctaa tactccaccg aaaaacgcaa ccagcgataa cgtgatcacc gaagaaagaa
     5161 gctcctggct tcctgcagca ctcccgaacg tacgaatgat cgcccaaacg aatccctgat
     5221 agactgataa aagcgcgacc agccagagtt tggatacgat gtaaggaatt aatatagggc
     5281 tggttcgttg ctcgcgctga aatacagcct tttctttgaa gatttcattc tggaccagga
     5341 atgcagatgt caacacaatg agaaaagtaa atagatcaaa tacaactggt tggcctgaag
     5401 gcgcgctgcg gggagaaagt aataagtgca cgatcgcaac aagaaggggg attaccaaca
     5461 ttgccagcca cgctcgatct cgccagagca acctgaagtt ccgccgaatg aacagcatta
     5521 gcttctgtac aaaacccgcg cgagggataa cggtaggcgg caccttttca cccgcgctat
     5581 ttctcaatcg caggcggatc agcgggcggg tttgcagcag gagatctggg tatcgattat
     5641 gaagaggatc atccacatac ttttgatagg actcatgatc tttgtatttc tttgcccatt
     5701 ccgacccatc ggtttccagc gggttaacca gcatctcaag cgcttcctgc aatccaaata
     5761 aatctttaac aactccccgc ggaagatagt tctttaggta atttagtgtt tcatcagccg
     5821 ggccaaacca acccagaaaa cctttcggcg caaggaaaat gatcttatcg gtaacacccg
     5881 tgcttctgga gcgcggattg acctgaataa ttgtgatgcc ctgtcgggat aattctcgca
     5941 atagaatggt aatttgtaca tcttcaaaag aagaaagcgg ttttaaggga taatccacca
     6001 gaagtaactt cggactaccc atcatttcga tcgcaatgct caactttcgc tgatccacat
     6061 tgctcaacaa tccgacctgt cgatctgcaa ccggagataa tccgacagaa tttatgacgc
     6121 ttatcacccg ttgctgacga tcctgagtgc gcgtgccgcg cggcaggcgt aatagtgccg
     6181 catcttgaag tgtttctaac gccgtgagat tttggtgtag cgcaaattct gcgggtacaa
     6241 atccgatgat cggacgaaaa gctttcaggt tcgtatgaag attaatacca tcaatcagga
     6301 cctcgccgcg ttcaggttgg atcagtcccg caaggcattc taacagcgtg gatttgcctg
     6361 aacgacccag atccgttaac gcaacaactt cacccggctc aacatggaaa gatatatctg
     6421 taagtccact ggttcccgca ggagtgcgaa ccgtcaggtc ctttgcctgg agcaggactc
     6481 cagttggcgg ctgggacttt tcgccatcct tctgaatcga atcagtcata acactcttaa
     6541 ttccttctgc catgggtgaa tttttgttcc gcaactcgca tcatttgctt ttatgattta
     6601 aggcaaacaa acgagttagc cggcaacgcg ctcgttttta gaagccagca ccgaaagggt
     6661 cagcgcagcc gcggccgccg ctccattgag attcgggggt tcgttctgat ggttctctgc
     6721 cgcatgctta aagtgatagg aaattctctg aaccagcttc tttgcagtaa gctcatccgg
     6781 atcgatcata tccacccaat gtttatccgc aaataacttg gcgcgcgtgc gctgttcggc
     6841 gctggggccc gcacgcggga ccagaattgc aggccttcgc gtacgcagaa tttcaacgct
     6901 ggtgttatag ccggccatcg agatgacgag atcagcagct gcaatgtgac tcaaggaatc
     6961 atgtacagat tccatcatat gaatcggaag tttggccgca cgccggttaa gatcggcaat
     7021 taagtcggca ggcataaagg ggccggtgac gatggctaca acacatttgt gatgttccag
     7081 gactttcgga agcgcatcga ttaatgttct catcaatgga taagcatctg cgccaccacc
     7141 agccatgacg acgatgagct tggtgtggat ggatttattg gcaaggaagc gagcgcgaat
     7201 ggcatgcgca ttattggcat tatcaaggtt agttacgtaa ccacaataga ataatttttt
     7261 cgcttctgct gcggggatct gatatttttc gacgacgtta taaacatcct tcattccaaa
     7321 taccaaaatc cgcgcataat agcgttcaat ggcgtcgtag gccccttcta ccttccagcg
     7381 attaatcgtt acctcaggac catctagaat gtcccgcaat cccaatatga cctgggtatg
     7441 caatcgagaa tgctttaagg cttccagggc tggtaaaagc tctcccatcg ccccatgcgg
     7501 catatggtct accagaagga tatctggtga ataattgatc agcacatggc tgagcagttg
     7561 tttgcgaagg tgaaggatct cgggaaacgt catactcagg tgtgtcgcct tccacttccc
     7621 ggggctgtcc tttgcaatcg aagggagctt tatataatca tgatgagggg aaaccggaaa
     7681 aaactgaccc aattgtgagt cagaaaaggt caggatcgaa gcatcccctt gaagccgata
     7741 gatttcccat gcgatggaac aggtgcgctg catgtggccc aagccaaaac catcctgtga
     7801 atagatggca attcgtagac cctcaggtgt cctggtttct attgtgatct cctatctggg
     7861 attggcgaac gctttgccct aactcataat agcagaagag atttcgatgt gctgggttat
     7921 gattttgtag tcaagataga ttttaattgt attttaacaa accttgtttg tcaacaaact
     7981 gtttttcttg acggacgaga gttcaaaaat tattctcata ccgtttcaaa tcgtggcaca
     8041 aagatatcgt gttgtataat tctttcatgc ccgagtggat tggaaagacg gtcggcaagg
     8101 ttcggattga gcgagagatc gcaaaagggg gtatggcaga ggtctttctt ggcacacacc
     8161 tcacattaga tcgtcccgta gcggtcaagg taatgcataa ctacatcgaa gcagacccgg
     8221 acttccaatc gcgtttcgaa cgcgaggcaa aggtcgtcgc cgcattacgc catccaaata
     8281 ttattcagat cttcgacttt gacactgctg atgggcgccc ctacattgtg atggagtatc
     8341 taaaaggtcc atcactcgct tcttatttaa gtgaactgca caaacgaggg gatagattac
     8401 ctccagctcg aatcgctcgc cttttagcgg cccttgcatc tgcgattgat tatgcacacg
     8461 aagaaggtgt catccatcgc gatatcaaac cgggaaatat cattcttcac aacaaagctc
     8521 gcgagctttc aaccaacgac cctctaactc cgctgacaga acctgttgtg accgactttg
     8581 ggttggtgcg tatcgtaaat tcggcgactc aaaccgcgtc ggggatggtc agcggcactc
     8641 ctgcgtatat gtcaccggag caggcgcaag gcgtgaaagt agatcatcga agtgatatct
     8701 attctctggg tgtgctccta tatgaaatgg tcgcaggtcg cgttcccttc gaggcagata
     8761 cttcctggac tgtgatcctc aaacacatca acgaggctcc cccgccgatt acaggaatac
     8821 aacctccggt gcagggcgtg atcgaacgcg tgttggcgaa aaatccggac ctgagatatc
     8881 aatcctgtcg tgacctggct gcagattatc tcgaggcaat cgggctggtt gcccaatccg
     8941 agacaatgcg tttctcatct caatccacac cgcgggcggt ccccgccgat acctccgctc
     9001 gagcgacgcc tccttctgct ccaaatttca actccaccca ggaccaggcc attccttcct
     9061 tcgataaacg gtatccagcg gcaagtccgg tgtggatgcg ggttttgcct gtagctggtc
     9121 ttatcatctt attacttggt ctcacagtat ttgctttttc aaggctttca caaccagcgt
     9181 ccccaacgaa cagccccggg tcaatacaga cttcggacaa cggcacgcat gcaacctcag
     9241 agactcatgc agtgctgccg attgacaatc aatcttcgct tggcttgctg cgattccagg
     9301 atggaacagc aagtggtgat caggttacga tttccacagc ctccatgccc gccccacctc
     9361 agggcagcca atatgaagcc tggctgattg ccgatgacgg tgagcaacgc gtcagcattg
     9421 gaaccctttc ctttgacccg gaaaatcgag gaactcttct ttttgtggat agtaacggac
     9481 aaaatttgat cggcaaatat catgccctgg aaatcaccat cgaaccaagc ccggacaaca
     9541 acccgatcaa ttccagcgat attgctttct ctgttacctt accgcgcaac ggtttcaccc
     9601 acgttaggca tctgcttttt tcttatggcg ccacacccaa tcaggtcagt cttattcacg
     9661 gattggatgc cacgaccagg ggcatcaatc tatctgctga agagatgctt accgcgtttg
     9721 aatcaggtga tgaagccaac gtccgtctcc aggcggaaga gatcctaaac gccatcatcg
     9781 gaaatcaaga cccgaattac aaagactgga ataacgatgg ggatattgat gatgagagcg
     9841 atggcttcgg actcttattg aacggagaaa acgaaggcta tatacagggc acgatctctc
     9901 atgccggctt tgctgcggaa gcaaacgacg ccacggagaa catgcgcata catggccagc
     9961 atgtacaaat cgctgggaat aatatcgctg tatggactcc tcaactacgc gatcaattga
    10021 catccatcat cagcgcacca tttgactcaa gcatggaagg gctgatccgc caggctgttt
    10081 caatcacaaa ccagatccaa aatgggatcg atatcaacgg gaatgaaaat atagaaccaa
    10141 ttgaagggga aggcggggta aatacagcat atgaacatgc ctattacatg gcagatatgt
    10201 cgatcttcgc aagtcaacta cctgtgccat gatttaggtt tctattctta cttaagacat
    10261 cctgcagagt ctttccccca aataaatctt gcggaatatc agtgtcacga tagacttccc
    10321 aggtctctgt gtgaactacg tcttcatgag ggttcagatc caggatggga ccaagactct
    10381 ccagttcagt gatcctgtca ttggtataga tttccgagtt tgacccgtaa tctgcatatt
    10441 ctttatcacg ccgcaccccg aatcgtttta caaaaaaaac atcattacaa aggtaaccca
    10501 gccatccgtg tggattgaaa tatcctaact ttaacgggct ggaattggtg ttgtcacctt
    10561 tgatgatgac aaattcatcc cctaattgca aacgaacgtc atcccagcgg gaatacggcc
    10621 aaagcgcaaa tcggcgattc ggcatcacgc catcgtcatc caggttaccg tgttgctgag
    10681 gcagaatggc aacacccgtg ggtcgcaaca tggtaattgc ccagggtgcc aatctaaccg
    10741 ttgcccgacc attattttgt agcttgtgtt gcaaaataat aaaggatcgc gtagggctga
    10801 tttgaatcgt gagggtcttc tgaatgcctg tgcccggctc atccatgccc gtcaggcgaa
    10861 gtccatcctg cacttctttg atagaaatgc cgtgactatc cggggaatac gtacgtgaca
    10921 acgactctgg cgcataccag agccgatgcc caccaaaata ctgatactcg cccaacgtag
    10981 tttttgttgt aaagtctggt acttccgcga agagattttc ccccgtccat gcaggaatca
    11041 aacgtacaac acgaggcccc gcctgcgcaa gacattccac ctgaaaatgt acgttttcca
    11101 aaatcacaga cttgtgtccg tggaaatttc catacttcat ggtaggcgca atcttagccc
    11161 taaaacaagt ttccgtaaat gtgttttttc aaccaacatc atggaatata atcttcatat
    11221 gaccattaaa caacccaatt caaaaatgtg cttcatttgc gggcttgaaa accccatcgg
    11281 tctgcatctc catatatatg aaacggagcc gggggtcgtt gaatctacct atctagcgcc
    11341 agctcacttc cagggatacc cgggtgtttt acacggcggg attgtggcag caattattga
    11401 tgaaatcgcg ggtcgcacct taatgggaaa cgatcccatg aatccacgat ttatgtttac
    11461 ggcaaaaatg gaagtcaaat atcgcaaaaa tgttccgatt gagaaagaac tgaaaatcat
    11521 cggcaaagca gggaaatcac gggcaatatc ggccgaagcc tgggccggca tctatgatgc
    11581 taagacaaat gagttactgg cagaggggaa tgtattgtta atcaacgttc ccgagggtca
    11641 atttgaccgg tcaaggttga gtgaactggg gtggaaggtt tatcctgact gagtattccc
    11701 tgttactttt ttttcacctc ctgcaacata ttaacgtgcg cataatttag gaggtgcatg
    11761 actacgctta tggcaaccca atccaaaacg gcagaggatg acgtgcaact ggtacgccag
    11821 tcgctcaccg atgcgaacgc ctttgctgaa ctatacagac gcaacatcct gcgcgtctac
    11881 cgctaccatc ttgccaatat cggcaatgtg aaagatgccg aagacctgac gtcgcaaacc
    11941 tttatggcgg cactggaagg gattcgctcc tttcgaggaa caggctcttt cgccgcctgg
    12001 ctgatgggga tcgcatccaa gaaaaggtta atgcattttc gtgggagcag gcctgaggtc
    12061 ccgttggaca cggctcttca tcacccgagc ccagagctac ccactgagct ggcagcaatt
    12121 cagcgcttac aggttgactc agtttcacgc gctctccgga atattcctcg agaccgcgca
    12181 gaagcaatca cattatccta ctttggcggg ctcaccaatt ctgaagtcag ccgtgtgatg
    12241 aacaagagtg aagccgctgt gaagatgctc atcgcacgtg ggcttcagga cttgcgtgag
    12301 cggacctccc tcatcctgga ggtggaacga tgaacgataa atttacaaat aaaaatgaaa
    12361 acgaagaagc agtttctgga attcttcagt cagttgcaca aaatattgag gttaatacag
    12421 acttccaaac agcattagag aagcaactga cagaagccca taaacccgct cgcattagct
    12481 ttatgttttc cgcctttcag cggacgatgc cgacacttgg ttggatcgcc gccctggcat
    12541 tcatgatctt aatgctcaat tggatcattc gatcgatcgc ccctccgccc gttccagcgg
    12601 cgcaggagac tgcaaccgct tctcaaccgc ggaccccttc tcctatcgcc acggttgaaa
    12661 atacaacgcc gatgccagat gccgaaggat atacctggcg cggctcgcga ctctatcttt
    12721 cacaaccttt accgacatca cccgccgagg caagtgtata tcagttgaga agggatcaac
    12781 aggtcacact cgaggaggcg cgcgccttag cccgtcgatt tggtctggca ggtgacgtat
    12841 ataccacctt caactatgca ttcaacgttg ataactatta tttcaccgat ggcaaacagg
    12901 gactcgaggt gtattccaat cgaaggttca tatacacggc tgacctcgcc aaagccggaa
    12961 gggtaactca actccctccc gtcaacaatg ctgagcaaat catccgtgat tttctgagcg
    13021 cgcgcggatt tgattttcca ttcaagatta caccgtctga tttctttgat agatacagca
    13081 tcaatcctct ggcgcccgat ggtctagcca tgcagtatga atcctttacc tttccgccga
    13141 tgctcattca attggacgaa aacaatcagg tactaagcat tgacgcaacc ttgctggagt
    13201 atgagcctac gcctctgggt acctatggaa tcatctctgc tcaggaagcc ctgaatattc
    13261 taatgaacga caacgaacag gttggcaagc aggatttctt tcactccgct gcgaacccac
    13321 ctccacagga gtggtatcgc caatacccgg ataaccaaat tattactatc cgagcatatt
    13381 cgttttccgt cccccacctg gaggccggca agccaccatt catcaccatt gatggtgttc
    13441 ccgctgttgg taatatcgct ggtctggcgc aactagaacc atccacactg atcgaggcta
    13501 caggtgaatt tcgtgtcgaa tctggaattc gtcaattcaa cgtggaatcc tgggatacaa
    13561 atatcacaga ggattatgtg gaaggtacgc tacggcaaga gggagatcag atcatcctca
    13621 caaaccggga tggaagtgac agagaatatg caattatcga tccgcctgcg gacctgccct
    13681 taaatacaaa cattgatact tcactggtcg ctttcagtgg tgtgattcag gacggtcaga
    13741 tggattggta ctacatccag ttctatgaag acattatggg ctccggcggc ggaggcggtg
    13801 gtggtggtgg gcttggcttt tatcaactga atttgagcgg tacgccgatc ccgtttccaa
    13861 ccccaacaac tcaaacaagt attacaggcg gcacttatat tgtccaggaa aatgacactc
    13921 tggcatccat cgcattgaac tttggcatca gtgtagagga aattgcacag gcaaacggat
    13981 tgcccgattc aaacctgata tacgtcggac aactgctcgt cattcctgga ttggaaattg
    14041 cctccgacca gcgcgtcgat aacgtgcgcg gctttctctc catctcgatt caaagatctg
    14101 ccgatggctc acaaacaaca tcgtatctgg tttttgtgcc tgatcaatcc tc
//
LOCUS       DASTJP010000874         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1005147, whole genome shotgun sequence.
ACCESSION   DASTJP010000874 DASTJP010000000
VERSION     DASTJP010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005147"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFQ23_25370"
     CDS             complement(<1..911)
                     /locus_tag="VFQ23_25370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9910004.1"
                     /translation="MRYLFVVDGRSPIAINWIRHFTERDDEVYIASTFACVVDFPING
                     LEITPVAFSGLKKSGQRPGSSSSRTLSLRTALRHWLGPLTIRQASKKLRAFIERTKPD
                     LVHAMRIPYEGMLASAAINGTLYKVPFIVSCWGNDFTLHARSSRLMSQYTRRTLQTVE
                     AFHADCHRDIRLASEWGLDGSKPTLVAPGNGGLRTDIFHPAPQPVREPVIINPRGVRP
                     YVRNDNFFKAIPLVLEKHPEARFICAAMAGESQAIQWIKQLDIANAVELLPPLPHAEM
                     AKAFHRAQIVVSPSIHDGTPNSLLEGMA"
     gene            968..1363
                     /locus_tag="VFQ23_25375"
     CDS             968..1363
                     /locus_tag="VFQ23_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9910005.1"
                     /translation="MSKGRILVVEDNMDNYELVRFILERAGYDVFLAVNGRDGVDAAR
                     LQQPDMILMDLGMPEMDGWSAAEKIKSDPATRAIPLYALSAYTLPSDRKRAMDAGCDG
                     YLTKPIHMQSFLDAINEVFQAKIHEQPQS"
     gene            1485..2780
                     /locus_tag="VFQ23_25380"
     CDS             1485..2780
                     /locus_tag="VFQ23_25380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET9910006.1"
                     /translation="MKQICVVGVGYVGLVTASCFADLGNSVCALDVDEKRIENLKKGI
                     MPIYEPGLEELVKRNVKSGRLSFTNSYEEALSGAEYAFIAVGTPSGVDGNADLKYVES
                     AAKSIAQNMTAPLIIINKSTVPIGTGDWVADIVKAAQPQPIEFAVVSCPEFLREGSAI
                     ADFMSPHRNVIGSLDRDAANKVAQLHLPLRAPIVITDLRTAEMIKYASNAFLAAKISF
                     INELADICEAYGADVKEVAAGMGYDARIGRHFLDAGLGWGGSCFPKDVQALAYMAKDK
                     GLNPRMLNDTVNVNYDRRKAVAKHVETLLGGDLKDKTIGMLGLAFKENTDDMRDAPSV
                     DISNELIAAGAKVRGYDPVARKSAAPLMPEVEIFDDVYKMAEGCDALIVVTPWNEFKQ
                     LDLDRLKELLNSPVIYDGRNIYDPKYMKSIGFIYRAIGR"
     gene            2998..3900
                     /locus_tag="VFQ23_25385"
     CDS             2998..3900
                     /locus_tag="VFQ23_25385"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9910007.1"
                     /translation="MTNPPVCDYEGSDYQTSFWDKGGREYEDQTEAIALKRLLPENGR
                     LLLELGAGAGRNTPRYTGFDRIVLLDYSRTQLEQAQQRLGNSDKYVYVAGDAYRLPFV
                     DGLFDAATMIRTLHHMADAPRALGQVKNVLTAGGIFILEYANKLNLKAIFRFLFGKQE
                     WNPFALEPVEFVKLNFDFHPQAIRNWLEGLGLRIEKTLALSYFRVGLLKRIVPTGILV
                     FLDSLLQRTGTLWQYSPSVFVRAKAVEGEHRPLRDGSILSYFKCPDCGHSPLAEKSTF
                     LECTNCKKKWEIKAGIYDFREPMR"
BASE COUNT         1018 a          908 c         1070 g         1035 t
ORIGIN      
        1 gccatgcctt ccagtaatga gtttggtgtg ccatcatgga tactgggcga cactacgatc
       61 tgtgctcgat gaaaggcttt cgccatttcg gcatgcggga gtggtgggag caattccact
      121 gcattagcaa tatccaattg tttaatccat tgaatggctt gtgattcgcc agccattgca
      181 gcacaaataa accttgcttc ggggtgtttt tccaaaacca gtggtattgc tttaaaaaaa
      241 ttatcatttc tgacatacgg acgaacaccc cttggattga tgatgacagg ctctctcaca
      301 ggctgtgggg ctggatgaaa aatatcagtt cttaggcctc cattgcctgg cgcaaccagc
      361 gttggctttg atccatccaa tccccattcg ctggcaagtc gaatgtcgcg gtgacaatcg
      421 gcatgaaagg cttcgacggt ctgcagtgtc cggcgcgtat actgactcat taagcgagat
      481 gagcgcgcat gcaaggtaaa gtcgttgccc caacaggaaa cgatgaaagg aactttatat
      541 agagttccat ttattgccgc ggaggcaagc ataccttcgt acgggatccg catagcatgg
      601 acgagatctg gcttagttcg ttcgatgaat gcacgcaatt ttttggatgc ttgacgaatc
      661 gttagcggac ccaaccaatg tcgaagggcg gtgcgcaggc tcagggtcct tgaggatgag
      721 ctgcccggtc gctgacctga ctttttcaat ccactaaaag ctacaggagt gatttcaaga
      781 ccattgatgg gaaaatccac tacgcaggca aatgtggatg cgatgtagac ctcatcgtcc
      841 ctttcagtaa aatgacggat ccaattaatt gcaatggggg agcgaccatc cactacgaaa
      901 aggtagcgca tgaaatggat tataagtcat tgcccgatgc aatgagacgc ggtatagtta
      961 taccagtatg agcaagggcc gcatattggt cgtcgaagac aacatggata actacgaact
     1021 cgtgcgtttt attctcgagc gcgcaggcta cgatgttttt ttggcggtca atgggcgaga
     1081 tggtgtggat gcggctcgtt tgcagcagcc tgatatgatc ctgatggacc tgggaatgcc
     1141 agaaatggat gggtggagtg ccgctgaaaa aataaaatca gacccggcca ccagggcgat
     1201 tccgttgtat gcactttctg cctataccct gcccagtgac cgtaagcggg ctatggatgc
     1261 tggttgtgat ggctatctga caaagccaat tcatatgcag agtttcctgg atgccattaa
     1321 cgaagtgttt caggcaaaga ttcacgaaca gcctcaaagc taatttcccc aaaaactaag
     1381 ccaaatctgt aacctacttc attcgtttgg gtcatgaagt ggatagtcgt tttctcgtag
     1441 tgggcggctg tatcacaatt atctaaatct aaagaggtta aaaaatgaag caaatctgtg
     1501 ttgtcggcgt tggatacgtc ggcttagtga ccgcctcctg ttttgctgat ctgggaaaca
     1561 gtgtctgtgc gctcgatgtg gatgaaaaac gcatcgagaa tctaaagaag gggatcatgc
     1621 caatttatga gcccggtctg gaagaactcg taaaacgaaa cgtaaagagt gggcggttat
     1681 cctttaccaa ttcctatgaa gaagcactga gtggcgccga gtatgcattt attgcggtag
     1741 gaacaccctc gggtgtggat ggaaatgccg acctaaaata tgttgaatcc gcggcgaaat
     1801 ccatcgccca aaatatgact gcgccattga tcattatcaa caaatccaca gttccgatcg
     1861 ggaccggaga ctgggttgcc gatattgtga aagcggcgca gccccagccg attgagttcg
     1921 ctgtagtttc gtgccctgag tttctgcgtg aagggtcggc gatcgctgat ttcatgagtc
     1981 cgcaccgaaa tgtgattgga tcgctggatc gggacgcggc gaataaggtg gctcaacttc
     2041 atttgccgct acgtgcgccc attgttatca ctgatctgcg caccgcggaa atgatcaaat
     2101 atgccagcaa tgcatttctg gcagccaaaa tttcgttcat caatgaactg gctgatatct
     2161 gtgaagccta tggagcagat gtgaaggaag tggcagcagg tatgggttat gatgcccgca
     2221 ttggtcgtca ctttttggat gcgggtctgg gctggggcgg ctcctgcttc cctaaggatg
     2281 tgcaggcgct ggcttatatg gcaaaggaca aggggctgaa tccaagaatg cttaatgata
     2341 cggtcaatgt caactacgac cgccgcaaag cggttgcgaa gcatgtagaa acgttgctgg
     2401 gtggcgactt gaaggacaaa acgatcggta tgcttgggct ggctttcaaa gagaacaccg
     2461 acgatatgcg cgatgcaccc tctgtggata tatccaatga actgattgct gcaggtgcaa
     2521 aagtccgcgg ctatgatcct gtagcacgta agtcagcggc tcccttaatg cctgaggtgg
     2581 aaattttcga tgatgtgtat aagatggcag aaggttgcga cgcactgatc gttgtcacac
     2641 cctggaacga gttcaagcaa ctcgacctgg atcgattaaa ggaattgctc aactcgccgg
     2701 tcatctatga tggtcgaaac atttacgacc cgaaatacat gaaatccata ggattcattt
     2761 accgagccat tggtcgttag tccactggtt tcatagggag tggctaaagg gatgggctat
     2821 agtaggattc gccacagaga ccactgagtt ctttgagaaa attctctcag gtctctgtga
     2881 tctcggtggc taagcccacg aaattagccc atctcttttc atagccagta ctccacgagt
     2941 cggatggtcg ggtacaatag ccccgctatc cgacttttta ttttgactgc gagacaaatg
     3001 accaatccac ccgtttgtga ttatgaaggc tctgattatc aaacctcctt ttgggacaag
     3061 ggtgggcgag agtacgaaga ccaaacagaa gcgatcgccc tcaaacgtct tttgcccgaa
     3121 aacggacgtc ttttactgga gcttggggct ggagccggga ggaatacgcc gcgctacacg
     3181 ggttttgatc gcatcgtgct tctcgattat tcgcgcacgc agttggaaca ggcgcagcag
     3241 cgactcggaa actctgataa atatgtctac gttgccgggg atgcctatcg ccttccattt
     3301 gtcgacggtt tgttcgacgc cgcgacaatg atccgcaccc tacatcacat ggcggatgct
     3361 cccagggcgc tggggcaagt gaaaaatgtg ctgacggctg gagggatatt tattctggaa
     3421 tatgccaaca agttgaatct caaagcaatt tttcgttttc tatttggaaa gcaggaatgg
     3481 aatccattcg ccttagagcc agtcgagttt gtgaagctca attttgattt tcatcctcag
     3541 gcgatccgta attggcttga aggactaggc ctcaggatcg aaaagactct cgcactttcc
     3601 tactttcgag ttggtctcct caagcgcatt gttccgacag gtattctcgt tttccttgac
     3661 tcattactgc agcggacagg cacgttgtgg caatattcac cctctgtgtt tgtgagggca
     3721 aaagctgtag aaggggagca caggccgctt cgggatggga gtatcttgtc ctatttcaaa
     3781 tgccccgatt gtggacattc accacttgca gagaagagta cttttcttga atgtacaaat
     3841 tgtaaaaaga agtgggaaat taaggccgga atttacgact tccgagagcc aatgcgatag
     3901 acacggactt cctacggctc tgattcgttc acacttgtta cgatggatgg tggtcggata
     3961 ctgatggcgc ggtgtgtagg tatgatcggt tgcccaattg cagttgcata tacttgcgca
     4021 aattttgccc c
//
LOCUS       DASTJP010000875         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1459514, whole genome shotgun sequence.
ACCESSION   DASTJP010000875 DASTJP010000000
VERSION     DASTJP010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459514"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..619)
                     /locus_tag="VFQ23_25390"
     CDS             complement(197..619)
                     /locus_tag="VFQ23_25390"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET9910008.1"
                     /translation="MWFNPMTIWLLKSPLHGFVSKGVMLISMTGRKSGKSISTPTNYL
                     CSGNTLWVISWRERTWWRNLRGGAKVRVLLAGRNVEGRGQVIEEEDHVAQSLFDYYRK
                     VPQVAKYVQIGLDATGAPVMADCERAAQKMVVVRIDLE"
     gene            complement(634..1116)
                     /locus_tag="VFQ23_25395"
     CDS             complement(634..1116)
                     /locus_tag="VFQ23_25395"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9910009.1"
                     /translation="MYVNARAIIEQETDEGIMIVVQVRNKPHEGSKRLELPGGRVEEF
                     ESLVSALKREVREETGLEITHIEGISTRVETGSQDANVECLVPFAVYQTLEGPVDSMG
                     VYFLCQATGQLLATGDDTEDIQWLPVQRVAQMMKTNPEKFDWVDRAGLRYYLQHKSLL
                     "
     gene            complement(1196..2020)
                     /locus_tag="VFQ23_25400"
     CDS             complement(1196..2020)
                     /locus_tag="VFQ23_25400"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9910010.1"
                     /translation="MSNLIKRNPLIAYFVIAFAFSWTVYFTLIAIKYGWMDVQIPMSI
                     HYLASFGPALAALIVTALTTGKEGLKELWARIVKWRIGWGYAVFAIFSPLAFFVLATI
                     IARFVKGEWLDVRLLGQANYLPYLGVWVLPLWLVTFGFGEEIGWRGFALPRLQKTMSV
                     QRATLTLALVWVLWHTPAFFYLDTIESLGGLIIIPGFVIGVLFGAVLLTWLYNGSGGS
                     ILAVAVWHALFDLTTASEAGQDIVPIVTTAGVILWALYVANVEKPWGFRLQEKHVL"
     gene            complement(2163..>2895)
                     /locus_tag="VFQ23_25405"
     CDS             complement(2163..>2895)
                     /locus_tag="VFQ23_25405"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET9910011.1"
                     /translation="TLFLVGLFLIGFAIFMGHENTQIEGALFGLLTGVVQMGVIHYAI
                     AKIFGPLLFDRMWCGWACWTVMILDLLPFKRPSGRLPRQYGWLRYLHFFLSLSIVLLL
                     VYVVGFREGATGTLAVTWFIIGNLLYYAVGIVLAYTLKDNRAFCKYVCPVSVPLKVTS
                     RFSIIKIGQGVGQCNDCDACEKMCPMDVRISDYILNNQRVLSTECSLCQTCITVCAQD
                     ALKLSFGFDVSKEDLLRERAPKVFR"
BASE COUNT          726 a          810 c          696 g          663 t
ORIGIN      
        1 catcgtctgg atcacaaatt tcgccgtcag ttcaatggag tattgctgtc gtctatttga
       61 taaatgcgag attgggattc gctttagcag tagctatgtc atgtggcgac gatcccacca
      121 ctattgcagt cttctgctcg attccgattg gcaacacata atggctgatc tttgggaatt
      181 ggcgatgttc aaaaactcat tccagatcga tccttaccac caccatcttc tgcgcggctc
      241 gctcgcaatc agccatgact ggcgcacccg ttgcgtccag accgatctgg acgtatttcg
      301 caacttgcgg gacttttcga taataatcga acagactctg agcgacatga tcttcctcct
      361 cgatgacctg tccgcgtcct tccacgtttc ggcctgccag cagaacccga accttcgcgc
      421 cgccccgtag atttcgccac cacgtccgct cgcgccaact gatgacccac aacgtatttc
      481 cgctgcacag ataattcgtt ggagtggaaa tggacttgcc actcttgcgt cccgtcatcg
      541 agatcaacat cacgcctttg ctaacgaatc catgcaacgg tgatttcaaa agccatatcg
      601 tcatcggatt gaaccacatc tcgtttctcc ttattagagc aaagatttgt gctgaagata
      661 gtatcgaagg cctgcccgat ctacccaatc gaatttctca ggattagttt tcatcatttg
      721 cgcaacccgc tggacaggca accattgaat atcttccgta tcatcgccgg tagcgagtaa
      781 ctgcccagtt gcctgacaaa gaaaataaac gcccatcgaa tcaacgggac cttcaagtgt
      841 ctggtacacg gcaaagggca ctaggcactc cacattcgca tcttgcgaac ctgtttccac
      901 tcttgtgctt attccttcga tatgggtaat ttcaaggccg gtttcttccc taacctctct
      961 tttgagcgcg ctgaccagcg attcaaattc ttccacgcgc ccaccgggca gttcaagccg
     1021 tttgctacct tcgtgcggct tgttgcgaac ctgtacaacg atcatgattc cctcatcggt
     1081 ttcctgttcg atgattgctc tcgcattgac gtacattttt catctcgctt tcattgtcgt
     1141 cgatatcctg attaccggat ttacattatc atcattcaat ctcgtggacc atcggtcaca
     1201 atacatgctt ttcttgaagg cggaatcccc atggcttttc gacattggcg acatacagcg
     1261 cccacaggat cacgcctgct gtggtgacga tgggaacgat atcctgcccc gcttccgatg
     1321 cggttgtcag atcgaataac gcgtgccaga ctgcgacggc aaggatgctg ccgcccgacc
     1381 cgttatacaa ccacgtcaat aacaccgcgc cgaaaagtac gccgatcaca aaaccaggaa
     1441 taatgatcaa gccgccgagg ctttcgattg tatccaggta aaagaatgcg ggcgtgtgcc
     1501 acaataccca gaccagggca agggtcaatg tcgcgcgttg gacgctcatt gttttttgca
     1561 ggcgcggcaa ggcaaatccg cgccagccaa tctcttcacc gaaaccaaat gttacaagcc
     1621 ataaaggcaa aacccaaaca ccgaggtaag gcaaataatt ggcttgaccg agaagccgca
     1681 catccagcca ttcgcccttg acgaagcgcg cgatgatcgt ggcaagaaca aagaacgcaa
     1741 ggggtgagaa aattgcaaat acggcatatc cccatcctat ccgccattta acgattctcg
     1801 cccacaattc tttcaaccct tctttgcccg tggtcaacgc cgtgacgatc agagcagcca
     1861 gggccgggcc gaatgaggcg agataatgga tggacatcgg gatctgcaca tccatccacc
     1921 catacttgat cgcgatcagt gtgaagtaaa ctgtccacga aaaagcgaag gcgatcacaa
     1981 agtatgcgat gaggggattt cgtttgatca ggttggacat ggttgatact cctatcatag
     2041 aaaagacctg acatgtcttc gcgaaagtct gacgattatg ctctgtgtcc gcacaatgta
     2101 tcgtgaaagc aaaggtgcgg acaagacatg tctggccccg ctgttaatcc gccgctacag
     2161 tctcagcgga aaaccttcgg ggcgcgctcg cggagcaggt cttccttgct cacgtcaaag
     2221 ccaaaggaca acttcaacgc atcctgcgcg cagacggtga tgcaggtttg gcataaacta
     2281 cattctgtgg aaagtacacg ctgattattg aggatgtaat cgctgatacg aacatccatc
     2341 gggcacatct tctcacaggc gtcgcagtca ttgcattgac ccacgccctg cccgatcttg
     2401 atgatcgaga agcgcgaggt gaccttgagt ggcacgctga cagggcagac gtatttgcag
     2461 aaagcacggt tgtccttgag tgtgtacgcc aacacaattc ccaccgcgta gtagagcaga
     2521 ttgccgatga tgaaccacgt cacggcgagc gtgcctgtgg caccttcacg gaaaccaacc
     2581 acgtagacga gcagcaggac aatgctcagg ctgaggaaaa agtgcaggta gcgcaaccag
     2641 ccatactgac gcgggaggcg tccgctgggt cgcttgaagg gaagcaaatc caggatcatc
     2701 acggtccagc acgcccatcc gcaccacatc cgatcgaaga gcagaggtcc aaaaatcttg
     2761 gcgatggcgt agtggatgac gcccatctgt accacgcccg tcagcaggcc aaagagcgcg
     2821 ccttcgatct gcgtgttttc gtgtcccatg aaaatcgcga agccgatgag gaagagaccc
     2881 accaggaaaa gcgtg
//
LOCUS       DASTJP010000876        13533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1138116, whole genome shotgun sequence.
ACCESSION   DASTJP010000876 DASTJP010000000
VERSION     DASTJP010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13533
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138116"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFQ23_25410"
     CDS             <1..844
                     /locus_tag="VFQ23_25410"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9910012.1"
                     /translation="YSGSEYRENLKSTGINPQLLFPRGILYPELRKMGVNPYVFGIRD
                     YTPSTYSNTVMAGAELRPFKTLSEALVNLGSLLEQETSKAYIYLYFDKIDALCHEYGP
                     TSLQAETEIETFLLIMEHYFERIFKGKKRILFLMTADHGVSEVDPNTTIYLNTEPRFT
                     GFSKFIETNRRGKLLVPAGSARDMFLYIKESMLDEAQDFLTGRLEGKADVVKTESLIV
                     DGYFGQDVSVRFRERAGNLVILPYRYESVWWYEKEKFEMRFYGHHGGLTSQEMETVLY
                     SYEL"
     gene            850..1401
                     /locus_tag="VFQ23_25415"
     CDS             850..1401
                     /locus_tag="VFQ23_25415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9910013.1"
                     /translation="MPNLSRFTNNDLPNHFAHQIRDFIRIHWFDIFQYDVHADAIGED
                     LKPVYFVVIDDPALFSHAAAVTRAVECNGQTYSCGGLSAVLTYPAFRNRGYGSQTVQA
                     ATEYLESASLDLALLWTSPDKQHFYGRFGWQYHPSIRILVGRPEAKEVYEAFTMIRFL
                     SDRATKNRSDFESHPIYVGEHSW"
     gene            complement(1445..2395)
                     /locus_tag="VFQ23_25420"
     CDS             complement(1445..2395)
                     /locus_tag="VFQ23_25420"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9910014.1"
                     /translation="MKLTTNSILYSLGVIGLLIIACVAITMLAVPLVQADQPGMDDAN
                     ATIQAIVTQTVYALTQNAPTSTSVPVTNTSVPPSNTPAPTSTPLPTSTTVSYCDWVTF
                     VKDVTIPDGTKFIAGETFTKTWRLQNRGTCTWTSDYMLVFSSGDPMGGTTAVRLPGTV
                     YPGQTVDVSVTLTAPAKRGRFVGYWMLRNPSGTLFGYGDNANRAFYVEIRTEDLPHGT
                     VSGNFQYPSEFNPPLILYFENAVTREIIQFSIPERNFTYSVLLPNGTYYAYAWAPGYN
                     LQGAFVNQNLTMKTFVVNGGQTTSGIHLTDWSPYPHSYGQ"
     gene            2549..3751
                     /locus_tag="VFQ23_25425"
     CDS             2549..3751
                     /locus_tag="VFQ23_25425"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HET9910015.1"
                     /translation="MKPIRWLIITTVLVFAACQSPNLPTVTIIDNERIIQLQTDKRVP
                     VDILNQAGITLLPEDRVLLNGSSVALDQSIADFPLILQIRRAATVVIASPEGQQTIQT
                     SAFTVGEALHETGIQLRAGEKVDPPLKSPLASSMEITIFPAAARELTFTADGMTFTAV
                     SSAQTVGEALAEAGIPLIGLDYSVPAENEELPPDGQVKAVRVSESVLLAQKPLPFESE
                     LQASADVPLDQTQILQPGETGLNVQRIRIRYDDGVETSRVSEDETLVRPSKTRLLGYG
                     TKVEIKTAVVDGVQIEYWRAVQMYATSYSPCRSGTDECHGGTSSGQSLRKGMVGLRQS
                     WYLSMQGQRLYIPGYGFSTVEDVCGGCVGRPWIDLGYSDNDYEPWSSWVTVYFLTPVP
                     ANIIYVLE"
     gene            3877..5034
                     /locus_tag="VFQ23_25430"
     CDS             3877..5034
                     /locus_tag="VFQ23_25430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9910016.1"
                     /translation="MTNSQKAILSFLSVLACFLVGLAAWLWREKWEQPLGPALNILTA
                     TPFQLPPTWTPDPAAIPPTPLASTPNSAITSPPTPTSTLPVVGMCGAPPLLNILVVGS
                     DARADTYTYGLADMIRLVRVDFVNPKVTIIEFPRDLWVEIPDIADNLNGQDHEKLNQA
                     YLYGNPGFGYTDDPARGPGLLARTLILNFGTQIDHYAAVNMRTFVKIVNAVDGIDVTL
                     PEAVDGRTAEDTNSRLLFRSGTLHLDGERALMLARIRIEGGFARADNQNRVLCALRDK
                     ITSPSVITKLPDLIASFQDAVQTDLSPAQLGQLACIGTQIQPGNIVFASFPEEHFTQS
                     RVFDPVFNKNVFAWDVDFEILREYIRQFNLGVWPSKSGTPEDNKEAIPFCS"
     gene            5031..5861
                     /gene="rsmA"
                     /locus_tag="VFQ23_25435"
     CDS             5031..5861
                     /gene="rsmA"
                     /locus_tag="VFQ23_25435"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET9910017.1"
                     /translation="MKEIPPLNAAALLKHYGLRAHKGLGQNFLQDPLALERIVEAADI
                     QPSDHVLEIGPGLGSLTRYLAAAAKEVVAVELDSNLLLPLEKILSPYDNAHVIQGDIL
                     KLSPSELINESNYIVVANIPYYITSAVIRHLLESDKKPRRIVLTVQREVAQRICAKPG
                     DMSLLALSVQVYGKPRIAARIPAGAFFPAPKVDSAVLTVDIYPSPLIQPELLETFFKL
                     IKAGFSQKRKTLRNALSSGLRISPSEAENLLRRADIDPQRRAETLSIEEWERLCKDFK
                     "
     gene            complement(5870..6631)
                     /locus_tag="VFQ23_25440"
     CDS             complement(5870..6631)
                     /locus_tag="VFQ23_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET9910018.1"
                     /translation="MRIVVVLPTYNEAENLRKLVSVLFSLPLDIIVLVVDDNSPDGTG
                     QLAEQLAGEHQNRVTVLHRAGKLGLRSAYIEGFRKAYEIGADAIVQMDADFSHDPQVL
                     TEMAGRLSSCDVVIGSRYVTGGSLDERWPLWRKALSAFGNNYARTILRFPLRDVTTGY
                     RMWKRDALMGMPLDRIRSNGYIFLVEMAYVAFLKGYDITEVPIHFADRRWGKSKMSLK
                     IQLEAAIRIWDVWWHYRDLHKQSHPHEIKGSDSHL"
     gene            complement(6631..7485)
                     /locus_tag="VFQ23_25445"
     CDS             complement(6631..7485)
                     /locus_tag="VFQ23_25445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9910019.1"
                     /translation="MADAKSKILIVEDDLDVAEMLNAYFRVQGYEVFTVNWGEDGVRA
                     AQTVLPDLVILDIRLPDIDGYEVARRIRTDRRTHEIPIIFLTEKRERIDRLQGFEVGA
                     DDYITKPFDVQELRLRVRNALKRMSQGSLTNPVSGLPEGPLVEERLSEVIHKSGWALL
                     HISINHLDTFREAYGFVASDDVMRAISLMIHNTMKETGSTEDFLGHLSPTDFAVVVSP
                     ANLEPFQERIRSRLEQSLDYFYPIKDREQASKRQDRLAIQISEVPSVYGRYTSVDQLK
                     KELLSKTT"
     gene            complement(7500..8135)
                     /locus_tag="VFQ23_25450"
     CDS             complement(7500..8135)
                     /locus_tag="VFQ23_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HET9910020.1"
                     /translation="MSPVQKTGLYYPNKFGLIIIKSLEEVMGKNGLNAILNLGGLNNY
                     IENYPPDNLDKGFDFAELSGIGVALEEMYGPRGGRGLALRAGRATFSDALKNFGALAG
                     AADLAFVVLPLQSKLRIGLPAFAKIFSQLSDQYSTVEEKDTEFIWTIHKCPVCWGRTN
                     ADKPVCYIATGLLQEALKWVSGGNEFRVNESKCVAVGDSICEFVIQKEPIS"
     gene            complement(8148..8519)
                     /locus_tag="VFQ23_25455"
     CDS             complement(8148..8519)
                     /locus_tag="VFQ23_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9910021.1"
                     /translation="MSKSRTQQMVDRLRELQASSPDIEASAVVSVDGLSIASALPQGV
                     EEDRVSAMSAAMLSLGERIATELGRGSLEQVYIKGEKGYVVLMSVGEEAVLTALAREQ
                     AKLGLIFLDIKRAAEDLAKLI"
     gene            8791..9933
                     /locus_tag="VFQ23_25460"
     CDS             8791..9933
                     /locus_tag="VFQ23_25460"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9910022.1"
                     /translation="MKKIFLFAQILVFISIFATPSPINAQESEPESGAVVCAPGVYLE
                     TPSDCLPLGPSAFLTDMAKLGMTFPPRLLPSRIPDPALTQLPYFYFMLEEDIVPILSG
                     PAGSETGQAFQPGFVYVSYIDRVESNGVYYMLQNGGWIPGKGSRIGDYSRFQGLEFKS
                     TPRNSFAWPLPFYTGSIPVYKAPGYNAPLTDRILYPYVDIVQVYATQNADGVDWNLIG
                     PNQWVEARILATVIPNTTPPEGVTTGRWIDVDLAEQTLAVYDNYQLVFATVIASGLEP
                     FWTRPGLFQIYQKKETETMRNNDPTDFYYLDNVPWTMYFDKARALHGAYWRTRFGYPQ
                     SHGCVNLSVGDAHWLYNWALEGDFVYVHDPLGLTPTDPALYHDWAY"
     gene            9981..10054
                     /locus_tag="VFQ23_25465"
     tRNA            9981..10054
                     /locus_tag="VFQ23_25465"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10015..10017,aa:Asp,seq:gtc)
     gene            10242..13097
                     /locus_tag="VFQ23_25470"
     CDS             10242..13097
                     /locus_tag="VFQ23_25470"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9910023.1"
                     /translation="MLEVRLLGKFELKSDGKFVAIPSRPAQSLLAFLIMSAGTSHRRE
                     KLAGMLWPDSLEEAARSNLRSALWKIRKALPSSVVVEYLFTDDLTVAFNDSAEYWLDA
                     AELAKATEKASANELMTTLSLYYGELLPGFYDEWVVLEREHLSSVFEHHMARLMSSLE
                     SEHRWLDILDWGERWIKLGQKPEPAYRALMSAHAAKGDISKVAASYERCVKSLKEFGI
                     EPSEQTRNLYESLKSGKERVETHPTAAVVKKRKSPSKTNLPVPITSFIGREKEVEELV
                     TLVGKNRLVTLAGSGGVGKTRLAIQSSHKLLTKFKDGIWWIDLVGLSDPALVPQAVAK
                     VLDVREIPNQPLADTLVGELQTKQILVVLDNCEHLILACARIADRLLSGTKHLKILAT
                     SREALEILGETVWPVPSLSLPDPQDSVTIKSLSKSESVRLFAERANAMQRKFTLTDQN
                     VNAVAQVCRRLSGMPLAIELAAARIKLMSVDEIAKRLDDRFSLLTSGNRSALPRQQTL
                     RATIDWSYDLLTDPDKMLFRRLAVFAGGFTLEAAESIGSDNSMPHSDVLDVLGRLVDK
                     SLVVVDAAADSGQTRYRFLETIREYALEKLRSAGEETAIRDQHLEYFRRLAEETEPHL
                     YASEQAVWFARTDAEIDNLRAALDWSVVEDPGVKNDARIRNGFQLVGVLTWYWRRSYL
                     HDFIERLEHMLSREIGVAPSLERARALYTLGFFYGTQEAAKARPYLEQALDIAQKHND
                     PLTLSWALTHLAVVTCLLQEYDSSLSLLEESLALTRELGISGKALTGMTLEWMGEDYL
                     GLGDEVRAQELFEESIALLRETNNRDLLSFADRRLGLLMLKQGNYERAEILLKESLQM
                     NHYIGHAPGTAGSLTALAKLALVQGNLTRSAQLYGMVDGRLEVIKAPMLVMDVDEYDN
                     GVTTLRSQLDEKTFENFWMKGRATTLEGAIRFALEEA"
     gene            13282..13425
                     /locus_tag="VFQ23_25475"
     CDS             13282..13425
                     /locus_tag="VFQ23_25475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910024.1"
                     /translation="MLITILLVTLFVLVQAVCFVIAWKQAPDEQDLSDEEREAALMSF
                     YHP"
BASE COUNT         3384 a         3350 c         3386 g         3413 t
ORIGIN      
        1 gtactccggt tctgaatatc gggaaaattt aaagtctaca ggcatcaatc cccaactttt
       61 gttcccgcgc gggatcctgt atcctgaact gagaaagatg ggagtcaatc cttatgtgtt
      121 tggcatccgc gattacacgc catctaccta ttccaatact gtgatggcgg gcgccgagtt
      181 acgtccgttt aagacgctgt cagaggcttt ggtcaatttg ggttccttgt tggagcaaga
      241 gacaagcaag gcatacatct atctttattt cgacaaaatc gatgcgctgt gccatgagta
      301 tggtcctacc tccttgcaag cggaaacaga gatcgagacg tttctgttga tcatggaaca
      361 ttacttcgaa cgcattttca aagggaagaa aagaattttg ttcctgatga cagccgacca
      421 cggcgtatct gaagtggacc cgaacaccac aatttatttg aacactgagc cgcgctttac
      481 aggatttagc aaatttatcg aaacgaaccg gcgagggaaa ttacttgtac cagcgggttc
      541 tgcgcgcgat atgtttcttt acatcaaaga gtctatgctt gacgaggccc aggactttct
      601 taccgggcgg cttgagggca aagcggatgt agtgaaaaca gagtcgctga ttgttgatgg
      661 atacttcggc caggatgtct cagttcgttt tcgagagcgg gctgggaatt tggttatact
      721 cccctatcgc tatgaatctg tgtggtggta tgaaaaggaa aaatttgaga tgaggtttta
      781 cggtcaccac ggcgggttga cgtcacagga aatggaaacg gtgttgtatt cttatgagtt
      841 atgaatgtta tgcctaatct aagccgcttc accaacaacg atctaccgaa tcattttgcc
      901 catcagatac gggattttat ccgtatccat tggtttgaca tctttcaata cgatgttcat
      961 gctgatgcta tcggtgagga cttgaagcca gtttactttg tcgtgataga cgaccctgcc
     1021 ttattcagtc acgcagcagc ggtgacgcgc gctgttgaat gtaacggcca gacttacagt
     1081 tgcggtgggt tgagcgcagt cctcacttac ccagccttca gaaatcgcgg gtacggctcg
     1141 cagacggtgc aagctgcgac cgagtattta gaatctgcct ctctagacct ggccctgctt
     1201 tggacctcgc ctgataaaca acacttttat gggcgttttg gctggcaata tcatccatcc
     1261 attcgaatcc tcgtcggacg acccgaagcc aaagaggtgt atgaggcttt caccatgatt
     1321 cgctttctat cggaccgagc cacaaaaaac cggtcagatt tcgaatctca tcctatctat
     1381 gtcggtgaac attcctggta actgaaaaga agaaggcgac cgatgggtcg ccttcttctt
     1441 tatattattg cccataactg tgagggtaag ggctccagtc cgtcaggtgg attccactgg
     1501 tggtctgccc accgttgacg acaaacgtct tcattgtgag attttggttt acaaaggctc
     1561 cctgaagatt gtaaccaggc gcccaggcat aggcatagta ggttccattt ggaagcaaaa
     1621 cgctgtatgt aaaattccgc tcgggaatag aaaactgaat gatctcccgt gtcacagcat
     1681 tttcaaagta caggatcaac ggcggattga actcacttgg gtactgaaag ttaccactga
     1741 ccgttccatg aggcaaatct tccgttctaa tctcaacgta gaaggctcgg ttcgcattat
     1801 caccatatcc gaacaaggtt ccagatggat tccgcaacat ccaatagcca acaaagcggc
     1861 cacgtttggc gggtgcagtc agtgtgacag agacgtccac agtctgtccg ggatatacag
     1921 ttccgggcaa acggacagcc gttgtgccac ccataggatc accacttgaa aataccagca
     1981 tatagtcaga ggtccatgtg caggtgccgc gattctgcag gcgccaggtc tttgtgaacg
     2041 tttctccggc gatgaatttg gttccatccg ggatggtaac gtcctttacg aaagtgaccc
     2101 aatcacaata ggaaacggtc gttgaggtgg gcagaggcgt gcttgtggga gccggcgtgt
     2161 tagaaggagg aacagaggta ttggtcaccg gcaccgaggt tgaggtaggc gcgttctgtg
     2221 tcagtgcgta gactgtctgc gtaacgattg cctgaatcgt cgcattggca tcatccatcc
     2281 cgggctggtc agcctgtacg agtggcacag ccagcatggt aatcgcaaca caggcgatga
     2341 tcaacagtcc gatcacacct aatgaatata atattgagtt cgttgtaagt ttcatgtcgc
     2401 acctccaata atgtgcggag atgtgacgag ttcccatttt ggaaagttcc ctgtagcaca
     2461 aaaattagta ctgaaaggtt aatcccggtc tcataaggcg ttacatcaac aggaatagtc
     2521 tcggcgccaa ctgctttata attaactcat gaagcccatt cgttggctga tcatcacgac
     2581 agtcttagta ttcgctgcct gccagtcgcc taatctcccc acggtcacaa tcattgataa
     2641 cgaaagaatc attcagcttc aaacggacaa gcgcgttcct gtggacatcc ttaaccaggc
     2701 aggtataact ctccttccgg aagaccgcgt cctactcaat ggttccagcg tcgcgctgga
     2761 ccagagcatt gctgatttcc cattaatttt acaaatccgt cgtgcagcga cagtggttat
     2821 cgcttcgcct gagggtcaac aaacgataca aacctctgcg tttaccgtcg gtgaggcatt
     2881 acatgaaacc ggtattcaat tgcgagcagg agaaaaagtt gacccgcccc tgaagtctcc
     2941 tctagcttct tccatggaaa tcacaatttt cccagccgcc gcccgtgaac tcacctttac
     3001 cgccgacgga atgacattta ctgcagtgtc gtccgcacaa accgtcgggg aagcgctggc
     3061 ggaagctggg attccgctga tcggtctgga ctacagcgta cctgcggaga atgaggagct
     3121 tccacctgat gggcaggtca aagctgtccg tgtgagcgag tccgttttgc tcgcgcaaaa
     3181 gcctcttccc tttgagagcg aacttcaggc ttcggcggac gtgcctcttg atcaaacaca
     3241 gattttgcag cccggcgaaa ctggactcaa cgtacagcgc attcggattc gatatgacga
     3301 tggggtcgaa acatcccgcg tctctgaaga cgagaccctg gttcgccctt caaaaacgcg
     3361 cttgctgggt tacggaacaa aggttgagat taagacggcc gtagtggatg gtgtgcaaat
     3421 cgaatattgg cgcgccgttc aaatgtatgc gacctcttat tcgccctgcc ggtcaggaac
     3481 ggatgagtgc cacggtggaa catctagcgg acaatctctg cgaaaaggga tggtaggatt
     3541 gcggcaaagc tggtatttga gtatgcaggg acagcggctt tacatcccgg gatatggatt
     3601 ttcaacggtt gaagatgtgt gtggtggctg cgtgggcaga ccatggatcg atcttggcta
     3661 tagtgataat gattatgagc cctggagttc gtgggtaacc gtatatttcc ttacccccgt
     3721 gccagcgaac ataatttatg ttttggagta atattctcgc gctgagcagc gctctgagtg
     3781 taagtcgaag agtgcaggcg aagctcgaga tgaaataaag acgcgcttcg tctctgccgc
     3841 aaacaagtgc ggctccgctc agcgtgaagc gaaataatga caaattctca aaaagcaatt
     3901 ctctcatttc tttctgttct tgcctgtttt ctcgttggtc tcgctgcttg gctatggagg
     3961 gagaaatggg agcaaccact aggacccgcc ctaaatatct taacagccac tccttttcaa
     4021 ctaccgccta catggacgcc tgatcccgcg gcaattcctc ccactcccct tgcatcgaca
     4081 cctaactccg caattacgtc accgccaaca ccgacaagta cccttccggt tgttggcatg
     4141 tgtggcgcgc cacctctgtt gaacattctt gttgtgggca gtgatgcacg tgcggacacc
     4201 tatacgtatg gtctagccga tatgattcgc ctggtgcgcg tggattttgt gaaccccaaa
     4261 gtgaccatca ttgagtttcc gcgcgaccta tgggtggaaa tcccggatat cgcagataac
     4321 ctcaatggac aggaccacga aaaacttaat caggcttatc tgtatggcaa tcccggcttt
     4381 ggttataccg atgacccagc ccgtggaccc ggactactag cgcggactct catcctaaac
     4441 ttcgggactc aaatcgatca ttatgccgcg gtcaatatgc ggacgtttgt gaaaatcgtg
     4501 aatgcggtgg atggaatcga tgtgaccctg cccgaagccg tggatggtcg caccgcagag
     4561 gacacgaata gccgcctgct gtttcgttct ggtacccttc acctggatgg agaacgcgct
     4621 ctgatgctcg cgcgtattcg cattgaaggc ggttttgcac gcgcagataa tcaaaatcgt
     4681 gtgctatgtg ccttgcgaga taaaataacc agcccatctg tgattacaaa gctgcctgat
     4741 ctgattgctt catttcagga tgccgttcaa acggatttaa gtccagcaca gcttggtcag
     4801 cttgcctgta ttggcacaca gatccagccg ggaaatattg tctttgcaag ttttccggaa
     4861 gaacatttca cgcaatcacg agtcttcgac cccgttttca ataaaaacgt ctttgcctgg
     4921 gatgtagact ttgagatcct tcgtgaatac attcgccaat tcaacctcgg cgtttggcct
     4981 tcgaaatcgg gcacgccaga agataataaa gaagccatac cattctgttc atgaaagaaa
     5041 ttcctccact caacgccgcg gccttgctga aacactatgg cttacgcgcc cataagggtc
     5101 tcgggcagaa ctttttacag gaccctctcg ctcttgaaag aattgtagaa gctgccgata
     5161 ttcaaccgtc agatcatgtt ctggaaatcg gcccagggct tggtagctta acgcgttatt
     5221 tggctgccgc cgccaaagag gtcgttgcgg tcgaactgga ctcaaacctg ctcttgccat
     5281 tggaaaagat cctttcgcct tacgacaatg cccatgtaat ccagggcgat attctgaaac
     5341 tttctcccag tgaattaatt aatgagtcaa attacattgt tgtagcaaac attccttatt
     5401 acatcacctc tgccgtaatt cggcatttat tggaaagcga taaaaagcct cggcgcattg
     5461 tcctgacggt tcaacgagag gttgcccaac ggatttgtgc gaagccaggt gacatgagtc
     5521 tgctggcgct gagcgtgcag gtgtatggaa aaccgcgcat tgcagcgcgt attcctgccg
     5581 gcgctttctt ccccgcgccg aaggttgatt cagcggtgtt aactgttgat atttaccctt
     5641 cgcccctcat acaacctgaa cttcttgaaa cattcttcaa gcttatcaaa gccgggttca
     5701 gccagaaacg aaaaacattg cgcaacgcgc tctcttccgg tttgcgcatc tctccatcag
     5761 aggcagaaaa tctgctacgt cgcgcggaca tcgatccaca gcgccgggcg gagacgctaa
     5821 gtattgaaga atgggaaaga ttatgcaaag atttcaagtg acagtcacct taaaggtgac
     5881 tgtcacttcc tttgatctca tgaggatgac tctgcttatg caaatcccga taatgccacc
     5941 agacatccca tattcgtatc gcggcttcca attgaatctt gagtgacatt tttgattttc
     6001 cccagcgacg gtccgcaaag tgaatcggga cttccgtaat atcatatccc ttcagaaaag
     6061 ctacatacgc catttccacg aggaagatat acccattcga gcggatccga tccagtggca
     6121 ttcccattaa ggcatcgcgc ttccacatgc gataacccgt cgtcacgtca cgcagcggga
     6181 accggaggat cgtacgggcg tagttatttc cgaaggcaga tagcgctttt cgccacaggg
     6241 gccaacgctc atcgagagat ccgccggtca cataacggga gccaatcaca acgtcgcagg
     6301 aggagagccg gccggccatc tctgtcaaaa cctggggatc atgcgaaaag tccgcatcca
     6361 tttgaacgat cgcatcagca ccgatctcat aggctttgcg aaacccttca atatatgcgg
     6421 atcgcaatcc cagctttccg gctcgatgca acactgtaac tctgttttga tgctcgccag
     6481 ctaattgctc agcgagttgt ccggtgccat ccgggctatt atcgtccacc accagaacta
     6541 tgatgtcgag cgggagcgaa aataaaacgg agaccagctt tctcaggttc tccgcttcgt
     6601 tataagttgg taatacaacg acgatgcgca ttaagtagtc ttgcttaata gctccttctt
     6661 caactggtca acacttgtgt accgcccata gacagaagga acttcagaga tttgtatagc
     6721 aaggcgatcc tgtcgtttgg atgcctgttc acgatctttg atgggataaa agtaatccaa
     6781 cgattgctcg agccgggagc gaatccgttc ctggaacggc tcaagatttg cgggggaaac
     6841 cacaactgca aaatccgtag ggctgagatg cccaagaaaa tcttcggttg aacccgtttc
     6901 tttcatcgtg ttatggatca tgagactgat ggcgcgcatc acatcatcgg atgcaacaaa
     6961 gccgtatgcc tcacggaatg tgtcaagatg atttatcgaa atgtgaagca aggcccagcc
     7021 gcttttgtga atcacctccg aaaggcgttc ctccacgaga gggccttccg gcaacccact
     7081 gaccgggttt gtcagcgatc cctggctcat ccgtttcaaa gcatttcgca ctcgtaaacg
     7141 gagttcctgc acgtcaaaag gtttggtgat gtaatcatcg gcgccgacct caaagccttg
     7201 aagtcgatca atgcgttcgc gtttttcagt caagaatatg atggggattt cgtgtgtacg
     7261 ccggtcggtt cgaatccgcc gggcaacttc gtacccgtcg atatctggca ggcgaatatc
     7321 aagaattaca agatctggaa gcacggtctg agccgcgcgc acgccatctt ctccccagtt
     7381 aacagtaaag acctcatagc cttgaacgcg aaaataggca ttcaacatct cggctacatc
     7441 gagatcatct tcgacgatta gaatcttgga tttggcgtca gccacgtcaa cccaatccgc
     7501 taagaaatag gttctttctg gatgacgaat tcacaaatgg agtcgcccac ggcaacacat
     7561 ttggattcgt tgacgcggaa ctcattcccg ccagagaccc atttgagggc ttcctgtaac
     7621 aaacctgtgg cgatatagca cacgggtttg tctgcgtttg tgcgtcccca acatacaggg
     7681 cacttatgga tggtccagat aaattccgta tctttttctt cgaccgtact atactggtcg
     7741 ctcaactgag agaaaatctt tgcaaaggct ggcagtccaa tgcgaagttt ggattgcaga
     7801 ggcaggacta cgaaggccaa atcggccgca ccggccaggg caccaaagtt cttcaacgcg
     7861 tctgagaaag tggcgcgacc cgcacgcaga gccaaacctc ggccccctcg aggaccgtac
     7921 atttcctcca gggctacacc gattccagag agttctgcga aatcaaatcc tttatccagg
     7981 ttgtctggtg gataattttc aatatagtta tttaaaccgc ccaggttaag aatggcattc
     8041 aggccatttt tgcccatcac ttcttcaaga gatttaataa tgatgagtcc aaatttgttg
     8101 ggataatata aaccagtttt ttgaacaggg ctcatgcttg actccactta gatcagtttc
     8161 gccagatctt cggcggcacg tttgatatcc aggaagatca agcctaattt tgcttgctca
     8221 cgcgccagcg cggtaagaac ggcttcttcc cctaccgaca taaggacaac atatcctttt
     8281 tcgcctttga tataaacctg ctcgagcgaa ccacgcccca attcggttgc aatacgttca
     8341 cctaaagata acattgcagc agacatggct gacacgcggt cttcttcaac cccctgaggt
     8401 aaggcagatg caatgcttaa tccatccacg ctcactactg ctgaggcctc tatatccggt
     8461 gatgaggctt gtaattcgcg cagacgatcc accatttgtt gcgtacgtga ctttgacaaa
     8521 acgaccctcc tgcacaaaaa tgttcccaag ccgaataaaa ggctggtttg attgcaatag
     8581 aattgcagat aatttagcat agtggacaga aagtgtcaag ccagcctcca aataagaggc
     8641 ttctcatttg caaaaatgta aagccagcca tgttacactg tgccgcgtat acatgctgag
     8701 cttagagctt gagtttctac accagaaatt cctaaaaacc tgaaaggtcg tccgtcaagc
     8761 ggtaaaatta acccgtacct gtattagccc atgaaaaaga tatttttatt cgcacagata
     8821 ctcgttttta tctcgatctt tgcgactccc tcaccaatca acgcccagga atctgagcca
     8881 gaaagtggag ctgtggtctg cgcaccgggt gtatatttgg aaacgccgag tgactgtctg
     8941 ccccttggtc cctcagcttt tctgacagat atggcaaaac tgggaatgac cttcccaccc
     9001 cgcctgttgc cttcacgtat tccggaccct gcacttactc agctgcccta tttttacttc
     9061 atgctggaag aagatattgt ccccatctta agcggacccg ctggcagtga gactgggcag
     9121 gcatttcaac ccgggtttgt ctatgtgtct tatattgatc gtgtcgaatc caacggagtc
     9181 tattacatgc tgcaaaacgg cggatggatt ccgggaaaag gttcacggat tggagattat
     9241 tcccgctttc aagggcttga gttcaagtcc accccgcgta actcttttgc gtggcccctg
     9301 ccgttttaca cggggagcat tccggtttat aaagcaccag gctacaatgc gccgctcacc
     9361 gatagaatcc tttacccata tgttgacatt gttcaagttt atgccacgca aaacgcggat
     9421 ggcgtggact ggaatctgat tggacccaat cagtgggtag aggcgcgcat cctcgcaaca
     9481 gttataccaa acacgacacc cccggaagga gtaacaaccg gtcgctggat cgatgttgat
     9541 cttgcagaac aaacgctggc agtctacgac aattatcagt tggtctttgc cacggtcatc
     9601 gctagtggac tcgaaccgtt ctggactcgt ccggggctgt ttcaaatcta tcagaaaaaa
     9661 gaaacagaga ccatgcgcaa taacgacccc actgatttct attatctgga caacgtcccc
     9721 tggacaatgt acttcgataa ggcacgcgcg ctgcatggcg cgtattggcg tactcgattt
     9781 ggttatcctc aatcacatgg atgtgtgaac ctatccgtcg gcgatgctca ttggttatac
     9841 aactgggctc tcgagggcga ttttgtatac gtccatgatc cgttaggtct aacaccgacc
     9901 gatccggcac tctatcatga ctgggcatat tgacacttgt taatgcgcat gataaactct
     9961 gcccgcttgt taagctgaat ggtcccgtgg tgtagcggcc taacatgcgg ccctgtcaag
    10021 gccgagatcg cgggttcgaa tcccgtcggg accgcctaga gagacaactt gatgattttt
    10081 catcaagttg tttttgtttt ttatactaat cagagggatc gtcatttacc tggggcggac
    10141 cctgaattca tcagcgatga gcagattttc cgaactaatc acgagatcag aagccgttcc
    10201 aatatagtgt tcttcaatct gctatacttt gcaacatact gatgctcgaa gtccgattgc
    10261 ttggaaaatt tgagttaaag agcgacggca aatttgtcgc gatcccttcg cgccctgcgc
    10321 aatccttgtt ggctttctta attatgagtg cagggacatc tcatcgccgc gaaaaattgg
    10381 ccgggatgtt atggccagat tctttagaag aggcggcaag aagcaatctg cgcagcgccc
    10441 tttggaaaat tcgtaaagca cttccttcct cagtcgtagt tgaatacctt tttacggatg
    10501 atctgaccgt tgctttcaat gattcggcgg aatattggct cgacgcggcg gagttagcaa
    10561 aagcgactga gaaagcatcg gcgaatgaat tgatgaccac cctctcactg tattacgggg
    10621 agctgttacc gggcttttat gatgaatggg tggtgttgga gcgcgaacat ctgtcttctg
    10681 ttttcgaaca tcacatggca cgactaatgt cgtccctgga aagtgagcat cgctggctgg
    10741 acatcctgga ttggggcgag cgttggatca agctgggaca aaagcctgaa ccagcgtatc
    10801 gcgccttgat gtctgctcat gctgcaaaag gggatatttc caaggtcgca gcgagctatg
    10861 agcgctgtgt gaagtccttg aaggaatttg gcattgaacc ctcggagcag acgcgcaatc
    10921 tgtatgaaag tctcaagtca gggaaagaac gtgtggaaac ccatccaact gccgcggtcg
    10981 taaagaaaag aaaaagccca tcaaaaacga atctgcccgt ccccatcacc agctttatcg
    11041 gccgtgagaa agaagtcgaa gagttggtca cgctggtggg gaagaatcga ctcgttacat
    11101 tggcgggttc gggtggggtt ggaaagactc gtttggcgat ccaatcttcg cacaagctgt
    11161 tgaccaaatt caaagatggt atatggtgga tcgatttagt tggcttgagc gatcctgcgc
    11221 tggttcctca agcggttgcc aaagtgctgg acgttcgtga gatacctaat caaccattgg
    11281 ccgacacgct ggtcggagaa cttcaaacga agcagatttt agtggtattg gataactgtg
    11341 aacatttaat ccttgcctgt gcccgaattg cggaccgcct gctaagtggc acaaagcact
    11401 taaagattct tgcaaccagt cgcgaagcgc tcgagatttt gggagaaaca gtatggcccg
    11461 ttccgtctct ttcattgcct gatcctcagg attctgtaac cataaaatcc ttgagcaaat
    11521 ccgagagtgt tcgattgttt gccgaacgag caaatgcgat gcagcgtaag ttcacgctga
    11581 ccgatcagaa tgtcaatgca gtagcccagg tttgtcgccg cttgagtggg atgccgcttg
    11641 ccattgaact ggcggcagcg cgcatcaaac tgatgagtgt cgatgaaatt gctaaacgtc
    11701 tcgatgaccg tttttcccta ttaacttctg gcaatcgctc ggcgctgccc cggcaacaga
    11761 ctttacgggc tacgattgat tggagttatg atctgctgac tgatcctgac aagatgttgt
    11821 ttcgccggct cgcggtattc gcgggaggtt ttacgctgga ggctgccgag tcgattggca
    11881 gcgacaattc catgccacac agtgatgttc tcgatgtact tggacggctg gtcgataaat
    11941 cccttgtggt agtagatgct gctgccgata gcggacagac ccgctatcgt tttcttgaaa
    12001 ctattcgtga atatgcgcta gaaaaactga gaagtgccgg cgaggaaaca gccattcgtg
    12061 atcaacattt ggaatacttt agaagactgg ccgaggaaac tgagccgcac ttatacgcat
    12121 ccgaacaggc ggtatggttc gccaggacag atgcagaaat cgacaacctg cgcgccgcgc
    12181 ttgattggtc agtcgtggaa gatcctggag taaaaaatga tgcgcggatt cgcaatggct
    12241 ttcagttagt gggggtatta acatggtact ggcggcgaag ttatttgcat gatttcatag
    12301 agcgattaga acacatgctt tctcgcgaga tcggagtggc gccatccctt gagcgggcca
    12361 gagcgctata tacattggga ttcttttacg ggactcaaga agccgcaaaa gcacgacctt
    12421 atctggagca ggcactggat atcgcgcaaa aacataatga tccccttacc ctgagctggg
    12481 cgttgactca cctggctgtc gtaacctgcc tgctgcaaga gtatgactcc agtctttccc
    12541 tcctggagga gagccttgca cttacaagag agttggggat ttcaggcaaa gcccttacgg
    12601 gtatgacgct cgaatggatg ggagaggatt atttagggct tggggacgaa gtccgcgcac
    12661 aagagctctt tgaggaaagc atcgcattgt tgcgagagac gaacaaccgc gacctattgt
    12721 cttttgctga tcgcaggctg ggtctgttga tgttgaagca gggcaactat gagcgggcgg
    12781 agatcttact caaggaaagt ctccagatga accattatat aggccatgcg cccggtacag
    12841 cgggttctct gactgcactg gcaaaactgg cgctggtgca gggaaacttg acgcgttctg
    12901 ctcaattgta tggaatggtg gatggcaggc tggaggtgat aaaggcgccc atgctggtta
    12961 tggatgtaga cgagtatgat aatggtgtca ctacactgcg ctcccagttg gacgagaaaa
    13021 cgtttgaaaa tttctggatg aagggcaggg ccacgacgtt ggagggagcc atccgattcg
    13081 cgttggaaga ggcgtgacaa tttatgtttg ccgaccttgt cccgccgctg aaatcagcgg
    13141 cgggattttt atttcccacg gtctgttcaa tagccgaaaa aatacgctca atctacgcgc
    13201 caaaatacgc taagaaacgc ggtgcacgta cactgaggac atagattgac caaatcacca
    13261 tcatccagtc aggagaccac catgttaatc actatccttc ttgtcaccct ttttgtgctt
    13321 gtccaggccg tatgctttgt catagcctgg aagcaggctc ccgatgaaca agacctaagt
    13381 gacgaggagc gcgaagctgc cttgatgagt ttttatcatc cgtaagctcg aaagggaaaa
    13441 tacagtgaat atcacccatc aagacgtgtt gagatcgccc cagctattcg aagatggcac
    13501 ctttgagtat ctggggttta ttacctctct ccc
//
LOCUS       DASTJP010000877         3732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1247498, whole genome shotgun sequence.
ACCESSION   DASTJP010000877 DASTJP010000000
VERSION     DASTJP010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247498"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFQ23_25480"
     CDS             complement(<1..925)
                     /locus_tag="VFQ23_25480"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET9910025.1"
                     /translation="MEISRSIALFEEAQTLLPGGVDSPVRAFRAVGGQPLFIERGEGP
                     YLYDVDGNRYIDYVLSWGPLITGHAHPHIVKAIQATVLKGTSYGAPSPLENELAKAVI
                     ALMPNIELIRFVNSGTEATMSALRLARAYTQRDKFIKFEGCYHGHADLMLVQAGSGVV
                     TLGLPDSPGVPANTVKDTLVADFNNLESVEALFRKYPEQIAAVIVEPVAGNMGVVPPQ
                     PGFLEGLRAITEREGSVLIFDEVMTGFRVHKGGAQTLYNIKPDLTTLGKVIGGGLPVG
                     AYGGKKEIMQLVAPAGPVYQAGTLAGNPLAMS"
     gene            complement(935..1975)
                     /gene="hemB"
                     /locus_tag="VFQ23_25485"
     CDS             complement(935..1975)
                     /gene="hemB"
                     /locus_tag="VFQ23_25485"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET9910026.1"
                     /translation="MTLLELKTRTSLSSVALRGRPRRLRTTPALRAMLRETELNARDF
                     IYPLFVRHGVGRSAIQSMPGIHQLSVEESVREAEAAARSGVSAVILFGIPNRKDPIGL
                     ENFAYDGIVQQAIRAIKKEIPEMVVITDVCLCEYTDHGHCGILNTGEHFHAGLPEGYV
                     LNDETLDVLAKVAVSHAESGADILAPSGMMDGMVFSIREGLDLAGYEHLPILSYAVKY
                     ASSFYGPFREAAEGAPKFGDRKSHQMDPANVHEALREAALDVQEGADMLMVKPALAYL
                     DVIRVVKDMYPEVPLAAYNVSGEYAMVKAAAANGWIDEAKVTLEALTGIKRAGADLII
                     TYHALEAAKWLT"
     gene            complement(2042..2815)
                     /locus_tag="VFQ23_25490"
     CDS             complement(2042..2815)
                     /locus_tag="VFQ23_25490"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET9910027.1"
                     /translation="MNLTNKRVLITRPRAQAEEFANTLKAVGAKPIFFPVIKIVPPDN
                     FSVVDFAIRSLKHYDWLILTSIHGVDAFFKRLDFLGIKKIPVGLNVAAVGAKTAQRIS
                     EYGIQVDYLPEEYLSEAISAGLGNKVYGKRFLFPQSNLARTVLANEIRNARGIVTEIV
                     VYRTVLNTPDSIALDALRAGVEIVTLTSPSTVQNFIAVVQKNGLDPFRLPGDPLFACI
                     GPITERAAREQGLSNVVMAKEYMMEGLIEEISKHTQVHT"
     gene            complement(2835..>3732)
                     /gene="hemA"
                     /locus_tag="VFQ23_25495"
     CDS             complement(2835..>3732)
                     /gene="hemA"
                     /locus_tag="VFQ23_25495"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET9910028.1"
                     /translation="LGQVTRALKVARGEHATGPILNRLFQAAIHAGKRARAETTISRN
                     PASVSSLAASVAEKALGQIKSASVVILGAGEAGELMVEALRKRGVDNIKVINRTLARA
                     QELARLWNAEAVPFEFLQQALCETDILISSTSALQFVITKQMVRQAMETRTHRPLVLI
                     DLAVPRDIDPSVAGIPHVKLFDIDGLNEQLESSLASRMNEVPRVQQILDEEASLFRQF
                     LNSLAVLPMIAEMHEQAETIRAAELQKTFRHMPNLTDAERKRIEALTQSLVKKILDQP
                     TRHLRNESAPSDASRYPLLINN"
BASE COUNT          917 a         1042 c          896 g          877 t
ORIGIN      
        1 cgctcatcgc gagtggattt cccgccagcg tccctgcctg atacacgggt cccgcgggcg
       61 ccaccagttg catgatctcc tttttcccac catacgcacc caccggaagc ccgccaccta
      121 tgaccttacc cagcgtggtc aaatcgggct tgatgttgta cagagtctgt gcaccacctt
      181 tatgcacacg aaaccctgtc atcacttcat cgaagatcaa aacgctgcct tcgcgttcgg
      241 tgatcgcacg caagccttcc aaaaatccgg gttgaggtgg gacaacaccc atgttccccg
      301 cgactggctc aacaatgacg gctgcaatct gctcaggata tttcctgaac agggcctcca
      361 ccgattcaag gttattgaaa tctgccacca atgtatcttt gacggtgttc gctggcacgc
      421 ccggtgaatc cggtaagcct aaagtcacaa cacccgagcc tgcctgtact aacatcaggt
      481 ctgcatgacc gtgataacag ccttcgaact ttatgaactt gtcgcgttgg gtgtaggcgc
      541 gcgccaaccg taatgcagac atggtggctt ccgtcccgga attaacgaag cggatcagct
      601 cgatgtttgg catgagcgct attacggctt ttgcgagctc attttccagt ggacttggcg
      661 cgccataaga tgttcccttg agcacagttg cttgaattgc tttcacaata tgagggtgcg
      721 cgtgaccagt gatgagaggt ccccaggaaa gtacataatc aatgtaacga ttgccatcca
      781 catcatatag atacggcccc tcaccgcgtt cgatgaagag aggctgccca cccacggcgc
      841 gaaaggcgcg gaccggtgaa tccacgccgc ctggcaggag agtctgggct tcctcaaaca
      901 aagcgatgga tctagagatc tccatgaaat cccatcacgt caaccacttt gcggcttcca
      961 gggcatgata agtaataatc aaatctgcgc ccgcgcgttt gattccagtc aacgcctcca
     1021 gagtgacttt tgcctcgtcg atccagccat ttgcggcagc cgctttgacc atggcgtact
     1081 ccccactgac attgtaggcc gctaacggca cttcgggata catgtccttc accacccgaa
     1141 tcacgtccaa ataagccagt gcaggtttga ccatcagcat gtctgcacct tcctgcacat
     1201 ccaaagcagc ctcacgcagc gcttcatgca cattggcagg gtccatttga tgggacttgc
     1261 ggtcgccaaa tttcggcgcg ccttctgctg cctcacggaa cggtccatag aacgacgatg
     1321 catatttcac cgcgtaagat aagattggca gatgctcata tcccgcgaga tctaatccct
     1381 cacgaatgga gaagaccatt ccatccatca tcccgctcgg cgcgaggata tccgctccag
     1441 attcggcgtg tgagaccgcc accttcgcaa gtacatcaag agtttcatca ttgagtacat
     1501 agccttcagg caagcctgca tgaaaatgct caccggtatt caaaatccca caatgcccgt
     1561 gatcggtata ctcgcacagg catacatccg ttatgacaac catctcgggg atttccttct
     1621 tgatggcgcg aatcgcctgc tggacaattc catcataagc gaaattctcc aatccaatag
     1681 gatccttccg attggggatt ccaaaaagaa tgacggcgct cacaccggac cttgccgcgg
     1741 cttcagcctc acgaacactc tcctccacag acagctgatg gatccctggc atcgattgaa
     1801 tcgcgctgcg acccactcca tgccgcacaa agagcggata aataaaatca cgcgcgttga
     1861 gttctgtctc gcgcagcatg gctcgcaatg caggagtagt acgcaaacgc cggggacgac
     1921 cgcgaagcgc aacggacgat agactagttc tggttttgag ttcaagtaga gtcatcgtgc
     1981 ctccaggtga gctacacacg cgcaagcgcg cacgcagatc agtagagata tgtgtacgtg
     2041 tttatgtgtg cacttgtgta tgtttactga tttcctcaat caatccctcc atcatgtatt
     2101 cttttgccat aacaacgttc gacaatcctt gttcacgcgc ggctcgctct gtgatcggac
     2161 caatacatgc aaataatgga tcacccggaa gcctgaaagg gtcaagtcca tttttctgaa
     2221 cgacagcaat aaaattctgg accgtggaag ggcttgtcaa ggtaacgatt tctacacccg
     2281 cgcgcaacgc atccaacgca atagagtcag gcgtatttag taccgtgcga tagacaacta
     2341 tttcagtgac aatgccacgt gcgttacgaa tctcatttgc cagcactgta cgcgccagat
     2401 tcgactgagg aaatagaaaa cgctttccat aaactttatt ccctaatcct gcggatattg
     2461 cttccgatag gtattcctcc ggcaagtagt caacctggat gccatattca gagattcgtt
     2521 gagccgtctt cgctcccacc gcggcaacat ttaatccgac cgggattttt ttgatcccaa
     2581 ggaaatcaag gcgcttgaag aatgcatcaa cgccgtgtat gctggttagg atcagccaat
     2641 catagtgctt cagacttcgg atcgcaaaat caacgacaga aaaattatca ggaggaacga
     2701 tcttaatcac cgggaagaag atcggttttg cgccaacggc cttcaaggta ttcgcaaatt
     2761 cttcagcctg cgctcgaggg cgagtgatga ggacacgctt atttgtcaga ttcattatga
     2821 aggcagggaa gtaatcaatt gtttatcagc aacggataac gagaggcatc ggacggcgct
     2881 gattcgttcc gcagatgacg tgtgggctga tcaagaattt ttttaacgag tgactgagtt
     2941 agagcctcga tacgtttacg ctcagcatcg gtgagattag gcatatggcg aaaagttttt
     3001 tgtaactcag cagcgcgaat cgtctcggct tgctcgtgca tctctgcgat cattggcaga
     3061 acggcaagtg agtttaggaa ttgcctgaat agtgaggctt cttcgtcgag gatttgctga
     3121 acgcgcggca cttcattcat gcggctggct aaagagcttt ccagctgctc attcaaacca
     3181 tcgatatcaa acagtttgac atgcggaata cccgcaacac ttggatcgat atcccgcggt
     3241 acagcaagat cgatcaacac caacgggcga tgtgtgcgcg tctccatggc ttgcctgacc
     3301 atttgcttcg taataacaaa ctgcaacgcg ctcgtggatg agatcaaaat atccgtctca
     3361 cacaatgcct gctggagaaa ttcaaacggg acggcttccg cattccacag ccgagctagt
     3421 tcctgtgcgc gcgccagcgt gcggttgatc actttgatgt tatccacccc gcgcttgcgc
     3481 aaagcttcaa ccatcaactc ccccgcttcg ccagcgccca ggatgactac actggcagac
     3541 ttgatctgac caagagcctt ttcggcgacc gaagccgcca gggatgacac ggatgccgga
     3601 ttacggctaa tggtagtttc tgccctggca cgcttgcccg catggatcgc tgcctggaat
     3661 aaacgattca ggattggacc tgtcgcatgt tctccacgcg ccaccttcaa tgccctcgtt
     3721 acctgtccaa ga
//
LOCUS       DASTJP010000878         5052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1037029, whole genome shotgun sequence.
ACCESSION   DASTJP010000878 DASTJP010000000
VERSION     DASTJP010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037029"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2430)
                     /locus_tag="VFQ23_25500"
     CDS             complement(<1..2430)
                     /locus_tag="VFQ23_25500"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET9910029.1"
                     /translation="MPIYKYLVHNKTNDPRNGGHLNDASALGFWHLKSIQVQDLYFIE
                     GQLSQEDLQQLALKLLSDPVTQVASWVELPSFLSNPKDDSVLLEVALRPGVTDPVAEQ
                     LVRAAHELNLQGVHRAATGQRFVLCFDESADRIAASQFIAKSLLANNVIQHWTIGEIK
                     PSFPEEADSRGTVEILPVRDLSADRLLAISKARRAALDLAEMQAIQNYFNQEQRDPTD
                     VEFETIAQTWSEHCVHKTFKAKIIKDDGQVIDSLIKTFLKSATEEINAPWVISAFVDN
                     AGIIDFDDEFEVSFKVETHNHPSAVEPFGGANTGVGGVIRDILGVSAKPIANTDVLCF
                     GPQDLNPNSLPEGVLHPRRIQSGVVAGVQDYGNKIGIPTVNGAILYDEGYIANPLVFC
                     GCVGIAPKGKHRKNPQVGDRVILLGGRTGRDGLRGATFSSMTMDAQTGEVSGASVQIG
                     DPITEKGLIDVIVRARDAGLYNDITDCGAGGLSSAVGEMASRLGCDVELRHVRLKYPG
                     LAPWEIWLSEAQERMVLAVSPDDIPALQSLCDTFDTEFTDVGVFTGNGRLVVRYDGSI
                     VVNLDNEFLHEGIPQRTLKAIIEMPVFDARVFEYQISKAKEHLLSLLSSPNVASKASV
                     IRLYDHEVQGGTVVKPLTGIEADAPSDAVVIKPIGTRGTKGIVLANGINPAYGKRDAY
                     KMAWSVIDEAIRNAVAVGADPDRIAILDNFCWGDPLRAETLGTLVEACRGCHDAALFY
                     GTPFISGKDSLNNEYLGIDGLRHSIPPTLLISAIGIVQDVNQSISMDLKEAGNYVYLI
                     GEFSTGQTLVPD"
     gene            complement(2507..3355)
                     /gene="lysX"
                     /locus_tag="VFQ23_25505"
     CDS             complement(2507..3355)
                     /gene="lysX"
                     /locus_tag="VFQ23_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HET9910030.1"
                     /translation="MIRIGFLYTRLRLEEKYLLEELDRQGDVEIIRIKDGDMFFDINE
                     LPEPVDVLFERSISYSRGLYISRIFEAHGIPVVNSSLVAERCGDKYITSQILAKNGIP
                     TPRVLMAFDEESALQAIEAMGYPCVLKPVVGSWGRLLAKIENREMAEALIEHKASLGV
                     HHQVFYIQQYINKPGRDIRAFVVGDEVICAIYRSSENWITNTARGGVATNCPVSDEIA
                     EICQRAARAVGGGLLALDLFETENGLTVNEINHTMEFRNSIDTTGVNIPQRMIEYVLE
                     QVRIKI"
     gene            complement(3452..3949)
                     /locus_tag="VFQ23_25510"
     CDS             complement(3452..3949)
                     /locus_tag="VFQ23_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET9910031.1"
                     /translation="MGKAWKFGDNLNTDEIIPARHNITILPQELAKNVFCEIKPEYAK
                     TVGVGDVIVGGQNFGCGSSREHAPIAIKGSGAVCIIACSYARIFFRNAINVGLPIFEC
                     PEAVADIREGDEIEVNLSTGEIFNYTSGHRYQARPLPDFVLKIAEAGGIVNFLKSHDM
                     EELLA"
     gene            complement(4036..>5052)
                     /locus_tag="VFQ23_25515"
     CDS             complement(4036..>5052)
                     /locus_tag="VFQ23_25515"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HET9910032.1"
                     /translation="DFMKEQGIQQFYHQGICHQVLIEEGYIQPGRVLIGGDSHSNTYG
                     AFGAFGTGFGSTEIGVAWITGKAWFRTPETILIQLEGQTRSGVYAKDVMLYITGRLGM
                     DGATYKSVEFTGSYVQALPMHERIIFANMSTEIGAKCGLIAPDETTIHFLETETKARG
                     PFEMITAVDPNYERMIEIDVTEIGPQVACHPDVDHVRALEQVEGLPIDEVFIGTCTNG
                     RYEDLEIAAQILRGNRVSPFTRTIITPASVQIYDKAMKNGLIQIFHDAGCTIGVVGCG
                     ACIGRHGGLLGPGERAFTTMNRNFIGRMGSPEAEIYLGSPAAAAATAIAGKITDPRDY
                     LENL"
BASE COUNT         1213 a         1369 c         1222 g         1248 t
ORIGIN      
        1 atccggtaca agtgtttgcc cagtagaaaa ttcgccaatc aaataaacat aattgcctgc
       61 ttccttcaaa tccatgctga tggattggtt tacatcctgt acgatgccaa ttgcggaaat
      121 caacaacgtg ggcggaattg aatgtcgcag cccatctata cccagatatt cgttgttgag
      181 cgagtctttg ccgctgatga agggtgtgcc gtagaacagc gccgcgtcat ggcagccgcg
      241 acaggcttcg acaagcgtac ccagagtttc agctcggagc ggatcgcccc agcagaaatt
      301 atcaaggatg gcgatcctgt ccgggtccgc gcccacggcg acggcattgc ggatcgcttc
      361 atcgatcacg gaccatgcca ttttgtaggc gtcgcgtttc ccataggcgg ggttaatacc
      421 attggcgagg acgatccctt tcgttccccg tgtgccgatt ggtttgatga ccactgcatc
      481 cgacggggcg tcggcttcga taccggtcag aggcttgaca acagttccac cctgcacttc
      541 atggtcatag aggcggatga ccgaggcttt actggcaacg ttgggagaag agagaagtga
      601 aagcaaatgc tctttggctt tcgatatttg atattcgaat actcgagcat cgaacacagg
      661 catttcgatg atcgccttca acgttctttg cggaattcct tcatgcaaaa actcattatc
      721 cagattcaca acgatgcttc catcatagcg aacaacaagt cgtccattcc cagtgaatac
      781 accgacatcg gtgaattcag tatcgaaggt atcgcacagg ctctgcagag ccgggatatc
      841 atcgggtgag acggcgagca ccatacgctc ctgtgcttcg gaaagccaga tctcccaggg
      901 agcgagcccc gggtacttca agcggacatg ccttagctcc acatcacagc cgagtctgga
      961 tgccatctcc cccacggccg atgacaggcc gcccgcgccg cagtcggtga tgtcattgta
     1021 caggcctgca tctcttgcgc gcacgattac atcgatcaag cctttttccg tgatggggtc
     1081 cccaatttgg acagatgcac ccgagacttc acctgtttgt gcgtccatcg tcatggaaga
     1141 gaaggttgca ccgcgcaggc catcgcgtcc tgttcgcccg cccagcagaa tgacacggtc
     1201 tcccacctgc ggatttttgc ggtgcttacc tttcggggca ataccgacac atccacagaa
     1261 aaccagtggg ttggcaatat agccttcgtc gtacaaaatc gcgccattga ccgtggggat
     1321 accaatcttg ttaccataat cctgcacgcc cgcaaccaca ccggactgaa tgcgacgtgg
     1381 atgtaaaacg ccctcaggca aggaatttgg gttcagatcc tgcgggccaa agcacagaac
     1441 gtcggtattg gcaatcggtt ttgcagagac acccaggata tcgcggatga ccccaccgac
     1501 accagtattc gcaccaccga atggctccac ggcagatgga tgattgtgtg tctccacttt
     1561 gaatgagact tcgaactcat cgtcaaaatc aataatgccc gcgttatcca caaatgctga
     1621 gataacccag ggtgcattga tttcctctgt ggctgatttt agaaaggtct tgatcagcga
     1681 gtcgatgact tgcccatcat cctttatgat tttcgctttg aaggttttgt gaacacaatg
     1741 ttcgctccat gtctgggcaa tggtctcgaa ttcgacgtca gtcgggtcgc gctgttcttg
     1801 attgaaataa ttttgaatgg cttgcatctc tgccaggtcc aaagcggcgc ggcgtgcttt
     1861 cgagatagcc agcaatcgat ccgcagataa atcacgaaca ggaaggattt ccactgttcc
     1921 gcgggagtcc gcttcctccg gaaaagatgg cttgatctca ccgatcgtcc aatgctggat
     1981 gacgttattt gccagcaaac ttttcgcgat gaattgcgaa gcagctatgc gatctgcact
     2041 ttcatcaaag cacaaaacaa aacgctgacc ggtagcagca cgatgtactc cctgtagatt
     2101 caattcgtgc gccgcgcgga ccaactgctc ggcaacagga tcggtaacgc cggggcgaag
     2161 ggccacctcc agaagaacag agtcatcctt cgggttggaa agaaaggaag gcaactcaac
     2221 ccaggaagcg acctgggtca cagggtcgct taaaagtttg agagccaact gttgcaagtc
     2281 ttcctgcgac agttggccct cgataaagta gaggtcctgc acctggatac tcttcaggtg
     2341 ccaaaatccc agcgcgctgg catcgtttaa atgacctcca tttcttgggt cgttggtttt
     2401 gttatggaca agatatttgt agatgggcat gatgaaccct gtggaaatca acggtattct
     2461 aatcctatgt atttgtctag tcaacaggta attccttcca ttcattctat atcttgatcc
     2521 tcacctgttc caaaacatat tcgatcattc tttgcggaat gttcacaccg gttgtgtcga
     2581 tactgttcct gaattccatt gtgtgattga tctcgttgac ggtcaagccg ttttccgttt
     2641 caaacaaatc aagcgcgagc aatcccccac ccaccgcgcg cgctgcccgt tgacagatct
     2701 ccgcgatctc gtcgctgaca gggcaatttg ttgcgacacc gccgcgcgcc gtatttgtga
     2761 tccagttttc tgaggaacga tagatagcac agatcacttc atcgcccact acaaatgccc
     2821 ggatatcacg tccgggtttg ttgatgtatt gctggatgta aaacacctga tgatggactc
     2881 ccaggctggc tttgtgttcg atcagcgcct ctgccatttc acgattctcg attttcgcaa
     2941 gcaggcgtcc ccaggaaccg acgacaggtt tcaatacaca gggataaccc atcgcttcga
     3001 tcgcctgtag agccgactct tcatcaaagg ccatcaacac cctgggcgtt gggattccgt
     3061 tcttcgccag aatctgactg gtgatgtatt tgtcaccaca tcgttccgcc acaagagatg
     3121 aattcacgac cgggatgcca tgtgcttcaa agatacgaga gatgtacaat ccgcgtgagt
     3181 aggaaatgga tcgttcaaac aacacatcca ctggttcggg aagttcattg atatcgaaga
     3241 acatatcgcc atcttttata cggatgattt ccacatcacc ctgcctatcc aattcttcca
     3301 gcagatattt ctcctccagg cggaggcggg tatataaaaa gccaatacga atcattgttt
     3361 ctccttttca tcttggagtc aaagtctttg cacagcatcc ctgtgggacc agactttgaa
     3421 atcccttatc accgttgaac cgcatgatcg atcatgccag tagttcttcc atatcgtgcg
     3481 atttgaggaa gttgacgatg ccgcctgcct ctgcaatctt caacacaaaa tcgggcaagg
     3541 ggcgtgcctg gtatcgatgc ccgctcgtgt aattgaagat ttcgcccgtg gaaagattta
     3601 cttcgatctc atcaccttcg cgaatgtctg ccacggcctc gggacattca aaaatcggca
     3661 gaccgacatt gatggcgttg cgaaagaaaa tgcgggcata ggagcatgca atgatgcaga
     3721 cagcacctga acctttgatg gcaatcggcg cgtgttcgcg cgaggaacca cagccaaagt
     3781 tctggcctcc gacaatcaca tctcccactc caacggtttt ggcatattcc ggcttgatct
     3841 cacagaagac atttttcgca agttcctgcg gaaggatggt gatgttatga cgagcgggaa
     3901 tgatctcgtc tgtgtttaag ttatctccaa atttccatgc ttttcccatg aacttctcct
     3961 tttaattcac cacaaagggc acaaagaaac acgaaggaaa atccaaacct ttgtgtacct
     4021 tagtgggctt tgtggttaaa gattttccaa ataatcccgc ggatcggtaa ttttgcccgc
     4081 gatggctgtt gctgctgcgg cagctggact acccagatag atctccgcct cgggactgcc
     4141 catacgaccg atgaagttgc ggttcatcgt agtgaatgca cgttccccgg ggcccagcag
     4201 tccgccgtgg cgacctatgc aggcgccgca tcccaccacg ccaattgtgc aacctgcatc
     4261 atggaagatt tgaatcagac cattcttcat cgccttgtca tagatctgta cactggcagg
     4321 cgtaatgatt gtccgtgtaa agggactgac tcgattgcct ctcaaaatct gtgcagcaat
     4381 ctcaaggtct tcgtagcgcc cgttcgtgca ggtcccgata aagacttcat cgatcggcag
     4441 tccttccacc tgttccaacg cccggacatg atccacatcg ggatggcaag ccacctgtgg
     4501 accaatttca gtcacatcga tctcaatcat cctctcgtaa ttgggatcca ctgccgtgat
     4561 catttcgaaa ggaccgcgag cttttgtctc tgtttcaagg aaatggatgg tcgtctcatc
     4621 aggggcgatc aacccgcact ttgcaccgat ctccgtggac atgttcgcaa agataatacg
     4681 ctcgtgcatt ggaagagcct ggacatagga gccggtaaat tccacggatt tgtaggttgc
     4741 gccatccatg cctagcctgc ctgttatata cagcatcaca tccttggcat aaacaccgga
     4801 ccgggtttgt ccctccagct ggatgagaat ggtttccggc gtgcggaacc aggctttccc
     4861 tgtgatccac gcaacgccaa tctccgtcga accaaaaccg gtcccaaacg caccaaaggc
     4921 gccataggtg ttgctatgcg agtcgccacc gatcaacacg cgtcccggct ggatataacc
     4981 ttcctcgatc aacacctgat ggcaaattcc ctggtggtag aactgctgga tgccctgttc
     5041 cttcatgaaa tc
//
LOCUS       DASTJP010000879         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_71701, whole genome shotgun sequence.
ACCESSION   DASTJP010000879 DASTJP010000000
VERSION     DASTJP010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71701"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFQ23_25520"
     CDS             <1..238
                     /locus_tag="VFQ23_25520"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9910033.1"
                     /translation="DGDTYMAEHYNAKEPLNEILNELVENIESLINMLRTLPEEAWAR
                     RSRHATLGSGFTLQTWVERSLAHIEEHLESIKKK"
     gene            complement(264..824)
                     /locus_tag="VFQ23_25525"
     CDS             complement(264..824)
                     /locus_tag="VFQ23_25525"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9910034.1"
                     /translation="MKPSSILELDARLSNQMRVAEKPGALRAIAVFFAHSGDSWFWAI
                     GLFALWLSGDSFWKEWALVQLVGISLLAALVIVIKFSVRRSRPEGEWGGIYRNTDPHS
                     FPSGHAARAFLIAALATGLGPGWLASVLWVWAPLVSLARVAMGVHYISDVLAGCVLGI
                     LFALFGLQVYEPLFMWVSGLIGFPLW"
     gene            complement(821..1753)
                     /locus_tag="VFQ23_25530"
     CDS             complement(821..1753)
                     /locus_tag="VFQ23_25530"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET9910035.1"
                     /translation="MDAIVTAGGIPRPEDPLYTYSHGDSKALIDVAGKPMIQWVLDAL
                     GEAKQVDNVIVIGLSPKSGVTCKKPIYFLSNQGRMLANIVTGVNKSLELNKKNKYVLV
                     VSSDIPTIKPEMVDWLVDTCMETKDDLYYGVCTRELMETRFPDSKRTYTHLKGMDVCG
                     SDINISHVSMATEHLEMWEELIGNRKSPLKQASIIGFGTLFSLFLRQLTLEDAVARVS
                     NRIGIKARAITWPYAEPCMDVDKPHQLELLRADLAQQQSKLQTRARAALKKPTKTKTA
                     VSPKRKSTSQVEKLDGSKTVRSKTKPKTKPKLQQ"
     gene            complement(1853..2758)
                     /locus_tag="VFQ23_25535"
     CDS             complement(1853..2758)
                     /locus_tag="VFQ23_25535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910036.1"
                     /translation="MKRAVSISIGSSKRNKAVEVTLLGEKVSIERIGTDGDMEAAALK
                     YQELDGKVDAFGVGGADLSALVDGKSYPFYSVMPMVRFVKKTPLVDGGGLKNTLENKA
                     ASFLDKQIGDYVNSHGRKVLVALGVDRWGLSKSFADAGYETIFGDLMFGLDIPIAIRK
                     ISQVKTLAALLMPIAGRLPFAWVYPTGEKQEKRTPKWEKYYEWATVIAGDCHYIKRCM
                     PDNLQGKVIVTNTTTTEDVEAFRKAGVKYLFTTTPVLDGRSFGTNMMEAALVAVSGKG
                     RPLSWPELTEIIDRLGFEPQMQELN"
     gene            complement(2802..>4430)
                     /locus_tag="VFQ23_25540"
     CDS             complement(2802..>4430)
                     /locus_tag="VFQ23_25540"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9910037.1"
                     /translation="ADAVPYYLDYQKNHYPFIGEGIRAMTGYGADEIKPHVWGDLVLE
                     TIMLGEADGLSAREAIQLARRGKLKAWKCDQKIRTRDGQIRWLTDRSIELINEQNISY
                     GSIGILQDITDRKSTEADLRKREAILEAIAFAAEQFLKTADWRENIDVVLERLGKTIN
                     ATHAYLFEDHTNLQGDLVTSMTYEWTASGHRSYLNTPYFQNSKVHQDGYEEQVEALSR
                     GELRVGTSSTFNPVERESMDEFGLKAILELPIFVNEKEWGAIGFDDFEQEREWSNAEM
                     GALKIAAGILSAAIKRQLDEDALQRELIERKKLIGELETKNSELERFTYTVSHDLKSP
                     LVTINGFLGYLEQDAASGNMKRLSRDRYRIQEAVNKMQQMLNELLQLSRVGRIINDSE
                     NVPFADLLREAIEISYGRLEARGVTVQINSGLPDVYVDRPRLIEVLQNLLDNAAKYMG
                     DQSHPRIEIGHSGIEAEKPVFYVRDNGIGIATEYHERIFGLFNKLDTKSEGTGVGLAL
                     VKRIIEVHGGRIWVESEAGTGTTFFFTLPTKPAA"
BASE COUNT         1106 a         1195 c          952 g         1177 t
ORIGIN      
        1 tgatggcgac acctatatgg cggagcacta caatgccaaa gagccattaa atgaaatatt
       61 gaatgagctt gttgaaaaca ttgagtcgtt aataaacatg ctacgaacgt tgcccgaaga
      121 agcatgggcg cggcgttccc gccatgccac cctgggaagc ggctttaccc tgcaaacgtg
      181 ggtggaacgc agcctcgcac acattgagga acacctggaa tctataaaaa agaaatagaa
      241 atcatcttat ttcttttcag ggtttaccaa agcggaaatc ctattaagcc tgaaacccac
      301 atgaacagcg gttcataaac ttgtaatccg aacagagcaa acaggattcc taacacgcag
      361 cctgccaaca catctgaaat gtaatgcact cccatcgcca ctcgtgccag tgaaacgagt
      421 ggtgcccaaa cccacagcac actcgccaac cagccagggc caagcccagt cgccagggcc
      481 gcaatcagaa acgcgcgggc cgcgtggcca gatgggaacg aatgaggatc tgtattccga
      541 taaatgcccc cccattcgcc ttcgggtcga ctcctcctca cgctaaactt aataacgatc
      601 accaaagctg ccaataagct gatgcccacc aactgtacga gagcccattc cttccaaaac
      661 gaatctccgc ttagccataa tgcaaataag ccgatcgccc agaaccacga atcccctgag
      721 tgtgcgaaga aaacggctat agcgcgcaat gcgcctggct tttcagccac gcgcatttgg
      781 ttagacaaac gtgcatccag ttcaagaatc gacgaaggct tcattgttgt aattttggct
      841 tcgttttggg tttggttttg ctccgaacgg ttttcgatcc atcaagtttt tcaacctgac
      901 ttgttgattt ccttttcggg ctcaccgctg tttttgtttt ggtcggcttt ttcaaagctg
      961 ctctggctcg agtttgaagt ttactttgtt gttgtgccag atctgcgcgc aataactcaa
     1021 gttgatgagg cttgtccaca tccatgcagg gttccgcata gggccatgtg atcgcgcgcg
     1081 ctttgatgcc aatccgattc gatacacgtg caacagcatc ttccagcgtt aattgcctca
     1141 gaaaaagaga aaagagagtt ccaaagccga tgatgctggc ttgtttgagt ggactctttc
     1201 ggttgccgat caattcttcc cacatttcca gatgctcggt agccatgctc acatggctaa
     1261 tgtttatgtc tgagccacac acatccatcc ccttgagatg agtataggta cgtttcgaat
     1321 ccgggaatcg ggtttccatg agttcacgtg tgcaaactcc atagtaaagg tcgtccttgg
     1381 tctccatgca ggtatccacc agccaatcta ccatctcggg ttttatagtc ggaatatcgg
     1441 aagaaacaac aagtacatat ttattcttct tgttcagctc aagtgactta tttacgcctg
     1501 tcacaatgtt tgcgagcatg cgaccctgat tagaaagaaa gtaaatcggt ttcttacaag
     1561 tcactccact tttcggtgaa aggccgataa caatgacgtt atctacttgc ttagcctcgc
     1621 ccaacgcatc tagcacccac tggatcatgg gcttaccagc gacatcgatc aaggctttcg
     1681 agtcaccatg ggaataggta tacagtggat cttcaggtcg tgggattcca ccagcagtta
     1741 caatcgcatc catacattct cctcatcatc atttcattgc gtcacgcctg cccttgcagc
     1801 tgcacacttg ccgcgcacac aagtatcaag ctaaggccag cgcaatggct tgtcagttta
     1861 gttcctgcat ttgcggctca aagccaagtc tatcaattat ttctgtcaat tcgggccagc
     1921 ttaatggccg ccctttccct gaaacagcca ccaaagccgc ctccatcata tttgtgccaa
     1981 aggaccgtcc atccaaaact ggcgtcgttg taaataaata ctttacgccg gcttttcgga
     2041 aagcctctac atcttccgtc gtagtagtat ttgtaacaat aactttcccc tgaagattat
     2101 ctggcatgca gcgtttgatg taatgacaat ctccggcaat tacggtagcc cattcgtaat
     2161 atttctccca tttgggtgtg cgtttttcct gcttttcacc cgtagggtac acccacgcaa
     2221 aaggtaatcg tcctgcaatg ggcatgagga gcgcagcaag tgtcttgacc tggctaattt
     2281 tacgaatggc aatcggaata tccaaaccaa acattaagtc gccgaagatg gtttcgtagc
     2341 cagcatctgc aaatgacttc gaaagtcccc agcgatccac gccaagtgca accaggacct
     2401 tgcgtccatg cgaattgacg taatcgccaa tttgcttatc gagaaatgag gcggctttgt
     2461 tctcgagtgt gttcttcaac ccgccgccat ctaccagcgg ggtctttttt acgaaccgca
     2521 ccatgggcat gactgaataa aaaggatagg attttccgtc tacgagtgcg ctcaggtccg
     2581 caccaccaac accaaaagca tctactttcc catccagttc ctgatacttc aatgcggcgg
     2641 cttccatatc cccatccgta ccgatgcgtt cgatgctaac cttttcaccg agcagagtca
     2701 cttcgactgc tttgttccgt tttgacgaac cgatgctgat gctgactgcc cgcttcatgc
     2761 gcgattactc ctcacaggga atagctgatt atagtacgga atcaggcagc aggcttcgtc
     2821 ggaagtgtga agaagaatgt ggttcccgtt cccgcttcgc tctcgaccca gatccgacct
     2881 ccatgtacct cgataatacg ttttaccagc gctaacccga cacctgtgcc ttcactcttt
     2941 gtgtccagtt tgttaaacaa gccaaagatc cgttcgtgat actcagtcgc aatcccgatg
     3001 ccattgtctc tcacatagaa taccggcttc tctgcttcaa taccagagtg tccgatctca
     3061 atgcgcgggt gcgattgatc acccatatac tttgccgcat tatcaagcag gttttgtaga
     3121 acttcaatca ggcgcggccg atccacgtaa acatccggta gacccgaatt gatttgaaca
     3181 gtgacaccgc gtgcttcgag gcggccatac gagatttcga ttgcttcgcg caagagatcc
     3241 gcaaatggga cattttctga atcattgatg atacggccga cgcgtgaaag ttgaagcaat
     3301 tcgttgagca tttgctgcat tttgttaact gcttcctgaa tacgatacct gtcacgcgag
     3361 aggcgtttca tattccctga tgcagcatcc tgttcaagat aacccagaaa accgttgatg
     3421 gtcaccagcg gagatttaag gtcgtgtgaa actgtgtacg tgaaacgctc aagctcactg
     3481 tttttggttt ccaattcccc gatcagcttc ttgcgctcaa taagctccct ctgcagggca
     3541 tcttcatcta attgacgctt gatcgccgca ctgagaatcc ccgccgcaat cttcagtgcg
     3601 cccatttcag cattgctcca ctcgcgttcc tgctcaaaat catcgaagcc aatggcgccc
     3661 cattcttttt cattgacaaa gatcggtagc tccaaaatcg ccttgagacc gaattcatcc
     3721 atgctttctc tttcaaccgg attaaatgtg gaagaagtcc caacacgcaa ctccccgcgg
     3781 cttaacgctt ccacttgttc ttcataccca tcctggtgga cttttgaatt ctgaaaatat
     3841 ggagtgttca gataacttcg atggccagag gccgtccact cgtatgtcat ggaggtaacg
     3901 agatcccctt gtagatttgt atgatcttca aaaagatagg catgagttgc attgattgtt
     3961 tttcccagac gttcaagaac cacatcaata ttctcacgcc aatcagctgt tttgagaaat
     4021 tgttctgcgg caaatgcgat tgcctccaaa atggcttcgc gttttcgcag atcggcttcc
     4081 gttgatttac gatctgtaat atcctgcaga attccaatcg aaccgtacga aatattctgc
     4141 tcattgatca attcgatgga cctgtcggtc agccagcgaa tttgaccatc gcgcgtcctg
     4201 attttttgat cacacttcca ggctttgagc ttgccgcgcc ttgcaagctg gatcgcctcc
     4261 cgtgcgctca acccatctgc ctcgcccagc atgatcgtct ctaatacaag atccccccac
     4321 acgtgaggct ttatttcatc cgcgccataa ccggtcatgg cacggatccc ttcccctata
     4381 aaagggtaat gatttttttg ataatcaaga taatacggta cggcatctgc
//
LOCUS       DASTJP010000880         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1037222, whole genome shotgun sequence.
ACCESSION   DASTJP010000880 DASTJP010000000
VERSION     DASTJP010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037222"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..757
                     /locus_tag="VFQ23_25545"
     CDS             293..757
                     /locus_tag="VFQ23_25545"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9910038.1"
                     /translation="MNTPIVRNWMTVNPITITPQTTLPNVRRLMHDHHLRLLPVLNNG
                     NLVGVVTWRDIHQAETWDGVDFGGPEQNSLLEHLTAKEFMSYRPLMITPDLTLDQAAY
                     LMVDHKIGGLPVVEFGRLVGVITETDICRSMLQWKIVVSNSEPILDTPLENA"
     gene            786..1211
                     /locus_tag="VFQ23_25550"
     CDS             786..1211
                     /locus_tag="VFQ23_25550"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910039.1"
                     /translation="MLGSLKTSFIGYLTFRGGEGQISFLLHRITGLGLLLFLTIHILD
                     TALVYFAPHLYEHALELYRSTPFMLGEIGLVFCVLFHGLNGLRLAYIDLFANAVWDVK
                     RFGRSVWITFIITLILWIPAAIVMVYKLLYYNYGFLSGK"
     gene            1223..1612
                     /locus_tag="VFQ23_25555"
     CDS             1223..1612
                     /locus_tag="VFQ23_25555"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910040.1"
                     /translation="MFILRKAKIKRNYEIVAWKWMRYSAIILIPLVWVHVLIQDILVG
                     AHRITLDYVEQRWSMIGWQIYDILLLSFAFAHGMNGLRQVMVDHIHGRKARSICGWLI
                     FLAWVAISLIGGLAIVGAAHKHLAALY"
     gene            1878..2297
                     /locus_tag="VFQ23_25560"
     CDS             1878..2297
                     /locus_tag="VFQ23_25560"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9910041.1"
                     /translation="MNTQIVRNWMTPHPITISPQTTLAEAKNLMVNYHLRRLPVVDKD
                     RLVGMVTWRDINRAESSVSSTLRPYEISLLRTQLTAREFMSTALVTISPDATVEESAG
                     LMIEHRISGLPVVENGKMVGLITETDICIYVMQLEAV"
     gene            2304..2435
                     /locus_tag="VFQ23_25565"
     CDS             2304..2435
                     /locus_tag="VFQ23_25565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910042.1"
                     /translation="MKKLEMSQQRSGILVLISALIVLILLPFAWRSFLKKISAQKCF"
     gene            2722..>3078
                     /locus_tag="VFQ23_25570"
     CDS             2722..>3078
                     /locus_tag="VFQ23_25570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET9910043.1"
                     /translation="MNFKMWHSALRVIPRVSVEEWSSLDFVSKWLVSSRAAVLVMTLI
                     SGILAGLFAIRDNLFNSVPWFVLVMGLIMAHAGNNFLNDYTDYVRGVDQKDYYRALYG
                     PQPLVDGLMTKTQNITY"
BASE COUNT          793 a          694 c          748 g          843 t
ORIGIN      
        1 gtgtataaag caagcaagat ttttgacatt taaatatcct ttctatactt tggtaaagtc
       61 tatcccgcca ctccatgttc ctctgaaaca gagatgctca tcggatctcc tgtcgcatta
      121 aggattagtg ttgccaattt caggaaacga ttaaatcggc attcatagcc tttgcataca
      181 ttatcgcgct acaggtatgg atcataaggg tcatccgccc tataaagagc gggatacttg
      241 ccccgtgacg atgggggagg ggatagagat aatgagcatg gaggatcagt ctatgaatac
      301 cccaattgtt cgcaactgga tgacagtcaa tccaattacc atcacacccc aaacaactct
      361 accgaacgta cgccgactta tgcatgacca ccacctccgt cttcttccgg tactaaacaa
      421 tggcaatctc gttggtgtag tgacctggcg tgatatccac caggcagaaa catgggatgg
      481 cgttgacttt ggtggacctg aacagaactc tttgctcgag cacctgacag ccaaggagtt
      541 tatgtcttat cgccctctga tgattacacc ggatctcact ctcgaccagg ccgcttactt
      601 aatggtagac cataagattg gtggcctacc agttgtggaa tttggcaggc tagttggcgt
      661 gattactgag acagacattt gtcgatcgat gttgcagtgg aaaattgtcg tatccaactc
      721 ggaacctatt ttggatacac cattggaaaa tgcgtaagac gtaggagcag aatcaggagg
      781 cgttgatgct cgggagcctt aagacctcgt ttatcgggta tcttacattt aggggtggcg
      841 aagggcagat ttccttcctt ttgcatcgga taaccggact cggtctttta cttttcctga
      901 ccatccacat cctcgataca gctttggtgt actttgcgcc tcacctctat gaacatgcac
      961 ttgagttgta tcgctccaca ccttttatgc tgggtgaaat cggattagtg ttctgcgtgt
     1021 tgttccatgg tctgaatggg ctgcgccttg cctacattga tctcttcgcc aacgcggtgt
     1081 gggatgtcaa aaggttcggt agatccgtat ggatcacatt tatcattacc ttaattctgt
     1141 ggatccctgc tgcaatcgta atggtataca aactccttta ctataattac ggttttcttt
     1201 ccggaaaata gagtacaacc tgatgttcat acttcggaaa gcgaaaatta aacggaacta
     1261 tgaaatcgtc gcctggaaat ggatgcgtta tagcgcgatc atattgatcc cgttagtgtg
     1321 ggtgcatgta ctgattcagg atattctggt tggcgctcac cgtattacac tggattacgt
     1381 tgagcagcgt tggagcatga taggttggca aatttatgac attctcttac ttagttttgc
     1441 attcgcacat ggcatgaacg gtttgcgtca ggttatggta gaccatatcc atggcagaaa
     1501 agcccgaagc atctgtgggt ggcttatctt cctcgcctgg gttgccattt ccctgatcgg
     1561 aggacttgca attgtcggtg cggcgcacaa acatttagcg gcactctatt agattcgaca
     1621 cagaatattt tgttctccag ggtaatggaa ttagttcgac aaacaagagt agtgatgtaa
     1681 tcgcaaaaga cgttcaagat ggatttaatc aggggcgaca ggtcaggact ctccaactgc
     1741 tattggatcg tagcctcata agcaaccgcc aataggcagg gcggagggtc atgggtcatc
     1801 cgtcctaagg aaaacgggat acttgcccga tgacgatacg ggcgtacgtg gagataattt
     1861 gtatggagga ttactctatg aacacccaaa tcgtacgaaa ttggatgacc cctcacccga
     1921 ttactatcag ccctcagacc actttagctg aagccaagaa tctcatggtt aactaccatc
     1981 ttcgccgtct tccagtggtc gataaagaca ggcttgttgg aatggtcacc tggcgtgata
     2041 tcaaccgagc ggaatcgtca gtcagcagta ctttgcgtcc gtacgaaatt agcctcctgc
     2101 ggactcagtt gacagcacgc gaatttatgt ccaccgctct tgtcacaatt tcaccagatg
     2161 ccactgtcga agaatccgcc ggcctgatga tagagcaccg aattagcgga ttgcctgttg
     2221 tggaaaatgg caagatggtt ggcctgatca ctgaaacgga tatctgcata tacgtcatgc
     2281 agctggaagc tgtctaatga atcgtgaaga agcttgagat gagccaacag cggagcggga
     2341 ttctggtcct gatctcggcg ctgatcgtct taatcctcct gccattcgcc tggcgcagct
     2401 tcctgaagaa gatttctgct caaaagtgtt tctaaatgca gtatttgccc aagctcaatt
     2461 atccaaaatt tcaaatttcg aaggatgagt actatgcaaa ttatgcgcca agctgacttt
     2521 acagcacgcg ctgtgctgca tttagcccgg gcagaaaata gagagtaccg ttccaccggc
     2581 tctgctgcaa tatccagaaa aaaagaactg cgaaactgaa aaatgcaaac cttgctcaat
     2641 ggatcgcacg acaatccttt tgtgatgagg aagaatgctg accttcatcg aaatatggtg
     2701 gaaacggaag gagcatagtg catgaatttt aagatgtggc atagtgcttt gagagtgatc
     2761 cccagggttt cagtggaaga atggagtagt ctcgatttcg tatcaaaatg gctcgtgtca
     2821 agccgggcag cggtgctggt tatgacattg atctccggga ttcttgccgg gctctttgca
     2881 attcgcgaca accttttcaa ttcggttccc tggtttgtgt tggtcatggg tctaatcatg
     2941 gcacatgccg gcaacaattt tctcaatgat tatacggatt acgtgcgggg tgtggatcag
     3001 aaggattact atcgtgcttt gtacggcccg cagcctctgg ttgatggact aatgaccaaa
     3061 actcagaata tcacctat
//
LOCUS       DASTJP010000881         7232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1037256, whole genome shotgun sequence.
ACCESSION   DASTJP010000881 DASTJP010000000
VERSION     DASTJP010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037256"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFQ23_25575"
     CDS             <1..461
                     /locus_tag="VFQ23_25575"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET9910044.1"
                     /translation="DKGRTFEEGVTILSAQFPQYADLIRAYHENWVDSIGESFTGTVE
                     ILKRLKKAGYPLYGFSNWSAETFPYARAKYDFFELFDDMVISGEVGFVKPEPEIYQVL
                     LNKVGRPADECLFIDDSLPNIQQANKIGFATIHFQSPSQLEEELKQLQLI"
     gene            480..1502
                     /locus_tag="VFQ23_25580"
     CDS             480..1502
                     /locus_tag="VFQ23_25580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9910045.1"
                     /translation="MSIIHIKELNKHFKILNRREGLGGAFRDLFSGDYRTVEAVSGIS
                     FDIDAGEIVGYIGPNGAGKSTTIKMMTGILKPTSGVIQVNGQVPYDNRIRQAQIMGVV
                     FGQRTQLWWDLPVIESFRILKEIYKVDQKTFDQHMGLFNDLVGLNALYPQQVRTLSLG
                     QRMLCDITASFLHNPQVVFLDEPTIGLDISVKAKIRSVIQELNRERNTTIILTTHDLG
                     DVEALCHRIIIIDKGKILYDGDIKKVNALFGAYRTIKLQINDFNENTLASLKNKLSDR
                     FGSENEISIAQTEEFWTDVTIDQARTPLSDVLGFVMSSFKVNDVRILEISMENVVRKV
                     YDGALG"
     gene            1499..2290
                     /locus_tag="VFQ23_25585"
     CDS             1499..2290
                     /locus_tag="VFQ23_25585"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9910046.1"
                     /translation="MRTYLPIARATYMIGLVYRFGFLFAILGNFIYLGVAYYLWRSIY
                     RYSETLRGLTFNEAFLYVALGSSIFILLKTYVDWYIHQEIREGTIAIYLTKPVDYQIY
                     TLFANFGSVLINLTSITIPTILMLLFVFKVKIALGAGLVLFPISLLLAFIVSFSLDYF
                     VGLLGFYSESVWGLSITKEIIVSVLSGALIPLQFFPEAIQRVLLWLPFQAIYHTPIMM
                     ITRADLGVGTFIPMLLIQLMWAVVLFVLVRLFYNQAIKVLRIAGG"
     gene            2293..3090
                     /locus_tag="VFQ23_25590"
     CDS             2293..3090
                     /locus_tag="VFQ23_25590"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET9910047.1"
                     /translation="MRTVGEYIRLYFIIEAQYIKARMQYRADFIISSIGMFFSSLVTL
                     GVFWVILESVPDLAGWSLMEMVFIYAFYMIAISPMQILFDHAWNIRFHIRQGTFLKYY
                     FRPLNMMFYYMSEMFDLKGLTQLFMGIALLIYASVQLNIDWTLTRFILLLITLFSASL
                     VQISIVVAASCAAFWLVESYPVLGLAWKLREFSPYPMTIFDGAFRFAFTYIIPIGFVA
                     FYPSQLFLRPEEVAPLIYFSPIVGIGLFAITYWIWTKGVNSYTGTGS"
     gene            3186..4187
                     /locus_tag="VFQ23_25595"
     CDS             3186..4187
                     /locus_tag="VFQ23_25595"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9910048.1"
                     /translation="MDLKPLLADIELELQKQISRLDTPRTKFFHEMLTYHMGWTGEGA
                     GPEATGKRIRPLMVLLSTAASGGDWKLALPAAAAIELVHNFSLVHDDIQDNSPTRRSR
                     ATAWVKWGAPMAINVGDALFVIANQAIIDLKQSYPADVVLRAAEILNNTCLELTRGQY
                     LDISYEERTDLTVEDYWPMISGKTAALLSACCHIGSLLGGVDDSKQEAYRSFGHYLGL
                     AFQVQDDILGIWGDEAVTGKSAASDLVEGKNSLPVLAGLGRKGQFSARWAKGPIQAEE
                     IEEVKRMLASEGGLIAAEDASKQMTDLALMSLREADPQGAAGDALSKLADTLLKRKQ"
     gene            4616..4687
                     /locus_tag="VFQ23_25600"
     tRNA            4616..4687
                     /locus_tag="VFQ23_25600"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4648..4650,aa:Gly,seq:ccc)
     gene            4824..5219
                     /locus_tag="VFQ23_25605"
     CDS             4824..5219
                     /locus_tag="VFQ23_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9910049.1"
                     /translation="MAQPANKGTILYVEDNPENRLLVRRILLAENYKLLEAKNAFEAF
                     EVLNSTTPDLILMDINMPDMDGYTLTTKIRSMPGLARVPILALTANVMRGDKEKTLEA
                     GCDGYIQKPLDIDQLTREIEKFLKRRANV"
     gene            5212..5655
                     /locus_tag="VFQ23_25610"
     CDS             5212..5655
                     /locus_tag="VFQ23_25610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9910050.1"
                     /translation="MSENIMDTQPIKKPTIPKDATVLVVEDNVANFVLIARMLGYLGI
                     HCEWKTSGYEVVEYADTLQRLDLILMDIRLPYEDGYGALKKIRASERLKAIPIIAVTA
                     EASTDQMNKAKDSGFDGFLGKPLDPDKFPDQIRRILSGESVWEFS"
     gene            5679..>7232
                     /locus_tag="VFQ23_25615"
     CDS             5679..>7232
                     /locus_tag="VFQ23_25615"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9910051.1"
                     /translation="MISINRNLFFVTVYFTVDGDRLHLAHYSDPQSRDSQATVPRAAE
                     LSLHDIESHLKNGPLRVDLRDASTLPPSLVVYPHQLGCETAAYLPITQNRSLRGLLLI
                     GARAGQDLNDDVINSFATTIQLTTNVLERRPSSPQPLDGRQSIEMNALNTLAANAATV
                     NDLHAFYAAIHEQIRTVVGDYGIVIAIYDQKTNSINIPYLWEEGKFSSIDTFPLGEGL
                     TSILIRTREALMLVEDTEKRSVAMGAKVAGRAAKSWLGVPMVARGEAIGAIIIQDLEQ
                     EHSFDDNDLRFVMAVANQISGALYNIRLLEESRRSALQFETAAEIARDISSSLNLDEL
                     LQKAVNLIRTRFHFYHAGVFLKDFTDEFIVIREATGEAGAQLKRASYKLAVGSKSVIG
                     FVAGRGEVLVVNDTARDVTYHFNPLLPDTKSEAAIPLKIGERTVGVLDVQSQETYAFS
                     EDNLRTLQILADQLAVAVVNTELFAETQEHLAQHRLLHHITTTAASGTTLEEALQSAV
                     NGLQVTLGGD"
BASE COUNT         1851 a         1695 c         1773 g         1913 t
ORIGIN      
        1 aggataaagg tcgcactttt gaagagggcg taaccattct ttcagcacaa tttccacaat
       61 atgccgacct aattcgggct tatcacgaga attgggtcga ttcaatcggt gagtccttta
      121 cgggcacagt agagattttg aagcgactca aaaaagccgg atatccgcta tatggcttca
      181 gcaactggtc ggcagagacc ttcccgtacg cgcgtgccaa atatgatttt ttcgagttgt
      241 ttgatgacat ggtcatctcc ggcgaagtcg gattcgtcaa accggagcca gagatttatc
      301 aggttctgtt gaataaggtc ggcagaccag ccgatgaatg cctgttcatt gatgactcgt
      361 tgccaaatat tcagcaggcg aataaaatag gcttcgcgac cattcacttt caatccccat
      421 ctcaacttga agaagaatta aagcaacttc aactgatatg accgcgtcgg tctctatcca
      481 tgagcatcat ccacatcaaa gaactcaaca agcatttcaa aatcttgaat cgccgtgagg
      541 gattaggcgg cgcgtttcga gacctgtttt caggagacta ccgaactgtc gaagcggtat
      601 caggcatctc gtttgatatc gatgccggag aaattgtcgg atatatcggt ccaaacggtg
      661 cagggaaatc cacaaccatc aaaatgatga cgggtatcct gaaaccgacc agtggcgtca
      721 ttcaagtcaa tggacaagta ccctacgata accgcatccg ccaggcacag atcatgggag
      781 tggtgttcgg tcagcggaca caattgtggt gggacctgcc cgtcatcgaa tcattccgga
      841 ttctgaaaga gatttacaag gtcgatcaaa agaccttcga ccagcacatg ggcttgttca
      901 atgacctggt ggggctcaac gcgttgtatc cacaacaagt gcggaccttg tcccttggac
      961 aacggatgtt gtgcgacatc actgcatctt ttctacataa cccacaggtt gtttttctcg
     1021 acgagccaac catcggattg gatatttcag tcaaagcgaa aattcggtcg gtgattcagg
     1081 aacttaatcg ggagcgcaac acgaccatca ttctgaccac acatgacctg ggggacgtag
     1141 aagcactgtg tcatcgcata attattatag acaaaggaaa aatcttgtac gatggcgata
     1201 tcaaaaaggt caatgcgtta tttggcgcgt atcgtacgat taagcttcaa atcaacgact
     1261 tcaatgaaaa cacccttgcc tcactgaaaa ataagctctc cgaccggttt ggttcagaga
     1321 atgaaatctc aattgcacag accgaagagt tttggacaga tgtgactatt gaccaggcgc
     1381 gcacgcctct ctcggacgtc ctgggcttcg tcatgtcgag tttcaaagtg aatgatgtgc
     1441 gcatccttga aatatccatg gagaacgtgg tgagaaaagt ctacgatggg gcgctgggat
     1501 gaggacctac cttccgattg cgcgcgccac gtatatgatc gggctggtgt atcgctttgg
     1561 atttctattc gcgatcctcg gaaacttcat ttatctgggt gtcgcctact atctttggcg
     1621 aagtatctac cgttattccg aaacgttacg cgggcttacc ttcaatgagg ctttcctata
     1681 tgtagctttg gggagttcca ttttcatatt gcttaagacc tatgtggatt ggtacatcca
     1741 tcaagaaatt cgtgagggga cgatcgcgat ctatttaaca aaacccgtcg attaccagat
     1801 ctacacgctt ttcgcgaact tcggttcagt cctgataaac ctgacgtcga ttacgattcc
     1861 gactattttg atgttgttat ttgttttcaa agtcaagatt gcactcggag ccgggctggt
     1921 gttgtttccc attagcctgt tactggcgtt tattgtcagt ttttcgcttg attattttgt
     1981 tggattattg ggcttttact ccgaatcggt ctggggcttg agcattacga aagaaattat
     2041 tgtgtctgtg ctgtcaggcg cgctcatccc actccaattc tttccggagg cgattcagag
     2101 agttttgttg tggcttccat ttcaggccat ttatcacact cctatcatga tgatcacgag
     2161 ggccgatctg ggagtaggca cattcatacc gatgctgttg atccagctaa tgtgggcggt
     2221 cgttcttttc gtcctcgtgc gtctgtttta caaccaggca atcaaagtat tgcggatcgc
     2281 gggaggctaa acgtgcgcac agtcggcgaa tacattcgtt tgtatttcat cattgaggcg
     2341 caatatatta aagcgcgcat gcagtatcgg gcggacttca tcatcagttc catcgggatg
     2401 tttttttcca gcctggtaac actaggcgtt ttctgggtga tcctcgaatc ggtgccagac
     2461 ctggcgggct ggtctttgat ggagatggtg ttcatctacg cgttctacat gattgccatc
     2521 tcaccgatgc aaatcctgtt cgatcacgcc tggaatattc gcttccatat caggcaaggg
     2581 acatttctga agtattattt tcgtcccctc aatatgatgt tttattacat gtctgagatg
     2641 ttcgacctaa agggattgac tcagttgttc atgggtatag cactgcttat ttatgcttcc
     2701 gtacaattga atattgactg gacgttgaca agatttattc ttttgttgat caccttattt
     2761 agcgcgtcgc tcgtgcagat atccattgtc gttgctgctt cttgtgctgc gttctggctg
     2821 gtggagtctt accctgttct tggacttgcc tggaaattgc gtgagttctc tccctatccc
     2881 atgacgatct tcgatggagc attccgcttt gctttcacgt atataattcc gattggattt
     2941 gtcgcgttct atccttccca gctctttctt cgacctgaag aggtcgcgcc tctcatttat
     3001 ttctccccca tcgtcggcat tggactcttc gccatcacct actggatctg gaccaaaggc
     3061 gtgaactctt atacgggaac aggttcgtga agaaaagaaa cgatcataac ctttggttta
     3121 tatccacctt cagaacgaca aacacactac aggaaatatt cccgccatag gcaggtaaat
     3181 tatttatgga tttgaaacca cttcttgctg acattgaact ggagctacaa aaacagattt
     3241 cgcgcctcga cacgcctcgt acgaaatttt tccatgagat gcttacgtat catatgggat
     3301 ggaccggcga aggcgcggga ccagaggcta ccggcaaacg catccgtcca ttgatggttc
     3361 tgctgtcaac ggcggcctct ggtggagatt ggaagttggc actgcctgcc gcagcggcaa
     3421 ttgaattagt ccacaacttt tcgcttgttc acgatgatat ccaggataac tcacccacac
     3481 ggcgcagtcg cgctacagcc tgggtcaaat ggggcgcgcc gatggcgatc aacgttgggg
     3541 acgcgctttt tgtcattgcc aatcaggcaa ttattgatct aaaacagagt tatccagcag
     3601 atgtcgttct gcgagccgcg gagatattga acaacacctg ccttgaactc acccgcggtc
     3661 aatacctgga tatatcgtac gaggaacgaa cagacctgac cgtggaagac tattggccga
     3721 tgatctctgg caagaccgcg gctttgttgt ccgcttgttg tcatatcggt tcgttgttgg
     3781 gcggagtcga tgattccaaa caagaggcct atcgttcttt cgggcattac ctcgggctgg
     3841 cgtttcaagt ccaggatgac atacttggaa tttggggtga tgaagcagtc accggcaaat
     3901 ctgccgcaag cgacctggtc gaaggaaaaa attcccttcc agtgctggct ggtttaggga
     3961 gaaaaggcca gttttcagct cgttgggcaa aaggtccaat tcaggcggag gaaattgagg
     4021 aggtaaagcg catgctcgcc agcgaaggcg gtcttattgc cgcggaggat gcatccaagc
     4081 agatgaccga tttggccctg atgagcctgc gcgaggcaga tccgcagggc gcggccggag
     4141 atgccttatc gaaactagcg gatacattgc taaaacggaa gcagtgagcg atttcatcgg
     4201 tgtctgacgg tagatgctgt accaaattga aaggtaatag aaaagtcaac aatggaacca
     4261 gtccgtatac aatcatggca tagagaaagg atgtagtcat ggaaccaatt tccttatgcc
     4321 tcgctgtaat ctctacgctg ttcttcagct ggcttgtata taaataccag gaaagaataa
     4381 cggcacgctt tttcctccat agaggaacaa aaacgatcag gaagtcggat tgcattttgc
     4441 tcccgaatat ggcttgttag ccgtggtgct gttttccatg gtctttcttg cagtagtaat
     4501 tggattaacg cttacgttgt aacaattcga ctcaagccgc atgctgatca acttcacgat
     4561 gaaattctga acacttgctc atcctcataa aacagaataa aatggtattc aagatgcggg
     4621 tgtagttcag tggtagaacg cttgcttccc aagcaagata tcgtgggttc gagtcccatc
     4681 acccgctcac ctgtgctggg aaaggtacag gaaaatgtcg aggagagact ccctcgacat
     4741 ttttgtttgg caaattgtaa ggtagctgtc atgcgttcgg acaacagtcg taagtagaat
     4801 cggggctgga ggtacctgtg taaatggcac aaccagcgaa taaaggcacc attctgtatg
     4861 ttgaagataa ccccgaaaat cgactgctcg tcaggcgcat tctgcttgct gaaaattata
     4921 aattgcttga agccaaaaat gcatttgaag cgttcgaggt attgaattct acgaccccgg
     4981 atctgatcct gatggacatt aatatgccgg atatggacgg ttatacatta accacgaaga
     5041 tcagatccat gcccggcctt gcaagagtcc caattcttgc gttgaccgca aatgtgatgc
     5101 gcggcgataa agagaaaacg cttgaggcag gctgtgatgg ttatatacaa aaaccacttg
     5161 atattgatca attgaccagg gagatcgaaa aatttcttaa gaggagagcc aatgtctgaa
     5221 aatatcatgg atacacaacc tatcaaaaag ccaaccattc cgaaagatgc gacggttctc
     5281 gtagtcgagg acaatgtcgc caacttcgta ttaatcgcca ggatgttggg ctatcttggc
     5341 attcactgtg aatggaaaac atccgggtac gaagtggtgg agtatgcaga tacgttacag
     5401 cgacttgacc tgatcctcat ggatattcgg cttccctacg aagatggtta tggtgcgctc
     5461 aaaaagattc gagcctctga aagactcaaa gcaattccta tcattgcggt gactgctgaa
     5521 gcaagcacgg atcaaatgaa caaagcgaag gattctggct ttgatggctt cctcggtaaa
     5581 ccactggatc ccgacaaatt tccagatcaa atccgccgca ttttgagcgg agagtctgtt
     5641 tgggagttta gctaagccgt tgttctgtaa acatgagtgt gatctcgatc aacaggaatc
     5701 tcttttttgt taccgtttac ttcaccgtgg acggcgatcg tttgcacctg gcgcattatt
     5761 cagaccctca aagccgggat agtcaagcga ctgttccgcg cgccgcggag ctttcgcttc
     5821 atgatatcga gagccatctg aaaaacggtc cgctccgggt agatctacga gacgcatcaa
     5881 cccttcctcc atcacttgtt gtttatccac atcaacttgg atgtgaaact gccgcatatt
     5941 tgcccatcac gcaaaataga agcttgcgcg gcttgctctt aatcggtgca cgagccgggc
     6001 aagatctaaa cgatgatgtc ataaattcat tcgccacgac catacaactg acaacaaacg
     6061 tcctggagcg cagaccgtca tcaccccagc cactggacgg acgccaatcc atagaaatga
     6121 atgcgctaaa cacgcttgca gccaatgccg ccacagtaaa tgatctacac gccttttatg
     6181 cagccattca tgagcaaatc cgcacggtgg tcggcgacta tggcatcgtg attgcgatct
     6241 atgaccaaaa aaccaactcc atcaatattc cttatctgtg ggaagaggga aaattttctt
     6301 cgattgatac ttttccttta ggtgaaggct taacctcgat cctgattcgc acgcgggaag
     6361 ctttgatgct agtggaagac acggaaaaac ggtccgttgc catgggcgcc aaggttgcgg
     6421 ggcgagcagc aaaatcctgg cttggagttc ccatggtcgc tcgaggggaa gcgattggtg
     6481 ccatcattat ccaggatctc gagcaggaac atagttttga cgataacgat cttcgatttg
     6541 tgatggcggt cgccaatcaa atttcaggcg cgctctacaa tatccgattg ctggaggaga
     6601 gccgtcgttc ggcactgcaa tttgaaactg ccgctgaaat tgcccgcgat attagcagct
     6661 cactgaacct ggacgaactg ttacagaaag cagttaactt aattcggacg cgatttcatt
     6721 tttatcatgc cggagtcttt ctcaaggatt ttacggatga gttcattgta atcagggaag
     6781 caacaggaga ggcgggcgcg caattgaaac gcgcaagtta taaacttgcg gtgggttcaa
     6841 agtcagtcat cggctttgtg gcaggcaggg gcgaagtttt ggttgtcaac gatacggcac
     6901 gtgacgtaac gtatcacttc aatcccctgc tgcctgatac aaaatctgag gcggcaattc
     6961 cactaaaaat cggggaacgg actgtgggtg tcctggacgt tcagagccag gagacgtacg
     7021 cattttcaga agataatctt cgcacactgc aaattcttgc agatcaactg gcggttgcag
     7081 ttgtcaatac tgagcttttt gccgagactc aggaacacct ggcccaacac cgtctcttac
     7141 atcatattac taccaccgcc gcctccggca cgaccctgga agaggccctc cagtctgctg
     7201 tcaacggatt acaggtcacc ctgggcggag at
//
LOCUS       DASTJP010000882         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_206642, whole genome shotgun sequence.
ACCESSION   DASTJP010000882 DASTJP010000000
VERSION     DASTJP010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206642"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFQ23_25620"
     CDS             complement(<1..655)
                     /locus_tag="VFQ23_25620"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9910052.1"
                     /translation="MNCGTRLAVETEGGGEKPQFKLDRYLPQELITKLEAARSRNSMV
                     GERRVITMLFCDVKGSTAAAEKVDPEVWTDIMNGVFEYMIRPIYKYEGLVPRLMGDAI
                     LAFFGAPITHEDDPQRAVLAGLEIQDGIRSYAEEVRLKHGLEFGLRVGINTGLVVVGE
                     IGSDLRMEYTAIGDAINLAARMEQTAQVGTIQISDETYKLVAPFFDVEALGEVEVKGK
                     "
     gene            complement(966..1946)
                     /locus_tag="VFQ23_25625"
     CDS             complement(966..1946)
                     /locus_tag="VFQ23_25625"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9910053.1"
                     /translation="MKAIIYTKYGSPDVLQFKEVEKPAPGEDEVLVKIHAAAATIGDV
                     IIVKGEPFVVRFSTGLLEPKHKIPGKEMAGQVEAVGKNVKRFRPGDEVFGDLSVCGFG
                     AFSEYVAVPEKAIALKPANLTFEEAAAVPESSVVALQGLRDKGKIQPGQKVLINGASG
                     GVGTFAVQIAKSFGAEVTAVCSTKNLDMARSLGADHVIDYTREDFTQNGHRYDLILAA
                     NGYHSLSEYRRVLSPGGIYVGTGGTMAQTFEAMLQGPVISMTGSQKMGNMLVKPNQDD
                     LVFMKELIEAGKVMPVIDRCYPLRGVADALRYINEGHARGKVVITLAQNN"
     gene            complement(2051..>2823)
                     /locus_tag="VFQ23_25630"
     CDS             complement(2051..>2823)
                     /locus_tag="VFQ23_25630"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9910054.1"
                     /translation="QRKGDNTMNTKTEVNKYRTTAIVVGVVYLLGFVVGLVGMGLFTS
                     ILSGPGYLSTVSTKSTLLGFGAVLWLMAVIGDAAHGVLMFPVLKQHSERMAAGYLAFR
                     IIDATFVAIMVLFVMIQIPLGSEYLKAAASDAPSLQALSTLFAQIQQYAYQIGMSALG
                     VSGLMLCYTLYKAKLVPGWLAIWGLVGYATIFIGMLSAIMGSGLGDLSSIPGGLWEVF
                     MGVWLIAKGFNSSAFVSLSAKTSTPAESLVPQTNPANS"
BASE COUNT          662 a          780 c          672 g          709 t
ORIGIN      
        1 atttcccttt gacttccact tcgcccagtg cttccacatc gaagaagggc gcaaccaatt
       61 tgtaggtctc atcagagatc tggatcgtgc ccacttgtgc tgtttgctcc atacgcgctg
      121 cgagattgat cgcatcgcca atggcggtgt attccatccg caggtcggag ccgatctcgc
      181 caacaacgac caggccagta ttgattccaa cccgcagacc aaactcaagt ccatgtttga
      241 ggcggacttc ctctgcatac gatctaattc cgtcctgaat ttccaggccg gccagtaccg
      301 cgcgctgcgg gtcatcctcg tgtgtgatcg gcgcgccgaa aaaagcaagg atggcatccc
      361 ccatcaggcg cgggactaac ccttcatatt tatagatggg gcggatcata tactcgaaga
      421 caccgttcat gatgtctgtc cagacctcgg ggtccacttt ttcggcggca gccgtggacc
      481 ctttgacatc acagaacagc atcgtgatca cacgccgttc ccccaccatg gagttgcgtg
      541 aacgtgcagc ctcaagtttt gtgattaatt cctgtgggag ataacgatcc aatttgaact
      601 gaggtttctc tcccccacct tccgtttcga cggcaagccg ggtgccgcaa ttcatgcaga
      661 attttgcagt ttcaggattt actgtccggc aatttgggca gttcataatc taccaatcat
      721 cctacaatgc tgaccgatcg agctccgcag tcgtgtgccc aattagggat cgcttcatcc
      781 ctggtatggc gtttggacga ttcacagtgt cccaaagcca tttaagatta taccgccgtg
      841 aattcgaaat gggaccaatc ctggcaacct gacgagcagg cccacccaac cgctatgaag
      901 tccggtggaa tagcggttgg gtgtcgaaac tcccgcgttt caggcgaggc tatgtctggt
      961 gatgttcagt tgttctgtgc cagagtgata accacttttc ccctggcatg tccttcattg
     1021 atatatcgga gagcatccgc gactccacgt aacgggtaac atctatcgat aactggcatc
     1081 actttaccgg cttcaataag ctctttcata aaaaccagat catcctggtt gggttttaca
     1141 agcatgttac ccattttttg gcttccggtc atcgaaatga ctggtccctg gagcatcgcc
     1201 tcgaacgttt gagccatggt acctccggtt ccgacataaa ttcccccggg acttaataca
     1261 cgcctgtact ctgaaagtga atgatacccg ttggcagcaa ggattaagtc ataacggtgt
     1321 ccattttgcg tgaaatcttc ccgggtgtaa tcaatgacgt gatctgcgcc aagcgagcgc
     1381 gccatgtcca aattcttcgt gctgcacacg gcagtgactt cagccccgaa cgatttggca
     1441 atctgcactg caaacgttcc cacaccaccc gatgccccat taatcaaaac cttttgccct
     1501 ggctgaatct ttcctttatc acggagaccc tgcagcgcaa cgactgagga ttcaggtaca
     1561 gcggctgctt cctcgaatgt caaatttgca ggcttcaacg cgattgcctt ttcaggaaca
     1621 gctacatatt cggaaaatgc accgaaaccg catacggaaa ggtcaccgaa cacctcgtca
     1681 cccggccgaa agcgttttac atttttgcca actgcttcca cctgcccagc catttccttg
     1741 ccgggtattt tgtgctttgg ttccaaaagt ccggtcgaga agcggaccac gaatggctcg
     1801 cctttcacga taatcacatc gccaatcgtt gcagcggctg cgtgaatttt taccagcact
     1861 tcatcctccc cgggggcagg tttttccact tctttgaact gaagaacatc tggtgatcca
     1921 tattttgtgt aaataatcgc tttcatttcg ttatcctttc tttatgatcc gttatgatta
     1981 atgatttatg gcaacttgtg agtttcgatt gcccggggta ccctgtactc tccagtacat
     2041 cgactgctct tcacgaattt gctggattcg tctgtggtac gagtgattct gcaggagtgc
     2101 tggtttttgc agacagggaa acgaatgcag atgaattgaa tcctttagcg atcagccaca
     2161 cacccatgaa tacttcccac aggccgccgg ggatcgagga aagatcaccc agaccggacc
     2221 ccatgattgc cgacaacatc ccgataaaaa tggttgcata tccaacgagt ccccagatgg
     2281 ccagccaccc cggcaccagc tttgccttgt acaaggtgta gcaaagcatc aacccggaca
     2341 ccccgagagc gctcattccg atctggtagg catattgttg tatctgggcg aacagagtgc
     2401 tcagggcttg aagagaggga gcgtcagagg ctgctgcttt caggtactca ctgcccagag
     2461 gtatctggat cattacaaag agaaccatga tggcgacgaa ggtggcgtcg atgatccgga
     2521 aagcgagata gccggcggcc atgcgctcac tgtgttgctt cagcaccggg aacatcagga
     2581 caccgtgagc ggcgtcgcca atgacggcca tcaaccagag taccgcgccg aagcccagca
     2641 gcgtactttt ggtggaaact gtagaaaggt agccgggccc actgagaatg gatgtgaaga
     2701 gtcccattcc cacaagacca actacgaacc ccaggaggta cacaaccccc acaactattg
     2761 cggtcgttcg gtatttattc acttctgttt ttgtattcat cgtattatct cctttgcgtt
     2821 gta
//
LOCUS       DASTJP010000883         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_87248, whole genome shotgun sequence.
ACCESSION   DASTJP010000883 DASTJP010000000
VERSION     DASTJP010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87248"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFQ23_25635"
     CDS             <1..262
                     /locus_tag="VFQ23_25635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910055.1"
                     /translation="AVFVFFLGVLIISYSIVIYVLVQDTRFNVLLASIFHLAINLTNL
                     LFLDVIYDLTFMIINSLVWAVVATGLVISNRELFLASIGKLT"
     gene            457..852
                     /locus_tag="VFQ23_25640"
     CDS             457..852
                     /locus_tag="VFQ23_25640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9910056.1"
                     /translation="MNETHTDVKPILIVENLPANLELFSLQLRQLGHSARHAPNGKVA
                     LDMYQANPHAFSMILMDLQMPVMDGLTATILIRQHEVNLGTHITIIALTANYAADIQE
                     QCLRAGMDDFVHKPITLDKLSQLLSKWLK"
     gene            1082..1336
                     /locus_tag="VFQ23_25645"
                     /pseudo
     CDS             1082..1336
                     /locus_tag="VFQ23_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
     gene            1709..2353
                     /locus_tag="VFQ23_25650"
     CDS             1709..2353
                     /locus_tag="VFQ23_25650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910057.1"
                     /translation="MIFDEMVYVGIEIASGSRPVIYACIDQEMNILLLENFSLPKVIT
                     HMRQNENIMLAVNASFRNNLASNENETLVFEILEKEITRAGFKPYLSNYACRQWVETY
                     PLDCFDSLSEQKPLPRETTTGQVQRAEILHAQGLRLNAARNFFAQDQDSVVNRTGKAF
                     YTPAELDALMAASIAWMLINRPVNIDLTRAPDQQMISIPREEKARWRKKPVFLR"
     gene            complement(2345..>2629)
                     /locus_tag="VFQ23_25655"
     CDS             complement(2345..>2629)
                     /locus_tag="VFQ23_25655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9910058.1"
                     /translation="DCAKRLHPDVLVMDVHMPVLDGFAAVKKIQDVAESTCVILMSLD
                     TDRVTAKQAAAVGAKGFLPKDDAARQLLSAIETVSRGETFFVEKTVSTLP"
BASE COUNT          702 a          620 c          622 g          685 t
ORIGIN      
        1 tgcggtcttc gttttctttc tgggggtgtt gatcatttcc tactcgatcg tgatttatgt
       61 gctggtgcag gacacccgtt ttaatgtgct gctagccagc atctttcacc tcgcgatcaa
      121 cctgaccaat ctgctgtttc tggatgtgat ttatgatctc actttcatga tcatcaacag
      181 ccttgtttgg gcggttgtgg caacaggtct tgtcatttcg aacagagagc tgtttctcgc
      241 atcaataggc aaactcactt gatgtagaag tcatcaaact gttaactgtg tattgagtcc
      301 aggctggcat aagtgaatta ccaaaagacc aaagccagtg cagcgttggt ctgcgagtac
      361 atgttgtata atcgttttgc ataacataca atgtcttaag aagcactgac acaacggaac
      421 attattcagg gcaggcggaa cgacgagata gaaaatatga atgagacaca tacagatgta
      481 aaacctattc tgatcgtgga aaaccttccg gcgaatctcg agttgttctc tttgcagctc
      541 cgccagttgg gacattcagc acgacatgct ccaaacggca aagtcgccct ggacatgtac
      601 caggcaaatc cacacgcatt ttccatgatc ctcatggatc tccagatgcc cgtgatggat
      661 gggttgacgg ccacaatctt gatccgccag catgaagtta atttgggaac ccacatcacg
      721 atcattgcgc tgacggccaa ttacgcggca gatattcagg aacaatgcct gcgcgccgga
      781 atggatgatt tcgtccataa gccgatcacg ctggacaaac tgagtcagct tctttcaaaa
      841 tggctgaaat agtgtagagc atgtaagttc ggtgaggaac actccgagac aaaattgttt
      901 ccaatctctt atcgcctgcg cagtaggaga gggggaaaac cctaattgac cgggttctca
      961 cttccgttca taatttcggc acaatctata aactttgcgg catgtgaaca gaaattcacc
     1021 attctgtagt gctaaattcg caaagtccaa atttgcaaga aatgtttgga ggaggcatac
     1081 tatggaaatg attaaagttt ctgcgaactc acgtacagct gcggtggccg gcgctattgc
     1141 gggagtggta cgtgaacatc agcgagctgt cgtgcaggca atcggtgcag gagcggtcaa
     1201 ccaggccgta aaagcactgg ttttggcaac cggttatctc aagaatgatg gtattcttgt
     1261 gtcctgcgtg cctgaatttc aagaggtaac aatcgacgat aaagtccgga ctgcaatcaa
     1321 attggtgatt gagccggagc cggatacaga tttttcaaat attcgaactg tcactcgatc
     1381 tctttcatca ccgagggcct aagtttgaat ccagaaccaa aaatctaata tacacatcaa
     1441 actcaggtag aaagccgtca attcatcaga tgagaacggc aactacctga gtgttttgtt
     1501 taagttcatg tcactaaatt tttagcatgg atagggagtt ccctacttgt ggccgtatcg
     1561 cccgaatgct attatccatg acggacgagg tgtctcctat cggttcacac gtccatctga
     1621 aggcggtgct gtactttagt gccgccttct atataaaatg gatttcactt agatttcaag
     1681 ggttttccag caggagaagg ttatcacgat gatatttgac gagatggtct acgtagggat
     1741 tgaaattgcc tctggttcac gcccggtcat ttatgcctgc atcgaccagg agatgaacat
     1801 cctgctactt gaaaatttta gccttccaaa agtaatcaca cacatgcggc agaatgagaa
     1861 cataatgctg gccgtgaatg catcgtttcg gaacaatctg gcctccaacg aaaatgaaac
     1921 cctggttttc gagatcctgg aaaaggaaat tacgcgcgcc ggtttcaagc cttacttgtc
     1981 aaattacgct tgtcgacagt gggtcgaaac ctatcccctc gattgcttcg actccctttc
     2041 ggagcagaag ccgctgcctc gcgaaacgac gacagggcag gtccagcgtg ctgagatttt
     2101 gcatgcacaa ggtctgcgcc tcaatgcagc gaggaatttt tttgcacaag atcaagactc
     2161 tgttgtcaac cggacgggaa aagcatttta cacccctgca gaattggatg cgctaatggc
     2221 ggcaagtatc gcatggatgt tgatcaaccg cccagtgaat attgatctga cacgtgcacc
     2281 agatcaacaa atgatttcaa ttccgaggga ggagaaagct cggtggagaa agaagcctgt
     2341 cttcctacgg tagggtactg acagtttttt ctacaaagaa tgtttccccg cggctcacag
     2401 tctcaatcgc cgaaagtaat tgcctggcgg catcgtcttt tggcaaaaag cccttggcac
     2461 ccactgcagc tgcctgcttc gcagttacac gatccgtatc cagagacatc aaaatcacgc
     2521 acgtagattc tgctacgtcc tgtattttct tcacggctgc gaagccgtct aacacgggca
     2581 tatgcacatc catcaccagc acatccggat ggagtcgttt cgcacaatc
//
LOCUS       DASTJP010000884         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1354970, whole genome shotgun sequence.
ACCESSION   DASTJP010000884 DASTJP010000000
VERSION     DASTJP010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354970"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="VFQ23_25660"
     CDS             complement(<1..1312)
                     /locus_tag="VFQ23_25660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910059.1"
                     /translation="MLITQPSRLTFWRLAFALSVALPLLSILQFIAAAPSYGVDISAS
                     RAWMNLLVGLGLIGIPPLLLLTLTWSRYQEHILHLAEFPERLPAGTRWIGVLFFVASM
                     VGFTLATKIPFIIFLTSFGWFRFLIFWCFTLLGILALKSVWRDVPWLTALLVTVLCQA
                     VFHLLLFYWTKVTDYPFAMGWSETSRFYHPSLFLSEGVYGERFAWPILHPTLHLLLAP
                     PYLFDAPLWVHRFWQVALMYMLLAAVVPALMKRLSIEGTFTRWLVGIWMFLFLFMGPV
                     YFHLTIPVILVLLGFSTENDRRTWIVVIVASIWCGWSRVNWYPMPGMIAAVLYLMEVP
                     IRGKSIWRYLLKPALWFVVGILIAFLSQRIYIALSGVPDSELFYTSFTSNLLWYRLMP
                     NASYFLGILPAAILASIPIWLAMYLVFRSRRTDWHPVRLSLILAA"
     gene            complement(1352..2527)
                     /locus_tag="VFQ23_25665"
     CDS             complement(1352..2527)
                     /locus_tag="VFQ23_25665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9910060.1"
                     /translation="MSILDQEKRQFSILLIGTQMTIGGAQRGLFDQARWFKSQGCKVA
                     VVFFYDKEGFHENWSQAVDFPVYNLQAYQRGAGPLRQGRLLLRGLWRLWGLLKREHFD
                     VLETFTHDSNTLGLPLAWLARVPVRIATHRGKIEAFPKWRQVLHNLLINSPITHTLIA
                     VSEQTRGQAIEEGVRPSKITVIPNAVKQLDTSTVDRDAVRKELGVHENDIFLLSIGRL
                     TYQKGHEFLIQAMSKVVSRFPNVKVGICGEGPLRSQLEKQIRDMGLSDHVRLLGAWDD
                     VAPLVASADIFVLPSRWEGLSRALMEAMAAGLPSIAAQVDGIKDLITDDVNGLLIPAE
                     DSEALENSILQLIKDAEMRKRIGAAGQAHVLTVHSVDDMCLKYYRVMLGFLEAVNHA"
     gene            complement(2534..3643)
                     /locus_tag="VFQ23_25670"
     CDS             complement(2534..3643)
                     /locus_tag="VFQ23_25670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9910061.1"
                     /translation="MQRENSSLPRIGIFFHDLGIGGAERVMLQLARGFIEAGHPVDLV
                     LARAEGPLAAEVPAGVQIINLETRRPLQMFFGLVRYLRRKKPSVLLSPFEVTSVIAIL
                     AKTLTRVPTRIVIRVSVHISRNKRNATWKKTLERLVVSWLYPLADGIITVSQGVAKDL
                     AVYTGIPLARIKVIYNPIISNRLHEAAAEPIHHPFFMDGQEPVILGAGRLAEQKDFST
                     LIRAFDLLRKKIPARLIILGDGEERAHLEELIRSFGLQGMVDLPGFNTNPFAFMKQSS
                     VFVLSSRWEGLPGVLIQAQACGCPVVSTDCLSGPSEILKGGEYGHLVPVGDVQEMAKA
                     MEATLLGDVRKPPQSWLAQFTLQVVIPQYKAVFGI"
     gene            complement(3627..>3871)
                     /locus_tag="VFQ23_25675"
     CDS             complement(3627..>3871)
                     /locus_tag="VFQ23_25675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910062.1"
                     /translation="KFPLDARFLVCGRLTDNERLKGYSFYYLHGEPESVTQLRVSQEY
                     PDATRLFRSIYGVITGFILILLIVGFIRDINRATRK"
BASE COUNT         1126 a         1003 c          901 g          841 t
ORIGIN      
        1 acgcagccag gatcaagctc aaacgaacgg gatgccaatc cgttctgcga gaacgaaaaa
       61 caaggtacat tgcgagccaa atcggaattg atgcaagaat agccgccggg aggatgccca
      121 gaaaataaga tgcgttcggc atcaacctgt accacaataa attagatgta aagcttgtat
      181 aaaaaagctc gctatcgggc acgccagata aggcaatgta gatgcgctga gacagaaatg
      241 cgatcaatat ccccaccaca aaccaaagag caggtttgag tagatatcgc cagatacttt
      301 ttccacggat aggaacttcc ataagataaa gcacagccgc gatcatccca ggcatgggat
      361 accagttcac acggctccag ccgcaccaga tggatgcaac gataacgaca atccaggttc
      421 gcctatcgtt ttcagtggag aaacccaata agaccaggat cacagggatt gtcagatgaa
      481 aataaacggg tcccatgaac aggaaaagaa acatccatat cccaaccagc cagcgggtga
      541 aagttccctc aatagaaagc cgcttcatca acgcagggac aacggccgca agcaacatat
      601 acatgagcgc cacctgccag aatcgatgaa cccaaagcgg cgcatcgaaa agataagggg
      661 gcgcaaggag taaatgcagc gtaggatgca gaataggcca ggcaaatctc tcaccgtata
      721 cgccttcaga gagaaacaga gaaggatgat agaagcggct ggtttctgac cagcccattg
      781 caaacggata atccgtcacc ttggtccaat aaaataacaa cagatgaaat accgcctgac
      841 aaagtacagt cacaagcaat gctgttagcc acggaacgtc gcgccatacg gatttcagtg
      901 ccaaaatacc cagcagggta aagcaccaga agatcaggaa ccggaaccag ccaaaactcg
      961 tcagaaagat gataaatgga atcttcgtgg ccagggtaaa tcccaccatg gaggcaacaa
     1021 agaacaaaac tccgatccag cgcgtcccag ccgggaggcg ctcagggaat tccgccagat
     1081 ggaggatgtg ttcctgataa cgggaccagg tcaacgtcag aagaagaagc ggtggaatcc
     1141 caatcaagcc cagcccaacc agcaaattca tccatgccct gctggcagag atatcaaccc
     1201 catagctggg ggctgccgca atgaactgca gaatggaaag caaaggcagg gctacactaa
     1261 gcgcgaacgc aagccgccaa aatgtgagac gtgatggttg ggtgattaac attcgattct
     1321 aaaaaacagg agaggtttcg ctgtcagcta ttcaagcatg attaacggct tcaagaaaac
     1381 ccagcatgac ccggtaatat ttcaggcaca tatcatccac agaatggact gttagaacat
     1441 gcgcctggcc ggcggctccg attctctttc tcatctctgc atctttaatt aattgtaata
     1501 tggaattctc aagcgcttca ctatcttcgg cagggatcaa aaggccattc acgtcatctg
     1561 tgattaaatc cttgattccg tctacttgag ccgcaatcga cggcaacccc gctgccattg
     1621 cctccatgag agcccgcgac aatccctccc agcgcgaggg caagacaaaa atatctgcag
     1681 aagctacaag cggagcaaca tcatcccatg cgcccagaag acgcacatgg tcagacagcc
     1741 ccatgtctct aatttgtttc tccaattgtg aacgtaacgg accttcgcca catattccga
     1801 cctttacatt tggaaaacgg cttaccactt tagacatcgc ttgaatgaga aattcatgtc
     1861 ccttctgata agtcagccgg ccgatggaaa gaaggaagat atcattttcg tgcacaccca
     1921 gctcttttcg cactgcatct cgatcgactg tggaagtatc cagttgttta actgcattgg
     1981 gaatcacagt gattttacta ggtctaaccc cttcctcgat ggcttgtcca cgggtctgct
     2041 ctgagactgc aatgagcgta tgtgtgatcg gggaatttat caacagatta tgtaacacct
     2101 gacgccattt tggaaatgct tcgatctttc cgcgatgggt cgcgattcgc acggggacgc
     2161 gggcgagcca ggcaagaggc aatccgagtg tattactgtc gtgggtaaaa gtctccagga
     2221 catcaaaatg ttcacgcttc agcagccccc ataatcgcca taaacctctt aataataatc
     2281 tgccttgacg caagggacca gcccctcgct gataggcttg caggttgtac acaggaaaat
     2341 caacagcttg gctccagttt tcatgaaatc cttccttatc gtaaaagaag acgacggcca
     2401 ccttacagcc ttgtgattta aaccagcgcg cctgatcgaa caagcctcgt tgtgccccgc
     2461 cgatcgtcat ttgagtgcca atcagcagaa tggagaactg ccttttttct tgatctaaaa
     2521 ttgacatctt tgcttaaatt ccgaagacag ctttatattg cgggatcact acctggagtg
     2581 taaattgagc caaccagctt tggggcggct tgcgaacatc tcccagcagg gttgcttcca
     2641 tggcctttgc catttcctgc acgtccccga ccgggacgag atgcccatat tctccaccct
     2701 tgagaatctc agatggaccg ctcaggcaat cggtgcttac caccgggcag ccacaagcct
     2761 gggcctggat caacaccccc ggcaggcctt cccacctgga agataagaca aacacggaag
     2821 actgcttcat gaaggcaaaa ggattggtat tgaagccggg aagatcaacc atgccctgta
     2881 gcccgaatga gcgaattaat tcttcaaggt gagccctttc ctccccatca ccgagaataa
     2941 tgagtcgtgc tggaatcttt ttgcgaagaa ggtcgaacgc acgtatcaat gtggaaaaat
     3001 ctttttgctc cgccaagcgt ccggcgccta gaatcacagg ctcctggcca tccataaaga
     3061 aaggatgatg aatcggctct gcggctgctt catggagtcg attcgagata atcggattgt
     3121 aaatgacttt tatacgcgct aaaggtatcc cagtatatac agcaagatct ttcgccacgc
     3181 cttgtgaaac cgtgataatc ccatctgcaa gaggatataa ccacgaaacc accaaccgct
     3241 cgagggtttt cttccatgtt gcattacgct tgtttcgcga aatatgcacc gacacccgaa
     3301 tgacgattct ggttggcacg cgtgtcaatg ttttcgccag gatggcgatc acactggtta
     3361 cctcaaaggg cgatagcaac acactcggtt tttttctgcg tagatatctg accagcccga
     3421 aaaacatttg tagaggtctt ctggtttcaa ggttaatgat ttgaacaccg gcgggcactt
     3481 cagcagccaa cggaccttcg gcgcgcgcca aaaccaaatc cacagggtgg cccgcttcga
     3541 taaatccccg tgcgagttga agcatcacgc gttccgctcc gccgatgcca aggtcgtgaa
     3601 agaatatccc aattcgagga aggctgctat tttcgcgttg cacgatttat atctctgatg
     3661 aatccaacaa taagcaggat aagtatgaat ccagtgatca ccccgtagat agatcgaaac
     3721 aaacgagttg catcaggata ttcctgtgaa actctcagct gagttacaga ttcaggttct
     3781 ccatgcaaat agtagaaact atagccctta agccgttcgt tatcggtcaa gcggccacag
     3841 accaaaaatc tagcatccaa cggaaacttt g
//
LOCUS       DASTJP010000885        12166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_132689, whole genome shotgun sequence.
ACCESSION   DASTJP010000885 DASTJP010000000
VERSION     DASTJP010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12166
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132689"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..684
                     /gene="rdgB"
                     /locus_tag="VFQ23_25680"
     CDS             46..684
                     /gene="rdgB"
                     /locus_tag="VFQ23_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET9910063.1"
                     /translation="MKQLLIATNNKGKIKELQDLLLDTGIELITPAQIQLELDVIEDG
                     KTYAENATKKAIAFAAASQLVSLADDSGLEVDALDGAPGLYSARYGPPLNLPPFAEKI
                     GGGRRGLTDADRRAYLLENLRDKPRPWIARFHATIAIANSNKEIHLTDGICEGEIIPE
                     ERGSGGFGYDPIFLLTDLGKTMAELSIEEKNRLSHRARAVIKAKSILERIFK"
     gene            complement(688..2448)
                     /locus_tag="VFQ23_25685"
     CDS             complement(688..2448)
                     /locus_tag="VFQ23_25685"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9910064.1"
                     /translation="MRRFESPYLADWFATSLRWVVLVGLIISLSLRGEIITEHNIWSL
                     GLLFIWNVIMSLLAGLSVRIIQYHRQIVLSVDFLLAAIFFWLHGGLDGSSAWVGLYPI
                     LTSAVYFEMWGAFITATLFALLQYVVSPDPFSNGFTLITFSGLALTLLIGMASGLTGR
                     FLMRRLRTERHHRLDEEQRRQRMESERLRAIYELTSTLTATLNYKRVLDSALDLAYTV
                     LDPNPDPEEPNRDERLVSAVFLFKGNELQVGSARRFTNADLRASLRGNEGVLKKIFDE
                     GDAILTHDIGYDPELGRIVALRSCTSAYCFPLRSGFNVYGAMLFAHPDPIYFTPERCG
                     LLDIIGRQAVIAVQNASLYQNLVQEKERMVEVQEEARKKLARDLHDGPTQSVAAMAMR
                     INLARRMMAKDAEAGVDELVKIEELAHRTTKEIRHMLFTLRPLILESQGLTAALEAMA
                     EKMRETFGQNVLINVDEVVLENLEMGKQGVIFYIIEEAANNARKHANALHIWVRLRSF
                     ESGIALLEIEDDGLGFDLEAVNKSYDKRGSLGMVNLRERAELVHGLLNIDSAPGKGTR
                     VQVYIPLTEEAADRLHHAKM"
     gene            complement(2497..3225)
                     /locus_tag="VFQ23_25690"
     CDS             complement(2497..3225)
                     /locus_tag="VFQ23_25690"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9910065.1"
                     /translation="MIILSVGMPRAGSGWHYNLVHDLMKTAGATDASDIRQRYRLQSI
                     LTEINNNIGVLSARRLVMVTLPALMANTFVIKAHAGPTSASRILQSLGLLRITYIYRD
                     PRDAMLSAFEYGQRGLQRGRPNAFSHLTDFQKSLDFIMDYVRIWDRWTREKNIFITRY
                     EDLQTNYQQETARLVKFLKLNEDKPEVQKVIEEYHQPVVEGQKGLHFNKGKIGRFREA
                     YTAQEQAILQEKMDSYLPRMGYQI"
     gene            complement(3222..4670)
                     /locus_tag="VFQ23_25695"
     CDS             complement(3222..4670)
                     /locus_tag="VFQ23_25695"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9910066.1"
                     /translation="MNRLTRFLWGAALLTLPVTSFRYFPFLGDATYVRPLAFYPIAFL
                     LPLLLIQLWRGKTSMPRAGAVIPLIVFVMFTLAATGFGALLDPLPLRGQEYAGRAIRA
                     WVTLVIGLCFFIAAVWMNRDQEDLKFTVTWLMAGFAMDVVWSGIQSLAFYTPLLEKVT
                     VTHWQRAFSMRELVRTNRVSGMAYEPAWLAGQIVTVYLPVFFASLLTGIRMTRSKWLE
                     VILLGFGFLLLLATFSRGGLLIGVVASLLTFLVAGRSELRGAWNWFLSGFRREGGWIL
                     RIVVVLIFFGALAGAGLFLSQKGYITRIFNTQAESLEDFIIKNSAGARAAYTFGALGA
                     YDKNPISGVGLGASGLYIYANLPNWAMTTVPEIARQLSPENRLYPTPKNMYARLLAET
                     GLFGFILYFSFLFSVLGDSLLGLQRNNLFMRYLGIAGFFSLIAIGLYNNTQDSFATPN
                     IWLIPGVVTGIMSNHNFIAHSKDAVGSGQENQ"
     gene            complement(4667..5335)
                     /locus_tag="VFQ23_25700"
     CDS             complement(4667..5335)
                     /locus_tag="VFQ23_25700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910067.1"
                     /translation="MIRADMNRLFSSPSLDDLIRLLKAWRYWTIGGIVGALIGAAFFY
                     LAPPAYQARATVLVDFNLEQAWPQETDRQQFFYLERETRKLEDVAWSDQVLNTLSSDF
                     SIPVNELRDGKLRLSQPAEAGWHFYAEDKNPEIAQELAGSWAQAFAAQVTEDVANATD
                     LNSFIEVEVTQAENLPVERSTALSEYLFAGAIGFLLLSSFFILFFSPAILRSPSGSGA
                     GGEG"
     gene            complement(5322..6107)
                     /locus_tag="VFQ23_25705"
     CDS             complement(5322..6107)
                     /locus_tag="VFQ23_25705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9910068.1"
                     /translation="MTDQPLVTILTPSFNQARFLEATIKSVLSQDYPRIEYMIVDGGS
                     SDGSVDLIKTYENQLAWWVSEKDKGQTDAINKGFARANGEFLAWINSDDTYEPGAVSA
                     AVKYLQEHPEIGMVYGDCNFINEEGRVIGRFGSAQTNYRLLRQGYAHIPQQTMFFRAD
                     LWNQVGPLDPSFYFAMDYDLWTRIAARTQIKYVPQTWANFRLHTSGKTMAADDRCWPE
                     MIRVHYRDGGSFFSVIVAKYYIRKLIAPLWNWRRQRMLRVDPR"
     gene            complement(6104..7033)
                     /locus_tag="VFQ23_25710"
     CDS             complement(6104..7033)
                     /locus_tag="VFQ23_25710"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9910069.1"
                     /translation="MEEHKLQPLLLTGAHRSGTTWIGRMLALDPSTAYISEPLNVLHR
                     PGVFRVPIHHWYQYICEENEGEYLPAFHEVLEFKYHFWDEIRSIRSLRDFLRMGRDFK
                     IFYSGLEEGRRALLKDPFAVFSTEWFAQKLNVKIVISVRNPLGFVSSLKRLNWTFDFQ
                     DLLDQPLLMRDHLEAYRSQMESAQMKNVIEQGALLWKMIYKTVHSTLSRNPDFIIVRH
                     EDFSRDPITEFRKLYQTLGLDYTHHIDKTILDSSSSENPMELSGKKVHDVRLDSRANM
                     SNWKKRLTEDEINRVRAVTEDVAQLFYTDAEWA"
     gene            complement(7040..8491)
                     /locus_tag="VFQ23_25715"
     CDS             complement(7040..8491)
                     /locus_tag="VFQ23_25715"
                     /inference="COORDINATES: protein motif:HMM:NF039639.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HET9910070.1"
                     /translation="MRQKLSEEQWLWLALFLGIVTTIIGLGIFAYLGIFSRYTSDDYC
                     LSAFFLNDNLIDEMTRRYYVSSSRYTNILFIGLVDLLFGWYNVAILPVLMLALFVLGL
                     YFFLKEIVRMCRLRWDHWMIFFLAASVAFFSVLQAPALYETLYWRAGMTSHFAPLVFI
                     PFFGAFLLRQISLAEKRFPPLWIQLVCLISPLIIGGFSEPPTTVMITTLLLAIIWTWI
                     WGNKNVRKPALILLAWSFVGAVLALIVLALAPANSLRLDRPPPGLFELINRIIIYPFD
                     FIVDTLRTRPTPTIISVMMPAILFYVYYSQPARSISNEERRRLGMLTIVMLLLAYLLI
                     AASFAPSAYGQSYPVGRARFAGRLIMTIALLAEGALLGVLLASIKTNLSQNIYVRSFG
                     MLALMVIMFYPLRIAWQTSMQIPAYQQRAAAWDERDSEIRALSSQGVRELVVPFLSGD
                     PIQDLGDRREFRLNRCAAALYGVDSILAVPMEE"
     gene            complement(8511..9254)
                     /locus_tag="VFQ23_25720"
     CDS             complement(8511..9254)
                     /locus_tag="VFQ23_25720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9910071.1"
                     /translation="MSFDYDSVAGDYQYRALHSGHPMQRFWHGGKLTMIDRLIRPHLK
                     ADSKLLEVGCGAGNLLLQATVRGSYPVALDLAKQALTFVRSRLREAKSGSEAPRDFMC
                     IQSIGELLPLASDSFECILLSEVIEHLEQPQISIKEAARVLRPGGRLLVTTPNYRSFW
                     PLMERAVDMLNMAPKMAGEQHISRFHSKSLKNLLIESGLKIDYLGTIFFLSPFLSLIS
                     SKWAERQLERELNRKSAIGMILVAVAVKP"
     gene            complement(9265..9996)
                     /locus_tag="VFQ23_25725"
     CDS             complement(9265..9996)
                     /locus_tag="VFQ23_25725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9910072.1"
                     /translation="MTALDLSIIIPAYNEEALIVNTLESLRVYMSARTEQYEVIVVDD
                     GSQDKTVLCVQEWQKRSGINLRLLSNQQNMGKGFSIRHGVMESRGQHIIFIDADLPYE
                     LDAIANFLSALKSGFDLAVGSRVLPGSEVRGVPIYRYIAGQIFSWMVQAVLFSGLPDT
                     QCGFKSFKSEAAREIFRRTSINGFGFDVEMLFVARKLKLTIQPVPVHMIEHRQRSRVR
                     LVNDSLKMFANLFMVRWMDLQGKYE"
     gene            complement(9993..11570)
                     /locus_tag="VFQ23_25730"
     CDS             complement(9993..11570)
                     /locus_tag="VFQ23_25730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9910073.1"
                     /translation="MSQPSFFSSPVSRGIALVLLFSLALTIRLYDLTDLPLDFHPTRQ
                     LLSAIKARALYYETQPDGISTQRLETAIRQAKLKADVEPVIFERLVAFTYRFTGEQVW
                     IARIYSSLFWLAGGLFLFLLVRDLSSFDGAIFATAYYLFFPYSVLASRSFQPDPLMVM
                     FILAFWWAFSRWFDLSSIGAERSVSEDEAARGKGWRWALLAGLLGGLAILIKFSAAFF
                     VIGPALGLALSRFTLRELLKHPHVWLMAVIGILPAAIYLIYGIFIGGYLGAQFSGRFI
                     PELLLSPVNYVLWGSKATLAAGGLFIMLGLLGLLLVKEVRLRSLMRGLWIAYLFYGLF
                     FNYHVATHDYYHLVLIPIVAVSLSPLGDWFLGRLAESSPRPWMRTAASFILILGLSSV
                     LWDIRNQLKAVDYRPEAAMWAEVGEGLADERVVALTQDYGSRLEYWGLKTAATWPYVG
                     DINYIDTRGGSFSFDELFEKYSSQRDFFLVTDFDELDRQLDLKNRLFSSYLIHAQGDG
                     YVIFNLRSPLGGGYSGS"
     gene            complement(11602..>12166)
                     /locus_tag="VFQ23_25735"
     CDS             complement(11602..>12166)
                     /locus_tag="VFQ23_25735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910074.1"
                     /translation="QTINNLKYKQLSLEDLLCFQIIGQPAVFFRRAAFEKAGRLDTGF
                     HFLLDHHLWIRLAQQGRILHVPQTSAAARYHAEAKNRAKAAEFGREAFRILDWAKSQS
                     KLAGVISPVERRARASAHRVDARYRLDGGQPWSAVKAWMRAFFIHPPTALARLNILVA
                     SLLNLTGLNALREFILRRRQRGYSSDK"
BASE COUNT         3448 a         3151 c         2852 g         2715 t
ORIGIN      
        1 cacggtggat attacacagt ggactttgga agcccctaac tcaatatgaa gcaacttctc
       61 atcgccacaa acaacaaggg aaaaataaag gaactgcaag acctgttgtt agatacaggc
      121 attgaactta ttacacccgc ccagatccaa ctcgaactgg atgttatcga ggatggcaaa
      181 acatacgctg agaatgccac taaaaaagcc atcgccttcg cagcagcaag ccagctcgtt
      241 tcacttgccg atgactcggg cttggaagtg gacgcactgg atggcgcgcc agggttatat
      301 tcagcaagat acggcccccc tcttaatctc cccccatttg ctgagaaaat agggggaggc
      361 agaagggggc tgactgatgc agaccgccgt gcctatttat tagagaatct tcgagacaaa
      421 ccccgtccct ggatcgcgcg cttccacgct acgattgcca ttgcaaattc caataaagaa
      481 atccacctca ctgacggaat ttgtgaaggt gagatcatcc cagaagaacg cggcagtggc
      541 gggtttgggt atgatcctat atttcttttg actgacctgg ggaaaacgat ggcagaactt
      601 tcgatcgaag aaaaaaatcg cctcagccat cgggcaaggg cggtgataaa agcaaagtca
      661 attttggaac gaatcttcaa ataagtatta catcttggcg tgatgcagtc gatctgctgc
      721 ttcctcggtg agcgggatat agacctgtac gcgcgtccct ttccccggtg cggagtcaat
      781 gtttagcaac ccgtgtacca actcagcgcg ttcgcgcaga ttgaccatcc ccaagctccc
      841 acgcttatcg taggacttgt tcacggcctc caaatcgaat cctaacccgt cgtcttcgat
      901 ctccaataag gcaataccgg attcaaagga tctgagtcgc acccagatgt gcagggcatt
      961 ggcatgcttt cgtgcattat tcgccgcttc ctcaataata tagaagatca cgccttgctt
     1021 gcccatttcc aggttctcaa ggacgacctc atccacattg atcagcacat tctgaccgaa
     1081 ggtctcacgc attttttctg ccatcgcttc aagcgcggca gtcagccctt gcgactccaa
     1141 gatgagtggg cgcagagtaa acagcatatg ccggatctct tttgtcgtcc tgtgagccag
     1201 ttcctcgatc ttgactagct catccactcc ggcctctgca tcttttgcca tcatgcgacg
     1261 agcaaggttg atgcgcattg ccatcgcagc gaccgactgt gtaggaccat catgcaggtc
     1321 acgtgccagc ttcttgcgcg cttcctcctg cacttccacc atccgttctt tttcctgtac
     1381 caggttttga tacaggcttg cattttgaac cgcgatgacg gcctgccggc cgataatatc
     1441 gagtaatccg caacgttctg gcgtgaaata aattgggtct ggatgagcaa ataacatcgc
     1501 gccataaacg ttgaagccac tgcgcaaagg gaaacagtaa gcagacgtgc atgagcgcaa
     1561 cgcaacgata cgtccaagct cggggtcata cccgatgtca tgcgtaagga tcgcatcccc
     1621 ttcatcgaat atctttttga gaacaccttc gttaccacgt aaactggctc gtaagtctgc
     1681 atttgtgaaa cgccgtgcag agcccacctg caattcatta cccttgaaca aaaatacggc
     1741 actgaccaat cgttcgtctc ggtttggttc ctcaggatca gggttcgggt cgagaacggt
     1801 atatgccagg tcaagtgcag agtcgagtac gcgtttgtaa ttgagggtcg ctgtcagagt
     1861 ggaggtgagt tcgtagatgg cgcgcaggcg ttcgctttcc atgcgctggc ggcgttgttc
     1921 ctcatccagg cgatgatgtc gctcggtccg cagtcgtctc atcaagaacc gtcccgtgag
     1981 accactcgcc atgccgatca gtaaagtcaa tgccagtcca ctgaatgtaa tcagggtaaa
     2041 tccatttgaa aacgggtcag gagaaacaac atattgcaac aatgcaaata aagttgctgt
     2101 gatgaacgca ccccacattt caaaatatac cgcacttgtg agaatgggat aaagcccaac
     2161 ccacgcagag gaaccatcca gaccaccgtg cagccagaaa aatatcgctg ccagcaagaa
     2221 atccacactc aacacgatct ggcggtggta ttgaataata cgcacgctca gacccgccaa
     2281 caggctcata ataacattcc agataaataa gaggcccaac gaccaaatat tatgctccgt
     2341 gatgatttcc ccacgtaacg agagagagat gatcaaccca accagtacca cccaccttaa
     2401 cgaagtggca aaccagtcag caagataagg agactcgaac ctgcgcatac ggtcaatgat
     2461 actcgaaaaa acaaaccctt cgctggagcc tcagaattag atttgatacc ccatacgcgg
     2521 caaataggaa tccatttttt cctgcaggat cgcctgttcc tgagcagtat acgcctcacg
     2581 aaatcgaccg atcttgccct tattgaagtg cagacccttt tgcccttcga cgactggctg
     2641 atgatattct tcgatcactt tttggacttc tggcttgtcc tcgttcaatt ttaggaactt
     2701 taccaggcga gcagtctcct gctgataatt cgtctgcaaa tcttcgtaac gcgtgataaa
     2761 tatatttttc tctctggtcc atcgatccca gattcgtaca taatccataa taaaatcaag
     2821 gcttttttga aagtctgtca gatgagaaaa cgcattcggg cggcccctct gcagcccgcg
     2881 ctgtccgtac tcgaacgcgg aaagcatggc gtcgcgcggg tctcgataaa tataggtaat
     2941 gcgaagcaag cccaggctct gcaaaatacg ggaggctgaa gtgggacctg catgcgcctt
     3001 gatcacaaac gtgttggcca ttagggcagg cagtgtcacc atcaccaatc ggcgggcaga
     3061 cagcacgcca atgttgttgt tgatctccgt caggatgctt tgcaatcgat accgttggcg
     3121 aatatcgctc gcgtcggttg cacctgctgt tttcatgaga tcatggacga gattgtagtg
     3181 ccagcctgaa cctgcgcgag gcattcccac cgaaagtatg atcattgatt ctcctgcccg
     3241 ctcccaacgg catctttgga atgagcaata aagttatggt tggacatgat gcctgtcaca
     3301 accccaggta ttaaccagat attgggtgtg gcaaacgaat cctgagtgtt attatacagg
     3361 ccgattgcga tcaacgagaa aaagcctgca atacccagat aacgcatgaa gagattgttt
     3421 ctttgtagcc ccaaaagcga atcaccaagt acggaaaaca ggaacgaaaa atagaggatg
     3481 aaaccaaaaa ggccggtttc tgcaagtaag cgtgcgtaca tatttttagg agtcgggtat
     3541 aaacgatttt ccgggctgag ttgacgggcg atctctggaa ccgttgtcat tgcccaattc
     3601 ggcaggttgg cataaatata caatccgctt gcgcccagtc ctacgccact gattgggttt
     3661 ttatcataag cacccaatgc accgaatgta taggcagccc gggcgccagc tgaatttttg
     3721 ataataaaat cttcaaggct ttcagcctgt gtgttgaaga tacgcgtgat ataacctttt
     3781 tgactcaaaa ataggcccgc acccgctaac gcaccaaaaa agatcaatac aacaacaatg
     3841 cgcagaatcc aaccgccttc tcggcgaaag cctgaaagga accaattcca cgctccacgc
     3901 agttctgatc gccctgcaac aagaaatgtc agaagcgatg caaccacacc aatgagcagc
     3961 ccgccccgtg aaaacgtggc aagcaataac agaaacccaa agcctaatag aatgacctca
     4021 agccatttag accgcgtcat tcggattccg gtcaagagtg aggcgaagaa aactggcaga
     4081 tacacagtaa caatttgccc tgcgagccag gcaggctcat acgccatgcc agaaacccga
     4141 ttggttcgca cgagttcacg cattgagaag gcccgttgcc agtgcgtgac agtaactttc
     4201 tcgagcagcg gcgtgtaaaa tgccagagac tgaatgccac tccacaccac atccatcgca
     4261 aacccagcca ttagccatgt aacagtaaac tttaagtctt cctgatctcg attcatccat
     4321 acggctgcaa taaaaaagca taaaccaata accagcgtga cccatgcacg gattgcacga
     4381 cccgcgtatt cctgtccgcg caacgggagc gggtcaagca gtgccccaaa accggttgcc
     4441 gctaacgtaa acatcacaaa cactatgagt gggatcactg cgccggcgcg cggcatcgat
     4501 gttttgccgc gccacaactg gatgagcaag agtggaagaa gaaaagcaat cgggtaaaac
     4561 gcgagtgggc gaacgtatgt cgcgtcaccc agaaaaggaa aataacgaaa actggtaacc
     4621 ggtaatgtaa gcaacgccgc gccccacagg aagcgcgtaa gtcgattcaa ccctcacccc
     4681 cggccccact tccactggga gagcggagaa tagcggggga aaaaaacaaa atgaagaaag
     4741 aacttaagag tagaaacccg attgcccctg caaataaata ctcgctcagt gcagtgcttc
     4801 tctcaaccgg aaggttttca gcttgcgtca cctcgacctc aatgaatgag ttgagatccg
     4861 ttgcgttcgc tacatcttca gtgacttgtg ccgcaaatgc ctgcgcccag gagccagcaa
     4921 gttcctgcgc aatttcaggg tttttgtcct ctgcatagaa atgccaccct gcttctgctg
     4981 gctgactcag tcttaatttc ccatcacgga gctcattgac cggaatcgaa aaatcagaag
     5041 atagcgtgtt caatacctga tcagaccatg caacgtcttc gagcttgcgt gtctcgcgtt
     5101 ccaggtaaaa gaattgctga cgatcggttt cctggggcca ggcttgttcg aggttgaagt
     5161 ccaccaggac ggttgctcgt gcctgataag caggtggcgc aagataaaaa aatgcagcgc
     5221 cgatcaacgc accgacaatg ccgccaatcg tccaataccg ccaggctttc agcagacgga
     5281 tgagatcatc gagagaagga gatgaaaaca accggttcat atcagcgcgg atcaaccctc
     5341 aacatccttt gccgtcgcca attccagagc ggtgcgatca gtttgcggat gtaatattta
     5401 gccacgatga ccgaaaagaa cgagccaccg tcacgataat gaacacggat catctcgggc
     5461 cagcaacgat catccgcagc catagttttc cccgaagtat gcaaccggaa gtttgcccag
     5521 gtctgaggta catacttgat ttgcgtgcgt gcggcaatgc gcgtccacag atcataatcc
     5581 atggcaaaat aaaaagaagg atccagagga ccgacctgat tccataagtc cgcgcggaag
     5641 aacatagttt gctgagggat gtgagcataa ccctgccgca gcaaccgata attcgtttga
     5701 gcagacccaa acctgccaat cacacgaccc tcttcattaa tgaaattaca atccccatag
     5761 accatgccaa tttcggggtg ttcctgcaaa tacttaaccg ccgcactcac cgccccaggc
     5821 tcgtaggtgt catcggagtt gatccaggcc aggaattcac cattggctcg cgcaaatcct
     5881 ttgttgatgg catccgtttg acctttgtct ttttcgctca cccaccaggc aagctggttt
     5941 tcatatgttt tgatgaggtc aacgctccca tcactggaac caccgtccac gatcatgtat
     6001 tcgatccgtg gataatcctg tgatagcaca gacttaattg tcgcttcaag aaaacgcgcc
     6061 tgattgaatg acggcgtaag tatggtgaca agcggttgat ctgtcatgcc cactccgcat
     6121 cggtataaaa caattgagca acatcttcag tcactgcgcg gacccggttg atctcatcct
     6181 cggtgaggcg cttcttccaa tttgacatat ttgcacgact atcgagccgc acgtcatgaa
     6241 ctttcttacc ggataattcc atggggtttt cagagctact ggaatctaaa attgttttat
     6301 cgatgtgatg agtatagtcg agaccaagtg tctgatacag tttgcggaat tcagtaatgg
     6361 gatcccgcga gaaatcttcg tggcgaacga taataaaatc gggattccga ctaagcgtgg
     6421 aatggacagt cttgtaaatc atcttccaga gcaaggctcc ctgttcgatg acatttttca
     6481 tttgggcaga ttccatttgt gagcgatagg cttccaaatg atcgcgcatc agcaacggtt
     6541 gatcgagcaa atcctgaaag tcaaatgtcc aattcagacg tttgaggctg ctcacaaacc
     6601 ccagcggatt ccgcacggag atcactattt tgacgttcag cttctgtgca aaccattccg
     6661 tggaaaagac cgcgaatgga tccttgagga gcgcgcgccg tccttcctcg agcccggaat
     6721 agaaaatctt gaaatcccgt cccatgcgca ggaaatctct gagagaacgg atggagcgaa
     6781 tctcgtccca aaaatggtac ttaaattcca gcacttcatg aaaggccgga agatattcac
     6841 cctcattctc ctcacaaata tattgatacc aatgatgtat tgggacccga aaaacaccag
     6901 gacgatgcaa aacgtttagt ggttcactga tatatgcggt ggatgggtcc agtgcaagca
     6961 ttcgaccgat ccaggtagtc ccactccgat gcgcaccggt caacaggagg ggctgaagtt
     7021 tatgttcttc catcttacgt tattcttcca tgggaacagc caggattgaa tctactccat
     7081 aaagcgccgc agcacaacgg tttagcctga attccctgcg atctcccagg tcctgaatag
     7141 ggtcaccgga gaggaacggc acaaccagct cgcgaactcc ctgactgctg agggcgcgga
     7201 tttcagaatc acgttcatcc catgcagccg cgcgttgttg gtaggcagga atctgcatcg
     7261 aagtctgcca ggcgatccgc agaggataga acataataac catcaaagca agcatcccaa
     7321 aactgcgaac ataaatattt tgtgaaagat ttgtttttat acttgccaga agtacaccca
     7381 acagtgcgcc ctcagctaac aatgcaatgg tcattatcag tctgccagca aagcgcgcgc
     7441 gcccaaccgg ataggactgt ccgtaggcac tcggcgcaaa actggcggcg atgaggagat
     7501 atgcaagcag aagcatgacg atcgtcaaca tcccgagccg cctgcgctct tcgttggaaa
     7561 tacttcgcgc aggctgagag taataaacat aaaaaagtat ggctggcatc attaccgaaa
     7621 tgatcgttgg cgtagggcgc gttcgcaacg tatcaacaat aaaatcaaag ggataaatga
     7681 tgatcctgtt aatcagttca aatagaccag ggggtggtcg atccaaacga agtgaatttg
     7741 caggagccaa tgctaatacg atcaaggcta aaaccgcccc aacgaaggac caggctaaaa
     7801 gaatcagggc tggtttgcgt acatttttgt ttccccagat ccaggtccaa atgattgcaa
     7861 gaagcagggt tgtgatcatc actgtcgtcg gaggttctga aaagcctcca atgatcaatg
     7921 ggctgatcaa acacactaat tgtatccaaa gcggaggaaa gcgtttctcc gcaaggctga
     7981 tctgccgcaa gaggaatgcg ccgaaaaaag gaataaatac cagcggcgcg aaatgcgagg
     8041 tcatgccagc ccgccagtat agagtttcat acagcgcggg agcctgtagc actgagaaaa
     8101 acgcgacgga agcagccagg aaaaaaatca tccaatgatc ccacctcaaa cggcacatcc
     8161 gtactatttc tttcagaaag aaatacagtc ccaggacaaa tagagcgagc atcaacaccg
     8221 gaaggatcgc aacgttatac cagccaaaga gtagatcaac gagaccaata aagagaatat
     8281 ttgtataacg actcgaagaa acataatatc gacgcgtcat ttcgtcgata agattatcgt
     8341 ttagaaaaaa ggctgagagg caataatcgt cgctcgtgta acgcgagaag attccaagat
     8401 aggcaaatat ccccagcccg atgattgtgg tgacgatccc aagaaaaaga gcgagccaaa
     8461 gccattgctc ttcagaaagt ttttgtctca tagatattcg actggaagac tcatggcttg
     8521 accgccacgg ccaccaggat catgcctatc gcagatttac gattgagttc gcgctccaac
     8581 tgacgctccg cccattttga agaaatcaga gaaaggaacg ggcttaagaa aaaaatcgtg
     8641 cccaggtaat cgattttcaa accggactca atcagcagat tttttagaga cttagaatga
     8701 aagcggctga tatgttgttc gcctgccatc ttcggtgcca tgtttaacat atccactgcg
     8761 cgttccatca gtggccagaa acttcgataa ttgggcgtgg tgaccaaaag tcggccgccg
     8821 gggcgtagca cacgtgctgc ctccttaatg gaaatttgcg gctgttcaag atgttctatc
     8881 acttcagaga gaagaatgca ctcaaaacta tcgcttgcaa gtggtagaag ttcaccaatc
     8941 gattggatgc acatgaaatc gcgcggcgct tctgaccccg actttgcctc ccgcaaacgt
     9001 gaacgaacga acgtcagggc ctgttttgct aagtccaggg cgaccggata actaccgcgg
     9061 acagtggcct gtaaaagcaa attgcctgct ccacagccga cttcaagcaa ctttgagtcc
     9121 gcttttaggt gcgggcgtat caatcgatcg atcatggtta gcttgccgcc atgccaaaat
     9181 cgctgcatgg gatgccccga atgcaacgca cgatattgat aatcacctgc gactgaatca
     9241 taatcaaaag acaattaaca tctcctactc gtactttccc tgcaagtcca tccagcgaac
     9301 catgaagaga ttcgcaaaca tcttaagcga atcattcacc aggcggacgc gacttcgctg
     9361 ccggtgttca atcatgtgca ccgggacagg ctgaatcgtt aattttaatt tgcgtgccac
     9421 gaagagcatt tccacatcaa agccaaaacc attgatactt gtccggcgaa agatttctcg
     9481 ggctgcttca gacttgaagc tcttgaatcc acattgcgta tcgggaagtc ctgaaaataa
     9541 cactgcctga accatccagg aaaaaatctg accggcaata taacgataaa tgggcactcc
     9601 ccttacttcc gagccgggca acacacgtga cccaactgcc agatcaaaac cactttttag
     9661 ggcacttaaa aaattagcaa tcgcatccaa ttcatacggc agatctgcat cgatgaaaat
     9721 aatatgctgc ccgcgacttt ccatcacacc atggcggatt gagaatccct ttcccatatt
     9781 ctgctgattg ctgagaaggc gtagattgat gccgcttctt ttctgccatt cctgaacaca
     9841 aaggacagtt ttatcctggc tcccgtcgtc cacaacgata acctcatatt gttctgttcg
     9901 agccgacata tacactcgca gactctccaa cgtgtttacg atcagggctt cttcattata
     9961 cgccggtatg atgatggaaa gatccagggc tgtcatgatc cactataacc cccaccaagt
    10021 ggactccgca gattaaatat cacataacca tccccctggg catggataag atatgaacta
    10081 aacaatctgt ttttcaggtc caactggcgg tcaagttcgt caaaatctgt tacgagaaaa
    10141 aaatctcttt gtgaagaata cttttcaaac agctcatcaa aagaaaaact gcccccacgc
    10201 gtatcaatat agttgatgtc tcctacatac ggccaggtgg cagcagtttt caatccccaa
    10261 tattcaagcc gcgaaccata gtcctgcgtg agcgcaacca cgcgttcatc ggcaagccct
    10321 tcacccactt cagcccacat cgcagcttca gggcgataat ccacagcctt cagttgattg
    10381 cggatatccc acaggacaga agatagacca aggatcagta tgaatgatgc cgcagttcgc
    10441 atccatggac gtgggcttga ctccgctaaa cgcccgagaa accaatctcc aagcggactc
    10501 aacgacactg ccacaatggg aatcaatacc agatgatagt aatcatgggt agcgacatga
    10561 taattaaaga aaagaccgta gaacaggtag gcaatccata acccacgcat gagacttcgc
    10621 agccggactt cctttactaa aagaagtccg agcaagccca gcatgatgaa cagaccgcca
    10681 gcagccaggg ttgcctttga accccataac acataattta cgggacttaa caataattcc
    10741 ggaatgaagc gtccgctgaa ctgcgcaccc aggtaaccgc cgataaaaat tccgtaaatg
    10801 agatagatcg cagcaggcag aattccaatg actgccatca gccacacgtg aggatgtttc
    10861 aacaattcac gcagcgtaaa tcgcgagagc gcaagtccca acgccgggcc gatcacgaaa
    10921 aaagcggccg agaatttaat gagaatcgcg agtccgccta aaagtcccgc gagaagcgcc
    10981 caccgccatc ctttgcctct cgccgcttcg tcttcgctaa cgctccgctc agcgccaatt
    11041 gaagaaaggt caaaccaacg tgaaaatgcc caccagaaag ccaagatgaa catcaccatc
    11101 aaaggatccg gttgaaagct tcgacttgca agaacggaat aagggaaaaa taaataatat
    11161 gctgtggcga aaatcgcgcc gtcaaacgag ctcaaatcac gcacaagcag gaagaggaat
    11221 aatcctccag ccagccagaa cagggaagaa taaatacgcg caatccaaac ctgctccccc
    11281 gtaaatcgat aggtgaatgc gaccaggcgt tcaaaaatca ccggctccac atccgctttc
    11341 agcttcgcct gacggatggc ggtttccaat cgctgggtgg aaatgccatc aggctgagtt
    11401 tcataataca aggcgcgggc ttttatcgcg gaaagcaatt gacgggtcgg gtgaaaatca
    11461 agggggagat ccgttagatc atacaggcga attgttagag caaggctaaa aagcagtaca
    11521 agcgcgatgc cacgtgatac tggcgacgaa aaaaaagatg gctgggacat gagcccgtgt
    11581 attttatcag attcgatttt tttacttatc ggacgagtaa ccccgctgtc ggcgacgcaa
    11641 aatgaactct cgtagggcat ttaaaccagt tagattcagc aaggaagcaa ccagtatgtt
    11701 tagccgtgca agggccgtag gcggatggat gaagaatgca cgcatccagg ccttcactgc
    11761 cgaccagggt tgtcctccat cgagacggta acgcgcatcc acgcggtgag cggaggcccg
    11821 ggcgcgcctt tctacaggcg aaatcacacc agccagttta gattgactct ttgcccaatc
    11881 taagatacgg aaggcttcgc gcccaaattc cgcggctttg gctcggtttt ttgcctcggc
    11941 atgataacgt gcagccgcgc tggtttgagg aacatgcaaa atccgtccct gctgtgcaag
    12001 acgaatccag aggtgatgat caagtagaaa gtggaagcca gtatcgagtc tgcctgcttt
    12061 ttcaaatgca gcgcggcgaa agaatacagc aggctgaccg atgatttgaa aacataagag
    12121 atcttccaaa gagagttgtt tgtatttcaa attgttaatg gtctgt
//
LOCUS       DASTJP010000886         9006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_794091, whole genome shotgun sequence.
ACCESSION   DASTJP010000886 DASTJP010000000
VERSION     DASTJP010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794091"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFQ23_25740"
     CDS             complement(<1..702)
                     /locus_tag="VFQ23_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9910075.1"
                     /translation="MTNPNWWQTAVFYQIYPRSFADGNGDGIGDFPGMIEKLDYLADL
                     GVDALWLSPHFPSPQFDAGYDISDYVNVAPEYGTLDDFKRFLDGAHERGMRVILDLVM
                     NHTSHLHQWFIESRSSRDNPKRDWYIWRDGKNGGPPNNWLAPFGGTAWEYDEVTGQYY
                     YHYFLKEQPDLNWRNPEVKKAMWDTVRFWLDMGVDGYRLDAISTIFEDPDMPDHNGIL
                     SDVLRRKLNHGFPLGT"
     gene            complement(728..1108)
                     /locus_tag="VFQ23_25745"
     CDS             complement(728..1108)
                     /locus_tag="VFQ23_25745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910076.1"
                     /translation="MNQEHTGPEFPPSERMPQRISEGMIVHDAAGESIGTVQVVYFGG
                     ASEEAIARVFRPESGESDLSNLPEELVTHLMSHGYMIVEGPDLAGAKRYIRPEQIEGV
                     FPVEIAGTVTDIVRLRVTRNELTQ"
     gene            complement(1138..1473)
                     /locus_tag="VFQ23_25750"
     CDS             complement(1138..1473)
                     /locus_tag="VFQ23_25750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910077.1"
                     /translation="MDFSGLLYLLPVCIVGLLVIALFWWIFGWLLTESVDRNLRRCPN
                     CKRGGAGKIIDTEIESLGVKVDRSKLTTIRTKREKVTDRYACDHCGHQWTRSFTREEQ
                     LPVQKASKI"
     gene            complement(1605..4388)
                     /locus_tag="VFQ23_25755"
     CDS             complement(1605..4388)
                     /locus_tag="VFQ23_25755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9910078.1"
                     /translation="MRSTLSTTADDLRPYFSEGAKIILGFFLVAAVVIFSLHTFLSVA
                     YRYSLDYGEAPLIDQAIRLSRGENIYRSDLSTPPYTIANYPPLYVISLIPFLSWFDSP
                     FHMARVISVIATLLSATFISLIVYQLSKNRYAALITALLFLANPYVVQWSVRARIDLL
                     ALAFATGALFVLVRWPKERWSWIVGGLLLVAAIYTRQSYALAAPLASFIWLWTQDKRR
                     AIWLALFVGGIGIALFLLINTLTDGGFYYNIITANVNEFSWGRLGQNLIQVWRDNYII
                     LTLSGLFLFFGWRSQRSWPLVAFFLVGALLSALTAGKIGSNVNYFLELAAALALVGGA
                     LVVWSEPKPWPVTAVLLLIALQFGMSLRASLQLNVDWILSQRYFDFNAMQLLEQEVKE
                     MDGVVLADEYMGLLTMNDRPIYLQPFELSQLANAGMWDQQPLLNEIKDQNIDGILIHH
                     FDTWPVHKERWTPELLAAIDEYYRPVQTLAGTVVYIPRGESSIAMVPSPTNGSGAAPT
                     LVWDGSPISISPAAFVSEVSISIDPTNPDQLAATTTRTSKQDCEQPNCIIQLLLFTSQ
                     DGGENWEERATFGQQQVAVADGQVAFDPKGVLNTLAFRNGVVVLSQTTFAEADLETLA
                     GFADVTSALVGAKPWLRVHPETGELFMTFDAQEGDRLYVTPSLKRSNDGLLWSLTARA
                     DQHISATDIFSPRGTGPQDIQVLIGTGNNVSLVWVWDPEPWSWPRTVWMSNSSDGGET
                     FGEPAPILKTWGPINTSSANGNFAIVYKTGDETNQQLAVATTSDNGQSWTSTFANGNV
                     PLYFDPDKGPGISMSDENSIDLVFYAMDNPDQNCTLTVESWQQTMLSGRIDPCTYNVY
                     FTSSSDGGLSFAEPIQLNQQPIHGKDFARFQGTSRPGSHLAVASGIEFAYPMWIGTPE
                     TNKTQIYSAKIAR"
     gene            complement(4399..4743)
                     /locus_tag="VFQ23_25760"
     CDS             complement(4399..4743)
                     /locus_tag="VFQ23_25760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910079.1"
                     /translation="MTMSSDGILETKVGPRVVALAYGLWFITSILAVLSYLAGREMVL
                     RNYVRFFPTGQGSFAAVNSITSVAMAILPIAIIIGGFEYQHRNVGKPGAWVMLARTFA
                     VEIGMLLLAFYI"
     gene            complement(4754..5521)
                     /locus_tag="VFQ23_25765"
     CDS             complement(4754..5521)
                     /locus_tag="VFQ23_25765"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9910080.1"
                     /translation="MNTKHITTENLTGYIHQTLDDAQREEIDAHLVECPTCRASLAQQ
                     EIQYRQISNELVSILNFTTPSPQMNFSAIAPRLHRHISQNILLDRAIFVPMTLALTGF
                     VFALFGLWRAIGNQAFTSPAQPLGVFPPLACFFLVLASVQQSDSSYSPRWSRRAITWI
                     IVLILWLGSAFIGLLDIIALSDLAIIAVIAMGGQKATAEPVAMTAVLLGALLYVGFII
                     GGAEFHYRNVGQPRSWKLLSITLLIQLFILILPYLLR"
     gene            complement(5518..6096)
                     /locus_tag="VFQ23_25770"
     CDS             complement(5518..6096)
                     /locus_tag="VFQ23_25770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9910081.1"
                     /translation="MQNLEALIERWRGGDQQAAESIYNQHREQTFRLAYALLGNTEDA
                     EEAAQDALTYALLHIHQFDPRRSKFTTWLHTITVSRSRDILRKRRLPTFPLIALFQNG
                     RTVEDSLPGPEQRTSSNETRKSVWDAIQHLSPLLREAAVLRYWGNFTYQEIAEILGCT
                     MKTAQSRVRLAHLKLASVLGENKLQDTAEETR"
     gene            6171..6353
                     /locus_tag="VFQ23_25775"
     CDS             6171..6353
                     /locus_tag="VFQ23_25775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910082.1"
                     /translation="MPKFEVGPFIQSIIVALIFFAIGRYITIQTDGAGRWLIFAALII
                     FYAVWWVWYTRGKRKK"
     gene            complement(6463..6897)
                     /locus_tag="VFQ23_25780"
     CDS             complement(6463..6897)
                     /locus_tag="VFQ23_25780"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9910083.1"
                     /translation="MAKPDDTVNFLVTIPLFQGLNNKQLNQLAKRFVPRSYESGKNIV
                     TQGKGGEGMFVMVSGRAEAVVESSDGSKTKVNDFAARDFFGEVAMLDDGPRTASVTAT
                     ADTECLVLTRSDLIALMKMDADMGIEIAIALAKRLRRVASMM"
     gene            complement(6920..7984)
                     /locus_tag="VFQ23_25785"
     CDS             complement(6920..7984)
                     /locus_tag="VFQ23_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9910084.1"
                     /translation="MSDNNGNSQKYDILLDVRGLKKYFPITGGLMRKVVGYVKAVDDV
                     NFFVREGETLGLVGESGCGKTTAGRAVLRIYEPTDGEIRFRSRALSGTGKAEWVNIRE
                     LDKEQLKTVRQDISMIFQDPIGSLNPRMTVYDIITEPLVIHSKMAPDTEDYVVNLLER
                     VGLRPEHIRRYPHEFSGGQRQRIGIARSLALNPQLIICDEPVSALDVSVQAQTLNLMN
                     DLKADFNLAYIFVAHDLSVVQHISDRIAVMYVGRVMEMADSLALYEKPLHPYTEALMS
                     SVPRPDPRLKKDRIIMEGDVADPGNPPPGCLFHPRCRYAQERCKVEVPELRELKPDHF
                     VACHFAETLDLKGVADLTAV"
     gene            complement(7999..>9006)
                     /locus_tag="VFQ23_25790"
     CDS             complement(7999..>9006)
                     /locus_tag="VFQ23_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9910085.1"
                     /translation="TLGIIGESGCGKSITAKAIMNMVPKPGKMRGEITFYQKSDDGEI
                     IDQVRLDQTDPDGEIIRKIRGGHIAMIFQEPMSSLTPVYSTGFHLMEAIKLHRFMPGE
                     TIGGEMTRNLQKKQQVTEEEAKKIAIDMLRRVGIPNAAKRFDSFPHQLSGGQRQRVMI
                     AIALSTSPDLLIADEPTTALDVSIEAQILDLMRDLQENANMAIMFITHNLGVIAEIAD
                     EVVVMYMGKEVERATTIELFERPKHPYTTALLGSIPNIGEKKGKLETIAGMVPSPFNL
                     PSGCVFHPRCPAFMPGKCDRVAPNYAEVEKDHWARCLLYDPCWPNTAEADSIKNQAVA
                     G"
BASE COUNT         2293 a         2274 c         2253 g         2186 t
ORIGIN      
        1 tgtgccaagt ggaaacccat gattcaactt gcgccgtagt acatcgctca gaattccgtt
       61 atgatccggc atatctgggt cctcgaagat ggtgctaatc gcgtccaaac ggtaaccatc
      121 cacgcccatg tcaagccaga agcgtactgt atcccacatc gcttttttga cttcagggtt
      181 acgccagttg aggtcgggtt gttctttcag gaagtagtga tagtaatatt gtcctgtgac
      241 ttcgtcatat tcccacgccg taccgccaaa aggggcaagc cagttgttgg gtggacctcc
      301 attcttgccg tcgcgccaga tgtaccagtc gcgcttgggg ttgtcgcggc tggaccgaga
      361 ttcaatgaac cattggtgca aatgggaagt gtgattcata acgagatcca ggatgacgcg
      421 catcccacgt tcatgcgcgc catcgaggaa acgcttgaag tcatcaaggg tgccatattc
      481 aggagcgacg ttgacgtaat cggaaatatc gtagcccgcg tcaaattggg gagaggggaa
      541 gtgcggcgag agccacagcg cgtcaacgcc cagatcagcg agatagtcaa gtttttcgat
      601 catacccggg aagtcaccga ttccatcgcc attgccatct gcaaaactgc gcgggtaaat
      661 ctggtagaac actgctgtct gccaccaatt ggggtttgtc atttgatttt cctgggaaaa
      721 aatttggcta ttgtgtaagc tcgttgcgcg tgactcgtag tcgtacgata tcagtgactg
      781 tgcctgcaat ttctacaggg aaaacgcctt cgatctgctc gggtcttata tagcgcttcg
      841 caccggcgag atcaggtccc tcaacaatca tatagccgtg actcatcaga tgtgtaacca
      901 actcctccgg cagattactt aggtcgcttt cacccgactc gggtcgaaaa acccttgcga
      961 tggcttcctc gcttgcaccc ccaaaataaa cgacctgtac ggtaccgata gactcaccgg
     1021 ctgcatcgtg aacgatcatc ccctcactaa ttcgctgcgg catgcgctct gaaggaggaa
     1081 actctggtcc agtgtgttcc tggttcatgt tattgtcctt tcttgggaat atctgattta
     1141 gattttcgat gccttttgta caggcaattg ttcctcacgc gtaaatgaac gtgtccattg
     1201 atggccacaa tgatcgcagg cgtagcgatc cgtcactttt tctctttttg ttcgaatggt
     1261 ggtcagcttg cttcgatcga cttttacacc gagtgattca atctctgtat cgattatctt
     1321 ccccgcgccg ccgcgtttac agtttgggca gcgccgcaaa ttgcgatcca cagactcggt
     1381 aagcagccag ccgaaaatcc accaaaaaag tgcaatgacc agcaaaccta cgatacaaac
     1441 cggaaggaga tagaggagcc ctgagaaatc cattgagtct ctagagtttc acgtacaatt
     1501 ttcgtgagga ttgtatcatt attaataacg taattaccgc cgtagaatta cgcaaagatt
     1561 ccagttctgg aggtgattca cagcattccg atggaaaaaa attcttatct tgcaattttc
     1621 gcgctgtaga tctgagtctt attggtctcg ggtgtaccaa tccacatcgg ataggcaaac
     1681 tcgatccctg aagcaacagc taaatgggat ccgggacgtg aagttccctg aaagcgggca
     1741 aaatccttgc catgaatagg ttgttgattc agttggatcg gttcggcaaa ggaaaggccg
     1801 ccgtcactgc tggatgtgaa gtacacatta taggtacacg gatcgatccg accagaaagc
     1861 atagtctgct gccagctttc aaccgtcagc gtacaatttt gatctggatt atccatcgcg
     1921 taaaagacca ggtcgattga attctcgtca gacatgctaa tacccgggcc cttatccgga
     1981 tcaaagtaca atggaacgtt tccgttggcg aaggttgagg tccaggattg gccattatcc
     2041 gatgtggttg caacggcaag ctgttggttc gtttcatcac cggtcttata tacgatagcg
     2101 aaatttccgt ttgcggaact ggtgttgatg ggtccccagg tcttcagaat cggagctggc
     2161 tctccgaaag tttcaccacc atcacttgaa tttgacatcc agaccgtgcg tggccaggac
     2221 cagggttcgg gatcccacac ccacacaaga gaaacgttat tcccagttcc aatgagcact
     2281 tgaatgtcct gcgggccagt gccacgcggc gaaaaaatat cggtggcaga aatatgctgg
     2341 tctgcgcgag ctgttaacga ccaaagcaga ccgtcgtttg agcgcttcaa gctgggggtc
     2401 acatacagcc gatcaccttc ctgggcatca aaggtcatga agagttcacc ggtttcagga
     2461 tggacccgta gccagggctt tgcacctaca agggcgctgg tcacatcggc aaagccggcc
     2521 agtgtttcga gatcggcctc cgcgaaagtg gtctggctga gaacaaccac gccattgcga
     2581 aaggcaagcg tattcaagac acccttgggg tcaaaggcta cctgaccatc agctactgcc
     2641 acttgctgct ggccaaaggt agcgcgctct tcccagtttt caccaccatc ctgtgatgta
     2701 aacagtagga gttgaataat gcaattcggt tgctcacaat cctgcttgga tgttcgcgtg
     2761 gttgtagcag ctaattgatc cgggtttgta ggatcgatcg atattgagac ttcgcttaca
     2821 aaagcagccg gacttatcga aattggtgac ccatcccaaa cgagagttgg cgcggcaccc
     2881 gacccattgg tgggtgaagg aaccatcgcg atgctgcttt cacctctggg gatatacacg
     2941 accgtacctg ccagcgtctg caccggacga taatattcat cgatcgccgc gagcaactcc
     3001 ggggtccagc gttccttatg aactggccat gtatcaaaat ggtggatcag aatgccgtca
     3061 atattttgat ctttgatttc gttcagcaga ggttgttgat cccacatccc ggcattggcc
     3121 aattgcgata gctcaaatgg ttgtaagtaa atgggccggt cattcattgt caacaaaccc
     3181 atatactcat cggccagcac cacaccatcc atttccttga cttcctgttc gaggagttgc
     3241 atcgcattaa aatcgaagta gcgctgactg agtatccaat ccacgtttag ttgcaatgag
     3301 gctcgtaatg acataccaaa ttgaagcgcg atcaacagga gcaccgccgt gacaggccaa
     3361 ggtttaggct cactccaaac cacaagcgcc ccgccaacca gcgccagggc agcggctagt
     3421 tccaggaagt aattgacgtt cgacccaatt tttccagcag ttaacgctga aagtaacgcg
     3481 ccgaccagga aaaacgctac cagcggccag gatctctgcg agcgccaacc aaagaataag
     3541 aacaagccgc tcaatgtcaa aataatatag ttgtcccgcc aaacctggat gagattctgc
     3601 cccagcctcc cccaggaaaa ttcattaaca ttggcggtga tgatattgta gtaaaagccg
     3661 ccatccgtga gagtattgat taaaagaaat agagcgattc caatcccacc cacgaacagt
     3721 gccagccaaa tggcgcggcg tttgtcttgg gtccacagcc agatgaaact tgcaagaggc
     3781 gcagccagcg cataggattg gcgcgtataa atcgcggcta ctaataacaa accaccgaca
     3841 atccaactcc agcgctcctt cggccagcgc acaagtacaa ataaagctcc agtagcgaac
     3901 gccaaggcta ataggtcaat acgcgctcgg acggaccatt gaacaacata aggattggct
     3961 aagaaaagga gggcagttat caaagctgcg taacgatttt tgcttagctg gtagacaatg
     4021 agactaatga acgttgccga aagtagagtg gcaatcacag agatcacgcg cgccatatgg
     4081 aaaggtgaat caaaccaact caggaacggg atcaggctga tcacatacag gggtggataa
     4141 ttggcaatgg tgtagggggg cgtgctgagg tctgatcgat agatgttttc gcctcgagaa
     4201 agacgaattg cctggtcaat caggggcgct tcgccgtaat caagggaata acggtatgcc
     4261 accgacagaa atgtgtgtag agagaaaata actacggctg ccaccaggaa aaaacctaaa
     4321 ataattttcg cgccctcgct gaaatatggg cgcagatcat ccgctgttgt agagagtgtt
     4381 gaccgcatgg cgatttcctt agatgtagaa cgctaaaagg agcatgccaa tttccaccgc
     4441 aaatgtgcgg gctaacatca cccatgctcc aggtttgcct acgttgcgat gctggtactc
     4501 gaagccgccg ataataattg caatcggcaa aatggccatc gcaaccgaag tgatggaatt
     4561 gacggccgca aaacttcctt ggccggtggg aaagaagcgc acataattgc gtagaaccat
     4621 ctcacgcccg gcaagatagg acagcacggc caagatcgat gtaataaacc aaagaccata
     4681 cgctaacgca acaacgcggg ggccgacttt cgtttcgaga attccatctg aactcatagt
     4741 cattgtagat ctcctatcga agcaggtaag gcaaaattag aataaataat tgaatcagca
     4801 gggtgatgct taataacttc catgagcggg gctgacctac gtttctataa tgaaattcag
     4861 ctccgccaat gatgaatcca acataaagca acgctccgag cagaactgcc gtcatagcaa
     4921 ccggctccgc ggtagctttc tgcccgccca tggcgataac tgctatgatc gccagatcac
     4981 tcaacgcgat gatatcaagc aggccgataa acgctgaccc aagccataag atcaaaacga
     5041 taatccaggt gattgcccgc cgagaccagc gcggcgaata cgaactgtca gattgctgta
     5101 cggaggccaa gaccaggaag aaacatgcca gcggtgggaa gactcccaat ggctgtgcag
     5161 gagaagtgaa ggcctgattg ccaatggccc gccataatcc gaacagggca aatacgaatc
     5221 cggtcagagc tagagtcatc ggcacaaaaa tagctctatc aagcaggatg ttttggctga
     5281 tgtgtcggtg caaccgcggc gcgatcgcag aaaagttcat ctgtggggag ggggttgtaa
     5341 agttcaatat ggagactaac tcatttgaaa tctgccggta ttgtatttct tgttgagcca
     5401 gactggctcg acaggttggg cactccacca ggtgagcgtc gatctcctcg cgctgcgcat
     5461 catccagggt ttgatggata tatccagtta aattttccgt agtgatatgt ttcgtattca
     5521 tcgcgtttcc tccgcggtat cttgaagttt attttcgcct agtacactgg cgagttttag
     5581 atgggccagc cgcacacgcg attgagccgt tttcatggtg cagccgagaa tttcggcaat
     5641 ctcctgatag gtgaaattgc cccagtaacg caggacagca gcctcccgca ggagagggga
     5701 caagtgttga atcgcatccc acaccgattt gcgagtttca ttacttgaag tgcgttgttc
     5761 cggtcccgga agggaatctt ccacagtgcg cccgttctga aacaaagcaa tgagagggaa
     5821 agtcggtaga cggcgtttgc gaaggatatc ccggcttctg cttacagtaa tggtgtgcag
     5881 ccaggtggtg aacttactcc ttcgcggatc aaattggtgg atatgaagca gtgcgtatgt
     5941 cagggcatcc tgggcagctt cctcagcatc ttccgtattt cccaataagg cgtacgccag
     6001 acggaacgtt tgctccctgt gctggttata gatcgattcg gctgcctgtt gatcgccgcc
     6061 gcgccagcgt tctatcagcg cttcgagatt ctgcatagtt attccaacat ctttctatga
     6121 tattatacgc agacctgttt ggttttgctc acagcaggaa aggaaaaaac atgcctaaat
     6181 ttgaagtcgg accctttatt cagagcatta tagtcgccct gattttcttt gcgatcggaa
     6241 ggtatattac catccagacg gatggcgccg gcaggtggtt gatctttgcc gctctgatta
     6301 tcttctatgc agtctggtgg gtttggtata caaggggaaa aagaaaaaaa taatcaattc
     6361 tgttgatatc tctcccatga tatacaaaca cccatgcgct ggacgatgac gtcagaccgc
     6421 atgggtgttt cgtatacaag gcatccaaca aagttggcct actcacatca tactcgccac
     6481 tctccgcagg cgctttgcca gcgctatagc aatttcgatg cccatatccg catccatttt
     6541 catcagggcg atgaggtcag agcgagttaa cacgaggcat tcagtatctg cagtggcagt
     6601 gacagaggct gtccgtggtc catcgtcaag catagcaact tcaccaaaga aatctctggc
     6661 agcaaaatca ttgaccttgg ttttgctgcc atcgcttgac tcaaccactg cttccgcgcg
     6721 gcctgatacc atcacaaaca taccttcacc accttttccc tgggtgacga tattcttacc
     6781 agactcgtag cttcgaggta caaacctctt cgcaagttga ttaagttgtt tgttgtttag
     6841 accttgaaac agcggaatcg ttactaaaaa attaactgta tcatcaggtt ttgccattgt
     6901 atgactcctt aaggagagtt tatactgcgg tgaggtcagc aacacccttg agatcaagtg
     6961 tttctgcgaa gtggcaggca acaaaatgat ctggcttgag ttctcgcaat tcagggactt
     7021 ccactttgca gcgttcctgc gcgtatcggc agcgcgggtg gaacaaacat cccggcggag
     7081 gattgccggg gtcagccacg tcaccctcca taataatacg atcctttttc agccgcggat
     7141 cgggccgtgg aacggaagac atgagtgcct cggtgtaagg gtgcagaggt ttctcataga
     7201 gtgcgagcga gtcagccatt tccataacac gaccaacata catcaccgcg atacgatcgg
     7261 agatgtgttg cacgacacta aggtcgtgag ccacaaaaat ataagccaga ttaaagtcgg
     7321 ctttgaggtc gttcatgaga ttgagggttt gggcttgtac tgacacgtcc agcgcggaca
     7381 ccggctcatc gcagatgatc aattgtggat tcaatgccag cgagcgggcg ataccgattc
     7441 gttggcgctg accaccggaa aactcatggg gatagcggcg aatgtgctcg ggtcgcaacc
     7501 ccacgcgctc taacaggttg accacataat cttccgtatc cggagccatt ttactgtgga
     7561 tgaccaaagg ctcggtgata atatcgtaga ccgtcatacg aggattcagt gagccaatgg
     7621 gatcctggaa gatcatcgaa atatcttgtc tgacagtttt gagctgctct ttgtccagct
     7681 ctcgaatgtt gacccattcc gccttgcctg tacctgaaag cgcacgtgac cggaatcgaa
     7741 tctctccatc ggtcggttca tagatgcgca acacggcgcg cccagcggtg gttttgccac
     7801 aaccactttc gccgacgagt ccaagtgttt caccttcacg tacgaagaaa ttgacatcgt
     7861 caaccgcttt gacatacccc acgaccttac gcataaggcc gccggtgatc ggaaaatatt
     7921 tcttcaggcc tcgaacatcg agaagaatat cgtatttttg ggaatttccg ttgttatcgc
     7981 tcatcgtatc cttcctcttt agccagcaac tgcctgattc ttgatcgagt ctgcttctgc
     8041 tgtgtttggc cagcagggat catacaacag acaccgcgcc cagtggtcct tttcaacttc
     8101 cgcataattg ggagcgaccc ggtcacactt acccggcata aaagccggac aacgcggatg
     8161 gaagacacaa ccagagggta agttgaaagg gctgggaacc atccccgcga tggtttccaa
     8221 tttgcctttc ttctcaccaa tgttgggaat ggaaccaagc aaagcagtcg tatagggatg
     8281 tttgggcctt tcaaacaact caatcgtagt ggcgcgttct acttccttgc ccatatacat
     8341 gaccacgacc tcatccgcga tttccgcgat gacccccagg ttgtgggtga tgaacatgat
     8401 cgccatgttg gcgttctctt gaagatcacg catcaggtcc aggatttggg cttcgatgga
     8461 aacatcaagt gccgtggtcg gctcatctgc aatcagcaga tcgggggatg tggaaagagc
     8521 aatggcgatc ataacgcgct gtcgctggcc gccgcttaac tggtggggaa agctatcgaa
     8581 ccgcttggcc gcattgggga tacctacccg gcgaagcata tcaatagcga ttttcttggc
     8641 ttcttcttct gttacctgtt gtttcttctg gagattacgc gtcatttccc cgccaatggt
     8701 ttccccgggc atgaaccggt gcaacttgat cgcttccatc aggtggaacc cggtactgta
     8761 aaccggcgtg agtgaactca tgggttcctg aaagatcatc gcgatatgac cacctcggat
     8821 tttgcgtatg atctcgccat ccgggtcggt ttgatcgagg cgaacctgat caatgatctc
     8881 accatcatcc gatttttgat agaaagtaat ttcgcctctc atcttgcccg gctttggtac
     8941 catgttcatg atcgctttgg cagtgattga tttcccacac ccactttcac cgatgatccc
     9001 taacgt
//
LOCUS       DASTJP010000887         4676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_720188, whole genome shotgun sequence.
ACCESSION   DASTJP010000887 DASTJP010000000
VERSION     DASTJP010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720188"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="VFQ23_25795"
     CDS             <1..1536
                     /locus_tag="VFQ23_25795"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9910086.1"
                     /translation="IQAGISLTTLLDPQAAVQEVAHIARKTLEARFVFVTLLDQQGNF
                     SRTAFAGSAPLLLSSFDEDISNEPLMQAALNAVKPFRVRDVRKYANTKKLEIDHAGLR
                     SALAIPIRLHRLSIGTILAFGKQDGVFFAESDESLADLLSSQAAASIESSWLYQELRN
                     TLNITSMLYQLSVDVIQTEELSNAAELIAQAAHKVTNSRETGIVLLTRDGQVEAEVEI
                     DANGLHTRREHPMENIGQAIQTGQSIIVPVENGSLVCYPLLTRGRTYGALWMSIPESR
                     GQNFANMQTLANQAAIALERSILLSEFRQQAKQIEAAYAELEVTYDRTLTALMSALDA
                     RDRETEGHSMRVSLLACLLAEEIGLSGLQLKSLERGALLHDIGKIGISDTILHKPGKL
                     TEDEWKIMRIHPDIGAKIVEGIPFLQETLPVIRYHHERWDGSGYPLGLKGKDIPVQAR
                     IFAVADVFDALTSSRSYRKRSSPEDAVQYLREQAGILFDAEIVEALARVPYKEFTEVP
                     SAA"
     gene            1623..3167
                     /locus_tag="VFQ23_25800"
     CDS             1623..3167
                     /locus_tag="VFQ23_25800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET9910087.1"
                     /translation="MKNRKPISTFAPIEVRRFILPLTITTILFMMGIILNGTLVKPIN
                     VSMIVYGVAWIAITLIYDFIIAKSTVAYRLFGWIYAITSIIGLGFLYFILPPSLLEVF
                     YLMIIFSTVSLTTVSGRYQAYFTLLGILAITLPSESQHFTNTKSILDYFIPFVTAIMV
                     VEAVLQIKETTQRHIHRLETINKISRQIMLSLNTEETIALLATAMQDAVEADTYFVGI
                     VRDNEIHLDLFHDDGEHFNGTRIPIDGTLSGWVIKNQKELFLNDLRKDIKLDGLESLV
                     SGSERTSLSWMGVPLSASNVVGIMALSSYQPNAFDRADMELLSNLAQHVTLALNNAFQ
                     HERVEEQARLDSLTGVYNHGYFLRKLAEQADGSSHSGIPLSLIMLDIDYFKQYNDTFG
                     HLVGDQILRTLCRAITQHIKQTDAVGRWGGEEFIISLPGASGIQAMEVAQRIGQTMSG
                     LQVEDMDQKKVPVPTVSQGIAVFPEEASEIYRLIDLADQRLYVAKEQGRNQIEPKSFQ
                     RERIPH"
     gene            complement(3204..4241)
                     /gene="ltaE"
                     /locus_tag="VFQ23_25805"
     CDS             complement(3204..4241)
                     /gene="ltaE"
                     /locus_tag="VFQ23_25805"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HET9910088.1"
                     /translation="MEYIDLRSDTVTKPTPEMREAMAEAEVGDDVYGDDPTVNDLEEI
                     AAGMLGKEAALFVPSGTMGNLIALLVHCQRGDEVIVGSRSHIYLNEAGGMSALGGIQP
                     CPIQNQPDGTLAVEDILTSIRTEDVHHPITRLICLENTQNVCGGVPLSGDYTRQIGEL
                     AHENNLLLHIDGARIFNAAVAQNVSVATLVEPADSVMFCLSKGLASPVGSMVLGTRKF
                     IARARHLRKMLGGGMRQVGILAAAGIISLEQMTKRLGEDHTRAKKMADGLRNINGLNL
                     DPNSPYTNMVYLNISDNAGQDLFQIGEQLKGLGILIDADNAKRLRLVTHYWIDDAAVE
                     KTVSAFHEVLN"
     gene            complement(4244..>4676)
                     /locus_tag="VFQ23_25810"
     CDS             complement(4244..>4676)
                     /locus_tag="VFQ23_25810"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9910089.1"
                     /translation="RKVEKTYLALVDGRPPTPSGRVETFIGRDPRHRKQMAIVPESRG
                     REAISEYKTKENFKDHTLLDFHPLTGRTHQIRLHCSFLGCPIVGDKIYGRKKISIAIK
                     RHFLHAYRLKIVLPGEAEHRVFEANLPDDLLSVLNTLRKES"
BASE COUNT         1242 a         1128 c         1110 g         1196 t
ORIGIN      
        1 atacaggctg gtatttctct aacgacgctc ctggatccac aggccgcagt tcaggaagta
       61 gcgcatatcg ctcgcaagac actggaagct cgttttgtat ttgtcacact gctcgatcaa
      121 cagggaaatt tttcgcgcac cgcatttgcg ggcagcgctc cgcttttgct ttcctcattt
      181 gatgaagata tttccaacga acccttgatg caggccgcgt taaacgcagt gaagccattc
      241 cgggttcgcg atgtacgtaa atacgccaat acgaaaaaac tggaaattga ccatgcaggt
      301 ttgagaagtg cgttggcaat cccaattcga ttacaccgcc tgagcatcgg aacaatctta
      361 gcgtttggaa agcaggatgg ggtttttttc gccgagagcg acgaatcact ggcagactta
      421 ctctcctcgc aggctgccgc ctcgattgag agttcatggc tatatcagga gctgcgtaat
      481 acgttgaaca ttacctctat gctctatcaa ttgagtgtag atgtgattca aactgaagag
      541 ctaagtaacg cggcggagct gatcgcgcag gcagctcaca aagtgaccaa ctccagagag
      601 actggcatcg tccttttgac gcgcgacggg caggtggaag ccgaggttga aattgatgca
      661 aacggactgc atacacgacg cgagcatccc atggaaaaca tcggtcaggc gattcagacg
      721 ggtcaaagta ttatcgttcc ggtggaaaat ggctcactgg tctgttatcc acttcttacg
      781 cgcggtcgca cctatggcgc gctgtggatg agtattcccg aaagtcgcgg gcaaaatttt
      841 gcgaacatgc agactctggc aaaccaggcc gccatcgccc tcgaacgctc gatccttctt
      901 tcagaatttc gccagcaagc aaagcagatt gaagcagcgt atgccgagtt ggaagttaca
      961 tacgatcgta cgttgaccgc attaatgtct gcattggacg cgcgcgaccg agaaacggaa
     1021 ggtcacagca tgcgcgtcag ccttttggcg tgtttgttag ccgaagaaat cggattaagt
     1081 ggactccagc tcaaatcgtt ggaacgcggt gcattactcc atgatatcgg caagattgga
     1141 atcagcgaca cgatccttca taagccgggc aagctgactg aagatgagtg gaagatcatg
     1201 cgaatccatc cggatatcgg tgcgaagatc gtagaaggga ttccatttct acaggaaact
     1261 ctgccggtga tccgctacca tcatgaacgc tgggatggca gtgggtatcc tctcgggtta
     1321 aagggaaaag atatccccgt acaggcgcga atattcgccg ttgcagacgt gttcgacgcg
     1381 ctcaccagca gtcgcagtta ccgcaagaga tcctctccag aagatgcagt gcaatatttg
     1441 cgagaacaag ccggcatcct ctttgatgcc gaaattgtgg aggcccttgc acgagttccc
     1501 tataaggaat tcactgaagt accaagtgcc gcataattct caacctgaat ttacgaacat
     1561 gagtcaaaaa atttgttcca acgtttactc aatcgcgcaa caagtggtgg aaaagcccct
     1621 gtatgaagaa tagaaaacca atttcgacct tcgcgccgat tgaagttcgg cgttttattt
     1681 tgcctctcac tattacgacg attttattta tgatgggtat cattttgaat ggtaccctgg
     1741 tgaagcccat aaacgtctca atgatcgttt acggggtagc atggattgcc atcacattaa
     1801 tctatgattt catcatcgca aaatcaaccg ttgcctatcg attgttcggc tggatctatg
     1861 cgattacaag catcattggc ctgggatttt tatatttcat acttccaccc tccctgctcg
     1921 aagttttcta tcttatgata atttttagca cagtcagttt gactactgta tcagggcgat
     1981 atcaggctta ctttacgctg ttagggattc tggcaattac cctaccaagc gagagtcaac
     2041 actttacgaa cacaaaaagt attctcgatt attttatccc gttcgtcacc gccataatgg
     2101 ttgtggaagc ggttcttcaa attaaggaaa ctacacaaag gcatattcat cggcttgaaa
     2161 cgatcaacaa gatcagccgt caaatcatgc tttccttaaa cactgaagag acgatagcac
     2221 ttttggcgac tgccatgcag gatgctgttg aggcagacac ctattttgtc ggcatcgtca
     2281 gggacaatga aattcacctc gatttatttc atgacgatgg agaacatttc aatggtacac
     2341 gcattccgat tgatggcacg ctatcgggct gggtaatcaa aaatcaaaag gaacttttcc
     2401 tcaatgatct cagaaaggat atcaagctgg atggcttgga aagtttggtg agtggaagtg
     2461 aaagaaccag tctttcctgg atgggtgtgc cactcagtgc atcaaacgta gtagggatta
     2521 tggccctcag ttcataccaa cccaacgcat ttgatcgcgc ggatatggag cttctgtcca
     2581 atttggccca acatgtaacg ctggcgttga acaacgcatt tcaacacgaa cgagtggaag
     2641 aacaagctcg tcttgatagc ctgactggcg tatacaatca tggctacttc ttaagaaaac
     2701 ttgccgaaca ggccgacgga tcgtctcatt caggaatacc cctgagcctg atcatgttgg
     2761 atattgatta cttcaaacaa tataacgata cctttgggca tttggttggc gaccaaattc
     2821 taagaacatt gtgcagggca attacgcaac acatcaaaca aactgatgct gtggggcgct
     2881 ggggtggcga ggaatttatt atttcgcttc cgggtgcgag cggcatccag gcgatggaag
     2941 tggcacaacg cattggtcag acgatgtctg gtttgcaagt agaagatatg gatcagaaaa
     3001 aagtgcccgt tccaaccgtt agtcagggaa ttgcggtatt ccccgaggaa gcaagcgaaa
     3061 tttatcgttt gatcgatctt gcagatcaac gtttatatgt ggcgaaggaa caaggcagga
     3121 atcagattga gcccaagtct ttccagcggg aaagaatccc gcactgatac aaaatgaaca
     3181 ggcctttgat atgcgaaatt gaactaattc aggacttcat gaaaagcaga tacagttttt
     3241 tcgacagcag catcatcaat ccaataatgt gtgaccaggc gaaggcgttt tgcattatcg
     3301 gcatctatta gaattcctaa ccctttcagc tgttcaccaa tttgaaaaag atcttgtccg
     3361 gcgttgtctg atatattaag ataaaccatg ttcgtataag gggaattagg atccaggttc
     3421 aggccgttga tattcctcaa gccatctgcc attttcttcg cccgagtgtg atcctctccc
     3481 aaccgcttcg tcatctgctc caacgatatg attccggccg cggcgagaat gcctacttgc
     3541 cgcatcccac cacccaacat ctttcgaagg tgacgagccc gcgcgatgaa tttccgcgtc
     3601 ccaagcacca tcgaaccgac gggcgatgcc aggcccttgc tcaaacaaaa catcacagaa
     3661 tcagctggct ctaccaacgt cgcaaccgat acattttgag ctacagccgc attaaaaata
     3721 cgtgccccat caatatgcaa gagaaggttg ttttcatgag caagttcgcc aatctgacgt
     3781 gtatagtcac cagagagagg cacacctcca caaacatttt gcgtgttttc aaggcaaatc
     3841 aaacgtgtga taggatgatg aacatcctcc gtacgaatcg atgtgaggat atcttccacg
     3901 gcgagcgtgc catccggctg gttttgaatc ggacacggct gaatcccacc tagtgcggac
     3961 atccctccgg cttcattcaa atagatgtga gagcgactcc caacaatgac ttcatctccc
     4021 ctctgacaat gaaccagcaa agcaatgagg ttccccatcg tcccagaagg gacaaataat
     4081 gctgcttcct tgcccagcat ccccgcagct atttcttcaa ggtcattgac agttggatcg
     4141 tccccataga catcgtcacc gacttctgct tctgccatgg cttcgcgcat ttcgggcgtt
     4201 ggttttgtca ctgtatccga acgaagatca atatattcca taatcagctt tcctttcgga
     4261 gggtattcaa tacgctgagg aggtcatctg gtaaatttgc ttcaaagact ctgtgctcgg
     4321 cttccccggg caatacaatt ttcaaccgat acgcatgaag aaaatgacgc ttgatggcga
     4381 tggatatttt ctttctgccg tagattttgt ccccgacgat cggacaccct aaaaaagaac
     4441 agtgcaatcg gatttgatga gtacggcctg ttaaaggatg gaagtcgagc agggtatggt
     4501 ctttgaaatt ttccttcgtt ttatattctg agatcgcctc ccgtccgcgg ctttcgggga
     4561 caatcgccat ttgtttgcga tggcgcgggt cacggccgat aaaggtttcc acacgtccag
     4621 atggcgttgg aggcctgcca tctaccagtg ccagataggt cttttccact tttcgc
//
LOCUS       DASTJP010000888         5187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_16768, whole genome shotgun sequence.
ACCESSION   DASTJP010000888 DASTJP010000000
VERSION     DASTJP010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16768"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFQ23_25815"
     CDS             complement(<1..447)
                     /locus_tag="VFQ23_25815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910090.1"
                     /translation="MTTSRPSSRDISRKLVALIFGANFFGALIIFLYFAVIDPLPVGG
                     VAVRSLETEDILTFGIAGVVIFVGAFGIISWLGRPIQNWLGLIEAGAPASELPSSVAR
                     LVLIWPLIVASLVAATGLLVAIFFSVLYDNPGNFVGIAIGGAVTTTI"
     gene            complement(494..1120)
                     /locus_tag="VFQ23_25820"
     CDS             complement(494..1120)
                     /locus_tag="VFQ23_25820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9910091.1"
                     /translation="MMIRIIIADDHPVVRSGLRALLSSQSDFEIVDEAKDGEEAAKLA
                     IALVPDLILMDLQMPILDGLGSIKLIRDKLPKANILVLTTYGTDSDILPALEAGAMGY
                     LLKDTPPDQLFQAVRNAASGAMALGPRIAEKVTQRLTNSSKNTLSSREIEVLALASQG
                     NPNKEIAKKLFISEATVKSHFVHIFSKLDVADRTAAVTKALKEKIIKI"
     gene            complement(1110..2303)
                     /locus_tag="VFQ23_25825"
     CDS             complement(1110..2303)
                     /locus_tag="VFQ23_25825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9910092.1"
                     /translation="MTKMQKSIKPEAWEQWDWVWHFSAYGLLILNVILVYNNEPRIPN
                     FLFFLVLSVLLGLWYLPFLNISTLRIWDNPKRGALYLVPGWAIWAGLISLTADSLLLI
                     GIFFPLVFSRFPIRWATGITIFQTLGLYFLYIILYPTERWFLILLILLGLLTISTIIG
                     TFISSIIRQSTDRQRLIDELTQSRANLMRVEREAGRLNERQRLARDIHDSLAQHFTSI
                     IMHLAAAKHSNFGTVQTQVQQAEAAAREGLDEIRRIVWDMQPEQIEKASLIEAVEALA
                     ARWSAENRVLVKMNVTGTPRPLTSSAETALLRISQEAMHNINKHAQARNVNVTLSFME
                     DMFVMDIADDGLGFDPSKTSDGFGLKTMRDRIEELSGILTIESERGGGTAIAVSLPLS
                     ENNDD"
     gene            complement(2377..3114)
                     /locus_tag="VFQ23_25830"
     CDS             complement(2377..3114)
                     /locus_tag="VFQ23_25830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9910093.1"
                     /translation="MNTTNNEKSTVWQGVAINERSLVWQRIAIVTLLLVVLVGPNFGP
                     LPHLLRESLSLESSITGVLIWFLVAFMIILAVVYRMAKSTSIREFLITHGLGAPSGIG
                     ANIAGLILGIVWGLLFLSSILQFDPDANLTQINSFRILTALLAAGGALLEDLITRSFI
                     MNQLHKIGSSKWVQVVISALIFALYHTIWGFNPFSFVFSMVYGLLLGGLFLWGKRSLT
                     PVILGHSLVVLIAEPFATMLIFLAPGA"
     gene            complement(3338..3745)
                     /locus_tag="VFQ23_25835"
     CDS             complement(3338..3745)
                     /locus_tag="VFQ23_25835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET9910094.1"
                     /translation="MRKIEVFFYGLFMDEAVLRAKGITSPDLRVAFLPGFQLRIGNRA
                     TLVPIPTERVFGVVASLSHSELEQLYSEPSVQAYKPEAVLAHCLNGEVLAALCFNVVE
                     APSADEHNPEYATKLRALATRLQFPLDYVSSIQ"
     gene            complement(3848..>5187)
                     /locus_tag="VFQ23_25840"
     CDS             complement(3848..>5187)
                     /locus_tag="VFQ23_25840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910095.1"
                     /translation="ALEAVSEDPNAFEHLEGLVNKSLVVTEEHKSEMRYFLLETIRQY
                     AREKLFEAKQAAAARDRHFIHYKNRTNDLWGATYFATEEETLRLMSIQVEVENLRAAF
                     EWGLQNHVEDTLELIASMAMSMSMVGGQLEGITMLKTAMEKLRALPPVEGEALRLREE
                     IYIHGCLSLGALLQGTNEIMLSRSILQEAIAISRELGDKHLLGMSLEMYANTSAMIKA
                     DDTPAAAKEGLEIFRELNYNPGMWMAYSNLARWESMGRNFQESEKYVELAQASMKAGT
                     ISVQFGFLNMGMGMSARFQGRFERAQQYFEEGLRIFKHIGHKGLMALMTSEIAHTQRA
                     MGNFSDSKQTYRETIKIFQDYGNRPAVAHQLECFAMIAVAEEEPQHAAKLFGAAEAIR
                     EVTGHKRTDEEEAEEGQFMSRLRAMLSEAEFNTFWAEGKSLTMEQAIQLALNV"
BASE COUNT         1329 a         1298 c         1222 g         1338 t
ORIGIN      
        1 aatggttgtc gtgacagcac cgcctattgc tatgccgaca aagttgccag gattatcgta
       61 caaaacagag aaaaatatag ccaccaacag cccggtcgcg gccacgagtg aagcgacgat
      121 gagaggccaa atcagtacca aacgggcaac actcgacggc aattctgaag cgggcgcgcc
      181 agcctcaatt aaccccagcc aattctggat tggtcgccct agccagctga ttatcccaaa
      241 agctcctaca aatatcacga caccggcgat cccgaaggtc aggatgtctt cggtttcgag
      301 tgagcgaacc gccactccac ccacaggaag cggatcgata accgcgaaat aaagaaagat
      361 gatcagcgct ccaaagaagt tcgctccaaa gattaacgcc acaagcttgc gagaaatgtc
      421 tcgtgaagat ggccgggaag tagtcatggt agcgtcctgt tctctgtaaa gggtgtggat
      481 ttcacaaaaa ccatcatatt ttaataatct tctcttttaa tgctttggtt actgcggctg
      541 tgcggtcagc tacgtccaat ttagaaaaga tatggacgaa atgagattta acagttgctt
      601 cagaaatgaa taatttcttc gcaatttcct tgttaggatt tccctgcgat gcaagtgcta
      661 acacttcaat ttcacggctg ctcaatgtgt ttttcgaaga atttgtcaaa cgctgcgtca
      721 ctttttctgc tatgcgcggc cctagcgcca tcgcgccact ggctgcattg cgcaccgctt
      781 gaaatagttg gtccggagga gtgtctttta gtaaataacc cattgctcca gcttcgagag
      841 ctggcaatat gtccgaatct gttccatacg tagtaagcac caaaatattt gcctttggca
      901 acttgtctcg aatcagtttt attgacccca gaccatctaa gatgggcatt tgcagatcca
      961 tgagaattaa atccgggacg agcgcgattg ccaatttggc agcctcttcg ccatccttag
     1021 cctcgtctac tatttcaaaa tccgattgcg acgatagcaa tgcacgcaac ccactgcgaa
     1081 ccactgggtg gtcatcagca attattatcc taatcatcat tattttccga gagaggaaga
     1141 gatacagcaa tcgcagttcc tccacctcgc tcactttcaa tggtcaatat tccacttaat
     1201 tcttctattc tatctcgcat cgttttcagt ccgaaaccat cgctggtttt cgatgggtca
     1261 aaacccagtc cgtcatcggc aatatccatc acaaacatat cttccataaa cgaaagggtg
     1321 acattgacgt ttctagcttg cgcgtgctta tttatattgt gcatggcttc ctgtgaaatg
     1381 cggagtagtg ccgtttcggc ggaggaggtg agggggcgcg gggtacctgt cacgttcatt
     1441 tttaccagca ccctgttttc tgcagaccag cgtgccgcta atgcttcgac cgcttcgatc
     1501 agagaagcct tttcgatttg ttccggctgc atgtcccaaa caattctgcg aatctcgtca
     1561 agcccttccc gtgcggccgc ttccgcttgt tgaacttgcg tttgaactgt cccaaaattg
     1621 ctgtgttttg ccgcagccag atgcatgatg atggaggtga aatgttgggc gagtgaatcg
     1681 tgaatgtcac gtgcgaggcg ctgccgttca ttcagacgac ctgcctcccg ttccactctc
     1741 attaaatttg ctcgggactg tgttaattca tcaatgaggc gttggcgatc tgtgctttgt
     1801 cttatgatgg atgaaataaa agtacctatg atcgtcgaaa tcgtcagcaa gcccaaaagg
     1861 atcagtagga tgagaaacca gcgttccgtg ggatatagaa tgatatagag gaaataaagg
     1921 cccagggttt gaaaaattgt tattccagtt gcccagcgta tggggaagcg gctaaatacc
     1981 aatggaaaga atatccctat caacaacagc gaatcggcgg tcagtgagat caaaccagcc
     2041 cagatcgccc atccgggaac cagataaagc gcgccgcgtt ttgggttatc ccagatacgc
     2101 aaggttgaaa tgtttaaaaa tgggaggtac cacaagccca ataacacaga aagaaccaga
     2161 aagaacaaga agtttgggat tcttggttca ttattgtata caagtatgac gttcaagata
     2221 agcaggccgt aggcagaaaa gtgccatacc caatcccatt gttcccacgc ctcgggtttt
     2281 atgctctttt gcattttcgt catcgcggca ttataccctg cgatatctgg caaaaaaaca
     2341 acgccagata tcgcaggata atatctaaac gtacttctag gcacctggtg caaggaatat
     2401 tagcattgtg gcaaaaggtt ccgcaatcag gacaaccagg ctgtgtccga gaatgaccgg
     2461 agtaagactg cgtttgcccc ataagaacaa tccgcccagc aataatccat agaccatact
     2521 aaagacaaat gaaaagggat tgaagcccca gatcgtatga tacagtgcaa agatcaaggc
     2581 ggagataact acctgcaccc atttggaaga gccaatttta tgcaactgat tcatgatgaa
     2641 gctgcgtgta attagatctt ccagcagtgc gccgcctgct gccaataatg cggtcagaat
     2701 tcggaagcta ttgatctgtg tcaggttggc gtccgggtca aattgaagga tgctggacag
     2761 gaatagcagc ccccatacga ttcccaggat taaccctgcg atgttcgcgc cgattccaga
     2821 aggtgcgcct agcccatggg tgataagaaa ttctcgaata gacgtgctct ttgccattcg
     2881 atatacgact gccaggatga tcataaaggc gaccaggaac caaatgagaa cgccagtaat
     2941 ggagctttcc agagaaaggc tttcccgaag caggtgcggc aacgggccga agttgggccc
     3001 aaccagcacc accaatagaa gcgtaacaat tgcgatcctt tgccatacca ggcttctttc
     3061 attaattgcg accccctgcc agacagtact tttttcgtta tttgttgtgt tcattttctt
     3121 cctctctctt tgataatttg gttttgatga acgaagtgta gaataaattt gttcgggtgt
     3181 catcaagaga gagatggatt tagtaatcaa tcaaaagttt gattaattag agaagtgcct
     3241 ttgataagat ccttccttca tagcgtcgat cgagaaaatt gatatgaatt acattaagat
     3301 ggaacatatt ggtaggctac tgtgaaagtt acagagctca ttggatggat gaaacataat
     3361 cgagcggaaa ctgaagccgt gttgcgagtg cgcgtagctt ggtagcatac tcaggattgt
     3421 gttcatcggc tgacggtgct tctactacgt tgaagcagag cgccgccagg acctcaccat
     3481 tgaggcagtg tgccaggacc gcctccggct tataagcttg tacactgggt tcggagtaaa
     3541 gttgttccag ctcactgtgg gaaagggagg caacaacgcc aaacacgcgc tccgtgggaa
     3601 tcgggacaag tgtggcgcga ttgccgatcc gcaattgaaa tccgggcaaa aatgccacac
     3661 gcaggtctgg tgaagtgatg cctttggccc gcagcacggc ttcatccata aaaaggccat
     3721 agaagaaaac ctcgattttt ctcatgagtt gcctcctaaa cttggcgttg tggcgagcag
     3781 gcctccggcg aacccacctg tcctgggagc gtagcggatg gggactttgt tggccctgta
     3841 ttttttgcta cacgttcaat gccaactgga tcgcctgctc catcgtcagg gatttgccct
     3901 ccgcccagaa cgtattgaac tccgcttcag agagcatggc gcggaggcga ctcatgaatt
     3961 gaccctcttc cgcttcttcc tcgtccgttc gtttatgacc tgtcacctca cggattgcct
     4021 ctgccgcgcc gaataatttt gcggcatgtt ggggttcttc ctccgcaact gcgatcattg
     4081 caaaacattc cagttgatgc gccacagccg ggcgattccc gtaatcttga aaaatcttga
     4141 tggtttccct gtaggtttgc ttggaatcac tgaaattacc catggcacgt tgtgtgtgcg
     4201 caatctcgga agtcattaat gccatcaagc ctttatgacc gatgtgcttg aatatcctga
     4261 gaccttcctc aaagtactgc tgggcacgct cgaagcgacc ctgaaaccgc gcgctcatcc
     4321 ccatccccat attgagaaaa ccaaattgaa cagatatggt tcctgctttc atgctagcct
     4381 gagccaactc cacatacttt tcactctctt gaaaattgcg acccatcgat tcccagcgtg
     4441 ccagattgct ataagccatc cacatcccgg ggttgtaatt gagctcccta aaaatttcaa
     4501 gcccctcttt tgcggcggca ggggtatcat cggctttgat catggcggat gtgttcgcgt
     4561 acatttccaa actcatgcct aacaggtgtt tgtcgccaag ttcgcgagaa atggcaatgg
     4621 cttcctgcag gatactgcgc gaaagcatga tttcattcgt gccttgcaag agcgctccca
     4681 ggctaagaca cccatgaata taaatctcct ctcgcaggcg aagcgcctca ccttcaacgg
     4741 gcggcaaggc gcgtaacttc tccatggctg ttttgagcat ggtgattcct tccagctgtc
     4801 ctcccaccat ggacatactc atcgccatac tcgcgatgag ttccaatgta tcttcaacat
     4861 ggttttgcaa accccattca aatgccgccc gcaggttttc cacttccact tgaatggaca
     4921 tcaatcgcag cgtttcttct tctgttgcga agtaagtggc gccccataag tcgttcgtcc
     4981 tatttttata gtggatgaag tgccgatcac gcgccgccgc agcttgcttc gcttcgaaga
     5041 gcttttcgcg cgcgtattgc cggatcgtct ctagcaggaa atatcgcatc tcgcttttgt
     5101 gttcttctgt gacgaccaac gatttattta ccagcccttc gagatgctca aatgcattcg
     5161 ggtcttcgga cacggcttca agcgcct
//
LOCUS       DASTJP010000889         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_720377, whole genome shotgun sequence.
ACCESSION   DASTJP010000889 DASTJP010000000
VERSION     DASTJP010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720377"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFQ23_25845"
     CDS             <1..282
                     /locus_tag="VFQ23_25845"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9910096.1"
                     /translation="LEGEVIGTLHLIFLPSISYQGGLRAQVESVRIDTRHQSQGFGSQ
                     MLKWAIGRVKVRGAHIVQLTTHQSRADAHRFYERLGFKGTHLGMKLSLK"
     gene            complement(344..437)
                     /locus_tag="VFQ23_25850"
     tRNA            complement(344..437)
                     /locus_tag="VFQ23_25850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(401..403),aa:Ser,seq:gct)
     gene            complement(478..565)
                     /locus_tag="VFQ23_25855"
     tRNA            complement(478..565)
                     /locus_tag="VFQ23_25855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(529..531),aa:Ser,seq:tga)
     gene            844..1200
                     /locus_tag="VFQ23_25860"
     CDS             844..1200
                     /locus_tag="VFQ23_25860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910097.1"
                     /translation="MATYSSKQEKRQVFGNYVPVLVSVLLVGVLVVLAPLNASFSSSL
                     NGIFGTWTSADGVYTNAEVISFNADQTYWDVNCTHGWNADAACDSILSRVQSCTISVT
                     SAYCASYEDYMQGLFK"
     gene            complement(1317..1388)
                     /locus_tag="VFQ23_25865"
     tRNA            complement(1317..1388)
                     /locus_tag="VFQ23_25865"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1354..1356),aa:Lys,seq:ctt)
     gene            1569..2843
                     /locus_tag="VFQ23_25870"
     CDS             1569..2843
                     /locus_tag="VFQ23_25870"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HET9910098.1"
                     /translation="MNYSEPASQPTPEQSEEFVSARARRRRAQRRAYFPTDEEGRAAL
                     FTHLARRAYPSYELFVFALVSGAILGLGYFINSQALLIFGILVAPLMTPWIGMSLAIV
                     AGSARFFLQTVTALFVSCLLIFLSGILAGFASRTFGPLTFNEAFTHSRLWWPDLVVIT
                     IGSIILTISFVRSEERPYLPSALLTYGLFLPLCAGGFGLGSGVGEIWPQGLYVFLVHL
                     ALATFFGILTLFFLRFYPTSAGGIAFTGLTFITLIVLVIFLTGFNRWIMIQAGLATLE
                     PTPVSQVSLTPAQLPTVTVSPRPQQTDALGATIPTATRGVTRTSTPTIPPTETSTSTI
                     TAEPTPIIAIIRAAEGGGAFIRERPGGVVVATLGNGSTVTIFPNDFQDVNNVIWVHVF
                     AIVNDVRVEGWMIQSLLQTATPIANWQPSPTP"
     gene            2894..>3104
                     /locus_tag="VFQ23_25875"
     CDS             2894..>3104
                     /locus_tag="VFQ23_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HET9910099.1"
                     /translation="MPIHFGTDGWRAVISDTFTFDNLRIVAQAIADAVGSDHWDTSGK
                     SEQTPGPKKIIVGFDTRFLSDRYAGE"
BASE COUNT          743 a          779 c          724 g          858 t
ORIGIN      
        1 ttggaagggg aggtcatcgg aacgctccat ctcatatttc ttccctcgat aagttatcag
       61 ggtggcttgc gtgcccaggt cgaatcagtg cgtatagaca ctcgccatca aagtcaggga
      121 tttggaagcc aaatgctgaa atgggcgata ggacgtgtaa aagttcgcgg tgctcacatc
      181 gttcaactga caactcacca atcccgcgca gatgcccatc gattttatga acgattgggt
      241 tttaaaggaa cacatctggg gatgaagttg agtctcaaat agtattcata atttctaccg
      301 gttaaaaaca aaatgcgctt ctacttgagc gcattttttc ttctggcggg gaggacagga
      361 ttcgaacctg cgactgagga tttcgcccca gtaaccgctt agcaggcggc cccattcgac
      421 cactctggca cctccccaat atgtttacga ttgcgaagta tcaagtcgtc aatcgtctgg
      481 cggagggagt gggattcgaa cccacgttgg tgtgacccaa acatgttttc aagacatgcg
      541 ccttcagccg ctcggccatc cctccacaag gtcccgacgt tttatcaggg gcgcaaagat
      601 tttaccatgt ttttatattt gaacaatttg cctgcatttt atgtgaattt ctcaatttat
      661 ccaaatctaa gaaatgacct tgaatcttgc atgaatgcaa attgcttcct gtaaatcgat
      721 tactgttttg ttaggcggac gaaaaattac tgatctgcaa acggaatcga ccccttgcta
      781 gtttacatgg acaagttaga tcagtatgat gaattcatca taaccaaata ggagatgttc
      841 atcatggcaa cgtattcttc aaaacaagaa aaaagacaag tcttcggaaa ttatgttccg
      901 gttctggttt cagttttgct cgttggagtg ctggtggttc ttgccccgct aaatgccagt
      961 ttctccagtt ctctcaatgg catctttgga acatggacca gtgcggatgg cgtttatact
     1021 aacgcggaag tgatctcgtt caatgcggac caaacgtatt gggatgtgaa ctgcactcac
     1081 ggctggaacg cagatgcggc gtgcgatagc atcctttcaa gagttcagtc ctgcacgatt
     1141 agcgtcacct ccgcatattg cgcgagttat gaggactata tgcaagggct ctttaaataa
     1201 acagtaacta accaacttca ccttcatcac ggatatttgc gcaggctgca gatatccgtg
     1261 atttgttatt ttaaagatac agagtttgac agctagctgt caaactctgt agtaggtgag
     1321 tccgctcggg gtcgaaccga gaacctaacg cttaagaggc gcttgctctg ccattgagct
     1381 acggacccag ttgttgaaga ttcgcacggc gaacctgtta tgcggacaga aattataccc
     1441 gaaggggaat acgtgtcaat aagcgcgact atctttcagt tttcttgaga aagtaatatt
     1501 aaagacgatc aatctgtcct ccatcctctc ctttatccct gtataattga atcatcagtt
     1561 tacattaaat gaattattca gaacctgcct cccaacctac tcctgaacaa tcggaagaat
     1621 ttgtttctgc gcgtgcccga aggcgacgtg cccaacgacg tgcctatttc ccaacggatg
     1681 aagaagggcg tgctgcacta ttcacgcatc ttgcgcgccg cgcgtatcca tcgtatgaac
     1741 ttttcgtgtt tgcacttgtc agcggagcga tcctggggct gggttatttt attaactcac
     1801 aggcactgct catctttggc atcctggttg ctcccttgat gactccctgg attgggatgt
     1861 cgctggcaat tgttgcaggc tctgctcgtt tctttcttca aacggttacc gcgctctttg
     1921 ttagttgcct gctgatcttc ctgagtggga tcctggctgg gtttgcctcg cgcacgtttg
     1981 gccctcttac cttcaatgaa gcgtttacac atagccgcct gtggtggccc gatctcgtcg
     2041 ttattaccat cggttcaatc attctgacca tttccttcgt gcgctcagaa gagcgaccgt
     2101 atctgccaag cgccctgctg acgtacgggc tcttccttcc actctgcgcg ggaggctttg
     2161 gattgggcag cggcgtaggt gaaatatggc cgcaagggtt gtatgtattc cttgttcatc
     2221 ttgcattggc gactttcttt ggtatcctta cccttttctt tttacggttc tatcccactt
     2281 ccgcgggtgg aatcgctttt accggactaa catttattac gttgatcgtc ctggtgattt
     2341 tcttaactgg cttcaatcga tggatcatga ttcaggcagg tctcgccacg cttgaaccga
     2401 ctcctgtttc tcaagtatcg ttaactcccg cacagcttcc cactgttaca gtatcgccgc
     2461 gaccgcagca gaccgatgcg cttggagcca ccataccgac tgccacacgt ggagtaacgc
     2521 ggacctcgac tcccacaata ccgcctactg aaacatccac atctacaatc actgccgaac
     2581 caactcccat catcgcgata attcgggctg ctgaaggtgg tggtgctttc atccgtgaaa
     2641 gacccggagg cgtggtagtt gccacactgg gaaatggctc gacggttacg atcttcccaa
     2701 atgactttca ggacgtaaac aatgtgatct gggtacatgt ctttgcaatt gtcaacgatg
     2761 tgcgcgtcga ggggtggatg atccagtccc tattacaaac tgctacaccc atagccaatt
     2821 ggcaaccttc acccacacca taactgcctc agcggcggtt tgaccatttt agaattcata
     2881 ataaggaaac cttatgccca ttcactttgg aaccgacggc tggagagccg tcatctcaga
     2941 tacctttaca ttcgataatc tacgcattgt cgcgcaagcc attgcagacg cagtggggtc
     3001 cgatcattgg gacacgtctg gcaaaagcga acaaacaccg ggaccgaaaa agatcatcgt
     3061 cggttttgat acgcgctttt tatcagaccg atacgcggga gaag
//
LOCUS       DASTJP010000890         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_793207, whole genome shotgun sequence.
ACCESSION   DASTJP010000890 DASTJP010000000
VERSION     DASTJP010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793207"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFQ23_25880"
     CDS             complement(<1..771)
                     /locus_tag="VFQ23_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9910100.1"
                     /translation="MKLHEYQSKTIFAKYGIPIPKGRVAATAEEAKHIAEELGGRVVI
                     KSQVLVGGRGKAGGVRVAKSADEAEQFAQQILTMKIKDLPVRKVLVDEAASIEHEIYF
                     AITNDRAAKKPVMIASAAGGIDIEEVASATPEKIIKVHIDPLLGLRDYQARDIAASID
                     LPRELWRDFGKIAAGLWQAYLETDASLAEINPLVITKDKRLVALDGKMMIDDNALFRH
                     SKLAEIRDIDEEVPAETEARRYGLSFIKLDGNIGCMVNG"
     gene            948..2696
                     /locus_tag="VFQ23_25885"
     CDS             948..2696
                     /locus_tag="VFQ23_25885"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9910101.1"
                     /translation="MLMLLTLAGLSLLIPRIIQTVIDNGLLQRDASYLIRAALLLFGL
                     GIASAILNLGNRYGSEWVAARVGYDLRNRMYDHIQHLPFTYHDHVQSGQLISRCIEDV
                     RAIERFAGGAVADLVRFVLLTVGILYIMVSANPLLAVIALLPMLPLALMTTIFGTRVG
                     GLFFAVDNAVGEVSNRLQENVVGVQVVRAFAREEYEIRRFDTANKEVFKTWIHVIDEW
                     AKIMPTTNWLTAISIILILWFGGQMVMDGTLTIGAIVAFNAYILMLAEPAQQLTGLVN
                     AGGEAAAGAQRVFEVLDTQPAIESAPYAVKIEKLRGEVEFRNVGLKYQDEKTRSLKGI
                     NLRVEPNRIVALIGPTGSGKTSFVNLIPRFYDVTEGEVLVDDVDVRELDLVSLRRQIG
                     IVLQTSLLFSDSIKANIAYGRPDATMEEILVAAKAAQAHEFIEGFPNGYDTIVGERGV
                     TLSGGQRQRVAIARALLMDPRILILDDSTSSVDTQTEKLIQAALDTLMEGRTTFVIAH
                     RLSTVRRADMILVMDKGEIVERGTHKDLLEQGGLYKEIHDLQLIDHAKFAEEMEELQD
                     DYRRAERRHEAEGGVA"
     gene            2851..>3089
                     /locus_tag="VFQ23_25890"
     CDS             2851..>3089
                     /locus_tag="VFQ23_25890"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET9910102.1"
                     /translation="MPYAIYILRCSDGTYYTGLTKDLDGRISEHQAGKISGSYTFSRR
                     PVKLMWSIVTESYKEAFQWEHQIKGWSRAKKEALIR"
BASE COUNT          747 a          751 c          787 g          804 t
ORIGIN      
        1 gccgttgacc atgcatccga tattgccatc cagcttgatg aacgagagcc cataccggcg
       61 agcttccgtc tcggctggaa cttcctcatc aatatcgcgt atttctgcga gtttggaatg
      121 cctgaataac gcattgtcgt cgatcatcat tttgccatcc agcgcgacaa gacgtttatc
      181 tttggtaata accaatggat tgatctctgc caaagatgca tcagtttcga ggtaagcctg
      241 ccataggccc gcggcgatct ttccgaagtc gcgccagagt tcgcgcggca aatcaattga
      301 cgcagcaata tcgcgcgcct gatagtcacg gagtcctaac aacgggtcaa tatgcacctt
      361 gatgattttt tccggagttg ccgaagcaac ctcttcaata tcgataccgc ctgcagccga
      421 tgcgatcatc accggcttct ttgccgctcg atcatttgtg atggcgaaat aaatttcgtg
      481 ctcaatcgat gcggcttcat cgaccagaac tttacgaacc ggcagatctt tgatcttcat
      541 ggtaaggatt tgttgagcga actgttctgc ttcgtctgca ctctttgcga ctctcacacc
      601 cccggctttc cctcgtcccc ctacaagtac ctgcgattta ataacaacgc ggccacccag
      661 ttcttcagcg atatgtttcg cttcttctgc ggtagccgct acccgtccct ttgggatggg
      721 aattccgtac tttgcaaata tggtttttga ttgatattcg tggagtttca ttttaccctt
      781 tggaaacctg caaatttatg aaacacgggc attataccat ttgaaattat ctctacaaat
      841 ctcgaagccc tgaaacattt tatggtatcc tcttaagccc gtatatgaag cacttactcc
      901 gaatacagat ttttttaaaa ccctatcgtt ggcagatcct ggccacgatg ttaatgctcc
      961 ttacgctggc cggactcagc ctgctcatcc cgcgcattat tcagaccgtg attgataatg
     1021 gactgctgca aagggatgcg agttatttga tccgcgctgc acttttgctg ttcgggcttg
     1081 gaattgcctc cgcaattttg aatctgggta accgctatgg ctcggagtgg gtggccgcgc
     1141 gcgtaggcta tgaccttcgc aaccgcatgt atgatcacat ccagcatttg ccatttacgt
     1201 atcacgacca cgtccaaagc gggcaactca tctcgcgctg tatcgaagac gtccgtgcga
     1261 tagagcgttt tgcaggcggc gcggtcgctg acctggttcg ttttgtcctg ttaactgtgg
     1321 ggatccttta cattatggtc tcagccaatc cgctgctggc ggtgattgcc ttgctgccga
     1381 tgctgcctct ggctttgatg acaactatct ttggtacacg agtgggtgga ttattctttg
     1441 ccgtggataa tgccgtgggg gaagtctcca accgcttgca ggaaaatgtg gtcggcgtgc
     1501 aggtcgtccg tgcatttgcc cgtgaagagt atgagattag acgattcgat acggcaaaca
     1561 aggaagtctt caaaacatgg attcatgtca tcgatgagtg ggcaaagatc atgcccacca
     1621 caaactggct gacagccatc agcatcatct tgatcctctg gtttggcggg caaatggtaa
     1681 tggacgggac tctcacgatt ggcgcgatcg tggctttcaa cgcctacatt cttatgctgg
     1741 cagaaccagc acaacaattg acaggtctcg tcaatgcggg tggcgaggca gccgcgggag
     1801 cgcagcgtgt gtttgaggtg ctggatacac agcccgccat tgaatctgcc ccttatgcag
     1861 taaaaataga gaaattgcgc ggcgaagttg aatttcgaaa tgtggggctg aaatatcagg
     1921 atgaaaaaac ccgttctcta aaagggatca acttgcgggt tgagccgaat cgcatcgtcg
     1981 ccctgatcgg cccgaccggt tcaggaaaaa cgtcgtttgt gaatctcatc ccgcgttttt
     2041 atgatgtgac cgaaggcgaa gtgttggtcg atgacgtcga cgtccgcgag ttggacctgg
     2101 tttcgttgag aagacagatc ggcattgttc tacagacgtc tctattgttc tcagattcaa
     2161 tcaaagcgaa tattgcctat ggacgcccgg atgcaacaat ggaagaaatt ctggttgccg
     2221 cgaaagctgc tcaggcgcat gaattcattg agggttttcc caacggatat gacacaatcg
     2281 tcggcgagcg cggtgtgact ttatctggtg gacagcgtca gcgcgtggcg attgcccgcg
     2341 ccctgctgat ggacccgcgc atattgatcc tggatgactc gacttcttct gtagacacac
     2401 aaactgaaaa actgattcaa gctgccctgg ataccttgat ggaaggacgg acgacctttg
     2461 tcattgcaca tcgcctctct actgtgcgcc gcgcggatat gattttggtg atggacaaag
     2521 gggaaatcgt ggaacgcggt acgcataagg atttactaga gcaaggtgga ttgtataaag
     2581 aaatccacga tttgcagtta atcgaccatg ccaaatttgc agaggagatg gaagagttac
     2641 aggatgacta tcgccgcgcg gagcggagac acgaagcgga aggcggagta gcataattat
     2701 gtacactcgc gctgagcgga acgatgacag tttgtacaac gagtgtagac gaaggtcaga
     2761 tttcataaac tctttcattt atccgcaatg ggcgcttcgt ctgcgggcgg aagatcacca
     2821 ccctccgctc agcgcgaaag agagttccaa atgccatatg caatttatat tctccggtgc
     2881 tccgatggaa cttattacac tggactcact aaagatttgg atggccgaat ttcagaacat
     2941 caagctggca agatatctgg atcatacaca tttagccgtc gtccagtaaa gctgatgtgg
     3001 agcatagtaa ctgaatctta taaagaagca tttcaatggg agcatcaaat taaaggctgg
     3061 agccgtgcca agaaagaggc gctaattcg
//
LOCUS       DASTJP010000891         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_616082, whole genome shotgun sequence.
ACCESSION   DASTJP010000891 DASTJP010000000
VERSION     DASTJP010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616082"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VFQ23_25895"
     CDS             complement(<1..1103)
                     /locus_tag="VFQ23_25895"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9910103.1"
                     /translation="MKPPASPSEKIITWTTSQVVLATVFVVCVFLIFWLLYRLSFLIF
                     ILFVAIVIGTALRPVVEWLQRRGISRMAGVIIIYILLAGLMIGFLALIFPLVAEQATQ
                     LSQNLPDYYGEIRRALVTSNNRLLQNIAFRIPEDLSLLVVAPPDSSEALLTQVTQTVF
                     YANVAVKGILSILAVFLLAYYWTQESTQVVRSIIRLVPLHRRKETQEFLQLAERKIGG
                     YIRGQGILCLVVGIAAFVFYSLIGLPYTLVLAIFAGLMEMIPVFGPALGAVPALLAAL
                     STDPSKAVWVLVVTGLIQMMENLWLVPRIMKNSMGVNPIIILLSLVAFGTVFGFAGAV
                     LALPLAAIIQLILDRILVSAEESNDSSIDKEAHV"
     gene            complement(1100..1474)
                     /locus_tag="VFQ23_25900"
     CDS             complement(1100..1474)
                     /locus_tag="VFQ23_25900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910104.1"
                     /translation="MPKTKKWIVSLVCAGLLLAACSGNATDIPAVATQIIPALENVLP
                     PDVALNIRNQVSEILGVPVESIQLETVEQTEWPDSCLGLGGPDESCAQAVTPGWLLVF
                     SANGQQYSFRADQTATVIRQEP"
     gene            complement(1496..1648)
                     /locus_tag="VFQ23_25905"
     CDS             complement(1496..1648)
                     /locus_tag="VFQ23_25905"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HET9910105.1"
                     /translation="MSTILLIVLVLLLLGALPVWPYSRSWGYGGSGLIGFLLLLVILG
                     ALFNWF"
     gene            complement(1729..1938)
                     /locus_tag="VFQ23_25910"
     CDS             complement(1729..1938)
                     /locus_tag="VFQ23_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET9910106.1"
                     /translation="MNRNILEGNWKQIRGTIREKWGELTDDELDQIAGKRDKLAGVLQ
                     ERYGYTQVEAERQIDDFLESWEDAD"
     gene            complement(2240..2515)
                     /locus_tag="VFQ23_25915"
     CDS             complement(2240..2515)
                     /locus_tag="VFQ23_25915"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9910107.1"
                     /translation="MTLTGLIILLLIAGIVGAIGQSLSGYSFGGCLVSIIVGFVGAYI
                     GVWLAAQLGLPEVFAINIEGESFPIVWGVIGSAILSLILGLFTRRGF"
     gene            complement(2769..>3422)
                     /locus_tag="VFQ23_25920"
     CDS             complement(2769..>3422)
                     /locus_tag="VFQ23_25920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9910108.1"
                     /translation="VDETLSMTKKHIRVLIVDDHPGVRAGIKNLLSAAKDIEIVGEGE
                     NGAQAIELVATKNPDILLLDMELPDQRGDIVMRTIRDMEPDVKVLAVSSYSDRDYILG
                     MMENGAAGYITKDEAPTMLLEAIRSIVHKNQNWFSPQAIMISTPTPLEQQTLTKREIQ
                     ILEQLVRDRSADEIAASLGISKTQVESFLKLLMKKYETDSLLTLKNIAQRVLSRRNS"
BASE COUNT          924 a          889 c          731 g          878 t
ORIGIN      
        1 acatgagctt ccttgtcaat tgatgagtcg ttggattcct ctgcggagac taagatccgg
       61 tccagaatca gttgaatgat cgccgctaac ggtaacgcca gcaccgcgcc ggcaaagcca
      121 aagaccgttc cgaatgccac cagggataat aagataataa tgggatttac tcccatggaa
      181 ttcttcataa tacgagggac cagccataaa ttttccatca tctggatcaa gccagtaacc
      241 accaaaaccc agaccgcttt gctgggatct gtagataaag ctgccagcaa tgctggaacc
      301 gcacccagtg caggaccaaa cactggaatc atctccatca ggccagcaaa aattgccagg
      361 accaatgtgt agggaagacc gatcaatgaa taaaagacaa aagccgctat accaacgacc
      421 aaacaaagaa ttccctgccc gcgaatataa ccaccgatct ttcgttctgc caattgcagg
      481 aattcctgcg tctcttttcg gcgatgcagg ggcaccaacc gaattatgga tcgcaccacc
      541 tgggtgcttt cctgagtcca gtaataggca aggaggaaaa ccgctaaaat gctcaatatc
      601 cccttcaccg ccacatttgc ataaaagacg gtttgagtta cttgagtgag caatgcttca
      661 cttgaatcgg gtggcgcaac caccagaagt gataaatcct cgggaattcg aaacgcgata
      721 ttttgcaaca aacgattgtt ggaggttact aatgcccgac ggatctcgcc ataataatca
      781 ggcaaattct gtgaaagctg tgtggcctgt tccgcgacca acggaaagat cagagccagg
      841 aacccaatca tcaggccggc aagcaggata taaataataa taacccctgc catgcgagat
      901 ataccacgcc gctgcagcca ttcgactaca gggcggagcg ccgttccgat cacaatcgcc
      961 acaaacagaa taaagatcag aaagctcagg cggtataaca gccagaaaat taaaaagacg
     1021 cagaccacaa atactgttgc aagaacaacc tgacttgtgg tccacgtgat tattttttca
     1081 gatggagatg ccggtggctt catggctcct ggcgaatcac cgtcgcggtc tgatcggcac
     1141 ggaagctata ttgctgtccg ttggcagaga acactaacaa ccatccgggg gtaaccgcct
     1201 gtgcacagga ttcatcgggg ccaccgaggc ccaagcagct atctggccat tccgtctgtt
     1261 cgactgtttc gagttggatg ctctctacag gtactcccag gatttcactg acctggttcc
     1321 gaatgttcag agccacatcg ggcggtaaaa cattctccaa agcagggata atctgggtcg
     1381 caacagcagg gatatcggta gcattgccac tacaagctgc aagaagaagg cctgcacaca
     1441 cgagagacac aatccatttc tttgttttcg gcatctttcg tttcatcctt tccagttaga
     1501 accaattaaa taaggcaccc aggatcacca ggagcaaaag gaacccgatg agtccgctgc
     1561 caccatatcc ccaactgcgg ctataaggcc atactggcag ggcacccagt agtaacagaa
     1621 ccaacacgat caataaaatt gtactcattt ttattctcct ttttgtttag aaccagaggg
     1681 gaaggatccc ctctggtaag ttctttgaac gtctatgctg acctgaaatt agtcagcgtc
     1741 ttcccagctc tcgaggaaat cgtcgatctg tcgttcggct tccacctggg tatagccgta
     1801 gcgttcctgc agcacaccgg ctaacttatc gcgtttaccg gcaatctgat cgagttcatc
     1861 gtcagtcagc tcgccccatt tttcacggat ggtgccgcga atttgcttcc aattaccttc
     1921 taagatattt cgattcattt ttttctcctt ttttgtataa cgttctaatc tatacgcttc
     1981 caagttccgt tcttcttctc gtaactcttt ttgacagcac tccaggcgac cctatgcgag
     2041 acttcctctc tggaagcgcc tttcctgcga tcctccgcat ttttatattc ctcccacgca
     2101 ctgttatagg ctgccatata aacttcctga gcgtgcttcg gaagattatc tcgaaccgat
     2161 tctggtaact ctttcacact atcgtaaggc atcgaacgat ccattccatc cgttgataat
     2221 cagagatccc tgatttcaac taaaaacctc tccttgtaaa caatccaagg atgagcgaca
     2281 ggatggcgga accgatcacg ccccatacaa tcggaaacga ctctccttcg atgttgatcg
     2341 cgaaaacctc gggcagacct agttgagcgg ccagccatac gccgatataa gctccgacaa
     2401 atccgacgat gatcgaaacc aaacaccctc caaacgaata gccagagaga gattggccga
     2461 tggcgccgac aattccagcg atgagcagta atatgattaa tcctgtgagt gtcatttcta
     2521 cctccaaagc attgatgctt tattgaagtc tttgttgtga tttgaacgta cccttccaaa
     2581 tcattggaat gacactacta taaaagttag gctttcttat atcaatgggg gatttaccta
     2641 ctaattcggg agacacccta ttaatttcat tgatgtctta tagaactaaa acaaaaaatt
     2701 caggatatac atcctgaatc tctgattcga tttgaacgta agcgactgtc ttttttcgcg
     2761 cttacaactt aagaattccg ccgtgagagg acgcgctggg caatattttt taacgtaaga
     2821 agtgagtcgg tctcatactt cttcatcagc aatttcagga agctctccac ctgggttttg
     2881 ctgatcccca gagaagcagc gatctcatct gcagagcgat cacgcaccaa ttgttcaagg
     2941 atttgaattt cacgtttggt tagtgtttgt tgttccaatg gagtgggtgt gctaatcatg
     3001 atggcttgag ggctaaacca gttttggttc ttatgcacaa tactgcggat ggcttccagc
     3061 agcatggtgg gcgcttcatc ttttgtaata taccccgccg cgccgttttc catcatgccg
     3121 agaatgtaat ctcggtcact ataggaacta accgccagca cttttacatc tggttccata
     3181 tcgcgaattg tccgcatgac aatatcccct ctctggtctg gcaattccat atccagcagc
     3241 aagatatctg ggtttttcgt tgcaaccaac tcaattgcct gagcaccgtt ctccccttct
     3301 ccgacaatct cgatatcctt tgcagccgaa agcagatttt ttatgcctgc acgtacccca
     3361 ggatggtcat cgacgatcag tacccgaata tgctttttgg tcattgataa cgtctcgtca
     3421 ac
//
LOCUS       DASTJP010000892        34552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1356601, whole genome shotgun sequence.
ACCESSION   DASTJP010000892 DASTJP010000000
VERSION     DASTJP010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 34552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34552
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356601"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFQ23_25925"
     CDS             complement(<1..568)
                     /locus_tag="VFQ23_25925"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9910109.1"
                     /translation="MRKLIVSNFVTVDGYYEGKDKSINSLFDYFHEDYAGDNKFDYYN
                     AELLRACDFLLLSRNSFLGNKEYWSGVLNNPKFTPIRQETAQLFKSIEKIVISDKLTS
                     EELAPWENTRIISRANAYKEIAELKQQPGRDILVLLSRLLWNDLLVHDLVDELHITYF
                     PLIAGEGSSIFVGRPPVSLKLIQTRSWHD"
     gene            complement(666..1052)
                     /locus_tag="VFQ23_25930"
     CDS             complement(666..1052)
                     /locus_tag="VFQ23_25930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910110.1"
                     /translation="MFRRIELMDQIGATDFQNMIQGMQERGEPIGRDVKRFWKSGIMG
                     FGNETVTAAWIKLNSPRRKIFKNCRFYFTEAGWRRYGRPTVAACQQTGQLYRVLCIKE
                     KSVEVVYRDELQVAVRPKKNKKAPTS"
     gene            complement(1083..1931)
                     /locus_tag="VFQ23_25935"
     CDS             complement(1083..1931)
                     /locus_tag="VFQ23_25935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET9910111.1"
                     /translation="MTPNRSFQLVDVFHDEPFSGNPLGVVLNADGLSTEEMQRITRWL
                     NLSETTFLLQPTTPEADYRVRIFTLEREMPFAGHPTLGSCHAWLALYGNPQNKTEFIQ
                     ECGAGLVPLRRTQGRFAFAAPPLIRGGEVEESKVQELAEFLHIDRSIIIDAQWADNGP
                     GWVVVLLESAEEVLALNPRRDYPNRIDVGVVGPYPKDNPIAFELRAFFSDHNGGIVED
                     PVTGSLNASVAEWLLSSGRATAPFIASQGTRLSRNGRIFIEQDEAGKVWVGGKTTTLF
                     TGICSF"
     gene            complement(2067..2711)
                     /locus_tag="VFQ23_25940"
     CDS             complement(2067..2711)
                     /locus_tag="VFQ23_25940"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET9910112.1"
                     /translation="MNNDLIQFILTAFSMLLVVINPLAVSPVFVAVTANLTPDQRRAT
                     LTRAVLSAFGVALFFLFVGRAFLSYMGVSVQAFAISGGILLFSMAFPMLMGQRSQLQA
                     PSSGETHTAATDVAIFPLTIPQLAGPGTIATILLLATQVGADWVRLGILTGIIAVIYL
                     IAWPTLFMAERVMNRLGEDVIHIITRVLGLVLAALAVQYILNGLTGFYQALIRQ"
     gene            complement(2850..3668)
                     /locus_tag="VFQ23_25945"
     CDS             complement(2850..3668)
                     /locus_tag="VFQ23_25945"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HET9910113.1"
                     /translation="MPIYMDRHYVEGATKHTVALAHEKDLQLQDKYDVNFLTYWFDES
                     RSTAFCLVSAPDKEAVNKTHAEAHGMIPNEVIEVDPSVVEAFLGRIKDPAPAENSDVA
                     VEPGFRVIMFTDLKDSTAMTLRLGEARALHLLHVHNAMTRNALKDFDGREVKHTGDGL
                     MASFISIKDAVRCAIAIQQAFMAYNESNPETPMYVRIGLSAGEPVEDENDLFGTAVNL
                     AARTCAHAEPGRILATQVVHDQYPGERYLFSDIGEITPKGFDDPIRVYDIKCVT"
     gene            complement(3854..4891)
                     /locus_tag="VFQ23_25950"
     CDS             complement(3854..4891)
                     /locus_tag="VFQ23_25950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9910114.1"
                     /translation="MQLKNDEIQVGDAPAIAGLTFRRFRGEEDYPKMIAVIAASAEAD
                     KIERVDTVEDVARVYSHLVNCDPYQDMIFAEINDEVIAYSRGSWFQEENGPRIYASMG
                     FLVPAWRRKGIGSTLLRWVENRLRAIADSHAIVEKSFLQGFADSENVGLATMLEKNGY
                     KPIRYVVQMVRPNLENIPDFQLPDGLDVRPVLPEHYRAIWEADAEAFRDHWGYAEPTE
                     VDYKDWQENKIIFQPDLWQIAWDKNTNEVAGQVRTYINASENEKFNRKRGYTEFISVR
                     RPWRKQGVARALIVRSLRLQKERGMTESALGADSENISGATRVYEDCGFHVTKRSAIY
                     RKPIKPNLSES"
     gene            complement(5019..5423)
                     /locus_tag="VFQ23_25955"
     CDS             complement(5019..5423)
                     /locus_tag="VFQ23_25955"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET9910115.1"
                     /translation="MEFDELYQKAKSVLNPRRLSPEAEAGGVGAALLTESGRVYTGVC
                     IDTSSSMGFCAEHAAAGTMITAGENRVVKMIAVNWDGRVLPPCGRCREFISQLHPDNP
                     NAEVMVVEGKIVPLRELLPYDWRSVAAAKDIQ"
     gene            complement(5440..5934)
                     /locus_tag="VFQ23_25960"
     CDS             complement(5440..5934)
                     /locus_tag="VFQ23_25960"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9910116.1"
                     /translation="MKKAISPDQSPANIAKVIEILTETPQKLEAVSKVLTSEASRQPL
                     GDGERSFTQDLAHLLNGEARSSEMIYQALLSNEPAFADIHPDRNLGKLLRFDLLEFKD
                     LLAYFKLRRAVLLRVLSGLKEADWSRSIREEGNQRKESVYWRARAIAMHELDHLGDLE
                     RKLT"
     gene            6578..8986
                     /locus_tag="VFQ23_25965"
     CDS             6578..8986
                     /locus_tag="VFQ23_25965"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9910117.1"
                     /translation="MNSHYRVVIIGGGIVGTSVLYHLTKLDWKDVLLIERDELTSGST
                     WHAAGGFHAQNDDPTIASLQGYTIRLYKEIEAESGQNVGLHMTGGVSLATTPERWEML
                     KAEKSRFQTMNMETHLITPQEIRAMAPITDLKDVLGGLYDEFEGYMDPHGTTHAYAGA
                     ARKRGGEIVVKNRVLELHQNAEGQWEVVTEQGALTAEHVVNAAGLWARKVGNMVGIDL
                     PVTPMQHHYLVTEDISELVALDREIISVTDLEGFTYLQPDGKGVLLGVYERKPRHWKT
                     EGADWDYGMTLLPTDINRISPELEIGFNRFPVLQHVGIRKWVNGAFTFTPDGNPLVGP
                     VPGLKNYWVACGCMGGFSQGGAIGLTLANWMIHHDPGYDIFAMDVARYGKFASADSYL
                     KAKTAQFYARRFVISYPNEELPAGRPLKISPNHEALKEEGAIYGVTWGMETPQYFANQ
                     TDFVEPPTLRRSNAFDFVAAEVTSTRQAAGMFESAVYARYEVSGVRAESWLDQLVASN
                     LPKVGRVKLAPMLNQNGKLMGDLTISRLADDRFWIIASYYLQEWHMRWFNDHLPSSGV
                     SIKNLSDHWMGFALSGPNSRKIVSQLVEADLSNPAFPFLHCREMDVVSTKAMVARISL
                     TGELGYEINVPVDHQRALYNALMKAGKDLGLVNIGNRALDSLRLEKSYGIWNAEFTQG
                     YTPGMSYLNNFIAYDKGDFIGRNAALKERETGATQTLVTLRVDTIDAEPAPYSPIKQD
                     GKVVGFVTSSAYGHYVQQSLVLAYVDNEALHGDLTVDVIGEPRPCVILKEPAYDPKGQ
                     MMRM"
     gene            complement(9088..11094)
                     /locus_tag="VFQ23_25970"
     CDS             complement(9088..11094)
                     /locus_tag="VFQ23_25970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9910118.1"
                     /translation="MASRYQVQIHYNLRLQMRDGIELSANLWQPVRMHPDENFPAILE
                     MIPYRKDDHRYNTDHRHGQYWAERGFAFCRLDVRGTGSSDGIAYGEYMPEQTQDGYDA
                     VEWLARQEWCNGNVGMWGISWGGFTSIQVAKLQPPHLKAIIPMYATDDRYLDDVHYLG
                     GCVSLADYSQYAVSMVGMNAMPPQIEYTPGWAEKWKARLEATPFWLFEWLKQQTDGPF
                     WRSGSLAPEYDQLQCAIFNIGGWMDPYVDPALRMQAYCTAPSKTLIGNWVHSYPIHAY
                     PAPNLDHLHEMERFFAHWLKGEATGVMDEPPLTLFRREYTQPEAFPARFNGEWVSLSR
                     FPVTESTLTNFYLGDKTLSSSPSNSQPDVLPHMPAHGTRASLCWGAGHTPNGLARDLR
                     PDEALLPTYTSEPLEEPLDILGFPEAVVHVSCDSPVANIIVRLTDVAPDGTSSQVTAG
                     ILNLTHRDSHSDPQPLEKDMIYEVHVKMRSTAYRFLPGHRIRLSIASGYLPVVFPAPY
                     PFILTLHHDERHPSQLNLPVVPASVYLRPPTFKTTPPELIPASGGIDDEPVWEIVEDV
                     LHQTTTVRIYEGGSSILPNGRVDYNSERIEITSHVVDPLRTRMYNEVIYRLDDHGYKI
                     EIIASGTKRATEKEFHLDVQLLVTLNGAEFFRKSQIESIPRRLV"
     gene            11376..12902
                     /locus_tag="VFQ23_25975"
     CDS             11376..12902
                     /locus_tag="VFQ23_25975"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HET9910119.1"
                     /translation="MTSLPPVSKPKRPSPLLSIPFRQPQNPWTPVDILTGEQVEQLHN
                     ASMEILENTGIDFLDDETLTLWEKAGAKVDRAARHVWIDRGLIESAIASAPAEFDWYA
                     RNPAHNLHIGGNTFAFGPCGGMVYASSLEEARHPGTLADYDNFLRLTQMAPVIHFGPW
                     EQVTPQDVEVSERHLQRLRSGILLTDKVLLEAAHGRIISTDNIALAKIAFGANSPPGP
                     IIGDVINVNSPLRYDKRMLGGLITYARAGQVTMITPFILAGATSPITMAAALAQQNAE
                     TLAGVALTQLVNPGAPVLYGGFTVDMDMRSGNPAFGTPTGAWAALAGAQLARYYKLPY
                     RGGGGLTNSHLPDAQAVQESQWMLWSAIMGHAHVLMHSAGWLEAGLTASFEKFILDVE
                     SLAIFQDFFHGFEINTETLALDMIAQVGPGGHNLGTPHTMKRFRHAFYESTINTRQNN
                     KAWMDAGGLDATQRAYVIWKQWLMEYTPPPLDPAIREEVEAYVERRSIELNGVELYSD
                     "
     gene            complement(12957..13154)
                     /locus_tag="VFQ23_25980"
     CDS             complement(12957..13154)
                     /locus_tag="VFQ23_25980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910120.1"
                     /translation="MPDKTISTEQTTENIAPIMQAATQSIRAVRENSVKHSAKASGHR
                     FRDRWLKPGDVKYKPKQRVLA"
     gene            complement(13510..14289)
                     /locus_tag="VFQ23_25985"
     CDS             complement(13510..14289)
                     /locus_tag="VFQ23_25985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9910121.1"
                     /translation="MQDQITNLENVNVIITGAARGLGAAIALGLSDLGCNVILCGRSL
                     DALRVTAGHIEARIKRRPRSYEIDLANAERVEEAAKEINTDFQSIDILINNGAAWLGQ
                     RDAPYEATEVMNTINSAITGTFLFTQALIPALQKSARPDVVNIGSVSGLTNVNLYSTS
                     VPFYAAKHGQTALADGLRQMFLGTPIRSICIHPPWIQDISPLDEEWSTVSSRTKAEMV
                     TNRDILEAVIYAITRPRHVTIASMFIDSDVKGMDYRSHTKS"
     gene            14394..15059
                     /locus_tag="VFQ23_25990"
     CDS             14394..15059
                     /locus_tag="VFQ23_25990"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET9910122.1"
                     /translation="MGTRTHDVIKGRAKYVLILIVLLNMLYPITGSGSTISLIAFAAL
                     YAGLFIVGIFITSDSRAQITWSISVAVIWLVMTVVWALDPTSFWKTQVSSFILIVFHS
                     TIILVLMRYIFTAEQVTADVIYAACAVYLLLSFFFVPVYGMLEAALPGSFVDNTLGSP
                     VHWQQFVYYSLVTLSTAGYGDILPANMWARMLAGLEATIGVLYVAILVARLVSLYEVR
                     RRQ"
     gene            15182..15589
                     /locus_tag="VFQ23_25995"
     CDS             15182..15589
                     /locus_tag="VFQ23_25995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910123.1"
                     /translation="MDNQNTATNLQSKKWRGSLIQLLRLLGGAILGYLISFALLIFDG
                     TAFLPLLGKIIHGQEELFELLFFYALYDSLRSMGAVHGMAEFVYTSLWALIGALLMSG
                     RRKQIRIGVIFLILYVIVGCLAYSILVMLRVPT"
     gene            complement(15677..16636)
                     /locus_tag="VFQ23_26000"
     CDS             complement(15677..16636)
                     /locus_tag="VFQ23_26000"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HET9910124.1"
                     /translation="MTWNIVIACIDTRNPPEALNALAGLDVAVHRVIGFHDERQASLY
                     READAIITEMHPVTDVIMETFSGCRIIACASTGYDYVDHASAGKRGIWVTNCPGYCTD
                     EVATHTIALLLSQVRRLPQVRQLVRANEWDPRPVRPMHHPRNQTLGIIGWGRIGRSVS
                     AKAMALGFQVIANDPYVDPKQMLDAGVKPVEKETLMRESDYVTLHTPLTEETHHMIDE
                     HLLSLMRPSAYLINTARGLLIQEEALLDAVQNDRIAGAALDVLSVEPPSPEHPFLHDD
                     RFFITPHSAWCSEEADAAVWEWAAGDIAYALRGERPPHAVNEI"
     gene            complement(16800..18611)
                     /locus_tag="VFQ23_26005"
     CDS             complement(16800..18611)
                     /locus_tag="VFQ23_26005"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HET9910125.1"
                     /translation="MVKGKYWLHGLILIVMMFGFSPPAAAQGPSDHTEVFYISNVFDA
                     ETRSAIAASGALILEVGHDYVLVEATAQEKRAIERQTGKTIAKPTLEQALPLAFPPAD
                     SAYHDYAEMVAEIQQAELDHGAIFDLFSLGTSYEGRTIWAGKISDNVGTDEAEPEVLF
                     THHQHAREHLTVEMALYTLGILTNEYGSTQQITDLVNGREIWMVFDMNPDGGEYDIAT
                     GSYRSWRKNRQPNAGSSAVGTDLNRNWDYRWGCCGGSSGTTSSETYRGSAPFSAPETQ
                     RVRDFVNSRVIGGQQQITVAIDFHTYSELVLWPYGYTFTDVPADMTQDDHDVLVAMGQ
                     AMAATNGYTPEQASDLYITDGTINDWLYGVHGVLNYTFEMYPRTSSQGGFYPPDEVIP
                     TQTSRNRAAVLYLLEQADCPYDVIGKGSLYCGSGNPPVNTGQLSPSANAAVTSGSGDN
                     NGFEVNPGNALANDGLFAVDNNSGSNNNTSCTSNRKDRHTFYNYNFSIPSGAAITGIE
                     VRTDGRADSTSGAPRFCVEISWNGGASWTAAKTTSTLTTSEATYLLGGLADTWGRTWS
                     SGDFTNANFRVRLTSVASNTARDFSLDWISVQVRYVP"
     gene            complement(18709..19155)
                     /locus_tag="VFQ23_26010"
     CDS             complement(18709..19155)
                     /locus_tag="VFQ23_26010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910126.1"
                     /translation="MFNVTTRNQDDQIDIQHKNSITTVDIHSPTGIGSATLQLESGAM
                     PEKIVLRLHLTGLEQFKLVSEQTTLTASGSSSEGFNLTGQSVLAAGNEYVITPIDPLW
                     TKVEIVSGQGSKEIPLGEGYFEITIPNEFIRRAGNSFEIQWINFFR"
     gene            complement(19349..19777)
                     /locus_tag="VFQ23_26015"
     CDS             complement(19349..19777)
                     /locus_tag="VFQ23_26015"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HET9910127.1"
                     /translation="MTPKKQLWVCPTCGRQFVSVKRNHSCGLYSLEDHFAGKEALVHE
                     LYDLLFEKLQLFGPVKAFPVKTRIVFQAEVQFAAAVPRKRWLEGYLWLQRQAVHPRIH
                     KLEMGVFRDYGHIFRLVRFDDLDEELISLLHEAFILGCNI"
     gene            complement(19778..20425)
                     /locus_tag="VFQ23_26020"
     CDS             complement(19778..20425)
                     /locus_tag="VFQ23_26020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9910128.1"
                     /translation="MKPNRIILIRHGQSQGNIDANQYQTVPDYALLLTQKGIEQSTKA
                     GVQIKDIIGAESLHVYLSPYVRTRQTFQHLRISLEANIDKVVEDPRIREQDWGHLRHP
                     DENEEIRRQRDGFSTFYYRIPDGESGADVYDRVSTFMETLHRDFKKPSYAQNTLIVTH
                     GLTLRLFLMRWFHWTVEEFENLRNPRNCQVVVMQKNEKDRYELINELEKRKPSVG"
     gene            complement(20438..21481)
                     /locus_tag="VFQ23_26025"
     CDS             complement(20438..21481)
                     /locus_tag="VFQ23_26025"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET9910129.1"
                     /translation="MEDLYYSEYIELDKILNSQHPRSFESMEDGNDEMLFIIIHQAYE
                     LWFKQIIFELDLARNIFIRGDINDNSSDMSNVVYKMKRIGKILELINQQVNILETMTA
                     LDFLEFRNVLLPASGFQSKQFRLIEAKLGLKMEQRHKSKYYKASRQGSLSEADLQEVN
                     QAESQSTLKELIIQWLERMPYLEERYWSDYQQASQSDFKDRHKFWLDYRNAYQKSLSK
                     NESGRLQEFDDVFFEKGRGEISPAAMRAALFITIYRDLPIFQFPFELLNTLSEIDELL
                     SNWRYRHFMMVRRMIGIRIGTGGTSGAGYLEGTLSQHHTFREITEVATFLIERSKRPI
                     LPKALKDKAGFNL"
     gene            21665..22372
                     /locus_tag="VFQ23_26030"
     CDS             21665..22372
                     /locus_tag="VFQ23_26030"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET9910130.1"
                     /translation="MTSEAVSRTNGAALWVKALIFDFDGLILDTETPDFQVWQTIYQE
                     HGFELPHEEWGKIVGGFGLAHFDAAEHLTSLADRELDAVSLRDRHRSESHALTLEQPI
                     LPGVLNYIQDAKRLGLKLAIASSSEHSWVDTHAKRLGIFDHFDAVICADDVSPGRTKP
                     NPDLYLLALNRLNVQNHEAIVLEDSPNGVQAANRAGIFVMAIPNPVTSLLKIEGANLV
                     FKSLADLSLPELLDKVK"
     gene            22396..23403
                     /locus_tag="VFQ23_26035"
     CDS             22396..23403
                     /locus_tag="VFQ23_26035"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9910131.1"
                     /translation="MTTEKFRWQGIVSKYAYPETWRSSWQVANTLIPFIVGWYLMYRS
                     LEIGYWLTLILAVPTAGFMMRLFIIFHDCCHGSFFKSMKANDRLGLVLGVLVFTPFYE
                     WKHSHAIHHATAGDLDRRGIGDVYTMTVEEYLAAPWYKKFGYRIMRNPLILFTVGSFI
                     VFTVTHRFWERGSGKRERSSVIWTNIALAAFIGWIVIEIGWVAFLLVEIPVLLLACGA
                     GVWLFYVQHNFEPTYWERHTDWEFFNAGMDGSSFYKLPKVLQWFTGNIGFHHIHHLSP
                     RIPNYKLEECHNENPVFQIEPLTFGASLKSLFFRLWDEKEKMLVGWDALNRYRQKADA
                     S"
     gene            complement(23472..24527)
                     /locus_tag="VFQ23_26040"
     CDS             complement(23472..24527)
                     /locus_tag="VFQ23_26040"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9910132.1"
                     /translation="MSTPDSTANPAVPDNQNYAALKRLILEKGLLEKQPTFLTYKILL
                     SAGLLAVSIAILFLTDNLWIQILNAVYMAFVFGQMGFIAHDTGHRQGFRTTKQNDFFG
                     ILHANLLIGMSYGWWLEKHNQHHAHPNQEDMDPDIAIPVIAFTEKAALEKRGIPRFIV
                     KYQSFFFFPLLLFEAYSLHVGSIRFFIQRTTWKYRLVEILLLTLHFVWFFSLIFLALG
                     AWKGIVFILVQQALFGLYMASVFAPNHKGMLVVGKNETMDFLRLQVLTARNVKAHPIT
                     DFWYGGLNYQIEHHLFPSLARNQLREAQTIIRKFCDDLEISYHETSMFHSYVEILEYL
                     HVVSAPLRQKELAAEAI"
     gene            24659..26233
                     /locus_tag="VFQ23_26045"
     CDS             24659..26233
                     /locus_tag="VFQ23_26045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910133.1"
                     /translation="MDDRELLEKYEPVLRFAKSERFFPMAVEPYIEKCTIFPSGPQGV
                     IGLLSHLNEPLVKRLGKLESGEYFLRFVNDPLIDSDIWVWWGVLSSAAAVGGGFLRGW
                     DGVVGAAMFALLAAFVVFIQASPIRLRFFLAASAAIIFFVLGALPVWFFLTPHVFISV
                     AIEYLILLPIYLLVLFFLTIRTMKFIFDYIIPEAPGVIMDMLSRSTETVARKSFWQYA
                     EILKNHPEPVYYGRVAAWQDVEGNQWKSLQYHFFYAFNDWRLAANGINHHEGDWEMVA
                     VYLKNNEPHSLLCSQHGTGAMELWENVRRVKNMDGTETMHPLIYVALGSHANYTRPQV
                     IRSASLFHEGLFQRFIYWTDGLIHFLFMLFNPSQKARQIALNQLATHPAMALTEESFE
                     NLRDEKDHYLVSLPMEIATGDGLRIGYAGDTRGEKIGISSSYLKRVMSNRKVVHPGLK
                     EWKQVLLSPEPDWVQYKGLWGVKSLMKDESGPPGPKWDRPDKLFAVYPRKRWDQALEW
                     LTELENILMSKNKKKT"
     gene            complement(26230..27177)
                     /locus_tag="VFQ23_26050"
     CDS             complement(26230..27177)
                     /locus_tag="VFQ23_26050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9910134.1"
                     /translation="MTSSSFTLLEASHWTDYALLDSGDGLKLERFGNYVFVRPESQAM
                     WKRSLNNEWKNAHAVFQPTGEESGGHWDFKKKVEERWEMRYVLDPSVTPTPSPIGRGE
                     LKFWAMTTPGRHLGVFPEVAAHWDWFSSLIQHEGHKGHDVNILNLFGYTGLATLAAAS
                     AGAKVTHVDASKKSVSWARENQALSDLTDKPIRWIVDDALKFVQREARRGVKYDGIIL
                     DPPKFGRGPKGEVWEVYKSLPSLFEACRACLSDKPLFVVTTLYAVRASAIHVAQAMED
                     MMSRFGGKIEMGELVTREQSAGRLLSQAVYARWENKVEG"
     gene            27333..27779
                     /gene="mraZ"
                     /locus_tag="VFQ23_26055"
     CDS             27333..27779
                     /gene="mraZ"
                     /locus_tag="VFQ23_26055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET9910135.1"
                     /translation="MFLGQFQHNLDDKGRLMIPARFRELLAAGAFITQGFDRCLMVMT
                     DTYFRQVYERISAMNLADPMARLLRRLILSNAYPVEVDKVGRILLPQNLRQSILLESE
                     AILAGQGEYFEVWNPAEWNAQMAQLQDIETNNQRFAALDLANYSGS"
     gene            27826..28764
                     /gene="rsmH"
                     /locus_tag="VFQ23_26060"
     CDS             27826..28764
                     /gene="rsmH"
                     /locus_tag="VFQ23_26060"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET9910136.1"
                     /translation="MNESPHQPVLYKEIIHALQPYAGGRYVDGTLGAGGHARGILEAS
                     VPDGQLLGLDVDPQALALARKILAPYEERVHLAQASYVSLSDQLAQLGWAAVDGILLD
                     LGASSMQFDTPERGFSFAHDAPLDMRFGPSMLKTAADLVNKYDERELADLIYKYGEER
                     ESRKIARAIVKARPLHTTRELVAVIETVSPRRRSFDRLRSIHPATRTFQALRIAVNEE
                     LLSIENVLPQAVASLGSGGRLAVISFHSLEDRIVKDYFREQSKDLVNPPYEQIYKEER
                     KATLKEVNRKPITPSDEEIKSNPRARSAKLRIVEKL"
     gene            28850..29305
                     /locus_tag="VFQ23_26065"
     CDS             28850..29305
                     /locus_tag="VFQ23_26065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910137.1"
                     /translation="MQAAQIIHAHKQAPWRVQRQWVGAFLLAVIGMAMIAALYLDVTA
                     RAAVTGREIQELRIEITTIQRTNADLETRLANMTSTVEMQRRALALGYRPVKPGELDY
                     VAVPGFTVPEPEILLVADDAPPPTQSLPAEFSQSLIEWFDERIRFGGAP"
     gene            29302..31038
                     /locus_tag="VFQ23_26070"
     CDS             29302..31038
                     /locus_tag="VFQ23_26070"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9910138.1"
                     /translation="MRQQYAIRSLILVACLAFVGVAVIVQMVRIQNSEQAQIFLSQGD
                     RYAGEFHMFYPERGEIYDRHGHLLAGNRTVYEVGVSLSEMESPESMASILSSYLGLAF
                     EDVYDKLTNSPETWEYIVVQDYVGAETVTAIQALMQQFDETDDQRLSGLGFKPHFQRS
                     YPETALASNVLGFVTRDGRGYFGIEEKYNDLLAGNPVQVWVPSDPNKALEIPRVPNGT
                     TLVLTLNRDLQSSVETILDDALTEYGAENGTIIVMDPRNGEILAMAATPRMNLNDYSN
                     YFSLYDDGSQYNRSIGMAYEPGSVIKVLTMAAALDAGTVQRETPFIDTGAIQVGGITI
                     QNWNRDAWGQQNMVGCLQHSLNVCMAWISSQMGSQSFYGYMERFGLGHPTGVDLAGEA
                     VGRLKVPGDTDWYPVDLGTNAFGQGISVTPLQMLMAVSAIANDGHMVTPHVLYSMVRD
                     GHQYNVPSQYAGSPISSETANTLNEMLAVSLEQESSQALLAGYRLAGKTGTAQIPTDF
                     GYDAFHTNVSFIGWGPVDEPQFMIYVWLQKPSSSIWSSETTAPVFSQVAEQTVIQLNI
                     PPDLVRNQLSIK"
     gene            31087..32511
                     /gene="murF"
                     /locus_tag="VFQ23_26075"
     CDS             31087..32511
                     /gene="murF"
                     /locus_tag="VFQ23_26075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET9910139.1"
                     /translation="MLTLADAIEALTSFRPLAVTVITEAVIDSRQVIPGSLFVAIPGE
                     QVNGHDFIGDAFKRGASFALIQKEVDASFRTVDLRSVLSAESLSERDFTTPLCLRVEN
                     TVTALQQIARYWRRKLDLRVVGITGSVGKSTTKEMATEVLSTRYRTLKSPGNLNNEIG
                     LPLTILKLSSGHQRAVLEMGFYVPGEIAFLCDIALPQVGVVTNIGTVHAERAGSQEAI
                     ARGKAELVQALPPGPEGIAILNFDDPWVRKMEEKTKARVFFYGLSPEAHLWADQVVGL
                     GLDGIRFRLHYAGETLHVKIPLIGRHSVHTALRAAAVGLAEGMNWQEILEGLMQGHTQ
                     LRLAAVRSETGALLLDDTYNASPESMLAALNLLDELDGRKVAVLGDMLELGPYERGGH
                     EMVGLRAAQVANILLTLGERAHIFGEAARRAGMKKSSIIEFDDFDPLVEWLKSNLKKD
                     DAVLIKGSHGLRMDRITSLLEVRS"
     gene            32514..33506
                     /gene="mraY"
                     /locus_tag="VFQ23_26080"
     CDS             32514..33506
                     /gene="mraY"
                     /locus_tag="VFQ23_26080"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET9910140.1"
                     /translation="MNHTALVLGLAMLSFVMTVIWGTPLLRVLRHYRVGKVIRVEEPD
                     RHRVKMGTPTMGGVLFILPAILLTGLLNAAALIGLDVTGQSVLVPLIVLVSYAVLGAL
                     DDWEGIRGKRRGEGMRARTKFAAQVILAVGTAIVLKYVLEVEEMYLPGVEGEIELGWW
                     YIPIAAFVIVSESNAVNFTDGLDGLSGLITATAFAAFGGIALIIGYTYLAQFCFIIVG
                     ALFGFLWFNVHPAELIMGDTGSLALGSTLAVVALMTGRWPLLIIIAIIPLSEALSVVI
                     QVLYFKLTKGKRFFKMAPIHLHFELLGWSETQVVQRFWLIGLLAAMVGMGLAFV"
     gene            33507..>34552
                     /gene="murD"
                     /locus_tag="VFQ23_26085"
     CDS             33507..>34552
                     /gene="murD"
                     /locus_tag="VFQ23_26085"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9910141.1"
                     /translation="MKTNWNGKRVLILGAARQGLALARWLSLHGAHVTLNDAHSEDEL
                     RVARESLAEYKIDWAFGGHPLELLDSTDVLCLSGGVPLTLPIVAEAVKRGIPLSNDSQ
                     IFMEVAPCKTVGITGSAGKTTTTTLVGRMSRNVYGDRAYIGGNIGDPLINYVDNMNAD
                     DIAILELSSFQLDQMTISPNVAAILNITPNHLDRHGTMEAYTDAKARILEFQSEQDVA
                     ILSRDDRGSWNLNDKVRGNLYAFSSQALDKALNGAYLQDGWLNLRDGNLLLPLISRGH
                     ISLRGDHNVSNVLAAFAIGHAAGFAPDPMMQAVKDFHGVPHRLEFVRELRGVCWYNDS
                     IATAPERSMAAIRA"
BASE COUNT         8096 a         8550 c         8966 g         8940 t
ORIGIN      
        1 agtcgtgcca ggaacgagtc tggattagtt tcaatgagac gggcgggcgt ccgacgaaga
       61 tggaggagcc ttctccggca atgagcggaa agtaggtgat gtgcagttcg tccaccaggt
      121 catggaccag caggtcattc caaagcaaac gactcaggag cacgagaata tctctgcccg
      181 gctgttgttt gagttctgca atttctttat aggcatttgc tcgtgagatg atgcgcgtgt
      241 tttcccaggg agccaactct tcagaggtca gcttgtccga aattacgatt ttctcaatgg
      301 atttaaaaag ctgtgcagtc tcctgccgga ttggcgtgaa cttgggatta ttcagcacac
      361 cggaccaata ctctttgttg cctaaaaacg aattccgact caacaaaagg aaatcgcagg
      421 cacgcaagag ttctgcgttg tagtagtcaa atttattgtc acccgcatag tcttcgtgaa
      481 agtaatcgaa caatgagttg atgctcttat ccttcccctc gtaatagcca tccacggtga
      541 caaagttact gacaataagt tttcgcatag atcacttcgt tatgttttcc tggagatgat
      601 ttttcataca gccggcggct gttccagaac tatagcacgc ttgttctacg aagtcaacga
      661 ggcgattacg aggtgggtgc tttcttattt ttcttcgggc ggactgccac ctgaagctcg
      721 tcgcgataga caacttcaac cgatttctct ttgatgcaaa gaacccgata gagctgacct
      781 gtctgctgac acgcggctac tgttggtcgg ccatagcgcc gccaaccggc ttcggtgaaa
      841 tagaaccggc agtttttgaa gatctttcgc cgtggactgt ttagcttaat ccaggcagct
      901 gtgacagtct cattgccaaa gcccattatt ccggatttcc aaaagcgttt cacgtccctg
      961 ccaatgggct cgccgcgttc ctgcatgccc tggatcatat tttgaaagtc cgttgcacct
     1021 atttggtcca ttagctcgat tcgtctgaac atagtatgct attcctccct ggaaatttgt
     1081 ttttagaatg aacaaatgcc agtaaaaagt gtggtcgtct taccgcccac ccatactttt
     1141 cccgcctcat cctgttcgat gaagatacga ccatttcgac tgagacgtgt tccctgggaa
     1201 gcgataaagg gagccgtcgc gcgtccactg gataaaagcc attcggccac cgacgcattc
     1261 aggctgcccg tcacgggatc ttcaacgatg ccaccgttat gatcactgaa gaaggctcgc
     1321 agttcaaaag cgatggggtt atccttcgga tatggaccca caacgcctac atcaatacgg
     1381 tttgggtaat ctctgcgcgg gttgagcgcc agcacctctt ctgctgattc aaggagcacg
     1441 acgacccaac cgggtccatt atctgcccat tgtgcgtcga taataattga acgatcaatg
     1501 tgaagaaatt ccgccaactc ctgaactttg gattcctcga cttctccacc gcggatcaga
     1561 ggcggtgcag cgaatgcaaa tctcccctgg gtgcggcgga gaggcacgag acccgcgcca
     1621 cattcctgaa tgaactccgt cttattctgt gggttgccat agagggcaag ccacgcgtga
     1681 cagctcccca gtgttgggtg accggcaaat ggcatctccc gctccagcgt aaaaatgcgg
     1741 acgcgatagt ctgcttctgg agtcgttggt tgcaggagga atgtagtctc tgaaagattc
     1801 agccagcgtg tgatccgctg catttcctcc gtcgaaagtc catcggcatt caaaacgact
     1861 cccagcgggt ttccggaaaa cggttcatcg tgaaatacat ccaccagttg aaaactgcga
     1921 ttcggggtca ctgcctacac ctccgtattg gtttttcgct cagctatgaa atcttcgcat
     1981 caatactcga ttgaggcgaa ttatacgccc tatagagaat caaaacggga ctgggttcag
     2041 ccaccgtccc gttttcttaa atgaatttac tgccttatta gagcttgata aaaaccagtc
     2101 agcccgttca aaatatattg caccgccagc gccgccagaa ccaggcccag cacccgagta
     2161 ataatgtgga tgacatcttc accgaggcga ttcatcaccc gctccgccat gaacagcgtc
     2221 ggccaggcaa tcaggtagat gactgcaata atgcccgtaa ggattcccag gcgtacccaa
     2281 tctgcgccga cctgggttgc caacaacagg atggtggcaa tggtacctgg tcccgccaac
     2341 tgcggaatcg taagcggaaa gatcgctaca tccgtcgctg ctgtatgggt ctccccgctg
     2401 gaaggcgcct gaagttgtga tcgctgtccc atcagcatcg ggaaggccat actaaaaagc
     2461 agaatgccac cactaatcgc gaaggcttgc actgacacac ccatgtaact caggaaagca
     2521 cgccctacaa ataaaaagaa taaagccaca ccgaaagcgc tcaggacggc ccgagtaagt
     2581 gtcgcgcgtc gttggtcagg agttaagttg gcggtcaccg ccacgaacac gggggatact
     2641 gcaagcggat tgatcacgac cagtaacata ctgaaagcgg tcaggataaa ttggatcaaa
     2701 tcattattca tggcattctt acatgccttt cgatttatac atttagttta ttggcttgcg
     2761 ataaatcgta cttcgctttg ttacgcggaa accgcagtct tcatacacac gtgtcgcgcc
     2821 gctgatattc tcactatcgg cgccgagtgc taggtcacgc atttaatgtc atacacccgt
     2881 ataggatcgt cgaagccttt cggtgtaatc tcacctatat cagagaacaa atatctttcg
     2941 cccggatatt gatcgtgaac cacttgggtg gcgagaattc tgccgggttc tgcatgcgcg
     3001 caggtacgcg ccgcgagatt gacggctgtt ccaaacaaat cgttttcgtc ttctacgggc
     3061 tcaccagcgc tcaacccaat ccgcacatac atcggtgttt ccgggtttga ctcgttataa
     3121 gccataaatg cctgctggat ggcgatggcg caacgtaccg catccttaat ggaaatgaag
     3181 gaggccatta gcccgtcacc agtgtgcttc acctcgcggc catcgaagtc ttttaatgca
     3241 ttgcgggtca tagcattatg aacatgaagt aggtgcagtg ctcgagcttc acccagtcgc
     3301 aaggtcattg ccgtggagtc cttcaaatct gtgaacatga tgacccggaa gccaggctct
     3361 acggcaacgt cactgttttc cgcaggtgca ggatctttaa ttcggcccag aaatgcttcg
     3421 acaaccgagg gatcgacttc gatgacctca ttcggaatca ttccatgggc ctcggcatgc
     3481 gtcttgttaa cagcttcctt atcgggagca gaaacgagac aaaatgcagt ggagcgggat
     3541 tcgtcgaacc aataggttaa aaagttgaca tcgtacttgt cttgaagctg gagatccttt
     3601 tcgtgagcca gggcaacggt atgctttgtt gcgccttcca catagtgacg atccatataa
     3661 atgggcattt tccggttcct tttgacgcta aaaagagcga tattatctac aaaacaggct
     3721 gactatgatc tatgaagact cagtttgtat gacagcttat aggcagaatt ataagcctgc
     3781 tcataaactt ccaatcaaat caaaaacggg actgtactac atccacagtc ccgtcttgat
     3841 tatggttact gatctagctt tcgcttaggt tgggttttat tggcttgcga tagatcgcac
     3901 tgcgcttcgt tacgtggaag ccgcagtctt catagacacg cgtcgcgccg ctgatattct
     3961 cactatcggc ccctagcgcg gattcggtca tgccgcgttc tttttgcagg cgtaaacttc
     4021 gcacgatgag cgcacgtgct actccctgtt ttctccatgg gcggcgtacg ctaatgaatt
     4081 ctgtatatcc tcgtttgcga ttgaactttt cgttttccga cgcgttgata tacgtacgca
     4141 cctgaccggc gacttcgttc gtatttttgt cccacgcgat ttgccaaaga tcaggttgaa
     4201 agatgatctt attttcctgc caatccttat agtccacttc ggttggttcg gcatatcccc
     4261 agtggtcgcg gaatgcttct gcgtctgctt cccagatggc tcgataatgt tctggcaaca
     4321 ccgggcggac gtccaagcca tcaggcagct ggaaatccgg aatattttcc aaattcggcc
     4381 gcaccatttg aaccacatac cggatcggtt tgtacccatt cttttccagc atggtggcaa
     4441 gccccacatt ttcactgtcc gcaaaacctt gaagaaagct tttctctacg attgcatgtg
     4501 aatcggcgat tgcgcgcagc cggttctcga cccagcgcag cagcgtggaa ccaattcctt
     4561 tgcgccgcca ggctggaact aaaaaaccca tcgaagcata aatgcgcggt ccattctctt
     4621 cctggaacca ggatccacgt gagtaagcga tcacttcatc atttatttct gcaaaaatca
     4681 tatcctgata aggatcacaa ttcacaaggt gtgaatatac acgagcgacg tcctctaccg
     4741 tatccactcg ctcgatctta tcggcttctg cactggctgc aatcacagcg atcattttgg
     4801 ggtaatcttc ctcgccgcga aaacgccgga acgtgagacc tgcgatagct ggcgcatccc
     4861 ccacctgaat ttcatcattt tttagctgca tatgtttctc catttatgat gtttaccaga
     4921 cccacacatt tccctcagaa tccgccatga caagccttcc ctctggacta aaagaacggg
     4981 aacagtatac gccacatctt gagcggagct ggcacttttt attgtatatc cttcgctgct
     5041 gcaacagagc gccagtcata tggcaatagt tctcgtagtg gcacgatctt tccctcaacc
     5101 accatcactt cggcgttggg gttatccggg tgaagctggc tgatgaattc ccggcaacga
     5161 ccgcagggtg gtaagaccct cccatcccaa ttcaccgcga tcattttgac cacacgattt
     5221 tcgcccgctg tgatcatggt gccggcagcg gcatgttcgg cacaaaaacc cattgaactg
     5281 gaggtgtcga tacatacccc ggtgtacact ctgccgcttt cagtgagcag tgccgctcca
     5341 acgccacctg cttcggcttc tggtgaaagg cggcgggggt tgagcaccga tttagctttt
     5401 tgatataact catcaaattc catgaaagcc tccttcgctt caggtcaact ttctctctaa
     5461 atctcccagg tgatccaact cgtgcatggc aatcgcgcga gcgcgccagt acacagactc
     5521 cttgcgttga ttgccttcct cacgaatact gcgcgaccaa tctgcttcct tcaacccgga
     5581 aagcacacgt agcagtacgg cgcggcggag tttgaaatac gccagcagat ccttgaactc
     5641 caggaggtca aaccgcagga gctttcccag gttccggtcg gggtggatgt ctgcaaaggc
     5701 tggttcgttc gataacaaag cctgatagat catctctgag gaacgggctt caccgttgag
     5761 tagatgtgcg aggtcttgtg tgaaggaccg ttcgccgtca cccaggggct ggcgcgaggc
     5821 ttctgacgtc agtaccttgc tcactgcctc cagtttttgc ggagtctcgg tgagaatctc
     5881 gatgactttg gcaatgttgg ctggagattg gtcaggggag attgcctttt tcatttgatg
     5941 gactcagagt ttttgatttt tctcatgcgc tgtgagatat acctcaggat cacgttaatg
     6001 agtgcatcgg atcgtcagtc caagaataaa atcgaacgat aaaattgtag catatccttc
     6061 caggtgcaac tctttccctg cccacttcat tttcaacaac ttgttcctgg cctcccatta
     6121 caaactccct atcctataac gagtcccttc cctctcgccg catgtgggct ggatgccagt
     6181 gatcatggtg agtaacgagg tctggcttct gtttgtccaa gagggcacgc tcctggggtt
     6241 tttctgtgtg acttttaaga gtggcggatt tagtgtgtaa gccacactcc tgcctgattt
     6301 gcttaacaaa tcatgcgggg tcaaaggact cagtggtctc ggtggctaag tggattagca
     6361 ctcactctct taaccactcc ctgactttgt ggctaactat ttttcaggtt gaccagcgtt
     6421 ataatcggca aaatttcata agagcccggt ggtcgagcgt agcagtcagg gccacctata
     6481 cccaggacgt attcgcaata caaaacatcc aattccttgt tggtcttgat actttcgtgt
     6541 ttgaccaatg acctatggat ctgaggagac aatcgttatg aattcgcatt accgggtggt
     6601 tatcatcggt gggggaattg tgggtacgag cgttctctac catctcacaa aacttgattg
     6661 gaaagatgtt ttgttgatcg aacgcgatga actgacctca ggctccacct ggcatgccgc
     6721 cggcggattc catgcacaga acgatgatcc gacaattgct tctctccagg gctacaccat
     6781 cagactctat aaggaaatcg aggccgaaag cggacaaaac gtcggactgc acatgacggg
     6841 cggggtgagt ctcgccacca cgcctgaacg ctgggaaatg ctcaaagcag agaagtcccg
     6901 ttttcagacc atgaacatgg agacccacct gatcacaccc caggagatca gggccatggc
     6961 tccaatcacg gatctcaaag atgtcctcgg cgggttatat gatgagttcg aaggctacat
     7021 ggatccacat gggaccacac atgcctatgc aggtgcagct cgaaaacgcg gcggtgaaat
     7081 tgttgtgaag aatcgcgtgc tggagttgca tcaaaacgca gagggtcaat gggaagttgt
     7141 aaccgagcaa ggcgccctca cagctgagca tgtcgtcaac gctgctgggt tgtgggcgcg
     7201 caaagtagga aacatggtcg ggatcgatct gcccgtcaca ccgatgcagc atcattattt
     7261 ggtcaccgaa gatatttcag aactggtggc gctggaccgt gaaattattt ccgtcactga
     7321 tctggaaggc ttcacgtatc tacaacctga cggcaaaggt gttctgctgg gtgtctatga
     7381 acgtaagcca cgtcactgga aaacggaagg tgctgattgg gattacggca tgactttact
     7441 cccaacagat atcaatcgga ttagccctga actcgaaatt ggcttcaacc gtttccctgt
     7501 tttgcaacac gttggcatcc gtaaatgggt caatggtgcg ttcacgttta cgcctgatgg
     7561 taatccgctg gtaggtcctg tacccggact caaaaattat tgggtcgcgt gtgggtgcat
     7621 gggcggtttc tcacaaggtg gagccatcgg gcttacgctc gccaactgga tgattcacca
     7681 cgatccgggc tatgacattt tcgcgatgga tgtagcccgc tatggcaaat tcgcttccgc
     7741 tgattcatat cttaaggcaa agacagcgca gttttatgcg cggcgttttg tcatttcgta
     7801 tccgaatgag gaattacctg cagggcgtcc gttgaaaatc tcgccgaatc atgaggcatt
     7861 aaaagaagag ggtgcgattt atggggtgac atgggggatg gaaactccgc aatattttgc
     7921 gaatcaaacg gacttcgtcg agcctcccac actcaggcgt tcgaatgcat ttgattttgt
     7981 tgctgcggaa gtaacgtcaa cgcggcaggc agccggcatg ttcgagagcg cggtctatgc
     8041 gcggtatgaa gtctctgggg tgcgtgcaga gtcctggctc gatcagctgg tcgcctccaa
     8101 tttgccgaag gttggacgtg tcaaacttgc tccgatgttg aatcaaaatg gcaaactgat
     8161 gggcgatctc accataagtc gcctcgccga tgaccgcttt tggattatcg cttcgtatta
     8221 tttgcaggag tggcacatgc gctggttcaa tgaccatttg ccgtcgagcg gcgtgtcgat
     8281 aaaaaatctt tcagatcact ggatgggttt tgcgttgtcg ggtccgaatt cacgcaagat
     8341 tgtttctcaa cttgtcgaag cggatctgtc gaatcctgcg ttcccatttt tacattgccg
     8401 cgaaatggac gtggtttcaa caaaggctat ggtcgcccgc atctcgctga caggtgagct
     8461 tgggtacgag atcaatgtac ctgtcgatca tcaacgtgct ttatacaacg ctctgatgaa
     8521 ggcaggcaag gacctgggcc tggtcaacat cggcaaccgc gcgcttgaca gcttaaggct
     8581 cgaaaaaagc tatggcattt ggaatgccga gttcacgcag ggatacacgc ccgggatgtc
     8641 gtatctcaac aatttcattg cgtacgataa aggcgatttc attggtcgta acgcagcgtt
     8701 gaaagaaaga gagactgggg caactcaaac acttgtcacg ttgcgagtcg acaccatcga
     8761 tgctgagcct gcgccgtact caccgataaa gcaggatggc aaagtcgttg gctttgtcac
     8821 ttcctctgcc tatggacatt acgtccagca aagtctcgtg cttgcgtatg ttgataatga
     8881 agcactccat ggtgatttga ccgtggatgt gattggcgaa ccgcgtccgt gtgtgatctt
     8941 gaaagaacct gcgtatgatc caaaagggca gatgatgagg atgtaatatc tcgtcattgc
     9001 gaggagggct ttgcccgacg aagcaatccc aacatattgg tggagattgc ttcgatcctt
     9061 tcaggcgctc gcaatgacgg attgtcttta caccaacctc cgcggaatac tttcaatttg
     9121 tgatttgcgg aagaactcgg ctccgttcaa tgtgaccagc aactgcacat ccagatgaaa
     9181 ttccttttcg gtggcgcgtt tggttccgct agcgattatt tcgatcttgt agccgtgatc
     9241 gtcgagacga taaatgactt cgttatacat gcgcgtgcgg agcggatcca ccacgtggct
     9301 ggtgatctcg atccgttcgg agttgtagtc cacgcgacca ttcggcaaaa tactcgagcc
     9361 accttcatag atgcgaactg tggtggtttg atgcagaacg tcctccacaa tttcccagac
     9421 gggttcatca tcgatgccgc cgctggcagg aattaactca gggggtgtag ttttgaatgt
     9481 tggtggccgg aggtataccg atgcggggac cacaggcaag ttgagctgtg atggatgcct
     9541 ttcgtcgtga tgaagagtca aaataaaggg gtagggtgca gggaaaacaa ctggcaggta
     9601 tccgcttgca atgctgagtc ggattcggtg cccaggcagg aagcgatagg cagtcgatcg
     9661 cattttgacg tgcacttcgt aaatcatatc tttctcaagc ggctgtggat ccgaatgtga
     9721 gtcgcgatga gtgagattta aaatcccggc ggtgacctgc gacgaagtcc catctggcgc
     9781 gacgtccgtg aggcggacga tgatattggc aactggtgaa tcgcagctca catggaccac
     9841 agcttcggga aagccaagaa tatccagcgg ttcctccagc ggctcactgg tgtaggttgg
     9901 gagaagggct tcgtccggac gcaggtcgcg ggccaatccg tttggtgtat gccctgcacc
     9961 ccagcataat gacgcgcgcg ttccgtgcgc gggcatgtga gggagtacat caggttgcga
    10021 gttggaaggt gatgaggaga gcgttttatc gccgagataa aaatttgtca gcgtggattc
    10081 ggtcacagga aaccgagaca ggcttaccca ttcgccgttg aagcgggcgg ggaacgcttc
    10141 gggctgagtg tattcacggc gaaagagagt cagcgggggc tcatccatca cacccgtagc
    10201 ttcgccttta agccagtgag caaaaaaacg ttccatttca tgaagatgat ccagattcgg
    10261 cgctgggtac gcatggatcg ggtacgaatg gacccagttg ccgatcaacg ttttgcttgg
    10321 cgcagtgcaa taggcttgca tgcgcaatgc cgggtcaaca tagggatcca tccaaccgcc
    10381 gatgttgaag atcgcgcatt gtagctgatc atactccgga gccagcgaac ctgatcgcca
    10441 gaacggaccg tctgtttgtt gcttgagcca ttcgaacaac cagaacggag tcgcttcaag
    10501 acgggctttc catttttccg cccagccagg cgtgtattcg atctgtggcg gcatggcgtt
    10561 catgccgacc atactcaccg cgtattgcga ataatctgcg agtgacacac atccgcccag
    10621 gtagtgcacg tcatcgagat aacgatcatc ggtggcatac atcgggataa tcgctttgag
    10681 gtgcggcggc tggagtttgg ccacctgaat ggatgtgaag ccgccccacg aaatgcccca
    10741 catgcccaca ttaccgttgc accattcctg tctggccagc cattcaaccg catcatatcc
    10801 atcctgcgtt tgttcgggca tatattcgcc gtatgcaatg ccgtcactgc tgcccgtgcc
    10861 gcgcacatcg agacggcaaa aggcgaagcc gcgctccgcc cagtattgcc cgtgacgatg
    10921 atcggtgttg tagcgatgat catccttacg atacggaatc atttccagaa tggcgggaaa
    10981 attttcgtcc gggtgcatcc tcacaggctg ccacaggttg gcagagagtt cgatcccatc
    11041 gcgcatttgc aaacgaagat tgtagtgaat ttgcacttga taacgactcg ccataaaata
    11101 tcatcccttt tgcctatgaa gtattttgca atgcgaagcc attataatcg tcaggaatta
    11161 ttaagccaca gagacgctga gtctcagagt ttttaaaatt aggtcttcgc ctgtcattgt
    11221 ggatcgcgac acttatacgg cattgcgctt ggtgcagtgc aggtgtcgct ccctccggtc
    11281 gctcagaatg ctatgcaaaa ggattttcaa agactttctt gtctctgtgt ctcagtggct
    11341 tgctcttaag atcataagta tatagaggat catccatgac cagcctgcct cctgtctcca
    11401 aaccaaaacg cccatcgcca ttgttgtcca tcccgtttcg ccagccgcag aatccctgga
    11461 cgccagtgga tatcctcacc ggtgagcaag tggaacaact ccataacgct tcgatggaga
    11521 ttctggaaaa cacaggcatc gattttctgg atgatgaaac actgaccctc tgggaaaaag
    11581 ctggcgccaa agtcgaccgg gccgcgcgtc acgtctggat cgaccgcggc ttgatcgaat
    11641 ctgcgattgc gtccgcaccc gctgaattcg attggtatgc ccgcaacccg gcccacaatt
    11701 tgcacattgg tggcaacaca tttgccttcg gtccgtgtgg aggcatggtc tatgcgtcga
    11761 gtttggagga ggcgcggcat cctggcacgt tagccgatta cgataatttt ttgcgtctca
    11821 cgcagatggc ccccgtcatt cactttggtc cctgggaaca agtgacgccg caggatgtgg
    11881 aggtctcgga gcgacatttg cagcgactgc gctccgggat tttgctgacc gataaagttt
    11941 tgctggaagc cgcgcacggg cgcatcatct cgaccgataa tattgctctg gcaaaaattg
    12001 ccttcggcgc aaactcaccc cccggaccga tcattggcga tgtgatcaat gttaatagtc
    12061 ctttgcgcta tgacaaacga atgttgggcg gactcatcac ctacgcccga gcaggccaag
    12121 tgacaatgat cacgccattc atcctggcgg gcgccaccag tccgatcacg atggcagcgg
    12181 cgcttgccca acaaaatgca gagacgctgg caggagtcgc attgacacag ttggtcaacc
    12241 ctggcgctcc cgttttgtat ggtggcttca ccgtcgatat ggacatgcgc tcaggaaacc
    12301 cggcgtttgg cactcccact ggcgcatggg cagcgctggc aggtgcacaa ttggcgcggt
    12361 actataaact tccatatcgg ggcggtggcg gtctgacaaa ttcccatttg cccgacgcgc
    12421 aagccgtcca ggaatcgcag tggatgttgt ggtccgcgat catgggtcac gcgcatgtgc
    12481 tcatgcactc tgcgggctgg ctcgaggcgg gacttaccgc ttcgttcgaa aaatttatcc
    12541 tggatgtgga aagccttgcc atcttccagg acttttttca cggcttcgag atcaacactg
    12601 agacgctggc gttggatatg atcgcgcagg tcggtcccgg tgggcacaac cttggtacgc
    12661 cgcacaccat gaaacgcttc cgtcatgcct tttacgaatc caccatcaat acgcgccaga
    12721 ataacaaggc gtggatggat gcaggcggac tcgatgccac ccagcgcgcc tacgtgattt
    12781 ggaaacaatg gctaatggaa tacaccccgc cgccgcttga tcccgctatt cgcgaagagg
    12841 tggaagctta tgtcgaacgc cgttcaatcg agttgaatgg cgtggaactc tatagtgatt
    12901 agagaaagtt tggcaaggaa atgaaacaca ggaggattgc ctcctgtttg gatgtatcag
    12961 gctagcacac gctgttttgg cttgtactta acatctccag gcttcagcca gcggtctcga
    13021 aatctgtgtc cgctggcctt tgccgagtgc ttgacggaat tttcccgaac agcgcgaatg
    13081 gattgggttg ccgcttgcat aattggggcg atattttcag ttgtttgctc ggtggagatg
    13141 gttttgtctg gcacgcggga atgacatcga tgttcgtttc gtcaggaaat cgaagggtgg
    13201 agttaattga tccctgatga ataaagtgtt tcttgaaact tttattcata cctaatatct
    13261 aactgaataa ctttgatgga tataatcgat tctcgactga gaaacgggaa tcatttcaat
    13321 agattctatc caaaatatgg aggcactgaa attggcagca ctggaactga acaaacagga
    13381 aataccggga aggatagtgc tggaaaactg tttattgaac ttgaggatgt ttgttaccag
    13441 gcagtttgaa gtcggtgacc gcatcgctgt gcgagaggcg ttggtgtacg agtgagtcct
    13501 ataaggcaat tatgactttg tatggctccg ataatccatg ccttttacat cactgtcaat
    13561 gaacatggag gcgatggtga catgtcgcgg tcgtgtaatt gcgtagatga ccgcttccag
    13621 gatatcgcgg ttggttacca tctctgcctt ggtccgggat gaaacagtac tccactcttc
    13681 atcgagaggt gaaatatcct gaatccaggg tgggtgaata caaatggagc ggattggcgt
    13741 gcctaagaac atttggcgca gaccatcagc tagcgcggtt tgaccatgtt ttgcagcata
    13801 aaagggaact gaagtgctat aaagatttac attggtcaat ccgctgacag agccaatgtt
    13861 gactacatcg gggcgggcgg atttttgcag ggcggggatg agcgcctgtg taaaaagaaa
    13921 cgtgcccgtg atcgccgaat taatggtatt catcacttcc gtggcttcat aaggcgcatc
    13981 ccgctgaccc agccacgcgg ctccattgtt gatcaaaatg tcgatacttt ggaaatccgt
    14041 gttgatttct ttcgccgctt cttccacacg ctcagcattt gcaagatcta tctcataact
    14101 tcgaggtcgg cgttttatcc tggcttcgat atgacctgcc gtgactctta atgcatcaag
    14161 cgaccgccca cataagatga cgttgcagcc taaatcagat aatcctaacg cgatcgccgc
    14221 accgagtcca cgcgccgcgc cggtaatgat tacgttgaca ttctccagat ttgttatttg
    14281 gtcctgcata gctacctttc tgtaatatcc gaattttagc cgcgtactaa aaatctatga
    14341 ggaatataat ctagtcatca tttccattcc gtaaaatatt ctgaggtgtt gctatgggaa
    14401 ctcgaaccca cgacgtgata aaagggcgtg ccaagtatgt gctgatcctg atcgttctgc
    14461 tcaatatgct ctatcccatc accggcagtg ggagcaccat ttccttgatc gcctttgcag
    14521 cattgtatgc agggttattc atcgtaggta tttttattac cagtgacagc cgcgctcaaa
    14581 ttacgtggtc aattagcgtc gcggtgattt ggttggtcat gaccgtcgtc tgggcattgg
    14641 acccaaccag tttctggaaa actcaagtct cttcatttat tctgattgtt tttcacagta
    14701 cgatcatact cgtattgatg cgctatatct tcacggcgga acaagtcaca gcggatgtta
    14761 tctacgcagc gtgcgctgtg taccttttgc tttcgttctt ttttgttcca gtctacggaa
    14821 tgttggaggc tgcgctcccc ggttcctttg tggataatac gcttggttca cccgtgcact
    14881 ggcagcagtt tgtctattac agcctggtca cgttgagcac tgccggctac ggggatatcc
    14941 tgcctgcgaa tatgtgggca cgcatgttgg ctgggctgga agcaacgatc ggtgtgttat
    15001 atgttgccat ccttgtagca cgattggtta gtctctacga ggtcaggcgg cgacagtgac
    15061 gcagctggag aaaaatttac aggtgttcta ccttagccca agaccaggaa aattccttac
    15121 agttagcggc cggtgcaggc gcattttttg agaaaattag ctacagtctg gatagatcaa
    15181 aatggacaat caaaatacag ctacaaattt gcaatcaaag aaatggcgag gatcgcttat
    15241 tcaattgctg cgcttattgg gaggtgctat ccttggatac ctcattagtt ttgctcttct
    15301 tattttcgat ggaacagcct ttttgccgct tcttggcaag ataattcatg gccaggaaga
    15361 attatttgag ctcctctttt tttatgcatt gtatgactcg ttacgttcaa tgggtgcagt
    15421 tcatggtatg gcagaatttg tttacacttc tctttgggca ttgattggag ctttactgat
    15481 gtcgggtaga agaaaacaaa taaggatcgg agtaattttt cttatcctat atgtgattgt
    15541 cgggtgccta gcctatagta ttttagtgat gttaagggtc ccaacatagt ttttgaaata
    15601 cattcacgta gaaaagaacc aaacaaacaa aaccactgag gaggatgtaa tgttgtgagg
    15661 cgagctaagg tagagcttat atctcattta cagcatgcgg aggtcgttcg cctcgcaacg
    15721 cgtatgcaat gtcacccgct gcccattccc aaacggctgc atctgcttct tcggagcacc
    15781 aggcggagtg tggcgtgatg aagaaacgat catcatgcag aaaaggatgc tccggtgacg
    15841 gcggctcgac ggataaaaca tccaatgccg ctccggcgat tctgtcgttt tggactgcat
    15901 ccagcaacgc ctcttcctgg atgagcaacc ctcgcgcggt attaattaga taagcagaag
    15961 gtcgcatcaa agaaagcaaa tgctcatcaa tcatgtgatg agtctcttct gtaagcggcg
    16021 tatgcagagt cacataatct gattcacgca tcagggtttc tttctcaaca ggtttcacac
    16081 ctgcatccag catttgcttc gggtccacat acggatcgtt tgcgatcacc tggaatccga
    16141 gtgccattgc cttggctgag acggatctgc caatgcgacc ccagccaatg atccccaacg
    16201 tttgattgcg tggatgatgc atcggacgca ccggacgcgg gtcccattca ttggcgcgca
    16261 ccagttgcct cacctgggga agtcgccgca cctgtgaaag caagagcgcg atcgtatgcg
    16321 tggcaacttc atccgtgcag tagccggggc aattcgtcac ccagatgccg cgcttcccgg
    16381 cgctggcgtg atccacgtaa tcataccccg tgctggcaca agcgatgatt cgacagccac
    16441 taaaggtttc cattatgacg tcagtcaccg ggtgcatttc ggtgatgatc gcatctgctt
    16501 cacgatagag agatgcttgc cgctcatcgt gaaaaccaat gacgcgatgc actgccacat
    16561 ccagaccagc aagcgcgttt aatgcttcag gtgggttacg agtatcaatg caggcaatga
    16621 caatattcca ggtcatgatt tttctccatg gtttgggtag gatatcctga ctttgtttga
    16681 tccagaatta tatgtctcaa ttcacaaagg acaagaacga attaagggat taagagaaaa
    16741 ctgcggagca tgaaagcact ccgcagtttt tatagctatt gatcgtttcg ttttatctat
    16801 tacggaacat atcgtacctg aacactgatc cagtcgagcg agaagtcgcg agcggtattg
    16861 ctggcaacgc ttgtcaggcg aacacggaag ttcgcgtttg tgaagtcgcc acttgaccag
    16921 gtgcgtcccc acgtatctgc cagaccacca agcagatagg tggcttcact ggtcgtcagt
    16981 gtagatgtgg tttttgcagc cgtccaggat gcgccaccgt tccatgagat ttccacacag
    17041 aatcttgggg ctcctgaagt gctatccgct cgcccatccg tgcggacttc gatacccgta
    17101 atggcggcgc cgctcgggat gctgaagtta tagttataga aggtatgcct gtccttgcgg
    17161 ttgctggtgc aggatgtgtt gttgtttgaa ccgctattgt tatccacggc aaataaaccg
    17221 tcatttgcca acgcgtttcc tggattaact tcaaagccgt tattgtcgcc cgaaccactg
    17281 gtcacagccg cgtttgccga tgggctgagc tgacctgtat tcacgggtgg gttgccacta
    17341 ccgcaataca acgatccctt gccgatcaca tcatacgggc aatctgcctg ctccagcagg
    17401 tacagcactg ccgcgcggtt gcgagatgtt tgggtaggga tcacttcatc cggcgggtag
    17461 aagccacctt gcgaggaggt gcgcggatac atctcgaacg tgtaattcaa aacgccatgc
    17521 acaccataca gccaatcgtt gatggtgccg tctgtgatgt acaggtcgct ggcctgttcg
    17581 ggtgtatatc cattcgtggc ggccatggcc tgtcccatcg caaccaaaac gtcgtggtca
    17641 tcctgtgtca tgtcggctgg cacatcggtg aatgtgtagc catacggcca gaggactaac
    17701 tcactataag tatggaaatc aatggcgacc gtgatctgtt gttgaccacc gatcacgcgt
    17761 gagttgacga agtcgcgtac tcgttgtgtt tctggcgcag aaaacggcgc ggagccacga
    17821 tacgtttcac tgctggtcgt tccgctggat ccaccgcagc atccccaacg ataatcccag
    17881 ttgcggttga ggtcagtgcc aacggcagag gaaccggcat tcggctggcg gttcttgcgc
    17941 caggatctgt agcttcccgt ggcaatgtca tattcgccgc catcgggatt catgtcgaag
    18001 accatccaga tttcacggcc attcaccaga tcggtgatct gctgggtgct gccatattca
    18061 tttgtgagaa tcccaagcgt gtaaagcgcc atttccacgg tgaggtgctc acgagcatgt
    18121 tgatgatggg tgaagagcac ttcaggttcg gcttcgtctg tgccaacgtt atctgagatc
    18181 ttgcctgccc aaatggtgcg tccttcgtag gaggtaccga ggctgaacag gtcaaagatt
    18241 gcaccgtgat ccaactctgc ctgttggatt tccgcgacca tttcagcgta atcatgatac
    18301 gccgagtcgg caggcgggaa tgccagaggg agggcttgtt ccagcgtcgg tttggcaatg
    18361 gtcttccccg tctgtcgttc aatggccctt ttttcctgtg cagttgcttc caccagcaca
    18421 taatcgtgac ccacttccag aatcaacgcg ccagatgcgg caatcgccga gcgcgtctcg
    18481 gcgtcaaata cattggagat ataaaagacc tctgtgtgat ctgaaggtcc ctgtgcggcc
    18541 gctggaggtg aaaaaccaaa catcattacg atcaggatta acccatgtag ccagtatttg
    18601 cccttaacca tacaaaactc ctttttttag atggaaactt ttgatatgct tgctctgatc
    18661 tctactaatg atgtgtcttt agtaattctg gcggataacc tccttgttct atcggaagaa
    18721 attaatccat tggatctcaa aagaattccc ggctcttcga atgaattcat ttgggattgt
    18781 gatctcgaaa tatccttcac caagaggaat ttccttactc ccctgacccg aaacgatctc
    18841 cacttttgtc cataaaggat cgattggggt aatgacatat tcattccccg ccgcgagcac
    18901 cgattgtccg gtgaggttga acccttcgct gctcgaaccg gaggcggtga gcgtagtttg
    18961 ttcggatacc agcttgaact gctctaggcc cgtcaggtgg agtcgcaaaa cgattttctc
    19021 tggcattgcg cctgactcaa gttgcaatgt cgcggaaccg atcccggtcg ggctatggat
    19081 atcaacggta gttatgctgt ttttgtgttg gatatcgatc tgatcatcct gatttctggt
    19141 agtgacgttg aacaccggct cacctcctgc ttgcgcgttt gcaggctgac tcgcacagga
    19201 gataaggaaa acactgaata gaattgtatg tacaacgaaa tatttcatct tggttgaata
    19261 tcaaggggtg agaatgtatg aatagtatag cacgcctgtc aagtcttcag ataggaacat
    19321 gttacatccc tgattctcaa acgtatctct atatattgca tcccaatata aaagcctcat
    19381 ggagcaaact gatcaactct tcatccaaat cgtcgaagcg gactagccga aaaatatgcc
    19441 cgtaatctcg aaagactccc atctccagct tgtggatacg tggatgcaca gcttgtcgct
    19501 gcagccatag atacccttcc agccagcgct tgcgcggcac cgctgcagcg aattgcacct
    19561 ccgcctggaa gacgattcgc gtcttcaccg ggaatgcttt tactggaccg aacagttgca
    19621 atttttcaaa caggagatca tataactcat ggaccagggc ttcttttcca gcaaagtgat
    19681 cttctaagct gtacagtcca caagagtgat tgcgtttgac tgaaacaaat tgccgtccac
    19741 aggttgggca gacccagagt tgttttttag gagtcattta tcccacgctt ggttttcgtt
    19801 tttccaattc attgattaat tcgtagcggt ccttttcgtt tttctgcatc accacgacct
    19861 ggcaattcct gggattgcgt aaattttcaa attcctccac cgtccaatgg aaccaacgca
    19921 tcaggaacaa acggagggtg agtccatgag tcacgatcag ggtgttttgg gcatagctgg
    19981 gttttttgaa gtcgcggtgc aaggtttcca taaaggtact gactcgatca taaacatccg
    20041 cacccgattc accatcagga atcctgtaat aaaaagtgct aaagccatcc ctttgcctac
    20101 ggatttcttc attttcatcc ggatgccgca gatgccccca atcctgttcc ctgatgcgtg
    20161 ggtcctccac aaccttgtca atatttgctt caagactgat tctcagatgc tgaaacgtct
    20221 ggcgggttct cacatagggt gataggtaga cgtgaagact ctcggcgccg atgatgtctt
    20281 ttatctggac ccctgccttt gtggattgct cgatcccttt ttgagtcagg agtagggcat
    20341 aatccggaac ggtctggtat tgatttgcat caatgttgcc ctgagattgt ccgtgccgga
    20401 tcaggataat gcgatttggc ttcatgagac aggaacccta taaattaaaa cccgctttgt
    20461 ctttcaatgc cttcggtaat atcggtcgtt tgcttcgctc gatcaaaaac gtagccactt
    20521 cagtgatctc gcgaaacgta tgatgctggc tcaatgtccc ttcgagatag cctgcgccgc
    20581 tggtgccgcc ggtcccgatg cggatgccga tcatcctgcg taccatcatg aagtgcctgt
    20641 aacgccagtt ggagagtagt tcgtcgatct cggagagggt gttcaacaat tcaaatggga
    20701 attggaagat aggcaaatcg cgatagatgg tgatgaacaa ggcggctcgc atagccgccg
    20761 gggaaatttc gcctctgcct ttttcgaaga atacatcatc aaactcctgt aatcgaccag
    20821 actcattttt cgatagactc ttttggtaag catttcgata atccagccag aatttatgtc
    20881 tatccttaaa gtctgactga gacgcttgtt gataatcact ccagtaacgt tcttccagat
    20941 atggcatccg ttccagccac tggatgatta actctttcag tgtactttga ctttctgcct
    21001 ggtttacttc ctgcagatcc gcttcggaga gactgccctg acgagaggct ttgtaatatt
    21061 tggacttatg tctttgttcc attttcaagc ctaattttgc ttcaattagc ctgaactgtt
    21121 tgctctgaaa ccccgaagcg ggcagcaaga catttctaaa ttccaaaaag tcgagcgcag
    21181 tcattgtctc cagtatattt acttgctggt tgatcaattc cagaatcttc ccgatccttt
    21241 tcattttata cacaacattg ctcatgtcgg aagagttgtc attaatatcg ccccggatga
    21301 agatgttcct tgccaggtct agttcaaaga tgatctgttt gaaccatagt tcataggcct
    21361 ggtggatgat gatgaacaac atctcgtcgt tcccatcttc catggactca aagcttctgg
    21421 gatgctgact gttcaggatt ttatcgagtt cgatgtactc agaataatag aggtcttcca
    21481 taaaatatct ccttctttat cttgtcatta cgagcctccg ccggtcgagt gccgccacgc
    21541 ggtgtatcga gaccagcgag gcaaagattt tgaggagatt gcttcgtcgt cgctttgctc
    21601 ctcctcgcac ctccatgaca cggcgagtat agcacgcacg cctctcaggt ataatttggc
    21661 ggctatgaca agcgaagcag tctcgaggac gaacggagca gccctgtggg tcaaagccct
    21721 gatcttcgat ttcgacggac tcatccttga taccgaaacc ccggattttc aagtttggca
    21781 aacaatttat caggaacatg gcttcgaact gcctcatgag gaatggggga agattgtcgg
    21841 tggatttgga ctggcgcact ttgatgcagc ggaacacctg acctctctcg cggataggga
    21901 gctggacgcc gtttctctgc gtgaccgtca ccgctctgag agtcacgcgt taacacttga
    21961 gcagccaatt ctgcctggtg tgctgaacta tattcaggat gcgaaacgac ttgggctcaa
    22021 gctcgcaatt gcatccagtt cagagcattc gtgggtggac acgcatgcga aacgtcttgg
    22081 gatattcgat cactttgacg cagttatctg cgcagatgat gtgtcacctg gccggaccaa
    22141 gccgaaccct gacctgtacc tgttggcgct gaatcggcta aacgtccaga atcatgaggc
    22201 gattgtcctt gaagattctc ccaatggggt gcaggctgcc aatcgggctg gaatttttgt
    22261 tatggcaatc ccgaatccgg tcacatcctt actaaagatc gagggggcga atctggtatt
    22321 caagtctctt gcagatttgt cgttgcctga attgcttgat aaagtaaaat aaaaacattc
    22381 aattggagga gcacgatgac tacagaaaaa tttcgctggc aggggatcgt ttcgaaatat
    22441 gcgtaccctg aaacctggcg cagttcgtgg caggtggcta atacacttat cccgtttatt
    22501 gtgggatggt acttgatgta ccgcagtctc gagattgggt attggctgac attgatcctg
    22561 gctgtaccca cagcaggctt catgatgaga ctatttatca tctttcatga ttgctgccat
    22621 gggtcctttt tcaaaagtat gaaagccaat gaccgcctcg ggcttgtact cggtgtgctg
    22681 gtatttaccc cgttttacga gtggaaacac agccatgcca ttcatcacgc aacggcaggc
    22741 gacctggacc ggcgcggcat cggtgacgtg tataccatga cggtggagga atatctagcc
    22801 gcaccatggt acaaaaagtt tggttatcgc atcatgcgca atcccctgat cctgtttacg
    22861 gtaggttcat tcattgtgtt tacggtgacc catcgctttt gggagagggg ctcgggcaaa
    22921 cgcgagcgaa gcagcgtgat ctggacgaat atcgctctgg cagcctttat tggctggatc
    22981 gtcattgaaa ttggctgggt tgctttcctg ctggttgaaa ttccggtttt gttgctggcc
    23041 tgcggcgcgg gcgtctggct gttttatgtg caacataatt ttgagcccac ttattgggaa
    23101 cgccacacag actgggaatt cttcaatgcc ggtatggatg gcagctcttt ctataagctc
    23161 ccgaaagtgc tgcaatggtt cacgggcaat atcggttttc atcacattca tcacctcagc
    23221 ccgagaatcc cgaactataa acttgaggaa tgtcataatg aaaaccccgt gttccagatt
    23281 gagccgctga cgtttggcgc cagcctaaaa tctcttttct tccgtttgtg ggatgaaaag
    23341 gaaaaaatgc tggtaggctg ggatgccctc aaccgctacc gtcaaaaagc ggacgcttcg
    23401 tagaaacgag aaggagttgt tcctggaaca actccttctt tatttgatgg gttcgcgatg
    23461 gaagaattag atcagatggc ttctgcagcc agttctttct gccgcagtgg agcactcacc
    23521 acgtgcaggt attcaagaat ttccacatac gaatggaaca tgctggtctc gtgatagctg
    23581 atttccaggt cgtcgcaaaa tttgcgaatg atcgtctgag cctcgcgaag ctgattccgt
    23641 gccagcgagg ggaagaggtg gtgttcgatc tggtaattca atccaccata ccagaagtcg
    23701 gtgatgggat gtgctttgac gttccgggca gtaagcactt gcaggcggag gaaatccatg
    23761 gtttcattct tgccgaccac cagcattcct ttatggttag gggcaaacac agatgccata
    23821 tatagaccaa atagagcttg ctggaccagg ataaaaacta tccccttcca tgcgccaaga
    23881 gcaaggaata tcaggctaaa aaaccatacg aaatggagag ttaacaacag gatctcaacc
    23941 aggcgatatt tccacgtcgt ccgttgaatg aagaaacgaa tactgccgac gtgcaggctg
    24001 tacgcttcga agagtaaaag cgggaagaaa aagaagctct ggtatttgac gataaagcgg
    24061 ggaatgccgc gtttttccag agcggctttt tcggtgaagg cgatcaccgg aatagcgata
    24121 tcggggtcca tgtcttcctg gtttgggtgt gcatgatgtt ggttatgttt ttccagccac
    24181 cagccataac tcatgccgat cagcaggttc gcatgcagga tgccgaagaa gtcattttgt
    24241 tttgtggtgc ggaatccctg gcgatgcccg gtatcatggg cgataaagcc catttgccca
    24301 aagacaaacg ccatatagac cgcgttcaaa atttgaatcc aaaggttatc cgttagaaag
    24361 aggatggcta tgctgactgc caacaaaccg gcagagagaa ggattttgta ggtaaggaag
    24421 gttggctgct tctcaagcag tcctttttcg aggatcaggc gtttgagagc ggcataattt
    24481 tggttatcgg ggacagcagg attggcggtc gagtccggtg tagacattga ttctcctccg
    24541 gtgatggatt acacaaaatg cattctttgt gtggataggc tggggttgcg actgaattgt
    24601 aacagcatct tcgatgcgat gtatgacggg tccctccatc cgctttataa tttggactat
    24661 ggatgaccgg gaattactgg agaaatatga accagtgctg cgttttgcga agagtgagcg
    24721 attcttcccg atggcagtag agccatatat cgagaaatgt acaatcttcc cgagcggacc
    24781 gcagggtgtg atcggcctgt tgtctcatct taatgagccg ctggtcaagc ggctcggcaa
    24841 attggagagc ggggaatact ttctgcgttt cgtcaatgac ccgctcattg attctgatat
    24901 ttgggtgtgg tggggtgttt tgtcgtcggc ggctgctgtg ggaggcgggt ttctccgagg
    24961 ctgggatggg gttgtgggcg ctgcgatgtt tgccctgtta gctgccttcg tcgttttcat
    25021 tcaggcttcg cccatccgtt tgcgcttttt tctggcggca tccgccgcaa tcattttctt
    25081 tgtgcttgga gccctgccgg tctggttttt tctgaccccg catgtgttta tcagtgtagc
    25141 gattgaatat cttatcttac tgccaattta tttattggtg ctgttctttc ttacaatacg
    25201 gacaatgaaa tttatctttg actatatcat cccggaagcg ccgggagtga ttatggacat
    25261 gctgtctcgc tccaccgaga ctgtggcgcg taagtcattt tggcaatatg cggaaatatt
    25321 gaagaaccat ccagagcctg tttactacgg acgcgtggca gcttggcagg atgtggaagg
    25381 caatcaatgg aagagccttc aatatcattt cttttacgcc tttaatgact ggcgattggc
    25441 ggccaatggc atcaatcacc atgaaggcga ctgggagatg gttgccgtct atctaaaaaa
    25501 taatgagccg cattccttgt tgtgttctca gcacgggacc ggcgcaatgg agttatggga
    25561 aaatgttcgc cgtgtgaaaa atatggatgg gactgagaca atgcatcctc tcatttatgt
    25621 tgctcttggc tcacatgcca attacacccg gccgcaggtg atccgctcgg cgagcctttt
    25681 tcatgaggga ttgttccagc gtttcattta ctggactgac ggcctgatcc attttctatt
    25741 catgctcttc aaccccagtc agaaagcacg ccagatcgca ctgaaccaat tggcgacaca
    25801 tcccgccatg gcgctgaccg aagagtcgtt tgaaaatctg cgtgatgaga aggatcatta
    25861 cctggtgagc ctgccgatgg agatcgcaac aggagatggg ttacgtatcg gctatgcagg
    25921 cgatactaga ggggagaaga tcggtatttc aagcagttat ctgaagcgtg ttatgtcgaa
    25981 tcggaaggtt gtgcatcctg gattaaaaga atggaaacag gtccttttat ctcccgagcc
    26041 ggactgggtg caatacaaag gcctgtgggg cgtgaaaagc ctgatgaagg atgaatcggg
    26101 tccaccggga ccaaaatggg accggcctga taagttattc gctgtctacc cgcgcaaacg
    26161 ctgggatcaa gcgctggaat ggttgacaga gttggaaaat atcttgatgt cgaaaaataa
    26221 aaagaaaact tagccttcga ctttattttc ccaccgcgca tacactgcct gcgacaataa
    26281 ccttcccgcg ctttgctcgc gcgtgaccag ctctcccatt tcaatttttc ccccgaagcg
    26341 gctcatcata tcttccatcg cctgtgccac atggatggct gaagcgcgca ctgcatagag
    26401 agttgtcaca acaaataagg gcttatcact taaacacgcg cggcacgcct cgaatagact
    26461 aggcagcgat ttatacactt cccacacttc gcctttgggt ccgcgcccaa acttgggagg
    26521 atcaagaata attccatcat atttgacccc acgccgcgct tcccgctgaa cgaacttcaa
    26581 cgcgtcgtcc acgatccagc ggataggttt gtctgttaga tccgataatg cctggttctc
    26641 ccgcgcccag cttacagatt ttttcgaggc gtccacatga gtcactttgg ctccggcaga
    26701 ggcagccgcg agggtggcga gacctgtgta tccaaataaa ttcagaatat ttacgtcgtg
    26761 acctttgtgt ccttcgtgtt gaatcaaact actgaaccag tcccagtgcg cggcaacttc
    26821 agggaacacg ccgagatgac gacctggtgt tgtcatggcc cagaacttaa gttctcctct
    26881 ccctatggga gagggggtag gggtgacgga tggatcaaga acataccgca tctcccacct
    26941 ctcttccacc tttttcttga aatcccaatg tccgccgctt tcttcccctg ttggctgaaa
    27001 gaccgcgtgt gcattcttcc attcattatt cagtgagcgc ttccacatgg cttgcgattc
    27061 aggccgcaca aaaacatagt taccaaaacg ttccagcttt aatccatcac cgctatcgag
    27121 cagtgcataa tctgtccaat gcgatgcttc aagaagagtg aatgatgatg aagtcataaa
    27181 gaataggaca ttggatgggg aagggaatta gttacaacta cttgattttt cgtctaaaag
    27241 ctgtataatg tgggaaagtg gtagaaagtg gtacaaagtg gtaggacgcc ggttgcgccg
    27301 gcgttcgtta gttaatagga attatagcac aaatgttctt aggccagttc cagcacaatc
    27361 tcgatgataa agggcgcttg atgatcccgg cgcgttttcg tgaattgctg gcagctggtg
    27421 cttttattac acaagggttt gatcgttgtc tcatggtgat gacggataca tacttcagac
    27481 aggtctatga acgtatcagc gcaatgaacc tcgcggaccc gatggcacga ctcttgcgca
    27541 gattgatttt atcaaacgcc tatcctgttg aagtcgataa agttgggcgc atcctccttc
    27601 ctcagaattt acgtcagagc atcctgctcg aaagcgaagc tatcctggcg gggcagggcg
    27661 aatactttga agtgtggaat cctgccgaat ggaatgcgca aatggcacaa ctgcaggata
    27721 tcgagacgaa caatcagcgt tttgcggctc tcgatcttgc taactatagc ggctcgtagt
    27781 gacgacttta gtcgtcgaca taacgactaa agtcgttact accagatgaa cgagtcgccg
    27841 caccaacctg tactttacaa agaaattata cacgcgttgc aaccttatgc cggtggtcgc
    27901 tatgtggatg ggacattggg cgcgggtggt catgcccgcg gaattttgga ggcctctgtg
    27961 ccagatgggc aactgctggg tctggatgtg gaccctcagg cgctggcgct cgctcgcaag
    28021 atcttggctc cttacgaaga gcgcgttcat ctggcacaag cctcctatgt ttctctgtcc
    28081 gatcagcttg cgcagttagg ctgggcagca gtggatggca tcctccttga ccttggtgcc
    28141 tcttcgatgc agttcgatac accagaacga ggcttttcct tcgctcacga tgctcccctc
    28201 gatatgcgct tcggaccatc catgctgaag accgccgcag atctggtgaa caagtacgac
    28261 gaacgagaac tggcagactt gatttacaaa tacggcgaag aacgggagtc gcgcaagatt
    28321 gctcgagcca ttgtcaaagc cagaccgctt catacgaccc gcgaactggt tgccgttatc
    28381 gaaacagtct ctccccgacg aaggtccttc gacaggctca ggagcatcca ccccgcgacg
    28441 agaaccttcc aggcgctgcg gatcgctgta aatgaggaat tattatccat tgagaacgta
    28501 cttcctcaag cagtggcaag cctggggtcc ggtggaagac tggcagtgat ttccttccac
    28561 tcgctggaag accgtattgt caaagattac ttccgtgaac aaagcaagga tctggtcaac
    28621 ccgccttatg aacagatcta caaagaagag cgcaaagcaa cgctgaaaga ggtaaatcga
    28681 aaaccgatca cgccctcgga tgaagaaata aaaagtaacc cgagagcacg cagtgcgaaa
    28741 cttcgcattg tagaaaaatt gtaaggtaag gaacggcggc tcgcgaagca ccctgatgag
    28801 ggtgccgttc cctacacgac caatcgccca aatataaagg tcgttagtta tgcaggctgc
    28861 acaaattatc catgctcata aacaggctcc ctggcgtgtt caacgccagt gggttggcgc
    28921 gttcctgctt gccgtcatcg gcatggcaat gatcgccgcg ctctatcttg atgtcactgc
    28981 ccgcgccgca gtgaccgggc gcgaaattca ggaactgaga attgaaatca caacgatcca
    29041 gcgtaccaac gcggatctcg aaacgcggct ggcaaatatg acttccacag ttgaaatgca
    29101 gcgccgtgcg ctcgcattgg gctatcgccc tgtgaagccc ggcgaactgg actatgtggc
    29161 cgtgcccggg tttaccgtcc ctgagcctga gattttgctg gtcgctgatg atgcaccacc
    29221 tccgacgcaa tcgttacccg ctgagttttc ccaatccctg atcgaatggt tcgatgagcg
    29281 cattcgcttt ggaggcgctc catgagacaa caatacgcca tccgttccct gattcttgta
    29341 gcatgtcttg cgtttgtagg agtggcggtc attgtccaaa tggttcgcat tcagaacagc
    29401 gaacaggcgc agatcttcct ttcacaggga gatcgctacg cgggcgaatt ccacatgttc
    29461 taccccgagc gcggcgagat ttatgatcgt cacgggcatt tgctcgcggg caaccggacc
    29521 gtgtatgagg tgggggtaag cttaagcgag atggaaagcc cggagtccat ggcgagtatt
    29581 ctcagttcct atctcggact cgcctttgaa gatgtctatg acaagcttac caattctccg
    29641 gaaacctggg aatacattgt ggtccaggat tacgtcggtg cggaaacggt cacagccatt
    29701 caagccctca tgcaacaatt tgatgagacg gatgatcagc gtttaagcgg cttgggattc
    29761 aaacctcatt tccagcgtag ttatcctgag acagccctgg cgtcgaatgt attggggttt
    29821 gtcacccgcg atgggcgcgg ttattttgga atcgaagaaa aatacaatga tctgttggca
    29881 gggaatcccg tccaggtgtg ggtgccgagt gacccgaaca aagccctgga aatccccaga
    29941 gtgccaaatg gcacgacgct tgttcttacg ctaaatcgtg acctgcaatc cagcgttgag
    30001 acaattctgg atgatgccct gacagaatac ggggcagaga acggcaccat catcgtcatg
    30061 gaccctcgta acggtgaaat tctcgcgatg gcagcgacgc cgcgcatgaa tctgaatgat
    30121 tacagtaatt acttctcgct ttacgatgat ggcagtcaat ataaccgctc gattggcatg
    30181 gcctatgagc cgggctcggt catcaaagtt cttacgatgg ctgctgcgct cgatgcgggt
    30241 accgttcagc gggaaactcc attcattgat accggcgcga ttcaagttgg tggtattacc
    30301 attcaaaatt ggaaccgcga tgcctggggg cagcaaaaca tggtcggatg tctgcagcat
    30361 tctttgaacg tctgcatggc atggatttct tcgcaaatgg gttcgcaatc cttctatggc
    30421 tacatggagc gctttggtct gggtcatccc accggcgtcg acctggctgg cgaagcggtc
    30481 ggcaggctca aggtcccggg cgatacggat tggtatcccg ttgaccttgg caccaatgcc
    30541 tttggccagg gcatttcagt gactccgtta caaatgttga tggcggtctc tgccattgca
    30601 aacgatggac atatggtaac gccgcatgtg ttgtattcga tggtgcgcga tggtcatcaa
    30661 tataacgttc cttcgcaata tgcaggctca ccgatctcgt cggaaacagc caatacattg
    30721 aacgaaatgc tggcagtttc ccttgagcag gaatcttcgc aggcactctt agccggatat
    30781 cgcctggcag gcaagacggg taccgcgcaa atccccaccg attttggtta tgacgcgttc
    30841 catacaaacg tgtctttcat cggctggggt ccggtggatg agccgcaatt tatgatttat
    30901 gtctggctgc aaaaaccaag ttcgtccatc tggagttctg aaacgaccgc tcctgttttc
    30961 tcgcaggtgg cggaacaaac tgtgattcaa ctcaacattc caccagatct tgttcgaaat
    31021 cagttatcta tcaaataaag gtaaacgttc ttattttgtt tttcaggttg atttgttcca
    31081 acgactatgc tgactctcgc cgatgcaatc gaagccctta caagtttccg tccactggct
    31141 gtcacagtga tcacagaagc agtgattgac tcgcgtcagg tgatccccgg ctcgctgttc
    31201 gtcgcgattc ccggtgaaca ggtgaatgga cacgacttta tcggggatgc ctttaagcgg
    31261 ggggcatctt ttgctttaat ccagaaggaa gttgatgctt cgtttcggac tgtggacctg
    31321 cgttccgttc tatcagcaga gtctctctcc gaacgggatt tcactactcc gttgtgcctg
    31381 cgcgtggaga acaccgttac cgcgttgcaa cagatcgcgc gttactggcg gcgcaagctg
    31441 gacctgcgtg tggtgggtat tacaggcagc gtagggaaat ccactaccaa agaaatggcg
    31501 accgaagtgt tgagcacgcg ctatcggacg ttgaaaagcc ctggtaatct taacaatgag
    31561 atcggactgc cgctcacaat attgaaatta agctctgggc atcagcgtgc cgtattggaa
    31621 atgggatttt atgttccggg cgagatcgct tttctttgtg atattgctct tccccaagtc
    31681 ggcgttgtga cgaatattgg cacagtccat gccgaacgtg caggttcaca ggaagcgatc
    31741 gcacgcggca aagccgaatt ggtccaggcg ttacctccag gccccgaagg cattgcaatt
    31801 ttgaacttcg acgacccgtg ggtccgcaag atggaagaaa agaccaaagc gcgcgtcttc
    31861 ttttatggct tgtctcctga agcgcatctc tgggcagatc aggttgttgg tcttggcctg
    31921 gatggtattc ggttccgtct tcattacgcc ggagagacgt tgcacgtaaa aataccattg
    31981 atcgggcgtc actcggtgca cacggcattg cgcgcggccg cggttgggct ggcagaagga
    32041 atgaactggc aggagatttt agaaggtctc atgcagggac atactcaact gcgcctcgct
    32101 gcggttcgaa gcgaaactgg cgcgttatta ctggatgata cctacaatgc ttcacccgaa
    32161 tcgatgctgg ctgcgctcaa tcttctcgat gagttggatg ggcgaaaggt agcagttctg
    32221 ggtgatatgc ttgaactcgg tccgtatgag cggggtggac acgagatggt cggtttgcgc
    32281 gccgcgcaag tggcaaacat actgctcacg ttgggcgaac gtgcgcatat ttttggcgaa
    32341 gcggcgcggc gcgcaggcat gaagaaatcc tccattattg aatttgatga ttttgatccc
    32401 ctggtcgaat ggctgaaatc aaatctcaaa aaggatgatg ctgtgctgat caaaggctcg
    32461 catggtctgc gcatggatcg tatcaccagt ctgttggagg tgcgttcgtg agcatgaatc
    32521 atactgccct ggtgttggga ttggcgatgc tctcttttgt gatgacggtg atctggggca
    32581 cgccgctgct gcgtgttctt cgtcactacc gcgttggcaa agtcatccgt gtggaagagc
    32641 ctgatcggca tcgtgtgaaa atggggacgc cgaccatggg cggcgtgttg ttcatcctcc
    32701 cagcaatttt gctgacaggt ttgttaaatg ctgcggcctt gatcggcttg gatgtaaccg
    32761 gccagtccgt ccttgtgccg ctcatcgttt tggtctccta cgccgtgctc ggtgccctgg
    32821 atgattggga gggcattcgt ggtaaacgcc gcggcgaagg catgcgtgct cgaacgaaat
    32881 ttgcggcgca ggtcattctg gcggttggga ccgccatcgt gttgaaatac gttttggaag
    32941 tggaagaaat gtaccttccg ggcgtcgaag gtgagattga gttgggctgg tggtatatcc
    33001 caatcgcagc gttcgtgatc gtgagtgaat ccaacgccgt taattttaca gatgggctgg
    33061 atggtctttc cggtttaatt accgcaaccg catttgctgc atttggtggt atcgcattga
    33121 tcatcgggta tacctacctg gcccagtttt gcttcattat tgtcggagcg ttatttggct
    33181 tcctctggtt caatgtccac cccgccgaat tgatcatggg tgataccggt tcgcttgcac
    33241 tggggtccac gctggctgtg gtcgcactga tgacaggtcg ttggcccttg ttgatcatca
    33301 tcgccatcat tcccttgagc gaagccttga gtgttgttat ccaggtcctt tatttcaaac
    33361 tgacgaaggg caaacgtttc ttcaaaatgg ctcccattca tctgcatttt gaactgctcg
    33421 gctggagcga aacgcaggtt gttcaacgct tctggttgat cgggctgctc gcagccatgg
    33481 tcgggatggg actggccttt gtgtaaatga aaacaaattg gaacggcaaa cgcgtattga
    33541 ttttaggagc cgccaggcag ggactcgccc tggcgcgctg gctctccttg catggtgcgc
    33601 atgtgactct gaacgatgcg cacagcgaag atgaattacg tgtggcgcgt gagtcgttgg
    33661 ctgagtacaa gatagattgg gcatttggcg ggcatccgct tgaattgttg gattcaacgg
    33721 atgtgctctg tctctcaggc ggagttccac ttacactgcc gattgtggct gaagccgtca
    33781 agcgagggat tcctctctcc aacgattctc agatcttcat ggaagtagca ccctgtaaga
    33841 cagttggcat tacaggctcg gcaggcaaaa ctaccacgac gacccttgta ggacgaatgt
    33901 ctagaaatgt ctacggcgat cgggcttata ttggaggaaa catcggcgat cctctgatta
    33961 actacgtgga caacatgaat gccgatgata ttgctatcct tgaactttca tcgtttcaac
    34021 tggatcagat gacgatttcc ccaaatgtgg ctgccattct caatatcacc cccaatcatc
    34081 ttgatcggca tggcacgatg gaagcgtata ccgatgcgaa ggcgcgtatc cttgagtttc
    34141 agtcggagca ggacgttgct attctgagcc gcgacgatcg gggctcatgg aatttgaatg
    34201 ataaagtgcg cggcaattta tatgcgttta gttcgcaagc actggataaa gcgttgaatg
    34261 gagcctatct gcaggacgga tggctcaacc tgcgcgatgg aaacttgctc ctgcctttga
    34321 tctcccgcgg gcatatttca ttacgcggtg accataacgt ttcgaatgta ctggccgcgt
    34381 ttgcaattgg tcatgccgcc ggcttcgctc ccgaccccat gatgcaggcg gtgaaagact
    34441 tccatggtgt gcctcatcgc ttggagtttg ttcgggaact gcgcggtgtc tgctggtaca
    34501 acgattcgat tgcgaccgcg cccgagcgga gcatggcggc tatccgtgca tt
//
LOCUS       DASTJP010000893         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_984674, whole genome shotgun sequence.
ACCESSION   DASTJP010000893 DASTJP010000000
VERSION     DASTJP010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984674"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..882)
                     /locus_tag="VFQ23_26090"
     CDS             complement(64..882)
                     /locus_tag="VFQ23_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET9910142.1"
                     /translation="MPKVRLDVLLVERGLAESRAKAQAMIMAGQVRVAGQVALKPATA
                     IPSDSELTVDSGPRFVSRGGEKLDAALEAFGINVAGLICADVGASTGGFTDCLLQRGA
                     IKVYAIDVGKGILHWKLRNDPRVVVMEETNARFVESLPEPVSLVTVDASFISLKILLP
                     VVKVWTPLHLPPFSEKIGGEQKGSVIALIKPQFEAGKKDVARGDGVIRDPEIHKQVLL
                     DVLSFAQQTGFRVRGLIKSPLLGPKGNVEFLAWLSQAQDVEAREELGPIIETVL"
     gene            951..1820
                     /locus_tag="VFQ23_26095"
     CDS             951..1820
                     /locus_tag="VFQ23_26095"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HET9910143.1"
                     /translation="MLSALIKAMRLRQWTKNGFIFFALIFDKQLFSLEPFIRTLEGFF
                     LFCLISSAVYLLNDIADIEGDRQHPQKKHRPLASGLLPIGVALGAAILLAIVALGLGY
                     VLEPAFAGILAVYLTLNLLYSRWLKHIPILDVLIISSGFVLRVAAGVALITVERFSPW
                     LYMITILFSLYIGLGKRRAEMNLLEQGASAYRKVLDGYSIPLLDQYITIVSGMTIIAY
                     SLYTFSAPNLPVNHSMMLTIPFVVYGIFRYLQLIQTGHAAGAPDEVALKDRPLQITVL
                     LWGLAVIAVFYLS"
     gene            1847..2797
                     /gene="mvk"
                     /locus_tag="VFQ23_26100"
     CDS             1847..2797
                     /gene="mvk"
                     /locus_tag="VFQ23_26100"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET9910144.1"
                     /translation="MATASAPGKIILFGEHAVVYGRPALAIPVTQVHAEVEVLASDQA
                     GIWINAPDIDLHAELNTVPSDHPIASVIHNFLFLSRTSPALVGSGGKFPNLKINISST
                     IPVASGLGSGAAVTVALVRALAAHLNHPMADEKINGFAYEIEKIYHGTPSGIDNTVVT
                     YAQPVYFTKGKPIEIFKVGKPFTIVIGDTGISAPTKESVADVRKLWTENPKRFEVIFD
                     KVADISFAARRVIEEGWVDMLGTLMNENHAYLQEMTVSSPELDRLVSAARTAGASGAK
                     LSGGGRGGNMIALVEDHKAQNVAEALMGAGAKRTIITKLS"
BASE COUNT          666 a          763 c          660 g          720 t
ORIGIN      
        1 tatcggtaag tggaggcatg aaggaatttt accagacggg ctcaccagat aaatttggct
       61 tggttacaac accgtttcga tgattgggcc taattcttcg cgcgcttcaa cgtcctgtgc
      121 ctgactcaac caggccagga actctacatt tcccttcggc cccagcagcg gacttttaat
      181 caacccccgc acgcggaaac cggtctgttg agcaaaactc aaaacatcca gcaacacctg
      241 cttatgaatc tcaggatcgc gtatcacacc atcgccacgc gccacatctt ttttcccagc
      301 ttcaaattga ggtttgatga gggcaattac actccccttt tgctctcccc ctattttctc
      361 tgaaaatggg gggagatgga ggggggtcca cacctttacc accggcaaca atatcttcaa
      421 cgaaataaac gaggcatcca cagtcacaag cgaaaccggc tcaggcaaag actccacaaa
      481 ccgcgcgttg gtctcttcca tcactaccac gcgcggatcg ttccgtaact tccaatgcag
      541 aattcccttg cccacatcga tcgcgtaaac tttgatggca ccacgctgca gaaggcaatc
      601 cgtaaaacct ccggtggacg cgccgacatc tgcacagatc agccctgcga catttatccc
      661 aaaggcttcg agggcagcat ccagcttctc tccaccgcgt gagacaaagc gcggaccaga
      721 atcaacggtc agctcggaat cggaggggat cgcggtagcc ggtttgagcg cgacctgacc
      781 tgctacgcgt acctgccccg ccatgatcat tgcctgagct ttggcacgtg actccgccag
      841 tccacgttca acaagtagta catccaaacg aactttgggc atgcttgaat tgtaaccaat
      901 tcttcaaaca gcctgtacca attacgcttt ccttctgtta tcattaaccc atgctttccg
      961 cccttatcaa agccatgcgt ctacgccaat ggacaaagaa cggcttcatc ttttttgcgt
     1021 tgatcttcga caaacaattg ttttcgctcg agccgtttat tcgcacactc gaggggttct
     1081 ttttattctg tctgatctca agcgctgtct atctgctcaa cgatatcgct gatatcgaag
     1141 gtgaccggca acatcctcag aagaaacata ggccgcttgc atcaggattg ctgccgatcg
     1201 gcgttgcact gggggccgcg attcttttgg caattgtcgc attgggactc ggatatgttt
     1261 tggaacctgc ttttgcaggc atcctggcag tttatcttac actcaacctg ctttattcac
     1321 gctggcttaa acatattcct attctcgacg tgctgatcat ttcgtccggc ttcgttcttc
     1381 gtgtggctgc cggcgtagcg ctcattacag tggaacgatt ctctccctgg ttgtatatga
     1441 tcacaatttt gttctctctg tatatcgggc tgggcaaacg gcgtgcggag atgaatttgc
     1501 ttgaacaggg agccagcgcg tatcgtaaag tgctggatgg atactcgatc ccgctgcttg
     1561 atcaatacat tacgatcgtt tcagggatga ccatcattgc ttattcgctg tatacattct
     1621 ctgcgccaaa tctgcctgtc aatcacagca tgatgctcac aattcctttc gtggtctacg
     1681 gcatctttcg atacctgcaa ttgattcaaa ccggtcacgc agctggcgca ccagatgaag
     1741 tggcactcaa ggaccggccg ctccagatca ccgtcttatt gtggggactg gcggttattg
     1801 ctgtttttta tctttcgtaa ggcgcctggc aatttgccat ccctttatgg ccactgcctc
     1861 tgcccctggc aagatcattc tttttggcga acatgcagtc gtttatggaa gaccagcatt
     1921 agcgatcccc gtcactcagg ttcatgcaga ggtcgaggtt ttggcttccg accaagccgg
     1981 aatttggatt aacgctcctg atattgacct acacgcagaa ctgaacacag ttccttccga
     2041 ccatccgatt gcatccgtta ttcataactt tttattcctc tcgcgcacat cccccgcgct
     2101 ggttgggagc ggcgggaaat tcccgaatct aaagataaat atttcttcca cgatccccgt
     2161 cgcttccggg ctcggttcgg gcgcagcagt gacggtggca cttgtgcgcg ctttagccgc
     2221 ccatctcaat catccgatgg ctgatgagaa gatcaatggc ttcgcctatg aaattgaaaa
     2281 aatttatcac ggcacaccct ccgggatcga taatacagtt gtcacgtatg cccaaccggt
     2341 ttatttcaca aaaggaaaac ccattgaaat attcaaagtg gggaagccgt tcaccattgt
     2401 catcggcgat acaggaattt ctgcaccgac gaaggaatcg gtagctgatg ttcgaaaatt
     2461 gtggacagag aacccaaaac gctttgaggt cattttcgat aaagtagcag atatttcctt
     2521 cgctgcccgt cgcgtgatcg aagaaggctg ggtggatatg cttggcacgc taatgaatga
     2581 aaaccatgcg tatttacagg aaatgaccgt ttcttcaccc gagctggata ggctcgtctc
     2641 agctgcgcga acggccggtg catccggggc aaaactcagc ggagggggtc gcggcggcaa
     2701 tatgattgcc ctggttgaag accacaaggc acagaatgta gcagaagccc tgatgggcgc
     2761 aggagcaaaa cgaacgatca ttactaagct atcatgaaat gggtcgcgt
//
LOCUS       DASTJP010000894         4418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1461950, whole genome shotgun sequence.
ACCESSION   DASTJP010000894 DASTJP010000000
VERSION     DASTJP010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461950"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFQ23_26105"
     CDS             <1..288
                     /locus_tag="VFQ23_26105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910145.1"
                     /translation="QIVVFFALSVISGDARELGKFTVSEAEVFAIAPDEKRFAVSDAR
                     GVGRIVDIETGEVLSGFKSVEQTVDLAWHPDGATLAILDYKTKLQLWQIPR"
     gene            379..1530
                     /locus_tag="VFQ23_26110"
     CDS             379..1530
                     /locus_tag="VFQ23_26110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9910146.1"
                     /translation="MKQRVCLFIFIFLSACTSAPAIVSPTELLPSPQLVIQTLTPSPV
                     PTITLTPSPTLTTEEWISTFTIDGLRNHEFEGGEIKIRSTLDENDKYTTYLIEYPSDG
                     LTITGVMQIPAGDGPFPVIILNHGFFARSVFNSGDGTDRAAAYLAQQGYITLASDYRS
                     WGGSEIGESFFYSGLAIDVINLLNAVPSIEEADPNRVGMWGHSMGGGVTMKVLTIVGT
                     GSSRPELGKEASIKAAVLYSTVSADMSDIINRWGPGCFGDIAQGELIVGCNSSDVIPN
                     DLPLNLQDAYRFAASDADTLKEISPYYHLDVVSAPVQIHYGTEDGQLLSGTPPQWSVK
                     LTQGLRDAGKEAEMYQYEGEGHSFIGQPWFDFMNRTVRFFDENVKNNSR"
     gene            1626..1949
                     /locus_tag="VFQ23_26115"
     CDS             1626..1949
                     /locus_tag="VFQ23_26115"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET9910147.1"
                     /translation="MPYVVYILECADGSYYTGSAADLSKRLCEHETGASPSAYTFSRR
                     PVKLVWTSDECRKYSEALRWERQIKGWSRAKKQALIRGDFDAIHVIVKNERKKREQLK
                     KKPPR"
     gene            2091..3533
                     /locus_tag="VFQ23_26120"
     CDS             2091..3533
                     /locus_tag="VFQ23_26120"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HET9910148.1"
                     /translation="MNNVLPHLKMLTEEQIRDIHQYTLKLLATTGVRVDSPSAVEMLR
                     KKVGDSNVDGRTVRIPAELVEWAIKSAPRQIQVYDRRGNPQFTIGAEGDRTRFGIGVT
                     ALFYQEPETDTPVEFQRKHMQDMVRVGNKLPLYDMVSTIGIVRDVPDYLTDLIGSLEH
                     FANGIKPMVLLCSDEHKFDDVLRMFETLHGGDLGEKPFIIPYFNSVSPLVMNEGTVDK
                     MKIAIERGLPVVVSNYSLSGATTPITPAGTIAVLLAELLAGLVIGQLYKEGAPMLLGM
                     LPVYLDMKTLLNFYDPQSILVSLACAEMMAHYGIPHCGTSGSGTGWGMDLLAAETYWM
                     NTLMMTLTKGGIAPFVGDTLGSKAVSPLTFIYCHEIIDQALLFSGGFQLDDANVGLDE
                     IHAVGPGKGYVSAPTTLKKYKTGYYVSRIFPRWSMEKWLQAGQPHVQKHLKEYGIEFL
                     KDLPAPEDHEELIRKGEEFIGKWVVESGRQ"
     gene            3586..3924
                     /locus_tag="VFQ23_26125"
     CDS             3586..3924
                     /locus_tag="VFQ23_26125"
                     /inference="COORDINATES: protein motif:HMM:NF039191.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HET9910149.1"
                     /translation="MIYILREKPTSSQVNEMLLEYETMIKIVVDVRRRILAGGGDMHS
                     DCESALLDDGSEQDDLWGANWFPAGKEIVFESLINIRPRLGNRSILIQSEELRQQVES
                     VTRAIFGDTE"
     gene            3921..4274
                     /locus_tag="VFQ23_26130"
     CDS             3921..4274
                     /locus_tag="VFQ23_26130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910150.1"
                     /translation="MMSNKEKLRERFLRDTLPRRLGGLASTLGRISSVARKSSDPGNV
                     TRLLEEAKYLIEWTAADAEPEVAAELVSMQRLLNLWLKTWDKTNQTKEQRTLLSVQAK
                     LWSDRAMDFSGLLNV"
BASE COUNT         1152 a          949 c         1179 g         1138 t
ORIGIN      
        1 cagatcgtcg ttttctttgc gctgagcgtt attagtggcg atgcgcggga acttgggaaa
       61 tttacggtat cagaagcgga agtattcgcc atcgcgccag atgaaaaacg cttcgctgtt
      121 tcagatgcac gaggcgtagg acgaatcgtg gatatagaga ctggtgaggt gctgagcggc
      181 ttcaaatctg ttgagcagac cgttgatctg gcttggcatc cggatggtgc gactctagca
      241 atcctcgatt acaaaacaaa gctccagttg tggcaaatcc cgcgttagcg taatcctcta
      301 caattctgcg accaagttca tagatattca gcagagaccc ggcgttactg ggtctctgct
      361 gaatgataca attaattcat gaaacagcgc gtgtgcctgt ttatttttat tttcttaagt
      421 gcctgtacat ccgcacctgc gattgtttct ccaacagaac tgttgccatc ccctcagcta
      481 gttatccaaa cactgacgcc atcgcctgtt cccacaatta cactcacacc atccccaact
      541 cttacaaccg aagaatggat ttccaccttt accattgatg ggctccgcaa tcatgagttt
      601 gaaggcggcg aaatcaaaat ccgctctaca ctcgatgaaa atgataaata tacaacttat
      661 cttatcgaat atcccagcga tggattaacg atcacaggcg tgatgcaaat tccagcaggg
      721 gatggaccat tccctgtaat cattctgaat cacgggtttt tcgctcgcag tgtatttaac
      781 tctggcgatg gaacagatcg cgccgcggct tatcttgctc agcagggata catcaccctg
      841 gcgtccgatt atcgcagttg gggtggttcc gagattggcg aaagtttttt ttactctggc
      901 ttggccatag atgtaatcaa tctgttgaat gccgttccat ccatcgaaga ggcagatccg
      961 aatcgcgttg ggatgtgggg tcacagcatg ggtggtggtg taacgatgaa ggttttaacg
     1021 atcgtaggga cggggtcttc ccgtcctgaa ttggggaaag aggcctcgat caaagcggca
     1081 gtcttatatt cgaccgtcag cgcagacatg agcgatatta tcaatcggtg gggtccaggt
     1141 tgttttggag atatcgcaca aggcgaattg atcgtcggtt gtaattcctc tgatgtaatt
     1201 cccaatgacc ttccattgaa cttgcaggat gcctatcgtt tcgctgcatc ggatgcggac
     1261 acgctcaagg agatttcgcc atattatcat ctggatgtcg tgagcgcacc ggtccaaatc
     1321 cattatggaa cggaagatgg acagttactc agcgggacac cgccgcagtg gtctgtcaag
     1381 cttacacaag gattgcgcga tgctggcaaa gaagccgaga tgtatcaata tgaaggcgaa
     1441 gggcattcgt ttattggcca gccctggttc gattttatga accgcactgt tcggtttttt
     1501 gatgaaaatg tgaaaaataa ttcgcgctga ctatagttcc ctgttgggaa gcggagccgt
     1561 aggcgaagtc gaagcgccaa tgatcatcta gcccctgctc agaacgaaag agtaaataaa
     1621 aatttatgcc ctacgttgtt tatattcttg aatgtgcaga tggatcatat tacactggaa
     1681 gcgctgcaga tctaagtaag cgtctctgcg aacatgaaac tggtgcttca ccatcagctt
     1741 atacattttc aagacgcccg gttaaacttg tttggacttc agacgaatgc cgtaaatatt
     1801 ctgaagcact tcgttgggag cgacaaatca aaggttggag tcgagcaaag aagcaggcat
     1861 tgatccgtgg agactttgat gcaattcatg ttattgtgaa gaatgaaaga aaaaagagag
     1921 aacaacttaa aaagaaacct ccgcgctgag cggagggaca gcgattttct gtcccgtagt
     1981 cgaagcgcca atttcaagac aagttaatat attactagct gcacttcgac tatgttcgac
     2041 tgaagtgcac agtctcactc cgctcagcgc gagtgatgtt aggagaatta atgaacaatg
     2101 tccttccgca cctgaagatg cttactgaag aacagatacg ggatattcat caatacacgt
     2161 tgaagcttct ggcgacgact ggcgtgcgcg tggattcgcc gtcagcggtg gagatgctca
     2221 ggaaaaaagt gggcgattca aacgtcgatg gcagaactgt gcggattccc gcagagctag
     2281 tggaatgggc gatcaagtcc gccccgcgtc aaattcaggt ctatgaccgg cgcgggaatc
     2341 cgcagttcac gatcggtgca gaaggggatc ggactcgctt cgggatcggt gttactgcgt
     2401 tgttttatca ggagcctgag actgatacgc ctgtcgaatt tcaacgcaaa catatgcagg
     2461 acatggtgcg ggtgggaaat aaactgccgc tctatgatat ggtctcgacg attggtatcg
     2521 tgcgcgatgt ccccgattat ctgacggatt tgattggaag cctggaacac tttgcgaacg
     2581 gcattaagcc gatggtccta ttgtgttctg acgagcataa gtttgatgat gttctgcgca
     2641 tgttcgagac acttcacggt ggtgaccttg gtgagaagcc gttcattatt ccgtatttca
     2701 actcggtgtc gccattggtg atgaacgaag gcactgtgga taaaatgaaa atcgccatcg
     2761 agcgtggatt gccagtcgtt gtgtcaaatt acagtctgtc gggcgcgacc acgccgatca
     2821 cacccgcggg aacaattgcc gtgctgcttg ctgaattact tgcgggtttg gtaatcggac
     2881 agttgtataa agagggcgca ccgatgctgc tcggcatgct gccggtttac ctcgatatga
     2941 agaccttatt gaatttttat gacccgcaaa gtattctggt gagtctggca tgtgcagaga
     3001 tgatggcgca ctatggaatt cctcattgcg gcacatctgg gagtggcacg ggctggggaa
     3061 tggacctact agctgcggaa acatactgga tgaacacatt gatgatgacc ctcaccaaag
     3121 gtggcatcgc gccatttgtg ggtgatacac tcgggtcgaa agccgtctcg cctctgacat
     3181 ttatttattg tcacgagatc attgaccagg cacttctgtt ttcaggcggg tttcagttgg
     3241 atgatgccaa tgttgggttg gatgaaatcc atgctgtcgg tcctggtaag ggatatgtct
     3301 ctgcgccgac cacactcaag aaatataaga caggatatta cgtgagccgc atcttcccgc
     3361 gctggagcat ggagaagtgg ctccaagctg gtcagccgca tgtgcagaaa catttgaagg
     3421 agtatgggat tgagttctta aaggatttgc ctgcaccgga ggatcatgag gagttgataa
     3481 ggaaagggga ggagtttatt ggaaagtggg tagtagaaag tggtaggcag tgattagcta
     3541 tcagcaacga gttatgagaa cgactcagaa cggtataatc gcaacatgat ttacatactt
     3601 cgagaaaagc cgacaagtag tcaagtgaat gagatgctgc tggaatacga aaccatgatc
     3661 aaaattgtgg tagatgttcg tcgccgcatt ctggctggag gtggagacat gcattccgat
     3721 tgtgaatctg ctttgcttga tgatggcagt gaacaggacg atctatgggg ggcgaattgg
     3781 tttccggctg gaaaagaaat tgtgtttgag tccctgatta atatacgtcc aagattaggg
     3841 aaccgcagta ttttgattca aagtgaagaa ctccgtcagc aggtcgaatc tgttaccaga
     3901 gcaatttttg gagatacaga atgatgtcaa acaaagagaa actacgggag cgctttttgc
     3961 gcgacactct gccacgcagg ttaggcggat tggcatctac gttgggaagg atttcctctg
     4021 ttgcgcggaa atcgtcagac ccaggaaatg ttactcgttt actggaggag gccaaatatc
     4081 taattgagtg gactgctgct gatgcagagc cagaggtcgc ggcggaatta gtatccatgc
     4141 aaaggctgct aaatctctgg ttaaagactt gggataaaac aaatcaaact aaagaacagc
     4201 gtaccttgct atctgtccag gccaagcttt ggtctgaccg cgcgatggat ttttcagggt
     4261 tgttgaatgt gtaagaggag taagttgaga atgtaattaa atccgacctg agtccatgaa
     4321 attctccgaa gcgccgacgt cttcgcgcac cactatggat ttcgatgcac ttagcctgag
     4381 ctaacaggat ttacccgcgc cgcaaaatca tgatgatc
//
LOCUS       DASTJP010000895         5472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1461968, whole genome shotgun sequence.
ACCESSION   DASTJP010000895 DASTJP010000000
VERSION     DASTJP010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461968"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFQ23_26135"
     CDS             complement(<1..425)
                     /locus_tag="VFQ23_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9910151.1"
                     /translation="MADISSVSTTTRQVIAEVERAVVGKRALLEMMMASVLAGGHILL
                     EDFPGLGKTLIARSFAKALGLEFKRIQFTPDLLPGDITGGYIFDRVKNKFELRKGPLF
                     ANIVLADEINRASPKTQSALLEAMQEGQVTIEGESLPLP"
     gene            complement(494..1114)
                     /locus_tag="VFQ23_26140"
     CDS             complement(494..1114)
                     /locus_tag="VFQ23_26140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910152.1"
                     /translation="MTMMARRWLVIIGVGIIAALLAFPLRGVVHQLIVVPLSYLLWLF
                     GLLYRAMPQQWWWVVVTLLVLFVLGKSLLIETKTTRKQVPVSQVDRGKVESLALSMQK
                     ADRGIYSKWLVANRLGKLAHQILQQRESGIERSLFAPLVGQGWEPTTELQQYLEKGLH
                     GSFTEFPNRRYGLSGRPQKTALDHDVEDAIEFLESQVEGKTSLPRR"
     gene            complement(1111..1998)
                     /locus_tag="VFQ23_26145"
     CDS             complement(1111..1998)
                     /locus_tag="VFQ23_26145"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HET9910153.1"
                     /translation="MRSFLENRRLILVFSILTLSALTVLAIGLGNVPFRDAQRFGSVI
                     ERGNLGIDTQSLLTSFAEIPIWQSVVMWLLLLIMIVLVSVLLSPELRKALIRSIIRVA
                     VTYWAFYIVATRYREALAQMMSDGQALSGSIPYTFGGTPPPAFVPPQTISLTSYLVSF
                     GIAVLLVLFAWKMFKVWQEHNAVNANLPLNKIAGIARSSLNDLTSGRNSTDVIMNCYF
                     RMSDVVENKRNLSRSKSMTPAEFATRLEQGGLPSDAVQRLTRLFERVRYGGHRSGTSD
                     VNEAVACLTTILNHCGEAV"
     gene            2049..2579
                     /locus_tag="VFQ23_26150"
     CDS             2049..2579
                     /locus_tag="VFQ23_26150"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET9910154.1"
                     /translation="MTESINDLCKAPIHPEAAEGLRLFNEGKYFEAHEALEIAWLEEK
                     GKVRELYRGILQIAVVYLHATRHNYNGVIKVYERSQKWLKDWPDICRGIDVGQLQRDA
                     KAVLEEARRLGIDRFSQFDNSLLRPVQWVEHKAGKSHVYICDRCGHEMHEKNCKVICP
                     NCGNRFDCSDLNIYFD"
     gene            complement(2600..3295)
                     /locus_tag="VFQ23_26155"
     CDS             complement(2600..3295)
                     /locus_tag="VFQ23_26155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910155.1"
                     /translation="MQDRKYFGLTTPQLGILAGLLVIVCLLFGLTGWFVFRGGFARNS
                     APTAAILSTSTPFVIPTLTPTSIPTPVPYEELIPEGWVQYRTALVELWLPSQFENSGQ
                     GISGIAGNSVLLEMALTGQASGTSQYKIFVSVSFEPLSTGSLDQFLESRLSNIPAGIN
                     VSERRKVIINSTEAYRVLFESNNNNVLTNDLVFAILDGTTIWYVRYSAQINEFYEMLS
                     AFEQSIKTFRIVK"
     gene            complement(3460..>5472)
                     /locus_tag="VFQ23_26160"
     CDS             complement(3460..>5472)
                     /locus_tag="VFQ23_26160"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9910156.1"
                     /translation="AIAWWLNPLSLGALGYIFSNIRSWRFWTILALLVWGFVNFLVSS
                     SIVSETARILTFNGLLITFYILLGLLFLYIQLRVFIWAAAGKPARFFFGTMAWAAFNG
                     LIYWAIISPEVLNVVLVVLGLAFRVVFILFMAIIQFVAIFWFMARSRVETIRPGDPKS
                     LTFDDYKGQPNLLKMVRQWISLLSDRSQFQKMGGQFINGLLLYGEPGTGKTLLAKCMA
                     GEAGIAFISIEGSGFRAMFWGVDVLKMIAFINRARKLAKEYGACIAYIDEIDAVGMSR
                     GNVMGGQAGMMGGMGGMMNGTGALTRLLYEMDGIGELSRWEKLRARWYQLRKKPVPPR
                     DWHILFMGSTNRPDVLDPALTRPGRFDRTVVVNKPDRGGRREMVKYYLNKIRTDETVD
                     IEAIVSDTAWATPARIMSAITKDSVRLALFDDRDRVSQHDIELAFQEQAMGLENPIEE
                     MQEDQRRQVAYHEAGHAVVQYHLRPDERIVRVSIVRRSDALGYVLPVSNYDIYSLPLR
                     TFVADILVSMAGHVATKLFLGEYWTGATGDFQNVRGRIWQLAHYGYFGPPLDLQQGAN
                     IGKDKTEVVEKFWRKLEEQTEQLLTKHSAEVHAVAQALLERNDLTGKQCIEVIRAAAL
                     TDQADDSELLLKALVEDTIVHNNGKAEEKPKAKTKRKANPKLAAKN"
BASE COUNT         1474 a         1434 c         1272 g         1292 t
ORIGIN      
        1 tctggcaacg gcagcgattc gccttcgatc gtcacttgcc cctcctgcat ggcttccaat
       61 aacgcagatt gtgttttcgg agatgcgcgg ttaatttcgt cggccagtac gatgtttgca
      121 aagagcggac ctttgcgaag ttcaaattta ttttttacac gatcaaaaat gtagccgccc
      181 gtgatatccc ccggcaacag gtcaggcgtg aattgaatgc gcttgaattc aagccccagc
      241 gctttggcaa aactgcgggc gatcagcgtc ttgcccagcc cggggaaatc ttccagcagg
      301 atgtgtccgc ctgccaacac cgaagccatc atcatttcaa gcagagcgcg cttgccaacg
      361 actgcacgct cgacttctgc aatcacctgc ctggttgttg ttgaaacact ggaaatatct
      421 gccaccttac tctccctaat ttcaccacga agccgtgaag acttgcagaa aaattaatat
      481 gatcatcctg gtcttagcgt ctcggcaacg aagttttgcc ttccacctgt gattcgagaa
      541 actcaatcgc atcttcaaca tcgtggtcga gagctgtttt ttgcggtcgg ccggaaagtc
      601 cataccttct gttcggaaac tcagtaaatg aaccgtgcaa tcccttttcc aggtattgct
      661 gcaactctgt cgtcggctcc cacccctgcc ccaccagtgg tgcgaagaga gatcgctcga
      721 ttccactttc gcgctgctgc aggatctgat gagcaagctt acccagacgg ttcgcaacca
      781 gccatttcga atagattcca cggtcagctt tttgcatgga gagcgcaagg ctttccactt
      841 tccctcgatc aacttgtgaa accggtacct gctttctcgt agttttggtt tcgatgagaa
      901 ggcttttgcc taaaacgaac aagacaagga gcgttaccac tacccaccac cattgctgtg
      961 gcatagcgcg gtaaagtaat ccgaagagcc agagtaaata agagagtggc acgacaatta
     1021 actggtgtac aactcctcgt aatggaaagg ccagcagcgc agcgatgatc cctacgccga
     1081 tgatgacaag ccagcggcga gccatcatcg tcatacggcc tctccgcagt gattcaggat
     1141 cgtcgtcagg caggccaccg cttcgttaac atccgaagtc cctgagcgat gccctccata
     1201 tcgcacccgt tcaaacaaac gcgtcagccg ctggactgca tcagatggca atccgccctg
     1261 ttcaagccgt gtcgcgaatt cagccggcgt catggatttg ctgcggctaa gatttcgttt
     1321 attctcgacc acatcactca tacggaagta acagttcata ataacgtctg tggaattccg
     1381 cccggaggta agatcattca gggatgaacg tgcaataccg gcgatcttgt ttaaaggcag
     1441 gtttgcattg accgcattgt gttcctgcca taccttgaac attttccatg caaagagaac
     1501 aagcaaaacc gcaataccaa aactgaccag ataggaggtc aacgaaatag tctggggtgg
     1561 aacgaatgct ggaggagggg tgccgccaaa tgtataggga atgctgccag aaagcgcttg
     1621 cccatcagac atcatttgtg ccaacgcttc acgataacgt gtcgcaacta tataaaatgc
     1681 ccaatacgta acagctacgc ggatgatact cctgatcaac gctttcctta attcaggcga
     1741 taacaacaca ctaactaaga cgatcatgat cagcaacaac agccacatga ccacggattg
     1801 ccaaatggga atctcagcaa aggaagtcag gaggctttgc gtatcaattc ccagattacc
     1861 ccgttcaatc accgatccaa accgttgtgc atctcgaaag gggacattgc ccaacccaat
     1921 ggccagcacc gttaacgccg acaaagtcag gatagagaag acaaggatca atcgtcgatt
     1981 ttcaagaaag gaacgcatgc ctagtatgga attttaccat tgcgaatata atatgaaata
     2041 ccctccctat gactgaatcg ataaatgact tgtgcaaggc tccaattcac cccgaggcgg
     2101 cggaaggact acgtttgttc aacgaaggca aatacttcga agcccatgaa gcgttggaaa
     2161 tcgcatggct cgaagaaaaa gggaaagtcc gtgaactgta tcggggtatc ttacaaattg
     2221 cagtagtcta cttgcatgcc acgcgccata attacaacgg cgtgataaag gtctacgagc
     2281 ggagccagaa gtggctaaag gactggccgg atatttgcag agggattgac gtcggtcagt
     2341 tgcaacgaga cgcaaaagcg gtgctcgaag aggcccgtcg gctggggatc gaccggtttt
     2401 cacaattcga caactcactg ctcagacctg ttcaatgggt tgaacacaaa gcggggaaaa
     2461 gccacgtcta catctgcgac cgctgcggtc acgaaatgca tgagaagaat tgcaaagtga
     2521 tctgcccgaa ctgcggcaat cgctttgatt gctcagactt gaacatttat tttgattagg
     2581 ctacagcgac gctgtgtctc tatttgacaa ttcgaaacgt ctttatgctt tgctcaaagg
     2641 cagacagcat ttcgtaaaat tcattaatct gggcggaata tcttacatac caaatggttg
     2701 tgccatccaa aatcgcaaat accaggtcat tcgtcagcac gttattgttg ttggattcaa
     2761 acaggacacg ataagcctcg gtcgaattga ttatgacttt tcgtcgttcc gatacattaa
     2821 tcccggcagg gatgttagat aatctggatt caagaaactg atccaatgaa ccggtactca
     2881 agggttcgaa ggaaacagaa acaaatatct tgtactgcga tgtaccggag gcttggcccg
     2941 tgagagccat ttccaataga actgaatttc ctgcaattcc agagataccc tgacccgaat
     3001 tctcaaactg ggatggcagc caaagctcaa ccagggcagt ccgatactgt acccatcctt
     3061 caggaatcaa ttcttcgtat ggaacgggtg ttggtatact cgtaggggtt agcgtcggaa
     3121 ttacaaatgg cgtggacgtg gaaaggatcg ctgctgttgg agcagagttt cgagcaaaac
     3181 cacctcggaa tacgaaccag ccggtcaagc caaaaagtag acaaacaatc accaacaacc
     3241 cagccaggat tccaagttgc ggcgttgtga gcccgaaata tttgcgatcc tgcatagcgc
     3301 ctccccaatc atgggtaatg aatgttcaaa tgtctaccag tatcttaacc ccttatccac
     3361 tttttcgacc gttttaagat catgattaac gaagttgtta atgagcgctc ttaacaaaaa
     3421 actggctgga gagcttccag ccagttttct ctttgtatgt tagtttttag cagccaactt
     3481 tggattcgcc ttgcgcttgg ttttcgcctt aggcttttct tctgcttttc cattattgtg
     3541 cacaatcgta tcttccacaa gggctttcaa caagagttca ctgtcatccg cctggtcggt
     3601 caaagcagcg gcacgaataa cctcaatgca ttgcttaccc gtcaaatcat ttcgttccaa
     3661 caatgcctgg gccactgcat gcacctccgc agagtgtttc gtcaggagtt gctccgtttg
     3721 ctcttcgagt tttcgccaga atttttcaac cacttcggtt ttatctttac cgatgttagc
     3781 accctgttgc aaatccaacg ggggaccgaa ataaccatag tgtgccagtt gccaaatacg
     3841 tcctcgaaca ttctggaaat ctcctgttgc accggtccaa tattcgccga ggaataattt
     3901 ggttgctaca tgtccagcca tcgagaccag gatatctgca acgaacgtgc gtaatgggag
     3961 ggaataaatg tcgtaattgg atacgggcag tacatatccg agtgcatcag aacggcggac
     4021 aatgctcaca cgtacaatgc gttcgtctgg gcgaagatga tattgaacca ccgcgtgacc
     4081 tgcttcatga taagccacct gcctgcgctg gtcttcctgc atttcctcaa tggggttttc
     4141 caagcccatc gcctgttcct ggaaagccag ttcgatgtcg tgctgtgaca ctcgatctcg
     4201 atcatcaaac aatgcaagac gcactgaatc cttcgtgatg gcagacataa ttcttgccgg
     4261 cgtcgcccag gcggtgtcac tgacaatcgc ctcaatgtca accgtctcat cagttctaat
     4321 tttgttgaga tagtatttca ccatctcgcg acgaccgcca cggtccggtt tgttcacaac
     4381 aacggtgcgg tcaaaacgcc cagggcgggt caatgccggg tcgagcacgt ccggccggtt
     4441 ggtggatccc atgaaaagga tatgccaatc gcgcggcggg actggtttct tgcgaagctg
     4501 ataccagcgc gcacgcagtt tttcccagcg gctcagttca ccgataccat ccatttcata
     4561 aagcagacgg gtcagagcgc ccgtgccatt catcataccg cccataccac ccatcatccc
     4621 ggcttgtccg cccatcacat tgccacgcga catacccacc gcatcaattt catcaatata
     4681 tgcaatgcat gctccatact ctttcgccag tttgcgagcc cgattgatga atgcgatcat
     4741 tttgagtaca tccacacccc agaacatcgc gcggaagccg gaaccttcaa tcgagatgaa
     4801 tgcgatacct gcctcaccag ccatacactt cgcgagcagc gtctttccag ttccaggttc
     4861 gccataaagc agcaagccat tgatgaactg accacccatt ttctggaatt gactccggtc
     4921 tgaaagaagt gaaatccatt gccgtaccat cttcaacagg tttggctgcc ctttgtaatc
     4981 gtcgaaggtt aatgatttgg ggtcaccagg gcgaatggtc tccacgcgcg aacgcgccat
     5041 gaaccagaag atcgccacaa actgaatgat cgccatgaag aggataaaga cgacgcggaa
     5101 tgcaagaccc agcacaacaa ggacgacgtt caatacttca gggctaatga tcgcccagta
     5161 aatcagtccg ttgaacgcgg cccaggccat cgtcccgaag aagaaccttg ctggcttccc
     5221 tgccgctgcc caaataaata cgcgtaattg aatataaagg aataataaac ctagcaggat
     5281 ataaaacgtg ataagcaaac cattgaatgt caggatgcgc gcggtctcag aaacaatcga
     5341 agaggagacc aaaaaattca caaatcccca gacaagcagc gcgaggatcg tccaaaaacg
     5401 ccaggaacga atgttgctaa aaatatagcc aagcgcaccc agggaaagcg ggtttaacca
     5461 ccaggcgatc gc
//
LOCUS       DASTJP010000896         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1250075, whole genome shotgun sequence.
ACCESSION   DASTJP010000896 DASTJP010000000
VERSION     DASTJP010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250075"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2255)
                     /locus_tag="VFQ23_26165"
     CDS             complement(<1..2255)
                     /locus_tag="VFQ23_26165"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9910157.1"
                     /translation="MAKAQTPKDHEDNVMKDQVRNKFEKMFSDRDLPTHASAREVDAM
                     KARIVELETALAKRSETQQANGKATYERQVAAHDSRNQDRFSNWSSAKEIPSDSSNRI
                     QNAKRTLSLLLGAIILSLPIYFYWAVKTGAWQIYAISIGLIFSGILIGFAISLVRRHR
                     VETAMEMMIANACLIIPLIVGLISAVGVVLAATQFLIILAITGQSLSGSRATRALLVG
                     FLFSIVTLIMDFTAPWSRLSVPGLQSTIPVIALTLVVALGFLLVRQFQNYSLRNKLIV
                     TFVGVALVSISAVGYVTNRVATAQLNEELGTNFNELASHMARETSDTIISNKIALDGL
                     VLNKFIQDSVEQANQTGTHNVSFMKYLDHQWGVSNIDDELVEQVVANPVAGELRELQD
                     RLPQYAELFVTNQYGAVIAATDQTSDYYQADEEWWQNAWNDGKGNIFVSQPEFDESAG
                     IYAIDIALPIPAHNRSNIIGVLRATVNITELTTLLTAGQFNQTGQAILVFPNHQFLTK
                     ETGVELNTLDAESILAISSIDGAFAKFEYNSIPSLVSKAPVVSLRDGRDNEVIEKLGW
                     VVVVHQDLDEANQPIAATTRGIMLAAIAVLIAAGMLALFVGGLFSKPIENLTAVAEQF
                     AAGDLLAKADDRSKDEIGKLATTFNNMTVQLRETLLGLEQRVAERTYDLELASEVGRS
                     VSEKVGDLNEMLSRSVELIRSKYELYYTQIYLIDAAGRNLVMRAGTGSTGTQLLQRGH
                     RLMISSTSLNAR"
     gene            complement(2292..>2772)
                     /locus_tag="VFQ23_26170"
     CDS             complement(2292..>2772)
                     /locus_tag="VFQ23_26170"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9910158.1"
                     /translation="GHKISIGKGLVGLSAKNNTSILVPDTSSSPNWLPNPLLPETRAE
                     VAVPISIGNQVLGVLDVQHNITNGLSQDDADLLFSIAHQVAFAIRNARSYSEVARRAE
                     RETLIAAISQKVQNTTTIESALQVAARELGSVLGTRDTRVVLKAGALQSNGKSKLAG"
BASE COUNT          699 a          674 c          636 g          763 t
ORIGIN      
        1 cgtgcgttca atgacgttga tgagatcatg agtcggtgcc cacgctgcag caattgtgta
       61 cctgtgcttc ctgtacctgc ccgcataacc agattccgtc cagcagcatc tatgagatag
      121 atctgtgtgt aataaagttc atacttcgag cgaatcaatt ctacggaccg tgaaagcatt
      181 tcgttcagat cgcctacttt ttcagatacc gaccgcccta cttcagaggc caattccagg
      241 tcgtatgtac gttcagcgac gcgttgttcc agcccaagca aggtttcgcg caattggacg
      301 gtcatattgt tgaaagttgt tgccagcttg ccaatttcat cttttgatcg atcatccgct
      361 tttgcaagca aatcccctgc tgcaaattgt tctgcaaccg ctgtcaggtt ttcgattggt
      421 tttgagaata gaccgcccac aaaaagtgcc aacatgccag ctgcgattaa cactgctatc
      481 gcggcaagca tgattcctcg agtggtagca gcgatgggtt ggttggcttc gtctaaatcc
      541 tgatgaacta caaccaccca gccaagtttt tcgattacct cgttgtcccg tccgtctctt
      601 aatgaaacta caggtgcttt gctcaccagg ctcggtatac tgttgtattc gaactttgca
      661 aatgcaccat cgatggatga aatcgctaaa attgactctg catccaaagt gttcagttcg
      721 acaccggttt ctttggtgag gaattgatga tttgggaata ctaagattgc ttgacccgtt
      781 tggttgaatt gacccgctgt gagcagagtg gtcagctcgg tgatattaac agttgctcgc
      841 aaaacaccaa tgatgttaga acgattatgt gccggtatcg gcaacgcgat atcaattgca
      901 taaatacctg cactttcatc gaattccggc tggctaacaa aaatattacc tttgccatca
      961 ttccaggcat tctgccacca ttcctcgtcg gcctgataat aatcactggt ttgatctgtg
     1021 gcggcaataa ctgcgccgta ttgatttgtt acaaaaagct cagcgtattg cggtaaacga
     1081 tcctgcagtt cacgaagctc accggccact ggatttgcca ctacctgttc aacgagttca
     1141 tcatcgatgt ttgacacgcc ccattgatgg tcgaggtatt tcatgaaaga aacattgtgc
     1201 gttccggttt gattcgcctg ctccacacta tcctgaataa atttgttgag aacaagtcca
     1261 tcgagagcaa tcttattgct aataattgtg tcgcttgttt cacgcgccat atgagacgcc
     1321 agttcattaa agttcgttcc cagttcttca ttgagttggg ctgtagcaac tctgtttgtt
     1381 acatagccta ccgcactgat cgataccagc gcaaccccca caaaggtgac gataagtttg
     1441 ttacgtaacg aataattttg aaattggcgg accaatagga aacccaatgc cacgacaagc
     1501 gtaagcgcaa tcacaggaat tgtggattgc aatcccggca ctgacaaacg agaccatggc
     1561 gcagtaaagt ccatgatcag ggtaacaatg gagaataaga aaccaaccag aagcgcacga
     1621 gtagcgcgcg aaccggataa cgattgccca gtaattgcca ggatgatcag aaactgggtt
     1681 gccgcgagca caactcctac tgctgatatt agcccaacaa tcagaggaat aataaggcag
     1741 gcgttggcga tcatcatttc catggctgtc tcaacacgat ggcgccgaac tagcgaaata
     1801 gcgaacccaa tgagaatgcc agaaaagatc aggcctatac taatggcata aatctgccat
     1861 gcaccagttt ttaccgccca gtagaaataa atcggcagag aaagaataat ggctccgagt
     1921 agcagggata aagtgcgttt ggcattctgg atgcgattgc tgctatcaga tggtatttct
     1981 tttgctgaag accagttgga aaaacgatcc tggttccggg agtcgtgagc ggctacctgt
     2041 ctttcatagg ttgcttttcc gtttgcctgt tgagtttctg atcgctttgc gagtgcagtc
     2101 tctagctcaa cgatgcgcgc tttcatggca tcaacttcgc gcgcgctggc gtgcgtgggg
     2161 agatcccgat ctgaaaacat tttctcaaat ttattccgaa cctgatcctt catgacatta
     2221 tcctcatgat ctttaggtgt ttgagccttt gccataataa ttccttcctt cgtctctttt
     2281 atcgaagaga cctatccggc caatttactc ttcccgttgc tctggagggc gccggctttc
     2341 agtactacac gagtatctcg tgtaccgagg acacttccca actctctggc ggcaacctgt
     2401 aatgcgctct ctattgtggt tgtattctgg accttttgac tgattgcagc aatcaatgtc
     2461 tcacgttccg cgcggcgcgc cacctcggag taagaacggg catttcgtat cgcaaacgct
     2521 acctggtgcg cgattgagaa taatagatcc gcgtcatcct gacttaatcc gttagtgatg
     2581 ttgtgttgta catccaaaac acccagtact tgattcccaa ttgaaattgg taccgcaacc
     2641 tcagctctcg tttccggaag gagaggattg ggcaaccagt ttgggctgga agaagtgtca
     2701 ggtaccaata tggatgtgtt gtttttcgct gagagtccta ccagtccctt accaatagag
     2761 atcttatgac cg
//
LOCUS       DASTJP010000897         4960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_425498, whole genome shotgun sequence.
ACCESSION   DASTJP010000897 DASTJP010000000
VERSION     DASTJP010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425498"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            594..4427
                     /locus_tag="VFQ23_26175"
     CDS             594..4427
                     /locus_tag="VFQ23_26175"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9910159.1"
                     /translation="MTVEITFGQHVKIHRQQLGLTQKQLAYRVGCASVTLRKIESDDL
                     RPSGQIAERLAKALKLSSRKRAAFVRFGRGQQSPSDLAQLLNDETKIEGRAIDYEPTY
                     ITTLTAADNPYKGLRAFDESDATDFFGREALIQQLLSRIGTGGDLARFLTVVGPSGSG
                     KSSVVHAGVIPALKRGALPGSENWLITALVPGSNPLEELEAALLQISSKPHLRLIETL
                     RKDSFGLLRAVNDCLPDDPAVELLMVIDQFEEVFTLVQEEEMRTHLLTSLLAAVLDER
                     SRVRIVVTLRADFLDRLLNFMDFSELVSQRTEFILPLTTEELERAIESPAKRVGLKID
                     DGVVPAIIHDLGDQPGALPLLQYSLTELFEKRKDNTVTRVAYQAIGGVLGAVGKRAEQ
                     VFTGLDVASQGMARQLFLRLVTLGEGVEDTRRRVLLSELESLVKYGSNEGSLSATETK
                     PVLDSFGQARLLTFDRDPLTSEPTVEIAHEVLIQAWSRLRNWLADSRENLHVQRQIMS
                     ATREWISGKQDPSFLATGARLVRFESMATDSDLTLNDEERAFLNSSVAERERREEEIT
                     ARQRQELNRQRWIIMGLTVFLIVAIGLSLFAFNQRRAAQTQAEIAFSRQLAAQALAEV
                     QKPLGNDEYGALLAIRSLKYQYDPVADAALVDAAGKLPIREFKGHSDSIRSIAISPTG
                     KYLLTGSADMTLKLSEIATGKEIRTFYGHNGEILDVAFSPDGKSVLCGSMDTTAKLWD
                     VTTGQEIYTLQGELGEDLSVAFSPDGKYILTTSRGTPAQLWEAATGHKVRTFAQVDAN
                     RGAVFSPDGKYILAASDENTAKIWETNTGREVQILRGHVNGVFSTAFSADGKYALTGS
                     IDNTAKLWDVSTGQEVYTLRGHSGSVRSVAFSPDSKYILTGSADRTARLWDFATGQEV
                     RTWRGHSQRVLSAVFSPDGKFILTGSQDGIAKLWDFAIGQGRILRGHSGEIYGTAISS
                     DGTYILTSGMDSTARVWDISTGEEVGIFSGHEGVVYSVSISPDAKHMLTSGQDGMAQV
                     WDIATGQGVRMFSGHFGPVYDAVFSPDGTHILTAGDDRTAKLWETSSGRELRTFSGHK
                     AAVFGVAFSPDGSYLATGSDDGTTKIWNVDSGEVMQTFQGPEIVYEVTFSSDGKYLLT
                     GNGDSTAQLWDVAAGKEVLTLNGHTNTVYDVAFSPDDKYAVTASADRTAKIWNLATGE
                     ELRTLSGHSSAIWSAGFSPDGKFVLTGSLDGTARLWEVDYRDFVVNVCARLFRDFTEE
                     ERIQARIEDQDPACP"
     gene            4735..>4960
                     /locus_tag="VFQ23_26180"
     CDS             4735..>4960
                     /locus_tag="VFQ23_26180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910160.1"
                     /translation="MTSTNKPFNSCVAAVTILSLIILATVVSPPSAKAAAGDPVTLGF
                     NSLPSAQGWTYTDGGSGVPEASVFSVDGSRL"
BASE COUNT         1293 a         1107 c         1315 g         1245 t
ORIGIN      
        1 cgtcctccac gacaatgtca ttcgtagtgg cgcggccaat ggaggttttg ccattcccca
       61 tgtcgtacgt tttcggaaaa ctatctggaa tatgaacgat gagattccac atactcaacc
      121 atttgccact ccccgtgaag atgtgtgggt tgatgaagag ggcatatgaa gccttaggat
      181 tgtacgttca cctctgatta taacctctat cgagaaattg actttataac ggattgtcca
      241 acttcgagaa taaagcgttt tgtcaccctg agcagcgaag ggtctctacc gctgcgcggc
      301 ggcattcttc gctcctgtcc gtcgctcaca ccgtccggaa gcggtgtccc ttcgggagaa
      361 tcacattgtg tttctgatgt tggacactcc cctttataac atttatcatg gtttacgccc
      421 gataaggata atattcatgg ataaaaacat agtgaagagt tagagagaaa atcatgatat
      481 tgaattttta aaaacagaat gctatactac aagaaaattt aatgtgttaa cctagaaaat
      541 aagccgggga ttatatattt agttttttgt caaatccacg tgggaaggtc agcatgactg
      601 tggaaataac gtttggtcag cacgtcaaaa tacatcgaca gcaattaggg cttacacaga
      661 agcagctggc ctatcgtgtg ggttgtgcat cggtgactct gcgaaaaatt gaatctgatg
      721 atctccgtcc ttcaggtcag attgctgaac gattagcaaa agcgcttaaa ctttcttcga
      781 ggaagcgcgc tgcgtttgtt cgatttgggc gagggcaaca aagcccctca gatttggctc
      841 aacttttaaa cgatgaaaca aagattgaag ggcgggcgat tgattatgaa cctacataca
      901 taactacact aaccgcagct gacaaccctt acaaaggcct gcgcgctttt gatgagtctg
      961 atgcaacaga ctttttcgga cgcgaggcac tgatacagca acttctctct cgcatcggaa
     1021 cgggcgggga tttggcgcgt tttcttacgg tcgttggtcc cagtgggagc ggcaaatcgt
     1081 ctgtagtaca cgcaggtgtt atccctgctc ttaaacgcgg cgccttgcct ggttcagaga
     1141 attggttgat tacggcattg gtgcctggtt ctaatccatt agaagaactc gaagctgctc
     1201 tcttgcaaat ctcgagtaag ccgcatcttc gcctgattga aacgttgcga aaagacagtt
     1261 ttggcttgct gcgcgcagtg aacgattgcc ttccagacga tccggctgtc gaactcttga
     1321 tggtgatcga tcagtttgag gaagtcttca ccctcgttca agaggaagaa atgcgcaccc
     1381 acctgctcac ctcactcctg gcggcggtgc tggacgagcg tagccgtgtg cgcatcgtcg
     1441 ttaccttacg cgcagatttc cttgaccgtc tattgaattt tatggatttc agtgaactgg
     1501 tgagtcagcg tactgagttt attcttccgc tcacaaccga agaactggaa cgagcgattg
     1561 agagtcccgc aaaacgtgtt gggctaaaaa tagatgatgg cgttgttcct gcaatcattc
     1621 acgatctggg cgaccagccc ggggcattgc ctttacttca atattcgctc accgaactat
     1681 tcgaaaaacg caaagacaac acagtaacaa gagttgctta tcaagcgatt ggtggcgtgc
     1741 tgggcgcagt gggaaaacgc gccgagcagg tattcacagg gttggatgtc gcaagccagg
     1801 gtatggcacg ccaattgttc ttgcgccttg tcacattagg ggaaggtgtg gaggatacac
     1861 gtcgccgagt actcctgtcg gagttagagt ctttagtcaa gtacggaagc aatgagggtt
     1921 cgctcagtgc aacagaaact aagccggttt tagattcgtt tggccaggcg cgcctgctta
     1981 cctttgaccg tgatccgttg acgagtgaac caactgtcga aatcgcgcat gaagtgctca
     2041 ttcaagcttg gagccgttta cgaaattggc tggctgatag ccgcgagaat ttgcatgttc
     2101 aacgccaaat catgtcagca acccgagaat ggattagtgg aaaacaggat ccaagttttc
     2161 tagcgaccgg cgctcgattg gtcaggtttg aatccatggc tacagattcg gacctgacgc
     2221 tcaatgatga ggaacgcgca tttctgaatt cgagcgttgc agaacgtgaa cggcgggagg
     2281 aggaaatcac agcccggcag agacaagagc tgaatcgaca gcgttggatc atcatgggtt
     2341 tgactgtgtt tctaattgtg gcgattggat tatcactatt cgctttcaat cagagaagag
     2401 ctgcccagac tcaagcagaa attgcctttt cgcgccaact agcagcgcag gctctggccg
     2461 aagtacaaaa accacttggc aacgatgaat atggcgcatt attagccatc cgctcattga
     2521 agtaccaata tgatcctgta gccgatgcgg cactcgtgga tgcggcaggc aaactgccaa
     2581 taagagaatt taagggtcac agtgattcga tcagaagtat agcaatctcg ccaactggga
     2641 aatacttgct aactggcagt gcggatatga cgctcaaatt gtcggagatc gcaactggga
     2701 aggaaatccg caccttttat ggccataacg gtgagatttt ggatgtagca ttctcaccag
     2761 atggtaaatc tgtgctatgc ggtagtatgg acacaacggc aaagttatgg gatgttacca
     2821 ctggccaaga gatatacact ttgcaaggcg aattgggaga ggatttgagc gtagcgttct
     2881 caccggacgg caagtacata ttgactacca gccgaggcac gccggctcaa ttatgggagg
     2941 ccgcaactgg gcacaaagtg cgcacgtttg ctcaagttga tgcgaaccgc ggtgctgtct
     3001 tttcgccaga tggcaagtat attttagctg ccagcgacga gaacacggct aagatttggg
     3061 aaaccaatac cggacgcgaa gttcagatcc ttcgtggcca tgtcaatggg gttttcagca
     3121 ctgcattttc tgcagacggt aagtatgcat tgacgggcag catagacaat acagcaaaac
     3181 tttgggatgt atcgacggga caggaagtct acacgcttcg tggtcacagt ggctcagtcc
     3241 gaagtgtagc attttcgcca gatagcaaat atatattgac aggtagtgca gaccgcactg
     3301 ccagattgtg ggattttgct accggacagg aggtgcgtac ctggcgcggc cactcgcaaa
     3361 gggttttaag tgcagtattc tcaccagatg gcaaattcat tttgacgggc agccaggatg
     3421 gtatagccaa gctgtgggac ttcgcgatcg gccaaggacg cattttaagg ggacacagtg
     3481 gagaaattta cggcactgcc atctcctcag atggcacata catcttgacc agtggcatgg
     3541 atagcactgc aagagtctgg gacattagca caggcgaaga ggtcggcata ttttctggac
     3601 acgagggggt ggtttatagt gtttccattt ctcctgatgc caagcatatg ttgaccagtg
     3661 gacaggacgg gatggctcaa gtgtgggaca ttgccaccgg tcagggggta cgcatgtttt
     3721 ctggtcattt tggtcctgtt tatgatgcgg tgttctcacc ggatggtaca catattctga
     3781 cagcgggcga tgataggacg gccaaacttt gggaaacgtc cagcggaaga gaattgcgaa
     3841 catttagcgg tcacaaggcg gccgttttcg gcgtagcctt ctcgccagat ggaagctatc
     3901 tggcgacggg tagcgatgac ggtacgacta aaatctggaa cgtggatagc ggtgaggtta
     3961 tgcagacctt ccaaggacca gagatagtct atgaggtaac gttttcttct gatggtaaat
     4021 atcttttgac ggggaatggc gattcgaccg cgcaattgtg ggacgttgct gcaggtaaag
     4081 aagtcctcac actgaatgga cacacgaaca cagtttatga tgttgcgttt tctcctgatg
     4141 acaagtatgc ggtgacggcc agcgcggacc gcacggctaa gatttggaat cttgccacag
     4201 gtgaggaact gcgcacctta agcggacata gcagcgccat ctggagcgca ggcttttccc
     4261 ctgacggaaa atttgtcctg acgggcagtc ttgacggcac agccagactc tgggaagtgg
     4321 attatcgcga ttttgtggtg aatgtctgtg cgcggttgtt tcgtgacttc acggaggaag
     4381 agcgcatcca ggcccgtatt gaagaccaag atcctgcatg tccatagttg gaaaagagtt
     4441 tttacgattt agtggggttt atcattctca gccaagcgat ataaaactga tacaaaatcg
     4501 ataactgcat tgttttcact ctttaattct gctaaattgg ttttaccgat tcgatggctt
     4561 gtatagcaga tcagcaggct ttatatcccc gtaaatattg ctattaggga atgaatggca
     4621 gtagggcctg ttagaaataa agaggtaagc ccaaaaaaaa tacatcaaaa cagcctacag
     4681 atgatgccaa tggtaaatct tcaaaaaaca aatgatagca gaaaggatca agtcatgaca
     4741 tccacgaaca agccttttaa ctcatgtgtc gctgcagtaa caatactctc gttgatcata
     4801 ctcgctacag ttgttagccc accatcggcc aaagccgcgg caggtgaccc tgtcacacta
     4861 ggtttcaact cactacctag cgcacagggc tggacctaca cggatggagg cagcggtgtt
     4921 cctgaagcaa gtgttttttc tgtcgatggc agtaggctgc
//
LOCUS       DASTJP010000898         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_193865, whole genome shotgun sequence.
ACCESSION   DASTJP010000898 DASTJP010000000
VERSION     DASTJP010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193865"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFQ23_26185"
     CDS             <1..623
                     /locus_tag="VFQ23_26185"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET9910161.1"
                     /translation="PTDSFRLANHLLDLGLYRTSIFAAREVLTLAGLDSQSESLTGPS
                     YFNHVRYGLYYPDLIIPEAQNYGHDPLFIYSIVRQESLFEGFVRSTAGAHGLMQVIPD
                     TGAQLATELNWPTSYDSEDLYRPNVSVRFGSYYLRKNRDQLGNNLYAALAAYNAGPGN
                     AIVWRDLAGDDPDLLLEIIRFQETRDYIRFISEIFNTYRTFYSPVQ"
     gene            complement(701..1012)
                     /locus_tag="VFQ23_26190"
     CDS             complement(701..1012)
                     /locus_tag="VFQ23_26190"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET9910162.1"
                     /translation="MTDRKFNLHSGQKGSALAVRVTPRASRNEIVEMLDDGTIKVRIA
                     APPVDDEANTALIEFLADILGVPKSRLDIVAGESGRDKLISVLDMDVETAHQRIVAHM
                     G"
     gene            complement(1016..1279)
                     /locus_tag="VFQ23_26195"
     CDS             complement(1016..1279)
                     /locus_tag="VFQ23_26195"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET9910163.1"
                     /translation="MIDVLIIMVNTVQQILVILVIFATILSFFMDPYHPVRRGVDNIV
                     QPMLDPIRRIVPLVGMFDFSPLILILLLQLIGRLLVAFLFSLR"
     gene            complement(1276..2052)
                     /locus_tag="VFQ23_26200"
     CDS             complement(1276..2052)
                     /locus_tag="VFQ23_26200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET9910164.1"
                     /translation="MTDLVKSIRENYQATLDHIASAARISNRQPEDVRLVVVTKSQPM
                     EVAEAAIEAGVRILGENYPEEGVTKIQSLRGQSGVEWHMIGHVQSRKARLVVDHFALL
                     HSLDSLKLAQRLNRFATESNRTLPVLLEFNTGGEESKSGWNASEESKWNTLFPEIQSI
                     LELPNLSVRGLMTMPPLGSNAEASRPFFIHLRLLRDRLAMQFPQTDWRELSMGTSVDY
                     AVAVEEGATLVRVGTAIVGARTYQHSMSHNGSVDKSEREA"
     gene            complement(2137..2910)
                     /gene="pgeF"
                     /locus_tag="VFQ23_26205"
     CDS             complement(2137..2910)
                     /gene="pgeF"
                     /locus_tag="VFQ23_26205"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET9910165.1"
                     /translation="MPLIQHNGLRYFQFDTLQTRHAVFTRHGGVSPEPWSSLNVGGTV
                     GDDLERVRENRLLSFYALGRAPETAFEVWQVHSADVVCARAPRPHDESLRQADIILTD
                     TPEVTLFMRFADCVPILLHDSRQGVVGLAHAGWRGTLHDVATATVDAMRKQYDSNPAD
                     ITAGIGPSIGPDHYQIGADVILQVMQKYGDESETLLKSHNGKIHFDLWKANHLLLERA
                     GVGNIEHAEICTACHTEDWFSHRAEKGRTGRFGALISLQ"
BASE COUNT          750 a          780 c          703 g          743 t
ORIGIN      
        1 gtccgaccga cagcttccgc ctggcaaatc atctgcttga tcttggctta tatcgcacgt
       61 caatttttgc tgcacgcgag gtgctaacgc tcgccgggct ggatagtcaa tctgaatcgc
      121 tcacaggtcc ttcttacttc aatcacgtcc gctatggact ttattatcca gatttaatca
      181 ttccagaagc tcaaaactat ggacatgatc cgttattcat ttatagcatc gtccgtcagg
      241 agagcttgtt tgaaggattt gtgcgttcca ctgcgggtgc gcacggactc atgcaggtca
      301 ttcccgacac aggcgcacag ctggctactg aactcaattg gcccacctcc tatgattcgg
      361 aggacctcta ccgtccaaac gtaagtgtcc ggtttggatc ctattatttg cggaaaaatc
      421 gagatcagct cggtaataat ttatatgctg ccttggccgc ctacaatgcc ggacccggaa
      481 acgccattgt ctggagagat ctggcagggg atgaccctga tctactgctg gagatcatac
      541 ggttccagga aacacgcgat tacatccggt ttatttctga gatattcaac acctatcgga
      601 cgttttatag ccctgtccaa taaagaataa caggcaagcc ttgcaagaaa aaatccccga
      661 ttgaaacaat cggggattac tcattattta ttctattctt ttatcccata tgtgccacaa
      721 tgcgttgatg ggcggtttcc acatccatat cgagtactga gatcaatttg tcacggcccg
      781 attctccagc cacaatatcg aggcgggatt tcggtacgcc gagaatatcc gccagaaact
      841 cgatcagcgc ggtgttcgcc tcatcgtcca ccggtggcgc agcgatgcgc actttgatcg
      901 ttccatcatc cagcatttcg acgatttcat tgcggctggc acgcggcgtc acgcgcactg
      961 ccagagcaga gcctttttga ccgctatgca aattgaattt acgatcagtc ataaatcacc
     1021 tcaaggaaaa tagaaatgca acaagcaatc ggccaatcaa ttggagcaag aggatcaaaa
     1081 tcagtgggct aaaatcgaac attcccacca gtggcactat gcggcggatg ggatcaagca
     1141 taggctgaac aatgttatca accccgcgcc gcaccggatg atacggatcc ataaaaaagg
     1201 agaggatcgt agcaaagata acgagaatga cgagaatttg ttgaacggta ttgaccatta
     1261 tgataaggac atcgatcacg cttcacgctc cgacttgtcc acactgccat tgtgggacat
     1321 gctatgttgg tatgtgcgtg cacctacgat tgccgttcct actcgaacca gggtagcacc
     1381 ttcttccact gcaactgcgt aatccacact tgttcccatc gaaagttcac gccaatctgt
     1441 ttgaggaaat tgcatcgcca gacggtcgcg caacagcctg aggtgaatga aaaagggccg
     1501 tgaggcttcg gcatttgatc cgaggggcgg cattgtcatc aagccacgaa cggagaggtt
     1561 cggtaactcc aaaatggatt ggatctcagg aaacaacgtg ttccactttg attcctcgga
     1621 ggcattccat ccggatttac tttcctcgcc gcctgtgttg aattcaagta atacaggcag
     1681 tgtgcgatta ctttctgtcg caaaacgatt gaggcgctgc gcgagtttga gactatcgag
     1741 agaatgtaaa agtgcaaaat gatcaacgac aagccgagct ttacggctct gcacatgccc
     1801 gatcatgtgc cattctaccc cactttgccc gcgcaatgat tggatttttg ttacaccttc
     1861 ttcgggataa ttttcgccga ggatgcgcac gccagcttct atagcagctt ccgctacttc
     1921 cataggttgt gattttgtaa caacgacaag gcgcacgtcc tccggctgac gattgcttat
     1981 tcgcgcggcg cttgcgatgt gatcaagcgt agcctgataa ttctcacgaa tcgatttgac
     2041 caggtcagtc atggggtaat tataatattt cccctctccc gcttcgtctg caggacggaa
     2101 ccaccccgtc cttccgctca gcgcgaattg tttcgttcat tgcaatgaga ttaacgcacc
     2161 gaatcgaccg gtgcgaccct tttcagcgcg atgcgaaaac caatcctccg tgtgacaggc
     2221 cgtgcaaatc tctgcatgct cgatattgcc gacccctgcc cgttcgagca atagatgatt
     2281 cgccttccat aagtcaaaat ggatttttcc gttgtgtgat ttgagcaggg tttcagattc
     2341 gtctccatac ttttgcatca cctgaagaat tacgtctgca ccaatttgat aatgatccgg
     2401 tccgatcgaa ggtccgatcc ctgctgtgat atctgccgga ttagaatcat actgcttacg
     2461 catggcatcg acggttgcag tagccacgtc gtgtagagtt cctctccagc cggcgtgggc
     2521 taacccaacc actccctgac gagaatcatg gagcaaaatt ggaacacaat cagcaaagcg
     2581 catgaagagc gtcacttcgg gcgtatcggt aagaatgata tctgcctgac gtaacgactc
     2641 gtcgtgtggg cgaggcgcgc gtgcacagac cacgtccgcg ctgtggacct gccacacctc
     2701 aaacgccgtt tcaggggcgc gccccagcgc ataaaaagaa agcaagcggt tctctcttac
     2761 gcgctccagg tcatccccca ccgtacctcc cacatttagt gaactccagg gttccgggct
     2821 aactcctcca tgacgcgtga acacagcatg gcgcgtctgc aaggtatcaa attgaaaata
     2881 acgaagtccg ttgtgctgaa ttagaggcat gaaaccaata gtattactca ggaactattc
     2941 tgagagattg atgcaaactt cttgatgaaa aattgg
//
LOCUS       DASTJP010000899         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_139037, whole genome shotgun sequence.
ACCESSION   DASTJP010000899 DASTJP010000000
VERSION     DASTJP010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139037"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFQ23_26210"
     CDS             <1..803
                     /locus_tag="VFQ23_26210"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET9910166.1"
                     /translation="RVTSQMSQAASPTLAFVAAIEDAAELFRKAKRVVVLTGAGISSP
                     SGIPDFRSEGSGLWSRDEPMEVASLSVFRTAPERFFNWFRPLAGYIFHAKPNPAHLAL
                     AELEKAGRSLTVVTQNIDGLHQKAGSTHVVEMHGTLRTLSCTECFKQFESTPFLEPYI
                     TDGILPKCLSCNGILKPDVILFGEQLPQLAWLEAQRQTRQCDLMLVAGSSLEVLPVAG
                     LPMQALDRCAHLIVVNHTPTYVNVRADVVLMDDVSIVIPEIMKRVLHG"
     gene            796..2013
                     /locus_tag="VFQ23_26215"
     CDS             796..2013
                     /locus_tag="VFQ23_26215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9910167.1"
                     /translation="MAEIKTRTLDSYLRLIEISRDLASTLDLDTLLSDIVHAAADINS
                     AEAASILLYDDTSRQLYFQIATNIDEPTMRGLVVPLDKSIAGWIVTNRQSVRIDDAHN
                     DERFFKDVDQTIEYSTKSLLGIPLITKNKVVGVLEVVNKKRGKFTDADESMLTVLGAQ
                     AAVAIENARLFQQSDLISEFVHELRTPLASLSTATYLLLRPEMSREQSDQIVNNIHNE
                     TLRLNSLASSFLDLARLESGRVQFRKARFSVADLIYECRDVMMSKAQETSIQIRVDVP
                     PDMPLMEADRDKIKQILLNLTSNAIKYNRPNGSVIITGSFTDAELTITIQDTGLGIPD
                     DAIPHLFEKFYRVREHEGKASGTGLGLSICKQIIQGHNGRIEVKSKIGVGTSFSVHLP
                     RTTKTAQRSNESQ"
     gene            complement(2133..2504)
                     /locus_tag="VFQ23_26220"
     CDS             complement(2133..2504)
                     /locus_tag="VFQ23_26220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910168.1"
                     /translation="MKRRGIRIISEEEIERWRWDIVVEATGSPGGFSLARQAIRPRGT
                     LVLKSTYKGELSVNFSSIVVDEINILGSRCGPFEPALRLMESGQVDPTVLIMDEFEMG
                     NALKAFERAAEPGVLKVLVSP"
     gene            complement(2556..>3077)
                     /locus_tag="VFQ23_26225"
     CDS             complement(2556..>3077)
                     /locus_tag="VFQ23_26225"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9910169.1"
                     /translation="ISLRDVPPPSKPNEALVKIRKAGICSTDLELVKGYYPYTGILGH
                     EFVGEVVSLTPNSSPHGRGEGVNVGDRVVGEINAVCNACEQCLNGRPTHCENRTVLGI
                     VNRDGTFAEYTTLPIANLHRVPDSVPDEMAVFTEPLAAALEIQQQVQVSPTDRVLLVG
                     AGRLGQLIAQTLV"
BASE COUNT          767 a          854 c          742 g          714 t
ORIGIN      
        1 cgcgagtcac ttcccaaatg agtcaggctg ccagtccaac cctcgccttc gtcgcggcga
       61 tcgaggacgc ggcagaatta ttccgcaagg caaaacgcgt tgtcgtgctc accggcgcgg
      121 gcatttcttc tccatctggc ataccagact tccgttccga gggatcaggc ttatggtccc
      181 gcgatgagcc aatggaggtt gcctctcttt cggtgtttcg cactgccccc gaacgtttct
      241 tcaactggtt ccgtcctctg gcgggttata tcttccacgc aaagccaaat cctgcacacc
      301 tggcactcgc agaacttgaa aaggccggtc gctcactcac agttgtgaca caaaatatcg
      361 atggattgca tcaaaaggct ggttcaacgc atgttgtgga aatgcatggc acgctacgga
      421 cgttatcctg tacggaatgt ttcaagcaat ttgaatcgac ccctttttta gagccttata
      481 ttacggatgg catcctccca aagtgcttaa gttgcaatgg aattttgaaa ccagatgtga
      541 tattgttcgg ggaacaactc ccacaacttg catggcttga agcacaaaga caaacacgtc
      601 aatgtgacct gatgctcgtc gctggctctt ctctggaggt actgccggtg gcgggcctgc
      661 caatgcaggc tcttgaccga tgcgcacatc tgatcgtcgt caaccataca ccaacgtatg
      721 tcaacgtgcg agcagatgtt gtgctgatgg atgacgtttc catcgtaatc cctgaaataa
      781 tgaaacgagt gctccatggc tgagatcaaa acacgaacac ttgatagcta cctgcgttta
      841 attgaaattt cgcgcgacct ggcgtccact ctcgatttgg acacactgct ctctgacatc
      901 gtccacgcgg cagcagatat taattccgct gaagcggcct ctatcttgtt gtacgacgac
      961 acgtcgcgcc agctttactt ccaaattgca acgaacatcg atgaacccac catgcgcgga
     1021 ctggtggtgc cgctcgataa aagtattgcc ggctggatcg tcaccaaccg gcaatcggta
     1081 cgaatcgatg atgctcataa tgatgaacgt tttttcaaag atgtagacca aaccatagag
     1141 tattccacaa aatcgttgct gggcattcca ctgatcacca agaacaaggt cgtgggtgtg
     1201 ttggaagtag tcaataaaaa gagaggcaag ttcaccgatg ctgatgaatc gatgctcacc
     1261 gtattgggcg cacaggctgc cgtagctatc gaaaacgcgc gcctcttcca gcaatcggat
     1321 ctgatctcgg aatttgttca cgaattgcgc accccgcttg cctcactgag cacggctacc
     1381 tatctattgc ttcgccctga aatgtcgcgc gaacaaagcg accagatcgt caacaacatt
     1441 cataatgaaa cactgcgttt gaattcactc gcatcttcgt tcctggacct ggcacgcctg
     1501 gaatcagggc gtgtgcagtt tcgcaaggcg cgcttcagtg tggcagattt gatctacgaa
     1561 tgccgtgatg tcatgatgtc caaggcacag gagacgagta ttcagatccg cgttgatgtc
     1621 ccgcccgaca tgccactcat ggaagccgat cgcgataaga tcaaacagat cctgctcaac
     1681 ctgaccagca atgcgatcaa atataaccgt ccaaacgggt ctgtcattat cactggcagt
     1741 tttacagatg cagaactaac catcacgatt caggataccg gcttaggcat tcccgatgac
     1801 gccattcccc atctttttga aaaattctat cgagtacgcg aacatgaagg aaaggcgtcc
     1861 ggcaccgggc tgggcctttc gatttgcaaa cagatcattc agggacacaa tggccgcatt
     1921 gaagtaaaga gcaaaatagg ggttgggaca tcgtttagcg ttcacctgcc gcgcaccacg
     1981 aagactgcgc aacgcagtaa cgaatcacag taacatctgt cgttgcgagc gaagcgaagc
     2041 aatcccctaa caactctgag attgctcacc tgcactgcac caagcacagt gcggtgcaag
     2101 tgtcgtcgcg acaaacgctc ctcgcaatga cattatggac tcaccaaaac tttgagcacg
     2161 ccaggttctg cggcgcgctc aaatgctttt aaagcattcc ccatctcaaa ctcgtccatg
     2221 atcaatacag tcggatccac ctgcccggat tccatcagac gcaaggcagg ctcaaaggga
     2281 ccacaacgcg agcccaggat attgatctcg tccacaacaa tcgacgagaa attgacgctg
     2341 agttcacctt tataagtgga tttaagcacc agcgtcccgc gcggacgaat cgcctgccgc
     2401 gccaaagaaa aaccgcccgg cgacccggtc gcttccacga cgatatccca tctccaccgt
     2461 tcgatctcct cttcagaaat gattctgatg ccgcgtcgct tcaatagatc ctgctggcgg
     2521 ctatggcgtg caacgacgcg caggtcgcag cctgtctata ccagggtttg agcgatgagt
     2581 tgtcccagcc gccccgcgcc caccagcaag actcgatctg tagggctgac ctgtacctgc
     2641 tgttgtatct ccaatgccgc cgcaaggggc tccgtgaata cagccatttc atctggaact
     2701 gaatcgggca cgcgatgtaa gttggctatt ggtaaggtcg tatattctgc aaatgtgccg
     2761 tcacgattaa caatccccag tacggtgcga ttctcacaat gagttgggcg tccgttcaaa
     2821 cattgttcac acgcgttgca aaccgcgttg atctcaccca cgacgcggtc accgacattt
     2881 acgccttctc ctctcccatg gggagaggag ttaggggtaa gggagaccac ctcaccgaca
     2941 aactcatgac ccagaatgcc cgtgtatggg tagtagcctt tgactagttc aaggtctgta
     3001 ctgcaaatcc ctgctttgcg aattttgacc aatgcctcat tcggtttgct cggtggaggg
     3061 acatcgcgta aagaaat
//
LOCUS       DASTJP010000900         7364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_89815, whole genome shotgun sequence.
ACCESSION   DASTJP010000900 DASTJP010000000
VERSION     DASTJP010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89815"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFQ23_26230"
     CDS             complement(<1..651)
                     /locus_tag="VFQ23_26230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910170.1"
                     /translation="MRSLEISSRRETWLSVALITLVTLLTNASLISQLGFYRDDWYLM
                     WVAQYQGSEGIIALFRGDRPLFGWFYALDYLLLGDAPLGWHLLGLVIKLITALGTLWL
                     LRSLWPQHKLETTLMTLLYVVYPGFYQQPNIGTFMNHFMAYAAAILSLACTVQALRAT
                     RFSSKAIYAFFGLILAGFYIFTYEALIGTEAVRLLLIGYFYYRLNGSSWQSIIRKTL"
     gene            complement(651..1787)
                     /gene="rffA"
                     /locus_tag="VFQ23_26235"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(651..1787)
                     /gene="rffA"
                     /locus_tag="VFQ23_26235"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HET9910171.1"
                     /translation="MELRVDFNRPSTVGNEFEYMRQAIENVHISGDGPFTKKCHELLE
                     RELGVPKALLTTSCTHALEMSAILLDIQPGDEVIIPDFTFVSTVNAFVLRGATPRFID
                     VRPDTLNLDESRLEALITPRTKAILPVHYAGVGCEMDSIMEIANRHNIPVVEDNAHGL
                     FGKYKGKFLGTFGCMASQSFHETKNFTSGEGGALLINDRDLIERAEIIREKGTNRSRF
                     FRGQVDKYTWVDIGSSYLPSDMLAAFLYAQLEQREKIQLHRKQIWEIYFAGLQDWAAA
                     HNVSLPHIPAHCEQAYHMFYMLLPTLELRQKMISYLRERGIYSVFHYLPLHLSDMGQR
                     FGGKEGDYPVTESISDQLLRLPFHNALTSNEQEIVIEAIREFPF"
     gene            complement(1778..2731)
                     /locus_tag="VFQ23_26240"
     CDS             complement(1778..2731)
                     /locus_tag="VFQ23_26240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910172.1"
                     /translation="MQIIQAPRASAILYTLLVSQPAPRPWILPANICPIVPITFFKAR
                     VPFEFVDIAADTLHMDLEQAETLIKSRHYGGLLYAHSYGEPSTPNEFFLHAKSIHPDL
                     LILDDRCLCIPALDPNANSPADLTLYSTGYAKIVELNFGGYAFLKENIPYKPVHLPFH
                     PQDYEKLENSYKKSISERAMFSYHDTNWLDTISNLPAWYDYRQKIQSHMDDSLAHRTT
                     LNKIYSSQLPREIQLPNAYQSWRFNLRVKNKKRILDAIFKKGLFASSHYASLAGTMAD
                     GNAPQAEMLADEVINLFNDKHFTVEQANQVCEIITENLSWN"
     gene            complement(2811..3446)
                     /locus_tag="VFQ23_26245"
     CDS             complement(2811..3446)
                     /locus_tag="VFQ23_26245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9910173.1"
                     /translation="MTIDETQKKLNDYFTEKLETFGATAKGVDYNGEQARQTRFAELV
                     KIINPSKPFSIIDYGCGYGAMYEYLHEKGWDFEYYGVDLIEKMVTAGREKYAGFSNAH
                     FTTDEQELPVADYLVAAGIFNIKLESSYGEWQNFICETLPRMNALCSKGFSFNMLTKY
                     SDADRMAERPDLFYGDPLFFFDFCKRNFSRNVALLHDYGLYDFTILVRKDL"
     gene            complement(3443..4135)
                     /locus_tag="VFQ23_26250"
     CDS             complement(3443..4135)
                     /locus_tag="VFQ23_26250"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HET9910174.1"
                     /translation="MKVGIIQSNFLPWRGYFDFIRETDLFIVHDDLQYTKGDWRNRNK
                     IKTPRGVEWITVPVHYHHTGQLIEETRIDYSTPWVPKMLNRIRESYRRSPCFETYFSE
                     LADLLNASAPSISDLNLRLIKWVCSRLEIDTPIQLSRDYNPKGTKTERLIGILQDAKA
                     TAYLSGPAAQVYLVPELFEHAGIQLEYKQYEYPEYDQLYPPFESKVSVIDLLFMKGKE
                     AIAYMENKEKQA"
     gene            complement(4132..5055)
                     /locus_tag="VFQ23_26255"
     CDS             complement(4132..5055)
                     /locus_tag="VFQ23_26255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9910175.1"
                     /translation="MNVSVVIPVYRSEHTLDTLIERLGNVLPTIADSYEVLLVNDGSP
                     DNSWDSIERLAKSHYWVRGIDLMRNYGQHNATLCGIRAARYEIIFTMDDDLQNPPEEM
                     QKLLTKLNEGYDVVYGVARRRQQAWWKNLFSVLVKRAIGYVMGLRTVRDIGAYKAFRS
                     HLLKAFEDFNGPDVLVDVLLSWGTNRFAAVTVEESPRTVGQSNYNLLRLIKVSLLVLT
                     SYTTVPLRLASILGFFFTVVGFFILSYVVITYFVAGSVPGFSFQASIIALFSGVQLFS
                     LGIIGEYLARVFERTGGRKTYIVGRTTDDVK"
     gene            complement(5086..6804)
                     /locus_tag="VFQ23_26260"
     CDS             complement(5086..6804)
                     /locus_tag="VFQ23_26260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET9910176.1"
                     /translation="MNRNEALSSLKENPDISVLIIGGGINGIGTFRDLALNDVDVLLV
                     ERGDFCSGASSASSHMAHGGIRYLENGEFRLVREAVQERNRLIQNAPHIVKPLPTAIP
                     IFKWFSGLLNAPLKFLGVLDKPSERGSVVIKVGLMLYDAYSRVQAGKGGQSAQRAVPK
                     HQFLSRTQSLKKWNELNPDIVNTATYYDGAILKPERLGVELVLDAEAANPNAHALNYM
                     TMVGGSEDTIILRDEFTDESYDVRPKLVINATGSWIDFANRKLGLSTRFIGGTKGSHI
                     VLKHDELRNAIGDHEFYFENKDGRLVLIYPLFDCVLVGTTDVPLENPDEARCTDEDVN
                     YFLDMIQRVFPRIKLSPEDVVFRYSGVRPLPRSSAKSTSQVSRDHSIEVVSGDWTNLS
                     FPVYSLVGGKWTSFRAFSEQTTDKVLAFLNLQRTKHTRDLPIGGGHGYPRDPAELKRL
                     IESLSAWTGVTRERLKMLFERYGTRAEAVATYMNGGTDFFLKSLPDHSRREIAFLVQH
                     EKIFHLDDFVLRRSMLAMLGHLNREIIDELAEVFSNTLGWDIDQRKAEVARTLFILED
                     RHGVRL"
     gene            complement(6851..>7364)
                     /locus_tag="VFQ23_26265"
     CDS             complement(6851..>7364)
                     /locus_tag="VFQ23_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET9910177.1"
                     /translation="VEELARSVEDNGGIYFVPAFSGLFAPYWKSDARGAIVGMTRYVN
                     KGHIARAALEATAYQTREVLDAMEADSGVTLTALKVDGGMVFNELLMQFQSDILNVPV
                     VRPKVAETTALGAAYAAGLAVGFWKDFDELKNNWGVDKEWRPAMDADERMKLYSSWKK
                     AVTRTFNWTD"
BASE COUNT         2001 a         1824 c         1728 g         1811 t
ORIGIN      
        1 tagtgtctta cgaataatcg attgccaact ggagccgttt agcctgtagt aaaaatatcc
       61 gatcagtaaa agcctaaccg cctccgttcc aatgagagct tcataggtaa atatgtagaa
      121 gcccgccaga attaagccaa aaaatgcata gattgcctta ctcgaaaaac gagtcgccct
      181 tagggcttgc accgtacaag ccaatgacaa aattgccgcg gcatacgcca tgaaatgatt
      241 catgaatgtc ccgatattgg gttgttgata gaaaccaggg tagaccacgt acaaaagtgt
      301 catcaatgtg gtttcaagct tgtgctgtgg ccacaggctt cgcagtaacc acagcgtccc
      361 taaggcagtg atcaacttaa tgaccagtcc caaaagatgc cagccaaggg gtgcatctcc
      421 cagcaataaa taatccagcg cgtaaaacca accaaagaga ggccggtcgc ctctaaataa
      481 agcaataatg ccttctgagc cctgatactg tgccacccac atcagatacc agtcatcgcg
      541 atagaaaccc aattgcgaga tcaaagaagc attggttaga agagtgacca aagtgatgag
      601 tgccactgaa agccaggttt cacggcgaga tgagatttcc agcgatcgca tcaaaaagga
      661 aactcccgaa tggcttcgat cacaatttcc tgttcgttac tggtcagcgc attgtggaat
      721 gggagacgta acagctggtc actgatgctt tcggttactg gataatcccc ctccttgcca
      781 ccaaatctct gacccatatc tgaaaggtgc aaaggcagat aatgaaagac gctgtaaatt
      841 ccacgctcgc gtaagtaaga gatcattttt tgtcgcagtt ccagggttgg caacagcata
      901 tagaacatgt gataggcctg ttcacaatga gcagggatat gaggcaggct tacattatgt
      961 gcagcagccc aatcctggag ccctgcaaaa taaatctccc aaatctgttt acgatgcaat
     1021 tgtattttct cacgttgttc cagctgggca taaaggaagg ccgcgagcat gtccgacggc
     1081 aggtacgaag aaccaatatc aacccaggta tatttatcca cttgcccgcg gaagaaccgc
     1141 gagcgattcg tccctttttc tcggataatt tctgcacgct ctattaaatc tcgatcattg
     1201 atgagaagcg caccgccctc tccactggta aagttttttg tctcgtgaaa cgattgagac
     1261 gccatacagc caaacgtgcc aagaaatttc cccttatact ttccgaacaa tccgtgcgcg
     1321 ttgtcttcga ctaccgggat gttatggcga ttcgcaatct ccatgatgga gtccatttca
     1381 cagccgacgc cagcataatg cacagggagg atggcctttg tgcggggagt gatcaacgct
     1441 tccagcctgg attcatccaa attaagagta tcgggtcgaa cgtcgatgaa acgaggcgtg
     1501 gcgccacgta gaacgaaagc attcaccgtc gagacaaagg taaagtccgg gataataact
     1561 tcatcgccgg gctgaatatc caataaaatg gcagacatct ccagcgcgtg cgtgcaggag
     1621 gtagttagta aagctttcgg tacgccaagc tcacgctcca gcaattcgtg acatttcttc
     1681 gtaaacggac catccccgga gatgtgcacg ttttcgattg cctgacgcat atactcgaat
     1741 tcgtttccaa cggtgctcgg acggttgaaa tcaactctta gttccaagag agattctccg
     1801 tgatgatctc acaaacctgg ttcgcctgtt caacggtaaa gtgcttgtca ttaaataggt
     1861 taattacttc atccgcgagc atttccgctt gcggagcatt accatctgcc attgttccag
     1921 ctaaggaagc atagtgggag ctggcgaaca atcctttttt gaaaatcgca tccagaatac
     1981 gctttttgtt cttcacgcga agattgaatc gccagctttg gtaggcatta ggcaactgga
     2041 tctcacgtgg taattgactg gagtaaattt tgttcaaggt cgtgcgatgt gcgagagagt
     2101 cgtccatgtg gctctgaatt ttctggcgat aatcatacca tgcaggcaaa ttggatatcg
     2161 tatccagcca gtttgtatcg tgatagctaa acatcgcacg ttctgaaatc gactttttgt
     2221 aggaattttc cagcttctcg tagtcttgag ggtgaaatgg caggtgaacc ggtttatatg
     2281 gaatattttc ttttagaaac gcataaccgc caaaattcaa ctcgacgatc ttggcgtagc
     2341 cggtgctata taaagtgagg tcagcgggcg aattggcatt tgggtcaagc gccgggatac
     2401 acagacagcg atcatccaga attagcagat caggatggat agatttcgca tgtaggaaaa
     2461 attcatttgg cgtagatggt tcgccgtagg aatgtgcata tagtaatccc ccatagtgac
     2521 gcgactttat taacgtttcg gcctgttcaa gatccatgtg cagcgtatcg gccgcgatat
     2581 ctacaaattc aaacgggacg cgtgccttga agaatgtgat cgggacgatt gggcaaatgt
     2641 tagcaggcaa tatccacgga cgaggcgcag gttgggaaac aagaagggta tatagaattg
     2701 cagaggcccg aggggcttgg atgatttgca tgttcgtagt aaagcaattc ttctattcga
     2761 gaagattgct tcgtcgtggc ttcgccactc ctcgcaatga cagttgaaat ctaaagatcc
     2821 ttgcgaacaa ggatcgtgaa atcgtacaga ccgtaatcat gcaggagtgc cacattacgc
     2881 gaaaagtttc gtttgcagaa atcaaaaaag aatagcgggt cgccgtaaaa aaggtcaggg
     2941 cgttctgcca ttcggtctgc gtctgagtat ttcgtcagca tattgaacga aaacccttta
     3001 gaacagaggg cattcatacg aggtaaggtc tcacaaataa aattctgcca ctcgccgtac
     3061 gatgattcaa gttttatgtt gaagatgcct gctgccacca gataatcagc cacaggcagt
     3121 tcttgctcat cggttgtgaa gtgcgcattt gaaaaacccg cgtatttttc acgtcctgca
     3181 gtgaccatct tctcaatcaa atctacaccg taatactcaa aatcccaccc cttttcatgg
     3241 agatactcgt acatagcgcc gtatccacag ccgtaatcaa taatcgaaaa tggcttggac
     3301 gggttgataa tctttaccaa ctcggcaaag cgcgtctggc gagcttgctc accgttgtaa
     3361 tccacaccct ttgcggtagc gccaaaggtt tcaagctttt ccgtaaagta gtcgttgagt
     3421 ttcttttgtg tttcgtcgat ggtcatgcct gcttttcctt attttccatg tacgcgattg
     3481 cttcttttcc cttcataaag agcaggtcga tcacactgac ctttgattca aatggcgggt
     3541 agagctggtc atattccgga tactcatact gcttatattc caattggata ccggcgtgct
     3601 caaataattc tggaaccaaa tagacctgtg cagccgggcc cgatagatat gcagtcgcct
     3661 tcgcgtcttg aagaattcct atcaaccttt cggttttcgt acccttcgga ttgtaatcac
     3721 gagataactg gatgggtgta tcaatttcaa gcctggagca aacccactta atgagtctca
     3781 ggttcagatc cgagatggat ggggcggacg catttagcaa atccgccagt tctgagaaat
     3841 aagtttcaaa gcatggagag cgccggtagg actcacgaat ccggttcaac attttcggca
     3901 cccagggcgt ggagtaatct attcgtgtct cttcaattaa ttggccggta tggtgataat
     3961 gaacgggaac cgtgatccac tctacaccgc ggggagtctt gatcttgtta cgattgcgcc
     4021 agtcaccctt ggtatattgc aaatcatcat gaacaatgaa taaatcggtc tcacgaataa
     4081 aatcaaaata accgcgccac ggcagaaaat tcgattggat aataccaact ttcatttgac
     4141 atcatccgtg gttcgcccaa caatgtatgt tttacgaccg cccgtgcgct cgaagacccg
     4201 cgctagatat tcaccgataa tgcccaggga gaacagttgc acgccgctaa aaagtgcgat
     4261 gatggaggcc tgaaacgaaa agcctggcac ggatcccgcg acaaaatagg tgatgaccac
     4321 gtaggacaaa atgaaaaatc cgacgactgt gaagaaaaac ccgagtatgc tcgccagccg
     4381 cagcggaacg gtggtgtaac tggtcagcac cagtaaagaa actttgatca atcgaagaag
     4441 gttatagttc gattgcccca ctgtccgagg tgattcctcg acggtaacgg cggcaaaacg
     4501 gtttgtaccc catgaaagca gaacatccac taagacatct ggaccgttga aatcttcaaa
     4561 ggctttaaga agatgagagc gaaacgcctt gtatgccccg atatcacgta cggtacggag
     4621 ccccatgaca tagccgatgg cacgcttcac caatacagaa aacagattct tccaccaggc
     4681 ctgttgacgc ctgcgcgcca cgccatacac aacatcatag ccctcgttca attttgtcag
     4741 cagcttctgc atctcctcag gcggattttg caaatcatca tccatggtaa agatgatctc
     4801 atagcgcgcg gcacgaatcc cacacagggt tgcattgtgt tgaccataat tgcgcatcag
     4861 gtcgatgccg cgcacccagt aatgagactt agccagtcgc tcaattgaat cccagctgtt
     4921 atcgggactg ccatcattaa ccagcaaaac ttcataggaa tcagcgatcg tcggcaacac
     4981 gttccccagc cgctctatca atgtatccag agtatgttca ctgcgataca ctggaatgac
     5041 aacggataca ttcatacggg acgtaattct accacccgcc tcacatcaaa gccggacgcc
     5101 gtgcctgtcc tccaaaataa acaaggtgcg cgccacttcg gcctttcgct gatcaatatc
     5161 ccagcctaag gtattgctaa atacctccgc gagttcatcg atgatttcgc gattaagatg
     5221 ccccagcatg gccaacatgg atcgcctcaa tacaaagtca tcgaggtgga agatcttttc
     5281 atgctgcacc aggaatgcga tctcgcgccg tgagtgatcg ggcagtgatt tgagaaaaaa
     5341 gtcggtgccg ccgttcatat acgttgcaac cgcttcagca cgagtgccat aacgctcgaa
     5401 caacatcttc aatcgctcgc gggtaacccc cgtccatgca gagaggcttt cgatcaggcg
     5461 ttttaattcg gcgggatcac gcggatagcc atgaccaccc ccgattggta aatcacgggt
     5521 atgtttggta cgctgcagat tgagaaaggc cagcacttta tcggtggtct gttcggagaa
     5581 ggcacggaac gaagtccatt tgccgcccac cagggaatat acaggaaaag acaaattggt
     5641 ccagtccccg ctgacgactt caatgctatg atcgcgagag acttgagatg tggatttggc
     5701 agaagagcga ggcagcggac gcacgcccga atatcgaaac acgacatctt ccggagagag
     5761 cttgatgcgt ggaaagacgc gctggatcat atccagaaag taattcacat cttcatccgt
     5821 acagcgtgct tcatctgggt tctcaagtgg gacatcagtc gtgccaacca gcacacagtc
     5881 aaacaatgga taaatcagca caagacgacc atctttattc tcgaaataaa attcatgatc
     5941 gccgatggca tttcgcaatt catcatgttt caaaacaata tgggagcctt tggttccgcc
     6001 aataaaacgg gtagaaagtc caagcttacg attggcaaaa tcgatccatg aaccggtggc
     6061 gttgatcaca agctttgggc gcacatcata tgattcatcc gtgaattcat cccgaagaat
     6121 gatggtatct tcgctgccac ctaccatcgt catgtaattg agcgcgtgcg cattcgggtt
     6181 cgcggcttct gcatccagca ctaactccac tcccagacgt tcaggtttca gaatggctcc
     6241 atcataatac gtggctgtat tcacaatgtc agggtttaat tcattccact ttttaaggga
     6301 ctgggttcgc gaaaggaact gatgctttgg aacagcgcgt tgcgcgcttt gcccaccctt
     6361 ccccgcctgt acgcgggaat acgcgtcata cagcattaac ccgaccttga tcaccacaga
     6421 gccgcgttcc gaaggtttat ccagcactcc aagaaatttc agtggggcat tgagcagacc
     6481 ggagaaccat ttgaaaatgg gaatggcagt tggaagtggt ttgacaatat gcggtgcatt
     6541 ttgtatcaaa cggttgcgtt cctgcacagc ctcgcgcacg agacgaaact ctccattctc
     6601 cagatagcga ataccaccgt gagccatgtg agaggaagct gaggaggcgc ccgaacaaaa
     6661 atcaccgcgc tcgaccagta aaacatccac gtcattgagc gcgagatcac gaaatgtgcc
     6721 gatcccgttg atgccgccgc caatgatcag gactgaaatg tcagggtttt ctttgagaga
     6781 agaaagagct tcgttgcgat tcattttatc ccaacatgag ggtcattacg ttcaaaagga
     6841 ttctgtgtgt ttagtccgtc cagttgaatg tccgcgtgac tgcttttttc cagcttgagt
     6901 ataacttcat tcgttcgtcc gcgtccattg ctggccgcca ttctttgtcg acgccccagt
     6961 tgtttttcaa ctcatcgaaa tctttccaaa aaccgacagc taacccggca gcataggctg
     7021 cacccagtgc ggtcgtttca gcaaccttcg ggcgaacaac tggtacattc aaaatatcag
     7081 attggaattg catcagcaat tcattgaata ccatgcctcc atccactttg agcgcagtca
     7141 acgttacacc tgaatccgct tccattgcgt ccaatacttc gcgcgtctga tatgctgtcg
     7201 cttcgagcgc ggcacgggcg atatgccctt tgttgacata acgagtcatt ccgacaatcg
     7261 caccacgcgc gtcggacttc caatatgggg cgaacaatcc cgaaaacgcg gggacgaaat
     7321 atataccacc attatcttca acggatcttg ctaactcttc cacc
//
LOCUS       DASTJP010000901         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_617712, whole genome shotgun sequence.
ACCESSION   DASTJP010000901 DASTJP010000000
VERSION     DASTJP010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617712"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFQ23_26270"
     CDS             complement(<1..456)
                     /locus_tag="VFQ23_26270"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET9910178.1"
                     /translation="MTTHRERIQAAIKGEIVDHPPVALWRHFPVDDQSPETLANAALH
                     FQENYDFDLVKVTPASSFCVKDWGVQDEWVGDTEGTRRYTQRVINDPRDWESLPILDS
                     TAPHLSAQLECLRLIRRALGPDTPLLQTVFSPLSQAKNLAGNDLLLEHLR"
     gene            complement(453..2039)
                     /locus_tag="VFQ23_26275"
     CDS             complement(453..2039)
                     /locus_tag="VFQ23_26275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910179.1"
                     /translation="MNFSTQRILKKPILFLLAVNVLIGLFVFRDYGLSWDEPLFYDYA
                     DALGYAYSPREWFSGNFDLNNSFGSSGDDHKTRGPAYLFLARGPAYLLEAFGLDSASA
                     WHLVNFLFFQLGVYFLYRLSRRWMKPGAALAASALFSWQPLLWGHAFINPKDPPFLVF
                     FLGSICLGFEMVDQLFADIKNRQKFISAIIPAILLGMTTSIRVLGPLAALLVFVYFVI
                     RVMQEKKLRFPASQWLTFLAYGFIAIVTMFITWPYLWENPLARFFEVFRLMSDNPTTL
                     SVLFGGEIYRAGELPRRYFPFMLLTTLTEPVWILFGVGLVAGYWKLVADPSPEKINKL
                     TTITLTLFWFLFLLAYVLIRRPSMYDGIRHFLFILPPIFIFAGFTFEYLARLNASFWL
                     RAGAAIVLLPGLISIIKLHPYEYTYYNSIVGGVSGAFRNYETDYWLTCYKESVEQLEA
                     LGEPINLFVHREAYIAEYYADAETSVRELRGALSDVQSGDYVLVNTRTNEDRRVFKDA
                     PTVLQISRSGAEFCIIKRVP"
BASE COUNT          618 a          489 c          536 g          446 t
ORIGIN      
        1 acgcaaatgt tcaagcaaga gatcattccc cgccagattc ttggcctgcg ataaaggact
       61 aaaaacagtc tggagcaggg gcgtgtctgg gccgagcgcc cggcggatga ggcgaaggca
      121 ttccaattgt gcagagaggt ggggggcagt cgagtcgagg atcggcaaac tttcccagtc
      181 acgcggatcg ttgatcacgc gctgtgtata tcgtcttgtc ccttccgtat cgccgaccca
      241 ttcatcctgc acgccccaat cttttacgca aaacgaagac gccggtgtga ctttcaccag
      301 atcaaaatca taattttcct ggaagtgcag ggccgcattc gccaaagtct caggagattg
      361 atcatccacc gggaagtgac gccataaagc gacgggcggg tgatctacga tctcaccttt
      421 gatggcagcc tggatacgtt cacgatgtgt ggtcatggga ctctcttgat gatacaaaat
      481 tctgctccac tacgactgat ctgaagcaca gtgggcgcgt ctttaaatac tctgcggtct
      541 tcgtttgtgc gcgtattgac aaggacataa tcaccagact gcacgtcgct caacgcgccg
      601 cgcaactcac ggacgcttgt ttcggcgtct gcataatact cagcaatata ggcttcgcgg
      661 tgaacaaaaa ggtttatggg ttcaccaaga gcctcgagct gctccaccga ttctttatag
      721 caggtgagcc aatagtcggt ttcataattt cgaaaagcac cgctcacgcc gccgacgatt
      781 gaattgtaat aggtatattc atagggatga agttttatga tactaatcaa gccggggagt
      841 aaaacaatag ccgcacctgc gcgtagccaa aacgaggcgt ttaacctggc tagatattca
      901 aatgtgaaac ctgcaaaaat aaaaatggga ggaagaatga agaggaagtg cctgatgcca
      961 tcatacatcg aaggtcggcg gatcaacacg taggcaagga gaaaaaggaa ccaaaatagc
     1021 gtcagcgtga tggttgttag tttattgatt ttctccggtg atgggtcggc aactagtttc
     1081 caatagccag cgacaagacc tacaccaaag aggatccata ctggctcggt caacgttgtc
     1141 agcagcatga aagggaagta tcgcctgggc agttcacctg ctcgataaat ttcaccaccg
     1201 aatagaaccg aaagcgtcgt gggattatca gacattaacc gaaagacttc aaaaaagcgg
     1261 gcgaggggat tctcccacag atagggccat gtaatgaaca tggtcacgat cgcgataaat
     1321 ccgtaggcaa ggaatgtcag ccactgggag gcagggaagc gaagcttttt ttcttgcata
     1381 actctgatga caaaataaac aaaaaccagc agtgcagcca gtggacctag cacacggatc
     1441 gacgttgtca tgccaagtag aatggccggg atgatcgcgc tgataaactt ttgtctgttt
     1501 tttatgtcag cgaaaagctg atctaccatc tcaaaaccaa ggcagatcga gccaagaaag
     1561 aaaacaagaa agggtggatc cttcgggttg ataaaagcgt gcccccaaag aagcggctgc
     1621 caggaaaata acgcggaggc ggccagagcc gcacccggtt tcatccagcg cctggataaa
     1681 cgatacagaa aatagacccc aagctgaaaa aaaaggaagt tgacgaggtg ccaggcagag
     1741 gctgagtcca gaccgaatgc ctcgagtaag taagcaggtc ccctggcgag aaacaagtaa
     1801 gccggaccac gcgtcttatg atcatcccca ctactgccga acgaattgtt taagtcgaag
     1861 tttccgctga accattcccg tggggaatat gcatatccaa gcgcatcggc ataatcgtaa
     1921 aataagggct catcccagga aagtccgtaa tcacggaaaa caaatagccc gatcaaaacg
     1981 ttgacggcta ataagaaaag aataggtttt ttaagtattc gttgggttga aaagttcata
     2041 acagctggtt accgacgata tcaaaggcta tgatttcagc cgaagcgca
//
LOCUS       DASTJP010000902        11218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_574795, whole genome shotgun sequence.
ACCESSION   DASTJP010000902 DASTJP010000000
VERSION     DASTJP010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574795"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1303
                     /locus_tag="VFQ23_26280"
     CDS             116..1303
                     /locus_tag="VFQ23_26280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9910180.1"
                     /translation="MKIALLTEKYTPDIGGLAISTERFARLLSSAAHEVHVFAATSNL
                     SPSEKRTLSHDQIHVTRFGAHKRVDDTLVDWFELVIEEHHRNPFDVLHAYFLTQAGFI
                     ATYAGRYLNIPSVVSIRGNDIERAAFDPSKFSHVMFALQNASAVTANAKELVDRTRAF
                     VDREIVLIPNGIDTDHFKPSEKIPKLVQTLRLIEASSLPFSSWTTQTSPFHHRAIGFA
                     GELREKKGLKTLLAAYAQVCKKHSSTSLLIVGVVRPGGDKEYFDDFRTRNPNLQIIVT
                     GHISPKGLPNYYALMDVFVHPSLRDGMPNALLEAMACGKPVVTTPVGGMLDVIKDGEN
                     GIFVPVNDSEALSNAIEELLNNAERRNQLGTAARQTILDGFSPQKELEANLAVYRKLG
                     LNP"
     gene            1359..3188
                     /locus_tag="VFQ23_26285"
     CDS             1359..3188
                     /locus_tag="VFQ23_26285"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET9910181.1"
                     /translation="MSDLSGKTLGQYQIIKPIGQGGMATIYLAQQPSVNREVAIKVLA
                     APLQENASFVKRFNQEVEVIAHLQHPQIIPVYDFGDRGDEIYIVMAYMRGGTLAERIE
                     KAPKGLPDGETIRLLDLIADGLDFAHNKNIVHRDLKSNNILMDEDDHPYIADFGLAKL
                     TEGKIELTNTMMTGTAAYMAPEIAQSGKSTKRADIYALGIMVFEMLTGRLPFEGETPY
                     KMLSAHIHDPVPNVRKFRRDVPPEVQPVLERALAKNPVNRFATAPEFAAALKDALKTT
                     SPEAGYQWLLKPREFFRRSPRLLLVSVTAILLAGSIFFTSRLSNNAELPSAVVDPTKH
                     IARAITDASGVTTWTFGCLGNSNSALVDLDCREIRVAVENRILPYNYIFLETNQPGGM
                     DYDMWWEICTLLHCQPVFIEEKWETLLDEVSKGNYDSASEGITITQARREILDFSISY
                     LNIEQRLVVRKGESRFSNINEFLANENLIAGALEDSTNFDAAREYIPEDRVKTYKEFS
                     TVFYALATGDIDAAIADQAPGQTTVTGIELQQAINLEFIGTSLSSDQFGVVFPKGSDL
                     VDPINEALQNLRAQGILDELIKRYFGPGFTVTYNDIDLGTHGQ"
     gene            3267..4223
                     /locus_tag="VFQ23_26290"
     CDS             3267..4223
                     /locus_tag="VFQ23_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9910182.1"
                     /translation="MYNPFESTNTVKSSLNGQKYIASNEIATIVYLAQKLGKPLLTEG
                     PAGVGKTELAKAIAAATGRDLIRLQCYEGLDESKALYEWEYSKQLLYTQLLRDKLNDT
                     LGKAESLAEAADKLAKEEDVFFSNRFLLQRPLLKAIMSDQPSVLLIDEIDRADAEFEA
                     FLLEVLSDFQVSVPELGTLTAKHRPFVVLTSNNTRELSEALKRRCLYLFIDYPTLQEE
                     LAVVRLKVPDLNPKLAQQAVEFVQKLRKGDMRKSPSISETLDWANALVALNAKSLDKD
                     TLEDTVSILLKHEADLQKAKRQYINPPPPRQRPEDFGKFSGN"
     gene            4235..4636
                     /locus_tag="VFQ23_26295"
     CDS             4235..4636
                     /locus_tag="VFQ23_26295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910183.1"
                     /translation="MLNPEFASSSTFGILFLIFVLSNMVLMHLIFVTYDSLLKLEYNF
                     YRMSWESDGQPFGFFGIDAKDQSLQISLPAFLRKQYDQAVLSIKWLFSTPSWMVNDIQ
                     ALQLVSKYRWLMLIWIIFGAIPTFVLIITVF"
     gene            4665..5558
                     /locus_tag="VFQ23_26300"
     CDS             4665..5558
                     /locus_tag="VFQ23_26300"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9910184.1"
                     /translation="MDNPHNFKRPTSVKEMQERFENFATDEGWQHGLNYKPDPTDIFI
                     VTPAKCGTTWMQQIVHGLRTRGSMDFDEISRVVPWINMAYDVGIDIYAPQVARPHAFK
                     THTRLDEVPKGGKYIVIVRDPGDALVSAYHFMEEMFFEKGTITLEEFAHEEFLPNREI
                     HTHTLAFWDRRNDDDVLPLAYENMKSNLTRTIEQVAAFMDIPLDEELREIVLRQSDIK
                     FMQEHKEQFEDHLIRKARSEAMRLPQDGQLNKVRNGQVGESRERVPEEIIKELDVLWQ
                     EVVTPKTGLHSYEDLRKELDR"
     gene            5555..6343
                     /locus_tag="VFQ23_26305"
     CDS             5555..6343
                     /locus_tag="VFQ23_26305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910185.1"
                     /translation="MTVRNELTKSESLLFSVEERNRIRDRVLEIASSDGRIVSGAVVG
                     SMALSEGDRFSDLDLTFGVVDEFSIDNVLEDWTRQIIEEFDAAQLFDVASGASIYRVF
                     LLPGCLQFDLSFTPASKFGAIGPKFKLLFGDAVEKPYLPTQSAAELFGYAVHHALRAR
                     FCIERGRYWQAEYWISSLRDYALSIACHRHSLPVSYARGFDHLPAEVLEPFKSGLVAS
                     LERLELLRALKVVIDGLIRESVEVQEIGNKVEPQLRLLTREWNI"
     gene            6361..7737
                     /locus_tag="VFQ23_26310"
     CDS             6361..7737
                     /locus_tag="VFQ23_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9910186.1"
                     /translation="MESRILQLISALRASGVRVSLAESAEAFSAVDLMGIQDREQFRL
                     SLRATLIKDVRDLPVFDKLFPLFFGTGTPPMMGGNPTDDMTPEEAQMLADALRQFAQN
                     LRQRMERLMNGEPLTKSELEELGQMVGLDQTDDLRYQNWMAQRMMRALEYPEVREAMR
                     ELMEQLEQMGMSKERVDDLREQIRQNLQGMQEQINQFAGQRIAENLSQQPPSDGVDNL
                     MNRPFEALSDSDKKILQKEVKRLAAALRTRIALRQKRAKSGQLDPKTTIRKNLKYQGV
                     PMEIHYRDKTRKPKIVIICDISTSMRFCSELMLSFLFALQGQVRKTHAFAFIDHLESI
                     SEDFNGADADQAISSVLWRMPSGHYNTDLGWSLDNFNKEHLDTLNGQTTLIIVGDGRN
                     NYNDPRLDIFSLMTRRAARTIWLNPEPPIMWHGDSDMPKYAPLCSNVLKVGNLRELVG
                     AVDSLLTV"
     gene            7841..8959
                     /locus_tag="VFQ23_26315"
     CDS             7841..8959
                     /locus_tag="VFQ23_26315"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET9910187.1"
                     /translation="MFGLSILVACTPAVSTEPVIPTNKSPIISTTTPEIESTATSPPT
                     ILPSVTTTLFAEQVKTRVPGTLTPTQVFPTITPFPKGQRIWFKGNPLPTHRLELSSAE
                     LNGRIYVAGGSHEKGLTYARMEVYDPLTDAWSTAAPMPISRHHFGMAALDGKIYVSGG
                     LPDWFRITDTCFVYDPATDSWSAIAPLPHRLATHSMASLDGKLYVVGGNNAPIAGGPM
                     EMFVYDAATDTWDDSLTPMPTGREHLAVVAAEGKLYVLGGRIQMGANLAIVEIYDPQS
                     DTWTRGPDMPVAVSGFTAAYLDGRIHATGGEDLFAMTVMNAHQVLDLATMTWEEWPEL
                     PGARHGVTSQAANGQWIVIGGSPRADMGMTGRVDIFVP"
     gene            8976..9752
                     /locus_tag="VFQ23_26320"
     CDS             8976..9752
                     /locus_tag="VFQ23_26320"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9910188.1"
                     /translation="MNETILTHQYDTILEITLNRPEAYNALNLDVMLMLNDSLASAAT
                     DDSIKGILITGKGKAFCAGGDLKWISQQAAGADSILHKLAPQFHIAITEIRRMGKPVL
                     AAINGIAAGGGFSLALACDFRVMAESAVLRQAYTSSGLSIDGGGTFSLPRLVGLARAL
                     EIAAFDQPISSAQALAWGLVTKVVPDDAVIEETMSMLQGLATSALHSFAWSKRLLTDS
                     FNNSLETQLELERQGISDCARHPNGQEGIRAFVEKRKPIF"
     gene            9861..10271
                     /locus_tag="VFQ23_26325"
     CDS             9861..10271
                     /locus_tag="VFQ23_26325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9910189.1"
                     /translation="MPNNPIFSYSFTVPENVIDDNGHVNNVAYVQWMQEAAIRHGESI
                     SEYQIAENATWYAREHRIEYLLPAFLGEKIEVRTWLTEIKRVRVHRKYEFTRKADGKV
                     IVRGETDWVHVDSKTGRPLAIPAIISELFPISPE"
     gene            10412..10777
                     /locus_tag="VFQ23_26330"
     CDS             10412..10777
                     /locus_tag="VFQ23_26330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9910190.1"
                     /translation="MPKILIVDDDVTITELMKALVRMDGHLPTTVNDSTQAIETANSV
                     NPDLITLDLMMPGLTGFELCELLHQDPKFANIPILIVSARDDSESKDRALKVGAKDYL
                     TKPFGVDNFLGKIKELTSS"
     gene            10795..11205
                     /locus_tag="VFQ23_26335"
     CDS             10795..11205
                     /locus_tag="VFQ23_26335"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9910191.1"
                     /translation="MPISSTYSKTIAIPESAIDENGHVNNVAYVQWMQDIAIEHYQFI
                     SGMNSMEPFGATWVVREHRIEYFLPAFAGEEIVIKTWVENIRRVRSLRQYEFVRKTDG
                     RILVKGETDWVFVDIKTGTPRAIPDEVSSVLKHT"
BASE COUNT         2985 a         2792 c         2758 g         2683 t
ORIGIN      
        1 ctcatcgcaa tacccacgtc cgcatttgac gcaagtccca agcgacggat tgtcacaacg
       61 atgcggcacc agataaccaa tgatcatttc acattgttga gccgttgtat ctgccatgaa
      121 gatcgcactc cttactgaaa agtatacacc agacatcggc gggcttgcaa tctcgacgga
      181 acgatttgcg cgtttattgt catccgccgc gcatgaagtg cacgtcttcg ctgcgacctc
      241 caacctgtcc ccttccgaaa aacggactct ctctcacgac cagatccatg tgacgcgttt
      301 cggcgcgcac aaacgtgtgg acgatactct ggtcgattgg tttgaacttg tcatcgagga
      361 gcatcatcga aacccgtttg atgtattgca cgcctatttt ctgacacagg caggctttat
      421 tgccacgtat gcaggaagat atctaaatat acccagcgtc gtgtctatcc gcggaaatga
      481 tatcgagcga gccgcgtttg atccgtccaa gttttcacat gtcatgttcg cgttgcaaaa
      541 cgcaagcgcc gtaaccgcca atgcaaaaga attggtagat cgaaccaggg cattcgtgga
      601 tcgtgaaatt gtcttgattc caaacggcat agataccgat catttcaaac caagtgagaa
      661 aataccaaag ttggttcaga cgttgcgttt gattgaagca agcagcttgc cattctcttc
      721 ctggactaca caaacttcac ccttccacca tcgtgctatt ggcttcgccg gtgaattgcg
      781 tgagaaaaaa ggactcaaga ctctcctggc tgcctacgcg caagtttgta agaaacattc
      841 atcaacatcc ctgctaattg tcggtgttgt gcgaccaggc ggagacaagg aatattttga
      901 cgattttcgc acacgaaacc ccaacttgca aatcatcgtc accggccata tatcgcctaa
      961 aggcctgccc aactactatg cactgatgga tgtatttgtc catccgtcgc ttcgagatgg
     1021 aatgcccaac gccctgctgg aggcgatggc ctgtggcaag ccggtcgtca cgactccggt
     1081 tggcggcatg ctggatgtaa tcaaagatgg tgaaaacgga atatttgtcc cggtcaatga
     1141 cagtgaggcg ctgtccaacg ccatcgaaga actgttgaat aatgctgagc gtcgaaatca
     1201 gctgggtacc gcagcccgtc aaacaatcct ggatggattt tctccgcaaa aggagcttga
     1261 ggcaaatctc gcggtgtatc ggaaactagg gctgaatcca tagccgagtc tttcagatat
     1321 aatggctcta agtatccatt caatgataca gggaatccat gtctgatctc agtggtaaaa
     1381 cactcggaca atatcagatc atcaagccaa tcggccaggg cggaatggcg acaatttatc
     1441 ttgcacagca accttcggtc aatcgagaag tagcaatcaa ggtcctggcc gctcctttac
     1501 aggaaaatgc cagttttgta aaacgcttca atcaggaagt tgaggtgatc gcacatcttc
     1561 aacatccaca gatcatcccc gtctatgact tcggcgaccg cggtgacgaa atttacatcg
     1621 tgatggcgta tatgcgaggt gggacactgg cagaacgcat cgagaaagca ccgaaaggtt
     1681 tacctgacgg ggaaacaatt cgtttgctcg acctgattgc agatggactg gatttcgccc
     1741 acaacaagaa catcgtgcat cgcgatctca agtccaataa catccttatg gatgaagacg
     1801 atcatccata catcgcagat tttggcctgg ccaagcttac cgagggaaag atcgaactga
     1861 caaataccat gatgaccggc acagcggcgt acatggctcc cgagatcgcg caaagtggca
     1921 aatcgacaaa acgagcagat atttacgccc tcggaataat ggtctttgaa atgctaaccg
     1981 gacgattgcc gtttgaggga gaaacgccat ataagatgtt gtcggcccat attcatgacc
     2041 ccgttccgaa tgtacgcaag tttcgccgcg acgtaccgcc agaagttcag ccagtcctcg
     2101 aacgagcgct ggccaagaat ccggtcaatc gctttgctac cgcaccagag tttgccgccg
     2161 cgctgaagga tgcattgaaa accaccagcc cggaggcggg ataccagtgg ctgctcaaac
     2221 cgcgagaatt tttccgtcga tcaccacggt tattgctggt ttctgtaact gccattctgc
     2281 tggctgggag tatattcttc acttcccgtc tttcaaacaa cgccgagctc ccgagcgcag
     2341 ttgttgatcc cacaaaacac atcgcccggg cgataaccga tgcaagcggc gtcacaacgt
     2401 ggacgtttgg atgtcttggc aatagcaaca gcgcactggt ggatctcgac tgccgagaaa
     2461 taagagttgc cgttgaaaat cgtattctcc cgtataacta cattttcctg gaaacgaatc
     2521 aacccggcgg catggattac gacatgtggt gggaaatctg tactcttctt cattgtcagc
     2581 ctgttttcat cgaggagaag tgggagactc tactggatga agtcagcaaa ggaaactatg
     2641 actcagcctc tgaaggcatc actattactc aagcacgccg agagatactc gacttttcga
     2701 tcagttacct gaacatagag caaagattgg ttgtgcgcaa aggcgagtcc cgattttcaa
     2761 atatcaacga gtttcttgca aacgagaact taattgcagg tgcattggag gattcaacaa
     2821 actttgatgc ggcaagagaa tatattccgg aagaccgtgt aaagacttat aaggaatttt
     2881 ccacggtatt ttatgcactg gctaccggtg atattgatgc ggccatcgca gatcaggccc
     2941 cgggtcaaac tacagtgaca ggcattgaat tgcagcaggc catcaacctg gagtttatag
     3001 gcacatcact ttcgagtgat cagttcggcg ttgtgttccc aaaaggaagt gatcttgtcg
     3061 atccaatcaa tgaagcgctg cagaatttac gcgcgcaggg gattctggat gaactaatca
     3121 aacgctactt tggcccgggc tttaccgtta cctataatga catcgatctg gggactcatg
     3181 gacaataaca ggctgatatc aattaatctc cattaatgtg ccgtttttca aagaggggta
     3241 tacttttctt tatttacagg tgataaatgt acaatccctt tgaatctaca aacacagtga
     3301 aatcgtcttt gaacggacaa aaatatatcg cgtcaaacga gatcgcaacc atcgtatatc
     3361 tcgcgcagaa gcttgggaag ccactcctca ccgagggacc cgcaggcgtt ggcaagaccg
     3421 aactggcaaa agcaattgca gcagctacgg gacgagacct gatccgcttg caatgttatg
     3481 aagggttgga tgaatcgaaa gccttatatg aatgggaata ctcgaaacaa ttgctttata
     3541 cacaactcct gcgcgacaag ttgaatgaca cgcttggcaa ggccgagtcc ctggctgaag
     3601 ccgcggacaa acttgccaaa gaagaagatg tgttcttctc caaccggttc ctgctgcagc
     3661 gtccgctatt gaaagccatc atgagtgacc agccatctgt cttacttatc gacgagatcg
     3721 accgcgccga tgcagagttc gaagccttct tactggaagt gttgagtgat tttcaagtct
     3781 cggtcccgga gctgggaacg ttgaccgcga aacataggcc cttcgtggtg ctgacatcga
     3841 acaacacgcg tgagttatcc gaagcattga aacgacgctg tttgtatctc ttcatcgatt
     3901 acccaacact tcaggaagaa ctcgcagttg tgcgattgaa ggtcccggat ttgaatccga
     3961 agctcgcaca gcaggccgtc gagtttgttc aaaagctgcg caaaggtgat atgcgcaaat
     4021 cgccatccat cagcgaaaca ttggattggg cgaatgcact cgtcgcactg aacgccaaga
     4081 gcctggataa agacacattg gaagatacgg tttcgatcct gctcaagcat gaggctgatc
     4141 tgcaaaaagc aaaacggcaa tatatcaatc ccccaccgcc caggcagaga ccagaggatt
     4201 tcggtaaatt ttctggaaac taagcaccaa cgctatgctc aaccctgaat ttgcatcaag
     4261 ctccactttt ggaattttgt ttttaatatt tgtgctgagt aacatggtct tgatgcattt
     4321 gatatttgta acatacgact cccttttgaa attagaatac aatttttatc gaatgtcttg
     4381 ggaaagcgat gggcaacctt ttggcttttt tgggattgac gctaaagacc aaagcttaca
     4441 aatttcgcta cctgccttcc tgaggaagca atatgatcag gctgtgcttt ctattaagtg
     4501 gcttttctca acaccttcgt ggatggtaaa tgacatacag gcattacaac ttgtaagcaa
     4561 gtatcgttgg ttaatgctga tttggataat ctttggtgcc atcccaacgt ttgtactcat
     4621 aatcaccgta ttttagatga aagaaactat cagcagcaac aaacatggat aacccacaca
     4681 acttcaaacg ccccacatcc gtcaaggaaa tgcaggagcg cttcgagaat tttgcaaccg
     4741 atgaaggttg gcagcatggt ttgaactata aaccagatcc cactgatatc ttcattgtga
     4801 ctccggcaaa atgcggaacg acctggatgc aacagattgt ccatggttta cgcacgcgcg
     4861 gttcgatgga ctttgacgag atcagccgcg tcgtgccgtg gatcaacatg gcatacgacg
     4921 tggggatcga tatttacgca ccgcaggtcg cgcgtccgca tgctttcaag acccatactc
     4981 gattggacga agtgccaaaa ggtggtaagt atattgtcat tgtgcgcgac ccgggtgacg
     5041 ctcttgtttc ggcatatcat tttatggaag aaatgttttt cgaaaaaggc acaatcacac
     5101 tcgaagaatt tgcacatgaa gaattccttc ccaatcgcga gatccacaca catacgctcg
     5161 ccttctggga ccgtcggaac gatgacgatg tcctgccgct cgcctatgaa aacatgaagt
     5221 caaacctcac tcgtaccatt gaacaggttg cggctttcat ggatatccca ttggatgaag
     5281 aactaaggga aattgtgttg aggcagtccg atatcaaatt tatgcaggaa cacaaggaac
     5341 agtttgaaga ccatctcatc cgcaaggcac gtagtgaagc gatgcgattg ccccaagatg
     5401 gtcaattaaa caaagtccga aatgggcaag tgggcgaatc aagagaacga gtaccggagg
     5461 aaatcataaa ggaactcgat gtcctgtggc aggaagttgt caccccgaaa actggtttgc
     5521 attcgtatga ggatctgcga aaggaactgg atcgttgact gtacgcaatg agttgactaa
     5581 atctgagtct cttctcttca gcgttgagga gcgcaatcgg attcgagacc gcgtgctcga
     5641 aatagcctcg tcggacgggc gcatcgtctc tggcgcggtt gtcggttcaa tggcgctgag
     5701 tgaaggagat cgcttctcag atctcgacct gacgtttggt gtcgtcgatg agttttcgat
     5761 tgacaatgtt cttgaggatt ggacgcgcca aatcatcgag gagttcgacg cggctcagct
     5821 cttcgatgta gccagcggcg cctctattta tcgtgtgttc ctgctgccgg gctgcctgca
     5881 atttgatctg tcttttacgc ctgcatctaa atttggcgcg attggaccga aatttaaatt
     5941 gctctttggc gatgccgtgg aaaagcctta ccttccaact caatctgcag cggagctttt
     6001 tggatatgca gttcatcatg ctttacgtgc ccgcttttgt attgagcgcg gtcgctattg
     6061 gcaggcagaa tattggatca gcagccttcg cgactatgcg ctttcgattg cctgtcaccg
     6121 acacagttta cctgtatctt atgccagagg cttcgaccat ctgcccgcag aggtgcttga
     6181 gccgttcaaa agtgggctag tagcatccct cgaacgcctt gaattgcttc gagcgctcaa
     6241 agttgtgatt gatggattaa tacgtgaatc agttgaagtt caggaaatag gaaacaaagt
     6301 cgagccacaa ctacgactac taacacgtga atggaacatt tagtgtaaga ggaacatttt
     6361 atggaatctc gaatcctaca gttaatttca gcattacgcg ccagcggtgt gcgcgtgtct
     6421 ttggcagaat cagcagaagc gttctctgca gttgacctga tggggattca ggatcgcgag
     6481 cagttccgtt tgtcattgcg cgcgacactc ataaaagatg tgcgcgattt gcctgtcttc
     6541 gataagctat tcccattgtt ctttggcacc gggacgccac ccatgatggg cggcaaccca
     6601 acagatgata tgactcctga agaggcacag atgctcgccg acgcgctcag gcagttcgcg
     6661 cagaatctgc gtcagcggat ggaacgcctg atgaacggcg agccgctcac caagtccgaa
     6721 ttggaagagc tgggtcagat ggttgggctt gatcagacgg atgacctccg ctatcaaaat
     6781 tggatggcac agcgcatgat gcgcgcgctg gaatatcccg aagtgcgcga agcgatgcgc
     6841 gagctgatgg aacaactcga gcaaatgggt atgagcaaag aacgtgtcga tgacctgcgt
     6901 gaacagatcc gtcaaaacct gcagggcatg caggagcaaa ttaaccagtt cgccggtcaa
     6961 cgcattgccg agaacctgag tcaacaacca ccatccgacg gcgtggataa tttgatgaac
     7021 cgtccgttcg aagcgctgtc agattctgac aagaaaattc ttcagaaaga agtgaagcga
     7081 ctcgccgcgg cgcttcgcac acgaatcgca ttacggcaaa agcgcgccaa gagcggtcaa
     7141 ctcgacccaa aaacaacgat ccgaaaaaac ctcaagtatc agggcgtgcc catggagatc
     7201 cattatcgcg ataaaacacg caaaccgaag atcgtaatca tttgtgacat cagcacctcg
     7261 atgcgtttct gttccgagtt gatgctgagc tttttgttcg ctttgcaggg acaggtgcgc
     7321 aagactcatg cgtttgcgtt cattgatcat cttgaatcga tctcggagga cttcaacggc
     7381 gcagacgctg accaggcaat ttcctccgtt ttgtggcgta tgcccagcgg tcactacaac
     7441 accgaccttg gctggtcgct cgataacttc aacaaggaac acctggatac gctcaacggt
     7501 cagacgaccc tgatcatcgt cggtgatgga cgcaataact ataacgaccc gcgcctcgac
     7561 atcttctcgt tgatgacccg ccgcgcggca cggaccatct ggctgaaccc tgagccgccc
     7621 atcatgtggc atggcgacag cgacatgccc aagtacgcgc cgttatgcag taatgtattg
     7681 aaagtgggga acttaagaga actggtcggt gcagtggatt ctttgttaac tgtgtgattc
     7741 tttgtaagca gtttcgaaaa caggattctg aaaacctaat atagcctcat gacacggaga
     7801 attcattgtg ccaaataaaa gaatcctaca atcggcctgc atgtttggac tttcgatctt
     7861 agtcgcctgc acaccagccg tttcaactga gccagtaatt cctaccaaca agtcaccaat
     7921 catatcgaca actacccctg aaatcgaatc cactgctaca tctcccccaa cgatcctgcc
     7981 ttcagttaca accaccctct ttgccgaaca agtcaaaact cgcgtccctg gcacccttac
     8041 cccaactcaa gtatttccaa ccatcacgcc ttttccaaaa ggacaacgaa tctggtttaa
     8101 gggaaaccca ctacccacac atcggcttga gttaagctct gccgagttaa acggcaggat
     8161 ttacgttgca gggggctcgc atgaaaaagg gctcacctac gcccgaatgg aagtctacga
     8221 ccccttgacg gatgcgtggt ccaccgccgc gcccatgcct atcagcaggc atcattttgg
     8281 gatggctgcg ctggatggaa aaatttacgt ctcaggcgga ctacccgatt ggttccgtat
     8341 taccgacacc tgcttcgtct acgacccggc cactgactcc tggtctgcaa tcgcccctct
     8401 accccaccgt cttgctacac acagcatggc gtcacttgac ggaaagcttt atgtggttgg
     8461 aggcaataac gcgccgattg ccggaggtcc catggaaatg tttgtctatg atgctgctac
     8521 tgacacctgg gacgattcgc ttacgccaat gccaaccggc cgcgaacatc tggctgtggt
     8581 cgcagccgag ggcaagctat atgtgctggg gggtcgaatt caaatgggtg caaacctcgc
     8641 aatagtcgag atctacgatc cacaatcaga cacctggacg cgcggtcctg atatgcctgt
     8701 cgcagtcagc ggattcaccg cagcttatct cgatggacga attcatgcga ccggcggcga
     8761 ggatttgttt gcgatgacag tgatgaatgc gcatcaggtg ttggatcttg caacgatgac
     8821 ctgggaagaa tggccagagc taccgggcgc acggcatgga gtcacatcgc aagcagctaa
     8881 cggccagtgg attgtgattg gcggctcgcc ccgcgcagat atgggaatga cagggcgtgt
     8941 ggatattttt gtgccgtgac cattcaagga attatatgaa cgaaactatt ctcactcacc
     9001 aatacgatac gattctggaa atcactctca accgccccga agcctataac gctctaaacc
     9061 tcgatgtgat gttgatgcta aatgatagcc ttgcatccgc tgccaccgat gattcgatca
     9121 aaggcatcct gatcactggc aaaggcaaag ccttctgcgc gggtggtgat ttgaaatgga
     9181 tcagccagca agctgccgga gctgattcca tccttcataa acttgcccct caatttcata
     9241 tcgcgatcac agagattcga cggatgggga aacctgtcct tgctgcgatc aatggcatcg
     9301 cggcaggtgg aggcttttca ctggcgctgg cttgtgactt ccgtgtgatg gcagaatcgg
     9361 ctgtgttgcg tcaagcctac acatccagtg ggttgagcat tgatggcgga ggtaccttct
     9421 ccctgccgcg tctcgtcggg ctggcccgcg cgctcgagat cgctgcgttc gaccagccga
     9481 tttcttctgc acaggcatta gcctggggac tcgtaacaaa agtggttcca gatgatgcgg
     9541 tgattgagga gacgatgtcc atgcttcagg gactcgcaac gtcagcattg cattcatttg
     9601 cctggtcgaa gcggctactc actgattctt ttaacaacag tctggaaact caactggaac
     9661 tggaacgtca gggcatcagc gactgcgccc gtcatccgaa cggacaagag ggcatccggg
     9721 catttgttga aaaacgtaag cccattttct agaattcaac cgggattaca ttactcgagt
     9781 ctgcggttac gtgcgaattc aaaatccgcc actcattttt cgccagggcg aatcaaagaa
     9841 gggaaacaaa gttttgttct atgcctaaca accctatttt cagctacagt ttcacagtcc
     9901 ccgaaaacgt aattgatgac aacggacatg tcaacaatgt agcatacgtc caatggatgc
     9961 aggaagcggc cattcgccac ggagaatcga tcagtgaata tcagattgcg gagaacgcga
    10021 cctggtatgc acgtgagcat cgcatcgagt acctactacc cgcatttcta ggggagaaga
    10081 ttgaagtccg cacctggctg actgaaatca agcgagtccg agtccatcga aaatatgagt
    10141 tcacgcgcaa agcggatgga aaagtaatcg tcagaggcga aacagactgg gtacatgtcg
    10201 actcaaaaac tggcaggccg ctcgcgatcc cagctattat ctccgagctt tttccgatct
    10261 caccagaata agctcaaaat cgaatcacta tatgtggtat ggggtcttaa cagaactgga
    10321 aactcttccg ggcaaatcga atactgctgc ttacgagacg cggtaaactt gtgcggggac
    10381 ccatttttcc tttccatata ggtgataacc catgccaaaa attcttattg tggacgatga
    10441 tgtaacaatt actgaattaa tgaaagcgct ggtaaggatg gacggtcact tgcccaccac
    10501 agtcaacgat agcacacagg caattgaaac tgcgaattct gtcaaccctg acctgataac
    10561 actggacttg atgatgccag ggctaaccgg ttttgaactc tgtgaacttc tccatcagga
    10621 tccgaaattt gcaaatatcc ccatccttat tgtcagcgcg agagatgact cagaaagtaa
    10681 agacagggcc ctgaaggtcg gggccaaaga ctaccttact aagcccttcg gcgtggataa
    10741 tttcctgggg aaaataaaag agttgacaag ttcataacaa ttacagcagg gcttatgcca
    10801 atctcatcca cctattcaaa aacgattgcc attccggaaa gcgccatcga cgaaaacggg
    10861 catgtcaaca acgtagcata cgtccagtgg atgcaggaca tcgccataga gcattaccaa
    10921 ttcattagcg gcatgaattc catggagcct tttggcgcta cctgggttgt ccgtgaacac
    10981 aggattgaat attttctgcc ggcttttgca ggcgaagaga ttgtgatcaa gacctgggtt
    11041 gaaaatatcc gccgtgtgag gtcattacgt caatatgagt ttgtccgcaa aaccgatggc
    11101 aggatattgg ttaaaggtga aacagattgg gtatttgtag atattaaaac gggaacaccc
    11161 cgcgcaattc ctgacgaagt atccagtgta ttgaaacaca cgtagcagaa gggtccta
//
LOCUS       DASTJP010000903         6109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_828974, whole genome shotgun sequence.
ACCESSION   DASTJP010000903 DASTJP010000000
VERSION     DASTJP010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828974"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VFQ23_26340"
     CDS             complement(<1..1574)
                     /locus_tag="VFQ23_26340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9910192.1"
                     /translation="MNFLKPPVFPKNEEKTRVARTLYQILMLSWSLPVLGMATSFLDP
                     ANLIFVPVAIILSVAILGLMVLNRAGRVRLAGMIYIFFLISIFTYIDTLTGINTPRPS
                     LTLFTSIIILISGLLLGGNAPILAAFVLIFIQIILFALSYMGFLAGSIPLPTFLQDIL
                     AIGTGYLLIAVLFREATIGIQNAVAKLRDSQKTLEATNLKLSELTRNLEQRVNERTGE
                     LGLANAKNERRAAQFEAIAQVSRTISSTQDLNTLLPQIASVISRLFGFYHVGIFLIDS
                     KGEFAVLSAANSEGGQKMLARNHRLKVGETGIVGYVTSTGSPRVALDTGADIVFFNNP
                     DLPYTRSEIALPFKVGGQVIGALDVQSKEPNAFAEEDVSILSILSEQVGIAIQNSRQY
                     EETRRALSESEMLSRQFVQRGWEQFTKGRSLAGIRHTGARATLLYTKNGDNVQSSNES
                     NSERQPESLVLPVKLRDELIGTVYVRTPGNPQWDQDELDIVNAIIERAAVSLENARLL
                     AESQKRAAKERTISEIS"
     gene            complement(1598..3367)
                     /locus_tag="VFQ23_26345"
     CDS             complement(1598..3367)
                     /locus_tag="VFQ23_26345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9910193.1"
                     /translation="MSTPKISKPFDSPKYWYNPFMALISPIWNFFTEPHPSVQEVGEF
                     RRAQLLSSITLILIASFTWAVLSNPTSMTTFVALYLTTLLSYALSRSRYYRIGTYFFS
                     YGITSLAFFSLFFGTSSGFDSAITSIAHISLIIASILLSLRGFSFLVLISTIAAFTAR
                     AYSQTPIPDDDVFFRVSGVFMSIGVILIGANIFRARVERDRLREVQNINNELEDIKGR
                     LEQRVDERTSELAAANEQTSRRAAQLQTITELSETIAQLQDLIEIFPTVTTLISERFG
                     FYHVGIFLVDQDHTYAILQAANSEGGKRMLTRRHRLRLGTGVVGFAAQTGQPRIALDV
                     GADAVFFDNPDLPNTRSEVALPLKTRGQTIGILDVQSTEAGAFSTEDLQVLTALANQV
                     SIALENARLLTETRTALAQVQEVYNEFSKTEWSRTISNIQQPGFRYRTGRVEMLESAL
                     QNPEVLAAISNGEIVAEQPDGFEEKRSSVAVPVKLRGEVIGILHVEANDSSKEWQKDE
                     VGLIAAVAERAAVAMENARLFQDARRRAVKEQTISEATARISSALNIENILHTTAQEL
                     ERVLTGSEIVIQFESAEPEGIKQ"
     gene            complement(3371..4774)
                     /locus_tag="VFQ23_26350"
     CDS             complement(3371..4774)
                     /locus_tag="VFQ23_26350"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9910194.1"
                     /translation="MNQRSTIPQSEAQRYAIVGILFGLLFPVVATIIRLWEAGIPFNL
                     SSLAAIQANDPLLWIIDTAPLFLGLFAAFAGRRQDRLQNLNSELRSRENELRIVQATL
                     EQRVDQRTQELFAANQRTEKRARQLQHIAEIARTAISMRTLDQLMPLLAQMISEQFGF
                     YHAGIYLMDELNQYAMLRASNSGGGFRLVQRGHLLKVGEKNVVSHVAQHGEVYIASDT
                     EDELFLIEAELPEANSEIALPLKSGNQILGVVDIQSVEKNAFSQDDIETLSVLADQIT
                     IAIQNAIFYDRSQRALQEAEVTSIQASSNAWREYTETIPAKGYRYSGIRPEPLKESLL
                     SNTDTNALHHPIQLRGRTIGNLGLKASNPARKWTEDELAILAATAERVAIALEGARLL
                     EDAQKRAARESFLSEIAAKLGTSFKLDSILRDTVEELGQTLKGSTISFQLVDPLANSN
                     SALPNSNGSSANSEKAE"
     gene            complement(4794..>6109)
                     /locus_tag="VFQ23_26355"
     CDS             complement(4794..>6109)
                     /locus_tag="VFQ23_26355"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9910195.1"
                     /translation="GLLLGWRQAIGFTLLSIFAVFRLATPETFASAVEGTVLIVFGAI
                     FLYLIITSLQDAVRKSQSSTNELQATNAQLTALRDDLELRVQERTAALEKRALQLQTV
                     ASLARTIATLKDLDFLLPEITKLVSEQFGFYHIGIFLIEEAGEHAVLQASNSEGGKRM
                     INRRHQLRLDSTSIVGYVTSRGESRITQDVGYDTVYSKNADLPETRSELALPLQAGGH
                     TIGALDVQSTQINAFSQEDISVLTTLADQIAIAIENSRLFGESTRALSESQLTIEKYV
                     KQAWDSFTQQAKQSGFMFDGKQVIPLEKGTRREQVKAAAKTGRLSLERASDTIAVPIK
                     LRGQLIGVLDVRSKNGQRQWTRDEIAMLEAAAERAALALENARLVDSAQRRAARERII
                     GEISTRIGAANNFDAILQTAVEELGRKIGGAEVTLEIESEQKQAS"
BASE COUNT         1551 a         1457 c         1469 g         1632 t
ORIGIN      
        1 gaaatttcgc tgatcgtgcg ttccttggct gcacgttttt gactttctgc cagtaggcgg
       61 gcattttcta aggaaacagc ggcgcgttca atgattgcat tcacgatatc caactcatcc
      121 tggtcccatt gaggattgcc gggtgtacga acataaaccg tgcctattaa ttcatcacgt
      181 aacttcactg gtagaaccaa tgattcaggt tgtcgctcag aattggattc attggaggac
      241 tgaacattat ctccattctt ggtgtataag agcgttgctc ttgcaccagt atggcgaata
      301 cctgccaggc tgcgaccttt cgtgaattgt tcccagcctc gctgaacaaa ttgtcgtgag
      361 agcatctccg actctgacag ggctctgcgt gtttcttcat actgcctgga attttgaata
      421 gcaataccca cttgctctga taagatcgaa aggatactga catcctcttc tgcaaaggca
      481 ttgggttcct tactttgaac atccagcgca ccgataactt gtccacccac cttaaaggga
      541 agagcgattt cagagcgcgt atagggcaag tctggattgt tgaagaaaac gatatctgca
      601 ccggtatcaa gcgcaacacg aggggatccg gtggaggtaa cgtacccgac gatgccggtc
      661 tcgcctactt ttaatcgatg atttctggcg agcattttct gtccgccctc gctgttcgcg
      721 gcgcttaata ctgcaaattc accttttgag tcgatgagga agatgcccac atgatagaaa
      781 ccaaataacc gactaattac ggatgcgatt tggggtaaga gcgtgttcag atcctgagtc
      841 gagctgatgg tgcgagagac ttgtgcaatc gcttcgaatt gagcggcacg ccgttcgttc
      901 ttggcgtttg caagacccag ttcgcctgtg cgttcgttta ctcgctgttc gaggtttctg
      961 gttaactctg aaagcttgag atttgtagct tccaatgtct tttggctatc tcgaagtttt
     1021 gcaaccgcgt tctgtatgcc tattgtcgct tctctgaaga gcactgcaat taagaggtaa
     1081 ccggttccaa tggcaagtat gtcctgcagg aaggttggaa gtggaattga accagccaaa
     1141 aaccccatgt aacttaaggc aaacaaaata atctgtatga atatgagaac aaatgcggca
     1201 aggattggag cattcccacc caataatagc ccactgatta gaataattat cgacgtgaag
     1261 agtgttagtg aaggtcgtgg tgtattaatt cctgtaagtg tatcaatgta tgtaaaaatg
     1321 gaaattaaga agaagatata aatcatacct gccagacgca ctcttcccgc gcggttcaac
     1381 accatcaagc cgaggattgc tacagacagg ataatagcca ccggaacgaa aattagattt
     1441 gcagggtcta agaagctggt cgccattcct aaaacaggca aactccaact aagcatcaaa
     1501 atttgataaa gggttcgggc aacgcgtgtc ttctcctcat ttttagggaa aacaggcggc
     1561 ttaaggaagt tcaagaacaa gttccgcata tgggccttta ctgtttaatc ccttctggct
     1621 cggcactctc aaattggata actatttctg agccagtaag cacgcgctcc aattcctgtg
     1681 cagtcgtgtg cagaatattt tcaatattga gcgcagaact aattcgtgct gttgcctcgg
     1741 aaatcgtctg ttctttgacg gcgcgccggc gtgcgtcctg gaatagacgc gcgttttcca
     1801 ttgcgacagc agcgcgctca gctactgcag cgatcaagcc aacctcatct ttctgccatt
     1861 ctttggatga atcattcgct tccacgtgta atataccgat aacctcgccg cgcagcttga
     1921 cgggtacggc cacagaggaa cgtttttctt caaacccatc gggctgttct gccacgattt
     1981 cgccgttgct tattgctgcg agcacctccg ggttttgtag agcactctca agcatttcca
     2041 ctcggcccgt ccgataccga aagcccggct gctgaatgtt tgatatggtg cgactccatt
     2101 ccgtctttga aaattcgtta tagacttcct ggacttgtgc gagcgctgtg cgagtttctg
     2161 tcagcaggcg ggcattttca agggcgatcg agacctgatt ggcaagcgca gtcagaacct
     2221 gaagatcttc tgtggagaac gcgcccgctt ccgtgctttg aacgtccaaa ataccgattg
     2281 tctgaccgcg agtttttaaa ggtaaggcaa cttcagagcg tgtatttgga agatcaggat
     2341 tgtcgaagaa aaccgcatca gccccaacgt ccagagcgat acgaggctgt ccggtttgag
     2401 ctgcaaagcc cacaacgccg gtccctaatc tgagccgatg tctgcgcgtc agcatccgtt
     2461 ttccgccttc gctgttcgcg gcctgcagaa tggcataggt atgatcctga tcgacgagaa
     2521 atattccaac atggtaaaag ccaaaacgct cactaataag cgtggtgacg gttggaaaaa
     2581 tctcgatcaa atcctgtagt tgtgcaatcg tttcagaaag ttcagtaatc gtctgcagtt
     2641 gggcagctcg gcgcgatgtt tgttcgtttg ccgcggccag ttcagaggtc cgctcatcta
     2701 cacgttgttc aagcctgccc tttatgtctt cgagctcatt attgatgttt tgcacttccc
     2761 tcaaacgatc tctttcgacg cgagcgcgaa aaatattcgc tccaatcaag atgacgccta
     2821 tggacatgaa tacgcctgaa accctgaaga aaacatcatc gtccggaata ggcgtttgtg
     2881 aatatgctct cgcagtaaat gctgcaatgg tcgatatcag aaccaagaag gaaaagcctc
     2941 tgagtgagag caaaatactg gcaataatga gcgaaatatg cgctatgctt gtaatcgccg
     3001 agtcaaagcc acttgaagtt ccgaaaaaca aggaaaaaaa tgcgagggat gtaattccgt
     3061 aggagaagaa ataagtaccg atcctataat atcggctgcg actaagagca tatgacaaaa
     3121 gggtagttaa gtacaacgcc acaaatgtgg tcattgaggt tgggtttgaa aggactgccc
     3181 aggtaaacga agcaatcagg atcaaggtaa tggacgacag aagctgagca cgtcgaaatt
     3241 caccgacctc ctgcaccgat ggatgaggtt cagtaaagaa gttccagatg ggcgaaatca
     3301 acgccataaa ggggttgtac caatattttg gtgagtcaaa tggttttgag attttaggag
     3361 tagacatgct ttactctgct ttctcgctgt ttgcagaact tccgtttgaa tttggtaaag
     3421 ccgagtttga attagccaag gggtccacca attggaatga aatcgttgaa ccttttaagg
     3481 tctgaccaag ctcttcgacc gtgtcgcgaa ggattgagtc tagcttgaac gacgtcccca
     3541 gttttgctgc aatttcagac aggaaagact cccgcgcagc gcgcttttgt gcatcctcaa
     3601 gcaagcgggc accttccagt gcaatcgcga cacgttctgc tgtggctgca agaatggcga
     3661 gctcatcctc cgtccatttg cgagctggat tagacgcttt gagccccagg tttccaattg
     3721 tccgaccgcg caactgaatt ggatggtgta aagcgttagt atcagtgttg gaaagaagcg
     3781 actctttcaa cggctctggc cgaatcccgc tataccgata tccctttgcc ggtatcgttt
     3841 cggtgtactc tctccaggca ttgctggatg cctgaattga cgtcacttcc gcctcctgca
     3901 aggcacgctg tgaacgatca taaaaaattg cgttctgaat cgcgattgtg atctgatccg
     3961 cgagaactga taaagtctct atgtcatctt gcgaaaatgc gtttttttca acggactgaa
     4021 tgtcaactac gccgaggatt tgattacccg atttgagagg caaagcaatt tctgaatttg
     4081 cttccggaag ttcagcttct atcaaaaaaa gttcatcttc ggtatcggaa gcgatgtaga
     4141 cctcgccatg ttgagccaca tgacttacga catttttttc accgactttc aacaaatggc
     4201 ctcgctgaac taaacggaat ccgcctccac tattggaggc gcgaagcatt gcgtactgat
     4261 ttagttcgtc catcaaataa atcccagcat gatagaagcc aaattgctca ctgatcattt
     4321 gagcaagcaa aggcatgagt tgatcgaggg ttcgcatcga gatggcggtc cgggcgatct
     4381 ccgcaatatg ctgcaactgt cgtgcgcgct tttcggttct ttgatttgca gcaaacagtt
     4441 cctgtgtacg ctgatcgacg cgctgttcaa gcgtagcctg gacaatccgt aattcatttt
     4501 ctctcgaccg caattctgaa ttcaggttct gaagtcgatc ctgtcgacga ccagcaaatg
     4561 ccgcaaacaa accgaggaat agaggggctg tatcaatgat ccaaagcaat gggtcattgg
     4621 cttgtatcgc tgcaaggctg gataaattaa agggaatccc tgcctcccaa agcctgatta
     4681 tggtagccac aactgggaac aataaaccaa agagtatgcc gactattgca tatctttgcg
     4741 cttcgctttg aggaatggtg cttcgctggt tcacgtttct gttctcaagg tgtttaagac
     4801 gcctgttttt gttcactttc gatctcaagt gtgacttcgg cgccgccgat tttgcggccc
     4861 aactcttcaa cagcagtttg aagaatcgca tcaaagttat ttgccgcacc aattcgtgtc
     4921 gaaatttctc cgatgatccg ttcacgcgcg gctctgcgtt gcgcgctgtc aacaagacgg
     4981 gcattttcca gggcgagcgc agcacgttcg gctgccgcct caagcatggc aatttcatct
     5041 cgcgtccatt gacgctgacc gttctttgaa cgaacatcca ggaccccaat caactgaccg
     5101 cgtagtttga ttggaactgc aatcgtatca gatgcccttt ccaacgaaag ccgacctgtt
     5161 tttgctgcag ctttcacttg ttctcgtctc gttccttttt ccagaggaat aacttgtttt
     5221 ccatcaaaca tgaaacccga ttgtttggct tgctgtgtaa aactatccca ggcttgtttt
     5281 acatattttt ctatggtgag ttgagactca gataaggcgc gtgttgattc gccaaatagg
     5341 cgagaatttt caatggcgat cgcaatctgg tctgccaatg tggtcagcac acttatgtct
     5401 tcctgagaga aggcgttgat ttgcgtgctt tgtacatcga gtgcgccaat ggtgtgccca
     5461 ccagcttgta aaggtaacgc caactctgaa cgtgtttcag gtaagtccgc gttcttcgaa
     5521 taaacggtat catagccaac gtcctgcgtg atgcgcgatt cacctcgaga agttacatat
     5581 ccaacaatac ttgtcgagtc cagccgaagt tggtggcggc gatttatcat tcgctttccg
     5641 ccttcactgt tagaagcttg aagaactgca tgttctccgg cctcctcaat gagaaaaatt
     5701 ccaatatgat aaaaaccaaa ttgttcgctg acaagcttgg taatttccgg aagaagaaaa
     5761 tccaggtctt ttaatgtggc gatggtccgc gccaggcttg ccactgtttg aagctgtagc
     5821 gcccgttttt cgagagcagc cgtgcgttct tgaacgcgta attccaaatc atctcgaaga
     5881 gcggtcaatt gcgcgttggt cgcctgtagt tcgttcgtgc tggattgtga cttcctgact
     5941 gcgtcttgaa ggctggtgat aatcaaatac aggaaaatgg caccgaacac aatcagaacg
     6001 gttccttcaa cagcgcttgc aaacgtttct ggagttgcaa ggcgaaatac agcaaaaatg
     6061 cttagcaggg taaacccaat ggcttgtcgc caaccaagca acaatcctg
//
LOCUS       DASTJP010000904         6278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_829565, whole genome shotgun sequence.
ACCESSION   DASTJP010000904 DASTJP010000000
VERSION     DASTJP010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829565"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..836)
                     /locus_tag="VFQ23_26360"
     CDS             complement(129..836)
                     /locus_tag="VFQ23_26360"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HET9910196.1"
                     /translation="MELTVKIFRYNPEKDERGHYETYKIEADQNDRVLDVLEHIKGYQ
                     DGSLSFRRSCAHGVCGSDAMRINGKNYLACKLLVRDVGANITVEPLLGLKVKKDLIVD
                     MEPFFDNYRKMLPYFINDSPLPADGKERLQSPEQRARFDESTKCILCAACTTSCPSFW
                     ASDEYYGPAALVTAHRFIFDSRDEGAAERLQIVAETDGVARCHTVYNCTDACPRDIKI
                     TKAIGELKLATVTGKLE"
     gene            complement(893..2686)
                     /locus_tag="VFQ23_26365"
     CDS             complement(893..2686)
                     /locus_tag="VFQ23_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9910197.1"
                     /translation="MANVHQFDVVVVGAGGAGLMSGLYASRHVKTAVISKLYPTRSHT
                     GAAQGGISAALGNYEEDRPEWHMYDTVKGSDYLGDQDAIEFMCNEAIEAVLELEHMGL
                     PFDRTPEGRISQRPFGGHTNNVTGKPVRRAAHAADRTGHMILQTLYQQCIKNKVTFFD
                     EFQVLDFIIVNGKAAGVVAVQLATGELHTFHAKAVIVATGGHGRIFEVTSNAYAYTGD
                     AAAILLRHGLPLEDMEFFQFHPTGIYKLGILITEGARGEGAVLINGNGERFMPNYAPT
                     VKDLASRDVVSRAIYTEIRAGRGIDGKNYVYLDIRPETVNKFAALDGRTNPDGSPYKI
                     TGDTVLTKIPDIVDFCRVYLGVDPVTQMMPTQPTAHYTMGGIPTNKFGEVVVDSNNTP
                     FPGLYAAGECACVSVHGANRLGTNSLLDLVVFGKHAGLKAAEYAKGAAFEKMPGDAEA
                     GATSAFEALKNGDGKENAFTISTEMKQVMFDEVGIYRTGTVMESALDKILELKDRYKH
                     VRVSDTGKIFNTELLNAWELGNMLDVAELVTVCALNRTESRGGHARDDYPERNDDNWL
                     KHTLAWKTNGKVTIDYKPVVITKHQPKARVY"
     gene            complement(2786..3199)
                     /locus_tag="VFQ23_26370"
     CDS             complement(2786..3199)
                     /locus_tag="VFQ23_26370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910198.1"
                     /translation="MATRTLPLSKRGFTFETVMWAFTRVTVLAMYGLMLVGLVGALIM
                     SARTGASLSDILYWAFFPNLAGNPLGASIGVTIFAKLLVFVFILVVSGHGVHGVLEIL
                     DDYFPSPLARRWSRNFIITYAVVANLIAIYVIWTS"
     gene            complement(3200..3562)
                     /locus_tag="VFQ23_26375"
     CDS             complement(3200..3562)
                     /locus_tag="VFQ23_26375"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910199.1"
                     /translation="MSLDRNVSLSGALGYKGGNPYWSYIMHRIGGTALFIFFTLYILL
                     LAGVGSVNAILNNWLFQIFMFVFGLFHVINGLRITIFDLFPRLMEHYRPAIQVGWLIY
                     AFLGGYAIIVILMNAFGG"
     gene            3708..4211
                     /locus_tag="VFQ23_26380"
     CDS             3708..4211
                     /locus_tag="VFQ23_26380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910200.1"
                     /translation="MKRITLVVLIAIPMLLVTACGSSTTPATEMPATDVPATEVPATQ
                     PAATEPPASSGTGIQVDITLADNTIESPVTTFQVGVPYTFVITNAGQRGHNFNISTPV
                     SEVGSLEAALETALLVVAQSELGPGASVTVEYMFPADAAGKNLEFSCLIRMHYQDGML
                     LPITVTQ"
     gene            complement(4281..5705)
                     /gene="icd"
                     /locus_tag="VFQ23_26385"
     CDS             complement(4281..5705)
                     /gene="icd"
                     /locus_tag="VFQ23_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET9910201.1"
                     /translation="MAYQNVKVPEGGTKISIQNGKLNVPDNPIIPFVEGDGTGRDIWR
                     ASARVFDAAVEKAYGGKRKIHWMEVYAGEKSFRMFQSWLPDETTEAFKEFLVGIKGPL
                     TTPIGGGIRSLNVALRKLLDLYVCLRPVRYFSGVPSPVKHPEKVDMVIFRENTEDIYA
                     GIEFANGTEENKKFRELFKEAFPKEYTKIRFPETSGLGFKPVSVEGSERLIRAAIEYA
                     LENKRKSLNFVHKGNIMKFTEGAFKDWGYALAKREFRNDIVTERETWILGNKEKNPDL
                     SVESNAKSIDPGFDMMSPAQQKGIMDEVEEALKLWDTHGDGKWKSKILIKDSIADVTL
                     QFVLIRPQDYDVIATMNLNGDYLSDALAAQVGGIGIAPGANINYVTGHAIFEATHGTA
                     PKYADLDKVNPGSVILSGEMMLRYMGWGEAADLVIKGIEGAINAKTVTYDFARLMEGA
                     TEVKCSEFGNAIIEWMDKALEPAK"
     gene            5939..>6278
                     /locus_tag="VFQ23_26390"
     CDS             5939..>6278
                     /locus_tag="VFQ23_26390"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9910202.1"
                     /translation="MLKLPGLIDPHVHLREPGATHKEDWDTGTSAALAGGFTMVLARP
                     NTKPPIFDAETLDIALNAARAKARCDYAQYLGAGPDNAEFVASIADKAAGLKMYTDST
                     IGELRLDDMSL"
BASE COUNT         1540 a         1694 c         1457 g         1587 t
ORIGIN      
        1 gcatctgaaa agacacggct tgcataatct ccagtctcta cacacaatta ttatttacca
       61 tttactcagt ggtctcgata cacccttcgc tacaaacgct cgaggctact cgaccaccgc
      121 gctgctcctt attccaattt ccccgttacg gttgccagct tcaactcacc gattgctttc
      181 gtaattttga tatcgcgcgg gcaggcatct gtacagttat aaaccgtatg acagcgcgct
      241 actccatctg tttccgcgac gatttgcagg cgctctgcag caccttcgtc gcggctatcg
      301 aagatgaagc ggtgtgcggt caccagcgca gccggaccgt aatattcatc acttgcccag
      361 aacgatgggc aggacgtggt acacgccgca cacaaaatgc actttgtcga ttcatcgaag
      421 cgcgcccgct gttccggaga ttgcaaacgc tccttgccat ctgcaggtaa aggcgaatcg
      481 ttgatgaagt agggaagcat cttcctgtag ttatcgaaga agggttccat atccacgatc
      541 agatcttttt tcacttttag accaagcaat ggctcaaccg tgatatttgc accgacatcg
      601 cgcaccaaaa gtttacacgc cagataattc ttcccattga tgcgcatcgc gtcagagccg
      661 cataccccat gcgcgcacga acgtcgaaac gaaagcgagc catcctgata acctttgata
      721 tgctcaagca catccaggac gcggtcgttt tggtccgctt ctattttgta cgtttcatag
      781 tgaccacgct catccttctc agggttgtat cgaaagattt ttacggttaa ttccataacg
      841 actctacttt ccaaatccta aaggttttca aaacctttag gattttattg aattagtata
      901 cccttgcctt tggttgatgc tttgtgatca ctaccggctt gtagtcaatc gtgacctttc
      961 cgttcgtttt ccaggcaagc gtgtgcttta gccagttgtc gtcgttgcgc tcggggtagt
     1021 catcgcgcgc gtgcccgcca cgtgactcag tccggttcaa cgcgcagact gtcaccaatt
     1081 cggctacatc cagcatgttg ccgagttccc aggcattgag caactcggtg ttaaaaatct
     1141 tgccggtatc tgacacgcgc acatgtttat aacggtcttt tagttcaagg attttgtcca
     1201 acgcagattc catcacagtt ccagtgcgat agatcccgac ttcatcaaac atgacttgct
     1261 tcatctcagt ggagatggta aaagcgttct ccttgccatc cccattcttc aacgcttcaa
     1321 atgcggacgt cgcacctgct tccgcatcac ctggcatttt ctcaaatgca gcccccttgg
     1381 cgtactctgc cgctttgagt ccggcatgtt tgccaaagac aaccagatcg agcagagagt
     1441 tggtcccaag gcgatttgcg ccatgcacag agacgcaggc acattcacct gccgcataaa
     1501 gcccggggaa gggtgtgttg ttgctatcaa ccaccacttc accaaattta ttggttggaa
     1561 tgccacccat ggtgtagtga gcggtgggtt gggtaggcat catttgggta acggggtcca
     1621 ctcccaggta gacgcgacag aaatccacaa tatctggaat ctttgttaaa actgtgtcgc
     1681 ctgtaatttt ataaggtgac ccgtccgggt tggtacgtcc gtccagcgcc gcgaatttgt
     1741 tgacggtttc cggtcgaata tccagataga catagttttt tccgtcgatg ccgcgtcctg
     1801 cgcgtatctc ggtgtagatc gcacgcgaga ccacatctcg cgaggccagg tctttgaccg
     1861 tcggcgcgta attcggcatg aaacgttcgc cattgccatt gatcaaaacc gcaccctctc
     1921 cgcgcgcgcc ttctgtaatc agaataccaa gtttgtaaat ccctgtcggg tggaattgga
     1981 agaactccat gtcttccagt ggcagaccat gacgcaaaag gatggcagcc gcatcccccg
     2041 tataagcata ggcattggat gtgacttcga agatgcggcc atgtccgccg gtggcaacga
     2101 tgacggcttt cgcatggaag gtatgcaatt cgcctgtagc aagctgcact gccacaaccc
     2161 ccgcggcctt accattgacg atgatgaaat caagcacttg aaattcgtcg aagaaagtga
     2221 ctttgttttt gatgcactgt tgatagagtg tttgcagaat catgtgaccg gtgcggtccg
     2281 ccgcgtgggc cgcgcgccgg actggtttgc cagtcacatt gttggtgtgc ccaccaaacg
     2341 gtcgctgtga gatgcgtcct tcgggcgtgc gatcgaacgg aagccccata tgttccagct
     2401 caaggactgc ctcaatcgct tcattacaca tgaactcaat ggcatcctga tcgccaaggt
     2461 aatcagaacc tttgaccgtg tcatacatgt gccattcggg ccggtcttcc tcatagttgc
     2521 ccagtgccgc agagatacct ccctgcgccg cgccggtatg ggagcgcgtg ggatagagct
     2581 tgctgatgac cgccgtcttc acatgacgag aggcatacaa gccagacata agccccgcgc
     2641 cacctgcgcc aacgacgact acatcaaatt gatgaacatt tgccataaat catgctccta
     2701 aaccttttca ccgcgaagca cacgaagtcc gcaaagaaaa ctattaagga cttttgcgtt
     2761 ctttgcgctc ttagcggtta attttttacg aagtccaaat cacatagatc gcaattagat
     2821 ttgctactac agcatacgta ataataaaat tacgagacca gcgccgcgct aatgggctgg
     2881 ggaaataatc atccagaatt tccaatactc catgaacgcc atgccccgag acgaccagga
     2941 tgaaaacaaa caccaataat ttcgcaaaaa tcgtaacccc gattgaagcg cccaggggat
     3001 ttcctgcaag gttcggaaag aacgcccaat agaggatgtc ggacaaactt gcccccgtcc
     3061 gtgcgctcat gatcagcgcg ccgacgagtc cgactaacat gagtccgtac attgccagaa
     3121 cggtgacgcg cgtgaatgcc cacatgacag tttcaaacgt gaagccgcgt ttggaaagag
     3181 ggagagttcg ggttgccatt tatcctccaa acgcattcat cagaatgaca atgatggcgt
     3241 agccgcccag aaaggcgtat atcaaccagc cgacctgtat tgccgggcgg taatgttcca
     3301 tcagccgggg aaatagatca aaaatcgtaa ttcgcaagcc gttgatgaca tggaataaac
     3361 cgaaaacaaa catgaagatc tggaaaagcc agttattaag aatggcattc acagaaccca
     3421 cgcctgccaa taacaaaata tacaatgtga agaagatgaa taacgcagtc ccgccgatac
     3481 ggtgcatgat gtaagaccaa tatgggtttc cacctttata tcccaaagct ccggaaaggc
     3541 tgacgtttcg atccaggctc atgaatgcct ccttgaggtt tcacagatgg ccctattatc
     3601 tcattaaatc gcgcgcttgt cttgtatgag cgaatgatta gagtattgtg ttgggggtaa
     3661 agtcccacac tacaatttca tcaagtgaca aaaggagaac atagttaatg aaacgaatca
     3721 ccttagtagt tttgatcgcc attcccatgt tgttggttac agcctgcgga agctccacaa
     3781 cgccagcaac cgaaatgcct gcgacagatg tacctgcaac agaagttcca gctactcagc
     3841 ctgcagctac cgaaccaccg gcatcgtccg gaacgggaat acaggttgat atcacgctgg
     3901 cagataacac gattgagtca cctgtaacga cctttcaggt cggtgtgcca tatacgtttg
     3961 tgatcacaaa cgcgggtcag cgcgggcata acttcaatat cagtacgccg gtttctgaag
     4021 taggcagcct cgaagccgcc ctggagaccg ctttactggt ggtcgctcaa tccgagcttg
     4081 gaccaggcgc cagcgtaact gtggaatata tgttcccggc agacgccgca gggaagaacc
     4141 tggaattttc gtgtttaatc cgaatgcatt atcaggatgg gatgttgctg cctattactg
     4201 ttacgcaata atttctgata tatataggga atcaaaagtc gggacgactc gtcccgactt
     4261 ttatctttaa ttggcatttc ctactttgca ggctccagcg ccttatccat ccattcaatg
     4321 atggcgttac caaactcgga gcatttcacc tcggttgcac cctccatcag gcgtgcaaaa
     4381 tcatacgtca cggtctttgc attgatcgcg ccttcgatgc ccttgatgac taaatcggca
     4441 gcttcgcccc agcccatata acggagcatc atttcccctg agagaatgac cgaacccgga
     4501 ttcactttat caaggtctgc gtatttgggt gcggttccat gagtcgcttc gaagatcgca
     4561 tgacccgtca catagttgat attcgctccg ggtgcgatac caatgccacc cacctgtgca
     4621 gccaacgcgt ccgatagata gtcgccgttg agattcatcg tagcaattac atcataatcc
     4681 tgcgggcgga ttaagacgaa ttgcagggtc acatctgcaa tggaatcttt aatcaggatt
     4741 ttggacttcc atttgccatc accatgcgtg tcccatagct tcaaggcttc ttccacttca
     4801 tccataatgc ccttttgctg agcaggagac atcatgtcga aaccagggtc aatacttttt
     4861 gcattgcttt caacactcag atcaggattc ttttccttgt ttcccaaaat ccacgtctca
     4921 cgctcggtga cgatatcatt gcggaactca cgtttcgcga gcgcataacc ccaatcctta
     4981 aaggcacctt ccgtaaactt catgatattg cctttgtgca caaagtttaa tgatttgcgt
     5041 ttgttctcca gggcatattc gatggcagcg cggatcaatc gctctgaccc ttcgactgaa
     5101 acaggcttga aaccaagtcc gctcgtctca gggaagcgga tttttgtata ttccttcggg
     5161 aaggcctctt tgaataactc cctgaacttc ttattctctt ccgtaccatt tgcaaattca
     5221 atgcccgcgt aaatatcttc cgtgttttcg cggaagatga ccatatccac tttttcagga
     5281 tgcttcaccg gagaaggtac gccgctaaag tatcgcacag ggcgcaggca gacatacaga
     5341 tccagcaatt tgcgaagtgc cacattcaat gaacggattc cacctccgat cggagtcgta
     5401 agtgggccct tgatgccaac cagaaattcc ttgaatgctt cagtggtttc atcaggcagc
     5461 cagctttgga acatcctgaa tgatttttcg cctgcataca cttccatcca gtggattttg
     5521 cgttttccgc cataggcttt ttccactgcg gcatcgaaca cacgcgccga ggcgcgccag
     5581 atatcacgtc ccgttccatc accttcgacg aacgggatga ttgggttatc gggaacattc
     5641 aacttcccgt tttgtatgga aatcttcgtg ccgccttcag ggactttcac gttctgatat
     5701 gccattgtat tctcctgagg aaaattcttt gcggattata tcatccgcgc tgctcttgac
     5761 agatttcatc atgtgcataa aatcatgccg agatgagaac ccgcatcttt cgagacttga
     5821 gactgcttcc gttacttcaa acatttcttc cccgttcatt agagagagcc actagcggct
     5881 ctcttttttt gccttcccct ctccctgagg gagaggggcc aggggtgagg tctgaatcat
     5941 gctaaaactc cctggcttaa ttgaccctca cgtccacttg cgcgaacccg gagcgactca
     6001 caaagaagac tgggataccg gcacctccgc cgcgctggca ggcggattca caatggtgct
     6061 cgcgaggccc aatacgaagc cgcccatctt cgatgccgag actctcgaca tcgcattgaa
     6121 cgccgccaga gctaaagcgc gctgtgacta tgcccaatat ctcggcgcgg gccccgataa
     6181 cgcagagttc gtcgcgtcca ttgcagacaa agctgcgggc ttgaagatgt acacagactc
     6241 aaccattggc gaacttcgcc tggatgacat gtccttgt
//
LOCUS       DASTJP010000905         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_724125, whole genome shotgun sequence.
ACCESSION   DASTJP010000905 DASTJP010000000
VERSION     DASTJP010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724125"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..801
                     /locus_tag="VFQ23_26395"
     CDS             10..801
                     /locus_tag="VFQ23_26395"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910203.1"
                     /translation="MEDVLNFARQKSVPFSSRGSAASSLVAHCLGITSPDPLRLNLYF
                     ERFLNPARKTPPDIDTDLVRRTTARMIVRNVSQVKKNTCEVIRLTEKGNLNGKIARRK
                     RDELGNRLPASSSRDLMIWVKLNCLKSSDKLETYPPLTQRLRSSPDSNILQRSRCEIL
                     LSGTMVNETYQITKRHPTSSANVIHDRTAGLPTGREPYGNGNSIVVGGLTSTQRRSGK
                     PTTHLHLRASAGGAKGVRNDCLFKENVGMRNAESRTNSTSSAKDG"
     gene            755..2563
                     /locus_tag="VFQ23_26400"
     CDS             755..2563
                     /locus_tag="VFQ23_26400"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HET9910204.1"
                     /translation="MQKAEQILQAVQKMGEKRTPLMRVYRSLFSEDLFLAAYDKIARN
                     RGALTVGTENDTADGMSLKRIQRIIEDLRYERFQFHPARRIQIPKKSGGTRPLGIPNF
                     TDKLVQEVLRMILEAYYEPRFRDSSHGFRPERGCHTALTKLRYEFKGAAWFIEGDIRG
                     CFDNIDHEVLMQILARDIRDGRLLNLLRMGLKAGYLEDWQYNRTYSGTPQGGTLSPLL
                     ANIYLHELDEYIEDTLIPQYTCGRKRAYNQEYDRLGERIKVARNADNKEMVKQLVSQY
                     RKLPSQNVNDPNYRRLHYVRYADDFVLSFVGPKVKAEDVKTAIGLFLKEKLHLEMSPS
                     KTLITHARTEHARFLGYDISVYHADHKVTPKGKQRIKTRSVNGCIRLGIPFGKIDELN
                     KDYMRGEKIIHDARLLAYSDAQIIDTYQRRFRGMAEYYKFATDRDKLAKLKYVMEVSL
                     TKTLAHKFKTTVARIYRRYAGKRIFQGYAYKVLTVEVPAKTKTMHIYWGAIPLNVAKP
                     GTDVLDDNTGLRNTILGSRTDLIQRLQANECEICSSQEHCEVHHIRKLSDLKTRWRGR
                     KEKPAWVTSMIALQRKTLVVCKRCHVTIHSGKPLPT"
BASE COUNT          840 a          712 c          690 g          629 t
ORIGIN      
        1 tttctcatcg tggaagatgt cttgaatttt gcaaggcaga aaagtgtgcc tttttcatcg
       61 cgcggatcag cagcttcgtc actggtcgcg cactgcttgg ggatcaccag tcctgatccg
      121 ctacggctca acctatattt cgaacgtttt ctcaaccctg cccgaaaaac acctcccgat
      181 attgacacgg atctcgtgcg ccgcacgacg gcacgaatga ttgtacgtaa cgtatcacag
      241 gttaagaaaa acacctgtga ggtcatccgg ttaaccgaaa agggaaacct gaacgggaaa
      301 atagcacgcc ggaaaaggga cgaactggga aaccgtttac cagcgtcgtc gtcccgagac
      361 ctgatgatat gggtaaagct aaactgcttg aagtccagtg ataagttgga gacgtatcct
      421 cccttgacac aacggttaag gtcaagcccc gacagcaaca tcctgcaaag atcgagatgt
      481 gaaattcttc tgtcgggaac gatggtcaat gagacctatc aaatcacgaa aagacatcct
      541 acgtcatctg ccaacgtcat tcatgaccgt actgcgggat tgcctacggg gcgcgagccc
      601 tatggcaacg gaaactccat agtagtcggt ggtctaacgt ccacccaacg gcggtcggga
      661 aaaccgacca cacacttgca cctgcgcgca agtgcaggtg gggcgaaggg agttaggaac
      721 gattgcttgt tcaaagaaaa tgtaggtatg cgtaatgcag aaagccgaac aaattctaca
      781 agcagtgcaa aagatgggtg aaaaacgtac ccctctgatg cgggtttatc gcagcctgtt
      841 cagtgaggac ttattcctcg ctgcctacga caaaatagcc cgtaatcgtg gcgctttgac
      901 tgtgggaaca gaaaacgaca cggcagatgg catgagccta aaacgtatcc aacgcatcat
      961 cgaagatttg cgttatgaac ggttccaatt ccatcccgcc cgccggattc aaatccccaa
     1021 gaaaagcgga gggacacgcc cgctcggtat tccaaatttc acagataagt tagtgcagga
     1081 agtactgcga atgatattgg aagcctatta tgaacctcgt tttcgagaca gttcacacgg
     1141 ctttcgacct gaacgcggtt gtcatacggc actgaccaaa ctgcgatatg aattcaaagg
     1201 cgcggcgtgg ttcatcgaag gcgacattcg cggatgtttt gacaacatcg accatgaagt
     1261 cctgatgcaa attcttgcaa gagatattcg ggatggtcga ttgctcaatc ttttgcgaat
     1321 gggactaaaa gcaggttatt tggaggactg gcaatataac cgaacataca gcggaactcc
     1381 acaaggcggc accctcagtc ctctgctggc aaacatctac ctgcatgaac tggatgagta
     1441 catcgaagat acgctaatcc cgcaatacac atgcggaaga aagagagcct acaatcagga
     1501 atacgacaga ttgggagaac gcatcaaagt tgcgcggaac gcagacaata aagaaatggt
     1561 caagcagctt gtgagccaat accgcaaact cccatctcaa aatgtgaatg acccaaacta
     1621 ccggcggttg cattatgtcc gttatgcgga tgacttcgtc ctcagttttg tcggaccaaa
     1681 agtaaaagcg gaagatgtga aaacagccat cggtctgttc ctgaaagaaa aactccacct
     1741 tgaaatgagc ccatccaaaa ccctcattac acatgcacgc accgaacacg cgcgatttct
     1801 tgggtacgac atcagcgttt accacgcaga ccacaaagtt acaccgaaag gcaaacaacg
     1861 cataaaaact cgaagtgtca acggctgtat caggttgggt atcccctttg gcaaaattga
     1921 tgaactgaac aaggattaca tgcgtggcga aaagatcatc catgatgcca gattgctggc
     1981 atactcagat gcgcaaatca tcgatacgta ccagcgcaga tttcgtggca tggcagaata
     2041 ttacaagttc gccaccgaca gagacaagct cgccaaactc aagtacgtga tggaagtttc
     2101 actgacaaag acactggcgc acaaattcaa gaccaccgtt gcccgcatct acagaagata
     2161 tgcagggaag cgtatctttc aaggatatgc ctataaagtt ctcactgtgg aagtccctgc
     2221 taaaaccaaa acgatgcata tctactgggg tgctattccc ttgaatgttg ctaaaccggg
     2281 cactgatgta cttgatgata ataccggact gcgaaacact atactcggtt cacgtactga
     2341 cctgatccaa aggttgcaag ccaatgaatg tgaaatttgc agttcacagg aacactgcga
     2401 agttcatcac attcggaaac tctcagactt gaaaacccgt tggcgtggac ggaaagagaa
     2461 acctgcctgg gtaacatcga tgattgcatt acaaaggaaa acgctggtag tgtgcaaaag
     2521 atgccacgta acaattcatt caggaaaacc gctacccacc taaagcactt tcgttctgga
     2581 gagccggatg atgcgaaagt atcacgtccg gttcggaggg gggactgtgg aaaagtgtcc
     2641 gcaaggataa ctcgccactt tcctacccta ctgctcgcgc cgccgcgacc aagttattcg
     2701 ccatgtcttt gatacttatg gtgcagaccg agttgctgtc gtagccacta ttaatcgtta
     2761 tcgccccaag tctgctctag gcgatattgc caaggcacac ggtttggaat cagccaaagt
     2821 tcgagagctg tccaaccagc tcccgcatgc tttttggatg cgatttgcca c
//
LOCUS       DASTJP010000906         6045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1253302, whole genome shotgun sequence.
ACCESSION   DASTJP010000906 DASTJP010000000
VERSION     DASTJP010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253302"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFQ23_26405"
     CDS             <1..556
                     /locus_tag="VFQ23_26405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9910205.1"
                     /translation="YNLGRAMRLAEEIGPVECRHFTGSEMNEGVIQTIIEINRYSHFP
                     RLYNLPIEQIFLKTLAEQMKHSDWFGAFILYVNRQPVAYEYGFRFNGRFEDWRAGFDT
                     RLSSTISIGKALASMVIQACYAQATAEIDFLRGDEPYKLEWKPLAREYANTRVFNPRA
                     PGAIAAYQWLVNIKPRLKKFSSRK"
     gene            561..1778
                     /locus_tag="VFQ23_26410"
     CDS             561..1778
                     /locus_tag="VFQ23_26410"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HET9910206.1"
                     /translation="MKKKESRTVHPIVYVALILLTINAIFLGMLYFQWDRPGVQITNT
                     PVASAPSQESSLETSTAGAPTNATARLAWFTSLPKNSEDIFKIAEWFDLFILIQGDED
                     SRDTIIDLGVDGPILQYVNFDSIQDPGSCTESPKINQVADLPGDFCQISEQHPDWFLL
                     DFNGQRIALQDGDNNWYLMDPGNEGWQAFFLERVKGFQADPNWDGIFLDNVPVTLSFR
                     EADGNLPAAYPNDASYQAAVQSFLKFIYEGYFQPNRKLLFANLVSRKDDADWVNHIQY
                     LDGAMHEGWAIDWPDGYRSAEAWEKHMRLAEQTQQLGKYIVLVSQGTQEDTTLQEFAF
                     ASYMLINHGRAAFRYANSDDYNEIWLYDNYANSLGDALGDRYPVGAAWKRDFANGSVS
                     VNPETHEVEILIK"
     gene            complement(1853..4186)
                     /locus_tag="VFQ23_26415"
     CDS             complement(1853..4186)
                     /locus_tag="VFQ23_26415"
                     /inference="COORDINATES: protein motif:HMM:NF015149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HET9910207.1"
                     /translation="MKSRISFFLFVSLVVIGSLLLNLSSPVSAQGTDATPTSQTPLPE
                     GTSIASTPTTSSLEETLSTGANTINFFQIRQEDIQLVGPYDVAFLVFGLPAEWELNAA
                     ATLNLSLHVSVSAVSATLGNELVSGAGGTLSVEFNRQVVGSFPLPQNEDLTIPVQIPL
                     ELLEPVRDDGHQEIVFVLDSGFSCLINQQMSLTIRSSSNVTFPHAIVLPDTNLARFPF
                     PIYQDTIYPDSALMVIPDQPTAEELQSAMALSAGLGRMSSSSILLDLTTVSKISPEQI
                     ASNNIILVGKAASFPLLDQLQLPLAPAAGRFQSEADDGIVQLVHSPWGTGRVVLLVSG
                     NSDAGVVKATQAVSTGILRQNATPNLSVIESVQESIQQLQQNTDFTLMDQGYESAVLQ
                     RRGVDSVSYNFYIPPGMTVSPDAYFELVYGNSALIDYARSGLVVQANGQPIGSVRFSD
                     ETASNTTNRSQINIPASVVLPGTNTVEIISNLQPTDNCSIPNLRGLWANIWSESRFHL
                     PLIQSVAETNLAIDLADFPAPFVLDPSLRTTAFVLQPNDLESWRSAQQLALYLGDRGN
                     GSIVLLKSFYADAIPESARTDLNLIVIGLAPQMPIMTELNQFLPAPFESEAGIASERN
                     MQVTFRIPADSPVGYVEFMPSPWNAKNYVVVAVGNLRQGANWAASGLYDASLRSRLAG
                     NFAVINDQQVTTTDTRISSSFAENPIATTQSEVVVVPPSVDTTVPVPVSRPIWILPVL
                     MVTVGLIALILIGVVYNSAVQSRRRGKFKPAAEEEKS"
     gene            complement(4233..5309)
                     /locus_tag="VFQ23_26420"
     CDS             complement(4233..5309)
                     /locus_tag="VFQ23_26420"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9910208.1"
                     /translation="MIETANRDIVDGLAKALIAKIPEWYGVDASLDLKAIALHTYPYS
                     FFVSFPVQTPSGKQTLFAKIHRKPHMETLKEALAADWLRPIAHAEYEITKVIWQTFES
                     KNSPVCTAIQPLGLADEWNAILMRKVEGKELKKFLVRPSVLFRSGPALMELQKYLEAS
                     MIWLRIFHDHVSEAQMAPFPVDDALESMDEILEKLKQCSNAEVDVEFYRNELLNRLES
                     IRDLTVPVALLHDDFQYSNILIQNDGRACVLDYAFNHRGCVYSDLATFLIDPQTRGVQ
                     ILTRGIFIAPAFLHAFRTTALTAYFNGEGYHKDVLDFYCLLAILNKWSADEGAIYSGW
                     KRRLYSFFVPSMRHYYTNLLTEYF"
     gene            complement(5302..>6045)
                     /locus_tag="VFQ23_26425"
     CDS             complement(5302..>6045)
                     /locus_tag="VFQ23_26425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9910209.1"
                     /translation="NFASRFTTRIITVAEGERELAQKNGVGRNDQFTVIHTGIDPTPY
                     RQPIDKIQLKTMMGIPASSPVIGSVGRLSEQKSPFDFVRVAERVHRSRPDAHFVWVGD
                     GPLESEVRTLSSSLNLDSVIHWLGQRKDVPQLLHTFDFFLLTSRWEGFPLVILEAMAS
                     SVPVVATNIPGTREAIQHGVNGLLANVGDFETMARFILDLLAHPVKADSFRSASRLRI
                     DTEFTRDRMLSMIEELYLEVASQGRAAHD"
BASE COUNT         1646 a         1455 c         1445 g         1499 t
ORIGIN      
        1 ctacaacctt gggcgcgcca tgcgcctggc ggaagagatt ggtccggtcg aatgcaggca
       61 tttcacggga agtgagatga atgagggcgt gatccaaacg attatcgaaa tcaaccgtta
      121 ttctcatttt ccacgcctgt ataacctgcc aatcgagcag attttcctga agactcttgc
      181 tgaacaaatg aagcactccg attggtttgg ggcatttatt ctctatgtca accgacagcc
      241 cgttgcgtat gagtacggat ttcgcttcaa cggccgtttt gaagattggc gggcaggctt
      301 tgacacccgg ctatcatcca ccatttcgat tggaaaggcg ctggcgagca tggtcatcca
      361 ggcgtgttac gcgcaggcga ccgcagagat cgattttctg cgaggtgatg agccgtataa
      421 acttgaatgg aaacctctgg ctcgtgaata tgcaaatacg cgggttttca atccgcgtgc
      481 cccaggagcc attgctgctt atcagtggct ggttaacatt aaacctcgcc tgaagaaatt
      541 tagctcacgg aaataattgt atgaagaaaa aagaaagtcg cacggtccat ccaatcgtat
      601 atgttgcatt gattcttttg acaattaatg caatttttct tggaatgtta tatttccaat
      661 gggaccgccc aggcgtacaa ataacaaaca caccggtcgc ttctgcccct tctcaggaat
      721 catcgcttga gacctcgact gctggcgccc caacgaatgc cacggctcgc cttgcgtggt
      781 ttacaagtct gccaaaaaat agtgaggaca ttttcaaaat tgctgaatgg tttgatctgt
      841 ttatccttat tcaaggtgat gaagacagtc gtgacactat cattgacctt ggggtcgatg
      901 ggccgatttt gcaatacgta aattttgact cgatacagga tcccggctcc tgcaccgaaa
      961 gccctaagat caatcaggtg gcggaccttc ccggtgattt ctgtcagatc agcgagcaac
     1021 acccggattg gttcttgctg gacttcaacg ggcagcggat tgctctccag gacggagata
     1081 ataactggta tctgatggat cctggtaacg agggttggca agctttcttc ctcgaacgag
     1141 tgaaagggtt tcaggctgat ccaaattggg atggaatttt tctggataat gtaccggtta
     1201 cgctctcctt cagagaagca gacggtaacc tccccgcagc gtaccccaac gatgcgagtt
     1261 accaggctgc agttcagtcg tttttgaaat tcatctacga gggatacttt cagccgaatc
     1321 gtaaactgct ctttgccaat ttggtctcta gaaaagatga tgcggattgg gttaatcata
     1381 tacagtatct tgatggagcc atgcatgaag gctgggcaat tgattggcca gatggctatc
     1441 gttcagcgga ggcatgggaa aaacacatgc gtctggctga acaaacacaa caactcggca
     1501 aatatattgt tctcgtctcc caaggaactc aggaagatac aacgctgcag gaattcgcat
     1561 ttgcttcgta tatgctcatc aaccacggcc gtgctgcgtt tcgttatgcg aacagcgatg
     1621 actacaacga gatatggctt tatgataatt atgccaacag cctcggcgac gcgttgggtg
     1681 atcgttatcc ggtgggcgcg gcctggaaga gggattttgc aaatggaagc gtgtccgtca
     1741 accctgaaac gcatgaagtg gaaattttga ttaaataaaa ggcatcggca gtcactctcc
     1801 gccgatgcct tactcagata catttgctgc tgaggtttaa taggggcaat acttatgatt
     1861 tttcctcttc cgcagcaggc ttgaatttgc ctcgtcgtcg gctttgaacc gcggagttat
     1921 aaaccacgcc aattagtatc aaagcgatca aaccaacagt caccatcagc accggtaaaa
     1981 tccaaatagg gcggctgaca ggcaccggaa ccgttgtatc cacagaaggc ggaacaacga
     2041 ccacttctga ctgtgtcgta gcgatcgggt tttcggcaaa agaggaggag atacgcgtgt
     2101 cggtcgtggt gacttgttgg tcattgatca cggcaaaatt gccagcaaga cgcgaccgca
     2161 aggaagcatc atacaaaccg gaagcagccc agttcgctcc ctggcgtaga tttcccaccg
     2221 cgactacaac atagtttttg gcgttccacg gagagggcat aaattctaca tagccaactg
     2281 gtgaatcagc cgggatgcgg aaagtcacct gcatattacg ttcgcttgca ataccggctt
     2341 ccgattcaaa gggtgccgga aggaattgat tcaactcggt catgatcggc atttgaggcg
     2401 ccagcccaat gacaattaga ttcagatccg tacgagcaga ttccgggatc gcatctgcat
     2461 aaaaagattt tagcaacacg atcgaaccat ttccacgatc ccccagatac aaggctagct
     2521 gctgagcgga tcgccaggat tccaaatcat tgggctgcaa aacaaaggct gttgtgcgca
     2581 acgagggatc cagtacgaac ggagctggaa agtcagctaa atcaatcgcc agatttgttt
     2641 cggcaacgct ctggatcaga ggaaggtgaa agcgcgattc tgaccaaata tttgcccaca
     2701 atccacgcaa gtttggtatg gaacaattgt ccgttggctg aaggttcgaa atgatctcaa
     2761 ccgtatttgt tcctggcaac accaccgagg ccgggatgtt aatttgactt cggttagtag
     2821 tgtttgaggc tgtttcatca ctgaaacgaa cacttccgat tggctgacca ttggcttgaa
     2881 cgaccaatcc tgaacgtgca tagtcaatca gcgcagaatt tccatagacc agctcaaaat
     2941 aggcgtctgg acttaccgtc attcccgggg gaatgtaaaa gttgtaagaa actgaatcaa
     3001 ccccccttct ctgaagcacg gcgctttcat agccctggtc cataagagta aaatctgtgt
     3061 tttgctggag ttgctggatg ctttcctgca cactctctat gactgatagg tttggcgtcg
     3121 cattctggcg caaaataccg gtggaaacag cctgtgtggc tttcacaaca ccggcatccg
     3181 aattccccga aaccaaaagt acgacacgcc ccgtgcccca tggcgaatgt accagttgta
     3241 caatcccatc gtctgcctca gattgaaagc ggcctgcagc cggcgcaagt ggtagctgca
     3301 attgatctag aagtgggaac gacgcagctt tccctactaa aatgatatta ttgctggcaa
     3361 tctgctccgg gcttattttg ctaacggtgg tcaggtccag caggattgat gaactggaca
     3421 ttctgcccaa ccctgcagac aaagccattg cgctttgcaa ttcctcggca gtgggttgat
     3481 cggggatcac catcagcgca ctatccggat aaattgtgtc ttgatagatc gggaacggaa
     3541 atctcgccag gttagtatct ggcaatacaa tcgcatgagg aaaggtcaca ttggaggagc
     3601 tacggatcgt caaagacatt tgctgattaa tcaaacacga aaatccacta tctaagacaa
     3661 acacgatctc ctgatgacca tcatctctta caggttcaag cagttccagc ggaatttgta
     3721 cgggaatggt cagatcttca ttttgcggca atggaaagga gccaacaacc tggcgattga
     3781 attcgaccga aagggtgcct cccgcacccg agaccagttc gtttcccagg gtagccgata
     3841 ctgcacttac agaaacatgc aggcttaaat ttagcgttgc tgctgcattc aattcccact
     3901 ctgcgggtaa gccaaatacc aagaatgcca catcataggg tccaaccagt tggatatctt
     3961 cctgtcgtat ttgaaaaaaa ttaatggtat tagcgcctgt cgaaagagtt tcttccaggg
     4021 aagaggtggt cggtgttgat gcaatacttg tgccttccgg caggggagtt tgcgatgtcg
     4081 gtgttgcatc cgtaccctga gcactgactg gggatgaaag attcaacaag aggctcccga
     4141 tcacgactag agagacaaag agaaaaaacg agattcgaga tttcatagaa gctcctaacg
     4201 tcaattctat actacaaaac cgaacaaagt catcaaaaat attctgtcaa caaatttgta
     4261 tagtaatggc gcatggatgg tacgaaaaag gaatacaatc tccttttcca gccgctatag
     4321 attgcaccct catctgcgct ccatttgtta agaatggcca gtaaacaata aaaatccagc
     4381 acatctttgt gatagccctc cccgttgaaa taggctgtca aagcagttgt ccgaaaggcg
     4441 tggagaaatg ctggcgcaat aaatatgcct cttgtgagta tttgtactcc acgcgtttgc
     4501 ggatcgatga gaaatgtagc aaggtctgaa taaacacagc cacgatgatt aaaggcgtaa
     4561 tccaaaacac aggcccgccc atcattttgg atcaagatat ttgaatattg aaaatcgtca
     4621 tgaagcagcg ccaccggtac ggtcaaatct cggatgcttt ccaggcgatt taataattca
     4681 ttccgataga actccacatc cacttccgca tttgaacatt gtttcaattt ttcaaggatc
     4741 tcatccatcg attccagagc gtcatcaaca ggaaagggag ccatctgagc ttccgatacg
     4801 tggtcatgga agatccttaa ccaaatcatg gaagcctcca gatatttttg caactccatc
     4861 aatgccggtc ccgatcgaaa taaaaccgac gggcgcacca aaaatttctt aagctcctta
     4921 ccctccactt tgcgcatcag gatggcattc cattcatctg ccaatccaag gggttgaatg
     4981 gccgtacaca ccggagagtt tttactctca aacgtttgcc agatcacttt ggttatttca
     5041 tattcagcat gcgcaatggg acgcagccaa tcggccgcca acgcttcctt cagggtttcc
     5101 atatgaggct tacgatggat cttggcaaaa agagtttgtt ttccgcttgg ggtttgaacc
     5161 ggaaaactga cgaagaagga atacggataa gtatgtaaag ctatcgcctt taggtccagg
     5221 ctggcatcca ctccatacca ttctgggatt ttagctatca atgcctttgc cagcccatca
     5281 actatatctc gatttgcggt ctcaatcatg tgcagcacgc ccttgtgaag ccacttcgag
     5341 ataaagctct tcaatcatcg atagcattcg atctcgcgta aattcagtat caatgcggag
     5401 gcggcttgca gagcggaagg aatcagcttt gacggggtga gccaacaaat ccaggatgaa
     5461 gcgagccata gtctcaaaat cgccaacgtt tgccagaagt ccattaacgc catgctgaat
     5521 tgcttcgcgc gtgccgggga tgtttgtggc aacaaccggg acgctggaag ccatagcctc
     5581 tagtatcaca agagggaaac cctcccaacg cgaagtaagc aggaagaaat caaaagtatg
     5641 aagtaattga ggaacatcct tacgctgacc aagccaatgg atgaccgaat ccaaattaag
     5701 agaggaactt aacgtccgga cttcggattc aagcgggcca tcgcccaccc aaacaaaatg
     5761 cgcatccggt ctggatctgt gaactctctc cgccacccgc acgaagtcga aaggtgattt
     5821 ttgttcactt aaccttccca ctgaacctat cactggcgat gaagcaggaa tgcccatcat
     5881 ggttttcaac tgtattttat caatcggctg acgatatggc gttgggtcaa ttcccgtatg
     5941 aatcacagta aactgatcat ttctaccaac gccatttttt tgggccaatt cacgttcgcc
     6001 ctctgcaaca gtaatgattc gcgtggtaaa gcgtgaagca aagtt
//
LOCUS       DASTJP010000907         8582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1360625, whole genome shotgun sequence.
ACCESSION   DASTJP010000907 DASTJP010000000
VERSION     DASTJP010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360625"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFQ23_26430"
     CDS             <1..1038
                     /locus_tag="VFQ23_26430"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET9910210.1"
                     /translation="GVRRLTQDGHHCLVERNAGLGAGFSDNDYLAAGAQIIYAGEEIY
                     GRGDMILKIARPTLEEISWMIPRQTLLGFLHLHSAQPNKIQLMLEKHITAISYEQIEY
                     QDGTRPVLKPLSQIGGQMAAQMAAQILQNNSGGNGVLLGGVPGVPPAEVVIIGVGVVG
                     ESAARAFLGMGAHVTLLDSDPDRLQAVAVSLPRHVISLASNPLNIERTCSLADVVVGA
                     VHMDHERAPVIITKEMIEKMRTGSVFLDLSVDEGGCAETSRPTTHDNPTYTVDGVIHC
                     CIPNLSGVVARTATLAFQNAAWHFIEQISRHGVRDVMSIDPALASGVSMDRGKLQNPE
                     QYLDIPTRGLR"
     gene            1035..2360
                     /locus_tag="VFQ23_26435"
     CDS             1035..2360
                     /locus_tag="VFQ23_26435"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET9910211.1"
                     /translation="MNWNDLYNQRVTSAEVAVKQINSGMRVFLSGNCSVPQTVLAALV
                     ARAHDLQDVEIIQVLSIGGADYVSPDMAGHLRINTLFISDNVREAVNDGRADFTPAHL
                     SEIPRLFSSGVLPIDVALIQVSPPDEHGYCSFGVEVGVTKTAAQYARIVIAEVNPQMP
                     RTLGDSFIHVSKIHQIVPVHYPLPEVSMGEPTEVSKKIGSHVAGLIEDGSTLQMGIGA
                     IPGGVLLHLRDKRHLGIHTELFSDGVIELYEAGVITNEKKTLHPGKMIAGFLLGTRRL
                     YDFVDNNPVVELHPTEYVNDSYIIAQNERMVAINSAIEVDLTGQVCADSIGTRLYSGT
                     GGQLDFIRGAARSKGGKPIIALPSITVTGSGKRFSRIVPTLKPGAGVVTTRNDVRYIV
                     TEYGVADLYGKSLRQRARALIDIAHPEMQEELEYEASKLKYIFERTAVK"
     gene            2808..3941
                     /locus_tag="VFQ23_26440"
     CDS             2808..3941
                     /locus_tag="VFQ23_26440"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9910212.1"
                     /translation="MNKMKANVFHGVNDIRVEEVEKPRAGFGEAVIRVTLTTICGTDL
                     HIVRGEYPVNPGLIIGHEPVGVIEELGPGVTGYEIGDRVLVGAITPCGQCRACLSGHL
                     SQCGHAGHNHGAGYHALGGWRFGNTINGAQAEYLLVPHAQANLAKIPDGVTDEQVVLL
                     ADIASTGFSGAESGGVKIGDAVVVFAQGPIGLCATAGAKLMGASLVIGVDGDNTRLEM
                     AKRMGADVVLNYNDMDVVAEVKRLTEGGADVTIEALGLQETFENALRSLRPGGTLSSL
                     GVYSGRLQVPYDAFAAGIGDYRIVTTLCPGGKERMRRLMSAVQSGRFDPTPLLTHSFS
                     LDEIAEGYRVFGERLDGVMKVAIRPFPSEKESILAGADKFAWQ"
     gene            4215..4640
                     /locus_tag="VFQ23_26445"
     CDS             4215..4640
                     /locus_tag="VFQ23_26445"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9910213.1"
                     /translation="MSNRIVPNWMTPNPIITSPGSKLSEAHRLMVEHNIAYLPVISHG
                     KRVGTFTKWDPHKTRRFDNRDFGVQHLQCMLERLTVREMMACVPVTVSPDATIEEAVG
                     LMLEHGLGILLVVEEGKLIGMITESDISLFILNVESYPS"
     gene            4878..5069
                     /locus_tag="VFQ23_26450"
     CDS             4878..5069
                     /locus_tag="VFQ23_26450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910214.1"
                     /translation="MDKSLALAIILDLETSLRMIDFALDEKDTILAKEALTNARLEVL
                     TLRSILEDRSLRSHQTLSS"
     gene            5196..5831
                     /locus_tag="VFQ23_26455"
     CDS             5196..5831
                     /locus_tag="VFQ23_26455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910215.1"
                     /translation="MNFFTRLHKFLLLILWGIGLTSCGVLSAAEPTVDVREVQTSVFF
                     TMQANLTSSAPPSTPAPVFTIGPTSTDVSPADYPSAVAARFQSLQASFVELIDIHTEL
                     SANPNMSRNMEWYAQAVAALVKVTNGAAEIARMNNYPPEYETFHQYMQNVAAEGNLLF
                     SNYMLALDNQDLTAQNQATSNLSKMITELNQASTELNRLNPAGLPAPTVVP"
     gene            5965..6186
                     /locus_tag="VFQ23_26460"
     CDS             5965..6186
                     /locus_tag="VFQ23_26460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910216.1"
                     /translation="MDNQPLELIRLLLARLERISVDSYWAHRASGLRGSLLRALEKLE
                     AGRPLQTSKLEALIATGFDILEEAAREKS"
     gene            6208..7320
                     /locus_tag="VFQ23_26465"
     CDS             6208..7320
                     /locus_tag="VFQ23_26465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9910217.1"
                     /translation="MPNEISIRHIQSLEEFEALRSQWNHLLEGRQRKTSFLAWEWLSA
                     WWTHYQEHRELWLITAWVDDVLVGAAPLMKTNIRKYGLRFRLLHSLGAPNCDESDFLV
                     PNGDFKIVKALCDYLFSRRAHWDAVELNEFESKTSNWQLIRNYFFNTGYIIQADTNYH
                     YHIVIKESWEKYWKSLSKNLRHNIERRLRRAQESHQVEFRYIKGKDLKWIDFETLFRV
                     SEKGNFPEKYKSDQERGFQRELWHRMQDHDWIEMAFLCLNGQAVAFEYGFNIDGSFED
                     WRTGFDLDHSELAGGTLLLYYLLKQFHGDGHRDLDFLRGEYDFKEKWLPEKREFIRLR
                     IIQPHRLSARLALLVLPNLWRWWKSKSQQKKKSRQV"
     gene            7317..8414
                     /locus_tag="VFQ23_26470"
     CDS             7317..8414
                     /locus_tag="VFQ23_26470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9910218.1"
                     /translation="MNSDLHIKQIRDVDSFANLKEQWQQLLSASPVQSAFLSWEWLYS
                     WWKTYGEKRQLRLITLWNGTELRGIAPFMLERKAKFGVGLRILKSLGTPQSDVGGFIY
                     RAGDLDVVDRLIDFLITNQSEWDLIEFHEYVDSCPERIAFKQQFTRNGSPIVEEVKPH
                     YYLPLEGSWESYHKSLPKKFRYNLGRAMRLAEDIGPVECRHFTGSDMNEGVIQTIIEI
                     NRYSHFPRLYNMPMEQVFLKTLAEQMKHSRWFGAFILYVDRQPVAYEYGFHFKGSFED
                     WRAGFDTRLSSTISIGKALASMVIQACYAQATVEIDFLRGDEPYKLEWKPLACEYANT
                     RVFNPRAPGAIAAYQWLVNIKPHLKKFSSRK"
BASE COUNT         2222 a         2024 c         2156 g         2180 t
ORIGIN      
        1 ggtgtgagac ggttgaccca agatgggcat cactgcctgg tcgaacgtaa cgccggctta
       61 ggagctggct tctcagacaa tgactatcta gccgcaggtg cacagatcat ttacgctggt
      121 gaagaaattt atggtcgcgg cgatatgatc ttgaaaattg cccgccccac tctggaagaa
      181 atttcctgga tgattcctcg acaaacatta ctgggtttcc ttcatttgca ttcggctcag
      241 cccaacaaaa tacagctgat gttggagaaa cacatcacag ccatctcgta tgagcaaatc
      301 gaatatcaag atggcactcg acccgtcttg aaaccactga gtcaaattgg ggggcaaatg
      361 gccgcacaaa tggccgcaca aatcttacaa aataattcag gaggcaatgg tgtcctgctc
      421 gggggggtgc ccggcgttcc gccagcggag gttgtgatca tcggggtagg agttgtggga
      481 gagtctgctg cccgcgcttt tctgggaatg ggcgcgcatg taactcttct cgatagcgat
      541 ccggaccgat tgcaagcggt agctgtgagt ctcccaaggc atgtaatttc cctggcgtca
      601 aatccactga atattgaacg aacctgttcc ctggcggatg tagttgtggg cgcagtgcat
      661 atggaccacg aacgggctcc tgtcatcatc acgaaagaga tgatcgaaaa aatgcgcact
      721 ggctcggtat tcctagacct gagtgtcgat gaagggggct gcgctgaaac atctcgtccg
      781 actacccatg acaacccgac ttacacggtc gatggagtga tccattgttg cattccaaat
      841 ctgtccggag tagtagctcg cacagcaact ctcgcttttc agaacgcagc ctggcacttt
      901 atcgagcaaa tctcgagaca cggtgtaaga gatgtgatgt caattgatcc ggctcttgcg
      961 agcggcgtct ccatggatcg cggaaaacta cagaatcccg agcaatacct tgatatcccc
     1021 acacgaggtt taagatgaac tggaacgacc tgtacaacca acgcgttaca tctgctgaag
     1081 tagcggtaaa gcaaatcaat tccggaatgc gcgtttttct atcagggaac tgctccgtgc
     1141 cgcaaacggt cctcgcagct ttggttgccc gagctcatga cctgcaggat gttgaaataa
     1201 tccaggttct tagcataggg ggcgcagact atgtttcacc agacatggca ggacatttac
     1261 gaatcaacac cctgttcatt agtgacaatg tgcgggaggc ggtcaatgac gggcgtgcag
     1321 actttacccc tgcccatctt tcggagattc cccgtttgtt ttcgtccggt gtccttccta
     1381 ttgatgttgc cctcattcag gtctcgcccc cggacgagca tggctattgc tcttttgggg
     1441 tggaagtcgg cgtcacaaaa accgcagctc aatatgcaag gattgttatc gcggaagtca
     1501 acccacagat gccacgcacc ctcggagact cttttatcca tgtcagcaaa atccatcaaa
     1561 tcgtccctgt gcattatccc ttaccagaag tttccatggg tgaacctacc gaggttagta
     1621 agaagattgg aagtcatgtt gccgggctga ttgaggatgg ttctaccctc caaatgggca
     1681 tcggtgcgat accagggggc gtccttctcc accttcgcga caagcgccac cttggaattc
     1741 acaccgaatt attttctgat ggggtgattg aactgtatga ggcgggagtg atcaccaacg
     1801 aaaagaaaac attacatccg ggtaagatga ttgccggctt cctcctaggg actcgccgat
     1861 tatatgactt tgtggacaat aatcccgttg tcgaattaca tcccacggaa tacgtcaatg
     1921 attcatacat catcgcccaa aacgaaagga tggtcgcgat caactcggcc attgaagtcg
     1981 atctgacagg tcaggtttgc gcggacagta tcggtactcg cctgtactct ggcacaggcg
     2041 gacagttgga tttcattcgg ggcgctgcac gttctaaagg aggcaagcct atcattgctt
     2101 tgccatcgat tacggttact ggctcaggga aacggttcag ccgtatcgtc cccacgctca
     2161 aacctggtgc cggagtggtt acaacgagaa atgatgtccg atatatcgta acagaatacg
     2221 gagtcgcaga cttgtatggt aaatccctcc ggcagcgcgc acgcgcacta atcgacattg
     2281 cccaccccga gatgcaggaa gagctcgaat atgaagccag caagctgaaa tatatttttg
     2341 aacgtaccgc cgtaaagtaa cgtgattttt aacaccgaag ttcaaatcaa tgtccattgc
     2401 tatcttagcc ctccttgtat tgattttgat cattatcatc tctgccatcc ggacggatct
     2461 gaacactgat gtattagcca tcgctcctca cctcaaacga tcaatgttgt gctggccgtg
     2521 acgatcgagt tggtttccat ttacagtagt tcgtcaggtg tggttacgcc agccttcatc
     2581 ccgctgctgc caggcctgat tcagacggct gttgttgtgg ggcatgtcta tgtcagtggc
     2641 cggagctctg ttagcgttta tgtttctgga tttgtgacca gtgaaatctt gtgacaaatg
     2701 tcacaaagaa agtgggacac gtgccccctg cttacaagca aactcatggg gataatggat
     2761 acgaaagaac tgttaaaagc ttcaatctca gtcccaagga gatcaaaatg aacaaaatga
     2821 aggcaaatgt ttttcacgga gtcaatgaca tccgagttga ggaggtcgaa aagccgcgcg
     2881 ccggattcgg cgaggctgtc attcgcgtaa cgcttacaac aatctgtggc accgatttgc
     2941 acatcgtgcg cggcgaatat ccggtgaacc ccgggcttat cattggacac gagccggtag
     3001 gggtgatcga agagcttggt cctggtgtaa cgggttatga aattggagat cgggtgcttg
     3061 tgggtgcgat cacaccctgc ggtcaatgtc gcgcttgtct atccgggcat ctctcacaat
     3121 gtggtcacgc gggacataat cacggtgcgg gctatcacgc cttaggtggc tggcgttttg
     3181 gtaatacgat caatggtgcc caggctgaat atctgcttgt tccgcatgca caagctaacc
     3241 tcgccaagat ccctgatgga gtaaccgatg aacaggtggt cctgctcgcc gatatcgcct
     3301 cgaccggctt tagtggcgcc gaatcaggtg gtgtgaaaat tggggatgca gttgtggtct
     3361 ttgcccaggg acccattgga ctttgtgcga cagcaggagc caaactcatg ggtgcttcgc
     3421 ttgttattgg cgtcgatggc gataatacac gactggaaat ggcaaaaagg atgggtgctg
     3481 atgttgtgct caattacaat gatatggatg tcgttgcgga ggtgaaacgg ctgacggagg
     3541 gtggtgccga tgtaacaatt gaggctcttg gcttgcaaga gacattcgaa aacgcattac
     3601 gctccttacg tccagggggg actctctcca gcttaggtgt ctattcaggc aggctgcaag
     3661 tcccctacga tgcctttgct gccggcattg gagattatcg gatcgtcaca accttgtgcc
     3721 ctggtggcaa agagcgcatg cgccgcctaa tgagcgccgt tcaatcaggc cggtttgatc
     3781 caactccgct tctgactcac tcgtttagct tagacgagat tgctgagggc taccgtgtgt
     3841 tcggtgaacg gttagatggc gtaatgaaag tcgctattcg accgttccca tcggaaaagg
     3901 agagtatcct cgccggtgcg gacaaattcg cctggcagta ggtgtgaata aaacggtctt
     3961 gatgtacatt cgtccatctt cttagtgaat gactgtggga ccacctaatc ctgccgaacc
     4021 cattccttgt gttagccata aactcccctc tgcgagggga gtttattttt tttattcata
     4081 ccaactgaac ggacctggca acattgggca tatcttccag ttcgttttgt gggtcatctg
     4141 tccccgagaa ttctggatat tcgacccttc atagttcagg gaaaacgagg ataatggata
     4201 tggagaatat tcttatgagt aaccgaatcg ttccaaactg gatgactcca aatccaatta
     4261 tcacttcgcc aggctcaaaa ctatctgaag cccatcggtt aatggtcgaa cataatatag
     4321 cgtacctgcc agtaattagt catggcaagc gggttggtac cttcaccaag tgggatcctc
     4381 acaagacaag gcgatttgac aaccgtgatt ttggtgttca acaccttcag tgcatgctgg
     4441 aacgtctaac tgtaagagag atgatggctt gtgttccagt tacggtttca cccgatgcaa
     4501 caatcgaaga ggcagtgggt ctgatgttag agcatgggct tggcatctta ctggtagtgg
     4561 aagaaggtaa gcttatcggg atgattactg aaagtgatat ctctttgttc atactgaatg
     4621 tggaaagcta cccttcgtag cgcaaaacag ggctgccaag tgcgttcata acaacccaca
     4681 cagaacgccg atcagatgta caataccata atattagatg cctataccga gaaaattcgg
     4741 agctgatagt tacacgtccg accccgcagc ttgcctaatt ctgtaatatc tattaatttg
     4801 tattttgttt gatgcattac cgtcagtggg acaagtatga ataaactcta accgcgcaaa
     4861 gcgcaacctg gaggacaatg gataaatcac tggccctggc tattatcctt gacctggaaa
     4921 cttcattgag aatgatcgat tttgccctgg atgaaaaaga tacaatcctt gcaaaggaag
     4981 cgcttaccaa cgcaaggctc gaagtgctga cgctgcgcag catattggaa gatcgatccc
     5041 tccgtagcca tcagaccctt agttcttgac tcgtattcac tttcaggcga caattccgta
     5101 ctcagacgtt tccttaaaaa tttcaaacaa acccgagctt ataagccagt cgaatatctc
     5161 ctgcaatcgg ttcctaaaaa agaatggaga aggaaatgaa ctttttcact agattgcaca
     5221 agtttctgct gctcattcta tggggaattg gtttaacgtc gtgtggggtt ctttcagcag
     5281 cggaaccaac agtcgatgtg cgtgaagtcc aaacttcggt atttttcacg atgcaggcta
     5341 acctcacaag ttcagcgcct ccgtccactc ctgcacctgt tttcaccatt ggtccgacct
     5401 cgaccgacgt ttctcccgca gactatccca gcgccgtagc agcgcggttt caatccttgc
     5461 aagcgtcatt tgtagaactg atcgacatcc atactgaact gtcggcgaac cccaatatga
     5521 gtcggaacat ggaatggtat gctcaggcgg tcgcggcgct ggtaaaggtt accaacggtg
     5581 ctgctgagat cgcacgtatg aataattacc cacccgaata tgaaaccttt caccagtaca
     5641 tgcaaaatgt agctgccgag ggaaatttat tgttttccaa ctatatgttg gccctcgata
     5701 atcaggatct taccgcccaa aaccaggcta ccagcaacct gagcaaaatg atcacggaac
     5761 ttaatcaggc gtctacggag ctgaacagac ttaatccagc aggtcttcct gcgccgacag
     5821 ttgtcccata gccaaccagt taatgtacta acctgttcag atagtgggtt gatctgactt
     5881 tcagagttgt aacttatcgg aggcttgacg ctgcttcact tcgctgtgag aatcaatcag
     5941 ccataagcta acggaagtta tcaaatggat aatcagccgc tcgaactcat tcgactcctg
     6001 ctcgcccgcc tcgaacgaat ctcagttgac tcctactggg ctcaccgggc aagcggacta
     6061 cgaggttccc tgctacgggc cttggaaaaa ctggaagctg gccgcccact ccaaacgtca
     6121 aagctcgaag cgttgattgc gaccgggttt gatatccttg aagaagctgc gagagagaag
     6181 tcctgatcgg aatgcgaaaa tcgcattatg cctaacgaga tatctattcg tcatattcaa
     6241 agcctggaag agtttgaagc actgaggagc cagtggaatc acctgcttga agggcgtcaa
     6301 aggaaaactt cgtttttggc ctgggagtgg ctctctgcct ggtggacgca ctatcaggaa
     6361 catcgtgagc tttggcttat cacagcgtgg gtagatgacg tactcgtcgg cgcagctccg
     6421 ctcatgaaaa cgaacattcg gaaatatggt ttgcgttttc gcttattaca ttccctggga
     6481 gcgccaaatt gtgatgaaag tgatttcctc gtaccaaatg gggatttcaa aattgtaaag
     6541 gccctctgtg attacctttt ctcgcgacgt gcgcactggg atgcggttga gctaaacgaa
     6601 tttgaatcaa agacatcgaa ctggcaatta attcggaatt attttttcaa taccggatac
     6661 atcattcaag ccgatacaaa ttaccactat cacattgtaa ttaaggaatc ctgggaaaaa
     6721 tactggaaaa gtctttcgaa gaatcttcgt cacaatattg aacggcgact tcggcgcgca
     6781 caggaaagtc atcaagtgga attcagatat ataaaaggca aagatcttaa atggattgac
     6841 tttgaaacat tgtttcgcgt gagcgaaaaa gggaattttc ccgaaaagta caaatcagat
     6901 caggaacgag gttttcaacg cgaactatgg catcgaatgc aagatcatga ttggatcgaa
     6961 atggcatttt tatgcctgaa tggacaagca gtcgcattcg agtatgggtt taatatcgac
     7021 gggagcttcg aagattggag gaccgggttt gatctggatc attcggaact ggcaggtgga
     7081 acactcctgc tctattatct cctgaaacaa tttcatgggg atggtcatcg tgatctggat
     7141 ttcttacgag gcgaatacga tttcaaggag aaatggctcc cggaaaaacg tgagtttatt
     7201 cggttacgta ttatccagcc gcatcgtctt tctgctcgcc tggctctact agtgctgcca
     7261 aacttatggc ggtggtggaa gagtaaatca caacaaaaaa agaaatcacg acaggtatga
     7321 attctgatct tcatatcaaa caaatccgcg atgtcgattc cttcgcgaac ctcaaagagc
     7381 agtggcaaca actgttatct gcaagcccgg tgcagagcgc atttctcagc tgggaatggt
     7441 tgtattcatg gtggaaaacg tacggggaaa agaggcagct tcgcctaatc acgctttgga
     7501 atggcaccga attgcgcggc atcgcgccct tcatgctgga gcgaaaagcg aaattcggtg
     7561 tcggactcag aatattgaaa agcctgggca caccacaaag tgatgttggc gggtttattt
     7621 atcgtgccgg tgacctggat gtggttgatc gcttaattga ttttctgata acgaatcaaa
     7681 gtgagtggga tcttatcgaa ttccacgaat acgttgattc atgtccagag cgaattgcct
     7741 tcaagcaaca attcacgagg aacgggtcac ccatcgtcga ggaagtcaaa ccacattact
     7801 atcttccgct tgaaggaagt tgggaaagct atcataagag cctacccaaa aaatttcgct
     7861 acaaccttgg gcgcgccatg cgcctggcgg aagatattgg tccggtcgaa tgcaggcatt
     7921 tcacgggaag tgatatgaat gagggcgtga tccaaacgat tatcgaaatc aaccgttatt
     7981 ctcattttcc acgcctatat aacatgccca tggagcaggt tttcctgaag actcttgctg
     8041 aacaaatgaa gcactcccgt tggtttgggg catttattct ctacgtcgac cgacagccgg
     8101 ttgcttatga gtatggattt cacttcaagg gcagttttga agattggcgg gcaggctttg
     8161 acacccggct atcatccacc atttcgattg gaaaggcgct ggcgagcatg gtcatccagg
     8221 cgtgttacgc gcaggcgacc gtggagatcg attttttgcg gggcgatgaa ccgtacaaac
     8281 ttgaatggaa accactggct tgtgagtatg caaatacgcg tgtgttcaat ccgcgtgccc
     8341 ctggagcgat tgctgcgtat cagtggctgg ttaatattaa acctcacttg aagaaattta
     8401 gctcgcggaa gtaattgcat gaagaaaaaa gaaagtcgca cgatccatcc aattgtgtat
     8461 gttgcattaa ttctgttgac aattaatgca atctttcttg gaatgttata tttccagcgg
     8521 gaccgtccag gcgtgcaaat aacaaacaca tcggcggctt ctgccgctcc tcaggaatca
     8581 tc
//
LOCUS       DASTJP010000908        13666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U1571
            k141_1044463, whole genome shotgun sequence.
ACCESSION   DASTJP010000908 DASTJP010000000
VERSION     DASTJP010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969985
            Sequence Read Archive: SRR25158527
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:00:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,253
            CDSs (with protein)               :: 5,253
            Genes (RNA)                       :: 41
            tRNAs                             :: 38
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13666
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044463"
                     /isolate="SMAG_U1571"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.06444444 N 120.1038889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="VFQ23_26475"
     CDS             complement(<1..1516)
                     /locus_tag="VFQ23_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9910219.1"
                     /translation="MYRVESLMSARLFAVPQHADNRVFFLSNESGKLSLYAMNAGGSV
                     PEPLLPPNIALQNPHLIGGLSFYVFSKLKKILVMIDQDGDENYQPMLIPTDGGFPEPA
                     FKNFFAEYRVHLGECDAEKNIAYLNAERRDKPLIETYRADLKSGKVTKIAESEFGFGV
                     GHHDKGHKKLLLGTGYTVGDSVLYLSSGGKHKVIYGKPLENRAAGESVPLNGIGSSVF
                     SPSEKGAVVITSLFDDTYSLGYVDFRKPGQMEEVQVKGIKHKGVGEMVELVHLKKDRY
                     MITYNIDGCSWVYEGEFNERKRLMTLKYTLVGLKPLDNGMLEHVDYDEANDVFTFSFS
                     TATSPTQIYTIEGRKRNKVMRHTNEKILGMQDGVLSAGEDASFISHDGLRISARLYLP
                     SKGLGFKGPRPVVYYIHGGPQGQERPDFAWFSMPLIQYLTLRGFAVFVPNVRGSTGYG
                     LSYTKHIDRDWGGKDRLDHVHAMTEILPKDKRVDASRAAVVGRSYGGYMTLMQAG"
     gene            complement(1603..2667)
                     /gene="mnmA"
                     /locus_tag="VFQ23_26480"
     CDS             complement(1603..2667)
                     /gene="mnmA"
                     /locus_tag="VFQ23_26480"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET9910220.1"
                     /translation="MNKVVVAMSGGVDSSVAAALLKEQGYDVIGMMLRLWSEPGKEDS
                     NRCCTPDSMAQARRVAAKLDIPFYVIDAKDVFHDTVVQYFLDGYARGETPNPCLICNR
                     QIRWTFLLNHALALGADFMATGHYVRIKKNESGLSQLLRAVDRAKDQSYVLHVLDQEK
                     LKHALFPVGDYSKPEIRQIAEKYGLPTATRKDSQDLCFLAGEDYRNFLQRNAVEMLNA
                     GEIMTASGKSIGQHTGLANYTIGQRKGLGVASSVPLYVIRKNAETNQLIVGTQEELGS
                     KELTARDVRWVSGEVPPDTFRAEVKIRYTAKEAEATVRPLNNGQQVHVEFDAPQRDIT
                     AGQAAVFYQGDVLIGGGIIV"
     gene            complement(2692..3846)
                     /locus_tag="VFQ23_26485"
     CDS             complement(2692..3846)
                     /locus_tag="VFQ23_26485"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET9910221.1"
                     /translation="MIYMDYSATTPVDARVTDAMSPYFSDSFGNPSSVHRYGQVAESA
                     IDSARESVASVLNCRPDEIIFTSCGSESDNLAIRGAAYAMREKTGAKWILASRTEHPA
                     VTKTLLHLEKYEGFLIEWLDVDQHGMVTPEIVAKAICNNTAIVSVMYANNEIGTINPI
                     QEIAEICRANGILFHTDAVQAAAYLPVDVQVLGVDMMSLGAHKFYGPKGVGALYVRKG
                     TPLISHLTGGGQEFSLRAGTQNVPYIVGFAESLRLANEESPQRVAHVRPLRDKIIGTV
                     LETIPDSQLTGDLESRRPHHASFAFKAVDGNLLLTLLDSAGFACSSGSACKTGNPEPS
                     EVMNAIGLSRDWGLGSLRVTLGRDTLPEHVESFLAVLPSLVEKARQLNYN"
     gene            3955..4689
                     /locus_tag="VFQ23_26490"
     CDS             3955..4689
                     /locus_tag="VFQ23_26490"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HET9910222.1"
                     /translation="MIKFRLVTRAQTLVIISVLLLSACGATPAEVTTPTVDANAIRTE
                     AVSTFIADLTLTALAAPPTNTPVSTVTPAPTFPPLATSTGGTPFGSAPTTAPVGGTTG
                     CYGLRFVSDVSVPDNTTMTPGQTFTKTWKVLNAGTCAWDAGFKFAFVGGEQLNGTTQT
                     LSASVAAGATFDISVPMTAPTNRTGTLRGNWRMSTASGQFFGDEVYVQIVVGGSGPTS
                     TGGAPTSTNTTGAPAVTDTATATATP"
     gene            4794..5474
                     /locus_tag="VFQ23_26495"
     CDS             4794..5474
                     /locus_tag="VFQ23_26495"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9910223.1"
                     /translation="MIVALFDSDGTLYSNQMGWGMMKYEEINGRKNRARLYYASLMPS
                     YFLNKAGLIKPESFQHTLILGLTRFFRGSTLEDGMSLFKWVVNEYLLPATRADIAQRL
                     KEHQERGHRVVIISGSFTPCLNLIGKHFGVTDLIGTRVEVQNERYTGNIIPPLITGAA
                     KAEKIRELLSTYKDDVDWSSSYGYGDSFTDRDMLGLVGHPVAVYPDKKLHKLAKEKNW
                     EVLGTPKE"
     gene            5705..6088
                     /locus_tag="VFQ23_26500"
     CDS             5705..6088
                     /locus_tag="VFQ23_26500"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9910224.1"
                     /translation="MFESMSKNKALMRRIYEEMWNLSDPSIAVEIFERPEGVERFVSQ
                     FLRAFPDLQHTIEGMIEEDAHVVVQFSAHGIHKGQWLQYPPTGRSIHYTGVTVAQIEG
                     DKVVKHHTWWDKLGLVEQILTEPIP"
     gene            6223..7233
                     /locus_tag="VFQ23_26505"
     CDS             6223..7233
                     /locus_tag="VFQ23_26505"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9910225.1"
                     /translation="MSKRMIKTLADLAELAGVSKSTVSRALNDSPLIGEETKSRIRTL
                     ARQHNFQINAPARQLSMKQSRTIAFVTHAYHKDFSVADLFGLEILGGVSNGLAQQGYD
                     LLIIHVDPYDTKWARQYFDTGRVDGFILMTSSKKQTHVKALAEIGAPFIIWGVALPNQ
                     KHCAVTGDNFSGGRLAADHLIAVGRNKIGFIGGPSYELEVQHRLEGYKSALQTAGMNI
                     ESDLIEYGDFSNTSGAEAMYRLLEKQPDLDAVFVNSDLMAIAAMDAIRETGRQVSGDI
                     AVVGYDDLSIAEHSNPPLTTIRQNIPLAGKLLAQNLIQYIQSGLTTNVSIPVELVVRK
                     SA"
     gene            7322..7840
                     /locus_tag="VFQ23_26510"
     CDS             7322..7840
                     /locus_tag="VFQ23_26510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910226.1"
                     /translation="MNLYPTITNLRSYVSYQFSALRIRAARDRFFAKLMGKNASLLTF
                     PDELQHGKGSKKPVGVKNIRVDQITGTLHQCKDFDHKFRPIQKHLLARWANTFISLNH
                     DQWSPILVHKLGEQYFVEDGHHRVSVARAVGMEFIEAKIWEYSPSPKPAERSVQPVAC
                     AEPSSSKSYAVR"
     gene            8008..9828
                     /locus_tag="VFQ23_26515"
     CDS             8008..9828
                     /locus_tag="VFQ23_26515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9910227.1"
                     /translation="MTIYRTPRRFLNSHFGAAIIVTIFVALIVVISVRAVQVLRQAAT
                     GAVIKVPGDYSTIQAAIDAAKPGDIVQISAGIYNENLSLNKPVTLVGESFDQVNPVNN
                     LSIINGGSAAPTIVIPAGLTQMPTIRGLVIQSGIDGIQATSPFIAEYNYFHSAANLVS
                     YQTGGGGFNRGNIYFNATDNAIRLDNIDRPLLIESNRILYSGDDGIEISLQNTVVPPA
                     LIEIDIWNNMILGNREDGIQFVDHPGDPQDTNRRFVIAGNLIANSRKAGIGLMPNANT
                     LEDYSGGDVIEAIRVFQNTFYGNDYGISGGDNLVAFNNIIASSINVGAWKIQGGNSTV
                     AYTLFHNNRIDADQSTLGVGNIAGQDPLFVAAPNPGPDGAWETVDDDFSGLVLRTGSP
                     AIDKGVTQYVASNGEPIPPNPITGYTGAAPDLGWREFGAPIFLTPTATLISSLTPLPT
                     FTPMTATPTSTMTATPPATGTPITPTVTPITPTVQSTNTVTAPSPTTIPATATVAVTS
                     SSLEIQGVTPNSAQADTVVALNIVGAGFQNGSVVTFEGGAGGNQEITSVQVVSPNLIV
                     VNMTARNDGTAGTQVWDVRVTNPDASSAVLTDGFTIYPIP"
     gene            complement(9932..10504)
                     /locus_tag="VFQ23_26520"
     CDS             complement(9932..10504)
                     /locus_tag="VFQ23_26520"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET9910228.1"
                     /translation="MISIVQYKPSWSNEFLEKGTLLRQFLDDLALRIDHIGSTAVPGL
                     AAKDVIDIQITAERLTEKLERAMNLAGYQRLVHINQDHVPPGKESEAYQWEKWFFQPT
                     SPGRKVNAHVRLPGRANQRYAILFRDYLRAFPTISQAYGQVKEAIVKYHPEQDMEAYY
                     DIKDPVCDIIMGGAEEWAASTNWQQGPSDC"
     gene            complement(10629..11459)
                     /locus_tag="VFQ23_26525"
     CDS             complement(10629..11459)
                     /locus_tag="VFQ23_26525"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9910229.1"
                     /translation="MRLKRTSFFVGMILLISFSIPAFVAGALNVKPQQVTFVNSQGIR
                     LTGRVFKPDGNGPFPAVVMMPGCMGAYAYGTGAKEISSMYLEWGDRLVTAGYVALLVD
                     SQDQCGNSTTGRSEINIRPYDAYAGLNYLVTQSYVAADKVGVMGWSHGGSSVMAAMDE
                     SQSVDEAQFKAAVAFYPGCTFGNALGVLKHSTWKPYAPLVIVQGSVDAITDPAACTAR
                     VTGAQQLGVTNVTINTFEDAQHRFDGATKAEDGFTQLDVDAKIAADAQVLHFFETHLP
                     "
     gene            complement(11698..>13666)
                     /locus_tag="VFQ23_26530"
     CDS             complement(11698..>13666)
                     /locus_tag="VFQ23_26530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9910230.1"
                     /translation="HILPLYEYGSEKNVRFMVSPYIPGGTLTARIKSPLSFEQVIKYG
                     TEIASALDYLHQQGVIHRDLKSSNILLDLSDHTYLADFGLARIISDSTLAFHTGHGTP
                     PYAPPEQIRSKEITAKSDIFSFGILLFEMLTGQLPWNGKRQLGVEQLHSDQQLPDPRE
                     YITGLPPLITDVLRRVTSANPDLRPRSGSEVMRMMHYVFNLPADSMSSKRPYDGSTSR
                     SKDAEEILNSGLTQWESTDGKHNLGLTKFALINAEGIKSDTDVFNRFMLSQALTYGHY
                     DDEWWSIVRNPRERLMVSSALLRKENEAITGRVLDRLTGDRHILSSSNGLPKNLANSL
                     LALGTKTNDAILRQKIFTGLRILLRPGSVWKDPELDSNHIKRLGVLALEDSEFGDSAA
                     ELIGHIRSPSAVRVVLTHSNEGRKIDALLLIQKVAGSLPSLVPGPVRFRLSLERNLQR
                     MTQRPVNLIGAYGTIFLGSALGVSLQVYLTYNLPTFMDVARIVASLFRGLIIGSILGL
                     GIFLTRVIVERFHSSNTYLSLMLGTILGGLGINIAIFIFHMLFLGTPPQGVLITLGSL
                     LIAFTFAIGGLIRPKLVKMLLSSVSVFISILGTWLIHLNLAASPVDLTPFFRYDPAWP
                     LIQVVFTALSVSFLIGIFGNLIDLSLRNEHV"
BASE COUNT         3606 a         3466 c         3243 g         3351 t
ORIGIN      
        1 gaccagcctg catcagggtc atataaccac catatgagcg gccgaccacg gccgcacgcg
       61 aggcatccac ccgtttgtct ttcggtagga tctccgtcat cgcgtggacg tggtccaggc
      121 gatcctttcc accccagtca cggtcaatgt gcttggtata cgagagaccg tatcctgtgg
      181 atccgcgaac gttgggcaca aaaacagcaa atccacgaag tgtgagatat tgaatgagag
      241 gcatcgagaa ccaggcaaag tccgggcgct cctgtccctg tgggccaccg tggatgtaat
      301 atacgaccgg gcggggacct ttgaagccga gtcctttcga tgggagatac agccgggcag
      361 aaatgcgcaa gccatcatgc gaaatgaacg acgcgtcctc tcctgcggag agaacgccat
      421 cctgcattcc caggattttt tcgttggtgt gccgcatgac cttatttcgc tttcgacctt
      481 cgatggtata gatctgagtc ggcgaggttg cagtagagaa cgagaatgtg aatacatcat
      541 tcgcttcgtc atagtcaaca tgctcgagca tgccattatc gagaggtttg agtccgacca
      601 aggtatattt gagagtcatc agtcgcttcc gctcattgaa ctccccttca tacacccagg
      661 aacaaccgtc aatattgtag gtgatcatgt aacgatcttt tttgagatgg accaactcga
      721 ccatctcacc cacacctttg tgcttgatcc cctttacttg cacttcttcc atttgacctg
      781 gttttcgaaa gtcaacatat ccgagactat aggtatcatc aaataaggag gtaataacaa
      841 ccgcgccttt ttcgctcggc gaaaagacag aggatccaat accgttgaga ggaacgcttt
      901 cccccgccgc acggttctcc aggggcttgc catagatcac cttgtgcttc cctccactcg
      961 atagatacaa aacactatcg cccacggtat acccagtacc caggagcagt ttcttatggc
     1021 ctttatcatg atggccaaca ccgaagccaa attcgctttc agcaattttt gtgactttgc
     1081 cactctttag atctgcgcga taggtttcga tcaaaggctt atcacgacgc tccgcattta
     1141 gataagcgat atttttctca gcatcacatt cgccaaggtg aacgcgatat tccgcaaaga
     1201 aatttttgaa cgccggctct gggaacccgc catcggtggg aatcagcatg ggttgataat
     1261 tctcatcgcc atcctgatcg atcattacta aaatcttttt tagcttcgag aaaacataga
     1321 acgagagccc gccgatcagg tgcgggtttt gcaaagcaat atttggtgga agcaatggct
     1381 caggtacact gccaccagca ttcatcgcat atagacttaa cttcccagat tcattgctca
     1441 aaaagaatac acggttatcc gcatgctgcg ggacagcaaa taaacgagca gacatcagtg
     1501 attcaacacg atacattctt caaactcctt aaaaatgaat tttgattttc tattttttta
     1561 ctaaaactgc aggacgaacc agcactgcgg ctatacgcaa aatcaaacga tgatcccgcc
     1621 gccgatgagc acatcaccct ggtagaaaac tgcggcctgc ccggcggtga tatctctttg
     1681 tggcgcatca aactcgacgt gtacctgctg gccgttgttc agcggacgaa ctgtcgcctc
     1741 cgcctccttc gccgtatagc ggattttcac ttccgcacgg aacgtatcag gcggcacctc
     1801 tccactcacc cagcggacgt cgcgagcagt taactccttt gatccaagtt cttcctgcgt
     1861 tcctacaatc aattgattgg tttctgcgtt tttcctaatt acgtataacg gcaccgaaga
     1921 ggcgacgccg agtccttttc gctggccgat cgtgtaattt gccaatcctg tatgttggcc
     1981 aatggatttg ccggaggcgg tcatgatctc gccagcgttg agcatctcca ccgcgttacg
     2041 ctggagaaag tttcggtaat cctctccggc cagaaaacac aaatcctgcg aatcttttcg
     2101 cgttgcggtt ggcaagccgt atttctccgc gatctgacga atctccggct tgctgtaatc
     2161 tcccaccgga aataacgcat gcttaagttt ttcctgatct aacacgtgca ggacgtacga
     2221 ctgatcttta gcacgatcca ccgctctcaa taattgactg agtccacttt catttttctt
     2281 gatcctcaca taatgcccgg ttgccataaa gtcggcaccc agagccagag cgtgattcag
     2341 tagaaacgtc caacggatct ggcggttgca gatcaaacag ggattgggcg tctcgccgcg
     2401 cgcatatcca tcaaggaaat actgtacaac cgtatcatga aatacatctt tcgcgtcgat
     2461 cacataaaat ggaatgtcaa gctttgcggc cacacggcgg gcctgcgcca tcgagtcggg
     2521 tgtgcaacaa cgattggagt cttccttgcc aggctcgctc cacaaacgaa gcatcatccc
     2581 aatgacatca tagccctgct ccttgagcaa ggctgcggca acagaggagt ccactcctcc
     2641 agacatagca acaacgactt tattcatcaa gtaattggtt ttgtatctaa tttagttgta
     2701 gttcagctgc ctcgcctttt caacgaggga gggtaaaact gccagaaaag attcgacatg
     2761 ctcaggcaat gtatctctcc caagcgtcac gcgcagggag cccagtcccc aatcccgcga
     2821 taatccaatg gcgttcatca cttcgctggg ttcagggtta ccggttttac aggccgaacc
     2881 agaggaacac gcaaagcctg cagaatcaag cagggtgagc aacaaatttc catcgaccgc
     2941 tttgaacgca aaactggcat gatgaggacg acgcgattct aagtcacctg tcagctggga
     3001 atcaggaatc gtttcgagca ccgttccgat gatcttatcg cgcaacggtc gaacgtgagc
     3061 cacgcgctgc gggctctctt catttgccaa tcgcagggac tctgcgaatc caacgatata
     3121 tggcacattt tgcgtcccag cacgcaggct gaactcctgc ccaccgcctg tcaggtgtga
     3181 gatcagcggc gtccctttac gaacatacaa cgcgcccacg cctttcggtc cataaaattt
     3241 atgcgcgcca agcgacatca tatctacacc taatacctga acgtccactg gcaaataggc
     3301 cgcggcttga accgcgtccg tgtggaataa aataccattt gcgcggcaaa tctctgcaat
     3361 ctcctgaatg ggattgatcg tgccgatttc attattggca tacattacgg atacaatcgc
     3421 ggtattatta cagatagctt ttgctacgat ctctggcgtg accatgccat gctgatctac
     3481 atccagccat tcaatcagaa aaccttcata tttttcgaga tgcaataaag tctttgtcac
     3541 cgccggatgt tctgtgcgtg aggccagaat ccattttgca cctgtctttt cacgcatcgc
     3601 ataagctgca ccccgaatcg caaggttgtc tgattctgaa ccgcaggatg taaagatgat
     3661 ttcatcgggc ctgcaattca ataccgaagc gactgactcg cgcgcagaat cgatcgcaga
     3721 ctcagccacc tgtccataac ggtgcacaga cgaagggttc ccgaacgaat cggagaaata
     3781 aggcgacatc gcatcagtga cgcgcgcatc tacgggcgtt gttgctgaat aatccatgta
     3841 aatcatagcc gctagattat agcatggtga gaataggaac attgtagggg tcctatccgt
     3901 taatcaaacc gaaaaagaca ggtaaaatat tgcgtctctt tgaaaggagt gttaatgatt
     3961 aaatttagat tagtgacccg tgcgcaaaca ttggtcataa taagcgtgtt gttgttatcc
     4021 gcctgcggag caaccccggc tgaggtaaca acgccgaccg tggacgcgaa cgcaatccga
     4081 accgaggccg tttcgacgtt tatcgccgat ttgaccttga ccgcgctagc cgcgccgcca
     4141 accaatacac cagtttctac ggtgaccccc gccccaacat tcccgccact tgccaccagc
     4201 acaggtggaa caccatttgg cagtgcacct actacggcac ctgtaggagg aactaccggt
     4261 tgttatggat tgcgctttgt cagcgatgtc agcgtccctg ataacacaac gatgacaccc
     4321 ggtcagacct tcacaaagac atggaaggtt ttgaatgccg gcacatgtgc ctgggatgcc
     4381 gggtttaaat ttgcattcgt gggtggcgaa caactgaacg gcactacgca aaccttatct
     4441 gcgtcggttg ctgctggagc cacattcgat atttcagttc ccatgacagc tccaaccaac
     4501 aggactggaa ccctgcgtgg caactggcgt atgtccaccg cttcggggca attcttcggc
     4561 gatgaagtat atgtccaaat cgttgtggga ggcagcggac ctacttccac tggcggcgcg
     4621 ccgaccagca ccaacacaac cggcgcaccc gcagtcacgg atacagccac agcaacagct
     4681 acaccataat tatctggtcc cttcttcacc gaagggactt ttgttatata cagatttggc
     4741 acagctaaat ctggtgttat catcattgaa aaaactacac aatagaggca tcgatgattg
     4801 ttgcactatt cgacagtgac ggcaccctgt attccaacca gatgggttgg ggtatgatga
     4861 aatatgaaga aatcaacggg cgaaagaatc gggctcggct atattacgca tccctgatgc
     4921 ccagctattt tttgaacaag gcaggattaa ttaagccaga gagtttccag cataccctca
     4981 ttctgggatt aacgagattc tttagaggat ctactttgga agatggtatg tcccttttta
     5041 agtgggttgt gaacgaatat cttttacctg ccacgcgcgc cgacatcgca caaaggttga
     5101 aagagcacca ggagagagga caccgagttg taatcatttc gggatcgttc actccttgtc
     5161 ttaatcttat tggcaagcat tttggagtta ctgatcttat tggcacacgg gttgaggtac
     5221 agaacgaaag atatacagga aatattatcc cgcccctgat tacgggagcc gcaaaggctg
     5281 agaagatccg cgagttgctg tcaacgtata aggatgatgt agattggtcc tcgagctacg
     5341 gatacggcga ctcgtttacg gaccgggata tgctgggctt ggtgggtcat cctgtagcgg
     5401 tgtacccgga taagaaatta cacaaactcg caaaagaaaa aaactgggaa gtattgggaa
     5461 cgccaaaaga gtaatggcaa ggtttgtccg tcaaaggcct tgacaacctc cccgtagcag
     5521 gggtactatc tcgaaactct gcactccttg tgtagagttt tttaatccct aacccaccaa
     5581 ttcaatactt tcgttttccg actgtgttct atgcacagtc ggaagattcg ttcaggttag
     5641 cacacgttaa cttacccgac tcacgttcgg gtcctttcct tttccacaca aaggagtgtg
     5701 aaccatgttt gaatcgatga gtaagaacaa agccctgatg cgccgcatct acgaagagat
     5761 gtggaatctg agcgatccgt ccattgctgt tgagatcttc gagagaccag aaggtgttga
     5821 acgattcgta agtcagtttc tgcgggcgtt cccagatttg cagcatacga ttgaagggat
     5881 gatcgaagaa gatgcccacg ttgttgtgca gttctctgca catgggattc ataagggtca
     5941 atggctgcaa tatccgccga caggaagatc gatccactat acaggagtga ctgtggcaca
     6001 aatcgaaggc gacaaggtcg tgaaacacca cacctggtgg gataagttgg gtttggtgga
     6061 gcaaatcctg acagagccga taccgtaaca gacctatatc acgctgttgg agtaagaagg
     6121 cggatgggtt ttctcgtccg tcttcgactt tttattttcg acccttgaca ctccaaaaat
     6181 ggagatatat aatgcaatcg gttgcatgaa acagaagatc ttatgagcaa acggatgatc
     6241 aaaacactcg cggaccttgc tgagttggct ggagtttcca agtcaacggt gtccagggcg
     6301 ttaaatgaca gtccgcttat cggcgaagag accaaatctc ggattcgaac gctggcacga
     6361 cagcacaatt ttcagatcaa cgcccctgcc cgacagctca gcatgaagca gagtcgcaca
     6421 attgcttttg tgacacatgc gtatcacaag gacttctcag tcgctgacct atttgggctg
     6481 gaaatcctgg gtggtgtttc gaatggactt gcccaacagg gttacgattt attgataatc
     6541 catgtggatc cttacgatac gaagtgggcg cgtcagtatt ttgatactgg tcgcgtggat
     6601 gggttcatcc tgatgacttc cagtaaaaag caaacccacg taaaggcctt ggcagaaatc
     6661 ggagcgccgt tcattatttg gggtgttgcg ctcccaaatc agaaacactg tgctgtcacc
     6721 ggagataatt tcagcggcgg aaggctggct gctgatcatc tgattgcagt tggaagaaat
     6781 aaaattggct tcattggcgg accatcctac gaactggagg tgcaacatcg acttgaaggt
     6841 tataaatcag ctttgcagac ggcaggcatg aacatcgaat ccgatttgat cgaatacgga
     6901 gatttctcta acacatctgg cgcggaggca atgtatcggt tgctggaaaa acaacccgat
     6961 ctcgatgcgg ttttcgtaaa cagcgatctg atggccattg ctgctatgga tgccattcgc
     7021 gaaacaggtc gccaggtttc aggtgacatc gccgttgttg gctatgatga tctgtctatc
     7081 gcggaacaca gcaatccgcc tctgaccacg attcgacaaa acattcctct ggcaggcaag
     7141 ttgctcgctc agaatctcat ccaatacata cagtcgggtc tgacgacaaa tgtatcgatt
     7201 ccagttgaat tagtcgttcg aaaatctgct taacattcaa tctctttttt tgaccgttta
     7261 tgcaaccgat tgcataatct cattcaaacc ttatcgaaac gaaccctgaa aggaaaataa
     7321 aatgaacctc taccccacca tcactaatct tcgcagctat gtttcgtatc agttttcggc
     7381 attgagaatc cgtgctgcac gagatagatt ctttgcaaag ttaatgggca aaaatgcgag
     7441 tctgctcacc ttcccggatg aactacaaca cggtaaaggg agcaaaaaac ctgtgggcgt
     7501 gaaaaacatt cgagtcgatc agatcacggg aacactacat cagtgtaaag atttcgatca
     7561 caaattccgg cccatccaaa agcatttact ggctcgctgg gccaacacat tcatcagcct
     7621 aaaccatgat caatggtcac ccatcctggt ccacaagctc ggagaacaat atttcgtgga
     7681 ggacgggcat catcgtgtct ctgtcgcgcg agccgtgggc atggagttta ttgaagcaaa
     7741 aatctgggaa tactcccctt cgccaaaacc agcagaacgc agcgtgcaac ctgtcgcttg
     7801 tgcagaaccc agctcatcaa aatcgtatgc agtaagataa attgccagga tatgcgactt
     7861 aaagcggacg agcagctcgt ccgctttttt catgtaacgc gcttgatgag aatttgatta
     7921 agtacttgag tacgcggaaa ttagcacctt tgacccatgc agttctgatg ttcgcatcgt
     7981 tttatgtatt aacactggag gtacatcatg accatatata gaacaccccg gcggttcctt
     8041 aactcacatt ttggtgccgc cataatcgtg accattttcg ttgcattgat cgttgttatt
     8101 agcgtccgtg cagttcaagt gttgcgccag gctgcaaccg gcgccgtgat caaagttcct
     8161 ggcgattatt cgacaattca ggcagccatt gatgcggcga aaccgggaga tatcgttcag
     8221 atcagtgcag gtatctataa cgaaaacctg agcttgaaca agcctgtgac gttggttggc
     8281 gagtcgtttg accaggtcaa cccggtcaat aacctgagca ttatcaatgg tggtagtgca
     8341 gcaccgacga tcgtcatccc tgctgggctt actcaaatgc ctactatacg agggctcgtc
     8401 atccaaagcg gtatcgatgg aattcaggct acttcccctt ttatcgccga atataactac
     8461 tttcattcgg cggcgaatct ggtctcttat caaactggcg gcggaggttt caatcgcggc
     8521 aatatctatt tcaacgcgac cgacaacgcc atccgcctgg ataatattga ccgccctctt
     8581 ttgattgaga gtaatcgtat tttgtacagt ggagatgatg gcattgagat cagtctgcaa
     8641 aacacggttg tcccgcccgc gttgatcgaa atcgacattt ggaataacat gatcctggga
     8701 aaccgggaag atggcattca atttgttgac catcccggag acccgcagga tacaaatcgc
     8761 cgttttgtca ttgctggcaa tctaattgcg aacagcagga aggctggcat tggactgatg
     8821 cccaacgcaa acacgctgga ggattattct ggtggagatg ttatcgaagc gattcgtgtt
     8881 ttccaaaata cattttatgg taatgactac ggcatctcag gcggcgacaa tttggtcgcc
     8941 ttcaacaata tcattgccag ttcgatcaat gtcggcgcct ggaagataca agggggcaac
     9001 tcaaccgtcg cctatacttt attccacaac aaccgcatcg atgcagatca gtccacattg
     9061 ggcgtgggga acatcgcggg acaggatccg ctttttgtgg ccgcgccaaa ccccggtccg
     9121 gatggagcgt gggaaacggt cgatgatgat ttcagcgggc tggtgctccg aaccggttca
     9181 ccagcaatcg acaaaggagt cacacaatat gtcgcaagta atggtgaacc aattccaccc
     9241 aatccgatta ccggctatac cggcgccgcg ccagatctag gatggagaga attcggggca
     9301 ccaatatttt tgacaccgac cgcgaccctg atctcttcgc tcacgccgct gcccacgttc
     9361 acccccatga ctgcgactcc cacatcgaca atgacagcca ccccgcccgc cacaggcact
     9421 ccaattacgc ctacggttac accgatcacg cccaccgttc aatccacgaa caccgtgacc
     9481 gcgccctcac cgacgaccat ccccgcgact gcaacggtgg ctgttacatc ttcaagcctg
     9541 gagattcaag gcgttacccc taactcagct caggcagata cggtggttgc acttaacatc
     9601 gttggcgccg gttttcaaaa tggatccgtg gttacattcg aaggtggtgc cggaggcaat
     9661 caagaaatca cgagtgtcca ggtcgtaagc ccgaatctca ttgtcgtaaa tatgacagcc
     9721 aggaatgatg gcaccgccgg cacacaggtc tgggatgttc gcgtcaccaa ccccgatgct
     9781 tcctctgcgg tactgacgga cgggtttact atttatccga ttccatagta ataaaccaaa
     9841 tcactataga tttgacttaa caaacaagga ccacctccag ttgcatgaaa ctcggaggtg
     9901 gtcctcacta ttattttctc acagcaagct tttagcaatc acttggtccc tgctgccaat
     9961 tggtagaggc ggcccattct tctgcgcctc ccatgatgat gtcgcagacc gggtctttga
    10021 tatcgtaata cgcttccata tcctgctccg ggtgatattt gacaatcgct tctttgacct
    10081 gaccataagc ctgagaaatc gtcgggaagg cgcgcagata atcgcgaaac aaaatagcat
    10141 agcgctggtt ggcgcggcca ggcaaccgta catgtgcatt tacttttcta ccgggtgagg
    10201 tgggttggaa aaaccacttt tcccactgat atgcttctga ttctttgccc ggcggaacat
    10261 gatcctgatt gatatgaact agtcgttgat acccggccag gttcatcgca cgttcaagtt
    10321 tttcagtgag tcgttcggct gtgatctgaa tatcgatcac gtcttttgcc gcgagcccag
    10381 gcacagcagt ggatccaata tgatcgatac gcagtgccag atcgtcgagg aattgtctca
    10441 gtagggttcc tttttcaaga aattcattcg accaggatgg tttgtattgg acgattgaaa
    10501 tcaagtgatt tctcctgtta ttgcaaaggt tggaatcaaa acaccttgtg acgttttatc
    10561 acaaggcgtt cgatatcaaa attatatctc agacctgttg aggttatcgg tctacaaggt
    10621 attctattct acggcagatg tgtctcaaaa aaatgtaaca cctgggcatc cgccgcgatc
    10681 tttgcatcca catccaactg ggtgaatcca tcttctgcct tagtggctcc atcaaagcgg
    10741 tgctgcgcat cctcaaaagt gttaatggtc acattcgtga cacccaattg ttgagcccca
    10801 gtgactcgtg ccgtgcaggc cgctggatca gtaattgcat ccactgagcc ttgcacgatc
    10861 accaatggcg cataaggttt ccaggtgctg tgcttcaaaa caccaagtgc gttgccgaaa
    10921 gtgcatccgg gatagaacgc aactgcggct ttgaactgag cctcatccac agattggctc
    10981 tcatccattg ctgccatcac ggagctgcca ccatgcgacc agcccattac accgacctta
    11041 tcagcagcca cataggattg tgtaacaaga tagttcagac cggcatacgc atcataaggg
    11101 cgaatattta tttcagatct tcctgtggta ctgttgccac attgatcctg agaatcgacc
    11161 aaaagggcaa catatccggc agtgactaac ctgtcacccc attcaaggta catcgagctg
    11221 atttctttag cgcccgtgcc ataagcatat gcacccatac agccgggcat catcaccaca
    11281 gcgggaaatg gaccgttgcc atctggtttg aatacccggc ctgtcagacg aatcccttga
    11341 gaattcacaa aagtaacctg ctgtggctta acattcaagg cgccggcaac aaaagcgggt
    11401 attgaaaagc tgatgagcag aatcatcccc acaaagaaag aggtccgttt gagtctcatg
    11461 gttcatactc cttgtgtgat ttgttatgcg agacagtcgg agtatagcga gcggcttgct
    11521 atctatgatg agatttaccg aaaagaatgc tgacaaattt gttatcgaaa cctctaacaa
    11581 agatgttgac agccagccgg cataaaaacc gggttaatta cttattttta gcaaacatga
    11641 actatctggg ataacaaaaa gagcttcacg ctgtattgcc caccatatcc catacaccta
    11701 tacatgttca ttccgtaggg agagatcgat caaatttcca aagatgccga ttaggaaaga
    11761 gacgctcagc gcagtgaaca ccacctggat caaaggccat gcaggatcat atcgaaaaaa
    11821 aggggtcagg tccacgggcg aagcggcaag atttaggtga atcagccacg tccccagaat
    11881 cgaaataaaa actgaaacgc tggaaagaag cattttgaca agcttcgggc gtatcaatcc
    11941 gccaatggcg aaggtgaatg caatcagcag agagccgagc gtaatcaaaa cgccctgagg
    12001 cggggtacca agaaaaagca tgtgaaaaat aaatatggca atgtttattc caagcccgcc
    12061 cagaatcgtc cctaacatga gacttagata cgtgttcgat gaatggaagc gctcaacaat
    12121 gactctcgtg agaaaaatgc ctaatcccag aatcgaacca atgattagtc cacgaaatag
    12181 agaggccaca atacgggcaa catccatgaa ggttggaaga ttataggtta agtaaacttg
    12241 caaactgact cccagggcag acccaaggaa tatcgtccca taggcaccaa tcaggttaac
    12301 cggtcgttga gtcattcgtt gcagatttcg ttcgagtgag agccgaaaac gaacgggacc
    12361 aggcaccaaa gaaggcaggc tgcccgctac cttctggatc agtaacaatg catcaatttt
    12421 tcttccttca ttggagtggg tcaatacgac cctgacggcg gaaggggaac gaatgtgacc
    12481 aatcagttcc gctgcggaat caccgaattc cgaatcttcg agcgccagca cgcccaaccg
    12541 cttgatatga ttcgagtcaa gctctggatc tttccataca ctccctggtc gcaagaggat
    12601 acgtagcccg gtaaaaattt tctgacgaag tatggcgtca ttcgtttttg ttccaagtgc
    12661 caacaatgag ttcgcaagat ttttgggtaa tccattggag gaagaaagga tatgccgatc
    12721 gcccgtcaaa cgatcaagaa ctcgcccggt gatcgcctcg ttttccttcc tgagtaaggc
    12781 ggatgaaacc atcaatcgtt cgcgagggtt tctaacaatc gaccaccatt catcatcgta
    12841 atgtccatag gtcaacgcct gagatagcat aaatcgatta aaaacatccg tgtcgctttt
    12901 tatcccctct gcattaatca gcgcaaactt cgtcaagccc aggttgtgct taccatccgt
    12961 ggattcccat tgagtcaggc cgctgttgag gatttcttct gcatctttgc tacgggaagt
    13021 ggacccatca tatggtctct ttgaactcat ggaatcagcc gggagattga agacgtaatg
    13081 catcatcctc atcacctcac tgccagaacg tggccgaaga tccggattcg cagaagtcac
    13141 gcgccgcagt acatctgtaa tcagaggtgg aagcccggta atatattcgc gcgggtcggg
    13201 tagctgctgg tcagaatgga gctgctccac tcccaattgt cgtttcccgt tccaggggag
    13261 ttgacctgtc aacatctcaa aaagcagtat gccaaaactg aaaatgtctg actttgcggt
    13321 gatttctttt gaacgaatct gctccggcgg agcataaggc ggagttccgt gcccggtgtg
    13381 gaacgccaac gtagagtcag aaatgatacg cgccaagcca aaatcagcca ggtatgtatg
    13441 atcgctgaga tcgagcaata tatttgatga ttttagatca cgatgaatga caccttgttg
    13501 gtgaagatag tccagggcag aagcaatttc cgtcccatat tttatgacct gctcgaatga
    13561 taagggcgac ttgatccgtg cggttaaggt gcctccggga atatacggac tgaccataaa
    13621 acgcacgttc ttttcagatc catattcata taacggaagg atatga
//
